LOCUS       DAELXX010000001        95614 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.001, whole genome shotgun sequence.
ACCESSION   DAELXX010000001 DAELXX010000000
VERSION     DAELXX010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 95614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95614
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.001"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(550..2226)
                     /locus_tag="KPH29_000001"
     CDS             complement(550..2226)
                     /locus_tag="KPH29_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG1465650.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIDGYSLEAQKSRMKAFALYNDYEIV
                     GEYEDAGKSGKSIEGRVQFTRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDFGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRVQTMEGRIQKAKEGKWNG
                     GFAPYGYQLIDGKLIINEEEAIAIRTIFDQYVNTSIGANGLSKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYRQVEQDDYLVAEGIHEAI
                     IPDELWQAAQVKLKAQAKKYEHVNKGKNMRTHLLSGIVKCPICGAGMFGNKSIKYKKD
                     GTKYKDFYYYGCKHRLMNRGHKCTYNKQIREELLDDAVAEVIIKLVSNPKFASMIQEK
                     INMKVDTSAIENEIDNYQNELRKSHSTKYKLIEEIDNLDVEDKHYKRRKNDLDDRLYR
                     MYDKIEELESQLIEAKAKKETIEVEKLTGDNIYKVLIYFDKLYKVMNDVERRQLIEAL
                     ISEIQIYEEKQPNGQWLKSITFKLPIIDEDLNISLENDEQVETVAKIIKQ"
     gene            complement(2327..2479)
                     /locus_tag="KPH29_000002"
     CDS             complement(2327..2479)
                     /locus_tag="KPH29_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1465651.1"
                     /translation="MDNDWNGLADLIANLITKYAGVLDLENLPNPTPVKNTEDKNKFD
                     IAKSTD"
     gene            complement(2968..3378)
                     /locus_tag="KPH29_000003"
     CDS             complement(2968..3378)
                     /locus_tag="KPH29_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG1465652.1"
                     /translation="MAKEYYLYIRGQKVKVSEDIYIVYWREKEHEKYLEQVDRKNHLL
                     FFSALDHDGHFTDNIVDESVDVEKIVETQMMIEAVRSAITRLNAEERDIIERLYFNDE
                     TVRSVAKLKSITHPALIKRRNKILEKLKKFIEEL"
     gene            complement(3902..5221)
                     /locus_tag="KPH29_000004"
     CDS             complement(3902..5221)
                     /locus_tag="KPH29_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1465653.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLVFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLELYSKSSEIKAFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKTRLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKALQDEEFFIFKIKIFS"
     gene            complement(5218..5871)
                     /locus_tag="KPH29_000005"
     CDS             complement(5218..5871)
                     /locus_tag="KPH29_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1465654.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYNDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            complement(5969..7345)
                     /locus_tag="KPH29_000006"
     CDS             complement(5969..7345)
                     /locus_tag="KPH29_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1465655.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKIFQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSSKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDVHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFVGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIARQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFIIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(7345..8001)
                     /locus_tag="KPH29_000007"
     CDS             complement(7345..8001)
                     /locus_tag="KPH29_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1465656.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQKKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(8011..9288)
                     /locus_tag="KPH29_000008"
     CDS             complement(8011..9288)
                     /locus_tag="KPH29_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1465657.1"
                     /translation="MIKNAIAYITRKRNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIGENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNKSENNKIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKHIIKIMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMLNITTLGQ
                     SYLILISIIVLSVVFASSLILIKKPKEILSKIS"
     gene            complement(9307..10197)
                     /locus_tag="KPH29_000009"
     CDS             complement(9307..10197)
                     /locus_tag="KPH29_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG1465658.1"
                     /translation="MDRIKNIINGSTKKDKYKIRDRFRHLFQSVWFLITNSYFEGFKT
                     GKIYGGNLKKICVPGMNCYSCPGAKGSCPIGSLQAVIGNSKYKISYYIVGILFFIGAL
                     VGRFVCGWLCPFGLIQDLLHKIPFFKKVETFKFDKHLRKLKYIILLVFVIILPLFLVD
                     ILGQGSPYFCKLICPIGMLEGGLPLVLLNKSMRNAIGFLYYWKGIILIVTLLLSIIIY
                     RPFCKYICPLGAIYSIFNPISIFRYRLDKDKCINCGKCKKVCQMNIDPVENCNHLECI
                     RCGRCKNACPVDAISYGVRK"
     gene            complement(10190..10285)
                     /locus_tag="KPH29_000010"
     CDS             complement(10190..10285)
                     /locus_tag="KPH29_000010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBG1465659.1"
                     /translation="MAMIYYGHSNGETKVVLDKAIRICMECIGIG"
     gene            complement(10364..11521)
                     /locus_tag="KPH29_000011"
     CDS             complement(10364..11521)
                     /locus_tag="KPH29_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBG1465660.1"
                     /translation="MKKIVSRGACLLLTLCLSLSTVGCKKSNESNKIKEGQSISSEEA
                     GMSTNEKDKNETFKPSDYTLKTKKEYVYEYLGLKFKLSDKFKKYMDDKKIAMLDDQSP
                     IDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELERIGTIGIFEKNTSEEKISK
                     ITKCDTHTKIGVSSDGKYDCYLSTNSGAESNLLDELKRTEIQIIDKKERPKNGFVLSE
                     KTDLENTEAFNKESVKDLRKLSTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEI
                     PDLVEVQNEMKSKGVNIVGVVTDTVDDNGENKEAIEKSKLIHEKTKASYPFLMPDKTN
                     FNGRLNGIQAMPETFFVDSNGNIVGDTYSGAKSSKEWKQVIEKELEKIKNK"
     gene            11855..12205
                     /gene="mobC"
                     /locus_tag="KPH29_000012"
     CDS             11855..12205
                     /gene="mobC"
                     /locus_tag="KPH29_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBG1465661.1"
                     /translation="MANRIRNERLEIKLTEEEKALFEEKRKLARCRNMSHFIRKCVLE
                     KEIYQVDLEPFRDLQGLLSNATSNINQIAKRVNSTGIIYKDDINDMKKQIEYFSKELW
                     QIHSLLLNRTSGGD"
     gene            12211..13542
                     /locus_tag="KPH29_000013"
     CDS             12211..13542
                     /locus_tag="KPH29_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBG1465662.1"
                     /translation="MAITKIHPIKSTLNLAISYIVNGKKTDEQILVSTHKCHQETAHT
                     QFLRTRNDVGTNGTVLARHLIQSFLPGEASPKMAHQIGLELCKKILKDEYEFVLSTHI
                     DKGHIHNHIIFNNVNMVTGKCYQSNKKSYHKIRYQSDKLCKENNLSVIDEFYESYKRK
                     YKTNGKSWYENEQSKRGTSWKSRLQFDIDRLIKQSKYWEEFLKKMAELGYEIKYGKHI
                     AFKPKDKTRFTRAKTIGEDYTEERLKERITENQSITTPPVKRRIGNIINMNANAKVKE
                     SKGYEYWATKHNLNTMAESVIFIREHGINSIKQLDKYIQKSAEERQDLQDKMKEIDKD
                     MQLLSDTMEQVHTVKKYRAYYKEYKANPSDKAFFEEHKAEITHYEIALSKLKKSYSKL
                     PNSKDILDKLNKLQEKKNTLIQEYSSTKSTMDELYQIRKNYGIYMGKEMER"
     gene            complement(13578..14384)
                     /locus_tag="KPH29_000014"
     CDS             complement(13578..14384)
                     /locus_tag="KPH29_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009354872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HBG1465663.1"
                     /translation="MEISKKELRKISRKFRILASNVMNSYYGEQNANLEEMINYIEQT
                     LLIKEYLHSLEYDVDGLDDLLNTISNSYGRKVLNLGNDSNKRTYLLYRAFSFITGNNL
                     PTYTFGWYYAGSNKYQDMAKAFGDRMVYPFVLEVENYMKDISTDMGYDENNALYNINV
                     NSSGVQVNIAEHGSTINADQENKFNTEEVIKAINNVEDLIKGMEDDNSKSILNQNLDN
                     IKNEIKQKHPKKEVLTTCLKSMHFITTSVALIPDLALGIKMLASLIGISI"
     gene            complement(14374..14835)
                     /locus_tag="KPH29_000015"
     CDS             complement(14374..14835)
                     /locus_tag="KPH29_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBG1465664.1"
                     /translation="MANDIKFEISVPADEEGYVQLKCPSCSEKFMLTVEDIEAESTID
                     IWCPNCGLTHENYLDDDIYELAERIVENQVAEMLNDFSKNLEKSFRNSKSIKFKRGKE
                     IKKEAELPIGRKTGDYEEKKYLCCGKTARISSIKKLEGSYCPFCGEMIDGD"
     gene            complement(14863..15930)
                     /locus_tag="KPH29_000016"
     CDS             complement(14863..15930)
                     /locus_tag="KPH29_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009354804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HBG1465665.1"
                     /translation="MSGKLYIAAAGSGKTRKIIEDSLNTHKKILILTYTITNEQQIIS
                     RIKNKVGVIPNNITVSTWFSFLLKDGIRPYQGSRFKERIEGIIFVNGQNNTYVKKTSN
                     KYFAKDNRVYTDKLSECVVELNDKCNGKVFERISGMYDCIYVDEVQDMVGYDLEILKC
                     LIASRIEVIMVGDPRQCTYSTHSSKKYKKYSCGKIDEFLKNECRKLDVRIDETTLNCT
                     YRNNDIICKFANSLYPERTSTNFVRRAEDESDGVMIIDKADVEKYLQKFNAMQLRENK
                     RTSVNNEYPYYNFGESKGLEFQRVLIYPTQPMWDWIINNNTSLKEQSKARLYVAVTRA
                     ENSVVIVRKDARPCSLPVVKL"
     gene            complement(15927..17549)
                     /locus_tag="KPH29_000017"
     CDS             complement(15927..17549)
                     /locus_tag="KPH29_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG1465666.1"
                     /translation="MSIRKLKIKNFKCFSDWFIVDFENGINILVGNNGTGKSTILEAI
                     NLVLTGTYHGKNIRNELTQYLFNKNIVENYIREVNEGVNVEPPKIIIEAYFDAEMPSF
                     LGDGNSEKTKDTPGICLSISFDEKYSNEYKMLCKNKITSLPIEYYEVSWMSFARENIT
                     ARSIPIKSAMIDTTSYTPTSGSDIYISRIVKNLLDDNEIINISQAHRLMKEQFSRDEN
                     IANINRKLTETSNNELTISVDFGTKTSWESSLVTQLNEIPYNNIGKGTQCIVKTQLAL
                     SDHKIENKQIVLLEEPECHLSYSNLNILLDKVNESVKEKQIIVTTHSSFVSNKLGLEN
                     LILLNRGKTLRLGDLSNETNLFFKRIAGYDTLRLLLSNKAILVEGDSDELIVQRAYMD
                     SHNGKLPINDGIDIISVGISFLRFLEIAKLLELRVSVITDNDGNIDALNRKYSDYLGD
                     NCCENINIYFDDSTHEYNGSLENYNYNTLEPCIYRSNSMSILNEILNKSYEDPDDLLK
                     YMKGHKTEVALKIFESDKAIEFPQYIKEGILI"
     gene            complement(17752..17982)
                     /locus_tag="KPH29_000018"
     CDS             complement(17752..17982)
                     /locus_tag="KPH29_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1465667.1"
                     /translation="MRVFSYKRLFKKLIDLDMTNNELMEKAKVSKSTFYKMKNGQNVT
                     TDVLLRICNALDCDIEDIMECVIIEDVRRMGV"
     gene            complement(18122..18781)
                     /locus_tag="KPH29_000019"
     CDS             complement(18122..18781)
                     /locus_tag="KPH29_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006627214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG1465668.1"
                     /translation="MDYKTMRDRVEDMLSDNHKDFVKAIISMEKGINDESALDKLYDA
                     YMDNDSLNLLHEEFDYMIEDLRGQGQIKDLLYVREEKDNLINIVGNIVGEVDVIEREN
                     KNGETFKVANFSVVSKDDEGNKLYHNCSVYGEKSDIPKDFKQGDFVKLFGQIRTSIDD
                     NGKEHSNIRILSSKLLKAKEQMKGQEEKKESILGAIKKYQAEDKEKPKEKKEASKEAE
                     R"
     gene            complement(18824..27553)
                     /locus_tag="KPH29_000020"
     CDS             complement(18824..27553)
                     /locus_tag="KPH29_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBG1465669.1"
                     /translation="MRINDFHNILELIKQDVLYNEAEYLKLLKVVGNNQRYDFRSQLS
                     IYDKNPEATACAKFDYWRERFNRTVMRGQKGIPILEDYGTYKKVDYIFDISQTVSKNR
                     DVNEVNLWRFDKEAHQDVLKEMIKSEGYEESESTLENIFSLSRLYGDEKIDTLMNELR
                     VADEDRISFTKFVRDSVSYAVASRFKLDYPMDYELLRENFQRFDSISLMSLGESVSDI
                     SGKIIDETIQKSKELELQKEVLIGKEAGYNKIKEELEEVEENVFRRDDQERNENERVL
                     RNGEYGRDNRKNQGEYAKQLGGTDGLYKEVSESDLRSDEAELPFTERGAEPLRDAFGS
                     IQGEEADRTPDGYSETGSRVYESREAKVDSSLEDRGREQSAVWGDDFSSQRNDHQGSS
                     GDLKDNTDKGIIEAEKASFSLPENSYGQISLTIPLTEKDIDTVLINGGNHDGGRLPVI
                     AEFSKGKSIEELGEYLKDTFKGGNGFYIDEREVSSWYSDKGIHLAYGTSAREDNTQIL
                     SWSDAASRINELLDRGEFATNVELLEAQDYERDRISESLWYLTHDLSEEGIEQGYFNS
                     FERGGGFPQETKRLSEALKNPEYLKETIKEYSRFLEGYKESRNVLRYHYHKVDSLYQK
                     LQELELQRKEYSTNLTELPKVKLFITEDEVLESLSRGSGVDRGKERITKFFKENHTLQ
                     EKANFLKDEYGIGGHSHVVSGAMGSDEWHDAKGFKLQKNDCNDVFLTWTSVAKHIDEL
                     LSKKLYFEEKETESTAEIEEVKEPQYYSKNDPGNLMPDEILERVPEIYAQEDVDLADK
                     QVHAAYIIPFRSNWTWYMTEYDRESGDAFGLVLGIEPEWGYFNLEELKELNAQRLILE
                     DFPKTFRELKDIELIKQMDEQELQFVFNGELSFEDKVELEAPEETEEATKRGTVQATL
                     FDYLKEREEVELNEKEDSLLEEFAVKAGDTVYFNHEEYKVREISKNEITGRYDLWLDP
                     VRSGNHQIPIVDFTDNEDLLNKISLERPNFIVGDEVKYKDKNHTITRFDDMRKNLNTV
                     TVKDNTEYFGGMITSFDVIPYRLESDLERVFENLTYKQPEQTTEETQIRKAEAHNFKI
                     TEETLPEKLSPSERLNNNLEAISMLNRVESGQRELDSTAQEVLARYVGWGGLSEVFDE
                     SKEGQWKEARAFLNENLSHAEYEAARESTLTSFYTPKTVIDGIYKTLSDMGFKQGNIL
                     EPSMGIGNFIGNIPDEMNKSKFYGIELDSVSGRIGKLLYPESEVQIKGLEETSFSNNF
                     FDAVIGNIPFGEYKVNDREYNKNNFLIHDYFFAKSIDKVRNGGIIAFITSSGTMDKKD
                     ESVRRYLAARAEFLGAIRLPNDTFKGIAGTEVTSDIIFLKKRDSIRERDEDWIHLAED
                     ENGLTYNKYFVDHPEQVLGTMREVSGRFGNTLACLPKENADLKELLTKASEEISKGST
                     YEEIELLDDEITSIPATDDVKNFSYTVIDDEVYYRENSLFIKKEITDKNKEKIKDYLE
                     LNTALKDVIYKQKEDYSDDEVKKAQEKLNEVYDNFSKKHGFINNLSNTRALKEDSNFP
                     LVSSIEILDEEENVKAKGDIFSKRTITKAKVIDHVDTSLEALVLSVSEKGYVDFEYME
                     SLTGKDRPTLIEELRGEIYLNIREEQNFYRPLSFNLEDGDLPFACANGSNSYKYGYVT
                     KDEYLSGNIRDKIAIVDSYLAKLRQTERELPHLGFVENGKEKELISYEMNRLEYQKAE
                     LTKVLPKELEASEINVRLGATWIPIKDIEKFIFETLKTPGYARWDIKVKFSNITSEWN
                     VEGKSRDKGNDLAEMTFGTSRVNAYKLIEDALNLKETKVFDQIINPDGSKTSVLNKKE
                     TMLAGQKQELLKEEFKNWIFNDQERRNRLVKLYNERFNSIRNREYDGSNLSFEGMNTE
                     IDLRPHQRNAIARSLYGGNTLLAHVVGSGKTFEMVASAMESKRLGMCSKSLFVVPNHL
                     TGQIGREFMQLYPSANIMVADKKDFEPKNRKRFIGKIATGEYDAVVIGHTQFEKIPMS
                     KEYQEKHIQDQINEIVNYVEEYKHDRNQNFTVKQLEKTKKKLETRLEKLNDDFKKDDV
                     ITFEELGVDMLFVDEAHNYKNLYLYTKMRNVAGIGQSEAFKSSDMFMKCRYMDEMTGG
                     KGIVFATGTPVSNSMTELYTMQRYLQYESLKKNHLEHFDSWASTFGETQSSFELSPEG
                     TGYRVKTRFSKFYNLPELMSMFKEVADIQTADMLNLPTPEANYEVIKTEPSEEQKEIL
                     KSLSERADDVRNRVVEPDVDNMLKITNDGKKLALDQRLINPLLPDNPDSKVNVCVKNV
                     FAIWDKTKENRSTQLLFSDMSTPKGDGEFNIYDDIREKLVAMGIPKEEIAFIHEANSD
                     KQKDELFAKVRKGEVRILMGSTQKMGAGTNVQNKLIALHDLDVPWRPADLEQRAGRIV
                     RQGNENEKVNIYRYVTENTFDAYLWQTIENKQKFISQIMTSKTPVRVAEDVDESSLNY
                     AEIKALATGDPKIKEKMDLDNEVTKLKMLEANYKSNRYRLEDKVAKNYPEEIARTEKL
                     IEAVKKDISNVEPKAEGEEKFTSITIAGEKITDKKLAGERLLEAISKVKINESKVIGK
                     YRNMDLEVSYNFFTNSHNFSLNGVAKHLGELGTSADGNITRLDNALEKMPEKLNMLEE
                     KLISTKEQLENAKEELKKPFEKADELKTKVLRLAELNKLLDMGEVEEKKNDNPLVEDV
                     KRAIIDFCNREYEENHSYDEFHTLYPDLKHIGIAYTNTPDERHGIQYELNLEEKTWTQ
                     YIDDTPIKTESFDYENKGENEALRNMKNEIELSTFSDLVYVDSEDLRVATGLDIDDEG
                     NFYDPFAKDLDNDGIPDRYDSDFKDSDYFESTYDVEDNLHSKEETTQKSDDKPSILGQ
                     IRAYQEESKTEEKQKIKEQEFLR"
     gene            complement(27636..28439)
                     /locus_tag="KPH29_000021"
     CDS             complement(27636..28439)
                     /locus_tag="KPH29_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004804477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HBG1465670.1"
                     /translation="MSKINVEGSEISIIAIDNRDYISLTDMVRNIENGSALIEKWLRN
                     KNTIEFLGIWEEMYNTNFNSPEFEGIKNEAGFNRFILSVKQWVEKTNSIGIVARAGRY
                     GGTYAHKDIAFEFASWVSPYFKLYLIKEFERLKEEEQKKLGWDIKRNLAKINYRIHTD
                     AIKNKLVPDKLSKEKINFIYASEADILNVSLFGITAKEWRDENPDLKGNIRDYADISQ
                     LVCLSNLENLNAVFINEGMSQSERLEKLNAIAIEQMKILSESESIKKLK"
     gene            complement(28524..30230)
                     /locus_tag="KPH29_000022"
     CDS             complement(28524..30230)
                     /locus_tag="KPH29_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HBG1465671.1"
                     /translation="MKLVIAEKPSVAISIAKVIGANKKKDGYYEGNGYRVSWCVGHLI
                     QMANPDAYDEKYAKWNMADLPIIPSDYKYEVAKATKKQFNILKKLMNDKEVDTVINAC
                     DAGREGESIFRLVYNQVNCKKKMKRLWISSMEDSAIKEGFDNLTDGKDYDNLFESAQA
                     RAIADWLVGMNISRLYSCLYQQNYSVGRVQTPTLAMIVKRDDEIANYQKEKYYTVELS
                     MNGFTLSTDRIDDEVAAEQLISLVGDKIEITDVIQKEKITKPDLPFDLTTLQRECNKY
                     FGYSAKQTLDYTQSLYEKKLITYPRTDSRCLTEDMIVSTVNNILGKNDFDTERIKVVF
                     NSKKVTDHHAIIPTISSVKEDVSELPLSEAKVYFLISDKFHASVGYPLIENTTKIVAS
                     FDGFEFTSSGKVIKDEGFSKYLKEYKSKKSEDAVLPDVSVGDVLSVENKEVKEKFTQP
                     PKHFTEDTLLKSMEIAGNDALEKGVEVERKGLGTPATRAGIIENLIFKRFVERDKKNL
                     IATHKGISLVTIVADTFKSAETTAKWEMELSDIAQGKSSKKEFLDAIENEIKEAVLTY
                     RK"
     gene            complement(30325..31866)
                     /locus_tag="KPH29_000023"
     CDS             complement(30325..31866)
                     /locus_tag="KPH29_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HBG1465672.1"
                     /translation="MKTSLKKNNKFWTVALAVIVSISCILGLWTVVYAQEQKSEAPTK
                     NEAVQTEVEVNVRYIFEDEKVFKEEKIKAEKGQLIDSGDLPMLPDDMKFIDEFLFYEV
                     KGDGKDEIIRKVAKMEVKDKETQTEEEKPKQDECTQTEDKGTQTELSKDDISKIEKEA
                     KELQDKLDKLNCELKDKDKLSDKQKNKIKDLEDEIDSLKEKMKKDKGNKDLSEDMKKE
                     IDKLTEKVKELEKKATETNKAPVTSQSVTPISPISGIKTSSGISPQTPQTSVSSTGKG
                     SSDSVSSGNTTKDTGKGKTEVKEKEKEVRYPNKLTAKAPANNSQDSSMGSASKSVNTN
                     KGVASSPSKARASVTENKDNANKEYPIHHGDSSDNKETDQYSADARQFITFQTKNGKT
                     FHLIINHDEDSENVMLLTEVSEDDLLNMVEKKEAPKQEVVKEEPVKEEVKPEKKDEKS
                     NLGTYIILLLVVGGALGAGYYFKVVKKKEDKELEALEEEDDDFFSESESEEEINDADE
                     VEDEE"
     gene            complement(31841..32080)
                     /locus_tag="KPH29_000024"
     CDS             complement(31841..32080)
                     /locus_tag="KPH29_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG1465673.1"
                     /translation="MKKELTTVKNRIKKLKDKKALIDEELEPLFIREEELENEEIIAI
                     CRKNNITISDLIAKVNRQKAEMKKEKTNENQFEKE"
     gene            complement(32092..34224)
                     /locus_tag="KPH29_000025"
     CDS             complement(32092..34224)
                     /locus_tag="KPH29_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HBG1465674.1"
                     /translation="MGKKLKKDFKERHKASLEREMFHSKTDTIPEESKLKHNDDYRGK
                     IVHEKERFQDKVHEKTSKVSADNEKSYGTSKRNEKYRTSDKGDVNSAIETGRSDYITE
                     VKDGKIYDPLGKDLDNDGIIDRYDNDFRDSNYFESTYDVEDNLSHKEKSLENSFKNHK
                     TQKSRYKRKNYSESLYTRKKDDAPKGNKADDKKAGKDAVSGKVYSSLSKDQKKKLKKD
                     MVKVSALSGLAKGSETVRDYLSHGSDENQGVEAGEKTADTSSKLIHGIKKYSDKKRAK
                     RGYDLTNKDYKIRKRKSKLEFRDAKKELKKTDEYKRASAYKRFQKKNQVKAAISRENK
                     SRLRDRIKEGLIGTLKSSKDMIIRKAKGLMLIFIGIIILGTFVINFAGTGMTGFMNST
                     SSVLTTSYLSKPNVLSEINQKFSDMEGELQSEVDYVKENYPGYDEYILNNTEYIGHNV
                     HELLSYITSRCGEVKSVSEVESILKELFESMYDLEYREEIEIRYRTVTETYTDEDGNE
                     YTESHEEPYEYKKLIVTLHKKEMDSIIRKVFANYPDNLKHYEALFLAQGNMREAFGNS
                     DLISANGGIGGGKEYEASTEVQKKIVNAAYITPSPGAGWCAMWVSQVYQNAGLGYIGG
                     NACDMYRNYTFTSDRSKLKVGMLVAVESSSSGSSAGLTYGHVGIYIGDGKVIDNIGRI
                     RVTTLDDWIATFCKHHPVGFGFPPNVKK"
     gene            complement(34228..36657)
                     /locus_tag="KPH29_000026"
     CDS             complement(34228..36657)
                     /locus_tag="KPH29_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG1465675.1"
                     /translation="MNKKRKIQQIKLMQNEKALRSQKRDLKKSNCKSKKDKGGILDLI
                     FKKEPKRYTVEDTIPYLRLLKSGICQLDEKHFSKSIAFQDINYQLALDEDRDLIFNQF
                     ANFLNSFDPSISIEFSYINQLGRNEEMKSAIQIPDKKDGFDDIRFEFREMLKSQIVKG
                     NNGLKKSKYVTFTVEADNLEQATSKLERLEIDILSSLKSMGVRAESLNGEERLKILHD
                     VLNPNKTFKFSYKDLKKRESTKTYIVPDEFNFTPSRYFKFGKFIGATSHFQILASELS
                     DRMLSEFLDIDDNINISFHIRAIDQSEAIKMVKRKNTDIDKMKIEENKKAVRSGYDMD
                     ILPSDLITYGEDVKSLLKDLQTRDERMFVVSIVFMNFARTVQKLDNTIAQISSIANKH
                     NCKLKRLDHSQEQGLVSVLPLGVNKIEIDRGLTSSSTAVFMPFTTEELFINSSNSLYY
                     GLNALSHNLIMADRKKLKNPNGLILGTPGSGKSFSAKREMANAILVTDDDVIICDPEG
                     EYGNLVRQFKGEVIKVSSKSKDYLNPLDINMNYGDGDAPLKDKANFIMSMLELVVGGS
                     GLTAEEKSVIDRCLPKIYEKYFEHPTPDNMPILQDLYDMLKGQEEKVGKKLATEMEIY
                     VSGSLNVFNHRSNVDLNKKLLCFDIKELGSQLKKIGMLVIQDQVWNKVSQNRGNKATR
                     YYIDEFHLLLKDEQTASYSVEIWKRFRKWGGIPTGITQNVKDLLMSKEIENIFDNTDF
                     VLMLNQASGDREILARKLKISLPQLRYVTNSNEGEGLLFFGNTIVPFIDKFPKDTILY
                     QKMTTKPEEVR"
     gene            complement(36569..36958)
                     /locus_tag="KPH29_000027"
     CDS             complement(36569..36958)
                     /locus_tag="KPH29_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HBG1465676.1"
                     /translation="MAYVPIPKDLNRVKTKVAFNLTKRQLIGFTLAGLVGIPVYLFMR
                     KFVPNDIAVIFLIVSTLPIFFITLFEKDGLTFEKYFKYIYLHKFYQPQKRVRKEVYLE
                     QEKKNSANKTHAKRKGIEKSKAGLKEK"
     gene            complement(36962..37222)
                     /locus_tag="KPH29_000028"
     CDS             complement(36962..37222)
                     /locus_tag="KPH29_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG1465677.1"
                     /translation="MNKRKTVFIVVIGAGIMAIIDRIRLHNKVEELEEKTEDIGRCHN
                     DFYLIQQRYNKNTDEQLATIQEEIGSVYEHFEELSKGKEDGR"
     gene            complement(37240..38103)
                     /locus_tag="KPH29_000029"
     CDS             complement(37240..38103)
                     /locus_tag="KPH29_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1465678.1"
                     /translation="MFGIFDKIEEFFKELLLGGIQANLESMFLDINDKVGAVATDVGK
                     TPMGWNGDVFAFIKSINDSVIIPIAGLIITAVLCIELINMVMQKNNMHDTDTFEFFKY
                     IIKMWIAVWLVSHAFEFSMAVFDVAQHMVNKAAGVINTSAVISGDQIVKMVDGLKDKG
                     LGELVMILFETSLIKVAIQVISIVIMLVVYGRMFEIYVYSSISAIPFATMGNKEWGQI
                     GTNYIKGLFAIGLQGLFLMVCLGIYAVLVKTIQITDIHTSTFTILGYAVLLGLMMLKS
                     GTLAKSVLNAH"
     gene            complement(38123..38338)
                     /locus_tag="KPH29_000030"
     CDS             complement(38123..38338)
                     /locus_tag="KPH29_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HBG1465679.1"
                     /translation="MEFFAQAVNVLKILVMAVGAGLGAWGVINLMEGYGNDNPGAKSQ
                     GIKQLMAGGGIVLIGLKLIPLLANVLK"
     gene            complement(38342..38653)
                     /locus_tag="KPH29_000031"
     CDS             complement(38342..38653)
                     /locus_tag="KPH29_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG1465680.1"
                     /translation="MKQEMININANLVAEPTFSSFEKEGEEVEVVNFTLVKKYGKGKE
                     YINCAAYGEKAEKAEDFEKGDLIHVFGYFKKREKEGKTYKNFVVKSYNKIEKKEENEE
                     E"
     gene            complement(38817..39050)
                     /locus_tag="KPH29_000032"
                     /pseudo
     CDS             complement(38817..39050)
                     /locus_tag="KPH29_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
     gene            39192..39365
                     /locus_tag="KPH29_000033"
     CDS             39192..39365
                     /locus_tag="KPH29_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1465681.1"
                     /translation="MDYMTLKEASEKWGVSSRQINYYCTDGRIPGAVKMAGVWLIPKE
                     AEKPKDRRYNESR"
     gene            39370..40080
                     /locus_tag="KPH29_000034"
     CDS             39370..40080
                     /locus_tag="KPH29_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1465682.1"
                     /translation="MLHIAICDDEKDFVQYLTDLLNQYSKETGRDIKITPYYDGMELI
                     EKYDPTIDLIFLDIQMKVVDGLHAAERIRQMDETVGIIFLTTLTQYGLEGYKYHATNY
                     IIKPMKYARLKSEMDKFLERSQKEDTPSLVITNDTGKYKVPLKSIRYVETYNRNLMFH
                     TEQENIICYKSMKEVERELCDKDFVRCHTSYIVNLFYVKGIKKLDIELITGEIIPISQ
                     PKRKEFMERLTDYWGDML"
     gene            complement(40351..40512)
                     /locus_tag="KPH29_000035"
                     /pseudo
     CDS             complement(40351..40512)
                     /locus_tag="KPH29_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569186.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            41123..41308
                     /locus_tag="KPH29_000036"
     CDS             41123..41308
                     /locus_tag="KPH29_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG1465683.1"
                     /translation="MLKKYWIKCPICNGKTRVQVFHNTVLKDFPLFCPKCKLTHIIDV
                     EKLEIVIKNTEKQTFII"
     gene            41325..42308
                     /locus_tag="KPH29_000037"
     CDS             41325..42308
                     /locus_tag="KPH29_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBG1465684.1"
                     /translation="MKRLPKYTPAEVRNDPYGFTYKEMSEVIGENEAKALYEELYKQL
                     PRKKNLSMLVKNICKSSDTEKYVYELKDNKYIETVFIKRRDGGTVCVSTQVGCPVGCI
                     FCESGRNGFVRNLTSSEIVQQIILLRRKVNRIVFMGMGEPLFNYDNLIKAIHILRDRY
                     GLNFPTDGITISTVGPVDQLKKLREEHLKIQLTISLHAATQSARNRIIPHMRIYAIED
                     VVKQALSYSERHNRKIVFAYLLLPGINDRPSDVRQLAKWFRGKKVMINVLQYNPTSNS
                     RIKAPQKREIVAFKHQLEQAGLEVTMRVSHGREINAACGQLANTYNKFKKK"
     gene            42782..43219
                     /locus_tag="KPH29_000038"
     CDS             42782..43219
                     /locus_tag="KPH29_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003508282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG1465685.1"
                     /translation="MELSSSDKERIQHQYDALAKKTLVGEAKSHRRTLAKRAAREVTF
                     SDLSESELAQLFTTDEYESDYFRFQVSGFDVLVKNELLAEALNALPERKRDIILLSYF
                     LDMSDAEIGELLNVVRTTVFRHRKSALAKIKQYLEGKADDEYR"
     gene            43203..43445
                     /locus_tag="KPH29_000039"
     CDS             43203..43445
                     /locus_tag="KPH29_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1465686.1"
                     /translation="MMNTVRKSENLLPFPVISAAANGDTTAMCAILKHYEGYIAKLCT
                     RTLKDDAGNTYSYVDEEMRNRLQVRLITRTLAFHVG"
     gene            43904..44077
                     /locus_tag="KPH29_000040"
     CDS             43904..44077
                     /locus_tag="KPH29_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-encoded TnpW family protein"
                     /protein_id="HBG1465687.1"
                     /translation="MDKKQTITTERKIGKITYLVQALPSEKATDTIHKKIEKLIVKDL
                     QKKPGNPGFLASE"
     gene            44155..45828
                     /locus_tag="KPH29_000041"
     CDS             44155..45828
                     /locus_tag="KPH29_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG1465688.1"
                     /translation="MLQSNKITALYCRLSQEDMQAGESGSIQHQKMILQRYADEHHFL
                     NTKFFVDDGFSGVSFEREGLQAMLQEVEAGRVATVITKDLSRLGRNYLKTGELIEIVF
                     PENGVRYIAINDGVDTAREDNEFTPLRNWFNEFYARDTSKKIRAVKQAQAQKGERVNG
                     EYPYGYIPDPNNRHHLIPDPETAPIVKQVFAMFVSGVRMCEIQKWLAENKVLTIGALR
                     YQRTGQARYQRAMIAPYTWPDKTLYDILARQEYLGHTITAKTHKVSYKSKKTRKNEEE
                     QRYFFPNTHEPLVDEETFELAQKRIATRHRPTKAAEIDIFSGLLFCAGCRHKMYYQQG
                     VNIEPRKFSYSCGAWRNRARTGSECTSHYIRKNVLLDLVLEDMRRVLRYVKEHEQDFI
                     CKATEYGDMEARKALAQQQKELFKAQARMTELDTLFRKLYEDNALGRLTDERFVFLTS
                     GYVDEKKSLAARIDELQQQIATVTERKRDISRFIQIVGKYSDIQELTYENVHEFIDRI
                     LIHELDRETNTRKIEIHYSFVGQVDTEQEPTQVVNHDRRNMVDVKSIAI"
     gene            45852..46307
                     /locus_tag="KPH29_000042"
     CDS             45852..46307
                     /locus_tag="KPH29_000042"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1465689.1"
                     /translation="MKEYTLIIKGEMDFIILSPQVLSSLITQIHNSPERKVVVSIESI
                     MPPKFTDYLLRVINSNRFSNERFRYRYILENPVTKKGMYEILRQQLSRTNTERFPCFQ
                     TIQLTDTFQGNVELDMECNDLFFWACKDTAAKFVYTFPDGREETLVIEY"
     gene            complement(46355..48007)
                     /locus_tag="KPH29_000043"
     CDS             complement(46355..48007)
                     /locus_tag="KPH29_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008978103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG1465690.1"
                     /translation="MSRKKQVYEKITALYCRISLDDGGDNESMSISNQKLMLRDFAEK
                     HGMFQYEYYVDDGYTGRNFNRPSFQRMIADIEAGKIGCVITKDLSRLGRNYIEAGSYI
                     EIFFPKHNVRYIAVTDGVDSLTRQEMDITPFKNILNDMYSRDISKKVLAGRMTRSRQG
                     KFCGGQPPLGLMRDPEDKGHLILDPETAPVIRKIYDMALDGWGCMRIAKQLMDDKIPI
                     TRVKSNTECDVNYYSWGSARISHILRNPFYKGAHLVCRTHQKGIRSNTYDIIPREDWE
                     VIEGCHEAIVTPEEWEQVQDIIDRRPTIMKGNACPFYNLFHGLVYCATCGKSMQVRYE
                     KVGRTGKNRFTGEMREPIDRAYYICQTYNRLGKNACTSHKIEARDLYNLVLKDIQELA
                     AQAMKDADAFYQRLSSRMERRYLVDASQTEKERKRLEDRNQEIDGMFLSLYTDKAKGV
                     LTEQRFMKLTAALEQEQEANQKRLHDLAVMQSRADAQESEVRTFIKEIRRYAAIAELD
                     ESVLNRLISKILIGEVKKVDGQKVQEVRIVYNFVGEIPEIAA"
     gene            complement(48080..48247)
                     /locus_tag="KPH29_000044"
     CDS             complement(48080..48247)
                     /locus_tag="KPH29_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008978104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1465691.1"
                     /translation="MEKSKNQKNKVRFVVVREFSGEKSMREAFEQLIERQTCEHFEEW
                     RHQREMAEKAA"
     gene            complement(48515..49873)
                     /locus_tag="KPH29_000045"
     CDS             complement(48515..49873)
                     /locus_tag="KPH29_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated protein E"
                     /protein_id="HBG1465692.1"
                     /translation="MSAAEALAPEQSVDEVREGLSVTEKGQPANTIGNCRTVFSHDPL
                     LRDAIRLNLLTDRVDIVRDLGWRRNTSALTDTDVKYLLLYFEQNYGLTSEKKMTAALS
                     IVANENCYHPIQDVLNGLVWDGTPRIRSCLHHFLGAEVSDYVEEMLKHFLLGAIRRVF
                     SPGSKYEEMLCLVGGQGAGKSSFFRLLAIRDEWFSDDLKKLDDDRVFLKLQGHWIIEM
                     SEMLATSSAKSIEEIRSFISRQKETYRTPYEAQPKDRLRQCVFGGSSNTLDFLPLDRA
                     GNRRFLPIMIYPEKAEVHILEDEDASRAYLLQVWAEAMTIYRSGKYSMKFSKSIQRQL
                     VEVQKDFMPEDTEAGQIQGFLEHYTGSMVCSKQLFKEALGHTYDEPKRWQLHNINEIM
                     NTVVTGWKPFSNPRMFAGYGRQKGWERDVSGNELPGNEDGFVELSEEECRQLELPKEW
                     IA"
     gene            complement(49833..50519)
                     /locus_tag="KPH29_000046"
     CDS             complement(49833..50519)
                     /locus_tag="KPH29_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBG1465693.1"
                     /translation="MKTGCLFLCLEVRKSNVFEAVKQAVPTRQAAESYGIQLGRNGMT
                     CCPFHDDKHPSMKVDRRFHCFGCQADGDVIDFTARLFGLNKKDAALKLAEDFSVGFDA
                     KGHDPPRRKPGKRKISEELRYRQAEQKCFRVLCDYLHLLERWEKEYAPQTPEEAWNPL
                     FVEALQKKPYTEYLLDILLSGSMEERACVVAEYGKEVRKIEQRISEFTASHPAGRHER
                     SRSFSAGAER"
     gene            complement(50535..51779)
                     /locus_tag="KPH29_000047"
     CDS             complement(50535..51779)
                     /locus_tag="KPH29_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/arginine repetitive matrix protein 2"
                     /protein_id="HBG1465694.1"
                     /translation="MKLTRHNGRSGKHGTYNPRHNDRRFDVENSEHIDAERARQNVYW
                     DCYRGFTTHEFRENPEQPDFSFEEIERMYYNEHYGGHVEAQNARNEKTRHTERNRTVE
                     DLLKNNKTCPEESIYQIGTMGESVPPDTLFSIVNEFYEEFERRFGSHIHILDWALHLD
                     EGTPHIHERHVFDCENRYGELCPQQEKALEELGIPLPNPEKPKGRNNNRKQTFDAVCR
                     TILFDIARRHGLHLDQEPSYGGRDYLEKQDYILMKQKEQLAAQEQKLEELTLKIEDVE
                     TLLDDVSDAAYDKAVEVVTDTVRQETHKEDIRLVEESKKWVLSPERKAPKKEREYAAE
                     RLDGVITKIKNAMQHALAKIQRTLMQPEVKQAGKEQVKKKAKESIMDILAKAKINADR
                     DNRERWEREGRIAPTKKNDIEL"
     gene            complement(52207..52512)
                     /locus_tag="KPH29_000048"
     CDS             complement(52207..52512)
                     /locus_tag="KPH29_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBG1465695.1"
                     /translation="MRKLSKYEKETIINWNEAENLASVYTFNASLKRRLADFSRKYPL
                     LCRLERSTPEGSVTYVLDKSRLSIRLVPPYSEERKAAASAYAKEHGFQVVGAEEKIA"
     gene            complement(52496..52789)
                     /locus_tag="KPH29_000049"
     CDS             complement(52496..52789)
                     /locus_tag="KPH29_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1465696.1"
                     /translation="MTLSMKEKKILYAYGCPSHHNTVTRLKWLTALTVDPEAKRRMLG
                     LARKVETEVGENWYEDFYHHLRMEMDEYRRLKRSLRVLKSYTDYEEDLYEEAV"
     gene            53155..54003
                     /locus_tag="KPH29_000050"
     CDS             53155..54003
                     /locus_tag="KPH29_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1465697.1"
                     /translation="MYAKLTIPERLKDLRVVDKHLTLEQLAEQTGLSKSALGKYETDD
                     YKDISPFAIATLADFYGVSTDYLMGLTENKNHPNIELQSLHLSDDMVELLSSGKINNR
                     LLCELATHPNFRRLMIDMEICIDQIANMRVEQMNLVLEATRQTVMNKYAPGEDDLYMR
                     TLELGQVQESDFYSHVMHDDLDSIVRDIREAHRKDKTTADPQPTLDDVKEKFEQAIGQ
                     GSDTEMLIHEFCDKLQIPFEKISSEDFSAFLRILSLSKQLKSSNNMRGKASPIPPNRK
                     ARRKRK"
     gene            complement(54125..54406)
                     /locus_tag="KPH29_000051"
     CDS             complement(54125..54406)
                     /locus_tag="KPH29_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015574404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1465698.1"
                     /translation="MILEEMYNGRFYPCETVVADSPRFKQAVKASAALMDTLSERLSK
                     EDYALVEELREQVAIAQCEENESHFKYGFSAGLLVQQEAHAQVSQKDKE"
     gene            complement(54420..54797)
                     /locus_tag="KPH29_000052"
     CDS             complement(54420..54797)
                     /locus_tag="KPH29_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HBG1465699.1"
                     /translation="MEHLPKKIHHNGISYTLVGDYYIPDLKLSEEIRPIGRWGRIHKA
                     FLQEHRPGQYNELLLSGKLWTYLADLNEQADDRLACIISQMQAAEGVTEELKARDQLA
                     WVGGMNSIRSRVEEIILSEMIFV"
     gene            complement(54895..56457)
                     /locus_tag="KPH29_000053"
                     /pseudo
     CDS             complement(54895..56457)
                     /locus_tag="KPH29_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
     gene            complement(56454..56939)
                     /locus_tag="KPH29_000054"
     CDS             complement(56454..56939)
                     /locus_tag="KPH29_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HBG1465700.1"
                     /translation="MINEEIARKTLNMEINAGKVTGKVLLNLLKKLMKEAEKFGGLEK
                     LVNANGNEVKLKDMVKKGQLEEIPVEEAELKELKKELNRYGVKFSVMKNKETGKNSVF
                     FQAKDMKVMDKAFKHALSKSEKKAERKESIHKNIEKFKEMAKNSISKEKIKNKQKEQS
                     L"
     gene            complement(56941..57963)
                     /locus_tag="KPH29_000055"
     CDS             complement(56941..57963)
                     /locus_tag="KPH29_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG1465701.1"
                     /translation="MYLFDEQPIVANKTLAREIGLNEALILQQINYWIEINKKSGKNY
                     HDGKYWTYNSIRAWQENDFDYMSFDTVKRTFSKLEKAGYLLVGNYNKDPRDKTKWYTI
                     NNDKLEELYMDMDLKKQKIQTEALKNSMPNALGQNAPMDKGKISQCNNADCTNAQVQN
                     ALMQKDNLHQPLPENTTNNTTNNTTNNSAYISIHPSIKEKNQLFSENMKGGRIDISYK
                     NIYQNCLEELRHQTGYYEHMSIGNKSVAEEFDEILKILADVMVLDDESFITINQTKIS
                     VQVVKERFRKLNSGHLEYLVGVLRENEYRIRNIRAFVLTAAYNAPSGIDAYYSALVSY
                     DMRGGY"
     gene            complement(58049..58330)
                     /locus_tag="KPH29_000056"
     CDS             complement(58049..58330)
                     /locus_tag="KPH29_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1465702.1"
                     /translation="MRWILKIILFPISIALSILIAFLSFLLGIGTAILYLLMMFCIFG
                     AIASFLQKEVTIGIEALILGFLLSPYGIPMVGTAVIAVLTGVKEAIRSI"
     gene            complement(58349..59983)
                     /gene="rlmD"
                     /locus_tag="KPH29_000057"
     CDS             complement(58349..59983)
                     /gene="rlmD"
                     /locus_tag="KPH29_000057"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBG1465703.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIHDKVKVR
                     INKVKKNYAEGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHVETVALLS
                     KLDVDKHIDVEIKLDELDLTSAESKATYAQIKEYVLEKFGLKVSTLYIAQIKKKCGIE
                     LREHYNKSKKDNQAIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(60158..61918)
                     /gene="pyk"
                     /locus_tag="KPH29_000058"
     CDS             complement(60158..61918)
                     /gene="pyk"
                     /locus_tag="KPH29_000058"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBG1465704.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEILTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSATDITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            complement(61970..62929)
                     /gene="pfkA"
                     /locus_tag="KPH29_000059"
     CDS             complement(61970..62929)
                     /gene="pfkA"
                     /locus_tag="KPH29_000059"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBG1465705.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            complement(63138..66707)
                     /locus_tag="KPH29_000060"
     CDS             complement(63138..66707)
                     /locus_tag="KPH29_000060"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBG1465706.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            complement(66864..67811)
                     /gene="whiA"
                     /locus_tag="KPH29_000061"
     CDS             complement(66864..67811)
                     /gene="whiA"
                     /locus_tag="KPH29_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBG1465707.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            complement(67828..68253)
                     /locus_tag="KPH29_000062"
     CDS             complement(67828..68253)
                     /locus_tag="KPH29_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG1465708.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            complement(68322..69428)
                     /locus_tag="KPH29_000063"
     CDS             complement(68322..69428)
                     /locus_tag="KPH29_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBG1465709.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            complement(69440..70297)
                     /gene="rapZ"
                     /locus_tag="KPH29_000064"
     CDS             complement(69440..70297)
                     /gene="rapZ"
                     /locus_tag="KPH29_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBG1465710.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIDEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            complement(70328..71041)
                     /locus_tag="KPH29_000065"
     CDS             complement(70328..71041)
                     /locus_tag="KPH29_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBG1465711.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYNDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFKLA
                     MKTISDSNLDISLVENIII"
     gene            complement(71045..71959)
                     /gene="murB"
                     /locus_tag="KPH29_000066"
     CDS             complement(71045..71959)
                     /gene="murB"
                     /locus_tag="KPH29_000066"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBG1465712.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKEESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            complement(72232..73002)
                     /locus_tag="KPH29_000067"
     CDS             complement(72232..73002)
                     /locus_tag="KPH29_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBG1465713.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
     gene            73320..74801
                     /gene="cls"
                     /locus_tag="KPH29_000068"
     CDS             73320..74801
                     /gene="cls"
                     /locus_tag="KPH29_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBG1465714.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKIKESISRLLSAVL"
     gene            75119..75616
                     /locus_tag="KPH29_000069"
     CDS             75119..75616
                     /locus_tag="KPH29_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HBG1465715.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLTTKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
     gene            75633..77519
                     /locus_tag="KPH29_000070"
     CDS             75633..77519
                     /locus_tag="KPH29_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBG1465716.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            77542..79323
                     /locus_tag="KPH29_000071"
     CDS             77542..79323
                     /locus_tag="KPH29_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBG1465717.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRICMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(79463..81259)
                     /locus_tag="KPH29_000072"
     CDS             complement(79463..81259)
                     /locus_tag="KPH29_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HBG1465718.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGFAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENVILE
                     RQRAIKELLSKDDFSIHIQILSNIIGKEQKNNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLSPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVISHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(81473..82402)
                     /locus_tag="KPH29_000073"
     CDS             complement(81473..82402)
                     /locus_tag="KPH29_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBG1465719.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
     gene            complement(82402..84219)
                     /gene="uvrC"
                     /locus_tag="KPH29_000074"
     CDS             complement(82402..84219)
                     /gene="uvrC"
                     /locus_tag="KPH29_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBG1465720.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVEAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            complement(84478..87303)
                     /gene="uvrA"
                     /locus_tag="KPH29_000075"
     CDS             complement(84478..87303)
                     /gene="uvrA"
                     /locus_tag="KPH29_000075"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG1465721.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            complement(87322..89292)
                     /gene="uvrB"
                     /locus_tag="KPH29_000076"
     CDS             complement(87322..89292)
                     /gene="uvrB"
                     /locus_tag="KPH29_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBG1465722.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            complement(89479..90741)
                     /locus_tag="KPH29_000077"
     CDS             complement(89479..90741)
                     /locus_tag="KPH29_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBG1465723.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKVKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEMVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGVVIEEGD
                     LRAHAATILKMFDLKLDGVEKEAFDFIM"
     gene            complement(90804..92108)
                     /locus_tag="KPH29_000078"
     CDS             complement(90804..92108)
                     /locus_tag="KPH29_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG1465724.1"
                     /translation="MKLKRKLATGLAITLLLGSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INRDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            complement(92139..92999)
                     /locus_tag="KPH29_000079"
     CDS             complement(92139..92999)
                     /locus_tag="KPH29_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBG1465725.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSMEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLILQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYIPLIIPFMTS
                     SFGIFLLRQNFMQISDETIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            complement(93043..93912)
                     /locus_tag="KPH29_000080"
     CDS             complement(93043..93912)
                     /locus_tag="KPH29_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBG1465726.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            complement(93896..94966)
                     /locus_tag="KPH29_000081"
     CDS             complement(93896..94966)
                     /locus_tag="KPH29_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1465727.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGKLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
     gene            complement(95421..95537)
                     /gene="rrf"
                     /locus_tag="KPH29_000082"
     rRNA            complement(95421..95537)
                     /gene="rrf"
                     /locus_tag="KPH29_000082"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT        27590 a        19368 c        13926 g        34730 t
ORIGIN      
        1 tatgaaaact atgtagtttt aattataaaa aaagatgaac aaaaattata cagtttaata
       61 tggaatcagt aggtacatat gtattattaa gaattataaa taataaaaat aaattttagt
      121 caatctacaa aaacctttta aattgtctgg aaatcacctc ctcatttgtt ataaaaacat
      181 acaacaaatg aagaggtgat tttatggttt ttaaaaaaat aattataagt ggatatgtgc
      241 tttcacatgt ttatcaacat ttgtaatgcc tatatttgct aatgaattat aatataaaaa
      301 tattaaataa tattcaacta tagattatgt aaattatcct atgaaaaaaa taagttgtat
      361 aaaatctggc aactgtagat tgacgatttc ttttaaaatt acaattataa taagttttat
      421 agaattaaaa ataaaacttt agagattttt gataacattg tagactaaat ttgccaccga
      481 aattacttta cagtggcaaa tcggtggcaa atacaatata ttattagtat ataatctata
      541 taaacattac tattgcttga ttatcttagc tacagtttca acttgctcat cattttccaa
      601 acttatatta agatcttcat caatgatagg tagcttaaaa gtaatggatt ttagccattg
      661 tccattaggc tgcttttctt cataaatttg aatttctgaa atgagtgctt ctattaactg
      721 cctgcgttca acatcgttca tgactttata aagcttgtca aaataaatca atactttata
      781 tatgttatca ccagtaagtt tctcaacctc tatcgtttct ttttttgctt tagcttctat
      841 taactgactt tctaattcct caattttatc gtacatacga taaagcctat catctaaatc
      901 attttttctt ctcttataat gtttatcctc tacatctaaa ttatctattt cctcaataag
      961 cttatacttt gtagaatgac tttttcttag ttcattttgg taattatcta tctcattttc
     1021 aatagcagat gtatcaacct tcatgttaat tttttcttgt atcatagatg caaatttagg
     1081 gttgcttact aacttgatta ttacctcagc aacggcatca tctaacaatt cttctctaat
     1141 ttgtttatta taggtacatt tatgtcctct attcataagt ctatgcttac aaccatagta
     1201 atagaagtct ttatacttcg tcccatcttt tttatatttt atagatttat tcccaaacat
     1261 tccagctcca catatcggac actttactat tcctgataat aagtgtgtcc tcatgttctt
     1321 gcccttattt acatgttcat actttttagc ttgtgctttc agtttaactt gtgcagcctg
     1381 ccacaattca tctgggatta ttgcttcatg aatgccttct gctactaaat aatcgtcctg
     1441 ctctacttgt ctgtattcat tccttgtacc atgaactttt tctaaagttc ttcttccaaa
     1501 tgctattttc ccgttataca caggattctt taatattttt cttataagac ctgcgtcaaa
     1561 caatggattt ttcccatttt gtcttggtat tttccttata ccatgatttt ctaaatattt
     1621 agaaagacca ttagctccaa tacttgtatt tacatattga tcaaaaatag ttcttattgc
     1681 aattgcttct tcctcattta ttatcaattt cccatcaata agttgataac catacggagc
     1741 aaatccacca ttccatttac cctcttttgc cttttgaatg cgaccttcca ttgtttggac
     1801 acgaatgttt tctctttcta tttcagccac tgccgataaa acagaaatca tcaatttacc
     1861 cgcatcttta gatgaatcaa ttccatcttc aacgcaaata agatttactc caaaatcctg
     1921 cattatttgt aaagtcgaaa gtacatcagc agcatttctt gcaaatctcg atagtttaaa
     1981 tacaagaacg aaagacactc catcctttcc ggattttata tcttccatca tacgagtaaa
     2041 ctgaactcta ccttctatag attttccaga cttacctgca tcttcgtatt caccaacaat
     2101 ctcataatca ttatagagag caaaagcctt cattcttgat ttttgtgcct ctaaagaata
     2161 accatctatc tgtatggatg tagatactct tgtatagaga tatactttta ttttttcttt
     2221 tgacatagca ttaacctcaa tatccttatt atatatttat atatttttac caataaaggt
     2281 ttggacttta atctcaagta tattatagca cttttatttc tctttctcaa tccgtgcttt
     2341 tagctatatc aaatttattc ttatcctctg tattttttac tggagtagga tttggaagat
     2401 tctctaaatc taaaactcca gcatattttg taatcagatt tgctatcaaa tcagccaatc
     2461 cattccagtc attatccact aatacacctc ctccacttta aattctttat aaccaaatat
     2521 tatttttctt actaagtttt atgacattgg taggtgcatt ggctgctaca taggaatctc
     2581 acctccgccc cttgtttcag acgaggcagc ttaaactatt gaagtatcat tatcaccact
     2641 tacatcatcg aaataagctg ccacgcttat cttttatgta ttcctatcta tcgctcattt
     2701 tcttctaacc tcggcattat gccggtattc atttgaaccg attttcatca gcagaaaagt
     2761 cttggcgtag gtcataactc tcagtaagta gataaagtcc taccttggtc tattcagttg
     2821 tcaaagatca atatttgaaa gggactgttt tctttacatt ttcctatttg ccataagcag
     2881 gcgtatctcc tactcaagaa agtaaaggag gtcaaactcc ccttcacttt acataaggaa
     2941 aatttgcccc ctttggttac caaagtttta aagttcttcg ataaattttt tcagcttttc
     3001 aagaattttg tttcttcttt tgattaaagc cggatgtgta atacttttga gctttgctac
     3061 tgaacgaaca gtttcatcat tgaaatacaa gcgttcaata atatctcttt cttctgcatt
     3121 gagccttgtt atagcacttc ttactgcttc aatcatcatc tgtgtttcaa caatcttttc
     3181 tacatcaaca ctttcatcaa caatattatc tgtaaagtgt ccgtcatgat ccaatgctga
     3241 aaaaaagagc aagtggtttt tcctgtccac ctgctctaaa tacttctcgt gttctttttc
     3301 tcgccagtag actatatata tatcttcact gacttttacc ttttgtcctc tgatataaag
     3361 ataatactct tttgccataa agtttcctcc atctcttttt tatctgcttg tttttcagat
     3421 aaaaaaggag gacttctgca tcttggcata agaagtcctc tgaaatgaaa gaataaaaat
     3481 atttctttat atgaacttga ttgtttcaat tcgcacctga aacaatcatc tactatacat
     3541 attataaaaa agaggaatgt attgaattct aaatatttct aacattaaac gtttagatgc
     3601 ttccatattt atttcctcca ttttatcaat aatctaaata accatcatat tcatctacta
     3661 cttatataat tgagttttat taaacttttt aagtttctat tctatcaaat atttaaacag
     3721 tctctgcatc cgaaaagact aactcatata gttttactaa caatattttc attagcgtgt
     3781 tcaaatcctg atttcttgta tcaattattt tctgttcact ttcaataaga aaaaagcctt
     3841 tacttccatg gctaataata agtctctatt tttcaataac tattgcaaaa ttatatcttg
     3901 tttaagaaaa tattttaatt ttaaagataa aaaattcttc atcctgcaat gctttatatt
     3961 ctatattgta attattcaga atattactta caatatataa acctaatcca ttactatttt
     4021 ccttatttaa atcaaattta acatcaaata ttttatccat attcgaaatt ttattgttac
     4081 cgtatgaatt ctctatatat aaccaatcat taactatccc aatattaatt accccattta
     4141 catcagtata ttttaccgca ttactaatca aattagatag aataatcttt aaagctgttt
     4201 ttcctatata aacattctca tctaatatat aattattgac agttattttc ttttgatttg
     4261 ctaatatttc atactttttt aatatgtctt ctaatgtatc atttattttc aaatattctt
     4321 catcattatt caaattttct atagaatgaa ctgataatat ttgagaaata ttttttgtta
     4381 aactatcaac gatttcaata cattcattaa tataaatatc tctttcttta tattttccaa
     4441 tattgtattt catgttttcc agtattattt taagactagc aagaggagtt ttaagttcat
     4501 gggatgcacc tttaaaaaag tcatatttta acttttctaa ttttaaaatt tctttatttt
     4561 taaattccaa atcgtcaatt gttcttaata aagtagaata caaatcatta atttgttgtt
     4621 ttaattctcc aacttcatca ttagagctaa cttttaaacg cgttttttta tcaagtttca
     4681 tcattttatc agtaacaatt tttatttctt gtatattatt ttttattgat tttgcataaa
     4741 ttaaagaaat aatagcagaa aataaaattg atattaataa agaatatggt aaaaatttaa
     4801 ggcttaaatc tttcgcatct ttttgcatat cagctgtaga aacaaattgt agatttgttt
     4861 ttttaccatc attaagcttt atttctcttt cttcaattat caaagaatta ctatcacttt
     4921 ctaaattaac atttatatta tctttgattt gtatttcatt cttattattt ttttctttta
     4981 taaatgcttt tatttcacta ctcttagaat aaagctccaa agtttgttct atatatttta
     5041 tttcttttcc attcatatta ctagaaattt catttgccat attatgaatc ttttcttttc
     5101 tcgtctccaa atatgtttta ggaaagataa aaaaaactaa tgaatgaact aatataatta
     5161 ttattccaag aacagaaaat atttgtatga acatttttgg aaatatcttt aattttttca
     5221 ttttctctcc aatttatatc ctacatttct tatagtagtt atacagtcta attgtaattt
     5281 ttttctaagt tcttttatat atacatctat tactcgatca taaggagttt cttcgctatc
     5341 tttccataca taatcaataa tttgcattct tgttaacact tgtccattat tatccaataa
     5401 acattttaat acttctagtt cttttgcatt tacatctatt tcttcaccat ttattttagc
     5461 tgtatagcta ttaaaattaa ccgatagatc attatattca aacttctcta aatgtccata
     5521 attcttctta attaaagaat ctattctagc ctttaagacc ggcaatgaaa atggcttttc
     5581 tacatatccg tctactaaat tagtaaatgc atcaatttta tattcttcat cactaaatgc
     5641 agtcaagatt agaattggta aattgctttt ctttctaatt tctttcaaca cttctaaacc
     5701 atttataaaa ggtatctgaa tgtctaagat aaccaaattt atatcgctat taaattttga
     5761 caaggcttct cttccatctt tagcttgaat aacagtatat ccaaattctg aaagatattc
     5821 acttattccc tctcttatca tattatcatc ttcaacagtt aatattttca tatatacctc
     5881 cacttaagcc tctacactta tattatacag catattattt gatatttaaa agccaaaaaa
     5941 tttttggtgc taaaaagcac cgaaaatttt aattattgtc aattaataat tcttttggat
     6001 tccttcttaa tatattgaat gaagaaataa tcaatgatac aagaagtact aaactcataa
     6061 atataactac ataaattatg aatttaggta atacatttat atctaaactt gataatgtct
     6121 tgttaaatcc ttcagcttct gccccaccac ctaattggct agatgcagat tgtctagcta
     6181 tttgtttagc tatatcacca gtaaccttat tcaatatatt attcccaagc ttatcagctg
     6241 tatattgagc taagaaataa gaaccaacga atgcggggat tgatataaat accatctcaa
     6301 ttaggaattg accaaaaatt tctaattttg atatacctaa tgatagtaat actgctattt
     6361 ctttctttct agcattcatc cataagaata ataataatga aactacaact cctgcaaata
     6421 ttaatgagcc aacaaataat ttatttgaaa ttgagtagat acctgatata gattgttgta
     6481 atgcagggta atttgatgaa cttttaatca aattatattc tctccaattt atatctaatt
     6541 tcccaagatc ttttattaca ctatcaagat tcttatcacc ttttacaaag aatgttgcat
     6601 cttggtatac agctgtatct tctgtattac cataaacttt agcagctgta tgaacatcag
     6661 tgattaatgt attttcgtat agttcttgtg ctgcacttac tccaccgcta ttatgcccat
     6721 cgaatagacc tttaatttct acttctactg tttcatctgc acctttctca ttatcagcat
     6781 caaataaatt agattttatt tttatcttat ctccaacttt aagattattt ttttcagcta
     6841 aatctttatg cattaagatt ttatttttat cttcattttc taaatgcttt ccttctacta
     6901 atttatatgc ttctgataca aattttgttt cttttgatga gtcgttaacc ccagttaaca
     6961 taactgttct tttaaaattc ttcgctcttt caggtgattg attcgcaaga gtctcttgag
     7021 tttcaataat atcataatca actaaatctg caacactgtt tattcttttt acataggagt
     7081 ctatactatc tgtttgaaat atctttttaa tatcttcacc ttttacattt cccccacctc
     7141 taggtgttcc tggatttact tgtctattta tctccataga aaaactattt gttatattag
     7201 caaatgtttc ttttgaagct ctgtccgtag catctttaat agataaacta atgagactta
     7261 aagttgacat agataaaata actaatataa taattaatga ttttaagctt tttctagtta
     7321 cataagcaaa tgcatttttt atcattattc aacctccaaa ttcattttat ttactttttt
     7381 caacttttta ccacttagtt ctaaaatgat atccgcagaa tctgctactt ccttactatg
     7441 tgttacaact attacacatt tatttctatc tttagctaat gtctttaata tattaattat
     7501 ttctccagca gtaacactgt ctagattacc agtaggctca tcagctagta ttattggagc
     7561 atctgatacc aatgctctag caatagctac tctttgttgc tgtccaccag ataatttcat
     7621 aacatttctt tttatttgtt ttttatctaa acctagttca aacaagatac tttcatctgc
     7681 tgatttattt actaatctaa tattttcaat tggtgataaa taatctatta aattatagtt
     7741 ttgaaatact aaagatatat tattttttct atgattacta tatcctttct tttgtatatc
     7801 ttcattctta aacaatattt ttcctgtttg aggtttatca agtccagcaa gtaaggaaag
     7861 aagtgtagat tttcctgttc ctgactttcc tactatcgca taaaacttcc caagttcaaa
     7921 tttttgattt actcctgaca aaactttttc tttagaattt gcataactat atgctacatt
     7981 ctttatttct aatatatcca ttattttctc ctaacttatt tttgataata tttctttagg
     8041 cttcttgatt aatattaatg aagaagcaaa tacaactgat aaaacaataa tacttattaa
     8101 tattaaataa ctttgtccaa gtgttgttat atttaacata aaactactat tatttattaa
     8161 acttccaccg gaaatcattg agtcctctga gttaatgaat ccatctacaa ttacttttag
     8221 taatacattt cctaaaaata aggaagatat tatacttggt atcgatatga atattaactc
     8281 gaatataaat tgcattataa tttgtatctt agatgttcca atagataaaa atatacctat
     8341 ttcataaatt ctttctctta accatagaat caagattaat gaaagaacaa ccatcccacc
     8401 taacataata gaataagtca ttatttttat tatatgtttt attccactca ctgactctaa
     8461 agactcttca aacgcattag aatctttttc aacaaaatac tttgactcat caattttaag
     8521 ctctttcaat ttgtttaagg ctaagtctgt agattctgca ctaccagaat acattaaaat
     8581 tttatttgcg attttattat tttctgattt atttaatatc tcttggcttg ttgaataatc
     8641 tacaaacacc atattttcac taaaatcaga agataatcct gtatatgttt cctgtttttt
     8701 accagaaaag atccctataa ttttaaattt atgacttttt atttttccac ttttttcaat
     8761 atctagtaat tcaagatcaa cttcatcacc taattttaga ttgttttgtt tagcaaattc
     8821 ttcatgaaca ataattgaat tcttatcatt ttctcctata tttttgcctt ctttaattgt
     8881 aaatactcca ctactaaata aaatatttct tttagtatta tttgtagctt cgagtgaaac
     8941 aacattctta aattcgtcag ataaatcttc tctatttatt ctttgttctc cgctaactac
     9001 tttagcatct tttagttttg ctaatccatc atattgaatt attttttctt caatttcttt
     9061 taatttttca atatctttaa attgattaac attaaaatat ttaccatctt tttttgttat
     9121 tgatattgaa gaattagaac tttcatataa agccttttct atttcattgc ttgatttcat
     9181 tattgttaaa caagaataca agcaagaaag aactatagtt aatatgataa aaattattaa
     9241 agttctattt ctctttcttg taatatatgc tatagcattt tttatcactt tcctaactac
     9301 tcctctctat tttcttactc cataactaat tgcatccact ggacaagcat ttttacatcg
     9361 tccacatctg atacactcca gatgattgca attctcaact ggatctatat tcatttggca
     9421 aactttttta cattttccac aattaataca cttatcctta tccaatctat atctaaatat
     9481 tgaaataggg ttaaaaattg agtaaatagc tccaagtgga catatatatt tacaaaaagg
     9541 tctatatata attatagaaa gtaacaaagt aactattaga atgattcctt tccaataata
     9601 caagaaacct atcgcatttc tcatactttt atttaataaa acaagtggta atcctccttc
     9661 cagcatacca atcggacaaa taagtttaca aaaatacgga gaaccctgac ctaaaatatc
     9721 aaccaaaaat aaaggtaata ttattacaaa tacaagtaat attatgtatt tcaattttct
     9781 caaatgttta tcaaacttaa atgtttcaac ttttttaaaa aagggaatct tatgtagcaa
     9841 atcttgaatc aatccaaatg gacacagcca tccacaaaca aatcttccca ccaatgctcc
     9901 tataaaaaac aaaatcccaa ctatataata actaatctta tattttgaat ttccaattac
     9961 tgcctgtagt gatcctattg gacaagaacc ttttgctccc gggcatgaat aacaattcat
    10021 acctggaaca caaattttct ttaaattccc accgtagatt ttaccagtct taaacccttc
    10081 aaagtaagaa ttagtaatta aaaaccaaac ggattgaaat aaatgcctaa aacgatctct
    10141 tattttatat ttatcttttt ttgtactacc atttataata tttttaattc tatccaattc
    10201 caatacactc catacaaatt cttatcgcct tatccaagac aactttagtt tctccattac
    10261 tatgaccata gtaaatcatc gccatactaa taaagataat taataaagac aaggcacggc
    10321 tttttaaaaa cttattaacc atataccaat caccgcttta cttttattta ttttttattt
    10381 tttccaattc tttttcaatt acctgcttcc attcctttga actctttgcc ccagaataag
    10441 tatcacctac aatatttcca ttactatcaa caaagaaagt ctctggcata gcctgtatac
    10501 cattcaatct accattaaag tttgtcttat ctggcattaa gaaaggatat gaagcctttg
    10561 tcttttcatg tattaacttt gacttttcaa ttgcttcctt gttttcaccg ttatcatcaa
    10621 cagtatctgt aacaactcca acgatattta cacccttgct tttcatttca ttctgaacct
    10681 caaccaaatc aggaatttcc tttacacaag ctgtacacca agttgcaaat acatttacca
    10741 tagtaagatc atacttctca aaatctttac ttgtaaaatc cttgccattt atgtcctttg
    10801 ttgatagttt acgtaaatct ttaaccgatt ctttgttaaa tgcttctgta ttttctaaat
    10861 cagttttttc tgatagcaca aaaccatttt taggacgttc ttttttatct ataatttgaa
    10921 tctcagttct ttttaattca tccagaagat tactttcagc tccgctattt gtgcttaaat
    10981 agcaatcgta ttttccatca cttgaaactc ctattttagt atgagtatca cattttgtaa
    11041 ttttagatat tttttcttct gatgtatttt tttcgaatat accaatagtc ccaattcttt
    11101 caagctcatt ttgccaattt ttatatccat cgcccatctt ttctataact gcattctttt
    11161 gttcttctgt cattttttca aatgtcaaaa tagcatactt tagttcttta tctataggac
    11221 tttgatcatc taacatcgct atctttttat catccatata ctttttaaat ttgtctgaaa
    11281 gtttaaattt aagacctaaa tattcataaa catattcttt ttttgttttt aaagtataat
    11341 ctgatggctt aaatgtttca tttttatcct tctcattagt tgacatacct gcctcttcag
    11401 aacttataga ttgtccttct ttaattttat tgctttcatt acttttttta cacccaactg
    11461 tacttaagct caaacataac gtaagtaata ggcatgctcc tcttgatact atttttttca
    11521 taaatttttc ctcctttatt cctcctttat ttttacacaa tagcagttta taactacttt
    11581 caataatatt ctacacacct tttatgaaac gttgatgaaa tataattttt tatttttcca
    11641 aactatggta taataatata aatattatgc ttaaagttgt tcttacacga ttagtatatg
    11701 atcgattagt atatgagcaa gcacagtaag tgtacggaca cttacgaaaa aattgatgaa
    11761 gcagccctct gggatatgct tcttttttta tcaattttta acttgttagg gtatacccta
    11821 agaccccgaa atacattcaa aggaggattt acctatggca aatagaatac gaaatgaaag
    11881 actggaaatt aaactaactg aagaagaaaa ggctcttttt gaggagaaac gaaagctcgc
    11941 aaggtgcaga aatatgagcc actttatccg caaatgcgtt ttggaaaagg aaatatatca
    12001 agtggattta gaaccgttta gagatttaca gggcttactg tccaatgcta caagcaacat
    12061 caatcagatt gcaaagcgtg taaattccac cggcataatc tataaggacg atataaacga
    12121 tatgaaaaag cagattgaat atttctcaaa agagttgtgg caaatacatt ctctgcttct
    12181 taacagaact tctggaggtg attaaatctt atggctataa caaaaataca tcctataaaa
    12241 tcaaccctta atcttgccat ttcatatatt gttaatggaa aaaaaacaga tgagcaaatc
    12301 ttagtaagca ctcataaatg ccatcaagaa actgctcata cccagttttt aagaacacga
    12361 aatgatgttg gaacaaacgg aactgttctt gccagacacc ttattcaatc ctttttaccc
    12421 ggagaagcaa gtcctaaaat ggcacatcaa atcggtcttg aactgtgtaa aaagatatta
    12481 aaggacgagt acgaatttgt cttatctacc cacatagata aagggcatat ccacaaccat
    12541 atcatcttca ataatgtgaa tatggtaaca ggcaagtgct atcaatccaa caagaaaagc
    12601 taccacaaaa tcaggtatca aagcgataag ctatgcaaag aaaacaacct ttctgtgatt
    12661 gatgagtttt acgaaagcta taagagaaag tacaaaacaa atggcaagtc atggtatgaa
    12721 aatgagcaat ctaagagagg tacttcttgg aaaagcagac ttcaatttga cattgatcgt
    12781 cttataaaac agtctaagta ttgggaagaa tttctaaaaa aaatggcgga gcttggttat
    12841 gaaataaagt atgggaaaca catagccttt aaaccaaaag ataagacaag atttacaaga
    12901 gctaaaacga ttggtgagga ctatacagaa gaaagattaa aagagcgtat tacagaaaat
    12961 caatctatta caactccacc tgtcaaaaga cgaatcggaa atatcatcaa tatgaatgcc
    13021 aatgccaaag tgaaagagag caaaggctat gaatattggg caaccaaaca taacttaaat
    13081 acaatggctg agtcagttat ttttatcaga gaacatggca ttaactccat taagcagctt
    13141 gataaataca ttcaaaaaag tgctgaagaa agacaagact tacaagataa aatgaaagaa
    13201 attgacaagg atatgcagct actttccgat acaatggagc aggttcatac tgttaaaaaa
    13261 tatcgagcct actacaaaga atataaagca aacccttccg ataaagcatt ttttgaggag
    13321 cataaggctg aaattacaca ctatgaaata gctctttcaa agctaaaaaa atcctattca
    13381 aagctgccga attcaaagga tattttagat aaacttaata aactgcaaga aaaaaagaat
    13441 acccttattc aagagtattc ttctacaaaa tctactatgg acgaacttta tcaaatacga
    13501 aagaactatg gaatttacat gggtaaggag atggaacgat aactctaata ttatatagct
    13561 ttcttatttt tataatatta aattgaaatg cctatcaatg atgcaagcat ttttatacct
    13621 aaagctaaat caggaattaa agctacggaa gtagtgataa aatgcatact ttttaaacaa
    13681 gtagtcaata cttctttctt cggatgcttc tgcttaattt cattttttat attatctaaa
    13741 ttctgattaa gtattgattt ggagttatca tcctccatac ctttaattaa gtcttctaca
    13801 ttgttaattg ccttgattac ttcttcagta ttaaatttat tttcttggtc agcgttaatc
    13861 gtgcttccgt gttccgcaat attgacttga actccagaac tattaacatt gatattatat
    13921 agagcattat tttcatcgta ccccatatcg gtagaaatat ctttcatata attttcaacc
    13981 tcaagtacaa acggatagac cattctatcg ccaaacgcct ttgccatatc ttgatattta
    14041 ttactgccag cataatacca gccaaaagta tatgtaggta gattatttcc tgttataaag
    14101 gaaaatgctc tatacagtag atacgtcctt ttattactat cattgccaag attcaaaact
    14161 ttacgtccat aagaattact tatcgtattt aataagtcat ctaagccatc aacatcatat
    14221 tctaaactat gcaaatactc ttttatcaac aatgtttgct ctatataatt tatcatttct
    14281 tccaaatttg cattctgttc tccataatat gaattcataa catttgaagc taatatccta
    14341 aattttctac tgatttttct aagttctttt ttactaatct ccatctatca tctctccaca
    14401 aaacgggcaa taactacctt ctaatttttt tatactactt attctagcgg ttttgccaca
    14461 acacaaatat ttcttttctt cataatctcc agtttttctt cctatcggaa gttctgcttc
    14521 ctttttaatc tcttttccac gtttaaactt tatactttta ctattcctaa aacttttttc
    14581 aagattcttt gaaaaatcat ttagcatttc agcaacttga ttttcaacta ttcgttctgc
    14641 taattcataa atatcatcgt ctaaataatt ctcgtgtgtt aaaccacaat taggacacca
    14701 aatatctatt gtgctttcag cctcaatatc ttccaccgtt aacataaatt tttcactaca
    14761 acttggacat tttaattgaa catatccctc ttcatccgct ggaacagaaa tttcaaattt
    14821 tatatcatta gccatttaaa tttcatctcc tcacaataaa agttataatt tcaccactgg
    14881 tagactacaa ggtcgtgcat ctttacgtac aataacaaca ctattctcag cacgtgtaac
    14941 agcaacatat aatctagcct tactttgttc ttttagagat gtgttattgt taataatcca
    15001 atcccacatc ggttgagtcg gatatataag tactcgttgg aattccaagc cttttgattc
    15061 tccaaaatta taatatggat attcattatt gacggatgtc cttttatttt ctctcaactg
    15121 cattgcatta aatttctgca aatacttctc tacatctgct ttatcaatta tcataacacc
    15181 atctgattca tcttcagctc tacgcacaaa atttgtagat gttctctctg gatacagact
    15241 attcgcaaat ttacatataa tatcattgtt tctataagta caattaagtg tcgtttcatc
    15301 aattcttaca tctaattttc tgcactcatt ttttaaaaac tcatctattt tcccacaact
    15361 atatttttta tatttttttg atgagtgagt tgaatatgtg cattgtctag gatccccgac
    15421 cattatcact tcaattcttg aagctattaa acactttaat atttccaaat cataccccac
    15481 catatcttgg acttcatcaa catatataca gtcatacatt ccactgattc tttcaaaaac
    15541 ttttccatta catttatcat ttaactctac aacacactct gataatttat cagtatatac
    15601 cctgttatct ttggcaaaat atttgttgga tgttttcttt acgtacgtat tgttctgtcc
    15661 atttacaaat ataattcctt caattcgttc cttaaatcta cttccttgat aaggtctaat
    15721 accatctttt aacaaaaaag aaaaccatgt agaaactgta atattattag gaattacacc
    15781 aactttattc ttaattcggc taattatttg ttgctcattt gtaatagtat atgttaggat
    15841 tagtattttt ttatgagtat taagagaatc ctctataatt tttcttgttt ttccagatcc
    15901 agcagcagct atatataatt tcccactcat attagaatac cctcctttat atattgtgga
    15961 aattctatag ctttatcact ttcaaaaatc tttaaagcaa cttctgtttt atgccccttc
    16021 atatatttaa gtaaatcatc tggatcttca tatgatttat tcaatatctc gtttaaaata
    16081 gacatggaat tcgatctata tatacacggt tccaaagtat tatagttgta attttccaaa
    16141 gaaccattat attcatgggt actatcatca aaatatatat ttatattctc acaacaatta
    16201 tctcctaaat aatcgctata ttttctattt agtgcatcaa tatttccatc attatcggtt
    16261 atcactgaca cacgtaattc tagcaatttt gcgatttcca aaaatcttaa aaaagaaatt
    16321 ccaaccgata ttatatctat tccatcatta ataggcaatt taccattatg agagtccata
    16381 tatgctcttt gaacaattag ttcgtccgaa tcaccttcga ctaaaattgc tttatttgat
    16441 aataataatc gtaacgtatc ataacctgca attcttttaa aaaataaatt tgtttcattt
    16501 gataaatcac ctagccgtag tgtttttcct cgatttagta aaattaaatt ttcaagtcct
    16561 aatttatttg atacaaaact actatgcgta gtaactatta tctgtttttc ttttactgac
    16621 tcatttactt tatctaggag aatatttaag tttgaatatg acaaatggca ttcaggttct
    16681 tctaaaagca caatctgctt attttctatt ttatgatcac ttaatgccaa ttgtgtttta
    16741 acaatacatt gtgtaccttt accaatgtta ttatatggta tttcatttaa ttgggttacc
    16801 aagctgcttt cccatgaagt tttcgtaccg aaatctacag aaattgtcaa ttcgttattt
    16861 gatgtttccg ttaattttct atttatatta gcaatattct catctctaga aaattgttct
    16921 ttcataagcc tatgcgcttg cgatatatta ataatttcat tatcatctaa cagattcttt
    16981 actattctcg atatgtatat atctgaccca ctggtaggag tatagcttgt cgtatcaatc
    17041 attgcagact taattggaat gctccttgca gttatatttt ctctggcaaa agacatccaa
    17101 cttacctcat aatattcaat tggcaacgat gttatcttgt tcttacaaag cattttatac
    17161 tcattgctat atttttcatc aaaactaatt gataaacaaa tcccaggggt gtctttagtt
    17221 ttttcagaat ttccatcacc taaaaaactt ggcatttctg catcaaaata cgcttcaata
    17281 attattttag gaggttctac attcactcct tcgttgactt ctctaatata gttttccact
    17341 atattcttat tgaaaaggta ctgagtaagt tcattcctaa tatttttccc atgataagtt
    17401 ccagttaaaa ccagattaat agcttccaaa attgtagatt tacctgttcc attattgcca
    17461 actaatatat taatcccatt ttcaaaatca actataaacc aatctgaaaa gcatttaaaa
    17521 ttctttattt ttaatttcct aattgacatt ttatccccct tctttatcta atataaaaat
    17581 catgatttta gaatataaat attgataact atataaatac agttatgagt ttcctgttta
    17641 atcacaatca taaatttttt atgctacatc ccttaatatt ttttcaagca ataatttaac
    17701 aataagtttt atgatcctct agctcctgaa aataaatcta tgactgtaaa cttagactcc
    17761 cattctcctt acatcttcaa taattacgca ctccataata tcttcaatat cacaatccaa
    17821 agcattacaa attctaagca aaacatccgt agttacattc tgtccattct tcattttgta
    17881 aaatgtactt ttactcactt ttgccttttc catcaattca ttatttgtca tatctaaatc
    17941 aatgagtttt ttgaacaatc ttttataact aaaaaccctc attcttatcc tccgttcaag
    18001 aaaactatat ctagtatatt ataccacaaa atatactttt ttcataaata tattctcttt
    18061 aatcgaactt gcataaataa aaaagagccg gtgcagtcct aagaccacac cgaccgtaaa
    18121 tttatctctc agcttctttg cttgcttctt tcttttcttt cggtttttcc ttatcttcag
    18181 cctgatattt tttgatagct ccaagtatag attctttctt ttcttcctgt cctttcatct
    18241 gttcctttgc ctttaacagt tttgaagaaa gtatcctgat attgctatgc tccttgccgt
    18301 tatcatcaat ggaagttctg atctgcccaa agagtttgac aaaatctccc tgcttaaagt
    18361 cctttggaat atcactcttt tctccataaa cggagcagtt gtgatataac ttatttccct
    18421 catcatcttt agatactacg gagaagtttg ctaccttaaa ggtttctccg ttcttatttt
    18481 ccctttcaat tacatcaact tctccaacga tatttccgac aatattgata agattatcct
    18541 tttcttcccg aacataaagc aggtctttta tctgcccctg tccccttaaa tcctcaatca
    18601 tatagtcaaa ctcctcatgc agcaaattaa ggctatcatt gtccatataa gcgtcatata
    18661 gcttatctaa agcactttca tcattgatac ccttttccat gcttataatc gctttaacaa
    18721 agtctttatg attatcactt aacatatctt caactctatc tctcattgtt ttgtaatcca
    18781 tgtttttatc tcctttcatg gtaaaaggga ggttgccctc cctttatctt aaaaattcct
    18841 gctcttttat tttttgtttt tcttctgttt tactttcttc ctgataggct cttatctgtc
    18901 ctaagataga cggcttatca tctgattttt gtgtggtttc ttctttgcta tgaagattgt
    18961 cctccacatc ataagttgac tcaaaataat cactgtcctt aaaatcacta tcatatctat
    19021 ccggaatacc gtcattatca aggtcttttg caaacggatc atagaagtta ccctcatcgt
    19081 caatatcaag tcccgttgct actcttaaat cttcagaatc aacataaacc aaatcactaa
    19141 atgtagataa ctcaatttca tttttcatat ttcttagagc ttcattttct cccttatttt
    19201 cataatcaaa gctctctgtt ttaataggcg tatcatcaat gtactgtgtc caagttttct
    19261 cctctaaatt aagctcatat tgaataccat gtctttcatc cggtgtattg gtataggcga
    19321 taccgatatg ctttaagtca ggatatagag tatgaaactc atcataactg tgattttctt
    19381 catactctct gttacagaag tcaatgattg ctctttttac atcttccaca agaggattat
    19441 catttttctt ttcttccacc tctcccatat cgagcagctt atttaactct gcaagtctta
    19501 ataccttagt tttcaactca tctgcctttt caaaaggctt ttttaattcc tctttggcat
    19561 tttcaagctg ttcttttgta cttatgagct tttcttcaag catatttagt ttctccggca
    19621 ttttctcaag ggcattatcc agtcttgtga tattaccgtc cgcacttgtt ccaagctctc
    19681 ctaaatgctt tgcaacaccg tttaagctga aattatgaga attagtaaag aagttatagc
    19741 ttacctctaa atccatattt ctatactttc ctataacctt gctttcattg atttttacct
    19801 ttgaaatagc ttcaagtaat ctttctccgg ctaatttctt atcggtaatt ttttctcccg
    19861 caatggtaat agaagtaaac ttttcttcac cttccgcttt aggctctaca ttggatatat
    19921 cttttttgac agcttctatg agcttttctg ttcttgcgat ttcttcagga tagttttttg
    19981 ccaccttatc ctctaatctg taacggttag acttatagtt tgcttcaagc attttaagtt
    20041 ttgtaacctc attatccaaa tccatctttt ctttaatctt tggatcaccc gttgccaacg
    20101 ctttaatttc ggcgtaattt aagctgcttt cgtccacatc ttctgccaca cgaacaggag
    20161 tcttactggt cataatctgt gaaatgaact tttgcttatt ctctatcgtc tgccaaaggt
    20221 aggcatcaaa ggtattctct gtaacataac gatagatatt taccttctca ttttcatttc
    20281 cctgccttac aattctaccc gcacgctgtt caagatcagc tggtctccaa ggcacatcca
    20341 aatcatgaag tgcaatcagt ttattttgca cattcgttcc ggctcccatc ttctgcgttg
    20401 aacccattaa gatacgaact tcgccttttc ttacctttgc aaagagttca tccttttgct
    20461 tatcagaatt cgcttcatgg ataaaggcga tttcttcttt cggtattccc attgctacaa
    20521 gtttttctct aatatcatca tagatattaa attctccatc tccttttgga gtagacatat
    20581 cggagaaaag tagctgtgtt gacctatttt cttttgtctt atcccaaata gcaaatacat
    20641 tttttacgca tacatttact ttactatcag ggttatcagg aagtagggga ttgattaaac
    20701 gctgatctaa ggcaagtttc ttaccgtcat tggtaatctt aagcatatta tcaacatccg
    20761 gctctacaac cctatttctg acatcatccg ctctttcaga aaggctcttt aggatttcct
    20821 tttgttcttc agaaggctca gttttaataa cttcataatt agcttcaggt gtaggaaggt
    20881 taagcatatc cgcagtctga atatctgcta cttccttgaa catactcatt agttcaggca
    20941 agttatagaa ctttgaaaat ctcgttttta ccctatatcc tgttccttca ggagatagtt
    21001 caaaggagct ttgtgtttct ccaaaagtgg aagcccaaga atcaaaatgc tctaagtgat
    21061 ttttctttaa actctcatac tgaagatacc tttgcattgt ataaagctct gtcattgagt
    21121 tacttacagg tgttcctgtg gcaaaaacta tgccttttcc tcctgtcatt tcatccatat
    21181 atctgcattt cataaacata tcggaggact taaaggcttc tgactgcccg atacctgcaa
    21241 catttctcat tttggtatag agataaaggt tcttgtaatt atgtgcctcg tcaacaaaga
    21301 gcatatcaac tcccagttcc tcaaaggtaa ttacatcgtc tttcttaaaa tcatcattta
    21361 atttctcaag ccttgtttcc agttttttct ttgtcttttc aagctgtttt actgtaaagt
    21421 tctgatttct gtcatgcttg tattcttcta catagtttac gatttcatta atctgatctt
    21481 ggatgtgctt ttcctgatat tccttactca tcgggatttt ttcaaactgc gtatgcccga
    21541 taacaaccgc atcatattcc cctgtggcaa tctttccgat aaatctcttt ctgttcttcg
    21601 gttcaaagtc ttttttatca gccaccataa tattagccga cggatatagc tgcataaatt
    21661 cacgaccgat ttgtcctgtt aagtgatttg gaacaacaaa caaagactta ctgcacattc
    21721 caagtctttt actttccatt gcggaagcta ccatctcaaa ggtctttcca ctgcctacca
    21781 catgagcaag caatgtgttt cctccataaa gacttcttgc tatggcattt ctttgatgag
    21841 gtcttaaatc aatttctgta ttcattccct caaaagaaag attgcttcca tcatattctc
    21901 tgtttcggat tgagttaaaa cgctcattat acagttttac aagacggttt cttctttcct
    21961 gatcgttaaa tatccagttc ttaaattctt cttttaatag ctcctgcttt tgtcctgcaa
    22021 gcatggtttc ttttttattt aagacagaag ttttagaacc gtccggattt ataatctggt
    22081 caaatacctt tgtttcttta agatttaggg catcttcaat cagcttatag gcatttaccc
    22141 ttgatgtacc aaaggtcatt tctgcaaggt catttccttt atccctgctt tttccctcta
    22201 cattccattc acttgtaata tttgaaaatt taaccttaat atcccatctt gcatatcccg
    22261 gagtttttaa agtttcaaag atgaattttt caatatcttt aatcggtatc caagtcgcac
    22321 caagacgcac attgatttca cttgcttcta attcctttgg aagaactttg gtaagctctg
    22381 ctttttgata ttccaaacga ttcatttcat agcttatcag ctctttttct tttccattct
    22441 caacaaagcc aagatgagga agctctcgtt ccgtttgtct taactttgca aggtagctgt
    22501 ctacaatggc aatcttatct ctaatatttc cgcttaggta ctcatccttt gttacatagc
    22561 cgtacttata tgaattactg ccgtttgcac aagcgaaagg caaatctcca tcttcaaggt
    22621 taaaggataa tggtctataa aagttttgtt cttctctgat gtttagataa atttctcctc
    22681 taagttcttc tatcaaagtt ggtctgtctt tacctgtaag gctttccata tactcaaaat
    22741 ccacatagcc tttttcagat accgataaga caagagcttc aagggaagtg tccacatggt
    22801 ctatgacttt ggcttttgta atggttctct ttgagaaaat atcccccttt gccttgacgt
    22861 tttcttcttc atcaagaatt tcaatggaag atacaagagg gaaattgcta tcctctttta
    22921 aggctctggt attgcttaaa ttatttataa agccatgctt ctttgaaaag ttgtcataga
    22981 cttcattaag cttttcttga gctttcttta cctcatcatc ggaatagtcc tctttctgct
    23041 tgtagataac atctttaagg gcagtattca gttcaagata gtccttaatc ttttccttat
    23101 tcttatccgt tatctctttc ttgatgaaaa gggagttttc tctgtaatag acttcatcat
    23161 caataacggt ataggaaaag tttttgacat catccgttgc aggaattgaa gtgatttcat
    23221 catctaaaag ctctatttct tcataagttg atcctttgga tatttcttca cttgcttttg
    23281 tcagaagctc ttttaagtcg gcattttctt tcggcaggca cgcaagtgtg tttccaaatc
    23341 ttcctgaaac ttcacgcatt gtgccgagta cctgctcagg atgatctaca aaatatttgt
    23401 tataggtaag accattttca tcttcagcaa ggtgaatcca gtcctcatct ctttctctga
    23461 tactgtccct tttctttagg aatataatat ctgaggttac ttctgttccg gctatgccct
    23521 taaaggtatc atttggaagt ctgattgccc ctaaaaactc tgctcttgct gcaagatagc
    23581 gtcttacact ttcatccttt ttatccattg ttccacttga tgtgataaag gcgataatac
    23641 cgccgtttct gactttatca atggatttcg caaagaaata atcatggata aggaagttat
    23701 ttttgttata ctccctgtca tttaccttat attctccaaa gggtatattg ccgataaccg
    23761 catcaaagaa gttattggaa aaggaggttt cttccagtcc tttaatctgc acttcacttt
    23821 cagggtatag tagttttcca attcgaccgc ttaccgagtc aagttctatg ccataaaact
    23881 tggacttatt catttcatca gggatattgc ctataaagtt tccgatgccc attgatggtt
    23941 ctaaaatgtt tccctgttta aatcccatat ccgaaagcgt cttatatatt ccgtcaatta
    24001 cggttttcgg tgtataaaag ctcgttaaag tagattctct tgcagcttcg tattctgcat
    24061 gtgataagtt ctcatttaga aaagcccttg cttctttcca ctgaccttcc ttgctttcat
    24121 caaagacttc ggaaagtccg ccccagccta cataccttgc taaaacttcc tgagctgtac
    24181 tgtcaagctc tctttgtccg ctctccactc tgttaagcat ggaaattgct tcaaggttat
    24241 tatttaacct ttcgcttgga cttaactttt cagggagcgt ttcttctgta attttaaagt
    24301 tatgagcttc cgctttcctt atttgagttt cctcagtagt ctgctctggc tgtttatatg
    24361 tcaggttttc aaataccctc tcaaggtcgc tttccagacg ataaggaatg acatcgaagc
    24421 ttgttatcat cccgccaaaa tactcggtgt tgtccttcac cgttaccgta tttaggttct
    24481 ttctcatatc atcaaagcgt gttatggtat ggtttttgtc cttgtattta acttcatcac
    24541 cgacaataaa gttcggtctt tcaagactta ttttattcag caagtcctca ttatctgtaa
    24601 agtctacaat cggaatttga tggtttccac ttcttaccgg atcaagccac aaatcatatc
    24661 ttcctgtgat ttcattttta gaaatttctc tgactttata ttcttcatga ttaaaataga
    24721 cggtatcgcc tgctttaact gcaaactctt ctaaaaggct gtcctctttt tcattcagtt
    24781 ctacttcttc tctttctttg aggtaatcaa ataaggtagc ttgaactgtg ccacgctttg
    24841 tagcttcttc tgtttcttca ggtgcttcaa gctctacttt atcttcaaag ctaagttctc
    24901 cattaaagac aaactgaagc tcctgctcat ccatttgttt tataagctct atatctttaa
    24961 gttctctaaa ggtctttgga aaatcctcta aaatgagcct ctgggcattt aattctttca
    25021 gttcctcaag gttaaaatat ccccattcag gctctattcc aagaacaagt ccaaaggcat
    25081 caccgctttc cctgtcatac tccgtcatat accaagtcca atttgaacga aaaggaataa
    25141 tatatgctgc atgaacctgc ttatccgcta aatctacatc ctcctgtgcg taaatctctg
    25201 gcactctttc aagaatttca tcaggcatta gatttccagg atcattttta gaataatatt
    25261 gcggttcttt tacttcttct atctctgccg tactttctgt ttccttttcc tcaaaataaa
    25321 gttttttaga aagcagctca tcaatatgct ttgcaacact tgtccaagta aggaacacat
    25381 cattacaatc attcttctgt aatttaaaac cctttgcatc gtgccattca tcacttccca
    25441 ttgcacctga cacaacgtga gaatgtcctc cgattccata ttcatctttt aggaaattgg
    25501 ctttttcctg caaagtatga ttttctttaa aaaacttggt gattcgttct tttcctctat
    25561 caacgccgct tcctcttgaa aggctttcaa ggacttcatc ttctgtaata aagagcttta
    25621 ccttcggaag ttctgtcaga ttggtactat attcctttcg ttgcagctca agttcttgca
    25681 acttctgata aagactatct accttgtgat aatgatacct taatacattt ctgctttctt
    25741 tgtatccttc taaaaacctg ctatattctt tgattgtttc tttcagatat tcaggatttt
    25801 ttaatgcttc agataatctt ttcgtttctt gcggaaagcc accacctctt tcaaaagagt
    25861 tgaaatatcc ttgctctatt ccttcttcgc ttaaatcgtg cgttagatac cataaggatt
    25921 ctgaaattct atccctttca tagtcctgtg cctctaaaag ttctacattt gtagcaaact
    25981 cacctctgtc aagaagttca tttatcctac ttgctgcatc actccaactt aaaatttgtg
    26041 tattatcctc tcttgccgat gttccataag ctaaatgaat acctttatcc gaataccagg
    26101 aagatacttc tctttcatca atgtaaaatc cgtttccgcc tttaaaggta tctttgaggt
    26161 attctcccaa ttcttctatg ctcttgcctt ttgaaaactc agcaataaca ggaagcctac
    26221 cgccatcatg atttcctccg ttaataagga cggtatctat atccttctca gttagcggaa
    26281 tagtaaggct tatctgtcca taagaatttt cgggtaaaga aaaagaagcc ttttcagctt
    26341 ctattatccc tttatccgta ttgtctttta aatctccact acttccttga tggtcatttc
    26401 tttgagagct gaaatcatcg ccccatactg cggattgttc tcgtcctcta tcttccaagc
    26461 tgctatcaac tttggcttct ctgctctcat aaactctact gcctgtttct gaatatccat
    26521 caggtgtcct gtcagcttct tctccttgta tagatccaaa agcatctcga agtggctctg
    26581 ctcctcgctc tgtgaaaggt aattcagcct catcactgcg taggtcggat tcggatactt
    26641 ccttataaag tccgtctgtt cctccaagct gtttagcgta ttctccctga ttttttctat
    26701 tatctcgtcc gtactctcca tttcggagaa ctcgctcgtt ttcatttctt tcctgatcat
    26761 ctcgtcgaaa tacattttct tctacctcct ctaattcttc tttaatttta ttatatcccg
    26821 cttcttttcc tattaaaact tctttttgaa gctcaagctc tttgcttttt tgaatggttt
    26881 catcaataat ctttccgcta atatccgata cactttcacc aaggctcatt aaggatatgc
    26941 tgtcaaatct ttgaaaattt tctcttaaaa gctcataatc catcggataa tctaacttaa
    27001 atcttgaagc taccgcatag cttaccgagt cccttacaaa cttggtaaat gatattctat
    27061 cctcatccgc tactctaagt tcattcatta aggtatctat tttttcatca ccgtagagtc
    27121 tgcttaaaga aaagatgttt tcaagtgtgc tttcactttc ctcatagccc tcgcttttta
    27181 tcatttcctt taagacatct tgatgggctt ctttatcaaa tctccaaaga ttgacctcgt
    27241 tgacatccct gttttttgaa actgtctggc ttatatcaaa gatatagtcc acctttttat
    27301 atgtgccgta atcctctaaa attggtatcc ctttctgccc tcgcataacc gttcggttaa
    27361 agcgttccct ccagtagtcg aacttggcac aggctgtcgc ttcaggattt ttatcatata
    27421 tacttaattg acttctaaaa tcataccttt gattgtttcc gacaactttt aggagcttta
    27481 gatattctgc ttcattatac aaaacatctt gttttattag ctccaaaatg ttatgaaaat
    27541 catttattcg catttttacc tccttctttt ttcaaacaaa aaaggcggtt agattttact
    27601 ctaatcacct tcgctatata ccaaatatta ttactctatt tcaatttttt aattgattca
    27661 ctttcagata gtatcttcat ttgttcaata gctattgcat ttaatttttc caatctttca
    27721 gattggctca tgccttcatt tataaatacc gcattaagat tctcaagatt agaaaggcag
    27781 acaagctgtg atatatccgc ataatctcta atattgcctt ttaaatccgg attttcatca
    27841 cgccactcct tagctgttat tccaaataga gaaacattaa gaatatccgc ttcacttgca
    27901 taaataaaat tgattttttc ttttgaaagt ttatccggta caagtttgtt tttaatggca
    27961 tctgtgtgaa ttctataatt gatttttgcc aaatttctct taatatccca tcctaacttc
    28021 ttttgttctt cttcctttag gcgttcaaat tccttgatta aatataattt aaagtatggc
    28081 gatacccaag aagcaaactc aaacgctata tctttatgag cataagttcc accatatctt
    28141 cctgctctcg caacgatacc gatagaatta gttttttcca cccattgctt gaccgataat
    28201 ataaaacggt taaatcctgc ttcattttta attccctcga attcggggga attaaaattc
    28261 gtgttataca tttcttccca aatacctaaa aactctattg tattcttgtt tctgagccac
    28321 ttttcaatca aagcagaacc gttctcaata tttctaacca tatccgttaa agaaatataa
    28381 tctctattgt ctatggcaat aatgctgatt tctgaacctt caacatttat tttagacata
    28441 taatcacctc tcaatcatta aattgattac tttgcattat agcattttta gagcattttt
    28501 tccacgctga aattagtgct gtcttacttg cgatatgtca aaaccgcttc ttttatctca
    28561 ttttcaatcg catctaaaaa ttctttctta gaggatttac cctgagcaat atcagataat
    28621 tccatttccc actttgctgt cgtttctgcc gacttaaagg tatctgctac aatcgttaca
    28681 aggctgattc ctttatgcgt tgcaattaga ttcttcttat ctctttcgac aaatctctta
    28741 aagattagat tttcaataat tcctgccctt gttgccggag tgccaagacc ttttctttcc
    28801 acctctacac ctttttctaa ggcatcattt cccgcaatct ccatagactt taagagcgta
    28861 tcttcagtaa agtgtttcgg aggttgggta aatttttctt taacctcttt attttcaacg
    28921 ctcaaaacat caccaacact tacatcgggc aatacagcat cttcactctt ttttgatttg
    28981 tattctttaa ggtatttgct aaagccttca tccttaatta ccttacctga acttgtaaat
    29041 tcaaaaccgt caaatgaagc tacaatcttt gttgtatttt caataagcgg atagcctaca
    29101 cttgcatgaa atttatcgga tataaggaag taaacttttg cttcacttaa aggtaattca
    29161 gaaacatctt cttttactga actgattgtc ggtatgattg catgatggtc cgtaaccttt
    29221 ttagagttaa atactacctt gatacgctct gtatcaaagt catttttacc caaaatgttg
    29281 ttgactgtac tcacaatcat atcctctgtt aaacatctgc tgtctgttct ggggtaagta
    29341 atcagtttct tttcatacag gctttgagta tagtcaagtg tctgcttggc actatatcca
    29401 aaatatttat tgcactccct ttgaagcgta gttaggtcaa aaggtaaatc aggctttgta
    29461 attttttctt tttgaataac atcagtaatt tcaatcttat cgcctactaa acttataagc
    29521 tgttcagcag caacttcatc atcaattctg tctgtggaga gtgtaaatcc attcatagaa
    29581 agctctactg tgtaatattt ttctttctga taatttgcta tctcatcatc tctttttaca
    29641 atcatggcaa gggtaggtgt ctgcactctg ccaacactgt aattttgctg atacagacaa
    29701 gagtagagcc tactaatatt cattccgact aaccaatccg caatagctct tgcctgtgcc
    29761 gattcaaaga gattatcata gtcttttccg tctgttaggt tatcaaaacc ctctttaatg
    29821 gcactatctt ccattgaaga aatccaaagg cgtttcatct tctttttaca attcacttga
    29881 ttatatacaa gtctaaaaat actttctccc tcacgccctg catcgcacgc attgataacc
    29941 gtatcaacct ccttatcatt catcagcttt ttaaggatgt taaactgctt cttggttgcc
    30001 tttgctactt catacttgta gtcgctcgga ataatcggta aatcagccat attccacttg
    30061 gcgtactttt catcataagc atccggattt gccatttgaa ttaggtgtcc aacgcaccag
    30121 ctcaccctat atccgtttcc ttcataatat ccgtctttct ttttatttgc tccaataacc
    30181 tttgcaatgg agattgcaac actcggcttt tctgcaataa ctaacttcat ctgtgttttc
    30241 ctcctgttat aaatcaaaat agggcgaaag atgttactct cccgccctaa ctttgcaccc
    30301 aaaataatat gtttttaggt gcgtttattc ttcatcttct acttcatctg catcatttat
    30361 ttcttcctcg ctttcagatt cagaaaagaa atcatcatct tcctcctcta aggcttcaag
    30421 ttctttatct tctttctttt tcacaacctt aaaatagtag cctgctccta atgcaccacc
    30481 tactacaaga agcaaaataa tataggttcc taaattactc ttttcgtcct tcttctcagg
    30541 ctttacttct tccttaacag gttcttcttt tactacttcc tgctttggtg cttctttttt
    30601 ctccaccatg ttaagcaaat catcttcaga tacttccgta agaagcatta cattctcact
    30661 atcttcatca tgattgatga ttaaatgaaa agtcttaccg tttttcgttt gaaaggtaat
    30721 aaactgcctt gcatctgccg aatactgatc cgtttctttg ttatcgctgc tatctccatg
    30781 atgaatcgga tactccttat ttgcattatc tttattctcc gtaacggatg ctctcgcctt
    30841 tgacggagat gaagctactc ccttgttggt gtttacactc ttacttgcag accccattga
    30901 tgaatcctga ctgttattcg caggagcttt tgccgtcaac ttattgggat agcgaacttc
    30961 cttttctttc tcctttactt ccgttttgcc tttaccggta tcctttgtag tattacctga
    31021 gcttaccgaa tccgatgagc ctttaccggt actacttaca gaagtctgtg gtgtttgagg
    31081 agaaatacct gaacttgtct taattccgga aataggacta atcggtgtaa ctgattgtga
    31141 tgttacagga gctttatttg tttcagttgc ctttttctca agctctttca ccttttccgt
    31201 cagcttatct atttcctttt tcatatcttc tgataagtcc ttatttccct tatctttctt
    31261 cattttttct ttcaggctat caatttcatc ttcaaggtct ttaattttat tcttttgctt
    31321 atcacttaat ttgtctttgt ccttaagttc gcaatttaac ttatcaagtt tatcctgaag
    31381 ctcttttgcc tctttttcta tttttgaaat atcgtcttta gaaagctctg tctgcgttcc
    31441 cttatcttct gtctgagtgc attcatcctg ctttggcttt tcttcttccg tctgcgtttc
    31501 cttatcctta acctccatct ttgctacctt acggataatc tcatcttttc catctccctt
    31561 tacctcataa aacagaaatt catcaatgaa tttcatatca tctgggagca ttggaagatc
    31621 tccgctatca ataagttgtc ctttctcagc ctttatcttt tcttccttaa agaccttttc
    31681 atcttcaaag atatacctta catttacttc aacttccgtt tgaacagctt cgttttttgt
    31741 tggagcttca gatttttgct cctgtgcata aacaaccgtc caaagaccta aaatacaact
    31801 aatacttaca attaccgcaa gtgctaccgt ccaaaactta ttattctttt tcaaactggt
    31861 tttcatttgt tttctccttt ttcatttctg ctttttgtct gtttaccttt gctatcaaat
    31921 cgctgattgt gatgttgttc tttctgcaaa tggcaatgat ttcttcattt tcaagctctt
    31981 cttcacgaat gaataaaggc tccaattctt catcaatcag agccttttta tcctttaact
    32041 tctttattct gttttttact gttgtaagtt cctttttcaa attgttacct cctatttctt
    32101 tacatttggc ggaaaaccga atcctacggg atggtgctta caaaatgttg ctatccaatc
    32161 atctaaggta gttactctta ttcgaccgat gttatcaatc acttttccat ctccaatgta
    32221 aatacctaca tgaccatagg taagccctgc actgcttccg ctactgctgc tttcaactgc
    32281 cacaagcatt cccactttta gctttgacct gtctgatgta aaggtatagt ttcggtacat
    32341 atcacaggca ttcccgccta tatatccaag tcctgcattt tgatagacct gtgataccca
    32401 catggcacac cagcctgcac ccggagatgg cgtaatgtat gcagcattaa caatcttctt
    32461 ttgtacttcc gtagatgcct catactcttt tccaccgccg atacctccat ttgcactgat
    32521 aaggtcagaa ttaccaaagg cttctctcat attgccttgt gcaaggaaca aggcttcata
    32581 gtgctttaga ttatcgggat agtttgcaaa gacctttcgg ataatactgt ccatctcttt
    32641 tttatgaagc gttacgatga gttttttata ctcataaggt tcttcgtggc tttcggtata
    32701 ttcattgcca tcttcatcgg tataggtttc cgtaaccgtc ctgtatctga tttcaatttc
    32761 ttccctatac tcaaggtcat acatggactc aaataattct tttaatatgg attctacttc
    32821 cgatacactc tttacctctc cgcaccttga tgtaatgtag gataaaagct catggacatt
    32881 atgaccgatg tattctgtat tatttaagat atattcatca tatccaggat aattttcttt
    32941 cacatagtca acttcacttt gaagctcacc ctccatatct gaaaatttct gattgatttc
    33001 acttaggaca tttggctttg ataaatagct tgttgtaagc acagagcttg tagagttcat
    33061 aaagcctgtc attcccgttc ctgcaaagtt aatcacaaaa gtccctaaga taatgatacc
    33121 tatgaaaata agcattagtc cttttgcttt tcggataatc atatcctttg agcttttcag
    33181 tgttccgata agtccctctt taattcgatc tctaagcctt gacttattct cacgagaaat
    33241 tgctgccttt acctgatttt tcttttgaaa tcttttatat gcacttgctc ttttatactc
    33301 atccgtcttt ttcagttcct ttttggcatc acgaaattcc aactttgact tacgctttct
    33361 gattttatag tccttatttg taaggtcata gcctctttta gctcttttct tatcagagta
    33421 cttctttatg ccatgaatga gcttggagct tgtatctgct gtcttttctc ctgcttccac
    33481 tccttgattt tcatcacttc catgagacaa gtaatctctt acagtttcac ttcctttagc
    33541 aagtcctgaa agggcagata cctttaccat gtcttttttt agctttttct tttggtcttt
    33601 tgataggctg ctataaactt ttccgctgac agcatctttt cccgcctttt tatcatcagc
    33661 cttatttccc tttggtgcat cgtccttttt ccttgtataa aggctttcag aatagttctt
    33721 cctcttgtac ctgctttttt gagtcttatg atttttaaag gaattttcta atgatttttc
    33781 tttatgggaa agattatcct ccacatcata ggtcgattca aaatagttgc tgtctctaaa
    33841 gtcattatcg tatctgtcta taatcccgtc attatcaagg tcttttccta aaggatcata
    33901 gatttttcca tccttaacct ccgtaatata atctgatctt cccgtttcaa ttgcggaatt
    33961 tacgtctccc ttatctgaag ttctatattt ttcgtttctc ttggatgttc cataagactt
    34021 ttcattatca gcacttacct tacttgtttt ttcatgcact ttatcctgaa accgttcttt
    34081 ctcatgaacg attttacctc tgtaatcatc gttatgtttt agcttacttt cttcaggtat
    34141 cgtatctgtt ttactatgaa acatctctct ttcaaggctt gccttatgcc tttccttaaa
    34201 atcctttttc agcttctttc ccataggcta cctcacttct tccggcttgg tagtcatctt
    34261 ctgatataaa attgtatctt tcggaaactt atcaatgaaa ggcacaatgg tatttccaaa
    34321 gaataagagt ccctcacctt catttgaatt agtaacatat ctgagctgtg gaagtgagat
    34381 tttaagtttt cttgcaagta tctccctatc tcctgatgct tgattaagca ttaagacaaa
    34441 gtctgtattg tcaaagatat tttctatctc cttactcata agtaggtctt tgacattctg
    34501 cgtaatacct gttggaatac ctccccattt acgaaatctt ttccaaatct ctacggaata
    34561 ggatgctgtc tgctcatctt ttaaaagcaa atggaactcg tcgatatagt accttgtagc
    34621 cttgtttcct ctgttttgtg acaccttatt ccacacctga tcctgaataa caagcatacc
    34681 gattttcttt aattgacttc caagctcctt aatatcaaaa caaaggagct tcttatttaa
    34741 atccacattt gacctgtgat taaagacatt gagagacccc gatacataga tttccatctc
    34801 tgttgccagc ttcttaccga ccttttcttc ctgccctttt aacatatcgt ataggtcttg
    34861 aagtatcggc atattgtcag gagtaggatg ttcaaaatac ttttcataga ttttaggaag
    34921 gcacctatct ataacggact tttcttctgc tgtaagacca ctaccgccta ctacaagctc
    34981 aagcatactc atgataaagt ttgccttgtc tttcagcggt gcatccccat caccgtagtt
    35041 catgtttata tcaagaggat tcaggtaatc cttcgactta ctgctgacct taatgacttc
    35101 tcccttaaac tgccttacga ggtttccata ctctccttcc ggatcgcaga taatcacatc
    35161 atcatctgtt actaaaatcg catttgccat ttctcttttg gcactaaagg acttaccgct
    35221 gcccggagtt ccgaggatta gaccgtttgg gttctttaat ttctttctat ctgccataat
    35281 caggttatgg cttagggcat ttagtccgta gtaaagacta ttacttgaat tgataaaaag
    35341 ctcctctgtg gtaaatggca taaatactgc cgttgatgag cttgtcaatc ctctatctat
    35401 ttcaatctta tttacaccaa gaggcagcac gctcactaaa ccttgttcct gagaatggtc
    35461 aagtcttttc agcttacagt tatgcttatt tgcaatggag cttatttgag caattgtgtt
    35521 atcgagcttt tgcactgtcc ttgcaaagtt cataaagacg atacttacca caaacattcg
    35581 ctcatctctt gtctgcaagt cttttaagag gctctttaca tcctcaccat aggtaattaa
    35641 gtccgatgga agaatgtcca tatcataacc gcttcttacc gccttcttat tttcctcaat
    35701 tttcatctta tcaatatcag tgttttttcg ttttaccatc ttaatggctt ccgattggtc
    35761 gattgcccta atatgaaagg aaatattgat gttatcgtca atatccaaaa actcggataa
    35821 catcctatct gaaagctcac tggcaaggat ttgaaaatga cttgttgctc cgatgaattt
    35881 gccgaactta aagtatctac tcggtgtaaa gttaaactca tcaggcacaa tatatgtctt
    35941 tgtgctttcc cttttcttta ggtctttgta ggaaaattta aatgttttat ttggatttaa
    36001 aacatcatga agtatcttta gcctttcttc tccgtttagg ctttctgctc ttactcccat
    36061 acttttaaga ctcgataata tatctatctc cagtctttca agttttgatg ttgcctgttc
    36121 taaattatcc gcttccactg taaaggttac atactttgat tttttcaatc cgttatttcc
    36181 ctttacaatc tggcttttaa gcatctctct aaattcaaag cgtatatcgt cgaaaccgtc
    36241 ctttttatcc ggtatctgaa ttgccgactt catttcttcg tttcgtccga gctgattgat
    36301 atatgaaaac tcaatgctga tacttggatc aaaagagttt aggaagtttg caaattgatt
    36361 aaaaatcaag tccctatctt catctaaggc aagctgatag ttaatgtcct gaaaggctat
    36421 acttttactg aagtgctttt catcaagctg gcatattccg ctttttaaaa gccttagata
    36481 gggaatggta tcttctaccg tatatctttt cggttctttc ttaaagataa ggtcaaggat
    36541 tcctccctta tctttttttg acttgcaatt acttttcttt aagtcccgct tttgacttct
    36601 caatgccttt tcgttttgca tgagttttat ttgctgaatt tttcttttct tgttcaaggt
    36661 aaacctcctt tctcactctc ttttgcggtt gataaaactt atgaagataa atgtacttaa
    36721 agtatttctc aaatgtcagt ccgtcttttt caaatagtgt gatgaaaaag ataggaagcg
    36781 tggatacgat aaggaatatg acagctatgt catttggcac aaacttcctc ataaataaat
    36841 agactggtat tccaaccagt cctgcaagtg tgaagcctat gagctgcctt ttcgttaggt
    36901 taaaagcaac ctttgtcttt accctattta agtctttagg gattggtaca tatgccatat
    36961 cttacctccc atcttcttta ccctttgaca gctcctcaaa gtgttcatat acagagccga
    37021 tttcttcctg aatagtcgca agctgttcgt ctgtgttctt gttatatctt tgctgtatta
    37081 ggtaaaaatc gttatggcag cgaccaatgt cctctgtttt ttcttccaat tcttccacct
    37141 tattatgaag cctgatccta tcaattatcg ccataatacc tgctccaatt actactataa
    37201 atactgtttt tcttttgttc atattaccct tcctttcttt tagtgtgcat ttaatacgct
    37261 tttggccagt gttccgctct ttaacatcat taaccctagc aaaaccgcat atccaagtat
    37321 cgtaaaggta cttgtgtgta tatctgttat ctgtattgtc ttaactaata ctgcgtatat
    37381 tccaagacaa accattaaaa agagtccttg taaacctatg gcaaacaatc ctttgatata
    37441 gtttgttcca atctgtcccc actctttgtt tcccattgtg gcaaatggaa tggctgaaat
    37501 ggatgagtaa acataaatct caaacattct tccgtaaacc acaagcataa tcacaattga
    37561 aatgacctgt attgccacct ttataagcga ggtttcaaag agtatcatga caagctctcc
    37621 aagaccttta tcctttaatc catctaccat ttttactatc tgatctccgg agataacggc
    37681 agaagtattg attacccctg ccgctttatt taccatgtgc tgtgccacat caaagaccgc
    37741 cattgaaaac tcaaatgcat gtgatactaa ccatacagca atccacatct tgatgatgta
    37801 cttgaaaaat tcaaaagtat ctgtatcgtg catattgttc ttttgcatta ccatattgat
    37861 aagctcgata caaaggactg ctgtgatgat tagccctgct atgggaataa tgacggaatc
    37921 gttaatgctt ttaataaagg caaatacatc tccgttccac cccataggag tttttcctac
    37981 atctgttgca actgcaccaa ctttatcgtt gatgtcaaga aacatggact ctaagtttgc
    38041 ttgaataccg cccagtagaa gctctttaaa gaattcttct atcttgtcga atattccaaa
    38101 catctaagct ctccttttct tattacttga gtacatttgc aagaagcgga atcagcttaa
    38161 gtccgataag gacaatgcct cctcccgcca taagctgctt gatgccctga gatttcgctc
    38221 ccggattatc attaccatat ccttccataa ggttaataac tccccatgct ccaagtccag
    38281 cacctactgc cataactaaa atctttaata catttactgc ctgtgcgaaa aattccataa
    38341 tttattcctc ctcattttct tctttcttct caatcttgtt atatgacttc actacaaagt
    38401 ttttgtaagt ctttccctct ttttcacgct tcttaaagta gccgaataca tgaatcaaat
    38461 cgcctttttc aaagtcctct gctttctctg ctttttctcc gtaggctgca caattgatgt
    38521 actccttgcc ctttccatat ttttttacaa gcgtaaagtt tacaacttct acttcttcac
    38581 cctctttttc aaaacttgag aaagtaggtt ctgccactaa gttggcgttg atgttaatca
    38641 tttcttgttt catcttttta aatttctcct tttcatttca ataaaaaaaa cgactggagt
    38701 ttttcttttc caatcgctga tactcttact atttagtttt cgtttaagtg ggatttctga
    38761 tccttatcat cttgcctcct gattttgaat aaaaaaacag caaatctttt taagacttac
    38821 tgcattcttg taataaccgt ctctctattt aattttgctt ttccttttcg cttcatatag
    38881 ctttctatgt caaacaaatt cttcttgtca taatcctcaa gcagcttata attcttgtgc
    38941 tttgtaatat cgtatttatc tgaaagaaaa ggtctgacac ctctaagctg aaatatacat
    39001 tttccaccgt ccattacagt aatctcatct tgactcatca gttctttgcc acaatgtcaa
    39061 gtgatgaatt aaaaaaagct atcaaagaaa tccttcttcg atagcagcgt attcaacatc
    39121 tattcacatt tcctgccttg cgttcttcga gctgatgaaa tataataaga aatgttgtcg
    39181 gaggtgcgaa tatggactac atgacgctca aagaggcaag cgaaaaatgg ggcgtttcgt
    39241 cccgacagat aaattattac tgcaccgatg gacgcatccc cggtgctgtc aaaatggccg
    39301 gtgtctggct gatccccaag gaggcagaga agccaaagga cagacggtat aacgaaagca
    39361 ggtgatacag tgctgcatat agcaatttgt gatgatgaaa aagactttgt tcagtatttg
    39421 accgatttac tgaaccaata cagcaaggaa acaggacggg acataaagat taccccgtac
    39481 tatgacggta tggagctgat tgaaaaatac gatccaacca tagacctgat tttccttgat
    39541 attcaaatga aggtggtgga cggacttcat gctgccgagc gtatccggca aatggacgaa
    39601 accgtgggca ttatttttct cactactctt acacagtatg ggcttgaggg atataaatac
    39661 catgcgacga actacatcat taagcccatg aaatatgccc ggctcaaatc agaaatggac
    39721 aaattcttgg agcgcagcca gaaagaagat accccctctc tggtgattac caacgatacc
    39781 ggcaaatata aggtgccgct aaaatcaatt cgatatgttg aaacatataa ccgcaatctg
    39841 atgttccata ccgagcagga aaatattatc tgctataaga gcatgaaaga agtggaacgg
    39901 gagctttgcg acaaagactt tgtgcgctgc cataccagct acattgtcaa tctgttctat
    39961 gtgaaaggca tcaagaagct ggacattgaa ttgattacag gcgagatcat tcccatcagc
    40021 cagccgaaac ggaaagaatt tatggaacgg ctgacggact attgggggga tatgctgtaa
    40081 tgtggttata tcaagtgctc gacataacag caacgctgac agaatctttg ggactgtatt
    40141 tattcagtat ttgtttttgc aaagaaccac ggtatcgtgc ttctgtaaat agatggctta
    40201 taacaggtat aagttttttg tgtgcgtata cattgacatg gttttctgaa cttggcgcac
    40261 tcaaagcgcc ttttcttctt acgatttatc ttgtcctttt gaaagtttgt tatagagatt
    40321 ctgtctatca atgtgctgtt gctgaagaac tgaattatat tgcaacttgt tctcgatatt
    40381 cgcaaggtag cgcggggaaa tatataactc gtcgcttacc tttttgcggc tctcgccgta
    40441 ttcatttctc gcggctttta tagctgcccc gaaagcctta aagtcgtaca atggtacggg
    40501 tctttttgcc attttcattc accctgttac attttacagt tcacctttct ttttggatat
    40561 gtccacacaa ttcatatcat taggttaaat acttcctgtt gtgtattgac agtagactga
    40621 ttttctgata aaataaaatt tatgtaaaag gaggcggcgt ttatgttgca tagcatgcgt
    40681 attagataag cattgaaaaa aaggattttc ccattttcaa catgggcttt tttgtcatgc
    40741 ttattttgcg gatgctatac aaaaaactaa cgacgtatag atatagtata gacatagtgc
    40801 aagctatgcc ttagttttgt tgtactgctc tgtgcatttt ttgggtaggg aaaccaccgg
    40861 ctgtgccggt ggaggtaccc cgtaaaaaag ctttagcttc aagcatcgag cgaagcgagc
    40921 tgctaacagc aaattatcag tgaagaaaga cttgcattag aacagcggct gcccgtttac
    40981 gggctgcggg gcaaatgatg cgggtgaaat aaaattcagt gcgtcttgag acgcatgccg
    41041 gtaataggcc cttatagggc caactcaagc caccggctta gccggtggtt atgacttgtg
    41101 taaaaaggac gagtgaaata taatgcttaa aaaatattgg ataaaatgtc cgatttgtaa
    41161 cggaaagacg agagttcaag tatttcataa tactgtatta aaagattttc ctcttttctg
    41221 ccctaaatgc aaattgacgc atatcattga tgtagaaaaa ttagagattg taatcaaaaa
    41281 tacagaaaaa caaactttta ttatttagaa gaaaggatat aaaaatgaaa cgtttaccta
    41341 aatatacgcc tgcggaagta cggaatgatc catacggatt tacttacaaa gaaatgtcgg
    41401 aagttattgg cgagaatgaa gcaaaagcct tatatgaaga attatataag caattaccac
    41461 gcaaaaaaaa tctatcaatg ttggtaaaaa atatttgcaa aagcagtgat actgaaaagt
    41521 atgtttacga actgaaagac aacaaataca ttgaaacggt ttttatcaag cggcgagatg
    41581 gtggaactgt ttgcgtgagc acacaagtcg gttgtcctgt tggttgtatt ttttgtgagt
    41641 ccgggcgaaa tggctttgtt cgtaatctaa catcgtcaga aatcgtacaa cagattatat
    41701 tgttgcgtcg aaaagtaaac cgtatcgttt ttatgggtat gggagagcct ttattcaatt
    41761 atgacaactt gataaaagca atccatattc tccgagatag atatgggctc aactttccaa
    41821 ccgacggcat taccatatca acagttggtc cggtcgatca attaaaaaaa ttgcgcgagg
    41881 aacatcttaa aattcagttg acaatatctt tacacgcagc aacacaatct gcaagaaatc
    41941 gtattattcc tcacatgcgc atatatgcta ttgaagatgt tgttaagcaa gccttatcct
    42001 attctgaaag gcataatcgc aaaattgtct ttgcgtattt gcttttaccg ggtataaatg
    42061 accggccctc agatgtaaga caacttgcaa aatggtttcg gggcaaaaaa gttatgatta
    42121 acgtgttaca atacaaccca acaagcaatt caagaattaa agcaccacag aaacgggaaa
    42181 tagttgcatt caaacatcaa ttagagcaag caggacttga agttactatg agagtttctc
    42241 atggcagaga gattaacgcg gcttgtggac agttagctaa cacatataat aaattcaaaa
    42301 aaaaatgatt aaaagtgcca gacgcataga cgcagagccg ataacgcatt aggtcaaaaa
    42361 acctatgtgt tatcggcttt tttatttaat attgcgcaaa agccgctgtt ccctattata
    42421 gaatggattg cgggtagtgt attaaagtcg ccctttttta aggatacggc ggtatcgggg
    42481 aggtatcgcc gtgtccattt ttatttactg taacccgcct aatgctttgc ggtcaaaaaa
    42541 agctatgctc cgcaagcggg atattccggc tatgaatgtg aactgtcaat acatttagct
    42601 actgcttaca tttcctttgc gcccgtccgc gtcttgcgga cgggcgcatt ttgctttttt
    42661 caactttttt ctgaaaagcg cactcaagag ccatctcccg aacgggtacg gagcgaaagg
    42721 ggcagagagg acaagccctt aactcccgtc ctctactcca cgcgggaagg aggtgaactc
    42781 tatggagcta tcttcttccg acaaggaaag aatacaacat cagtacgacg cattagcaaa
    42841 gaaaactttg gtcggcgaag cgaaaagcca ccgccgcact cttgcgaaac gcgcagcacg
    42901 cgaagttact ttttcggatt tgagcgaaag cgaactcgcg cagcttttca caacggacga
    42961 atacgaaagc gattatttcc gttttcaagt gtccggcttt gatgtactcg tcaaaaatga
    43021 actgcttgcc gaagccctta acgctttgcc cgaaaggaaa cgcgacatta tccttttgtc
    43081 ctacttcttg gatatgagcg acgcggaaat tggcgaactg ctgaatgttg tacgcacgac
    43141 ggttttccgg cacaggaaat ccgcgcttgc gaaaatcaaa cagtatttgg agggaaaagc
    43201 agatgatgaa taccgttagg aagtctgaaa atctgttgcc gttccctgtc atttccgcag
    43261 cggcaaacgg cgacacaacc gccatgtgcg cgatcttgaa gcactacgag ggttacatag
    43321 cgaaactttg tacccgcacg ctgaaagacg acgcgggcaa tacctattcc tatgtggacg
    43381 aggaaatgcg taacaggctg caagtgcgcc ttattacccg cacccttgct tttcatgtag
    43441 gataatcttt tagcccatgc ggggagcgtg tcccctttcc acgcttcccg ttatgggcta
    43501 tttgtcgttc cgcaaaagca tatccgctgt tgatgtgctt ttgcaggccg acaaagccta
    43561 ttgttctttg acaaagaaag cggcctttgg tgcgcagcat aacaggcaaa cgggtacatt
    43621 ccgtctgtac cgagccagtc ggcgggtacg ccatgacagc ttttccccat aggggaaaag
    43681 ccgagcgaga actaccgcgc cgtaaaacag gtttagcagc ttgtgggcga cgacatacaa
    43741 ggcggcacaa tgatactccc gcgttgaaca ccctcaaagt attgcgcggc gcacccataa
    43801 gcatgggccg gggtgaaact cccgtgaggt tgcagctaac aaccgcccgt ttttgccttt
    43861 tgacgaatat agtttatcca tacaggaaaa ggaggttttt ctaatggata aaaaacagac
    43921 aattacaacc gaacgcaaaa tagggaaaat cacctatctt gttcaagcgt tgccgagtga
    43981 aaaggcaacc gatacaatcc ataaaaagat tgagaaactc attgtaaagg atttgcagaa
    44041 aaagcccgga aatccgggat ttttagcgtc cgagtagtgc attaggcggc gggatatggt
    44101 ataatgatag caacacatta tacctgttta ttcggctgtc ggaaaggagg actaatgtta
    44161 cagtcgaata aaatcaccgc cctttactgc cgtttaagtc aagaggatat gcaagccgga
    44221 gaaagcggaa gcatacagca ccaaaaaatg atacttcaac gctatgcgga cgaacaccat
    44281 tttttgaaca caaagttttt tgtggacgac ggattttccg gcgtgagttt tgagcgcgag
    44341 gggctgcaag cgatgttgca ggaagtggaa gccggacgag tggcgacggt cattaccaaa
    44401 gacctttccc gtcttggcag aaactatctg aaaacgggcg aactcataga gattgtattt
    44461 cccgaaaacg gagtacgcta tatcgcgatc aacgacggag ttgacacagc gcgggaggat
    44521 aacgagttta cccccttgcg gaactggttt aacgagtttt acgcccgcga cacaagcaag
    44581 aaaatccgcg cagttaaaca ggcacaggcg caaaaaggcg agcgcgtcaa cggggaatat
    44641 ccatacggct atatcccaga cccgaacaac cgccaccacc ttatacccga cccggaaacc
    44701 gcgccgattg tcaaacaggt tttcgctatg tttgttagcg gcgtgcgtat gtgcgaaatc
    44761 caaaaatggc ttgcggaaaa caaagtcttg acgattggag cgttgcgcta tcagcgtaca
    44821 ggacaggcgc ggtatcagcg ggcaatgatc gccccctata cttggccgga caaaacgctc
    44881 tatgacatat tagcaaggca ggaatattta gggcatacca taaccgcgaa aactcacaag
    44941 gtatcctaca agtcgaaaaa gacccggaag aacgaagaag aacaacgcta tttcttccca
    45001 aacacccatg agccgcttgt tgacgaggaa acctttgaac ttgcgcaaaa gcggattgct
    45061 acccgccacc gcccgacaaa agcagcggag attgatattt tttccggctt gctgttttgc
    45121 gctggttgca gacataagat gtattaccaa cagggcgtaa atatcgagcc gcgcaagttt
    45181 tcctattctt gcggcgcatg gcgcaacagg gcaaggacag gcagcgagtg tacctctcat
    45241 tatatccgca aaaacgtact tcttgattta gtgctggaag atatgcggcg ggttttgcgg
    45301 tatgttaagg aacacgaaca ggactttatc tgtaaagcta ccgagtacgg cgacatggaa
    45361 gcgagaaagg cattagcgca gcagcaaaag gaacttttca aagcacaggc gcgtatgacc
    45421 gaacttgaca cgcttttccg caagctgtat gaggacaacg cattaggcag actgacagat
    45481 gaacggtttg tgtttctaac ttccggctat gtggacgaaa agaaatccct tgccgcaagg
    45541 atagacgagt tacaacagca gatcgcaacc gttaccgagc gaaaaaggga tatatcaagg
    45601 tttattcaga ttgtcgggaa atacagcgac atacaggaat tgacctatga aaacgtccat
    45661 gagtttatcg accgtatttt gatacatgaa ttagaccggg aaaccaacac ccggaaaatc
    45721 gaaatccatt atagctttgt cggacaggtt gataccgagc aggagccgac gcaagttgtc
    45781 aaccatgacc gccgcaacat ggtagatgta aaaagtatcg ctatctaacc ccagcaaaaa
    45841 aggggcctga aatgaaagag tacacgctaa ttatcaaggg agaaatggat tttatcatct
    45901 tatctccgca ggtgctttct tccctgatta ctcaaatcca taacagcccg gagcgaaaag
    45961 ttgtggtaag tattgagagc atcatgccac caaagtttac cgactatctg cttcgggtaa
    46021 tcaatagcaa ccgctttagc aatgaacggt tccgctatcg ctacatttta gaaaatcctg
    46081 ttacaaagaa agggatgtat gagattttac ggcaacagct ttcccgcacc aatacggagc
    46141 gatttccttg ttttcagacc atacaattga ccgacacttt ccagggtaat gtggagctgg
    46201 acatggagtg caacgatctg ttcttttggg cttgcaaaga tacagcagca aagtttgttt
    46261 acacgttccc ggatggacgg gaggaaacac tggtgattga atactgaatg agaaaagccc
    46321 catccctcaa tggttgagag atggggcaaa ctatttacgc tgcaatctcc gggatttccc
    46381 cgacaaagtt ataaacaatt ctgacttcct gcaccttctg accgtccacc ttcttaacct
    46441 cgccgatcag gattttgctg ataagccggt tcagtacgga ctcgtccagt tccgcaatgg
    46501 cggcatagcg ccggatttcc ttgataaagg ttcggacttc gctttcctgt gcgtccgccc
    46561 ggctctgcat caccgccaga tcgtgcaggc gtttctgatt ggcttcctgc tcctgttcca
    46621 gcgccgccgt cagcttcata aaccgctgct cggtgaggac gcctttggcc ttatcggtgt
    46681 acaggctcaa aaacatcccg tcaatttcct gattccggtc ttccagccgt ttccgttcct
    46741 tctctgtctg ggaagcgtcc accagatacc ggcgctccat ccggctgctg agccgctggt
    46801 agaacgcatc ggcgtccttc atggcctgcg ccgccaattc ctgtatatcc ttcagcacca
    46861 gattgtacaa atcccttgcc tcaatcttgt ggctggtaca ggcgttcttg cccagccggt
    46921 tgtaagtctg gcagatatag tatgccctgt caatcggctc ccgcatttcg ccggtgaagc
    46981 ggttcttccc ggttctgccg accttttcat accgcacctg catggacttc ccgcaggtgg
    47041 cgcagtagac aagcccgtgg aacaggttat agaaggggca ggcgttgcct ttcataatgg
    47101 ttggcctgcg gtcaatgatg tcctgtacct gttcccattc ctccggcgtc acaatcgctt
    47161 cgtggcagcc ctcgatgacc tcccaatcct cacgggggat aatgtcataa gtgttggagc
    47221 ggatgccttt ctggtgtgtc cggcagacaa gatgtgcgcc tttataaaag ggatttctca
    47281 ggatatggct gatccttgcg ctcccccatg aatagtagtt cacgtcacat tctgtattgc
    47341 tttttactct ggtgatgggg attttatcat ccatgagctg ctttgcgatc cgcatacagc
    47401 cccagccgtc cagtgccata tcatagattt ttcggatcac cggcgcagtc tccgggtcaa
    47461 ggatcagatg ccctttgtcc tccgggtcac gcatcaggcc aagaggcggc tgcccgccgc
    47521 agaactttcc ctgccgggaa cgggtcatgc gtcccgccag cacctttttg gaaatatccc
    47581 ggctgtacat atcgttcagg atattcttaa agggcgtgat gtccatttcc tgacgggtca
    47641 ggctgtccac accgtccgtg acggcgatat agcgtacatt gtgtttgggg aaaaagattt
    47701 cgatatagct gccagcttcg atataattcc taccgagcct cgataggtct ttcgtgatga
    47761 cgcagccgat tttccccgcc tcaatgtcag cgatcatgcg ctggaacgag gggcggttga
    47821 aattgcgacc cgtatagccg tcatccacat aatactcata ctggaacatc ccatgctttt
    47881 ctgcgaagtc ccggagcatg agtttctggt tgctgatgct catgctctca ttgtcgccgc
    47941 cgtcatccag agaaatacgg cagtagagag cggtgatttt ttcatagact tgttttttcc
    48001 tgctcatagc gttctccttc tatgaacagg gacacgatac cattatagta ccatgtcccc
    48061 gtccctgaat caaccgtttt tacgcagctt tttctgccat ttcccgctga tgacgccatt
    48121 cctcgaagtg ttcgcaggtc tggcgctcaa tgagctgctc aaaggcttcc cgcatggact
    48181 tctccccgga gaactcccgc accaccacaa aacggacttt attcttctga tttttgcttt
    48241 tttccatagg ctacctccat agagagacag gccgaacggc agcaccggca acgaagtccg
    48301 gcaacgaacc tctaaaaacc tgtaaactac cgtctggcct gcaaatctta ctttgtgaat
    48361 tttcttgaaa aatctcgttg ctttcgttgc cggagataat gataaatatt aaaatatccc
    48421 ttatttactg gctttttccg tatccagaca gggaaatccc cggcaacgaa cccggcaacc
    48481 aagtggcaac catcccggca acagaccgcc attttcaggc gatccactcc ttcggaagct
    48541 ccaactggcg gcattcttcc tcgctcagtt ccacaaatcc atcttcgttg ccgggcagtt
    48601 cgttgccgga aacgtcccgt tcccagccct tctgcctgcc gtatccggcg aacatccggg
    48661 gattggagaa tggcttccag cccgtcacca ctgtattcat gatctcgttg atattgtgaa
    48721 gctgccaccg cttcggctcg tcataggtgt ggcccaatgc ctccttaaaa agctgtttgg
    48781 aacagaccat gctgccggta taatgttcca gaaagccctg tatttgaccg gcctccgtgt
    48841 cctccggcat gaaatccttc tgcacctcca caagctgccg ctggatggac ttgctgaatt
    48901 tcatggaata cttcccgctg cggtagatgg tcatggcctc cgcccagacc tgcaacaggt
    48961 aggctctgga agcgtcctcg tcctccaaaa tgtgaacctc cgctttctcc gggtaaatca
    49021 tgatgggaag gaaacgccgg ttcccggccc ggtcaagggg caggaagtcc agcgtgttgg
    49081 atgaaccgcc gaacacgcac tgccgcagcc ggtctttggg ctgggcctca tagggtgtgc
    49141 ggtaggtttc cttctggcga ctgatgaacg agcggatttc ctctatgctt ttggcgctgc
    49201 tggtggccag catctctgac atctcaatga tccagtggcc ttgcagcttc aaaaataccc
    49261 ggtcatcgtc cagctttttc aggtcatcgg agaaccactc gtcccggatc gccagcaggc
    49321 ggaagaagct ggacttgcca gccccctgtc cgcccaccag acagagcatt tcctcgtatt
    49381 tggagccggg agaaaacacc cgccggatgg ctcccagcag gaagtgtttt aacatttcct
    49441 ctacataatc gctgacttct gcacctaaga agtgatggag acaggagcgg atgcggggcg
    49501 tcccgtccca cacaaggccg ttcagcacgt cctgtatggg atggtagcag ttctcattcg
    49561 ccacgatgga gagcgccgcc gtcattttct tctcgctggt caggccgtag ttctgctcaa
    49621 aatagagaag cagatacttc acatccgtat ccgtcagggc gctggtgttc ctgcgccagc
    49681 ccaaatcccg cacgatgtcc acccggtcag tcaggaggtt cagccggatg gcgtcccgca
    49741 ggagagggtc atgggagaac accgtccggc agttgccgat ggtgttggcg ggctggccct
    49801 tctccgtgac agaaaggcct tcccgcacct cgtcaacgct ctgctccggc gctaaagctt
    49861 cggctgcgct catgacggcc tgccgggtgg ctggcggtaa actctgatat tcgctgctca
    49921 atcttcctca cctcttttcc atactcagcg accacgcagg cccgttcctc catactgccg
    49981 gacagcagaa tatccagcag atactccgtg tacggctttt tttgcagggc ctccacaaac
    50041 agcgggttcc atgcttcctc cggcgtctgc ggggcatatt ccttctccca gcgttccagc
    50101 agatgcagat aatcgcacaa cacccggaaa catttctgtt ccgcctgccg gtagcgcagt
    50161 tcctcgctga tttttcgctt gcccggcttc ctgcggggcg ggtcatggcc tttagcgtca
    50221 aagccgaccg agaaatcctc cgccagcttc aaggccgcat ctttcttgtt cagcccgaac
    50281 agacgggcgg taaaatcaat cacatccccg tccgcctgac agccgaagca gtggaaccgg
    50341 cggtcaacct tcatgctggg atgtttgtca tcatggaagg gacagcaggt catcccgttc
    50401 ctgccgagct gtatcccata agattccgca gcctgccttg tggggacagc ctgcttcacc
    50461 gcttcaaaca cattcgattt tctcacctcc agacagagaa aaaggcatcc ggttttcaca
    50521 gaaaatcaga tgccttacaa ctctatatcg ttctttttgg tcggggcgat tcgcccctca
    50581 cgttcccaac gctcccggtt atcccggtca gcattaattt ttgcctttgc cagaatatcc
    50641 ataatggatt ctttggcttt cttcttgacc tgctccttcc cggcttgctt gacttccggc
    50701 tgcatcagcg tccgctggat tttcgccaga gcgtgctgca tggcgttttt gattttggta
    50761 atgaccccat ccagacgctc agcggcatac tcacgttcct tctttggagc tttgcgctcc
    50821 ggcgaaagca cccatttttt cgattcctcc accagacgaa tatcttcctt gtgcgtctcc
    50881 tgccggacgg tatcggtcac cacctccacc gccttgtcat aggccgcatc ggaaacatca
    50941 tctaacagtg tctccacgtc ctcaattttg agcgtcagtt cttccagctt ctgctcctgc
    51001 gccgccagtt gctccttctg tttcatcagg atataatcct gtttttccag atagtcccgc
    51061 ccgccgtagg acggctcctg atccagatgt agcccatgcc gtctggcgat gtcaaacaga
    51121 atcgtccggc aaaccgcatc aaaggtctgc ttgcggttgt tgttccggcc tttgggcttc
    51181 tcagggtttg ggagagggat gcccagttct tccagcgcct tttcctgctg gggacacagt
    51241 tccccgtagc ggttctcaca gtcaaagacg tgccgctcat ggatgtgggg cgtcccctcg
    51301 tccagatgga gcgcccagtc caggatgtgg atatgggaac cgaagcggcg ctcaaattcc
    51361 tcataaaact cattgacgat ggaaaaaagc gtgtccggcg ggacggattc tcccatcgtg
    51421 ccgatctggt agatgctttc ctccgggcag gtcttgttgt ttttcagcaa gtcctccacg
    51481 gtgcggttgc gctccgtgtg ccgggttttc tcattccggg cgttctgggc ctccacatgg
    51541 ccgccgtaat gctcgttgta gtacatccgc tcgatttcct caaagctgaa atccggctgc
    51601 tccgggttct cccggaactc gtgtgtcgta aagccccggt agcagtccca gtagacattt
    51661 tgccttgccc gttctgcgtc aatgtgttcg ctgttctcca catcgaaccg gcggtcattg
    51721 tggcggggat tataggtgcc atgcttgccg gagcgcccat tgtgtcgtgt cagtttcaaa
    51781 cagatttcct ccttcctgcg aagatgttct ggggccgggg aaggggcggc gaagccgcaa
    51841 atcctgcgaa ttttgcgtca ggtaataccc agtacgagtt gacgcaggca tcaactctcc
    51901 ctgggcaagg cgtttcaccc ttgacccgat aatggctgcc gcccttaacc tgccaatggg
    51961 cgttgcccct tgaccccagc agtgcgctgc cgcccctgca ccccggcaca aagggctggc
    52021 tgccctctgt actcccgcac cggcgaagct ggattccacc gcagaagcgg tgtttgccag
    52081 tttcaccggc tccgtatccg cacatttgcg cccataagcg gggaatggat acggaatacg
    52141 cctgcgggga ttgccggtca aaacagcggg tgcagacccg gcattttttc cgtaaacgcc
    52201 ccggtttcag gcgatttttt cttccgcccc taccacctga aagccatgct cttttgcgta
    52261 ggcgctggcg gcggctttcc gttcctcgct gtacggcggc accagccgga tcgacagccg
    52321 ggacttatcc agcacatagg tcacgctgcc ctccggcgtg ctgcgctcca accggcacag
    52381 cagcgggtac ttccggctga aatcagccag cctgcgcttc aagctggcgt tgaaggtgta
    52441 gacgctggca aggttttccg cctcgttcca gttgataatg gtttctttct catacttaga
    52501 cagcttcctc atacagatcc tcctcataat cagtgtaaga tttcagcaca cgcaggctgc
    52561 gcttgaggcg gcggtactcg tccatctcca tccgcagatg gtggtaaaaa tcctcgtacc
    52621 agttttcgcc cacctccgtc tccaccttcc gggccagccc cagcatccgg cgtttggcct
    52681 ccgggtcaac cgtcagtgcc gtcagccatt tcagccgtgt caccgtattg tggtggctgg
    52741 gacagccgta agcgtagaga atcttctttt ctttcatact cagtgtcata atgatttcct
    52801 ccgtttcttt gctgcggagc catgcgcccc gcatgattga taaacttgtt tctgtctgcc
    52861 ggtctgcctg caagccgctc ctgcccggaa tttctcccgg cgtatcggcc tctttggtgc
    52921 gctcgatttc tgtcctggcg tcacttcccc ggaggccata ccccttgcag ccggtcattc
    52981 gattttcaag gttctgtgcc tgtctgcaag aaccagttta ccgaaaaaag aggccctttc
    53041 ccgtatatcc aaaacgtcgg aaaaacgctt gaaatccgca tatttccacg cttatggaat
    53101 cgtgctataa tgggtgtaat cactgaaaaa cgataacgag agagggggct ttggatgtac
    53161 gcaaagctga caatcccgga gcggctcaaa gacctgcggg tggtagataa gcacctgacg
    53221 ctggaacaac tggcggagca gaccggcctg tctaaatccg cgctgggaaa atacgaaacc
    53281 gatgactaca aggacatcag cccgtttgcg attgcaacgc tggcggactt ttacggcgtg
    53341 tccaccgact atctgatggg gctgacggaa aataaaaatc acccaaacat agaacttcaa
    53401 tctctgcatc tgagtgatga tatggtggaa cttttgagca gcggcaaaat caacaaccgg
    53461 cttctctgcg aactggctac ccacccgaat ttccggcggc tgatgataga tatggaaatc
    53521 tgcatcgacc agatcgccaa tatgcgggtg gagcagatga atctggtgtt ggaggccacc
    53581 cggcagaccg tcatgaacaa gtatgcaccc ggagaagatg acctctatat gcggacgctg
    53641 gaactggggc aggtgcagga aagcgatttt tacagccacg tcatgcacga tgatctggac
    53701 agcatcgtcc gggacatccg ggaagcacac cgcaaagaca agaccaccgc cgacccgcag
    53761 cccacgctgg atgatgtgaa ggaaaaattt gagcaggcca tagggcaggg cagcgataca
    53821 gaaatgctga tccacgagtt ctgcgataag ttgcagatac cctttgagaa gatttcctcg
    53881 gaggacttct cggcgttcct gcggatactg agcctgtcca agcagctcaa aagctccaac
    53941 aatatgaggg gaaaggccag ccccattcct cccaaccgca aggccagacg taagcggaag
    54001 taaaatttcc atatccggaa gcggcaaaaa agccatccgc cgattgattg gcaaatggct
    54061 ttcgtgaagc tgtattctgt tgtagtggtt gtgcccagtt tgggaacagt cactattgaa
    54121 gatgctattc tttgtctttc tgcgatacct gtgcatgggc ttcctgctga acaagcagcc
    54181 ccgcagaaaa gccatatttg aagtggcttt cgttttcctc acactgggcg attgccacct
    54241 gctcccgcag ttcttcaacc agtgcatagt cctctttgct cagacgctcc gataaagtgt
    54301 ccatcagggc ggcgctggct ttgactgcct gcttgaacct cggtgaatca gcgaccaccg
    54361 tttcacaggg ataaaatctg ccgttataca tttcttccag aatcatgcac ccacctccct
    54421 cagacaaaaa tcatctcaga caggatgatt tcctctaccc ggctgcggat gctgttcatg
    54481 cccccgaccc atgcaagctg gtcacgggct ttcagttcct cggtgacgcc ctccgcagcc
    54541 tgcatctgcg agatgataca cgccagtcgg tcatcggcct gctcgttcag atcggcaaga
    54601 tacgtccaga gttttcccga cagaagcagt tcattgtact ggccgggacg gtgttcttgc
    54661 agaaacgcct tgtgtatccg accccaccgc ccgatggggc ggatttcctc cgacaacttt
    54721 agatcgggga tatagtagtc tcccaccagc gtatagctga tgccgttgtg atggattttc
    54781 tttggtaaat gctccatgtt cgacctcctg tattcagttt tttcagaatc cagttgatcg
    54841 tgtccctgtt catgtgaaat tcaaggcaac tgaactcttc acgaacaagt atggcatatc
    54901 cttaccagtc ttttgatagt ttaaaccaaa gctcttttga tttgaccttg tttccgacgt
    54961 gttatacagg tcgatagttt cttttcccag tgtttcagac aattctttta gcgtagtttt
    55021 ctccttgcca ccaagaaaca aggtactatc acagttacct acgattgtat cagcgttatc
    55081 cttgtagatt gcctttagct gagattgtgc ttgaagtatg atacttgctg aaatctctct
    55141 tgaacggatt gttgcaatta acttctcaaa cttaggaatt aagccgatat tagcgaactc
    55201 atcaagtaga cacctcacat ggacaggtaa tcgtccgcca tatacatcat cggctttatc
    55261 acataaaaga ttaaataact gtgagtacat aatggatact acaaagttaa atgtatcatc
    55321 ggtatcggag ataataacaa atagtgctgt ttttctatct cccagtgtat caagctcaag
    55381 ttcatcgtca ctcatcaagt ccctaagttc ctgaatatca aagggagcaa gtcttgcacc
    55441 acaagaaata agaatcgatt tggcagtttt tcccgcagcc aatttgtact ttttatattg
    55501 cttcactgca aagtgtgtag gttctttctt ctccagtgct tcaaagagcc tatctatcgg
    55561 attcatatag gtttcattat cttctctgac ttctgaagca tcaatcatat caagcagagt
    55621 tgcaaagttc ttttcttctc ttggagcttc atagaagata tagccaataa gtgctgtata
    55681 gtagagtttc tcagctttca cccaaaaatc ttcacctgat ttttctccct ctcccttggt
    55741 gtttgcgata attgtctgta ctaatttgag tatgtccttt tcacttctaa gataagcaaa
    55801 gggattgtat ttcatagact ttttgaagtt aatggtattt aggattttta tctcatatcc
    55861 gttatcttca agcatcttac cgcactcaag gactatagtt cctttaggat cggtcacaca
    55921 atatgacgag tgcatctgca taaggttcgg ttttacataa aatctcgttt ttccacttcc
    55981 tgatccaccg attacaagta cattcttatt tcttgcatac tttggatttg ccggtctgcc
    56041 attcatggtt aatcgttctg tttgcgtaag taggatattg ttttgaaact tttcatccat
    56101 atatggctct atatcttttt tcgttcccca tcttgctgag ccatactctt tcccctgtct
    56161 aaacttttta gcatttttcc ctttggtata tacgataatt ttgattaaaa cagctactac
    56221 cactcccatt aaaatatccg taggatgaat gcttggaaga aagctcatgg tgttaagttc
    56281 cagtatacct tgaaatattt tgtctattac atcgccacca gtatatgctc ttacatgatg
    56341 agaaaagata ttgccaacat agaaaaatgc aagataggga atgttctgct ttagaaactt
    56401 tgccttatct tgcaccttaa ataagccttt gatgtctttt agtatcttat ctatcatagg
    56461 ctttgctcct tttgtttatt tttgattttc tcttttgaaa tagagttttt agccatctct
    56521 ttgaacttct caatattctt gtgaatagat tctttccttt ctgctttctt ttctgatttt
    56581 gaaagagcgt gcttaaaagc tttatccatc actttcatat ctttggcttg aaagaataca
    56641 gagtttttac ctgtctcttt atttttcatc actgaaaatt taacaccgta tctattcaac
    56701 tcttttttaa gttctttaag ctcagcttct tccactggga tttcttcaag ctgtcccttt
    56761 ttaaccatat cttttagctt tacttcattt ccgttggcat tgaccagttt ttcaagtcct
    56821 ccgaatttct ccgcttcttt catcaatttt tttaataagt ttaatagaac ttttccagta
    56881 actttaccag catttatctc catatttaag gtctttcttg ctatttcttc attgatcaca
    56941 tcaatatcct cctctcatat catagcttac aagagccgaa taataggcat ctattccact
    57001 tggtgcatta taagcggcgg tcaggacaaa agctcttata tttctaatcc tatattcatt
    57061 ttctcttaat actcctacta agtactctaa atgtcctgaa ttaagtttcc taaatctttc
    57121 ctttactact tgtacagata tctttgtttg gttgatagta atgaaagatt catcatctaa
    57181 taccattaca tctgccagta tctttaatat ttcatcaaac tcttcagcca cactcttatt
    57241 cccaatactc atatgttcat aatatcctgt ttggtgtcgc aattcttcta aacaattttg
    57301 atatatattt ttatagctta tatctatcct ccctcccttc atattctctg aaaataattg
    57361 attcttttct ttgatggaag gatggatgga aatatatgct gaattatttg tagtattatt
    57421 tgtagtatta tttgttgtat tctctggtaa tggttggtgc aaattatcct tttgcattag
    57481 tgcattttga acctgtgcat tagtgcaatc tgcattattg cattggctga ttttgccctt
    57541 atccattggt gcattttgcc ctaatgcatt gggcatagaa ttttttaatg cttctgtttg
    57601 tattttttgt tttttcaaat ccatatccat atataattct tcaagtttat cattattaat
    57661 tgtataccat tttgttttat ctcttggatc cttattgtaa tttccaacta aaagataacc
    57721 tgctttttct aactttgaaa aagttctttt tactgtatca aagctcatat agtcaaagtc
    57781 attttcttgc catgctcgaa ttgaattata tgtccagtat ttaccatcat gataattctt
    57841 tcctgatttt ttatttattt ctatccaata attaatctgc tgtagtatga gtgcttcgtt
    57901 taatcctatt tctctagcaa gagttttatt cgcaactata ggttgttcat caaataaata
    57961 cataactcat caaacactcc tttctttttt tcaaacaaaa agacgatagt tatttctatc
    58021 gtctaaaatc atatataaat atcattttct aaatagagcg tattgcttct ttcactcccg
    58081 ttaaaacggc tataacagct gttccaacca ttggtattcc gtatgggctt aacaaaaatc
    58141 ctaatatcaa tgcttctatc cctatggtaa cctctttttg taaaaaagat gctattgctc
    58201 caaatatgca aaacatcatc aacaggtaaa gtattgctgt tcctatgcca agtaaaaatg
    58261 aaagaaatgc aatgagaata cttaacgcta tactaattgg aaataagata atttttaata
    58321 tccatctcat atatactctc catttatact aaatcatttt aaaatgtctt aaagcgtcca
    58381 ttatagcttc ttctttttca ggcgtacact gtgggatagc ctgattgtct tttttagatt
    58441 tattataatg ctctctcagt tcaattccac atttcttctt aatctgtgca atataaagtg
    58501 tagaaacctt tagcccaaat ttttctaata catactcctt aatttgagca tatgttgctt
    58561 tgctctctgc acttgtcaaa tcaagctcat ccagcttaat ttcaacatct atatgcttat
    58621 cgacatcaag tttggacaat aacgctaccg tctccacatg tgtagaatga gggaacatat
    58681 ctacaggttg tgcctcaaca catttaaatc cgtgaacatt tagatagtct aaatcccttg
    58741 ctaaagtaga aggattacaa gaaacataga caattctttc cggattcatt gaaacaatag
    58801 tctctaatac cttttcgtca catccctttc taggtgggtc tacaactact acattggcag
    58861 tcttaccctc tctatacatc ttaggtacaa tttcctctgc ctttccaaca taaaattcag
    58921 cattatgtat attgtttaat ttagcattta tcttagcatc ttttatggca tcatctataa
    58981 tttctattcc ataaaccttt ttagcctttt gtgccaaaaa taaagatatt gtacctatcc
    59041 cacagtaaat atcaaaaaca gtatcagtat catttaaatt tgcatattca agagccttat
    59101 catacaatac tttagtttgg acagggttta cttggaaaaa tgataatggt gaaatctcaa
    59161 acactaagtc acctatataa tcgtttattt ttccatctcc atatataact atattttctc
    59221 ttcctagaat agcattcgta tctttttgat ttacatttac aaccaaagtc ttaaatccag
    59281 gtatattttc cttaagcact gaagctaatt ctttaagata tggaagtttg tttccatttg
    59341 ctactaatac aaccattact tctttagttg taaaacctat ttttgttact aaatgcctaa
    59401 tcaaccccgt gtgagtcttt tcatcatata tacttacctt ataggccctt atatatgttt
    59461 ttattatttt aacaatttta tcatttatct catgttgtat tacgcatttt tctgtcgata
    59521 ttacatcatg agtcttcttt ttatagaagc caataattgg aataccatta ttcttttgta
    59581 ttggaaattg tgctttattt ctatatctat atggattatc cattcctata gtgtcatgaa
    59641 cttctacatc ctctaactta cctattctcg aaagtacttg tttaacttca tttgttttca
    59701 tttcaagttg tttattataa tctaaatctt gtatctgaca ccctccacaa tccctaagat
    59761 tgtcactaca cactcttttc accctgaatg gagacttttc tattatctca acaatatctc
    59821 cctcagcata atttttcttt actttattaa tcctaacttt aactttatca tgtattaatc
    59881 cgccctctac aaaaactgta ataccattgt actttcctac tcctactccg ccttgaccaa
    59941 tgtctactat atctacaata tacatctcgt ttttcgaaag cataaatcac cctccaatca
    60001 atctattata tttaaatctt tacaaacttt tttctttaaa tttatattaa aatattgcta
    60061 ttaattaaac tatatttttt actaagctac aatattttat tttaccaaaa aaaaaggagc
    60121 ttataaagcc ccttagtaaa taaatttatt atttttctta taaaactcta gttgaacctc
    60181 tatataccac gcctcttgat gcatccacag ttacaacttc tccatctttt actagatttg
    60241 ttatatcagt tgcagacaca ataactggta tatctaaatt tatacctaca atagctgtat
    60301 gtgatgtcat tccaccattc tcagttacta cagctcctgc tttttctata taattgttca
    60361 tttcagcatc agtagcagta gaaacaagta tgtcgccttc attaaagtct acacaagcat
    60421 ttccattctt tattatacga actttacctt ctaccgtttg agtacctaca cctatacctt
    60481 gcacgatttc ttcgcttatt acatgaacct ttattaaatt agtagttcca cttactccta
    60541 ctggtactcc agcagttata actactaatt ccccattatc tatataatca gcttgtctag
    60601 ctgcctcaat tgccttttct ataacttcat ctgtatttcc tgcaacagaa gacttaactg
    60661 gatatactcc ccatgtaaga gctaatttat tcatagtctt ttcattattt gtagtagcta
    60721 ttattggaga ttttggtcta aatttagata ccattctagc agtatatcct gaagaagtag
    60781 atgttattat agctgatgca tttaaatcta ctgctgttgt acaagttgca tggcttattg
    60841 catctgttac agtaacattg ttagtaccat tttcctttag taatctatca taatctaaag
    60901 tctcttctgt tctttttgct attgtagcca tcatttttac tgcctctaca ggatattttc
    60961 cagcagcagt ttctccagat aacattatcg catctgttcc atcatatata gcattggcaa
    61021 cgtctgtaac ttctgctctt gttggtcttg gatttcttat catagaatca agcatttgag
    61081 tggcagtaac aactggcttt gctaattcat tacatttttt tatcatcatt ttttgaacta
    61141 ttggcatctc ttcagttggt atctcaacac ctaagtcacc tctagccacc attataccat
    61201 cagaaacttt taatatttca tctaggttct ctacaccttc ttggttttct attttagata
    61261 ttatttgtat atcagttgca ttattgttct ctaatacttc tcttattgct agtacatcag
    61321 atgcctttct tacaaaagaa gcagctatat aatctattcc ttgacttatt ccaaactcta
    61381 tatcacttat atctttagga gttattgctg gtaaatttat cttaacacca ggtacattta
    61441 ctcctttatg atttttaact attcctgaat tttcaacaac acaaactata tcttctccat
    61501 ttatttcttt tactcttaag ccaactaaac catcatctat tagtatagaa tctcctgatt
    61561 taacgtcttc agcaagacct ttatagctta ctgtacacat ttctttagtc cccataatat
    61621 ctttcatagt tatagtgaac ttttgccctt cttctaaaaa tacctctggg tcttcaaagt
    61681 tacctgttct aatttctgga cctttagtat ctaataatat agcaactggc ttatttaatt
    61741 tttctctaac tttctttgcc atatcaattc tttctttatg ttcttcatgc gaaccgtgag
    61801 aaaagttaaa tctacacaca tttagaccat tttgcataag ttgagttaaa atttcctcac
    61861 tttgtgatgc tggacctagt gtacaaacaa tttttgtctt ctttacatta tttaacatat
    61921 ttgctcctcc tgagatatta ttccacagat ttatttttaa tactaaaact tatatagata
    61981 atatttttgc catctcataa gcttctttat caaatacttt cttttgagct aaagcttcac
    62041 ttatttctaa atcaattatt ttgttttctt ttattcctac aactctagct gattttccat
    62101 ctaataataa ttctactgct cttactccaa gtctacttgc tagtattcta tcagatacag
    62161 ttgggcttcc tcctctttgg acatgcccca atacagtgac tcttaaatct gctcctgact
    62221 cttttttaag ttccttttct aaatcactag cacttccgac tccttctgct aaaacgatta
    62281 tactatgtct cttacctctt ttttgagtag tctttaatct tagagcaact tcatctactg
    62341 taatttctac ttctggtact attatagttt ccgctcctcc tgcaagacct gcatataaag
    62401 ctaaatctcc acagtgtctt cccataactt ctactatgtt aactctttca tgagatgatg
    62461 atgtatctct tatcttacct atagcatcta taatagtatt cattgcagta tcaaatccta
    62521 tagtataatc tgtatatgct aaatcgttat ctatagttcc tggtatgcct atagctggga
    62581 atcctaagtc actaagtttt tgggctcctg caaatgaacc atctccacca attacaacta
    62641 agcaatcaat tttatacttt ttaagaattt ttactgcttt taatctacct tcttctgttt
    62701 taaattcttc acatctggaa gattttaata tagttcctcc cctgtgtata atatctccaa
    62761 cagaagataa gttcatttca gttaaatctt cttctagtaa tcccttatat cctctattga
    62821 ttccatatac tttgcatcca taatatattg ctgatcttac aaccgctcta atcgcagcat
    62881 tcattcctgg agcatctcct ccacttgtta gtaatcctat agttttcatt ttttacctcc
    62941 ttggatttta tgtcaatttt ttattataat cattgttatt gtattctaat ggactttttt
    63001 tgtccctata tcacttttta atcccatcaa gactaaaaaa attaaatgtc cttaaatgtc
    63061 cattttgtga tattatatca caaaacggac aatatttttc atattattat aatatattta
    63121 gatttttata taaaaaatca agcttttact tttatgttat ctttaggaag taactcttct
    63181 aagttattta taaattcatc acatatacaa gtcaatgccc cggtcatttt atatgcttta
    63241 cgtttaccat ttatattttc atccataggg tacaagtata tactgtcatt acctggatat
    63301 tgtttggcta tttctacaat attattagtt gtatcagtat cagacatact atctattttc
    63361 aaatacaatt ttttaccact ttctggctta atattttcta catttctagt tcttcctgaa
    63421 ttgtagtttc tatctgcaaa gtcagtatca tcatttatat cttttatctc tctagctata
    63481 agttttgcat tttcgccttc ttttatactt aatgttcctt ttacatatat tatattatct
    63541 tcattcaaaa tgacattata cttttgtaat aattgaggaa aaactattat ttctatagca
    63601 ccatacaaat cttctaattc aataaatgcc ataatttcat ttcgttttgt tgttttaata
    63661 gttttagtta caatcattcc acccattata acatctgttt cattcatttg taggtatgac
    63721 tcttcttcct cttttaatga atttaatttt ccattatcca tagatgtttt ttcttgtagt
    63781 tctttttcaa attgtgataa aggatgtcca cttacataca ttcccaagac ttctttttca
    63841 aggttaagtc tctctctttc ttcatactct tgccttttag gtaaactgta tatttgttga
    63901 aaatcactat cttcctcact atctccaaaa gcatcaaata aagaaatttg accttgaaca
    63961 tttttctttc tatccataga tacactttct aataaacttt cataaccagc catcagacta
    64021 gctctatttt cacttatatt atcaaaagct ccacatttta ttagactttc tattactctc
    64081 ttatttgtat ctttttggtc taatcttttg actaaatcta ctaaatctac aaataatcca
    64141 ttagattctc tttcagcgat tatattgttt attatattta caccaacatt ttttacagct
    64201 gctagaccaa atcttatatt gtcgccttca actgaaaact ttgaaaaact tttgtttata
    64261 tctggaggta atacatctat ttctagtgct ttacattctc ttatatactc aactacctta
    64321 tcagtatttc ccattacact ggttatcaat gctgccataa attcaactgg ataatatgtt
    64381 tttagataag ctgtctcata tgctaaaacc ccatatgcag ctgcatgtga tttattaaat
    64441 gcatatctag cgaagtctat catatcatca aatattttat tagctatttc ctcttctaca
    64501 ccatttctga tacaccctgc aatttctata ttaccatctt catcaaattt accatgaata
    64561 aaatattgtc tttcttcttc catcacgtcc atctttttct tactcatggc tcttctaact
    64621 aggtcacttc gtccgtaact ataaccacct aaatctctaa cgacctgcat aacctgctct
    64681 tgatatacca aacaaccata tgtaacttcc attataggct ttaatttttc atgtagatat
    64741 gttacatctt ttgggttgct tttatttttt atatatgcag gtatagaatc cattggacct
    64801 ggtctaaata gggatattcc cgctacaata tcttcaaaat tgtctggttt tagctgtttc
    64861 ataaaacttc tcatgccagc actctccaat tgaaacaccc ctagtgtatt tcctgatgaa
    64921 agcatttcat atactttcgg gtcgtcataa tccatctttg aaaagtctat tttttctgtg
    64981 tagtttttta taactctatt tttttctatt aaatcaagag tatctcttat aactgtaaga
    65041 gttcttagcc caagaaaatc catctttaaa agcccaagtt cttcaagtgt tgtcatagta
    65101 aactgtgttg tgattgcatc ttgatgtttg tacaaaggta catattcatc tacgggattt
    65161 tttgatataa caactcctgc tgcatgagta gaagcatgtc ttagcattcc ctctatctgt
    65221 tttgatatat ttattatttc ttttgtttcc gggtcttggt catacaattc tttaagatta
    65281 ggatttgtat ctagggcctt atctattgtc attcctaaag caaatggtat ttccttggct
    65341 accttgtcaa ctttattgta gcctatatcc aatacccttc caacatctct tattgcagct
    65401 tttgcaccca ttgttccaaa agttattatc tgggctacat ggtcatcacc atattttctc
    65461 tttacataat ctataacttc ttctcttctc tcataacaaa aatctatatc tatatctggc
    65521 atggatatac gttctggatt taaaaaacgc tcaaataata gagagtactt tattgggtct
    65581 atatcagtta tcttaagtgt atatgctact atcgaaccag ccgcactacc tctacctggt
    65641 cctactataa tattattttc tttagaaaaa tttataaaat cccaaacaat taaaaaatac
    65701 tctacataac ccattttttc aattacatct aattcataat ttaatctctc tagtatctca
    65761 ttgctaggat tatcatatct ctcttttaat ccattaaaac acaactccct taaataagtt
    65821 tctggtgtat atccttctgg aacatcatac ttaggaagat gtatagtatt aaaatcaaac
    65881 tccacattac acatatcagc tattttcaca gtattgtcaa tagcctctaa tgcgtatgga
    65941 aataattctt ccatttcttc tcttgatttt aagtaaaact catcactacc aaatctcatt
    66001 ctatttgggt catttaatgt ttttcccatt tgaatacaca taagaacatc atgtattttc
    66061 gaatcttctt tatttacata gtgaacatca ttagtagcaa ctagtggtat accagtctct
    66121 tttgataatt ttactaaacc agagtttact tctttttgct caggtaaatt gtgatcttga
    66181 atctccaaaa agaaattttc ttctccaaat atgtctctgt agtcaagagc aaattgtttt
    66241 gctttttcat aatttctatt cattaaagca cgtgctacat ctcctgcaag gcatgcagaa
    66301 agtgctataa tccctttgtt atgtttttta agttcttcca tgtcaactct aggcttatag
    66361 taaaaacctt ctacgtatga agttgaaact atttttatta aatttttata tccttccata
    66421 ttcttagcta acagaactaa atgtccttga tatttgtcat agtttgggtc tttatctctc
    66481 aatccacgtg cagctgtata gacttcgcac cctataattg gctttattcc ttcttttata
    66541 gctgttttgt agaaatctat aacaccaaac atacaaccat ggtcagttat agctattgag
    66601 ctcatgttta gttccttagc cctttttata agttttttca ccctcgaaaa cccatcaagt
    66661 aaactatact cagtatgtac atgaagatga gcaaaatctt tgttcattaa atcacctcct
    66721 gctttataat tttaatttta ccacaaatat atgtcctaaa acaaaaacag agacttttat
    66781 ctcaaaatag gaattaactt caacatatac cattatatgt tataaattaa tgcttattct
    66841 gagattgtcc cctcaattta ttgctatgat ttttttttca aatctgttgc aatttcttct
    66901 atttttctaa gtctatgatt gactccagat tttcctattg gtggttctaa catctctcca
    66961 agttctttga gagtcatatc ctcatactcg attctaatct tagctatctc ttgtaaattt
    67021 tctggcaaac tgctaattcc tatagtctct tgtatgagct taatattttc tacttgtctt
    67081 actgctgcat ttactgtttt agaaaggtta gctgtctcac aatttactat cctgtttaca
    67141 ttatttctca tttcttttac tattttagta ttttgaagac ttaaaagagc gtggtgtcct
    67201 cctataatac tcaacaaatc tgatatttgt tcactctctt tgatataaac aacgtgatta
    67261 ttttttctag ccacaatttt actattaaaa cctaaagagt ttataagttc ttttagagaa
    67321 tctgcaaact cctcattatt agttacaaat tccatatgat agttcttttc tgggtcgctt
    67381 atagaacctc caccaagaaa agctcctctt atgaaagctc tcttacatcc ttcatgctta
    67441 attaagttct ctgaaatttt gtttatagta aaaaagcctt ctttatcttc aagtatacct
    67501 aactttttta ataacatttc tgaacccatt tcgcttgtta ccattaatac ataactgtta
    67561 tttctcttta gcatttgatt cttatttact gagattgtag tattgatatt aaaattcttt
    67621 tttaataatt taaatacttt acgggctatt gaatttaact ctgttgttat ctttagattt
    67681 agcttcttat atccaacgat ttgtatactt ccccctgatt taactagagc tgataattca
    67741 gctatattac aacattcatt cccagatata actcttgcta gttcattttt ggtttcagtt
    67801 gaaaaagaca tatctatcac ctattaacta atttttcttt aacctttttt ttatttcttg
    67861 taaggcaacc tttattatct ccttttcatc atcataccta gctaggtcta ccactctatc
    67921 tagatggata aatttagcat caataaagcc ttcctctttt tgtggaatag tatccatatt
    67981 ctttgcttgc atcaaatacc aaaaaactgt tttatgaact gctctatttt catcccagtt
    68041 ttctttgaat gtatagtgta tttcacctaa atatttcaat atatctgctt ttactccagt
    68101 ttcttcactt acttcacgta atgctgcctc ttggttattt tccccttctt ctacttttcc
    68161 cttgggtaac acccaatctc cattgaactt ccttagtaaa agaatcgtat tgccaaacag
    68221 gactacacca ccagcactaa cctcttctct catcgatgtt cactccttaa aactaacata
    68281 atgataagga tttctacaca attttttaaa atcctttttt actaactttt gttgtaatta
    68341 tgatttaggg ctaactcaat aactatgtta gatatatact ttgcatcatg tcttatataa
    68401 ttatttttta tttcaattaa attcttctct acggttttta tacccatctc ttttaattta
    68461 tctttttgct ttttatctaa caagacttgc tctgctccat cttgtttata taagtcaagc
    68521 atttcttctg gcaatatctc attatttgcg attacataat ctattaaatt ctcatcagtg
    68581 tgctttacta tagcattaac atgctctaat acattgtacc cttctgtctc acctggttga
    68641 gtcattatat ttgctatata tacttttgga gctacagacg atttaatagc gtctacaatc
    68701 ccctctacaa gtaaattggg tattatactc gtatataagc ttccagggcc cataattata
    68761 atgtcagcat cgtaaataga tgcaataaca tcctttaatg gtttgacatc ttttggattt
    68821 aaaaaaattt tatcaataga gcttttttga atttttgact cttcaggtat actagattct
    68881 ccatttatta tattgccatt ttctaatttg gcaactaaat ttacatcttc caaagttact
    68941 ggtaaaactc ttcctgttat agcaaaaatt tcacttaatt tatatacagc tttttcaaag
    69001 tttccataca acccattcat agcagccaaa aacaaatttc caaaactctg accttttaaa
    69061 agcccttctg taaacctata ttgcataact tcattcattg ttggctctat atttgcaagt
    69121 gctaatagac agtttcttat gtctcctggt gggagcattc ctaagtcttc tctcaataca
    69181 cctgaacctc caccatcatc agcaacagtt acaattgcag ttatattttg cgtagtgtgt
    69241 ttaagacctc ttaaaaatac tgattgacca gtacctccac ctatcacaac cacatttggt
    69301 tctctatttt caacaataga cctttgaaat agttttactt tttcattttt tgctttttta
    69361 tatataaata acgatacaat taatgcaatc caacctaaaa ttcctacaat aataagtaat
    69421 ttagccatgt ttgcctccat tatcgcaaca tagagtctct atgtttcttt acaactctat
    69481 atcccttatt agataaatcc tccgctataa gatttgttat agtaactgac ctatgtcttc
    69541 cacctgtgca tccaacacca attactagat gttgttttcc ttcatctata tattgaggta
    69601 ctaaaaaatg aatcatatct aaaagtttca cataaaattc ttcactgatt ttagaattca
    69661 taacataatc tctaacttct ttatcatcac cagttttagc tcttaactct tcaacataat
    69721 agggatttgg aagaaatctt acatcaaata ctaaatctgc atcagcaagt ataccatgtt
    69781 taaatccaaa agatactaca gaaatagtta ggtttggatt atcttcacca gaagaatata
    69841 ttttctttat ttcttcctta aggtctttag gtttcatgtt agttgtatca attatacatg
    69901 tagataactc ctttaaaggt tccattatct ttctttccat ttttatacct tctggaatct
    69961 gcatatcttt tgccaatgga tgatttcttc ttgtcatttt atatctcttt aacaatacat
    70021 catcattaca gtccaaatat accatttcat attcataatt ttctttttct aaataattta
    70081 agctttcatg aagcgcttca aaaaactttc tccctcttat atctatccct agagcaactt
    70141 tatcaatact tgaatttggt tgataacata attctgcaaa tttagttatc aaagcaggag
    70201 gtagattatc tacacaataa aatcccatat cctctaaagc tctcattgtc tcactttttc
    70261 cagatcctga aagacctgta actataacaa atttcattgc taactcctcc cttttatctt
    70321 atgtttatta tattataatg ttttctacta acgatatatc cagattagag tcagatatag
    70381 tcttcatagc tagtttaaaa tcaccaactc tttctggaca atgtgcatct gagcctatta
    70441 taaatttatt ttctagccct tttattttat ttaattgatt tacattcaaa aaagaatggc
    70501 tactatttat ttcaagtcta gtatttgtct tttttgctgt ctttgctact tcttctatat
    70561 acacatcgcc tttatctcct ggatgagtaa ttataaaaat atcattatta cttatcgcat
    70621 ttattacagc ttttgtattg tattcttttg ctttttcagt tttaaaaata tagttattac
    70681 aatgatttaa taagcttctt aatgatgttg gagtagaacc aaaatgatat ccagccataa
    70741 tataatcaaa atctctaata aatttatcat ccatatctat attgccttta tcatctaata
    70801 tgttacactc catacctaaa agtatattta tatcattgta cttatcattt aaataatcta
    70861 cttcttctct cattttgaga atatcttttt tctttacacc atatgcaata tgtttatagc
    70921 tatggtcaga tattcctatt gtttttattc ctttagaaat ggctaccttt acattatctt
    70981 caatagtccc tttgccatga ctatataatg tatgtgtatg ataatcagcc agtattttca
    71041 tttgttaatc ctctccaagt attttgactt cttcttccaa cataacccca aacttggcat
    71101 atacagcact ttttataaca tatattaaat ccaaaatatc tttagcactt gcttctcctt
    71161 gattgactac aaatccacaa tgcttctcag atacttgtgc tcctcttaaa gtaagacctc
    71221 tcaatccagt ttcttctatt aatttacctg caaaatatcc tgttggtctc ttaaatgtac
    71281 ttcctgcact cgcaaaattt aatggctgtt tagttattct tcttgtcgct aaatcatcca
    71341 ttaaactttt tatttcatca taatttcctt cttgtaattc tagttctgct gataaagcta
    71401 tatatccgtt tttcgatatt atactttttc tatacccaaa ttccatctgt tcatttgata
    71461 attcaaatat attgccttcc atatccatca gtcttactga cttcactatg tccttcattt
    71521 caccaccata tgctccagca ttcatggcta gtgctcctcc caatgtacca ggtattcctg
    71581 atgcaaactc aaaccctttt aattcttgct tttggagagc tttacctaaa actgataata
    71641 aagctcctga ctgagcagtc atcctagtat catttatctc ataagaattg aaattatcag
    71701 ctaattctat taccactcct cttattccac catctttaat taaaatgttt gaaccatttc
    71761 caattattaa atacggaata gattcttctt ttaccaactt aagtacattt tttaattgtt
    71821 cttcagttct aggtctaact aatatatctg ctggtcctcc taccctaaat gaaatatgtt
    71881 ttttcatagg ttcatcgact tttatatcct ctttacttaa tatatttaat aaatttatat
    71941 atatatcttg attattcatt gtatttcctc ccaaattata ctgaacttta gctgtcatta
    72001 ttatttaaat tacattagta tatagtatta actatattca ataatatatg attttgctta
    72061 tcatatggat tttatatttt aagcttctta attacaaact atattcaata aatatataat
    72121 aaagtaaaaa aaaaaaaata acaacatata aaagaatata aataatttaa aaaaagcatc
    72181 aatatcactg atatcaatgc tttaaaaggc aatttaattc gtattattta tctaactatt
    72241 tttgacatag caaagataat aaatccctcc aactactatg gaacctccaa cgatatttcc
    72301 aagcgttaca aataccaaat tagttataaa ttgaaccata gttatatttg ctcctaatgc
    72361 cattcccatt ggtataaaaa acatgtttgc aacactatgt tggaatccgc ataatacaaa
    72421 taacattatt actgtccaac atgaagccaa ttttgacatt atatcttgag atgctgtagc
    72481 catccataca gctaaaacga ctaaaatatt acaaagtatt gcttttaaaa acattgatgc
    72541 tatagttgaa ttaaccttag cttctgccac agctatagct tttgtagctg catctcctgt
    72601 caatgtagaa gaataaaaca caagtgcaac tagcagaatt gaacctacta aatttgcaaa
    72661 ataaaccagt ccccagtttc ttaacatttt tcctaatgtt atttttttgt ccataagtgc
    72721 aatagccatt aaggtgtttc cagtgaataa ttctgctcct gccaccacta caagcataag
    72781 acctactgga aagaccatag cacttaaaaa gctagctacc cctgggtcta tcttgccaaa
    72841 agtttgacta actatcatac tccccaatgc cccaaaacct ataaatgctc ctccaaataa
    72901 acctaataga attgcatttt gtgtcgaaat gtttgctttt ttaatgcctg cacttatagt
    72961 tgcttgtgca gtttccccag gtgtcaagaa tcctttactc ataaatcctt cccccttaaa
    73021 aattatactt tcccttgtct ttttatggca ataattatat aataacaata tgatatataa
    73081 taaattttat taacataaaa aatatactat atacttatat atacttgtct catataatta
    73141 tactatttat tatataacaa ctttgttaat ttcgaaataa tttatattga atttgaaaaa
    73201 tttacctttt gattatttat tttacttttt aattattgag gtattatata taatagatat
    73261 atgtttgtgt tattatatat aattacttat tttaataaat atttgaagga gggaatttta
    73321 tgtttgatat atcatttttc gaaatgtcaa tatatgaaat agcagttact acaatatata
    73381 taattaattt catggttata ctaaacctta tctttagaga aaaaagaaac ataaatacaa
    73441 ctttaacatg gcttcttata ttagtattga ttcctgctct tggattcata ctttatatag
    73501 cttttggcag aaatatttca aaaaacaata tgtttagatt gaaagaaaag gatgataaga
    73561 taatcaaaag taatatacta gatactcagg ttaaactcca atctacatct gaaattgatt
    73621 cagatataca tcaacataag gatatgattt atgcattggc taattcaaat aatgctcatt
    73681 ataccaataa taacgatgtg tggatttatg ctgaatctag tcaatttttt aacagtcttc
    73741 ttgaagaact taaaaaagct aaaaaatata taaacataca attttacatt ttcaaagatg
    73801 acaaaatcgg aactgaaata atagacattc ttatagacaa agctaaagaa ggtgttgaag
    73861 taagactttt atttgatgca gttggtggta gaacactaaa aaatagtaca ctttccagac
    73921 ttaaagaaag tggtgttaaa gttggaagct tcttcccatc attcttgaaa attgtaaact
    73981 ttaacttaaa ctatagaaat caccgaaaaa ttgttgtcat tgatggtaag gtaggatttg
    74041 taggtggata taatgttggt gatgaatatt taggtagaaa tccaaaattt ggtctatgga
    74101 gagatacaca tactaagctt actggtgatt gcgtaattga cctaaatatg agattcatcc
    74161 ttgattggag atatacaact aaagaagatt tagatttaga aaagtacttt atagaagaag
    74221 atacaaattc taatcatcct tctgaaaata ttgggattca gatagtatca agtggtccag
    74281 atatatcaga acttgatgag attaaatatg gttaccttaa aatgattcag aaagctagaa
    74341 agtatatata catacagagt ccttatttaa tacttgactc aacttttata gatactttaa
    74401 aaatagcttg tttatctggt gttgatgtga gagttatgat tccttctaaa cctgaccatc
    74461 cctttgtata ctgggcatca tattcatatg ctggagaact tttaaagttt ggagctaaaa
    74521 tctacactta tggtcaaaat gcatttttac atgctaagac aatagttata gatgactcta
    74581 tatgctctat aggaacagct aatatggata tcagaagttt tgagcttaat ttcgaagtta
    74641 atgcatttat gtactcttca aaaaaagcat tggaacaaag aattatattt gaaaatgata
    74701 ttttaaattc taaggaaata acattagata tatataattc taggtctact tatataaaaa
    74761 tcaaggaatc aatttcaaga ctattatcag cagttttata aatcttttat tttataaaaa
    74821 ttttacattt tggtttattt tttaaagttt tttatatgta ttattttttt tttattgaat
    74881 ttagctcatt ttttttattt tttttaacta ttctgaatta ttttatatta taatagacta
    74941 gtatagtttt tatttttaat aggtaacttt tttgcattgt ttttataaat aaagaaattg
    75001 ttgtatatta ttctttcttt attgttctgg ttttgttcac ccacataatg ttggggtcaa
    75061 ctcatggtta attattttat gaaaaataat aactttaaaa agaaaagggg gatttgttat
    75121 gtgcgatttt atttcacaca acaagcaatt atttgacgaa ctagatatat ttattgactc
    75181 tcttacaaca aaggaaggtg cgttaataca agttctacat gaagcccaag gtatatttgg
    75241 atatcttcca aaagaagttc aacttcatgt tgctagaaaa cttggagtag ctcctgcaaa
    75301 ggtatatggg gttgttacat tctattctta ttttactaca gaacctgtag gtaagtataa
    75361 aatcagtgtc tgtctaggaa ctgtatgttt tgtaaaaggt gctgacaaaa tcttaagtgc
    75421 ttttgaaaaa cagcttggca taaaagttgg agaaactact agtgatttta aattttcttt
    75481 agagggttta agatgtttgg gggcatgtgg tttagctcca gttgttactg taaatggtaa
    75541 agtttatggt aaagtgaaac ctgaccaagt aagcgaaata ttagatactt atagagaatt
    75601 agaattgaat tgttaagagg ggtgaataaa taatgcgtaa agtaaattca tttgatgaat
    75661 taaaagtttt agctgatgag ctgaaaccta atcttagctt aagaaaaaat tattctaagg
    75721 aaaatgtatt aagaagagaa ttacttgttt gttgtgatac tggatgtacc tcttcaaaca
    75781 gtctagaaat agttagtgaa ttagaaaatg aaataaagaa atcaggaatc caagataaag
    75841 ttagtgttcg tttgacaggt tgttttggtt tctgtgctca aggtcctatt gtaaaagttt
    75901 atcctgataa cgttttctac gtaaaagttg agccatctga tgctgaaaag atagttcaaa
    75961 gccacttaat tagaaataca gttgtcgagg aattattata tgaggaagaa tctttaagta
    76021 aaaaagttga agaacaagaa gaaatgtctt tctacaaaaa acagttacgt atagctttaa
    76081 aaaattgtgg tcttatagac ccagatagta tagaagaata tattgccaat gatggttatt
    76141 tagctctagg caaatgttta acttctctta ctcctcaaga agttattgca gaggtaaaaa
    76201 cttctggcct tcgtggtaga ggtggtgcag gtttccctac tggctctaaa tgggaagctg
    76261 cttcaaaaaa tccagctggt ccaacagata aaaagtttgt tgtatgtaat gctgatgaag
    76321 gtgacccagg tgcatttatg gatcgatctg tacttgaagg tgaccctcat agtgtactag
    76381 aagctatggc aatatgtgga tacgctatag gttctgatac aggatacata tacataagag
    76441 cagaatatcc aaaatctata gaaagattaa aagtagccat agctcaagct gaaaaatacg
    76501 gtttacttgg cgaaaatatc ttaggcactg gatttaactt taaattagaa ttaaaatatg
    76561 gtgcaggtgc tttcgtttgt ggtgaaggta cagcgctaat gcattcaatt gaaggtagac
    76621 gtggtgaacc tagaatgaaa acttatagtt cttctaagag tggtctatgg aaatctccta
    76681 cttgcttaaa taacgttgag acttttgcta acatacctgc aattatactt aagggtggag
    76741 attggtttgc caacttaggt actgaagatt ctagtggtac taaagtcttt gctcttggtg
    76801 gaaaagtaga aaatgtagga cttgttgaag ttccaatggg tactacttta agagatattg
    76861 tatttgaaat tggtggggga atccctgaag gtaagaaatt taaggctgtt caaacaggtg
    76921 gaccatctgg tggatgtata ccaactgaac atttagacac tcctatagac tttggttcat
    76981 taagttctat cggttcaatg atgggctctg gtggtatgct tgttcttgat gaatctgact
    77041 gtatggtaga tatagccaag ttcttccttg agtttactgt tgaagaatct tgtggtaaat
    77101 gtacaccttg tcgtataggt actacaagat tattagaaat attaactaaa attacagaag
    77161 gtaatggtac tctacaagat ttagatgacc tagaaaacct tgctgaaact atacaaactg
    77221 cttcattatg tggtcttggt aaagctgctc caaaccctgt tttaagtact cttcaatatt
    77281 tcaaagatga gtatatagct catgttgtag ataagaaatg tcctgctggt aaatgtcaga
    77341 acttactttc ttactttata acagatgact gtaagggttg tacaaaatgt tctagagtgt
    77401 gtcctgctgg ttgtataaca ggttctgtta aggaacaaca tacaatagat acttctaagt
    77461 gtctaaaatg tggtgcttgt atagataatt gtacatttaa cgctataatc aagaaataac
    77521 ttatttggag gaggaataac aatgagttta gttaatttaa ctattaatgg caaacacgtt
    77581 tcagcaccta gtggaacttc tatcttagat gctgcgaaat taataaatat aaaaatacac
    77641 aacctttgtc atttacatat gaacgaaata gataagcttg acacttgtgc ttcttgtcgt
    77701 atttgtatgg ttgagacaga gagaggctta gttccagctt gtggaacagt tataaaggaa
    77761 ggtatgaaag ttcagactaa tagtgctaaa gcattaaatg ctcgtagaac tattgttgaa
    77821 ttattgttat cagaccatcc acaagattgt tttgtctgtg aaaaaaatgg agattgtgaa
    77881 ttacagacta tagctgctga tttaggagta agaaaaataa gatatcaagg ttccaaatct
    77941 tttgcaggta aagatacttc tactaaatct ttagttaaag accattctaa gtgtatatta
    78001 tgtagaagat gtgagactgt atgtaatgac atacaaacag taggagcatt atctggagtt
    78061 aatcgtggat ttaatacttt agttagtaca ttctttaact ctgatatggt tgaaacagag
    78121 tgtacattct gtggtcaatg tatatctgtc tgtccaactg gagcattaac agaagtagat
    78181 aatgtaccta aactttggga tgttttaaat aaaaaagaaa agactatagt agtgcaagtt
    78241 gctcctgctg taagagttgc tattggtgaa gagtttggat tagaaccagg gtcaatctct
    78301 acaggaaaaa tggtagctgc tttaaaagca cttggatttg aacatgtatt tgatacaaac
    78361 tttggtgcag attttactat aatggaagaa gctactgaat ttatagaaag aatccaaaaa
    78421 ggtgaaaatc ttcctatatt aacaagttgt tgtcctgcat gggtaaactt cttagaacac
    78481 aattatccag acaagttaaa cttagcatct agctgtaagt ctcctcaagg tatgtttggt
    78541 tctatagcta aaaactacta tgcacctaaa atattaggaa tcaatcctga agaactttat
    78601 gttgtatctg taatgccttg tgtggctaaa aaatatgaag cttcaagaga agaattaagt
    78661 gaatctggaa tactagatgt tgacctttct ataactacta gagagttagc taaaatgata
    78721 aaagaagctg ctatcgattt acctaatctt caagaccaag attttgataa tcctctagga
    78781 aaatctacag gtgctgcatc tatatttgga gcaagtggtg gagttttaga agctgcccta
    78841 cgtacttcat atgagaagat aactaataag actttagata atgtaaactt cactaatgtt
    78901 agaggtctta aaggaattag ggaagcaagt atagatgttg atggaactac agttaatgtg
    78961 tgtattgtaa atacacttaa aaatgctaga aagattatgg ataaggtcag aagtggagaa
    79021 tgtaaatacc atatcataga agttatggca tgtcctggtg gatgtgttgg aggtgctggt
    79081 caaccttatc accatggtaa tactgaaata gttgatagac gtgcaaatgc gttatatgaa
    79141 atagatagaa ataaggctat tcgtaaatct catgagaatc ctgatttaca agctatttat
    79201 aaagacttct ttggagaacc aaatagtgat gtagcccata aataccttca tactcattac
    79261 tttgataaga gttgtgtcta tggagagtgt ccacaagaat gtgcttgtga agaagctaag
    79321 tagtcataac aaagtataaa atttattctt aaaaaaggat tgtcttaaaa tacttttacg
    79381 taatttaaga caatccttat taatttcaca aattaaatta tatttctaaa tagacttatt
    79441 ctttaattaa cctatctatt aattaaagaa tacctgccat tctcataagt tgttttgcat
    79501 ttgtagtttt acatcttcca tcttttatct tatagtcaaa atgaatttta tcgtctacat
    79561 agtactctga gaaatgatag tttacaactt caacatctcc actacctgaa aggtcacata
    79621 gctcaaagtc atgtgttgtc accatactta taacccaggg ttttgataat ttctttactg
    79681 cctcacttgc tccaataatt ctatcagcag agttagttcc cttaaaaatt tcatctacta
    79741 gaaccaacat tggcagttct tttaaactat attgaatcat tgattttatt ctaagaatct
    79801 cagcatagaa agttgatatt ccttgactta catcatcttg tattctcata gaagtaaata
    79861 tagccataca ggtcgcatca aaactcttag cgcatactgg agcacctgca tatgccagca
    79921 ctaagtttac accgatactt cttaaaaatg ttgtctttcc agacatattt gaacctgtga
    79981 taatacaagt ctgagaattt aattgtatcg aatttgctat gacacttttt tctgctatta
    80041 atgggtggta tacctcttca actttcaatc ttggagtatc ttcatatttt attactggaa
    80101 aacatgtatt ttcctttaca tgagaaataa ctgttaaact tattagagct tcaaattctc
    80161 caatagattc aaaccaatct ctaatttgat ttccatatat acctttccat cgctccaaag
    80221 cttctgcaca atgatagttc cacatcaaaa caccacaggc aattatatat agtatctgat
    80281 tataccttag gtttacagca tttcctattg aatttaattg tttaacacct cttgaaactc
    80341 cacttccctt agaaagttta tcttgtaatt ctttcaaata tgcactctca aacgtctctt
    80401 tttctaaaac ctcaaacatt ttttcatata cttgtatact tctactaaat gaaaataatg
    80461 gagaaagagt ttgcataagt cttggatatc caaaaccact aaaccctaac tggattataa
    80521 aaagtaaaaa aacaggtaaa acaggtaaaa atcctaacat acaaaatata aaagataaga
    80581 ttgttgcagt tggtagaccc caagtaaaaa tatgcatcca ttttggtata tacacattct
    80641 tattttctcc atactctata aacgattcta tactactatc tgaattattt ttttgctctt
    80701 taccaattat attactgagt atttgaatat gaatagaaaa atcatctttt gataaaagtt
    80761 cttttatagc cctctgtctt tctaagatta cattctcatc tggattttca ttccacaaat
    80821 atttggctaa aaaatttttc ccataagatg tattagctac acagatatat tgatacaatg
    80881 atgctcttcc aaataagtct aaatcttttg cttgcgaatt ttcatctttt aagtattctt
    80941 taccatctat tttaaattcc ttccaaccat ctccaaatct cttaatatac ttatttaata
    81001 cttcgctctt acttttaaaa tatgtttctt cttcttttac tttactatga ataactaaga
    81061 gtgctataaa tattattatt gataaaactc caacaaaaat acctactata tttttattgt
    81121 aaaatccaat agcaaaccct gctatcacta tcaaaaaaga tattaatctt gctgtactta
    81181 tgttatttga ctttttactt aagagcaatt ctttttcagt actactttct aaaagtttac
    81241 taaaatattc tattcccata aaaaattcca actttcttgt tatataatga aactcaaata
    81301 taaaattaat ctagtattat tataatctaa cactgtactt taaattatcc ttatataata
    81361 tatcttatta ataaacatta tatatataca acttgatttt aatttataag gtgtaggaca
    81421 taatcctaat caatatttaa ttaaatgatg tgtagttcac tattattcag tactacttag
    81481 tatcgattaa ttttgataat tttttagtaa attcttgagc tgcatcgtat cccattcctc
    81541 tttgtctaaa attcattgct gctacttcta atatcatagc agtatttctt ccaggtttaa
    81601 caggtacaac taatttctct acattaacac caagtatttc ttcgtattct ttatccagtc
    81661 ctagtctatc ataatactta gtttcatccc aagcttctaa atgaactact aaatctatca
    81721 ttttagaatt tttgattgcc cctactccat ataaacttct aacatctatt attcctattc
    81781 ctcttatctc aagaaaatgt cttattagtt caggtgactg acccacaagc atactttcat
    81841 ctaattttct tatttctaca gcatcatctg caactaatct attacccctt tgaatcagtt
    81901 ctaatgctgt ttccgacttt cctatactac tttcaccttt tatcagtaca cctataccat
    81961 aaatatctac tagcaccccg tgaattgttg tgtgtggtgc taacttatta tccaaatagt
    82021 ttgacagtct atttataagt ctagtggtat ttctatttgt gctcaaaagt attcttttat
    82081 attttttagc cttttcaatt acatctggct tgatttccaa gtctctagaa attattaacg
    82141 ctggtatttc atatgagaaa aatctatcta gtacttcttc tcgaatatga ctatccatta
    82201 atccaaaaaa attatattct gtctttccaa ctatttgtat tctttcatat gcaaaataat
    82261 caaaatatcc tgcaaactgt aatcctggtc tatttatatc ttgagagtag acataataat
    82321 ctacatcatc aggcatgtat actactttta acttcaactc ttttattatc tctcttattg
    82381 atactttatt gctagtttcc attaattttc ttcctttcta aaaaaattat aaacattatc
    82441 tgcaatgctt ttattcattc catctacttc taaaagttct tccatagtag ctttttttat
    82501 agcctctaca tctttaaagt gatttaataa tgatttcttt cttttttcac ctattccttg
    82561 tatatcatct aagatagatt ttgtcaaagc tttatttctt agacttctat gatatgttat
    82621 tgcataatta tgaacttctt cttgtatact agcaataaat ctatataaat tagttgtttt
    82681 atctagttct atttcttttt ctttgcatat caatccttta gttctatgct tatcatcttt
    82741 gtacattccc caaagaggta tatctacatc attgagttct agtacttttt ttactgcact
    82801 aacttgtcct tttccaccat ccaacaatat caagtcaggt aaatttccat gttttaatct
    82861 tctatctaca atttcagcca tagaatcata atcattaggt ccaataactg tctttatttt
    82921 atatctccta tattccttct tatctttttt agcattcgta tacactacca tagaaccaat
    82981 cgaatctaca ccttgtatat ttgatatatc ataagcctct attcttattg gcaacttttc
    83041 tagatttaat atttgtttta attcttccaa tgctccaata cttttttcat attttctttt
    83101 attcatatca gaaaattttt caagatattc tacagcattt ttcctaacca tttcaactaa
    83161 gcttttcttc tctccctttt gaggaactct tattgttact ttttgcccct ttttagatga
    83221 cagccattct tctaatatag aactatcttc tatctcatct tctataatta attctttagg
    83281 tatatactct tgttcattat aaaattgttt tacaaatgaa cttaatatag aagctcttgg
    83341 actatccatt acaccttcta atataaaatg ttccctacct acaatcttac catttcttat
    83401 aaaaaatacc tggacacaag cttcattatg agctcttgcc attgcaacca catcttggtt
    83461 taagtcactt acagtagcat ctattttttg tttctgtatc atctcttcaa ggctctttat
    83521 cttatcccta tatacagcag cctcttcaaa tctaaagttc atagaactct cattcatttt
    83581 ttcctttaaa agttccatca atttttcttc tttaccagat aaacacatta taatctcttc
    83641 tatcattttt ccatactctt ctttagaaac atttcctgta caaggtccta aacatttatt
    83701 tatgtgtaga tttaaacaag gtcttgtctt attctttatt gctttatcta tatctatttt
    83761 acagcttctt attggatacg tactacttat aagctccaag gtatcattca cagcagttat
    83821 attggtatag ggtccaaaat actttgcctt atctttaaga actcttctaa cttttaagat
    83881 tcttggataa tcttcattcg tagttacttt tatatatgga taagttttat catctctaag
    83941 cactacatta tactttggtc tgtatctttt tattaaatta cattcaagta ttaatgcctc
    84001 taactctgaa tccgttataa tatactcaaa cttatatata ttcttaacca tagattttac
    84061 tttagatgta tggttttttg aagattgaaa atactgcctt accctatttt ttagagatat
    84121 ggcttttccc acatatatta tatggtcgta tttgtctttc attaaatata ctccgggctc
    84181 agaaggtaat ttttttaaat gctcttgtat atcaaacatc tcgccctcca aaacattaaa
    84241 ttttatacta aaattatacc ctaaaatatg aattttaaaa agttatacaa tttccagtgc
    84301 tttagacaag ttaaagtcca tattcccttc tataattgat ttgaaaacaa ttttaaccat
    84361 gaagagtaat atggacttta ctttgtatta ctatttacaa cttatatttg actaatttac
    84421 ttataacaaa acagtttatc gtatatctgc tctaaaattt ttcacaattt atataactta
    84481 agaaaaatat ttctttaaga attgacctgt ataagaacca tcaactttag atacttcttt
    84541 tggtgttcca gttgcaacta tagttccacc cttatctccg ccttctggtc ctaagtctat
    84601 tacataatca caagtcttta taacatctaa attatgttct atgactataa ttgtatttcc
    84661 tgtatctgct agtctttgta atacttggat tagcttgtct acatctgcca tatgaagtcc
    84721 agtagtaggt tcatctaata tatacaaagt ctttcctgtt ggtcttttac taagttcagc
    84781 agctaatttt atacgttgcg cttctcctcc tgataattga gttgaaggct gtcctaattt
    84841 aatatatgaa agtcctacat ccattaaagt ttctaatttt ctttttatat ttggtatatt
    84901 ttcgaaaaac tcaacagctt cttccacatt catatctaat atatcagata tagttttatc
    84961 tttgtatttt acttgcaatg tttctctatt gtatctttca cctttacaaa tttcacaagg
    85021 aacatataca tctggtaaga aatgcatttc tatttttatt attccatcac ccttacaagc
    85081 ttcacatcta ccacctttta cattaaaact aaatctacct tttttataac ctcttgcttt
    85141 agcttcatta gtagatgcaa aaatatctct tataaaatca aatacacctg tataagtagc
    85201 aggatttgaa cgaggtgttc ttccaatagg actttggtct atatttataa ctttatctat
    85261 actttcaatt ccttttatct ccttatgctt accagctctt tgcttaagtc tattcacttt
    85321 acttgcaaca cctttgtaaa gtatatcgtt tattaatgta ctttttccag aacctgaaac
    85381 acctgttatg catgtaaact tacctaatgg gaactttaca tcaatatttt tcagattatt
    85441 ttctgatgct ttaactattt ctataaaact tccatttcct tctctagtag tctcaggaag
    85501 caatatttgt tttttaccac ttaaatattg acccgttatt gagttctcat tttcaattat
    85561 ttcatctaaa gtaccctgtg caactatttc accaccatgt atacctgcac ctggaccaat
    85621 gtctataaca taatcagctt ctctcattgt gtcttcatca tgctctacaa cgattaaggt
    85681 atttcctaaa tctgttagat gtctaagagt agttataagc ctatcattat ctctttggtg
    85741 aagccctata cttggctcat caagtacata taaaactcca accaatgcag aacctatttg
    85801 agtagctaat cttatacgtt gcgcttctcc tcctgataaa gttccagctt ttcttgataa
    85861 atttaaataa tcaagaccta catctcttaa gaacatagct cttcctttta tctcttttat
    85921 aatctcatga gctataaatc tctgtttttc tgtaagattt atattttcca taaagtcaat
    85981 caattcattt acagataagt ttgtaacttc cattatattt tttccatcaa ctaaaacaga
    86041 taaaacttcc tttttaagtc tcatcccttt acactttgga catggtctct ctgccatgta
    86101 ttcttcaatc ttatctctca tgtaatctga attagtttct ctatatcttc tctctaagtt
    86161 tactattaca ccttcaaagt atgatttata atttctagtt cctccaaatt ttgactcata
    86221 agtaaactct acaattatat tatctttacc ataaagtaat tcctccacaa aatcatctgg
    86281 taaatcttta aacggtgttg tcaaagatac attaaaatgc tctgctaaac tttttatcat
    86341 tttactgtaa taagtgtcat cattaacacc tgttgaaccc catgctgcca ctgcaccttg
    86401 ttttattgat aaatctttgt ttggtactac taattctggg tcaacttctt tactttcccc
    86461 aagaccatta caaacatcac aagcaccaaa gggagcatta aatgaaaaca tccttggaga
    86521 taattcttct atgccaattc catgttctgg gcatgcaaat ttagttgaaa aaagaatttc
    86581 ttctccatct attatttggg ctattacaag accatcagat agcttaactg cagtttcaat
    86641 tgaatctgct agtcttgatt ctattccatc tttaattact attctgtcca ctacaacttc
    86701 tatattatgt ttttgatttt tatttaagat tatatcatca ctaacttcat agttctcacc
    86761 attaactcta actcttacaa atccttcttt tttgatatta tcaaagactt ttttatgtgt
    86821 acctttttgt cctctaacaa ttggtgaaag aatttgtaat ttcgttctat ctggaaaatc
    86881 tagcatttta tcaactattt cttgaatagt catctgactt attttttcac cacatgtagg
    86941 gcaatacaca tctcctactc ttgcaaataa tagtctcaaa taatcatata tttctgttac
    87001 tgttccaacc gtagaacgtg ggttttttga ggtagtcttc tggtcaattg agatagctgg
    87061 tgataaaccc tctatatatt ctacatttgg cttttccatc tgacctaaaa attgtcttgc
    87121 ataagcagat agactttcta catatcttct ctgcccttct gcatatattg tatcaaatgc
    87181 caaagaagac ttcccagacc ctgacaaacc tgtaaaaaca ataaatttat ctcttggtaa
    87241 ttctaaatct atatttttta aattatgttc ttttgctcct cttattataa ttttatcttc
    87301 cataatgttg tttttacctt tctatttttc caatttttct tctaattgtt tcactttatc
    87361 tctaagttct gctgctcttt caaattgtaa gttttgagca gcttccatca tctctgattt
    87421 taatttatct atattagctc ttatttcgtc tttatcatct gtatcagata ttccatatac
    87481 aacttcttcc tctgcaacct tagtagcttc tatactatct cttatatttt tctgtatagt
    87541 tttaggaatt atattgtgct cttcattata taaattttgt atatctcttc ttctctttgt
    87601 ttcatcaata gcattttgca ttgagtcagt tatcctgtct gcatacataa ttactcttcc
    87661 attttcattt ctagctgcac gtccaatagt ctgtattaaa gctgtttcgg aacgtaagaa
    87721 accttctttg tctgcatcta aaattgctat aagtgaaact tctggtatat ccaatccttc
    87781 tctaagtaag tttattccta caaggacatc aaacttccct aatcttaaat ctcttattat
    87841 ttctgttctt tctaatgtaa ctatatctga gtgtaaatat ttaactttta tccctatttc
    87901 tttaagatag ttagtcaagt cctcactcat ttttttagtt aaagtggtta taagcactct
    87961 ttcatttttt tctatagttt tagttatttc tcctaccaaa tcatctattt gattatttat
    88021 aggtcttact tctacaattg ggtctaaaag gccagttggt cttattatct gttctgctat
    88081 tgtctctgag tgttgaattt cataaggtcc tggtgtagca cttacaaaca acatttgatt
    88141 tatatttcct tcaaattctt gaaaatttaa tggtctattg tcaagtgcag aaggtaatct
    88201 aaaaccattc tctataagtg atgtttttct agacctgtct cctgcataca tacctcttac
    88261 ttgtggtata gtgacatgtg cctcatctac aattatcaaa taatcatctg ggaagaaatc
    88321 cattaaagta tatggtcttt ctccttctga ccttcctgta atatgtctag aatagttctc
    88381 tatcccctga caaaatccta tttcctttaa catttctata tcatacttag ttctttgttc
    88441 aattctttgt gcttctaata gcctatcatt ttccttgaaa aatttaattt gttcttgaag
    88501 ctcgttttct atttctacaa ttgccttttc aattctttct ggcgttgtta catagtgaga
    88561 tgctgggaat ataacaacat gatttctagt accaacaatc ttccctgtta cataatcaat
    88621 ttctgttatt ctatctacct catcaccaaa aaattctatt cttattgctt tttcatcatt
    88681 actagctggg aatatttcta gtatatctcc tctaactcta aaggtacctc gtgtaaagtt
    88741 tatgtcattt ctttcgtatt gtatctctat taaccttttt attacatcat ctctgtctct
    88801 ttgcatacct ggtcttatag ataacataag ttctttatag tcttttgggt caccaagtcc
    88861 atatatacaa gatacagatg atattattat agtatctctt ctctctaaaa tagatgctgt
    88921 agcagaatgt cgaagtttat ctatttcatc atttatgctt gcatcttttt ctatataagt
    88981 gtcactgtga gctacatatg cttctggttg ataataatca tagtaactta caaagtattc
    89041 aactgcattg tctgggaaaa attctttaaa ctcactgtaa agttgtgctg ctagagtttt
    89101 attatgagcc attattaggg taggtttctt aacttgttga atgatatttg ccattgtgaa
    89161 tgtttttcct gaacccgtta cacctaataa tgtcgaaaat ttctcatttc tatttataga
    89221 atccactatt gatttaatag cttctggttg gtcacctgta ggtttgaagt ctgattttat
    89281 tttaaaatcc atagtatcac tccttttttc gaacattagt tcctgacatg tattattata
    89341 tcataccttt ataaaattgt ttaagttatt ttaaatattt tacccattta aattttactt
    89401 aaatctaaaa cataaaaaaa gacttctatc tttgcaatag aagtctataa ttcatggaaa
    89461 ataaatactt tattcaactt acataataaa atcaaatgct tctttttcaa ctccatctaa
    89521 ttttaaatca aacattttta atatagttgc tgcatgtgct ctcaggtctc cttcttctat
    89581 aaccacacca cctttaatat ttggtccaaa agctataaat ggaggtctat tacctttagt
    89641 aggtaaatga ccatgtgtcg caacagcaaa cttgtaatct tttatatctg aatcttttat
    89701 aatacttcct tcgaaatcat ttccaaatga aattccttca agaccttcaa tcaccatttc
    89761 aaaatcacct tcaagaccaa ggttagatgc ttcctctttg gtaaaaatat tctctatgaa
    89821 taattcttct ttaaattctt caagcagttc tcttaattct gttaacctat ctaagttatt
    89881 tactattatt tgagctgata ttccagaaga atttacatag gcatcaaaat ccttaacttc
    89941 tccattttca acttttataa atccatttga tttaagcaag acatttggat ttataacttt
    90001 atcaactcta agatgaccat ggtccccaag tattataaaa tttgtatctt tgtatgtacc
    90061 tgcatccttt gtagcctgaa taatatctcc tagccattta tcattcattt ttaatgcttt
    90121 atctacttcg tcattaaata agccataatt atgtcgtgta tggtccaatg tagctaaatg
    90181 tattagcatt aaatctggtt tatattcctt tattatatct actgaacagt ttactccaaa
    90241 taaatccatg ttgggttcta atttccaatc aatataatga caatgtctat tgtaaatacc
    90301 gtccattata accttactgg agcttcgttc aaaagtttct cttgggtcat cttctctact
    90361 aggagcccat atttctgcaa tattataatc tatatttgga tttgctccca ttacaggcca
    90421 taatacacta gaggtagtta gattattttc ttttgctaca tctataatag tttttacttt
    90481 tatgtccttt gaataccagt accagtcttt attttcttta tttgggtcaa atttttcatt
    90541 atgatatata ccatgttttt taggatatac tcctgtaacc atactagtat gacacggata
    90601 tgtcagagtg ggataaacac aatttatatt ttttactatg gagcaatttt ctagtataga
    90661 tttaaagttt ggtagatttt ttacaaattc caagtctttt tcaaataaag aatctactga
    90721 taatactata acctttgtca tttacattac ctcttcattt atatattatt gaatactttt
    90781 aatcaactta caagtctata taattattta tttgttttat ctattactgc ttgcattgca
    90841 gaagttcctt tgcttactac atcttttgga tttgcattta aatcagtaag ccccttttta
    90901 atctcatcca ttagcttatc agttgcttcc ttgtatccat ttaacattgg aagtgcaaca
    90961 gcatattgta attggtcagt tgcaacctta tattgaggtt tttctttata taatgtttgt
    91021 aacttttctg agtttgttgc atcttcagtt gttggtaagt atcctgttga tgaactaaac
    91081 tgaacgattt tatctgtttg tgtcatccat tctataaatt ctgcacttgc ttctttttct
    91141 ttatctgttc caccttcaag cataactaga ttagctccac ctgtaggagt tgcgtatttt
    91201 tgattttttg gtaggaatgc tgttgatatt tcaaacttac cttctgtttg ttgcataagt
    91261 cctgctaaat ctgctgttga tgtaaatatc atacctactt taccatttac aaaatcttgt
    91321 tttgctacgt cccaggcatt ataatcttct ccaggaggca ttttcattat tccagctttc
    91381 atcatatctt gccagaattt aactggtgct tgtcctgcct cattattaaa tgctactttt
    91441 ttaccattag ttatttctcc tccactttgc attaccatag cttcatagaa ccaaatatct
    91501 attggtgttt caaaaccata agtatcttct gatttatttg tcattttact agcgtaatcc
    91561 ttaagctctt cccatgattt tggacctgaa gggtcaagac ctgctttagt tgccatatct
    91621 ctatttatgt ataatattgg tgtacttcta ttaaatggca taccaacaaa cttatcctca
    91681 taatatgaat ttgttaaaag accagaaaag aatctttctt gatatgtttt atcaaaatat
    91741 ttactcatat ctactaaagc acctgctgat gcgaatgtac ctgtttgagt tatttctaat
    91801 aatgttacat ctggttggtc tttagatgtt aaggctgcct gtagttttgt tgcattttca
    91861 tagtaatctt tctgttttaa catatttact tgtacatcct tatgttcact attaaattgg
    91921 tcaaccattt tttgagcagt ttctccatta gttccaccta aaggagccca gaaatctatc
    91981 ttcgtaatat tgctatcagt cttttctcct ttagatgcat ttgcatcatt agaatttgaa
    92041 cacccaacta aagaacctaa taataatgtt attgctaatc ctgttgctaa ttttctttta
    92101 agcttcattt tcatcctcca aatttagtct ttaatttttt attttattcc tgaatacata
    92161 aaagcttctt ttactttttt attcgcaaat atataaacaa gaattattgg taaaattaga
    92221 attatatttc ctgccattat tacattccat gcagtaattc cttctgaaga tttaaggagc
    92281 gcaactccta ttggtaaagt tcttattgca tctgtatttg tcattactaa gggccagaaa
    92341 taattattcc aatggtatat aaaattaaat aatataaatg ttattactgc tggttttgcc
    92401 ataggaacca ttattctaaa cattattttt aagtcagaag cattatctag tttagctgct
    92461 tcaatagttt catctgaaat ctgcataaaa ttctgtctaa gcagaaatat cccaaatgaa
    92521 gatgtcataa atggaattat aagtggaata taagtattta taaatccaaa cttactcatc
    92581 atcatataga ctggtagaaa agttacttgt ggtggaatca taagtcctac aagcaaaagc
    92641 ccaaataaca ttttttcacc tttaaattta atcttagaaa atgcatatgc tgctggaacc
    92701 ataactaaaa attgtaaaat taaaacactt cctgttacaa atacactgtt tagtaaatat
    92761 ctaaaaaatg gacctgaatg ccatgctgta ctataatttt ctaacactat atcatttggt
    92821 ataatagtag gtggtaattg catcatttct tgtggatttt taaaggatgt cgatagcatc
    92881 catataaaag ggaatataaa tattgcagat aaaagtaata gaattaatat ttccattgat
    92941 tttgatgcta ttttttttat atcaactttg tcttttttga taggttgtat aatatccatt
    93001 gtctcctcct tttggataaa tcttataata tctacttttt ctttattgat aatgaacttt
    93061 ctttgataag agtctaaagt tagcatatgt agcaatcata actatagcta acaaaaacat
    93121 actcgctgca ctcgcttgcc ctatgtcata gtatataaat ccagactcat atatataata
    93181 agataacatg ttagttgaac ccactggccc accttgtgtc attatactta tctcatcaaa
    93241 tacttgaaaa gatgcagttg ttgttgttat cataagaaaa aatattgttg gtgataatag
    93301 cggcaatgtc actttaaaga aattcgttat tttgtttgca ttatctaatt tagatgcttc
    93361 atatatatag tttggtatgg atttaagacc tgataatact attaagatat tgtatccaag
    93421 agtcttccat attcctatta gaattagtga cattaaagct gaactttcac ttgaaagcca
    93481 ctttgatgtt ggtaaatgta atgtcgatag tatccagttt aaaaagccat attgtggctc
    93541 cataatccaa agccacaata cagctacaga tactagtgaa attacatgtg gtgtaaagat
    93601 tatactttgc gttaaattgt gtattataga atttttattt agccaaacag caagtactat
    93661 ggctagtgat atagatatag tcaccataaa aaaagtataa attgcagtgt tcgttatagt
    93721 ttgtataaac tctggattct ttaaaagttc tgtataattg tttaagccta cattttcaaa
    93781 ggttgaacta agaagactac cagatttgaa gcttagagat atcgtcttaa ttattggata
    93841 gactataaac aataaagttc ctataatagc aggtgttata tataaatatg ctttattaaa
    93901 ttgctttttc ataaatatct cctttaattt tagaatacat acttttacat acttttacat
    93961 cttctattct ttctccattt ttatcaaaat aatataaatc tttttctaat acacttattc
    94021 ttaaagttct tccaatatca aatatatcat tttccgtttt gactcttatt tctctattaa
    94081 gaacagataa acaatatata gtttcatttc ccaacacttc tttagtcaca acttttccaa
    94141 ataggctaaa tacatcatca ttttttccat ctatgtttat actctctagt gttgcttttt
    94201 ctggtctgaa tccaaattta atatcatttt ttatatctag tatgttcata caaggactcc
    94261 ctataaattg agctacaaat aaattcttag gattttgata tatcttcttt ggagaaccta
    94321 tttgcattat ctttcctttg ttcattataa ctatcttatc tgccattgac attgcctcaa
    94381 cctggtcatg agttacataa acaaatgtag attttagttt ttgatgtaat tgtattaatt
    94441 ctgttctcat ttgatttctt aatttagcat ctaaatttga caatggttca tccattaaaa
    94501 atacctttgg atttttaacc atagctctag caagtgccac tctttgtctt tgacctccag
    94561 ataagttgcc tggtttcaca tttaaatatt ctctcaatcc tactacatca ataacttctg
    94621 ttataagttt ttctcttttt tctttttcta cttttttgtt tttaagacca aattctatgt
    94681 tttctctaac agtcatatgt ggatataatg cataattttg aaataccata gctatatccc
    94741 ttttactagc atccgttttt gtaacgtcat tttcttcaat ttttaacttg cctgaagtta
    94801 tatcttcaag acctgcaatc attcttaatg tagtagattt accacatcca cttggaccta
    94861 ccaatactgt aaaagaacca tcttctatag taagatttaa attttctata actgtatttt
    94921 ttttattata agattttgtt acattttcta gtcttatatc tgccattttt atcctcccaa
    94981 aatatttaat ttaattctac gatttactta tagattaata atactcttaa tttgtaaagg
    95041 atatattaag acaaagtgaa tatatagtaa acttattaaa ataaaggtta aattataaac
    95101 attttttgtt aaatatactg aagttaagat gatatacagc ttatgttagc tataattttc
    95161 tatatttttc atattcatgg tttgctatca caatgtgatt ttaatttatg aagagtcata
    95221 taaaactatt tattttttat ataatttggt ttgaaaataa aaaagacctc tatgggggat
    95281 agaggtcttt aagtggggga gattgagtaa tttatttttg ctatattaag ttttacaatt
    95341 atattatgtc ccaaatttac aaggtttaaa cataaaaaat aaaacttttt taatttttat
    95401 aaatttaccc aaaaaaaatt acgtggctac gtcctactct cccaggcagt ttcccaccaa
    95461 gtaccatcag cgctagagag cttaacttct gtgttcggaa tgggaacagg tgtatcctct
    95521 ctgctattgt aaccacataa tctttatgct taatatttct attaagtttt taataagttt
    95581 atagaaccta gtaatactag tttctttaga gtta
//
LOCUS       DAELXX010000002        81880 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.002, whole genome shotgun sequence.
ACCESSION   DAELXX010000002 DAELXX010000000
VERSION     DAELXX010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 81880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81880
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.002"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(142..816)
                     /locus_tag="KPH29_000083"
     CDS             complement(142..816)
                     /locus_tag="KPH29_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1465728.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVELIDDFEVVLDEFMDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIDILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GIGYII"
     gene            complement(968..2875)
                     /locus_tag="KPH29_000084"
     CDS             complement(968..2875)
                     /locus_tag="KPH29_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG1465729.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQEENKKFTLSVLVAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVVIIKLSGFNIKVSNGLSIEAILYATL
                     IFISIFIIMAVKGFITICRSSVINLINISKKQEKIPKVGIGIYILAIVSLIVIGYGYY
                     LSTNIRTGDITSVLPVIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNIIAINNI
                     NYRLNKNYKTYAIIAIIITTTISTLGASVAMKHIHKNAQEQRNVYTISIVSMDKDEVN
                     KKQIENIIKDTNKIKYSVNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVNGNDKE
                     LKEYGERLVSGNNVIELKSPQTIASIATKEVKTKLDGQLYNISRGEVKVPVLGTGLNQ
                     SIIVVNDDTYFKLRDKGQQVYFYGAKVFQEENSQVMFNNIEKNLSLKNGYIQNGYKAQ
                     GESQWMKFAYVVLVFLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKIGTTEKDINR
                     AILKEVAIFYTLPMLSATISSYFALRLAGDLLMNDLSGIYILSLVICLIIFVIYGAVS
                     VNKFKSVVYKN"
     gene            complement(2876..3628)
                     /locus_tag="KPH29_000085"
     CDS             complement(2876..3628)
                     /locus_tag="KPH29_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1465730.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFIAIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPAVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMIN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            complement(3946..5100)
                     /locus_tag="KPH29_000086"
     CDS             complement(3946..5100)
                     /locus_tag="KPH29_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG1465731.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINILLLPDIEKINKKINVTNKDIIELLKDKKYILLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYSSLNLLKLSA
                     IMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSSLIIPTVAGFLSINIGYNYTLMILSLFGCLAYGLIVLLNYLQE"
     gene            complement(5103..5570)
                     /locus_tag="KPH29_000087"
     CDS             complement(5103..5570)
                     /locus_tag="KPH29_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG1465732.1"
                     /translation="MFFKRKKEASTINSYIDGKIINLGEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            complement(5584..6072)
                     /locus_tag="KPH29_000088"
     CDS             complement(5584..6072)
                     /locus_tag="KPH29_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1465733.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSLF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIKQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            complement(6086..7417)
                     /locus_tag="KPH29_000089"
     CDS             complement(6086..7417)
                     /locus_tag="KPH29_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG1465734.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARILGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMIEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            complement(7431..8996)
                     /locus_tag="KPH29_000090"
     CDS             complement(7431..8996)
                     /locus_tag="KPH29_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG1465735.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFLFTFLIKKFDMKTPGREEDNEETKLYTKNEYLETKDEKGNKLSKASQQASE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKVVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            complement(9165..9950)
                     /locus_tag="KPH29_000091"
     CDS             complement(9165..9950)
                     /locus_tag="KPH29_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG1465736.1"
                     /translation="MRLEEMIFLNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            complement(10117..11316)
                     /locus_tag="KPH29_000092"
     CDS             complement(10117..11316)
                     /locus_tag="KPH29_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HBG1465737.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEELSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILKDVIKG
                     QIGYMYLPSMSSKNGSINNDLKIIGDYINTLEKHKALIIDIRGNLGGSDRYWQGIVSK
                     LIKNDVKINGYRIYRNNNEIVKKYTNARNIKLNPVENLPASVKENAPKEIIKDFNDFE
                     DTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKETKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNCIRKAL
                     ELENINY"
     gene            complement(11838..13175)
                     /gene="xylA"
                     /locus_tag="KPH29_000093"
     CDS             complement(11838..13175)
                     /gene="xylA"
                     /locus_tag="KPH29_000093"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HBG1465738.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNVTDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
     gene            complement(13271..14821)
                     /gene="xylB"
                     /locus_tag="KPH29_000094"
     CDS             complement(13271..14821)
                     /gene="xylB"
                     /locus_tag="KPH29_000094"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBG1465739.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNIIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVMLDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYINTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVASLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSQVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPISENVDTYKIYHRLYKRIYKSLKDDFKLLDE
                     VVNTKINN"
     gene            15029..16189
                     /locus_tag="KPH29_000095"
     CDS             15029..16189
                     /locus_tag="KPH29_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBG1465740.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNQNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            complement(16345..16749)
                     /locus_tag="KPH29_000096"
     CDS             complement(16345..16749)
                     /locus_tag="KPH29_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG1465741.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANDDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNASLLSDDE"
     gene            complement(16770..17252)
                     /locus_tag="KPH29_000097"
     CDS             complement(16770..17252)
                     /locus_tag="KPH29_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG1465742.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            complement(17272..18081)
                     /locus_tag="KPH29_000098"
     CDS             complement(17272..18081)
                     /locus_tag="KPH29_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG1465743.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            complement(18074..18853)
                     /locus_tag="KPH29_000099"
     CDS             complement(18074..18853)
                     /locus_tag="KPH29_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG1465744.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            complement(18853..20931)
                     /locus_tag="KPH29_000100"
     CDS             complement(18853..20931)
                     /locus_tag="KPH29_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBG1465745.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
     gene            complement(21722..22243)
                     /locus_tag="KPH29_000101"
     CDS             complement(21722..22243)
                     /locus_tag="KPH29_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBG1465746.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAINLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFNEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKINDIQMVDGKGIFIEELEKLLKEYNLE
                     IPIVGFDELREDK"
     gene            22409..22975
                     /locus_tag="KPH29_000102"
     CDS             22409..22975
                     /locus_tag="KPH29_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG1465747.1"
                     /translation="MDTKKVTFSSICIVINIVLGMVISMINIPLLFLDTVGTVLGAVV
                     LGPFWGALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSSY"
     gene            complement(23192..25255)
                     /locus_tag="KPH29_000103"
     CDS             complement(23192..25255)
                     /locus_tag="KPH29_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBG1465748.1"
                     /translation="MKAGEIEFLSYLEGSNKSFVVPVYQRNYNWKKEQCKRLFDDLED
                     IIDSGFRTHFLGSVVSIYSMGKEYLIIDGQQRVTTVSILLIAMCQIIKEFGESKGIIT
                     TEEQIKECYLINKHSRDKDGKIKLKPIAEDRKAYYELFEEEMNTDRNSNIVSNYLYFY
                     NRIKEDNISIDDLFDAIQKLTIVEIELKNGEDDPQLIFESLNSTGLDLSEADRVRNFV
                     LMGRSSKLQEEFYNNYWYKIEKNTSSNVSSFIRDYLTIKERSIPNKNKVYFSFKRYVS
                     DNGIDIEILLKDLLKFSNYYREISYSCTDDNDVNKVLKRINKLEIFVSYPFILELFND
                     YNEGIINKKVLVESLNLVECFILRRIVCDVPPNALNKVFMNLGREIKKFSDYKENYSN
                     ILRYIFINKKSSQRFPTDSEFSNAFETKDIYNTKSKNKLYILERLENYNNKEIVDLEN
                     LINNNMLNIEHIMPQTLTNKWKESLGDNYKEIHDKYLHTIGNLTLTGYNSKLSNKTFE
                     EKKEMEDGFKDSRLYLNKYISSIDKWNEEEIKNRTKILLDRAVEIWKYPSVTYEPPSN
                     SENIFTLEDDDVNFTNESIVSFSILGESYKAKNWTEFYEQVALTLYDLDSVRFNSIVD
                     KTYKRDFIDNIKSRDVYKLRKGIKISENIYLETNLNTEAKLNIIRFLLNEFDIELNEV
                     IFSIK"
     gene            complement(25636..28971)
                     /locus_tag="KPH29_000104"
     CDS             complement(25636..28971)
                     /locus_tag="KPH29_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBG1465749.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYANDSDLVLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLHGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEKLRKSLTEKRKVNTVNYDFKIDEVNEFETRKRYINIELKLAGWE
                     FGKDIGEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVYGFYKKSELQLMIDRRKSKKSLSSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLMGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGEEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKAETLTEKLFNIKVSMVKELQDIKYIEGKYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMVTAEKLSKLGTIPQIQQQKYTIEKAMTEDFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEDRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGDTPVSQLVRKLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            complement(29021..29449)
                     /locus_tag="KPH29_000105"
     CDS             complement(29021..29449)
                     /locus_tag="KPH29_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1465750.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDYLLACEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            complement(29439..29819)
                     /locus_tag="KPH29_000106"
     CDS             complement(29439..29819)
                     /locus_tag="KPH29_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBG1465751.1"
                     /translation="MRNIPNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTEALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYESKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            complement(29852..30160)
                     /locus_tag="KPH29_000107"
     CDS             complement(29852..30160)
                     /locus_tag="KPH29_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1465752.1"
                     /translation="MNQIELDLYHDMVNIYKEADLQCNYKPTRFLQLINNKGALLAAK
                     ELINKEGATEGFTRLWECKRLDLSLEVLVLKEKYKELFTDEEREICINRLKDYGYKFN
                     "
     gene            complement(30193..31377)
                     /locus_tag="KPH29_000108"
     CDS             complement(30193..31377)
                     /locus_tag="KPH29_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBG1465753.1"
                     /translation="MVDEAKILDVVSISRENVKKFDGERSYLSTGNLDFNKISNLEIV
                     TYENKPSRANQTVNIGEVIFAKMKDTKKTLVINKTNKNIIVSTGFYVLKPSKEILPQY
                     LYHYLNSSYFLNQKNRLSKGATQSALNNEGLANIKIRMYNLKVQEKVVRVLDKAQELI
                     DKRKEQIEVLDELVKSRFIEMFGTPSKNEKNWEISEIGKYLDVLTDYHSNGSYETLRD
                     NVTLLDTKGYALMVRTTDLENNNFEKGVKYIDEHAYNYLEKSKVFGGEVIINKIGSAG
                     KVYLMPFLNKPVSLAMNQFMLRFNEDKVNHIFLYNLLLTSYMESKIKEKVRGAVTKTI
                     TKDAVRKINIIVPPIRLQNQFANFVKQVNSLKFEMETSLKELEDNFNSLMQKAFKGEL
                     FN"
     gene            complement(31381..32844)
                     /locus_tag="KPH29_000109"
     CDS             complement(31381..32844)
                     /locus_tag="KPH29_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HBG1465754.1"
                     /translation="MITGELRSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDEV
                     ETKNEAEATLLGVSFEKIFDDEHQHLRWSKFSNEGNSEKMYEIVQNEVFPFIKKLHGN
                     KESAYAKYMGDAIFKIPTPLMLSKIVDGISNLELSKDRDTKGDLYEYLLSKVATAGTN
                     GQFRTPRHIIDMIVRLIKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHSSLFLVQGLKE
                     HFNNGMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKDKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRVLKIGGRCACIVPDGVLFGSTGGHK
                     SIRKEIVDNHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYS
                     LDDKRNEIKDSDIEDIIKRFDNLDGEVDRKRTEQSFFVDVEEIRENGYDLSINKYKEV
                     VYEEVVYDAPSVIIGRVKVLEKEIVDGLEELERMLEV"
     gene            complement(33354..34223)
                     /locus_tag="KPH29_000110"
     CDS             complement(33354..34223)
                     /locus_tag="KPH29_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBG1465755.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIREVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKDVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIGTVKC"
     gene            complement(34243..36159)
                     /locus_tag="KPH29_000111"
     CDS             complement(34243..36159)
                     /locus_tag="KPH29_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HBG1465756.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNATKDGHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSGVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGV
                     SKEGFDFESEDGSLAHIIFMISATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVSKLNNEEIKSV"
     gene            complement(36162..37082)
                     /locus_tag="KPH29_000112"
     CDS             complement(36162..37082)
                     /locus_tag="KPH29_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HBG1465757.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLADISYLSISGSLPPGIDDNYYEDIFKICKNKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            complement(37184..37936)
                     /locus_tag="KPH29_000113"
     CDS             complement(37184..37936)
                     /locus_tag="KPH29_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG1465758.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTINKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            complement(38233..38850)
                     /locus_tag="KPH29_000114"
     CDS             complement(38233..38850)
                     /locus_tag="KPH29_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1465759.1"
                     /translation="MNVIKKFIFVILIINILGCGISLILQSSLGSDAITLLNEGIHIK
                     LGISYTIAGLIYNGTLLLIALLLNKKSLGLGSCVYVLVAGIFIDLYIYLLLDFNISSL
                     GIIIRIMSFIIGHILMCSGFAMLIKFDIGMSPLDAILIYIEGKFKYPYKVLKTIADLI
                     FLILGVYLGGNLGFGTIFSILCTGTTISIVSKMISVNKKRYYSSI"
     gene            complement(38938..40395)
                     /locus_tag="KPH29_000115"
     CDS             complement(38938..40395)
                     /locus_tag="KPH29_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBG1465760.1"
                     /translation="MIKLSKFPKDFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKD
                     RDISKHYESDMTVDKIKFAMKDNNGYYPKRNGIDFYHTYKEDLKLLKELGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKDKVKYWIVINQINLMYHESFNSVAFCVDQVDDVEEAKYQ
                     AIHHQFMATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRIQYFFT
                     DVSFRGEYPAYIKRFFKENNINIVMEEGDDKVLKENTMDFLALSYYYSTTVSASKNTM
                     NPIDNVKNQYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKV
                     HDSYRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKERYKKDSFYWYKNVIRTNGEELKEKVDSF"
     gene            complement(40417..40770)
                     /locus_tag="KPH29_000116"
     CDS             complement(40417..40770)
                     /locus_tag="KPH29_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG1465761.1"
                     /translation="MTTEEHVIDEELVEVAMQIILRAGEARTEIKHALNDLERFDYKN
                     ADLKLAKAKEFMTEAHRAQTNIIQGEASGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            complement(40799..42046)
                     /locus_tag="KPH29_000117"
     CDS             complement(40799..42046)
                     /locus_tag="KPH29_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG1465762.1"
                     /translation="MGRLFMNKFIDLLSEKFTPVVNKITKNIWVQSVQSTIMKVLPMV
                     FVGSLVTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMEIMKNKKFESMSVVA
                     GLAGAGLMLMMIRPEITNEGAIFNFNRFGGEGMLVALVAGLFSGLIMSLFGSFSFFGE
                     DSALPDFVSKWFDSMLPIAVIMFIGWGLIYGLNFDMFEIIVRIFKPVSDIAQTLPGFV
                     LLNFLVVFLYSLGISGWVLSPITYAIQLSAIAANAAAVASGGLATNIYTYEVVQTGWI
                     TLGGWGATLPLVLMMCLSRAGRIKAIGRACIVPSIMNINEPVMFGAPVAWNPFLMLPM
                     WIGTVVISITTYLSMSMGFVTIPSKVFNLWYLPFGISTFMVNQDVRGLIVLAINIVLL
                     FMIWYPFFKAYEKEELKLESQEA"
     gene            complement(42054..42371)
                     /locus_tag="KPH29_000118"
     CDS             complement(42054..42371)
                     /locus_tag="KPH29_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG1465763.1"
                     /translation="MKNIKVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLPETDCLLLGPHLDYMKDEIQKKADEYNVPVACISKTAYGRLDGVKALEEALELI
                     LNK"
     gene            42619..44532
                     /locus_tag="KPH29_000119"
     CDS             42619..44532
                     /locus_tag="KPH29_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG1465764.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     STGEFILSSKKGYKVNLNNNCQTKVDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     ELFVSPATIEGDIVKANKFIGSYNLKIKQSKFLLKLIGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYQEYNLTKLKENIIYILKKYNLFINEYAINNILLHLMITIDRIKKNNY
                     IESVEVVNYIDNNNDISISRDISNFLESEYNLTLTSAELYYLVFQLTNKTTVLNYHEM
                     DTKSLPNYIDEHFVKLTKKIIKNVYDLYFIDLSDDEFVVKFTLHVKNLISRAKNNQVL
                     RNQIPQKLKDSYPLIYDISVYICNQIQILENIEIDEDEISYISLHVGSFFDRQKLLED
                     KILCALITPNYYDLQFKIVRDLEKRFNESIEIIQIFSDTHNLDFDNKVDMVITTLPIS
                     DRCPIPFVYVNPYLNRKDYDNIQSKFIQIKDRKNILTVQNHLEMYFSESLFMKNIYLD
                     SAKDYIKFMGNILYKNKYVKPNYIDDVLIREKMSSTAFNNNVAIPHSMKMDALKTGVC
                     LIVNDKPVKWGEEKVQIIAMIPINEKEKEKFNYIFESFIEILSEWNNVKELTKADNYS
                     SFMNRIAYLIQNI"
     gene            complement(44655..45809)
                     /locus_tag="KPH29_000120"
     CDS             complement(44655..45809)
                     /locus_tag="KPH29_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBG1465765.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKETIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDENNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEELDNGLRNFSKVVKNYLSKDVANVEGAGAAGGLGFAFLAFLNSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYGVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(46091..48706)
                     /gene="mngB"
                     /locus_tag="KPH29_000121"
     CDS             complement(46091..48706)
                     /gene="mngB"
                     /locus_tag="KPH29_000121"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBG1465766.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPERKFFLSRYENIFKELEKNKDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHQEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLVAFNLMP
                     YNRNEIVNAQVITKMKAFEIFDKDNNKLDFEVVHKEVIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEVEHMLEKAYEVCDMVDNDFYTIQVNENGTLKISDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANSTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRADSFIDFDIVINVPNDKAIINVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEEWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGKKFDTIAITLFRSVGFLGKEEMARRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKIPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKEEALVVRFYNSKEETKSANVVFNKNIKTAKWTNLNEKELESISIDNNSV
                     NVECLMNQVKTILVK"
     gene            complement(48850..50775)
                     /gene="mngA"
                     /locus_tag="KPH29_000122"
     CDS             complement(48850..50775)
                     /gene="mngA"
                     /locus_tag="KPH29_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HBG1465767.1"
                     /translation="MEFKKITNENLVFLKQKIKTKEDAIKFLARKLYDEGKLTSYEEY
                     LEAVMYRETLSPTGFERGLAIPHGKSKSVKEAAFAAMTLETPIENWESVDSNNKVELI
                     FLIAVPESEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIVLAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKKADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQEL
                     YNQEGSWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVIGKPVAA
                     LNNGLINWLNGMTGINAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIHVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIIS
                     AILLIITRKQKNQRKIH"
     gene            complement(50905..51630)
                     /locus_tag="KPH29_000123"
     CDS             complement(50905..51630)
                     /locus_tag="KPH29_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG1465768.1"
                     /translation="MEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKF
                     FTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNEDKEPKNGFESDIKKTLELIDYEKIN
                     KVADIIQKSRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVLHDIGKLGEQD
                     MFFALSLSGETKEVIEAAKTAVNKGCKVVALTHICENQLQQIADISLYCYSTIKQVNG
                     YNISDKVPIMMIMRAIIEQYWLR"
     gene            complement(51893..52672)
                     /locus_tag="KPH29_000124"
     CDS             complement(51893..52672)
                     /locus_tag="KPH29_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HBG1465769.1"
                     /translation="MHKMKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPF
                     SRQAIEMVKNDDVCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGK
                     EVFNYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPY
                     YLDSCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSS
                     LIQERVYEHAMLCDKEVKMWLKRNNIELVDFKDIFKSNNYI"
     gene            complement(52665..54092)
                     /locus_tag="KPH29_000125"
     CDS             complement(52665..54092)
                     /locus_tag="KPH29_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG1465770.1"
                     /translation="MMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVPAISNISN
                     IVFSNVDYLFLLGAVSAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNAGVFGGII
                     VGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIYPQAAMNQ
                     FGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVIRGEIPRF
                     MAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALTSFMATVT
                     EPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGARLGSFNGASIIDYVLSFSYGDKAW
                     LVIPVGVVFFVLYYVTFKYIIIKDNVQTPGHEVEIEIGDKVSEKEKNLKLSHGNFSYM
                     AKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLSNTSVQII
                     IGTDVVKVKDEMEMQLDELRKQADA"
     gene            54483..55205
                     /locus_tag="KPH29_000126"
     CDS             54483..55205
                     /locus_tag="KPH29_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG1465771.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECIGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            55402..57069
                     /locus_tag="KPH29_000127"
     CDS             55402..57069
                     /locus_tag="KPH29_000127"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBG1465772.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYSLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMSDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNLENEKVREECYKVLEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENKMEIKPYESIMIYYK"
     gene            complement(57165..58484)
                     /locus_tag="KPH29_000128"
     CDS             complement(57165..58484)
                     /locus_tag="KPH29_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG1465773.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQAAEPLIKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRKTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYADKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
     gene            complement(58525..59271)
                     /locus_tag="KPH29_000129"
     CDS             complement(58525..59271)
                     /locus_tag="KPH29_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBG1465774.1"
                     /translation="MKKLVKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CKAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            complement(59515..61524)
                     /locus_tag="KPH29_000130"
     CDS             complement(59515..61524)
                     /locus_tag="KPH29_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG1465775.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEEIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGESSLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLRDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQSFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEVMLKHFMKRKRAISQGVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNSINE
                     LGYSITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNILNSI"
     gene            complement(61962..63395)
                     /locus_tag="KPH29_000131"
     CDS             complement(61962..63395)
                     /locus_tag="KPH29_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBG1465776.1"
                     /translation="MVLRKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSREVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDIPQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMTKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(63482..65398)
                     /locus_tag="KPH29_000132"
     CDS             complement(63482..65398)
                     /locus_tag="KPH29_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG1465777.1"
                     /translation="MRRYEQLSKEIIENVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASIGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVIIIVLLAAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGVLTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGAFVPSFAQTSVVAAMYFKLKDKKIKELCIPAVISGICGVIEPAIYGITL
                     PRKTPFIYSCVGGAVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDSIVEISKDNNGQGVVEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTIMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKHFKSFVNQGDKIKKGQTLITFDIESISKEGYCLETPVVVTNYSDYVDIIENTQ
                     KHVNNSQELITVLA"
     gene            complement(65472..66332)
                     /locus_tag="KPH29_000133"
     CDS             complement(65472..66332)
                     /locus_tag="KPH29_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG1465778.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKCIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(66683..67870)
                     /locus_tag="KPH29_000134"
     CDS             complement(66683..67870)
                     /locus_tag="KPH29_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG1465779.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGDNKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIESGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(67884..69275)
                     /locus_tag="KPH29_000135"
     CDS             complement(67884..69275)
                     /locus_tag="KPH29_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBG1465780.1"
                     /translation="MSSLKKKKFTMPQTLTIIFSLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIVPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQESMVNSDTNIKEFTKREAMVIAIFTIGMAIMIYGVLRL
                     EWYITEIAMIFTAIGIISGIASGLKQDEIVNSFISGAGDLITAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMVVLTIL
                     VMVFLTIGLYLGF"
     gene            complement(69870..71570)
                     /locus_tag="KPH29_000136"
     CDS             complement(69870..71570)
                     /locus_tag="KPH29_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG1465781.1"
                     /translation="MHYIAWHFNKNGGNNTMKDKLQVLSGAMMIPVILLVVAGVFIGL
                     GSAFANMENVSALGLSGLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKK
                     EKGWGALGGIVLFIGMHTVISTLLASNGINSDTVTAEAFMASGLGEAEAYSRSSLYGS
                     FLGIFSYDCSIFGAIIAGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLG
                     TAMYFIWPPVGLALSKLGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVG
                     GTMEVGGAIYEGVNNIRLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLAD
                     NANKKKAISILIPAIVTAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFN
                     VSIMGESFRNMFPNLLQPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSE
                     EVKLYSKKEYRERQSVDAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNC
                     ATRLRVELNSIDGFYEDGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD
                     "
     gene            complement(71542..72945)
                     /locus_tag="KPH29_000137"
     CDS             complement(71542..72945)
                     /locus_tag="KPH29_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG1465782.1"
                     /translation="MIVNKELIIDYLKSNQENIIKDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGENGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTDDKKDLVESLAYYTEIEELEEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGIYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSSAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(73209..73700)
                     /locus_tag="KPH29_000138"
     CDS             complement(73209..73700)
                     /locus_tag="KPH29_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG1465783.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPNEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(73726..74538)
                     /locus_tag="KPH29_000139"
     CDS             complement(73726..74538)
                     /locus_tag="KPH29_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG1465784.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEAMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLMGYEYKKVLSKEAIKKIYTIGEKYNLNKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSKEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(74551..75702)
                     /locus_tag="KPH29_000140"
     CDS             complement(74551..75702)
                     /locus_tag="KPH29_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG1465785.1"
                     /translation="MINFYQPSRINFGQGTLSELPRIIGKYGKKCLLVTTPNVEPLDK
                     LYKRVTLNLIEKGIEVVHFDRVEPNPTVELINEGFELAKKESVDVVLAVGGGSSIDTA
                     KVLALTFGLEKIDWDYMFSTYTNPFKIYDKVSEKELPLIAVSTTSGTGSQVTQAAVIS
                     RGSEKNTIFHQNCFSKECIVDPELMLTLPERITASTAFDAFTHAFESYINPNANDFTE
                     IMSEKSMELVINYLPKVMEDKNNIEYRTKLAVADTLGGSSLANAGAAAPHPLSEIIGG
                     VTHIPHGEALAIVFPKFAEKFYKDNIEKFAKVARMLNQELEKVEDDEAAKTLGNEIES
                     FLKRVGLKTKMSEFNVTKEQLEQITSCPVLGFLPFGKKEDLVEILEKSY"
     gene            75972..76613
                     /locus_tag="KPH29_000141"
     CDS             75972..76613
                     /locus_tag="KPH29_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBG1465786.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CTNLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTVDGANTFERDWSKVNK"
     gene            76639..78183
                     /locus_tag="KPH29_000142"
     CDS             76639..78183
                     /locus_tag="KPH29_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG1465787.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AVFELATVNKSYTELKEILFSNSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(78400..79026)
                     /locus_tag="KPH29_000143"
     CDS             complement(78400..79026)
                     /locus_tag="KPH29_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG1465788.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
     gene            complement(79161..79877)
                     /locus_tag="KPH29_000144"
     CDS             complement(79161..79877)
                     /locus_tag="KPH29_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentose-5-phosphate 3-epimerase"
                     /protein_id="HBG1465789.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRADNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDEAATKFGLE"
     gene            80292..81506
                     /locus_tag="KPH29_000145"
     CDS             80292..81506
                     /locus_tag="KPH29_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBG1465790.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYEI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITDEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
BASE COUNT        26796 a        14540 c         8807 g        31737 t
ORIGIN      
        1 gcactaaaaa atctaagtat attaattctg aatatctatt ttttaaaata tctatagcaa
       61 ataaatagat atttattata ctaactacaa ctaaaggtag agcaatatac ttagacctac
      121 cttttaaata ttttactagc attatattat atatcctatc cccttcttag tttttataaa
      181 atcatcaaaa cctagttctt taactttact tctcaatcta ttcatattta cagtaagtgc
      241 attgtctgtc acaaactcat cattatccca tagagtcatc ataagacttt cccttgatac
      301 aaccctattc ttatttttta ataataatgt taaaattttt atttcgtttt tagtcaactc
      361 tatactttta tccttatact tatgttttat aatccctttc cccacatcaa acatcaaatc
      421 ttcacaatac aaaatactat tagaatcact ataattatag actcttctta ttataccatt
      481 tattttggta accaatatgt ctatagaaaa tggtttggta atataatcat ctgctccatt
      541 atttataccc attattaaat ccatatcaga atctcttgat gataaaaata ttaatggtac
      601 atttgaaata gctcttattt tttcgcacca gtaaaatcca ttatataatg gtaaatttac
      661 atctaaaagt actacatctg gttttttatc cataaattca tctaatacta cctcaaaatc
      721 atcaattaat tctactttaa atccccattt ttcaagtgct aacgcaatct cttttgataa
      781 atcaatatcg tcctctacta ataaaattct atacatttgt atattctcca ttatcctaat
      841 atttcactat gccatgaatt attaatatca taacatattt tatataaatt tattacccaa
      901 attatttagt taatcaacaa ggagactctt attacaagaa tctcctttct ctttattaat
      961 atatcaacta atttttatac actacactct taaacttatt aacagatact gctccatata
     1021 ttacgaatat tatcaaacat atcactaaac ttagtatata tataccagat aaatcgttca
     1081 tcaataaatc tcctgctaat ctcaatgcaa aataactaga tatagttgca cttaacattg
     1141 gcaaagtata aaatatagct acttctttaa gtatagctct atttatatct ttttctgtag
     1201 tacctatttt taataaaatc ttgtatttct ctttatcttc atatgcatca ctatatattt
     1261 tcatatatat tatacttcca gctacaattg caaagactaa gaataaaaac acaagcacta
     1321 catatgcaaa cttcatccat tgactctcac cttgagcttt atatccattc tgaatatatc
     1381 cattttttaa acttaaattc ttttcaatat tgttgaacat tacttggcta ttttcttctt
     1441 gaaatacctt tgcaccataa aaatatactt gttgaccctt atctcttagt ttaaaatatg
     1501 tatcatcatt taccactata atactttgat taagtcctgt ccctaatact ggaactttta
     1561 cttctcccct tgaaatatta tataactgac catcaagttt tgtcttgact tcttttgtag
     1621 ctattgatgc tatcgtttga ggacttttta gctctattac attatttcca ctaactaatc
     1681 tttcaccata ctcttttaat tctttatcat tgccattcac tttaagagta ttacaaaaat
     1741 cactatattt cattatagta tctgttgtat cataagcttc ttttatatct gttttcttta
     1801 agattgttag ttctggattt acggagtact ttatcttatt agtatctttt attatgttct
     1861 ctatttgttt tttattcact tcatctttat ccattgaaac tatgctaata gtgtacacat
     1921 ttctttgttc ttgggcattc ttatgtatat gcttcatagc aactgaagca ccaagagttg
     1981 atattgttgt agttataatt atagctatta tagcataagt tttatagttt ttatttaatc
     2041 ggtagtttat attgtttatc gcaattatat tatttccatt aaataatatt tttttatttt
     2101 tgattataat atcaaatata acaggcataa ctgatttgaa taagccataa gtacctacta
     2161 ttattatacc tataacaggt agtacactag ttatatctcc agttcttata ttcgtagaaa
     2221 gatagtatcc atatcctatt actataagtg atactatagc taatatatat atacctattc
     2281 ctacctttgg tattttttct tgttttttag atatattgat tagatttatt acactacttc
     2341 tacatattgt tataaaacct tttacagcca ttattataaa tatagaaata aagattaaag
     2401 tagcatatag tatagcttct atgctaagtc cattagatac ctttatatta aatccagata
     2461 attttattat cactacttga aaaaatctag atgttattat ccctataatt attcctgtta
     2521 tacaagaact tattcctata agcatatttt ctacaaaata tatttttcct atagtatata
     2581 aatctaatcc cataaaggac aatattccaa tttctttaga cctattctta aaaaatatat
     2641 ttgttgcata ccatataaag aaaaataaaa atactgatag tatcattatt acagctacta
     2701 acactgatag tgtaaatttt ttattttcct cttgtaacac atttatagca tctccatatc
     2761 ttaataattc aaaattagtc agcacaacta ctgaaaaaac catagatata aaaaatgcta
     2821 gataagttct tatgttactc ttaaaattat tataagcaat tccaagtata ctcacttaca
     2881 tctcacctcc catagaggct agcatatcca taatttttcc ataaaattct tttctactat
     2941 ttcctttgtt taatttacac tttatatttc catcatttat catatatact tcatttgaat
     3001 aacttgccgt tagagggtca tgtgtaatca ttataatagt agcgttgttt tctctgttta
     3061 ttttttctaa tgattctaaa agttcatatg ctgatttaga gtccaaagca cctgttggtt
     3121 catcagcaaa tatgactgct ggatttgtaa ttaatgacct agctgctgcg cctctttgtt
     3181 tttgtcctcc tgataactga tatggatatt tatctaaatg attttctaat ccaaattttt
     3241 tagctataga aaatacctta gcttctatct ccttgctttt cttttttcct aatgctagag
     3301 gaagtgctat attgtccatt agtttcatat tgtctaatag attatagtct tgaaatataa
     3361 accctatttt ttctcttctt aattttgata gttcattatt ttttactttt gatatattaa
     3421 ttccatctag tattatttct cctgaagttt gtttgtcaag tgtagacatt aaatttagca
     3481 atgttgtctt tcctgcacca gatggaccca ttattgctat aaaatcacct tcattgactg
     3541 taagagatat attttttaac acatttgttt taaatccttt tattccataa tcttttgata
     3601 tattttttat ttttaatatt tctttcataa taatagcctc cagttgctaa ttttttaata
     3661 tatatttatt ttaaaggttt tatataaata aatccttaag taaatattac attttaactg
     3721 ttagcattac acttttgtaa catatggaag actattataa ttatagtgga aatatcttaa
     3781 aataaaagca tgattcactt ttttgtataa acaaagaaaa gaagccccta agaagcctct
     3841 aatctataca ataattcttc tctataaaaa accaaaaata caaccttgat tgtggcaaat
     3901 acaggtacta taaacaagtg atattactac tatatattaa aacaattatt cttgaagata
     3961 attcaataag actatcaagc cataagctaa gcatccaaaa agacttaata tcattaatgt
     4021 ataattatac ccaatattaa tactcaaaaa accagcaaca gttggaataa tcagagatga
     4081 acctcccata aatatactta aagctattaa ttggctacta cttctaagtt ttttaggaga
     4141 aacttcaaat attaatcttt ttgaagtaat taaaattaaa ggagtagaaa acacttgaaa
     4201 aacacacagt ataataatga tatttgaatc atttgttaaa gcaaataata gaaactgaac
     4261 tgttatcata atagcactta attttaataa gtttaaactg ctatattttt ttaataaata
     4321 agaaccagcc agatatgttg gtatctctaa taatgactgt aaagaccact ttaaagatat
     4381 ctgagaattt gtagcattta aagctatcat tttatctatt acagtacagt tatttgctac
     4441 caccattgag tataataaga ataaaataca tactagtaag atatattttt tatcttttaa
     4501 taattcaata atatctttat tagttacatt tattttctta ttaatctttt caatatcagg
     4561 taataatagt atatttataa taagtaatac tacaattaaa ccaatcgcta cagataatcc
     4621 aaaatatgag aatctaagaa tcaagtaaga tgctaccata cttccaatag cccatccaat
     4681 cgacccaaaa gtcttaataa atgacaaata attattaata ttatctccac aacctattat
     4741 ccatgtatca tataaactac agcaagtatt taataatcct cctgataaag caacaagtaa
     4801 taagtatatt ataaaagttg cagattcagt agaaaacata ataactgatg caattccata
     4861 aactgataaa ctaattaaaa caattttttt cattgtttga tacttatcag ctaatactcc
     4921 aaataataat tgaaataata tattggttat tgcatagctc gataatataa ttcctctttc
     4981 aaacgctgag taacctaatt ttgataagaa aggtgtgaat tttgttgaag caaaggcaaa
     5041 aataatatat gagaaaaaat agaatatgat aaatttaaat ttaatcgctt tattaatcat
     5101 aatcaatcaa aattgataac tttaatacca ctatttcctt cctgatttaa gtttgaaaga
     5161 tgatatttta ccgtattagg attctctaca ataactaaag gaatatataa agtattttca
     5221 agtaacttat tatcaatttc tactaacttt tcccccatat taacttcttg accttgttta
     5281 acttctaatt taaagttttc tttttttgtt gttgcactat ccaatccaat atgaataaga
     5341 atttgaacat cattttgcaa tttaattcct atagcatggt tagtttgagc aataaaactt
     5401 attactcctt tagtcggtga tttaagaata ttgtcctttg ctatcatagc aaatccatct
     5461 cccattacct tagttgaaaa tactttatca ggaatttctc caagattaat tatttttcca
     5521 tcaatgtatg aattaattgt acttgcttct ttttttcttt taaagaacat tatagacact
     5581 cctctacata attaacttta ttctaattat cattacaaat aacgatatta agcatactgc
     5641 aattgaggaa aaaagagcct tatagctctt tccccattgt agttctttaa tactgcttat
     5701 gatttgttta attccaacaa atttataaat ataggtaaac atgataacaa tagttattgt
     5761 gtaaatttgg aatatctgcc ctaataagac taaatagaaa gcaatcatgt tttcttttat
     5821 ctctttattc ttttttatat tgtaaataac atatgcactg tatactgcac taaagttaga
     5881 gattaaattt aaacttgcat taacatcatt gtttaaaagt tgtgaaaatg aaatgtttgt
     5941 aaataatgat gtcaattcta aatacactat atataaaaca ggtaatatta gaaaacaaac
     6001 cataaccact gaaagaaata tattttcatc ttttaatact gctgttatat tttttatttt
     6061 atttgttatc atgtttttgt tctccttatt taaaagttgg ccagaaatcc ttattagcca
     6121 caatcatttc atctaagatt tcttttgcta cttttgcact tggaacagtt ttactcatag
     6181 taaacgactg ccataatttt tgataacttt tttctatcca tgcttctaca gctaattttt
     6241 cagaagcaac ttgttgttcc attaaacctt tttggaaagt tggaatttta cctatagaca
     6301 taggttcaac catatctttt ccaacaaggc aaggaatttc aatcattgca tcatctgcaa
     6361 aattttcaat agctccatta ttaggaacaa tcagtaaaaa tctttcctga gtattaaatg
     6421 ctaatgcaca agctaattca acaataaatt gagaatgttc acttgcatca aaccatgtat
     6481 cccttgctgt gccatttttt ataattcttt cacattcttc gaatgtatcc ttttctcttc
     6541 tatccataat ttcatttgct cttgtataat ttggattagc atgttcaaca gtttcatcag
     6601 ccattaaata atattttaaa taagtattag gtaatgtagt cggttctatt tctaataagt
     6661 cctttgctgc tctcatagtt tctaaccaac tagattcttt gtgttgacct tgaggtgtaa
     6721 attcctcata tcctttttta gcacaatatt cctgtaattt aggcattaaa tcatttcctt
     6781 ctttgtcctt aatacttgtc caccacccga aatgattcaa tccataatat ctaacatcaa
     6841 agtctttacg agaatttaaa cctaaaattc tagcaaatat ttcctctaaa catactggca
     6901 tatcacaaat attaataatt cttgaatttg gtcttaatac acgacatgct tctgcaacta
     6961 tagctgctgg atttgaataa ttaagcatcc aagcatttgg agaatatttt tccatataat
     7021 caatcatttc gattattgga cctattgttc ttaatccata ggcaattcca cctgccccac
     7081 aagtttcttg accaacaacc ccatatttta atggaatttt ttcatcttgt tcacgtaaag
     7141 catataaacc aacacgaatt tgtgccatta caaaatcaat atcagtaaat gcttcttctg
     7201 gatttgttgt ataactaaat ttaatttctg gatttttctc tctaacaatt attgcacatg
     7261 cttcagcaac ttttctttgt ctctcatcat tattatcata taatttaatt gattcaatag
     7321 gaaatttagc aacactttca attaacattg ttacaattcc agcagtataa gtactacccc
     7381 caccagcaat tagtatcctc tgtctttttc tttccatctt tccttctcct ttataataaa
     7441 ttttcaaatt cttctcttac acttggtact gaaagcccaa taataatctg aatagctttt
     7501 ccattatgca ctaaaccatg tgcacctact accttaaaat cttcttcttt agaaataatt
     7561 gagtcatcct tcaaggttaa tctcaacctc gtagcacagt ttgtaacatc tacaacattt
     7621 tctgctccac caactaattt tatgtattca gatgcttgtt gagaggcttt actcaattta
     7681 tttccttttt catctttagt ttctaagtat tcatttttag tatatagttt tgtttcttca
     7741 ttatcttcct ctctaccagg agttttcata tcaaacttct taattaagaa agtaaataaa
     7801 aagaaccaga taaatgaaaa tataatccct acaacaactt gaacaacata taccttccag
     7861 tgatacattc caagaggaag ccaatttaat acaacccagt taataattcc tccaccaaaa
     7921 tcaccaacaa ctcctaaggc aaacgaaatc gtttgcaagc atgcatctaa aaatgcatgt
     7981 actaggaata aaagtggtgc tacaaataaa aatgtaaatt ctaacggctc agtaattcca
     8041 gttaatactg cagtaagagt agcaggaatc attaaaccta atactttttt ctttttttct
     8101 ggctttgcag ttacatagaa agcaccaaat acacctaatg aaccaaacac cttcgataat
     8161 ccagataatg aaaatccgat tggatacatt tcctttagag tcttagctga agtttgaaag
     8221 tcctgaatat gagtagccca atatactgat gtaccacctg gaacaacaac actatcataa
     8281 caaattggtg catatacaaa atgatgtaat ccagttggta ttaaaatctt ttgtaagaat
     8341 gcataacacc aaactcctat atttccacta tttataaaaa acgtttgcaa atgtctaata
     8401 acattttgaa tatgaggcca aataaaacaa gttaataatg caactggtat cataacaaag
     8461 aaacaaattg caactacaag tgatgagcct ctaaataatc caataaaatc tggtagttct
     8521 ttatcataaa atctattatg aatataaatg acaatacctg atattaacaa agcaccaacc
     8581 ataccagtat ctaatgtttt aataccagca atgcttacaa gtccacttcc agcacttatt
     8641 tcagctgcga aatcaactcc aaagcttttt ccccaaaaac ttaacataga acctacaaaa
     8701 taattaaaag tcaaatacgt tgctaaagcc tccatacaag ctcttgcttg ttgctttttt
     8761 gcaagtgcta ttggtaaaga aatagcaaat aataatggta attgattaaa taccatccaa
     8821 gctccactag atattacacc ccaaaattgg taccaaatat tttctggtgt agcaagtgaa
     8881 cccataattg actgattttg aaatacaatt gagaatccaa ccattatccc ggcaaaagca
     8941 aataatagtg ttggtgtaaa cattgcccct ccaaaacgct gtattttctt catcatataa
     9001 attactcccc tcctcatatt tttagtttat ctttggcctt agatatctat tattttaaca
     9061 ggttaataaa tatatacaat ctttagggta catttggagc ttattcacag tcaaataagt
     9121 ttttgcacat ttttatgttc aaattctcat aaaaaaaaga atgactacaa tttgcaatca
     9181 atcccttctg taatcattct atgcctttta taaatactat atttaataaa taataactca
     9241 atcaatataa aatataacat tgtcaattta taactagggt gcatttgtaa aaatgagata
     9301 ttagtcgctg aaatataaat tgattcatca cttaaatcag tcaatgtatt atttttaaac
     9361 tctgtaattg aaattatttt agttcctatt aattttaatc ttctagcaat attaagtgta
     9421 ttctcattct caccacttag ggatataata atcataacat catttttact gacaatattg
     9481 taaaaagaat catcaaatgt tcttccctca aaatcatata ttatttcttg acatgataag
     9541 aacattctct ttatctgttt tgcaaccaca ttttgaatat cacctgaacc ataagcgtat
     9601 attgttcttg attcataaag aagtttacac aatgcatcat aatctttatt tttttgatct
     9661 tcaacatact taattattga attacatgca atatcaaatg actgattttc tatgacatga
     9721 accttttctg attcccagct taataatact ttaaactcac tgaaaccttt taaatctaat
     9781 tttttaacaa atcttgtaat agttgcacga gacacattac atttcatagc aagttcattt
     9841 attgacatct tactacaatg ttttttattt gcagttatat atttccatat taataaatct
     9901 gtatcattca atttattata atttaaaaaa atcatttctt ctaatctcat aaactattat
     9961 cccctttaat taaaaatcgt cataaatatt ataccaatat tcaacattaa attacagcgt
    10021 attcttatat ttactattta gcccataaaa ttgactataa aaaatactgc tataattgat
    10081 gtttaaaaaa atgtagtttt aattttattc ttttatctaa taattaatat tttcaagttc
    10141 tagtgctttt cttatgcaat tatcatcttt aaaatcagca gtccttttac aatcttttac
    10201 tttaaattct ggaatagttt taaattcctc atcacatatt ccattcttat ttaaatacat
    10261 acaacttgcc attctaacta ttagaccact attttttaaa ttaaacaata cagggtctat
    10321 acaccctcca tctccgccag tagtttcacc aactaaagtc gcaaatttag tttctttaca
    10381 aaacattgca aatgactctg atgatgaata tatttttcta tcaacaagta agtatatatt
    10441 acccttaaat tttgaatcat ctgtagattt tatagtatag gaagtatctt caaaatcatt
    10501 aaaatctttt ataatttctt taggtgcatt ttcctttaca cttgctggta aattttctac
    10561 aggatttaat tttatatttc ttgcatttgt atattttttt actatttcat tattatttct
    10621 ataaattcta taaccattta ttttgacatc attttttatg agtttagata cgattccctg
    10681 ccaataccta tcacttccac ctaaattacc tcttatatca ataattaaag ctttatgttt
    10741 ttctaaagtg ttaatataat ctccaattat ttttaaatca ttatttatag agccattctt
    10801 tgatgacatt gatggtaaat acatatatcc aatctgaccc ttaataacat cctttaaaat
    10861 taattctttt tttacaaaaa tatcatctgg catttttatt ttaagattca tagagtcata
    10921 tctatccaca acttttttat cattaagaaa atcataccaa tcattattag agtatgattt
    10981 cttaaaaagt tcatatcttt tcttattatc tattacttct gtgtgtttat tatttaaatc
    11041 agataatata gatgacagtt cttctataaa ttcttcatca ttttcagtgt tttttattcg
    11101 ttttgtatat tcatccttat ttgctaacca atcaatatta ttaagtctct tatttacacc
    11161 tagatatgga tatcctgtct ttacaacatt atacacataa ttaaagtcat ccaccttttc
    11221 ctcttcagac aactgattct tttgagaata agtagaatta cctaataaga ttataaatgc
    11281 taaaataatt actaaaaata atactttctt tttcatgtaa ttctcatcca ctttcagatt
    11341 ttatatttta atacattgta tcattatttt attatcacct aaacagttaa aagaaattct
    11401 tgaagatata ttccactatt attctattta aaaattttta tttgtatttt caaaattatt
    11461 tcatgaaagt tataaaataa caattatatt caaatatcta ttttactgct atatcctgaa
    11521 agtaaattct tatcagtgat atactaaaaa agatatgcta aaaaatttat ccattataat
    11581 gacttaaatt tagatacttt actgattttt atttattagt tgagaaaggt attgataatt
    11641 aaaaatattg ataactatag aaatgattat tttttataga aattttaagg atataaacta
    11701 attatactta agtctatatc cttaatttat catgattatg agaattttta caaatactta
    11761 tctttgtccg ttttagaata aaaacaagaa catatcctgt tattttattc aaacatccaa
    11821 gtttaattac tacacaatta cttattagaa attgtatcta cttcatatat atatctgtta
    11881 agtatagctt ctagcatttc ttgtcttcca gacttattct ttataactgg catttgcagt
    11941 gcataagatt ctaactcttt aaatccaacc ttaccttcta ctatatcctt acctatacct
    12001 tcactaaaac ttgcatatct ttctttagta aagttttcaa atacctcatc ttctaataat
    12061 ttatgagcta ttaataatcc ctttgcaaat gtatccattc ctgcaatata agctaggaat
    12121 aagtcttcta cctcaaaaga tgctcttctt actttagagt caaagttaaa tcctcctggt
    12181 gctattccac cttgctttaa cacttcatac atagcaagtg ttgcatcata tatatttgtt
    12241 ggaaattggt ctgtatccca acctaataat aggtctcctt gatttgcatc tatactacct
    12301 aaaacattgt ttattcttgc tatatttaat tcatgttgga aagtatgtcc tgcaagtgtt
    12361 gcatggtttg cttctatatt cattttgaag tatttatcca gattatactt tcttaaaaat
    12421 cctaaaacag tagcagtatc aaaatcgtat tgatgtttag taggctcttt tggcttaggc
    12481 tctattaaga attggccagt gaatcctatt tccttagcat aatctacagc catttgtaag
    12541 aatcttgcaa agttatccat ttcaagttct gtattagtgt taagtaaagt ttcataacct
    12601 tctcttccac cccagaatac aaaattttct cctcctaagt acttagttat ttccatagct
    12661 tttttaacct gagctgctgc atatgcatat acatctgcat tacatgttgt agatgcacca
    12721 tgaacaaatc ttggatttcc aaacatattg gcagttcccc ataataactt tatacctgtc
    12781 ttttccatat taacttttat atactcaact atttcatcta atatcttatt tgtctcttct
    12841 aaatctcttc cttctggagc tatatctctg tcatgaaaac agaaatactc aattcccatc
    12901 ttagacataa attcaaatcc tgcatcaact ttaatctttg ctatctcaat tgggtctgtt
    12961 acattattcc aaggtctctc tactgtgcct actccaaatg ggtcattacc ctctccacat
    13021 aaagtatgcc aatagctcat tgcaaatctt aagtgttctt tcatagtctt attacctacc
    13081 acttgttcag gattgtagta tctaaatgct aattcattgt cagattttac tccttcaaat
    13141 tttatctgtc ctattccttt aaatatttca ctcatttcat gtcctcctaa tattttacac
    13201 atataatata tgtcgtatgt tttcttaatt taaaactatt ttctatcttt atttttcttt
    13261 ttaagctaat ttaattattt atttttgtat taactacttc atctaataac ttaaagtcat
    13321 ctttcaaaga tttgtatatt cttttataca atctatgata tatcttgtat gtatctacat
    13381 tttcacttat tggttctaat ttagttgaag tttttattat agcatcacaa gctttttgta
    13441 tattttcata tattccagct cctactaatg caagaattgc aactccaagt gctggtcctt
    13501 cacttgaatt tatagtatat acatcttgat taaacatatc tgcttgcatc tgtctccata
    13561 ctttactttt tccacctcca cctgaggctc ttacttcatt aacgtctata ttaagattct
    13621 ctattatatc catacagtct ttaagactat atgaaacacc ttccattatt gaccttgcga
    13681 tttctttttt agaatggatt ggagataatc caaagaacac acctcttgca taagggtcaa
    13741 gatgaggagt tctttctccc atcatatatg gtaagtaaag caatccatta catcctgttg
    13801 gtacttgact tacttgatta tttataatct catatacatc ttcacctaaa gaatttgaaa
    13861 cttccacttc actttgacag aaattatctt taaaccattt taatgacaat ccagctcctt
    13921 gagtaactcc cataacatgc catgtatttg gtattgcatg acagaaagta tgaactctac
    13981 cttctttatc aatttttggc tctttcgtat aagcaaatac tacacctgat gtacctatag
    14041 tagatgaaac tactccatct ttaactattc catttcctat agctcctgct gcttggtctc
    14101 cagctcctcc taccactatt gttccttctt taagccctgt caaacttgct acatctctag
    14161 tcacataacc tgtaatctct tgtgattcat aaactttacc taaaagattt ttatctatat
    14221 ttaacttttc tagtacttca tcgctccaat ctctttttct tatatcgagc aactgcatac
    14281 cacttgcatc tgatacctct gtagcatatt cacctgtcaa tttgtatctt atataatctt
    14341 ttggaagaag tattttatta gtatttatat atatatctgg ttcattgttt ctaacccata
    14401 atatctttga tgctgtaaat cctgtaagcg ctggattagc agttatttca atcagtcttt
    14461 cttcacctac taaatcagtt atctcctcac actcttttac tgttcgttgg tcacaccata
    14521 ttatagagtt tctcaatgtt ttaccttcat tgtccagcat aactagacca tgcatctgcc
    14581 ctgataatcc aattcctttt atacattcac tgcttatatt tgaactttga actacttctc
    14641 ttatagattc gactacagct ttccaccaat catttgcatc ctgctctgcc caacctgatt
    14701 tctcttgtat aagaggatat tcatatgtac aactttttat aatatttcct agtttatcaa
    14761 ataaaactgt tttagtacct gatgtaccaa tatccacacc taatacatac tccaacttca
    14821 tccctccttg aatgccactt cataactatc gttttcctat cctaattttt ttatatttta
    14881 ggtatatttt acttgcttta tttttgttag tatattaaac taacttatga actaataata
    14941 aaatatttaa ttatctttgt caatgcaaaa taacctttta tttttgtgta aaaagtatta
    15001 caatattatt ttaaggagga atatcaaatt ggttactgat aaatacacta ttcgagaaat
    15061 gaatgaaaga ttggtacttg aacaaattat aaaaaatggt cctatttcta gggcaagtat
    15121 agcttctact ataggattga ataaagcaac tatatccgct attaccaaaa aacttattga
    15181 tgaatctcta gttcatgaaa taggaattgg aaatagtact catagtggcg gaagaaaacc
    15241 catattatta gtatttaata aatgtgcagg gatttcttta agtatggaca ttggatacga
    15301 ctatatattt tcttctctat cttacttaga tggaactatt ataaattcta agaaattaac
    15361 agatatacaa gtaagtaaag acaatgttat acaattgata gatgaaatta taaatagtta
    15421 taacatatcc aaaatagaca ctccttataa ggtaattggt cttactttgg ctatacatgg
    15481 gattacttgt gaaaataaag tattgtttac tccatattat aatcttaatg agattgactt
    15541 atatagcatc cttagcaaaa agtatgactt tcctattcat atagaaaatg aagcaaactt
    15601 aacagcttta gctgagaata ctttttcaac tgtacataac tcactactta gtctcagtat
    15661 tcacagtgga tttggttcag gcattataat aaataataaa ttatatagcg gcagaaatgg
    15721 tatgagtgga gaaattggtc acactatcat catgcctaat ggtaaactat gtccttgtgg
    15781 aaatcgtggc tgtttagaac agtattgttc agaaaaaaag gtatttgaac agctttcttc
    15841 tttagaaaat attcctaaaa tagattctga tatagtaaag caactatatt atgaagataa
    15901 ccagaatgcc aaaaaagtta ttcatgagtt ttgtagttac ttaacaatag ctataaataa
    15961 tgctattaca acttatgctc ctgaaataat atatttaaat agccaaataa ttagtgatat
    16021 ccctgagata ttacagatta caaaagatat gttagttagc tcttttaaca aaggaataaa
    16081 tatagaaatt tcatcattag ggtctgaggc atctctatat ggtggctctg ccgtaaatat
    16141 caagagcttt ttgaatatac aaaatttaac tttaattaat gaaatataag ggttatgact
    16201 atatacagcc ataacccttt aaattataca ttctaaaatt agatttatta tatagtttat
    16261 gcaagattag ctatattttt taaaaattgt atatcaatta agtattaaca caagtatttt
    16321 gttttaaata ttaatataat cttcctactc atcatcagat aataaacttg cattttgttc
    16381 aaccatagaa atattatctt tacctatttt ctctaatttt gctgccaatt cctttgtatc
    16441 taaaaaagct gattcaataa ccatacctaa atttagccct gaaataattt taattcgact
    16501 gtcatcattg gctactaata aagcagcatt gcatggagtt ccacctagta aatctgccat
    16561 tataattatt tctgaacttt ccttgtacac ttctaaagct ttttttaatt tttctattgt
    16621 tccttctatt ccatcatctt gtttcatatt tacagtagga taatctatag aatcccctat
    16681 tatcattttt gcagaattta agacttcttc agccatattg ccatgactca tcaaaataat
    16741 actattcact ttaatacacc tctttaaatc tataagatgt ctacaagata agttttctta
    16801 tcatcaggcg tcattatagt tgtaatttca attccttcat ctaaaagtct tttaaatgct
    16861 tctttctctt cttcagtaac actgatatta ggtcttagaa ctgttgtatc ttttctactt
    16921 gacatatttc ctacatttat attatgtatt ttaaatcctg cctctacaaa tggaataagg
    16981 tccacaggag acttaacaat taataataca ttttgtcctt catactttcc tgcagtaata
    17041 ttttttatag cagtctcttc tgtaattata gatgacgcta tacctgcagg tgtagccatt
    17101 cttaaaactt gtttttgaac agaattgtta gctactgcat catttactac cattaatctt
    17161 gtaacaccca atttattact ccacatagtt gcaacttgac catggattaa acggtcatct
    17221 atacgaatat ttttaatacc tttcatttcc atatctaata tcatcctttc cttattttaa
    17281 aatacctaat gcatataaaa ttattgaaac aaccattact cctaaaatta agtatatagg
    17341 ttttatattc tttttaagag cccaataaac aatcacaact aaaattgcag gaactaaccc
    17401 aggcataatt tggtctaata tttcttgacc tttcattgta aaatcacctt gttgaaagtt
    17461 caaagcaaca ttagctttta caactgaagg tattaatgca cctacaacca ttaaacctaa
    17521 aatagatgta ctctcagtta aaatctttaa acttttttca attgaagata ttagcttagt
    17581 acctgatgta tagccagcat taaatagtgg tatacttaat aatttcaatg ctacactcac
    17641 tacaatccac agtaagattc caaatggact tccatctatc gccatattag ctgctatagc
    17701 tccaaaaatt gtccacggaa taacgacaaa taaactatct cctaaacctg ctactggtcc
    17761 catcaagcct gttttaattg aagcaacagc ctcttcagtc tcttcacttg ataactcctc
    17821 cattgctaca ttaataccca aaatataagg ggcaacccaa ggattagtgt taaaaaattg
    17881 taaatgattt aatacagcac tttgtaactt ttctggatta tctttgtata ctacttttaa
    17941 aaaaggtagt actgcataac agtatgccaa ggcctgcatc ttctcataat ttgaagaaac
    18001 agaactagta aaaaaccagc gccataagac actttttttc accttttcat ttgatttatt
    18061 taatgatacc ttatcactca tcttcgtcca tacctcctac tacagtatat tgttgttctt
    18121 gattttccaa attctttttg taaattataa gtgcaattgc tccaccaatt aaagctacac
    18181 ctaataatgg tactttcata tatactgcta aaacaaaacc aattactaag taactaaaat
    18241 actccttagc aggaagatat ctaagtaata atcctatccc tactgctgga agtagtcctc
    18301 cagctactgt taaaccacct gttaaccatt caggtgtata tttcaaaatg gtattaatta
    18361 aagaaggtcc aaatattaca gatagagcaa ctggaatacc tgttgtagct gctgttaata
    18421 gtactcctaa catttgcata taggttatat ttttataatt tcctttttta gctccatctt
    18481 ctgctttatg aactagccat ataccaatag tatttctaag aacatctaat tgtaccatca
    18541 ataaagcaac tggaatacct atagagattc ctacagatgc ttcttgctta gtcgtaatag
    18601 ttataaatgt ggctactata cttgctgttt gataatcagg tattgatgct ccaccaaagt
    18661 tactaatacc taatgataat agctgtaatg tacctccaat atataaccct gtatttatat
    18721 ctcctaagat aagaccagta ataaaacctg ctgttactgg ttgatatgtt ccaaacattg
    18781 ttgaattttt ttcatagttt ataaaaaatc catatagtac gattaataat aattgccata
    18841 attgtggctc cattatgatt tcacctctcc caaaatattt tttagttctt taaaattata
    18901 actggaatct tgctttagga aaataggaat ttcttccagc gggcattcaa cttcataagt
    18961 tttcccacct tcataactaa ctccactaaa tatattttcc catgtatgtc cttttggtaa
    19021 atatactttt ctacttctat ctttataatt cattattgga gctaccaaaa tttcatctcc
    19081 aagcatatat gtattatcca cctcccaagt tttatcatct tcaggaaatt cataaaataa
    19141 agttctcata actggtgttc catcttcatg cgcttctttc atcagttttt ttaaataagt
    19201 ttttaagctc tctctaattt ttatgaattt tgtaagaatt acttcaactt cttcaccata
    19261 agaccatatt tcattaggag ctccagttac cattgagcct ccacctttat ttgataatgg
    19321 tttactatga ggcaatctat ctccatgcat tctcaaaatt ggtgaaaaag tagcatattg
    19381 gaaccatctt accataagtt ctctaaattc tgggtcttta ggatttcctc catggaaacc
    19441 tccaatatca gtagtccacc atggtatacc tgccagtccc atatttaacc ctgtatttac
    19501 ttgattatta aaagcctcaa aactagaatc aatgtcacca gaccatacta aagctccata
    19561 cttttgagct cctgcccaag ctcctcttac aagagttaca acagactcta tgtcattatg
    19621 taaaccatca taaatcattt ttaaataatc aataggataa atattaccac aacttaaaac
    19681 atttcctttt ttatatctat agttatcaaa atcatacaca gaatagccag gttcagctac
    19741 atctaaccaa taatagtcaa tcccaagttg tttataattt ttatctattc tatcccaaac
    19801 atattctctt gcattttcat ttgtcatatc tacaaagact gtatttcctt gaatttgcat
    19861 tgtcattcta acaccacgat taacattgac taaatagcca ttttctaaat aatcattgta
    19921 gttttctgca tcactttgaa ctgtaggcca tacagatact attggttcta ctgaatactc
    19981 ttctttcaat tttttaacta attctttagg atttttccaa taatctaagt caaatttata
    20041 ttcaccttgc ttaggccaat gaaaatagtc aattgcaatt gatgaaagct taattcctct
    20101 tttagaatat tccttcacaa catctaaaac ttcttctgaa gttctatatc ttagtttact
    20161 ttgccatagt cctaaaaggt tttcaggcat cataggaaca gttccagtaa cttgagaata
    20221 atttttattt aactcttttg ggctttctcc acaagtaatc caataatcta taaaatttgt
    20281 agaaaacatc ttccattctg tcatattttt agcaaaagat acttttccaa ttcccggatt
    20341 attccataaa agtccataac ctagactaga aatataaaaa ggaactgata tttgagaatt
    20401 tctttgggct aactccaaaa cagtattctt taaatctaaa aatttatgtt gatattgtcc
    20461 cattccaaaa atcttttcag aaggctcaga ttcaaatcta gtagttactt caaattctcc
    20521 actatagctt ggtcgatatt ctcttgattt taactttaga gtagagttaa agtctttggt
    20581 aatctcaatt gtccctacat cttctccacc atcatcatgt tttacagctc ttaatcgaat
    20641 atattctttc aataatattt catttttatc attataaaaa gtaattctat ctctatggtc
    20701 taagacagct tttatctttc catttttaat gattgcactt ccatcttcat ttttattaat
    20761 tgtaatatca tctttattta acttaggttt ttcgttaaga gcatagtttt catcaacaaa
    20821 attttggtct acaaacgacc taatacgtaa agagtttttt ccccatgctt ctattcgcaa
    20881 tagttcattg tcaaatcttt tttcaatata gtcttcagat atttttatca cggctacctc
    20941 ctaaatcaat ggtataatat tctatctttg tgtagcttaa gttaaataat gtcgtttagt
    21001 atcgttattt ataaattagg ttttatatat aattcatttg tacattaaac taacttacca
    21061 ataatatact ttatttgact ttgtttgtca atcgctttcc ggattttagt aaaattttct
    21121 aatatgttgc ttgaaagtcg ttcttaatat cttttattta tacttttaat atctgtgatg
    21181 atacctatat acatctagtt ttttttaaac acatacagga ataaaatttg cctattatgt
    21241 agataatatt ggacttgaaa atatgtatag tgaccttata aatacagata ataatatatt
    21301 agatattgtt caaaacaatg gattttataa ttataaataa tttatgaaat tatttaagaa
    21361 aatatataat tgtataccaa gtcaaaaacg acaacaaata aataaaacta aaaaagatgt
    21421 tgtctcaaaa tatagataaa tttatttttc attgctaaaa tgattaaaat atatttgatt
    21481 taatatactt gatttaatat aaaaaaagag tgtctcataa actaaaaagt tcacttttag
    21541 acactccttt atatatatta tcaattcatt tattacctct ctacattttc tttcagctag
    21601 tagatacatc tttataagta ctaattactc cacttaaaaa tgattcaggt actgtccatc
    21661 agtcatattt ttctgccaaa tataatggtg tttcataatg agacaataaa tatttatatc
    21721 tttacttatc ttctctaagc tcgtcaaatc caactatagg tatctctaaa ttatattctt
    21781 taagtagctt ttctagctct tctataaaaa tacctttccc atccaccatt tgtatatcat
    21841 ttattttatc catcaattca ttgcaaccac ttagctcacc aaaccaacgt ttagaactac
    21901 atgtcttatt ataaccacaa cttggactac catctattgc aatgatactt tttatatcat
    21961 aatcattttt taaatattca ttaaactgcc ttacatgagg tattaacatt tgcctacatt
    22021 gttctctaaa aaacaaattg tcaaactgtt cttttacatg tccccaccta tttattccat
    22081 acataatcat ttctgggcat ggtagttgaa ttattccata ccctttattt attaaattta
    22141 tcgctatatt ttgtgctccc ttaaattctg acaatccctc tactttagaa ttacaattta
    22201 atatacaatg acatactact acaatctctc tatttctctt cataataatc tccttagtta
    22261 ttcttaattt taatgatact acatattttg ataattatat tatttatttg ttctatattt
    22321 ccaatattct atatagaaat atacaattag taatctttta taatctatac tatgataaat
    22381 gtatatattt ttttaggagg tattataatt ggatactaaa aaagtcacat tttcttcaat
    22441 atgcatagtt attaatattg tcttaggtat ggttatatct atgataaata taccattatt
    22501 atttttagat acagttggta ctgtacttgg ggctgtcgta ttaggtcctt tttggggtgc
    22561 tcttattggt gggtgtacaa acttagtttt aggtgttata tcaggtccta caaatatacc
    22621 ttttgctctt gtaaatattg ttcttggtct tattgttgga tatatttcta agaaaaaagg
    22681 ttttggttat aaagaagcta ttattacagg agtaatttta tctattgttt gtcctttaat
    22741 tggtactcct atttcagttt tattgtttgg tggtttaagt ggaagtggtg ctgatttatt
    22801 ggttggtttt ttagttcagt ctggtcagaa gatatttact gctgcgttct ttccaaggat
    22861 tcttagtaat atagtagaca aacctttgtc ttgtattatg gtggtatatt ttataagtaa
    22921 aatgcctaaa ggttttataa aacaatatag taaaaatact tctagttcat attaataaat
    22981 catttattat gtaagattta ttactcttta aaatgctata tactcatttt tatataacat
    23041 ttttattaat aatttaatta gatagtattt gtattatttt aattaaacgt aacctcttga
    23101 tataaatttg agctataaat ctatatcaag aggtgctatt ttatttttaa tataattaat
    23161 attctattac gcaaaaaatg catatatttc attattttat ggaaaatatt acttcattta
    23221 actctatatc aaactcatta agtaaaaatc taattatatt taatttagcc tctgtattta
    23281 aatttgtttc taaatatata ttttctgata ttttaatacc ttttcttaat ttatatacat
    23341 ctctagactt aatattatct ataaaatctc ttttataagt cttgtctaca atactgttaa
    23401 accttacaga atccaaatca tataaagtaa gagcaacttg ttcataaaat tctgtccaat
    23461 ttttagcctt atatgactct cctaatatac taaatgatac tatactttca tttgtaaaat
    23521 taacatcatc atcctctaac gtaaatatat tttcactgtt acttggaggt tcatatgtaa
    23581 cacttggata tttccaaatt tctacagctc tatctagtaa tatcttagtt ctatttttta
    23641 tttcttcttc attccattta tctatagatg aaatatattt atttagataa agcctactat
    23701 ctttaaatcc atcttccatt tctttctttt cttcaaatgt tttattactt agttttgaat
    23761 tatatcctgt caaagttaaa tttccaattg tatgtaaata tttatcatgt atctccttat
    23821 agttatctcc tagtgattcc ttccatttat ttgttaatgt ctgtggcatt atatgctcta
    23881 tatttagcat attattattg attaaatttt ctaaatcaac aatttcttta ttattataat
    23941 tctctaacct ctcaagtata tataacttat tctttgactt agtattatat atatctttag
    24001 tttcaaaagc atttgaaaat tcactgtcag taggaaatct ttgagatgat ttcttattta
    24061 taaaaatata tcttaatata ttagaatagt tttctttata atcactaaat tttttaattt
    24121 cccttcctaa gttcataaat actttattca gtgcattcgg aggtacatca catactattc
    24181 ttcttaatat aaaacattct actaaattaa gggattctac aagtactttt ttatttatta
    24241 taccttcatt ataatcatta aataattcta atataaacgg atatgataca aatatttcaa
    24301 gtttgtttat ccttttcaaa actttgttta catcattatc atcagtacat gaataagata
    24361 tctctctata atagttagaa aatttaagta aatcttttaa taaaatttct atatctatac
    24421 cattatctga tacatacctt ttaaaactaa aatatacttt atttttattc ggtatacttc
    24481 tctcctttat agtcaaatag tctcttataa agctacttac gttgctactt gtattttttt
    24541 caattttata ccaataatta ttataaaatt cctcttgtaa cttagagctt ctacccatta
    24601 acacaaagtt tcttactcta tcagcttcac ttaaatctaa gccagttgaa tttagacttt
    24661 caaagattaa ttgagggtca tcttcaccat tttttaactc tatttctact atagttaatt
    24721 tctgtatagc atcaaacaaa tcatctattg atatattatc ttcttttatt ctattataaa
    24781 aatataaata atttgaaact atattagaat ttctatctgt attcatttct tcttcaaata
    24841 gttcatagta agcttttcta tcttctgcta ttggttttaa ctttattttt ccatctttat
    24901 ctcttgagtg cttgtttata agataacact ccttaatctg ttcttctgtt gtaataattc
    24961 cttttgattc tccaaactct tttattattt gacacatggc aatcaataat attgacacag
    25021 tagtcactct ttgttgtcca tctattatta aatattcttt tcccatacta tatatagata
    25081 caactgaccc taagaaatga gttctaaatc cagaatctat tatatcctct aaatcatcaa
    25141 acaatctttt acattgttct ttcttccagt tataatttct ttgatataca ggcactacaa
    25201 aacttttgtt tgacccctct aaataactaa gaaattcaat ttctcctgct ttcatattaa
    25261 ctccccctat aagttaaata aattccattc ataactccat gtatctatat attatatcaa
    25321 aattttgact cataaatgta tatgaaagcc ttattatata cctttttata cctattttta
    25381 ttttattaaa gtaaatacat gattattcta taaaatatac aaatacttaa ttttaaaaat
    25441 tattatttaa tcttcttcat gagtaattca tagttattta tcaattaaaa agagatattg
    25501 cataattatt caatacctct ctttgtatat ttacattatc catatctagt tatctatttc
    25561 aacccaatat tagactacct actaataaat ttattctcta cataattaac aataattttg
    25621 cactaaataa catcattagc ctatctctga tgcattttct ttaattttat ttatagtttt
    25681 aatcaattta tttctctctt caatatgatt ttcaaataaa tcaattatac ttccaacagt
    25741 tctaaatggg tcttccatca acactttgtt atcctctata aatccattct tgactacata
    25801 atcgactata agtttaacaa aatgtatttg cttagtatta aaactcttat tatttaaaaa
    25861 ctctgaaaaa gcttcatttg cagcttctct atccaatccc actagttttc tcactaattg
    25921 acttactgga gtatctccaa actccttttt ataatcatca caattaccta actgctttaa
    25981 gagtatttcc tccaacgttt caacatcttg ctttgttatc tttttattat ttcttaattt
    26041 aaatattgct aattcatctt tatggctatt taaataatac tctaccttct ttttataatt
    26101 cttaagattg tttgcattat atacagaatc atttctatct tctgctacaa tcatatcttc
    26161 aaaacttgta taatatatct tttgactaga cttttcaaga tacttaataa gctctcttaa
    26221 tgatatacgt acttcttcta cctcaaatat atctgaatct tcccaaaaat cctctgtcat
    26281 tgctttttca atagtatatt tttgttgctg gatctgtggt atagtcccaa gtttcgaaag
    26341 tttttctgct gtaaccatta catgccttat ccctttactt gcattattat tttgtaagta
    26401 tgcaagttgt atattataca taactacatc aaatctttta gccaattcat catcactata
    26461 tgaagttatg agtggtgata tatattctct tatctcattt tgtgctactg ctccaatatt
    26521 gctccaaaca ttttcatttt tatacttatg tacataactt aaattcatcc taactatata
    26581 actatcctca tttaaagctt ttacagaagt tataagttct tctaacaatt cttttctaag
    26641 ttctgcatac ttaccttcta tatatttaat atcttgaagt tctttaacca tacttacctt
    26701 tatattaaat aatttttcag taagtgtttc tgctttattt ccctcaaatc ctttaggatt
    26761 aaatttaaaa aattcaaaat tattacaaaa atcaaatatt aagaatcttt ccttatccat
    26821 atcaacacct aataaatctg gacataatct agttcctcta cctatcattt gccaaaactt
    26881 agtcttactt ctcactttct tgaaaaaaac aagatttagg atttcaggta tgtcaattcc
    26941 tgtatcaagc atgtctactg aaactgctat ttgaggtaat ttattcttat cagaaaaatc
    27001 atctattata gaatctacat aatttatact atagtcaact gcttttacaa agttcccacc
    27061 atactttgga tacagtttat taaatctttc aactattgcc ctagcatgct ttgtattctt
    27121 tgcaaaaatt atagtcttac caatcttttc ttctccttct attttcaacc cctctgtcat
    27181 tagcttattt aaaactaaat ctattgtgtc agaattaaat agccactcat ttactgcatt
    27241 attccctatt tcgtctccaa tactctcatc atcattaaat gtattttcat attcttcctt
    27301 ctcttcatca gaaagctcat catacttaat tccatcttcc ataatcttag tcttaaactc
    27361 aatactagta taatctacta aatatccatc ctcgactgct ttattatact cataagcata
    27421 tgtaggtact ccattttcca tgtcaaaaac tgaataagta tttttatcta tctcatcctt
    27481 tggtgtagca gtaagaccca ttaagtaaga atcaaaataa tcaaagattg acttatactt
    27541 tttataaata cttctatgcg attcatctat tataattaaa tcaaaatgac caggtgtaaa
    27601 caatctcttt ccatccttag acttcgtatc atcaattgca ttcatcattg ttggatatgt
    27661 agaaaatatc atcctagcat cttctgggct gtctttgcta tccaataaat tacataacgc
    27721 caagttaggt aatagcttag taaaactttt tttagcttgc tttaccaatg cttttctatc
    27781 tgctagaaat aatatatttt taatccaatt atgtcttgaa agtacatcaa ctatagatat
    27841 agcaattctt gtttttcctg taccagtagc acataccaaa agagttttgc gttgcttatt
    27901 ttcaagagcc tcacatacag ccctaatagc ttctttttga taatatctat tggatatttc
    27961 atcatttatt gttacagaac ttaaagactt cttactcttt cttctatcta tcataagttg
    28021 taattctgat tttttataaa atccataaac ttttctatct gaatagtcat cccaaatata
    28081 catatcaaaa ccattcgtca aaaatattac tggtctttga ctatatctct tttccaaaca
    28141 atctgcatat aatttagctt gttgttgacc aattttcggg tctttgctcg ttcttttcgc
    28201 ctcaactact gctaatggct taccattttc accataaaga acataatcta catatcctac
    28261 accactttca ttaggcatac cttgtacttc tatttcctcc cctatatctt ttccaaattc
    28321 ccaacctgca agctttaatt ctatatttat atatcttttt cttgtctcaa attcgtttac
    28381 ttcgtctatc ttgaaatcgt agtttacagt attaactttt ctcttttcag ttagtgactt
    28441 tcttaatttc tcattttctt ttattacttc ttcaagcttt ttatcttttg aacttaatct
    28501 ctcataaaga tttttaagct cgtctggtct cgtccttttc tcttccccat gaagtaataa
    28561 atcttcatta aagtcttctg ctgtatattc atctgaataa caatagtcta tccaactaac
    28621 aaattgatgc aaattgttaa gtgaaagtat tgcttctcct ctctctatgt tcgcactagt
    28681 atgtacagat aaattaccta atttaactat atattttaat aaaggtaaca tatcatagtc
    28741 tataagttct ataaaactat tattatgaat caacgtacta agattatctt gataaggcaa
    28801 tactaaatca ctatcattag catatagcca ttttactgca agctctaaag ctcttcttgt
    28861 tatagttgca caagtagatg ggctaactag tatacttttt tctgcttcta agcaagcagt
    28921 attaaaacta ttgaaatttt tcttcttttg taaaaagtca aaattaccca aggcttttct
    28981 cccccttttg tcccttagtt aaaataaatc tctctaaaaa ctatttctta cagctgtttc
    29041 ttatactaac aaatacttct tcattttctg taaacaattc gtacatatct tcaaactgtg
    29101 gtatatcata tccatgagaa tcatcatttc tcaattttct aagtgtttct ataactatag
    29161 aagaattttt aggtaataaa ttgtgatact cacaagctaa aaggtagtca taaaagtttt
    29221 tagattttat gtttaaatca attgatttat aatacaacat tgcttttgtt aattctaaaa
    29281 atgagaccat atagctttta aaaccttctt ttatgtatct agcccataac tcaggcattt
    29341 cttccttatc atataagatt ttgaagtttt gcaggtcata taagcaagaa ccaaaatccc
    29401 tatttacttc ttctattgag ttccaagata atttgtttct aattaccata catgtccctc
    29461 caatctaaga tatctattac atcactgttt tcaaaataga atttatttat ataatcctta
    29521 aaatctttag attcataaat aacttcacct tctaaaatag cttttttaag tgctattgta
    29581 taattatctg taacaatttt taggtctaca tcaatgtcta atatatttct tagagcttct
    29641 gtcttagttg caagttctgt aattggtact tttttgatag gaatccaagc tatgtctata
    29701 tcgctttctg atgtatgttt atctgttcca taactaccaa atattatgat tgctagagta
    29761 ttttcttttg gaaatacatt tattattgag tttttaatct catttggtat atttctcatt
    29821 taatcacctc ttgtttatta ggtaatattg cttaattgaa tttatatcca taatctttta
    29881 atctatttat acatatttct ctttcttcat cagtaaacaa ctctttatac ttttctttta
    29941 atactaatac ttctaatgat aaatctaatc tcttacactc ccatagcctt gtaaatcctt
    30001 cagtagctcc ttctttattg attaattctt tagcagctaa taaggctcct ttgttattta
    30061 tcagttgtaa aaatcttgtt ggcttatagt tacattgtaa atcagcttct ttatagatat
    30121 ttaccatgtc atgatataaa tctagctcta tttgattcat agattttatt cccccatttt
    30181 aatacttgta tttcagttga acaattctcc tttaaaagct ttttgcatta atgagttaaa
    30241 gttatcttct agttctttta agctagtttc catttcaaac ttcaaagagt ttacttgttt
    30301 aacgaagttg gcaaattgat tttgaagtct aataggaggt acaataatat ttatttttct
    30361 aacagcatct ttagttatcg tttttgttac agctcctctt actttctctt ttattttact
    30421 ttccatatag gatgttagta aaaggttata taagaatata tgattcactt tatcttcatt
    30481 aaatctaagc ataaattgat tcatagctaa agatactggt ttatttaaaa atggcattaa
    30541 ataaacttta cctgcactac ctatcttatt tattataacc tcaccaccaa acacctttga
    30601 tttttcaaga taattatatg catgttcatc gatatactta acaccttttt caaagttatt
    30661 attttctaaa tctgtagttc ttaccattaa ggcataacct tttgtatcta aaagagttac
    30721 attatctctt aaggtttcat aactaccatt tgaatgataa tcagttaaaa catctaaata
    30781 tttccctatt tctgaaattt cccagttttt ttcgttcttt gatggggtac caaacatctc
    30841 aataaatctt gatttaacta attcatccaa tacttctatt tgctcttttc ttttatctat
    30901 taactcttga gccttgtcta aaacacgcac taccttttct tgcactttta aattatacat
    30961 gcgtattttt atattagcta atccttcatt atttaaagct gattgtgtag cacctttact
    31021 taacctattt ttttgattta gaaaataact actatttaag taatggtata aatattgagg
    31081 caatatttct tttgatggtt ttaaaacata aaagccagtc gatactatta tatttttatt
    31141 agttttatta ataactagtg ttttctttgt gtccttcatt tttgcaaata taacttctcc
    31201 tatatttaca gtttgatttg ctctagaagg tttattttca taagtaacta tttctagatt
    31261 gcttatcttg ttaaaatcta agtttccagt actaagatat gagcgttctc catcaaactt
    31321 ttttacattt tccctgctta tactaacaac atctaaaatt tttgcttcat caaccatact
    31381 ctacacctct aacattcttt ctagttcctc taatccatcc actatctctt tctctaacac
    31441 cttaacccta cctataataa cactaggagc atcataaact acttcctcat acactacctc
    31501 tttatactta ttaatagata aatcataccc attctctctt atctcttcca catcaacaaa
    31561 aaagctttgc tctgttcttt ttctatctac ttccccatca agattatcaa atctcttaat
    31621 aatatcctct atatcactat cctttatctc atttctctta tcatccaaag aataaccatc
    31681 agctttcata tcatagaacc ataccttatc agttccacct gtccctgttt tagtaaatat
    31741 cattatagct gtagataccc ctgcatatgg cttaaataca ccacttggca ttgatattat
    31801 agcttccaat ttatgattat caactatttc ttttcttatc gatttatgtc cacctgttga
    31861 accaaacaat actccatctg gcactataca tgcgcatctt ccaccaattt ttaaaactct
    31921 taaaaaaagt gccaagaata ataactctgt tttctttgtc ttagtcactt ttaataaatc
    31981 tgctgacact gcttcataat ctaaacttcc cttaaaagga ggatttgcta gcaccaatgt
    32041 aaacttatct ttatctgtat taacctccga taaagaatcc ttatactcta tattaggatt
    32101 gtcaacgcca tgcaacatca tattcatagc acctattcta agcatagttc tatccatatc
    32161 aaatccatag aacataccat tattaaaatg ctccttcagc ccttgcacta agaaaagact
    32221 tgaatgatta tcccttaagt actgttgtga tgatactaaa aaacctgccg aacctgctgc
    32281 cgggtctact ataatatctt ctggtgttgg tttaataagt cttactatca tatctattat
    32341 gtgtcttgga gtcctaaatt gaccattagt acctgctgta gccactttgg ataataaata
    32401 ctcatacaaa tctccctttg tatctctatc cttacttaat tctaaattac taataccatc
    32461 aactatttta gataacataa gaggcgtagg aattttaaat attgcatctc ccatgtactt
    32521 tgcataagct gattctttat ttccatgtaa tttttttatg aaaggaaaca cttcattttg
    32581 tactatttca tacatttttt ctgagtttcc ttcattagaa aacttactcc atcttaaatg
    32641 ttgatgttca tcatcaaata tcttttcaaa agaaacaccc aaaagagttg cttctgcttc
    32701 attttttgtt tctacttcat ccaatccttt aataaataaa agatatgtaa attgttctat
    32761 aacttctaat ggattagtta atccaccagt ccaaaatgtc tcccaaatct tgtctacttt
    32821 actccttaat tctcctgtaa tcatatatga ctttccctcc agtgttcata aaataattta
    32881 aatatatatt ttagtcgtac gccttaatag ttagtgtctt atgttaaatc caagttttta
    32941 ctacacaatt attaaattat ttttcccatt tacatatttt agctaaaaaa ctaatgcaat
    33001 actcttattt tatgattatt atatatacca tacaaaatta ttttatcata tatccatcat
    33061 agaagtcatc tacctggata actcttacca tattataact ttttaatatt aatttaaact
    33121 tagaaagaaa ttttttattt aattatatca tcttccttat ttcaaatttc tcaactacaa
    33181 tcctaatcaa aaaataaaat aagatgctgt ctcaaaatag atataatctt cttttttatt
    33241 gtcagaatga tgaaaatata tttgatttca cacaaaaaag agtgcctcat gaactaaaaa
    33301 gttcattttg agacacccct taatttaatc ttacttattc tttcccttta ttattaacac
    33361 ttcactgtcc ctattgcttt tcctacacat ccacaaactt ccatcctact cttaacaaaa
    33421 tctttaactg actctaaacc ttttttacca tatttcttag ggtcaattac ctctgggtca
    33481 gaattaagca catccttaac cccattgcta taagcaatcc ttaattcagt tgcataatta
    33541 actttgcaaa tacctctttt tatagattcc ttaataacct catctggtat tccagaccca
    33601 ccatgtaata ctaatggaac tgatacaacc tctctaattt cagataatct atctaaatca
    33661 agctttggct ctcccttata tagtccatgt gccgtaccta ttgctactgc caaagatgat
    33721 acccctgttc tctctacaaa ctcttttgct tctaatgggt ctgtatatgc tccaccatca
    33781 ccaccatcta aatcgtcttc cttacctcca actttgccta attcagcctc aacaggtata
    33841 tctgaaggca tacatgcatc tacaacagat ttagatattg aaatattatc ttcgtaactt
    33901 tcatgagaac catcaatcat aatagaagta tatccaactc taagtgcctg catagctagt
    33961 ccaaagcttg aaccatggtc taagtgcatt gccactggaa cacttgcctt ctctgatgct
    34021 acctttacat tcgctaaata ataatctagc cctgcatact ttattgttga aggtgttgtt
    34081 tgcaatatta ctggtgattt aagttcttca gcagcctcta ttactgccat aaccatctcc
    34141 atattttcaa cattaaatgc acctactgca taatgccctt cttgtgcatc taataatatt
    34201 tgtttagttg tcactaatgc cataatttct cctcctatat gtttatactg atttgatttc
    34261 ttcgttattt aattttgata ctatttctct acttgttttg ctatttaata actcatttaa
    34321 aaattcttca ttcattaatt ttgaagatag ttgagataat gtttttaagt gtaaattatc
    34381 accattatct gtagctgata tcataaatat aatatgtgcc aagcttccat cttctgattc
    34441 aaagtcaaac ccttcttttg atacacctac tgctacacgt ggtaccttta ctgcatctgt
    34501 ttttgcatgt ggtatcgcta tccccatacc aaaacctgta gatgatattt cttctctttt
    34561 aaggatttct tctttaaact tctttttatc taaaagaaca ccagatttat caagtattcc
    34621 aatcatttca tcaattacat ctgttttatt ttttccctta acatctaaaa ttactgtttc
    34681 ttcgctatat aaagagatat tattagatac gttttccgat tctgcacttt catttttatg
    34741 tccatctttt gttgcatttg tattttctat agtatttttc tcatttacta ctttaggtgt
    34801 aaccatcata taaagtacag cacctactaa tgaacctaca aatatacacc ctacccataa
    34861 aagtggctta ttgactaatc ctagaaccaa aaaccctcca tgtggagctg gtacttctat
    34921 tttcattata taagttaaaa ttgatgatat tgatgatgca atcataagta ctggcaacac
    34981 ttttaatgga ttttttgctg caaaaggtat tgccccctct gttatatgag tactacctag
    35041 tatataattg attaatcctg ctgctctatc ttcttctgta aatctatttt taaataaagt
    35101 agatgctatt gctattacca atggtggtgt tatacaagct gcagatacac ctgccataaa
    35161 taaataattt ccctgtccta gaagcatagt tccagttaca tatgctgctt tatttactgg
    35221 acctcccata tcaaatgctg acatacatcc tattactatt cccaaaatta taggacttga
    35281 attttgtaac cccgatagaa agttcatcat agcattgtta agtgctgaac atggtccacc
    35341 aagtaacaac attgctacac ccacaagacc tacgctgaca ataggaatta taaaaatcga
    35401 tttaagacct tctaattgtc gtggtaattt tcttaacaaa tgtacaaata gtttaactaa
    35461 gtaacctgct aaaaatcctg atatgattcc acctaaaaac cccgaacctg tatcaaatga
    35521 tatcattcct ccaacaagtc ctgccaacat accagctctt cccgatatag attgagctat
    35581 aaatgctgcc aatacaggaa ccatcaagtt catagaatat ccaccaacag attttaatgt
    35641 agctgctatt tgattatatt gttctgaatt tgggtcaaat gaataaatcc cccataaaaa
    35701 tgatatagca gtaagtactc cacctgctac tactaaaggt aacatgtgag atacaccatt
    35761 cattaagtct ttatacaact tttgaccaaa cgataaattt ccttcttctg caattttact
    35821 gcttgataag tttccttttc taatagctac ttttccatca atacaccttt gtataagttc
    35881 attagcctta tgtatacctt ctttgacagg tacttctatt accggctttc cattgaatct
    35941 gtccatatca acatttttat cacaagcaac tattattgca tctgccattt cgatatcttt
    36001 tgctgttaac ttattttcta cacctatagc tccattcgtc tcaactttta tgtcacaccc
    36061 taattccctt gctgaatttt ttaatgcttc ttctgccata aaagtatgag ctattccagt
    36121 tggacaacct gttaccccta gaatcatttt ctttcccatc tttataccac ctttctgact
    36181 cttatatttt ttatataatc ttccacattc tccaaattac ctataccatt actttcagcc
    36241 acacttgctc ctgttgctgc tgaccttttt aatgcttctt caatatgttc tggattttcc
    36301 aaccatatac ttaaaaaagc agctaatgca gaatcaccag cacatgcaga gctgactact
    36361 gtaactggtt gagcactagc ataataaatt gattttccat tataaaaata agaacctttt
    36421 tctcctaaag ttagcaatat attttgagca ccttttaaat gcagtaattt aagtacatcc
    36481 ttaatatctt cttcatctct tacaataatt ccaaatatat ctttgatttc ttcatcattt
    36541 ggttttatca aatatggatt atactgtagt aactccttta attttggtga acttatatcg
    36601 agtattgtct ttatactttt atttttacaa attttaaata tatcctcata gtaattatca
    36661 tcaataccag gaggtagact tccacttatt gataagtaac ttatatctgc taatgactgt
    36721 atcttatcta gcatatctag tttttgtttt tcgtttacat atgacccaga gtttacaaat
    36781 ttaaattcat cacttccatc atttaaaaat atatttatac gagttgtatc ttctacccaa
    36841 gtaggtaata cgtcaaatcc tctcttttcg ctttcttcaa ctatatattt tcctgaaaat
    36901 cctccaaaaa atcctaatat cttagattct ataccataat gacctagtac aaaagttaca
    36961 tttaatccct ttccattagg agaatataca gcatttgatg tacgatttac taattttctc
    37021 tttatcccat tagtagatat attcatatct attgcaggat tcgtagttaa tgtatatatc
    37081 attgtcttcc cccttgcttg tgctgtgtat gttttcatta taataaaaaa gtctatgaaa
    37141 atcttttcac aaatagaaaa tcgattttca tagacttttc aatttaattt tcagaattat
    37201 tatatctgac catataatct ataattatcc tagatataat atacaagggc aatctggaag
    37261 atacttcata ttcagaaatt gatatatgtt catgtttgta tcctacaata tataaatccg
    37321 aatactttat aagttttgca ctttcactac aacagcaagt tataacctta gctccaacta
    37381 aattggcatt ctgtgctgat tctacaagct ctatggtatt tccattcaat gaaaatatga
    37441 ttataagttc gtcttttttt attgatttag attttatttt cattatgttg gggtcattta
    37501 taaaaacaac attaaaatca agtaattgca atttaagact aaactctttt gctacatatt
    37561 cagtaagccc tcttgccaaa atatatatct tactagattt atttatagtt tttattatat
    37621 tgattagtgt agttaaagat atttgttcta taactctctg tgcttctata aaggatttat
    37681 ttattatatc ccccatattt tgtatatcat cattttcatc ttttactgtt aatctatacc
    37741 taagttcatt gaatccattt aaaccacatt ttctaattgc tcttgaaacg gttgctggag
    37801 atgaatatgt ttcatgagca atctctacta tagataattc tggtaatatt gattcatttt
    37861 catttataaa tttaattatt tcaagttccg tcttcgttag attttcagaa ttacttttgt
    37921 ttagctcaat tatcatatgt tatctcccct tatataagtt ttatttgtct aaatttatcg
    37981 agtaatataa tatattacct cttaactact tatttttata tacaattaag atttagtcaa
    38041 tgtctatata tgtaaattta taatataata attttatgct tcaattaatt atgtaattat
    38101 tagagtattt tgtaataaaa attggagtta ctcaattact gaaactgagt aactccaatt
    38161 cttacagcct caaattaact cgaattattg aaaatgattc ataaacatta tcctatactc
    38221 tcacttatca atttatatac ttgagtaata ccttttttta ttaacagaaa tcatcttact
    38281 aacaattgaa attgtagttc cagtacataa tattgaaaat atagttccaa accctaaatt
    38341 tccgcctaaa tatacaccta aaattaaaaa tattaaatct gctatagttt ttaaaacttt
    38401 atatggatac ttaaatttgc cctcaatata gattaatata gcatctaaag ggctcattcc
    38461 aatgtcaaat tttataagca ttgcaaatcc actacacata agtatatgtc caattataaa
    38521 gctcattatt ctaatgataa tccctaaact acttatatta aaatctaata ataaatatat
    38581 atacaaatct ataaatattc ctgctactaa tacataaaca cagcttccta atcccaaact
    38641 ttttttattc aataacaatg ctataagtaa tagagttcca ttatatatta gccctgctat
    38701 tgtataactt atacctaact ttatatgtat accctcattt aaaagtgtaa ttgcatcact
    38761 accaaggctt gactgaagta taagagatat accacatccc agtatattta taattaaaat
    38821 tacaaatata aattttttta tgacattcat aattaataaa ccaccttatt tataattata
    38881 atccctctta taaaactgta ttaataacat agtatttata agagggatta tttatattta
    38941 aaaactatct actttttctt ttaattcttc tccattagtt cttattacat ttttatacca
    39001 ataaaaacta tcttttttgt aacgttcttt gcttccattt ccattgttat ctatatctac
    39061 atatataaat ccatatcttt tttccatttc ttgagatgaa caacttacta tatctattgg
    39121 cgaccacata caataagcta atatttcggt tccatcagct atagcttcac ctatttgttc
    39181 tatatgtttt cctaaataat ctattctata actatcatga acttttccat cttcaaattt
    39241 atcatgcatt ccaaatccat tttcagctat cattattggc ttttgatatc tatcataata
    39301 ttgagataaa gtattgtata atcctttagg gtcaattgcc cacccccaag gatttgcttt
    39361 taagtattga ttttttacat tatcaattgg attcatggta tttttacttg ctgatactgt
    39421 tgtagagtaa tagtatgata gagctagaaa atccatagta ttctctttta acactttatc
    39481 atcaccttct tccataacta tatttatgtt attttctttg aagaatctct taatataagc
    39541 tggatactcc cctctaaaac ttacatcagt aaaaaaatat tgtattctat ttctttttaa
    39601 agcaaatact acattttcag ggtcgcaatc ttctggataa gatgtacaat ctgccatcat
    39661 catccctatc atcatatctg aatttatttc ttttccaagc ttcttagtta aagctgtagc
    39721 catgaattga tgatgaatag cttgatattt ggcctcttct acatcatcta cctggtctac
    39781 acaaaatgca actgaattaa atgattcatg atacattaaa tttatttggt ttataactat
    39841 ccaatattta actttatctt tgtatctatt taatattaat tctgcatatc taacaaagaa
    39901 atccactact tttctattag accatccttt atattttaat actaaattca aaggagtctc
    39961 ataatggagt agtgttacaa taggttccat accattatct attacttcat ttataagatt
    40021 atcataaaac tttaatccct cttcattagg ctctaaatca tctccatttg gaaatattct
    40081 agcccaattt attgaaaatc taaaacactt taaccctagt tctttcaaaa gctttaaatc
    40141 ttctttgtat gtatgataaa agtctattcc attacgctta ggataatacc cattattatc
    40201 tttcatagca aactttattt tatctacagt catatcagac tcgtaatgtt tagatatatc
    40261 tctatcttta tcatacatat gtacatcagc taagcaccac cctttgccac cttcattcca
    40321 agcaccttct aattgattag ctgctacagc tccaccccac aaaaaatctt taggaaactt
    40381 actcaattta atcactcctt attttattta tttaaatcat ttactattat ttaccttata
    40441 ttctatagac tttactattc ctactaaaga catagttata tttaactcgc tgaatattgt
    40501 cataagagta tcttgagcat gtgcaaataa aagtgaatgt gctctttttt ctccactagc
    40561 ttccccttgt ataatgttag tttgagctct atgtgcttct gtcataaact cttttgcttt
    40621 agctagtttt aaatcagcat ttttataatc aaacctttct aaatcattaa gagcatgttt
    40681 aatttctgtt ctagcctcac ctgcacgaag tattatttgc attgctactt ctactaattc
    40741 ttcatctatt acatgttctt cagttgtcat atttataatt tcctttcaac atctaaattt
    40801 aagcttcttg actctctaat tttagttctt ctttttcata agctttaaaa aatggatacc
    40861 atatcatgaa tagcaatact atatttattg ctaaaacaat aagccctcta acatcttgat
    40921 ttaccataaa agtagatata ccaaaaggaa gataccataa attaaacacc ttactcggaa
    40981 ttgtcacaaa tcccatactc attgataagt aagtagttat tgatattact actgttccta
    41041 tccacatagg taacattaaa aatgggttcc atgctactgg agctccaaac attactggtt
    41101 catttatgtt cattatagaa ggaacgatac aggctcttcc gattgctttt attcttccag
    41161 ctctagataa acacatcatt agcactagtg gtaatgttgc tccccaccca cctaaagtta
    41221 tccaacctgt ttgaacaact tcataagtat atatattagt tgcaagccct cctgatgcta
    41281 cagctgctgc attagctgca attgcactaa gctgaattgc ataagttatt ggggataata
    41341 cccatccact tatacctaaa gaatataaaa atactactaa gaagtttaat agtacaaatc
    41401 caggcaatgt ttgtgctata tctgatactg gtttaaatat cctaactatt atttcaaaca
    41461 tatcaaagtt aagtccataa attagtcccc aacctataaa cattattact gctataggta
    41521 acatactatc aaaccactta cttacaaaat caggaagtgc tgaatcttca ccaaaaaatg
    41581 agaaagaccc aaataaagac attattaatc cggagaataa tcctgcaact agtgctacaa
    41641 gcattccttc tcctccaaat ctattgaagt taaatatagc tccttcattc gtaatctctg
    41701 gacgaatcat cattaacatt aatccagcac ctgcaagacc agctactaca ctcatacttt
    41761 caaatttctt attcttcatt atttccatag gtaataaaaa tgctataaat agtcctaaaa
    41821 gtccaaatgt atattgattt attggactta aatcaggtaa gaaacttatg taattcttca
    41881 agacagaaac tatagttact aaagaaccta caaataccat aggcaaaact ttcattatag
    41941 ttgattgaac tgattgtacc catatattct tagttatctt atttacaaca ggagtaaact
    42001 tttcacttaa caaatctata aatttattca taaacaatct ccccatagat attttatttg
    42061 tttaatatta attctaatgc ttcttctaaa gcctttacac catctaatct tccatatgct
    42121 gttttagata tacatgctac tggaacatta tattcatcag cttttttttg tatttcatct
    42181 ttcatataat ctaaatgtgg tcctaataat aaacaatctg tttctggtaa atatttatct
    42241 atctctgact cacttctagc ttttatatct gcttctatgc cttttttctt tgcactcttt
    42301 ctcatactag ttgccataaa acctgtactt gctcctgagc cacatgccaa caacactttt
    42361 atattcttca taacaatacc tccagtattt ttattcatta atttatttct taatgaattt
    42421 ttatttgatt tgattgtatt tatattgtac ttcctaaata atttcctttc acataaatat
    42481 atttcctaat aataggaaat catttactct taaccagaca cataaatata tagtataatg
    42541 tacattataa tacgtacatt caattatata aatatcatat catacagatt ttattttaaa
    42601 actatgagag ggaatctaat gaaaacatct aaaaaagagt tatttttaaa cttcataaaa
    42661 acgcaacatg attggataga ttcatccaca ttagctaatt atcttaatgt atcaacacgt
    42721 tcgattagaa aatatgtaaa tgaaattaat tctactggtg aatttatatt atcctctaaa
    42781 aaaggatata aagtaaatct caataataac tgtcaaacca aggttgatag ttcagaaaat
    42841 atatcacctg ataacaggtt aaatttaatt ttaaaagaac ttatagttaa tagtaatgga
    42901 attaatatat ttgatttaag tgaagaattg tttgttagtc cagctactat tgaaggggat
    42961 atagtcaaag ctaataaatt tataggaagt tataatttaa agataaagca atcgaaattt
    43021 ttattgaaac taattggaaa tgagtctgca aagcgcaaat taatgagctc tataatattt
    43081 aaagaaacgg gaagtgattt tttaagctta tttgatgtac aaaaaatata tcaagagtat
    43141 aatttgacca aactgaaaga aaatataata tatatattaa aaaaatacaa tttgtttata
    43201 aatgagtacg ctataaataa catacttctt catttaatga taactataga cagaataaaa
    43261 aagaataact acatagaatc tgtcgaagta gtaaactaca tagataataa taatgatata
    43321 agcatttcaa gagatatatc aaatttttta gagtctgaat acaatttaac tttaactagt
    43381 gcagaattat actacttagt atttcagtta acaaacaaaa ccacagtctt aaattatcat
    43441 gaaatggata ctaagtcatt gccaaattat atagatgaac attttgttaa attaactaaa
    43501 aaaataatta aaaatgtata tgacttatac tttatagatt tatctgatga tgaatttgtt
    43561 gtaaaattta ctcttcatgt aaaaaattta atcagtaggg ctaaaaataa tcaagttttg
    43621 agaaatcaaa tacctcagaa gttaaaagat tcctaccctc tgatatatga tatatctgtt
    43681 tatatttgta atcaaattca aattttagaa aacatagaaa tagatgaaga tgaaatatcg
    43741 tatatatctc ttcatgtagg ttcatttttt gatagacaaa aattactaga agataaaatt
    43801 ctctgtgcat taattactcc aaattactat gatttacaat tcaaaatagt aagagacctc
    43861 gagaagagat ttaatgaatc catagaaatt attcaaattt tttcagatac tcataatctt
    43921 gattttgaca ataaggtaga catggttatt actactttac caattagtga tagatgccct
    43981 ataccttttg tttatgtaaa tccttaccta aacagaaaag attatgataa tatccaaagt
    44041 aaatttattc agattaaaga tagaaaaaat atacttactg tacaaaatca tttagaaatg
    44101 tactttagtg aatctttgtt tatgaaaaac atatatttag atagtgctaa agattacata
    44161 aaatttatgg gaaatattct gtacaaaaat aaatatgtca aacccaatta tatagatgat
    44221 gttcttataa gagaaaaaat gtcttcaact gcatttaata acaatgtagc aattcctcat
    44281 agcatgaaaa tggatgcttt aaaaactgga gtatgtttaa ttgttaacga taaaccagtt
    44341 aaatggggtg aggaaaaagt tcaaattata gctatgatac ctataaatga aaaagaaaaa
    44401 gaaaaattca attacatatt tgaaagcttt attgaaatat tatctgagtg gaataatgtt
    44461 aaagaactta ctaaggcaga taactacagt agttttatga atagaatagc ttatcttatt
    44521 caaaatatct aaatccatta atttatttcc aatatatgtt tcttaagtta ttaacacaaa
    44581 atagataagc tgtcacacta aaaagtctaa attaatatgt gacagctttt tacctaatat
    44641 actttgcttt aatactacat attttttgat tgttgtatac tagttataag attgaatatt
    44701 tgtgttgttg tagctgtcat attctcacta gcagttgctt tatccattgc ttcttctatt
    44761 gtcatagctc tatttactat agaaaaataa gcatctatac cctcttcatt acatttaact
    44821 gcatcttctg ttgtggctcc agccaaacct attactttta ccccatattt ttttgccagt
    44881 tttgcaactc ctattggagc cttgcccata gcagtttgat tatccaatct accttcacca
    44941 gttatgacaa aatctgcatc ttttaactct tcctctaatt taatttcttc taatattatt
    45001 tttattccag attctaattt tgaatttaaa aatgctaaaa aagcaaaacc taatcctcct
    45061 gctgctcctg ctccttctac attagctaca tctttactta agtaattttt aactactttg
    45121 gaaaaatttc taagcccatt atctaactcc tcaattattt ctgatgttgc acctttttga
    45181 gacccatata tgtatgcagc tccatttttc ccaaacagcg gattatttac atcacaggct
    45241 attttaaact tacattcatc taattccttt aatctatttt ctatttttat atgtcttatt
    45301 tcatttaaaa cttttccacc taatcctact aacttgttat tttcatcata aaactcaaac
    45361 cctaaggctt gaagcatacc tatcccagca tcattagtag cacttccacc tattccaact
    45421 ataaaatttc tatagccttt atttattgct tcctttatta tttccccaac accataagtt
    45481 gtagtgtata aaggatttct aagttcagtc ggaaccaatg ttaatcctga tgcttgagct
    45541 atttctatta ttgctgtact tgtatttttt aataatccat atgttgcatc cactttttta
    45601 gctattggtc cagtaacttc tatagtctct tttttaccat tcatcccttg aactaaagca
    45661 tctactgtcc cttcaccacc atcagcaagt ggcaatatct ttacttgcgc atcttcttta
    45721 accttcaata tccccttttt tattgcattt cctgcctcta ttgaagataa acttcctttc
    45781 aaagaatcta tagaaattaa tattttcata tttatctact cctttttatt tactctttat
    45841 tttaaatata taattttata agcttaattt atcttttatt ttatactcta tatttcactt
    45901 tgtatacaac ttataccctt aatattcttt tatcttataa aaaagggagt gtctcaaaat
    45961 gaacttttta gttcatgaga cactcttttt gtataaaatt aaatatatgt ttattatttc
    46021 aacaatgata aggaggatta tctctatttt aagataattc ctttttagca ctatctaatc
    46081 atttttagca ctatttaacc aatatagttt taacttggtt cattaggcac tctacattta
    46141 cagagttatt atctattgat atagattcta attctttttc atttaggtta gtccatttag
    46201 ctgttttaat atttttgtta aatactacgt tagctgattt tgtttcttct ttagaattgt
    46261 aaaatctaac tactaatgcc tcttcttttt cagccttttt tacaacactt aacacaaggt
    46321 ccttattagt ttccttaagt aaactatatg aataatctac ttctatttct gattgattca
    46381 atttcatagc attatatggt attttattat aagtttgtat ttttgttaaa tactctttag
    46441 ccatatttgc aacatttgct tctagtgtag atttttcatg agtgactatt gctaagtcta
    46501 tttttatatc tcctatcatt tgagaatctg gagtaggcaa ctttattcct gatggacgac
    46561 ctgggcgtct tgccatttct tctttaccta ggaatcctac acttctaaat aatgttatag
    46621 ctattgtgtc aaattttttc cctactattt caaactctct tgtactgttt gtaagaacag
    46681 ctactccatg ctctttgtca gacaatccaa cgaatgttag cattggatat atagaatctg
    46741 gtctttcact ccattcttcc tgttgccaaa cttccatagc atcatcatac acacttctct
    46801 ttatatgtcc aaactgatta tcagacacag aaaactttga agctatattt gttggtatat
    46861 aagttctaac cctacaatct cttgcttggt tatttatatt gaatactaca tttattatag
    46921 ctttatcatt tggaacattt ataactatat caaagtcaat aaatgaatct gctctattat
    46981 tttttctagc ttgtatatct tttggtactt ttaatctata atttatcttt atctcattat
    47041 taaatctatt tttctttata gtggaatttg ctactatatt atcgttaaat attaatttct
    47101 catttggaag tggtgaaaaa tcatactcat ctccttcatc tcctccattt tccatcaata
    47161 atacattttc aaaagtttta tttaattttt tatcagatat ctttaatgtc ccgttttcat
    47221 taacttgaat agtataaaag tcattatcta ccatgtcaca tacttcataa gctttttcta
    47281 acatatgttc aacttctttt atcatatacg ccttataccc cattgcaggt attttatctt
    47341 taaagtttat tgtatatacc ataaatgaat catagtttcc ataatgaaca atttgacggt
    47401 ctattaatcc tgcatctatt acttctttat gtaccacttc aaaatccaac ttattattat
    47461 ctttatcaaa tatttcaaat gctttcattt tagttataac ttgggcgttc actatttcat
    47521 tcctattgta aggcattaag ttaaatgcta ctaacttatc taaagccatt tcacaactca
    47581 ttgcatcaac tattcttctt ttgtaaaatg ttattaattg gtctactttt tcctcagcta
    47641 agaaaaatct attcattatt tcctggtgaa ctttatctga acaacaacat ccaatgctgt
    47701 catgagcatg atttttcatt atttctttcc aaattaactc tattaatcca tgatgatatt
    47761 caaatcctag agaatatgca atagaagcta gtggctccaa tatgttggtt atctttgatt
    47821 ctattctagc atttgctgat tttatatcca ttcttgttga gaatatactt ctatgaaccc
    47881 tcatatattt cccatctaaa aattcccctt ttattgtatc tatatcctta tttttttcta
    47941 gctctttaaa tatgttttca tatctactta agaaaaattt tctctctgga taacattctt
    48001 ttaatttata tataacttca aatatattct tttgaattgg catttggtca tgtccatttg
    48061 gaagtattat atggtcagta gttgcaccct tatcaagaac cggtaaatat ttatccattc
    48121 ttgttcttag ctcatcttca ttcattggta gatattttcc tattgcataa cctaatggaa
    48181 gtatttgaac caaaacagaa gacccatcat cactcttcca attaaactct gttttattag
    48241 ttccttttct ttcagaacat cctctccaaa acattgagta ttttatatca aacccattta
    48301 gtatttgcgg catttgagca ctttgaccaa atgaatctgg taaatatcct attttcatat
    48361 attctccaaa ttcatcacaa tcttttattc catacagtaa atttctaagt atggattctc
    48421 caccaactac catttcatca gtttgagtat accaaggacc tattattagc cttccttctt
    48481 gaactaattt ttttattctc tccttacatt caggcttaac tgctaaataa tcttctaata
    48541 tagcagtttg accatccatc acataatatg gatagttatg attattttct aacatatcca
    48601 ttatttcttc catattattt acaagtaata ttctagattc ctcagttgag aagtaccatt
    48661 ctctatccca atgcatatga ggaactatgt gtacattttt tttcattgct tatcctccaa
    48721 aacttatctg ataatatttt ctgtctaatt aaaaatccaa taaaaatact actgagttta
    48781 cacaattttt taatatttat tttatcacta taattaaacc tttgatttaa atatctaata
    48841 acgtatcttt taatgtatct ttctttgatt tttttgtttc ctagttatta ttaataatat
    48901 tgctgatatt atagctccaa gtactgccgc aaagaaccat attgatgcta taataaatcc
    48961 tgacatactt ccctctaaaa gcgccaatga gaatatcccc gctccaggca cttgaagacc
    49021 tatattgaag aatgctacta tagacccagt tatggctgaa ccagctacta atgatggtat
    49081 aacatggatt gggtcctcta tcataaatgg tattgcacct tcagttattc cagccaatcc
    49141 taatatccaa gtttgctttc ctatttcctt ttctgattct gaaaataaat ctctgcctaa
    49201 aacagtagct attgtaactc caaaagctga gaccatctta actgatgcaa atattgcata
    49261 aggtataaaa tttccacttg ctattgctcc tattgcaaat gtataggctg ctttatttat
    49321 tggtccacct aagtcaaatg aaaccatagc tcctattatt gcacctagta gtattgcatt
    49381 tattccagtc atcccattta accaatttat aagaccattg ttaagtgctg ctacaggctt
    49441 tcctattaca aataacatca ataaacctac taccaaagaa ccaactactg gatataccca
    49501 aaaacttacg aatccagcta atgtgccttt ggctggtata ttcttcttta aaaattttac
    49561 tatataacca gcaagtaaac ctcctaacat acctccaaga aatccactac ctattaaatc
    49621 agctgctaga cctgctgcaa atcctggtcc aagacctggt ttttcagcta gtgagtaaga
    49681 catatatgct gataatactg gaaccatcaa tgtacctaac atccctccag aaagctttct
    49741 taattgccat aaccaagaac cttcttgatt gtatagttct tgtaaaccaa atgcttgaga
    49801 tgctaatact gcaaatgcaa gtatcatacc tcctgccaca ataagtggta ttatatgaga
    49861 tactcctgta agtattgctg attttacatc ttttttccat gatgattttt cacaatatga
    49921 tatatcagat gatttttcta tataatcacc tttaccttgt ttttgtgcta cttctaaagc
    49981 ttcgtatatt atttcttttg ccctttttag tggagctgct actgacgttt ttatgtttgg
    50041 caaatgttca aatctttctg tatttttagg tgctacatct gttgcatata ttattgcatc
    50101 tgcctttttt attatatctg gtgtatgagg gtccaccata ccattagctc cttgcttttc
    50161 tacatacaca tccacaccta tttctttgcc agctttaatt aatgcttctg ctgacatata
    50221 tgtatgtgct atacctgcag ggcatgctgt tattgctaat acaatttttt ttgattcatt
    50281 ttgaatccta atctcttctt ttatttgttc tttcttttct tttatatcta agttattaaa
    50341 taactctttc ttagtttttg aatttaacaa catattcata taatcttcat tagacagtct
    50401 agtaactaac tcagaaagta tttgtatgtg tgtatttcca ccttcacttt ctggtactgc
    50461 tattaaaaat attagctcta ctttattatt tgagtctacg ctttcccaat tttctattgg
    50521 tgtctctaag gtcatagctg caaatgcagc ttctttaact gacttagatt ttccatgagg
    50581 aattgcaagc cctctttcaa aacctgttgg agataaagtt tctctgtaca ttacagcttc
    50641 taaatattcc tcataagatg ttaacttgcc ttcatcatat agctttcttg ctaagaactt
    50701 tattgcgtct tcctttgttt ttattttttg ttttaaaaaa acaaggtttt catttgtgat
    50761 tttcttaaat tccaaaatta cgacctcctt aaatcattta atgttatgaa tatagcataa
    50821 aataaatcat gaaaatgttt ttttgaaatt atacacacaa tttcttctta aaaaataagg
    50881 atttgtgtat ttatacacaa atctctatct taaccagtat tgttctatta ttgctctcat
    50941 aatcatcata attggaacct tatctgatat attgtaccca tttacttgtt taattgttga
    51001 atagcaatat aaacttatat ctgcaatttg ttgcaattga ttttcacata tatgtgttag
    51061 agctactacc ttacaaccct tattaactgc tgtctttgct gcttctataa cttctttcgt
    51121 ttctccactt aaactaagcg cgaaaaacat atcttgttca cctaattttc ctatatcatg
    51181 aagtacttca tgtctttggc tatagtaagt tatattatca tgcccacctc taatttcttt
    51241 tactaatatc tcacataaag gaatgttttg acctattcca taaactataa tactcctaga
    51301 tttttgaatt atatctgcta ctttatttat tttttcataa tctattagct ctaatgtctt
    51361 ttttatatct gattcaaaac cattttttgg ctctttatcc tcatttttta actcttcttt
    51421 taaattgttt tttagttgag agaaaccatc atactccatt tttttacata gcctaactat
    51481 ggtatttggg acagtaaaaa atttatctgc taaagattgt attgataatt ttactatttc
    51541 ttctttgttt tttctcatat actcaattat catgtcttct gtatctgtaa atttatactc
    51601 atattttttt gctctttcat aaaattccat agtttctcct ccatctattt tattattcat
    51661 aagtaaaatt caaaaatatt agctaaaatt ttcgttaata taattataat ttaatatttt
    51721 taatttatta atgcatacta ctctttttat tatacataaa aaatgtgtac ttcctatatt
    51781 gatagagatg attcaggttt ttattttgat acatctctat caatattttt ttagaaaata
    51841 cacatttttt tcaaatactt atttttaatt aaaactttag gtaatctatt ctttatatat
    51901 aattattaga cttgaaaata tctttaaaat caaccaattc aatattattt ctttttaacc
    51961 acattttaac ttccttatca catagcattg catgttcata tactctctct tgaataagag
    52021 aagatgattt tataagacta taatctataa atcctggatg agtaaccatc aatgttatat
    52081 ctttatctaa atttatatat ttttcttcaa ataatttaac aaaagattta tttaacttgt
    52141 aaaaatcatt ctgaggatat ttacttggca tgctaataca tgaatctaaa taatagggac
    52201 aacttggtat accgtattct tttgcagcat cacacattgc tgatattgat acatcttctt
    52261 ctataccatg tatatcaata tactcaggca atttactcat atacaacttg taagtttcaa
    52321 tttgagcaca aacttcttca tatacttctt cataattaaa aacctcttta cctgtcagta
    52381 gttgcgttct tcttattttg ctagaaacaa attttccacc ttcatctaac aaattcttta
    52441 tcttaatatg ttctttacaa caaggttctc ctattactaa attaacatgt aaaccaaaac
    52501 atacatcatc attttttacc atttctatag cttgtcttga aaaaggcata ttcaccatga
    52561 ttccagcact tgtaacaata ccgttcttat aagaagatat tatccctagt gtaacacctt
    52621 ctgaatatcc tatatcatct gccctaacta ctaatttctt cattttatgc atctgcctgc
    52681 tttctcaact catcaagttg catttccatc tcatccttta ctttaacaac atccgttcct
    52741 atgatgattt gaactgatgt attactcaat ttaattaccc ctttggctcc tgttttttta
    52801 atattatcat cattcaaaat agtggcatcc ttcacttcta aacgtaacct cgtcatacaa
    52861 ttattaagag tcactatatt ttcataacca ccacaatttt taattatctg cttagccata
    52921 tatgaaaaat taccatgaga aagttttaaa ttcttttctt tttcactaac tttatcacct
    52981 atctctatct caacttcatg tcctggtgtt tgtacattat cttttattat tatatattta
    53041 aatgttacat aatataatac aaagaataca actcctacag gtataactaa ccacgcttta
    53101 tctccataac taaaacttaa gacatagtca attatacttg ctccattaaa tgaacccaag
    53161 cgagcaccca gtaaatatag tacaactcca cctagaccag aataaaaaga atgaattata
    53221 tataactttg gagatgcaaa taaaaacgaa aattctattg gttcagttac tgttgccata
    53281 aaagaagtca atgctccagc ggtcattgca ccttttactt cttgcttctt tttatcttgt
    53341 gctgttttat atatagcata agaaatagca ggtattgaaa acatcataat tatgtaaaat
    53401 cctgtccaga agaatcctgc atttgggtct cctgccataa atctaggaat ttctcctctt
    53461 ataacttctc ctgttgatgt tgtatattca cccatttcaa ataatatgta agcatttaaa
    53521 acttgatgta acccagttgg tattagtgca cggttacaga ataaaaacaa gaatattcct
    53581 attgcaccag ctcctcccaa ccaaaaacca aattgattca ttgcagcttg tggatatatc
    53641 cataataaac taaatatata tgataaaatt aaagcaacca ctggtgatag tgtaattaca
    53701 catttttctc ctgcaaagaa gcttaaaaat gctggaactt taactttata tgttttattg
    53761 taagtccatg ctatacaagc tccaacaatt atcccaccaa atacacctgc atttaatgta
    53821 tcattcaata actctaaatt tgacttaaat atcatatatg caactacggc tgcaaccatt
    53881 acacttgtct tgtctttaga ctttgaataa gcactaactg ctcctaataa aaataagtag
    53941 tccacatttg aaaatactat attagatata ttagatatag ctggcacatt aagcatattt
    54001 ggaccaaata ataatcctaa tatacctgct gctggcatta cagctattgg caccagcata
    54061 gtctttccta aaagtgacag ttgttgcatc attttgttta agaacctctt catatccaat
    54121 cccctttcta tttcggttta attttttgtg aaaacgattt attcacattt tatattttaa
    54181 atattatatt tgtttaaaca aattgtcaat attttttatt cattttattt ttatcattta
    54241 gtttcatatc taaatccttt taagtatatt gctatatcta gcctattttt taaatttatt
    54301 ttttttattt ttaaaatatg tttaaccatg tatttgaaac aacccacaaa atagttcaaa
    54361 tttatccttt ttatggttta tataattcta ttttcactat acatgtctaa acacatttga
    54421 tataatttgt tcatatcagt tcaataatat tactaaagac ctttaaggag ggataggggt
    54481 atatggctaa aaataaattt tcagagatat atgaagtatt gaaagaggaa atacttgatg
    54541 gtaaatatac aagtaatatg atgctgccta ctgaacttca acttattgaa aggttttcat
    54601 gtagcagaaa cactgtacgt agagcaattt ctcaattaaa cacagaagga tatgttcaaa
    54661 gtataaaagg caagggtgtt gtagtgttag aaaatagttg ttctaatgat ttttttctaa
    54721 atatgcacaa tttcaaaggt gtagaatcga ttgtagaaga taaaaaggtt aatactgcta
    54781 ctagtgttct ccacttcagc aaaatcttaa ttgataataa gttatctaaa aagacaggtt
    54841 ttaaagttgg tagtgaagta tattaccttc acagactaag atatattgat aacataccta
    54901 aaattctcga cattaactat tttctatgta gtatagttaa agaccttgat gtttctatag
    54961 cacaaggctc tatttataaa tatatagaag agtgtatagg tacaaaaatt gtttcatcca
    55021 gaaagatatt taaaattgaa aaagctacag agcttgagtt aaaaacgcta cctctaaatg
    55081 attataattg tgttggtgtt attaaaaata gtgtatatac tgatgatgga aagctctttg
    55141 aatatacaga atcaaagcat acacctgaaa catttgtatt catggatgtt actcaaagat
    55201 attaaaaaaa ttatgtttaa ttaaacataa tttttttacg tttagacact ttatcccata
    55261 ctaacctcac tattctatat acaattatat gtttatacaa ataattattt attgtatatt
    55321 ttagctttaa tattagttta atacttgaca atttgtttaa acatattata attaacttat
    55381 aaattacagg aggtatcaat tatgaaaaat tggtggaaaa aagcaactgt ctatcaaata
    55441 tatccaaaaa gtttcaaaga tagcaacaat gatggtatag gtgatataaa tggtattata
    55501 gaaaaattag attatctgta ttctttaggt gttgatttat tatggttgac tcctatgtac
    55561 gtttctcctc aaagagataa tggatatgat atagaagatt attataatat agacccaaaa
    55621 tatgggacaa tgagtgactt tgaaaaactc ttaaaagaag ctcataaaag agacataaaa
    55681 ataatgatgg atatggtcct aaaccataca tctactgaac ataaatggtt taaagaatct
    55741 aaaaaaagca aagacaatcc atatcgtgat tattatttct ggaaagatgc taaacctgat
    55801 ggttctgttc ctaataactg gatatccaga tttagtggaa ctgcttggaa atatgatgaa
    55861 actactaacc aatattattt acatttattt gaagaaactc aagctgattt aaatttggaa
    55921 aatgaaaagg ttcgtgaaga atgttacaaa gtactagaat tttgggctga taaaggtatt
    55981 gatggttttc gtttagatgt agtcaaccta ctctccaaaa cacctggatt acctgatgac
    56041 cctataacag ggccaaaagg agatggaaga actcattacg ctgatggtcc tcgtatacac
    56101 gaatatttac ataatatgaa tcaaaaagtt ttcaaaccta aaaatatagt tactgtaggt
    56161 gaaatgtcat ctactacacc tgaagagtgt atcaactata ctagagaaaa tcgagaagaa
    56221 ctatctatgg tttttagctt ccatcatatg aaaacagatt ataaaggagg cgcaaaatgg
    56281 actaatgaaa tgttctctct ggataaatta aaaaaagcac aatctgactt tcaatataaa
    56341 atgtatgaag gaaagggatg gaatgctcta ttttactcta atcacgacca acctagagct
    56401 ttatctcgat ttggagatga tagatttttt cgtcaagaaa gtgctaagat gttagccata
    56461 acactatttg gtctacaagg tactacatac atatatcaag gtgaagaaat aggtatgaca
    56521 aatgcctact ttactaaaat agatgaatat gatgatgctg aaagtaagaa tgcatactat
    56581 catatgatta aatctggtat tgatgaaaag gaggcattgc ttatacttca acaaaaatcc
    56641 agagataatg cccgtactcc tatgcaatgg gataatacta tttataaggg gttttctaat
    56701 cataaaccgt ggttaaaagt taataatgac aatattagtg tagaaaatga gcttaatgat
    56761 tctcgttcag tattttatac ttatcaaaga ttaatcaaat atcgtaaaca atatgatatt
    56821 tttacagaag gtacttatag attgttagat gaaaatcata aaaatctatt tgtttatgaa
    56881 agagagtata aaaaccaaaa tcttcttgtt ataagtaatt ttactcatga taatgtagta
    56941 tataaattac ctgaaactta tttgaataaa ttaaactaca caatacttga aaccaattac
    57001 actagagata taattgaaaa taaaatggaa ataaaacctt acgaaagtat aatgatatat
    57061 tataaataaa gttatttata taaaaattgg tataacacca gggtgttata ccaattttac
    57121 taaattcaaa ataacattta tagaatatct atagattaca ataattattt gacttctaca
    57181 tcattatatt tctttgaata tactttagtc atatagaaat atataggaac tacaattatc
    57241 atcccaccta atccccaaac tatcttcata ggttcatcaa tacctgcttt tatcagtaat
    57301 aatatactta taataactgc aaatattggt ataattgctc ctccaggtat tctaaaattt
    57361 acatctttat cagcatactt ctttctcaat attagtacag ctaaacatgt aggaatatat
    57421 tgaataaatc tagatacaac acttatttga gataatgttg tgaatgaacc cgaccatgct
    57481 attagtaaag ttcctatgac agatactata attgctatat aaggagctcc atttttatta
    57541 gtttttctta tagacttagg cattaatcct tgttcaacca aagcagcagc agaccttggt
    57601 gtaaatatgg atgatgcaat atttatacct ccaacagata ctatagttcc tattgtaatt
    57661 atatacccac ctataggacc tagcatctta atggcagcat ctgcaacagg agtagctgac
    57721 cctgccaagc cattacccaa cacccctata cttattccca gtataagcat atatattact
    57781 gatactacaa acattaccat taccaatgct ttaggtacat ttttcttagg gttctccata
    57841 tcttttgctg caacagctaa agattcaaat cctgtaaaag cataaaataa agttatagat
    57901 actgctacaa aagctgatga cgcactttga cccttttgta ttattacaaa tggggtaaaa
    57961 ttagaaccct ttataaagaa taatcctaca gctataaata aaactattgg taataatttt
    58021 cctatagtca tgatattatt taagtgctta gattgtttca ttcccataaa atttataatc
    58081 ccaagtccta caactaatat accaattatt atatttttaa taagtggttc tgctgcttgt
    58141 ggccatacag atgatagtgt tacagcaaaa aaatttgcca tagtagccca agcaatcata
    58201 ctaactatcc acttcataaa tccaacttca aatccaacaa aatctccaaa tgcctctttt
    58261 gcataaagat aaggccctcc atcagtatca aaccaactac ctgcttcagc aaaacataaa
    58321 gcaatagaaa cagctaaaag catacaaaaa actaatacta atatacttgc tggacctata
    58381 tctgtataag catcaccagg taacccgaat atacctgacc ctattattgc attgattcct
    58441 aatagtacaa tactccaaaa acctaactta atgccttttt ccattgttat tccccccaaa
    58501 gccaacttaa tatatttata ttttttattt agatgcttct ttaactattg ccataaatat
    58561 ttttttcata ttatcataat ctctagtcat catttctgga tgccattgta tccctattac
    58621 aaactcactt ccttcctttt ctatagcttc aattaaaccg tctttagatg tagctgaaac
    58681 aatatatcct ggtgctactt tgttgacaac taagtgatga aaactattaa ctaaaacttc
    58741 tttttctcct aatatttcat ataattttgt cccctccttt atctgtactg tatgtgtagg
    58801 tacattagat agatgttgct gattgtgttt aatataagca ccttcaatta aagataaatc
    58861 ttgatacaaa ctacctcctt cagttacatt tataatttgt tctcctctac atatacctaa
    58921 aactggtttt ttcatatcta aagcatgttt taataatttc atatcaaaac tatctctctt
    58981 tggtgatatt gcccccaact tattatgagg ttcttctttc catatgagtg gatttacatc
    59041 ttgtccccca gatagtataa gcgcatctat attagatacc tgctccttta taatctcatc
    59101 atcatataca atcggtacaa tatatggtat agctttacac ataactactg attgtatata
    59161 atcattattt acataagccc tttcatagcc aggaaacatt cctccttcat caattagtaa
    59221 attacctgat attcctatta ttggatattt ctttttaacc aatttcttca ccccttaact
    59281 tatttgtgaa caataaaaaa tgctacttct cttaaagagc aagtagcata ccaagatttt
    59341 atttatttga acattataat tcattttaga aatacttttt ttgaatacaa gtaatataaa
    59401 taactttatt tacttatatt ttattttaat cctgtttagt ttgacttttt taaattgttt
    59461 taagtacaac taataggtaa aataggcatt tagattttcc tttttatcaa cctattaaat
    59521 actgtttaat atattttttc cccttctatt taattcactt ccctttctac cagattcaca
    59581 tattatcaaa tccagctctt ttaacatatt aagtattccc cttacctcac cttctgttat
    59641 actgtagcct aattcattta ttgaattaac aataccattt ctacctatat ttttattaag
    59701 attattggca ttttttaaac actccattac taactttact ttctctatag atgttctatc
    59761 ctttaatatg cttatttctt tactaaaatc ttcagttata tttaataagt tatgaggtaa
    59821 atcatttatt aaaataacat cctcagaact cataatgtta atatacatag cagtattttt
    59881 taattctctt atattacctg gccattcata tgcattaata atgtcttgaa caccttgact
    59941 aattgctctc ttccttttca taaaatgctt taacataact tctatatctt cttttcgctc
    60001 tcttaaaggt ggtatattta tgggcaatac atttatccta tagtataaat ctgctctaaa
    60061 tctatctcct tttatcattt ctaataaatt tttatttgta gccgcaatta ctctaacatc
    60121 tatatctatt acattttctc ctccaaccct cattacttga ttctcttgta atactctaag
    60181 taatttgctt tgtaagtata taggcatgtc tcctatttca tccaaaaata tagttccatt
    60241 gttagcctgt tcaaataatc cttttttacc tccttttaaa gctcctgtaa aagcaccttc
    60301 ttcatatcca aaaagttcac tttcaagtaa attttctggc atagctgcac agtttatcgc
    60361 tataaatgac tgactatgtc ttggtgatgc attatgaata gactgagcca taagttcttt
    60421 tcctgttcca ctttctcccg ttataagaat tgtataatca gattcagcta ttttcatacc
    60481 taatttctta gttctttcca tcttcgttga aactgttttt atatcatcaa aagtatatct
    60541 agcaacttgt cccttttctc taagcttctt tgataggttt tgctccaatt ttcttatata
    60601 agttatttct tgaatacaaa aataataccc agtaaccctt ccaaaactct ctatattata
    60661 cttatttacg tttaagtact tattattaaa ttttataact tcatcatgtg ccttactcaa
    60721 actaaaaatc tttttaatat tctcattctc taattcatct atatttttcc ctataatatt
    60781 actttgaata tctagtatat cccttaaact attattgcaa ataattatgt ttcccttatc
    60841 tgataccatt gccataccta agtttgataa atttattata gaatttaatt catctgcttt
    60901 tatagtaagt tctttgtatt tagtatttat tcctgaatat aaactaatta tttcatctgt
    60961 atacttaatt agcctctcag taatttcttt atcatccagt ttgagttttg ctattatttg
    61021 aataaacgtt gaactatcaa tatacctatg gcctaaatca ataacttctt ctatatattt
    61081 tggaactaaa cttgactctc ctggcgttat agctatctta atatttctat attcttcatt
    61141 gggattgtat ggtataaaat tcaaatggtt tattcctata ttgtataaat tagatattgt
    61201 ttccaataca gtatgcttat tatcgtttac tactaataca tctatttctt ctggcaatga
    61261 aaataactta tatatatcct tttgctttat gctcctattt atagttatta tctttgagac
    61321 atcttctaca tattttttta tttttaatac tctatcatct atcattacca atattatgtc
    61381 atcttttatt aattccttct ctgttatttt atttaaataa taatttttta tcattacttt
    61441 atctttaaat atcatttcta gatttttatt taagaaacat tctaatttag acaacccatc
    61501 agttactact gctattgttt tcatttgatt actcctttta aaatatactc tactctttta
    61561 taatatacat ctgattttca ctttaatagc acaaaaaatt gtacatttag tatactaaca
    61621 tattccttta ttagttttat gtccctaaat atcatagttg caatatctat catatttctt
    61681 aaacttctat aaagcaaaaa ttgtgccaaa ttaataattt tcttaatttt attctaaagt
    61741 tcctcaatag tatttacaga cttagcactt atagcttata agtctttata ttattaatct
    61801 atttttatag gttaattgta caaatcatat atcactttta cctattaaat gcattataaa
    61861 aaagatggaa taacgattcg tcactccacc ttttttgtaa taaaaaattg tatatataaa
    61921 cactatttac tactcttcta agtacaaaca gtatctacat actataacct ttcaccatta
    61981 gattctatta cttgtttata ccagtcaaaa ctctttttct taatcctaga caagtctcca
    62041 cttccatcat catacttatc cacgtatata aacccatatc tcttggtcat ctcaccagta
    62101 gatgcactta ctaaatctat acatccccat ggagtatatc caaataaatc aacaccatct
    62161 tctacagctt ccttcatttg ctctatatgt gcttttaaat actctatacg atagtcatca
    62221 tttatcactc cattagattc ttttttatca taagctccta aaccattttc aactaccatc
    62281 ataggtattc tatatctatc ataaatttca tttagtgcat atctaagccc ttcagggtct
    62341 atttgccatc cccattcact agacttaaga tatggatttt tcactccacc taatatgttg
    62401 ccacttgtat tatcttcttt agaatcacta cttacacatg tagacatata ataactaaat
    62461 gtataaaaat ctacacaacc ttcttttaaa atttctaaat ctcctttttc catttttatt
    62521 tcaatattat tttctccaaa atatctattc atataagttg gatattcgcc acgcacttgt
    62581 acatctccac agaaataatt cattactcta ctatctcttt gagttttaac tgcatcatct
    62641 ggattacaag tataaggata cattgttgca aatatttgca tacatccaac ttgataattt
    62701 gaatctattt catgagctaa tttaacagcc ttagcacttg ctacaaattg atgatgtaac
    62761 ccttgaaaac gaagttgagg tatatctact tggtttataa aatctgtagt cccttcattt
    62821 aatattcctt gtgataagta gcctcccata ggcattgtag ctgaatttat ttcattaaat
    62881 gtcagccaat attttacttt tcctttatat cttttaaata ttgtttcaca gtaattcata
    62941 tacaagtcta ttacctctct tgaaacccat ccattgtatt tctctgttat tgcaaatggt
    63001 atttcatagt gagatattgt tattaatggt tctatattat attttaaaca ttcatctatc
    63061 acattttcat aaaatttaag tcctgcttca tttggttctt tctccattcc agttggaaat
    63121 attctagtcc atgctattga aaatctaaat accttaaatc ccatttctgc aaataaagct
    63181 atatcttctt tgtatctgtg ataaaaatct attgcttcat gacttggata gaatgttcct
    63241 ttttctataa ctctcgttat tcttttactt actttttgag aacccccagt acacatatct
    63301 gaacaagaag gacctttccc atctaaattc catgctcctt cacattgatt tgctgctgtt
    63361 gctccacccc ataaaaaatc ttttctaagt accattactc tacctccaat aatttattta
    63421 tagtaaattt attttaaaat aaaaatacat atatctaatt tttaattaat aaaactctct
    63481 tttaagctaa aacagttatc aattcctgac tattatttac atgcttttgt gtattttcta
    63541 taatatccac ataatcagaa taattagtaa caactactgg tgtttctaag caatatcctt
    63601 cttttgatat gctttctata tcaaaagtta ttaatgtctg acctttttta atcttatccc
    63661 cttgattaac aaaagatttg aaatgtttac cttccaactg aatagtattt aaccctatat
    63721 gtatcaatag ttcacaacca ttatcagata taattcctat tgcatgtttt gttggaaata
    63781 aagtcattat agtcccatca aatggtgcca ctacttttcc ttctttaggt gttattgcta
    63841 caccttttcc caatatacct gttgaaaacg ctgcatcttg tatctgctcc aaagccttta
    63901 cttcaccttt tattgggcta tatactactt ctcttttgtt tttttctaca acgccttgcc
    63961 cattattatc tttagaaatt tctactatag aatcatcttt ccaaaaaaat aatgttaaaa
    64021 caaatcctac tacaatagct actattattg ctattatagt tgcataaaca cctttcatat
    64081 ctccagttgt tggatttata taattaggta taccaaatat tccaagtcca cccattacat
    64141 aactttttac attcattatc cccattacag ctcctgccac agcaccacct acacaactat
    64201 atatgaatgg tgtttttctt ggtaaagtta ttccatatat tgcaggctct attacaccac
    64261 atatccctga aataactgca ggtatacaca gctcttttat cttcttatct tttaatttaa
    64321 agtacattgc tgctactact gatgtttgtg caaaagatgg tacaaatgct cccactaata
    64381 ttgtgtcaaa ccccatagtc ataaggtttg acatagctat tggtactaat ccccaatgta
    64441 gaccaaatat tacaagcact tgccacaata aaccagttaa tacaccacat aaaattggtg
    64501 aaaagttaaa taaagctaaa aatccatttc caactatatt agaagcatag gtagctatag
    64561 gccctataac cataaatccc aatggtaaag aaactaataa aactgccatt ggtacaaaga
    64621 aattctttac tacatctggt attattttag caaacacttt ctccaactta gctgctaata
    64681 atactattat aattactggt actactgttg aagtgtagtt cattgctatt actggtaatc
    64741 ctaagaaagt tatatatact ggtgattcaa atattgttcc agtaaataga ttgtataaag
    64801 gttgacctat agatgctaga gtatctaatt gaatacctga atagcataaa actgcaccta
    64861 taagcattcc aataaatggt tttaatttga atttcttagc agctgtatat ccaagaatta
    64921 ttggcatata ctggaacatt gcatctccga ttgcatttaa cataatatat gcaccatcag
    64981 tttctccata caaacccaat gctgtaaata ctgcattaaa tccttttatc ataccagcag
    65041 cacacagaac tcctaatatt ggttgaaaaa ctcctgatat tatgtctata aacttattaa
    65101 gtaatccttt tggcgcatct gaatctgaat tatctattgt atctcctcct aatccagcca
    65161 aatcgcacac aacttcataa acatctggaa catgattacc aataactact tggtattgac
    65221 ctgcactttt cattacagta actataccat ccattttttt tagcacatca tcatttgcta
    65281 ttgattcatc ttttaattta aaacgtagcc tcgttataca gtgagataaa ctgctaatat
    65341 tatccttccc tcctacattc tcaatgattt cttttgatag ttgttcatat cttctcattt
    65401 tttctcctcc attttctttt tgaaatatga aggtttggcc aacttaatgt caccacccaa
    65461 tatatataaa cctaattatt aggatttata tctgacttaa tacttttact taccactctt
    65521 tcaacgtgaa tagttaagta taatttttcc tcatctgtta aatcgtagtt gtatttttta
    65581 tgtataaatt catccaactt ttctatgcat ttataagcat ttttatattt aacttttatt
    65641 acatttagta aatcatcttc attattttca tgtaaattat tactaacaat tcgttctgca
    65701 aaatacttta agtgagtaat aaatctatga taataaactg aatcttcatc aaatactatt
    65761 ctgtaatggt atttaaccat atttgaaatt tcttgtatta tttgtgtaat ctcatatata
    65821 gtttttatac tttgatccat ttgtgcatta actatatgca gtgctataaa tccagcttca
    65881 tcttctgata gtctaatttc aaattttttt tctatataat ccaatgcttc taaccctatt
    65941 ttaaattcac tcttataaaa tctttttata tcccaaagta atacattttt tacacttact
    66001 ccttcacgag ctctttctat tgcagtatac atatggtctg ttaaagaaat ataaatacta
    66061 tcatttaatc ttttccctag tttttcttta gcatataaaa ttatttcatc tgataattcc
    66121 atatatctta taggaatatc tgctattagt tctttaaatt tatcagatat atttgggtct
    66181 gaaagtttga atatcttatc aattttatcc tttgatatat gctctcctgt acgtttttta
    66241 taagctaaac ctctccccat aactactatt tccttatgat tttcatcagt tgtaataatt
    66301 acattattat tgagtatttt ttcaataatc attgtgatgc cccctttatt agcattaagt
    66361 taaaataaat cttttttaat tatttagaat atttaaaatg taatttatta cataaaaaaa
    66421 aacctattta ttacacatta aatagactcc catgagtcct gagtaataaa taggtttagc
    66481 ttgtatttaa acaatcacta tccgtttgta ttttatttta attattattt attatatgtt
    66541 agcaaattta ttttttagtg tcaacttttt ctcaatattt taattctaaa taaattatat
    66601 aaattgcatc tgcaaagtac tatttttata aataaacaaa ataaaaaagg tattacttgc
    66661 tttaagtaat acccatttat ttttaattta agtggttttt cttaatctca tcaagtagtc
    66721 catcttctaa ataaatttct aatgcacatc ctaccattgc ctttactgct ttgtgaagtt
    66781 tatcataagc ttcctcagaa tttgcataat ttagaaattc tttttcatga acatatacca
    66841 ttggacttat atctaaggct atttctgtat acatagtagg gcaaacttga cttacattac
    66901 ctatatctgt agaacctact ggaccatctt taccttctaa tatatctgtt atacctgact
    66961 caatcaaatt attctttgtt atcttttgaa gtactttgtt atttattatg ttgtcaaaag
    67021 aattttcaaa gtatctataa gaaagcttag ctcctgtcat taactctgca ccttttgcac
    67081 agtttataac ttttttagtc acttcatcta aataacttct ttcagcagct cttacaaaaa
    67141 acttacaagc tgctttttct ggaactatat ttggagcctc tcctccattt gaaactattc
    67201 catgtattct aacatctgat tttacatgtt gtcttagtgc atttatccca gcaaaagtaa
    67261 gttgtactgc atctagtgca ttaactccca tatgagggtg actagctgca tgtgctgata
    67321 ccccttcaaa actaaactct aatgaatcta ttgccaaagc cttacagttt atattattaa
    67381 aagcttctat atgcatttga taacttacat caacatcatc aaaagctcct gccttaacta
    67441 aatcacattt tcctcctgtt gtttcttcag ctggtgttcc tattagtaca atttttcctt
    67501 taaaattttc ttttaactta cttaatacta tacaagctcc tagtgtagat gctgcaatcc
    67561 agttatgccc acaagcatga gctggttctt tattgtctcc atatccagga agtgcatcat
    67621 actcagctaa aaatgctatg gtaggtccat cattatctcc taattcagct ctaaaagcag
    67681 tctccatatt acaatatgga taaactgtat caaatccata ttccttcatt ttctctacta
    67741 agtattttga tgatatgtat tcttcttccc ctaattctgg attgttaaat ataaaattac
    67801 aaacttcttc aatttctttt ccaagttcat tctctatctc ataagctctc ttctttatac
    67861 tctctatcat aacttacctc ccactagaat cctagatata atcctattgt caaaaatacc
    67921 attacaagaa ttgttaatac taccattaaa ggaattacga atctaaccca ctttgaccaa
    67981 ggaatattgg ctactgctag tgctcccatt aaaacacctg atgtaggtgt tattaagtta
    68041 gttacaccta taccaaattg ataagctgta actatcattt gtcttgatac atctactaag
    68101 tcgccaagtg gtgcaagtac aggtattgta agtgatgcaa gccctgatgc tgatggtatt
    68161 aaaaatgcta ttaatccttc tattaagaaa gttaaattta taaatatagt ttttggtaat
    68221 ccatttaata aatttgctgc agaatttaaa attgtatcta ttataaatcc attttctgct
    68281 attattacga ttccacgtgc aaatgctata cataaccctg ctgtaattaa atctcctgct
    68341 ccacttataa atgaattaac tatctcatct tgtttaagcc ctgatgctat tcctgatatt
    68401 atacctatag ctgtaaatat cattgcaatt tcagttatgt accactctag tcttaaaact
    68461 ccatatatca taatcgccat acctattgtg aaaatggcta ttaccatggc ttctcttttt
    68521 gtaaattcct ttatatttgt atcactatta accattgatt cttgatttag tgatatatcg
    68581 tgtactaaag attttttagg gtctttttta actttattgg catacatcat aacaaatcct
    68641 atagatatag ccatatatat tataaacata acaactctaa actctattcc agaacctggt
    68701 actatttgtg ataatgcttg tgctattcct acagagaaag gattcgtagt agctgctcca
    68761 aatccagctg ttgctcctat aaatactgtg gccactgctg tcaatgaatc atatcccata
    68821 ctggtcataa gtggaataaa tatcatataa aaaggtagtg tctcttcact cattccaaac
    68881 gtcgtcccac ctagtccaaa tagaatcatt gaaatcggta ttattaaaaa ttgaaacttc
    68941 ttcattttat ttacagtatg agctataaca gcttctatag cccctgtttt attaactatc
    69001 ccaaaagctc ctccaactat taaaacaaat ccaactactt cagctgcatc tataaatccg
    69061 tttataggtg ctgtaaatac atcagtaatt ccttgtggat ttgatggtgc tttttcataa
    69121 gttccagcta caacaactga ccttccgtct atatctactc tttcaaaatt tccacttggc
    69181 actatccaag taagaattgc cattaacact attagcgaaa atattattgt aagtgtctgt
    69241 ggcatagtaa actttttctt ttttaaactg ctcattttta atctccccac tttttaaatt
    69301 aaatatttta ttattgcatc cctttatcct attttgtata gatgtaaaaa agctcaacat
    69361 ggatacatgt tgagctaaat gtgctaaata caacatacct gcatcttccc tttatcttgt
    69421 ttaagatagc tctccatcaa aattcgggat aattttgatg acagtactgt acctattaaa
    69481 tacagtccca gcactagctt tggacactag cacttcggca cttactcctt ttatattgcc
    69541 tcattggttt ccaacctctt caatattaat cttagtaaat gcaacctcta cctcaatctg
    69601 ttcgttagat gaggtaattc ttattaattt ttattgattc aaagtaattg tagcataata
    69661 tgtcgagatt gtaaatattt tcagtataat aattcttact ttatgatgtc attccatttt
    69721 tcacaaataa ttttttctcc atataattta aatactgtag tatattttaa atacacacaa
    69781 actttccaca aatttttaat tattataata cctttataat aaaaaataaa agccatgtaa
    69841 acttatcaat ttacacagct ttatatacat taatctactc caagtacagc tttaagcttt
    69901 gatgctattg taataactcg tggaccaaat ataacttgaa ctgagttttt ctttttaaca
    69961 actccacttg cacccaattc atttacccag aaaccatctt catagaatcc atcaattgaa
    70021 tttagctcta ccctaagtct agttgcacag tttgtaacat tttttatatt attacttcct
    70081 cctaaacctt ctattataga atgtacaagc tttgtatctt tatcatctac ttcctcttct
    70141 actgcattgt tgtatgctgt tttaccagca tctacagatt gtctttctct atattctttc
    70201 ttactataaa gcttaacttc ttctgaactt cttcctggtg tttttatatc aaacttctta
    70261 attgcccatg taaatattat ataagtaact ataaaaaata ttggtatcaa aaataataat
    70321 ggtaacgcat ccactttttg tggttgtaat aaattaggaa acatatttct aaatgattct
    70381 cccattatag aaacattaaa catttctgta aatacatacg tcaatcctgc cagtggtaca
    70441 tatgctacaa aatacaataa tggtgcaaca aataggaatg tatactctat tggttccgtt
    70501 acccctacca acatggctgt tactattgct ggtatcaata tacttattgc ttttttctta
    70561 ttagcattat cagccaactt atacatagct aaagctgctg ctggtaatat tgcaaaatgt
    70621 attaataaac gacctgttgt gaagttacga gttatataag atagtgtatc tgggtctccc
    70681 atttgagcta atcttatatt attaacaccc tcgtaaattg ctccacctac ttccattgtt
    70741 ccacctactc ttgtatattc tatagggaat gcaattaagt gatgtatacc aaatggtaat
    70801 aatgctttat ccattgctcc aaaagtaaat gttcctaata atccactaga acctatccaa
    70861 ttaccaagtt tagataatgc caaccctact ggtggccata taaagtacat tgctgtaccc
    70921 aatactattg atgctataaa catcataatc attgaaaatc ttggacctga gaaaaaagat
    70981 aatgcactag gaagttctat gtcaacaaat ttgttatgta ctgcactagc tacaaatcca
    71041 gctattatag ctccaaatat actacaatca tatgaaaaga ttcctaagaa agaaccatat
    71101 aaagaacttc gactatatgc ttctgcctct cctaatccag atgccataaa cgcttctgct
    71161 gtaactgtat ctgaatttat accatttgat gctaacaatg tacttataac tgtgtgcatt
    71221 cctataaata aaacaattcc tcctaatgca ccccatcctt tttccttttt agataatcca
    71281 aaagctatac ctgtaacaaa gaatattggt aagtatctca ttaccataaa tcctaaatcg
    71341 tttattactt taaagaatac atttataaat gacccttctt ttattagtcc gctaagccct
    71401 aatgcagaaa cgttttccat atttgcaaaa gctgacccta atcctataaa tactcctgct
    71461 acaacaagta atataacagg tatcatcatt gctccagaaa gtacttgtaa tttatccttc
    71521 atagtattat ttcctccatt tttattgaaa tgccaggcta tataatgcat atatatatac
    71581 ttctattgct ttgtataatg tatctatact tatattttca tttgcctgat gtgaatttgg
    71641 attatctccc tctattgtgg gtccaaatgc aactccattt tttaacactc ttgcataaga
    71701 tgcagctcct tttgtaaaaa gttcggcttc ttttccaaaa ccttgtttat atgcacttga
    71761 taatttattt attaattcac tatctggttt tacatacagt aattttaaat ccttatatat
    71821 atcaactatt aaattttctt ttttcccaat ttcattaatt ctattagtta taaactccca
    71881 acttataccc ttaggatatc taaagtctat tttcatattc aattcatttt tctctaagaa
    71941 gatactcatt atacatattg tagaaactcc catatctaca tcttctttat atatccctaa
    72001 tttttttcca tgattatcat ctgtaaagta tgaatttaaa atatccctta ttataacgcc
    72061 tttatcattt agctttttaa ttttctctaa atcttttgct aaactaaatg cacagttata
    72121 tgacctgtgt ggatttctcg ataaagccct ttctcctgta tatcttacta atactttacc
    72181 ttcttctagt tcttctatct ctgtataata agctaatgat tctactaaat ctttcttatc
    72241 atcagtttta aaaacagcct caattttgtc acatacaaat ccatcttctt tgtttgactt
    72301 tatatcaact aaattgtgat ttctaaattt atctttatct attttctttc ttagattgaa
    72361 gtataaaata ccattttctc catttactat tgggaagttt ccatctggag aaaatgcaaa
    72421 ttttggctgt tcattgacac taaagtaatg ctccatacat tcccaagtcg tctcttcagc
    72481 tccacccact atcaatctaa tctttctctt cggcttttca ttaagttctc ttaagaaaag
    72541 taatgcatat aaaatcccta tcaaaggtcc tttgttatca ttaactcctc taccataaag
    72601 atagttatct ttttgacata ctttaaaagg tttactcttc caaagttctt tattatatat
    72661 tggcaccaca tctatatgat taagcacccc tactacttct tctccttctc catattctat
    72721 atgaattgca tatccatcaa agtcttttac aacaaaatct ttgtcttttg ctatttgaat
    72781 aagcttatcg aaggcatttc ttatttctat cccaaatggt tgatttatat ctgttgtact
    72841 ttcatctcta attgaaggta tttcaactaa acttctaata tcctttatga tattttcttg
    72901 attggatttt aaataatcaa taattagttc tttgtttact atcatcttaa ttatttatca
    72961 cccctttata ttaattttac aaagcatttc agttcatatc tacattataa attttgaata
    73021 ttattttttc aatatttcaa atacttctct aaattaccat aatgatacgg taaacaatca
    73081 acagattatg acaaaatcat actataccta ttactaatat tttaacttat ctattacgtc
    73141 ttttttaatg taattttatt ccaaaaatag atataaaaag atgtagcctt agctacatct
    73201 ttttatatct attcaacttc cattattatg ctattaatat ctacatttcc aggttctatc
    73261 ctatctttta ctgttctctt aacagaatca gcatcatcca ttataactac aggagttatt
    73321 atatttaaat catttgacgt tataaaatct aaatctactt ccattattaa atctttagct
    73381 tttaccttat ctcctgcctt taccttaaca tcaaacggct ttccttctag tgacattgtc
    73441 tctaaaccta catgtattaa tatatttgta ttatcattac tcttaatcat tattgcatgc
    73501 tttgtatcaa atacattgac tatttcacca tcaacaggag aatataactt accttcattt
    73561 ggtattattg ctactccatc tcccatcatc ttttgggcaa aaactgcatc tggaacatct
    73621 tctaagttca ctaaatttcc cttaaaaggg ctatatattt tctcaatctt attttctttt
    73681 ttcttaaata tattaaacat aacacactct ccatattata aaattttatg ataatatttt
    73741 tcttaaagct tttgctacac catcatttac attagtatca gttacaaaat ctgccataga
    73801 ttttacttca tctgtagcat ttcccatagc aataccaaga cctgcaaatt taatcatgga
    73861 tatatcattt tcattatcac ctatacaaat aacttctttg ctatctatac ctaatatatc
    73921 acaaaatttt ttaactgcaa taccttttga agtaccttta ctcataactt caaaatttat
    73981 ctttccagaa cttactactt ctaaatcttc ttgtttttta aattcttcct ttgcttctct
    74041 caaagaatct acgttttctg agaaaagtat acattttaaa atttcattat cagctttttc
    74101 taataaagtt tcacaacttg cattttctat tatttcaatt ctgtcttctt ctttattttt
    74161 actatttatt aatctatatg gatgttcttc acctatttca ttgttggata taactatttt
    74221 acagccttta aaatgtttgt ttaaattata cttttcacct attgtataga ttttctttat
    74281 agcttctttg ctcaaaacct tcttatactc atatcccatt aatttaaaat atgtcccatt
    74341 agttgatata atatatatat catcccctaa aacacttgta aaatatttca tagcattata
    74401 aggtcttccc gtaactaagg ctaccttgat acccttttcc atagcttctt ttagtgcttt
    74461 aatattttct tctgatactt caaatccttt ccccatcaaa gtaccatcca tatcagtaca
    74521 aattaattta tatcgcattt gtatcacatc ctagtaagat ttttctaata tttcaacaag
    74581 gtcttctttc tttccaaaag gtaaaaatcc taaaactgga cagcttgtta tttgttctaa
    74641 ttgttccttt gttacattaa actcagacat ttttgtctta agtcccactc tctttaaaaa
    74701 actttctatt tcatttccta aagttttagc tgcttcatca tcttccactt tttctaattc
    74761 ttggtttagc attctcgcta ctttggcaaa tttctctata ttatccttat agaacttttc
    74821 tgcaaatttt gggaacacta tagccaaagc ctcaccatga ggaatatgtg taactcctcc
    74881 tattatttca cttagtggat gtggtgctgc tgctccagca tttgccagtg aacttcctcc
    74941 taatgtatct gctactgcta actttgttct gtattctata ttatttttat cttccattac
    75001 tttaggtaga tagttaatga ctaattccat agatttttca gacattatct ctgtaaagtc
    75061 attagcattt ggatttatat aactttcaaa tgcatgagta aaagcatcaa atgctgttga
    75121 tgcagtaatt ctctctggca gagttaacat aagctctggg tcaactatac attcttttga
    75181 gaaacaattt tgatgaaata ttgtattttt ctcgcttcct ctcgatatta ctgctgcttg
    75241 tgtaacttgg cttcctgttc cagaagtagt tgatacagct attaatggta attctttttc
    75301 acttacctta tcgtatattt taaatggatt tgtatatgta ctaaacatat aatcccaatc
    75361 tattttttct aaaccaaaag tcaatgctaa tacttttgca gtatctatac ttgaacctcc
    75421 tccaacagct aaaacaacat ctacagattc ttttttagct aattcgaacc cttcatttat
    75481 taattctaca gttgggtttg gctctactct atcaaagtgt acaacttcta tacctttttc
    75541 tattaaattc aatgtaactc ttttatataa cttgtctaat ggctctacat ttggagtagt
    75601 aactaacaaa catttctttc catattttcc tattattcta ggtagttcac ttaaagttcc
    75661 ttgtccaaaa tttattcttg atggttgata aaagttaatc atatttttcc tcccttaaat
    75721 tattgacttc atattcaaat tatacatatc ataggatttt taaggcaata ccttcactgt
    75781 taactcttat ttactaaagc ttatggcaat tattagacaa taaatgtttt attattacca
    75841 tatcattatg taaattttat taagtaccca tgttataatt ttataatcaa tcttattgta
    75901 taattgactt aataataaaa atagagtata tacacttttt tatgacaaat agcttaaagg
    75961 agaattaaat tatgaaatat ttgatagaca gtgctaatct tgatgaaatt caaaagtgtt
    76021 taaagtatgg atttaaggga ataacagcta atccatctat gtatcttaaa gagaaggtaa
    76081 acttccatga atttattgag acatgtacta atttaaatcc agaaatatta actgctgaag
    76141 tcattggaaa taatttatac gaattaatta attcatgtga taagatatta aatataaata
    76201 aaaacactat aataaaaata aatttctctg aagttggctt agaacttata aacatattaa
    76261 atagtaaagg tgtaaaaact gcatgtacct tgattttcaa tgtaaatcaa gcatcattag
    76321 ctataaatgc aggtgctgat tacctattcc cttttatagg tagaaatgat gaaaatggga
    76381 atgatggtat aaaaaacctt acagaaatat gtaaccttat atcaataaat ggatataata
    76441 ctaaagttgt tgcagcttct attaaaaatg tattccactt aactcaagca tctctttgtg
    76501 gatgtgatta tgcagctgta acatatgatt tatttaaaaa agcttctttt aaccagctta
    76561 cagtagatgg tgccaatact tttgaaagag attggtccaa ggttaataaa tagtaactaa
    76621 gatttaggag ggtttgcatt gaacaaaaga ttattagata ttgctgaatt attactcaac
    76681 agctcagact atattactgt agatactatt gccgaagaat taaaagttag taataaaact
    76741 attcgtaatg accttgttat tttagatgaa tggcttttag agtttaattt atccctagat
    76801 aaaaaaactg gttcaggggt tattattcta ggtaatgaag atataaaact taaagttatt
    76861 agagatatca atgataaatc aaattgtata tatgcatact ctcctgaaga tagaaaaagg
    76921 tatattctaa gtaaattatt tattaaaaat tctaagttta ggattcgtga tatttgcaat
    76981 gaactacatg ttagtagagc tactgttcat aaagacttgg tagttattca aaattttttg
    77041 caaaacttta aagtgactct agttagaaaa accaataatg gagtatattt agagggaaaa
    77101 gagaaagata ttcgaaaagc agtttttgaa cttgctactg ttaataaaag ttatactgaa
    77161 cttaaagaaa tattattctc gaactctaat gaatgcaaag ataccccatc aacaaaaata
    77221 tttaaggagc tatttaacta tgattttgag aaattatctc aaatcactct acacacctta
    77281 caagtagaaa aaagtaacct tagtgatgag tattatataa acttcttaat acatatagct
    77341 atatgtatta agagaatttc aattaaaaga tatattaact tatctgaagg tttttttaga
    77401 gaattattag aacatgaata ctttgaaaaa tcaaaaattt tatgtgctaa cctaagtgaa
    77461 gcttttgacg tagaatttat tgatgaagag gtctgttata tacttcttca tatagaagga
    77521 ttacttaaat caataaagaa aactactact cttgaacctc ttaaaaatga agaaattaca
    77581 gagtataatc tatctgaagc aatagctgtt cattggggaa gtatactaaa tttaaactta
    77641 aaggatgatt taatattaat aaagtctctt gctaatcacc ttaaatcagt tctacacaga
    77701 ataaactatg gatttactat tacaaatccc atattaaatg atataaagag aacatttcct
    77761 tatacttata aggctgctaa agagtctaat actgttatca aacagttgat gaattatgaa
    77821 gtcaatgatg acgaaatagg atatcttgct ttatatttaa tatcagcaat agatagaagc
    77881 aaatctcccc taaatactat tttaatatgt cactgtagtg atggtgtatc taatttatta
    77941 gtccaaaagt taagttttga atttaatcag attaatatag ttaaatctat acctctatct
    78001 tctatttcat ttacgaactt tgatgatgtg gacttaatac taaccactgc tcctgtagac
    78061 tttgaacata atgctgaact gataaatata aatgctcttt taagtaagaa tgatatttcc
    78121 agacttagta caattataaa aaaactatat tctgaaaaaa ataaaatatt atcttataaa
    78181 taaacaaaaa taggcttgtc tcaacataga gataaaccta tttttcattg tccaaatgat
    78241 aaaaatatat ttgatttcat agaaaaagtg cctcatgaac taaaaaattc attttgagac
    78301 accttcattt tttatattta atctttttat ttaagctttt atatttttta aacttttata
    78361 ttttttaaac ttttatattt aaagtaaatt ttattatttt tattgagcgc aagcattatt
    78421 tatattatta atttcttcag tattcttacc aaactttgta gttacaaatc ctattgtttt
    78481 tattatgagt aataaggata tcaatgaata tatagatatc ataaaattct tacttattat
    78541 tccagatgct actattataa atccatctag cataaataat ataactggca acttcagaaa
    78601 cttaattacc ttattaataa gcattgcaac taaatcagtt ccaccagtac tacagcccct
    78661 tattattgct atccctatag cagttcctac caataaccca cctatcactc ctgctactaa
    78721 tagattgtca gttatgtgag tttgtggtat tacttttaag aataatggac ccataagagt
    78781 tatatataat gtttttattc caaaaccttt ccctaaaaat aataatccca ttactaaaag
    78841 tggcatagat attcctaatg atatattctc aacaggcatt gataagaatg tgctaagtac
    78901 gactgataat cctgttatcc ctcctggagc taatgtatgt tctgcaaaga aaaaattaac
    78961 tgctaatgcc attaaaaatg ctgatattgt tatcagtaaa taatctttta tcatttcttt
    79021 tttcatttaa taacctccta tgattatatt gtttcagtat aaaaaatcaa ttaaattagt
    79081 ctagcttcct gtagtttttc ataaaattaa tgcttcctta aaacattaag aaagcatctc
    79141 ttttatcata gtttattatc ttattctaaa ccaaactttg tagctgcttc atctatagct
    79201 tttctacaat tcttaacacc tgctgttata ccatctttat ccttgaatat tgctgaagat
    79261 aatattacaa cgtctggatt acattctatt aatggctcca tattgtctgc tcttagtcca
    79321 ccatctattg ctaattcaca cttaggattc ttttcgtcta tcatttttct agctcttttt
    79381 actaaatcaa cagcactctt tctccatccc cagttatctt ttccatcagt ttcatcaaca
    79441 ccatgaacga ctatttgcaa tctatctata tcatatatag cttcatcaac aaaacatagt
    79501 ggtgtgtaac atccaattgt taatcctact ttcataccaa attctctaca gtaatttatt
    79561 atgtatgcaa gaggagcgcc taaaaaatgt tctgctggta ttatcaacaa gttacatcct
    79621 gctgctgcta ttttttctat aaataatcta tcacaatctc ttgtgtatat atggcattct
    79681 attggtttat ctgtcactgg tcttattgct tctattattt gatgaccacc cataagttgc
    79741 atatttttta agtcatgcat atctgctgca tctgaatgta cgtagtctac acctgcatcc
    79801 attgcttctt taactacggc tgatatatga ccgtaattta catgtgctaa tcctgctgct
    79861 atttttatgt gtttcatatt tgtatacctc catattttac tttaattagt attaaatatt
    79921 agtttttgat attttttaat ccagtattac ttttatttag cattactttt attgctgtat
    79981 atatttcttt tatgaactta ttctgctttt gttaactgtg aacttatttt gctttgtaaa
    80041 ttaatattac actgatatat tttttttcac aacactttga ctactcctta aaagtacctt
    80101 tatattgtgg taaattatag acattaattg tatataaaaa gttagttttt taccacattc
    80161 ttatggcatt tattgtctat actaactcaa ttgatatagc tagttcattt aatatataat
    80221 cagactataa atataaattt actcgataaa aagtaccatt cataacaata ttaatgaaag
    80281 ggagatgtaa aatgagaaac tgtattattg cccaatctgg aggccctaca gctgttatca
    80341 atgcttctgc agttggtatt tatgaaaaaa atttagaaac taaatacttt gataaagtat
    80401 attttggtat caatgggatt gaaggtatat taaacaaaaa tataatagat tcagactcct
    80461 taaacaaaga taatgtatta aatttaaagc aaacaccttc gtctggtctt ggttcttgta
    80521 gatacaaact ggctgattat aaaataaatt cttatgatta tgatagagtt ataaatatac
    80581 tagatgaata tgaaatagat acattatttt atataggcgg aaatgattct atggacacag
    80641 tgatgaaatt ttctcaatac tgcaaaaaca ctacttgtaa aataaaattt atagggattc
    80701 ctaaaacaat agataatgat ttattaggaa ctgacaactg tcctggattt ggttctgctg
    80761 caaagcttat ttctacacta ataatggaaa catacttaga tttttctata tacaaaaata
    80821 atggtatatt tatagtagaa accatgggta gagatactgg atggttagca gcttcttctt
    80881 cactagctag acttaatgat actccacttg ttgatttaat ataccttcca gaaattgatt
    80941 tctatgaaga taagtttata gaagatgtta gaaaaatatt taaagaaaaa aataaggctt
    81001 atatagttgc atcagaaggt ataagggata aatacgggaa ctttatatgc gaacaaaaaa
    81061 cttctgctca tgataaattt ggtcattccc aattaggtgg agtaagcaac tatctaaaga
    81121 atcttatagt atcatcatca attacaacaa gagtaaaaac cttagaactt ggtgtaatac
    81181 aaagatgtgc tatgcattta gcttcagata ctgatttaaa acaagcagct gaagttggtg
    81241 catatgggat agaactatct aaaaaaggat atacaggagt tatggctatc attaatagaa
    81301 catgcaataa tccttataag tatgaagtta gtcatactga tatttctaaa atagctaaca
    81361 agacaaaata ttttcctaaa gagtggataa atgaagaatg taattatata actgatgaag
    81421 ccctaaatta tctatatcca cttattcaag gtaaaccaga tatcaaatat aaaaatggac
    81481 taccttattt ttcaattatt tactaggaaa ttattataat ttgatataca ctcaattcta
    81541 tcgctcttaa atacctaagt ggctgttaaa taaaattata aattgttata tgacagtcac
    81601 ttaatcgttt aattatttaa ctgtttagtt ttttaatttt ttaatctttt ttaatactaa
    81661 tacgatttgt tgtaataata tctcacaccc attttcattt tggatagatt ataattagtt
    81721 agacaataaa tattttgtca tgatattatt atatatagtg atacaaatag aaatactttt
    81781 atcatgacta gaaaaacaaa gattacacaa aggatttaaa aatcaaacaa cttcaattaa
    81841 aataaatggt taaaataact tctaaatcaa ctacataaac
//
LOCUS       DAELXX010000003        77124 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.003, whole genome shotgun sequence.
ACCESSION   DAELXX010000003 DAELXX010000000
VERSION     DAELXX010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 77124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77124
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.003"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            105..434
                     /locus_tag="KPH29_000146"
     CDS             105..434
                     /locus_tag="KPH29_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1465791.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
     gene            438..1229
                     /locus_tag="KPH29_000147"
     CDS             438..1229
                     /locus_tag="KPH29_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1465792.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTNIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYEREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFAFGLLCIIAYFILRFKISLIKNNN"
     gene            1793..2875
                     /locus_tag="KPH29_000148"
     CDS             1793..2875
                     /locus_tag="KPH29_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBG1465793.1"
                     /translation="MKKIRKLATKLLIATMVLTSCMSVTVYGMTAKDVTAKTPKSYVA
                     GTNSIYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKIRVAYNHIKNNTTYVNWNA
                     AEGANTAYTLVTKKGACSGMCRSLKALCDAMGIECYYVHSTTNDHQWNLIRFEDGRLY
                     HVDIDANKSAGADIIYKSLTHPLPFDKKAYPAVGESVKTQNPTPTVKKPTPAKPSAPV
                     GPGNHTMIADQEEAILDEVYGNNLRPKIERYEGVPCSLINSSINTIKFTSKNSNLDPS
                     RHTVSVIGFMNTPYGMVALAPDGVPVEYPFEYDKPFSLDFTGKNLSAAELSKKCKNNN
                     VIIKIEIGEIEPNSGRGQVRYARYVN"
     gene            complement(3087..4280)
                     /locus_tag="KPH29_000149"
     CDS             complement(3087..4280)
                     /locus_tag="KPH29_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HBG1465794.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
     gene            4599..6443
                     /locus_tag="KPH29_000150"
     CDS             4599..6443
                     /locus_tag="KPH29_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HBG1465795.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFI
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
     gene            6445..7146
                     /locus_tag="KPH29_000151"
     CDS             6445..7146
                     /locus_tag="KPH29_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HBG1465796.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGTESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            7164..8585
                     /locus_tag="KPH29_000152"
     CDS             7164..8585
                     /locus_tag="KPH29_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HBG1465797.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLIIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            8686..8997
                     /gene="rplU"
                     /locus_tag="KPH29_000153"
     CDS             8686..8997
                     /gene="rplU"
                     /locus_tag="KPH29_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBG1465798.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            9012..9329
                     /locus_tag="KPH29_000154"
     CDS             9012..9329
                     /locus_tag="KPH29_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBG1465799.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVKEIKREYPKNIKI
                     LKK"
     gene            9343..9633
                     /gene="rpmA"
                     /locus_tag="KPH29_000155"
     CDS             9343..9633
                     /gene="rpmA"
                     /locus_tag="KPH29_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBG1465800.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            9744..11021
                     /gene="obgE"
                     /locus_tag="KPH29_000156"
     CDS             9744..11021
                     /gene="obgE"
                     /locus_tag="KPH29_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBG1465801.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            11073..11441
                     /locus_tag="KPH29_000157"
     CDS             11073..11441
                     /locus_tag="KPH29_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HBG1465802.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     regulatory      11533..11639
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            11742..12947
                     /locus_tag="KPH29_000158"
     CDS             11742..12947
                     /locus_tag="KPH29_000158"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBG1465803.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SAKKLLKID"
     gene            complement(13148..13714)
                     /locus_tag="KPH29_000159"
     CDS             complement(13148..13714)
                     /locus_tag="KPH29_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG1465804.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKHLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(13982..15241)
                     /locus_tag="KPH29_000160"
     CDS             complement(13982..15241)
                     /locus_tag="KPH29_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HBG1465805.1"
                     /translation="MKKNKIIAFFAPAIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKHEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFIKKFSRKK"
     gene            15366..16079
                     /locus_tag="KPH29_000161"
     CDS             15366..16079
                     /locus_tag="KPH29_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBG1465806.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            16197..17471
                     /gene="larA"
                     /locus_tag="KPH29_000162"
     CDS             16197..17471
                     /gene="larA"
                     /locus_tag="KPH29_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HBG1465807.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            17488..18276
                     /locus_tag="KPH29_000163"
     CDS             17488..18276
                     /locus_tag="KPH29_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG1465808.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEDKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKDENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            18290..19510
                     /locus_tag="KPH29_000164"
     CDS             18290..19510
                     /locus_tag="KPH29_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HBG1465809.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            19521..20912
                     /locus_tag="KPH29_000165"
     CDS             19521..20912
                     /locus_tag="KPH29_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBG1465810.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDPKNILNPGKIY"
     gene            complement(21175..22455)
                     /locus_tag="KPH29_000166"
     CDS             complement(21175..22455)
                     /locus_tag="KPH29_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBG1465811.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNIAEILANEPNEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKVDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDM
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSNISFSLDDTIFKTLISY
                     DIKATNIYNSIYYKNNKDLVKGPMDFYTHPIYVK"
     gene            22918..24114
                     /locus_tag="KPH29_000167"
     CDS             22918..24114
                     /locus_tag="KPH29_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBG1465812.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
     gene            24207..24731
                     /locus_tag="KPH29_000168"
     CDS             24207..24731
                     /locus_tag="KPH29_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBG1465813.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDTCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            24756..24932
                     /gene="rpmF"
                     /locus_tag="KPH29_000169"
     CDS             24756..24932
                     /gene="rpmF"
                     /locus_tag="KPH29_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBG1465814.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            25139..25696
                     /gene="fapR"
                     /locus_tag="KPH29_000170"
     CDS             25139..25696
                     /gene="fapR"
                     /locus_tag="KPH29_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HBG1465815.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            25707..26729
                     /gene="plsX"
                     /locus_tag="KPH29_000171"
     CDS             25707..26729
                     /gene="plsX"
                     /locus_tag="KPH29_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBG1465816.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            26755..27741
                     /locus_tag="KPH29_000172"
     CDS             26755..27741
                     /locus_tag="KPH29_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBG1465817.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            27850..28779
                     /gene="fabK"
                     /locus_tag="KPH29_000173"
     CDS             27850..28779
                     /gene="fabK"
                     /locus_tag="KPH29_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HBG1465818.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            28795..29745
                     /gene="fabD"
                     /locus_tag="KPH29_000174"
     CDS             28795..29745
                     /gene="fabD"
                     /locus_tag="KPH29_000174"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBG1465819.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNAVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQIDLKYNACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQETVPAGVGAMAAILGLDRNVLENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLKEAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIEILLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            29745..30494
                     /gene="fabG"
                     /locus_tag="KPH29_000175"
     CDS             29745..30494
                     /gene="fabG"
                     /locus_tag="KPH29_000175"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBG1465820.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTRVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            30548..30772
                     /gene="acpP"
                     /locus_tag="KPH29_000176"
     CDS             30548..30772
                     /gene="acpP"
                     /locus_tag="KPH29_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBG1465821.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            30801..32039
                     /gene="fabF"
                     /locus_tag="KPH29_000177"
     CDS             30801..32039
                     /gene="fabF"
                     /locus_tag="KPH29_000177"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBG1465822.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADIMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            32265..33983
                     /locus_tag="KPH29_000178"
     CDS             32265..33983
                     /locus_tag="KPH29_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG1465823.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIILFIKYDQNGLKEREIINKG
                     KYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLNIGSYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGEY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMKSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGEEVADIIDNIRVAIANKKWNDDVTVTISAGVAHISDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            34014..36014
                     /locus_tag="KPH29_000179"
     CDS             34014..36014
                     /locus_tag="KPH29_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG1465824.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKEN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKEKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDIISEDLNILLEDKEIIDE
                     VVFTNKKYINVNKKSIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMVKCVELAKKISKSDLSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLLKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNVRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYIRVECANNYEDGFGYTKKEFEEILRILYERRESSSGVGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            36362..36607
                     /locus_tag="KPH29_000180"
     CDS             36362..36607
                     /locus_tag="KPH29_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBG1465825.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            36642..37367
                     /locus_tag="KPH29_000181"
     CDS             36642..37367
                     /locus_tag="KPH29_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBG1465826.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEEENFVVGVQWHPE
                     MMVDYCDKMEKLFKYFINICSNL"
     gene            37389..38702
                     /locus_tag="KPH29_000182"
     CDS             37389..38702
                     /locus_tag="KPH29_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG1465827.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVAMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKVCGENLKKIV"
     gene            39001..39447
                     /locus_tag="KPH29_000183"
     CDS             39001..39447
                     /locus_tag="KPH29_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG1465828.1"
                     /translation="MGGETHFLSFGKDEYKISLEEQENAIESAKASDNSVKLIAFIDD
                     EIVGIATIDSNQKAKGKHVGVLGIVVKEKYWGIGLGKRLMLDLIEWCKSNGITKKITL
                     VTNEENYNAIGLYKKVGFEVESILKKECYYNGVYTDLIGMSLLLGI"
     gene            39536..41521
                     /locus_tag="KPH29_000184"
     CDS             39536..41521
                     /locus_tag="KPH29_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HBG1465829.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTITLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIEEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGVKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            41745..42713
                     /gene="spoIIIAA"
                     /locus_tag="KPH29_000185"
     CDS             41745..42713
                     /gene="spoIIIAA"
                     /locus_tag="KPH29_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HBG1465830.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIDD
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            42703..43224
                     /locus_tag="KPH29_000186"
     CDS             42703..43224
                     /locus_tag="KPH29_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HBG1465831.1"
                     /translation="MQIKIIIIALLVGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            43241..43438
                     /gene="spoIIIAC"
                     /locus_tag="KPH29_000187"
     CDS             43241..43438
                     /gene="spoIIIAC"
                     /locus_tag="KPH29_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HBG1465832.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            43454..43840
                     /gene="spoIIIAD"
                     /locus_tag="KPH29_000188"
     CDS             43454..43840
                     /gene="spoIIIAD"
                     /locus_tag="KPH29_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HBG1465833.1"
                     /translation="MEIMQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVM
                     FKLNFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEF
                     GGKIIVMSMSFPILLSIVEMVVNIIP"
     gene            43925..45130
                     /gene="spoIIIAE"
                     /locus_tag="KPH29_000189"
     CDS             43925..45130
                     /gene="spoIIIAE"
                     /locus_tag="KPH29_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HBG1465834.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLIGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            45190..45807
                     /locus_tag="KPH29_000190"
     CDS             45190..45807
                     /locus_tag="KPH29_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HBG1465835.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            45819..46373
                     /locus_tag="KPH29_000191"
     CDS             45819..46373
                     /locus_tag="KPH29_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HBG1465836.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKKQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            46400..47089
                     /locus_tag="KPH29_000192"
     CDS             46400..47089
                     /locus_tag="KPH29_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HBG1465837.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDDSDSSKTEDSSNVDKQGGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            47199..47561
                     /locus_tag="KPH29_000193"
     CDS             47199..47561
                     /locus_tag="KPH29_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBG1465838.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            47646..48152
                     /gene="nusB"
                     /locus_tag="KPH29_000194"
     CDS             47646..48152
                     /gene="nusB"
                     /locus_tag="KPH29_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBG1465839.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            48136..49206
                     /locus_tag="KPH29_000195"
     CDS             48136..49206
                     /locus_tag="KPH29_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HBG1465840.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGDNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSMKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            49203..50408
                     /locus_tag="KPH29_000196"
     CDS             49203..50408
                     /locus_tag="KPH29_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBG1465841.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVMVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            50410..50625
                     /gene="xseB"
                     /locus_tag="KPH29_000197"
     CDS             50410..50625
                     /gene="xseB"
                     /locus_tag="KPH29_000197"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBG1465842.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            50628..51515
                     /locus_tag="KPH29_000198"
     CDS             50628..51515
                     /locus_tag="KPH29_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBG1465843.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIEKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            51545..51982
                     /locus_tag="KPH29_000199"
     CDS             51545..51982
                     /locus_tag="KPH29_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HBG1465844.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            52016..53881
                     /locus_tag="KPH29_000200"
     CDS             52016..53881
                     /locus_tag="KPH29_000200"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBG1465845.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANNNED
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDDGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            54014..54826
                     /locus_tag="KPH29_000201"
     CDS             54014..54826
                     /locus_tag="KPH29_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HBG1465846.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEYTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            54846..56525
                     /gene="recN"
                     /locus_tag="KPH29_000202"
     CDS             54846..56525
                     /gene="recN"
                     /locus_tag="KPH29_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBG1465847.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKNVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLKSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGSFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            56740..57606
                     /locus_tag="KPH29_000203"
     CDS             56740..57606
                     /locus_tag="KPH29_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1465848.1"
                     /translation="MNKKDTMHLIFIIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QELMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECTIDVDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKHGELSYACEIKINTGSEYDYKVVTSGDISESSDVQKLTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKAYDNIRLEKVDIIVNEGKKD
                     TIYKAQLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDDIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(57697..58188)
                     /locus_tag="KPH29_000204"
     CDS             complement(57697..58188)
                     /locus_tag="KPH29_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG1465849.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKI
                     GKILLEEIIVEAKKIGYTHMRLDTLPSMKSAQGLYEKFGFYDIELYTYNPIEGARYME
                     LKL"
     gene            complement(58249..58689)
                     /locus_tag="KPH29_000205"
     CDS             complement(58249..58689)
                     /locus_tag="KPH29_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG1465850.1"
                     /translation="MKLNVRIADINNWHDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGCGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFKFNGELDYGGEKVMVLKSN"
     gene            58850..59908
                     /locus_tag="KPH29_000206"
     CDS             58850..59908
                     /locus_tag="KPH29_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HBG1465851.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVGYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGDAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            60050..60874
                     /gene="spo0A"
                     /locus_tag="KPH29_000207"
     CDS             60050..60874
                     /gene="spo0A"
                     /locus_tag="KPH29_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HBG1465852.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            61287..62408
                     /locus_tag="KPH29_000208"
     CDS             61287..62408
                     /locus_tag="KPH29_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1465853.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            62426..63301
                     /locus_tag="KPH29_000209"
     CDS             62426..63301
                     /locus_tag="KPH29_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HBG1465854.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFNILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIVLKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNSLGNTYLKYDSVVIAGGNDAKDSTKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            63325..64017
                     /locus_tag="KPH29_000210"
     CDS             63325..64017
                     /locus_tag="KPH29_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG1465855.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            64025..64849
                     /locus_tag="KPH29_000211"
     CDS             64025..64849
                     /locus_tag="KPH29_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBG1465856.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKDLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIIFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            64854..65921
                     /locus_tag="KPH29_000212"
     CDS             64854..65921
                     /locus_tag="KPH29_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HBG1465857.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGKIQRAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            65923..66459
                     /locus_tag="KPH29_000213"
     CDS             65923..66459
                     /locus_tag="KPH29_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG1465858.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            66527..67132
                     /locus_tag="KPH29_000214"
     CDS             66527..67132
                     /locus_tag="KPH29_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HBG1465859.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKNR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTVSIGIIKFLAK"
     gene            67144..68058
                     /locus_tag="KPH29_000215"
     CDS             67144..68058
                     /locus_tag="KPH29_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG1465860.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAVEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            68100..69272
                     /locus_tag="KPH29_000216"
     CDS             68100..69272
                     /locus_tag="KPH29_000216"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBG1465861.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIDGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            69319..70131
                     /locus_tag="KPH29_000217"
     CDS             69319..70131
                     /locus_tag="KPH29_000217"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBG1465862.1"
                     /translation="MFEKIAQSSKFINSKSNIKPKIGLILGSGLGDLANDIEDPVTIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            70335..71660
                     /locus_tag="KPH29_000218"
     CDS             70335..71660
                     /locus_tag="KPH29_000218"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBG1465863.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEVDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSIKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
     gene            71873..72952
                     /gene="mltG"
                     /locus_tag="KPH29_000219"
     CDS             71873..72952
                     /gene="mltG"
                     /locus_tag="KPH29_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBG1465864.1"
                     /translation="MNFKENRLKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIID
                     VPSGASVGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFPYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKATNKENTKK"
     gene            73124..73798
                     /locus_tag="KPH29_000220"
     CDS             73124..73798
                     /locus_tag="KPH29_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBG1465865.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFASIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRNYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
     gene            73785..75032
                     /locus_tag="KPH29_000221"
     CDS             73785..75032
                     /locus_tag="KPH29_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBG1465866.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEISEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRAPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            75104..76768
                     /locus_tag="KPH29_000222"
     CDS             75104..76768
                     /locus_tag="KPH29_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBG1465867.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDKSMKKELEKNGIMVEEKTMRYAKDGLLS
                     HTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKTVD
                     NKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQNDIS
                     KSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNTNE
                     ILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGLDE
                     EKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNMNT
                     IKTYKSSKKEELISPYVCTQVKEYMKSVSRIGTAKDLKDIEGGCGVKTGTAQSSLNKK
                     AIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(76865..>77124)
                     /locus_tag="KPH29_000223"
     CDS             complement(76865..>77124)
                     /locus_tag="KPH29_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG1465868.1"
                     /translation="ILGTEANYVLDEVELKVQVGKLYEQLNKILTPREREIVQLRYGL
                     TPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
BASE COUNT        30946 a         8031 c        14136 g        24011 t
ORIGIN      
        1 ctattgtaga atataaacct tgtattatag agtactatgt attattatag taaatataag
       61 tactatacat tatagagtag taggtgaaaa ggcggtggta cataatgcag ttaaataaag
      121 aagtgttaaa aggttatatc gatatattaa ttgtttctat attagaaaag aaagattgtt
      181 atggatatga aattgcaaag caagtaagag aaagaagtga gtttgaatta aaagaaggaa
      241 caatgtatct tgcacttaaa agaatggaat ctaaaaattt aattaaatct tattatagca
      301 atgaacaaag ttcaggtgga agaagaaaat attataattt aactaatgaa ggtaaagatt
      361 ttttagaaat aaagaaacaa gaatggagat ttattaaaaa ggttatgaat caatttttgg
      421 gagaggtgga ttaatttatg aaactaatag atgaatattt agataaacta tataaaaaat
      481 gtgacaataa aagtactata gaattaaaac aggaaatgag atgccatctt atagaatctg
      541 caaatgaatt taaattagaa ggattagatg aagaagaagc atgtaaaaaa gcaattgaaa
      601 gatttgatga tggtgatgaa atgcagtatg aattatgtaa tataataaaa gaactatctt
      661 tatcattgga taggcataaa tcaattgtaa tggggtttaa aaaagtatta ggttacataa
      721 gtataattgc atttttaatt tctggattta tgtggtatta caataatagt ttacaacata
      781 atatgtataa tttaggaaaa gaacttgatg gagaaataaa gcaattggca gaaagacatg
      841 atatgacaaa tattggtgaa tataagttag aacttgaaaa aatacttgat aaagataagt
      901 atagtaaagt caaagctctt agactatatg taatagatat gaaagatgga aacactaatt
      961 taagttcttc tggattgaat gctaatatgg tatatgagag ggaagcagat tataataaca
     1021 taagtaattt tatacagcat ttaggatata atggaaaaga ttttttagac aagaatggga
     1081 atatagtaaa tccagatatt ttcttagaat atttctttta ttttgaatcg gagatgttaa
     1141 ttccagtagc atttgcattt ggattattat gtataatagc gtattttata ttaagattta
     1201 aaatttcttt aataaaaaat aataattaaa taattcacca aataatttta atttgtagag
     1261 taaataagat gtttcaaaat aaaatttgaa tcatcttatt ttttattgtt tgttatactc
     1321 ttttctttta tatttctcca tagtggattg tatctataca taatcacata ttttgatata
     1381 attatacctt ccaaaaaatg tattgaatat acaaattata atctgtataa tgttgttagg
     1441 ggagaatgta tgaagtgtat acaattttaa ttttaaatgt attattgaca attctcctca
     1501 ctctcgtcat taccaataat tagaaaattg acaaacctgt tacagaccaa catttttgaa
     1561 tatattaatt attatcattt tattgtgatg atatcaaaaa tgcaggtttg tgagttggat
     1621 tgtgtacgaa ctatatagga aatatagaac agattttcca agaaatctaa atattctatg
     1681 accacgtgaa ttgctatgca cacatgttga taatccatat tttaaatcta aaatgaactt
     1741 aattaatggt agggaattga aagtaaaata tattataaag gagaatgcta atatgaaaaa
     1801 aatcaggaaa ttagctacta aactattgat tgcaacgatg gttttaacct catgtatgag
     1861 tgtaacagtt tatggtatga cagctaaaga tgttactgca aagacaccga aaagctatgt
     1921 agctggaacc aacagtatat atggtccaaa gttatcacaa gctcaactta attcggtagc
     1981 acaagcaaca gcagacttta tgaacaagaa aattacaaag aacatgacca cagatgctaa
     2041 gattagggtt gcttataatc acattaaaaa caacacaaca tatgttaact ggaacgcagc
     2101 agagggagcc aatactgctt atacattggt tactaaaaaa ggtgcttgtt ctggaatgtg
     2161 taggagcttg aaagctttat gtgatgctat gggaattgaa tgttattatg tacatagtac
     2221 aacaaatgac catcagtgga atcttattcg ttttgaggat ggacgtttgt atcatgtaga
     2281 tattgatgcc aacaaatcag caggagcaga catcatatac aagagtctta ctcacccatt
     2341 accatttgac aagaaagcct atccagctgt tggtgaatca gtaaagacac agaatccaac
     2401 gccaactgtt aagaaaccta cgccagctaa gccttctgcg ccagttggac ctgggaatca
     2461 cactatgata gcagatcaag aagaagcaat acttgatgaa gtttatggaa ataatctacg
     2521 tcccaaaatt gaaaggtatg agggagttcc gtgtagttta atcaactcat caataaatac
     2581 aattaagttt acaagcaaaa attcaaattt agacccatca aggcatacag tttctgtaat
     2641 agggtttatg aatacaccat atggtatggt tgcattagct ccagatggtg tacctgttga
     2701 gtatcctttt gaatatgata agccattttc tttagatttt actggtaaaa acctttctgc
     2761 agcagaatta tcaaaaaagt gtaaaaacaa taatgtaatt attaaaatag aaattggtga
     2821 aatagaacct aattctggaa gagggcaagt aagatatgct agatatgtga attaatataa
     2881 atatacaagt atacaaatag aaaagattat aaataatgta ccctcttggt gagggtgcat
     2941 ttatcattat attagtcatt ttgggaaata aaaaaggagt tgaacttaaa attagattag
     3001 aaaaattaat tttgagacaa ctccatttta atttattttt aaatataaaa tcatttattt
     3061 taaataatat tatgtgtgat taatggctat ttaactaatt ttgcaaattc tgcaccaaat
     3121 gccatacact cttttaatgt ctcttcacta gggaagaatt ttttcttaag agattctaca
     3181 ggcattttga atgacataga tttaagtaaa gtctcagcca tagttatacc ttcaccactc
     3241 caaccaaatg aaccaaatac tcctgctatc ttgccttggt tagccattgg gtctataaca
     3301 gagaataaat cccacatagg tttaaccata gttttgttta tagttggaga acctagtaat
     3361 attacttttg acactactaa tgtatcgtgc atttctgtta atgtcatatc ttctaaatca
     3421 tataattcag cctttgcacc ttctttatca agtccttctt ttatcttttt agccatctct
     3481 aaagtattac tataagctga taagtagaat atagaaactt gattttgatt agttgtatta
     3541 acagcttctg tagaccactc aacatatctt ttaacagcag ccattgggtc tttagtaagc
     3601 attggaccat gagaagtcaa tatagtatca aattctatgt ttagtcctac caccttatct
     3661 acagcactta aaacatgttt agcaaatggc ttaactatac aatcatagta gtgtttagct
     3721 gattttagat aatcttcact attcactacc tctgcatcaa cacttgcaaa gtgacaacca
     3781 aatgcatcac aagttaaaag agtcttgtcc tcctcaatgt aagtaaacat tgtatcaacc
     3841 caatgtaaga atggagcagt tataaatcta agatttcttt taccgatatt taaaatttca
     3901 ccatctttta ttacatgaca gttaaaaggt ctatttattt gaccatctaa gtatagttta
     3961 gcagctttag tacaataaac ttctatatta ggatttatat ctaataagta ttttaaacta
     4021 cctgcatggt ctggttctgt gtgatgaatt acaacatagt caattttagc tatatcagta
     4081 acctctgata aattacttaa aaactcatct ttaaaatttg cctttacagt gtcaaataaa
     4141 accgtttttt cgtccttgat taaataagag ttgtaagttg tcccaaactc agtttccatt
     4201 attatgtcga atacttttaa accttcatct actacgccag taaagtagat atcttttttt
     4261 acttcaaaaa ctttactcat ttttaaccct cctaaaattt atcattatat aagtaacata
     4321 cccaattttg aggtgaatga aacaaaagat gtcatgataa gtcatgtaaa acaatagttt
     4381 tatgtttata atatatcata ctttgtaaaa aaattgtatt tcaataaaaa tttaatagaa
     4441 aatattctta aatattttta gctattctat tttgtgaata atatgtattt taaattcaat
     4501 attttagtta tatttttgat agaaaatatt ttatcgtgta taatactact tataaataag
     4561 aataaaaata aagagcttgt ttggaggaat tttaataaat gaataaagta gacttgaaaa
     4621 aaatcttaaa aaaagtagaa aagcctgcta gatatcttgg aaatgaaata aattcaattc
     4681 ataaagatac tagtaatagt gaattgataa gatatgcaca ttgctttcca gatttatatg
     4741 aagttggtat gagtcactta gggagtcata tattatatga tgtaataaat aaggatgaag
     4801 atgtattttg tgagagagtg tattctcctg cagttgatat ggaaaatata atgagagaaa
     4861 aaagtatacc attatttgca ttagagtcaa gagagcctat aacaaacttt gattttgtca
     4921 cttttacact tcaatatgaa ctttcataca ctaatatctt aaatatatta gatttagcca
     4981 atataccaat attaaaagaa gaaagaactt tggaagaccc atttataatg gttggtggtc
     5041 cttgtgctta taattccgaa ccattagcag attttataga tgtagtaata ttaggtgaag
     5101 gtgaagaggt taatttagaa gtagtaaatg agtataaaga atggaagaaa aataaaacga
     5161 ctagagaaga ttttttatat aaaatatcta gcatagaagg agtatacata ccaagttttt
     5221 atgatgtcaa gtacaatgaa gatggtacag ttcaaagtgt aactcctaat agagaaggga
     5281 ttactaaaaa tcctactaaa aggataataa aagatgttga gactgtagat tatccagaaa
     5341 aactaatagt accttatata gatactgttc atgatagaat agtactagaa ttatttagag
     5401 gatgtactag aggatgtaga ttctgtcaag caggtatgat atacaggcca ataagagaaa
     5461 agagtgttaa aagacttaaa gaaatattag ataaattagt taagaatact ggatatgatg
     5521 aaatatcatt atcatcactt agtacgagtg attatagtaa gttatcagag ttgactgatt
     5581 atttggtaga tgaatatgca tctaataata ttgggatttc gcttccatcc cttagattag
     5641 ataatttttc aatggaaatt gctgataaaa tacaacaagt aagaaaatct ggacttactt
     5701 ttgctccaga agcaggaact caaagactta gagatgttat aaataaaggt gttacagaag
     5761 aagatttgga aaatgcaaca gaaagagctt ttgagatggg gtggaatagt gttaagttgt
     5821 attttatgat tggtttacca actgagactt atgaagattt agatggtata gctaaattag
     5881 cttataaagt tattgatatt tatagaaaag taaatggagg aaagctaaag agaagtttta
     5941 gtgttacagt gagtacttct acatttgtac caaagccatt tacacctttc caatggcatg
     6001 gtcaagatac tacagaagaa gttattaata agcagagaca tttagtaaat aaattaagaa
     6061 ataacaatat aaaatacaat tatcatgatt caaagactag tttgatggaa gcagtagtag
     6121 ctagaggcga tagaagaata ggaaaagtta tatatgatgc atttagacta ggtgctaaat
     6181 ttgatggatg ggcagaacat tttaatttag atatatggaa agaagctatg gaaaagaata
     6241 atctttctat agatttttat gcacatagaa atagaaacta tgaagaggta tttccttggg
     6301 accatataga tgtaggtatt agtaaaaaat tcttaattag agaagacgaa aatgctaaaa
     6361 aagaaaaaat cacttctgat tgtagacata actgcaatgg ttgtggaata aatatacatg
     6421 atataggaag ggggctttgc taatatgagt aagataataa gagtaaaatt taaaaaggaa
     6481 ggcgacatga tatatatatc acatcttgac cttcagagat tgttacaaag agcgtttaga
     6541 agagctgaaa taaacttatc acattctcaa ggatttaatc ctcaccctaa aatgagttat
     6601 ggcaatgctt tggctttagg gacagaaagt caaggtgagt atgtagatat tgaaatagaa
     6661 gaagatgatt tatcagtaga ggaattttta aataaagtat caattcaact tccagatggg
     6721 atagatttta tcaaagcaaa agaaattgat agacaaacac cttctctttc ttctgtgata
     6781 gactatggag aatatttgtt taatatagat ttgaaaagac ctatgacgaa agagtttgta
     6841 aaaagaaaag taattgactt tatgaataat aaagaaataa tcataacaaa aaagaataaa
     6901 aaaggtaaga tggttgaagt agatataaga cctatgataa gaacctttga tgttttaaat
     6961 ttagaagatg aacatattac tcttactgct actatagcta caggctcaaa aactaattta
     7021 aatactaata tacttatacc aaagatatta gagatgtttg aacttgatat tgacccatta
     7081 gatgttgata tattgagaag agatttatat gtattagaag atggggaatt agtgactcct
     7141 atgtaagctc gagaggtgaa gttgtgaaaa agatagttat tgagtcccta atagggtccc
     7201 agaaaacagc agtacttgaa gatgaaagat taacagaact ttttgttgaa gacaatttaa
     7261 ataagaagac tgtatccaat atatatcgtg gaatcgtaaa aaaagttata ccaggaatag
     7321 aagcttgttt tgtagatatt ggatttaaga aacttgctta tttacaatta aaaaagggaa
     7381 gtactataaa atctggtcag gatattttgg ttcagataaa taaagaagaa ataggaacta
     7441 aaggcgcaaa attaaacact gagattagta tttctggaag gtatattgtc tatatacctt
     7501 ccaatgatag aactacaata tcaaataaaa ttactgatga aaaagaacga tttagattaa
     7561 aaaaaattac taaggcagta aataaggaaa atctaggatt aataataagg actgaggctc
     7621 aaggttgcac tcatgatgaa ataaagaaag atatagaaga gttgaaatta aagtatgaaa
     7681 atattttaaa agaatataaa cttggtatag gtccgaaatt attatataag tctttagatt
     7741 ttgcaactaa atatgtaaaa gataatgtga atgatgatat tgaatctata attaccaata
     7801 gttatgataa gtactctgaa cttaagtcta ttttaagagg tatagataag acttatgttg
     7861 ataaactgtg tcttgaggaa aatagagatg tatttgattt atacagaata gaatctaaaa
     7921 tagaaaaatt attaaataaa aaagtatggt taaagagtgg aggttattta ataattgaaa
     7981 aaacagaagc tcttacggtt atagatgtaa atacgggtaa gtttattgga actggtaaac
     8041 ttgatgaaac cgtttataaa actaatcttg aagctgcaaa agaaattgta agacagctta
     8101 taattagaga catagctggg ataataataa tcgattttat tgatatgcat aaaaagaagc
     8161 atcaaaagga gattttacac atcctagaag aagaatttaa taaagataaa agaaaagctg
     8221 aagtattagg tatgacaaaa cttggtttag tagaagttgc taggcgtaga gaaaaagaat
     8281 ccattgataa gtattattta atgtcttgtc catgttgtga tggtgaacaa acaataaaat
     8341 ctgtacatta tatactagat agtattgaaa aagaaattat gagaataagt gaacatactg
     8401 tttacaaaaa cataatggta gaatttaatg attttatttt tgaacaaata aaagaatatt
     8461 atatggatat aattgataaa attggtgaaa aatataatat aaagatttct ttaaacgcaa
     8521 atagtacatt aaagcacaat aaaaccaatg ttattttcga taaaatagtt gacaataaaa
     8581 tgtaaatatt ttataattaa taattgtaat gccgcacagt aaaggtttaa atcttcaaat
     8641 gaagtacctt gtactggcga gtttgaggcc gaggaggtgt atttaatgta cgctatagta
     8701 aagactggtg gaaagcaata taaagtttct gaaggtgatg tattattcgt tgaaaaatta
     8761 gaagctaacg caggtgatgt tgttacttta aacgaagtat tagcttgctc taaagatgga
     8821 gaattaaaat taggttctcc agtagtagaa ggagcatctg ttcaagctaa agttgttgaa
     8881 caaggtaaag ctaagaaagt tatagttttc aagtataaag caaaaaaaga ttacagaaga
     8941 aaacaaggac atcgtcaatc atacactaag atagttgtag aaaaaataaa cgcttagtta
     9001 ttggttaagt tatgataaaa gttaaatatt attataatga tgatttcttg ttgcaaggtt
     9061 tttgtttaaa gggacatgct gattttgctg aaataggcta tgatatagta tgtgctgcag
     9121 ttacatctaa tgctattgca gttataaatt ctttagacaa attacaaaag attgaatttg
     9181 aaaaagtagt aggagaagaa ggtcatatag agtgtatagt caaagatacg catgtaaaag
     9241 acgcacaatt attactaaat cactttcaat tagcggttaa agaaattaaa cgggaatacc
     9301 caaaaaatat aaaaatttta aaaaagtagg gggtgactcc atatgttaaa catgaattta
     9361 caattattag catctaaaaa aggggtaggt tcttctaaaa atggtagaga ttctatatct
     9421 aaaagattag gtgttaagag attcgatggt caattagtaa ctgctggaag tataatagtt
     9481 agacaaagag gaactaaaat acatccagga actaatgttg gaaaaggtag tgatgatact
     9541 ttatttgcat tagttgatgg tactgttaaa ttcgaaagaa aagacaagaa gagaaaaaaa
     9601 gttagtatat atccagtagc aatagctgag taatatacat aaaaggagtg ttgaggcact
     9661 cctttttctt ataatactga tgaatttata atggtatttt tggtaatgct aattaaaagt
     9721 ttttaattaa gtaggtgaag aatttgttta tagataaggc aagaatattt gttaaggctg
     9781 gaaatggtgg aaatggttcg gtagcattta gaagagaaaa atacgttcca gctggtggtc
     9841 cagacggtgg agatggtggt cgtggagcaa gcatcatatt tgaagttgat ttggggctta
     9901 gaactctaat ggactttaag taccagaaaa aatatcaagc tcaaaatggt ggcgacggaa
     9961 gcaaaggaaa aagagctggt aaaaatggag aaaatttagt attaaaagta ccagcaggta
    10021 cagtaataag agatgaggct acaggtcttg tattagcaga tttaaaaaaa gaaggagata
    10081 cagctatagt tgcaaaaggt ggtataggtg gtaaaggtaa ccaacacttt gcaaatgcag
    10141 taagacaagc tccagctttt gcaaaatctg gtacagatgg agaagaaaga tggataactc
    10201 ttgaacttaa aatgatagca gatgttggtc ttttaggatt tccaaatgta ggtaaatcaa
    10261 catttctatc agttgtaact aaagctaaac ccaaaattgc aaactatcat tttacaacac
    10321 ttacaccaaa tctaggtgtt gttcaaacta agtttggaga tagttttgta cttgcagata
    10381 tacctggaat tatagagggt gcttctgaag gcataggatt aggtcatgag ttcttaagac
    10441 atgttgagag aacaaaggta ttgatacata ttgtagatat atctggatta gagggaagag
    10501 accctattga agattttgac aagataaatg atgagcttaa attgtacaat gaaaagcttt
    10561 caaagaggcc acaagttgta gttgcaaata aatttgatat attagaagat gagagtaagt
    10621 ttgagaagtt taagagtgaa ttagaaggca gaggatatac agtatttaaa atgtcagcag
    10681 caactcgtca aggcatagat gaagtaatag cctatgtttc taaaatgcta aaagaagtag
    10741 aagatgtgga gctagtttca gaagaagaaa tgtatagacc agaacttgat attggtactg
    10801 aagaagagtt aagtatagat attgaagatg gtgtgtatgt agttacaggt aaagcactta
    10861 gaagaataat gtattctgta aattttgatg atatggaatc ccttcaatat ttccaaaaag
    10921 ctatggagag tcaaggtgtt tttgataggc ttagagaaat gggaatagaa gatggagatg
    10981 tggttaaaat ttatgaatta gaatttgagt tttacaatta atttaaatat agattataga
    11041 taatttaagt atagaattat agaggtgaga atatgttaaa aggaaaacaa agggcgtatt
    11101 taagatcttt agcaaatact ttaaagccta ctacacaaat tggtaaagaa ggtgttacag
    11161 agagtttctt agagcaatta gatggtatgt taagaactag agaattagta aaagttacta
    11221 tattagaaaa tgcaggtctt gatactaaag aaactgctaa tgcagtatgt gaagctttaa
    11281 gagctgaatt tgtgcaggct attggtttta aatttactat ttacaagagg aatattgaag
    11341 agcctaaaat actttttcca ggacatgaac aggcgaaagc aaagactaaa aataataatg
    11401 ttacaaaaaa gggtaaacca actaaaagag ctgttagata atagtttata tccttttagg
    11461 gaaataagtt aaaaaaatag tgacaattgg aagaaattgt attatactat taaacataaa
    11521 ttttaattag caatcttatc aagagtggca gagggactgg ccctgtgaag cccggcaacc
    11581 agtatattta attatacatt ggtgctaaat cctgcagcat ttatgctgaa agatgagtat
    11641 aaacccgtca tatggcttga gtttatactc aagctttttt tattggtata tttatatata
    11701 taaataattt aatataatca aaatgatggg ggtatgttac agtgaagata gcaatattag
    11761 ggtatggaac agtaggaagt ggattaattg atttaataga aaataatact tcaaaaagag
    11821 atatagaagt tgttggaatt ttagttaaaa ataaagaaaa acacaagtat aagaagtatt
    11881 ttgataagat aactactgat atagaagata tatttgacaa ggatattgat atactagttg
    11941 aagtaattgg aggattaaat ccagcttttg attatgtaac aagagcgtta aataaaaaaa
    12001 tacatgtagt gacagctaat aaagatttac tggctgaaaa aggtagtgac cttatagaat
    12061 tagctaactt aaataatgtt agcatcaagt ttgaagcatc tgtggcaggt ggtattccag
    12121 ttttaaaacc attgatagaa tcactggaag gcaacaatat aaagagcata aacgctattt
    12181 taaatgggac ttgtaatttt atattatcaa agatgtatga tgaaaattta ccatatgatg
    12241 ttgctttaaa acaggcacaa gagttgggat ttgcagaagt taatccagat gcagatgttt
    12301 taggatatga tagtgctaga aagttatcta tattgtctac tttatcttat ggaaaaatag
    12361 tatattggaa agatttatta ttagaaggaa taacttcaat agatgaaaaa gatatagaat
    12421 atgcaaaaaa attaaattgt aagataaaat tggttgctag aagtaagtat gaatcaggag
    12481 aagtaagtgg atttgttaga ccagcattag tggacaataa taatatgctt tcaaagatag
    12541 ataatgagtt caacgtagtt attttagtag gtgattcagt aggtgaactt tcttttgtag
    12601 gtaagggagc tggaagaggt gcaacaggaa gtgctgttta tgcagatatt atagatataa
    12661 tagataatag aagttcaaat ataaagtctt tttcaaaagg gaaattggat ttaagtggac
    12721 ttatagaaga tgagtgtagt gcagtagtta gatttggtgg gtataataat aaagatggaa
    12781 ttttagaatg tttagataaa tatgtagata gttatgatat tatagatgat gaagaattgg
    12841 cgatttttgt aagtgcaaag tcagaacatg agattgatag agttttggaa attgtaaaaa
    12901 gaaataatta cagtgaatca gctaaaaaat tattgaagat agattaatat gtttatgcaa
    12961 gaattatgaa aatttaatag ggtgtctcaa aatagagaga caagcctttt ttccagtatt
    13021 gaaataataa aaatatattt gattttataa aaaaagagtg tctcatgaac taaaaagttc
    13081 attttgagac actctttttt aatatctggt gaattaataa gataatagaa aaatgtatac
    13141 gttataatta accaataaag aaattttcac tagcttcatt aatagcttgc tgagttactc
    13201 ttatatatcg ctgtgtagtg gctacagaac tgtgatttaa aagttcttta accaactcta
    13261 aattattgtt actcttttca aattgagtag tagcatagag ctttctaaag ctatgtgtac
    13321 ttatattata cagatttaag tgtttacaaa ttatcctcaa ctgtttttga atagcctttg
    13381 tctttatatt tactaagtag tcatttgatt taagattata ctttcttgca tgttcattta
    13441 tggactcaat tatattttta ttaactggtc tatactgtaa tttgcccgtc tttttttcat
    13501 ttatttccaa catattacct ttaaaattac ctatctttaa tcttaaaata tctgatattc
    13561 ttagacctaa attagcttct aacattagtg ccaatgcaac tttaggattt tttctaaatc
    13621 ttttttcaac gccattttca ctataagtaa agccagtatg taatagttcc attatctttt
    13681 tatattcgtc tatttctata ggtcttgttg ccattttcaa ccactcctct tagtttgatt
    13741 tggtaagttt aggtcggtat ttattatata atataaatga aacattatat aatggaaaaa
    13801 tttcccagta aacttataca agtgtgatat atgtgattaa ggagaatttt ttgaaaaata
    13861 tttctaataa aattattaaa ttgtgatgaa ataactatgc tgttaaacta attaaaaagg
    13921 ttattaaaat tatgacaaat ataataaaaa aatggaagta tttagttaag ttttattagt
    13981 tttatttttt tcgtgaaaat ttcttaataa aaaaagtatt tttatatcgc attagtttcc
    14041 catttgtaga taaagttttc atatgttttt ttgcaataaa tactgctgag attagataaa
    14101 ttattactac aatttgaaga attgtatcat aatagctaag ataataaaac cagattaaat
    14161 tattatatat aacgttgtat gtatcagcaa agctagaaac caacacagca gagtttgcaa
    14221 ttggataaag tataaatata aagatagcag ctaaaactcc atgcgcaaat ttacttagta
    14281 tgtataattt taaatctatg tccgtttttg atagaaatgt acaacattga gctaggatgg
    14341 ataatccact aaatcctatc aaaaagctag ctaaagatgc tcttaaaatt tctggagaac
    14401 ttgttatact agatacttta ttgcatccta tagtaatttc aaatagacca cttacaaatg
    14461 cttgacagag ttctttacta acacctaaaa atgaaagagg caaatatata actgatgcta
    14521 ttaatgagat tacattaaaa agagatagta ttttaaatac tactgaaaaa acaattacaa
    14581 atccaccaac tgtaagtaat gtatttactc cattaaatac agcatttcca aataaaacaa
    14641 aaaaaccatt cccacttgaa tttctaatgt tgattacatt ttttatattt gtttttaaat
    14701 ccaattttgt ctttggaaga ttttcattac cataatttct aaagaaaaga ccaaccaata
    14761 tagttcctag ataatggcag agaatcatta aataacctaa gtttgaattt tggaacatac
    14821 ctgtaccaac cgaacctatt atgaataaag gtcctgatgt agaacaaaaa gacacaagtc
    14881 tttgtgcttc atgcttagaa atttgcccat tactcctaaa ttcagaagct aaacttactc
    14941 ctactggata accagatact gtagatatag caaaagctaa agcgctcttt cctgaaacat
    15001 taaatacaaa tctagttatg ggattgatga taaaccctat aatatccact atttttaaat
    15061 caacaattaa gtttgctcca attataaatg gtagtagtga tggaactaaa acattagacc
    15121 atatagagaa gccttctctt gcagatttta tagcttcatt tgggtatagg ataatcccta
    15181 aaactagaac tccaactata atagctggag caaaaaaagc aataatttta ttttttttca
    15241 taatatcacc tgtaaatttg agttataaga tattatatta aaagtaataa aaatatatga
    15301 atgtaatggt ttaagatata taatagatat atattacaac aacgattatc atattggagg
    15361 aaaaaatgtt taatagtata gctagtaaaa aagtttatga acaagtaatt gagcaaatac
    15421 aatataaaat actaaatgga gagttaaaaa agggggataa attactatca gaaagagaat
    15481 tgtctgaaca aatgaatgtg agtagaactt ccataaggga agctattagg gtattagaga
    15541 ctatgggagt gatagaaagc agacagggtg aaggaaattt tatatgtaca aatatagaaa
    15601 aaactttaat agaaccttta tctatgattt ttaaacttaa taatggcaca cttgaagata
    15661 tattagagct tagaataatc ctagaaattg agattgcaaa acttgcttca aagagaataa
    15721 cttcttctga agttatagaa ttgaaacata taatagatga aatgagagta gaaactaata
    15781 aaaaagataa taatcgagta ctggtgttat tagaccaaaa atttcacagt aaactagcaa
    15841 cattaagtaa aaattattta attcaaagtc tttttatgac agcatcaaaa ttatttgatg
    15901 gatttataga agatgcaaga gaaaaaataa tcgcagaacc ttttaatgaa aatatattgc
    15961 ttaaacagca tgaagctata tacaatgcag ttgtagaaaa tgatgtagaa cttgcatgtg
    16021 agaaagcaaa ggaacatatg gattttataa gtaaaaatta tagaaaaaat gaaaattaaa
    16081 agtttgttat aaaataaatt attcttgcaa atggtagata atggtactat catataaatg
    16141 tgaaactggt atgaccacat accaatttta tagtttatta taatgggaga ttaaaaatgg
    16201 taaagttaaa agtaccgtat tcaaaacaag gaatgatttt agaaatacct gatgaacgtt
    16261 ttctcggtgt tttggagtca aaatcggata attatataga acataagact caagaagaga
    16321 tagttgaaga atctatgaat aatcctatag gctctaattc tctggaagaa ctggttaagg
    16381 ggaaaaagaa tgtagtcata attacaagtg accatacaag acctgtacca agtagaataa
    16441 ctatgccaat tatattaaga aggattagga atgttaatcc agaaattgat gtgaagatta
    16501 tagtggcaac tggatttcat aggccaagta caagggaaga gctaatttat aaaatgggag
    16561 aagaaatagt agataatgaa gatatagtta tgcatatatc tactgatgat aaatctatgt
    16621 gtaaagtagg gatattgcct tcaggtggag atttatatat aaacaaacta gcatatgagg
    16681 cagaactttt gatagcagaa ggttttatag aacctcattt ctttgcaggt ttttcaggtg
    16741 gcagaaaaag cgtacttcca ggaatcgcat ctgcaaagac tataatgtac aatcactgta
    16801 gtgactttat agatagtgat aattcaagaa ctgggaaatt gaataataat cctattcatg
    16861 aggatatggt atatgcagct aaagttgcaa agttagcatt catattgaat gttgtaatag
    16921 ataaagataa aaaaataatt gcttcctttg ctggtgatgt cgaaaaagca catactaaag
    16981 gatgtagatt tgtaacagag ttatcaaaag taaattctat aaaatctgat attgttgtaa
    17041 ctacaaatgg tggatatcca ctagaccaaa atatttatca atctgttaaa ggaatgacag
    17101 cagctgaagc tacttgtaaa gacggtggag taataataat gatttcagca tgtaatgatg
    17161 gtcatggagg tcaatctttt tatgaaaatg tagcaaatgc agattctcca aaacaacttt
    17221 tagataaaat tagaagtgtc gatagagtag atacgattcc agaccaatgg gaatttcaga
    17281 tactagcgag aatattatct aaatatactg tgataatggt aacagataag tgtaatcctg
    17341 aaatgataaa aaaaatgcat atgaaacatg catttaactt taaagaagct ttagaaatgg
    17401 cgaatagtat agttggtaag gagagcaaag tagtagttat acctgatgga gtatcagtta
    17461 tagttaaata aaaaaggagt gtactttatg gaaattttga catgtattaa acaagttcct
    17521 ggaacgactt cagtagaagt tgatgaaact acaggagtac taaaaagaga tggagtagat
    17581 tcaaaaatga atccctatga cctatatgct ttagaaacag ctcttagaat aaaggaagat
    17641 aagaaagcga atctaaaagt acttagcatg ggtcctcctc aggcaaagaa ggtaattgaa
    17701 gaaagtttta tgatgggagc tgatgaagga gcattaataa gtgataggag atttggagga
    17761 gcagatgtat tggctacttc ctataccata tcacaaggta ttaagaagat gggaaaggta
    17821 gacctaataa tttgtggaaa acaaactact gatggtgata cagcacaggt gggacctgaa
    17881 gtagctgaat ttttagatat acctcatgtt actaacgtta caaaattaat tgaagttaaa
    17941 gatgaaagta tagttgttga aatagatatg ccaaatgatt tacaagtatg tgagattgaa
    18001 tatccatgtt taataactgt agaaaaagac atttttcaac caagactacc ttcatttaaa
    18061 ttaaaactta atactaaaga tagagaaata cctgtatata gcttagatga ctttgaagat
    18121 aaagatgaaa ataactatgg acttaacggt tctcctactc aagttgtaag aatatttcct
    18181 ccaaagccaa atacagataa aaatattgta agaggaaatg cagatgaact aagttttgca
    18241 cttgtaaata aattagagga attaaagttg gtgtaagggg gagtaataaa tgtcaaagat
    18301 agttgtaaat caagataaaa taactgactt aaagagaatt ttagaaatat gtccttttgg
    18361 ggctatagaa gaaaagtcag gtatagtaga aataagtgct ggctgtaaga tgtgcaagtt
    18421 gtgtgtcaaa agtggaccaa aaggcgcatt tgaatttata gagagtagta aagtacaaat
    18481 taataaagat gaatggaggg gaattgcagt atatgttgaa catcataatg gaaatataca
    18541 tcctgtaacg tacgagctta taggtaaagc tcgtgagatg gcatcaaaaa taaaacaacc
    18601 tgtgtattgt gtatttgtag gaaaagacat aaaagataag tgcagtaatt tgatttctta
    18661 tggggtggat gaagtatttg tatatgatga agatgaattt aaagatttta ggatagaacc
    18721 atattcaaaa gcaatagaaa actttataga taaaataaaa ccaacgatag tattagttgg
    18781 tggaactaca ttaggtaggt cattagctcc aagacttgcc gctagattta gaacaggtct
    18841 tacagcagat tgtacaatac ttgatattca atcaaatact gatttagacc aaataagacc
    18901 agcatttggt ggaaatataa tggctcatat aaacacacct aataatagac cacaatttgc
    18961 cactgtaaga tataaaatat tttcagcacc agaaaaaata gagaatacta caggaaagat
    19021 aactttatgt aaagtagaaa aaaaagaact aaaatctaaa attaaggtac tgagtgtaaa
    19081 agaaaaaaat aaagaagttg gtctggaaga agctgaagtg atagttgtgg caagcagagc
    19141 aataaaaaaa caagaagata tggagatgat atataaatta gctgataagc taaacgctca
    19201 agtggcagga acaagacctg taatagaagc aggttggata gatgctaaaa aacaaatagg
    19261 tcttagtggt agaactgtaa aacctaaatt gataataact tgtggtgtat ctggagcagt
    19321 tcaatttgtt gcagggatgc agggtgctga ctatatagta gcaataaata aagatgataa
    19381 agcgcctata cttgatgtag cacatttagc tttaataggt gatatttatg atattattcc
    19441 taaattgatt gaaaaaatag aaaataacaa agataataat caaaaatata tggcatcagc
    19501 agctaaatag ggggatagaa atgtataaat taatcgataa aaaagatata gattttttaa
    19561 tagatacttg tggagaagaa aatgtattag ttggaagtga tataaatgaa gatttttcac
    19621 atgatgaact tggagggatt gagaagtacc ctgaagtttt agtaaatgta cttgaaactg
    19681 aacaagtatc taaaataatg aaatacgcat ataaaaataa tataccagtc acaccaagag
    19741 gtcaaggtac tggtcttgta ggtgctgctg ttgccataaa tggtggaata atgattaatc
    19801 tatgtaaaat gaataaaata cttgaagttg attatgaaaa tttaacattg acagtagaac
    19861 caggtgtttt attgatgaca ataggtcaat atgttcaaga tagagattta ttttacccac
    19921 ctgacccagg tgaaaaaagt gctacaattg caggtaatat aaacactaat gcaggtggta
    19981 tgagagcagt aaaatatggc gttacaaggg attatgtaag aggacttgaa gttgtactac
    20041 caaatggaga aattataaat gtaggtggaa aggtggttaa aaatagttct ggatatagta
    20101 taaaggattt attagtaggt tcagaaggta ctttaggaat agttactaaa gctatattaa
    20161 aattgcttcc tttaccaaag aaaagtataa gtttgcttat accatttcca gatttatcaa
    20221 tggcaataga aactgttcct aaaataataa aatcaaaatc aataccaacg gctatagaat
    20281 ttatggaaag agatgtaata ttggcagcag aagaattttt aggtaaaaag tttccagata
    20341 atacatcaga tgcttatctt ttacttacat ttgatggaaa tagcactgaa gatatagaaa
    20401 aggaatatga aaaagtagct aatttatgtt tagaaaatgg tgcattagat gtatttatat
    20461 cagatacaca agaaagaaac gattctatat ggtcagccag aggtgcattt ttagaagcta
    20521 taaaggcatc tacgacacaa atggatgaat gtgatgtcgt tgttccaaga gataagatag
    20581 cagagtttat aagatacact cacgagcttc aagacaaatt aaaaataaga ataaaaagtt
    20641 ttggtcatgc aggagatgga aatctacaca tatatatttt aaaagatggt atggatgata
    20701 atacatggaa aataagatta aaagaaactt ttgactatat gtataaaaaa tctagagaac
    20761 ttagtggtca agtttctggt gaacatggca taggatatgc taaaaaagag tacttacatg
    20821 aatcaaattc agatgcttat atgatgttaa ttaaaaatat aaagttagct tttgacccta
    20881 aaaatatttt aaatccaggg aaaatatatt agaaatatta atttaataat atttacaaaa
    20941 caaaaaatat taaattagat ttaaatataa tattttaaac tttactttta aatctgaagc
    21001 taaattgtaa aatttttagg atggcaatat ttacttaaat ttagaaagta acataatatt
    21061 taagttgatg ttgtcatcct aaaagcatgt gtacataaat aaagtaagac tattgtatct
    21121 tatataataa agagttaata aaatgatttt tataaatata gttattttaa aaaactactt
    21181 tacatatatt ggatgagtat aaaaatccat agggcctttt actaaatctt tattgttttt
    21241 ataatatatt gaattatata tatttgtagc ttttatatca taacttatga gtgttttaaa
    21301 aatcgtatca tctaatgaaa atgatatatt tgaaaacttg tttattattt ttatttcaga
    21361 tttatttttt atctctttta gaatttctct acctttatca ttaaaagcaa gtattcttat
    21421 ataaggtata gtatctatgt tttttataag ttgcatgtcg ctttttttaa tacctaacaa
    21481 tatattgttt agagttcgtt tgattttggt taaagtgtat cgcttggatt taattgctaa
    21541 ttgtagctca tgtagaaatt cagatgtaaa gatatcttta tagattctgt tttctatgcc
    21601 ttcatttact tcaaaatact cttttaaagt gtccatatct ctaagtataa ttgtttttaa
    21661 aatatcaaag aagaagtcat caaacatagg taaaaaacca ttatctactt tttcatttag
    21721 gatgttataa gttttttctg gaataacatt ttttaaatga aaaaggtctg tattttcttt
    21781 aagtgaagtc cttatagcag tagcagaaca aatatcactc tcaatttctt cggaattata
    21841 ttctgattgg accctggaga tagtaaatgg ttttatacta ctgtttaaat ggatgaggtt
    21901 ttttatgtat tctataccca gtatgttgtt tgatgaattt aatgtttttg ataattgttc
    21961 ctttgaataa ttttgtataa aattattttt tttgtcttga tgtatataat caaaaagtgc
    22021 attagctcta gctgttggaa ataacaggcc atcatctaaa tattttttta aaagtaactt
    22081 aaattcattt ggttcatttg ctaaaatttc agctatatta taaagaattt ctgtatttcc
    22141 ttcttcacta ccaaaacata tagaatttat gcagtttagt gaatttaaaa ttgttatagc
    22201 accatgagaa aaaatttcag cactttgaca agcaaataaa gttggtaatt ctataactaa
    22261 gtctacacca ttttcgacag caatttttgc tctagtatat ttatcaaata aagctggttc
    22321 gcctctttgt agaaaatttc cactcattat tgctacagta tgtgttgcat ttgttttttc
    22381 tatagattta gataaatgat atatgtgtcc attatgaaat gggttatatt caactattaa
    22441 tccaagtatg ttcataaaat actcctttta tctttaatta ttttatatat atcacaattt
    22501 attataatat gtataaaatt taaattttta attataaaat taaaaaacgt aaaaaaattt
    22561 ataaaaatgt agaaatgtta cctacataaa aaactggtta aatgttatta aaataacttt
    22621 attagctctt tatattatat tattataaca aatgggaaag aataatctac attaagttaa
    22681 cttgttaata tattattagt gaaatacaca atttaccata ctagaaatat gatataatat
    22741 aaaaaaaaag tatatacttt tatattaaaa ggtgatatat tatttatgaa aatcgtttaa
    22801 tgtatacaat aatatatcta taacaaaata ataaaagata gagttttatt tagacattta
    22861 ttacaaatca aaatttatta catattattt ataaaaaaat ctaaggagga aataataatg
    22921 aaaatattag tattaaactg tgggagttct tcattaaaat atcaattaat agatatgaat
    22981 aatgaagaag ttttatgtat aggattggtt gaaagaatag gaatagaagg ttctatatta
    23041 aaacatgaaa aagcaggtag agatgataaa tacgttgttg aacaaccaat gaaggaccac
    23101 aaagatgcaa tagcattagt tttagaagct gttgctcatc cagaatttgg tgcagttaaa
    23161 gaaatgaaag aaatagatgc agtaggacat agagttgtac atgctggaga aaaatttgca
    23221 acttcagtag taataactcc agaagtagaa gaagctttaa aagaatgtat agatttagct
    23281 ccacttcata atccagcaaa cataatggga atagatgctt gtaaagctat acttccagat
    23341 gtaccaatgg taggtgtatt tgatactgct ttccatcaaa caatgcctaa atcttcatac
    23401 ttatatggtt tacctcatga gctatatact aaatatgggg taagaagata tggattccac
    23461 ggaacttctc ataattatgt atctcagaga gcagcagaga tattaggaaa agatataaaa
    23521 gatttaaaaa tagtaacttg tcacttagga aatggtgctt ctatagctgc tgttgatggt
    23581 ggaaaatgtg tagatacatc tatgggattc actccattag aaggattaat aatgggaact
    23641 agatgtggag atatagaccc agctatatta ccattcttaa tgagaaaaga aggtttagat
    23701 gctgatggat tggataaatt aatgaataaa gaatctggag tatatggaat gactggaatt
    23761 tctagtgact tcagagatat agaagatgca gctaaaaatg gagacgaaag agctcaggct
    23821 acattagaag cgtatgttaa aaaagtacaa aaatatatag gcgcttatgc tgctgaaatg
    23881 aatggattgg atgttgtagt atttactgca ggcgttggtg aaaatggaaa ggctataaga
    23941 gcagatattg cttctaacat ggagttttta ggaatgaaat tagataaaga agctaatgat
    24001 gttagaggaa aagaaacagt aatatctact gctgattcta aagtaaaaat gcttttaata
    24061 ccaactaatg aagaattaat gatagctaga gatacattaa gattggtaaa ataattaaat
    24121 taaacctatg cctatcaagt aaaaatactt gacaaacaaa tgctagattt gtataataaa
    24181 tttggtgata tgttgaggtg aacttaatga aagttagcat agaaaaaata aatagaaaag
    24241 aaactgacaa aatagacttg aatttttgtg aaaaaattga tactattagt tattgtgatg
    24301 agatttacaa attagtatca cctgtaaatt taaaagggaa agtgtcaaag actaataaag
    24361 gtttgtatct agatatagat gtcaatttta caattgttga cacttgttca agatgtctta
    24421 aagaagtaga aataccacta gagtattcta tacaaggttt tttagtaaaa gaagaagatt
    24481 atgatgaaga tgagtttgag gaatttgatc cttttatatt cgatggtgaa gaaatcgacc
    24541 ttattgatat aatagaacag acattagatt ttaatgtacc tcataaagtt ctttgtagtg
    24601 aaaactgtaa aggtctttgt caagtatgcg gggcaaactt aaatgaagaa gagtgttctt
    24661 gcagtgaaat tacaaacgat gaggaataca tagatcctcg ttttgctaaa ttaaaagatt
    24721 tatttaatta aattttgact aaggaggtgg cgtaaatggc agtaccaaag cgtaaaacgt
    24781 ctaaatcaaa cacaaaaatg agaagagcag ctaactcaaa gatggaagca acaggatttg
    24841 taagttgccc acaatgtcat gagccaaaat taccacatag agtgtgtcca gactgtggat
    24901 attataaagg taaagaagtt gtgtctaagt aatagcttct aaaaagcatg tagagtatac
    24961 tctacatgtt ttttttgtac atataaaaaa ataagatgat ttactagaga aaataagtgg
    25021 attttatgca tattaaaaaa tagaaaaata ataatataag tattgaaaat ttttgtgtgt
    25081 tactatatac tactattagt agctggtact aattacagct ataaaatttg gagggaacat
    25141 gaaaaagaaa agtaaagccc aaagacaaaa agagcttata gatatgctaa agacagatcc
    25201 tttttatact gatgaagaat tatcaagtct gtttgatgta agtatacaga ccataagatt
    25261 agatagaatg agtctaaaca taccagagct aagagaaaga gtcaaatcaa tagcagaaac
    25321 tcagagttca aaagtaaaga ctttaggagt aaaggaaatt acaggagaaa tcattgattt
    25381 atctgtaggg cgattaggta tatctatgtt agaagttaca caggacatga tttactctaa
    25441 gactaatact ttaaaagata cgtatatttt ttctctagca gattcattgg caatggctat
    25501 aatagatgca cctaaggtta ttatgagagt tgcaaatgta aaaagtttta agttgataga
    25561 acaacaagat agacttatag caaaagcaga agtgtataga aatatagata aaaagcatta
    25621 tgtaaaagtt gttatcaaca ataaagccca agagcaaata tttagaggaa agtttatctt
    25681 tgaagaatta gattaggagg gaaattatga aaatagtaat tgatggaatg ggtggagata
    25741 atgcacctaa atctaatgta gaaggcgcag taaatgctat aaaagaatat caggttgatt
    25801 taataatcac aggagataaa gatttacttg aaaaggaatt ttcaaattat gaatttgata
    25861 gaaataaatt agagatagtt catactactg agattataga aaacgaagat aaaccagtaa
    25921 aggctataag aagtaaaaaa gattcatcta tggtagtagc attaaatctt gtaaaagaag
    25981 gaaaggcaga tgctataata tcagctggaa atacaggagc cttacttgct ggtggattat
    26041 ttgttgttgg tagaataaaa ggaatcgata gaccttgctt atgttcagca ataccaaatg
    26101 taaagcgagg aatgacactt atagcagact gtggcgcaaa tgcagattgt aaacctaaga
    26161 atctagttga atttgcagct atgagtaata tttattcaag aaaagtttta ggtcttgaaa
    26221 atcctaaggt agcattagca aatgttggac tagaagaagg caaaggcaat gatttagtca
    26281 aaaggtcata tgaagaaatt aaaaaattag atttaaattt tatagggaat gtagaagcta
    26341 gagaagttat aaatgcatac acagatataa tcatatgtga tggatttaca ggtaatatat
    26401 tacttaaatc tgctgaaggt gtagcacttt cagttatgag tcttataaag gaaactttta
    26461 tggccagtac aaaaagtaaa ataggagcat tacttataaa ggatgattta agaaaattaa
    26521 aaagttttat agattattct gagtatgggg gagctccact tttaggtcta aatggaggag
    26581 ttataaaagc tcatggtagt tctgatgcta aagctataaa aaatgctata aatcaaggaa
    26641 taaaattttc aaagggaaaa gtcgttgaag atattaacca atttataagc aaatacaatg
    26701 aagaaaataa aaataacgaa gatgaataaa ctcgttttgg aggtttaata tataatgaat
    26761 acaaaggctg gaatattagg agtaggaagt tatctacctg agcaatcata tgataatttt
    26821 cacttcgaaa aaataatgga tacatcagat gagtggataa gtactagaac tggtattaag
    26881 gaaagaagat ttgccaaaga gtctgaagca acttctgact tagcatctaa agctgcctta
    26941 aaagctatag aatgtgcaaa gttaaacgta gaggatatag agctgattat attagctaca
    27001 attactcctg atatgtcttt gccttcaact gcatgtatag tacaagatgc aataggagca
    27061 gtgaatgcta ctgcatttga tatatcagca gcttgttctg gatttgtata tggagtaact
    27121 attgcaaagc aatttgtgga aacaggttgc tataaaaatg tgcttgtaat aggagcagaa
    27181 acttgttcta agtttttaaa ttatgatgat agaacaacag cagtattgtt tggagatgga
    27241 gctggagcag ctgttattgg tcctgtaaat gagggtggaa tactgtctac acatatgggc
    27301 tctgatggta aaggaaaaga ttgtttaaaa gtacctgctg gaggttcaag acttaaggca
    27361 agtaaagaga ctgtagaagc taatctacat acaatagaaa tggcaggtag tgatgtgttt
    27421 aaatttgcag ttagaaagat ggcagaaact tctttaagag ctcttgaaaa agcaaaccta
    27481 aatacaactg atatagacta cttagtgcca catcaagcta atataagaat catacaagct
    27541 tcttctaaga gattagagct tgatatgaaa aaagtttatg taaatataga caaatatgga
    27601 aatatgtctg ctgcatctat accagtagca ttagatgaag cttatagaga aggtaaaatt
    27661 aaaaagggtg ataatgtagt tttagttggc tttggtggag gtctaacttg gggggcttca
    27721 gttgtcaagt ggactttata gtataaaata tctaaaatga tttaattaac aaaattaaac
    27781 ttatacacaa aataaagttg attaataata aaaagtttac ttttatgatt tcaagtgaag
    27841 gagaattgta tgaataaaat ttgcaaaata ttaaatataa aatacccagt tatccaaggg
    27901 ggaatggcat gggtagctac tgcatcatta gcaagtgctg tatctaatgc aggaggactt
    27961 ggcataatag cagcaggaaa cgcaccaaaa gaagctataa agaaagaaat tgttgagtgt
    28021 aaaaaattaa cagataaacc ttttggagta aatgtaatgc ttatgtcgcc atttgttgat
    28081 gatataattg atttgattat agaagaaaaa gttcaagtta ttactactgg tgctggaaat
    28141 cctgcaaaat atatggatag attaaaggaa gctggaacaa aggttattcc tgtagtacct
    28201 acaatagctt tggcacaaag aatggaaaag ttaggagcta cagcagtaat agcagaaggt
    28261 actgaaggtg gaggacatat aggagaactt actactatgg tcttagttcc acaagttgct
    28321 gatgctgtaa acatacctgt aatagctgct ggaggaattg tagatggtag aggaattgca
    28381 gcatcatttg cattaggtgc cagtgcagtt caagtaggaa ctagatttat ttgcagtgaa
    28441 gagtgttctg tgcattcaaa ctataaaaac ttagtactaa aagcaaaaga tagagatgca
    28501 attgtaacag gaagaagtac tggtcatcca gtaagaacat taaaaaataa actatcaaaa
    28561 gaatttttaa agatggaaca aaatggagct actcctgaag aattggataa aaaaggtaca
    28621 ggagctttaa gatttgcaac agtagatgga gacatagaaa aaggttcatt tatggcaggt
    28681 caaagtgctg ctatggtaaa agaaataaca ccttgtaagg aaattataga ggctatggta
    28741 aatcaagcaa gagagattat gccagcaata gaactgtaaa tcaggaggag caagatggga
    28801 aaagttgcac tagtattccc tggtcaagga gctcaatatg taggtatggc caaggattta
    28861 tatgaaaata atgctgttgc taaaagtgta atagatgagg ctagtgatgc cttaaatatg
    28921 gatttaaaaa accttatgtt taatggaaat gaagaagaac tttctaaaac tgaaaacact
    28981 caacctgcta tggttacaca tagtgtagca gtattaaaag cggtacaagc tcaaatagat
    29041 ttaaaatata atgcatgctt aggattatcc ttaggagagt atagtgcgtt ggttgcagca
    29101 gatgcaattg actttaaaga tgctgtatgt ttagttaaaa aaagaggtaa gtttatgcag
    29161 gaaacagttc cagcaggtgt tggagctatg gcagcaattt taggtttaga tagaaatgta
    29221 cttgaaaatg tggttaaaga tatacaagga ggtattgtag aagtagcaaa ttacaattct
    29281 cctggacaaa tagttatatc tggtgaaaat gaaaagatag aagaagcaat ggtaaaatgc
    29341 aaagaagcag gagcaaaaag ggcagttaaa ttaaatgtaa gtggaccttt tcactcatca
    29401 atgttaaaag aagctggtgt aaaattggcc agtgaattag aaaaagttaa cataactaaa
    29461 cctaaagttg atgtggtggc aaatgtaaat gcagattact ataaaaacga aggtaaagac
    29521 ctgcttataa aacaagtaag ttcatctgtt ctttgggaag attctataga aatactttta
    29581 aatgatggat atgatacttt tatagaaatg ggacctggaa aaactttaaa agcatttatt
    29641 aagaagatag cttccaataa aaaagcaaat gtcaatattt ataatataga tggtatagat
    29701 tctcttaatg aatttgtaca aaattataga aatggagaga tataatgata aatcttacag
    29761 gacaagtagc agttgttact ggtggctcta ggggtatagg aaaagaaata gcaaaaaaac
    29821 tagcatcttt tggagctgat gtagtaatca attatacttc taaagaagat gaagcactaa
    29881 aaactaaaaa tgaaatagaa agtatggggg taaagtgtac ctctataaaa tgtgatgtgt
    29941 ctaaatttga tgaagtgaat caaatgatag attctgttgt aagcgaattt ggaaaaattg
    30001 atatattggt taataatgca ggcataacta aagatggtct gcttatgaga atgaaagaag
    30061 aagattttga tagagttata gatataaact taaaaggtgt ctttaattgt acaaaagcag
    30121 ttactaaacc tatgatgaaa aagaagtatg gaagaataat aaatatgact tcagtagttg
    30181 gaattatggg taatgcaggg caaactaatt attgtgcatc aaaagcaggt gtaattggat
    30241 ttacaaaagc ttctgcaaga gagttagcat caagaaacat aaatataaat gcagtagcac
    30301 ctggatttat agaaacagat atgactagag tactaagtga tgatgtaaaa gaatcaacac
    30361 tagcaaacat accaaagaaa tcttatggta aaccagaaga tgtagccaat gccgtagcat
    30421 ttttagttag tgacatgtca agttatataa caggacaagt aataaatgta gatggtggaa
    30481 tggtaatgca ataataattt aaaaagtaga gaaatctatt aaaataaaaa attttaggag
    30541 gtacacaatg tttaataaag ttgtagaaat aataatagag caattaggag tagaagataa
    30601 agaaataact atggagacat cattaatgaa ggatttagaa gcagattcat tagatgcagt
    30661 agaaataata atggcacttg aagatgaatt tggtatagaa ataccagata cagaagcaga
    30721 aaacttcaaa tctataggag atatagttaa gtatatagaa gctaataaat agtcgagctt
    30781 taaaaaagtg gaggtatccg atgaataaaa gagtagtaat aacaggactt ggatgtgtta
    30841 cgccgttggg aacgggaaaa gaagagttct ggagtaatat aaaatctgga gtatctggta
    30901 tagataaaat aactaatttt gatgctagca catatcaaac tcagatagca ggagaagtta
    30961 agaattttca tccagaagaa tatataagta aaaaagaatt gaagagatta gataaatttg
    31021 ctcaatttgc aatagtatct gctaaattag cagttgaaga tgcaaatctt gatttagata
    31081 aagttgatag agaacgtttt ggtgttataa taggttctgg aataggtgga gttgaagcta
    31141 tagaaactca acataaaatt cttttggaaa aaggaaataa aagagtaagt tcactttttg
    31201 tacctatgat gataggaaat atggcagctg gtcaagtttc aatattttta ggagcaaaag
    31261 ggcctaacac taatgtatgt acagcttgtg catcaggaac tcattcaata ggtgatgctt
    31321 ttaaggtaat tcaaagaggt gatgcagaca tcatggtagc agggggctct gaagctgctg
    31381 taacaggtct tgcttttgct ggattctgta acatgaaggc tatgtcaact agaaatgatg
    31441 accctaaaac tgcatcaaga ccgtttgata aagacagaga tggttttgta atgggagaag
    31501 gagcaggtat tgttatctta gaagacttag aacatgcttt agcaagaggg gctaaaatat
    31561 atgcagaagt agttggatat ggtttaactg ctgatgcata ccatatgaca actccagctg
    31621 aaaatggaga gggagcagct aggtctatga atatggcttt gaaagatgga aatgttccat
    31681 tagaagaagt agattatata aatgctcatg gaacttcaac ttattacaat gatttatatg
    31741 aaactatggc tataaaaact gtatttggag aaaaggcata tgacttatgt gtgtcatcaa
    31801 caaaatctat gactggtcac ttattaggag catcaggtgc tatagaggct gttgtttgtg
    31861 ctatgagtat agaagatagt tttgtgcctc caactataaa catacaagaa gttggtgaag
    31921 atttggattt agattatgtt ccaaatcaag gtaaagaaaa aaatattaga tatgcactat
    31981 caaattcttt aggatttggt gggcataatg ctacaatagt actaaaaaaa tatgtataaa
    32041 taaatattaa atataaaact aaaaaacaag cacttgtttt aactgtataa atttatatta
    32101 gcttaaattt aaagtctcct aaattatttt aggagacttt tttattaaga tttaagtcta
    32161 gcatatgttg taaatcattt tgatatattg tattattaat tattaagagg ttatttatat
    32221 atgcaagttt tcattctaca atatacaaaa ggaggactaa aattttgaat tttagactac
    32281 caaagaaaag gttacccaaa aagataattt ttatatgtat aatacttatt agtattattt
    32341 tgtttataaa gtatgaccaa aatggattga aagaaagaga aattataaat aagggaaaat
    32401 atatgtctga aagccttgtg gcagataagg agtatgatga agcaaaaaaa gtggttaaga
    32461 cttctttcga atctattcca gaaaaaaaat acaataaagt atataatgaa gtttgggata
    32521 tgttaaagac tatttcatat gttccagatg gaacgaagat agtcattgat tctttagaaa
    32581 agatgagaga atcagatgtg aaattatctg atgaatgtag atttaatata gaaaaaagat
    32641 tagcatcagt gtatataatg gataataatt attctaaggc agtagattta actgtaaagt
    32701 ctataaaatt ggctgagaaa ttggggaata attatgaaaa agcaagacta gatgttgatt
    32761 tggcgagcat atttttaaat ataggtagct atgaaacagg agagaaatta ataaaggatt
    32821 cttttaaaat agatattgat gaaggtgaaa aaaatggaat ggttagatta tatgctatta
    32881 taaatctagc agaaatatat gtagaagagg gagaatatga taaagctatt gaaataagta
    32941 gcaggataaa agattataaa aagtttgtaa atacggataa ttacaatgat tttgatataa
    33001 tggcctctat aatgaagtca aatgcgtatg tgggcaaaaa taatatacaa aaggcaaaga
    33061 cttttttaga aaaagctgat tatcttataa aatcagatag aacagtttat atattagata
    33121 aagatatgta ctattatatg gcaatgggaa acttagaata taaaagcgaa aattataatt
    33181 ctgcaataga taattataaa aaaagtttag agatatctac caaaagaaag ttaactcagg
    33241 agaaaataaa aaatttaaat aagatattaa atatatatga aaaacaagga aatatagaat
    33301 ctttaaatga gtatcaaaag atgttaataa atgaatataa agagaacaaa agtataagag
    33361 attcagagac tagtttctat ataattgata aagtatcaaa cgaaagtaag ctatttgaaa
    33421 aaactaaaaa agaaataaaa tattataaaa tattatttac agtaatttta attgctatat
    33481 tagggtctac ttttttatac aaccgattga agtattttaa agcacaaaat ttacatgatg
    33541 gtcttacaaa tgtttataat agaaaaagtt ttgatattat gtatgaaaag tacagagaga
    33601 aagatgataa ttttgcactt gtgatgatag atattgataa tttcaagctc ataaatgata
    33661 attatggaca taaatttggt gacactgtta taaagggaat aacaaatacc atttgtgtta
    33721 tgttagaaca agatgataaa gtctttaggt acggaggaga agaattttct gtattaataa
    33781 gaaataaatc tggtgaggaa gtagcagata taattgacaa tataagggtt gcaattgcaa
    33841 ataaaaagtg gaatgatgat gtgactgtga ctataagtgc aggtgtagct catatttcag
    33901 atagcaaaga tgtactagaa gaagctgata aaaatttata taaagcaaaa caacttggta
    33961 gaaataaagt agtatataag taattatata ctatataaat aagaggtata aatatgaaac
    34021 aatcaatagc tatagtaact gataaaataa ctaaactatc atctttctta gaagaaaata
    34081 tacgtttggt tttaggtaat tatattgata taaaccacta ttatctggaa gttttaaaag
    34141 agaatgataa aatacaaggt gattttgtac tagtaatgaa tgatgataga ttaaacagta
    34201 tgagaaagca tttagtacaa gatagtaaga ttatagtgat aaggagaaca cttaaagaaa
    34261 atgaaatata tggattattt tcaataccaa atggaactga agtattggtt gtaaatgata
    34321 caaaagaaac taccttagaa acgataagct tattttacaa aataggagta aaaaatttaa
    34381 agtttactcc atatattgaa ggaaaaattt ataaaaatat agacatagct ataacgcctg
    34441 gagtaaagga aaaagtacca agttatataa ataaagtaat cgaccttggt aatagatata
    34501 ttgatatatc tacttttata gaaataataa atggtcttag aattgatgtt aaagaaatac
    34561 gtaaaaattt aatggcatat tctgagagtt taataagttt agataatgga gttaaagata
    34621 attataggca actgttttta aaaatagaag aacaagatgt tatattgaat ctatcaaaag
    34681 atggaataat atttacttca gcagatggta taataaacac atttaataaa gaagctttaa
    34741 aaattttagg tataaatgaa aatccacaag gcaaaaacat agaagatatc atttctgaag
    34801 atttaaatat tttacttgaa gacaaagaaa taattgatga agtggtattt acaaataaaa
    34861 aatatataaa tgtaaataaa aaaagtattt tttctatggg gaataaagct gggatatatt
    34921 ataacttaca agaaataacg tatataaaaa aattagaaca aaatcttact aataaattaa
    34981 gagaacaagg tcagatagca agatatacat ttaaagatat aaaaactaaa aataaaaata
    35041 tggtaaagtg tgtagaattg gctaaaaaaa tatctaaatc agacctttct gtattaataa
    35101 taggagaaag tggtactggt aaagaattat tatcacaatc aattcataat gcttccaata
    35161 gaagtaatca accatttata gcggttaatt gtgcagcagt tccagatagt ctattagaaa
    35221 gtcaactgtt tggatatgaa aaaggctctt ttacaggagc tttaagagaa ggaaaaaagg
    35281 gtctatttga acttgctaat aatggaacta tatttctgga tgaaattgga gatatgcctc
    35341 cattgctaca gacaaagtta ctaagggtat tacaagaaaa gcaggttatg cctataggtt
    35401 cccataatgt tataaacata gatgttaggg tcatagcagc aacaaacaaa aacttactaa
    35461 agatgattag agaaggaaaa tttagagagg acctgtatta tagactaaat gtattgccaa
    35521 tagatgtacc atcattgagg aatagaaaag aagatataat aacacttatg aatttctttt
    35581 tgaaaaacaa tgtaagactt tcgccagaag ttataactat tttagaaaaa tataattggc
    35641 caggaaatat aagagagtta caaaatgttg cttcttatgt aagtctaatg tgtgatagta
    35701 tggtttttag agatgactta ccaccatata taagggtaga atgtgctaac aattatgaag
    35761 atggatttgg ttatacaaaa aaagaatttg aggaaatact gaggattcta tatgaaagaa
    35821 gagagtcatc ctctggagtt ggaagagggt ttataatcga aaagcttata caggaaggga
    35881 ttgagatttc tgaaagcaaa gttagaaagg tgctatctta tttaagtgaa tgtggatata
    35941 tatcatctaa atctggtaga ggaggaagtc aaataacctc agaggggata aaactttata
    36001 ataatataaa ttaagctaag tatttgatat tgaaatcaca tattttgaat ttagatattt
    36061 aaataataaa cttttatatt tatagagatt aatttatact atgtcagaaa gaatattgta
    36121 atttataatt aaatattaat aggtttttaa aataggatta gataggtaat aggttaagtt
    36181 agaataaccc attacctatt tttatttgta agaaaagtgt acatagagtt aaaagtaaaa
    36241 aatacaacta ataaaactat tgaaaacact agataaataa taaataaaaa taattataaa
    36301 ttttggcata tatattgcta atcatagaga taagacgaca aaataattgg gaggtttaat
    36361 gatgaaatac aaaacaacag ggacttgtgc tacagaaatt gaatttgaag taaaagaaaa
    36421 taaagttaca aatgtaaact ttataggtgg atgtgatgga aatttaaaag ggctaaaagt
    36481 attagttgaa ggcatgaata tagaagatgt aatacaaaag ttaaaaggaa tagagtgtaa
    36541 aacaaagcca acatcttgcc cagaccaact gtctctagca ttggaaaatt atatgaatat
    36601 aaagtaatac ataaaacaat cgattagtta ggagtttaat tatgaaacca ataataggga
    36661 ttttaggaaa tttaataata atggaaaatg gtatgtttcc aggtcttgaa aggtcatatg
    36721 taaataatga ttatataaat gcagtgctaa aaggtggcgg tagtccagta ataattcctg
    36781 taaatacaga taaagaagtt ataaaaaaac agattgaaat ggttgatgga gtcttaatat
    36841 ctgggggatg ggatatcaat cctcaactgt atggagaaga aacaagagaa gaaacgacat
    36901 ttatatatcc agaagtggat gaatttgatt taattgcaat aagtatagca ttagaactta
    36961 aaaagcctat attaggtgta tgtaggggac tacaaatttt aaatgtaagt ttaggaggaa
    37021 ctttatatca agataataat cttatagaag gaagttatat aaaacataca caatcttcta
    37081 aaagacatgt agctactcat aaagtcgatg ttaaagaagg aagtatttta gagggaattt
    37141 taggaaaaca attacttaca aatagttatc atcatcaaag tgtcaatcag cttggaaaag
    37201 gattaaaagc catagcatac tcaaaagatg gtataataga agctattgag aaagaggagg
    37261 aaaattttgt agtaggagta cagtggcatc cagaaatgat ggtagattac tgtgataaaa
    37321 tggaaaaact atttaaatat tttataaata tttgttcaaa tttataatat accttggggg
    37381 gattgaacat gaacgaaaag aacaaaatgg gattaataag tattatactt ttaggtatta
    37441 atgcagtagt aggagctgga gtttttctat taccaggaga tgcaatgaaa tcatttggtg
    37501 tagcaagtat atttgtatat atatttgata tgttgttagt tttatctatg gcattctgtt
    37561 ttgctgaggt tgcaggtaaa ttcaataaaa atggcgctgc ctatgtatat accaaagaag
    37621 cttttggaga tttttgtggt tttgaagtag gtcttatgaa atgggtaata ggatgtattt
    37681 cttggggagc tttaatagta ggttttccta catcattatc agcagtatgg gctccagcag
    37741 gtgaaccaca tatacaaaaa attataattg tagccatgat agttgggctt acaattataa
    37801 acttattagg tgtatctcta tcaaaaattg tacaaaatgt tataactgta ggtaaactaa
    37861 ttccattaat actatttata ggaataggta tattttttat aaaaggagtt aattttactt
    37921 cttcaacaat ggtacctcca ggagctggag caacagagtt tggagcagca gcacttttaa
    37981 tgttttattc atttacagga tttgaatcta tagcagttgc agcagaagat atggaaaatc
    38041 cacaaaaaaa tataccaata gcaattataa gtgtaatagt aattgcatca ataatatata
    38101 tattaaatca agttgtatgt gttggtatac taggagattc gttatctagt acatctacac
    38161 cagtggcaga tgctgcaaga atatgctttg gaaatatggg agctggattg gttacctttg
    38221 gaacattggt ttctgttggt ggaatttgta tgtgtggtgc ttttgtaaat ccaagaagtt
    38281 gtgtagcttt agctgatgat aaaatgttac caagaatatt tgcaagaaaa gataaaaaag
    38341 gtacaccata tgtagcgata atagctacta tgttaatcac tataccaata gctctatctg
    38401 gaagttttgc tgaattagca gcaataagtg cagttgctag attcatacaa tacataccaa
    38461 catctttatc cgtattagtg tttagaaaga aaagaccaga attagtaggt acttttaaaa
    38521 ctccttttgg agcagtgatt cctctaattg ctgtatgtgt tggaacatgg ttgttattcc
    38581 aagcatctat gcatcaatta attatgggat taggagcttt ggcaattgga gtaccattat
    38641 actttataat gaagtcttat aatagaaaag tctgtggtga aaatttaaag aaaatagttt
    38701 aattatgtta taagaattta ttgagttaaa attagacatt ggcagaaaat taaatacaaa
    38761 taagtatcta taaggctata ttaaaatatt attaattttt tattttaata tagccttata
    38821 ttgttatcaa taaatgaaaa atattgtaac aacagaagaa caatagtatt atgtttatag
    38881 ataaaaaact tattaggagg taaatttatg ccaacaataa cattaaaaaa tggtgtagac
    38941 gtacttatta gagaaggtgt aagagaagat gcttaaagca ttatagattt ttataatgaa
    39001 gtgggtggag aaactcattt tctgtcattt ggaaaggatg aatataaaat atctttggag
    39061 gaacaagaaa atgctataga atcagcaaaa gcatctgata attctgttaa actgattgca
    39121 tttatagatg atgaaatagt tgggattgca acgattgatt ctaatcaaaa agcaaaagga
    39181 aagcatgttg gagtattagg aattgttgta aaagaaaaat attggggaat tggattgggt
    39241 aaaaggctta tgttagacct tatagaatgg tgtaaatcaa atggaataac caagaagata
    39301 actcttgtca ctaatgaaga aaattataat gctataggat tatacaaaaa agttggattt
    39361 gaagtagaaa gcatactaaa aaaagaatgt tattataatg gtgtttatac tgatttaata
    39421 ggaatgtcac ttttattggg aatataatta gtgtacattt tatctcgaaa aaagtatatt
    39481 aagtatggta taattaaaaa agaatataac ttaatataat tttggaggac gaattatgaa
    39541 aaatctgtgt aaaatatcag atgattattt aaacagcaag cttaagtatt taaagttatt
    39601 atcaaaacaa tatccaagta tatcaaaagc aagtacagaa ataataaatt tagaggctat
    39661 attaaatctt ccaaagggaa cagagcattt tataacagat gtacatggtg aatatgaacc
    39721 atttgtacat gtattaaaaa atggttctgg tgtaataaag agaaaaatag aagaattgtt
    39781 ttcaaataca ataagagaca gcgaaaaaaa aatgttagca actcttgtat attatcctga
    39841 acaaaagtta gacctaataa ttaagcaaga ggaaaatata gatgattttt atagaataaa
    39901 tatttataga cttatagaac tttgtaaata tgcttctagc aaatatacta ggtctaaagt
    39961 tagaaaactg ttaccagaaa attttaaata cataatagaa gaacttttgc atgagcatgt
    40021 taaaagtgaa cataaagaag agtattataa aagtatagtt gaaacaatag ttgacatagg
    40081 aatagctaag gagtttataa ttgctatttc tacagttata caaaaattgg ttgtagatag
    40141 acttcatgta ataggagata tttatgatag aggtccaaga ccagatataa ttgtcgataa
    40201 gcttattgaa catcattgtg tggatattca atggggaaat catgatatat tgtggatggg
    40261 tgcagcctca ggtgaaaaaa cttgtatagc aaatgcactg aggatatcag ctagatatgc
    40321 gaatttagac attgtagaag acatatatgg aataaatctg ttgcctttag ccacttttgc
    40381 tatagaaatg tataaagatg acccttgtaa agaatttatt ccaaaggtta atgaccaaag
    40441 tgtaacaaca acagaaaagt ctttaatggc aaaaatgcac aaagcaataa gtataattca
    40501 atttaagctt gaaggtgagg ttataagacg aagacctgaa tttgaaatgg aacatagact
    40561 tttgcttaat atgataaact atgatgaagg tacgattact ttaaaaggta aaacatataa
    40621 attaaaagat acttatttac ctacaataga caaaaaagac ccatataaat taactatgga
    40681 agaaagaaat gttattgata aattagtatc ttcatttaga ggtagtgaaa aattacaaaa
    40741 acatgtttca tttttgtttt ctaaaggaag tatatattta aaggctaact caaatttact
    40801 gattcatgga tgtgtaccat taaatgaaga tggaagcttt atgtctatga atataatggg
    40861 aaaagagtat aaaggtaagg ctcttatgga taagatggag tctctagcaa gagaaggatt
    40921 tttctttaaa gataaggcag aagaaaaact atatggtatg gatattatgt ggtatttatg
    40981 gactgggaaa tgttcatcat tatttggtaa agatgatatg actacatttg aaagatactt
    41041 tatagaagaa aaagagactc ataaagaaaa taagaatcca tattttaaac ttagagaaaa
    41101 tgagatggct tgtaaaagat tatttgaaga atttgattta gagcttgatg aatcccatat
    41161 aataaatggt catgttcctg tggagagtaa aaatggagag agcccaataa aagctaatgg
    41221 taaaattctc gttatagatg gtggtttttc tagggcttac caaaaaacta ctggtatagc
    41281 tggatacact ttgatataca attctagaac tttgcaatta gtatcgcatg aaccatttaa
    41341 ttctgctgaa gaagccattg caaatgaaag tgatatctta tcgacaactg ttgtagtaga
    41401 gcataaggca aagagaaaaa tggtgagaga tactgatgaa ggagtaaaaa tacaagagga
    41461 aatagaagat ttaaaactac ttttaatggc ttataagaaa gggctaataa aagaaatgta
    41521 gatattatag aggcacttca taattgaagt gtcttcttgt taatacttat taaatatatt
    41581 ataaagatta ataatagtag tttatattat acttactaag tgaatccaag ataaatgggt
    41641 tcactttttt tatttataaa cattattaaa aaaatataga tgttttggtt atattccttg
    41701 tcctttataa atagaattat ataaacaaag gaggaggaca agagatgaat aaactttctg
    41761 atgaaataat aaactctctt tctacaacta taagagaaaa ggtagaaaaa gtctcaaaca
    41821 ataatctgaa catagaagaa attaggttac gctcacaaaa accattgata ctaaatgcaa
    41881 actctaaaga ttatttttac aatcaaaaaa ctatgacact tgatttaaat caacaaaact
    41941 catatgtagt aacaagagaa gatgtagagc aaacttttca aataatatgt aaatattcaa
    42001 tacattcatt tatggatgac ataaaaaaag gattcataac tttgagagga ggtcatagag
    42061 tagggttagt tggaaaagca atagtagaag atggacaagt caaaaatata aagcatatat
    42121 catctttaaa tataagggta tcaagggaaa ttattggatg ctcggataag attttaagtc
    42181 atattataaa gggaaaaaat cagattaata atactttgat aatatcacca cctcaatgtg
    42241 gaaaaacaac tttaataaga gatatagtaa gaaatttaag taatggtaat gaagattatg
    42301 gatttaaagg tttaaaagta gctttggtag atgagcgtaa tgagatagca ggagcttatc
    42361 taggtgttcc acaaatggat gtgggaataa gaactgatat aatagaaact tgcccaaaag
    42421 atttgggtat tacgatgctt ttaaggtcta tgtcaccaaa tgttattgtg acagatgaaa
    42481 ttgggagtga aaaagaaata aaggcattat acacagcatt aaatggaggt ataggattaa
    42541 taactactgt gcatggagat tcaatagatg atatacaaaa tagaaaagaa ttaaatagac
    42601 ttctggataa agagttattt aaaaaggtta taattctttc tgcaaaaaga ggagcaggaa
    42661 cgattgaaaa aatttatgat ttagaagaaa agagatggta ttttgcaaat taaaataatt
    42721 attatagctc tcttggtagg aagtagttat ttgataggtg aacaaatata caaaacttat
    42781 actcgtagac ataaacaatt aaacgattta ataagagttt tagaaatttt gaggatggat
    42841 ttatctttcg gactttatac attagaagaa atctttaata ggataggtgg aaataaagag
    42901 ttttgttttt ggaaattttt ctatcaaata tcagaaggac ttcacaatga acaaagtaaa
    42961 actttggaaa taataatatc agaaaatatt gatgtcttaa gtaaagaaac ttatctaggc
    43021 aataaagaaa ttgaggaact gaaaaatcta atacttactt tagggaaaag cgatatagag
    43081 tcacaacaaa gaatgataga tttatctata gaaaatttga aaaaacaaac ctatgagaca
    43141 aaggaagata taaacaaaaa aggcgtattg tataaaaaat tagtgacatt cataggtata
    43201 ggtatatgta ttatattgat ttaatatggg gaggtttatt atggaaatat cattgatatt
    43261 aaaggttgca ggtgtcggta ttctaatatc agtattgaat atgatattgg aaaaaacaga
    43321 tagaaaagat tgggcaggtc ttactacatt agcaggtgta ataatcgtat tgggtatggt
    43381 aataacagaa ataagtgact tgtttaatac tgtaagaaca atgtttcaac tttactaaaa
    43441 aggagggagt aatttggaaa taatgcaatt aataggaatt gcaatcattt caactacact
    43501 ctgtttagta attaaaaaag atagaccaga gatagcaaat ttcatagcaa taataactgg
    43561 agtaataata ctattgtcag ttatgtttaa gctaaatttt atagtagata gtatacaaga
    43621 tttggctaac aaggctaaca taccgactat gtatatatca ttaataataa aattaattgg
    43681 gatagcatac ttaatggaat ttgcgataca actttgtaaa gattgtgggg aaggaaacat
    43741 tgcttcaaaa ctagaatttg gaggtaaaat aatagttatg tcaatgtctt tccctatact
    43801 tttgtccatt gtagaaatgg tggtgaatat aattccatag atgtgtatct tggaatgaat
    43861 ataagtatat tagagaactt tcgttgtatt gaggtttata gagtgaagag aggcatggtt
    43921 gcttatgaaa aaaagatttt taccaatgat tataggtttt ttatttactt tcttttttgt
    43981 aaatatcttt gctattatga tatttgctaa tgaagtacca agtagtgaag ataaagaata
    44041 tggtgaaaca aaaaatagca tagataaata tatagatggt caattagaca agctagatat
    44101 aaatgagatt caagactata taaataaaga aatagttatt aatgatgtta atttaaaatc
    44161 atttgtaaaa gatttaatta gtggagaaaa aaatatctta gatttattca ataaagatgg
    44221 attgaagata ttgatgtttg atgaatttaa agcaagttta aaggtagtag cagtaatttt
    44281 ggtactggct ctgttatcat ctattttaaa gagtctggaa aactcttttt catcaggtgc
    44341 agtaagtcaa atagcaacat atataatatt tatcacaatg gtatcactta cacttattgg
    44401 ttttaaagat gtattacaga tttgttatga tgcaatagac catactgtag gtcttatgca
    44461 agttataatg ccaatattaa taacattttt acttttaatt gggttcccaa taacgtcgac
    44521 tactttgaat cctatcttca taggaggagt aacatttata aatgtatttt ttaaaaattt
    44581 tctatttgta tctattactg ttgcatttgg aattttaata ataaacaatt tatcaaaaaa
    44641 tataaggcta aagagatttt tctcctttgt aaaacaaata aattatgttt ctattggagc
    44701 tatgtttact gtttacttag gtcttgtatc catacaagga ttgtatgtaa ccagttttga
    44761 taaattcagt gtaaagactg ctaaatttgc tataggtaat tttattcctg ttgtaggtgg
    44821 atttgtatct gactctgtgg atattttatt atcttcatct cagcttataa aaaatatatt
    44881 tggaggaata gggcttatct tacttgttgg aatttgttta cttccagtaa taaaaatttt
    44941 atcagtaata gtagtgtaca aattagcagc aataattgta gagcctgttg gagaggatgg
    45001 aatatctaat tttttaaatg aagtggcaaa tttaatgata ataatgctag catcagtaat
    45061 tgctataaca gtaatgttct ttgtaactgt agctatcttg acctcgataa gtgtcgtaag
    45121 tcaagggtaa tttatatatc agtgaaaaat aattacattt aatctaatgt ttttagagag
    45181 gaggatgcta tgttagaggg cataaaggca tggatagtta gtgttttgat aggagcattt
    45241 atagtaaata tagtagatat gattttacca agttctaaga taaaacctta tgtcaaccta
    45301 gttttaaact ttatgtttgt atttattgta ataactccag tagtaggatt tttttcaaaa
    45361 gatatgagtt tagaagatag aatattaaaa tctatgggaa actacaacaa gcagtatgta
    45421 gatagtacaa atgctttggc aaaagagact ggaaataata gtctatccaa gggatatgag
    45481 gatgggttaa aagaagtact taaattaaaa cttgatgaat atggttatga tttagaagat
    45541 atagagctta atggggcaaa tataaataat ataaaaataa aagaaaagaa taatagtgca
    45601 aaaagtaata acagttccaa tgaagaaaat aaaaataata gtactaaaag agaggaagaa
    45661 aacataaact ctaatgacaa agaaaattct aaacaagtct tcaaaaaagg tacagagtat
    45721 gggttgaacc taaatgaaga aaaattaaaa aacgacttaa taaaggtgct ggatgtttct
    45781 atagaagata tacaaattga taaataggag ttgggagaat gttgaaaaac ttaaatgaca
    45841 aagataagag aaaggtatat tcactaataa ctatagcagg aatatgtgtg gtttcattag
    45901 tattattatc ttgttttcct agtagcaaaa gtgataaaca agttggaaaa acagagacta
    45961 ataaaaatgc tgaaaagcaa gtcactaaaa aacaagaaaa agatgattta gagtcaaaat
    46021 taacagcaat actatctaaa attgatggtg caggagacgt tgatgtaatg gttacatttg
    46081 aatcaagtga agaaatacaa cctgctttta attcaaataa tacaacagaa acaacagagg
    46141 aaaaagatgc acaaggtgga gaaagaacag ttacgacttc tagtgaaaat aaaacaatga
    46201 taacttcaaa ttcaagtgac cctgtagtta taaaaactac agagccaaag ataaagggag
    46261 tgatagtggt agcaagtgga gcaagtgacc caagtgttaa agagacactt tatagtgcag
    46321 tacaaacatc attacaagta gctggtcatc aagtagaaat atattctaaa taaatacata
    46381 taaccggggg gataaatgaa tgaagtttaa ttataaggga agaggatttg taataataac
    46441 tttaactgca atgttagtgg tagtaggaac agtgaattat caactaagta agaaatcttt
    46501 attagaaaca tcaaaagagt ttaaggcata tgaagaagca cagttgcaaa aaaatactga
    46561 tgatagtgat agtagtaaga cagaggattc tagtaatgta gataaacaag gtggtaagga
    46621 aagtgctgat attgatatag tcgatagcaa agctagtaaa gtaaaagaaa aggcaactga
    46681 gacaagtaag gaaataaaag ctcaattatc atcagagaaa aatatgaaaa aggcatctta
    46741 tattttagat atgaaaatga atagagaaaa acaaagaaat gagttagttc aagatttaaa
    46801 tgaaatgata aataatccat ctacaacaga agaatctaga aaagaagctt ctaatatgaa
    46861 attaaatata gttaaaattc aagaaaaaga acttcaaata gaaaaccttt tgagtacaaa
    46921 aggatatgaa gaagcgttag tttatataag cgataacaaa gttaatgtag ttgtaaatga
    46981 agctaaatta gaaaagaaag atgcagcaaa aatatttgat ttagttgcag aacaagccaa
    47041 tgtgaagtat gaaaatatta aacttacaaa taataatagt aataagtaaa gtataaacct
    47101 cagaattaac tgaggttatt ttcttgcaaa gtagacttgt atttggtatt attatactta
    47161 taaaaatagt tccttaaaat caggaggtaa gttttaatat ggaagataat aaatttggac
    47221 aagtaaaaat atctaatgat gtaatagcta caatagctgg tttagcagca ttagaagttg
    47281 aaggcataga aactacagca acattaacag ataagttgtt aaaaaataat ggagtaaaaa
    47341 tacaaatcga agaagaggat gttaatttag atgtaatggt tacgataaag tatggaatgt
    47401 caataccaga tacagctttt aaagttcaag aaaatgtaaa aaatactgtt gagacaatga
    47461 caggactgaa ggtttctcaa gtaaatatac acattcaagg aatcagcttt aagaaggata
    47521 aagttgataa agaagaagca aaagcaacta agaaaaatta atattagccc tctgaaaagt
    47581 cagagggttt tttgctggta attgatttta tatggataat taaacttaaa ggagaatttt
    47641 gattgatgaa aaaagatagg gcacaaaaga gtacaacaag agaatatata atgaaactta
    47701 tataccaaat aaatattaat aaagaagatt ttgaaacttt agaagataaa gtagataatt
    47761 ttttaaaaga taattcagaa catattataa atagatataa agaacttgca cttcaatact
    47821 caaaaaatac aaatttaaag ttagaagata ctgaaattga agatgttata gacaagaaat
    47881 atataaatac agtatgcaaa gcattaaaag aaaatcatga caaaatagat gagttaataa
    47941 ataagcatgc taaaaactgg acagtagata gaatgccaaa ggtagatgtt tctatactta
    48001 gattgtctgt gtgtgaaata ctttatttag acactcctaa taaagtatca ataaatgaag
    48061 cggtagaact tgcaaaaata tattgtgatg ataagtctcc taaatttata aatggtatat
    48121 tgggaagtgt tgttgatgaa attgggaaat aatataataa ttggaattga tactagctgc
    48181 tatactactt ctatagcagc tatctcttta gataagaaag ttatattcaa tgaaaaaata
    48241 atgttagaag taagggataa ttcaaaaggt ttaaggcaaa gtgaagctgt atttcaacat
    48301 atcaataatt tagggattct tagtgatagg ataaaatctt ttaaagataa gtttaatgta
    48361 gaaggagttt gctcatcaaa aaaaccgaga cctgttgaaa actcatatat gcctgtattc
    48421 aatgtaggtc ataattttgg aaaattattg tctagtatat atggttgtag gttttatgag
    48481 actactcatc aagagaatca tattgaagca agtttattaa atagtaaatt aaaaaataat
    48541 aataaattta tttctgttca tatgtctggt ggtactacag agatactttt aacaagcaaa
    48601 caagatagtc atcacaatgt atgtgatact aatttaggta aaattgccaa aataagtatt
    48661 aaaaaagatg ataaaagtaa gttatacaat aattttggat ataatataga tataattgga
    48721 ggaagtaaag atattagctt tggtcaactc atagatagag taggaattaa gcttggatat
    48781 aaatttcctt caggaaagta tttggatgaa aatgctttaa attgtaatct taaaatagaa
    48841 agtggtctga aaacttctgt aagagatggg tatatgaatt tatctggatt agaaaatcag
    48901 gtaaataaga ttataaatga taatggagat aatactaatc aaaaagaata catatctaaa
    48961 ttagtattag attcagtagt tagaaatatg tttaaatctt tagtatattt atgtgaaact
    49021 tataatgtaa atgaagtagt atttgctggt ggagtttctg caagtaagta tattcttaga
    49081 gaattaagca tgaagttaag aaaaaagcat atagaagctt attttacaga accacaatat
    49141 tcaactgata atgctgtcgg gtgtgctata ataggattga ataacttttt aggagaaaga
    49201 gtatgaaact aagagcttta gatataagtg aagcaaactc atatattaaa agaattttaa
    49261 taaacgaccc aatactttct aatcttaaag ttaaaggaga aatttctaat tttaaggttc
    49321 atagtagtgg gaatgtatat ttatcactta aagatgaaac atcaaagtta aactgtgtaa
    49381 tttttaaaag taattttaat cgcaatttaa aattagataa tggtgtaaaa attatagcta
    49441 atggatatat atcagtctat gaaagagatg gtgcgtatca attatacatt aatgaaattg
    49501 aaatagaggg aataggaaat ttacacatag agtttaatag attaaaggaa aaacttaata
    49561 aagaaggatt atttgaccct aaatacaaga tacctatacc taaaatgcca aactcaattg
    49621 gcgttataac atctccaaca ggtgctgtta taagggatat aataaatgta ataaaaagaa
    49681 gatatccgaa agttaatata aagctttatc cagtaatggt tcaaggagat aaatcagctg
    49741 aagaaatctg tgaagcaatt agatttttca atcatatgaa gaatgttgat acacttatag
    49801 ttggtagagg tgggggttca atagaagaac tttggtcttt taatgatgaa atggtagcaa
    49861 gagaggtttt taattctcaa attccgataa tatcagcagt tgggcatgaa acggatttta
    49921 ctatttgtga ctttgtgtct gatatgagag ccccaacgcc atcagcagca gctgagattg
    49981 ctactccatc attggatgat ataaactata aacttggaaa cataaaaagt aggatgagta
    50041 agtctcttac aaatcaaata gagctagacc aatataggtt agaaacagta tttaataaga
    50101 taaataacta tttagattcg tacacaataa aagataaagt tatacaatta gacaaaatat
    50161 atgataaaat aatttttgga atagaaaata atttaaaatt agaagatgaa aaattagtaa
    50221 aaattggagc attactccat aatttaagtc cattagctac tatggataga ggttatagta
    50281 taactcaaaa aaatggaaaa gtaataaata gtataaaagg attaaaaata aaggatagta
    50341 tagatatagt attaaaagat ggaaatttag aatgtatgat agataaaatt gaaaataaag
    50401 agggatagta tgaatttaac ctacgaagaa gcttataaaa gattagaaag tattttaagt
    50461 gaattagaat ctaaaaatgc tagcttggat gaatctttaa gcctatatga agaaggtata
    50521 agcctttata aacattgtaa taagctttta gatgatgcta aattgaaaat aagtaaattt
    50581 aatcaattag gaatagaaga agactttaaa atagaggagg aataaaattg gaatttaaac
    50641 aatgtctaaa agaaaaagca agttttgtag aaaaagtact taaagaatat atgcctaaag
    50701 aagaagggta tcaaaaaact gttattgaag ctatgaacta tagtttaagt gctggaggta
    50761 aaagattaag accaatactt acattggagg cttgtaaaat agttggagga aatgaagatg
    50821 aagctattcc ttttgctatt gcaattgaaa tgatacatac atattcacta atacatgatg
    50881 atttgccagc tcttgacaat gatgatttaa gaagaggaag accaacaaat cataaagtat
    50941 atggagaagc tatggggatt ttagcaggag atgctttgtt aaactatgct tttgaagtaa
    51001 tgcttgcagg ctcaatcaat aaagaaaatc cagaaaagta tctaaaagct ataaatgaaa
    51061 ttgccaaagg tgctggtata tatggaatga taggtggtca agtagtagat gtagaaagcg
    51121 aaaataaaca aattgaaaaa gaaaaattag attatataca tatgaataaa actgctgcaa
    51181 tgatggtagg ctgtatgaga gcaggagcta ctataggtgg agcaaattct gaacaaatgg
    51241 aagaaataac taaatatgca aaaaatatcg gattatcatt ccagatagta gatgatatac
    51301 tagacatagt aggagatgaa gctaagttag gtaaaaaggt tggaagtgat atagaaaatc
    51361 ataagtcaac atacccatct cttttaggtt tagataaatc taaagaaatt gcacataatt
    51421 tgattgatga agcaaagaaa agtatagaaa aattatctga tgatgtagat tttttaaagg
    51481 gattggctga atatataata gatagagaat actaaaatat acagctaaaa tcgaggaggg
    51541 aaaaatggat tttttttcgg agatttttaa caatggggct ttgggaataa gtctgattgc
    51601 ttgtttctta gcacaattta taaaaatatt tactggtaag gaaaaaagaa ttgagttatc
    51661 aagaatactt atttcaggag gtatgcctag ttcccatagt tcttttgtta caagtttagc
    51721 tacagtggta ggtattgaaa aagggtttaa ttctactgat tttgctataa ttaccgtact
    51781 tgccttaatt ataatgtatg atgcagcagg tgttagacga gctgttggta aacaagctac
    51841 tatattaaat caaatggtgg cagatataca gcatggaaag catatagagc agaagaaatt
    51901 aaaagaatta ataggtcata caccactaga agtttggttt ggagctttac ttggaatagt
    51961 aacagcttta atattgatgt aaattgaaaa tttacagaat aaaggtgagg ataatatgta
    52021 taaatattta gataaagtaa attctccaaa ggatataaaa aatatgagta tagaagaaat
    52081 ggatttactt gcaaaggata taagaaagtt cttagtaaaa tctgtttcaa agacaggagg
    52141 acacttagct tctaatctag gagtggttga gttaacatta gcacttcata aagtatttga
    52201 tagccctaaa gataagattg tatgggatgt agggcatcaa tcttatgttc ataaaatagt
    52261 tactggaaga aaagattgtt ttgtatcttt gagacagttt aatggactaa gtggttttcc
    52321 aaaggagaat gaaagtcctc atgatatttt tgatacagga catagcagta catctatttc
    52381 tatagctaca ggtattgcct gcgctaggga tataaaaaaa gaaaactata gtgttatatc
    52441 tgtgatagga gatggttcta taacaggagg tatggcactt gaagctttaa atcaattagg
    52501 atatatagat accaatatga tagtaatcct taatgataat gagatgtcca tagataaaaa
    52561 tgttggagga atgtctaaat atctgtcaag tattataaga aactcaacag ttgagaaaat
    52621 gacagatgaa gttgataaaa ttttaaatgt tacacaaaca ggtgaaattt tatctaagac
    52681 tgcacataga tttaaagata aactaatgta tagcttttct cctcaggact gttcattttt
    52741 tgattcatta ggtataagat attatggacc tatagatggc cataatacta aagaactaat
    52801 agatatattg agaaaagcta aacacaaaaa agggcctgtt cttttacatg taattacgaa
    52861 aaaaggaaaa gggtacagat ttgcagaaga acaacctgat aaatatcatg gagtatcaaa
    52921 gtttgatata aagacaggtg ttacatctgc aaaagttaag tctatgtcaa ttagtgttgg
    52981 agaaaaactt gttgatatgg ctaataataa tgaagatata gtagcaataa cagctgcaat
    53041 gccatctggt acagggttaa acctgtttga aagtgcatat ccaaaaagat attatgatgt
    53101 tggtatagct gaacaacatg ccacaggatt tgcagcaggt cttgcaaaaa atggtatgaa
    53161 accttacttt gctgtgtact catcattttt gcaaagggca tatgaccaag ttattcatga
    53221 tgtatgcata acaaaaaagc cagttacttt tcttatagat agagctggat tagttggaaa
    53281 tgatggagaa actcatcatg gtatgtttga tttaagttac ttaaattcta ttccaaatat
    53341 tgtagtaatg gctccaaaag acacaagaga gatggagctt atgatggatt tgtcattaaa
    53401 attagattgt ccactagcaa ttagataccc aagaggaagt agttattacc tagataaggg
    53461 agaatatgga gagattgtac tgggtaaata tgaggtatta gatgacggtc aagatacagt
    53521 catactttgt attggaagta tggtaaaaca tgctttagag gctaaagaaa tattatcaag
    53581 agaaggaata aatccgacaa tagttaatgc tagattctta aagccaattg atgaaggtat
    53641 gttaaaagca ttattaaaaa atcataaaaa tgtagttact atagaggata atattgtaac
    53701 tggaggattt ggaagtagaa taaacaagtt tattatagat aatgaatata acgtaaatat
    53761 acttaatata gcaataccag aagaatttgt aaaacatggc aatatagatg aattatatga
    53821 ttttgtagga ttatcgccaa aaagtattgc tgataaaata aggaaattag ttattgagta
    53881 aaaatctaat gtagatattg taaattaaaa tttatataaa atatagaatt tatcaattag
    53941 aataaatatc aagtgtgtta cttcaagcta ctttagttat cattaaagta gcttttaaca
    54001 gaaaggtaat tgtatgaaaa aaagaataga tttattatta gtagaacaag ggtactttga
    54061 aagtagagaa agagccaaaa aagcaataat ggctggattg gtttttgttg ataatcaaag
    54121 gtgtgataag gctggtacag aagtaaaaga agattgtagt atagaagtaa aaggaaatcc
    54181 aataccatat gtcagtagag gtggtttaaa gcttgaaaaa gcaatgaaaa actttgattt
    54241 gactattgat ggaaaggtat gtatggatat aggggcatcc acaggtggtt ttacagattg
    54301 tatgctcaaa aatggagcaa taaaagtatt ttctatagat gttggttatg gtcaacttgc
    54361 atggaaattg agacaagatg atagggttgt gtgtatggaa agaactaata taagaaatgt
    54421 tacaatagaa tatacaaagc agtttgctga ttttgcgtca attgatgtgt cttttatatc
    54481 gttaaagtta gtacttccaa aggcaaagga gttagtacgt catgatgggg aagttgtcgc
    54541 tttaattaaa cctcaatttg aagcaggaag agaaaaagta ggtaaaaaag gagtagttag
    54601 agaaaaatct acacatatag aagttataaa aatgatttct gatttctcag tcgaaaatgg
    54661 atttgagatt ttagggttag atttttctcc tattaaaggt cctgaaggta atatagaata
    54721 tctaattcat ttgagaaatg gaaatgaagg atatgaattt gatggtgaga catacaataa
    54781 caaaatagta gaagttgttg aagcttctca taatttagac aaataggtta aaagggggct
    54841 tagatttgat cctcgaatta tatatgaaaa attgtgcctt agttgaagaa ctaaggttga
    54901 atattgataa aaacttaaat atacttacag gtgaaactgg ttcaggaaaa tctataataa
    54961 tagatgcact tggactttgt ttaggtgata aatatgatag gtctttttta agaaaaggaa
    55021 cagataaggg acttgttgag gctgtatttt tttcagacaa tagatatctt aaaaaaatat
    55081 tagaagaaaa tgatataagt atggaagatg ataatttatt ggtaataact aggttgatat
    55141 actcagatgg taagagtact gctagagtaa atggaaggac tgttaaagtg tctcttctta
    55201 aagaaattgc atctacactt atagatatac atggtcaaca tcaaaatcaa gctttgttca
    55261 ataaagatac tcatttaaaa tttctagacc tttttggaga aaatgaattg gaagagttta
    55321 aaattgctta taaaaatgtg taccataagt atagtgaagt aaaaaaagct ttaaattgct
    55381 tgacagaaaa taaagatgag atgcaaattc aacgagaaat agatttatta agatttcaaa
    55441 taaatgaaat tgaagctgca aatctaaata agaatgaata tgaagattta ttaaaacaaa
    55501 gagaagttta tagaaatagt gaaaagatat ataataatct aaattcgagt tatagcaagt
    55561 tacataatgg tgagtataat gttattgacc taataggtct tgcatctaag gaacttaatg
    55621 acatatctaa atatgacagt gttttaagtg aatatagtga tactgtagaa agaattatgt
    55681 atgagttaca agacatatct ggtgagataa gaaattacaa agataatata gactttgaac
    55741 cttatgagct agagcagatt gagcttagaa tcgacgaaat taataattta agaagaaaat
    55801 atggagacag tatagatgat atatttgagt attatggaaa aattaaagat agattagatg
    55861 aaatattaaa tagagatgaa agagttgaac agttaaaaag caaactgatg aatatagaag
    55921 aagatttaaa aataaaagcc agtaaattga ctaaagcaag aatagaagta gcaactactt
    55981 tagaaaaaat acttcttgat gaattaaaaa gcttaaatat gaaaaatgta atgttcaagg
    56041 ttaattttga aaaaggttca tttactttag atggagtaga tgatattgaa tttatgatat
    56101 cttttaactt aggagaagat attaagccta tatataaagt tgcatcaggt ggtgagatgt
    56161 ccagatttat gttagcgttt aaaaccatat tggctgatat agatgatatt gatacattag
    56221 tgtttgatga gatagatact ggaataagtg ggatagcagc acagatagtt ggtgagaaat
    56281 tgagtgatat agcaaagaaa aaacagataa tatgtataac tcatttacct caaattgctg
    56341 caaatgcaga cactcattat tgtatagaga aagatacttc aaataatagg acgtttacca
    56401 atgttagtaa gttaaatgaa tctcaacgaa aaaatgaaat tgccagactt atagcaggta
    56461 acaatataac agaaaaaact attgaacatg caagtgaaat cattgaattg gctaaaaaat
    56521 gttagtgaaa aacaagcatt ttaataggtg taaagaattt ttatagaaat gctttacacc
    56581 tattttttat gatgaaatta ttttaaaata tctagtgcct atattgaaga aaagtaggtt
    56641 tgaaaatgga atgaactttg tataaatttg taactttgaa atttctaaat gcttaagtat
    56701 aatattattt attaaggtag tagtgagggg aggcataaaa tgaataaaaa agatactatg
    56761 cacttaattt ttataattgt aatttttatt ttattatggt atattgtggt agcagtaagt
    56821 gagattaata aaaatattga aagttgtagt aacaaattag attatttgtc acaagagctt
    56881 atgagtacta agtcggatat tagtaatact ataaatgaag aaatgtcaaa aagttatata
    56941 acaaaaagta ttgacttaaa agtaaaaaaa atcgagaaaa aagaatgtac tattgatgta
    57001 gatgttcaat taagtaaact tggaaaaaat ggaaaagtat tttttatgta taaagaagat
    57061 agtgataaat ggaatgaaac tgagatgaca aaacatgggg aattatcata tgcatgtgaa
    57121 ataaagataa atacaggaag tgaatatgat tataaagtag ttacaagtgg agatatatct
    57181 gaaagttctg atgtacaaaa actgactaaa tctgattata tgcctgaaca acctatattt
    57241 aattctggga taagagataa tagagaatac tttattgaaa tagtagaaaa tagtaatctt
    57301 tttaatcatg agagtgaaaa gtcaaaaaat aaagcttacg ataatatcag gttagaaaaa
    57361 gtagatatta ttgtaaatga aggaaaaaaa gacactatat acaaagccca attagctgaa
    57421 ggcttagatg atggcaaaac taataattca aatagaaatc aagtgaatta tgaagtaagt
    57481 tttcctaaaa gggatattga tgacataatt tatattaaag ctaaacttac atacaataat
    57541 ggagtagaat atacaaagga tataactgaa gatataacta tgggattaca agatttagaa
    57601 aaataaatac atatttaaat aaatattcaa atagatttgt tcacaaatag agtataaata
    57661 aaactgtagt agttttactt atactctatt aaaaaattat aattttaatt ccatatatct
    57721 agccccttca attggattat aagtataaag ttctatatca taaaaaccaa acttttcata
    57781 taatccttgt gcacttttca tgcttggaag agtatctaat ctcatgtgtg tatatccaat
    57841 ttttttagct tctacaatga tttcttccaa tagtattttt cctattttta atcctctaaa
    57901 ttgatttctt acatataatc tttttaattc acatatttta ccttcaagtt tttttaaagc
    57961 aacacaacct gccaaatttt catcaacaaa tgccaatatc aatgagcctg aaggtttttt
    58021 atattttcct ggaagtgttt ttaattcatt attaaagtct tgaaaacata agtctatatt
    58081 tagagaattt gaatattcag taaatagaag ttttacgttt tcaagatttt ctaaatcttt
    58141 ttcttcaaca aattgtaaag ttatattttg agccatttta tccgccatta tataaacatg
    58201 tttatataat gacttcacct ccttgatata tgtatatttt tcttaatatc aattagattt
    58261 taataccatt actttttcac caccatagtc aagctctcca ttaaacttaa atccaaaatc
    58321 tttataaagc ttaatagcca ttttattgtc ttcgaatata ctaagatata gttcatcaca
    58381 gttatattca tttttaagat gatttacaag aaaatctaat gatgcttttc cacatccttt
    58441 accttgatat tttgaatcaa tcataaatct atcaagccaa actctatcat cttctaaacg
    58501 accatacata gcaaatccta ttaaagaatt ttcatcatat attccaacag gtacccaagc
    58561 tgttataaat ttagattcag caatagaaag tgcattgctt tctatgtaat ctaactgact
    58621 tttatctaca gacaaactaa ctaaatcgtg ccaattattt atgtctgcaa ttcttacatt
    58681 taatttcaag atattaccgt ccttcaaact ttttgttaca atctaatact atcatattat
    58741 gtattaaaaa tcacttatta agattttaaa taaacttttt gaatgaataa attctcagga
    58801 aaaaggaatt ataaatacat aataatacag cataggagta gatggatata tgaacaaaat
    58861 aaataaaaat ttcaaaaata tttggataat attaggagct gtatcgattt ttaccatggt
    58921 tataattttt aataaaaatt gtaatgttaa taaagagact caaatagaag cttttacaat
    58981 taagaatcat gataaaaaat ttgtatatcc tatgggaaat ataattggag taaaagcaaa
    59041 tacagatggt gtattggtct taggatatga ggaagaggat gtaggctata ttggaggcat
    59101 acaaataggt gataatatag taaaaataga taataaaagg ataaaaaata gtcaagatgt
    59161 atcagagatt ttgaataaga ttaaaaaaag taaagttgaa gtgacttttg agagaaaaaa
    59221 taagtataaa acagaagtaa tagagactaa aaaagaaaat ggcaagtata aacttggatt
    59281 atgggtaaga gataaaatat ctggaattgg aactatgact ttttacgacc ctagtatgga
    59341 aaagttcaaa gcaatcggtc atgccataaa agattcggat actaatgaac tattaaagat
    59401 aaagcaaggc tatatatata agccagaaca attaaaaata gtaaaagcta gtaatgaaaa
    59461 agtaggtaaa ataaaaggag attttaatga cagcaattta atgggtaatt tctcaaataa
    59521 ttcagagcta ggtataagtg gtaatattac agaaaaccat aataaagagt ttaatgtagc
    59581 aaataaagaa aagcaattaa tagaagttgg aagaccgcaa gatgtaaaaa taggagatgc
    59641 agtaattctt tttgaggata aaaacaaaaa tataacaagc tatgatataa aaatagaaag
    59701 tatagtatat gataaaggaa attatagaga tatggtaata aaagtagtag atgacaagtt
    59761 attggaatac acaggaggta tcgtacaggg gatgagtgga gctccaataa tacaaaataa
    59821 taaaattatt ggtgcaataa ctcatgtttt tagagataat ccgaaaaaag gatatggtat
    59881 ttttatagat gaaatgataa aattgtaggt gaggcattaa aaaattttat tattttatca
    59941 attatctagg aggaatataa ttttggagtg tcgaatatgc tttagagtag ataattagga
    60001 agcaattgtg taaaaagttt agttttctgt aataagaaga tgttttttaa tggggggatt
    60061 tttagtggaa aaaatcaaaa tagttttagc agatgacaat aaggattttt gtcaggtatt
    60121 aaaagagtat ttgtctaatg aagatgatat cgatatatta ggcatagcta aggatggaat
    60181 tgaagcatta gacttagtaa aaaagacaca accggactta ttaatactag atgtaataat
    60241 gccacatcta gatggattag gtgtaattga aaaattgaat actatggata tacctaaaat
    60301 gccaaaaata atagtactat cagcagtagg tcaagataag ataactcaaa gcgcaataaa
    60361 tctaggagca gattactaca tagtaaagcc atttgatttt gttgtgttta taaacagaat
    60421 tagagagcta gtttctaata gagttacaca agttgagcca aaacctagac cagttcaaga
    60481 gactcaaatg actagaagtg attttgttaa gaatgtagga aatatagaga cagaaatcac
    60541 aaatataata catgaaatag gagtaccagc tcatataaaa ggatatttat atttaagaga
    60601 agcaataaaa atggttattg ataatgttga gcttttaggt gcagtaacaa aagagttata
    60661 tccaagtata gctaaaaaat tcaatacaac accaagtaga gttgaaagag caataagaca
    60721 tgcaatagag gttgcatgga gtagaggaaa agttgacaca ataaatcaat tatttggata
    60781 tacggtacac aatactaaag gaaaaccaac taattcagaa tttatagcaa tgattgctga
    60841 taaattaaga ctagaacata gtatggttaa ataaacaaga cataaaaagt aaggcttttt
    60901 aattaaggca ttggctataa atgcgtatta caagcagcga aacggtataa ccactagggt
    60961 tataccgttt cgctatttta ataaatataa aaattttctt tattatttgc ttactatatc
    61021 aatataataa ttttattata ctatggatat agtatgtgtc tttacaagtt gtaaactgac
    61081 agtggtttat tttttaatat aaatattgac tttgatgcag gtaaactttg tatttttaag
    61141 cgtattatgg aatatgttaa ataaaaaaat gatgaaatat agtattgtaa atgccaaaga
    61201 tgcaaaacaa acttaaaaca tttattttat tgttaagtaa tgctataata taatgtgatt
    61261 ttaataatga tagtggaggt ttaaatatga gagtcgaggc ccctataaaa gtagatcgaa
    61321 aaaccaaaaa acttgctaaa agagttgaaa gtggggaaat agcagttata aatcatatag
    61381 acatagatga agttgctgca aactctttag tagaagctaa aataaaactt gtcataaatg
    61441 cggctccttc tataagtggt aggtatccca ataaaggtcc agggatatta actgaaaata
    61501 atatactaat aattgataat gttggagaag agttatttga agaattaaaa gaaggagaaa
    61561 ctatagaagt tgtagatgga aagatatata gaaaaggaaa atttttaggt gcaggagaag
    61621 ttttagacaa gtatgaggtt tcacaccaaa taaaggctgc ctatgagaat ttagctgtag
    61681 aattggatag atttatagat aatactatag attatgctaa aaaagaaaaa ggatttatac
    61741 ttggagaagt agaaattcca aaagtgaaaa ctaattatgc taataaacat gtattgatag
    61801 ttgtaagagg tcaggactac aaagaagatt taagtactat gctttcttat atagaagagg
    61861 taaagccagt attggttggt gttgatggag gagcagacgc tttaattgag tttggatata
    61921 ctccagatgt gattgtaggt gatatggata gtgtaagtga tgaagcttta aaaaaggcta
    61981 gtgaaatagt agtacatgct tatacagatg gaagagcacc aggtcttaag agggttgagg
    62041 aattaggctt agatgcagtt gtatttcctg cgccaggaac tagtgaagat atagctatgc
    62101 ttatcgccta tgagtataag gctgagttaa tagtagcagt tggtacacat tcaaatatga
    62161 ttgattttct tgaaaaagga agaaaaggta tggcaagtac ttttttagtt agactaaaaa
    62221 taggttcaaa gcttatagat gctaaaggcg ttaatttgtt atacagaagt aaattaaaaa
    62281 taaagtacat atgggcatta atagcaacag cattatttcc tgtgttggta gttgcatcat
    62341 tgtcaccagg agttcaacag ttcatacaac taatgcaatt aaagtttaga gtattattac
    62401 agatgtaaat tttaggagga taaatatgca tattaatatg aaatattata tagtcactat
    62461 aggggctata tttatagctt tgggtatagg catgttagta ggatttaatt taaataataa
    62521 tcaacaatta agtgaacaac aagcaaacat aataaatgat ttggatgata agtttaatat
    62581 cttaaaagaa aaaaatgata aacttgacag tgacttagca agtgtaaata gagattatga
    62641 agaggcagtt aattttataa ataaaaatgt agataagatt ttagctggtt cgctgaatgg
    62701 taaaagtata ggaataatat cggctaatga aaatgacgat tatacaaaaa atatagaaga
    62761 cataataaat aaatctaatg gaagtattgc atttaatata gtattaaaag aaaatataac
    62821 aaatccagaa aagttaaagg aaatgtcaac taaactaggt acagatgtaa aaaatgcaaa
    62881 tgatgcagtt aattatataa ttgacaccct taaaaaggaa gatgcaagtg acattttaac
    62941 atatcttcaa gaactagatg taataaagtt taactctctt ggaaatacat atctaaaata
    63001 tgattctgtt gtaatagctg gtggaaatga tgcaaaagac agtacaaaac agtttgaaaa
    63061 aatagaaaaa tttgtagttt caaaacttaa atcagagaat aaatatttag tagaagttca
    63121 aaatacagga gttaaaactt cttatgtaga actatattct aaaaacaaag ttgcaactat
    63181 agacaatatt gatgaaggta taggaagtat atctctagct attcttctac aacaaggtaa
    63241 tatagttggt aattttggta gattggatac tgcaacttca ttgttgccat ctattaaata
    63301 gaaataattt taaggagaga aaaaatggat tcttatataa gtataataat accagcttat
    63361 aatgaagaaa gtagaataaa atatacatta gaaagcatat tggacattaa gcaaataaat
    63421 gaaataatag ttgtggatga tggttcaatt gacaatactg ctaaggttgt atctgagcta
    63481 gaaaatgaaa agattaaatt ttttaagtta gataaaaata gaggtaaagg atatgcttta
    63541 aattatggta tcaaattagc aatgaaaaat gcagatatta taggttttct ggatggagat
    63601 ttaggaagct ctgctaaaga agttgaaaaa ttaatagtac ctatattaaa tggtgaggcg
    63661 gatgttacaa tagccaagtt tcctcctgca aagagaaaag gaggtttggg atttgtaaaa
    63721 ggtttagcta aaaattctgt gtttgagatg actggtgttg agttagatgc aactttatca
    63781 ggtcaaagaa tatttaaaag agaagtttta gaaaagtttg atgaaatacc ttttggatat
    63841 ggagtagaag ttggaatgac cattgatata ttaaagtatg gatttaaaat taaagaagta
    63901 ttagtaaata tgactcataa tgaaacagga agaaatttaa aaggttttat tcatagaggg
    63961 aaacagtatt atcacataaa aaaggtttta ggacaaaaaa agaaagaatg gaggtaatat
    64021 cttagtgaaa gattatatac tttataccat tttattatta actggattat ttggaaccta
    64081 tgctgtaatt ccactattta aaaatttatt gataaatgga aatgtactta gaccaaacta
    64141 taaaaaagat atgataccag taagtatggg aattgtattt ttgcctatga ttgtaatcaa
    64201 tggaataata cttgctttct ttactacaga atttaaggat ttagcgtata tatttatgtt
    64261 tatgtttggt atgatatcca tgttttttgc agggatacta gatgatgtca ttggaaatag
    64321 agatgtaagt ggtttaaagg ggcattttaa aagtttattt aaaggaagtc ttacaacagg
    64381 aggttttaaa gctttatttg gtggatttgt agggcttata gtttctgttg ctatatcaaa
    64441 agacataatt gatataatag taaatacact aataatagct ctatcaacta atttaatgaa
    64501 tcttttggat ttaagaccag gaagggcaat aaaagcatat ttattcataa tggtaattat
    64561 atttttaaca ttgacaggat ttgttcaagt tcttccatta cttatagtac caaatgtact
    64621 tgcatacttt aattatgatt taaaagcaag ggctatgatg ggagatactg gttcaaacgt
    64681 ccttggaata tctattggaa tgctcatttc ttttgggtat ccatttaaca ttagattagg
    64741 atggttaata tttgtagtta ttatgcatat attcactgag aaatactcat taactaagat
    64801 aatagaaaaa aataaactat taaattttat agataaattg ggaaggtagt attatgataa
    64861 gcaaaagagt ggggattgtt gaatcaatag taagtcaaac tgaaactcta gatgatataa
    64921 gagtaaatat aaatggaaaa atacaaaggg catataatta tcccaaaata tctggaacta
    64981 taaatatagg tgatgaagtg gttttaaata caacagcagt agaactgagc ttaggtacag
    65041 gtggatacca ttttgtaata actaatttaa ataatataga atctacttta actgaaggtg
    65101 ggcatataat gaaacttaga tatacacctc ttcaaataaa agtggattct gttgaggaac
    65161 aagaaagtat ttatcatgat aaatttgcga attttaaagg attagaaggt ctttctgttg
    65221 tagtagggac tcttcatagt atgttaactc catttgcagc ttcatttaaa agaaataatc
    65281 caaataaaaa attagtatat ataatgactg atggagcatc tcttccaatt tatttaagta
    65341 aaaatgtgga cactttaaaa gaaaaaaaat taatagatag tacgataact ataggaaatg
    65401 cgtttggtgg agattatgaa tgtataaata tatatactgc attaattaca gcaaaagaaa
    65461 tactaaaagc ggatgcagta tttgtcagta tgggacctgg aattgctggg acaggaacaa
    65521 aatatggatt tacagggatt gaacaaggct ctattcttga tgccgtaaaa aaacttgaag
    65581 gaagagcaat tgcaattcca agaattagtt ttgctgacaa acgagaaaga catcaaggta
    65641 tttcacatca tagtatgact gtatttaaag aaattgtaaa tgtaaatgta gacataccaa
    65701 taactatata tgatgatgaa aaattgaact ttataaaaga gcaaatcaat ttaaatggtt
    65761 tagacaaaaa gcataatata atttatatag ataataataa aacgaaagag gatttagact
    65821 attttaatct aaaagtaaag agtatgggaa gaaattatga acaagatgag gcatttttca
    65881 aagctgcaag cacagcagca tattacttaa tggaggttta agatggtatt agaagaaaaa
    65941 actattagta gtgatagggt ttatacaggt aaagtaatta ctttaaaggt tgatactgtt
    66001 gaaattccag ggcaaggata tcaaaaaagg gagttagtag aagttggtgg agcagtagga
    66061 atagttgcaa tcacagatga taataaagtt gtactagtta agcaatttag aaaacctata
    66121 gaaaagccaa tctttgaaat tccagctggt aaactagaaa aaaatgaaag tccaaaagaa
    66181 tgtgctgaaa gagaattaaa agaagaaact gggtatagtg caaaaaatat taagctaata
    66241 cataaatttt tcacttcagc aggtttttct aatgagataa tgtttgtata tttagcaaca
    66301 ggtcttactc caggagagaa taatttagat gcagatgagt ttttagatgt ctatgaaatt
    66361 gagcttgaag aagcatataa catggtatta aaaaatgacg ttgaagatgc aaaaacatct
    66421 attggattat tattagtaaa agatatgttt aaaaattaat atttataaaa tgaataaccc
    66481 tttgtcctaa agcatattat tttataaagg acaaggggga attaaattga gaagaacata
    66541 tagaaaaaat gattttagag aattaaatag acaaataatt attataggat tgttgtttat
    66601 gatgtctatt gttgttgggt cttacataaa taaaatattg ccaggttcta gtaacaatat
    66661 attaaacaat ataaatcctg ctgtagagta ttataatcta aatataagca taaaagatac
    66721 agtgatacaa aatttaaaat cagatgcaat ctttatgggc tcaatagctc tattaagtct
    66781 atttgtagta acaattccag cagtgttggt agcttttgta ttaaaaggga tgtcaattgg
    66841 ttatacaata aatagttgta tattggcatt aaaatttaaa agtataaaaa tgattttaat
    66901 aatattattt aaaaatttga ttattattcc atgtgcaatc atattagcat taattagttt
    66961 aagttatttt aaagaaatgg tttatgaatt taaaaagaaa aatagaaaaa atatgcaatt
    67021 tttaattaag aggtatatac taaatattat aattataata gcactatcat tggggttgca
    67081 attaatatta aatactgtaa gtattggtat tattaagttc ctagctaaat aaaggagttt
    67141 ataatggata ttatagaggg atatatagat tacataaaag ataaaaaaaa attatcagag
    67201 aacacagtgg catcatactt tatggatata aaaaaatata tggaatatat aaatcaaaaa
    67261 agcattaaac tagtagatat agtagaaaat gatattataa gttacttaat aaagttggaa
    67321 aaagataatg tatcaatagc tacaatagcg aggatgatat cctctataaa atcttttcat
    67381 gattatttat ttttaaatca tatatgtaca aataatcctg caaaggatat aaaaaaacct
    67441 aaaataaaaa aagaaaatat aaatatactt acagaagaag aaattgaaag actattaaat
    67501 tttccaaagt taacaacacc taaattgatt agggacaagg ctatttttga agtattatat
    67561 ggtactggta taaaagtatc tgaattagta gagatgaata tagaggatat tgacctagat
    67621 attgattata tatattgtaa ttcatgttgt aaaaatcaaa gagtgatacc actttgtgat
    67681 attacaaaac tttacttaga aaagtatcta aaagaagcaa gacctaaaat ggctgtagaa
    67741 ggtgaaaaat cattattcgt aagttcatta ggtcaaaggt ttacacgaca aggtctttgg
    67801 aaagtcataa aaaaatattc taatctagcc aatatagata aaaatataaa tcctacaatg
    67861 cttagacatt catttgctat tcatttgtta aatgaaggtg ctaacattgc tgttgtaagt
    67921 aaaattttag gaaatgttaa tttatctagt ttgcaagtat atttaaatca tatagataaa
    67981 aacgtgagaa gagaaataaa ggaaaaacat cctagaaatg atgttgatgt agaattacaa
    68041 aaagcagaag ctaaatagac tatatagcaa attttataat ttaaaaggag tatatttata
    68101 tgagcagagt aatttggatt gtaattgata gtgttgggat aggagcatta cctgatgctg
    68161 aaaaatttgg tgatagtaag gatgtaagta cccttggaaa tatatttaag gaatatccag
    68221 atattcagat tcctaatatg agaaatttag gtataggaaa catagatggt atagattttt
    68281 ttgaaagtat aaaagaacca ataggttgtt ttggaaagtg taaggaaatg tctcagggaa
    68341 aagatactac tacaggtcat tgggaaatga ctggtataat agttgacaaa ccatttaaga
    68401 catttgaaca tggattctct aaggagataa tagaagagtt tgagaaaaaa actggaagaa
    68461 aagtagtagg aaataaacca gcttcaggaa ctgtaataat agatgagtat ggagagcatc
    68521 aaataaaaac tggagatgta atagtatata catcagctga tagtgtattt cagattgctg
    68581 ctaatgaaga agtaattccc ctagaagaac tctataatat gtgcaaaatc gctagggaaa
    68641 taatgatggg ggataatgca gtagctagag taattgctag accattcata ggtaagaaaa
    68701 aaggtgagtt tgtgaggact tcaaatagaa gagattattc actagaccca tttgaaccaa
    68761 cagttcttga taatataaaa gaatcaggac ttgatgtttt agcagttgga aaaatagaag
    68821 atatttttaa tggaaaagga attacagatg ctatccacac taaaagcaat atggatggag
    68881 tggatgaaac tttaaattat atgaaacaag ataataaagg tctaatatat tcaaatctcg
    68941 ttgattttga ttctaaatat ggtcatagaa gagacccaga aggatataag aaagctcttg
    69001 aagagtttga tagtagactt cctgaaataa tggctaatat gagagaagat gatattttaa
    69061 taatcaatgc agaccatgga aatgacccaa catataaagg tacagaccat acaagagaat
    69121 atatacctgt tatgatttat ggcaataaga ttaaaaaagg ttttaattta ggagtaaaag
    69181 atacttttgc tgatattgga gcaactgttg cagatatact aaatgtaaag ttgcctaaac
    69241 atgggtcaag ctttaaagaa gatttatttt aaactataaa atgataaaaa cttagcttaa
    69301 taggatggag agattggaat gtttgaaaag atagcacaaa gtagtaaatt tataaattct
    69361 aaaagtaata taaaacctaa gattggtttg atattaggtt ctggactagg tgatttagca
    69421 aatgatatag aagaccctgt cacaattaaa tatagtgaaa ttccaaactt ccctgtatca
    69481 acagtagcag gacatgctgg acaacttgta attggaaaac ttgaaggaaa agaagttatt
    69541 gctatgcaag gtagatttca ttactatgaa ggatattctc agaaagaagc tacttttcca
    69601 gtaagggtta tgaaagaatt aggagtcgag attcttatag taactaacgc tgctggaggc
    69661 gtaaataaag aatttaaagc aggagatttg atgcttataa gagaccatat aaactttagt
    69721 ggaagtaatc cattagtagg aaaaaatgat gacagatttg gtgctagatt tccagatatg
    69781 tcagatgcat attcttctaa atatgtagat gtagttaaaa actgcgctaa agaatgtaat
    69841 atagatgtca aagaaggcgt ttatatgttt tttagtggtc caaactatga aacaccagca
    69901 gaagtgagaa tggcacaaat tttgggagct gatgctgtag gtatgtctac tgttccagaa
    69961 gtcatagtag cttctcactc caatatagga gttattggta tatcttgtat aacaaatatg
    70021 gctgctggca tcttaaatca accactaagt catgaggaag taatagaaac tactcaaaaa
    70081 gttaaatcag aatttatgaa tttagttaaa acaacggtta aaaatttata agttaaagct
    70141 tattatatat tttttgagga agtttataat aaaatagtta atgtttaatt tgaaaagaat
    70201 taataaaaag ctgtcttgat atagaaagtt atttctaata aaggacagtt tttttataat
    70261 actacaacat ttgaaagtgt tttaaagttt aatatttatt taaattatta tagattagaa
    70321 tggagatttc aattatgaga atgtatgata ttataaaaaa aaagagagat aacttagagc
    70381 ttagtaaaaa agagataaac tactttatag aaaactattc aaagggagat atacctgact
    70441 atcaagcttc tgcactatta atgtcgattt atttaaataa gatgaataaa caggaaactg
    70501 tacatcttac agaagcaatg atgaactctg gagacatgat aaacctgtct gaggtagatg
    70561 gaataaaggt agataagcat agtactggtg gtgttggaga taagactacc atagctctta
    70621 tacctttggt tgcttcatgt ggagcaccag ttgcaaagat gtcaggtaga ggtcttggtc
    70681 atactggtgg tactattgat aaattggagt ctataccagg tttttcgaca gaaatggaga
    70741 taagtaaatt tattgattct gtaaataagt ctaaaatagc agtttgtggt caaagtgcaa
    70801 aggtagcaat tgcagataag aaaatatatg ctcttagaga tgttacagca actgttgata
    70861 atatatcatt gatatcatca agtgtaatgt ctaaaaagtt agcttcagga gctgatgcaa
    70921 ttgtgttaga tgtaaaaact ggtgatggag catttatgaa gacattagaa gagtcttttg
    70981 agttagctaa atctatggtt gatataggta ctggtatgaa tagagataca atagggattg
    71041 taacagatat ggatgaacct ttaggttttg cagtgggaaa ctctttagaa gttatagaag
    71101 caattgagac attaaaagga aatggcccta aagaccttgt tgagctatgt gaagtattag
    71161 gagcatatat gttggtttta tcaaaggtag cacatgattt tgaagatggt gttgaaaaga
    71221 taagagaatc aataaaatct ggtactgcta tacaaaaact caaagagttt atagagaatc
    71281 aaggtggtaa taaagatgta gtagatgatt attctttatt caaaaaatca aagtataaat
    71341 atccagttaa atcaattaaa tcaggttata taagtaaaat taaagctgaa gatgttggtg
    71401 tatcagctat gattcttgga gcaggtagag aaactaaaga tgatatttta gatttatcag
    71461 cagggataat tttagaaaag aaagtaggag attatgtaaa tgaaggtgaa gttttagctt
    71521 acatgtatta taatgatgaa caaaagcttt cattggcaaa gggaagattt attgattcct
    71581 actctattgt agattcaaaa atagaaaaaa ataaattaat atatggtatt gtaactaaag
    71641 aagagattaa aaagttttag aaaattttag aaaagtgatt ataaaaggcc taacatgtca
    71701 aaaataatgt taggtttttt attttttgat aaactataaa ttataccaaa aatatatatt
    71761 ttaaaacatt ttttacaata taaataagtt tattctacaa gatgattgaa aattaaagta
    71821 tctagtttta taatagaaaa tggataaata tacataagga gtagattata ttatgaactt
    71881 taaagaaaat agattgaaaa tagcagtatt aataattgtt attttgatta tcttagcagg
    71941 tatatttgtg tttatacaaa taggtcctta tgataaaaat aacaaaaaag atgtaattat
    72001 tgacgttcca agtggagcat ctgttggaaa aatatctgac atattatatg aaaataaatt
    72061 aataaagaat gaactattat ttaaactttt ggtaaaggtt agcaataaag ctccgtctat
    72121 aaaatcagga acatacttac ttaatcaatc gtattcaaac aatgatataa taagtctttt
    72181 agtttcaggt aaaatatatc aggatggtat aaaagtgact attccagagg gagctacatc
    72241 taaagaaatt atagcaatgt tagtaagtaa aaatttagga gataaagcta cttttgaaaa
    72301 tttaataaaa aaacctcaag aattttatga taaatttcca tatttgaagg aagatggaat
    72361 aacctcttta gaagggttct tgtatccaga aacttattat tttaattcaa agaaacaatc
    72421 agaagaagat attttaagcg aaatgttaaa agtatttgac agtaaataca cggacaagtt
    72481 taaaaagaaa caaaaagaat taaatatgac tcttcaagaa gtaatggaaa tggcatcaat
    72541 aattgagaaa gaagctgttc ttgacaaaga cagacctata atagcaagtg tgttttataa
    72601 tagacttaag gtaggtatgc ctcttcaatc agatgctaca atacaatata tatttgaaga
    72661 gagaaagaaa attgtaacat atgatgattt aaaaattgat tcaccatata atagttataa
    72721 aaataaggga ttacctccta caccaatatc aaatcctggt ataaaatcta ttgaagcagc
    72781 attatatcca gagaaaacag actatttata ttttgtagct aagattgatg gtggaaataa
    72841 ttatagtaca aactatcaag accatttaaa atacgttaaa gaatataaag aagcaaggga
    72901 taaacaatca aaagatacaa aagcaacaaa taaagaaaat acaaaaaaat aaaccatgat
    72961 tataaaaagt ctaacagggt aagaataatg ttgggctttt ttattgaaaa ttgttattct
    73021 tagatttata ataaagaaag tgtaaatata tataacaata tgaatttaaa aaataataaa
    73081 ttaatataaa acttgaatat ttattatttt agggagtgtt tttatgagta atatagttaa
    73141 tgatttggtt gaagattata taagagtgac attaaaagaa aaagaaggat ttttaaaaga
    73201 tttagaaatt tatgcagagg aaaatagtgt tcctataata cataaagaag tttcagattt
    73261 attaaaagtc ttgttaaagg ttcaaaaacc aaagagaatt ttagaagtag gatgtgcaat
    73321 tggttattct tctatttttt ttgcaagtat tataggaaaa gatgcagata taataactgt
    73381 tgagagaaat gaaaaaatga tagaaaaagc aaaagaaaac ataaaattag ctggatttga
    73441 caataacata actatactag aaggagatgc agaagaaaaa cttagtcaag tccaaggtga
    73501 atttgacctt atatttatag atgcagccaa aggtcaatat aagctatttt ttgatttagt
    73561 tatagataaa ttaaaagatg ggggactttt agtatctgat aatattttgt ataaaggtat
    73621 ggttgctcat gatgattttg ttgtaagaag aaaaaagact atagttaaaa gaatgagaaa
    73681 ttatttagat tatatatgta attgtgacta tttaagcaca tcattggtac caataggtga
    73741 tggtgtagca ctaagttata aagaaagtaa aagaggtgat gtagatggaa ttaaataaag
    73801 tagaactttt agctccagca ggtgatttgg agagattgaa aatagctata acatatggag
    73861 cagatgccgt ttatataggt ggagaaattt ttggaatgag gtctgcagcg aagaatttta
    73921 gtaaggaaga tatggcagag ggagtagctt ttgcacatga aagaggcaag aaagtattcg
    73981 taacagtaaa tataatacct cataatgaag attttctaca attagaagac tatttgttgg
    74041 aactagaaga gataggaata gatgcagtta taatagctga cccaggagtg cttagtgtca
    74101 ttaaaaaagt aattccgaat atggaaatac atttaagtac acaagcaaat acaaccaatt
    74161 atctaagtgc aaacttctgg tacgagcatg gaataaaaag agttgtagtg gcaagagaat
    74221 tatcttttga tgaaatatct gagataagag caaaaacacc tttagatatg gatatagaag
    74281 cgtttatgca tggtgctatg tgtatttctt attctggtag atgtttaata agcaactata
    74341 tgactggaag agatgcaaac aaaggttctt gtgctcaatc ttgtagatgg caatatcatt
    74401 tggtggaaga aaaaagacct ggtgagtatt ttccaatata cgaagatgaa aggggtacat
    74461 tcttctttaa ctcaaaagat ttatgtatga tagagtatat accagagtta atcaaatctg
    74521 gtataacaag tttaaaaata gaaggtagaa tgaagactgc atactatgta gctactgttg
    74581 taagagcata cagaatggca atagatgaat tttacagagc cccagaaaac tggaagttca
    74641 atccaatgtg gatggaagaa ctaaaaaaag gtagtcatag acattttaca tcaggttttt
    74701 acttgaataa accaacaaca gaagaccaaa attatcaatc agcatcatat gttagaaact
    74761 atgattttat aggtatagtt agagaaacag aagatgaaga tggtcttatt gttgttgagc
    74821 aaagaaataa aatgtgtgtt ggtgatgaaa tagaagttat gggaccatat aaagaaacta
    74881 tgtttactaa aattgaagaa atgtacaatg aagaaggaga agctatagaa tcagctcctc
    74941 atcctagaca aatagtgaag ttaaagctat cagtaaaagt tggtaaagat tatatgctta
    75001 gaaaagttat agaagaaaag gttgaagaat aataaaaaat tgataaaaat agtattagcc
    75061 ccttagaaat tgtcaataat gaaattatct aaggggtgat ttcatgtcaa aaaagaagac
    75121 tccatttttg aaaaaagtgg gaaagagaag ctggtgtata tttactataa ttttaataat
    75181 ttatagtgtt ttaatttata ggctagttga tatacaagta ttaaaaggtg ataaatacaa
    75241 acaaagtgtt gaatcacaaa gcgttgagaa agtcgagtta aatagtggaa gaggaataat
    75301 ctatgataga aacaacaaga agcttacaga tacaagtaaa tctcaggtat taattgttga
    75361 aaaagaaaaa ttaaataata actataaaat tttggaactt attaagaaag ctactaagat
    75421 gaatgattta gatatatata aagctgtaca agagcaattg acacgcccta taatacagat
    75481 tcagactaaa aatatcgata agagtatgaa aaaagaactt gagaaaaatg gaataatggt
    75541 agaagaaaaa actatgaggt acgcaaaaga tggtttatta tcacatacaa taggatatat
    75601 aaaagaagat gataaatcag gtcaatcagg aattgaaaag agtatggata gtgtacttag
    75661 aaattcaaat gaaaagtata taagtgcttt taaagctgga gatgcaggaa atgaaaaatc
    75721 tctaaacata ctaaagggaa gtgtaaaaac tgttgataat aaggataaag atagacatct
    75781 aaaaactact attgattata acattcaaaa aaaattagaa caaatactaa ataaagaaga
    75841 aaatcctaca gcagcaataa tctcggaagc atctacaggt gaaattcttg caatgtgctc
    75901 tagacctaat tttgaccaaa atgacatatc aaaaagttta aaaggaaaaa atggggaatt
    75961 tgaaaataga gttataaaag cgacatatcc accagggtct gtatttaaga tggtagtttt
    76021 attttctgca cttgaaaatg gtgttataga tgagaactat acttacaact gtacaggtaa
    76081 gactaaagtt gggaatacta atgaaatact gcgatgtaat aaaagagatg gtcatggttt
    76141 ccaaaactta agacaagctt tttctaattc atgcaatcct gcatttttag atatagctat
    76201 gaaacttgga aaagaaaaga tactcaaatc tgctgaaaaa ttacatttat ttgaaaaggt
    76261 tgatattggt cttgatgaag aaaaaattag agaagctcca aaaaacatat caataagaaa
    76321 tttagctata ggtcaagaaa atatagaatt tacaccactt caaataaatc aaatgactca
    76381 aataatagct aataatggaa cattcaaacc actatactta tataaaagtc ttgtggacaa
    76441 taatatgaat acaataaaaa cttataagtc ttcaaaaaaa gaagaactta tttctcctta
    76501 tgtgtgtaca caagttaaag aatatatgaa gtctgtatct aggattggta cagctaaaga
    76561 cctaaaagat attgaaggtg gttgtggagt taaaactggt acagctcaaa gtagtttaaa
    76621 taaaaaagct atagaccacg gatggataac aggattttat cctgaagaaa gacctaagta
    76681 tgtaataact gtgttagtag aaggaactca aaaaggaaat aaatctgcaa cacctatatt
    76741 taaggaaata tgtgaaagta taaaataaat tatgtactat aaaatttaag gatatatcgt
    76801 aactttgatt ttagatagaa aactttgtct aatgatgtac gatatatcct tttaaaatat
    76861 agtattaact ttcttgaaca agctcttttt ctagtttttt aagagctttt ttttcgattc
    76921 tagatacata tgaccttgat atatcgagtt tttgtgcaat ttctctttgt gtcttatagc
    76981 cataaggagt taacccatat cttaactgga caatttctct ttctctagga gtaagaattt
    77041 tgtttagttg ttcgtaaagt tttccaactt gaacttttag ttcaacttca tcaagaacat
    77101 agttagcctc tgtgccaagt atat
//
LOCUS       DAELXX010000004        76916 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.004, whole genome shotgun sequence.
ACCESSION   DAELXX010000004 DAELXX010000000
VERSION     DAELXX010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 76916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76916
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.004"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            75..1376
                     /locus_tag="KPH29_000224"
     CDS             75..1376
                     /locus_tag="KPH29_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HBG1465869.1"
                     /translation="MGVIIIQIRRPLLIILMFVVAISFIITNNTKNDEELNDKIITIQ
                     GVVKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGR
                     YIKSTGYKGIVYINNYKIIDKNKLYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEV
                     ENLTEEQKEIFTRTGTSHVISISGLHTGILCVIISFLLRGINKLYKLLILSIFITLYC
                     IMVGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGIILIMNNPYVIYNVSFQLS
                     FLATLSILIFYNKINSIIKLSMVSLTISSNILTLPIIYYTFKGIPLLSIIGNLIIVPF
                     VGVIMYLSIASLIVFKVSVVIAKIISFFNSTLIESIFFLLEKISNLSFAYINIESPKF
                     YIVVIYYIGVFFYIFYIEGKEIKEQENELQGYYKECKREKL"
     gene            1330..2373
                     /gene="holA"
                     /locus_tag="KPH29_000225"
     CDS             1330..2373
                     /gene="holA"
                     /locus_tag="KPH29_000225"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBG1465870.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(2560..2826)
                     /gene="rpsT"
                     /locus_tag="KPH29_000226"
     CDS             complement(2560..2826)
                     /gene="rpsT"
                     /locus_tag="KPH29_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBG1465871.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
     gene            3425..3946
                     /locus_tag="KPH29_000227"
     CDS             3425..3946
                     /locus_tag="KPH29_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1465872.1"
                     /translation="MDIAKLILNPARLRILQYIRLHGSARTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVVDNDKTK"
     gene            3980..4756
                     /locus_tag="KPH29_000228"
     CDS             3980..4756
                     /locus_tag="KPH29_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG1465873.1"
                     /translation="MKFHEFGDKNKPHIMLIHGGGNAWWNYLRQAEVLSNNYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDEI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKM
                     FQSYVPSCEIYEAKGYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            5099..6052
                     /locus_tag="KPH29_000229"
     CDS             5099..6052
                     /locus_tag="KPH29_000229"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HBG1465874.1"
                     /translation="MINVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            6070..7089
                     /locus_tag="KPH29_000230"
     CDS             6070..7089
                     /locus_tag="KPH29_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HBG1465875.1"
                     /translation="MFKKCIKVVTLTFILACILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEENTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            7120..7371
                     /locus_tag="KPH29_000231"
     CDS             7120..7371
                     /locus_tag="KPH29_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1465876.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIIGTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            7653..9458
                     /gene="lepA"
                     /locus_tag="KPH29_000232"
     CDS             7653..9458
                     /gene="lepA"
                     /locus_tag="KPH29_000232"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBG1465877.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDERRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            9609..10946
                     /locus_tag="KPH29_000233"
     CDS             9609..10946
                     /locus_tag="KPH29_000233"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBG1465878.1"
                     /translation="MINIEIPKKVDYIIKELETNGYEAYVVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLISTEVIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILRRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVELFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGHILNKMLEIVINNPELNEKEILKEKALNIYTF"
     gene            11179..12492
                     /locus_tag="KPH29_000234"
     CDS             11179..12492
                     /locus_tag="KPH29_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG1465879.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTIISLGGIFITLLGLPVYYYMKKR"
     gene            12664..13839
                     /locus_tag="KPH29_000235"
     CDS             12664..13839
                     /locus_tag="KPH29_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBG1465880.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMKLY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINVDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            13942..14961
                     /gene="hrcA"
                     /locus_tag="KPH29_000236"
     CDS             13942..14961
                     /gene="hrcA"
                     /locus_tag="KPH29_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HBG1465881.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            14991..15611
                     /gene="grpE"
                     /locus_tag="KPH29_000237"
     CDS             14991..15611
                     /gene="grpE"
                     /locus_tag="KPH29_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBG1465882.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            15703..17550
                     /gene="dnaK"
                     /locus_tag="KPH29_000238"
     CDS             15703..17550
                     /gene="dnaK"
                     /locus_tag="KPH29_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBG1465883.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLADEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSEAEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            17761..18915
                     /gene="dnaJ"
                     /locus_tag="KPH29_000239"
     CDS             17761..18915
                     /gene="dnaJ"
                     /locus_tag="KPH29_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBG1465884.1"
                     /translation="MSTKRDYYEVLGISKDAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRARRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            19881..20816
                     /locus_tag="KPH29_000240"
     CDS             19881..20816
                     /locus_tag="KPH29_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBG1465885.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKRRFPD
                     AEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKDYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EDLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            20852..22225
                     /locus_tag="KPH29_000241"
     CDS             20852..22225
                     /locus_tag="KPH29_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG1465886.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEGNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(22413..23180)
                     /locus_tag="KPH29_000242"
     CDS             complement(22413..23180)
                     /locus_tag="KPH29_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1465887.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNAIYVIDF
                     LDKNNLDNLKGNFYLIYQSHIDFEYLKNIFYNLFEDINIIQHNGFFIVNSKYNLDINV
                     TTQNIIETETYQSTYIFYLGELDSKADFYFRLQLCSDLLPHIIKDNAENKFLNLFDLI
                     RYKTLDLINEDNILNKLIDFNKIKSIDEELLYTGIKFINNDLNISKTSTSMFLHRNTL
                     VYRLEKINEILGFDLKNFENAMIFYLSVKSYFLYKKI"
     gene            complement(23210..24319)
                     /gene="ugpC"
                     /locus_tag="KPH29_000243"
     CDS             complement(23210..24319)
                     /gene="ugpC"
                     /locus_tag="KPH29_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBG1465888.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNDIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRKLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDKIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
     gene            24578..25315
                     /gene="cas6"
                     /locus_tag="KPH29_000244"
     CDS             24578..25315
                     /gene="cas6"
                     /locus_tag="KPH29_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBG1465889.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGEERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESIRGSGLRKPLEFIPLNFKKRVLKEKIRGFKVREYYYINAYAGTFFLKGN
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            25320..26795
                     /locus_tag="KPH29_000245"
     CDS             25320..26795
                     /locus_tag="KPH29_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1465890.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFYELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENQLFKVFKNGKKETLEKIFSNIDNLTYVNFI
                     EELDKSSATEKQKEGIKKDFKNRRFPYVRNSGKYGLNTSLENMETNVKRIVDTVIKSN
                     IEIDEIDKLDLTQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKR
                     SGQVESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKS
                     FYKKLLHEKISSIRLYRFDIDTNKGIILKFDSIIEYKELLKKIELIDIIDNYNFSREP
                     GNTRNLGKKMIDNGNLSNLKELLLDNISVLRQLTGTSREMDFVSSSYNIGLYIKLCWI
                     VDGGEEYKMKNYMESNLIYSRVGKDLFNKMTDESRRKNFSMKVIQLLKSNDRTQLFQT
                     IMHVLVSNGILIGEGFVEGLMQSSELELNTNVGLFIQEIMK"
     gene            26815..27828
                     /locus_tag="KPH29_000246"
     CDS             26815..27828
                     /locus_tag="KPH29_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HBG1465891.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYREKIHA
                     KTSIATITYDLRRMLHQEYGWNLSNIIFGKKGNIYPSIKQNVGCVDENGLETDVFGYL
                     IPLKDKGSISKASPLRIIPFRSLNSYRGSTQLITNRGFLSSEFGRKYYDEKEENEVLR
                     DENFPTSQALAIEETLSDYYVYTITLELDRIGVVEVEDGKLLLPEERKFMSKELREKA
                     VKDILDAITVFTRNIKHSSVLLKPLAVMGGAFDKVVPFFWDDVDYNADSGEINLEGVI
                     ETIESYSLKESNTILAINDKLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMWY
                     LKE"
     gene            27844..28650
                     /gene="cas5"
                     /locus_tag="KPH29_000247"
     CDS             27844..28650
                     /gene="cas5"
                     /locus_tag="KPH29_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HBG1465892.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYTNMRKGKDKEKFLLQGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIKKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVELKEVMPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPY
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCNVCIDTKEVFTWL"
     gene            28677..31085
                     /locus_tag="KPH29_000248"
     CDS             28677..31085
                     /locus_tag="KPH29_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBG1465893.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKDILEKMNLLEEDKLYIYKSIMLHHGNYEDYMRLSTSKVQEEIYDYIE
                     KGILEKEEFNLKDIESYINDILNVDFKFGEDVLDYDYIDKLSESMFIDSDYNNGQIDD
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDDMILNEIYKSQGNLE
                     KNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIIFQEESNENVTDKGEIVFIESNKEEEICEGKDVKVS
                     FIKEKEIKSIVEGALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRF
                     KFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKNTTKNILKDILKTNSIFTMGIRKTILDDYYDNSVVK
                     NYFEENFISCDKEIKNIYGINKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDVDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            31526..32473
                     /gene="prmA"
                     /locus_tag="KPH29_000249"
     CDS             31526..32473
                     /gene="prmA"
                     /locus_tag="KPH29_000249"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBG1465894.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQDGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            32492..33250
                     /locus_tag="KPH29_000250"
     CDS             32492..33250
                     /locus_tag="KPH29_000250"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBG1465895.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEMILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNNVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            33252..34550
                     /gene="mtaB"
                     /locus_tag="KPH29_000251"
     CDS             33252..34550
                     /gene="mtaB"
                     /locus_tag="KPH29_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HBG1465896.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            34593..34943
                     /locus_tag="KPH29_000252"
     CDS             34593..34943
                     /locus_tag="KPH29_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HBG1465897.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            35106..35285
                     /locus_tag="KPH29_000253"
     CDS             35106..35285
                     /locus_tag="KPH29_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBG1465898.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            35315..35758
                     /locus_tag="KPH29_000254"
     CDS             35315..35758
                     /locus_tag="KPH29_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBG1465899.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            35869..36366
                     /locus_tag="KPH29_000255"
     CDS             35869..36366
                     /locus_tag="KPH29_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBG1465900.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            36559..37122
                     /gene="raiA"
                     /locus_tag="KPH29_000256"
     CDS             36559..37122
                     /gene="raiA"
                     /locus_tag="KPH29_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBG1465901.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
     gene            37335..37571
                     /locus_tag="KPH29_000257"
     CDS             37335..37571
                     /locus_tag="KPH29_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HBG1465902.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            37584..38927
                     /gene="yqfD"
                     /locus_tag="KPH29_000258"
     CDS             37584..38927
                     /gene="yqfD"
                     /locus_tag="KPH29_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HBG1465903.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKEFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            38866..39981
                     /locus_tag="KPH29_000259"
     CDS             38866..39981
                     /locus_tag="KPH29_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBG1465904.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKVKMQEKTFKRK"
     gene            40038..40499
                     /gene="ybeY"
                     /locus_tag="KPH29_000260"
     CDS             40038..40499
                     /gene="ybeY"
                     /locus_tag="KPH29_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBG1465905.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            40505..41224
                     /locus_tag="KPH29_000261"
     CDS             40505..41224
                     /locus_tag="KPH29_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBG1465906.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            41343..41744
                     /locus_tag="KPH29_000262"
     CDS             41343..41744
                     /locus_tag="KPH29_000262"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBG1465907.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            41808..42701
                     /gene="era"
                     /locus_tag="KPH29_000263"
     CDS             41808..42701
                     /gene="era"
                     /locus_tag="KPH29_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBG1465908.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            42713..44092
                     /gene="mgtE"
                     /locus_tag="KPH29_000264"
     CDS             42713..44092
                     /gene="mgtE"
                     /locus_tag="KPH29_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBG1465909.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            44165..44320
                     /locus_tag="KPH29_000265"
     CDS             44165..44320
                     /locus_tag="KPH29_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HBG1465910.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            44286..45059
                     /gene="recO"
                     /locus_tag="KPH29_000266"
     CDS             44286..45059
                     /gene="recO"
                     /locus_tag="KPH29_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBG1465911.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            45052..45651
                     /locus_tag="KPH29_000267"
     CDS             45052..45651
                     /locus_tag="KPH29_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HBG1465912.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            45811..46689
                     /gene="glyQ"
                     /locus_tag="KPH29_000268"
     CDS             45811..46689
                     /gene="glyQ"
                     /locus_tag="KPH29_000268"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBG1465913.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            46689..48755
                     /locus_tag="KPH29_000269"
     CDS             46689..48755
                     /locus_tag="KPH29_000269"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBG1465914.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQSDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVALLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKVLIEHALD
                     NYSELDFNKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIRLYNEFKQVKVKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLEICDL
                     SKIVYK"
     gene            complement(48921..50468)
                     /locus_tag="KPH29_000270"
     CDS             complement(48921..50468)
                     /locus_tag="KPH29_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HBG1465915.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEELFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     VENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNEEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DAFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
     gene            50748..51515
                     /locus_tag="KPH29_000271"
     CDS             50748..51515
                     /locus_tag="KPH29_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBG1465916.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            51798..51995
                     /locus_tag="KPH29_000272"
     CDS             51798..51995
                     /locus_tag="KPH29_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG1465917.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            52024..53100
                     /gene="vorB"
                     /locus_tag="KPH29_000273"
     CDS             52024..53100
                     /gene="vorB"
                     /locus_tag="KPH29_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBG1465918.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            53103..53822
                     /locus_tag="KPH29_000274"
     CDS             53103..53822
                     /locus_tag="KPH29_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBG1465919.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            53835..54374
                     /locus_tag="KPH29_000275"
     CDS             53835..54374
                     /locus_tag="KPH29_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBG1465920.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            54531..55589
                     /gene="buk"
                     /locus_tag="KPH29_000276"
     CDS             54531..55589
                     /gene="buk"
                     /locus_tag="KPH29_000276"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBG1465921.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKEGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            55607..56500
                     /locus_tag="KPH29_000277"
     CDS             55607..56500
                     /locus_tag="KPH29_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBG1465922.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            56530..57738
                     /locus_tag="KPH29_000278"
     CDS             56530..57738
                     /locus_tag="KPH29_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG1465923.1"
                     /translation="MKYAKRMDMVKASAIRASQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDIVVTENPSYLGAINAFRPYECDFLGVDTDDEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            57762..59024
                     /locus_tag="KPH29_000279"
     CDS             57762..59024
                     /locus_tag="KPH29_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG1465924.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFSTPVLGMRWVMISLGILSIVMCPVLWFVIKDVDIREDKEEEASEKMSAGDFM
                     TVLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFG
                     APFGGYCADKVKSPTKVLLPIYVFGIAVIILFLVLPASTPMTIFIALTFVVGILGYMG
                     KGIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIF
                     QIAILIIGILSCLYILKVKKKRESKLEA"
     gene            59600..61063
                     /locus_tag="KPH29_000280"
     CDS             59600..61063
                     /locus_tag="KPH29_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBG1465925.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSMKLRFFQIKDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSILFSVVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVTSSIALGIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIIANINLVPGAIMTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIIITGMYNVTDVYTTLPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKHIIN
                     VCRFMLIICAAMCCFIEPLTSWALGDICIGVLTYINLVGIFTLKKPAIKAYYDYQRQK
                     KMGVKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
     gene            61119..62255
                     /locus_tag="KPH29_000281"
     CDS             61119..62255
                     /locus_tag="KPH29_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG1465926.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            62491..63606
                     /locus_tag="KPH29_000282"
     CDS             62491..63606
                     /locus_tag="KPH29_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HBG1465927.1"
                     /translation="METLEVRNIELSGTNYEIGYRLGELVANMPEIIEGQINKSNLVS
                     KKEEKEMIELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALTPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTVMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKEVNIINSLESKREYFLHSTNHTHIDKLHKLEPQSMKNSIHRYKL
                     IKEYINKAKKIGEKELMNLLSLKYPNGLSCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKNDIKSITQRININIEKAPSDFFDFIE"
     gene            63758..64627
                     /locus_tag="KPH29_000283"
     CDS             63758..64627
                     /locus_tag="KPH29_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG1465928.1"
                     /translation="MDYLKYFEKAVMYIENNLHENITVNDVAKETGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEKIKVIGIKDKVILENNSLPD
                     LWEKFRKVHHIVPNTLPSKRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGSLDNLDKTYEYIWGTWFLSSKEELDIRDDFEVYDERFLGPS
                     NVNSQIDIYIPIK"
     gene            65003..65548
                     /locus_tag="KPH29_000284"
     CDS             65003..65548
                     /locus_tag="KPH29_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBG1465929.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
     gene            65561..66574
                     /locus_tag="KPH29_000285"
     CDS             65561..66574
                     /locus_tag="KPH29_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBG1465930.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            66796..68769
                     /locus_tag="KPH29_000286"
     CDS             66796..68769
                     /locus_tag="KPH29_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBG1465931.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFSNSKYFKGIKKEEIINLEEKLLTLFKENNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     IIYLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILASLEKMMDHLMDLGCIDDKFRKNIVNREEKSPTVFD
                     KGLLFPHSVNEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYE
                     EVLKIGQDVKLTNKISKCRTFLEFKKVLLKNLI"
     gene            68838..69197
                     /locus_tag="KPH29_000287"
     CDS             68838..69197
                     /locus_tag="KPH29_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1465932.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAIISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            69232..69588
                     /locus_tag="KPH29_000288"
     CDS             69232..69588
                     /locus_tag="KPH29_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBG1465933.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            69625..70917
                     /locus_tag="KPH29_000289"
     CDS             69625..70917
                     /locus_tag="KPH29_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBG1465934.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDINLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
     gene            71353..71991
                     /locus_tag="KPH29_000290"
     CDS             71353..71991
                     /locus_tag="KPH29_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1465935.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
     gene            71998..72840
                     /locus_tag="KPH29_000291"
     CDS             71998..72840
                     /locus_tag="KPH29_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBG1465936.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            72901..75528
                     /locus_tag="KPH29_000292"
     CDS             72901..75528
                     /locus_tag="KPH29_000292"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBG1465937.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            75742..75987
                     /locus_tag="KPH29_000293"
     CDS             75742..75987
                     /locus_tag="KPH29_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG1465938.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
     gene            76199..76516
                     /locus_tag="KPH29_000294"
     CDS             76199..76516
                     /locus_tag="KPH29_000294"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG1465939.1"
                     /translation="MSKKHKKYSKELKLKAVNLYIKEGYSSYKIAEMLNIRSKTQVQN
                     WVKDYKNKGKTAFNEETRGRFKNISLENDNRKFKSVEEELKYLRMENEFLKKLSTLLD
                     KSK"
     gene            76543..>76916
                     /locus_tag="KPH29_000295"
     CDS             76543..>76916
                     /locus_tag="KPH29_000295"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG1465940.1"
                     /translation="MSRKYSISSMCKLIGVSRSGYYKWLSYSKKSSDRGIKDRIIKDY
                     IIEIHKKYRGTYGRKRICTYLNKILDSPINHKKVYRLMKELGIKSIIRKKVYRRKFKS
                     YEVYDNILNREFRANQPLEKIC"
BASE COUNT        30597 a         8051 c        13986 g        24282 t
ORIGIN      
        1 ttttttgttt agataacaat ttatcttgtt taaaaatttt ctatttgaaa ataatctaaa
       61 taatatactt ttatatggga gtgattataa ttcaaataag aagaccatta ttaattatat
      121 taatgtttgt ggtagctata tcatttatca ttacaaataa cacaaaaaac gacgaagaac
      181 ttaatgataa gataataact atacaagggg ttgtcaaagg aaaaattgaa aagaaaagat
      241 acaatcagta caaggtaggg gtttttctaa taaatgatta tacaaaaaat aaaaatttga
      301 agattggtca gaaagttaat attacaggta aattcaaaag tttagataaa atgaagtatg
      361 atgattttga ttatggaagg tatataaaaa gtactggata taaaggtata gtgtatataa
      421 ataactataa aattattgat aaaaataaac tttactcttt aataggggaa ataaagtttt
      481 atattagtaa aacttatagg tacttatata agaaaaactc agattttata aattctatac
      541 tattagcaga agttgaaaat ttgactgagg aacaaaaaga aatttttact agaactggaa
      601 ctagtcatgt aatatctata tcaggtcttc atacaggaat attatgtgta attatatcct
      661 ttctattaag aggtataaat aaattgtata aattattaat actgtcgatt tttataactt
      721 tatattgtat aatggtaggt gcgtctccat ccataattag gtctatagca tttgttatga
      781 ttttttattt atcaatattt attgatagaa agaaagatgg aatatcagca ttgtctctaa
      841 ttgggataat tttaattatg aataacccct atgttatata taatgtaagt tttcaattgt
      901 cttttttagc tacactttct atattaatat tctataataa aataaatagt attataaaat
      961 taagtatggt atcattgaca atatcatcaa atatattaac tttacctata atttattata
     1021 catttaaagg tataccattg ctttcaatta taggaaattt aattatagta ccatttgtgg
     1081 gagtaataat gtatttaagt attgcaagtt taatcgtatt taaagtaagt gtagtaatag
     1141 ctaaaattat atcttttttt aatagtacat tgatagaaag catatttttt ttactagaaa
     1201 aaatcagcaa tttaagtttt gcatacatta atatagaaag tcctaaattt tatattgttg
     1261 ttatttatta cataggagtg tttttttaca tattttacat agaaggaaaa gagataaagg
     1321 agcaagagaa tgaattacaa ggatattata aggaatgtaa aagagaaaaa ctttgaaaaa
     1381 atgtatcttt tttatggaag agagtattat ttaattgaga atgcaataaa agcatttaaa
     1441 gatagtctaa acgaaggaat gcttgatttt aatttagata taatagatgg gaaggaaata
     1501 gtcttaaatc agcttataag ctctatagaa acacttccat ttatggatga tagaaagata
     1561 gtaataataa aagacttcga actattaaaa ggaaagaaaa agaattttac agatagtgat
     1621 gagaaatact taatagagca tttagataat attccagaca ctacaactat agtttttgtt
     1681 gtatatggag atgtagacaa aagaaaatca ttagttaaga aaataggaaa caatggaatt
     1741 gtttttgatt gtgataagct ttctgatatg gatttattta aatggataaa aaaatctttt
     1801 gctttaaatg atgttataat agataattct caaatcatgt attttattga acaagaagga
     1861 tacagagata agagcagtga aaagacatta tctgatttag aaaatgaaat aaataaaata
     1921 agttcctttg ttggtaaagg gaataatgta accaatgacg taattgataa attatctcaa
     1981 aaaaaagttg agaatgatat atttaaatta atcgattata ttggagaaca aaatgcttct
     2041 aatgcaatga aaatattaaa tgatatgata caagaaggcg aatctgtatt aggtatattt
     2101 tctatgatag caagacaatt taaaataatt atgcaagtaa gacagttaca acttgatgga
     2161 tattcaacaa aacttatagc tgataaatta aaaatgcatc aatttgttgt aggaaaagca
     2221 ttaaagcaaa ctaaaaattt ttctgatgat ataattgtgg aaatattaaa ctatatatta
     2281 gaaagtgatt ataaaataaa aacaggtctt ataagagata ctttagcagt tgaaatgttg
     2341 gttagtagat actgtaaaag agaagcaatt taaaaaatct atatattaat aaaaaataaa
     2401 tagcaagata tgttatagtt cagagtagat gtaagctaaa ttaagtttga tatacaatca
     2461 actataatat gtctaactat ttatttttta ttgtataaat ttacaaaata aaaaacccct
     2521 acataggggt tattatatat aagaaatata aactggtaat taagcgttca taccgtttaa
     2581 tttttgagct aatttagcta cttttctagc tgcagcattc ttatgtatag ttcctttaga
     2641 agctacttgg tatattcttt tttgagcgta ttggaacttt gcaacagcat cctctctgtt
     2701 tccagctgca acagcatctt cgaatcttct tattgcagtt tttatttgag attttctaac
     2761 tctatttaaa gcagttttct tttcgataac acttattctt ttcttagctg attttatatt
     2821 tgccaccgtg ttcacctccc tgtatttttt tatcatattc atcgtcaaca tattagattt
     2881 taacagaaat aaatctaaaa atcaaggatt attatttata tattttaata atgtttttta
     2941 ttttgatttt ttagactgta aattgtgtat aaataatttg caatatcttg tttttaaatt
     3001 ggtatatttt gtgttttaaa tattttgaca tatataaatt aatattttat ttttgtaaca
     3061 aatattttga gataatacaa actattaaca tacattttga tagtatttcc aataattata
     3121 agaaaataca agaaattact catttttgta aatttaatga aaaattataa aaatataaat
     3181 ttatatgctt aattaagata atttttccaa taatcaagat aatttttttc attagtatgt
     3241 atgatagatg catcaaacaa tataactaat atgttagaag gttatatgta attaatctca
     3301 gtgtttatat agaatagcat ttgtagcatt tattatagtt gtaattacta cttatatcat
     3361 agacttgaat agtctaaaaa aatagactat aatattttta tagactaaag aaagggctga
     3421 taagatggac atagcaaaac taattttaaa tcctgcaagg cttcgtatct tacaatatat
     3481 tcgacttcat ggcagtgctc gcacatctga tattgtaaag tatttaaatg atattcctcg
     3541 tgcaacagtt tatcaccatg taaaaatttt agaagagaac aatatgattg aagtaattaa
     3601 agaaaatcgt gtgcgtggta ctattgaaaa ggtttatgca ctaaaggaat atacaacatc
     3661 tatggaagga gagaccttcg ttgcactttc tacagcattt tatgtaggtc tgatgcagga
     3721 aatgaatgaa tactttagca gaaaagagca agaccataaa aaggataatg tatttttctc
     3781 ttcagcatta ctatatatga gtgataatga atatgaaaat cttcttaaaa gtatagctga
     3841 tttattgaaa ccatatatag aacaaaagcc taaatcaaat ctaaaactta gaaaactttc
     3901 aattatatct tctccacctg tggttgataa tgataaaaca aaataacatg aatcttattt
     3961 ttataaaagg aggttgtata tgaaatttca tgaatttgga gataaaaaca aaccacatat
     4021 tatgttgatt catgggggag ggaatgcgtg gtggaactat ttgcgacaag cagaagtttt
     4081 gtctaacaac tatcatgtga tacttcctac attggatggt catggagaag aatatgaaac
     4141 aacatacatt tctacagagg atacagcaga taagttgata tcatatatag atgaaaattg
     4201 cagtggacat cttttcgctt tgtgtggggt atcacttggt ggtcaaattg tcatggaact
     4261 tttatctcga aaacctaata ttactaaaaa agcaattatt gatggtagta tttgctaccc
     4321 aagacctctt atggcaaggt tttgtattgc tggtattcga ttttttggag gacttttgtt
     4381 tagtgagaag gcttgtcgct ttcaaattgc tatgatgcca aaattgttac ctgaaaatat
     4441 gcaatatcca gatgaaataa aagcgtatta tatgacagat atgccacatg taagaaaaga
     4501 aacattctat aatatgtatc gtacttatat gatgaactat acacttaaag agaatgttaa
     4561 aaaaaccaca gcacaagtaa tgtactggta tggagaaaaa gaaatgaagt gcgttaaaaa
     4621 atctgcaaaa atgtttcagt cttatgtacc ctcttgtgag atttatgaag ctaagggcta
     4681 taatcatggg tatttatcgc tttatcttcc aaatgaatgg ttggaaattg cagaaccatt
     4741 tttccaaaag gaataaaggt gttaggtgat tattgtataa ttattgctgt ttttttatat
     4801 agtaaataaa ttaggtataa atataaaaaa gttattttag ttaatatatt agtgaaagct
     4861 taataggaaa attataaata gattaaaggt agcgcctata taaatagttg ctaccttttt
     4921 ttgcttgtaa ataaatgata gataagaaag tttactgaca aatcactgac taaacccaat
     4981 tttcttacat tgaaagcctt aattttactt gttttaaata aatttacaat ttcttgttat
     5041 aaaatactaa attttgttaa caattataat tagcatttaa tttatactgg aggtttatat
     5101 gattaatgta agaacagatt tagctttaga agctagtgaa atgtgtgaaa agtctcaaga
     5161 aggaagtagt attcctggtg taaaaataga aactaaagaa ttagaaaatt gcattgtaac
     5221 taaagttgaa gtaatagaca ggcaaggcgc agagataatg aataaagata taggaaagta
     5281 tataactctg gaaagtaatc ttatgaagtt tgatgatgat gagtcgagag aagaaatgat
     5341 aagttattta aaagaggagt tagtagatat atttggccaa gacaaaaata aaaaaaccct
     5401 ggtaattggt cttggaaata gaaatattac ttctgatgcg ttaggaccaa agagtgtatc
     5461 aaaaacactt gtaactagac atctttttaa aaattataat aaagactatg atgatgattt
     5521 cactgaggta tcagctttaa gcccaggagt tatgggtgtt actggaatag aaactagtga
     5581 aatagtaaag tcattggttg aaaaagtaaa accagataga gtagtagcaa tagacgcatt
     5641 ggcttcaaga aaaatggaaa gagtaaactc tactatacag atatcaacag caggaatatc
     5701 acctggtgga ggtgttggca atactagaaa atctctaact aaagaaactt taggcgtgga
     5761 tgttattgca ataggcgttc caacggtggt tgatgcagca actcttacaa tagatgtctt
     5821 agatatggca atagacaatt taatagctca atcagaagaa acagaaagtt tttatgaaat
     5881 gttgaaaaaa cttaaagaag aagaaaaata tcatctaata aaagattcgt tagacccata
     5941 tgacaagaac ttaatagtaa caccaaagga tatagatgat acaatcgaaa atctatctat
     6001 aataataagt gaaggcttaa ataggtcttt acatccaggt agattggtat aaaataagga
     6061 ggagcaatta tgtttaaaaa atgcattaaa gtagtaacct tgacttttat attagcatgc
     6121 attttaccag gaaaatcatt agctttgaat caggatgatt ttttaaagtt tttagtaaat
     6181 tcatcttatc cagaagctaa agtcgaagga aatgatacag aaaataaaaa gaataataaa
     6241 aataaagaaa caagcaaaga aaataaggaa gaaagtaaag aagaaaacac taaatctaaa
     6301 gatgcaagta aagttgataa taaaaaagag agtgaaaaag aatatataaa actttatgtt
     6361 ggtaaagaaa atgttccaga tattgagtca aaaaattcag acacaactga aactaacaca
     6421 acatctagtt cagattataa ggatgattta agagtaacca aagaaaatcc aagaattttg
     6481 atatatcata ctcatggatg tgagacttat tcaaattcac ctgatggaaa ttatcattca
     6541 agagataaga aaaattcagt aatggaagta ggaagtgcat taactagtgc tttagatagc
     6601 aaaggatggg gtgtagtcca tactactaaa taccatgact atccatctta taataactct
     6661 tatgcaagta gcttaaaaac aatacaaagt atattaccta aatataactc agtggatata
     6721 gcgatagact tacatagaga tgctagggat ttaactaatc cagccactaa ggaaaaagac
     6781 catttaaaat atacaactat gataaatgga gagagagtat caaagttctt ctttgttgta
     6841 ggaggtaaaa atacaaatag aaaacaactt agagctttag cagaagatat aactgctttt
     6901 gctgagaaaa agtatcctgg tctagtatct ccaatagtag aaaaagacta tgctagattc
     6961 aatcagtttg cagttaagaa tcatatgtta gtagaaatag ggaataacgc aacaagtgta
     7021 gaagaatcta aggctactac aaagtattta gccgagattt tagatgaata ttttaaacaa
     7081 aaaaattaga gctttaagtt ataattagga gcatgctaaa tgagccgatt agaaaaaaat
     7141 atagaaagta aaagtaaact aagaaaatac aagtatatta tgagaatctt atttttgatt
     7201 actatgatta taggtacatc agtttgtgta tttatagtag acagtaatgc aaaaagtatg
     7261 ctaggaatga aacaatatga ttttccaagt attaaatttg atataacaaa atttgatttt
     7321 gacataccaa attttgatat acatgaaatc ataaaaaaat ttaataagta gtaataagtg
     7381 aataaggttt attgattaaa ataagcctta ttttagttat atatttttaa tgtataaaat
     7441 aaaaattcat taactgtatt ttataggatt tactttaagt ttacgatatt atataatatt
     7501 tagtgttaga cttgataatc cacatataat gtggataatt ttgtttagtg ccaattagtt
     7561 tatattcaaa aattttaaat ttggtatata aagagttttt agataatgta aaataataac
     7621 tataagaata atcaagagga gggaatttta aggtggacaa taaacaaagt agaactagaa
     7681 attttagtat aattgcacat atagaccatg gaaagtctac cttagctgat agattaatac
     7741 aacaaactgg gcttgttagt gaaagagaca tgaaaagtca attacttgac aatatggacc
     7801 ttgaaagaga aagaggaatc actataaagc ttcaaaacat aagattaatg tataaagcga
     7861 aggatggaaa tgaatattat ttaaatctta tagatacacc tggacatgta gactttaact
     7921 atgaggtatc aagaagttta gctgcatgtg aaggagcact attagtagtt gatgcagctc
     7981 aaggtgttga ggcacaaact ttagccaatg tttatcttgc tatagaccaa gatttagaaa
     8041 tattaccaat aataaataaa atagatttac caagtgcaag accagaggaa gtaaaaaatg
     8101 aaatagaaga tttaataggg cttgattcaa gtgaagctcc tcttatttca gctaaaactg
     8161 gtttaaacat agaagatgtt ttagaagata ttgtaaaaaa tgttcctcca cctaagggag
     8221 ataatgaagc tccattaaag gcattaatat tcgattctta ctatgatgct tacaaaggtg
     8281 ttgtagctta tgttagagta tttgaaggaa ctgttaaaaa gggtatgaca ataaaaatga
     8341 tgaataccaa taaaaagttt gaggtaactg aagttggtgt tatggctcca ggtcaaactg
     8401 aacttagtga attatcagca ggtgatgttg gttatattgc tgctagtata aaagatataa
     8461 gaagttgtcg tgttggtgat acaataacag attcaaataa tcctacagaa gaacctttac
     8521 caggatacaa aaaagctact ccaatggtat attgtggtat atatccagga gaaggagaaa
     8581 agtatgagaa tgttagagat gctcttgaaa agcttcaagt aaatgatgct gcacttgaat
     8641 atgaagctga gacttcagca gcactaggat ttggttttag atgtggtttc ttaggtcttt
     8701 tacatatgga gataatgcaa gaaagacttg agagagaatt taaccttgat ataattacta
     8761 cagctccatc tgttatatat agagttacaa aaatggatgg agaggttgta atgatacaaa
     8821 atcctgcaaa cttaccagaa ccatctgaga taaagatgat agaagagcct atagtaaaag
     8881 gagatataat agttcctaaa gactatgtgg gtgttgttat ggagctttgc caggaaagac
     8941 gtggaaatat gttaaatatg gaatatatag atgagagaag agtaatgctt cactatgacc
     9001 ttcctctcaa tgaagttgtt tacgatttct ttgatgcttt aaaatcgaga actagaggtt
     9061 atggttctct tgattatgag gttaaaggtt atgtagcatc tacacttgta aaacttgata
     9121 tattaatcaa taaagaacaa gtggatgcac ttagttttat tgtccatgag acacgtgctt
     9181 tccctagagg aaaggcaatg tgtgagaaat taaaaggaga aattccaaga catcagtttg
     9241 ctatacctat acaagctgct gttggtaata aggttattgc aagagaaaca ataagcgctc
     9301 taagaaaaga tgttcttgct aaatgttatg gtggggatat atctcgtaag aagaaacttc
     9361 ttgaaaagca aaaagaaggt aagaagcgta tgagacaaat tggttctgtt gaagttcctc
     9421 aaaaagcatt tatgtctgta cttaaactag atgaataaat tcttataaat ttagataaat
     9481 atatttaaaa gctagagtga ttttagaatt atttctaaat taatctagct tttttgtggt
     9541 atattttaag taaactcata gagaatataa gtaagaatat attaaagaag aataaaaagg
     9601 tgatatttat gattaatata gaaattccta aaaaagtaga ctatataatt aaagaattag
     9661 aaacgaatgg ttatgaagct tatgtagttg gaggatgcgt tagagattgt ttattagaaa
     9721 gagttccaaa cgattgggat ataacaacta gtgctagacc agaagtggta gtagaattat
     9781 ttgaaaaaac aattccaact ggtatacaac atgggactgt aacagttatg attgaacatg
     9841 aaccatttga agtgacaact tatagaattg atggaaatta tagtgatggt agacatccag
     9901 attcaataga atttacaaat aacatagtta aggacttgtc aagaagagat tttactatca
     9961 attcaattgc atacaattca aagacaggat tagtcgaccc atttaatggc tatgaagata
    10021 tacaaaataa gtatatacgt tgtgttggaa atccagtaga tagatttgaa gaagatgcac
    10081 ttagaatgct tagggcagtt agattttcag cacaacttaa ttttaaaata gctgaaggta
    10141 ctaagcaaag tattcataaa aaagcagatt tgataaaaaa tgtttctatc gaaagaatcc
    10201 agacagagtt taataaaata ttagtatctg attcatctaa attaaactta ttaaaatcaa
    10261 caggtttgtt gaaatttata attcctgaaa tatgtgaatt agaagatgtt actcaacaca
    10321 atccatatca catctatgat gtacaaaaac atactttaat atctacagaa gtaattgaag
    10381 atgaactgta tttaaaactt acaatgttgt ttcatgattt aggtaaaaaa gtaactaaaa
    10441 caactgataa aaatggtgtt gaccattttt atactcatag tagagaatct gtaaagattg
    10501 caaagaagat tttgagaaga cttaagtatg ataattatac tattaataaa gttttaatat
    10561 taatacaata tcatgattat agaatagaac ctaaaaggaa gataattaaa aagttattaa
    10621 ataaacttga agatgttgaa ttatttgaag atttaataaa agttaattgg gctgatacac
    10681 ttgccaaaaa cccaaagtat gcaaaacaaa agatattaaa tcttattgag tgtgaaaaag
    10741 aatttaaaca tataataaat caaaaggagt gctttaatct taaagattta gctattaatg
    10801 gaaaagattt gatatctata ggtataaaac caggtaaaga tataggacat attttaaata
    10861 agatgcttga aatagtcata aataatccag aattaaatga aaaagaaata ctaaaagaaa
    10921 aagcattaaa tatatataca ttttagattc aaatataaaa aatttaatta ctttaaaaaa
    10981 ttatagaaga aaatacttat ttagataaaa taaataattt ttagagaata ttttagaagt
    11041 tacgattgta tgatttttaa acgcatagca tttactctag gactatagac taatataaaa
    11101 aaaaactttg ggaatactaa aaccataaat ttataaaatt aatattttat atactgtaaa
    11161 acagtgagga gggacagtat gcatactcaa ctacaaaaga ctattggtct ttcagcggct
    11221 ctttctactg ttgtaggtat ggttataggt tcgggagttt tttttaagcc acaagcaata
    11281 tacactacaa ctaatggagc tccaggtcta ggtattatag cgtggctttt aggtggtttt
    11341 ataacaataa ctgctgggct tacagctaca gaaatttcag ctgctatacc aaaaactggt
    11401 ggtatgatga tttatatcga agaaatatat ggagaaaagt taggcttttt aactggatgg
    11461 atgcaaactg tattattttt cccaggtaca tcagcagcat taggagtaat atttgctcaa
    11521 caagcatctg aacttttagg aatgagccca aataatatgg caaatgtact tcctatagca
    11581 attggagtta tactattttt agcgttatta aatataatag gttcatcctt aggaggaaaa
    11641 gttcagacag tagccactat tggaaagatg atacctttaa ttttaataat agtctttgga
    11701 tttattaaag gtcaaagtag tgaagtatta aatccatttg taggagatgg tgtaaatgca
    11761 tcaaatgcac ttggacaagt attaatagct actttgtttg catatgatgg ttggataaat
    11821 gttggagcta tatcaggaga aatgaagtct ccagagaaag atttaccaag agcaatagtt
    11881 ggtggtttat cattagttat ggcagtttat attataataa atatagctta tctttgggta
    11941 gtaccagcaa gtgaattagc aacagttact tccccagcta cattagtagc aacaagattg
    12001 tttggaaata taggtggaaa agtaataact gtgggtatat taatatcagt atttgggaca
    12061 ttgaatggat atttattaac aggttcaagg ataccttata cattagctga aatgggaact
    12121 ttaccagcat ctaaaactct tttaaaagta aattctggag gttctcctgt aaattcaata
    12181 ctacttataa ctgtgttagc ctgtgtatat gcattatctg gtcaatttaa tcttttgaca
    12241 gacttaacta tattttcaat atgggtattt tatgtattga cttttatagg tgttatgagg
    12301 cttagaagac aaaagccaga tttacacaga ccatataaag tccctttata tccaataata
    12361 ccaattatag ctatactagg tggactgttt gtaataataa atcaaatatt gacttcaaca
    12421 attatatctt taggtggaat atttataaca ctattaggtt taccagttta ttattatatg
    12481 aaaaaaagat aactacttaa tttaagttag ataaatttgg gtaaaggtga aaattaactt
    12541 ttatccaaat tttttatact gttatacaat ataagcatta tatattactt gatgtgttat
    12601 tttgtttgat ttataattta gtaattgatg ctttaagata atttaagaaa tgaggaataa
    12661 aaaatgctag gactttatgt acatattcca ttttgtgtga aaaaatgtaa gtattgtgat
    12721 tttaattctt ataaaatgga catagattca aaaaaaagat atatagaaga cttaaaaata
    12781 gaaatgaaat tatatagtaa taaattatat aaagataata aatataaaaa taaagaatgc
    12841 tgtagtctaa ataaaaatga caaaataacg agtatatttg tagggggagg tacacctagt
    12901 atactaacat ctgatgaaat aagagaagta tttataagta taaaagaaat gtttgatata
    12961 gatgagaatg cagaaataac tatagagtgt aatcctggga cattaacatt agaaaaatta
    13021 aaaactatga aagagattgg cataaataga cttagtatag ggctacaggc cattcaagaa
    13081 aagcatttga attttatagg aagaatacat acttatgaag aatttgagaa aaactataaa
    13141 gatgcattaa gtgtaggttt taaaaatatt aatgtagatt taatgtacag tcttccaaat
    13201 caaactctat gtgactggaa ggaaactctt gaaaaagttg tacacttaaa tccaacacat
    13261 atatcagcgt attcccttat actagaagaa ggaactgaac tatataatat gtatgaaagt
    13321 aataaatttg agcttataga tgaaaatgta gatatagaaa tgtatgagta tacaataaac
    13381 tacttaaagt caaaaggata taatcaatat gaaatatcaa attactcaaa agaaggctat
    13441 aattgtgagc ataatatttt atattgggaa tgtgaacatt atatagggat tggagcagga
    13501 gcttctggct atattaatga gaacagatac aataatgtag aatctttaga agattatcat
    13561 ttaagtttgg taaaaagaga aaaaccaata caagaaaatg aaattctttc tgagaaagat
    13621 atgatagaag aaaagatatt tatggggctt agaatgaata aaggcataaa atttgaagat
    13681 tttaaaaaaa aatttggaat agattttaga gagaagtata ataagcaaat agaaatgcta
    13741 ttagctagaa agcttataaa tcagagcttt gaaggtatac aattaactca aaaaggaaga
    13801 gaaatatcta atagtgtatt tatagaattt atggaataat ttttaaggtg ttgacaaaat
    13861 aaatttataa tggtatataa tatatataat ctttttagca ctcgaactta gtgagtgcta
    13921 acagctagga ggtataataa tatggaatta aatgaaagaa aattaaatat cctcaaagct
    13981 atagttaaag attatataga aacagctgaa gctataggtt ctagaacaat atctaaaaga
    14041 catgatttag gtgttagtgc tgctaccata agaaatgaaa tggctgacct tgaagaatta
    14101 gggtatttaa ttcagcctca tacttctgcg ggtagagttc catctgaaaa aggctataag
    14161 ttatatgtaa actctcttat gagtaaaagc gaattggatg ataatgataa gattcttatt
    14221 gaacaatgta tgaatcataa tataaatcat ataaaagaat tgattcatga aacctctaag
    14281 ctattatcgc aactaacaaa ctatacaact gtagcagtta ctaagagttt aataaatcaa
    14341 agtgttataa agcacataca gttggttgct atgaatgaca ataatatttt acttatagtt
    14401 gtaacagata aaggggactt aaaaaaagct aaccttacaa ctaatgtata tttagatcaa
    14461 tctaagttaa atttgatttc tgataatctt acaaggaagc ttttaggtaa aagtataaca
    14521 gatttggatg ataatctaat tgcttttatt aaatacgaaa taagtgagta ttctggactt
    14581 atagatgagc ttttaaatgc tttgaactca aatatgaaag aagaagattt ttctctatca
    14641 ttaaatggag ctactaacat atttagttat ccagaattta atgatgtatt aaaagcaaag
    14701 tcatttttaa atatgttgga aaaaaaggaa acaatagctg acataattaa atcaaaggga
    14761 atacaaaaag acaatctaaa tataataatt ggtagtgata atgattgtga actggcacag
    14821 gattgtagta tagttacagc aacttataat gtagatagag acttggttgg gcgaataagc
    14881 tttataggcc ctaccagaat ggattatgct agaatttact ctataataaa ttatatgagt
    14941 ttattaatta atagaaagta aagaaatttt aaattgcgag gtgtaaagtc ttggataaaa
    15001 aaaatgagca acaggaagta agagaagaaa atgatacttc tattaaccaa gaatcggaaa
    15061 ctcaagtaga gttagaagag gaagttgtta atgaagaatg tgaaacatca agtgaaaaaa
    15121 ctgatgaaaa agaagttgat gatgaaaatg taacagatat aaattctaag ttagcagaaa
    15181 aaaaattgca ggatgagttg gatgaattaa atgataaata tcaaagactt caagccgaat
    15241 atgcaaacta cagaagaaga actcaacaag agaaagagac aataggcgta tttgcaaatg
    15301 aaaaaataat aactgagttg ataccagtta tagatagtat ggaaagagcc ttagatgcat
    15361 gtgaagataa ggaagatact atgtataagg gtataagcct tgtacataaa caattaatag
    15421 acactttggt aaaatttgga gtagaagaaa tagaagcaga atctaaggaa tttgacccta
    15481 atctacatct agcagttatg caagaaagtg tagatggagt tgaagcaaat caaatagtaa
    15541 tggtacttca aaaaggatat aagctaggaa ctaaagttgt aagaccttca atggttaaag
    15601 tttcttgcta agtataaatt aaaggttaaa ttatttaacc taatataaaa tattaaaata
    15661 agtaaaactt aattatataa ttactcgagg aggattttag tcatgggaaa aataatagga
    15721 atagatttag gaacaacaaa ttcatgtgtt gcagtgttag aaggtggaga agcacaaata
    15781 atagcaaata gtgaaggtat gagaactact ccatcagtag tagcatttac aaaagatggt
    15841 gagagaatag ttggagaacc tgcaaaaaga caagcagtta caaatgcaga taaaacaata
    15901 acttctataa aaactcatat gggaactgat tataaagtaa atatagatgg aaaatcatat
    15961 actccacaag aaatatcagc aataatatta caaaaattaa aatcagatgc agaaagttac
    16021 ttaggacaaa cagtaacaga agcagttata acagtaccag cttactttac agatgctcaa
    16081 agacaagcga ctaaagatgc tggtagaata gcagggcttg atgttaagag aataataaat
    16141 gagccaacag cagcagctct tgcttatggt atggataaat tagaccaaga aaagaaaata
    16201 ttagtatttg acttaggtgg aggaactttt gacgtatcta tacttgaaat aggagatgga
    16261 acttttgaag ttttagctac agctggtaac aatagattag gtggagatga ctttgaccaa
    16321 atagtaatag attatttagc agatgaattt aaaaaagctg aaggtgtaga tttaagaaat
    16381 gataaaatgg ctcttcaaag attaaaagaa gcagcagaaa aggctaagaa agaattatca
    16441 tcaacaatga gttcaaacat aaacttacct ttcataactg ctacagcaga aggaccaaaa
    16501 cacttaaata tagatttatc aagagctaaa tttgaagaat taactagagg tttagttgaa
    16561 aaaactatgg aaccaactaa gacagcttta caagatgcag gactttctac aggtgatata
    16621 gatgatgtat tattagtagg tggttctaca agaataccag cagtacaaga agctgttaag
    16681 aagtttatag gaaaagaacc tcataaaggt ataaacccag atgagtgtgt tgcagcaggt
    16741 gcttctatac aagctggagt tttagcaggg gatgttaaag atttattatt actagatgtt
    16801 actccattat ctcttggtat agaaactatg ggtaatgtaa tgactaagat aatagaaaga
    16861 aatactacaa taccaactaa gaagtctcaa atattctcaa ctgctgctga taaccaaaca
    16921 gctgttgata tacatgttct tcaaggtgaa agaagtatgg cttatgacaa tacaacttta
    16981 ggaagattcc aattaacaga tataccacca gcacaaagag gaatacctca aatagaagtt
    17041 acttttgata tagatgcaaa tggtatagtt aatgtttcag ctaaagattt aggtacagga
    17101 aaagagcaaa aaataactat aacatcaaac actaatttat cagaagcaga aatagagcaa
    17161 aagataaaag aagcagaaat gaatgcagaa gctgacaaac aaaagaaaga aaaaatagaa
    17221 gcattcaatc aagctgaatc tactatatat caaactgaaa aaactttaaa tgaacttgga
    17281 gataaaataa gttcaggtga aaaagaggat atagaaaaag ctatagcaga cttaaaagct
    17341 gtaaaagata atcaagatgc tacagcagaa gaacttaaga aagctacaga tgaagtaatg
    17401 actaagttcc aaaaagtatc tcaagaaatg taccaaaaag cagctcaaga acaacaagcg
    17461 gcacaaggtg cagaacaagc acaagacaat ggacctaaag atgacaatgt agtagatgct
    17521 gactttaaag aagtagatga agataaataa gctatataat atgtaacact aaatgataat
    17581 tggaacatat atgatttgtg aaaatcatat agatgttaaa gatatatgga caatacacat
    17641 cataaagaat ataatatatc atagattata taacttaaag tagaatataa gaattaatga
    17701 tagcttgtag tttattattc tacaagctat cgttttgtaa aatgaaggtg gtgtcaattt
    17761 ttgagtacaa aaagggatta ttatgaagtc ttaggtatca gtaaagatgc agaagctcaa
    17821 gagataaaaa aagcttatag aaaattagca atgaagtacc atccagatag aaatcctgga
    17881 gataaagagg cggaagaaaa atttaaagag ataaatgaag cttatgaagt tctatctgat
    17941 gatacaaaga gaaaaactta tgaccagttt ggacatgatg gattaaatgg tcaaggtggt
    18001 tttggtggtc aaggaggatt tggcggtcaa ggctttggtg gttttgaaga tatgttcgga
    18061 gatatatttg gtgacatgtt tggaggaagc ttcggaggtg gaagagcaag aagaagaggg
    18121 cctcaacgtg gtgcagatat aagacagagt gttactatat cttttgaaga agctgcattt
    18181 ggtaaaaaga tgtctataaa agtaaataga agtgaagagt gtgaagaatg taatggaact
    18241 ggagcaaaac caggaacatc aaagaaaact tgttcaactt gtaatggaac tggccaagtt
    18301 agaactgttc aaagaactcc atttggtaat atagcaagtt caagaccatg tagtgcctgt
    18361 aatggaactg gagaagtgat tgaatctcca tgtagtaaat gtcatggaac tggaaataca
    18421 agaaaagtta agactataga agtagatata cctgctggta tagatgatgg tcaaatgata
    18481 aaactttctg gtcaaggtga agtaggagaa aaaggtgctc caagaggaga cttatatatt
    18541 gtagttaatg ttaagtcaca tccactattt actagagatg gaaatgatat ttattttgaa
    18601 atgccaatta catttgttca agcaacttta ggtgatgaga tagaagtacc tactttggat
    18661 ggaaaagtta aatatagtgt gccagagggg acacaaactg gaactgtatt tagacttaaa
    18721 gagaaaggaa tccctagaat tagaggtaat tcaagaggag accaatatgt taaggttgta
    18781 gttgaaattc ctaaaaaatt aaacgataag caaaaagaac tattgagaga atttgcgaag
    18841 gagtgtgggt cgaacgtcca cgaaaagaag aaaaccttcg gacaaaagat agaagatatg
    18901 tttaagaaaa agtagttaaa gtagccaata tataatcata tttttgaaat cttagttatt
    18961 tataagattg tcaaaggaga tattatgatt tttatattgg ctatttttgt ataaaaaata
    19021 aatgtgcata aaataaaact gaatataaat aattttctga aaaatacttg tcctaaaata
    19081 tataatgtgc tataatataa tcaaataaaa gaaaggggtg attccagtgg gaacggatgt
    19141 ggcactgcca aatgatgaag aaaactttat tgtaatatac aaaataaaat tagatacttt
    19201 tagtgctaga ttaagtactg gaaaaactca tgagctggga taattattgt aattacctag
    19261 actcaaactt ttagtagtaa aatagtaata ttatatagac aaggtatcta atttttaaat
    19321 cttttgtatg agttaaaatt tttataaagg gtatatctga ttagaatgta attagggaaa
    19381 cctagttgtg aattactttt agtatataca tgtgtaaaga aatatgtata tggtattggt
    19441 tattgtgcta gatgtgtatg taaaggaata tgtgtctagt tgggtatatg taaagaaata
    19501 tacttataat ctatgaaaaa tttttagtat aaattgctaa aacattgaga ttgtttttta
    19561 tagaaagtta gtttatacca actaaaaatg tgcttaagat ttgtttgatg tttaattata
    19621 aataaggttg tagataaaaa aataagtaaa gacaaaggtt taaaatactt gctttaaata
    19681 tagtttgttt tgaacacttg ccaaattaaa ataattaata aaatagacat cttctcatag
    19741 taaaatatac tgtgagagga tgtttttgct ttgttcataa acttttatga aaaatttgca
    19801 tagtgtcaca ttttaatata ctatatatat aataaaatat agataaaatt aaatgattca
    19861 aaagagaaag gtgaaaagag atgtattata taggtgtaga cataggagga acaggtatac
    19921 aagcaggagt agttgataac tatgggaaga taatttttag gtcagagtgt aaaacagtaa
    19981 ttgaaaaagg ctttgaagga atacttaatg acataaaaat aatgatttat aagttgttgg
    20041 aagataataa acttactatg agtgacataa aatctattgg atttggtgtt ccaggtttta
    20101 taaataaaga aggattagta acttgtgtaa atttaaaatg gaataaaaaa gcttttaata
    20161 aagagttaaa aagaagattt ccagatgctg aaattcatgg agaaaatgat gcaacagtag
    20221 cagcacttgg agaagcaaag tttggaagta tgaaaggtgc taatgttgga gtattatata
    20281 ctttaggaac tggtgtaggt ggaggaatag taattaatca aaaagtcttt tctggagcac
    20341 atggattggg ttcagaaata ggtcatcaaa ttataggaga aaactatttt aattgtaatt
    20401 gtggaaataa tggttgtgta gaaacttttt gttcagcaac tgcaattata aagtattctc
    20461 aaaaactgat agaagaagga gaaaagagta ggattttaga tttggcagag ggaaatttag
    20521 aaaatgtaaa tgcaaagatg gtgtttgatg catatagaga aaatgattta gtggcaatta
    20581 aagtaataaa tagatttaaa gattatttag caaaaacttt tgcaaacaca ataaattctt
    20641 tagacccaga aattatatct ataggaggag gcatttctaa atctagcgat ataatacttg
    20701 atggaataga agatttggta agaaaatttg tattatacaa aacagaagat atagctacaa
    20761 ttacatgtgc aactttaggt tcagacgcag gaattatagg agcagctttt ttataatgta
    20821 aatgaactgc ttattggagg cgtgatgata tatgggtaaa acaaatgcta gaagaacgct
    20881 ttttttgata gcaataggtt caggaactat gctgaatcct cttaattctt ctatgatttc
    20941 acttgcttta catagtatac aaaatgattt tcatattagt tttacaactg tatcttggat
    21001 tgtttcagca ttttacctag ctagtgctat tgcacaacca gtttcaggta agataggaga
    21061 tttaataggt agaaaggtct tatttttgag tggtttgatg cttgtaattt tatcagcatc
    21121 catagtacca cttgttcaat cattctttat attgattttt ataagagtaa ttcaagcaat
    21181 tggaacaagt accctatatc catctggagt tgctcttata caaaataata taaaagaaag
    21241 acaatcttct gcattagcag ttctaaccat ttttgcttca actacagcag gattaggtcc
    21301 gacacttgga ggattattgt tggacttagg aggatggcat gctatatttc ttgtaaatat
    21361 acctgttgtt ttggtttcta tatgcttagg gtatttttta ttcccaaaag aagtaaaaga
    21421 aaagaaatct ataaaagaaa ctttaaaaaa tgttttgtca agattagata ttattggcat
    21481 acttctattt tcaattgcaa tgatttttat attactattt ttgctatcta tgaaggaatc
    21541 ttttaactta gaacaacttg tatttggtat aatactgatg tgtcttttta tttggcatga
    21601 attgagaaca aaattgccat ttatagatat taagcttttt gtaagtaatc ctaaattgtc
    21661 aaaagtttac ttgcaattta ttatattaaa tttctttaat tatattttgt tttttggtct
    21721 tcctagttat tttcaagatg ccttacatta tagtgcaaag tcgactggat tatttatgct
    21781 atttatgtct ggtataggta tatttatatc tccattaact ggaaaatgga tagataaatc
    21841 tggaactaga tttcctcttg ttacatcatc aatctttatg tttattagtg cagtacttct
    21901 ttcagtagtt tttatccata tacctgtaat aggaaagggt attatattat cattagcagg
    21961 tattagttat ggagttggta atgtagccct gcaatcctct atgtttgaag aaagtcctcg
    22021 tgacagtata gggacagctt ctggtctgtt tcaaactagt cgttatttag gttctatttt
    22081 agcaactgtt ttacttggaa tggtatttga aggaaatata acatcagaac aatttcagat
    22141 attaggttat gttatggtgg tcttaggtac aataagtttt ttattaaatt ttatattaaa
    22201 gaaagaatta agaaaagtag aatagtggat ttatggattt aataagtcta ataactgata
    22261 agtttaaaaa tattgtttgt aaaacataac tggtttgata tttattaatt ctaagttttg
    22321 aagcatagag tactcgataa gtatacaata ttttgaatta aaattaagca gaaatttgaa
    22381 ctagtctttc aaatctctgc ttaatttagt agttaaattt ttttatagag aaaataagat
    22441 ttgacactta gataaaaaat cattgcattt tcaaaattct ttaaatcaaa cccaagtatc
    22501 tcattaatct tttccaatct atatactagc gtgtttctat gtaaaaacat actagtagat
    22561 gtcttagaaa tatttaaatc attatttata aattttattc ctgtatatag aagttcttcg
    22621 tcaatagatt taatcttatt aaaatctatt aatttattga gtatattatc ctcattaatt
    22681 aaatctaaag ttttatatct aataaggtca aacaaattta aaaacttatt ttcagcattg
    22741 tcttttataa tatgaggtag taaatcagaa cataactgta atctaaaata aaaatcagct
    22801 ttactatcta attcaccaag ataaaaaata taagtacttt gataagtttc agtttcaatt
    22861 atattttgtg tagttacatt aatatctaaa ttatatttag aatttactat aaaaaaacca
    22921 ttgtgttgta ttatatttat atcttcaaat aaattataaa aaatattttt taggtattca
    22981 aagtctatat gagattggta aattaaataa aagttgcctt ttaaattatc aagattattt
    23041 ttatctagaa aatcaataac ataaatagca ttattgaaag taactaaatg gctactagca
    23101 tctaactttt ggttttcata gggatagata tttatttttt tattaagctg tctttctaaa
    23161 aaatctttta actcttccat aaagtctcct taaaaataag attatattct tatcttatag
    23221 ctaaagtagt ttctttgtca aatatgtgta ctctactatt gtcaactgaa agtgttaagt
    23281 catcaccaac attaattctg tttttactgt caaatctaat tgttatcgta ttttcgccta
    23341 gttttaagtg agcataagtt tctgctccca taagttcttt tatttcaatt cttgatttaa
    23401 aacaagtatc tggattagat ttaataaaag tatcttctac atgaatatct tctggtctga
    23461 tacctataac aacttcatta ttaatataat tgtcttttat taaatcatag tgtttttctt
    23521 tattcaattt aagcttaata tgttcatttt gtgcataaat tttatcattt tcttctatta
    23581 atattacatc aataaaattc atctgtggtg ctccaataaa tcctgcaaca aatatattag
    23641 ctggataatc gtatacatct tgtggagttg caatctgctg aactactcca tccttcatta
    23701 ctacgattct atcagccata gtcatagctt caacttggtc gtgtgttaca tagataaatg
    23761 tagttccaag ctgttggtgt agcttagtta tctctgttct catagcagtt ctaagttttg
    23821 catctaagtt ggatagaggt tcatccatta agaatacctt aggatttcta actattgcac
    23881 gtcctaaggc aaccctttgt ctttgtcctc cagataatgt tttaggtttt ttatttaaaa
    23941 gaggtagtaa gtctaatatt ttagcagctt ctttaacttt tttgtctatt tcatctttag
    24001 gcaattttct aagtttaagt gcaaatgcca tgttttcgta aacagataag tggggatata
    24061 aagcatagtt ttgaaaaacc atagctatat ctctatcttt tggttctata tcattgacaa
    24121 gtttatctcc aatgtatagt tctccttcag agatttcttc aagaccagca atcattctaa
    24181 gtgtagtcga tttaccacaa ccagatggtc ctactaaaac gataaattct ttatctttaa
    24241 tgtctatatt tatatttttt actgcgttaa atccatttga gtaaagtttt gatatatttt
    24301 ttaaaattac ttctgacata atattctcct tgatatttaa attttaatga tatagtatca
    24361 aattttttta ttatattaaa tgtgtaaaat gactaaatgt atttgtttaa tatgtatatt
    24421 taatttttag caaagaataa aactaacagt aaaatatatt taaaaaatta aaaaacaaaa
    24481 ttaaatttat tatcaaaaaa tcattttatg atatgtttta ttttttatat tacaaataga
    24541 gaataatgaa ttatactaga aagtaggagg tgaaaatatg agcagaattt caatttttat
    24601 ggagtcatgt aatggcaaat ttccaaatga aaattcaatg ttgtcagttt cctttataaa
    24661 aaatatttta aatgcagaaa acaaagattt tgctaaaaat atatttaact atggggaaga
    24721 aagaaaaaac aataagcaaa taaaggatat aaatacagcc atctacatac caaatcttat
    24781 aagaagcaaa aatgagttgc ttgttgatgg agatataata ttcaatataa gtttttataa
    24841 ttattctatg ttcagtaaat tatacaatgg aattttaaaa gtaaaggagc tagaatataa
    24901 aggttataga tttaaaataa aaaatataaa aatacataaa gaacacgaga taaaagaaaa
    24961 tggtgttata tttaaaacta tgtctcctat aatagtaaag aataaagaag gtaaatattt
    25021 agatgtagaa gattcaaatt atatagaatg tttaaactat atagctaatc taactctgga
    25081 gagtataaga ggtagtgggc ttagaaagcc attggaattt atacctctta acttcaaaaa
    25141 aagagtgtta aaagaaaaga ttcgtggatt taaagtgaga gaatattact atataaatgc
    25201 ttatgcagga acattttttt taaaaggaaa tatggaagat ttaaatgcac tgtataaaat
    25261 gggaattgga tatagaagga cggaaaatgc aggaatggtt gatatattaa agtagcgagg
    25321 tgagagaaat aaaagctaaa ttttatagat caggtgatat tgtaagagat attggcattg
    25381 tttactttta tgaattacta atggatttaa aaaacgaact agaaaaaaat gattataagc
    25441 taaaatttac ttgtgagtta aatagaaatt atttatcttt agaatctgca gaatatatag
    25501 accctaagta tatatctgat tatatattag aaaatcaact ttttaaggta tttaaaaatg
    25561 ggaaaaagga aaccttagaa aaaatattta gtaatataga taatttaacg tatgtaaatt
    25621 ttattgaaga attagataaa tcttctgcaa ctgagaaaca aaaggaaggg attaaaaaag
    25681 attttaaaaa cagaagattt ccatatgtta gaaatagtgg taagtatgga ttaaacacga
    25741 gtttagaaaa tatggaaaca aatgtaaaaa gaatagtaga tactgtaatt aaatcaaata
    25801 tagagataga tgaaatagat aaacttgatt tgacacaata tgaagaaaaa gatagtgtat
    25861 gtaatgtttg taatataaat aaaacaacaa aactagatat tgatttaaga gagcgaaagg
    25921 actctaagta taacttttta tttagaggtg cagaaaaatc aggctttaaa agaagtggac
    25981 aagttgaaag taatatttgt tttgagtgtg aatttttcaa cttaatgtgt cttttatata
    26041 taaatttaaa aagaccaatg gtttttgcat atacgaatga tttaagggaa ttagcatttt
    26101 taaatcataa gataatgctt aaaagaaaga tgtattcaga taaatcattt tataaaaaac
    26161 ttttgcatga aaaaataagt tcgattagac tatataggtt tgatattgat acgaataaag
    26221 gtataattct aaagtttgac tctattatag agtataaaga attattaaaa aaaattgaac
    26281 ttatagatat tatagataat tataattttt caagagagcc agggaatact agaaatttgg
    26341 gtaaaaaaat gattgataat gggaatttat caaatttaaa agaattatta cttgataata
    26401 taagtgtttt aaggcaacta actggaactt ctcgagaaat ggattttgta tctagctcat
    26461 ataatatcgg tttatatata aaattgtgtt ggattgtaga tggaggggag gagtataaaa
    26521 tgaaaaacta tatggaaagt aatttaatat attcaagagt aggtaaagac ttatttaata
    26581 aaatgacaga tgaatccaga agaaaaaatt tttctatgaa agtgattcaa ctacttaagt
    26641 ctaatgatag aactcagcta tttcaaacta taatgcatgt gttagtttct aatggaatct
    26701 taataggaga agggtttgta gaaggtctaa tgcagagttc tgaattagaa ttaaatacga
    26761 atgtaggtct atttattcaa gaaatcatga aataaaaaag ggggaaaatt taaaatgaat
    26821 gaaaaaagag taaaaggagt aacattaact atactgacag aaagcccagt tccactttca
    26881 tatgaccaag ggtatggaaa ttatacacca ataaaaaaag aacaatatag agaaaaaata
    26941 catgcaaaaa ctagtatagc cacaataaca tatgatttaa gaagaatgct ccaccaagaa
    27001 tatggttgga atttaagtaa tattattttt gggaaaaaag gaaatatata tccatctatt
    27061 aagcaaaatg taggatgtgt agatgaaaat ggattagaga ctgatgtatt tggttatttg
    27121 ataccactta aagataaagg ttctataagt aaggcttctc cgcttagaat aatacctttt
    27181 aggtcattaa attcatatag aggttcaaca cagttgataa caaatagagg ttttttaagt
    27241 agcgaatttg gtagaaaata ttatgatgag aaggaagaaa atgaagtact tagagatgaa
    27301 aactttccaa catcacaagc attagcaata gaagagactt tatcagatta ctacgtttat
    27361 acaataacat tggagcttga tagaatagga gtagtagaag tagaagatgg aaagttgctt
    27421 ttaccagaag aaagaaaatt tatgagtaag gagttaagag aaaaagcagt aaaagatata
    27481 ttagatgcaa ttactgtgtt tacaagaaac ataaaacatt catcagtact gttaaaacca
    27541 cttgcagtaa tgggtggagc ttttgataaa gttgtaccgt ttttttggga tgatgtagat
    27601 tataatgctg atagtggtga gataaattta gagggagtca tagaaactat agaaagttat
    27661 agccttaaag aaagtaacac aatacttgct atcaatgaca aattaaaaat ttctaataaa
    27721 aaggaattaa ataagtttaa tttaagtaaa taccctgtaa aagagattaa aaatctagca
    27781 gatagattgg aaattggaga agataatatg tggtatttaa aagagtagga ggttttttga
    27841 attatgaaag tattacaatg taaacttaag caaccaactg ctcactatag agacccaaaa
    27901 gtatttcaaa atgaatatat aagtacttta aatttaccat ctaagaccac aataatggga
    27961 atgataacgt atttatgtga tagaagatta aactctgata tagatatagg aataattgga
    28021 acacatcatc atagagaact agaattttca agaggagaga acattgattt ttggaatgaa
    28081 tatacaaata tgagaaaagg caaagataaa gaaaagtttt tgcttcaagg aaactactat
    28141 gattactata aagaacataa agctcaaaat agcatattaa actatgaagt tcttaaagaa
    28201 gtagaactga ctatatttat ttcttgcaag gatgatgaag aacttgaatt tattaaaaaa
    28261 aagcttgaaa gtccatgtaa atatgcaaat ttaggaagaa aagaagattt tgtaatacca
    28321 agtgaaaaag ggtgttttgt gaaagaagtt gagttaaaag aggttatgcc tatgaatact
    28381 cgtgatgcta taaaagaaaa tataaaatta aagaatacat atgtaagagt tgacttaaga
    28441 gataaagaga atgtcgagac tataataaat caaggtgtat tgattgcatt accttataaa
    28501 tataaagatt tagaagcaaa tagagatgat agagtttatg agttttgcca ttatatatat
    28561 gttgataatg atggtatata tcctaaaaat attaaggtaa atgtatgtaa tgtatgtatt
    28621 gatacaaaag aagtttttac atggctttaa attattttga aagataggtg tttatcatga
    28681 aaaatatcat gcaaaaagaa atattagcta agtcatataa tttaagaact aatcaacaag
    28741 aacaaacatt agttgaacat attaaggatt tatttgaagt attagaatct atattagaac
    28801 taaatctata ttcagataaa gatgtagaga tactaaaaat atgttgtgct ttgcacgatt
    28861 taggaaaaat taattcaatt atgcaacaaa aaatggagat taataataaa atatcgtata
    28921 gttgctcaga agaagagagg aaaaagttag agtctgacaa aaaatcttta aaaaagatag
    28981 caagacacaa tatattttct ggagcatttt taaaagatat attagaaaaa atgaacttgt
    29041 tagaagaaga taagttatat atatataaaa gtataatgtt acatcatgga aactatgaag
    29101 attatatgag gttaagtact agtaaagttc aagaagaaat ttatgattat attgaaaaag
    29161 gcattttaga aaaagaagag tttaatctga aagatataga aagttatata aatgacattt
    29221 taaatgtaga ttttaaattt ggtgaagatg tattggatta tgattacata gataaattaa
    29281 gtgaaagtat gtttatagac agtgattata acaatggtca aatagatgat tcagttttaa
    29341 attatagaaa gtttaagtat attttatata aagggatgct gaacttaata gaccatagtg
    29401 catcaagtag acagaaaggt ataaaatttt ataacgattt tactgatgaa gaaattgatg
    29461 atatgatttt aaatgagata tataagagtc agggtaattt agaaaaaaat aatatagagt
    29521 tcaatactat acaaaaaagg ttgagagaac tttctggaag aaatgttctg actgttgcat
    29581 ttacaggttc aggaaaaaca gctgcagatt atagaaagac ttttaaaaga aaattctttt
    29641 tagtgccaaa taaaatatca gcagaaagtt tttatagaaa aaatattttt caaaataaaa
    29701 atgatttaga tacaagaagt agtaatgatt acattggatt acttcatgga gatataaact
    29761 tatattcaga gaatgaagat aatggagaac atgattttgt attgacatta agggatatag
    29821 atttgagtat aaatttttgt aaaccttgtg taattgcaac agttgaccag ttattactta
    29881 gtatgtttaa atttccggga tatgaaaaaa tattcgcagc cgtaaaaaat gcatctataa
    29941 ctgttgatga agtacattta ttggaaccaa agatgttttt gattttgata tattttatgc
    30001 agtttgcatg caagtactta gatgttgatt ttcacttaat gacagctaca cttccaaaat
    30061 catataaaga gcaaatgata aacaagggaa ttatttttca agaagaaagt aatgaaaatg
    30121 ttactgataa aggagaaata gtttttattg agagcaacaa ggaagaagaa atttgtgaag
    30181 gtaaagatgt aaaggtttct tttataaagg aaaaagaaat caagtcaata gttgaaggtg
    30241 ctttagaaaa taaacaaaaa attcttataa taaagaatac tattgattca gtcaatagaa
    30301 catatgagtt tttaaaagaa aatttatctg ataagtatgg tggtgtagat atagatgttt
    30361 tacatagtag atttaagttc aaagataaaa aagagaaata tagtaaaata ttaaatggag
    30421 agggtgatat ttggatatca actcaatcag tagaaatatc cttggatttg gatttcaata
    30481 taataataag tgatttagca actatggata gcttaataca aagaatgggt agatgcaata
    30541 gaaataataa atatgaatat ggaaactttt atattctacc tagtgaagat aaaatttatg
    30601 ataacaaact taagaacaca actaaaaaca tcttaaagga catattaaaa actaactcta
    30661 tttttacaat ggggattaga aaaactattt tggatgatta ctatgataat agtgtagtta
    30721 aaaactattt tgaagagaat tttataagtt gtgataaaga aattaagaat atttatggta
    30781 taaataaaga aatttttgat ggattggatt taatttttaa ttttgaacct tacaaaaata
    30841 ttgttgatag taagaaggaa gcagctaaaa tttttagaga tgttgatgtt agttacaaaa
    30901 ttattctgga agaagatttt tataaggaag atagggagtt acaacaagat tcgattcaag
    30961 tatcagggtt tatatttaat agattatatt attatggatt aataagcaaa gtagaaggat
    31021 acatggtact aaaatctagt agtaagtttg agtataatag tactgtaggc ttaattttga
    31081 agtgagcata tgtttaaata taatatacct ttagacattg aaaatacaaa aagtactact
    31141 gtaagcccac tgtaaaaact attttttaaa aggaaaaata agttgtgaga tagagtgtct
    31201 acaagtgtat atagataata aatttgaggt ttaagattaa ctaagtgggt ttcaggtaca
    31261 tcgcgaaaat aattataagt tggagatttt gtatgtttaa gattaactaa gtggatttca
    31321 aatattgata tagtagatat aatagaagtt ttattattag gtttaaaatt aactaatcgg
    31381 atttttaaat attctgacta ttatataagg atattattag cttgaattta aaaccaataa
    31441 tataaaacaa caaaatatta tatttatgat ataataaaag ttatggaaaa acaggaatat
    31501 tactatagag aagagaggta aacttatgaa taattggata gaagtaacta taaaaacaac
    31561 aacagaagca gttgaaccta taacgaatat actatatgaa caaggagctg gaggagctgt
    31621 aatagaagac ccaaaagact ttttattcca aaagaaaaat gaactagact gggattatgt
    31681 tgaagaagaa gtatttaaaa agaatgaaga agatgatgtt cttataaaaa cttatgtttc
    31741 tgaagaaaaa aatgtgatgg aatttgtcga aataataaag caaaaagttt taggattaaa
    31801 agattttggt atagatatag gtgaaggaag tgtatcattg gaccaagtaa atgaagctga
    31861 ttgggcaaat gcatggaaag catactacaa accaacaaaa gtaggacaaa gggtagtagt
    31921 aaagcctaca tgggaagatt atgctatgca agatggggat ttaatcatag agcttgaccc
    31981 tggaatggca tttggtacag ggacacatga aactactagt atgtgcatta gagaattaga
    32041 aaagtatgtg aataaagatt ctaaagtatt tgatattgga tgtggtagtg gaatccttgc
    32101 aatagcagca gctaaattag gagcaaaaga agttgtagct gtagatttag atgaagtggc
    32161 tgtaaaagtt gcaaaagaaa atgttcttga aaataaagtt gaaaaaagtg tatctgtaat
    32221 gcatggtaat ttaactgatg taataaaaga taaagcggat gtaatagttg caaatataat
    32281 agctgatata ataaagatac ttgcaaaaga tgttcaaaac tttatgaaag aagatgctat
    32341 atttatatct tcaggtataa ttttggataa agtagaagaa gttaaagaaa gcttaattga
    32401 aaatggattt gaaatagttg aagttcaaaa attaggtgaa tggtcagcaa tagtttctaa
    32461 gcttaaaaaa taagggatat ggtgacgtat aatggataga ttttttgttg aaaaaaataa
    32521 tataaatcta caagataaaa cttgcactat agaaggtgaa gatgtaaaac atatttcaaa
    32581 agttttaaga tgcaaactag gtgagaaact tgaaatatgt gacaagaata ataatgagta
    32641 tatttgtgag ataatgaata tagataagag tatagttaat ttagagattt tagaaaaagt
    32701 tgatataaat agagaatcag aacttaaagt aaggttatac cagggacttc caaaagctcc
    32761 aaaaatggaa atgatacttc aaaaattaac tgaagtgggt gtagaagaaa taatattagt
    32821 tcaaactaag agaagtgttg ttaaagttga tgacaaaaaa gaagataaaa aatttgaacg
    32881 ttgggaaaga ataatttatg aagcagcaaa gcaaagcaaa agaggtaaga taccaaaatt
    32941 aagaggtgtt ttaagtttta aagaagcttt agaagatatg aaaaaaaata atgtaaatat
    33001 ttgcccttat gaaaatgaaa gaacagtttc aataaaacat gcactaaaaa aatgtgattc
    33061 taatatagat agtgttggaa tttttatagg tccagaaggt ggattttctg aagaagaaat
    33121 agagcaaata caaaaaaata attgtaatgt tgtatcattg gggcctagaa tattaagaac
    33181 tgagacagct tctgttgtag catcaacaat agctttgtat gaactaagtg atttaggagg
    33241 agaaaaataa attgaaaaaa gtagcttttt atacacttgg gtgtaaagtt aatcaatatg
    33301 aaacagaagc tatgctagaa ctatttgaga aagatggtta tgaacaagtc aatagtgaag
    33361 agtatgctga tgtttatgtt ataaatacat gtacagttac tcatatgagt gatagaaaat
    33421 ctcgtcaata tataagacga gttaaaaaga agaatccaga tgcaataata gccgttgtag
    33481 gatgttattc tcaggtatct ccagaagaaa ttttggatat agaagaagta aatttagtca
    33541 tgggtacaaa tgatagaaga aagattgttg aagaaattaa aaagataaat tcaagtaaga
    33601 aagttagtac agttgatgac ataatgaaag ttaaagcttt tgaagaaata gaaatcagcc
    33661 aaactaatgg aaagactaga gcatttatga aaatacaaga tggatgtgac agattttgta
    33721 cttattgtat cattccttat gcccgaggac gcgttagaag tagagatatt gatagtatag
    33781 tcgatgaagt gaaaaaattg gctaataatg gctataaaga ggttgtatta acaggaatac
    33841 atgttgcctc ttatggaaaa gatttaaagg atagggatat taaacttctt gatgtaataa
    33901 aacaaataaa ccagatagaa aaaatagaaa gaattaggct tagttctgtt gaaccaattt
    33961 tatttacaga tgaatttgta aatgaagttt taaagatgga taaagtttgt cctcattacc
    34021 atttatcact tcaaagtgga tgtgatgaaa ctctaaaaag aatgaataga cgatacacaa
    34081 ctttagagta caaaactatt gtagatagat tgagaagtaa aatgccagat gtggcaataa
    34141 ctacagatgt aatagtaggt tttcctggag agacaaatga agagtttaaa aaaacgtacg
    34201 agtttttaaa agaaatagag ctttcacaaa tgcatatttt taaatattca ccaagaaaag
    34261 gaactccagc tgcaactatg gaaaatcaag ttgaccctca aatgaaacac tttagaagtg
    34321 aacaactttt aaatttaagt aaagtaaatt ttaataaatt tgcaactaaa tttatcggcc
    34381 gtgagctgga tgttttgttt gaacaaaata tagaaggcaa taaatatgag ggattaactt
    34441 caaattatat aagagttgtt gttgaaagtg acaaaaatat acaaggtcag atattaaaag
    34501 tgaaaataaa tgatgtaaaa gatgaatatg tagaaggaat tttgctataa catgtagaaa
    34561 ttataatatt tataagatag gaggtggaaa gaatggattg tattttttgt aaaatagcga
    34621 acggagaaat accttcaact aaagtttatg aagatgatag agtcttagct tttaatgact
    34681 tgaacccagt tgctccatat catatattag ttgtacctaa aaaacattat gatagtttaa
    34741 ttgatattcc tgataaagag atggacattg tgtcacatat tcatgttgtt attaataaaa
    34801 tagctaaaga gaaaggtttt gaccaaactg gattcagagt cataaataat tgtggaagtg
    34861 atggagggca agaagttaaa catttgcact atcatatttt agctggtaaa aagcttccta
    34921 attacgaagc gggacaaaac taaactaaat tattgaaaaa ttgagttcct tcttgataaa
    34981 aatacttgat aaaagtaaaa atttagttta taataaacaa agtgttaggt tgtaatgcat
    35041 attatgcaaa aagaacatag ttacagtccc agcataattg cgctattgga gggagggaaa
    35101 aagaaatgtc agaagtaaga gttagagaga atgaaacatt agacagcgct ttaagaagat
    35161 tcaagcgtca atgtgcaatg tctggcatca tgtcagaagt gagaaaaaga gagcactatg
    35221 ataagccaag cgttaagcgt aagaaaaaag ctgaagcagc aagaagaaaa aacgctaaga
    35281 aatagtagta aataagaata aagaggtgaa ggggatgtcc cttaagcaaa agttacaaga
    35341 ggatttgaaa tcttcgatga aaaacaaaga tacagtgaga aaatctgtag taactttaat
    35401 aagagcttct ataaagcagt atgaggttga taatagggtc gagcttgatg aagatggaat
    35461 tatcgatgta atagcaaaac aattgaagca acgtagagat gcattagttg aatttgaaaa
    35521 agctggaaga gaagacttaa taaaagaaac agaaggtgaa attgaagttt taaaagagta
    35581 cctacctcaa caattaagcg aagaagagtt agaagaaatt gtaaaatcta ctatatccga
    35641 agtaggggca acttctatga aggatatggg aaaaataatg tcagttatcc aaccaaaagt
    35701 taaaggaaga gctgacggaa aacttataaa taagttggta aaacaaaact tacaatagat
    35761 tgatatagcc tagagagaaa tctctaggct ttttatagtt tttgggaata acgagtcata
    35821 tttgagcata tctataatca aagaaaattt tgaaagaagg ggaaaaattt gattatatca
    35881 gtaaaagaga ttaggaattt tttgataaac atatttatac cactaataat aggttactta
    35941 agtagcatct taacaatgat tatcagtgga acagatattt caacctatta tttacaactc
    36001 acaaagccaa gctttgcacc accagctttt atctttccta tagtttggac tatattgtac
    36061 atattgatgg gtatatcttc atacctaata ttaagaaagg gttacaactt acctaaggta
    36121 agagatgcaa ttttttatta tggattacaa cttgtattaa actttatttg gagtatacta
    36181 ttttttggat ttggacttag atttacagct ttgatagata taataataat gattttggtt
    36241 tctataataa tgatttcgaa gttttctaaa atagataaga gatctggata cataaatttg
    36301 atttatttaa tgtggcttgt ttatgcagga tttttgaact attttatatg gtttattaat
    36361 aaatagaatg taaagatttt gtaaattacc ctgtactttt aataatatca taacaaacga
    36421 aaaataaata gactattaaa aataatcatt ttcatataca ataatattaa tttaaataca
    36481 ataaatttat aaaaattgta tttaaagatt aacatattaa agtattagaa ttggaaaggc
    36541 ttgaagggag aggtttttat gcaaataata gtatctggga gacaaatgaa attaactgac
    36601 ggaataaaag gatacgtaga tggaaaatta agtaggttag aaaaatacct tgatcctgaa
    36661 tcagaagtga aagtaacagt aagtgcgaaa aaggatagac aaaaagtgga ggttacaata
    36721 attcctatta acggtcaaat aataagagca gaagatgtag aagatgactt atatgcagca
    36781 attgatattg tttgtgacaa attaagtaga caagttgtca aatataaaac taaagttaaa
    36841 gacaaagttc aaaataataa gagcataaga tttgaaaatc ttgactttat agataatagc
    36901 agtgaatttg atgattatga ttatgatgat gaagaagatg aaaatatagt aatagaaaga
    36961 agaaagaaat ttaatgtaaa acctatgagt tctgaggagg cgatacttca aatggaacta
    37021 gtaggacaca atttctatat gtttagaaac caagataatt ttgaaataaa catagtgtat
    37081 aagcgtaaag ctggtggata tggtttaata gaacaagatt aataaaaatt gtatacgttt
    37141 agattttata taagaaaaaa taaaaaggta atcatgttga ttttttatca atatgattac
    37201 ctttttattg tttaaataat atattagata ttaggtattt attatataaa aattttaaaa
    37261 ctaatctgtt acaacatcaa taaagtgcat aaatacctat ttcaagcata aaattaatta
    37321 agggggtaat gcttatgtta gaaatatcca ctgatttaca agtaaatcaa ccagttatta
    37381 ctgttacaag taatacgttt attagtatag aaaattatct atcaatatta gaatatgaag
    37441 ttgatttaat aagaataaag actaaagtta aaacaattaa aatatctggg gataaacttt
    37501 cacttaagta tattactgat agtgagatag gtataaaagg gattatctac aatgttgagt
    37561 atgttgatta ggaggagata cacttgataa gttttataag gggatactat gttatagttg
    37621 tggaaggagt aggactcgaa caatttttaa atcatcttat aagaaatggg ataagtgttt
    37681 acaatgtaac tagaataaaa aatactaaaa tggagtttca tatagataga caagatataa
    37741 aagaatttaa gaatgtttat agaggaagta agtttgatat aaaggtaaag caaaaaactg
    37801 gggtcccttt tattatcaaa agagtttata agcacaaagg tatgtggatt tgtgctcttg
    37861 tttctttatt tttattgatg tctacatcgc agtttgtgac agatgtatat atacaatctc
    37921 ctgaaggtat aaaaaaagaa gctcttagaa atgagcttta taaggttgga gtaagaccag
    37981 gagtctataa gaagagtatt gatagaaaag aagttagaga ccatatgatg tcaaaattta
    38041 atgatgtggc atatttatct atcaatgtaa aaggaacaaa tatatttgtt actgttacaa
    38101 aaaaggcaga atccctaaaa tctacagacc aatcaaatta ttgtaatgta attgctttaa
    38161 aaaatggtat aatagaaaag gtaatcccta gaagtggtaa atcagttatt aaatctggag
    38221 acatagtcca aaagggagat gtcttattaa atggagctaa taccaagtca ataccagaag
    38281 tatgggcaag tactttttat gagtccacaa aaaaggcaag ttatgtagat actgtaaata
    38341 aaaaaactgg agaaaagaaa aatatatata cactaagttt ttatgataaa gagtttacaa
    38401 tgagaaaaaa tataaaatat aaagattatg ttgttgaaaa taaagaaaaa aaactatcaa
    38461 taggaaacta tacttttcca ataaagataa aaacgagtac tttttatgaa acaaaaaagg
    38521 ttgaagtaaa aaggaataaa gaagagttaa aaaaagaatt atctgaaaaa gctttaaaag
    38581 agttagaata cataatccca gcttcggcta gaataataga tgtaaaacat aactttaagg
    38641 ttaataaaaa tatgttagaa tatttaataa cagttcaaac tagtgaaaat attgctaaaa
    38701 tttatcctct tagtaagtca gaagcagaaa ggtttataaa ggaagaaagt aaaccagatg
    38761 aaggtgaaga ggaagttcct tccaatccag aaaaaagacc attaaatgat ataagaaatg
    38821 agtttgatga ggataataag gataataaag accaaaacaa ttctgatgaa aataatagta
    38881 atcaaaatag taataatagt caaaataata atagcaataa caactaataa aattaaactt
    38941 aaaatcttag gaggactaat tcaattgata atacaaaaga aatttacaat agcagatggt
    39001 aactttgaaa gagagttatt tggcaacttt gatgaaaatg taaaattaat agagaagact
    39061 ttaaatatag atgtaatact tagagaaggc aatataattt taattggaga agaaaaaaat
    39121 gtagattctg cattaaaact tatgaatgaa ttacaccaaa ctgtatcaaa tggtaagcat
    39181 ttagataagc agagtatttc ttattcatta tctcttttgc ttgaaggaag tgaacaaaaa
    39241 ataaaagaac ttgaggggac aatcgtcata actcaaaggg gaaaagctgt tcaacctaaa
    39301 actttaggtc aaaaagaata tataaaactt atagaaaata atgatataac ttttggtgtt
    39361 ggacctgctg gaacaggaaa gacttatctt gcggttgcta tggcagtaaa agcttttaaa
    39421 agagatgaag ttagtagaat tatacttaca agaccagctg tggaagcagg agagagtcta
    39481 ggttttttac caggagattt aaaggacaaa gttgacccat atttaagacc actatatgat
    39541 gccttatttg aaatgttggg tgctgataaa tttaataagt atttagaaag aggtactatt
    39601 gaggttgcac cacttgcatt tatgagaggt agaacacttg ataattcatt tataatattg
    39661 gatgaagctc aaaatactac ttctgaacaa atgaaaatgt ttttaactag acttggattt
    39721 ggctctaaag ctgtagttac aggagatgta actcaaacag atttaccaca aaataagaag
    39781 agtggtctaa tacaagctac agagatcctt aaaggtgttc caggtattgg gtctataatg
    39841 ctaacagata gagatgttgt aagacatgag ttggtacaga gaataataag agcttatgag
    39901 aagcacgaca aaagagaaga atttaaaaaa gaagaacgta aaaaagtgaa aatgcaggaa
    39961 aagactttca aaagaaagta aaagtgtagt aatatagata tatagcatta ttaatgaata
    40021 aaaagtaggt gtagtgaatg gatttgatac tagatgatag acaagataaa ttagaagtaa
    40081 gtgaggagtt aattgaaaag ataaaggata taatcataga atgtttagat tatgaaggat
    40141 atgatgataa ttatgaagtg agtctttctt ttgtagataa taaagagatt catgaattaa
    40201 atagagaata tagaggagtt gatagagtga ctgatgtttt atcatttcct cttttaagtg
    40261 atgattttga ggatgttgag ttagaagaag aatccctagg tgacatagta gtatctcttg
    40321 aaagagcctt agaacagagt atagagtata accatagttt tgaaagagaa gtatgttttt
    40381 taatatgtca tagtatgttt catcttttag gttatgacca cgatacagat gaaaatacaa
    40441 aagaaatgag agaaaaagaa gaacacatat taaataagtt aaacataaca agggagtagt
    40501 ttttatgaaa ccagaaaaaa ctcgtcaagg tataattaaa gcatttaatg cagctataga
    40561 agggatattg tatacattta aatttgaaag aaatatgaaa atacattatt taggttcagt
    40621 tgctgttctt ataataagtt tgttttttaa cttttcaaaa ttagaaatga taatgttact
    40681 tatgtccata tgcttagttg tagttgccga aatgtttaat acagctatag aaaaagctgt
    40741 tgatttagta actgatgaat atcatgttct tgctaagata gcaaaagatg ttgcagctgg
    40801 aggcgttcta gtggcagcac taaattcagt agtagttgga tatattttgt tttatgataa
    40861 attgacagat atatctggaa tattaattta taaaataaga gaatcagaat tacatataac
    40921 cttgatatgt atattattgg ttcttatagc tgttgtagtt gtaaaagcat tgacttctac
    40981 aggtactcct ctaaaaggag gaatgccaag tggtcatgct gccttagcat ttgctatagc
    41041 aactgcaatt acacttatga ctgaaagagt tgttgcctct actcttgcat atataatggc
    41101 tgtattagtt gcccaaagta gaattgaagg aaaaatacat accttttggg agactattgc
    41161 tggagcttta ttaggtgtgt tgatagctat attagtattt cagcttggaa tgttctataa
    41221 ttaaaataaa tgtacataaa atatttaaaa taaaacaaat ctgtgctaat taaattttta
    41281 tgatataatg tagagtatta aataaaaata acttttaatt attgtataca ggaggagttg
    41341 ttgtggataa catagaattg ttaaggttgg cagaagatgc cagacaacat tcttatgctc
    41401 cttactcagg ttttagagta ggtgcagctc ttttaactaa gagtggtaaa gtttatactg
    41461 gatgtaatat agaatgtgca tcattaggtg gaacaaattg tgcagaaaga acagctattt
    41521 ttaaagcaat atcagagggt gataaggata tctataaaat agctatagca agtgataact
    41581 cagaaaataa tgagcaaaca tatccatgtg gaatatgcag gcaggtaatt atagagtttg
    41641 gttctgatat aaaaataata acgggatata ctaaaggaga ggtgtttgag catagtataa
    41701 aggatttgtt gccaaactat ttctctggaa atgatttaaa ataaatttaa aagtataaat
    41761 aaatttaagt aattaaaaca agaagataaa aagttaggag acagttaatg ttcaaatcag
    41821 gttttgtcag tatagtgggt agaccaaatg taggaaaatc tactttaatg aataatgtag
    41881 ttggagaaaa aatagctata atgagtgata agcctcaaac tacaagaaat acaatacaag
    41941 cagtatatac agatgaagaa atgcagatag tattcttaga tacacccggt attcacaaac
    42001 caaaaaataa attaggtgaa tttatggtaa aggcagcaac agaagctttt aaaaatgtag
    42061 atttaatatt atttgtagtt gatgattcta aaaaaatagg tcctggagat agaaagataa
    42121 tagaggattt aagaagtgtt aagacaccaa taattttagt tgtaaacaaa atagaccaac
    42181 tagaccaaaa agatgagcta tttgatataa tcaaaatgta tgatagagaa ggtattttta
    42241 aagaaatagt accaatatca gcattaaaag gaaagaatac ggatacctta ataaaagtta
    42301 tacaaaatta tttagaagaa gggccaaagt attttccaga ttatatgatt acagaccagc
    42361 cagaaagagt attaatagca gaacttataa gagaaaaagt tcttcactat cttaatgatg
    42421 aaatacctca tggtgtagct gttgaaattg aaaaaatgaa agcgagaaat gataaggaaa
    42481 tagtagatgt atctgctgta atatactgtg aaagagattc acataaggga ataataatag
    42541 gaaaaaatgg taggaaacta aaaggaatag gtaagtcagc aagacaagat atagaattgc
    42601 ttttaggttc acaaataaat cttcaattat gggttaaagt caaagaaaat tggagaaatt
    42661 tacaaaatta tataaataat tttggatata atgataaata aaggtgggtt atatggatag
    42721 gaaactggat gcttcccaag atttattata tgaagtaaaa tcattaattg ataataataa
    42781 agtattggag ttaagagaat taatagagga atatcatata attgatatat tcgatataat
    42841 ggaaaattta gaggaagaca tgaaaattca attatttgaa gtacttcctt tagatatggc
    42901 atcttctata ttagaagaag gtagtgtaga gttttttatt agtattttat ctaaactgga
    42961 tgtagaacac tcgaaaaata tacttgaact tatgtctctt ggagatatgg cagacaagtt
    43021 gagtgaactt gaagaagaag aaagagaaca cataataaat cttttgaatc aagagaatgc
    43081 cgattatgta aaagagttac tattctatga tgaagattct gctggtggaa caatgaccac
    43141 tggatatatt tctataaata aagatatgac agctcttgag gctatagacc atatgagaga
    43201 agaagctgaa gaagctgaaa ctatttatta tatatatgtg gttgatgatg aagaaaaact
    43261 agttggagta ttgtcattaa gagaattaat aatagctaga gacgcaaata tagtagaaga
    43321 tttgatgagt gaaaatataa tctctgttta tgtagatgaa gatagagaag aagcagtaag
    43381 attagtatct aaatacaatt taatagcaat tccagtagta gacagacaag aaaaattaaa
    43441 aggaataata acagtagatg atataattga tgtaatggaa gaagaagcaa ctgaagatat
    43501 gtataaattt gcaggaagtt cagaacatga aagagaagtt gctgaaaaag aaaaccctac
    43561 attgagagag caaataatat cagcacttag aggacgatta ccttggttaa ttattacctt
    43621 ggttggaggg ttgttggctt cgttaatact ttctaactta gactatataa tgaatcctgt
    43681 atatgctcca ttagtatttt ttatacctgt agtaattggt atgggtggaa atattggaac
    43741 tcaatcatca tctgttacag taataacatt atctaataag gatttaaatt ttagcaatgt
    43801 ggttagagaa ggtattgtag gtattattac aggtcttttg tgtagtataa taacaggtat
    43861 tgtaatttac tttgttatga gaaagttaga cattgtatta attgtttcaa tatcattatt
    43921 tataaatatg gttttaggtg caacaatagg tgcgtttatg cctgttttat taaaaaaaat
    43981 ggatgcagac ccatctacag tttcatcacc aattatatct acagctcttg atataactgg
    44041 tatagcagtt tactttataa taacaacggc attattgtca aaaattgttt aataacttta
    44101 tgtatgattt atcctcttct tactaaaaat ataagtataa atgtaagaag ggggtttttt
    44161 aaaaatgaac aatatcagtt gggaagtttt taaaaaaaca ggtagtatag atgcatattt
    44221 atatttctgt gattgcaaaa acatacatga agaaattgaa gaagtaaggg agaaaaaaga
    44281 agacgatgat aatcctgaac acccagggga tagtacttaa agctataagg tataaggaaa
    44341 gtgacataat acttacacta tttacaagaa agcttggaaa agtatctgca atagcaaaag
    44401 gagctaaaaa aaataagagc tctttgcttt catcatcaca attattttct tatagcaatt
    44461 ttaccttaaa aaaacaaggt aatatgtaca aggttactca aagtgaaata ataaaaagtt
    44521 tttataatat atcctatgat atagaggcat tttcttatgc tacatatatt acaaaattag
    44581 ttgaaaattc tatacttgaa aatcagacta ataataggct atttatatta cttgcgcaga
    44641 ctctgtattt gtatacacag gataatacag acaataggtt tataacagca gcattcgagt
    44701 taaaattttt agactatata ggatttaaac ctatagtaaa taaatgcatt aattgtgaat
    44761 gtaaaatttt acaaaattct gtgtttaata tctacgaagg tggtatacta tgtagtaagt
    44821 gtagtaaact ttttgataat aatataaaat tagacttaac aactataagt cttatggaat
    44881 atgtattaag aaatgatatt ttaacatgta gtaaagctaa agtatcaaaa tatataacac
    44941 atgaacttga gaatattcta gacaagtatt tgaaagttta tgttgataat attaatttta
    45001 aatcattaca cgtattgcaa agtgtaaaaa ataataaggg agtggataat gatgagtagt
    45061 aatataacta ttgaaatggt tgattcagtc tttgaaagag ttccaggagc aacttatgca
    45121 gaagcaaaag aagcattagt aatgtgcaat ggagatgtaa ttgaggcagt aatatattta
    45181 gaaaataaaa aaaatacttg taattgtaaa gcaaaatctg ctaaagaaac tatggaagaa
    45241 gtatttggca aagatggaga aaatataaag tttcaattaa aagagcttct tagaaaaagt
    45301 agtgtagtga gaataatagt tgaaaaagat ggaaaaacta tgatgaacat tcctttaaca
    45361 gttggagttg ttggattagc ttttgtacca ttagctactt tagttggatt atcagctgct
    45421 gttataagta aatatcgtat aaaagttcaa aatgaagaag atggagaaat tgtagattta
    45481 ggtgaattga atgaagaaaa acttcacgtt ttgaagaata tgataactaa tgcagcaaaa
    45541 gatgtgaagg atgtagtagt tgacaaaaaa gaagatgaca aagatgtaac tgctgacctt
    45601 atgaaagaag aatctgaaaa gaataaagaa gacgaagacg aaaataaata gatataaagt
    45661 gacttttaaa aaatgaagag aagataatta taagaattat cactaacatt gacaaatttt
    45721 atagaatttg atattctaaa agttgtagaa gaactattaa aatagccttt cgtgtagacg
    45781 agaggcttta ttgttatagg agggattttt atgaattttc aagagatgat actggcgttg
    45841 caaaaatact ggtcaaagca aggttgtatc atgatgcaac cttatgatat tgaaaaaggt
    45901 gcaggaacta tgaatcctaa tactttttta agatcattag gacctgagcc ttggcaagta
    45961 tgctatgtag agccttcaag aagaccagca gatggaagat atggtgaaaa cccaaacaga
    46021 ctatatcaac atcatcaatt ccaagttata ctaaaacctt ctccagataa tatacaagaa
    46081 ttatatttgg aaagtttaaa agagattgga attgacccaa gtgagcatga cataagattt
    46141 gttgaagata actgggaagc tgctacagtt ggtgcttggg gacttggatg ggaagtttgg
    46201 ctagatggta tggaaataac tcaatttacg tattttcaac aagtaggtaa tattgaatgt
    46261 gagcttgaga caggagaaat tacatatgga ttagaaagat tagctatgta cattcaagaa
    46321 gttgatagtg tttatgattt aaaatggaat gataaaataa cttatggtga agtatttaat
    46381 aaagctgagt atgaaaactc aatgtacgca tttgaattat gtgatgcaga tatgttgttt
    46441 aatctgtttg atatatatga aaaagaagca ttaagactta tggaaaatgg ccttgtaata
    46501 ccttcatatg actatgtgtt aaaatgctct catgcattta atacattgga cgcaagaggt
    46561 gcaataggag ttagtcaaag agcttcattt ataggtagag ttagaaatat ggcaaagact
    46621 gttgcagaaa cttttgtaaa acaaagagaa gaaatgggat tccctctttt aaaggacggt
    46681 gacaaataat gaaaaattat cttttatttg aaatcggtgt tgaagaaatg ccatcaagat
    46741 ttgtaggcag tacactagaa caattaaaaa acaatcttag taaattattt gatgaaaata
    46801 gaatagcttt tgataaaata aatgcgtatg ggacaccaag aagacttact ttagtagtcg
    46861 aaggattgag tgatagacaa agtgatttag aagaggaaat aaagggtcct gctaagaaaa
    46921 ttgctgttga tgcagaaaat aatttaacaa aaccagcatt aggatttata aaaagcaaag
    46981 gattaagcga atctgacata tattttaaac aagttggaaa agaagaatat atatttgcga
    47041 ctataaagca agatggaaaa gaaacttcag aagtactaaa agacatatta ccagagacta
    47101 taaaatctgt agtattccca aaagctatgc gttggggagg aaaaaatctt aaatttgcaa
    47161 gacctataag atggatagtt gcattgttaa atgataaagt attagagttt gatttagaag
    47221 gaataatatc ttctaatgta acaaaaggtc atagattttt aggtgaaagt acaatagaag
    47281 ttgattctat agaagattat tttgaaaaat tagaaaaaaa ttatatagtt ttagaccaaa
    47341 acaagagaaa agaaatgata agaaaacaat gtatagaagt agcaaattct ttaggtggag
    47401 aagttgaatt tgatgaagat ttacttgaag aggtaactta tttagttgaa tatccaactg
    47461 ctttttatgg agaatttgat gtggattata taaaactacc aaaagaagtt gttataactc
    47521 ctatgaaaca acatcaaaga tattttccag tacttaaaga aggaaaactg ttacctaact
    47581 ttatagctgt tagaaatggt gattcttaca gaatagacaa tgttaaagct ggaaatgaaa
    47641 aagttttaga agcaagactt gcagatgctt tatttttcta tagagaagat actaagagaa
    47701 acttagaaag ctacatagaa aaattaaaaa ctgttgtatt ccaagtaaaa ctaggaacta
    47761 tttacgacaa aactttaaga cttgaaaaat taagtgctga tatacttgat agtttaggtt
    47821 tagtagatga aaaggcagat actataaggg ctgctaaatt atctaaggca gatttagtta
    47881 ctggaatggt aggagaattt gatgaattac aaggttttat gggaaaagaa tacgcaaaag
    47941 ttggaggaga gaatgatgtt gtaagtgaag ctatttttga acattatctt ccaagatttg
    48001 caggagatat acttcctaag actaatgcag gggttgcctt atcaatagct gataagttgg
    48061 attcaatagc aggtttcttt gctataggaa tacaaccaac aggttctcaa gacccatatg
    48121 ctttaagaag acaagcttta ggtatactta gtatacttat ggacagaaaa gttgctgtgg
    48181 atataaaagt acttattgaa catgctttag ataattattc tgaattagat tttaataaag
    48241 aagaagttgt tgagcaaata atgaatttct tcaatgagag agtaaaaaat ctatttaaag
    48301 atttaggaat aagatatgat gttgttgatg ctgttttaag ttctgatata aatgatgttt
    48361 ctgatatgca tatgagagct ttagagctta ataattggct agaaaaagat gaattagtgg
    48421 agatgcttac tgcatttaat agagtttcta ctctagctca aaaagcagaa agtgataaaa
    48481 ttgatgaatc tttattaaaa gaagatgctg aaattagact ttacaatgaa tttaaacaag
    48541 taaaggtaaa agttaatgaa ttattaaagg ataagaaata tggtattgct ttagatgaat
    48601 ttgcatcttt aagaccagta gttgataatt tatttgatac agttatggtt atggataatg
    48661 atgaagctat taagaacaat agacttgctt tacttaaaca agtatatgat actatgctag
    48721 aaatttgtga tttatctaaa attgtttata aataaaattt aaactattta taagaaataa
    48781 ggctgtctca aaatagagat agtcttattt ttcattgttg agatgatgaa aatatatttg
    48841 atttcataga aaaagagtgt ctcataaact aaaaagttca ttttgagaca cccatgcttt
    48901 ttgtaagtaa atataataaa ttatatttta tacttatcag ttattttgtt taaaatatta
    48961 tcatttgaat ttacccattc ataaaaattg cctgatgatg cgtccgatat tttataaagc
    49021 atttcaggaa tatcactagc tttaaaatct ccaagtgatt ttcccaatct agtcttacct
    49081 atttcaaaac tgcctccaac aaataattca aatgcatctg tagatatacc atcaacattc
    49141 ttctttttac cacataaacc tatgctacca acttgatgaa caccacatga atttaaacat
    49201 cctgatatat ataacttagg tgctttattt aaaatttctt cattattttg taatctaaaa
    49261 taatctatga tttcatgtaa cattttctga ctattttgta tgcccatttg acaaattgga
    49321 acacctatgc aagatatact ttgttctaat tgagaattac aactaatagt tttacttatt
    49381 tctaatattc tttttgcttc atttccattt aaatttaaaa tatataatcc ctctgtcatt
    49441 cctagtctaa tcataggatt ttcaacatta tctaattctt taagtaaagt acttaaatcc
    49501 tttgttaaca aaatacctcc aactggatgt atatatacag tgtataaacc atcctgtttt
    49561 tgaggtatta gtctagaatc ttgtatatct attttaactc caggtttaga gtaatctatt
    49621 tcttcaatat tgagttcaag actaccattt tctttttcta atttatagta ttctttaaat
    49681 ttttctaaga atagttcttc accaagtgat tcgaccatat atctaaccct agctctgttt
    49741 ttattttcat aatttccata atccataaag aatttaatca taccttcaac acaatataag
    49801 gcgtctttcg gctttataag ttcatcaagt tctaaagcaa ctttgggttg tttacctaga
    49861 cctccaccta agaaaactct gaaatatggt tcatcatttt tcaaagtagc aacaaatcct
    49921 aagtcttgta tactacaatg agcagtatct gtgtaacaat tagaataaga aacttttaat
    49981 tttctaggta aatggtatgt agtagcattt ttgataaaat atttatcagt agcaactgca
    50041 tatggagtaa catcaaagac ttcactaggg tcaactccag ataatggaga taatccgaca
    50101 tttctaggat aattaccacc accacctcta gtgaatatat cattttttat tccttcttcc
    50161 atgatatcaa caattgaatt tatagataaa tcgtgtaatt gtatagcttg tctagttgtt
    50221 aagtgtatct tatctaagtt gtgtttaata gctatttcat agattttatt aagttgatac
    50281 tgagataaaa ctccagcaga tactctaagt cttatcataa aagatttttt gtctctttga
    50341 gcataaacac catatccacc agaaaatccc ttatattgca ttttatttat ttctccattt
    50401 aaaaattttg aagaatattc tttaaaatta ggtagttcat ttaacaatat ctttttaaaa
    50461 ttatccatta tttcacctca ttgttagtct tgtataaaaa tatatctatt gtataataga
    50521 tatatatatt attatctagt caggataaaa atataacaga ataattctat taaaagtaag
    50581 aataataagg acatagtgag tattttgttt ttattttaca aattaagtat aatatttttg
    50641 ttttataaaa tataattaag ataaatagat aattttgtaa ttttaatata taatatttag
    50701 tagagtgtgg gagtagcact taatatttgc aggaggagtt tttgtatatg ggagaaataa
    50761 taaatgcatt agatagagcc ctagacataa ttttgctgtt atatcatgaa aagagagaaa
    50821 tgggaataac tgaaataagt aaggctatgg gagtatataa aagtactgta catagaactt
    50881 tggttactct tgaaaacaaa ggttttgtta tacaaaatgc agagaatggt aagtattggc
    50941 taggaataaa tttatatgca ataggtatgg ttgtgggaga aaaaatgtca ttgactgaaa
    51001 tagtaaaacc atatacgaaa aaattaaatc aagaatttaa cgaagtagtt aatgtatcta
    51061 tattagaaga aagagctcaa gattctccaa gaagtatcat aatacataaa gagtatggtt
    51121 caaatcaatt actttcagta aatccatccg ttggttcaag tagtgaatgt tattgttcag
    51181 cagttggtaa atgtcttatg gcatttaatg attctataga ttttgaaaaa tatagaaaaa
    51241 caccaataca caagtataca gaacatacaa ttgataattg ggatgatatg atgctttttt
    51301 tagcaaaaat aaaagaacaa ggatatgcta tagatgatga agaactagaa catggactaa
    51361 cttgtattgg agcacctatc ttagataaaa ataataaagc tattgcagcc ataagtttgt
    51421 caggtccaac tataagaatg agagaagggg actttgagta taagattaaa agagttatag
    51481 aaactgcaaa aagtatatct gaacttttta gatgattttt aatctcgata ataaaaatag
    51541 gctatattta aattgttatt tgtaaatatc agtttaaatg tagcctattt ttctttaatc
    51601 taaaattaaa tattcttaaa atttaaaatg ttattgaaat atataagcga atgtgttatc
    51661 ttttaattaa gcaataaaaa tagaacaagg ttctatacta tagaacaaaa ttgatgcata
    51721 tatagtattt atacaaaata taaatattat atatttaaaa ttaagtaaaa atattacata
    51781 aaggggagat ttgagaaatg gaaaaagtca agttgagtgt tgaaagatgt aagggatgct
    51841 atctttgtat tgaagcttgc aaaaaagatg caataagtct ttctgactac acaaataaaa
    51901 aaggctttgt aactattaaa gtcgatgaag aaaaatgtat tcaatgtggc gcttgttata
    51961 caatgtgccc tgatttagta tttgagatat tataaattat ttttattaga ggggagaaaa
    52021 ggtatgcaaa gtttattaat taaaggaaat gaagctttag cagaaggtgc tatacgtggt
    52081 ggctgcagat tttatgcagg ttatccaata acaccacaat cagaaatatt agaaatgatg
    52141 gcatggagac aaaaagaagt tggaggagta tttatccaag gtgaatcaga aatagccagt
    52201 gtaaatatgg ttatgggagc aagtgcactt ggagcaagag ctatgacttc ttcatctggt
    52261 ccaggatttt cattaaaaca agaaggtatt gcatattggg tatcagctgg tttgccagca
    52321 gttgtagcat gtgtgcaaag atatggtata ggtgatggat ttataggagc tggtcaagat
    52381 aattattggc aagcagtaaa aggcggtgga aatggagatt atcatttaat cgtgttagcg
    52441 cctaacagtg ttcaagaaaa tatggatatg gcatatgaat cttttgaatt ggctgaaaaa
    52501 tatatgcatc cagtacttat actttctgat gctacaatag gtcaaatggt agaaccttgt
    52561 atagcaccac caatgaaaga atacgatatg gataaagctc catgggctgc aaaaggaaca
    52621 cctgtaggag aaaaaaataa agtaataact gatataacat attttgaaga ccaagataca
    52681 tggcaaaaag ggtatataga aaaaatgaga aaagttcaag aagatgaaca aagatgggaa
    52741 gagatagaaa tagaagatgc tgaagtggta tttgtggcat acggtatttc atcaagaata
    52801 gcagaaggtg cagttagaga tgctagagca aaaggaatga aattaggtct aataagacca
    52861 aaaacattgt ggccttaccc aagaaaagca tttaagaaat taagtgattc attgaaggga
    52921 cttgtaacag ttgaaatgaa catgatggca cagatgaagg aagatgtaat tatagcatgt
    52981 gataatagat ttccagtata ttcagtagca acagctcaat atatggcaga agtaaatcaa
    53041 ttaatagatt ttgctcaaca gattatagat ggaaaagcta agcaagagga ggtattttaa
    53101 ttatggctaa gaaagcacca gcaataatga tgtgtgaaaa tatgttttgt ggaggctgtg
    53161 gacactctat agtcgataga attattggag aagtattaac agaaatggat atatgtcaag
    53221 atacaattat atgttcagat ataggttgta atcatatgtt tcaatttagt atgaatgtag
    53281 atgttgtcgt tccacctcat ggtaaaatgg gtgcagcaat ggcagctatg aaaagagtta
    53341 gaccagataa atatatattt acaatagctg gagatggtgg agcttatgcg atagggcttg
    53401 gggaaactat gtcagctgct actagaaatg ataatgttat attccttgtt atgaacaata
    53461 atatgtttgc aatgacagga gggcaaatgg ctccaactac tatagaaggt caatatacag
    53521 cttctactcc agcgggtaga aattacgcag atcatggttc aacatttgat gttgtaaaag
    53581 ttatgggaaa tttagatata gcatatctag caagaggaac aataactaac cctaaagaaa
    53641 taaatacaac aaaaagatac atcaaaaaag ctctagaaaa acaaaaagaa aagaaaggtt
    53701 tttgcttaat agaagtgctt gtaccatgtc caacaaactg gggattaaag ccagtagatg
    53761 ctatgaagag aattgaaaat gaacttgttc caaagtatcc acttggagaa tttattgatt
    53821 agggaggaaa taatatgttg aatgaaatta tatgtgctgg ttttggagga cagggagttc
    53881 ttacagcagg aaaaattata atgtatgttg catataaggc agaaaagaaa attacatggt
    53941 tcccatctta tggaaatgag atgaggggag gaagtgcaaa ttgcaatgtt gttgtaagtg
    54001 atgaaaaaat tgcaaatcct tgtgtaaaaa atccagatat actattggga atgaacagcg
    54061 tatcagtaga taagtatgaa agtttaatga aaaaaaatgg ttatatgttt gtcaatgaat
    54121 cactagtaag taaggataga aagtacagag aagacattaa tgttataaaa gtacctgtta
    54181 ctgaaatagc acaaaattta ggcaatgaaa aagctgctaa catttgcatg ttaggtgcta
    54241 tggtaaagag tttggattta tttacaaaag aagaatttga aagtggtatg tgtgaatact
    54301 ttgaaaatca aggcaaaggt aaattcaatt ctaaaaatgt agaagcattt gaagcaggat
    54361 acgactacga atgaatattt taaatattta aaataattat tgaaatactt atttttatat
    54421 gttagtattt aattaataaa atatagaacg tcgttctgga caataggacg aagtctttag
    54481 gccggaatac aagaacgacg ttctgtatag tagaactagg gggtgttaaa ttgaaagttt
    54541 tagtaataaa tcctggtggt acatccacaa aaatttctgt tttccaagat gaaaatgaaa
    54601 ttttgaaaaa aaatataacc catacaagag aagatttaaa aaatttttct aaagtatttg
    54661 atgaatatga ttatagaaaa caactgatag tagatatttt aagtagtgag aatcatagta
    54721 taaattcttt tgacgcagta gttggtcgtg gtggacttat gaaggctata aaaggtggta
    54781 cctatactgt gagtgaagaa atgattgagg atatgagaaa tgaaataaat ggggaacatg
    54841 catcaaattt aggtgcatta ttagctaaaa ctatagctga tgaaatagga gtacaatcat
    54901 ttgtagtaga cccagtttct gtagatgaat ttgatgatgt atcgaggatt acaggaatat
    54961 cagacataga aaaagcaagt tggttacatg cattaaacca taaagctgtt tcaagaaaaa
    55021 ttgcacaaaa actaggggga aaatacgagg attataactt tattgtagct catttaggtt
    55081 caggaatatc gattgtagca cataaacaag gcaaaatgat tgatggaagt ggtggtaggt
    55141 ctgatggtcc attttctcca gaaaggagtg gtggattact tgcatatcct cttatagaat
    55201 tgtgttactc cggaaaatat actcatgatg aaatggtcgc aaaagtaagt tcgattgggg
    55261 gaatgtacga ttatttagga acaaaggaca tgatagaaat tgaaaacaga atcaaggagg
    55321 gtgatgaaaa ggcaaaactt ataatggatg catttattta tcaagtagca aaagaaattt
    55381 ctatgtatgg agcttcactt tcagggaatg tagatagagt tatcttgaca ggagggatat
    55441 ctcactcaga aatggtagtc aaaggagtag cagaaaaagt ttcatatcta gctccaatag
    55501 agatagtacc aggagagatg gaaatggagg cacttgctct aggagcactt agagtactta
    55561 atggagaaga aatagcaaaa acttattaat atttaggaga aagagtatgt taaaagattt
    55621 taataattta ttagaagagg ctaaaaagct tgaacctgtt attgtaagtg ttgctgttgc
    55681 agaagacaaa gaagtattaa aggctgtgaa atcagcaaca gatttaaaca ttataaagcc
    55741 aattcttgta ggagacaagg ataaaataga aagtatatca agagaaattg gattaaaagg
    55801 tcatgaaata ataggttgtc aaaatcccga tgaatcagtt aaaaaagctg tagagatagt
    55861 taaaaataag gaagctaaag ttcttatgaa gggattagta aatacaagtg tctatatgag
    55921 agctatatta aataaagaaa cgggtcttag aacaggaaga ttattaagtt tgcttgcagt
    55981 atatgaatta cctcaatatc ataaacttct ttattgtact gatagtggaa taaatgtatc
    56041 gccaaactta aatcaaaaaa aagatataat gacaaatgca cttcttacta tgaaaagtat
    56101 aggcatggaa aatcctaaaa ctgcgatact tactgcaaat gaaatggtag accctaagat
    56161 acaatctaca gtggatgcca acctacttgt agagatggaa aaatcgggag aaataccaaa
    56221 gtgtatagtg gaaggtccaa tagcatttga tgtagcattt gatgcacatg cagcagaaca
    56281 taaaggtatt aaaagtaata ttgctggtga tgttgattta ttagtatttc caaatataga
    56341 agcagggaat atattaggaa agtcatggct tagatttaat aaagctaagt gggcaggtat
    56401 agttttagga gcaacaaatc cgataatttt aggttcacgt tctgacacag ctgaaataaa
    56461 aataaattca atagcattag cgtgcctagc atcacaataa gaaattatta ttaaggagga
    56521 gaaagtatta tgaaatatgc aaaaagaatg gatatggtaa aagcttcagc tataagagct
    56581 tctcaaaaga aaatagctca gaaggtggca agtggtggaa ctgttatatc ttttgctgca
    56641 gggcttcctg acccaaattt atttccaata gaagagataa gtgaaataac tcaaaaagta
    56701 cttaatgaaa ggggaaaatt tgccctagca tatggaccta ctaaaggtga agatgagttg
    56761 ttagatctat tagtaaaaag aatgaaagaa gaagaaaata ttgatacaaa agcagaaaat
    56821 attataataa ctactgggtc acaacaaggg atagcattaa gtgctatgat tttactagaa
    56881 aaaggagata tagtagttac agaaaatcca agttacttag gtgcaataaa tgcatttaga
    56941 ccttatgaat gtgatttttt aggagtagat actgatgatg aaggaatggt aacagatgat
    57001 ttagataaat tattatctga aaattctaac ataaaggtaa tctatgtaat accaacattt
    57061 caaaatccta caggaaaaac atggtcttta caaagaagaa aagattttat ggaagtcgtg
    57121 aataagtatg atgtcatagt aatagaagat aatccttatg gagaaataag atttacacca
    57181 gagcctctac ctacacttaa atctcttgat actaaagata aagtattata cttaggttca
    57241 ttttcaaaag tattatgtcc agggtttaga gttgcttgga tgtgtggtaa tgtagacatc
    57301 atagataagg cagaactttt aaaacaaggt atagatttac agtcaaatca attttcgcaa
    57361 gtacaagtaa tagaattttt aaagaaatac aacttaaatg aacatataga aaaaataaga
    57421 gcagagtata agaaaagatg tctgctaatg ttagatgcta tgaagaaaca tttcccagag
    57481 gaagctaaat atacagaacc agatggagga atgtttatat gggttgaact tcctgaaagt
    57541 ataaatgtag atgatttatt ggataaagcc attgatgctg gagttgctta tgtttctgga
    57601 gagtccttct ttgcaaataa tggtccaaaa aatactatga gacttaacta tacaacaatg
    57661 tcagaagaac aaatcgttaa aggtgttgag atacttgcag aagttataaa aaaagaactc
    57721 aattatgctt gtgaataaat ataaaaagtg agggtttaaa tatgggagaa aaagaaaacg
    57781 gtataaagtt tggaaagtta atgtttcaac ttgttattat agcaatatct tgggaattag
    57841 tttatattat accatttatc caatatacat tatatgatcc aatcctaaaa gcattagaat
    57901 gtacaaacac tcaactaggt tttctactta caatttatgg gttaggtaat atatttggag
    57961 ctcctatagg aggctggtta gcagatagat ttgattatag aaagataatt ttaggttctg
    58021 tatttcttaa tggagtagta tcatttttat ttgcatttaa tatgaactat ccatttgcag
    58081 ttgctacttg ggtaggttgt gcaatcacat cattagttat gaactatcct tctatggtaa
    58141 aaatacttag agttatagga aaagataatc aaggtaaagt ttatggattc aatgaagcta
    58201 tggttggagt ttctggcgta gtaatgggag caatattctt atatatttat acatgctttt
    58261 caactcctgt attgggtatg agatgggtaa tgatatcttt aggaatatta tcaatagtga
    58321 tgtgtccagt attatggttt gtaattaaag atgtagatat cagagaagac aaagaagaag
    58381 aagcaagtga aaaaatgtct gctggcgatt ttatgacagt actaaaatca ccaaacactt
    58441 ggctagtagg aataagcata ttctgtgtat attcattcac agttacaatg tcttatttta
    58501 caccatatat aacatcagta cttggaggtt ctgtggcatt gtctggggca ttagcaataa
    58561 taagacaaca tggtctaaaa ttgtttggag caccatttgg aggatattgt gcagataaag
    58621 taaaatcacc tacaaaagta ctactaccaa tatatgtatt tggtatagca gtaataattt
    58681 tattcctagt actacctgca tctactccaa tgactatctt tatagcttta acatttgtag
    58741 taggtatatt aggttacatg ggaaaaggta tatactatgc agttcaagat gaagtaaaag
    58801 ttccagttaa atattcagca actactatag gtatagctgc agctttagga ttttcaccag
    58861 atgtattcca atttgcatta ataggacact ggatagatac ttatggcaat aaaggttaca
    58921 catatacatt tatattccag atagcaattc taataattgg tatactatct tgtttatata
    58981 tattaaaagt taagaagaaa agagaaagta agttagaagc ataaatattg taaaaatacc
    59041 aaattattaa gttttatagc aatacttaaa atttaaagtg caacacacca aaatgttatc
    59101 aagttttcta ggttttagta taaagttacc cctaaaggtc tggaattgag tagcaattat
    59161 catctattta tatatttaca taaacctatt taatttaaat taaaagaatt tcaaaaatag
    59221 gtggtaattg tactcaattt tacacctcat aataaatacg aaaatttaaa atctaactat
    59281 atactattca attatatatt aataattgta tatggtacta tttatatagt aacagtttca
    59341 ggcttttcat tacatgtatt ccaatttata ttgtttgatt atttgtcaaa tacatatcgt
    59401 taaaaagttt ataaatttac gtttatatat cgattaatat tctttgtttt agagatagat
    59461 acttatagct tgtggattaa aaataatata aatagaagat agatttttta gatatattga
    59521 gttgaagatt atatagaatg ttgaaacata gctatttaaa ttattgtcta taaatataaa
    59581 atgaatggtg gtgagttaag tgaccgattt agttaacacc attaatggaa ttgtttggtc
    59641 acctgtatta gttgcaatgt gtttgtgtat aggattatat ttttcaatga aattaagatt
    59701 ttttcaaata aaagatgtaa aagaaatgtt tactcttttg cttgaaggag atagctctca
    59761 tgttggaata tcttcgttcc aaggttttgc tacatctatg gcaggaagga taggaactgg
    59821 aaatatagct ggtgtagctg tagcgatagc aatgggaggg ccaggagcat tagtgtggat
    59881 gtgcataatg gctacattag ggtcggcaac tgcatttgta gaatctactt tagctcagat
    59941 atataaagat gagcatgatg gacagtacag gggaggtcct ccatattact ttgaaaaagg
    60001 tcttggttgg aaaaaatgtt caattttatt ttcagtggtt gcaatcataa gttatacatt
    60061 ttttatgcct ggcacacaat caaatactct atatctagca atgaatcaag catttggatt
    60121 ttcaaaaatg acaatagcag ttacatcttc tattgcacta ggtataataa tttttggagg
    60181 aattaaaaga attggtttat ttgctgagaa agtagtacct ataatgggtg gagcatacct
    60241 tattatgaca ttggtaataa tcatagcaaa tataaatcta gttccaggag ctattatgac
    60301 aatgtttaaa tctgcatttg gatttggttc ggtttttgga gggatgttag gttcagcaat
    60361 atcttgggga gtaaaaagag gtatatactc aaatgaagcg ggagaaggtt ctggaactta
    60421 tggtgcagca gcagcagaag tgagtcaccc agccaaacaa ggattagtcc aagctttttc
    60481 tgtttatgta gacacactat taatatgtat ggctacaggt attatgatta taataacagg
    60541 aatgtataat gttacagatg tttacacaac attaccaggt gtagaaccag gtccacaata
    60601 tgtacagagt gcaataaata cattacatcc tcaattaggt gatattttta tagcaatagt
    60661 tttactacta tttacattta caacattatt agccttctca tacatgatgg agaccaacgt
    60721 atcttactta gtgactagaa ttaaggcaga aaaacatgaa aagcacataa ttaatgtctg
    60781 tagatttatg cttataatat gtgcagcaat gtgttgcttt atagaaccac ttacaagttg
    60841 ggcacttggt gatatatgta taggtgtatt aacatatata aacttagttg gtatatttac
    60901 actaaaaaaa ccagctataa aagcatatta cgattatcaa aggcagaaaa agatgggagt
    60961 aaaaaggagc gatagaactt ttaatcctat agaattagat ataagaaatg ctgagttttg
    61021 ggaaaataga tacttaaaag aggataagca tgagttaaat taaagttata ataaaatata
    61081 aaaattacta aataaattta gtgggaggta agattggtat gaaagaaaga ataatacaag
    61141 tggctgattc aaagaaagaa aaaattctag ggctatgtca atctttatat gatgaaccag
    61201 aaattgcttt gcaagaatat aaatctgcta aaaaaatatc agaatttcta agagaagagg
    61261 gttttgatgt tgaggaaaac ttggcaggta tggctacagc ttttaaagca accaagaaaa
    61321 atggagatgg tcctaaaata gcatttatag cagaatatga tgctcttcca ggcaatggac
    61381 atgcatgtgg acatcattta attgcatcta tgggagttgg agctggaata gctctttcaa
    61441 gtatactgga tacttacaag ggagaagtaa gtataattgg aacacctgct gaagaaactg
    61501 gagatggaaa gccttacttg atagaacatg gtgtatttga tggctatgat gcagcaatga
    61561 tgatacatcc aaattcaaaa acttgtgtaa cacctgaaat tatagcaata ggaggtctag
    61621 atttcatatt tactggaaaa gcatctcatg caggagctaa gccatataac ggaataaatg
    61681 ctttagatgc agtagtacta ctatataata atataaatgc actaagacaa caattagtag
    61741 atggaacgag aatacatggt attatactag aagcaggaac tgctgcaaat gtaatacctg
    61801 atatgggaaa agtaagatta gagataaggg caaaagaaca aaattacttt gatgaagtag
    61861 tagaaaaagt gaaaaattgt gctagaggtg ctgcaatagc cactggatgt gaacttgaat
    61921 tttatcactt tgaaccaact tgccaaggat taaatgaaaa taaagttttg gtagacatat
    61981 ttacaaagat tatggaggaa tttggaatat atgaagatga acaagtaatg ttaggttcaa
    62041 cagatatggg aaatttaagt caaataatgc catgtataca tcctttgatg aagttttctg
    62101 aaaatggaga agaactacat acaaaagaat ttttggaggc ttctataaat tcatatgcaa
    62161 aagatagagt aattgatggt ataaaaatat tagctttaac aggatttaat ttatttgaaa
    62221 atccagagtt attgaaaaaa atgaaggaag agtagaactt agactatttt taattatact
    62281 ctttaaaata agaagagtgt ctcaaaatga actttttagt ttatgagaca ctcttttttg
    62341 catgaaatta aatatatttt tattatttta acaatgaaaa agaggcttat ctgtttttat
    62401 ataacctttt ttaacttgta gcacaaaata tcatgaatga ttacaattcg aatgataaaa
    62461 tcgattataa gatatatagg aggggtacat atggaaactt tagaagtaag aaatattgaa
    62521 ttgagtggta caaattatga gataggatac agactaggag aattggttgc taatatgcca
    62581 gagataatag aaggtcagat taataaatct aatctggtaa gcaaaaaaga agaaaaagag
    62641 atgatagagt tgtttgataa atattgtcca ggtcttaatg aagaattgca aggttttgct
    62701 gatgcaattc aagtgaattg taatcaaatt ttatattata ctatgactta tcttaaacca
    62761 ggttgtagtc aagttgcatt aacaccagaa ttaactgaaa atgggcatgt actatttgca
    62821 cgtaatttcg atttctcaca caatatggag gattttgttt tatgtaaaac taaagtaaat
    62881 ggaaaatatg cacatatagg tactaccgtt atgcaatttg gaagaggtga aggtatgaat
    62941 gaatgtggat taggtgtaag tcagtcatca tgtggtattc ctgtaggcaa ttcagatggt
    63001 ttgagaaaac cagcaattgt aggtttacag ttttgggctg ttattagata tttgttagaa
    63061 aattgcaaag atgtagatga agcattagaa tatttaaaag atatgccaat tgcatacaat
    63121 attaatttat tgcttgcaga taagtctgga aacattgcac ttgtggagac tttagatggg
    63181 aaaaaagaag taaatataat caacagttta gaaagtaaga gagaatattt tttacactct
    63241 actaatcata cacatattga taaacttcat aaattagaac cacaatcaat gaaaaattct
    63301 attcatagat ataaattaat taaagaatat ataaataagg ctaaaaaaat tggagagaaa
    63361 gagttaatga atttattgtc tttaaagtat ccaaatggct tatcttgtaa ttactataat
    63421 gatttttttg gaactttaaa aagtatagtt atggatttaa atataggaaa gtttaatatt
    63481 ttatggggtg gactagagaa taaatgggag agctactatt taaaaaatga cattaaatct
    63541 ataacacaga ggataaacat aaatattgaa aaagcaccat ctgacttttt tgatttcatt
    63601 gaataaaaaa tgtatttaat tattctaaat aattaggcta tataaatcaa ctaaaagttt
    63661 ttatttatcg tattgaatta gttataatat tttagtatag taaaaaatag tgctatttta
    63721 catatttaaa gagtttaagt agaagagagg tgccatattg gattacttaa agtattttga
    63781 aaaagctgta atgtatattg agaataattt acatgaaaat ataactgtaa atgatgtggc
    63841 aaaagaaact ggatattctt attatcattt aacacgctta tttaagtcta tgtttggtga
    63901 gagtgtagga agttatataa aaaaaagacg attagtaagt agtacaaaag aacttttata
    63961 tagtgataaa aaagtgatag atatagctat tggtagtgga tttgaatctt cggaagcatt
    64021 tagcagagca tttaaatcta tatacaaggt tagccctata gaatatagaa aaaatagaat
    64081 tgatgtattt gttggtaaaa agaaaaaatt agagttagat tttatgagac atcttgttgg
    64141 aaatataacg attaaaccag ttataaaaga aatagaaaaa ataaaggtaa taggtataaa
    64201 ggataaagta attttagaaa ataattcatt acctgacttg tgggagaaat tcagaaaagt
    64261 acatcatatt gtcccaaata ctttgccatc aaagagagtt tttggaatct gtgaagcaat
    64321 ctctgaaata cacctagtta gtgaaagtat ggaatttaat gagataatag gacttgaagt
    64381 taatagttat gatgtgattc caaattcatt tgtttcaaaa actattaagg aaggaaagta
    64441 tgctatattt actcatactg gtagcttaga taacttagac aaaacttatg aatatatatg
    64501 gggtacttgg tttttaagtt caaaagaaga actggatata agagatgatt ttgaagtata
    64561 tgatgaacga tttttaggtc ctagtaatgt gaactcacaa attgatattt atatacctat
    64621 aaaataatct ataagaaaac taaaagataa tttgattaag aaatagatgt tcgtaaaaga
    64681 gcatctattt tttattttta tcagatttag ataattttaa taataaaaga aataaaaata
    64741 agttcttaaa tttctaagaa gaatttagaa tatagtcgaa ataagaattg tatatttctg
    64801 aaaattctaa ttagtacaat tttttccttt ataaaattga aaaaagatgt atttatttat
    64861 caaaaatatt atattaaaat gatattaata aaaaaacatt tacaatatta gcatatagta
    64921 cagaaaaaag atgtaatttg taaaatttat gttaagcagt aaaattaaaa atttaaagat
    64981 attataggga ggttataatt ttatggaaaa cgttattaca ggtctaagta aaggtgcaga
    65041 gtggtttatc ggtttgtttc agaaaggggg agaacaattt ataggtcttg tttcaggtac
    65101 tcttccaacg ctaattgtat taatggtagc tattaactct ttgattaaga taattggtga
    65161 ggaaagagtt aataattggg cttctaagct aggaaagaac tcttttacaa gatatattct
    65221 acttccatta gtatcagtat ttttcttggg aaatcctatg tgctatacat ttggtagatt
    65281 tttaaaagaa gagcacaaag caggatttta tgatgcagca gtttcatttg tacatccaat
    65341 tacaggtctt tttccacatg caaatgcagg agaacttttt gtatggttgg gaatatcagc
    65401 aggtttgact actttaggta aagaaactac tacactggca ttgtggtatt ttatagttgg
    65461 tctaattgta atttttataa gaggtatagt aacagaaaaa atgtatgctt ttctaacaag
    65521 aaaaaataga gcaacaaata aggcttagga ggtagataaa atggaggaga ataaaatatt
    65581 aaaaatagaa aagggaactt caggctgggg aggtcctttg tatataaaaa aagaagggaa
    65641 tagaaataag atactatcaa tgactgctgg tggtatccat gaagtaacat taaaaataaa
    65701 agaactactg ggttgtgaaa ttgtagatgg atttaaaact ggagtttctg atgaagaagt
    65761 agcagtagtt attattgatt gtggtggaac tgctagatgt ggagtttatc caaagaaaaa
    65821 aatacctaca ataaatgtaa atccagttgg aaaaacaggt cctttagcaa aatttataac
    65881 agaagaatat tatgtatcag atgttaatcc taactgtata agtgttgtag atggggaaga
    65941 tatgccacaa aaatctcaag aaaataaaag cgaaaataag agttctatta gaaagcctga
    66001 taattatgat gaggttaagt caaaagcaca aggtgaatat gctaaaaaga acataatact
    66061 ttctattggt caaggggcag ggcaagtagt atcaaaattt tatgatgcag gtcgagacac
    66121 tatccaaatg gtaatgaata acgtaatacc atttatggca tttgtaagta tgcttatggg
    66181 aattatttta gcatctggac tgggagactg gattgcaaga gttatatctc cacttgcagg
    66241 taatattggt ggattattga ttatatcagt aatttgcaca cttccattct tatcaccaat
    66301 actaggacct ggtgcagtta tagctcaagt agtagggact cttgtaggta cacaaattgg
    66361 tcttggtgca ataccagcat atttagcact tccagcttta tttgcaataa atgggcaagc
    66421 tggatgtgat tttgtaccag taggattaag tttgggagaa gcagaaccag aaactgtaga
    66481 gtatggtgtt ccagctttat tttactctcg tttaataact ggtccaatag ctgttattat
    66541 tgcatatgga gttgcagtat ttgcattgag ataatattta atgtcaatat aaggcttgga
    66601 attaattaat aaaattgtaa atataaattt aagctagtca agattttttg ttaatgaaag
    66661 actagctttt atactattaa ttagctgtaa ttatcgagtt agattaatac taaattttcc
    66721 tttattaaag tgaaatttta tgtattaatt tggtttcaaa tttgtactag aatgaaatca
    66781 aggaggagat acaaaatgag tgtaactgag aaaattcctt ctattgaccg caagatattt
    66841 aagatattga ttatgtgtag taaaaaagag tttgtgagta ttaattcaat agcaaatgaa
    66901 ttgaatgtca caactagaag tgtaaggacg tatataaaac aattgaataa agatttaggt
    66961 aatgatatag cagtaatcaa atatataaag ggtcaaggct ataaactaga aataaaagat
    67021 gagcagatac tcaataagat tattgatatg aatagaaaaa atgtattttc attaaattca
    67081 aaagaagata gagtagagtt tatactaaat tacttgatag aactagatgg atttattaca
    67141 cttgatagtt tagctgatga gatgtgtgta ggaagaacta ctttagtaaa tgatttccag
    67201 tatgtagaaa aagttttggc ttcatataat ttaaacttga ttaaaaaaca gaatacaggt
    67261 atgaaattaa atggaaatga acttgatatt agactgttta tattaaatca gttgtataaa
    67321 aactcaagga aagatttcag taattcaaag tattttaaag gaatcaaaaa agaagaaatc
    67381 ataaatttag aagaaaaact attgacttta tttaaagaaa ataattttta tgttacagat
    67441 gaaatgttaa gagaagttat taactacata gtagttttag tctatagagt aaaagaagaa
    67501 aagaaagtaa aagactatga tgtaaagttt gatttactta aatcatatga tgaatacttt
    67561 attgctaggg aaataaaaca tataatatca ggaatgtttg aatgtacttt aaatgatgaa
    67621 gaaattattt atttaactat accattagtt agtggaaatg caccagcatc agaatgtgct
    67681 ttaaatagta gtagaattag taaaaacatt gatgagttga tggaaaatat atttaatcaa
    67741 atatatgtcg atatgggtat ttttataaat gaggatgaat taagagtagg tctaggatat
    67801 catcttagtt ttactttaaa caggctatta tttaatataa agttaaaaaa tgttttgtta
    67861 gaagagatta agcaaaatta tatacttcca tttaaattag cacaaatagc aggaaaagta
    67921 atagagaaaa agtataactt agaagtttca gaagatgaga ttggatatat agcaatccat
    67981 ttcagtggat atttagaaag aaatagcagt agattctatt caataaaaaa aatagcaatt
    68041 atatgtagta caggtctagg aacagcaaaa ctattgaaaa taagagtaga aaaactgata
    68101 ggaaataatc ctaaaattga tactctttca agttttaatt tgagaaatat taacttagat
    68161 gaatacgata tagtttttac aactattgat ttagacacat cgaatataaa tacaatagta
    68221 ttaaaaataa atacaatatt tgatgaaaac aaattaagag aacaattaaa aacagtattg
    68281 tgcctaagag aagggaatat tgacactact aattctacaa atttactaat aaataatttg
    68341 ttagatgaag ataaatttat gatattaaat gaaaagacta tacttgcttc attagaaaaa
    68401 atgatggacc atcttatgga tttgggatgt attgatgata agtttagaaa aaatatagtc
    68461 aatagagaag aaaaatctcc cacagtattt gataaaggtt tattatttcc tcactctgta
    68521 aatgaaaaat cagataagtt tttaatggca gttggaattt tagaagaacc aatagaatat
    68581 gcaaatagaa gtataaaaat aattcttatg acaatgtttc catgtgagaa taagatagac
    68641 tcagacttat tagtaaaaat ttatgaagaa gttttaaaga taggccaaga tgtaaaatta
    68701 acgaataaga taagtaaatg tagaactttt ttagaattta aaaaggtgtt acttaaaaac
    68761 cttatataac tattgtacag catttatagt tgttatacag atttaaaaat aatgtgattt
    68821 ttggaaggtg tataaatatg aaattattag taattttatt tgttgcttat attttaaata
    68881 ctatatttat aatgaagcag aataaattgt attttaaaac tttaaataga gctaaaaaaa
    68941 agggtgatat agtgtctaca ggcaaaaaga aaagctattt ttctaaagga agtatagcca
    69001 ttatatcttc agattcagat gggtttataa agtttggaga aattttaaaa gggcgtacag
    69061 taatggctaa atttaaagag attgaagaaa tagaaggatt agatatatat agtgcagagc
    69121 aaaaatttaa agatgaagag agtattgtac aagctataaa atttataaaa gaaaaaataa
    69181 atgtttcttt taattaactc aatataaata taaattatca ggaggaatgt catgaaatat
    69241 gaagttaaga taagtgggat aggcagttta gttaaagagt taatggatga aagcaattgt
    69301 cttattatat atgatgaaac cattaatgat gacgacttaa aggatatatc tgtaattcat
    69361 tcgatagcaa cattaaaaag tgatgttgaa ataggagata cacttactat agggaataga
    69421 gattattgta tagtttcagt tggtgatatt gctcagaaga ctcttagaga aattgggcat
    69481 tgtacaatca aatttgatgg aaaatgtgaa gtaaatttac caggagaaat acatgtggaa
    69541 ataggaaatc cagatataac tattggagat ttaataacta tttcttaatt tgaaaataaa
    69601 attattataa caggaggaat tggtatgtta gatttaaaca gaaaacttgc aaaattggaa
    69661 gaggaaggta aaaaaataaa agtagctctt ataggagctg ggcatatggg aaatggaatg
    69721 gtaagccaaa tggcaaatat gaaaggtatt gaagcttcta ttgttgtaga tataaatttg
    69781 gagttggctc ataaagcttt tactgatgca ggaatagatg aagaaattat agataatgta
    69841 acaaatgaaa aggatgcaga aataaaatta caagaaggaa aagtattgac atgtaatgac
    69901 tttcttgtgg cttgtaaaac taagtcaata gatgtagtta tagatgcaac tgggggtatt
    69961 gcaattggtg cagagatagc attaaactct attttaaata aaaaacatat agttatgtta
    70021 aatgctgaaa ctgattgtgt tgttggacca atacttaaaa aattagcaga tgatgctgga
    70081 gtgatattta caggttctgc aggagatgag ccaggtgcag taatggaatt atttgatttt
    70141 gcagatgcaa tggggtttga agttagagtg ataggtaaag gtaaaaataa taaattagat
    70201 ttagattgta atcctgatac agttagagaa gaagcagaaa gaaaaggagc ttcacctcat
    70261 atgatagctt cttttaaaga aggaactaaa acaatggtag aaatggcttt aatgtgtaat
    70321 gcaactggat ttgtgcctga tgtaagaggt ggtcatggta ttgaagctac agttaatgaa
    70381 gtacctaaga aatatgcatt aaaaagcgaa ggtggagtat tagataatta tggtgtagtt
    70441 gattttgtaa atggtatagc acctggagtt ttcgtagtag tagcccataa attaaaagca
    70501 gttaatgatg aactaaaata tttaagtatg ggtgatggac ctaattacat attatataga
    70561 ccatatcatt tatgtagttt agagactcct ttatcagcag ctatggcagt cttagaccat
    70621 aaggctacaa tagtacctaa agctggacta gtagcagaag ttatgactat agctaagaaa
    70681 gatttgaaaa aaggtgagtt tatggatggt tatggagcat atacatgcta tggtactata
    70741 gaaaaatacg atgtagcaaa ggctatgaat gctgtaccta ttggtttgat aagtaaaaag
    70801 actaaagttg taaaagatat taaaaaaggt gaagttataa cttacgatat ggtagagata
    70861 gaaaaagata ctactttata tcatttaaga caattgcaag aaaaaatatt tgggtaagat
    70921 ttgatggtaa tataaataga tattagtaaa aagggttgat ggttattttt aaatcaaccc
    70981 ttttaaattt ttatatattc ttgttaggat actttcttta aaagtatatt ataggttatt
    71041 tgtagtcgta aaaattattt tgaattagtt ctgcaatttt atattcacat tgaaacaata
    71101 ttattaaaaa ttttaatata aaatgaatta aaatgaccaa ttatgtatta taatattaac
    71161 tgtaatacta atatagttct attaattaaa gaaatatccc ccattaatat ataataaata
    71221 tctttaagcg agagaactta gtaaaaaata tatcgtattc aataaaaaat ctgtttacag
    71281 aatggtatat atagttatat aatatactat ataagatgcg aaaagataaa aagtatagca
    71341 caatgaaaga aggtgattat tattcagctt aatgaaagac aactaaaaat tatagatata
    71401 gttaaggaaa atgaaccaat aacgagtgaa agtattgcct ccagcttgaa tgtgactcgt
    71461 gctacactta gatctgattt agctatatta acaatgacag gaatattaga cgcaagacca
    71521 aaagttggat atttttattc aggagttagt gaaataaatt taattggtaa aagtgtaaaa
    71581 gaaaaaacag tagaggatat aatgagcatg cctgtactag ctaagaagga tgaaagtata
    71641 tatgatgtaa tagtcactat gtttttatct gatgtaggga gtatagttat tatagatgag
    71701 aatgaagagt tatgtggagt tgtttccaga aaagacttgc taaaagcaac tataggaggt
    71761 tctgatataa ataaaatgcc aataggtatg ataatgacaa gaactccaaa tgtagtcact
    71821 ttaactaaag gagcaagtgt tttattagct tcaagaaaaa taatagaaca tgaagtagat
    71881 tcgattccga ttgttgaata taagggagaa gacaaaaatc atatgagagt tgttgggaga
    71941 atatcaaaaa ccaacataac taagctattc ttagaaatag ttgacaatta aggaggtatg
    72001 ctggtgaaaa acctaattat atatgcagta tcagattcag taggggagac tgcacaacaa
    72061 gttgcaaagg catgtatgtc tcagttttat gtgaatgaaa cttatgaaat aaaaagattt
    72121 ccatacatga ttaataaagg agtactactt gaaactttgg agaatgcaaa agctgaaaac
    72181 gctttaattg tatatacatt agttgatgaa gaattatgca gtattgttga aagatattgc
    72241 gaaagagaag gtcttagttg tatagattta atgacagata tactaagaga aataagcaag
    72301 agaacaggaa gaaaacctaa aagagaagct ggtataatta gaaaattaga tgaatcttac
    72361 tttaagagag ttgaagctat agaatttgca gtgaaatatg atgatggaaa agaccctaga
    72421 ggtgttcttc aagcagacat aattttagtg ggaatatcaa gaacatctaa aactcctttg
    72481 agtatgtatt tagctaataa aaatataaaa gtagctaatg ttccattagt tccagaaata
    72541 ccaattccta aagaagtgtt tgagatagat actaaaaaga taataggtct tactaattca
    72601 cctgaaaagc tgaatgagat aagaacccaa agattaaaag ctttaggatt gtccagtaaa
    72661 gcaaactacg ctaatttaga aagaatactt caggagttag attattcaga agagataatg
    72721 aaaagaattg gatgcccagt aataaacgtt tcaaataagg caatagaaga aacagcaggt
    72781 ataattttag acataatgaa agaaaatggt ctaaagatat acaaagaaat agaaatttaa
    72841 taatgtctat taataaaaat gatataaatt aaaataaaaa gattaatggg ggatttaaca
    72901 atggaaacta agtatgttta tagttttggt gaaggaagca aagacatgaa atctttacta
    72961 ggaggtaaag gtgccaattt agcagaaatg actaagatag gcttacctgt ccctcctgga
    73021 ttcactataa caacagaagc atgtaatgat tattatgtta acaatgaaag tataagagca
    73081 gagataataa aagaaataga agagcattta gctactttag aaaaagactt aaataaaaca
    73141 ttaggatgca ataagaatcc attattagta tcagttcgtt ctggtgcagt attctctatg
    73201 ccaggaatga tggatacaat tcttaaccta ggtcttaatg acaatagtgt tgttggctta
    73261 gcagaggcta cacaaaatga aagatttgca tatgatagtt acagaagatt catacaaatg
    73321 ttttctgatg ttgctatgga agttccaaaa tataaatttg aaaatgtatt agacagagta
    73381 aaagaagcaa agggatacac agttgataca gaacttacaa ctgatgattt aaaagaaata
    73441 gtaaaagaat ttaaagcaat ttataaaaaa gaaataaaaa atgatttccc acaagaccca
    73501 aaagagcaat taatgcttgc aatagaggct gtatttagat catggaataa tcctcgtgca
    73561 atcgtatacc gtaaattgaa tgatatagct cataacttag gaacagcagt aaatattcaa
    73621 tcaatggtat ttggtaatat gggagaaact agtggtacag gtgttgcatt tactagaaat
    73681 ccagctacag gagaaaataa attatttggt gaatttttaa tgaatgcaca aggtgaagac
    73741 gttgttgcag gtataagaac tcctcaaaat atatcaactc ttgcagatgt aatgccagct
    73801 gtattcgatg aatttgtaaa aattactcat atacttgaag gtcattataa agatatgcaa
    73861 gatatagagt ttactataga aaatgaaaga ttatatatac tacaaactag aaatggtaag
    73921 agaacagcag cagcagcaat aaatgtagcg gttgatttag ttgaagctgg aataattgat
    73981 gaaaaagaag ctatcatgag aattgagcct aatcaattag accaattatt acatccaaaa
    74041 tttgaagata aggcattaaa agaagccaag gttatagcaa aaggtttacc agcatcacca
    74101 ggtgcagcaa gtggtaaagt ttatttcaat gctgatgatg tagtaaaagc aaatgaaaaa
    74161 ggagaaaaag tagttttagt tagattagag acatctccag aagatataga aggaatggtt
    74221 aaagcagaag gaatccttac agctagaggt ggaatgacat cacatgcagc agttgttgct
    74281 agaggtatgg gtaaatgttg tgttgctgga tgtggagaaa taaaagttga tgagtttaat
    74341 aaagaaataa gagcacttga tgatgtagtt ataaaagaag gtgaatacat ttctatagat
    74401 ggttctacag gaaatgtgta cctaggagat gttaagaaaa cagaagtatc tttaactgga
    74461 aactttgaaa aattaatgaa ctgggtagac aaacacaaat gtatgatggt tagaactaat
    74521 gcagataatc ctagagatgc tcgtgcagct atagagtttg gtgcagaagg tataggtcta
    74581 tgtagaactg agcatatgtt ctttgatgaa gctagattgc cagcagttag agaaatgata
    74641 ttatctaata cagtagaaca aagagaaaaa gctcttgaaa aaatattacc aatgcaaaga
    74701 gaagattttg tagagttatt taaagttatg gatggaaaac cagttaatat aagattacta
    74761 gacccaccac tacatgaatt tttgcctcat gatgatgaga ctatagaaga actttcaaaa
    74821 tctatgggaa taaaagtaag tgatattaag aagagaatag ttgatttaga tgagtttaac
    74881 ccaatgcttg gacacagagg ttgtagactt gcaataactt atcctgaaat atgtgtaatg
    74941 caatctaaag ctataataca aggtgctata gaagcaatca aagcaggtgt taaagtttct
    75001 ccagagataa tggttccatt agttggagaa gtaaatgagt taaaaataat aagaaaaatg
    75061 atagtagaaa ctgttgacgc tataataaaa gaagaaggta tagaagttcc ttatactgta
    75121 ggtactatga tagaaatacc tagagcttgt ttaactgctg atgaaatagc acaagaagca
    75181 gacttcttca gttttggtac taatgactta actcaaatgg catttggata ttcaagagat
    75241 gatgctggta aattcttagg tcaatatgta gatgaagaaa tattagaaaa agacccattc
    75301 caagtactag accaaaatgg tgttggtaag ttagttaaga tgggagctaa attaggtaga
    75361 gaagttaaac cagaattaaa actaggaata tgtggagagc atggaggaga accatcttca
    75421 gtagagttct gttatagtgt aggacttaac tatgtatctt gttcaccatt tagagtgcca
    75481 attgctagac ttgcagcagc tcaagcatct ataaaaaatc ctagataata atagactgca
    75541 aaatatgtaa ctaaataaaa ataaaatccc ctattgttca atgtatcaaa ttaagtattt
    75601 gtaaattgaa tataggggat ttttattata aaaatttaac ttatacagtt gtaagtgaaa
    75661 cgtatttaat tatattgcat atattattgt agagagaaat ttcaactatc aattaaattg
    75721 agtttactgg aggggataaa gttgacaaag tgtcctaaat gtggaaagga agtatcatca
    75781 cgtccaggta gtatatgccc acgatgtgga tttaaagtag cagatgagag tctaaaaata
    75841 agatgtccag aacctagttg tagggctttg gtatctagga aactggatta ttgtcctaaa
    75901 tgtggatgca aacttagagg atataatatt tctgaatttg ttaacatggt aagatgtaaa
    75961 atagatgaga catttgataa aaaataatta aatttagagt taagcttttg agggtgtctc
    76021 aaaatgaact ttttagttca gaaggcactc tttttgtatg gaatcaaata tatttttata
    76081 atttaaacaa taaaaaagag gcttatctct attttagtat aatcccctta tagtagacat
    76141 ttaaataaaa ggctattata ataagtctaa aaagcgtata ctctaagggg gtttttctat
    76201 gtctaaaaaa cataaaaaat atagcaaaga acttaaacta aaagctgtaa atttatatat
    76261 aaaagaagga tactcatcat acaaaattgc tgagatgctg aatataagaa gcaaaacaca
    76321 agttcaaaac tgggtaaaag attataaaaa taaaggtaaa actgcattta atgaggaaac
    76381 tagaggcaga tttaaaaata ttagtctaga aaatgataat agaaaattta aatctgttga
    76441 agaagaattg aagtacttac gcatggagaa tgaattttta aaaaagttaa gtaccctatt
    76501 ggacaagtca aagtaagcga taaatttaaa acaattaaac ttatgtctag gaagtatagt
    76561 atttctagta tgtgtaagtt gataggtgtg tcaagaagcg gttactataa atggctaagt
    76621 tattctaaaa aatcttccga taggggtatc aaagatagaa ttattaagga ttatataatt
    76681 gaaatacata aaaaatatag aggaacttac ggtagaaaaa gaatttgtac ttatttaaat
    76741 aaaatacttg atagccctat taatcataag aaagtatata ggttgatgaa agaactagga
    76801 attaaatcta ttattagaaa gaaagtatat agacgtaagt ttaaatcata tgaggtatat
    76861 gataatatat taaatcgtga atttagagct aatcaaccat tagaaaaaat ttgtat
//
LOCUS       DAELXX010000005        67497 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.005, whole genome shotgun sequence.
ACCESSION   DAELXX010000005 DAELXX010000000
VERSION     DAELXX010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 67497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67497
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.005"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(121..894)
                     /locus_tag="KPH29_000296"
     CDS             complement(121..894)
                     /locus_tag="KPH29_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HBG1465941.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
     gene            complement(894..1280)
                     /locus_tag="KPH29_000297"
     CDS             complement(894..1280)
                     /locus_tag="KPH29_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG1465942.1"
                     /translation="MEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVAVSEACTN
                     AIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLGLFIIKTL
                     MDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            complement(1305..1622)
                     /locus_tag="KPH29_000298"
     CDS             complement(1305..1622)
                     /locus_tag="KPH29_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HBG1465943.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVKKIFTITGLDKIFK
                     VEG"
     gene            complement(1634..1894)
                     /locus_tag="KPH29_000299"
     CDS             complement(1634..1894)
                     /locus_tag="KPH29_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBG1465944.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            complement(1894..2190)
                     /locus_tag="KPH29_000300"
     CDS             complement(1894..2190)
                     /locus_tag="KPH29_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG1465945.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="KPH29_000301"
     CDS             complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="KPH29_000301"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBG1465946.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDIAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKIGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKNEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="KPH29_000302"
     CDS             complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="KPH29_000302"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBG1465947.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKIYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            complement(6607..7722)
                     /gene="recF"
                     /locus_tag="KPH29_000303"
     CDS             complement(6607..7722)
                     /gene="recF"
                     /locus_tag="KPH29_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBG1465948.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            complement(7740..7946)
                     /gene="yaaA"
                     /locus_tag="KPH29_000304"
     CDS             complement(7740..7946)
                     /gene="yaaA"
                     /locus_tag="KPH29_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBG1465949.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVKIEGTKIKVL"
     gene            complement(8083..9189)
                     /locus_tag="KPH29_000305"
     CDS             complement(8083..9189)
                     /locus_tag="KPH29_000305"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBG1465950.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            complement(9431..10753)
                     /gene="dnaA"
                     /locus_tag="KPH29_000306"
     CDS             complement(9431..10753)
                     /gene="dnaA"
                     /locus_tag="KPH29_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBG1465951.1"
                     /translation="MMDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILL
                     APSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYP
                     NLNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHH
                     IVSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERT
                     QEEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIA
                     ILRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEA
                     LKDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTD
                     LSLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            11409..11546
                     /gene="rpmH"
                     /locus_tag="KPH29_000307"
     CDS             11409..11546
                     /gene="rpmH"
                     /locus_tag="KPH29_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBG1465952.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            11624..11968
                     /gene="rnpA"
                     /locus_tag="KPH29_000308"
     CDS             11624..11968
                     /gene="rnpA"
                     /locus_tag="KPH29_000308"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBG1465953.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            12000..12251
                     /gene="yidD"
                     /locus_tag="KPH29_000309"
     CDS             12000..12251
                     /gene="yidD"
                     /locus_tag="KPH29_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBG1465954.1"
                     /translation="MNKIIYILKEISRYLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRLLKCHPFHPGGYDPLK"
     gene            12283..12990
                     /locus_tag="KPH29_000310"
     CDS             12283..12990
                     /locus_tag="KPH29_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBG1465955.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            12959..13588
                     /locus_tag="KPH29_000311"
     CDS             12959..13588
                     /locus_tag="KPH29_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HBG1465956.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            13700..15079
                     /gene="mnmE"
                     /locus_tag="KPH29_000312"
     CDS             13700..15079
                     /gene="mnmE"
                     /locus_tag="KPH29_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBG1465957.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGERALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNILDNENIIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKEIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            15174..17069
                     /gene="mnmG"
                     /locus_tag="KPH29_000313"
     CDS             15174..17069
                     /gene="mnmG"
                     /locus_tag="KPH29_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBG1465958.1"
                     /translation="MIKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSI
                     ALMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQAD
                     KFKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVY
                     LNSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMS
                     LQEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPR
                     YCPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLEN
                     CKIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINA
                     VRKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLT
                     QKGYDIGLVKKDRYERFLNKKAAVDKEFERLKNERVTPKEVNSLLEEKGATPIKVGIS
                     LYEFLKRPEVTYELLEELGKGAGEDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLE
                     NKKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQVRR
                     TRGGKGE"
     gene            17062..17781
                     /gene="rsmG"
                     /locus_tag="KPH29_000314"
     CDS             17062..17781
                     /gene="rsmG"
                     /locus_tag="KPH29_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBG1465959.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVEWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKNEDYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGRPAKNPIK"
     gene            17956..18741
                     /gene="noc"
                     /locus_tag="KPH29_000315"
     CDS             17956..18741
                     /gene="noc"
                     /locus_tag="KPH29_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HBG1465960.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            18876..19649
                     /locus_tag="KPH29_000316"
     CDS             18876..19649
                     /locus_tag="KPH29_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBG1465961.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            19651..20514
                     /locus_tag="KPH29_000317"
     CDS             19651..20514
                     /locus_tag="KPH29_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBG1465962.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            20547..21689
                     /locus_tag="KPH29_000318"
     CDS             20547..21689
                     /locus_tag="KPH29_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBG1465963.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKSNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVSDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(21823..22398)
                     /locus_tag="KPH29_000319"
     CDS             complement(21823..22398)
                     /locus_tag="KPH29_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HBG1465964.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDTSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKPTPKEETKTQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            22658..23950
                     /locus_tag="KPH29_000320"
     CDS             22658..23950
                     /locus_tag="KPH29_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG1465965.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSENKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            24055..24657
                     /gene="yedF"
                     /locus_tag="KPH29_000321"
     CDS             24055..24657
                     /gene="yedF"
                     /locus_tag="KPH29_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBG1465966.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            24730..24993
                     /locus_tag="KPH29_000322"
     CDS             24730..24993
                     /locus_tag="KPH29_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HBG1465967.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            25008..25193
                     /locus_tag="KPH29_000323"
     CDS             25008..25193
                     /locus_tag="KPH29_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBG1465968.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            25296..26492
                     /locus_tag="KPH29_000324"
     CDS             25296..26492
                     /locus_tag="KPH29_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG1465969.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLEKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            26706..26984
                     /locus_tag="KPH29_000325"
     CDS             26706..26984
                     /locus_tag="KPH29_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBG1465970.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            27021..27455
                     /gene="ssb"
                     /locus_tag="KPH29_000326"
     CDS             27021..27455
                     /gene="ssb"
                     /locus_tag="KPH29_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG1465971.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            27474..27701
                     /locus_tag="KPH29_000327"
     CDS             27474..27701
                     /locus_tag="KPH29_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBG1465972.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            27844..28164
                     /locus_tag="KPH29_000328"
     CDS             27844..28164
                     /locus_tag="KPH29_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBG1465973.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            28172..29128
                     /locus_tag="KPH29_000329"
     CDS             28172..29128
                     /locus_tag="KPH29_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HBG1465974.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            29139..31133
                     /locus_tag="KPH29_000330"
     CDS             29139..31133
                     /locus_tag="KPH29_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBG1465975.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            31134..31583
                     /locus_tag="KPH29_000331"
     CDS             31134..31583
                     /locus_tag="KPH29_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBG1465976.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            31602..32930
                     /gene="dnaB"
                     /locus_tag="KPH29_000332"
     CDS             31602..32930
                     /gene="dnaB"
                     /locus_tag="KPH29_000332"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBG1465977.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            33135..33443
                     /locus_tag="KPH29_000333"
     CDS             33135..33443
                     /locus_tag="KPH29_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1465978.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            33525..34814
                     /locus_tag="KPH29_000334"
     CDS             33525..34814
                     /locus_tag="KPH29_000334"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBG1465979.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNKIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            35140..35215
                     /locus_tag="KPH29_000335"
     tRNA            35140..35215
                     /locus_tag="KPH29_000335"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35173..35175,aa:Lys,seq:ttt)
     gene            35222..35296
                     /locus_tag="KPH29_000336"
     tRNA            35222..35296
                     /locus_tag="KPH29_000336"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35255..35257,aa:Glu,seq:ttc)
     gene            35301..35376
                     /locus_tag="KPH29_000337"
     tRNA            35301..35376
                     /locus_tag="KPH29_000337"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35334..35336,aa:Val,seq:tac)
     gene            35410..35484
                     /locus_tag="KPH29_000338"
     tRNA            35410..35484
                     /locus_tag="KPH29_000338"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35442..35444,aa:Thr,seq:tgt)
     gene            complement(35867..36859)
                     /locus_tag="KPH29_000339"
     CDS             complement(35867..36859)
                     /locus_tag="KPH29_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG1465980.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKEKLLSESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(36868..37926)
                     /locus_tag="KPH29_000340"
     CDS             complement(36868..37926)
                     /locus_tag="KPH29_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBG1465981.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNGEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            38051..39514
                     /locus_tag="KPH29_000341"
     CDS             38051..39514
                     /locus_tag="KPH29_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HBG1465982.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNIDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSIGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFDVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            39616..40413
                     /locus_tag="KPH29_000342"
     CDS             39616..40413
                     /locus_tag="KPH29_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG1465983.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            40426..42528
                     /locus_tag="KPH29_000343"
     CDS             40426..42528
                     /locus_tag="KPH29_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC/EAL
                     domain-containing protein"
                     /protein_id="HBG1465984.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVIMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTITSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEKEI"
     gene            42596..43075
                     /gene="rlmH"
                     /locus_tag="KPH29_000344"
     CDS             42596..43075
                     /gene="rlmH"
                     /locus_tag="KPH29_000344"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBG1465985.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKDKEGKKILGKIKHNSYVIVLAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSKMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            43224..43535
                     /locus_tag="KPH29_000345"
     CDS             43224..43535
                     /locus_tag="KPH29_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG1465986.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            43583..43900
                     /locus_tag="KPH29_000346"
     CDS             43583..43900
                     /locus_tag="KPH29_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG1465987.1"
                     /translation="MDEKIIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SKEVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
     gene            44113..44814
                     /locus_tag="KPH29_000347"
     CDS             44113..44814
                     /locus_tag="KPH29_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG1465988.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKKTIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
     gene            45038..46339
                     /locus_tag="KPH29_000348"
     CDS             45038..46339
                     /locus_tag="KPH29_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG1465989.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKATLDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            46476..47570
                     /locus_tag="KPH29_000349"
     CDS             46476..47570
                     /locus_tag="KPH29_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBG1465990.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMVSKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNEYQVVFNVVAKEKTSEVEKIIMVNEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNKNIFKRGDVVIGNDDFGKYKGELQLILQDCTDERK
                     NKVGRIKEDEIILLDFIEPWSKFKIILEGE"
     gene            47572..48705
                     /locus_tag="KPH29_000350"
     CDS             47572..48705
                     /locus_tag="KPH29_000350"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HBG1465991.1"
                     /translation="MKIDILIKCGKTVDKSIIDIAILDDKIIEVKNHIDDSKYQAKQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGEFY
                     EDTKKYKTNVYALINIAKQGITSQDELSNMMNIDEYELKNAVRKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEAKERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIIDKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGNSIEENE"
     gene            48723..49832
                     /locus_tag="KPH29_000351"
     CDS             48723..49832
                     /locus_tag="KPH29_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HBG1465992.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFL
                     IIEELVNKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDAVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEINFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDNIYNRIKNIVES"
     gene            49897..50637
                     /locus_tag="KPH29_000352"
     CDS             49897..50637
                     /locus_tag="KPH29_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HBG1465993.1"
                     /translation="MNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGHVLVGVLSKNY
                     STVEAAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMGGSSIK
                     FFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGDTKVEDIGVLYKIMKELLG"
     gene            50642..51634
                     /locus_tag="KPH29_000353"
     CDS             50642..51634
                     /locus_tag="KPH29_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBG1465994.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYIITGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEEMDIVHICGIAMQLTDSVKKIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIDIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
     gene            complement(51821..52702)
                     /locus_tag="KPH29_000354"
     CDS             complement(51821..52702)
                     /locus_tag="KPH29_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG1465995.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            52975..54363
                     /locus_tag="KPH29_000355"
     CDS             52975..54363
                     /locus_tag="KPH29_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1465996.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPNNPVEIGKWWRPDQYADGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFMPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKAGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            54391..55344
                     /locus_tag="KPH29_000356"
     CDS             54391..55344
                     /locus_tag="KPH29_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBG1465997.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEAIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLESGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIEPLTKEKQAGVSLG"
     gene            55404..55628
                     /locus_tag="KPH29_000357"
     CDS             55404..55628
                     /locus_tag="KPH29_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1465998.1"
                     /translation="MNEKPSFKQIEIILAVIFLVLTYYKIGLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIRQAREATNYNIGNG"
     gene            55621..56133
                     /locus_tag="KPH29_000358"
     CDS             55621..56133
                     /locus_tag="KPH29_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBG1465999.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQKDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTIMTGENIINLQDICIQIAVAMAVGVWYVGAGVDLFTIY
                     ILSKDKKKGN"
     gene            56165..57019
                     /locus_tag="KPH29_000359"
     CDS             56165..57019
                     /locus_tag="KPH29_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HBG1466000.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENAESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            57586..59673
                     /locus_tag="KPH29_000360"
     CDS             57586..59673
                     /locus_tag="KPH29_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG1466001.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLF
                     INMEDISINHFINSIKVSRSTVNLDFKDLIPELEKKNIKVKNNRINGYYIVGNEMEIR
                     RVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLIEF
                     IYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPYNIM
                     YISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFR
                     PAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHF
                     ATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENIS
                     EPVDIVFTTNYNAEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIYLRQPKIDEV
                     MDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNW
                     EEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAI
                     GIATLDSPIEFGNNENDPVKYVFCLSAIDNNSHLRAMSELVELLEEDEFFKVLDSAKE
                     AKEIIDFIKTHEL"
     gene            59688..59975
                     /locus_tag="KPH29_000361"
     CDS             59688..59975
                     /locus_tag="KPH29_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG1466002.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            60002..61273
                     /locus_tag="KPH29_000362"
     CDS             60002..61273
                     /locus_tag="KPH29_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HBG1466003.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            61331..61930
                     /locus_tag="KPH29_000363"
     CDS             61331..61930
                     /locus_tag="KPH29_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG1466004.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIVLQSLSVVLQYAKGLNTQQYLKWHPAGSLGKSIREGI"
     gene            61935..62840
                     /gene="dapA"
                     /locus_tag="KPH29_000364"
     CDS             61935..62840
                     /gene="dapA"
                     /locus_tag="KPH29_000364"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBG1466005.1"
                     /translation="MKYKGIICAMITPFDEKQNINSQATCKLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVIDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPGKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEVTQNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKAQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            62946..63578
                     /locus_tag="KPH29_000365"
     CDS             62946..63578
                     /locus_tag="KPH29_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HBG1466006.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGEILCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGINLENLHIWLENGVDVCGFGGLLTKGTIEDITENAKKIREIIKNIREI"
     gene            63993..66164
                     /locus_tag="KPH29_000366"
     CDS             63993..66164
                     /locus_tag="KPH29_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBG1466007.1"
                     /translation="MKKIIYVLFTIELLIVSLLGLNIVKDNEINNILYNDTTSISVMF
                     KDYKKLNKNYSKWIKDIADENNVAISKYVFNNNEKLSIYTTDTSLNNNIKLKSGKFPN
                     TNSNEFISNINTNNSNNSNQVGKFSTMSKDISILIRDFGKISEVGESGILYISTQNED
                     TINKILRELNVDRSIFVARLHDRYVNSNLYLNPSIIRDLILIILCFLATIIHYSISVS
                     REASILRLNGYSKLDIIARVIKNIFRIMILSSVTALLIYIIYILKLNIGVRACLYFAM
                     FSIICILINILISILIVGFNSRSSKYVLSLNGKKSYGFVNIMHFTLKIVFVSFLILSI
                     NNCILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVIKNAKIKGFYKNLV
                     EEKDAFLIDSTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIKINKNYLKVNPIYSNGK
                     EIFNLIDYDDKTINLLVPKKLQVHENEIKKEFRELFYFEKIEVENMYNKELNRELNKT
                     KKSDLNVNIIYVDNNQSYFTYNSLVMDDNRNLIDDPIAIIDIDNIDDSFYLSYISRCV
                     YFNSKKLDALADISNIIEAQGVEAHIQSLHAVYNEYGLEINKLEKYLNSEIFTIIIIA
                     ISNLMITYNIVASYYERNKYKLYIKKIFGYSTTARSMLLIVSLILTNIIPIGIISTRV
                     DLSNNIILFGLLILVIEVIVSIVLDKIISNSSFNKIIKGEH"
     gene            66169..66786
                     /locus_tag="KPH29_000367"
     CDS             66169..66786
                     /locus_tag="KPH29_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1466008.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNEKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKNLNKNGKTIVFVTHDLELSEQADRVIKL"
     gene            66975..67319
                     /locus_tag="KPH29_000368"
     CDS             66975..67319
                     /locus_tag="KPH29_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBG1466009.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
BASE COUNT        25650 a         8136 c        11248 g        22463 t
ORIGIN      
        1 atataaatta acctactgtt taattttcaa tgttcgtgtt tatttcttgt tccttttttc
       61 aaggacaagt aatactatac catctttaaa acagtaggtc aatacttttt ttatattttt
      121 ttataaattt ttttcatatt cttttttcag cttctctact acctttttct ctagtctaga
      181 aacagtcatc tgtgatatcc ctagttcttt agctatacta gattgtgttt tatcaaagaa
      241 aaatctatct ttaaaaattt taacttctaa ttcatttaaa gtttcaataa acttattaat
      301 aaaatcttca tactctatac ttccaaagtt accttcttct tttccaatct tatcaataag
      361 agttatatct ttatcctctg aatcatcatt acttgatgaa tccaaagaca ttggttgata
      421 tccataagaa gcctccatag cctctaaaac atcttcttca ctaacgccta tataatcagc
      481 tatatctttt acctttggat gcttcttatt ttcttgttct aatttaatct tcgcatcact
      541 tatctttttg cttaattcct gtattcttct tgggactcta agtgtccata ccttatctct
      601 aaaatacttt tttatttctc ctactatagt tggtgtcgca aagctggaga actcaaatcc
      661 tttctcaaca tcatatctat caatagcata aattaatgcc aatgaagcaa cttgatatat
      721 atcttcaaaa tcaacacctt tgtttatata cttcttagct aacaatcttg ctaaatacag
      781 atgtctttca ataagaatat ttctaatgtc tacattttta tttttattat ataaattaaa
      841 tagctctttt gtatccatat ttaggtaatg tgtagcatta gctacatttt tcattaaata
      901 tcaactccta aatatttagt catttttatt tttgtccctt gattatgttc tgattcaata
      961 tctacatcat ccattaaagt tttaattata aacaatccaa gaccactctc ttttggattc
     1021 tctaaatctg gtgtacttat tgagctaaca tcatatcctt ttccattatc ttgaatttct
     1081 atattaattg cattatctaa aatattaaat actatattaa acacatcatc tttactgtgt
     1141 tttattgcat ttgtacatgc ttcagatact gctaccttta tatcttctat atcatctatt
     1201 ggaaaaccca tcttattggc aattccagat gctgttaatc ttattatact aacataatct
     1261 ggatttgtag taatttccat ctttatagtc tcacaagcca aagtttatcc ctccacttta
     1321 aatattttat ctagacctgt tatagtaaaa atctttttta cgttactctt aggatttact
     1381 atgtatatct ctttttcatt tatttttaat ttttttaata cacctatcat agctcctaag
     1441 cctgttgagt ctatataatc taaatcttta agattaatct ttacatctaa catatttttt
     1501 tctattaaag catgtaaatg ttcctttagt ttatctgctg tagaaacatc aagttctcca
     1561 tcaagacaaa cattccaaaa tttattttgt gaatctaaat ttgaatcaat attcatagac
     1621 atgtaaaaac ctcctatttc cttttaataa ttttaaatat atttcctata ctcaatattt
     1681 tattaattac ataaactata tcatctattc cctttgatat tgatgatgca ttttcaacaa
     1741 tatcttgtat atgtctttca ttataatcta caatcttata tgccttttca acaaccttat
     1801 ttatgctgtt tagagtttta gctaaataaa ttgctactat aagagcagag cttcctacta
     1861 acactgctcc aatttgccac ccccatgcat tcattagtta acatctccct catcatcaaa
     1921 gcttttactt attactatat cttcttctga aatctttata tcttcttcat tattatcatc
     1981 gtccactaaa tttattattc tctctttaac atcattaaat gtttcatgta gaacttcttt
     2041 tggattttct ttgacatcat taaacttctc ttttgtttct tttcttagtt cagaaccttt
     2101 tttaggtgca aaaaacatac caaatataaa ccctaaaata gctcctaata ataaaaatcc
     2161 acctttttta caacttcttt tattgcacat aacaattact cctttcaatt ttaaattttt
     2221 atctaactac attatacaaa aagaatagga tatttatcct attcttcttc aatatttatt
     2281 ttggcaatcg aaactacatt gacttcatca ttcgtcttca ttaatttaac accacttgta
     2341 actcttccaa acagtgatat ttcattgact cttattctaa taagaactcc atcagaattt
     2401 ataatcatta tctcatcatc ttcgttgacc atatctgcac ctacaattgt accagttttt
     2461 tcagatatat tataagtttt aattccttta ccagctctta tttgacttct atactcttct
     2521 atatttgttc tctttccaaa accattctta cttacaacta acacatctgt acctttactg
     2581 caaaggttca tagacacaac aaaatcttct ttacttaaag ttataccttt tacacccatt
     2641 gctgttctac ccatatatct tatatcattt tcatcaaatc ttatagacat accttcttta
     2701 gttactaaaa gtacttcttg ttttccatct gtaagttcta ctccaataag ctcatcatca
     2761 tctcttaaac ctatagcaat aagaccagat ttatttatat ttttgaactc ttctctctta
     2821 gtctttttaa caataccttt tttcgttgca agtaataaat attcattttc atcattacca
     2881 tcaataggta ttagagtagc aattttctca tctgctgaca attgaagtaa attcactata
     2941 gcagtacctt tagcctgtct cttaccttct ggtatttcat atgcatttaa tttaaatact
     3001 ctacctttat ttgtaaagaa taatagtcta ctatgagtag ttgtagttac caagtgcctt
     3061 acaaaatctt cttctctagt tgtaagtgct gaaatacctc ttccacccct tttttgactc
     3121 ttataagtat cagatggaag cctttttata tatccaaagt gagtaagagt tattgctatt
     3181 tcttcatcac ttataagatc tctcatatct atttcgcctt cagcatgtct tatttctgtt
     3241 cttctctcat cagagtaatt ttcttttatt attgtaattt cattctttat tacatttaaa
     3301 agtaatcttt catcagctaa aatttctttt aatctattta tcttcttgat taaatcttca
     3361 tattcagctt ctatcttatc tctttctaaa cctgtaagtc tttgaagtct catatctaat
     3421 atagcttgtg cttggatttc agttaatttg aatttttcta ttaaacctat cttagcttct
     3481 tgcccagtct ttgaagctct tatcaagctt ataacagcat ctatattatc taaagcaatc
     3541 tttaatcctt ctaaaatatg tgctcttgct tcagctttat ttagttcaaa tttagttctt
     3601 cttgtaacaa catcttcttg atgtttaata taatgatata atatttgttt aagatttaaa
     3661 actcttggct gaccatctac aagtgcaagc attattatac taaaagtatc ttccatttga
     3721 gaatgtttat acaaattatt taatactata ttagcattag catccctctt taattctata
     3781 acaattctca taccatttct attactttcg tctcttaggt ctgatatacc ttctattctc
     3841 ttttctttaa ctaactctgc tattctttca accagtttag ccttatttac ttgataaggt
     3901 atttctgtaa ctattatttg ctgttttcct tttggtagtt cttctataaa agctctagac
     3961 ctaactttaa cttttcctct tccagttctg tatgcttctg ctatactttc ttttcccatt
     4021 ataattgcag ctgtagggaa atctggtcct tgaacaaatt ttattaaatc atctacacta
     4081 cactctggat tatctatcaa atatacagtt gcatcaatta cttctgctaa attatgtgga
     4141 ggtattgaag ttgccatacc aacagctata ccatttgagc catttactaa taaattagga
     4201 tatctagctg gcaatactga aggctctttt aacgactcat caaagtttgg tttaaagtct
     4261 acagtttcct tttcaatatc tctcagtagt tctaatgata atttactcat tttagcttct
     4321 gtataacgca tagcagctgg tgaatcccca tcaacagaac caaagttacc atgaccatct
     4381 actaaaagtg ctctagttga aaaatcttgt gccattctta ccatagcata ataaacagca
     4441 atatctccat gaggatggta cttacctaaa acgtccccaa caatacgagc tgacttcctg
     4501 tatggtttat ctggagttaa atttaactca ctcattgaat ataatattct tctatgaact
     4561 ggctttaaac catctctaac atcaggaaga gcacgtccag ctataacact cattgaataa
     4621 tcaatatacg attttttcat ttcttccgct atttcaatag ggagtatttt gttattttct
     4681 tccatgtata ctaatcccct ctctctatat atctaaattt ctaacaaatc ttgcattttc
     4741 ttctataaat tcttttcttg gagcaacctt atcacccata agcattgaaa atacttcatc
     4801 tgcaattgca gcatcttcta cagtaacttg taataaagtt cttgtctcag gattcatagt
     4861 tgtttcccat aattgctctg cattcatctc tccaagacct ttgtaccttt gaagttcaac
     4921 accatttctt ccaatttcat ctaataagat atttaactct ttatctgagt aaacataatg
     4981 ttccttcttt tgctttgtca ctttatataa aggtggctgt gctgcataaa catacccctc
     5041 atctataaga ggtctcatat atctaaagaa gaatgttaat aataaagttc ttatatgggc
     5101 tccatctaca tcagcatcgg tcataattat aattttatga tacctagcct tatctatatc
     5161 aaaatcttct ccaataccac aaccataagc tgttatcata ttttttattt catctgaaga
     5221 tagtatccta tctagtcttg atttctcaac attaagtatt ttccctctta atggaagtat
     5281 agcttgacta tttctatctc taccttgttt agctgaacct cccgctgaat ccccttcgac
     5341 taagaatatt tcacttttag atggatcttt ttctgcacaa tctgcaagtt ttccaggtaa
     5401 agatgtactt tctaatacac tttttctcct tgttaattct cttgcttttt ttgcagcttc
     5461 tctagctctt tgtgctctta aagctttatc aactatgatt cttgctgttg atgggttttc
     5521 ttctaggaaa gaccccagtt catcaactgt tacactatca actatacctc tcataaagct
     5581 attacctagc tttgttttag tttgaccttc aaattgaggt tctggtaatt taactgatac
     5641 tactgctgta agaccttctc ttatgtcttc accaagtagg ttaacatcat tttcctttaa
     5701 aaatttattt ctcttagcat aatcatttac agttttagta agagctgctt taaatccact
     5761 taaatgagaa ccaccttcgt gtgtatttat attgtttgca aaagaatata tgttctctgt
     5821 ataaccatct gtatattgca tagctagttc aacaaaacaa tcatcaacct ttttatctat
     5881 ataaactatg tcatcatgta taccagttct agatttattt aaatacttaa tatattctac
     5941 caaaccacct gtatagtgaa attcttttct tttttcttgt ccttctcttt tatcttcaaa
     6001 tactattttt attcctttat tcaagaaaga aagttctcta agcctatact ccaatgtttc
     6061 atatttaaac tctatttcat caaatattgt ctcatctggc ataaattgaa tcattgttcc
     6121 tttatcttga gattctccaa ctacttcaag cttagatgtt ggtaatcctt tctcatatgt
     6181 ctgcttatat atctttccgt ttaaataaac ctctgctacc atccattttg acaatgcatt
     6241 aactacagaa acaccaactc catgaagacc tccagatact ttatatcctc cgcctccaaa
     6301 ctttcctcct gcatgaagat tagttaatac tgtttctaaa gttgatttac cagttttagg
     6361 atgtatttca acaggtatac ctcttccgtt atcttttact aaaacactgc catcttcatt
     6421 aatagataca tatatgtctg aacaatatcc ttgtaaagcc tcatctatac tattatctac
     6481 aacttcataa actaagtgat gtaaacctct agggcttgta ctaccaatat acatacctgg
     6541 tctttttcta acggcttcta atccctctaa tacttgtatc tgacttgcac cgtattcttg
     6601 tttcatttaa tttcctccat tctctatgct aataacatcg ccattttcaa tattaaatat
     6661 tgtaacattt ttttcatcaa atattttttt atgtgaaact tcagcacttg ttataaacat
     6721 ttgaacattg cttaaattat ttactaaaag tttttgtctt gcttcatcta actcactaaa
     6781 aacatcatct aaaattaata ctggatattc ttcaacttca ttttttatta attctatctc
     6841 tgataacttt aatgatattg aagcagttct ttgttgacct tgtgaaccaa aaagtcttgc
     6901 atctaaatca tttatgaata tatttaagtc atctttgtgt ataccatatc tggtagtctt
     6961 agattctata tcattaggtc tattcgatga caacttggct aaaaatttat tatatactgt
     7021 atctatagta tcttcatcag ttatatttat ttgattttta tatcgaattg ataatctttc
     7081 aactccatta gtcaaattca tatgcatatt ttcagatata ttagctatct tttttataaa
     7141 gtctctacgc aaaatataaa tataacttcc atactttgct aacgtatcat catatacatc
     7201 taataaagct tcatcaacat gtatattttt aagcacccta ctcctttgag aaagtgtttt
     7261 attataattg gtaagatatt tataatattt aggtataatc tgactaatct ctttgtctat
     7321 aaatgccctt ctttctttag gaccctcttt aaccaacctt aaatcttcag gagaaaatat
     7381 aacaacattt aagttgccaa gtagttcttg tatcttttgt aaaggaactt tatttattct
     7441 tatccccttt ttatcttttc caataattag ctctattaga ctatatttat tatattttga
     7501 aaaacttcct ccaatgtata agttttcact attaaatcta accatttctt tatctttgtt
     7561 agtcctaaat gattttccaa aagaaagcat ataaatagat tctactatat ttgtctttcc
     7621 ttgaccattt ttaccaacaa gtagatttac tttcccatta aattccagat gcaacttttt
     7681 ataatttcta aaattaacaa gttgcaaact tttaagtttc acaaaaagtt ctcctttttt
     7741 tacaatactt ttattttagt tccttctatt ttaactatat ctcctgattt tatctttttt
     7801 cctcttctta attctatttc atcatttact gtcactaaac cttcttgaat taaaaatttt
     7861 gcatgacctc ctgttgatgc aatctctgct aatttaagaa attgatccaa ctttatatat
     7921 tctgattcta tagttatttc agtcataata gttcctcctt gtcactattt taaacaaatt
     7981 cctattgata cactaaaagt atattatacc ataaatatgc ttataaaagg agttgtctaa
     8041 gattagaaaa attttctaca aaagacaact ccttcatgtc tgctatatat ttgatgatat
     8101 tcttactgga agcagtaagt aaatataatt aacatcatct gttggtttaa ttatacaagg
     8161 atttacattt gtagtaaatt ctataaatat ttcctcatta tctatatttt tcaaaccttc
     8221 gataaaatat ctagaattga aagctatatc taggtaatct ccatctaaat ctatttcaac
     8281 ttcttcatac acatttcctt tttctgtgtt agaagtaatt gccattactt tatctcttat
     8341 agataacttt ataagattat ttttttctga ttgagacaat aaagacgctc tctctatact
     8401 atttaaaagt tccttagtgt ttaatttaac tctgctgtta tgttctcttg gcaataattt
     8461 tttataatca ataaaatctc cttcaagcaa tcttgtaata attttcgtat cattaattat
     8521 aaaaatagca tttttttcgt taaacccaac tttaacatta tcctctccag aaagcaaact
     8581 atttacatct ataagtgtct ttccaggaat tatgactttt atattttcag ttaaactatc
     8641 aactgaacaa ctttttacag ctaatctata tccatcaatt gctactaaat ttaagtttct
     8701 atctacaatt tctaaaagtt ctcccattaa aacaggtttt gtttggtcct gtgagatagc
     8761 aaatacagtt tgtttaatca tattttttag aatttcttga ggtatgctat ataaatcttc
     8821 ttcatttaat tctggtaatt taggaaattc ttttgctgca taacctttaa ttttaaatct
     8881 tgaatttaca caatttatgt aaatattatt ttctgaatca gtttctatct caacaaagga
     8941 atctggtaat tttctaatta tatctccaaa tagccttgca tctacaacaa catcaccttt
     9001 ttctatgatt tctgcttgta cataagtttc tattcctatt tcagcatcat atccagtaag
     9061 ttttaattga ccttcttctg tagatataag tatacctttt aataattcta ttgttgtttt
     9121 tccattaata gctttttgag caattccaat tctatttgct aggatttttt gattacaaat
     9181 tattttcaat gtggataacc tcctctggct ttttatatat aaaaataagt taaaatcata
     9241 gtagtaatag tagtaggtgt tgttgataat gttaataagt atgtataaca ttataaatac
     9301 aaagatatta acatgtgtat aagatgttaa tatctttgtt attttagact tgataatttg
     9361 ttaataattt ttgttcaaaa aaagtattaa caggttattc aagagatata aacatacaat
     9421 tgtgtataaa ttatcccttc aaatctgata taattttgtc tattttagtc ttaatttctt
     9481 cactttcttc tatatcctta gatactttgt catgagcatg aattactgtt gtatggtctc
     9541 tacccccaaa ctcttcacct attttaggaa gggataaatc agtaagctcg cgcgttaaat
     9601 acatagctat ctgtcttgga taagctattg aacgtgttct ttttttagaa ttaaaatctt
     9661 ccatctttaa attgaataca gaagatactt tttcttttat tctaagaaca tttatttctt
     9721 catttttaga agttgttata atatctttta aagcttctgt agcaagatca aaagatatta
     9781 ccctattgct aagagaagaa taagcaacca ctctagttaa tgcaccttct agttccctta
     9841 tgtttgactt aatatttttt gctatatatg acataacctc atttggcaca tctatacgtt
     9901 ctagttgagc cttttttcta agtatagcaa ttcttgtctc aaagtcaggt gcttgaatat
     9961 cagttataag tcccatttca aatctcgatc ttagcctatc ttctagtgta ggtatgtctt
    10021 ttggcggtct atcactagat ataattattt gtttgtttgc ttcatgaaga gtattgaatg
    10081 tatggaaaaa ctcttcttga gttctttctt ttcctgctat aaattgaata tcgtcgatta
    10141 gtaatacatc tacgtttcta tatttattcc taaattcttc gtttttatcg tcttttatag
    10201 agtttataag ttcattagta aatttttctg aagaaacgta aactactttt gagtcttttt
    10261 tttgactaac aatatgatga cctatagcat gcattaggtg agtcttacct aatccaactc
    10321 ctccatacaa aaaaagtggg ttataagctt tagctggaga ttctgcaact gcaacgcatg
    10381 cagcatgtgc aaatctattg ctattaccaa taacgaatgt atcaaatgtg tattttggat
    10441 ttagattggg gtaaagaact ctataattta aatctgtgct atcagaattt aaatcagcaa
    10501 cctctttttc gctcaataca aatttaatat tatatttttt taatgacagt tgactaatag
    10561 catcttcaat taaatgtaaa tatctattct ctaagatatc tttattaaaa tcactaggag
    10621 ccagtaagat taaatcatta agatgtattt ttaatggtac gatgttttta aaaaaggtat
    10681 taaaacttac tgaagttaag tcacctttta ttaattgtag ggttttgtcc cataaagaaa
    10741 ctatatccat aatataaacc tccgaacaga ataattaata ttttccacaa agttattaac
    10801 agaaaagcaa aaatatatat aaattttaca gaataactaa atttatggat aatggtgttg
    10861 ataaattaat aatagtataa attattaaaa aaatatgaaa agatataaac aatatgtaga
    10921 taaagttata aataacataa acatacaata aatggtgatt ataactagag tattgtcagt
    10981 aatcttaaaa gttatacaca aaagtatcca caagctgtga ataatttcta ataagttgat
    11041 aacatttatg cacattatca acaagtatat ttataatatc aaaatatatg tcaaacttca
    11101 atgatttgta actaatttat ccacaatatt aactaattgt tgataaaaat ataaacatgt
    11161 taaaattact ttatttgtgt atataattga aaaaaataat ttagaataaa attaaataga
    11221 tttttgctag tattttcaat aaaaaaaata cataatatat atatggtaga ttttgtgata
    11281 taagcgtccc aaaaactcgt tgacaacagt agtaaaagta tttataatta tatgtgttat
    11341 gtttactttg agggtaaagt tattattttt ataaacggaa aataaaatct gaaaggcggt
    11401 gcaaaataat gagtaaaaga acttatcaac caaaaaagag acaaagaagt aaagaacatg
    11461 gtttcagaaa aagaatgaag acgtctaacg gaagaaacgt gttaaagaga agaagagcta
    11521 aaggaagaaa tagattaact cattaattac aaaagggccg ctgcaaaggt ggcctttttg
    11581 tgtaaataat tacaattcat agctgtgttt atggaggtta actatggact ttaataggac
    11641 taaagggttg aaaaaagact ctgattttag aaaagtatat aaacacggca aatcttttgc
    11701 aaacaaatat ttagtaatat atatacttaa aaataaatca gattatagta gagtaggtat
    11761 ttctgtttca aaaaaagtag gaaaagctat tactaggaat agagtaagaa gattaataaa
    11821 agaagcctac agattaaata ttgatgaaaa aattaagcct ggatatgata tagtatttat
    11881 agcaagggta tctagtaagg atgctacttt taaagacata gacaagtcta taaaaaattt
    11941 agttaaaaga acggatattt ctatttaatt gtatgctata aatatggagg ttaatgactt
    12001 tgaataaaat aatttatata ttaaaggaaa taagtaggta tttatcgaat ttatgtattt
    12061 atttagtgag attttatcaa aagtatatat ctcccttaaa gggacctacc tgtagatttt
    12121 atccaacttg ctcacaatat tcaatagagg cgtttaaaaa atatggattt gtaaaaggta
    12181 tgtatttaac gataagaaga cttttaaaat gtcatccttt tcatccagga ggttacgacc
    12241 ctctaaaata aaagagagta actattagga ggtataaaaa aattgaatat aataggaaat
    12301 ttcttaggat tgattcttaa ggtaatattt gaatttgtta atcattatgg tatttctata
    12361 atacttttta cgatacttgt aaagattatt ttattaccat taacaattaa gcagactaag
    12421 tcaacaaagg caatgcaaga tatacaacct cgtataaaag agatacaaga aaaatataaa
    12481 aataagccag aaaaacaaaa tgaagaaatt gttaagttat atggagaagc taaaattaat
    12541 ccactatctg gttgtttacc tttattaata cagtttccta tattaatagg attatttagc
    12601 gttttgagag aaccagttgc tcatggcgtt tttgcaaaca aagctgcatt tttagcagct
    12661 gacaatgggt ttttatggat aaaaagctta acatcaccag attatgtatt agcagtattt
    12721 tcaggagcaa gtgcatatgt catgcaaaaa gtaatgacac ccaaagatca gttacaaggc
    12781 tctatgaaag taatgacata tgtaatggca ggtatgtctt tctattgggg atttatattc
    12841 ccagcaggac ttacattgta ctggacagta agtaacttat tctcaatagc tcaatattac
    12901 ttaataatga atcctttaaa agctaaactg gctgctaact ctaaggagga aaaagttaat
    12961 gagaaaaagc ctaaaattaa taaaaagtaa gagtaaagag gaagctataa gtaaagctat
    13021 agcagagttg aatttaaaag cagaggatat agaagtagaa atacttgaaa atccaagtaa
    13081 agggttttta ggttttatag gtgctaaaga tggtacttat gaaatttttg ttatcgagag
    13141 ggaagtagat gtagctaaaa attttataga ggtaatgttg aaaaatgcaa atgttgatgc
    13201 caaagtcaat gtatctcaaa aagataattt aatcatggtt gatattgaag gcaaagaagc
    13261 tgcaagttta attggtagaa gaggggaaac tttagattct attcaatttt tgactggatt
    13321 agctcttaat aaaataaata aagattctca tacaagagta cttgtagata tagaaaatta
    13381 tagatcaaaa cgagaagaat ctttaataag atatgctaat aaagttgcta gagaagtagc
    13441 gaaaacaaga aaaactaaaa aattagatta tatgaatcca tatgagagaa gaataataca
    13501 ttcagctctt caaaatgaca aatatgtaat tacatatagt gaaggaaccg atccatatag
    13561 aagattagta attgagtgta aaagataaag tcaacctcct aaacattagg gggttttatt
    13621 ttttaatatt attaaatcat taatgattgg ttaaatgtaa acctatatgt aaaaaattat
    13681 gaattaagag gtgataaatt tgtttataga tgatacaatt gcagcaatag ctacagcacc
    13741 tggagaaggt ggtataggta tacttagaat aagtggagaa agagctttga aggttgcaga
    13801 agaaatcttt aaatctatgt cgggaaagag tattgaagaa tacaataaga gaacattaat
    13861 atatggaaat atattggata atgaaaatat aattgatgaa gttttattgg catatatgaa
    13921 aggaccaaac tcctatacag gagaagatgt tatagaaatt aattgtcatg gtggatttat
    13981 ttcggtaaag aagatattag aattaatact atcaaaagat gttagattag cagaggctgg
    14041 tgagtttact aaaagagcat tcttaaatgg aagaatagat ttatctcaag cagaggctgt
    14101 aattgatgtt ataaaagcca aaactgatat agctcatgaa gttgctcaaa atcaattaga
    14161 gggttcttta tcaaagaaga taagagaatt gagagataaa gtcactgaga tattagctca
    14221 tgtagaagtt gctatagatt atcctgagga agatattgag catataacat atcaaactct
    14281 taaagaaaaa actgatgaac ttaaaaaaga gattaaaaaa ttatatgata cagctgaaag
    14341 tggaaaaata cttagagaag gcttaaagac ggtaattgtt gggaaaccaa atgtaggtaa
    14401 atcttcacta cttaattcta ttttaggaga gaatagagca atagttactg atataccagg
    14461 aactactaga gatgttatag aagagtttgt aaacataaaa ggtatacctt taaaaattgt
    14521 tgacacagca ggtataaggg atactgatga tatcgttgaa aaaataggtg ttgaaaagtc
    14581 taaagaatca tttactagtg cagatttgat agtaatggtt ttagatgcat ctagaaagtt
    14641 gtcagaggaa gatatagaga tattagaaaa acttaaggat aaacaaacaa tagtgctgtt
    14701 aaataagaat gatttgaaac aagagattga agaagaaaaa atacttaaat atgtagaaaa
    14761 taatagtata ataaaaatat cagctttaca acaagaagga atagaagaat tgcaagataa
    14821 aatagagtct atggtatata aaggtagtat caaaaataat tctagtttag tagtaacaaa
    14881 ttcaagacat aaagatgcac ttagtaaagc atataaatct gcaacagatg ctttaattgc
    14941 tttggaacaa agtatgccat ttgactttgt agaagtcgat ttaaaaaata tatgggacta
    15001 tcttggatat attaatggtg atacagtaac agaggattta ctagataata tattccataa
    15061 tttctgtatt ggtaagtaat gtacaaaagt aaatttgttt ttattataga gatttttaaa
    15121 aaatttgaag tgtttctcat gaaacattaa ttttgaaaag aaaggaatta taaatgataa
    15181 agtttgaagc aggaaaatat gatgttatag tagtcggagc aggacatgct ggatgtgaag
    15241 ctgctttggc tactgcaaga atgggatata aaactttgat aataacaatg tctttggatt
    15301 ccatagcatt aatgccttgt aatccatcaa ttggagggac tggtaaaggt caattagtta
    15361 aagaaataga tgccttaggt ggtcagatgg gattaaatat agacaagaca tatatacaaa
    15421 gtagaatgtt aaatactgcc aaaggtccag cggtacactc tttaagagca caagcagata
    15481 aatttaaata tcatgaagaa atgaaaaaga cattagaaga tgaaccaaat cttgatatag
    15541 ctatggatga agttgtagaa atacttcatg aaggaaatgt tgtaatagga gttggaacta
    15601 aactaggatg ttcatttaaa tctaaagctg taatattggc cactggagtt tatctgaact
    15661 caaagatata tatgggtgaa gttgcatttt atgaaggtcc taatgcattg gggtatgcaa
    15721 aatatcttac agatagctta gttgagcttg gtcttagaat gagaagattt aagacgggaa
    15781 ctccagcaag agttcataga gatagtatag acttctctgt aatgtcttta caagagggcg
    15841 atgaaaaagt aactccattc tcatttatga atgaaaatat agaaaaaaaa caagaacctt
    15901 gttatttaac aagaactaca gaagaaactc aaaaagttat actggataat ttaaaaaggt
    15961 ctgctatgta tagtggagta atagaaagta ctggtccaag atactgtcct tctatagaag
    16021 ataaagtagt aagatttagt gataaaacct cacatcaatt atttatagaa ccagaagggt
    16081 taaatactaa agaaatgtat atacaaggaa tttcaactag tttacctttt gaagttcaat
    16141 tagatatgta caaaactata aagggacttg aaaattgtaa gataatgaga cctgcatatg
    16201 ctattgaata tgattgtgtt gacccaactc aactaaaaat aagtttggaa ataaagggtg
    16261 tcgagaactt atttagtgca gggcaattta atggaacttc tggatatgag gaagcagcag
    16321 ctcaagggct tatggctggt ataaatgcag ttagaaagat agaaggaaaa gagccgtttg
    16381 tattagaccg ttcagaagct tatataggtg tcttattaga tgaccttgtt actaaaggaa
    16441 ctaacgagcc ttacagaatg atgacatcaa gagcagaata tcgactatat ttaagacaag
    16501 ataatgcaga tatgagatta actcaaaaag gttatgatat aggattagtt aaaaaagata
    16561 gatatgaaag gttcctaaat aaaaaggctg ctgtagataa agaatttgaa agattaaaaa
    16621 atgaaagagt tacaccaaaa gaagtaaata gtttgttaga agaaaaagga gcaacaccaa
    16681 taaaggttgg aatatctcta tatgaatttt taaagagacc agaggttact tacgaattgc
    16741 ttgaagaatt agggaagggc gctggagaag atgtttcaag agaagttaaa gaacagtgtg
    16801 taataataac taaatatgaa ggttatatag agaaacaatt gaaacaaata gaccaattta
    16861 aaaaacttga gaataaaaag ctggatgaga aaataaatta ttcatctata gaaggtctta
    16921 gattagaggc tagacagaaa ttagatgata taaaacctat ttcaataggg caagcatctc
    16981 gtatatcggg tgtttctcca gctgatatat cagttttact tatataccta gaacaagtca
    17041 gaagaactag aggtggcaaa ggtgaataat atagaacttc tgaaaaacgg gattgaaggt
    17101 tttaatatag atacgaatga cagtatgtta gaaaaattta aaatctatag agagatatta
    17161 gttgaatgga ataaaaagat gaatcttact ggaatagaag atgaaaaaga agtgtatata
    17221 aaacattttt tagactctat ttcagctgta aagaatggat atataaaaaa tgggatgtct
    17281 ataatagatg ttggtacagg tgctggtttt cctggaatac cattgaaaat atgtttagat
    17341 agcttagaac ttactttgtt agattcgtta aataaaagaa taaacttttt agaggaagtt
    17401 agtagagtac tagagttaga taatattaca tttatacatg gaagagctga agattttggt
    17461 aaaaatgaag actatagaga aaaatatgat gttgctacgg caagagctgt tgctggatta
    17521 ccgattctta tggaattttg tgtaccattt gtaaaagttg gagggtattt tatttgctta
    17581 aaaggtccga atgctaattt agagctagaa gaatcacaaa aggctattga tgttttagga
    17641 ttggagtata ttgaaaaaat agatgtagag ttaccagagg tagatttaaa tcataatata
    17701 ttagttttta aaaaggtgat ggaaacacca gttaagtacc ctagaaaagc tggtagacca
    17761 gctaaaaatc ctattaagta atgaaaaaag taatgaaaaa attattattg gtaagcttaa
    17821 ttatttctta ggaaataatt aagcttaccg taaaaaaaca aaaataaagg aattattaaa
    17881 gtagatgcag aaataaattt gtatgtacat atagagaaca aaaataattt atatctaaaa
    17941 taagagaggt atgttatgga agataaaaga gttatggaga tacctataga agatgtagtt
    18001 ccaaatccat atcaaccaag aaaaatattt tcacaagttt cattggagga attgagcaat
    18061 tctataaagg tatatggtat aattcaacct ataacagtta gagcaaaaga tggcaaatat
    18121 gaactaattg caggagaaag aagattaaga gcagctaaat tagcagagct taaaaccata
    18181 ccagcaatta taaataatat gaatgatgaa tcttctgctg ttctagcgtt attagaaaat
    18241 ttgcaacgag aagacttaaa tttcattgaa gaagcaattg gttatgaaaa cttaataaaa
    18301 gaacatgctt ttactcaaca gcaattagca gaaaaattgg gcaaaaatca atctactgta
    18361 gcaaataagc ttagaatatt aaagcttcca aacgatataa aaatgaagct aatagagaat
    18421 aatttaacag aaagacatgc tagagcattt cttaaactac caagtgaaga tttaatgcaa
    18481 agtgtgttag acaaagtcat aaaaaatgaa ttgactgtta aaaaaacaga aaaattgata
    18541 caagatgtat tagaagaaac taaagttcaa gaagagcctg ataaaaaaca gaatataaag
    18601 ggtgctatga gtataagaat ttacataaat actataaagc aggcctttga tgctatatca
    18661 aatacaggaa ttgatgctaa gtataatgaa atagataagg gagattatat ggaagttgtt
    18721 gtgaaaatcc ctaaaaaata gagattactg cttaccagta atcttttttt gtaatataag
    18781 ccttgtaata taaattgatt tatagacatt atttatactt aaaaaacacc tattcaacat
    18841 ttaaaattaa tttaattctt aaagggggga acatcatggg caaagttata gctgtattca
    18901 atcaaaaggg tggagtcggg aaaactacaa caaatgtaaa tttaagtgct agcttaggaa
    18961 ctttgggaaa gaaaatatta gtattagatt tagacccaca aggaaataca actagtggat
    19021 atggtattaa taaaaacgaa gtagaaaata caatctatga aattatgtta gatgggttac
    19081 atattaaaga agcaataata agtactgaat ttgagaatgt agatgtagtt ccatcagcta
    19141 ctgaactatc aggtgcagag atagagctta catctaaaac taatagagag tatattttaa
    19201 aaaattccat aaaagcggta atagatgaat atgattatat atttttagat tgtccaccct
    19261 cactggggat gcttacaata aattgtttaa ctgctgtaga tagtgtttta attccaatac
    19321 aatgtgaata ttatgcttta gaaggtgtta gtcaattaat ggagactata aaattagtaa
    19381 aatcaagatt aaatgctgat atagaaattc aaggtgttgt tttaagtatg tttgatggaa
    19441 gagctaattt atcaatacaa gtagttgaag aagttaagaa atactttaaa ggtagtgtat
    19501 atacaacttt aattccaaga aatgtaagac ttgcagaagc tccaagtcat ggaaaacctg
    19561 taatttatta tgataaaaga tgtagagggt ctgtagctta tttggagcta gcagaagaat
    19621 ttatagattt agaagaggag gaatggtagt atggagaata agtctaaaag aagtaataga
    19681 ttaggtagag gtcttagtgc attgatacca gaaattaaag gagaaacttc agaaaaagaa
    19741 atagtcaaca tagatataga caaaatatat ccaaacgaag ttcaacctag aaagcaattt
    19801 gatgaagaaa agataaaagt attgtcggac tctataaaaa attatggtgt tttacagcca
    19861 atagtggtta aaatggatga aaataataaa tatatgataa tagctggaga aagaagattt
    19921 agagcatcaa aacttgcaaa taaaaatcaa atacctgcta taattaaaga tatagatatg
    19981 aaagatataa tggagatagc cttaattgaa aacttacaga gggaagattt aaattcaata
    20041 gaagaggctc tagcatataa gagtttaata gagcattaca atgtaactca ggaagaaata
    20101 tcagaagctg ttgggaagag tagacctcat ataactaata cactaagact tttaaatctt
    20161 ggacaagatg ttatagaaat gatagacagt ggtagaatta cagcaggcca tggtaaggct
    20221 ttattgagaa ttgcagataa agatttgcaa cttcaaatag cgaaaaagat agaagaagaa
    20281 gaactttctg taagagaagt cgaaaatata gctaaaaaaa tatctgaaaa taaacaagaa
    20341 gaacctaaaa aatctaaacc taaggatgta tttattttag atgttgaaga taaacttaga
    20401 aatatatttg gaacaaaggt gaatatttct aaaggaaaga agaaaggtaa aatagagata
    20461 gaatactata atgatgatga tttaaatagt attgtatcaa tgcttcttga atagaacaaa
    20521 aatataaaac agagaggaag attgttatga tttatttaga taatgctgca actacatatc
    20581 caaaaccaga aagagtgtat aatgctgttt tagattgtat gaaaaattat tgtgcaaatc
    20641 caggtagagc aggccataaa cttgctatga gagctgctag ggagatatat gatacaagag
    20701 agaatatagc taagttgttc aatgtaagta atcctatgaa tatagtattt acatctaatg
    20761 caacagattc tttaaattta gcgataaaag gtgtattaca agaaggagac catgtaataa
    20821 caacgtctat ggagcataat tcagtaataa gacctataaa agcattggaa aaaaggggta
    20881 ttgaaaatac tgtagtaaaa tgcgattatg agggattttt agattatgaa gatttagaaa
    20941 aatcaataaa gagtaatact aaactgattg taacaacaca cgcatctaat gtatgtggaa
    21001 ctttaataga tataaaaaaa gttggagaaa tagctaaaaa acataatata ctatttttag
    21061 tagatgcatc acaaacagca ggtgtttatg atatagacgt aaatgaatgt aatatagata
    21121 tgttggcaat gccaggtcat aaatgcttat ttgggcctca aggaacaggt atattatatg
    21181 taagagaagg gttgaactta aatatattaa aagaaggtgg aacaggtagt aaatcagaag
    21241 aaatagttca accagagtta ttcccagata agtatgaatc aggaactcat aatactccag
    21301 gtatagcagg tcttaaccaa ggtatactat ttatatttga aagaggaatc aacaatataa
    21361 gacaacatga agaagaatta tgtcaatata tgatagacaa actagaggaa gtatcagata
    21421 taaagatata tggaccaaag gatagtaaaa aaagagcatc agtaatcgct ctaaatatag
    21481 gagatatgga ttctggagag gttactttct tattagatag tgattataat atagcaacta
    21541 gatcaggaat acattgttca ccactagcac ataccacttt aggtacttta aaacaaggtg
    21601 cagtaaggtt cagtatagga tatttcaaca caaaagatga gatagataaa gcagtggaag
    21661 ctttgaaaaa aatttcaaaa aataaatagc atgagattta ataatataga aataaaaaaa
    21721 ggggatattt caaaataaaa atttgaatca tctccttttt tgtacaaaaa agaaaagggg
    21781 ctcctaagaa cctctaatct atttgataat tgtttcaaga aattacttta agtaatctcc
    21841 ttcttcaaga actatatgac cactttcgta attagaacca tcaacagtaa tttttatttg
    21901 ttcaacattc atatttttta tgtaagtacg agcaactgaa tcaagcatta aagtttctgc
    21961 atcactacct aagtttgact ttgtaaagtc agagctaaca tctaatatac cagtttttac
    22021 accatcaatg tcttttgtag taaatgaatt taattttgca gattctggaa caactttaag
    22081 tttttgaagt tcttcaaata aagtgttctc atcaatttta tttaaatcaa ctttattttc
    22141 aattaaatct gtcttatcta catcaaaaga gtaaatagta gctgattgag ttttagtttc
    22201 ttcttttgga gtaggtttag ctttgttatt aggaacctct ttttcagtat tgttttcttc
    22261 ttttggttgt tcaacatttg tattgctctt agtttctttt ttagatgtat cacttccatt
    22321 ttgagcatta gtacaaccaa ctgcgaatat agatatagat aatatactta acactaacat
    22381 tattttctta tttttcataa aaatttctcc tctcttaaaa actattctta tatataatta
    22441 tagtcgttat gagcaaatat atgaatacaa aatagtaaca aatttaaaaa atgatttatt
    22501 taaattaata aaactcaaaa taaactaaat tttagtcatt tttatgggaa atcatttaat
    22561 gtacagtgaa aatattgtaa aataaaagta gtgtttttaa aataaatata agttataatt
    22621 atattataaa aaaatgtctt caaaaggtgg gaatagtatg gaaattttga gcttaggaga
    22681 aaagattaaa aaattaagaa aagaaaaaaa catgacatta aaagaattag ctggagatag
    22741 gataacagca gcacaaataa gccatataga gagggataaa tctcatacta gttatgaact
    22801 gttggaatat ctaggcgata agctagatgt aagtgtagaa tacttattag agaccaagga
    22861 aatgcaatca aaaaagataa ctgataattt aatactacag agcgaaattt atataaaaga
    22921 aaatgaatta aacaaggctg aggatcaaat aaaagaagtt ttagatatat gtaagagata
    22981 cgatttaatg gagaactatg gcagatgtaa ttttttaatg gctaatatta atttgaaaaa
    23041 atctaattat aatgatgcta tagaaaactt tgaaaaggca ttgtacttct ttataaaaaa
    23101 tagtgatagt gaaaatatac ttaagtgtta tttaaacata gggaaaatat atatgcagga
    23161 agacttttat aaaggtgcta taagccactt taattttgca gaggaaattt tgtcagagaa
    23221 taaatttgag gatgttaaca tacacaagga actatatagt aagatggcat attgttacat
    23281 aaaactagat aatccaaata tgtcattgaa gtacatagaa aagataaatg aaatagataa
    23341 tagaaatgat agaaaagaag atgtcgatat tttggtgtta aaagcaaata atatgcttga
    23401 gattggaaaa tatgaagaat caagagatta ctttaaaaaa gcactaaaaa tacttgagag
    23461 tgaggataat aaaactggat tagcaaatgt gtatttaaca atttgtgatg tttacagaaa
    23521 aataggtgaa acagatagag tacttgaata ttcacaaaag gtatatgaca taaagaaaaa
    23581 tgatgaagat gaatacatga tgtcaggatt atttaagata atagaagcat atatagataa
    23641 agaagattat gatttagcta gaaaatattg taagatagcc ttagcttcat caataaaaaa
    23701 taaaaataaa ttcaatgaat ataaagcatt gagattctat gctaatatgt ataaaaatca
    23761 gaatgaagta actcttgcaa ttgaatatct gattaaatgc ataaaaatag tatcagattt
    23821 aggcaacaat agaatattgg ccaatttata tatagactta ggtcaattat attcaaatat
    23881 atcaaaagaa aaagagttag aatattatca aaaaggtgta tttatgtaca aaaatttaga
    23941 aataatgtag ggtaaataaa cctataaggg tatagaaaca atctataaca agcaagataa
    24001 tatctatgat aaaatataaa ttgtatgaat atttttaagg agatgaatca gacaatgagt
    24061 ataaaaatag atgcgagagg gcttgcttgt cctaaaccag ttataaatac aaaaagagaa
    24121 ttggataacc tagaagaagg tgttgtagtt acaatagttg ataatgaaac agctaaggag
    24181 aatatattaa aacttgctaa atctttaagt tgtgaagcaa atattgttga cgaaaaagag
    24241 aattttatat ctatagaaat tagaaaaggt aaaaatatac tagataaaca aaatgttgta
    24301 gataataaag aaagtgaatt agacaataca tgtatattta tatcttcaga taagatggga
    24361 cttggaaatg acgaattagg aaaagtgtta ataaaaggat ttatatatac attaacagaa
    24421 tctaaacctt atcctaagta tgtactttta gttaatggag gagtaaaatt aagtgctgaa
    24481 aatgaagaaa ccatagaaaa tctaaaaata cttgagaata tgggtgtaga agttttatct
    24541 tgtggtactt gtttagatta ttataattta aaagacaaat tacaagtagg tacagttaca
    24601 aatatgtata ctatagttga gacattaaag aatgcatcaa ataccatttc tatataatgt
    24661 atgtttgttt aataatataa aactaataat aaagattaac tcaaatagat agtcagaaag
    24721 gattaagtaa tgaatcaaat gtatatagtg tcatttaatt cgacacatca tgcgataaga
    24781 tcagaaaagt tatttggtga aaatagtcta aaagtaatgg ctcttccaac accaagagaa
    24841 ataacagcaa gttgtggaat atcaattaaa ttttcttttg aagatatgga aaagataaaa
    24901 actattttgg ttgaaaataa tgtagatata aaaggaattt attgtataag taaattagaa
    24961 aatggttcaa aagaagtaga gaagttagat taggaggcta attatttatg ccaatggatt
    25021 taaaaatagg tgatattgtt gaattaaaaa agcaacatgc atgtgggtgt aaagagtttg
    25081 aaatcataag aacgggaatg gatataaaga taaaatgtac taaatgttca aggctaataa
    25141 tgcttgatag agagacctta gaaaagagag taaaaaagct aataaaaaag tgataaaaat
    25201 ttaacactta aaaaatgtca aataccgtat attaatatcc aaaattttgg tataataaga
    25261 attgaagaaa acattttcat gggggtgctt taattatggc agttaaatat gcaaaaagaa
    25321 tgcaaggatt acaaggatca gaaatacgtg agcttttaaa acttactcaa caaccacaaa
    25381 taatatcttt tgctggtgga atgccggctc cagagttatt tccagttgag gaaatgaaaa
    25441 aagtatcagt tgcagtgctt gaagaaaatg gaagatcagc tatgcaatat actacaacag
    25501 aaggatatga accattaaga gaaaaaatag cagctagaat gaatgataaa aacaaaacta
    25561 atgtaaacaa agatgatata ttagttacaa gtggttctca acaaggtcta gattttgcag
    25621 gaaaagtatt tattgatgag ggtgatgtaa ttttatgtga aagtccatct tacataggag
    25681 ctataaacgc atttaaatct tatcaaccta aattcataga tgttccaaca gattctgatg
    25741 gaatgataat ggaagaatta gaaaagatac tagaaacaac tgatagaata aaaatgattt
    25801 acgtaatacc tgacttccaa aatccaacag gaagaacttg gccacttgaa agacgtaaaa
    25861 aattcatgga aatagtaaat aaatttgaaa taccagtaat agaagataat ccatatggag
    25921 atttaagatt tgaaggagag actttaccat ctttaaaatc aatggataca aaaggattag
    25981 taatattctt aggaactttc tctaaaatat tctgcccagg atacagatta ggatggactt
    26041 gtgcttctcc agaaatttta tctaaattta actttgcaaa acaaggtgca gacttacaag
    26101 catcaactat atctcaaatg gaagtaagca aatttatgga tatgtatgac ctagatgcac
    26161 atgttgacaa aataaaagca gtttatgtta aacgtagaga tgtaatgctt aagactatgg
    26221 aagaagagtt cccagaagga ttagtattta ctcatccaga aggtggatta tttacatggg
    26281 tagagcttcc aagtaattta aatgctaaag aattaatgcc aaaatgttta gagaagaatg
    26341 ttgcttatgt tgctggagga ggattcttcc ctaatggtgg tagagaaaat actttcagac
    26401 ttaactattc aaatatgcca gaagaaaaaa taatagaagg tataaaaaat atagcagctg
    26461 tattaaaaga agctatgggt gtagaagctt aattaaaaat aataacttat atataataca
    26521 agggatggtt gttgccatcc cttgtttgtt tgaaaagtat ataattcacc ttatactaaa
    26581 ctttgtccaa aatttaaatt gacagaaata agaataaatg ataaaatgtg taggtatgag
    26641 attttagtct catttctctg ctctaagagt gtagagccgt taagtccaaa gggaggtgaa
    26701 ttatagtgag aaattatgaa ttagtttatg tggtaaagcc aaattctgat gaagaagtaa
    26761 gagaggctat actaaacaaa gttaaggaag ttgttgcaac tgacggagaa atagttaaag
    26821 ttgatacttg gggaactaaa aaattagctt atccaatagc taagtttaca gaaggtttct
    26881 atgtattagt taacttcaaa tcagcagtag acgttcctaa agagatagac agaaacttaa
    26941 agataaatga aaacgtaata agacacatga tagttgttgc ttaataactg gttttattat
    27001 aaaaaatagg tggtgttttt atgaatcaag ttgtattagt gggaaggttg actagagacc
    27061 ctgaattaag atacatccca ggtacaggaa caccagtggc atcctttaca atagccattg
    27121 atagagacta tgtaaaaaaa gatggatcta aagaaactga ttttatacct gttgaagtaa
    27181 tggggaaatc tgctgaattt tgtgctaact acataactaa aggtagatta gtagcacttc
    27241 aaggatctat aagagtagac aattatcaga cacaatcagg tgaaaagaga acttttacaa
    27301 aagtaagtac tagatcagta caagctttgg atagtaaaaa taaatcggaa aattcatata
    27361 gagagagtgc tccagctttt gagccaagct ttgaacctca aggtttagac ccacaaggtt
    27421 ttcaagctat agatgatgac gatataccat tttaaaaagg agggaaaaaa gtcatgatga
    27481 ataagaaaag acgtaagaaa aaaagagttt gtcaattttg tgctgacaaa aacgctaaaa
    27541 tagattacaa aagtactcaa agattacaaa agtatataac tgaaagagga aagatattac
    27601 caagaagaat atctggaact tgtgctaagc atcaaagaga attaacagtt gctataaaaa
    27661 gagcgagaaa tatagcactt ttaccatata cattagacta aaaaaatgag ccttgtgctc
    27721 attttttatt atatgtattt atatatctaa atgaggcgag ctataaagct cgtttttttg
    27781 tttatatact taaatatatt attatttatg atataatatt atatattaag gagggttgta
    27841 aacgtgaaaa tagataaagg ttctgagata acgagaaaca taaagataat agaatggatg
    27901 aaaacagaaa tattaatgag tgtaagtgat ttgtttaatt tattgtttaa aggggtaaaa
    27961 ccattagatg aagcaattca agatacttta gcaaatataa taatgataac atatctctta
    28021 gcaaagagat taggaattag ttttagagat gtggattata aagtcaagga aaaaataaaa
    28081 attggtatag atgaggacca tagcgttgaa agatggtatg gagatttatc taatttaaaa
    28141 aaacatatgg ataataggga gtgatattaa attgaataat aaaattagac tatctaaggc
    28201 aatgtctata atagttttag ctataataat cgctttagta atagcctata ttccaatgct
    28261 aggaatattt agtatgttag cagctgttcc atatgtaatt ataggtgcta ttactgatag
    28321 aaagtattct cttatatcta ttttggttac attttgtgta ctgattctat ttgcagacat
    28381 ttcttatgca ttaaatatat gtataatgta ttctttacca ggtatagtaa taggaaagat
    28441 gctaaagaga agctttgaac aagaggacag taataaattc gaaccgatat atggtggaac
    28501 cataatattt gtactttcaa tgcttgtata tttttttgta ttaaagacat ttttaaatgt
    28561 gaatctatta gatgaagttt ctaagatgat atcagaaatt gtaaatattc aaaaaaatag
    28621 tttttctgca actgaactta aagtgtttga taaaatgaag ccagatgaga ttgtaagtta
    28681 ttttacaaat atgctaccaa tgatgttatt tttacaaggg ttgttatcag cgtttgtcac
    28741 atattatttg agtgtatttt ttataaaaag gattacaaag atgaatataa gatttcctaa
    28801 atttgctgat ttttatttac caggaaatgc tatactcaca actttcttac tttatttatt
    28861 agtcttattt attcaaatta ttggttcaaa attatataca gaattaataa tgactaattt
    28921 acagctagtt tttaatttaa tgtttgtaat tcaaggtata gcagtttgca tttatttctt
    28981 aaagaaatgg ataagacagg gaccaaataa aattatgttt ttttcaggga taattttatg
    29041 tttatttgga tttatgggaa tatcttttgt tggtatggta gatagtataa ttgactttag
    29101 aaaggtaaga agctataaat ccacttagga ggatagaaat gagtaacaaa caaaccttta
    29161 aattaaatat gccagaaata aatttatata taattgttat tggtatttct agtataattt
    29221 tgctttatta caacttgtat gtagggtgtt tatttttctg tatttttgta tatatggttt
    29281 ttcacaattg gagaactact aatattagac gtcatgaatg gacagagtat attcaaaatt
    29341 tatctttaga tatagatgaa acaactaaaa aagccataat aaatcttcca ataccattat
    29401 gtattttaga atttgatgga aatatatcct ggtataatgg aaagttttat gatatgattg
    29461 gtcaaaaaga tttacttgat aaaaatatag aggatatagt taaaaatctt aatttaagaa
    29521 aagtattaaa tgaaaataaa gagatgtaca cagaaataaa ctataaagaa aaagaatata
    29581 caataattta taatgttata aaaaatgatc aagaaaaaaa tcctaaatat ttgatgatat
    29641 tatactggat agacaagact gaatatttaa aagtaaaaca aaattatgat gatgaaaaaa
    29701 atgcaatgat gctaatacaa gtagatggat atgatgaagt attaaaaagt gcagcagaag
    29761 ataagagagc tttaattaat gttgaagtag agaaaatact ttctgcttta gaattaaatt
    29821 caaatggtgc attgagaaga acttcaaaag acaaattctt cttggtaatg cataaaaaag
    29881 agctaaaaaa attggaagct gagaagtttt ctattttaga tacaataaga catattgatt
    29941 atggaaataa tcttcctgtt actataagta taggaattgg tatagatgga gatacattaa
    30001 atgagaacct aaaacttgct acgggtgctc tcgatttagc acttggtaga ggtggtgatc
    30061 aggctgttgt aaaaacaaaa gataaatttg tgttttatgg aggaaaatct aaagctgtag
    30121 aaaagaaaac caaagtaaaa tccagattaa taggtcatgc tttaagagag gttatacaac
    30181 aaagtgacca agtttatata atggggcata aatatcctga tatggatgct atgggagcag
    30241 cagttggtgt ttatgatata tgtaaatcct gtaacaaaac tgcaaacata gttttacaat
    30301 ctgtaaatga atctatagaa atatttataa ataaaataaa tgaaaataat tactataaaa
    30361 agctgtttat tggaaaagag gaagctattg acaactgtac taaaaataca ttagttgtag
    30421 tagttgatac tcatagacca aattatactg aatgtgaaga actgttaaag ctatcagaga
    30481 aggtagtagt tatagaccat catagaagag gtgtggagtt tataaatgat gcagtgcttt
    30541 tattccatga gatatatgta tcctcaacgt gtgaaatggt tacagagtta gttcagtata
    30601 tggatgaaga tgtaacaata aataaactta cagctgaagg gcttttagca ggtataagtc
    30661 tagatactaa aaactttgca ttcaaaacag gtgttaggac ttttgaagct gcttcttacc
    30721 ttagaaaagt tggagcagat actatagagg taaagaaatt cttcaattct gatgtaaaag
    30781 atttcataat aaaagctgaa ataattcaaa gtactaaaat aattaataat agaatatgtt
    30841 tagcatattc aagtactgag atagatagta taaatgttat aatagctcaa actgctgatg
    30901 agttattaaa tatcaaagaa gtagaagctt cctttgtatt aggtgaaaaa gatgatacta
    30961 tatttataag tgcgaggtct ttagggcaaa taaatgtaca tgtacttatg gaaaaattag
    31021 gaggcggggg acacatagat atagctggag cacagttaaa aaatgtttct ctaaaagaag
    31081 cttataagat ggtaaataaa ataatagagg agtatttgga ggaggaagaa tagatgaaag
    31141 ttatattatt aaaagacgta aaaggaactg gtaaaaaggg agaaatgaaa gaagtaagtg
    31201 atggatatgc aagaaatttc ttatttccta aaaagatggc tgtgcaagca gatagtgtag
    31261 caataaaaga attaaatgaa aagaataaat ctaaagaaat aaaagcacaa aaagagtatg
    31321 aagaagcagt tttattaggt aagcaaatgg aagaaattaa tatagaaata tattcaaaag
    31381 caggagaagg tggaagatta tttggttcaa taacttctaa agaaattgct gagcaattaa
    31441 agaaacaaaa agacatagac gtagataaaa gaaaaatatt attagatgaa ccaataagat
    31501 ctttaggttc aactttcgta gaaataaaaa tacatcaaaa agtaactact aaaataagag
    31561 tggatgtaaa agaaaaacag taataactag aggtgattaa gatggaagat atgacgagaa
    31621 ttcctcctca tagtgtagaa tcagaacaat caatattggg ttctatactt ctagataaag
    31681 atgctataat aacggttaca gaaactataa agcctgatga tttctataaa gaggctcaca
    31741 aaataatata tgaatgtatg ataactttaa gtaataaagg tgagcctata gacttgataa
    31801 cattaacaga agaactaaga aaacaagggc atcttaatga tattggagga atcagttata
    31861 ttacaagtct ttctactata gtaccgacga cttcaaatgt aaaatactat gctgatatag
    31921 taaaagaaaa gtctgtactt agaaagctta taaaagcttc taatgaaata ataaatctag
    31981 gatatagtgg agcaactaag attgaagatg ttttagaaca agctgaaaag agtatatttg
    32041 atatatcaca ggagaaaact agtgatgatt ttaaatctat aaacttagtt ttgatggatg
    32101 cctatgatat gatagaaaaa ttatatacta acaagagtga tgtcactggt ataactactg
    32161 gttttaaaga tttaaacaaa aaaataaatg gtcttcaaag gactgattta atattaattg
    32221 cagctagacc agcaatgggt aaaactgcct tttctttaaa cttggttcaa aatgcagcct
    32281 taaaaggtga tgcctctgta gctgtattta gcttagagat gtctaaggaa caattagtac
    32341 aacgtatgtt atcttcacaa tctagtgtag aattaaaaaa aataaagact ggaacactta
    32401 atgataatga ttggcctaga attatagatg ctatggcagt attatcagat gccaaaatac
    32461 acatagatga tactcctggt ataaaaatat cagaactaag gtcaaagtgt agaaagttaa
    32521 aaatagaaaa aggactagat ttagtgctta ttgattatct tcaacttatg gaaggtgaag
    32581 gtaataatga aagcagacaa caagagatat ctaagatatc aaggtcacta aaaatattag
    32641 caaaagaatt aaactgtcct gtagttgcac tatctcagtt atcacgtgca ccagaacaaa
    32701 gagcagacca tagacctatg ttatctgatt taagagagtc tggagcaata gaacaggatg
    32761 cagatatagt tatgttctta tacagggacg aatattatca tgctgattct gagagtaaaa
    32821 atataggaga agtaataata gcaaaaaata gacacggtga aactggttct gtggaacttg
    32881 tttggcttgg tgaagttcaa agatttgggg ataagttgag agacctataa tctaacgaat
    32941 atccacagaa cgtcctttct ttctacagga aaaatgtgga tacatgtcac taaatatgtc
    33001 caaacattga aatttagcta aaaaactatc cacactatcc acagatagcc tgttgataag
    33061 tgtgaataag tagataaagc aagcttttgg cttgctttat ctgtgtataa atattaatta
    33121 ttaggaggaa taatatggaa aaaataagct taaaatatat atatcccaat ataattaaag
    33181 tacttgatga aataaactta tttagagtaa ttgacaataa tttaagagag tctattgttg
    33241 tttatgctaa taatgtggat aatcagtatc atataaacat gactaataca aactttggaa
    33301 acataattaa catatgtaaa ttagaaaaac ttttagatgt ggataaattt atggaaaaag
    33361 ttataaaata tgaaaaagaa ataatagaga aagaagagtt ttcaaaaata gaagaatata
    33421 tgttaaatat tggagaatac taaaaaagtt gaaaaaaata cgaatgtttg atagaataag
    33481 taagtaaagt gttcgagagt atataaaata tagaggtgaa ttacatgaaa acagttgcaa
    33541 ttgttggttc tcaatgggga gatgaaggta aaggtaaagt tatagattat cttgctactc
    33601 aagcagatgt agtagtaaga gggcaaggtg gaaataatgc aggacataca ttagttgtag
    33661 aaggtaaaaa atacgcatta catctaattc catctggagt tttaaatcca aatacagtga
    33721 atataatcgg aaatggaata gtgtttgacc ctaaaggatt tttagaagaa ttggaaatgt
    33781 ttaaaacgga taatataagt acagaaaata taaaaataag tgatagagca catgtcatat
    33841 tcccatatca taaagaacta gatgcattgt cagaagaagc cagaggcgat ttaaagatag
    33901 gtacaacaaa aaaaggtatt ggaccttgtt atatggataa aacagagaga tctggtatta
    33961 gaatatgtga cttaatggat aaagataaat ttgctataaa attaaaagct caaatagatg
    34021 ctaaaaataa aatagttaaa aatatatatg gaaaagaaga attatttgat tttgaaacta
    34081 tatataacga atatctagga tatgcagaac aaataagaaa atatgtggca gatacgtctg
    34141 ttattgtata tgatgcagta agagcaggta aaaaagtatt gtttgaagga gcacaaggga
    34201 ctttattaga tttagattta ggaacttatc catttgttac atcttctcat ccaacatctg
    34261 gtggatttgc aattggtgca ggtataggac caaatatgat aaaagacgtt gttggtatag
    34321 taaaagcata tactacaaga gttggagaag gaccgtttgt tacagagcaa ataaatgaaa
    34381 ctggagataa gataagagaa caaggacatg aatttggggt aactactggt agaccaagaa
    34441 gatgtgggtg gtttgatgca gttatagtta agtatgcagc tagagttaat ggattgacta
    34501 gtatttcatt tatgttactt gatgtattaa ctggatttga taagataaaa gtttgtacgt
    34561 cttataaaat gggcgataag ataataacgg atttcccagc ttcattagat gatttagcaa
    34621 aatgtgaacc tgtatatgaa gaattagatg gatggaatga agatataact caaatagata
    34681 actttgatga ccttccagaa aatgcaaaga aatatgtagc aaaaatagaa gaactagttg
    34741 gagtaagtgt agatatggtt tctgttggtc caaatagagc tcaaactata ataagaagaa
    34801 atatatttgc ttaatatttt atcaattaaa taatttaaaa tagttgaaga agagtatctc
    34861 aagataatgt ttatagttat tagagatgct cttttatatt tgacttacat tgatttttta
    34921 ttaagttaaa gctacttata catattctag ctgaaattaa agttgaattt atgtagatac
    34981 atataggttt tttaaaaaga attttgttga aaataaatgg ctaaagatga ttttaattat
    35041 attctaaaaa aagtaaaaaa aactattgac taaaaattgt atagacgcta taatataact
    35101 tgtgagtgaa agaaacgacg aaaaaagtcg aaaaaatgtg gtctattagc tcagtcggta
    35161 gagcacctga cttttaatca gggtgtcccg cgttcgagtc gcggatagat caccaaaata
    35221 tggctcgttg gtcaagaggt taagacaccg ccctttcacg gcggtaacag gggttcaatt
    35281 cccctacgag tcaccagtat gggactatag ctcagctggg agagcacctg ccttacaagc
    35341 agggggtcac aggttcgagc cctgttagtc ccaccaaatt ttgaattaga gataaatact
    35401 agattatatg ctggtgtggc tcaacggtag agcagctgac ttgtaatcag caggttgtag
    35461 gttcgattcc tatcaccagc tccacaaagt acataaattc aaattaatat aattttctat
    35521 gttacaggat acttttaagt atcttttttt aatttcattt ttattgtatt aaaaatgaaa
    35581 ttaaacatac aacaaaattt tgaattactt gcaatgtgat agttttaaat ataaaattta
    35641 gaatcattct agcatagtat tatgtaatat agcaaattta atttatagta tcaataaata
    35701 tagactacta agaatgtaaa tgatttgtta gattaagata cattagatat gtaaatagaa
    35761 aaataaaaaa tatgttttaa tttatgtggc ataagtttta aacagagaag caagtttgct
    35821 cttacctctc tatttaaaaa taaaaaaatg ttttttaagg gctattttat ttaaatcttt
    35881 cccatctcaa atcatctcca gtaaattcta aaatcctaaa cttatctaag attctagaga
    35941 gagttctttg agtgtaagta tttcctattt gagatggtgt taagttggtt gaaataatga
    36001 tttttttacc agcaacaagt cttgtattta caatattgaa gatttcccca ctagtaaaag
    36061 aattattaag ttcagttcca aggtcatcta ttattaataa atcacaatca aacaagtttt
    36121 tataattatc ttcacttatt tggttatttg tgtctcttct aaatttataa tcttcaagaa
    36181 tatctaaaat tctaaaagaa gtttgataga ttacaacatt tcctttatct aacaattcct
    36241 ttgcaataca attgcacata taggtctttc ctagtccagt agaaccataa aacagtagat
    36301 tttcactatt atccttctta aaatcatgaa caaaattttc gcagatactt aaattattta
    36361 gcatattttc tctaggagat atttccccat cagagccttt cttaggagaa aatatattca
    36421 agttaaaatt cgaaaaattt tcctgactta aaatcctatc taaattagac attttataag
    36481 cttcatttac aatttcttgt ttgaggcaag aacatttttc tccattaggt aaaaatcctt
    36541 tatctttaca agaaatacat tgaaacttta tttccaagta atcaagaggt atgttacttt
    36601 cagataaaag tttttctttc tttactttta aagactctat attctcttta gtcttcttta
    36661 caatttcatc tttagatttg ggatttaaaa gtactatctt agctaaactg agacctattt
    36721 tagaaatttc atcatctata gattttatct cagggattct attatatacc tcattttttc
    36781 tatgttctaa taagagttca ttatcatctc ttctcttagc atatttagca agtatttttc
    36841 ttattttatc ctcattcatt taatcaccta cttaaacttt tctttttgac tttttttaat
    36901 aatctcatcg agctcatcag aggtatactg agtgaatgtt tcgttgaaat tatgaaactt
    36961 agtttttttg taagtattgt tgttttgagt agtattaatc tgcttcttat taatattctc
    37021 cttaactata cgttcttcct ctttttgctt taaatcattt aaatttttta tatttttttc
    37081 attccaattt ttaattatac catttatata agatatactt gggtttgaaa tattcttaga
    37141 cttagaacat gcatttatta tcaaatctat atccatatta aatttatcaa accaggtgtc
    37201 cataagttct ttttctgatt ttgatggttg tcttgaaaat cccaattcat tgaaaatcgt
    37261 tttatataaa atatatcgtt cacttcttac caaaaagctt tcttcaacat cttttggcgt
    37321 atataaattt gaatcatacc aatttctaat aatgccttca atgtatttaa ctggctttga
    37381 tgtaccagtt ttatctttga cgtattcata tgcgcaaagt atcatatcgg ggctcatatt
    37441 gtatttattc attatatcca taatactcat cttttcactt gggtcgagat atcgcccaac
    37501 tattttattt atagaattaa acatttttct aatacttgga ttttcagaag tagatacaac
    37561 cttatctgta ttagatttta tagatgaatt attgcttaat atattctcaa tatagaaatt
    37621 ttttaaatct aaaaattcta ttgaataatt aaaattatca tattctccat tgtcatgtat
    37681 ttttataatc ttcttttctt cccagaattt ccaagcagat aacacatcag acaatggtat
    37741 atttaggttt ttggctattg aattattgtc gaacttagga ttagaagtaa tatcacatgc
    37801 ttgtctatat cctaaaagat aaacttttac gtataacccg tccgccattg gcataaatat
    37861 atctataaag atattgggaa tagtgatttc acctaaatct atttcattgc tttctttaaa
    37921 aaacatttta tcacctcaga ttaaattata tcatttttta tattatattt gtctgtaaac
    37981 aaataaaagg tcacaagatt agcatgtaaa tattttctaa aagtattcat tttgaaaagg
    38041 aggagatatt ttggttataa aattcaacca aaaagtaaga agaacattat cagtagcgat
    38101 tctatttatg gtattaataa taggttgctt tatagaaaag gatgacagtt tagaaacata
    38161 caaagaaaaa actaattcaa aaataaatca gtataatatg gatgttatat ttgatgatga
    38221 gactaagagg ttaatgtgca atcagaatgt tgactacata aataatacaa aatcaaacat
    38281 agataaaata tatttccata tatatccgaa tgctttttcc aagaaagatt ttgctccttt
    38341 tgaaaaaagt gaaatgacaa gggcttatcc taatggattt aacgaaggat atatagatat
    38401 aaagaatata ttaaataata atagtaaaat ggagtataaa attaaaggag acaaaaatga
    38461 catattagaa atagaattag ggaaagagtt aaaaccaaat gaaagaatgt cattagatat
    38521 aaaatataac gtaaaaatac caaattcaat tggaagattt ggatatggag aaaatactat
    38581 aaatgttact aattggtttc ctatagcttg tgtaaatgat gaaaggggat ggaatttaaa
    38641 gagttatgaa actattggcg acccatttta tagtgagact agcgattttc atgtgaaact
    38701 attgattcca aataaatata aaattgctca tacaggaaaa cttattgatg ataaacatga
    38761 taataagaaa atgctatatg aaatagaagc tgaaaacgtt agagattttg catttatact
    38821 aagtagtaaa tttgatgtaa acaaagtaga ttcaaaggga gtagctatca atacatataa
    38881 tttaaataaa aagctttcta aaaaagcaac tgaagtggca aaagattcaa taaatatatt
    38941 tagtgaatta tttggaaagt atccttacaa agaattttct gttgtagcaa gtgacttttt
    39001 tatagggggg atggaatacc ctatgcttgt tatgatagac caaagtttat ataatgaaaa
    39061 aaatgaattt ttactagaat atgttattgc ccatgagaca gcacatcaat ggtggtattc
    39121 tgctgttgga aatgatgaaa taagtgaacc ctggttagat gaagcactta ctgaatattc
    39181 tacaatagtt tattttgaac aaaagtatgg taaagatatg gcaaacaagc ttataaaaac
    39241 tatggagata cagacaaaaa gttatttgag tgaaaatata tttaagccag caaatgagta
    39301 taaaaattct actgaataca gcttgaatgt ttatacaaaa ggcgcaatag catttaatga
    39361 aataagaaaa gaagttggag ataaagtatt ctttgagact ttaaatgaat actataataa
    39421 atataagcat aaaaatgcta atggaagagc ttttgtggaa ctatggaata gtaaaggtgt
    39481 agatataaat aagattataa gtgaatgtaa gtaaatagtg aatgtaagta aattttgtgc
    39541 agagtattaa attttattta atactctgtt tttttggtat aatataattt ataaggcaga
    39601 aaaagaggtg aaggaatgct aaagtattgt tcagtaggga gtggtagtag tgggaattgc
    39661 cactatatag ggtataaaaa tactaatata ctagtagatg caggattaag tggcaagaga
    39721 ataactacag gtcttaaaga cattggtgta gatgcagata aattaaaagg aatatttata
    39781 acacatgagc atgttgacca tataaaagga gcaggtatat tatccagaaa gttcgatata
    39841 ccagtatttg ctaatataaa aacatggtgt tctatgaagg acaaattagg tgacatcaaa
    39901 gataaaaata tgaaagtatt tgaaaatgat aaaacctact cattaggtga tatcatagtg
    39961 agaccttttt caatagaaca tgatgcgtct gatccagttg gatataattt ttatacagag
    40021 agtgatgaaa aaatgtctat agctactgat ataggatgta taactcaaaa tataaaaaaa
    40081 catctatata aatctaaatt agtagtattg gaatcaaatt atgaccctaa tatgcttatg
    40141 atgggttcat atacatatgc tttaaagaaa agagtaatgt caagtactgg tcatttatcc
    40201 aatgaagatg cagctaattt ctgcgttgaa cttattaatg aaggtacaga atctatatta
    40261 ttagcacatt taagtaaaga aaataatttt cctgagttag catatgagac ttcaaaagga
    40321 gtattagcat caaatgatat agtagttgga caagatgtaa aactagatgt actatcacga
    40381 aatgatgttt ctaatgttta tgagatgaag tagaggtgaa taactttgct aaagagaaat
    40441 tttgaaaatg ttgaggataa taaaatttta tactctataa agcagggtat gattttagca
    40501 atacctgcta ttatgactgg ttcaacggct cttgtaattt tgaatcttcc aataaaggct
    40561 tatcaagaat atttatcagg cttgtttaat ggagaggtta caaatattct taactttata
    40621 aatgattcta ctttaggaat tatttcatta ataatattat taacaataag ctatagttat
    40681 ggaaaaatat atgggagcaa gtatacagta cttgttccta tagttgcaat gtgctctttt
    40741 ttagtatttt cacatggtaa tgagcttaat tcttatatag aaatatttaa gacaaagtgg
    40801 ttatttacat ctataattgt ttctatgaca tcttctgtat tgtttgtaaa acttacagaa
    40861 tcttttgtta caagtgtaaa gtttcataca gaaggggctg atgcagactt taatatggta
    40921 gtttcggcta tagttccttt cataatagta gtatttttgt tttctatttt tagagtgata
    40981 atgataagtc taattggcag ttcaaacttt caagatatat tttataattt attttcaaat
    41041 gtttttaata aaatgggaac aaaccttatg agtgctctgt tgtttatttt tttaatgcac
    41101 tttatgtggt tttttgggat tcatggtagt aatgtattag ataccgtcgc aaaaaattta
    41161 tttgaaaata gtatggcaat taacataaat ttagttaata ataatcaatt accaacagaa
    41221 atttttacta agaccttttt tgatacaatg gttttattgg gaggatgtgg ctctttacta
    41281 tgtttagtaa ttgcaatttt tcttagtgaa aaacgtataa atgtaagaaa gctagctaaa
    41341 attgcttcaa taccagctct ttttaatatc aatgagatgc tagttttcgg gataccaatt
    41401 gtttttaact atattatgtt tgtacctttt gtaattactc ctataatctt aaccattaca
    41461 agttatgtgg ctatgtctac aggtattgta ccatgtacag tttcagctgt agaatggacc
    41521 tcacctatat ttttaagtgg atatatgtct acaggttcaa taagaggtag tttgttgcaa
    41581 gtatttaatt tatgtgttgg tgttatgatt tatattccgt ttataaaaat gtcgcaaaat
    41641 agatatacat atatgtttaa gaataatata gaaaatttaa caaacttatt aaaaaggtat
    41701 gaattaacag gtgaacagcc taatcttcta aaccataatg gtaaaatagg ttcaatttca
    41761 aaaatgttgg catcagactt gaagtttgct atgaaaagag gagaaataga acttttttat
    41821 caaccacaag tcaattataa tggaaatgtt gtgggagcag agggattact tagatggaaa
    41881 cattcaatag gagggttcat ttatccacca ctagttataa ttttggctaa agaagaaggg
    41941 ttcttagatg agcttggaga gtatattatt aataaagcat gtattgattt aaagaagagt
    42001 gaaaagctat tgagaaatcc tgttaagttc tctgtaaata tatctccaga ccaacttgat
    42061 gaccctaagt taccggagca aataaaaaat atcattagta gtaatgatat aaatcctaat
    42121 atgttaggta tagaaataac agagcaaatt gcattatcag gttcacaaat aattattgaa
    42181 cgtatcaatg cgatacataa cttgggaata aagttaatta tggatgattt tgggatgggg
    42241 catagctctc ttatatatct tcaaaataat aactttgata tagttaaatt ggatggttca
    42301 ttagttaaag agatacttac aaataaaaga agtagtgaaa ttataatgac tattgtaaat
    42361 ctttcaaaaa atttagattt tgatatagtt gttgagtatg ttgaaaatat agaccaaagg
    42421 gataagttat atggtttagg atgtgaaata tatcaaggat attattatag caaagcaata
    42481 ccttttgaag agtttgtaga atatgcaaat caagaaaaag agatataatt ttataagagc
    42541 ttgtaaattc tagtaatgca tagatttgta ataaaattaa gtttaggaga aatatatgaa
    42601 tataagtata gtagtagtag gtaaaattaa agaaaaatat ttaaaattag gtattgatga
    42661 gttcaaaaaa aggctttcta agtattgtaa gttagaaatt atagagttgg atgatgaaaa
    42721 agctccagag aatttgagtg ttaaggaaat ggaaattata aaagacaaag aaggaaagaa
    42781 aattttaggg aagataaagc ataatagtta tgttatagtc ttggctattg atggtaaggg
    42841 tttatcctct gaagaacttg ctaagactat gagtgattta gcagttagag gtaatagtag
    42901 tctctgtttt ataataggag gttctttagg gctttctgat gaggttttag gaagggctga
    42961 ttataggtta tccttttcta agatgacttt tcctcatcag atgatgagat tgatattgtt
    43021 agaacagatt tatagggctt ataggataaa taatggagaa ccgtatcata aataaatgat
    43081 acggtttttg ctaatttgac acttaataat attaaaataa caaaaaagta ttgaaagtat
    43141 attgcattta aggtagaatt aaataaaata ttataacatt ataatgttta tatagttgtt
    43201 aataaaaatt tggaggaaaa tatatgataa aaataatgtt ggcctgttca gcaggaatgt
    43261 ctacaagttt attagttact aaaatggaaa gtgcagctaa agaaaatggt atagagtcgc
    43321 aaatttgggc aataccagaa tcaactatac aaaatgaaat agaaaaatgt gatgtattat
    43381 tactaggacc acaagttaga tatgtattac ctaaggctca agaaattgct aagccatata
    43441 atataccagt agaggttata aatatgatgc actatggaac agttaatgga gaagctgttt
    43501 taaatagagc gatagaactt aacaacacta agtaatataa attataatgt atttttaata
    43561 tataaattgg gagaatcaaa ggatggatga aaagattata gaaatatctt ttaatataat
    43621 aggttatgca ggtgaaggta aagggttagc ttttgaagca ataaaagagg ctaagaatgg
    43681 aaatatagaa aaagcaaaag aattattaaa agagtctaag gaagttataa ataaagcaca
    43741 tagatatcaa acagaattaa tacaaaacga agctagtgga aacaggactg aaataagtgt
    43801 catacttata catgcacagg accacctaat gaatggtatg aattttcaac agttagctga
    43861 agaaatcgtt gatttacatt tgaaattaca agataaataa aaattaaaga gaataaatta
    43921 agagagttct taaaagatac attatgtgta gcacactaat tgttagatta ttgattcaat
    43981 atttagtgag taatacatta ggattatttt gaggactctt tttatattta gtttaaagtt
    44041 aataaatatt tattattaat aatataagat tatatgttat aatattataa catataattt
    44101 aagaggtgaa tcatgttaag aaatcaacca ttatatatac aagttgtaga tatattaaag
    44161 caaaaaatat taaataacga atataaaatt gatgaaaata ttccaacaga aagagaatta
    44221 gagcaagaat ttggagtcag taaaattact attagaaaag ctattgagat attagaatca
    44281 gaagggtatg tagagaaaaa aagtggaaaa gggacaaaag taataagtaa tagtcttttt
    44341 aataagctat caaaagcaga gtctttttca agtatacttt tatctaaagg tcataagtta
    44401 tctaaaaaga ctatttcaat tgaaaaaata ataaatgata caaattcaca agtatttagt
    44461 atatttggag aaaaatgtag caagattact agaatgtatt acttagatag taagccatat
    44521 atatttttta ctcattatat tccttttgat gaaaatctaa ctgttgaaag ttttaataat
    44581 gaaagttctt tatatatgta cttatatagg agaaatataa atataactag atttgaagat
    44641 gaatttttta tagaagaatc agaactagag gtatcaaagg ctctaaatct agctaaagac
    44701 atattattag gaagaaaaag aaagacctat gatgagaatg ataatatagt tgagctatca
    44761 tttgcaagat ataatacaga agtacaaaac tacgtaatta aatacgaaat ataataagaa
    44821 tcctgagttt attgaaatga taatatgctc aggatttttg tttagttgta aaaacgaaaa
    44881 atattataac attataatgt ttacaaccaa acattataat gttataatat ttttaataaa
    44941 taagtgatat tatcaattac ataaaaatta gtttatgtta tgaaatcgtt attgcattat
    45001 aaataaagga taatatcaat aatacagggg gataactatg gaaaaattcg caagtttttt
    45061 agagcgcaag attatgcctg tagcagcgaa gataagtaat cagagacata tgagagctgt
    45121 aagagcaggt ataatagcaa cattaccact aacaatagtg gggtcatttt tcacaatatt
    45181 attaaacata cctattgatg cttatatgga ttttatagct ccatttaaag caacattaga
    45241 tataccattt agatatactg taggattttt atctctatat gctacttttg ggatagcatc
    45301 atcacttgct aaaagctacg atttagatac aacaggtgtt ggtatgcttg cagttatggc
    45361 atttttagtt tctacaatag ttccaactca agtagttgaa ccagtaggtg gagttataga
    45421 agctggtaga tggatggata tggcaaaact taattcgcca tcattatttg gagctatcgt
    45481 aacatcatta attacagttg aaatatatcg ttttatgaag gaaaagaata ttacaattaa
    45541 gatgccagca ggagttccag tagaggtttc aaattcattt actgcattat taccgacagc
    45601 agtaatttta atgttattct gggtaataag acatatttta ggatttgata taagtgttgc
    45661 actaagcgct atgttatcac ctttaaaagg attcttatca ggaaatagtt taggtggtgg
    45721 gctactgaca gtattcctaa taactttatt ctgggtactt gggatacatg gtccagctat
    45781 aatggggcca gtaataagac ctatatggga catgtctata gcagaaaata taactgcatt
    45841 cacagaagga gtatcagctc atgcaatgcc aaacatattt acagaacaat ttttacagtg
    45901 gtatgtatgg atagggggag caggggctac tcttgcatta gttgtaatgt ttatgttttc
    45961 taagtcacaa tatctaaaaa gtttaggtag attaggattt ttaccagggt tattcaacat
    46021 aaatgaacct ataatatttg gagcaccaat agttatgaat ccaatactag ggataccatt
    46081 tataattgga cctataataa tgacaattct atcatatgtt cttactatta caggggttat
    46141 acctatgatg gtagcaagat taccatttgc aatgccagca ccaatagcag cagtaatgag
    46201 tactaactgg agtatactag caggtatctt agtattaatc aactttgtga tatcatttgc
    46261 tgtttattat ccattcttca aagtttttga aaaacaacaa ttacaaagag aacaagaaga
    46321 aatgagtgaa aatttataaa aatacataga tatagaggtt ttgtactata taaaatatag
    46381 aaaaagctta taagttatta agggtgaagg taggaggaat gacctttgcc tttaatttta
    46441 tatattttaa ataatgtaat ttgaggagga gaaagatgga aagaagatta ggaatatcag
    46501 tatatccaga gcatagtaac atggaagatg ataaggcata tataaaaatg gtttcaaaat
    46561 acggatttaa aagaatattt atgtgcatgc tttcaattac aagacctaag gaagaagtta
    46621 aaaaacattt taaagagatt ataacctatg caagagaatt aggatttgaa gtaatccttg
    46681 atatagctcc aaacatattt gatatattac aaatatctta tgatgattta acattcttta
    46741 gtgagttaaa tgctagtgga ataaggcttg actatggata tgatggaagt aaagaagcta
    46801 tgatttcttt caatccatat gatttaaaag tggaactaaa tatgagtaat gatgtagcat
    46861 acttggataa tataattact tacaagccaa atgtaaataa tatatatggt tgtcacaatt
    46921 tttatcctca aaggggtaca ggcttgccat atgatttttt tgtaagatgc tcaaatagat
    46981 ttaaaaagta tggaataaga actgctgctt ttgtcaattc aaaatacgca acaatcggac
    47041 cttggaatat aaatgatgga ctgtgtacgc ttgaaatgca tagaaactta gatattgcta
    47101 cagcaactaa gcacttattc gcaacaggat tgattgatga tgtaattgta ggaaatgcat
    47161 atgcaagtga agatgaatta aaaagaatgt ctgaagtaaa tgaataccaa gttgtattta
    47221 atgtagtagc taaagaaaaa acttctgaag tagaaaagat aataatggta aatgagcaac
    47281 actttagaag aggagatata actaatcagg tagcaagatc tacagaagta agaaaaaaat
    47341 atgctaaaga aaatttccct aaacatgata ataagaacat ctttaaaaga ggagatgtag
    47401 tgattggtaa tgatgacttt ggaaagtata aaggggaatt acagttgata cttcaagatt
    47461 gtacagatga aagaaaaaat aaggtgggta gaataaagga agacgaaata atacttttag
    47521 actttattga accttggagt aaatttaaga ttattttaga gggagagtaa agtgaagata
    47581 gacatattaa taaaatgtgg aaaaacagta gataaaagta taattgatat agctatatta
    47641 gacgataaaa taatagaagt aaaaaatcat atagatgatt ctaagtatca ggcaaaacaa
    47701 attataaact tagatggaga gaaatttatt agtgcgggtt ggatagatat acatacacac
    47761 tgctacgaaa gactagacct ttataaggat tatccagatg aggttggaat aaaaagtgga
    47821 gttactactg tagtagatgc aggaacaaca ggagctttag atataggtga attttatgaa
    47881 gatactaaaa agtataagac caatgtatat gctttaataa atattgctaa gcagggaata
    47941 acatctcaag atgaactttc aaatatgatg aatattgatg aatatgaact taaaaatgca
    48001 gttagaaaat acaaagattt tgttgttgga atcaaagcta gaatgagtaa aagtgttgta
    48061 atttcaaatg atgttgaacc tttaaaagta gctaaaagaa taaaaaatga acttaatcta
    48121 cctatgatgg ttcactttgg gagttctcct ccaacgatag aggatatatt tgattatatg
    48181 gaaaaaggag atatattaac ccatatatat aatggaaagc caaatggaat acttagaggc
    48241 aatgaggtta aaaaggaaat catagaagct aaagaaagag gtataattct tgatgtaggt
    48301 catggaaccg aaagttttag tatggatatt gcaatgaaat ctaaggatgc aggaattttt
    48361 ccagacacta taagtacaga tatatatatt aaaaatagaa taaatggtcc agtgtataat
    48421 ctatctacaa caatggaaaa gtttatatat atgggctatt cacttgaaga tataatagat
    48481 aaagttacta aaaatgcagc agatgcaata agtttaaaaa ataaaggttt aataaaagaa
    48541 ggatatgatg ctgatttaac tatatttgat gtggttaatg aagaaaaaga acttcaagat
    48601 tctttgaata agtcagtaat cacaagtacg tctataaagc caagagcagt agtagtcaat
    48661 ggagaatatt taaatatagg caactctata gaagagaatg agtagaaggg agaaactatt
    48721 gtatgagtag tatatttgaa aaatactctg taagacgtgt tataaatgct tcaggcaaaa
    48781 tgactatact aggagtatca acagtagata aggaagttat tgaagcaata ggtgaagggt
    48841 gtaaaaattt ccttataata gaagaacttg taaataaaac aggtgaatat atagcaaatt
    48901 tattagggtg tgaaagtgca ctaatagttt ctagtgcatc tgctgggatt gcacagagtg
    48961 tgggagcaat gatagcaaaa gatgatatga gtctagttta caatgtaaat aatcctaaga
    49021 aacaagttaa aagggaaata ataattccaa aggggcataa tgttgattat ggagttccag
    49081 tagaagtaat gataagactt ggaggaggag aagttgttga ggccggctat gctaatatgt
    49141 gtaacaagga acatataata agagagataa atgaaaatac agcagcaatt atgtatatta
    49201 aatctcatca ttgtgttcaa aagagtatgc taagtgtatt agatgcagta caagttgcta
    49261 aagaatataa tttacctttg atagtagatg cagcagcaga agaagattta aatgtatatt
    49321 atgaattggg agcagaccta gtgatataca gtggtgctaa ggctatagaa ggtcctagct
    49381 cggggttagt aataggtaag aaaaaataca tagataatgt aaagcttcaa tcaaagggta
    49441 taggaagagc tatgaaaata ggaaaagaaa atattgtagg tctaacatgt gcaattgaga
    49501 gatatataag taatgataaa gtaactttaa aagaaatgga ggacaagcta aatccattta
    49561 tagacaagat aaatactata aagggagtga gtgctagtat aactagagat tcagcaggta
    49621 gagaaatact tcgtggagaa attaacttta atgaagatat aataaataag tcaacacaac
    49681 aaattataaa tgaacttaga agtggagaga tagctatata cactagagac tataaggcta
    49741 atgaaggcaa gattgagatt gatataagaa gtgtaactgg aagtgattta gataatattt
    49801 ataataggat aaaaaatata gtagaaagtt agagtgtaga gtaagtgaca ttataatgat
    49861 ttaataaatt gaattttacc aatggagggg ataaatatga attgtatacc acaatattat
    49921 aaaaatagag tttgtttaaa tgttcttgca ggaagcataa aaaatgctaa agaagtatat
    49981 gatgcctgtg atggacatgt actagtagga gtactatcaa agaattatag cactgtagaa
    50041 gcagctattg aagatatgaa aaaatactca aaggaaatag ataatgcaat atctgtgggt
    50101 cttggagctg gagacccaaa tcagtcaaat atggtatctc agatttctaa agaacttcag
    50161 ccacaacata taaatcaagt gtttacaggt gtcgctacat ctagagcatt gcttggtcaa
    50221 aatgatagta taataaatgg attagtttct ccaactggta aaccaggatt tgtaaaaata
    50281 tccacaggtc cattaagttc aaaagaaaaa gatggtatag ttcctataga aacagcaata
    50341 gctatgatga aagatatggg aggaagctca attaagtttt ttccaatggg agggcttaaa
    50401 acaaaggatg aatatataga agttgcaaaa gcttgtgcaa agcataattt ttatttagaa
    50461 ccaacagggg gtatagactt agataacttc aaagagatag ttcaaattgc acttgatgct
    50521 ggtgtagaaa aagttatacc tcatgtttat acatcaataa ttgataaaga aacaggtgat
    50581 acgaaagtgg aagatatagg agtactttat aaaataatga aagaattatt gggatagatt
    50641 tatgaatata ttagcttttg gtgaaataat gatgagactt tcagtacctg actacaagtt
    50701 cttaacacaa accaatgaac taaattatat aattacaggc acgggattaa atatattatc
    50761 tggacttaaa aactttggat acaataccta tatgttgaca aagttgccta ataataatgt
    50821 aggtaaagca tcaagtgcaa atataagaaa gcttggagta aaagatgact ttatcacata
    50881 tggagggaat catataggag tgtattttct tgaaaatggg tatggagaaa gaccttctga
    50941 agtgacttat ttgaataggt taaatagttc attttgtgag agtaaattac aagattatga
    51001 tattgataaa tgtttagaag aaatggatat agtccatatt tgtggtattg ctatgcaact
    51061 tacagattca gttaaaaaga ttgctttaga actagctaga aaatcttatg aaaaggggat
    51121 aaaagtattc tttgatttta attttagaca atcattaaat ccagaaagga cttatgatga
    51181 tttagtaaat gattataaga aaatactacc atattgtcat gggctgtttg ctagttatag
    51241 agatataaat aagatactta atatagatag tctaaatata aaatctagtt cagaattgat
    51301 taaaaaagag tatgatatag atattgtagc tggaactatg agaagatttg atgatatgaa
    51361 taatagattt ataaagggat atatatcaaa tgatgatggt tattttgaaa gtagggagta
    51421 taagttagaa atatatgaca gagttggtgc tggggatgga tatgtggcag gaattatata
    51481 ttcatatttg gaaaatatga agagtgaaga tatagttgag tttggggttg ctagttcggt
    51541 gttagctcat acaacttatg gggataattc tttagtaagc acaggtcatg tacttagact
    51601 gatgaacaat gaaaaaatag atatattgag ataatacatt tgtatataat aaaattttta
    51661 tcttttttag acacactttt gacttgctta tatttgttaa acagagttat ttttaacgat
    51721 tataaatatt aaattaaaag ttagataata aaatagtaga aaatagcatt aaccatataa
    51781 aaatggttaa tgctattttt atagttaata ttgattaatt ttatattata gaaatgaact
    51841 tttctaatgc tggatttgta ttttttttat tccatacaac cacaacttca attctatttt
    51901 ctacttcttt tataggtact attgcaatat cacgttctat cattgtatct gtaacacaaa
    51961 atgaaggtaa aatggatatt cccatgttag aagataccaa tgattcaata gtattaagat
    52021 tatttgaacg ttgtaccaca ttaggtacaa accccgcttc acgatatcta ttttgtatca
    52081 ttgacttttg tccatactca tcccctgttt cttttacaaa aataaattta tcatgttgta
    52141 attcaattaa atcgatagaa tcctcattgt aaagtggatg accaggaggt aaaagagcta
    52201 ccaaagaata tctttctaca gttttatatt caaaatcttt taatggttga ttttctggat
    52261 taataacaaa agcgaggtcc atagaattat tttttactaa gtttaaaagt tcggttattt
    52321 cattagtaca aatatccata gatatattag ggtatgtatt agaaaattct tttaagtatt
    52381 gttgaagttt gtttttttca tatcctatta aggttccaat gctcaaagtt ccaaaaaaac
    52441 catgtgcaaa ttgttgtgtt tttgcaatag catcattgat attttttatt atagtacgtg
    52501 cctcagataa aaaaactttt ccggctggtg ttagttcaac atgacgttta cttcttgtaa
    52561 atagagttac atttatttgt tcttctaaag cttttatttg ctgtgtaaca gcagtttgag
    52621 aaatataaaa tttttctgct gttttggtaa aacttagatt ttctgctaca gatataaaat
    52681 attctaattg tttagtattc aaataatcac ccctatttaa acttgattgt ttgtaaacct
    52741 tatttatcta taattaaagc ttattatagt ataagaaaat ttacttttaa agaatattag
    52801 aaaatatttg tataaaaata tatgttttaa gtatatcttt acattagatt ttataaattt
    52861 attaataaga ataacttata tattccaatg aaaacgatat tggatttaag taagtttgtc
    52921 tcataaaatg aaagtatatc aattgataat actttaaata agaagggaga aaaaatggaa
    52981 gataaatttt atgcaaaagg caacggaaat aacggatata ttaaaaatct tgaagtttgt
    53041 tcctttaata acttagatgg aacttgtgga atgtttcaaa tggctctgta caaaagagat
    53101 gaaaaatact atttatatgg atgctgtttt ggaggaaata aaaaaaatgg agtaatgatt
    53161 agcgatatta cagaccctta taatccacaa tttataaaac attttcaaat gttagaccct
    53221 aaagagtatc ctacaacaac aactccaaaa attcaaatag cagatgattt aatgatagta
    53281 gcaatgagtt gtggaagtgg accaggagca cttgttgacc aagctaaatt agcaaatatt
    53341 aagtgtgaag caggaattag aatatacagt ttaaaagaag accctttaaa tcctaagttt
    53401 ttaggatatt gggattgtgg cttaaagcat gtaatgggtg ttcatagatt catgtacaat
    53461 ggtggaagat atgtacattt atcaagtgat tgtgttggct ttgaaggtct gatttatagg
    53521 gtcatagata taataaatcc taataatcca gtggaaatag gtaaatggtg gagaccagac
    53581 caatatgcag atggatatcc aaatagaact tttgatgcag gagcacctca ttgcccagaa
    53641 tttatggata aaggatggct tcatggacct ccatttgtaa gagacggaaa agcatattgt
    53701 ggttatggag gagctggttt agttgtatta gatgttgaag atttaacaag accaagatgc
    53761 ttaggtgaat tgccatttat gcctgcattt tctagtagac ttgcaggtgc aagaactcat
    53821 acagcattac cattgccagg aagagattta gtcgttgttc aaaatgaggg agaaagattc
    53881 cagttcttta aaccagataa cattacagat gttcaagcta tgaataatat acatatggtt
    53941 gatgttagtg acccaacaaa accaacatta attgctcaat tcccatatcc tgaagttcca
    54001 aaagatttcc cttatcctaa ctttaatgtt gcaggattag gaaaagcagg gccatttggt
    54061 ccacataatc ttcatgaacc aatggataat aagccatggt tagagcaaag aggagataga
    54121 gtatattgct gttatttcca tgcagggcta agggtttatg atgtatcaga cccatattat
    54181 atcaaagagc tagcatattt tataccacca aatccaaata aaacaccaga agaatcttat
    54241 ttcccaggat tcccaggacc acgcttggca gtaacagaag atcttatcgt tgatgataga
    54301 ggctacatca tcatagatgc tttagatgat ggattctata tattaaaaat gaaagaagat
    54361 taaataaata ctaaagaggg agagttgaaa atgaaaatac taggtatttc ttttggaaca
    54421 aaaaatggaa ataatgacac aatgtgtaaa gttgcactta aaggtgccaa agaagcaggg
    54481 gctgaagtag aatttattca aatgtcatct attaacatta aacattgtac tggatgttgt
    54541 gcgtgcgtta aaactttatt atccgggaaa ggtagtatgt gtgtattaaa agatgatttt
    54601 gaatggttgc ttgacaaaat gaaagatgca gatggtatag tagtttcaga ccctattttt
    54661 gaagaagggg catcaggatt attccatact ataatggacc gttttggacc cagagcagat
    54721 agaggaaata acattatagg aacaaaagtt gcagaagcta taggaggaaa aattccagac
    54781 tcaagaatgc ttaatgataa agtaatatca tttatgggaa taggaggttc tgactggggt
    54841 acaagagttc aatgtgaaca tgctatgcta gctctcatac caatgtggaa agttatagat
    54901 aatgcttggt tcccatgggc aaaggaatta gtaatggatg atacaaggct tgttcaagtt
    54961 catcaaattg gtttaaatat agttgaagca gcaaaagatt ttgaaaaagc ttcatatcaa
    55021 ggagaagaag gtgtatgtcc acattgccac aatagattat tctaccttga gtcaggtact
    55081 aaaaaggcaa tttgtgctct atgtggtata gttggagagt tagatactat aagtggaaaa
    55141 actatattta gttttccaga agagcaatta ggtcatgctc atgatacttt accaggtaag
    55201 tttatacatg gtgatgatat taagaaaatg gaaggtcatt atgcagaagt tcgtaaaaca
    55261 caagaattta aagaaagaaa aacatcttat aattttatag agccattaac taaagaaaag
    55321 caagctggag tttcattagg ataataatta aaatactaag actctttttt aagggatgtt
    55381 agatgttata ggaggcttat tgtatgaatg aaaagccaag ttttaaacaa attgaaatta
    55441 ttttagcagt tatttttttg gtacttacat attacaagat tggattaact ccagcattta
    55501 ttgttatatt tataccatta tacttaagtt attttgcagt atctatgatt gtaaatcaaa
    55561 tagcaattaa gctattggag ataaggcaag ctagggaagc tactaattac aatataggaa
    55621 atggatagaa agaaaaaaga aatattaaca ctgtctatgg ggattgcttt actacctcct
    55681 ttatgggcag tattagcacc ttatatagga ataaaaactg gagctgtagc actaatctgt
    55741 gcaggattat atgtaactaa tggtaataaa caaaaagatg gattaaaaat aatgtttggt
    55801 ttttggtgtg gagatttatg ggcagtgtta gccattttaa ttatgggata tatgaatttc
    55861 aatcaaaacc tagaattatt tttaacactt agtatattag gtttttttgc agttgtaatt
    55921 gcatccttat ttgaaaaaat aattttccta ccatcatggc tttgtggttg ggctataggt
    55981 ctaactatta tgacaggtga aaatattatt aatttacaag atatttgtat tcaaattgct
    56041 gtagctatgg cagtaggagt atggtacgtg ggtgcaggtg tagatttgtt tacaatatat
    56101 attttgagta aagataagaa aaagggaaat tgaaagcatg tgaatatact ttgattatag
    56161 gagaatggct atgggttttg aatttaaaat aatgagaagt ttaatatatg taggacttgc
    56221 caaggaagaa tatagaccta agttaatgga ctggttatat cgtcaccata ttccagatag
    56281 tattagcact tttggaccat attgtactaa atatgccttt tatcaagcat atcctacacc
    56341 aaatgaaggt gagcgttttg gtgcacgtaa gatgcaacta acagaacatt attggcttgt
    56401 agatgaacat atgcctgaga tggcaaatag aattatgaca gaatatatgc ctatggatgt
    56461 tctacgttgg caagggtgta taccagatgt agaaaataaa agggttcatg aaaatgcaga
    56521 aagtggagat gcaggacgtg cagtaggtgg agataatgga tgcccaccat ttatatttgc
    56581 ttttgttcca ataaactggg aagaagactt tagaggaaaa ggacgtactg tacaagatgg
    56641 accaaactat cgttggcaat ttatgattaa gtatccagat ggtatctcta aagaagaagg
    56701 agaaaaatgg ttctatgatg aggtagtgcc atactttaca aactgttgct atgttaatcg
    56761 ttttgtcagt agtaaaataa tgattaatta tggagcaact gcttttgacc gtgtatcaga
    56821 actatggttt gaaggggaag aggaatggta taaagctgtg gttgaagaga caaagtcgtt
    56881 tattaaaaaa ccagaatggg cacaagaaga ggagttccca tatttaaaac cacaattcaa
    56941 tatcgcatca gtattcttag gtgatatagc aactatggat gcatactcac agtatcgtgg
    57001 atatatacca atgagataaa aattatagtt tgtgataagt agaggaagta cttactctta
    57061 ctgctagaag aagacttatt gtacacttaa aagaatgaaa tagataagtg tcaatttatt
    57121 atatgagaga aactttaact ttcttggttt aaaatattaa agtcataagg aacatttatt
    57181 attaaatact ttatttatat gaattaattg aagaaaaagg atattaaact acaagttaaa
    57241 aaataaattt aatatattag aattataaat aaggcacatt aattttattt taaattaatg
    57301 tgccttttat ttatggtaat ttatgcataa ttagtatcgc tttgattctt taaataaatt
    57361 ttttttggtt tctttacata aggaaccctt ttttatttat aaaatctata agtgtaatat
    57421 agtagctatt ttagataagt ttaatttttg tctcaatcta aaacaaaagt tatacttaaa
    57481 tgaaattgaa aacgataaaa ttctcaaata aaataagtat tgtggaggtg atgaaagaat
    57541 ccaatgataa tatgaagact aatcttaata agaaagtagg ggttaatggt agtggacaaa
    57601 gaaattgtat caattctcca gattttatta aatagcacat tgattacaac taatggatta
    57661 caagaggagt ctaattcatc aaagagacaa attacttata gaataaataa aatcaatgac
    57721 atgcttaaag taaaaaatgt accacttata tgtttaagag ctgataaaga tattatcgtt
    57781 agaaaagaaa caaaaaaagc aattaaagaa attttagaaa aaaactattc taagaatact
    57841 tattatttta gtaagacaga aagactttta tatatgtatt taatgctttt tattaacatg
    57901 gaggatattt caattaatca ttttataaat tctataaaag taagtaggtc tactgtaaat
    57961 cttgatttca aggatttgat accagagctt gaaaagaaga acattaaagt taagaacaat
    58021 cgtataaatg gatattacat agttggcaat gagatggaaa ttaggagagt gcttattaaa
    58081 aacattatag aaactttgtc taatgagaaa agttcaagag tatttgatat ttttattaaa
    58141 gaatataatt tagatagttt tgaagaggct aaaaaaatta tattaaagct aataaaaaaa
    58201 tatgaaatta cttttgttga agatagatta atagaattta tatatatatt tatctttcta
    58261 aaagcaagga tgtattcaac tagaatagtt actcaggaaa atgtagatat tcctaatatt
    58321 tcagttatga gttcaatgaa ggagtataaa tttgctaagg aattattaga agtatatggg
    58381 agtgaggata agataaagcc ttataatata atgtatatta gtgcttggat tttaggtata
    58441 tcggtaggaa attatgaaga agatacagaa gataaggatg taatttcaaa aattgtaaat
    58501 aaaatgatga caaagtttgg atatttaagc ggagtttact atataagcca agaaaaaata
    58561 tttaaacaat tatattctca ttttagacct gcctactata gacttttatt taaattacct
    58621 atctataatc cgatttgtga aaaagtaaaa gaagaatata aattagttta tagaattgta
    58681 agtaatgcaa tgaaagagtt ttgcggatta tttggagaag aaattcagga agaagagtta
    58741 gcatatctta caatgcactt tgcaacactt ttttcaaata aaaaagaatt tgatggtttt
    58801 agaaaaagaa tagcattaat agtttgttca aatggcgttg gtagttcagc tattttacat
    58861 gcagaactta caaacttatt tcctgactta cactttatac caatgtcgga atcagttgga
    58921 ttagaaaata taagtgaacc tgtagatatc gtatttacta ctaattataa cgcagagggt
    58981 ttaaaaacag atgtaccagt aattaaagta agccctgtta tgaccacaaa agaaaaatat
    59041 aagattacac gtgaagtgta tattcaatta ggtgatattt atttaagaca acctaaaata
    59101 gatgaggtta tggatataat aaaaaaatat gcaaatgtaa cctctgaaag tattctttca
    59161 agtgagctat tagcttattt tacacagata gagaatttta catctaagga aggagagggg
    59221 cctatgctaa gtgagattac taatgaaaca ctagtaaaaa taaaggtaaa agctaagaat
    59281 tgggaggaag ctattagaca gtcagcttct gtattagttg aaaataacaa agtaactgaa
    59341 gggtatgtag atgctatgat taactcagca aaagcatctg gaccatacat agtaattaca
    59401 aaacatgttg cacttccaca tgctagacca gaagctgggt caaaagagat tgcaataggc
    59461 attgctactt tagatagtcc tattgagttt gggaataacg aaaacgaccc tgtaaaatat
    59521 gtattttgtc taagtgcaat agacaataat agccatttac gtgctatgtc tgagctagtt
    59581 gaactattgg aagaagatga gttttttaag gttttagata gtgctaaaga ggctaaagaa
    59641 attattgatt ttataaaaac acacgaatta taaaggagga tttaattatg tataaaattt
    59701 taatcgcatg tagagcagga gttggctcaa gtttaatgtt aaaaataaag actcaacaaa
    59761 ttattaagga aaataacttt ccaattgaag tagaacatgg ttcattggat tcactaaatg
    59821 gattcaatgg agatgctgta gaaacattaa ttgatgttgc tgaagaatta aaaaataaaa
    59881 atttgaagtt tgatataatt gggattcaca atattgttga ccgtaatgaa ataaagacag
    59941 cactagaaaa attccttgct tcaaaagagg gataaattta atatgataag gaggaatatt
    60001 tatggagttt attattagtt tgctgagtac acctgctgtt ttgcttggcg tagttgcatg
    60061 tataggattg attcttcaaa agaaatcagc cgttgaagta tttacagggt cttcaaaaac
    60121 acttatcggt tttttgatat ttggtattgg tgcctctgct atgacaggag cacttcaaaa
    60181 tttcaataaa ctgttccaac atggctttgg aattactggt gttatagctt caccagaagc
    60241 agctacagca cttgcacaag gtacatatgg ttttgcagtt tcatgtacat taattcttgg
    60301 attcatatta aatttagtat ttgcacgtat tacaaagttt aagaatattt tctttacaac
    60361 aggtcatagt ttattctttt cttgtgtatt agttttaatc atgaaagcac atggatttga
    60421 taatacaatc actattttaa taggtggaac tatcttaggg tttatgtctg ctgcactacc
    60481 tcaattctgt caaccattta tgcgtgaatt aacaggtggc gacgaacaag ctattggtca
    60541 ctttaacatg attggttatg gtttatcagg ttatattggg cgtttatttt caaaacataa
    60601 agatgataca acagagacta tagaatttcc taagtggtta tcaatattca gagacttctt
    60661 aatgggtcta tccatagtta tgttaatctt attctatatt gcaacattaa aagctggtca
    60721 agcatttact caagaaattg ctggttcaac acattggttg gtttttccgc ttatagaggc
    60781 atttacattt gtggcaggta tgtcaatatt gatgtcaggt gttcgtatgt tcttagctga
    60841 aataactgca gcatttgtaa tcatttcaga aaagtatatt ccaggttcac gtcctgcgct
    60901 agatgttcca acagtatttc catatgcacc aaatgcagtt attattgggt ttatatcagc
    60961 atatgctgct ggtctattag caattgctat tatggcatca tttcctagta tatttcctgt
    61021 agtaattatt ccagctgctc acatatgttt cttctcagga ggtacagctg caatatttgg
    61081 caatacttca ggaggatgga gaggggctat tgctgggtca tttgttgttg ggttattact
    61141 tgcattttta ccagttatac tatatccagt ttatgggact cttggtatag aaggagcaac
    61201 tttccctaat attgattaca acgtagttgg ttcgatatta cataatattt tacaatttat
    61261 aaaacctatt taaactaaat tatataaaag tatagtcata gttactatga tttcaaggag
    61321 gatacatctt atggaaaata cattatctga atttttagat aatatatcta atgaattgtc
    61381 agaatttatt gcctcaatag atgagacatc tataaataaa gcatgcgaat taattcttga
    61441 agctgaaaaa aatcacggac gtgtacatgt gactggtatt ggtaaaccag gacatgtatc
    61501 aggatatatt tcatcattgc tttcatctac tggaacatca gcatatatat tgcatggtac
    61561 agaagcagta catgggtcaa gtggtcaagt agttgaagga gatgttgtta ttgcaatatc
    61621 aaatagtggt gaaactcaag agttaaaagc aactcttaag actctaaaag taaatggagc
    61681 aaaaataatt ggtgtaagtg gtaatgagag ttcattttta aaaaatattt ctgatatatt
    61741 cctgttcgca ggcgttaaac aggaaggaga ttgtcttaat aaagctcctc gtgcttcaat
    61801 tttagctgaa acaatagtat tacaatcatt aagtgttgta ttacaatatg ctaaaggtct
    61861 taatacacag cagtatttga aatggcatcc agcaggaagt cttggaaagt ctattagaga
    61921 gggaatataa ttttatgaaa tataaaggga ttatatgtgc aatgataaca ccatttgatg
    61981 aaaagcaaaa tattaattca caagcaacat gtaaattaat tgattatttg attgaaaagg
    62041 ggatttatgg attatttatt ttgggtacta atggtgaatg tcatgtgtta acagatgatg
    62101 aaaaagttga atttgcaaag attgttatta atcatacaaa taatcgtgta cctgtttttg
    62161 ttggaacagg tggtaattca actcgtgaag ttatagattt atccaagaga atggaagcga
    62221 ttggagcaag tgcattatct attattacac catactttgt tacaccaaca caacaggaat
    62281 taatcttaca ttacaaaact attgctaatt cagttaattt acctattata atgtataata
    62341 tgccaggtaa gactggaatt aatattgaac cagagtcagt acgtgaatta tcaaagataa
    62401 agaatattgt tggaattaag gatagtagtg gtaaacttga caacatgaaa gcttatattg
    62461 aagtaacgca gaatgagaat tttagtgtgt tttcaggctc agattcactt attctggata
    62521 gtctaaaagc tggtagtcaa ggagctgttg cagcaacaac aaacttcctc acagagattg
    62581 gtatttctat ctatgaaaac tttatgaaag gggatttgga agaagctcaa aaagctcaaa
    62641 gtagtataga agaacttcga cgtatactaa aacttggaac aattccatct gttatgaaga
    62701 aaactattgt cttgaatgga attaatgttg gaactgcacg acttcctgta actgaaccaa
    62761 ctggggaagt acttgaagaa attaaacgag tagttgaaaa ttataggaca atacttaata
    62821 aaagtagtga gaatagataa tataaatagg gaaattacac tggtatataa ttaatatata
    62881 tattataaaa catgactaaa attaatgaat aagtaaagtg gtttgatgag tatagaggtg
    62941 ataatatgaa aaagatgaat ataaccaaaa gaatgtcaga atgtggggct cttgcaattg
    63001 taagagaaga aaatcttaac cgtgcatgtg aaattgctga aggatgtata aaaggtggta
    63061 taacagttat agaaatgagt tataccctaa ataatgctgg agaaataatt caagggctta
    63121 ataaaaagta tggtgaaata ctttgtgtgg gagcaggaac agttttggat agtgaaacag
    63181 ctcgtcatgc tatattacat ggagctcaat ttattattgc accaaattac aatgaaggtg
    63241 tagcgaagat ttgcaataga tatcagattc cttatgctcc aggatgtaca tcattgactg
    63301 aggcagtaga tgcactaagt ttaggagcga cttttataaa agctttccca atatcagatt
    63361 tctatggacc taaactagta aaggtattta agacaccaat acctgatatg ccagttttag
    63421 caagtggagg aattaatctt gaaaacctac atatatggtt agaaaatgga gttgatgtat
    63481 gtggatttgg tggtttatta actaaaggaa ctattgagga tattactgaa aatgccaaaa
    63541 agattcgtga aatcattaaa aatataagag aaatttaaca tggaggatga attagttttc
    63601 tattgacgag atatccacac attaattcct aagttagaag aaatattttc tagttgaggt
    63661 aaagtttata ttgaacttaa attaaatttt gttttaaata agatattatt ttagttgatt
    63721 gctaaaaata ctagaatcta tattaatgaa ttatactaat tttgtaccac tattaaaata
    63781 ttgtttgcta aaaataaaaa ataaccttaa aacagttgat tataaaaaat taactgtttt
    63841 aaggttattt ttatctttgt gacgaaatag acacttgcca tttagaatgt gacaatatag
    63901 tagttgttta gaaaaggtac aaagcattat actttaacta aagtaaaata tattttgctt
    63961 ttagaataaa aacaattagg agggagctat aattgaaaaa gataatatat gtattattta
    64021 caattgaact attaatagta tctttattag gtctaaatat agttaaagat aatgaaatta
    64081 ataatatttt atataacgat acaacaagta tatctgtaat gtttaaagat tataaaaagt
    64141 taaataaaaa ttatagtaag tggattaaag atattgcaga tgaaaataat gtggctatat
    64201 caaagtatgt ttttaataat aatgaaaaat tatctatata tacgactgat acaagtttga
    64261 ataacaatat aaagttaaaa tcaggaaaat ttcctaatac taattcaaat gaatttatta
    64321 gtaatataaa cactaataat agtaataata gtaatcaagt aggtaaattc tcaactatga
    64381 gtaaagacat atcaatttta attagggatt ttggtaaaat aagtgaagtt ggagaatcag
    64441 gaatcttata tatttcaact caaaatgaag atacaataaa taaaatatta agagaactaa
    64501 atgtagacag aagtattttt gtagcaagat tacatgatag atatgttaac tcaaatttat
    64561 acttaaatcc ttcaataatt agagatttaa tactaattat cctttgtttc ttggctacaa
    64621 taatacatta ttcaattagt gtgagtagag aggcttctat tttaagatta aatggatact
    64681 ctaagctaga tataattgct agggttataa aaaatatatt tagaattatg attttatcat
    64741 ctgtcacagc attattaata tatataatct atattttaaa attaaatata ggtgttagag
    64801 catgcttata ctttgctatg ttttcgatta tatgtatttt gataaatatt ttgatatcta
    64861 tattaatagt tggttttaat agtagaagtt ctaaatatgt gctatctcta aatggaaaaa
    64921 aatcttatgg ttttgtaaat ataatgcatt tcacattgaa aattgtattt gtatcatttc
    64981 ttatattatc cataaataac tgtattttaa attataatat gctacaaact cagttaggaa
    65041 atctatctga gtgggataaa gcaaaaaatg tttataatct tactcttaaa gatacaggcg
    65101 aacaatcttt aggaaaggaa gaagtaatta aaaatgccaa gattaaaggt ttctataaaa
    65161 atttggtaga agaaaaagat gctttcttaa tagattcaac taattttgaa aaacttgaag
    65221 atggaagtta catgtatgaa ataaattcaa agggtaaaaa cccagaagta agtcaatatg
    65281 gtaaaaatat aaaaataaat aaaaactatc ttaaagttaa ccctatttac tcaaatggca
    65341 aagaaatatt taatctaatc gattatgacg ataagactat aaatttatta gttcctaaaa
    65401 agttacaagt acatgaaaat gagattaaaa aagagtttag agaattattt tattttgaaa
    65461 aaatagaagt agaaaatatg tacaataaag agttaaatag agagttaaat aaaacaaaga
    65521 agtcagattt aaatgttaat ataatatatg tagataataa tcaaagctat tttacttata
    65581 actcccttgt tatggatgac aataggaatc tcattgatga cccaatagca ataattgaca
    65641 tagataatat tgatgattct ttttatcttt cttatatatc tagatgtgta tattttaatt
    65701 ctaagaaatt agatgccctt gcagatataa gtaacattat tgaagctcaa ggtgtagaag
    65761 cgcatatcca aagtttacat gctgtttata atgagtatgg tttagaaata aataaactag
    65821 aaaaatattt gaatagtgaa atttttacta taataataat agcaatatca aacttaatga
    65881 ttacctacaa tatagtagct agctactatg aaagaaataa atataagtta tatattaaaa
    65941 aaatatttgg ttattcaact acagctagaa gtatgttgtt aatagtctca ttaatattaa
    66001 caaacattat tccaataggc attatatcaa caagagtaga tttaagtaat aatattatct
    66061 tatttggact tctgatatta gttatagaag taattgtatc tattgtatta gacaagatta
    66121 tctcaaatag ttcatttaat aaaattataa aaggagaaca ttaaaaagat gatttcatta
    66181 ataaatatat ctaaaaaatt taaagataaa tctataatag acaaatttaa cctagaaatt
    66241 tctaagggcg agtttgtagc gataacagga aatagtggtt caggtaagac aactttactt
    66301 aatataatgg gattattaga aaaacctaat tctggtgata ttataataaa taatataaaa
    66361 aatccaaata gtaaacagat aagaatttta caaagggatt tttatggcta tctatttcaa
    66421 aattatgctt taattgagaa tgaaaatgtt gaaaataact tgaaaattgc tttaaagtat
    66481 caaaaaaata tcaaccataa tgaaaaaata aattacgcac ttgagtctgt aggtcttaga
    66541 aaatacaata aaaaaaagat atacgaattg agtggaggag aacaacaaag ggtagcatta
    66601 gcaagagtaa tattaaaaga atgtgaagta atatttgcag atgaacctac aggtaattta
    66661 gataaaaaaa atagagacat agtatttgat atacttaaaa atctaaataa gaatggtaaa
    66721 actatcgtat ttgtaactca tgatttagag ttatcggagc aggcagatag agttataaaa
    66781 ttgtaataat atcaactata ataaatataa gaaagagatt acagctaaat caagtttaat
    66841 aataatacga atagaattta aacattaagt aaattagtag tttgtaaata taagtataat
    66901 atgatatcat aaaggctcaa aagaaatttt atataataag aaatttaaaa ataacaaatg
    66961 gaggacatat aaagatggaa aatttaccaa attgcccgaa atgtaattca gaatatactt
    67021 atgaagatgg aactctttta gtttgtccag aatgctctca tgagtgggca ttaggattgg
    67081 gagctgaaga agattcaaat attatcaaag atgtaaatgg aaatgtatta aatgatggag
    67141 attctgttac agtaattaga gaccttaaag taaaaggaag ttcatcatct ataaagattg
    67201 gaacaaaagt gaaaaatatc cgtttaatcc atgactcatc agatggacat gatatagaat
    67261 gtaagataga tggttttgga gctatgaagc taaagtcttc tgttgttaaa aaagcgtaat
    67321 attataaata agctgtagtg tttattgttt ttcagtacag tataaaacca taagaatcta
    67381 ttaagtaata aaagtttgtt tatttatctt tatattttct tatggtttta ttaaatataa
    67441 attttattga acaccaaaat attctctcct ctacaaataa gccaatctta tttaaat
//
LOCUS       DAELXX010000006        62796 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.006, whole genome shotgun sequence.
ACCESSION   DAELXX010000006 DAELXX010000000
VERSION     DAELXX010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 62796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62796
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.006"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..483)
                     /locus_tag="KPH29_000369"
     CDS             complement(<1..483)
                     /locus_tag="KPH29_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HBG1466010.1"
                     /translation="MIFKKYIKSLLIVVSVTLIMNKSILFVNALDLESNNNIKSYKRK
                     NVKEIDISNKFVDESFRRDIVEKVLDNRDDPLTNEVASKDNYKIYESDLNKIESCLEL
                     DLHKNPDVSNPNKIDNLDGIENFRNLEKLDCSSNNLESVDLSKNIKLKYLDISGNKIL
                     N"
     gene            complement(577..1389)
                     /locus_tag="KPH29_000370"
     CDS             complement(577..1389)
                     /locus_tag="KPH29_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG1466011.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADTFRKE
                     GHKADVIICPEKQFKTKAEEKTYKIPRVNSGGYDLLIELHLNASDGQGKGSEVLYYSN
                     KGLEYATRICNKLGTVFRNRRAKLDKGLYILNSSNPTAVLIESFFCDNKEDYEKAKKL
                     GHEGIAKLIVEGVLNKNINNEGVKQMYKHTIVYDGEVDKISATVVGWGYNDGKILICD
                     IKDYVPGQTQNLYVIGGAACEKIGSMTKEKFTMIKGNDRFDTLYKALDFINR"
     gene            complement(1468..2331)
                     /locus_tag="KPH29_000371"
     CDS             complement(1468..2331)
                     /locus_tag="KPH29_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HBG1466012.1"
                     /translation="MVEVKEEKTFDEQIDILKSRGLIINDKEDAKFVLSNVNYYRFTA
                     YLLSFKNDDGSYKEGTTFEEVYDIYRFNKEFRILLTDLLGSIEIAFRTYIAYTLAIKH
                     GACGYLERESFKDEKFYINFLTALEREKSNNSDKLFIIHHKEKYEGKLPIWVATEIMT
                     FGMLSKLYSNMLPEDTRYIKNNLCRVNTLLVKSWLQSLTQVRNQCAHYGRIYNNNFRI
                     ITIKNEYKKYNLDNKKIFSYILAMKHLTMDKLIWNSFFIKLQKLINDYNNSIDLKLIG
                     FPNNWIEILAK"
     gene            complement(2592..2849)
                     /locus_tag="KPH29_000372"
     CDS             complement(2592..2849)
                     /locus_tag="KPH29_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBG1466013.1"
                     /translation="MDNLISFIPEQLLILVAALSIIGKGCKKYKQLDNKYIPVVLLIL
                     GIGFSIWMLGLNPVAVLQGVICWGVAIGINQTYKQLKDGEK"
     gene            complement(2853..3143)
                     /locus_tag="KPH29_000373"
     CDS             complement(2853..3143)
                     /locus_tag="KPH29_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466014.1"
                     /translation="MEEISINLLCAVAGVVISYLAFRNNSNRKIQDETETTTKLEQQI
                     TFLCENVRDIKHDVAKFNTSFLDISERVAKIEASTKQAHLRIDEIVNRIGGK"
     gene            complement(3174..3740)
                     /locus_tag="KPH29_000374"
     CDS             complement(3174..3740)
                     /locus_tag="KPH29_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466015.1"
                     /translation="MTFKELVNKVRNLVLEAKNVTIEDTESKFTSENVEGALKECIDR
                     ADEAFQEADSGKTLLSTAIGSPTTSEQTFQDYANYITGFKSNISNLETQLKSKYSIRY
                     GPIDGYDGNPFSANFGKSASYLIVYVYFRRSVYYYNPSGSSLGSNTGGSERAWITINS
                     NKTGFSVHSYDTSYESYPFTGYYIACFA"
     gene            complement(3774..3956)
                     /locus_tag="KPH29_000375"
     CDS             complement(3774..3956)
                     /locus_tag="KPH29_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466016.1"
                     /translation="MNINNVVVRILAERILSKGLNPLKNREFQLDDVTNTEYRKAVED
                     YIIKNSGVVEGAEPTI"
     gene            complement(3956..4249)
                     /locus_tag="KPH29_000376"
     CDS             complement(3956..4249)
                     /locus_tag="KPH29_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466017.1"
                     /translation="MNVPNRIIYDQTGRTIFETGESCGDVLPHYTITELHYIDIEYGS
                     IDYTRNRVIGINIETKEPILEEIPVYITDEEKRIQELENQLLIAENEKVGGLL"
     gene            complement(4264..5790)
                     /locus_tag="KPH29_000377"
     CDS             complement(4264..5790)
                     /locus_tag="KPH29_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466018.1"
                     /translation="MALSMSYFNLPDKRKYTKNLAFNPFAGGRQNFEWTGGDHGLNGE
                     FKETCLSCTYNGSTLNWGSGNVWVLGEYGQYTFTYNCESMHVDTQQKFPYTSNRIITI
                     KGRPVISGSDTSLGNKRKGFSVDFTVSDDTPNVNLIVRAYLDDKLIQNITPVVQNSTL
                     TATVTDSQLNSLSVDGNHKLKIQLNDGYDNFDRIFTFKKIEKGIDISTSLVTDSQAKF
                     TVTKIYSELTKIECYLDETLKETFTTDLYSEKTINYELIDNAIHTLKIVVTDAENVVE
                     EKVISISKNIMPLQPDATLQDISTKLTEIGQGVRNGKTSIINTLALKNIDASLNNTLV
                     ELSEKIKGGFDSGDASLQDLMNQLTQANNTISQLNTKYKVASGTVTSFADSTKIAYPY
                     LTDNVTKPGSWIKVSNLGFKPNIFFADFDYYDAEYKNNYKLFLFACNGVATQRGVDFS
                     SVTSFIRKSGDEYFHANGWLYSNSEGDVYFNNTGVQIPAYNFDSTQKHTYKWYAIKFI
                     "
     gene            complement(5807..6832)
                     /locus_tag="KPH29_000378"
     CDS             complement(5807..6832)
                     /locus_tag="KPH29_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG1466019.1"
                     /translation="MAIDKSYYTIITDVGKAKIANASVTGNKVGFVKIQLGDGGGSEY
                     TPTESQTELKNVVWEGNIGNTTTDETAPNCIILESLIPSSVGGFMIREIGYLDDENNL
                     IAISKYKECYKPSIEQGAVVDMKVKTVLIVSNINNIELKIDPTIIFATLKDIQDLDAK
                     ISNVNTKIDTTKTELTSNIETAKTELNTRIDTENEKQNIKIDQLIAGGSNVASTQTIT
                     IDDWIDNQEGGFKATVTHGLLTQRITVSIIDATTKDNVVPDFTIIDDNSIEVRSGVKV
                     ELNVYVINGNAETHFINATVDDNRVSEMTTYSSKKIHEEIGKVAEQLTGINSNIISTV
                     NNDILPI"
     gene            complement(6844..7461)
                     /locus_tag="KPH29_000379"
     CDS             complement(6844..7461)
                     /locus_tag="KPH29_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HBG1466020.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVENKEIKTLHDKLKDLSN
                     DQFLEDLTISGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDNV
                     VGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSNIKPCNMTLDYTRVLISKDNY
                     MNFGITTLMGQEITIYPWSPPDIETYGEIDVLTGNGVGYQEITIF"
     gene            complement(7454..8503)
                     /locus_tag="KPH29_000380"
     CDS             complement(7454..8503)
                     /locus_tag="KPH29_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBG1466021.1"
                     /translation="MFELMTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAIELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLEATHTIAKGVFNIDIPLDSRFNLGE
                     YNYVAIERISEKTYKMKCETTGPVFELGKLIPIEYIDGLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNIQNYRDEVNKIQDVGGVKVYPTWDGGGTVKLVIINSNFKV
                     PSEELVNLVQEEIDPIGHQGEGLGLAPIGHRVTVEGVTSTTINISAEITYKNGYTWEN
                     IKSIAEEAIDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDIANTMINDVKSNL
                     TIDSNSIVVRGEVVG"
     gene            complement(8504..8923)
                     /locus_tag="KPH29_000381"
     CDS             complement(8504..8923)
                     /locus_tag="KPH29_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBG1466022.1"
                     /translation="MLPSDNLDYDIEDVSIINFDVRQEPSKTFKLHIEKSKIDGICDD
                     VEALKQTIFLILNTERYEHLIYSRNYGVELNDLIGEPISYVIPELERRITEALIQDDR
                     IENIDNFEFQNIKGKVQCRFSVHTKYGNIKAEKVVSV"
     gene            complement(8928..9188)
                     /locus_tag="KPH29_000382"
     CDS             complement(8928..9188)
                     /locus_tag="KPH29_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HBG1466023.1"
                     /translation="MSQDLLQIIKKAAMDAVETSNPMRVVFGTIESISPLRVKIEQKL
                     SIGEIFLIQTDTFKRYTDKKMGDKLVLIRMQGGQQYLILDRM"
     gene            complement(9202..11094)
                     /locus_tag="KPH29_000383"
     CDS             complement(9202..11094)
                     /locus_tag="KPH29_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG1466024.1"
                     /translation="MVDELVLANDRDVRLVIAHWEDFYEPAVIDGITWEIERRGTPSK
                     LEFTIVMDDILEFCEGNSVRLYYKGIGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKILAKDFNLKYNVIEDTKYKISRVEENKTLFDMILTALDDTLRE
                     KKEMYTLYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     SGKRDVYIAQDSAHMRSWGILQMFDTVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     ALGDIRVRAGCLVPVFLNLGDIELQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKDEKKNGPAQSIMKKNTGKKVPAIFTAYYPGNNAMEGGKTDCNGKPL
                     DVKSRTVAGPMNREGVKKTWYTDDFLNKHPVFEYGDKVKIILPGTAYDNKVYTVKDNG
                     GRIYVETNGTYHIDILLANASECKKFGRKNGYIIIGGDEEQTYQVEGNNQSNTNNNSK
                     EDKLISIAKSKLGCNYVYGAEGPNNFDCSGFTQWCYKQIGIKIPRTASAQSKAGKAVD
                     LKDRSKWKAGDLLCRIGGGSSNHVVMYIGNNQIIHSPQTGDVVKIESVNSYRKGKAYT
                     HVRRFI"
     gene            complement(11087..11776)
                     /locus_tag="KPH29_000384"
     CDS             complement(11087..11776)
                     /locus_tag="KPH29_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBG1466025.1"
                     /translation="MAYDFYLDGVQLPIAPPKLEVKVTNKNKTVDLINTGEVNILKKE
                     GLSEISFEAEFTHNKLPFCRGQFRDVQFFLSKLELLKTDCKPFQFIVSRELGNKVLFN
                     TNIKVSLEEYAISEDADNGSDTKVAIKLKQYRDYSTKKLVLAPPKNETGRPNVKIEPK
                     RVDSVNATNTKTYTVKAGDSLWSICQKQLGNGSLYKKVYELNKSMMDKANKGKNLSKY
                     TIYKGQVLKLG"
     gene            complement(11793..14165)
                     /locus_tag="KPH29_000385"
     CDS             complement(11793..14165)
                     /locus_tag="KPH29_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBG1466026.1"
                     /translation="MATIQTSIKIFDGMTPAFRNMTTSINTTINSLERLQQRLHNPLN
                     TGGIQASQQSLNNIENILTRIEQKIGRNTNEQENFNNKIKQGSEAGSLLVSKLKSLAG
                     IYIGIRGIESITKAADTIASTKARLNLMNDGLQTTEQLNKMIYLSAQSARASYADTAA
                     QVAKLGILAGDAFGSSAEVIKFTELMNKAFVIGGTSANEASAAMYQLTQAMGAGKLQG
                     DEFRSIMENAPLLATKIADAMGKTKDQLKELSSSGAITADVIRNALFKASDEIEKKFA
                     SMPVTFSQALTMMKNDAYMIFGQTLGKISGALQSVRFSEIVVSIRNVMIAISSNIYDT
                     LNIIKNILNSEFFSNLVQGFTFCAVVITKSLGSIVNTALNIVNIFAQNWSIIKPIVFG
                     VASVFILFRGVLLATKIATIGNVIANVAHAASSSLSALMTNIQAAALMRSNGATLSAT
                     IATWGLNAALLACPITWVVLGILAFVVVVFVAVAAVNKFAGTSLTALGVIVGAVFAAV
                     AAIQNVMIWLLNGCIAVNEGITNGWNQCVYLMKQAIAKGVIFIIEKMASLNNSVNNAG
                     NALGKAFIDGANIAIRGVNKLIDLINKIPGINIGKVGEATFTPVKADNSYIKQQIDSL
                     NRWVGDAPEKVKLERMGYKDIGAAFQKGNALGTKWQNAITDKFKDTFDINKMLEDAKK
                     KLGLDDLWNKQNPLNNLGGFGGDLGKNVKDTAGNTAKMAKTMDKSQEDLKYLRDIAEQ
                     ETINRFTGVNIKIDMNNTNNISKDTDVDGIVNVLTEKLNDAMVVSAEGIV"
     gene            complement(14231..14998)
                     /locus_tag="KPH29_000386"
     CDS             complement(14231..14998)
                     /locus_tag="KPH29_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4428 domain-containing protein"
                     /protein_id="HBG1466027.1"
                     /translation="MGLFGKKKADICCICNTEIGVLNIEDGWICNSCFKEYCDALSMT
                     KAPKILRKLDIEKTISSTKKNNELQKIFNTTNNIENYIEFDEDNKKWLVSKKSINDKK
                     TPIIHSYEDIVKFELLENGETVTKGGIGRALAGGILFGEAGAIVGGITGKKTTRKVVD
                     TFKIKITINNIDNPIEYIELINKKTKTNSSAYEKAYKDAHKILSTLSAITQSIKETDN
                     INTKSVADEILKYKNLLDMEAITQDEFNTKKKELLNL"
     gene            complement(15136..15663)
                     /locus_tag="KPH29_000387"
     CDS             complement(15136..15663)
                     /locus_tag="KPH29_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HBG1466028.1"
                     /translation="MREGKRRRGCLFWFILIILFSGVVGAIAGNSTNNESTEKQKEDL
                     TKYIGEEGSIGDLKLTVNSISKASEISVASGYLAYTPDSGKYGIINLTIKNQTKESQS
                     FMLNSFTLIGPDDSKYVPSLLIDVGNKYITMDTVNPNLDVTGNIAFEIPKDLLVSDCK
                     LKYSGSEQEFILKEK"
     repeat_region   15786..16075
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=15916..15944
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(16528..17394)
                     /locus_tag="KPH29_000388"
     CDS             complement(16528..17394)
                     /locus_tag="KPH29_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
                     /protein_id="HBG1466029.1"
                     /translation="MKNLIVKEFNGSQIYTFMWKEKSCWIANQIVGLFDYADVSKTIQ
                     DCIKAEDFEIEQEYDVLKGNEFNDFVTTLNVVANNIISNKARSITIFYEDGLYGFLQY
                     TDKPIGVQFRKWLRREVLPSIRQTGAYITNNANPEKLREKASEIEKLQLAYNSTSMLK
                     ELLDGAGFDNKSKLLTAKTLYKKAGIDLPIEINEEEHYFDTKQIASKLKIYSKSNKPA
                     QLAVCEIIKKIDLEENEVKGVWETNGSWTGTVNKYTKSVIDKVRNWIEENNRPTKIAG
                     EKKNYHVVYKIE"
     gene            complement(17513..17701)
                     /locus_tag="KPH29_000389"
     CDS             complement(17513..17701)
                     /locus_tag="KPH29_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466030.1"
                     /translation="MKIITDSSRADYFKQRRQNKKTFSVLLDREKVEKIEEHLKKQNK
                     TKTIWLEEKINEELEKEE"
     gene            complement(18841..19002)
                     /locus_tag="KPH29_000390"
     CDS             complement(18841..19002)
                     /locus_tag="KPH29_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466031.1"
                     /translation="MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFD
                     FKIKFHKFK"
     gene            complement(19343..19783)
                     /locus_tag="KPH29_000391"
     CDS             complement(19343..19783)
                     /locus_tag="KPH29_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG1466032.1"
                     /translation="MSNLSAFLSQNAIKVDNVKYVASNRFLDKEGKPVEWELKVLSSE
                     EDEALRRKCTKRVKVIGNNGKHTGQYTSEIDYNSYVAELCVASTVFPDLKDAELQNSY
                     GVMGEAQLLKTMLTAGEYVNYTVKVNEVNGFDTSFEDKVEEAKN"
     gene            complement(19855..20682)
                     /locus_tag="KPH29_000392"
     CDS             complement(19855..20682)
                     /locus_tag="KPH29_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466033.1"
                     /translation="MEFESGIKLGKLTLIKYLGRNKHSKKVWLCQCECGNKVERVENN
                     LKQSIKNEKPPHCGCSPNWKGQNKRFKDITGKTFGKLTVLKMSGKDKYSHNLWLCQCE
                     CGNRTITSTSALEQGKAQSCGCIRKEILLERSTTHNKSNDSLYRVYHRMIKRCTNKSN
                     KDYNYYGGRGIEVCNEWLEDFINFYNWSIENGYRKGLTIDRINVNGNYEPSNCRWVTW
                     EVQRNNKRNSIRVNYKGEMITLKQCAENLNVKYLFLYNQLVTKKIPLEEVIKNIGME"
     gene            complement(20741..21211)
                     /locus_tag="KPH29_000393"
     CDS             complement(20741..21211)
                     /locus_tag="KPH29_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBG1466034.1"
                     /translation="MAQTINAKDTVSAKKAECFITIEGKRYNFMQAIDLEAKMEKNKS
                     EVPILGRTTKGNKTTGSTNTGSATFHYNTSIFRELLYRYKETGEDIYFDIQVTNEDPT
                     SAVGRQTVVLKDCNMDSGIITKFDADGEYLDEDMDFTFEDWELVESFALLNGML"
     gene            complement(21228..22538)
                     /locus_tag="KPH29_000394"
     CDS             complement(21228..22538)
                     /locus_tag="KPH29_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HBG1466035.1"
                     /translation="MALGGGTFVTQNKILPGAYINFISAKRATSSLSDRGIVAMPLEL
                     DWGIDEEAFQVTSDDFEKYSVKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CTIATAKYSGIRGNDLKVTVTTNIDDNAKFDVVILLDNKKVDTQIAKVITDLQDNDYI
                     TWKKDATLEASAGLVFTGGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTTEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKSDADYEGVVSIENKIKDKDLVESSLIYWATGAIAGC
                     DINKSNTNKKYDGEFDVDVNYTQIQLEEALKTGKFIFHKVGDEVHVLEDINTFVSFTD
                     DKNDDFSSNQSVRVLDQIANDIATLFNEKYLGKVPNDKAGRISFWNDVVKHHKELENI
                     RAIEDFKTDDVSVELGNDKKTVIVSDAIKVINAMSKLYMTVSVN"
     gene            complement(22539..22715)
                     /locus_tag="KPH29_000395"
     CDS             complement(22539..22715)
                     /locus_tag="KPH29_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466036.1"
                     /translation="MSKALSKEDNYKFTKEQIVNSKKYVNRKDLLNAILKENELYSFS
                     EVEDRINKFMKGVS"
     gene            complement(22708..23145)
                     /locus_tag="KPH29_000396"
     CDS             complement(22708..23145)
                     /locus_tag="KPH29_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466037.1"
                     /translation="MLNNIIDGISVKLDKSFGEKYTIYSEDVEQGINEPCFFIVPLNP
                     SKTPYPSGRELKKNSFDVHYFPRSEDKNFEINEIAEMLLEELEYIEIDGDLVRGTNMN
                     FEIIDNVLHFFVDYNYFTIKNNDTNKMDTVELFGGLKRGDNFE"
     gene            complement(23494..24300)
                     /locus_tag="KPH29_000397"
     CDS             complement(23494..24300)
                     /locus_tag="KPH29_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466038.1"
                     /translation="MAKFLDITGKKFGRLRVIKFSKEIKSGKRNRKYWLCKCDCGNFK
                     EIRTDSLTSGLVQSCGCLKKEQDKLNLTDKYQFKKKYKVQNKRLYSIWKGIISRCTDK
                     NNKRYNRYGERNIIVCDEWFCYDNFANWALSNGYSEKLTIDRINNEGNYESSNCRWVD
                     IKTQCRNRSTNILVKHEEKEITLIELSEKTGISYSCLRSRYSKGLVGNKLIEKVKIIE
                     ESRAKLSIEDVKEIRKKYSDGYTIKQLSEIYPVTYSSISNIVHRRTWKNI"
     gene            complement(24410..24700)
                     /locus_tag="KPH29_000398"
     CDS             complement(24410..24700)
                     /locus_tag="KPH29_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBG1466039.1"
                     /translation="MARWGSVDFREFKRVCKKMEKFTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLREGWSGVAYARSLPVYKQGNNYIIEVVNPTEYALTQWGI"
     gene            complement(24700..25047)
                     /locus_tag="KPH29_000399"
     CDS             complement(24700..25047)
                     /locus_tag="KPH29_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466040.1"
                     /translation="MVSKTRKAIEMLYRDKCTIVEYQPIKDPVTKRTNNKEVIVLENQ
                     PCKLSYKNIVSATEGKLAKLEQTIKLFISPDIEIKAGSKLIINDKEYVRSGESAIYPN
                     HQEIILELFKDKA"
     gene            complement(25041..25442)
                     /locus_tag="KPH29_000400"
     CDS             complement(25041..25442)
                     /locus_tag="KPH29_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466041.1"
                     /translation="MENNIIDEIEKRLESFGYILKDGDKWLIGFVREKIENIIKLDCN
                     IKTMPIELKEIEVDMIVGEFLFTKKNMGQLDIESINFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDSLIAYLTTYGKNKILTFRCLRW"
     gene            complement(25452..25685)
                     /locus_tag="KPH29_000401"
     CDS             complement(25452..25685)
                     /locus_tag="KPH29_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466042.1"
                     /translation="MPQVKKLNRILTIEECKIDDFLEMGYDLIDETGKAVKYGKSLSV
                     KDLIAENNILRSKVESLEEENKQLKEKNKLTKK"
     gene            complement(25704..26594)
                     /locus_tag="KPH29_000402"
     CDS             complement(25704..26594)
                     /locus_tag="KPH29_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HBG1466043.1"
                     /translation="MAALNYAKEYSNVLAQAYPYTLNFGDLYATENNGRYRWTGSKTI
                     EIPTISTTGRVDSNRDTIAVAQRNYDNAWEPKVLTNQRKWSTLVHPADINQTNYVASI
                     GNITKVYNEEQKFPEMDAYCISKIYADWTALGNTADTTVLTTANVLEVFDKLMEKMTE
                     ARVPENGRILYVTPVVNTLIKNAKEIQRTVNIKDGGTSLNRQTTDIDTVKIIKVPSNL
                     MKTAYDFTTGWKVGAGAKQIFMSLVHPSAIITPVSYQFSKLDEPTAVTEGKYFYFEES
                     FEDVFILNKKADAIQFVVEA"
     gene            complement(26606..27169)
                     /locus_tag="KPH29_000403"
     CDS             complement(26606..27169)
                     /locus_tag="KPH29_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HBG1466044.1"
                     /translation="MDWLKELLEGIKVEDNKIDVASLQKSIEKKIKETTVTQEDYVNL
                     ETQLNTANETIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFNYWLGDAF
                     KSVKCRDEIALKAHLDMEALRNSKDRQKAFEEQINPLKQDKDYLFNATLEGEEPKIDT
                     ITPGQEPKINDFGFNFTGVRPHENNNK"
     gene            complement(27237..27485)
                     /locus_tag="KPH29_000404"
     CDS             complement(27237..27485)
                     /locus_tag="KPH29_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466045.1"
                     /translation="MDNKFKNLQHLIDSIDLGLDIEFDLYNKPYNISIGDDDTRFITL
                     CPNGDTKYYKNGKDMVDNYNIDGKLLKDLWKDIQIVNM"
     gene            complement(27487..29022)
                     /locus_tag="KPH29_000405"
     CDS             complement(27487..29022)
                     /locus_tag="KPH29_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBG1466046.1"
                     /translation="MKHKDYWRKRFEQLEEVQNNKSVKYYLELEKKYKLAMNSIEKDI
                     LSWYNRFAENEGISLLEAKKLLNTRELEEFKWNVEEYIKYGKENAINQKWMKELENAS
                     ARLHITRLEALKLQIQQHVEVLYGNELDGIDKLMRDIYTSGYYHTAFNVQQGVNVGWS
                     LMSLDTNRINKIISKPWATDGLNFSERIWGKHRPTLINELHTKLTQSIIRGENPKNLV
                     NDFAKRFNVSKSQAKNLIMTESAFFASASRKDCFSDLDVEKYEIIATLDLRTSNICRE
                     LDGKIFDMKDYQVGITAPPFHCRCRTTTAPWFEDEEGYRAARGEDGKTYYVPSSMKYN
                     EWYEKHIEGRLGKEKADTLRKMQLNESKDRKQFEEYKKVLGNEIPSKFDEYQHMKYND
                     TIRYEEAKKLYKDVNWQVKNQRNLTSGSVHSVPFESKPNSVFDNYKDGKLIQRRYYGN
                     TGKPRLDLDLTDHRNPKQHKIVPHKHDWLADENNHDKVKREKDMELTKAHKIANKDIL
                     NGE"
     gene            complement(29012..30454)
                     /locus_tag="KPH29_000406"
     CDS             complement(29012..30454)
                     /locus_tag="KPH29_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG1466047.1"
                     /translation="MLNIYISETDLIKVQLKKESTFNLVKVIEHYILKHRPEKYKQGE
                     EYYYGNTDVNNKRRYYLLDGAKVDDFTKVNNKAINNYHKLLVDQKVGYSVGNPIVFNA
                     DDDNLTKLLNDLLGEEFDDTITELYLNASNKGVEWLHPYINRKGEFKYVIIPAEEAIP
                     IWDSKRQRELVAFIRFYYIEDIDGNKIKRVEYYTENDVTYFIERGNSFIQEFLYDEYG
                     KMTDIQEGHFRINNKEQGWGKVPFIPFKNNEKCVSDLTFYKSLIDIYDNNISTLADNL
                     DEMQEVIYVLKEYPGTSLQEFIDNIRYYKSIKVDGGGGVDKLEINIPVEAKKELLDRL
                     EKNIIIFGQGVNPESQNTGDKSGVALKFLYSLLDLKCSKTEKKFKKAIRELLWFVCEY
                     LKISGSKSYDYKSVQITFNHSMIINESEKIDMASKSIGIISDETIVSNHPWVEDVNDE
                     LERLKKQEETQKEYDDLIPTNNQEGVIDET"
     gene            complement(30460..31713)
                     /locus_tag="KPH29_000407"
     CDS             complement(30460..31713)
                     /locus_tag="KPH29_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBG1466048.1"
                     /translation="MVIEMIVRINFNPDFKEANFTKKRYRAMKGSAGSGKSVNVAQDY
                     ILKLGDKKYQGANLLVVRKSEATHKYSTYAELTGAINRIYGKQADKYWKTTLNPLEIK
                     SKVTGNSIIFRGVNDAKQREKLKSINFSKGKLTWVWCEEATELMESDIDILDDRLRGI
                     LTNPNLYYQMTFTFNPVSATHWIKRKYFDYKNDDIFTHHSTYLQNRFIDEAYYRRMQM
                     RKEQDPEGYKVYGLGEWGETGGAILKNYVIHEFPTEFEYFDNMRLSQDFGFNHANAIL
                     RIGFKDGELYICNEIYVHEMDTSEIIKIANSRGLEKTLFMYCDSAEPDRIKMWKSAGY
                     KAKGVKKGPGSVKAQIDYLKQLRIHVHPSCTNTIKEIQQWKWKQDERTGLYLDEPVEF
                     MDDAMAALRYSIDNKLKNNGISFLK"
     gene            complement(31691..32383)
                     /locus_tag="KPH29_000408"
     CDS             complement(31691..32383)
                     /locus_tag="KPH29_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1466049.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEISAKYDISLNTLKSWIKRYNWSSE
                     KKKGAPINKRGAPFSNKNSVGYGAPKENKNAEKFGFFSKYLPEETRELIQEISIKDKF
                     DILWEQITIQYAAIIRAQKIMYVKDKEEMIKELKKHEITENSEKIEYEFQFAWDRQAS
                     FLNAQSRAMSELRSLIKQYDEMIHKDWNLATEEQKTRVEKLKCEVANLNKDDIGDDEL
                     KISVDYGDRNDS"
     gene            complement(32453..32962)
                     /locus_tag="KPH29_000409"
     CDS             complement(32453..32962)
                     /locus_tag="KPH29_000409"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466050.1"
                     /translation="MYKKVLNILLNYYKAINSKEVVELVLTPVLICIISYMFFNSSIN
                     KEVVISLNKDVLTLSGLLVAFGVCIITLLFTTYNKSISEAKDIKTERKVNGFNISYFQ
                     FIQLKAYYTVIMEILVVVICFINTIMLTRYYSNIIFYVLIFFTVHIILSLTTLIISMF
                     HLSWKDRGD"
     gene            complement(32983..33957)
                     /locus_tag="KPH29_000410"
     CDS             complement(32983..33957)
                     /locus_tag="KPH29_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466051.1"
                     /translation="MSNFKKRYIDVYRIDIINKINGSVYSDEEILKEVIKTFFKDKEY
                     CKENKNGINITKDKKFWITEVKCSEDDLIIKVKLEYTKYNQNTNIINAHTKEVEGNKK
                     MDQGDSNKQHLFIKFMKENNIAVVLFERVFVGVPMIELSKNLHLYYEDMIENNKFDNN
                     MSKTSINITQIASKDFIEQILDLDGVSKITLNVDREKFGTDEDNLFSNLNNSRRHNDL
                     IYKPTFRTRYLPSEVKRCCEKYIKGEEFRNKKINRIIIEGEKNKRKLKLDTEGIKLCR
                     EIEVSLDIDNHIDSEDIFEKMNNLIDDLIENNSVFFNLMYQEVAMSEA"
     gene            complement(34152..34701)
                     /locus_tag="KPH29_000411"
                     /pseudo
     CDS             complement(34152..34701)
                     /locus_tag="KPH29_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994390.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
     gene            complement(34984..35463)
                     /locus_tag="KPH29_000412"
     CDS             complement(34984..35463)
                     /locus_tag="KPH29_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 region 4 domain-containing protein"
                     /protein_id="HBG1466052.1"
                     /translation="MDKESIKEIVRELRKEEREDRKKGVLYNTRLLMKHYNDFKRHID
                     SAVSEAKDVDYLEDDLGKLDNEELYILSIKKSKARTIIMIAHIDSAMKTLKLRQERLH
                     SYEKYRALELYYCDEKTYEEIAEILNCGVVTSRRWINEMVKELGVYLFGIDGLKLTV"
     gene            complement(35456..35593)
                     /locus_tag="KPH29_000413"
     CDS             complement(35456..35593)
                     /locus_tag="KPH29_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466053.1"
                     /translation="MELISPGKIVRCAWCGRRFYKLDKSKVEYCSRSCSKRARRSDLS
                     G"
     gene            complement(35617..36987)
                     /locus_tag="KPH29_000414"
     CDS             complement(35617..36987)
                     /locus_tag="KPH29_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG1466054.1"
                     /translation="MEFKPHPYQEYTIRKTIDNNNIGLLLDMGLGKTVCTLTAISELM
                     YDYFDISKVLVIAPLRVARDTWSSEVKKWEHLKHLKVSKVLGSKLDRVRALSTDSDIY
                     IINREMVPWIVDFYKRKWPFDMVVIDELSSFKSNKAQRFKSLKKVLPLTKRVVGLTGT
                     PTPNSLIDLWAQMYLLDRGERLGKTITGYKERYFEPGQKNYQTGAIYNWQPKNGAENA
                     IHNKIKDICISLKAEDYLNMPRKIDNKIEIHLDSKILKYYKELEKEKILELDRDIITA
                     SSAAVAANKLLQLANGAIYDNDKNVKELHREKLEALKEIIDVSNGKPIIVFYNYKHDY
                     NRLMKEFKSLKPRTIENSKDIYDWNNGRIQLLLCHPASTGHGLNLQSGGSIIVWFGLT
                     WSLELYQQANARLYRQGQRETVIIHHLICKGTIDEQVMEAIENKDKGQSALLEAVKAK
                     LKEYRE"
     gene            complement(36936..37262)
                     /locus_tag="KPH29_000415"
     CDS             complement(36936..37262)
                     /locus_tag="KPH29_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HBG1466055.1"
                     /translation="MLESKIEKRLKKEIELLGGKAMKFISPGEAGVPDRIVLLPEGHV
                     IFVELKAPGKKLRKLQQYKMRELRELGFKVKCVSTLKEIDDFIKEVKGWNLNHIHTKN
                     TQLEKL"
     gene            complement(37543..39960)
                     /locus_tag="KPH29_000416"
     CDS             complement(37543..39960)
                     /locus_tag="KPH29_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466056.1"
                     /translation="MDIKASEIEHINVRHDGQLMLAIGKNKLETHWKNKSILWSELVN
                     RLSKTLRTQETYTEYRKMSKTEKDRVKDVGGFVGGSLKNGRRKAENIANRSIITLDID
                     YANKDIWEDITLLNDYACLMYSTHSHTENNPRYRLVIPLTRPVLPEEYQAISRMIADT
                     IGIDMFDDTTYQPHRLMYFPSTSIDGDYIFKFQDGEFLNPNEILDLYLDWTDVSYWPE
                     SSRERQKFNTQLKKQQDPIEKAGIIGAFCRSYSIKETIENFLNEVYIPGIDETRYTYS
                     EGSTSGGVVIYDDKFSYSHHGTDPASGILCNAFDLVRIHKFGELDEDAKPETPVNRLP
                     SFTRMSEFASSDTKVRKTIGRENLDKAKDDFGDIDFEDDEWLTRLDYDNKGSYKKTTN
                     NILMFIENDPYLKGKIAYNEFSNRAVVLGKLPWRKDDKLNDWNDSDDSGLRHHIETIY
                     NISSPSKVNDALIIAFENNTFHPIKDYLNSLKWDGIKRVDTLLIDYLGAEDNHYTRTI
                     IRKVLVAAVARVFNPGIKFDNMMVLSGPQGMGKSTFIKKLGGDWYSDSLTTVQGKEAY
                     EQLQGVWLLEMGEMMATKKADIEAVKHFLSKSEDIYRVAYGKRTSRFLRQCVVIGTTN
                     DKEFLRDKTGNRRFWPIDTGVKKVNKSIFNGQLDNERNQIWAEAVELCKANEQLYLSD
                     EEKKEAERQQRTHSEENAKSGIIEEYLNKPITENWYDLSISEKREYIHGSDFGDLKEG
                     TILREKTCVMEIWVELFNGEPKQLTPILSREINDILKGLDDWISYNGNLRFGKIYGRQ
                     RAYVRKS"
     gene            complement(39972..41930)
                     /locus_tag="KPH29_000417"
     CDS             complement(39972..41930)
                     /locus_tag="KPH29_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HBG1466057.1"
                     /translation="MRTLSIDIETYSDLDIKKVGVYRYVDSANFEILLFAYAFDNEEV
                     KVIDLVNDEELPKEVIEALNDNKVIKSAFNANFERTAISKFLNINLKPNEWSCTMIKA
                     LTLGLPGSLDSVSKALKFNEDKQKMKEGKALIQYFCKPCKATKVNKGRTRNLPIHDME
                     KWNKFKEYCKQDVVVEREIRNKLSKYKTTEREIKLWYLDQRINDTGIKVDTELIENAI
                     ECDKRYTEKLTKEAIKITGLNNPNSPAQLKKWLSDKVGFEITSLTKESIPEILKQVDD
                     ENVVRILELRKLMSKTSIKKYEAMKLAKGNDNRVRGLLQFYGANRTGRWAGRLVQVQN
                     LPQNHIEDLDLARNLLKEGDFDLIELLYDSVPDVLSQLIRTAFIPSEGHRFIVSDFSA
                     IEARVIAWFAGEKWRLDVFNSHGKIYEASASHMFKVPIESIKKGDPLRQKGKISELAL
                     GYGGSIGALTSMGAIKMGLDEEELQPLVTTWRNANPNITKFWWDVDKAAKKAIKDRTI
                     VKLQHGIKFIYNPGVLFIELPSGRRLSYLRPKIEPHTTFSGDKITYEGMEQTSKQWKR
                     IDTYGPKLVENIVQATARDCLREAMFNVTDAGYSIVMHVHDELVIDVDKKGGSLEEVN
                     SIMGKEISWAKGLPLKADGYECDYYKKD"
     gene            complement(41930..42502)
                     /locus_tag="KPH29_000418"
     CDS             complement(41930..42502)
                     /locus_tag="KPH29_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HBG1466058.1"
                     /translation="MSNSVQSTKVVTGKVRLSYCNIFKSRAMVEGAEPKYSVCILIPK
                     SDKVTLGRIKKAIDAAKEQGKTSKWGGKLPGNLKTPLRDGDAERADEAEEYVGMYFLN
                     ANSTQKPGIVDKDLNEILDNTEVYSGCYGRVSINFFPYNSAGNKGIGCGLQNVQKLAD
                     GEVLGGARASAEADFSDDFEYEDEEEDFLS"
     gene            complement(42525..43097)
                     /locus_tag="KPH29_000419"
     CDS             complement(42525..43097)
                     /locus_tag="KPH29_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HBG1466059.1"
                     /translation="MSNQEITKLVENNLNLVHFSINRFFKSYVEQHPYLYEEFYQEGC
                     IGLYKAALNYDSNRGKFSTVAVSYIRIEMFKALRYGEKHYRNSIEGLDVSVFEDSKRP
                     LKDIHGFYEIQFNYDVDYIRKIARKSYLKDIDKIVDWILQDKKISDIGELLNVSSTVI
                     CGRIKLFSKQVRDLDKFGEITSNIKSIYQI"
     gene            complement(43150..43485)
                     /locus_tag="KPH29_000420"
     CDS             complement(43150..43485)
                     /locus_tag="KPH29_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466060.1"
                     /translation="MPLDAGIKDKLDESISIILDLKDRKKSIEKDLDYEKDNLLDMLK
                     RLNITEYNSTEENAKVIVMDFKRESLIKDKVMSTFLEVNDNHLDRVYIPEHIKVSPVC
                     FVSVRAKDN"
     gene            complement(43500..44666)
                     /locus_tag="KPH29_000421"
     CDS             complement(43500..44666)
                     /locus_tag="KPH29_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HBG1466061.1"
                     /translation="MPLQHARLSASGAHRWLHCTPSIKLEENYPPLTSIYAEEGTVAH
                     ELAEVKLMLEYEKISKKAYNARIKKIQKSEYYNSEMEDYIQSYVENVVELVNDSKAIC
                     DDVIVMLEERLDFSEWVPEGFGTGDVVVISDGILQVIDLKYGKGLEVSAIENPQLRLY
                     GLGAYNQFEMLYDIDLIKTTIIQPRLDNISSEEIEVTKLLTWADNVKKKAQMAFNGEG
                     EFVSGSHCGFCRAKNDCRKRAEDNLKLARKYDFADTFALNKYEIADILGFAKNIQDWL
                     KDVQSYALEQAEKHGVKYPGYKLVEGRSNRKYVDEQEVAKVLLNSDYDEEKIYKPRTL
                     KGISDMEKAIGKKSFAKLLSDLIIKPVGKATLVVESDKRSEINSIDSAKKDFEI"
     gene            complement(44678..45115)
                     /locus_tag="KPH29_000422"
     CDS             complement(44678..45115)
                     /locus_tag="KPH29_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466062.1"
                     /translation="MVEVNVNVKVKVEAPEFTNALLVVANALGGLNLGKAMQIEPINI
                     TDMKEEKKVEVKKAEKIKKVEVKEDVKEEIAEAKEEIEKNNENTTSEVKYTKEEVRTK
                     AAQVSKAGKKDKLKELFGEFGASKLSEVKEEDYSAFMNKLESL"
     gene            complement(45186..45335)
                     /locus_tag="KPH29_000423"
     CDS             complement(45186..45335)
                     /locus_tag="KPH29_000423"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466063.1"
                     /translation="MKLLSLDTNNKNVVTLKKDGKVIADIVFKDIKTGKKISVGILNK
                     KVLVK"
     gene            complement(45316..45708)
                     /locus_tag="KPH29_000424"
     CDS             complement(45316..45708)
                     /locus_tag="KPH29_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1466064.1"
                     /translation="MDISESIAKQFSDSLKSLIEIEISKQETDRVKSQTIEQKVKVLE
                     PKDIVVLIKRGYPNYLITVEEARGILKLDTAFMRRLVSTGLIKSLVRGDGRKISRYEV
                     DDFIERNQGKNLDELLKAVEWGDEIAES"
     gene            complement(45739..45936)
                     /locus_tag="KPH29_000425"
     CDS             complement(45739..45936)
                     /locus_tag="KPH29_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1466065.1"
                     /translation="MLIGDNIKQMLRKRDIKPYKLAKELNIDVSGLYKLLRNKSSNPT
                     IDTLIKLADYLDITLDELVGR"
     gene            46012..46386
                     /locus_tag="KPH29_000426"
     CDS             46012..46386
                     /locus_tag="KPH29_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466066.1"
                     /translation="MQFSNNISKELQESIRKTVSDTSNTEYFYYAEFQYKIILKSIEE
                     FEKELDDEHEIALKLTNFGKDVLMIVEEVGYHNPCLIHYYGIVNGVYSEILQHTSQIN
                     FMITSVKKTDPSKPARRIGFIL"
     gene            complement(46396..46605)
                     /locus_tag="KPH29_000427"
     CDS             complement(46396..46605)
                     /locus_tag="KPH29_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466067.1"
                     /translation="MENKKIEEIEKRITMLENQLQAQLFSQITAQSNSDFIDYMYQCI
                     VLYLHEMNKSEKRKISEITKERIRK"
     gene            complement(46652..47461)
                     /locus_tag="KPH29_000428"
     CDS             complement(46652..47461)
                     /locus_tag="KPH29_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBG1466068.1"
                     /translation="MNNLQIFKNKTFGEIRVIELNGEFWFVGKDIAEQLGYKDTSDAL
                     KRHVDDEDKGVGEIPTPGGNQNMKVINESGLYSLILSSKLPSAKLFKRWVTNEILPSI
                     RSTGTYNMIDLQTKLPTTYKEALQHLIEQVEVNEQLQLESKMKDQVIKELKPKADYTD
                     MILKNKGLVTITQIAKDYGMSGKEMNKILHERGIQYKQSGQWLLYKQHQGKGYTHSET
                     IDITRSNGMPDVKMTTKWTQKGRLFLYDLLKTNNILPDIEKGYSYQTSILG"
     gene            complement(47509..47712)
                     /locus_tag="KPH29_000429"
     CDS             complement(47509..47712)
                     /locus_tag="KPH29_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1466069.1"
                     /translation="MKKRSLKAGRIEAGYTQEELAYKIGIAKSTYNLKENGKRNFTEK
                     EMIMISYILNKTMDELFLEKRLT"
     gene            47889..48230
                     /locus_tag="KPH29_000430"
     CDS             47889..48230
                     /locus_tag="KPH29_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG1466070.1"
                     /translation="MATFGERFKQLREEKKLTQDELVSKFNKVYFTSFNKSTISQYEN
                     NKRKPEINILENWADFFDVSIDYLLGRTLVRNHIDTVAAHRVNPHKDLPEEAQEQLND
                     YIEFLMNKYKK"
     gene            48242..48790
                     /locus_tag="KPH29_000431"
     CDS             48242..48790
                     /locus_tag="KPH29_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1466071.1"
                     /translation="MLILKKLRTEKGISLDKLSTDLNINKSTLSRIENGLREPKESFI
                     KDCSDYFGVSTDYLIGKINIDDSNKLTKCLNSTFPIRLKELRKKKELTQAELSKLLNC
                     SLSKIAMLETSKREPVKEDLLRISEFFNVSVDYLLGKTSIENYITTDEISKIIKSYES
                     LPKEAQEHINSYIEFLVDRYKK"
     gene            48866..49372
                     /locus_tag="KPH29_000432"
     CDS             48866..49372
                     /locus_tag="KPH29_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBG1466072.1"
                     /translation="MNKLDALLDLANNEEIEIYYTDKIADDIKGLYINRQGLKIISLL
                     NSLKQNNAKLIEILAEELGHHFTSVGNYVSSKNSYKNKILIDKTENKALKWACEFLIT
                     EEEIIHVINSHATSVYEIAEELQVSINFLLKRLEFLSKKKSMLDLGNNRFLVLTNLPN
                     FYIYEDIF"
     gene            49992..50861
                     /locus_tag="KPH29_000433"
     CDS             49992..50861
                     /locus_tag="KPH29_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HBG1466073.1"
                     /translation="MIKNFDEWKEYDGFSYGSGRSEKIWLINEKTKDIGLFKFPKTKE
                     TGEYWAEKLACEIASLLKIPCANVEIGTYNKRIGSMSYLINKNDEKLIEGVWLINKYY
                     PCYNTDKLYDYKNNEYYSLKMIFKSIEEYNIKNDFLKMLIFDYLIGNRDRHQNNWALL
                     ESQNKITLSPLYDNGSSLCCYVNIENIDSFFTDHQRFEALINTRSRSRIRIDGKNKKE
                     PTHLEVLKYLNSNYYEQTIDFVEKIINLMNEVNINTLIDNYSENIISPKLKKLLKIYL
                     KEKVLRIKNIYNL"
     gene            50874..51581
                     /locus_tag="KPH29_000434"
     CDS             50874..51581
                     /locus_tag="KPH29_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HBG1466074.1"
                     /translation="MSIKNGKDFLYVIWKDPKSRRQFIIGKLAKNGLYEFSYEYEIDE
                     AIKAGFNALISFDDISKTYYSEDLFSPFASRLPDKKRKDINKILDKYDMSEYDTYELL
                     KRSGARLPIDTLEFVDPIPLDIDKNIIRYFYIAGVRHYAGCEGHECDNSIELESNEAL
                     VLELEPENKFDPHAIKVFDKKENHIGYIPRYYSGELTSIINRGLKYNCIIKFVNKENN
                     CDECIKVKLEILFDEYN"
     gene            51660..52790
                     /locus_tag="KPH29_000435"
     CDS             51660..52790
                     /locus_tag="KPH29_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG1466075.1"
                     /translation="MKGGVRKRSNKWYYYFDLGIVEGKRKKVERVGGNTKKEAEKALR
                     EALNEYENSGIVFEESNISLSDYLDFWYKEYVLLNCKYNTQESYRINIEKHIKPKLGA
                     YKVKALTPAILQNFINKKYKEDYSQNTLQVLKAILHRSLKSAVHPYKHIRENPMQYVS
                     IPKTKSKTETNKVKTITLEEFNQILNIFPQDSFQRIVLLIGFHTGMRRGEIIALKWDN
                     IDLDNKTITVKHTLIKKPNGMFELGQPKTESSCRTIFTGDTLIKALKEHKLYQKKMKL
                     KYGEFYFDSDWVCTKENGQQVNTHTLDTIVRQIRVALNNDFHFHSLRHAHATLLLENG
                     ANIKDIQNRLGHSQLSTTMDTYSHVTDKMKNETVDIFEKITN"
     gene            complement(53148..54836)
                     /gene="ilvB"
                     /locus_tag="KPH29_000436"
     CDS             complement(53148..54836)
                     /gene="ilvB"
                     /locus_tag="KPH29_000436"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HBG1466076.1"
                     /translation="MNGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPVIYA
                     GGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLIERVESKNNSNWKEEIKTFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWSAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKAGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVSNMEELGKVLDVIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            complement(54889..55884)
                     /gene="ilvC"
                     /locus_tag="KPH29_000437"
     CDS             complement(54889..55884)
                     /gene="ilvC"
                     /locus_tag="KPH29_000437"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBG1466077.1"
                     /translation="MKMARMYYEKDVDLEVLKNKKVAVLGYGSQGHAHAQNLRDNGVH
                     VVIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPDEKQKKVYEESVKDNLKE
                     GQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVFTKGSGVPALFAVYQDYT
                     KKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVLCGGISELIKLGYKTLVD
                     AGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEYGDYVSGKRVITDAAKQG
                     MQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIAEVGRNLRSMMSFLK"
     gene            complement(56154..56393)
                     /locus_tag="KPH29_000438"
     CDS             complement(56154..56393)
                     /locus_tag="KPH29_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBG1466078.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
     misc_binding    complement(56506..56763)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(56924..57685)
                     /locus_tag="KPH29_000439"
     CDS             complement(56924..57685)
                     /locus_tag="KPH29_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBG1466079.1"
                     /translation="MNTGSKDIDTLNVNFRGIEIKAVLVYKNRKNISIKIDPFGKIIV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKYKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLKDSVKKSNCRITINEYQIVVQTNDTSTDFIKKSLKSWYKMESERTVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLV
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     gene            complement(57690..58511)
                     /locus_tag="KPH29_000440"
     CDS             complement(57690..58511)
                     /locus_tag="KPH29_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HBG1466080.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANYER
                     FPESEVVVLDSFENIPDFPTDENSYIIILTRGHLYDSSALEWALKRETGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            complement(58553..59308)
                     /locus_tag="KPH29_000441"
     CDS             complement(58553..59308)
                     /locus_tag="KPH29_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBG1466081.1"
                     /translation="MTTYKKLKKSIFKISIFSICLLVSLVDINPIFAGSSSNETSNSD
                     TIKVLNAKSLNFKQGNYEVNVSIPKIKGLKDKTLENKLNNEFLQDGKKIYSEFQNKMK
                     KENNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEDSSSITKKHYTVDKNRGILLNLPI
                     FFKDSRYIRAISDNIKEQMRNQIKKDDTKSYFIDQDKDFPVKDFESINKYQDFYFNKN
                     GNLVISFEEYEVVPGYMGAVEFVIPNKVIKRLR"
     gene            complement(59351..60220)
                     /locus_tag="KPH29_000442"
     CDS             complement(59351..60220)
                     /locus_tag="KPH29_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBG1466082.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIGVIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKAHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            complement(60232..60714)
                     /locus_tag="KPH29_000443"
     CDS             complement(60232..60714)
                     /locus_tag="KPH29_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG1466083.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            complement(60766..61698)
                     /locus_tag="KPH29_000444"
     CDS             complement(60766..61698)
                     /locus_tag="KPH29_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG1466084.1"
                     /translation="MVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVLSATNNYIN
                     GQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVDSKYQELED
                     KMNLNTSYKEKMDKAGVDKEYLKQEISRDLAIDKNKKAFEDRINISDNDMEAYYTSHK
                     KDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESFESLAKKYS
                     DDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVKVTDKRERQ
                     KSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            complement(61791..62624)
                     /locus_tag="KPH29_000445"
     CDS             complement(61791..62624)
                     /locus_tag="KPH29_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBG1466085.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDEMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
BASE COUNT        19355 a        11378 c         6597 g        25466 t
ORIGIN      
        1 gtttaatatt ttatttccac taatatctag atattttaat tttatatttt tacttaaatc
       61 tacactttct agattatttg aactacaatc aagcttttct agatttctaa aattttctat
      121 cccatctaaa ttatctattt tgtttggatt agaaacatct ggatttttat gtaaatctaa
      181 ctctaagcaa ctctcaattt tatttaaatc actttcatat attttatagt tatcttttga
      241 tgcaacttca tttgttaatg gatcatccct attatctaat accttttcaa caatatctct
      301 tctaaaggat tcatcaacaa atttattgct tatatcaatt tcctttacat tctttctttt
      361 ataagatttt atgttattgt tactctctaa atctaatgca tttacaaata atattgattt
      421 gttcattatc aatgtaacac taacaactat cagtaatgat tttatatact tcttaaatat
      481 cataatctcc ccccatacct attaataata aataatattt atctaaatat taccatactt
      541 atgattttca agaaatacta aaatatttta tataatctat ctattaataa aatctaatgc
      601 cttataaagt gtatcaaatc tatcattacc ctttatcata gtaaattttt ctttagtcat
      661 agaaccaatc ttctcacatg ctgcaccacc aatgacataa agattttgcg tctgacctgg
      721 cacgtaatct tttatatcac atatcagtat tttcccatca ttataacccc aaccaactac
      781 agttgcagag attttgtcaa cttctccatc ataaacaatt gtatgtttgt acatctgttt
      841 aactccctca ttatttatat ttttatttaa tacaccttct acaattaact tagcaatacc
      901 ttcatgacct agtttcttag ctttctcata atcttcttta ttatcacaga agaaactttc
      961 aattaatact gctgtaggat ttgaactatt taagatatat aatcctttat ctaatttagc
     1021 tcttctattt ctaaatactg tacctagctt attacatatt ctagttgcat actctaaacc
     1081 tttattacta taatatagaa cttctgaacc ttttccttga ccatcacttg catttagatg
     1141 tagttctata agtaaatcat atcctccact attaactcta ggtattttat aagtcttttc
     1201 ttctgcttta gttttaaact gcttttcagg acatattatt acatctgcct tatgaccctc
     1261 ttttctaaat gtatctgcta atactggtgc aagagattta ttgtattggt attcgttaac
     1321 tactccatca gcagaagtgc atgcaccact ttttaaaata ctgtgtccta ctgtaataca
     1381 tattttcatt atttattttc ctccttcttt tcaataaaaa aacacttaca tatagtaagt
     1441 gtttataaac ttcttaaagt aactatttta tttagccaat atctctatcc aattattagg
     1501 aaaaccaata agctttaagt ctatagaatt attataatca ttaattaact tttgaagttt
     1561 tataaaaaaa ctattccaaa ttaatttatc catagtcaaa tgcttcatag caagtatata
     1621 agaaaatatc tttttattat ccaagttata ctttttatat tcgtttttta ttgttataat
     1681 acggaaatta ttattatata ttctaccata atgagcgcat tgatttctga cctgtgttaa
     1741 tgattgtaac caagatttaa ctaataaagt attcactcta cacaaattat tttttatgta
     1801 tctagtatct tctggcaaca tgtttgaata taattttgac aacataccaa aagtcattat
     1861 ttccgttgca acccaaatgg gaagttttcc ttcatatttt tctttatggt gtataataaa
     1921 aagcttatct gaattattac ttttttctct ctctaatgct gtcaaaaaat taatatagaa
     1981 tttttcatct ttgaaactct ccctttctag atatccacaa gcaccatgtt taattgctaa
     2041 tgtatatgca atgtatgtcc taaatgctat ttctatgctc cctaacaaat ctgttaataa
     2101 tatcctaaat tccttattaa acctatatat atcataaact tcttcaaacg tagttccttc
     2161 tttatatgag ccatcatcat tcttaaaact tagaagatat gctgtaaacc tataataatt
     2221 gacattactt aatacaaatt tagcatcttc tttatcattt attattaatc ctctactttt
     2281 taaaatatct atttgttcat caaatgtttt ttcttctttt acttcaacca ttgttttcac
     2341 cttttcatag atatactttt atataataaa acccaccaat taatattagt gggtaaattt
     2401 atctgtcccg cctatttgag ctataaaaga agcttggcgg gttccttcaa aaatcaatgt
     2461 cccgtatatt tgagcatata aatgctttac gagttctgta ctattattat atacttatta
     2521 gctataaaag tatactctat ttaatgaaat tttttaaatt tttgtgagta ataatatttt
     2581 tgttaatttc attacttttc accatccttc aactgtttgt aagtttgatt tatacctatt
     2641 gcaactcccc aacaaattac accttgtaag actgcaacag gatttaatcc tagcatccat
     2701 attgagaaac ctattccaag tatcagtaac actactggaa tgtatttatt atctaattgt
     2761 ttatattttt tacaaccttt acctataata gagagagcag ctactaaaat tagcaactgc
     2821 tctggtataa aacttattaa attatccatc tcttattttc ctccaattct atttacaatt
     2881 tcatcaattc taagatgtgc ttgttttgta cttgcttcta tttttgcaac tcgttcacta
     2941 atatctaaga aactagtatt aaactttgct acatcatgct ttatatctct tacattctca
     3001 cataaaaaag ttatttgttg ttctaatttt gtagttgttt ctgtttcatc ttgtatcttt
     3061 ctgtttgagt tatttctaaa tgctaagtag gatattacaa ctcctgctac tgcacatagt
     3121 agatttatgc taatttcttc catacatcct cctagtttta ataatataat ttattatgcg
     3181 aaacaagcaa tataataacc tgtaaaagga tatgactcat aactagtatc atatgaatga
     3241 actgaaaaac cagttttatt gctgtttata gtaatccatg cacgttcaga acctcctgta
     3301 ttacttccta aagaactacc actgggatta taataatata cacttcttct aaagtaaaca
     3361 tagacaataa ggtaacttgc actcttgcca aaattagcag aaaaaggatt accatcatat
     3421 ccatcaatag gaccatatct aatagaatat ttgcttttca attgagtttc taaattgctt
     3481 atattgctct taaatcctgt aatatagttc gcataatctt gaaatgtttg ttctgatgta
     3541 gtaggagagc cgatagcagt tgataaaagt gtttttccac tatcggcttc ttgaaaagcc
     3601 tcatctgctc tatctataca ttctttcaat gctccttcta cattttcact tgtaaattta
     3661 ctttctgtat cttctatagt tacattcttt gcttctaata caagatttct aactttatta
     3721 accaactctt taaaagtcat ttagtcacct tctttcaata aaaaaagaac cctctatata
     3781 gttggttctg ctccttctac tactccacta ttttttataa tataatcctc tacagctttt
     3841 ctgtactctg tgttagtcac gtcatctaac tgaaattctc tattttttag aggatttaat
     3901 cccttactta atattctttc tgctaatatt cttactacaa cattatttat attcattata
     3961 ataatcctcc tactttctca ttttctgcaa ttaataattg attttctaac tcttgtattc
     4021 tcttttcctc atctgttata tatactggta tttcttctaa aatcggttct tttgtttcta
     4081 tatttatacc tataactcta ttccttgtat aatctatact tccatattca atatctatat
     4141 aatgcaattc cgttattgta taatgtggca atacatctcc acaggattcg cctgtttcaa
     4201 agattgttct acctgtttgg tcatatatta ttcggtttgg aacattcatg ttttttcctc
     4261 cttttaaata aatttaatag cataccattt ataagtatgt ttttgagttg aatcaaagtt
     4321 ataagctgga atctgaacgc cagtattatt aaaatagaca tccccctctg agttactata
     4381 tagccatcca ttagcatgaa aatactcatc accacttttt ctgataaaag atgtaacact
     4441 tgaaaaatca acacctcttt gtgtggcaac accattacaa gcgaaaagga aaagtttata
     4501 attattttta tattcagcat catagtaatc aaaatcagca aagaaaatat taggtttaaa
     4561 gcctaaatta ctaactttaa tccaagaacc aggtttagta acattgtcgg ttagatatgg
     4621 ataagcaatt ttagtactat ccgcaaaaga agtaactgta ccactagcaa ctttatactt
     4681 agtatttaac tgcgatatag tattattagc ttgtgttaat tgattcatca aatcttgtaa
     4741 actagcgtct ccactatcaa aacctccctt aattttctct gataactcaa cgagtgtatt
     4801 atttaaactt gcatctatat tctttaatgc taaagtattt ataatgcttg ttttaccatt
     4861 tctaacgcct tgcccaatct ctgttaactt agttgatata tcttgtaatg ttgcatcagg
     4921 ttgcaatggc attatattct tacttatact tataactttt tcttctacaa cattttctgc
     4981 atcagttaca actattttta atgtgtgtat tgcattgtct ataagttcat agtttatagt
     5041 cttttctgaa tacaagtctg tagtaaaagt ctcttttaat gtttcatcta aataacactc
     5101 tatttttgtc aattcactgt atatttttgt tacagtaaac ttagcttggc tatcagttac
     5161 taatgatgtt gatatatcaa ttcccttttc tattttttta aaagtaaaaa ttctatcaaa
     5221 attatcatat ccatcattta attgtatttt tagtttgtga tttccatcta cggataaaga
     5281 gtttagttga ctatctgtta ctgttgctgt taaagttgaa ttttgtacaa caggagtaat
     5341 gttttgtatt aatttatcat caagataagc acgtactatt aaattaacat ttggagtatc
     5401 atcacttaca gtaaaatcta cactaaatcc ttttctttta ttcccaagtg aagtatctga
     5461 accagatatt acaggtctac cttttatagt tattattcta ttagaagtat acggaaattt
     5521 ttgttgtgta tccacatgca ttgattcaca attataagta aatgtatatt gaccatattc
     5581 ccccaagacc caaacatttc ctgaacccca atttaatgtt gaaccattgt aagtgcaact
     5641 taagcaagtt tctttaaact caccatttag tccatggtca ccaccagtcc actcaaaatt
     5701 ttgccttcca cctgcaaatg gattaaatgc aagattttta gtatattttc ttttgtcagg
     5761 taaattaaaa taactcattg ataacgccat tttttcacgc tcctttctat ataggtagta
     5821 tgtcgttatt tacagtcgat ataatattac tattaattcc tgtcagttgc tctgctacct
     5881 taccaatttc ttcatgtatc ttttttgatg aataagtagt catttcagac actctattat
     5941 catctacagt cgcattaata aaatgagtct ctgcattccc attaatcaca taaacattta
     6001 actcaacttt tactccactt ctaacctcaa tagaattatc gtctataatt gtgaaatcgg
     6061 gtactacatt atcttttgta gtagcatcta taatacttac agttattctc tgtgttaata
     6121 aaccatgtgt tacagttgct ttgaatccac cttcttgatt atctatccaa tcatcaattg
     6181 ttattgtttg agtagatgca acatttgaac cacctgctat tagctggtca attttaatat
     6241 tttgtttctc attttctgtg tcaattctag tgtttaactc tgtcttagca gtttctatgt
     6301 tgcttgttaa ctctgtttta gttgtatcaa ttttagtgtt tacattgctt attttagcat
     6361 ctaaatcctg tatatctttg agcgtagcaa aaattattgt agggtcaatt ttaagttcta
     6421 tattatttat attagataca ataagcacag ttttaacctt catatcaaca actgctcctt
     6481 gttctataga aggtttataa cattctttat atttagagat tgcaattaga ttattttcat
     6541 catctaaata tcctatttct cttatcataa atccacctac acttgatggt attaaactct
     6601 ctaatattat gcagtttggc gcagtttcat ctgtggttgt atttccgatg ttgccttccc
     6661 ataccacatt tttaagttct gtctgacttt cagtaggagt atactcactt cctccaccat
     6721 caccaagttg aatttttaca aaccctactt tattacctgt gacacttgca tttgctatct
     6781 ttgcttttcc tacatctgta attatagtgt aataactttt atctatagcc aatatatcac
     6841 ctcctaaaat attgttatct cttggtatcc aactccattg ccagttaata catcaatttc
     6901 tccataagtt tctatatctg gtggactcca agggtatata gttatttctt gacccattag
     6961 ggttgttata ccaaaattca tataattgtc tttgcttata agcactctag tgtaatctaa
     7021 agtcatatta catggcttaa tattacttac aaaagaatga acttcctcaa accaatcttg
     7081 atttctagca tcactctcaa ggtgtatgtt ataagtagca ttattaatag ttaattcata
     7141 attgccttct ccaactacat tatctagcca gttccttaaa aatctctctg agtaaggtaa
     7201 tttacttata tatttactaa aaatcctaaa ccttctatct tctaaactct cattactttt
     7261 aggagttata gacattatct tttcccatct ttttatacca cttatagtta ggtcctctaa
     7321 aaactggtca tttgataggt cctttaattt atcatgtagt gtttttattt ctttattttc
     7381 tacattaaat acttttatat attcttcttt atcttgtaga atttgtggca agtaatttat
     7441 tagattaatc tctttatcca actacctcac ctctcactac tatgctgtta ctatctattg
     7501 ttagattaga tttaacatca tttatcattg tatttgcaat gtctaatact ccatcaatac
     7561 taagtaatct agtttcaatt tgagatatac ggactattaa gttttcttca tcttcccaac
     7621 tcatgttaag ttcatttaaa tagtcgtcta ttgcttcttc tgcaattgat tttatattct
     7681 cccaagtgta gccatttttg tatgttattt ctgctgatat gtttatagtt gtacttgtaa
     7741 ctccttcaac tgtgactcgg tgtccaatcg gtgctaatcc taagccttct ccttggtgtc
     7801 caattgggtc aatttcttct tgaactaagt ttactaattc ttcacttggc actttaaaat
     7861 tagagttaat tattactaac ttaacagtcc cacctccatc ccaagttgga taaactttaa
     7921 ctcctccaac atcttgtatt ttgttaactt catccctata gttttgtata ttcccgccaa
     7981 aactctgtga atttaggcta tcataatatc tttgtcttaa actatcttct gactcttcat
     8041 cctctccatt tatcaagatt tcagttagtt cagcagtttc aagaccatct atatattcaa
     8101 taggtattag ttttcctaac tcaaatacag gtccagtagt ttcacatttc attttatatg
     8161 ttttttcaga tattctctca attgctacat agttatattc tcctagatta aacctagaat
     8221 caagtggaat atctatgtta aaaactcctt tagcaatcgt atgagtggct tcaagtggtg
     8281 ttattcccct ttctttacat ctcttctcta aataataata actagcagta tctacgaatg
     8341 tttggtctag taattcatcc atagcaatgt atgtttctgt aagttctata gcaacaggag
     8401 caagagcatt atatattata gaaccttccc ttttatcaaa agtatctggt acactatcta
     8461 acattctttt aattatattt tcaaatgtca ttaactcaaa caattataca ctcaccacct
     8521 tctctgcttt tatatttcca tattttgtat gaactgaaaa tctacattgt actttaccct
     8581 ttatattttg aaactcaaaa ttatctatat tttcaatcct atcatcttga attagtgctt
     8641 ctgttatcct tctttcaagt tcgggtatta catatgaaat aggttctcca attaaatcat
     8701 ttaattcaac accataattt ctagaataaa taagatgctc atacctttca gtatttaaaa
     8761 ttaaaaaaat ggtttgtttt aatgcttcta catcatcaca aataccatca atcttagact
     8821 tttctatatg aagtttaaat gtcttacttg gctcttgtct tacatcaaaa ttaattattg
     8881 atacatcttc aatatcataa tctaaattat cacttggtaa cactttatca catcctatct
     8941 aaaatcaagt attgttgccc tccttgcata cgaattaaga ctaatttatc tcccattttt
     9001 ttatctgtat atcttttaaa tgtatctgtt tgtattagaa aaatttcacc aatagatagt
     9061 ttttgttcta tcttaactct tagaggacta atactttcta ttgttccaaa tacaaccctc
     9121 attgggttgc ttgtttctac tgcatccatt gcagcttttt ttattatctg taataaatct
     9181 tggctcatat tgccacctca cttatataaa tcttctcaca tgtgtgtatg cttttccttt
     9241 tctataagaa ttaacagact ctatttttac cacatctcct gtttgtggtg aatgaattat
     9301 ttgattgttt ccaatgtaca ttacaacatg attactactt cctccaccaa ttctacataa
     9361 taagtctcct gctttccact tgcttctatc ttttaaatct actgcttttc ctgctttact
     9421 ttgtgcagaa gcagtacgag gaatttttat acctatttgt ttataacacc attgagtaaa
     9481 cccactgcaa tcaaaattat taggaccttc tgctccatac acataattac aacccagttt
     9541 actttttgct atactaatta atttatcttc tttagaattg ttatttgtat tactttggtt
     9601 attaccttca acttgatatg tttgttcttc atctccacct ataattatat agccattctt
     9661 tctaccaaat tttttacatt cactagcatt agctaatagt atatctatat gatatgttcc
     9721 gtttgtttca acatatattc ttcctccatt atctttaact gtatatactt tgttgtcata
     9781 ggcagtacca ggaagtataa tttttacttt atctccatat tcaaaaactg gatgtttatt
     9841 tagaaaatca tcagtatacc aagttttctt aactccttct cgattcattg gaccagcaac
     9901 agttcttgat tttacatcaa gtggctttcc attgcaatct gtttttccac cttccattgc
     9961 attgtttcct ggataatatg cagtaaatat agcaggaact tttttacctg tatttttttt
    10021 cataatactt tgtgcaggac catttttctt ttcatcttta ttattagtat ttccactact
    10081 atatgagctt gaagaatatg aagcaaattc gtctccatca accaaggtca aatccatgaa
    10141 atgtgaatta ttttcaaatg tatgttttac tttctcaact aacatataat tttgcaattc
    10201 aatatctcct agatttaaaa aaacaggtac taaacaacct gctctcactc taatatctcc
    10261 aagtgcattt tttaaactta atgacttagt tttcttatta tatagtttta gaagtatatc
    10321 acacttttgt tttatctctg cttcactcat gtttttgtct actgtatcaa acatttgaag
    10381 tattccccaa cttctcatgt gagctgagtc ttgagcaata tatacatccc tttttcctga
    10441 ctcctcgttg tctcttacaa gtttaatctt tgtgtaagta tcactatcta ttgatgaatt
    10501 atagtcaaaa tcctcaatta catcattgtt catgacagta tctagtttca ttgatgcaac
    10561 attctttaat gttattcttc caaaatcatc atacaaggta tacatttctt ttttctctct
    10621 taaagtatca tctagtgctg ttagtatcat gtcaaagagt gttttatttt cttcgactct
    10681 agatatttta tacttagtat cttctatgac attgtatttt aaattaaaat ctttagccaa
    10741 gatttttaca agttcacttg cagttttatt actatataca taagtatctt tattcttaaa
    10801 atatctcaac tggtcgtaag caacaatttt aatgtgattt tctttatctc ttttcttttg
    10861 aaatatatat ccataaaata tgcctattcc tttataatac agccttacag aatttccttc
    10921 gcaaaactct agtatatcat ccatgactat tgtaaactct aacttagaag gtgtacctcg
    10981 tctttctatc tcccatgtga taccatcaat gacagcaggt tcgtagaaat cttcccaatg
    11041 agctattact aatcttacat ctctatcatt tgctaacact aattcatcaa ccaagtttta
    11101 acacctgccc tttataaata gtgtatttac ttaagttttt gcccttattt gccttatcca
    11161 tcatagattt atttagttcg tatactttct tatataatga accattacca agttgtttct
    11221 gacaaattga ccaaaggcta tcccctgctt ttactgtata tgttttagtg tttgtggcat
    11281 tgactgaatc aactcgtttt ggctctatct ttacattagg tctaccagtc tcatttttag
    11341 gaggggcaag aactaacttt ttagttgagt aatctctata ttgctttaat tttattgcaa
    11401 cttttgtatc tgagccatta tctgcatctt ctgaaatagc atactcttca agagatactt
    11461 ttatattagt gttaaataag accttgttac ctaactcacg agatacaata aattgaaatg
    11521 gcttacaatc agtttttagt aattctagct tacttaaaaa gaattgaaca tctctaaatt
    11581 gacctctaca aaaaggtagt ttattatgtg taaattctgc ttcaaaactt atttcagata
    11641 atccttcttt ttttaatatg tttacttctc cagtatttat caaatcaact gtcttgttct
    11701 tgtttgtcac tttgacttca agcttgggtg gtgcgattgg tagttgtact ccatctaggt
    11761 aaaaatcata agccattttc tcactctcct tcctaaacta ttccttccgc tgatacaacc
    11821 atagcatcgt tcagtttttc agttaagaca ttaactattc catcaacatc tgtatcttta
    11881 cttatgttgt ttgtattgtt catatctatt ttaatgttta ctcctgtgaa tcgattgatt
    11941 gtttcttgct ctgctatatc tcttaagtat tttaagtcct cttgactttt atccatagtc
    12001 tttgccatct ttgcagtatt gcctgccgtg tcctttacat tttttcctaa atctccacca
    12061 aatccaccaa ggttgtttaa aggattttgt ttattccaca aatcgtctaa tcctaatttt
    12121 ttctttgcat cttctagcat cttattaatg tcaaaagtat ctttaaattt atcagttata
    12181 gcattttgcc atttagttcc aagtgcattt cctttttgaa atgctgctcc aatatctttg
    12241 tatcccattc gctccaattt tactttctct ggtgcgtctc ctacccatct gtttaagcta
    12301 tcaatctgtt gtttgatgta actattatct gccttaactg gcgtaaacgt tgcctctcct
    12361 actttaccaa tatttatccc tggtatttta tttattaggt caattaattt gtttacacct
    12421 cgtattgcga tatttgcccc atctatgaaa gctttcccaa gtgcatttcc agcattattt
    12481 acagaattat ttagagatgc cattttctcg attataaaga ttacaccttt tgcaatagct
    12541 tgtttcatta gatatacaca ttgattccaa ccatttgtaa tgccttcatt tacagctata
    12601 cagccattta ataaccaaat cataacattt tgtattgctg ccactgctgc aaatacagca
    12661 cccacaatta ctcctaaggc agttaaactt gtacctgcga atttgtttac tgctgctact
    12721 gcaacaaaaa ctactactac aaatgctaga atacctaata ctacccatgt gattggacaa
    12781 gctaataaag ctgcatttaa accccaagtg gctattgttg ctgataaagt agctccattc
    12841 gaacgcatca gtgctgctgc ttgtatatta gtcataagtg ctgatagtga agaggaagca
    12901 gcgtgagcaa cattcgcaat tacgttacct attgtagcta ttttagtcgc tagcaaaacc
    12961 cctctaaaca atataaaaac agaagctaca ccaaatacaa taggttttat tatactccaa
    13021 ttttgtgcaa atatattaac tatatttaat gcagtattta caatagaacc taagctttta
    13081 gttattacta ccgcacaaaa tgtaaatcct tgcactaagt ttgaaaagaa ttcactattt
    13141 aaaatatttt ttataatatt taatgtatcg taaatgtttg aagatattgc aatcataaca
    13201 tttcttatag atacaacaat ttcactaaat ctaacgcttt gcaatgctcc acttatcttt
    13261 ccgagggttt gcccaaaaat catgtaggcg tcatttttca tcatagtaag tgcttgacta
    13321 aaagtgactg gcatacttgc gaatttcttt tctatctcat cagaagcttt aaatagtgca
    13381 tttcttataa catctgctgt tattgctcca ctacttgaca attcctttaa ttggtcttta
    13441 gtttttccca ttgcatcagc tattttagtg gctaataaag gtgcattttc cattatagag
    13501 cgaaattcat caccttgaag tttccctgca cccatagctt gagtaagctg atacatagcc
    13561 gcactagctt catttgctga tgttcctcca attacaaaag ctttattcat aagttctgta
    13621 aactttatca cctctgccga acttccaaaa gcatctcctg ctaatattcc aagtttagcg
    13681 acttgtgctg ccgtatctgc ataactagct cttgcacttt gagcagataa ataaatcatc
    13741 ttattaagtt gttctgttgt ctgtaatcca tcattcatta aatttaaacg tgcttttgta
    13801 cttgcaattg tatctgctgc ttttgtaata ctttctatac ctcttattcc aatatatatg
    13861 ccagccaaac ttttgagttt agatactaat agagaacctg cttcacttcc ttgctttatc
    13921 ttattattaa agttttcttg ttcatttgta tttcttccaa ttttctgttc tatcctggtt
    13981 aatatgtttt caatattgtt taaactttgt tgagatgctt gtatcccacc tgtgttaagc
    14041 ggattatgca atctttgttg taacctttca aggctattaa ttgtcgtatt aatagatgta
    14101 gtcatattcc taaaagcagg tgtcattccg tcgaaaatct ttattgaagt ttgtatcgtt
    14161 gccactttct cactctcctt tcataaattt ccatataaaa aaacacctac attagtaagt
    14221 gtttattata ttacaaattc aataattctt ttttcttagt attaaattca tcttgtgtaa
    14281 tagcttccat atccaaaaga ttcttatatt ttaatatctc atctgctaca gatttagtat
    14341 ttatattgtc tgtctcttta atgctttgtg taatagcaga taatgttgat aaaattttat
    14401 gggcatcttt gtaagctttc tcataagcac tagaattagt ttttgttttt ttattaatta
    14461 attcgatata ctcaatagga ttatcaatat tattaattgt tattttaatt ttaaatgtat
    14521 caacaacttt tcttgttgtt tttttacctg ttatccctcc aactatagct cctgcttctc
    14581 caaataaaat tcctcccgct aaagctcttc ctattcctcc tttagttaca gtttcaccat
    14641 tttcaagaag ttcaaatttc acgatatctt cataagaatg aatgatagga gtttttttat
    14701 cgtttatact ttttttagac acaagccatt ttttattatc ttcatcaaat tctatatagt
    14761 tttctatatt attagttgta ttaaatattt tttgaagttc attatttttt ttagttgatg
    14821 atatagtctt ttcaatatct aattttctta aaattttagg tgctttagtc atagaaagtg
    14881 catcacaata ttctttaaaa caagaattgc atatccaacc atcttcaata ttcagtactc
    14941 ctatctcagt attacaaata caacatatat ctgctttttt ctttccaaat aaccccataa
    15001 gattcccccc agtacaataa ttttataatg taattatact atataagtaa aatttttaca
    15061 ttattatcac ctccttttaa ttaaaaaagc acttacttat ttaaagtaaa tgcttcatat
    15121 ttttaaatct atatattatt tttcttttaa tataaattct tgctctgaac cactatattt
    15181 tagtttacaa tcagaaacta gtaaatcctt aggaatttca aatgcaatat tacctgttac
    15241 atctaaatta ggatttacag tatccatagt tatatattta tttcctacat caatcaataa
    15301 agatggaaca tattttgaat catctggtcc tattaatgtg aatgaattta gcataaaaga
    15361 ttggctttcc ttagtttgat tttttattgt taaatttata ataccatatt tgccactatc
    15421 tggagtgtat gctaaataac cagatgctac tgatatttca ctagcttttg aaatgctatt
    15481 aacagttaat tttaaatctc ctatactacc ttcttcacca atatatttag ttaaatcctc
    15541 tttctgtttt tcagtgcttt cattattagt actgttccct gctattgctc caacaactcc
    15601 agaaaataaa ataataagaa taaaccaaaa tagacatcct cttcttctct ttccttctct
    15661 cataatatcc ccctaaatta tattttataa gaatattata ctatatcttc aaaattttta
    15721 cattataatc acttcctttc aataaaaaaa cacctacatt agtaagtgtt ttttaattat
    15781 ttttaatttt aagtccacat agttaatata aaaccttctg atttagcaaa taaagctgta
    15841 aataagtctt ttacatacca catagttaat ataaaacact gatgttacag attcgttgta
    15901 tgtcttggga gctgtattta cattccatat agttaatata aaacgtgatg ctatgtgtat
    15961 aaagacaact actaatgttg atttacattc catatagtta atataaaact tgatgtttca
    16021 atcttaaata cactaccatt acaaagattt acattccata tagttaatat aaaaccccaa
    16081 aataaattgt gtatttccaa gacttacaca catataactt tctcaaattt gcagtgagcg
    16141 ggtaatagtg caattgataa cacttatcaa accttctcaa tgccttgtat tccaattgtt
    16201 aaactatact tgagtagaaa aatcgaacac tgcaaaattc ctatatttta ttataccatt
    16261 ttttaccata taaagcactt gaaacagtga aatatccaag tgctttatct atttatttaa
    16321 ttatttttct ctaatttttt ctaagaaagt ttatatacta tactttctat cttattatat
    16381 atatctatat ctgtttctct tattttcaaa agtttaattc taaattcttc ttttctcttt
    16441 tcaatatcta tactatcaag taaattctca tgcattgtat taatcccccc aaaactaaac
    16501 taaattattt aatacaatct tataaattta ctcaatctta taaaccacat gataattctt
    16561 cttttcacct gcaatcttag taggtctatt attttcctct atccaatttc taaccttatc
    16621 tattacactc tttgtatatt tatttacagt accagtccaa gaaccattag tttcccaaac
    16681 gcctttgact tcgttttctt ctaaatcaat ctttttaata atttcacaaa cagccaactg
    16741 tgctggttta ttactcttag aatatatttt cagtttagat gctatttgct ttgtatcaaa
    16801 ataatgttct tcttcgttta tctctattgg taaatcaatt cctgctttct tatataatgt
    16861 tttagctgtt aatagtttgg atttattgtc aaagcctgca ccatctaata gttcttttaa
    16921 catagatgta ctattgtaag ctaactgtaa tttctcaatc tcacttgctt tttctcttag
    16981 tttttcggga ttagcattgt tagttatgta tgcaccagtt tgtcttatgc ttggtaaaac
    17041 ttctcgtcta agccatttcc taaactgtac accaataggt ttgtctgtgt attgtaaaaa
    17101 tccatataaa ccatcttcat aaaaaatagt tatacttcta gctttattac taattatatt
    17161 atttgcgact acatttaaag tagttacaaa atcattaaat tcatttcctt tcaatacatc
    17221 atactcttgt tcaatctcaa agtcttctgc ttttatgcaa tcctgtattg ttttagatac
    17281 atcagcataa tcgaataatc caactatttg attagctatc caacaagatt tttctttcca
    17341 cataaaagta taaatttgac ttccattgaa ctcttttact attaaatttt tcataactat
    17401 tacactcctt aattgaattt tttcaaggaa tgacgtatac tatagttagt gtatataata
    17461 tacgtcaata agggatgttc aatctttggt cggggagaac gtctcttatt ttttattcct
    17521 ctttttctag ctcttcatta atcttttctt caagccaaat agttttagtc ttgttctgct
    17581 tttttaaatg ttcttcaatt ttctctactt tctctctatc tagtagaaca ctaaaagttt
    17641 ttttattctg tcgtctctgc ttgaagtaat ctgctctact gctatcagta ataattttca
    17701 cctcttttct gtatcgcgat aatataatta tacattgtat cgcgacactt ttcaagagtt
    17761 ttttaacatt tttttctaat tattttactc aaccgaccaa cttgagcaaa atcaatcact
    17821 ttagtgcatt tttatattgt ataattctaa aaagttgtac ttagcaactt tataattaag
    17881 taatttgagc aaaacaaaag cacctactat ttaagtaagt gctttctttt ttctatttaa
    17941 ttttgaatcc acatagttaa tataaaacat tcaagaagta actgtaattg ttcatctgta
    18001 aaataattta cattccatat agttaatcta aaacgataga caaagctagt gcagacgcta
    18061 aagtacaatc atttacattc catatagtta atctaaaacc ccaaaataag ttgagtattt
    18121 tcaataccta cacatataca actctcttaa atttgcagtg aaccatgagt agtgcaattg
    18181 ataacattta tcacacaccc tcaacgcctt atattgcaac tgttaaactt caatttatca
    18241 caaatatcgc tcactgcaaa atctctacat ttttattata tcataaaaat attatttttg
    18301 aatatctgta ccaatttgtg gtataataaa agcaaggaaa taatttattc ctacaaagag
    18361 tgattatttt cagttgttaa gtgaaaatat tattttttta aatcaccctt attggcgtct
    18421 gggtgatttt ttattttgtc ataaatataa gctgatataa caccagctag tatgcttaat
    18481 aaaaaaccta tcatataatt tcacctcctt ccttatttgg aatttggcgt ttaatatgaa
    18541 aataatcacc cttcgcactt tcgattatta tccttgctac aattattata acctataatt
    18601 attacatatt tttccatttt ttttatataa acaatgaaat tcaagtaaat aaatacctac
    18661 ttatttatat atattttata aattaattgc tttataatca agttttcatt tttttaataa
    18721 aaatttttat tttttatttt atataaacaa tattttttct aatttgtggt ataataaaag
    18781 caagaagaac tacaatctat ttgacagtag agtgaagttc ataatttaaa caattagtaa
    18841 ttatttaaat ttgtggaact tgattttaaa atcaaattcc cagccacttt tactcttgcc
    18901 acgagttgag tggcttttta cttttagaaa tactttacaa attaagcaaa atattaaact
    18961 agcaataacg ccagctatta cattaagtaa aaagttattc atacttccca cctcctttca
    19021 ttaggaagta ggttttatcc cagtatgaac tccactctat aaattgtaga ttacatcttc
    19081 ttgctaaaaa tattataaca tataattatt acatatttta cctattctat atttattttt
    19141 ttattttgct atcttcttcg tcccctcttt ctctctcttt cagcttcttt cattgcttcc
    19201 tcttcatctt ctatctttat aagtattgag gcggctgcta acgctctctc attaacttct
    19261 aaattcatat attcacttgg cttccacttt aatttttgaa tacaataatg agtgatgcta
    19321 gcatcaaaat cgccacctct gattagtttt ttgcttcttc tactttatcc tcaaaagatg
    19381 tatcaaatcc attgacttca ttcactttta ctgtataatt gacatactca cctgctgtaa
    19441 gcattgtctt taataactga gcttctccca ttactccata actattttgg agttcggcat
    19501 cctttaaatc tggaaatact gtagatgcta cacataattc agctacataa ctattgtagt
    19561 caatttcact tgtatattgt ccagtatgct taccattgtt accaatcact tttactcttt
    19621 tagtacactt tcttcttagt gcttcgtctt cttcagatga taaaactttt aattcccatt
    19681 caactggttt cccttcttta tctaaaaatc tgttactcgc tacatatttt acattatcaa
    19741 cctttattgc attttgactt aaaaaagcac ttaaattact catattattc taatctcctt
    19801 ttattttaat ttttcatata aaaaatacac atttataact tataaatgtg tattttactc
    19861 catacctata ttttttatta cttcttccaa tggaattttt ttagtaacta attgattata
    19921 aagaaataga tacttaacat tcaaattttc tgcacattgt tttaaagtaa tcatttcacc
    19981 tttataatta actctaatac tatttctttt attatttcgt tgaacttccc aagtaaccca
    20041 tctacaattt gatggttcgt aatttccatt cacattaatt ctatctattg ttaaaccttt
    20101 tctatatcca ttttctatag accagttata aaaatttata aaatcctcta gccattcatt
    20161 gcatacttct atgcctctac ctccataata attataatct ttattactct tatttgtgca
    20221 ccttttaatc atacggtgat aaactctata taagctatca ttagatttat tatgcgttgt
    20281 agagcgctct agtaatattt cctttcttat acagccacaa ctttgtgctt ttccttgttc
    20341 taatgcactt gtactagtta ttgttctatt tccacattca cattgacata accataaatt
    20401 gtgactatat ttatctttcc cagacatttt caatactgta agcttcccaa aagttttccc
    20461 agttatatct ttaaatctct tattttgtcc tttccagttt ggagaacaac cacaatgtgg
    20521 aggcttctca tttttaattg attgctttaa attattttct actctctcaa ctttattccc
    20581 acactcacat tgacatagcc agactttttt actatgttta tttctcccta aatatttaat
    20641 taaagtaagt ttgcctaatt taattccact ttcaaattcc atgaaatacc tccaaataat
    20701 ttatacttac atcataatat ttttcaagta taattcaatt ttataacatt ccattaagta
    20761 atgcaaagga ttcaactaat tcccaatcct caaaagtgaa atccatatct tcatctaaat
    20821 actcaccatc agcatcaaat ttagtaatta ttccactgtc catattacaa tctttaagta
    20881 ctactgtctg tcttcctaca gcagatgtag ggtcttcatt tgtaacttgt atgtcaaaat
    20941 aaatatcctc accagtttct ttatatctgt aaagtaattc tctaaaaata gaagtattat
    21001 aatgaaatgt tgcacttcca gtatttgtac tcccagttgt tttatttccc tttgttgttc
    21061 ttcctagaat tggaacttca cttttatttt tttccatttt agcctctaaa tctatagctt
    21121 gcataaaatt atatctttta ccttctatag ttataaaaca ttcagctttc tttgcactaa
    21181 ctgtatcttt agcatttatt gtttgagcca tattatcact ctcctctcta attaactgaa
    21241 acagtcatat aaagcttact catagcattt attaccttaa tagcatcaga tactatgaca
    21301 gttttcttat catttccaag ctctacacta acatcatcag ttttaaaatc ttctattgcc
    21361 cttatattct ctaattcttt atggtgttta acaacatcat tccagaaact tattcttcct
    21421 gccttatcat tcggaacttt acctaaatac ttttcattaa ataaagttgc aatatcatta
    21481 gcaatttggt caagtactct aacactttgg ttacttgaaa aatcgtcatt tttatcatct
    21541 gtaaatgata caaaagtatt tatatcctct aacacatgaa cttcatctcc caccttgtgg
    21601 aatataaatt taccagtttt taaagcttct tcaagttgta tttgtgtata attaacatca
    21661 acatcaaact caccatcata ctttttatta gtattagatt tatttatatc gcatcctgct
    21721 atagctccag tagcccaata aatcaagcta gattcaacta aatctttatc tttaatctta
    21781 ttttctatag acactacacc ttcataatct gcatcacttt tcttatatag tactgtttga
    21841 aacttagctc ctaccttatc tctcattctc tttgtaaatt ctacaaataa acttttaatt
    21901 tctgttgttg tagccaaaca ccctagtgca ttaaatgaat aactttctat tttatccaag
    21961 aaagcttggt actctgctcc tgtcacagct tcgccattgg ttccaccagt aaatacaagt
    22021 cctgcacttg cttctagtgt tgcatccttc ttccaagtga tatagtcatt gtcttgtaag
    22081 tctgtaataa cctttgctat ttgagtatct accttcttat tatctaaaag tattacaaca
    22141 tcaaacttag cattatcatc tatatttgtt gtaactgtta cttttaagtc atttcctctt
    22201 ataccactat attttgctgt ggctatagta caactggctt taacgccttt atttaattta
    22261 taaaaatatc ccaaccttat atttttgaat aaatctctca aacctttcag cttctcatga
    22321 gtataatcat atccaaaata cttcactgaa tacttctcaa aatcatcact ggttacttgg
    22381 aatgcttctt catctatgcc ccaatctaac tctaaaggca ttgcaacaat acctctatct
    22441 gataatgaac tggttgccct cttagctgag ataaaattta tatatgcacc aggaagtatt
    22501 ttattttgcg ttacgaatgt tcctccacct aaagccatct aactcactcc tttcataaat
    22561 ttatttattc tatcctctac ctctgagaag gaatataact cattttcttt taaaattgca
    22621 tttaataaat cttttctatt tacatacttc ttagagttaa ctatctgctc cttagtaaac
    22681 ttgtagttat cttctttgct taatgcttta ctcaaaatta tcacctctct tcaaaccacc
    22741 gaataactct actgtatcca tcttattggt atcattattt tttatagtaa aatagttata
    22801 atcaacaaag aagtgaagaa cattgtctat aatttcaaaa ttcatatttg tacctcttac
    22861 taaatctcca tcaatttcta tatactctaa ttcctccagt agcatctcag ctatctcatt
    22921 tatttcaaaa ttcttatctt ctgaacgagg gaaataatgt acatcaaaag aatttttctt
    22981 taattctctc ccgcttggat atggtgtctt gcttggattt aaaggaacaa taaaaaaaca
    23041 aggttcatta ataccttgct ccacatcttc gctataaatt gtatattttt ctccaaacga
    23101 tttatctaac tttactgata ttccatctat aatattatta agcatcaaac actcctttaa
    23161 gcaatattaa tagttttttc tctattatct tatcaacttg actttgtagt tccatttctg
    23221 aaatagttaa gaaatgttgt cctttaaccc aacccttgcc atttttagtt ctatgaccaa
    23281 attcaacata tgcattttgt tatcctaaag gctttttatc ctctagctct tatagtttcc
    23341 tataagttcg gcgtacatca tcaacaaaat aaactttatt tagttgccca gcactcttgg
    23401 agagattata tttattcact ctctacgctc tacggaaacc tatagcctat ccgcaatcta
    23461 taggttttcc tcggtattgg catatataat taattaaata tttttccatg ttcttctatg
    23521 gactatattt gatatagatg aatatgttac aggatatatt tcacttagtt gttttattgt
    23581 atatccatca gaatactttt ttctaatttc ttttacatcc tcaatagata acttagctct
    23641 gctttcttca ataatcttaa ctttttctat aagcttattt cctactaatc ccttagaata
    23701 tctacttctt aagcaagagt atgaaatacc tgttttttca gaaagctcta tcaatgtaat
    23761 ttctttttct tcatgtttta ctaatatatt tgttgaccta tttctacatt gtgtctttat
    23821 atccacccat ctacaattgc ttgattcata atttccttca ttatttattc tatcaattgt
    23881 aagtttctct gaatatccat tactcaatgc ccaatttgca aaattatcat agcaaaacca
    23941 ttcatcacac acaattatat tcctttcacc atatctattg tatcttttat tatttttatc
    24001 tgtacatcta gatattatac ccttccatat actgtaaagt cttttatttt gaaccttata
    24061 tttcttttta aattggtatt tgtctgttaa atttaattta tcttgttctt tttttaaaca
    24121 tccacaagat tgcactaagc cactagttaa agagtcagtt cttatttctt tgaaatttcc
    24181 acaatcacac ttacataacc aatatttcct atttctttta ccgcttttta tctctttaga
    24241 aaatttaatg actcttagcc ttccaaattt ttttcctgta atatctaaaa attttgccat
    24301 aaaatcacct cttttataca ttataaatca agagttattc tatgtccata ttctacctac
    24361 tttataatgt aacactatta cacttagcgt ttaccgattt tgctgggttt tatatgcccc
    24421 attgtgttaa ggcatattca gttggattaa caacttctat aatataatta ttaccttgtt
    24481 tgtaaacagg aagcgaccta gcataagcta ctccactcca accctctctc aaaaatcctg
    24541 tatcaactgg tgtccttcta attacttttc ctaagagtct tgctgctaat tctcttgctg
    24601 catccttgca aaacttatct aaatcaatct ttgtaaattt ctccatcttt ttacaaactc
    24661 ttttaaactc tctaaaatca acactgcccc atctagccat tatgctttat ccttaaataa
    24721 ctcaagtatt atttcttgat gatttggata tatagctgat tctccacttc ttacatactc
    24781 tttatcattt ataataagtt ttgaacctgc tttaatttct atatctggag atataaagag
    24841 tttaatagtt tgctctagct tagctaattt cccttctgta gcagaaacta tatttttata
    24901 tgaaagttta catggttgat tttctaatac aatcacttct ttattgttag ttcgttttgt
    24961 tacagggtct ttaattggct gatactcaac tatagtacat ttatctctat ataacatttc
    25021 tattgctttt ctagttttac ttaccatctt aagcacctaa aggttaatat cttattctta
    25081 ccataagtag taagataagc tattaagcta tcaaagcgtt gttctggtgt ttgagaacca
    25141 cttcctatag caaaatctac ctttgtatca ccttctgata tagacttttc tacagcttca
    25201 aagttaatgc tttctatatc taattgaccc atatttttct tggtaaataa gaactctcca
    25261 actatcatat caacttcaat ttctttcaat tcaattggca tagtttttat attacaatct
    25321 agtttaataa tattttctat tttttctctt acaaaaccta ttaaccactt atctccatct
    25381 tttaatatat atccaaaact ttcaagtctt ttttctattt catcaattat attattttcc
    25441 ataattttca cctacttttt agtaagttta tttttctctt taagctgctt attttcttct
    25501 tctaaagact caacttttga ccttaaaata ttattttcag ctattaaatc ttttacactt
    25561 aatgacttgc catactttac tgccttacca gtttcatcta tcaaatcata tcccatctct
    25621 aagaaatcat ctattttaca ttcttctatg gttagtattc tatttagttt ttttacttgt
    25681 ggcactatat atcactcctt tttctatgct tcaacaacaa attgtattgc atcagctttt
    25741 ttatttaata taaatacatc ctcaaaactt tcttcaaagt agaagtattt tccctctgta
    25801 actgctgttg gttcgtctaa cttagagaac tgataagaaa caggtgtaat tattgcactt
    25861 gggtgaacta aggacataaa gatttgttta gctcctgctc ctactttcca tccagttgta
    25921 aaatcatatg cagttttcat tagattagat ggtactttaa ttattttaac tgtgtcaata
    25981 tctgtggttt gtctatttaa agaagttcca ccatctttta tgtttactgt tctttgtatc
    26041 tcttttgcat ttttgataag tgtatttact actggagtaa catacaatat tcttccattt
    26101 tcaggtactc tagcttctgt cattttttcc attaacttat caaatacttc taatacgttt
    26161 gctgttgtaa gaacagttgt atctgctgtg ttacctaatg cggtccaatc agcatatatt
    26221 ttagatatac agtaagcatc catctctgga aacttttgtt cctcattata tacttttgtt
    26281 atattaccta ttgaagccac ataattagtt tggtttatat ctgctggatg aaccaatgtt
    26341 gaccatttcc tttgattagt taatacctta ggttcccaag cattatcata gtttctttga
    26401 gctactgcta ttgtatctct gtttgaatct acccttccag ttgtagatat agttggtatt
    26461 tctattgttt tagaacccgt ccatctatat cttccattat tttctgttgc atacaaatcc
    26521 ccgaagttta aagtataagg atatgcttgt gctaaaacat ttgaatattc ttttgcataa
    26581 tttagtgctg ccattttatt tcctcctatt tattattatt ttcatgaggt cttaccccag
    26641 taaaattaaa accaaaatca tttatcttag gctcttgccc tggtgttata gtatctattt
    26701 taggttcttc accttctagt gttgcattaa acaaataatc tttatcctgt ttcaaagggt
    26761 ttatttgctc ttcaaaagct ttttgtctat ctttactatt tcttagggct tccatgtcta
    26821 aatgagcttt taacgctatt tcatctctac atttaacaga tttaaaagca tcacctaacc
    26881 agtaattaaa atctttttct tcaatttctt ttttgtaggt ttcttccaaa gttttcttat
    26941 cagtttcata agttgttttt agattctcta catcttcttt tgtcatacct ccttcaaact
    27001 ttttaatagt ttcattagct gtattaagtt gtgtttcaag atttacataa tcttcttgag
    27061 taactgtagt ctcctttatt ttcttttcta tagatttttg aagagaagct acatcaattt
    27121 tgttatcctc tacttttatt ccttctagca attcttttaa ccaatccatt ttaaatttct
    27181 cctttcattt tttacaaaat aaaagcacct actaatttat tatttagcaa gtgcttttac
    27241 atatttacta tttgtatatc tttccataaa tcctttagta atttaccatc aatgttgtaa
    27301 ttatcaacca tatccttacc atttttataa tactttgtat ctccattagg gcaaagagta
    27361 ataaaccttg tatcatcatc tccaatagat atattatatg gtttattata taagtcaaat
    27421 tctatatcta atcctaaatc aatagaatca attaaatgtt gtaaattctt aaatttatta
    27481 tccattttat tctccattca aaatatcttt gttagctatt ttatgagctt ttgtaagctc
    27541 catatccttc tctcttttta ccttatcatg gttattttca tcagctaacc aatcatgttt
    27601 atgaggtaca attttatgtt gctttgggtt tctatggtca gttaagtcta aatctaatct
    27661 aggttttcct gtattaccat agtatcttct ttgaattaat ttaccatctt tgtaattatc
    27721 aaatacacta tttggttttg attcaaatgg cactgaatga acacttccac tagttaaatt
    27781 tctctgattc ttaacttgcc aatttacatc cttataaagc tttttagctt cctcatacct
    27841 tatagtatca ttatacttca tatgttgata ttcatcaaat ttagaaggta tttcatttcc
    27901 taataccttt ttatattctt caaattgttt tctatcttta ctctcattta gttgcatttt
    27961 tctaagggtg tcagcttttt cttttccaag tctaccctct atatgtttct cataccactc
    28021 attatacttc atactagatg gtacataata tgtttttcca tcttctcctc ttgctgctct
    28081 atagccttct tcatcctcga accaaggagc tgttgttgtc ctacaacgac aatgaaatgg
    28141 tggagctgtt attccaactt gataatcttt catatcaaat atttttccat ctaactctct
    28201 gcatatattt gaagttctta aatctaatgt agcaataatc tcatatttct ctacatctaa
    28261 atcactaaaa caatcttttc tacttgctga tgcaaagaaa gctgattcag tcattatcaa
    28321 attcttagct tgtgatttag atacattaaa tctcttagca aagtcattta ctagattttt
    28381 tggattttca cctctaataa ttgattgagt tagcttagta tgtagttcat ttattaaagt
    28441 aggtctatgc ttaccccaaa ttctttcact aaagtttaat ccatctgttg cccatggttt
    28501 agagataatt ttatttattc tattagtatc aagactcatt aaactccaac caacgtttac
    28561 tccttgttga acattaaaag ctgtatgata gtatccactt gtataaatat ctctcattag
    28621 tttatcaata ccatcaagtt catttccata taaaacttct acgtgttgct gtatttgtaa
    28681 ctttaaagct tcaagccttg ttatatgaag tcttgcacta gcattttcta actccttcat
    28741 ccacttttga tttatagcat tttctttgcc atatttaata tactcttcta cattccactt
    28801 aaattcttct aattctcttg tatttaacag tttcttagct tctaataaag atattccttc
    28861 attttcagca aatctgttat accatgataa tatatctttt tctatactat tcatagctaa
    28921 tttgtatttc ttttccaatt caagataata ttttacactt ttattgtttt gaacttcttc
    28981 taattgttca aatcttttcc tccaataatc tttatgtttc atctataaca ccctcttgat
    29041 tattagtagg aattaaatca tcatattctt tttgggtttc ttcctgtttt ttaagtcttt
    29101 caagttcgtc attaacatct tcaacccaag gatgattact aacaatagtt tcatctgata
    29161 taattccaat tgatttagaa gccatatcta ttttttcaga ctcatttatt atcatagaat
    29221 gattaaatgt tatttgaact gacttataat catagctctt actaccactt atctttaaat
    29281 actcacacac aaaccataaa agctctctaa ttgctttttt aaacttcttt tcagtcttgg
    29341 aacattttaa atcaagtagt gaatataaaa atttaagagc tacacccgat ttgtcacctg
    29401 tgttttgaga ttcgggatta actccttgac caaaaataat tatattcttt tctaatctat
    29461 caagaagctc ctttttggct tcaactggta tatttatctc tagtttatca actccacctc
    29521 caccatctac tttaattgat ttatagtatc ttatattatc tataaactct tgtagacttg
    29581 ttcctggata ctcttttaat acataaatga cctcttgcat ttcatctaaa ttgtctgcaa
    29641 gtgtagaaat gttattatca tatatatcaa ttaaagactt atagaaagtt aaatctgaga
    29701 cacacttttc attattttta aaaggtataa atggaacttt accccatccc tgttctttgt
    29761 tatttattct aaaatgacct tcttgtatat cagtcatttt tccatattca tcatataaaa
    29821 attcttgaat aaaactatta cctctttcaa taaagtaagt tacgtcattt tctgtgtagt
    29881 actcaactct ttttatttta tttccatcta tatcttcaat ataataaaac ctaataaatg
    29941 caactaattc cctctgtctt ttactatccc aaataggaat tgcttcttca gctggaatta
    30001 ttacatattt aaactcacct tttctattaa tatatggatg taaccattca acccctttat
    30061 tactagcatt gagatatagt tctgttattg tatcgtcaaa ctcttctcct agtaagtcat
    30121 ttaaaagctt agtgagatta tcatcatctg cattaaatac tatgggattt ccgacactat
    30181 agcctacctt ttggtcaact aaaagcttat ggtagttgtt aattgcttta ttattaactt
    30241 tagtaaaatc atcaacctta gctccatcta agagataata tcttctctta ttgtttacat
    30301 cagtattacc ataatagtat tcttctcctt gtttatattt ttctggccta tgctttaaga
    30361 tgtagtgttc tatgactttt actaggttaa aggtgctctc tttttttaac tgaactttta
    30421 ttaaatctgt ttcacttata taaatattta acacctcctt tactttaaga agcttattcc
    30481 attattttta agcttattat ctatagaata tctaagagca gccattgcat catccataaa
    30541 ctcaactggt tcatcaagat ataatccagt tctttcgtct tgtttccatt tccattgttg
    30601 tatttctttt atggtattag tgcaactagg atgtacatgt attcttaatt gtttcaaata
    30661 atctatttga gctttaacac ttcctggtcc ttttttaact cctttagctt tatatcctgc
    30721 actcttccac atcttaattc tatctggttc agcactatca cagtacataa atagagtctt
    30781 ttctaaacct ctactatttg caatttttat gatttctgag gtatccattt catgtacata
    30841 tatttcatta catatatata actctccatc cttaaagcca attctaagta ttgcatttgc
    30901 atgattaaag ccaaagtctt gtgacagtct catattgtca aagtattcaa attctgtggg
    30961 aaattcatgt ataacataat ttttaagtat tgctccacca gtttctcccc attctccaag
    31021 accatatact ttgtaccctt ctgggtcttg ctcttttctc atttgcattc ttctgtagta
    31081 agcttcatct atgaatctat tttgtagata agtactatga tgagtaaata tatcatcatt
    31141 tttatagtca aaatactttc tttttatcca atgagtagct gagactggat taaatgtaaa
    31201 tgtcatttga taatacaagt taggattagt taaaatacct cttaaacggt catctagtat
    31261 atctatgtca ctttccataa gttctgtagc ttcttcacac caaacccatg ttaattttcc
    31321 tttcgagaag ttaattgatt ttaatttttc tctttgtttt gcatcattaa ctcctctgaa
    31381 aattatagag ttaccagtaa ctttactctt aatttctaaa ggatttaaag tagttttcca
    31441 atacttatca gcttgtttac cataaatacg atttatagct cctgtaagct ctgcatacgt
    31501 tgaatactta tgtgtagctt ctgactttct aactactaat agattagctc cttgatactt
    31561 cttatctcct aactttagta tatagtcttg tgctacatta acagattttc cactccctgc
    31621 tgaacctttc attgctctgt atcttttttt agtaaaatta gcttccttga aatctggatt
    31681 aaaatttatt ctaactatca tttctatcac cataatctac acttattttc aactcatcat
    31741 ctccaatatc atctttattt aggttagcaa cctcacattt caacttctca actcttgttt
    31801 tctgctcctc tgtagccaaa ttccaatcct tatgaatcat ttcatcatac tgcttaatta
    31861 aactccttaa ctcactcata gctctactct gtgcattaag aaaagatgct tgcctatccc
    31921 atgcaaattg aaattcatac tctatcttct cactattttc tgtgatttca tgtttcttta
    31981 attccttaat catttcctct ttatctttaa catacattat cttctgtgct cttattattg
    32041 ctgcgtattg aattgttatc tgttcccaaa gaatatcaaa tttatctttt atagatattt
    32101 cttgtattaa ttccctagtt tcttcaggta agtattttga gaagaaacca aacttttcag
    32161 catttttatt ctcttttgga gcaccataac caactgaatt tttattagaa aagggtgcac
    32221 cccttttatt tataggtgca cccttctttt tttcactaga ccaattgtat ctttttatcc
    32281 atgactttaa agtatttaaa ctaatgtcat actttgctga tatttccttt tgtttcattc
    32341 cttttaagta atcttgtttt accttttctt tgacatcttg cacatcacca cctctttatt
    32401 tgtttgtttt gggaataaaa aaacaatcct atttaatagg attgtttcaa atctagtctc
    32461 ctctatcctt ccaggataaa tgaaacatac ttattattaa tgtagttaga gataaaatta
    32521 tatgaactgt aaaaaatatt aaaacataga aaattatatt agaataatat cgagtaagca
    32581 ttattgtatt gataaaacat attacaacaa ccaaaatctc cattattaca gtataatatg
    32641 cttttaactg tatgaattga aagtatgata tattaaatcc atttaccttt ctttctgttt
    32701 ttatatcttt tgcttctgat attgatttgt tatatgtagt aaacaataat gtaataatgc
    32761 aaactccaaa agctactaat agtcctgaca aagttagtac atctttgttt aaagaaatta
    32821 caacttcttt attaattgac gaattaaaaa acatgtatga aataatacat attaatactg
    32881 gtgttaaaac taactctact acttctttag aatttatagc cttgtaataa ttgagtaaga
    32941 tatttaatac ctttttatac attttaacac ctccttagca atctatgctt cactcatagc
    33001 aacttcttga tacattaaat taaaaaaaac tgaattattc tcaattaaat catctatcaa
    33061 attattcatc ttctcaaaaa tgtcttcact atctatatga ttatctatat caagagaaac
    33121 ctctatttct ctacacaact ttataccctc agtatccaac tttaattttc tcttattttt
    33181 ctctccctca attattatcc tatttatttt tttatttcta aattcctctc ctttaatata
    33241 tttttcgcaa catcttttaa cttcactagg taaatatcta gttctaaatg tgggcttata
    33301 tatcaaatcg ttatgtcgtc ttgaattatt taaattagaa aacaaattat cttcatcagt
    33361 tccaaacttc tctctatcaa catttaaagt tatttttgaa acaccatcta aatctaatat
    33421 ttgttctata aaatctttac ttgctatttg tgtaatatta attgatgttt ttgacatatt
    33481 attatcaaat ttattatttt caatcatatc ctcataatat agatgtaagt ttttacttag
    33541 ttcaatcatg ggaaccccca caaaaactct ttcaaataat actactgcta tattattttc
    33601 cttcataaat ttaataaata ggtgctgctt attgctatct ccttgatcca tttttttatt
    33661 accctctact tctttagtat gagcatttat aatatttgta ttctgattgt atttagtata
    33721 ttcaagtttc accttgatta ttaaatcatc ctcgctacat tttacttctg taatccaaaa
    33781 ctttttatct ttagtaatat taattccatt tttgttttct ttacaatatt ctttatcctt
    33841 aaagaaggtc tttattacct cttttaaaat ttcttcatct gaatagacac tcccattaat
    33901 tttatttata atatctattc tataaacatc aatatatctt tttttaaaat tactcattat
    33961 accattattc ctttcacaaa tatttataat gatataattc tatataatca gtcaatatcc
    34021 ttcaataatc attcgacaac cacaaaataa gtctaaataa tacttttacc ttattcctgt
    34081 ctataatgta caaatatatt tcatttttaa ttcattacat aaaaaaaaag accctctatt
    34141 aatacagtca tttaaatcat ttcgattaat tctttaatct ttttatatac ctctttataa
    34201 ttcatacctt tatctatcaa cctaggtaat ttcatagata taactctttc aagtacttgt
    34261 attaataaaa aaactcttgt atttcatctg taaaatcttt tctgaccttt taccttctgt
    34321 tcttatatcc agccattctt gatctttttc tgtggcaata tagtaaccat gtattctaat
    34381 ttgtttaagt gcttttgtaa cccacttcgt aaatagtttt gcttctgctt tattacttct
    34441 aaatgacata ttgtacacag cttcttctgt aacaaaagta gtaccaaagt taggcaattt
    34501 atctttaaag tttctagtgt aggaatctcc gacagtagac tcattaaatt tctttttata
    34561 ttctctatct atatttctta atgtatcacg aatatttact atgcctagtt cctctcctac
    34621 gtcatttgca ttaaaccaaa cttcttctcc attttttgac catatcatcc ttacattttt
    34681 ctcttgtaaa attttcaaca tactactacc tcctaaatta gttattaata aagaatattc
    34741 aatttgaaca cccttatttt cgacccttca atatgagaga ttaaaattgt ataaaaaaag
    34801 acctagaaat cactctaagt catgaattac tcatttatca ttctccttat ttacaaaata
    34861 aaaaagacta agttggggta acttagcctt tttcaagggg gaatatatta tacacttgtt
    34921 tcatactacc attataacag gggttaaata tcattaaaat atcatctttt tatcatgtat
    34981 ttatcaaaca gttaatttca agccatctat accaaataaa tacactccaa gttcttttac
    35041 catttcatta atccaacgtc ttgatgtaac tactccacaa tttaatatct ctgctatttc
    35101 ttcatatgtt ttttcatcac aatagtaaag ctctaaagct ctatactttt cataagaatg
    35161 taaacgctct tgtcttaatt tcaaagtttt cattgctgaa tctatatgtg ctatcataat
    35221 aatagttctt gctttacttt tctttatact taaaatatac aactcttcat tatcaagctt
    35281 acctaaatca tcctctaagt aatctacatc tttagcttca cttaccgctg agtctatatg
    35341 tctcttaaaa tcattataat gcttcatcaa taatctagta ttatataaaa cgcctttttt
    35401 cctatcttct ctttcttctt ttcttaactc tcttacaatt tcttttatac tttctttatc
    35461 cacttaaatc actccttctt gctctcttcg aacaacttct actgcaatac tcaactttag
    35521 atttatccaa cttataaaat cttctaccac accaagcaca tcttactatt ttacctgggc
    35581 ttataagttc catcttttct ttttactttc ataccttcac tccctatatt cttttaattt
    35641 tgcttttact gcttcaagta atgcactttg ccctttatcc ttattctcta tagcttccat
    35701 tacttgttca tctattgtac ctttacagat tagatgatgg ataataacag tttccctttg
    35761 accctgtcta taaagccttg cattagcttg ttgatacagt tctaagctcc aagttaatcc
    35821 aaaccaaaca attatacttc cacctgattg taaattaagt ccatgtcctg tacttgctgg
    35881 atgacaaagt aataattgta ttcttccatt attccaatca tatatatctt ttgagttttc
    35941 tattgttctt ggtttcaaac tcttaaactc tttcattaaa cgattataat catgtttata
    36001 gttataaaaa actataatag gcttaccatt tgaaacatct ataatttctt ttaaagcttc
    36061 taatttttct ctatggagtt cctttacatt tttatcatta tcataaattg ctccattagc
    36121 taattgtaaa agtttatttg ctgctacagc tgctgatgaa gctgttatta tatctctatc
    36181 taattctaat atcttctctt tttctaattc tttgtaatat ttaagtattt tactatcaag
    36241 gtgtatttca attttattat ctatttttct aggcatattc aaataatctt ctgcttttaa
    36301 gcttatacaa atatctttta ttttattatg tattgcattt tctgctccat ttttaggttg
    36361 ccaattatat atagctcctg tttgataatt tttttgtcct ggctcaaagt atcgttcttt
    36421 atatcctgta atagttttac ctagtctttc acctctatca agtaagtaca tctgtgccca
    36481 taagtctatt aaactatttg gtgttggtgt accagtaagc ccaactactc ttttagttaa
    36541 aggcaatact ttctttaaac ttttaaatcg ttgtgcttta tttgatttaa aagaactaag
    36601 ttcatctatc acaaccatat caaatggcca ttttctttta taaaaatcca ctatccaggg
    36661 taccatttct ctatttatta tataaatatc tgagtctgta cttaaagctc tcactctatc
    36721 taatttacta ccaagaactt tagatacttt tagatgcttt agatgttccc atttttttac
    36781 ttcactactc caggtatctc ttgcaactct taaaggtgct ataactaaaa cttttgatat
    36841 atcaaagtaa tcatacatta attcacttat agctgttaga gtacagactg ttttacctaa
    36901 tcccatatct aatagtaaac ctatattatt attatctata gtttttctaa ttgtgtattc
    36961 ttggtatgga tgtggtttaa attccatccc ttaacctcct tgataaaatc atctatctct
    37021 tttaatgtgc ttacacactt aactttaaat cctaattctc ttaattctcg cattttatat
    37081 tgttgaagct tccttagttt tttacctggt gctttaagtt ctacaaatat aacatgtcct
    37141 tctggtaata agacaatcct atctggcaca cctgcctcac ctggtgaaat aaacttcata
    37201 gcctttccac ctaacaactc aatctctttt ttaagtcttt tttctatttt tgattctaac
    37261 aaaatatcac ttccttaaaa tttaagtgtc aacaatgtca acagattttc tatatatatg
    37321 tatatataca tattaggcat acatatgtat atacgtatat gcctaatatt acttatacta
    37381 ctttatactt aatttttgtt gacattgttg acattaatac ctcaaacatt gtaattactg
    37441 catcttaggt gtcaacataa gcgtcaacaa agccattttt ttgttgacat tgttgacact
    37501 tttaaaattt tcaaatttac tctgttttta attctgttga cactaacttt tacgtacata
    37561 agctctctgc ctaccatata ttttcccaaa ccttaaattt ccattatatg atatccaatc
    37621 atctaatcct tttaatatat cattaatttc tcttgataag ataggcgtga gttgtttagg
    37681 ttctccatta aatagctcaa cccatatctc cataacacac gttttttctc ttaatattgt
    37741 tccttctttt aaatcaccaa aatctgaacc atgaatatat tctctctttt ctgaaatact
    37801 taaatcatac caattctcag taataggttt atttagatac tcttcgataa ttccagattt
    37861 agcattttct tctgagtgag ttctttgttg tctttcagct tcttttttct cctcatctga
    37921 tagatatagc tgttcattgg ctttacataa ttctactgct tctgcccaaa tttgatttct
    37981 ttcattatca agttgaccat taaatatact cttgttaacc ttctttactc ctgtatctat
    38041 tggccaaaac cttctattcc cggtcttatc tcttaaaaat tctttatcat tagttgttcc
    38101 tataactaca cattgacgta aaaaccttga tgttctcttc ccatatgcga ccctatatat
    38161 atcctctgat ttgcttagaa aatgcttaac tgcctcaata tctgcttttt tagtagccat
    38221 catttcaccc atttccagca accatactcc ttgtaattgt tcatacgctt ctttaccttg
    38281 tacagtagtt aaactatcag aataccagtc tccaccaagt tttttaataa aagtactttt
    38341 tcccattcct tgaggaccag ataaaaccat catattatca aactttattc ctggattaaa
    38401 tactcttgct actgctgcta ctaaaacttt tcttattata gtccttgtat agtggttatc
    38461 ttctgcacct aaatagtcaa ttaaaagtgt atccactctc ttaatgccat cccactttaa
    38521 agaatttaaa taatctttta taggatgaaa agtattattt tcaaaagcaa ttattagagc
    38581 atcatttact tttgatggtg atgagatatt gtaaattgtt tctatatgat gtctaagccc
    38641 agaatcatca ctatcattcc aatcatttaa tttatcatct tttctccaag gtaacttacc
    38701 taaaacgaca gctctatttg aaaattcatt ataagctatt tttcctttca aatatgggtc
    38761 attttctata aacattaaga tattgtttgt tgttttctta taacttccct tattgtcata
    38821 atctaaccta gttaaccatt catcatcttc aaaatctata tcaccaaaat catccttagc
    38881 tttatcaagg ttttctcttc ctatagtctt tcgtacttta gtgtcactgc ttgcaaattc
    38941 gctcattcga gtaaatgaag gtaatctatt tacaggcgtt tcgggtttag catcttcatc
    39001 aagttcacca aatttatgta tcctaactaa atcaaaagca ttgcataaaa ttccgcttgc
    39061 tgggtctgta ccatgatgac tatatgaaaa cttgtcatca taaataacta ctccaccact
    39121 tgtactacct tctgaatatg tatacctggt ttcatcaata ccaggaatat atacttcatt
    39181 taagaaattt tctatagttt cttttatact ataggacctg caaaatgcac ctataattcc
    39241 agccttttca atagggtctt gttgtttttt taattgtgta ttaaactttt gcctctccct
    39301 cgaactttct ggccaatagc ttacatctgt ccagtctaaa tataaatcta gtatttcatt
    39361 tgggtttaaa aactctccat cttgaaattt aaatatatag tctccatcaa ttgaagtact
    39421 tggaaaatac ataagtctat gaggttgata tgtagtatca tcaaacatat ctattcctat
    39481 agtatctgct atcatcctgg aaattgcttg atattcttct ggtagtactg gtctagttaa
    39541 aggtattaca agtctatatc tgggattatt ctcagtatgt gagtgcgtag aatacataag
    39601 acaagcataa tcatttaata atgttatatc ctcccatata tctttattcg cataatctat
    39661 atctaaagtt ataatacttc tatttgcaat attctctgct tttcgtcttc cattttttaa
    39721 gcttccacct acaaatccac caacatcttt aactctatct ttttcagtct ttgacatctt
    39781 cctatattct gtatatgttt cttgagttct taatgtttta cttaatctat ttacaagttc
    39841 ggaccataaa atacttttat ttttccaatg tgtttctaat ttattttttc ctatggccag
    39901 catgagttgg ccatcatgtc ttacatttat gtgttcaatt tcactggcct ttatatccat
    39961 aaatcaatca cctagtcttt cttataataa tcacattcat atccatctgc tttaagagga
    40021 agacctttag cccaagatat ttcttttccc ataatactgt taacttcttc taaagaacct
    40081 ccttttttat ctacatcaat tacaagctca tcatgtacat gcattacaat gctataacct
    40141 gcatctgtca cattaaacat agcctctctt aagcaatctc tagctgtagc ttgaacaata
    40201 ttctcaacta acttaggtcc ataagtatct attcttttcc attgtttact tgtctgttcc
    40261 ataccttcat atgttatctt atccccacta aatgtagtat gaggctctat cttaggtctt
    40321 agatatgata atcttctacc acttggtagt tctataaata aaactcctgg attataaata
    40381 aatttaatcc catgttggag ttttactata gtcctatctt ttatggcttt tttagctgcc
    40441 ttatctacat cccaccaaaa tttagttata tttggattag catttctcca tgttgttaca
    40501 agaggttgaa gctcctcttc atcaagaccc atttttatag cccccattga tgttaaagct
    40561 cctatgcttc ccccatatcc aagagctagc tctgaaatct tacctttttg tctaagtggg
    40621 tcaccttttt taatactttc tattggaact ttaaacatat gactagcact agcttcatat
    40681 attttcccgt gagaattaaa tacatctaat ctccacttct caccagcgaa ccaagctata
    40741 accctagctt ctattgcact aaaatctgat actataaatc tatgaccttc acttggtata
    40801 aaagctgtcc ttatcaattg acttaagaca tctggtacac tatcatataa aagctctatt
    40861 aaatcaaaat ctccttcttt taataggttt ctagctaggt ctaaatcctc tatatgattt
    40921 tgtggtaaat tctgtacttg tactaatctt cctgcccatc taccagtcct attagcccca
    40981 taaaactgta gtagacctct tactctattg tcattacctt tagctaattt catagcctca
    41041 tatttcttta tagaagtttt ggacattaat tttctaagtt ctaaaattct aactacattt
    41101 tcatcatcaa cttgttttaa tatttctgga atactttctt ttgttaggct tgtaatctca
    41161 aagccaactt tatcacttaa ccattttttt aattgagctg gactatttgg attatttaga
    41221 ccagttattt taattgcttc ttttgtaagt ttttcagtat atcttttatc acattctatt
    41281 gcattctcta ttaactctgt atcaacttta ataccagtat cattaattct ctggtctaaa
    41341 taccataatt taatttctct ttcggtagtc ttatacttac taagtttgtt tcttatttct
    41401 ctttcaacta caacatcttg tttacaatat tctttaaatt tattccattt ttccatatca
    41461 tgtattggta aatttctagt tcttcccttg ttaacttttg tagccttaca aggtttacag
    41521 aaatattgta ttaatgcttt accttctttc attttctgtt tatcttcatt aaactttaaa
    41581 gccttagaca cactatctaa acttcctgga agtcctagtg ttaacgcctt tatcattgta
    41641 catgaccact catttggttt taaattaata tttaaaaatt tacttattgc tgttctttca
    41701 aaattagcat taaatgctga ttttataact ttattatcat ttaaagcttc tattacttct
    41761 tttggtaact cttcatcatt tactaaatca ataactttta cctcttcatt atcaaaagca
    41821 taggcaaata acagtatctc aaaattagca gagtctacat atctgtagac tccaactttt
    41881 tttatatcta aatcactata tgtttctata tcaattgata aggtcctcat taacttaaga
    41941 agtcctcttc ttcatcttca tattcaaagt catctgagaa atctgcttct gcactagctc
    42001 tagctcctcc aagtacttct ccatctgcta acttttgaac attttgtaag ccacaaccta
    42061 ttcctttatt tcctgcacta ttatatggga aaaagtttat actaactctt ccataacaac
    42121 cactatatac ctctgtatta tctaatattt catttaagtc tttatcaact attcctggct
    42181 tttgtgtact atttgcattt aaaaaataca ttcctacata ttcctctgct tcatcagctc
    42241 tttctgcatc tccatcacga agaggtgttt ttaaattacc tggtaatttt ccaccccatt
    42301 tagaagtttt accttgttct ttagctgcat caatagcctt ctttattctt cctaaagtca
    42361 ccttatctga ttttggtatt aaaatacaaa ctgaatactt gggctctgca ccttctacca
    42421 ttgctctgct tttaaagata ttgcaataac ttaatcttac ctttcctgtt actacctttg
    42481 ttgattgtac tgaattactc ataattttct tcctcctaat atttttatat ttggtatatt
    42541 gattttatat ttgaagttat ttctccaaat ttatctaaat ctctaacttg cttactaaac
    42601 aatttaattc ttccacaaat aactgttgaa gaaacattta ataactctcc tatatcagat
    42661 atctttttat cttgtaaaat ccaatctact attttatcaa tatcttttaa atatgacttt
    42721 cttgcaattt ttcttatata atcaacatca tagttaaatt gaatttcata aaatccatgt
    42781 atatctttta atggtctttt actatcttca aatacactta catctaaacc ttctatagag
    42841 tttctataat gtttttcgcc ataacgcaaa gctttaaaca tttctattct tatgtaagaa
    42901 acagccactg tagaaaactt gcctctgtta ctatcataat ttaatgctgc tttatataac
    42961 ccaatacatc cttcttgata aaactcttca tataagtatg gatgttgctc tacataagac
    43021 ttaaaaaatc tatttatact aaaatgtaca agattcaaat tattttcaac tagtttagtt
    43081 atttcttgat tactcataaa attatatccc cttttaaatc tttttatagc tactaggaat
    43141 taaatatttt taattatcct ttgctcttac tgatacaaag catactggac taacttttat
    43201 atgttctggt atatacactc tatcaagatg attgtcattt acttcgagga aagttgacat
    43261 cactttatcc tttattaaac tttctctttt aaaatccatc actataactt ttgcattttc
    43321 ttcagttgag ttatattctg taatatttag tctctttaac atatctagta aattatcttt
    43381 ttcataatct aaatcttttt cgatactctt ttttctatcc tttaaatcta aaattatact
    43441 tatgctttca tctaatttat cttttatacc tgcatctaat ggcatgtaat atcccccatt
    43501 tatatttcaa aatctttttt cgctgaatct atactattaa tttctgacct tttatcactt
    43561 tctactacta aagtagcttt tccaactggt tttataatta aatcacttag tagtttagca
    43621 aaactctttt tacctatagc tttctccata tcactaattc cttttaaggt ccttggctta
    43681 taaatttttt cttcatcata atctgaattt aataaaacct tagcaacttc ttgctcatct
    43741 acatactttc tattacttct gccttctaca agcttatatc ctggatattt aacaccatgc
    43801 ttttcagctt gttctagtgc ataactttga acatccttta accaatcttg tatattctta
    43861 gcaaaaccta aaatatctgc tatctcatat ttattaagag caaatgtgtc agcaaaatca
    43921 tattttctag ctagtttaag gttatcctca gctctttttc tgcaatcatt tttagctcta
    43981 caaaatccac aatgactgcc acttacaaac tctccttcac cattaaaagc catttgagcc
    44041 ttcttcttaa cattatctgc ccatgtaagt aatttagtaa cttctatttc ttcacttgat
    44101 atgttatcaa gtctaggttg aattattgtt gttttaatta aatcaatatc gtatagcatt
    44161 tcaaactgat tatatgcccc taaaccatat agtctaagtt gaggattctc tatagctgaa
    44221 acttctaaac cttttccata ctttaggtct ataacttgaa gtataccatc agatattaca
    44281 acaacgtctc ctgttccaaa tccttctgga acccactcac taaaatcgag tctttcttct
    44341 aacatcacta ttacatcatc acatatagct ttactatcat ttactaattc aactacattc
    44401 tcaacataag attgtatata gtcctccatt tcagagttat aatactcact tttctgtatc
    44461 tttttaattc ttgcattata tgccttttta cttatttttt catactctag cattaattta
    44521 acttctgcta attcatgtgc aactgttcct tcttctgcat atatactagt taatggtgga
    44581 tagttttctt ctaattttat actaggagtg cagtgaagcc atctatgggc tccactcgca
    44641 ctaagtcttg catgttgtaa tggcatttat gtacccccta taaactttct aatttgttca
    44701 taaaagctga ataatcttct tcttttactt cacttaactt actagctcca aattcaccaa
    44761 ataactcctt aagcttatcc ttcttacctg ccttacttac ttgtgctgcc ttagttctta
    44821 cctcttcttt tgtgtattta acttcactag ttgtattttc attattcttt tctatttctt
    44881 cctttgcttc tgctatttct tctttaacgt cttcttttac ttctaccttt ttaatctttt
    44941 cagctttttt aacttcaact ttcttctctt ctttcatatc tgttatattt ataggttcta
    45001 tctgcattgc ttttcctaga ttaagacctc ctaaagcatt cgccactact aatagtgcat
    45061 ttgtaaattc tggtgcttct actttaactt ttacatttac attaacttca accattttaa
    45121 atatctcctt tttgtgttat actttacttg tgttatattt tattttttat ttttttgtgt
    45181 gttggttatt ttaccaacac ttttttattt aatattccaa ccgatatttt cttaccagtt
    45241 ttaatatctt taaatactat atctgctata acttttccat cttttttaag agttactaca
    45301 ttcttattat tagtatcaag actcagcaat ttcatccccc cactctactg cttttaaaag
    45361 ttcatccaaa tttttacctt gatttctttc aataaaatca tcaacttcat atcttgaaat
    45421 ttttctacca tcacctctaa ctagtgattt tatcaaacct gtgcttacta acctacgcat
    45481 aaaagctgta tctaacttta aaattcccct tgcttcttct actgttatta gataatttgg
    45541 ataacctctt tttattaaaa caactatatc tttaggctcc agtaccttta ccttttgttc
    45601 aatagtttga ctcttaactc tatctgtctc ttgtttactt atctctattt ctattaaact
    45661 ttttaaacta tcactaaatt gttttgctat actctcagaa atatccatgt gaaaattctc
    45721 tcccctcttt ttaattattt atcttccaac taattcgtct aatgtaatat ctaaataatc
    45781 agctaatttt attagagtat ctatagttgg attagaactt ttatttctca gtaatttata
    45841 taaaccactt acatctatat tcaattcttt tgccaattta taaggtttta tatctctttt
    45901 tcttagcatt tgctttatat tgtcgcctat taacattgta ttcacctcag ttctacatgg
    45961 tataatataa acatggaata aattccaaaa ttttcaaaag gatggtgtat tatgcagttt
    46021 tcaaacaata tatctaaaga attacaagaa agtataagaa agacagtctc tgatacttct
    46081 aacacagagt atttttacta tgctgaattt caatataaaa ttattcttaa atctatagag
    46141 gaatttgaaa aagaacttga tgatgaacat gaaattgcac taaaattgac taactttgga
    46201 aaagatgtct taatgattgt tgaagaagta ggttatcata acccttgtct aattcactac
    46261 tacggcatag taaatggagt ttattctgaa attcttcaac acacatcaca gataaacttt
    46321 atgataactt cagtaaagaa aactgaccct tcaaaacctg ctagaagaat aggttttata
    46381 ttgtaattat cattcttatt ttctgattct ttctttagta atttcactaa tttttctttt
    46441 ttcactttta ttcatctcat gtaaatacaa aactatacat tggtacatat aatctataaa
    46501 atcactattt gattgagctg ttatctgact aaataactgt gcttgcaatt gattttcaag
    46561 catggttatt cttttctcta tttcttctat tttcttattt tccaattcaa tctctccttt
    46621 ctaaaatatt ctgtatttag ttttcaaagt actaacctaa tattgaagtt tgataactat
    46681 atcctttttc tatatctggt aatatgttat ttgttttcaa taaatcatat aaaaacaatc
    46741 ttcccttttg agtccactta gttgtcattt ttacatcagg cattccatta cttctagtta
    46801 tgtctattgt ttctgaatga gtgtatccct tcccttgatg ttgtttatat aaaagccatt
    46861 gtccactttg tttatattga atccctcttt catgaagtat tttattcatt tcttttccac
    46921 tcattccata gtcttttgct atttgagtta ttgtgacaag tcctttattt tttaatatca
    46981 tatctgtata atctgccttt ggttttagtt cttttattac ttggtctttc attttacttt
    47041 ctagttgtaa ctgctcattt acttctactt gctctataag atgttgtaac gcttctttat
    47101 atgtagttgg tagtttagtt tgcaaatcta tcatattata tgtcccagta ctacgtatac
    47161 ttggtaatat ctcattagtt acccaacgtt taaataattt tgcacttggc aacttagaac
    47221 ttaatattaa agaataaaga ccactttcat taattacttt catattttga ttaccaccag
    47281 gagtcggtat ttcacctact cctttatctt catcatcaac atgtcttttc aaagcatcag
    47341 atgtatcttt atatcctaat tgttctgcaa tatcttttcc aacaaaccaa aattctccat
    47401 ttagttcgat tactcttatt tctccaaaag tcttattttt gaatatttgt aaattattca
    47461 taatttcttt cctccttatg attgtttttg tgaacaataa attttaattt atgttaacct
    47521 tttttctaaa aaaagttcat ccattgtttt atttaaaata tagctaatca ttatcatttc
    47581 tttttccgta aaatttcttt ttccattttc ttttagatta tatgttgatt ttgctattcc
    47641 tatcttatat gctagttctt cttgggtata tcctgcttct attcgaccag cttttaaact
    47701 tcttttcttc atgttcttca ccttccaatg ttttattttt gtgaacttca tacttttatt
    47761 atattcacac aaacaaaact tgtcaataca taagtttatt tttttgttaa cttttttatt
    47821 tttttctaaa cataagttta cgtttctgta aacttatagt atcatttaat tattgggagg
    47881 tgttgtttgt ggcaactttt ggagaacgtt tcaaacaatt acgagaagag aaaaaactta
    47941 ctcaagatga actagtttca aaattcaata aagtttactt tacaagtttt aataaatcaa
    48001 caatatcgca atatgaaaat aataaaagaa aaccagaaat aaatattcta gaaaattggg
    48061 cagatttttt tgatgtatca attgattatc ttttgggtag aactcttgtt agaaatcaca
    48121 tcgatactgt agccgcacac agagttaatc ctcacaaaga tttaccagag gaagctcaag
    48181 aacaactcaa cgattatatt gaatttttaa tgaataaata taaaaaataa ggattgaatc
    48241 tatgttaata ttaaaaaaat tgagaactga aaaaggaatt tctttagata aattaagcac
    48301 agacttaaat ataaataaat ccactttatc cagaattgaa aatgggttaa gagaaccaaa
    48361 agaatcattt attaaagatt gctcagacta ttttggagta tctacagatt acttaattgg
    48421 aaaaattaac atagatgatt caaataaatt aacaaaatgc ttaaattcaa cttttccaat
    48481 aaggttaaaa gaattaagaa agaaaaaaga attaactcaa gcagaattat caaaattact
    48541 taattgttca ctaagcaaaa ttgctatgtt agaaactagc aaaagagaac ctgttaaaga
    48601 agacttgtta agaatttcag aattttttaa tgtgtcagtt gattatctac ttggtaaaac
    48661 ctcaattgaa aattacataa ctactgatga aatatccaaa ataattaaat cttatgaaag
    48721 tttacctaaa gaagcgcaag aacatattaa tagctacata gagtttttag ttgatagata
    48781 taaaaaatga atatttagag cagttcactc ctgctcttta tatataaaaa agtaacacat
    48841 acattctttt tataggggga tttcaatgaa caaactagac gcacttttag acttagcaaa
    48901 taatgaagag atagaaattt actacactga caaaatagca gatgacataa aaggattgta
    48961 tataaacaga caaggactaa aaattatatc attacttaat tcattaaaac aaaacaatgc
    49021 taaactaata gaaatcttag cagaagaatt aggacatcat tttaccagtg ttggaaacta
    49081 tgtatcttca aaaaacagtt acaaaaataa aatcttgata gacaaaactg aaaacaaagc
    49141 actaaaatgg gcatgtgaat ttcttataac agaagaagaa ataatacatg ttattaattc
    49201 acacgctaca agtgtatacg aaatagctga agaattacaa gttagcatca acttcttact
    49261 aaaaagatta gaatttctat caaaaaagaa aagcatgttg gacttaggaa ataatagatt
    49321 tttagtatta actaatttgc caaatttcta catatatgag gatatttttt aaactcattt
    49381 attctacttt tatagatttt ttacttaata aatatatatt tcaatattat tataataaac
    49441 tacacataaa agctaaaaaa ttgtaagaat attaagaaaa taattaagta aaaaccagat
    49501 aaacaaaatt aagataatat tgtacataac aaaagtatat aaagagcagt taatctgctc
    49561 ttttatataa acaccaaaca tacatacatt ctaaaaggga gagatactat tatgaaaggt
    49621 ggagtaagaa aaagaagcaa caaatggtat tactactttg acctaggcat agtatctgaa
    49681 taaagcaata tcagcttacc ttttggtaca aaaaatattt cttacttaac aacactcaaa
    49741 aaaattacca aataaatata gaaaactaaa attaagtatt tataaagtaa aaccattata
    49801 cacagtagta attttgatga gtaaacacat attccatatg taataaagct tgaagttaaa
    49861 agtggaataa ataaaatcta gaaaaaattt ttattttaag ttaagcttat tatggataat
    49921 aaaattcttg caaatttaga atcaaatgat acaataagaa tataaaatac ttgtttgaga
    49981 ggattatttt cgtgattaaa aattttgatg aatggaaaga gtatgatggt ttttcttatg
    50041 gtagtggaag aagcgagaaa atctggctaa ttaatgaaaa aactaaagat ataggcttgt
    50101 ttaaattccc taaaacaaaa gaaactggag aatattgggc agaaaaatta gcctgtgaaa
    50161 tagctagttt gttaaagatt ccttgtgcta atgttgagat aggtacatat aataaaagaa
    50221 ttggttcaat gagttattta atcaataaaa atgatgagaa attgattgaa ggcgtgtggt
    50281 taataaacaa atattatcct tgttataata cagataagtt atatgattat aaaaataatg
    50341 aatactattc attgaaaatg atttttaaat ctatagaaga atataacata aaaaatgatt
    50401 ttctaaaaat gttaattttt gattacttga ttggaaatag agataggcat caaaataatt
    50461 gggctctact agaaagtcaa aataaaatta ctcttagtcc tctatatgat aacggatcat
    50521 cattatgttg ttatgttaat attgagaaca tagattcttt cttcactgat catcaaagat
    50581 ttgaagctct tataaataca cgttcaagat caagaataag gatagatgga aaaaataaaa
    50641 aagaaccaac acacttagaa gtgctaaaat atttaaattc aaattattat gaacaaacta
    50701 tagattttgt agaaaagata ataaatctta tgaatgaagt aaacataaat acattaatag
    50761 ataactattc agaaaatatt ataagcccaa agttaaaaaa attgctaaaa atatatctaa
    50821 aagaaaaagt gttaagaata aaaaatatat ataatttgtg aaagagggat aaaatgtcaa
    50881 ttaaaaatgg aaaagatttt ttatatgtta tttggaaaga tccaaaaagt agaagacaat
    50941 ttataattgg aaagttagct aaaaatggat tgtatgaatt ttcttatgaa tatgaaattg
    51001 atgaagctat taaagcaggt tttaatgctt taatatcatt tgatgatatt agtaaaacat
    51061 attatagtga agatttattt agcccatttg caagtagatt acctgataaa aaaagaaagg
    51121 acatcaataa aattttggat aagtatgata tgtctgagta tgatacatat gaacttttaa
    51181 aaagaagtgg tgcaagatta cctatagata cactagaatt tgtagatcca attcctttgg
    51241 acattgataa aaatataatt agatattttt atattgcagg agtaagacat tatgctgggt
    51301 gtgaaggaca tgaatgtgat aattcaatag aattagaatc aaatgaagct ctagttctag
    51361 agttagaacc agaaaataaa tttgatcctc atgcaatcaa ggtatttgat aaaaaagaga
    51421 atcatatagg ttacattcct agatattata gtggagaatt aacaagtata ataaatagag
    51481 gattaaagta taattgcatt attaaatttg ttaacaaaga aaataattgt gatgaatgta
    51541 taaaagttaa attagaaata ttatttgacg aatataatta atgtcttctt gtaaatatag
    51601 gagacatttt ttatataaac accaaacaaa catacattct aaaagggagg gatactatta
    51661 tgaaaggtgg agtaagaaaa agaagcaaca aatggtatta ctactttgac ctaggcatag
    51721 tagaaggaaa aagaaaaaaa gtagaaagag ttggtggcaa tacaaaaaaa gaagcagaaa
    51781 aagcactaag agaagcacta aatgaatatg aaaactctgg catagtcttt gaagaaagta
    51841 acatcagctt atcagactac ctagactttt ggtacaaaga atatgtttta ctcaactgta
    51901 aatacaacac tcaagaaagc taccgaataa acatagaaaa acatataaag ccaaaactag
    51961 gagcttacaa agtaaaagct ttaactcctg caatactaca aaacttcata aataaaaagt
    52021 acaaagagga ttattctcaa aatacattac aagtattaaa agccatatta cataggtcat
    52081 taaaatcagc agtccatcct tacaaacaca tacgagaaaa ccctatgcaa tatgtaagca
    52141 taccaaaaac taaatctaaa acagaaacta ataaagttaa aactattaca ttagaagaat
    52201 ttaatcaaat actaaatata tttcctcaag attcatttca acgtatagtt ttactaattg
    52261 gatttcatac tggtatgcga agaggtgaaa ttattgcact aaaatgggat aatatagacc
    52321 ttgataataa aactatcaca gtaaagcata ctttgattaa aaaaccaaat ggaatgtttg
    52381 aattagggca accaaaaaca gaaagctctt gcagaactat atttacaggt gacactttaa
    52441 taaaggcatt aaaagaacat aaattatatc aaaagaaaat gaaattaaaa tacggagaat
    52501 tttactttga tagtgattgg gtatgtacca aagaaaatgg tcaacaagtg aatactcaca
    52561 ctttagacac tatagtaaga caaattcgag tagctttaaa caatgacttc cattttcatt
    52621 ctttaagaca tgcacatgct actctattat tagaaaatgg tgctaacatt aaagacatac
    52681 aaaaccgttt gggtcatagc caattatcaa ctacaatgga tacctattca catgtaactg
    52741 ataaaatgaa aaatgaaact gtagatatat ttgaaaaaat tacaaattag agtttgccac
    52801 ccaaaaatat aatacggtgg caaatgggtg gcaaaatcta atttatctat tttaaaagct
    52861 aaaattatca aatttatata gtcagctata cgcttgtaat ttcaaggctt tagagtatat
    52921 aacaaccata actaatataa ggtattaata ataaatctaa cgaataaaac ttaatattta
    52981 cttaagttgt aatactattc taaattgtat aattaaaatt taataagttc ttaattatat
    53041 attcattgat ttatttgcaa aaataaaaaa tgccaatcta tctctaaaca aaaactgaat
    53101 accaataaaa atttaataaa aaattttatc aatattcagt ataagtttta gttacatatc
    53161 aaattttcca atgcatcatt aggtgctaca ataggataaa catcataatc cttatctata
    53221 tcacattgta aaaatacagg tttattaaaa tctatcacat ctagaacctt tcctaattct
    53281 tccatattgg ataccttata tccatcaata ttatatgcat ttacgagttt aacataatcc
    53341 acattttcat taatatcagt ttgagagtat ctttgattgg aaaataactt ctgccattgt
    53401 cttaccattc ctaatgttct attattgagt agtaaaataa gcataggtac attataattt
    53461 gcaactgtag ctagttcatt acagttcatt ctaaaacttc catcaccagt tactaaaacc
    53521 acatttttat ctacattacc agctttagtt ccaatagctg cacctaaacc aaatcccata
    53581 gttcctaacc ctgctgatgt tataaaactt ttattacctt taaaattcca gtatttagca
    53641 gaccacattt gatgttgacc gacatcagtt actaccacag tgggtttctt taatgtttca
    53701 tatttttcat ttattttttt aagtatattt tgtggatgaa attcatatgt ttgtacaccc
    53761 tcactctttc taaatgtctt tatctcttct ttccaattgc tattattctt actttcaacc
    53821 ctttcaatta ataaactaag gactagcttt acatctccaa ccaaagatac attagattct
    53881 atatttttac ttatttcaga tgggtctatg tctatgtgta ttatttttgc atttttagca
    53941 aattcagaac ttttacttat cactctatca ctaaatctcg cacctatcgc tattactaaa
    54001 tctgaattag aaagtgctaa attactttct ctacttccat gcattccaac cattccaaga
    54061 gatagttcat tctttctatc tatatttcca agccccataa gtgtatttag tacgggtgta
    54121 tctatcttag ttgcaaattt ttcaagaatt tcctcactgt ctgaagattt aacacctcca
    54181 cctgcatata ttacaggctt tttagattct tttatgatat ctattgcttc atttaaaagc
    54241 ttaatattag tttcatcatc tagatcaaaa tcactcttat aatccatata atcatctatt
    54301 tgacataaat catagtcctc accactaaaa tccatttctg ctaaaaataa atcttttgga
    54361 acatctacaa gtacaggtcc ttttctacct gaatttgcaa ctctaaaagc ttcttttatt
    54421 gtaggaacta attctttagt gtttctaact aaataattat gttttgtcat agataatgtt
    54481 gctccagtta tgtcaatttc ttgaaaagaa tcttttccta ataaactagt tggcacctgc
    54541 cctgaaatta caaccatagg tgaagaatcc ataaaagctg ttgctattcc agttatggca
    54601 tttgttgctc ctggaccaga tgttgtaaaa caaactccta ctgtattagt acttcttgcg
    54661 tacccatctg cagcatgaac taaaccttgt tcatgggatg tccttatatg tttaaaatca
    54721 tctgaataat cgtatagagc atcataaaga ggtatcacgg ctccaccagg atatccaaat
    54781 attgtgtcaa ttccctcttt ctttaaacat tctaaaatta cctttgctcc attcatacgc
    54841 actactatac accactcttt ccctcaaact aatccattta atatatattt attttaagaa
    54901 tgacatcata cttcttagat ttctaccaac ttcggcaatt tctgtattat attcttcttc
    54961 tctagttttt aggaagtttt cacgtccttc ttcattttct ttaatccacg cttttgcaaa
    55021 ttttccattt tgtatatcct ctaaaacatt ttgcatacct tgttttgcag catcagttat
    55081 taccctttta ccagatacat aatctccata ttctgctgtg tcacttatac tatatctcat
    55141 tctttcaaat ccaccttcat atataaggtc gactattagt ttcatttcat gtaaacattc
    55201 aaagtatgct acttcttttt gataacctgc atctacaagc gttttatatc ccaattttat
    55261 aagttcactt attccaccac aaagtactga ttgttctcca aataaatctg tttctgtttc
    55321 ttctttaaaa gtagtttcta aaacacctgc tcttgttgct cctatccctt ttgcatatgc
    55381 taaaactgtc tctgtagctt ttttagtgta gtcttgatat actgcaaata gtgcaggaac
    55441 tccacttccc tttgtaaata catttctaac taagtgacca ggtcctttag gagctaccat
    55501 tacaacatca acaaattctg gtggttgtac ttgattataa tgtatattga atccatgagc
    55561 aaaagctaat gtctgacctt cttttaagtt atcctttaca ctctcttcat aaactttctt
    55621 ttgtttttca tcaggcatta acatcattgt caaatcactt tcttttgtag cttctgctac
    55681 actcttaact tcaaatccat cttcttttgc tttttgtgct gatttacttc cgtcataaag
    55741 acctatcact acatgtacac cgttatctct aagattttgt gcatgtgcat gtccttgact
    55801 tccataacct aaaactgcaa cctttttatt ttttaaaacc tctaaatcca catctttttc
    55861 ataatacatt cttgccattt tcatttcctc ccaatacttt ttcttatttt tatgatattt
    55921 atttttatta gaaattcata ctcattatca ttgttttaat attattcaac agatttaaat
    55981 tctaaattta tataatctta caattataat tttagaattt tcagttttta ttagctacaa
    56041 caaactaata aatttattaa ctgtgataaa ctgataaatt caatgacact tatattttaa
    56101 tctaagattt ttcttattta atatttatct ttagctttat aaaatatatt taattaatgt
    56161 ctcattcttt caacagttat ctctcttata tcatgtagtt ttttcatatg gtttaagaca
    56221 gaatctaaag atgtcttttc ttcatttact attaatttta tacctgaatt attcatatta
    56281 tctacagtca tacttattga ttgtatatca acctctcttc ttcttagagt catagccact
    56341 cttaaaaaag aatctattcc ttggtttaat ttagcatata cagtattttc cataaaaatc
    56401 cctccttatt tttattattt ttttattaat tttatatgga ttaaatttat ttatatcgct
    56461 taaaataaaa aaactttcat ctttataagc aatataaata ttacttataa ggacgaaagt
    56521 tatattccgc ggtaccacct tatttgtaga tataaaaaat acatctaccc ctcttttttc
    56581 aggtcgggta attaattacc ttaaccttag tcatgtaacg ttgacatacg tttgtactta
    56641 ctaaaaatat tcagtacaaa agctccagag tgatcattat actactgtta acaccaattc
    56701 tcagcaacat tggttctctg tggataacac tattagtatt gttgtctctg tcattgcttt
    56761 taatttttta attttatatt ttaattctgt tttgttgtta atatagatta tattccctat
    56821 catttacaat gtcaatgact ttttttaaaa ttttcagatt atttattttt ttcacaataa
    56881 tacttctttt atactttacg cctgtatttt ggaataatcg ttttcagatt attattttat
    56941 atccatttat ttttagccaa ttttctttat ctttataatc gggcataatg gattttacta
    57001 aatcatagaa ttttttagag tgattcatat gtactaaatg gctaaactca tgtattatta
    57061 cataatcaat tacatctaat cttgccatag atatttttga gtttatataa atattttttt
    57121 gagctgtaca acttccccat ctcttatttt gttcttttac cttaacactt gctggaacta
    57181 attgcttcat tatttcacaa tttaatttaa gaaaatctat tctctctaat actgttcttt
    57241 cactttccat cttataccaa gattttaaag attttttaat aaaatctgta cttgtatcat
    57301 ttgtctgaac aactatttga tattcgttaa tagttattct acagttactt tttttgacag
    57361 aatctttcaa cagttctttt ataactagat ggtactcttc acctaaataa agaaattttt
    57421 cgccagttat aaacattctt tgtttgtata cttcttctct tcctttatat ttttctgact
    57481 ttttaagtat ccagtctcct ttttcaataa ttatatcttt tattattttt ttggatattt
    57541 taggaggact cattacaatt attttgccaa atggatctat ttttatagaa atattttttc
    57601 tatttttata tactaaaact gcttttattt caatccctct aaaattaaca tttaaagtat
    57661 caatatcttt actccctgtg ttcattagtt tatttttcct tattagctct acaatttata
    57721 agttcagcag ctatacatac agctatttca gctggtgttt gagcattcaa ttttatgcct
    57781 attggtgcat gtacttttga taattcttcc ttcttaaaac cttttttcat gagtttttcg
    57841 tacgtcaatc caatttttgt tctacttcca atcataccta tataaccagt ttctctttta
    57901 agagcccatt ctaaagcact tgaatcatat agatgacctc ttgttaaaat aattatatag
    57961 ctattttcgt cagttggaaa atctggtata ttttcaaaag aatctaaaac tactacttct
    58021 gattctggga acctttcata attagcaaat tcttctctat catctattac tactgttcta
    58081 aattctaaaa gagatagtat cacagcaaca tctcttgaaa tatgacctgc accaaatata
    58141 tatgctttac ttgtattttc ctttagtctc ttattgaatt tttctatatt actttttaat
    58201 ttactatcta taaaatctat ttgtatttct ccagtacctc cacatatcat tcctagtttt
    58261 gccacatcag atttgtttag agtaaattta tacaacattg attctctatt ttttataaca
    58321 ttaattgcat gttttataca ttctgcttct acactacctc ctcctactga accaaatata
    58381 gtagaatcat ttttcacaat catcattgct ccagtttcac gtggtgttga acctgaatga
    58441 gacgttattg tagcaaatgc gaaatcttca ttattttcta ttaatcttat tgcttgttca
    58501 tatatattca tataattctc cttaccagtc tatctaaaaa tcttctatat atctatctta
    58561 gtctttttat tactttattt ggtatcacaa attctactgc tcccatataa ccaggtacta
    58621 cttcatattc ttcaaaacta atcacaagat ttccattttt attaaaataa aaatcttgat
    58681 acttatttat agattcaaaa tctttcactg ggaagtcttt atcttggtct ataaaataag
    58741 attttgtatc atctttctta atttgatttc tcatctgctc ttttatatta tcacttatag
    58801 ctctaatata tctactatct ttaaaaaata ttggtaaatt taatagtatt cctctatttt
    58861 tatcaacagt gtaatgcttc ttagttatag acgaggaatc ttctatctct tcttttgtca
    58921 tttctattga taaaaagtcc ttagaattat ttttgacatt atggattaca tttaggtatc
    58981 tatgaccctt attattttcc tttttcattt tattttgaaa ttcactgtaa atttttttcc
    59041 catcttgcaa aaactcattg tttaatttat tctctaaagt tttatctttt aaacctttta
    59101 ttttaggaat actcacattc acttcataat tgccttgttt aaagttgaga ctcttagcat
    59161 ttaacacctt tatcgtatca ctatttgaag tctcattaga ggaactacct gcaaatatag
    59221 ggtttatatc tactaatgag actaataagc atattgaaaa tatagaaatt ttaaatatag
    59281 attttttaag ctttttatat gtagtcatat tgtccttctt aaaatatgta tatataaact
    59341 actattcaat ttacaaatct tttattactt tatatggtat tacaaactca actgcaccca
    59401 tataaccagg tgctacttca tattcatcaa aacaaattac aaggtcttca tttttattaa
    59461 aataaaaatc ctgatactta ttaattgttt taaaatcttc aacaggcaag tctttttttt
    59521 gatctataaa ataggatttt gtactatcct ttttcatttg ttctctcatt tgttctttta
    59581 tattatcact tataactttt atatacttat catctttaaa aagcattggc aaagtcaaaa
    59641 ctatttgtct ttttttatca atagtataat gtgccttact cacatatgat gaagcctcta
    59701 tctcattttt tgtcatctct attgatagaa attttttaga attatttttt acactataac
    59761 tcaaacttaa agatttatat cctttattct ttgatgattg tatcttctcc attctctctt
    59821 ggaattgatg ataaagcctt tttccatctt ccataaaact actgtttaat ctttgttcta
    59881 agtttttatc ttttaaacct cgtattttag gaatatcaat ctcagcttca taattttcac
    59941 tttttaaact ataatttttt atagttatta cttctactat tactccaatt acaggtatct
    60001 tagaaaaatt gtctgcaaat acaggactta tatttactaa tactgtaaat atacatgcaa
    60061 aggctattgc aactttaaat cttaatctat ttactttttt atttttctct cgatatatgt
    60121 tttctatttt ttcatgattt attatagtat ctaatttctt gggtattgga attttatgat
    60181 actcatcttt taacttattt aacctttctt tgcttgtcat tctttttatc cctactcttc
    60241 aatttttaat tttagttttt ttaaagcagt atataaccta gttttaacag tattcacatt
    60301 ttcatccaat attattgcta cttcttcaat ctttaaatct tcaaaaaatc ttaatattat
    60361 aatagatttg tattcaatag gtaattcttc caaagctctt cttaaatcca aatcagtata
    60421 tttatcatac tctccagtct catctataag gtcaatatct gtcatattat tatatttacg
    60481 gttttttcta ataaaatcta tactcgttct aactaaaatt ttataaaacc atgtctttat
    60541 ataatttact tctttaatat tttccactga gtatagtgcc ttatacatgg cttcttgaac
    60601 cacatctaag gcatcttctt cattcttcgt ataactatat gctattctat aaaaagagtc
    60661 tttattatcg ataatgtatt tctcaacttt cgttctcttt aaactcttaa acattcctgt
    60721 aaaatctccc tccgattaca ggctaggatt gagctgttag aaattctatc tgtctatttt
    60781 agcatcttca ttcagttttt ttatatgctc aatatacttt tcatttaaaa tagattctct
    60841 tattaaactc tgacactcat taaaactttt ttgtctttct cttttatcag taactttgac
    60901 tatatgatat ccataactag tttcaaacac atttgaaact tcatttttct ttaatttgaa
    60961 cacttccttt gtaaactcag catttttatc atcctttgta aaatatccta gctgtcctcc
    61021 attttttcca gttgctttat catccgaata ttttttagct aaactttcaa aactttctcc
    61081 atttttgatt ttagtcaata tattatctgc cttcttcttc aaagcttctt ttttatcttt
    61141 gcttacttct tttttatttt tatctaaagt cgatataagt atctgagaag cacttacttc
    61201 ttctacatta aaatcttttt tatgacttgt atagtaggct tccatatcat tatcacttat
    61261 gtttattcta tcttcaaagg cctttttatt tttatctatt gctaaatctc tagatatttc
    61321 ttgttttaaa tattctttat caacacctgc tttgtccatt ttttccttat agcttgtatt
    61381 caaattcatc ttatcttcta gttcttgata ttttgagtct acttcacttt tacttggagt
    61441 tagtccttta tccttagcct cttgatataa aagttcatta tccaccataa acgatattat
    61501 aacattttct aatttatttc tacctttttt atccaaagtt ttttctaatt catctaaaga
    61561 ctgcccattt atataattat tagtagcaga taaaactgct tttgtttttt tgtactgttc
    61621 ttttgtgatt tccacatcat ttacttttgc taaactttta ttacaaccaa ccatcatcaa
    61681 agacaatatt ccgaccattc ctatataaag ttttttattc aatataccta cacctcactc
    61741 atttttattc tcaatttcta aaaatatcaa tcaatattct tatattttaa ctactctaaa
    61801 ttacgtaacc aataattttt tggtatttgg ttttgatcta ctggcgcaaa ttcataacct
    61861 tcatctgata agaattttaa aactgaatct aaagcttctg cactctgttt tttctcatgc
    61921 attaaaacta caagttggtc tttatcttca ccttttctat tcttaatatg attttttaca
    61981 ttttgtacaa tttgagaaga attagatctc caatcttctg tatctaagtc ccagtcccaa
    62041 attttatatc cagcatcaac taaagcctga taagaggctt gtggtgtata tggtttgctt
    62101 ccataaggta ttcttattac ttttgaatgt ttaccagtta tcttataaaa agtttgatca
    62161 tttgtatcaa attcctcttt tgctgatgta ctagtaacat aaagcttgtg aatatcatgt
    62221 gatacactat gaaaacctgc tgtgttccca tttttaatta tatttttaac ttgctgtgga
    62281 tattccttca tattcccatc tatcataaaa aaagttgctt taacattgta tttatttaaa
    62341 gtttttatca tctcgtcagt aaatttagat gggccatcat ctatagttat gtatgctatt
    62401 tttccagaac ttttacttgg ccctttacta acttctattg atgtattaga tactatatct
    62461 gtaatagtag aacttgacag ttttgcattt ctactaattt cattttcctt catatttaaa
    62521 gagctgacgc gaacaccaac tactataaaa gccagacaca ctacaatagc acttaaaagc
    62581 aaatacaact tctttctatt tatttttttc tttttatttc tcatcgttcg acctccaata
    62641 ttactatatt tttacattta tatttttgta gtattttatc ccaaaaattt acactacata
    62701 tatatagacg ttgaacttcg acaaaaagtt ttgcttaatt tttaaaattt gttatttcgg
    62761 tgattattat gctgtataat ttctgtataa ttgtat
//
LOCUS       DAELXX010000007        59105 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.007, whole genome shotgun sequence.
ACCESSION   DAELXX010000007 DAELXX010000000
VERSION     DAELXX010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 59105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59105
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.007"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..964
                     /locus_tag="KPH29_000446"
     CDS             <1..964
                     /locus_tag="KPH29_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protease"
                     /protein_id="HBG1466086.1"
                     /translation="LLLFLKRYFFKRFSKRFNYYIWLVIVFRMLLFLFNYTVMYEVKE
                     PKENTVGNNITQIDVSIDNDFMLYLVYLWLFVTIAIAVYTFMEYTKFKNLVVDVSYDI
                     EDHDVNCIYKNLLKELDIKKKIELRGSDELISPAGMGLFKSYIFLPDYTYSKDELTWI
                     LKHELMHFKNKDILIKFLVLSVKIMYWFNPLVYIMSNKVNLDCELCCDESVLTDCSLK
                     DKKAYALALIKSIKLSKNYNSGILTTEFNKTNLEKRLDSIIKKKGKSGILMLLILFTL
                     FSVTYFNFESISFDNSNYLRNIGILEKTGADLKEEDDISMRVVN"
     gene            1443..1616
                     /locus_tag="KPH29_000447"
     CDS             1443..1616
                     /locus_tag="KPH29_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HBG1466087.1"
                     /translation="MHLVQLLCQQINGSDIDPDSKFGPATYNQVKILQGRLGVTKDGV
                     VGKATWQAVGERY"
     gene            complement(1701..2723)
                     /locus_tag="KPH29_000448"
     CDS             complement(1701..2723)
                     /locus_tag="KPH29_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBG1466088.1"
                     /translation="MNYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTLLNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNSGNGIVFIKPWNNSKIIEKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTKLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKVRNELCETMILTGCQNVKDIDGR
                     VIWNLL"
     gene            complement(3054..3236)
                     /locus_tag="KPH29_000449"
     CDS             complement(3054..3236)
                     /locus_tag="KPH29_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466089.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMVKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            3549..3968
                     /locus_tag="KPH29_000450"
     CDS             3549..3968
                     /locus_tag="KPH29_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HBG1466090.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIVQIENCIV"
     gene            4099..5940
                     /locus_tag="KPH29_000451"
     CDS             4099..5940
                     /locus_tag="KPH29_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG1466091.1"
                     /translation="MEEIDDTILDSMFKLTLNERLLTNNDFVENKIGVVKNLIDCYKT
                     EQNSLMLLKSNFLLALLYGIQGFSEKMKEYILISCKYIDKCLPKDYKFLARFYSQIAI
                     LSLKNEDVNKANLYMDKFNLICDENNFLIEEIIFKSQLIYIKASKCIESSVIIKEIET
                     LYIKIKEINDFNCKKIYFFIIGKIYFLFLDDALIAKVNFLKARKYADLLKDMQVSSLC
                     NIKIGECECSFENYECSIEYLNEVISNKKYRNVNIIQKYRASNNITKILIKTRNYPVA
                     INNLAKSEVYLEKIKNQSLKEVEKFDLFISLAMYYAKSNEKSFEQSICYLDRAKNILN
                     MIKNIEEYTIYDLEIIYHQAKIYYIFGYYDNALNTNKKLLEKSKNAKNYNYVKLAYKN
                     IYLCFEKIQNYEMSMKYFKMYYELKMMYIKARNRNYIDSLNYRHEYIETEINNMRKVK
                     LDLDKKKYVDHLTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFKEYNDTYGHYNGDI
                     ALKNITIIIKKYLKKDMFMIRYGGEEFLILSVCKDYKKSKIFGRKLCRIVKKFLNNNL
                     TVSIGIDTCKNSSIDIREIIENADKALYKAKESGRNRCLHYHDFKNF"
     gene            5967..7853
                     /locus_tag="KPH29_000452"
     CDS             5967..7853
                     /locus_tag="KPH29_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG1466092.1"
                     /translation="MKRSLIEDLKRNINKIEYDMLTKFYLNLTDLNYILDYHKDLLNT
                     KLEDNSRVYTLYLISLINYIKKDREASYNYLIESLKYIKKLSLKEDSDIISKIYIYLT
                     LSAISIRKYDKANNFYFSAKKVIRQNHLNELLVLLNMSLCVEISALYQTKTDVIALVE
                     EVSFFKTLKNKALVSRANYIFGRVYLYLFNNFIKSMEYLIECLKLAQENNLYSIEAFC
                     KCIISLCYLESGNNVEAIKYINNVLDVAHSKNSISVTERVSIKIDLIWAYLKEDMNKE
                     AEVILLKTIKLMKVVEGVYKDYLYSFIFLYSAEIELRKDNCSFEKVNTYLILSKNIYK
                     SFGDVFIYNSKLDYIYKLYGDLYIKFGNIDEGISCYIKGLNLVKKSSLNLRKISIFYG
                     LIANGYELKKDFKLAIEYYKSMDWYSDRWEKNNSYKTSQAIHKQYELRQKQESLRLLV
                     DDNKKLENDILKDGLTKLYNRRYLEQQLDLFNGTRDKNMVAILVDIDCFKSYNDNYGH
                     LAGDKVIVKVTDIIRSVFIDITEHIIRYGGEEILILVDSIESIGVEEIDNIELYIKRV
                     FKLLEFEGIEHIYSKIKNYITISAGVSIKRCRSRKDVEILIEDADKNLYKSKKAGRNQ
                     YIII"
     gene            7861..9861
                     /locus_tag="KPH29_000453"
     CDS             7861..9861
                     /locus_tag="KPH29_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG1466093.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEVVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTREFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            10123..11382
                     /locus_tag="KPH29_000454"
     CDS             10123..11382
                     /locus_tag="KPH29_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG1466094.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVEEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            11443..13230
                     /gene="iorA"
                     /locus_tag="KPH29_000455"
     CDS             11443..13230
                     /gene="iorA"
                     /locus_tag="KPH29_000455"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HBG1466095.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEMNDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            13232..13807
                     /locus_tag="KPH29_000456"
     CDS             13232..13807
                     /locus_tag="KPH29_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HBG1466096.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            13860..14930
                     /gene="buk"
                     /locus_tag="KPH29_000457"
     CDS             13860..14930
                     /gene="buk"
                     /locus_tag="KPH29_000457"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBG1466097.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            15148..15849
                     /locus_tag="KPH29_000458"
     CDS             15148..15849
                     /locus_tag="KPH29_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466098.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHASN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKRSN
                     EHKCKSSIVIKNEQN"
     gene            15908..16414
                     /locus_tag="KPH29_000459"
     CDS             15908..16414
                     /locus_tag="KPH29_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBG1466099.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEMYELLYE
                     IDNNKLNI"
     gene            16589..17611
                     /locus_tag="KPH29_000460"
     CDS             16589..17611
                     /locus_tag="KPH29_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG1466100.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(17758..18066)
                     /locus_tag="KPH29_000461"
     CDS             complement(17758..18066)
                     /locus_tag="KPH29_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBG1466101.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(18126..18554)
                     /locus_tag="KPH29_000462"
     CDS             complement(18126..18554)
                     /locus_tag="KPH29_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466102.1"
                     /translation="MNNKLHELEKNLPETSCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            complement(18777..20234)
                     /locus_tag="KPH29_000463"
     CDS             complement(18777..20234)
                     /locus_tag="KPH29_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBG1466103.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            20652..21566
                     /gene="nadA"
                     /locus_tag="KPH29_000464"
     CDS             20652..21566
                     /gene="nadA"
                     /locus_tag="KPH29_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBG1466104.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKLLSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            21579..22880
                     /locus_tag="KPH29_000465"
     CDS             21579..22880
                     /locus_tag="KPH29_000465"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HBG1466105.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            22861..23697
                     /gene="nadC"
                     /locus_tag="KPH29_000466"
     CDS             22861..23697
                     /gene="nadC"
                     /locus_tag="KPH29_000466"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBG1466106.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHIGAAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
     gene            23953..24285
                     /locus_tag="KPH29_000467"
     CDS             23953..24285
                     /locus_tag="KPH29_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBG1466107.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            24447..24632
                     /locus_tag="KPH29_000468"
     CDS             24447..24632
                     /locus_tag="KPH29_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466108.1"
                     /translation="MSEKYADKLEIKLYQAGKDFSYIKKYGIITKGTLIINQKKKYDR
                     LNKDTIERAIVEAINNN"
     gene            24659..25744
                     /locus_tag="KPH29_000469"
     CDS             24659..25744
                     /locus_tag="KPH29_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBG1466109.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLYNDSEEEVIEL
                     EEEKRSFMENMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSTIPKMKEVIGNHA"
     gene            25737..26195
                     /locus_tag="KPH29_000470"
     CDS             25737..26195
                     /locus_tag="KPH29_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466110.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            26230..27321
                     /locus_tag="KPH29_000471"
     CDS             26230..27321
                     /locus_tag="KPH29_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG1466111.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKAGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            27326..27550
                     /locus_tag="KPH29_000472"
     CDS             27326..27550
                     /locus_tag="KPH29_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466112.1"
                     /translation="MENIKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTS
                     GFVINYMVNRDYFENIKVSDIMDIFTINDC"
     gene            27560..28123
                     /locus_tag="KPH29_000473"
     CDS             27560..28123
                     /locus_tag="KPH29_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466113.1"
                     /translation="MQWALSSEEIDIAIICKDAAKQYVNVNSGFEIVGTVVQNSDIFL
                     LSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAYGLESNLVDGVVIDCIKS
                     IGLEGKRKSTTKLGDYDTYVLVVSKEFKKSKAYSDFITLYNKSVDALKNKATFKKALK
                     EYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            28084..28713
                     /locus_tag="KPH29_000474"
     CDS             28084..28713
                     /locus_tag="KPH29_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1466114.1"
                     /translation="MEIKISKDKSDRLTKKKTDGSMDILYKIILLVIIFLVWHFAAKD
                     IGSSLLLPMPVDVIKGFFFCVTDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFS
                     KTFERVLSPVVDSVRQVPIMAWVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVR
                     AISKDYYNAARSMGASPIVIFTNVIVPASLPDILTGSRIAISTGWMSVI"
     gene            28753..28890
                     /locus_tag="KPH29_000475"
     CDS             28753..28890
                     /locus_tag="KPH29_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG1466115.1"
                     /translation="MVQAQTLMHTDVLIGLMIFAALIGFIIDRVLKFINKILCRWRFA
                     D"
     gene            28890..29642
                     /locus_tag="KPH29_000476"
     CDS             28890..29642
                     /locus_tag="KPH29_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1466116.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            29655..30140
                     /locus_tag="KPH29_000477"
     CDS             29655..30140
                     /locus_tag="KPH29_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466117.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYVSKK
                     LNIEKEYIERTLQYFIYENIMVVDGNSINFIYPVSALQTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     C"
     gene            30147..30296
                     /locus_tag="KPH29_000478"
     CDS             30147..30296
                     /locus_tag="KPH29_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466118.1"
                     /translation="MNFFWTKSDFDAWTNEAGLSDDEDIYCLDINEAIVESYKIFKLK
                     QKVLS"
     gene            complement(30432..31253)
                     /gene="yidA"
                     /locus_tag="KPH29_000479"
     CDS             complement(30432..31253)
                     /gene="yidA"
                     /locus_tag="KPH29_000479"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBG1466119.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMTLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNEAPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
     gene            31745..33439
                     /locus_tag="KPH29_000480"
     CDS             31745..33439
                     /locus_tag="KPH29_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBG1466120.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSHFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGNIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKISKKLGFEDFVEL
                     GYVRMMRSDYREDMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIQNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
     regulatory      33771..33881
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            33985..34356
                     /locus_tag="KPH29_000481"
     CDS             33985..34356
                     /locus_tag="KPH29_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HBG1466121.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQDNKKTPKWTENVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            34452..35399
                     /gene="trxB"
                     /locus_tag="KPH29_000482"
     CDS             34452..35399
                     /gene="trxB"
                     /locus_tag="KPH29_000482"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBG1466122.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            35484..35801
                     /locus_tag="KPH29_000483"
     CDS             35484..35801
                     /locus_tag="KPH29_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HBG1466123.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
     gene            36070..37356
                     /locus_tag="KPH29_000484"
     CDS             36070..37356
                     /locus_tag="KPH29_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBG1466124.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            37468..37941
                     /locus_tag="KPH29_000485"
     CDS             37468..37941
                     /locus_tag="KPH29_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:37600..37602,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HBG1466125.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            37973..39283
                     /gene="grdB"
                     /locus_tag="KPH29_000486"
     CDS             37973..39283
                     /gene="grdB"
                     /locus_tag="KPH29_000486"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:39020..39022,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBG1466126.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            39540..41072
                     /locus_tag="KPH29_000487"
     CDS             39540..41072
                     /locus_tag="KPH29_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="HBG1466127.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNSDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            41083..42228
                     /locus_tag="KPH29_000488"
     CDS             41083..42228
                     /locus_tag="KPH29_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HBG1466128.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
     gene            42538..43617
                     /locus_tag="KPH29_000489"
     CDS             42538..43617
                     /locus_tag="KPH29_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG1466129.1"
                     /translation="MSRVKNVVGLLGTKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATKLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            43730..44329
                     /locus_tag="KPH29_000490"
     CDS             43730..44329
                     /locus_tag="KPH29_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466130.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLPPKGQE
                     SSQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIAALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
     gene            complement(44516..45388)
                     /locus_tag="KPH29_000491"
     CDS             complement(44516..45388)
                     /locus_tag="KPH29_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG1466131.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
     gene            45805..47208
                     /locus_tag="KPH29_000492"
     CDS             45805..47208
                     /locus_tag="KPH29_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466132.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            47235..48779
                     /locus_tag="KPH29_000493"
     CDS             47235..48779
                     /locus_tag="KPH29_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBG1466133.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKAGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            48810..50201
                     /locus_tag="KPH29_000494"
     CDS             48810..50201
                     /locus_tag="KPH29_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HBG1466134.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            50394..51890
                     /gene="abfD"
                     /locus_tag="KPH29_000495"
     CDS             50394..51890
                     /gene="abfD"
                     /locus_tag="KPH29_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HBG1466135.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            52025..52300
                     /locus_tag="KPH29_000496"
     CDS             52025..52300
                     /locus_tag="KPH29_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466136.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            52324..53631
                     /locus_tag="KPH29_000497"
     CDS             52324..53631
                     /locus_tag="KPH29_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBG1466137.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVRESLAVEFEKRFKEKY"
     gene            53651..54769
                     /locus_tag="KPH29_000498"
     CDS             53651..54769
                     /locus_tag="KPH29_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HBG1466138.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
     gene            55056..55976
                     /locus_tag="KPH29_000499"
     CDS             55056..55976
                     /locus_tag="KPH29_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBG1466139.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFIPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            56091..57248
                     /locus_tag="KPH29_000500"
     CDS             56091..57248
                     /locus_tag="KPH29_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBG1466140.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(57333..58832)
                     /locus_tag="KPH29_000501"
     CDS             complement(57333..58832)
                     /locus_tag="KPH29_000501"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBG1466141.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIENEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
BASE COUNT        23101 a         6514 c        10610 g        18880 t
ORIGIN      
        1 tctattatta tttttgaaaa gatatttttt taagaggttt agtaagagat ttaattatta
       61 tatttggttg gtaatagtct ttagaatgtt actttttcta tttaattata cagtaatgta
      121 tgaagtaaaa gagcccaagg aaaatacagt aggaaataat attacacaaa ttgatgtttc
      181 tatagataat gattttatgc tgtatttagt ttatttatgg ttatttgtaa ctatagccat
      241 tgcagtttat acatttatgg aatacacaaa atttaaaaat ctcgttgttg atgtatctta
      301 tgatattgaa gatcatgatg taaattgtat ctataaaaat ttgttaaaag aattagacat
      361 aaaaaagaaa attgagttaa gaggttctga tgaattgata agtccagcag gtatgggatt
      421 atttaaatct tacatattct taccagacta tacttatagt aaagatgagt taacttggat
      481 tttgaaacat gaattaatgc attttaaaaa caaagatatt ttaataaagt ttttagtttt
      541 gtctgttaaa ataatgtatt ggtttaatcc acttgtttat ataatgagca ataaagtaaa
      601 tctagattgt gaattatgtt gtgatgaaag tgttttgact gattgctctt taaaagataa
      661 gaaagcatat gcgttagcac ttataaagtc aattaaactt agtaagaatt ataatagtgg
      721 tattttaaca actgaattta ataagacaaa tttggaaaag agacttgata gtattattaa
      781 gaaaaaaggg aaaagtggaa tattgatgtt attgattttg tttacattat tttcagttac
      841 atattttaat tttgaaagta ttagttttga taattcaaat tatttaagaa atatagggat
      901 tttagaaaaa acaggcgcag atcttaagga agaagatgat attagcatga gagttgtaaa
      961 ttaatagatt tttatagtat tataatttat taacaaatta ggagaagttt tgaaattaaa
     1021 aatctaaaaa aaatcttagt agtcacaggg agggtgactc taatatctat tagtttagta
     1081 tttgcaaata ctaataatga tgtaaatgta tgaaaaagta atgaagagca aacaacaaat
     1141 tcatttacaa taactggtta cgaatttatt tatgaaaatg taccacaacc tataaaatct
     1201 gattatgaaa aaaatgtaaa gaattaaata taaatccatc taaagatgat acaatcttta
     1261 ttaatgaaaa atttatagat tcatataata ttaatataga tgattataat attgaaccaa
     1321 aagcatttat tattagatat aaaaaagatt taaatcgttt tgatgctact ggtcctataa
     1381 gttatagcgt aaatataaat accactgtag taggatatgg acatgtagaa aaagggaatc
     1441 ctgtgcattt agtacaactt ttatgtcaac aaataaatgg ttctgatatt gatccagata
     1501 gtaaatttgg acctgctaca tataatcaag taaaaatact tcaaggaaga cttggtgtaa
     1561 caaaagatgg tgttgttgga aaagccactt ggcaagctgt cggagagcgt tattagaaaa
     1621 gaggttgtcg cactaagaaa tagggcgaca acttttttat atagaatata ttaatcagta
     1681 tcttagagtc tagaaatatt ttacaataaa ttccatataa ctctaccatc tatatcctta
     1741 acattttgac aaccagtcaa aatcatagtc tcacataact cattcctaac cttctctata
     1801 aaaaactcca caccatcaag accaccgcca aaagaagcag taacaaaagg tcttcccatc
     1861 aaaacagcat cagcacccaa tccaagcatc ttaactacat caacaccagt cctaactcca
     1921 ccatcaacaa gtattgttat ctttcccttt acagacttag ctatatctgg aagtacctca
     1981 caagtaccag gagtataatc aagtactctt ccaccatgat ttgaaacaat aatggcacta
     2041 gcaccagact ctacagccat caaagcatca tctacagtca taattccttt tataataaaa
     2101 ggtaatttag tagattcaac caattctcta atctcatcaa ttgtcttagg agaaaaagga
     2161 ttttcttgaa attgattatt aataagtcca caagcatcaa ggtctacacc aactgcaaaa
     2221 gcacctgctt cttcagacaa tctaattttt tctattatct ttgaattatt ccaaggcttt
     2281 ataaatacaa ttccatttcc actattatct ttcaacacat ctaaattatc cagtaaaaaa
     2341 gtatctacat tagtatcgcc aaccatagca taaattccag aattagaaca acctctaaca
     2401 acaggctcta tgtattcttt ttcggaaact ttaccaccca tgttcaatag agtacctgat
     2461 acaggtgcag cgaatatagg agaactcatt tttctaccaa ataattctat ggatgtatct
     2521 ggctttgaaa cattatgtat aactctcata tttatcttta ttttttctag actttttcta
     2581 ttttcaatga aagacgaacc acttccttta ccacccatac caggaacatc tccagcacaa
     2641 gctaatccat tacaaatttt acatacttta catgttccat taaagttctc tctagctgat
     2701 tttagtaagt cattgtaatt catacaaaat ctctcctttt tataatatta aaattgagta
     2761 ttactatatt gaaaatatct taattgtaaa tatagggatg ttattatgat gagctaaact
     2821 agataataca tattagatat tttttattgt tgtagatagt taaattatat catattagat
     2881 agtcaaattt tgaaaaaaaa ctaacttatt taagtataca ttttgaaaaa attaaaaaag
     2941 ctatatatag atttttaaag aaatctatat atagctttta agtttatgtc aatctttttt
     3001 tattaatgaa taaataatat agaatacaat atacaaaaaa gtttcttaaa atgctaagat
     3061 aatccccatt tacaaagcaa catcattata ctgtgtaaat ttgaatcaac aatgttttca
     3121 ccatatttca ccataaaatc ttctaattgt gtgtcaataa atgaataata tccacttata
     3181 ctatcgatgt atgcagtcat taagtgtata cattcatcac ttttatcttt atacatatat
     3241 atctccccct atatacaata tataaattta gatactaaat cgtatcatat taaatagtta
     3301 agtgtgtaac atctatgcag ttatatattt atatccttat attaataact aaagtgagtt
     3361 ttgacaatag attttgggaa actggatgtt caaagtgtaa acttggattt taaaatcatt
     3421 aattaagata gtgtatagga atatatgtat acataactta attataaaga ttataaacaa
     3481 ttacttttat taactttatt taagtggtag aataacttgg agattaataa ttataaggag
     3541 gatataatat gagtttatac aagtttatat atgatgataa ggaatattta ttaaaagagg
     3601 agaactgtag tgctttaata aatgatgaag aaaatccagt tcaaggagta agtatttcaa
     3661 aaataattga tatacttaat gaagcagaag aagttgattt tgatgtagaa tattatcaag
     3721 aagcatgtcc attatgttta gaaggagtaa aagaaaagaa aaaattcttt ccttttttag
     3781 aatatcactt ttatattttc agtaaagatg gagaatatgt aataagcaat atatctgttg
     3841 attataaagg actatctttt aataaactat caagggcaaa taaagttgat aatagttata
     3901 ttgtaagtgt gataatttgt gaaaattgtc aggattatat agttcagata gaaaattgta
     3961 tagtttagtg taaagtatgt cttaatttaa gagaaatttc taaattgaga catcaatttt
     4021 tttcaaaaat agtatggaaa attactaaat ttatgagata atgtaagtcg gaggtgagtt
     4081 acaatatata gtaaatattt ggaagagata gatgatacaa ttttagattc aatgtttaaa
     4141 ctaaccttga atgaaagatt attgactaat aacgattttg ttgaaaataa aattggtgta
     4201 gtaaagaatt taattgattg ctataaaact gaacaaaata gtttaatgtt attaaaatca
     4261 aattttttgc tagctttatt atatggtatt caagggttta gtgaaaaaat gaaagagtat
     4321 attttgattt cctgtaaata tattgataaa tgcttaccca aagattataa atttttagca
     4381 cgattttatt cacaaatagc tattttaagt ttaaaaaatg aagatgtaaa taaggcaaat
     4441 ttatatatgg ataaatttaa tcttatttgt gatgaaaata attttctaat tgaagaaatt
     4501 atttttaaat cacaacttat ttacattaag gcaagtaagt gtatagaatc ttctgtaata
     4561 attaaagaaa tagaaacatt gtatataaag ataaaagaaa ttaacgattt taactgtaaa
     4621 aaaatatatt tttttattat tggtaaaata tattttttgt ttttggatga tgctctaatt
     4681 gctaaagtaa attttttaaa agcaagaaaa tatgcagatt tactaaagga tatgcaagta
     4741 tcttcactat gtaatataaa gataggtgag tgtgaatgtt catttgaaaa ttatgaatgt
     4801 tccatagaat atttaaatga agtaataagc aataaaaagt atagaaatgt aaatataatt
     4861 caaaaatata gagcatcaaa caacataact aaaatattaa taaagactag aaattatcct
     4921 gtagcaataa ataatttagc aaaatctgaa gtatatctag aaaaaattaa aaatcagagc
     4981 ttaaaagaag tagagaagtt tgatttattt ataagtttag ctatgtatta tgcaaaaagt
     5041 aatgaaaaat cttttgaaca atcaatatgc tatttagata gagctaaaaa catcttaaat
     5101 atgataaaaa atatagaaga atatacaata tatgatttag aaataattta tcatcaggca
     5161 aagatatatt atatctttgg atattatgac aatgccttaa acacaaataa aaaactcctt
     5221 gaaaaatcta aaaatgctaa aaattataat tatgtgaagt tagcatacaa gaatatatat
     5281 ttgtgttttg aaaagattca aaattatgaa atgagtatga aatactttaa aatgtattat
     5341 gagttaaaaa tgatgtatat aaaagctcga aatagaaatt atatagattc cttaaattat
     5401 agacatgagt atatagaaac agaaataaat aacatgagaa aagtaaaact tgatttagat
     5461 aaaaaaaaat atgttgacca tttgacaagt gcatataata gaactttttt gaacgatttt
     5521 ttagaaaaac aagaagttag tgaatattgc acagcattta tgatagatgt agattacttt
     5581 aaagaataca atgatactta tggacactat aatggagata ttgctcttaa aaatataact
     5641 ataataataa aaaaatatct aaaaaaagac atgtttatga taaggtatgg tggagaagaa
     5701 tttctgatat tatctgtatg taaagattat aaaaaaagca aaatatttgg aagaaaatta
     5761 tgtaggattg taaagaaatt tttaaacaat aatttgacag ttagtattgg cattgataca
     5821 tgtaaaaata gtagtataga tataagagaa ataattgaaa atgctgataa agctttatat
     5881 aaagcaaagg aaagtggtag aaatagatgt ttgcattatc atgattttaa gaatttttaa
     5941 ctgttgaaat aggattggtg atttacttga aaaggagttt aatagaagat ttaaagagaa
     6001 atataaacaa gatagaatac gatatgttga ctaaattcta tctaaatttg acagatttaa
     6061 actacatctt ggattatcac aaagaccttt taaatacaaa actagaagat aatagtagag
     6121 tctatacttt atatttaatt agccttataa actatataaa aaaagataga gaagcatcat
     6181 acaactattt gatagagtca ttaaagtata ttaaaaaatt gtcattaaaa gaagattcag
     6241 atataatatc taaaatttat atttatctta ctttatcagc tatttctata agaaaatatg
     6301 ataaggcaaa taatttttat ttctcagcaa aaaaagttat aagacaaaat catttaaatg
     6361 agttgctagt cttgttaaat atgagtttat gtgtagaaat aagtgcttta tatcagacca
     6421 aaacggatgt aatagcttta gtagaagaag tgtctttttt taaaacttta aaaaataaag
     6481 ctcttgtaag tagggcaaat tatatatttg ggagagttta tctatattta tttaataact
     6541 ttataaaaag tatggagtac ctaattgaat gtctaaaact ggctcaagaa aataatttat
     6601 attctataga ggctttttgt aaatgtataa taagtttgtg ttatttagag agtggaaata
     6661 atgtagaagc tataaaatac ataaataatg tattagatgt tgcgcacagt aagaactcaa
     6721 tatcagtaac tgaaagagtg tctataaaga tagaccttat atgggcatat ttaaaagaag
     6781 atatgaataa agaagcagaa gttattttat taaagactat aaaattaatg aaagttgttg
     6841 aaggtgtata taaggattat ttatattcat ttatattttt atattcagct gaaattgagt
     6901 taaggaaaga taattgtagt tttgaaaagg taaataccta tttaatttta tcaaaaaata
     6961 tatataaaag ttttggcgat gtttttatat ataattctaa actagattac atatataagt
     7021 tgtatggaga tttatatata aaatttggta atatagatga aggtataagt tgttatataa
     7081 aaggtttaaa tttagttaaa aagagttcat tgaatcttag aaaaatatct atattttatg
     7141 ggctaatagc aaatggatat gagctaaaaa aagattttaa actggcaata gaatattata
     7201 aaagtatgga ttggtattct gataggtggg aaaaaaataa ttcctataaa acatctcaag
     7261 caattcacaa acaatatgaa ctcagacaaa aacaagagag tttaagatta ctagtggatg
     7321 ataataagaa attagaaaat gatattttaa aagatggact tactaaacta tataatagaa
     7381 gatatttgga acagcagttg gatttattta atggaaccag agacaaaaat atggttgcaa
     7441 tactagtcga tatagattgt tttaaaagtt ataatgataa ttatggtcat ctagcaggag
     7501 ataaagttat agttaaggta actgatatta taagaagtgt atttatagat attaccgaac
     7561 atataatcag atatggggga gaagaaattt taatactagt agattctata gaatcaatcg
     7621 gtgttgaaga gattgataat atagaattat atattaaaag agttttcaaa cttcttgaat
     7681 ttgagggtat tgaacatata tattctaaaa taaaaaacta tataactata agtgctggag
     7741 taagtataaa aagatgtcgt agtagaaaag atgttgaaat attaattgaa gatgcagata
     7801 agaacctata taaatcaaaa aaagcaggta gaaatcaata cattataatt taaaattgag
     7861 gtgattttgt tgcgaaaaaa aataggaatt atagcctctg atattgaatt aaaagaaaga
     7921 atagaagaat tatatagaga agatgtagaa aatggaacta taataatcga catacttaac
     7981 ctagacttaa tggaaaatca aggtcgtata cttgttgaaa aaggtgccca agctataatt
     8041 ggtagaggtg gaggttatag tttagtaata gatacagtaa atgtacctgt tataccaatg
     8101 aatatgaagt caactgacct tttaagagca atagaaatag caaagaaata ttctaaaaaa
     8161 gtagtattga ttcttggaga caatgaagtt tcttttgact atgttggctg gagaaatgtc
     8221 ataagtactg aaataactga agaatggttt gaatctaagt atgagataag aagtaaagtt
     8281 gtaaaatata tagaccaaaa agatgaagtt gtaatcgtag gaggaggttt ggcatgttct
     8341 tttgcaaggc aatatggaat tgatagtgta tttgcaactg ctagtgatga atctataaga
     8401 gaagctgtag aatattgtaa aaaattattg gatacattag gtgaagaaaa gtttaataat
     8461 gaagtgttac gaaatatact tgatggtata aaagatggag taattgctat agatagtaat
     8521 ggtagtataa ttttgtacaa tgaaagtgca aaaaacatgc ttaaagtaga gagaaaatgt
     8581 gcacttaata aatacatctt agatgtattt cctaaaatgg agtggatgtt agattgttta
     8641 catgaaaaag aggatgtaga agatagaaag ataagaaata taaataatct aattgttaat
     8701 actaggacaa cattaataaa agttgataat agtacttatg gagttttagg aataatacaa
     8761 gatataacca agctacaaaa cttagaaaga aaaataagat ttgacttgaa tcaaaaaggt
     8821 ctctatgcta gatatacttt tgatgatttt ttatttaaag ataaattaac tagagaattt
     8881 attgaagaag ctaaaaaaat tggaaaaagt gattatacaa ctttacttta tggagaaagt
     8941 gggtctggta aagaaattat agctcatagt atacacaata taagtaaaag aaaagatagg
     9001 ccttttgttg ctataaattg tgctactata gcagaaaatt tacttgaaag tgaacttttt
     9061 ggctatgaag aaggtgcatt tactggtgct agaaagggtg gaaaaagagg actatttgaa
     9121 cttgctcatg gagggactct ttttttagat gaaataaata gtctatcctt taatattcag
     9181 acaaaactat taagagtaat agaggaacgt caaataatga gaattggttc tgattatata
     9241 atacctctgg atataaggat tatagcagca acgaacgaat ctttaaccga aaaaatagtt
     9301 atgggtacat ttagagcaga cttattttat agattgagta gtttagagat aaatatacct
     9361 cctttaagag acagaagaga agatataata cctttgttta acaattttgt aaatgaagta
     9421 ttaaaggatg atggtctcaa tggaataaac agtatagatg aaaattttgt tttgactaaa
     9481 gatgaaatgg ataagctata taattatagc tggcctggaa atgtaagaga gttaaagaca
     9541 atagctcaaa aatatgttgt gacaggtaaa attaaattga gacaagatag agattttaaa
     9601 actaaaaaat tacttcctaa ctctgaagta gacaaattta attctgaaac gacagcaagt
     9661 gtagaagtcc aagatgaatc tataaatata agtaagataa atgatggtaa gataagtata
     9721 gatataaaag aagtaaataa gtatgttgaa gaaaaaataa taagtatgct ttttgctcag
     9781 ggactttcta aaaatgaagt tgcacaagtt cttggaataa gcaggacatc tttatggaag
     9841 aaatacaata aaaatattta aatataatta catataaatt attttgaagt aatgttaatt
     9901 ttttaaaaca agtgttaaga aaaattaaca ttactttttt gtaaattttt atttttttag
     9961 acaggcatat atatttataa gaaaaatagt taaaacactt aatttaataa tgaaaaaaat
    10021 aataattcta aacaaaatca attttacata aagtgggatt tattggcata gtaattgctt
    10081 aatatataat gtaagagtaa atacaatttt aggggtgatg gtatgagtca aagtatggtt
    10141 ggtaagcatg caatgtggcc aaaagaaaat gatgttatat ttagcatatc tggtagagct
    10201 caagcagcag agaaggcttt tggtatggat aatgtaataa atgcaacaat aggcgctcta
    10261 atggatgatt caggaaaatt aataacaatg aaaactgtat atgaagaata taaagcatta
    10321 gataactgtg agataggtgc atatgcagct cttgaaggtc aaccagacta cttagaagca
    10381 gttaaaaaag tatttttcag agattactta ccagaaggac atataagagt attagcttca
    10441 ccaggaggaa gtggtgcaat aaaattagct gtatggaact atactaatga aggagatgag
    10501 gtattaacat ctgattggtt ttggagtcca tatgtaagta tagctgaaga ggctaataga
    10561 aaagtagtta attaccaact ttttgatgaa aatagaaggt ttaattttga gtcgttcaaa
    10621 gaaaaatttg taaatatagc agaaaaacaa ggtagagtct ttacaattat aaacacacca
    10681 gcgcataatc caactggata tagtgttgca gatgatgagt gggataaaat attagactta
    10741 tcaaaagaag tcgcaaaaga taaagacaaa aagataatat ttttcgtaga ttcagcatat
    10801 attgactttg caggtgatga tgatgtatgt agaaagtttt ttaagaaatt ttctaatttg
    10861 cctgaaaacg ttctcgtatt ggttggattc agtatgtcaa aaggatttac agcttatggt
    10921 atgagaatgg gggctataat ttgtatatct tcaagtgaag atgtagctga agagttccat
    10981 tactcatgtg ttcattcatg tagagcaaat tggtcaaatt gtaatagaag tgccatggca
    11041 gtattatcta acattgtaaa tgaccctaaa aaattcaaag aatatgagga tgaaaaagaa
    11101 atatacaaaa acatgcttac tagaagagca gatgtatttg tagaagaagc tgaaagagta
    11161 ggattagaaa ttcttccata tatagcaggt ttctttgtaa gtataccatg tgataatcca
    11221 aaagaagtat gtgaagaatt gactaaacat aatttatttg cagttccatt aaaaatgggc
    11281 ttaagatttg cagtttgtgc agtttctgaa gataaatgta agaaagcacc aagtattata
    11341 aaagaagcac ttgaaagttt agaagtaaaa ataaataatt aatttcattg aacaaattgg
    11401 gtggactatg aatttaataa ttgtagatgg agggaatttt atatgaaaca attgatgaca
    11461 ggtaatgaag caattgcacg tggagcctat gaggctggtg ttaaatatgc atctgcatat
    11521 ccagggactc caagtacaga aatattagaa aatatagcaa catataaaga tgcaatagtt
    11581 gcagagtggg ctccaaacga aaaggtagca cttgaggctg ctataggggg ttcaattgca
    11641 ggagcaagaa caatggcttc tatgaaacat gttggtttga atgttgcagc tgatccaata
    11701 tttacttatg catatacagg ggtaaatggt ggtatggtac ttattacagc agatgagccg
    11761 ggaatgcatt catctcaaaa tgagcaagat aataggatgt atgcaaaatt tgcaaagata
    11821 cctttatttg aaccatcaac tagtcaagaa gctaaagata tgataaaaga agcttttgaa
    11881 gttagtgaaa aatacgatac accagtttta tatagagtta caacaagact ttgtcattca
    11941 aaaggattag tggagtgcta tgatagagaa gaagttgaaa ttaaagagta tgttaaaaat
    12001 gctaagaaaa tggtaactgt tccagcaaat gctcaaataa gaaggggcgt tgttgaagaa
    12061 agaatggaaa tccttaaaaa attctcaaat gaaactgatt taaactacta tgaaatgaat
    12121 gatacaaaaa taggtgttat agcttctggt atgtgttgta attttgccaa agaagttttt
    12181 ggaaaaaatg catcatatat gaaactagga tttacaaatc cattacctta tgaaaaaata
    12241 aaagaatttg cagaaaaagt tgataaaata tatgttatag aagaaaatga cccatttata
    12301 gaagaacaaa taaaagcata tggaatagat tgtattggaa aagatgtaat accaccatat
    12361 ggagaaatga caccagatgt tttaagaaaa gctatatttg gaaaaacaaa tgatactata
    12421 gaatacaaat ctgaattagt aacaccaaga ccaccaagtt tctgtgcagg atgtccacat
    12481 agaggattct tctatgaact tggaaagaga aaaaatttaa tagttggtgg agatataggt
    12541 tgttatactc ttggatttgc tcctccatat aatggaatag attatgttgt ttgtatgggt
    12601 tctgcttttg gaactgcaca tggagctcaa aaagttctta atatgaagga tgataatgaa
    12661 aaaagattgg taggtgtact tggagactca acattcttcc atacaggaat aaatggactt
    12721 ttagatgttg tttacaatag aggtaattct atttcggtta tattagataa tagaataact
    12781 ggaatgacag gtcaccaaga aaacccaggt tcagggtata ctttacaagg tgctaaaact
    12841 aaagaagttg atatagaagg attagttaaa gcttgtggaa tagaacatgt aagagttata
    12901 aaccctaaca atttaaaaga agtaaatgaa gcactagatt gggctttagc tatagaagat
    12961 gaaccatcag taataataac aagatggcca tgtgtactta aaaaattctc taaagaagat
    13021 atagaagaat ttaataatcc atttaagact aagtgtaaag ttgaccatga taaatgtata
    13081 ggatgtaaat tatgtttgaa aactggatgt ccagctctat catttgataa agagaataag
    13141 ttatcaaata tagatagaaa tcagtgtgta ggatgtggag tatgtgcaca agtttgtcca
    13201 aaacaagcaa tagttaagga ggaaaaataa tatgactaag agtatacttt tggtaggtgt
    13261 aggaggtcaa ggaactatac ttgctagtaa attattaact atgggattga tggaagctgg
    13321 atatgatgta aagatgagtg aaatacatgg aatgagtcaa agaggaggtt cagtttcctc
    13381 acaagttaga tatggagatt gtgttcattc tccagttata gaaattggtg gagcagatat
    13441 attagtatct tttgaaaaaa tggaagcttt aagatggttc aattatttaa aaccagaagg
    13501 aaaagcttta gtaaataatc ataggataga ttctatgact gttttaatcg gaggagctga
    13561 atatcaagaa agtgatatag atgcagagtt aagtagatta aatgcaaaag ttataaatgc
    13621 agctgataaa gctgaagaac taggaaattc aaaaataatg aatgttattt tattaggatg
    13681 tctagttaaa tctatgcaat tagagtctat tgattgggaa aaaattataa gtgaaaatgt
    13741 taaaccaaaa tttgttgaat taaacataaa agctttccat gaaggtatgg aaatggttag
    13801 caaatagttt ataatagata taaatagact atagaaatta aggtgaaagg aagaaaatta
    13861 tgacttacag aatattagcc ataaatccag gttctacttc tacaaaaata gcagtatatg
    13921 atggagaaga acaaattctt gtgaagacga tagaccatcc agctgaagag attgcaaaat
    13981 ataatactat acaagaccag tttgaaatgc gtaaggaagc agttttgaat attcttaaag
    14041 aaaatagtat agacttaaaa tctcttagtg caatagtagg aagaggtgga gttttaccac
    14101 cagtaaaatc aggagcatat ctagtaaatg aagaaatgat tgatgtacta agacatagac
    14161 cagtacttga acacgcttcc aatttaggtg ctgttgtggc acatgcaata tcagaacctc
    14221 ttggaatcaa ctcatatatt tatgattctg ttgcagtaga tgagcttata gatgtagcga
    14281 gaatatctgg actttgtgga atggatagat caagtgcagg gcatgcatta aatactagag
    14341 caatggcttt aaaatatgct aaggataaag gaaaagatta taagagctta aacttaatag
    14401 tagctcacat tggtggagga gtaagtattt atcttcatga aaaaggaaga atggttgata
    14461 tgctatctga tgatgaagga ccattttctc cagaaaggtc aggaagagta cctgctacaa
    14521 aattagtggc tgcctgttat tcaggtcaat attcagaaag agaaatgact aaaaagataa
    14581 gaggtaaagg tggtatagtt tcatacctaa atactgtaga tgctagagaa gttgaaaaaa
    14641 tgatagcaga aggaaatgaa gaagcaaaaa ttatttatga agcaatggct tatcagttag
    14701 caaaaggtat tggagagtta gcaactgtag tagatggaaa ggtagatgct ataattataa
    14761 caggtggaat tgcatattct gaaatgttta cttcaatggt taaaaagaaa gttgagttta
    14821 tagcaccagt agaaattatg gcaggagaaa atgagttgga atcacttgct tttggaactt
    14881 taagagtact aaatggagaa gaagaagcta gaatttatag tgaaaattaa taagaaaaat
    14941 taaaaatgtt tatatactaa aaaagtggta tatccgtttt ttatacacga aagcttagag
    15001 tataggtgaa aatgtataaa atttggattg ccacttttta taattaaaat tttatcttaa
    15061 tctaataaat aattctgtaa aatttcgtat ttatatatat attttattgg tatttattgt
    15121 tattattatt atgaggagtg atttttgatg ggaaaaataa gtttaaaaag tatgataaga
    15181 ttattttcag gtttttttat atttgcaatt tcttctgtgc ttatgattaa tgcacatgta
    15241 ggtcttatgc cttgggatgt acttcaccaa ggtttatcta taaagctagg cataactata
    15301 ggtcaggctt ctataatggt aggagttgta attgttatat tggatgcagt ttttggtgaa
    15361 aatataggat ggggtacttt actcaacatg acatttatag gaatttttat tgatttagta
    15421 atattttctg gagtgatacc tcatgcaagc aacacttata taggtgtgtt tatggttgtt
    15481 ataggaatta ttttggcagc tatagcaagt tttctatact taggtgtttg tttaggaagt
    15541 ggaccgagag atgggcttat gattgctctt cagaaaaaaa ctaataaatc tgtacgtctt
    15601 gttagaacaa ttcttgaaat actagcatta gttgttgggt ggttattagg aggttctgta
    15661 ggtattggta cgcttgtaag tgctttaggt ttaggatatg tattacaaat agtgtttaga
    15721 atatttaaat ttgacacaaa gttgctgaaa catagattta ttatagatga tataagagaa
    15781 tggaaggaaa agagaagcaa tgaacataaa tgtaaatcaa gtattgtaat aaaaaatgag
    15841 cagaattaat tatgctaaaa tatatttgta gtttatctaa tttatagtaa agtcaggaga
    15901 ttttgaaatg gaattatggg atttatataa tgctgatgga attaaaacag gaaatgttat
    15961 aaaaagaggt aattctattg aagaaggtta ttaccatttg gcagttgaag tgtggatttt
    16021 aaatagtaac tctcaaattt taattcagaa aaggtctaaa agtaaaaaga cattgccaaa
    16081 tatgtgggga atgacaactg gatgtattgt ttctggggaa gatagtttag aaggagcaat
    16141 tcgcgaagca aaggaagaaa tagggataga tataactaaa gatgagatga aagtttttag
    16201 aagtatgatt catgaagata ctctttggga tgtatactta gttaaaaaag aatatgatat
    16261 atccaaagct attttacagg aggaagaagt aagtgatata aagtgggtaa gcacagatga
    16321 aataagacaa ttactaaaag agggtttgtt ttttgaatat ccagagatgt acgaattgct
    16381 atatgagata gataataata aattaaatat atgatgtaga gcagatttca gtataagtaa
    16441 gattatttaa aatgtatctt ctctaaattt taatattgtt ttatgaaaat taataagtta
    16501 tttttagtat ttttctactt gttttttgag attaaaataa aaagggcagt cattataaag
    16561 tcataaaaat ttggggagga tttaaactat ggaagataac aaaggattgg cacgtgatag
    16621 ttcaaaatat aaaattgttt ctatatctgt cgtagtaatt attctaatta cattttggta
    16681 tatgttaaat atggtgcttt taacatttat tatgactttt attttctata acttgcttgt
    16741 agccacaaga aaacgaatta agaagttttc ttctttaaat attccagatt cattgataat
    16801 tatagtgtta tatgcactat ttgctatatt gttagtattg attagctatg cagttgtacc
    16861 aataattata gttcaactta cagagctgag tagggttttt agtgattttg atgtaaatca
    16921 atttgctcaa tctttaggac caaaattgta tcctattgtc tcaaagctgg attttaataa
    16981 atatatttca caagcagggc tacttatagc atcaacagca acaaaagttg gaagttttgg
    17041 agtaaatata ttattggcat ttttactgag tttactcctt ttacttgaaa aaaatgaaat
    17101 taaaaatttt ggagataaac tttctgatag taagatttct tttatttata attcgcttgt
    17161 attttttgga aagagctttg ttaaaaattt tggtgaagta atgaaagtac aagttatgat
    17221 tgcctttata aattctgttg tttctatgat tttcttaggt ttcatgggat tccctcaaat
    17281 atgggcttta ggatttatga tttttgtgtt gggtcttatt ccagtagcag gagttatagt
    17341 gtctttgatt ccattaactg taatagcatt taatacagga ggaataacaa aggtttttgg
    17401 agttttactt atgatttgta tagtgcatgc agtagaaaca tacatattaa accctaagtt
    17461 gatgtcgaat agaactaaac ttccagtttg ttttgttttt attattttac ttgtaggaga
    17521 acattattta ggtgtatggg gattactaat tggagttcca atatttatgt tccttatgga
    17581 tattctgggt gttaaattta cttctaggta atatattaaa ataataattt atttttatat
    17641 ataaattatg aattgtttta aaaaagaacc tataaataga catcattaat aaagacatct
    17701 atttgatagg ttcaattttg ctttgtataa atactataga attaaataaa aagaaattta
    17761 tttcatttca aaagtttcac aacttgcatt tctttcattt atatgaactt tttcagcttt
    17821 gcaaagttca tttttattgt atttacattt tacagcttca caatgaatgt tatctgtatc
    17881 aacttggtta gtttcattag aacgactagt tataccagat gtatatttgt cttcaaaaga
    17941 agcacaataa gtattttctg tagtagttgc ttgcattcca tctactttta tactaccagc
    18001 ataacaatat ccactagtat tataagcaca attagtagca gcacaattta aattattatt
    18061 tttcataaat aacctcctaa gttttattta aacttattat gtgaagttat ctaaatttta
    18121 tacacttatt tacaattaca tttatcacat ttaacaacta catctttgga agtttccctt
    18181 cctaaatttt tattaacatg atacacatca acattttcag ttgatccaca tatagaacat
    18241 ttaaaattgt tttctagtag ggttctttct ttaaataact tccaatacat agaattgatg
    18301 taatcattat aatttacctt gaggcatgtc tcaagaaagt ttatgtatag attgtctaaa
    18361 tctcctgtat ttaattctgt atattctggt tcaaaatatt cacaacatcc tctaaaatca
    18421 ggctttgtat ctgaaataac acatctaata tcatatctat agtctaggct aggcccctta
    18481 aatgaaagat gtgtgcaaaa agaacaagat gtttctggta gatttttttc aagttcatgt
    18541 aatttattat tcataagtat tcctccaaaa aatttatttt aagtatataa atgaatagat
    18601 tattttaaaa cttttattta tttatattag agatttagga taaatattcc aataaggagt
    18661 aaatacttaa gtatttatta ctgtattaaa taaaaaagag gcaaatctaa cttgagattg
    18721 cctacttttt tatcattttt taataaaatt tattctaagt attattttat ttagatttag
    18781 tcagccaatt tagtatccgc tttgcttttt aactttctac cagagcgtac agttaaaata
    18841 gcatataata cagtaaatat ggcagctaaa acatatgata tatttatgtt taagttaaat
    18901 ccaatttgag cattaaatat ataactgaat ataacaaaag aataaaacat accaggaatt
    18961 aagcttataa tataattctt ttcttttatt ataaggtaaa ctgtaatcat tgcaaatgca
    19021 aatatggcaa tagtttgatt actccaagag aagtatctcc acaatatatt aaatccagaa
    19081 gcactaagct ttgcaaatat aagaatggct atgacaggaa taaatataca gattgacaat
    19141 ataacacgat gtttcttttc cttttggtca taatgaaagt aatctgcaag cattaatctt
    19201 aaagatctta aagctgtatc tccagaagtt attggtaaaa caattacacc tattatagct
    19261 atgataccac caatagaacc aagtaaatct cttgcaacca aacctattac atcaggagaa
    19321 cctactaatt ccttaggtat tcctttattg taaataccca tagcagctgc agcccaaatc
    19381 attgcaatta aaccttctaa aatcatcata ttataaaaag tagtttttcc ttctttttca
    19441 tttgaaactg atcttgctat aagagtagct tgagttgagt ggaatccaga aacgatacca
    19501 caagcaactg taacaaagaa tgtaggaata agaggaatac catcaggatg aatacctttc
    19561 caatttgcaa gacttaaatt agctaaatca taaccttgtg taaagattcc aattccaaca
    19621 ccagcagcag ataaaagtag aatagctcca aatattggat atacctttcc tatgatttta
    19681 tcaattggaa agagtgttgc acacaagtaa tatagaaaga taagtccata aactatccag
    19741 acaactggat tatttatagt cgatttcata tttaaaattt gagtaacaac taaatcccca
    19801 ggtgtataaa taaaaactgc accaactaaa agcattaaaa gacaaaggaa tatattatag
    19861 acttgaaata ctttattacc taaaaatcta ctgataagag aaggcatttg tgctcctttt
    19921 tctcttatac taatcattcc actcatatag tcatgcaaag ctcctccaaa tacacagcca
    19981 ataggtatta gtatgaatgc tattggacca aacaatatac cttggatagg tcctagaatt
    20041 ggtcctgtac cagctatatt aagcaactca attagactat ttttccattt tttcattggg
    20101 acatagtcta ctccatcact ttgagcaagt gcaggtgttt ttctgtcatc tgggccaaat
    20161 actttttcac aataagcacc gtataaagca cctcctatta gtaatatagc caagccgatt
    20221 aagaatgttg tcataatatc accccattta taaaattttt tggtctttgt atacatatta
    20281 tctaaatgtg tagatttata ttgaatttaa ttctggacaa cctacaaatt ctataaagta
    20341 ttacaactaa tataatttca gaaataaata catatacata accatgtact tagtatacag
    20401 gtatttggta atattttcaa tgttatagca ataaagaata taaacaaaat tctgataata
    20461 ttttacaaaa aaattaaaaa atataaaaat tagtttaaat agatggagaa aaaaggaaaa
    20521 tcaattgaga atataaatgt ctatatctta acaatattaa gaggtttaaa attcttgaag
    20581 aaagaatagt caagtgatat aatgggttta caggtgtata tacactagta catatagaaa
    20641 gcggtgcaat tatggataaa gatttaacat atcaaatcaa agagctaaag aaagaaaaaa
    20701 atgcaataat acttgcccat ttttatcaac caccagaaat acaagaatta gcagatgcag
    20761 ttggtgactc atattattta agtgagattg ctagagattg taaagaagaa gtggttgtat
    20821 tttgtggagt aaggtttatg ggagaaagtg ctaagctatt atcacctgaa aaaactgtat
    20881 tgatgccagt aagtaacgct gggtgtgcta tggcagatat ggtagatgaa gaaggcgtga
    20941 taaaattaaa acaacagtat ccaaatgctt tggttgtatg ttatataaac tctacagcta
    21001 aagttaaggc acattgtgat gtttctgtta catcttctag tgcaattaaa atactagaaa
    21061 acatagataa taaagaaatt atattccttc cagataaaaa tttaggagga tatattgcag
    21121 aacaatttcc agataaaaac tttatatttt gggacggata ttgtaaatac cataacaata
    21181 taagagctga agaaataatt gaattaaaag ataagtataa aaatgcagaa gtattagtac
    21241 atccagaatg taaaaaagaa ataagagatt taggagatta tgtaggtagt acatccggaa
    21301 taataaaata tgctactaat agtaaaaaca aagactttat aatagcaact gaagaaggta
    21361 tattacatga attaaagaaa aataatccta ataaaaactt ttatattcct ggtggaaaaa
    21421 tactatgtac agatatgaaa aagacaacat tagaaaattt atatagtaca cttaaaaata
    21481 tggaaaatga agttattgta gaagatgaaa tcatggaaaa ggctttaaat tcactgctta
    21541 acatgcacaa attagcagaa ggatagaggg atagacttat gaatttagaa caagatgttt
    21601 taatagttgg aagtggagtt tcaggattat actgtgcatt aaatttagat aaaagtttaa
    21661 atgtgctagt agtatcgaaa tcaacaattg aaaacaacaa tacatatctt gcacaaggtg
    21721 gaatttcaac tgctagaaat attgatgata tagaatcctt tgtagaagat acaatgaagg
    21781 ctgggcagta taaaaataga gttgaagcag ttcaagtact tgcagatgaa tctatagaaa
    21841 atgtagggca gatagtagaa tatggtatgc ctttagacaa agaaaatgga gagatagatt
    21901 acacaagaga aggagcacat agtgtaaatc gaatagttca tagtaaggat aatactggag
    21961 aagttgtttt taaaacttta ttaaaagaag ctaaaactag agaaaatata actcttatag
    22021 aagatgcata tcttttagat atattaaaag atggcaataa atgtatagga gctagaattt
    22081 ttaaatctaa aaaagagatt catgtttttt caaaaatagt agttttagct acaggtggaa
    22141 ttggtgggct ttttaaaaat tctactaatc aaagacatct tacaggtgat ggaattgcaa
    22201 tagctttaag aaataatata aaaatagaaa atttagaata tattcaaata catcctacag
    22261 ccttttatga agaaaataat gaaggtagga gaatgcttat atctgaatca ttaagaggag
    22321 agggagctaa acttttaaat aaaaataaag aaagatttgt ggatgaactt cttcctagag
    22381 atgtagtttc aaaagctatt tttgagcaaa tggaaaaaga taaattacct tatgtgtatt
    22441 tagatgcaac acatttagat agtgaatatt taattaatag attttctttt atttataatg
    22501 aatgtttagc tagaggaaca gatataacga aagaatgtat aaaggtatct cctgcacaac
    22561 attattttat gggcgggata catgtagatt tggattcaaa aacatctatg gaaaatttat
    22621 atgcagttgg tgaaattagt tgtacaggtg ttcatggagc caatagattg gcaagtaatt
    22681 cattacttga agggcttgta ttttcaaaaa gagcagctaa aaatataaac tcagttatag
    22741 ataatgtaaa agttaagttt atagatgtac cagatatgga tatagatata gaacaggtaa
    22801 aaaaagaaaa taaaatatta gtcataaaag aaattgaaag aacaagtgag gattttggcg
    22861 atgaattatt tgattattga tagaatgata caggatgctt tgatagaaga tgttccaagt
    22921 gaagatataa caactaattc tattgtggat gaaaattcta aatctactgt agatttaatt
    22981 tgcaaacaag atggtataat agctggctta ggtgtattta agagagtttt tgagatactt
    23041 ggtgatgtag atgtaaaatt atataaaaat gatggtgata gagtaaagaa tcgtgaaaag
    23101 atagctgtat taacaggtag tactagaaac ttacttgtag gagagcgtgt agcattgaat
    23161 tatttacaga gaatgagtgg tatagctact attacaagta ggtatgttga aaagcttgaa
    23221 ggtacaaata caaaattatt agatagtaga aaaacgatac ctaatcttag aatattagat
    23281 aagtattctg ttaaagtagg tggaggatgc aatcatagat ttaatttatc tgatggaata
    23341 ttgcttaaag acaaccatat tggggctgct ggtagtgtta agaaggcagt tgaattagct
    23401 agaaaaaata cttcttttgt tagaaagata gaagtggaag tggaaacttt agacatggta
    23461 aaagaagcaa tagaagctaa tgcagatata ataatgttgg ataatatgag tttggaaatg
    23521 gcaaaacaag cagtagatat tataaatgga agagccattg tagaattttc cggaaatgta
    23581 aatttagata ctatagaaga tataggaaaa attggtgtgg atgttgtttc tgtaggagcg
    23641 cttacacatt ctgttaaagc tctcgatata agtatgaaga atttgagaaa tatataagtt
    23701 ttatattgaa ataaacttaa ttaaaataga ctccttaaat ttgttttata aatttaagga
    23761 gtctattttt tattcattaa aattaataaa aatataaaaa aatcaatcaa taatttaata
    23821 aaaatagata aatactatat acacttttaa taatatagct aatatctatt ttacagataa
    23881 ataagaataa acttataata taattgtatc tgtaaaattt tgtaaaatat aaaaagtata
    23941 gggggaataa aaatgaaagt agcttatatt tttagtagta ctaatacaca taaaattcta
    24001 gacaaaatga taataccaca acttgaacaa ggaactcatg gagtagacgt tctagggatg
    24061 tttttcttta tggataatac cttattttta tctaaaggta atccagtagg cgagagacta
    24121 agtaaattac atgaaaaaac tgatatgata ataatggctt gtgaccaatg tgctatagaa
    24181 agaggtatag agaataattt ggttgatgga gctacaattg gatgtttccc aaacctatat
    24241 gcttgtttag gcagtgcagg tgtggaccaa gttataacgt tataaattag tataggatac
    24301 aatccataga aaaatttatt ttctgtggat tattttaagt aaaagataaa ttgtttaaag
    24361 taggggatga acaaatgtct aaaattttag ttgaatttat aaacacttga ccatcttgtg
    24421 atgaacatgg agtgtttata aaaaaaatgt cagaaaagta tgctgataaa ttggaaataa
    24481 aattatatca agcagggaaa gatttttcgt atataaaaaa atatgggatt ataacaaaag
    24541 gaactttaat aataaatcag aaaaagaaat atgatagatt aaataaagat acaattgaaa
    24601 gggcaatagt agaagctata aacaataatt aaaagtttaa aaattaggag tatcaaaaat
    24661 gaatatatta gaaattgttt taaatggagt taaagatact atagtagctt ctatagttac
    24721 actgaatgga gcatcaggat ggcttatgat aagttttata ttggcaggtg tacttcacaa
    24781 tgttttaact ccaagaaggt ttcaaaaaca acttggaaat aagaaatttt cttctttaat
    24841 taaaagtact atctcaggaa tgtttttacc aatatgtagt tgtggagtaa taccacttgg
    24901 aataagtatg tattattcag gagcatattt aggaccagta cttgctttta tgacatcaac
    24961 tccaataata aatccaatag ctgtagttct ttgtctaggt cttttaggtc ctaaagttac
    25021 aatcatttat ctaatagctg gttttttagt accattttta gtaggatttt taggaaattg
    25081 ttttggaaaa gaagaattat cactttataa tgattcagag gaagaagtaa tagaattaga
    25141 agaagaaaaa agaagtttta tggaaaatat gattgatggt cttaaatggt cttttggtga
    25201 attagccctt actattagta aatatgtagt aatgggtatg gtttttgcag gatttataac
    25261 aactattttt ccaaatagta taattcaaaa atatttagga aatccaggtt tgctatctct
    25321 tggaagtata gcaatacttg catgtgttat gtatgtatgt gctgtaggac atataccgtt
    25381 tatagcagcc cttgttgcaa gtggagcatc tcctggtata gcgataacat ttctaatagc
    25441 tggagcagct acaaatctac cagaacttat aagcatgttt aaacttattg gaaaaagaac
    25501 tgttattata tattctgtaa ctttgacact gtcctcaata ataattggat atataacaaa
    25561 tttactttta gtgaataata attcagtaaa tttagatgca acaaattctt caatacagtt
    25621 ggcaaataaa ctaatgttaa atatacctga tagttttgag tatgtatgta ctattttaat
    25681 tattttattt gcatttaaat caacaatacc aaagatgaaa gaggtcatag gaaatcatgc
    25741 ttaaaaagat agttataata tcctgtatag tagttgtgtt aatcatttta tcaaaaatag
    25801 ttgatgataa catcaaagaa gatgcttcta ttccaaatgt gaataaagaa acattagaat
    25861 attttagaaa aaattataaa gaagacataa taacttgtgc tgaagaagat ttaaataatg
    25921 atgggaaaaa agatttagta gttatatata aaaaaagcaa taattcaaat gaaatggtag
    25981 tcgtagtttc tgataaaaat agccactata taacaaagcc aataccagca cctatagaga
    26041 atcaaactat aacctttaaa aatattgatg ataaagcacc aatagaagta atagtatcag
    26101 ggtctaaaaa tggtaatgta ggatatgcta tttacagagt agagggtaaa aaatttgttg
    26161 atttatttgg cgaagatatg gacaaatgtt gctaaaaata aaaataatac ataaaggtgg
    26221 gaaatttata tgaaattatc taagaagatt caagcactaa tcttatttgg tgtaatgggt
    26281 acatctatat taacaggatg tagtagtaaa ccaaaagaaa aagaagaagc aaaagctgga
    26341 aaagatgatt atacaataac agtaggttat tataattgtg accatatgac tgcgggacca
    26401 gtagcagaat cggcaggaat atataaagat ttaggtctta atgtaaaaac tgttggaaat
    26461 ggtaaagttc ctgaagctat ggcagcagga aaaatggatg caggttacat aggaactaag
    26521 gggttagttg gagcaatacc taagggttca ccaataacaa tagcagctaa caaccatact
    26581 ggtggttctg aatatttaat agtttcaaaa gatataaaag aacctaaaga tttaataggt
    26641 aaaaaaattg caacagatat gagtgatttc ttatggacaa gtgattatgg tccagaaaca
    26701 ggtctaccaa ctgacccatc aaaatatgaa gttgtaaata tggattcaga caaagataaa
    26761 tatttagctc ttaaaactgg gaaaattcaa gcttttacat cttgtgaccc ttggggttct
    26821 gtagctgaaa atgacggtgc aggaaaaata atagcatcta ctcaatataa agaaaaagct
    26881 aatggcaaag aatataactg ttgttcattc tcattaaaca aaaactttat aaaagaacat
    26941 ccagatttag caaaaaaatt ggttctagca catacaaaag caatagaata tatatataca
    27001 aatccagctg aagcagctaa aatatttgca aaatattata atgtagaaga agaagttgca
    27061 cttagaacta tctataagaa gactgttgga gaaggtagaa ctcttacttg gaaagttaca
    27121 ggtgaagaat ataaaaataa tttacaaatg tataaagatt taaaggcttt agatgatata
    27181 cctaaatatg aagatacgat tgatacaagc ttattggact cttgtggagc tgatgatttt
    27241 gacaaattta taaaagaaaa agttaaccca gagtttcctg aaggtatgag ttatgaagac
    27301 tggaaagcaa aggtattata aataaatgga gaatataaaa aatataataa tactttttat
    27361 cattattttg ggaagtatat ttataatatt ttttaatttt gaagataaag aagaaataaa
    27421 aggaaaatta agaatagggg tatctgatga tacctctggt tttgtaataa attatatggt
    27481 aaatagagac tactttgaaa atataaaagt aagtgatata atggatattt ttacaattaa
    27541 tgattgctga gcaagtacaa tgcagtgggc tctgagttca gaggaaatag atatagcaat
    27601 catatgtaaa gatgctgcaa aacaatatgt aaatgtaaat agtggatttg aaattgttgg
    27661 tactgtagtt caaaattctg atatatttct attaagtgat aataatcctg aaaagatagg
    27721 agtcattcaa aatagagatt accagtcaga attggttaaa aaatactaca agaatgtgga
    27781 agtagctcca ttattaggga ctgcactagc atatggatta gaaagtaatt tagtagatgg
    27841 tgttgtaata gattgcataa agtcaattgg gttagagggt aaaagaaaat ctacaactaa
    27901 attaggagat tatgatacct atgtattagt tgtaagtaaa gagtttaaaa aaagtaaagc
    27961 atatagtgat tttataacac tttacaataa aagtgttgat gctttaaaga ataaagctac
    28021 atttaagaaa gcattaaaag agtacaggaa tataagaaat atagatatgg aggaaataag
    28081 caattggaaa ttaaaatttc taaagataaa agcgatagat taactaagaa aaaaactgat
    28141 ggtagtatgg atattttata taaaataata ctattagtta ttatattttt agtctggcat
    28201 tttgcagcaa aagatatagg gagttctttg ttacttccaa tgccagtaga tgtaataaaa
    28261 ggtttctttt tctgtgtgac agatgcagag acagtcacta atctttttat aacattgcag
    28321 agagtactta aaggttttat gtatgcatta ttgtttgggc ttccaatagg atttattatg
    28381 ggattttcta aaacttttga aagggtttta tctccagtag tagattctgt aaggcaagtt
    28441 ccaataatgg catgggttcc tcttactata gtatggtttg gtataggaga tggtccaaca
    28501 atatttttaa tagctttttc aggggttttc cctataattt taaatactat tcaaggagta
    28561 agagcaatat caaaggatta ttataatgca gctagaagta tgggagcatc gccaattgtg
    28621 atatttacaa atgtaattgt tcccgcatct cttccagata tactaactgg ttcaaggatt
    28681 gctataagta caggatggat gtctgttatc tgagccgagt ttatagctac gagtgccggt
    28741 ctcggttact caatggtaca agctcaaaca ttaatgcata cagatgtatt aattgggctt
    28801 atgatatttg cagctttaat aggatttata atagataggg tattaaagtt tataaataaa
    28861 atcctttgta gatggaggtt tgcagattaa tgaagttaag tgtaagagat ataaataaga
    28921 catttgtaaa taatagagta catacaaagg ttttagaaga tataagtatg gatatagatg
    28981 atggacaatt tgtctgtctt ttaggaccat caggttgtgg aaaaacaact ttacttacta
    29041 taataggtgg gtttcaaaaa tcagaacgtg gagatgtttt tataaatgat aaaagagtta
    29101 aaaaaccggg catagataga gcttttatat ttcaaaatta tgctttattt ccttggaaaa
    29161 ctataagagg taatgttcta tttcctatga aacagcagaa aataccaaaa gaaaaaagag
    29221 aagaaatgct agaagaactt ttggttatgt cagacttgaa aggaaaagaa aaactgtttc
    29281 cgcatcaatt atctggagga atgaaacaaa gagtggccat gataagagct ttagcatgta
    29341 atccagaggt attactaatg gatgaaccat taggagctgt agattttcaa atgagacaaa
    29401 atttacagga agagctagaa cgaatatgga taaagaagaa aataactgca ttaatggtaa
    29461 ctcatgatgt tgatgaagct gtatatatga gtgatagagt tatagtaatg tcgagagata
    29521 aaggaagaat tattgatgat ataaatatag atattccaag acctagaatt agaggaagcc
    29581 aaaaatatga agagtacaaa aacaaattaa ctgatacttt atcaaagtgt tatgaagttt
    29641 aaagaggagt agacatgagt caaatagaaa aatacaatag tttggataat acacaacaga
    29701 aaataagaat ctctattatg gatatgataa tagataaagg ttctagtgtt acacttcaag
    29761 aagtgactga atatgtaagt aaaaagttga atatagaaaa agaatatata gaaaggacac
    29821 tacaatattt tatttatgaa aatataatgg ttgttgatgg gaatagtata aattttatat
    29881 atcctgtatc agcattgcaa acaaatcata aagtaacttt gagagataat agaagtttca
    29941 gcgcaatgtg tgctatagat gctataggaa cctcttgcac attcaaccaa gatataaaaa
    30001 taaactctat ttgtagtgct acaggtaagg aaatagaagt aattataaaa aatgaaaaga
    30061 ttgagtatgt gaataatcca aacttaagag tgcttcatat aaatcttgat aagcatctaa
    30121 attgggctgc tagttgctga aatataatga atttcttctg gacgaagagt gattttgatg
    30181 cttggacaaa cgaagcggga ttatctgatg atgaagatat atattgttta gatattaatg
    30241 aggcaatagt agaatcatat aaaatattta aactcaaaca aaaggtacta tcatagatat
    30301 acatatatga aaaactaaaa atgaccttta gataaaaatg ataggtaata tccatactat
    30361 ataaattagt atagagtagg gatgtgaaaa gtatttaaat ctaaaggtat atttttagtt
    30421 tttttagatg attagatagc cttattaagt atatatttat ttataacctt tgcaactcca
    30481 ttttcattat tagtatcagt aacataatct gcaatttcct ttatactatt acgtgcattc
    30541 cccatagcca caccaagacc tgcatacttt atcatatgtt ggtcattttc ttcatcgcca
    30601 acagcaatag cattttctat aggtatattt agattcgtac ataatgtttt aagagcagtt
    30661 cctttattag tagttttacc taaaaattct agataaaaag gagcagacct tactatagta
    30721 tatttattga ataaattttt aggtagttgc tgtatgattt cttccaatct ttctggttca
    30781 tcaactatca taattttaca gattttaata gattcatcaa ttttatcttt ttgtatatat
    30841 ttgacttcta tattatttaa tttcgcttca tgaagtgtat attttccagg tgcttcattt
    30901 ggtgtaatta aatttgtcat tgtatttatg tgtattttag tcttatattt tttacaaacg
    30961 ttattatata taaaatcaaa atcatctaaa gtcatttcta catcatgtat gattttctca
    31021 cttttaactt cttgaataat tgctccatta aagcatataa cataatcacc ttcatctgtt
    31081 aaatttagtt gatttagaag ttcttttaca ccaggaagag gtctgcctgt tgttataact
    31141 acttttgccc cagccctttt tgcctcctga atggactcaa agacttcagg agtaatcctt
    31201 ttttgtgtat ttaatattgt tccatcaatg tctagtgcga ttaatttgta catatcaacc
    31261 tccaataaat tatatactca aatttcctgt gaaaacgttg acacagctag tgttgaacaa
    31321 agtttcagtg ggaaatatgg tttgttttgt atactattat atcaagagat aagtaattta
    31381 gaaagtttaa taatattaat ttttgataaa aatattactt atgtgagatg agaatgtaaa
    31441 aactttgtaa tcaataattc caaaattctt aattacaaat aatggtatcg tttgcattgt
    31501 taatataaat aaatgtatat tatatagtta gaagtttgaa taaatagact gatggtaatt
    31561 tgttatatta gttagttttg tgttataaaa tattgtaatt ccattaatca tatgttatta
    31621 ttcaacatga ttataatata aagaatattt aatatatata ataatttata aaaaagtatt
    31681 acatagtaca tgtaaatcta tatttataaa tacaataaag taaagttgta aaggagtgct
    31741 ttaaatgaag ttttcagagt ttaagtatga aagacccaat tataatagta tgaaaaaaga
    31801 gtttttgagt tgtgtagaag atataaataa ttctcgtaat tataaagagc aacaaaaaaa
    31861 tatacataag attaatttat taagaaataa aatagaaacg ttgtcaaata tagcttcgat
    31921 aagatatagt acagacacat ttaataaatt ctacaaagaa gaaaaaaatt attgggatga
    31981 atatatgcct ttgtatgaag aattaaattc acatttttat aatgctattg taaattcaaa
    32041 atttaaatat gatttgataa aagagtttgg agaacaattt ttcacaatag tagagtattc
    32101 tttaaaaagt ttttctaaag aaataataag tgaattgcag gaagaaaata aattgtgttc
    32161 agagtatact agacttttag cttcagctga gataatgttt gatgggaata taagaaattt
    32221 atctggtatg gggaaattca tgtattctaa gaatagaaaa acaagagaat tagcaaacaa
    32281 agcatattat aatttctttg aagaaaatga aactaaattt gatgacatat ttgataaatt
    32341 ggttaaactt agagataaga tatctaaaaa actaggtttt gaagattttg tagagcttgg
    32401 gtacgttaga atgatgagaa gtgactacag agaagatatg ataaaaaatg ttagaaaaca
    32461 agtattaaaa tatgttgttc caatggccaa tgagctttat gaaaaacaag ctaaaagaat
    32521 tggtctagaa tatttaagtt acatagatga aggcgtggaa tttttaacag gaaatgcaag
    32581 tttaaaggga gattcaagat acataattca aaatggtaaa aggatgtatt ctgaactgtc
    32641 aaaagaaaca aatgaatttt ttgattttat gttagaaaat gaacttatgg atttagaaac
    32701 gaaaaaagga aaaggagctg gagggtattg tacttatata ccagattata agtcaccatt
    32761 cattttttct aattttaatc aaactgctga tgatatagat gtgttaacac atgaggcagg
    32821 acatgcattt cagttgtata tgtctagatg gattgatatg ccagaaatta attttccaac
    32881 gcttgatagt tgtgaaatcc attcaatgag tatggaattt ataacatggc cgtggatgga
    32941 tttatttttt aaagaagata cagataagta caaattcact cacttatcat cttcaattaa
    33001 atttatacct tatggagtaa ttgttgatga atttcaacac tatatttaca aaaatcctaa
    33061 tgtagataag tcaaaacgaa aagaaatctg gagattttta gagaaaaaat acttacctca
    33121 tagaaaatat ggtgataatt cttttttaga gagaggttgt tggtggttta aacaaggaca
    33181 tatatttaaa aatccgtttt attatataga ttatgtatta gctcaaattt gtgcgttaca
    33241 attttggaaa aaaatgattc aagatagaga tgcaggatgg aaagattata taaatatctg
    33301 taaagttgga ggaacaaaat catttttaga tattgtgagt atgggaaatt tgtattctcc
    33361 atttgatgat ggctgtattg agtctattat aggtgatgtt aaatcttggt ttgatgaaat
    33421 aaatgacagc aaattataaa aaattatgag aattttattg tataaaagtg aaaaattaaa
    33481 aaatatataa aaagtgatta tttgttgtga aaatagtctt gtttttaact aaattgataa
    33541 aataaactaa aaaagatttg tacttaaaaa aaaaagaaaa tacaagaaat atgcaatatc
    33601 aacatttgga aaaaaaagta aaaatgtata caaaatacat ataaaaaaac tattgcaaat
    33661 tttatagaat gtttatatac ttaagtcaac agagatagaa aaaagctatt tatagaaaac
    33721 aaaggataaa ttgcttaatt ttaaattttc tgaaaaaaat aataatccgg atgaagagta
    33781 tgggagactg ctttataaaa agcgaccgaa gggacaagtt taatcttact tacccaaaag
    33841 attagatgaa attctcaggt aaaggtgact gtacttggac ggggctctga aaatgatttt
    33901 aacagggaag acaaattggg agatttgttt ttcctgtttt ttatttatca agtaataaat
    33961 aaaagtttga aagagagtaa aaaaatgata ataattacga ataatccaaa agtaaaagaa
    34021 gaagttcaag gcagagaagt tttgtttaaa gacacaactt atattggaat cttagaagca
    34081 agtagagatt tgatacatga aggttatgaa cttttatctc atccactcta tgggagtgta
    34141 aaaccaaatg aaacacctta caggactgtt atactaaaga aaggaaatcg tttggatata
    34201 aactctttaa ccttaattga agaagcaatc atcactgcaa gtaaatttca ggataataaa
    34261 aagactccaa aatggacaga aaatgtccaa gacgatttta gagtaattga ttatgatata
    34321 ttttataata caatacaaag aatgcagtat gaataaaaaa atttgttaaa aagttattga
    34381 aacagcctaa atattaaatc taatttaata ttaaataaaa tgagaaattt aattggggag
    34441 gataagtcaa aatggaaaat gtgtatgact tagtaataat tggttcagga ccagctgggc
    34501 ttgcagcagg tctttatgga gcaagagcaa agttaaaaac tctaatccta gaaaaagaca
    34561 agactggtgg acaaattgta ataacacatg aaattgcaaa ttatccaggg tcagttccaa
    34621 atgcaacagg accaagttta atagctagaa tggttgaaca gtgtaaagag tttggagcag
    34681 aaatgctaag agataacatt gttgatactg agctagatgg agatataaaa gttttaaaag
    34741 gggaaaaggc agaatataga gcaaaagcag taataatagg aacaggagca acaccaagaa
    34801 agattggttg tcctggagaa aaagaactta ctggtaaagg ggtttcatat tgtgcaactt
    34861 gtgatgcaga tttctttgaa gattttgaag tatttgttgt aggtggagga gacagtgcat
    34921 tagaagaagc aatgtattta actaaatttg ctagaaaagt aacaattgta catagaagac
    34981 aaggatttag atgtgctaag agtgttgaag aaaaagcaaa agctaatcca aagatagagt
    35041 ttttattaga tacagtaatt gaagaaatca aaggtgatgg aatattagaa tcagttgtat
    35101 ttaaaaataa agttactgga gaaactcatg aatactttgc agatgaagaa gatggaacaa
    35161 tgggagtatt tgtgttcgtt ggattagatg ctcaaactga tttattcaaa ggtaaagttg
    35221 atatggatga aaaaggttac ataataactg atgaagatat gagaactaac ataccaggcg
    35281 tatttgcagc aggagattgt agatctaaga ctctaagaca ggttgtaact gcaactaatg
    35341 atggcgcaat tgcatctata gtagctgaga aatacattga tgaaaaattt gggaactaga
    35401 atctgtaaat taagaaatag tgtctgtaaa tttaatgaat aatatttaaa attaaaataa
    35461 aaatttcaag ggggaataag aaaatgttag atttagataa ggctacattt gaagaagaag
    35521 ttttaaatgc agaaggtttt gtatttgttg atttttggag tgaaggttgt gagccttgta
    35581 aagctttaat gccagatgtt cataaattag ctgagactta tggggataaa ataaaattct
    35641 gtaagatgga tacaacaaaa gctagaagat tagctataaa acaaaaagta ttaggtcttc
    35701 caactatggc tatatacaaa gatggagaaa aagtagacga agttactaag gatgatgcta
    35761 cagttcctaa tatagaaaac atgattaaaa aatatcttta atatgttgat aaagtattaa
    35821 cattaaaaga aaaaatctat ttttatctga agataagaag aaaaaggttt ttatgtccaa
    35881 ctttgttaaa aaaataattt taaataaaat aattttttta caataaatat tttttagtaa
    35941 taaaataaag atttaaggca taaaaacaca ataatatatc tttaaaaaaa taagataaat
    36001 tgtaaaatga tataattttg agaaaaactc aaatgcatat acacaaatta aataacagga
    36061 ggtgcaagta tgcgtcttga attagggaaa atctttataa aagatattca atttggtgat
    36121 gtaacagaag taaaagatgg agtattattc atcaataagc aagagatgct acaagaaatt
    36181 ggtggagatg agcatattaa atctatagat atagaattag ctcgtccagg tgaaagcgta
    36241 agaataacac ctgtaaaaga tgttattgag ccaagagtaa aagttgaagg taatggggga
    36301 atcttccctg gtatcatgtc taaagttgat actgtaggag aaggaaaaac tcatgcttta
    36361 aaaggtgtag ctgttgtaac tactggaaaa atagtaggtt tccaagaagg tataatagac
    36421 atgactggag aaggagctaa gtatactcca ttttctaaat taaataatgt agtagtaata
    36481 gctgaaccta ttgatggttt aaaacaatac gctcatgaaa aagctgttag aatgatagga
    36541 ttcaaagcag caatgtactt aggagaagta gcaagaaatc ttactccaga tgaagtttca
    36601 gtatatgaaa ctaaacctct tcttgaatca ataaaagaat atccagagct tccaaaagta
    36661 ggatatgttt acatgcttca gactcaaggt ttattacatg atacttatgt atatggagta
    36721 gatgcaaaac aaatagtacc aactttatta tatccaacag aattaatgga cggagcaatt
    36781 gtaagtggta actgtgtttc tgcatgtgac aaaaacccaa gttatgttca tataaacaat
    36841 ggggttgttg aagacttata tgcacgacat ggtaaagata taaactttgt aggtgtaatt
    36901 ataactaatg aaaatgttta cctagcagat aaagaaagat cttcaaactg gacttctaaa
    36961 ttatgtaaat atttaggatt agatgcagtt atagtttcac aagaaggttt tggaaatcct
    37021 gatactgacc ttataatgaa ctgtaagaag atagaaatgc aaggtgttaa aacagttata
    37081 gttacagatg aatatgctgg acgtgatgga ggttctcaat cacttgcaga tgcagatgta
    37141 agagctgatg ctgttgtaac aggtggaaat gccaaccaag ttgtagttct tcctaagtta
    37201 gataaagtta ttggacattt agaagtagta gatgtaatag ctggtggttc tgatggaagt
    37261 ttaagagctg atggaactat tgaagttgaa atccaagcta taacaggtgc aactaatgaa
    37321 acaggatttg gtcatttaac tgctaaagga tattaagaag tattttatta aacgattgag
    37381 taatgttaat aaaacatatt atagataatt ataataagtg ttatagatat ataaaaatat
    37441 attagaaata taataaggag gattattatg agtttactta gtaataaaaa ggttcttata
    37501 ataggtgacc gtgatggtat accaggacct gcgatagaag aatgtgtaaa aacagtagaa
    37561 ggagcagagg ttgttttctc atctacagaa tgctttgtct gaacagctgc tggggctatg
    37621 gacttagaaa atcaaaacag agttaaagat gctgctgata aattcggagc tgaaaatgtt
    37681 gtgattttac taggtgctgc tgaagccgaa gctgcaggtc ttgcagccga aacagtaact
    37741 gctggagatc caactttcgc tggaccactt gctggagttg ccttaggatt aagtgtttac
    37801 cacgttgttg aggaaccaat aaaatcatta tttgatgaaa gtgtatatga agaccaaata
    37861 agtatgatgg aaatggtttt agaagttgaa gaaatagaag aagaaatgtc tggtataaga
    37921 gaagaatttt gtaaatttta atgcctaaaa gcaaaagggg tgaaactaag agatgggaaa
    37981 actaaaagca gtacattata ttaatcagtt ctttgctggt ataggtggag aagaaaaagc
    38041 tgatactaag ccacatgtgg cagaaacttt acctccaata agtttacaac ttgataaatt
    38101 attaggtgaa gatattgaaa tagttggaac agtagtttgt ggagatagct atttcaatga
    38161 aaatattgat agtgcaagtg aagaagtttt atcaatggta aaaggttttg aaccacaact
    38221 tttcatagct ggtcctgcat ttaatgctgg tagatatggg gttgctgctg gaacaataac
    38281 taaagttgta aaagatgctt tgaatatacc agctttaaca ggtatgtatg ttgaaaatcc
    38341 aggagcagat atgttcaaaa aggatgttta tgtagtagaa acttctgatt ctgcagcagg
    38401 tatgagaaag gcacttccta agatagctaa acttgcagta aaacttgcta atggagaaga
    38461 aattggaact cctaaagatg aaggatacat agctagagga ataagagtta actacttcca
    38521 tgaagacaga ggttctaaga gagctgttga catgttagtt aagaaaataa aaggtgaacc
    38581 atttgaaact gaatacccaa tgccaaactt tgatagagtt gacccaagta aagctgtaaa
    38641 agatttatca aaatgtaaaa tagctttagt tacttctggt ggtatagttc caaaaggaaa
    38701 tccagataga atagagtctt cttcagcttc taaatatgga acttattcta tagcaggagt
    38761 tatggattta acagaagaga cttatgaaac tgcacatggt ggatatgacc cagtgtatgc
    38821 aaatttagat gcagatagag tattacctgt tgatgttctt agagatttag aaaaagaagg
    38881 tgtaattgga aaattacatg agactttcta tacaactgta ggtaatggta catctgttgc
    38941 aaactctaaa aaatatgctt ctgaaattgg agcagcatta gtggctgatg gtgtagatgc
    39001 agtaatatta acttctacat gaggtacttg tactcgttgc ggtgcaacga tggtaaaaga
    39061 aatagaaaaa actggacttc cagtagttca tatgtgtaca gtagtaccta tttcattaac
    39121 agtaggggct aatagaatag ttccaactat agcaatacct catccacttg gaaatccagc
    39181 gcttgaccct acagaagaaa aagccctaag aagaggcctt gtagaaaaag cattaaatgc
    39241 tttaactaca gaagtagatg gtcaaacagt atttgaaaaa taaaaataac acacacatat
    39301 attatataga taaaaatata aacacacact aaaaataaaa aataactata cacaaaaaaa
    39361 ataatataga tttcatgaat ggataaattg gagttattca ttcatgaaat cttaaaaaat
    39421 gccaaaatac ataaacaata atagaatcaa atatacaata aaatacaatg attagagctt
    39481 ttaataagtt taataagatt aaaaaataag tgttcataaa atttatggag gtgcacaaaa
    39541 tgacttatcc agttttaaaa ggggcaggct atgtacttat tcatactcca gatatgatag
    39601 tgcaaaatgg aagtacttgt acagttgaaa gagcaacaaa ttcagattca gaatttttaa
    39661 aagaagtaag taatcatata agaagttatg aagatgtagt taactacatg ccaaatcaag
    39721 tttatatagg aaatagaaga ccagaagaat taagagattt accaatgcca tggtgtgaac
    39781 aaaagattga gggaactaga aatggtaagt ttggagaaat aatgcctcaa gatgagttca
    39841 tagctttaat gcaaataagt gatgcatttg atttagtaaa attatcacaa gaatttattg
    39901 atgaagtaaa gccaaaaatc gaaaataact accctgaaat tgcaccattt gtaggtaaat
    39961 taaagggtga tgatatagaa gaaggtaaag aattagtagc aacacacata gctgaaggat
    40021 tataccatga tggaaaattt gttggttatg taaaaagagc acatgatgta gatgttaact
    40081 taaatgccca tacaatgttt gaaaatctag tagtaaaagc atctggagtt ttatctgcta
    40141 ttcaaatgct aagacatagc aaaatagacc ctgctgaaat agattatgtt attgagtgtt
    40201 cagaagaagc ttgtggagat ataaaccaaa gaggtggagg aaactttgct aaatcaatag
    40261 ctgagatagc aggacttcag aatgcaactg gttctgacac aagaggattt tgtgcagcac
    40321 caactcatgc attaattcaa gcagcagctt tagtaaaagc tggcatacat aaaaatgtaa
    40381 tggtagtagc aggtggagca agtgctaaac ttggaatgaa tgctaaagac catgttaaaa
    40441 aaggtcttcc tgtacttgaa gatgttgttg gaggatttgc agtacttgta tctgaaaatg
    40501 atggtgtaaa tccagttata agaactgatt taacaggtaa gcatactgtt ggaactggtt
    40561 cttcaccaca agcagttatg acagcactta taacttcagg tcttgataga gctaatttaa
    40621 aaataacaga tgttgatgtg tattcagttg aaatgcaaaa cccagatata actaagccag
    40681 caggagcagg agatgttcca gaagcaaact acaaaatgat aggggcactt gctgttaaac
    40741 gtggagactt agaaaagaaa gaattgaaag attttgtttc taacaaagga ttaccaggat
    40801 gggcaccaac tcaaggacat ataccatcag gagctcctta tataggattt ttaatagatg
    40861 atttaactac aggaaataga aatagagcta tgatagttgg aaaaggtagt ttattcttag
    40921 gaagaatgac taacttattt gatggagtat cttttatagc tgaaagaaat acaggtgtta
    40981 ctgaagagac ttcaggaata tctaaggatg aaattaagaa gataatagct gaatctatga
    41041 aaaaactagc attagatatg ttagaagaat aggggtgagg atatgtcaaa aaaagtaatc
    41101 gcagatgtat tcttagaagt agctaatgca atagaaagcg gcgaatttgg caaaaaagta
    41161 aaaattggtg ttactacact tggaagtgaa catggtgtag aaaacatggt taatggtgca
    41221 caattagcaa aatctaattt atttgatatt gtgcttattg gaccaaaagt ggaaactgac
    41281 cttgaagtag ttgaagttaa tgatgaaaaa gaaatgcatg caaaaatgga agaattatta
    41341 gattcaggat atattgatgc ttgtgttact atgcattata attttccaat aggtgtatct
    41401 acagtaggta gagtaataac tccagcaaaa ggaaaagaaa tgatacttgc aacaacaaca
    41461 ggaacaagtg ctactaatag aatagaggca atggtaagaa atgccattta tggtattgct
    41521 acagctaaat caatgggtaa caaatgccca aaagttggaa tacttaatgt tgatggagca
    41581 agacaagttg agaagtgctt aaaagaatta aaagataatg gatatgacat ggagtttgcg
    41641 gattcaatta gagcagatgg tggatgtgta atgagaggta atgatttact tgtaggtgca
    41701 cctgatgtta tggttactga tacattatct ggaaatatat ttatgaaagt attttcttca
    41761 tatactacag gtggagatta tgaagctcaa ggctttggat acggacctgg agttggagaa
    41821 gactatgata gaaaggtact tatagtatca agagcatcag gttctccggt agtagcaaat
    41881 gctttaaaat acgcttatga tgttgtaaag ggagatataa gtaatgttgc aagaaatgaa
    41941 tttgcaaagg ttaaaaaagc aaaatttgat gacattatat cctctcttac taaaaaagaa
    42001 gttaaagctg aaaaagtaga agttaaaatg ccagataaag aaattgttac tcgtcaaata
    42061 gctggtgttg atataatgga tttagaagat gcagtatctg agttatggaa aaacggaatc
    42121 tatgctgaaa gtggtatggg ttgtacaggt ccaatagttt tagtaaatga agctaaagga
    42181 gacttagcag ttgaaacttt agttaaagct ggatatactg caaaataagt acatttttta
    42241 tatgtaaata agaacatata gttataattt tattgattat aattatatgt tctttttatt
    42301 ttatatcttg tcaatattta tatattaaca caatccattt atttgattct ctagctaatt
    42361 tattatattt attcaattaa aattaagctt tctaactaaa taaaaactaa tgaagtatta
    42421 aaatgaaatt aataaaatat gaaaaaattt taggaataaa gtatctgtta gataaaaata
    42481 agattaatta gtgatataat ataattaaga taaaatttat tatttggagg taaaatcatg
    42541 agcagagtaa aaaatgtagt aggattgttg ggaactaaag gtgttgatgc actatatcta
    42601 acaaagaaaa ctaatgtaaa ctatataagt ggttttcctg atgaagaagc atatgcagtg
    42661 atttgcaaag atggaaactt tttagttact gatagtagat atatggagct tgcagaaaag
    42721 gtttgcaaag attttgaaat tataaattgg cataattttg atagaagtgt ggcaaaggca
    42781 gttaaaagtg tatgtgataa agtgggtata aagaaacttg ggtttgaaag aactaatata
    42841 gtgtttgata aatatgaaga acttaaaaat ctgattgaga aggataatgg agaattaata
    42901 cctacagaaa atattgtaga gactttgaga tatgtaaaag acaaagatga aatcaaaaat
    42961 acaagaaaag cttgtgaaat agcggataag gcattagaag aattgattcc acatataaaa
    43021 gctggagtta gcgaaattga actggctaca aagttagagt actttatgaa aatgaacgga
    43081 gcacaaaata tagggtttga aactatactg atatcaggag caaagacttc attgcttcat
    43141 ggtaagccta gtgataagat tatagaaaaa ggtgattttg tacttataga ctatggtgct
    43201 atgtataatg gctatatatc tgatacaact agaactttta tagttggagg agcttctgaa
    43261 aagcaattag aaatatataa tttagttaag gaagctcaaa atgtaggggt tgagaacatg
    43321 aaagctggtg tacatgcgac aattccagat gctgagataa gaaaagttgt caaaaaatat
    43381 gaagactatt actatcaagg tataggacat ggagtcggta gggatgttca tgaagaacct
    43441 ttcattggta attatggtga taaaatcata gaagagggat gtataataac aatggaacca
    43501 ggtatttatt tccctggatg gggtggagtt agaatagaag atactgtttt gattactaaa
    43561 aatggtccag aaagacttac taaattccct aaagatttga tgattttaga caaataattt
    43621 aatttatata tattttttta cataaaatat aacataatct cactattaag ctctatttac
    43681 atattatagt aaagagggct taattttatt gtaagaggtg atagtatata tgtataaaaa
    43741 acataaattt ataaaaaata taaaaaaagc ctggtataaa atcaaattaa ttgatagatg
    43801 ccttataatt gtaatggcaa tattaatgtt tcaatctata tataatttat ttgttaatga
    43861 agtaaattca caagatacta caactattga tgtagtagta agaaccactt cagcagctat
    43921 atttgggtat tttttaagtg ctaattttat aaagagacct ttaaggaagt atacaaatac
    43981 tgatgtatct tcaaatcttt ttatagataa taatttaccc ccaaaaggtc aagagtctag
    44041 tcaaaataat ataatgaatg tcaaaaatac cattggattt acttcagaag ctgataatta
    44101 tgaaaagaaa atacctatta ataataatga aggttttgaa gaaggagaaa ctagtgaatt
    44161 gcaaattatt atagctactg taatttgtat agcagcatta acagtacttt ttgttgtaag
    44221 aaattttaca actacaacat cagcttcttt aggtactatt tcacagatga gagattttgt
    44281 ctcaggatgc gtgggttttt tgctaggttg tccaagtaaa cctgattaaa aaagtattat
    44341 caataaaagg tattttaaaa agtatcagta taataatatt tagatttaaa tgtatacaat
    44401 tataattcta taacattcta tattgtattt ttatactgat actctaaact atactattta
    44461 ttaaaatatt ttaacattgt atttaaacaa aatattatat ctgtataata taaatttagt
    44521 ttatatgaat agatgccata tactcaataa aaagtttcat actatgagat atgtattgat
    44581 ttcttctcca aagaactcca atatttgcag taattgcacg ttctaatggt attgaacaga
    44641 gattttctgt attttgaaat atttctttat acaatattgt tgaagccaag tcattttcta
    44701 gtaaataacg tatggtagaa agttgatttg agtgtaaaat tatatttggc ttagcttttt
    44761 caagtctttt tgtaataatc ttatagtgat aagaaccttc agataagatt acaaaaggta
    44821 cattttttaa catataattt gatataatgg gatgttttgc caattcattt ttgggatttg
    44881 taactaagta caattctgtt ttatagattg tctttgaatc tatatcatca taaatatcac
    44941 tgtcaattat accaatacaa aaatcaagtg tagattcttc aatcatttta gctccaacaa
    45001 atgttggaac ttcaaaaatt ttaagctcta tatctggata aattacatta aatctaggaa
    45061 gtatttttga aagcaaaaac gtacctaaaa ctgtaggaat acctattttg agagaagctt
    45121 ttctcttttt tcctaaatca agtgcgtcac attgaaaatc ctcaaattgt tttataaaat
    45181 gttttgactt gtctaaaaaa atttttcctt caggcgttaa ttctatttta ttacctattc
    45241 tgtaaaaaag ttcaaagcca agttcttttt ctaattcttt aattgctaat gttatggatg
    45301 gttgagaaat atgaatactt tccgcagcct tagtcatgtt tttatattta cagacagttt
    45361 gaaaatagta taaatagttt atattcattt ggtattagtt cctttcattt acatattagt
    45421 gacaagtata taattaacaa tagcataagt tttgtatatt gttgtaaaaa aatagaatag
    45481 ttttaaacta tcatagcata agtttttgat attttcaatc tattaagaat agtgctatta
    45541 ttaaattaac aaaagagaat agctggtatg gtttagagta aatcaatcta tattacagtt
    45601 atatcaacga atatcaaaaa attatctatt agaaaataaa aattattttt acaattaaat
    45661 tgttaattaa atcataaaga aaaaagtgat attaacaaga taaaattttt ttaatcagaa
    45721 atgttaattt aataacaaaa tagagtattg gggagaaagg agaaatatat tttttaataa
    45781 tatttgttaa tttaataacg aaaaatgaaa gggagtgatt tagtgaagtt tgaattgatg
    45841 gattttttaa tgaatccttt tgtccttatg tttgctgcag taattacagg aattttattt
    45901 ggaaaaatta aatttgggaa gtttaatttt ggggtatcag gagcattatt tacaggatta
    45961 tttattggat ggcttgcata tagcttgggg aatctaataa tagcaaaagg agaaacagca
    46021 gcaggatata aggcagcaac tgtaatgatg gggaatggaa ttatttcttc tgacttcttt
    46081 gatttctttt taataatatt tgttgcagca gttggtctat tggcagcaaa agatatgaaa
    46141 gctgtattaa agaagtatgg agcaagattt gtaattttag gagttcttat aacttttata
    46201 ggtggctcta tgacttatgc aatgacttta ctatcaagtg ataaaggaag tagtgcttat
    46261 gaagtttctg gcgtttacac aggagcatta acaagttcgc ctggattagc tgctgcttta
    46321 gaaacagctg gtaaacatgc agaagatgta tcaaaagaat ttgaaaaagc ttcaataaaa
    46381 gataaaaaag aaattttaaa agtagtcgac ccagaaggca aattggatgt gaatacaact
    46441 acatcactga ctcaagagca aatagataag tatattgcat atgctgaagc tggtgttggt
    46501 ataggtcatg cagtcgcata tccttttgga gttttaatag taatcttagg tgtaaatttc
    46561 cttccaaaat tatttaggat ggacctaaaa gaagaaagaa gaaaatatga gaaggaaatg
    46621 aaggaagcaa gagatagtgt ttctggtaaa acgataccag aagttccatt taatataata
    46681 acatttttct tgacatgttt agctggatac ttagttggag gaatacatgt atttatggga
    46741 ccactagggt acttcacact aggcgcaaca ggaggttcac taattgtatc tctagttctt
    46801 ggatatatag gtaagatagg ggtagtaaat ttcagaatgg aagaaaaggt tttaaatatt
    46861 ttaaaacaaa tagggctagt atttttctta gcaatagttg gattaagata tggtggaaaa
    46921 gtagttgatt caattatgac atctggtatg cacctagcct tagtagcaat agcagtaggt
    46981 gttacagcta tgatgatagg attcttagtt ggcaaatacg tatttaaatt aaattggatt
    47041 ttattatctg gagctgtctg tggtggtatg acatcaacac caggtcttgg agctgctgtt
    47101 gatgctcttg atagtgatga cccagcagca ggttatggtg ctacgtatcc atttgcatta
    47161 ttaactaaag ttatattggt tatagtttta cacaaacttc caatgtaaat aagattttat
    47221 atggggggat atttatgaat aatgaaggag catttgaaga attaagtgga agattaagaa
    47281 ataaagaatt gtgtgattta ataatgaatg cagatgaagc tgctaaaatt atagaggatg
    47341 gcatggttat tggagttagt ggatttacac cttcaggcta tccaaaagca gtaccattag
    47401 cagtttcaga aagagctaaa agtggagaaa agataaaatt aaccgtatat tcaggagcat
    47461 ctttaggccc agaagttgat ggagcatggt cagaagcagg tataattgaa agaagacttc
    47521 cttatcaaac taattcaata cttagaaata atattaataa aggtgttgtt gattatatag
    47581 atatgcacct aagtcattct actcaatttt taaattatgg aactatacct aaagtagatg
    47641 tagctatagt agaagcactt gcaataactg aagaagggaa tattatacca actagtggga
    47701 taggtaattc tccatctttt ataaaaagtg cagataaagt aatagtagag ataaatcttg
    47761 caaaaccaat ggaaatggaa ggtatggcag atatatatat tacggaaaat ccaccaaata
    47821 gaaaaccaat agaaataaat catcctaagg atagaattgg aacaacatat ataccttgtg
    47881 gtttggataa aattgcagga gtagtaatta ccaatatgca agataagacc cgtccattgg
    47941 gggttgtaga tgaagcttcg aaaaaaatat caaataatat tattgctttt ttaagagaag
    48001 aagtaaaggc aggcagatta tcaaagaatt tacttcctct tcaatctggt gtaggaagtg
    48061 tagcaaatgc agtattatat ggattatgtg aatctgaatt tgaaaattta acttgttata
    48121 cagaggttgt acaggactct atgttggatt taataagaat gggtaaagtt acaatggcat
    48181 ctactacatc tgtaagtcca tctccagaag gattaataaa atttgaaaaa gatattgatt
    48241 tttttaagga taagattata ttaagacctc aagaaattag taacaatcca gaaatagcca
    48301 gaagaattgg tgtaattgca atgaacactg caattgaagt tgatatttat ggaaatgtaa
    48361 attctactca cataatgggg tcaaagatga tgaatggaat tggaggttct ggtgattttg
    48421 ctagaaatgg agcaataact atatttagta ctgagtcgat tgcaaaaaat ggtgatatat
    48481 catcaatagt acctatggta tctcacgtag accacactga acatgatgta atggtaattg
    48541 taacagaaca aggatatgct gatttaagag gtcttgcacc tagagaaaga gctataaaaa
    48601 taatagaaaa ttgtgcccat ccagattata aagaacaact tagggattat ttaaatagag
    48661 cttgtaaaag tggagcaaaa caaacacctc acatattgga cgaagcttta tcatggcata
    48721 gtaaatttat gagtacaggt acaatgaaaa aggctgaaac ttttaaaagt gcattataaa
    48781 atatttaata aattggaggt agattttaaa tggaaaaagc agtggaaaac tttgaagatt
    48841 taagtaagga atacattaat ggttatattg agagagcaag aaaagctcaa agagagtttg
    48901 aatgttatac tcaagaacaa gtagataaaa tagtaaaaat agttggaaaa gtagtgtatt
    48961 ataatgctga atatttagct aaacttgctg tagaagagac tggaatggga gtatatgaag
    49021 ataaggtagc taaaaataaa agcaaagcaa aagttatata taacaattta aaagataaaa
    49081 aatcagttgg tataattgat atagatagag aaactggaat aacaaaggta gctaaaccag
    49141 ttggagtagt agcggcaata actccttgta caaatccaat cgttacacct atgagcaatg
    49201 caatgtttgc acttaaggga agaaatgcaa ttattatcac acctcatcat aaagcaattg
    49261 gatgtagtac aaaaactgta gagatgatta atgaagaatt agaaaaaata ggagctccag
    49321 aaaatttaat acaaatactt gaccaacaat caagagaaaa tacaagaaat ttaatttcat
    49381 cagcagatgt agtaattgcc actggaggaa tgggaatggt aaaagctgcg tactcaagtg
    49441 gaaaaccagc acttggtgta ggggcaggaa atgttcaatg tatcatagat agagatgtag
    49501 acattaaaga agcagtacct aaaatcatag caggaagaat ttttgataat ggtatcattt
    49561 gttcaggtga acagtcagta atagtagcag aagaaatgtt tgataaaata atggatgaat
    49621 ttaaaaataa caaaggtttt atagttagag ataaagtaca aaaagaagct ttcagaaatg
    49681 ctatgtttgt aaataaatct atgaataaag atgcagtagg tcaatcagtt catacaattg
    49741 caaaaattgc aggtgttgag ataccagaag atacaaaaat aatagtaata gaagctgatg
    49801 gaccaggaga agaggatatt attgcaaaag aaaaaatgtg tccagttata tctgcataca
    49861 agtataaaag ttttgaagaa ggtgtagcta ttgcaaaagc aaatttaaat gtagaaggaa
    49921 aaggacatag tgtatctata cattcaaata cagttaaaaa tatagaatat gcaggagaaa
    49981 atatagaagt atcaagattt gttattaatc aatgttgtgc tactagtgct ggaggaagtt
    50041 tctttaatgg tcttgcacct actaatactc taggttgtgg ttcttgggga aataacagca
    50101 tctctgaaaa tcttgattat aagcatctaa ttaatatttc tagaattgct tattatatgc
    50161 cagaaaatga agtccctaca gatgaagaac tttggggata aaacttagac aaaaaatatt
    50221 cattctaaat taaagtagga atatatttta atttagaatg aatttaagac ataaattaaa
    50281 tttaaattag tttattggac taaatttaaa atattaagag acatatagaa gaaggaagta
    50341 ttataattta aaataaataa aaaaatctat aaaagagaag gagtggtttt attatggcat
    50401 taatgacagg agctcaatat attgaaagtt tgagaaaatt aaatactaag gtttatatgt
    50461 ttggtgaaga ggttaagaat tgggttgacc atcctatgat tagaccatct ataaattgtg
    50521 tagcagcaac ttatgactta gctcatgacc cagaatatgc agatttaatg acagttactt
    50581 caaatatcac aggtgaaaaa ataaatagat ttggtcatct tcatcaaagt gttgatgatt
    50641 tgataaaaaa agtaaaaatg caaagactat gtggtcaaaa aacagcttct tgtttccaaa
    50701 gatgtgttgg tatggatgcg tttaatgcag tgtattcaac aacatttgag tgtgataaag
    50761 cccatggaac taattatcat gataattttg taaaatactt aacttatata caagaaaacg
    50821 atttagtagt tgatggagct atgactgacc ctaagggaga tagaagtcta tcacctagtg
    50881 ctcaacctga cccagatatg ttccttcata tagtagaaag aagagaagat gggataattg
    50941 taagaggagc aaaggcacat caaactggtt caataaattc acatgagcac cttataatgc
    51001 ctactataag tatgactgaa gcagataaag actacgctgt atcatttgca gttccttcag
    51061 atgctgaagg tgtgtttatg atttatggaa gacaatcttg tgacaccaga aaattagaag
    51121 aaggtgcaga tgttgattta ggaaataagg agtttggagg tcaagaagct ttagtagtat
    51181 ttgataatgt atttgttcca aatgatagaa ttttcttatg tggagaatgg gatttctctg
    51241 gtatgttagt agagagattt gcaggatacc atagacaaag ttatggtgga tgtaaagtag
    51301 gagtaggtga tgtaataata ggagcagcag ctttagcagc tgattataat ggagcaaaca
    51361 aagcttcaca tataaaagat aaactaatag aaatgactca ccttaatgaa agcttatatt
    51421 gctgtggtat agcatgttca tcagaaggtc acaaaacaga agctggaaac tatcaaatag
    51481 atttacttct tgcaaatgtg tgtaaacaaa atgtaactag atttccttat gaaatagtaa
    51541 gacttgcaga agatatagct ggagggctta tggttactat gccttcagaa aaagacttta
    51601 aatcagacct taaagttgga acaagtggaa tgactattgg cgaagtatgt aacaagtatt
    51661 ttaaagcttc ttcagtggct tcaacagaag aaagaatgag aatattaaga ttcttagaaa
    51721 atatatgtct tggttcatct gctgttggtt atagaactga gtcaatgcat ggggcaggtt
    51781 caccacaagc tcaaagaata atgatttcaa gacaaggaaa cataaatcaa aagaaagaat
    51841 tagctaagaa aattgcggga ataaaaaaag aggaagcttt aaatttataa aaagttgata
    51901 tataaaggga aattatatat taatgtgtat taattaatga aaagatatgt agtataagag
    51961 atagttataa aattatggtg gtttttagct aagtaaagaa atttttaata aggagaattt
    52021 acacatgacc tgtggagtta gattttgtgg gggctgtaac cctagatacg aaagagggaa
    52081 ggctcttaca ataatcaaag agcacttcaa tggtagggta gattttagca tagcacaaga
    52141 gaacgtcgag tatgatttgc tacttataat tggaggatgc gcaactctat gcgcatccta
    52201 tcaacaatat aaagtgaaaa atgaatgtgt taagatgtgg gattttagcc atatagaaag
    52261 agttataaat gtgattgaat cagcaatttg tgaaacttaa aaaagctaaa tggggggatt
    52321 tcattgagtt ggcaagaatt gtatcaaagt aaattatgta gtgctacaga agctgtaaaa
    52381 cagattaaaa acggtgatac tgtagtattt gcacattgtg taggtgaacc tcctgcacta
    52441 gtagaggcta tgattgaaaa tgctgaacaa tataaagatg ttgagattaa acatatggta
    52501 agcttgggta gtggtggata tactgctaag ggaatggaag ctcattttag agtaaatcca
    52561 atgtttgtaa gtggaaatgt aagaaaggca attgaaaatg gagatggtga ttttacacct
    52621 gcattcttcc atgaagtacc aaagttgctt agagaaaaaa gattaaaatg tgatgttgtt
    52681 ttagcacagg taactccacc agatgaacat gggtattgta gcttaggaac atcagttgat
    52741 tatacttatg aagccattaa aactgctaga acagtaattg ttcaagtgaa tgaccagttt
    52801 cctagaacct atggtgaggt agtccatgta tcagagtttg actatatagt ggaaaaatca
    52861 caacctttat ttgaactaca acctgcaaag attggagaag tagaagaagc aattggtaaa
    52921 aattgtgctt cattaattga agatggttct actttacagc taggtattgg tgggatacca
    52981 gatgcagtta tgttattttt aactgataaa aaggatttag ggattcattc agaaatgatt
    53041 tcagatggga ctttggccct ttatgaaaaa ggtgttataa atggtaaata taaaaatttt
    53101 gataaagaaa aaatgacagt taccttctta atgggtacta aaaaattata tgactttgca
    53161 aataataacc ctgcagtaga ggtaaaacca gtagactatg tgaatcatcc agcaattata
    53221 atgaaacaac ataagatggt ttctataaat tctgctatac aagttgattt aatgggacag
    53281 gtagttgcag aggctatggg acttagacaa tttagtggtg ttggaggtca agttgacttt
    53341 atacgtggag tgtctatggg ggaagatgga aaggcaatta tagctatgcc ttcaataact
    53401 acaaaaaaag atggtacagt aatttcaaaa atcgtatcta ttgtggatga aggtgcacct
    53461 ataacgacat caagaaatga tgttgactat attgtaacag aatacggtat agcagaatta
    53521 aaaggaaaat ctcttagaga aagagcaaga aatttaataa atatagctca tccaagtgta
    53581 agagaaagtc tagcagtaga atttgaaaag agatttaagg agaaatatta ataatagagg
    53641 ggtgttaata atggaagcac ttagagtagt tccaaagata ttttattttg atacatttaa
    53701 agaatttaat gaagaattta aaataggaaa aaatgattta gtaattacta atgagtttat
    53761 atatgagcca tatatgaaac ctcttggaat agatacaaat ttaatttttc aagaaaagtt
    53821 tggaacaggt gaaccatctg atgaaatgat agactccatg acaaaggaaa tgaagaaata
    53881 caattttgat agaattattg cgtttggagg aggaacaata gttgatatat gtaagatatt
    53941 agcacttgat gttccagaaa agtctattga tttatttgaa ggagaagtat cacctaaaaa
    54001 agtaaaagag ttagtagtag ttcctacaac atgtggtaca ggtagtgaag taacaaatgt
    54061 agctattgca gagttaaaat ctaaacatac taaaaagggg cttgcagtag aagaaacata
    54121 tgcagactat gcagtgttaa ttccagagac agtaaaaggg ttaccttaca aattctttgt
    54181 tacaagttcg gtagatgctc ttatacatgc tattgagtct tatctttctc caaaagcaag
    54241 tccatttaca gagatgtatt ctctacaagc aataaaaatg attatggatg ggtataagaa
    54301 gatagttgat aaaggagaag aagagagatt taatcatctt agagactttg tacttgcatc
    54361 aaattatgca ggaattgctt ttggaaatgc tggttgtgca gcagttcatg cactatctta
    54421 ttcaataggt ggagctttcc atgttgcaca tggtgaagca aattatcagt tctttactga
    54481 agtatttaag atgtattcta gaaaaaaacc taatggaaaa attataaaat gtacaaaaat
    54541 tttagcagat gcccttgaat gtgaccctaa ctgtgatgtt tatggagaat tagaaaagtt
    54601 tttaaataaa ttaatagcta aaaaagcttt gagagaatat ggaatggtgg aaagtcagat
    54661 agatgaattt acagatagta caatagcaaa tcaacaacga ctacttgcta ataattatgt
    54721 agagcttagt agagaggaaa taagagagat atttgcaaat ctttattaat acaatttttt
    54781 atttatatgt atagtcaaca taaacaatgc taattatatt tatgtacaaa taaaaataag
    54841 ggaacttaaa ttttaattaa agttaagttc tcttattttt atatggagtt aaatataagt
    54901 atatttttaa tttaaaatat gcttatattt aagtctatta ctttatttga aacaatttgt
    54961 agaatttcaa attccttgac agtaataagt aagtaattgt ataatcaaac tgcaatatat
    55021 taataatatt agtgaaaatg aaaggattat aaaatatgta taaatcagaa aatttttcaa
    55081 aaaaatctgt attaataaat tcatttttaa gtaaattttt aaaaataatt ggatggggtt
    55141 tggtaagctt atttacatta tcagccttag tactttcaac tatttccatt ataattggat
    55201 gggctgtaat acttcctatt caagtatgtg cagtattttt acttatagga ggagtttgta
    55261 tttttaaagg taagagatta ggagaccaaa tttctcgtta taaaaaatat tgtacaatta
    55321 taaacaatag aaatattatt ccagtagaac ttattgcaga aaacacatct aaatcattaa
    55381 attttataat aaaagatgta caaaagatga tagacaaaaa ctactttata aatacttata
    55441 ttgataaaag aaataatcag attgtgttaa caaatgaaga ttttattcca ccaagatatg
    55501 aagatgtagc atatgaagtc aaagaagaag aaaagactga tgaatccaat gaagttgata
    55561 caattataaa taaaggaatg aattatctaa cacaaataaa agaagctaat aaaaatatta
    55621 aatcagaatc aatgggcgat aaaattgtac aagtagaaga tgttacaagt aaaatatttg
    55681 atgttgtaaa acatgaccca tcaaaactta ctcaaataca aaaatttatg gattattatc
    55741 ttccaaccac tttaaaacta ttaaattcat atcatacatt ggaagaacaa ggaattgata
    55801 gagaaaatat aacaacaact atggaaagta ttgaaaatac aatgaatacg attgtagtag
    55861 cttttgaaaa tcaattggac tatctgtttg aagatgaagc cattgatatt tctacagata
    55921 ttactgtttt agagaatatg ttagtccaag aaggattaac gagtggtaaa ttttaacttc
    55981 aatagaaaaa atattctgtt atttttaaac aattaattag tcataaagta taaaatgtgt
    56041 attatataag ttaatcagta ctttatgaag gaagaggaga gaaagataca atgaataatt
    56101 tagatgatat accagttatg ccaactttga cacttgaccc atttggagaa gaatcaaaca
    56161 ctaatgatat agataattct gacttattaa tgaaaaagga tgaaaaagac ccagaagaag
    56221 aaaaactgtc tgaatctgaa cgcaagatgg taaaggaatt tgcggaaaaa atagatatta
    56281 caaatacaaa tatgattttg caatatggag ctggtgctca aaaaaaggta gcaagttttt
    56341 ctgaaacagc tttaaagagt gtaaaaacaa gagatttagg agaagttgga gatatgctta
    56401 caaatctagt tactgattta aaagcatttt cagcagatga aaaagaacaa agtggttttt
    56461 taggaatttt taaaaaggca aataataaaa tttcaaattt aaaaactaag tatgatagtg
    56521 cagaggtaaa tgtggataaa gtaagtaaag agcttcaaaa gcatcaggtt aaaatattga
    56581 aagatatagc aatgcttgat aagatgtatg aattgaactt agcttactca aaagagctta
    56641 ctatgtatat tatagcagga aaacaaaaat taaaggatat gaaagaatat gaaatgccaa
    56701 agttgagaga aaaagctcgt ttatctggtt caacagaaga tgctcaatct gtaaatgata
    56761 tggtttcact ttgtgacaga tttgaaaaga aaattcatga cttagaatta actagaatgg
    56821 tatcgcttca aatggctcct cagataagat tggttcaaaa tagtaataat ctgatggcag
    56881 aaaagattca atcaactata gtaaatacca ttccattatg gaaaaatcaa atagtgcttg
    56941 ctcttggaat ttctcactta aatcaagcta tgaaagcaca gcgtgaagtt agtaatatga
    57001 caaatgagct tttattgaag aatgcagaaa ctttgaagat gggaacaata gaaacagcaa
    57061 aagaatcaga aagaggaatt gtagatatag acacaattaa gaaaactaat caatctctaa
    57121 tatctactat tgatgaggta gttaaaatac aacatgatgg tagaataaag agacaagaag
    57181 cagaagttga attatcaaaa atagaaaatg aacttaaaag taagcttctt gaatttacag
    57241 ttaaataaaa attatactaa tataaaaaga gactgtctca aaatagagat aagtctcttt
    57301 ttcattgttt aaatgattaa agtatattta tattataaat ttctattatc ttttaacata
    57361 acatcatgag ctccaccttc aactatacta gtagaagata caagtgtaag tttagcattt
    57421 ttttgaagtt caggaatgct aagtgctcca cagttacaca tagtagaacg aactttactt
    57481 aaagaaagag ttacattgtc tttcaaagaa ccagcataag gcacataaga gtcaacacct
    57541 tcttcaaaag aaagtttctt atctccacca aggtcatatc tttgccagtt tctagcacga
    57601 gcagaacctt ctccccaata ctctttcata taactaccat ttatattaac tttgttagtt
    57661 ggactctcat caaaacgaga gaagtatcta ccaagcatta taaaatctgc acccatagct
    57721 aaagctaaag ttatatgatg gtcataaacg ataccaccat cagagcaaat tggaatataa
    57781 atacctgttt tttcaaagta ttcgtctcta gcttgagcaa cttcaattat agaagtagct
    57841 tgtccacgtc ctataccttt ttgttcacga gttatacata tagaaccacc accaatacca
    57901 actttaacga agtcagcacc agcttcagct aggtaaagga atccctcgcg gtcaactaca
    57961 tttccagctc ctaccttaac tgtatcacca tatttttcac gaatgaaatc aagtgttatt
    58021 ttttgccact cagaaaaacc ttctgaagaa tctatacata aaacatcagc accagcttca
    58081 actaaagcag gaacacgttc agcaaagtct ctagtattaa taccagctcc aactacatat
    58141 ctcttagaag aatctaaaag ttcaagtgga ttttctttat gagaagagta atctttacgg
    58201 aataccatat acataagatt accattatca tcaagtacag gaagagaatt aagtttgtgg
    58261 tcccatataa tgttgtttgc ttcctttaaa gtaacatctt tgttagcata aactatagat
    58321 gacataggag tcataaattc actaacttta gtatctaaat ccatacgact tatacgataa
    58381 tcacgacttg caacaatacc caataattta ccagttgaag ttccatcttc agtaacagct
    58441 actgtagaat gtccagtttt ttcttttaag tcaagtatgt cttttaatgt gttatcaggt
    58501 ttaatatttg aatcacttac aacaaaacca gctttgtggg atttaacacg tgctaccata
    58561 gcagcttcat tttcaatagt ttgagaaccg tatataaatg aaataccacc ttctttagca
    58621 agtgcaatag ccattttgtc atcagaaaca gattgcataa tagcagaagt caaaggtata
    58681 ttcatataaa tatctgcttc ctcgcccttt ttaaatttag taacaggtgt ttttaaactt
    58741 acattagcag gtatacaatc agtagaagag aaacctggta caagaaggta ttcactgaaa
    58801 gtatgtgatg gtgtatcaaa ataaaacgcc attttttaat cactccttat atttttatac
    58861 tttttataat tgatagtatt aattataact tattctttaa ttttattaaa gtattttgtc
    58921 aaatattgag atttttgaaa aatttaaact attttaatta aatttgttta atatatattg
    58981 ccttataggg taaagttgta tattaaataa tgtttagtgt ttaatagtag ctaatttaaa
    59041 attaatcttt agtgattcat ataattacat ttgtatagtt tatattgata atattactag
    59101 agagt
//
LOCUS       DAELXX010000008        58159 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.008, whole genome shotgun sequence.
ACCESSION   DAELXX010000008 DAELXX010000000
VERSION     DAELXX010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 58159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58159
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.008"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(84..1091)
                     /locus_tag="KPH29_000502"
     CDS             complement(84..1091)
                     /locus_tag="KPH29_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HBG1466142.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLLYFFSNNLIYA
                     QNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNSEVDGYSDF
                     ANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTSEF
                     GAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENTDF
                     GIRGQINNFDISNLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKHPE
                     SKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGIFIKWM
                     L"
     gene            1319..2203
                     /locus_tag="KPH29_000503"
     CDS             1319..2203
                     /locus_tag="KPH29_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG1466143.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ADKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDKKNKIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            2405..3955
                     /locus_tag="KPH29_000504"
     CDS             2405..3955
                     /locus_tag="KPH29_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG1466144.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKEKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            3984..4619
                     /locus_tag="KPH29_000505"
     CDS             3984..4619
                     /locus_tag="KPH29_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBG1466145.1"
                     /translation="MKKFLNVLFSVCISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSEDDIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     LLVVFLVGIILSVKNKNIKILKTTSITLIIMPLLLTVPILLNFEKSFIIFHKLLFRND
                     YWIFNPNLDPVINILPEEFFFHSGMMILILILLASILLFVMYRLYKSFKIK"
     gene            complement(4730..4990)
                     /locus_tag="KPH29_000506"
     CDS             complement(4730..4990)
                     /locus_tag="KPH29_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HBG1466146.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFKYTDKGIKDCSSCMLPHKKDNYNYIMGKFQDLVKITSKK"
     gene            5207..6490
                     /gene="hflX"
                     /locus_tag="KPH29_000507"
     CDS             5207..6490
                     /gene="hflX"
                     /locus_tag="KPH29_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBG1466147.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTSESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYADLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGENGITLDVNFDEEDFNIYREYILEK"
     gene            6498..7175
                     /locus_tag="KPH29_000508"
     CDS             6498..7175
                     /locus_tag="KPH29_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HBG1466148.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEAGEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDEAIKSLE
                     NAKNLKK"
     gene            7261..7704
                     /locus_tag="KPH29_000509"
     CDS             7261..7704
                     /locus_tag="KPH29_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG1466149.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTNCVLAPEFQE
                     GGFYKVKDALDMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            8010..8654
                     /locus_tag="KPH29_000510"
     CDS             8010..8654
                     /locus_tag="KPH29_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBG1466150.1"
                     /translation="MSNYRTLHEFGTDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVVVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITNGTGKIILGEEFYLSEQNGEILI"
     gene            complement(8740..9117)
                     /locus_tag="KPH29_000511"
     CDS             complement(8740..9117)
                     /locus_tag="KPH29_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBG1466151.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(9117..9503)
                     /locus_tag="KPH29_000512"
     CDS             complement(9117..9503)
                     /locus_tag="KPH29_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBG1466152.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            9682..10437
                     /gene="nadE"
                     /locus_tag="KPH29_000513"
     CDS             9682..10437
                     /gene="nadE"
                     /locus_tag="KPH29_000513"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HBG1466153.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYRAEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            10520..11239
                     /locus_tag="KPH29_000514"
     CDS             10520..11239
                     /locus_tag="KPH29_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG1466154.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(11407..12411)
                     /locus_tag="KPH29_000515"
     CDS             complement(11407..12411)
                     /locus_tag="KPH29_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBG1466155.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQELILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQVIYPSIIYRQSSPAQNLL
                     "
     gene            12677..13573
                     /locus_tag="KPH29_000516"
     CDS             12677..13573
                     /locus_tag="KPH29_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HBG1466156.1"
                     /translation="MSKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNTYPDMNICIGMATVFGCPFEGLPKIEDAIRFAK
                     RLWENGIREINLADTIGIADPKQVRETISALVEALPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHVNIKSDSCNC"
     gene            13711..14973
                     /locus_tag="KPH29_000517"
     CDS             13711..14973
                     /locus_tag="KPH29_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466157.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPALAIIPAMAVPVAITVGYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKQII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            15049..16242
                     /locus_tag="KPH29_000518"
     CDS             15049..16242
                     /locus_tag="KPH29_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBG1466158.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LKRCEHYKALKPEIEKWSVKHTIKEAVDLISEAGVPAAPIYNLDCVTTDEHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
     gene            16871..17665
                     /locus_tag="KPH29_000519"
     CDS             16871..17665
                     /locus_tag="KPH29_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBG1466159.1"
                     /translation="MESVMENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDIAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            17759..19099
                     /locus_tag="KPH29_000520"
     CDS             17759..19099
                     /locus_tag="KPH29_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG1466160.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            19235..20467
                     /locus_tag="KPH29_000521"
     CDS             19235..20467
                     /locus_tag="KPH29_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBG1466161.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELINILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKNGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     ALIESGIVTMNKEACEIC"
     gene            20507..21649
                     /locus_tag="KPH29_000522"
     CDS             20507..21649
                     /locus_tag="KPH29_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBG1466162.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGTDIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGIGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASSTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            21660..22454
                     /locus_tag="KPH29_000523"
     CDS             21660..22454
                     /locus_tag="KPH29_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG1466163.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFAPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            22465..23487
                     /locus_tag="KPH29_000524"
     CDS             22465..23487
                     /locus_tag="KPH29_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBG1466164.1"
                     /translation="MMRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLA
                     DQLGKELEVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERK
                     KPEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMAT
                     IVCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEVLPQEKKSV
                     NLTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGT
                     TVRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEM
                     IESLNR"
     gene            23586..24962
                     /locus_tag="KPH29_000525"
     CDS             23586..24962
                     /locus_tag="KPH29_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBG1466165.1"
                     /translation="MKEQWYKDIFARILSMTDDGFIVVNTSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKDKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(25294..25638)
                     /locus_tag="KPH29_000526"
     CDS             complement(25294..25638)
                     /locus_tag="KPH29_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466166.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
     gene            26126..27136
                     /locus_tag="KPH29_000527"
     CDS             26126..27136
                     /locus_tag="KPH29_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBG1466167.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEESTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     VF"
     gene            27272..27760
                     /locus_tag="KPH29_000528"
     CDS             27272..27760
                     /locus_tag="KPH29_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG1466168.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEDGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            27936..29864
                     /locus_tag="KPH29_000529"
     CDS             27936..29864
                     /locus_tag="KPH29_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG1466169.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFILSNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFENLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
     gene            30039..30449
                     /locus_tag="KPH29_000530"
     CDS             30039..30449
                     /locus_tag="KPH29_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBG1466170.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            30471..30773
                     /locus_tag="KPH29_000531"
     CDS             30471..30773
                     /locus_tag="KPH29_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HBG1466171.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(30989..31681)
                     /locus_tag="KPH29_000532"
     CDS             complement(30989..31681)
                     /locus_tag="KPH29_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBG1466172.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMMAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(31707..32894)
                     /locus_tag="KPH29_000533"
     CDS             complement(31707..32894)
                     /locus_tag="KPH29_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG1466173.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRIPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSISYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            33230..35107
                     /locus_tag="KPH29_000534"
     CDS             33230..35107
                     /locus_tag="KPH29_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG1466174.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKTVYDEVIKIVPQSNDVGKKNESQEKQSIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSFIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            35419..36825
                     /locus_tag="KPH29_000535"
     CDS             35419..36825
                     /locus_tag="KPH29_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBG1466175.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLDFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNTVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            36853..37701
                     /locus_tag="KPH29_000536"
     CDS             36853..37701
                     /locus_tag="KPH29_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG1466176.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            38362..39054
                     /locus_tag="KPH29_000537"
     CDS             38362..39054
                     /locus_tag="KPH29_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1466177.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            39051..39986
                     /locus_tag="KPH29_000538"
     CDS             39051..39986
                     /locus_tag="KPH29_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1466178.1"
                     /translation="MKMDEQTIILFLFLLSIGLLFLSIFAFSKLGHIHKRLKDIEEIL
                     ADVQIGNENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIGQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVDDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQISVESEIGKYTVFTVSLPLI"
     gene            40134..41057
                     /locus_tag="KPH29_000539"
     CDS             40134..41057
                     /locus_tag="KPH29_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1466179.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRKFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAHAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            41041..41811
                     /locus_tag="KPH29_000540"
     CDS             41041..41811
                     /locus_tag="KPH29_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1466180.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVPKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVTVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPLLSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            41821..42603
                     /locus_tag="KPH29_000541"
     CDS             41821..42603
                     /locus_tag="KPH29_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1466181.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGIAVLLSLFSFLCTFILGLLFCNINISVTLIVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            42980..43516
                     /locus_tag="KPH29_000542"
     CDS             42980..43516
                     /locus_tag="KPH29_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBG1466182.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            43548..43937
                     /locus_tag="KPH29_000543"
     CDS             43548..43937
                     /locus_tag="KPH29_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBG1466183.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            43974..44360
                     /locus_tag="KPH29_000544"
     CDS             43974..44360
                     /locus_tag="KPH29_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HBG1466184.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGSQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            44487..45929
                     /locus_tag="KPH29_000545"
     CDS             44487..45929
                     /locus_tag="KPH29_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBG1466185.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVENESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLIDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            46226..47041
                     /locus_tag="KPH29_000546"
     CDS             46226..47041
                     /locus_tag="KPH29_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG1466186.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINNFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKRVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
     gene            47642..48826
                     /locus_tag="KPH29_000547"
     CDS             47642..48826
                     /locus_tag="KPH29_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466187.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
     gene            complement(49203..50312)
                     /locus_tag="KPH29_000548"
     CDS             complement(49203..50312)
                     /locus_tag="KPH29_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG1466188.1"
                     /translation="MDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
     gene            50775..51968
                     /gene="nifV"
                     /locus_tag="KPH29_000549"
     CDS             50775..51968
                     /gene="nifV"
                     /locus_tag="KPH29_000549"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HBG1466189.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVI
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            51993..53918
                     /locus_tag="KPH29_000550"
     CDS             51993..53918
                     /locus_tag="KPH29_000550"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HBG1466190.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREDAWIELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSNVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            53940..54935
                     /locus_tag="KPH29_000551"
     CDS             53940..54935
                     /locus_tag="KPH29_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HBG1466191.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGAEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            55262..55735
                     /locus_tag="KPH29_000552"
     CDS             55262..55735
                     /locus_tag="KPH29_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG1466192.1"
                     /translation="MVGSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDEFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            55769..56263
                     /locus_tag="KPH29_000553"
     CDS             55769..56263
                     /locus_tag="KPH29_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG1466193.1"
                     /translation="MIRKANMNDLESIMKIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            56360..56881
                     /locus_tag="KPH29_000554"
     CDS             56360..56881
                     /locus_tag="KPH29_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG1466194.1"
                     /translation="MIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMYDDNIEADKYIREELKVPSKYSVECIISIGYSDEEKKAYND
                     SDLDYKKVHFNNF"
     gene            57070..57381
                     /locus_tag="KPH29_000555"
     CDS             57070..57381
                     /locus_tag="KPH29_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG1466195.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            57409..58095
                     /locus_tag="KPH29_000556"
     CDS             57409..58095
                     /locus_tag="KPH29_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG1466196.1"
                     /translation="MKYSYKKCIIDSILLMFIIQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
BASE COUNT        21858 a         6718 c        10691 g        18892 t
ORIGIN      
        1 ctgtaggtta aataaaagta cctttagacc aaatatatta ttaataactt aataacatct
       61 taaaacttat ttatttatat tatttacaac atccatttaa taaatatacc ataacctaca
      121 gctgggtcat tttcaattgc atgtgacact gctccgatta ttttattatc ttgaactatt
      181 ggagtaccac tcataccttg tacaatacca ccagtttgag ctaaaagctt tttatctgta
      241 attttaattt taaaactttt actttctgga tgtttttggt tttctatatc tataatttca
      301 atattatatt ttttacactt attaaagtta tcttgtaaaa ttatttgagc ttttcctaat
      361 ttaacttcat ctaaagatgc tactttatat ttttttaaat tggaaatatc aaagttatta
      421 atttgacctc ttattccaaa atcagtattt tctgtaaagc taccaataat attttctcgt
      481 tttgcattta tacatccaac actaccttta taagattttt ctatattaag gtgcttagtt
      541 gtagaaattg aacctgtttt aataggtatt tttcttgcat taccaacact tattggatga
      601 cctacagctc caaactctga tgtatctgga ttgatatacg ttaaagtagc aaatccagaa
      661 agtaaattgt taaaactaat tttttctaat atgtgtttgg tagttttcat tgttataagt
      721 tgtccacctc tatttaataa aatagatact tgtttttctt ctggtaaagc atttaaggta
      781 ttggcaaagt cactatatcc atcaacttca gaattattta cctttactat agcatcaccc
      841 aatctaaatg gagatgcttc tccaggattt ctaactatga tattttttaa ttctgcgtca
      901 atttgaagta cgtttccaat tggtattaaa tattcaggtt catttttttg taaattttgt
      961 gcataaatta aattatttga gaaaaaatat aataaaaata aaaatgttaa aacaattgta
     1021 aattttgact tagctaagtt ggttttttta cttaaagtat tatttttaaa atgaaaattt
     1081 ttaagttgca taaaacacct ctttcttatt aaattatttt aataatagaa ttgaagtttt
     1141 cttgctgctt ctatattatg ttgtgcaaga attatgtttt gatgcttgga aaactgactc
     1201 aataacacct tagttttcat aaaaataaat aaaataaata gtgtatatga taaaataaat
     1261 acatattcat ataaataaaa ataaacaata actgtctagc ataagaaagg agcaaaaaat
     1321 gaaaaaatat agattgttag ttacttgtat aattgccatg ggaatcataa ttataggaac
     1381 atcaaatcca gttagcaatt tttacaagac tacaaatttg attagttcag gaaatatcac
     1441 aaatgaaaaa gcagataaag atgaaaattc aaacaaaaat aataaagata aaaatgaaga
     1501 caaatctaat tctaaaagta ataaagataa gtcatcagag gacaaatcgt cagataaaaa
     1561 aaataaaatt caaaaaaata aaaaactttt aatttgtata gacccagggc atcaaggaaa
     1621 aggagatagc aatttagaac cagtagctcc aggttcttcc tctaaaaaag cgagagtatc
     1681 ttcaggaaca gagggtattg ctacaaaaaa accagaatat gttctaaact tagaagcatc
     1741 tcttgtttta aagagtatat tagaatctaa aggttataat gtaataatga ctagagagac
     1801 acatgatgta aatataagta attcagagag agcaatactt gctaatgata aaaaagcaga
     1861 tatggttgta agaatacatg cagatagttt aaataattct tcaaaaactg gagcatctat
     1921 attgattcct gaaaaagatg gaaaatatac agctcctata tatgaagaga gtaataaatg
     1981 tgctgaattt atcaaacaga atatggaaca atcaggtata caaatcaatg gaatcgttca
     2041 aagaggtgat ttgactggat ttaactggtc aaaagtacca gccgtattag tagaaatggg
     2101 atttatgagt aactataatg aagaccaaat gatgtcaaat ccagactacc aaagaaaaat
     2161 gatgcagtgt atagctgatg gattggatgc gtattttaaa taagatatga atagagaaaa
     2221 agttaggttg gtattatttt cctaattttt atctctattt tttattgtac ataatgcaaa
     2281 gaagaagttt attttaggtt aaattgtcgt aaatataact aaaaatattg ggaatacttg
     2341 atttaagttt aaaaagaata tattatagta tagaatgtgt aaaatttttt tgagaggtgt
     2401 tgaaatgcta gtagtagaaa atgtgagtca tggatttggt gcgagaacaa tattagaaaa
     2461 tgtatccttt agacttagaa aaggtgaaca tattgcacta gttggtgcta atggagaagg
     2521 aaaatcgagt tttttaaata taataacaaa gaagcttatg ccagatgctg gaaatataaa
     2581 atggtcttca agagctacag taggttactt agaccaacat acggtattaa gcaaggaaaa
     2641 aactataaga gaagtgctta gagaggcttt taagcatatg tttgacttag agcaagaaat
     2701 gattgctatg tatgacaaaa tgggagaagc tagtgatgat gaaatgagta agcttcttga
     2761 agaaacagca gagatacaaa ctatacttga aaatagtggt ttttatatga tagatgcaaa
     2821 gatacaagaa gttgcaaatg gtcttggtct tggagaaatt ggtcttgata aagatgtaac
     2881 tgacttaagt ggagggcaaa gaacaaaagt acttcttact aaacttctac ttgaaaatcc
     2941 aactattctt atattggatg agcctacaaa ctatttagac gaagagcata taacatggct
     3001 tactaagtac cttcaagagt atgagaatag ttttgtctta gtttctcatg atattgaatt
     3061 tataaataat acatgtaatg taatatatca tatggaaaat ggagagttaa atagatataa
     3121 aggaaattat gatgaatttg taagacttaa tgatataaag aaacgtcaag aagaacaagc
     3181 ttatgataaa caagttgaag aaagaaaaag actagaggat tttgtagcta gaaataaagc
     3241 tagagtagca actagaggaa tggctaacag tagacaaaag caacttgata agatggaaat
     3301 attagaaaga ccaaaagaaa aaataaaacc tacttttgct tttaaagatg ctagagcagc
     3361 tagtaaaata atttttgaaa cagagaattt agttttaggg tatgatgaag cattgacaaa
     3421 accacttaat ttccatttag aaagaggaaa aaagattgcc ttaaaaggaa tgaatggtat
     3481 tgggaaatct actctattaa aaactttact tggaataata aaaccatttg aaggaaatgt
     3541 taaattaggt gactatttgg aagttggata ttttgaacaa gagagttcaa gggaaaatag
     3601 taatacacct atggatgaag tttggagtga atttccaggg cttacaaact ttgaagttag
     3661 acaggctctt gcaaaatgtg gacttacaaa tgaacatata acgagtcaaa tgagagtatt
     3721 aagtggtgga gaagctgcta aggttagact ttgcaaagta atgttaaaaa atataaattt
     3781 cttagtatta gatgagccta caaatcattt agatgttgag gcaaaagatg aattgaagaa
     3841 ggctatcaaa gagtttaagg gaacagttct tttagtatgc catgagccag aattttattc
     3901 agaaattgtt gatgatgtat ggaatataga agattttacc actaaaatcg tgtagtgatt
     3961 taacttattg ctggggggat ttcatgaaaa agtttctaaa tgtacttttt tcagtttgta
     4021 ttagtattgt tataatagta ggtgttatta attttactgt tggatttaaa caactttatt
     4081 attttgacat tgattactta aatatatcag agttatctgg tttatctgaa gatgatataa
     4141 aattgaacta cgattatcta attgattata acttaaataa gaatgtgtct gagttcaagt
     4201 taccaacatt aaaatcttct ccacaaggga agatacattt tgaagaagtc aggaatatat
     4261 ttcaaaatat aaataaatta gctaaattat tattggtagt ttttttagta ggaattatat
     4321 tgagtgtaaa aaataaaaat ataaaaatct tgaaaactac atcaataaca cttataatta
     4381 tgccattatt attaacagtt ccaattttat taaattttga aaaatcattt attatttttc
     4441 ataaattatt atttagaaat gattattgga tatttaatcc aaatttagac ccagttataa
     4501 atatattgcc agaggaattt ttttttcact caggaatgat gattttaata ttaattttgt
     4561 tagcaagtat cttactattt gtaatgtata gattatataa atctttcaaa ataaaataag
     4621 atagcaaaaa cgaattatat taaaggttat acaataaaaa tagctatgta ataatattta
     4681 aactaagagt agtcgataaa tatttatgca tagctatttc taatagaaat tactttttac
     4741 tagtgatttt aactaaatct tgaaatttgc ccataatgta attgtaatta tcttttttgt
     4801 gaggtaacat gcaactactg caatctttga tacctttatc tgtatactta aagtttccac
     4861 cacagttttc tcctaaagca tacaaaggac aatagcaaaa tagacagtta aactcttctg
     4921 gtttattagt tttatgacaa gggaaaaatt cgcaatcttt gtgattaaaa aacttataat
     4981 tttcgcccat attttcctcc taattaaata aaatttaaat tttttatact atataagatt
     5041 ttggtgataa tataattata ctaaataatt ttatgaatag tcgataatat agtattatta
     5101 atcatagtat taatggtata attagtcaac agaaaaataa tgtaaaaatt atgtaagtgt
     5161 attttgtaat tagaatatgt tttataacaa aaaatgaggt gattaagtga aagaaattca
     5221 agaaaaagca cttcttgtag gtctgaattt gactacaata gtgaaaaaaa atgatgatat
     5281 agacactagt gaatctatgg aagaattaaa agagctaaca aaagctgctg gggcagaagt
     5341 tgtaggtagc ttaattcaaa ataaacattc tgtagatgca gcttattata taggaaaagg
     5401 taaagtagaa gaaataagag cctatagtga ctctttagat gctactttag taatttttaa
     5461 tgatgaatta tctggtgctc aaatcagaaa tatagagaat gttgttggaa gaaaagtaat
     5521 agatagaact actttaattt tagatatatt tgctcaaaga gcacttagta aagaaggtaa
     5581 attacaagtt gaattagcac aattaaaata tagattgcca cgactttatg gaatgggtgg
     5641 tgagatgagt agaacaggtg cagggattgg tactagaggt cctggagaac aaaaactaga
     5701 aatagataag agacatatac ttaataaagc agcagacata aggcgtgaac ttaaagaagt
     5761 aaaaaagaat agggagacac agagagttaa gcgtttaaag tcaaatatac cgattgtagc
     5821 acttgttggg tatacaaatg caggaaaatc tactcttttg aacgaattaa taaagacaca
     5881 caaggattat gaacaagaaa aagaagtttt tgtaaaagat atgctttttg caactttgga
     5941 tgtaactcta agaaaggctc ttttgccgaa taaaaaagaa tttttagtag tagatactgt
     6001 tggatttgta agtaaacttc cccatgattt agtagaggcg tttaaagcaa cattggaaga
     6061 agtacagtat gcagatttaa ttttacatgt aattgatgcg acaaatacta gttatgaatt
     6121 gcaaaaaagt acgactgaag gtgtattaaa agaacttggt gtaaatgata aaaaacatat
     6181 acttgtatat aataaagtgg ataagctaga attggatata tatcctaaaa gtcaagaaga
     6241 tattgtgtat atttctgcaa aacaaggcat aaatatggat aagctattaa atatgataga
     6301 aattgcttta atggaaaata cttattcagt aagcttgatg ttaccttatg agagaggtga
     6361 tatatttagt agaataaaag ataaatataa tgtggagaat tttgagtatg gggaaaatgg
     6421 cataacgcta gatgtaaact tcgatgaaga agattttaat atctatagag agtacatact
     6481 agaaaaatag gttgaatatg gatataaatt gggaaaatat agataatctt gaagattatt
     6541 ttataaccta tcttctttat aaagaatcta aaacagtatc tcaaattagt aaaattagaa
     6601 atatttcaag tactgaagca ggtgagcaac ttatacaagc aaaattaaaa ataaaggaaa
     6661 tgcaaaaaga tgattttgag gcatctaagg atattttaga taaattttta gaactagata
     6721 aaattaagag acttgatttt atggatagct tagatgacga aaaaatggta tactttaaga
     6781 gaaaggtttt caaaagaatc cttgtggaaa aaaatgctga agatttaatt gttttaattt
     6841 gggcgacagg tgagctaaaa gatgatagat tcttaaagtt gcttcatcag ctcacaaacc
     6901 acagacactc agatattaga agaataactt attcagcaat cagaaagatt gaatcaccaa
     6961 gtagtagaga agtactgcaa aaaggtctct acgataaaaa tgcacaaact agacagtatt
     7021 gtgcaaaggc tctttcaaaa ctaggggatg aaaacagttt aaagatttta cagcagttga
     7081 aagacaagaa taagaatttt gaaaaagaat atgtgcttag agcttatgac gaagccatta
     7141 agtcattgga aaatgcaaaa aatttgaaaa agtaattttt tattaaagca atttgtttac
     7201 ttaatgatgt atttctagag tttaacaagg taataaataa attaatggga ggggtttcaa
     7261 atgatagtaa gacgttgtgc aggtggagta gtgttttatg ctaataaagt acttattgta
     7321 aaaaatgata ggggagaatg gacacttcca aaaggaaaga tacttggagg aggacttcca
     7381 tatgagagtg ctgttcaaag agttaaagta gaaactggca tagatgctaa aatgatagat
     7441 gtggctggcg atactatgta tgaatttttc tcaagaagta gacaacaaga agtttgtaat
     7501 gcaataatgt ggtatgttat ggaagcttgt aacactaatt gtgtacttgc tcctgaattt
     7561 caagaaggtg gattttataa agtaaaggat gctttagata tgttatcaca tcataaagag
     7621 caagctttag tagaagtatc ttataaaaag tttaaggaat taaagaaatt agtttcagag
     7681 ggcgatgatg ttccagcaaa ttaattgtga atttagagga ttctgaggaa gggatagccc
     7741 cttccttttt taattgaagc agttttaagc gtaaattata tagtctcttt aaattatgat
     7801 ttatcaattt agagctttag tagtttatat ttaaaatcag taagatatat ttaaaataaa
     7861 aattaaaaag tatacaattt tattatataa ttgtcttatg atattgatta aagaagtatt
     7921 aaaagtccat ttatttttaa atagaataat tttatagata tataatttga tgtgtatatt
     7981 agaaaatgat taaaatagga ggaaatacaa tgagcaatta caggacatta catgaatttg
     8041 gaacagatga aataactatt gaaaagtctg tttttattgg atatgctaag cctataaaga
     8101 gtgaagaaga agcattagag tttataaatg aaataaagaa aaagcataaa gatgcaactc
     8161 acaatgtgtg ggcttatact gtaggtaaaa atatgaatat acaaagatat agtgatgatg
     8221 gagaaccaca aggaacagct ggaataccta cattagaggt tataaaaaaa gaagatttga
     8281 gagatgtagt ggttgttgtt actaggtatt ttggaggaac aaaattaggt gcaggcggtc
     8341 tagttagagc ttatacaaaa ggtgctaaat tgggacttga agcaggtaag ataatttata
     8401 aggtaatgta tcaagaagtt aaggttaaga tagactatac tcaacttgga aaagtgcaaa
     8461 atgagttaat gaatttgggg tattttataa aagatactgt atatgaggat aatgttgaga
     8521 tagtagtgta ttcaagatta gaagatgtag agaaattatc agaaaaaatg attgatataa
     8581 caaatggaac aggaaaaata attttaggag aggaatttta tttgtctgaa caaaatggag
     8641 aaattttaat ataatctaaa aggttattag aatagttaat acctcaaata gccaattagt
     8701 atttttaagt gaaaagccat taaattgatt taatctaaat caatttaatg gctttttatc
     8761 ggattttgca cagaatcctt tttttacttc aaataaaccg actaaaagta aaaatatccc
     8821 aaatattttt tttaatatat ctgaagacag ataattagct aatagtgaac caaatacagc
     8881 tcctaaaacc ccaaacacag ctataggtat aaccaattta aaaattacat ttttattttt
     8941 tatatgtata gcaagtgcta taagtgtcat agggatagat gaaagtaaat ttacgctttg
     9001 tgctatttta gtgtctatgc cagcaagtaa aacgagtgca ggtataagga ttgttcctcc
     9061 acccattccc attcctccaa taattcctgc aaaaaatcca attagagcaa ataacattaa
     9121 aacaccatcc ttaatgctgc aattatcata attattccaa aagaaattct aagaaacttt
     9181 ccagtaacct tatttagtaa tttagctcct ataataccac caagaatact tccaactgca
     9241 actttaattg ttaagttaac atcataagta cctttagata catataaaac agaacttgct
     9301 gtcgttaaga atattattat agaaagagca gttgcatgag atttatgttc ttctacctta
     9361 acgatgtcat ttaatattgg aactaaaaga gtacctcctc cagaaccaaa aactccattt
     9421 ataaatcctg taaaaactcc aattatcgta ttttttaagt taaaatcaaa gtattttttc
     9481 tttaaatcat cagttttttt cataaaaatc accttctgaa atagttttac caaaataggc
     9541 aaattatatt tatataaaat aagattaaat ataatctttc atgtttgttt aattttgcat
     9601 aaaaaaaaac atattaaaac aacatattaa tatatggtat aatatattaa aacaaagtag
     9661 caaatttgag aggagataac aatgtcaaat ataaaaatac aaatagacaa aactgtagaa
     9721 tggcttataa ataaggttaa tgaggcaaat gcaaaaggat taattgttgg tgtatctgga
     9781 ggaattgatt cggcagtagt tgctaattta attaaaaagg catttccaga aaactctatg
     9841 ggagttataa tgagcataaa aagtaatcct caagatagag aagatgcact taaggttata
     9901 gagggatgtg atatagaata ccttgattta gaccttatag aaccacaaag tgctatatta
     9961 gacatggtag taggaaattt aaaagataaa catttatata gagcagaata tttaaagatg
    10021 acagatgcga acttaagagc aagagtaaga atgagtacaa tttatacaat tgctaacaat
    10081 ctaggatatt tagttgttgg tacagacaat gctgcagaaa tacacacagg atattttact
    10141 aaatttggtg atggtggagt tgatatttta cctattgcaa atttaactaa aggagaagta
    10201 tatgaatggg caaaagagct tggtgttcat gaagatttaa taaacaaagc tccttcagca
    10261 ggtctttggg aaggtcaaac agatgaagat gaaatgggaa ctacttacaa tatgatagat
    10321 gctgtgttag aaggtagatt ggatgaagtt cctaaaagag accaagagat aatagaaaga
    10381 cttcatagat taagtgaaca caagagaaaa acgcctgcac aacctcctaa gttctaattt
    10441 actatttata agatttgtga taaaatttac aagtgatgaa aattagaata attaattgta
    10501 cgtattaagg agtggaaata tgggacgtat aggtaatata attaatagaa aaggtaaaca
    10561 agatgctcaa agagcaaaga tatttactaa acatgctaga gcaatagcag ttgcagctaa
    10621 agaaggtgga gcagacccag agtacaatgc agcgttaaaa acagctatag aaaaagctaa
    10681 ggcagataat atgccaaatg ataatataga tagagctatc gctaaaggtg ctggagcagg
    10741 cgctggagaa gactatgaaa ctatagttta tgaaggatat ggacctggtg gagttgctgt
    10801 tatagtagaa actttaactg acaacaaaaa cagaactgct ggtaacgtaa gatactattt
    10861 tgacaaaaac ggaggaaact taggaactag tggatgtgtg tcttttatgt ttgataaaaa
    10921 aggacaaata ttagttggtt taggagatgg agtttcagaa gaagaattga tggatgttgc
    10981 attagaagca ggagctgaag atttcataac agaagaagat ggttatgaaa taataactac
    11041 accagaagat ttttctagtg ttcgtgatga gttaaaagct aaaggatatg agtttatatc
    11101 tgctgatgtg aagatgatac ctcaaactac gactgtattg actgaagaat ctcatttaaa
    11161 aatgatgaat aaattagttg atatgcttga agaagatgat gatgttcaag atatatatca
    11221 taactgggaa gtagaataaa tagtataagc atattgaaat ttataagcat tgaatcaatt
    11281 ttaacatgtg ataaatttta tagaataaga aattactaaa atagacatac gcacctctaa
    11341 cctatatttg cataataata tgtaagtata ggtaaggggt gtttttatat ttataggtta
    11401 agaaatttaa agtaaatttt gagctggact tgattgtcta taaataattg atgggtatat
    11461 aacttgttgc tgaataccat ttatacgccc agttactgta tcaataatca tcctaagact
    11521 acgttctgct gtctcttcca tttttaaatc tataactgtc aaaggaggaa tacttcttgc
    11581 cattagtcgt gaaggtccat tacttgctgc aaatatttga atatcctctc caattacaat
    11641 tccagattca tgaaatgcat tcatcatacc aagagctaca acatcatgat tacaaaaaat
    11701 tacttttttt agtgaatttt ttgcaagcat tttttgagca atatcatatc cactatctat
    11761 tttattagaa caataatata tagaatctga tagatgtatt ccatgttctt tacatgtttt
    11821 taaaaaagat ttacccctta aagtcataca tgtaaaggaa gttgggttga atacaacagc
    11881 aatatcattt ttaccatttt gaatagcatg taaagctgct atacgaccag tttcctcatt
    11941 atcaatagat acacttgaaa aaccaggtat ttcacgattt acaacaacta caggaatttt
    12001 aggtttattt tttactagat attctatatc atcagcattg atacctgtta aaacaattcc
    12061 atcataataa ttatttgcaa aaaagtattc atcatcacat agatgaccaa ctttgtaagg
    12121 tcgtatgatg atatttatgt ttaaatgttc taaagaaatt atgtggttta ttgcttgaag
    12181 tatattggtt gatgccaact caaatccctc atcctgccaa taaaaagcta ttttaatatt
    12241 attatgttta ataggagtag agcgtttata tccaagctta tcagctgaat ttaatataag
    12301 ctcttgtgta tgtagcggaa tcctgcgttc atttccttta ccacttaaga cgtacgagac
    12361 agttgctgaa gaaagaccac agtcttttgc aatatctttg attgttgcca tgataaaatc
    12421 ctccttaaat agttgcttaa atttgcttat accaataatc ttaacatata ttcaactaaa
    12481 gaacaatatt ctacaaggtg catattttac aggattagaa taaataaatt taaaaaaact
    12541 tcttacttat gttgacataa tttagtttat aggattataa tatgcttaaa taagcttatt
    12601 attaactcaa attaagcaaa gttaagcagg aacatgcaat gctagcttgc aatgtgactg
    12661 taaaggggga aaaggtatga gtaaggtaaa tatatttgaa gttggtccta gagatggatt
    12721 tcaaaacctg aaagagtttt tagaaactga taaaaagctt aagattattg atggtcttat
    12781 tgcttcaggg attaaacaac ttgaaattag ttcatttgta agcccaaaag caatacctca
    12841 gatgaaagat gcaaaagaag tagcaactta ttgtgtagaa aaatatcctg atgtgaaact
    12901 ttatgcatta gttccaaatt tatatggtgc taaagttgca tgggagtgtg gtataagaga
    12961 tatatcctat gttatatctt tgagtgaaac tcataataaa aaaaatataa atcgtaccca
    13021 tgaagagtct attgatgagc ttaaaaaaat aatgaatact tatccagaca tgaatatatg
    13081 tattggaatg gctactgtat ttgggtgtcc ttttgaaggt ttaccaaaaa ttgaagatgc
    13141 cataagattt gctaaacgcc tttgggaaaa cggtatcagg gagataaatc tagcagatac
    13201 aatagggatt gctgacccta agcaagtaag agaaactatt agtgcattag ttgaagcttt
    13261 accagagtgc agatggcagg tacatattca tgatactaga aatatgggaa tggttaatac
    13321 tcttgtggct attgaatctg gaattactac tgttcagtct acacttggag gattaggagg
    13381 atgcccattt gctcctggag catctggcaa tactgcaact gaagatttag tatatatgct
    13441 tgaacgtatg gggtatgata caggagttga ttttgttaaa cttcttgaat tatcaaagta
    13501 tcagaagagt tttgtaaatg gagtatatag tggtcatcat gtaaatataa agtctgatag
    13561 ttgtaattgt tagttctatt gattaatcga ttaaaaaaat atgttgatta aaaaaatatg
    13621 ctgattggta aaatagattt attgataaaa aagataaatt ataaaataca ttatctaata
    13681 aaacaaataa aaaaattgag ggggattagt atggatatta tagctttact tttattttta
    13741 ggagttatta ttattgcatt tgtacgtaag aataatgcag gtattttggc tctagctatt
    13801 ggtgtcatag ctgttagaat ttttggaatg gatgataaag aactttatgg agctataagc
    13861 aactcaatgt ttactacact tgttggtata acactactct ttgcaatagt gaactcaaca
    13921 ggagcattaa agcttgttgc agaaaaaatt gtagctgctt ctggtaaacg tgtttggctt
    13981 atacctatag cagtttttat agcaggattt gttgttgctg gtgttggacc tggggcaatt
    14041 ccagcacttg caattatccc agcaatggct gttcctgtag ccataacggt tggttataat
    14101 cctgtaatgc ttgcacttat aggtgaatgt ggattaatgg caggtcgtat gactcctatt
    14161 actccagaag gtcaaattat aacaaccgtt gcagaagggg ttggaattga aaatgttatg
    14221 cctactatct tagcctgtca aactctttct acaattgtat tttcaatagt attgtttata
    14281 attttcaaag gatataaact taaaaaacct ataaatgtta tagatagtaa gcaaatagaa
    14341 aaatttaata taaagcagat aatttctcta ataagcatag tagttatgat tgcttttatc
    14401 ataatttttg atatgaatgt tgggcttgca gcttttgcag tttcagctgt acttattttt
    14461 ttcaatattg ctgatgaagg agaatgtatc aaatcaattc catggagcac gatagtaatg
    14521 gttcttggag taggtgcaat gatgacaatc gtagatgcag caggaggtat tgacttgatg
    14581 agtaatgcat tatcatcact gatgaatagt aaaacagcta ctccaattat gagtatttca
    14641 gcaggactta tgtctatggt tagttctgct ttagcagtag tttatccaac tatgatgcct
    14701 atgtgtgttg atatagctaa ggcagttgga ggagtagacc cactagcact tattgctgca
    14761 gtcggtgttg gaggttctct tgctggtgta agcccacttt ctactggtgg ggcattgatt
    14821 ttagcagcta tgggttcaag taagaaagac tttaataagg aagaacaaaa taaggttttt
    14881 gtacagcttt tcattatgag tgcagttggc ttattggtta ttgcagtagt atcaatatta
    14941 tcatttaatg caataacaca tttaatgaac taattataat aactgaaaat aattcattaa
    15001 atttaatttt taatattaat caatataata taatttggag gggaagttat gaaaaaagga
    15061 gcattaagca atataaaggt acttgacctt acaagagttc ttgcaggtcc atattgtaca
    15121 atgatgttgg cggattttgg agctgatgtt ataaaaatag agatgccaag aaaaggtgat
    15181 gatacaagag gttttggacc ttttgtaaat ggagagagtt tatactatgc aaatataaat
    15241 agaaataaaa aaggtatcac tctaaacctt aaatcagaag aaggaaaaaa tatattttta
    15301 gaaatggtaa aacatgctga tgttgtagta gaaaattaca gaccaggtgt aatggaaaag
    15361 ttaggtctgg gttatgatga gttggctaaa gttaacaatc aaataattta tggagctgta
    15421 tcaggatttg gatgttatgg accatattca gaacgtccag gttatgatat tattgcacag
    15481 gcaatgggtg gtcttatgag tataacaggg caagaaggag gagaaccaac gagagttggt
    15541 aatgctatgg gtgatgtact aggtggaatg aatttaacta ttggtatact cacagcactc
    15601 aacgctagaa aaataatagg aaaaggacaa agagtagatg tttccctcgt tgattctgtt
    15661 gtatcgtctc tcgaaactgg tatacagaga tactttgatt caggacaacc tgaacttatg
    15721 ggtaatagat atgcttcagc ctatccttat gactctttta aggcaagtga tggaagtttt
    15781 gtaataggct gtggtaatga caaacttttt gaaaagttat gtaaaaaagt tctttttaga
    15841 gaagaccttt taatagatga gagatttaaa acaaatctta agcgttgtga acattacaag
    15901 gctcttaaac cagaaataga aaaatggtct gttaagcata caataaaaga agcagttgac
    15961 ttaatatcag aggctggagt tcctgctgct cctatctata atcttgattg tgttacaaca
    16021 gatgaacata tagcaaaagc aagagaaatg attgtagata ttagtcatcc tattatagga
    16081 aatatgaaag tcaatggaaa tccagttaag ttgatgacaa caaaagctaa aattgatatg
    16141 ccagcaccat ctctagggga aagtaacgag gagatttata atggttggct tggttttgat
    16201 agtgataaaa taaaagattt aaaagaaaat ggagttattt gattaaaaaa tagctaaaca
    16261 cacttgtgac tttagtcatg ggaggttcag ttagaataat atcaaaaaat attaaatcaa
    16321 tactgtaaat caaatataga attgtcagaa gcatttaaga tgggaaaagc tacttagaca
    16381 ttgagtcttt gtggcttttc ctattttcat taagaacttt atattatcag aatagatatg
    16441 tactaaacta aagtattaac aatttaaatt aatagaaaat aaactaccta attatgttga
    16501 ataaagacta ctttagtgaa aataaactta tagagatgct atttaaatta ttttaatgga
    16561 aatatcaaaa atgagactgc attctcaaaa atgagactgc gttttttaag tattttctcg
    16621 aaaatgagac aaaaatattg ttttatgagt tttattgaag aaaaattctt ctgtgaatta
    16681 caatttaatc aattttaaag ttgaaaatta ttaaaaattt agtgaaattt atgtatattt
    16741 cgtttatttt attaaaaata acaatttttt actaaaaata aagaacttca atggtgattg
    16801 gcatagtact tgctatttta tattaacaag aaatgtagaa aaaataacaa tgtataatgg
    16861 ggggaatatg atggaatctg taatggaaaa tttgaacaat cttaaggtgg aggttaagga
    16921 caaagtctgt gttattacta taaatagacc taaagcttta aatgctctta atagtgacac
    16981 tcttagggaa ctatcacaag ttattgatgt agtatcagaa aatgaggcaa tattaggagt
    17041 tattattact ggagaaggta aagtttttgt agccggtgct gatatacgcc agatgcaaaa
    17101 ctataaaagt gaggaaggca gaaaatatgc aggttatgct cagggaattt ttgataaaat
    17161 tgaagctctt gagaaaacag taatagcagc agttaatgga tatgcattag gtggtggatg
    17221 tgaacttgct atgagttgtg atattagaat tgcatcagaa aaggctatat ttggacaacc
    17281 tgaagttaat ttaggagtta ttccatgttt tggtggaact caaagattat caagacttgt
    17341 tggaactgga atagctaaag agttaatttt tacggggaga caagtaaatg cagaagaagc
    17401 taaatctatt gggcttatta ataaagtggt tccaagtgat ttacttttag aagagtctat
    17461 gaagatgatg aaccaaatag ttgaaaaagc gccaatagca ataagatatg caaaagttgt
    17521 tattaacaaa gggatagata tggatttaaa gaatgctctt gaactagaga aagatattgc
    17581 aggtttaact tttgctacaa gagataagca agaaggaatg aatgcattta tagaaaagag
    17641 aaagccagtt tttgaaaata agtagctatt ataactaaat tggaacaatg tagaatatat
    17701 tcaaaatcta aatttcgtga aaaaatacat atagtttaat tttaaaagga gttaatttat
    17761 gaatacggga gtaattataa gtttagtagg gattatcata gctctatctc ttctagttgt
    17821 aatggttatg aagggaataa atatttttat tatagcaatt gtatgttcat cattagttgc
    17881 tataacagga agcctaaatg tgtatgatgc acttaaagtg gattatatga caggatttgt
    17941 tggatttctt caggcaaatt tctttatttt cctaacaggg acattaatgg gaaaaataat
    18001 ggagattact ggtggagcaa aatctattgc taagatgatt gtaagatgga ttggtaagga
    18061 taaagcacta ctttctattc caatagcttg tggaatatta gcctatggtg gagtaagtgt
    18121 tttcgttgta agttttgcag tgtttccgat agctttagaa atttttaaag aagctgattt
    18181 gccacgaaga tttataccag cagctttgac ttttggatgc tcaacttttg ctatggttgc
    18241 accaggggca ccacaaattc agaatattgt tccagcatca acattaggga cagatattat
    18301 ggccggagca gtaaatggtt ttatatcatg tgcagtaatg tttatagttg gaagtaccat
    18361 tttgtacaaa atggtttcaa aagaaaaagc caatggtggt cattttatag cacatgaatc
    18421 agatatattt gatgaagcag aagcaactaa ttctaagaga gaaaatggtc ctaatggtgt
    18481 aattgcctta attccattga tagtgtcaat tttaataatt aatgtaaaag ttaatggaaa
    18541 agcgatagta ccaattgagg tgggagtatt tattggagct ttattagtat atttattgtt
    18601 aaataaatat caagataata ctaaaatagt tggacatgtt ggtgatgcat gtaaaactac
    18661 agtagtagca atatgtaata cttgtgctgt agttgcattt ggttcagtag taaaatcagc
    18721 agttggtttt gacttcatag ttaatgccat gactaatatt cctggtcctc ctattgcagg
    18781 ggctgcagtt ggaactacag ttattgcagg tatttgtggg tcagcttcag gtggactagg
    18841 aattgcagct ccacttcttg gaccagtatt tttagccaag ggtgtttccg ctgcagcatt
    18901 aactaggact atggcaattt catcagcagc attagattca cttccacaca atggatatat
    18961 tgttactgta acaaatggtc tgtgtaaaga aacacataag gatgcctata tgccaatatt
    19021 ctgggtaact gttttaactc cgtttattgg aaccatagta gcagtcctac tgtttacaat
    19081 gttcccggga ttgccataag gaaaagataa aaggtataaa ttatattttg atattataaa
    19141 atcaattttc aaaatatata caataaattt catgttttaa tactataaat atgtatttaa
    19201 aataacaaat tgaccatata aggaggaatt gaaagtgtta aataaaccat tagagggagt
    19261 taaggttatt gacctaacgt attttgtagc aggtccaggg acttctaaaa tattagctga
    19321 ttggggtgct gacgttatta aggtagagcc aagctttgga gacccaggaa gaaagacagg
    19381 ggcaacaatg acaatgccaa tagatgatta taataatcct ttttacagca catataattc
    19441 aaataaaaga ggtttgtcta taaatcttaa gagtgaaact ggaattgaaa ttatggataa
    19501 gcttttaagt gaagcaaacg tatttgtttc aagttataga acaggagcat taaaaagact
    19561 aggtcttgat tatgaatcat taagtaaaaa gcatccacac cttatctggg gtcagataaa
    19621 tggttttggc gattttggac ctgctaagga taatgcagga tttgatacag ttgccttttg
    19681 ggcaagaagt ggggctatgc ttgatatagc agaaaaagat acaagtccaa taaatccacc
    19741 tatagccttt ggagatgcaa caacttcttg tagtttatct ggaggaatat gtgcagcttt
    19801 gtaccagcaa gctaagactg gaaaaggaca aaaagtaatg gcatctttat ttgggcaagc
    19861 aatatggaat gcatcatctt tattagcatc tacacagttt agtgatgagt atccaaaaac
    19921 tagaaaaaat gctatttcac cagtaataaa ttcttatcgt tgtaaggatg gaaagtggat
    19981 attcttaagt attttagagc atgaaagata ttttgaggca ctttgtagat tatttggaag
    20041 agaagactta ataaaagaag agaaatttgc atcttcagta gaaagtaaga aaaatgctac
    20101 agaacttatt aatattttgg atggtgaatt tgcaaagttt actcaaaatg agatggttga
    20161 aaaacttact gctgctgata tagctcatga aaaaattcaa catgttaatg atgtagtgac
    20221 tgatgaacag gctattgcta ataactatat atatgagcat acaagtaaaa atggaaataa
    20281 gacaatgctt gcatccactc cagttaaatt tggaaatatt gaagttaaca tgacttgtga
    20341 tgctccactt ataggagaac attcagatga gatattgaga gaacttggat acagtgattc
    20401 tgatatagaa gctctaattg aatctggaat tgtaacaatg aataaagaag catgtgaaat
    20461 atgttaataa acatgtgaag tatgttaata aataggaggg aattttatgg attttagatt
    20521 aacagaagca caattaatgc ttcaaagagt agcaaaagaa tttgcagaga atgaaatagc
    20581 accaatagct gctgaaacag ataaaacagg tatttttcca agggagcttt ttagcaaaat
    20641 ggctaaaata ggatttaatg gcattggaac acctgtagag tatagtggtt ctggagggac
    20701 agatatagaa aaggtaattg ttgttactga gattgccaaa aaatgtgcag catctgcagc
    20761 aatattatca atacacacaa tatatgctca agctattttg aaatttggta ctgaggaaca
    20821 aaagaaaaaa taccttccaa tgatggctga aggtgggtgt gtaggtgctt tcgccttaac
    20881 tgaacctaat gctggttcag atgctgcaag agctgcaaca actgcaatta ttgatgaaga
    20941 aacggatgaa tatgttttaa atggaactaa gtgttttatt tctggtggag gtcaagcaga
    21001 atcattaata atatttgcac tgacagaccc atcaaaggga ataaagggaa tgtctgctat
    21061 cattgtcgac aaaggtactc caggtttttc aataggaaaa attgaagaaa aaatgggtat
    21121 tcatggttca gaaactgcag agttaatatt tgataactgt agagttccaa agtctaactt
    21181 attaggaaaa gaaggcaaag gatttaatat agctatgact tgtttagatg gagcaagaat
    21241 tggaattgga gctcaagctg taggaattgc tgaaggggct ttagaagaaa gtatcaaata
    21301 ttctaaagaa agagtccagt ttggcaagcc aatttcagcg ttgcaaggta ttcaatggta
    21361 tatagcagat atggcgacaa tggtggaatc agcaaaatta ttagtatatt atgctgcaga
    21421 tttgaaagct agaggagaaa aacacactaa agaagctgct atggcaaaat acaatgcgtc
    21481 aagtactgca agagaggtta caaatctagc ccttcaaatc catggtggat atggatatat
    21541 gaaggattat ccattagaga gaatgtacag agatgccaaa ataacagaaa tttacgaagg
    21601 tacttcagaa atacacaaag ttgttatttc aagagcagta ctaggatagg aggaaaagaa
    21661 tgaatatagt tgtttgctta aagcaagttc cagatactaa tgaagttaaa ataaataaag
    21721 agacaggtac tctaataaga gatggtgtac caagtataat caatccagat gatagaaatg
    21781 ctttagaaga agctctaaaa atgaaagatg aactaggagc agtgattaag gttataagca
    21841 tgggacctcc tcaagctaaa agtgctttaa aagaagcttt ggcaatggga gctgatgaag
    21901 cttatttaat ctcagataga gcttttggtg gttcagatac ttgggctaca tctacaatta
    21961 ttgctgctgc tatagaaaag gttggaaagt atgatgtaat attctgcgga agacaagcta
    22021 tagatggaga tacagctcag gttggaccag aggttgcaga atttttaggg ataccacaag
    22081 ttacttatgc aaaagaagta aaggttcaag atgacaaatt actagtaact cgttatacag
    22141 agacagggga ttatttaata gaagcaaaga tgccagtttt attaactgct ataaaagagt
    22201 taaacaatcc aagatatcca agtgtaaaag gaatcttaga agcttataat aatggagatg
    22261 cgaagattac tgtattaaca cttgcagatt tagatgttga tacaactcaa attggattaa
    22321 agggttcacc tacaaatgtt tataagtctt ttgcaccagt taaagacaag cataatgaaa
    22381 taatagaagg tataaataaa aaagaaaaag ctgagaaact aatagaaata ctattcgatt
    22441 tgaaactagt ataggagtgt taagatgatg agagcaaaag ttaatcaagg tataaattta
    22501 aatgattata atggtgtttg ggtaataggg gagcaaaggg aaggcaaaat taatcctgtt
    22561 acgatagaac ttattggtga aggaagaaag cttgctgacc aattaggaaa agagcttgaa
    22621 gttgtgatag ctggatatga agtagagaaa gaagtgaagg aattacttca ctatagtgtt
    22681 gataaagtat attacatcaa tgaccctctt cttaaagatt tcactactga tggatatgca
    22741 atttcaattg caaatttaat cgaaagaaaa aagccagagg ttgtattggt tggtgctact
    22801 tctataggaa gagatatagc gcctagaatt gcaggtaagg ttggaacagg tctaactgct
    22861 gactgtacaa aacttgaaat agattcaaca gataataagt tattacaaac aagaccagca
    22921 tttggtggaa atttaatggc cactattgtt tgtccaaaaa atagaccaca gatgtcaaca
    22981 gttcgtccag gagttatggc aaaagctgta agaaacgaat cagagactgg aattttagaa
    23041 gttgttactc cagaacttac tgaaaaaatg attagaacta gattagtaga ggtacttcca
    23101 caagaaaaga aatctgtaaa tctaactgat gctagaataa tagtatcagg aggaagagga
    23161 ttaaaaagag cagaaggatt tgagcttata aaagaattgg ctgataaatt gggggcagag
    23221 attggtgctt caagagcagc agtagattca gggtggatag aacattctca tcaagtggga
    23281 caaacaggaa ctactgttag accagaacta tacattgcat gtggaatatc tggagcaata
    23341 caacacttag caggtatgag tgattctaaa tatatagttg ctataaataa agatgcaaaa
    23401 gcccctattt ttagtatatg tgactatgga atagtaggag atttatatga aattatacct
    23461 gaaatgatag aatcattaaa taggtaactt ttagaaaaca ttgaatatgt agaatgataa
    23521 gtcttataat attgtattgt aagacttatt atttttgctg aaggttatag tggggagatg
    23581 aaacaatgaa agagcagtgg tataaggata tatttgcaag aatattatct atgactgatg
    23641 atggttttat tgttgtaaat acaagtggag taataataga tattaatgat aaatattgtg
    23701 actttttagg aaaagaaaga aaagatataa taggacaaaa tatacaaagt attataccaa
    23761 atacaaaaat gctagatgtt atgaaagata agtactgtga agaaggagct atacatcatt
    23821 attcaggtgg aaatacaaaa gaaaaaagtg taatagtaag taggtcttat gtagaaaatg
    23881 acaatggaga agtagttgca ggagtagctc aagttaaatt tagattgcaa tcatttgatg
    23941 tagctaaaaa attaatgtca gaatatatgg agctccaata ttataaagag cagtttaagg
    24001 ataactgcgg ttttgacaag ctcataggtg aaaatagaga cttcatagaa cttaagaaaa
    24061 caggtgtgaa agcttctaaa acaaactttc cagtactttt aacgggtgaa acaggcacag
    24121 gtaaagaagt ctttgctaga gcaattcaca ataatagcag taggtcagat aaacctatgg
    24181 ttagtataaa ctgcgctgca ataccagaag aattattaga gtcagagcta tttggttatg
    24241 acgaaggagc ttttacagga gcaaaaaagg gtggtaaaaa aggtaagttt ttagttgcaa
    24301 ataatggtac aatcttttta gacgagatag gtgacatgcc tttgaccatg caagcaaaac
    24361 ttttaagggt tcttcaggaa agtgagatag aaccagtggg aggtcttaaa actataaaga
    24421 tagatgtaag agtaatctct gccacaagaa agaatttaag taaaatggtt gaagagggat
    24481 tatttagaga agacctatac tatagattaa atgtaataaa cattcatatg atggaattga
    24541 aggacaggca agatgatatt ttattgctag caaattatat attaaacaaa ttaaatgttg
    24601 aatacaagga attgaaggtt ttaagtgata aggttaaaaa ttgttttatt aactatactt
    24661 ggccgggaaa tataagagaa ctacaaaatg taataaaaag tgcctatgct gtaagtgatg
    24721 atatggtcat tatgatgtgt gaccttccat ctaagatgga taatatcagc agagtagctc
    24781 aatgtaatgt ggatagcaat tgttcaattc atgaaatggt tgagaattat gaaaaaagct
    24841 taattattga tgtgttaagg aagtataatt ggaaatgttc taaggcagca gaggttatgg
    24901 gaatacacaa gagtctttta tataagaaaa taaaaaagta tgaaattgaa ttaaataatt
    24961 aagataactt gaattaaggc aatgctaaaa aaagcgccct tattcaaagc gaactgtata
    25021 aactgtttta tgcagttaaa acgtgtaaaa gtagatgtga agtactaatg tcaatcatta
    25081 gtacttttta ttgtatttat aattactatc tatatttcag tatcttttga tatctaagat
    25141 tattatgttt aatttaggat ataacaaaac cctcttagtt attgacagtt ttttatattt
    25201 taactgtcta ctaagagggg atagtttagt ttaaatttag agtaaatttt atctagcatt
    25261 tataaaaaat attttgaata gcttttttgt tatttagtca taattttcta cttctttatc
    25321 aagttctttg ccacaagaat cacacttata tatagcattg tatccataat catattcatc
    25381 aactattttc ttttgtctaa atacaccacc acactcacat aggaaaccaa tttttataag
    25441 tgtaacgata aattgtgatt ttataaattt tatatcatta catttatcgt tagtgtaaaa
    25501 tgtataaata taagagttat cgcactcatc tcttaattca aatctttcaa tatatgtgtt
    25561 attttttaat tcctcaaatt cttctttagt taaatatgat ttttgttgca attcatgtaa
    25621 acgcttaatt tggtccatta tttatatcct cccaataatt ttagtatttt aagcttagat
    25681 aaggaatcta agttttatta aatttatccc aaggaattat ttaaatcata tttagtttta
    25741 ttaattacaa catatataaa ttacacgaaa ataaaactaa ttgtaaaaat aatatcctgt
    25801 gaaaacctaa actactaacc ccttactaac ccgaaaacta aaaaaagaaa atatatccaa
    25861 atataagtga tatataaata aagatgtttt atataaattt aactttaaat attctgacaa
    25921 taatatatat aaatatatta tagttaaaat ataccacata ttttgggcat tgtatactgg
    25981 aattagagta attaatttat tttttaacat acaaatgatt ttatgaaaaa aaacaagact
    26041 caaaaatttt tatattcttt gatagaataa aattgagagt aaatttattg aattttataa
    26101 aatttataag gggtgagtct aagatatgca tgaagataaa aatgatatat ttgaatcaat
    26161 agatactgag gattttgata ataaaacaga agaagaatgt ttagagctag taagaaaaaa
    26221 tggattgaat ttaatataca taaaaaatca aacagaagag atatgtctag aggctgttag
    26281 acaaaattgt aatgcaataa aatgtgttca gaatcagaca gaaaaaatat gtatagaagc
    26341 agttaaacaa gattggagaa tgttagaatt tgtggaagag tcaacagaag aaatttgcat
    26401 gttggctatt aaccaagatg gaacagcatt aaaatatata gaaaatcaga cagaggagtt
    26461 atgcttaaga gccgttgaaa aaaatggtgc ggctttggag tatgtaaaag aacaaacaga
    26521 agaaatatgt atagaagcag ttagaaacag cgaatttgga ttggcaagag tgaaaaatca
    26581 aacagaaaaa atatgtatgg aagcagttaa gcattgtagc tataatctaa aatgggtaga
    26641 aaatcaaaca gaagaaatat gtatggaagc agtgagacaa aatgggcttg atttgaaatt
    26701 tgtaaaaaat caaacagaaa caatctgttt aagagcagta agacaaaatg gaatggcatt
    26761 agaatttgta aaggaacaga ctgttggtat atgcttaaag gcagtaagac aatatggaat
    26821 ggcattaaaa tttgtaaaag aacaaacaga agaaatttgt acagaagcta taaaacaaga
    26881 taaaagagca cttagttttg taaaaggaga taaggaaaaa tacaaggctc tatatgataa
    26941 taatgaacca tttgcaaaga gatatgttag aaatgtaata gaaaaagaaa acagagcatt
    27001 gattaaaaaa ggagaagaaa atgcaaaaat caaaattgca ggaaaacttt atgatagagg
    27061 catgtctttt gaagatatat cagatatagt tgagattgaa ataagcaagt taaaagtgag
    27121 tttaggtgtt ttttaaagta ataaatgtag gtgtttttta tgggaaatag ttaataaaaa
    27181 aataatcgac tagctctttg taggacagca aaattagtga tataatttga tgtataataa
    27241 attattttta ttagataagg gggaattaaa gttgaagatt acagtattat accaaacaag
    27301 aagtggaaac acagaaaggg tcgctaagct gatagaagat ggagctaaaa aggtggatgg
    27361 agtagatgta aagcttatga gacttgataa tattgattta gattatttaa atgaaagtaa
    27421 agcagtaata tttggaacac ctacttattt agcaaataca acttgggaag taaaaaaatg
    27481 gtttgatgaa gattcaaaaa aagttaattt agctggaaaa ttaggagctg tttttgctac
    27541 atgtgattat atatgtggag gtccagatgt tgcgatactt actatagtag gacatttgat
    27601 ggttaaaggg atgcttgtgt attctggagg tggttcttta ggaaagccat ttatacattt
    27661 aggacatgta cattcaattg aaggtccaga gttacaagat gagaaggcta taatatttgg
    27721 tgaaagaatt gctactaagg caaaagaact atttgcttaa tttctatatg cataataatg
    27781 attagagtaa atttagggtt ttaatggaga tttgaagtaa aagttataaa ataaaaaatt
    27841 ttccataaaa ttactcaaga tgaattataa tataattata aataaattat attattaaaa
    27901 tatttaaaat caaacgtcaa tagcgaggga agtttatggt atggcatata gaatatgaga
    27961 ttttttctgc aattatagtt atttttttaa tggtatactt ttttagaagt aaatttatac
    28021 ctactttaca aaacaaaata tattgtgcac ttttaatatt ctcattttta tttatacttt
    28081 caaatatttt aggttcattt tgtctaaata atatagacaa aattccaatt tttattactt
    28141 ttttattgaa tcagatatac ttattgttat tgccaatacc agcagctctt gtatcatttt
    28201 atgttatggc tattatttat caagatataa ggtatatgaa aaaaagatta ttatttttat
    28261 ctataccact tataatttgt ataatcttat ctattacaaa tccttttacg catattctat
    28321 ttggattgtc aaaagaaaca ggttatatac ggggttgtgg atatgttgca acttttgcat
    28381 cttttttctt ttatgcaata tattcagcat ttttatcttt taaatataga aaagcaatgc
    28441 atgaatcaaa aatatgggct attagaggtt ttcttatagt ttcaattgtg gcaattatct
    28501 tacaggcaat ttttttacag tatcttctta caggaactgc atgtgcatgt agtttacttt
    28561 tgacatatct ttctatgcag aatcgaggtc ttattattga tgaccttact ggtgtactca
    28621 atagacaatc ttttattcaa gagctagatt tgaatatcaa tactgaacaa tcaggattta
    28681 ttattacaat tgcgcttgat gattttaagt ttatgaatga aacttttgga acaaaaaatg
    28741 gagatattgc actaaaagaa gttggaaaat atttgattca gatacatgat gtaaaccatg
    28801 tatatagaat agggggagat atattttcta ttgttgtcaa tagtcaatta ggtatagaac
    28861 cagatgatat tatcagaaag atagaagaaa gatttaaaaa gccttggcta gtagaagaga
    28921 taagttttaa tttagcaact agtattgctg ttgtttatta cccagaaaat gcagaaacga
    28981 cagaagatgt aattgttgct atagatttct ctatacatga agcaaaacgt tctgagtcaa
    29041 ggcgagttgt ttatgcagat gcgtctataa gtgaaaagat aaagaagaaa catgttatta
    29101 aaaattgttt aaaagaagca ttaaaaaatg atgggtttga agtgtactac caacctatat
    29161 tttctaatag caggggcagg tttacttcag cagaggcatt acttaggcta gaacacaaag
    29221 agttaggttt tatacctcct gatgaattta ttccaattgc agaacaaaca ggattgatta
    29281 actcaattgg attagtagtc tttgagaagg tatgtagatt tatagctagt gatgagtttg
    29341 aaaatttaaa attggataat atagctgtga atctttctgt agtacaatgt atgcaaaaaa
    29401 acttagctga tgatttactt tatcttatga ataaatataa tgtatcacca actaaattta
    29461 aacttgaaat tactgagact gtggctatgg gctcttttaa tataattaaa gaaacaatgg
    29521 aaagattaat tgatttaggt gttaagtttg cacttgatga ttttggaatt gcttattctg
    29581 gagttactaa tatgctttct ttaccatttt cagtaattaa gttagataag agtttgattt
    29641 ggtctatgaa tgaagatagt aggcataagc ttacagttga gacgattata gctcttataa
    29701 ataaattgaa tatgaaggct gtagcagaag gtgttgaaac cattaagtgt gctgaagatt
    29761 taatagctat ggagtgtgaa tatttgcaag ggtattattt ttctaaacca gttccagaag
    29821 atgtttttaa aaatttgttg aaagaaaata cttttacaag ttaacaccct ttattttttt
    29881 aattacaact ttgttcattg acactgccag cgatatatta taaaattaac acaataataa
    29941 atattaaatt tattatacat attattaata ttaattaatg gagtaaagat ttattatatt
    30001 ttatttaatt atcctaaagt gagaaaggaa gatgtgacat gaataaaaag aaaatattag
    30061 tgccaattga tgggacagaa agaagtatgc attctttgga atttataaaa ggaatattta
    30121 aaaaagatga agttgaagta gaaataatga atgtaaaaga actagtattt attgatggca
    30181 tatccctggc tgaagagata aaaaattctg aaaatttagg aagaagaatc ttggataaag
    30241 cagcagagat aatgggagag tatgatgtta aagtacattt tacttttgga tatccaggag
    30301 atgagataat tagaaaagca aaagaagata atgtagattt tatagttatg acaaaatcta
    30361 ctaaaaaagg tcttacaaga atgataggtt cagtaacagc aagtgtagtt aaacaagcaa
    30421 aatgtatagt gatgattgta ccggagtaga attagaaagg tatttgaaat atgaataaaa
    30481 agatagtagc agtttgtgaa agtgaaagaa gtttaaattc tcttaagaca gctgcaaatg
    30541 atttaggatt tgatttagtt tatgaaattc aagaaaatga taatataaaa aatgaacttt
    30601 ctatttatga tatagaagat gcatctatag tgttatttgt tacaagtgat tctatagaaa
    30661 gcatagagaa gattgataga tttatagata gagaatttta tgaagttgat ccaaagtata
    30721 taatagagga tgcaaaatct atagtaagtg aaattttaat agacttaaat taatcgtggg
    30781 tgtcgcacta agaatagagc gacgcctttt tacatatttt acataagaaa taaagggctg
    30841 tctcaaacta tagataagac tctttttcat tgttgaaatg atgaaaatat atttgatttc
    30901 atacaaaaaa gagtgcctca taactaaaaa gttcactttg agacactcca tttttatatg
    30961 ttagttgcat ttaaggtaaa atagaagttt aatcatgttg aaataactga attacatctt
    31021 ctaaatgatt gtacattgat gtataaccta agtaagagtc tttactatca aatgccatca
    31081 ttatcttgtt atggtggcta atcatatcct taagagaatt tctatattta aaaccgaagt
    31141 attggttaga agaaactgtt ttaggtatta actcataata ttttataaaa attggattat
    31201 gagaagctaa aattatctct ttgtgaaatt gtaagtcagc atcataacat tcttctatca
    31261 aaccatgaga aaccttatcc aattcctcta ttgactttag gtaagtatat tttatttttt
    31321 gaagttcttt agatgtagca tttaaaattg ctagtttagt agcttcactt tcgataagtt
    31381 gtcttgctct taaagattct acataaaatt cttgtatgtt atgattttta tagatttcac
    31441 catcatcaaa taggtcaact tttttaaatc cttcaactat aggacttgag taaggcacag
    31501 ctctaagtat tccttcctta cagagtatat ttatggcatt tcttacaggt attctactta
    31561 tgttaaatag gtcagcaatc tttctctcac ttggtaattt actattaatt ttaaaagttc
    31621 catttagaat gtttaattta atataatcaa taattttttc agtggcattt aagcttttca
    31681 taaaaactcc taaacatata atttttttat gattgtatta tatcatagtt aagagttaaa
    31741 tctacttttt tataatatgt ataagacagg atagaggcca aaatagctat aatagccaag
    31801 ataaagtatc catattgtat tgaagtatat tccgatattt taccaactat cattggaaat
    31861 acagacattc ctatagcata aattgcttga aaaaaaccca ttgcagtaga tttctgatgt
    31921 tttggtacat ttttcataga ctcgctagta aggtaagaaa ggagtatacc tgtagatata
    31981 cctggtagca cttgtagtac aaatattata ttaatatttt ttattgttgg aactaaaaag
    32041 caataggctg aaactaaaat aaaaactaaa ggaatccaaa attttggccc atacttagta
    32101 caaaatttag tagaagcaaa gccagaagaa gcaacagcac acaccatgta agatatagaa
    32161 gatagaccta tgagggtaga actagaacct aagtttttaa gaatttgagt agtaaatgac
    32221 attgttgtag acatttgaat accctgttga attaatgcca taaaggaaaa aaatacaagt
    32281 cttttatctt tgcagataga taaatatttt ttaattgata ttttttcagg tatttttttt
    32341 gaattatgat tttttatatt taaggctaga atcgttccaa taactgaaga caatacactc
    32401 atgatacata aggctgtcat tcctaatttt tcataaaata aagtactcat aacaaatcca
    32461 atcaacattc ctagattgtt taccaatatt atcgtactcg tagctttttg ttgatgttct
    32521 gtagaaaaaa agttggtata gtgaaccatg taagaaatcc aagttgatga tgatattcca
    32581 gataaaatgt tagctataag aaaaccattg cctccaggag aaaagactct tataagagat
    32641 gctaaaccag caaagaaaat tccagacagt ataaatattt tatgtttatt tatagaatca
    32701 gcaaatactc caacaggaat ccttaataaa cattgtgaga ttccatatgc acctattatt
    32761 ataccaatca tatttgtaga tacattacat gcacttaaat atggattttg gaaaggtata
    32821 tatacatatt gtgaaaacca gaaaaatgta acaatagaaa gtaaaagtaa tttttgatta
    32881 gatatttttt tcatattatc ctccatatgg tatttttata atgcaaataa tataccattt
    32941 agatttttta atcaagtact ttctctatta aaaaaaattt aaatatataa ttattattta
    33001 caaaaaaatt atactttata gttgaattaa gaaaacgctt atgatactat aaagttaaca
    33061 aatttgaata tataacagta gacgtgttac tggtaatgca ggcaagatcg aatcaatttt
    33121 tatagattat ctatagagta ctatgggtat aatctattta tttggttcga tttttttgtt
    33181 taattggcca taaaacaaaa agcctatagt tgaaaatgga ggatttttta tggattataa
    33241 aaatatagct caagaaattc tactaaatgt aggaggaaaa gagaatgtaa atgaagtgac
    33301 tcattgtatg acaagactta gatttaaagt caaaagcgct tctaaagtga ataaggataa
    33361 attatctaaa actgaaggag taataactgt tgttgagagt atgggtcaaa tacaagttgt
    33421 aattggaaat aaagtaaaaa cagtatatga tgaggttata aaaattgtac ctcaatcaaa
    33481 tgacgtaggt aaaaagaatg aaagtcaaga aaagcaaagt ataattaatt ctatattatc
    33541 agcagtagct ggtatattta cacctacaat acctgctata gcaggggttg gtatgattaa
    33601 aggtatttta tcagttcttg ctatgtatta tatgaataaa aatggagtag atataaaaga
    33661 aacgcaaagt tatattatct taaatgccat ggccgattct atattctatt ttatgcctat
    33721 tatactagga tatactgcgg ctaaagtatt taatgcaaat aaaataatat ctatggtatt
    33781 aggagctaca ctttgttatc caacttttac tgctttgatg gcaggtgaag aatctgttag
    33841 atttttagga ttggcagtaa caaaagctac gtatacatca tctgttattc caataattat
    33901 tgctatatgg gctctttcat acgttgaaaa agtattagaa aagtatatac ctgaggtaat
    33961 taaaataata atggttccaa ctttatcatt ggttgtaatg ttacctgcaa cattattttt
    34021 atttggacca atcggaattt atataggaaa tgtcataaac ttttcttata agtatattta
    34081 tgaattaagc ccagcgttgt gtggagcatt tgtaggtggg ttatggtgtg tgttggttat
    34141 atttggtgct catagagcat tattaccaat aggtataagt gatgttgctc aaacaggaag
    34201 acagaattta ttggcatttg caggagctgc aaacttttca caagcagggg ctgctctagg
    34261 agtattcttt aaaactaaaa atcaaggact aaaaacaatt tctatgtctg caactataac
    34321 agctttattt ggtataactg aacctgcaat atacggtgcc aatcttagat taaaaaaacc
    34381 tatggtatgt gccgtaatat gtggtgcatt tggaggtgcg ataatgggaa tgggtggagc
    34441 ttatggtaat gcatttgcaa accaaggtgt tttaactata cctgtatatg ctgaagcagg
    34501 tgcattaggt ttcttaagtt atctaggtgg ttgtgctata gcattttttg gttcagctat
    34561 ttcaacttat ctagttggat ttgaagattt ggaagagtct tctaataagg aaaattcatt
    34621 tataaaagta gaaacgaagg atggtgttat tgatataact tcacctgtag agggcgaatg
    34681 tatagaattg tcagaagtaa aggatgatgt atttgcttca aaagctatgg gagaaggtat
    34741 agctgtgtta cctacaaaag gagtaataac agcccctact gattgtgaag tagcttcttt
    34801 atttccaaca ttacatgcaa taggattaaa attagataat ggagcagaaa tgttaataca
    34861 tgttggaatc aatacagtag agttaaatgg taaatacttt acaaaacatg ttaatcaagg
    34921 agatttagtg aaaaaaggtg ataaacttat atcgtttgat atagataaaa taaaaaaggc
    34981 tggttatgat gtaactacgc cagttatagt aaacaataca tttgattttg ggcaagtagt
    35041 ttcttgtaaa tctagttatg tttcaaccaa tgataatata atttcattag tattagctgg
    35101 taattaagtt aatttgacta gagataagta aattgattta aaaggaaata agtaaatcaa
    35161 caattaaata catctaaaag taagtttatt gcaatgaatt gttaaagaaa ataattaata
    35221 tggagtaaag ctattgcaat gaagtaacaa agaaaataat taatatagag taagtttatt
    35281 gtaatgaatt gttaaagaaa ataattaata tagggtaaat ctatctcaat gaaataacaa
    35341 agaaaagagt taatatagag tgaacttatt gcaatgaatc aacaaaaaaa ataatcaata
    35401 taaaaaggag aaatatttat gaatacagga tttccaaaag actttttatg gggagcatca
    35461 tcttctgcat ttcaagtgga aggagcttgg gataaagaca ataaaggaaa aaccgtagcc
    35521 gactataatt cttttaaaaa gtctcattta caggcagata ctaaagttgc atcagacttt
    35581 taccataatt atgaagaaga tattgaatta atgaaagaat tagggatgaa aacatatcgt
    35641 ttttcaattt catgggcaag gataatacca gatggtgaag gagagataaa ccagaaaggt
    35701 ttagactttt ataataagat aatagacaaa ctaatagagt gtgatattga gccattcgta
    35761 actttatacc attttgacct tccatttaaa ttagtagaga aatataatgg ttgggaaagt
    35821 agagaaactg tctatgcttt tgaaagattt gctaaaatat gctttaagca ttttggagat
    35881 agggtaaagt attggcaacc tcataatgaa cagaatttaa ttgttagagt ggaggagaga
    35941 attaatatat atgatgaaac agattcatgg aagatagata aaatgcgtgc tcagatggat
    36001 tataatcttt gcttagcaca tgcattagca gtcaatgcat gtcatgagat gataaaagaa
    36061 agtaaaattg gagcagcagt atcatcatct gtaacatatc ctcttacttc taagccagaa
    36121 gatgtatatg cagctagaat gaatgataat tttaaagtgt actatatgct tgatatgcat
    36181 cattatggag aatatccagg gtattatatg aagtatcttg aaaaaagaaa tacagttcct
    36241 cacatggaag atggggataa agagatactt aaaaaagcta aaatggattt tatagctgta
    36301 aattattata ggacaaattg tgcagaagct ttacctgaag attcacaaca tccatttgga
    36361 ttaagagaag gaactgttga ttttagtatg tatggactat ttaaaatgtc aatgaatcct
    36421 aacttagagg cttctgagta tggagcagca atagacccat caggtcttag agttgcctta
    36481 aatgaatatt ggcaaagata tcacttacct gttataataa cagaaaatgg tctaggagct
    36541 aaagatatat tagaagatgg aaaaattcat gatgactatc gtatagacta tttaagaagt
    36601 catataaatg catgtaaact agctattgaa gatggtgtag agatgattgg atattgtcca
    36661 tggtcattta cagacctttt aagttcttct caaggtttta ataagcgtta tggacttgtt
    36721 tatataaata gaactgacca tgaagtttta gatttaaaaa gaatcaagaa agatagtttt
    36781 tactggtata aagaagttat agagaacaat ggaatagtaa aataaacata aatatatatt
    36841 gagggtttta gtatgtacat tacaaaggta ttaaataatt cgttgctact tgcaaaggat
    36901 gataatgatg aagaaataat attgatgggg aaaggcattg gccataatta taaggccggg
    36961 tctgagttaa ataaaaaaga tatagaaaag atttttgttc ttcatgatga aaatataaaa
    37021 aagagtatta tacaattggc aacagaaata gatgcagaat attttggtat agctcaaatg
    37081 ataattgcat atggaataga aaaatataac ttaaaactta tgaaccatat atatttggcc
    37141 ttaactgacc acattgcctt tgcagtgaga cgctttaaag aaggtataat gattgaaaat
    37201 cactatttgt tcgaaatcaa ggaatttaat ccaaaagagt atgatatagg aaaatatgct
    37261 attactgttt ttaaggaggt tcttggttta gacctaccag aagaagagat tggtaatata
    37321 gcaggccatt ttataaatgc acaacaagat aatccttata gtgatagaaa taagagaagt
    37381 gctaaaattg taaatgctat tttgcaaata gttcattatc acttttctat tgtatataat
    37441 aaagagtcat tttattatag gagatttgta atgcatctaa aggccttttc acaaagattt
    37501 ctggctaatg aaccatcaag agaaaaaata gattttatat atgagcaagt tcaaaaaaat
    37561 tgtaaggaag agtatgaatg tgtgaaaaag gtaggagctt atatgtataa ggaatactcc
    37621 agagaattac caagacaaga ggagttatat ttgatgattc atattcataa gattttagga
    37681 gaattatgcg aaaatagtta gaatatctta atatgctagt aatattataa aaaattaaaa
    37741 attaactaca ctttaaatat gaacaattgc attacttaag ggggttgtct taaaattgat
    37801 ttttttaatt aaatttgaag ctcaactcct tttttaaacc tttatatcat tacaacttta
    37861 atgtttatac gcctgtactt tagatgtata tctacttttt acatttctca acatgcaaac
    37921 ctaccatttt ttgcagtatg ttaattgaaa taaaccattg agttaaccct aaatttattc
    37981 tggacttata ggaatagttc caaagatact ttaaaatatt ttctggaata attgcttatt
    38041 tcacatatgt tttattgtga tgttttgatt taataaattc ttcaactctg tatatagtaa
    38101 atgtattatc atgtttgttt tattcaagca cacttataat acgcttgatt ttttcacaat
    38161 gatgaaatat actaatgggt gttataggta taagaatcga attacacttg aagaagtaag
    38221 tgataaatga gatataaatc ctcatatgat aaactactat ttcattgagg aacatattct
    38281 aagtgatgtc aagatactac aatatgacta aattcctagt gataaaaaag tctacgaatg
    38341 agtgtagaaa ggggaactaa gatgaataaa gttttaatta tagatgatga taaagatctt
    38401 tgtattttgt taaaaaagag tatttcaatt gaacagattg aagcaaacta ttgccattct
    38461 gggagagatg gactggttga actttcaaaa caagattatc agttagtagt tctagacgtg
    38521 atgatgccag gtatggatgg ttttgaaact atggaacaaa tacgaaaata tagcagtatt
    38581 cccatcttaa tgctgacatc aaaaagcgat aattacagca aagtgcatgg attgcgagct
    38641 ggtgcagatg actatctaac caaaccattt gaaattacag aatttactgc tcgtgtactg
    38701 tctttaatcc gacgttacac ccgttttaat ccaaaaaatg aggaaccaca gcaactttct
    38761 ttcaaaggtt tgttgattga ccttgaccag catacagtga ctaccaatgg cgaacctatg
    38821 ttgcttccac ccaaggaatt tggtgtgctt ctttactgtg caaggcatca gggtaagatt
    38881 ttgacaaaac agcagattta tgaagctgtg tggggtgagc catatgtata tgatgacagc
    38941 aatatcatgg ctattatcag tcgcctaaga aaaaaaattg aactagaccc atcatctccc
    39001 atctatttgc aaacaataaa aggaattggg tatcaattca acacggaggt atgaaaatgg
    39061 atgaacaaac gataattctt ttcttattct tgctgtcgat tggtctactt tttctttcca
    39121 tatttgcttt ttccaagctt gggcatattc acaaacgcct aaaagatata gaggagatac
    39181 tggctgatgt acaaattgga aacgaaaatc gcaaaatact aattaagcca tgtgatgtta
    39241 tggctccatt ggtttatcag cttaatgaga ttgtctacga ttatgaaaat aagttacttt
    39301 ctctgaaaaa gtcggataaa gctagcaaac aacttatgac aagtctatct catgatgttc
    39361 gtacaccatt aacaactctg attggttatc tggatgctgt acacagtggt atagttatcg
    39421 gacaagaacg ggaggaatac cttgaaatcg ctaggcgtag agcctacgac ctcaaggatt
    39481 acattgatgt tctttttgac tggtttcggt tgaactctga tgaatttacg ctgtcaatag
    39541 aatctgtgga aattgcagag ttgtcacgca atattttgaa agactggatt cctatattcc
    39601 atgaaaagaa actgaatttt gaaattgata ttccagaaaa tcgactaatg gtaaatctcg
    39661 acccagatgg atattcccgt gtggttaata atctggttca aaatgttctt gctcacagta
    39721 aggctcggca aatcaagatt accatgagtg aggattccag aatggtatta ctccgtgttg
    39781 atgatgatgg tgttgggatt gccagagaaa atttaccaca tatttttgag cgactgtata
    39841 agtgtgataa aggacgttca gaaaaaggaa gtggtcttgg tctatctatt gtcagtcaaa
    39901 tggtggagcg aatgggtggt cagatttccg tagaaagtga gattggaaag tataccgtat
    39961 ttacagtgag cttaccatta atataaaatt gtttttcccc catgtgacat gtatacacag
    40021 ggggattttt tgtaagttat aaagttgcaa ggttaataca aggttgttgc aaggttaatg
    40081 taagattgag gtggtatgat agctttaata aacaaaggag gattttatta tttatgaata
    40141 atttaattat tgaaacaaaa aatctcacta agcaatatgg tgctcaaaag agcgtttcta
    40201 atttgaatat tcacgttcag aaaggtcgca tttacggatt gctaggaaga aacggtgcgg
    40261 gtaagacgac aactatgaaa atgttattgg gtctaacaaa accaactact ggagaaatta
    40321 aaatatttgg tcaggacatt cacaataacg agaagaagct tcttcctcga attggatgtt
    40381 tgattgaatc accagggttt tatcctaatc tgacaggaac agaaaatctc aaaatctttg
    40441 caaggcttag aggaatacca aaaaaaaatg cagtagaatc agcactggat gtagtcggtt
    40501 tgccaaacaa tgacaaaaag ttattttctc aatactctct tggtatgaaa cagaggttgg
    40561 cgattgctct tgccattatg catgacccac aactgttgat tttggacgag cctatcaatg
    40621 gacttgatcc tattggtatt gcagaagtaa gaaagtttat tcgtgatctc tgcacagaac
    40681 gaggtaaaac tattttgatt tccagtcaca ttctttctga aattgccttg ttggcagatg
    40741 acattggtat tattgaccat ggtattttgt tggaggaaga aagtcttgcc caattagaag
    40801 aaaaaaacag taaatacata cattttgctg tatctgatgc agcacatgca gcaaggcttc
    40861 tggaaaccca acttaacctg aaaaatttca aagtagcaga taattacaat attatgctat
    40921 atgatacaga tgtttctgtt gcagaaataa atcgtgcttt cattctaaac aacattgagg
    40981 tatcagaatc tcatttatgc gaagatactc ttgaagatta cttcaagaaa gtaacagggg
    41041 gtgaaggaat tgcttgatat aatttcatgt gaattttcaa aactcaagcg gttaaaattt
    41101 atactgattt ctattttagg tgcttgctta tttccaattc ccgcaacaat tttgattgca
    41161 aaggacaact tgccctttga gcaattgttc aaattagttg tcaattttgg atattttttg
    41221 ctattaccaa ttgtattaag tattgttgct agccaactat tttttataga aagagataac
    41281 gatgtattaa aaaatcttgc aacagtgcca gtgccaaaag gaaaactggc tctggcaaaa
    41341 ttggctgtgt tgctgtttat atcattgttc tactccgttg cagggttagg agctactatc
    41401 attggaggtt tcattgttgg aatagtagag ggggttactg taaaattagg aatgagcatt
    41461 gcttttggaa ttatgttttt tgttactgta cttcctgttg tggtactcat tgtatatttc
    41521 aatcgcagct atattttttc tattatactt tcttttgtat atgctatttt caattttagt
    41581 atatccttaa atattatcaa ttttgaacca aataacccac tattaagcgt attacctgct
    41641 cctgttataa tgcgttggtg gatggcattt tggggagatc ccactggcga atacacggca
    41701 cttagacagc catatttgct ctcaactcct gcttgtgtag gtattttatg tttgataacc
    41761 attatagcag tcctgctgat ttgtatgata tacaaaaagc aggagaatta ggaggtatct
    41821 atgcttgata taattactac agaatttcaa aaaataaaaa gatataatat tctatggatt
    41881 ggtgttgttg ctgttctgtt ttcagcacta ttggcagttt ttcaggttgt aagttcacac
    41941 ggcagtgacc ctctaacgta tgagagcttt gcaaacggag ttatctggaa taatttcagt
    42001 cttagttttc catttgggat tacacttatt ggaggatatc ttattaaccg tgaatataca
    42061 gaccaaacct taaaaaacat cctaactgtt cctatttcat tacgcaaatt gctagtggga
    42121 aaattaattg cggttggagg aattgctgta ttgttaagtc ttttcagctt cttatgcaca
    42181 tttattttgg ggttgttatt ttgcaatatc aatatatctg ttacactcat tgtaaaatcc
    42241 ttagttcaga ttttgtctgt taatctgtat tgttatgttg ctgttttgcc gattattgct
    42301 tattttggtc gtaaacaaaa tgcatttctg acgggtgtag ggattgcatt tgtatacggg
    42361 ttttgtggtg tgtttgttgc tggaagaaat ctgacagact tttatccaat cacagcaggt
    42421 cttggtctag ttggatacaa caatggtgct ggtactgttt atcagccact tatcggtgct
    42481 atgactttga ttgtaattct aatcttgaca actattctac ttgtatttac acctaattat
    42541 gataaagtta tgaccatatc taagaaaaaa gggataaagc ataaaaagta ttctcataaa
    42601 taacataaat attgtttcta ttatttaagt tgctagacag tgaaaagaaa aaatctgtcg
    42661 tacagtggct atatgctcat gtattcaaga agctacagtt ttgatttttc ataactgtag
    42721 cttctttttg tatttattaa atcacggcta tcataatact tgcaataggt atagcgacaa
    42781 gagagagtac tgtcatgtgt gttttatata ttacattaag tatattctaa aatatatgtt
    42841 ttaaataata tatttgaaaa taggaatatt taaataaaaa aaataatatt atatatagtt
    42901 gacaaaaatt ggcaaatgat ataatatata aatatagaaa tagtaatcat tactattatt
    42961 aaaaagggag gaattaatta tgaacttaaa aggaactaaa acagaaaaga atttaatggc
    43021 aggatttgca ggagagtcag aagcaagaaa taaatataca tactacgctt caaaagcaag
    43081 aaaagaaggt tataatcaaa tagctgctat atttgaagaa actgctaaca atgaaaagga
    43141 acatgctaaa ttatggttta aacttttaca tgatggtatg ccttcaacag aggaaaattt
    43201 aaaggatgca gcagcaggtg aaaactatga atggactgat atgtatgcaa aatttgctaa
    43261 agaagctaga gaagaaggat ttgacaagat agcttattta tttgaagctg ttggaaaaat
    43321 agagaaagag catgaagaaa gatacttaaa gcttttagaa aacttaaatg aaggaaaaat
    43381 atttaaaaga gatgaagaag ttgtttggca atgtcaaaac tgtggtcatg tgtatgtagg
    43441 tactgaagca ccagaaaaat gcccagtatg tgaccatcca aaagcgtatt tcaatataaa
    43501 agctgaaaat tattaaaaat tatcattatt aaaaaggaat gttaactatg aaattttcta
    43561 aacaacgaga actgatttta aatgaaatat taaataatcc ggttcatcct actgcggatt
    43621 acctatatga aaacttaaaa aaagataatc caaatttaag cttaggaact gtttatagaa
    43681 atttagctca gctaacggaa catggcttta taagaaaggt tagtattcca gggtatccag
    43741 atagatttga tggtcgaata gataatcact atcatatcat atgtgaagta tgtggagagg
    43801 tatacgattt agaatccgaa gttcttaata atttacaaga attaatatct gaggagacag
    43861 acataaaaat aacatcttat aacataagtt ttaaagggat ttgtaataat tgtaaaaggt
    43921 gtagccaagt aggttagttt tcaaattcta aaaataatta atgaggtgag tttatgtgta
    43981 gtgaacaaaa attttttata tgtgagactt gtggaaacct tgtaggaatg atacaaagtg
    44041 gtggtgtacc tattttctgt tgtggaaaac ctatgaagga acttgttcct aatacaacag
    44101 atgcagcagt tgaaaaacat gtacctgtaa ttgaagtgga tggaaataat gttactgtta
    44161 aagtaagtag cacaactcat ccaatgacta aagaacatca tatagcatgg gtgtatctta
    44221 tgactgaaca aggtagtcaa cgcaaatgtc tagcagtaga tggagaacct gtagttaaat
    44281 ttgctttgaa tgatgatgat aaagtgattt ctgcctatgc ttactgtaat ttacatggat
    44341 tatggaaggc tgaactgtaa tatttaaata aattgagtca aagaggaggc tataaatgta
    44401 ttttcataca aatagcctcc tataaattta tataaattca agttttaatt ttgtttaatg
    44461 tcaatataat ataaggagaa aataatatgt caatttataa atgtagtgtt tgtggatata
    44521 tttatgatga atctaagaat gataaaacat gggatgaatt aagtgaagat tgggaatgcc
    44581 ctgtatgtac aaaaggtcgt agttattttg gaaagataag cactgtttac tatgaagaag
    44641 atgaaaagat agcagaagac atagttgaaa atgaatctaa attaaatact gaaaaagagg
    44701 gagatttaaa ttacttaagt acatatttaa gaagagatga tgaagttgag aaacatatgg
    44761 atataattca cgaaatggct gttacaggaa aatctataat agaacctatg agaactaaac
    44821 taccagtaat ttcttgggat gatattttaa ttatgggtgc tcaattaaat ccattacctt
    44881 taaatgagca tgatgaagta aatacaacaa caataatagg aaaaaaagca aagaaaccta
    44941 tgattataga aaatccagtt tatatttccc acatgtcatt tggagctttg tctaaagaac
    45001 taaaaatagc tttagcaaag ggtgcagccc aaaataagac agctatgtgt agtggtgaag
    45061 gaggtatatt accagaagag aaggaagctt catacaagta tatttttgag tatgtaccaa
    45121 ataaatatag tgtaactgaa gaaaatctta aaaattctga tgctatagag ataaaaattg
    45181 gtcaaggaac aaaacctggt atgggaggtc atcttcctgg cgaaaaagta actgaagaaa
    45241 tagcaaaagt aagaaataag cctgtaggtc aagatgtaat aagtccatct tgttttgagg
    45301 aaatacaatc caaagaggat ttaaagaaac ttatagatga acttagagaa gtttcagaag
    45361 ggcgtccaat aggtgttaaa atttcagcag gtcatattga aaaagatatg gaatttatag
    45421 cttatgctaa acctgatttt gtaactattg atggtcgtgg tggagctact ggagcaagtc
    45481 caaagttgct taaagatgca acttcaattc caacaatatt tgctctttat cgtgctagaa
    45541 aatacataga tacacatgga ttagatatag atttggttat aacaggtggg cttcgtatat
    45601 ctactgattt tgctaaggca attgctatgg gggctgatgc agttgctatt gcaagttctg
    45661 ctcttatggc agcggcgtgt caacaatatc gtatatgtgg ttctggtaag tgtcctgtag
    45721 gagtagctac tcaggatgag gaattgagaa aaagattaca tatagaaaat agtgctaata
    45781 gagtggcaaa ctttttaaat gtaagtttag aagaacttaa gacttttgct agaatttctg
    45841 gtcataaaga tatacatgat ttaagtgttg atgatttata tactgttaac tctgaaatat
    45901 caaattatac aaacatacag catgtataga ttgaagtttg aaactattag attttagata
    45961 gctataaaag taaatgataa atatatttta taaaagagag tgtcttaaaa tgaacttttt
    46021 agttcataag tcactctttt ttgtatgaag ccaaatatat ttatcatttt agcaatgaaa
    46081 aagaatctta tctctttatt ttgagataga ctccttctat ttatgaaaat taaagatatt
    46141 aaattgtaaa atattctgtg atgtcgtact attaaactaa agattctgta ctttaaagta
    46201 aataaaaatt taaggagtgc ttattatgga aagattgaag atacaagttt tagttgacaa
    46261 taatacttat attgatagat actttgttgg agaaccagca gtaagctatt atattgagat
    46321 tgatggaaat aggattttgt ttgattcggg atattcagat gtattcatat caaacgctga
    46381 aaaattaaat atagatttag gaaatttaac tcatgttgtt ttttctcatg gacataatga
    46441 ccatacaaga ggtatccagt ttttagaaaa taggtatgat ttatctacag ttgaacttat
    46501 ttcacatcct aactgtttta tacctagaaa acatggagag aaaagcatag gagctccatt
    46561 ttctgctgag gaaattaaaa atatttttat gtataatcct aaggataagc catttaatct
    46621 aagtaagaat tgtgtgtttt taggtgaaat accttctata aataattttg aaaaaagagc
    46681 taaaattggt aaatgtaaaa taggagattt gtgggaagat gattatgttt tagatgattc
    46741 tgctatagtt tgtaaaactg ataaaggtat ttttattgtt acaggatgtt ctcatagtgg
    46801 aatttgtaat atagtagagt atgctaaaag ggtatgtgga gatgatagag ttattggtat
    46861 actaggtggt tttcatcttt ttgaattgaa taataggtta gatagtacaa tacaatattt
    46921 ggataaagaa gaaataagaa tgttgtaccc ttgtcactgt gtttctttta aggtaaaagc
    46981 taagatgaat gaaatattga atataaatga agttggagta gggctgacca taagtatata
    47041 gtatttgaaa gtatataaga tttaagttga aggttaaaaa gatattttct atataaagta
    47101 tcttttttgt tacgtttatt ttaagagaaa tcaatatact tttatatgaa atattgtaaa
    47161 aaaatttatt tttaatgaga atatattttc attaataaac aattttatta tgtattaata
    47221 atgctagaat aaatataaat aaatcaaagt tacttacatt aaagtatatt ttttcacaaa
    47281 atatattgat ttattctttt aaagtttgat aattccaatg aatgtaataa aagaagggat
    47341 tttatactat acaattatta ttataaaatt ataaagaaaa aattaaataa taataaagaa
    47401 aaaatgtata atatttaaca ttaaaaaaga tatttcataa aattaaaaaa caaagtgaat
    47461 ttgaatacta tttaaaaaat aaaaaaatat ataaaaaata tttaatacaa aggtagaaat
    47521 aagttgaaaa tgtaaagtca ttggaaaaat atagttgaat ttaatgaaca aaaatgttat
    47581 tgttttatca atgaagtgtt aaaataaaaa ctttcaatat cattttaggg aggatgattt
    47641 catgcaaaaa agtgtaaaga aagcaaaagt aacgggatct atgttagcca tttttggtgt
    47701 tgcaagcgtt ctttttagtt cacatgcagg aggaggattt gctacaggga atcaagaaac
    47761 tcagtactat gtgcagtatg gatggacagc accacttatg gcaattttag caatgattat
    47821 acttactgct accatgagag aagttatcat aatgtataat aataacaatt gtaggaatta
    47881 taaggattta ttttgtgagc tttggagacc atatccaaaa cttgaaataa tatgggagat
    47941 atattactat ctaatggttt taatagctgt aagtgctgtt atagcaggtg cagcagcagt
    48001 atttcaaagt ataggagtaa attattttgt agctgtattt attattggag ttgtacttct
    48061 tgtctttact atatttggtg ctatgcttgt atctaaagca gctacagcaa tgacaatagc
    48121 tattctagta tgtactctta ctatttttat tgttggtata aaagctaaag taccagaaat
    48181 aactgaaata ttatcaaata gaacgagttt cacacctggc tatatcaagc caatacttaa
    48241 tacttttatt tatgcaggtt ttcaatcggt tgttatacct acacttgcag gatgttcaag
    48301 accacttctt aagaactcaa aagaagctac taaggcaatg ataatttcat ttgttatgaa
    48361 tgcaattgca ttaggacttg ctgtaactat gctaatggga tggtatagag aaattattgc
    48421 tgctgggcaa acaacactcc ctactcttta tgttgcaggt caatctggaa atcatactat
    48481 atacataatt tataatgttg cattattcct ttgtttaatg tctacaggag taactactat
    48541 atttggtctt gtaaatagat ttgaagacca taaagcactt tctttccttt cttcaagaat
    48601 gaaaagaagg gtatttacag cttgtgcaat aatggtagtg tcaatgctta tatcacttac
    48661 aggattaagt aatattgtta aatatggata tggatattgt ggttatcttg gtctattcac
    48721 tatagtaatt ccattcctta cactaggaca ttataagaat aagaagtttg caaaggaaaa
    48781 tccagaagct aaatggccag cggaattaaa tgaaaatgta aattagatgt agtagcaaaa
    48841 aatattgcta aagagaatac tataaataag aatactatag ataatatatc tgcatgttta
    48901 ttaagattat ttgcaaaatc taatattgaa caactataaa caagcaatat aaaactaaga
    48961 ataaaagata aaaagagggt gtctcaaaat ggacttttag tttataaggc actctttttt
    49021 atatgaagtc aaatatattt tcattatttt gacaataaaa gagactaatc ctactttgga
    49081 tagcctcttt tttctttaat ataaaatagt ataacagcaa agtatagatt gatacatgtt
    49141 ttctgtagct ttctatatat tacagtgttt gtgtggcatt ggtattttaa gcaatctata
    49201 aattataatt ctataattct tctactaatt ttagattcta tagccgtctc tatttcactt
    49261 aacattcgtt catttttagg gtcaataaaa gcacatgtat agccttcttc accagctcta
    49321 ccagttctac caatacggtg gatgtagtct tctggtgttt ctggtaaatc gtaattatat
    49381 atatgactta ctccagatat atcaaggcca cgagaagcta catctgtagc tattaagtat
    49441 tgtatatcta aatttctaaa tgatttcatt attctttctc gttttgattg agcaatatca
    49501 ctatgaagtt tttgacaatt atatcctctt atagcaagag cttcttcaag attgtcaacc
    49561 cttctcttag tcctacaaaa tattattgcc ataaaaggat tatcttcatc tagtacctta
    49621 cataaggcat ctaatttttt tctatctgta gtttctacta cttcttgttt tatattatta
    49681 agagtgattt cttctttttg aatagaaact acaacaggat tttttgtgta tctataagct
    49741 aattttttta cttgagaatt tatagttgca gaaaagcata gtgtttgctt tttgtttgaa
    49801 gtctctttta aaatagcttc cacttcgttt ttaaatccca taagcaacat ttggtctgct
    49861 tcatctaaaa caaaagtctt taacttacta aggtctattg tttttctatt tagatggtct
    49921 aaaagcctac ctggtgtagc tataatcata tgaatatttc cttttagttt ctttatttga
    49981 gaaccaatat cttttcctcc atatgctgca agaatactta catctttaga ttctttaagt
    50041 ttcatagctt cttctgttat ttgaatagct agttctcttg taggagaaag tataagtact
    50101 tgtatatcat ttatatctaa tgatatattt tcaaatatcg gaagtaaaaa agctagtgtt
    50161 tttcctgttc cagtttgagc ttctgctatt acatcttttc cttcttttat tagtttaata
    50221 ctttcttttt gtataggtgt aggtgttgtt attcctgatt tttttagaat atttattgta
    50281 ttttcactaa tccctaagct tttgaaatcc attgttatct tacctttcat gaaaaatttt
    50341 cttttttatt aaccaattaa taatatcata tttgtaagta tttttagtaa attttttaat
    50401 tattatttat atagcttaaa aatatattaa gatactttaa aatggaatgt aacttaactt
    50461 aaattttagt aggaatttta taggtttatt tttaaaggtt ttttgaaaat aaatctattt
    50521 aaaaattaaa ttaaaatgca agaaaaacat aataactttc atttttaaga agttttcaca
    50581 aaaaagaaaa aatatagtgc aataaatgat acaaagtaat ataatagatt acaagatatg
    50641 aaaagctaaa aaaataaatt gcaaatgaaa ttaaaatgaa taaaaaataa aaaaataatc
    50701 ataagttaga ggtatcaatg aaaaataaga cttaatgttt ttaaaattaa aagtatacta
    50761 aggggggcat taatatgtgc gttatatcaa aagatagagc aaaggaaata aaaatagtag
    50821 atactacttt aagagatgga gagcaaacag ctggggttgt atttgcaaat agagaaaaaa
    50881 taatgattgc tgaaatgtta agtgacctag gtgttgacca aattgaagtt ggtataccta
    50941 ctatgggtgg agatgaaaaa aatgtaataa agcacatatg tagtagaaat ttaaaatcag
    51001 atataatggc ttggaataga gcagtaataa aagatgttga agaatctatt tcttgtggtg
    51061 ttgatgcagt agctatatca atatcagtat ctgacattca tatagaaaat aaattgagaa
    51121 cttctagagg ctgggtttta gaaaatatgg ctaaaacagt tgaatttgca aagaaaaatg
    51181 gattatatgt ttcagtaaat ggagaagatg cttcaagagc agatatagac ttcttaactg
    51241 aatttataaa tgttggaaaa caagcagggg cagatagatt tagatactgt gatacagtag
    51301 gagttatgaa tcctttttct ataaagaatg caatagaaac tctttatgaa agaactaatt
    51361 ttgatataga aatgcataca cataatgact ttgggatggc aactgcaaat gcacttgcag
    51421 gaatagcagc aggagcaaat tatgttggtg ttacagtaaa tggattaggt gaaagagcag
    51481 gaaatgcagc attagaagaa gttttaatgg cacttaagtg tgtttataaa tgtgatttaa
    51541 ataatataga tactagaaaa tttagaggaa tatgtgagta tgtagcacag gcatcaggaa
    51601 gaatacttcc tacatggaaa ccagtagttg gggataatat gtttatacat gaatctggta
    51661 tacatgcaga tggagcttta aaagacccgc ataactatga accatttgac ccaagtgaag
    51721 taaatcttga aagaaaaata gtaataggta aacattcagg aagagctgca gttataaata
    51781 agttgagtga atatgaaatg tatatttctc cagaaaacgc taccaagcta ttaaatgcta
    51841 taagagctac atctataaga ttaaaaagaa gtttaatgga taaagaaatt cttcaattat
    51901 attgtgatat actagcacat gagaaaggca caacagaaga agaagcagtt agaggttcat
    51961 atatttaaga tataattgtt taggaggaaa atatgggaga taatatagtt tataaaataa
    52021 ttaaaaaaca tatagtagat ggagaagctg ttgctggaag ttcaattggt ataaaaatag
    52081 accaaacttt aacacaagat tctactggaa caatgacata tcttcaacta gaagctatgg
    52141 gaatagataa agtaaagact aaaagaagtg tggcatttgt agaccataat atgttacaac
    52201 aaggatttga aaatgcagac gaccataagt atatacaaac agtggcagat aaatatggag
    52261 tttatttctc taagccagga aatggtattt gtcatcaagt atttttagaa agattttcaa
    52321 caccaggaga tacactttta ggttctgata gccacacacc tactgctgga ggtgtgggca
    52381 tgatggctat tggagctggt ggattagatg ttgctttagc catggcagga ggagcatact
    52441 atataaaagc accaaaagtc tgtaaggtga atctagttgg aaaattaaat aatatggtat
    52501 catcaaaaga tataatatta gaagtgttaa gaaaacaaac tgtaaaaggt ggagtaggta
    52561 aagtttatga atatggtgga gaaggtgtca aaagcctatc tgttccacaa agagctacaa
    52621 taacaaatat gggggctgaa cttggagcaa ctacttcaat cttcccaagt gatgaaaaaa
    52681 cactagaatt ttttaagagt caaggaagag aagacgcatg gatagagtta aaaccagatg
    52741 cagatgcagt ttatgatgaa gagattacta taaacttgga tgaattaaaa ccattagctg
    52801 caaaacctca tagcccagac aatgtagatg aagttgaaaa tataggcaag attaaaatag
    52861 accaagtggc aataggaagt tgtacaaatt cttcttatga agacttaatg aaagtagcac
    52921 aaatcctgaa aggaaataaa gtacataagg atgtgagttt ggtaatagca ccaggttcaa
    52981 gacaagttat ggagatgatt gctagaaatg gggctttggc agatataata agtgctggag
    53041 ctagaatact agaaaattct tgtggtccat gtattggtat gggtcaatct ccaggtactg
    53101 actctgtgtc tttaagaact ttcaatagaa atttctatgg tagaagtggg actttgtcag
    53161 cacaagtata tttagtaagt ccagaagtgg cagcagtaag tgcaatcaag ggagtgctta
    53221 cagacccaag agaatttgat attaaattta caaatttaga tgtaaatgaa tttttgatag
    53281 atgatagcat gataattaaa cctgctgatg taggaagtaa tgtagaagta gttagaggac
    53341 ctaatataaa acctttccct cttaatacag agctaagtca gtctataggt ggaaaagtta
    53401 tattaaagac agaagataat ataactactg accatataat gccatcaaat gctaagttac
    53461 ttccttttag aagtaatata ccgtatcttg caaactattg tttcaatact gtagatacag
    53521 aatttcctca gagagcaaaa gacaataatg gtggatttat agttggtgga gataattatg
    53581 gtcaaggttc aagtagagaa catgcagctt tagcaccatt atatctaggt gttaaagggg
    53641 taatagttaa gagctttgca agaatccaca aggctaactt gataaatagt ggtataattc
    53701 caatggagtt ttgtgatgag aaagattatg aaaatatatc tcttttagat aatctggaga
    53761 taccaaatat tttagacaat ctaggtagtg gaattttaga agttaagaat acaactaaag
    53821 gaacttcttt taaggtcaaa gtagaattgt cagcaaaaga agttgacgta ttaaaagctg
    53881 gtggaaaatt aaactacact aaaaatcaag ctaattaata tgaaaagagg gaatattgaa
    53941 tgtataaagt gacacttata ccaggagatg gaataggacc agaagttgcg aaagctatga
    54001 aaaaggtagt agaagctact ggagcagaaa tagaatggga agaagttaat gcaggagagg
    54061 cagttattga agagtatggt actccacttc ctgaatatat tatagatagt ataaaaaaga
    54121 acaaaatagc tataaaaggc ccaattacta ctcctgtagg taaaggattt aggagtgtaa
    54181 atgtaacttt aagacaggca ttagatttgt atgtcaattt aagacctatt aaaagtttta
    54241 aggggataaa atcaagatat gaagatgttg atttagtggt tgtaagagaa aatacagaag
    54301 atttatatgc tggaatagaa cataaaatag gtgattatgc agctgaaagt ataaagataa
    54361 taacaagaag tgcaagtgaa agaatcgttg attttgcatg taactatgtt aaagataata
    54421 agagaaaaaa agttacagct atacataagg caaatataat gaaaatgtct gatggattgt
    54481 ttttagatgt gtttagagaa gttgcatcaa aacatggtgt agaatatgat gatttgattg
    54541 tagatgcagc agctatgaac ttagttttaa atccagaaaa ttatgatgta atggtaatgc
    54601 caaatcttta tggagatata ctatcagatt taggtgctgg gcttgttgga ggtttaggta
    54661 taattccaag cgcaaatatt ggtaaagatt gtgcgatatt tgaagctgtt catggaagtg
    54721 ctccacaaat agcaggtcaa aataaggcta atccaacagc acttattcaa tcatcagtaa
    54781 tgatgcttag atacttaggt gaatatgaaa atgcacaaaa aatagaaaca gctttagaaa
    54841 aagtatttct tgaaggaagt aaattgactg tagatttagg tggctcagct tctactacag
    54901 agtttgcgga tgaggtgtgt aagtatattg tatagatttt atttagggaa aaagtgaaat
    54961 atagattgaa ggaaagaggc tttctcaaaa tagagataag ccttttttta ttggaatgat
    55021 aaaatatatt tgattttata caaaagagag ttcctcataa acgaaaaagt taattttggg
    55081 atatccattt taattttaaa attaattgat taatacgcaa agaaagatta aacaaattta
    55141 ttatttttaa ttgaactttc aaccaaaata tggtaaaatt taaaaaagtt gaactttcaa
    55201 ctaaaaataa atatattgat tatgtgaaat tctataaaac agaaactatt tgactttagt
    55261 catggtaggt tcagtggagg tgtgtttgat taagacttta gatagtaata tattaagaga
    55321 agtaggtact ttatctagag cagtaaattc aataaatgat attaagtaca aggaattaaa
    55381 gttacaaaaa ggacaattta catttttgac aagaatctgt gaaaatcctg gtataaatct
    55441 tgtggaatta tcaaatatgt taaaggtaga caaggctaca acaaccaagg ctatacaaaa
    55501 actaataaag gcaggatatg tagataaaaa gcaggatgag tttgataaaa gaggatataa
    55561 tttaactcca actgataaat ctttggaagt ttatgagttg ataattgaag aagagaatag
    55621 aagtatagag atatgttttg ataattttac agatgaagaa aaacaagtgg taactaagtt
    55681 attagaaaaa atgagcaaaa acgtagaaaa tgaatggttt aaagtaaaga gatagtaaat
    55741 aaatacatta acttaaggag atatttttat gattagaaaa gcgaatatga atgatttaga
    55801 aagcattatg aaaattataa aatctacagt ggaagaaatg aagacttata ataatacaca
    55861 atgggatgaa aactatccat tagaaaaaga ctttgtttca gatattaaga aacaagattt
    55921 atatatttat gaagtggatg gagaagtagc aggatttata tgtcttaatt atgaagagcc
    55981 agaagaatat ttaggcttaa attggtcatc taataaaaaa gcaatggtaa tacatagaat
    56041 ggctgtaaat ccaaacttta gaaagacagg aatagcatct aagttagttg attttgctga
    56101 aaaattagct gtagaaaaca atgtatcata cttaaagagc gatacatatt caataaactc
    56161 aaaaatgaat ttattactta caaagtgtgg ttttataaaa atcggagaaa tgagtttttt
    56221 aggaaaggag aaatctttct actgttatga taaaatatta taaagagcat ttataataca
    56281 aggggtaaat aatagatttt cagcattagt aagggcataa tataacccag tatatgctat
    56341 tatgaaaggg atgatttata tgattggatt tctaaagaaa agaagaagta ttagaaaata
    56401 caaggatgtt gaagtagaaa aagaaaaatt agataaaata ttaaaagctg ctcttttagc
    56461 tccatcatca aaggggctta gaacatggga atttatagtg gtagatgata aagaaaagct
    56521 aattaattta tctcaatgta gaactaaggg tggtggattt ttcttaaaaa atgcaccttt
    56581 agctattgta attattgcag ataaagaaaa aaatgatgtt tggatagagg atgcaagtat
    56641 agctgcttca tacatacaac ttcaagctca tgaactaggt ttaggttctt gttggataca
    56701 agtaagaaat agaatgtatg atgataatat agaagctgat aagtacatta gagaagaatt
    56761 aaaagtacct agcaagtaca gtgtagaatg tataatttct ataggatatt ctgatgaaga
    56821 aaagaaagca tacaatgact ctgatttaga ctataaaaag gttcatttta acaactttta
    56881 gtatataata tgttgtatta agacattata tttttttcta ttgttaatat atacttaaaa
    56941 ttgtaaaaat atatctgaat tttactaact tatctagggg ttatgtacaa ggattttaaa
    57001 gataaatata gaaattaatg tttataatcg atacataact tgagtaacaa aatttaagga
    57061 ggttaaagta tgggtatatt taaaacaaaa atggatgagg actggaaagt aaactacatt
    57121 aaagagttta atgaaatgag agattcttat gagagtaaac ttcaaaaaaa acagtttgag
    57181 gtagatagtt tgaaatcaga attggataga ctaagaagct ataaaaatag ccttaagcca
    57241 aaagaaaaac aaattactga tgatgatata aataatataa agagtttaag aagagatggc
    57301 ttaagctata aagaaatttc aaatcaaaca agttggagca aagcaactgt aagtagggta
    57361 ttaaatggac tttacgatta gcatatacat ggggtgattt aaattgggat gaagtatagt
    57421 tacaaaaaat gcataataga ttctatttta ttgatgttta taattcaaat attaaggatg
    57481 attttgaatt atgtacttct aagtcaattt gaatttactc ttgaaaattt taatattata
    57541 aacttaattt cttttacatt agttggttta tcgttaattt tatttttaaa agataattct
    57601 ttgtataaca aggttagaaa tagaaaaatc acagaagctt ttgaagaaaa taaaaacaat
    57661 atacttattg aaaagtgtaa gcttatactt tttgttgtgg ttttatcttt agcaataatt
    57721 gtaacttatt gtactaaggg atatgtactt tttaatgtaa ctatgatgac tttgtctgta
    57781 ttgatagttc caatatttga agagttattt tttagagaat atatatggaa ctatctaagt
    57841 aattttataa agtcaaagag taaaattatc tgtataacat ctatactatc tggaatttat
    57901 aatataggat acatagatgt tataagaaat tatgtaatat tgtataacaa ttcaagctac
    57961 acctttgaag ttattatttc aaaaattatg ataggtactg tttttggtat tgtacttgga
    58021 ttagttaagt ataggtttag agatgtaggg ttttgtatat tattgaggag cttatttgct
    58081 atatttatta ggtagatagt ataatcggtt tataaggttg ttttaaattt aattttaaat
    58141 caaccttatt ttatttgtg
//
LOCUS       DAELXX010000009        57188 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.009, whole genome shotgun sequence.
ACCESSION   DAELXX010000009 DAELXX010000000
VERSION     DAELXX010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 57188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57188
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.009"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..569)
                     /locus_tag="KPH29_000557"
     CDS             complement(<1..569)
                     /locus_tag="KPH29_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG1466197.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIG"
     gene            738..2096
                     /locus_tag="KPH29_000558"
     CDS             738..2096
                     /locus_tag="KPH29_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466198.1"
                     /translation="MSNIITNLIESVFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKVRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FSLIIGYFTKIKDCEHVLIQIMLKSENLLTSEITHFKSERMDLKVEEVKNTIKQISKS
                     IEAIEKFDKISKDLNDFNDEFISGIEALKSLLEGSQSSIKTFDQSVRKLDKQFNILNI
                     KFVKLFDKYDNQDNINKEILFDIKESSKNIYNATESQFKIRDYIKNINAGFALYERSA
                     QDLLTKLMTHENKISQNQKILLDEKFTLDDSIKNLSSIIENFSNDLQVKLDMMFENSL
                     DIQDKLDVMFNNSFMNDEVPLDSEELFKDDVNNVFNPFSEEIYEIEDKEIKVIGEDEL
                     NE"
     gene            2089..2721
                     /locus_tag="KPH29_000559"
     CDS             2089..2721
                     /locus_tag="KPH29_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBG1466199.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSKIPVKVDKSNGQVTFGEKTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
     gene            3050..4207
                     /locus_tag="KPH29_000560"
     CDS             3050..4207
                     /locus_tag="KPH29_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG1466200.1"
                     /translation="MIEISIKNKKNSKIKTLILLLIVSISIYLTGCTTSDNGNNQSNN
                     NDSLSSSISSDKNVKIHFIDTGNSDAILIQDGKTFTLIDGGDNDDEGLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILP
                     KLEKVDLLKVAHHGSRSSSSQEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNT
                     FNSEEIVYFTPKGKSYHSIKNCSGLSRSKKILSGTIEESKKNDPCDICYGK"
     gene            complement(4355..4990)
                     /gene="lexA"
                     /locus_tag="KPH29_000561"
     CDS             complement(4355..4990)
                     /gene="lexA"
                     /locus_tag="KPH29_000561"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBG1466201.1"
                     /translation="MYLDLTEKQVLILEFIKSQITLKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(5094..6221)
                     /locus_tag="KPH29_000562"
     CDS             complement(5094..6221)
                     /locus_tag="KPH29_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG1466202.1"
                     /translation="MNNDKKIIKVHNKSDKPDNIVIKENELIEKCLLLENKLPVFMKD
                     YFIYLKGSVAVSTRLAYLEDINFFCSYLIETKELTNADCVKDITEDDFNTIKSRDINL
                     FLGDYCSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPI
                     KLPKPQPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGL
                     RLNELRELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLND
                     EEKDALFLSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHKLRATAATSLIQTGF
                     SIYDVQNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
     gene            complement(6543..7388)
                     /locus_tag="KPH29_000563"
     CDS             complement(6543..7388)
                     /locus_tag="KPH29_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466203.1"
                     /translation="MIYIIEILKISVINIFCFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTIMIILIFKILLPDSYMKVTQNIDLKLYVSMINNLNING
                     FIHIILGDISNFIRIMLLTPSVYSLKYFIFMFLMYSISTHMSLSSADLKNSLNGLSFI
                     VIVIFVISSITYLFGFDNLNISSLIVKYNIFISFFMAIGFIFSIVTLIISYILSLVSP
                     FKNFS"
     gene            complement(7418..7789)
                     /locus_tag="KPH29_000564"
     CDS             complement(7418..7789)
                     /locus_tag="KPH29_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBG1466204.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIEDACKILSCNGYEVE"
     gene            complement(7942..8202)
                     /locus_tag="KPH29_000565"
     CDS             complement(7942..8202)
                     /locus_tag="KPH29_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HBG1466205.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            complement(8391..9335)
                     /locus_tag="KPH29_000566"
     CDS             complement(8391..9335)
                     /locus_tag="KPH29_000566"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HBG1466206.1"
                     /translation="MIENNSEKIKVLENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSMVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            complement(9356..10210)
                     /locus_tag="KPH29_000567"
     CDS             complement(9356..10210)
                     /locus_tag="KPH29_000567"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBG1466207.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKSLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKELLY
                     QILAMHKK"
     gene            complement(10225..11586)
                     /gene="accC"
                     /locus_tag="KPH29_000568"
     CDS             complement(10225..11586)
                     /gene="accC"
                     /locus_tag="KPH29_000568"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBG1466208.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            complement(11602..12054)
                     /gene="accB"
                     /locus_tag="KPH29_000569"
     CDS             complement(11602..12054)
                     /gene="accB"
                     /locus_tag="KPH29_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBG1466209.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            complement(12324..12770)
                     /locus_tag="KPH29_000570"
     CDS             complement(12324..12770)
                     /locus_tag="KPH29_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466210.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVVFGF
                     IGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKENDTNLKESEV"
     gene            13045..13323
                     /locus_tag="KPH29_000571"
     CDS             13045..13323
                     /locus_tag="KPH29_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466211.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     VLVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            complement(13452..14198)
                     /locus_tag="KPH29_000572"
     CDS             complement(13452..14198)
                     /locus_tag="KPH29_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466212.1"
                     /translation="MWEGTMNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDAN
                     LRAVVKRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEV
                     PKEALSLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHN
                     VDIAKGESIDLRKIITKKIDSYYKYWEVTDNLPKDLDFIVSVSDKSVLSCEDMIIKGN
                     KTGQAVVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(14327..14971)
                     /gene="trmB"
                     /locus_tag="KPH29_000573"
     CDS             complement(14327..14971)
                     /gene="trmB"
                     /locus_tag="KPH29_000573"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBG1466213.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            complement(15109..15429)
                     /locus_tag="KPH29_000574"
     CDS             complement(15109..15429)
                     /locus_tag="KPH29_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HBG1466214.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
     gene            complement(15882..16307)
                     /locus_tag="KPH29_000575"
     CDS             complement(15882..16307)
                     /locus_tag="KPH29_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG1466215.1"
                     /translation="MHDELKIKMYSFTMDCKDPHELAKFYAALLNWEIAFYNEEYACV
                     GAPGTNQGTYPGITFQRNSEYKPPVWPEKPESQQQMAHMDFAVNDLEEAVQYEIHCGA
                     TIAEEQFTDDWRVMIDPAGHPFCLCQMKSIMESSHFSLL"
     gene            complement(16460..16921)
                     /locus_tag="KPH29_000576"
     CDS             complement(16460..16921)
                     /locus_tag="KPH29_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBG1466216.1"
                     /translation="MKFGGVLLIVKDINKSKKFYEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRLLKQGLALEEISKRTMYPVEFITQFQQL"
     gene            complement(16959..17792)
                     /locus_tag="KPH29_000577"
     CDS             complement(16959..17792)
                     /locus_tag="KPH29_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1466217.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCMQLKNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSLMYICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
     gene            complement(18065..18751)
                     /locus_tag="KPH29_000578"
     CDS             complement(18065..18751)
                     /locus_tag="KPH29_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1466218.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKEVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALVGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TITVSDGLIV"
     gene            complement(18766..21243)
                     /locus_tag="KPH29_000579"
     CDS             complement(18766..21243)
                     /locus_tag="KPH29_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG1466219.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMYGTATISVQSKNPDVLTNLEDIPDLNAIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRVLIESEEEMTENL
                     VNKLSEELSTEKYMVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGKMNEEFIENVRHMDGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIEAFNDKRSVILNEDTLKKVGLSIGDTITLSNDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDVVMIQIRDLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKKRSIAMYKSIGLSDK
                     QNIKVTLIEGFTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            complement(21463..22866)
                     /locus_tag="KPH29_000580"
     CDS             complement(21463..22866)
                     /locus_tag="KPH29_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1466220.1"
                     /translation="MHKPSYKTVFHSYIIFFMIFICLILTVIGIFLSLITIQKPNGEF
                     IRSDWPKKFTENFNEQIIFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLTLANQNNSSNGTVYFNTIKNNKNDYIYIIYFPLNISKVNMFFDGSK
                     FVGGKSIVFSIIGIIFLIILISGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVF
                     ADIYNSLNILNEEIHRSDEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSST
                     NYSPEKVYKYASIMLKNIEYTNTLINDLKLTYQLKNGIIPLNKNKYDLVRFLKELSID
                     ILNYPEYESNIVEFKSNIDNINFDFDCTLLKRAFSNLIVNAFTYGDKNTKVCVHITLN
                     ERIEIIISDNGKGMSREEASNLFNRYYRGSNTKQKIEGTGLGLAITKQIIEVHGGNIS
                     VESIPGLGSSFYITFPI"
     gene            23365..24225
                     /locus_tag="KPH29_000581"
     CDS             23365..24225
                     /locus_tag="KPH29_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG1466221.1"
                     /translation="MKKQKSPILLEMLGLFLGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
     gene            complement(24465..25022)
                     /locus_tag="KPH29_000582"
     CDS             complement(24465..25022)
                     /locus_tag="KPH29_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG1466222.1"
                     /translation="MSDSEYPQMKTLETRSCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSTKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
     gene            complement(25212..26099)
                     /locus_tag="KPH29_000583"
     CDS             complement(25212..26099)
                     /locus_tag="KPH29_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG1466223.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENEKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFLSMISNNI"
     gene            26218..27417
                     /locus_tag="KPH29_000584"
     CDS             26218..27417
                     /locus_tag="KPH29_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HBG1466224.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            complement(27611..30166)
                     /locus_tag="KPH29_000585"
     CDS             complement(27611..30166)
                     /locus_tag="KPH29_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG1466225.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKKNKKSKEYIVDRLGVTKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETAMEGFVNDYLQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWPLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIGVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKRGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTGEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINTPLYIGMIILNITISIVAVIIPVRQILKSDIISEINKS"
     gene            complement(30163..30849)
                     /locus_tag="KPH29_000586"
     CDS             complement(30163..30849)
                     /locus_tag="KPH29_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1466226.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGEVL"
     gene            complement(30926..31834)
                     /locus_tag="KPH29_000587"
     CDS             complement(30926..31834)
                     /locus_tag="KPH29_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1466227.1"
                     /translation="MFILAFIICTVVIFIVSTKINQRRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKSEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            complement(31836..32507)
                     /locus_tag="KPH29_000588"
     CDS             complement(31836..32507)
                     /locus_tag="KPH29_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1466228.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKACLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            32769..33833
                     /locus_tag="KPH29_000589"
     CDS             32769..33833
                     /locus_tag="KPH29_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HBG1466229.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
     gene            33991..34677
                     /locus_tag="KPH29_000590"
     CDS             33991..34677
                     /locus_tag="KPH29_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1466230.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKENYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINLFKQKVKAFLNHLTTKTKQDYYRDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKSILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLKYIKTVYGMG
                     YMWLGERNEF"
     gene            34667..35698
                     /locus_tag="KPH29_000591"
     CDS             34667..35698
                     /locus_tag="KPH29_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1466231.1"
                     /translation="MNFKKFSFSSIYLIIAVGIIFTAILFNISIYLITDDLKLIALLL
                     IYTIVLCGFIALIIFLLHKKIVIFCSKICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LIRMYEVIQENRNSIAIEKADLQELVSDLSHQIKTPIANLKMINTTLLNQTFKTEIQK
                     DFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTNSNGKIRVATECWVMYTKIDII
                     DNGKGISESHQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIGKGS
                     TFSIFLPNK"
     gene            35780..36445
                     /locus_tag="KPH29_000592"
     CDS             35780..36445
                     /locus_tag="KPH29_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1466232.1"
                     /translation="MSILQVINLKKYYGQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMTDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDMPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGSLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            36458..38875
                     /locus_tag="KPH29_000593"
     CDS             36458..38875
                     /locus_tag="KPH29_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1466233.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKDKNLSGVGTYKLIGSNKKTDCIMSIV
                     YADDTAIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLKKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNSVQMYAKLKSLGMTSFQLKKTISLQGNILSIIFIPLGAI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKQAKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKSIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     SYGLNLKVGDTVTFNIFSGNDIIKKKMKIIGIIDNSNTGGMFFASDKTLESLTPYNTN
                     LDFSILIDKKHSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSSI
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLIRNIEKKSL
                     TERLRSQ"
     gene            complement(39056..39847)
                     /locus_tag="KPH29_000594"
     CDS             complement(39056..39847)
                     /locus_tag="KPH29_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG1466234.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(39880..40467)
                     /locus_tag="KPH29_000595"
     CDS             complement(39880..40467)
                     /locus_tag="KPH29_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG1466235.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYRNSKSVSPYLLLPLSYIEFAQEETYLFKL
                     LFINDLDLMMKEANDFYKEIDNEKKARLFSESIGIELECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKPDWNLSI"
     gene            complement(40644..42035)
                     /locus_tag="KPH29_000596"
     CDS             complement(40644..42035)
                     /locus_tag="KPH29_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HBG1466236.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSKEIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            complement(42225..43523)
                     /locus_tag="KPH29_000597"
     CDS             complement(42225..43523)
                     /locus_tag="KPH29_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/BAAT N-terminal
                     domain-containing protein"
                     /protein_id="HBG1466237.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSTERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKGIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLEALEIFEENNYPYNVELLTYNNMGHPLPIPYIMPLKETLCMS
                     MSGGIFSSGGTVEGNSKGQFESWNRTIEFYKKLLSSDLNN"
     gene            complement(43884..44759)
                     /locus_tag="KPH29_000598"
     CDS             complement(43884..44759)
                     /locus_tag="KPH29_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HBG1466238.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLN
                     SFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDIDTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITA
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIKEYKASLMDIYINELMESDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFANA"
     gene            complement(44952..45638)
                     /locus_tag="KPH29_000599"
     CDS             complement(44952..45638)
                     /locus_tag="KPH29_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBG1466239.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(45687..46220)
                     /locus_tag="KPH29_000600"
     CDS             complement(45687..46220)
                     /locus_tag="KPH29_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBG1466240.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDA"
     gene            complement(46264..46896)
                     /locus_tag="KPH29_000601"
     CDS             complement(46264..46896)
                     /locus_tag="KPH29_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HBG1466241.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYNTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(46986..48065)
                     /locus_tag="KPH29_000602"
     CDS             complement(46986..48065)
                     /locus_tag="KPH29_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBG1466242.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSNVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            complement(48198..49544)
                     /gene="pepV"
                     /locus_tag="KPH29_000603"
     CDS             complement(48198..49544)
                     /gene="pepV"
                     /locus_tag="KPH29_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBG1466243.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELDKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(49613..50893)
                     /locus_tag="KPH29_000604"
     CDS             complement(49613..50893)
                     /locus_tag="KPH29_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HBG1466244.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(51020..51280)
                     /gene="hfq"
                     /locus_tag="KPH29_000605"
     CDS             complement(51020..51280)
                     /gene="hfq"
                     /locus_tag="KPH29_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBG1466245.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            complement(51394..52329)
                     /gene="miaA"
                     /locus_tag="KPH29_000606"
     CDS             complement(51394..52329)
                     /gene="miaA"
                     /locus_tag="KPH29_000606"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBG1466246.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDIDAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINEKGKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMIKNG
                     LVEEVKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTARWFDLDQYENIDELKNEIILYIKDSIK"
     gene            complement(52367..54334)
                     /gene="mutL"
                     /locus_tag="KPH29_000607"
     CDS             complement(52367..54334)
                     /gene="mutL"
                     /locus_tag="KPH29_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBG1466247.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKDKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYLCSSKRKFSLYGYSVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNVDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(54345..57188)
                     /gene="mutS"
                     /locus_tag="KPH29_000608"
     CDS             complement(54345..57188)
                     /gene="mutS"
                     /locus_tag="KPH29_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBG1466248.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIEKLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KEYVSEMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKTIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAESADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHELIVKD
                     YKHIKKDYDKLNSKFKALNDEVALMKQNDDKEKINQEDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
BASE COUNT        19655 a         8841 c         6644 g        22048 t
ORIGIN      
        1 ccaatatcat gtgtaaccaa aatagtagac ttattctcat tcttaataat actataaaca
       61 tcatcacaaa ctaataatct agtctgataa tcaagagctg aaaaaggttc atctaaaagt
      121 agaatatctg gatttacaga aagtgttcta ataagcgcaa ctctttgtct catacctcca
      181 gataattctt ttggatacat acttctaaaa tcccataatc cataagtctt taaaaggctt
      241 tcaactctat ctaaagattt tttatctttt ttgccttgaa tttccaatcc aatagttata
      301 ttatccatta tatttctcca ttcaagcaaa ttatcttttt gaaacatata tccaatattt
      361 ccagtaatct ttatatcacc actagtcggt tttaaaagat tagtaagaat atttaatatt
      421 gtagatttac cacttccaga aggaccaagc aaagttaata tttctccttc tttcaagtta
      481 aaattgacat cctttaatac ttcaagctcg ccttccttag tgtagaagtt catatttata
      541 tttttaactt ctactatgtt gtcattcatt atgccacccc ctataacttt tagctcgtat
      601 atattatgtt attaacaata tgacctattt gtaccatttt tcataaatat aatataaata
      661 ttttgtttat ttacgtattt tcttagtaac ttgtacgtat aaattataat aaataaatta
      721 taaggtaggg gataaatttg agtaacataa taacgaattt aatagagtca gtatttacta
      781 atccaataac tatattgttt ttacttgcaa taactgtgta ttctgttttt aaaataatag
      841 aaaataacag agtatattca tttgtaagtt caaaattaga cgaaatcaat agagattata
      901 agacaagtga gttttataaa aaggtaaggg atgactacta tacatatagt aaagaaaatc
      961 cttatgcaga cgtaaatata acttcattta tagaggaagt tgtatctgat ttaaagcata
     1021 ataatttgcc attgttagaa aaaattagaa gtataaaaaa ttcatcttca ataagtatat
     1081 tacttggggt tttaggtacg tttgttggtc tttctacaat gcttttatgt gttgatacta
     1141 aagacattat aaattcactt ccttcaacta ttagtagtat gcaaactgca tttacaacta
     1201 gtatatttgg agttgtattt tctctaatta taggttactt tactaagata aaagattgtg
     1261 aacatgtttt gatacaaata atgttaaaat ctgaaaattt actaacatct gaaataactc
     1321 attttaagtc agaaagaatg gatttaaaag tagaagaggt aaaaaataca ataaaacaaa
     1381 taagtaagtc tatagaagct attgaaaaat ttgataaaat atctaaagat ttaaatgact
     1441 ttaatgatga atttatcagt ggtatagaag ctttaaaaag ccttttagaa ggttctcaaa
     1501 gttctattaa gacatttgac cagagcgtaa gaaaattaga taaacagttt aatattttaa
     1561 atataaaatt tgtaaagctt tttgataaat atgataatca agataatata aataaagaga
     1621 ttttatttga tataaaagaa tcttcaaaaa atatatacaa tgccactgaa agccaattca
     1681 aaattagaga ttatataaaa aatataaatg caggatttgc tctatatgaa agaagtgctc
     1741 aagatttatt aactaaatta atgacccatg aaaataaaat ctctcaaaat caaaaaatcc
     1801 ttttagatga aaagtttacc ttagatgact caataaaaaa tctctcaagt attattgaaa
     1861 acttttctaa tgacctacaa gttaaactgg atatgatgtt tgaaaattct ttagatattc
     1921 aagataagtt agatgttatg tttaataact cttttatgaa tgatgaagtt cctcttgata
     1981 gtgaggaatt atttaaggat gatgttaata atgtatttaa tccttttagt gaagaaattt
     2041 atgagataga ggataaagaa ataaaggtca taggtgagga tgagttaaat gaataataaa
     2101 tatagaagaa ctgttaacag agaatttgaa aagagtagct tttggccaac atttacagat
     2161 ttattatcta ctgtattgat ggttgtgatt cttatcttat ttagctcaga aagtatttca
     2221 ggttctgttg aacaagattt agctaagaat gtcaatgctt ctgttgaaga aacttttaaa
     2281 aaaagcaaaa tacctgtgaa ggtagataaa tctaatgggc aagtaacttt tggtgaaaaa
     2341 accatgtttg atgtagatag tgatgtttta aaaccagagg ctaaagaaat gctaaaaatg
     2401 tttgttccaa agtacataga gactatttat aaagattatg gtgactatat atccaaaata
     2461 gttatagaag gtcatacaga tgatgtggga agttatatat acaacttaga tttatcgcaa
     2521 agaagagcat atagcgttgc taaatttata gtgggagatg aaataggtga ctataaatac
     2581 aaagataaag tgactaagca tattattgca ataggaaggt caaaagctga actcatcaaa
     2641 aatggtgaca atagcgtgaa tagagatgca tctagaagag tagaactaaa atacgaaata
     2701 aatattaatc aaaataaata attaaatttt tataaaaaaa aagaaggcag ttggtgtgag
     2761 ctttaagcct tctttaagga aaaagtttat ttatttttgt tgatacttaa catattatag
     2821 tttttagaag cagtttgcaa tatattaaac gtaaatggtc gtttaaaatg cataactgga
     2881 tactactgat gtaaaatatt tcattttata taatataaaa attatacagt gtcaattttc
     2941 tatattattg tactataatc tattatttaa aatattatgt aaaaaaatat cataaaaacg
     3001 catatcatgt tataattact agagtattat gaatatggag gcgtagtttg tgatagaaat
     3061 aagtataaaa aataaaaaaa attctaaaat taaaactttg atacttttat tgatagtatc
     3121 tatatcgatt tatttaactg gttgtactac atcagataat ggaaataatc aatcaaataa
     3181 taatgattct ttgtctagta gtatatcaag tgataagaat gtaaaaattc attttattga
     3241 cactggaaat agtgatgcaa tattgattca agatggtaaa acttttacat tgatagatgg
     3301 gggagataat gatgatgagg gattaatggt agattaccta aacaatcaag gtgtaaaaga
     3361 tattaaatat cttatagcta cccattctca tgcagaccat ttaggtggac ttgattctgt
     3421 tgtcaaaaac tttaatatag aaaatgtttt tgtatcgaat ggttcagcag aaacaaagag
     3481 ttatagggac tttataaatg ctttagcaaa taagggttta tccccaagtg taccgcttga
     3541 aaataataag ttctatttag aagattctta ttttgaagtt ctaaatacaa atgggggaga
     3601 tactactaat gaacaatctc tagtccttgt atatactaat gggaatgata aagttttatt
     3661 tacaggtgat gctgaagaag gtaccgaaaa agaaatacta cctaagcttg agaaagtaga
     3721 ccttttaaaa gtagctcatc atggttctcg tagtagttca tctcaagagt ttttagataa
     3781 agtaaatcct gaatatgcgg ttcttttagt aggcaagggt aattcatatg gtcatccaca
     3841 tcaggagaca atgaataaat tgaaaaaaat gggagtaaaa gtacatagaa gtgatgagtg
     3901 ttcagatata atatttgaat caacaggtga tggagtattt acaagttgta aagatggaag
     3961 ttataataaa ggtgttagag aagatggaaa ctctggaaaa tcaaacagta atacaagtaa
     4021 aaatgattct tttaaaaata aacaaaatac attcaattca gaagaaatag tgtattttac
     4081 tccaaaaggt aagagttatc actctataaa aaattgtagt gggctttcaa gaagtaaaaa
     4141 aatattatca ggcacaattg aagaaagtaa gaaaaatgac ccatgtgata tatgttatgg
     4201 aaaataacaa aaaaacaacc aatttatcta taaagcttct gaactttaaa tcatttaaaa
     4261 aataaaataa atgcataaaa aaaataagct ataatcaata gataatacat ttcatctata
     4321 cgaatagctt atttttttaa gtatttttta atagctattt aataactcta aatacaccag
     4381 ttacaatacc tacaatttca acttctgagt cattgagcat aattggttcc ataaaatcat
     4441 tttctggctg aagtcttatt aaatttcctt ctttaaaaaa cctcttaact gtagcagatt
     4501 caccatttat aagtgcaact actatctgag aatttaaggc cgtatttttt ttgtctacta
     4561 caacgtaatc ttcatctaaa attcctgcat taatcatact ttcgcctttt actcttaata
     4621 caaaattgtt actgcccttt actaaactgg cagggaatgg aatgtattcc tctatgtttt
     4681 gttgagctaa aatgggttct ccagctgtta tttgacctac taaaggaagc tctattattt
     4741 cttgattagc tccagaaaca tcatttactt tactacgttc taaaacttca atagctcttg
     4801 gtttagtagg gtcctttctt atgtatccca gtttttcaag tttatttaga tgagagtgca
     4861 cagttgaagt agatcttaac cctacagcag tacatatttc tcttacagca ggtggataac
     4921 cttttaatgt gatttgagac tttataaatt ccaatatcaa gacttgcttt tcagttagat
     4981 ctaaatacat aacatcactc atttccaaac atattttgtt tttatttcca tacactttat
     5041 atttttaatt ataacataat ccatttatat ctgcaaacat ttgttcctga tttttaatcg
     5101 tcctcaatcc attcaaaatt cttgactata tcacgtttta catttttctt atgagctgca
     5161 taaagctgag tagttgtgac attatcatgg tcaagtaagt tttgaacatc atatatagaa
     5221 aatcctgttt ggattagaga agttgcagcc gttgctctta atttatgggg actatatcca
     5281 ttgtctctag tagtatcaag tggaatagat gtatattttt ttaccagtgt tcttatagcc
     5341 tttgctgtaa ttctcttatt ttgaagagac aaaaataaag catctttttc ttcatcattt
     5401 aatagttcat ctcttgttct ttcgttttgc aaataatctt ttattacatg ttcacaagta
     5461 tgatttatag gcattaaaac ttctttccca cgttttctat atattttaaa ttcacctctt
     5521 gaaaaattaa aagaggagat attgagttct ctaagctcat ttaatctaag cccgtatgta
     5581 acaaataatg ctagtatagc tttgtcacgt aatttagttt ttctccaata taccttttct
     5641 ttctctgtaa gcccttcacc tgtttctact gattcaagca tcttagcaac ttcatctatc
     5701 tcaaggcgct taatagcatc tggctgaggt ttaggaagtt ttattggatt gaatccatca
     5761 gttatattat tatctatctg agagtttctg tataagaatt taaatagagt agagatggat
     5821 gatttttttc ttgctaacgc tctattgtta ttttcaaaaa tcaatgtgtt tttttctgta
     5881 tttttgtaat atctactgca ataatcacct aagaataaat ttatatctct tgattttatt
     5941 gtattaaaat catcttctgt aatatctttt acacaatctg catttgttaa ttctttggtt
     6001 tcaatcaaat atgaacaaaa aaaattaata tcttctaaat aagcaagtcg tgtagaaact
     6061 gcaacagaac cttttaaata tataaaataa tccttcataa atacaggaag tttattttcc
     6121 aaaagtagac atttttctat gagttcattt tctttgataa caatattatc aggtttatct
     6181 gatttattat gtacttttat aattttctta tcattattca aaattaaacc tcctaaaact
     6241 aaaatataaa tcttttatat tattttttga tatttatacc aagtgagtat aaatatttaa
     6301 tttaatcatt ttttacattt ttaagagatt agcagtgctt tagatgaaat tatcactaac
     6361 tattccctaa atccacattt aaagactagt tgaatttaag tactcttttt ttatttctca
     6421 atatatttat agaaatattt taaaatatat tgagaaattt tttatgctct actaaaatct
     6481 tttttatata atatttttat atatttctaa cgaaaccaaa tattagtaaa atatgtattt
     6541 atttaactaa agtttttaaa tgggctgact aagcttaaaa tataggatat tatgagagtt
     6601 actattgaaa aaataaaccc tattgccata aagaaactta taaatatgtt atacttaact
     6661 attaaactgg atatattcaa gttatcaaat ccaaataagt atgtaattga acttattaca
     6721 aaaataacta taactataaa gctcagacca tttaagctat tttttaaatc agcactacta
     6781 agtgacatat gtgtagatat agaatacatt aaaaacataa atataaaata cttcaatgaa
     6841 tatacactag gtgttaacaa cattattctt ataaaattgg aaatatcacc taaaataatg
     6901 tgtataaaac catttatatt taaattatta atcatactta catataactt taaatctata
     6961 ttttgagtca ctttcatata actatctggc agtaatatct taaaaattaa aataatcatt
     7021 attgttccac ctataatagg tgcaattcct ataaagaaat ttcctatact ttgataaaga
     7081 ttatcttttt tatagctatg acttacatat cctagtatac catcattttt actttctatt
     7141 ggtctgaaaa acttaactga atttatttta tgcatgaaaa tcaagcacat tatcaaatga
     7201 ctcaattcat gaacaaacgt tcctatagct ccagttacaa taattccagt ttttccaaaa
     7261 gatgaaaaga taaatttatt atttagattt tctgtaatgc taaaaatcaa tccaaataca
     7321 ataaatattc ctatgaaaca aaaaatattt attacactta tttttaaaat ttcgattata
     7381 taaatcataa gttaagtcct catgtaaaag tatttaacta ctctacttca tatccattac
     7441 agcttaaaat tttacaagca tcttctatat ctttttcttt tactaagata tagtctgtat
     7501 catacgtaga aattgcaaat atgcttattt tgttgtctgc taacaaacta cttaacttag
     7561 ataaaattcc tacaagtgaa aagtctaaag gacctagtat cttaagtatt ctccactcct
     7621 tttctgactt aacatcagaa ggaatattat tatttaaaca tacaacactt aattcatcat
     7681 cagttctagt tatagagtaa aacttctctg tatctatcca tgttggtatt ttactgtcat
     7741 tatttaatct acatactgca tactcttcta ccaataattt taatttcata atgtatattt
     7801 cctccaatct tatttgttta cttaagatta tacattaaaa gttacataaa ataaaacata
     7861 taaagtaaaa aaggttcact atagtgtcct atttctatag ccaaccttta aaacttaatt
     7921 aaattctaat aaagagatat attatctagg ttcaattata agtttaatgg ctgttttctt
     7981 atcaccttcg atttctatat cagtaaatgc gggaatgcaa actaaatcca ttccactagg
     8041 tgcaacaaaa cctcttgcta ttgcaattga ttttatcgct tgatttagag ctccagctcc
     8101 tatagcttgt atttctgcaa cgcctcgttc tcttagtact ccagccaagg ctccagctac
     8161 agaattagga ttagattttg atgaaacttt tagtacctcc atttcaacat ctccttgtta
     8221 atttgttaaa gtatccatta cctaaagtac taaatcataa ttgccattta ctaattacaa
     8281 tcatcattta aagtatactt ttataaaaat atacttctat aatcgtttta agaaaacctt
     8341 tatttttttt aaccatttta ttatgaaaaa actacaaatt tctttatatt ctataagcat
     8401 tttccaatat ttctaaattt tttatatctt tgagctataa tttcctcttt atccatatct
     8461 ttgtaatgat ttaatctttc taaaatataa gcttttattt cttttgaaac agcttctaca
     8521 tctttttgtg ctcctccaag aggttctttt ataatcttat ctattatttt taaatcatat
     8581 aaatcattac ttgtaagctt cataacatca caagcttctt tagctttaga tgaatcttta
     8641 aatagaatac tagaaagccc ttctggagaa atcacagaat aaactgaatg ttctagcata
     8701 caaacatcat ttcctactcc gatacctaaa gctcctccac taccaccttc accaattaca
     8761 aaagtgatta ctggaacaga aagtttactc atttcaagta agtttcttgc aatagcttct
     8821 ccttgtcctc tttcttctgc ttctaaacca caaaaagctc ctgatgtatc aataaacgtt
     8881 attataggtc tattaaattt ttcagcttgt ttcataagtc ttaatgcttt tctgtatcct
     8941 tctggatgag gcattccaaa atttcttttt atattttctt ttgtatctct tcctttttgg
     9001 tgtccaacta tagtaacatt aaactttcct atctgaccaa tccctccaac catagaaggg
     9061 tcatctttaa aaagcctatc tccatgaagt tctaaaaaat tagaacaaat atgttctatg
     9121 tattccaatg tcgtaggtct ctttatatct ctacttagtg ttaccttttc atatgctgat
     9181 agatgtgaaa aagcatcttc cttaagcttt tccaatttct cattaagtga atttatttta
     9241 tcacttaaat ctatattatt tgccttagaa atagttatta attgtttaat atcattttct
     9301 aagactttta ttttttcact attattttca atcaaaacag ttcctccttt atgccctatt
     9361 tcttgtgcat agcaagaatt tgatataata attccttcat ttttcttcta tctactatca
     9421 tgtcaataaa acctttttca agtaaaaact cagaagtttg aaatccttca ggaagttttt
     9481 gatttattgt ttgctctatt actcttggac ctgcaaaacc tatcaacgta tttggctcag
     9541 aaattataat atcaccaagc atagcaaaac tagcagttac tcctccagta gtagggtcag
     9601 ttagaactga tatatatggc aatgattttt cttcaagctt agaaagtgct tgagaagtct
     9661 ttgccatctg cataagagat accatacctt cttgcattct agctcctcct gaagcagaac
     9721 aaataattat aggtaaatta ttttcagcag catactctat tgaataagtt attttttccc
     9781 caacaataga acccatactt cccatcataa aatcaggatt cattacacaa ataactgttt
     9841 taattccatt tattcttcca taacctgtga caacagcttc tgactctttt gttttttcct
     9901 tataagagtc tactttttct ttgtatttag gaaaatttag tggattttga aattctacat
     9961 cagaattaaa ctccataaaa gttcctttat caatcaataa atttattctt tttcttgctc
    10021 ttaagccata atgtcttgaa cattttggac atacaaatga attttcttct atgtcctttc
    10081 taaatacatg actatcacaa cctttgcaat aggtccaaaa tttatcatct acactattct
    10141 ttttaaagtt gtcatctaaa gaaatcgtaa catacttaga ttcttttgat gataaaaatt
    10201 tttttatcat aatctattta ctccttataa tgtagcaaat tctttcttca taaagacatc
    10261 ttctataaat ttggtatgat gagtatcttc tctaaactcc tcacttgtta caagtgcctt
    10321 ttgaaattct acatttgtat taataccttc tacaataatt tcgtctagag ctctatatat
    10381 tctacttata cattcttctc tagtttcagc ccaacatatt aacttaccta tcatagaatc
    10441 atataaaggt ggtatagtat atcctgcata tacaaaagta tcaaatctaa caccaaaacc
    10501 tccaggtaaa tttaatgatt ctatcttacc tggtgatgga gcaaaattct ttgatgaatc
    10561 ttctgcattt attctacatt caatagcatg acctctaaaa gtaatatctt cttgtttaaa
    10621 aggaatttta tccccattag ctatagatat ttgaagtttt ataaggtcaa gatttgttac
    10681 catctctgta actggatgtt caacttgtat tcttgtattc atctctataa aataaaagtt
    10741 tttatctttg tcaactataa actcaatagt acctgcaccg atataaccta ctccttttac
    10801 tatatctact gcaattttat gaagtttttg tctttcttca tctgaaaggt atggacttaa
    10861 agattcttct attacttttt gatttcgcct ttgcatagaa caatctctat cacctaaatg
    10921 tattgcattt gaatgtttat ctccaaatac ttgtacttct atatgtctag gattttctat
    10981 aaacttttcc atatataaat cactattaga aaatgctgct agagcttctg aacttgccat
    11041 atcaaaattt gaaaaaagtt cttcttcttt tctaacgatt ctcatacctt ttccaccgcc
    11101 tccacttgag gcttttatca taacaggata tccaatttct cttgcaactt ctaaagcttc
    11161 ttccttagtt ttcaaaacag attctgaacc aggaacaaca gggatattca attccatcat
    11221 agtttctctt gctctagatt tatctcccat caaaatcatt tgttctcttg ttggacctat
    11281 aaaaataata ttattatctt cacattgctt tgcaaattca ggattctctg ataagaatcc
    11341 aaagcctgga tgaatagcat cacactttaa tttaagagca agatagatta tattttctat
    11401 attattataa ctcttactta tattatttgg tcctatacaa atagattcat cagctatata
    11461 tctatgaaaa caatctttat ctatttcaga atagatagca acagttttta tacctagttc
    11521 tttacatgtt ctgatgattc taactgctat atcgcctcta tttgcaacta gtattttttt
    11581 tatcaactta aatacctcct actacaatgg ttttatctta aataaaggtt gattatattc
    11641 tactagttct tcattactaa ctaatacttc aattatttca cctttaattt cgcttgttat
    11701 ctcattcata agcttcattg cttctaatat acataaagtg tcgccttctt caactacatc
    11761 gccaacttta acaaagctat ctgcatctgg acttggagaa tcatagaatg ttcccatcaa
    11821 aggagctact actactgata gattctcatt agatgtgaca ggtgtatcgt ttacaactgg
    11881 cttttctaaa gaaagatcca ctactgattc ttgttgtaca cttaaaacag gtgaacttgt
    11941 agattgagct tttttagata cttctaatct taaatcgcta ctttcaagtt ttacatattc
    12001 taagttagtt gagtctatgg cctttaataa ttccttaatt tcattgatgt tcattttgtt
    12061 acccccttgt attattacct ggtgctaata ttatatcaca aaaaacaaat ttataaaaga
    12121 aaaagtgctg actagagaat caacacatca atatatcttt taattttaaa ttaacaacca
    12181 gtaacttata tttgttttaa ttttccatta ttaatataaa ttataagtat aaaatgaata
    12241 aaattcaata ttttttgcaa aatacaagag aatacaaatt aatacctcgg gaatttttaa
    12301 gacaaatacc aatattatgc aatttagact tcgctttctt tcaaattagt atcattttct
    12361 ttatttttac ataggatgta ctttaattta gctatcaaaa cattactata taggttaaaa
    12421 gttattagaa ttgcaggaat tataaagtaa actctagcac ctataaaacc aaaaactact
    12481 gtaataagta ttaatctttt atttaaagta aaaaaaggta tattaaaaat acttttgtcg
    12541 gatgtttctg gagtataaga atatataact ccaaaaatca aagtaagcat acaaagtgga
    12601 agtagtgtat aacttgaaat tattcccaga aatcctatac tttcaaaata tttgctagca
    12661 ctatttgtat aaacaaacat gtatccattt aaatatgcgc atatacatga tataatgcca
    12721 aaagttcccc ctaaggattg agtgtaccaa ttaaaaaatt tagattccat atacctcacc
    12781 tcctaaaact gtatatataa tgatatgcat ttaaatagta aaatatatct ttatatgttt
    12841 ctgaataaaa ttttgttaat ttcattaatt tatgtttaag aaatttgaag tatagtgtag
    12901 tatatattaa tgtagatgaa gttttatagt acttatttca tagtgcttaa tcaagttatc
    12961 ttattatgga ttcaaaagtt gtttgtaggg ttttatattt tgttgagctg aaaaatttat
    13021 tataaatgaa gggatgtttt cgctttggta gatacattag aatttggtct taaaatttta
    13081 ttttttatat tatcaataat ttggatgggt aaaataatga ttttaagaac tgataagcaa
    13141 atagtaatca atcctttgct tattggtata tctgcagtcc ttgtgatgtt gcatacctca
    13201 cagagtaaca ttgaattttt tggattagat gtccaatata taagaatagt tttgtatata
    13261 atatattcac tgataatttt aataggaata tgggctacta acagaagaaa tggaattttt
    13321 tagataatct atttctataa aatttaaagg ttgctagtaa caaagcacta atattagcgt
    13381 tctgttacta acaaccttta ttttgattat acacttaaat tgaaaataga atgattgaat
    13441 aagttgacat attattttac attgacagtg attagaactt cttgagatgt atttaaatca
    13501 tttttgtcgc ttagtataac ttttaccaca gcttgacctg ttttattacc tttaattatc
    13561 atatcttcac atgaaagtac actcttatca cttacagata caataaagtc taaatctttt
    13621 ggaagattat cagtaacttc ccagtattta taataactat caattttctt cgtaattatt
    13681 tttctcaaat caatactttc accttttgct atatcaacat tatgatattt agagtccagt
    13741 ttatactgat tatcttttgt atcaataaaa tttaaatcgc aatttacatc cacaccatta
    13801 tgagaaagag aatttactac atcgtcatca atcaaataat tattggcaag atttattgaa
    13861 tccatatctt ttagacttaa agcttcctta ggaacctctt ttatattatt attccaaaaa
    13921 ataaaattgt ctacattttc aagatatgat attcctttta aatttgaaat gctctcacca
    13981 gttgcataaa attctccatc taaagccttt atattgctta tagtcatctc atctgggttt
    14041 atatatcttt ttacaactgc tcttaaattt gcatcaggaa aaatatcttt aagttgattt
    14101 tcgctcaaaa ccttatcatt tgaatttatt ggacttaaag ctgctgcaga aataataaat
    14161 ggtattaaat tagctaaatt catagtaccc tcccacataa attttttatt ataataattt
    14221 aatttctatg agagggttct gtttccttgt tatatatacc tactttcgta cactttatta
    14281 atataaatta aaataaattt ctactattaa acttataaac atattactag ttacgttttt
    14341 tagcttcaca tctaaatatt cgcatacctt gtgacataaa tttgtcttca tactctgttg
    14401 taacattatt ttcatactca ctatttccta aatcaagaga aatattcttt aatagccaat
    14461 tatttgcagc aatttcattt aaactaaact cgaataattt ttcattatct gttttaaagt
    14521 gaatttcgcc atcatcttcc aaaaccctat tgtacatttc taaaaatcca gaatgagtga
    14581 gtcttctttt actccatcta tttttaggcc atgggtcaca gaaatttata taaattctcg
    14641 ataattcttt agcttcaaag taatccaaaa tattgcttac atctccccat aaaaatagta
    14701 tattattcaa attagaagca tctgcttttt caacagcttt taaaagaact tcttccttaa
    14761 gttccatggc aatataattt atgtcagggt tctgcttagc taaagttgtt ataaacttac
    14821 cttttccagt cccaaattca acatgtattg gattatcatt tctaaacttt aaattccact
    14881 tgccttttaa tttgtctata tcgtctctta aaacatattt tgtataactt aaaagctttt
    14941 catctgcacc tttttttcgt cttcttctca caataattct ccttacatat aaaaaattag
    15001 taaaatacat ttttattata cattatctca ccaaaaaata aaaagaactc tcttaaaata
    15061 gaaatttatt tctataaaga gagttctaaa cttactaaaa acttaatctt agtggcaccc
    15121 tgaacaacca gagcatcctc ctccaccttg atctttcata ttttcaggaa taactctaaa
    15181 tccaaatcct gtatcttcta ttattatatc tccatactga ctatattcat cagagctcat
    15241 tacaaattgt aattctccaa cttcataagt taagtcatct tcttttttct catctaaagc
    15301 taatccaaaa gaaggacctc cacaaccaac acctgcaaag tatactctta tattattagg
    15361 tttatcttga ttgtctttta agatactttt taaagtatca atagctgttt ctggtaaagt
    15421 aattttcatc aaatcattcc ctttcaaata ttttattaaa acaatatcta ttatataccc
    15481 atacaaggta cataatcaat tagtttttgc atattttact aaaacttcta cctaatatta
    15541 cctaaggctt taaaatattt tatataatat tttattacct taaataaata ccctatcata
    15601 aacttagtaa acaaataaaa ttattattct aaaaaatata ataattttat ttgttgtttt
    15661 aatattataa cacatttgat aatgtccaat tcatatatgc aaaatttata tactttaaaa
    15721 tatctaagaa attattcaat aaaaaaatac tattttttaa aaattatcat tattgataaa
    15781 gctctactat ttatattttt tagtgtattc atgtataata agtaaaagca caacggtata
    15841 tactgttgtg cttttttgta attaataaac aatattttgg tctataacaa tgaaaaatgg
    15901 ctactttcca taattgattt catctgacat aagcaaaaag ggtgtccagc tgggtcaatc
    15961 ataactctcc agtcatcagt aaattgctcc tctgcgattg tagctccaca atgaatttca
    16021 tattgaactg cttcttctaa atcattaaca gcgaaatcca tatgagccat ttgctgttga
    16081 gattcaggtt tttctggcca cacaggcggt ttatactcag aatttcgttg aaatgttata
    16141 ccaggatatg ttccctgatt tgttcctgga gcaccaacac atgcatattc ttcattataa
    16201 aatgctattt cccaattgag taacgctgca taaaattttg ctaattcatg tgggtctttg
    16261 caatccatcg taaatgagta cattttgatt ttcagttcat catgcataac gtaattacct
    16321 ccaagtataa atttcttcgt cttttcattt ttaagctaaa ttcaaacatt aagtatgtaa
    16381 tttatttcaa gtatttttct attatgcccc aagcctttgt tttttgcaat ataaagaaaa
    16441 aattatatat tttaatattt tataattgtt gaaattgtgt aataaattca actggataca
    16501 tagtgcgttt tgaaatttct tctaaagcta aaccttgttt taaaagcctc tttatgacgc
    16561 tttccatact ttctgaaatt tctataatat ttttatcaaa atcataaaac ctaaaagttc
    16621 tttgacccca tgggtattct tttatataat gcaaaaattc aatatttcct attctattta
    16681 tcttactttc ccattgtttg atatcattaa cctcaaaata caactgaaag ttatttgatt
    16741 gttgatatat tggtaaatca tatccaatta tagctttata attactttgt aatccaaatc
    16801 cattttcaaa tgatacatgt tctcctaaat ctaatgtaat tttttgctcc ataacatctt
    16861 cataaaactt ttttgattta tttatgtctt tgacaattag caatactcct ccaaatttca
    16921 tcttatcact atcctttctt tgttataagt attataactt aaaattttgc aatttcactg
    16981 taaaaatcag acatattagc tcggtatttt tgaggagtta ttccacagac aattttaaaa
    17041 tctttaataa aatgtggtat atcatagaat cctaattcat tacatatata catcaatgaa
    17101 gttttattat tgtttagaag ttttatagat ttattaatac gtaccaaacg agaaaatgac
    17161 ttcatactca accctaggta ctgattaaag agtctgttta aatgcctaga actataaaat
    17221 acttttttag aaatttcttg tgaagatatg tttccagagt tttgaataat caactgaatt
    17281 gccaaaataa actcatatga gtattcaatt ttaatattag aaatcaataa ttcttccaac
    17341 tttaaaatca atttatctat agataaagat tcattaaata tttttttaat ttctaaatct
    17401 aatgaagtat taattataga aaatggaaca actttatcaa taagttcact ttgctgtata
    17461 cctataagcg ggaaaaaacc agcaggttga aattctataa ttagtataac atcacatcta
    17521 ttagcaatat ttcctactgt ttttggttta gtagttggac caaataatag actatgtaac
    17581 cctgtggcat cataaaaaaa caccaaagtt acacttccat gaggaataat cgtgtaatta
    17641 tcagaaatta ttgtttgatt tggaaacgat atagtaaaat tcgatatcca attttttaat
    17701 tgcatacaag gcaagagata aacaaaattg tcttcacaaa ctataagtga cctattttta
    17761 tttataaaat gaatactttc tttttttaac atatattata tcctgtccta tcgtgaatat
    17821 ttattaaagt aagaagaatc catattatct ctatatatca ttaactgctt ttaataatag
    17881 agatagtatg aatactcatt agatatttaa taaacaaata aattaaaata atcaataaaa
    17941 aatatagtac ttttatctgt tattttaata ttatagcaca tttatagtat ttaacttata
    18001 tccactttac atatgtctaa tttatatagt taaaaatgct tatctcaaat acaataatta
    18061 gtccttatac aattaaacca tctgaaactg taatagtacg actactactt ttggctgctt
    18121 ctggtgagtg agttaccata attatagttt gtccattgtc tctgtttatt ttattcagta
    18181 aatctattat ttctatccct gttttactat ctaaatttcc agtaggctca tcagcaaata
    18241 aaatctctgg ttttccaact aaagctcgag ctatagcaac acgttgttgc tgaccaccag
    18301 ataactctcg tggtgtatgt ttacgtctat cagttaaacc aactatatct aagatttcat
    18361 ctaattgatt tttataatct tttaaatttt taccatctaa taacaaagga agcattatat
    18421 tttcctccac atttaaattt ggtattaagt tataaaattg aaatacgaat cctatatttc
    18481 tacgacgtat tatgctcatt ttctcatcat taaaatgtga tatatcagct ccattcatat
    18541 acacttttcc actcgttgga gtatctaaac cacctaaaat atataaaaga gtacttttac
    18601 cagaaccaga ttgacccatg atagaaacaa attcaccttt cattacttgt aaagatacct
    18661 cttttaacac tcgagttgtt gtatttccaa ttttatattc tttgataata ttttttgctt
    18721 ctacagctaa actattatgt gtaattttca tattaacctc cataattatt caaacttaat
    18781 ttcttctatc agtttcattt tcttcccttt tataataggc actatcgaac ctattaaagt
    18841 tactataatt cccaataacc caacaataat aaatgtttta aaatctaaat ctggtttcat
    18901 tgaaattttt ggtcctgcaa ctataaagat agtttgaatc tcaagtatag atatcacaat
    18961 accaataaca gctccaagta aacctgaagt aaacccttca atcaatgtca cttttatatt
    19021 ttgtttatca cttagtccta tagatttata catagctata gaacgttttt tttgaatata
    19081 attaatcagt agattattaa taatacctac tgtagcaaga agaaaaatga aataggtcat
    19141 cttattcata ggagacaaaa aactacttat agtatttaaa gaatcattat taaattcttc
    19201 aactgtacga ctccaattat atgtatctcc aaataaatct cttatctgaa tcataacaac
    19261 atctggattg actgctgtat atactaaaaa accataatta gttttatcaa aatcattaac
    19321 tgcataatgt gatggtataa cagcttctac atcatttgca cgggatttaa aactacctac
    19381 tattttataa gaaaaatcat acttatcatt agatagagtt attgtatctc ctatagataa
    19441 gcctactttt tttagagtat cttcattcaa aataacagaa cgtttatcat taaaagcttc
    19501 tattacttgt ttttttatct caaaatttgt ataattgatt ccaaacatag agttatatag
    19561 ttttatatta tctgtaccct ctagtcttga taatgttaca ccattaccag aaatctcatt
    19621 attcataaca taaagaggta atattttttt aattccatcc atatgtctaa cattttcaat
    19681 aaactcttca ttcatctttc catctgcaaa accttgtaat tctgcgtcac gaaacacatc
    19741 tgttatataa gttttgacaa agttaccaac aacacttatt gcaataactg cagaaatact
    19801 tataaatagt aaagtgatat tctgtattat atttttgtta tttttcatat ttctagcagc
    19861 taatttaccc tcattcccta aaatattttt ataaacaaac tcaaaaacta tagacatgat
    19921 atcagtaata agaggaatca acacaatagt agctacaatt aatcctagta atgaaaatcc
    19981 accagctaga tacaatgtat tttcagggga tattcttgga agtaaaatag ataaaataaa
    20041 cattatactt cctataaaaa gtatagaacg atttgaaaca ttcttttctt ctactgttcc
    20101 tagtacaata ttttttattg gcaaatggct agctttttta acaggtatat atgcactaaa
    20161 tgaagaaacg attattgcta ctataacaga tataatcact ccaatgggtg atattactac
    20221 tggaattgat attccttgtt cgagagaatt tcctaaccca tgaagcatta aatttaaaac
    20281 aactacacca ataggaatag caattaatcc tcctatactt ccgtataata tactttcaag
    20341 catcaatata cgagttacag ttttttcatt ggcaccaata cttctaaatg ttccaataaa
    20401 aggaagacgt tctaatgtaa taaccttata gctgctgtat atgataaata tactaagggt
    20461 taatgcaaaa aaacttatta aaaagaaagg cattgtcttt tgtctagcat ctgaaattat
    20521 cttagtttca tttatagtat ttgataccat atatttttca gtggaaagtt cttcggataa
    20581 tttatttact agattttctg tcatttcttc ttctgactct ataagaactc ttgtatatcc
    20641 actgccttta tttataattt ttgatagagt ctctttgggt agcagtgcat ttacacctct
    20701 tgtattcctc aaaaatactg tatcataaga tgcaatatca ctaacttgaa aagtataaga
    20761 ctttccataa atctgtaaag taatagaatc accttttttt attttatatt ttgatgtaaa
    20821 gcgatttgga agaataattt tatcaccaga aaaatcagta atactatcgc cattttctaa
    20881 tcgtggcttg ttgattttat ttagttgact taagtctgct gatataagac taaattcttc
    20941 atagtatccc cctttattat aaatagcaga gctttccaat acaccaacct ttgattttat
    21001 agcatttaaa tcaggtatgt cttctaaatt ggttaataca tctggatttt tactttggac
    21061 agatatagtc gctgtaccat acataccttt tgccattttt ctttgtgcac tttcataaga
    21121 taatccaatt gagaatgaaa caaaaagtaa aactgttgat aaaactatgg acagtaacat
    21181 aactgctgtt ctaatttttc gttctttaat atttgtgaaa atatacttta atataatttt
    21241 caaatttaca ccttcttcct ttatctaaga tatttcagta taatattttt atactttaat
    21301 ccaaattgaa tctctaatag actcctccat acttttatca ttctatttat aatattttta
    21361 atcaaatcac ctccttaaat aaaaaagatt gataaactac tatataaata taataatcta
    21421 tcaaccttaa tatatttttt atttgacctt aattaacctt aattaaatag gaaaggtgat
    21481 ataaaatgag ctacctaatc caggtatact ttcaacagat atattccctc catgaacttc
    21541 aataatttgc ttcgttattg ctagtcctaa accagttcct tctatttttt gttttgtatt
    21601 gcttcctcta taataacgat taaacaaatt tgatgcttct tctcttgaca tcccttttcc
    21661 attatcagat ataatgattt caattctttc atttaatgtt atatgtacac aaactttagt
    21721 atttttatct ccatatgtaa aagcattgac aataagatta ctaaaagctc gttttaatag
    21781 tgtacaatca aaatcaaagt taatattatc tatattactc ttaaattcaa ctatatttga
    21841 ttcatattct ggataattta aaatatcaat ggataattct ttcaagaatc ttactaaatc
    21901 atatttattt ttatttaaag gaattattcc attttttaat tgataggtta attttaaatc
    21961 gtttataagt gtatttgtat attctatatt ctttaacatg attgaagcat atttatatac
    22021 tttttcaggt gaataattag tactactatc tgtaagaagc tctgcatacc cttttattgg
    22081 tgaaagtgga gtttttaaat catgagttat atttgaaatc cactcttctc tcataatatc
    22141 agttttattt tgtatctcat cacttctatg aatttcttca tttaaaatat ttaaactatt
    22201 atatatatca gcaaaaacac ctttaatatt aactggaaga tagcttcgtt ttgaaatatc
    22261 actaacagca gatgtaatat ttgatatgca ctttgttacc cagtaaccat aagcaattcc
    22321 agaaatcaaa attattaaaa atataatccc tatgattgaa aatacaattg acttaccacc
    22381 tacaaattta cttccatcaa aaaacatatt cactttagat atatttaatg gaaaatatat
    22441 aatataaata tagtcatttt tattattttt aattgtatta aagtatacag ttccattact
    22501 tgaattattt tggttagcta atgttaataa ttcagaatta gaataataat ctttatgctc
    22561 ttttggttct aaaaacccat aaactctatt tccactagaa tctaaaattt gcaaccacaa
    22621 ttgattttct cgaagctttt ctaatccaga ctgttttact tgtggcttat tatctataaa
    22681 tatgatttgt tcattaaagt tttctgtaaa ttttttcggc cagtcactac gaataaattc
    22741 cccatttggt ttctgaatag taataagtga taagaaaata cctattactg tcaggattaa
    22801 gcaaataaaa atcatgaaaa aaataatgta tgaatgaaat actgttttat aactaggttt
    22861 atgcatttaa tcaactcttt tcttaaactt ttattttagt ttgattgttg cttatataaa
    22921 atagataata attattgata tgtaaaaatt tatttaaaaa tacaactatc tacataaatt
    22981 atatttatat aattatatgt tatcaagctg tctgttgtat ttaataaatc cttttgataa
    23041 catataaaag caagataatt ttttccatta aataccttat aattatacac aaaatctcta
    23101 tatacgttaa acagtataga gaaaattttc atagaaccat ctttataggg gatactttat
    23161 aaaagaataa aaaaattata atatataaaa aacttcatag gagtgtaaca acatcagtaa
    23221 agatatgaaa acactatata aaaattcaaa aatattaaga ataatgaaat acttgaaatt
    23281 gcttatatca agaaatttta tataatataa actggtcaaa tatgttataa tattaaattg
    23341 tgacaagttc atggaggtgt ttatatgaaa aaacaaaagt cgccaatttt acttgaaatg
    23401 cttggactat ttttgggttg tatatcaatg agtattggta taaatatgtt tttaaaacca
    23461 catacaattg ctcctggagg acttagtggt ctatcattag tactaaacaa ggttacagga
    23521 cttccagtat cagctatcat gttaattata ggtgtcccat tagtaatact tgcttttaga
    23581 attatgggta caaaaaattc attaaaaact ttatttggaa cagttgtatt ttcagctata
    23641 gttcaattaa cagacccatt atcaaaacta agatttacta atgacttatt attgtcttct
    23701 attgcaggag gaatcctagt tggtataggt ttaggaataa tgtttaaatc agatgcttct
    23761 actggaggaa cagacttaat tgcactcata cttagtaaga aattcccagg aataaaagct
    23821 acaaaattca tgtcatgcct agatggaatg gttgttatat cttctggtat agtaaatcgt
    23881 agtttagaaa ctgctcttta ctctggtata gcactttgtg tattaataaa aatagcagat
    23941 atgataatgg aaggatttga ccattcaaaa gcatttttta ttatctctga tgagcctgaa
    24001 agtttaagac aagctataac tgaagaactt gatagaggtc ttactattct tgatggtaaa
    24061 ggtggataca ctagagaaaa taaagaagtt ctattagtcg tagtttctaa aaaacaagaa
    24121 ctttatctaa agagattagt taaaagaact gaccctactg catttgttat tgttacagat
    24181 gtgcatgagg ttcttggaga aggatttaaa aatttagaga actaataaaa attaatattt
    24241 taagtatata tactatttta attaaaatac gatttattca aaaagagtgt ctcataaact
    24301 ttttagttca tgagacactc ttttttgtat aaaatcaaat gcattttcag tattttaaca
    24361 ataaaaaaca ggattatcta tattttgagt caactacttt attgaaattt tatttagaaa
    24421 acaatcatta ctaaataacc aggatagata ttaaatgatt atatctattg ttttttcaat
    24481 tcatattgat aacaaggaac attttttaca gtatttccag tatgaatttt tttgatatac
    24541 tttcctgtac atttaaatcc taataaatct aatgattttg tagaattttg gttttcttca
    24601 taggttattg caattatttt atttaagttt agttctctaa atccatactg tattactctt
    24661 tttgttatct cagtagcaaa atcatgtccc caatagcttg gatttataca tatacctatt
    24721 tctgcttcta atgctttagg atttatatta ttaaacccca tattcccaat aactttattg
    24781 tctcttttat atactatagc aaagtggcca attttccctt ttttataggg atttagaaaa
    24841 aatgatttta taaactttct tgtatcttct aaagatttat gctcactaaa tggtaaatgt
    24901 tttactacta ttttattttt ataacagtca aaaaaatctt caacatcatc taatgatgca
    24961 ggtcttagta tacaacttct agtttctaag gttttcattt gaggatattc agaatcagac
    25021 atttctaatc cccctttata cattaatcta aatatactgt tcatatttaa aatacgatat
    25081 taattatata aatgttctca aattaacctt atctttattt ttaataaaaa ctaattacta
    25141 tatgaatctt tcaaatttac gaatttatct ctatttttat attaaattac ttgtgaaata
    25201 tgtattctaa attatatgtt attagatatc atgcttaaaa attctttggt tgctaaggat
    25261 aaagatatat cttttaaatg gcagtaacct atatttctct taggtattgg ctcttttata
    25321 tctaatttaa acaatttctc attttccaaa taatctatgc taaattcttc tataacacaa
    25381 gacactccaa gatttatata cgcaaattct aaaagtaatt catgagaacc caattctata
    25441 tccgggttta gttttatacc ttttgataaa aaatattcat ttacataaag tcttgaattt
    25501 gctttatttt ctagcattat taaaggcaat gtgtttaatt cttccaatga tattttccta
    25561 cctttgtatt ctatgtaatc atttgcacat acaaatatat catgtatttc aatacactca
    25621 acaatattta gtgtatcatc ttctattggc atgtttataa ttgctatatc tatattccca
    25681 tctttgagta aagatataag ttctctactc gttctattta ttgtagatac atgtatatgt
    25741 ggatacttct tatgaaaaat ttctaaatat tttaacaaga aaaaacgtgc agctgtatct
    25801 cctactccta ttttcagaga accgtattct aattttttaa atttatttat tctatcttct
    25861 cctgatgata tcaactttaa tgcagtagtg acatgttcac ttagaatttt cccctctgga
    25921 gttaatttta ctcccttatt gtttctataa aataataaag tatctaattg ttcttctaat
    25981 tgctttattg actggcttac agctggctgt gacataaaca attccttagc agcaagagaa
    26041 aaactcttat tatttacaac tgcattaaaa accttataaa gttcaagttt tatattcata
    26101 tataattact ccttatgtca aatattagta ttatttattt tacttataat tatatttgaa
    26161 ttataatata catggtacta atatgacaat attaattctt taggaggatt ttagataatg
    26221 gatagattag ttggaactgt ttcaagaggg gttagagcac ctattataag acaaggagat
    26281 gacttagtta aaatagttgt tgattctgtt ttaaatgcgt caaaaagtga aaactttgaa
    26341 gttagggaca aagatgtaat tgccgttaca gaagctgttg tagctagagc acagggaaat
    26401 tatgctcacg ttgacaatat tgcaaaagat gtaaaagata aatttggaga tgatactgtt
    26461 ggggttatat tcccaatttt aagtagaaat cgttttgcta tatgtttaaa aggaattgca
    26521 aaaggatgta gaaaagtcgt attaatgtta agctaccctt ctgatgaagt tggaaatcac
    26581 ttaataagta ttgatgaact tgatgataag ggaataaatc cttggtcaga tgttttaaca
    26641 gaagaaaaat acagagaact atttggatat aacaagcata catttactgg tgtagattat
    26701 gttgattatt ataagaattt aatcaaggat tgtggtgcag aggtagaaat agtatttgca
    26761 aacaatccaa aaactattct taactataca acaagtattt taacttgtga tatacataca
    26821 agacaaagaa caaagagaat tttaagacaa aatggagcta aaaaagtata ttcacttgat
    26881 gatataatga cagcaagtgt agatggtagt ggttacaatg accagtatgg acttttaggt
    26941 tcaaacaaag ctacagaaga aacagtaaaa ttattcccta taaattgcga tgaagttgta
    27001 aacaaaatcc aaggtaatat aaaagaaata acagggaaag atgtagaagt aatggtttat
    27061 ggagatggag cttttaaaga ccctgtaggt aaaatatggg aattagctga cccagtagta
    27121 tctcctgctt atacaaaagg gctagaggga actccaaacg aagtaaaatt aaaatactta
    27181 gcagataatg attttgctga cttatctggt gatgagttaa aagaagcaat ttcaaaatac
    27241 atagttgaaa aagataataa atctgatgat ttaacaggaa acatggtttc tcaaggtact
    27301 actccaagaa gattaacaga ccttataggt tctttagcag atttaacatc tggaagtgga
    27361 gataagggta ctcctattat ttacatacaa ggatatttcg ataactatac aaaatagtat
    27421 gttttaataa gatttaattt ttttggatta tatatattaa catgaaaatt tattaaaacc
    27481 tcttaataga tatgactttt caagagttat atctattaag aggtttattt aatgttcaca
    27541 gataagatat atttaaataa tttaaacaag attgtcataa agttatataa tcttaaaaat
    27601 ttatataact ctaactctta tttatttctg aaataatatc actcttcaaa atttgtctta
    27661 ctggaataat tacagcaact atactaatag tgatatttaa aataatcata cctatgtata
    27721 gaggtgtatt tattttaaga attggattta tatataaata tacacgtttt accataaaat
    27781 aaatcataaa ttgtccaata acacttccaa ttaccattat aatacttgca tagaatccat
    27841 ataaaaaacc ttctcttatc atcataagta aaaacttatt atccgtaata cccatagctc
    27901 ttaaaattcc aaactcatgt cttcttgaaa aaattaagta gcttactgta ttgattatat
    27961 ggaacaaact tattgcaaga agtacaaata caataccata tataaataag agtttttgat
    28021 ttaagtaggt attatttttt tcaatagcaa tggtataatc tgttacgata catcttttta
    28081 catcacttac aacacttttt atgttctcag acaattcctc actgttacta tcttctgtct
    28141 tttttatact tactgaatta taattttcga tatcaaagtt atttttcatt tgcccattcg
    28201 tcataactat atccatacca taatcaccaa tgaaatatat gttatttgcc atcacacgtt
    28261 ttacagtagc agcaactaca aattcttttg ttgtataatc attcttattt tcttctttaa
    28321 atttaacaat ttcacctgtt ggtttcattg tctttggcac tttaactttt attgtatctc
    28381 ctggatttat atcaactgca tcatacatcc cagttccatc cataggtagt ctaactataa
    28441 ccttattatt tttaaccata tcatgggtat taatctcccc atctataata taagaattta
    28501 atccttctaa cataccattg ttataaccat aaatatttgt ttttaaaaga tagccacctt
    28561 cattttgctt tgtacaaata ccatcaaaaa aagtctttat cctgtaatct ttttcaagag
    28621 gtttaaagaa atttttccaa aatagttgtt cttctttaat ttgcatagct ccaaataaat
    28681 atcttactgg atagactttt gatattccct caacattgtt taatcttgaa attttatctt
    28741 ttggtatacc tacatcaaaa tctgttgttt gcatagttat ttggtagtca gaatttaaac
    28801 catcatcagc tttcattgta agttcattat tgtttttagt aagctgtata gagtaactag
    28861 aacatagaaa tataattcca ccaagtgata atgaaaacaa aattccaata aacattcccc
    28921 ttttttgagt catgtattta tgagataata cttttgtcat gttagagtgt tttacacttt
    28981 ctaatattct ctttttctta ttagtaatct taaagttttt catagattct atactagagt
    29041 atttattcaa ctgatgtaca acacgtacag caattaaaat taacaacaat aagataaata
    29101 aaaagcctaa ataaatagat ttttcactta taaaaaatgc tcctggaaca atatcactac
    29161 tcaaaaatat atgtggaaat tgttcatata ttgtagacgc tactccaata cctaagaagc
    29221 aacctatagg aaatccaaca ataaaaagta aaaataactc taaaaatagt agaaataaaa
    29281 gttgtttatt attagcgcct aatacttcta aaaccccata ttgtgatatt ctctgaagta
    29341 ttgaaacatg gaaaatacta tatactgctg ctgcaccaaa cactccaaca agcaatataa
    29401 ttgcatttgt atttagttct aaatttttta agaactcaaa tatattatat ttttctatat
    29461 tctttggtgg ttttactcca aattcaactg aaagaggcca gtttatataa gttttttctt
    29521 ttgatatgtt gaattttttc ataaatttat aattttgctt tttaatattt ttattttcat
    29581 catattttac ataaactata taagaatcct tttctttagg ctgcaaataa tcgtttacaa
    29641 atccctccat agcagtttct gggtttgaaa gacttgataa aataccacat aacttataga
    29701 ttttaccatc taaattgatt tctgttccaa tttctttgtt tacatttaaa ttgtttagag
    29761 aatatgtgtc aagtactatc tctccatctt tactaggaag agttcctttt aataattttc
    29821 tacctgtcat gtctaaatat gaagaatcag catttaaaaa ttctataata aaaggttcat
    29881 ctatctgatt tttaggtttt gtaacgccaa gtctatctac aatgtattcc tttgattttt
    29941 tatttttttt aattttatta agttgttcct tatctacttt atagaaataa tgataatctc
    30001 cactatcttc ccttatcttt tcaattctac ttttgtctgc actatgaact aaagaaccaa
    30061 ttcccgtcaa taatgctact gatagtacta tgcttaaaac tatagatata gattgttttt
    30121 tataatgctt tatatattga attgaaaatt ttactagaga catcatagta cttcaccacc
    30181 agtattcacc acaactccat cttcaatatg gataattgag tcgcatactt gtgctatatt
    30241 ttcattgtgt gttaccataa gtatcgtttg atggaatttt gcactacttt cttttaataa
    30301 acataccact tccattgtag ttttactatc gagatttcct gttggttcat ctgctaatat
    30361 aagtgcaggt ttatttgcca aagctctagc aattgctacc ctctgttgtt gaccaccaga
    30421 tagttcgctt ggaaatttta aacgcttatc ccaaattccc aatgtcttca tcagcatttc
    30481 aatatattca tggtctatat ggtttccttt atctaatgta acaggaagag ctatattatc
    30541 ataagtgttt aatacgggca ttaaactgta gttttgaaat ataaatccta tatttcttct
    30601 tctaaatact gtcaaatctt tttttcctat tttagatatg ttctttcctt taattattac
    30661 atcacctgag gttggattat ctaatccacc tataatattt aacaatgtgc tttttccaga
    30721 accagaagtt ccaactatag cagtaaatgt atctcgttca atttctatac ttactccatt
    30781 taaggctttt actttagtct caccttttcc atagtgtttc actaaattat tcgtcactaa
    30841 tatactcatt taatttctcc tttcaataca gatatatgtt ctataaaata ttgtaagtga
    30901 cgataattac actcatccta caaagttagt ttaaactctt acaattctgt aagaatatac
    30961 taaaacaaca tccacttcct agtttagatt ctactataat gtttccattt tctttttcta
    31021 aaattaatct tgtaagatat agaccaatcc ctgtgccatt ttggttttcc acatttttac
    31081 ttctgtaaaa tcttttaaaa atatcattta gttctctact atcaatacct attccttcat
    31141 ctttaatagt aatcttcgta tatagctcca tttttattac agaaatagta atcgtgctat
    31201 ctgatggtga gtattttatt gcattttcta aaacatttac aattgcttct atggtccact
    31261 ttttattatg aactaacttt atatctgaaa atggttcgag atttatcctt atatttttag
    31321 cttctgattt attaaagata gtactgatgc tttgaataag agtatcttta attagtagtt
    31381 cttctacttc aaattctata actccatcct ctaatttagt cattttaaat aaggattgta
    31441 acaaccagtc aatcttaaaa gtttcatttt tcatcttttg taaaaactcc ttcttctgta
    31501 atgtggaaat atttatattc tccagaagtt cacaataaat ggttatattt gaaagtggag
    31561 tttttaattg atgtgacata tttgatatta aacctttaat cgcttctttt tctgactttg
    31621 actgttctac ctctatttct atcatatcct taattttttt tacttggtga gaaatttttg
    31681 acgctcttgt atccttcgta tctggataag taacttcttt accttccaat atttgattta
    31741 acatcaaatt taattcatca tatcttcttt gatttatttt agtagatact ataaatataa
    31801 caactgtaca tattataaag gctaatataa acatattatt ctacctcatt ccataaataa
    31861 cccattccat aagctgtttt tatatatttt ggattagatg ggtcatcctc tacttttact
    31921 ctaagtcttc ttatatttac agatacaata ttatcatcaa cataattttc ttcgctgtcc
    31981 caaatatggt gtaaaatttg ttcctttaat aatatctgac ctttattttc aattagataa
    32041 cttagaagct tatattctac agagctaagt tccaaacatg ctttattttt atatactttt
    32101 agtaactttt ggtccaattt tattccattg gaatatatta ctttattatt cgattttttt
    32161 cttattgcta ctttgactct tgcctttaat acagataaag aaaatggttt tgtaatataa
    32221 tcatcaagcc ctatttctaa tccttttact tcatcaattt ctgagtctct tgcagtaagc
    32281 attataattg gtatatctga cttttccctt attatttgac atacatcaaa tccatctcca
    32341 tctggaagat tgcagtctaa gattataaaa tctatatttt tactattaaa tatatttaat
    32401 ccttcttgtt ttgtataagc cttaaataca ttaaatccat cattctcaaa tgcataaaaa
    32461 agaccttcat ttaagtttat atcatcttca ataattaata agttcaattt tactctcctt
    32521 tagatttttt agataaataa ttattttctt gcattatcat actaaaaata ttttaatttt
    32581 atttatttca ctaaaaagat tgtatttaaa tttgttttca aaataaaaac atcataatca
    32641 agcatatagt tatatatttc ccaatttaat tacgcttaaa ttgaaaatat aacgttattc
    32701 ttatataatt aaattataca acaataaagt tatatttttt aaatatattt ttactagggg
    32761 gaatagaatt gaaaagggtt gatgagagaa atactatgtt tgcaagagca aattacaaaa
    32821 aagatagtac tgcttataat gattattata agaaaaatcc tgacaaaaaa gaaattgatg
    32881 atagtataag aaatagacca aatttatgta gtgaaggaac aatgacttac aatgagttaa
    32941 actctccaat ggcaagtagt gcattcgatt ttttatctga tatcaaatct ttatgcgaag
    33001 gaaaagtttc tgatactaaa gtagatgtag atgcaaagtc aatgacaaaa aaaattaagg
    33061 gtcttgcaaa gcagtatgga gcttctgtag ttggtataac taaattaaaa gactatcata
    33121 tttatacaaa taggggtaga catgaagaaa attacggtga agaaattaat ctaactcata
    33181 agtatgcaat tgtatttgga tgtgagatgg ataaagaaat gataaataga gctccaatga
    33241 tatgtgaagt tatagaaaca tcaaagtgct atgtggatgc atctatcgtc ggcatgattt
    33301 taagttacta tataagaaat ttaggatatg atgctagaaa tcatatggat gctaattatt
    33361 tagtaatgcc tgtttttata gctcgtgatg caggtttagg agatatagga agaaatgcta
    33421 tacttacaaa taaagattat ggctctagac ttagattagg tgttgttact acagatattc
    33481 ctcttttaga agatgaatat gttgattttg gactagaaga tttttgtaag gtctgtaaaa
    33541 aatgctcttt taactgccct tctcattcgc tttctaatga tattaaaatt ggtgatgatg
    33601 gaaaatataa ttgggtaatt gaacatgaga catgctatat taaatggaga tatttaggta
    33661 cagattgtgg tatgtgtata tctgcttgcc catttagcca aaatctcgaa actataaaga
    33721 atactacttc atttaagggt aataatgagc ttattcaaaa agctttagat gagtatactt
    33781 caaaatttgg taaaagaata tttatacctg gaaatccatc ttggttgaaa taattttatt
    33841 taataaagaa tctgtactag aatttatcat aggacaggtt ctttattaaa tatataaatt
    33901 tttaaatact ttttctcgtt tataacaatt cagcaatgtt tttagtctat aattgacatt
    33961 ataaaatatt aaactaaaag gagtaactgt atggaaaaaa tattagtagt agaagatgat
    34021 tctatattaa ataaaactct atcatataac ctaattgaag atggatatat aattacttca
    34081 aaatttacag ctaaatctgc actaaagtct atttttgaat gtgaatttga tttaataatt
    34141 ttggatatta atttaccaga taaaagtggg tttgaattat gtaatgaaat aaaggaaaat
    34201 tacaatatac caataatttt tttaactgct aatgatatgg aatgtgatat gattaaaggc
    34261 tatgaacttg gagctttgga ttatataaca aaaccattta atatcaattt atttaaacag
    34321 aaagttaaag cttttttaaa tcatcttaca acaaaaacaa aacaagatta ctatagggat
    34381 ggatatcttg aaattaactt ttctgagtta tctgccaata taaatggaaa tcaaattata
    34441 tttacaccat tagaatatcg tactttaaaa ctgcttactg aaaatcctaa aagtatttta
    34501 acaaggaaag tacttttaga aaaactctgg gatatagatg ctaactttgt agatgaacat
    34561 actttaacat ctgttataag taggattaga agcaaaattg aaaaagacaa tcttaagtat
    34621 atcaaaacgg tatatggtat gggatatatg tggttaggtg aaagaaatga attttaaaaa
    34681 attttctttt agttctattt atttaataat tgctgttggt ataattttta cagctatttt
    34741 atttaatatt tcaatttatc taataactga tgatttaaaa ctaattgctc tattattaat
    34801 atatacaata gtactttgtg gatttatagc attaataata tttttacttc ataaaaaaat
    34861 agttatattt tgttctaaaa tatgtcacac tttagatagt atgatgaata gtgaagacaa
    34921 tattgaaatt gaattagaag aagaaactat tttatcacgt atcaatcaca gattaattag
    34981 aatgtatgaa gttattcaag aaaatcgcaa tagtattgct atagagaaag ctgatttaca
    35041 agaactagtt tctgatctat ctcatcaaat taaaactcct attgcaaatt taaaaatgat
    35101 taatactact ctactaaatc aaacatttaa aacagaaatt caaaaggatt ttttaatgga
    35161 tatggaaaac caattagata aattagattt tttaatgcaa tctatggtaa aaacctccag
    35221 actagagaca ggtataatca cactatctaa aaagaaaaat tcaatttatg aaacaattgc
    35281 tacagcactt agtggaattt tgtttgatgc tgaaaaaaag aatattgaag ttacagttga
    35341 ttgtgaccca aatttatata taattcatga caagaaatgg acttctgaag ctatttttaa
    35401 tatattagat aatgctgtaa aatacacaaa ttctaatggt aagatacgtg tagctacaga
    35461 atgttgggtt atgtatacaa aaatagatat tattgataat ggaaaaggaa tatcagaaag
    35521 tcatcaagca gaaatcttta aaagatttta tcgtgaagaa gatgtacatg acatagaagg
    35581 tattggaata ggtctatatc tttcaagaaa aattatatct cttcaaggtg gctatattaa
    35641 agttacatca gatattggaa aagggtcgac tttttctatc ttcttaccta ataaataaat
    35701 ttttaaatat tttatttgta atgattctgc aacattttga ttttatactg tatttacaag
    35761 atgttaagga ggtattatta tgagtatttt acaagtaatt aatttaaaga aatattatgg
    35821 acaaagttca aacattacaa aagctcttga tggtgtaact ctatctattg aaaaaggcga
    35881 atttatttct attgtaggta catctggtag tggcaaatcc acattattaa atatgatggg
    35941 aggattagat aatcctacat ctggaaaggt tattataaag ggtaaggaat tatctactat
    36001 gactgatgaa cagcttacaa tctttagacg cagaaatata ggttttatat ttcaaaatta
    36061 taatcttgtt cctttactta atgtatatga aaatattatc ttaccaatag gattggatgg
    36121 agagaccata gataaggatt ttataaatga aattataaat atgctttctc ttgaagaaaa
    36181 attatatgat atgccaagta atctttctgg tggtcaacag caacgtgttg ctatagctag
    36241 agcacttgta acaaaaccag ctattatact agctgatgaa cccacaggta gccttgatag
    36301 tagaactagt tcagatgtac tgagtttact taaagtgaca agtaaaaaat tcaaccaaac
    36361 aattgtaatg attacacaca ataatgaaat tgcacaacta actgatagaa ttattcgtat
    36421 tgaagatgga aaaattgtta actaggaggt gtagtatatg tttagaaata ataatcgcac
    36481 atttataaga aaaatagctt taaatgattt aaaaattaat aaacttaaaa catatctttc
    36541 aggtatcatt ataatgattt ctacatgttt attattaaca gtgaccttag tttcatataa
    36601 tgcttctgta gatttagtta atgcatctcc atatcatgct atatataaat ctgtagatga
    36661 aaaagcaaaa aatatactat ataaagataa aaaccttagt ggcgtaggaa cctacaaatt
    36721 aattggaagt aataaaaaga cagactgtat tatgtcgatt gtttatgcag atgatacagc
    36781 aattaaactt atgaattttc aaccattgaa aggcaaacta cctacaaata aaaatgaaat
    36841 tgctatttca gaaaaatatt tacaggaatt tggtttagat aaagatattg gagatagcat
    36901 taaattaaat tattatgatg atattacgaa taaagaagtt caatgtgatt ttattattgt
    36961 tggatttcta gaaaattatt atcaagataa tgccaagcaa tactatacag tcgtttcaaa
    37021 tgattatttt aagaatatag cttctacacc tttacaaaat aaaagtaata cttttgacga
    37081 gtcaagacct gatactgtag atgtattagt taaactatca gaagaaaata cagagaaaga
    37141 ttcagcttca attaagacac agttaaagaa aattggttta tctttaggta ttaagaaata
    37201 taatatatac ttaaatgata actatattga atcaaattta attgatggtg agcagattat
    37261 tacagtttta gtagttggaa tagttgttct tttttctagc gtttttgtta tatatagtat
    37321 tttttatatt tcagttgtta attcagttca gatgtatgct aagttgaaat cgttaggtat
    37381 gacttctttt cagttaaaaa agactatttc tctacaagga aatattttgt caattatttt
    37441 tattccatta ggggctatag cttcttgtat aattgcttac ataattcaac cattagcatg
    37501 gcaaatgata gctgatttat ttataatttt aattttatca ctggtaatgt ttttaactgt
    37561 tagaatttct ttatttaaac cagctagaat tatttctaaa atatctgcta ttgaagccat
    37621 gcaatataca gaaactaaat ttagaaaaca agcaaaaaat tttagttata taaatataaa
    37681 aaatctagca ttaaagaata tcgagtcaaa tagaaagaaa aatttgattg cattaatatc
    37741 gctaagcatt agtggagttt tatttatttc aatcgctaat cttgctaatt caatagattt
    37801 caaaaaacaa ctatctcaac aatttgtcta taacgaagat tatataatta gcattaagag
    37861 tgataactta tatgagcata ttacagaaat ccaacaaaat aatccactaa cagattctct
    37921 taagaatgaa attaaatcta ttcatggagt aaaaaaatta attgaatcca aatcaattaa
    37981 aagtaatatt atagaacctt acatcaaaga tagagacgaa aaacaattta ttactctaat
    38041 taaaggtatt acgcctgaac tttcaaaatc acttgaagaa tatattgtca gtggaaaaat
    38101 agactataca catttagatt cagatagttt aattataaat aaatatagaa caaaatctta
    38161 tggattaaat ttaaaagttg gagatacagt tacatttaat atctttagtg gaaatgatat
    38221 tattaaaaag aaaatgaaaa ttataggaat cattgacaat tccaatacag gaggtatgtt
    38281 ttttgcttca gacaaaactt tagaatctct aacaccatat aatacaaatt tagatttttc
    38341 tattttaata gataaaaagc attctaaaga agttgaagaa aagttgttaa gtataattca
    38401 gaaaaattca aatttaatgc tttattcata tgaagatgat tatagaatga ttactagagc
    38461 atttcaatat ataattatag cttcatacat ttttgttggt ttaatttctt gctttggtat
    38521 actaaatatg accaataccc taataaacag cgttttaatt agaaaaaaag agtttgcctt
    38581 acttcaagcc gttggaatga caagaaagca actgcgaaat atgctttata gagaaggtct
    38641 aaacataagt atcaaagcta tatgtacttc aagtatacta ggttattttg gttcaaatct
    38701 actttgcact tttataaaag atgtaattag attagacttt attaatttta aattttctat
    38761 atttactatt ttaatttttt cattcgtatt aattggtata caagttttag tgactgaatt
    38821 acttataaga aatatagaaa aaaaatcact tacagaacgc ttacgttctc agtaagaatg
    38881 tatataaaaa ataaccattt tttatacaag ttttaacata attttttaaa agctaaaagg
    38941 atagaaaatt ttgactttac ataattttct gtccctctac ttgtttttat tttatacaga
    39001 tacacttcct tatatctcca agcaacatca ccagagacat aaacaagcat attccttatt
    39061 ttctaaatat gtcaattgca tgacttgtaa ctcctactaa atcttcacgt tcacaagtag
    39121 caaggtgata ttttaaatat aacgcaaatg catcatcatc catagagtct actgcttcac
    39181 gcatatacaa agccaaacca tctgatgcga cataatgcag tctagttaca ttaaatgcag
    39241 acatcaaatc atcaatgttt tctttacgta caagctcaaa caaatccttt ggttcagact
    39301 tagcagcaaa tgtctgtgga tcaatcaaac ctttttcaat atattcagat acattaatat
    39361 ttcctctatg gaacccctcg tcaatgagac agccatcaga tataacgtat gcagcaaaaa
    39421 ccacaccacc aggttttgtt acacgaattg cctcatgtaa agcttgttgt ttatcttctt
    39481 tgttataaag atgatatagt ggtcctaaca gtaacgtaat atcatattta ttatctggga
    39541 aaacagataa atccatagca ttaccctgag taatgcttat attttcattt gattgagtat
    39601 ttttatggaa aacctctata ttatgttcga ctaattccac tgcgtcaaca tcatatccct
    39661 gacgtgccag tgtgtgagaa taacgaccag ttgcagcgcc aatttcaagc acacaatcac
    39721 ctgattttat gtacttatca atgtaatgca tagtagtgag aaattccacc attccatgtt
    39781 ttaatgcaag tcgactatct tcatcataag tattataaaa gtcaattaag tattgttttg
    39841 tttccataat ctcacctctt ctatattagt taataaacat caaatagaca aattccaatc
    39901 tggtttttct tgtcttataa aagctgttaa tatatttttc aacatttttt cagcactata
    39961 tctatctaat gttaattttt ttgttgctgt taagacagct atgccatgag tataaagaaa
    40021 taaatctaaa aatattactt ttgcacattc caactctatt ccaatgcttt ccgaaaaaag
    40081 tcttgccttt ttttcattgt caatttcttt ataaaaatca ttcgcctctt tcatcatcaa
    40141 atctaaatca tttataaata acaacttaaa taaatacgtt tcttcttgag caaattcaat
    40201 gtatgaaagc ggaagcagta aataagggct tacactttta gaattacgat aattaacaac
    40261 atactgatta taatattcat atgcaaaagc aagaaattca ttttttaatt cattcatgtt
    40321 ttcgtaacaa gtaaaaattg gtcgagtgga acactgtaat ttacttgcaa tactcctcgc
    40381 atttacagct tcaaatccca cttctcttgt aatatccaag actgtattca aaatcatttc
    40441 ttttgttatc tttactttag gtggcatata tgttcacctc tccattaaat ttttataaaa
    40501 cacatatgtt atataactat agtaacatat caagaaatta tgtcaagaaa aagctttgca
    40561 tttttttgca attcctaaaa cttataaata aaacatgagt tgcaattaat ctattaatag
    40621 ataacagcaa ctcatatttt cctttaaata ctcttctttt tgtctaatat ttcatataca
    40681 caatcttcta ttgaattaat atctttttct tttctttttt tagaatcaat taaaatgaaa
    40741 tacagtataa aacctacagc aagtccccat gttccgcctt tagcagcaat tacacctccc
    40801 ataagtccaa tcaatccctt ctcaacacct gtattacata gctccattga tagctttgta
    40861 cataaatatc cttgaactaa aagtgttact gacaatgcta atggtataag aggtgtagca
    40921 atctgcgcaa ttggataaaa acagataaaa attgctgaaa cccatactaa tgtacccatg
    40981 ccactaaaaa cagactgcat ggcttccttt ccaaccttgt acctttgagc aacacttcct
    41041 gtaagtgtcg cagctaatgg accgcacatt ggtatatatg gagaaaagat tgccataaaa
    41101 atatttctta tcccacttat aacattagac ctatttgaat tgaaatctat atattcatct
    41161 tttctatttt cactttcaga aagtactaag ttttcacctg ttataaaatc accaaaggca
    41221 attatgtata tcataaaagc cattggtatc ccttgtataa ataacattac acttggaaaa
    41281 cctattccaa aaggagatac tgtatttatc agatttttaa aatctactat gtaaactaaa
    41341 ttgtcaaaac taaatctagg aatttctatt tctttactaa atatacctac tattaaactc
    41401 actaaaatag ctgatactac accatattct cctaaatgaa ataaaaattt attcttaact
    41461 tttagagtat caaatctttc agaaaacatt acaaaacaag caaccaaaat tcctactgtt
    41521 atagatatag gataagaatc aaatctccct ttaggattaa actctgatat tatagtcgat
    41581 ataccgacac ccattagtat tccaccttta atagaatttg gtatcttact aactaacttt
    41641 ccagctacac cagttatacc cattaaaata aatattattc ctaaatctat ttgtaaagct
    41701 gttaatgctt gtattctttc aatgcccatg ctgaacttta acaaatatgc agttgtaaga
    41761 gttatagaag ctgtaatcca gccaggtaca actgggtctc ccataaaaac atgcaaacag
    41821 tataatattg agttaataat agccatactc catgccactt catagggtaa tcccaaaatt
    41881 tgtgtactaa ggccaattgt gcctaaactt gtcatacttg atattatacc ttgaatagct
    41941 tctggtttct caaatctgta atgtataaat ggaattctta tcttaaacat tccaaatttt
    42001 atatgaggtt gttcccttct agcgagtctt tccataactt cactccacct ttcaataact
    42061 cttataagtt tacaacttta taattgttta taaattgtca atgtcattaa aattagtata
    42121 taacatgcaa attatataaa caataaatta aatctataaa attgatagat tttgtgcata
    42181 tatagaaaaa ccgcttcatt tttagttact taaatcactt aatattagtt atttaaatca
    42241 cttgataaaa gttttttata aaattcaata gttctattcc atgattcaaa ctgtcctttt
    42301 gaatttcctt ctacagttcc acctgaagaa aaaatccctc cactcatact catacataaa
    42361 gtctccttta gaggcataat atatggaatt ggtaacggat gacccatatt attgtatgtt
    42421 aataattcta cgttatatgg ataattattt tcttcaaaga tttctaatgc ctctaaacaa
    42481 gcttcataac tattccatat gttatcttct tttccagcaa tcataagtat atctgctttg
    42541 gaattttcta tttttattct agcctctttt ctattctctg ctcttttaag acttttctta
    42601 taagtactat taaatccaaa tggaatacct tttgatatat tttttttctg ttcttcaaag
    42661 aatatatcat tgtctacttt gatatatgga aatggctctc ctttatatga ccatgatgaa
    42721 gtatcatttc cactcatcaa tccattaaga gcttggaaac aataaatgtg aggttgtgat
    42781 actacaacct ttttaatttg ttcatatctt gaagcaagta aaaaggccaa ttctccaccc
    42841 tttgatgtcc catgtacaaa tatttcttta gtactggtaa tttcattttc actaatccac
    42901 tcaaatactt tttcaaaata ttctaatgga acctcttcta atttatctgg taacccttct
    42961 aaagcaaagt atgcaactgt tagtacattg aatcctctag atgcaagtgg agctgctaaa
    43021 aggtctagag cgtctaaatt tccatcagaa ccacctagca ttaaaattgt tttattatta
    43081 gaattttgtt tataaaataa ttttcctata aactcatcag atatgtattt tgtggtaata
    43141 tcttctgatt taaatattct ctttaattca acttcttttc tttcagtaga agtttcaaat
    43201 atcatattca taattattgg tttatcaata gatatatttt ctgcaaaatt tttaccttca
    43261 gttttagatt tttccagcga ataaattaac cccatacaat tatcagcctt ataagtacct
    43321 tctataggtt caactaaatc taaatctact tctccttttt catttgaaca aaacactcca
    43381 taagatgaaa attcttctcc cttgcaccat ggaaattcca ttttcacact tattttaagt
    43441 ttagtatttg gaattaagtc tgaaaccata atcttaagtc tttcatccac aaatgattct
    43501 acattgtata catttacttt cataatctat ttctccttat aataattatt tatgctttat
    43561 atataaaaca actttatcta atacaacata aataaatttt taaattaaga caccatattg
    43621 actatttcat tttatcaaca tatttcattt ttgtcaatat attgtctttt ttagtaattt
    43681 aaaattatgt agtctatatg aaatccagtt gatttttggc taattaaaga taggaatgct
    43741 attaggttta aaatatatct attcaaaata aaaaaccact cattttaaga atggttttta
    43801 atataaacta tttgttatta ataaggatat ttttttattt aaatttatat aattgaacat
    43861 ctaatattta atgtttaata aaattatgca tttgcaaaag tatatatggc attaactctt
    43921 gctacatata aattctcatc actacatcca atatatctag catatttttc aatcttttct
    43981 tttgcatttt caacatcatt ttcatcgctc tccattaact catttatata aatatccatt
    44041 aatgacgctt tatattcttt tatatcatag cctaaaaatt cagtcaatct ctcaatttcc
    44101 ttactaaaat ctccatcttc ttttaccaaa aattcgtata catcttccct atatggattt
    44161 aaagttatta tttgttttac tactttgcat tcgtcagcct tcccaataaa accatcttta
    44221 taattattaa ataaggcatt agattttttt atagattctt catcaatata agaatgtacc
    44281 gcagttatac gattttctat aagggcatct acaacagcat aatgaatttt aaaacaatca
    44341 ttgtataaat aattgctcat tttctttatt gtcgattcta tttctaagaa gctagcgtgc
    44401 ccattttttt tatttttatt tggtataata aaagttttat tcaagttatt aaaaagtctc
    44461 ttaaaatcta aatatttttc acaataaacg cttttaaagg tatcgatatc tatagttata
    44521 attccaaaac ttactatagt ctcaattcct ttcttaaaag actcttctat atatttttct
    44581 cctaaactta aacctctatc agatatttgc tttagatttt gaaaactatt taaacaatct
    44641 ctctcaaagt ttttagatat ttctaaagca tataatttaa agattttcct tatcttatta
    44701 taattatttt ttgtatcatc aaatctgata atagctccat ttacatcaaa agtaatcatt
    44761 gagttttaac ctccccatta gtttcatcaa taaaataaat ttaatataaa ctttataatc
    44821 ataatattaa acttatttat actataatta ttaatttgat gataaaagct attttccttc
    44881 taaataaaaa atttgtctaa agtttctaaa aaatttagac aaatcttagg ttattattct
    44941 ttaaattatt tttaaattat tccaaataca ccaaataata ttggtataaa tatagctggt
    45001 aacaaattag ctacttttat attcgttaat ttaagtacat ttagaccaag tcctataatt
    45061 aataaacttc cagctgctgt catttctgtg actacaggat cacttaaaaa tccatttaaa
    45121 aatgatgctc ccacacatat aataccttca tacaaaaata cagatattgc agaaaacatt
    45181 acacctatac ctaaagaagc agtaaatata attgaagcta ttccatctat aacagatttt
    45241 acaaataaag tatcatgact tcctgtaaga ccactttcta atgaaccaac tactgccatg
    45301 gctcctatac agaataataa actagatgat ataaaaccct ctgctataga atctttttta
    45361 tttcctttag aaaatttact ttgtaaaaaa gcacccaact tatttaccca tttgtcaata
    45421 tctattaatt ctcctatcag agcacctaca gctacggata ttattgtcac taatgtgttt
    45481 tttccttcta gtgctcctga tataccaatg tacaatacac ataaggcaat tccattcatt
    45541 atagtatcac ttactctttg aggtaatcct ccttttatta taagacctac tatacatcca
    45601 cctgcaattg ctagactatt tgctattact ccaaacattt tttctcccct cgattagcta
    45661 attgaaattt atcctattat taaaacctaa gcatctatac tttgtattgg atattttaaa
    45721 tattccaaaa attcttcaaa accttttcta gatatattta cagttttagt atttatgtta
    45781 ggatgaaaat ttaagtaatc ttctttaatt acatctttat ctatgtaaag ctcaactttt
    45841 ctttctgtat catttattaa tgaaaaagga tttacacttc caggaagaag tttaagtaca
    45901 ttgtatagtc tttcaggtga agcaaacgat aatctagaac ttcctatctt atctttaagt
    45961 gagtttaaat caacttgttt ttctccttta actacaataa gataatactt atttcctttg
    46021 gcatttctta aaaataaatt tttacattga cacccctttg ttattccatc taaaatgact
    46081 aattgctctg ctgtatatac agcttcatgc tctactacat catattcaac atttaattta
    46141 tccaaaatat catatatttt agtttcattc atgctagatt cattttcatc ttgttctttt
    46201 ttattattta aatcactcat acttttctcc ttaaacactt catcataata tcattgtgag
    46261 gtattattct actatagcta ttgctcttac tggagctcca tcagcatcat tatacttaaa
    46321 tggtgctgct ataaatagaa atttttctgg aacattttct aaatttgtaa gattttctat
    46381 gattattttt cctttttcaa ataaaatatg atgcacttca aatactgatg tgttatatct
    46441 atccactgat agcatatcta ttcctatgcc ttttatatta gtattagcta tatagtttgc
    46501 agcttctttt gttaatgtag gataacctac atagtactgc tttttatccc aaaattttga
    46561 ccaacctgat ttaaatatta taaaatcact attttttatt tcatcttcat atttttttag
    46621 atattctaat tctatattct catttccctt ttcaagttcc aatacataag ctttcccaac
    46681 aaaattttct atatccaact tatctagggt ctcaccttta gtatacatat gtcttggtga
    46741 atccatatga gttccattgt gagaaaatac actaattaat gtttcttgat aaccattttc
    46801 ttcaataata gctactttct tgatatctgg tctattaggc tctgcatata caggcatgtc
    46861 attatgtgtc acatgagtta aatcaaaaac cttcatcttt atataccccc tatgttttta
    46921 ttttctatta tatacctaaa ttcattttat ttctatcaga ttttattcca ttatatacca
    46981 tactactata tattttttat aaacctcaag ggatttaccg aaaaatcttg gaaagaatta
    47041 tcagtaaata taacatcttc acccaaaaaa cgtttatacc ttccttcttc tttctttaaa
    47101 gtaattctct gtttttgtga atgattatta aaaaccatta tcttaagagg aggttttttt
    47161 ctattagtct gcactctttt taacatgtat tttatgtgca tatcagcttc aggaaatgaa
    47221 tatccacaaa atattaaaag gtcagtatct cttaaagttt tttctgtttc attccaaaca
    47281 ttacttaaaa atatgtttga catattctta aaatatgttg gtggcactat aataggttct
    47341 gttaactcac cacactctaa acattttgtc tctgaactat tttcaataag cttcatgaca
    47401 ccaccttcat gtggtgtaag agttacacta ttacaaatgg ggcaataaag ccaattcaat
    47461 gacccatgta tcttatataa tttgattaat ggttcttttg gttttctcca atttaactcc
    47521 tctttaaaat ttgtaaactc cacaccataa tcaagcataa ttggcaaatc attcttatct
    47581 cttaatccag taactgtatt gtctatatgt atatcataat ttgcactaat aaaagtagta
    47641 tcaagaagca aatcattttc caaaagattt tcaactaaaa gtttatgata tttattatct
    47701 gttctaggtg tattatgaat tgcttcagcc attagcagta ttaaatattg tcttaaaaaa
    47761 cgtatactat cacttctgtt atttagattt tctagtccaa aatttctaaa tgcttctttt
    47821 ctttgttctg ctaagtctaa aagacctaaa acttcttcaa aggtaggaaa atttgcatta
    47881 tctatattat cttttactat atcaatattg aacatctgtt taaagaaaat atacaattct
    47941 ctattcattt tggtattata atttgtggga tttagttttt taaaatattc gctaaaaagt
    48001 tcattttgaa taggtagatt ctctgctgct gaagctcctg ctcctaaaaa tattgctatt
    48061 ttcatatagt tcctcctaat ataaatatta ctcctatttt tatatccatt aaatatccat
    48121 atcatttatt ttatgataca acaataaagc tgtcttttac tagaaaaaat cctattacaa
    48181 gacagcctta tattatatta aatctttaat aattcgtaaa tagaaagagc atatatttga
    48241 gtagctttta taatatcctc tactattaag tattcattag cttggtgttc tgtttctggt
    48301 tttcctggaa aaatagctcc aaatgcaaca caattatcta aagctcttgc gtatgttgct
    48361 cctccagatg aaagtggtgt tccatcaaga ccagtttctt cttcatatac ttttctaagt
    48421 gtcttaacaa gaagagtatc ttctggaaca tagattgaat ccaagaaatc atattcttca
    48481 taatttaaat tgtatttatc tgtcattttc tttaactctt ttacaaaatc atcctttttg
    48541 tatgttactg gaatccttac atctattcct gcaaattctt tttcgttatc aatagttact
    48601 ctacctatat ttactgttag tttacctgat acatcatcct tgcaattagg aattatattg
    48661 tttccattag catcttcacc aataacttct gctaaaaact taattatatt agaatctata
    48721 cctatattat taagagctat acaaagtcta gccactgcat ttataccagt atcacttgct
    48781 gctgaatgga cactttttcc aattatacag attttattac cttcaacagt atactcaaaa
    48841 tttaatttat caagttcttt ttttaattta tcagagtatt ttccaatata gattgcttta
    48901 cctggaactg catttaaagc ctttccaact gacaactcta tatcactttt gctatcacaa
    48961 gtcaaatgaa cttgtaaaag acctttctct gcatttgtta ttgggaatct agaatctgga
    49021 gtaaatccat agtttggtat ttcttcatta ttttctttat atttatttat acatctccaa
    49081 agattttctt catcagcccc aaaaatgaat cttatcctct tattaaagtc tacatttaaa
    49141 tccataagag ctttcactgc ataaattgct gatattgtag gtcctttatc atcttgtgtt
    49201 cctcttccat acaatttacc atcttctaaa acagcttcaa atggagggta attccagctt
    49261 tctaaatcac cttctggaac cacatcaacg tgaccaagta tacctatcat tttttcacct
    49321 tgtcctattt ctgcatatcc ataatatcca tctttgtata ctgtcttaaa tcctaaagat
    49381 ttacataagt ctaatatacc tcttaaagct ttatctacat tttcaccaaa tgggcaattt
    49441 tcagttgctt cagatataac actaggtatt tttacacttt cttgtattga agaaatcatt
    49501 tcatcttgta gagaatttac tttttcttta atttgctctt tcatattatc acccttcaat
    49561 tttcatcata atatactaat aaatattttg acctttttaa aaaaaaatat gcttattttt
    49621 tcagtttttt tataaattca atcgctttta aagtccccat ttttgaatgg tcaaatgtaa
    49681 gtccaccttg aaaataaaca ttataaggag gtcttattgg agcatctgca cttaattcta
    49741 tagaagaacc ttgtataaat gctcctgctg ccattatgac ttcactttcg tatccaggca
    49801 tgtcccaagg aacaggtttt acataagaat cgactggtgc agcttcttgt ataccttcac
    49861 aaaaacttat tacttcatca gcattattaa gtctaataca ttgaataata tcacttctta
    49921 agtcatcata ttttggtaat acatcatatc ctaatttttc aaaagctctt gagcaaaaga
    49981 tagctcccat tacagcctga gatactatat aaggcgccaa gaaaaaaccc tgaagtacat
    50041 ttctagttgt tccaaaagta agaccacatt cttttcctat tccaggagaa gtcattctat
    50101 aagatataag ctcaatcaaa tcttttctac cagctatata tccacctgtc aatgcaagcc
    50161 caccaccagg attttttatt aaagagccag ccataacatc tgctccaaca tcagtaggct
    50221 ctttggtatc taaaaactca ccataacaat tatcaaccat tactatagct tctggtttaa
    50281 cagactttat tacttctata gcttctttta tatcactaat tgaaagtgat tttctccaag
    50341 aatatccttt tgacctttgt atcataacga gtttagttct atcatttatt ttgtttttaa
    50401 ttccttctaa atctaagttt ccatcctcta aaaaatctac atcatcatat gtaacaccat
    50461 actctttcaa tgaacctttt tcttctctta tgcctataac tccctctaaa gtatcatatg
    50521 gtctaccagt aacagataaa atttcatctc ctggtctaac tataccttgt atacataaag
    50581 taagtgcatg tgtaccattt acaattattg gtcttacaag agcatcttct gtattaaata
    50641 ctttagaata aatttcctct attttttcac gaccaatatc attatatcca tatcctgtag
    50701 tccagttaaa gtgcatatca cttagtttag attcttgcat agcttttaaa actttatatt
    50761 gattatattc tcttatttct tttatttctt caaacttatc ttttatttct tccattattt
    50821 cttgcgatag tttaaaagta tcatcatcta ttccatagta gtcttttaat aattcttttg
    50881 tctcattaag cattttatca cctgtctctt tattatttta tttaaacatg taaaggagac
    50941 tattttacta aaacaaataa attatagttt ataagttagt ataagtctcc tctcaatcat
    51001 cttaaattaa attaattatc tatctgttgt tattattatt gttgttttgg ttttgatttg
    51061 ttagattaat atactttcca ggttgtatag ttgatacagc atgtttataa atcatgtttt
    51121 gttgtctatt atccccttct attaatatta tataactatc aaaccccttt acaagccctt
    51181 taacttgcac tccattaact aaatatatag taacaggtat cctttctttc cttgcattat
    51241 ttaaaaacaa atcttgtaaa tttaaaactg tatttttcat tagatttgta ccctcccaag
    51301 attaaattga acttctaaaa tattcctacc ttatattcta tataagttat gacaaatttt
    51361 caatattaag ttgtatactt aaaaatttat atattatttt atagaatctt taatatataa
    51421 gattatttca ttttttaatt cgtctatatt ttcatattgg tctaaatcaa accatcttgc
    51481 tgttttatat cttttaaacc atgttatctg acgttttgca taccttctag aatctctttt
    51541 aattatttca atagcttctt cgtatgtata ttctccatcc agatacttca atatctcttt
    51601 ataacctata ccttgcattg atatcatatc ttttttaaat cccatactta aaagtttttt
    51661 tacctcttca accaatccat ttttaatcat aatatcaact ctcatattta ttctttgata
    51721 caaatgttct ctatctctat ttagaactat tattattggt tgatattctt cattaaattt
    51781 taaatcactt gaaaaatcat tcattttctt accacttaga caaacttcaa gagctcttat
    51841 gaccctttta gtattgtttt tatggattct attagcagat tcttcatcaa gttcttttaa
    51901 cttattatgc atgtaatcta ttcccttttc agcaagctgt ttttgaagtt cttctcttaa
    51961 ttcattattg gcatcagatt gtgcaaaatc catattatat attaaagagt ttaaataaag
    52021 tcctgtgcct ccagttacta aaacacattt tcccttttca tttatttcat gtatatattt
    52081 ttttgctctt tgttgaaact ctgaaactga aaatggatag tcaggcttaa cttcatctat
    52141 aaggtaatgc ttcactcctt gcatttcttc ctctgtaact tttgcacttc caatatccat
    52201 atattcatat atttgcatag aatcagctga aattatctct gcatctatat cttttgctaa
    52261 ttttattgac aaatctgttt ttccaacagc agttggacct gtaagtatta taagtggtat
    52321 ttttttcata acattccttt ccatatattt taaatatcca aatattttac ataattcttt
    52381 taaacatttt ttctatttct gtcttagata tttcgaccat tataggtctt ccatgagggc
    52441 atgtaaatgg attctcacac ttttctagct gttccaataa actttttatt tcaatatcgt
    52501 atattttatc atttgctttt atagcagacc tacatgccat agatgcaaat ctctctcctt
    52561 ttaaatcata attacttgta atttcttcta tattatcaat tatttgaagt ataaatttct
    52621 cagtttcagg tactccaaat atagtaggaa cacatcttac cattatatga ttattgccaa
    52681 aaatttctag ttcaaaacca aatttcataa ataactccaa gttattttca atttgaagca
    52741 tatctacatt tgatacttca ataaccactg ggtctaaaag tatttgcata ttgatatctt
    52801 gtctatagaa cttctccata tatctttcat atagtattct ttcatgtgca gcatgttggt
    52861 ccaacaaata catagaatcg tctttgctca aaattatata agtattaaat acaactccaa
    52921 ttacagaata tccatataag gaaaacttgc gttttgaact acacaagtat tcttcttcac
    52981 tgtccttaat actatctcct aagtaataat ttccttcatt tttaattcca tcaacttgaa
    53041 actcttcttg tatattatca tttgcactat cttcacttag ataattaatg ttttttacat
    53101 catcttctac acttgcattt aggacttcac ttactgattg aaattcattt attggttttt
    53161 cacttgataa agtcacaact tcaatgactt catccttatc tttagtaata ttactatttt
    53221 taggagtaga ttctaataaa tcattatttc ttaatttata atctgtactt ttttctctac
    53281 tgttaatagc gattctaggt tgtgtttttt tatcactata agtagcatat tttccaataa
    53341 gatttgagtg tattaattta acctttaaaa aatctctaag ttctatatac acttcttgtt
    53401 ctttttcaaa tttaatttca agtttattgg ggtgtatatt tacatctata catgatgggt
    53461 ctacttctat atttaagaag cacactgcat gtttacctat tggaattata gacttataac
    53521 tttctgttat tgcatctatg attattttac tttttacaaa tcttttattt atatagatat
    53581 gttgtaagtt tttatttgaa cggtatatat tgttattacc tatatatcca ttcattttaa
    53641 agtgattgca cttaaactct acatctatta tattttccgt tatttctttt ccatatatac
    53701 ttctaatagt gtttacaagt tttccatccc ctggagtatt taacatctgt ttattgttat
    53761 ttgtatattt aaattgaatg tttggattac ctattgcaag tttatttatc aaatcactta
    53821 tgtttatagt ttctgcatga gtagatttta aaaatttctg tcttgctggt gtattaaaga
    53881 aaatatcttt tataatgatg gtagttccat tagtagaacc tattggttct tttgatatta
    53941 tttttccgcc ttcaacatat attttagttc ctattatttc atctttagtt ttagtcgtca
    54001 tttctaattt tgatacagct gaaatagatg ccaatgcttc tcctctaaaa ccaagagagt
    54061 ataaatcata caaatcatca atttttttaa ttttactagt agcatgtctc aaaaatgatt
    54121 tttcaacttc acttgaaggt attcctattc cattatctgt aatcttaatt agactttttc
    54181 ctccatcaat tatatctatg gaaattttat ttgcacctgc atcaatggag ttttcaataa
    54241 gttcttttac tacagaagaa ggtctttcta caacttcacc tgctgctatc ttatttatag
    54301 tcaaatcatc taggatgtta attatatttt tcatttttac acctctaaga ttttatttct
    54361 tttgcttttc tttgaagatt ataaagagag tttatagcat ctaatggagt catatttaaa
    54421 atatccagat ttaaaatttc ttctgatagt atatctctat ttacactatc aaaagagatt
    54481 tgagttaagg ctacttcttt tacagaatct tcttgattaa ttttctcttt atcatcattt
    54541 tgcttcatta aagcaacttc atcatttaat gctttaaatt tagaatttaa tttatcataa
    54601 tcctttttta tatgtttgta gtcttttact ataagttcat gttctatttt tagagattca
    54661 tatttattct taaaattact ttgatttacc ttaactaatt cttcgtccaa attactatta
    54721 attatattat tgttttcttt atctccattt attgcaacac tgttgtaaac atgatttttt
    54781 tccaagtctt taagtatata ttttgctctt tctatgactt cgtctggtaa tttagctaat
    54841 ttagcaacat atattccata actcttgtct gctccctgag gaattatttt tcttaaaaat
    54901 attattcctt ccccatcttc tttgactgct attgaataat ttttaacctc tttaaactct
    54961 tcttctaaat cagtaagttc atgataatgt gttgcaaata atgtcttaca tctaatattt
    55021 ttttgaatat attccactat tgaccatgct aaacttatcc catcataggt actagttcca
    55081 cgacctattt catctaatat aactaaactt ctctctgttg cattttttaa tataagggat
    55141 acttcattca tctctaccat aaaagtagat tgaccttgag ataaatcgtc actagctcca
    55201 actcttgtaa atattctatc taatattgga atatcagcag actctgctgg tacaaatgag
    55261 cctatatgtg ccataagagc tattatagca gtttgtctca tatatgttga tttacctgac
    55321 atattaggac cagttattat gttaataatg ttttctccac gatttaaata agtatcattt
    55381 ggaacgaaat tctcctctcc tactatattt tcgacaacag gatgtctacc atttcttata
    55441 tcaagtttat tattttcatt tattgctggt tttacatagt tatttatatg tgcaactgtt
    55501 gcaagggaca caaaaacatc tatattagct atagtcttag ccactttttg tattctatct
    55561 atgtttttat atattgtatc tcttatctct acaaatatct cgtattctaa agatttaatc
    55621 ttttcctctg catgtagtat cttttcttct atttccttta attctggagt tatatatctt
    55681 tctgcattac taagtgtttg ctttcttata tatgtctcat ctagctttgc ctgtttaaag
    55741 ttggcttttg ttatttctat ataatatcca aaaactttat taaatcctat ttttaatgat
    55801 ttaactcctg ttttttctct ttccctgttt tctatttcct ttacaagaaa ggctccattc
    55861 ttagaaatct ctcttagttc cttaagttca tcactaaaat ctgatttaat tatgtttcca
    55921 tcttttatgg ttatcgtagg ttcttctaga atagcttcat ctattaaatt gtatatatca
    55981 tctaatttat ccatttctga aacatactct tttaggattt ttgcatttga taaatttata
    56041 gtatctttta aattaggtaa cttttctatc gaatttttta agtgaattaa ctcttttggt
    56101 gttactctct caaatgctat ctttccacat attctttcta tatcgtatat atttttaagt
    56161 atatcattta ggtcttctct taatataaag tcatctttta tttcttctat tacatctaat
    56221 ctattttcaa ttttagattt atttatcaaa ggttcttcta catattttct taaaagtcta
    56281 cctcccattg ctgtagaagt tttatcaagt acatgtaata atgaaccttt tttcttattc
    56341 cctcttatag tttgagtaag ttctaaatta gttctagtaa acatatctaa aaccatatat
    56401 tctgatgaat tgtatatatt tatattatta atgttagatg ttatttgttt ttgagtattg
    56461 tatatataat tcagaagaat tgataagctt gactttatta gacctttatc atcaaacttt
    56521 agtttttgta gataaacatc tggaaaatat tcttttaata tatttatatc caaatagtta
    56581 tcactaaaac tttcatttat gtatatatta cttacagtag ctatatctct aagcttttct
    56641 ataaagtcta aatcatttat aattatttca gttggatgga tttttgcaat ttcttcaatt
    56701 accttatctt catttaaaca tgttgcatta gtttctcctg tacttatatc aacataagtt
    56761 agacctatat tagtaccatc cttatacaaa gataataaat aattattctt tttattttca
    56821 agtaaatttc catctaaaac tgtaccagga gtgatgactc ttataacctc tcttctaaca
    56881 attccttttg ctgtagatgg gtcttccatt tgttcaccta ttgctacttt atatccattt
    56941 tcaactaatt ttgatatata tgaattggct gagtggaaag gaacaccaca cataggagca
    57001 cgttcttcta aaccacaagc ttttcctgtc aatgctattt caagagcttt tgaagctact
    57061 aatgcatctt caaaaaacat ttcataaaaa tctccaagtc taaaaaacag tatacagtct
    57121 ttatatctat tttttacttc aagatactgt ttcatcatag gagttaattt attcatatct
    57181 atttgcat
//
LOCUS       DAELXX010000010        56305 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.010, whole genome shotgun sequence.
ACCESSION   DAELXX010000010 DAELXX010000000
VERSION     DAELXX010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 56305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56305
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.010"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            594..1403
                     /locus_tag="KPH29_000609"
     CDS             594..1403
                     /locus_tag="KPH29_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG1466249.1"
                     /translation="MKDYYKIGEISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNI
                     SDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEELDLIDKKIAEFISHKEN
                     IIKRLSSIESVIQNTKIDEIEVVYIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRF
                     NILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNS
                     NNKMYMEKVFKFIEENNYKIIADPIEIYKIDIHETGIVEEFVTEIQVPITK"
     gene            1548..1787
                     /locus_tag="KPH29_000610"
     CDS             1548..1787
                     /locus_tag="KPH29_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466250.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDGKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNTDRNWCKDKK"
     gene            complement(1975..2151)
                     /locus_tag="KPH29_000611"
     CDS             complement(1975..2151)
                     /locus_tag="KPH29_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466251.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(2205..2301)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(2656..3330)
                     /locus_tag="KPH29_000612"
     CDS             complement(2656..3330)
                     /locus_tag="KPH29_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBG1466252.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
     gene            complement(3550..4104)
                     /locus_tag="KPH29_000613"
     CDS             complement(3550..4104)
                     /locus_tag="KPH29_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBG1466253.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PVIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIIIKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYHK"
     gene            complement(4182..4547)
                     /locus_tag="KPH29_000614"
     CDS             complement(4182..4547)
                     /locus_tag="KPH29_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1466254.1"
                     /translation="MKIRAEYTCPLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            5012..5797
                     /locus_tag="KPH29_000615"
     CDS             5012..5797
                     /locus_tag="KPH29_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG1466255.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEDGNITSIILKNKSTLIIYSFKNNFDIGEMKNHIKDLDVESISGKKCVIDRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKEHLKEGNVRAYFIRNDDTMISTVSTGMETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDVAGKIYHNIGYKEINEWTILFK"
     gene            complement(6168..7346)
                     /locus_tag="KPH29_000616"
     CDS             complement(6168..7346)
                     /locus_tag="KPH29_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBG1466256.1"
                     /translation="MKTNKISFNKGLYYLNTNANFNFQLNRIIMWDGGRLEDIQKVSS
                     KIVDSESWKTELMYLAQEAEQDGRTENAIAYYRMSEFFMYDGDPDKISVYKKATTLFY
                     NYYSEYFSNGTVQIFHVPYENITLPVMYAKAQGEVKDVILLHGGNDSYFEEFFFPMLY
                     LASQGFDIYLFEGPGQGGVMRLQGKHFTYQWERPVKTILDYFHLENITIVGASLGGFL
                     APRAAAFEKRIERVVAWSVFPNFLDIIVGSQPIKMQNLLRLLLKLKTKWLVNFIIKQK
                     MKSGDSMVVWGLKHGMYAYEASTPYEYFQKIKYYQIDSIAKNINQDILILGATQDHFI
                     DYRTVGKEIDLLNNVRSLTFRLMTEAEQASNHCNCGNSKLVLDTISNWIMMMKIHDRV
                     "
     gene            complement(7449..8213)
                     /locus_tag="KPH29_000617"
     CDS             complement(7449..8213)
                     /locus_tag="KPH29_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1466257.1"
                     /translation="MTTICVMIDHIFLDTEYRQPTIHKHLAKHIIISQSGNLSVTFDK
                     GESLQCKGIIIDSNVSHTVSSKDNKMLVFLIDDTSILAQNISQHQLLNHSFFVIDNLI
                     VERIQNIYTACQVKGLSQSYIDFSKQAFRLLNLNYHISSITDSRIQWALNHILKSTHI
                     HNEIFFEICAEVKLSKSRFSHLFKEQMGVSLNSYLALSKLRKAYELLLKSGDITSAAM
                     DAGFSSPSHFSASSKKYLGITASELIGKCKIYFIRQ"
     gene            complement(8402..9226)
                     /gene="pdaA"
                     /locus_tag="KPH29_000618"
     CDS             complement(8402..9226)
                     /gene="pdaA"
                     /locus_tag="KPH29_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBG1466258.1"
                     /translation="MKKDSLKKYIMIGAFALILFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            9447..12566
                     /locus_tag="KPH29_000619"
     CDS             9447..12566
                     /locus_tag="KPH29_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBG1466259.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIYTY
                     ESLIKNKFEVPIEVFSVCKNLDKNDLEIANEIELVEDRLVNFVEDSFIICHDLSLKKK
                     FFEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKNTLTNCNSKVENRALSDAIDII
                     NIVNCLLVKFNNYEKTTLEPLSFKINSYLKKFNLPTWEWSKFLEEANYDLSNNINIKK
                     EYNIFDSKEEKKKERETLKILNEEEKNYEELLKYKTIWENKEGFTYEYRPGQYELTKT
                     IRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEARINKKRLLISTDTKELQIQLI
                     NKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAYIDDYESQNPTKGEILSLIFLK
                     RLVEGGKYGDIEEINYLVFDNFKEISTHLRNVSCDPNMCRPKKCKKDCLYKNRIEELK
                     EEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLTEKGYDFFSSIINSKSLRYLLQEI
                     YPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIGREDKQKIARSINLIIEEIDSIL
                     KFGNCNEYNNVSNYNLRWELNLQIDEIVGKLKKDGIDTEISYRAYSEKIKLSCEKIIK
                     NLVSIIIIIYRNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDDYARIV
                     EIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSYFKNTLGIDRV
                     KNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISKITEGHTLALFNS
                     KDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCFEGVDIPGDGL
                     VCVTLDKLPNLNPKDPLYFTIMKKYGIDYYTINYPQMTIKVKQAMGRILRSKYDYGCF
                     VIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTYIRRHLNKSRSLILKSVIFDTIKA
                     LNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVYHIDIIKKDMKVKYFDRNYLINLD
                     IFMREEDKN"
     gene            12584..13183
                     /locus_tag="KPH29_000620"
     CDS             12584..13183
                     /locus_tag="KPH29_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HBG1466260.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(13440..15569)
                     /locus_tag="KPH29_000621"
     CDS             complement(13440..15569)
                     /locus_tag="KPH29_000621"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBG1466261.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYNKEDSSYEDFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLVRPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
     gene            15835..16083
                     /locus_tag="KPH29_000622"
     CDS             15835..16083
                     /locus_tag="KPH29_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466262.1"
                     /translation="MMICFAMTFALVLINSVMGVHIGLDYYFLMGSVLTVCLTIGIVL
                     CNARKIFDSKAIHKTRTKTVSTNKKQKSHSRDIKRKIS"
     gene            16249..17640
                     /locus_tag="KPH29_000623"
     CDS             16249..17640
                     /locus_tag="KPH29_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBG1466263.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLAQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKEDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            17755..19224
                     /locus_tag="KPH29_000624"
     CDS             17755..19224
                     /locus_tag="KPH29_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBG1466264.1"
                     /translation="MSNVNKKLVIAIGAVALVAIIFIGIMCMQFKGEKIAKNTYVNGV
                     DIGKLTKSQAKQELAKKYKLENVEFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SSFFGNLSKTISTNFGKKSNLVVVINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLTNIDTNLGTY
                     STIFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNTNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            19289..19882
                     /locus_tag="KPH29_000625"
     CDS             19289..19882
                     /locus_tag="KPH29_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HBG1466265.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            20064..21410
                     /locus_tag="KPH29_000626"
     CDS             20064..21410
                     /locus_tag="KPH29_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG1466266.1"
                     /translation="MPRKILDISLIEDNKSDLTFFNIEEEDGDETLKALKQSYVTTNY
                     KEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKNYITSAINMVNKALEINP
                     KDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKYVNLISSEDFKTFLGRYN
                     HSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDKLGNVKKREGYLDKLKEL
                     DKDNPVFEKNEEEKEDTSKKEEIRKVKRKKSILPYIIVGCVLIGMGIYLIQSKKRIEN
                     LNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEPEREITVADEEDLYYQAL
                     NLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQLAITNKLLGNKDEAIKYYKKYINT
                     YTKNDQYYDDSYYELGMLYYDNGDLKNAQQTFYSLRSEVPDSMYNNSKIKEILSEK"
     gene            21592..21963
                     /locus_tag="KPH29_000627"
     CDS             21592..21963
                     /locus_tag="KPH29_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBG1466267.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            21956..22645
                     /locus_tag="KPH29_000628"
     CDS             21956..22645
                     /locus_tag="KPH29_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBG1466268.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGIESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            23165..24031
                     /locus_tag="KPH29_000629"
     CDS             23165..24031
                     /locus_tag="KPH29_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG1466269.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            24273..25082
                     /locus_tag="KPH29_000630"
     CDS             24273..25082
                     /locus_tag="KPH29_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466270.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKKGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGQGSYTFFVDEGT
                     AMEYIGEFKEDKKNGKGNFVFSNGNEYIGHFEEDMMNGIGNMLYNSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            25225..26289
                     /locus_tag="KPH29_000631"
     CDS             25225..26289
                     /locus_tag="KPH29_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBG1466271.1"
                     /translation="MKRLIALVLVILIVLFYSPELIEEGYDLKEKFFQSEIWNEFLDK
                     ININNNSKKDKKEQNQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKLNQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     KLYPDDREELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HINKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            26317..27036
                     /locus_tag="KPH29_000632"
     CDS             26317..27036
                     /locus_tag="KPH29_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG1466272.1"
                     /translation="MTSWKKKTVYKCLIAVVLFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKDLI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVKNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            27060..27644
                     /locus_tag="KPH29_000633"
     CDS             27060..27644
                     /locus_tag="KPH29_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HBG1466273.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGEAVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGYVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIIDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLENFITEARERVHM"
     gene            27646..28995
                     /gene="pabB"
                     /locus_tag="KPH29_000634"
     CDS             27646..28995
                     /gene="pabB"
                     /locus_tag="KPH29_000634"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HBG1466274.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDTHILSNSPERFIRCVNKRIETRPIKGTRPRGKDKE
                     EDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            28985..29728
                     /locus_tag="KPH29_000635"
     CDS             28985..29728
                     /locus_tag="KPH29_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HBG1466275.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDINYKKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDIPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FIKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            29762..30463
                     /locus_tag="KPH29_000636"
     CDS             29762..30463
                     /locus_tag="KPH29_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466276.1"
                     /translation="MIGCIYEVYNEEMTKIIENQNTLAVYLVGSSKDVDFTLYDVEIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISNIIKDISRDIYLEGPSKMSLEEKSFIKQDIGAKISNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELASKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            30489..31061
                     /gene="folE"
                     /locus_tag="KPH29_000637"
     CDS             30489..31061
                     /gene="folE"
                     /locus_tag="KPH29_000637"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBG1466277.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            31127..31918
                     /gene="folP"
                     /locus_tag="KPH29_000638"
     CDS             31127..31918
                     /gene="folP"
                     /locus_tag="KPH29_000638"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBG1466278.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELEIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVSIEMA
                     MSCGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            31929..32294
                     /gene="folB"
                     /locus_tag="KPH29_000639"
     CDS             31929..32294
                     /gene="folB"
                     /locus_tag="KPH29_000639"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HBG1466279.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRTRDE"
     gene            32287..32793
                     /gene="folK"
                     /locus_tag="KPH29_000640"
     CDS             32287..32793
                     /gene="folK"
                     /locus_tag="KPH29_000640"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBG1466280.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDILFFN
                     DVASTDERLTIPHPRIQDRAFVLIPLMDLNEELIINEKTVKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            32774..33559
                     /gene="aroF"
                     /locus_tag="KPH29_000641"
     CDS             32774..33559
                     /gene="aroF"
                     /locus_tag="KPH29_000641"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBG1466281.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLKILKAVKDEVGMAVITELMDVRD
                     IDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            33814..35604
                     /locus_tag="KPH29_000642"
     CDS             33814..35604
                     /locus_tag="KPH29_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBG1466282.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLDCGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            35622..36788
                     /gene="rpoD"
                     /locus_tag="KPH29_000643"
     CDS             35622..36788
                     /gene="rpoD"
                     /locus_tag="KPH29_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBG1466283.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            36953..37642
                     /locus_tag="KPH29_000644"
     CDS             36953..37642
                     /locus_tag="KPH29_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG1466284.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            37656..38753
                     /locus_tag="KPH29_000645"
     CDS             37656..38753
                     /locus_tag="KPH29_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBG1466285.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSIEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            38815..39387
                     /locus_tag="KPH29_000646"
     CDS             38815..39387
                     /locus_tag="KPH29_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBG1466286.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEVLEEMNIEVDIFRVSDEVAKT
                     LPQYYLINYKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGAPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAYG
                     ILHIAGDNSDIRPNDDAKIGIRDYLKRLNI"
     gene            complement(39447..39914)
                     /locus_tag="KPH29_000647"
     CDS             complement(39447..39914)
                     /locus_tag="KPH29_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBG1466287.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRI"
     gene            complement(40300..40776)
                     /locus_tag="KPH29_000648"
     CDS             complement(40300..40776)
                     /locus_tag="KPH29_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBG1466288.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLRVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            complement(40896..41987)
                     /locus_tag="KPH29_000649"
     CDS             complement(40896..41987)
                     /locus_tag="KPH29_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HBG1466289.1"
                     /translation="MSKIECLFTIFPVLILMYATFYFMPYFVGKKHIYGVSIDQEHKN
                     YNYFIKLDKEFKKLLSLGFIIDFILVFILVFTFNKLELSYFIGIIGFLLYESILYIHT
                     HKKAKKLKSEIYSKLGHIDVDSKLIVDMDFINKKNKIIKKFKIIYLIPILFTLGMSIF
                     IAFNYNKLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTDFCLMVLLYITSIGSIKSR
                     IKIDTNRIEESRVENIKYLNKIGYLFLILMIIMTTQFFTTLLSIKTKLSTAMNITTLL
                     VIIYLIATYINSPNLKFNSSYSPEEDEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINF
                     GNPLGKILYIAIALLLIFSLFSLIKSLLL"
     gene            complement(41980..42369)
                     /locus_tag="KPH29_000650"
     CDS             complement(41980..42369)
                     /locus_tag="KPH29_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG1466290.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            42561..43007
                     /locus_tag="KPH29_000651"
     CDS             42561..43007
                     /locus_tag="KPH29_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466291.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSHK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKEDIDLHEDAKKDNIYKEDEYKKYEEE"
     gene            43271..43996
                     /locus_tag="KPH29_000652"
     CDS             43271..43996
                     /locus_tag="KPH29_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1466292.1"
                     /translation="MKRKEIVMLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSG
                     KEMISSNEYSMILLDVTLPDGTGYELCKYIRGFSQVPIIFLTACDEEVNIVMGLDIGG
                     DDYITKPFRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFL
                     TSVEYKLLLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCE
                     PIYIETVRGIGYKWIGSENSVSI"
     gene            43983..45227
                     /locus_tag="KPH29_000653"
     CDS             43983..45227
                     /locus_tag="KPH29_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1466293.1"
                     /translation="MFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMVVRN
                     NQAIIGTLSSKYPNLESQIVDIITQGKSMKNIDYGQKILSKYNYDKSIKINSEPIISK
                     LVLETIKINIILVCIIFILIFVLVVRYFTSIYNDLSDMTKYVYYSSEGKPFDMKNKNQ
                     EGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLNDLLY
                     NDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHRAML
                     SMKIPMEIKNQKLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITYKEN
                     PLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNGDIY
                     VNSEKGKGTEFHIIFHKMYDSD"
     gene            45307..45984
                     /locus_tag="KPH29_000654"
     CDS             45307..45984
                     /locus_tag="KPH29_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1466294.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLIDREKHLPNQLSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            45985..48546
                     /locus_tag="KPH29_000655"
     CDS             45985..48546
                     /locus_tag="KPH29_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1466295.1"
                     /translation="MNLYTSLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRKNVEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDQNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEGDYIANEKDRTFKVVGIIKKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVRNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINSSI
                     KSIIIAVTILVIICTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTKMSLQIVYNPIIIIVSIIIVLLTIF
                     ISILLPAISASNISPLNVIKNTGEYKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDGIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGESYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGVILVRKSAIEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKVKVKILAITDEERLGFGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNDFNFYDEISSSESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIPMSKLNKENIIDNIRRESI"
     gene            48767..50041
                     /locus_tag="KPH29_000656"
     CDS             48767..50041
                     /locus_tag="KPH29_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG1466296.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSMNDK"
     gene            complement(50202..53243)
                     /locus_tag="KPH29_000657"
     CDS             complement(50202..53243)
                     /locus_tag="KPH29_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HBG1466297.1"
                     /translation="MFKQRISKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNAESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDDGKLNLDTPVVKYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLADNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTTENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYVYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKVDDNNISNSVKEVWKKRSGKGYYLVDEKYTSQSYMFGSVKASFSLSD
                     ETPGYIVNTKIMDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDIANKKIEVNLPQNSAFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGEA
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGNTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKEIA"
     gene            53450..53749
                     /locus_tag="KPH29_000658"
     CDS             53450..53749
                     /locus_tag="KPH29_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBG1466298.1"
                     /translation="MYKNINKKQLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISL
                     QDLINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
     gene            complement(53987..56215)
                     /locus_tag="KPH29_000659"
     CDS             complement(53987..56215)
                     /locus_tag="KPH29_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBG1466299.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
BASE COUNT        22209 a         6192 c         9184 g        18720 t
ORIGIN      
        1 aatattagat aaaattagtt aatgcttaaa ttatagtata ttgaaagatt tcctttttga
       61 atgcaattat atcatatttt ttcataaata gatagattgt gagctgtata gttagtaagg
      121 tagagttttt ttataattaa ttttaagttt aatgattttt caaaggcata attattttat
      181 tcataattaa ctttttatac ataatttaat accacataat taattttagt tattaaaatt
      241 aagtttattc ttgtattttt tgttaaagac tataataaat attcatttac taaatttata
      301 aactattaat ctatagaaat cagttttaat atgaaaaata ttacatagag atggatataa
      361 tagctgtaaa cataaaacta tataaaacta aaatatattt ataaaaaagt tgaagttata
      421 aatactatac ctaacattac aatataaaaa tattttaata attaattatt aggatgttta
      481 ttttcctatc cttgttttaa agattttttt agtatattct aatattatag tattatagag
      541 taataatgtt gatttaaaat aaaaatagga tttcggagga gtttattata taaatgaaag
      601 attactataa aataggagag atatctaaaa tatatggaat aggtagagat tcattaatgt
      661 attatgaaga aataggcatt ctaagaccag ttagagatat aaatggatat agaatgtata
      721 atatatcaga tatatggaaa ttaaatttaa taaaagaatt tagaagcttg aactttccaa
      781 tgaagaaaat aaaagaatac ctagatgaca gaagtataga gtctacgaaa aatattttaa
      841 atgaagaact cgatttaatt gataaaaaaa tagctgaatt tataagccat aaggaaaata
      901 ttataaaaag attgagttcg attgaaagtg taattcaaaa tacaaaaata gatgagatag
      961 aggttgtata tatagaaaaa agaaaagctt tagaattaaa tgctgatata aagagggatg
     1021 aagacttcga ttttttaatt cagaaacttc agaaagaata tgaagataga tttaatatac
     1081 ttggaaacaa caatattggt tctgcctttt ctactgaagc aattaataaa ggtatattta
     1141 atgagtttaa atctgtattt tgttttctgg aaagtaatga gaaagtatat aatatagttt
     1201 ttgatgaagg atattatgta actttaaatt atactgggag taattctaat aataagatgt
     1261 atatggagaa ggtgtttaaa tttatagaag aaaataatta taagataatt gctgacccta
     1321 tagaaatcta taagatagat atacatgaga ctggaattgt agaagagttt gtaacggaaa
     1381 ttcaagttcc tataactaaa tagccatatt tcaatggatt agatggtaaa tagaatgaaa
     1441 aaatgttgat ttgccaccga tttgctactg tgttataaaa tcggtagcaa attatatttt
     1501 aaattataag atagcatgaa aaaataattg tttagggggt tataaatgtg aaatgttcta
     1561 attgtggaag tgtaaatatt gaaaagggta ttttaacaac tggtggcata tatgctgaaa
     1621 ctattggatt aaaatataga ggagatggaa aattagataa agttataaat aaaacttttc
     1681 cagtacaatc tactatatat tcagatttat gtttagattg tggcgaaata gttagaacct
     1741 atataaagga taatactgat aggaattggt gtaaagataa gaaatagaat aatttttgaa
     1801 gtatattaat atttgaagtt gaattttatt aaagatacaa gtgaaaaagt ataaatggga
     1861 agaaattgtt atatggaaga aaactttgca tataggggag attagttttt taatataata
     1921 acatttttaa atgtagataa aactaaaaag ggttctctag aaaacaattt ctatttaatt
     1981 atacttactt accaatacca tcacttgatg taaacaagat tctactacat tttctccgta
     2041 ctctagtata aaatcatcta gctgtgtatc tataaaatca caataatcat ataaactact
     2101 aatataagca gttaacatgt gtgtacattt atccatttca tctctaaaca tatatattac
     2161 tatcccccta aaaaactttc ataaaacaaa aagaacaata tctttttggc aactggctga
     2221 cataactcat agtttatttt ataagcgtta gtccctatag ctttgcgtca ctaaatttct
     2281 ctagttttgc cgatttagtt ttattctaca actaaaataa tacactagga taagttattg
     2341 ttcaacatac ttgtcagaaa attgaaatat ttaacagtat cttaacaata tgaaatctat
     2401 ttttcactca agaaacaaac tatacatgta gaatatttaa taaaaattat atttagtttt
     2461 gtattgaagt ttttgtatta aaaagtttag ttcacaagag ttacttggct agataataga
     2521 tgagcaaaaa tatcttgaaa aaatatatat gaatggcaaa aaacaaggga tgaattttat
     2581 atagactcat cccgtaatta tttatatatt gaccttgatt aaatatatta ttatttttaa
     2641 gcttcaattt ttatattata tttctaaagt ttctataaga ggattatcgc taaataattt
     2701 taataaattt gtttttttat atcctttagg taacttagta tatataacta cttctaacca
     2761 atccccatca aggcgtttaa tattcatgtt tataatttca atattagatt ttttaaattt
     2821 accttcaata tattctatgg aatcagtgct gttagaaatt ttaagagaaa cttgctctgc
     2881 tgtagggagt ttcatccact tcctatctct atgaagtgtt atctgtacaa taacaataat
     2941 gatacttgaa acaataccaa ctaagtataa tccagagcca atggcaagac ctgtaccggc
     3001 tgttgcccat ataccagcag ctgtagtaag accacttact gattgatttc taataaatat
     3061 taatccagca cctaaaaaac ctataccact gacaacttgt gctgcaacgc gggctgggtc
     3121 tagaccaata cctggagtac ccactatatc attaaatcca tacttggaaa caatgattat
     3181 aagtgctgaa gctaatgcca ctataaaatg agtgcgtatt ccagcttctt ttagtctatt
     3241 ctttctctca tatccaatta aagccccaca gaaaccagca gtaatgattc ttgctatata
     3301 ttcaaattga atcaatatat gatggttcat ttatctagca cccccttcta tataatatta
     3361 tatatactat aatactacat tttttatgtg aatatagtaa agtttaaaat ttatataata
     3421 gttatatatg aatataaaat gtataaacgt atattatgta tataaaaatg atagaaagca
     3481 tctaatttta aatgattaaa gagcaagctt aaataaatca aacttgctct tttaacatag
     3541 tgtatgtttt tatttgtgat agttagataa aaatttatct gtgtttttta catctgcaaa
     3601 actgttctgt agtgatgcca tgaatacttg atgtacagta tttgcattaa tttctatatt
     3661 attccaagat aaattcttcg ttgtacatgc atcgcttatt agagttatgt cgtatcgaag
     3721 tttctttgct gttcttacac ttgtatcaat acacatatgg ctcatcattc cacagattac
     3781 tagattagta atattttttt tctcaaggat actttgcaaa tctgtttcaa agaaactatc
     3841 tggtgtgtgt ttaattataa tttcttcatt atctagtgga aaaacaattt tatttatttc
     3901 tgaaccatga gtattaggaa gaaaaaaaga agctgtttta tctgttgata tatgctgtat
     3961 atgaataact ggtaaattgt tttttctaaa aaacagtagt atttttttag cattttcagc
     4021 agtttcttct gattgaaata attcacattt tccattttta aaataatcat tttgaatatc
     4081 aatcaatatt aaagctatag acatgattat accttctatt tacttttatt ataatactat
     4141 tgtaaaaaat gtaaattact tactttttgg tatgtatagg attacttatt gtagcaaatt
     4201 cctttattat ctagaatttc ttgttgacca tgttcaagca tatattcgat tcctatttct
     4261 tgcattattt ccactgcttt taacattttt ttaccacgat tagtaagaaa atattcaaca
     4321 tgtaatggat atccagcaga acttatttta tcaataatcc caaaatcacg taactctttt
     4381 aattgttgaa tcaacatttt ttgatttatt ccataaatat tatgttgcaa ttcagaaaat
     4441 gttttattag attgtcttaa ttcaaatata aggatagttt tccattttcc tttgattatg
     4501 tcatgtacaa tttcaagagg acatgtatat tcagctctta ttttcatagc atatcacccc
     4561 cagtaaaatg ataataaatt atatcacttg tcttgtgata tgacaaatat atatgaactg
     4621 tatataaatt ttatgtttta ttaagttcta aatttcttct attgtagttc atatttagta
     4681 cataaataat tagtaagata taagtaaata aatatataac atgactatag aaatttaact
     4741 ttaaaataac ttatgataat ttagaggagg aatatatatg aatatatcaa gaaaagctat
     4801 gaaaataata gaattagcac aaaaaattgc aaataagaga ggtatttcag tagaagaagc
     4861 ttggagtgaa gcagtaacag aatataaaaa taaatacgaa catattgcat agatatttaa
     4921 aaatttagaa tatttggtag aatataatga ttttaaatga tactataaat tataaggtat
     4981 atcattatta acatagaagg aggatatctg cttgattgta aaaatagata atactttaga
     5041 aaaagaattt tggcaatatg tatcacatga agaaagttta aatttattca taattggata
     5101 cgttgagaat tatggattta gctctcaata tcaagatata tggtctcagg tagaagatgg
     5161 aaatataacc tctataatat taaaaaataa gtcaacactt ataatttata gttttaaaaa
     5221 taattttgat ataggagaaa tgaagaatca tataaaagat ttagatgtag aaagcataag
     5281 tggaaaaaaa tgtgtaatag atagactaat aagtaaatac aaagattttt atgaaaaact
     5341 agataataaa ttttgtgtat taaaagaaat aaaagaaata gatttttcca acatgaagga
     5401 gtataaaata gaaaatgctc aggaaaaaga tattgatgaa ataggaaaac ttttaaatag
     5461 gtcagactat aaagtaagta aaaattacat agaagaaagg aaagaacatt taaaagaggg
     5521 aaatgtaaga gcatatttta ttagaaatga tgatactatg attagcactg tgtcaactgg
     5581 tatggaaact agttttttgg caatggtggt ctctgtaagt acagataaaa gatatagggg
     5641 aaaaggactt gcaagttata tggtttacaa tttgagtaaa gaattattgt tggaaggaaa
     5701 agttccatgt cttttttata ataatgatgt agcaggtaaa atatatcata atataggata
     5761 caaagagata aatgaatgga caattctttt taaatagcta taaaaataat aaattttcgt
     5821 gtaattctta acgtgtattg tataatcgct caaatacttg aaaggtgtct agtagatggg
     5881 tttgttagca tttatggtct ccttatctaa gtaaagttcc ctttggaaat cggtgaaata
     5941 ctcttttaca tttcatgagt cctataaaat ctatgacata agaaagaccc agttacattg
     6001 agtaattggg tcttattatc atatatattg tgtgcttaca tgatatagtt catttctata
     6061 aacgtagtat ttcgtaagtg ggaaccaatt ttttaacaag tgattatttg aacaaagcaa
     6121 taaggatatg ctagcagatg gattttgtat aatttaaaaa tcaatattta gactctatca
     6181 tgtattttca tcatcataat ccaatttgaa attgtatcta aaaccagttt gctatttcca
     6241 cagttacagt ggttactagc ttgttctgct tctgtcataa gcctaaatgt aagggatcgg
     6301 acattgttga gtaaatcaat ctctttacca acagttctat aatcaataaa atgatcttgt
     6361 gttgcaccta aaatcaaaat gtcttggttt atattttttg caatagaatc tatttgataa
     6421 tacttgattt tctgaaaata ttcatagggt gtagacgctt catatgcgta cattccatgt
     6481 tttaaacccc ataccaccat actatcgcca cttttcattt tttgctttat tataaaattg
     6541 actagccatt ttgttttgag ttttagcagt agtctaagta gattttgcat ttttattggt
     6601 tgtgaaccaa ctattatgtc taagaaattg gggaatacag accaagctac cacgcgttca
     6661 attcgttttt cgaaggctgc tgctcgtgga gcaagaaaac ctcccaagga agcacctaca
     6721 atagttatat tctctaaatg aaaataatct aaaattgtct taacgggacg ttcccattga
     6781 taagtaaagt gttttccctg caaacgcatc actccacctt gaccaggtcc ctcaaaaaga
     6841 taaatatcaa atccctgtga cgcaagataa agcattggga aaaagaattc ttcaaagtaa
     6901 gaatcatttc caccgtggag caatataaca tcttttacct cgccttgcgc ttttgcatac
     6961 attacaggta gggtgatatt ttcatatggt acatgaaaaa tttgtacagt tccatttgag
     7021 aaatattcag agtagtagtt gtagaataga gtagttgctt ttttgtagac tgatatttta
     7081 tcagggtcac catcatacat aaaaaattca ctcatacgat agtaagcaat tgcattttct
     7141 gtgcgtccat cttgttcagc ttcttgtgcc agatacataa gttctgtttt ccaactttcg
     7201 ctatcgacaa ttttagagga aactttttgt atatcttcca agcgcccccc atcccacata
     7261 ataatcctgt ttaactgaaa attaaaattg gcatttgtat taaggtaata tagaccttta
     7321 ttaaaagaaa ttttatttgt tttcaatgtc atcactccta aaaagataat gcatagcaat
     7381 aaagagtaat ctttaatgct atgcatttat tgtaacaaat attagaagta ctgactttca
     7441 aatagacgct attggcggat aaaatatatt ttacattttc ctataagctc acttgcagta
     7501 atgccaagat attttttgct ggaagctgag aaatgagacg gtgaactaaa ccctgcgtcc
     7561 atagcagctg aggtaatatc tccacttttt aacaataact cataagcctt gcgaagtttt
     7621 gataaagcca aatagctgtt tagtgatact cccatttgtt ctttgaaaag atgagaaaac
     7681 cggcttttgg acaattttac ttcggcacat atctcaaaaa agatttcatt gtgaatgtgt
     7741 gtggacttta gaatatgatt caatgcccat tgtattctac tgtctgtaat agacgaaata
     7801 tgataattta aattcaatag acggaaagct tgttttgaaa aatcaatata gctttgggac
     7861 aatcctttta cctgacaagc tgtataaata ttttgtattc gttcaactat aagattatct
     7921 ataacgaaaa aagagtgatt tagtaattgg tgttgactaa tattttgggc gagtatgctt
     7981 gtatcatcta tcaagaaaac caacatttta ttatctttac ttgacaccgt atgcgataca
     8041 ttggaatcaa taataattcc tttacattgt aatgattcgc ccttatcaaa cgtcacagat
     8101 aagttaccag actgtgatat aataatatgt tttgcaagat gtttatggat agttggttgt
     8161 ctatactctg tatctaaaaa tatatggtca atcattacac aaatggtagt catagctgtc
     8221 aactccttgt tcatagctta gcatattata cagtttttaa caagaaagct tagtgattac
     8281 aagtatttaa aaatcctatt ttaggcaatg aaaaataaaa aactctatcg agaattaaag
     8341 tatttataaa taactatatt tttactatga ttcatgatag agttttttaa aatttatgat
     8401 actatttttt attatttaaa taatcaagtg ttttaagttt ataaccttct ttttcccaat
     8461 gtgtaagaac ctcatctaat atctcagtat ttgttgatga atttggatgc agtaaaacta
     8521 ttgcaccagg atgagttctt gaataaattt tgtttttggc aaattcatga gtaggttgtt
     8581 tcttttcata ccaatctaca taagcaaaac tccaaaatat tgatttatat cctaaatctt
     8641 gtgtatattt taaagattgt tcgctaaatt tacccattgg aggtctaaaa tacttaggca
     8701 tttcttttcc agtaacatct ttgtatgctt tttctacact tgtaatttct tctttgaatt
     8761 tttcaaaatc tgtaatacct gccattgatg gatggctttt tgaatgatta caaactaaat
     8821 gtccttcttt ttccatacgc tttattaact ctggattgct ttttatataa ctttcaacta
     8881 cgaaaaattg agctttagca ttgtgttttt taagcgtatc taataatttt ggagtgtttc
     8941 cactttcata tccagcatca aatgatagat atataacttt ttcattggga tttcctacat
     9001 aataagctcc gtatttttta aaaaaatcag cttcttttat aggtgatggc tgtttaccat
     9061 cctccctagg gttaaaatac caatcatatt catgtgtgtc gagtgttgga ctagaaattt
     9121 gagtttttgt tttatctaat gatttaaaat ttatactagc aattccaaaa agtattagag
     9181 caaatgctcc tatcataatg tattttttta ggctatcttt tttcacaatt tcacctctta
     9241 tttaaaattt aaagtatatt tcttataata tagtttgaat atttatagtt aaaaatattc
     9301 acatatacaa ataataaatg aaagaagttt actgtaagat ataaagttga tattaattat
     9361 cttaaataaa gtgtatataa atgataaaat ataaagagaa atgattgtaa ataagttagg
     9421 ctggtttaac ttgaagggat ataaaaatgg ataatataat aagtgtattg aatgatgttg
     9481 tttttttaga tatagaagtt agtggattag attgtttaaa ttcagaaatt ttagaagttg
     9541 gagctgtcaa ggttaaagac tggaaaatat atacctatga atcattaata aaaaacaaat
     9601 ttgaggtacc tatagaagta ttctcagtat gtaaaaattt agataaaaat gatttagaaa
     9661 tagctaatga aatagaactg gtggaagata gacttgtaaa ttttgtagaa gatagtttca
     9721 ttatatgtca tgatttaagt ctgaaaaaaa agttttttga gtatcatatg ccaaagttaa
     9781 aaaacaaatt tattgattta attgaattag cagttatttt agaaccatat cataaagatt
     9841 atagcttaga atacttaaaa aatactttaa caaattgtaa ttctaaagtg gaaaatagag
     9901 ctttatcaga tgctattgat ataataaata ttgtaaattg ccttttagta aaatttaata
     9961 actatgaaaa aactactttg gagccattga gttttaaaat aaattcatat cttaaaaaat
    10021 ttaatcttcc aacatgggaa tggagcaaat ttttagaaga ggcaaattat gatttatcaa
    10081 ataatataaa tataaaaaaa gaatataata tatttgactc aaaagaagaa aagaagaagg
    10141 aaagagaaac cttaaaaatt ttaaatgaag aagaaaaaaa ttatgaagaa ttattaaaat
    10201 acaaaactat ttgggagaat aaagaaggat ttacatatga atatagacct ggtcaatatg
    10261 agcttactaa aacaataaga gagttgttta gaaatagcga agatgaagaa aaaattgcat
    10321 gtatagaggc accaactggt ataggtaaaa gtgtaggata tttattgcct gcaatattag
    10381 aggctagaat taataaaaaa agattattaa tttctactga cacaaaggaa ttgcagatac
    10441 aattaataaa taaagatatt ccaaatgtgt tagattcttt gggtctaaat ggaaaagtaa
    10501 gttacggata tataaaggga aaaaataact atatttgtat agatagactg gaagcatata
    10561 tagatgatta tgagtctcaa aaccctacta aaggagaaat tttatcttta atatttttga
    10621 agagattagt tgaaggtggc aagtatggag atatagaaga aataaattat ttagtgtttg
    10681 acaacttcaa ggaaataagt acccacttaa gaaatgtaag ttgtgaccca aatatgtgca
    10741 gacctaaaaa atgcaaaaaa gattgtttat ataaaaacag aatagaagaa ttaaaagaag
    10801 aacatataac agtagtaaat cattcactat tagctaaatg gccgtataag gatgaaaaac
    10861 ctttagaaaa tataatagta gatgaagcac ataatttgac tgaaaaagga tatgatttct
    10921 tttcaagcat tataaattca aagtctttaa ggtatttatt acaagaaatt tatccttacg
    10981 agtttattca aaatagtagc tttatatata aaaaatatag tagaaatatg agaaaaataa
    11041 aggcttttga taaattttat aatgtattaa aaattggaag agaagataaa caaaaaattg
    11101 ctagaagtat aaatctcatt attgaagaga tagattcaat attaaagttt ggtaattgta
    11161 atgaatataa taatgtaagt aattataatt taagatggga actgaatttg caaatagatg
    11221 aaattgtggg aaaactaaaa aaggatggta tagatactga aataagttat agagcctata
    11281 gtgaaaagat aaagttgagt tgtgagaaaa taataaaaaa tttagtttca ataataataa
    11341 taatttatag gaatattgat gatgatagta tagataaaga agcagatata tataaatttg
    11401 gaaaggcaaa gacaagagat ttagaggata taaagattat atttgaaatc tttttagagt
    11461 atgatgaaaa ggatgattat gcaaggatag ttgaaattga taagaattat aatgactttg
    11521 aatttcgagt agtaccatta aaaatagcag atttatttga agaaaatatt ttatctcaat
    11581 tagaaaaagg aatattttta tcagcaactt tgagtttatc tgaaagtatg tcttatttta
    11641 aaaatacatt aggaatagac agagttaaga atgtggaaaa gattatagaa ccaatttttg
    11701 attataaaaa tagagtctct gtagttggat tttctgatat ttgtgagtac agaaattctg
    11761 aatttccaaa tgagatgagt aaaattatat ctaatatatc aaagattaca gaaggtcata
    11821 cattagctct ttttaatagt aaagatagac aagaaaaaac ttatgaaata ttgaaaaaat
    11881 acttacatag ttttaactta gaaatttatg ctgataaaaa ggggattaga caccttaacg
    11941 atttaaatag aaaatgtgta gtacttggct caaaaggatg ttttgaaggc gtagacattc
    12001 caggggatgg tttggtttgt gtaactttag acaagcttcc gaatctaaat ccaaaagacc
    12061 ctttgtattt tactataatg aagaagtatg gtatagacta ctatactata aattatcctc
    12121 aaatgaccat aaaggtaaag caagctatgg ggagaatttt aagaagcaaa tatgactatg
    12181 gatgttttgt gatttttgat gtaggaacta atataagtgt tttaaagaga ttagagaaag
    12241 atttacacga ttgtaaaatt tcaaaggtta atagcaatga gttttatact tatataaggc
    12301 gacatttaaa taaatctaga agtttaatac taaaatctgt tatatttgat actataaaag
    12361 cgttgaatgt tgatgctaaa atggataaca atgatgttga taaagatatt ataaaaaaag
    12421 atataaatga aaatataaga caaagagctg taaaaggaga agtgtaccat attgatatta
    12481 taaaaaaaga tatgaaagta aaatattttg atagaaacta tcttataaat ttagatatat
    12541 ttatgagaga agaagataaa aattaagagt gggagtgaga tatttggcaa aacaaaagtt
    12601 ttatgctgtg aaaaaaggta aaaatatagg tgtatataat acatgggatg agtgcaaaaa
    12661 acaagtaaat ggattctcag gagctgaata taagagtttt tctacttttc aagaagctaa
    12721 agaatatatt gatgggtcag aaaaattgag ttttcaagaa gataaggagt ttatagaagc
    12781 atatgtagat ggaagttatg aacattcagt aaaaatgtat ggttcgggtg ttgtgatttt
    12841 aaaaaataat gaagttataa aaacttatag tgaaaaagga aaagagaaaa ctttagtaag
    12901 tatgagaaat gtagctggag agatagaggc ttctaaaatt gcaatgcagt actgtattga
    12961 caataatgtt caaaacttga ttttatactt tgactatgaa gggattgaaa agtggtgtac
    13021 tggagtttgg aaaacaaata aagaaggaac tatagcatat aaaaatttct atgacagtat
    13081 taagaataag cttaatgtaa aatttacaaa ggttaaagct cattctggaa ataagtataa
    13141 tgaggaagct gataaattag ctaaaaaagc tattggtgta taataaaaaa atatttatac
    13201 ataattacat ggtataagag tatatctaaa ttcctacttt ataattaaac aacttgtaat
    13261 taaacaactt gtaattaaac aacttataat taaataaatt ttgattaaaa aagcagattg
    13321 tattatgaaa aaataagtat ctaatttata aacaattaat aatagtatta attagataca
    13381 acttatttaa tataataaaa tctgcttttt atataactat aaaatcttcc tacattctat
    13441 tagaattgcc cataaatacc ttcaagttct tcttttaata cttcagggtc atttatacca
    13501 atagacatgg ttctagccag tatatttgct tggtcataag ttgatgtcca tgagaataca
    13561 ccacctagac aatttctaag aacatattga gtttttaaga atattgataa agcatcatca
    13621 tatgacattg caaaatctcc atccttaact aaatacggaa cttgagcagt attatcaaat
    13681 ctatattcat atccattttt atttatataa tcccttctaa gctcatcata agttcttgtt
    13741 atagtggcac ctaatcttcc ataaaatggt ataccgagaa ggattttttc agaaggcatt
    13801 ccagcattct caagatttct caccattgca tcaacactat atcctggcaa agataagtct
    13861 gaatcaaaaa gatttgcttg atgttttcta ccatttgggc ctgtttcacc tgctgtaaaa
    13921 tcataactca taagattaaa ataatctatt ataggagcta ttttatctat ttcagcactt
    13981 gaatttatat atcctctatc tcctgttcca gctacactaa gccatttatc atctcctata
    14041 acatctctta tggcagttag taagagtgta aagttttctc tatcttgagg tcttgatgtt
    14101 attccagatg cactacttcc aggatattcc cagtctatat ctattccatc taaagcatat
    14161 tcatttatca tctgattgac ctgtcttgca aaattatatc tagatgtagg tgttaatgct
    14221 gcatcagaga atccttcagc tccccaacct ccaatagcta caattacttt taagctaggt
    14281 ttttcacctt taagagcaag taattgattt aaaaatctag gataaggaat aaacaaattt
    14341 ccagactggt ctatctcagc aaaagcaaat ataactgcat ctaatagttg agcatctaca
    14401 ttagttggat ttccaagaac atattctgct acaattggat ttatatctgg acagtttgca
    14461 ttttcaacta ttggattgta tgggtctgtt atttgtggtc taactaattt ttttgaatct
    14521 tttaaagttt tcatagctat ttttttagta tttttttcgc tagtataatc gtagttttta
    14581 taattatgtt tatagaaatc ttcataacta ctatcttctt tgttatagct agaatttatt
    14641 ttgtcgcaag attcatcttt atcatagtta aaattcattt catcacaaga cttatcttta
    14701 aacttatctt gtttggagtt gtgaactttg ttagtatttt tagtatgttt tttctcatgg
    14761 ttgcaatcat ctttaatatc agtcttgtgt tcttgtttac agtcaagagc tttgttcagg
    14821 tagtcatgat gttcatgttc acagttacat tcattttcaa tgtttttagt atgttctttt
    14881 ttccagctac aagtattatc aattttcttg ctcacttctt catcattata attatctggt
    14941 acaaaacata agtaccagtc catacatgaa tatttcttat tacaactgtt aactctattt
    15001 tttaattcct tatagttttt aggataaggt aaaatcactg gcattccagg aaaccagttt
    15061 gcaggagtaa ctatattatc tctatcacta gtctgaagtg catctactat tctaagtatt
    15121 tctggaatat ttcttcctgt agttagtgga taataaagaa tcgttcttag aatttgatta
    15181 ttatctataa taaatacaga gcgaacagtt gatgtatcac tcattggttt tgatatcatg
    15241 ccgtataact tggcaattct catatctcta tcttctataa taggaaatgg aatttctaca
    15301 cctgtaagta aagaaatatt atacatccaa gctaaatgtg aactgttact atcaacactt
    15361 agaccaatta gttctgtatt tcttttttta aattcgtcat aatatttagc aaaacataaa
    15421 aattctgtag tacaaactgg tgtaaaatca ccaggatgtg aaaataaaac aatccaatta
    15481 cccttatagt cagagagtct aataggacca tttgttgtat tggctttaaa atcaggagcc
    15541 tttgacccta aacttggcaa atttggcatg taaatcaccc ctcaataaat tatcatttat
    15601 tttattctat taattaatac atattagtgt tactatttta ttaaaagtta agtaatcctt
    15661 atgtcaaaaa aagatattta ttaaaaagat ttaatatttt cctaatataa atgacaatag
    15721 taattgctat ttttttacta atttggtata gtaatggata aggggtagtg tcaattttag
    15781 tgattatacg aggtgattgg attgaaaaaa attctaagaa ttttagttat ctccatgatg
    15841 atatgttttg caatgacttt tgcattagtg ttaataaatt cagttatggg agtacatata
    15901 ggattggatt attacttttt gatgggaagt gtgcttacag tttgtttaac aattggaatt
    15961 gttttatgca atgctagaaa aatatttgat agcaaagcta tacacaagac aagaacgaaa
    16021 actgtcagta ctaataaaaa acaaaagagt cattctaggg atataaaaag aaagatatca
    16081 taatttatgt aaaggaaata tttatgtata aaataatgat tgataggtaa aataaaaaca
    16141 cgatttaact tgtgttttta ttttttttca agaattatga tataataacc aataagaaat
    16201 ttagtttacc ataaaatata aagattatta aatggagagg gaactataat gaattttaga
    16261 gatttatttg agaaggctgt agattttatt gatgaaaaaa ggaaatctat agttgcgatt
    16321 tcattatcaa cattaggtgt aattgctttg ataatagtat ttttcttaag tagtaacgag
    16381 tttagtgtag gaaatgaagc taatgagttg ttaaagatta tagagaaaag acagtattct
    16441 atagctgttg attactacac aagtatagaa aaaaagtttt ctgattcaaa aatggaaagg
    16501 tttaataagt ctgtttctaa aaaaataaat aaattgctat taaatagtgg tgataaatat
    16561 ttagatggag acataagtca agaaagtttt attggattaa taaatactgt gaaagaatta
    16621 aacaaaataa gtatagatgt ggatgactta ttagctcaag ctgatagagt tagagaaatg
    16681 tataaagaag aaaatactac ttatgatatt gccattaact acataagtac tgtttcaata
    16741 ttaaatggta tgggtagtaa cctagatgta tacaaacaga atatagaaac aatcaaagag
    16801 tctagagatg tttatgactc agcagtaaaa gaccaaaaag catataagta ttatgaagct
    16861 attgaaaagt ataataaagt attaaaagaa gatgaaaagt attacagtat ggctcaaaat
    16921 gggaaagaac aatgtattga agaaatgtat gattactata taagtagagc tgaagaggca
    16981 aatacttctg gagattatga aagagcatta caatatattg agtatttaaa agaagattat
    17041 tcagatgatg aaaaggtaca gagtttagaa aaaaaatata aaaagaattt atcattgtat
    17101 accttaacat ctgatgacat tataaatgta atagctaaaa aaagtggcaa agacaaggca
    17161 aatctatcta taaattcact tccacaaatg ataaaaaata ataaatacta ttatgctgaa
    17221 gtatatgagt atgataaatt aataaatgaa gttttggtaa gtgcaaaaac taaagattta
    17281 tattcgtata aagatggtaa aaaagattac aaagtagatt atggtgatgg atactttaga
    17341 atattagaag atggaagtta tcaatttggt attacaaaag ataaagctaa gtttgtgtta
    17401 actaatactc ttgatgaaaa agaaaataag tataaaaaga ttgagatatt agatatagag
    17461 aaagcagata gatatgtaaa aagtaaaaag agtttagaag aattgtttgg aaaatataaa
    17521 aatatttatt attatgctgt agtaaataaa ggcttattta gaggcaaaga agtttatgct
    17581 attaatattt acaatgaaaa aatatatgcc atatcagaaa atggactaaa tgaatattaa
    17641 ttaataatat taaattaata tataattaaa aagtttttgg ggttttaagg cgcattatgt
    17701 agtaatataa taatagggat atataaataa ttttattgta aggagaagta tgggatgagt
    17761 aatgtgaaca aaaagctagt tatagctatt ggagctgtag cattagtagc aataattttt
    17821 ataggcataa tgtgcatgca atttaaaggg gagaaaatag caaaaaacac atatgttaat
    17881 ggagtagata ttggaaaatt aactaaatcg caagcaaaac aggaattagc taaaaaatac
    17941 aaattagaga atgttgagtt taattacaat gataaaagtt ggaaagtaaa gagcaaagat
    18001 ttaaatttat ctactgatct ggataaaaca gtagaaaacg catataattt aaatagaaag
    18061 agtagtttct ttggcaatct atcaaaaact atttcaacaa attttggcaa aaaaagcaac
    18121 ttagtagttg tgataaatta tgataaaaat aaattaaaag cagaaatgga aaaaatagct
    18181 aaagaaatag atgtagatgt aaaagatgct acacttgata taagtggtga aaaagtcaag
    18241 gttataccag attctgatgg tcttaaaatg gatatttcta agagtatgga gaattttgat
    18301 aatcaaacta aaaaaggtaa ctataaaaat gagttagtag taaaagctac tccagcaaaa
    18361 gtaaaaaaag agcaacttac taatatagat acaaatttag gtacgtattc aacaatattt
    18421 aaaacttctc aaataaatag aagtataaac ataaaattag caacagataa tataagtaat
    18481 gtattactta tgccaggaga aactttctca tttaataaac atacagggaa aagaagtaag
    18541 gaaaatggat ataaaagtgc acctgtaata atggaaggtg agatggaaga agattatggt
    18601 ggaggagttt gtcaagtttc atctacgtta tacaattctg tgttatacgc aggtttagaa
    18661 attgttaatg taaaaaatca tactatacca tcaagttatg ttccaaaagg aagagatgca
    18721 actgttgcag atagtggaat agatttctta tttaaaaata atctaaagca tccagtttac
    18781 ataaaaaatt acgtttcagg aaatcaaatt gtttgcaata tatatggaag tgcagaagat
    18841 aaacaaaata taacgatatc aacaaaatta gatggcgttt cacaaactac aatgaaaaga
    18901 gttaatgacc ctacaatgcc taaaggtaaa gaaaaagtag acaaaagtgg aagaaatgca
    18961 tattctgtat caacatatag aacatttaat gatgcaaatg gtaaaaaaat taaaactgaa
    19021 aaaatagcta attcatatta tcctaagaag gaaggtatta tattagttgg gactatggaa
    19081 ccaaaaccag aggaaaaacc aaatacagac gaaaataaga ataatcaaaa tacaaacaat
    19141 caaaatccaa ataatcaaca aaaaccagaa acaccaccaa ctgataataa gcctaatgag
    19201 acacaaccac aaccgcaagc ttaaagttct caagaatagg atttatgtag agtaataaaa
    19261 aatataaatc taaataggag ggattacttt gaatttaaat aatataacga attacgttca
    19321 aagtatttgc gagaatattt ctaatgtatt ggatgtagat gttactttag ttacaaaaga
    19381 tttaactagg atagcaggta caggaatatt cagaggtaaa ataggagaga caatatcaga
    19441 gaagtcagtg tactctcttg ttttaaaaga tggaaaatcc tatgttataa ataaggaaat
    19501 agaagagaac tgtaataaat gtgcaaatag agaaaattgt aaagaattag ctgatatatg
    19561 tactcctgtt aaaatgggag aacatatact tggaatcttg ggtatagctg cgtttaatga
    19621 agagcaaaaa gaaaagatac tttcaaaaga taaggagtta actgcttttg taaggagtat
    19681 gtctgattta atatctttta aactagatga actttataaa caagaagtta agactataga
    19741 tgaccttgag agggaagcta tagaaaatgc cataaagaaa tatggaagta atacagaagg
    19801 tatgaaaaaa gtagctgagg cattaaatat aggtatagct acactctata gaaaagtaaa
    19861 aaaattcaac attaaaagtt aatagtattg aaaaagaggt aaccttttta tgaaaagaag
    19921 aggattacct tttttattat gagaatggtg gaaaaatatg tatagttttg acttgacata
    19981 ttaaatatgt tatcttaaat atatatttta gagtggaggt gagtattatt ttagttaaag
    20041 atataaaaca agacatagaa agcatgccta gaaaaatttt agatatatct ttgatagaag
    20101 ataataaaag tgatttgact ttttttaata tagaagaaga agacggtgat gaaacattaa
    20161 aagcattaaa acagagttat gtgactacaa attataaaga agaaactcta ttaaacttgc
    20221 aaggagcttt aaattcaaag tcagacacaa cgtctaagga gttaaaatta gaatcatata
    20281 aaagatataa caatgcattg gatttagcat ataaaaatta tataactagt gccattaata
    20341 tggttaataa agctctggag ataaatccta aagatattga tatactaaat cttagaggtc
    20401 ttttaaagtt attaaaatgt gattttgcta aatcatttga gagtttttat actgctttat
    20461 gttatgagaa taatcatata tctagaaaat atgtaaatct aatttcatct gaagatttta
    20521 aaactttttt aggaagatac aatcattcta taagatttat aaatgaagaa ttaaattatg
    20581 agtcaataca tattttagag aacataattg aagaagagcc tgagctaata gagccatatg
    20641 taatcttatc tttgttatat gataaactag gtaatgtaaa aaagagagaa ggatatttag
    20701 acaaacttaa agagctagat aaagataatc ctgtatttga aaaaaatgaa gaagaaaaag
    20761 aagatacaag taaaaaggaa gaaataagaa aagtaaaaag aaagaaaagt attttaccat
    20821 atattatagt tggctgtgtt ttaataggta tggggatata tttgattcaa agcaaaaaga
    20881 gaatagaaaa cttgaataac caaataagta gtaaagagga aaagttaagt gaaactgata
    20941 aaaagttagg agaaacgagt aaaaaattag aaaaaacgaa taaggaatta aatgaggtta
    21001 aaaataaaga accagaaaga gagataactg tagctgatga ggaagattta tattatcaag
    21061 ctctaaattt aaagaaaaac aaagaatatg aaaaagccat agataatttt aaaagtgtag
    21121 tttcttctgg taaaactaaa aaatatattt ctgagtcaat atatcaatta gcaataacaa
    21181 ataaattatt aggaaataag gatgaagcta taaaatatta caaaaaatat ataaatactt
    21241 atactaaaaa tgaccagtat tatgatgatt catattacga gttgggtatg ctttattatg
    21301 ataatggcga tttaaaaaat gcacaacaga ctttttatag tttgagaagt gaagttccag
    21361 acagtatgta taacaattca aaaataaaag aaatcttaag tgaaaaatag gtttatatag
    21421 ttaagttttt tgaaacaacc aagtatgtat tttattaaat taaaataaaa ataaatttaa
    21481 aaagagattg tgtcaaatta taactttcta ttttaaagaa agatatttgc ttgttgataa
    21541 aactacaaat atatgatttg aagcaatctc tttaattttt ggaggtggaa aatgaaagtt
    21601 ttcaatcaat tagtaataat tttatctatc tgggtaatag gagagtatat aagttccttt
    21661 atacaaggaa ttatacttat tcctgggagt atagttggta tgctattatt atttctacta
    21721 ctacaattta aagttttaga cttgagtagt atagaaaatg taagtggatt tttcctcgat
    21781 aatatggcta tattttttat tccagcaggt gtatcactta taaagtcatt agacctaata
    21841 agggaaaatg tattagtgct tttattagtc atatgtttaa gtacactaat agtaatgtat
    21901 actactggaa ttattgtgga gaaaatgata aaaaagaagg aagaaggaca aaaaaatgta
    21961 taatgtatta cagacaccag tatttggtat aatagtaaca atagtttttt tcaatttagg
    22021 aagatacatt caacgaaaaa caagtaatcc tatatgtaac cctttattaa ttgcaatcgt
    22081 aggaataata ttgtttttaa gcatatctaa aatcccttat gagaattaca agataggagc
    22141 tgatagtcta aatttctttt taggtcctgt cacagtagta cttgcagttc cactttataa
    22201 acaatttgaa ttatttaaaa agcatatgtt tgagattatt gtaggaatag gttgtggtat
    22261 agtgatttct tttgtatctg tattgataat tggcaaaata gctaattcag atgtaagtat
    22321 aataaattca ctaattccaa aatctataac aacaccaatg ggaatatctt tagcaaattc
    22381 tttaaatggt atagaatcaa taacagttgt agccataatt tttacaggta tttttggtgg
    22441 tatggttgct tctacagtat tcaaactggg gaatataaat catccagttg ctaaaggaat
    22501 tgctcttgga acatctgcac atgctttagg aactacaaaa gcttttgaac ttggagaaat
    22561 agaaggagct atgagtggtg tttctattgg agtttctggt acaattacag taatactaat
    22621 tccaattata atgaatttta tataaaaaat aaaattaaga cgagtacctt gaatacttaa
    22681 aacaaaataa aataaaaaaa atttaaaata aaaactgaac gaaaaaaatg caaataatga
    22741 aataaaaaaa tcatgtactg aaattataaa aagaaaaatt tcatttagga attaattata
    22801 aagagataat tactaaactt gaatttgcta aaataaaata aattactgcg aaaatagata
    22861 tttagaaaaa aatactataa atttactata ttgcaagtta aataataagc agtatcatat
    22921 tatgctagaa aaagtttcat ttaaggcata aatgagatga tttgaacgtt tttaaagatt
    22981 agaatataaa agattgcact agacatatat gaaaaagata atataataga tgatgtaata
    23041 gatgacagtt caagaaatta taaaaaaaat ttatttgaaa tattatctat tacatcagga
    23101 ataatatatg taagagtaag cttttaaaat tctaaagatt tagaaagatt gaagggagaa
    23161 agaaatgagt gaagttaaga ataccctaca aaaacatcca gttttaagat attcaacaat
    23221 gctatttggg atgttgatga catctatagg aataaatggg ttcttaagac cagcacatct
    23281 gcttagtgga ggtgctacag gtatagcaac atctataaac tatttgacaa atataaatgt
    23341 aggtttatta acatttttaa tcaatattcc aatatttatc ttagggttca tataccttga
    23401 aaaagagttt tgtatttcaa gtttggtaaa tatgattgta ttttcattat tattaggagc
    23461 aactcaggaa ataagtaata ttatacctat acatgatata ttgcttcaaa gtgtatatgg
    23521 tggtatatta tctggtttag gagtcggagt tgtatttaga actaggtctt ctcaaggtgg
    23581 tacagatata atagctgcaa tactaaaaat taagaagaat atagaaatga aagacactgc
    23641 tttagcaata aatggcttga tagtacttac aggaagtttt ttatttgggt tggatttagc
    23701 attatacact cttataggtc tatttctaaa tgcgtattca atgagtttta ttaaagatgc
    23761 aatgaattac caaaaatctg ttatggtaat gtcaaatgaa gtcgatttaa tagcagaaga
    23821 tataatgaag agtttagtta gaggtgtaac ttttttagat gcagaaggtg catacactca
    23881 tcaaaaaaag aagattatat atacaatagt ttcatcaaat gagataccaa agataaaaga
    23941 tatagctttg aagtatgata aaaaggcctt tatatctgta aatgatgtta cagaagtaaa
    24001 aggaagaggg tttaaagcaa aagatttata gaaaatttta caagctggga gtttataatg
    24061 taaatttgta tattatatct ctcagcttat ttaaatttag tatatgaaaa taaaggtttt
    24121 atcaatgatt tgtgtatcac tttaaaagat atcattttaa aattaaaatt gaaaaaatta
    24181 ctcaatatgt agaaaacata accactatta ggatataata aatttaatag atttagtaaa
    24241 atatctagga ataacgttgg aggaatagca ttatgaattt atctaaagaa tatgttaatt
    24301 tgtatgataa ttatttgaaa tatataaatg aagtaaacaa agagattaga agtataaaga
    24361 acatgaagaa acgtgtttta aaaaagggcg attttgctcc agaaaatgaa gtattagaat
    24421 tggaaggaaa aacaatacgc gatatagatg atgtcgatac aaaaaaacca aaaaataaaa
    24481 tatataaatt ttcaaatggg gatgtgtatg taggaaaatt tttagaagga aagatggaag
    24541 ggcaaggatc atatactttt tttgttgatg aaggaacggc tatggagtac ataggagagt
    24601 ttaaagaaga caaaaaaaac ggaaaaggaa actttgtatt ttcaaatgga aatgaatata
    24661 taggccattt tgaggaagat atgatgaacg gtattggaaa tatgttatat aatagcaaag
    24721 atgaatatat tggaacttgg aaaaatggaa aaaaagatgg aaaaggtatt tataagtgga
    24781 gtgatggatg tatatatgca ggtgaattta aaggtggaaa gatggaaggg tatggtattt
    24841 gctataatag taaaggggaa gtactttatg aaggagaatg gaagaataat ttgattcatg
    24901 gtaaaggaac ttatatatgg gaaaaaggta aaaagtatat aggtgaattt atgcatggta
    24961 aaaaacatgg acaaggaact ttttatctaa ataatgaatt ggtttatgaa gggacttgga
    25021 aatttgataa gccatccatc ttcgacagaa cattagatga aatattttca ttaagactat
    25081 agctttatct attaatagat aaagcttttt tctaatactt actgtaaata agtatgaatg
    25141 atattgatta gtatacacta tcaatattaa ctcaataaat gtgatattaa aaatttttga
    25201 ttgattttaa gaaggaggga tatattgaag agattaattg ctttagtgtt agtaatactt
    25261 attgttttat tttatagccc tgaattgata gaagaaggat atgatttaaa agaaaaattt
    25321 tttcaaagtg aaatatggaa tgaattttta gacaaaataa atataaataa taattctaag
    25381 aaagataaaa aagaacaaaa tcaaaattta caatcaaata atactgaagg ggagaactta
    25441 ggagagtcag atataaagaa ttttgaaaaa ataagcttag gagataattt aagctatgtt
    25501 ttatcaagta ttggtaaacc tggtagaatt gatattagtg aatatggttt tgattggtat
    25561 gtatataatc aatatggaaa agaatttgca atggtcggac ttgaaaatga tgaagtagta
    25621 gcactttata gtaattctat taattcatgt gaaaatcaag atataaaact aaatcaagat
    25681 agacaaacag tgagaactaa gattacacca ttaaaatata agagaaaagg aaataccaga
    25741 tatataataa actcagaaaa tcaatatgat ataataagta aagaaggtaa gtatattaca
    25801 attttctacg atattcatga agaaaataga gtttgctcat atttaataat tgataaaagt
    25861 actgaagatg agtttaaaaa attatatcca gatgatagag aagaacttaa aaaatgtttt
    25921 gaacttgaag ttatagacct tgtaaatagt gttagaaatc aaagaggatt aaactcattg
    25981 agatacagtg aacaggcaac tttgagttca agaaaacata gtgaagatat gagagataat
    26041 aatttttttg accatataaa taagaagaat gaaactccat ttgacagaat gaaaagagaa
    26101 ggtatagtat atacttcagc tggagaaaac atagcagcag gacagattaa tgcaatatat
    26161 gcgcatgaag cttggatgaa ctctgaagga catagaaaaa atattttggg gaactataat
    26221 aatattggag ttggagtaat atttggagga agctataaaa catattacac tcaaaatttt
    26281 tataaataac taaaatattt gaaaggaagt taaaaaatga catcatggaa aaaaaagaca
    26341 gtatataagt gcttaatagc agtagtcttg ttttgtggaa tagtgttaat atctaatttt
    26401 agtaaagttt ctgctttgat gatggatact aatggaaatg ttttgattaa acatggttca
    26461 agggaaaaaa agctaatagc aattaccttt gatgatggtc ctcatcctaa agaaactagt
    26521 caagttttag atgtattaaa gaaatacaat gtaaaagcta ctttttttat agctggaaaa
    26581 catgcaaaat ggtataaaga accattagta agagcaagta aagaaggtca cgagatagga
    26641 aatcatactt tcaatcatcc tgatataagt aatttaagtt ctagtcagat agaagaggag
    26701 attgttaagt gtgaagacat tttgaaagaa gtaactggaa aaaaacctac attatttaga
    26761 ccaccatttg gaagttatag agaaaaagac ttaatagaaa ttgcaaaaaa gcatgactat
    26821 aaagttgttt tatggacagg tgtagatgtg aaagattgga aaaatccagg agctaatagc
    26881 attgcagata aaataattaa taaagtaaaa aatggagata taatactttt gcatgattat
    26941 gcaactaatg atacagttga agctttagat atgtttattc caaaaatgat tgaaaaaggt
    27001 tttaaatttg taacagtttc tgagttgata aaataatttt taggggggag atttacagta
    27061 tgattcttat gatagacaat tatgattctt ttgtatacaa cctagtccaa tatatagaag
    27121 aattagggga agcagttgta gtaaaaagaa ataatgaaat caaaataagt gacattgaag
    27181 aactaaatcc agaggtaatt gtgttatctc caggaccgtg ttctccaaaa gaagctggta
    27241 tttgcataga tattgttgag cattttaagg ggaaaaaacc tatactagga atttgtttag
    27301 gtcaccaaac tattggatat gtttttggag gagatattat aaaagcacaa cagcctgtgc
    27361 atggaaaagt atatagtatc aaccatacta ataaaggagt ttttagggga cttaaaaatc
    27421 ctttaaatgt cactaggtat cattcactaa taattgattc caatacagtt ccaaaagaat
    27481 tagaaattac agcaattact gataaagggg aaataatggg gattaggcat aagaaatatt
    27541 taatagaagg agtacagttt catccagaag ctattttgtc agagtatggt catgaaatgc
    27601 ttgaaaattt tataacagaa gctagagaaa gggtacatat gtaatatgat aagagagata
    27661 aatactaaat taaattcttt tgagatattt actattttta gaaatgaaca tgacagtttt
    27721 atattagaca gtgctatgga taaagaaaaa ttgggaagat attcttttat aagtagccaa
    27781 ccatttaagg tattaaagta taaggatact gatgaaaatc ctttagaagt tttaaaagaa
    27841 gaactacata agtatagggt tgtaaatgat actaatcttc catttgtagg aggtgctgtg
    27901 ggatatttat cttatgattt aggtaattac atagaaaatt tacctagaac tgctgtagat
    27961 gatattgaga tgcctgatat gtattttgga ttttataatc atgtaatagt tatagaccat
    28021 cttgtgcaaa aaacttatat agctactcca aacatagata tcgaattaga agaaaagata
    28081 atagatgata tagaacaaag aatattaaaa gaagagaaaa aaggtataga tagcatatgt
    28141 tatgaagaaa aagaagtaac acctataaga cttaaatcta attttacaaa ggaagaattt
    28201 aaaaatgcag ttcaaagtgt tagagagtac ataagacaag gagatatata tcaagctaat
    28261 ttaacacaaa ggttcagtgg agaaacagaa cttacaagct ttgaattgta tagggactta
    28321 agaagattta gcccagcacc atttggggca ttcttaaact ttgaagatac acatatatta
    28381 tcaaattctc cagaaagatt tataagatgt gttaataaga gaatagaaac tagaccaata
    28441 aaaggtactc gtccaagagg gaaagataaa gaagaagatt taagacttca acaagaactt
    28501 agaaatagtg aaaaagatag agctgaacta cttatgatag tagatttaga aagaaatgat
    28561 attggcagaa tatctaaaac tggaagtgtt aaagttccag agctatttgt aattgaacca
    28621 tatgcaaatg taaatcactt agtttccaca gttgtaggtg agttaaaaga tgataaagat
    28681 gctactgatg taataaaagc aacttttcca ggggggtcta taacaggagc acctaagatt
    28741 agagcaatgg aaattataga cgaattagag ccaacgcaaa gaaatgtata tactggttca
    28801 ataggttata taggttttaa tggtgatatg gattttaata tagcaattag aacaataatc
    28861 aaaaatgaca aaaaagtcta ttttcaagtt ggaggaggaa tgacttggga ttctgaccca
    28921 gatgaagaat atcaagaaac acttgataaa gctaaatcca taatgaaggc tttgagggga
    28981 tattatgaag aataatgtag gttttgatag tagtttaagt aaatttggaa ttggtttgtt
    29041 tgaaactatt agagttaaaa atggaatagc agtagattta gatatccata tagaaagaat
    29101 gctaagttca ataaattgtt tggatttaga tataaactat aaaaaagatt tcttgataaa
    29161 tgaaattgtt acatatataa aaaaagaaaa tgtcattaat aaagcactta gaattactgt
    29221 ttttgatgaa ggttataata tatctattag ggatattcca tataatcaag agacgtatga
    29281 gaaggggttt aaattagcta tatcgccaat agttaggggg aatagtttaa ttcatagaca
    29341 taagactact aattattttg aaaacatata taccaaaaat atcgcaaata agactggtta
    29401 taatgatggt atatttttaa attcagaagg tgtaatatta gaatgttcta tgagcaatat
    29461 tttctttata aaaggagaaa gagtgtacac tcctagtgat agattaccga tattaaatgg
    29521 aataataaaa aaaaggatta tagagatttg tgacgaatta catatagagt tgatagaaaa
    29581 tgaaattaat atatcagaaa ttagtagttt tgattttgtt tttgtcacaa atagtcttat
    29641 gggcgctata aaggttacag aaattgacaa aataaaattt aataaagaaa atgttgtttt
    29701 tgataaaata gtgaagttat tagaataaat taaaatatta acaatttgtt ggagacaaaa
    29761 gatgataggt tgtatttatg aagtatacaa tgaagagatg actaaaataa tagaaaacca
    29821 aaacactttg gctgtatatt tagtaggttc tagtaaagac gtagatttta ctctttatga
    29881 tgtagagata aacgatattg atgtgttcgt ttttgtaaaa gaaggggaaa aacaagagag
    29941 aatactgttt aaaaaaaagg gaatagaatt tgatgtaaat tacttttcaa aagatggtgt
    30001 aaaaaaatta ttatcaaata gggaatattt ctttgtgaaa gaaatgaaag atgctaaagt
    30061 actgtttgat agggagaata tatcaaatat tataaaagat ataagtagag atatttactt
    30121 agaaggaccc agtaaaatgt cattagagga aaagtctttt ataaagcaag atattggagc
    30181 aaaaatatcg aatttaaaaa ataaagaaaa atttgatgtt tttgaatatc attttttgac
    30241 gaatttatat ttaaaagata taataattgg gtactttaat ataaatgata aatgggtacc
    30301 aaaagacaaa aaattgctaa aagttttaaa aaaagaaaat aatgagcttt ttgaactagc
    30361 gtcaaaagta agtgaaaatt atgactatca aagactttta gatgtttata attatatttt
    30421 taaagaaatt gagacaaaag aggttataaa actaatcttc tagtcacaaa tcaatggggg
    30481 gagtttaaat gaataaagtt gataaagaaa agatacaaca tgcagtaaga gaaatactag
    30541 aggcaattgg agaagaccca gatagagaag gtctaatcga gactccaaat agagttgcta
    30601 ggatgtatga agagatattc tctggtctta gtgaagaacc aagagaccat ttaaaagttc
    30661 tttttgcaga tgaaaaacat gaagaattag tattggtaaa agatattcca ttttattcat
    30721 gttgtgagca tcatctagtt cctttctttg gaaaagctca tattgcttat ttaccaaaag
    30781 gaggaagact tacagggctt tctaaacttg ctagagtaat agatacattg gcaaaaagac
    30841 cacaattaca agaaagaatc actaaaaatg ctgcagatat aataatggaa gaattacaac
    30901 cttatggtgt cttagtagta gtagaagctg aacatatgtg tatgacaatg agaggtgtaa
    30961 aaaaaccagg ttcaaaaact gttacatcag cagtgagagg aatatttgaa aaggacattg
    31021 cgtctagagc cgaggctatg agtttgatta ctatgaagta gcaatttatt atagatataa
    31081 tcatttattg agatataaaa tttaatttaa gggagtgtca ttaattgtgt ttgattatgg
    31141 taaaagaact tatataatgg gaatattaaa tgtaactcca gatagttttt ctgatggagg
    31201 agattttaat aacttggaca tagcaataca acatgcaaaa gatatggttg accaaggtgc
    31261 agatataata gacttaggag gagagtctac acgcccaggt catagctatg tagactcaga
    31321 cgaagagcta agaagagtaa tccctgtaat aaaaaaactt aaacaggaat tggagatacc
    31381 aatatcaata gacacttata aggcagatgt tgcagaagaa gcattaaaat taggtgtcac
    31441 tatggtaaat gatgtttggg gtcttagaaa agataaaaat atggcttctg taataggaaa
    31501 atatgatgca gaagtgtgta taatgcataa tcaagatggg acaaactatg acaaagatat
    31561 aatggagtca ataaaagact tttttaaggt aagtatagaa atggcaatga gttgtggtgt
    31621 aaaaaaagaa aaaatagtac ttgacccagg cgtaggattt ggcaaggatt ttgaacaaaa
    31681 tattgaagta ttgagaagat taaatgaatt aaaagattta gggtatccta ttttgcttgg
    31741 gacatccaga aaatcagtaa taggaaaagt acttccagtt gagcctaaaa aaagattaga
    31801 aggtacaata gctactactg ttcttggtat aagagatggc gtagatatag ttagagtaca
    31861 tgatgtttat gaaaatttaa tggcagctag aatgactgat gcaatatata gaaagtaggt
    31921 ataatataat ggataaaata ttacttagta atctaggatt ctatggatat catggtgttc
    31981 ttaaagaaga aaattttcta ggtcagaaat tctttgtcga catggaactt tatattgatt
    32041 caagagaagc aggtcttagt gacgatataa ataaatctgt aagctatgcc gaggtttaca
    32101 atgttgttaa agatattact gaaaataaac aatttaatct tttggaggct ttagcggaaa
    32161 atattgctga agaagtcttg aataaattta tacttataaa tggagttatg gtcagagtta
    32221 gaaagcctga ggctcctgta aatggtattt atgactattt tggtgttgag ataaggagaa
    32281 caagagatga ataaggctta tttgggaata ggtactaaca tgggtgatag atttgataat
    32341 ttatcaagag catgtgaact tttgaaaaat agtgattcta tatataaagt aaaggaatca
    32401 agtttatatg aaacaaaacc atggggatat acagaacaag cagattttct aaatatgtgt
    32461 gtagaaatag aaacagaatt tgaaccttat gagttgttag aatattgtca agaaatagag
    32521 agagaactac acagagagag aatagttcat tggggaccaa gaactataga tgtagacata
    32581 ctatttttta atgatgtagc ttccactgat gaaagactaa caatacctca tccgaggatt
    32641 caagatagag ctttcgtttt gataccactt atggatttaa atgaagaact tataataaat
    32701 gaaaaaacag taaaagaaca tttaaacctc ctatctgctg aagaaagaga agaagtaaag
    32761 gagcttgtag gttatgaaag aaaacctatt taataataat tcaaagatag agataaatac
    32821 aaataacaaa aagataactg taaaaaaaga taaacttata atagctggac catgtgcaat
    32881 agagagttat gagcaacttt tggagactgc taaatttgta aaaagtcagg gagcaaatat
    32941 tttaagagga ggagcgtaca agccaagaac atcgccaaat tcatttcaag gattaaaaaa
    33001 agaaggtctt aaaatattaa aagcagttaa agacgaagtt ggtatggctg ttattacaga
    33061 acttatggat gtaagagata tcgacgaact gtatagtata agtgatataa ttcaaattgg
    33121 ttcaagaaat atgcaaaact ttactctttt aagtgaagtg ggaaaacaaa ataaacctgt
    33181 tatgttaaaa agaggaatag catccacaat tactgaatgg ataggtgctt ctgaatatat
    33241 agcaatagaa ggaaatagta acattataat gtgtgagaga ggtataagaa cgtacaatga
    33301 ttatactaga aatacattag atttagctgc ggtacccatt attcaaaagg aaacaggtct
    33361 tcctgttgtg gtagacccaa gtcatgccac tggagttaga tatttggtaa aacctatgtc
    33421 actggcatct tttgcatgtg gagcagatgg aataatggta gaagtacatc ctgacccaga
    33481 aaatgctttg tcagatggtg ctcaatctct ttgttttaat gaatttgaag atttaatgaa
    33541 atcaattaat aattattaaa agaggcaaga gcctcttttt tattcgtaat cattgctaag
    33601 gtaaatttat aagttataat ttgatagtta gattatttaa tataaataaa gatttaatat
    33661 tgatagaatt aatataaaaa ataaaaattt tttgattaat aagaaggata tcaataaaaa
    33721 aagtcgaatt gtgtatattg agtgattgac aaatttagaa tatagtgtag aatagaaatg
    33781 ttaacacaaa aaattacacc agaaggtgat aatatgaatg atataaaaga cattattgaa
    33841 gaaataaaag ctagatgcga tatagcaagt ataatatctg aatatatgaa tataaaacaa
    33901 tctggagcta attacaaagg gttatgtcca tttcatggtg aaaagacacc ttctttttat
    33961 ataaatacat caaaacagat ttataagtgt tttggttgtg gtgaaggtgg agacgtaata
    34021 aattttgtca tgaaaatgga aaatttggat tttatggatg ctgtaaaaat tttagcaaat
    34081 aaatgcggta tagaaattaa cactaacatg aatgaagaaa ctaggattaa aatagaaaaa
    34141 tctaagaaat ttcaagatat ccatacggaa gcagctagat tttatctttc aaacttattg
    34201 ggaagcaaaa atcttgggta tgaatattta agaataagag gactagatga taaaattatt
    34261 aagaaatttg gtttaggatt ttctttggat tcatggaatt cacttatgaa tactctaata
    34321 agtaaaggat acaaaaaaca agacttattg gattgcggtc taattgcaaa aaatagagat
    34381 ggaactaatt gctatgataa atttagaaat agagttatgt ttcctatatt tgactataga
    34441 ggaaacataa taggatttgg gggaagagta ttagatgatt ctttgccaaa atacttaaat
    34501 tctccagaca cattaatttt taataaaaaa cagaacttat acggattaaa ttttgctaga
    34561 aaaaatttag aaagtaaaac tatagtctta gttgaaggat atatggattt aatttcgctt
    34621 tatcagtatg gtataaaaaa tgtagtagca acccttggaa cagccttgac tgaacaacaa
    34681 ggcattttaa taaagagata tgcggacact gctattatat cttatgattc tgatgaagca
    34741 ggaataaaag cgactttaag agctatagat atacttacta aattaggtat caatgtaaaa
    34801 gtgttagact taaaagatgc taaagaccca gatgaatttg taagaaaata cggacttagt
    34861 gattataaaa aagctatgga tgtttctact cactacataa aatataaaat agatcatctt
    34921 aaaaaagaat ttaacattca aaaagatgaa gaacgagtga agttcgcaaa agaagcttct
    34981 aaaattataa aacaattgac aagtcctgtt gaaattgatt tttatacaaa gtatttaagc
    35041 aatcaaatag atattaatgt cgagtctata aagagagaag tttatggcaa aaattacaat
    35101 aagccatata ataacaaaaa tcaaaagaaa atagaagaaa aagtaattga aaaagtcgaa
    35161 gtcagacaag acggaaaaca attagttgaa gaaactctaa taaaaataat gttagaagac
    35221 aaaaaaatta gagaaatagc gttacttaaa gtagaagaaa gtgatttttt attgaaagaa
    35281 agtaaagaaa ttttaaatta catgattaaa aatcaagaat tggacaaaat aactattgac
    35341 aaattgaaaa gtttaaacat atccgaagaa tacttaaaag aattaaattc aatttctttg
    35401 aatagtataa acttagagaa cacaaaagaa atagaaggaa taataacaaa tatcagaaaa
    35461 aactcattag aagagcaaat taatagcttg ttgagagaac aacaagaatt agaaaataat
    35521 aatgatatga aagaggtaga tggtagagtt atggaaattg ctttaaaaat agttgagata
    35581 aataaaattt taaagagctt gtagagaaag gaggtttgag tgtggaaaat aaatcgaata
    35641 aaaaagagtt aaaaaaggtt actgctaaga cattaataga aaaaggaaaa aagcaaggtt
    35701 ctctgacact tgcagagata atggaagctt tttcggagac tgaacttgat aaggatcaag
    35761 tagaaaatct ttatgagact ttaggaaatt tgggaataga aataacagaa acaaaaaatt
    35821 ataaagctga tatagatttt tcggttgctg atgacgattt aagtataggc cacttagatg
    35881 aagatgcaga ggcaatttca catgatgact cttctgcaat agaaatagaa actgtggatt
    35941 tatctttacc aaaagggata agtatagatg accctgtaag aatgtactta aaagaaatag
    36001 gaaaaattcc tctacttaaa ccacatgaag aagtagaatt tgctagaagg atgcacgaag
    36061 gtgatgagat agcaaaacaa aggttagttg aagcaaactt aagactagtt gtaagtatag
    36121 caaaaagata tgtaggaaga ggtatgcttt tcttggattt aatacaagag ggaaatttag
    36181 gtcttataaa agcagtcgaa aaatttgact atacaaaagg atataagttt agtacttatg
    36241 caacatggtg gataagacaa gctataacac gtgctattgc agaccaagct agaactataa
    36301 gaataccagt tcatatggta gagactataa ataagctaat aagagtatca agacaactac
    36361 ttcaagaact tggaagagat ccaaaaccag aagaaattgc aaaagaaatg gaaatgacag
    36421 aagataaagt aagagaaata atgaaaatag ctcaagaccc tgtgtcttta gaaacaccaa
    36481 taggagaaga agaagatagc catttaggtg attttattcc agatgatgat gctccagccc
    36541 cagcagaggc agcagcatat tctttattaa aagaacaaat agaagatgta cttggttcgt
    36601 taaatgatag agaacaaaaa gtattaaagc ttagatttgg tcttgaggat ggtagagcca
    36661 gaactcttga ggaagttgga aaagagtttg atgtaactag agagagaata agacagatag
    36721 aagcaaaagc actaagaaaa ctaagacatc caagtagatc aaaaaaactt agagattatt
    36781 tagactaaaa agatacatca ttgatgtttt tgtgataaat gccatgagat aagtgatatc
    36841 tcatggctat tttgtagtaa agatttaatt ttatttttgt agttatttca gtctaatatc
    36901 tataacttgg attaaatcct ataaataaag ggaattgaaa ggagaattat atttgaaatt
    36961 aacagataga ttattgaaaa tagcatcatt agtgtcagat ggtaaaaaaa tagctgatat
    37021 agggacggac cacggataca taccagtata tttgttaaaa gagggaaaag taccatttgc
    37081 agtgctagca gatgttaata aaggacctct tgacaatgca cataaagaag tcatacagaa
    37141 caatctttta gataaagttg atttacgatt gggttctggt atagagatac ttgaaatagg
    37201 ggaagtagaa gaagtgataa ttgctggtat gggtggaatc ttgataagtg agcttctcga
    37261 ggctaaaaaa gaagtagcac ataatgtaga aaaattaata ttacagccaa tgcaagcaca
    37321 agaagaactt agatattacc ttttaaacaa cggatatgaa attttagaag aagttttagt
    37381 gagagaagat tttagaatat atgagataat tgttgccaaa tatacaggta aaaatactat
    37441 aatagaagat gagatatatt atgaggttgg aataaagctt ttagaaaaca aagattctct
    37501 ttttaatgat tttatagaaa aaaagataaa gacatatagt tctatagtta ataaattaga
    37561 agggaaaaat ggggaagcta tagataagaa gagaaaggaa agcgaagttg caataaaaaa
    37621 acttgaaaat ttaataaaat aaataggagg tatatatgct attaaagagt ctaactagaa
    37681 aaattgaaaa aaagtatcct ctaaacttag cagaggattg ggataatgta ggtcttatag
    37741 ttggagattt tgatatggat gtaaaaaagg tattggtatc tttagaagct aatgaagatg
    37801 ttattgatga agctatatcc aaaaatatag atttaattgt gacacatcat ccttttatat
    37861 ttgggaaaat taataaaatt aatagtggag atttaaaagg cagacttatc caaaaactta
    37921 ttaaaaatga tatatcactt tattctatgc atacgaattt tgatatagca tttgatggtc
    37981 taaatgacta ttttatggaa attatggaat ttggtaattc aaaggtttta gatataacta
    38041 aaagcgaaaa tttgtataaa ttagcagtat acgttccaca taactattca gatgaactta
    38101 gaaaggtttt aagtaattca ggagcaggtc atatagggaa ttatagtgat tgtacgtttt
    38161 caatagaagg tgaaggacaa tttaagccat tagaaggctc taatccgttt cttggaagtg
    38221 taaatgatat agaaactgta aatgaagtta aaattgaaac tgtagtacca cagaagcttt
    38281 taggtggggt aataagttct atgttagatg cacatcctta tgaagaagtt gcatatgatt
    38341 tgtacaagtt agaaaataaa ggtgagatat ttggactagg tagaataagt aaacttgata
    38401 aaagtatgac attagaaagt ctttcaagga aaataaaaga gaaattaaat atgaaacaca
    38461 taagagtagt tggaaacctt agtacagaca taacgaaagt agccgttgta acaggagctg
    38521 gttcagaatt tgtaaaaaaa gctaagagac aaggtgcaga agtactaatt acgggtgatg
    38581 taaagtatca tgaggcacaa gatgcacttg atattggaat gtgtatagtg gattgtggtc
    38641 attttgatac agaagatata ttcaaaaatg ttatgaagag atttttagat gaatttagtg
    38701 aaattgaagt aataaagagt aatgtatatt tgaatccatt tagtactatt tagaagtgtt
    38761 tgattaaaaa atactatata aaaaataaat ttgagtttaa ttagggggat aatgatgaaa
    38821 gtagcagtaa tttttcatag tgtttgtggg agtacatatt tattagctag agaatataaa
    38881 gaagtgcttg aagaaatgaa tatcgaggta gatatattta gagtaagtga cgaagttgca
    38941 aaaacacttc ctcaatatta tttgataaat tacaaagaat acaaagacga atttgaaagt
    39001 ataaatgtaa ttaaatctgg aaaagaaata ttagattatg atgctatatt tatgggttca
    39061 ccaacatatt atggaaatgt atctggacca atgaagatgt ttatggatag tttttctgat
    39121 atttgggtag gtgctccttt gagtggtaaa atatttggtt gttttgctac agcaggttca
    39181 caacatggag gaggagaatt agctttgcaa gctatgaata tatttgctca acatatggga
    39241 atgactcttt tgtcagttcc atgttcagta agaggaggtt atccagcgta tggaatactt
    39301 catatagcag gagataattc tgatataaga ccaaatgatg atgccaagat aggtataaga
    39361 gattacctaa aaagacttaa catataagaa tatcatataa aaatacctaa aagtagctgt
    39421 atatactttt aggtattttt acttaattat atacgtgaag ctttaccaga aatatggtca
    39481 atttctattt taacaacagt tgttaagttc atatccttct ctatgtactt catgccttct
    39541 tcaaagaatc ctttagaata tttttttatt atttctacaa gagcattttt cttttcttca
    39601 ttttctacag tgcaagcttt tccaaaaact atagcactag aataagtagt actaaattta
    39661 tctggaataa cactttcatt agcaactact aaaaaagaaa ctttgttgtt tttcgatata
    39721 ttatctaact tatgtccatt tcttgcacaa tgaaagtaaa tagaatcatt aaagtataca
    39781 taatttacag caactccata aggataacca ttttcagata tagtggaaaa agtaccaaat
    39841 tcaccatttt ttaatacttc aacagtatct tcttttgtca tttctctttt tttcaatctc
    39901 atttctttaa acataatatc atcctttcta attttgatgc ttaaattaaa ttttagcaca
    39961 tttataaaga ttatgctatg ttttttacaa attttaagaa aaaaatatag tttaagataa
    40021 aaggtagtga aagagaagct atttggaggt ttatttatga tattattgtt agagtagtac
    40081 aaacaatact aataaattta atagctggtt tcttgttatt taaggtagta agcatgtcta
    40141 taagaagggg aagttatcga gggtagaagt tgttatttat aacgatatta taaagaaaat
    40201 attattatat aaaataataa atagtaaaga ggtatcaagt tgaatataat acttaatacc
    40261 tcttcaaaat taaattataa aagtgttgtg ggaattgtcc tatacatggt aaggagataa
    40321 taaagaatgt atctttaaag tctcaactaa acagcaaaat ttatccaaca aacatacaat
    40381 aaatgcttct ttgtatctag gtggcttaac agtaagtggc cacatgtgtt ttaaaattat
    40441 gtccgtttct ttttcattta tttgaaaaaa taaagttgca ttttttaatg cttctctagg
    40501 gtgagtaaat ccatgtaatc cttttctatc acctttgtaa tgccaatcat aaagaaaaaa
    40561 gtcgtgtagt aaagctcctc taataacact ttgaatatca acacgtaagt gcaatttttt
    40621 acataataga taactataat atgctactga caaactatgt tcaagacatg aaacattgcc
    40681 atgatgagga aatgttctca taagttgaac tttttcagaa gaaagtatct cagaagcaca
    40741 ttcattaaaa tatttgattt cagcttttga caacattatg aaaatctcca ttcttagtaa
    40801 tatataaata ctatacttca acgataatat gaaatatttc ttatcctaca aaatataata
    40861 cccaataata caagtaagat accttttatg gtattttaaa gtaagagaga ttttattagg
    40921 ctaaacaatg aaaaaattaa aagtaaagct atagcaatat atagtatttt tccaagagga
    40981 ttgccaaaat ttatagtcca tccaatacca aaccttttat taaccattaa tgatgggtca
    41041 tttggattat tatacatgat gcctgctatc caatacttct catcttcttc tggagaataa
    41101 gatgagttga attttaaatt tggggaatta atatatgttg ctattaaata aattataact
    41161 aaaagcgttg taatattcat agcagttgat agttttgttt ttattgataa aagagtagta
    41221 aaaaactgtg ttgtcattat aatcatcaat attaaaaaca aataaccaat tttatttaaa
    41281 tatttaatgt tttcaactct actttcttca attctatttg tatctatctt aatccttgac
    41341 tttatggatc caatggaagt tatatataat aaaaccatta aacaaaagtc agtaccgatt
    41401 aatttaaata cattaagaaa tgatttatct ataaaaacat caggagaccc atttatgttc
    41461 caatgagtgg caattgagtc tggcaattta ttataattaa atgctataaa aatactcatc
    41521 cccaaagtaa atagtatcgg tattaaatag attattttaa atttttttat tattttattt
    41581 ttcttattta taaaatccat atctactatt aattttgaat ctacatctat atgacctaat
    41641 ttagaataaa tttctgattt aagcttctta gcttttttat gtgtatgaat atatagtata
    41701 ctctcatata ataaaaaacc tataatacca ataaaatatg aaagttccaa tttattaaat
    41761 gtaaaaacca gaataaaaac taatataaag tctataataa aacctaaaga taatagtttt
    41821 ttaaattctt tatctaattt tataaagtaa ttataatttt tatgctcttg gtctatagaa
    41881 actccataga tgtgtttttt acctacaaaa tagggcataa aataaaatgt agcatacatc
    41941 aaaattagaa ctggaaatat tgtaaataga cattcaatct tactcataat atttcacctc
    42001 atacttgtca aataaacttg aacttagtga caaaaattct tctttactaa tacctgataa
    42061 ataagatttt gccaccaata attccaagtc aagtttattt ttttgacgag ttgtattatc
    42121 tattttcatt ggtagttgag ctacaatagc accttttctt ctatcaatat ttaagaatcc
    42181 ctcttctttt aactcattgt aggatttgtt cactgtatgt aaatttacac caatattttc
    42241 tgccatactt cttacagatg gaagtgattc atttattttc aattctcctg aagctatagc
    42301 cttagttatt tcttctttaa tttgtacata aatagattta tcactattaa aatctaaatt
    42361 taaaaccata aaaaacctcc attatgcttt ataaatgaaa acgattgtta tatacatagt
    42421 ataacataaa aaataataac aaacacaaaa tataagttta tattttgtaa aaattgtaat
    42481 ttaaaaaata acataatttt tattaaaata atacagatac tatttataga tagaaataaa
    42541 aattagaaag gagaaaaaat atgttaacat atttaatgaa aagacaaatg cataaaaaag
    42601 atgatggaat gagtaaacca atgatgatta caggagctgt aattacaggt gtaggagcat
    42661 atgctgtata caaaatggta aaaagccata aatctaattc tcaaatggtc aatacaaatt
    42721 attatggaaa tagcgattat gatgattata aatatgacga atttgattat gactgtgatt
    42781 gtagagacac aaaacaagat tatgaatatg aagaattaaa aaaaaaagta aaagaattta
    42841 attcaagaag aataaattgt gaaaattgtc cacatgtttc tcaagaagaa atgatgcaat
    42901 atgtaaatag tgttaaagat gtaaaggaag atattgattt acatgaagat gctaaaaaag
    42961 ataatattta caaagaagat gaatacaaaa aatatgaaga agaataagta atattttgta
    43021 taactgaaca aatagtaatg tcaaattatt taatatctag ttttaatatg taagttgtaa
    43081 cttaacttga aagaatcagt atcattcttt agatagttaa gttacaactt acattttgtt
    43141 tttataaatt ttaatagttc tttgtgacta ttttgtaatt ttagaaatat tttagtatcc
    43201 agggtaattg tgtataataa aacttgtata gatagatagt atgaaattaa gcagatagtt
    43261 taaaattaaa atgaaaagaa aggaaatagt tatgttaaag ttgttgatag ttgaagatga
    43321 tagtactata tcttttggaa taaagtatgc tttagaacaa gaaggattta gtatagatat
    43381 atcaaaggat ttatctagtg gaaaagaaat gatttcttct aatgaatata gcatgatact
    43441 gttggatgtt acattgccag atggaactgg ttatgagctt tgtaaatata taagaggatt
    43501 ctctcaggtt cctataatat tcttgactgc ttgtgatgaa gaagtaaata ttgtaatggg
    43561 tctcgatata ggtggagatg attatataac aaaacctttt agaattagag aattaatatc
    43621 cagaataaaa gctgttttaa gaagaaaagg taatacttca gaagaaaata agaaaatact
    43681 taaatttgga gatttaagta tatatacatt agaagctaga gtatataaaa atgataaaga
    43741 aatatttctg acatcagttg aatataaact tcttttaatt cttatacaga ataaaaatac
    43801 tgttttaagt agaactcaaa tattggaaaa actttgggat gtaacttatg attttgtaaa
    43861 cgacaatact ctaactgttt atataaaaag gcttagagaa aaaataggtg atgatttatg
    43921 tgaaccaatc tacatagaaa ctgtaagggg gattggttat aaatggatag gaagtgaaaa
    43981 tagtgtttct atataatcca gaagtgaaga agtttttaag taagtatgtt actttgatgt
    44041 ttattgttat cataatatct attggattta gcataatcaa cgtgtcttta actaaagaga
    44101 tggttgtaag aaataatcaa gctataatag ggactttatc gagtaagtat ccaaacttag
    44161 aaagtcagat agtagacata ataactcaag ggaagtctat gaagaacata gattatggtc
    44221 agaaaatatt aagtaagtat aattatgata aatctattaa gataaactct gagcctatta
    44281 tttctaaatt agttttagag actatcaaaa taaatataat attagtatgt ataatattta
    44341 ttttgatatt tgtgttggtt gttagatatt ttacgtcaat ttacaatgat ttatcagata
    44401 tgactaagta tgtttactat agttcagagg gtaagccatt tgatatgaaa aacaagaatc
    44461 aagaagggca gataggtctt ctaaaaactg aactcttaaa gatgacgaca attcttaatg
    44521 aaaaggttga acttttaaaa agagaaaaga tatttttaaa caatacaatt tccgatatat
    44581 cacatcaatt aaaaactcct atgacttctt taataatgtt gaatgattta ttatataatg
    44641 atgtaccata cgaagtaaaa atcgattttt taaataagat aaaaaatcaa ttaaatagaa
    44701 tggattggtt gattaaaagc atgcttaaat tatcaaaggt agaagcaaaa gtgataaatt
    44761 ttaaaaaaga taaggtaaaa tttagtgaac tcatacatag agcaatgcta tctatgaaaa
    44821 tacctatgga gattaaaaat caaaaactga ctatagaggg aaatgataat gtatcttata
    44881 ttggtgatat tgattggtct gtggaagcac tagtgaatat aatcaaaaat tgtgtagaac
    44941 atactcctga atttggaaat ataattataa cctataaaga aaatccatta ttttctgaac
    45001 taataataaa agatgagggt gaaggtatag ataaaaaaga tataccacat gtatttaaac
    45061 gattctatag aggaagaagt agttcaaaag aagatagtgt aggaataggt cttgcaatgt
    45121 caaaatctat aatagaaagc caaaatggcg atatatatgt aaacagcgaa aaaggtaagg
    45181 gtacagaatt tcatataata tttcataaaa tgtatgatag tgactaatct gtaatattta
    45241 attcacttta tagtaatagc caaaacctaa tatatgaagt atagaatata aatattggag
    45301 gtagttatgg aaattttaag agtagaaaat ctaactaaaa gttatggtaa aaatgaaaca
    45361 aaagttgatg ctataaaaaa tgtaagttta tcagttgaaa aaggaacgtt tatagcaata
    45421 acaggtccaa gtggaagtgg taaaagtacc ttattacatt tattaggagg agttgacaga
    45481 ccaactagtg gtaaggttta tataaatgat gttgatatat ataatttaaa aactaaggat
    45541 ttggcgatat ttagaagaag aaatataggt cttatatatc aattttacaa cttaattcca
    45601 gtcttaaatg tgaaggaaaa catattgctt ccagcagagc ttgacaatag agatattgat
    45661 agagaatatt ttgatgattt aatgaaaaca ttaggtctaa ttgatagaga aaagcacctt
    45721 ccaaaccaat tatcaggagg tcaacaacag agaacctcta taggacgtgc attaattaat
    45781 agaccttcta taatattagc agatgagcca acaggtaatt tagatagtaa aaattcaaag
    45841 gaaatattag aattgttaaa attgtcagta aaaaaataca atcagacact aataatgata
    45901 actcatgaca aaaatatggc acttcaagct gatagaatta taagcataga agatggaatc
    45961 ataaaaagtg atgaggtgat gtagatgaat ttatatacat cactaaccat tagatactta
    46021 aaacaaaata aaagaagaac cattgtaacc ataatcggta taatactttc aacagcttta
    46081 atttgtggta taggaaatat atttgaaagt tttatggatt atcaaatgag agaaactata
    46141 aagaatgatg gaagtttcca tgttacattt tatgatgtaa atagaaaaaa tgtggaatat
    46201 gtaacgaaat cagctgaaat agaaaaacat gcatttacca aacaacttgg atattctaaa
    46261 ttagaaaata gtgaaaatgc tatattaagt ataaagcaat atgatcaaaa tgcaataaac
    46321 ggatataaag tttctataaa agaaggtaga tttccagcta aggaaggaga aattgtttta
    46381 agtgaaagta ttattaatct tatagatgat aagttaaaaa taggagataa gataacttta
    46441 aaagtagggg atatgtttga ctcaagcaag aaaaagattg atagtatgac atttcatgaa
    46501 ggtgattata tagcaaatga aaaagataga acttttaaag ttgttggtat aattaaaaaa
    46561 cctgggtttg aacgttataa tggaattgca acagctatag cctactttaa tcctatgaat
    46621 aattacaatg atacaattaa tgtatcagta gcagtaagaa atcctaaaga tgtgtataag
    46681 ataagtaata aaataagtaa aaatatatat tcaaataaag ataaagaagc agtttctgat
    46741 atgataaaat acaatgaaca tttattgagg ttacaaggtg ctagtaagta ttcaaatata
    46801 aatagctcta taaagtctat aattatagca gtaactattt tagtaatcat atgtacaatt
    46861 gctacagtgt acaattcctt tagtatttct ataaatgaac gaaaaaaaca gtttgggata
    46921 ttaaattcta taggtgctac atctagtcaa attaaaagat tagtttttat agaaggtatt
    46981 ataattagtt taattggtat accaatagga ctaatatctg gaacagtagc tatagaccta
    47041 ttgtttaaaa ttataaataa gtattttact gaatcggttg ttactaagat gagtttgcag
    47101 atagtgtata atccaataat aattattgtt agtataataa tagttttgct tacaatcttt
    47161 atatcaattc tactacctgc aatatcggct tctaatattt ctccccttaa tgtgattaaa
    47221 aatactggag agtataaagt gggcaaaatc aaaagctcaa gacttattaa aatgattttt
    47281 aaaactgaag gagtacttgc atataaaaat attaggagaa atagaaaaaa atttataata
    47341 actctttttt ctttaatggt aagtataata atatttatat cttttagtgg ctttacatta
    47401 cttttactaa gaggtgaaga aattagaaat tctcaaagaa aatatgattt gtatttaact
    47461 gcaaagaaca cagctaggtc tgtagaggat actataagtg aactagaaga tattgatggg
    47521 ataaaaaact ttgaattggc aacaggacaa tatgtgtcta taagagtaag tgaaaataaa
    47581 ataaataaat ccaaggaaga tttaatcagg aaatattatc aaaaatataa gattggagaa
    47641 agttatgaat atgatttcag taataatgaa ttgatttttc cgggagattt tgcagtaaaa
    47701 aacaaaataa ataacttagt acagggctct tttaataaag agagagcaat tgaagaaaat
    47761 ggtgttatat tagttagaaa aagtgcaatt gaagaaaatg ggaagaaagg tgtagtagaa
    47821 cttaccaact ataaagtagg agatacagta aattgtgaat atcttgatga aaatgcctta
    47881 agtaaaaaag taaaggtcaa gattcttgct ataactgatg aagaacgtct aggttttggt
    47941 tatcaaaata tggggttaca atttataact tatgatgaag ttgctaaaaa tttaaattta
    48001 aaattaagta aaagccttat atttattgat tcaggaggaa gcattaagac taagaaggaa
    48061 atagaagctt tggcaaataa aaatgatttt aacttttatg atgaaatttc aagcagtgaa
    48121 agtgaaaaac aagatttaaa ggttattaaa atatttgtat atggttttat agtagtgata
    48181 tctttagtga gtgttacaaa tattctaaat acagtaagta caagtataaa tctaagaaaa
    48241 agagaacttg cagtaataca atctataggg gttactccta aaggatttag gaaaatgata
    48301 tatttagaaa gttttatata tggtatactg tcattgttgt ttggaatacc aattagtatt
    48361 ggtataattc taattatgaa taaacttatt tcaggtgtaa ttgaattttc tccgattata
    48421 ccgtggacag ccatagttat ctgtatagta agtgttttta taataacttt tatagcaggg
    48481 tatataccaa tgagtaaatt aaataaagaa aatataatag ataatataag aagagaaagt
    48541 atatagatat aaaaataatt cttaactata taaaatatta ttaaaatcat ttaagtggta
    48601 taaaatatta ttatatcact taaatgattt tttatttata taagaaatct aataataaat
    48661 tactagaggt atttaactat ttagaaagaa ttggtattat ataaagtaag agaatatata
    48721 ataagaaaga atatttgatt ttatttgaaa acaaaagaaa aagaaggtga ttactatttc
    48781 tcatataact gcgaagaata tgtataaaag tttagaggaa agaataaaca agtttccaca
    48841 aggtgctcca ccatctgata cattatacaa aatactaaat gtactatata cagaacaaga
    48901 ggcgaagtta gttgctcagc ttccaataaa gcctttcaga gtaaaaacag cagctaaaat
    48961 ttggagtgta agtgaaagtg aagcatatag agtattggat aaacttgcta gtaaggctct
    49021 gatactagat atagaagata ataaagggaa gaaatatata atgcctcctc caatggctgg
    49081 tttttttgag tttgccatga tgagaacgag acacgatata gaccaaaaac ttttggcaga
    49141 actttactat caatatatga atgtagaaga agattttatc aaagatttat tttattcaac
    49201 agaaacgaag cttggaagag tatacgttca agaggaagtt ttaactaatg ataatgaggt
    49261 tagtatttta gattatgaaa gggctactca tataattgat gaatcaactc atataggtat
    49321 aagtatgtgt tactgtagac atagaatgca acatgttgga aaagcttgtg atgctcccat
    49381 ggatatatgt atgacgtttg acaatgtagc aaattcttta ataaataata aatttgcaag
    49441 acgtgtggat aaaatagaat gtaaagaact acttcatcaa gcatatgaac ataacttagt
    49501 tcaatgtgga gaaaatgtta ggaaaggggt tacgtttata tgtaattgct gtggatgttg
    49561 ctgtgaagca atggtagcag ctaaaagatt tggaaatctt catccagtac agacaacaag
    49621 ttttatacca aatataaacc atgaaaattg tgtaaagtgt ggtaaatgta taactgcatg
    49681 tccaatagat gcgataagca aggttaagga agatggtaaa gaatatataa aaattgatga
    49741 ggatagatgt ttaggttgtg gagtttgtgt tagaaattgc cataaaaata gcattatgtt
    49801 attaaagaga gatgaaaaaa taataactcc agcaaattca gtacatagag cagttctcat
    49861 ggcaattgaa aaagggcagt tacaaaattt aatttttgat aataatgctc tggctagtca
    49921 tagagctatg ggagctatac tgtcagctat attaaagctt gaaccagcta aaaagatttt
    49981 agccagtaaa caattaaagt cagtatattt agataaattg ttaagtatga atgataaata
    50041 gaaactaact taaaaaaggc aaagttttag acttatctat atcttaataa acttatcatt
    50101 taatatttaa atttaatttt tacacatata aaacttataa cttaaataaa aaaaggctat
    50161 attcaattaa tattacttga atacagcctt ttttagttat gttaagctat ttcttttatt
    50221 tgcttaaatg agttttcatt tccatttcca ccaatttgtg ttacagattt aaatctcata
    50281 gttttaaata gttctttttg attataatct agtgagtttc ctactagcat tacaggagat
    50341 ttagtttttc cagccaaaac tccaacagat aatccgtcta ttaaatcatc ttgtttattc
    50401 attccatttt tagcaacata taaattgtct atcttagaat ccttgtaaaa ttcttttatt
    50461 acttttgcat tagtttcatt tctagtgtta cctgatattc tctgtggatt ttgaagttta
    50521 ctttctatat ttttagaaac tgtatattct ccaccgatta cataagattt atcaatcttt
    50581 ttgtttttaa ataagttatt tatttcagtt atattactat tttcattagt aagtattata
    50641 ggcatatcat tttgagctga tacagctcca atgcttactg catcggctaa tcctttttcg
    50701 ccattaacta ctgaaatctt tgatatattg ctaatattgt tcaattcttt agcaatgttc
    50761 atagaagttt gatatctatc acttcctgaa attctagata cagagatatt gtttgatttt
    50821 atagtatcta aagatttttc atttatagaa gcctctccac caactacata tacattttta
    50881 acttttaatc tctttaattc agccaaagtt ttttcattta tttgagagct tccagtcaat
    50941 aatataggag cgtttttctt gtatgcaaat ggtgttgcgg ccaaagcatc tgcaattgct
    51001 gaatcgttta ttaaaactgc attttcagca ttttcccaac ctgcttgact tatcttaata
    51061 gaagtttcat atctatcagt tcctgtaagt gcacttgcat ttacttcatc ttgatatgta
    51121 acctcaaatc tagcatttgg acttcctaag aaaacaatta cgcctccttt tggcaatcta
    51181 actctattgt tctttgtaac taaagaatag tttacaggaa cacctttatc atcataaaca
    51241 gcaaaagctg aattttgagg aagattaact tctatttttt tatttgcgat atcgtcacca
    51301 atcttatacc attttgcata accattggat tctagttcac aagtaaaaga tttttctgct
    51361 ggcaaattag ttatagaatc ttcactaaca tacgttagtg ttccaaaaga taaatactct
    51421 gtaccatttt ctttatgaag tttaatgtcg gataaatcac gacctattac accaggtatt
    51481 tctataaaag catttgagtt attctcatcc attattttag tgttaactat ataacctgga
    51541 gtttcatcag aaagactaaa acttgctttt acagagccaa acatatagct ttgagaagta
    51601 tatttttcat ccactagata gtaacctttg ccacttcttt ttttccacac ttctttgaca
    51661 ctatttgata tattgttgtc atctactttt tgagctacat agtataaact tgctgtctga
    51721 cctaatccag ggacatcatc ataagagctc atatttaaat atgtgatatt atttttttct
    51781 tttacaaatt ttaaacaact gttacccttt tcagatacaa atctatcttg acctatgtat
    51841 acgtatttat cttcaggtcc attttctata taaggagaag atactgttaa agtaccatta
    51901 tcattaacat ctacttttat catatttgaa gaagcataaa gaccactatt ttcttttaga
    51961 gagctaggca ttttaacttg ttgtggctta ctaaatgttt tatcaggttt tatttcttta
    52021 attttacctt tttcttttaa tgcagatagt aagatttctt gtccaattat ttcgttaagt
    52081 tgactacttc caccagaaga aagaactgca actgccatat tttcatctgg aagaactata
    52141 agattactgt ggaataataa agagtctcca ccttttgtaa gagcttttaa attatactga
    52201 ttaaatggat aagtgttgac acaatcccac cctaaaccat atccaagtat agaatcttct
    52261 ccttcaggcc atagaccatt taaatattct ttgttttcca tagcttttac tgaagcagga
    52321 gaaagaatac catttgaatt tttcataaat gtttgtgcaa atgtacatag attttctgca
    52381 cttgaatata aaccacctgc acctatagca ttaaggttat cttgtggaac tgcatcttcc
    52441 caataaggta catatgcttt tgctagtttt gaactatcaa aactattttc tgtagttttt
    52501 gtattttgta agttaagtgg attatttatg tatttatcaa gaaaattagt aaatgacata
    52561 ccagatactc tttctactaa aatttcagct aaagtaaatc catcattaca ataaacggaa
    52621 aaagctcctg gtttagcttt taatctttgt ttttgtaatt cttttaagaa attatcatga
    52681 ccataagaat cattgtctgc taataaaata gtgtttttaa aactacttcc catcaaccct
    52741 gatgaatgat ttaataacat tcttggagtg atttccttgt atctatcatc agccatttta
    52801 aattcaggta tatactttac aacaggagta tctaaattta attttccatc atcaaccaac
    52861 ttcataacag ctgttgtagt aaacatttta ctgatagatg cgatactata catattatcc
    52921 ttatttaagt ttttgttatc ctgtttgctg taaactccac cattaccaga aatctcaatt
    52981 ttgccattat caatcaaagc atattgagca cttacagcac catactttga tagcaaagtt
    53041 tggattttct ctttagcaag agctttagtt tttgtgtaag attccgcgtt tttactataa
    53101 tcatgtaatt ctatatcaga actttgatat ttttcggaat tatcttttgt attatcagca
    53161 aaagtaatat ttggagcagc agtaaataac atactcaata ctaatgtact agataataat
    53221 ttagagatcc tttgcttaaa catattacat tacctctttc cctttttaat aatttataat
    53281 tagcataaca ttcttaatta ttttaatcaa cagattatta tcaataaaaa aagcttttat
    53341 aagtatagct ttttatataa cagagaaaaa tataaaaata atatttgttg ttgtataaag
    53401 aataaagggt ataggtaata tataaataaa atggaggaat tttagacata tgtataagaa
    53461 tataaataag aagcaactta aagaaatgat gaaaaatgaa aaggatgttt tattattaga
    53521 tgtaagaact aaagatgaat ttaaggaata taaaatagaa aatgctataa atatatcttt
    53581 acaagattta atcaataata tagatgaaat ttacgattat aaagataaga aagtagtagt
    53641 ttattgcaga agtggtcata gaagtgttac tgcatgtaat ttgttagctg aagaaggttt
    53701 tgagcattta tataatttaa attcaggaat aattgattat atgagttaaa taaggtaagt
    53761 tatacattaa atactatgta tagctctgtt tattaagtag gaatatatct aaatatttat
    53821 aagataaatt taggaaagaa cgaatttttt cgcttttttc tatgacagtt tttgtagtaa
    53881 atattagttt ttaagttgga aaaaggtagt agctttttag ctactacctt ttaatatgaa
    53941 cttagaatat tattaaaaaa atatatttaa agacagaatt aattttttaa tgaccagtta
    54001 aattactatc gccaaattta tcattaaatg gttttataac tctatttata ggtcctttta
    54061 gaagtacgtt taaacaaata gacagaatca taaatagtaa tagaactagt atatctttag
    54121 ttagatttgg agcatatata ccaccaactg cttcccttgt agcacctatt gcataggtaa
    54181 atggcattat agggtttatt ctttggaaga atgtaggtgt aacttgtata gggaatgtac
    54241 caccagaaga accaatctga acgactaata gtactattgc aattgctttt cctacatttc
    54301 caaatacaga aactaaagaa tacactataa agttaaatac catactagta aacataagca
    54361 gaactatcaa taaaattgga tgttttgctg ttactcctaa caataatata tctccagttc
    54421 ctattataaa accttggaca agagctatac ttaaaaaagt aagacctctt ccaaagtaaa
    54481 tttcataagg tttatagttt ccatgtacag atgtagacaa tagtgctgaa ataagtaata
    54541 taccaaccca tgtagaaaga acactataaa atggtgtcat tgcagaacca taattttcaa
    54601 ttggatataa tttctctgtt ttcacctcaa caggttcttt taagaaatta gactttgtca
    54661 atatatcact ttcaagcaag ctgactaatt ttttcatatc ttcaccatta ctaattttgc
    54721 ttaaagtatc aatcaaatca tccaacattc cttttgctaa aggaagtctt tctcttatca
    54781 atgatatact ttcctgtgca tttcctgaaa gttttagaga agtagtaagt atttcttcaa
    54841 cctttggaag ttttgcttct gccttatcta aaacagtaat tatatcattt gctactttta
    54901 tactgtttgc aaatatacta tttataggtt ttgatatttt tgagtcaaaa ttatttaaaa
    54961 tatttaaatt aattcttcca ataccatttg atagacttaa aacattattc aaagcactta
    55021 tagatggttg ttgtccagat ataactttgt ttttaatatc atttaatgtt gatatagatg
    55081 aatctatttt attgctagag tcttcaagat tatctattac atcatccaat ctattatttg
    55141 atgtaagttg gtttaattta gttaaaaagt ccaccaaagt atcgtttaaa gattgcaaat
    55201 tagaaagttt ttctgataaa ttatctatca acttaggtac atcttcactg ccactattta
    55261 ctgcatcaat taaatttaat gtaagagaag aagcagaaga tgaaaggtca accattaaat
    55321 ttaagtcaga cttaataaga ggagaaagtt cgtctaaatt atcatttgta tcctctaaga
    55381 atttttttaa gtcacttgat aatagttttg tatcattcag agtcttttta attgtaggta
    55441 aatcagattt aatatcttta actgtatcag ataattttga agtagcatca gaagctaaat
    55501 ccacagtctt atttatatgg tcaaatctct tttgaaggtc aataagggaa ttttcaactt
    55561 ttgtcaattt tggaagactg tgttcaattc ctgcaccaac tccgtttgat ataccaagaa
    55621 cgatttcact aactgttttt actatagtct gattaacctg tagttgtatt gtagaagcac
    55681 ctttatctgt aatctttggt gcaactgcat ttattttttc attaacagta tagataattt
    55741 cgcctttttt aacatcatct gtaattaaag atgtcaaatc ttttgtaaaa ttttttggaa
    55801 tcttaagtga tgcataataa gtaccttttt taacaccttc aagagcagtt ttttcatcta
    55861 caaactgcca acctaaactc ttgttatctt tgagttgttc aattaattgg tctcctacat
    55921 taatcttttg tcctgctatt tcatttcctt tatcttcatt tgttactgct acaagcatat
    55981 tagcagtgct tccataaggg tcccaagatg cctttatatt gaaccaagca tacagagatg
    56041 gtaaaaaagc aagacctaaa actattacaa gtacggcata attgccacgt atatctttta
    56101 ggtcactttt aaaaatttct aatatatttt ttaaaccctt tgaaggtctt aaattcttga
    56161 tattattcca gtttaaaatt tcttttaaac tattagtttt tttcaaatgt ctcacctcct
    56221 aaaatatttt gataaagaaa aaagttagat ttataatcaa ttttaaatta tattactttt
    56281 tacacaaata gtaaagttaa aattt
//
LOCUS       DAELXX010000011        56017 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.011, whole genome shotgun sequence.
ACCESSION   DAELXX010000011 DAELXX010000000
VERSION     DAELXX010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 56017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56017
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.011"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            78..194
                     /gene="rrf"
                     /locus_tag="KPH29_000660"
     rRNA            78..194
                     /gene="rrf"
                     /locus_tag="KPH29_000660"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            202..276
                     /locus_tag="KPH29_000661"
     tRNA            202..276
                     /locus_tag="KPH29_000661"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:234..236,aa:Asn,seq:gtt)
     gene            282..367
                     /locus_tag="KPH29_000662"
     tRNA            282..367
                     /locus_tag="KPH29_000662"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:316..318,aa:Leu,seq:taa)
     gene            383..458
                     /locus_tag="KPH29_000663"
     tRNA            383..458
                     /locus_tag="KPH29_000663"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:416..418,aa:Met,seq:cat)
     gene            466..540
                     /locus_tag="KPH29_000664"
     tRNA            466..540
                     /locus_tag="KPH29_000664"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:499..501,aa:Glu,seq:ttc)
     gene            555..628
                     /locus_tag="KPH29_000665"
     tRNA            555..628
                     /locus_tag="KPH29_000665"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:587..589,aa:Gly,seq:tcc)
     gene            634..709
                     /locus_tag="KPH29_000666"
     tRNA            634..709
                     /locus_tag="KPH29_000666"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:667..669,aa:Val,seq:tac)
     gene            715..791
                     /locus_tag="KPH29_000667"
     tRNA            715..791
                     /locus_tag="KPH29_000667"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:749..751,aa:Asp,seq:gtc)
     gene            802..876
                     /locus_tag="KPH29_000668"
     tRNA            802..876
                     /locus_tag="KPH29_000668"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:834..836,aa:Gly,seq:gcc)
     gene            886..959
                     /locus_tag="KPH29_000669"
     tRNA            886..959
                     /locus_tag="KPH29_000669"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:920..922,aa:Arg,seq:tct)
     gene            1935..2774
                     /locus_tag="KPH29_000670"
     CDS             1935..2774
                     /locus_tag="KPH29_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBG1466300.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
     gene            2771..3952
                     /locus_tag="KPH29_000671"
     CDS             2771..3952
                     /locus_tag="KPH29_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466301.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKEDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNIDNPNVISDV
                     DIKVVVESTEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            4256..5158
                     /gene="ptb"
                     /locus_tag="KPH29_000672"
     CDS             4256..5158
                     /gene="ptb"
                     /locus_tag="KPH29_000672"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HBG1466302.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            5180..6259
                     /gene="buk"
                     /locus_tag="KPH29_000673"
     CDS             5180..6259
                     /gene="buk"
                     /locus_tag="KPH29_000673"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBG1466303.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLV
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            6249..6926
                     /locus_tag="KPH29_000674"
     CDS             6249..6926
                     /locus_tag="KPH29_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG1466304.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            6969..7184
                     /locus_tag="KPH29_000675"
     CDS             6969..7184
                     /locus_tag="KPH29_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG1466305.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            7213..8292
                     /locus_tag="KPH29_000676"
     CDS             7213..8292
                     /locus_tag="KPH29_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBG1466306.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            8292..9044
                     /locus_tag="KPH29_000677"
     CDS             8292..9044
                     /locus_tag="KPH29_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBG1466307.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            9045..9602
                     /locus_tag="KPH29_000678"
     CDS             9045..9602
                     /locus_tag="KPH29_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBG1466308.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            9772..11118
                     /locus_tag="KPH29_000679"
     CDS             9772..11118
                     /locus_tag="KPH29_000679"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBG1466309.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILNGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            11385..13217
                     /gene="glmS"
                     /locus_tag="KPH29_000680"
     CDS             11385..13217
                     /gene="glmS"
                     /locus_tag="KPH29_000680"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBG1466310.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEEEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            13670..14965
                     /locus_tag="KPH29_000681"
     CDS             13670..14965
                     /locus_tag="KPH29_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase"
                     /protein_id="HBG1466311.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKMQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKRAVDFSANWLNVYSDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADDENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWGEESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLTKFALD
                     YGLTKDDTVIVSSITPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFISNPK
                     HSCTNFGADIPVNSQLVVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSNSVSLEEYRNIDNTMRNKSEILEMWDKENIDVNKQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
     gene            15428..16165
                     /locus_tag="KPH29_000682"
     CDS             15428..16165
                     /locus_tag="KPH29_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HBG1466312.1"
                     /translation="MKKVKLAISFFILMIIGMTISYADTSINTRYNRLSKALEISEAN
                     FKFYNVKINSVINHNLSNKEFHNIFIEIINNLKLDKKGINWNQKGSGDKIQLCATIKG
                     SDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVG
                     EYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKIN
                     LNIGIKYNEDDGKTLIYIATPIIKLDY"
     gene            16186..17439
                     /gene="murA"
                     /locus_tag="KPH29_000683"
     CDS             16186..17439
                     /gene="murA"
                     /locus_tag="KPH29_000683"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBG1466313.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            17526..18590
                     /gene="spoIID"
                     /locus_tag="KPH29_000684"
     CDS             17526..18590
                     /gene="spoIID"
                     /locus_tag="KPH29_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HBG1466314.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKPESPVSINK
                     TIKKSDIEDKGNKEEKSTVSYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            18656..19324
                     /locus_tag="KPH29_000685"
     CDS             18656..19324
                     /locus_tag="KPH29_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HBG1466315.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFNDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            19422..19682
                     /gene="spoIIID"
                     /locus_tag="KPH29_000686"
     CDS             19422..19682
                     /gene="spoIIID"
                     /locus_tag="KPH29_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HBG1466316.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            19879..20898
                     /locus_tag="KPH29_000687"
     CDS             19879..20898
                     /locus_tag="KPH29_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBG1466317.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDKRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            20914..21342
                     /gene="fabZ"
                     /locus_tag="KPH29_000688"
     CDS             20914..21342
                     /gene="fabZ"
                     /locus_tag="KPH29_000688"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBG1466318.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNVTVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEIEMTTLRKNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(21453..21962)
                     /gene="yyaC"
                     /locus_tag="KPH29_000689"
     CDS             complement(21453..21962)
                     /gene="yyaC"
                     /locus_tag="KPH29_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HBG1466319.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     regulatory      22151..22254
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            22391..23584
                     /locus_tag="KPH29_000690"
     CDS             22391..23584
                     /locus_tag="KPH29_000690"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBG1466320.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEETKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     gene            23900..24997
                     /locus_tag="KPH29_000691"
     CDS             23900..24997
                     /locus_tag="KPH29_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466321.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMAIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSNIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            25261..27480
                     /locus_tag="KPH29_000692"
     CDS             25261..27480
                     /locus_tag="KPH29_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBG1466322.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIASKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEIIKECVMMLVYNQKIHIEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEESFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            27483..28265
                     /locus_tag="KPH29_000693"
     CDS             27483..28265
                     /locus_tag="KPH29_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBG1466323.1"
                     /translation="MKICNDNFIKNFKNMINKCLDFIYPENISCIICDKSIKKTNTYS
                     ICKSCFKEMNFIQDGCMKCGKPIIRHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDV
                     TKKMVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQK
                     IAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKENVKLINNKNILL
                     IDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            28597..30510
                     /locus_tag="KPH29_000694"
     CDS             28597..30510
                     /locus_tag="KPH29_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG1466324.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNVSTKKGSGIYIEGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMLTIEEDMDIRLTDISFKQLML
                     FLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLIL
                     GMNKQINSNNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTM
                     HQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLE
                     RKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTID
                     FIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIR
                     ELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIK
                     QSVLCFARLKTPIKIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDIL
                     SKCDIEKVKDILI"
     gene            30651..30959
                     /locus_tag="KPH29_000695"
     CDS             30651..30959
                     /locus_tag="KPH29_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG1466325.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            30984..31310
                     /locus_tag="KPH29_000696"
     CDS             30984..31310
                     /locus_tag="KPH29_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG1466326.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            31349..32683
                     /gene="celB"
                     /locus_tag="KPH29_000697"
     CDS             31349..32683
                     /gene="celB"
                     /locus_tag="KPH29_000697"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBG1466327.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLISSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGPDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIVTPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSIHEFIFKFL
                     QMPLLKLGNSLGAMAIAYIFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGS
                     KIPNIITGQFQDMFATFGGAGSTLSLVIAMLLICKSKRIKSMGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFAIVPTINIIIAYFCMSAGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQLVLLILGIFMYIPFIKMLDKQYLREENETVEEEDDDISFDDLVL"
     gene            32694..33212
                     /locus_tag="KPH29_000698"
     CDS             32694..33212
                     /locus_tag="KPH29_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HBG1466328.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGVMMEQFLNE
                     SKVIATLFCGDEFFVNNEKEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAIANK
                     EDITLMKEEFCY"
     gene            33308..34267
                     /locus_tag="KPH29_000699"
     CDS             33308..34267
                     /locus_tag="KPH29_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HBG1466329.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIKRRGKAGDLSLVAMNNKGEWGVATNIVDFTFSVV
                     TKEESPAVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            34315..35367
                     /locus_tag="KPH29_000700"
     CDS             34315..35367
                     /locus_tag="KPH29_000700"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBG1466330.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISKKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKTPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSNTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            35370..36044
                     /locus_tag="KPH29_000701"
     CDS             35370..36044
                     /locus_tag="KPH29_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBG1466331.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDIGVVKEIGANGVVFGVLDKNNNIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVDNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISR"
     gene            36597..37148
                     /gene="raiA"
                     /locus_tag="KPH29_000702"
     CDS             36597..37148
                     /gene="raiA"
                     /locus_tag="KPH29_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBG1466332.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDSEINLADEQEDYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            37280..39955
                     /gene="secA"
                     /locus_tag="KPH29_000703"
     CDS             37280..39955
                     /gene="secA"
                     /locus_tag="KPH29_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBG1466333.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMVREDLPDSVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            39970..41077
                     /gene="prfB"
                     /locus_tag="KPH29_000704"
     CDS             join(39970..40044,40046..41077)
                     /gene="prfB"
                     /locus_tag="KPH29_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBG1466334.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNQQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            41221..43362
                     /locus_tag="KPH29_000705"
     CDS             41221..43362
                     /locus_tag="KPH29_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBG1466335.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLENLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYNEAVKSMAPHRVL
                     AINRGEKESFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DSEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKAIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNIGEIDEKIKEIGLRELSEKLEVGQVTLKDIIAEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            43510..44676
                     /locus_tag="KPH29_000706"
     CDS             43510..44676
                     /locus_tag="KPH29_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG1466336.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKTLGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     regulatory      44736..44853
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            44978..45583
                     /locus_tag="KPH29_000707"
     CDS             44978..45583
                     /locus_tag="KPH29_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG1466337.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPLVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVIISLVTLPLYKKMGNIIKK"
     gene            45912..46952
                     /locus_tag="KPH29_000708"
     CDS             45912..46952
                     /locus_tag="KPH29_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBG1466338.1"
                     /translation="MHLSKKIKKLFLDDKGYYFNSNWEDIHIGIYDTKLGDSLKYITN
                     EYIENMERVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYK
                     ALEYVKEQTEYLCLEAIKKDCNALKYVRNKTEGLIIKAISHSSNIDVVSILKALETQT
                     RRICLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVKNQTEKMCIESVRQNGMA
                     LQYVNKQTDKICIEAVKQDGRSLQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYT
                     EDICIEIVRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKA
                     KEYVKIAIDDCI"
     gene            47091..47543
                     /gene="tsaE"
                     /locus_tag="KPH29_000709"
     CDS             47091..47543
                     /gene="tsaE"
                     /locus_tag="KPH29_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBG1466339.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDEGREMILTPKGEFYKEIVEELIK"
     gene            47543..48259
                     /gene="tsaB"
                     /locus_tag="KPH29_000710"
     CDS             47543..48259
                     /gene="tsaB"
                     /locus_tag="KPH29_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBG1466340.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVEFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            48249..48725
                     /gene="rimI"
                     /locus_tag="KPH29_000711"
     CDS             48249..48725
                     /gene="rimI"
                     /locus_tag="KPH29_000711"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBG1466341.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKCIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            48731..49747
                     /gene="tsaD"
                     /locus_tag="KPH29_000712"
     CDS             48731..49747
                     /gene="tsaD"
                     /locus_tag="KPH29_000712"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBG1466342.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREVLSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKNGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSNKALKAVKDKGYNIITLSGGVAS
                     NSGLRAKITELAKDNGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            50044..52752
                     /gene="hpdB"
                     /locus_tag="KPH29_000713"
     CDS             50044..52752
                     /gene="hpdB"
                     /locus_tag="KPH29_000713"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HBG1466343.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKAAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDHRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATPDGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            52756..53013
                     /gene="hpdC"
                     /locus_tag="KPH29_000714"
     CDS             52756..53013
                     /gene="hpdC"
                     /locus_tag="KPH29_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HBG1466344.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDSACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            53006..53956
                     /gene="hpdA"
                     /locus_tag="KPH29_000715"
     CDS             53006..53956
                     /gene="hpdA"
                     /locus_tag="KPH29_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HBG1466345.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFKDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSAENISDIISFMHKNNLIEINLLPFHRLGESKWIQ
                     LGKEYEYSDKGDIDEEHLEELQDIFLDNGIACYVGHETAF"
     gene            54276..55238
                     /locus_tag="KPH29_000716"
     CDS             54276..55238
                     /locus_tag="KPH29_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG1466346.1"
                     /translation="MNKSWSNLIIKNKDKKVRSGWIIITVMAAFYIFEYYLSTILMEI
                     IRKILIATGDINLKTNYFSEYANWLNDVGLQVVFQILTDSLMIIIPIIVWRFIMKNPI
                     SEMGLHSYRIKKKEGYVGMLLGIFNCTVIFIITITIGGGHVTSWVPKISPLTVSWILV
                     FILVAFAEEILNRGFFMAVLRRCKNIYFIMIVPSVIFGLIHIWNPDVTFLSVINIIII
                     GILFSYMFIKSSNIWMCIGYHFTWNVFQGIIYGMPVSGLQVPGIITTQITHGNLLNGG
                     MFGIEGGILTTFVNLLSFIFVWYYYRDSKYDFVSDKVNVTNIKN"
     gene            complement(55337..>56017)
                     /locus_tag="KPH29_000717"
     CDS             complement(55337..>56017)
                     /locus_tag="KPH29_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG1466347.1"
                     /translation="VLKLLNLINNNEFIIINLILTFISSLSIIIYSSNKKNLYPVKNI
                     KLGSLLIIYFMISVIVPTSFILILQGAQPNYSGIIKFIFTPITSLTTIYIFFSEEYAW
                     RGFLQNIFFDKFGKKLGVIILGMCWSLWHLPLIFTLYTPEAPILGVILRSIHIVGISI
                     FLGYLYIKTKNIWFCAIIHVLNNSAFLITNSKESTITYHDILIVSMVVLIFYVPFLFT
                     KEYKNNLE"
BASE COUNT        22430 a         6059 c        10641 g        16887 t
ORIGIN      
        1 taactctaaa gaaactagta ttgctaggtt ctataaactt attaaaaact taatagaaat
       61 attaagcata aagattatgt ggttacaata gcagagagga tacacctgtt cccattccga
      121 acacagaagt taagctctct agcgctgatg gtacttggtg ggaaactgcc tgggagagta
      181 ggacgtagcc acgttaaatg ttccaaggta gctcaacggt ggagcaaccg gctgttaacc
      241 ggtaggctgt gggttcgagc cccacccttg gagccaaaaa agccgaagtg gcggaactgg
      301 cagacgcaca ggacttaaaa tcctgcggga cttacctctc gtaccggttc gattccggtc
      361 ttcggcactt ataaaacaat atcgcggggt ggagcagttg gcagctcgtc gggctcataa
      421 cccgaaggtc gtaggttcaa gtcctgcctc cgcaaccatt aaaacggctc gttggtcaag
      481 aggttaagac accgcccttt cacggcggta acaggggttc aattccccta cgagtcacca
      541 attcaattaa atatgcgggt gtagctcaat ggtagagttc tggccttcca agccagctgt
      601 gagggttcga tccccttcac ccgctccaaa aatgggacca tagctcagct gggagagcac
      661 ctgccttaca agcagggggt cacaggttcg agccctgttg gtcccaccat tcgcggcctg
      721 gtagttcagt tggttagaat gccagcctgt cacgctggag gtcgagggtt cgagcccctt
      781 ccaggtcgcc aaattaaata agcgggaata gttcagtggt agagcgcaac cttgccaagg
      841 ttgaagtcgc gagttcgaat ctcgtttccc gctccataat aacttgggtg catagctcag
      901 ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct gtgcgctcat
      961 taagaaaagt ttactataaa ttatagtaaa cttttatatt gtttaaaaag acacaggaaa
     1021 catgtatatt acctgtgtct ttttatttat aaactttaaa ctatgtaaaa attacagtat
     1081 attctaatag agttacagta tattcattaa tattgtaaac atacggaggg taatgtataa
     1141 tagtctacaa gatgtttatg aaggcatcag ttaaatataa ctaattagat aagagttata
     1201 tacattatat tgtatacttt atggtattta tatattgaaa taacttatga aattatttat
     1261 aaagagattt atgagcatat ttttgtctaa acaataagta taagaaatta taaaaaatta
     1321 gagactatag tgaattgaca atttaatgta atatttataa gttatactaa gtaaattatt
     1381 atactgtgaa atggatatat aactttaggt attgtgtatt attttcactg taggagatta
     1441 tatctagtta tacataataa gttttaatga tagtttatag taagatatta aaataaattt
     1501 atctaatcta gatatgaaat aagttattac tttaatataa aaatgaatct aatggaataa
     1561 tattgatgat aagtaatatt tagattttgt atttgtttat agttaataag tggattatag
     1621 aatttataat aattaatata tattaaacaa agatatatta agatatttta gaacataaaa
     1681 ctgcattaag gcgcgagtac tattttgtat tcgcgccttg atggtttgag ttatttgagt
     1741 aaaaactaac tttaataaga aatatgtatt tattaagttg atatttttaa tgtgtttaga
     1801 gattatttaa caatctccaa atataaaact agaattattt acataaaagt taaatagttt
     1861 gtatagagtt agatttatat agtataatac ttagtgtaaa taattaagaa gttatcaaac
     1921 aggaggataa aactgtgtta gatataaatt cttttttaaa tggatttttt aatataatat
     1981 taagaatgag tatatatgat ttaattgaca tatctatagt agcgtatatt ttttacaaaa
     2041 tatttatgtt tataaaagat acaagagcag aacaagtgtt taaaggtata atatttcttt
     2101 tattagcaac tcaacttagt aacaccttta aattgcatac cgtttattgg atatctctta
     2161 aagctttaga ttatggagtt atagctgctc ttataatttt tcaaccagaa tttagagcag
     2221 gacttgaaca tataggaaga gcaaagttta atttatttgg aaagaatgta aatacttctg
     2281 aagaaactct caatagaaat atagaagaaa ttgtagaagc tttatattca ctttctagac
     2341 aaaaaatagg agctcttatc ataatggaga gagaaactag aataagtgat ataataaata
     2401 caggaactat tattgatgct gaaatttcta ggcaactatt aataaatata tttataccaa
     2461 atacaccatt acacgatgga gctgttgtaa ttagagattc taaggtaaaa gcagctgcat
     2521 gttttttgcc attgacggaa agtaaggatt taagtaaaga tttgggtact agacatagag
     2581 caggaatagg ggtaagtgag gtgtctgact gtataactct gattgtatca gaggaaacag
     2641 gtggcgtatc aattgctaaa gcaggtaagt tatatagaga tatttctaga gaaagaatga
     2701 tgaatatatt acgtagtaat ttaaaaacaa atacagaaac aagaagtttt ttcaaaggtg
     2761 gtatatttaa atgataaata gactaaaaaa taataccaaa ataaaaataa tatcattttt
     2821 aagtgcaatt gcattatgga tgtatgttat ggctgtagtt gaccctgaag aaactaaagg
     2881 gtttgaagat atacctgtat ctataagtaa tatgagtgat ttaaaggata aaaatcttgt
     2941 tatatatcca gacgaaaaat taacagcaga tgtatatata aaaggtagtt tatcaaaggt
     3001 acaaagtgtt aagaaagaag atatacatgt atatggaacc attgaagacc caatacaagg
     3061 acagaaggaa gtatatttaa aagcaaatat tcctcaaggt gttactctag aatttaagca
     3121 tgatgtttta gtagtaaatc ttgaaaaaaa cataaaagaa aaaagagata taaaggtaga
     3181 agtaagtgga aattcaaaaa gaaatataaa taaaattgaa cttagtaagg aatttgtcga
     3241 tgtatcaggt ccaagaactc tagttaaaga agtcaaaagt gtaagagcta atctagatgt
     3301 tggtaatgag acagatgatt tttctaaaaa attgcaatta ataccattaa atagtgatgg
     3361 aactaaggtt gaaggagtaa cattaagttc atcctttgtg actgcaaaga tagaactatt
     3421 gaaggaaaaa attgtcccaa taagagtaag tctaaatgat aataaagata atgatgtact
     3481 gaaaaactat aaattaagta aggaacaagt tacaataaaa ggaaagaaag aagatataga
     3541 caaaataagt tatattaaga caaaacctat tgacttagct gatttggcta atggtgaaga
     3601 aaaagaagtt agcttagaaa ttccagaggg tataactctt gaggatagct ctataacaat
     3661 taaattaaat gatacaaaac aattgagtgc agaattttta tataatgcgg gagatattga
     3721 attaagaaat atcccaacaa atatagataa tccaaatgta atttctgatg tagatataaa
     3781 agttgttgta gagtcaacgg aggatttgag taagttaaat aaaaaagata tttcattata
     3841 tattgattta tcacaaggcc ctgaggaaga tagtaaatat aaaataaaat atgaaacaac
     3901 atatcaattt aaaaaaataa atatagaacc taatattgtt gaagtggaat gaacaaatat
     3961 ttcgaatatt tagttgttac aagatgcgac aaaaaaacct ataaaattaa gtttgttaat
     4021 tttatgtttt tgttgcatct tgtttatttt agttatatta taaaataagg acaatgagat
     4081 atttgttaaa tctaaaaagc gctagatata tgaaaaaaga ctgataaata gatttaaata
     4141 aatattttga attaaatatt tcaattatct ctaggaaaac gttgctgaac gagttataat
     4201 ttaattgaga tataaaaagt tctatttcaa aaaattcaag atgaggtgga aatgcatgag
     4261 aagttttgaa gaagtaatta agtttgcaaa agaaagagga cctaaaacta tatcagtagc
     4321 atgttgccaa gataaagaag ttttaatggc agttgaaatg gctagaaaag aaaaaatagc
     4381 aaatgccatt ttagtaggag atatagaaaa gactaaagaa attgcaaaaa gcatagacat
     4441 ggatatcgaa aattatgaac tgatagatat aaaagattta gcagaagcat ctctaaaatc
     4501 tgttgaatta gtttcacaag gaaaagccga catggtaatg aaaggcttag tagacacatc
     4561 aataatacta aaagcagttt taaataaaga agtaggtctt agaactggaa atgtattaag
     4621 tcacgtagca gtatttgatg tagagggata tgatagatta tttttcgtaa ctgacgcagc
     4681 tatgaactta gctcctgata caaatactaa aaagcaaatc atagaaaatg cttgcacagt
     4741 agcacattca ttagatataa gtgaaccaaa agttgctgca atatgcgcaa aagaaaaagt
     4801 aaatccaaaa atgaaagata cagttgaagc taaagaacta gaagaaatgt atgaaagagg
     4861 agaaatcaaa ggttgtatgg ttggtggacc ttttgcaatt gataatgcag tatctttaga
     4921 agcagctaaa cataaaggta taaatcatcc tgtagcagga agagctgata tattattagc
     4981 cccagatatt gaaggtggta acatattata taaagcttta gtattcttct caaaatcaaa
     5041 aaatgcagga gttatagttg gggctaaagc accaataata ttaacttcta gagcagacag
     5101 tgaagaaact aaactaaact caatagcttt aggtgtttta atggcagcaa aggcataatt
     5161 ttaaggggga aataggcaaa tgagcaaaat atttaaaatc ttaacaataa atcctggttc
     5221 gacatcaact aaaatagctg tatttgataa tgaggattta gtatttgaaa aaactttaag
     5281 acattcttca gaagaaatag gaaaatatga gaaggtgtct gaccaatttg aatttcgtaa
     5341 acaagtaata gaagaagctc taaaagaagg tggagtaaaa acatctgaat tagatgctgt
     5401 agtaggtaga ggaggacttc ttaaacctat aaaaggtggt acttattcag taagtgctgc
     5461 tatgattgaa gatttaaaag tgggagtttt aggagaacac gcttcaaatc taggtggaat
     5521 aatagcaaaa caaataggtg aagaagtaaa tgttccttca tacatagtag accctgttgt
     5581 tgtagatgaa ttagaagatg ttgctagaat ttctggtatg cctgaaataa gtagagcaag
     5641 tgtagtacat gctttaaatc aaaaggcaat agcaagaaga tatgctagag aaataaacaa
     5701 gaaatatgaa gatataaatc ttatagttgc acacatgggt ggaggagttt ctgttggagc
     5761 tcataaaaat ggtaaaatag tagatgttgc aaacgcatta gatggagaag gacctttctc
     5821 tccagaaaga agtggtggac taccagtagg tgcattagta aaaatgtgct ttagtggaaa
     5881 atatactcaa gatgaaatta aaaagaaaat aaaaggtaat ggcggattag ttgcatactt
     5941 aaacactaat gatgctagag aagttgaaga aagaattgaa gctggcgatg aaaaagctaa
     6001 attagtatat gaagctatgg catatcaaat ctctaaagaa ataggagcta gtgctgcagt
     6061 tcttaaggga gatgtaaaag caatattatt aactggtgga atcgcatatt caaaaatgtt
     6121 tacagaaatg attgcagata gagttaaatt tatagcagat gtaaaagttt atccaggtga
     6181 agatgaaatg attgcattag ctcaaggtgg acttagagtt ttaactggtg aagaagaggc
     6241 tcaagtttat gataactaat gataaaagaa taagaatcat aactggtcac tatggaagtg
     6301 gtaaaagtga atttgcgatg aactatgttg ttaagttaag agatatggtt tctggcaaag
     6361 ttgctatagc cgatttagat gtggtaaatg tatactttag aactagagaa aagaaagagt
     6421 taatgaaatc cttaggtata caaccaatag atagctcaat taatgctcca acattagatt
     6481 taccagcagt atcagcagag gtaatgtctc ctatggtaga ccattcatac aattctgtta
     6541 tagatttagg tggagataat gtaggtgcta gggttatagg aagatttagt catttgttaa
     6601 aagaaggcga ttatgatatg ctttttgtaa taaatgcaaa tagggagaaa actcaaactt
     6661 ctgaagaagt tatccaatat ataaaagaaa tagaaaaatc ttctaaatta aaggtaacag
     6721 gtctaattaa taatactcat cttattaggt ttactactat tgatgatgtt ttaagaggtc
     6781 agaaagttgc aaaagaagtt tctgaaaaat gtaatatacc gataagatat gttgcatgtt
     6841 tagaaaatct tgttgaacag ttaccaaaag atttagaagg agaaattttt cctataaaat
     6901 tatatatgag agaagactgg atgtagtttg agctcaaata tatagaaagt ttaaggaggc
     6961 ttaggtaaat ggctaaagga aaagtatctt ttaatcaaga acgttgtaaa ggttgtggat
     7021 tgtgtgttga agcatgtcca gtaaaaatca tacaattaga ctcaaatgta ataaacaaaa
     7081 aagggtataa cccagcaact gtttttgaaa tggaaaaatg cataggatgt gcaagttgtg
     7141 caactatgtg tccagatgta gttataacag tagaaagaga ttaatttaat taatatttaa
     7201 gggggaattt taatggctaa gatacttatg aaaggtaacg aagcatttgg aaaagcagct
     7261 atagaagctg gttgcaaata tttcttcggt tacccaataa ctccacaaag tgaattacca
     7321 gaatatttat caagagagtt acctaaaata ggtggagcgt tcgttcaagc tgaatcagaa
     7381 gtttctgcga taaacatggt ttatggtgga gcaggtgcag gagcaagagt tatgacttct
     7441 tcatcttcac caggagttgc tttaaagcaa gaaggtataa catatgcagt aggagcagaa
     7501 gttccttgtg ttgtacttaa tgtaatgaga ggtggtccag gacttggaag tatacaacct
     7561 tcacaagctg actactttat gtctacaaga ggtggaggaa atggagacta cagaactcca
     7621 gtattcgcac cagctacagt tcaagaagct gttgatatga taatggaagc ttttgacgtt
     7681 gcagattatt acagatcacc agttatggta gttgcagatg gtatgatagg tcaaatgatg
     7741 gagccagttg aatttagagc tcctgagaag aaaagagaat tgcctcctaa agattgggca
     7801 actgttggaa ctaaaggaaa aagaaaacct aatgtaataa actctctata cttagaacca
     7861 gaagtactag aagaccattg ttggcattta caagaaaaat ttgatgcaat ggaaaagaat
     7921 gaagtacaat acgaaatgta caaaacagaa gatgctgaat ttgtatttgc agcttatgga
     7981 actacttcaa gagtagtaaa aagcgcaatt gatatattaa gagaagaagg aataaaagca
     8041 ggtcttataa gacctaaagt attatggcca ttcccattcg aagcattcaa ccaaattcct
     8101 aatgctagaa acatattgac tgttgaaatg agtatgggac aaatggtgga agatgttaaa
     8161 atggcagtag aaggtaaatt accagtttac ttccatggaa gaccaggagg aatgactcca
     8221 acaccagcag aaatagttga aaaagctaaa aaaattattg ccggagaatt ggttgcagga
     8281 ggtgctagat aatggctgtt gtatttaaga aaactgaagg attacaagat actcaaactc
     8341 actactgtcc aggatgtaca catggtataa tccatagatt agttggtgaa gtattagaag
     8401 aactaggagt attaggtgat gctgtaggtg ttgttccagt tggatgttca gttttaggat
     8461 ataaatattt taactgtgac actcaagaag ctgctcatgg tagagcgcca gctgctgcta
     8521 caggaataaa aagagttcat cctgaaaata cagtattcac ttatcaaggt gatggagact
     8581 tagcttcaat tggtactgct gaaatagttc atgcagctgc tagaggagaa aaaataacta
     8641 ctatatttgt aaacaataca acttatggta tgactggtgg acaaatggct ccaactacat
     8701 tagttggaca aagagctact actgctcaat caggaagaaa tgctgaaact caaggttatc
     8761 caataagagt aagtgaaatg ttagctacat taacaggagc tgtatttgta gaaagagtag
     8821 cagttgatac tccagctcat gttagacaag ctaaaaaagc tataaagaaa gcattccaag
     8881 ttcaacaagc aggattagga tttggaatag tagaagtatt atctacttgt cctacaaact
     8941 ggggactagc tccaaacgat gctttacaat ggttaagaga caacatgata ccatactatc
     9001 cattaggaaa tttcaaaaat gttgaagttg aggaggtgaa gtaaatgtct acagctagag
     9061 taatatgtgc aggcttcgga ggccaaggtg ttatgtctat gggacaatta cttacttatg
     9121 caggaatgct agaaggaaaa gaagtttctt ggttaccatc ttatggacca gaaatgcgtg
     9181 gaggtacagc aaactgtgca gtaactgtat ctaatgagcc agttggttct ccacttataa
     9241 cagatgatgc aactgcagct atattattaa atataccagc atttgaaaaa ttcaaagatg
     9301 atgttattcc tggtggaaaa ataatagtaa atagttcttt aataaaagaa aaagttgata
     9361 gaacagatgt agatgtttat tatataccag caaatgaatt agcagctgaa ttaggaaatg
     9421 ataaagttgc taatatgata atgttaggcg cttacttaaa agtatctgat actgtagata
     9481 tagaatctgt actagaagca ttcaaaaaag ttttcggacc tagaaaagaa aaattcgttc
     9541 cattaaacag agaagcttta caaaaaggta tggatgctac atgttctaaa gcaactaact
     9601 aataaagaaa aagatgtcaa aaaaaagaca atagaattat ttctattgtc ttttttgctg
     9661 ataagtatta aaatgttttt atgactgaaa ttaagcctat aatgatattt tatgaactat
     9721 aaaaatataa tacaaattac aagcttttca attatgaaag gagaatcttt agtgagaaag
     9781 tattttggaa cagatggagt tagaggagta gcaaatacag aactaacatg tgatttagca
     9841 tataaacttg gtagagctgg tggatttgtc ttagctcaag gagaccacag agtaaaagta
     9901 gtagtaggta aggatactag aatatcaggt gatatgttgg aagcatcttt aatagcagga
     9961 cttatgtctg ttggatgtga tgttattact gttggaataa taccaacacc tgcagtggca
    10021 tatttaacta gaaaatatgg tgctgattgt ggtgtggtta tatctgcatc tcacaatcct
    10081 gttgaatata atggaatcaa attctttaat aagaatggat ataaattaga tgatgaaatt
    10141 gaattgaaaa tagaagaata tatagacgat atagacaaaa ttgattgtct tccaattggt
    10201 gaaaatgtag gaagaaaatt acatgaacat tgtgcacaaa gagactatgt agactaccta
    10261 aaatcaataa taagcactga ctttaaggga ttaaaagtag ttttggactg tgctaatggt
    10321 gcgtcatata aagtggcacc aatagtgttt gatgaactag gagcaagtgt aatttcaata
    10381 aatagttcac cagatggaaa taatataaac tataaatgtg gctctacaca tccagaacaa
    10441 cttcaaaggg ctgttttgga acataatgct gacttaggtc ttgcatatga tggagatgct
    10501 gatagattaa ttgcagtaaa tgaaaagggt caaattgttg atggagacca tataatgata
    10561 ttgagtgcct taaatttaaa gaaaaataat aaattggcgc aagatacttt ggtagttaca
    10621 gttatgagta atatagggtt gacaatagct gctaaggaaa atggaataaa tttatctact
    10681 acagcagttg gagacagata tgttttagaa gatatggtta aaaatggata taatcttggt
    10741 ggagagcaat ctggacatat gatattttta gattacaaca ctactggaga tggagtgctt
    10801 agttctctaa tacttgcaaa tataatcttg caagaaaaga aacctttatc agaaattgca
    10861 tcaataatga gccaataccc acaagtatta gtaaatgcaa caataaaaaa tgaaaataag
    10921 aacaaatata tggaatatcc agaaataaaa actgagatag aaagaataga atctatttta
    10981 aacggaaatg gtagagtttt gataagacca tcaggaactg agccattagt tagagttatg
    11041 ttagagggta aagaggaagg tcaaataaaa gaattagcaa caaacttagc taatcttata
    11101 caagaaaaat tatcgtaatt taaagggtat tctcagatac aaatactgag gacacctttc
    11161 taagcgccag aacttagtta tagacataat tattgagtaa tataggaact aagttgacga
    11221 ggtcagagtt aatcgaattg tcggcggatg ctctgcggtg tgtttaccat cgaaagactt
    11281 ctttaaataa taggagcaat cctatggaca aagggaagcc aaagtaaact tatatgacct
    11341 aactcttaaa atatataaat aatattttta ggaggacaca aataatgtgt ggaatagttg
    11401 gatatttagg aagtagaaag gcagcagaag ttatagtaga agggctttct aagctagagt
    11461 acagaggtta tgactcagca ggagtagcag ttaatagttc taatgaagaa gaacttaata
    11521 ttagaaagtt taaaggtaga ctttctgtac ttgcagaaga cttagaaaag aatcctattg
    11581 atggaaattt aggtataggg catacgagat gggcaactca tggagaacca tctgatgtaa
    11641 actcacatcc tcattttaat caagccaaaa caatagcagt tgttcataat gggataatag
    11701 aaaactatat ggaaataaaa gaagaattaa tttctgaagg tgttaagttt gaatctcaaa
    11761 cagatacaga ggtaatagca catttagtag ataaatacta tgagggtaat ttattagatg
    11821 ctgtatacaa aactatatca aaattaagag gagcttatgc attaggtgtt atatgtaaag
    11881 aacatggaaa tgaattagtt gcagtaagaa aagatagtcc tcttgtagtt ggtgttggtg
    11941 aaggtgaaaa ctttatagca tcagatatac ctgcattatt aaaatatact agagatgttt
    12001 atttcttaga aaatggagaa gtagttcatc taaaagacga aaatgtaaca gtttatgact
    12061 ctaatagaaa tcttgttgaa aaagaagtat ttcatgtaac ttgggatgta gaagctgcgt
    12121 ctaaaggtgg atatgactac tttatgtcaa aagaaattca tgagcaacca acaggagtta
    12181 gagaaacttt agaaagaaga cttgatgata atggaaatat aattctagac agtataaata
    12241 tttctaaaga agacttagaa aaaataaata aagtttatat agtggcttgt ggaacagcat
    12301 ataatgctgg attacttggt aagtatgcca tagaaaaatt tgtaaatata ccagtaataa
    12361 ctgatatagc atcagaattt agatatagcg acccatttgt agatgaaaat tcattagtaa
    12421 tattagttag ccaatctggt gagacagcag atactctagc agtattaaga gattcaaagg
    12481 ctaaaggagc aagaatactt tcaataacta acgttgtagg ttcatctata gcaagagaat
    12541 cagatgatgt attctatact tgggcaggac ctgaagtagc agttgcatca actaaggcat
    12601 atactactca aataacttct ttatacatga tagctttaga ctttgctatc aaaaaaggaa
    12661 caataactag agaattttat gatagtatga tttctaagat gaaagaaata ccatcaaaaa
    12721 tacaagaaat attagacaat gaagaatata taaaagaagt tgctaaaaca gttgttagtt
    12781 ctgaacatgc attctattta ggaagaggta tagactatag cttagcaatg gaaggttcat
    12841 tgaaattaaa agagatttca tatatacatg cagaagcttt tgcagcagga gagctaaagc
    12901 atggaacaat agctttaata gaaaaaggaa ctcctgtaat tgccatagct actcaagaaa
    12961 agttatttga aaaaatggtt tctaatatgg aagaagtaag agcaagagga gcttatgtag
    13021 tagcaatagc tcaaagccat aataaggatg ttgagaaagc tgctgataaa ataatctata
    13081 taccaaattc agatgatatt ttatcaccaa tattagcagt tgtaccaatg caattacttg
    13141 cgtatcatgt ctctgtacta agaggatgcg atgtagataa gccaagaaat ctagctaaat
    13201 cagtaacagt tgaataaaaa cagattaaaa attaaataga taatatttat tgaaaaaaac
    13261 tcatcctgtt atttgtattt tataggatga gttttttaga taaatattaa atttgttttg
    13321 tttttgctat aataaatttt ataaaaagat tgatattaaa tcatctgtaa aatgataaat
    13381 atatagacca taccaggtat agggaacgat aaatatagtt catataaaat atagtgagta
    13441 cttaataaac atatagtcaa tatcagacat aaaaaaataa tagcttatga gaaaaattaa
    13501 aatacaaggt attagtaacc ttgtgataag ataaagtgag tagtttaaaa taagaatata
    13561 aggaactatt tataaaagaa tataatgaag ctaatagagt ataagaaaat tactttattt
    13621 tagtaaaata atttcaaata aaaatttttt taaattggga gtataaatta tgacaaatga
    13681 ttttaaaaaa gaaaagaaag aagattattt tgttaatctt aaggcaatat ctactgaagt
    13741 tttacaagaa aaaatgcaag ataacgcttg ggttatagtt gatactcgct taaatgatgc
    13801 ttataatggc tggaagttag atggagtaaa aagaggtggg catataaaaa gagcagtaga
    13861 tttttctgct aattggttaa atgtatactc agatagaaaa gatgaagtgc tagaacaagc
    13921 attgaaaact aaaagaattg atttagataa aaatatagta ttgtatgatg caaatggaaa
    13981 agatgctttg gttgtagcag attatttatc taaaaaagga tataaatatc tatataaata
    14041 tgatattaaa caatgggcag acgatgaaaa tcttccaatg gaaagatata aaaattatca
    14101 aatgatagtt cctgctttta taataaaaga tatattagat ggaaaaattc ctgagacatt
    14161 tgaagattca aagaatataa aaatgattga agcgagttgg ggagaagaat cgtatacaaa
    14221 agggcatata cctacaagtg ttcatgtaaa tactgatatt atagaacctc ctccaacatg
    14281 gatgcttgat aatgatgata acttaactaa gtttgcttta gactatggac taacaaagga
    14341 tgatactgta atagtttcaa gtattacacc tatggcatct tatagactag ctgttatatt
    14401 gagatatata ggagtaaaag atgtaagagt tttgaatggt ggaacaaact catggttatc
    14461 agcaggatat gaattagaat ttattagtaa tccaaaacat tcatgtacta attttggagc
    14521 agatatacca gttaactcac aattagttgt tactacatct gaattaagac aaaaactaaa
    14581 agaaaaaaat aagtttatac tagtagataa tcgtacttgg gatgaacata taggaaaagt
    14641 atctggatat acatattatg ataaaaaagg gcgtatacca ggagctttat atggacattc
    14701 aggtagtaat agtgtttcac tagaagaata tcgtaatatt gataatacaa tgagaaataa
    14761 atctgagatt ttagaaatgt gggacaagga aaatatagat gtgaataaac agcttatatt
    14821 tatgtgtgga agtggatggc gtgctgctga agtattaaca tatgcaaatg tcataggagt
    14881 ggaaaatacc tccctttata gtgatggatg gatgggctgg agtcttgata attcaaattt
    14941 aattgaagtg ggagaacaca aataaagttt ttgaaagtac aatagagttg tttaaaacaa
    15001 aggtgttttt gatttattag gaagagtgtg taaattgtgt attgggaagt gtacttaggt
    15061 atgttcattt ggagctatgg aaattgataa aaaagtatta tctgtttatt ttgctggtaa
    15121 aatatgaata gattatagat tatagattat agattataga ttatagatta tagatttaag
    15181 tatagactaa gtaatttata ttacagaaga taatatataa gaattaatgc aagatgtatt
    15241 tgatacatat aaggaattgg caagtccagg agaaggatag agaaaattga gattgaagag
    15301 tttgaaagag attattagat gaaaaataca aaaatgtata aaaatacata ttgagaaagt
    15361 atatctctct taaatttgtt aataattcta ttatacagaa aaacatattt aagggggatt
    15421 ttttactatg aaaaaagtaa aattagctat aagctttttt atattaatga ttataggaat
    15481 gacaatatct tatgcagata caagtataaa tactagatac aatagattaa gtaaagctct
    15541 tgaaatatca gaagcaaatt ttaaatttta taatgttaaa attaattctg ttataaatca
    15601 taatttatca aataaagaat ttcataatat ttttatagaa ataattaaca atctaaagtt
    15661 ggataaaaaa ggaataaatt ggaatcaaaa agggtctggc gataaaatac aattatgtgc
    15721 tactataaaa ggtagcgata aagaaatttc tataaaagga ataaataaaa ataacaaaga
    15781 aagttacata gtagttgaca tattggataa caaagtatat aaacataaag aggttattta
    15841 tagtaacgta gaaaatgtgt taaataaata ttcatctcaa gtagatgtgt ctgcttgtat
    15901 tgttggagaa tacacaaaaa ggttacaaaa caacaaatat gatgatattt tacaaaaaat
    15961 attatataat atgagtgcta agcaaataga tgaagttaaa gacgtaaact ttttatctat
    16021 aacagcatat agtaaagtat taaatgatta tgaattagaa tatttagggg aaaaaattaa
    16081 tttaaatata ggaattaaat ataatgagga tgacggaaaa acactcatat atattgccac
    16141 accaattata aaattagatt attaattatg aggagatcaa aaaatatggc taagataata
    16201 gtaaaaaaga gtaatccact taaaggtagt gttaaaatag atggtgcaaa aaatgcagtt
    16261 ttaccaataa tagcagcgac tttattagca aacggaaaat caacattaaa tggagtacca
    16321 aatttaagag atgttcatgt tatatcagat ttattaagac atgtaggagc agaagttgaa
    16381 tataaagaaa atacacttac tgttgatgca agcaatataa aaacttgtga agctccatat
    16441 gagcttgtaa gaaagatgag agcttctttt ttggttatgg gaccattgct tgctagattt
    16501 aatagtacga aaatttctat gccaggagga tgtgctatag gaacaagacc tatagattta
    16561 catttaaaag gatttaaagc cttaggtgca aagatagaaa tggaccatgg ttttgttgaa
    16621 gcagcaacag aaaagttagt aggaaataaa ttatatttag atttcccatc tgttggagca
    16681 actgaaaaca taatgatggc agcttcttta gcagaaggga ctacaataat agagaatgca
    16741 gcagaagagc cagaaatagt agatttagca aacttcttga acgaaatggg agcagatgtt
    16801 aaaggtgcag gtactaatac tataaaaata aaaggtgtta aagagttaaa aggagcagaa
    16861 cataatgtaa ttccagacag aatagaagca gcaacttata tggttgcagc agctatgaca
    16921 aaaggtgata taactgttga aaatgtttta atggagcatt taaaaccagt tgtagctaaa
    16981 ttaagagaag ctggttgcga aataacagaa atggacaatt ctgtaagagt tgtaggtcca
    17041 aaagtattaa aaccaataga tataaaaact ttaccacatc caggatttcc tacagatgtt
    17101 caagcacaat ttatggctat gcttactgta gccaatggaa ctggtgtagt tatagaaact
    17161 gtttttgaaa atagatttat gcatgttgct gaatttaata gaatgggtgc taatataaaa
    17221 atagatggaa gaagtgcggt tgttaatggt gtagatgagc tacacggtgc agcagtaaac
    17281 gctactgatt taagagcggg ggcagccctt attttatgcg gtcttatagc agaaggagaa
    17341 actcaaatag gagaaatata ccatatccaa agaggttatg ttgatataga taagaagata
    17401 acagcactag gtggtcaaat cgaaatagta gaagattaat aattggttta tagaagaaag
    17461 ttataaatta atagggttgt tcatatactt aaatacaagg ttttaaaagc taaggaggag
    17521 agtatatgaa gaacccattg gttgttttgt taggatttgt gacttgctct gtgttagttc
    17581 catcacttat aactttagtt tcttataaaa atgtagaatt aactgaaaaa ccagagagcc
    17641 cagtatctat aaacaaaact attaaaaaaa gtgatattga agacaaaggt aataaagaag
    17701 aaaaaagtac agtgagctat gaaactgtaa ataaaaaagc accaattatc aatgtatata
    17761 atcatataac agggaaaacc gaaaagatgg acatggagaa ctatttatgt ggagtactag
    17821 ctggtgagat gtcttcagag tttgatatag aagctttaaa agctcaatct gtagcagcta
    17881 gaacatatgt agtatataaa caggaacatg gtaaatctag taaacataaa aatgcagtag
    17941 tatgtactga ttataaacat tgtcaagaat ataagagtta tgatactctt aaaaaattaa
    18001 atggtgaaga atggattaag aataaatatt ccaaaatcca agaagcagtt agaggaacta
    18061 aaggtcaaat aataacttat aatgataagg cgatacttcc tctttatttt tctacatctt
    18121 caggaaaaac agaaaatagt gaagaagtat tttctgcaaa atatccttat ttaaagtctg
    18181 tggaaagtcc ttatgataag tattccccta aatttgcgtc aacacttaaa ataagtaaca
    18241 cagattttgt taaaagtctt agaagggcat atagcactat tgtgatagat gtgaataatt
    18301 taagtaaaca agtttcaata actaaaagaa gtgatgccgg aactgtagaa aaaataaaat
    18361 taggaaataa agaattgact ggaaaagaca ttagaactgt ttttaaattg aactctgcaa
    18421 actttgacat aaaatttgga gaaggataca tagatttcgt ggtaaaagga tatggtcatg
    18481 gtgttggtat gagccagtgg ggagcagaag gaatggcaga agagggctat aaatattatg
    18541 atatattatc acattactac acagatacaa aaataaaaga tatatactaa taattgtata
    18601 aaatgccaaa aacacgtcaa taatccaaat gtagaaatat atttggaggt gcaaaatgaa
    18661 gaaaaagctg ttagaaaaag atggttttta tttatctttg tttgtatgtg tttgtttatt
    18721 agcagtaggt ggagtttggt ttacaaataa taatgtagat aaattggctt ctaataaagg
    18781 aattatggaa aatgctaata aagatagtga agaggaaata catttaattg aaaaagacaa
    18841 gaaagatgct atccctactg caacggattc aaaacaaaac ttagaaaaag ctaagtcaaa
    18901 agaggaaaat aaatcgagta caacaaaatt aaattacatt ggagataaag taataagagg
    18961 atattcagaa aaagaaccta gttattctaa aactcttgat gtttgggaaa ctcataaagg
    19021 tgtagatatt agttgtacta aaggcaaaga agtaaaatct ctattaaacg gaatagtagt
    19081 agatgtattt aatgatgaag aatatgggca atcagtcaaa ataaaaagtg acaataacat
    19141 tgtagtagta tattcaaatc tagataaaaa tgttagtgtt aagaaagaac agaaggtaac
    19201 agaaggacaa tctctaggta ctgtaggtag tacttctcaa atagaaagtg aagaaggtat
    19261 acatgttcat ttagaggcgt atagtggaga aaaatctata gacccaatga gtctaattaa
    19321 gtaaaagtat aacaagttta atcatatttg gttaagcttg ttatattttt tggtgttttt
    19381 acatatatat cattaaagct attatgtgag gggggtaatc tttgagatct catatagagg
    19441 aaagggccat agtggtagca aaatatatac ttgaaaaaaa cactacagta agacaaaccg
    19501 ccaagacatt tggagtaagt aaaagtacaa ttcataagga tgttacagaa agattgaagg
    19561 aaataaatcc atcccttgcc aaagaagtta aaaatgtgct ggacaaaaat aaatcagaga
    19621 gacatattag agggggatta gcaacgaaat taaaatatga gaaggaacat aaaaaaatgt
    19681 aggttaaaaa cctacttttt ttattgaatt aaattataaa aagaaatata ttaagtaatt
    19741 ataaatagca aaataataat actctcaaaa gatttatttt ctcttgatta aagcgagatt
    19801 tttgtatatg ctttattata ggagtattaa aataggctta taatataatc taatattata
    19861 aataaaggag ttgaaaaaat ggctggagct gatataggca tagatttagg tactgcaaat
    19921 gtgcttgttt atgttagtgg taagggaatc gttttagaag aaccatctgt tgtggcgata
    19981 gacaaaagaa cagattctgt tttagctgtg ggagaagaag ctaaaaagat gatagggaga
    20041 acaccaggta atatagtcgc tataagacct ttaagagatg gtgtaatttc agattatgat
    20101 gtaactgaga agatgttaaa atcttttata gataagatag ttgataaaaa gggatttgga
    20161 agatttttta tgccaagaat tatggtatgt gtaccaacag gtgtcacaga agtggaaaaa
    20221 agagcagtag aagatgctac gagacaagct ggagctagag aagtatatat catagaagaa
    20281 ccaatagcag ctgccatagg agctggtgta gacatatcac agcctaatgg taatatgatt
    20341 atagatattg gtggaggtac tgtagatata gctgtaatat ctctaggagg agctgttgta
    20401 agtgaatcta taaaggttgg tggagataga tttgatgatg ctatagttaa atatatgaaa
    20461 aaacaacaca atcttcttat aggagaaaga acagcagaga aaatcaagtt tgaaataggt
    20521 tctgcattta agagagaaga agaaaaatat atgaagataa caggaagaaa tttaattaca
    20581 ggacttccta gttctataac tataaattca acagaaatgt tggaagcact tagagaatgt
    20641 gttgagcaaa tagtagttgc tactcatgct gttttggaaa aaacgcctcc tgagttagca
    20701 gcagatatag gagatgtcgg tataataatg actggaggag gttcattatt atatggatta
    20761 gataaaataa tagaacagag gacaggaata actgttacaa tagcagatga accattatct
    20821 tgtgttgcaa aaggtacagg aatagcttta ggttcaatag acttacttga aactggtggg
    20881 tctttcaaaa gaaaataatt aggagagttt agaatgttaa atatagatga aataaaaaaa
    20941 ttaataccac atagatatcc atttttatta gtagacaaga ttacagagct agaagttggg
    21001 aaaagagctg ttggtataaa gaatgtaaca gttaatgaac catttttcca aggtcacttt
    21061 ccagaatatc ctttgatgcc aggagtacta atagtagaag ctctagcaca agtatgtgga
    21121 gtggcaatga tgtctgtaga ggaaaacaaa ggaaaactag gtgtatttgc aggtatagat
    21181 aaagttagaa tcaagagaga agtaagacca ggagatacat taactatgga aattgaaatg
    21241 acaactctta gaaaaaatat agctaaggca gatgctaagg catatgtagg tgaagaactt
    21301 gtctgtaaag gtgaattaat gtttgcttta gtagaaaaat aaaaacttga aatataaaag
    21361 taaaaaggta gataatttgg agtatttaat cagatactct aaaatatcta cctttttata
    21421 taaagaattt tatcatttag tttatattta gattaggtgg atactaataa agccaaggct
    21481 attgtttcag ctaaatctaa tataaagcta aggcgtatat tattaaatga aagtttatta
    21541 ttttcatcaa ttttatctac aattcctact atagaataat ttcctatttg aggtagtttt
    21601 ttaccaacac cttttccggg caggataggt ccttctctaa tctgtatatt acctatgtta
    21661 ctctgcgacc ccaagcaagc atctatagcc aagaagttac cttgtatatg tgtatttttt
    21721 atagtatcta aagactcata tatatttaag gcgtgaatag ggttatctag tgtaccgtaa
    21781 acggggtatt taaagttact gttcttaagt attgttccaa ctaaagggcc taaagtatca
    21841 cctatagccc tgtctgttcc tatgcaaaca acaatcgtat tttcattaat tatagttttt
    21901 agaactgaac ttagcatctg tataacatca tcatcgctgt agtttacttt agctaaatac
    21961 aataggaaca cccccttcaa aaaaatatat gaataaaaag cattaaaaag acctaacttt
    22021 aaatttcaga tagatagaaa attttatttg tcttacaaaa aaaggaatag aaaaaatatg
    22081 actaaaaata ttgacaaaat tttaaaaaaa tagtaagcta gtaaagtaaa acaaaactta
    22141 aatcttgatt accttatcaa gagaggctga gggataggcc ctgtgaagcc cagcaaccac
    22201 ccaaatggga aaaggtgcta attcctacaa gacaaaagtc ttgaaagatg aggtcagtta
    22261 attagtatgt aattacgatt attaacctct tcttataaag gagaggtttt tttattttga
    22321 gtaaatttct aaaattaaaa ataaggtaaa acctataata aaaattaaga ataaataagg
    22381 gggaataaaa atggcaagac atttatttac gtcagaatca gttacagaag gacatcctga
    22441 taagatatgt gaccaaatat cagattcaat attagatgca ttattagaaa aagacccaca
    22501 atctagagta gcttgtgaaa ctacagttac tactggttta gttttagtag caggagaaat
    22561 aagtacatct gcttatgtag acattcctaa attagtaaga gaaacagtaa gagaaattgg
    22621 atatacaaga gcaaaatatg gatttgattg tgatacttgc gcagttataa cttctattga
    22681 tgaacaatct ggagatatag ctatgggagt tgatgaaggt ctagaaagta agactggaga
    22741 agagatagaa gaagaaatag aaaaagttgg agctggagac caaggtataa tgtttggatt
    22801 tgcatgtaat gagactccag aattaatgcc acttccgata tctttggcac ataaattatc
    22861 aagaaggctt acagaagtta gaaaaacagg tttagttgat tatttaagac cagatggaaa
    22921 aactcaagtt actgttgaat atgaaggaag taaagctgta agagtacata cagttcttat
    22981 atcagctcaa cattgtgaaa cagtatcaaa tgataaaata agagaagatt taattaatca
    23041 tgttattaag gaagttatac cagcagaatt acttgatgaa gagactaaaa tttatataaa
    23101 tccaacagga agattcgtta taggaggacc acaaggagat acaggtctta caggaagaaa
    23161 aataataata gatacttatg gtggatattc tagacatggt ggaggagctt tctcaggaaa
    23221 agaccctaca aaagttgata gatctgctgc ttatgcagct agatatgttg caaaaaatat
    23281 tgttgcggct ggtcttgcag acaaatgtga gatagaactt gcatatgcta taggtatagc
    23341 tagaccatta tctatattta tagatacttt tgggacaggc aaggtttcag aagaaaaact
    23401 tgttgaatta gtaaataaac actttgattt aagaccggga gcgataataa gagacttaga
    23461 tttaagaaag cctctttata aaaaggttgc tgcttatggt cactttggta gaactgatat
    23521 agatttacct tgggaaagaa ctgacaaggt tgagcagtta agaaaagatg ctttaggtga
    23581 ataattttta actaaaaagc tgttagaatg taattttacg attactttct aacagttttt
    23641 ttattatgag ttaaatattt ttaataaatg ttaatttatc atcatataat gaataaacag
    23701 tatttaatct ggtcaataca ttttatataa tactctacta aatttataca tattatggga
    23761 tatatgttaa aggatggtta agaatgtgtt aactaatgtt aaataattaa aaatatattg
    23821 aaatatcaca ataatgagtc taatataatc ttaataaact tacactttaa aaataatttt
    23881 aattgggggg gagtagtgta tgtcaggcaa agtaaatatt aaaacggtta taagttttgc
    23941 tggagcttat gttgctactg taataggttc tggatttgca actggtcaag aaatactgca
    24001 attttttacg ttttatggat atgcgggtat tataggtgga ataatctcta tggtactatt
    24061 ttcatggttt ggagcagaag ttatagataa gggaagagaa ttgaaactta aggagccaat
    24121 aaaaatttat caagtatatt gtggaaaata tcttggaaca ttttttgaat ggtttggtcc
    24181 attgttttta tttggaacat tagtaataat gattgcagga gcaggagcta cactatcaga
    24241 gtactatgga ttaaatccat atgtaggtag gattggtatg gcaatcgttt ccctaataac
    24301 tgtttcatta ggtttgacaa ggctttctaa aatactaggt aatataggac ctataataat
    24361 aatatttaca cttttagtag gagcaattag tttatttagc aatattgatg ctttatcaaa
    24421 tgctggaaat atggttgata gtttaaatat taaaactgct acatcaaatg gatatttctc
    24481 gggagtactt tatacttgtt acaatgtaat aatagtaata acattcttga caggaatggg
    24541 agcttctgca gtaaataaga aagatgctgt atggggtggt atagtaggtg gagtagcact
    24601 tatggcagca gctataatga tgaatttggc attactttca aacataggaa atatatacac
    24661 aaaagagata ccagcacttt acttagctga taagatatct ccaataatag gtattttatt
    24721 ctcagtagta cttttactag gaatatacac tacagctgta ccattgttat ggtcagtaac
    24781 taaccgattt gtagaagatg accaccctaa atttaaaatt ataacaatag tggtaagtat
    24841 attagcttgt ataggaggtt tactgccatt tgataagtta gtaggtacac tatatccata
    24901 tacaggttac atgggaattt taattttact atgtatgctt tatagaagaa ttactaaaac
    24961 tgaggggtac aaggagaata aatctgaaat aagttagtac tgtaatatac ttatggaatt
    25021 taatttatac tctaaatata gagtaattaa aataaaatag ctatttaaat atggtacctg
    25081 tatttcatgg taccatattt tatggatgca gatttttttt gtttctgata aaatgtataa
    25141 tagttggaaa ttacgttaag tataaaaata taaaactttt ctacgattat catagtttta
    25201 atatataaaa ttatactaac atttaaatgg agcgattatt agtattttta ggagataaaa
    25261 atggaaaaat tagaaggaat gataagtgaa atagtcttta aaaacgaaga taatggttat
    25321 acaatagctc acctggtaaa tgaaaatgat gaaattgtgg ttgtaggatg tatgcctact
    25381 ttggcaattg gagaaagcat agaagttgaa ggaaagtggg taaatcataa gatttatggg
    25441 actcaatttg aagtgaatag ttttatgcca gtaacgccat catccttaga aggaatttat
    25501 gtttacttat cttctggaat gatacatggt attggtgaaa aaatggcaaa aagaataatt
    25561 gataagtttg gtgtagatac gttagaggtg atacaaaatt caccagaaaa attacaagaa
    25621 gtagaaggta tagggagtaa aaaagtaaaa caaatagtaa aaagttatga agaagacaga
    25681 gaattgagaa atataataat acagctatct ccttttggaa taacaccaaa ttactgccta
    25741 aaaatatata aaaaatataa aagcagtgca atagaagtta taaataaaaa cccataccaa
    25801 cttgctgaag atataagagg aattggattt aaagtagctg atagtatagc aagtaaaatt
    25861 ggtatagata aaaactctaa agatagaatt tgtcagggaa tcttatacac tttaaataaa
    25921 tctttaagta atgggcatac atatttacca gagcatgttc taatacaaga ttcggaaaaa
    25981 ttattggagc taaatggaga aataataaaa gagtgtgtaa tgatgctagt ttataatcaa
    26041 aaaatacata tagagaaagt aaacaatgaa aatcttatat accttatgcc atactattta
    26101 gctgaaaatg gagtatgtag tcaaatagta aaattatcac agtatgaatt tgaagactta
    26161 aaaatagata tagataacga gattaatgtt ttagaagaag ataagaaaat taagttagca
    26221 gaaaaacaaa ttttagcagt caaagaatct gttaatagtg gagtattaat tataactggt
    26281 ggaccaggaa ctggtaaaac aactaccatt aatgctatca tagatatatt tgaaaataat
    26341 ggtaaaagtg tgactttggc agctccaacg ggaagagcag caaagagaat gagtgaaact
    26401 tctaataaag aagcaaaaac tatacatagg ttgctagaaa tgggattttc aacagatgat
    26461 gatttaactt tttttaagga tgaagaagac ccaataaatt cagatgtaat aattgttgat
    26521 gaggtatcaa tggtagacat tatcttaatg tacaatttac ttagagctat aaagttagga
    26581 acaagagtaa ttttagttgg agatagtgac caattacctt ctgtaggtgc tggaaatgta
    26641 cttaaggata tgattaattc taatattata aatgtagtta aattaaatga aatatttaga
    26701 caagcgcaag aaagtatgat aattgtaaat gcccataaga taaacaatgg agaaccactt
    26761 tatctaaata caaaaggtaa agatttcttt tttataagaa aatcaactaa tgaagaaatt
    26821 ttaaatgaaa taataggatt ggttaatgaa agattaccta aattttataa agttgataaa
    26881 ttaaaagata tacaagtttt atcttcaatg agaaaaggtg aattgggagt tactaatctc
    26941 aatatagagt tacagaagta tcttaacaaa aaagaaaaat ttaaagttga agaaagtttt
    27001 tctaaaagat tatttagagt tggcgataag gtaatgcaag taaaaaataa ttatacaaaa
    27061 aaatgggaaa cagaagacca aaaagaaagt ggagaaggta tctataatgg agatatagga
    27121 tatgtttatc acatagataa agataagaaa actatttata tattatttga ccagactaaa
    27181 attgtatcat acctatatga tgaattagat gaaattgacc atagtttttg tacaacaata
    27241 cataaaagcc agggaagtga atttccagtt gtagtattgc caatagcgtg ggctcctcct
    27301 atgttactta gccgtaattt attatataca gcagtaacaa gagcaaaaaa attagttgta
    27361 ttagttggag atgtgaaata tcttgaatat atgataaaaa ataatagagt taatcaaagg
    27421 tattcaaatt taggatataa gctaaataaa tttaaacaag aagggttgtt gatagaataa
    27481 atatgaaaat atgcaatgat aattttatta agaattttaa aaatatgatt aataaatgtt
    27541 tggattttat atatcctgag aatataagtt gcataatttg tgataaatcc ataaagaaaa
    27601 caaatactta ttcaatatgt aaaagttgtt ttaaagaaat gaattttata caagatggtt
    27661 gcatgaagtg tggaaaacca ataatacgtc attcaattga aaaagaattt attgaagagt
    27721 gtagttattg ttttaataaa gacttttatt ttgataaatc aatatcttgt attgaatata
    27781 atgatgtgac aaaaaagatg gtgttagggc tgaagtataa ccaaaaaacc tttatggcta
    27841 agtatatagc tcaaataatg aaagaaaaat tatatttaga aaacataaaa tttgattata
    27901 tattatttgt tccacttcat aaaaaaaggc taaataaaag aggttttaat caggcacaaa
    27961 aaatagcatt taatttaagt aaaatagtaa atattccatt attagattgc ataagtagaa
    28021 aaaaatatac aagaatgcta tataaattga ataagaaaga acgaaaagaa gaattaaaaa
    28081 atgtatttgt tgttaaagaa aatgttaaat taataaataa taaaaatata cttttaatag
    28141 atgacatatt tactactggt ctaacaacaa acgaaatctc taaactttta aagttatcag
    28201 gtgctaataa ggtttttgta ttgactctac ttacaaaagc aaatgataat tatattatgg
    28261 aataatctta tatattgaca attttagttt aaaatattaa aatgaataag tgatatttag
    28321 gtctgtggtt gaaagtccaa gccagtcgca ggcaaaggga tccacgtaag ttaacactaa
    28381 aaattgggtt aatgatcatg gtgcggctta gaagtaagac ctaccgataa taaaatcgag
    28441 agagagtagt agtgcggcga acccaagcga accctccagt aggcgagtgt ggggtcaaaa
    28501 accaggtcag ctaaatatca tattaaaaga gagctagcat acctttatgt atagctcttt
    28561 tttatatata ataaatttag gttgagtcat aaagaggtga tatgtttgaa taaaagacaa
    28621 gaaaatttat taacaacatt gctattaaaa gaagagttta taaatgttaa taagattgcc
    28681 aatgattttg agtgttcaga aaggacaata aggaatgatt gcaaatatat tgatgaatgg
    28741 ttatctactt tttgtaatgc ttatatcaaa agaaaaccta atattggaat taaatttagt
    28801 ggagatggaa cagacaaaat actggtaaat cagaagttgc gaggtgaagt taaaagagta
    28861 aatagtgagc ttcataagca agttgagata ttaaatttac tactttgtaa taataaaaaa
    28921 gttactttaa atgatttatc taataagttg tacataaata aaaatattgt cagagaagaa
    28981 ataagtaaat tatcagtagc attaaataat tataatttaa atgtctcgac taaaaaaggc
    29041 agtggtatat atattgaagg ggatgagaaa gacataagaa taatgttagt atcattctta
    29101 tttaaataca tagataaata cagattaaac atagatgaaa tagagtactt ccatatagta
    29161 gatataataa ttgtaaaaaa cataatgctt actatagagg aggatatgga tataagactt
    29221 acagatatat cattcaagca attgatgcta tttttgctta taaatataat acgaataagg
    29281 ctgggaaatt ctttaaaagt atcaaatact aagaaagagc ttaagctaaa aatgcttata
    29341 ttggatatag aagatgaatt gaaatctaat ttatccatag tattaaatta tgaagaaaaa
    29401 ttgtttatag aatctttaat attgggtatg aacaaacaaa taaatagtaa taatattaag
    29461 aaatctttat atctaaatga agaattagca agttatacaa aaaaaattat aaatttagta
    29521 tcagaagaat caggaataaa ttttaataat gatatattac tttatgaaca attaatatgt
    29581 catttgaatg ttacaatgca tcaaatgaaa agcaatgttt acttagaaaa tccactttta
    29641 gataacataa aaactaaatt ttgtttttta tttacagtaa tatcaagttc aatagaaatt
    29701 gaattaaaag gaaaacaaat aacggaagat gaaattggtt atcttacact tcactttcaa
    29761 acctctctag aaagaaaata cagcaaaagg gaatcaaata aaaaggcaag tattgtatgt
    29821 ccatttagtt ttggagtaag tatgctatta aaagtaaaga tagaaaagag atttgataac
    29881 ataaaaatta ttgaaacatt aagagaggaa gatttaaaaa gagataactt taataagact
    29941 atagatttta taattgcatt tcaagagtat gaaaatatcc aaaaaccaat ttttataaca
    30001 acaccattat ttactgatga ggatgagaat aaacttaaag agtttacaaa caaaataaaa
    30061 gaaaaagaca cctcatataa acttatgaac agattaatga tgtctgatta tttaataaga
    30121 gagttagaac atgatgatat ttatgaagct ataacatatt taactaattt attaattgaa
    30181 aaacattatg cagaaaaaga gtatttacaa tctattataa caagagaaaa gtcatatcca
    30241 accaatgttg gaaaaggaat attattccct catggagaca tgaaatatat aaaacaatct
    30301 gttttatgtt ttgcaaggtt aaaaacacca ataaaaatta gaaatggaaa ggatataaag
    30361 attatacttt tattagcata taaaaaagat gatgatagaa aagtatttag agaattattt
    30421 aaagaaatat ctaatttaac agaggatgaa aatatgctag atattctaag taaatgtgac
    30481 attgaaaagg taaaagatat tcttatataa aattcataat gaactatgga taagcacaaa
    30541 attttccatg tgatggaaaa agttgtgcat gtttttttgt ttttataaga tattatatgt
    30601 ttgtaaggta taaataatct aaaagtagag aagcaattta ggaggaaaaa atggaagaaa
    30661 tagttttaaa gattataatt catgctggaa atgcaaaatc tatgctttat gaagcacttg
    30721 actatgcaaa ggaaaacgat tttaaaaaag cagatgaatt aattgaaaat gcaaatgaag
    30781 aaattttaaa agcacataaa gtacaaactg aacttataca aaaggaagct gggggagata
    30841 aatcagatat atctatttta ttgattcact ctcaagacca tttaatgact tgtatgagtg
    30901 agagaaacct tataaaagaa atgattatgt taagaaaaga aattcaaaaa ttaaaataaa
    30961 aaaattaagg gggatttaaa agaatgaaaa gaaaagtata tttattttgt agctttggta
    31021 tgtcaacaag tttacttgca gataaaatgc agaaggtagc agatgaacac aatttaccaa
    31081 tagaagtaga ggcatttccg atagcagaaa ttgacaaaat agttgaagaa aaaaatccag
    31141 attgtatatt attaggacca caagttaaat acatgctaaa ggagttaaaa ccaaagtttg
    31201 aagctcaagg aaaacttata gatataatca atgaagttga ttatggaact atgaatgggg
    31261 aaaaagtatt aaaacttgct ataaagctaa taaaatctaa acaagcgtag aattatagta
    31321 aaaaataata ggaaatgagg gtggatagat gaatagattg gaaagaatac ttatgccatt
    31381 ggctgaaaaa ataggtaaaa ataaattatt aattgcaatt agagatggtt ttttaatatc
    31441 atcaccatta ttaataatag gttcattatt tttattagta gcaaattttc ctataaaagg
    31501 atggaatgaa tttgtaggtc aaatttttgg tccagattgg gctgtaaaat tgcaacaacc
    31561 aactgtagca acatttgaag taatgacttt acttgcagtt ttaggaattg gatattctta
    31621 tgcaaagcaa atagatgtag atccaatagc atcagctgca gttgcaatag ttgccttttt
    31681 tatagtaaca ccgtttgtaa taccatatac accagaagga acagaatcag tctacatggt
    31741 aacaggtata ccactaggtt ggatgggctc taagggaatg tttgtaggta tgataacagc
    31801 aataacctct gtaaaattat ttgctgcagt tgtaaaaaaa ggatggacat tgaaaatgcc
    31861 agatggagtt cctccaaccg tatcaaaatc atttgcagca ttaataccaa gtgcaatagt
    31921 tatggtagtt ttctttttag ttaagatagc ttttgaagct acaccatatg gaagtataca
    31981 tgaatttata tttaagtttt tacaaatgcc attattaaaa ttaggaaata gtttaggtgc
    32041 tatggcaatc gcttatatat tcttacattt attctggttc tttggaataa atggaagtag
    32101 tgttgttggg gctgtgtata atccaatatt aaaaattcta tcagctgaaa acttagctgc
    32161 ttttcaggct ggttcaaaaa taccaaacat aataacaggt caattccaag atatgtttgc
    32221 gacatttggt ggagcaggta gtacactatc attagtcata gctatgttat taatatgtaa
    32281 aagtaaacgt ataaagtcaa tgggaaaact atcaatttta ccaggaatat ttggtataaa
    32341 tgagccattg atatttggtc taccaataat gcttaaccca atactattga taccatttgc
    32401 aatagttcca acaataaata taataatagc ttacttctgt atgtcagcag gattagtgcc
    32461 tcttacaaat ggagttcagt taccgtggac aactccaatt atattctctg gttttttaac
    32521 aacaggatgg caaggtgcag tcttacaatt agtgcttctt atactaggaa tatttatgta
    32581 tataccattt ataaaaatgc tagataagca atacttgaga gaagaaaatg aaactgttga
    32641 agaggaagat gatgatatat cttttgatga tttagtatta taggaggaag attatgaaga
    32701 aaatagttat gatattggat caaatacaag ctggagctgg aggaaaagag aaaagtaata
    32761 tacctcctgc tggaaaatca agcccattag gaccaggtgt aatgatggag caatttttaa
    32821 atgaatctaa ggtaatagct accttatttt gtggggatga attttttgta aataatgaaa
    32881 aagaagtaac gagtaagatg atagctatgg taaaaaaatt aaaccctgat gtagtaatct
    32941 gtggaccaag ctttaattac gaaaactttt ctaagatgtc agcaatactg tctaaaaata
    33001 taaatgataa aacagatata ccagcttttg cagctatgtc agaagaaaat atagatgtga
    33061 taaacgaata taaaaatgac atatgtatag ttaagactcc caaaaaaggt ggaattggtc
    33121 ttaatgattc attaaataac atatgtaaat tggcaaaagc aatagcgaat aaagaagata
    33181 taactttgat gaaagaagaa ttttgttatt aataagagta ttaattatta gcaagtttaa
    33241 atattatact atttataata agcatacatt agtaaaatct atataattta aggaggggaa
    33301 ttttaacatg tggggaatga tagcgacatg gagaatggcc ctagaaggaa taactgaggc
    33361 atcagaacat ttagaaaaag gcatggaagc aggtgatgct gtagagcttg ctgtaagaag
    33421 agttgaagac tatccattct ataaatcggt tggatatggt ggacttccaa atgaaaattg
    33481 tgaggtagaa cttgacgctg catttatgga tggtaacaca ttagatattg gcgcagttgc
    33541 tggtataaga gattattcta atcctgtcag catagctagg aagttaagtt atgagaaggt
    33601 aaataatttt ttagttggaa taggtgctga agattatgct cataaaaatg gatttgaaag
    33661 aaaaaatatg ttgactgata gagcgaagtt acattataaa aagagaaaaa aagagacttt
    33721 agacaaagga ttaagcccat atgctggtca tgatactgtt ggtatgatat ctttagacaa
    33781 aactggaaaa atgtgtgcag ctacatcaac aagtggattg tttatgaaga aaagaggtag
    33841 aataggagat tctgcactat ctggttcagg attctatgtt gatagtagta taggaggagc
    33901 tacagctact ggtttgggag aagatttaat gaagggatgt atatcttatg agatagttag
    33961 gcttatgaaa gaaggaaagc atccacaaga agcatgtgat attgcagtaa atgaacttga
    34021 taaacagctt atcaaaagaa gaggaaaagc tggtgattta tcattagttg ccatgaataa
    34081 taaaggtgaa tggggagtag ctacaaatat agttgatttc actttttcag tagtaactaa
    34141 agaagaatct cctgctgtat atatttcaaa tagagtagat ggaaagacta tacataaggt
    34201 tatatcaaat gagtggatgg aagcatatga ggctagaata aaagcaccga tagaagtaag
    34261 tgagtaaagt agtgttacat aattaaaata gagttttata ataaggagat ttttatgaat
    34321 atcataagta ataaaataga tgagttaaaa gaagatttat taagtgatat tatagatatt
    34381 gtaaaaatac ctagtgtcaa aggggagtca gaaaatggct tcccttttgg cgaaaaagta
    34441 ggggaggcac ttaataaagc attagaaata tcaaaaaaat tgggatttaa ggttagaaac
    34501 ttggataatt atattggtta tgcagagcat ggagatagtg atgattatgt ttgtgtaatt
    34561 ggacatgtag atgttgtaca tgaaggagat ggttggaagc atcagcctta taagggagaa
    34621 gaaactaatg gaagaattta tggacgagga gtactagaca ataaaggacc tataatgtct
    34681 gccttatatg gattatatgc tattaaagaa ttaaacttaa aattagataa gagagttaga
    34741 ataatatttg gtacaaatga agaaagtgga tttgaagata taccatatta tttagaaaaa
    34801 gaaaaaacac ctattatggg atttacacca gattgtaaat atcctgttgt atatggtgaa
    34861 aaaggcatgg caaagattag aataaaaagt aaaatcaatt atgaagaaga tgtataccta
    34921 ggttttatag aaaatatgtc tgaaaatgtg cttgttacat ataaagagct gaatatagaa
    34981 aatagtaata ctatattaga tataaaggta aaatacgatt ttagttataa attgaaagat
    35041 gtattagatg aaataaaagc tagttttcca aatagtatcg atatagaggt aatttctaat
    35101 tttaatcctg tatattttga taaagagagt aatcttgtta aaaagcttca gctagcttat
    35161 gaaagggtta catcattaga tggaactccc gtgacaacta atggaggtac gtatgcaaaa
    35221 gtaatgccaa atatagttcc gtttggacct tcatttccag gacaaaaagg aatagctcat
    35281 aatcctgatg aatacatgga tatagaagat ataatattaa atgcaaagat atttgcaaat
    35341 gcaatatatg aattagcaaa ggagtagtta tgttagaaat aataggaatg tcagtagaag
    35401 acgctaaaat tatagaagat tgtggtgcag atagaataga gttagttagt gctttaactg
    35461 aaggtggatt gacaccaagt tttggtctta tagaaagtgt tgttaacagt gtaaagatac
    35521 ctgtgaacgt aatgataaga catcatgcga aaagttttgt atacagtaaa gaagatataa
    35581 gtataatgca aaaagatata ggtgtggtta aagagattgg tgctaatgga gttgtatttg
    35641 gtgtattgga taaaaacaat aatatagatg agaaaaatct aaatgtttta ttgaaatgtt
    35701 gtgataatct tgatgttaca tttcatagag ccatagatga atcaaataca atagatagtg
    35761 ttaaaatttt gaaggattat gataaaatta cgaatatatt aacatcagga gggaaaggaa
    35821 gtatagttga taacatacaa atgataaaga atatgatgct tagttctaat cacataaaaa
    35881 ttcttttagg aggaggtctt aattttaata atatagaaaa gataaaggaa ttaactaaag
    35941 caagtaattt tcattttggt acagcgatta gaataaataa cagtcctttt gaagatatag
    36001 atagacaaaa gcttaaacaa ctcgtaaata taatttcaag atagtatatg aatataaaat
    36061 acttagttta aataaagaga gtaagttaat tataatttgg gtatatgaat gttaataggt
    36121 attaaatatg aaattattgt tgagtatgct ttataacttt taagtagttt taaatataaa
    36181 taatgaaaga aaagatagat tatggaggtt ttagagggtt tatagttatt aaaaaattat
    36241 aaaggtatat tggcatataa tacacataat ttcctaaaat taccatctta tgacatgaat
    36301 tcacatatta ataaacaatt aatggagatt tttgtggata gaaataggag taaaaatagc
    36361 tttaaataaa caaaaacaag agttattaac agttttatcc acattatcca caggtgtttg
    36421 taataatatt aatccacata agtatcaatg taactaaaaa tgataaataa ttctatcaat
    36481 tgaatagaaa aaatcataaa agtaaataat aaaaagagga ttatgagagt tcgtgtagaa
    36541 tatattatta aagtataaat tttcttatac tgacataatt gaagggagat gtatttatga
    36601 acataattat atctggaaaa caaatcgaat taactgatgc aataaaaaat aaaatcgaaa
    36661 ctaaactaag taagttagat aattacattc atccagacac tgatgtaaaa gttacagtaa
    36721 gtgcgagaaa agcaagacaa aaaatagaag taacaatagc tactgtaaat ggtcctataa
    36781 ttagagcaga agatatgcag gaaaatttat acacagctat agatgttgta tatgataaat
    36841 taagtaaaca attaaaaaaa tataaaaaga gattacaaga taaacataaa gacaacaaga
    36901 gtataagatt ccaagattca gagataaatc tagctgatga acaagaggat tatggggatg
    36961 aaaatcaaga attagtaatt caaagacgta agaaatttag tgttaagcca atgagtgaag
    37021 aagaagctgt attacagatg gaattaatag gacatgattt ttatatgttc aagaacatag
    37081 attcagatga aatagcagta gtttataagc gtaataatgg tggatatggt ataatagaac
    37141 atgaataaag caatttagat ttaatcatat tagctatcga taaataaatt atcgatagct
    37201 ttttaaatta atattaaaag tatagttttt atggtaaaat gaataatagt acaataaaga
    37261 acgaggagag atgtaaaata tgagctttat ggataattta ttcaacatgg cagataaaaa
    37321 agaattaaaa aagttcaata aaactgttga tataatagat tcattagaac ctaaatttga
    37381 atctatggca gactcggaac ttaaaaatat gacaaatata tttaaagaaa gattagcaaa
    37441 tggtgaaagt atagatgata tacttccaga agcttttgca gtagttagag aagtttctaa
    37501 gagagtgtta ggacttagac actacagagt tcaaatgatt ggaggtatag tactacatca
    37561 aggaagaata gcagaaatga aaacaggtga aggtaaaact ttggttgcaa ctgctcctgt
    37621 ttatttgaat gcgcttactg gaaaaggtgt acatgttgta acagtaaatg attaccttgc
    37681 aaaacgtgat agagaccaaa tggcaaaaat ctatgagttt ttaggaatga gtgtaggcgt
    37741 tattatacac ggtcaaaatc caaaagttag aaaagaacaa tatgattgtg atataactta
    37801 tggtacaaat aatgaatatg gatttgacta tctaaaggac aatatggtaa tacacaaaga
    37861 gcaaagagtt caaagaggat taaattatgc tatagtggat gaggttgact ctatactaat
    37921 agatgaggct agaacaccac ttatcatatc tggacctgga gacaaatcaa ctcacttata
    37981 ttcagatgca aacacatttg ttttaacctt aaaaccagat gattatgaat tagaagaaaa
    38041 agataaggct gtatctctaa cagcatcggg tatacaaaaa gcagaagtgt attttaatgt
    38101 tgataatata acagatatat ctcatacaga attataccat catataaatc aagctttaag
    38161 agcacatgta ataatgaaga aagatgttga ttatgttgct aaagatggag aaatagtaat
    38221 agtagatgag tttacaggta gacttatgtt tggtagaaga tattcagaag gtttacatca
    38281 agcaatagag gctaaagaag gtcttaaaat acaaagagaa tctaaaacac tagctactgt
    38341 aacattccaa aactacttta gaatgtataa aaaactttct ggtatgactg gtacagcaaa
    38401 aacagaagaa gaagagttta aagcaattta taaaatggac gttttccaag tacctaccaa
    38461 taagcttatg gtaagagaag atttaccaga ttctgtatac aaaagtgaaa taggtaaatt
    38521 taatgctgta gcacaagaaa taattgaaag acataaagtt aatcagccaa tacttgtagg
    38581 tacagtatcg atagaaaaat cagaattatt atcacaaata ttaaagaaaa aaggcattaa
    38641 acatgaagta ttaaatgcta agcatcatga taaagaggca gagataatag cacaggctgg
    38701 tagattaggc gctgttacaa tagctacaaa tatggctggc cgtggtacag atatagtttt
    38761 aggtggaaac ccagatttct taactaagag agaaatgaga agaaatggat ttaaagagga
    38821 aatagtaaat agggtagata ctccaataga aggtatacct gtaaaaggta atgaaattct
    38881 atttgaagct agagaagagt atgaaaaact atttgaaaag tttaagcaac aaacacaaga
    38941 agaacaaaaa caagttgtag aagcaggtgg acttgctata ataggtacag aaagacacga
    39001 atctcgaaga atagataacc agttgagagg tcgtgctggt cgtcaaggtg acccagggag
    39061 ttcaagattc tatataggtc ttgatgatga ccttatgaga ttgtttggta gtgatagaat
    39121 atctggtatt gtagataaaa taggtcttga agaagacatg ccgatagaac atagaatatt
    39181 atctaagtct attgaaggag cacaaaagaa agtcgaaggt aaaaactttg gaataagaaa
    39241 gcatgtactt caatatgatg atgttatgaa taaacaaaga gaaataatat atgcagagag
    39301 aaaacgtgta ttagaaggtg aagatttaca agaacaaata caatcaatga ctcattctat
    39361 aatagaagaa gcagttactc tttatacaca agataaagga tttgatgaag aaggattcaa
    39421 agaacatatg tataatctgt tcttgccaaa aggtagcata gaaataccag aaatagaaaa
    39481 acttaatcca gttgaaataa cagaaaaagt ttatgaaata gctatgaaga tatatacttc
    39541 aaaagaagaa caagttggct atgagagaat gagagaagtt gagagagtaa tcttacttca
    39601 agctgttgat aaccattgga tagaccatat agatgctatg gaccaattac gccaaggtat
    39661 tggtcttcgt gctgtaggtc agcaagaccc agttattgct tataaaatgg aaggttttga
    39721 tatgtttgat gaaatgaata aacatatcaa agaagacact gtaagatatt tatttaatat
    39781 aacaatagaa actccagtgg aaagaaaagc agttgtagat gttgaaaatc tatcttctcc
    39841 atcagatgga acacttccaa caagtaaaac tgttaaaaaa gatgaaaagg ttggaagaaa
    39901 tgacttatgc ccttgtggaa gtggtaaaaa atataaaaac tgttgtggaa gataatctcg
    39961 gaggattaaa tgttaaagtt acaagagtat agacatagta tggagcagat ggcttcaaat
    40021 ttagaagaat tgagggtttc tctttgacat agcttcattg atgaaaaata tacaaattaa
    40081 tgaagaaaag atgaatcaac aagacttttg gaatgataat gaagtcgctc aaagagtact
    40141 tcaagaaaat aaatctttaa aagagacatt agaagaatat gaaagcttaa aaagcttatt
    40201 agaagatata gaagtattaa ttgaaatagg tttagaagaa gatgatgatt ctgtagaaag
    40261 agatatagaa aagtccatag aatcaatgga agaaaaatta tctgaaatga aaataaagac
    40321 tcttctaaat ggagagtatg ataaaaataa tgctatatta tctataaatg ctggtactgg
    40381 tggcttagat gctcaagatt gggctcaaat gcttttgaga atgtatataa gatggtcaga
    40441 aagtaagggt tataaagtta agcttttaga tataatatca gaccctgaag cagggataaa
    40501 gacagctaca attcttgtag aaggaacaaa tgcttatggg tatttaaaga gtgaaaaagg
    40561 agttcataga ttagtgagaa tatctccatt tgacccatca gggaaaagac atacttcatt
    40621 tgcatctata gatgtaacac cagagttaga tgaaaatata gaagtagaaa ttaatccgtc
    40681 agatttaaag atagacacat atagagcttc tggagctgga gggcaacatg taaatactac
    40741 tgattcagca gttagaataa cacatatccc tactggtgta gtagttcagt gccaaaatga
    40801 aaggtctcag catctaaata aggacagagc tatgagactt ttaatggcga aattgataga
    40861 attaaaagag ttagagcaaa aggaaaaaat agaagatata caaggcaaat actcacaaat
    40921 aacttggggt agtcaaatta ggtcatatgt ttttcaacca tataaattgg ttaaggacca
    40981 tagaacaaat gctgagtttg gaaatgtaga tagtgtaatg aatggaaata tagacttgtt
    41041 tattaatgaa tacttaaaaa tgaataaaat agtttaataa atatgcactg taattaagat
    41101 atatgtataa gaaggttagt gcttaatatg cacttacagc acttaggctg aggacgataa
    41161 ccctcagcct tcgttctgtt ttgttatact tatgaaaaac taacattagg agtgaataaa
    41221 ttggatataa atcaaatctt aaaaaaagag tttaatctta gagatgaaca aataaataat
    41281 acattaaaac ttattgatga aggaaataca ataccattta tagctagata tagaaaagaa
    41341 atgactggcg aaatgagtga tgttacacta agagaatttt atgaaaaatt aatgtatttg
    41401 agaaatcttc aaagtagaaa agatgatgta gttagactaa tagatgaaca agggaaatta
    41461 acagatgaaa taacacaaaa tatagaaaaa gcaaaaacat tacaagaagt agaagatata
    41521 tatgctccat ataaacaaaa gaaaagaact agagcaacaa tagcaaaaga aaaaggatta
    41581 gaaaatttag cattgtcaat actagagaat aatttagata atatagaaat agaggcaaag
    41641 aattatcttg atgaagagaa agaagttctt tctattgaag atgctctaaa aggtgctaga
    41701 gatataattg cagaactagt ctcagatgat gctaagatta gaaagtatat aagagaatta
    41761 gctttaagag aaggtatgat agttagcaaa agtgctactg atgagaaatc ggtttatgat
    41821 atgtattatg attataacga agcagtaaaa agtatggctc cacatagagt tttggcaata
    41881 aacagaggag aaaaagaaag cttcctaaag gttaagttag aaataaataa tgataaagtt
    41941 ttaaattaca taataaacga atatgtaaat gataaaaatt tcaaaaataa agaagaaatc
    42001 gtaagttcaa tagaagattc ttataaaaga ttaattttcc catcaataga aagagaaata
    42061 agaaatcatt taacagaaat agctcaagaa agagctataa gtgtatttgg aaagaatgtt
    42121 aaaagtttat tgcttcaacc accagttaaa gacaaagtag ttatgggatt tgaccctgca
    42181 tttagaactg gatgtaaaat agctgtagta gataaaaatg gtaaactact agattataca
    42241 acagtatatc caacagaccc tcaaaatgat gtagagggag caaagaaagt tttaaaagga
    42301 ttgattgaaa aatacgatat agatataatt tcaattggaa atggtactgc atctagagaa
    42361 tcagaaactt ttgtatcaga aatgataaaa gaaatagata gcgaagtgca gtatgtgata
    42421 gttagtgaag caggagcatc tgtttattca gcatctgaac ttgcaaatga ggaacatcca
    42481 gatataaatg tatcaataag aggagctata tctatagcta gaagattaca agacccatta
    42541 gcagagcttg taaaaataga ccctaaaagt attggtgttg gtcaatatca gcatgattta
    42601 aacaaaaaga gattagaaga agtgctagat ggagttgttg aagattctgt aaatagtgtt
    42661 ggagtagatt taaataccgc atcatactcc ctactagaac atgttgcagg aataagtaaa
    42721 gctatagcaa aaaatataat agcatataga gaagaaaatg gagattttac atcaagagca
    42781 caattgaaaa aagtaaaaag actaggacct caagcattta ctcaatgtgc tggatttatg
    42841 agaatcttag agggtaaaaa tccactagat aatacaggtg tgcatcctga aagttatgat
    42901 atatgtaaaa aaatgattga gataattgga tattctttag atgatgtaaa gaataagaat
    42961 ataggtgaaa tagatgaaaa aataaaagaa attggcttga gggaattaag tgaaaaactg
    43021 gaagtaggac aagttacgtt aaaagatatc atagctgaaa tcaaaaaacc aggaagagac
    43081 ccaagagaag aaggtataaa acctatactt agaactgatg tcttaaaaat tgaagatata
    43141 caagagggta tgactttaaa aggtacaata agaaatgttg tagactttgg tgcatttgta
    43201 gatattggaa taaaaaatga cggattagtt cataaatcag aaatgagtaa tagttttgtg
    43261 aaagacccta tgagtattgt tacagtaggt gatatagtag atgtaaaagt cattggaatt
    43321 gacttaaaca aaaaaagagt tgccttatca atgaaaaaat agataagttt atgtatggtt
    43381 atagtactta aattctatat ttgtaaatga gattactcga gtgtataaat gttaaaaatt
    43441 tgtaaaagta tatagtaaga aaatatatta aaataagaac gatagtcttg aaaatcagga
    43501 ggaaaatgaa tgatttttat aaaaaatggt aaaataaata ccataacaaa tgggataatt
    43561 catggagata tacttataga tgaaggaaaa ataatagaaa ttggagaaga tttgatagct
    43621 cctttagatg tagaggtaat agatgctagt aataaacttg tgtttcctgg atttatagat
    43681 gctcatactc acttaggttt atgggaagat ggtatagggt ttgaaggagc agatggaaat
    43741 gaagagactg acccaataac accacaatta aatcctatag atggaattaa tccaatggat
    43801 agaactttta aagaagcttt tgaaggtgga ataacttctg tttgtacaac ccctggaagt
    43861 gcaaatgtta tgggtggtca atgtatagct ataaagacat gtggaaaaag aattgataaa
    43921 atggtaataa aaaatccagt tgcatctaaa attgcttttg gagaaaatcc aaagagctgt
    43981 tatggtcagg atgataaatc tcctcaaact agaatggcta tagctgcttt acttagagaa
    44041 aacctaaaaa aagcagaaga atatttagag gatattgata tgtatgagag tcatgatgat
    44101 gaagactgtg aaaaacctga atatgatata aaaatggaaa gtttaatacc agttttaaga
    44161 agagaaattc cttttaaggc acatgctcat agagcagatg atatgtttac agcaataaga
    44221 attgcaaaag aatttaattt aaagcttaca ttagaccatt gtacagaagg tcatcttatt
    44281 gtagatgaat tagtggaaga agggtttcct gtaatagtag ggccatcttt gtcagaaagg
    44341 tctaaatttg agcttagaaa tttgacattt aatacagcag gaatactttc aaatgcaggt
    44401 cttgatgtat gtataatgac agaccatcca gtaataccag ttcaatatct tccaatatgt
    44461 gcaggtattg cagtaaaaca tggtatgaaa gaagaaaaag caatagaatc aattacgata
    44521 aatccagcta agactttagg aatagaagat agagtaggct ctatagaagt tggtaaagat
    44581 gcagacctag taatttggga taattctcca ctagagattc aaagtaatgt actttacact
    44641 ataataaatg gcaaagtagt gtatgagaaa aaataacttg acaattatta aaaaattaac
    44701 taatataaga gtgtaaggaa aattaaaaaa tcaaagaaga tcttcggggc agggtgaaat
    44761 tccctaccgg cggtatagcc cgcgaactac ttaggtggtt gatttggtga gattccaaag
    44821 ccgacagtaa agtctggatg agagaagata tttatggaag ataaataagt ttattttagt
    44881 ttgtttaaac tatatccata aaaactcgaa gatagggtct tcgagttttt tgtttttcct
    44941 aaaaaatata aacatattga ttagggggaa aataattatg caaaacacag caaaaagagg
    45001 caatatgatt tcaacaagaa ctttagtagt aatgtcaatt ttatctgcaa tatcttattt
    45061 attaatgttt attcaagcac caataccagg tatatttcca gatttcttaa aaattgactt
    45121 atcagatatt ccagcaatat tcggaggaat gtctctagga ccattagttg gatttggaat
    45181 agttgtagta aaaaatgtgt tacaagcaat aacagcaaca acaactggag gaataggaga
    45241 atttgcaaat atattaattg gtggttctta tgtaatgata ctttgttatt catataagag
    45301 aagtggagat ttaaaaggtg tactgattgg ttttgtatta ggaacaatat ctatgactat
    45361 aattggttca ttagtaaatt attttgtaat gttgccactt tatggtcaac ttatgggact
    45421 agatgctata ataggtttag gttcagctat aaatcctcaa gttcatgatt tattcacttt
    45481 tgtaatttgg atgatagctc catttaatgt gctgaaggct gttataatat ctttagtaac
    45541 attgccactt tataaaaaaa tgggaaatat aataaagaaa taattagaca agaaaatata
    45601 aaattaaatg tattgaatag ccataattta tttataactt cagtcctata ttgtgattga
    45661 tatattaaat atattatggc tttattaatt tatcaaaaaa tgtatataaa ataagaaata
    45721 tttagacaaa tttaaatttt aaaataaagg aagaattaaa acgattaact attaaaatat
    45781 gaaaaaggta tctacaatat acagagcgaa tttataataa acaaaaaata aagttattat
    45841 taaaaaacta taaagactaa taatcaaact tttaataata actaatttaa taatgatagg
    45901 ggtgtatatt catgcattta tctaaaaaaa taaaaaaatt atttttagat gataaagggt
    45961 attattttaa tagtaattgg gaagatattc atattggtat ttatgataca aaattaggag
    46021 attctttaaa gtatatcact aatgaatata ttgaaaatat ggaaagggta aaatacgatt
    46081 atcgtgaatt aaaaaatata gaaaatcaaa ctgatggtat gtgtatttta gcaataaggc
    46141 agaattacaa ggcattagag tatgtaaaaa atcaaaccga agaattatgt ttagaggcaa
    46201 taaagtataa ttataaagca cttgaatatg taaaagaaca aacagaatac ttgtgtcttg
    46261 aagctataaa aaaagattgt aatgcactta agtatgtacg aaataaaaca gaagggctta
    46321 ttataaaagc aataagtcat tcttccaata tagatgttgt aagtatacta aaggctttag
    46381 agactcaaac aagaaggata tgtttagaag caattaaaaa agatggcagg tgtcttgcat
    46441 acgttagaga acagagtgaa gaattatgta tagaggccat taagcaaaat tataaagcat
    46501 taaaatatgt aaaaaatcaa acggaaaaaa tgtgtataga atccgtaaga caaaatggta
    46561 tggcattaca atatgtaaat aaacaaactg ataaaatatg tatagaagct gtcaaacaag
    46621 atggaagaag tctacaattt gtcaacaata agacagaaga aatatgtata aatgctataa
    46681 gatatttaaa taaaaaatat aatattaaag atgtactaag ttacatagat aagtatacag
    46741 aagatatatg tatagagatt gtaagacaaa atggaaaaat gcttatgtat ataaaaaatc
    46801 aaacagaaaa aatgtgtatt gaagcagtaa aagagaacta taagtctctt aagtatgtaa
    46861 aagagcaatc tgagagaata tgtaaagaag cattgaagca aaaccataaa gcaaaagagt
    46921 atgtaaaaat tgctattgat gattgtatat aaggtgtaga tacagccatc aatagtgaaa
    46981 gataaaaaaa gataaagaca agtactttat aataatctag gcttatacta taattaatta
    47041 agcagtgata taaattcaca tgacgattat atcaaaatgg agatgaattg atggcaaaaa
    47101 tatatttaga aaatgaaaat aaaactagag aaataggata taaattaggt aaacttttaa
    47161 aagaaggcag tgttatctgt ttagtaggag atttaggagc tggtaaaact acaatgacac
    47221 aaagcttggc agattcatta gggatagaag attatataac aagtcctact tttactataa
    47281 taaatgaata tgaaggaaaa atacctttgt atcattttga tgtttataga ataggtagta
    47341 gtgatgaaat gtatgatatt ggttatgatg aatatgtaaa ttcaaatgga atctgtataa
    47401 tagaatgggc taatctaata gaagatatac ttccaaaaga atatttgaat atagaattga
    47461 gatataaaga tgaaggaaga gaaatgatat taactccaaa aggagagttt tataaagaga
    47521 tagttgagga gttgattaaa taatgaaaat actaggtatg gatacgtctt ctatggcagc
    47581 aagtgtagca gttgttgaag atgataactt aatatgtgag tttacagtaa acaacaaaaa
    47641 aactcattca caaaagctta tgccaatgat agaaaatatg ttgagtatga gtgatttaag
    47701 tataaaagat atggatttat tagctgtatg tataggtcca ggttcgttta caggacttag
    47761 aataggtatg gctacagtaa aagctatggc ccatgtaaat aatataccaa taatagcagt
    47821 aaattctttg gaatctctag caaataacat aaatttctgt gatagaaaga tatgttgtat
    47881 attagatgcg cagaaaaatc aagtttattc agctaaatac aagtttgaaa atggagagat
    47941 gatagaatta gatggtgttg atgttgtaga gtttgatgct ttagtagatg aaattgtatc
    48001 aactaatgaa gaatttataa tagttggaga agctgtatac aaatataaag acaagttaga
    48061 gaatataaaa aatataaaaa taccttcacc agctaataat gtatctaacg caggaagctt
    48121 atgctcttta gcattaaata aatataataa aaatatagat gtacatactt gttatacgat
    48181 aaatcctatg tatatcagaa aatctcaagc tgaagtacaa tatgatgaaa aaatgaagag
    48241 gctaaataat ggaagataat acaaaactaa tagtagatga tataaaaata gaagaaatga
    48301 ctacagaaga tatagatgaa gtttttgaag tagaaaaaaa ttgttttgaa gattattggt
    48361 caaaagagtc ttttagaaaa gagttgtcta atgaagtagc aaggtatata gtagctaagt
    48421 taaatggaaa aattgtcgga tatgtgggaa tttggcttat acttgatgaa gggcatataa
    48481 ataatgtggc tgttcatagt gactatagag ggaaaaaaat aggggacaag ttgataaaat
    48541 gcatagttga tttatgtaaa gataataata ttgcatctat gacattagaa gttagagcat
    48601 caaataaaat agcacaaaat ttatatagaa aatatggatt taaaatgggt ggaataagaa
    48661 aagaatacta cagtgataat aaagaagatg caattataat gtggaaccaa ttaaaagagg
    48721 tgtagagttt atgagtgata taataacatt agctatagag agtagttgtg acgaaacagc
    48781 tgcatcagtt ttaaaaaatg gtagagaagt actttcaaac ataatctcta ctcaaatcga
    48841 gacacataaa aaatttggtg gtgtagttcc agaagtggcc tcaagaaaac atgtggaaaa
    48901 tattgatata gttgttcaag aagctttaga caaagcaaat ataggtttta atgatataga
    48961 ccatatcgca gttacatatg gacctgggct agtaggtgct ttattggttg gtctatctta
    49021 tgctaaagca cttgcatata ctttgaatat tccacttgta ggagtaaatc atatagaagg
    49081 gcatctaagt gcaaactata tagagcataa ggatttaaaa cctcctttta taacattaat
    49141 agtatctgga ggtcatactc acttggttga agtaaaagat tatggcaagt atgaaatttt
    49201 aggaaaaaca agagatgatg catctggaga agcatttgat aagatttcaa gagctatgaa
    49261 tttaggctat ccaggaggtc ctattattga taatctggcc aagaatggaa ataaacatgc
    49321 tatagaattt ccaagagcct atttagaaga agatagctat gattttagtt ttagtggatt
    49381 aaaatcttct gtattaaatt atctaaatgg aaaaagaatg aaaaatgaag aaatagtagt
    49441 tgaagatgta gcagcatctt tccaagaggc tgtagtagag gttttatcta ataaagcact
    49501 aaaagcagta aaagacaaag gatacaatat cataactctt tctggtgggg ttgcttctaa
    49561 ttcggggctt agagctaaaa taacagagct tgctaaagac aatggtatta ctgttaagta
    49621 tcctcctcta attttatgca cagataatgc agctatgata gggtgtgcag gatattataa
    49681 ttttataaat gggaaaacgc atgatatgag tttaaatgca gttccaaatt taaagataaa
    49741 tcagtagagt tagatgaatt tgtaaatata aaagtattgt ttcaaaaaag taagtagaag
    49801 tgaaattaaa tataataaaa ataaatatag tatatattaa aacttgaagt aaaactattt
    49861 ataatcttta tattccaatg aattgcaaca aattttagga tatacagatt gtctttggct
    49921 tagatttgct tcaagtttaa ttttttctat agaaagatta ttttaaaaag tgggtaatat
    49981 accctttttt ttcattctta aaaagtttta tactaattat agaaagatta aggaggggaa
    50041 gaaatgagtc aaagtaaaga agacaaaata agaagtattt tggaagcaaa aaatattaaa
    50101 tcaaattttc aaaataaaga aaatttaagt gaatttaatg aaaaaaaagc aagtaaaaga
    50161 gcagaagact tattagatgt atattataat acattatcta ctgctgatat ggaatttcca
    50221 tattggtata atagagaata tagaaaatca gatggtgata taccagtagt tagaagagct
    50281 aaagctctta aagctgcttt ttcacatatg acaccaaata taatacctgg tgagaaaata
    50341 gtaatgcaaa agacacgcca ttatagaggt tcttttccaa tgccatgggt aagtgaaagt
    50401 ttctttgttg ctcaaggaga acaaatgaga gaagaggcaa agaaattagc cagtaacaca
    50461 gctgatgaac ttacaaaatt tgggtctggt ggaggaaatg ttactgaaag ttttggaaat
    50521 gtcgtttcaa tagctggtaa attcggaatg agaaaagaag aagtacctgt acttgtaaaa
    50581 atggcaaagg aatgggttgg aaagtctgtt gaagatttag gttttcacta tgaaaaaatg
    50641 atgccagatt atgatttaaa agaaaaccta atgagtacac ttatatgcat gtttgattca
    50701 gggtatacac ttcctcaagg tagagaagtt ataaattatt tttatccttt aaattatgga
    50761 ttagatggaa ttatagaaat ggcaaaagaa tgtaaaaagg cagttgcagg aaatgcatct
    50821 ggagatgggc ttattggtat ggaccgtcta tatttttatg aggctgttat tcaagttatt
    50881 gaaggattac aaacttggat tttaaattat gcaaaacatg caaaatattt agaaagtata
    50941 gagacagatt tagaagctaa aaaagaatac tcagatttag tggagatatt agaacatatt
    51001 gcacataaac aacctcgtac atttagggaa gctcttcagt tgacttatac aattcatata
    51061 gcatcagtaa atgaggatgc tatatctggt atgtctatag gacgttttgg tcaaatttta
    51121 tatccttggt atgaacaaga tatagaaaaa ggactaatta caaaagaaga ggttatcgaa
    51181 ttattagagc tttatagaat aaaaatcact tgtattgact gttttgcttc agcgggcgta
    51241 aatggaggag tactatcagg taatacattt aatactttat ctataggtgg attaaaagaa
    51301 gatggttcta caggagctaa tgaactagaa gagttattat tagaagcaag tatgagatgt
    51361 agaactcctc aacctagttt aactatgcta tatgatgaaa agcttccaga agatttctta
    51421 atgaaagctg ctgaatgtac aaagcttggt tctggttatc cagcctgggt aaacaactca
    51481 aatggaacaa catttatgat gaaacaattt gctgatgaag gtatgactgt tgaagaagca
    51541 agagcatttg cattaggtgg atgtttggaa acgtctccag ggtgttggaa acagttaact
    51601 ttaaacggaa aaacgtactc tattgcaggt ggagcaggtc aatctgctgg ctctggagtt
    51661 cactttatag ctaatcccaa aattttagaa cttgttctta tgaatggtaa agaccatcgt
    51721 atgaatatac aagtatttga gcctcataat aagccattag atacttatga agaagtcata
    51781 gaagtattta aagattatta taaacaagca ataaatgttt tagaaagagc aaataacatt
    51841 gagttagata tatggagaaa atttgatact tctataataa attcactact aaagccagac
    51901 tgtttagaca aaggtcaaca tataggaaat atgggatatc gttataatgc aacattaaac
    51961 gtagaaactt gtggtactgt tactatggtc aattcatttg cagcacttaa gaaacttgta
    52021 tatgatgaca aagcatttac aatagaggaa atgaaggatg caatattaaa taattttgga
    52081 tttaaagatg cattagaagt aggaaattat tctatggcag accaagtaaa agtagataag
    52141 actggtaaat atgatgctat ttacaaggcc tgtcttgatg cacctaaata tggaaataat
    52201 gatttgtatg cagataatat tcttaaaaac tatgaggtat ggttatcaaa agtatgtgaa
    52261 gaagcacaat ctttatatgc taagaagatg tacccttgcc aaatatctgt ttctacacat
    52321 ggaccacagg gagctgctac cttggctact ccagatggta gactaagtgg aacaacatat
    52381 tcagatggtt cagtctctgc atatgcagga acagataaaa atggagtata tgcattattt
    52441 gaatctgcaa ctatttggga ccaagcagta gttcaaaatt ctcagatgaa tttaaaactt
    52501 catccaacta ctataaaagg acaacaagga actaaaaaat tattagattt aacaagaagt
    52561 tatttaagaa aaggtgggtt ccatattcaa tataatgttg tagattcaga aacattaaaa
    52621 gatgcacaaa aaaatcctga caattatcgt caattaatgg tacgtgttgc tgggtttact
    52681 caatattggt gtgaattagg taaaccaata caagatgaag taattgctag aacagagtat
    52741 gaaggggtgt aaaatatgag aaagcatagt gattgtatga atttttgtgc agtagatgca
    52801 accaaaggaa tttgtagatt atcaaaacaa atgattaatt tagatgattc agcatgtcca
    52861 gagataaaag taatgccaaa atgtaaaaat tgtaaaaatt ttgttgaagc taatgatgaa
    52921 ggtataggca aatgtgttgg cctagagaaa gaagactggg tatattcaac attgaatgca
    52981 attacttgtg aaggacatgt gtttaatgag tagtcaaaag caattagaag gcatgatttt
    53041 cgatgtacaa agcttttcag ttcatgatgg tccaggttgt agaacaactg tatttttaaa
    53101 tggatgtcca ctgagttgta aatggtgtgc aaatccagaa agttggactg taagaccaca
    53161 tatgatgttt agtgaattat cttgtcaata tgaaaatgga tgtactgtat gtcatggtaa
    53221 gtgtaaaaat ggtgcattga gctttaatct tgataataag ccagttatag attggaacat
    53281 ctgtaaggac tgtgaaagtt ttgaatgcgt caactcatgt tattataatg cgtttaaatt
    53341 atgtgcaaaa ccttatactg tagatgaact tgtgcaggta attaaacgtg attctaacaa
    53401 ttggagaagt aatggaggag taacatttag tggaggtgag ccactacttc agcatgaatt
    53461 tttacatgaa gtattattaa agtgtcatga ggtaaatata cacactgcca ttgaaacaag
    53521 tgcatgtgta tcaaatgaag tttttaataa aatatttaaa gatattgatt ttgcatttat
    53581 tgatataaag catatggata gagaaaaaca caaagaacaa acaggtgtat ataatgattt
    53641 aatattagaa aatatatcaa accttgcaaa ttctgactgg aatggaaggt tagtacttag
    53701 agtaccagtt atctctgggt ttaatgatag tgctgaaaat ataagtgata ttatatcttt
    53761 tatgcacaaa aataatttaa tagagattaa cttattacca tttcataggc taggagaatc
    53821 aaagtggatt caattaggta aggagtatga gtattctgat aagggagata ttgatgaaga
    53881 gcatcttgaa gaattacaag atatattttt ggataatggt atagcttgct atgttggtca
    53941 tgagacagct ttttaaaatc aaatacaagt ttttttaatt aaaaaaggat gttacactaa
    54001 aagctagagt tacatccttt tttatgtggt ttttatgtag aaataattaa gtaaagtttt
    54061 atatgtttta tatattgttc atattgcttt atgtccataa atattacttt aaatttatac
    54121 tttatatatt ttttatagtt taaattacag ttagagaata tattacgttt actttattga
    54181 agcagaagtt ttgtggtaaa atactactat gaagaaattt aatagaatat attttagaaa
    54241 caactaaaaa taatagttag gaggaagaca tctatatgaa caaatcatgg agtaacttga
    54301 ttattaagaa taaagacaaa aaagtacgtt cagggtggat tattataact gttatggctg
    54361 ccttttacat atttgagtat tatttaagca ctatacttat ggaaataata agaaaaattc
    54421 tgattgctac aggtgacatt aatttaaaaa caaactattt ttcggaatat gctaattggt
    54481 taaatgacgt tgggttacag gttgtatttc aaattctaac agattcactt atgattatta
    54541 ttcctataat tgtatggaga tttattatga aaaatcctat tagtgaaatg ggattacatt
    54601 cttatagaat taagaaaaaa gaaggatatg taggaatgtt attaggaata tttaattgta
    54661 cagtaatctt tattataaca ataacaattg gtggtggtca tgttacttca tgggtaccaa
    54721 aaatttcacc tttaacagtt tcttggatac ttgtctttat tttagttgct tttgcagaag
    54781 aaattttaaa tcgtggtttt tttatggcag ttttacgaag atgtaaaaat atttatttta
    54841 taatgattgt tccatctgtt atttttgggt tgattcatat atggaatcct gatgttactt
    54901 ttttgtctgt aattaatatt ataattattg ggattctttt ttcatatatg tttataaagt
    54961 cgtcgaatat atggatgtgt ataggttatc attttacatg gaatgtattt caggggatta
    55021 tatatggtat gcctgtaagt ggacttcagg ttccagggat tataactaca caaatcactc
    55081 atggtaatct attaaatgga gggatgtttg gtattgaagg tggaattttg actacttttg
    55141 ttaatctact tagctttata tttgtatggt attactatag agattctaag tatgattttg
    55201 ttagtgataa agtaaatgtg actaatatca agaattagtt gattataata aattttaata
    55261 atatcttcta aacttaaagt attctgtagg attgttggaa atttacaaaa atataaaaat
    55321 aacttggata ttctatttac tccaagttat ttttatattc ttttgtaaat agaaatggaa
    55381 cataaaaaat taaaacaacc atagatacta ttagtatatc atggtaagtg atagttgatt
    55441 ctttagaatt tgtgataaga aaagcactat tatttaaaac atgaattata gcacaaaacc
    55501 agatattctt agtttttata taaagatatc ctaaaaatat tgaaattcca actatatgta
    55561 ttgaacgtaa tattactcct aatattggcg cttcaggtgt atataatgta aatattagtg
    55621 gtaagtgcca caagctccaa cacataccta atattatgac tcctaatttt ttaccaaatt
    55681 tatcaaaaaa aatattctgt aaaaaacctc tccaggcata ttcttcacta aaaaaaatat
    55741 aaatagtagt taatgatgtt attggagtaa atataaattt tatgatacca ctataattag
    55801 gttgagcccc ttgtagtatt agtataaatg atgttggaac aattacagaa atcataaagt
    55861 aaataattaa taaagaacct agtttaatat tttttacagg atataaattt tttttatttg
    55921 atgaatatat tataattgat aagctactta taaaagttaa tattaggttt ataattataa
    55981 attcattatt atttattaga tttaataact taagaac
//
LOCUS       DAELXX010000012        52598 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.012, whole genome shotgun sequence.
ACCESSION   DAELXX010000012 DAELXX010000000
VERSION     DAELXX010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 52598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52598
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.012"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(40..2373)
                     /locus_tag="KPH29_000718"
     CDS             complement(40..2373)
                     /locus_tag="KPH29_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG1466348.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQAGAKGGA
                     KNPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLD
                     TTKNDASKENSDENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYG
                     VNQKVINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYGY
                     GNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLL
                     RIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKTVINQIRTINLK
                     EKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGD
                     VYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(2348..3064)
                     /locus_tag="KPH29_000719"
     CDS             complement(2348..3064)
                     /locus_tag="KPH29_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1466349.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMRLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVNIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAINTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
     gene            complement(3479..4135)
                     /locus_tag="KPH29_000720"
     CDS             complement(3479..4135)
                     /locus_tag="KPH29_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG1466350.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGASIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
     gene            complement(4190..4819)
                     /locus_tag="KPH29_000721"
     CDS             complement(4190..4819)
                     /locus_tag="KPH29_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBG1466351.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSKHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            complement(4950..5591)
                     /locus_tag="KPH29_000722"
     CDS             complement(4950..5591)
                     /locus_tag="KPH29_000722"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBG1466352.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTVSVTAI
                     KGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            complement(5600..6271)
                     /locus_tag="KPH29_000723"
     CDS             complement(5600..6271)
                     /locus_tag="KPH29_000723"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBG1466353.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLPDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            complement(6276..7160)
                     /gene="rsgA"
                     /locus_tag="KPH29_000724"
     CDS             complement(6276..7160)
                     /gene="rsgA"
                     /locus_tag="KPH29_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBG1466354.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDEDEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            complement(7258..7995)
                     /locus_tag="KPH29_000725"
     CDS             complement(7258..7995)
                     /locus_tag="KPH29_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBG1466355.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKADISNREDVENMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            complement(8103..10118)
                     /gene="pknB"
                     /locus_tag="KPH29_000726"
     CDS             complement(8103..10118)
                     /gene="pknB"
                     /locus_tag="KPH29_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBG1466356.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     EEEEEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGSSNSGNSGGNSGNNGGNSGGSNSGNSGGSSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            complement(10135..10887)
                     /locus_tag="KPH29_000727"
     CDS             complement(10135..10887)
                     /locus_tag="KPH29_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBG1466357.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            complement(10907..11938)
                     /gene="rlmN"
                     /locus_tag="KPH29_000728"
     CDS             complement(10907..11938)
                     /gene="rlmN"
                     /locus_tag="KPH29_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBG1466358.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGNIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            complement(11946..13271)
                     /gene="rsmB"
                     /locus_tag="KPH29_000729"
     CDS             complement(11946..13271)
                     /gene="rsmB"
                     /locus_tag="KPH29_000729"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBG1466359.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            complement(13333..14034)
                     /locus_tag="KPH29_000730"
     CDS             complement(13333..14034)
                     /locus_tag="KPH29_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBG1466360.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            complement(14103..14855)
                     /locus_tag="KPH29_000731"
     CDS             complement(14103..14855)
                     /locus_tag="KPH29_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HBG1466361.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTAVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNVGELI
                     KLKEKTNVKIFVATGGTLARKIIMDNKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDMMDIEKAIDFLTGKNILICN"
     gene            complement(14865..15794)
                     /locus_tag="KPH29_000732"
     CDS             complement(14865..15794)
                     /locus_tag="KPH29_000732"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBG1466362.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTIYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            complement(15809..16249)
                     /gene="def"
                     /locus_tag="KPH29_000733"
     CDS             complement(15809..16249)
                     /gene="def"
                     /locus_tag="KPH29_000733"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBG1466363.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            complement(16267..18792)
                     /gene="priA"
                     /locus_tag="KPH29_000734"
     CDS             complement(16267..18792)
                     /gene="priA"
                     /locus_tag="KPH29_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBG1466364.1"
                     /translation="MNNILNLSRGFIMKKYAKVIVRSNTIYTDNLFTYQIPVFLSDVI
                     KIGHRILVPFGKGNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPIFRKEDLELVYWM
                     KNRYLCTYIECINLIYPKGYKLNNYKVVLLGESLSGLNEIELKEKISTLSDKNREIVE
                     KVIDSKGKIKVDKLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESD
                     KIDDYIEQNKINVGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITLE
                     FKDYYREPKSIYKSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVY
                     MEIIEFALNQGLDSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAV
                     KAGKVRVLIGARSALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNI
                     TLILGSATPSIEEYYRAKSGEYKLINIKSRANDKPLPNIEVVDMKDELDKGNRSIFSL
                     KLQKEIRYAIEENNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRC
                     HYCGYEKEIPKECPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGAL
                     DEILNKFKDKEADVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLIT
                     QVSGRAGRADKEGKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNML
                     SVVVSGEDERLVIKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQIL
                     FKDENIEINLLKGIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            complement(19031..20230)
                     /gene="coaBC"
                     /locus_tag="KPH29_000735"
     CDS             complement(19031..20230)
                     /gene="coaBC"
                     /locus_tag="KPH29_000735"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBG1466365.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLAFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            complement(20235..20501)
                     /locus_tag="KPH29_000736"
     CDS             complement(20235..20501)
                     /locus_tag="KPH29_000736"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBG1466366.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            complement(20504..21121)
                     /gene="gmk"
                     /locus_tag="KPH29_000737"
     CDS             complement(20504..21121)
                     /gene="gmk"
                     /locus_tag="KPH29_000737"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBG1466367.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLEELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            complement(21151..22032)
                     /locus_tag="KPH29_000738"
     CDS             complement(21151..22032)
                     /locus_tag="KPH29_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBG1466368.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            complement(22036..22866)
                     /locus_tag="KPH29_000739"
     CDS             complement(22036..22866)
                     /locus_tag="KPH29_000739"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBG1466369.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKINLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGVLEV
                     "
     gene            complement(23153..24187)
                     /gene="ltaE"
                     /locus_tag="KPH29_000740"
     CDS             complement(23153..24187)
                     /gene="ltaE"
                     /locus_tag="KPH29_000740"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBG1466370.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGSKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            24483..26258
                     /locus_tag="KPH29_000741"
     CDS             24483..26258
                     /locus_tag="KPH29_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HBG1466371.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDSLHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEIANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIVLSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            complement(26473..28125)
                     /locus_tag="KPH29_000742"
     CDS             complement(26473..28125)
                     /locus_tag="KPH29_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG1466372.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCSHSKEKSLLNAPDKFEEYLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGLLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     IQDGMEIISKNLLEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGCFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYIISRNKKTPSRDKQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQITKLKNSF"
     gene            complement(28424..29635)
                     /locus_tag="KPH29_000743"
     CDS             complement(28424..29635)
                     /locus_tag="KPH29_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBG1466373.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKIVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLLLRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISIGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            complement(29919..30452)
                     /gene="pyrR"
                     /locus_tag="KPH29_000744"
     CDS             complement(29919..30452)
                     /gene="pyrR"
                     /locus_tag="KPH29_000744"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBG1466374.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            complement(30811..31758)
                     /locus_tag="KPH29_000745"
     CDS             complement(30811..31758)
                     /locus_tag="KPH29_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBG1466375.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKAKYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDFVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEAIKKIK"
     gene            complement(31727..32173)
                     /gene="lspA"
                     /locus_tag="KPH29_000746"
     CDS             complement(31727..32173)
                     /gene="lspA"
                     /locus_tag="KPH29_000746"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBG1466376.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLIDR
                     VRLGFVVDYFDFRVIWEYVFNVADVFVVVGTVFLCIYVLFFESKSR"
     gene            complement(32483..33262)
                     /locus_tag="KPH29_000747"
     CDS             complement(32483..33262)
                     /locus_tag="KPH29_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG1466377.1"
                     /translation="MKKAIWGICICLLSIVGVYTYNHNTHNQNSSKETYTLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQILDILKKNNVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYGSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDTIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            complement(33312..33794)
                     /locus_tag="KPH29_000748"
     CDS             complement(33312..33794)
                     /locus_tag="KPH29_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBG1466378.1"
                     /translation="MDTKKYKDKLLKEREKLTNLVVDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDSALSKIENGTYGFCDLCHNQI
                     DLERLDILPDTNLCNNCAKKENALLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGTLRD
                     "
     gene            complement(33978..35399)
                     /locus_tag="KPH29_000749"
     CDS             complement(33978..35399)
                     /locus_tag="KPH29_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBG1466379.1"
                     /translation="MVSFLGSIVVLIVGYFIYGTFVEKVFGINDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGIVVAI
                     ILGLLFFKKAKDIKEQNAHKLAS"
     gene            complement(35545..36315)
                     /locus_tag="KPH29_000750"
     CDS             complement(35545..36315)
                     /locus_tag="KPH29_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1466380.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSSIEVVNEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYENYAVDAFSLDVFDYVLKP
                     YSEERIISMLNKLEKSEMSDIELSNSNSNLYKYKKETVNQEIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGISEVENLINDKTFFRTHRSYIVNLTKV
                     KEIIPWFNNTYILKLKNSDYEVTVSRSKVKEFRLLMHI"
     gene            complement(36320..37522)
                     /locus_tag="KPH29_000751"
     CDS             complement(36320..37522)
                     /locus_tag="KPH29_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG1466381.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIVGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRTDNGT
                     LIVFYVKE"
     gene            38394..39140
                     /gene="cdtR"
                     /locus_tag="KPH29_000752"
     CDS             38394..39140
                     /gene="cdtR"
                     /locus_tag="KPH29_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family response regulator CdtR"
                     /protein_id="HBG1466382.1"
                     /translation="MDILIFDNDVCFGIKLKEKINNILIKEGFDNDVIRLYYNANLLL
                     KELTEKNKVKIYFIVVDAKYKISNELCDGLWIAQKIRESDYISPIIFLTNHIEMILGI
                     FDYRLEVMDFILKHDMEIAESKIKACIKIAHKRYVKEKNYRSNFFTIYSDSSLWKISF
                     DEVIYFETSAIPHKIKLVTTSRIFEFYKSLRSLSDLDACFIRVHKSFVVNKYHIVSLD
                     LKKNNIKMSNGHICRISNTYRNILKNIIKT"
     gene            39530..40921
                     /gene="cdtA"
                     /locus_tag="KPH29_000753"
     CDS             39530..40921
                     /gene="cdtA"
                     /locus_tag="KPH29_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylating binary toxin enzymatic subunit
                     CdtA"
                     /protein_id="HBG1466383.1"
                     /translation="MKKFRKHKRISNCISILLILYLTLGGLLPNNIYAQDLQSYSEKV
                     CNTTYKAPIERPEDFLKDKEKAKEWERKEAERIEQKLERSEKEALESYKKDSVEISKY
                     SQTRNYFYDYQIEANSREKEYKELRNAISKNKIDKPMYVYYFESPEKFAFNKVIRTEN
                     QNEISLEKFNEFKETIQNKLFKQDGFKDISLYEPGKGDEKPTPLLMHLKLPRNTGMLP
                     YTNTNNVSTLIEQGYSIKIDKIVRIVIDGKHYIKAEASVVSSLDFKDDVSKGDSWGKA
                     NYNDWSNKLTPNELADVNDYMRGGYTAINNYLISNGPVNNPNPELDSKITNIENALKR
                     EPIPTNLTVYRRSGPQEFGLTLTSPEYDFNKLENIDAFKSKWEGQALSYPNFISTSIG
                     SVNMSAFAKRKIVLRITIPKGSPGAYLSAIPGYAGEYEVLLNHGSKFKINKIDSYKDG
                     TITKLIVDATLIP"
     gene            40974..43604
                     /gene="cdtB"
                     /locus_tag="KPH29_000754"
     CDS             40974..43604
                     /gene="cdtB"
                     /locus_tag="KPH29_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094607992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylating binary toxin binding subunit
                     CdtB"
                     /protein_id="HBG1466384.1"
                     /translation="MKIQMRNKKVLSFLTLTAIVSQALVYPVYAQTSTSNHSNKKKEI
                     VNEDILPNNGLMGYYFTDEHFKDLKLMAPIKDGNLKFEEKKVDKLLDKDKSDVKSIRW
                     TGRIIPSKDGEYTLSTDRDDVLMQVNTESTISNTLKVNMKKGKEYKVRIELQDKNLGS
                     IDNLSSPNLYWELDGMKKIIPEENLFLRDYSNIEKDDPFIPNNNFFDPKLMSDWEDED
                     LDTDNDNIPDSYERNGYTIKDLIAVKWEDSFAEQGYKKYVSNYLESNTAGDPYTDYEK
                     ASGSFDKAIKTEARDPLVAAYPIVGVGMEKLIISTNEHASTDQGKTVSRATTNSKTES
                     NTAGVSVNVGYQNGFTANVTTNYSHTTDNSTAVQDSNGESWNTGLSINKGESAYINAN
                     VRYYNTGTAPMYKVTPTTNLVLDGDTLSTIKAQENQIGNNLSPGDTYPKKGLSPLALN
                     TMDQFSSRLIPINYDQLKKLDAGKQIKLETTQVSGNFGTKNSSGQIVTEGNSWSDYIS
                     QIDSISASIILDTENESYERRVTAKNLQDPEDKTPELTIGEAIEKAFGATKKDGLLYF
                     NDIPIDESCVELIFDDNTANKIKDSLKTLSDKKIYNVKLERGMNILIKTPTYFTNFDD
                     YNNYPSTWSNVNTTNQDGLQGSANKLNGETKIKIPMSELKPYKRYVFSGYSKDPLTSN
                     SIIVKIKAKEEKTDYLVPEQGYTKFSYEFETTEKDSSNIEITLIGSGTTYLDNLSITE
                     LNSTPEILDEPEVKIPTDQEIMDAHKIYFADLNFNPSTGNTYINGMYFAPTQTNKEAL
                     DYIQKYRVEATLQYSGFKDIGTKDKEMRNYLGDPNQPKTNYVNLRSYFTGGENIMTYK
                     KLRIYAITPDDRELLVLSVD"
     gene            complement(44256..45257)
                     /gene="trpS"
                     /locus_tag="KPH29_000755"
     CDS             complement(44256..45257)
                     /gene="trpS"
                     /locus_tag="KPH29_000755"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBG1466385.1"
                     /translation="MSEKKVIFSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANAIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
     gene            complement(45280..45978)
                     /locus_tag="KPH29_000756"
     CDS             complement(45280..45978)
                     /locus_tag="KPH29_000756"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBG1466386.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILKGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     misc_binding    complement(46021..46261)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(46352..47755)
                     /locus_tag="KPH29_000757"
     CDS             complement(46352..47755)
                     /locus_tag="KPH29_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG1466387.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFMLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     TSLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
     gene            48252..49328
                     /locus_tag="KPH29_000758"
     CDS             48252..49328
                     /locus_tag="KPH29_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG1466388.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
     gene            complement(49431..50273)
                     /locus_tag="KPH29_000759"
     CDS             complement(49431..50273)
                     /locus_tag="KPH29_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBG1466389.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEIGSVMGTYTSKGAIL
                     ITAI"
     gene            50457..51029
                     /locus_tag="KPH29_000760"
     CDS             50457..51029
                     /locus_tag="KPH29_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG1466390.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            complement(51192..51464)
                     /locus_tag="KPH29_000761"
     CDS             complement(51192..51464)
                     /locus_tag="KPH29_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466391.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDGHTHEHK"
     gene            complement(51591..52397)
                     /locus_tag="KPH29_000762"
     CDS             complement(51591..52397)
                     /locus_tag="KPH29_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG1466392.1"
                     /translation="MGMILSTLGINLYLAPAKLLSGGVAGICVILYKLFGINQGISSF
                     LMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLTQDIAKYFPIDDTMLQCIYGGAL
                     TGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNTFLFINFFVVCAGAFLFGAKLVM
                     YTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEISKVIMNKMGRGVTFLEAEGAYT
                     QNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNNVEEVKGGGFKDKFL"
BASE COUNT        17542 a         9073 c         5671 g        20312 t
ORIGIN      
        1 tttattctat agactttata attattaaag aagtctaatc taatttttgt taaaagtaat
       61 tgtaacttta aataaatcac catccacact tatattcatc ttaccaccct gtaattctat
      121 aaaacttttt gctattgcta aaccaagacc agaaccttct gtgttacgtg atttatctcc
      181 tcttacaaat ctctccatta tatcatctac actaaaatca atctcagatg cagacatatt
      241 tttaagcata atatttactt tattatctgt ttctaaaata tcaatataca ctcttgaatt
      301 tggcattgca tatttgctta tatttattat aagattttca aatactctaa atgtacgctg
      361 actatctaat ggtaatatta ttttttcatt agaaaagtta ttctttacta ttagagatga
      421 atctgcaagt ttgtcatcta attctaaaag tgtctgcttc attagagata caatatccac
      481 atttactata ttcaaataaa catctccact atttgccttg cttacttcaa acaaatcttc
      541 tattagatgt tgcaatcttt gtgactttct gtctaatgta tcaatataca acttacgatt
      601 ttcatctgtt atactttcat cttttaataa atctatataa gtgattatag atgttagagg
      661 tgtttttaag tcatgtgata catttgatat aagctctgtt ttcatctttt gactcttaac
      721 ctcttcgtca acagcttttt taaatccagt ttgaatattt cctaattcgt cttttatagg
      781 attaaaaaat cctaaatctt tttccatatt tacatctaag tttccatctg aaattttctt
      841 tgtaacatct aaaagttgat tatattcata tattgtcttt ttagagtaat ctgacagtat
      901 cttaaataat actactgaat atattaatgc gagtattatt ccaaagaacc acatactaca
      961 cattataact ataatcaata aatttattcc caaaattgta actatagttt tatttggctt
     1021 ttcttttaga tttattgttc taatttgatt aataactgtt tttcctgctt tttttaacca
     1081 tacaaaaaac attcctgtca cagtatgttt tgtaaaatac tctcttattc caacatttaa
     1141 aatatgtttt aaaagtatta taaatacaaa tattattgag aatattacaa accaataagt
     1201 taaattcatt aaacttacca atatgctagc cattttagca cttatgtcat tgtttataag
     1261 gtactctaca taattcccac tcaaggtatt acttattata acagtaggat ataaatctac
     1321 aagcccctgt ataatagcaa gagcaataaa gagtatctct aacggtattc ttaataatgt
     1381 atcaaatcct agcaactctt tagattttct atatgggaat gctaaagcta caataacaac
     1441 tatagctaat ataattttta aaatcattcc agaaatctta taataagaat tattttctac
     1501 attgcttata tatgaaatta taccatcatc aaaataagta tttccataac catactctac
     1561 actatcacct ttacttatta ctttaggtac agcatatact aatgttatat tttttattgg
     1621 agataactta tatgaaattg aatttcctcc taaacttgta tccattaaat cttctcttgc
     1681 tttagataac aacatttgat taataacttt ttgatttact ccataagaat ccacaatatt
     1741 tacttttcca tctttatcaa aattcattac aacgtagaat ctatagtttt cctttatatc
     1801 tgtaatctgc ttttgtcttt cctcatcatt catttctgta ttttttgact gattttcatc
     1861 agaattttct tttgaagcat cattctttgt tgtatcaagt actcctaatt ctttattatc
     1921 attaaaaaat tcaaaattgc tttctttatc aactaaatag tactttaaat tctttcctga
     1981 cgctttaatg aaatgctccc aattttcaaa gttttcatta aaatctgcat taatactatc
     2041 atctgtataa taagtttctg aaccatcata agtatctttt tctttactta cattttttat
     2101 atagttcata gtttcttcat tgataaatag ctttgatggt tgtatcatct taccttcttg
     2161 ttcttccttc atattatgat ataaaacata acttccttta tatatactat ctacaaagct
     2221 ttcctgttca aatggattct ttgctccacc tttagcacct gcttgtatag tagaatattg
     2281 actaatcata cctaatgatg ctgataacat aagtataaaa actataatag taactatata
     2341 tttatcttta aattttttca attttgtatc caacacccca caccaccttt aaatatttag
     2401 gattttttgg gtcaatttct atcttttccc ttatattcct cacatgtacc ataactgtat
     2461 ctgtatttat agcattttca ttccaaactc tttcatatat ttcatcagca gaaaaaactc
     2521 ttcctgcatt tctcataagt aaacttagta ttttaaattc tataggagtt gtttttatat
     2581 attttccatc aatattaact tcttttgtat tttcatttaa ttctaatccg cctattgcaa
     2641 acacactatc atcactttct accttttgct ctttatttcc aaccatgttt agatacttag
     2701 tgtaccttct aagttgtgaa ttaactctag ccaatagttc taatggctta aaaggttttg
     2761 ttacataatc gtctgctcct atattaagcc ccattatttt gtcaacttct tctgattttg
     2821 cagatagcat tattactgga aaattatagt tttctctaag cctcattgta agtgttatac
     2881 catccatttt aggcatcatt atatcaacta tagctaaatg tatttcttgg ttttctataa
     2941 ccttaagacc ttctaatcca ttgtatccct taaaaacatt atatccttga tttaatagat
     3001 atatctctat agcatctgcg atctcctttt catcttctac caccaaaata ttatatgaat
     3061 tcattttaat ctcctttata ttaatttata aattttcttt acatatatga ttatacaata
     3121 tgatagaagt ttttccattt atatatatat ttatatcttt tgttctggtt atttgtattt
     3181 aatgtccatc ttagaaacct ccttctagtt taaacttaat attttttcat tttgttttat
     3241 atacttaata ttaaaattaa aaacttaaga tattacatat acttttctaa agattttcta
     3301 aagatttaaa tatatttaga attaatatat aaaaaaaagt agttcttatt gtattaatta
     3361 aatcaatgat ttgaaatata aaattaatat caataaaacc tactttttaa ttttttaaat
     3421 atttattttt aatttatctt gtttttaatt tactttgatt ttaaaatgtc cttgactttt
     3481 attttataaa tgtacatatt tcttctactg gttttttaga tggactcttc aaattgtcct
     3541 ctggcactcc aagagctaac attgcaccta aaaaatactc tcccttttca aaaccagttt
     3601 ctgcttctaa aacagcttct atctcatctg ctgcatagtt ttgacttgtc aaccaacaag
     3661 aaccatatcc aagttcaatt gctgatagtg taaagttttc aattgaagca cctagactct
     3721 gcatacctgg atttcttata aataatttat ctatagtctc cttaggtgca tctataagct
     3781 ctaattcata atatcctgat ggattatata cctttgtaaa aactaatact aaaactggag
     3841 cttttaagta aaatagtgta aagtttttaa caaattttct aaatctatta gccttatcca
     3901 ctgatacttt atccatttcc acaagtattt cttgttgctt tttagttatc acatctgcta
     3961 tcttttccat taaatcacgg cgttttataa caacaaaatg ccagttttga atattcttgc
     4021 ctgatggtgc tgcacctgct gcttttatca tttttaaaat atcttcatct gatacatctt
     4081 gatttttaaa ctttcttaca ctttgtcttt taaaaatagt atcttgtaat tccattttta
     4141 aatcccccca taactttttt attattttaa tataattttt aaactcatct tattttaata
     4201 tatcatgttc taacaaatct ttaaatgaat ctataaaata atcatataca tctacattcc
     4261 caaacccata tttggcatat ataaaaggta tacctacaaa cttagcacta tttgcatcac
     4321 cttgcgtatc accaacatag attggattta ttaatttatt tctttcaatt ataagtttat
     4381 tattttctcc tttatgcaaa ccagtattac caggacattc aaaatctata aaatactgtt
     4441 ctagtttatg tgcttttaaa aaagattcta tatatccagc ttgacaatta cttactataa
     4501 ataatttata ttttttagat agtaatttaa gagtatcttc tatatcatca aataatttag
     4561 ctccattttc agataaatag tcacattctt tcaaagaaca ttcattcaat aaattcattc
     4621 tttcttcttc acttaaattt ggaaataaac gatttgctat ttcctcaaaa gaaagcccca
     4681 taagacttct aaataaatct tttgtgacac ttaattctat ttccttatgt ttacttaaaa
     4741 catcctgcca gactttgcat acttcttcag ttgaatccca caatgttcca tctaaatcaa
     4801 aaataatact atctaccaca aaaaatcccc cttatgtcaa agtttattat atatttataa
     4861 ttatatacta aaaagcagat acaatcataa atatatttta cttgattgta tctgctctac
     4921 aatataaaac caattgtcaa aatcaaattt tataaatttc taactacaag tacacagcct
     4981 tgctctactt taatcttaca agaattactc aacataacat ttgatattcc aataggtaca
     5041 gagtatttca tataataatt atcgagaggg tattctaagc cagtaagtgt tacaccctta
     5101 gcatcacctt ttatagctgt tacagaaaca gtgtcccctg ggtttccata caatgaaatt
     5161 tcctcatttt caactatata catctcttct ttatcagaaa gtatcctaga atatatacca
     5221 tcttctttta aataatataa tagctttata tttgcaagag tgtggtcaat ccttccccct
     5281 aaagcaccaa aaaaatcaat atgatttgct ttaagtgtct tagctaaaaa aagacaaagc
     5341 tctgtatcag tctcatcttt tttagatgga aatttttcaa atttaacacc tttattttta
     5401 tagaagttta tcttttcttc ttctactgaa tctaaatctc ctaatatata atctggcatt
     5461 atttccattt tataagtgtg atttgcccct ccatctgcac atattatgca gtcataacat
     5521 tccttaatta taatatctct tgtaacttcg taatcttcaa tttcgccatt taaaacaata
     5581 cagattttca ttattaaatt taaactaatt cttcaagaga agcattcttt ctaaataaat
     5641 taactgtttc ttgaatgtca tcacttccaa atattgcaga acctgctact attacattag
     5701 ctcctgcttg tacaacttcg gctacgtttt tagggcttat accaccatca acttctatat
     5761 caatgtttaa tccttgacta tctataatag cttttaactc tttaatctta tctaaaacac
     5821 aaggaataaa tgattgtcct ccaaatccag ggttaacaga cattaataat accatatcta
     5881 aatcaggtag tacatgttta atcgtatcta ctggtgtagc tggattaagt actacaccag
     5941 cttttattcc atgtgatttt atattttgta tagttctatg tagatgaaca caagcctcct
     6001 ggtgaacagt tattatatca catcctgctt ttacaaattc ttcaatatac tggtctggat
     6061 tttctatcat aagatgagca tcaaatacca tatttatatt ttctttcttt aaacttttga
     6121 ctacaagtgg tcctaatgta atgtttggta caaagtgtcc atccattaca tcgatatgaa
     6181 gatattcaca tcctgcactt tctacttttc ttacatcttc taataatttt gcaaaatctg
     6241 ctgataatat tgatggtgct aatttaatca tttttctaat acctcctact attattttgt
     6301 cttatttcat gtagcagctg tatataacta tcatatcttt ctttagatat ttctccattt
     6361 gtaacagctt cttttatagc acaattaggt tcattttcat gaatacactt tgaaccaaac
     6421 ttacaatcat taaatttatc aaattctata aaataatctt taagttcaac ttcctctata
     6481 tcttcaagtg caagggaact aaaacctggt gtatctgcaa ccattccacc aaattccaat
     6541 tttaaaagtt cagcgtgacg agtagtatgt tttccccttt ttattttatc acttaccact
     6601 cctgtttgaa gtttaaaatt ttcatctatt tcatttaata agcttgattt tccaactcca
     6661 gatggtcctg caaacactac tacattttct tttaattctt cttttacttt atctatattt
     6721 aacttagtaa tattgcttac aggaatgact ttatagccac taagttcata tatattttta
     6781 acagtttcta atgtatcatt atcatctaaa tctgcttttg taaggattat tacagtttct
     6841 aaattttctt tttctgccaa aactataaat ctatctaaaa gagataagtt tggtttagga
     6901 ttttttattg cgaatactat taatgcttta tctacatttg caataggagg tcttataagt
     6961 tctgtatctc tactgtcgat ttcttctaag ataccctttt tttcatcttc atcaacaatg
     7021 cttattttga ctctatcacc aaccaaaggt gttattttct gctttctaaa tatccctcta
     7081 gccttacatt cataaatacc attataagta tctacataat aaaatccact aatccccttt
     7141 ataatttttc cttctagcat caaatgcctc cctttataaa atttatataa acacatactg
     7201 tttatgaata gtatatcgtg ttattctttc ctatttttat ataataccac atttttttta
     7261 aataacaaat cctccgtttg gactaattac ctgaccagtg ataaaatttg atttgtctga
     7321 tgccaagaaa atagcacaat ttgctatatc ttctggagtt cctagcctca ttagaggggt
     7381 ttcttccaca agagcgtcta tatcttcctc attatatcca gataacatgt cagtattaat
     7441 gacacctgga gctatgctat ttactctaat atttgatggt cctacttctt tagctaatgc
     7501 ctttgtcata cctattatac ctgcttttgt tatagaataa tgcacttcac aagacgcacc
     7561 tgatataccc cagattgaag atatattaat tatatttcct tttttttctg atatcatgta
     7621 ttttaaagca acttgcgaac aataaaagct tccttttaaa ttaatattca tcatattatc
     7681 ccaatcttca tcggttatat ctgtaaacaa cttatcttga cttacaccag cattgtttac
     7741 aagtacatcc aatcctccaa attcttttat acaataatct accatattct ctacatcttc
     7801 tctatttgaa atatcagcct taaaaagttt tacagaaaaa tttttttcat ttaatatagt
     7861 atacaactct ttagcttcat tttctgactt attaaaattt actaatacat tgtaaccttc
     7921 ttttgcaaaa gctttagaca tagctttacc aatccctcta gctcctcctg taattaaaac
     7981 ggttttcttc ttcataaata cttttctcct tatattataa ccaattcagt attttataca
     8041 aaacaaactg ccctttaaag aaaatatttt ctataaaagg acagctctta ttttattaat
     8101 atttattctg ttttaccaat gtttgcgcca gttcctgatg aagttcctcc attatctcct
     8161 gagtttgagc ctcctgaact atctccattg tctcctgagt ttgaacctcc tgaacttcct
     8221 ccattatctc ctgagtttga acctcctgaa cttcctccat tatctcctga gtttgagcct
     8281 cctgagcttc ctccactatt tcctgagttt gaacctcctg agttacctcc attattgcct
     8341 gagtttcctc cactatttcc tgagtttgaa cttcctgaat ttccaccact gttccctgag
     8401 tttgaaccag gttctgttgg tgtattattc tctggtgttg ttggtttttt atctggtact
     8461 gttggagtct gtgtatttga tttttctgaa cctttgctta catataggtt tacctcatca
     8521 ccttcctgag ctcttgatga gccagcacct ggactctgac ttaaaactgt accttcttta
     8581 tatattgagc tatattcata cttaacagtt cccactttta agttattttc ttcaataagt
     8641 tgttttgcat tactcaaagt aagtcccacc acattaggaa cagatgcctg acttgaacct
     8701 ttacttacga caacatttat agtatcaccc tttttgacat ttgtacttcc accttcaggt
     8761 gtctgtgata ttatacaatt ttcatcaact tcactattat actcttcact ttttacactc
     8821 aggtataatc cttctttttc aacagcactt tgagcttctt ctaatgtcat atttacaaga
     8881 ttaggaacag tcaaagattt attgccaaac cctccagcaa ataaaaactt atatgctaag
     8941 aatacttgag cacacaatat cagtatcaaa acagccgcaa ttacttttaa tcttcttcta
     9001 gattttgggc tttcctgttt tttcttcttt ttcttagttc tcttacttgg agtactctta
     9061 ggtctctgat tctttttagc tctcattatc tcttcctctt cttcttcttc ttcttcatct
     9121 tcttcataaa aatcatcata gtaatcttca tcatcatcca aatcagctac ttcaacaggc
     9181 ttaaccactt tttcaggagc tggttttgca agtgttggat ttacaacttt attaatttct
     9241 ttttcatcaa ttttcttagt tgcaaaatca tcatattctt ttataaaatc taaatcaata
     9301 tttttttcta tatactctat atcttctatc aattcttcag cagtttgata tctatctgcg
     9361 ctagattttt ctgtaagctt ttttattgta gttcttacac tttgaggaat cctaactttt
     9421 tcttctgatg taaagtctat atcatcatta atgtgttgaa gtgctataga aattggacta
     9481 tctcccctaa atggaacttt tcctatcaac atttcatata aaactatacc tagagaatac
     9541 aggtcagcat tattagttac aaatttacct ttagcttgct ctggtgaaaa ataatgcact
     9601 gaacctatta tactaccaat attagtcatt gttgaatttg aaacagcttt ggctatacca
     9661 aaatcagcca cttttactac tcttccttca tttgatatga gaatattatg aggttttata
     9721 tctctatgta taatcccctt tttgtgcgct gcactaagtg ccatagctat ttgttttgtt
     9781 atatctaatg cagtatattc atctaatata ccttcatctt gtataatctc ttttagattc
     9841 ttcccatcta caaactccat tacaatgtag tgaacttttc catcttcacc aacatcataa
     9901 acatttacta tatttggatg tgaaagactt gctactgctt ctgcctctct tttaaacttt
     9961 gttagaaatt catcatcatc tacaaattca ggtctaagta ctttaagtgc aacagtcctg
    10021 tttaataatc tgtctttagc ctcatataca aaggccattc ctccatcacc aatcttcctg
    10081 atgatttcat atcgatttcc taaaattgta tctcccacaa tatcaccgtc ctaattatac
    10141 ttttatcact ataaccgaaa cattatcttt accagaaaca tcattggcca tacttatcaa
    10201 ttcttcactt atattactag ttctctcata ttcataatct aatattaaat ctcttatatc
    10261 ttcatcatca ataaacccag ttaatccatc tgtcgcaaga agtattatat cacttttttt
    10321 gatttctttc ttaaaaatat ctacaacaac catatcatct gttccaattg ctctagttat
    10381 tctatttctc tgtggatgtc ttcttgcctc ttcttctgta atgacatggg ccatcataag
    10441 ttcttcaacc acggaatggt ctatagttat tttatcaaat ttttcatctg ttaacaaata
    10501 acatctgcta tctccaacat ttgccacata taagacatta ttatacacta tagccgtaac
    10561 taatgtagtt cccattcctt caaattctat atcttccata gattttttgt gcacaattga
    10621 gtttacatta ttataagcct gttttaatat atcatctata tagtctattt ttacattatc
    10681 atgttgtaat aagttttcct ttagaaaatc aattatattt tctactgcta atttactggc
    10741 gacttcacct tttttatgtc cacccattcc atcagctatg gcaaatattc ctattggacc
    10801 atgttctgta tctataactt ctccctcaca atagtcttca ttgttttttc ttatttttcc
    10861 tatatgggag gcacaactat aaaccatacg acttcctcct tacaaattac ttgtattttc
    10921 ttctcaattg accacaagct ccactaatat cagaacccat agacatccta actgtggcag
    10981 gtatattgtt cttctccaaa ctatccctaa atttataaat aaatgcttta tctggctttt
    11041 cgtattctct ttcttcaact ttatttattg gaattaaatt tacatgacat agcatacctt
    11101 ttaggagctt agctaatgcc tttgcttctt tttctgagtc atttactcct tttataagag
    11161 aatattcaaa tgtaaccctt ctatttgtct tttttatata atatctacat gcattcaata
    11221 tttcttcaat agaataagca tttgctacag gcataatttt tcttcgttct tcatcatatg
    11281 gtgaatgaag tgataaagct aaatttatag ctatttctaa gtctgccaac tcatacattt
    11341 taggtactat tccacaagtt gaaagagtta tatgtctata ccctatatta agaccatttt
    11401 tttcgttaat tatttttaag aattgttttg tattttcaaa gttatcaagt ggctcaccac
    11461 tccccatcaa tacaagattt gataccctct taccagtatc ttcttgaatt ttaataactt
    11521 ggtctaaaat ttcccatggc tctaagtttc taattaatcc atccattgta gatgcacaaa
    11581 aattacatcc catcctacaa cctacttggt tagaaacaca gacagtaact ctactatcat
    11641 aatccatcat cacagtttct attatatttc catcatctaa taaaaacaaa tatttttttg
    11701 tattatctac cttagattct aactttaatt ctatatctat attccctata catgaaacct
    11761 cttctaattt atttctcaaa ctttttggta tattgttcat atcatcaaaa gtttttgcac
    11821 ccttatatat ccaagagaat atttgacttc ctctaaaggg cttttcatct atagttttca
    11881 taaattcttt aagttcatct tcagtaaaat tttttaatac tatctttttc ttttccattc
    11941 tctcactacc ttattctctt taattttgct atgaaaaaac catctatacc atgaatatta
    12001 ggatatatct ttaaatatcc tttatcttga ttgtctaagt ctacatttac tgtatcaatt
    12061 ggaacaaatt caaagttgtg attttcttca acaaaagaag taactatatt tatattctcc
    12121 acatcttgaa ttgtacatgt actatatact aaagttccac caactttgac atatttagat
    12181 gcattttcta aaattttctt ttgtatagaa gtgatgtctt ttaactcttc ttctttctta
    12241 tacttaattt ctggtttacg tcttattata ccaaaaccag agcaaggaac atctgctaaa
    12301 acataatcaa atttacttat actattttca tcaatctcag aagcatcaaa tctttctaca
    12361 aacacatttt taagccccaa tctatttaca gtagagttta taagctttag tttatgctca
    12421 aaaatatctc tagctactac ttgacccgta ttgttcatta aagtagctaa gtgagtagat
    12481 ttccctccag gagcactaca tacatccaaa acaaatgaac cttcttttgg attaattact
    12541 ttccctacta tcatagaact tatatcttga attgtaaata aaccagcctt aaataattca
    12601 tttttttcta tatttttcat tttttcgact ttaatagctt cttctaccat aggaactttt
    12661 aaacatacaa tacccatttc acttaatttt tcaataagtt cttctctact tattttaagt
    12721 gtattcgttc ttatatagat actaggcttt tcattatttg cttccaacaa atcttcagca
    12781 aattcttgtc caaatttatc tatccagttt tttataatcc aagagttata ggaatacttt
    12841 atagataagt attttacact atcttcctca gttatgtcca ttatagtctc tttagctctt
    12901 atttcatttc taagaatagc atttatgaaa ccagaagatt ttttatcata tttcttagat
    12961 aattttacag tttcattaac tgctgcataa tctgatatac tatctaaaaa taaaatctga
    13021 tatgttccca ttcttaaaaa gattttaaca taagttgaca ttttttttac ctttattttt
    13081 gaaagtttat tgattatata atctaaataa tatttatttt ctattactcc ataaattaac
    13141 tctgttgcaa gtcctctatc catatcactt atttctaaat ttttaaaatg cttatttatt
    13201 gcaatatttg aataattgtc atttttttct atatcacata atactttaaa tccaacttct
    13261 cttgcatcca tataaatttc tcccttttat taaacataaa aaagggttgt ttctaaacaa
    13321 cccactttac ttttagtcat ttcttctttg agctattagt aacaatctaa gtaattgaag
    13381 tattgaaacc aatgctgctg caacatatgt taaagctgct gctgaaagta ctcttctact
    13441 ttgtcttgac tcactttcat ctataatacc taaattccca agttgaacga ttgctctact
    13501 tgaagcatta aattctacag gcaatgttat tatttggaat aacactgaag ccgaaaacaa
    13561 tagtatacca atttttaaaa atggaccagc cataataaat cctaaaaata ttaaaaacca
    13621 agatatactt gaagcaaaat tcactactgg tactaaacta cttcttattt gaagaggcgc
    13681 ataaccttta gcatgttgta tggcatgacc acactcatgt gcagctactg caacagaagt
    13741 tatagatgtt ccataatata catcttctga caaccttaca gcctttcttc ttgggtcata
    13801 atggtcactt agatgccctc taaccatttc tatctttaca tcatacaaac catttgaatc
    13861 aagtactctt cttgctgttt gctctccagt atatcctcga cgtgtattta ctcttaaata
    13921 cttatttgtt gttgaactca ctttaaattg tgcatatata gtaagtaata tagctggaat
    13981 taatacaacc attgttgggt caaatcccca aaaaccgcca tatacagggt acataaataa
    14041 cactctccta aaatatattt ttattataca tcctcttaat attcacttaa cttaaaaaca
    14101 gtttaattac aaattaatat attttttcct gttaaaaagt ctatagcttt ttcaatatcc
    14161 atcatgtcta cattagtatt tacacaaggt ccattaggtc ttttgttaaa tacacctaaa
    14221 acaggaattt tcttaatatc ttgtattcca gatgtcaaat ctctttcaca tgcaacagct
    14281 acaacagctt taggtttatt atccattata atttttctag ctagggttcc tcctgtagct
    14341 acaaatattt ttacattagt tttttccttt agcttaatta attctcctac gttgcatctt
    14401 ccacattctt tacaattatc tatatcatta gtaactttta atttacaact attctcttga
    14461 atacaatgag gtattaaaat tattatatcc tctggattaa aattatattt attactgtat
    14521 atatattcat tatttaactt tatatatatt cttctaatat cactttttga cataccaaat
    14581 gatgatgcaa tgaaagatat aataggaaat aatccactga caatattaaa atttattttc
    14641 attatatttg agttaacata ctttccttta attactctat aagtaactgc tgttgaacat
    14701 agtattataa gtattagtga tattattata atatttatca tcaaggagaa tatttgagtt
    14761 aaacttatta taaaaaaatt aacaactcct atcaaaattc ctaatattaa aatcaaaatc
    14821 ccaacacttg ttaaatactt ctttatatct gtcatacttt tcacctaacc caatactaaa
    14881 ccagtttcta tagtattccc ttttatatat tcaccaacca acattctctt tttattcggc
    14941 atctgaattt cttcaattaa aagtacatta tctatagtac ttacttttat cccatctttg
    15001 cttacatcaa ttattgtacc tacatctttt gtgctttttt catctaacac tttagttttc
    15061 caaactttca ttatgacatc attatagata gtataagcac ttggccatgg atttactcct
    15121 ctaactaaat tgtgtatttc tctggcactt tttgaaaaat ctatgttacc taatgattta
    15181 ttcattattg gcgcatagga aaattcttca tgattttgta cttctcttgg agcatttcct
    15241 tcttcaatta atcttaatgt ttcttttaaa gtttctgctc ctatattcat cattttatca
    15301 tgaagttctc ctgctgttat attttcatca agatttactt ctgtctttag tatcatatct
    15361 ccagtatcta acccttcatc catatacata gttgtaacac cagtcttttc ttcaccattt
    15421 attattaccc aatttatagg tgccgcacct ctatattttg gaagtaaaga aacatgaaca
    15481 tttatacatc caagtttagg aatctctaat atttcttttg gaagtatctg tccaaaagct
    15541 acaactacta ttacatctgg atttaaagat ttaattttat ctataaattc tttatctcta
    15601 gcctttactg gttgatatac aggtatatta ttttcaattg ccagttcttt tactggactc
    15661 atacctagtt ttttacctct tccttttggt ttatctggtt gagttacaac acccaatatt
    15721 tcatacttct catctattat tttttgtaaa catggaactg ctatatcagg agttcccata
    15781 aatactattt tcatcaaatc aactcctact acttttctat tttgtcaaca aataaaattc
    15841 catctaagtg gtctatctca tgacaaaatg ctctagctaa aagttcttct ccctctaact
    15901 ctattgtttc gccatttcta tttaaagctc tgacctttac ataatttggt cttctaacat
    15961 ttccagcttc tccaacaaca cttaaacatc cttcctcatc tatttgctcc ccagatgttt
    16021 ctattatttc aggatttata agttctataa gctcttctcc aatatctatt actactactc
    16081 tttttaatat tccaacttga ggtgcagcca gtccaacacc atctgcatca tacatagttt
    16141 ctgccatatc atctaataat tgtattatct tttcatcaat tttttcaacc ttttttgatt
    16201 tttttcttaa tactggttct cctatttgaa ctatttgtct taaagccatt ataattcctc
    16261 ctaaatttat aatacactgt ttggattaat atctatagat acattgatat tcttatcaaa
    16321 tatcaaatct cgttttgtta tacatatata ttttattata ccctttaaga gattaatttc
    16381 aatattttca tctttaaaga gtatttgcca tctataattt tgatttattt ttgatattga
    16441 acatggattt ggacctaata taaatcctaa atcatttata cccctttttt ctaataaata
    16501 aatcaatgat gcatgcatat tttttatatt ttttatcact aatctttcat cttcaccact
    16561 aacaactaca cttaacatat tattaaaagg agaatatcca aatgcttttc ttatttttat
    16621 ctcatcttca taaaaaccct cataatcata ttcaatagca tgcttaatag catagtgttc
    16681 agtgtcataa gtctgaagta ctacttttcc ctctttatca gctctccctg ctcttccaga
    16741 gacttgagtt atcaattgaa atgtagtttc aaaactttta aagtctggaa aatttaatat
    16801 catgtctgca gacaaaatac ctactaaagt tacattctca aaatccagcc ctttacttaa
    16861 catctgtgtt ccaataagta catctgcttc tttatcttta aatttattta gtatctcatc
    16921 taaagcacct ttttttgaag ttgtatcttt atccattctc aaagttttta tatctggaaa
    16981 aatatatttt aattcctctt caattttttg agttccaact ccaaatggct taacataagt
    17041 acttttacat tcaggacact cttttggaat ttctttctca tatccacaat agtggcatct
    17101 tccagtattg cttttcttat gataagttaa agatatatca caattttcac attgaaacac
    17161 atatccacac tttctacaag atacaaaatt tgcataacct cttctgttta aaaatagtat
    17221 cacttgatta ttttcttcaa tcgcataacg tatttctttt tgtaatttca aactaaatat
    17281 acttctattt cctttatcta attcatcttt catgtctacc acttcaatgt ttggaagagg
    17341 cttatcattt gctctacttt ttatatttat aagtttgtat tctccagatt ttgccctata
    17401 atactcttca attgagggcg ttgctgaacc taaaataaga gttatattat tttttaatgc
    17461 cataaatcta gcaacttcta tagcattaaa ttttggattc ttctcagact tatatgatga
    17521 ctcatgacat tcatcaatta ttattaagcc taaggaatta aaaggtgcaa ataaagctga
    17581 cctagctcca attaacactc ttactttacc tgctttgaca gctttgtaaa catcatgttt
    17641 ttctccttca gatagtttac tgtggaacac tccaactaaa tctccaaacc tactctttag
    17701 cctatctatt gtttgaggag ttaatgctat ctcaggtact aagaatatac tgtctaagcc
    17761 ttgatttaga gcaaattcta ttatttccat gtaaacttca gtttttccac ttccagttac
    17821 tccatgaatt aagtatggtt ttttatcatc tacaaacata ttcgacttta tctcatcaac
    17881 tgcttcttgt tgttcattgt ttaattttat actttttagt acggatttgt aaatactttt
    17941 tggctctcta taataatcct taaattctag ggttattagc tttttcttac ttagagaatt
    18001 tatactttgt tttgaagcat ttaataaatc caataaatca tttatctcta cattttcatt
    18061 atttttaaga aaacttaata tctctttttg tttacttccc acatttattt tattttgctc
    18121 tatataatcg tcaatcttat cactttctaa actcaaagat atataacata ctttcttttc
    18181 atttttgtga tttttatatt cccaacataa ttttataatt ccatttttat tcattgtgta
    18241 caaagaacta tttaagtttg gtatatattt taacttatca accttaattt tacctttact
    18301 atcaatcact ttctctacta tttctctatt tttatcactt aaagttgata ttttttcctt
    18361 caattcaatc tcatttaatc cacttaaact ttcacctaat aggactactt tataattatt
    18421 taatttgtat ccctttggat atatcaaatt tatacattct atataagtac acaaatatct
    18481 atttttcatc caataaacca actctaaatc ttcttttcta aatattggat tttcatctaa
    18541 tatatcaata atttctttta tttttatttt ttcatctaag ctatctgtaa attgaaatac
    18601 aaaggcttct gttggtttat taccttttcc aaatggtact aatattctgt gcccgatttt
    18661 tattacatca gataaaaata caggaatttg atatgtaaat aaattatctg tatatatagt
    18721 attacttcta actattacct ttgcatattt tttcattata aaacctctac ttaaatttaa
    18781 tatattgttc atattttaat atactttaca agatattaaa atctcagaat ttataagcaa
    18841 tttcaagtta ttaatattaa ctttatttta ataattaact attaattata ttttatactt
    18901 tatgacaaaa taaatgctga aaactacaat ttaactgaca tttgcgagtt tttactcgct
    18961 aaaattaaat tgcagctttc agccacttta gctgtgataa aatacacaaa tatcatattt
    19021 aaaaaaggtc ttatttctca agcaacgctt taactttatc taaaataaca ttggctactt
    19081 cttctttttt catcttagga tactcagtaa tatttccctc tacatctatt atttttacaa
    19141 tattagtatc agctctaaaa ccagcacctt ctttagttaa atcatttgcc acaataaaat
    19201 ctaaattttt cttttttatt tttcctttgg catgttctat aaggtcatta gtttcagctg
    19261 caaatccaat tagtatctta tcttctttta tttttcctat ttcataagca atatcataat
    19321 ttctacttag ttctattgat aaatcatcat tcttcttttt aatttttttg tcagaataag
    19381 tttttggttt ataatctgca actgctgcac tttgaattac tattttattt tcacttaaat
    19441 ttgatactac tgcttcatac aaatcttgtg ctgactctat ttgaacaaat tttttaagat
    19501 tttgtggaat agaaagattt gtaggacctg atattaatgt tacatcagca cctctagcta
    19561 tagcttctct tgctatagaa tatcccattt taccagaaga cctattagtt aaatatctta
    19621 ctgggtctat actctctaca gttggaccag ctgtaactat catactttga ccttttaaat
    19681 cttgctcttt agtaagatta gtcaaaactt cctctactat tatgtctgga ttagctaatt
    19741 tgcctgttcc aatatctcca catgcaagcc tcccactttc tggttcaata aatttatatc
    19801 caaagctctt taaagtttgt atattttttt gaacaattgg attttcatac atattagtat
    19861 tcatagctgg tgctataaga acctgtgctt tggttgccat aacagtagtt gtcaacatat
    19921 cgtcagctat accatttgca atcttgccta taacatttgc acttgctgga actattaaga
    19981 ataaatctgc tttttttgct aatgatatat gttcaacttc ccaagttttt ggttcttcaa
    20041 acatatcttc tactacataa ttttgactca gcgattgaaa tgcaagtggt ttaacaaatt
    20101 ctgttgctga ttttgtcatt ataacatgga tatttgcatt tagttttttt aacttactta
    20161 caacatcaca agccttatat gcagctatac cgccacttat gccaattaca acagttttat
    20221 cttttagcat gaccttactc ctcttctgaa atttgttggt gttgctcagc atgatgtcta
    20281 gcttcttcta tctctatttc ctcttctgtc aataatctat atgttatttt tccacttgca
    20341 acttctttag ttgctacaca tacaggcttt tccttagttt tatttgaaac atatggttct
    20401 tctccatcaa ttaattttct tgctctcttt gaaacagtcc ctactaaata gtatctgttg
    20461 tctatttttt ctagtacctc attgatagat ggttttaaca taattataat tcctccttaa
    20521 atttctctat tataatattt ttatatctgc taactttatt cttttcagat gatattatac
    20581 cttctatttc tttagcagat gtttttacat cttcattaaa tataaaataa tcataatcct
    20641 taatcatttt aatttcttca tatgcacagc taaatctctt ctctatttca gcttctgttt
    20701 ctgtacctct tccaacaatt ctatttttta attcttcaag tgatggtggt agtacaaata
    20761 taaatactcc ctctgggcaa acttctttta tttgttttgc tccttgcatt tcaatctcca
    20821 gtaatacatc ttgtcctttt tctaaacatt ccattattgc cgcttttgga gttccataaa
    20881 aattatcata aatttgagca tactccaaaa actcaccttt ttctatcatc tcttcaaact
    20941 tttccttact tatgaagaaa taatttactc catcaacttc tccagttctg ggttttcttg
    21001 tagtagctga tacagaaagt tttattgtat cattttcttt aagtagttct ttgcaaatag
    21061 taccttttcc tgcacctgat ggtcctgata caactagcaa taatcctttt ttcacaaaca
    21121 tttaagcatt ccccttctaa tattgtattc ttactctata ttttgtattt gttctcttat
    21181 tttttccaat tcgcttttta cttctactac taaattagtt atatttatat cagatgactt
    21241 cgaacctata gtgttagttt ctctattcat ctcttgtatt aagaaatcta tttttctacc
    21301 tatagagtcg tctttaaata tagtattttt aagttgccct atatgacttt taaatctaac
    21361 tatttcttct gttatactgc ttttatctgc atatattgca acttcttgtg ctaatctatt
    21421 ctcatctatt atacttgggt catccagtaa ttcacttatt ctcaaattca atttttctct
    21481 ataatcttct acaacactac ttgaatattt ttcaatctct tctatatgat tttttaataa
    21541 atcacatctc atggcaatat cttcagctaa ttttttacct tcttcacttc tcatttctct
    21601 taatttgatt aatgcatctt caactgcttg attcaacatt gaccacaata aatcttcatc
    21661 ttcttccttt tcttctattt tcacaatatc aggaaacttt gcaacattca taacgctaat
    21721 atcatctata atatcaaatt tattttttat ttcttttagt atgtctatat actgagatgc
    21781 taatccctca tcaaaattta agtttacatc ttctttgcct aataagtcaa actttatgta
    21841 taaatctact cttcctcttt ttacataatt ttttactaaa tttctaactt tatcctctaa
    21901 aaatgaaatt tttcttggaa gacgtatgtt tatatctgaa tacttatgat ttatcgtttt
    21961 acactctact aaaaagtaat aattatcatc cttgtattct cctcttccaa aaccagtcat
    22021 acttatagcc attaattaaa cctccaaaac accttcacat atttttactg ctggacctgt
    22081 catataagag aaatctccta catctatttt aactgttcca ccttgagacg ttacatttac
    22141 actactttct accatattaa gtaaattgca cactattgct gatgcagtca taccagtacc
    22201 acaacctaaa gtatatccac aacctctttc ccaagtataa acatgtatat tatacttatc
    22261 ttctaccttt acaaaattta cattcgtttt ttcaggaaat attttattat tttcaatctc
    22321 ttttccatat ttacaaacat catctacatc taattcatct actataacta ctgtatgagg
    22381 aacccccatc aacatagaag taactttaag ctccctatct aaaaccttta tactttcttg
    22441 cacaaaacgt tctttatttg taagtacagg aacatcctta gctataaaac ttccttttcc
    22501 catattgact cttatagagt ttatttctct attggtaaga tttattttta tatcttttat
    22561 accatctaat gtatctactg taaaactttc ttttttaact atacaattat cataaacaaa
    22621 tttacaaaaa catctaaggc catttccaca catagctgct cttgaaccat cagaattata
    22681 atataccatt tctacatctg caacttttga atctttcaca actaacaatc catccgctcc
    22741 tacagaaaat cttctatggc atactttttt tgctaaagca gtataatcag atggatttat
    22801 ctggtcgaat cttccatcta ttgctataaa atcatttcct attccatgta gtttccaaaa
    22861 tttcataagt atcgctcctt cgtaaattaa cgcttaaact attataacat aaaaaaattc
    22921 ccataaactt ataatttatg ggaaaagttt tgtaatgaca actataattt gaggatgtga
    22981 gggagagttg tcattacaat tagggtaatt atattatgtt tttaagctag ttctttctat
    23041 gtttatatat taatatttct atatgaattt tatgtatgtt ttatttaaac tgtctatgaa
    23101 ttttttaatt aaacatctgg taaccagcta taatcctttt agtagatatc tattaacata
    23161 cacctattaa ttctttaagt gtttttaaaa catagtctaa atcttcctta gtaatatcta
    23221 catgagttac aaatctatat tcaccatttt ccattttatt aatttttata tttctttcaa
    23281 aaaattcttt tattaaaaca tcctctttta taatatcttc actcatctta aaaaatacca
    23341 tacttatatc tggattaaca ttgtttactt caatgccatc aattttattt aattcacaag
    23401 ctaaatattt tgcattttca tggtcttcat tcaatcttaa agtcattttt tcaatggcaa
    23461 ttataccagc tgctgcaact atacctactt gtctcattcc cccacccata actttttgct
    23521 ttcttctagc ttttgctata aattctttac ttccagcaag tatagaaccc attggtgcag
    23581 ctaaaccttt tgacacacag aacataacac tatcacaaca ctttgctatt tctttagctt
    23641 ctacacccaa agttaatgct gcgttaaata atcttgctcc atctaaatgt actggtaagt
    23701 tgtgtttctc agctattttt ttaatttctc ttaaattatc aagactcaca acagttccac
    23761 ctgaatatgc attttctaca catataagcc ctgtctgtgg ttcatgtatg tcatctcttc
    23821 taattgcctt ttcaacaagt tcaggattta gagctccact ttcgtcttca tctatagttc
    23881 taagctgaac tccagctatt acagcaggtg ccccaacctc atgagttaca atgtgataac
    23941 ctttaccaat aattatttct tgccctctta ggcaatgagt aaatagagct aattgattcc
    24001 caaaagttcc acttggaagg aaaatagcat cttcttttcc aactttagct gctgctaatt
    24061 cttcaagttt atttacagta gggtcatctc taaagattga atctccaaca ggagcctttg
    24121 ccatagcttc tctcatttcg tcagtaggca tagtaacagt atcacttctt aaatcgataa
    24181 atctcatttt ttcaaccttc tttcatattt atatactata gaaccttaaa attgattttt
    24241 tctgtaaagt tcatatactt tcatagctta tactctacac aataattata ctatatttac
    24301 tttatattta atctacttta aattattaat tttaaaaata aagtttcctc tctaataagt
    24361 ataaataatt atacaattta aatattaatc tctatcgttt aaattccccc tagtcacaaa
    24421 gcgtatatta tgtgtataat taatttactg actcattttg gtcaaagaaa ggggtgctat
    24481 aaatggcatt agatggatta gttatacatt caatagtcga tgaattatca tcaaaactta
    24541 caggtggaaa aatagataag atacatcaac ctgaagatga tgaggtaatt tttaatataa
    24601 gaaataataa agaaaatttt agattggttt taagtgcaag tgcctcaaat cctagagttt
    24661 atcttacaag taattatcaa aaagaaaacc ctctaaaagc gcctatgttt tgtatgttac
    24721 ttagaaaata catacaaggt ggaaatatag tagaaatttc tcaaataggt tttgaaagaa
    24781 taataaaaat aagtgttgaa tctttagatg aattaaaaga aaagacagtt aaaaatatta
    24841 tgattgaaat tatgggaaga catagcaaca taataataac tcatggtgaa gaaaataaaa
    24901 ttatagattc tataaaaaga gtccctttta gcataagtag agttcgtcaa gttttacctg
    24961 gtcatgatta ttcattacca ccagaacaaa acaaactcaa ccctcttgat gatatatcta
    25021 aggatttgtt tattaaaaac ctagaagaat tggaaggacc tatctttaag tctatatatt
    25081 ctaaatttct aggtataagc cctattatag ctaaagaaat ttgctacaga gcaggtgtaa
    25141 atcaaaatgc tatcataaag gatatatctg atgaacaatt tgattcttta cacaaagtat
    25201 tttgtaattt attcaatgat ataaattcta acaaatactc cccttgcatt attatagata
    25261 aaaaggtgga taaggtagtt gatttcagtt gtataaacct tacattattt agcgatttat
    25321 catatataaa taaggatagt atgtctcgta ttctagaaga tttctataga actaaagata
    25381 ttaaagacag aattaatcaa cgttcatctg atttaaagaa gagtatatct gttaagcttg
    25441 atagacttta taataaactt aaaaaacaag aagaagaatt atcagaatcc gaaaatgcag
    25501 acatttataa gataaaagga gaattgatta cttcatatat ttatatggta gaaaaaggta
    25561 tggaaagtat tgaaattgct aacttctatg atgaaaattg taatgatgta acaatagaat
    25621 taaataaaaa cttgacacca tctgaaaatg ctcaaaaata ctttaaaaag tataacaaaa
    25681 tgaaacatgc taaagtcgag atatctcatc aaatttcctt aaataaggaa gaaatagatt
    25741 acttagaaaa catagtttta agtattgaaa actgcgaaaa tttagctgaa ttacaagata
    25801 taaaagaaga acttgctaag gttggatata tcaagactca aaagaaaaac agtaaaaagg
    25861 atactatccc atctacaaaa cctcatgaat ttgtgtcttc tgatggattt aaaatattgg
    25921 ttggtaaaaa caataagcaa aatgactatc ttacactaag acttgctgat aatgatgact
    25981 tatggatgca tacaaagaat attcctggtt ctcatgtaat aataaagtgt gctggtaagg
    26041 aagtccctga taatactgta tttgaaggag ctatgctagc agcattcttt agtaagtcaa
    26101 aactttcttc tcaggtgcct gtagactata ctaagagaaa aaatgtgaaa aaacctagtg
    26161 gttctaaacc aggtatggtt atttatgaaa ctaacagtac tatttatgtt actcctgaag
    26221 aagaaacagt agccaagctt aaggttaaat ctgaataaat tctgcactaa atacaaaata
    26281 aatgttagac aagcacacat tatcattcta aataaaaata gtggccttct tataaagtac
    26341 aataataagg ccactttcta attagcttac ttttaatttt ctaacaaaat acttttctgt
    26401 tttaataact atacttatat tcccaacaaa tattttcagt cttcttatat agatgtaatt
    26461 aaaaagttaa cactaaaagc tattttttaa tttagtgatt tgttttagta attcattata
    26521 ttctgattca tacaattctt tttttagatt atctttttct atagataaca tagatataac
    26581 agttgtaagt ctattttcta atactagaag attctctttt ttgatttgct tgtctctact
    26641 aggagttttt ttatttctgc taattatata ttcgtcataa ttacaatcaa actcccttat
    26701 tttagcatct tttatctcta taatatagtt acaaatatgt gatacaaata ctctatcatg
    26761 agacactata agcatagttt tatcagtatt tatcagtgcc ttttccagtg cttccataga
    26821 ttttatatct aagtaatttg ttggttcatc aaatactaaa aaattattat cttccaatat
    26881 tattttacat agtgctattt ttaccttttc tccaccacta agtactttta cttttttgta
    26941 gacatcatca cctttaaacc caaataggct taagtttatc cttataaaag attcatcaaa
    27001 tgaactatta actttagtat tgtatagaac actcttttcc tcatctaata aactttgatt
    27061 ttggtcaaaa caacctactt ttaccttgtt atttattttt atattgtcat ttttatcagc
    27121 caatatttct tttatcagag tactttttcc acacccatta tcacctaata atgctatctt
    27181 tttacctctt tttatcttaa aggaaacatt atctaacaag agcttatttt ctaactttag
    27241 agtcatatct tttacctcta atagattttt gctaattatc tccataccat cttgaatctt
    27301 aatgttcatt tctttagaaa ctttaggttt attctttacc tcaagtttat caattctgct
    27361 ttttatagcc ttcacatttg agtccaactt tttcttatta gtctgaccac ccattttgtg
    27421 caacctagct tctgaattac ccattctttt tggtgtcttt cttatgccat cacttaaagc
    27481 ttttttatat aaaagagctt tttcaagcct ttttttctct ttaacatatt cttgatactc
    27541 aaaatcagct ctttttactt cttcatcttt ttgcattaaa taatcagtgt aattgccttt
    27601 gtaagctttt agttttccat cttctagttc taatatagtt gtacataact catccagaaa
    27661 acgtctatca tgagatatta ataataatgc tccctcatgc cttttaaaca tatcttccaa
    27721 aagcccaatg ctttttttat ctaagtttga agttggctca tcagctatta tgatattttt
    27781 cttattactc agcgcatctg ctatttttaa cttaaccttt tcccctcctg ataagtattc
    27841 ttcaaattta tctggagcat ttaaaagact cttctcctta ctatgactac atgtttcaat
    27901 attattttca ctctgactta tataagaata gctttctgta aggtaagtat atccttcatc
    27961 aatttctatt tcacccaata ttgcttttaa taatgttgtt tttccttgac catttgaacc
    28021 aactagacct attttgtcat tttctaatat ttcaagttta tctatatcca aaattaattt
    28081 atctgcataa tatttcttta aattctctac ttttactaac aacataaaaa atccctcctt
    28141 aacacactta aatataagga gagattctat aaataggcat aacaaataaa cgtatagact
    28201 ataatctact ctccttaatt taagcattgc aaataaagcc atcaaaatag gcttttcaaa
    28261 aattacaata ctttaaatta taaaaaatag attatattct cattgtccta gttaccttgc
    28321 cctttctttc atttattttt aatcattata tcaacattaa ttacaaatgt ctatagaaaa
    28381 ttaactaatt ttagttattc aatatacaat ttattcttta aatttattct tcttcaactt
    28441 taaaatcctg attatttaaa attctgttta atacaattcc aacaattgca gctaaactaa
    28501 gaccagttat ctttactgta cttgttatag ggattccaat agaaatgcct ttattagata
    28561 aatatgttgt tccaagacct attaataata taactgctat tattaatata tttatcttac
    28621 tttctacaca agaactgtcc cttatagttt ttaatcctac ataagttatc attgaaaata
    28681 gcattatact aattccaccc ataactgaac ctggtatact ttgaagaaat ccaccaaact
    28741 ttcctacaca tgcaagcaat atagcaaata ttgctgtacg tctaagtatt gaagggtcat
    28801 agtttttagt tatagcaagt actgctgtat tttctccata agttgtattg gctggtcctc
    28861 ctaaacaccc tgcaactata gtcgcaaatc catccccaag taatgttcta tttaatcctg
    28921 gattttctat aaagttttta cctactacag caccattagt agtcatatct cctatatgtt
    28981 ccataaatac agctaataca actggagata taatcacaat tgctcctaaa ctgaacttag
    29041 gaactgtaaa gttcggtatt gcaaataaac ttgcttctgt aatagtagtt atatctacta
    29101 accttaacag taaacagatt atatacccag aaaaaacggc tattaatatt cctaattgtt
    29161 ttataaatcc tctaccaaac ttattggtta aaattgctat tgctaaagtt ataaatgcta
    29221 ttacaaaatt agctgaagcc atactaaaag ctgttggcaa taaatttaat cctataacta
    29281 ctatcatagc tcctgtaact tgtgcaggaa aatataattt aattttattt acaccaacta
    29341 tttttactat aatcgacatt ataatataaa ttaatcctgc tactattata ccaccttgag
    29401 catatgctaa atctccacca tacgcctctt tagctgctag tataactggt ataaacgcaa
    29461 aacttgaacc taaaaaggca ggtacttttc cttccgtaca aaaatgaaat atcaatgtac
    29521 caacaccagc acagaaaatt gcaactgacg gattaaaacc tgtcaaaatg ggaactaata
    29581 ctgtcgctcc aaacatggct aatagatgtt gtaaagacat tattgttttt ttcataaaaa
    29641 cctccaaaat tattatatat aaataaatcc tctgtttaat taatatcttt taattaatta
    29701 tcattcccta gattctattt tttaacctct atatacttat tatataattt atcattattt
    29761 tacattttac gacatcaaaa actcaaataa aggagaataa aaactacatt aagactcaca
    29821 tccataaaat tataatattt attttgtgca atctatgtaa gctttatcct cctccctgac
    29881 tattcaatgc taagctttta tataatactt aaaactaatt attctttgat tgtaactgaa
    29941 tcttctccat caatttctaa taaactgaca gatataattt catgtctaga agtaggtaca
    30001 ttttttccta cataatctgc tcttataggc aactctctat gccctctgtc cactagaact
    30061 gctaattgta ttgatttagg tcttcctata tctataatcg catctagtga agctctcaca
    30121 gttcttccag tgtataaaac atcatcaacc aaaactactg tcttatcatt tacattaaaa
    30181 tctaaataag ttcctttaac tacaggttca acatgaattt tttccaaatc atctctatat
    30241 aaagttatat caatatctcc tgtatctact tttgttcctt ctatttgttc tattttctta
    30301 gaaattctat ttgcaattgg cacacctcta gtttttattc caactaaaac tagattttct
    30361 acacctttat ttctttcaat tatttcatga cttattctag taattgctct tgctattgcc
    30421 ttttcatcca ttaattgagc tttttctacc atatcataat tattgtcaaa gatataatat
    30481 atctatttga caaatctact cctttctttt aattttttat aaatttattt ttatttaaaa
    30541 tttcaattta aacaacaaaa aacttctcat cacaagacaa gaagctctta tataaaaaat
    30601 atacagtgta atttagcaat tgtcttacga tatctctgta tcgtttaaag ataatttttt
    30661 aatctatcaa ctcaaattat atatttatat ttcaaatttt tcaagctttt ttaaacaaaa
    30721 tactttaata taagatattt tatttaaatt tcactaaatt tatttactta tataaaaaca
    30781 aaaaatgatt tataagtatt atacaaatgg ttattttatc ttcttaatag cttccttaaa
    30841 atactcaggt aattctgaat taaattcgat atattcctta gtggtaggat gtataaatcc
    30901 caacttctta gcgtgcaaag tttgtccttt taatttaaat ttagtttctt ttggaccata
    30961 tacaaaatca cctaaaagtg ggtgatttat agacaaagca tgaactctta tttgatgagt
    31021 tctaccagtt tcaagcctag ctctcatatg tgtaaactta tcaaatcttt ctacaacttc
    31081 aaaatgagta attgcatttt ttccatcctt tactacagcc atttttaatc tatccttagg
    31141 atttctccct ataggtttat caacagttat tttatcttct ttaaccacac catagcatat
    31201 aaattcatac tctctagtta tagtatgttc ctttaactgt tctgctagaa aattatgtgc
    31261 ataattgttc ttagctatca taagaagacc agaagtatct ttgtctattc tatgaactat
    31321 accaggtcta ataactccat ttattgacga caacttatct ttacaatgat ataaaatccc
    31381 atttactagt gtattttcat agtttccagg agcgggatga acaaccatat cctggggttt
    31441 atttattatt aagacatcat catcttcata tacaatatct attggaatat cttgaggtaa
    31501 aacctctaat actttaggtt ctggtatctc tataacaata ttatcttcct ctttaactag
    31561 atatttagct ttttctactt tattattgac ctttactttt ccatctttta taattttttg
    31621 tatataactt cttgacatat cacctaattg ttctgctaga taaatatcaa gtctaacttc
    31681 ttcttcctct tcaagtacta aaaactcttt tacttcatcc attatatcac ctacttttac
    31741 tttcaaaaaa taaaacatat atacataaaa acacagttcc tacaactaca aatacatcag
    31801 ctacattgaa tacatattcc catataactc taaagtcgaa gtaatctacc acaaaaccta
    31861 atcgtactct atcaattaga tttcctaatg caccagatat aattaatata attcccaacc
    31921 ttccaaatat atgtactttc cttgtattaa gatagtataa tccaaatact gttgcaagta
    31981 atgcaacaat tataaatatc cactgattat tttgtaataa tccaaatgct gcacctctat
    32041 tttcgacata agttaaatga aatacattat ttattattgg tattgttgaa acatctacca
    32101 aattattcaa tacccatatt ttagacagtt ggtctaagcc tatgagtaga attattatta
    32161 atatatatag caatgatttt tccccctata aattatttaa aaaccaattt tatttgaaac
    32221 acactattat tattatacaa aaaaaatgct ctaaagagca actttattaa aattgtattt
    32281 tcttaataaa gctagagcaa aatttaaaat agtaccatct tttttatatt gcagattgct
    32341 acatatttaa aatcatatta ttatgtataa aaaatttaaa aataacatac taattaatac
    32401 tttttgtgtg ttttatagat taaagattaa tgatgtctgt actttaaact tacaagcacc
    32461 actaatcttc aaacaaatat ttttactttt gcacttgctt atttttatct aaatgttgta
    32521 taagttcaga tactgttacg aatttatatc ctctctcttt taatttagga attactactt
    32581 ctaaagcttg tatagtttgt gatttatcat ttcttttagt gttataatca tgtaaaagta
    32641 ttatattacc attttggacc tttgtaagaa tagtatttat aattgtatct acaccagggt
    32701 tagaccaatc tctagggtct aagtttgacc aaagaattat attcatgcct ttagaaacta
    32761 taatatcaca gacactttta ctcatggctc tatatggagg tctaaatatc tttggttcta
    32821 ctcctattac actttttata atattttgtg tatctgttat ttctttttca acactaccat
    32881 aactattttt tgcaacattt atatgtgtaa atgtatgatt ccctatttca tgcccctcat
    32941 catattgcct ttttaataat tctttgttat attctacttt ttctccaact acaaaaaaag
    33001 ttgcttttac attatttttc tttaatatat caagtatttg tggtgtaaaa tcctcatctg
    33061 gaccatcatc aaaagtcaat gctagaatct tttcatcctc atttcctttt tttattatta
    33121 catcttcata atcgtctttt atattgtcat ttaaagtata ggtttctttt gaactatttt
    33181 gattatgtgt attatggtta tatgtatata cacctactat ggataataaa catatacata
    33241 taccccatat tgctttcttc atgtcattac ctcttttcta attcattcta acaatatatg
    33301 atgtcaggct tctaatctct caatgttcca tttttattta aatcagttaa atttgttagg
    33361 tcttctgaat aaaagttaga atcttctaca tcaggatttt gcattgcatc acctaataaa
    33421 gcattttctt tttttgcaca attgttacat aaatttgtat caggtaatat atctaatctt
    33481 tctaaatcta tttgattgtg acataaatca caaaacccat atgttccatt ttctatttta
    33541 gataatgctg aatctatttg atacagtctt ccttcttcat gatttattaa actattttgc
    33601 atattttgca tatataaatc cgttcccata tctccaatat gattgtcata actagataat
    33661 tctcctgatg aatatttttc actagtatgt ttagtagtgt ctccaaacaa ggtattatct
    33721 ttcatatcta cgactaaatt agttaacttt tctctttctt ttaataattt atctttgtat
    33781 tttttagtat ccattttcaa cacctctaat tttaatattt atatcataaa aatagtatgt
    33841 ggaaaactaa attaaaaata caataaaaaa caagatttga ttttttcaaa aaaaattgta
    33901 ggtaaactta aaattcacct acaaattctt tacatatatt tttaatataa tgtttttagt
    33961 ttttgataat aattacatta acttgctaat ttatgagcat tttgctcttt tatatcctta
    34021 gcttttttaa aaaataaaag tccaaggatt attgcaacta caatacctat tccattagat
    34081 atattaactg gaagtctaaa tccttctttt gcctgcatta tataactaaa tgttactaca
    34141 gtcataaaaa gtgctggcaa tgtagttatg taatgatttt tatttgcttt gactaaatat
    34201 gcagaaccag tccacaacat tatcatagca agtgtttgat ttgcccaaga gaaatatctc
    34261 caaattatat taaaatctat aaatgtaagg gcaaccccta cagcaaataa tggcaaagct
    34321 attaaaaatc tatttttaac tgcatcttga ttataattca ttgcatcagc tattgttaat
    34381 ctagcacttc taaatgctgt atcccctgaa gttataggac atgctacaac accaagtatg
    34441 gccaaagcgc ctccaatagg tcccattaat ccaacagata ctgtatttac aacagttgct
    34501 gctccaccat cattaaatat tacattaagc tgaggtatac catggaaata tgacatagct
    34561 gctgctgccc aaactaaagc tattatacct tctgcaacca ttgacccata aaatactggt
    34621 cttgcctcac tctctttctc aacacatctc gccataagtg gtgattgagt tgcatggaaa
    34681 cctgatattg ctccacatgc tattgttata aataagtatg ggaataaagg tgttccattt
    34741 ggattcatat tttgtaaatt tatatttggt atgttatacc cctttattat taatcctcca
    34801 gctacaccta ctgccataat taataatgct gctccaaata aaggatatat tttaccaatc
    34861 aatttatcta ttggtaaaac agtagccata atataataaa gtattatcac tccaacccat
    34921 atttgtgcgt ttacacctgt caatgtcttt aataatcctg ctggacctgt tacaaataca
    34981 actccaacca atactaaaag aataacacta aatattctca ttgctatttt tgcaccattt
    35041 cctagataaa gacctaccaa ctctgatgca gatgtacctt tgttcttcat tgataaatat
    35101 cctgaagcaa aatcgtgtac tccacctgca aagattgttc caaacacaat ccacaaaaat
    35161 gctgatggtc caaataacgc cccttgtata gctccaaata tcggacctaa ccctgctata
    35221 tttagaaact gaattaaaaa gattctcttc catttcattg gcacataatc tactccatcc
    35281 ctacaagcta tagctggagt ttggtttttg tcattgattc caaaaacctt ttctacaaaa
    35341 gtaccataga taaaataacc tactataagt actactattg aacctaaaaa gcttaccata
    35401 aattcatccc cttagttttt atataattgc tttataaatt tttatgagtt taagaaaact
    35461 atttccagtt caaatagtaa tactttttta tgctaatata aatattaatt acattaagtg
    35521 catatttaag tacatgaaat gactttatat atgcattaac agcctaaatt cttttacttt
    35581 gcttctgctt acagttactt catagtcact attttttaat ttcaatatgt atgtattgtt
    35641 aaaccatggt ataatttctt ttactttggt taaatttacg atataagacc tatgtgttct
    35701 aaaaaatgtt ttatcattta taagattctc tacttctgat atgccttctt ttaatataaa
    35761 cttttctttc tcagtatata taaatgtctg cctttcatta gcttcacaat aatatatatc
    35821 atcaatatct attacaacca atttatcacc tttccaaagg ctaattttat gtgttatttc
    35881 ttctatctcc tgatttactg tctctttttt atatttatat aaattactat tgctattaga
    35941 cagttcaata tcactcatct cacttttttc taatttattc aacatagaaa taattctctc
    36001 ttcagaatat ggcttcagta cataatcaaa tacatctaaa gaaaaagcat ctactgcata
    36061 attttcatac gctgttataa aaacaatttt aggccttgat ttaaactgat ttaatgtttt
    36121 tgcaagaagc attccatcga gatgaggtat atttatatcc aaaaaaatta catctatttt
    36181 attttcctga ataaagtcta acacttctat accattagta aattcattta ctacctctat
    36241 gctactttta ttctcaataa aatatcttaa ttcctttctt gccggaaatt catcttccac
    36301 tattatagct ctcatacttc tactccttta cataaaatac tatcaaagtc ccattgtctg
    36361 ttcttcttat attaagccct cttccataca tcagtttaag ccttaaatgt acattataaa
    36421 gtccaatctt attttctggc atattatctt gatatacatt atttataatt tcctcactta
    36481 tacctatacc attatcctct attgatattc tcacagtatt ttctaatgaa taaggtaact
    36541 tttttatgga tattttcact acaccagctc taccactttc taaaatacca tgtattatcg
    36601 cattttccac taatggttgt ataatcaaac ttggaatctt tatatttaca tcttctatat
    36661 cataagatac agttagtaat tctccaaacc tagctttttc tatctctaca aaagatttaa
    36721 cttgttcaat ctctttattt atatcaatta agttatcact atattcaaga ttatatctta
    36781 gataagttga taaatttatt attaactctc tagccttatc tggactaaat cttataaatg
    36841 aagctattgt gtttagggaa ttaaacaaaa aatgcggatt aatttgtctt tgaagtgctt
    36901 ttatttctgc tttatttctc gcttctatat cttttttatc tttctcaatc ccctctacta
    36961 tagatataac aaatcctatt ccaatctgtc caacaattaa aggtaaatat atcccaacaa
    37021 ttatttgaat tgctaaagaa tgtggatatg atattaagta tataagtagt atagtaaaac
    37081 tttcagaaat catacctacc aatataccat acattactct atgttgcttt ggtatcctct
    37141 tatatagaaa tcctgataat acacctgcta gtatacttga taaaagacat ggaacagatg
    37201 ttatcccccc aatatccata aaatatctat gaatccctga aaaaactcct gctgtaatac
    37261 ctaccattgg accaaataaa atgccacctg atactataga tattattcta gtgtttacta
    37321 ttgaccccat ataatttata ccactataag ttccaaatat ggctaaaaaa gtaaaaacta
    37381 atgctataca gaccaagtca ttaaaactat attcttcctt ttgaaatatc tgcttaaata
    37441 tttttaactt tgttattaga agaaacagta ctacaatgag gcttgtcttt tctattaatg
    37501 cgattaaaat tgatgttttc atattccacc tttaaaaaat aaatttttac acactctaaa
    37561 tttattatgt ttgttgtaac ttatatattt gaagtaatcc tatctcgaca ttttccacat
    37621 ttaacctttt aattaaataa aaaatggata tacttctatt aacaactaat actagctgac
    37681 taacacaagc ttgtaataac ttgtagttat cattaactct acaattctag tatttaattt
    37741 tatactttag aaatctcttt tcttagaagt aatactttcg aatattatat aatacaatgt
    37801 tatgtagatt cttactcata caacatattt ctttaaaaat tttatactat tctttgttca
    37861 aatatctcat ttgatttaat tgtgctaaac acacattatc atctctctgt tatatattta
    37921 ttatatagcc gtatatcgat aaagtaaata aatcctatat ttaaaatcat atgtttgata
    37981 gtaaatcatt ataatgtata tttaccataa taatttgcca accttgaaag tcacttctat
    38041 aattagaagt taaataattc ttctaaaaaa aatacctcta aattttattt tctgaaatac
    38101 atagttataa ctatttttat cacgtataag aataaaaatt ccagaaaatt tttattatct
    38161 aagttaatat aaaacatatt atttaatata aataaatttt ttataataat atattgattt
    38221 aatttacttt attattgtaa actataagta taagatatac tatataaaca agcataaata
    38281 taccttaatt ctaactatcc ttaattttat ttatctccta gtgttatttt acgtattttt
    38341 tataaaatac ttttagtttt tttacttttg aattttttat aggagggtat ttagtggata
    38401 tattaatttt tgataatgat gtttgttttg gaataaaact aaaagaaaaa ataaataata
    38461 ttttaataaa agaaggtttt gataacgatg ttataagatt atattataat gcaaatttgt
    38521 tattaaaaga acttactgaa aaaaataaag taaaaatata ttttatagtt gtagatgcaa
    38581 aatacaagat aagtaatgaa ttatgtgatg gattatggat agcacaaaaa attagagaat
    38641 cagattatat tagtccaatc atatttctta caaatcatat agaaatgatt ttagggatct
    38701 tcgattatag gttagaagtt atggacttta tattaaaaca tgatatggaa attgctgaaa
    38761 gtaaaataaa agcttgtatt aaaattgctc ataaaagata cgttaaagaa aaaaattatc
    38821 gctctaattt ttttactatc tactcagatt cctcactatg gaaaatttca tttgatgaag
    38881 ttatatattt tgaaacaagc gctattccac ataaaataaa attagtgact acttcaagaa
    38941 tatttgaatt ctataaaagt ttaaggtctt tatcagattt agatgcatgc tttattcgtg
    39001 tacataaatc ttttgtagtt aataaatatc atattgtttc tctcgaccta aagaaaaata
    39061 atattaaaat gagtaatggc catatatgtc gtatatctaa tacatataga aatattttaa
    39121 agaacattat taaaacataa taaatagaaa aaagagatgt ctcaagataa gaatttgaac
    39181 catctctttt tttatacaaa aaaagtagtt cctaagaatc ctctatataa taatcgtttt
    39241 gtgaaaaaac acataatgac tatatcatat acaatttcta ttagacattg caaccattgt
    39301 tcctagatta tgattcaaca agatagctca ttaaatcata aaagtatgaa atagtctctt
    39361 taaaatattc agttgttatt ttgtactgac atatcatata aatacatatt tttatgatat
    39421 atagttacat attttatgaa atttatataa aaaattctta tttagattat ataatctaaa
    39481 taaattaaag ttcaagagtt aattaaacta atattgggag ggagaataaa tgaaaaaatt
    39541 taggaaacat aaaaggatta gtaattgtat atctatattg ttgatattat atctaacttt
    39601 aggtggtttg ttacctaata acatttatgc acaagactta caaagctata gtgaaaaagt
    39661 ttgcaatact acttacaagg ctcctataga aagaccagaa gattttctta aagataaaga
    39721 aaaggctaaa gaatgggaaa gaaaagaagc agaaagaata gagcaaaaac ttgaaagatc
    39781 tgaaaaagaa gcattagaat catataaaaa agattctgta gaaataagta aatattctca
    39841 gacaagaaat tatttttatg attatcaaat agaagcaaat tctcgagaaa aagaatataa
    39901 agaacttcga aatgctatat caaaaaataa aatagataaa cctatgtatg tctattattt
    39961 tgaatctcca gaaaaatttg catttaataa agtaataaga acagaaaatc aaaacgaaat
    40021 ttcattagaa aaatttaatg agtttaaaga aactatacaa aacaaattat ttaagcaaga
    40081 tggatttaaa gatatttctt tatatgaacc tggaaaaggt gatgaaaaac ctacaccatt
    40141 acttatgcac ttaaaattac ctagaaatac tggtatgtta ccatatacaa atactaacaa
    40201 tgtaagtaca ttaatagagc aaggatatag tataaaaata gataaaattg ttcgtatagt
    40261 tatagatggg aagcactata ttaaagcaga agcatctgtt gtaagtagtc ttgattttaa
    40321 agatgatgta agtaaggggg attcttgggg taaagcaaat tataatgatt ggagtaataa
    40381 attaacacct aatgaacttg ctgatgtaaa tgattatatg cgtggaggat atactgcaat
    40441 taataattat ttaatatcaa atggtccagt aaataatcct aacccagaat tagattctaa
    40501 aatcacaaac attgaaaatg cattaaaacg tgaacctatt ccaactaatt taactgtata
    40561 tagaagatct ggtcctcaag aatttggttt aactcttact tcccctgaat atgattttaa
    40621 caaactagaa aatatagatg cttttaaatc aaaatgggaa ggacaagcac tgtcttatcc
    40681 aaactttatt agtactagta ttggtagtgt gaatatgagt gcatttgcta aaagaaaaat
    40741 agtactacgt ataactatac ctaaaggttc tcctggagct tatctatcag ctattccagg
    40801 ttatgcaggt gaatatgaag tgcttttaaa tcatggaagc aaatttaaaa tcaataaaat
    40861 tgattcttac aaagatggta ctataacaaa attaattgtt gatgcaacat tgatacctta
    40921 atattttttc acataaataa tttaatattt ttcaaattta aggaggagaa acaatgaaaa
    40981 tacaaatgag gaataaaaag gtattaagtt ttttaacact tacagctata gttagtcaag
    41041 cactagtata tcctgtatat gctcaaacta gtacaagtaa tcattctaat aagaaaaaag
    41101 aaattgtaaa tgaagatata ctcccaaaca atggattaat gggatattat ttcacagatg
    41161 agcactttaa agatttaaaa ttaatggcac ccataaaaga tggtaattta aaatttgaag
    41221 aaaagaaagt agataaactt ctggataaag acaaatcaga tgtaaaatct atacgatgga
    41281 caggaagaat aattccttct aaggatggtg aatatacatt atcaactgat agagatgatg
    41341 tcttaatgca agtaaatact gagagtacta tatcaaatac acttaaagtt aatatgaaaa
    41401 agggtaaaga atataaagtt agaatagagc tacaagataa aaatttaggt tcaatagata
    41461 atttatcatc acctaatctt tattgggaat tagatggtat gaagaaaatt ataccagaag
    41521 aaaatttatt cttaagagat tattctaata tagaaaaaga tgatccattt atcccaaata
    41581 acaatttctt tgacccaaag ttgatgtctg attgggaaga cgaagatttg gatacagata
    41641 atgataatat accagattca tatgaacgaa atggatatac tattaaggac ttaattgcag
    41701 ttaagtggga agatagtttt gcagaacaag gctataagaa atatgtatca aattatttag
    41761 agtcaaatac tgctggagat ccatatacag attatgaaaa agcttcaggt tcttttgaca
    41821 aggctataaa gactgaagca agagatccgt tagttgcagc atatccaatt gttggagtag
    41881 gtatggaaaa attaattata tctacaaatg aacatgcctc tactgatcaa ggtaaaactg
    41941 tttccagagc tactactaac agtaaaactg aatctaatac agctggtgtg tctgttaatg
    42001 taggatatca aaatggattc acagctaatg taactacaaa ttattcccat acaacagata
    42061 attcaactgc tgttcaagat agtaatggag aatcatggaa tactggatta agtataaaca
    42121 aaggagaatc tgcatatata aatgcaaatg ttagatatta caacacaggt actgcaccta
    42181 tgtacaaagt gacaccaaca acaaatttag tgttagatgg agatacatta tcaactatca
    42241 aagcacaaga aaatcaaatt ggcaataatc tatctcctgg agatacttat cccaaaaaag
    42301 ggctttcacc tctagctctt aacacaatgg atcaatttag ctctagactg attcctataa
    42361 attatgatca attaaaaaaa ttagatgctg gaaagcaaat taaattagaa acaacacaag
    42421 taagtggaaa ttttggtaca aaaaatagtt ctggacaaat agtaacagaa ggaaatagtt
    42481 ggtcagacta tataagtcaa attgacagta tttctgcatc tattatatta gatacagaga
    42541 atgaatctta cgaaagaaga gttactgcta aaaatttaca ggatccagaa gataaaacac
    42601 ctgaacttac aattggagaa gcaattgaaa aagcttttgg cgctactaaa aaagatggtt
    42661 tgttatattt taatgatata ccaatagatg aaagttgtgt tgaactcata tttgatgata
    42721 atacagccaa taagattaaa gatagtttaa aaactttgtc tgataaaaag atatataatg
    42781 ttaaacttga aagaggaatg aatatactta taaaaacacc aacttacttt actaattttg
    42841 atgattataa taattaccct agtacatgga gtaatgtcaa tactacgaat caagatggtt
    42901 tacaaggctc agcaaataaa ttaaatggtg agacgaagat taaaatccct atgtctgagc
    42961 taaaacctta taaacgttat gtttttagtg gatattcaaa ggatccttta acatctaatt
    43021 caataattgt aaagataaaa gcaaaagaag agaaaacgga ttatttggta ccagaacaag
    43081 gatatacaaa atttagttat gaatttgaaa ctactgaaaa agattcttct aatatagaga
    43141 taacattaat tggtagtggt acaacatact tagataactt atctattaca gagctaaata
    43201 gtactcctga aatacttgat gaaccagaag ttaaaattcc aactgaccaa gaaataatgg
    43261 atgcacataa aatatatttt gcagatttaa attttaatcc aagtacagga aatacttata
    43321 taaatggtat gtattttgca ccaacacaaa ctaataaaga agctctcgat tatatccaaa
    43381 aatatagagt tgaagctact ttacaatatt ctggatttaa agatattgga actaaagata
    43441 aagaaatgcg taattattta ggagatccaa atcagcctaa aactaattat gttaatctta
    43501 ggagttattt tacaggtgga gaaaatatta tgacatacaa gaaattaaga atatatgcaa
    43561 ttactccaga cgatagagag ttattagttc ttagtgttga ttagtatcaa atgatttaaa
    43621 tttgtcccca caatacaata tatatttacc ttataaagta ctaaaaaatt aactttataa
    43681 ggtatttttt atttaaatat tctcttcttc taacatattc ttttttagaa ctatattgta
    43741 ttataaagat aattatctgt atcatatcac ctatatataa acataaattc atttaattat
    43801 ataaatttaa aaaatatttt tttgatatat tattgacaat ttccattata agagttaaaa
    43861 ttaactcata aattacatat tgggtataaa atacaatgta tgttagaagt tgtttcagaa
    43921 ggctatggtt ttctgatttt tttatgctca gtagtgatat gcaaaaaaga caaagttgac
    43981 atcagttaac ttttaccttt attctacttt ccccaaagta agaataatct aacccctttg
    44041 agatgagcaa aagctcatct ttttttatta tcaaaagatg ctgtctcaaa atagagataa
    44101 tcctcttttt cattgttgaa gtgattaaaa tatattttat ttcatacaaa aaagagtgtc
    44161 tcatgaacaa aagttcactt tgagactctc tctttcatat catatacctt tttaataact
    44221 ttaaaattta tttatcaagt aaagatagct aataattagt atcttctctc tataaatcca
    44281 acttttttat aaacttttct aagagttttt ctagcttgtt tttcggcttt ttctgctccc
    44341 atactatata ttttttctaa ataatcttta ttattaagaa ggtcaacata tttttctctt
    44401 ataggtctta aagcttctga tactacttca gccacatctt ctttaaatat gccataaccc
    44461 tttccttcat acatgtttac aatctcttct acactctttt tacttaaatt agaatatata
    44521 tcaattagat ttttaatacc tggttgttca tcattgtatt ttacaacacc taaagaatca
    44581 gtaacacttc tttttatctt tctctttata gaatctgaat catctgctaa tagtataaat
    44641 gcattttcat ttgaatcgga tttactcatt tttttagttg gctcttgcaa actcatcact
    44701 cttgctccac cttttgggta gtatccttct ggaactacaa aagtaggtga aaatctatta
    44761 ttaaacctat ttgctaaatc acgagctaat tctaaatgtt gtttttggtc atctccaact
    44821 ggaactaggt cagtttgata caacaatatg tcagcggcca taagaactgg atatgtgaat
    44881 aaacctgcat ttagatttgc ttcactcttt tgtgatttat ctttgaattg agtcattcta
    44941 cttaattcac ccatataagt catagtattc aatacccaca taagttcaac atgctctttg
    45001 acatgtgatt gtataaatat agtatttttt tctgggtcta atccacaagc tatatattga
    45061 gcaagtaatt ctatagcatt cgctcttaaa actttagggt cctgaggtac tgttattgca
    45121 tgtaaatcaa ctatactata atagcaatca taattatctt gaagttcagt ccagtttttt
    45181 atagctccta aataatttcc taaagtcatt ttgcctgatg gctgtgctcc actaaatata
    45241 actttttttt cactcatttt tttgtacctc cttatatatt tatattgatt ttaacatatt
    45301 taaaactatg tctttagagt tattggcagc atcatgtaca aatacatcat aagtaacatc
    45361 agcacttcca tctgccttat cagacattgc tcttattatt acgaatggag tattatttaa
    45421 atagcataca tgagctatag ctccaccttc catttctcca caatatccac caaaatcatt
    45481 tactaattct tcttttaatt ctttagaatt tataaattta tctcctgtaa ctactcttcc
    45541 ttttaatact ttatgagtct ttacttcttc cacagaagat ttgtaagcag catctataag
    45601 tctttcatct gctttaaata cagaagtctt cattcttggt ataactccct tagggtctcc
    45661 aaatgctgta gtatctacat catactgtat agcatcagtt gatattacta tatcattaac
    45721 atcaagtttt tcatttaaag ctcctgcaac acctgtatta acaatagcat caaccttaaa
    45781 ttcgcttatt agtatctggg cacaaagagc agagttaacc ttacctatac cacactttac
    45841 taacactaca ttcttacctt tcaaaattcc cttataaaat tctaagctag ctttttttat
    45901 agtctctcta atatccatta aatcaactaa aatactaact tcttcatcca ttgcacctat
    45961 aattcctatt acgttcattt ttcctcctaa attttctata ttaaaaaatc cgtcctaaat
    46021 caataggacg gatatatttt ccgtggtacc acctaaattt atatacactc aaaactgaat
    46081 atatatatct taatatatgc tataacgtgc atacacgata tcaaatactc tgtaatctta
    46141 aattacattt cattttactc ctcaaagacc cattcaataa atacttttta ttaagttctc
    46201 agcatcctta attctctgtg caaaaatata tttatttact atttcttatc tcaggattta
    46261 atttattaat ttctttattt tataatattc cttacactat aaattgtcaa ctatatatta
    46321 taatatccaa aagaaatttt taaaatacat tttatttagc tgttttcttt tcatatctat
    46381 tccataacca tagaccagat aatataacta gaagacctcc accaatcatc tctatctcat
    46441 atataacatt ttcagcaaat gtcggcataa ctggtgctgc agataataat ataccaacta
    46501 cagttacaac taaaaccatt tttgctaaac ctaggcaagt acttgttgat tttgccatag
    46561 tataaggtct aatttcatct ggtctctctt ttcttagttt aatataagaa gcatataata
    46621 atacatatgg gaataatgca gttaatgctg tcatagttac aagaacattg tatatagcat
    46681 caacagaagg cattaatgta gtcaccaata gtattatagt aactattatt gcttgaagta
    46741 taactgcttt agaaggtatc ttgtgttcat ttacctctac caaactatct ggaaatattc
    46801 cttttttaac actaccaaat aacattttta ttggtgatgc tatataaaga actattgctc
    46861 ctaatactga gaaagctata ccaaaagcta ttaattgaac caaccaagaa ccaattccta
    46921 aagacgtaca aacagcagaa agtgcatcta atacaccttt agaagcagta atttggtctg
    46981 gtggtattat cattgttatt gcaattgaac ctaatacata taatgaagct actattccag
    47041 ctgatattaa tattgcctta gggaagttct tttcaggctt atctatttct gttatgaagt
    47101 tagcagcagt ctctgctcct gctaaaccaa acattatagc tgatatagca acaaatgcat
    47161 ccatattaag ttttggagtc attgaagcta ttgtatatgt agaagctata tcatgttttt
    47221 tcaacataaa tactgctaca aatgaaaata ttataagtat agcacttggt attgttgaac
    47281 ctaatgcacc tgtattagta aatatttttg taaaacttat tcctttagtt gctataagag
    47341 ataatgccca gaatattatt aaagataata taagtacaaa catcttatta tcagcaagag
    47401 ctggatttcc tattgtataa gaaacattta ctgctaagaa agttaaaaat gaagaatacc
    47461 aaaatatttt agctgtccag tttaaccaag aaactaaaaa tccatacttt tctccaaagg
    47521 cttccttaac ccaatcgtaa agacctccat cacccttttg atatgtagaa gctaattcag
    47581 cgcaaattaa agctcctggt acaaagtata taaatgagaa taataaccac ataggtattg
    47641 cacctaatcc taaaccaaag cttgatgctg tagacttagc tatccatctt ataccatata
    47701 aagctgatac attcataaat accaaactcc ataggcctat ttttttcttt tccatcctaa
    47761 tttcatttcc cctctctatt aattatttta aatttaattt caaaattaat aaaatagatt
    47821 cattattatt aaactgatat ttttcaaaat atttgcatat tcaaaaacag ttttaataat
    47881 aaaccttaaa tactttgaaa tcaaatctta ttaaaaatga taaatttaaa agttttacca
    47941 ctttaattct aatgatacta aatttttaaa attgtttcaa tattatttta ttataaaatt
    48001 ttatttatta tttttcatta gtatataaaa agataaaata cataacttta tagacttaat
    48061 ttattacttt tatagaattt tcacaataaa aagtcatttt acttccgtat ttaatgcaaa
    48121 agtagagatt aaacttaata taaaaaatat ataacaaact ttttttatat ttctagaaga
    48181 aaagtatact cttttttgtt taataatgct aaaattgatt tgtattgaat tttgattagg
    48241 aggaaattat tatgaaagct caaagattaa atgctgtact tgaacaaatg aaaaaagatg
    48301 atatttctca aatgttagta agtgacccta cttctatatt ttatcttaca ggagtactaa
    48361 tccatccagg tgaaagatta ttagcattat acttaaattt aaatggcaat aataaattat
    48421 ttataaatga attattccca gtttcagaag atttaggtgt tgaaatggtt tggtttaatg
    48481 atactcaaaa tccagtagaa ataataactg aacatataga caaaaatgct actatgggtg
    48541 ttgataaaaa ctggccagct agattcttat taaacttaat agaattaggt ggaggaagta
    48601 agtttgtaaa ttcatcttat ataatagata ctttaagaat gtgtaaagat gaagaagaaa
    48661 aagagttaat gagaatagct tcaaaattaa atgacaaggc tatggaacaa cttaaagcta
    48721 ctgtttctgg tgaacttaca gagaaacaac ttgttggtaa attatctaaa atatatgaag
    48781 acctaggtac tgatggattt tcatttgacc ctattatagg ttttggacct aatggagcta
    48841 atccacatgg tgaacctgga aatgcacttg tgaaaccagg agatgcaata atattagata
    48901 taggttgtat aaaagataac tattgtgctg atatgacaag aactgtattc tataaagaaa
    48961 tacctgaaaa aggtagagaa atatttgaaa tagttcttga agctaataag agagctgaag
    49021 ctattgtaaa accaggtgtt agattctgtg atatagatgc tgctgctcgt gactacataa
    49081 ctgaaaaggg atatggacag tacttcactc atagaactgg tcactctata ggacttgaag
    49141 ttcatgataa aggagatgta tcttctataa atacagatac tgtacaacca ggaatgatat
    49201 tctctataga gccaggtata tatttaccag gtgagttcgg agttagaata gaagatttag
    49261 tacttgttac tgaagatggt tgtgagatat taaacaaaca tgacaaagaa atatgtgtag
    49321 ttggttaatt ctaaaatcaa tttgataaaa ttaaaaagcc atataaaaat gttttaaaca
    49381 tttttatatg gctttaattt ctcaatctat gatttatttc aatccattat ttatattgca
    49441 gttattaaga ttgcaccctt tgaagtatat gtccccatca ctgaaccaat ttcacagatt
    49501 acaacatctt tgtattttac atccttttcc ataacttccc ttaccttttg agccttttct
    49561 gggcaattag catgtcctat aaataaaacc ttttcttctt tatctttaat ttttgactct
    49621 actaaagtaa tcagcttttt caaactgttt ccttcacctc ttgccttatc tataggagtt
    49681 acaacgccat ctgcaacttg tattatcgcc ttaaaattca aggtatttat aatttttcct
    49741 gctattggat ttattctacc acccttaata gcattttcta aagtttcaag tgttccaaaa
    49801 aatggaggat tatctttata tttatttatt attgaaacaa tctcttcaat ttcttttcct
    49861 tcttttatta atattgctgc ttttaacact aattgtccta ttccaattga cccactaagt
    49921 gaatctataa cctcaacttt tttgttattt ccttcctttt caaacatatc ttttgctaaa
    49981 agtgcactat tataagtccc agataatcct gaacttatat taatcaccaa tacactttct
    50041 tcctgacaat ggtatgcttc taaaaattta tcaggagaag gacatgatgt ttttggtagt
    50101 actttactct cactcatttt tctatagaaa gtttcattat ctatttcctt tccagatata
    50161 taactttctt ctccaaaaga tacatttaaa ccaacaactt ctatgttgta tttttctatt
    50221 atatcatcag gtaagtcaca tgcactgtcc actatcaatt ttattttatc catattaacc
    50281 tcactataag atattatttt atttattcag tcggtcgtac gaccgattta attattattt
    50341 tatatattga atgaaaaaaa gtcaatagat atagattctc gctttattga caataaatat
    50401 tcataagctt ataatgttat taatttgtta taaatataca tggaaaggta ggttttatga
    50461 aaacaaataa tatgaaacag gctattttat catctgcaac caatttaata gccaaaaatg
    50521 gagttcaaaa tactagcctt gctgatattg caaaagatgt aaatataagt aaaggtactt
    50581 tgtattatca ctacgcatct aaagatgata ttatatatga tattgcggat actcatttag
    50641 aagttataac tagtgctata ctaaactgtg taaaaaatgt caaatcaaaa aattctcaaa
    50701 tagaaatggt taacttaatc ttagagaaaa tatctactat agaaagtcgt ggtagggtac
    50761 atatgtatct tatttgtgag gcaattactt caaataatga tttaaaggaa agaataagat
    50821 taaaatatat tgaatggaga actactctta aatctgaaat tgttgaatca ctagaaaaat
    50881 ataatagtag cgaaaatgaa caggatgctg aatcattttc ttttttactc atgtcaatag
    50941 ttgatggttt agttgtccaa agtttattaa aaacagaaaa aataccttat gaaaatatag
    51001 cttctttttt agttaatcat tggatttaaa cttaatactt agataattaa taaaagtgaa
    51061 ctatttaagt tatctacttt aacagttcac tttcttattt attctcaata atttttactt
    51121 aaaacattca atttttattt aaaccttcat ggaatcacaa aataaaaatc tcttataaat
    51181 ttaaaatgtt tttatttatg ttcatgtgta tgcccatctg aatgttcatg accatgtgta
    51241 tgtgtatgct catggctatg ctcatgagta cctgaaaaac catcatcatg attatggtcg
    51301 tgacctacac catttacatg ctcatgttca tgcccatgac tgtgagaatg ttgatgtgaa
    51361 tgttcatggt tatctcctcc acagcatcct tctcctccag tcccacaaca cccacttgta
    51421 tgtggattat gatgatttct tactacaaat ctctctctta acataattct tatacctcct
    51481 agtatattat tgaatacttc atgtacccca ttaactacag tatacatcta actaggtata
    51541 atctcaatac atatggcatt ttttaacata tataattaac atatataatc ttaaagaaat
    51601 ttatccttaa aacctccacc ttttacttct tcaacgttat tgacagaaat aaaagcttca
    51661 ctatccatat cataaattaa atctttaaat ttacctattt catttgcaga tattatgcaa
    51721 taaatcatct ttttcttatt ttgtgtatat gcaccttctg cctccaaaaa agtgactcct
    51781 ctacccattt tattcattat cactttggat atttcatcat atttatttga tattaaaagt
    51841 atagattttt ttctatcaaa gcaatctttt cctatatcca tagtaaaaga aattatatac
    51901 attgtaatta aagtgtacat caccaattta gctccaaata agaaagctcc tgcacatact
    51961 acaaaaaaat taataaataa aaatgtattt ttcataggta tatcatactt tcttttcatt
    52021 atggctgcta ttatatcaag tcctccagca gtagctcttg ctttaaaagt taaccccata
    52081 cctataccag ttaatgcacc accataaata cactgtagca tagtatcatc tattgggaaa
    52141 tatttagcaa tatcttgtgt tagccccagt gctagagaaa acagtaacat attgacaaaa
    52201 cttattagca aaaatttatt atcaaaatac tttctagcta taataaatat tggtatgttc
    52261 atcaagaaac ttgatattcc ctgatttata ccaaataatt tgtataatat aacacaaatt
    52321 ccagctactc ctccacttaa taacttggct ggtgctagat ataaatttat tcctaaggtt
    52381 gatagaatca tacccaatat aatcagacca tattgtaaaa atatgttatt ctgaacctcc
    52441 catgactaaa gtcacagggt tcctgcttca tagaattttg catactaaaa tatgtcttac
    52501 taattctcca caagctatcc ccgtagttcc tacggttctt acataagtta tttattttga
    52561 attgttatta gtcttaatcc ttcttttaat atatttat
//
LOCUS       DAELXX010000013        50501 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.013, whole genome shotgun sequence.
ACCESSION   DAELXX010000013 DAELXX010000000
VERSION     DAELXX010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 50501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50501
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.013"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..2604
                     /locus_tag="KPH29_000763"
     CDS             <1..2604
                     /locus_tag="KPH29_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG1466393.1"
                     /translation="IIINEKEKFKVLTDNILGGVKTCLKDEYMTMTYVSQGFLELTGY
                     SCEDLNKIFKNRFKDMIYKDDLDKFNCIILNQLEKQDKIEVEYRIVRKDGSIIWILER
                     SKSINDNKGSECIQGVLTDITELKKIQEELDSKRFEIQAINDSIVGGIVITEINDDFD
                     SIYLNEGYLNMIGYTRKQLEDELNNKLIKLIFKDDRTELINSINKQLKLSDTFASEQR
                     VEKRDGTIIWILLKGRKIIYDNGKSVLCCVIVDITSAKLLEQELKKSEERHEIILKQT
                     QNIIIEWDIEKDKMNISPNWYNKFKYDPITENATKNIIKSVDIYHDDMDKFISLIKLT
                     SQGSEYEECEIRLRTRDTIYAWFSIKATTLFDNNGKPYKVVGSLTDIDESKREAEKLK
                     ERAEKDPLTDLYNKKASQSLIEEYITLDKKRKGALMIIDIDNFKGINDNLGHLYGDAV
                     LSEIANDLINLFRATDIVGRIGGDEFIVFIKDISETRDIINKAEELVKTFERSFLGKG
                     RNYKISLSVGIAKFPQDGMDYMTLFRNADMALYSAKGKGKNCYTLYDKKIDKVQYITN
                     TRNAGDSDNVEMSFKEHITNYIFDILYESSDIEVAVNLILSIVGKHFNVSRMYIFENS
                     DDNRVCKNTFEWCNEGVTSEINNLKNICYKDLGNYLLNFNADGLFYCNDIENLGSDVK
                     KVLKPQGIRSILQCKILEGNEFRGFIGFDECSYNRMWTNDEVNTLTFISKTLSMFLLK
                     MRIQSKLEESLTIQKSVMDNMDIWAYVVDRNTYELLFINKKMLESASNTHVGDLCYKA
                     IWNNQDKPCKKCPMIYLKDGDDTYKSFMRNKKFEVQANITTTTLKWFDGKDACLIFGS
                     EVTSIEAEENT"
     gene            2789..4723
                     /locus_tag="KPH29_000764"
     CDS             2789..4723
                     /locus_tag="KPH29_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBG1466394.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLENAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            4829..5824
                     /locus_tag="KPH29_000765"
     CDS             4829..5824
                     /locus_tag="KPH29_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG1466395.1"
                     /translation="MKKYISIILLVVVTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSNIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            5796..6581
                     /locus_tag="KPH29_000766"
     CDS             5796..6581
                     /locus_tag="KPH29_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG1466396.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIMLNSSFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRN
                     LLKPINALTRSIPTMILVVLALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNL
                     LEMANVYKIRFIDKVLKIYLPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            6569..7267
                     /locus_tag="KPH29_000767"
     CDS             6569..7267
                     /locus_tag="KPH29_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1466397.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            7390..7863
                     /gene="ybaK"
                     /locus_tag="KPH29_000768"
     CDS             7390..7863
                     /gene="ybaK"
                     /locus_tag="KPH29_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBG1466398.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKLYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            8060..8899
                     /locus_tag="KPH29_000769"
     CDS             8060..8899
                     /locus_tag="KPH29_000769"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HBG1466399.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKK
                     VVI"
     gene            8912..9460
                     /locus_tag="KPH29_000770"
     CDS             8912..9460
                     /locus_tag="KPH29_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HBG1466400.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            9526..10707
                     /locus_tag="KPH29_000771"
     CDS             9526..10707
                     /locus_tag="KPH29_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HBG1466401.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEVKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYIYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            10880..11143
                     /locus_tag="KPH29_000772"
     CDS             10880..11143
                     /locus_tag="KPH29_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG1466402.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            11669..12352
                     /locus_tag="KPH29_000773"
     CDS             11669..12352
                     /locus_tag="KPH29_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1466403.1"
                     /translation="MSSILLVEDDLSLIYGLEFSIQKSGFSIDIARTVKEAMQMYEEK
                     NYDLLLLDVSLPDGNGFQICEKVREVSNIPIIFLTASDEEVNVVMGLDMGGDDYITKP
                     FKLNELISRIKALLRRYNVASNVNELSSNNITIKLLENRVFKGEFEVELTTAEYKLLC
                     LFMKNKNIVLTRKNILDKLWDGNGSFIDDNTLSVYVRRLRNKIEDNPENPKFLLTVRR
                     MGYKWNVIK"
     gene            12363..13604
                     /locus_tag="KPH29_000774"
     CDS             12363..13604
                     /locus_tag="KPH29_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1466404.1"
                     /translation="MKIFSNKDIKIFFLILISILVMFIIVGQIVVISITNNYKKTLIE
                     HDYNIAGYLYSIGVDKSKIPSAFVAHEDKYILKGKEILDTAGYGFDIDVSFTPSVKLF
                     QSKYIKTMSIYLLVFFTMIIFILYMYFFRQQSKIEEASYKIDLFMNGNINERLNTYEE
                     GSLSKLFSSIDVMSTSLNTHILKEKQSREFLKNTISDISHQLKTPLTALIMYNQILQE
                     ESHNSDVVEAFVQKSQSELERIETLIKNLLKITKIDSKTIVLNKNNVNLKNLIVKILY
                     SFETRAEKEGKVISINGSENTLLFCDYEWMSEALSNLIKNALDNTKENDKITIEWFET
                     TITTIVSVKDTGNGIHVEDIHHIFKRFYRSKFSKDNQGLGLGLPLVKSIVEQHNGTIS
                     VESKLSQGSTFTISFLKLTNM"
     gene            13676..14359
                     /locus_tag="KPH29_000775"
     CDS             13676..14359
                     /locus_tag="KPH29_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1466405.1"
                     /translation="MDILVVNNLCKTYGNGYTKVEALKDVSFTVSKGEFVTVVGESGS
                     GKSTLLNLLGGLDTPTSGRIFIDGKEICSMKEEKQTIFRRRNIGFIFQAYNLIPELTV
                     EQNIIFPLLLDYKKPDTIYLDEVLTILGLKDRKNHLPNQLSGGQQQRVAIGRALITKP
                     SIILADEPTGNLDSKNSNDVISLLKVTAEKYNQTIIMITHNTNIASTADRILQVSDGV
                     LTDLGVNRE"
     gene            14356..16686
                     /locus_tag="KPH29_000776"
     CDS             14356..16686
                     /locus_tag="KPH29_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG1466406.1"
                     /translation="MRSYLELVPQYAKVHKKKNRTTILCIIIAVCLVTAIFGLADMAI
                     QCQKIQFIKDDGNWHIVIRNIDEKFISEINSRVDIAHHGRGQVVDSGEFKSKEILIQG
                     LDEEVAKQFNLEIIRGRYPIGENETLIDEDAVKSFNLKIGDFIKIKLADGRNKNYKIV
                     GEYSSISSLRATDKHGLILSILGTQAIKKSETYDYYYITFKEKVNIRKATSDIKNTFN
                     LSDKQIGKNERLLSIIGQGDSDFSKPLYLTAGLLFVLVLVASIIMIYNSFNMSVIERV
                     KFFGLLRCLGSSKHQVKKFVVLESLILSIKAIPIGLVLGSLVTIISSIFLKYVNELFS
                     SMPILKVSFIGIAFGIIVGFLTVILSAIIPAKKASRVSPLSAVRGNLMSKEILNNGKT
                     SKVANTGSFKIETSMGLNHAFSRKKSFVLMTSSFAISIILFLGFSVLIDLMYQGLKPM
                     KPEAPHISIVDPNFKSVLTKEDINEISNIKGVKKVYSRMMTDIEATYNGKKTKSQLIS
                     YEKYQFNWAKKYLLKGEVNENKLQSENLALVEEGHGFDIGDKIYIKNRNAEKELEVGG
                     ILSSIPFEAKDKYIGNIITSENTFTNITGIKGYIIMDIQVDKDTPGDLIENIRRVVDD
                     NLEVRDKRQSNLEVKNSFYTMATFVYGFVFIIAIISMFNIINSMNISVTSRKNYYGVM
                     RAVGISNRQLRRMVIVEAGSYAMSGCLLGSILGLMLHRYMFISLVSSMFHLEWQVPFN
                     LLLITVLTMIIITLLSVRKPVKKICELDIIDSINAQ"
     gene            complement(16867..17709)
                     /locus_tag="KPH29_000777"
     CDS             complement(16867..17709)
                     /locus_tag="KPH29_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG1466407.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKAGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            18004..18738
                     /locus_tag="KPH29_000778"
     CDS             18004..18738
                     /locus_tag="KPH29_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG1466408.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGSPIEYTISIFRTDKYQYEIVLSE"
     gene            18748..19881
                     /gene="nagA"
                     /locus_tag="KPH29_000779"
     CDS             18748..19881
                     /gene="nagA"
                     /locus_tag="KPH29_000779"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBG1466409.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNITHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLDNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGDVVYKNI"
     gene            19980..20729
                     /gene="nagB"
                     /locus_tag="KPH29_000780"
     CDS             19980..20729
                     /gene="nagB"
                     /locus_tag="KPH29_000780"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBG1466410.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKLENTHLP
                     NGMADDIEKECLNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            20919..21176
                     /locus_tag="KPH29_000781"
     CDS             20919..21176
                     /locus_tag="KPH29_000781"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466411.1"
                     /translation="MFSGILSSIIKLPIIGILPIGNIKKNTGISDSKKDGKQRRSNLG
                     ISIYQLPSFLYSLINNSVPSNKPIPPEEIKLIKMYLLKSCI"
     gene            21836..22540
                     /locus_tag="KPH29_000782"
     CDS             21836..22540
                     /locus_tag="KPH29_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1466412.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAVSRDELLNKIWGYDIEVETRAADDTVKRLRKKILDTNI
                     LIETVWGFGFRLKEKS"
     gene            22542..24029
                     /locus_tag="KPH29_000783"
     CDS             22542..24029
                     /locus_tag="KPH29_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1466413.1"
                     /translation="MNNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTEIVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDENTRISTRNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVFSNCGARQNVRAINKGIKFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
     gene            24234..25847
                     /locus_tag="KPH29_000784"
     CDS             24234..25847
                     /locus_tag="KPH29_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG1466414.1"
                     /translation="MKEQLIFNITNLVTVVFEAFVIHMFLSDFLGQKEGYIKVVRYAK
                     LGFIICLGFCNLITLNPKITMPLIFILIFSTSFLYKGNLKTRLFTTVLLSIFFILSEI
                     VVTSIFVLFVKEGFEIMLENNSIRVLATILSKIVFLLTCKIICLFKKDVHLDMPIKYW
                     LPLFLIPIFSLFLSVSIFDVSKFFSLESLKFLSLISSVGILYINFIVFYLFKFIIDKT
                     KLSMKYEILEKEIIHKEELRLSNECYKIIVEQTDSVVFEWNIKENKSFVSQAWTEKFG
                     YNNACKNIFKEIKDKDLVHSEDKAIFEGFLESIKKKNIHSQAVYRLKKSNGEYIWCRT
                     SITSIYNDENELLRVVGVIVDVDSDIKKYEELRTRAESDSLTNIYNKGTFEKLVEETI
                     VMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQASFGNKDLVGRIGGD
                     EFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDSSVSIGIARYPQDGTSFFEL
                     FKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(26537..27367)
                     /locus_tag="KPH29_000785"
     CDS             complement(26537..27367)
                     /locus_tag="KPH29_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG1466415.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKNACNIDANTITLKEHEEEYLVKTPITYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIKPKGLYVTA
                     YHKGSYETIYKAYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKI
                     "
     gene            27440..29167
                     /locus_tag="KPH29_000786"
     CDS             27440..29167
                     /locus_tag="KPH29_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1466416.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLIMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFVKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMDNMNRVLREVVTGVRVIRAFNREHFEKKRF
                     DRTAIDYSETAISINKVFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALI
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGRKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDVKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEIKHAASIAQADE
                     FISDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            29204..31066
                     /locus_tag="KPH29_000787"
     CDS             29204..31066
                     /locus_tag="KPH29_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1466417.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKVKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRRAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFSSEYDEEVV"
     gene            31399..32088
                     /locus_tag="KPH29_000788"
     CDS             31399..32088
                     /locus_tag="KPH29_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HBG1466418.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYPIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNNCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSIDLFNKDLPTVSVIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            32193..32879
                     /locus_tag="KPH29_000789"
     CDS             32193..32879
                     /locus_tag="KPH29_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HBG1466419.1"
                     /translation="MILIMTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMV
                     YKKTYMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDP
                     MDLVFLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNED
                     SEDIATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKYGETSLINDLSQVDGVTNAV
                     LVSYNGDYVA"
     gene            32896..34803
                     /locus_tag="KPH29_000790"
     CDS             32896..34803
                     /locus_tag="KPH29_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HBG1466420.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKSVDLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLTSVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETHKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKRSGNQSDKKEVNAELTSSVVKT
                     NTDNETENKTTNDSESKNNTDKDKSGNDNNQKLEGPRGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPREAGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
     gene            35256..35945
                     /locus_tag="KPH29_000791"
     CDS             35256..35945
                     /locus_tag="KPH29_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466421.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADS
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
     gene            36381..36920
                     /locus_tag="KPH29_000792"
     CDS             36381..36920
                     /locus_tag="KPH29_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1466422.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            36939..37982
                     /locus_tag="KPH29_000793"
     CDS             36939..37982
                     /locus_tag="KPH29_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1466423.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            37984..38820
                     /locus_tag="KPH29_000794"
     CDS             37984..38820
                     /locus_tag="KPH29_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1466424.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            38925..39599
                     /locus_tag="KPH29_000795"
     CDS             38925..39599
                     /locus_tag="KPH29_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1466425.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGIK"
     gene            39599..40651
                     /locus_tag="KPH29_000796"
     CDS             39599..40651
                     /locus_tag="KPH29_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG1466426.1"
                     /translation="MIKFKKTISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            41095..41943
                     /locus_tag="KPH29_000797"
     CDS             41095..41943
                     /locus_tag="KPH29_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG1466427.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIATPVSSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEE
                     ELQYDV"
     gene            41933..43597
                     /locus_tag="KPH29_000798"
     CDS             41933..43597
                     /locus_tag="KPH29_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG1466428.1"
                     /translation="MMFKIFNKSLLKLVIVLSIVLTISNFSLFSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINENYYSHIFILGINENS
                     YNNDKNTKNLISSLNSYKVTICWLGYGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYMGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLDKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDKDLDE
                     ITKSDTRFKYISNISKILIFIVSISVLIFVIIFIYFKRIDKKKFLK"
     gene            43646..44902
                     /locus_tag="KPH29_000799"
     CDS             43646..44902
                     /locus_tag="KPH29_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG1466429.1"
                     /translation="MRDLNVSEYLFVFSLFSIWSLLLINIILAMGGYIFYFKNFDKEI
                     KEIDEYPMISILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILEN
                     IKDRYNNYNFTIINTDSLTGGKGKSNALNIGYKVSKGDFIAVYDADNTPDKNALRYLV
                     QTIVMNDELGAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTN
                     FILRRSIIEEIEGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQ
                     RTRWAKGNIYVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTTVIISDILCVLSISK
                     LVEISIPINFFLIWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFV
                     AIKGMIEYLKDIIFKREVKWYKTERF"
     gene            44925..47006
                     /locus_tag="KPH29_000800"
     CDS             44925..47006
                     /locus_tag="KPH29_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBG1466430.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKVENSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASSFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSIKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSTKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGTNINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSN
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSMNEKNLYLGMDYLLNKSKVN
                     DLKGDTLIIDEYGEVEDLAYNLKPKKEVKDSSWNMSINKTTKVFLMISFITIIVVMIL
                     SMLYIKKYKRR"
     gene            47043..48128
                     /locus_tag="KPH29_000801"
     CDS             47043..48128
                     /locus_tag="KPH29_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBG1466431.1"
                     /translation="MRKYMNKFKLKRSILISLIILIFIFIVYFVSIELKDGSLVNNQY
                     KGKIKSANLSVDYEINQVMKDIDKLGLNTVNVPIIINVESINSDIMSIDNNSKEKAIK
                     LIKKLNKKGISTILEAYPWVENGKLYETDWNPINKKRFFYTWQNAILNELIIDVANPL
                     DVNVLNIGSNFVHLEEYQQNWGEIIDFVQSKFDGLVTYRTNWWYTKKDDSSSKLFYEQ
                     KLNNNFFDKLDFISIAAYFELSNKPVNTVDELISALHSSTVNNRGQNIKQEIYNLYKA
                     HRKPIFFGELGFSNRESASSQPWNHTPSKAVNGEEQARCFEAYKKVFENEDWINGFSV
                     FCVGKIDDEKNFYPSKESIKVIKSWYE"
     gene            48182..49306
                     /gene="wecB"
                     /locus_tag="KPH29_000802"
     CDS             48182..49306
                     /gene="wecB"
                     /locus_tag="KPH29_000802"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBG1466432.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENIKSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKALVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLARKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPEKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGIQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDSYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            49384..50358
                     /locus_tag="KPH29_000803"
     CDS             49384..50358
                     /locus_tag="KPH29_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBG1466433.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNDKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSVTIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQALENAGYSTAEDKKGNRIYAELLIDIIRSYNLQLIDNKVETK"
BASE COUNT        20109 a         5128 c         8743 g        16521 t
ORIGIN      
        1 attattataa atgaaaaaga aaaatttaaa gtactcactg ataatatatt aggaggggtt
       61 aaaacctgtt taaaagatga atatatgact atgacatatg taagtcaggg gtttttagag
      121 cttacaggtt actcttgtga agatttaaat aaaatattta aaaatagatt taaagacatg
      181 atttataaag atgatttaga caagtttaat tgtattattc taaatcaatt agaaaaacaa
      241 gataaaattg aagttgaata tagaattgta agaaaagatg gaagtataat ttggattttg
      301 gagagaagta agtctataaa tgataataaa ggaagtgagt gtattcaggg agttttaact
      361 gatataacag aacttaagaa aatacaagaa gagttagatt caaaaagatt tgaaatacag
      421 gctatcaatg atagtattgt tggtggaata gtgataactg agataaatga tgattttgat
      481 agtatatatt taaatgaagg ttatttaaat atgattggat atacacgcaa acaactggaa
      541 gatgagctaa ataataaatt aatcaaactt atttttaaag atgatagaac agaattaatt
      601 aatagtataa acaaacaact caaattaagt gatacatttg catctgaaca aagagttgaa
      661 aaaagggatg gtactattat atggatttta ttaaaaggaa gaaagataat ttatgataat
      721 ggaaaatctg tactttgttg tgttattgta gatatcactt ctgctaaatt attagagcaa
      781 gaacttaaaa agagtgaaga acgtcatgaa atcattctaa agcagacgca aaatatcata
      841 atagaatggg atatagaaaa agataaaatg aatatatcac ctaattggta taataagttt
      901 aaatatgacc ctataactga aaacgcaaca aaaaatataa ttaaatcagt agatatatat
      961 catgatgata tggataagtt tatttcatta attaaactta cttcacaagg tagtgagtat
     1021 gaagagtgtg aaattagact tagaactaga gatactattt acgcttggtt tagtataaag
     1081 gcaacaactt tatttgataa taatggtaaa ccttataaag tagtaggaag tttgactgat
     1141 attgatgaat caaaacgaga agctgaaaag ttgaaagaaa gggcagaaaa agaccctctt
     1201 acagatttgt ataataaaaa agcatcacaa tcacttatag aagagtacat aacattggat
     1261 aaaaaaagaa aaggtgcttt aatgattatt gatattgata actttaaagg aataaatgat
     1321 aatttaggac atttatatgg ggatgctgta ttaagtgaaa ttgcaaatga cctaataaat
     1381 ctatttagag caaccgatat agtaggcaga attggtggag atgagtttat tgtatttata
     1441 aaagatattt ctgaaacaag agatattata aataaagcag aagaattggt aaaaacattt
     1501 gaaaggtctt ttttaggtaa aggacgaaat tataaaatat cattaagtgt aggaattgct
     1561 aagtttccac aagatggtat ggactatatg acactattta gaaatgcgga tatggctctt
     1621 tactcagcca aagggaaggg taagaattgt tatacattgt atgataaaaa aattgataaa
     1681 gtccaatata taactaatac aagaaatgca ggagatagtg ataatgtaga gatgtctttt
     1741 aaagaacata tcacaaatta tatatttgat atactatatg aatcaagtga tatagaagtg
     1801 gcagtaaatt taattctaag tattgtaggt aaacacttta atgtgagtcg catgtatata
     1861 tttgaaaatt cagatgataa tcgagtatgt aaaaatacat ttgaatggtg taacgaaggt
     1921 gtaacctcgg aaattaataa tcttaaaaat atttgttaca aggatttagg caactatctt
     1981 ttaaatttta atgcagatgg gttattttat tgtaatgata ttgaaaattt aggtagtgat
     2041 gtgaaaaaag tacttaagcc acaaggcata cgttcaatat tacaatgtaa aattttagag
     2101 ggcaatgaat ttagagggtt cataggattt gatgaatgtt catataatcg tatgtggact
     2161 aatgatgaag tgaacacttt gacatttata tctaaaacgc tcagtatgtt tttattaaaa
     2221 atgcgtattc agagtaagct agaagagtct ttaacaattc aaaaatctgt gatggataat
     2281 atggatatat gggcatatgt tgttgacagg aatacctatg aattattatt tatcaataaa
     2341 aaaatgcttg agtcagcatc aaacactcat gtaggtgatt tatgttataa agcgatatgg
     2401 aataatcaag ataaaccttg taaaaaatgc cctatgatat atttaaaaga tggtgatgat
     2461 acttataaat catttatgcg caataaaaag tttgaggtac aggcaaatat aactacaact
     2521 actttgaaat ggtttgatgg gaaagatgca tgtttaatat ttggaagtga agtaactagt
     2581 attgaagctg aagaaaatac ttaaaatact gtgttatata tatacttaaa atcgcaattt
     2641 gaatttatta gacttaaaat gtctatataa atcatttaag ttgtggtttt aagtattttt
     2701 tatgatataa ttaagcgtta agaacaaaat taaaagatac ttttgtataa aaaaatattt
     2761 gttaaatata tattgagcag gtgataatat ggaaaataag tttttaccaa taagcaaaca
     2821 agatatgata gatagaggat gggaagaatt agattttgtt ctagttacag gagatgctta
     2881 tgtggaccat catagttttg gaacagcaat aatatctaga gttttagaaa atgctgggta
     2941 taaagttgga ataatagctc aaccagactg gagaactaca gatgatttta tgaagctagg
     3001 aaaaccgaga cttgcatttc tagttaatgc aggaaatatg gattcaatgg tcaatcatta
     3061 ttctgtaagt aaaaagcata gagaaaagga tatgtattct ccaggtggga aaatgggtta
     3121 tagaccagat agagctacta tagtatattg caataaaata agagaagcat acagtgatgt
     3181 tgcaatagta ataggtggaa ttgaagcgag tttaagaaga tttgcacact atgattattg
     3241 gagtgataag gttagaaagt ctatgttaat agatagtgga gcagatttat tagtttatgg
     3301 tatgagtgaa aagcagattg tagaagtagc caatgcactt aatgatggat atgacccaaa
     3361 atatattaga catattgatg ggacatgcta tatttcagat acattggaag aaatatatga
     3421 taaatatata ttaattccat catataaaga aatttgtgaa gataaaatga agtatgtaga
     3481 agcttttaaa attcaatatg atgagcaaga cccatttaga ggtaatataa ttgttcaacc
     3541 tcatggaagt aagtatctgg ttcaaaataa gccagaaaaa ccacttagta gagaagaact
     3601 agatgaagta tatggattac catatcagaa gacataccat cctgtgtatg aaaaatttgg
     3661 aggaatacca gctatagaag aagtaaaatt tagtatagta agttcaagag gctgttttgg
     3721 tagttgttca ttttgtgcaa taacatttca tcaaggtaga gcagtacaaa gtagaagtga
     3781 aaaatcaatt attgatgaag caataggaat aacaaattta gatgatttta aagggtatat
     3841 acatgatgtt ggaggtccaa cagctaattt tagaagacca gcatgtaaaa aacaaattac
     3901 taaaggtgct tgtaaaaata gacaatgctt atctccatca ccatgtaaaa atttagatgc
     3961 agaccacagt gaatacttac atttacttag agctgtaaga aaattaccta aaataaaaaa
     4021 ggtgtttgtg cgttctggaa ttagatacga ttatgtaatg gcagataaga acaataaatt
     4081 tcttagagag ttgatagagc atcatgtaag tggtcagtta aaggtagccc cagaacatat
     4141 atcagaagaa gttttaaagt atatgcaaaa accagcagga aaaacttatg ataaatttag
     4201 acagaagttt tttgctataa atgaagaatt aggtaaaaag caatatttga taccctattt
     4261 aatgtctagt caccctggtt ctaccttaaa tagcgctata gagttagcag agtatttaag
     4321 agatactcac taccaaccag agcaggttca ggatttttat ccaactccag ggactctatc
     4381 tacaaccatg ttttatacag gtattgaccc gcttacaatg aagccagttt atgttcctaa
     4441 atcaaaacgt gacaaggcaa tgcaaagggc tttattacaa tatagagcac ctagaaatta
     4501 tgatttggta tactctgcgt tagttgaagc aggaagagaa gacttaattg gttttggaca
     4561 tagatgtctt ataaagccaa aaaatgaaaa gccttatttt aatagaaata attctaaaaa
     4621 aaatgtatct aaaggaacaa ataaaaacaa aaaaactaat acaaataaca gaagtaataa
     4681 aaatcaacaa aaaagttcaa ctaaaaaaaa gaaaaaacat taaagtataa ataaaaatac
     4741 ttaaaaaaga aggattattc gataaattaa cagaaattag tataaataaa tataattatt
     4801 ttaatgacgt tacctttggg aggaagagat gaaaaagtat atttctataa tattattagt
     4861 ggtggtgact atggttgttt tggtaggatg ttcaccagga aaagataacc ctaaaaataa
     4921 ggagctatca gttgtcaaac aagtaaaagt agctgttcca gatggactac cagcagtggc
     4981 aatagctaaa cttgcaaatg aaaatccaga gattaaagaa ggatatgaaa cagtgtatag
     5041 cattgaaaag acacctgagg cgatttccac tagagtgatg aaaaaagatg cagatatagc
     5101 tatagttcct tcaaacatgg ctgcaatagc gtataataaa acttcatcat ataatattgt
     5161 tggaactgta ggaatgggtt cactttattt ggtgtcaaca gaaaatataa aagattattc
     5221 tgatttagtt ggtaaagaag ttggatgtac tggaaaagga cttactccag acataactat
     5281 aaaaagtctt ttgttacaaa aggatataaa ttattcaaat ataaaattta attatgtaaa
     5341 ttcagcaagt gaactggtac cattattggc tactggtaaa gttaagacag gtattgttcc
     5401 agaacctgca cttagtgctt tgatgtctaa aaatccagac ataaaaatta taaaaagttt
     5461 aaatgactca tggaaggaag taagtggttc aaaagacggt tatcctcaat ctactttaat
     5521 tgtaaagtct gaatttttaa gagataataa agattttgtt gattcttttg taggtcaatt
     5581 atcaaatagt atagattggg caaacaaaaa tcctgaagaa ttaggtgcat attctgaaaa
     5641 aataaaaata tctactgaat caaagataat aggaaaatct cttgaaagag ctaatttaaa
     5701 atatattcca gttaaggaca tgattaaaga ttataaaaat tactatgaga aattagctaa
     5761 ttttgatgat aagacattag gaggcaaagt accagatgaa gcaatatact ttgttgagaa
     5821 ataaaaaaaa taaagaacaa tttatagata aaatggaggt aacactttct tgtgttatcc
     5881 tcttattttt atggcaaatt attgctttaa aaattgataa tgatattttt ttaccaacag
     5941 taggtcaagt atttaatagt ataaaagaga ttatgctaaa ttctagtttc tatatagata
     6001 tactttactc tatggggaga tgttttttta gttttttact ggctatgatt tttgccatta
     6061 tactttcaat agtatcttat ttaaatagat tttttagaaa cttattaaag ccaatcaatg
     6121 ctttgacacg ttcaatacca actatgatat tggtagtact tgcacttatt tggtttgaaa
     6181 aggatagtac cccttttata gttggattta ctatagtttt tcctatattg tatgataacg
     6241 ttttgggcgc tatattgaat atagacaaga acttattaga aatggctaat gtgtataaaa
     6301 taaggtttat agataaggtg ttaaaaattt atttaccagc cataaaattt caaattatat
     6361 ctatactagt atcaacattc tctttaagtt tgaaagttgc tatagcagga gaggtatata
     6421 gtcaacctac atatggtatt ggaactatga tacagactga aaaaattaac tttaatacat
     6481 ctggaatatt tgcatggata attattgttc ttttaatatc tgctatttta caaatagccc
     6541 aaaagttttt agcaaggaga gcttttttat ggaaaagata aagttgagta ttgaaaatat
     6601 aaataaaaga tatgatagtc gtattatatt tagagatttt aatattgatt tttatgtaaa
     6661 tgaggtaaat tgtatccttg gaaaatctgg ttgtggaaaa actacactct tgaacataat
     6721 aagtgggatt attaaaaatg acaccaacaa tctaaatatc aaagaaaatt taaatagagt
     6781 aggaaataag cttgaagcta gttacatatt tcaagatgat agacttatag actggttaac
     6841 agtagaagaa aatataaaga tagttgtaaa taaatactat aataaaactc aattaaataa
     6901 aatttgtgat gaatacttgg aattggttgg aattagtgat tataaaaaat tctatcctca
     6961 gatgcttagt ggaggtatta gacaaagagt aaatattgct agagctttta tatatccatc
     7021 aaaaaacatt ataatggatg aaccttttaa atctattgat gcaaagaata cacagcttat
     7081 aatggataat ttcagaaata tattaagaaa agaaaagaga actgtattat ttgtaactca
     7141 taatattgag gaagcattgt ttttagcaga taggatattt attttaggag actctccaat
     7201 tagaataaaa aaaatattaa aaaattctaa ggaattggaa aaaaatgaag ttttgaagtt
     7261 gatatagagt tataataata ctgtcgtttt aaaatatatt tggttaaaat ctataagact
     7321 atattaaaat agtacagaat attcaaaact gtgtataatg ttcgtatttt ataaaaagga
     7381 agtgaatgta tggttaagac taatgctatg agaatattag attcaaataa gattgattat
     7441 aaagtaatgt cttatgaagt gaaaagtgaa catgtggatg gtgttgaggt ggcacatgat
     7501 ataggaagag atgtaaatga agtgtataag acattagtaa ctcaaggagt tagtaagaat
     7561 atatatgtat atgtaatacc agtacatgaa aacttagact taaaaaaggc tgcaaaagta
     7621 gctaaagaga aaagtgtaga gatgattcat gtaaaagata taaataagct tacaggttat
     7681 attagaggcg gatgttctcc tataggtatg aaaaagcttt ataaaacttt tgtaaatgaa
     7741 agtgcaaaga atttagatac aattattgtg agtgcaggaa aaataggata tcaaatagaa
     7801 ctatctccat ttgatttaca aagacttata aaagtagaat ttgtagatgt aataaaaaaa
     7861 taagaattac atataaaata tagagcttat attcttaata ataggtgttg taatattaat
     7921 aaaatgaata ataattttaa aataaatatt cagttactta tgtgtaattg gtataaaaat
     7981 attgagaaat tttaaaaaca atggtaaagt gtaagtataa tcacgaatat tttttataaa
     8041 gtaatggagg gatgttcgct tgagaaaaat aaaatctgaa caaatcgtcg agcaggtaaa
     8101 aaaactttgt atagaagcga gcctatattt aggagaagat gttttaagtt gtataaaaga
     8161 aaaagcgaaa agcgaaaaga gtgaagttgg aaaaaatata ttgaatatat tagttgaaaa
     8221 tgctgaaata gctaaagaaa aaaatatacc aatatgccaa gatactggta tggctgtatt
     8281 ttttgttgaa ataggacaag aagttttaat tgaaggagac acacttactg atgctataaa
     8341 tgagggagta agacaagggt atgaagaagg ctacttaaga aaatctgttg taagtccaat
     8401 taacagagta aatacaaaag ataatacacc tgctgttatt cattatgata tggtaaaagg
     8461 tgataaaatt aaaatagaat ttgcagctaa gggatttgga agcgaaaata tgagtaagat
     8521 gaaaatgtta aagccatcag atggattgga aggaattaaa aaattcataa tagatacagt
     8581 ttcagaagct ggacctaacc catgccctcc tatggtaatt ggagttggta taggtgggac
     8641 tgtggacaaa tgtgctcaaa tagctaaaaa ggcacttttt agagaactag gtgaatttaa
     8701 taaggacgaa aatatagcaa aacttgaaag tgaactatta acagctatta ataaacttgg
     8761 tataggacct caaggcttag gtggtactac aacagctttg ggattaaaca tagagacttt
     8821 tcctacacac atagcaggtc tacctgttgt tgtaaatata aactgccatg catcaagaca
     8881 taagaaggta gtaatttagg aggaaaataa aatgataaaa ataactacac ctgtaaatga
     8941 aattgatata gcaaaattaa attgtggaga tacaattagt ttgagtggta tactttatac
     9001 agcaagagat gctgctcata aaagattgat agattgtata aataaaggtg aagaattacc
     9061 atttgacgta tatggtcaag gaatatatta tgtaggacct actcctacaa agccaggaga
     9121 agttataggt gctgcaggtc caacgacgag ttatagaatg gatgatttga ctatacctct
     9181 tttagaaaga ggtcttagat tgatgatagg caagggaaaa agaagtgatg aagttataga
     9241 aggtatgcaa aaatacggag cagtttatct tgctgcaata ggaggagctg gagcgtatat
     9301 ttcaaattca ataaaatcat gtgaaattat tgcatatgaa gatttaggag ctgaagctat
     9361 aagaaagatt gtagtagaag accttaaact tacagtagca atagatagct atggaaataa
     9421 tatatatgag caaggtcgag caatttatga atgtaaataa tgtttagaaa taaatatgaa
     9481 tatcaaaatt aaaaatagtt gtgtatattt taaggaggaa aattgatgtc taaggattat
     9541 gcaaaattgg cattagaaat gcatgaaatc aacaaaggga aagtaagtgt agaaagcaag
     9601 gtagaagtta aaactaagga tgatttatca acagcatata caccaggggt tgctgaacct
     9661 tgtttaaaaa tacatgaaaa tcaagatgat gtttatatat acacttctaa agggaatcta
     9721 gtagcagtag ttagtgatgg aagtgctgta cttggattag gtaacattgg tgctgaagca
     9781 tctatacctg ttatggaagg taaagctata ttatttaaac aatttgcaga tgtagacgct
     9841 tttccaatat gtttaaagac taatgatgta gatgagatag ttaaatctgt agagttgatg
     9901 gagcctgtat ttggaggtat aaatttggaa gacatatctt ctccaagatg ttttgagata
     9961 gaagagagat taaaaaaatc tttaagtata cctgtttttc atgatgacca acatgggaca
    10021 gcaataatag ttgcagcagc cataataaac tcaataaagt taattgaaaa taaaaaaata
    10081 gaagatttag aaatagttat aaacggagca ggagctgctg gtatagccat agcaaagata
    10141 ctacttaata tgaatgttaa aaatataatc ttatgtgata gaagtggagc attggaagct
    10201 agtatagaaa atctaaacta tgttcaaaaa gaaatgttaa aagtaacaaa tataagaaat
    10261 gaaaaaggac ctttaaaaga tattataaaa ggaaaagatg tatttattgg tgtttcaggt
    10321 ccaggagctg ttacaaagga aatggttgaa agtatgtctg agaagccaat aatattggca
    10381 atggcaaatc ctactccaga gataatgcca gaagaagcaa aattaggtgg agcatttata
    10441 atgggaacag gtcgttcaga cttccctaat caagtcaata atgtactggc ttttcctgga
    10501 atatttagag gagcattaga tgttaaggct aaagagataa atgaagaaat gaagatagct
    10561 gctgcatatg caattgctaa tacaataagt gatgaagaaa tatgcccaga gtatatatta
    10621 ccagatgtat ttaataagaa tgtagtaaaa aatgttgcta aggcagttaa agaagcagcg
    10681 ataaaaacta aagtaaatag aatataaatt tttataactt attaaaaact agaaaagtat
    10741 aaaagactag tgaaattatg tacagtggga tgattttatt agtcttttta ttttaaatat
    10801 gacacaataa tgtcatatta agcttgatat aattttaata tggttaggag ccaaagaaaa
    10861 atataaggag tgatttaaaa tggaagaaac taaattttgc caatgctgtg caatgccaat
    10921 gggacaaact gatgaattat atgggacaaa taaagatgga agtaaaagta ctgattattg
    10981 tagttattgt tttaaagatg gtgagtttac agctgatata agtatggaag agatgataga
    11041 agtttgtatc cctcatatgc ttcaaagtaa taaggatatg actgaagatg aagcaagaac
    11101 aatgatgaac aatttctttc caacattaaa acgctggaaa taattttatg caaaattagt
    11161 tatatgcaaa acatgaaata tggtgttgag aataaggggt ggtgtgcttt atagcatacc
    11221 acctgttttg ttacttgcta gttcagttat tcttgtatgc ttaaatatgt tctcatctct
    11281 ctttctattt taaattgtaa aaatctaaaa tatgttattt atattctact tctttatcca
    11341 tgtctttttc tacctcaagt caaatacaac tacaaaatca tcattttcaa ttcactgttt
    11401 aatagaatta acagataaag agttataaaa ttttctattt tttatataaa ataatacagg
    11461 tcaagctgac ttctagctac ttgggatagt gtatatattt atgatagtta taggagtaat
    11521 accaatcatt agactgttga tttgtgtagt cttttgtaaa aacatttgtt ttaatatatt
    11581 gagtttttga aaaagtaaaa ttataattat aataaattaa gtgtaataat gtattagaag
    11641 taaaaataat taaaatagag gtgattaaat gagcagtata ttgttagttg aagatgattt
    11701 aagcttgata tatggattag agttctctat acaaaaaagt ggatttagta tagacatagc
    11761 aaggacagta aaagaagcta tgcaaatgta tgaggaaaaa aattatgact tattgctttt
    11821 agatgtatcc ctacctgatg gtaatggatt tcagatttgc gaaaaagtac gagaggtttc
    11881 taatataccc attatatttt tgactgcttc agatgaagag gtaaatgtag tgatgggtct
    11941 tgatatgggt ggcgacgatt atataacaaa accttttaaa ttaaatgaat taatttcaag
    12001 aataaaagca ttacttagac gttataatgt tgcaagtaat gtgaatgaat taagttcaaa
    12061 taatattact attaaattac ttgaaaatcg tgtttttaaa ggtgaatttg aggtggaact
    12121 tactacagca gaatataaat tattgtgctt atttatgaaa aataaaaata ttgtacttac
    12181 tagaaaaaac atattggaca agttatggga tggaaatggt agctttatag atgacaatac
    12241 attatcagta tatgtaagac gattgagaaa taaaattgaa gataatcctg aaaatcctaa
    12301 gtttctattg accgttagaa gaatgggtta taagtggaat gtaattaagt gagggtatta
    12361 atatgaagat attttctaat aaagatataa aaatattttt tttaattctt atatctattt
    12421 tagtaatgtt tattatagta gggcaaatag tggttataag tataactaac aattataaaa
    12481 aaacgttgat agaacatgat tataatatag ctggatactt atatagtata ggtgttgata
    12541 aatctaaaat accatcagca tttgttgcac atgaagataa atatatttta aaaggaaaag
    12601 aaatactaga tacagctgga tatggatttg atatagatgt tagctttaca ccaagtgtaa
    12661 aattgtttca aagtaaatat ataaaaacta tgtctatcta tttattagta ttttttacta
    12721 tgattatttt catactatat atgtattttt ttaggcaaca aagtaagata gaagaggcta
    12781 gttataaaat tgatttattt atgaatggaa atataaatga aagactaaat acatatgaag
    12841 aaggtagctt atcaaaatta tttagttcaa tagatgttat gtcgacatct ttaaatacac
    12901 atatattaaa agaaaaacag agcagagaat ttcttaaaaa tacaatttcg gatatatcgc
    12961 atcaattaaa aactccactt acagctttaa taatgtacaa tcagatacta caagaagaaa
    13021 gtcataatag tgatgtcgtt gaagcttttg tacaaaaaag tcaaagtgaa ctagaacgca
    13081 ttgaaacatt gattaagaat ttactaaaga ttacaaagat agattcaaaa acaatagttc
    13141 tcaataaaaa taatgttaat cttaaaaatt taattgtcaa aattttgtat agctttgaaa
    13201 ctagggcaga aaaagaagga aaggttattt ctataaatgg ttcagaaaat acattgttgt
    13261 tctgcgatta tgaatggatg tcagaggctt taagtaatct tataaagaat gcattagata
    13321 atacaaaaga gaatgataaa ataacgatag agtggtttga gacgactata accaccattg
    13381 tttcagttaa agacactgga aatggaatcc atgttgagga tattcatcat atatttaaaa
    13441 ggttttatag aagtaagttt tcaaaagata atcaaggttt agggctggga ttaccactag
    13501 ttaaatctat tgtggaacaa cataatggta ctatttctgt agaaagtaaa ctatcacaag
    13561 gaagtacatt caccataagt tttttaaagc ttactaatat gtaagataaa atacatcttg
    13621 cagtaaggta tatagattat tattatatta atcaattaga aaggtgagag agaaaatgga
    13681 tattcttgtg gttaataatt tatgtaaaac atatggaaat ggatatacaa aagtagaggc
    13741 attgaaagat gtatccttta cagtttcaaa aggagagttt gtgactgtag ttggagaatc
    13801 tggttcagga aagagtacac tattaaatct cttaggaggt cttgatactc caacatcagg
    13861 gagaatattt attgatggta aagaaatatg ttcaatgaaa gaggaaaaac agactatttt
    13921 tagacgtcgt aacatagggt ttatatttca ggcatataac cttataccag aactaactgt
    13981 ggagcaaaat ataatattcc cattgttatt agattataaa aaaccagata ctatttactt
    14041 agatgaagta ttgactattt taggtcttaa agataggaaa aatcatcttc caaatcaatt
    14101 atctggtggt caacaacaga gagttgcaat tggaagggct ttgattacaa agccaagtat
    14161 tatactggca gatgagccaa cgggaaatct tgacagtaaa aatagtaatg atgtaatttc
    14221 acttttaaaa gtgactgcag aaaagtataa tcaaaccatt attatgataa ctcataatac
    14281 gaatatagct tctacagcag atagaatact acaggtttct gatggtgtgc ttacagacct
    14341 gggggtgaat agagaatgag aagctactta gaattagtac cacaatatgc aaaagtacat
    14401 aagaaaaaaa atagaactac aattttatgt attattattg cagtatgctt agttactgct
    14461 atctttggac ttgcagatat ggctatacaa tgtcaaaaga tacaatttat taaagatgat
    14521 ggaaattggc acatagtaat tagaaatatt gatgaaaagt ttatatcaga aataaatagt
    14581 agagtagata tagctcacca tggtaggggg caagtcgttg acagtggaga gtttaaatca
    14641 aaggagattt tgatacaagg tttagatgaa gaagtagcaa aacaatttaa cttagaaata
    14701 ataaggggtc gttatcctat tggggagaat gagactttaa tagatgagga tgcagttaaa
    14761 agctttaatc taaaaatagg agattttatc aaaataaaat tagcagatgg tagaaataaa
    14821 aattataaga tagtaggtga atattcatca atatcaagct taagagctac agataaacat
    14881 ggattaatac tttcaatatt gggtacacaa gctattaaaa aatcagaaac gtacgattat
    14941 tattatatta catttaaaga aaaggttaat attagaaaag ccacttctga tattaaaaat
    15001 accttcaatt taagtgataa acaaatcggt aaaaatgaaa gattactttc tattataggt
    15061 caaggcgact ctgacttttc taaaccactg tatttaacag caggattact ttttgtactt
    15121 gttttagtag caagtattat catgatatat aatagtttta acatgagtgt aattgagaga
    15181 gttaaatttt ttggtctgtt acgttgtcta gggtcatcaa aacatcaagt taaaaaattt
    15241 gttgttttag aaagtttaat tttaagtata aaagctattc caataggctt agtattagga
    15301 agtcttgtaa ctattatatc ttctatattt cttaagtatg ttaatgagtt attcagctca
    15361 atgccaattt taaaagttag ttttattggt attgcatttg gaataattgt agggttttta
    15421 actgtaatat tatcagctat tattccagca aaaaaagctt cccgtgtatc tccacttagc
    15481 gctgtacgtg ggaatctaat gtccaaagaa attttaaata acgggaagac aagtaaagtt
    15541 gcaaatacag gaagttttaa aattgagact tctatgggat taaatcatgc tttttcaaga
    15601 aagaaatctt ttgtacttat gacttcttca tttgccatta gtattatact atttttaggt
    15661 tttagtgtgc ttatagacct tatgtatcaa ggacttaagc ctatgaagcc agaagctcct
    15721 catatatcta ttgttgaccc taattttaag tctgttttaa ctaaggaaga tattaatgaa
    15781 ataagcaata tcaaaggagt aaagaaagta tacagtagaa tgatgacgga tatagaagct
    15841 acatataatg ggaagaagac aaaatcacaa ttaatttctt atgaaaagta tcaatttaat
    15901 tgggcaaaaa aatatctatt aaaaggagaa gttaatgaaa ataaactaca gtcagaaaac
    15961 cttgcattag tagaagaggg gcatggattt gatataggag ataagatata tattaaaaat
    16021 agaaatgctg aaaaagagtt agaggtagga ggtatacttt cctcaattcc atttgaggct
    16081 aaagataaat atattggaaa tattataaca tctgaaaata cttttacgaa tataacaggt
    16141 ataaagggtt atataataat ggatattcag gttgataaag atactcctgg agatttaatt
    16201 gaaaatataa gacgtgtagt agatgataac ttagaggtca gagataaaag acaaagtaac
    16261 ttagaggtta aaaattcgtt ttatactatg gcaacatttg tatatggatt tgtgtttatt
    16321 attgcgatta taagcatgtt taatatcata aatagcatga atataagtgt tacaagtaga
    16381 aagaattact atggtgttat gcgtgcagta ggcatatcca acagacaatt aagaagaatg
    16441 gtaatagtag aggcaggttc atatgcaatg agtggttgtt tattaggtag tatactagga
    16501 ttaatgttac ataggtatat gtttatatcg ttagtaagtt caatgtttca tttagagtgg
    16561 caagtacctt ttaatttact acttatcact gtactaacta tgataataat aactttatta
    16621 tcagtaagaa aaccagtcaa aaagatttgt gaattagata taatagactc aataaatgca
    16681 caataaatat agctgttgat ttaaattaaa aaaacgaagt aaaaatgaaa tagaaaatat
    16741 ttttcatttt tacttcgttt tttatattat aaaatttttg ttatgttaca aaatttctat
    16801 aaagatgatg tgaatctaaa cattatatga caacgtgagc atttaagtta aaatatttta
    16861 aatatactat tttagataag ttccttcttc atcttgataa attaatcctt ttttcataag
    16921 cactccaaga gctcgtttaa aatttttctt acttgaatta aataatatag aaatatcttt
    16981 agggtcagat ttatcattaa atctcatata tccatcagaa ccctctaaat aacttaaaat
    17041 cttattttct aaagtatcaa gttcatcctt tctatttcct ctaggagaaa gaccaagttt
    17101 tccatcttca tatattttta tgacatgtaa attttcaaga acatcaccag cttttagttc
    17161 agtaaagtac tcattatgaa gtattacacc agcatattta ttgtctacac atatcattgc
    17221 agaattatta gtttgaaatc catatacaac tccagttaca atatctccta cattgtaagt
    17281 atggtctgtt aataaatatg aatctatatc tgtagtagca gcaattcttt tgcttttgtc
    17341 taaatatata taaaataaat atttttcgtc tctaaatagt ggataagtct tagctttaaa
    17401 aggaactagt atgtctttag gaagtcccat atctataaaa gtaccaatgt ccgtattatc
    17461 aactactttt aaatgtgcta catccccaac cttagctaaa ggcggtatta aagttgcagc
    17521 agttctgtca tcagaatctt taaatatgaa agcatttact tcatcaccta tgtttatttc
    17581 attttttccg attaatctat tatgaagaag tatatcgtct tttgtgttat tagtttgtcc
    17641 atccaaaaaa tatccaaatt cagtttttct ttttactgta agtttattaa aatcacctat
    17701 gtttatcaaa ttaaaatcat cctctcattt tatatttata atttagccat aatatactta
    17761 tgtagtagtt tgtcacagta tctatattag tattagtgaa atttacaata aataatatag
    17821 ttatttatgt atttttcaat tctctagctt cttatatagt ataaactcca atttcttttt
    17881 tgtctttagg ttttactaaa atttttacaa aataagttaa aaagtattga aataaaattt
    17941 cttttaaagt ataattggta tataccaaaa ttggtatata ccatatcgaa aagtggtgac
    18001 tatatgaaaa tagtaaataa atctagtaat ataccattgc atactcaatt aagttcaata
    18061 ataagagaga tgatagaaac tggtgagctt aaagagggtg atgcaataat gccagaaaga
    18121 gaactttgca acatacaaaa tgtaagtcgt atgacagtaa ataaaaccat tgttggatta
    18181 gtcacagaag gacttcttta tagggtacaa ggtaaaggga cattcgttgc aaagcaaaaa
    18241 aagaagtatc agttttcaaa tgtaaaaggt tttactgatg ttatgaaaga aaagggagta
    18301 aacataaaaa cggatatatt atcctttgag atggaactac cagatgattt agttaaaaga
    18361 aagcttggaa taagtgataa tactacaaat atatataaga ttgtcagact tagatatact
    18421 gatggagagc catttggatt agaaatagta tatctatcag aagaaatgtg taaaggtcta
    18481 acaaaaggga tattggataa ttcttcatta tatagggttt taaatgaaaa atatggctat
    18541 aagatacaaa aagctgaaca agtaatggag ccagtaatac tttcagatga agaaagtaag
    18601 cttcttgaaa cagatgaagg tgctttagct cttaaacttc atagaaattc ctataataga
    18661 gaaggtagcc ctatagaata taccatatca atatttagaa cagataaata tcaatatgaa
    18721 atagtattga gtgagtaggt gaataatatg aaatgtttaa ttaatggaaa gataatatta
    18781 aaaaatcaaa tcttggaaaa taaagtatta gtatttgatg aaaaaataat tgatatagct
    18841 gatagtgtac caaaagattg tgaagttata gatgctgatg gaaaatatat atctccaggg
    18901 cttattgata ttcatataca tggaaatatg ggaaaagata caatggattc gactgatgaa
    18961 tctattgaaa caatttcaaa atcaattatg agacatggag taacatcttt tcttccaact
    19021 acaatgacta tggataaaga acatgtttat gatgcacttg aagttattaa aaaagcccaa
    19081 aatagaaagc ttgaaggggc acaagtatta ggagcacatt tagaaggacc ttttataaat
    19141 gagaattata agggagcaca aaatgaaaaa tttataataa attcaaaata cgaatttata
    19201 aaagaatata aagatgttat aaaagttata acttatgctc cagaaaaaga tattgatttt
    19261 gattttacta gagaaataaa aagatgtaca gatatagttt tatctattgg tcatagtaat
    19321 gcaaattatg accaagctaa agaagctata aatttaggag taactaatat aactcacatg
    19381 ttcaatgcaa tgactggttt aaaccataga gacccaggcg ttgttggtgc agcacttaca
    19441 actaatgtgt acagtgagct aatagcagat acaatccata taaataaaga tttatttcaa
    19501 tttatactaa ataataaagg gaaagaaaga ctgattttaa taacagattc tatagaagct
    19561 ggtggtcttg aagatggcaa ttattctctt ggaggtcaga aagtcatagt taagggtaat
    19621 gaagctagac ttgaaaatgg agcattagct ggaagtgtgt tgtctttaaa taaaatggta
    19681 tttaacttct tagataatac aaaccttaaa gtgaatgaag caattaattt agcttcttta
    19741 aatccagcaa catctttggg aataaatgac aaaaaaggaa gcttagaaat aggtaaggat
    19801 gcagatatag ctgtatttga tgagaattta gattgtaaaa tgacactttg cttgggggac
    19861 gtagtatata aaaatatata gtatacaaaa caaactttaa agacagtaaa aacaatataa
    19921 aaaataataa attaaactta cattagttaa agtagtagtt aaaatttagg aggaataaga
    19981 tgagagtatt agtgtgtaaa gattatgatg gaatgagtaa aaaagccgct gaaatgatag
    20041 cagctcaaat agttttaaaa ccaaattcaa tactgggact agctacagga agtacaccag
    20101 taggtatgta tagagattta gtaaaaaaat acaatgataa tatagtagat ttttcagatg
    20161 tgatgagctt taatttagat gaatattata agttaccaat atctaatgac caaagctatg
    20221 attattttat gaaagagaat ctatttaatc atgtaaacat aaagctagaa aatactcatc
    20281 taccaaatgg aatggctgat gatattgaaa aagagtgtct gaactatgaa gcttctatag
    20341 atgcagctgg tggtatagat gtacaagtac ttggaatagg tcgtaatgct catataggtt
    20401 ttaatgaacc tgatactaaa tttgcaaaaa gaactcatgt agttgaactt acagagtcta
    20461 caatagaagc taatgcaaga ttctttaaat caagagaaga tgttcctaaa aaagctgtaa
    20521 gtatgggaat aggttcaata ttgaagtcta agaaaatatt gcttttagct tcaggagaag
    20581 aaaaagctga tgcagtatat aatactgttt acggagatat aactccagaa gttcctggtt
    20641 ctatacttca acttcataaa gacactatag ttatagtaga tgaagcagca gcaagtaaac
    20701 tgaatccaaa agattataaa ttagtataag aatagaatgt agtttaagtg aaaaatagct
    20761 atcattgaat aatcaaattt atttaatgat agctattttt atttaaattt tttacttctt
    20821 tttatttaaa ttagttatta ttgtttaaat tacttgctta ttacttttta cgaaaactcg
    20881 ttatcaagaa gtatccgaat aagatgttaa aaattattat gtttagtggg attctatcaa
    20941 gcataataaa attacctata attgggatat taccaattgg aaacataaag aagaatacag
    21001 gcatcagtga tagtaaaaaa gatggaaaac aaagaagaag taatcttgga atatcaatat
    21061 accaactacc atcctttttg tattctctaa tcaataattc agttcctagt aacaaaccaa
    21121 taccaccaga ggaaattaaa ttaataaaaa tgtatctttt aaagtcttgc atataaccag
    21181 tgtgaaattg agtatagaaa tcataattta atttaacggc taaaaaatat ataagcaata
    21241 gaatgattaa aaataaaaga tatctaagag cttttttcat tttaaattct cccttcagaa
    21301 atactttatt cagttttatt ttgttggaat ctgattttat catttagttt aagaaatcca
    21361 taatattagt gaagaatgaa ataagtatta tatatgaata aatattttta gaattaaggt
    21421 gaactgtagt gcttaatata actataatat taattgtttg aatattcaat aaaaagttta
    21481 aaaaatatca attttgatat aaactgaata aatacagttg aatattaaat tattatttta
    21541 tcaaaaatat gcaaagtact acaccttcaa atatcaccta aagtaattaa aaggcaatta
    21601 aagtgaagta gttcaagtat ttctaaaatg agtttaataa attctataat aatttatttt
    21661 ttgccttaaa attgccttaa aatccaaaca attttaaaac taatgtacta taaaaagctg
    21721 gtataattta aatatagtaa aaaataagat ttatcttatc tattgggatt taattattta
    21781 atcaatatat ttataccatt taatttaaat aaaaagtcta ttaaggagtg agcttatgag
    21841 taagttaatt tatatagcag atgatgaaga taatataaga aatttggtaa agacattctt
    21901 aaaaaatgaa ggtcatgatg ttatggattt taaaacgggt gatgaacttt tagaacaatt
    21961 taatataaag gagtgtgact tagtaatact agatataatg atgcctggct caagtggttt
    22021 tgaagtatgt acaaaacttc gtgagaaaag tacagtccca ataatcatgc ttactgcaag
    22081 agatacagat atagattata ttacagggat aaccttaggt agtgatgatt attttacaaa
    22141 accatttagc ccaatgtctt tggttatgag agtaaaatct atatttagaa ggatagagtt
    22201 tgaaaagaaa caaaattatg ataaatatag taactcgata gatatggaat taaaatttgg
    22261 tgatgtaata ataaataaaa agaataaaat cgttacaagt aaaaatgtaa atatagacct
    22321 tacaccaaat gaatataatc tattgactta tttgtttgaa aatatagata gagcagtttc
    22381 cagagatgaa ttattaaaca aaatatgggg atacgatata gaagttgaga caagggctgc
    22441 tgatgataca gtgaagagat tacgtaaaaa aattttagat acaaatattt taatagaaac
    22501 tgtatggggg tttggattta ggttgaagga gaaaagttaa gatgaataat ggaaatgaca
    22561 agaatagaat tggaaagatt aaatccaaat ttaaattaaa gttctcaaag aaaaatataa
    22621 gcataaaaac taggattttg ttgagcatac tttgtttgat aattatagta tttatgataa
    22681 tattagtatc ttttaatgta tttcttgata catatataaa aacaactgcg aatgaagaat
    22741 tgacgaagtc tacagaaata gtagaacata tggatagtaa ttttagacct attaagccac
    22801 cacaagatga tggaaagctt cctcccaaag tattatctaa ttttatgaga aatgtacaag
    22861 ataaagttag gatggcagaa acacaatcag atgctgatgc tatggtagtt gattctaagt
    22921 ataacctgat atttccaaca agagaggatg atttcttaaa aaatgttgat gagatggagc
    22981 tgattaggga gtgtattcaa aatgaaaaat tagatttaaa ttcagatgaa aatacacgga
    23041 tatctaccag aaataaggat tattatattt caactgtaaa aattactagt atatatggtg
    23101 aacaagacaa atatcttata ctttttatag acatatcaaa aactctaaat ctagcacaaa
    23161 agataaatat tgtattaata tctgtaatgt gttttgctgg aatattagca atatttacag
    23221 ctgtgatttt atctgagaaa attgctaaac cgataaaaga gttgtgtgag tttgcaaaaa
    23281 aaatgggtca aggagatttc aaaagaacct actttgactt ttcagataaa gaattagtag
    23341 agctctcaac tgttatgaat aagagtgctg agtatctgga taagtatgat aatgaacaaa
    23401 aaatattttt tcaaaatgca tctcatgaat taaggacacc actcatgtct attaaaggtt
    23461 atgcagaagc tataaaatat aatgttatag atagtaaaca tgcaagtgat ataattcttg
    23521 aagaaagtga taggttaagt gatatggttg aagatttatt atacatttct aaaatagata
    23581 atataactaa agattttgaa cttgtagaat gtgatttaag agaagtattt tcaaattgtg
    23641 gagctaggca aaatgtaaga gctataaata aggggataaa atttatttat gaatttgatg
    23701 aagaaatggt tttatttgaa tgtgatgaaa aaaacatttc caaagcattt atgaatctta
    23761 tagaaaatgc tttgagatac gctaagagtg aaataaaaat agtgtgtaag tataatcaaa
    23821 aaaatatagt tgtgataata gaagatgatg gaattggaat caagaaagaa gatttacctc
    23881 atgtttttga aagattctat aagggagtag ggggaaatca tggaattgga ttatcaatag
    23941 ttaaatctat tgtaaataag catggtgggc gtatatacgt agaaaatggc aaaaaaggag
    24001 ctaagtttac tatattattt aaattgtaaa aaaattagag aaaatataaa gatgtttgaa
    24061 gttgtttttt atatatatct gttgtatact gttgaaaaaa gaacaatatt aagctgacat
    24121 atattacaat aattgacatt taaagtaaat gcttaaatat agatacgaaa ggaaaagtaa
    24181 aatctgaagt gtatttaggt ttaagtaagc ttcttatttt ataaaaaata tgtatgaaag
    24241 aacagctgat ttttaatatt acaaatttag taacggttgt atttgaagct tttgtcatcc
    24301 atatgttttt atcagacttt ttaggtcaga aagaagggta tataaaagtt gtaagatatg
    24361 ctaaactggg ttttataata tgtttaggat tctgtaattt aataacttta aaccctaaaa
    24421 tcacaatgcc tttaattttt atccttattt tttcaacatc atttttatat aaaggaaatt
    24481 taaagacacg attgtttact actgttttat taagtatatt ctttatactg tcagaaattg
    24541 tagttacaag tatttttgtt ttatttgtaa aagaaggctt tgaaataatg cttgaaaata
    24601 atagtataag agtattggcg acaattttat ctaaaattgt ctttttgttg acttgtaaaa
    24661 ttatttgtct atttaaaaaa gatgtacatt tagatatgcc cataaagtat tggctccctt
    24721 tatttttaat acctatattt agtttatttc tgtctgtttc tatctttgat gtaagtaaat
    24781 tttttagtct tgaaagttta aaatttctaa gtttaatatc tagtgttggc attttatata
    24841 ttaattttat tgtattttat ttatttaaat ttattataga taaaactaaa ctatctatga
    24901 aatatgaaat acttgaaaaa gaaataattc ataaggaaga attaagactt agtaatgagt
    24961 gttataaaat tattgttgaa cagactgatt ctgtcgtttt cgaatggaat atcaaagaaa
    25021 ataaatcatt tgtatcgcag gcttggactg aaaaatttgg atataacaat gcatgtaaga
    25081 atatttttaa agaaattaaa gataaagact tagttcattc tgaagataaa gctatatttg
    25141 aaggttttct tgaaagtatt aaaaaaaaga atatacacag tcaagcagta tatcgtttaa
    25201 aaaaatctaa tggtgaatat atttggtgta gaacatcaat tacaagcatt tataatgatg
    25261 aaaatgaact tttaagagtt gtaggagtaa ttgtagacgt agatagtgat ataaaaaaat
    25321 atgaagagtt aagaactaga gcagaaagtg actcattgac caatatatac aataaaggta
    25381 catttgaaaa attagtggaa gaaactatag ttatgaatac aggtgataaa aaagatgcat
    25441 tattcattat tgatttagat gattttaaag aaataaatga taattttggt catccatttg
    25501 gtgattttgt actaaaaaca tttgctgata agatacaagc tagttttggt aataaagact
    25561 tagtcggaag aattggtgga gatgaatttg ttgtatatat gcaagattat gttacagaag
    25621 taaatttgca taaaaaagct aaagaattaa atagagtact ttcggataat tacactgatt
    25681 tgagttttag ttttgattct tctgtcagta taggtattgc aaggtatcct caagatggaa
    25741 cttcattttt tgaattgttt aaaaatgcag atagagcact ttatagcata aaagcatcag
    25801 gtaaaaattc atattgttta tttgaagaag aactatatgt acagtgaatt tgaatttaga
    25861 ttttatgtga gagtaagatt tatatatact tgtggctgtc tgatttataa aatatgttac
    25921 tttgtatatt tttatatgtt tcagtaaggt taattaacat aagcaaaggt gtaagtaaat
    25981 ttatattagt ttaagtttac ttacaccttt ataaaaataa attttgtaaa tttaaacagt
    26041 tggttttata aggattttta tttattttaa tatcttagat attccttttt gtattcctta
    26101 gagaaaataa ataatgaaag gagtacagaa aaaattaaac atcctaatat accacttgaa
    26161 ttaagtctta aagcattaaa gtttaaaaaa gcattattta aatcggtatc caaaagaaaa
    26221 ggatgctttg atttgctata tatggaaaaa ttaaaaatat attcataatc aaatttataa
    26281 aattaatatc tttccatcct aacattacat ttacttagat taattattat tataaaaata
    26341 aaaatacaaa taatagattc atttacattc ttaaatgatt aaagctcttt accctttaaa
    26401 catgcataac atatcgttat aattccagta aataagacta aaatgcccat tcctaaaatt
    26461 gcagtttcat catctattac tccaaatgct tttaaagtaa cttatttcgc tttgtataat
    26521 aataatttgt ctctttttat attttaactt ctgctgagat ttgagttaga tattcgttag
    26581 aatctcgcac acttatatca tatagaagat attcttcata aacaaaatcc ccaatacgta
    26641 aattattctg attaaaaaaa tttaataact tttcataagc cttgtaaata gtttcataac
    26701 tacctttatg ataagcagtt acataaagtc cctttggttt gattgataca ggatatttct
    26761 tgtactcttt attaactttt gtaaataaat atgaatatga ttcaaagtct ttattaatta
    26821 tatcttcacc tttaatgatt gagccaatcg taccatagtc actcaattca tagttgtcca
    26881 taaagttaat acattcatat aaaaaatttt cttcctcatc tcctaaaaac tgttctttat
    26941 atgtaatagg tgttttgacc aagtactctt cctcatgttc ttttaaagta atagtattcg
    27001 catctatatt acaggcattt ttagtgaatg aaatagtaga ttccattaga gcttgaatat
    27061 tatttagttt ttcaatttca tttttaatat caatggtttt cttttctaaa agctcaatta
    27121 gtaaattagg tgttcttttg tctatataag ctttaatttc ttttaaaggc atttttactt
    27181 ccctaagtga agaaattata ccaaaaatgc taagttgatg gtatgagtag tatctatatc
    27241 cattttcttt ctttaattct ggtgagaaaa gacctatatc atcataatgg aaaagtgttt
    27301 ttttattgat tccacaaatc tttgcaaatt ctccagttgt aaaataattt tttgaaactt
    27361 gactcataat tataattcct ccttgactat cctgttacag tatagtttat catagtattt
    27421 tgtaaaggag tgtgattata tgaaaaaatt aattcatttt ttaaaaccat atagagtttt
    27481 aattgttgta gttttgatat ttacgttttt acaaacactt ggaactttgt atataccaac
    27541 acttacagca aatatagtaa acaatggtgt agtaaaggga gatatagatt atattgtaaa
    27601 aacaggttta ataatgatga tagtagctgg tataacagct ttatcagctg tgttagtttg
    27661 taaagtatca gcaaatcttt catctggatt ttgtagagat attagagaag cagtatttgt
    27721 aaaatcacaa gatttatcaa taaatgattt taataatata ggtacagcat caatgattac
    27781 taggagtact agtgatatta ctttaatagg tcagtctgtg tttatgttta ttcaattggt
    27841 attaccagca ccaattataa ctgtttctgg tctattctta gcatattcta tagataaagc
    27901 aatgacaatt attattgttg tagtaatgtt tttattcatg ctttcagcat ttttagttgg
    27961 taaaaagctt ataaagttgt ttaaaatgat gcaaataaag atggacaata tgaatagggt
    28021 attacgtgag gtagtaactg gagtaagagt tataagagct tttaataggg agcattttga
    28081 aaaaaagaga tttgatagaa ctgcaataga ttattctgaa acagcaattt caataaataa
    28141 agtatttgca gtacttatgc ctattgtaat gttaataatg aatttaggta tagtaagtat
    28201 tatttggttt ggtgggatga gagtcagtaa tggaaatatg gagataggtc atataatggc
    28261 acttattgaa tattgtatct taattttatt ctatttaatc atgggagtaa tggtatttat
    28321 gtacatacct agagcaggtg catgtgcaga tagagtaaat cagatattag atatagaacc
    28381 tgaaattgta gatggaaatg gtcgtaaaga tactgtctca gagaggggac atttagtatt
    28441 taaaaatgta acattttcat atgcacaatc agaagaacct gtgttaaata atataacatt
    28501 tgaagcaaaa tctggagaag taactgcaat tataggaagc actggctcag gcaaatctac
    28561 tattgcaaat ataataccac gtttttttga aattcaatct ggagaaatat ctataaatgg
    28621 tcaggatgtt aagaagattc ctcaaaaaga acttagagat aagattggat ttgtgccaca
    28681 aaaagctttt ttatttagtg gaacaataga agaaaatata aggtatggaa aagaagatgc
    28741 aagtattgaa gaaataaagc atgctgcaag tatagctcaa gctgatgaat ttatttctga
    28801 tatggaagac aagtatgact catttgtagc acaaggtgga aataatttgt caggtggtca
    28861 aaaacagaga atatccattg caagagcgtt agttagaaag cctgaagtat atgtctttga
    28921 tgatagtttt tctgcattag attttaaaac agataaaaga cttagaaaag cgttgaaaaa
    28981 tgaaatcaaa gattcatcag ctataattat agcacaacgt ataagtacaa tcatggatgc
    29041 taatcagatt atagttttaa atgatggaaa aattgtaggt attggaaaac ataaagattt
    29101 acttgaaaat tgtgaagttt ataagcaaat tgcggattca caacttagta aagaagaact
    29161 tgcataaaat gaagaattaa tctgatattt ggaggtgtat gatatgagtg aaaaaagaat
    29221 agatgaacaa gatatagaag tacttagttt tgaagatggc atgtcagcag aaaaagttaa
    29281 ggatgcaaaa aaaactacta agagattatt aaaatatatg gcaaaacaaa agtttaaaat
    29341 attaattatt tttatttcag tattgataag ttcagcgctt acagttttag cacctatggt
    29401 tatgggaaaa gctattgacc aactatttaa tggaataaaa actgcagtcc aaacaggtac
    29461 aaaattcagt gtgaatttta gtactatggg ggggatagta tcaatacttc tagggttgta
    29521 cttaattagt gcagtattta tatatattca acagtatatt atgtcaggag ttgcacaaaa
    29581 tctagttctt agcatgagaa aagatttaag tgataaactc aataaacttc ctcttaagta
    29641 ctatgattct cataaaaagg gggaaacact tagtatagta acaaatgatt tagaaaaagt
    29701 agcagatagt ttacaagaag gattaatgca acttattaca gcagtagtta cagtagttgg
    29761 ttctattgta atgatgataa gtataagtgt accacttaca atagtatcag ctataacatt
    29821 acttgtttca ttgggtatta ctgttgtaat tgcaagaaaa tcacaagaaa gattctctga
    29881 aaatcaaaag gcactaggag agttaaatag caatatagaa gagatattta ctggtcaaat
    29941 agtggttaaa gcatttagta aagaaaagga tactataaga aactttaaaa tattaaatca
    30001 aaatctttat aatgcaagta gaagagcaca gttttcgtca tatgcaatat ctccaattat
    30061 tagatttata aacctaattg gctatgttat tatagcagtt gtaggtggga tatttgcatc
    30121 aactggagca atgactcttg gtagtataca agcgtttatt caatatgtaa atcaagcatc
    30181 agagcctact acagaaatat cttatattgt aaatatgctt caagcagcaa tagcatctgc
    30241 agagagagtt tttacagtta tggatgaggt ggaagagata aaggatgaag aatcttcaaa
    30301 ggttattagc atgcctaaag gtaaagtaca atttgagcat gttaaatttg gatatagtga
    30361 tgattttatt cttatgaaag atattaatat caatctaaat gcaggagaca agattgcaat
    30421 tgttggacct acaggagctg gaaagacaac tcttgtaaat ttacttatga gattttatga
    30481 gatacaaggt ggaagaatta ctattgatgg tgttaatata aaagacttaa agcgtggaga
    30541 acttagaact atgtttggta tggttcttca agatacatgg ctgtttaatg gaagtataaa
    30601 agaaaatatt gcatatagta agtcagatgc cacaatggat gaaattgtaa gagcagcaaa
    30661 atcagcaaga gtagaccatt ttataaggac tttaccacaa gggtatgaca ctatattgac
    30721 tgaggatgct tctaatctat ctcaaggtca aaagcaactt ctaacaattg ccagggcaat
    30781 actttcagac ccatcagtat tgattcttga tgaagcaaca tctagtgtag atactagaac
    30841 agaggtagaa attcaaaagg caatgaataa cttgatgaag ggaagaacta gttttgttat
    30901 tgcacataga ttatcaacta ttcgagatgc tgatttaata ttagtaatga aagaaggcac
    30961 tattattgaa caaggaagcc ataaggaact tattgaaaag aaaggattct atgaagaact
    31021 ttataatagt caattttcaa gtgaatatga tgaagaagtt gtatgattat caagttttag
    31081 gcaactaaat gattgataaa tattaagtta aatatagtaa catgtaaggt tatatggaat
    31141 aagagtattt ctctatttca tataaccttt tttatttatc aaaataagat aaaattttct
    31201 ttctaaatta gattgaagaa agtatttatc aatataaaag taaaataatc attattatta
    31261 gtgaagtaaa tattgtttgc cacatatatg ccataaagat gcacttattt tacggagatt
    31321 taacaaagta gtgtaaagta tactctaaat ataaaataaa tcaaatagag ttaaataaat
    31381 tgagaggtga tataaatagt gaataaaaaa ttgaactata gatttgaaat gaagcacaaa
    31441 ataactgaag cagatgtgtt agctttaaaa tcaagactct atccaataat gaagaaagat
    31501 gaaaatgcat ctaaagatgg aaaatatctt attaggagtt tatattttga tactcctgaa
    31561 gataaagcac ttttagataa attaaatggc gttgcaataa gagaaaagtt taggataaga
    31621 ttttataata ataactgctc ttatataagg ttagagaaaa aaatcaaaca ttacaacatg
    31681 acatcaaagc tatcaactaa tttaaccaaa aaagaagtaa taaatatact aaacaatgat
    31741 attgaatttc taaaagagtc gacaaattgt ttattaagag aattttattt gaaattaaaa
    31801 tgtgaaagat tagaggcaaa aagcatagtt gattacaata gagaagcttt tatttatcca
    31861 gttggaaatg taagagttac aatcgatagt gatattaaaa cttctgtaaa ttcgatagat
    31921 ttatttaata aagatttgcc gactgtttcg gtaattgatg agaatatgac tgtattggag
    31981 gttaaatatg atgaatttat accagatttt ataaaggatt taattcaaat taataagact
    32041 acttctacag cagtttcaaa gtatgcttca agtagattat atatttaaga ataaaggcta
    32101 tgatttatat aaagatatac ataaataagg ttaaaaaaca aataggttaa aaataaataa
    32161 aataaaaaat aaataagtta aaaataggaa ggatgatttt aattatgaca tttaatgata
    32221 tttttaaatc aagttttata gaaaacgtat caggtttttc atttgtggac agtgctttag
    32281 cactaggttc agctttttta gtaggtttgt ttatatatat ggtctataaa aagacttata
    32341 tgggaattat gtattctcga ccattcaatg tgtcgcttgt agcacttaca atgcttacaa
    32401 cttttgtaat attggcggtt acttcaaatg tagttttatc cttaggtatg gttggtgctt
    32461 tatctatagt aagatttaga acagcaatta aagacccaat ggatttagta tttctatttt
    32521 ggtcattggg ttcaggtata gttttaggag caggtttgat tcccttagct gtcatgggtt
    32581 caataattat gggattgatt ctaatattct tttcaaataa aacaatatct gaaactccat
    32641 atatattgat ggtaaattgt aataatgaag attcagaaga tatagcaact gataaaatta
    32701 aaaaagtatt tactaaatat caaataaaat caaaaagtgt tacaccagaa aaaggaatag
    32761 aattagtgtt tgaagtaaga atgaaatatg gagagacaag tcttataaat gacttgagtc
    32821 aagtagatgg agttactaat gcagttcttg taagctataa tggtgactat gtagcataga
    32881 gaggagtaat cttttatgaa agataaaaaa tttacccttc ttatctctat tatgattata
    32941 tttttatgtg ctgtagttgg agtttatagt acatctagca acaaaagtgt tgatttatat
    33001 agtgatgtat atattgaaaa atattttaac agagacaagg ttatggaagt taatatagag
    33061 atagatgaaa gtgacttgaa ggatatgaat gaaaatgcta taaaagaaga atttaaggtt
    33121 gcaaaagtaa ctgtagatgg agatacatat ggaaacgtag gtataagaac taaaggaaat
    33181 tcaagtctta catctgtagc aaatagtgat agtgatagat acagctataa gattaatttt
    33241 gataagtata atactagtca aagtatggaa gggcttactc aattaaatct taataactgt
    33301 tactctgacc catcttatat gagagagttt ttaacatata gtatttgcga ggaaatggga
    33361 ttagcgactc cagaatttgc atatgctaaa gtctctataa atggcgaata tcatggtttg
    33421 tatttggcag tagaaggatt aaaagagtct tatcttgaaa ataattttgg taatgtaact
    33481 ggagacttat ataagtcaga tgaaggaagc tcgttgcaat ataaaggaga tgacccagaa
    33541 agttactcaa acttaatcgt tgaaagtgat aaaaagacag ctgattggtc taaaattaca
    33601 aaactattaa aatctttgga tacaggtgaa gatattgaaa aatatcttga tgtagattct
    33661 gtccttaaaa atatagcaat aaatacagct ttattaaacc ttgatagcta tcaaggcagt
    33721 tttgcccata actattattt atatgagcaa gatggagtat tttctatgtt accatgggat
    33781 tttaatatgt catttggtgg atttagtggt tttggtggag gtagtcaatc tatagcaatt
    33841 gatgaaccta cgacaggtaa tttagaagac agacctctca tatcctcgtt attaaaaaat
    33901 gagacacaca aaacaaaata ccataaatat ctggaagaga tagtaacaaa atacctagat
    33961 tcagactatt tagagaatat gacaacaaaa ttgcatgaca tgatagcatc atatgtaaaa
    34021 gaagacccaa cagcatttta tacttatgaa gaatttgaaa aaaatataac atcttcaatt
    34081 gaagattcta gtgataataa gggatttggt aataaagggt ttgacaacaa taactctaat
    34141 aacagtgatt ctaataataa ttctaatagt gaaaataagc gctctggaaa tcaaagtgat
    34201 aaaaaagaag ttaatgctga attaacatca agcgtagtca aaactaatac agataatgaa
    34261 actgaaaata aaactacaaa tgatagcgaa agtaagaata atacagataa agataaaagt
    34321 ggaaatgata ataatcaaaa gctagaaggt cctaggggta aaggaggtaa gtcaatacca
    34381 ggggttttgg aagttgcaga agatatgagt aaaactataa aatctcaatt aagtggagaa
    34441 acttcttcga caaagcaaaa ctctggtgat gaaagttcaa gtggaattaa aggtagtgaa
    34501 aagtttgatg aggatatgag tggtatgcca gaaccacctg agggaatgga tggtaaaatg
    34561 ccaccaggaa tgggtaatat ggataaggga gatatgaatg gtaaaaatgg caatatgaat
    34621 atggatagaa atcaagataa tccaagagaa gctggaggtt ttggcaatag aggaggaggc
    34681 tctgtgagta aaacaacaac atacttcaaa ttaattttag gtggagcttc aatgataata
    34741 atgtcgatta tgttagtagg tgtatcaagg gtaaagagaa gaagatttat aaagtcaaaa
    34801 taagattaaa attgaatata aataatttta atactgttaa gtctaaatat ttacttagca
    34861 gtattttttt agaaaataat gtgtttgatt aataatataa ggtaataaat tgataaaaat
    34921 taaatacagc atattaataa tacataataa agtaataaaa aaactgttta agtataataa
    34981 cttatattta gacagttttt ttcatttata aataatataa atgaaattga aaacatttga
    35041 aggatagaaa ttggttggat aaatgtataa attacttcac caaaaaataa tgacaagagt
    35101 tgtaactaag ttgtaataaa atggtagtta tttagcataa aataatgtat catagaagta
    35161 tattataaaa ttaataaaat aagatttttg taagaaaact gctggctttt gatatttaga
    35221 gtgagtagtt aaattatgtt taggaggggt agaatttgtt gcaaaaagta ttatggaaat
    35281 ttttaaagct agtagtcggt ttatttatat gttcagttgg tatagtattg acgataaatt
    35341 gtaaccttgg attatctccc tgggatgtat ttcatcaagg actttcaaat catataggaa
    35401 taactatagg aactgcttct ataattgtag gctctatagt agttattgct gatgtcgttc
    35461 ttggagaaaa tgtaggatgg ggaactgttt ttaacatgct actaattgga ttctttatgg
    35521 atttactttt atatagtaat ttaattccag aggctgatag tttatttgta ggaataatta
    35581 tgttgatatt aggattggtt cttttgtctg ttggaatggt attttatatg ggaagtggtt
    35641 taggaagtgg acctagagat ggtttgatgg ttgcaattca aaagaaaaca ggaaaatcac
    35701 ttaaattaat aagaggtact atagaagttg gagcattaat agtaggattt ttattaggtg
    35761 gaaaagttgg tataggaact ataataagtg catttggatt ggggtatttc acacaaatgg
    35821 tatttggctt atttaaatta gattgtagta agattaaaca tagatttata gtagatgata
    35881 taaaatttat caaagtgtat atgagtggtg agaaaaaagc tgctgttcaa aatatagtta
    35941 agtaatttaa gaataaattc aacgaaatgt taagtatttt aaattaacat tttatttaga
    36001 tggataaatt gaatattaaa agaatattaa aatatgagga ttgctagaac actataaaat
    36061 atagatttct agcaattttt tatttgttca atgttttttt agtactagaa gtcttattct
    36121 ctatttaatg aatgaattaa tttataatta gagatactaa actttataga tattaatata
    36181 aacaccttta aactgtgtat tttccaaagc ctttaaaatg cttgtattta ttctaaataa
    36241 aaaaataaaa aaatgtcaaa aaaactcttg tattatatcc aaattttata tataattacc
    36301 tatgttgttt agaaatttta aactcaatgt ttagtaaaca aaatgtttag taaaataaaa
    36361 cagaaactag gaggtgcaac atggatatag gtgaaaagat aaaaagatta agaacagaaa
    36421 aacaactgac ccaggaagag ctagctaata gatgtgaatt gtcaaaggga tttatttctc
    36481 aattagaaaa taacttaaca tcaccatcca tagctaccct tatagatata cttgaaatac
    36541 taggtacaaa tttaagggaa ttttttaatg aaatagatga tgaaagaatc agttttacaa
    36601 aagaagatat gtttgaaact gaagatgaag atttaaaata taaattaaag tggttaattc
    36661 caaattcaca aaaaaatgaa atggagccaa taataataac attgtatcca ggaggacagt
    36721 ataaagaaga aaagccacat gaaggtgaag aatttggata tgtattagca ggttcaatat
    36781 atgtccatat aggcgaaaag aaaaataaag taaaaaaagg agagagtttt tactttagac
    36841 caaaagcaaa tcactatata tcaaacgaag gcaaaactac agcaaaagtg atttgggtaa
    36901 gtacgccacc atcattttag aatagaggtg tttaataatt ggtagaaaat ataatagaat
    36961 taagggatat atctaaacat tatgaggaac ttactgtttt agataatttc aatttagata
    37021 taaagaaaaa tgagttttta acattacttg gaccaagtgg atgtggaaaa actacaacat
    37081 taaaaataat agcaggattt gagtatgctg atgatggtaa agttttattt gaaggtaagg
    37141 agataaataa cttgccacca tatgaaagac aagtaaacac agtatttcaa aaatatgctt
    37201 tatttccaca catggatgtt tatgaaaatg tagcttttgg attaaaaatt aaaaagactc
    37261 caaaagatgt aatagatgct aaagtaaaag aggttttgaa acttgttgca ctagaaggtt
    37321 ttgaaagacg acaagttgaa tcattgagtg gaggacaaca acagagaatt gcaatagcaa
    37381 gagctctagt caatgaacca aaggtattat tactagatga accacttgga gcacttgatt
    37441 taaagttaag acaagagatg caaatagaat taaagagaat ccaaaaaaaa ttaggaataa
    37501 catttgtttt tgtaactcat gaccaagaag aggctcttac tatgtcagat actattgttg
    37561 taatgtataa gggcaaaatt caacaaatgg gaacaccaca agatatatac aatgaaccta
    37621 aaaattcatt tgtagctaaa tttataggtg aaagtaacat atttgatgga attatgatag
    37681 aagattataa agttaatttc tgtaatagag attttgagtg tgttgataaa ggattcgaaa
    37741 aaaatgaaaa tatagaagta gtaataagac cagaagatat aaaaatggta aagccagaag
    37801 aaggaatgtt aaaggggaaa gttacatcta cagtgtttaa aggagtgcat tacgagatag
    37861 aattgaatga aaatggaaga ttgtggatac ttcataatac caaaaatgct gaggttggga
    37921 ctgaactagg catggatata tatccagaag atatccatat aatgagaaaa gagaataact
    37981 aagatgaaaa gaaaatcttt tttagcatat ccatatgtag tttggagtgc gatatttgta
    38041 ataattcctt taatattagt ggtatttttt agttttacaa aagaatcagg tggaggttat
    38101 gcctttacac ttgagaatta taaagaagta attgacccta tatatatgaa agtttttggg
    38161 cgttctattc ttttagcagg aggagctaca ttaatatgcc ttatagttgg ttatccagtg
    38221 gcatatataa tttcaaaggc tcgtgtaagt agaagaggtt cattaatatt attgtttatt
    38281 cttcctatgt ggatgaactt tcttttaaga acatatgcat gggttgctat attaggaaaa
    38341 aatggacttt taaatacatt tttaggttgg tttggaatac aaccattagc aattttgtat
    38401 actaattttg ctatactttt aggtatggtg tataactttt taccatttat ggttcttcca
    38461 atatatacag cactatcaaa gatggataat gatttgataa acgctgctca tgacttgggt
    38521 gcaaataata tgacagtttt tagaaaaatt atatttcctt tgagtttgcc aggtgtaatg
    38581 tctggaataa ctatggtatt tatgccagca gtcactactt ttgcaatatc aagactgtta
    38641 ggtggaggta aaataatgct ggttggagat ttgattgaac aacagtttac agttgttgga
    38701 gattggaact ttggttcagc tatatcgata tttatgatga tagtaatatt aatatctatg
    38761 tctataatgt caaaatttgg agatgaatct gacaaagaag gcggtgggtt actattttaa
    38821 aattaaagaa aataacttct aatatatatt tagcattagt attcttattt ttgtatgcac
    38881 ccatatttgc tttggtacta ttttctttca atgattcaaa atcaatggca agatggaatg
    38941 gatttacatg gaagtggtat ggtcaattac ttcaaaatga gagtataatg agtgctttat
    39001 attatactat tgtaatagct atattagcgt ctgtaatatc tacaatagta ggaactataa
    39061 gtgctatagg aatacataag atgagaggta agagtaagaa attaatacta aatgtaaact
    39121 atcttcctat tttaaataca gagatagtta cagcagtagc tcttatgagc ctgtttgtat
    39181 ttgtaaaaat ggaatttgga tttacaacaa tgctattggc acatataatg ttttgtttgc
    39241 catacgttat actttcagtt ttgccaaaaa tgaagcaatt acctgataat atagaagatg
    39301 cagctatgga cttgggagca acacctatat acgctcttag aaaagtaatt ttaccacaaa
    39361 taaaaccagg tatagtttct ggatttttaa ttgcatttac tatgtcaata gatgacttta
    39421 taataagttt ctttaatgct ggaaatggtg taagcaatct ttctatagaa atctatggaa
    39481 tggctagaag aggtataaaa cctgaaataa atgcattatc aacaataatg tttgcagtgg
    39541 ttctaggttt acttttacta gctaataaaa aagaatctat agtaaggggg attaaataat
    39601 gattaagttt aaaaaaacaa tatcccttat agctgtaggt atgatgacag ttactttatt
    39661 tacaggatgt aataaagcaa ctgattctac aaaagtttta aatgtatata atgttggaga
    39721 ttatatagat gagtctctta tagataagtt tgaaaaagaa acaggaatag atgttcaata
    39781 ttcaacttat gatactaatg aaatgatgta tcaaaaagta aagagtggta gtacacacta
    39841 tgatttagta tttccttcag actatatggt agagaaaatg aaaaatgaag gtcttttgga
    39901 aaaattagat tttaagaata tacctaatat gaaatattta gataagagtt ttttaaatcc
    39961 tgtatacgac aaaactaatg aatatagtgt tgcatatatg tggggaactc ttggaatatt
    40021 gtacaataag aaagaagtta aagaccctat ggatagctgg aatatacttt ggaatccaaa
    40081 gtataaggga aatataatga tgtttgactc tgttagagat actataggga taactcttaa
    40141 aaaattagga tatagcatga atagtgtaaa tccaaaagaa ataaatgaag ctaaaaatct
    40201 tttaatgaaa caaaaggatt tagtattggc ttatgtaaat gatgaaggta aggatagatt
    40261 gcttggtgga gaagtggcaa tgggtatgct gtattctgga gatgcagtta cgctaatgga
    40321 gcaaaatcct gaccttgatt atgctatacc taaagaggga actaataaat gggtagatgc
    40381 tatgtgtgta cctacaacag cacaaaataa gaaagaagct gagctgttta taaacttttt
    40441 attagaccca gagaatgcaa aagtaaatgc tgagtacatt gggtattcaa cacctaatac
    40501 aggagcacta aaattattag acccagaaat aactcagaat cctgttgcat atccatctaa
    40561 agaagtactt gataaatgtg aaacttttga agatattggt gaaaatataa aattatatga
    40621 caaggcttgg atagagttaa aatcaaaata gggtcttgtt ggtttaagta aatttactta
    40681 ataaatactt aataaaactg ttctttttta gaaaatttat tttctaatta ttgaacagtt
    40741 ttattttttg tttaggtcat gtttttattt tgagtttatt tttatgagaa tttaaagtac
    40801 agtaagtttc attgattact atttttataa taataactat aaagaggttg tctcgaaatt
    40861 gagataagcc tctttttcat tgttgaaatg atgaaaacat gtttgatttc ataaaaaaag
    40921 agtgattcat gagctaaaga atttgctttg agacagtatt ttttattaag ttatattcat
    40981 atgcaattta aatagtttat taataaatga aaacttattt gtataatgat tttaataata
    41041 attagaaaaa atgggcaact taatagaata attggaggtg taaatttata aaaaatgaat
    41101 aggataaata aaaaaataga tttattaata ttggctttgc tcgtttttat atttttaatt
    41161 gttggaatag taatactatc gattaggaca gaaaataaat tgaatgtgtt cataatgtta
    41221 gcaataattt tttttataat aatgttaact tatttatcaa attcagtagt ggggttgata
    41281 acaagttcaa taattatatt tatgtataca tcctatattt tatataacaa tattactcac
    41341 aatatggatg ttgaatttat atcatatatg tggataatag caacaccagt atcttcaatt
    41401 ataatgggta atctgaacaa aagcataaat gaattgcaaa atacaaataa aaaactatct
    41461 gaacagtata aggaattagt gactattgat agtgaaactg gacttagaaa tttaaagatt
    41521 ttttataatg atgtaaatat ggagataagt aaatctatta gacataatac tgatttttct
    41581 cttatgatag taaaattacc atactacgga aacctacaga ctatatttgg tgaaaacaaa
    41641 actaataaaa tagtaaaata tattggttca aatattatag aatgtacaag aaatgaagat
    41701 ataatttata gtttacaaaa agatatgata gggatactaa tgccaaatac atctttggaa
    41761 ggttcgaaag ttgttaaaga cagaataaag aaaaggataa aagaattaaa tttagattta
    41821 aacaataggg gaaaatatgt aaatatagat gtcaaaattg catttttaca atataagagt
    41881 agttttggag atagtataaa ttttaagaat atagttgaag aggaacttca atatgatgtt
    41941 taaaatattt aataaaagtt tattaaaatt agtaattgta ttatcaatag tactgacaat
    42001 atcaaatttt agcttatttt catatgcatc agaaattact tccagcacag ataaggtatt
    42061 aatcatatat gattctaaga aagaaacagc ttataataga gatattttaa atattatgag
    42121 aactctattg ggaagattta gttcagatat agagctatta aaattgagta actatgatgg
    42181 tgagataaat gaaaattatt acagtcatat atttatttta ggaatcaatg aaaactcata
    42241 taataatgat aaaaacacta aaaacttgat tagttcctta aattcatata aagtaactat
    42301 atgttggtta ggatatggta tagaaaatct tttggaacat aaaaaataca atttagacta
    42361 tgtaggaaaa acaaacaata tagttagtgt aaattataga ggtaaatcat ataacttgga
    42421 tgaacattat gtttttaaca ttgtagaatc aaaagataca agtaataaag taataggaag
    42481 tattaatgat accttaaata agtatccata tataataaat gataaaaatc ttttttatgt
    42541 atcaaaatta gatttagatg gagttttatt ttatatattt tgcgattcac ttaatgatat
    42601 ttttaatatt aagacgtttg ataaaggacg tatatttgta agaatagaag atgtacatgc
    42661 ttttagagaa ccaaaaaatc ttgtagagat agcggattat ttaagtagta agaatatacc
    42721 atttacgatt gcattaatac cagcatatgt aaatcctaaa aatcataaag taataacttt
    42781 atcagaaagc cctgaaattg tcaaagcaat aaaatatatg caagataaag gtggaactgt
    42841 gatattgcat ggatatactc atcaatacaa gaaagaagaa gtttctggag aagggtatga
    42901 attttgggat ggtaaaaaag atgaaccctt aaaagaaaat atgaagatat ttgtcaaaga
    42961 tagagttttg aatggtctta gagtatgtat agagaatgga atttatcctc ttgcatttga
    43021 agcgccacat tatgcaatgg aatcagaggg atataaagaa ttaaaaaaat atttttctac
    43081 ttatatgggg caacatcaaa ataatgataa aaaatttagc acaaatacat atccttatat
    43141 tattagggat acagaggagt ttaatatatt tatacccgaa aatttaggat atatagaccc
    43201 agaagacaag tttacttttc aaaatataaa agaaaattta gataaattat ctattgtaag
    43261 aggttttagt ggagggttct tctttcattc atatttaaac atagagtatc taaaaaacac
    43321 aatagaatat ttagaaaaac agaatataga atttatgaat ttaagagatt ttaataattg
    43381 ggtaaaagtt gatgaaatac aaataagaaa taatggagat gaaattatag tcaactatga
    43441 taaggactta gatgaaataa ctaaaagtga tactagattt aaatacatat ccaatattag
    43501 taaaatttta atattcatag tatctataag tgttttaata tttgtgatta tatttatata
    43561 ttttaagaga atagataaaa agaaattttt aaaataaatg attttatatt agaatcaata
    43621 ttttaaaata ttttaggaga aatttatgag agatttaaat gtatcagagt atttgttcgt
    43681 atttagtctg tttagtatat ggtctttatt gcttataaat attattttag ctatgggtgg
    43741 atatatattt tattttaaaa actttgataa agaaataaaa gaaattgatg aatatcctat
    43801 gatatcaata cttgttcctg ctcataatga agctaaagtg ataggtagaa ctgtggagtc
    43861 tttgttatta ctcaattatc ctaagagtaa gatggaactt attgttataa atgacaattc
    43921 ttctgataat agtaaagaaa ttttagaaaa tataaaggat aggtacaaca attataattt
    43981 cactataata aatacagata gtttaacagg tggaaaagga aagtctaatg cattaaatat
    44041 tggatataag gtttcaaaag gagattttat agctgtttat gatgcagata acacaccaga
    44101 taaaaatgca cttagatatc tagtacaaac tatagtaatg aatgatgaat taggtgctgt
    44161 tattgggaaa tttaggactc gaaataaaaa taaaaaccta ttaaccaaat ttataaacat
    44221 agaaacatta agctttcaat ggatgtcaca agctggtaga tggcaacttt ttaatctatg
    44281 tacaatacca ggaactaact ttatattgag gagaagtatc atagaagaaa ttgaaggatg
    44341 ggatagtaaa gcaattgcag aagatacaga aataagtttt aggatatata aattgggata
    44401 caagataaag cttgtaccac aatctataac atgggaacaa gagccagaaa ctgttaaggt
    44461 gtggataaag caacgtacta gatgggcaaa agggaatata tatgtattaa tgaaatatat
    44521 aaaaaatata tttaaacagg gaagaaataa gattgtgttt gatatagctt actttttttc
    44581 tgtatatttt ctatttttaa caactgtaat tatatccgat attttatgtg ttttaagtat
    44641 atctaaacta gtagaaattt ctattccaat taactttttt ctaatatgga tactatctta
    44701 tcttttattt attatagaag tgagtataag ccttactata gaaaaaggtg aagcaacaat
    44761 tgaaaatata ttcatagttg caataatgta ttttacatac tctcaactat ggctgtttgt
    44821 agctataaaa gggatgatag agtatctaaa agatataatt tttaaaagag aggttaaatg
    44881 gtataaaaca gaaagatttt gattcatgta caggaggaat taacatgaaa aaatttatta
    44941 tatcaattat atctttagta ttgttttttt caaatatatc acttatatac aaagtaaatg
    45001 ctgatgagac taaagttaaa aattataagt ttgaaagaga tataactatt gatggtgtta
    45061 taggaagtaa tagtactttt tttgaagtaa ataaaaattg ggatattgaa gaagttttat
    45121 tgcatcttaa ttttagtaag agccaaatat taaatggaga tgtttcaagt ttaactgtat
    45181 tgataaataa tgtaccaata aaatctatta aattaaatgc taagacaaat tataaaaata
    45241 cattagaagt attagttcca aaagattata taattcaagg atataatgaa ataaaaataa
    45301 aaacatacaa aacaatatct gataaaattt gccaagatga ttccaatact gggaactgga
    45361 tggttattca caaagaatca tatatatcaa ttagatataa gcagaaaaag gtggaaaact
    45421 caataaatga atatccgtat ccatatgcag aaattgaaaa taatcataaa ttagatacaa
    45481 ctatagtagt accagataat atgactagag gagagactac agcagtgttt aatttggcat
    45541 catcatttgg aaaaataaca aaaaatgatg atttaaagtt agatgttaaa ttatattcag
    45601 aaatgaaaaa ttggtcagat gataatatta tatatattgg taaacctgaa aatacagcag
    45661 aagaaatttt ggatatttta agtataaagg aacaaaccct attatcttca aactgtataa
    45721 tcaagcaagt agattcacca tacaataaga ataagaagat gatggtggta attggtagta
    45781 atgaagatga tttaataaag gcatcaaatt tattaataga aaatagactt tctaaccaag
    45841 ttttatcatc atcagtgctt gtaaacaaag agactaatat aaaaattaat agggaacaaa
    45901 aattaaattt aggtcattta acattaaaag acttaggata ttctgacttt ttgttggaag
    45961 gagcatttaa tcaacaagct ttgtttgatg tcaaaatacc aacgggtaag gtattagatg
    46021 atggttcaaa gattatctta aatttaagat attctgacaa tttagatttt gaaaaatctt
    46081 tagttacagt gtctataaat gatgttattg tgggaagtaa aaaattggat aggtcacatt
    46141 ctaataatga taagttagaa ttaaaaatac ctaaagatat agataataaa aactattatc
    46201 aagttaaatt gacatttaat ttgagtataa aaaattcgaa ttgtgttact agagagagta
    46261 ataatccttg ggcatatgta tcaaataatt cttatttggc gttatctacg aaagaaaatg
    46321 aaaccttatc ttttgaaaat tatccgtatc catttgttag agatgatgag tttaatgatt
    46381 taactgtgat aatgccagat tattcagggt cacaagctat gacctggatg tttagattag
    46441 gtgttacttt aggtacgaat ataaattcac ataatggaaa tataaatgta attagaggaa
    46501 aagaatttag tgacaagtat aaggatacta atattgtagt ttttggagtg ccacataata
    46561 attctgttat aaagatgtta aataataatt taaatataaa gttcgataaa aattactcca
    46621 attttatatc aaatgataag ataagcttta ttgatgatta tggaaaaaat atttctacca
    46681 ttcaattaat aaaatcacca tataataatc aaaaaaatat catggtaatt agttcaatga
    46741 atgaaaaaaa cttatattta ggaatggatt atttgttgaa taaaagtaaa gttaatgatt
    46801 taaaaggaga tacattgata attgatgaat acggagaggt agaagattta gcttataatt
    46861 taaaacctaa gaaagaagta aaagattcaa gctggaatat gagtataaat aagacaacta
    46921 aagtattttt aatgatttct tttattacta taattgtagt catgatatta tcaatgctat
    46981 atataaaaaa atataaaaga agataatatg ataaatttat tattttaaaa gaggtatata
    47041 atttgaggaa atatatgaat aaatttaaat taaaaagaag cattttaatc tcattaataa
    47101 tcttaatttt tatatttata gtgtactttg taagtattga gttaaaagat gggagtctag
    47161 taaataatca atataaaggt aagattaaat cggccaattt atctgtagat tatgaaataa
    47221 atcaagttat gaaggatata gataaattgg gattgaatac agtaaatgta cctataatta
    47281 taaatgtaga gtccattaat tctgatatta tgtcaataga taataatagt aaagaaaagg
    47341 caattaagtt aataaaaaaa ttaaataaaa aaggtatttc aacaatatta gaggcatatc
    47401 catgggttga aaatggaaaa ttgtatgaga ctgactggaa ccctataaat aaaaaaagat
    47461 ttttttatac ttggcaaaat gccatattaa atgaacttat aattgatgta gcaaatccac
    47521 tggatgtaaa tgttttaaat attggttcta attttgtaca tttggaagaa tatcaacaaa
    47581 attggggtga aataatagat tttgtacaat ccaaatttga tggtcttgta acgtacagaa
    47641 ctaattggtg gtatacaaaa aaagatgatt caagtagtaa gttgttttat gaacaaaaat
    47701 tgaacaataa tttttttgat aagttagatt ttatatctat agcagcatat tttgagttaa
    47761 gtaataaacc tgttaataca gtggatgaat taatttctgc cttacactca tctactgtaa
    47821 acaatagagg acaaaatata aaacaagaaa tatataacct ttataaagct catagaaaac
    47881 ctatattttt tggagaatta ggtttttcaa atagagaaag tgcaagttct cagccgtgga
    47941 atcatactcc ttcaaaagct gtaaatggcg aagaacaagc aaggtgtttt gaggcatata
    48001 agaaagtttt tgaaaatgaa gactggataa atggtttttc tgtgttttgt gtaggtaaaa
    48061 tagatgacga aaaaaatttt tacccatcaa aagagagtat aaaagttatt aagtcttggt
    48121 atgaataatt ttaaaagtgt agaatttata tagattaaat agatgattag gagagatata
    48181 aatgaatgac atcaaggtta tgactgtatt tggaactaga cctgaagcta tcaaagtagc
    48241 acctttaata aaggaacttg aaaaaagaga gaatataaaa agtattgttt gcgttacagc
    48301 tcaacataga gaaatgcttg accaagttat agaaaccttc aatataaatg tagactatga
    48361 tttggatata atggaaaaag gacaaagcct gaatgatata acatgtaaga ttttaaataa
    48421 acttcccttg attttaaaca aggaaaatcc aaatataata ttggtacatg gagatacaac
    48481 cacaactcta gctacaagtt tgactgcatt ttataataaa gctcttgttg gtcatataga
    48541 agctgggctt agaacctatg ataaatattc tccttttcca gaggagttaa atagacaact
    48601 tacaggtatt attgctgata tgcactttgc tcctactaat ttggctagaa aaaacttgat
    48661 ttctgaagga aaacctaata ataatatatt tgtaactggt aatactgcta ttgatgcttt
    48721 aaagatgaca ataaaagaaa actataatca tcctataatt gatgagattg gtaacgatag
    48781 gatgattttg ttaacctctc acagaagaga aaatttgggt aaacctatga aaaatatatt
    48841 cagagctata aagcgaatag tagatgattt tgaagatgta cagatagtat atcccataca
    48901 tttaaatcct aaaatacgaa ctatagctga tgagatattt ggaaaatttc ctgaaaaaat
    48961 tcatattata gagcctttag atgtagctga ttttcataac tttttaaata aatcttatat
    49021 gataatgact gatagtggag gaatacagga agaagctcca tcattaggta agccagtcct
    49081 tgtgttgcgt gataaaacag aaagaacaga aggaattgaa gcaaagacac ttaaattagt
    49141 aggtactaat gaagatagaa tatataattc agtgagcgat ttattaataa ataaagatag
    49201 ctatgttcag atgagtaaag catctaatcc atatggagat ggaaatgcaa gcaaatatat
    49261 agtggatata ataattaaaa aatttaattg caaatattta aattagataa ggtctaaaac
    49321 attaactaat tgatataaga tttatgataa tattaaggaa tacaattttt aagggggagt
    49381 aatatggcta gaaaattaat aaaaaattta ggtaaaagta aaagtgtaaa gagagtgaaa
    49441 cttttattta agaagatttt tattactgta ttcatagtag caagtatagt tgctattttt
    49501 aatataacga aatattttga ggaattatac aaagtaaggg atttaaaaag tactaaaatt
    49561 gaatactata tggatgtggc tgatgaagca ggagatggaa aggtccaatt aagttggaag
    49621 gccttacttg ctatagacat ggtaatacat gatgaagatt taagtaatat aaaaaagaaa
    49681 gacacattgg atatagggga aaagtttata gtagaagata aaaatgataa aggcgaaaag
    49741 gtgtataagg taaaaaagtt taataaggta ttaagcgaat tgaaatttga ctcttctcaa
    49801 aaaagtagag caagaaaata catgaaagat ttggaataca cataccttgg aaataaacaa
    49861 ttagatagta gtgatgaaaa aattaaattt ataaagaagt tagaagactc agctataaga
    49921 gaatatattg attatggaat attgccctct gtaacaattg gacaagctat attagaatct
    49981 ggctggggaa attctaaact tacaaaacag agtaataatt tatttggtat aaaagcagat
    50041 aaagcatgga aaggaaaaag tgtagaaatt tcaacttcag agcattataa tgaaaaaatt
    50101 gtagctagtt ttaggtcgta taattcatta caagattctg tcaaagacca cagtttattt
    50161 ttaattaata ataaaagata tagaaaacat gggttgtttg aggcaaaaga ttatattagt
    50221 caagctcaag cattagaaaa tgcaggatat agtacagcag aagataaaaa aggaaatcgt
    50281 atatatgcag agctattaat agatattatt aggagttata atttgcaatt gatagacaat
    50341 aaagttgaga caaaataatt gttaacttta tatgaggaga taacaagata agattctttt
    50401 ctacaaaaag aatagaaaaa ttgtaaaaaa aactaaaaaa atgtattgac ttgttattta
    50461 aaaggtgata ttattattac tgtactcgaa agagaacaaa a
//
LOCUS       DAELXX010000014        50437 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.014, whole genome shotgun sequence.
ACCESSION   DAELXX010000014 DAELXX010000000
VERSION     DAELXX010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 50437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50437
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.014"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..791
                     /locus_tag="KPH29_000804"
     CDS             <1..791
                     /locus_tag="KPH29_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466434.1"
                     /translation="EINYKICDKLSIDYYRKNLVHYKNNRVAIMDLNSYLIKKSDNLS
                     KKISINERSYEIFKDEKFISSKVGKEVLKNLKIDLIKDLNVYKTPEPFIYLSINRISP
                     QKILIIENKDTYITITKMLLEGKEILKNKIDTVIYGEGKKIINSIKGLKEDITIEYLT
                     NKENEFLYWGDIDKTGLSIYTSLKDTNEINNIRLFEVAYEHMIDKTKENTLRNIPKNQ
                     KDNFECGLENINNLNLKESIKNILVQGKYIPQEALNRFDLEEYN"
     gene            791..2410
                     /locus_tag="KPH29_000805"
     CDS             791..2410
                     /locus_tag="KPH29_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466435.1"
                     /translation="MEMINILEGYNKRVNCIGSYYLIATLANNRNKFKEFDNIQFYNL
                     LIQVLCYIFDRSLRRKNCLRDDIKDFIEEINRMDYKIMLSEDDLKDLANYIINGLTNS
                     GKVYLFTYYSLEQEKHIDESIKIIEDKNVKINNQERLSYSLTTEGYRLLLSTKEYDEL
                     FQIQISQMIAKLRIEKGDYQGAKEDIYDIINTLEIHQQKIDNYIKIIKVNILYIKEEK
                     YSTVIDTTVETLLEEMDKYEELKKEVKLLIKDKEDYLEKDEDIDKEKIKNIRTQLLQM
                     KELIDGIVLAKSTATNLLSRVQDFREEFKEILKKLLTTPVIKRFNFKEEILDRLEKDS
                     DFLLIIRELYISLFKVELPNRFNIGVAYQEQKLLNTREIENEIVNKDELEIIENKEKE
                     ERINYCENYYFDFFKYLIKYGLEKGQFTLEEFLKNYLKYDKVIYLEITKDSKLMRDII
                     IYFANQRESINIKKIIRMLENSAFSPNTDFQIERLIERLLVEDFELLNSVSHIKSNKI
                     FEDEMKINTEINLETMTAVKLEIANIKFEII"
     gene            2428..3156
                     /locus_tag="KPH29_000806"
     CDS             2428..3156
                     /locus_tag="KPH29_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466436.1"
                     /translation="MNISLNDYKIANEIFYRLLKKSELKEKEAKELFLAYINNSNVRY
                     ALEAICEVSKIKIILISDTLYLIPNNDNDIFGFDYRRESLLGKDLKDSYLGYLIMTII
                     FSEFTNGVAPASYIQVLNLLDLVQESLERSIARDNLEEIEKSCEFNITILKNYWESKR
                     KWDESSKKGIETTSADFQIGFIRKIINFLKNEGLIVHIEDEDKIKPTRRFLDLMNNYF
                     LDEERKVLIEKLVLRKGESINASN"
     gene            3143..7498
                     /locus_tag="KPH29_000807"
     CDS             3143..7498
                     /locus_tag="KPH29_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466437.1"
                     /translation="MPVINKYKIINFKFGMGQERILSNRTMELYGENLQVEAENTAGK
                     TMSIQTIIQSICPLSDVAKPFTAIYNKKEPLYSMTEWLLDDGKTLLLTGIGFEKKAGS
                     SKEEGKEKKDDLYKYFMFIIEESKEMEVDIDNIPLLKNNDKGIRIVQPLTASQDYIKM
                     LRDKYGKNKVHFFNNYLRKQHREKLEEYGINQSEWKEIIIKLNSNNKEGGLSDFVKEH
                     NTSEKLIRDKILPLIETSLTTEEFAPILQIKEQVRKCINNIIDMNEKLSDYKNYRDLN
                     IRVESLQNKINRILFIEEDKNNVLGELANLYVHIENKRNELNEEIKQYQKMKASKEEE
                     FKIVEYEEDSYEYFRKEKEIQDTQVEFDKAKNELNDKIEELELDEQYLNISKFKRKKK
                     GLEEGEKDKIEKESEKQRKEVNQEEITAIINRLGSSIHYCLKEESIINNEKLKENTNN
                     NIAIQEKKNQLNDDKSKLTKVLISKNSEIKYIEGKERDFNNQLHEFKLNNTDIKGFIS
                     NNFLGEVIDYTRYINYLNIKVKSIEENLESKVMQIEESNKLKDDINEAINNLKLDNHE
                     SYMKLQVLKTRKKDIDNFKEKYKIIKTRFEIDNELDNSTKLYKEVKQELNKMKLQVQD
                     LENNKKQLEKELEDLQNYKDIIFEEEILTDLERNGIKPIEGMKYLLTLDGDLEYKNNL
                     ISKNPLLPYSIIITNKEYQKLKQINLKNKTKISMPIFVRDNIEDTFITTSSNLVISNG
                     LNILSSFDNKALDPEKREKDMNLIKEQINSIRDKVEQYKAEIEILEEFKLLTNKIDFT
                     AEDLSIDVKIENYKKIIFEINNKIKSNENNEKNLSNKIKELYENKEKLLNNKNILFNK
                     LKDIRNIQDTYSIILENKNEITKLKTSIEKIEEEKKVIKKSIEDIDVNIFNLNSEKDR
                     INRHIDDTESKINKFKIYEPIKLENAKLEELEIKYKKYLEDPTKKEIETLNYEISVCL
                     KRIESITIELEEFRQLISSDNFENIPILFTIEELNKRIVLNKSKVDELRKLKYSIKSD
                     IDKNQGSLNTKREEILKKFNGKEPLSTSLIVNFNFEERKIDIKKDIKDIGEIIEGNRT
                     TCQNLEQELRDLKKYKVERKAITYQPEDIFIESKKLQSKLQNVEYNLIKSINSYKNTI
                     LEIKEFNYKHMEKYKNFIETLNVNKDNYSRQKEQVEIVLLVIREEISKLEKHSEQLRK
                     EKTIISRQIKDYVMDCIDEFKIINKLGKHKGQALFNISLPKSDKIENSLSLVDMLMDE
                     IVEEANLGDVEQKINTFYILNKTLNIGRIPVSVIKYEINRNETIKWNSINNETTGGQR
                     FCISFIVTILLMEYKRYDRNAIVDESKYKGKVLLMDNPFGETSQQDFLKEIFELAKKF
                     NVQIISYTHVTNASIRAMFNKIYLMTVEKTTSNKEFVDINEIKQNKTDELVYMSKFYI
                     GNKEELIQEDIFSLI"
     gene            7600..7809
                     /locus_tag="KPH29_000808"
     CDS             7600..7809
                     /locus_tag="KPH29_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466438.1"
                     /translation="MKNFVIVSTKAALLRVIIVRLLLKIGLKTYKYLSYFMKKEKLKR
                     IIRPEGCELRVNRSIQVERAIAEQI"
     gene            8205..8345
                     /locus_tag="KPH29_000809"
     CDS             8205..8345
                     /locus_tag="KPH29_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1466439.1"
                     /translation="MKIKIKKYREKLLMIQNELAKSVGVRRETIVHLENGLMYHKGIQ
                     DK"
     gene            8483..9445
                     /locus_tag="KPH29_000810"
     CDS             8483..9445
                     /locus_tag="KPH29_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017561613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBG1466440.1"
                     /translation="MAKIDNMMSILWMLNSDKKVTAKQISEKLEINIRTVYRHIDALS
                     ASGVPIISDTGHNGGYTLLNNFIKAPLFFDTEEQTALLHAAIFAKEEGSFLDDTLDKA
                     TSKLKMYSNQKQEETLTKHLIGFEVIKPTGKISIESVLKKLKNSIANELSVEINYRTA
                     HEEKSKCRIINPYGIVRWNNNWYIVAFCQMRNEIRSFRVDRISSVIETSNTFNRPKEF
                     SASEFFIRGIMDETKDEDNLISLVIEGKMNALDNLCQHWLLGYYLKERTSNKAIFKLQ
                     EEIIFTYVPNILIPYRKSIQIIEPLSLRDKMVEDLLELINHYQI"
     gene            9523..10152
                     /locus_tag="KPH29_000811"
     CDS             9523..10152
                     /locus_tag="KPH29_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBG1466441.1"
                     /translation="MKKRKVYMYVFDTMSDWEVGYLTAELNTGRYFKKGLKPLKVITV
                     GVDKNPIITMGGLKVLPEITIDELNIESNDLLILPGGNTWTSIGHEKILEKSKEALEQ
                     RSIVAAICGATLGLAKTGLLDFRNHTSNDLEFLKMVILSYSGEKYYKMESAVNDENLI
                     TASGIAPLEFTVQVLKVLDVFKEEALSSWLNLYKTHDSKYFFELMNCNK"
     gene            10983..11399
                     /locus_tag="KPH29_000812"
     CDS             10983..11399
                     /locus_tag="KPH29_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466442.1"
                     /translation="MSKSETGVKSKLILELLVITYLFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSKFILSKVNQIAITIMMYSIVIIAIFFATPYISNI
                     NISNLDTGIILIAVLFIQSLSKLILYIYFDKRGICN"
     gene            11400..11612
                     /locus_tag="KPH29_000813"
     CDS             11400..11612
                     /locus_tag="KPH29_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1466443.1"
                     /translation="MAISMKTKIKEYREKLLMTQNELAKSVGVRRETIVHLENGKYNP
                     SLKLAMDIAKVFGTTVENLFEFIEED"
     gene            12247..13290
                     /locus_tag="KPH29_000814"
     CDS             12247..13290
                     /locus_tag="KPH29_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBG1466444.1"
                     /translation="MKRKIAVVLLTVAMLLSLVACSNDNTSKSDKLEDTLVVYTTHGE
                     DMLGAISEEFTKETGVNVEFINLKGELADRVRSEKDNPQADIMFGGDTSTYMQLKEEG
                     LYEKTEPSWNNELDKSYKDSDGYWYGTIKTPVMMFYNTKLLSADKAPKDWSDLTKEEY
                     KDLIVTRDSLSSSMRSTISNLINYYSNTKSEEEAWKYLEDLNKNIKNYYNSGSVMYSA
                     IGKGEATISVAVLSDIIENKEKNNMPLEVVDAESGSVVITDCVAALKNAPHPNAAAKF
                     VDFVGSAKVQAMVANDFNRIPTLDSALKDSPKWMSKEYKAMDVDWSVIGKNQSNWIEK
                     WETDIIDNNKTIK"
     gene            13551..14425
                     /locus_tag="KPH29_000815"
                     /pseudo
     CDS             13551..14425
                     /locus_tag="KPH29_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421888.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14444..15436
                     /locus_tag="KPH29_000816"
     CDS             14444..15436
                     /locus_tag="KPH29_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1466445.1"
                     /translation="MKNIELKEINHYYGKEKVLHDINISIKEGEFFTLLGPSGCGKTT
                     ILRIIGGFIKPSSGSIYVGDKNITNLEPENRNMGTVFQNYALFPNMTVEENVSYGLKI
                     KKLSKKIIKEKCDNYLELSGMKDFRKKKIDELSGGQQQRVAVARSLATEPTMLLLDEP
                     MSNLDIALRIKMREEIREIQQKTGITTLFITHDQQEALAISDRIAVMDKGKVLQIGVP
                     IEVYKSPLNDFVANFVGTSNCIGKEDYVNFNIEKETKPYIYKRPEEMILLQNTNQSGF
                     VKVKVESKNFLGAILEYTVSNDGKKYQVTELNRLNNSNKLNVGDMAYLGVLQGD"
     gene            15438..17132
                     /locus_tag="KPH29_000817"
     CDS             15438..17132
                     /locus_tag="KPH29_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBG1466446.1"
                     /translation="MIKKINRSKFTSKNVIFIVLYSILFWILIGYIIIPIGNTFVQAF
                     QGKGGYSFEVFKEYFSNTNNLRVVSNTIILGLGSVFVCGIMGISLALYMTFVCDKHKK
                     IIHILLLSPMMIPGVIMVIACIQIYGESGIVTKSIEILLPFIKKPYDFSGLKGILFVV
                     AYTQYVYFYLNVYVALKYVDYSTVEAAKGMGASKLRVFFDVIWPVITPAVITSTIITF
                     ASGISSFSAPNLIGGGFKVLSTQIVRSKANNHMDIASVQVITLLLMGILFMILLQYYG
                     KKYGVVPSLKSQSYKVHNRKKDLFTFVCKVIIEIQIIMIMLPIVTIVYLSFVSTHSIM
                     TTIFPRDFTLENYQMIFQKSRVLQPILNSFKMSLMAVAVGLIITVPVSYLVIKKDTAY
                     NRLAKFIIMLPWSMPASAIAVNLINTFNQKSIFAFNESLIGGFYIVPIAYTIYALPLL
                     FSSNEVAMKSINLGLEESSRSLGAGRIKTFYNVIVPNMMPGIISGAILVFIRTIGEYT
                     MSALLYGVYNRPISISIVTNMQEFKIGVSMAYGVLVIGICYIALGIIFKLDRKRFT"
     gene            17683..18561
                     /locus_tag="KPH29_000818"
     CDS             17683..18561
                     /locus_tag="KPH29_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBG1466447.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQGANEKNAPV
                     ILAIHPNELSFLGDSFVVSCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVDTLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKAAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            18597..20492
                     /locus_tag="KPH29_000819"
     CDS             18597..20492
                     /locus_tag="KPH29_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG1466448.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKNLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSETKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFPPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVAKPTIRSENS
                     EVELLGA"
     gene            20760..22667
                     /locus_tag="KPH29_000820"
     CDS             20760..22667
                     /locus_tag="KPH29_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG1466449.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISELTRLDSATFSALINLFDIDSIIFVETIITELEYNLG
                     YTINQPYYINLITHILICLKRIEEGNQIESQEEREISVDNLDELVYKNVTTLIDKIEN
                     RYRVKITKDEIMYIYIFLVSSGFSSENNSSYNDESDILSESEKVSSIMIENMSQFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSKRNLDDVDFVISTVNLDLKDKLHIVVSVLLMESDINNIKNALLSKPKK
                     SMNLDTNLLGMYLEDNIYFNFDVAQMESLIGVNLGSKYESISLGKDLNIYIHKQSKLN
                     KGIMNINNVTRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKSKEDIKILF
                     KNNLLEKTNGN"
     gene            22657..23106
                     /locus_tag="KPH29_000821"
     CDS             22657..23106
                     /locus_tag="KPH29_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG1466450.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASIVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNISERLLNVHTKKEILDIFYEV"
     gene            23206..24093
                     /locus_tag="KPH29_000822"
     CDS             23206..24093
                     /locus_tag="KPH29_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG1466451.1"
                     /translation="MAIYLEMPKLIHEFPFRSLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVS
                     PLEFKKRYNSLEKKGGASK"
     gene            24498..25946
                     /locus_tag="KPH29_000823"
     CDS             24498..25946
                     /locus_tag="KPH29_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBG1466452.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKD
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIIHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERHNLFFSDVQVRGVYPSF
                     MNYYLKSNQIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMILGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            25972..28362
                     /locus_tag="KPH29_000824"
     CDS             25972..28362
                     /locus_tag="KPH29_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBG1466453.1"
                     /translation="MSLIFTTKDQLIEQGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDCFD
                     LSKGITSEHTRILDLNEGIVKRSFIWENSRGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMEKFNCEKVDDPRVASINKKQFKINVKKYNNKYFMNLVTERS
                     RLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSAGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSILDASRERARELGINKGCLYSWRTINGNECSAYYPAGTAQ
                     FHINADIAYGIRTYFEATNDEEFMMQNGLEILIETARFWVEFGDYIELKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKDWI
                     NAAQNIYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLNYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKKDKLSFEPRIPKHWKNI
                     KFNILFRDNLVSVNIEEYKSYYKLIKGKNISILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            28364..29035
                     /gene="pgmB"
                     /locus_tag="KPH29_000825"
     CDS             28364..29035
                     /gene="pgmB"
                     /locus_tag="KPH29_000825"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBG1466454.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     ISREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGIKDEFDCIVNPSLLERGKPYPDIFIEGSKMLNL
                     KQYDCVGIEDSYSGIKAINAANMTSIGVGKKEVLGKCDHIVEDTSLLNYNTLLQAWSK
                     KINLD"
     gene            29074..30474
                     /locus_tag="KPH29_000826"
     CDS             29074..30474
                     /locus_tag="KPH29_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG1466455.1"
                     /translation="MKNKYEEVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINTTGDIKSDIKNENKKNSI
                     LNNIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYF
                     MPIILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGDPISFIGVPIQGVIYAASV
                     IPALLTVWMMSYIEKFINKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLA
                     SIINLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCA
                     NLAQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGA
                     GIAGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFG
                     VEKNVKESTDNVVEEV"
     gene            30791..31633
                     /locus_tag="KPH29_000827"
     CDS             30791..31633
                     /locus_tag="KPH29_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG1466456.1"
                     /translation="MIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLD
                     SAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELIYLTIHLAKVVK
                     DSNM"
     gene            31778..33706
                     /locus_tag="KPH29_000828"
     CDS             31778..33706
                     /locus_tag="KPH29_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG1466457.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKFVPDLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMTIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVASPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVDAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            33767..35194
                     /gene="ascB"
                     /locus_tag="KPH29_000829"
     CDS             33767..35194
                     /gene="ascB"
                     /locus_tag="KPH29_000829"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBG1466458.1"
                     /translation="MSFKDDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYRGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLVGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFIVENGLGAKDIADENGYVEDDYR
                     INYLREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
     gene            35605..36381
                     /locus_tag="KPH29_000830"
     CDS             35605..36381
                     /locus_tag="KPH29_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG1466459.1"
                     /translation="MKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQDCL
                     MCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVSSE
                     IVKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNSPK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            36397..36762
                     /locus_tag="KPH29_000831"
     CDS             36397..36762
                     /locus_tag="KPH29_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG1466460.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITKKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWKSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(36928..37677)
                     /locus_tag="KPH29_000832"
     CDS             complement(36928..37677)
                     /locus_tag="KPH29_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBG1466461.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELISA
                     GKPFIFVCPTYAWRLPIVVTDFIKETKFLGDNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFETITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNS
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            complement(38122..38679)
                     /locus_tag="KPH29_000833"
     CDS             complement(38122..38679)
                     /locus_tag="KPH29_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG1466462.1"
                     /translation="MRTLKYAILGLLNRNPMTGYDIVKEFNFQLAEFWNAKHSQIYPE
                     LKKLVNEKLIVYDIKISGDVLEKKLYTITEKGKDEFLKWLEKDEPMERTPKDIFRLRM
                     YFSNNLDIDTRLILLEHQLLQHQERITFLYSQMERYSEVPPLDSNDFGDYIVLDGAII
                     REQGAIKWLTNCINHHKNLDNTTLK"
     gene            38931..40229
                     /locus_tag="KPH29_000834"
     CDS             38931..40229
                     /locus_tag="KPH29_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HBG1466463.1"
                     /translation="MNLTQKRWIILIASCFINLCIGSIYAWSVFASPMAEHLNSLAGT
                     TFTAASLAIVFTVTNSVGPITMITGGMINDKFGPKKVVFVGGLIFGIGMILAGFSKGL
                     PMLILSYGIITGLGVGMVYGCTISNSIKFFPDKSGLVGGITTAAYGFSSVIMPPVANW
                     IINKYGVTSAFKSIGLVFLIIVCVSSFFIKKCPADFTPEGWVPPEKKGKVRVVINKNW
                     KEMLSTPIFYLMIIILTCGAFYGLMCVSLASSLATNMIGMTSSDSAIVVSILALFNTL
                     GRIVAGYISDKIGRINTLSGALVISIFGLLCLLFSREGDVATFYIGIIIIGICFGSFM
                     GIFPGFTVDKFGPKNNSVNYGIMFIGFAFAGYIGPAVMKNVYSVDGNYQRAFIIAIII
                     ALIGFILTFLYKYINKIQNLKIENLIKGDIKNGKKISKSM"
     gene            40201..42150
                     /locus_tag="KPH29_000835"
     CDS             40201..42150
                     /locus_tag="KPH29_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015394831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG1466464.1"
                     /translation="MERKYPNLCKPIKIGNVTFKNRMFSAPMGGTDITADCTIGSKST
                     AFYELRAKGGAGAVTISECMVHPETDGSHAYHLDLKIVDSLASFTYTADAIRRHGAIP
                     SVELSHSGQYSGTYLVDKDKKRGLSQWGVSPSVRPDGLEIKELTEEIIAEIVESYGNV
                     AALAKRAGFEMIMIHGGHGWLINQFLSPYFNKRTDKYGGSLENRVRFAKEVLDSVRKA
                     VGPGFPIEFRMSGSELFEGGYDLDYGTEIAKLLESRVDLLHVSAGTYQRGFAVTHPSM
                     FLEHGCNVYLAEEIKKHVNIPVATIGALNDPEMMEEIIASGKADVIYMARALLADHEL
                     PRKVMSNQDEKIVKCLRCFTCMAERATTSTRRCTVNPLIGRELDGTEVVSVLKPKKVL
                     IAGGGPGGLQAAITAVKRGHKVILCEKTNELGGILKGEQALPFKYKMYELGNTFGKIA
                     KDLGVEVRLNTTVTKEYVENENVDALIIAVGSEPLVPPIEGLDGENVVIVNDYYVEKE
                     KVTDEVIVLGGGLAGCEAAIHLAQEEKTVHLVEMRAELAPDANIRHRPILLEEIERQG
                     IYVYTEHKGLSVTTEGVVCMNKSGNKINIPGTSVICALGQKPRREVVDTLLDSAPYVA
                     QIGDCVRASTITNAVYQGHHAALDI"
     gene            42587..42724
                     /locus_tag="KPH29_000836"
     CDS             42587..42724
                     /locus_tag="KPH29_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1466465.1"
                     /translation="MINENLKSLRKIHQYTQEELAEKLNVSRQSIAKWESGVSQTKGY
                     Y"
     gene            42952..43326
                     /locus_tag="KPH29_000837"
     CDS             42952..43326
                     /locus_tag="KPH29_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HBG1466466.1"
                     /translation="MFNLIEMLFPIMFILIFIIIVFTFVKGIATWFKNNNSPRLTVSA
                     RIVAKRQNTTHNNQPNAGDISGAHGFHTISSTSYYVTFQVDSGDRIELSVSGSEYGVL
                     AEGDKGQLTFQGTRYLTFDREN"
     gene            43342..43989
                     /locus_tag="KPH29_000838"
     CDS             43342..43989
                     /locus_tag="KPH29_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466467.1"
                     /translation="MNKFLSQIRRIDNEISITRKIINTIFILCFGIVLGTFAKFLDTT
                     ASNTLPFIFEYLDISNFFGRFAIWLLIALYIAIYSHSSIRASLNVLVFFIGMVSSYYL
                     YSYFVAGFFPKNYAMIWLGFTVISPLLAFICWYAKGKSKISFILSVIIIAILFNFTFI
                     YGWIYFDVYSILEVIVFGCALIALKRNTFRETTYMILSAVVIAVILNMLVPFHFG"
     gene            44369..45448
                     /locus_tag="KPH29_000839"
     CDS             44369..45448
                     /locus_tag="KPH29_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG1466468.1"
                     /translation="MNDMKFIETLKQKRNVCDYSQSRLAQELQINRQNLNEIENGKTK
                     ASKEMKHILLHYLDYCNCTQPFTLTIDYLRVRFPTTDALEIIKNVLAMKSEYFIHEDY
                     GMYAYEEQYIYGDISVNASKDSSMGVLLELRGMGCRDLEYVLQVRGIDWYCFLRCCID
                     YQGVFKRIDLAVNDMGGLLDIEILRERYYANKVWKRSRTHEAVDSGKLSGTHGDTAKT
                     FYIGSKNSSIYFCLYEKEKEQKSKGIKTDIKNRFEIRLKNGKAGQTVEQLVFSRNPEQ
                     TIASFILTQIDFPDYILWDIFLDNVTTSLPFIMTPVAVNMDKIKRWLERQVMPSLLMI
                     KEIEKKTGANYLEEIDRHTRLTEKQ"
     gene            46270..46483
                     /locus_tag="KPH29_000840"
                     /pseudo
     CDS             46270..46483
                     /locus_tag="KPH29_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
     gene            46595..48208
                     /locus_tag="KPH29_000841"
     CDS             46595..48208
                     /locus_tag="KPH29_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG1466469.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFERPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIVEKIFSLAYQGYGSAKITKVLREEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     ATNTANKTPYSYYACSYYGQFGKGTCSMHYIRYDVLYQAVLKRLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASAFKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITNLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEAITNEDNERIQEIEIYYRFIGKVD"
     gene            48237..48560
                     /locus_tag="KPH29_000842"
                     /pseudo
     CDS             48237..48560
                     /locus_tag="KPH29_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
     gene            48592..49779
                     /locus_tag="KPH29_000843"
     CDS             48592..49779
                     /locus_tag="KPH29_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBG1466470.1"
                     /translation="MSKIVFFSILAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPGLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIK
                     PKERKRKLIYISLGTINNKNVSFYKNCINAFKDSDVDVIMSVGRSTDIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFITHSGMNSVNESLYYGVPMVLFPQHSEQRMVAKRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            49971..50375
                     /locus_tag="KPH29_000844"
     CDS             49971..50375
                     /locus_tag="KPH29_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HBG1466471.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNCIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYHALYVNKVMLQI
                     YDVENNNKLLRSNEELKSVKYPPIYLKYKPDK"
BASE COUNT        20236 a         5520 c         8798 g        15883 t
ORIGIN      
        1 aagaaataaa ttacaaaata tgtgataaat taagtataga ttattataga aaaaacttag
       61 tacactataa aaataataga gttgcaataa tggatttaaa tagttatctt attaaaaaat
      121 ctgataatct atcaaagaaa atatcgatta acgaaagatc ttatgaaata tttaaggatg
      181 agaaattcat aagtagtaaa gtaggaaaag aagtactaaa aaacttaaaa atagatctaa
      241 taaaggattt aaacgtatat aagacaccag agccattcat atatttatca ataaatcgta
      301 tatctccaca aaaaatatta attatagaaa ataaagacac ttacataaca ataacaaaaa
      361 tgctattaga aggaaaagaa atacttaaaa ataaaataga tactgttata tacggagaag
      421 gaaaaaaaat aatcaacagt attaaaggat taaaggaaga tataacaatt gaatatctta
      481 caaataaaga aaatgaattt ttatattggg gagatataga taaaactgga ttatcaatat
      541 acacttccct aaaagatacc aatgaaatta ataatataag attatttgaa gttgcttatg
      601 agcatatgat agataaaaca aaggaaaata ccttgaggaa tatccccaaa aatcaaaaag
      661 ataattttga atgtggatta gaaaatatta ataacctaaa cttaaaagaa agtattaaaa
      721 atattttagt acaaggaaaa tacatacctc aagaagctct aaatagattc gacttggagg
      781 aatataacta atggaaatga ttaatatttt agaaggctac aataagagag taaactgtat
      841 aggatcttat tatcttattg caactcttgc taacaataga aataaattta aagaatttga
      901 caacattcaa ttttacaatt tattaattca agttttgtgt tatatatttg atagatctct
      961 tagaagaaag aactgtttaa gagatgatat taaggatttt attgaagaaa taaatcgtat
     1021 ggattataaa ataatgcttt cagaggatga cttaaaggat ttagcaaact atataattaa
     1081 tggattaacc aatagtggta aagtatatct atttacctac tattctttag aacaagaaaa
     1141 acatatagat gaaagtatta aaattataga agataaaaat gtaaaaataa ataatcagga
     1201 aagactatcc tactccttaa caacagaggg ttatagattg ttgttatcaa caaaagaata
     1261 tgatgaacta ttccaaattc aaatatcaca aatgattgca aagttaagga tagaaaaggg
     1321 tgattatcaa ggcgcaaagg aagatattta cgatataata aataccttag agattcatca
     1381 acagaaaatt gataattata taaaaataat aaaagtaaat atactatata taaaagaaga
     1441 aaaatattca acagtaatag atacaactgt agaaacactt ttagaagaaa tggacaaata
     1501 tgaagaatta aaaaaagaag taaaactatt aatcaaagat aaagaggact acttggaaaa
     1561 ggatgaggat atagacaaag aaaagataaa aaatataaga actcaactat tacaaatgaa
     1621 agaattaata gatggaatag ttttagcaaa gtctacagcc acaaatttgt tatctagagt
     1681 tcaagatttt agagaagaat ttaaagaaat acttaaaaaa ctattaacaa ctccagtaat
     1741 taaaagattc aatttcaagg aagaaatctt agatagactt gaaaaagaca gtgatttttt
     1801 attgataata agagaattat atatatcttt atttaaagta gagttgccaa atcgatttaa
     1861 tattggagtg gcatatcaag aacagaaact attaaatact agagaaattg aaaatgaaat
     1921 agttaataaa gacgagttag aaattattga aaataaagag aaggaggaac gtataaatta
     1981 ttgtgaaaat tactattttg atttttttaa atacttaatt aaatatggac ttgaaaaagg
     2041 acaatttaca ttagaagaat ttctaaaaaa ttatctgaaa tatgacaaag ttatttactt
     2101 agaaataact aaagactcca aattgatgag agatataata atttacttcg ctaatcaaag
     2161 ggaaagtatc aatatcaaaa agataatcag aatgttagaa aatagtgcct tctcaccaaa
     2221 tacagatttt caaattgaaa gattaataga acgtttatta gtagaagatt ttgaactatt
     2281 aaatagcgtt agccatatta aaagtaataa aatatttgaa gatgaaatga aaataaatac
     2341 tgaaataaat ttagaaacta tgactgcagt taagctagaa atagcaaata taaaatttga
     2401 aataatctaa atataagggg aaatacaatg aatatatctt taaatgacta taaaatagcc
     2461 aatgaaatat tttatagatt acttaaaaaa agtgagttaa aagaaaaaga agctaaagaa
     2521 ttatttttag cttatataaa taacagtaat gtaaggtatg ctttagaggc aatttgtgaa
     2581 gtatctaaaa taaaaataat attaatttca gataccttat acttaatacc aaataatgac
     2641 aatgatattt ttgggtttga ctatagaaga gaatctttat tagggaaaga tttaaaagat
     2701 agttatttag gatatttgat aatgacaata atattttctg aatttacaaa tggggtagca
     2761 ccagcatcat atattcaagt attaaactta ctggatttag tacaagaatc cttagaacgt
     2821 tcaatagctc gtgataatct agaagaaata gaaaaatcat gtgagtttaa tataactatc
     2881 ttaaagaact attgggaaag taaaagaaag tgggacgaat ctagtaagaa aggaatagaa
     2941 acaacatcag cagatttcca aatagggttt attagaaaaa taattaattt cttaaaaaat
     3001 gaaggcttaa ttgtacacat tgaggatgaa gataagataa aaccaactag aagatttctt
     3061 gatcttatga ataattattt tttagatgaa gaaaggaaag tattaataga aaaactagtt
     3121 ttaagaaaag gggaaagtat taatgccagt aattaataaa tacaaaatta taaattttaa
     3181 atttggtatg ggacaagaaa gaatacttag caacagaacc atggaactgt atggagaaaa
     3241 cttacaagta gaagcagaaa atacagcagg caaaactatg agtattcaaa ctatcataca
     3301 aagtatttgt ccactatcgg atgtagccaa accgtttact gcaatatata ataaaaaaga
     3361 accattatac tcaatgactg aatggttgct agatgatggt aaaacgttat tattaacggg
     3421 aataggattt gagaaaaagg ctggatcatc taaagaagaa gggaaagaaa aaaaggacga
     3481 tttatacaaa tactttatgt ttattataga agaatctaaa gaaatggagg ttgatataga
     3541 taatatacct ttattaaaaa acaatgacaa aggtataagg atagtacaac ctttaacggc
     3601 ctcacaagac tatataaaaa tgctaagaga taaatatgga aaaaacaaag tacacttctt
     3661 taataattac ttaagaaaac aacatagaga aaaattagaa gaatatggaa ttaaccaaag
     3721 tgaatggaaa gaaattatta taaaacttaa tagtaataac aaagaaggtg gactatcaga
     3781 ctttgttaaa gaacataata cttcagaaaa acttataaga gataaaatat tacctttaat
     3841 agagacaagc ttaacaacag aagaattcgc tccaatattg caaattaagg aacaagtaag
     3901 aaagtgtata aataatatta tagacatgaa tgaaaaatta tcagattata aaaactatag
     3961 agatttaaac attagagttg aaagtttaca aaataaaata aataggatcc tattcataga
     4021 agaagataaa aataatgtat taggagaatt agctaatcta tatgtacata tagaaaataa
     4081 acgcaatgaa ttaaatgaag aaataaaaca gtatcaaaaa atgaaagcat caaaagaaga
     4141 ggaatttaag atagtagaat atgaagaaga tagctatgag tattttagaa aagaaaaaga
     4201 gatacaagat actcaggtag aatttgataa agccaaaaat gaattaaatg ataaaataga
     4261 agaactagaa ttagatgaac aatatttaaa tatatcaaag ttcaaaagaa aaaagaaggg
     4321 gctagaagaa ggggaaaaag ataagataga aaaagaaagt gaaaaacaaa gaaaagaggt
     4381 aaatcaagaa gaaataacgg ctattataaa tcgtctaggt agcagtattc attattgttt
     4441 aaaggaagaa agtattataa ataatgaaaa acttaaagaa aatacaaaca acaatatagc
     4501 tatacaagaa aaaaagaatc aacttaatga tgacaaaagt aagctcacaa aagtattgat
     4561 aagtaaaaat tcagaaatta aatatataga aggcaaagaa cgtgatttta ataatcaact
     4621 acatgaattt aaacttaata acactgatat taaaggcttt atttctaata attttttagg
     4681 cgaagtaata gattatacta ggtatataaa ttatttaaat ataaaagtaa aatcaataga
     4741 agaaaatctt gaatcgaaag tcatgcaaat agaagaatcg aataaactta aggatgatat
     4801 aaatgaagct ataaataatc ttaaattaga taatcatgaa tcttatatga aactgcaagt
     4861 attaaaaact agaaagaaag atatagataa ttttaaagaa aaatataaaa tcattaaaac
     4921 aagatttgaa atagacaatg aattagataa ctctactaaa ctatataaag aggtaaaaca
     4981 agagcttaac aaaatgaaac ttcaagtaca ggacttagaa aataataaaa agcaattaga
     5041 aaaagagcta gaagatttac aaaattataa agatataatt ttcgaagaag aaatattaac
     5101 agatctagaa agaaatggaa taaaacctat tgaaggcatg aaatatctat taaccttaga
     5161 tggagattta gaatacaaaa ataatctcat atctaaaaat ccattgctac catattctat
     5221 aataattaca aataaagagt atcaaaaact gaagcaaata aacttaaaaa ataaaactaa
     5281 aatctcaatg cctatatttg taagagataa catagaagat acttttataa ctacatctag
     5341 taaccttgta attagtaatg gattaaatat actatcttct ttcgataaca aagctttaga
     5401 tcctgaaaaa agagaaaagg atatgaatct aatcaaagaa caaataaata gtattcgaga
     5461 taaagtggag caatataaag cagaaattga aatattagaa gaatttaaac ttctaactaa
     5521 taaaattgat tttacagctg aagacttatc catagatgtg aaaattgaaa attataaaaa
     5581 aattatattt gaaataaaca ataaaattaa gagtaatgaa aataatgaga agaatttatc
     5641 taataaaatt aaagaacttt acgaaaataa agaaaagcta ttaaataaca agaatatatt
     5701 atttaataaa ttgaaggaca ttagaaatat acaagataca tattctataa ttttagaaaa
     5761 taaaaatgaa ataactaagt taaaaacaag tattgaaaaa atcgaagaag aaaaaaaagt
     5821 tattaaaaaa tcaatagaag atattgatgt taatatattt aatttaaatt cagaaaaaga
     5881 taggataaat aggcatatag atgatacaga aagcaagata aataaattta agatttatga
     5941 acctattaaa cttgaaaatg ctaaactaga agaattagaa ataaaataca agaaatactt
     6001 agaagaccct acaaaaaaag agattgaaac attaaattat gaaatctcag tttgcttaaa
     6061 aagaatagag agtattacta tagagttaga agaatttaga caacttatta gtagtgataa
     6121 ttttgaaaat atacctatat tattcacaat agaagaactt aataaaagaa tagttttaaa
     6181 taaaagtaag gttgatgaac tacgaaaact taaatatagt attaaaagcg acatagataa
     6241 aaatcaaggt tcattaaaca ctaagaggga ggagatactt aaaaaattta atggaaaaga
     6301 acctttatct actagcttaa tagtgaactt taattttgaa gaaagaaaaa tagatataaa
     6361 aaaagatata aaggatattg gtgaaattat agaaggtaat agaactactt gtcaaaatct
     6421 agaacaggaa ttaagagact taaagaaata taaagtagaa agaaaagcta taacctatca
     6481 acctgaagat atttttattg aaagtaaaaa actacaatca aaactgcaaa atgttgaata
     6541 taatttaatt aaatctataa atagctataa aaatacaata ttagaaataa aagaatttaa
     6601 ttataagcac atggaaaaat acaaaaactt tatagaaact ttaaatgtaa ataaagataa
     6661 ttactctcga caaaaagaac aagtagaaat agtattacta gttataagag aagaaataag
     6721 caaattagaa aaacatagcg aacaactaag aaaagaaaag actataatat caaggcaaat
     6781 aaaagattat gttatggact gtattgatga atttaaaatc ataaataaac ttggtaaaca
     6841 taaaggacaa gccttattca acatatcact acctaaatct gataaaatag aaaatagttt
     6901 gagcttagta gatatgctaa tggatgaaat tgtagaagaa gcaaacctag gtgatgttga
     6961 acaaaaaata aatacctttt atattttaaa taagactctt aatataggga gaattcctgt
     7021 atcagttata aaatatgaaa taaatagaaa tgaaactata aagtggaact ctattaataa
     7081 cgaaacaaca ggaggacaga gattttgtat ctcatttatt gtgacaatat tgttgatgga
     7141 atataaaaga tatgatagaa atgcaatagt tgatgaaagt aaatataagg gtaaggtact
     7201 acttatggat aatccttttg gagaaacgtc gcagcaagat ttccttaaag aaatatttga
     7261 acttgctaaa aaattcaacg ttcaaattat atcctatact catgtaacaa atgcttctat
     7321 aagagcaatg tttaataaga tttatttaat gacagttgaa aaaactactt ctaataaaga
     7381 atttgtggat attaatgaga ttaagcaaaa taaaactgat gaattggtgt atatgagtaa
     7441 attttatata ggtaataaag aagaattaat tcaagaggat atatttagtt taatataaat
     7501 aacaagtaaa caaataaagt gattactatc agtgaggcag gtagaaactg aattactttg
     7561 tgactcatta cgactcttgg ggtaaaattg tgtataaatg tgaagaattt cgtgattgtc
     7621 agtacaaaag cagctttatt aagggtaata atagtaagac tcctcttgaa aataggacta
     7681 aaaacttaca agtatctaag ttatttcatg aaaaaagaaa aattaaagag aattataaga
     7741 ccagagggat gtgaacttag agtgaataga agtatacaag tagaaagagc tatcgcagaa
     7801 caaatttaat tttatcatac gatagcctct tgttatttta gtgatgaaat ccatcaagca
     7861 taccataact acagaaaaag aggcttttct gatggtatat tgggttcttt tagagtgtgt
     7921 gatttgctac atgttcatta ggataataat agtcagtaaa tgatattact gatagaatag
     7981 atataataaa ctaatctagt tccatattgt aatgtttatt aaagagtata cttttatgat
     8041 tataatagct attttctttg aagcttattg tacaagagat ttgaatatat caaatttgga
     8101 tataggtata attctcttag cagttttatt tatacagtct ttatctaaat tagtgttgta
     8161 tatactttga taaaaggggg atatgtaact aagttgtaat attgatgaaa ataaaaatta
     8221 aaaaatatag agaaaaactc cttatgatac aaaatgaact tgctaaatca gttggggtaa
     8281 gacgagagac tattgtacac ttagaaaatg gattaatgta ccataaaggt atccaagata
     8341 aatagttaaa aaactagata agaataagga gcctttacag aacaaattta gtgtgctcgt
     8401 gcgatagccc tttttttata aaaattaatc taataaccta tataattaaa tttataaaac
     8461 atatatgaaa gggtaaaact atatggctaa aattgataat atgatgtcca tattatggat
     8521 gttaaactca gacaaaaaag ttaccgcaaa acaaatatct gaaaaactag aaataaatat
     8581 aagaactgtc tacagacaca ttgatgcatt atctgctagt ggagtaccta taatttcaga
     8641 tacaggtcat aatggtggat atacactttt gaataatttc atcaaagcac ctcttttttt
     8701 cgatacagaa gaacaaactg cgttacttca tgcagctata tttgctaaag aagagggaag
     8761 tttcttagat gacacactag acaaagcaac ctcaaagtta aaaatgtatt caaatcaaaa
     8821 acaagaagaa actttaacaa aacatttaat aggttttgaa gtaataaaac caactggaaa
     8881 aatatccata gaatctgtac ttaaaaagtt aaaaaatagt atagcaaatg aattatctgt
     8941 agaaatcaac tatcgtacag ctcatgaaga aaaatcaaaa tgtaggatta taaatccata
     9001 tggaattgta cgttggaata ataattggta catagtagca ttttgtcaaa tgagaaatga
     9061 gattcgtagt tttagggtag atagaattag tagtgtcata gaaacatcaa atacttttaa
     9121 taggccaaaa gaattttcag caagtgaatt ttttataaga gggattatgg atgaaacaaa
     9181 agatgaggat aatcttatat cattagttat agaaggaaag atgaatgcat tggataactt
     9241 atgtcagcat tggcttctag gatattattt aaaagaaagg acttcaaata aagcaatatt
     9301 taaacttcaa gaagagataa tatttacata tgtaccgaat atacttatac cttatagaaa
     9361 atctatacaa ataattgaac cattaagcct tagggataaa atggttgaag atttactcga
     9421 attaattaat cattatcaaa tttaataact taactgacag agtatgtcag ttaagttatt
     9481 ttacaattag gtatattaat taattataga aggagcaaaa aaatgaaaaa gagaaaagta
     9541 tatatgtatg tattcgatac tatgtcagat tgggaagttg gatatctaac tgctgaatta
     9601 aatacaggaa gatattttaa aaaaggatta aaaccactaa aagtaattac agtaggagtt
     9661 gataaaaatc ctattataac aatgggtgga ttaaaagttt tacctgagat tacaattgac
     9721 gagcttaata ttgaaagtaa tgacttatta attttgccag ggggaaatac ttggacatct
     9781 ataggtcatg agaaaatatt agaaaaatct aaagaggctt tggaacaaag gtctatagtt
     9841 gcagctattt gtggtgctac tttagggtta gctaaaacag gtttactaga ttttagaaat
     9901 catacaagta atgatttaga atttttaaaa atggtgatac taagttattc tggagaaaaa
     9961 tattataaaa tggaatctgc ggttaatgat gaaaatttaa ttactgcttc tggaattgca
    10021 ccattagaat ttacagtaca agttttaaaa gttttagatg tttttaaaga agaagcatta
    10081 agttcttggt tgaaccttta taagacgcat gactctaaat attttttcga actaatgaat
    10141 tgtaataaat aaatttaaga tttaatttta aattcaaatt ttaaattact ttttctatgg
    10201 caatagataa gtagtttaga attttatcat atatataatt tgtatttata ccaacacaaa
    10261 tagaaatgat agttccaata ataaaattta taattagttc catatggagt acatttcttt
    10321 cctactaata taactaagaa tatatgaaac caccatagat tgtagttttt tctgttgtat
    10381 tatatcatgg gatagtaaag tgacgtatat ttttgatgaa aacctaggat tgtatatgaa
    10441 atgaattaat cctgtgtatt aatcttgaga ttcgtgtcta atgatttgta aaaataaata
    10501 atgaaatact tagaaatact tgtaaatgct agtcaatatt ggctagtatt ttttatttta
    10561 gtaatgaaat ccccaactgt acttcatcca caaaaaaaga gatatttttg agggtgtatt
    10621 ggagtgaact taggctgtat aattatacat ttcaatatct tatattaatt aggataatag
    10681 ttggtaaatg atatgattga aacaatagat ataataaact aatctagtac tgtactgctt
    10741 actaaagaat atatttttat ggtataagaa ttttttagag cgttgattct ataggagtga
    10801 ataaaaaagt aattttataa tattatttgt aaccattaga tgtctattat gaaatctttt
    10861 atggactata tttgtaaaaa tattattaag tatttacaat attttcttgc aatttatttg
    10921 gaaaggttat atatttataa catgaagtgt tataaatata taacctttaa ggtggatttt
    10981 atatgtctaa atcagaaact ggtgtaaaat caaaactcat attggaatta cttgtaataa
    11041 cttatctttt tatagctgta ttagctacta agtatatttt taaaggtcca agtactgcat
    11101 ctcttccagg actgagctgg ttaatagtct ttggatgcta ttcaatttat aataaaaaat
    11161 ataatgattt agttgatgaa tcatctaaat ttatactatc aaaggttaat cagattgcaa
    11221 taacaataat gatgtattct atagttataa tagctatttt ctttgcaaca ccttatataa
    11281 gtaatataaa tatatcaaac ttggatactg gtataattct tattgcagtt ttatttatac
    11341 aatcgttatc taaattaatt ttgtatatat actttgataa aagggggata tgtaactaag
    11401 tggcaatatc gatgaaaaca aaaattaaag aatatagaga aaaactcctt atgacacaaa
    11461 acgaacttgc taaatcagtt ggggtaagac gtgagactat tgtacactta gaaaatggaa
    11521 aatataatcc ttctttaaag ttagcgatgg atattgctaa agtattcggg accacagtag
    11581 aaaatttgtt tgaatttatt gaagaagatt agtagatagg atataaatat ttataagatt
    11641 gttaataaaa tatagttatt taattattaa ttttgttaaa aatatttata aataatataa
    11701 tgtatgagta aagttaggtt gtaattattt taagataaga agactactgt aaatgataaa
    11761 aattagtgaa tatacagcca attaataaat aatagataaa actttaaagc ttagatgtga
    11821 ggatgagata tggtcttaca gtagttcaca cttttaatta agacttaaaa tggaaaccat
    11881 acacatacat acactattta ctgaaagtgg actagataaa aaaataataa gtgatttaag
    11941 aaagtatctc atatactata taagtattat tttaggtgac ctatttatag gtcatttttt
    12001 atgtatataa atgataataa tactaatatt taaaataggt tttaaagaaa taaaatatcc
    12061 agattgaaaa atggattaat gttaataaaa aaattatatg tataaaaata ggtataatta
    12121 tagaaaaaaa caataataaa ataagctgtt atagaaaaat acaattatat agattgttgt
    12181 tgaatcattg actgcaagaa taataacatt taaaatgaac atggagaata tgggggagga
    12241 atcaatatga agagaaaaat agcagttgtt ttattaactg tagcaatgtt attatcactt
    12301 gtagcttgta gcaatgataa cacatcaaaa agtgataaat tagaggatac attagtagta
    12361 tatactacac atggagagga tatgctagga gcaatttctg aagaatttac aaaggaaaca
    12421 ggagttaatg ttgaatttat aaacttaaaa ggtgaactag cagaccgtgt tagaagtgaa
    12481 aaagataatc cgcaagcaga tataatgttt ggtggagata catctacata catgcaatta
    12541 aaagaagaag gattatatga aaagactgaa ccaagctgga ataatgaatt agacaagtct
    12601 tacaaagatt cagatggata ttggtatgga actataaaaa caccagtaat gatgttttat
    12661 aacacaaaac tattatcagc tgataaagcg ccaaaagatt ggagtgattt aacaaaagaa
    12721 gaatacaaag acttaatagt tactagagat tcattatcat catctatgcg ttctactatt
    12781 agtaacttaa tcaattacta ctcaaatact aagagtgaag aagaagcttg gaagtatctt
    12841 gaggatttaa ataagaatat aaagaattac tacaatagtg gaagtgtcat gtattcagca
    12901 ataggtaaag gtgaagctac aatatctgtt gcagtattaa gtgatataat agagaataaa
    12961 gaaaaaaata atatgccatt agaagttgta gatgcagaga gcggttctgt agtaatcaca
    13021 gactgtgtag cagctctaaa aaatgcacca catccaaatg cagcagctaa atttgtagat
    13081 tttgttggaa gtgctaaggt tcaagctatg gttgcaaatg attttaatcg tattccaaca
    13141 ttagattctg cacttaaaga ttcaccaaag tggatgtcaa aagaatataa agctatggat
    13201 gtagattggt ctgtaattgg aaaaaatcag tctaactgga ttgaaaagtg ggaaacagat
    13261 ataatagata acaataaaac tataaaatag cctaattaaa taatttaata ttatatacaa
    13321 aaatagtgag gattttcctc gctattttac gtataaggaa actatagtat ctcataattt
    13381 tgtataattc atacaacaaa atgatactaa tacatactta ttaaataaaa tagtttttga
    13441 gtaactgatg aagtaattga ctatataaag tgatttgtag acatagtctc tcaaataaag
    13501 tgaatttttt attatgcaaa acttttaatt atagtaaaag tacaatttat atgggcaagg
    13561 agataaaaat ggaactaaaa attagtgatt ataaattatt atgtaataaa ggtagtggcg
    13621 cttttaacga agatatcgta ggtttatcac cttatggggc atgggtactt gatggagcta
    13681 caggtttaaa taataaaaat ttagtctcta cagaaagtga tgccaaatgg tatgtaagtt
    13741 ggtggaatag ttatcttcat gagaatatgg aaagagaaga tagcttaaaa gtaattatta
    13801 aagaaggcat tgaggagata acaaaagagt actacaaatt aacagatgat aaagaagtag
    13861 aagcaataga tattccttct tgttcatgtt ctatattaaa gttttataaa gataagatag
    13921 agtatttact tttaggagat tgtactttat tagttaacaa taatgaatct gtgaaaattt
    13981 taaaagatga gactttatgt gaacttgata aaaaagtatt tgattttatg aaagaagcca
    14041 aagatgctac aaaccttact ttaaaggaga gaaaagccga ggtaatgcga ttataataga
    14101 gaatagactt aagaaaaata ctgtgggggg atattggact ttggaatttt ctaaagaggc
    14161 cgttgacaat tgtgtaagtg gatttattga tatagaatat aaaatgaaaa ttatgatggc
    14221 tagtgatggt ttttcatgtg gatatgatag atataagatt tttgataaaa atgaaatgat
    14281 gaaaatagca gagattaaag gtatagatta tatatataga aaggttaggg aactagaaaa
    14341 ggatgatgaa atggcaatag agtttcctag atttaaaatc tatgatgatt catcatgtgt
    14401 atatttagag atttataaat attagataat ttatggagac gatatgaaga atatagaatt
    14461 aaaagaaatt aatcattatt atggaaaaga aaaagtatta catgatatta atatatctat
    14521 taaggaagga gagtttttta ctttactagg tccttctgga tgtggaaaaa cgactatttt
    14581 aagaataatt ggtggattta taaaaccttc ttctggaagt atttatgttg gtgataaaaa
    14641 cattaccaat ttagaaccag aaaatagaaa tatgggaact gtttttcaaa actatgcact
    14701 gtttccaaat atgacagttg aagaaaatgt atcttatgga ttgaaaataa aaaagctgtc
    14761 taaaaaaatt ataaaggaaa aatgtgataa ttatttagag ctgtcaggaa tgaaagattt
    14821 taggaaaaaa aagattgatg aattatctgg tggacagcaa caaagggtgg cagtagcaag
    14881 gtcattagcg actgagccta ctatgctttt attagatgag ccaatgtcta atctagatat
    14941 agctttaaga atcaagatga gagaagaaat aagagagata cagcaaaaga caggtattac
    15001 tactttattt attacccatg atcaacaaga agccttagct atttctgata gaatcgctgt
    15061 aatggataag ggtaaagttc ttcaaattgg tgtgccaata gaagtttaca aaagcccatt
    15121 gaatgatttt gtagcaaact ttgtaggtac ttcaaactgt attggaaaag aagattatgt
    15181 taattttaat attgaaaaag agacaaagcc ttatatttat aaaagacctg aagaaatgat
    15241 attattgcaa aatacaaatc aaagtggttt tgtaaaagta aaagtagaat ctaagaattt
    15301 tctaggagca attttagaat atacagtatc aaatgatggg aaaaaatatc aagtaactga
    15361 gcttaataga ctgaataata gcaataaatt aaacgtaggg gatatggcat atttaggtgt
    15421 gctacaaggt gactaatatg attaaaaaaa taaaccgtag taaatttaca tctaaaaatg
    15481 ttatttttat tgttttatac agtatattat tttggatttt gattggatat attatcatac
    15541 caataggcaa tacttttgta caagcattcc aaggtaaagg tggatattct tttgaagttt
    15601 ttaaagaata tttttcaaat actaataatt taagggtagt aagcaatact ataattttgg
    15661 gattgggttc tgtgttcgta tgtggaatta tgggaatatc acttgcctta tatatgacat
    15721 ttgtttgtga taaacataag aaaatcatac atattctttt actaagtcca atgatgattc
    15781 ctggagtaat tatggtaatt gcatgtattc agatttatgg agaaagtgga atagttacaa
    15841 aatctataga aattttactc ccctttatca aaaaacctta tgattttagt ggattaaagg
    15901 gaattttatt tgtggttgct tatactcagt atgtatattt ttatttaaat gtatatgttg
    15961 ccttaaaata tgtggattac tcaacagttg aagctgcaaa aggaatggga gcatcaaagc
    16021 taagagtatt ttttgatgta atatggcctg ttattacacc tgctgttata acttctacta
    16081 ttataacttt tgcatcaggg attagttctt tctcagctcc aaatcttata ggtggtggtt
    16141 ttaaagtttt aagcactcaa attgttaggt ctaaggctaa taaccatatg gatatagctt
    16201 cagttcaagt aattacttta ttactaatgg ggattttatt tatgatttta ttacagtatt
    16261 atggcaaaaa atatggagta gtgccatcct taaaatctca aagttataaa gttcataata
    16321 gaaaaaaaga cttatttaca tttgtatgta aggttattat tgaaatacaa attattatga
    16381 ttatgcttcc tatagttact attgtgtatt tatcctttgt aagcacacat tcaattatga
    16441 caaccatatt tcctagagac tttacactgg aaaactatca gatgattttc caaaaatcac
    16501 gtgtattaca gcctatatta aatagtttca aaatgtcttt aatggcagta gcagtaggat
    16561 tgataattac tgtaccagta tcatatttag taattaaaaa ggatactgca tataatcgac
    16621 tagcaaaatt tattataatg ttaccttgga gtatgcctgc aagcgccatt gcagttaacc
    16681 taattaatac atttaatcaa aaaagtatat ttgcttttaa tgaatcctta ataggaggtt
    16741 tttatattgt acctatagca tatacaattt atgctctacc attattattt tcatccaatg
    16801 aggttgctat gaagtcaata aatttaggac tagaagaatc atcaaggagt ttaggtgctg
    16861 gaaggataaa aaccttttat aatgtaatag ttccaaatat gatgccagga ataatttcag
    16921 gagctatatt agtattcatt cgtacaattg gggaatatac aatgtcagcc ttgctttatg
    16981 gtgtttataa tagacctatt tcaatttcta ttgtaactaa tatgcaagaa tttaagatag
    17041 gagtatctat ggcttatggt gtattagtaa ttggtatatg ctatatagca ttaggaataa
    17101 tttttaaatt agatagaaaa agatttacat aagataacaa aggctaattt ataaattggt
    17161 gcttagaaaa ccaaatcact ttattagcta aagatgaatt tgaagggtga cactttggtg
    17221 ttttatgtaa agtttatgga agtaaaacat taaataatcg tgagtatgat tcctataaat
    17281 cgtatatact attgaaatcc aagaaaaggt attggctaaa tagctaatat ctttttaatt
    17341 ttttgactcc catatagcat taactattac atgagtatct ttaaatttat agttaaagta
    17401 tcttaattgt ttattataaa aatataggat ggctaaagca cattaaatta aaacacttaa
    17461 aaagagaaga tgtacatgag aaatataatt aactatgata cagacttcta aaaattattt
    17521 agtgaaaagg aacttagagt gtataataaa agttcctttt caataaggaa aacgattgtt
    17581 attttctgag cttcattacc ctgatattat ttaaatataa acaacaacaa aattaacaag
    17641 aagtaaattc aacctaaaat ttaaaatatc gggaggtaca atatgttaat caatatgaag
    17701 gaaatgctaa aagtggcaca agaaaatcaa tttgctgtac cagcatttaa tataggaagt
    17761 gggcaaatac ttaaagcagt agtacagggt gctaacgaaa aaaatgcacc agtaatactt
    17821 gcaatacacc caaatgagct aagttttcta ggcgatagct ttgtagttag ttgcatagaa
    17881 gaagcaaata agagtaaagt acctatggta atacacttag atcatgggga aaataaagaa
    17941 caaatattaa gggcaataag atgtggattt acatcagtaa tgattgatgg ctcacattta
    18001 ccatatgaag agaatgtagc tatatctagg gaaatagtag aaatagcaaa aggattaaat
    18061 gtgtccgtag aaggagaact tggaacaata ggaacaacag gaacatcaag tgaaggtggt
    18121 actgatgaaa taatatatac agattcaaaa ttagctaagg attttgttga aaaaacagga
    18181 gtagacacac tagctattgc aattggtaca gcacatggta tatatccaaa aggttttaaa
    18241 ccagagctta aactagattt attaaaagaa ataagagaag tagtagatat acctttagtt
    18301 cttcatggtg gttcatcaaa tccagatgaa gaaatagcac aagcagttaa actaggagtt
    18361 tgtaaaataa atatttcgag tgatgttaag tcggcatatt ataagaagtg cagagaacta
    18421 ttagaacaaa acccaagctt atatgagcca gatactatat atccaccatg tataaaagct
    18481 gcaagagaag ttatagagtt taaaatgaat ctattcaatg ctatagataa gttaaagtgc
    18541 ttttataaaa ataaaaatta attatgaggt aacaaattaa aaggagggga gaatttatga
    18601 taggtaaaat actacgaagc gattgtattg tactagataa acaattacaa acaaaaaaag
    18661 atgtaattaa ttatatggca aatcatttgt accatggaaa ttatataaat aataaagaaa
    18721 agtttatagc tgatattaat aaacgtgaaa aagaatcatc aactaatatg ggtaatttaa
    18781 tagctatacc tcatgctaag tcaaatgtag taaatcaacc tgtaatgatg tatttaagaa
    18841 cagatagaga gattacagat aatgatggtc aaaaacttag gtcatttttt atgatagcag
    18901 ttccagaatc aggttcagat gaacatctta agattatatc taaactagct acatatctaa
    18961 tggaagaaaa ttttcttaag catctactaa gtgcagaaac tccatacgaa atattaagta
    19021 tatttaataa taaagctgca gaatcaggag attttagtaa taatcaaaat aaaggtctta
    19081 tagttgggat aacaggatgt ccaacaggta tagctcatac ttttatggca aaaaaagcat
    19141 tagaggaagc agggcaagaa cttggatatg aagttaaaat agaaactcat ggagctatag
    19201 gtgtagaaaa taagttgact aaagaagata taaaaagagc tgaatacgta ataatagctt
    19261 gtgataaaga agttcaactg agtagattta taggaaagaa agtgctacaa aagaaagtag
    19321 tagatgctaa gaatgtttca ttagctaaaa atttaattat aaacgctgaa aatggaatgg
    19381 ctaaagaaat taaaccaggt ggaaagactt tagaagattc aaaagaaatg cttcaagggt
    19441 cagagacaaa gggtgtttat aagcacttaa tgagtggtgt atctgaaatg attccatttg
    19501 tagtgatagg tggaattggt atagccatag catttatgtt tggtatattt gcatcagacc
    19561 caactcatga aacatacaac cctatagctg gattctttgg acaacttgga ggcgattcag
    19621 catttaagtt atttataccg atacttgctg gttttatagc taaatcaata gcaggaagac
    19681 aaggatttcc tcctgctatg atagctggtt ttatggcagt tattggaggt tcagggttta
    19741 taggtggtat ggtagctggt tttgcaacag gatatatagc tttaggagta atgaagataa
    19801 cagaaaattt accagatgta ttaaaaggag taaatgctgt attgatatgt ccacttttga
    19861 ttgcatttac gagtggagct ataatgttct ttgtagttaa tacgccagca tcatggttaa
    19921 atgaagcttt acaatcttgg ttacaaagta tgtctggaac taataaagta ttattaggtg
    19981 caatattagc agggatgatg gcatcagata tgggaggacc tattaataaa acagcttcag
    20041 cttttggatt ggctatgttt tcaagtaata tatttgagcc atcagcagca ttaatggtag
    20101 gaggaatggt acctccatta gcgatatcat tagctatgtt aatattcaaa aataagttta
    20161 ctaaagaaga aagaaagtca tctctaccaa ctgctataat gggggcatct ttcataacag
    20221 aaggcgcaat accgtttgca gctgcagacc cattaagaat gattccagca aatataattg
    20281 gttcttgtat aggtggggca atctgtatga gtttagatat aactttaatg gcaccacatg
    20341 ggggaatatt tgtaatacca tttgcatgta gtgcaccagt tgcatatata gcttgtatat
    20401 tgattggtac agtaataaca gcattgatta taggggtagc aaagcctact ataagaagtg
    20461 aaaacagtga agttgaatta cttggtgctt aaattataaa taatagtaaa aataagtaat
    20521 ggaataacaa caagtatata gaataataat aaggatattt agaatcagtt aactacttct
    20581 aaatatcctt aatttttatt ttgaaattat attatctatt atatcaattt agtaaggtgt
    20641 atcataaatt cttttatgat acagaagtat cttagtaatt ttacatagtt agtaaatatg
    20701 ttaaaatagt acttaatata aacatagaaa tatataaaca taggtaggga gggctaagta
    20761 tgcttgtgtc gaggcaaatt gaatttctta aattgatact aaacgaaaat aaatatagac
    20821 ctataaaata ctttaaagat aaattaaatg tatctgataa aactttacaa aaagatttga
    20881 agatgattga gaaatattta gaaactttta atataaaaat tgatataaaa agaggctatg
    20941 gaatattaat agaagattta gcaaagaaga acatagactt aattaatagt ttaaatatac
    21001 aatcgaagga aaataaatct ttatcagtag agcagagaag aatggagatt ttaaagtatt
    21061 tattactaaa ttcaaataat ataacttcaa taaatcagtt agcagataaa tattatgtaa
    21121 gtaaaacttc tatagtaaat gattttaaat atatagaggc atggataaaa gaatacaatt
    21181 taaaacttaa taaaacttta gaaggaacta agattatagg taaagaagtg gatataagga
    21241 aatcgattgc caaaatgatg gatgatttat tagatgaaaa ttatgaacaa catgatatat
    21301 ctgagttaac aagattagat tctgctacat ttagtgcatt gataaattta tttgatattg
    21361 attctattat atttgtagaa actataatta cagaattaga atacaattta ggatatacta
    21421 taaatcagcc ctactatata aatttaatta cccatatact tatatgttta aagagaatcg
    21481 aagaaggtaa tcaaatagaa tctcaggaag aaagagaaat ttctgtagat aatttggacg
    21541 agttagtata taaaaatgtc actacactta tagataaaat agaaaataga tacagagtaa
    21601 aaataacgaa agatgaaatt atgtacatat atattttttt ggtatcatct ggatttagta
    21661 gcgaaaataa tagtagttat aatgatgaat cagatatatt aagtgaaagt gaaaaagttt
    21721 catctataat gatagagaat atgtcacaat ttttaaatat aaatctcaaa aatgatgact
    21781 tactacaaaa aagtctagca tctcatgtaa ggcctatgtt aaacagatta agatatgata
    21841 tccaaataaa aaacccgttg ttaggagaga tacaagagcg attttcagat gtgctgggat
    21901 tatgtatgat gactataaat attatgacag agtatgataa tctgaagaat attagtatag
    21961 atgaagtttc gtatcttgcc acatattttc aagcggcaat agaaagaaat atgagtagta
    22021 aaagagtaat agtagtttgt catacaggat atggaacatc tcagctcctt gcagccagat
    22081 taaaaagaga atttcctgaa tggactatag ttgatatagt tcctatgcat cagttgagta
    22141 agcgaaattt agatgatgta gactttgtaa tatctactgt aaacttagat ttgaaagata
    22201 aattacatat agttgtatca gtactattaa tggaaagtga tattaataat ataaaaaatg
    22261 ctttactttc taaacctaaa aaaagtatga acttagatac aaacttatta ggaatgtatt
    22321 tagaagataa tatatatttt aattttgatg tagctcagat ggaaagttta ataggtgtga
    22381 atttagggag taaatatgaa tctatttcat taggaaaaga tttgaatata tatatacata
    22441 agcaaagtaa attaaataaa gggataatga atatcaataa tgttactaga acgattgata
    22501 tttatttaga aattagtaat caaagttttt taaagaatat tttaatggaa atatcaaatt
    22561 tatataggca aaagaactat ataaactata taatagattg caagagcaaa gaggatatta
    22621 aaatattatt taagaataat ctattggaga aaacaaatgg aaattagaga aatattaaca
    22681 gaagaaagca ttattttaga tttgaaatca actacaaaag aaggcataat acaggaatta
    22741 gtagataaat tatacgatac aggaagtata actttaaaaa gtaagtttat aaaggcatta
    22801 aaacatagag aaaaaatagg ctctactagt ataggttatc aaatggccat gccacatgga
    22861 aaaacaaaat atgtaaagaa ggcatccata gtaataggta aatctaaaaa aggtgtagat
    22921 tggccaagta tagatggctt acctgtaaag gtaataatta tgtttgctat gccacaatct
    22981 ggaggcaatg aagatatgat attaatatct aaaatagctt acattttagg tggtaaaaat
    23041 ataagtgaaa gacttttaaa tgtacataca aaaaaagaaa tattagatat attttatgaa
    23101 gtgtaggtat tgtataaatt ataaatttga ggtttttaaa ataaaatgtg tattctttta
    23161 aatagtatag attaattttt agacactaga gaggagagga tatctatggc aatatattta
    23221 gaaatgccta aattaataca tgaatttcct tttcgtagtc tatcaaatga aggagaagta
    23281 ttaacaacac cacactggca taaggaaata gaaatattac ttataacaga aggtgtagta
    23341 aatctattta taaatgacaa gcctatgcaa ttaaaaaaag gcgagatagt gataataaaa
    23401 ggaggagata ttcattatat actgccatca ccgggaagtg aaagattagt atttcaattt
    23461 gatatatctt tttttaatga tttaatttta ttaaatgagg aaaaagaaag cttaattaat
    23521 ttattcagta gtataaaaaa atgtagtaga gattggtcag caaaaataag aaagtcagtt
    23581 ttcgatatat tgatagatat ctacaatgaa gacctataca aaatagaagg atatagttat
    23641 gcaataaaag gtaaaatgta taatttaatt cctatattat atagacagat tcctagagaa
    23701 attgaagaaa atacagtaga atatgaaata aactctaaag agatattaga gagattgaac
    23761 aatatattca aatatgtaga aaaaaattat aaacaaccta taaaattaga agatgtgtct
    23821 tatgaagtag gatttagtgt atattatttt actaaatttt tcaaaaaaaa tacaggaaaa
    23881 acatttataa cttttttaaa tgaatataga ttagaaaaag caaagtggat attacttaat
    23941 aacaatatat ccattgtaga tttggttgaa gaagttggtt ttggaagtac aaaaactttt
    24001 tacagagtat ttaaagaaaa aattggagtc tctccattag aatttaaaaa aagatataat
    24061 agcttggaga aaaaaggggg tgcctcaaaa tgaacttagt tcatgaggca ctttctttta
    24121 tatgaaatca aatatatttt cagtatttca acaatgaaaa ttccattcat ttataaagtc
    24181 taaattttag ttctcaatac aatctctatt tcgaaataaa tccttttata tatataaata
    24241 tacagcaata tatgagaata agataacaat aatgaggata taaaaatgta tttgctatat
    24301 atataattat atacatagat atctaatatt gatatttaat atgaaagtat tgaaaataga
    24361 caatattaat tatagtgagt aaaaaatata attggatttt taaattgaat tatttaaaag
    24421 gtatagtatg aaaaaaatct tataactaaa tatgagatag aaattataaa actttatact
    24481 gtgaaagagg tagatgcatg aaattaggga aagatttttt atggggaggg gcaatttcag
    24541 ccaatcaaag tgaaggattt tatttagatg gaggaaaagg tttatctcag tttgataaac
    24601 tcccattaaa tgataagaga ttaaaagatg tatgtttaga tgaggataat attttaaatg
    24661 aaaataatca atactatcca agtcatatag ggattaaatt ttgtgaaaat tacaaagaag
    24721 atatagcaat gttagcagaa ataggtttta aatgttttag attttctata tcttggtcaa
    24781 gaatattccc aacaggagaa gaaagtgaac caaatgaaaa gggattacaa ctttatgata
    24841 atataataaa agaattaaaa aaatataata tagagccatt aataacgata agccactttg
    24901 atatacctta ttctctagtt gaaaaatatt ctgggtgggc tgataggaga gtggttaatt
    24961 tatatgtaaa atatgctgaa attttgatga aaagatataa agatgatgtc aaatactgga
    25021 ttccttttaa cgaaatgaat atgataattc atattccata tataggtgct gggttaactt
    25081 ttatagctga tgaaaatcaa cttcagaaaa aatatcaagc agcacaccat caattattag
    25141 ctaatgcaaa aactatagaa ataggaaggt atataaataa agaatttaag tttgggtgta
    25201 tgttagctgc tggaaaaaca tatccataca catgtaaacc agaagatgta tttgcaactt
    25261 tagagcatga aaggcataat ttgttttttt ctgatgttca agttaggggt gtgtatccaa
    25321 gctttatgaa ttattattta aaatcaaatc aaataaaatt aataataaaa gaggaagatt
    25381 tatcaacact taaggataat actgtagatt ttgtatcttt tagctattat tctagtgctt
    25441 gtagttcagc tagagctgaa ggtttagaaa aatcaaaggc aaacggacca gaaaccataa
    25501 aaaatccata tcttccaaaa tcaaatagtg tatggcaaaa cgaccctgta gggttaagaa
    25561 taactatgaa tcagttatat gaaaggtatc agttaccttt atttatagta gaaaatggac
    25621 tgggtactca agatattgta aatgaagatg gaaaaataat tgatgattat cgtatagatt
    25681 acatgaaaga acatattaaa aatatgattc ttggagtaaa tgaagatgga atagagctta
    25741 taggatatct tatgtgggga tgtatagatg taaccagtgt aagtgaagga aagatgacaa
    25801 aacgatatgg cataattcat gttgattctg atgactatgg aagaggctca tttaaaagaa
    25861 ctaaaaaaca aagtgctttt tggtataaaa aagtaataga aacagatgga gaaattttat
    25921 ttaatgatat ggaaactcat gcataattca ttttaagaaa ggagtgttat tatgagttta
    25981 atatttacta ctaaggacca acttatagag caagggttta gtaaggatac aataaaatcc
    26041 aatgaaacgt tgtttacact agctaatgga catttaggag ttagagggaa tattgaagag
    26101 tccgatttta ataatgagta tgtagataat atgggtacat atgtaaatgg attttatgaa
    26161 gaatctccaa ttacatatgg agaaagcgct tatggctatg caaaacaaaa tcagactatc
    26221 tgtaagttac ctaatgctca tatagtaaat ttctctattg aaggagattg ctttgattta
    26281 tcaaaaggta ttacaagtga acatactagg atactagatt taaatgaagg aattgttaaa
    26341 cgttcattta tatgggaaaa ctctcgtggt agaaaagtta aagtatctat agaaagatta
    26401 gtatcacaag atatagaaga attaatgatg atttcatata caataacacc attgaatttt
    26461 aatggaaaaa taaaatttaa aaataaaatg gaaaatagta tggagaagtt taattgtgaa
    26521 aaagtagatg acccaagagt ggcatctata aataagaagc aatttaaaat taatgtaaaa
    26581 aaatataaca ataagtattt tatgaattta gttacagaaa gaagtagatt agggttatgg
    26641 tgtgggtttg attcatatat tacttgtgga aatattacaa atgagaccta taatgtttca
    26701 gatgaatgta ttgagcgaga aatagaagtc tatgccaaaa aagatgaaag gttaaattta
    26761 gaaattgttg taggctatag tagagtatat tctgatttaa atctaaatga agaacgttta
    26821 aaatcattag aatgtctgga gtcgttaaaa tgtttagaat cagaagcaca tttaaaatca
    26881 ttagaattgg ttttaagaga agcatgtaat attggatttg ataaagtcaa aaatattcag
    26941 agaaagaaaa tgaatgagtt ttggacaacc tctgatgtag agattgaagg cgataaaact
    27001 cttcaactgg gtataaaaac caatttattt catatttatc aatcagcagg aagagatgga
    27061 aagactaata tatcagcaaa aggtcttaca ggtgatggct atgaggggca ttatttctgg
    27121 gatacagaaa tgtatatatt accatttttt atatatactc aaccatccat tgctagagct
    27181 ttattagatt atagatactc aatcttagat gcatcaagag aaagagctag ggaacttgga
    27241 ataaataaag gttgcttata ttcatggaga actataaatg gtaatgaatg cagtgcatat
    27301 tatccagcag gaacagcaca gtttcatata aatgcagata tagcttatgg tattagaacg
    27361 tattttgaag caactaatga tgaagaattt atgatgcaaa atggcttaga aattcttata
    27421 gaaacagcaa gattttgggt tgaatttgga gattatatag aattaaaaaa taataaattc
    27481 tgtataaatg gagtaacagg accagatgaa tatacagcaa tagtaaacaa taattactac
    27541 actaatttaa tggctaaata taatttaata tatgcagtac agatggtaga taaatttaag
    27601 cataatttat tttcaaatga agtatttaga aaagttaaat tacaagaata tgaagtaaaa
    27661 gactggataa atgccgctca aaatatttat ttaccatatg atgaagagaa gaaaataagc
    27721 aaacaagacg attcattttt agaaaaatca gtatgggatt ttaaaaatac accaaaagaa
    27781 aaatatccat tacttctcaa ttatcatcca ttaacaatat atagatatca agtaaataaa
    27841 caagcagata ctgtactttc agcgttgctt ttccctgatg agtttagctt agaacaaaag
    27901 aagagagatt ttgaatacta tgaaaaaata actactcatg attcatcttt gtcaaggtca
    27961 atatttggaa tgatggcaag tgaaattgga aatcatgaaa aagcttataa ctattttatg
    28021 gacacagcat taatggatat taataaccta caaaaaaata caagtgatgg aatacatgct
    28081 gctaatatgg gtggaacatg gatgagttta gtatatggat ttgcaggaat gaaaataaaa
    28141 aaggataaat taagttttga accaaggata cctaaacact ggaaaaatat taaatttaat
    28201 atattattta gagataattt agtttctgta aatatagaag aatataaatc ttattataag
    28261 ttaatcaaag gtaaaaacat aagcatatta catgataata aagaaataaa attatcaaag
    28321 aacataatag aagttaataa ttgttttaaa gggagtaatt aatatgaaag cagtcttatt
    28381 tgatcttgat ggagttataa cagatactgc taagtatcat tttgaagctt ggcatgagat
    28441 agcaaaatcg ctagatatag aaatagatga aacttttaat gaaaaattaa aaggcataag
    28501 cagggaagaa tcattagata aaatattgtt atctgctaat aaagtagata gttatactaa
    28561 agaggaaaag ttaaaggtgt gtgaagaaaa aaataacatt tatataaagt taataaactc
    28621 attaacatat aaagatatat taccagggat aaatgagctt ataaaagaac ttaaaaaaaa
    28681 taatgtgaaa ttgggaatag catctgctag taagaatgca cctaggatat tagaatcttt
    28741 gggaataaaa gatgaatttg attgtatagt aaatccatcc ttattagaaa ggggaaaacc
    28801 atatcctgat atttttattg aagggagcaa gatgctaaat ttaaaacaat atgattgtgt
    28861 aggaatagaa gattcttatt caggtattaa agctataaat gctgccaata tgacatcaat
    28921 tggtgtgggg aaaaaagaag tactaggaaa atgtgaccat attgtagaag atactagctt
    28981 attaaattac aatacactat tacaagcttg gagtaaaaag attaatttgg attaaaatta
    29041 tattaaaaat tattttaaaa gagaggataa ggtatgaaga ataaatatga agaagtagct
    29101 caattgataa ttgatggact tggaggaaag gataatataa ttgacttaac tcattgtatg
    29161 acacgattaa ggttcatctt aaaagatgaa aagaaagtca atgcagataa attaaaatct
    29221 atagataagg tagttggagt aaactcaact tcaactcaat atcaggtcat aataggtaat
    29281 gaggtaggtg ttgtatttaa tgcaataata aataagggga taaataccac aggagatatt
    29341 aaaagcgata taaaaaatga aaataaaaaa aatagtattt taaataatat aatagataca
    29401 ataactggtt gtatgacacc tatgatacca gcccttacag catctgggat gataaaagtt
    29461 atattatctc tttgtaccac ttttaattgg ctatcatcaa attcaagtac atatagagta
    29521 ttagatataa taggagatgt agcattttac tttatgccta taatacttgc aataaatgca
    29581 gctaaaaaat ttaatgttaa tacatcaata gcagttatag tagtaggtgt gtttttacat
    29641 ccaaatttct ctgcatgggt atcaagtggg gaccctatat catttatagg agtaccaatt
    29701 caaggtgtta tatatgcagc atctgttatc cctgcacttt taacagtatg gatgatgtca
    29761 tatatagaaa aattcattaa taaattaaca cctagcatgt taaaaacaat tttaaatcca
    29821 actttagtat tattaataag tgcgccaata gcattaatag taattggacc aataggaaat
    29881 ttcttaggag agggattagc tagtataata aatttactac aaggaaggtt aggatttata
    29941 atggtttgtt tattagcagc tgcaatgcca tttatcgtta tgacagggat gcatcatgca
    30001 ttgactccta tattcatatc agcatttgca actacaggac aagaatcgct aatattaata
    30061 tctcaagttt gtgctaattt agcgcaaggt ggcgcgacat tagctgtagc attaagaagc
    30121 aaagatagta agatgaaaca gttagcatca gctgcatgga tatcagctat catgggtata
    30181 actgaaccag ctctttatgg agttacattg aaacttaaaa aacctgctat gttagcatct
    30241 ataggagcag gaatcgcagg gttatttgca ggaattactc atgtgacttt atatgttcca
    30301 caaaatagta taatggcagt acttggatta tcaggggaaa agggaatttc aaatatagta
    30361 aatggactta tagttatatt tatagcattg gttgctccat ttattatgac atatgttttt
    30421 ggagttgaaa aaaatgttaa ggaaagtaca gataatgtag tagaggaagt ataatattaa
    30481 ctatttacat taatagaatt tttattatta ttgactataa cttgttttgt atttatattg
    30541 atgcaaagaa ataagttaat attaatgata ataattatta taactgacaa aaagcaacac
    30601 aaaagattga tgaagtatat ggaaataaaa agttgacaaa atatgtctga tgttttatca
    30661 tattaattgg aattaggtag taattgttta agtacaagct aagcttattc attaataata
    30721 gcagtatcct aggaaggagt atgcccatta ttatggataa gcttttttta tgcttaaaag
    30781 gaggataatc atgataatta ataagatatt aaacaataat gttgtaatca cattagatga
    30841 taatgatgaa gaagttatcg ttatgggtaa aggaattggt tatcaaaaat caaaaggcaa
    30901 tctaatagat aaaactaaag taaataaagt atttagaata tcaaataaag aaatatcaaa
    30961 caaactacaa gaattactca ataatattcc tatagaacac atgaaattat caagtgaaat
    31021 tatagaatac gcacaaataa agttaaacaa gaagctaaat gaaagtattt acatatcctt
    31081 aagtgaccat acatattctg caattcaaag aatgaaagaa ggaataaatg taaaaaatgc
    31141 aattctttgg gaaattaaaa gattttataa agaagagttt gaaattggaa tgaaagcact
    31201 agatattatt gaaaacaaaa caggtataaa attaccagaa gatgaggctg gatttatagc
    31261 ctttcatata gtgaatgctc agttaagtga aggtcatact ttggcatctg atattacgaa
    31321 gttaatacaa gaagtactta gcatagttag gtatcatttt aggatagaat ttcatgaaga
    31381 atcagtgttt tattatagat ttataatgca tttaaagttt tttgcgcaaa gattgctttt
    31441 agatagtgca catgaaggtg agacagacaa agaattacta agcattatta aatctaaata
    31501 taataaagag tttgaatgtg tagttaaaat taaaaatttc attaaaaaac aatataacta
    31561 catattaaca gatgatgagc ttatatattt aacaatacat ttagctaaag tagttaaaga
    31621 ctcaaatatg taagtgaaat aatttttcag tagtatatta agaatgattg aaaggagtga
    31681 tactaacaaa attataccta tggttggatg gtaacattga gttggccaaa ccttcaaatt
    31741 tcaaaaaatt aatttaaacg aaatatggag gagaaaaatg ggaaaataca aacaattagc
    31801 acaagagata gtaaacaatg ttggtggaaa agaaaatatt agtggattag tacactgtat
    31861 tacaagatta cgttttactt taaaagatga aagtatagca aatgacaatg tattaaaaaa
    31921 catggaaggt attgttactg taatgaaaag tggaggacaa tatcaagtag ttataggaaa
    31981 tcatgttgaa gctgtctata aagatgtagt tgaaataatt ggtttagatg attcaagtac
    32041 aagttcagaa actaaaaaat caggtaatat actagataaa ggtatagata taatatcagg
    32101 aatttttcag ccagtgttag gaataatggc agcttgtggt atgctgaaag gatttaatgc
    32161 attatttgta gcaatgggat tatatagtga tgtatctggt ggttatatgg taataaatgc
    32221 agcaggagat gcattattta cattccttcc tttgttctta ggatatactt cagctaagaa
    32281 gtttggatta aaaccaatgt tgggattagc actaggagct gcagtatgtt atcctgctat
    32341 acaaggtagc agtatatcag caggagcaga tgtgatgtac actttattta aaggaacaat
    32401 gtttgcatca ccagtatata ttgatttctt tggactacca attgtatcaa ttgattatac
    32461 tggaacagtc gtaccaatta tatttatagt gtattttgct tctaggtgtg aaaaattatt
    32521 taataagttt gttccagatt tagttaagtt tttctttgtg ccaatgctta cattattaat
    32581 aacagtgcct gttgcactta tagttatagg tcctatcgca acatttggtt ctacacttat
    32641 ttcacaaatt gtaatttcaa taagagattt tagtccacta ttggcaggtg ctatagtagg
    32701 gtttacttgg caaattcttg ttatttttgg tatgcactgg ggatttattc ctgtatatat
    32761 aaacaatgtt atgactttag gatatgataa tgttatgatg ccattctttg cttgtacatt
    32821 tgcaagttcg gcagttgtgt tggcaatatt tttcaagact aaagataaaa aactaaaaga
    32881 gatgacaata ccaaacttta tatctggtat atttggagtt acagagccag caatttatgg
    32941 aatattattg cctttaaaga aaccatttat aataagttgt attgctagtg ggattggagg
    33001 cgctttctac ggattcttta atcttagaaa atttataact ggaggtatgg gaatatttga
    33061 acttccagca atgattgaac cagatggggg aatgggcaac ttaatagtag ctctttctgg
    33121 tatagctata tcaatggtcg tagcttttgt attaactatg attttatata aagatgagaa
    33181 agtagaagag tctaaaaaag taagaaataa ggggaaagaa gaaataaaag aagtcaagtc
    33241 tactaaatta gaaagagaaa tagtagcaag tcctataaaa ggtgaagtat taaaattaag
    33301 tgatatagag gatgcagcat ttgcatctgg tgtactaggt caaggggtgg caataatacc
    33361 tagtgatgga aaagtagttg ctccagtaga tggtgtagtt acaaccttat ttcctagtct
    33421 acatgctata gggattcttt ctgatacagg tgttgaagtg cttattcata ttggattaaa
    33481 tactattcaa ttagaaggga gaggttttga ggcatgtata aaacaaggtg atagaattac
    33541 aaaaggtcaa actatattaa actttgatgt tgatgcaata aaagagttgg ggtatagtac
    33601 tgtaactcca attgttatta caaattcatc acaattttta gatgtagtag aaacagaaag
    33661 taaaaatatt gaattagaag ataatctaat tacagtctta ttttagtaaa aattgaggca
    33721 ttagcataag ctagtgcttt ggttataatt taaggaggct ttgactatga gttttaagga
    33781 tgatttttta tggggaggag ctactgcagc caatcaatgt gaaggtggat acaatgaaga
    33841 cgaaagagga ttggcaaatg tagatgtatg tccaacagga aaagatagaa cagcagttat
    33901 tacagggaaa ctaaaaatgt ttgattttga tgatgagcat tattatccag ctaaaactgg
    33961 tattgatatg tatcataatt ataaagaaga tataaagtta tttgctgaaa tgggatttaa
    34021 agtatataga atgagcattg catggagtcg aatatttcca aaaggtgatg aagaaacacc
    34081 aaatgaaaag ggacttcaat tttatgaaaa tatatttaag gagtgtaaaa aatataaaat
    34141 agagccttta gtcactatta cacactttga ttgtccaatg catttagtta aaaagtatgg
    34201 tgcatggcgt aatcgaaaaa tgataggttt ctatgagaaa ttatgcaatg ttatctttag
    34261 aagatataga ggattagtga agtactggct aacctttaat gaaataaata tgattttaca
    34321 tgctccattt atgggtgctg gtatttgctt tgaagaaggt gaagatgttg aaaagataaa
    34381 atatcaagct gctcatcatg agcttgtagc tagtgcaatt gcaactaaaa tagcacatga
    34441 agttgattca aataacttag ttggatgtat gtttgcagct ggaagtgtat atccttatag
    34501 ttgtaatcct aatgatgtat gggaagctac aaagttagat agagaaaatt acttctttgt
    34561 ggatgtacaa tctaaaggta aatatcctaa ctatgcgtta aagtatatgg agcaaaaagg
    34621 tattacacca gaaatggaac ctggtgatat tgaattattg aataaatata cagtagattt
    34681 tatttcattt tcatattaca atagccgttg tgttagaact gatgaaaatg cagatgatat
    34741 ggctgaagga aacatattta catctgctaa aaatccatat ctttcataca gtcaatgggg
    34801 atggccaatt gacccattag gtttgagaat tacactaaat catgtatatg atagatatga
    34861 aaagccacta tttattgtag aaaatggatt aggtgctaaa gatatagctg atgaaaatgg
    34921 ttatgtagaa gatgattaca gaattaatta tctaagagaa catataaaag ctatgcatga
    34981 tgcagtttct atagatgggg tcgatttatt aggttatact acatgggcac ctattgattt
    35041 agtaagtgct ggtacaggtg aaatggaaaa gcgttatgga tttatatatg tagatagaga
    35101 taatagtggt aatggaagtc taagaagaat gaaaaagaaa agttttgaat ggtataagaa
    35161 agtaattgct tcaaatggtg aagatttata ttaaacaaga aatctatgtt aaataagaaa
    35221 tttgtattaa ataagaagag tataaaaatt aataataagt ataattttta acttttttaa
    35281 ctatagaaaa tcaatacaga attattttac attgtattta tgcaatagta tgattcacaa
    35341 ataaaatttt agaaaataaa attgttaaga agtatgggaa gtaagtgcaa tttactttgt
    35401 aataaaagat gaaaaaggcg agcttaggtt ttaagcttgt cttttttatt atgtataaac
    35461 tatgatactt taaaattcta tgaaaaattt atttaataga gaaattaaaa atttaatggt
    35521 aatcaaattc tttaccaatg aatataaaaa atgtctacgt gttataatag tttaaactaa
    35581 taaacgaaag gtagatgaat gaatatgaaa taccaacata taatagaagt tgacaaagaa
    35641 ctttgcattg gatgtgggct ttgtaaaaat gattgtccag taaacaatat aattatagaa
    35701 aataaaaagt cagtaattaa aaaacaagat tgtctaatgt gtggacattg tgcagcaata
    35761 tgtcccacaa aagctattac cttgacagga tttgatgaac ctcctataga acttacaaat
    35821 aagccaaagc tagattcaga tgaattacta atggcaatta aatctagaag aagtatacgt
    35881 aaatttaaag acaaagaagt ttcttcagaa attgtaaagc aaattattga ggcaggtagg
    35941 tataccccta gtgctaaaaa ttcacaagat gtatcatata ttgttttaga caataaaaag
    36001 tcaatttatg aaaacgaagc agtgaaattt tttagaaaaa taaaacctat tgcaaatata
    36061 gctattaaat attcaaaaga agttgagatt gacgataact ttttctttaa gcatgctcct
    36121 attgcaataa tgataattac aaaagataag attagtggtt cacttgcagc atcaaatatg
    36181 gcattaatgg cagagtctta tggtctaggt gttttattta gtggatattt ttcagatgta
    36241 gcaaataatt cgccaaaatt aaaaaagctt ttaggtctaa aacgcagtaa tcatgttctt
    36301 acaacattag taattggata cccagatgtt aaatatagac gtactgcaca gaaagaagta
    36361 gcaactgtta gatacttata agagagggtg gattttatgg aaaaagaatg tcaacaaaga
    36421 atgcaagaaa taacatctaa atttaaagaa tgtcgcaaag ctatctctgc tatgggtgat
    36481 gagactagac aattgatttt aattgctctc ttagagagtg attttaatgg tatacgagtg
    36541 ggagaaatca caaaaaaaac acatctttct cgtcctgctg tatctcatca tcttaagata
    36601 ttaaaagaag cagagattgt taatttgcgt agagaaggaa cgaaaaatta ttattatctt
    36661 gattcaaaaa aatcacaatg gaagtcacta acagagctaa taaatcttat atttgttgga
    36721 atacaacata ttagtgaaaa tgatataaga aaaggtgatt aaattatact tgatttaata
    36781 caaaaagatt gatttaggaa ctaaaaagtt cactttgaga cactatcttt tgaactatac
    36841 ccatcttagg agacagttaa aatatgtaaa actgtataaa acaatgggta tagttcattt
    36901 tataatggtt taaatattaa gaatatatta ttctaaatag tatctttctc tgttttgagt
    36961 tttattttta tactcaatag cttcagtggg gcaaccacaa atacatgcca tacaatgagt
    37021 acagctattt ttccaaactg gctttttgtt ttttaaatta atattattta aaggacatag
    37081 ctctacacat tttccacagc caatgcattt atcagtataa tgaaatcctt tggcactaat
    37141 aattgtctta taaaaaactg tatttattat accactttta attgtacctc ctaagccact
    37201 aggagtaata gtttcaaatt cattttcatt gcgtatatca gaaataatcc tatatatttg
    37261 tttatctgct tctttaatca tttgttttga tgtttctttg tctgttgtag aaaataaagc
    37321 tatataattc tctggcattt taatttctgc catacctttt aattgccatt ccttgtattt
    37381 acatagtttt tgaatatagt ttatggattt tgctgtatct cccccacaag tcattacaaa
    37441 gtaaactctg ttgtctccta gaaattttgt ttcttttata aaatctgtaa ccactattgg
    37501 caatctccat gcataagtag gacatacgaa tatgaatggt ttacctgctg atattaattc
    37561 atctgttttt tcctctttta taagttgatt tagtgatatc agttcatcat ttaattcttg
    37621 tgcaatttta cgagccacat atcggctatt accagtaccg ctaaaatata aaatcataat
    37681 aatatctccc taactatttt ttattttaac tcttaatttt ttatttatta tagtacttta
    37741 ttgaaatatt ctattcaaat gtatgtaaaa tttagtagtt aagaaaatta taacacatta
    37801 gtaattttta tattcattgg taaagaattt gattaccatt aaatttagta acaaaatatc
    37861 aactggattt ttatataaga agagatagta attttcatgt gaaagattgt aaatataaag
    37921 atgattttga agggcaataa gaagataaaa ttgatagctt aataaaaaat atttaacttt
    37981 tatattaact gttttaaata ttataattat ctatcttgaa taatcaacca tagaagactg
    38041 tatacaaaga caaagttgtg tacagtcttt tttataaatt taatgtctat aattaaatac
    38101 actattaaaa tcaataagtt attatttcaa agtagtatta tccaaatttt tatgatggtt
    38161 aatacaattt gttagccatt ttatagcacc ttgttccctg ataatagctc catcaagaac
    38221 tatataatca ccaaaatcat tactatcaag aggaggaact tcactataac gctccatttg
    38281 actgtacaag aacgtaattc tttcttggtg ttgaagcaat tgatgctcta gtaaaataag
    38341 cctagtatct atatctaaat tattagaaaa atacattcta agacgaaaaa tatccttagg
    38401 ggttctttcc attggctcat ccttttcaag ccatttcaag aactcatctt tacctttttc
    38461 tgtgattgtg tataactttt tctctaatac atcaccacta attttaatat catatacaat
    38521 aagcttttca tttaccaatt tttttagttc aggatatatt tgactatgct ttgcattcca
    38581 aaactcagct aattgaaaat tgaactcctt tacgatatca taaccagtca taggatttct
    38641 atttaataat cctaaaattg catactttaa agttcgcata aatacacctc tttctttaat
    38701 tacatactta ttataacacg atatatttag tttgaattaa ttaatatagc tatgttttaa
    38761 taagaacttt taaattatga ggaacaaaaa ataaataaaa ctatatataa aaatgaaatt
    38821 atgtaaatat aaacatgtaa gtatttacat gtttatattt acatatagaa aaaattgtga
    38881 taatcttaac taagaaattc tatttaataa atttatcgag gaggagagta atgaatttaa
    38941 cacaaaaaag atggattata ttaattgcaa gttgtttcat caatttatgt attggttcaa
    39001 tatatgcgtg gagtgtattt gcatcaccta tggcagaaca tttaaatagt cttgctggaa
    39061 caacttttac agctgctagt ttagcaattg tatttacagt tacaaactca gttggaccta
    39121 ttactatgat tacaggggga atgataaatg ataaatttgg tcctaaaaaa gttgtatttg
    39181 taggaggatt aatttttggt ataggtatga tattggcagg tttctcaaaa ggattgccta
    39241 tgttgatttt atcatatggg ataataacag gtcttggggt tggcatggtt tatggatgta
    39301 caataagtaa ttcaattaaa ttttttccag ataaaagtgg attagttggt ggtataacaa
    39361 cagcagctta tgggtttagt tcagtaataa tgcctccagt tgctaattgg ataattaata
    39421 aatatggagt aacttctgca tttaaatcaa taggtttagt atttcttata attgtatgtg
    39481 tttcttcttt ctttatcaaa aaatgtccag cagatttcac accagaagga tgggtaccac
    39541 cagagaaaaa aggaaaagta agagttgtta ttaataaaaa ttggaaagaa atgctttcaa
    39601 cacctatatt ttatctaatg attataatat taacttgtgg agctttctat ggattaatgt
    39661 gtgtgtcatt agcatcttct ctagcaacaa atatgattgg gatgacaagt tcagattcag
    39721 caatagtggt ttcaatttta gccttgttta atacattagg ccgtattgtt gcaggatata
    39781 tatcagataa aattggtaga ataaatactt tatcaggtgc attagttatt tcaatttttg
    39841 gtttattatg tttgttattc tcaagagaag gagatgtagc aacattctat attggaataa
    39901 taattatagg tatatgtttt ggttcattta tgggaatttt cccaggattt acagtagata
    39961 aatttggacc taaaaataat agtgtaaatt atggaatcat gtttattggt tttgcttttg
    40021 caggatatat tggacctgca gtgatgaaaa atgtatattc tgtagatggc aattatcaaa
    40081 gggctttcat tatagctata attatagctt taataggttt tatattaaca tttttatata
    40141 aatatataaa taaaatacaa aatttaaaga tagaaaattt aataaaggga gatataaaaa
    40201 atggaaagaa aatatccaaa tctatgtaag ccaattaaaa ttggaaatgt aacattcaaa
    40261 aacagaatgt tttcagcacc tatgggggga acagacataa cagcagattg tacaattggc
    40321 tcaaaatcaa cagcctttta cgaattaaga gcaaaaggtg gagcaggtgc agttacaatt
    40381 agtgaatgta tggttcatcc agaaacagat ggttcacatg catatcatct agacttaaaa
    40441 atagttgatt ccttagcaag ttttacttat acagcagatg caattcgccg tcatggagca
    40501 ataccaagtg tagaactatc tcattcagga caatattcag gaacttattt agttgacaaa
    40561 gataagaagc gaggattatc acaatgggga gtaagtccta gtgttcgtcc tgatggatta
    40621 gagataaaag aacttacaga agaaataatt gctgaaatag tagagtcata tggaaatgtt
    40681 gcagcattgg ctaagagagc agggtttgag atgattatga ttcatggtgg acatggctgg
    40741 ctaatcaatc aattcttatc accttacttt aataaaagaa ctgacaaata tggtggttct
    40801 ttagaaaaca gagttcgttt tgctaaagaa gttttagata gtgttcgtaa agcagtaggt
    40861 ccaggatttc caatagagtt taggatgagt ggttctgaat tatttgaggg agggtatgat
    40921 ttagattatg gaacagaaat tgctaaatta ttggaatcaa gagtagattt acttcatgta
    40981 tcagcaggta cataccaaag agggtttgca gtaacacatc cttctatgtt cttagaacat
    41041 ggatgtaacg tatatcttgc agaagaaatt aaaaaacatg taaatattcc agttgctact
    41101 ataggagcat taaatgaccc agaaatgatg gaagagataa ttgctagtgg aaaagcagat
    41161 gttatttaca tggctcgtgc attactagct gaccacgaat taccaagaaa agttatgagt
    41221 aatcaagatg aaaaaatagt gaaatgccta agatgcttca cttgtatggc agaacgtgct
    41281 accacttcta caagacgctg cactgttaat ccacttattg gacgtgaatt agatggaact
    41341 gaagttgttt cagtattaaa acctaaaaaa gtattaatag caggtggtgg accaggagga
    41401 ttgcaagcag ccattacagc tgtaaagcgt ggacataaag taatactttg tgaaaaaact
    41461 aatgaattag gtggaatttt aaagggagaa caagcacttc catttaagta taaaatgtat
    41521 gaattaggaa atacttttgg aaaaatagca aaagatttag gtgttgaagt acgtttaaat
    41581 acaacagtta caaaagaata tgttgaaaat gaaaatgtcg atgcattaat tatagcagta
    41641 gggtctgaac ctcttgttcc accaatagag ggtcttgatg gagaaaatgt agtaatagtt
    41701 aatgattatt acgtagaaaa agaaaaagtc actgacgaag taatagtttt aggtggagga
    41761 ttagcaggat gtgaggctgc aattcactta gctcaagaag agaagactgt tcacttagta
    41821 gagatgagag cagaacttgc cccagatgca aatataaggc atcgtccaat attattagaa
    41881 gaaatagaaa gacaaggtat atatgtatat acagaacata agggtttaag tgtaacaaca
    41941 gaaggtgtag tatgtatgaa taaatctggg aataaaataa atattccagg aacctcagta
    42001 atatgtgcac ttggacaaaa accaagaaga gaagtggtag acactttact tgattctgct
    42061 ccatatgttg ctcaaattgg agactgtgtt cgtgcatcta caataacaaa tgcagtatac
    42121 caagggcatc atgctgcgct agatatatag ttaaaaagac gtagatagta ttgttttatt
    42181 ataaaaaagg agtgtctcaa aatgaacttt ttagttcatg agacactttt ttacatgaaa
    42241 ttaaatatat ttttatcatt tcaacaatga aaaaagatta tctttatttt gagacagcac
    42301 ttttgttatt atgattttaa ttcttattgc aagttttcat ttgattgatg agtaaaactt
    42361 tttaagatgt agaagtattt aacaatgtaa atgaccctaa cttaaatact ttagcgaagg
    42421 ttgacagatt tgtgtttgct tttatctatt ataacttaag gaaacatgtt acctgaacaa
    42481 taaatttata taataaaagt atataaatat atatatgaat gttaagatta gttgatagaa
    42541 tttaaataat aaatccaata ttataaacat aggaggtatg aaaaatatga ttaatgagaa
    42601 tttaaaaagt ctaagaaaaa tccaccagta tacacaagaa gaattagctg aaaaacttaa
    42661 tgtttctaga caatcaatag caaaatggga aagtggagtt tcccaaacaa aaggatatta
    42721 ctgaaataac ctgtgtttac ctgcttgtag agaaaagaat attgctatta agccagagat
    42781 actacataaa tgtgttggta tctctttttc gttaatgcaa cacagtaaat tatttcattt
    42841 tcctaattat tttagactga taagtaactt tttaaatgaa gaaaagaaat gacaatactt
    42901 aaaggagcat ggtatactta aaggagaatt tcattaagga ggaaaaagag attgtttaat
    42961 ctaatagaaa tgttatttcc tataatgttt atactgattt ttataataat tgttttcact
    43021 tttgtgaaag gaatagctac ttggtttaaa aacaataatt ctcctcgctt aactgtgtca
    43081 gcaagaatag tagcaaaacg tcagaatacc actcataaca atcaaccaaa cgctggtgat
    43141 atatcaggag cacatggatt tcatacaatt tccagcacct cgtattatgt gacatttcaa
    43201 gtagatagtg gtgatagaat agagctgtct gtatctggct cagagtatgg agtgcttgca
    43261 gagggagata aaggacagct gacattccaa ggcacaagat atttaacatt tgatagggaa
    43321 aattgaattt agaggtgcat tatgaataag tttttgagtc agataaggcg tatagataat
    43381 gaaatatcta ttactaggaa aattataaat acaatattta tactgtgttt tggaatagtt
    43441 ttaggaactt ttgcaaaatt tttagatact acagcaagca atactttacc atttatattt
    43501 gaatatttag atattagtaa tttttttggt cgttttgcta tatggttatt gattgctttg
    43561 tacattgcaa tttatagtca ttcttccata agagcatcat taaatgtgtt agtatttttc
    43621 attggaatgg tttcaagtta ttacctatat tcgtattttg ttgctggatt ttttccaaaa
    43681 aattatgcaa tgatttggct tggttttact gtcatttctc cattgttagc ttttatttgt
    43741 tggtatgcta aggggaaaag caaaatatct tttatacttt cagtaataat aattgcaatt
    43801 ttatttaatt ttacatttat ttatggttgg atttattttg atgtgtattc aattttagaa
    43861 gtgatagtct ttggttgtgc attaatagct ttaaaacgta acacctttag ggagacaacg
    43921 tatatgatat tgagtgcagt tgttattgct gttatactta atatgttagt tccatttcat
    43981 tttggttagg cagattttaa gattattata gatagaatat aaatttcacg aaccgccgaa
    44041 gcgatccgcc agcggtggcg gtcgtaagac atatcgccac gccagcttgc tggcgaggtg
    44101 gcggttcact ttggcggttt ggcgagcctg cgagctttct aagcccccgt tatctaacag
    44161 ggggggacta ttacaaaaga caagagcaaa aacaaatata aaactgctga tatgcaacga
    44221 tttataaggt tgtaagaact tatttcactt tacaccctat tctttctacc cgacaggtct
    44281 aaaacagcat atcaatagaa aataaatata agatacaagg tgtatgctcc tatgagtata
    44341 tgcctttttt tgattggagg aatgaagcat gaatgatatg aaatttattg aaacattaaa
    44401 gcaaaagaga aatgtctgtg attattcgca atcacgactg gcacaggaac tacaaatcaa
    44461 caggcagaac ttaaatgaaa ttgaaaacgg aaaaacaaaa gccagtaaag aaatgaaaca
    44521 tatactctta cattatcttg attattgtaa ttgtacacag cctttcactt taacgattga
    44581 ctatctgcgt gttcgttttc ctaccacaga cgcattggaa attataaaaa atgtgctggc
    44641 aatgaaaagc gaatatttta tacatgaaga ttacggaatg tatgcttatg aagaacagta
    44701 tatctatggg gatattagtg taaatgcttc taaagatagt tctatgggtg ttttattgga
    44761 attaagaggt atggggtgtc gggacttaga atatgtttta caggtaagag ggatagactg
    44821 gtattgcttt ttaagatgtt gtatagatta tcaaggtgtt tttaaacgca tagatttagc
    44881 agtcaatgat atgggcggat tgctggatat agaaattcta agggaacgtt attatgctaa
    44941 caaggtatgg aaacgttcaa gaacccatga agcagtagac agcggaaaat tatcgggtac
    45001 acatggagat actgcaaaaa ctttttatat cggttcaaag aatagttcta tctacttttg
    45061 cttgtatgaa aaagaaaagg aacaaaaaag caagggtata aaaacagata ttaagaaccg
    45121 cttcgaaatt cgtttaaaaa atggaaaagc aggacaaacg gtagagcaac ttgttttttc
    45181 aagaaatccc gaacagacca tagcaagctt tattctcaca cagatagatt ttcccgatta
    45241 tattttatgg gatatatttt tagataatgt aacgacctca cttccgttta ttatgacacc
    45301 tgttgctgtt aatatggata aaataaaacg ctggctagaa agacaggtca tgccctcttt
    45361 attgatgata aaagagattg aaaagaaaac aggagcaaat tatctggaag aaatcgacag
    45421 gcatacaagg ctaacagaaa agcagtaatt aaagattaaa caaatgacaa cagacatagc
    45481 agatatgatt gagaaagata ccgctgttcc acaagggaat gacggtattt tttaatttct
    45541 tcaaaaaaac tgcaacaaaa tgactgcttg tgtacagata tggtgcgagg aaaggaaatc
    45601 taactttttt tgaaaacagg tcattaaatc ggtggctgct gtccctatat gatgtgaaag
    45661 aagaaacgat ttttaatttc aacttaacat ttgacagttt tcattcccta tatggagcaa
    45721 agaaaatctt ttattccata gttggcagtt acggtgttgc atgggtagtt agggtgtgaa
    45781 aagaaaaaca gatttttttc atcaccaact tagcaaactg atacttgctt tccctatatg
    45841 gtgcaggaaa agagagttta actattttca tttcaactta gcaactggac gtttgctgta
    45901 cagatatgta gtgaaaagag aaaaagtttt gaatattggt tacgcttcgg ctcttttcca
    45961 acgcggtata taggaaagac gattttcttc tttcatgttc tttgacaact gaataaagca
    46021 attcaatacg tttgacagac agcgatccat tgaagcagat acgccatgac cttttccctg
    46081 caaaagcgag cgaaccctat ttgtgttcct gtaaaagact tgtcctcttt tatcgccatg
    46141 acctgttctg tctgataaat gatactcccg tacagccaca gcttgaacgt tcagagcgtc
    46201 gcacgcaatg ggtacggcta cataagaact atgcagaggt ggaattcctg tgggctatga
    46261 caaatgccgt tgaattgctt agaaaaaaga ttatggaaag ggggtatgtg ttattgctga
    46321 tattgtaaaa acaatgcaag aaaagacaaa taataagcgt agcattggca acagaggaaa
    46381 aacaaagatt gttgtaaaag agcatttttc cgaaaaggga aagacaatgg aggaacttct
    46441 gactgatgtt atgctggaaa aagcaaagca gacaatcgca taaaattggt gcagttgtaa
    46501 gacatagatt gaatggcaaa aagtacccgt gttatacttt acttgcaagc aggtattgta
    46561 aacacgggtt agttataaag gaggataact gacaatgaaa caacagattt acaatactgc
    46621 actttatctt aggttaagcc gagatgatga attacagggc gaaagttcca gcattaccac
    46681 acaaagaagt atgttgcgtc tatatgcaaa agaacatcat ttgaatgtga ttgatgaata
    46741 tattgatgac ggctggtcgg gaacaaattt tgaaagaccg agttttcaaa gaatgattga
    46801 ggatatagag gcaggaaaaa tcaactgtgt tgtaacgaaa gacctttccc gacttggcag
    46861 gaactatatt atgacaggac aatatacaga attgtatttt cccagccata atgtccgtta
    46921 catagcgatt gacgacggtg tagacagcga gaaaggcgaa agtgagattg caccatttaa
    46981 gaacatcatc aatgaatggg tggcaagaga tacaagccgt aaagtgaaat cggcatttaa
    47041 gacgaaattt gcagagggtg ctcattatgg tgcttatgct ccattaggat ataagaaaca
    47101 ccctgacatc aaaggaaaac tgctggttga tgatgaaaca aaatggattg ttgaaaaaat
    47161 cttttcccta gcctatcaag gttatggtag tgccaaaatc acaaaggtac tgcgagagga
    47221 aaaagttccg acagcgtctt ggctgaattt tacaaggtac ggtacttttg cacatatctt
    47281 tgagggaaag cccgaaagca aacgttatga gtggacgatt gctcatgtca aggcaatctt
    47341 aaaaagtgag gtttatatag gcaacagcgt tcacaatatg cagtctacgg tttcattcaa
    47401 gagcaagaag aaagtgcgta agcccgaaag tgaatggttt cgagtggaaa atacccatga
    47461 accgattatt gacaaagaag tgttctatcg tgtgcaggaa cagataaaat caagacgtag
    47521 acagacaaag gaaaaagcaa caccgatatt tgcagggctt gtcaagtgtg cggattgtgg
    47581 ctggtctatg cggtttgcaa cgaatacggc aaataaaacg ccttacagtt attatgcttg
    47641 cagttactac gggcagtttg gcaaaggtac ttgttctatg cactatatcc gttatgatgt
    47701 gttgtatcaa gccgtattga aacgattgca gtattgggct aaggcagtac agcaggacga
    47761 agaaaaggtg ctgaacaaga tacagaaagc aggcaatgca gagcgaatac gggaaaagaa
    47821 gaaaaaggca agtgccttta agaaagccga gaaccgacaa aatgaaattg accgtttgtt
    47881 tgcgaaaatg tatgaagata gagcctgtga gaagataaca gagcgaaatt ttgtgatgtt
    47941 gtctagcaag taccaaaaag aacaaataga actggaacag cagataacca acctaagaga
    48001 agaattaagt aaaatggaac aggatatgat aggtgctgaa aagtggattg agttaatcaa
    48061 ggagtattcc gtaccaaagg aactgacagc accgttatta aatgcaatga tagaaaaaat
    48121 ccttattcat gaagcaataa cgaatgagga taacgaaaga atacaggaaa tagagatata
    48181 ttaccgattt attggaaaag tagactgata aagagggttc atatctttaa ctaagggaaa
    48241 ctggagaatc tattcctgat ataggtagtt gcattaaact tgcaaaattg tacaaggtca
    48301 aactagatga cttagttaat cattcggaag aaaaaacagg tattatagta cctcctaaag
    48361 gaaagttttt ctttggagca gtagtagttg gtgaacgtgg tcagattgtc attccaaaag
    48421 aagcaagaga agtatttaat ataaatgcag gagataaatt attggtactt ggagatgagg
    48481 aaagaggatt aggaattgtt catcaacgtg atttgataaa ttttataggt catatgggag
    48541 tagtacgtaa agaagaatga ttttaaaaaa ttataaaagg aggtatgaaa tgtgagtaaa
    48601 atagtatttt ttagcattct tgcatatggt catactaatc ctactattga agtagttcgt
    48661 gaattagtgg atagaggaaa tgaggtattg tattattctt ttaatgaatt taaagataaa
    48721 attgaaggtg ctggagcaaa atttatttgt tgtgataaat accttccaga attactccct
    48781 ggagatgaga aaaaaattgg taaggatttt cctggattaa ttgagatgat agtagatact
    48841 actatttctt tagatgaaaa agtttgtagg gaacttaaag attttaatcc agattgcatt
    48901 gtatcagatt cattaagttt ttggggaaag ttatttgcac aaaaacttaa tattccatat
    48961 gtatgctcta caacaacttt tgcatttaat aaacatacag caaagttgat gaaacaaaac
    49021 tttatagaaa ttatacgtat gatttttggt gtaagacgca ttaataaaaa aataaagctt
    49081 ttacaaagaa aagggtatga agtaaaaaat tttatttcta tagtttctaa tgataatgat
    49141 acggatacca ttgtatatac atcaaaagaa tttcaaccaa tggtagaaac cttctctagt
    49201 aagtattcat ttgtaggtcc atcagtatca aagttgatta ttaaacctaa agagagaaaa
    49261 agaaagttaa tatatatttc gcttggtaca attaataata aaaatgttag cttttataaa
    49321 aactgtataa atgcatttaa agattctgat gttgatgtga ttatgtctgt tggtagaagt
    49381 actgatatta aaagtttagg aaatatacca aataattttg aggttaaaaa tagtgttgaa
    49441 cagattgcta tacttcaaaa aactgatgtg tttattactc attctgggat gaatagtgtt
    49501 aatgaaagtt tatattatgg agtacccatg gtattatttc ctcaacattc ggagcagaga
    49561 atggtagcta agagagttgt tcatttggga gcaggaatta tgttaaaaga ggataagtcc
    49621 gaaagtatta aaaaagcagt atttcaagtt ataaatgaca atgaatataa agaaaatgca
    49681 actaaactat caaaaagctt ttataatgca ggaggctcaa aaaaagcagc agatgtaata
    49741 ttacagatta tatgtaatag tagacaagtt gacatataat agatttgtta aaaatatata
    49801 tacatacatg caatttaatc aaagtactga cagtggggtc ttttaaccta ccagttgggg
    49861 catatatatt gatgctaaat atgagggatg ttatatttta ataaaaaaga taacatcctt
    49921 tttttagtgc gttatatcaa gttttaaata ataaatgaag gaggattaat atgaaaaaat
    49981 ataaagttat ttttatagta ttaattatag gtattataag tgtaacaggg tttgttgtat
    50041 ataaaaactg cattaaacca tggaaagatg ctgaaacata tattgataag tatatggtaa
    50101 aacaagatat ttcaaaagat aatataaaaa gtattacaaa agaaaaagcc aagaaggcaa
    50161 gttacgaagg aatactatac aaagtgtatt acaaagatga ccctcgatat gagtatcaat
    50221 atttttatag tgataattat catgctctat atgtaaataa agtaatgtta caaatttatg
    50281 atgtggaaaa taataataaa cttttaagaa gtaatgagga attaaaaagt gtaaagtatc
    50341 caccaatata tcttaaatat aagccagata aatgatttat gacaaaatag aatgattttt
    50401 attaaaattt atttaaaaat atcactagag tgtttaa
//
LOCUS       DAELXX010000015        47855 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.015, whole genome shotgun sequence.
ACCESSION   DAELXX010000015 DAELXX010000000
VERSION     DAELXX010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 47855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47855
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.015"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..393
                     /locus_tag="KPH29_000845"
     CDS             <1..393
                     /locus_tag="KPH29_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466472.1"
                     /translation="VKGFNIDKIDFVYSVKPIDVFLKTFPTLLWYTTFPLMILTLTKN
                     RFTTIAILITYTFADIFFILHMYPFVSMINVNGFYIQKMFYMPNNIDVRFNELKNYFF
                     INRGSLVVISIIIVFYIARKSTVLKKSK"
     gene            complement(489..1322)
                     /locus_tag="KPH29_000846"
     CDS             complement(489..1322)
                     /locus_tag="KPH29_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HBG1466473.1"
                     /translation="MNVENQSLSDIEIILSHRYDNGADYWTTPDKRLIKGSPFSTLDS
                     VLYLLELGVNPDDTLLKECADLIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGY
                     ASDIRMQKTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLN
                     KESKLDKAVDFLLDHWTIKKPLGPCHYGIGTLFMQVEYPFRNYNLFLYVYVLSFYNRA
                     KKDKRFLEALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYEILKNLE
                     K"
     gene            complement(1390..2019)
                     /locus_tag="KPH29_000847"
     CDS             complement(1390..2019)
                     /locus_tag="KPH29_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HBG1466474.1"
                     /translation="MDENITKNNYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLNRF
                     TLNFFDRQYIIDYPSGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIP
                     GGNVYYPNFLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMI
                     FILWFGDDEFPPNSNILFDSNIVYYFNAEDLAVVPDTAINAILNKINQL"
     gene            2330..2989
                     /gene="cprR"
                     /locus_tag="KPH29_000848"
     CDS             2330..2989
                     /gene="cprR"
                     /locus_tag="KPH29_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HBG1466475.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
     gene            3200..4258
                     /locus_tag="KPH29_000849"
     CDS             3200..4258
                     /locus_tag="KPH29_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG1466476.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPKKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
     gene            4509..6653
                     /gene="fdhF"
                     /locus_tag="KPH29_000850"
     CDS             4509..6653
                     /gene="fdhF"
                     /locus_tag="KPH29_000850"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:4923..4925,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HBG1466477.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKTWGVKLSPNNGYSLTQVPDLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            6702..7535
                     /gene="fdhD"
                     /locus_tag="KPH29_000851"
     CDS             6702..7535
                     /gene="fdhD"
                     /locus_tag="KPH29_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBG1466478.1"
                     /translation="MNNIYSKTKLQENSKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYVNTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCASVKSNIKINYDTIYSIMDRNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            7546..7719
                     /locus_tag="KPH29_000852"
     CDS             7546..7719
                     /locus_tag="KPH29_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466479.1"
                     /translation="MCTKKEEQKCSKCEICGVLIDDTDKVYNNTYNLCKKCKRNISTE
                     KIVKYINIYDESK"
     gene            7777..8328
                     /locus_tag="KPH29_000853"
     CDS             7777..8328
                     /locus_tag="KPH29_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG1466480.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRHCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            8353..9729
                     /locus_tag="KPH29_000854"
     CDS             8353..9729
                     /locus_tag="KPH29_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBG1466481.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            9729..10244
                     /locus_tag="KPH29_000855"
     CDS             9729..10244
                     /locus_tag="KPH29_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG1466482.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNPDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(10322..11605)
                     /locus_tag="KPH29_000856"
     CDS             complement(10322..11605)
                     /locus_tag="KPH29_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HBG1466483.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSHSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKKLNKERLDKLMQ"
     gene            11793..12215
                     /locus_tag="KPH29_000857"
     CDS             11793..12215
                     /locus_tag="KPH29_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HBG1466484.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSEKNGENIIRDILDGNISSECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            12317..13255
                     /locus_tag="KPH29_000858"
     CDS             12317..13255
                     /locus_tag="KPH29_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBG1466485.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            13441..15465
                     /gene="ligA"
                     /locus_tag="KPH29_000859"
     CDS             13441..15465
                     /gene="ligA"
                     /locus_tag="KPH29_000859"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBG1466486.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            15625..16974
                     /gene="rph"
                     /locus_tag="KPH29_000860"
     CDS             15625..16974
                     /gene="rph"
                     /locus_tag="KPH29_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBG1466487.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRAIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKTIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEIADEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARAIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNVPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            16974..17444
                     /locus_tag="KPH29_000861"
     CDS             16974..17444
                     /locus_tag="KPH29_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG1466488.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTNMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDDIKIEEIRI"
     gene            17638..18924
                     /locus_tag="KPH29_000862"
     CDS             17638..18924
                     /locus_tag="KPH29_000862"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBG1466489.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            19073..19657
                     /gene="clpP"
                     /locus_tag="KPH29_000863"
     CDS             19073..19657
                     /gene="clpP"
                     /locus_tag="KPH29_000863"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBG1466490.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSQGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            19676..20926
                     /gene="clpX"
                     /locus_tag="KPH29_000864"
     CDS             19676..20926
                     /gene="clpX"
                     /locus_tag="KPH29_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBG1466491.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(20997..21524)
                     /locus_tag="KPH29_000865"
     CDS             complement(20997..21524)
                     /locus_tag="KPH29_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG1466492.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(21512..22024)
                     /locus_tag="KPH29_000866"
     CDS             complement(21512..22024)
                     /locus_tag="KPH29_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG1466493.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            22204..24567
                     /gene="lon"
                     /locus_tag="KPH29_000867"
     CDS             22204..24567
                     /gene="lon"
                     /locus_tag="KPH29_000867"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBG1466494.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFNSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLGNIPRPLLDRMEVIEVSGYIEEEKLNI
                     ARKYLLPKQIKEHALKENFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTAVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            24557..25168
                     /locus_tag="KPH29_000868"
     CDS             24557..25168
                     /locus_tag="KPH29_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBG1466495.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(25433..26803)
                     /locus_tag="KPH29_000869"
     CDS             complement(25433..26803)
                     /locus_tag="KPH29_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG1466496.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYALGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDISGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFIGLILTVFFMRK
                     TDKSIS"
     regulatory      complement(26978..27093)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            27381..28460
                     /locus_tag="KPH29_000870"
     CDS             27381..28460
                     /locus_tag="KPH29_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG1466497.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKATEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     gene            28852..29532
                     /locus_tag="KPH29_000871"
     CDS             28852..29532
                     /locus_tag="KPH29_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBG1466498.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            29525..30904
                     /locus_tag="KPH29_000872"
     CDS             29525..30904
                     /locus_tag="KPH29_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBG1466499.1"
                     /translation="MINIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGVLLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            31075..32160
                     /locus_tag="KPH29_000873"
     CDS             31075..32160
                     /locus_tag="KPH29_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBG1466500.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKVKNLLSIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTNNG
                     IEPPLITRVLMGISTVVTNNLAYIIFSFILFSIGSIYFFITNDNIKKLINSLKFKIPF
                     IKKINQLVATTRFSRALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANVFPKLFISMANTGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPM
                     LIVFMGLIIGTFILAMVVPMFDAITNF"
     gene            32214..32573
                     /locus_tag="KPH29_000874"
     CDS             32214..32573
                     /locus_tag="KPH29_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBG1466501.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
     gene            32626..33498
                     /locus_tag="KPH29_000875"
     CDS             32626..33498
                     /locus_tag="KPH29_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HBG1466502.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKSHKKVFIDKSIIKEINFDIK
                     NDSNSIEKIKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDI
                     LSMIEFEITQYIPININNYSIKYKVINSKNDKLNIQVILFPKSIIQLIKNISENLNMN
                     PKTININFDILQKLINLNLIENFSEEGVFIECKKSQLIINITKDKKIRETYLLSKEGK
                     SYEFIKRLLRDFKYVYYYGIENCFIEEYFKYTDKSIEVNPLRLKDKVKVENNFDADLE
                     DNRINYINSIGMII"
     gene            33499..33942
                     /locus_tag="KPH29_000876"
     CDS             33499..33942
                     /locus_tag="KPH29_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466503.1"
                     /translation="MKVDKTINFLNGFKKTKPCNKIYKIIIVLILTCILFKSFMYVKS
                     INELEDYINQNKLVDKSKHTLPTTDKTREKFSKIKLNEIKKIYETIGINSITKISIDK
                     NKLEMEGVCSDLDVLNNLRKIKYIKYYYIDSIVRDGEYYRFRIKS"
     gene            33957..34541
                     /locus_tag="KPH29_000877"
     CDS             33957..34541
                     /locus_tag="KPH29_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466504.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLTSLKNEKQNI
                     INKINNQDNYQIKDNKHIDDKYIESVELNKNEELKNNFNNSFLDKKTNKDIVLLVENE
                     LRDIVNVEYINQESSLDNHNKKQVNLEIKVNGELANVLKLEGVMKKLRLNNKLEKIEI
                     NKLKVNNFDVQNIPNEYKQEKDNIVECKMTVRVI"
     gene            34794..35831
                     /locus_tag="KPH29_000878"
     CDS             34794..35831
                     /locus_tag="KPH29_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBG1466505.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGANLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLSGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            35912..36277
                     /locus_tag="KPH29_000879"
     CDS             35912..36277
                     /locus_tag="KPH29_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466506.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINVAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            36282..36599
                     /locus_tag="KPH29_000880"
     CDS             36282..36599
                     /locus_tag="KPH29_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466507.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            36647..38251
                     /locus_tag="KPH29_000881"
     CDS             36647..38251
                     /locus_tag="KPH29_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466508.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVVLKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYINVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            38375..40003
                     /locus_tag="KPH29_000882"
     CDS             38375..40003
                     /locus_tag="KPH29_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBG1466509.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTNLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIAKTYRRERLINDLQKAILNYLL
                     KLSKAPLDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNISFSEEGQSELD
                     VMYNRVVSAYTYALESMRTDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            40268..41617
                     /locus_tag="KPH29_000883"
     CDS             40268..41617
                     /locus_tag="KPH29_000883"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBG1466510.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSAEKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            41773..42852
                     /locus_tag="KPH29_000884"
     CDS             41773..42852
                     /locus_tag="KPH29_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBG1466511.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
     gene            43197..44105
                     /locus_tag="KPH29_000885"
     CDS             43197..44105
                     /locus_tag="KPH29_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBG1466512.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGVNIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
     gene            44110..46479
                     /locus_tag="KPH29_000886"
     CDS             44110..46479
                     /locus_tag="KPH29_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBG1466513.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKKDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFQSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRLTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPKDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            46804..47607
                     /gene="proC"
                     /locus_tag="KPH29_000887"
     CDS             46804..47607
                     /gene="proC"
                     /locus_tag="KPH29_000887"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBG1466514.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAEDITVSDLSQVALDKV
                     KEEFGINTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
BASE COUNT        19522 a         5119 c         8545 g        14669 t
ORIGIN      
        1 gtgaaaggtt ttaacataga caaaatagat tttgtttata gtgtaaagcc aatagatgtg
       61 tttttaaaga catttcctac attattatgg tatacaacat ttccactaat gatattaaca
      121 ttaacaaaaa atagatttac aacaatagca atattaatta catatacatt tgctgatata
      181 ttttttattt tacatatgta cccatttgtt agtatgatta atgtaaatgg attttatatt
      241 caaaagatgt tttatatgcc taataatata gatgttagat ttaatgaatt aaagaattat
      301 ttctttataa atagaggttc tctagtagtt atttctataa taatagtttt ttatatagca
      361 agaaaatcaa cggttttaaa aaagagtaaa tagtttaata tattttttat aatgttatat
      421 ttatatagaa tagtgacttt aaaaaattta aagccactat tttttaatat gttttatcta
      481 tataactatt atttttctag gttttttaat atttcgtaat agtgtgttgt ggctatttca
      541 cttgactcgc cttttttaca aaaagataat ttagatagtt ttggaactac acgttcaacg
      601 acaattttat tacctatcat tttagattca agtatcttca atgcttctaa aaatctctta
      661 tccttttttg cacgattata aaatgataaa acgtagacat aaaggaatag gttataatta
      721 cggaaaggat attcaacttg cataaataat gtgccaattc catagtgaca agggccgagt
      781 ggttttttaa tagtccaatg gtcaagtaga aaatctacag ctttgtcaag ttttgattct
      841 ttatttagat aattactaaa acggaatgca tttaatatat taagagttgg tagtggattt
      901 gagtattcag tttctggacc tcgaccaaaa ctaaatttat tgcaacgcca cccaccatca
      961 gtatactgtg tatctaaaaa atgttgaaac gttttttgca ttctaatgtc agatgcatat
     1021 cccatatgac agagtacgtt agcagcattt gcagtatgac aagggtaaat acctccattt
     1081 ggatatagtt tgaaacgtcc atcttctcgc catgtgctaa atatcaagtc agcacattct
     1141 ttaagtaaag tatcatcagg gttcacacct aactctaata gataaagtac actatcaaga
     1201 gtagagaaag gagaaccttt tattaaacgc ttatcaggtg tagtccaata gtctgcacca
     1261 ttatcatacc tgtgtgataa tattatttca atatctgata aagattgatt ttctacattc
     1321 attttttacc ccatttcata ttatattcta ttaaatctat caaaaacttt attataatat
     1381 tatttatatt tataattgat ttattttatt taaaattgca tttatagctg tatcaggaac
     1441 aactgctaaa tcttctgcat tgaagtaata aactatatta gaatcaaata atatatttga
     1501 atttggagga aattcatcat ctccaaacca aagtataaat atcatagatg tattacccat
     1561 atatgtaaac ctataagcta aatctcccat gtttactttt tcagctccaa ttttctccat
     1621 aactaactta tatttgtcta tttgagatgt aaaaacttct gaaagcctat atagagtcct
     1681 gtttaaaaaa tttggataat aaacattgcc tcctggtatt tctttaaaag ttacatattt
     1741 attagttcta gggatacctt tagcattgat taaatatcta actatcaaga ttttaagctc
     1801 aaagttattg caaaggctac cattactatt ccaaacttct ccacttggat agtctataat
     1861 atattgtcta tcaaaaaaat tgagtgtgaa tctatttaaa tcattattga aaggtatatt
     1921 acttaaatta ctaattgtat gagggtctaa ttctttgagt ttttttcgaa catagtcaaa
     1981 tggaacatta taattatttt ttgttatatt ttcatccata attttttcct ccaatttaaa
     2041 caattaaaag tgtatttata aaaagttttc ttattattaa ttttattata tattcaaaat
     2101 attacaataa taaattgtta aataaatttt tatatttaag taaatgcgtg taaaatgaat
     2161 ataaagtttt tgaaatataa agtttttgta tctaaaatat tttatttgta aaatagtaat
     2221 taattattgt aagctataat atgagagtat tatggttaat gtgaatttta aagatattat
     2281 taaagatata aattagaata ctattaaacg gataaatgga ggagttatta tgtctaagat
     2341 attagtagta gatgatgagt tagatatgtt aaagttgatt gaaaatgtgt tgaaaagaga
     2401 tggacatgaa gtaaaggtaa ttagtgatgt agaaaaggct atgaacatgg attttaatta
     2461 ttttaatcta ataatattag atgttatgat gcctaagatg gatggttttg aagtatgtaa
     2521 aactataaga aataaggttg attgtccaat actgtttttg acagcaaaaa atatggaaag
     2581 tgatattatg tacggtcttg gcataggtgc agatgactat ataaccaaac cttttggtgt
     2641 aggagaatta agggctagga taactgctca tttaaggcga gaaagtagag aaaaaaagaa
     2701 tttattaact atctcaaatg ttcaatttaa ctttttaggc aaagaagttt caattgatgg
     2761 taaaaaaatt aattttacta aaagtgaata tctaatatgt gaatttctag caaaaaataa
     2821 gggacaagta ttttcaaaag aaagaatata tgagtcggta tatggttttg atggagaaag
     2881 tgatataagt actatcacag agcatataaa aaatatacga agcaaattaa aacaatatga
     2941 tatttataca atagaaactg tatgggggat agggtataaa tgggtgtaat aagtataagt
     3001 gttgcttagt gaaaatatta tatatatcta caaaatgttt gtaaaatatt aatctatata
     3061 ttaaaattta atttatagat taatattttt tgtaaatgac aaaattgtaa ggtattttgt
     3121 tataaaatat taagattcat agtgtatact aataatatag tatttagtat aaatcttgag
     3181 caaaggatgg ttatttctaa tggatagact aaagaatatt agaaagagtt tactacagta
     3241 tacattttta tttttgttaa taggtataac aatttaccta gtgtttaaaa cattggatat
     3301 taaaatgcta tcaaatgtta tggttatggt agataagaag tttttattca taggtgcttt
     3361 ggcaataatg tgtcacataa tgcttgaagg agtagtaatg aagataatta tagaaagcac
     3421 tcacaaagtt aatattagat ttattggttt taagctagcc attatgggat tttattataa
     3481 tttaataact ccttttgctt caggaagcca accagtacag atatatgtac ttaaaaaatg
     3541 taaaatgcca cttagtaagg caagtgcagt tgttaccaat aagtctatta ttttccaaat
     3601 agtggttact ttttattgta caatacttgt attaatgaat tttgctatgt tagaaaaaca
     3661 aatgaaaatg attatgcctc tagtgtttct agggatagcc attaattcat ttgtcttaat
     3721 tatgataata ttagttattt taaaccctaa aaaaataaaa tattttatga gaattactat
     3781 aaagcattta tcaaaaatta agtttttaaa atttttagat aataagattg aatccataga
     3841 aagttttata gatgattata gtaaagcaat aaattttttt gcaaagaata aaaaggtatt
     3901 aatttcaact ataatagtaa cttttattca attaagtgct tactttagtg tttcattttg
     3961 gatatataaa gcatttaatt tacaaggtta tagctatatt tatatactga cattacaagc
     4021 atttttatat atggcaatat ctccaatacc aacacctggg aatgtaggag ctaatgaatt
     4081 agcctttttc actatattca aatcagtatt ccctcaacct ttgatgggat atgcagtatt
     4141 tttatatggt ggatttatgt actatttaat tctgattgga agtggaatat ttactgtaat
     4201 tactcactat agaatgaaaa atagaattgg cagaaatatt gttttaagtg aaaactaaat
     4261 aagtttaaag aatatatatt gagtatatta aaaaatatgt cataagaact agaatatttt
     4321 aattaaatgg ttgaaatata aagattatta tgacatattt tttttgaaat ttaagaggaa
     4381 ttgtgagaaa attgttgaat ttaatagata gtattgcaaa atatttatga ttttgcatat
     4441 tataaaaaca ctcattatct ttaagataaa ttttacttat caggggataa atctaagggg
     4501 ggaattgtat ggagaaaaaa attttaacag tttgtccata ctgtggagct ggatgccaat
     4561 tatatctagt agtaaaagat aataagatat taagagctga accggctaat ggtagaacaa
     4621 atcaagaaag tttgtgttta aaaggtcgtt atggatggga ttttctaaat gaccctaaaa
     4681 ttctaacacc taggctaaaa aagccaatga taagaaaaca aggtgtacta gaagaggttg
     4741 aatgggatga ggctataaac tatacagctt caagactaaa tgaaattaaa gaaaaatatg
     4801 gacctgattc tattatggga acaggttctg caagaggacc tggtaatgaa gcaaactata
     4861 taatgcaaaa atttatgaga gctacaatag gaactaataa tgttgaccat tgtgctcgtg
     4921 tatgacatgc accatctgta gccggtctgg cttactcatt aggtagtggt gcaatgtcta
     4981 attctatacc agagatagaa aatgcagatg ttttatttat atttggatat aatggagcag
     5041 attcacatcc aatagtggca aatagaatag taaaagctaa aaaaaatggt gctaaattaa
     5101 ttgttactga ccctagagta actgaatctg caagaattgc tgatatacat ctacctataa
     5161 aaggtggaac aaacatggtt ttagtaaatg cttttggaaa tgtattaata gaagaaggtc
     5221 tttacaataa agaatttgtt caaaatcata cacaaggatt tgatgagtat aaagaaattg
     5281 ttaaaccata tacagctaaa tatgctgaaa agattacagg aatcccagaa gagcttataa
     5341 gaaaagctat gagagaatat gcaaaaggga aaaaagctat gatactttat ggaatgggtg
     5401 tctgtcaatt tggtcaagct gttgatgtag ttaagggact tgcatcaatt gctctactta
     5461 caggtaattt tggtagagaa agcgttggaa taggacctgt acgtggacaa aataatgttc
     5521 aaggagcttg tgatatggga gctcttccta atgtatatcc tggctatcaa aatgtaactg
     5581 atgataagat aagagaaaag tttgaaaaaa cttggggggt aaaattatct cctaataatg
     5641 gttatagttt aactcaagtt ccagacttag tactaaaaga gaaaaagctt aaagcttatt
     5701 atatatttgg agaagaccca gtgcaaagtg acccagatgc atcagaagtt agagaagcat
     5761 tagatgaatt ggaatttgtc attgttcaag atatatttat gaataagaca gcacttcacg
     5821 ctgatgttat acttccagct acttcttggg gagaacatga gggagtatat acatgtgcag
     5881 atagaggatt ccaattgatg agaaaagcta tagagccaca aggagatgta aaaccagact
     5941 ggcaaattat aagcgaaatt tctacagcaa tgggatatcc tatgaattat aaaaatacta
     6001 aggaaatatg ggacgaactt agacaattat gtccaagttt cttaggtgct acttatgaaa
     6061 agatagaaac tcaaggatgc gttcaatggc cttgtaagag tgaaagtatg gaagataaag
     6121 gaactatgta tctgtatgaa ggtcaaaagt tttctactcc taatggtaaa ggtaatttgt
     6181 tcgctgctga atggagaccc cctatggaag ttgaagatga tgaataccca tttagtttat
     6241 gtactgtaag agaagttgga cactattcag taagaacaat gactggtaat tgtagaacac
     6301 ttagttcatt agaagatgaa ccaggacgtg ttcaaataaa ttctaatgat gctgaaaaat
     6361 taggaattga agatgatgaa ttagttagaa ttagttcaag aagaggaagt gtaataacaa
     6421 gagcaacagt tactgataga gttaaggaag gtgcaacata tatgacttat cagtggtggg
     6481 ttggagcttg caatgaactt acaatagcaa atttagaccc tatatctaaa acaccagagt
     6541 ataaatattg tgctgttaaa cttgaaaaac ttgaagacca agaattagca gaaaaatgtg
     6601 taagagaaga gtatcaaagt ttgaaagaca aaatgacagc tacaaatatc taatgattta
     6661 ttagatttaa gatttataaa aggcagaaag gctaatattt tatgaacaat atatactcaa
     6721 aaactaaatt acaagaaaat agtaaggata aagtttttga aaatagttat agtctagtgg
     6781 actacaaata tttaaattct tttaatgtag aagtaagcaa aataaatgaa catgaagtat
     6841 caatagataa tgaagaaata attgcagaat atccacttag ttttatttta aatgataaat
     6901 atgtaaatac atttttatgt accccatata atttagagga acttatagta ggattcttga
     6961 aaacaaaagg atacatagaa aacaaaaaaa gcttattaaa atttgagata aatgagagtg
     7021 aacgttttgc aaaagtaact attcaagaga aacaaaaaaa tcaagatgag caaattatat
     7081 ttttaaattc actagactat ataacatgtg cttctgtaaa gagcaatata aaaataaatt
     7141 atgatacgat ttacagtata atggatagaa atttaaattc atcagaatta tttaaaaata
     7201 ctggtggggt acatagcgta tctgtattta attttgataa aaaaatagtc acttgtgaag
     7261 atgtagcaag gcataatgca atggataagg ctattggatt ttgtgtttta aatgacataa
     7321 cattaaaaga taaaattgtg gctgtaagtg gaagaatatc attggaaatg atattaaaag
     7381 cagctatgac tcaaattcct attgtagtat caaaatcagc accgactaat ttatcaatag
     7441 agctttctaa aaaattaaat ataacgctta ttggatttgt aagaggaaga cggatgaata
     7501 tttatgcaaa tccacagaga gttatcaaaa agtgagaata agtatatgtg tactaaaaaa
     7561 gaagaacaaa aatgttcaaa gtgtgaaatc tgtggagtat taattgacga tactgataag
     7621 gtgtataata acacatacaa tttgtgcaaa aaatgtaaac gaaatataag tactgagaaa
     7681 atagtgaaat atataaatat atatgatgaa agtaagtaga catatgatga aagtaactag
     7741 agggttggaa atatacaaat tgaaaggtgg gataatatga aaaataaaat aggctctttt
     7801 gtaatagcgg acccaaacaa atgtacaggt tgtagagctt gtgaagtagc ttgttttaca
     7861 atgcataatc agcataataa tgtgggttat acagttggaa cagttgatat acctgttata
     7921 cctagacttt atttagtaaa aggtgacaat ttctgtatgc caatacagtg tagacattgt
     7981 gaagatgctc cttgtttaaa ctcatgtcca caaaaagcta tagtcaaaga aaacaatatt
     8041 atgtctgtaa atgaagaaaa gtgtatagga tgtaaaactt gtctgctagc atgtcctttt
     8101 ggagctatag acttacttcc acaatatgaa gatggtaaag aagtagaaca agtaatttta
     8161 gatgaaaata agaagatagc ttataaatgt gacttatgta aagacaatga aaaaatagct
     8221 tgtataagtg catgtcctca ggaagcatta aaacttgtta ctccaataga tgacaaaaaa
     8281 gctaagaata gacaagctgc acttagttta ttgttaacaa ataaataaag gatacagggg
     8341 taggtgacgt ttatgataat aaatatagat aaggatttat gtactggatg tagagaatgt
     8401 tcaaaggtct gtccagtaaa tgctattgaa ggtgaagagg gaaagccaca agagataaat
     8461 ttagatagat gtgttatgtg cggtcaatgt gttcaaactt gtaaatctta tgcctcagtt
     8521 atagatgaag gatttgagtt tttacaagag aaaaaacaag aaagagagat accagagtct
     8581 ataaatgaac ctatatttgc agcatacaat gtatgtgata tagataaggt aaagaaagct
     8641 ttaagtgacc caaatgtatt cactatggtc caatgtgcac cagcagttag agttgcttta
     8701 ggagaagatt tcggatatta tttaggatac ttaggtgctg gaaaaatggc agcagcactt
     8761 agagctttaa attttgatag agtctacgat actaattttg cagcagattt aactataatg
     8821 gaagaaggta gtgaattaat taaaagagtg acagaaggtg gaactcttcc aatgtttaca
     8881 tcttgttgtc cagcttgggt aaaattcatg gaaaaaaatt atcctaaatt aactaatcat
     8941 ttatcttctt gtaaatcacc tcagcagatg ggtggtgcaa tatttaaaac ttatggtgca
     9001 gagataaata atgtagatgc atcaaagata tttagtgttt ctataatgcc atgtacatgt
     9061 aaaaaatatg aatgtgatag agaagaaatg gattctagtg gatatagaga tgtagatgta
     9121 gtattaacta ctagagaact agcttattta ataaaagaca tgggaattga ttttaaaaat
     9181 ttaagagaag aaaaatttga tagtccttta ggtagttata gtggagctgg aactatcttt
     9241 ggtgttactg gtggggttat ggaagctgct ataagaacag gatatgagct tataactgga
     9301 gaaagtattc ctgatgtaga agtaaaacaa gtaagaggag aaaatggttt tagaaagtca
     9361 actattaaaa taggagactt agacttaaga gttggagtag taagtggatt aaaaaatgta
     9421 ataccagtgc ttgaagactt ggaaaaagga aaattagatg tagattttat tgaagttatg
     9481 acttgtcctg ttgggtgtgt aagtggtgga ggtcaaccaa aaattttact agaagaatat
     9541 aaggaattgg cttatgaaaa tagaacaaaa gcaacatacg tgcatgatga acatttagca
     9601 attagaaaat ctcatgagaa tcctgatatt aaaaaactgt atgatgaata tctggtagaa
     9661 ccattaggag aaaaatctca tcatctatta cacactacat attgtatagg aaaggaagtg
     9721 gctaagtgat gaactatttt gttatagcag accctgataa atgcatagga tgtagaactt
     9781 gtatgatagg atgtgtagtt gcacattcag atgaagatat tttttaccaa aatccagatg
     9841 aaataaattt taatccaaaa ctttctgtca taaagacaaa agaagtaagt gctccaatac
     9901 agtgtagaca ttgtgaagat gctccttgtg caaaggcatg tccaaatggt ggtatagtta
     9961 gggttgggaa cacaataaaa ataaatgaag aaaattgtat aggatgtaag acttgtatgt
    10021 tagcatgtcc tataggtgca atagacatag ttacattaaa agatgtagat gaaggaaagc
    10081 tttgctttag agaaagaatg acagcaaata agtgtgattt ttgtatagaa tcgccagaag
    10141 ggcctgcatg tgtaaatgtt tgtccaacta aggcatttac tatagttaag gatgaggata
    10201 tagatagtaa agttaaaaat aagagaaaat tagctatatt gtaatataaa aaagatggag
    10261 ttttatttat gattaaaagc tccatctttt ttattataat atttatatat ctaaaaatca
    10321 attattgcat tagtttatct aatctttctt tattcaattt tttcattcct atagaagtta
    10381 taataagtaa agtataggca attataactg ataaaattat aatagaccaa gaaaatctgt
    10441 atccaaaatt atcaactaac aatccaaata atgttgaacc taatgcaaat cccactgcaa
    10501 aaattaattg tacgattcca tatatgctac caaactcctt ttgaccaaag aaagagccag
    10561 ttaaataagc aggaccaatc atataagcaa atacagaaag acctttcaaa atggcaaata
    10621 caaatgcaaa agtagaagat ttatctacca atagtaatga taaagttgct ataagaacca
    10681 atacaaaaga tataataagt ggctttgttt gaccgaattt atcaaacaat aatcctccaa
    10741 ataaattacc aatgagagca aataaagcaa atgtggagcc tactgttcct attagaatag
    10801 gatttagata atttctaaga taagttggat attgtacgga taatgatgaa acatatatac
    10861 caacaaatat aaatccaata gtcataatcc aaaaatattt aattttttta gcttctttta
    10921 atgtataacc ccatactgaa ggagaaattg tatcagatgc tttctctttt ttacctttag
    10981 attttacctt ttcattaata tctttaggca tcctgagtat gaataatatt acaggtatac
    11041 caactattaa tgctataatt ccaaatatta gataagaatg actgtatcct tgagttgcta
    11101 ataagcgaac tgcaagtggt tgtagaaata tactaccaat agaaccacca gcaaaagcaa
    11161 agcccaaagc ttttcctttg ctttcttcat caaaccaagc acttattaaa acaggtattc
    11221 ctatagcaga tataattgta gcgccaattt gaacaataca tgatacagtg taaaattgcc
    11281 atagttcagt acatatagaa aaagtagcaa aagcaatact agaaattata caacctaata
    11341 aatatagtat cttaatattg actttagaaa ataatacacc tataaaaggt gatgcaattg
    11401 ctgaggcaat agtaccaata gtgaatatta gagaaaaaga agctaaacta aagccttctc
    11461 cttctacaac ataacttata aattgaggct gtatacttgc tacaatacta aatggaacag
    11521 cttgtattaa catacaacca agtactataa cccaagcatt tgaaaagttt ttaatgctaa
    11581 atttattgtt tagtaatgta ttcatggtat ctctccttta ggttatgatt tgtagatatt
    11641 aatctaatta ttctaatttt atatattagt tttggatagc ctaactattt aaatgtaatt
    11701 tgggtattaa tataaaaatt aactataaaa attgattatt ttaaatgtat ggggtaaatt
    11761 aaagtatata aataaatgaa aggatggata aaatgtcatt tgaaaaaata aataataata
    11821 acctagataa aatacacaat aggaagtgta ttatgcttgt aaactttaat aaaaaagaat
    11881 tatcattaat taaaaacgta agtaactttg ctggaataag agaccatata atcttaagtg
    11941 aaaaaaacgg agagaatata ataagagaca ttttagatgg aaatatatca agtgaatgtg
    12001 aaaatgggat taagagtaag tctgtaatat ttaataacac tccaagcaca caagtaaatg
    12061 catttttaga aggtctaaaa aaagtgagaa taaatagacc ttttacagct atggtaacag
    12121 agacatctat tgattggaca ttgaataatt taatagaaaa tttagttcaa gaacgtaatg
    12181 ctatgtcttt aggaaaaaca attgaacata agtaaattta agatatctta tagtaatttt
    12241 aactaatgta cattgagtat catattatat tttagaatat aaaattttaa atgtgggtag
    12301 atagaggaga tttgagatga aagttttatt tacaataaat tatggaaaag aaaaatttga
    12361 taaaataaga gaattaggct atgatgtcat acattactct gaagatagta ttgaaaataa
    12421 tgaggatgta aatacagcag atgtactagt tacatacaat ccatttgaga gattagatat
    12481 ttctaagatg aaaaatttac aatatataca gacaactagt gtaggtatag accaacttcc
    12541 aaaagaagag gtattgaaga gaaatattaa gatagctaat aatagaggcg gatatagtgt
    12601 acctattggt gaaagtattg tattgtatat tttagaaata tataaaaata gtgcaaaatt
    12661 ttttaaacaa caacaaaata aagaatggaa gatggatttt tctgtatcag aattgtatgg
    12721 acaaagagtt ggatttttag gaacaggtac tatatcttca gaagcagcaa aacgattaaa
    12781 agcttttggt gtagaaatat ggggtgtcaa tactgatgga agtagtaaaa aatactttga
    12841 taagtgtttt gctactgaag atatgaatat tgtatttgaa gaatgtgata tagtagttgt
    12901 agcaatgcct tctacaaaaa gtacagatgg aatagtaaac aaagatatgt ttaaacttat
    12961 gaaagatggt tctgtattta taaatgtagg tagaggaaat acagttaatc agaaagattt
    13021 agaagaatgt gtagagaaat ttagaggggt agcactagac gtatttgaaa gtgaaccttt
    13081 aaacaaggac aataagttgt gggagtgcga aaatgtcata gtaactcctc ataattcttg
    13141 ggtgtcagat aaaaacaaag agagaacatt taatatggtt tataataatt tgaaacacta
    13201 tataatggag aataaacctg tagataatgt tgtagacata tcaagaggct attaaaaata
    13261 aaaaattttg ataaaaataa aaactcttat tagtcgtatt aaagatacta ataagagttt
    13321 tttttgtaat aaatttaaag tcaaaaaagt gatatactaa aaaatatata aataggagat
    13381 aattaattat agggaaattt caaaataatt taattttaaa aatatgaaga ggtgcaaaaa
    13441 gtgagcgtaa aagatgaaat taataaactt atagagacaa taaactacca taatgaaagg
    13501 tattataatc aagatagccc agaaatatca gattatgaat acgataagtt aatgaaggag
    13561 ttaatttctt tagaagaaga acatccagaa ctaaagagaa ttgattctcc aacaaatagg
    13621 gtaggaggta agccattaga taaattcaat caaattgtac ataaaactcc tatgttgagt
    13681 ttatcaaatg ctttttcaga gcaagatttg agagattttg ataagagagt tcaagaatat
    13741 gtgggagaaa atgttgaata tgttgttgaa tttaaaatag atggtctttc tgtaggtcta
    13801 acttataaca atggagagtt tgaaaaagga gcaactagag gagatggagt tgtaggagaa
    13861 gatatttctc aaaacttaat gacagtaaaa agcatacctc ttaagataaa tgataaaaaa
    13921 gaactaatag ttagaggaga agtatatata tcaaaagata attttagaaa agtaaatgaa
    13981 caacaagaag aacaagaaca gccattattt gctaatccaa ggaatcttgc agctggttca
    14041 ttgagacaat tagacccaaa actaacagca aagagaccat tagatatatt tgtatttaat
    14101 atggaaaata tagaagaata tgatttagaa agtcacagtg aatcactaga gtacttagaa
    14161 aaactaggat tttcagtaag tcagagttat aaagtttgta aaagtataga tgaagttatt
    14221 gagcatattg aatactggac agataacagg ggtaatttag actacgaaat agatggtatg
    14281 gtaataaaag taaacaatat aaaacaaaga gacgaaatgg gatatacagc caaaagtcct
    14341 agatgggcaa ttgcatataa attcccagca gaaaagaaga aaactaaaat agtagatata
    14401 atagtagaag ttgggagaac tggtacaata acaccttcag caattcttga accagtcaga
    14461 cttgcaggta ctactgtaag tagagctaca cttcacaatg aagattatat aagagaaaaa
    14521 gatataaaaa taaatgatac agtattagtt caaaaagcag gggacataat acctcaagtt
    14581 ttagaagtta taaaagagga acgtactgga gatgaaatag attttaaaat gccagaaaag
    14641 tgtccagttt gttcagagcc aacaataaga ttagaaggag aggctgctgt aaaatgtatc
    14701 aatatgtctt gcccagccca aataagaagg ggaattatac actttgtgtc aagggatgca
    14761 atgaatatag atggattggg agaatctata attacactgc tattaaatga aaaaattatt
    14821 caagatgtat ctgatttata ttatataaaa aaggaagatg tagtcgacct agagagaatg
    14881 ggcgaaaaat ctgctgacaa tttaataaat tcaattgaaa aatcaaaaga aaatgattta
    14941 tggagactta taaatggatt aggtattaaa tttataggag ttaaagcagc taagatactt
    15001 gcatataact ttaaggattt agatgaagta atttctgcaa ctagtgagca actagaagaa
    15061 ttagaggaat ttggaagtat aatggcaaac agcgttgtcg agttttttaa ggaagacaaa
    15121 aatatgaacg ttataaacaa gttaaaaaat gtaggagtaa atacaaaatc attggataat
    15181 acaggtgaaa aagtagaaaa aatatttgaa gggatgaaaa tcgtattgac tggtacactt
    15241 cctacattaa aaagaaatga tgcgaaagat atgatagaaa aaagaggtgg aaaagctaca
    15301 tctagtgtta gtaaatcgac aacatttgtt ttggcaggtg aagaagctgg ttctaaactt
    15361 actaaagcaa atgatttagg aattaaagtt atagatgaag agagattttt agaattatta
    15421 aagttatcat cgaaagatga agtaaaagct gtattagaga gttaaaaagt cgcttaattt
    15481 agtagaaaac tactataatt aatgatagaa tagttcagta agctaataag attacgaaaa
    15541 atgacaataa ttaatatttg acaaaattaa aaattataaa aataaattta taaaaaataa
    15601 aaaatatgag aaaggatata aaaaatgacc agaagttatg atagaagtaa cgaacagata
    15661 agacctgtaa agataactag agattataca aagtatgcag aaggttctgt tcttatagaa
    15721 atgggagaga caaaggttat atgcactgca tcagtggaag aaaaagttcc tcctttttta
    15781 agaaatagtg gtactggatg gataaatgct gaatattcta tgctaccaag agcaacacaa
    15841 caaagaaaga taagagatgc atctagggga aaaattgatg gaagaacaca agaaatacag
    15901 aggttaatag gaagagcaat aaggtctgtt gtagacttga ataaattagg agaaagaact
    15961 atttgggtag attgtgatgt gattcaggca gatggtggaa ctagaactgc atctataaca
    16021 ggggcatttg tagcagttgc agaagcaata tataaattat ataaagcaaa gacaataaaa
    16081 gagatgccaa taacaaattt tgtatctgca attagtgttg gtattgtaaa tggaatacac
    16141 atgctagatt tatgttatga agaagattct aaagcacagg tagatatgaa tataataatg
    16201 actgataagt gtgagtttgt agaagtacag gggacaggag aagagaaacc tttttcaaga
    16261 gcagatttaa atttattgtt agagttaggc gaaaaaggga ataaagaatt aataaggctt
    16321 caaagagaag ctttaggtga aatagcagat gagatattgg gtatggatta tggtaatgaa
    16381 gtagtgattg ctactaataa tgctcataag ctagaagaaa taggtgaaat cttaaaagat
    16441 tttgaatata aagtgtactc tttaaaagat gttgatttag caggtataga gatagtagaa
    16501 gatggaaaaa catttgagca taatgcgttg ataaaagcta gagcaatagc aaaaaagact
    16561 aaacttatag caatatcaga tgattctggc ttagaagtag atgctttagg caaaaaacct
    16621 ggtgtttatt cagctagata tgcaggagaa catgcaacag atgaagaaaa tagaaaaaaa
    16681 ctgttaaaag ctatgcaaaa tgtaccaatg agcaaaagaa atgcaagatt cgtctctgca
    16741 atagcagtag tttttcctga tggaaaagaa tttgtagtaa gaggaatttg tgaaggaatg
    16801 ataggatttg aagaaaaagg taaaaatggt tttggatatg atagcttatt tatagtaaaa
    16861 ggatatgata agacttttgg tgaaatacct agtgttataa aaaattcaat cagtcataga
    16921 gcaaacgcac taaaacttat gaaacaagag tttataaaaa gggtagtgaa gtaatgaaaa
    16981 taggcatagt aagcgacaca catatgatga ttaaaaatat ggaaaaaaca ataccatatt
    17041 tgaaagaatg tgacctaata attcatgcag gagataactt tactgattca agatatattc
    17101 atagcatgac taatgtaggt attattgcag ttaaaggtaa ttgtgacttt gatgctgttg
    17161 aggaagaagt tgtctttgaa gtggcaaata aaaccatatt cttatgccat ggagataagt
    17221 atggtgtaaa atatggaact aatatgttag aaaaaaaggc aactgaagtt gatgctgata
    17281 tagttatatt tggccatacc cataccccat ttagagaaat taaagatggt gtactttaca
    17341 taaatcctgg aagcacttca cttccaagag gagtatcgta taaaagtttt gtcattatgg
    17401 atatagaaga agatgacata aaaattgaag aaatacgcat ataaaggtgt atttatctaa
    17461 aaaaatggca aaaaacgctc aaaaaattaa attttatcaa ataagtacaa cgatattaat
    17521 ggaacacttg taaataaaaa aacttatgtg atataattaa gtggttgaat tataaataga
    17581 atgaattata ataccgttat cgggtattac ataaatatca ctaggaggat tagaaggatg
    17641 aaagcagaac taattaaaaa agagggtaat aaagttactt taaaaataac agtagataac
    17701 aataaatttg aagcagcagt aaataaggct tataataaaa gcagaaataa atacaatata
    17761 cctggattta gaaaaggtaa agctccaaga attgttatag agactcaata tggaaaaggt
    17821 atattctaca atgatgctat agaaatatta ttcccagaag tttatccaga agctatcaag
    17881 gaattagaca tagacccaat agacaatcca gatttagata tagaagaaat aagtaaagat
    17941 aatgggttgg ttatggttct taatgtagaa gttaaaccag agtttgaatt aggaaactac
    18001 aagggtatag aaatagctaa ggttgagaat actgtaagtg acgaaaatgt tgatgctaaa
    18061 ctacaagaaa tggttgaaaa aaatgccaga ttagtaagtg tagaagacaa agctttagaa
    18121 gatggagata cagctataat agacttcgaa ggttttgaaa atggtgtagc ttttgatggt
    18181 ggtaaaggtg aaaattataa cttagttata ggttcaaaca catttatacc aggatttgaa
    18241 gaacaattag taggtaaaaa agctggtgaa gaagtagaag ttaatgttac tttcccagaa
    18301 gaatatcact cacaagattt agctggaaaa ccagttgtat ttaatgtaaa aataaatgat
    18361 gtaaaagtaa aagaattatc tgcacttgat gatgaatttg ctaaagatac tagtgaattt
    18421 gattctttag atgaattaaa agcagatgta agagctaagt tagaagaaga agctaaaaat
    18481 agagcagatg cagaaactag aaattcagta gtagaaaaag ttgctgaaaa tacagaaata
    18541 gaaataccag aagttatgat acaacatcaa atagataata tgttaaatga acttaactac
    18601 caattacaat atcaaggatt tggattacaa caattactag agatgactgg tagaactatg
    18661 gaagaattaa gagaagagag aaaagaagat gctaaaaaat tagttaagtc ttctttagta
    18721 ttagaagcaa taactaaagc tgaaggaatt gaagcaacag aagaagaatt taaggctgag
    18781 ttagaaaaaa tggcttctgc ttacaatatg gaagtagaaa aaatagaagc ttctttaaga
    18841 gatgctgaca aagaagatat aaaaggtcaa ataaaaataa gaaaaacaat agatttatta
    18901 gttgataatg caacaatagc ttaataaaat aaacgctaac taatatagtt gacgttatga
    18961 aatgtttagt atataatgat gtgcaaatat aatttagtta aatgcgtata ataatttaca
    19021 gtagcttaaa aaagctactg taaattatat agaataaagg gggtatgaaa ccatggcatt
    19081 agtacctgta gttgttgaac aaacaggaag aggagaaaga tcttatgata ttttttctag
    19141 actattaaaa gacagaataa tcttcttagg agatcaagtt aacgatgcca cagcagggct
    19201 tatagtagca cagctattat ttttagaagc tgaagaccca gacaaagata tacatttata
    19261 tataaattct caaggaggaa gtataacttc tggaatggct atatatgata ctatgcaata
    19321 catcaaacca gatgtaagta ctatatgtat aggtatggct gcttctatgg gagcattctt
    19381 gttggcagca ggagcaaaag gaaaaagact agcactacca aatagtgaaa taatgataca
    19441 tcaaccatta ggtggagctc aaggtcaagc aacagatata gaaattcatg caaagagaat
    19501 tttaaaaata aaagaaactt taaatgagat attatctgaa agaactggtc agccattaga
    19561 aaaaattaaa atggatactg aacgtgataa cttcatgagt gcactagaag ccaaagaata
    19621 tggtttaata gatgaagtat ttacaaagag accgtagata gagagaggtg ttaatatgtc
    19681 taaatatgaa gaaaaacgac aattaaagtg ttcattctgc ggtaaaaatc aagatcaagt
    19741 aagaagatta attgcaggtc caaatgtata tatttgtgac gagtgtgttg aactttgtga
    19801 cgaaattatt caggaagaaa tagaagatac tatagatgaa gatactacat ctttaccaaa
    19861 accaaaggaa atgatggaga ttctaaatga ttatgtaata ggtcaagaaa aagctaaaaa
    19921 agctttatca gttgcggttt ataatcatta taagagaata tacagtaaaa aatctagtag
    19981 taaggatata gaaattcaaa agagtaatat acttttatta ggacctacag gttctggaaa
    20041 gacattactt gctcaaactc tagcaagaac tctaaatgta ccttttgcaa tggcagatgc
    20101 tacatcattg actgaagctg gatatgttgg agaagacgtt gaaaatatac ttttaaaact
    20161 tattcaagct gctgattttg atatagaaaa agctgaaaga ggtataatat atatagatga
    20221 gatagataag attgcaagaa aatctgaaaa cccatctata actagagatg ttagtggtga
    20281 aggtgttcaa caagcacttc taaaaatatt agaaggtact gttgcaaatg ttccaccaca
    20341 aggaggaaga aaacatcccc atcaagaatt tttaaagata gatactacta atgtattatt
    20401 tatattaggt ggagcttttg atggattaga gaagataatt caaaaacgtg gtggagataa
    20461 aacactaggt tttggagcta aaatagaaag taaaaaagaa ttagatttag ggaagctgta
    20521 tgaaaaagta cttccagagg atttattaaa atatggtata ataccagagt ttataggaag
    20581 aataccagtt ttagctactt tagagttgtt ggatgaagat gcattaatgc agatattaca
    20641 ggaacctaaa aatgctctag taaaacagta taaaaagtta ttggagttag atgatgttga
    20701 gctagaattt gaagaaggtg ctcttcgtgc aatcgctaaa aaggctatag aaagaaatac
    20761 aggagctaga ggacttagaa gtatagttga aagcgtaatg atggaaacta tgtttgaagt
    20821 accatctaga gataatataa agaaggttat agtaacagag aaatctgtga acgaagactc
    20881 agtaaatcct atcatagtac ttaaggatca agaggaaagt gcataagatg atgaaaagga
    20941 gctttaaaag ctcctttttt tatgttaata tgctaaatat aaaataggta tcattatcat
    21001 aaaaatgtga gtaatatacc tagaatacca aagacgaata caattgcaat ggaaccaatt
    21061 tttcctttat ataaaagata aaatgatgct ataaatgcaa ttattccata taaatcccaa
    21121 ttaacttctt tggcagatga aaaagctgct gataatatga atcctagagt aattggtcta
    21181 agaactgtca ataagttttc taccttttta ttatttttaa atttagaaaa taactttgat
    21241 ataatagtta gacctattaa tgcaaaaatt gtaacaccaa tagttgcagc tagagaacca
    21301 aagaagccag cagtcttaaa tcctacaaat gtagcactat ttattgctac aggacctggt
    21361 gtagattgtg atatagcaag taaatccaaa aactcttgat tagttataaa gtgatattta
    21421 gatataaatt cttgctggat aaatggtagc atggcataac cgccaccaaa actaaatgcc
    21481 cctattttaa ggaatattaa gaagagtagt attatttctg acatagacta tatacacctc
    21541 ctccaattat taaataaata gggcttatat tgaatacacc aacagataaa actgctataa
    21601 ttaatagtag tatgttagtt ttagactttg gtagtttctt gaacatgctc gtaaaagaat
    21661 acaatataag agctattaca acaggcgcaa ctcctttaaa aaatccatct aaaattttag
    21721 atgatgaatt tttaaagtat agatacgata agactaagac tatacaaaat gatggtaaag
    21781 cagagcctaa ggtacatata agaactcctg gtataccata aagtttatat cctaataata
    21841 cagatatatt aactgccagt actccaggat aactttgagc tatggataaa taatcaataa
    21901 attcttcctg ctctaaaagt ttttgtttgt ttacaagttc atcttgtata agaggaacca
    21961 tagcgtatcc accaccaaat gtaaacgcac caatttttaa aaaggttaaa aacaatttca
    22021 acataataga aacctccaaa agataaataa catctaagta tactaatatt aatattatat
    22081 cataataaaa agataatata gtatgatagt attctaagaa tttatcatta atgttgaaaa
    22141 ttaacttgtt caaatatata atattaaaag caaagcaatt agactagaaa aggagtaatg
    22201 ttgatggaac aaaattatac taaaatagat catgaattac cattaattcc tctaagagga
    22261 ttagctatat ttccatatat gatattaaat tttgatatag gtagagagat atctcttaaa
    22321 gctttagacc aagctatgat ggatgaagaa cttatatttt taacttctca gaaagaagca
    22381 gaggtagatg agccaggaga agaggatttt tatcatgtgg gaacaatctg taaagttaag
    22441 caaatgataa aattaccagg agatacagtt cgtgttttgg ttgaaggtgt atcaaggggt
    22501 agagtaaaga aaatagaaca agaagatggg tatttcagag cagtaataga agaaatagta
    22561 tttaatagtg ataatttgga tagtgaaaca gaagttgaaa ttgaagcatt tgtaagaaat
    22621 gtatttgatg cctttgaaga gtatataaat attggaaata gggtatcacc agaaatatta
    22681 atatctctag ctgatataga agatgtagat agatttatag atacgatagc tgcaaacata
    22741 tatctaaaat caagccaaaa acaagaaata ttagaagaat ttgatattag aaaaagactt
    22801 gaacttattt attctatatt attagaggaa atagatatat taaaaataga aaagaaaata
    22861 actttaagag taaaaaaaca gatgaataag gttcaaaaag aatattatct tagagaacaa
    22921 ttaaaagcta tacagaaaga attaggtgaa gaagaagata taaattctga agcagatgag
    22981 tatagagaaa agcttaaaaa aataaaagca cctaagacaa ccaaagaaaa aatagaaaaa
    23041 gagatagata aattttcaaa aatatcttct atgtctccag atgtatctgt aagtagaaac
    23101 tatttagata ctatattctc attgccatgg aataaagaga caaaagataa attagatata
    23161 actaaggcaa aagatatatt agatgaagac cattatggat tagaaaaagt taaggagaga
    23221 atactagagt atctagctat aagaactctt gctaagtcac ttaaagggcc tataatatgt
    23281 ctagttggtc ctccaggaac aggaaagaca tctatagtaa aatcaatcgc cagagcatta
    23341 aatagaaaat ttgtaagaat atctttgggt ggagttagag atgaagcaga aataagaggt
    23401 catagaagaa cttatgtagg ttcaattcca ggaagaataa ttaatggggt aaaagaagcc
    23461 caaacaaaaa atcctgtatt cttatttgat gagatagata agatggcagc agattataaa
    23521 ggtgacccag cttcagcaat gttagaagta ttagacccag agcaaaataa ggactttgtg
    23581 gaccattact tagaaatacc ttttgattta tcaaaaatac tatttgttac aacagcaaat
    23641 agtctaggta atataccaag accattatta gatagaatgg aagtaataga agtatctggg
    23701 tatatagaag aagaaaaatt aaatatagca agaaaatacc tacttccaaa acagattaaa
    23761 gaacatgctt taaaagaaaa ctttataaaa atagatgatg aaactttaag aagtataatt
    23821 aaccattata caagagaagc tggtgtaaga acactagaaa gaactattgg taagatttgt
    23881 agaaaggttg ccaaaaaata cgttgaagac ccaactcttg aagaggttgt tataaataag
    23941 tctgacctag agacatatct tggaaaagac atgtttaagt atcaacttgc agaagttaat
    24001 cctcaaatag gattagtaaa tggtcttgca tggacagcag ttggtggagt tacacttgaa
    24061 gtagaagtaa acgtacttaa aggtaaagga gaaattgtat taactggaaa attaggagat
    24121 gttatgaagg aatctgcgaa gacaggtatc tcttatataa ggtcaatagt ggacaagttt
    24181 gatatagacc ctgaatttta taagacgaat gatattcata tacacattcc agaaggcgct
    24241 gttccaaaag atggaccatc agctggtata actatggctc ttgcagtaat ctcagcactt
    24301 actaaaagac ctgttcctgg aaatatagca atgacaggag agataacttt aagaggtaga
    24361 gtcttagcag taggaggggt taaagaaaag ttattagcag ctcatagagc tggtataaca
    24421 aaagtattaa ttcctaaaga atgtgaagct gatttagatg aaataccaga aaatgtaaaa
    24481 gaaaaaatgg agtttgtgct tgtagaacat atggatgaag tattagaaca agctttatta
    24541 aagagtggtg aaaataatga aaattagaag ttcagagata actatgagtg ctgtgaataa
    24601 atcacagtat ccagcagaag gaatcccaga gatagcattg gctggtaggt caaatgttgg
    24661 aaaatcatca ataataaata cattgcttaa cagaagaaac ttcgctagaa ccagtcaaac
    24721 accaggaaaa actagaacta taaattttta tctaataaat aatgaatttt attttgtaga
    24781 ccttccaggc tatggatatg caaagatagc taagtctgaa aaagaaaagt ggggaggtat
    24841 aatggaaaga tatctagaaa gtagacaaga gttatgttct atattcctac ttgtagatat
    24901 tagacatgag cctacagcag atgataagct tatgtatgaa tggataaagc actttggata
    24961 taactgtgta gttattgcaa ctaaggcaga taaaatatct agaggacaat atcaaaaaca
    25021 cataagtata ataagaaaaa aattgcaaat ggaaagtagt gaaaaagtaa ttcctgtttc
    25081 ttcactaaaa aagactggtg ttgaagaatt atgggaagag attgtaaatc aatacaatca
    25141 acatggatat gaaataacag tagattaaat ttaaaatgtt aaatattgac ataagattta
    25201 tttgaataat aaatgtaata atagttagtt ttaattatat taatattaaa gcaaaatata
    25261 ggatgcctta aaatagtttt aatgaacttt ttttggcatc cttattgttt ataaacaata
    25321 aatatcagta attttgctaa atactaataa attttagtaa ggtatgggaa aattagtata
    25381 aattagttca actaaaaaat tattgactta tgtagtcagc aataaatagc atttaagaaa
    25441 tagatttatc tgtttttctc ataaagaaaa cagttagtat aagccctata aaacatactg
    25501 atgctgcaat ccaataagac tgattgctag ctataacata ggctgatgtg tgacttttaa
    25561 acttttgatt gtatgtaaga ctttgtgcaa aaaaactact aaaaaatgct gttccaagag
    25621 caaatgataa attacgaata gttgcaagaa aactgcctcc gtacccctgt ttttgaggag
    25681 gaacatctgc taaaatacta ctattgtttg gtgaactaaa tgctcctagc ccaagtccaa
    25741 aaataactaa acatacaatt aaaagataca atggagaatc tgcttttaaa agtgccattc
    25801 caactagtga gatggtcata aatgaaaaag atgctgttag aatgtttttt gcaccgtgtt
    25861 tatcagaaag tgaacctgca ataggtgcta taagtaccat aataagtgga tgtacagtca
    25921 ttacataacc agccatcata ggagataaat tttttaattg ttctaaataa aatggaagta
    25981 gaaaagatac tgccatttga ggaaaataac aagctactcc aatgatattg cctacagtaa
    26041 agtttctatt tttaaataga ggtaggtcaa taaagctttg ttcaaatttt acttctctta
    26101 atgccaacaa taagaagatt ataagtgctg ttatacctaa atacaatctt gtattaccct
    26161 tctcattcat tgcaagtaat ataagcgtag tgcaagatgc gaaaagtaga agacctgaaa
    26221 tatcaaagct cttgttttgt tcatccaaaa caggtttagg cagaaattta atgccaagaa
    26281 agactgctat aataccaata ggtatattga taataaaaat agaaggccat ccaaaatgtt
    26341 ctaaaattat acctccaatt acaggaccac tcatatttcc tataccaact acagctccag
    26401 tgattcctat agcttttcct ttttcatttg ctggaaatgc attggaaact ataccaagcc
    26461 cagtagccat taaaatactt gctccaactg cctgaaagat tcttgaagaa attaatgttg
    26521 ataaattgtt tgacatacta cacagaaaag aacctattgt aaatataaaa aatcctaatg
    26581 cgtatagacg atttgaacct actttatcac caagttttcc gaaaaataac atagtagctg
    26641 ttacaacaag catataagct gttacaaccc actgaatttg tgacatgtta attttaaagt
    26701 cagcagcaat tgtaggattt gcgatattta gtatacttcc atcaagagta gacataaata
    26761 caccaataca gatgataatc aatactatcc atttttgttt catgataaca ctccaatctt
    26821 aatagatttt ataagtacga tataattgta aaataaacaa caaaaaaagc acctgtatat
    26881 taggtacctt gaatgtgtct aatttcatga ataaatacgg tagttgaagg atttgtatag
    26941 attactcatg taaaattaca taaaggtact catatactat tttcttctac catccagatt
    27001 ttactgtcgg ctttggaata tgaccaaatt atgccataag gctcgcgggc ttttaccgcc
    27061 gatagggaat ttcacccgtt tatgaagaaa aacagatgta ttttgtaata ttattttaac
    27121 ataaaattat aatggaaaca attggattta agtaatatcc agctgtatta caatacaaat
    27181 atttcaatat ggacacttaa attgtattta tagatagaat aaggtacagt atgagaatct
    27241 ataataatta gataaaatta atatgttatt agtattagat ggtaagtttt aaatttgtca
    27301 aaatcagaaa gctgtaataa aattcttata atatgaatat cttaattaag agatattgta
    27361 aattatgagg aggcgctaat atggaaggaa aggtaaagaa ggcattccca ggtggaaata
    27421 catcacaagg ctcatattct ttttttgact atataattcc tgaaaatgta aaaagagttt
    27481 tttgtttaaa gggtggacct ggagtaggaa aatcctcact tatgaaaaaa atttataaag
    27541 agtttttgca tagaggatat gatattgatt tatatcattg tccttctgac ccaagttcgt
    27601 tggatggttt agttataaag aaattaggcg tggtacttat ggatgcaact gccccacata
    27661 ctatggaccc aaagctacct ggagctttgg atgagataat taatttaggt gattactgga
    27721 atacagaaga attagaaaag aataaacaag aaatatcagt ttatgataaa gatattagta
    27781 attcatttaa aagagcattt aagtttttaa agtcagcaga accaattttt agagacatag
    27841 aagaaaaata ttctgattgt atggattatg gcaaagtaaa tttggtcaca gaagaattta
    27901 taaaaaggtt atttgatgga gttaaaagca caggaaatct aaaaagagaa aagcatttat
    27961 ttggttcagc tataactcct gtagggtgtt tagattatac agaaaacatt ttaaaagatg
    28021 taaagaaagt ctattattta gatggagaaa taggaactgg gaaaactaca ttattaaaca
    28081 aagtatataa gaaagctact gaaaaaggtt taaatgtaga agtttatcat tatccactta
    28141 ttccagagaa aattgaaact gtactattaa ctgatttaga tataggcata actataagta
    28201 cgacttttaa agaagaagat acaattgatt taaatcagtt tttaaataga gaaaaactac
    28261 ttaaatatga agaagatatt aaatttgatg aaaaagtttt agatgattta atttgttggg
    28321 cagtactaaa tcttaagaga gccaagtcta aacatgatat tatagaaagt tactatagtc
    28381 ctaatatgag atttgatgaa gtagggaaat taagagataa gttaattaaa agaattttaa
    28441 aatatgagga aaacttataa acgtatatgt ttaaaattaa tgttaataat aatataattt
    28501 ttagtaaatt ggtgtagctt tgaggctatg ccattttact tttttttgct aaaaaatgat
    28561 attaataatc tccaaagcca ttatttgttt ttcataatta ttatttcctt tcctaattta
    28621 tgttttttta taatatcaca acaatgttta ttgaaagttt atataatttt aagaacatct
    28681 aaaaatgcat acttgatatt ggtaaattag tgataggagt tcaatgtatg atattatata
    28741 ctaaagatga attttaatat ataactttga tataataagc atatgtaaat gtttaatatt
    28801 taattaaaga aaattatcat ataaaaataa atttgtaagg agcaatacag tatgaaaaaa
    28861 caatatgtgt ttataatact agcaagtact attttaggta tacttgttgg aaccatattt
    28921 agagattata gcaatgaaga aacagtttat acaagcaaag acaagtcaat aagaaaagaa
    28981 ataaaaatga caagaaaatc tataaaagaa ttaaccaaag agaaagacaa gttagataaa
    29041 gaaattaatt caatgaaaca aaatcaaaaa aattctgaag aaatatcaga aatagaaaca
    29101 cttagagata gtctatcata ttcagatgta aagggtagtg ggataatatt aagtatagat
    29161 gctatagatg aaaaaacagg gaatatagct aatagtatag actataataa gattcttgta
    29221 aaagttataa atgaattaaa agtaaacgga ggaaaataca tagaaataaa tggtcaaaga
    29281 attaatcaat attcagaagt tgtacttgca ggaagccata taaatataaa tggagttcct
    29341 gtagcacaac catatgaaat aaagtgcatt ggagataagg agaaattatc taattatgta
    29401 gataaaggta gtaaatattt agatagcata caaaccaatt atccaatgaa aataagcata
    29461 aaaactgaag aaaatatctc tatgaaaaaa atgaatatac ctaataaatt aaagtatata
    29521 aagggtgatt aatattatat tagaggaaag cagtttgcca atgttaatac cagaaaaact
    29581 tgcaaagaaa tataatgtat ttccagttga gtttcaagat gataatttag ttgtagaaat
    29641 agaagatgag gatatttatg ctctacagga cttgagatta gctacaggta aagaaattat
    29701 attgaagaag gagaaaaaag aacttataag tgaaaagatt gaaaaatatt ataatagctt
    29761 tgaacttgat gaggattatg caaaaaaatt atttgaaaat cttttagaaa aagctataaa
    29821 agaaaatgca agtgatatac atatagaacc atttaaagaa catttaatag tgagaatgag
    29881 ggtagatgga gaactaaagg aagtatcaaa gtttttagtt gatgtctatc catcattgtc
    29941 tacggttata aaattaaagg catcaatgga tataacagaa aaaaggcttc ctcaggatgg
    30001 tagggtggat ataaagttag gtggaaattt aattgacatt agagtttcat caataccaac
    30061 tgtctatggt gaaaaaatag ttttgaggat acttaataga gatacttttt taaaagacaa
    30121 attagaactt ggtttttcag aggaagctat aaaatctata aatcgtataa taaataaaag
    30181 agcaggagta ttattagtga caggtcctac aggtagtgga aagactacaa cagtatattc
    30241 cttattaaat gatttaaaag gaataaataa aaatattatg actatagaaa atcctgtaga
    30301 gtataagatg gatggcataa gtcagattca agtaaattca aaagtagggt tgagctttga
    30361 tgtaggtctt agagcgatat taagacaaga cccagatata ataatggtcg gagaaataag
    30421 agatgcagaa acagctaaga tagcagtaag agcagctata acaggtcatt tagttataag
    30481 tactttacat actaatgatg cagtatcttc catagctaga ttactagaaa tgcaaatacc
    30541 accatatctt ttaaatgcct ctcttatagg tgttatatct caaaagttgg ttagaaaagt
    30601 ttgtaatcat tgtagccatg agattatgat aaaagataat tttagtggag atgtaaatac
    30661 taaggtagct gtagggtgtg aaaaatgtaa tgataaagga tactttggaa gaacagcaat
    30721 ttatgaaatc ttagaaatta atgatgatat aaagacatgt ataagaaata tggaggattc
    30781 tagtactatt aaagatgtgg cacaaaataa tggaatgata acatttgaag atagctgtaa
    30841 aagactaata aatgaaaaaa taacgacatt agaagagtgt ttagtagtaa atgaaatgat
    30901 ttaactgaat tgtatagtta aacttcgttt ggtcagttgt ctttatttaa tctattttaa
    30961 ttaagatgtt tgagtataaa ttatatttaa gtgaaataaa attaaatgca ttaaattaga
    31021 aaatattaag tttatttaat tgtaattaat ataatttgga aggtattata gtacatgaga
    31081 ttatctaaaa atatatttta taatttagaa aagttttatt tagtattaaa tagaatcagt
    31141 gataaagaaa ttagagtaat ttgcaaagag ataggcatat tgctggaatc tggatgtgaa
    31201 attacaaaaa tatttgaaat cattgagagt cagtcaagta aaaaagtaaa gaatttgctt
    31261 agtatagtat caaatcatat tcaaaaaggt aactcaatag cagaatcatt tcaaataaca
    31321 ggtatatttt ctaaattctt tataagtatg ataaaagcag gagaaactag tggaaactta
    31381 gatataataa tgagtgattt atctaattat tatgataaag aatacaaatt aaaaatgaaa
    31441 ataataacta tttcaatata tccaataata cttataatat tatctatctt atcaatgttg
    31501 tttatattcg tatttgtaat acctaatttt caggtggttt ttaccaacaa tggaattgaa
    31561 ccacctttaa taactagagt tttaatggga atatctacag tggttacaaa taacttagct
    31621 tatataatat ttagttttat attgttttca ataggaagca tttatttctt tataacaaat
    31681 gataatataa aaaaattgat taattcgtta aaattcaaaa taccttttat aaaaaaaatt
    31741 aatcagttag tagctacaac aagattttca agggcattgt ccgttttaat tagcagtgga
    31801 atacaaattg tggaagctat agacatgtca gctagtgtta tagacaacga atttatttat
    31861 gaaaggttaa agatttctaa taattatata aaaaaaggaa atagcatagg taaatctttg
    31921 aatttagcaa atgtttttcc aaaattattt atttcaatgg caaatacagg agaagaaagt
    31981 gggaaactag ataaaagtct aaatgtaata aataaatttt atgaaaatga attaaataac
    32041 aaaatagagc aaataatgaa atttatagaa cctatgctaa ttgtatttat gggattaatt
    32101 attggcactt ttattttagc aatggttgtt cctatgtttg atgcaattac taatttttag
    32161 agagaaaaat aaattttatt agatgaaact tttgaaaagg agagaatatt taattgataa
    32221 atttgataaa taaaaaacga aaaggattta cacttgttga aatgattgta gtagtaacta
    32281 ttttaggcgt tatatctagt atagcattag ttaagtatag taaggttcaa gaaagtgcca
    32341 aattaaatgc agactatacg aatgctgcta atatagtaac tgcagctagc atggcaatta
    32401 atgatgatga aaagacaata gactctctaa gtgtagaaac attgaaggaa aagggatacc
    32461 taaatactgt tccagttcct cagagtacat caggtaaatt cgaacttgtc ataaatgata
    32521 gcggaacaga tataagcgta aatataaatt ctaaacaatt ttatccaaaa taatatttta
    32581 tattgattgc tgtaagtttt aaaattattt ttatttaaga aggagatggc tataattaaa
    32641 aaaattctaa acttagaaat aaatgatgat tatataaggc ttgtattttt aagaaaaagt
    32701 cataaaaaag tttttataga taaatcaatt ataaaagaga ttaactttga tataaaaaat
    32761 gatagtaatt cgattgagaa gattaaatat atagaagatg tagttagatt tgtaaaagag
    32821 gagattggta aatcaaaaat attatataaa aatttatact ttaacattca aattcaagat
    32881 atagtcatta gaaatgttga agttctaaat actaagaaaa aaagagacat attaagcatg
    32941 attgaatttg aaataacaca atacattcca ataaacataa ataactattc aataaaatat
    33001 aaagttataa attctaaaaa tgataaattg aatatacaag taattctttt cccaaagagt
    33061 attattcaat taattaagaa catttcagaa aatctaaata tgaatccaaa gactataaat
    33121 ataaattttg atatattaca aaagcttata aacttaaatt taatagagaa tttttcagaa
    33181 gaaggggtat ttatagaatg taaaaaaagt cagttaatta taaatataac aaaagataaa
    33241 aaaataagag aaacatactt gctatcaaaa gagggaaaat catatgagtt cataaaaaga
    33301 ttattaaggg attttaagta tgtttattac tatggtatag aaaattgttt tattgaagaa
    33361 tactttaaat atacagataa atctattgaa gtaaatccat taagattaaa agataaagta
    33421 aaagtagaaa ataattttga tgcagattta gaagacaata ggataaatta tataaatagt
    33481 ataggaatga ttatttaagt gaaagtggat aaaacaataa attttcttaa tggatttaaa
    33541 aaaactaaac catgtaataa aatctataaa ataataatag tcttaatttt aacgtgtatt
    33601 ttatttaaat catttatgta tgtgaaatct ataaatgaac ttgaagatta tataaatcaa
    33661 aataagctag tggataaatc aaagcacact ttaccaacaa cagataaaac tagagagaaa
    33721 ttttctaaaa taaaacttaa tgaaataaaa aaaatatatg aaacaattgg aataaatagt
    33781 attactaaaa tatcgataga taaaaataag ctagagatgg aaggtgtgtg tagtgattta
    33841 gatgttttaa acaacctaag gaaaataaag tacataaaat attattacat agacagtatt
    33901 gtaagagatg gagaatatta tagatttaga ataaaaagtt agataggtga ttatttttga
    33961 gattaaataa tagagaaaaa aatctactta aaatattgtt aatattagtt attatattgg
    34021 caagtgatat agcagtaagt tatttggaag aaaacctaac tagtttgaag aatgaaaagc
    34081 aaaatataat aaataaaata aataatcaag ataattatca aataaaggat aataagcata
    34141 tagatgataa atatatagaa agtgttgaat taaataaaaa tgaagaattg aaaaataatt
    34201 ttaataactc atttttagat aaaaaaacaa ataaagatat agtgttatta gtagaaaatg
    34261 aactgagaga tattgtgaat gtagaatata ttaatcaaga aagtagttta gataatcaca
    34321 ataaaaaaca agttaattta gaaataaaag ttaatggaga acttgcaaat gtgttgaaac
    34381 ttgaaggtgt aatgaagaag ttaagactaa ataataaact tgaaaagata gaaattaaca
    34441 aattaaaagt caataatttt gatgttcaaa atattccaaa tgaatataaa caagaaaaag
    34501 acaatattgt tgaatgtaaa atgacagtta gagtcatata acaaaaaaga atagataaag
    34561 taaaaaacta ttatcaatta ataaaatttt atagaggaat tagttttacg ataattcatt
    34621 tctacaagag ggattgttga tgattttatc gaaatcctaa ttaaagttaa tatattgtac
    34681 caaaatataa gtgaatttaa tagaatattt acttatacta tagattgatt aataaaaaaa
    34741 tggtataata acaaattgta ctttaattta ataataaggg agtggaagtc atattgttag
    34801 ggagaaatga gctatgccct tgtggaagtg gcaaaaaata caagaaatgt tgtttaaata
    34861 aagatattgt ttcagaaaga gcatgcagaa aaactgaact ttcacaaaaa caatatacag
    34921 atttatattc aaagttatat gattattcaa gacaggataa gtttaaagaa gaatgtgaaa
    34981 aagctaaaga aatgttttat ataatgcaaa atgatagtgt aaatgaaaaa tttgaaagat
    35041 tttttaatac ttatttaata caagaccaca taatggaaaa taaaaaggtt atgacagttg
    35101 gttttcttga agaaaatggt gcaaacctaa gtcaatcaga agtatcaata ttaaaaagta
    35161 tgtttgaatc atacgtaagt atttatgagg taaaagaagt ttctgctgga aaaatagtat
    35221 taaaggactg tttaagtgga aatgaacttt gtacagaaga tgtaaaactt ctgagaagtt
    35281 ttaaaatcgg aagttgtatg atagctagaa tagttgatat tgaaggaact aacatattga
    35341 tagatataac tataagtata tcaaatgagg ttaaagatat aattttaaag gatattatga
    35401 atctattcaa tcaatatcaa gatgtataca aagatatgaa gtcgttctta atttatcata
    35461 cacatatact atacaagtat atacaacagc ttttagaccc aagtatagct gaatatttaa
    35521 agagagaaag agataaaaac gatacaaaag ttaattcaga aaatgaatta gctgaagaag
    35581 aatgtaaagt tttagatact cttaaagaaa atgtagaaaa agaaaatata gatgcatgta
    35641 tagagttttg gaatgaatat aagaaaaatc atgaaaatat aaaaggagct gaaaatggtt
    35701 gggcagctgc tgttgaatac tatgttaaaa aagaagcagg acaacctata actcaagttc
    35761 aaatatcaaa gaaatacgat ataagctcta gtacactagg aaagagatac aaagatttaa
    35821 aaatatctta attaattata aaaagcttta agtcatagat aaagaatcta tagtataata
    35881 tatgtataat gtttagaaag aggtattaat aatgagtctt agtttaggaa atgcaattgc
    35941 tgtatcaacg aactttataa aatctaaccc agctgtacct gtgtacaacg gagaagaaat
    36001 atatgcagtt atttatcata tagaaatagg atataataat ggtgtatcac ctgataccaa
    36061 acaatatata ataatagact ataaagagtt tgatttatct atggataaaa aaaatgaact
    36121 aataaatgtt gctaaagagt actgtaaatc taaaggtatt gtagaaaacg aaaaccttga
    36181 aataaacctt ttagacaatg aagaagcaga gaacttctta gaaaaggtgt ttaataacat
    36241 gaaggttata agaggtctta taactaaaaa aggataaata aatggactat ataggaatag
    36301 aaaatataac accttatgaa aatacatatg aatttagtgt atatgaatat gatgatgaaa
    36361 tcaccttagg tagtgaaaag ttatatgtgt gtgaattaag ggttgtattg attaaagtta
    36421 attctctgta tgttgaaaga ttgcataaat cagttgaagc aatggtctta gtaaaaaact
    36481 tgaaaaagga tttagataaa acacttgttg taaacaaaat aaagaatttt gtgctagatg
    36541 agatttgggt agaaaatcta gtaaaagaga atatagaagt tatatttgta gaaagctagg
    36601 aataacccta gcttttttat attacaatct aagagaggaa aacaatatgt taagagtatc
    36661 taatgtaaaa cttggaattg ataaagatat atctttatta aaagatgttg tacttaagaa
    36721 gctaaaaata aaagaaaaaa acttagtcaa atatagtata tataaagaat ctatagatgc
    36781 tagaaaaaaa ggaaaaatgg aatttgttta ttctgtagat gtagaagtca aagatgaaaa
    36841 taaaatactt aactcaaaaa taaaagatgt aacaaaaata aaggaaatca gatacataaa
    36901 tgtacctgtg ggtgataaaa aattaaagaa taatcctttg gtaattggta gtggaccagc
    36961 tggtttattt gcaggtctgc ttttagctca aatgggttat gaaccaatca tacttgaaag
    37021 aggtcttgat gtagacagta gaaccaaaga tataagtgat ttttggacaa ctagaaaatt
    37081 aaaaaataat tcaaacgttc aatttggaga aggtggagca ggaacttttt cggatggtaa
    37141 acttacaact agaataaaag atatacgatg tagaaaagta ttagaagagt tagttcagtt
    37201 tggagcacca gatgaaatat tatactcaca taagccacat gttggaacag atatattaaa
    37261 aaatgtagtt aaaaatataa gaaatgaaat aataagttta ggtggtcaag ttagatttaa
    37321 ttcaaaagta acagatatag ttgtatcaaa tgattctata caatctgtaa taataaatga
    37381 tactgaaaaa atagatacag atacaattat actagctgtt ggacatagtg caagagatac
    37441 ttatgaaatg ttgtattcaa gaggtataaa aatagttcaa aaaccttttg caataggagc
    37501 tagagttgaa catccacaag atttaataaa taaagcacag tacaaggatt tttataatca
    37561 tcctaggtta ggagctgcag attatagact tatagagcat gtttcaaatg gaagaacagc
    37621 atatactttt tgtatgtgtc caggaggtag tgtcatagca tctgcatcaa atgattttga
    37681 aatagttaca aatggtatga gtgaacatgc aagagataaa ataaatgcaa atagtgcatt
    37741 tttagtaaat gtaacgccag aagattttaa gagtgataat ccactagcag gtgttcattt
    37801 ccagcagaag tatgaaaaat tagcttttga attaggtgga aaaaattaca atgcaccagt
    37861 tcagcttgtt ggagatttta taaacaataa gacttctttg aatttaggaa gtgttgagcc
    37921 atcatataga ccaggatata cacttacaaa tttaagtgaa tgtttgccta gcttcgtaac
    37981 agagactatg agagaagcgt ttatatctct tgataaaaaa ttgaatgggt ttgcaatgta
    38041 tgatgctgtt ttgacgggag tagaaactag gtcatcatca ccaataagaa tagtaagaga
    38101 tgaaaatact ttagaatcag tgagtattaa gaatctatat ccatgtggtg aaggcgctgg
    38161 atatgcaggt ggtatagtta cagcagcagt tgatggtata aaatgtgcag aaaaaataat
    38221 acaaaaatac accaataata caaaaattta acataaactt aatgattttt taatatttaa
    38281 catgaattta acattatcac tcctataatt tgataaaata caataggtta taaaattttt
    38341 agatacagta atctctttac aaggaggaaa taatttgaac attataataa gtctaatggg
    38401 tggattaggt ctgttcttat atggtatgaa ccttatggga gaaggtcttc aaaaatctgc
    38461 tgggacaaag ctaaaaaaga taataaaact acttactagt aacctattta tgggagtttt
    38521 ggtaggtaca ggagttacag cagtaataca aagttcaagt gctactactg taatggttgt
    38581 tggtttcgta aatgcaggaa taatgacttt aaaacaggca ataggagtta taatgggtgc
    38641 taatataggg actactgtta cagcccaatt ggtatctttc gacttaactg gtatggcccc
    38701 agtagcatta ggtgtaggta ttatacttta tttatttgga aataagccta gaataaaaaa
    38761 tatagccgaa atacttatag gttttggtat attatttaca ggtatggatt ttatgaagat
    38821 ggcagtggag ccactaagag actatcaagg atttactgac ctacttgtta catttggtag
    38881 atatccacta cttggattat tattgggatt tgggattaca gcaataatac aaagttcaag
    38941 tgcatctatg ggtatgttgg ttgcactggc agcagaaggt ctagtgccat tatcagcagc
    39001 actgccaatt ctttatggcc aaaatattgg tacatgtgta acttcactgt tatcaagtat
    39061 tggtgctaac aaaaatgcac gaagagcagc tatgatgcat ctaatcttca atgttttagg
    39121 aacggtgata ttcttaatat tcttgaataa accagtagta agcatggtta caagttggga
    39181 tccaagcaat gttgcaagac aaatagccaa tacacataca ttgtttaata taatatctgt
    39241 attgatactt ttaccattta caaatcttat tataaaacta gctataaagc ttgttcctga
    39301 tagagcaggt gatatagatg aagatgaaac taaaactatt aaatatatag atgatagaat
    39361 gatagaaact ccttctatag ctcttgcaaa tacgataaaa gaagcacttc gtatgggtga
    39421 gaaggcgaag gaaagcctaa atgcatctat ggaagcctta gtagaacatt caactgaaaa
    39481 aatagctaag acatatagac gagagagatt aatcaatgat ttacagaaag ctattttaaa
    39541 ttacttactt aaactttcaa aagcaccttt agatgatgat tcaagagaag ttgtggatac
    39601 tttgtttaat acagtcaatg atatagaaag aattggtgac cacgctaaaa atatagctga
    39661 attatcgcaa gttgcaatag attcaaatat ttctttctca gaagaaggac aatctgaact
    39721 tgatgttatg tataatagag ttgtttcagc atatacttat gcattagaat caatgagaac
    39781 tgataatgtt gatttagctt gtaaggttat aaaaattgaa gaacaagttg atataatgga
    39841 gaaatcttgt agagctaatc acatgtatag attgaacaat aatctatgta gtatagaaaa
    39901 tggagttatt tatttagacg taataagtaa tttagagaga atatctgacc atgctgtcaa
    39961 tatagcacaa caagttatag cgaagagatt aggaaatgat taaaaataat taatttaaaa
    40021 taatataaaa tttaaaatag aggataaata aaagagaaac tttaaatgat acgtttagag
    40081 tttctttttt tgtaaattta tttgactaaa atttttaaat ttatacatag ttatagtatt
    40141 tagaagaatc gatttcctga taataaatga tgagatatgt atgattttgt ggcaaacttt
    40201 taaaaaaaac acctatattt tgtgttataa tctctttaaa acgaatatgc tgaaaagagg
    40261 gatttaaatg ggaaaaataa attttgatta tagtaaagct acagagtttt tttgtcaaaa
    40321 cgaaatagat gtcatgcaac catatgtaga tgttgcgcac gacatgttac ataataaaac
    40381 tggtttagga aatacttttt taggatggat agaccttcct aaaaattatg ataaagaaga
    40441 gtttgacaga ataaaaaaat ctgctgagaa gataaaatca gactcagatg ttcttctggt
    40501 aataggtata ggaggctctt atttaggttc gagagctgct atagatatgg taagtcattc
    40561 ttttaggaat ggacttaaaa aagagcaaag aaaggctcca gaagtatatt ttgtagggca
    40621 taatataagc tcaacatata ttatggattt attagatata attgaaggaa aggatatttc
    40681 tgtaaatgta atatctaaat caggaactac tacagaacca gctctggctt ttagaatatt
    40741 taaagattac ttagagaaaa agtatggtaa ggaagaagca agaaagagaa tttatgcaac
    40801 aacagatgca agtaaaggtg cacttagaca gttagctaca gaagaaggat atgagacttt
    40861 tgtaatacca gatgatgtag gtggtcgttt ttctgtattg acagcagttg ggttacttcc
    40921 tattgctgct gctggacttg atattgatgc tatgatgaaa ggcgcaaatg atgctagaga
    40981 agcatttcaa aatcctgact taaaaagtaa tgatagttat agatatgctg ttgctagaac
    41041 aattttacat agaaaaggaa aagatgtaga gttattagtg aactatgaac ctcaactaca
    41101 ttatgtaagt gagtggtgga aacagcttta tggagaaagt gaaggaaaag aaaataaagg
    41161 tttattccct gcttctgtag atttctcaac agatttacat tctatgggtc aatacataca
    41221 agatggaaaa aggcttttat ttgagacagt acttaatgtt gaaaactgta agagaaatat
    41281 aactataagt tcagaggaag tagacttaga tggtttaaat tatttagcag gtaagactgt
    41341 agattttgta aatcataaag cttttgaagg aactctattg gctcatacag atggaaaagt
    41401 acctaattta gtaataaata tacctcaact agatgagtat aattttggat atttagttta
    41461 tttctttgag aaggcttgtg gaataagtgg atatttattg ggagttaatc catttgacca
    41521 accaggagta gaagcttaca agaaaaatat gtttgcttta ctaggcaaac caggttatga
    41581 aaaagaaaag gaagagctag aaaaaagact taaataattt taaatagtta aggtttaatt
    41641 aagattaaaa taatataatg aaatcatcga aaaggagttt actcaaaata gatgatttca
    41701 ttttttttat gaaaaattgg gaaaaatata ttttgataat ttatattata ttaagatagg
    41761 gagtggtatg agatgtcaag aagaaagaaa gggataagtt tagtaatatt agttgctata
    41821 ataagttcaa tacttagtag ttttctaaca ataattttag ttaaggacaa tttagtaagc
    41881 aaatctacag gaagttctac gccgatagtt gtaaatgatg atggtaagag tcaaaatatt
    41941 tatcaagcag ttgcagaaaa agctacgcca tctgttgtag gtattacaac aactagtgtt
    42001 gatactagta atatgtttgc aataccaact gaaacacaag gtgtaggaac tggaatcata
    42061 gttgattcaa atggatatat acttactaat tctcatgtta tatcagatgg tcaagctaca
    42121 agtgtaaatg tattattcaa tgatggttca acaacttcag gtaaagtagt atggtttgac
    42181 caacaacttg atttagctat agttaaggtt gataaaactg gactaacacc agcagaattt
    42241 gctgatagtg acaaagtaaa agttggagat atatcaatag caataggtaa tcctcttgga
    42301 ttagattttc aaaaaacagt tactcaaggg ataataagtg gattagatag aactatacaa
    42361 acagaaaaaa ctaatatgac tggtttatta caaactgatg caagtataaa tgcaggaaat
    42421 agtggaggac cattgttaaa tcaaaaaggt caagtaattg gtataaacac agcaaaagct
    42481 tctcaagcag aaggattagg atttgcaata ccaatcaata cagctaagag tatagttgaa
    42541 gaagtaatca aaaatggaaa atatgaaaaa gtaactttag gcataaaagg tacagatgta
    42601 tcaaattatg aagcagcaac aggtacaaaa ttaagtactg ataaaggtgt ttatgttgca
    42661 gaagtaatat ctggttcatc agcagaaaaa gctggagtaa aggtagggga tattataact
    42721 aaagtaggag atacagatat aacaggtatg aatgatttaa ataaaaagtt gtatactttc
    42781 tcaaaaggtg ctagtacaaa aataacagta aatagaggcg gaaaagcagt aacaataaat
    42841 gtgaatttct aaatatgacc agaaagaagg agactacatg gtaaagtgta gtctctattt
    42901 ttatttaatt tttatagtat atacatctaa attaattaaa atgatgtatt gtttatagtt
    42961 tattgctata ttttcttata aaaataaatt taataaatat ttaaatttaa tttacaaaaa
    43021 caatataaat atcataataa aataaaattt tttaatatta ctaaaaatat atagacaaaa
    43081 taataacaaa tatagtatta tgtaattgta gaaatgcttt tcataggtat attctatgaa
    43141 gatgaattgc ataaaaatga aaaaatggaa tggatattat ttaggggtga aaaatcatga
    43201 atccattagt tataaactta caaaaatgta gcatacatga tggaccaggg ataagaagta
    43261 cagtattttt taaagggtgt ccacttgaat gtgtttggtg tcataatcca gagagtcaaa
    43321 catatacaaa acaagttttg tataatgaag aaagatgttc aaaatgtgaa gcatgtataa
    43381 atatatgccc tcataaggca atttacaaag gtgaaacaaa aatatgctta gaccaagata
    43441 agtgtgagtt ttgtgaaact tgcttagact attgtgtaaa caatgcaaga gagattgttg
    43501 gtcaagagta ttctgtaaga gacttggtta aagagataga gaaggataga attttctatg
    43561 aagaatcggg tggtggagta acactatcag gtggagaagt aatggctcaa gatatggact
    43621 tcatttgtgg agtaataaac atgtgcaaat caaagggaat ccatgtagca attgatacat
    43681 gtggctatgc aaagtcagaa aattatgaaa gagttgctaa atgtgcagat ttgtttttat
    43741 atgatattaa gcttattgat gaggataagc acataaaatt tacaggaaaa tcaaatgact
    43801 taatactgaa aaatgttaag attttaagtg aattaggggt taacataaat ataagaatac
    43861 cattaattgt aggagtaaat gtagatgatg aaaatttaga ggttaaaaaa atgatagagt
    43921 ttttaaagcc tttgaatata caagcagtaa gtcttcttcc atatcataat attggtaaac
    43981 acaagtatga taaaatttat aaaaagtatg aaggagaaga actacaaaga ccatcagaag
    44041 aaaaattaga ggaaataaaa agactgtttg aagcaagtaa tttcaatact aagattggag
    44101 ggtaataaaa tggcaagagg aacttttgag agaactaaaa aattaagaga agaaagtatt
    44161 aatgcagaac cacacatatc tattgaaaga gcagtactta tgacagaagc ttataaaaaa
    44221 tatgagggaa gtgtagagat tcctgtactt cgtgctttat catttaagca ttatattgag
    44281 aatagaactc ttagcataaa tgatggagaa ttaatagtag gtgaaaaagg tgattctcct
    44341 aatggagcac caacatatcc agaaatttgt tgtcacacaa tggaagattt agaagtaatg
    44401 cacaatagag atataataaa ttttagcgta tcagaagaag ctagaaaaat acataaagaa
    44461 gaaataattc ctttttggaa aaaaagacaa acaagagata agataataaa tgcaatgaca
    44521 cctgagtggt tagcagcata tgaagcaggt atgtttacag agtttatgga acaaagagcc
    44581 ccaggacata cagtttgtgg agatacaata tataaaaaag gatttttaga tttaaagaaa
    44641 gacatagaag caagattaaa agagctagat tttcttaatg atttagatgc atataataaa
    44701 aaagcagatt tagaagctat ggcaatagct tgtgatgcaa tggttatatt aggtaaaaga
    44761 tatgctgaga aagcaagaca aatggcagaa gaagaaacag atgaagctaa gaaaaaagat
    44821 ttattactaa ttgctgaaac ttgtgatgtt gtaccagctc acaaaccaga aacttatcat
    44881 caagctatac aaatgtattg gtttgttcat ataggggtta ctactgaact taatatatgg
    44941 gatgctttta ctccaggaag acttgaccaa catctaaatc ctttctatga aagagatgta
    45001 gaaaatggaa tactagatag agatagagca caagaattac ttgaatgttt atgggttaaa
    45061 tttaacaatc agccagcacc accaaaagtt ggtataacat taaaagaaag tagcacatat
    45121 acagattttg caaatataaa cactggtgga ataaatccag atggtcaaga tggtgttaat
    45181 gaagttagtt atataatact tgatgttatg gatgaaatga aattgataca acctagttca
    45241 aatgttcaaa taagtaaaaa gactcctcaa aaattcttaa agagagcttg tgaaatatca
    45301 agaaaaggat ggggtcaacc agcattttat aatactgaag caatagttca agagcttatg
    45361 gaagctggaa aaactataga agatgcaaga cttggtggaa ccagtggttg tgttgaaact
    45421 ggatgttttg gtaaagaagc atatgttcta actggatata tgaacattcc aaaaatactt
    45481 gaattaacat taaataatgg atatgaccca attagtaaaa aacaaatagg tattgaaact
    45541 ggagacccta gaaatttcca atcttatgag gaactatttg aagcatttaa aaagcaatta
    45601 cattatatga ttgacataaa aatagaagga aatgctgtga tagaaaatat atgtgcaaaa
    45661 cacatgcctt gtccacttat gtctacaata gttgatgatt gtatagaaaa aggcaaggat
    45721 taccaaagag gtggagcaag atacaacact agatatatac aaggtgttgg tataggaact
    45781 ataacagata gtttaacagc aataaaatat aatgtatttg ataagaaaaa attcgatatg
    45841 gatacattgc ttaaagcact tgatgctaat tttgaaggat atgaagctat acttaattta
    45901 gtttcaaata agactcctaa atatggaaat gatgatgatt atgctgatga aataatgcaa
    45961 gagatattca atgcatatta caatgaggta actggtagac taactgtttg tggtggagaa
    46021 tacagagtag atatgttacc aacaacttgt catatatact ttggagaaat tatgggagca
    46081 agtccaaatg gtagactttg tgcaaaacca gtttcagagg gaatttcacc tgaaaaaggt
    46141 ggagatacta atggacctac agcagttata aaatcttgtg ctaagatgga ccacataaaa
    46201 actggtggaa cattattaaa tcaaagattt gcaccatcag tagttcaagg agaaaaaggg
    46261 ttagataata tggctaatct agtaagagca tactttaata tggatggtca tcatatacaa
    46321 tttaatgtat ttgataaaaa tgtactgtta gaggctcaaa agaatcctaa ggattataaa
    46381 gacttaatag ttagagttgc aggatatagt gaccatttca ataatttaag tagaacacta
    46441 caagatgaga taataggaag aactgagcaa acattctagg tttgcttgta aataaaactt
    46501 cttactatat aaaatcgtaa ctgatataat aaagtataaa tggtttgatt ccatttttaa
    46561 aattttagat taattatgag ctaactttct aaataggaag ttagctcgtt aagtttaatg
    46621 aaaaaatcgt taaatttata tttagtaaat catacacata ttagtaagta atacatacaa
    46681 aagaataatt tattatagtt ataataaggt tatattttat aaaaataaga taattttaag
    46741 cagttaaaat aaagtcaatt tagctacttt aaaataaaac taatagaaaa aaggagattt
    46801 gatatgaaaa ctttaggatt tattggttca gggaatatgg gaagtgcaat gataggtggc
    46861 atagtacagt caggattagt aaaggcagaa gatataactg tatcagattt aagccaagtt
    46921 gccttagata aagtaaaaga agaatttgga ataaatacta caactgattc aaaggtagct
    46981 gttacaaatt ctgatgtggt aatagtagca cttaaaccta atatatgcga aaaggcttta
    47041 actccactta aagaattaat agatgcaaat aaaataatag tatctatagc agcaggtaaa
    47101 actatagaaa gtctggaaag ttttataggt tcagataaaa aaatagtaag agtaatgcct
    47161 aatactccag cccttgttgg cgagggaatg tcagctcttt gtaaaaatag taatgtaaca
    47221 gatgaagagt taaatatgat taaggctctg tttgaatctt ttggtgaggc agaaatagta
    47281 tcagaatatt taatggatac agttacaggt attagtggtt cttctcctgc atatgtattt
    47341 atgtttatag aagctatggc agatgcagca gtattagctg gaatgccaag acaacaagct
    47401 tataaatttg catctcaagc agtaatgggg tcagctaaga tggttcttga aactggaaaa
    47461 catccaggtg aattaaaaga tatggtttgt tctccagcag gaactactat agaagctgtt
    47521 aaggtattag aagaagaagg ctttagagca gcagttataa aatctattgt tgcttgtata
    47581 gataagtcta aagatatgag caaataacag atgtaattaa gtttactata tggtttcttg
    47641 aattgttttt ttagtactta tttatatcta tacataaatg tatttggtat aagtaaaata
    47701 agagtaattt actataaaaa ggaatttaat gattgagtga gaatttatta cttaaatatt
    47761 aataattaaa tagatatatt tttaactata tttgagatat atcattgtga aaaaataaaa
    47821 tttataagta ttattgtgag gttataagta gattt
//
LOCUS       DAELXX010000016        46623 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.016, whole genome shotgun sequence.
ACCESSION   DAELXX010000016 DAELXX010000000
VERSION     DAELXX010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46623
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.016"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            24..716
                     /locus_tag="KPH29_000888"
     CDS             24..716
                     /locus_tag="KPH29_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1466515.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            726..1730
                     /locus_tag="KPH29_000889"
     CDS             726..1730
                     /locus_tag="KPH29_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1466516.1"
                     /translation="MKLFLKDYKGYIFIYYIGIVMTIIYCNLMKFISFGESLYILLFS
                     SFILLCFLIYKYYKNREVYNLFEKGIHSLEESFLYLGSSFLGRNISVILKQQHKLYQS
                     MIQEHKKIHSEHLTFINQWIHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLD
                     MAMHFARADNFQKDFIVEKAKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDV
                     KWMKFIIGQLITNGVKYSKDYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFDP
                     FFTGENGRKFGESTGMGLYLVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKDVNSLIKM
                     "
     gene            1978..2829
                     /locus_tag="KPH29_000890"
     CDS             1978..2829
                     /locus_tag="KPH29_000890"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBG1466517.1"
                     /translation="MSLDVIFILKSVIIAIVEGFTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVLA
                     FTNIISV"
     gene            2933..3679
                     /locus_tag="KPH29_000891"
     CDS             2933..3679
                     /locus_tag="KPH29_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1466518.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALFRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     SIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            3679..5595
                     /locus_tag="KPH29_000892"
     CDS             3679..5595
                     /locus_tag="KPH29_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1466519.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVVMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTYFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMMFIENNLKKENVDYTRTNGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     VKPISFEDNECISLLSSSLPGNNNVKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFNNESGMQPYLFFSKELMIGT
                     GKLIYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYVVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFLIVR
                     NNYLKEIRLNLVNF"
     gene            5874..6791
                     /locus_tag="KPH29_000893"
     CDS             5874..6791
                     /locus_tag="KPH29_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBG1466520.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSILDMINSQLEYNNDIYKNEFNNIVEKIKAVLN
                     TGEKSDTYMDYFTVQAQRNCDYKSEKNKCNEIIRAYTTKHKFWIEYYSLNSGNSERIV
                     HPYGLFNYKSDTYMVAFCEKRFKFIDFKLCRIKDYKVLEEKYNVDKSFSWDEYSKNSI
                     GIYKGEEINVVIKISHPFSIIIKEKVWVNNQQIIEYDDKSIMFKAKMRGYEEIKSWIL
                     SMGAYVEVVEPDRLRNDILSEIEKMKKIY"
     gene            6869..7606
                     /gene="cas6"
                     /locus_tag="KPH29_000894"
     CDS             6869..7606
                     /gene="cas6"
                     /locus_tag="KPH29_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBG1466521.1"
                     /translation="MKMKIEFSTGCIPISYNSLFMSIIKEAIRKSNEDYYKNMYYYKE
                     KNNKKTKNFTFSVYIKKYSIEGDNFIIEDKVILNISTPDLELGFHIYNGLMTSKKCLY
                     KAYELTRIRIDLSREKKVTEERVLFNALSPICVKSKEGKFLEITDDRYIEELNYITNE
                     VVKNYRGNGLKRKLEFENIGLKKIVVKESLREFKKITGKEYQYINGYKGKFILKGDID
                     DLNLIYNLGIGFRRAQGFGDVDILEWR"
     gene            7612..9489
                     /gene="cas8a1"
                     /locus_tag="KPH29_000895"
     CDS             7612..9489
                     /gene="cas8a1"
                     /locus_tag="KPH29_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas8b1/Cst1"
                     /protein_id="HBG1466522.1"
                     /translation="MKLRVYRGDWFFNMGIVGFLNIIKKADKQAEIFIMEDYIEFDSL
                     FLENFHEYYFNYFMDEYDVSKRIKKNIDYSINFIKSKPDRIKDGIKKIKDSVKQQNDK
                     IKKFDEEKFNLIKEKLDSMSKIKSYDEFDSLEALVDETIDIFKIKSINDRLTANLYKY
                     VVQDNYFGQVSFFNVAKAKLDLDGLKQIMFNDYLRQIIYFGELADLLEENDYDKLKNY
                     LNDRLNSIAKDINEKKVSKSSINTIEKIMKEINKNFIKKNKSIEDIKEYMDSLEVCEM
                     CGLYKGILDEYSESNFAPLGVSTNNARNMFWNQAYVPSICDICKLILFCTPAGATYTR
                     KNYLINEENEFYLFVNMDTSINELFERNNSLKAQKSEYSDSKDENPFNQLIKSIVEEN
                     TLKSEWQLRNILFVEFKASIDSKKCKINYFNIPTYLAKFFTDKYANKKIQSIYDYKLK
                     SNVLDLLLKNRDLKHLTNNILRNKVKNDMESNNKSNISGIDCFRVVQLRALINSYKKG
                     VYKMDSKNLEKNDEKLRIIYYLGCDIHDYFVNKNSKNKIDGVSYKLLNSVKVGNKSDF
                     MDTIIRVFMSAEKQIPAFILDIEIEKDLDFESIGHAFISGLISGKYEGKDKLPNEKEE
                     K"
     gene            9491..10405
                     /gene="cas7i"
                     /locus_tag="KPH29_000896"
     CDS             9491..10405
                     /gene="cas7i"
                     /locus_tag="KPH29_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HBG1466523.1"
                     /translation="MKRGLTFTVIAQAQSLNYGEGIGNISELKKLSRDDGNIYTFASR
                     QCLRYDIVRLAAELFNWNLQVVDKEKGTIQFKDNISIRESEEMDLFGYMKTNKKDEKD
                     KKGGSLTREATVRLSNAISLEPYRSDMDFLNNKGLADRIGEHPNLANIEQHLSYYTYT
                     VTIDLSKIGKDGDIELDNKEKCRRVVEFLEIIKVLNRNIRGRQENLSPLFVVGGVYEI
                     ANPFFLGRIKLKGDKNGFKINKQAIEEVIQGTFLGKDLKEFTYVGMVDGVFINKEEFK
                     GLFEDNFLSVDKFFGQLVKEVKEYYGVN"
     gene            10407..11108
                     /gene="cas5b"
                     /locus_tag="KPH29_000897"
     CDS             10407..11108
                     /gene="cas5b"
                     /locus_tag="KPH29_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5"
                     /protein_id="HBG1466524.1"
                     /translation="MKVLKLDLFQETACYKKPFAMKITETYPLPPYSTINGLIHKILN
                     ATEYIPFNISVQGTYESIFNNYQTTYFYKKDSITSMPMNSHMLLNVNLIIHIGGDFDL
                     LEEIYDSLLNYDEHLSLGRKEDLVRINDIRFVEVNKFEVDEDIQLDDYENYKLSECNI
                     RMPIYIPKTSLQDTDIDGISYRLNNYYDNNAVEDKKRVWNKVDSYYVEDGNTISIGDI
                     LLDNEGDLLYFNNIN"
     gene            11119..13440
                     /locus_tag="KPH29_000898"
     CDS             11119..13440
                     /locus_tag="KPH29_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBG1466525.1"
                     /translation="MLYAKSNPVETLREHTDELLKQMNVLRESYGKNINSLDFLEEEI
                     FWQLLDFVIEFHDIGKAFSPFQELIKSRMDTKINEPKIVTHLENNVGHNYLSPAFIDY
                     SYIDRKKNKELRAVLNQVIVYHHERDIFIDKDFKNLIQKILDEDLINKVYELQREFKV
                     RYPIKTEKLSKVYLQSVEKRIDKNHKYYNLYIMLKGILHRLDHSASAHEVVECNNVIN
                     IGEQTENYLMKEFGSLREAQSFAKSNRNKNIILIASTGMGKTETALIWIDKDKAFFTL
                     PLRVSINALFDRAKNIIGVGEANDTFLGLLHSTAIDYLEESNQENSSEIVDLAKLLSC
                     KLTFSTIDQIFKFPFLYRGYEKVYSTLAYSKVVIDEIQAYSPEIAAVLVKGIEMIHKI
                     GGRFMIMTATMPTIYIDELKKRGVMNSNLADLTCNTEKIRHCVSIVENSIDENLGKII
                     QSGMNKKVLVIVNTVKSAVEKYELIEEIVKPKGIDVNLNLLHSMYIQEDRAKLEKYIK
                     EFADSDSNGIWITTQLVEASLDIDFDELHTENSTLDSLFQRFGRCYRSREYEENSPNI
                     FIYTEKATGIGSIYDKDIVEKGLELLKTFINGKECVKMKEKYKVEMVKILYSKESLEG
                     TAFEKRFTSALNILDTITPYSLGSKDAQNILRNIEGYTVIPEDIYNKIEETLIKDYEE
                     LGQELINAYSNDDRQLINKIKSKRKKARREIIKKTVNLPIYKAKQNVIDIQVKGLEDL
                     KILLYKYDIHENKHTKQIFGKGVLISNEIDQFI"
     gene            13455..14006
                     /gene="cas4"
                     /locus_tag="KPH29_000899"
     CDS             13455..14006
                     /gene="cas4"
                     /locus_tag="KPH29_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HBG1466526.1"
                     /translation="MWLKFTKEGDFVPIDLEVLKVQGVKINYYYVCKRKLWLFSKGIT
                     MEQNSSRVLSGKIVHEDSYKRMKNKEMLIDDILKLDIIKDEYVKEVKISSKMKEADEM
                     QLYYYLYYLKNSMGIEKKGTINYVKEKKQEELILTEEKEKIIENTLININEINMQLSP
                     PKIEKKHYCTKCAYYEFCFVKEE"
     gene            14009..14989
                     /gene="cas1b"
                     /locus_tag="KPH29_000900"
     CDS             14009..14989
                     /gene="cas1b"
                     /locus_tag="KPH29_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1"
                     /protein_id="HBG1466527.1"
                     /translation="MYKDYYIISNGNIKRKENTIYYYNLEGEKKILPVEQVDDIHIYG
                     EVDLNTKLINYISKYGVMLHFYNYYGYYSGTYYPRKKNVSGFSLVCQSACYLDKNERL
                     YLAKSFVESASYHILRNMRYYKVDESLIDTIKDELNDLNDVKEISELMGKEGRIRKTY
                     YKSFNQILKDDFEFYKREKRPPKDPVNTLMSFGNSIMYTNVLSEIYKTQLDPTISFLH
                     EPSTKRFSLSLDIAEIFKPIIVDTIIFSLINNKRINKNDFEYQDKICYLSEKGKKKFL
                     QEFENKMQTTIKHRNLNRKVSYRMFIRLECYKLIKHFINDERYKVLKVWW"
     gene            14992..15258
                     /gene="cas2"
                     /locus_tag="KPH29_000901"
     CDS             14992..15258
                     /gene="cas2"
                     /locus_tag="KPH29_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HBG1466528.1"
                     /translation="MFVIVTYDIVEARSLNRIRRILRKYLTWTQNSVFEGNITDGKLH
                     KCISEIENIIDNSEDSIYVYEIKNPNSIKKKCYGIDKYSDEMFI"
     repeat_region   15442..17059
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=15442..15470
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
     gene            17249..17677
                     /locus_tag="KPH29_000902"
     CDS             17249..17677
                     /locus_tag="KPH29_000902"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBG1466529.1"
                     /translation="MIKKLNNKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKSFIGALFIEPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
     gene            complement(18133..18912)
                     /locus_tag="KPH29_000903"
     CDS             complement(18133..18912)
                     /locus_tag="KPH29_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466530.1"
                     /translation="MKVNENNINNIDEELDELSRKVYLESPDFWISISDKIIDKVFDE
                     MLLFFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYL
                     LSLNKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDIT
                     YKFSSKEAEPIEMSSEVSSIRCCNCDELLEDITLNKLYNLCKIQNCNNCGSNLTKVGI
                     IAKKKMNFDENTEKFKDVSTSYCCGNCDSEINLSQREHFKI"
     gene            19236..19781
                     /locus_tag="KPH29_000904"
     CDS             19236..19781
                     /locus_tag="KPH29_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBG1466531.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
     gene            19813..20949
                     /locus_tag="KPH29_000905"
     CDS             19813..20949
                     /locus_tag="KPH29_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HBG1466532.1"
                     /translation="MKEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CNLIIAGGFESSSLQPMRTYHKNDKRYNTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIERADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     PILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKTKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIERYNIFGGALAYGH
                     PYGASGAIITLHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
     gene            20953..22344
                     /locus_tag="KPH29_000906"
     CDS             20953..22344
                     /locus_tag="KPH29_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HBG1466533.1"
                     /translation="MNYYELLRIKKERHKNKNFLIIDGEKYTYENILSYSEQLGKQIS
                     LGENISILIYSKNIMFQLISFFAINYSKNIPIICHYNLSKKVLDDILLKNNISIIISD
                     ESMDVIDLYDDNNNEVAKKVNFSIFNPKFNLYMYQYKNLINYLDKSICMGALSSGSTS
                     VPKVLYRTYESWAGFFPIQNGIFNISGESILFVNGTLSFTGNLNSIMSVLYEGGSIVI
                     SSGLNCKSWIRTIKQYNVTNIYLIPTKLQLLVKHLKEPVLKVVSIFTGSQLLFEYTAR
                     NLKKYMPKSEIILYYGASELNYITYLCYDELIEKPLSVGRPFPGIDIYIRGGKIFVNT
                     EYAVYNATKPYSVNDIGYYDNDGYLIFEGRSDDIVNIGGFKVSSAKIENEVKKIPQIE
                     DAIVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMKDNLMKNEIPKKIIFLNSFPYTS
                     SEKIDRLALLKML"
     gene            22420..23295
                     /gene="dpsA"
                     /locus_tag="KPH29_000907"
     CDS             22420..23295
                     /gene="dpsA"
                     /locus_tag="KPH29_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HBG1466534.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEVGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            23298..23888
                     /locus_tag="KPH29_000908"
     CDS             23298..23888
                     /locus_tag="KPH29_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HBG1466535.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(24096..24296)
                     /locus_tag="KPH29_000909"
     CDS             complement(24096..24296)
                     /locus_tag="KPH29_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466536.1"
                     /translation="MKKLKTISIFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
     gene            24800..27454
                     /gene="adhE"
                     /locus_tag="KPH29_000910"
                     /gene_synonym="adhC"
     CDS             24800..27454
                     /gene="adhE"
                     /locus_tag="KPH29_000910"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBG1466537.1"
                     /translation="MAHKEQVIIENKKENEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYTQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDESADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDNAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRSPGKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKHTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEEPKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
     gene            27730..29949
                     /locus_tag="KPH29_000911"
     CDS             27730..29949
                     /locus_tag="KPH29_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG1466538.1"
                     /translation="MTGRNKSFTLITILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAVQGSQLAQRQIEKDLDVLNIFSNYYALNQDIPNEEKMKNLLDEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYVDEVNKSIKKITISVPLLNNDK
                     VVGVLYCTYNINTLMKLINVSFYENNSISYVVRNDGTIILHPQEDSLSKNIYKLLKQD
                     NDIREINRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSEQIINRAIYAVLFIILIFIAIIFYIIYIKKSNEKEILSLAYEDKVTHIGNQ
                     NKFYRECSKYLLDKPSLNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEELTE
                     GEVYARLSSDYFAIFCDYKNGKNKRIRKIDSIRNNIESNLSIVFEISLCVGIYFVEEG
                     EVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVY
                     YQPKFSLVTGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFERVCND
                     LSEWKKQGKKIVPVSINLSRVELYQPDIVKFINKTIQMYNLSSDLIEIEITETVAINE
                     LNILKNVLNELRKYGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLNGIEHDERSR
                     NIAKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            30203..30865
                     /locus_tag="KPH29_000912"
     CDS             30203..30865
                     /locus_tag="KPH29_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466539.1"
                     /translation="MIDVSIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            31235..32644
                     /locus_tag="KPH29_000913"
     CDS             31235..32644
                     /locus_tag="KPH29_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBG1466540.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKASGKTVEQVNDLLTSSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMITLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     SLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGGIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            32759..33034
                     /locus_tag="KPH29_000914"
     CDS             32759..33034
                     /locus_tag="KPH29_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HBG1466541.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DITVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
     gene            33664..33975
                     /locus_tag="KPH29_000915"
     CDS             33664..33975
                     /locus_tag="KPH29_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466542.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VRKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            33977..35902
                     /locus_tag="KPH29_000916"
     CDS             33977..35902
                     /locus_tag="KPH29_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBG1466543.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPLENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFDCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVVFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            35905..36396
                     /locus_tag="KPH29_000917"
     CDS             35905..36396
                     /locus_tag="KPH29_000917"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBG1466544.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            36448..37011
                     /locus_tag="KPH29_000918"
     CDS             36448..37011
                     /locus_tag="KPH29_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HBG1466545.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            37026..38003
                     /locus_tag="KPH29_000919"
     CDS             37026..38003
                     /locus_tag="KPH29_000919"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HBG1466546.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            37990..38313
                     /locus_tag="KPH29_000920"
     CDS             37990..38313
                     /locus_tag="KPH29_000920"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBG1466547.1"
                     /translation="MLMYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVED
                     EYAIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGAD
                     ILFKE"
     gene            38430..40208
                     /locus_tag="KPH29_000921"
     CDS             38430..40208
                     /locus_tag="KPH29_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBG1466548.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            40205..41578
                     /locus_tag="KPH29_000922"
     CDS             40205..41578
                     /locus_tag="KPH29_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBG1466549.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            41584..42252
                     /locus_tag="KPH29_000923"
     CDS             41584..42252
                     /locus_tag="KPH29_000923"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBG1466550.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            42529..43578
                     /locus_tag="KPH29_000924"
     CDS             42529..43578
                     /locus_tag="KPH29_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG1466551.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTYNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            43674..44663
                     /locus_tag="KPH29_000925"
     CDS             43674..44663
                     /locus_tag="KPH29_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG1466552.1"
                     /translation="MSYLKINNVFKSYDQKRVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGTIILQDKDITNLEPSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKVSKEKIDNKVKEVLETVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDVSIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            44668..46386
                     /locus_tag="KPH29_000926"
     CDS             44668..46386
                     /locus_tag="KPH29_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBG1466553.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLLTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAAALAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(46476..>46623)
                     /locus_tag="KPH29_000927"
     CDS             complement(46476..>46623)
                     /locus_tag="KPH29_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG1466554.1"
                     /translation="VEENSTNELKLSMSIGYAIFDEKVDVSLFDTYRRADSQMYDNKK
                     KINK"
BASE COUNT        18397 a         4947 c         8090 g        15189 t
ORIGIN      
        1 ataggtatat taggaggaat tatatgagtt ataaagtata tattgtggaa gatgatatat
       61 caataagtac attacttaaa gattatattg agaaatatgg atttgaagtg attgttgaag
      121 agaactttga tgatataatg attacatttg aaagttttaa gccacactta gttctattag
      181 atgtaaattt acctaaattt gatggtttct actggtgtag atgtattaga caacagtcga
      241 atttacctat aatttttata tcagctagag atggcaatgt agaccaaatt agagctttag
      301 aaagtggagc agatgattat ataacaaaac ctttttacta tgatgtagta atggcaaaaa
      361 taaagagtca tataagaagg gtatatggtg aatattcacc taaaatagac gaaagaatag
      421 tggagctaga tggattaaag ttttatccag aaaggttaga actagaatta aatggtgaaa
      481 atctaattat aactaaaaaa gaaggtatac tgattgaatg tcttatcaag agatacccaa
      541 aagtagtaag tagagattat cttttagaaa agatatggga tgatattgag tttgtagaag
      601 aaaatacttt aaatgtaaat gttagcagga taaggaaaag atttgaggaa ttaggtatag
      661 ataatgctgt tcagactgtc cgagggttag gatataaatt aaataagaat tggtaaggag
      721 aaagtatgaa gttattttta aaagattata aaggttatat ttttatatat tatataggca
      781 tagttatgac aattatttac tgtaatctta tgaagtttat tagttttgga gagagtttat
      841 atattctttt atttagttct tttatattgt tatgtttttt aatatataag tattataaaa
      901 atagagaagt atataatctg tttgaaaaag gaatacattc tttggaggag tcatttttgt
      961 atttaggaag ctcattcttg gggagaaata tatcagttat tttaaaacaa caacataaat
     1021 tgtatcaatc aatgatacaa gagcataaaa agatacatag tgaacattta actttcatca
     1081 accaatggat tcatcagatg aaaacaccaa tatcagttat aagccttcag cttcaagatt
     1141 atgagggaga agaagtaact gaaaatataa gaaaagaaat agatagatta aataaagggt
     1201 tagatatggc tatgcatttt gcaagggcag ataattttca gaaagacttt atagttgaaa
     1261 aagccaaatt aaatgaaatt gtatctagta ttgtaaatga agaaaaaagg ttatttataa
     1321 aaagtggaat gattcctaag attcaagtag acaaatttac ttatgtttat acagatgtaa
     1381 aatggatgaa atttataata ggtcaattaa ttacaaatgg agtgaaatat tctaaagatt
     1441 atagtaacta cctaactata aaatctgaag aaaataataa atatgtaatt ttaagtataa
     1501 tagatgaagg aattggaatt gtttctaaag atttaaaaag ggtatttgac ccatttttta
     1561 caggagaaaa tggtcgtaag tttggagaat ctacaggtat gggtctttat ctagtaaaaa
     1621 aggtatgtga tgatttaggt cataaagtaa gtataaaatc agaagtagga aaaggtacag
     1681 aagtttcaat ctactttaat aaagatgtaa atagtttgat aaagatgtaa attatctgtt
     1741 acaatagggt ttttagttaa ttgtactttt ttattataca atttattaaa tgaaatattc
     1801 atagttaaat aatcaaaatt taagagagtt tatttgcaaa ccttacatag atgtaaggtt
     1861 tgtgtaatgt ttgcagatag ttaaattttc taatctaaat tacaattaat ataagaaata
     1921 acatgagaga aataacataa aaaaagaaat tttgtaaaaa aggaaggatg tttattgatg
     1981 agtttagatg ttatttttat attgaaatca gtcataatag ctatagtaga ggggtttaca
     2041 gaatttattc cagtttcatc tacaggtcat atgattttgg ttggtaattt aatagatttt
     2101 aaggggcaat ttgctgaaat gtttgaggtt gtaatacaac ttggggctat attagcagta
     2161 gtagtacttt attggaaaaa gataaaagat agcgttatag aattttttaa atttatattt
     2221 actggcggaa aagaaggaaa gattggtttt agatttggaa tgaatgtaat tataggttgt
     2281 ataccatttg cgattatagg agttctattt tatgataata taaaatcatt gtttaattta
     2341 caatctgtaa ttataggatt tatagtgggt gggattttat tattagtagt agaaactttg
     2401 tttagaaaaa agaatcattc aacagataat atagataaaa ttactccaat acaagcttta
     2461 aaggttggga cattacaagt tttgtctgca tggccaggta tgtcaaggag tgcatctact
     2521 attatgggtg gatggatagc aggacttaat tctcctacag cagctgagtt ttctttcttt
     2581 ttagcagtac cagctatggt ggcttcttca ggaaaagatt tatttgaatt tgattattca
     2641 ataatgacac caacattgtg gattgctttg gtagttgggt ttatagtagc ttttattgta
     2701 tcaataatag ttatggaaaa attcgtgaat tttttaaaga aaaaaccaat gagagtgttt
     2761 gctgtataca gaataatcat gggtgtagta cttgcagtat tagcttttac taacataatt
     2821 agtgtataga aaaaatagat ttattatcta taaaaattaa actaagataa attataatta
     2881 acaattatat atatgttatt tcaaaaatta aattaggaag gatagttaaa taatgaaaat
     2941 tttaactgta aataatttaa gtaaagtata tggtaaaaaa ataattttca acgcattaaa
     3001 tgacataaat tttagtatag aagatggaga atttgtagga attatgggtc catctggtag
     3061 tggaaaaact actcttttaa atatgatttc tactattgac aagccaacta caggaactat
     3121 ggaattaaag ggaaagaatc ctttattgtt aagaggtgag gaacttgcat tatttagaag
     3181 aagagagtta ggttttgtat ttcaggattt taatttattg gatacattaa ctataggaga
     3241 aaatatagta ttgccattga cattagataa ggtcagtgta aaagagcaag atgagagatt
     3301 gaatgaagta tctacaatat taggtataaa agatttatta ggaaaaagaa cttttgaagt
     3361 atcaggtggt caagctcaga ggactgctat tgctagagca ttgattaaca atccatcaat
     3421 acttttagca gatgaaccaa caggtaattt agattcaaag tcatcaaagg ttgtaatgga
     3481 gctatttcaa aagataaata aagaaaataa agttactact atgatggtaa ctcatgaccc
     3541 attagcagcg agctactgta gtagaatctt attcataaaa gatgggtcta tatataatga
     3601 aatatataag ggtagtagta gagagcagtt ttatcaagag ataatggatg tacttacatt
     3661 attgggaggc gataactaat gaatataaaa cagtttgcta ttaacaatat ttcaagaaat
     3721 attaaagcgt atttaggata cttagcaagt attgttatat catcttcact gctattttct
     3781 tttattatgt ttacaagtca tccagattta gatgtatctc aatttcctga ttatttaaaa
     3841 gaaggtctca agatgagtaa gataattgca tatctattct tattcttctt tgtgttttat
     3901 tcagttagtg tgtttcttaa aagtagatat aaagaatttg gaatacttta tattacaggt
     3961 atatcaaaac gccaagtaat gaaattaatc tttatagaaa atatgataat aaatatagta
     4021 tcttctgtaa ttggaataat aataggctta atctttgcaa agatattttt agtggttatg
     4081 tctacttttt tagaattatc gccattaaaa ttctatatac catttaatgc gatattgtct
     4141 actttaatct attttatgat tttagctgta ttaacatcaa tatttactta ttttatagtt
     4201 aaagaaaatc aggtgttgaa gttacttaaa ggtacaaaaa ctccaaaacc agagcctaaa
     4261 acatcactaa tgcttgctat attaagtatt tgtttgttta taatagcgta ttataatgct
     4321 attacctcaa ccgaccaata tacaacatat ctgcgcttag ttccagttac atcccttact
     4381 atagtagcaa cttacttgtt cttttctcaa tttagtgtat tttttatgaa gaagctaaaa
     4441 ctaaacaaga gattttatag aaaaaataca aatatgcttt ggatttcgaa cttgatttat
     4501 aaggtaaagg ataatgctag aatatttttc ttgataacta taacttctgc agtagcattt
     4561 actgctatag gtacagttta ttccttttgg aaagatgtag agcgtcaaat taatttaata
     4621 tatcctaaca caatatatta ttcaacaatg acattgcata atgaccctaa aaagcctgat
     4681 agcaaagaaa aagacaagga aagaatgatg tttatagaaa ataatctaaa aaaagagaat
     4741 gtagactata caaggactaa tggtgaattt aaaactgtat ttcccaagaa aagtgatttt
     4801 aatgtcagaa ttatgaaaga atctaagtac ttagaaatta cagaaactat aggggttaag
     4861 cctataagtt ttgaagataa tgaatgcata tcattattat catcaagttt accaggaaat
     4921 aataacgtca aagaaaatat tattgtaggg aatagaggtt taaaggtggc aaaacaagtg
     4981 gaagaatgtg ttatgccagc atattacaaa aatatgtatg tggtaaagga taatttttat
     5041 gatagtatag aatcaaaatt tatagtggat agatttactg catttgaagt agaggattca
     5101 agtaaaacta tagagatatg cagagaattt gaaaataaat ttaataatga gtcagggatg
     5161 caaccatatt tgtttttctc taaagaatta atgataggaa ctgggaaatt aatatattct
     5221 acatttatat ttttagcaat atttataggt ttaattttct ttgttactac aagtagtttt
     5281 ttatataata aattttatat ggattgccaa gttgataaga aaaagtatga gcaattaaat
     5341 aaattgggga tgacatataa tgagattaaa aaagcttcaa caattgagat tggaatagta
     5401 tttttgtttc cttatgtagt ggctgttata cattcaatat ttgcccttac tgcactgaaa
     5461 aattcttttg atatagaagt agcatcatca gctgttttgg taatgggaag cttgttcttg
     5521 gttcaaatag tttatttttt aattgtaaga aataattatt taaaagagat aaggttaaat
     5581 ttagtcaatt tttagttact attattaaat gataataaaa agggtttaaa attaattaat
     5641 attactggaa ttatagatgt ttattttttc agtaatattt tttattgtat taactattaa
     5701 aattttgtac aaatataaaa tataatatgg taaatgtcaa aattataaaa aaattctact
     5761 tatataaaat ataaagaggt tatatgtaca tatgtgtaga aataaataat aaaaatatca
     5821 attaatgtta ttaggggggg ttactcatta gtaaattagc taatacactt agaatgatga
     5881 tattaattca aagcagaggc aaaatgaaat gcaaagaatt atcagaagag ttggaagtaa
     5941 gtgaaaggca aataaaaagt tacaagatgt atttagagca agctggtata tttataaatt
     6001 caacacctgg gatttatggt ggttatgaaa tagataaatg taatagcata tcattaatta
     6061 aattattaga tagtgaagtt tcaattctag atatgataaa ttcacagtta gagtataata
     6121 atgatattta caaaaatgaa tttaataata ttgtagaaaa aataaaagca gtattaaata
     6181 caggagaaaa aagcgataca tatatggatt attttacagt tcaagcacaa cgtaattgtg
     6241 attataagtc tgaaaaaaat aagtgtaatg aaataattag agcatatacc acaaaacaca
     6301 aattttggat agaatattat tcattaaatt caggaaatag tgaaagaatt gttcatccat
     6361 atggtttatt taattataaa tcagatactt atatggtagc tttttgtgaa aaaagattta
     6421 agtttataga ctttaaatta tgtagaatta aagattataa ggttttagaa gaaaaatata
     6481 atgtagataa aagttttagt tgggatgaat atagtaaaaa tagtatagga atttataaag
     6541 gagaagaaat taatgttgtt ataaaaatta gtcatccatt ttcaataatt ataaaggaaa
     6601 aagtttgggt taataatcaa caaatcatag aatatgatga taaatctata atgtttaaag
     6661 ctaaaatgag aggttatgag gagataaaaa gttggatttt aagtatggga gcttatgttg
     6721 aggttgtaga accagataga cttagaaatg atatattgtc agagattgaa aaaatgaaaa
     6781 aaatttactg attttttcaa ataatttata cttggaccaa tatacttcac ggtctagtgt
     6841 taatatatta attgagagag gtgatattat gaaaatgaaa attgaattta gtacaggttg
     6901 tataccaata tcttataatt cgctctttat gagtattata aaagaagcaa taagaaaatc
     6961 taatgaagat tactataaaa acatgtacta ttataaagaa aagaataata aaaaaactaa
     7021 aaattttaca ttttcggttt atataaaaaa gtatagtatt gaaggtgaca attttattat
     7081 agaagataaa gtaatattaa atataagtac tccagattta gaattgggtt ttcatatata
     7141 caatgggtta atgacttcta aaaaatgttt atataaagcc tatgaactta caagaattag
     7201 aatcgattta tcaagagaaa agaaggtaac agaagaaagg gtactattta atgcattgtc
     7261 tccaatctgt gtaaagtcta aagaaggaaa attcttggag ataactgatg atagatacat
     7321 agaagaacta aactatataa ctaatgaagt ggttaaaaat tataggggaa atggattaaa
     7381 aagaaagtta gaatttgaaa atataggtct caaaaagata gtagttaaag agagcttaag
     7441 agaatttaaa aaaataacag gtaaagagta tcaatatatt aatggttaca aaggtaaatt
     7501 tatattgaaa ggtgatattg atgatttaaa tcttatctac aatctaggaa ttggttttag
     7561 acgtgctcag ggatttggag atgtagatat tttagaatgg aggtgagata aatgaagtta
     7621 agagtttata gaggagattg gttttttaat atgggtatag ttggattttt aaatataata
     7681 aagaaagcag ataaacaagc tgaaatattt attatggaag attatatcga atttgatagc
     7741 ttatttcttg aaaactttca tgagtattat tttaattact ttatggatga atatgatgta
     7801 tcaaaaagaa ttaaaaaaaa tattgattac agtataaatt ttattaaatc taagcctgat
     7861 agaatcaaag atggaattaa gaaaattaaa gatagtgtga aacaacaaaa tgataagatt
     7921 aaaaaatttg atgaagaaaa atttaacctg ataaaagaga agctagatag catgtctaag
     7981 ataaaaagtt atgatgaatt tgacagttta gaagctttag tagatgagac tatagatata
     8041 tttaaaataa aatcaataaa tgacaggctt acagcaaatc tttataaata tgtagttcaa
     8101 gataattatt ttggacaagt tagttttttt aatgttgcta aggcgaaatt agatttagat
     8161 ggattaaaac agataatgtt taatgattat ttaagacaaa ttatatattt tggagagtta
     8221 gcagatttat tagaagagaa tgactatgat aagttgaaaa actatttaaa tgatagatta
     8281 aatagtatag ctaaggatat aaatgaaaaa aaagttagta agtctagtat aaatactata
     8341 gaaaaaatta tgaaggaaat aaataaaaac tttataaaga aaaataaaag catagaagat
     8401 ataaaagagt atatggatag tttagaagta tgtgaaatgt gtggtttata taagggaata
     8461 ttagatgaat actcagaaag taattttgct ccacttggag taagcaccaa taatgctagg
     8521 aatatgtttt ggaatcaggc gtatgtacca tcaatatgtg atatttgtaa gctaatattg
     8581 ttctgtactc cagcaggggc tacttataca agaaagaatt atttaattaa tgaagaaaat
     8641 gagttttatt tatttgttaa catggatact tctataaatg agctttttga aagaaataat
     8701 agtttaaaag ctcaaaaaag tgaatattca gattccaaag atgaaaatcc ttttaatcag
     8761 cttataaaaa gtatagtaga agaaaataca ttaaaaagtg aatggcaact tcgtaatata
     8821 ctttttgtgg aatttaaagc aagtatagat tctaaaaaat gtaaaataaa ttattttaat
     8881 attccaactt atttagcaaa attttttaca gacaagtatg caaataaaaa aatacaaagt
     8941 atctatgatt acaagcttaa atcaaatgta ctagatttac tcttgaaaaa tagggacttg
     9001 aagcatctaa ctaataacat tttaagaaat aaagttaaaa atgatatgga aagcaacaac
     9061 aaatcaaata tatcaggaat agattgtttt agagtagtcc aattaagagc attaataaat
     9121 agctacaaaa agggggtata taaaatggat agtaaaaatt tggagaagaa tgatgaaaag
     9181 ctgagaataa tttattattt aggttgtgat atacatgact attttgttaa taaaaattct
     9241 aaaaataaaa tagatggagt aagctataaa cttttaaatt cagttaaagt cggaaataaa
     9301 agtgatttta tggatacaat tataagagtt tttatgtcag ctgaaaaaca gattccagca
     9361 tttattttag atatagaaat tgaaaaagat ttagactttg aatctatagg tcatgctttt
     9421 atatctggat taatttctgg aaaatatgaa ggaaaagaca aactaccaaa cgaaaaggag
     9481 gaaaaataag atgaaaagag ggttaacatt tacagtaata gcacaggctc aaagtttaaa
     9541 ctatggggaa gggataggta atatatcaga attaaaaaaa ttaagtagag atgatggaaa
     9601 tatatataca tttgcatctc gccagtgttt aagatatgac atagtacgat tagcagcaga
     9661 attgtttaat tggaatttac aggttgtcga taaagaaaaa ggtactattc aatttaaaga
     9721 caatataagt ataagagaat cagaagaaat ggatttattt ggatatatga aaacaaataa
     9781 aaaagatgag aaagataaaa aaggaggttc tttgactaga gaagctactg taagattaag
     9841 taatgcaatt tcccttgagc catatagaag tgacatggac tttttaaata ataaaggatt
     9901 agcagataga ataggagaac atcctaatct tgctaacata gagcaacatt taagttatta
     9961 tacatataca gtgactatag atttatctaa aataggtaag gatggagata tagaacttga
    10021 taataaagag aaatgtagaa gagtagtaga atttttagaa ataataaaag tattgaatag
    10081 aaatattaga ggtagacaag aaaatttatc tcctctattt gtagtaggag gagtatatga
    10141 aatagcaaat ccatttttct tgggtagaat taaattaaaa ggagataaaa atggatttaa
    10201 aataaataag caagctatag aagaagtaat tcaaggtact tttttaggaa aagatttaaa
    10261 agaatttacc tatgttggaa tggtagatgg ggtatttata aataaagaag aatttaaggg
    10321 attgtttgaa gataattttt taagtgtaga taaatttttt ggacaactag ttaaagaagt
    10381 aaaggaatat tatggggtga attaatatga aggttttgaa attagattta tttcaagaaa
    10441 ctgcatgcta taaaaaacca tttgctatga agattacaga gacatatcca ctgcctccat
    10501 attctacaat aaatggatta attcacaaaa tattaaatgc tacagaatat ataccattta
    10561 atataagtgt acagggaaca tatgaaagta tatttaataa ttaccagaca acatattttt
    10621 ataaaaaaga tagtattaca tctatgccaa tgaatagtca tatgctattg aatgtaaatt
    10681 tgataattca tataggtggg gattttgatt tattggaaga aatatatgat agtttattaa
    10741 attatgacga gcacttatca ttaggtagaa aagaagattt agtaagaatt aatgatatta
    10801 gatttgtaga agtcaataaa tttgaagtag atgaggatat acaattagat gattatgaaa
    10861 attataagtt atcagaatgt aatataagaa tgcctatata tataccaaaa acaagtttac
    10921 aagatacaga tatagatggt ataagttaca gactcaataa ttactatgat aataatgctg
    10981 tggaagataa aaaaagggtt tggaataagg tagatagtta ttatgttgag gatggaaata
    11041 ctatatcaat tggagatata ttattagata atgaaggaga tttattatat tttaataata
    11101 taaattagga gggagagtat gttatatgct aaatctaatc cagtggaaac acttagagaa
    11161 catactgatg aacttttaaa acaaatgaat gtattaagag aaagttatgg aaaaaatata
    11221 aatagcttag attttctaga ggaagagata ttttggcaat tacttgactt tgttatagaa
    11281 tttcatgata ttggaaaagc attttcaccg tttcaagagt taataaaaag cagaatggat
    11341 actaaaataa atgaacctaa aatagtaact catttagaaa ataatgtagg ccataattat
    11401 ctttctcctg catttattga ttatagttat atagacagga aaaaaaataa agaacttaga
    11461 gcagtattaa atcaagttat tgtatatcat catgaaagag atatctttat agataaagat
    11521 tttaaaaatt taatacaaaa aatattagat gaagatttaa taaataaagt ttatgaacta
    11581 cagcgtgaat ttaaagttag atatccaata aaaacagaaa aattgagtaa agtatatcta
    11641 caaagtgtag aaaaaagaat agataaaaat cacaagtact ataatttata tattatgtta
    11701 aaaggaatac tacatagact agaccatagt gcttctgctc atgaagttgt ggaatgcaat
    11761 aatgttataa atataggaga acagacagaa aattatttaa tgaaagagtt tggtagtttg
    11821 agagaggctc aatcatttgc taaatctaat agaaataaga atataatact gattgcatct
    11881 acaggaatgg gaaaaacaga aactgctttg atttggatag ataaggataa agcatttttt
    11941 acactaccat taagagtaag tataaatgca ttatttgata gggctaaaaa tataattgga
    12001 gtaggagaag caaatgatac attcttaggt ctgttacatt caacagcaat agattattta
    12061 gaagaaagta atcaggaaaa ttcaagtgag attgtagatt tagcaaagtt attatcttgt
    12121 aaactgacat tttccacaat agaccaaata tttaaatttc cttttttgta tagaggctat
    12181 gaaaaagtgt actcaacatt agcatattca aaagtagtaa tagatgagat acaagcatat
    12241 tcaccagaaa tagcagctgt attggtaaaa ggtatagaaa tgatacataa gataggtggc
    12301 agatttatga taatgacagc tactatgcca accatatata tagatgaatt aaaaaaaaga
    12361 ggtgtaatga atagtaactt agctgacttg acatgtaaca cagaaaaaat aagacattgt
    12421 gtgtctattg tagaaaattc aatagatgaa aacttaggta aaataataca aagtggaatg
    12481 aataaaaaag ttcttgtaat agtaaataca gttaaaagtg ctgtagaaaa atatgagtta
    12541 atagaagaaa tagttaaacc aaaaggaata gatgtcaact taaatctttt acattctatg
    12601 tatatacaag aagatagagc aaaattagaa aaatatataa aagaatttgc agatagtgat
    12661 agtaatggta tttggattac tacacaatta gtggaagcat cccttgatat tgattttgat
    12721 gagcttcata cagaaaattc aacacttgac agtctatttc aacgatttgg tagatgttat
    12781 agaagtcgtg agtatgaaga aaatagccca aatatattta tatatacaga aaaagctaca
    12841 ggaataggta gtatttatga taaggatatt gtagaaaaag gacttgagct actgaaaact
    12901 tttataaatg gaaaagaatg tgtgaagatg aaagaaaaat ataaggtaga aatggttaag
    12961 atactatact ctaaagaaag tctagaaggt acagcttttg aaaagagatt taccagtgct
    13021 ttaaatattt tagatactat aacgccatat agtttaggtt ctaaagatgc acaaaatata
    13081 cttagaaata ttgaaggata tacagtaatt ccagaggata tatataataa aattgaagaa
    13141 actttaatca aagattatga agagttggga caagagctta taaatgcata tagtaatgat
    13201 gatagacaat tgataaataa aataaagtct aagagaaaaa aagcaagaag agagataatt
    13261 aaaaaaactg taaatttacc aatatataaa gctaaacaaa atgtaattga catacaggtg
    13321 aaaggtcttg aagatttaaa aatactttta tataaatatg atatacacga aaataagcat
    13381 acaaaacaga tatttggtaa aggtgtgctt ataagtaatg aaatagacca atttatttga
    13441 tttttatatt aacaatgtgg ctaaaattta ctaaagaagg tgattttgtg ccaatagatt
    13501 tagaagtatt aaaagttcaa ggcgtaaaaa taaattatta ttatgtatgt aaaagaaaac
    13561 tatggctatt ttctaaagga ataactatgg aacaaaatag tagtagagta ttgtctggaa
    13621 agatagtaca tgaagattca tataaaagaa tgaaaaataa agaaatgttg attgatgata
    13681 tcttgaagct agatataata aaggacgagt atgtaaaaga agtaaaaata agttcaaaga
    13741 tgaaagaagc agatgaaatg cagttatact attacttata ctatttaaag aatagtatgg
    13801 gtatagagaa aaaaggaact ataaattatg ttaaggaaaa gaaacaagaa gaattgatat
    13861 tgacagaaga aaaagaaaaa attattgaaa acacacttat aaatataaat gaaataaata
    13921 tgcagttatc tcctcccaaa attgagaaaa aacattattg tacaaaatgt gcttattatg
    13981 aattttgttt tgtgaaggag gagtaacaat gtataaagac tattatatta taagtaatgg
    14041 aaatataaaa aggaaagaaa atacgattta ctattacaat ttagaaggtg agaaaaaaat
    14101 attaccagta gaacaagttg atgatattca tatatatgga gaagtagatt taaacacaaa
    14161 acttataaat tacataagta aatatggggt gatgcttcat ttctacaatt actatggata
    14221 ttattcaggt acatattatc caaggaagaa aaatgtatca ggattcagtt tagtatgtca
    14281 atcagcctgc tatttagata aaaatgaaag attatactta gcaaaatcct ttgtggaaag
    14341 tgcatcatac catattttaa gaaatatgag atattacaaa gttgatgaaa gtttaattga
    14401 tactataaag gatgaactaa atgatttgaa tgatgtcaaa gaaatatcag aacttatggg
    14461 taaagaaggc agaataagaa aaacatacta taaatcattt aatcaaatat taaaagatga
    14521 ttttgaattt tataaaagag aaaaaagacc tcccaaagac ccagtaaata ctcttatgtc
    14581 ttttggaaat agcataatgt atacgaatgt tttaagtgag atatataaga cacaacttga
    14641 ccctacaata agctttttac atgaaccatc aacaaagaga ttttctttaa gtttagatat
    14701 tgcagaaatt tttaagccaa ttatagttga cacaataatt tttagcctga ttaataataa
    14761 aagaattaat aagaatgatt ttgaatatca agacaaaatt tgttatttaa gtgaaaaagg
    14821 aaagaagaaa tttttgcaag aatttgaaaa taagatgcag acaactataa aacatagaaa
    14881 tctaaataga aaagtttcat atagaatgtt tataaggtta gagtgttata aattaattaa
    14941 acattttata aatgatgaaa ggtacaaagt tttaaaggtg tggtggtaag tatgtttgtt
    15001 attgttactt atgatattgt tgaagcaagg tcgttaaata gaattagaag gatacttaga
    15061 aaatatttga cttggacgca aaattctgtt tttgaaggca atattactga tggaaagtta
    15121 cataaatgta tttctgaaat agaaaatatt attgataata gcgaggattc aatctatgtt
    15181 tatgagataa aaaatcctaa ttcaattaaa aagaaatgtt atgggattga taagtattct
    15241 gatgaaatgt ttatataggt ttgcagtgag cgatatttat gctaaaatag gtgttaacag
    15301 ttggaatata agggattgaa ggtgtatgat aactgttatc aattgcacta ctgctcgctc
    15361 actgcaaatt ttgatgtttt tattgaatta taattgcttg attgaagtat tttcaatgta
    15421 ttcaattata cctattttgg ggttttatat taactaagtg gtatgtaaat aagcaactat
    15481 agtagaagca aaagcgacaa caactaaagg ttttatatta actaagtggt atgtaaattt
    15541 ggttaactta agtgctatga aaggaattaa tgaagtttta tattaactaa gtggtatgta
    15601 aatttaaagg tgcaagtctt aatacttgtt acatgagttg ttttatatta actaagtggt
    15661 atgtaaataa caaagaacca tttgaatcac gaaaactaaa aaacagtttt atattaacta
    15721 agtggtatgt aaatttatta accttatcag aaacctgacc atcccaatta tagttttata
    15781 ttaactaagt ggtatgtaaa ttccatttgt ctaatgcttc taactctttt tttatacgtt
    15841 ttatattaac taagtggtat gtaaattcca aaatgggctt ggagttaaga gttgtataag
    15901 aaacgtttta tattaactaa gtggtatgta aattaattac acactccctt ttaacgaaca
    15961 tattttagat tgttttatat taactaagtg gtatgtaaat agactcgaca tagaatcggt
    16021 tactaaatcc gagggtttca ggttttatat taactaagtg gtatgtaaat actaaacctt
    16081 tttgtttact tttatttctc attttacgtt ttatattaac taagtggtat gtaaatccgg
    16141 atttttctgc ttcgactgtt gttattgaga cttgttttat attaactaag tggtatgtaa
    16201 atagtaattc catgcaatct tttatattgc tttttaacct gttttatatt aactaagtgg
    16261 tatgtaaata gatatacaag gtgaaagtgt tgaaatttca atcttgtttt atattaacta
    16321 agtggtatgt aaatattctt agatgttata atttgtgttg catttaaatc gttttatatt
    16381 aactaagtgg tatgtaaata caatattatt gttcatttca tttaataatt ctagtagttt
    16441 tatattaact aagtggtatg taaatttgtt tataccttta atctttaaaa ttaaatataa
    16501 aagttttata ttaactaagt ggtatgtaaa tcattgtata ctaagcttga tatagtgcaa
    16561 aatgaaacgt tttatattaa ctaagtggta tgtaaatact aattgggtta aacaaatgga
    16621 tataactaaa ttagttttat attaactaag tggtatgtaa atccaaccct tcgcacgaat
    16681 ccgtgcgacg aaatataaag ttttatatta actaagtggt atgtaaattg taaaaatatt
    16741 gcttagcata ttgatatcac taactgtttt atattaacta agtggtatgt aaatacaaga
    16801 ggtttttcag caatatatgt acttctataa tgttttatat taactaagtg gtatgtaaat
    16861 tcatcaatat taattttggg aatatcaaca ttttttgcgt tttatattaa ctaagtggta
    16921 tgtaaatgcg ataagagatt cgagtctttt tactgggctt aatcgtttta tattaactaa
    16981 gtggtatgta aataggataa agaaaagact cacacaagac acagtgtcag gttttatatc
    17041 aactaagtgg tagataagta gaatattagc aatatcaacg agtatttaga aacttatttt
    17101 atattaataa agcagtatat aaaattgtag aataaacaat agcatatact aaaacatcca
    17161 ccttatatta actaaatcat atctaaataa aatgcactta cctataaaca ttataaaatc
    17221 aatacaaaaa tgaggtgaaa caaaatttat gataaagaaa ttaaacaata aagacataaa
    17281 taaaatcatg gaaatatggg aaaaaagtac aatcaaagca catgacttta taagtaaaga
    17341 atactggcaa aataactaca atactgttaa aaacgaatat atacctatat cagatacatt
    17401 tgtatatgat gatggagatg aaataaaagg atttataagc ataatagata aaagctttat
    17461 aggagcttta tttatagaac ccaaatacca aaatctaggt atcggaggta aacttttaga
    17521 ttatgcaact aaaaaatata aaagtctaag tttagcagta tataaagata ataaaaaagc
    17581 agttgtgttt tataataaaa aaggttttaa tatagtaaaa gaacaagtaa atgaagattc
    17641 aggatttaaa gagtacataa tggaatatag taaataatat gattacattc tattaaaaga
    17701 ataattaatt tattttctat ttgattttta taagagtaat ttttaaaata gactatatga
    17761 agataaagat atttattata gatgtaagtt aatcagaatt gatatagcta caaaagagga
    17821 aaataaaatt gtatggtgta tttcagattt aacaaattta ctatttaaag ataataagtt
    17881 tgatataata ttaaagatac tttcacctat aaactatagg gtatttgaaa gaattttgaa
    17941 taattatgga atgctaataa aggtttgtta aaatttaata attattagat tatagaaaat
    18001 tgccatgaaa ttagttcagc aagaactagt ttcatagcaa ttttaattct tataagtgta
    18061 tttattttta cagatttaaa aaacttatac agtatctaat aatgtaatct tattacttta
    18121 gcctataaat atttaaatct taaaatgttc tctttgagat aagtttattt cagagtcaca
    18181 atttccacaa cagtaagatg ttgagacatc tttaaatttt tcagtattct catcaaagtt
    18241 catttttttc tttgcaataa ttcctacctt agttaaatta gaaccacaat tattacagtt
    18301 ttgaatttta cataggttat atagtttatt aagtgtaata tcttctaata attcatcaca
    18361 attacaacat ctgatagaag aaacttcact agacatttca ataggttcag cttctttaga
    18421 agaaaattta tatgttatgt catttgatac tttataacct gtatcaaata tattagaatt
    18481 acaacttgga cataagtact ttggaagata tatatctttt ttttcaaaag tatcactact
    18541 aatattacag tttaattcat ctaaaattgg tctttcatct aggttattac ctttatttaa
    18601 gcttagaaga taattataaa tttttttatc ttcattctgt tttgatgtat ctaacaaatg
    18661 ctccattata ttagtgtatt ttttgttttt tgaaggcatg tttaaatcca gataattgtt
    18721 ttcaatagca aagcatacta tattgaaata gttatattta actgcaaaaa ataataacat
    18781 ttcatcaaat actttatcta ttattttgtc agaaatactt atccaaaaat ctggtgactc
    18841 taagtaaact ttcctactaa gttcatctaa ctcttcatct atattattta tattattttc
    18901 attaactttc atatcatcac ttccttttaa atatcttgtg tataaactat ttgttactat
    18961 tttttatata aaataaaatc ttatataaaa atatatcata ttataattta caagtatagt
    19021 ctaaatagag ctgtttttaa gcaaagtttt ataaatgact attaaaatat aaagtaacag
    19081 tataaagttt acttataaat taaacgtaaa cctaaatact tgtaatggtt gacaaaaaaa
    19141 cgtctttatt ttatactaaa acaaagcttt aatagaggct ttgtatatgt acatataaac
    19201 taaatgaaat aagacatatg aagggaatta taaaaatgaa aaatactaaa gtaaatattc
    19261 aagatttaac taaaatgtca atatgtgtag cattattgtg tatatcatct tacatatata
    19321 taccattgcc atttactcca gcaggagtaa cagcacaaac aataatgata aatttgatag
    19381 ctttgatttt aacaccaaga caagcattta gtacagttgg tgtatatata atgataggtt
    19441 taattgggat tccagtattt ggtggaggta catctggtat tggaaagttg ttaagcccaa
    19501 caggtggttt ttattttggg tttttgtttg cagcactaat tattagttta ttaaaaggac
    19561 gaaataatga tattaagaga tatatactaa taacaatttt tgttggaatg cctattatat
    19621 attttatggg aacagtattt atgtgctatt ttaatcaaat gacagtagaa gcagcacttt
    19681 ttgcagcagt agtaccattt ttaattggag atacaataaa gtcggttatt gcatcttttt
    19741 taggcactaa actgaacaat gtactcagaa gaaatctatg ataaatctat aatataagga
    19801 agggtgtaat gtatgaagga agtttttata ttaggaggat tgagaagtca tataggtcta
    19861 aaaaatggaa tttttcaatt tgttcaacct gaattactag gagcaagtgt tttaaaagat
    19921 ttaattaaaa agtatgaaat tgacaaaatt gatgagatta tatgtggaaa tgcagttggg
    19981 acaggaggga atatagccag attaatgact ttaacagcag gagtgtcaaa tgaagtacct
    20041 gcttttactg tggatatgca gtgtgcttct gctatgatga gtatagacat agctttttca
    20101 aaggttaaat ctggtcagtg taatttaatt attgcaggtg gttttgaaag tagttctcta
    20161 caaccaatgc gcacatatca taagaatgat aagcgatata atacaaataa tccaaattat
    20221 acagttgctc aattttctcc tgatgataat agtcaaaata gtatgctcga aggggcggaa
    20281 agagtagcag agttatatca aattgagaga gcagacttgg atttttgggt aaaggaaagt
    20341 cataaaagag caaaagaagc aagagaagaa aaaatacttg aggatattat atctcctata
    20401 aataatagta cctatgatga aggtatacgt gataagatga gtcaaaggtt attagatagg
    20461 atgccaccaa ttttaggaaa agaaactatt acaaatgcag caaattcatg tcttattaat
    20521 gatggagctt catttattat aatatgttct aagaagtatt tagaacatgt aaaaaagaag
    20581 cctaaaacaa aaattattaa tacatgcaca ataggaacag atgcaacctt aagtccaaca
    20641 tcagcgatac aagcaatgga taaactttta gagatagaaa gccttaatta tttagatgta
    20701 tctgctgttg aatttaatga agcatttgca gttatagatg tgttgtttca aagaaagtat
    20761 ccagagttaa tagagagata taatatattt ggtggagctt tagcgtatgg acatccatat
    20821 ggtgcttcag gtgcaattat tacattacat cttttaaaag cattagaaaa aactaagggt
    20881 agatatggaa tttgctctat agctgcagct ggtggtctag gttcagcttt actgcttgaa
    20941 agggtgtaga ttatgaacta ttatgaactt ttaagaataa aaaaagagag gcataaaaat
    21001 aaaaattttt taattataga tggagaaaaa tatacttatg aaaatatatt aagttatagt
    21061 gagcaactag gaaaacaaat ttctttaggt gaaaatatct caattttaat atattctaaa
    21121 aatataatgt ttcagttgat tagttttttt gctattaatt attcaaaaaa tattcctatt
    21181 atttgtcatt ataatctttc taaaaaagta ttagatgata ttttattaaa aaataatatt
    21241 tcaatcatta tatctgatga atctatggac gttattgatt tatatgatga taacaataat
    21301 gaagtagcaa aaaaagttaa tttttctatt tttaatccta aatttaatct atatatgtat
    21361 caatataaga accttataaa ttatttagat aaaagtattt gtatgggagc tttatcttca
    21421 ggttctacaa gtgttccaaa ggtattatat agaacatatg agagttgggc tggatttttt
    21481 cctatacaaa atggtatttt taatatctct ggggaatcaa tcttgtttgt taatggaaca
    21541 cttagtttta cagggaatct aaattctata atgtcagtat tatatgaagg aggtagtatt
    21601 gtaataagtt caggtcttaa ttgtaaatca tggataagga caataaaaca atataatgta
    21661 actaacatat accttatacc tacaaaactt caacttttgg ttaaacactt aaaagaacca
    21721 gtactcaaag tagtaagtat ttttacaggg tctcaattat tatttgaata tactgcaaga
    21781 aatctaaaaa aatatatgcc aaagtctgaa attattttat actatggggc aagtgaatta
    21841 aattatatta catatttatg ctatgatgaa ctaattgaaa aacctctaag tgtaggcaga
    21901 ccatttccag gaatagatat atatattaga ggaggaaaaa tttttgtgaa tacagaatat
    21961 gcagtatata atgcaaccaa accttattct gtcaatgata ttggatatta tgataatgat
    22021 ggatacttaa tatttgaagg aagaagtgat gacattgtaa atattggagg atttaaagtt
    22081 agttctgcta agattgaaaa tgaagttaaa aaaattcctc aaattgaaga tgcaattgta
    22141 ttgccgtact cagacactat aagaggaaat caaattgtat tatttgtaat aactatagac
    22201 aaaattacga agaaagattt gttgattaaa atgaaagata atctaatgaa aaatgaaatc
    22261 ccaaagaaaa ttatcttttt aaattcattt ccatatacct cttctgaaaa gatagataga
    22321 ttagcattat taaaaatgtt ataagtataa tatttagtaa ttttagtgaa ccaatgtatt
    22381 gataaaaaca tattgtatat agtaaaggag gcttacaata tgtcaaatca atacgatata
    22441 actgttattg ggggagacct gcgacaagtg tacatggtaa aaaaactaat taataaaggg
    22501 tattctgtag tcacttatgg gatatcaaat gaaattataa atggaatcgt tggaaaatca
    22561 acttcactag ctgaagcact atccaaaagt tccattattt tatgtccaat tccatttaca
    22621 aagaatcaag ttaatattga aaatacggat gattgtcttg atgctactat tgataacttg
    22681 ctaattaatt tatctcctaa tcaaatatta tttggaggta acataccaac taaagtttgt
    22741 aaattttgcg agcaacactc tattcagatt tatgatttta tgaaaatgaa tgatgttgct
    22801 attttaaatg cagttgctac agctgaaggg gctatagcag aagctattaa aagaagtata
    22861 atcaatatac atcaaagtaa ctgtttagta ttaggttatg gaagatgtgc acaaatactt
    22921 gcaaaaaaat tatacgcttt ggatgcaaat gtatgcgttg gtgcaagaaa taatgatgca
    22981 agaagtacgg ctaatgcatt tggatattcc agtatttctt taagtcaact tgatgaaaaa
    23041 cttttacaat ttgattatat ttttaacacc ataccagagt gtattttaac gaaagaaaga
    23101 ttagaaaaag tatcacaaga agtaactatt atagatatag cttcttctcc tggaggagta
    23161 gattattctg ttgttaaaga agtagggatt aatgcgactc tttgccttgg gcttcctggt
    23221 aaatattcac caaagacatc tggtgagatt cttgttaacg ctatagaaaa tattataaat
    23281 gaaagaaggg attagaaatg agacttgaag gacttacaat tggagtagga tttactggtt
    23341 cgttttgtac ttatgataaa atatttatag agttagagaa tttggttaaa gaaggagcta
    23401 atgtacacac tatattttca gatgtatcac aaaatatcga ttgtagattt ggaaattctg
    23461 aagagtttat gaaaaaagca tatgaactta caggaaataa acctatagtt acgattgaag
    23521 aggcagaacc atttggacct aaagggattg ctgatattat aataatagct ccttgtactg
    23581 gaaatactgc tgcaaaatta gcaaatggta ttacagattc accagtatta atggcagcaa
    23641 aaggtcatct tcgtaatgat aaaccactag taatatcaat atcaacgaat gatgctctaa
    23701 gctttaattt caaaaatatt ggtattctcc ttaattctaa aaatatatat tttgtacctt
    23761 ttggtcaaga taattgcaaa gcaaagccta attctatgat tgcacatact gagctaataa
    23821 ttccaactat agaacttgct ctagagaaca aacagctaca gcctgttata aaaagtccac
    23881 atcaatagaa ttggttctta taatattatg tattagatac aatactagca aagaggctgt
    23941 ctcaaaatag agagaagcct ctttttcatt gttgaaatga tgaaaatata tttaatttca
    24001 tacaaaaaaa tgcatcatga actaaaaagt ctattttgag ctaatctccg taatttttgg
    24061 tatattaaaa ttatttactt aatccaattt ttaaattaga atttatatcg ttttgactct
    24121 tcaaatatca atctaactgt tgttgataca aaagaagagg acataattaa aacaaatact
    24181 gatagtcctc taataaacaa attatctaca taataagtta ctattccaat acctccaatc
    24241 gttaaaatta cacttataat caaagaaaaa atgctaattg tttttaattt tttcatgctt
    24301 atcctccttc ttataaactt caaatttatc tatataatat taaatatatt ttatttcata
    24361 gtatataaaa atcatgaata tatatttttt taattatact atatgttgta aatgtttgta
    24421 catttaacta tatttacttt aacttgttaa tcaagttatt attttcattt tcaagtattt
    24481 ttatttttaa acttgataga atatcatatt aaaaattctt tttataaagt tttatttgca
    24541 aaaaatacaa aaatataaac gttaaaaaaa taacgttatc cttaagtttt tagatgattt
    24601 tattagtgat ttgcgtaaaa aataaagtga tatctagtta aattaagtat ttgtaaagct
    24661 agatattatt agtagaaatg atgttatatt agataaaaaa taaaatgtta aaaaaataac
    24721 ttgaaaatag aaaaagtaaa tgctataatg ttaataaatt aacataataa ataaaaaact
    24781 atctcaggag gttgttaaaa tggctcataa agaacaagta ataatagaaa ataaaaaaga
    24841 aaatgaagtt atgtcagttg gtttagatac tgtaaataac gttgaaagct tagttaaaaa
    24901 actagcaaaa atcagagaag ctcaaaaaat atttgctaca tatactcaag agcaggtaga
    24961 taaaattttt ttagcagcat ctttggcagc taataagcaa agagttcctc tagctataat
    25021 ggcacaaaag gaaactggta tgggaattgc tgaagataag gttataaaga atcattatgc
    25081 ctcagagtat atatataatg catataaaga aactaaaact tgtggtgtta tagagaaaga
    25141 cgaagcattt ggtatgacta aaattgctga gccaataggt gttattgcag cagttgtacc
    25201 tacaactaat ccaacttcta ctgctatctt taaagcactt ttagctttaa aaactagaaa
    25261 tggaataatt ttctctccgc atccaagagc aaaaaattct actatagagg ctgcaaaagt
    25321 tgttcttgag gctgctgttt tagctggagc tcctgaaggc ataataggat ggatagatga
    25381 gccatcttta gaattaacta caactgttat gaaagaagtg gatttgaccc ttgctacagg
    25441 aggtcctgga atggttaaat cagcatattc atctggaaaa ccagctattg gagttggtgc
    25501 aggtaatact ccagcaataa ttgatgagag tgcagatata aaaactgctg taaactcaat
    25561 tcttgtatct aagacttttg acaatggtat gatatgtgct tctgaacaat cggttatagt
    25621 attagaaaat atatacaatg aagtcaaaaa agaatttaaa gagcgtggtg cttatttatt
    25681 agataaagat gaaacagaaa aaataagaaa tataatttta gtaaatggag gattaaattc
    25741 taaaatagtt gggcaaacag cttgtactat agcaaaactt gcaggctttg aagtcccagt
    25801 agatactaag gttttaatag gtgaagtaga atcagttgaa atagaagaag cttttgctca
    25861 tgagaaactt tcaccagtac ttgcaatgta caaggccagt gattttgata atgcagttag
    25921 aaaagcagaa aaattagtag aagatggtgg atttgggcat acatcttcat tatatattga
    25981 tgatgtaaat caaagagaaa aacttgataa gtttacttca gccatgaaaa cttgtagaat
    26041 tttaattaat actccttctt ctcaaggtgg tataggggac ttatataatt ttaaactagc
    26101 tccttctctt actttaggat gtgggtcttg gggtggaaat tcagtatctg aaaatgtagg
    26161 agttaagcat ttacttaata ttaaaacagt agctgagagg agagaaaata tgctttggtt
    26221 tagatcacca ggaaaagttt atttcaaaaa aggttgttta ggggtagcat taaaagaact
    26281 taaagatgta atgaataaga agagagcttt tattgtaaca gatacattcc tttacaataa
    26341 tggatatact aaagcagtta ctgatttatt agatgaaatg aatattaagc acactacatt
    26401 ctttgaagta gaaccagacc caactttgga atgtgctaag ataggcgcta aagctatgag
    26461 agaatttaat ccagatgtaa taatatctat aggtggagga agtgcaatgg atgcaggaaa
    26521 gataatgtgg acattgtatg agcatccaga tgtagatttc caagacctag ctatgagatt
    26581 tatggatata agaaaaaggg tatatacatt ccctaaaatg ggtgaaaaag ctaattttgt
    26641 agctatacca acatcagcag gtactggttc agaagtaaca ccatttgctg ttataactga
    26701 ccaagataca ggggtaaaat atccattagc agattatgaa ctgatgccaa atatggctat
    26761 agttgattct gatatgatga tgaatatgcc aaaaagctta acatctgctt ctggtataga
    26821 tgcattaact catgcattag aagcatatgt atctatgctt gcaactgatt atacaaatgg
    26881 attagcttta caagccatta agagtatatt tgaatatctt ccacgtgcat atgataatgg
    26941 tgcaaaagac ccagaagcta gagaaaaaat ggcaaatgct tctacaatgg caggtatggc
    27001 ttttgcaaat gcatttttgg gtgtttgtca ctctatggct cataaattag gtgctttcca
    27061 ccatgtacca catggtgttg caaatgctct tttgataaca gaagttatga aatttaattc
    27121 ttcagatgca ccaaagaaaa tgggagcatt ctctcaatat aaatatccag aagcattaaa
    27181 aagatatgct gaaatagctt catttttagg attaaaggga aattctgatg aagagaaatt
    27241 ccaaagttta ttggtagcta ttgaagattt aaaacttaaa gttggtattc ctaaatctat
    27301 aaaagagttt ggagttgaag aacctaagtt tatggattca atagatgaaa tggtgattca
    27361 agcatttgat gaccaatgta caggagcaaa tccaagatat ccacttatga atgaaataaa
    27421 agatatgtat ttaaattcat attatggaag ataaatttac tataaaatag ataaaataaa
    27481 aactcttata cataaaacaa agttttattg tataagagtt tttttgttta gaaaaattga
    27541 tagtttttat tctttaattt aaaattcaac taaatagtgt tattatgata ataaaagaat
    27601 gtttttatgg ttattaggtt gattttatgt gtaacattta agaatagtta tatacaatat
    27661 aaatgatgaa tcggtataaa aaggatatat tatctatatg aagtattgac taattagggg
    27721 aggagtcgaa tgacagggag aaataaaagt tttacattaa ttacaattct ttttttaaca
    27781 tcattatttt tgacaatggg gtatatctat atagaagata caaaacatct tcttatgtca
    27841 gaggcaaaaa tacatataaa agaagttgca gtacaaggct ctcaactagc acaaagacaa
    27901 attgaaaaag atttagatgt tttaaatatt ttttctaact attatgcttt aaatcaagat
    27961 atacctaatg aggaaaaaat gaaaaatctt ttagatgaaa tggaaaatca aaaattttac
    28021 actatggcaa tagttgatat aaatggtaat gctgaaaata ccaaaggtga taagttttca
    28081 gtgaaagata gagaattttt caaaaactca attaaaggta agaagtatgt ttcaagtcca
    28141 tatgttgatg aagtaaataa gtctataaag aagattacta tttcagtacc attattaaat
    28201 aatgataaag tagttggagt tttatattgt acttacaata tcaatactct gatgaaactt
    28261 attaatgtat ctttttatga aaataatagt atatcttatg ttgtaagaaa tgatggtaca
    28321 attatccttc atccgcaaga ggatagccta tcaaaaaata tttataaatt gttaaaacag
    28381 gataatgata ttcgagaaat aaatagatta aaaaaagaat tgcaagaaaa taaaacaggt
    28441 gcaactgtat taaatatgtt ggaagaaaga agatatttag gatatgctac tatggataat
    28501 gggaatagtg aaaataatta tataaaagat tggaatttga ttttttcaat tccagaaact
    28561 gttgtattta gtaattcaga acaaataatc aacagagcta tatatgcagt gttatttatt
    28621 atattaatat ttattgcaat tattttttat ataatttata taaaaaaatc caatgaaaaa
    28681 gaaatattaa gtttggctta tgaagataaa gttacccata taggaaatca aaataaattt
    28741 tatagagagt gtagtaagta tcttttagat aaaccatctt taaattatat tatagtatat
    28801 tttgatatta ataactttaa gatgattaat gatacttttg gatatgaatt tggtgacaat
    28861 ttattaatta cgatagcaaa agctttaaaa gaagaattaa cagaaggtga agtatatgca
    28921 cgactttcaa gtgattattt tgcaattttc tgtgattata aaaatggaaa aaataaacgt
    28981 ataagaaaaa ttgatagtat acgaaataat attgaaagta atttgagtat agtatttgaa
    29041 atttctctgt gtgtgggtat ttattttgtt gaagaaggtg aagtagatat ccaaaaggct
    29101 gtaaataaag caaatatggc taggtctgtt gctaaaggta aaaatataaa ttatgctatt
    29161 tataatgaag atgttaggaa taagcttagt gaagaatcaa tgattttaga tgatataaaa
    29221 atagcattgg taaaaaatca atttgaagtg tattatcaac ctaagttttc acttgtaact
    29281 ggagaaatga tagggagtga agcactaata agatggaatc atccagaaca tggttttata
    29341 agtcccgcag tatttattcc aatagcagag aaaagtaagt tgattttaaa aattgggaga
    29401 tttgtatttg aaagggtttg caatgattta tctgaatgga aaaagcaagg taaaaagata
    29461 gttccagttt caataaattt atctagagtt gagttgtatc aaccagatat tgtaaaattt
    29521 atcaataaaa caatacaaat gtataatcta agttcagatt taattgaaat agagataaca
    29581 gaaactgtgg ctattaatga gttaaatatt ttaaagaatg ttttaaatga gttgaggaag
    29641 tatggatttt ctatttctat ggatgacttt ggtacaggat attcttctat tagttgttta
    29701 agagatatgc caattgatat attgaaacta gataagtctt ttcttaatgg tattgaacat
    29761 gatgagagaa gtcgcaatat agcaaaatca atagtgtctc tagcaaaatc actagattta
    29821 gttgtaatta tagaaggtgt tgagagtaaa gaacaagctg agttaatgaa acaatttgga
    29881 tgtgatttag ttcaaggatt ttattttgca cgaccaatgc cagctaagaa ttttttagat
    29941 ttactttaaa acaaaattta atttttatat aaagcactat tatgaatgtt tgattttaat
    30001 aaatcactgc taaataatta gtgttttttt attttatcaa tgtattgttt aatataaagc
    30061 aaaaaataat tgtattttat aaaaattata ttaaaaataa atttattttg ctaataaaca
    30121 tgttattaac aaaagaatag tactataatg tatagatgat aaaaacttta ataaaaattt
    30181 agtgtatagg agttgtttaa gtatgataga tgtgagtatt gatgacaaac ttaaggaaag
    30241 atggcctaat gctaagttgg gttgcattca agcaaaagta gcagtaaata aaagctcaga
    30301 aaaattaatt aatgaaataa atgagttttg tgatactata aatcaaagtc taaaagttga
    30361 agacattacg aaactagata aaattaaaga tgcaagaaat gcttataaag agcttggaaa
    30421 aaatccaagt agatatagaa catcttcaga agcattggtt agaagaatta ttcaagggaa
    30481 gggtctttat acaataaata atatagtaga aataaacaat ttaatatcaa taaaatcatt
    30541 atatccagta ggtacatata atgtaagcaa attacattca ccagtatgtt ttactgtagg
    30601 agatgaagga gaacagtata agggtattgg gaaagaatta ataaatatag aaaatcttcc
    30661 tatattgtct gattcagtgg gtaaatttgg gagtccaact agtgattctg aaagagcaat
    30721 gataacagaa gatgcaaatg aaatattaat ttgtatattt tcttttagtc aagaatctga
    30781 tattgaagaa tattttggat atgctaagga gttacttaaa gaatatgctg atggaaaaga
    30841 tatagaaact aaaataatta aatagaatct atataacaac gttatgttaa gctaaaattc
    30901 aatataaaaa taagttaaga taaagaaact aacataaaat tagtttcttt tttaaaagag
    30961 tagtttagtg ttacaaaaac agatagataa cccacgatta ttaaaagtat ggtaatatat
    31021 taatatagca gagattgtat ttaagggatt ttgaaagggg tgatttataa tagtcatttt
    31081 gtctaaaaga taaaatggtt ttatggaaat aactatatat ttaaaaatat aaacaatgat
    31141 acagtaattt gggtatataa aataaggcat acatggtttt gtttaaaaac caataaagta
    31201 cataggtaat tacaatgtaa ggaaggattt aattatgatt gatggaaaag aggtaaaaca
    31261 agaaagtgga caaaaaaatc taaaatttaa gatagctata gttgtaggtg ttttgtttgt
    31321 agcttatata ggaatggcaa tcttttttag aaatcatttt tactttggaa cgactattaa
    31381 tggcgtaaag gcttcaggaa aaacagttga gcaagtaaat gatttgttaa catctagtac
    31441 taattcctat actttaaact tagaggaaag aaacaataag aaagaacaaa taaaagcaaa
    31501 agatattgat ttaaaattta caccagatga taaagttcaa aagataaaag atagtcaaag
    31561 cgctattgga tggattggtg gaatatttaa atctaaagag tataataata tgataactct
    31621 ttcatataat aaagatttac tagaaaaaag gtttaatgct ttatcttgtt ttgatagtaa
    31681 aaatgttgta gaacctaaga gtgcttatcc taagtatgat agcactaaaa acgcttatgt
    31741 tatagtacca gaggtacttg gaaacaaagt gaaaaaagat gatttttata agttagtaca
    31801 aaactcaatt aattctggga atacttctat ttctctggat gaaagtaaag tttatgaagc
    31861 accaaacaac acaaaggatt cagaaaagtt gaaacaagct attaagacat tagataagta
    31921 tgttggtgta gtcgttacat atgattttag tgaccgtaag gaaactttag atggttctga
    31981 aattaataaa tggttaaaag tagactcaga aaagaattat gacataactt ttgataatga
    32041 tgctatggta gaatatacaa gaagtttaag taaaaaatac agtacttttg gtgactcaag
    32101 accatttaaa actgccacag gtaaagatat tacaatttca ggtggaattt atggttggtt
    32161 gatagataaa aagaaagaag ctgaagcatt agttgatgtt gtaaaagctg gtaaaaatgt
    32221 tacaagagaa cctatttaca tacaaaaagc tgtatctcgt aataaagatg atattggaaa
    32281 tacatatgta gagattagtc tttctggcca acatatgtgg ttctttaaaa atggagaaat
    32341 gcttgtatca acagatgttg taacaggcga tttagctaga ggatttgcta caccatcagg
    32401 tatatatcct cttaattata aggctagaaa tgtttcatta actggtcaag gttacagttc
    32461 acctgtaagt tattggcttc catttaatgg gaacataggc attcatgatg ctacttggag
    32521 aaattctttt ggtggaggta tatataagtc ttcaggttca catggatgtg taaatacacc
    32581 tttttctaag gcaaagaaaa tatatgaaaa tatagaacca ggtacaccaa ttattttata
    32641 ctagtaaatg tgttgctaac agttaagcga agtcatctta gtttatttag gatggcttcc
    32701 tttttatata aagcttattg aagtttacag aaagactact taaaaggaga aatacataat
    32761 gaatgaaaaa ttaactaaaa ttttagaaga agctaagaat gatgatgaat ttagaaagga
    32821 acttataaaa acaagagaac aacgtgatgt tatggattca ttttgtaagg ttgttacatc
    32881 tagaggttat gatataacag taggggaact tttttccatg ggagaagaat atacatcaaa
    32941 cctactgaaa agtgtaaatg gaggagcagc atatcctatt gaagattgga gtgattggta
    33001 cgaggatttt tttataatgt tgtcgaatat atagagtaac taatgatatt atctacttaa
    33061 gattaataca ttgccttaaa ataaaactat tagaactaag tgtaagtttt atcaatcttt
    33121 aaaaatattg atttatttct aactatatat aaaaaattta gattgaaaat aaggtgacaa
    33181 aaatcgatat agcttgatga tttataaaag ttagataaaa tagtgcgaga tatttctaaa
    33241 aattcaaaaa aaacaataaa cttcaagggt tagaggttga aaaagaatat aaaaaacttt
    33301 tatatgtaat aaattaaatt aaaattataa aaaaaataaa ataaaaattt aaaatgtcga
    33361 catagcgtta attttaaaac aaaataacgc tattgacaaa ctatatcgct ttaaattata
    33421 attattgaaa ataatggtat ttaaaaacca tactcatgtc atagtctaaa agtaagtcaa
    33481 aaaaggatgt aaatctaggc aagtactatt ctaaaatatg ttttattaag aaataaacgt
    33541 tgaaaaatat taattattat ataaaaatgc tgtttatact ttaacaatga ggtagtaatc
    33601 taattagaat aaaaaaatct aattggttat atacatagta aaaaggtaag agaggtgatt
    33661 tggatggctt ttgaaataat aaaaagcatt gttgaggcag agcagacagc agacagtatc
    33721 aaagtaaaag ctgttactga tgcagagtct atcagagctg atgctgtaaa caaatgtgaa
    33781 agcatatttg ctgatgtaag aaaacaagca aagcttatgg aagaaactct tattgagaag
    33841 gcagtcaccg acagtagagc agaggttgat aaaatcttag ctaatgctaa aagtgaatgt
    33901 ctgaaaattg aaaaaactgc tgaagaaaga aaaagtaagg ctattgaagc ggttattgga
    33961 aaggtagtga gataatatgg caatagttaa tatgaacaaa atatctattg ttggcttaga
    34021 agctcaaaaa agccagattt taaagctact tatgaaccgt gggtttgttc agattgatga
    34081 tagtgccttt cttactgagg aggaggaatt aaaaaattat cttgtaaaag atagtgaaga
    34141 atctgaagtt ataatgctag agcagaaaat gtctcatgta aaccaagcaa ttaatattgt
    34201 ttcttcacta gtgaaacaaa aaaaatccct acttgcacca aaaagagagt ttaacaaaat
    34261 tgatagtgaa aaagaagggc aagtttatgc tgaagctctg gagttaaaca atttgcacaa
    34321 ggagataggt acaataaggt ctaacataaa cttacttaac aacaataaaa gtatgttaga
    34381 accatgggtg aactttgata ttcctcttga aaatatggaa actaagtata caaaaactat
    34441 acttggaact gtgcccataa gcgcaaatat tgatgcttta agagtaaaat tagaagatga
    34501 aataccagaa agtgttattg ggattgtcaa ttctgacaag ttaatgaact atgtttattt
    34561 aattacttta aaagaaactt ttgatgatgt tttagaagtt ttaaaagaat ttaatttttc
    34621 agtggtttca ttaccaaatg aagaaggtac accgttacaa gctatcaaga aatacaatga
    34681 aatgataatt aggcaggaaa acgtttctaa ggacaaaaca gaggaaatta aaagacatgc
    34741 agatagtata cacatgcttg aaaacctata tgatttttac actatagaaa gagaccaaaa
    34801 aaagattata gagagacttg tcaaaacaaa aacaacattc tgtcttaatg gttggttacc
    34861 aagcaaaagg gcagatgaat taattaaaga aattacagaa aagtttgatt gttgtgttca
    34921 gactgaagaa ggaagtaaag aagaaggctt cccaattctg cttgaaaaca ataaattagt
    34981 aacacctttt gaaagtgtaa ctaacatgta cagctatcca agtacaaaag atattgaccc
    35041 aaatacaatt ttaactttct tctttgttgt attctttgga atgatgctaa gtgacgccgc
    35101 atatgggtta ataattgcag tagtctgtgg atttgttgta tataaattaa agatacaaaa
    35161 aggtgaagga aatttaatta aacttattgg tatctgtgga gtttcaacta ctgtatgggg
    35221 tcttatattc gggggtattt tgggggattt aattcctata aaagcattaa ttaatccttt
    35281 agaggatgtt atgacactta tgggaatgtc gttactgttc ggtattatcc atatatatgt
    35341 aggtttggga ataaaagcat atacattaat aagaagtgga aaggtaatag atgcaatctt
    35401 tgacataggt ttttggtatt tgtgtataac aggtgtatgt ctacttataa taccttttgt
    35461 tgcaggggac ataggtgtat tttcagaagt aggaaaatat ttagcaatta taggtgctat
    35521 aggtcttgta cttactcaag gaagaagctt taaaggtatt cctgttaagc tttttaaagg
    35581 attttcaagt ctctatggca taacaagtta ttttgctgat atattatctt atacaagaat
    35641 tatggcgctt tgtttaacaa caggagttat agcacaagtt atcaaccttt taggggctat
    35701 agctggtccg atacttgcag ttgtaatcgg tgttgtagga cacacaataa accttcttat
    35761 aaatgcacta ggtgcatatg ttcatacaag taggttacag tatgtagaat ttttcaataa
    35821 gttttatgaa ggcggaggag ttccttttgt tccgtttaag tataaaacaa aatatacaag
    35881 cattaataaa aaggagatgt aaatatggaa tttttaaacg cgttaggaaa tggtcaattt
    35941 ttggcaatat caggagctgc tatagcagca ttatttgcag gtatgggttc tgctaaaggt
    36001 acttcaatag caggtcaagc cgcagcaggt gttgtaacag aagaccctag taaattcggt
    36061 caacttctag ttttacaact tcttcctggt acacaaggtc tgtatggatt tatcatagct
    36121 ttccttattt tatcaaaagc agggattatt ggtggagatg caataccaac atcagctcaa
    36181 ggtttacaac ttcttatggc aggacttcca attggtttag ttggtttagt atcaggtata
    36241 gcacaaggta aagcagcagc agcaggagtt ggaatagttg caaaaagacc tgaagaactt
    36301 ggtaaagcta taacatttgc agcaatagtt gaaacatatg ctcttttagg actacttgtt
    36361 tctttcttag cttacaatgg aattgcaatt ggttaattat aaattataat tcataagtaa
    36421 aaaaaatgaa aaggaggaag cactcctatg ggtaatgaac aaaaaatgat tgacagaatt
    36481 atagctgatg ccaaacaaga agcacaagaa atattggata aagctaaaag tgaagcagat
    36541 ttaaaagtaa attcagctaa tgaaaaagct gaaaaggaaa tggcttccta tacaaagctt
    36601 gcagaggcag aagcagaaaa agcggcttct aaggaaatat caggagctta tatggaggct
    36661 aaaaagcaaa tattatcaaa aaaacaagag attttagaag aggtaatttt agaagctaaa
    36721 aacaaacttt taaatcttaa agataatgaa tatgaagaga ttatactaaa catgattgaa
    36781 aaatcaaatt gtacagatga ttcagagata gtattatcta aaaaagataa aaaaacatta
    36841 aaagatgtgc tatcaaaaaa aggcattaaa gtgtctgatg aaactagaga tattactgga
    36901 ggtttcatag ttaaaaaagg tgacattgag tacaattatt catttgaagc cataattgca
    36961 gtagagcatg aatatattga acaaattgca gcagaaattt tgtttaatta aggaggggtt
    37021 aaaaaatggc agaagaaaaa acttatccct atgccgttgc acgaataaga gtgcttgaaa
    37081 aacagcttct taacagacaa atgtttattc aaatggcaga agccaaaagc cctgaagact
    37141 ctttaagaat tatagctgag gcaggttatg ctgatacatc aaataataat atacataatt
    37201 ttgagaatat tttgactcaa gaattatcaa agacatatga aattcttaaa agtctagctc
    37261 ctgaagaaaa atttgttgat gtatttcttt ataaaaatga ctatcacaat ttaaaagtgc
    37321 tgattaaaga agaaatttct ggtgtagatg gtgagaaata tttaattgat ggaggtacaa
    37381 ttcctcttat taagcttaga gaatctttag caaatcgtag ttttagtgat ttacctaaga
    37441 taatgtcatc agctatatta gaggcatttg atgtttataa taagacacaa aatggccaga
    37501 tggttgatat tgttctagat aaagcaacct ttaccagtat gaaagaaact gctaaagaaa
    37561 gccaaaacaa atttgtaata gattatgtta tgaaagtttg cgatttaaca aatctaaaga
    37621 gttttataag agtcaaaaat atgaagaaag attttgatat gtttatgaat gtgtttgttt
    37681 caggtggctc acttgataag gaaaaattct tagaagcttt ttcctcagat actccagcat
    37741 catgttttaa atctacttct tactctgatg tctgtaaaaa tggcatggat agtggtttta
    37801 ctgtctttga aaaactttgt gatgactatc ttatggaata tgttagagga gcaaaattta
    37861 agcctcttac attagaacca ctaattgcat atttatatgc taaagaatca gagataaaaa
    37921 caattagaat tattttgact agtaagctta ataatataga cgcagatact ataaaagaaa
    37981 ggctaaggga tgcttatgta taaggtagga gtagttggag ataaagatag catcatggga
    38041 tttttagctc ttggaataga tatcttccca gcctatgata gtgacgaaat taaaaaaagt
    38101 atacataagt tggttgaaga tgaatatgcg ataatttata ttacagaaca ggcatcattg
    38161 cttgcaaaag aatcaatagc aaaatacaaa gattaccaac ttcctgccat tatagttatt
    38221 ccaggtattg gaggaagtat gggattaggt atgaatgaag taagagaatc tagtaaacgt
    38281 gctataggtg cagatatatt atttaaagaa tagatgaaag ttgaataaaa gacaatctca
    38341 aattttattg gtgctaaaga tatgtgctaa taaaatatag gagggaatat ttaaattttg
    38401 ataaagtata tgattgcagg tgagagaata tgaaaacagg cacaattgta aaggtgtcag
    38461 gaccgcttgt tgtagcggag ggcatgagag atgcgaatat gtttgacgtc gtaagagttt
    38521 cagataaaca tcttataggc gagattatag aaatgcatgg agataaagct tctatacagg
    38581 tttatgagga aacttccggt ctaggaccag gggaagaagt agtttctctt ggaatgccta
    38641 tgagtgttga acttggacca ggtcttattt caactattta tgatggtatt caacgtccac
    38701 ttgaaaaaat gtatgatatt tcaggtacta atataactaa aggtgttgaa gtatcttcac
    38761 ttgatagaac aactaagtgg gaatttaaac ctaaaaaatc tgttggtgat aaggttgtag
    38821 caggagatat aattggtggc gttcaagaaa cagcagtagt agaatgtaaa attatggttc
    38881 cttatggagt taagggtact ataaaagaaa tatattctgg tgaatttact gttgaagata
    38941 cagtttgtgt tattacagat gaaaaaggta atgatatacc tgtaactatg atgcaaaaat
    39001 ggccagttag aaaagaaaga ccatatagag aaaagaaaac accagattca cttcttgtta
    39061 caggtcaaag agttatagat acatttttcc caatcacaaa aggtggggta gcagctatac
    39121 cgggaccttt tggaagtgga aaaactgtta cacagcatca gcttgctaaa tgggcagatg
    39181 cagatatagt tgtatatata ggctgtggcg agcgtggaaa tgaaatgaca gatgttcttc
    39241 ttgaatttcc tgaattaaaa gacccaagaa caggcgagtc acttatgcaa agaactgtgc
    39301 ttatagcaaa tacatcagat atgccagttg ctgcacgtga agcttctata tacactggta
    39361 ttacaatagc tgaatatttt agagatatgg gatatagtgt tgcacttatg gcagactcta
    39421 catcaagatg ggctgaggct cttagagaga tgagtggtcg tttagaggag atgcctggtg
    39481 aagaaggtta tcctgcatac ttaggttcac gtcttgctca attctatgag agagcaggaa
    39541 aggtaaattg tctaggtatg gatgaaagag aaggaacact tacagcaatt ggtgcagttt
    39601 ctcctcctgg tggtgataca tcagaacctg tcagccaagc aacgcttcgt atagtaaaag
    39661 tattctgggg tcttgatgca tctttagcat ataaacgtca tttcccagca attaactggt
    39721 tgactagtta ttcactctat caggaaaaag ttgatgtatg ggcagataaa aatgtaaatg
    39781 ataatttctc agctcaaaga gctcaagcta tgaaactttt acaagttgaa tctgaacttc
    39841 aagaaatagt tcaattggtt ggggttgatt cactttcaga tggagataga ttgatactag
    39901 aggttactcg ttctataaga gaagacttcc ttcatcaaaa ctctttccat gaagttgata
    39961 cttatacatc acttcataaa caatatagaa tgatgggact tatattaaaa ttctataagt
    40021 cagcacaaga tgctatcaag caaaatgtta ctataaacga tattatcaag ctttcagttc
    40081 ttgaaaagat tggtcgtgct aagtatgtag aagaaggcga tatagatgca gcttatgatt
    40141 caattgaaga tgaaatagac aacgagcttg cagaccttat taaaaagagg ggggctgaag
    40201 aactatgata aaggaatata agtcaataca agaggtagcc ggtcctctta tgttaataaa
    40261 cggcgtagaa ggtgtaaaat ttgatgaact tggagaaatt gaactttcaa atggagaaat
    40321 tcgccgttgc aaagttctag aggtaaatgg agatactgca cttgttcagc ttttcgaaag
    40381 ctcaacaggt ataaaccttg ctgaaagtaa agttcgtttt ttaggaaaga gtcttgactt
    40441 tggagtttca ccagatatat taggccgtgt ttttagtgga atgggtagac ctattgatgg
    40501 aggtccagaa attataccag ataaaagact tgatataaat ggagctccaa taaatccagc
    40561 agcacgtgat tatccggccg aatttataca aactggagtc tctgctatag atggacttaa
    40621 tacattggtt agagggcaaa aacttcctat attctcaggg tctggtttgc cacatgccaa
    40681 tcttgcagtt cagattgcaa gacaggcaaa agtacgtgga actgattcta agttcgccgt
    40741 tgtatttgct gcagttggta taacttttga agatgctgaa ttttttataa gcgattttaa
    40801 agcaactggt gcaatcgacc gttctgtagt gtttgtaaac cttgcaaatg acccagctgt
    40861 agagcgtgta tctacaccta gaatggcact tacagcagca gaataccttg cttttgagca
    40921 agatatgcat gtacttgtta taataacgga tattacaaac tatgcagagg cattgcgtga
    40981 agtttcagca gctaaaaaag aagttccagg acgtcgtgga tatccaggat atctttatac
    41041 tgaccttgct actatgtatg aaagagcagg aaaaatgaaa gggcatgaag ggtctataac
    41101 tatgattcca atacttacaa tgcctgagga tgataagaca catccaattc ctgaccttac
    41161 aggatacatt acagaagggc aaattatact tagccgtgaa ctttataaaa aagatataca
    41221 gcctcctata gatgttttac catcactttc tcgtctaaaa gataaaggta ttggtaaagg
    41281 taagactcgt gaagaccatg cagatacaat gaaccagctt tttgcagctt attcaagagg
    41341 taaggatgca aaagaactta tggctatact tggtgaatct gcactttctg agattgattt
    41401 aatgtatgct aagtttgcag atgaatttga gaaagaatat gtttcacaag gatttagaac
    41461 tgatagaact attgagcaga cattagaaat cggatggaag cttcttagta tgttaccaaa
    41521 aagtgaactt aaacgtatcc gtgatgaata ttttgaaaaa tatatgccta aggagtgata
    41581 tctgtggcta gattaaatat aaatccaaca cgtatggaaa tgactagact taagaaactc
    41641 cttaaaactg ctacaagagg tcataagctt cttaaagata aacttgatga acttatgaaa
    41701 caatttcttg agattgtaag agaaaataag cgattgcgtg aagaagcaga aaatgctctt
    41761 gatactgctt ataaaaactt tataatcgca agagcagtta tgagtcagga gtatctgggt
    41821 tcagcactta tgatgccaaa gcaatcagtt tcagtagatg tttctacgag aaatataatg
    41881 agtgttgatg ttcctgtgtt tgactttaaa actgaaaaca atcaaagtga tatttatcct
    41941 tatggtcttg cttttacatc aggtgaatta gattctgcta tggaagcttt ctcagatgct
    42001 atgcaaccac ttttaagact tgcagaaagt gaaaaaagtg cacagcttct tgcacaagag
    42061 atagaaaaga cacgtagacg tgttaatgct cttgaaaatg taatgatacc aaattacatt
    42121 gagactatta agtatatagc tatgaagctt gaagaaaatg agagagcaag tactactaga
    42181 cttatgaagg ttaaggatat ggtgcttaaa aaagcacttg aagaaaagaa aaaaaatgat
    42241 ttggttgtat aattaaatac ttataaaaat aagtctaaca ttctacttta tagaggttag
    42301 acttattttt tatgtttaaa ttaaaatgta gatactataa aaattaatat attgtttaaa
    42361 aacagactgt atttaactaa aaataatata tttaacaaaa atttctaaaa attaacgaac
    42421 tgttaattta cacttaactt tatattaaca aattaggtgt attatataaa cataagcaaa
    42481 aacaataaaa aacaaataaa aaaaggaaaa aacacaaagg gggaagatat gaaagaattt
    42541 atatttactt gtctgtgcat tgtagtgatt acatttatga caagttgcat atcaacaaca
    42601 ccacaaacta ctcaaggaaa aggaaataat aaacttacaa tatatgcagc actagaggaa
    42661 gagcagatag cttattatat ggaaggtttt agagaagagt atcccaatat agatgtagag
    42721 atagttatgg attctcatgg tgtaatagct tcaaaactat tagcagaaaa agataatcca
    42781 caagcagata ttatatgggg attatcagca atgaacatga tagagctaaa aaaagatggt
    42841 gcacttgaag catataagcc taaaaattat gataaagttg ataaaaaatt taaagatact
    42901 gaagatgatg tatcttgggt aggaatgagt gtcccagaga cagcaatagt agttaattat
    42961 aaggaactta agaaactagg tatagatatt ccaaaaagtt atgaagattt aataaaacca
    43021 gaatataaag gacttataac aatgccaaat ccagcatcat ctggaacggg gtatcttaca
    43081 gtatcagggc ttattcaaat gatgggtgaa aaagatgctt acaattacat ggataaatta
    43141 cataaaaata tagctacata tacacactca ggttcaaaac ctgcgaagtt agctggctct
    43201 ggagagtatc caataggtat tacacttgga tatagagcta cacaacaatt atctagtgga
    43261 gaacctatag aggttgtatt tccaaaggaa ggttcaggtt gggacttaga agcaaatgcc
    43321 ttagttaaaa aagataatat aaaaaaagaa gcaaagctat ttttagattg ggcaataagt
    43381 gattcagcta tggatagata tgcacatgaa gtagcagtta cttccataaa gacatataat
    43441 ccagttccaa aaggttattc taaacaacca tttgaacagc ttataaaaga agttgattta
    43501 aactctgctg ccaaaaacag aagtacagta cttaaaactt gggaatcaaa atacggttca
    43561 aaatctgaaa gtaagtaagg tttaaattaa taatttagat gaaagaattt tagaagaaaa
    43621 agtaggatat aaaatttaaa ataaaaaaat aactatataa ggagagataa gatatgagtt
    43681 acttaaaaat aaataatgtg tttaaaagtt atgaccaaaa aagggtttta aataatatta
    43741 gtttggatat tgaagaaggg gaatttctat gtttactcgg acctagtgga tgtggtaaaa
    43801 ctacattact tagaataata gcaggtcttg aagatgtaaa tagtggaact ataattctcc
    43861 aggataaaga tattacaaat ttagagccat caaagagagg ttttggtata gtatttcaat
    43921 catatgcatt atttccgaat atgactgctt ataacaatat agcttttcca ctaaaagaga
    43981 gaaaagtttc aaaagaaaaa atagataata aagttaagga agtacttgaa acagttggat
    44041 tgactaatga agctcataaa tatcctaaag cattatcagg tgggcaacaa cagaggattg
    44101 caatagcaag agcattagct cttgaaccaa agtttttact tttagatgaa ccaatgtccg
    44161 cattagatgc aaaggttaga cacaagctta gaatggacat taaaagatta caaaaagaac
    44221 ttaatataac gacaataatg gtaacacatg accaagaaga agctataact atggcagata
    44281 aaatagctat tctaaatgga ggagatataa tgcaaatagg gactccagaa gaaatatacc
    44341 aaaatcctca aaacctattt acagctcaat ttataggaga tacaaactgt tttgataatg
    44401 gagattctat attaactgta agaccagaat atgttcaaat agagaaatcg acaaaagaaa
    44461 actaccaagg tataatatca aatatagagt ttagagggaa tttattgaga gttgaaataa
    44521 aagataaatt aaatgaaaat ttcataataa gtgatgtttc tataaaagag tgggttaatt
    44581 taaatttagt agaaggtgac cttgtaaaaa taagtataga cgaaaaatac tatttaaagt
    44641 atccaaaagt taaaggagca taagtgtatg aataataatg ctataagttc taaaagtata
    44701 agtcttaaat caaaaatgaa tttagatata aaaaaatgta tgcttacagt attagttatg
    44761 tctttagtaa tatttttatt agcacctata atatatctat ttgtacaggc ttttttagat
    44821 aaagatggag tctttgtagg attacaaaac tttcaagaat acttggcttc tgaatctcta
    44881 ttaaattctc taaaaaatac agtatttgta tcagttatat caactgtaat atcactaaca
    44941 cttgcattag tatatgcata tgcattaact agaacaaaga ttaaatttaa aggttttttt
    45001 aaatcattag ctatgtttcc tatgtttgct ccaacaatga tgcatgcaat aggtctagta
    45061 tatttatttg gaaatcaagg tttggtaact aatttacttg gagtgcatgt tccaatatat
    45121 ggagcagtgg gaataatatt ggcacaaatt atatatgtat ttcctcaatc ctttcaaata
    45181 ctttatatat ctctaagttc aaccgattat agactatatg aagctagtaa tactatgggg
    45241 ataagcaaga taagagaatt tataagcata acaatcccaa gtataaaata ctctttaata
    45301 agttctgcat tcgtatcatt tacattatgt tttacagatt ttggagctcc aaaggtgata
    45361 ggaggagagt ttaatgtatt atcaatagat gtatataagc aaattatagg gcaacagaat
    45421 atgccaatgg gagcaactgt atcaatatta cttttaatac ctgcaataat atcgtttata
    45481 gtaactaaaa aaattgaaaa aaatcaaaac tcatatataa cttcaaagtc aattccatat
    45541 aaggttaaaa ctaataagtt aagagatagt attttaggag gggttgtagg aattattgca
    45601 tttggtataa tagctgtaat gttaactgta atcttatcct cattaatatc agtttggcca
    45661 tatgatttaa gacttacact ggaacacttt tctatgaaaa acagtattga tggaattaaa
    45721 ccatttataa attcaataaa aatgtcacta ttgacagcta ttctagggac gagctttgtt
    45781 ttcttatttg catatttaaa tgaaaaaaca gaaaaagctc catatttaaa atcatttggg
    45841 tattttttaa gtacattgcc tatggcattg ccaggattag ttttaggtat agcttatgta
    45901 ttatttttta ataaattaga gtttaatttt atggatacaa tatacattaa aaatttgttt
    45961 aatggattat atggaacatt acttataatg gttatatgta atatagttca tttcttttca
    46021 gtagcattta taacagctac tactgctatt aagaaagtag ataaagaatt ggacatggta
    46081 gctaaatcaa tgggaataaa cagcgcatca attcttaaga acgtaactat accactttgt
    46141 ttgccagcag ttttagagaa ctttatgtac ttttttctaa actctatggt gacaatatca
    46201 gcactagttt ttatatatac tgcaagttca aaagtagctg caatatcaat tgtacaacta
    46261 gatgataagg ggagtactgc acaggcagca gcactagctg tgttaatatt agtcacaaat
    46321 atattagtga aagtaatata tgaatttata aagaatcgtt tagaaaaaag aacaaacttt
    46381 aattaaataa tttatcttat aaaaacacaa atttaaaaag tctattctta tgatttatta
    46441 tttaaagaat agacttttta aatttattat tgatattatt tattaatttt ctttttgtta
    46501 tcatacattt gagaatctgc acgacgatat gtatcaaata aagaaacatc tactttttca
    46561 tcaaaaatag catatccaat agacattgat aattttaatt cattagttga gttttcttca
    46621 act
//
LOCUS       DAELXX010000017        45706 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.017, whole genome shotgun sequence.
ACCESSION   DAELXX010000017 DAELXX010000000
VERSION     DAELXX010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 45706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45706
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.017"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..212)
                     /locus_tag="KPH29_000928"
     CDS             complement(<1..212)
                     /locus_tag="KPH29_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466555.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCN"
     gene            complement(713..1693)
                     /locus_tag="KPH29_000929"
     CDS             complement(713..1693)
                     /locus_tag="KPH29_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466556.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRVYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(1857..2729)
                     /locus_tag="KPH29_000930"
     CDS             complement(1857..2729)
                     /locus_tag="KPH29_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG1466557.1"
                     /translation="MIIKVSMTVLVLILLYILYKYETQHIETTEYTIFNKKIPKEFDN
                     FKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNLYG
                     LIIPLECYPYLFANNKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     VLAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITLKYEK"
     gene            complement(2893..3444)
                     /locus_tag="KPH29_000931"
     CDS             complement(2893..3444)
                     /locus_tag="KPH29_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1466558.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSYVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            complement(3464..4366)
                     /locus_tag="KPH29_000932"
     CDS             complement(3464..4366)
                     /locus_tag="KPH29_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBG1466559.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRTQINNKCDLCGECIKVCLKDAICIMT
                     EKMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDE
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            complement(4381..6753)
                     /locus_tag="KPH29_000933"
     CDS             complement(4381..6753)
                     /locus_tag="KPH29_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HBG1466560.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAEGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            complement(6972..7661)
                     /locus_tag="KPH29_000934"
     CDS             complement(6972..7661)
                     /locus_tag="KPH29_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466561.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMAGCMSPVSDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITDSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYIK
                     FIYNLFKSLTNTNLTEKEIFSMVEEEFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
     gene            complement(7902..9281)
                     /locus_tag="KPH29_000935"
     CDS             complement(7902..9281)
                     /locus_tag="KPH29_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG1466562.1"
                     /translation="MLNFMNELLVVLSIFIQFSIMNGILIYKKDSKKNEKIVFYIVLF
                     LIYIIIQISRLFNGGSYFSNINYLFYILAFIAWGGYYNYFYKMDYIKYSIFFYLFSVY
                     YKCLDAVIYRILFFAITGNVISSKESLLVPHLESIKIQIYSNLIIILIYLVFSFFRKS
                     IKLISQDKRNYVYLLFALLANTMNMLVNYVLKKLERFGSLHSEGYYFDNFVNPKLVGA
                     SSIFLILLFKEIIKENRLKSQAELIKNKLDMQYAHYLSIQESHMRVKKLYHDINNHIY
                     CIDNLRNNSKEINEYVNNLKDEIKTFKYIYNTGNMILDIIINEKSEICLKKGIKFTCS
                     INFSKVNFVKPIDVSSIFSNILDNAIEACDKIVDENINKYIRIKGTITKSFFVLKCEN
                     SKLNQLTFNKNILLTNKMDKFVHGIGIQSIKSSLQKYNGELLFENSIDKFILNIYIPL
                     EQDTDSWGD"
     gene            complement(9275..9988)
                     /gene="rgbR"
                     /locus_tag="KPH29_000936"
     CDS             complement(9275..9988)
                     /gene="rgbR"
                     /locus_tag="KPH29_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HBG1466563.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDDEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELEELKKHVLTCIKDIEINKESHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLGKIEKELNPYKFIRCHKSFIVNLRYVENIKPNAAILESGEEVPISR
                     YRYKEVKEKFLKFLGDTIC"
     gene            complement(10239..10799)
                     /locus_tag="KPH29_000937"
     CDS             complement(10239..10799)
                     /locus_tag="KPH29_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HBG1466564.1"
                     /translation="MSAMEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGI
                     FYFHKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYK
                     DIPPKLYSDSDKKNKVKSILFMNMFIFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVL
                     QIILLILYAVQLYRRLMYYLRLKKFK"
     gene            complement(10895..11698)
                     /gene="zupT"
                     /locus_tag="KPH29_000938"
     CDS             complement(10895..11698)
                     /gene="zupT"
                     /locus_tag="KPH29_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBG1466565.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            complement(11849..13012)
                     /locus_tag="KPH29_000939"
     CDS             complement(11849..13012)
                     /locus_tag="KPH29_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG1466566.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IEIPYVSAGGTGVIATIKGANPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAVLEGTIRLFNPELRKQIPGILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            complement(13072..14259)
                     /locus_tag="KPH29_000940"
     CDS             complement(13072..14259)
                     /locus_tag="KPH29_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466567.1"
                     /translation="MEAITAFCLVLISLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTIVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            complement(14622..16322)
                     /locus_tag="KPH29_000941"
     CDS             complement(14622..16322)
                     /locus_tag="KPH29_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1466568.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            complement(16578..16853)
                     /locus_tag="KPH29_000942"
     CDS             complement(16578..16853)
                     /locus_tag="KPH29_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG1466569.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIHAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
     gene            complement(16972..17523)
                     /gene="mobB"
                     /locus_tag="KPH29_000943"
     CDS             complement(16972..17523)
                     /gene="mobB"
                     /locus_tag="KPH29_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBG1466570.1"
                     /translation="MKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGYRVGVIKH
                     TCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQLYEDMDLI
                     IIEGYKNYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLNDYKNISKY
                     IESCIKNEILSISNVEIKNILKE"
     gene            complement(17528..18709)
                     /locus_tag="KPH29_000944"
     CDS             complement(17528..18709)
                     /locus_tag="KPH29_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBG1466571.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGEVFNKVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVIGDMIILNVKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILELEKKVKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGILGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGIHHIKAIFKGEFNKLSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVEIVI
                     R"
     gene            complement(18920..19654)
                     /locus_tag="KPH29_000945"
     CDS             complement(18920..19654)
                     /locus_tag="KPH29_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466572.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSVVIEKSDHEKSAVSLENFR
                     RIDLGEYVGVEESRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
     gene            20016..20921
                     /locus_tag="KPH29_000946"
     CDS             20016..20921
                     /locus_tag="KPH29_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG1466573.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKGVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
     gene            complement(21081..21923)
                     /locus_tag="KPH29_000947"
     CDS             complement(21081..21923)
                     /locus_tag="KPH29_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG1466574.1"
                     /translation="MTTSSKKLDTISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSSSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGIV"
     gene            complement(21907..22665)
                     /locus_tag="KPH29_000948"
     CDS             complement(21907..22665)
                     /locus_tag="KPH29_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG1466575.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            complement(22682..23152)
                     /locus_tag="KPH29_000949"
     CDS             complement(22682..23152)
                     /locus_tag="KPH29_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG1466576.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDELLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            complement(23152..23895)
                     /locus_tag="KPH29_000950"
     CDS             complement(23152..23895)
                     /locus_tag="KPH29_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane biogenesis protein"
                     /protein_id="HBG1466577.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLELGDMAIIGSYF
                     KDNYKDFGNVSVEHVKTFMDEIKKIREEL"
     gene            complement(23899..24312)
                     /locus_tag="KPH29_000951"
     CDS             complement(23899..24312)
                     /locus_tag="KPH29_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBG1466578.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDIECEYIILSDLYGASVCTAMLRLTNLHNVKLFSGMNLNMVLELLTR
                     FSDELTVEDIEQLVEESRRGIQSVTLQIKEEEEVF"
     gene            complement(24338..25090)
                     /locus_tag="KPH29_000952"
     CDS             complement(24338..25090)
                     /locus_tag="KPH29_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG1466579.1"
                     /translation="MDEKRQKKEMDLEVNSFVPLYQQLYDNIKKQIASGIYKPGDKLP
                     SEGDLCKEFNISRITVRNALNELVKEDILCKKRGKGTYVTIPERIEATCAGNSFTNSC
                     HRINAKPSTKIISVLIKKADKQVAEALSIELEEKVICIKRLRLIDEVPVIFEVDYFRI
                     DYMFLLKEELEDKSLMEVISNNISTLPKRVENIFEVKHSNKEYSDYLKCASNMPLLKV
                     RQSVYTENNDVLYYNEQFIRSDKYKYAVSAEI"
     gene            complement(25330..26724)
                     /locus_tag="KPH29_000953"
     CDS             complement(25330..26724)
                     /locus_tag="KPH29_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBG1466580.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSRNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTALPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNIPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGFDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVVWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            complement(26744..26953)
                     /locus_tag="KPH29_000954"
     CDS             complement(26744..26953)
                     /locus_tag="KPH29_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HBG1466581.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            complement(26982..28427)
                     /locus_tag="KPH29_000955"
     CDS             complement(26982..28427)
                     /locus_tag="KPH29_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG1466582.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSSNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTNKYKLSKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISVISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            28537..29331
                     /locus_tag="KPH29_000956"
     CDS             28537..29331
                     /locus_tag="KPH29_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBG1466583.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQYEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            29382..30704
                     /locus_tag="KPH29_000957"
     CDS             29382..30704
                     /locus_tag="KPH29_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBG1466584.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(30805..32022)
                     /gene="cdeC"
                     /locus_tag="KPH29_000958"
     CDS             complement(30805..32022)
                     /gene="cdeC"
                     /locus_tag="KPH29_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HBG1466585.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDECNPCNPCNPCKPNPCNPCKPNPCDDKCECHDKCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSIPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNAECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(32205..32385)
                     /gene="ssrS"
                     /locus_tag="KPH29_000959"
     ncRNA           complement(32205..32385)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="KPH29_000959"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(32500..33405)
                     /locus_tag="KPH29_000960"
     CDS             complement(32500..33405)
                     /locus_tag="KPH29_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466586.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN
                     DELNYEDEELDCEDKELDDDELTDEGEMEDNEDELVEDKNTGYENIENDEDIVYDEEL
                     YNINEQESEYNYNEKNTKTRAKKKR"
     gene            33617..34015
                     /locus_tag="KPH29_000961"
     CDS             33617..34015
                     /locus_tag="KPH29_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466587.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGGQQKKSKGLFKKTKKSNPHE"
     gene            complement(34138..35154)
                     /gene="ccpA"
                     /locus_tag="KPH29_000962"
     CDS             complement(34138..35154)
                     /gene="ccpA"
                     /locus_tag="KPH29_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HBG1466588.1"
                     /translation="MKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEVIK
                     ETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANTYS
                     DKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYTVS
                     TSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKSLI
                     KYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVAGF
                     NDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRESVT
                     ERKK"
     gene            complement(35407..35607)
                     /locus_tag="KPH29_000963"
     CDS             complement(35407..35607)
                     /locus_tag="KPH29_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466589.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            complement(35641..35820)
                     /locus_tag="KPH29_000964"
     CDS             complement(35641..35820)
                     /locus_tag="KPH29_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466590.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            complement(36002..36208)
                     /locus_tag="KPH29_000965"
     CDS             complement(36002..36208)
                     /locus_tag="KPH29_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466591.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            complement(36256..36681)
                     /locus_tag="KPH29_000966"
     CDS             complement(36256..36681)
                     /locus_tag="KPH29_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466592.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            36853..37080
                     /gene="acpP"
                     /locus_tag="KPH29_000967"
     CDS             36853..37080
                     /gene="acpP"
                     /locus_tag="KPH29_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBG1466593.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            complement(37224..38228)
                     /locus_tag="KPH29_000968"
     CDS             complement(37224..38228)
                     /locus_tag="KPH29_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBG1466594.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFYEEK
                     "
     gene            complement(38242..39144)
                     /locus_tag="KPH29_000969"
     CDS             complement(38242..39144)
                     /locus_tag="KPH29_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBG1466595.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKKEK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEELINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            complement(39339..40514)
                     /locus_tag="KPH29_000970"
     CDS             complement(39339..40514)
                     /locus_tag="KPH29_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBG1466596.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            complement(40601..41446)
                     /locus_tag="KPH29_000971"
     CDS             complement(40601..41446)
                     /locus_tag="KPH29_000971"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HBG1466597.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            complement(41544..42341)
                     /locus_tag="KPH29_000972"
     CDS             complement(41544..42341)
                     /locus_tag="KPH29_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBG1466598.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            complement(42360..43370)
                     /locus_tag="KPH29_000973"
     CDS             complement(42360..43370)
                     /locus_tag="KPH29_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBG1466599.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            complement(43389..44171)
                     /locus_tag="KPH29_000974"
     CDS             complement(43389..44171)
                     /locus_tag="KPH29_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG1466600.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            complement(44230..45366)
                     /locus_tag="KPH29_000975"
     CDS             complement(44230..45366)
                     /locus_tag="KPH29_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HBG1466601.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLKPLASGDKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
BASE COUNT        14658 a         8209 c         4774 g        18065 t
ORIGIN      
        1 ttattacatt ttccacaaca atactcatat gtctccacat tatttggaat atttacaaat
       61 acaaattctt tacagttctc acaatacttt aatatagata tagttccaca agtgtaacat
      121 tcaaatatta cacaatcctc attttcctct actatttcaa actttttact atgttcacca
      181 cactctgggc acttgtgatt atttacattc ataaatactc ctccttatta gtatagttac
      241 atttcaaaaa cttaattaat gtattctata ttacttatta tatattctat aataaatcat
      301 attctaatat aataaaacaa attaaataaa aaaaaaccat aaattttaaa tttatggatt
      361 aattcttgta atgataactc tatttgagga tgtgagggag ggttatcatt acaatagggt
      421 attatattat ggtttttaag ctagtctttc tacacctcat atgatacata tgctatatga
      481 attttatatg atttttacat aaatatttta tgaaatttaa attttttagt cattttgaaa
      541 tttgtaaaaa gttctctaaa aatccacagc atcataaaca gccaaaattt aagtaaaaca
      601 tcataaaaaa atttgtccaa taattttaac aagtcatttc acctcattat aattattgac
      661 acaaacttaa ataaacaatc tataaatctt atataaatct taaactttac ccttattgat
      721 acacaatttc tagtttgtca attttatttt gcatctctaa gaaaatttct tcaacagaat
      781 tgttaaatat attgcctaaa tagactcttt tatctttttg aaatgaataa gattttacac
      841 taccatcaga agaaatatat aaatgactcc actctgctcc atattttgaa aacctctcct
      901 ttgtatcttt aaacctcata tcttctattt ttattgcttt ttctctgtct cctcttacta
      961 cagtgtgacc cctttttatt aaactggtta tttttatatc ttcttttaat ttaatacgtg
     1021 tatcatcatt ttttgaatat actttccata tcactttatg acgataatta tctaattctt
     1081 ttattaagct tctatcaata gtaaagctat ctttagttac cacaattaaa tcaaaatggt
     1141 ctaacatata ttgaaatatt tctctcatac ttttattttt taaaatatcc tcaccaataa
     1201 cctctataaa tctacagcct ttctcataca tgcagttcaa taaacttgtg atttcagaaa
     1261 aataatcatt tgtattatta tctccatcta tttctatagt aaagtacaaa ggagtaatgt
     1321 atgatttatt tcctgttatc tcaaatacac ttgatagtgt ctgtatatca tcttcaaacg
     1381 ttaagtatac attctcataa aacataaaaa attccataac tttttctttc atattatgga
     1441 tatttctata tctattggat aatatattta ttatatcgtc agtagtattt ttcccatcac
     1501 attcagccag tataacaatt tgcttccaac ttaagctatc cacatttccg atactttttt
     1561 tatatccatc ttcttcatca taaataggac caattataat gttttctttt ggccacagat
     1621 gaaccctagt tccatctttt aatttcggat atttattttt tacagactca taaaatctac
     1681 ttgtatcacc catgattctt tactcccccc catagtttaa atattctatg ctaaaaatag
     1741 cactttattt ttattaattc aacaattatt caaaattggt cattatatat gtttaaatgg
     1801 atttaacatc aaccttattt atatgttatt gatgtttata tatgttatat aaaatcttat
     1861 ttctcatact tcaatgtaat cttaactatc tctggtttac aattaagcct aattaaaact
     1921 tttgagcctc ctaagccttt atttaaaatc atagtgctat tatctttttc atattttccc
     1981 cagtcatact taggaaagaa ctctctattt ggagataata cacctccaac aaaaggtatt
     2041 cttattatac ctccatgaac atgtcctgct aaaaccaaat ctgctcccca ttttgcatat
     2101 ttttcaaaga aaaatggtgt atgtgctaaa agaatgttat attcattttc attaacttta
     2161 cctagattgt cttctacaaa attgtcactt agttctaatt ttttgttatt agcaaataaa
     2221 tatggatagc attccaatgg tataattaaa ccatataaat taagatggct attacctcta
     2281 tatattctta cagattcatt ttccatattt attattttct ttgaatatag ttccttaaaa
     2341 taatccttat atagatgttt atactttttt attaaagatt tttgttcatg atttccaatt
     2401 atatgatata cttcatattt ttcaaccaaa ttatctatta aatctaatgc aacttgaaaa
     2461 tttttatttt ctccatctac taaatctcct gttataaata taacatctgg gtttaatgat
     2521 tcaattttat ctatcaatac cttattattt ttatcaaaca ccttattatg taaatcactc
     2581 acttgaacta ttttaaagtt atcaaattcc tttggaattt ttttgttgaa aatagtatat
     2641 tctgtcgttt ctatatgctg agtttcatac ttatacagaa tatacaataa tattaatact
     2701 aatactgtca ttgaaacctt tataatcata ttctcttcct ctttattcta ttttgttaat
     2761 attattataa tgcttttatg ttttttttaa aacaataaca tcatcacata catataaatc
     2821 ttttcgacat tataaaaata agttatcctt ttctaaaatt ttcatttatt ttagaaaagg
     2881 ataactataa aattatctat aaacctctat attgtgaaat atagtttttc ctttatttaa
     2941 atttttatat atatgctcta aagttccatc aaacctaata gaatgtccag actctaaaat
     3001 ataagttttc ccatccacat taaggtcaag tttaccttct attacgagta catactcttc
     3061 taaaacataa ctatgtgtag atgaaacatg agtacagttt tcctcaagct caattataaa
     3121 aagttcaaac cttctttgag ggctaaaagg gtatattgga tataacttca tgttcccttt
     3181 ttgctcaaca ataggctcta cttcttcttt ctttataacc tcataggtat ttgtattttc
     3241 atttaatagt gtagtaaaat ttattttaaa tccagatgaa attttccata aagtagatac
     3301 tgtcggactt gattcacctc tctcaatctg tccaagcatt gctttactga cacctgtcat
     3361 ctttgaagct ttgtctaaac ttatgcccat ttcttgtctc aataacctta aattttctcc
     3421 cacattagga aatttattat ccatgttaaa atcctcctga tagctatttg tattctatga
     3481 tttttactcc tgtaagttct actatctctt ttaattttaa catatgattg ttacaaattt
     3541 catcaaattc atatatataa tcttcaagcc ctaatgcctt atatttatcg attccaaatt
     3601 tatgatatgg aagtaactcc atcttgcctc ctggaacatt ttgtttgaca aataatgcag
     3661 tatttcttat attttcttca ctatcattta ctccataaat aattggcact cttataacca
     3721 tagatttatt caactttgaa agcctacata tattatctaa tataatttta ttacttactc
     3781 ctgtatattc tttatgaata ttatcatcca tagattttat atctacaaat atgtggtcta
     3841 ttttctcaaa tacatcattt accttattcc aatcaaaata tccacaagtt tcaattgcaa
     3901 tatttatgcc tttatcataa aaaatatcaa ctaattctct taaaaaatca atctgaagtg
     3961 taggttcacc tccagaaaaa gttatccctc cattagattc aaagaaaaat ataaaatcct
     4021 tttcaacttc ctctactatt tcttcaacac tcattttttc ggtcattata caaatggcat
     4081 ctttaagaca taccttgata cactctccac ataaatcaca tttattattt atttgcgttc
     4141 tatcaaatag tgaacttata ttttgaggac aaacatctat acatctatta caggatacac
     4201 atttatcttt cataacacct aattttacta tgttactcca ggaatctgga ttagaacacc
     4261 acttacattt taatttacat ccttctaaaa atatagtagt ccttattcca tctccatcat
     4321 ttactgaaaa atgttgtact tttacaactc tacctttttt actcataatc tacctcataa
     4381 ttaaaaattg ctatgtgaat ttctagctat tatagcatct tgcatttctc ttgataaatt
     4441 aacaaattga gtactatatc cagcaactct cactaataaa tctttatatt cttctggatt
     4501 ttcttgagct tttaatagag tatcatttga tatcgtatta aattgtacat ggaatcctcc
     4561 taaagcaaaa tatgatttaa tcatagatgt tagatttcta agtcctcttt cagtttccac
     4621 caaatcttga ttcagtctta aatttagaag tgttccacca gtatgaacat catgatttat
     4681 ttttgatgca gatttcatgg ctgctagtgg acttgttgta tcagtaccaa caaacggaga
     4741 aactccctct gtcaaaggtt ttgtcgcttt tcttccacaa ggagttgcac ctacgatttt
     4801 tccagttggt acatagtttg atatacccat aaatgcacta ttaaatttag agccaaaaat
     4861 atccttgtat tttctagttt cagtataata aaaatcagaa acttcaattg ctaatgaatc
     4921 tacataatca ttatcatttc catacttagg aacatccaac gcaagttttc ttaattcttc
     4981 ataaccttcc caatcattat ttagtgcttt tgttaattct tctagcgtag tcaccttatc
     5041 ctcaaatact aattttttta tagctgccaa tgaatttgaa actacaccta accctatacc
     5101 agttaataca ggacctatat tatattttgc tcctccttta cttaaatcaa ctcccttatc
     5161 caaacatcca tctacacaag tagataaaaa tggtcttggt accatttctt tatgaatttg
     5221 ttgagcaaca gtagttccta taacagaact ttttacaaga tatgctaatt gctttaaaaa
     5281 ctcatcctta agttcttcaa aagaagtaaa ttctgtgata ttttttgtat ctaaccccat
     5341 tttttctcca gttaatctac tcttaccttc attcatagca tattctaaag ctgctgcaaa
     5401 attgatattt acggctgaag tccactgtcc tgtttttcta aaatgaggaa ctacacaacc
     5461 acagttactc caatctctag catcttctgc atcatatcca tcttgtaata acatttgtgc
     5521 ccctgcacta tcgctgtgta tagcagggaa accagttcct tgttttacca atttagcaac
     5581 tgccattacg aaattatctg gtgctccttg actcactcta acacttaatc ctggctggtg
     5641 agtttttaca ctttctgtag cctttaaaca catatatgag atatcattcg ttccatcagt
     5701 accatttctc tttttaccac caacagtaag attttggaat tgattatatc ctgcaaagta
     5761 atttgctgta tttgctgata tagtccatac ccattctgat aacttaatcc aaagtgcttc
     5821 tatcaattct tgagcttctg attcagttat tttaccttct ctagcatcat tttcataata
     5881 tggatacata tattggtcaa atctacctaa atttaatgcc aatggatttt ctgatagaat
     5941 accacctaat tgaacaaacc atacaaattg tattgcttca taaaaattag ttggagggtt
     6001 tgctggtaca ttcatacata tctctgaaat ttttataagt tctaactttc tcttttcatc
     6061 tgtttcttgc ttactcatct cttttgcaag atttgaatat ctttgtgcca atgtcataat
     6121 accctctgca actatagaaa cagatttgta aaaatctatc ttttcaatat tttctgaaac
     6181 tgatatatct aattctttaa tttttttatc tgcatcttcc ttaatccctt tatatccttt
     6241 tacaaacaat atatcttgat aatctggtgt tacttcacca actgcctgtc tccacttaga
     6301 atcattatct attattcctg tatctactaa tatcttagct gtatcttctg ggattcttgc
     6361 taagaaaact tcttccaaag attttccctt ccaatatgga aatatttctt ttcttataaa
     6421 ttctatttgt tcttttgata ttacatatgg gtcttgagtt cttttatcaa aagtatccat
     6481 ttctttgtct acccattgcc aagaaaactc tggtgtcaat atccctgttc ttctaaattt
     6541 tcctgctgta cctactatta attcattttc aaagattttt ataggaagtt ctttacatgt
     6601 ctccaaaaaa gctttagctc ttcttataca gatagcttct ccctcagttt ttttatgtga
     6661 ttctgtaaaa attctagccc tttcacaaca tatcataggt ttagaattaa tatattcttg
     6721 tctaaatgct tctattctat tagtttgact catttaaaat ccccctttgg ataatatata
     6781 ctacattttt gtatgttata acatacttta attatattat actgtccttt agcataaaat
     6841 ggaagcaaaa taactttttt tttcatctac cttgaacacc tatatattaa tacttttttt
     6901 gttaaattta taaaatctaa aaaaattttc tatgatatat tttctatatt gttatagtta
     6961 tttctaatat actaatgatt tgaaatatca aatttgtata ctagacttag ttttaaaacc
     7021 ttagttgatt gtttaacttt gttaacttca acatgtatat ccttcatata aggaagctcc
     7081 acaacaccta ctcccttatt aaattcttct tcaaccatag aaaaaatttc tttctcagtt
     7141 aagttcgtat tagtcaatga tttaaataaa ttgtatataa atttaatata tttatcactc
     7201 tcagataact tatcatcttc tggatactct ttagcacaga gaatatctat tgttactttt
     7261 ccttctttat cgtcttcaga catagaatag aataaacttc gaatattttt ttcaggtggt
     7321 ttttcagcac catttataga tactacaaag ctaccatctg attttgagtc agtaatacta
     7381 taaccactgt ctattatagc tttcgaaaat ctttgagata aatctttttt tacttcttct
     7441 ctacctttcg catcattaat cttttgtgag taattattaa atagctcgat agattcatct
     7501 tttttatctt caacttgttc tttcaatgaa tctttactaa tttgttctga gctacttaag
     7561 ttcttatcag atacaggaga catacatcct gccatcaatg ctaagctaag atttaaagcc
     7621 acaattattg ccactctatt tatattcctt tttactttca tacacaatcc tcccatcaca
     7681 ctaaccgctc taaagtatta ttcattgcat tagaatatat ttatattttc tttatattaa
     7741 agatttatta ttcaatacat atcaccttcc tatccaattc ttataactta ctattagtaa
     7801 aattataagc tccttaatac agaaataagg atgttatctt ttgttattaa aagataacat
     7861 ccttattttt ttgtatcaat acatatgtcc caactggtaa cttaatcgcc ccaactgtca
     7921 gtatcttgct ccaatggtat atatatattt agtataaatt tatcgatact attttcaaat
     7981 aataattctc cattatattt ttgtaaagaa gatttaatac tctgaatacc tattccatgt
     8041 acgaacttat ccattttgtt tgttaacaat atatttttat taaaagtaag ctgatttagt
     8101 ttactatttt cacatttaag tacaaaaaat gactttgtta tagttccttt aattctaata
     8161 tatttattaa tattttcatc cactatttta tcacatgctt ctattgcatt atccaaaata
     8221 ttagaaaata tgcttgacac atctataggt tttacaaaat ttacttttga aaaatttata
     8281 gagcaagtaa atttaattcc tttttttaaa catatttcac tcttttcgtt gattataatg
     8341 tctaatatca tatttcctgt attatagata tatttaaatg tttttatttc atcttttaaa
     8401 ttgttaacat attcattaat ttctttgcta ttgtttctaa ggttgtctat acaataaatg
     8461 tggttattta tatcgtgata tagtttctta actcgcatat gagattcttg aatacttaaa
     8521 taatgcgcat attgcatatc taacttattt tttattaatt cagcttgaga ttttaatcta
     8581 ttctctttta ttatttcttt aaacaataat ataagaaata tactactagc tcctaccaac
     8641 tttggattaa caaaattatc aaaatagtat ccttctgaat gtaatgaccc aaaccgttct
     8701 aattttttta atacataatt tacaagcata ttcattgtat tagctaataa ggcaaataat
     8761 aaatatacat aatttctctt atcttggcta attaatttta tagacttcct aaaaaagcta
     8821 aatacaagat atataagaat aattattaaa ttactatata tctgaatttt tatagattct
     8881 aagtgaggta ctaataagga ttctttagat gaaataacat tacctgtaat tgcaaaaaat
     8941 agtattctat atataacagc atccaaacat ttataatata cactaaatag ataaaagaaa
     9001 atggaatact taatataatc catcttataa aaataattat agtatcctcc ccaagcaata
     9061 aatgctaata tataaaataa atagtttata ttactaaagt aacttcctcc attaaataat
     9121 ctacttatct gaattataat ataaataaga aacaagacaa tatagaatac tattttttca
     9181 ttttttttac tatccttttt ataaattaaa attccgttca ttatagaaaa ctgtataaat
     9241 atagataaaa ctactaatag ctcattcata aaatttagca tattgtatca ccaagaaatt
     9301 ttaaaaattt ctccttaact tctttatatc tatatctact aataggtacc tcttctccac
     9361 tttccagtat tgcagcatta ggctttatat tttcaacata tcttaaattt actataaaac
     9421 ttttatggca tcttataaat ttatatggat ttaattcttt ttctatttta cctaaactat
     9481 attttatatc aaaattcttg tttattgtgt gtattagcat atcttttttt tgaacttcta
     9541 tatattttat ttcatttaaa taaatcttat atgtattaga cttatttttt attgtaatat
     9601 gactttcctt atttatttca atatctttga tacaagttag cacatgtttt tttagttctt
     9661 ccaactctat tggctttagt aaatacctat aagctcttac ctcataacct tcttgcacat
     9721 aatctattaa tgaagttaca aatattattt ccatttcatc gtctacttct ctaatctttc
     9781 ttgcagtatc cattccattt aatttttcca tttttatatc aagaagtaat atatctatat
     9841 cttttaaatt actttccagt aactcttctc cagatatata cttttgtatc tcatattcta
     9901 tacttatatc ttttaatata ttctcaaggt aatcttctat taaaagttgt gtttcttttt
     9961 catcttcaca aactgctatc ttaatcataa aatgcctcct tacataattt ctagttaata
    10021 caattaaaat atttataata tatttaaaga taattttgta tatctgatac tttaggataa
    10081 catttttaaa ttatttttat caatactcaa tccaatacaa ctattatttg atatttcaat
    10141 ttaaagacag aaattactat aattaaaaac agtactttac taacaaaaga agatgtctta
    10201 aaatattaaa aaaataattt taggacacct tcttttattt atttaaactt tttaagcctt
    10261 aaataataca ttaatctcct atataattgt actgcatata aaatcaacaa tataatctgt
    10321 agtacaatag cagttacaga tgctccataa ccactatata aatttcttat ttcaatattc
    10381 aataaatata taaatattac tactaacata aagaatataa acatattcat aaataaaatt
    10441 gactttactt tgtttttttt atcagaatca ctatataatt taggaggtat gtctttgtat
    10501 tctttcgacc atatatacca tgcagatgta cccataaaac taccaatact cctaaataca
    10561 agattccagc catttgattt atgttcatca aaataattat tctctacatc atagtagtaa
    10621 tcaacagtgt attttatttt tcttggactt cccttatgaa aataaaatat tcctccaaat
    10681 ttatttatct tatataaatt atatccttta gcttcaatat cttctaacca tttctctatc
    10741 ttatcaggtg aactatacca gtcaaatttg aattttattt tcttttccat agcactcatt
    10801 ctccaaaatg atatttttaa atagcttaaa aatctattta aataattata tcattttgat
    10861 actaatttta ggttacaatc atatatttaa aaagctattt aaatagtaat aaactaatag
    10921 ccataacacc cattcctgca atcaatccat aaatagataa atgatgttcc ccgtactctc
    10981 tagctgatgg caataattca tctagtgata tgaataccat aattcctcct acaattgcaa
    11041 atacaattcc aagagttata tcatttaaaa aatttctaag aagtacatat ccaataatcg
    11101 ctcctaaagg ctcagacata ccagataaaa aggaataata aaaagctttc tttttatctc
    11161 ctgtggcata ataaactggt acagatacag atataccttc tggtatattg tgtattgcaa
    11221 ttgcaattgc tatcgggata gctattgtaa catcatcaag agctgatata aatgtagcta
    11281 atccttcagg aaaattgtga attgctatag ccattgctgt aaatatacct gttctcaaaa
    11341 gagacttatt ttttttgata ttcttttctt ctatattctc cattttcttt atttcatgtg
    11401 gattttcttc ttgtggaatt aacttatcaa taacagcaat gatagccata cctataaaaa
    11461 aagctattac agttgaccaa cttcctaatt tctctcccat agattttgtc aaagcatctt
    11521 gtgctttagg aaaaatttct atcatagaaa cataaatcat aacaccagca gaaaatccta
    11581 agcttataga aagaaactta gtatttgttt ttttagcaaa aaatgctata caacttccta
    11641 taccagtaga taaaccagcc aataatgtta tcaaaaatgc aaatattaca ttattcaaat
    11701 aaatcacctt ctttttaatt gatattgatt atcattttct ttatgctatc atatatattt
    11761 tatatagtca atatttataa aaaatataca caataaaaaa gcagagatat aaacttagtc
    11821 tctgcttttt ctttatagta tattaaaatt atttatttaa gaaatccact gcatattgca
    11881 catataaagc agttccaatt tctagagcat cttcgtctat attaaagcat ccatgatgat
    11941 gaggatagca agcacctttt gactcatttc tagctcctac aaatgctagt gcaccaggtg
    12001 ctatattcat aaactcagct aaatcttctg ctcctgttac tttttcaaat agtgttatac
    12061 agtcttctcc aaatagctta attgctgcat ctgtagctat ctttgaacat tctttgtcat
    12121 ttataactgc tggtgttaag tacccatact ctaattcagc atctgctcta tatgcttctg
    12181 ctgtagattt tgctatacgt tctaaaattc caggaatttg ctttctaagc tctggattaa
    12241 atagccttat tgtaccttct aagacagctt cactcgcaat tacgttaaat ctagttccag
    12301 aatttaaaac tccaacactc acaactaatg gttccagagg acttacttct ctactaacca
    12361 tagattgtag attcataact attgctgaac ttgcaagcac tgcatcaaca ccttgatgag
    12421 gaagtgaacc atgaccacct ctacctttta cagtaatttt aaagaaatcg gcagaagcca
    12481 ttcttggtcc ttcttctacc gatatagtcc cagattctac atcagtccaa agatgtattc
    12541 caaacacact atctacacct tccatagcac catcttgaat catagctctt gcacctttac
    12601 caacttcttc tcctggctga aaaaataatt ttacagttcc attgatacta tccttaatat
    12661 catttaacac ttttgctgcc cctagaagca ttgatgtgtg accatcatga ccacaagcat
    12721 gcataagtcc ttcattttta gatttgtatt ctacatcagt gcattcaaca acttgtagag
    12781 catccatatc acccctaagt gctacagtct ttcctggatt agctcctttt atagttgcaa
    12841 ttacacctgt acctccagct gatacatatg gtatctctat tttatcaagt tcttctttaa
    12901 ctctctttga tgttctaact tcttccatac ttttctcagg attttcatga aattcccttc
    12961 taagcttaat tacatagtct ttataactct ttgttatttc tttaatatcc attccatacc
    13021 tccaagtagt acacattctt cttatttaaa atacttttta tcacttaaat actatattag
    13081 atttacaaag atacctgcta ttactacaga agctattgta actgtagtaa atcctccaac
    13141 aagcatcttt ggaagcatct tatccattaa aaattctttt tcttctggtg tttcagccaa
    13201 agcctttact gcctcttcag ttaatatata gtttggtgga aaaccataaa gtgatgtaag
    13261 cgaaactgaa aaagccattt ctttactttc acctaaaagc ttacctacta taatagagaa
    13321 tattcccata cctattacac caaatattat gattcctaca agaggaactg ccatttcaac
    13381 aagcattgct ggtgtagctt ttgctaatcc tgcaaatata aaagccatca atgatgtcat
    13441 taaccatcca aatgaaccag atttattaag tggttttcta tcaactattc caagttctga
    13501 tgctattaca ccaaatatta aacatataac atatcttgat acaacttcat ttgtaagatt
    13561 tgtaaattgt actgctaacc atgctacaaa tcctaatcta agtattatca tatatgtcgt
    13621 atcatatttt tctggtaagt ctggaattat cttaaatctg cttttttgtt caggtaaatc
    13681 cttaactttt tcttcatctg tatttacagt tacttttcca cttctaaagt ctgataaaag
    13741 tctttttcct tcttttttaa gcattaatgc tgtaatagga taacccgcaa atccttgtgc
    13801 aacatacatc aaaattgcaa gtactgataa acttgcaaga ccttttgctg tagcagcatc
    13861 tgccatcatt atagatgcaa ctagccctcc agtaagagga ggtgtagcta ttactgctgt
    13921 atcccatcca aatactattg tccctaatgc caaagcacct acacatattc ctactattcc
    13981 tgccaaggct attactattg ttttccattg acctgctaat tccttaatac tcatcatagt
    14041 tcccatatga gttattaaca aaagcatacc taattgagct aatggtgttc ctaaagcggc
    14101 taagtccaca atattttctg ggaaaaaagt ccagaatcct actataaata taattgctga
    14161 tacaaaaacc gacggaacaa aagctttagt ttttgctgaa actatatctc ctatcgtcag
    14221 tgatattaaa actagacaaa aagctgttat tgcctccata caaaatcccc cctcataata
    14281 aatgtatttt tataaaatgt atgtatagtt atccattatt ttactacttt tgaaaaacat
    14341 ttcaagtcaa aattcgattt ttttcataaa attcgataaa ttccataaat taaagttagt
    14401 tttctaaatt attctttttg taaaaacgtt atcctgacca ttggctattt aactctattc
    14461 ttacaaagag aattaaatgt aatttgcaaa aaataaaaca taggatacat tttattcata
    14521 aaacatctca taaatataaa atgaattgtc tttatgaatc ttaatatatc ctatgtttgt
    14581 aagtttaatg cgtatgttag ctatggtgta aaatttgtat actagttatt gtgtaaccta
    14641 ctatatactc catttttgtt tactaactct tcatgagtac ctctttcttc aattccttta
    14701 tccgtcaata ctatgatttc atcagcattt tttatagtag tcaatctatg tgcaacaact
    14761 aaatttgttc tatctttact taattcctct aaagactctt gaatttctct ttctgttaca
    14821 ttgtctaaag cagatgtagc ctcatccaat attattattg gaggattctt taaaaagatt
    14881 cttgaaattg aaattctttg tttttgccca cctgataatt ttacgcctct ttctcctata
    14941 taagtatcat atccatctgg caaagtctct acaaagtcat gtattctagc ttttttagca
    15001 gcagctatca tttcttcatc tgtagcatta gggttcccac aaagaatatt atctcttata
    15061 gttcctgtaa ataaaaatac atcttgttga actataccta tattttttct caatgatttt
    15121 aaactaacat cttttatact ctttccatca atttttatat cacccttatc aaattcataa
    15181 aatcttggca atagattaca taatgtagtc tttcctcctc cagaaggtcc aactaatgct
    15241 atagtcttac cagcttttat agataaactt aaattatcca aaacttgagt cttatcttca
    15301 tatgtgaaag atatattttc tatctcaatg tcacctttta cattttcaag ttcaatagga
    15361 tttttagctt ctttttgatg gtcttgttcc attatctcta tgaatctttt aaaacctgtc
    15421 ataccatttt gatattgctc tgtaaaattt attaattttt tcataggttg tataaacatt
    15481 tttacatata gtatataagc agcaaaatca cccaaagtaa tttttcctat ataagtaaaa
    15541 taaccaccag ctattacaac tacccactct aagatatcca tgtataaatt cataccagag
    15601 taatattctg ccattacctt ataagaatct tgtcttgctc ttctaaataa tttattgctt
    15661 cttacaaact tatttaactc ttctttctca gtacaaaaag acttagttac tttcatacct
    15721 gctatactat tttctaaagt agcatttaca tcacctatag taactctact cttcataaaa
    15781 gcaatattca tcttatcttt tttagctatt gcaaaccaaa ttataaacgg taatatagca
    15841 aatattataa gcgtaagagg aatatttata tctataagta taaagaatga accaaccaac
    15901 attactatag aaatgaataa atcttcagga ccatgatgtg caagttctgt tatatccatt
    15961 aaatcattta ctattctaga catagttact ccagatttgt tattatcaaa atacgtattt
    16021 ggaagtcttt gtaagtgtgt aaatacctca tttctcatat cagcttgcat atctacacca
    16081 ataacatgtc cccaatactg cataaaatag ttaagccctg cttttgctat aaatataagt
    16141 ataagtgcta ctgcaaaaac tactaacatt cttaagtttt tatttggaac aacgtcatcc
    16201 attatatttc tagttatcat aggataaact aaatcacata aggaaaatag aaaagctgct
    16261 ataaggtcta gtgtaaaaat tttcttatat ggcttataat atttgacaaa ttgttttatc
    16321 ataagtatta aatccctcct ttattagtac ttctatatag tacttctaaa aaatacccat
    16381 aaattagtat aacataacaa taactttaaa tcttttataa acttacaaag taataacaat
    16441 ctttttcata aaattgaaga tttatatcat atagttgtat acttgaaaca gcataaaaag
    16501 agctatatct ctatatttaa aattttataa agatacaact ctatatttaa tactaaattt
    16561 tttttataaa cacaaaacta cttcaatgca tttaatatct ttttatctaa tgctgttgta
    16621 tttatacctg ttatatatcc tgttgccttt attgtaggta tatcataagt tgttggtagt
    16681 atagtagtag atacgattaa atcagcacct tcttgttttg ttgaacattc agcaatttta
    16741 cattgctcaa cttctgcgtg tatattattt tctttaacca atctttctac tctgtcacaa
    16801 actattgttg atgttgcgat tcctgctcca catgctacta atattttttt catatattgt
    16861 cctcctacaa tttctttaat attttataac tcaaaataaa attgataaat ttcttatatc
    16921 tttatttata taaataatta tatcatttta tataagaaga acatcttaaa attattcttt
    16981 taatatgttt tttatttcaa cattgcttat acttaaaatt tcatttttta tacaagactc
    17041 tatgtacttt gatatatttt tataatcatt taaattaaac tgttcaatat tcaatttata
    17101 ttctatatca ctgacaatac ctatcaaatc agacttatta gataaaatat cctcatactt
    17161 accttttcta gttacttcca atcttttaaa tctataattt ttatatcctt ctatgataat
    17221 caagtccata tcttcatata attgaatcat tttatataat ggattcttct cttttagttc
    17281 ttcaatatat gctactcgtt tatctgatat tatacagact tttctcgccc cagattttct
    17341 atgtttataa gtatcttttc cttcctcatc catatcaaaa tcatggcagg tatgtttaat
    17401 aactccaaca cgataccctt ttttatttaa ttcttttatt atgccttcaa tcaacgtagt
    17461 tttacctact cctgaatatg agacaactga caatatatta tacataaaat tgtgtccttt
    17521 cataaaacta tctaataaca atctccacta attgattttc ttttattgtc tcattctttt
    17581 taatcttaat catacaatta cttcttagag ttgcagataa tctagctttt gcatctttct
    17641 tatcatttag ctctacatac aatttacctt cacttgtata tagttttcca cttacaaatc
    17701 tatctttatt tgaaagttta ttaaattctc ctttaaatat agcttttata tgatgtattc
    17761 ccttcttata tgttaaatac tcaataacag gaactatcac attatcaaat accactattg
    17821 ctgcatgtgg attaccagaa agaccaaata atagcttttc tcccaatata cctacataac
    17881 aagctccacc aggtttaatg tttatcttcc aaaataattc tgttgcccct atttttttga
    17941 atacatctgg aattaaatct ttatctccaa cagaaacacc acctgttgta attaaaaaat
    18001 ctacactctg taacatattc tttacctttt tttctaactc caatatatca tcagaacaat
    18061 tgcatattgt atctgcctgt attccaagtt ttaataatct tgaatataag gtatatctat
    18121 tactatcgta tattttacca ttctctaaag tctcatttat atcttgtagt tctgtacctg
    18181 tattcaaaat tccaactttt aaatcatcat aaacagttac tttggatatt ccaagacttg
    18241 ctaatactcc aataccatca tatcctatat tctgattttt attaataatc aattgcccct
    18301 tttttatatc ctcaccctta aatatgtaat tatcgtattc ctttaatttt acatttaaaa
    18361 ttatcatatc acctataacc gttacatctt ctttttttat tacacagttt gcaccctgtg
    18421 gtatctttcc accagtcatt atcttcactg cttcattttt atttaatacc ttattaaata
    18481 cttctcctgc atacaaagtc cctataacct ttaaatttct attttcagaa tttgcattat
    18541 atgcataacc atcatagggt gacctatcaa atggaggact atcaattgtt gaatatatat
    18601 cttctgcaac tactttattt actgcatcta taagatttac tatcttgacc ctgtttattg
    18661 gtaaaacatt tttttcaata atttttatgg catcttctaa ttctatcatt ttaaatactc
    18721 cccaatatat tttttattta agatatttct atgtaatata ttatagatta taaaagttgg
    18781 aaatactttt atcttatatt taattataac taatagttat ctataagtgc agttcaaatt
    18841 ttataaatct aataaaaaaa ctgttcccag ctagaaattt ccttctatct tgaaacagca
    18901 tcatatgctt tagaatattt tattctaata aagttccttt tattattttt acaattttat
    18961 cactttcaat agtttttata gattttattt tgttatccac aatacttatt gtaagtacct
    19021 ctttttttaa tattttgtta tcttctttca atctataact cttaactact ttactacctt
    19081 catgattata tgctgttaaa tcataattcg catctcctac aatttcattt attctagact
    19141 cttcaacacc aacatactct cccaggtcaa tacgtctaaa attttctagt gatacagccg
    19201 atttttcatg gtcacttttt tcaatcacaa cactagattc agcatcaatg actgatatac
    19261 cttgtgatga gaaagaattg gtttcaacat tgacaacttt attttcgtta atatcaacat
    19321 ataaagcact tccatctagt tcatcatttc cagtatattt tggataaact aatgtcatat
    19381 aacttgatgt atttatatct tgcattgtat tcgattcttt taaaacttta ggttttatat
    19441 aataaaatgt attataaggt atacccatat tctttataat gtacttataa tctttattca
    19501 taatttcatt aacatcattt tgaacttttg tcatagtttt ttgctgtgtt gctttacttg
    19561 ctacttgatt tttctcttta gtattttctt tatgacatcc aacaagtata atacctaaaa
    19621 tagttacaca aactaatgac ctttttaact tcataaatca atcctccttt cttaaatttt
    19681 atagtattta tctatatctc aatttacagt tatctctttt ttcaataatt atatttatta
    19741 tattataaaa taattatata atatttattt gaatttaata agattaagaa gctaattgta
    19801 atttttctgt aataaaaaac actttaaagt tgaattttga aaattcaatt tacgatattt
    19861 ttattgtaag taccttacaa tattatacca aattattcct aaacaatcaa aaactccatg
    19921 aatatttaaa ggttttctat tttaaaaatc acctttgact attaatttat caaatatgtt
    19981 ataatagttt tagataattt aaggaggatt aaataatgga ttttaaacaa cttgaagtat
    20041 ttgttgctgt tgcaaaacat cagagttttt caaaggccgc aagagaactt tttttaactc
    20101 aacctactgt aagtgcacac atacagaatt tagagagaga gttggaaact gttcttataa
    20161 atagaagcaa caaagttatt acactcacaa aatctggtga aattctttat gaacatgcta
    20221 tatatatatt gaataattgc aaacgtgcaa tttatgatat aaaagaatat tcaggaaaaa
    20281 tagaaggtat tatagatata gcatgtagtt ccatacctga aacttatatt ttacctgatt
    20341 ttatgaagag tttttctatg agttatccag atgtaaaatt ttccataagc cattatgatt
    20401 ctcaatatgc tatatcagaa attttaaatg aacgaataag ctttggatta gttggttcta
    20461 aaatcaataa tcctcaaata gagtacctag acttattaga tgatgaactt gttcttatca
    20521 ctccttcaga ttttaaaatt gataataaaa ataattgtat cgatatagga gaattggctt
    20581 atctaaattt tattatgaga aaagaagggt caggtacaag aaacttaata ctaaacacac
    20641 ttagtaaaaa taactttcca gtaagtaaat taaatgtaat agctcatgta gaatcaaatg
    20701 aggctattaa ggaaatggtt agattaggtc ttggagtctc ctttatatcg tacatttcag
    20761 ctattgatta tctcaatgca ggtaaaataa aatgttataa aataaagggt gttgatttta
    20821 ctagaaaatt tttctttatt tactctaaaa agaaaacttt ttccccatta gaagataaat
    20881 ttctaaacag attatgtgag tatttcgaaa ttataatata ataactttaa taaagtagtg
    20941 cttaattttg ggtttataac taattaataa gctaattata actatcacat aaatgcttat
    21001 aaattattat gaaccatctt tcttttttat ttaatttagt caaaaaatcc ctcgttcaaa
    21061 tgaacttggg atttttttat ttatactatt ccaatagcag ctaatactat acaaataatc
    21121 ataactgaaa ttaatatcgt agttgtcttt accttttttc ctaataacca atacataata
    21181 ccgaatatca tagctggaag catacatggc attatttggt ctaagtaagt ttgaataggc
    21241 tctgcccatt ctccactacc tacattaaca ctaagtttta acattacatt agatgcagtc
    21301 atacctccaa ttaccataag ccctaacatt gaagctgctt ccataacttt tgaaactata
    21361 cttgaactac tcacatcttt aaagaattga gttccatact taaatccttt atctagacat
    21421 atatagcgta taataaaatg aggaacattt attattaaaa ggaataaaat cggtcccatt
    21481 atgttaccct gagaagctaa agctatacca actccagctg ctataagctt taatgttccc
    21541 cagaaaaatg aatctcctat cccagaaact ggacccatta aacttgattt aacagcattt
    21601 atactattag catcaaattc tttattcttt gcattttctt cttccatagc tccagatata
    21661 cctaccaaaa gagtacttat atgaggagta actgacataa attctaaatg tctttttaaa
    21721 gccttagatt tcttttcctt atcatcttta tacaatctac ggattattgg tgccatcatg
    21781 tatgcatatg ctatattttg catacgttcg tagttccaag atgaatctaa tgtcaatgac
    21841 cttctaaagc atttccaaaa gtctttcctc gttattttac tatcgggtga aattgtatct
    21901 aacttcttag aactcgttgt catctactat tacctccttc tctcctacaa agttaacata
    21961 tattaaagct attgctaatc caaacaatgc aactcctgtt actgttatgt ttaggtaagc
    22021 aaccaatata aatcctaata ataaaaatgc tactgttttc ttattaagca tcatcctagc
    22081 taacatggca aaacctattg ctggaatcac gcctgctgca acatccattc catgagttac
    22141 atatgatggt ataaatgaaa gtattttttc tattactgga acacctaatg aaaatcctat
    22201 acctactatt attgctaata ttaatttatt tcctatacca aataataatt gcacacgttc
    22261 tacgccttca atattcccct cttctgcata acggtcagca atatgagctc cccatgtaag
    22321 tactactgtc attaagaagt tatctataag caatactaga gttgctatag gaactgcaag
    22381 tacaactgct gctgatacat cttgacccgt taatattaca aacgctgtgg caataataga
    22441 accagacaca aaatcaggag gattacttgc cccaacagga aatgcaccca taaatactaa
    22501 ttctaatgtt gaacctatta ctatacctgt atgaagatta cctagtgcta aaccaacaag
    22561 tggtgcaaca actataggtc tattatgcat tgtagaacct acaaaactat gcatatatgc
    22621 aaagaatgca attagagtta tcaaaataac ttgtgtaaat tccattttca tccccctaaa
    22681 attatattaa tttcattaca tttgtagcat catcatcagg aactaattgt acaaatacat
    22741 ttacgccttc tgaatctaac tctttaatca tctttatatc atcttttgat acatgcactg
    22801 ctttagatat attttcacga ttatcatctg attttgttcc accaaggttt atagatttaa
    22861 tagcttttac ctctttagca agtctataaa catcttctac agattcacaa agtatcaata
    22921 agttatattt atcagtaaca cctgagttaa gtgcttttat agaatctgct atgcttttca
    22981 ttactaactt aacattagat ggtttagcca ttttaagccc tgccattctt aattcatctt
    23041 ttaataattc atcacttgct atcaatatac agtctgtgct taaaaacttt gtccatgtaa
    23101 atgctacttg tccatgtata agtctgtggt ctactcttac taatttaatc attataattc
    23161 ctccctaatt tttttgattt catccatgaa tgtttttacg tgttctacac taacattccc
    23221 aaaatctttg taattatctt taaagtaact tcctattatt gccatatctc ctaattcaag
    23281 ctgttttttt gcattttcta atgtaactcc agcagctata accaatggaa agtctcctaa
    23341 atttttacga aacagttcta ttttttccat tgatgtttct tgacctgtag cattttctgt
    23401 aactgctata gcatcacatc ttgtcattcc tatttttaaa tcttcttcaa cgtcattttc
    23461 tgataatact ggctgatatt taaaacgtac gcctccaatt aaatacgctg ggcattttga
    23521 acgttgtaat ttaaaaaatt cttctaaagt agcttcgtct ctaggtttaa catgacctac
    23581 tacagaatca acctgtatat aactagcatt atattttgtt gccagttcaa atcccatagt
    23641 atcaacattt aaacaattta ctccatatgg tattgacaaa ttagcttttg atacatattc
    23701 aagtattctt tctaaatcat aataattacc atagtaattt tctaacatta ttccatctac
    23761 accattttct tcaaagattg taatctcttt tttagctctt tcaaagatat cttctggagt
    23821 atctcctttt aagtgtatca ttgctataat tggtttttta cttttaaata ctgataatat
    23881 tccctttttt ttcataaact aaaatacctc ttcttcttct ttaatttgta atgtcacgct
    23941 ttgtattcct cttctagatt cttccactaa ttgttctata tcttcaactg ttaattcatc
    24001 agaaaacctt gtaagaagtt ctaataccat attcaaattc attccagaaa ataacttaac
    24061 attatgtaga ttagttaatc ttaacattgc tgtacataca cttgctccat ataagtcaga
    24121 taaaattata tattcacatt ctatatcttc tgatattgtt ttttctaatt ctaaagcaaa
    24181 atctgaagca ctttgtccag ggtacaaacc atacgaacta acacaatctg ctaaatctcc
    24241 tactatcatt tttactgcat ttaaaagccc ttttgaaaaa tctccatgag atgctaatat
    24301 tatctttttc atacttcatc ctcctactaa atatagatta tatttctgct gatactgcat
    24361 atttgtactt atcacttctt ataaattgct cattgtaata taagacatca ttattttctg
    24421 tatacaccga ttgtcttact ttcaatagtg gcatattact tgcacattta agatagtcag
    24481 aatattcttt gtttgaatgt tttacttcaa atatattttc tactcgttta ggtaatgtgc
    24541 ttatattatt agatataacc tccattaaag atttatcttc taattcttct tttaataaaa
    24601 acatgtaatc tattctaaaa tagtcaactt caaaaataac aggtacttca tctattaatc
    24661 taagtctttt aatacatatt accttctctt ctagttcaat acttaaagct tcagctactt
    24721 gcttatctgc tttttttatt aaaacagaga ttattttggt acttggtttt gcattaatac
    24781 gatgacaaga attagtaaaa ctatttcctg cacatgttgc ttctattctc tctggtattg
    24841 ttacataagt ccctttacca cgttttttac ataatatatc ttcttttaca agttcattaa
    24901 gtgcatttct cacagttata cgacttatat taaattcttt acataagtct ccttcagatg
    24961 gaagtttatc tcctggttta tatatgccag atgctatttg tttttttatg ttgtcatata
    25021 gttgttgata taaaggaaca aaggaattta cttctaaatc catttctttt ttttgtctct
    25081 tttcatccat tttgctcacc tccctatgta acatttttat aacattatat aacaagttct
    25141 ttattacata ttataacaag atgaaatcgt tgtcaatttt tttttaaata atttttaatt
    25201 tgacagtatt ttcttttatt ttctctagtg tgacaaattg ctatttttac ataaaaataa
    25261 gactgttgta caacagatta aattgtttat acaacagcct ttattcactt attacattta
    25321 tatttttact tataaattta tattaaaaaa tgccttatat cccttataca tttcatatat
    25381 gtctacttta gagacaatct catgtggtgc atgcatattt aaaactccaa caccacagtc
    25441 tataacctct gcaccttgat ttgccaatat atgggctata gttccgcctc ctccttggtc
    25501 tacttttcca agttcagcag tttgccatac tacatttcct ttattaaaaa ttctctttac
    25561 ttctgacata aattcagcat tagcatcatt acatccactc tttcctcttg aacctgtata
    25621 cttagttaaa actactccat ttcccatata tgctgaattt ctcttttcat atgcttctcc
    25681 aaaatttggg tcatatccag cagaaacatc agctgataat accttactat ttgctaaagc
    25741 tcttcttact ttaatatcac aataatctcc ttctaaagca atcagttcag ccacagtatt
    25801 ttcaaaaaac tttgagtgca tacctgtatt ccctttagaa ccaatttctt ctttatctac
    25861 acataatgca actgaagtaa attctggttg ttcagtttct agtatagctt ttacagcagc
    25921 atatgaacat accctatcat catgcccata tcccaacacc atagacctat caaaccctaa
    25981 atctctagcc ttaccagctg gaactacttc aatttcagca cttaataagt cttcttcaac
    26041 tatattgtat ttctcattta aaatctttaa tatatttgct gttataggtt ctttttcttc
    26101 atcttctaaa ggaatattcc caattaatat atttaaagct tctccactaa ttccttctga
    26161 tagtttcttt tgcatttggt ctccagccaa atgaatcaat aaatcagtta cacaaaatac
    26221 tgggtcacta tcttcttccc caatagatat gtccactttt gttccatcat ttaatataac
    26281 tataccatgt aaagcaagtg gaagagctgt ccactggtat ttttttattc caccataata
    26341 atgagtctta aaaaaaccta gattactctc ttgatacagt ggatttggtt ttaaatctaa
    26401 tcttggtgaa tctatatggc ttcctataat cttcatacct ttttctatat aatttttacc
    26461 tattacaaaa agtgctactg ccttctcttt atttataaca taaatcttat caccacaact
    26521 tatgcttcct ttttccatag cttcatctat tgaaatatag ttattttctt tagccaattt
    26581 tataatttct tttactgaaa gtctttcagt ctttgactta cttaaaaact ccatataatt
    26641 gcgagaatat tgcataattt gttcaatagt attttctttt ctttcaaaat cccaagcatt
    26701 cttaaattcg tactttaagt tcatattttc ctcctactaa gttctaattt ttattgtttt
    26761 tcatgtattg ctcatattct tctggtgaaa ctactttgac actatctagt cttgatctaa
    26821 agtgagctct aaagttttct aaatattctt tccttaaaat ttctctttct ttgatttcat
    26881 cagcagaaag gattccctct ttatttttct ttgctaattc attaatccta tctaatttct
    26941 tcttgtcaaa cattttttcc tcctaaaaaa cctctattta tttaataaaa taatttattg
    27001 ctttttatat ctaacttgtt tttatattca caaaaaaact catctatatt atcacttatt
    27061 acagatattc ccttttctag ttcttccatg cttgggcgag ctatatttat cctaaagaat
    27121 ctatcatcca ctagattgtc aaaaaaataa gttcctggaa gcattgacac tcctttgttt
    27181 aacataaaat ttgtaaatgc ttgagatgta tatcctcttg gtaattctaa aaaaaagttt
    27241 attcctccac aagattttct aacttttaat ttaccactca atttgtcatt aatcaagttt
    27301 tttgacaatt tatacttatt agtatatatc ttctctatat gactcaaata attcttccaa
    27361 tcaaaattat tcatatagta atacattgat ttttgaatta aacctggtgt tgatatatct
    27421 gatgaatatt tagcccataa aacttttttt aacaactcat ttggcatttc tacaattcct
    27481 atcctaagac ctggcataag tatttttgaa aagcttttta tatatactac tctattttta
    27541 tcatcataac ttctaattgt tctattgtcc tttgattcaa aaacaaaatc acttataaaa
    27601 tcatcttcta ttatataaaa atcatgttct tctgccaatt ctatcaattt ctttttttta
    27661 tatgttgagt aagatattcc agttggattt tgaaaattag gcataatgta tataagtctt
    27721 ggttttattt tttccaactt taattttaaa atacctatat caattccatc atctaacata
    27781 ggtatagata ttatttttgc acctctactt ttaaacactt caattgctcc attgtaagaa
    27841 ggttcttcca taaaaacaac atctgaataa cttataagtc ctttacaaac tatatctata
    27901 ccttgttgag cacctgatat tatttgaata ttttccttag tagtttttat atttccatca
    27961 cttagatact ctaccatttt ttctcttaat tcttcagagc caagaccttc atcatattca
    28021 aatattgaac taccatcttt agataatgcc atatttacag catttttaaa tgaatcgata
    28081 ggaaacatat cattagatgg attcccattg tcaaaatgaa ttaaatcttc tctttgcaca
    28141 ataatcttat ttttcctgtt atctataccg cttttataag aatttgaact tgatacaaaa
    28201 gtaccactcc ctataatttt atatacatat ccttcatttt ctaaaagctc gtatacctta
    28261 actatagtag tgttatttac ttgtaaaaaa tttgatagtt ttcttattgg aggcaatttt
    28321 tcatgttcta ctaactcttt atttattatc aattctttta tatgattaaa tatctgtata
    28381 tatttagttg attttttctc atttaaatct attctgtatt tttccaaatt tatcaccgct
    28441 cattgtattt atattttatt gatgctttta tatatatttg atattattta gtatgtatat
    28501 ataataccaa aatttttata aaaaggggaa atatttatgt tttacgacta tcacatgcac
    28561 tcaagtttct ctacagacgg aaaatctaca atggaagaaa tggtaaaaaa atctattgaa
    28621 ctcggtttgg aggaaatttg tttcacagac catgttgact atgatgtata tgctgatgat
    28681 tctttttcaa ttgtctacga agattatttt aagagtttag agactcttca aagcaaatac
    28741 aaagataaaa tatcaataaa aaaaggaatt gaatttggag ttcaaactca attaatggat
    28801 acatacaaaa aggaagcaca tcaatatcca ttggacttta taatatgttc tatccatgct
    28861 atagatacaa tggacttata ccttggtaat tattttaaag ataaaactca atatgaagtt
    28921 tatgaaaatt actatcttta tttatataat attgttaaga attataaaga ttactctgtc
    28981 ttaggtcatt tagatttgat taaaaggtat gcaccttatg acactatttt agatgataga
    29041 ctattttctg atataattga agaaactctt aaacaagcta tttatgatgg caaaggtatc
    29101 gagataaata cctcttgtta tagatataat cttccagacc taaccccatc taagtatata
    29161 cttcaaatgt acaaagactt gggaggggaa atcataacca caggttcaga ttcacatcat
    29221 ataagtcaag ttgcatgtga gtttgactac atatattcat tactaaaaaa tatgggattt
    29281 aaatatgtat ctaaatttaa taaattgaaa ccagaattta taaaattata aatttttaca
    29341 cctccttgtg aatatattta aaagataagg aggtgatttt tttgaaagta ccttatttag
    29401 tactatcaac agttatttta tttatatcca attttattgt aagaatattt ggttttttat
    29461 ataaaatatt cttatcaaga gcactaggtg aaacaggttt aggtatctat catatgattt
    29521 ttaacttttt gatgatttgc ttagctgtta caacaacagg tatacctact gcacttagtt
    29581 gtctagttgc aaagagaaaa gctttaaacg atagacacaa tactaatgct ctatttatat
    29641 caactttata tatatcattt ttcgtagcac taattatatc tatagttgca tctttcaata
    29701 gttctttttt atcacttaaa tttttaaaag atgctaaatt aaatctattt atactagctg
    29761 tttgtcctgc tatagttata ataactcttt caaacgtact aagaggatat tactatggca
    29821 taaagaatgt aaaaatccct gctattggac aaatcattga acaaattggt aaaatattat
    29881 ttgttttttt acttgttatg tacataaata ataaatctat gaattgttat atagctctat
    29941 taggtatatc tattggtgag ttgagcaata ttatatttat gcttatttgc ttatggcgag
    30001 attcatcatt tgacaatagg tatattataa gcataaaaga tttttataat tcttctatgg
    30061 aaacattaaa aatgtctatt cccataactt gtaatagaat gtcaaacata cttttgcaat
    30121 ctataagttc tatgatgata ccatcaagat tagcattatc aggaatgact tatcaacaat
    30181 ctttaagtat gtatggtatt gtcagtggga tggtaatgcc ctttatattt ttacctttta
    30241 ctgttggttc agcgcttatt gtaaatttaa taccaactat ttcacaggaa atggctctaa
    30301 gaaagcgtaa gagtgtaatt aaaaaaatca aatactccat acttcttact ttatttgtag
    30361 gcatactatc atcaatattt ttttatttct ttggaaaaga tttatgtata ttagtattta
    30421 aaaatacact tgcaggagaa tatctaaaag ctatgttttt agttccacta tttatgtccc
    30481 taaatcaaac tctatctggc attttacatt caataagaaa agaattagct tcaagtataa
    30541 atactattac tggcatgtta attcaactaa tagctttata tgtatttctt ccaattccag
    30601 gacttaatat ttatgcatac atatacacaa tgacgatagt atcaatcttt acttgtctat
    30661 tacatactat agtgcttttt aaatcactaa aatctatacg ctagttataa aaacgtgaag
    30721 gctaccaatg taaattatac actttggtag ccttccttag atgattttta tttataattt
    30781 aaactgtcta tatatcttac ctatctatct gtggcaactt ggctttccac ttccatgatt
    30841 aggtccacat tgcgaaaaat cacatacttt agagaaagta tcaacaagtc ttggttggat
    30901 tggatttggt gatgctaaaa ctactatttg ttcacttatt aatgtgctaa ctgtttcttg
    30961 taagcaaagt actaaatcta tatcatttgg tataattaat tcatagaaag gttcacaaca
    31021 ttcgttgcat tctaatttta atatagattt tcctatacaa tctacagtta agttagtttg
    31081 atattcttgt cttaaagtaa cagctcttcc gtcagctggc aagcatattg gtgctgaaag
    31141 tgtgttaaaa gtaaattcta cataatcaac tcttctctta catccacctc ctgctctaac
    31201 tgctgggaaa gctaatgcaa cgaactctgt acatccacat ctacctctta gctctagtac
    31261 taaattacga actgctacag ttaatcctct ttgtttataa gctactgatt tgccttttcc
    31321 ttgtctacag cactcagcgt ttctttctcc acaaactgca aattcaaaag ttgtttcaca
    31381 gtttcttcct actatatctc ctaatgggtc taaatcgaaa tcttctaaag ttgtcattcc
    31441 tgtgtctata actattccat cattacttaa gcatattttt tctatagtta cacttgcttg
    31501 acctgcactt ggaattgaac caaatacttc tactacttct gtttcaaaag ttaatggttc
    31561 tccatttgga cctgttgcat ctgtaaatgt ttggaattgc atagtatcaa atattctatt
    31621 agcctctatt gcaaatggca ctgcatttga tacagagaag tttcttggag atatcggatt
    31681 acaacattct ggtccacatt tgttttcaaa acattcatct gaatccattt cacatggttc
    31741 acaatcacat ttacacttgt catggcattc acatttgtca tcacatggat taggtttgca
    31801 tggattgcat gggttaggtt tacatggatt acatgggtta catggattac actcatcatg
    31861 gcactcacac ttattatcag tattacatcc attatgatgg tgtgattttt taaaccctct
    31921 catgtcttct gaatttattt catctgtata cacttcttct tcattcattg tagacattgg
    31981 ctgattcatc attcttcttt tatttttttt ataatcttgc atttttcatt gccctccctg
    32041 taagcgattc ttattaagtc tttggctttt tgaagcctaa ttaatactat gcttttataa
    32101 tcttaattgt tacttgcttt ttgaatttat caataattag gcgtatttag tagaaaaatg
    32161 catattaata aaaaaagtac tactatttga aatagtagca ctttttttgt cccgaaacac
    32221 cggtggctaa gtaaattcac accctaggca ctcccaggtg ggtgacttgc cagtaacctt
    32281 taacgtccaa actaactagg catgttataa tttaccagac acggtctccc gtatttcatt
    32341 tgtaggttga atttaaaaaa gccttatcgt atgtttcagg atttcatatt attattatat
    32401 aataaatttt gaatttttta aagtggtttt atatagtaat tgagataatc ttattctatc
    32461 attacttttt cttccttttt tctttatgta tttagttaat tatcgttttt tcttagctct
    32521 agttttagta tttttttcat tataattgta ttcggattct tgctcattaa tgttgtatag
    32581 ttcctcatcg tatactatat cttcatcatt ttctatattt tcatagcctg tgttcttatc
    32641 ttctactaat tcatcttcat tatcctccat ttcaccttca tctgttaact catcatcatc
    32701 caattctttg tcttcacaat ctaattcttc gtcttcataa tttaactcat cattattgtc
    32761 ttcatcatca ttattactat catcacctct taaaagactt cctaacaatt tgagattatt
    32821 catactacca agattgttta tatttgccaa agagctcaaa ctaaatccac cttcaccaaa
    32881 gtcatctggt aaaaataatt ctgcaaataa atctatcttc tttttcattc ctatagcttt
    32941 ttcaactgaa ttttttcttt tttctgggaa tacatctata atttccttta tcatcatatt
    33001 ctttttatca tcactatcca tttttctgaa aaaatccgtt tcatcttctg tatctaccat
    33061 atctatatta tttaattttt ttattcctct aactaaatct aaaacacttt ctatttttat
    33121 tagtattttt ctttcatctc ttgacatgta ttttttagtt cttgttatta tttccattcc
    33181 catttctaaa tcttcttcac ttaaatccat gagccttaaa cctcttttta tatcatttgc
    33241 atcaaacctt aaattgccat gtgtagactg tgaaagttta tcatttgcat tttttttgtt
    33301 ttttctaact ctattatttc cttttgattt atttttatca tcaaatatag agatattacc
    33361 attactcaat gcaatcactc caatcaaaaa tagtaagtta ttcaagacaa ttccccctta
    33421 tattatataa ttgccctata ttaaagtatg tttttcatat tttaatgttc catttttaaa
    33481 gctcagtgca tatatattat ctataagaat ttttatttat attatatatc actcacaatt
    33541 caccacttca atattatttc tatatataaa tagtatgaat ataattcaat atatatttat
    33601 agaaaggggt ttttttatga aaaaattaga tacagagtca ttagaaaaaa tggctaaaca
    33661 aaaaaatatt gacaaggata aaatagaaaa gatggctgat agttacaaag gaaaatcaga
    33721 aaatgaactt atggaggaac ttataaaaat aggtaagaac ttagatggaa gagatgaagt
    33781 agtatcaaaa ttcaaagcat ttttagatga aaatcaacgc aaaaaactag ataatataat
    33841 ggaaaaaatc tcagatgcag aaaatcaaag agatactaaa ccagctaaaa ctaagaaggc
    33901 taaacctact aaaggtaaat caaattcacc tgcaccaaaa aatacaccag gtggtcaaca
    33961 aaaaaaatct aaaggtttat ttaagaaaac taaaaagtct aaccctcatg agtagataaa
    34021 aataaactat ttaagtaatt agataattat ttttagttaa tctattatta aatttgatat
    34081 tattgacata aaaaatcagg tagtgcctta ggcactacct gattgatttt aatagtttta
    34141 tttttttctt tctgtaacac tttctctatc aacaattcta taaggtaatt ctattttctt
    34201 attttcaatt aattccttat ttattaattt tataaccatt cttattgcta ctgctcccat
    34261 atcgtataga ggttgatata ctgtagtaag tttaggtcta tacattctag ctatcttaac
    34321 atcattaaat cctgcaacag atatgtcttc tggaacctta tatccagcat cacatatagc
    34381 atttatagca cctatggcag cctcatcacc tgttacaaaa gccgcatgag gtattattcc
    34441 atcatctaat agttctttca tactattgta tccactctca taatcagttc caccatactt
    34501 aatcaaactc ttgtctaatt ctatattgtt atttgaaagt gctttctcat aaccagcaag
    34561 tctttccatt tctaaaacag tatcatcttt actcgtcatt ataaaagcta tcttttcatg
    34621 acctttcttt ataagatgct ctgtcatatc aaaagtagct tcttcgttgc tagtacttac
    34681 tgtatatata tcataatttc tagcagtttt acttatatat gtcgctggta ttccacaatt
    34741 ttgtatatag ttgatatgtt cttgttcaac tatccatgaa atcataacta taccttccac
    34801 ttgttttgct ctcaatagat ttatactctt aagttcttgt tccttatcag agtatgtatt
    34861 cgctaaaaga atatcatagt catacatttt agcaacttct tctatcccat ttaacacctc
    34921 attaacaaaa gaatctgaaa cttctggaac tattacccct attaattgac tcttctttgt
    34981 tactaagctt ctagcaagtg gatttggtat atatccagtc tcttttataa cctctaaaac
    35041 tttttgtttg acttcatcag ttacaggttt tgaatcattt ataactctag atacagtaga
    35101 tattgacact cctgcttgtt tagcaacatc ttttatcgtt atattgcctt tcatcttcat
    35161 cctccttatt ttactaaatt tatctaaaac tgaaccaaat ttttgaaggt tcctatttta
    35221 actacattta tataagtttt tattttacca taaaagcaag tacatttaaa tacattatat
    35281 tttatattat accctatatt caacaaattt aataatattc attcttaatt taaatcatat
    35341 taaaaaataa aataaaaaaa tactggtata aaataccagc atttttttat tctaatgatt
    35401 taagtattag cagccgcaac ctacttcacc atcaaagaat ccaccacatg aacaagttat
    35461 tagcaataat attaagaaag gtatccatga cattatgtca atgtctctcc agcaatcttc
    35521 aaaagctaag aataggaaga atagtactat tatccaccat cctcctccac caaagcatcc
    35581 ttcttctcca aaaaatctat ctaacataac aaagccctcc tttttttaat aactgtgttc
    35641 ttattctaag catccaccac agcagcaaag tattaataaa actattagga atggtatcca
    35701 tgccattatg tcaaattctg cccagctatc tccaaaagct aagaatagga agaatagtac
    35761 tattatccac catcctcctc caccaaaaca tccttcttct ccaaagaatc tatctaacat
    35821 tgtacgttac ctcctgttga tattttgttg agcttttcgc tcctaacata tactatgcat
    35881 ttggtttcca ttttgctaat gtatttttac tattttttta aattttttaa tttgaactaa
    35941 aaaaggtaaa accataactt gtataagtta ctggttttac ctatttaata atcaatattt
    36001 tttagcaaca acaaccatca tcgaagttaa ataataacaa tactattaat ataacccata
    36061 tccatatatt gtcatcgcat aataagtctt ctaaaatgtc tgtgcataat agtaagaata
    36121 ctataactat tattatcaac attttattac caaaaaatcc ttcgcatcct ccaaagaaac
    36181 gttccatatt gtcttctaat tttgtcataa ctaatcctcc ttgaatcatt aagagactaa
    36241 gttttgtctc tttaattaaa tatatgtagt aatagacaga taagttactt ctaaaaagtt
    36301 tttattgatt agtttattta tttctttttc accaagttta ttgtatctat caacttcatc
    36361 cagataatct tttttctcct ctgatatagt gtagattaaa ctttctattg attttatttt
    36421 gtactcctta tatctttcta tcaagaagga tgtttttgaa ttttctattc ctttctttac
    36481 atgcattaac cttgatatgt agtcttcttt ttcattagat acataactct ccttttcagc
    36541 tacatcttca aggattaaat tggtctccat tacataatcc tctatacgtt gcatatctaa
    36601 agttattgaa ttataataaa aatttactgc tataagtaaa aacattccta ctgaagttag
    36661 taatagtcct acatatttca tagcatttcc tcctaagtta tatttgttat caataaagtt
    36721 tattcatcaa ataatacttt attactacat ttataaaatt ttattatttt ttatagattc
    36781 catattgaaa aattcttgat attatattaa actaacatta aaattaatta aaactgcttt
    36841 aggaggctta aaatgtttga gaaaataaaa gaaataatag cagaacaatt aggggtagac
    36901 aatttagatg agataactat ggaagcatct ttaatggacg acttagaagc tgattcatta
    36961 gatgctgttg aaattataat ggcccttgaa gatgaatttg gaatagaaat accagacgag
    37021 gaagctgaga actttaaatg tattggtgat atatgtaaat atatcgaaga aaacaaatag
    37081 tatcgttttg tctacaaata taaatagtct gtgtaaactt ctacataata aattatatta
    37141 gagttcgcac agactagatt acatttattt acaatatata aaatacacgc tcttatattt
    37201 aatttatata ctaagtatat aaattacttt tcttcataaa atgtaacatt ctttattatt
    37261 atatctatag ttccatcttg attttgcttt acagtatact tcattgggtc ttcaaaatca
    37321 gttagattcc cttttatgtc aaaaccatta tccgttttta tacttctttt tttaagcttt
    37381 ttctcaaccc attttttatc tatactaaaa ctttcatcaa gacctttttc ttccatatgc
    37441 tctttaaagc tatcctttaa ctcatcatct ttaattgaat tatcaacaaa gtcatttata
    37501 tcaacttcat gtttttcctt cagtgtataa tttaatatac ttcttacatc ttcagcttgc
    37561 tttatatcat tactaagagc attagttatc caattttcag ctgtattttt aaataccttg
    37621 gtcttgtact tatcatcatc tattttttta gcatttaaaa attctgttac aaacttagat
    37681 tctgaccctt ctttttcagc atccttatct aaaagcctta gatgaaactc atcatttata
    37741 ccacttaaac taatcaaagc tccttgcttt tgtcttaaag tctctgggat accaatttca
    37801 tttgaaacca tttgaatatt aaatttatca tccacaaatt caattgaatg agtgtacaac
    37861 tttttataat ctaatttcag tattgctaca ctcttttcat ctttaatagt gtataagcaa
    37921 attgccaaat cacaagattc tagtgcagca ttaattttca taacgtcaaa caagtaagat
    37981 gcaatttctt tagaattttt caaaaaagta ctttcatcat atattatttg ttcacaacag
    38041 tttttaatta aattgtcatt ataatcttta aaaacacctt ttcttaaatc ctcatctttt
    38101 gcaattcttt ttatagcttt ctggaaaaaa ccatctactt cttgattaac tttgccttca
    38161 aagtcattta atattggaac atcactattt ttgtccaaaa catgtattat aaatttatgt
    38221 attatcatat atatcacccc attatcttaa tttcgatatt aactctttca tatcctctgg
    38281 tatatctgct ttaaatttca atttttcttt tgttcttggt tgttcaaact ctagcttgta
    38341 tgcatgaagt gcttgcctat ttattagctc ttcatctaca taaccataca actcatcacc
    38401 tattattcca tgacctatat gagccatatg tactcttatt tgatgtgttc taccagtttc
    38461 taagcttact tctaatacag ttgcattttc tagtctttct attactttat agtgagtaac
    38521 agatgactgt cctctttcat ctattatcct ctttattgaa tcttctgtag gtctataaat
    38581 tggctcatct atagttccct cattttcttt tataattcca tcaactactg ttatatactt
    38641 tttttcaact ttattttcac tcattgctgt agataaagta tgatgtgcat aagcattttt
    38701 tgctactata actaaccctg aagtattcat atctaaccta tttacaaatc ttatttttac
    38761 gttttctttt tgattatcta tatagtaact aataccattt gctattgttt tatcatagtg
    38821 gctttttgtt gggtgaacta ccataaatgg aggtttattc accataatta tatcaaaatc
    38881 atcataaatt atctgtaaat ttaaatcttg aggttcaaaa tttgcttttt cctcgtctat
    38941 ttttacttct attaaatcac cactatacac ctttaaactt ggttttttat atactccatt
    39001 tactaaaaca ctcttttcct tcttcatttt tgataatgac ctaactgaga aatttagctt
    39061 gtccaataaa acttctttta aagtcatttc ttcttcattt gtatacgata taagattata
    39121 tctttgattc tcttttttaa acaaactttt acctactttc ttttatatct tcctgctttt
    39181 tatatcttca aaatcatgat attatatatt ttacactatt tttgtttaat agataataat
    39241 tatttttaac tataaaaacc tgtaagaata acaaatagtt attcatacag gttaaattca
    39301 taattaaata tttaatttgt aaatattata atctaaaact atctcttaac tattaaagta
    39361 gttcccattc caccacctat acaaagtgta gcaagaccag ttttagcatc tcttctcttc
    39421 atttcatata aaagtgtagt aagtattctt gctcctgagc atcctattgg atgtcctata
    39481 gctattgctc caccattaac attaacttta ttcatatcta tatttaagtc tcttattaca
    39541 gctacagatt gggcagcaaa tgcctcatta gcttcaacta aatctatatc ttcaatagtc
    39601 atattagcag cttctaaagc ttttttagtt gctggaactg gtccatatcc cattatttta
    39661 gggtcaacac cagctgttcc ataagaaact atagttgcaa gaggctctat tcctagttct
    39721 tcagcttttt ctttagccat tactactagc atagcagcac catcatttat tcctgatgca
    39781 ttaccagcag taactgttcc atctttttta aatgcaggtc ttaacttagc aagtttttcc
    39841 attgtagtgc caggcttaat atattcatct ttatctacta cagtgtcacc ttttcttcct
    39901 tttataacaa caggaactat ttcttcatca aattttcctt cagcttgagc tttttcagct
    39961 ttattttgac ttgcaagagc taattcatct tgttcttctc tagttatatt ccattgctct
    40021 gctatgtttt cagcagtaat acccatgtga tagttattaa atatgtctga taatccatct
    40081 tttatcattg aatcaacaaa agcagcatca cccattcttg caccatatct cgcacttggt
    40141 actaaataag gagacatact catattttca gctccaccaa ctaacattat atcagcatca
    40201 cctaatgcta taagttgaga tgccattgaa acagatctta atccagaacc acaaactata
    40261 tttatagtca tagctggttt ttctactggt attcctgctc ctaatgctat ttgtcttgct
    40321 atattttgtc caagacctgc tgtaagtact ccccctaaaa gagattcatc tatcatatct
    40381 ggagttatgt tagctctttt tatagcttct ttagctgctg ttacccctaa ctctaccgct
    40441 gaaactgatt taaatgctcc tccaaaactt cctactgctg ttctagctgc actggcaatt
    40501 actacttctc tcatattcat tacccccatt aaattagatt tttcatatat ccgaaattgt
    40561 tttttaaaca attttattta aacttttaat tcccaaattc ttatttatta taatcataga
    40621 atcctatctt agtttttctt cctaattgat tagctctaac cattttagct aaaagtggat
    40681 gaggtctata tttagtatct ccaaattcag tatataaaac gttcattata gctaaaacaa
    40741 catctaatcc gattaaatca cctaatgcta gtggtcccat tggatggttt gctcctaatt
    40801 tcatagcttc atctatttct tctttacttg caacaccatc tgcatatata ccaacagctt
    40861 catttatcat aggtataagt attctattta ctacaaatcc aggagattca gatacatcta
    40921 ctggtacttt attgatactc ttagataatt caaatactgt atcaaaagta acttttgatg
    40981 ttaactgacc acttataact tcaactaatt tcatcatagg aactggatta aagaaatgca
    41041 ttcctataac tttatctggt ctcttagtag aagaagctat ttctgttata gataatgatg
    41101 aagtatttgt tgccaagata gtatcttctt tacataattc atctagtaac ttgaaaacat
    41161 ctttctttat attcatgtct tctacagatg cttctattat taaatccata tcttttaaat
    41221 cttcataatt agtagttgaa ctaacatgac ttaatatttc tgcttttgta gcttcatcca
    41281 tttttccctt agtaactaac ttagttaaat ttttatctaa taaagctaaa catttatcta
    41341 tagcaccttg agttctactc tttaaacata catcatgtcc acaacttgca aaagtttgta
    41401 caataccact tcccatagtt ccactaccta ttacagctaa tttcatagtt ataacctcct
    41461 gttattttag tatttatatt ctgttaacta gattaaaata ctatttaatc tagttaacag
    41521 caaatatatt tttaattaga tttttaccct tttataaagt tagcttctct cttttcaacg
    41581 aaagctgaca ttccttcttt ttggtcttta gttgaaaaac aaagaccaaa taaattagat
    41641 tctatatcta ttccagtatt tatatcagtt tgagcaccaa gttgtattgc ttctttagag
    41701 tatctaactg caagctgagc atttttagct attatcttag ctaatttctc aacttcttct
    41761 attaaaatgt ctggctcaac gactctattt actagcccta ttttttcagc ttcatcagct
    41821 tttataactt gacctgtaaa gattaattct tttgcttttg ccattccaac caatcttgta
    41881 agtctttgag ttcctccata tcctggagtt attccaagag ttacttctgg ctgaccaaat
    41941 ttagctttag cagatgcaat tcttatatca catgccattg caagttcaca tcctccacct
    42001 aaagcaaatc cgtttacagc agctatcact acttttttac tattttctat ttctccaaaa
    42061 gcttttgctc ctaagatact aaaatcttta gcagctacag catctaaatc tttcatgtat
    42121 gcaatatctg ctccagctac aaatgccttt ccttcccctg tcaatattac aacatcaata
    42181 gtttcatcat tattaatatc tacaaatact tcataaagtt cttctaaagt ctttgaattt
    42241 attgcattaa gggctttagg tctattcatt ttcactgtac atatatttcc atctacttca
    42301 acagctacat tctcataaac tttaacatca cttgtactca taataccctc cccaagaaat
    42361 tagttagcta aaacttcacc tttttctttt gcaacactta actgactgat aagttctgga
    42421 agcactttat gaacatctcc aactatacca acatcagcat atttaaatat tggagcttct
    42481 ggatttttat ttatagcaac tataaactca gcatcttcca taccagctat atgttgtatt
    42541 gctccagata taccacatgc tatataaagg tctggtctta cagttttacc agtttgacca
    42601 acttgtcttg ctttatctaa ccaacctgca tctatagtgg cacgagaacc agaaacttct
    42661 ccacctataa tttcagctaa ttcataaagt atgtctaagt tttcttttcc acccattcca
    42721 cgtccagcag aaactaatat cttagcatct tctattttaa cttgtttttt agcttctttt
    42781 attacttgta caacttgaac taatttatca gcatcattaa attctacctt gaaacggtta
    42841 attacagctt ctttagtttc atcaggttca tttttcttca taacccctgg tctaactgta
    42901 gacatttgag gtctgaaatc tttacaaact attgttgcca ttatatttcc accaaaggca
    42961 ggtcttgtca ttaataataa ttttgtatct tcagctactg caagacctgt acagtcagca
    43021 gtaagacctg tatgtattct agcagaaact ctaggcgcta aatctctacc tattgaagtt
    43081 gcaccaaata atacaactat agggtcagct gcttttattg cttcataagc tgcttttgta
    43141 tatggttcag ttgtatacac tgctaaagct tcatcatcta ctactattac ctcatctgca
    43201 ccatagtgtg ctaatgtatc tattaaacct tctaccttac tacctaaaag taatgcagaa
    43261 acttttgtat cataatcttt tgctatttct gtagcctttc ctagtaattc taaagaaaca
    43321 gtttgaatta cattttctct ttgttctatt actactaaaa cgttacccat tctttatccc
    43381 cccttaaatt atatgatata cttctctttt aatttatcta tgataattcc agctgatgtt
    43441 ttcgcatctt cattgtatat tgtaccagct ggtttaactg attttgtaaa tgatttaaat
    43501 acactagttg gagaaccttt aagacctaaa ttagaagggt caacttctat atcttttaca
    43561 gtccatgttt ctactacatc attttcgaaa gcatcatata ttcttccaac tttcatgtat
    43621 cttggtgtgt tcatatcttt aagagttgtt ataaggcatg gcattttaac ttttaagtca
    43681 tggcaacaat cttcaaattg tctttttact aatacatatt caccttcagt ttttatttct
    43741 tcagcatatg ttattgatgg aagatttaaa tgttcagcta tttgaggtcc aacttgtgca
    43801 gtatctccat ctatcgcctg tcttccagct attataatat caaaatctat attttttaat
    43861 gctcctgcta atgctgatga agttgcccaa gtatcagcac ctgcaaatgc tctatctgtt
    43921 aataatatac ctctatctgc acccattgct aaagcttctt ttaaagccat atctgcttga
    43981 ggaggtccca ttgttataac agttacatga gcacccattt cttcttttaa ttttatagct
    44041 tcttctaaac ctgctttatc atcagggttt attatacttg gtactccatc tctaattaaa
    44101 gtacctgtat taggatctag tttaacttct gttgtatctg gaacttgttt tatacaaacg
    44161 actatattca taaatccttc ctccatatct attttaatat cgacctattg tgtatatttt
    44221 aatatttatc tattttaata attttcctga aataaccatt ctttgaactt cactagttcc
    44281 ttcatatatt tcagttatct tagcatctct catcattctt tctactggat agtcacgagt
    44341 gtatccatat cctccatgaa gttgtacagc tttcgtagta acttccatag ctgtttcagc
    44401 tgcaaataat tttgccattg ctgcttctac tccataaggt tttcctaagt ctttatttat
    44461 agctgcttga tatacaaggt gtctagccgc ttgtacttta acttccatat cagctaattg
    44521 gaattgggta ttttggaatt ttgataatgg tctaccaaat tgtactcttt cttttacata
    44581 tttaacagtt tcatcaagag caccttgtgc taaacctaaa gcttgtgctg ctataccaat
    44641 tctaccacca tcaagagtag acattgctat cttaaatcct tgaccttctt ttccaagtaa
    44701 attttcttta ggtattctgc aatcctcaaa tattaattca ctagtagctg aacctcttat
    44761 acccattttc ttttctttaa ctccaaagct aaacccagga gttccttttt caactataaa
    44821 tgctgatatt cctttgttcc ccttagattt atcagtcatt gccattacta catatatgtc
    44881 accagctatt gcatttgtta taaatatttt tgagccatta agtatgtatt catccccgtc
    44941 taaaacagca gttgtttgtt ggccagacgc atctgtacca gcattaggct cagtaagacc
    45001 aaatgctcct aatttatctc cacttgctag tggttttaag aatttttgtt tttgttcttc
    45061 attaccatat tgatatatag gccatgagcc aagagatgta tgagctgata atataactcc
    45121 tgtagtacca caaactctag acaattcttc aactgccatt atatatccta cagtgtctcc
    45181 accttctcca ccatattctt ttggatatgg tatacccatc attcctgctt ttgccatttt
    45241 ttccactgtt tcataaggaa atctttcttc ttcatcaagt tctgttgcta aaggtttaac
    45301 ttcattttca gcgaagctta catatagctc tttaagcatc tgatattttt tagaatttaa
    45361 atccataata gcctcctcag tattttataa atattttatt ttcatttatt aattaaaatc
    45421 tcggcaacat ctataataag gtatttgaat atacgtcgag aaatttataa ccttaatcaa
    45481 actacgctct atatttatat gtgattatac taaccaatta tgacattaat ttaatcataa
    45541 attgttaatt taaaatcaac tatacttaaa aaaaatctcg ctttttggaa ttttgtgaaa
    45601 tttcaagtta gaataagatt tctttcacac tggtcacaga taaaataatt tgtttacgat
    45661 atttatatta gggtatatta aacattaaat attgtctgta catctg
//
LOCUS       DAELXX010000018        44724 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.018, whole genome shotgun sequence.
ACCESSION   DAELXX010000018 DAELXX010000000
VERSION     DAELXX010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 44724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44724
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.018"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(18..779)
                     /locus_tag="KPH29_000976"
     CDS             complement(18..779)
                     /locus_tag="KPH29_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG1466602.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLSYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDAIVIYTRDK"
     gene            998..1474
                     /locus_tag="KPH29_000977"
     CDS             998..1474
                     /locus_tag="KPH29_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBG1466603.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            1572..1724
                     /locus_tag="KPH29_000978"
     CDS             1572..1724
                     /locus_tag="KPH29_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HBG1466604.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(1884..2072)
                     /locus_tag="KPH29_000979"
     CDS             complement(1884..2072)
                     /locus_tag="KPH29_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBG1466605.1"
                     /translation="MAKVCSVCDKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            2433..2783
                     /locus_tag="KPH29_000980"
     CDS             2433..2783
                     /locus_tag="KPH29_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBG1466606.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            2802..4445
                     /locus_tag="KPH29_000981"
     CDS             2802..4445
                     /locus_tag="KPH29_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBG1466607.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFAKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            4526..6583
                     /gene="recG"
                     /locus_tag="KPH29_000982"
     CDS             4526..6583
                     /gene="recG"
                     /locus_tag="KPH29_000982"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBG1466608.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIRNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMERFKNKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            6642..7196
                     /gene="rsmD"
                     /locus_tag="KPH29_000983"
     CDS             6642..7196
                     /gene="rsmD"
                     /locus_tag="KPH29_000983"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBG1466609.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNVKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            7198..7695
                     /gene="coaD"
                     /locus_tag="KPH29_000984"
     CDS             7198..7695
                     /gene="coaD"
                     /locus_tag="KPH29_000984"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBG1466610.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            7699..8223
                     /locus_tag="KPH29_000985"
     CDS             7699..8223
                     /locus_tag="KPH29_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466611.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            8567..10486
                     /locus_tag="KPH29_000986"
     CDS             8567..10486
                     /locus_tag="KPH29_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG1466612.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQERFNVSKRTIYYDILAINEQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENVL
                     EINSISKEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKSVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSILELINKQN"
     gene            10499..10786
                     /locus_tag="KPH29_000987"
     CDS             10499..10786
                     /locus_tag="KPH29_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG1466613.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVDVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
     gene            10919..12178
                     /locus_tag="KPH29_000988"
     CDS             10919..12178
                     /locus_tag="KPH29_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBG1466614.1"
                     /translation="MLDFISNNILRNPSMLIGLIAAFGLVLQKKKASDVIKGSLLAAF
                     GIIIMETGTGMLVSSIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFAGLAI
                     HLMIARFTPIKTIFLTGHMLYWFPFIFVASGVEAGLNGGVLIAFAAVLSALYWSIMPW
                     LLRKYVFDVIGDDSFTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREIS
                     ISGGMAVFLMFIVVGIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQI
                     MPAFQGISEKVVPNALPAFDCPILFNYKPNAVLIGFLVAMLTSTAMILICNYFNVFNL
                     LLIPLVITSFFECGAAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNW
                     ILIFGGNDLSLFGIISKFIGSLFGGIL"
     gene            12181..12921
                     /locus_tag="KPH29_000989"
     CDS             12181..12921
                     /locus_tag="KPH29_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG1466615.1"
                     /translation="MKFKYIDEIRRLIDIIENEEKVNMEKAVQILVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            13244..14554
                     /locus_tag="KPH29_000990"
     CDS             13244..14554
                     /locus_tag="KPH29_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBG1466616.1"
                     /translation="MKKENKMEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSK
                     KDGEKEKLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISA
                     GEGPDVGYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAA
                     NPGVLYYNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFG
                     NLNWNWYPYLWQAGGDIFNDDLKSVKFNDKSGLEAANFLKELQAYVPEDSLSKDSNEM
                     IESVFGPGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLMSS
                     AKNKELAYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPL
                     VVGPHGVEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            14652..15569
                     /locus_tag="KPH29_000991"
     CDS             14652..15569
                     /locus_tag="KPH29_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBG1466617.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVKRDNSDF"
     gene            15585..16394
                     /locus_tag="KPH29_000992"
     CDS             15585..16394
                     /locus_tag="KPH29_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBG1466618.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDAMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            16451..18199
                     /locus_tag="KPH29_000993"
     CDS             16451..18199
                     /locus_tag="KPH29_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HBG1466619.1"
                     /translation="MEISYVLKDCTFKHNKYSVIEAETWKEKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSTISWKGLENRVRLALNVPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLNIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDKSKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYISIADKY
                     KMSKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDENDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVGTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIENLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKGREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     GYDKVKKYVLELLDRV"
     gene            18462..19484
                     /locus_tag="KPH29_000994"
     CDS             18462..19484
                     /locus_tag="KPH29_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HBG1466620.1"
                     /translation="MFGGINIKRIGIIIMFFLSIAFIGFYLAIRGNSDTVKMQRGINI
                     GNALESPKDFPWDVKMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSDNFKIDEEFFKKI
                     DKYVDYALDKDLIVVLDLHHFEEIMKEPRVHKEEFLKIWQQIANRYQKYDKKLVFELL
                     NEPKENLSSQLLNEYIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNVPKDSNIVV
                     SFHYYEPNDFAFQGNIYHNGFEHLSNITWEGTNEQMDYLKKRFDIVENWANKNKVKIF
                     LGEFGVTKEAPEASRRAWIKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDRDILS
                     ALIEKR"
     gene            19489..21795
                     /locus_tag="KPH29_000995"
     CDS             19489..21795
                     /locus_tag="KPH29_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG1466621.1"
                     /translation="MKKNSKKILITLTIITNIVYILWRIFYTVPKEEGMFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPVISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMRILAEKMGVNYITRTEREGAKAGNLNNAMQHTNS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKNKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKIRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKNIYPKEIALEINENIVLECDLCEGKIDERGLLYKVNNIDSIMQNFFLRDEMMDWI
                     LQNKTIPVSNPSEKKEKSIDEFEPMEYI"
     gene            21767..22882
                     /locus_tag="KPH29_000996"
     CDS             21767..22882
                     /locus_tag="KPH29_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466622.1"
                     /translation="MSLNPWNIFRRDEMKTLKVFFCSAKYLILVFIFFINTTFISSAT
                     SDKILDLSFKKIETDLSSRITYEDTGVKIETDSSKSDKERYLYIYQNIKENWSMYNNF
                     YIEIQNKNKSSQKINLSIQSKNMLEFRLKEGSEIFLEDKNIIYSDKIKEGCIEVPGEF
                     EGKIYVNFNSLINEKSNVVLDSSMLSNIVSWGITFIPSDEEHNIVIIKKISLLSEEKL
                     SFLHNIKITGNEEVQIPVLGQSISQYEVLGLKRDSKIKYSLMGKQDNVSISQNGKLTL
                     SNKAEPGQIILQVNVDDKFKIGRKIALTESWSINKKDKDGVPYTLVSPEQSPTVQYMK
                     KINFMNNIITFVRILFVSLVIICFGIYLYWKKCSKTK"
     gene            22900..24318
                     /locus_tag="KPH29_000997"
     CDS             22900..24318
                     /locus_tag="KPH29_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HBG1466623.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCVYNIGVLFIFKYLAFAL
                     RNISELINTELTIPSIVLPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTMQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMLGG
                     NGVIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            24333..25541
                     /locus_tag="KPH29_000998"
     CDS             24333..25541
                     /locus_tag="KPH29_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466624.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNICYSGKDMIQEIKNSVAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSEEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKENYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLDLMDGQSDKYFTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCK"
     gene            complement(25622..27010)
                     /locus_tag="KPH29_000999"
     CDS             complement(25622..27010)
                     /locus_tag="KPH29_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBG1466625.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVTWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IDFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFTIHLIFITLNGLNPITYIRNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSLYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFGKKNIS"
     gene            27352..28686
                     /locus_tag="KPH29_001000"
     CDS             27352..28686
                     /locus_tag="KPH29_001000"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HBG1466626.1"
                     /translation="MRVVIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFDDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPPIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREVSYQGSLSSSCIKIMD
                     MEAASTGITEKQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTSQLGMLDLCYAPPFARTWDVLNVAGNVSK"
     gene            complement(28815..29732)
                     /locus_tag="KPH29_001001"
     CDS             complement(28815..29732)
                     /locus_tag="KPH29_001001"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBG1466627.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLNSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            29940..30824
                     /locus_tag="KPH29_001002"
     CDS             29940..30824
                     /locus_tag="KPH29_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HBG1466628.1"
                     /translation="MKVLKKITILSLITLTLVGCSNGEDKKVVEGSKKIQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRIKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGKKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
     gene            31037..32878
                     /locus_tag="KPH29_001003"
     CDS             31037..32878
                     /locus_tag="KPH29_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HBG1466629.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKNKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVEN
                     IKIENKSVRLIGKEEALKYKLDYVVSKKVDEIKEQQEKILDKVIGATTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFSNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGERITSIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPILKEFGNLESAVIEYIEYKKEISKNVDGRIGIK
                     SKDELISKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSARESSNQGI
                     SKREAKEKSPKTGDLGFSNSIIIFIVSSTLICLLNFNQKELKDKKSK"
     gene            33182..33889
                     /locus_tag="KPH29_001004"
     CDS             33182..33889
                     /locus_tag="KPH29_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1466630.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFAKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
     gene            33883..35193
                     /locus_tag="KPH29_001005"
     CDS             33883..35193
                     /locus_tag="KPH29_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1466631.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIVYSVLSVLIVTLVFILFEYSR
                     LHKLYEHQIKNTQNIAGILIEKYPNDEIDIVNSIYNSDYSKVSTGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMAQE
                     NYLKDTTESEETGYLVEYNEYFKEGTFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEKLEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQVNGEINISVEETANFFKIIIEDNGIGIEKNEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            35310..35993
                     /locus_tag="KPH29_001006"
     CDS             35310..35993
                     /locus_tag="KPH29_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1466632.1"
                     /translation="MKILYTENLSKHYGKGENLVRALDNVNLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDESFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            35986..38373
                     /locus_tag="KPH29_001007"
     CDS             35986..38373
                     /locus_tag="KPH29_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG1466633.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGEMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFELIKGKLPTKENEIVLDSGALKALGYKNKDLGEKIKISYNDYKNDK
                     KIEKEFVISGILKTSEISEAGKYYYAIISELYMRNTRDMSQEDFNIYVKFNDKSNLSI
                     EQAKEKLDKIANDIGLDTINTAVNENYINALKPDMETIMGGGFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQIKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     SFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKSK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKNNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNLIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKAVISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAMYYTSGMMIGSILFGGILGYIAV
                     IFLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            38652..39794
                     /locus_tag="KPH29_001008"
     CDS             38652..39794
                     /locus_tag="KPH29_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBG1466634.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIVGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKEYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLDRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKIPESIGALKLTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNENLLNLFNKYYF"
     gene            39813..40337
                     /locus_tag="KPH29_001009"
     CDS             39813..40337
                     /locus_tag="KPH29_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG1466635.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGSKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLFGYYITEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKKCIK"
     gene            40437..41255
                     /locus_tag="KPH29_001010"
     CDS             40437..41255
                     /locus_tag="KPH29_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG1466636.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYTASNMHFNGIRDKHGFIIQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEAVSELFAKKLEESLNPTQSVEKIVLYLEEMLLSILEEDLISNKSV
                     KLLNSFIYDNRYSNIKAFCDNTNINIKTLERICLKYTGYTPKILKRIYRFKMASSQLI
                     YNYKDNELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFIGENKTIKENIKYNYL"
     gene            41333..41644
                     /locus_tag="KPH29_001011"
     CDS             41333..41644
                     /locus_tag="KPH29_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBG1466637.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSASSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(41947..43950)
                     /locus_tag="KPH29_001012"
     CDS             complement(41947..43950)
                     /locus_tag="KPH29_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HBG1466638.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYEDMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGFFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     NFDLKNITSPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELIKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFSSTPPSSKMPIASRTAIVLLDNGI"
     gene            complement(44103..>44724)
                     /gene="recX"
                     /locus_tag="KPH29_001013"
     CDS             complement(44103..>44724)
                     /gene="recX"
                     /locus_tag="KPH29_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBG1466639.1"
                     /translation="IEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNEINEEEL
                     KPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIIERVIDFLKSYNLVND
                     SVLAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTINEAVSELDKDTEFENAMYLAKK
                     RYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
BASE COUNT        17430 a         4847 c         7662 g        14785 t
ORIGIN      
        1 acctttatta ttttatatta tttatctctt gtatatatta ctatagcatc ccttaaaaac
       61 ttagctaatc caggttgttc tctatcataa tatgatgtaa aacgttcatc atatacatac
      121 atctgtgcta gagaggaatg agcttcttta gagtatgtgt tccaagtgta actcaaccat
      181 ttatgatgta attttgcaac ttcctgagcc agttcactag aggcatcacc agttttaaaa
      241 gcttttttaa gtttactaat tatttcgata tttaggtttt gccaattttc ataatcttgc
      301 ttagacatat ttttaagctt tttatttgat gtatctataa tatctttacc atatttctct
      361 ctaatttcag ttccataatt tttttcattt tcatcaatca tcttttcttt aaaaccttca
      421 aatttttcta tatctgtcat agtgtatttt ccctccttca gagctatagt tttagttaca
      481 ttttctatta gtaaatcaat ttgctttctt ttggctagta atttattatg gtgttctttt
      541 aacgcactta aactatcgaa gtttggtgaa tttataatgt tttttatatt ttctaagtta
      601 attcctaatt ctctataaaa aagtatttgt tgaagtttat taacttcatt ttgaccatat
      661 atacgataac ctgaagaatt tattttaagt ggcttaagaa gacctatttc atcataatat
      721 ctaagtgttc tagtacttat accagctagt ttacttaatt tttgaactgt atattccata
      781 agtaatacct cctataaatt aaatatacac ttttacgcaa cgtaaatgtc aatatattaa
      841 aatataaaaa tttatatctt ttaataaaaa tataaaaaaa tatctttact ggatatttta
      901 cacacaactg tcaatactca ctaatgaaaa ttaaaaaata aaatttatag actatcttta
      961 ctaaaatata gaaggtctat agcctaggag gaactgtatg aatttaaaaa gaagcaatga
     1021 aaaacgagaa gagtacagaa gaatgtattc agataaagaa agttttttaa gtttgattca
     1081 aaattttaca agtgtctcaa tagcaaaaga attaactctt aaaaatatag agttagaaac
     1141 atcttttata tgtgaatata aaggaaaaga agttgatata atttataagg tattttctaa
     1201 aagtggcaaa gtatcacatt atatagtgtt agaatttcaa acagaaatgg atactgaaat
     1261 tgtcccaaga ttgaaatcgt atagagaaca aatttggaaa agttttatta tgaaaaaaag
     1321 ccttgaagaa atagaagata aaaattttaa acttcctaaa gttataccag tggttttata
     1381 tagtggacct gaaaggttaa gtagaaaaaa aggaattttg gatattgtag aatctatatc
     1441 ggagttcagt aataattcta gtatgaaaaa ttaaaattac ataatgtgtt tattggtcta
     1501 aaatggaaaa ctgtcgcata aacttgaata tgaaaatatt tatagataca ggttctatgt
     1561 ggaggtgttc catgagaaat aacaagtcta aggtattgct aggaatactt tgtctagcaa
     1621 caggaatggc tgtaattctg tcaatgttgc ttcctggctg gatttggtca gcacttaccg
     1681 cattaatact tattggatgt ggagcattat tatttttttg ttaaaatagt tttaactata
     1741 aagtgtgggg tgagtattga tgaaagtagt aacaataaaa ttaccaaaat ggatttcaag
     1801 tataatccta aaatttatga gaaagagtaa aaatgattaa attaaaaagc ctacttacgg
     1861 aagtaggctt ttaaacgtgg ttactaagct ctttcaattt taccagaacg taaacatcta
     1921 gtacaaactt ttactctctt aggagctcca tctataatcg ctcttacgct tcttaagtta
     1981 gctgaccatg ttcttttatt atgtttattt gagtgtgata cttggtttcc agaaacctta
     2041 cctttatcac atacgctaca tacttttgcc atctacgcac acctccttaa aatgataact
     2101 atttaaagac taaaaacata tttatattat cataaataac aaaaatttgc aatagaaata
     2161 tatatttgcc ttacacatat tgaagaattt cttattatca tataaaatta tatatataat
     2221 tttatataaa gtaaatatat ttggaaaata attattattt ttctttgaat atatatacta
     2281 tagaagaaat tttatccaaa aacttttcta ggtttaaatt ataccatatt tatagtatga
     2341 tatgattata attttatata ataaataagt aaaaatagta aaaaataaat taagaaatgt
     2401 ttttaataaa aataagctta agggggtcaa ccatgagtgc aaaggtaacg aatcaatatg
     2461 gaaatataga aatagataat caagtaatag ctcaagtcgt atatagagct gcaatggaaa
     2521 gttatggctt agtaggttta gcatataagt ctaaaggaat agtagagcta ttaaaaggcg
     2581 agaacgcaac taaaggtgta agagttgagg aagtagaaga agatgccata gcaatcgaac
     2641 tttatgttat aatacaatat ggtacaaata tatctactgt tgccaataat ataatagata
     2701 gagtaaagta tgtagttgaa aaaatgactg gcgtaagagt tactagaatt gatattaatg
     2761 tacaaggaat aagagttaag taatttcata ggaggaaaca aatgattcaa tatatagatg
     2821 gtaaaaggtt aagggagatg tttatttcag gtgcaaacaa tctccaaaac aataaagagt
     2881 tagttgacaa gttaaatgta ttccctgtac cagatggaga tacaggaact aatatgtcat
     2941 taacaatatc ttatgcctta aaagaattag caaaagttga aaatgataat atatcagata
     3001 tcggaaaagc attatctaaa ggttctttaa tgggagctag aggaaactca ggagttatac
     3061 tttcacaaat aatcagaggt atagctaaat ctatagaagg aaaaagtaaa ttatcaacag
     3121 aagatttagc aaaagctttt aaaaatggtt cagacacagc atataaagct gtaataaaac
     3181 caattgaagg aactatactt actgtagtta gagaaagtgg agagtttgca ataaaaactg
     3241 cgaaaaaaga gaaagatgta gtaaagtttt taagtatgct tgtgaaggaa tcaaattctt
     3301 cattagaaag aactccagac cttctaaaga atctaaaaga agctggtgta gttgactctg
     3361 gtggaaaggg tcttgtactt atatatgaag gaatgttagc ttctataaaa ggcgataaca
     3421 tagaaataaa aaatgttgac ctagatacta atatttcaac aagtatggat tttgcaaaga
     3481 gtactactag cacagatgat ataaaatatt gctattgtac agaatttatt ttagaaagtt
     3541 ctaaggtaga agatactaaa ataagagaca taatgatggc ttatggtgat agtctagcag
     3601 tagttggaga tgatggagtc ataaaggtac atgtacacac taatgaccca ggaaatgtac
     3661 ttcaagaagc tctaaagtac ggtcaattat taactataaa aatagaaaat atgaagttac
     3721 aacatgaaaa tacattactg gatgtagaag aaaagaaaga aaatgatagt gaacctttag
     3781 aagaagagaa agagtttggg tttatagcta cttctatggg tgaaggtttg gcaaatatat
     3841 ttaaagattt cggagttgac catataatag aaggtggtca aactatgaat ccaagtactg
     3901 aagattttat gaatgctata aagcatataa atgctaaaaa tatatttatt ttcccaaata
     3961 acagcaatat aataatggct gctaatcagg caaaagaatt gagtgataaa aatataatag
     4021 ttatacctac aaaaaatacg cctcaaggat ttgctgcatt agtaactttt aatggagaat
     4081 taagcgaaga tgaaaataaa gaagctatga tgaatgcttt aaactcagtt aaatcaggtc
     4141 aagtaacttt tgctgttagg gatacagtga tgaatgaaat tgatgttaaa gaaggtaata
     4201 taattggaat tgcagaagga aatttattat cagcaggaga ttatgtggat gaagtaacat
     4261 caaaccttat tgaaaagttg gttgatgaag atactgctat aataacatta ttctttggtg
     4321 aagatgttac agaatctcaa gcaaatgaac tacgtacttc tttagaagaa aagtttgaag
     4381 atgtagatgt agagttatat tatggaggac aacctcttta ttattactta atttcagttg
     4441 aataatgatt ataagaacct gtatgaccaa gtagctttta taaaattggt tgtacaggat
     4501 ttttgtgtag taggtgatat atttgttgga tttatataaa gatgttcaat atgttaaggg
     4561 aataggacct aaaaaagcag ataaattaaa taaactgggt atatttacac taaaagacct
     4621 attgtattat tttccaagac aatttgaaga tagaaataat ctaaagaaaa tagcacaatt
     4681 agaagatggt gagaaagtta cgataaaagc agtaatatca agtataaata catttagccc
     4741 aaaagaaggt atgactttaa caaagattga tgtaaaagat gagacaggct cggctaaatt
     4801 agtgtttttt aataaaagtt atataaaaaa tacttttcgt ccaggagatt caatattagt
     4861 ttttgggaag gttaagaaaa aatttaataa cttagaactt acatcatgtg aattagaata
     4921 tcttactaat tcacctaaga atacatgtag atttatgcct gtgtaccagc ttacatacgg
     4981 tgttacaaat aaagaaatta tgagtataat tagaacagta cttgaagata aagaattaat
     5041 tatacaagaa tatatgccac aaagaattat agaaaaatat aggctatgta gtatagattt
     5101 tgcagttaga aacatacatt caccaagtag taaggaatca ttaaaaatag ctttatatag
     5161 aattgtattt gaagaattac ttatactaca actagggttg tttgtattta aaagtggcag
     5221 aaataaagaa gatggaataa agtttgaaac aagtaaagac ttaaaaaaaa taataagtgc
     5281 tttaccattt aaactgacta aagctcaaaa cagggcatta gatgaaatta tacaagatat
     5341 gaatttagag aaaataatga atagattggt acaaggtgat gtagggagtg gtaaaacagt
     5401 agtagctctc ttagcacttg caaattgtgt tttaaatggt tatcagggcg ctttgatggc
     5461 acctacagag atattagcag ggcaacatta catttcactt actgagtctc taaaagattt
     5521 tggaataaat gtaggtctat taataggtag tcttacaaaa aaacaaaagg atacagtatt
     5581 ggaacaaata aggaataatg agattgatat attaataggt acacatgcac taattgaaga
     5641 taaggttgaa tttaataata ttggtttagt tataacagat gaacagcata gatttggtgt
     5701 tatgcaaagg agcaaacttt cattgaaagg agcaaatcct gatatattag taatgacagc
     5761 aacaccaata ccaagaactt tagcacttat actctatgga gatttagata tttctattat
     5821 agatgaactt cctccaggaa gacagccaat agaaacaatt gctattgaaa aaagtaagcg
     5881 agatagagca tataataacc ttgtaagaag agaggttgaa tcaggaagac aagtgtacat
     5941 tgtatgtcct ttggtagaag aaagtgaagc tatagaagct aaatcagcag ttgaacttgt
     6001 agaggaatta agagctgaat atttccacga tttaaggctt ggattgcttc atggtaaaat
     6061 gaaatcgagt gaaaaagacg aagtaatgga acgttttaaa aataaagaaa tagatatttt
     6121 agtttcaaca acagttatag aagttggtgt caatgtccca aatgcaacat taatgataat
     6181 agaaaatgca gaaagatttg gacttgcaca attacatcag cttagagggc gagttggaag
     6241 aggtagtcat aaatcgtatt gtgtactaat ttatgattct aaaacagatg tatgtagaca
     6301 aagaatggct ataatggaag agaccaatga tggttttaag atttcagaga aggatttaga
     6361 aataagagga cctggagaat tttttgggac aaggcaacat ggtcttccag aacttaaagt
     6421 agcaaactta tttaaacata taaaaatatt gaagttagca caacaagaag ctcgatatat
     6481 attgggtgaa gataataatc tacaattgaa agaaaacatg gcactcaaaa aagaaattat
     6541 agataaattt aaagatacat tgaaagaaat ttcattaaat taattttaaa ttatgttaaa
     6601 atattactaa tgtaataatg tgctgaaggg agattgtcat attgagagta atttcaggaa
     6661 aagcgagagg attaaaacta aatactccaa aaaatgaaga tgtaaggcca actacagata
     6721 gagtaaaaga gtctctattt aatatgataa attcatatat aatggaaagt gaagttctgg
     6781 atttatttgc tgggactggt tctttaggaa tagagtgctt atctaggggt gccaagtcat
     6841 gtacttttgt agatatcagt aaggaaagca tagatattgt taagtccaat gtaaaaaagg
     6901 caagagttga aagtgaaagt attattttaa accttgattt taagactgca atagataaac
     6961 ttaaattaca aaatagtaaa tttgacataa tttttatgga ccctccgtat tataaaaata
     7021 tgtttataga agctattgaa aaaattgata attctaatct attgaacgaa gatggaataa
     7081 tagttgtaga acatgatacg aatgatttat ttccagataa gatatgtaaa ttggagaaaa
     7141 ctagagataa gaaatatgga aatactacat taacttttta taagatggag gcataatatg
     7201 gaaaataaac ctagaaaagc tatatttgca ggaagttttg acccaattac taatggacat
     7261 ttagatatta tttgcagggc ttcgaagtta tttgacgaat tacaaatagg agtattaaat
     7321 aatcctaata aaaagggtct atttagcttt gatgaaagag taaaacttat agaaaaaagt
     7381 acaagccatt taaataatat aaaagttgtg acttttgatg gtttattaat aaattactgt
     7441 caggaaaatg ggattggagc attagttaga ggtgtgagaa gtggtgctga tgttgattat
     7501 gaacttcaga tggcacatat gaatagagag cttaatccag atatagagac tataatttta
     7561 ccaagttgta caaaatattc atttataagt tcatctttaa taaaagaggt tttgctcttt
     7621 gatgctgata taaaaaattt ggttccaaag attgttttgg aagaattaaa gaaaaaaact
     7681 agtgggggta attgatatat gaagatagat ttagaaatga ttgaactatt tgagcagtta
     7741 gaggagatat ttagaagtgc ttctacaatc cctttctcac ataaatgcac agtggataaa
     7801 gatgaggttt tagcaataat taatgatata agaactttaa taccagaaga agtaacacag
     7861 gcagtatgga ttaataaaga aagaaataaa ataataagtc aagctaagca agatgctaca
     7921 aatatagttg aacaagcgaa aaaagaagct gagagaatac aacaagaata tcaagataat
     7981 atagaggaat taaagaaaaa ttctgaagat gtagtaaagg catatgtaga atccagcgaa
     8041 cctgttgttc aagcagacca aagagcaaaa gatatagttg atagagctga gagaatagcc
     8101 aatgagataa gaattgggtc aattgaatat gcagaggatg tgctttctag tgttgagtat
     8161 aatttaagag aaatattaga agaagtaaaa agagataaag ctgaacttag acctaaaaaa
     8221 taaattttaa ataaataaaa atggagctat cacacaaaaa attccaattg atgtgtgata
     8281 gctctctttt aatatagcaa ttttttgtta tataattgtg ctttttagat tacttatatc
     8341 ttattttgat attataatgt agcttaatct tttatttgtt gaaatcaact tatttatttg
     8401 tataaataat ataggttatt ttcaaatttt tttaataatt ttaccactgt cttcttttag
     8461 attagggtta gactgtgcaa aattataaaa taatactaaa tcatgtgcaa ttatttggta
     8521 tggcactaca tgtagaaaat atagataata aaattaagga ggtagtatga aggaaagggc
     8581 taaaaaaata ttagatttta ttattaaaaa taacaatgta acatcacaag aactacaaga
     8641 aaggtttaat gtttcaaaga gaactattta ttacgatatt ctagctataa atgaacaact
     8701 aggaaaaagt ggtaatataa aaaatgtaaa acacaaattt atatttgaag gtaatttatg
     8761 tgatgcaagg aaaataattt caactgaaga agataaattt ttagatagtg attacagaaa
     8821 aacttttata ttaaacaaga tattacttgg cgaaaaaata tcaatagaaa aactaacaaa
     8881 tgaaatgtta ctttcaaaaa acacagtagt acaaacaata actgatgtaa aaaaatacct
     8941 tcaaacaatg gggttaagat tagagtataa gggaaagtac aaaattatag gtgatgaata
     9001 cgttattaga gagctgtttc tgataattgt tcaagaaaat gtactggaaa ttaattctat
     9061 aagtaaggaa gtaagtagtt tcgatacaaa aggacatata aaacttactg attattcttt
     9121 attaaatcta actaagtttg tggaatttct aaataaaagg ataagagatg ggaaaacatt
     9181 atactcatat aaatatctaa atgaagctaa aaaaattagt tactttagta attgtaaaga
     9241 acttttatgt gaagaagcca atgaaaatga acaagcctat atatgcactt acataagttc
     9301 acttcctagc ttaaatagtg aagttaagga agatgttgtt gaagaatatg tagataaact
     9361 tatagataag tttgaagtaa atacagcaat aaaattagaa agtaagcatg agtttaagaa
     9421 aaatatttta aggcacctcc actcttctta caacagaata cgatttaagt ttcctattag
     9481 aaatccaatg ttagatgaga ctaagtataa acatgagtca ctttataaaa ttataaaatc
     9541 aattatagaa aatgaagaag agtttcctgt atttgagggg ataagagaag aagagatagg
     9601 atttatagca gcgtattttg gagggtattt aagagggtct agggataatg gcctaagaag
     9661 aaataaagtc ttactagttt gtcctaatgg tcttatggta tcaaagagtt tggaaataca
     9721 actatataaa tacataccaa ccatagaaat agtaggtata gtgtccatta agcaactaaa
     9781 agaagtaaat gtatactacg attacataat tacaactata gatatacaaa atgtaaacaa
     9841 tgtaattgtt gtaaatcctc ttttaacatc atcagatgtt caattactta tgaacaaact
     9901 aataagtgta aaagaaaacg agaagtattt caatcttgaa ttaataatac aagctataag
     9961 aaaaaatgga gttatcaata atgaagaagc actaaaagct gatttattaa acataataca
    10021 caagatagat gaaggagaga tgtatcaacc aatgttaaag gaattaatta atgcagaaag
    10081 ggtcaatata ataaaaagtg ttagagattg gaaagaagca ataaaaatag cctcaaagcc
    10141 attgttagaa gataactcta tagaagagtt atatatagaa aatatgataa agtcagttga
    10201 aaaatatgga ccatatattg ttttagctga tagatttgct ctaccacatg caagttctaa
    10261 agaaggagta aacaagcttg ctatgtcttt actaatagta gaagatgaag ttgatttatt
    10321 aggaaaacca gtaaatatat ttatggtatt agctgctgta gataacacta ctcacattag
    10381 agcattagca tcactatcag agatgatgta cgaggaagaa aatgtaaaac taataattaa
    10441 tggtgataaa agttcaatac tagaacttat aaataagcaa aattaggagg aatgaaaaat
    10501 gaagatatta gcagtatgtg gttttggtgt aggttcatca atggtattaa aaatgacaat
    10561 acaaaaggct ctaaagcagt taggtataga atgtgaagta gaaaatacag atataaattc
    10621 agctagaggt gctgattgtg actgtatatt cacaagtcaa gaattgttaa atgaattaaa
    10681 aggaacttgt aatgtagatg tatatccagt taaaaagtac atggatttag cagaagtcac
    10741 agaagttgtt caaacttttt taagtgataa aaatgataag aaatagtata agttttaaaa
    10801 agtaaattta agttaatcag aataaaataa aaacttacta aattcaaaaa tagtaagaaa
    10861 gagaggaggg atttagtggt agaaaactta aacagttgac ctatcactaa aaaatataat
    10921 gcttgatttt atttcaaata atattttacg taatccatct atgttaattg gattaatagc
    10981 agcatttggt cttgtactac agaaaaagaa agcatctgat gttataaagg ggtcactact
    11041 tgcagctttc ggaattataa ttatggagac tggaacaggt atgttagtta gttcaatagc
    11101 accaattaat ggagctttcc aaagtataag tggtggtgca gtaacaaaag gtttaagtga
    11161 tgttactttt actgcaagtt atggtggaat tataggttta gcaatgtttg cgggtttagc
    11221 tattcaccta atgatagcaa gatttactcc tattaagaca atcttcttaa caggtcacat
    11281 gctttattgg ttcccattca tatttgtagc ttcaggagtt gaagctggat taaatggtgg
    11341 agtattaata gcttttgcag cagtattatc agcactctat tggtcaataa tgccttggtt
    11401 attaagaaaa tatgtgtttg atgttatagg agatgattct tttacattag gtcatccatc
    11461 tggttgttta gcacttatat caggatttgt tgctaaatta gtaggaaata aagaaaaatc
    11521 tacagaagat ataaacatac ctcaaagttt atcatttttt agagaaatat ctataagtgg
    11581 tggaatggca gtgtttttaa tgtttatagt tgttgggata gtaataccag gtgcgtttaa
    11641 agatagtaat gagtttattg gaacgtatgc aataaagcaa ggtctttctt ttggagcagg
    11701 tcttataata atgcttcatg gggtaagaat gctaataaat caaataatgc cagcatttca
    11761 aggtatatct gaaaaagttg tgccaaatgc actacctgct tttgactgtc caatattatt
    11821 taattataaa ccaaatgctg tattaatagg attcttagtt gctatgctaa catctacagc
    11881 tatgatttta atatgtaact actttaatgt atttaatttg ctacttatac cacttgttat
    11941 aacttcattc tttgagtgtg gagcagcagc agtaatagga gaatcacaag gtggtatacg
    12001 tggagctata ataggtacag ctatagctgc aatatttatg gtagttttag taggaatatc
    12061 tgctgtaatt tactcaggaa ctattcaaaa ttggatacta atatttggag gtaacgacct
    12121 ttcattattt ggtataatta gtaagtttat aggttcacta tttggaggaa tattatagat
    12181 atgaaattta aatacataga tgagataaga agattaattg atataataga aaacgaagaa
    12241 aaagtcaata tggaaaaagc agtacaaatc cttgttgaag caatagaaag taaaaatgct
    12301 atattttcat ttggagcttc acatgcagga atattaacag aagaattatt ctatagagca
    12361 ggtggctgtg ttcttataaa tccagtattt gcaaaatctc taatgttaga tacagcgcct
    12421 ataactcaca caagtaagat ggaaagatta gaaggatatg gtacagctct agctgataaa
    12481 actccaatta aaaaaggaga tgtagttata gttcattcag tatctggtag aaatccagta
    12541 agtatagagt ttgctatgaa agctaaggaa aataaggcaa tagtaatatg tataactaat
    12601 ttaagttact caaaaagtgt atcttcaaga cattcttcag gaaaaaaact ttatgaagta
    12661 tgcgatttag tgatagataa tcatggtcaa aagggagatg cctgtgtaga gatagatgga
    12721 ttaaaccaaa aagttagtcc aacttctact gtaatagcaa ctactgtgat gaactctatt
    12781 atagctcaag ctactcagga attagttaat aaagggctaa agaatcctcc gattttctat
    12841 agtgccaata ttgatggtgg agatgagtta aataagaaga tttttgatga atataaaagt
    12901 gtaattcatt atgaatatta aggttaaaaa aatatgttaa tgaactttga agtttatcaa
    12961 caaagaatat taataatata taataaatgc tatcatttaa aagctgtagg taaaaatcta
    13021 cagcttttta ttattgtaga ttaaaaagat gcgtaattaa attttaaatt ggagaaaata
    13081 aattggaagg tatcaattat aaatattaga aacgttgtca tagaaaaaaa ctatattaaa
    13141 aaaaagtaca tagaaaaaac tgattagaca ataaatacat gctttgataa tattttaaat
    13201 ggagtgaaaa tgtgtagaat tataatgaaa aaagaaaata aaaatgaaaa aagaaaataa
    13261 aatggaggga ataaaagtgg gtatgttaaa aaaattatgt tcactagcta tggtagcaat
    13321 tatgactaca acacttataa cagggtgttc aagtggaaat ggaaaagatt caaaaaagga
    13381 tggcgaaaaa gaaaaactag ttctttggat gccaccagta gaaaataata tgaaggaagt
    13441 ttgggaccct attttagata aatttgagga aaaaaataac tgtgaagtag atttacagat
    13501 aattccttgg gataattatt ctgaaaaatt tgcaacagct ataagtgctg gcgaaggtcc
    13561 agatgtagga tatatgtatg cagaaatgta tccacagttt atagaaagtg gagcagtaga
    13621 agatttgact aactatgcaa ctaaagagga taaagagcaa tctatttata taaaaacatc
    13681 tgaaatgatg ggtggaatgt atggtatgcc attccaagca gcaaatccag gggtattata
    13741 ctataacaag gatattttag agaaattagg agagaaacct cctaagacat gggaagattt
    13801 taaaagaatt tgtaaaaaag caacacaaga tactgatggt gatggaaaga tagaccaatg
    13861 gggattagct cagggatggg gagctaaaac ttttggtaac ttaaactgga actggtatcc
    13921 ttatttatgg caagctggtg gagatatttt taatgatgat ttaaaatcgg ttaaatttaa
    13981 tgataagagt ggtttagaag cagctaattt cttaaaagaa ttacaagcat atgtgcctga
    14041 agattcttta tctaaagata gtaatgaaat gatagaaagc gtatttggac cagggaaagc
    14101 agcatttaca ataatgttat cttctgctgc aacaagtgta tttgatgaat cattcccgga
    14161 tttaaattgg gggtttgtaa ctggactaga agataagaaa gcagcaactt ttggagcagt
    14221 agaccattta tctttaatgt catctgctaa aaataaagaa cttgcataca agttaataca
    14281 acatatgtta agtgttgaat caatgacaga atttcataaa gcaataccaa gagcaccaat
    14341 aacaaaaggt gaaccatatc aaggtgatga aagattcaaa gaaatggtgg aaaatgacaa
    14401 aaatgtatat agaccattag tagtaggacc tcatggagtt gaaatatatg aatatttatg
    14461 gaaagaactt caaactatga tttcaggaga taaaactcca aaacaagcat tagatgatgc
    14521 tgcaaaatat tcaaatgatt tattagcaca ataaaagttt aaagctgtaa tatgttgtaa
    14581 aggactttac agattaaaat tagcattgta tttataaata caatgctaat atttatattg
    14641 gagaggataa aatggaaaac ttagttagag agtttgaaga aaattcaaaa agaaaaaaaa
    14701 caaaattaaa tattaaacca tatatgtata tattgccatt gggaataatt ttagtggcgt
    14761 tttatgtgat accaataata atgtcaatat actttagttt tacaaagtat aatattatta
    14821 gtccagccac ttttataggt ttagaaaatt ataaaaaatt atttacagat gaaacattaa
    14881 aagtatctat tataaatact attaaattta ctgttgttgt agtaccttgc caaactatat
    14941 tatcattgat tttggcagtt tggattacag gaaaaggaaa tagtaaaata gcttcatttg
    15001 ctaaaggtgc tatatttata ccagtattat catctatggt tttgataggt atggtatgga
    15061 gggctttatt gaatggagaa ggttctatta tatatcaggt attaggtgcc tttggaatag
    15121 aatcatctaa acttttagga gatagtaaga cagcattacc tacacttatg tttatatcta
    15181 tgtggaaaaa tatagggtat ttcatggtaa tttatatttc agctataatg aatttaccaa
    15241 aacactgcta tgaagtagcg aaggttgatg gggctacaaa acttcaagaa tttataaaaa
    15301 taactgtacc tttattgaaa ccaactacaa taatggttgt atttttaggg tcgatatggt
    15361 cattacaagt atttgactta atttatactg tgacaggtgg aggtccaggt atatctacta
    15421 tgagtatagt tatgcatgca tttaacttaa actttaaaaa ctttaattct ggatatgcaa
    15481 tgactgtagc aaacgtatta tttttgttaa tagcagtagt ttcgatttta caaaataagc
    15541 ttgtaaaacg agacaattca gatttttaga aaggtaggta taatatgaaa acagcaagaa
    15601 gattattaag tgatggttta cttttattta tagcgtgtgc aagtttggta ccttttatat
    15661 acatgttgat aatatcattg aagataacat ataactcata tagtttggat atatcttttt
    15721 caacagtaac tcttcaaaac tatatagata tctttactaa aaaaggattt gcacaatatt
    15781 tttttaatac agcaatagta tctttttcag gtgtattatt aaatttagta tttagtacat
    15841 tagcaggata ttcttttgcg aagatggatt ttaaaggtag tgataagtta tttttattta
    15901 tgataatgac tttaattata ccttcccaag ttacaatgat accactatat ataataatga
    15961 aacacttagg atggataaat agttatcttg cacttattat gccaatacca actgcatttg
    16021 gcgtatttat aatgagacaa gctattttag gagtaccaaa agagttatta gaatcagcaa
    16081 agatagatgg ttgctcggat tttagaattt taatacaaat agtattgcct ttaatcaaac
    16141 cagcattaat tacactagca atatttactt ttatgggagc atggaacgag tttatgtggc
    16201 ctcttatagc tacaactaaa gatgcaatga gaacattaac agttggatta tctactctta
    16261 agacattcca gataactaat tatggacaga tgatggcagg ggctacaata acatttttac
    16321 ctccatttat tttctatttg attttacaaa gtaaatttgt tgaaggtgtg tctttgtctg
    16381 gtataaaagg ttaaatagtc taatttaata acataaaaga aacttatata aatatggagg
    16441 aatttaaact atggaaattt catatgtact gaaagattgc acattcaaac acaataaata
    16501 ctcagttata gaagctgaaa cttggaaaga gaaggatttg gatattgtag tagctaaagg
    16561 tgaaaaattt gcatttcaaa ttatgctaaa agcaacagaa gaatttaatt gtacaattga
    16621 taaaagcagt actatatcat ggaaaggact ggaaaataga gtaagattag cattaaatgt
    16681 gcctaatagt ttagaaaata attttagtat aaatatatta ggatatgttc aagatgatac
    16741 aaaggcattt gtgaatgatg ctattttaag ggataaagat gtgttagtag agcaatattt
    16801 gcctcaaaca ttttggattg aaggtcaagt accagaagat tttgaagaaa ataaccttaa
    16861 tataggtatt gatatattta aaagctttgg atatgaagat gaagaaaaag tttgtacaat
    16921 tgatgttcct gtaaaagtaa gaaatgtcgt gctaaagcct ttagataaaa gcaagttctt
    16981 tttagattta tggcaacatc caagttgttt agctagaatg tataaagttg agttatggtc
    17041 tgatatacat tttgaaatag tcgataacta tttaaaagaa ttagcatctt taggagaaaa
    17101 agttgcaaca gttatagttt cagattatcc atgggcaggg caaagttgtt ataaagttta
    17161 taaaaatcca tcaaacctat atgaatataa catggtaagt gtatcaaaag gattggatgg
    17221 aaagattaaa tgtaactttg agagtatgga taggtatata agcatagctg ataagtataa
    17281 aatgtctaag gagatagatt tatttggtct attaggtaat tggtgtgctg gtgagtttgg
    17341 aaatcctgta gaaggttata aggaccctat aagagtaaga tattttgatg aaaatgataa
    17401 agtatttaaa tttataaaca atacaaatga tttaaaagaa tatataggat tagtattaaa
    17461 tcatttaata gagtgtggat tatgggatag agtaaggatt atagctgatg aaccaaataa
    17521 tccagaagta gtcaaagagt gtatagaatt tataaattct actgtaggta ctcatcaagt
    17581 taaatataaa tcagcaactc acgaccaaaa ctttttagat agagctaaag atgaaattga
    17641 tgatatgtca ataaatttaa aacttactat acaaaattat aaagatatag aaaatcttaa
    17701 gaaaaaaatc aatgacaagg gtgggatatt gacttggttt gtatgctgtt ttccagagaa
    17761 accaaatagt tttttaagtt ctccttttgt agaaaataga ataataggat ggtatactta
    17821 ttactttggt cttgatggat ttttaagatg ggactataat ttatggacag aagacccttg
    17881 gaaagattca agttacaaat tccctatttg gaaagctgga gatatgttct ttgtttatcc
    17941 tggaaaagat ttaaagccag ttagaagtgt cagaatggag aatttaagat ttggaataca
    18001 ggactttgag ttatttacaa tgttagaaaa agaaaagggc agagagtata tagaagttga
    18061 gcttatgcaa gaattattaa ataaaaaaga aaatgctgaa attaaaggtt ttggtgacat
    18121 agaattagga tattcacttg ataattgggg atatgataaa gttaaaaaat atgtattaga
    18181 gttgttagac agagtataaa aaaatataag agtgtctcaa aatgaacttt ttagttcatg
    18241 aggcactctt tttgtatgaa atcaatatag tttcattgtt tcaacaatga aaaataggat
    18301 tacttatatt ctaagatagc cctttagttt tttagttgta ttcaatgttt taaaactaac
    18361 tataaacgct ataaagttta taaatgacaa taattatatg taacatgatg tactttaatt
    18421 tgctataata gggattgaat aatattatat ggaagataac attgtttggg gggattaata
    18481 taaaaagaat aggtattatt ataatgtttt ttttaagtat agcttttatt ggattttatc
    18541 ttgcaataag aggtaacagt gatacagtta agatgcaacg agggataaat ataggtaatg
    18601 cactagaatc acctaaggat tttccgtggg atgtaaaaat gtcaaataag ttttttgatg
    18661 atataaaaga tgcaggtttt gacacagtac gtataccagt aaggttttct gattatacgt
    18721 cagattctga taattttaaa attgatgaag aattttttaa aaagattgat aaatatgtag
    18781 attatgcatt ggataaagac ttaatagttg tgttggattt acatcatttt gaagaaatta
    18841 tgaaagaacc aagagttcat aaagaagagt ttttaaaaat atggcaacag atagctaata
    18901 ggtatcaaaa atatgataaa aaattggtat ttgaattatt aaatgaacct aaagaaaatt
    18961 tatcttcaca attactaaac gaatacattg aagaagcaat aaagataatt agaaaaacta
    19021 accctaaaag aactattata gtgggaccat ataatttcta tcaaattgat tatttgaatg
    19081 aattaaatgt tccaaaagat tctaatatag tagtttcttt tcactattac gaaccaaatg
    19141 attttgcatt tcaaggaaac atttatcata atggatttga gcacttgagc aatattactt
    19201 gggaaggaac aaatgaacaa atggattatt taaaaaaaag gtttgatatt gtagaaaatt
    19261 gggcaaataa aaataaggta aaaatattct tgggtgaatt tggagtaaca aaagaagcac
    19321 cagaagcttc aagaagagct tggataaaag ctgtaagaga agaagctgaa aaacgaaact
    19381 tttcatgggc ttattgggag cttgcttctg gatttggtat atacaatcaa attgaaggga
    19441 cttgggatag agacatatta agtgcattaa tagaaaagag gtgattttat gaagaaaaat
    19501 tcaaaaaaga tacttataac tttaactatt attactaata tcgtatatat attgtggaga
    19561 attttttaca cagtacctaa agaagaaggg atgtttgcct taatctgtgc tataatactt
    19621 ctttttgtag agataatggg tatgatggaa atgtttgtac attattatgg tatgtcaaat
    19681 attgagtatc ctgaaaaacc agtcataagt gaagaacttt atccacatgt agatgtattc
    19741 attgcaacat ataatgagtc tgttgatttg gtgagaaaaa ctgtaaatgg atgtattcat
    19801 atgcagtatc cagataagaa aaaggtacat atatacatat gtgatgatgg aaatcgtgaa
    19861 gaaatgcgta ttctagcaga aaaaatgggt gtaaattaca taactagaac tgaaagagaa
    19921 ggagcaaaag ctgggaattt aaataatgca atgcaacata caaactctcc tttgattgct
    19981 acgtttgatg cagatatgat accaatgcat gactttttaa ttgctacagt accatacttt
    20041 ttgaaaaatg agcaagctaa aaaagatgga gaaaaagagg agtatgaaaa ggttggtttt
    20101 gtgcaaactc cacagagttt ttataatcca gacttgttcc aatttaatct gcactctgag
    20161 ggaaggattc caaatgagca agattatttt tacagagata ttcaattggc tagaaatagg
    20221 acaaattctg taatttatgg tggtagtaat acagttattt caagggaagc acttgaagaa
    20281 gttgatggat tttatactta ttctattaca gaagattttg caacaggtat cttaattcaa
    20341 agtaaggggt atagatgtta tgctattcca gaagtacatg catcaggttt atctcctaca
    20401 gatttaaaaa gtcttattaa gcaacgtgaa agatgggcac gtggatgtat acaaactggt
    20461 agacgtctaa atattttgtt taggagaggt ttaggttttt ggcagaaaat aagttatatt
    20521 tcttcaatta cttattggta tgcaagtata aaacgctttg tatatataat ggcacctatt
    20581 ttattttctg tgtttaatgt gattgtagta aaatgtacat tacttcaagt attggtattt
    20641 tggttgccaa tgtatatttt aagtagttta tctcttaaaa tattttctca aaatataaga
    20701 aatactagat ggaccaatat atatgaaact ataatgtttc agtctttaat gccagcagta
    20761 atacttgaaa cgtttgcaat atctaaaaat aaattttcag taacaaataa gaataaatta
    20821 gaagaaaata gaatgtataa gtttttacag ggaatacctt attttattta catggtctta
    20881 tctatcatag gaattttaaa aatgtttgta gcaatattta aaatgagttc aatgacatat
    20941 tcagttgtat tattttggtt gattggaaac ctttttaacc ttgttatggc tacattattt
    21001 atatcaggga gacaacagtt aagaaagtct gagcgttata ttgctgaaat tgattttaag
    21061 ttaaagcaaa actcttatgt tctttcttca aaaacgattg atatatcaga aaatgggttt
    21121 gcatttttgc tagaaaatcc agaatatatt tccccagaag aagagttcga ggtagagttt
    21181 agagaaaaat caggaaatga aatgtatata gctaatatga aagcaaagat tgttaatgtt
    21241 gtggaagtaa attcaaagtg gaaatatgca gcttatatta cacatattga agatagtgaa
    21301 atagataatt ggatgtgtat tgttcacgat agaataccta ctttaccgat gacaatatct
    21361 aatcaactag gtttctttga tgatttgcaa attaatgtaa aaaaacgtat tgaaaagatt
    21421 agaacattat cacgaagaag tccaagaatc aatatgaatt ttcagatgga tataaaaaat
    21481 acaggtaaat taagaatagt aaattttaat tatcaatatg tattattaaa ttttgagaat
    21541 aaaaatattt atcctaaaga aatagcattg gaaattaatg aaaatattgt gttagaatgt
    21601 gatttatgtg agggtaaaat cgatgaaaga ggtctcttat ataaagtaaa taacattgac
    21661 agcataatgc aaaatttctt tttaagagat gaaatgatgg attggatatt acaaaataag
    21721 acaattcctg tttcaaatcc tagtgaaaag aaggaaaaaa gtatagatga gtttgaaccc
    21781 atggaatata tttaggagag atgaaatgaa gactttaaaa gtgttttttt gttctgcaaa
    21841 atatcttatt ttggtgttta ttttttttat aaatactact tttataagtt cagcgacaag
    21901 tgataaaatt ttagatttat cctttaaaaa gatagagact gatttaagtt ctaggattac
    21961 atatgaagat acaggtgtaa aaatagaaac agatagttct aaaagtgata aggaacgtta
    22021 tttatatatt taccaaaata taaaagaaaa ttggagtatg tataataatt tttatattga
    22081 aattcaaaat aaaaataaaa gttcccagaa aataaattta agtatacaaa gtaagaatat
    22141 gcttgaattt aggttaaaag aaggtagtga aatatttttg gaagataaaa atataatata
    22201 ttcagataaa ataaaagaag gatgcataga agtaccaggt gaatttgaag gcaaaattta
    22261 tgtgaacttt aatagcctta taaatgagaa aagtaatgtt gtgctggact caagtatgct
    22321 ttcaaacatt gttagttggg gaattacatt tattccatct gatgaagaac ataatattgt
    22381 tattataaaa aagatatctt tattgtctga agagaaatta agttttttac ataatattaa
    22441 aataactggc aatgaagaag tacaaatacc agtattgggt caaagtataa gtcagtatga
    22501 ggtattaggt cttaaacgtg attctaaaat taagtattct cttatgggaa aacaagataa
    22561 tgtgagcata tctcaaaatg gtaaattaac attaagtaat aaagccgaac ctggacaaat
    22621 tattttacaa gttaatgttg atgataagtt taaaattgga aggaaaatag cattaactga
    22681 atcttggagt attaataaaa aagataagga tggtgttcca tatacattgg tatctcctga
    22741 gcaatcacca acagtacaat atatgaaaaa aataaacttt atgaataaca tcataacgtt
    22801 tgttcgaatt ttatttgtat ctctagtaat aatatgcttt ggaatatatt tatattggaa
    22861 aaaatgttca aaaactaagt aaagagagga ggttagattg tgttattttc aagtttaatt
    22921 tttttatttt attttttacc tataactttg gttttatatt atgtatttag gtttaaccga
    22981 acgatacaga acatgatatt gcttgctgtt agtttatttt tttatgcttg gggagagcca
    23041 aaatttgttg ttatcatgat agtttccata ataatgaact atatatttgg tttactagta
    23101 gatagatata gggaaagtaa aataaaagta aaagtttttt tagttttgat gtgtgtatat
    23161 aatataggtg ttttatttat atttaaatat ctagcttttg cgctaagaaa tattagtgaa
    23221 cttattaata ctgaactaac tatacctagt attgttttac ctataggaat ttcatttttt
    23281 acatttcagg gaatgtctta tgttatagat gtatatcgta gacatggaga agtccaaaag
    23341 aatccttttt atgtgggact gtatatagca tttttcccac aattaatagc aggtcctata
    23401 gttagatatg agagtgtggc agaacaaatt ctcaatagaa aagaaacttg gaataaattt
    23461 tctattggga cttgcaggtt tataacaggt ctaggcaaaa aagtattaat ttcaaataac
    23521 atggctatag tagcagacta tatttacact atgaatagtc aaggagaaat agctgcatca
    23581 cttgcatggc ttggttcaat agcctatact atgcaaatat tctttgattt ttctgcttat
    23641 tcagatatgg ctataggttt aggtcttatg tttggtttta aatttgaaga gaactttaat
    23701 tatccatata tatcaaaatc aattagtgag ttttggagaa gatggcacat ttctttggga
    23761 atgtggttta agagctatat atactttccg cttggaggtt ctagagtagc aaataaagat
    23821 attatgatta gaaatatgtt tatagtgtgg ttatttactg gaatatggca tggagcagaa
    23881 tggacatttg taatttgggg gattttaaac tttgttttct tgattataga gcgatttata
    23941 atgtttgaaa agattgaaaa tcacaatttt ataaaacata tttatgcgtt attagtagtt
    24001 aactttgggt gggtattgtt taggtcacct aatttaaaag aagcatataa ttatttcaaa
    24061 gctatgcttg gaggcaatgg agttatatgg agtgattata catatatgtt tttaaaagaa
    24121 tactgggttt tctttatatt tgcatttatt tttagtgtac ctattgcaaa gaaaatcaat
    24181 aagtttgttg ttgaacaagc taaatatagt atgatattta acgtatttta tccaatctca
    24241 gctattttat tgttctttat ttctgtgaca tatttagtaa ctggaagcta taatccattt
    24301 atttatttta atttttagtt gggaggaaaa gtatgaatag attttttaaa gtaagattaa
    24361 taactgcaat tatgttctta attattttat tcgctttctc tatctttaat atttgttatt
    24421 ctggaaaaga tatgatacag gagataaaaa attcagttgc tgaaaaaagt agtttaaaag
    24481 atacaatatc atctattgat aacacagtaa atgacaaagt aatatataaa aatacgttca
    24541 ttgaaactta tggctactta caaaagttaa tgggtaaaaa tgagttttca aaatttagtg
    24601 ttgtaaaaga tacttctgga aagatgcatt atacatattt tgctaaagct ccaaatccag
    24661 ttggtgtgat agcagatagg gtaagaaaat tttctgagga aatggaaaag caaaacacta
    24721 aattagttta cttaatgact ccagataagt atataaaagg agtgacaaaa tttccaaaag
    24781 gaatacctta ttcttatagc aatgagacag cagataattt tttaaatcaa ctaaaaaagt
    24841 ataatgtgga ctatattgat tttagagaaa atattttaaa gagtggtatt ccaaaagatg
    24901 atttattttt taaaacagac catcactgga aagtagaaac ttctttttgg gcatttggag
    24961 aattagttga gcaacttaat aaaaaataca atatgaattt agatgaaaat cattactata
    25021 gagataaaga aaattataac tcaattgtat atcctaagtc atttcttggt tctatgggaa
    25081 gaaagactgg tatcttatat ggtggtatag atgattttac attaatttat cctaaattta
    25141 agactaatta tacttattat acagattcta aatctcaaaa atttgaatta aaaggaaggt
    25201 ttgaagagag tttaatatta tcatatcctt ttaatgcaga ccttgattta atggatggtc
    25261 aatcagataa gtattttaca tatttattgg ggaatagacc attagttaaa ataaaaaata
    25321 tagaaaatcc agatggatta aaagtgctgt ttgttaaaga ttctctaatt gtaccaacag
    25381 cagcattttt ttcctctgtt tgttcatcta tagatatgat agaccctaga tattatgatg
    25441 gagatatact agaatatgca aaatcccatg actatgactt tgtttttata tctgtatatc
    25501 cccaaaatct tacaaaagaa ttttttccgt tttgtaagta aatttctaaa gaaattttct
    25561 tataaaataa aaaataaatg gctaactagt taaaagttag ccatttatct taagtgttac
    25621 tctaagaaat gtttttttta ccaaatactt ctttatctat ttttccaagt gattttccta
    25681 cagtaattga tgataccata gcaccattaa catttatcat ggtacgtccc atatctagta
    25741 tagggtctat tgcaagtata ccaccaacta atggaaaata agaaccaaga ccaactccag
    25801 agataacaac tgaaacggcc attgtagcag ttcctggaag accagctatt ccaaacgaac
    25861 taattgttat aacaattaac aacataacat aaaggctcat atccatgttt actccagcca
    25921 tattggcaag tgttacagcc attaaagcag gatatatacc tgcacaacca ttcataccca
    25981 tatttgcacc taaacttgct gtaaagcttg atacaccctc atcaactccg tgattttgta
    26041 ctagactttc tatcgttaca ggcaatgttc ctaaactaga acgtgaagta aatgccagaa
    26101 gtaaagcttc agttgaattt cttatataag taatgggatt tagaccattt aaagttataa
    26161 aaattaaatg tatggtaaat aatattgcca ctgatatata aagagctaat ataaactgta
    26221 aaacagatac tattgagcta ataccttgtg aagttattgt atttgcaagc agagcaacca
    26281 cagcataagg cataaattta ataacagtca tagacacact tacaattatt ttgtaagatg
    26341 cttctataaa atctataaat ggttttattg tttcaaagtg ttttttactt aatctcttta
    26401 ttgaggtagc tataaatgta gcaaatatta ttgtagctac aatatttcca tttgccatag
    26461 ccattacagg attagatggt agtaatcctc ttaaagtatc cacaagagag tttatttctt
    26521 taagttcttg attttcaact aaagtaggca tattagctcc tacacctaac ttcattatat
    26581 ttgcaacgat aattccaaca attgcagcta tagcagtagt agcaagaagc atacctaaag
    26641 atttaaatgt cagagttcca agattgttat ctttcatatt tataattact tttataatgg
    26701 ataagaaaac taaaggtact actaacattt ttaataagtc cataaatcca gaaccaaata
    26761 ggctatacca cttgcttact tcttgtagcc aagtgacttg agtggtatca ttaggaaaac
    26821 cagcaaaagc ttgtattatg attcccaata tgagtcctag tactgtagca tatattgttc
    26881 tcaatgaaaa ttttattttt ttcttttcaa attgtctaac aataaagaat gtacctagaa
    26941 gtaatattat gaaaaaaatc gttttaaaat ctgtaatcat aagaaattgt gataaaaatg
    27001 aactttccaa tattttttct ccttttattt aaaaataaat ttttctaagt ttaaatgata
    27061 ccaatatatt aaaaaaatgt caatattata tcacaattta cttaaataga gtaaaataaa
    27121 ttgattttga aaaatgtgat taagtataga aaaatagggt ataataaatg tttaaagaat
    27181 ttgaattgtg gtaagaaaat aaaaaatagc ttttataata tttgatttca aattaaaata
    27241 tgttaaataa ataactaaaa attgttatga aaaatgttta catagaatat atatcaaatg
    27301 aaaagcttaa aataagtgaa gaaattaact agacatcaaa ggagagataa aatgagagta
    27361 gtaataattg gtggtgttgc agctggtatg agtgctgcag caaaattaaa aagaataaaa
    27421 cctgagtatg aagttgttgt ttatgaaaaa actgaaatag tatcttttgg tgcttgtgga
    27481 ttaccatact ttgttggtgg tttttttgat gatgctgatg aattattagc aagaacacca
    27541 gagaaactta gagaagctgg aattgattta aatatattta gagaagtcgt tgaggttgat
    27601 agtgaatcta agaaaataaa agtaaaaaat ataaaaacag atgaaatata tgaagattac
    27661 tatgataaat tgatgatagc aactggagca agaagtatta tgcctcctat aaaaaacata
    27721 aaattgaaga atgtttctac attaaaaagt ttatatgatg gagaatatct aaaaaaactt
    27781 ttaagtaatg aagataataa aagagtgaca ataataggag ctggttttat agggttagaa
    27841 gcagttgagg catgtaagaa attaggtaaa gatgtagatg taattcagtt agaagataga
    27901 atattgccac aagtatttga taaggagatg acggatgtat tagaagaaga aataaataga
    27961 cataatgtta atttgcactt ggatgaaatg gttgtagaac tttgtggaga agataaagta
    28021 gaaaaagtta taacaaacaa aggggaaata gatacagatg tagtaataat tgctactggt
    28081 gtgagaccta ataccgagtt tttgtcaaat actaatataa aaatgctaaa aaatggagct
    28141 atagtagttg atgaatatgg aagaacttct gtagaagata tatactctgc tggagattgt
    28201 gcaactataa agaatatagt aagtaatgaa aatgtatatg ttcctttagc tactggagct
    28261 aataaattag gtaggatagt aggagaaaac ctagcaggaa gagaagtatc ttatcaaggt
    28321 tcactatctt caagttgtat aaaaattatg gatatggagg cagcatcaac tggaattaca
    28381 gaaaagcaag ctaaagattt aggcataaat gtcaaatcta aatttatttc tgattataat
    28441 caaactcatt attatccagg aagaaataaa atatatgtaa aacttattta tgatgcagat
    28501 acaaaagtta ttttaggcgg acaagtggct gggtttaagg atgctgtcca aagagcaaat
    28561 gtattggcag ctgctatctt tggaaaaatg accacaagtc aacttggaat gttagattta
    28621 tgttatgccc caccatttgc aagaacatgg gatgttttaa atgtagctgg aaatgtgtct
    28681 aaataatatt tagattatag ctaatatagt tgacaagcaa ttaagggaat gaatttacct
    28741 aaactatttt tatcatggta aaattcatcc ctaaattttt attccaaatt tttagaaata
    28801 aggattccat taaattattc atcaaaattt aaacgcaagc ttaatctatc ttctccaaga
    28861 atagtatttt caaaaacatt agtagcattt tctaaataca tactaggtat gtctaaagaa
    28921 ggactaaaat gggtcagtaa aagttgtttg acatttccaa gttttgcaag attagcagct
    28981 tctctaaaag tcatatgttt gtttctaaca gcttttgata tatctaaatc atcaccatac
    29041 atggcttcac atataaataa atcactatct tttatgaaat ctggtattga ttctataggt
    29101 cttgtatcgg ttgtaagact tatcttaact ccctttctca attctcctaa gaccatatct
    29161 ggagtgtatt gttttgaatt taaaactata ttttgacctt cttggagttt gttccataat
    29221 attttaggaa cttcgttttt agttgcctta tctatatcaa atttagggtt tcttctaaaa
    29281 ttaaaactgt atcctaaaca ctctatagag tgttctaaac tgattgttga aatttctaaa
    29341 tttcttaaaa ctttattatc taaagaaaaa ttgccttgag ggttttctat aatttttaaa
    29401 gtgtatggta catattctac taaatttctc atagaacgta tacaatcgac tattccaact
    29461 ggaccaacta tagttaaatc acttgtacga ccactattcc caattgttga taaaagtcct
    29521 aaaagtccaa atatatggtc accatgtaaa tgggttatac aaatcaagtc tatatcttta
    29581 aagccacatt tttttagctt catagatacc tgagtacctt caccacagtc aattagtatt
    29641 tttctacctt tatagtttat aaatacagag gatagaaatc tgtttggcat aggtacattt
    29701 ccaccacatc caagtaaagt taaatctatc attttttccc ctttctaaaa ttaacacaaa
    29761 tctgttgttt gtttttatta tgatatccta ttataattat tttaacatta aatataacaa
    29821 taaatgtgta tttgagtcaa gaaatattat caaataaagt ttttatatag caatgtataa
    29881 aaatttggag aacgaaaagt gaaaattata tttaggagta tttaaaggag gaaaatttaa
    29941 tgaaagtatt aaagaaaatt actattttgt cattgattac attaacctta gttggttgta
    30001 gtaacggaga agataaaaaa gtggttgaag gttctaagaa aattcaaaat tctaaaaaag
    30061 atgaacagcc tataatagta cataaagatg caaatatatt gaaagatagg tatgagccac
    30121 caaaaggatt taaaagagtt gatgtagaaa aaggtagttt tgaatcattt cttaggaata
    30181 ctaagctaaa aaattatgga gagaaagtaa agtattatga tggaagaatt aaggaatcaa
    30241 aaggtattta tgatagtgta tttgatgttg atattggcaa tagagatttg catcaatgtg
    30301 cagatgcgat tatgcttatg agagcagaat acttgtatca gaataaacta tatgatgaaa
    30361 taagttttga ttttgttgat ggttttaagg cgaagtattc aaaatgggca caaggatata
    30421 gaatttcagt aaaagatagt ggttcaagct ggtataaagc aactgaatat tcaacatctt
    30481 atgagagttt cagaaagttt atggatatag tgtttgcata ttcaggaacg ttatctcttg
    30541 aaaaagaatt ggagcctgta aaagttgagg atatgaaaat aggagatgtg tttatagttg
    30601 gtggaagtcc aggacatgca agtataatta tggatatggc agaaaatgaa aagactggta
    30661 aaaaggtatt tatgatagcc caaagctata tgcctgcaca acaaacgcaa cttcttatta
    30721 atcgtaaaaa tccaaatttg agtccttggt atagtttaga ttttgaaggt gatttgacaa
    30781 tacctcaatg gacttttaaa agagaccagt tgaaaagatt ttaaaataat aattttatat
    30841 aataaaagaa aaatatagat gtgaaaaaat gtaattagat taaaactaat tggatatgga
    30901 caaaattaaa ttgatagata aatattaaaa attacactaa tcaatcttac tatagtttaa
    30961 tatttatcta aaaagtttta tgtaaaatat aaattttata ttaaagctaa aactaatttg
    31021 aggaggctaa tttatattga agattagaag gtatttaatt ttggtaatta taattctaat
    31081 tacaacaagt cagagtttta tttcttatgg gctagagcga tttgaagaaa taaccatttt
    31141 tcacactaat gatatacatg gaagatatgc tgaaggtgat gaccatatac aaataggaaa
    31201 tctagccaca ttgaaaaaag aaactccaaa ctcaatttta gtagatgcag gagattgttt
    31261 gcatggactt ccaatagtaa atatggatag agggaaaagt gcaattgaat taattaaatg
    31321 tgctggatat gactatatta ctcctggaaa tcatgacttt aattatggaa aagatagact
    31381 gtttgagtta tctaaaagtg ctaatttagg agagaatact ttaaaatttt tatcatcaaa
    31441 tatttttgaa aatggaaaaa aagtttttga tagtaatgat ataaaagaaa tagatggaat
    31501 aaaaatagga ttttttggac tctcaaccca ggaaacaaag aacaaaactg gatttaaaaa
    31561 tgtggaagga ttagagttta aaaatcctat agaaagtgca acagaacaag taaatgaatt
    31621 aaaaggaaaa ggtgccaatg taatagttgc aatatgtcat attggtacaa atagtttaag
    31681 taagccaaat agtatagata tagcaaatga ggttcaagga attgatttga ttattgatgg
    31741 acacagccac actaagtttg aaaatggtaa acaagtgggt gatacattga ttgtaagtgc
    31801 aggacaatat ttagaaaata ttggcgagat aaaactaact ttggatacta atgatgtaga
    31861 aaatataaaa atagaaaata aatcagttag attaattgga aaagaagaag cattaaagta
    31921 taagcttgac tacgttgtaa gtaaaaaggt tgatgaaata aaagaacaac aagaaaaaat
    31981 attagataaa gttataggtg caactacaaa tactctagat ggttcatatg aaaatgtaag
    32041 gacaaaagaa actaatttgg gtaatttaat aagtgacatt ttactagata aaactaaggc
    32101 tgatatttct ttatttaatg gagggaatat tcgagatacg atagaaaaag gcaatataac
    32161 aagaagagat attgtagatg tatttccttt tagcaatact atagttacca aagaacttac
    32221 aggagcacag attaaagatg tattggagca tggagtaaaa ctttatccag aaaaaagttc
    32281 agcctttttg caagtgggag gaatttcata ctactttaat cctaaacaaa aagaaggcga
    32341 aagaataaca agtattcaaa aagaaggaaa gccattagat ttaaataaaa agtatgtagt
    32401 tgcaacgaat gattatatag cctcaggtgg agatgaattt ccttgttttt caaaagagcc
    32461 aatactaaag gaatttggaa atttagagag tgcagtcatt gaatatatag agtacaaaaa
    32521 agaaatatca aaaaatgtag atggaagaat tggtattaaa tctaaagatg agttaataag
    32581 caagaatcat gagagtgatt tgatattaag tgaaaatgaa aaaaatatca atgataatga
    32641 agagaatcta tctaataata taaaaagctc tgatagtgac aaaaataaaa gcttagataa
    32701 aacaagtgct agagaaagtt ctaatcaagg aatatctaaa agagaagcta aagaaaaatc
    32761 tcctaaaaca ggagatttag gtttttcaaa tagtataatt atatttatag tttcaagtac
    32821 attgatatgt ttattgaatt ttaatcaaaa agagttaaag gataaaaagt caaaataaaa
    32881 attaatgagc aaaattagta gatagtaatt taattttaga taatagtaaa cagcaaagga
    32941 aattagttat ataaatacta atttcctttg ttttatgctt taacatgatt atttttaaat
    33001 aaaattgtat tttatgaaaa ataattacac taaatggcat agtattatgg ggatttttag
    33061 tttttattca agtaaatgaa atcatattaa caacttctga tatatttaca aattgataaa
    33121 aaaatgattt tattagtatg atataattta gctagtatat atgatgatgg aggatatagg
    33181 gatgaataat atattacttt tggaagatga caaatcttta aatagaggaa ttagttttaa
    33241 attaaaaaaa gaaggataca atgtttttag tgcatttggt atagaagaag caaaaaatat
    33301 tttcgcaaaa gaagagatat gcttaattat ttccgatata ggtctacctg atggtagtgg
    33361 ttttgacttt tgtgaggaga ttagaaagaa aagtgatgtt tacataataa tgttgacagc
    33421 tcttgatgaa gaggtggata ttgtcacagg atatgattta ggtgcagatg attatatcac
    33481 aaagcctttt agcttgatgg tattaatatc aaaagtgaat gcgcttatga aaagagtaaa
    33541 tacagtaaaa aattatactc tattagtttg tgatgattta ttcttttatt atatagaaaa
    33601 taaattaatt ataagaacag atgataagga agaagagata attttaagta agacagagac
    33661 aaaactatta aaatatttga tggaaaattc tatgcagaca ttgactaaag aacaactgtt
    33721 agaatcttta tgggatagta gtggtaattt tgtagatgat aatacgattg cagtaaatat
    33781 aagaagactt agacaaaagg ttgaaaaaaa tccatctgaa cctaaatata taaaaacagt
    33841 aagaggggtt ggatatatgt ggggagaaag gagtataaaa aaatgctaaa aaatatgaac
    33901 ctcaaagatg gtttaagtgt gaaagatgat tatataatga gaagagttat tgtatattct
    33961 gttttatctg tactaatagt aacccttgta tttatacttt ttgaatatag cagattacat
    34021 aaattgtatg aacatcaaat aaaaaatact caaaatattg ctggaatatt gattgaaaaa
    34081 tatcctaatg atgaaataga tatagtaaat tctatttata attccgatta tagtaaggtg
    34141 tctactggag aaaaggcatt taagaaattt ggctatggat tggaaaataa aatgtcaaat
    34201 gataaaaatt ttagtttata cttaaaatct ttttttaagg aatcattttt tattttttta
    34261 atgatgttta tagtagttat aggtataata tattatttta taagatacat aaataaaaga
    34321 ttgagtaaaa tttattttat agtagataag atggcacaag aaaactactt gaaggacact
    34381 actgagtcag aagaaactgg ttatctagta gaatataatg agtattttaa agaaggtaca
    34441 tttagtaaga taaataactg tctatatgag cttaatagaa gtttaaagat aaagtttatt
    34501 aaattagaaa aagaaaaaga aagtgtaaaa tcattagtaa cagatatttc tcatcaatta
    34561 aaaactcctt tagcatcctt aaaattatac aatacattac ttatagaaga aaaattagaa
    34621 gaagatgaag ccaacgagtt cttattaacc aataaggctt ctataaataa attagaaaat
    34681 ttaataaatt ctttagtgaa catatcaagg ttagaaatta gcatgataaa cataaaaaaa
    34741 gaagataatg atataaaaaa tactattcta aatgcagtaa aaagtgttac ccctaaggct
    34801 aagagcaaaa gtataacatt aaatttaaat gaatttaaca gtagaattat accacatgat
    34861 aaaaagtgga ctgaggagag tatttttaat gtattagata atgctgtaaa gtacacacag
    34921 gtaaatggag aaataaatat atctgtagaa gaaactgcta atttctttaa aattataata
    34981 gaggataatg gcatagggat tgaaaaaaat gagtttaata atatatttaa acgtttttat
    35041 agaggttctg ctgatgaaat tgaatctgta gaaggtagtg gggtaggatt gtatttaagt
    35101 agaaagattt tagaagaaca aggtggaaat attatagttt cttcaaaagt agggataggg
    35161 agtaagtttt ctttgtttct tacaactatg taatattaaa tgaaaggaaa atgtaagcct
    35221 ctacatttat tatataagta taaacagctc cagaaataaa aaattaataa aatatggggt
    35281 aatataactt agaatgaggg gataatttta tgaaaatact atatactgaa aatttatcaa
    35341 agcattatgg aaaaggagaa aacttagtaa gagctttgga caatgttaac ttagaaataa
    35401 atgaaggtga atttgttgca ataataggaa aatcaggttc tggaaaaagt acacttttac
    35461 atatgattgg aggtcttgat atacctacat ctgggaaggt gtatatagat aataaaaata
    35521 tatttacatt aaaagaagaa gaactagctg tgtttagaag aagaaaaata gggtttatat
    35581 ttcaaagcta taatttaatt ccatctctta atgtatggga aaatgtagtg ttgccaatcg
    35641 gtctagatgg tagagaagta gatgaatctt ttataaaaga acttttgaaa tcattaggac
    35701 ttgaaaataa acatgacgtt ttaccaaata cattatcagg tggtcaacaa caaagggtgg
    35761 caatagcaag agcattagct acaagaccag ctataatact tgctgatgaa ccaactggaa
    35821 acctagattc taaaacatct gatgaggtta tgagtatatt aaaatctatg tctaagaagt
    35881 acagtcaaac tctagttatg ataactcatg atgacagtat agcccaaatg gctgatagag
    35941 ttatattcat agaagatggc agggtgtcaa aggttggtga taaaaatgat taatactact
    36001 aaaatagcaa aaagtaattt aaaacaaaat aaatcaaaaa gtattctaat aataatcact
    36061 ataatacttt ctactttaat gctttcttct attggaattt atattgttga tgcaggagca
    36121 tatcaaaaag aaaatactat aaagtattca ggtaattatc aaggtatatt agcaaatgta
    36181 gatgaaaaac aggctgatat attgagtaat catgcagatg ttgaattaac tggtgaaatg
    36241 aatggagtag gtgttgaaaa actggaagat gattcaaata tttctctagc atacatgaat
    36301 gaagatgctt tgaaattaaa tagttttgaa cttatcaaag gaaaattgcc aacaaaagag
    36361 aatgaaattg tgttagatag tggagcttta aaggcacttg gttataaaaa taaagactta
    36421 ggtgaaaaga taaaaatatc ttataatgac tataaaaatg ataaaaaaat tgaaaaagag
    36481 ttcgttataa gtggaatctt aaaaactagc gaaatatctg aagcaggtaa atattattat
    36541 gctattatat cagaattata tatgagaaat actagagata tgtcacaaga agattttaat
    36601 atttatgtaa aatttaatga taaaagtaat ttatcaatag agcaagcaaa agaaaaatta
    36661 gataaaattg ctaatgatat aggacttgat actattaata ctgctgtaaa tgaaaattat
    36721 ataaatgctt taaaaccaga tatggaaacg attatgggtg gaggttttgt aggattagta
    36781 attgtattat ctagtatttt agtaatatac aatatatttt atatttcaat agttacaaaa
    36841 gttcaagaat ttggaaaact tagagcaatt ggtgcaacta agaaacaaat aaaaaatata
    36901 gtgtttaagg aagggtttat attagccgga atagcaatac caataggaat aattttaggc
    36961 tatgtacttg caaatattat aataaaatca tttatggata tagatgtaaa atcatcacga
    37021 ttaccagtta tattatcggt tgtggtaata agctttatat ctgttgtttt atcactttta
    37081 aaaccaatga aagtagcatc aaaagtatcc attgttgacg cagtaagata tacaggaaat
    37141 aaaataagta ataagagtaa gagaaaagga tataaaaata taaatctaaa gagattatca
    37201 catgccaatt tagaaagaaa taaaaaacgt acttacatga cattggcatc acttatactt
    37261 agtggaacta tatttataac agtatcaact gctttagaaa gttttgatgc tgagaaaatg
    37321 gcaagagaac actttcctta tgatatagaa gttagactat ctggttatga aatgaacagt
    37381 gataaaaatc ctaaaaataa tttgaatatt ttacaaatgg ataatcctct aagtaaagat
    37441 ttttttaatc aaataaagaa catggaagga attaaaagaa tagaatctgc aagaagtgta
    37501 aaaattggta tggaggacta tgatgttgaa tttaagtatg accttttaca aagtataaat
    37561 gaaaatgatg taaaatcatt aaacaagaat ctaatagatg gaaaaataaa tcttgaaaga
    37621 ctgcaaactg gagatgaaat agtaattact catgttgata cagcaaaaga aatgggagta
    37681 aaagcaggag ataaaataag attaacttta tatgatggag ataaaaagat aaaaaaagaa
    37741 tttaaagttc aagcaatagc tatgggggtt ccaagttttg gtatagggaa ggattttatt
    37801 gatagaactt taaaatacga ttcaactagt agcttgggaa tatatacaga agaaggaaag
    37861 tatcaagaaa tcaaagatag tatcaagaag attgctaaaa ataatggatt tttagagact
    37921 gattttatag atagtagaat tgagtcaaat aaggctgtta tttcatttat aaagataatg
    37981 ggatacacgc taacaggcat aataggtgtg attggattta tgaatcttgt caacacaatg
    38041 ataactagca tagttactag aaaaaaagaa cttggtatgc ttcaagctat tggtcttact
    38101 aataaacaat tagtgaaaat gcttaattca gaggcaatgt attacacttc tggaatgatg
    38161 ataggaagta ttttatttgg aggtatatta gggtatattg ctgttatatt tctaaaaaag
    38221 actggattat cttatgcaac atattctttg ccaatagtac caatcttgtt gatgattgtg
    38281 tgtattctta tagctcaatt tataacaact tatttgatag gacgaagttt taataaagaa
    38341 tctcttattg atagagtaag atacagtgaa taaaatgcaa atatttgtgg ttagtttaac
    38401 tagcaaaaag catataaaaa atatagttaa agagattcat aattaaatag gttaaagcat
    38461 agtacagagc aaacaattta aacggatgtt tgctctgtat tttattatgt aatcaataat
    38521 atatctaata tactgatatt attcatgtta gaaaatgtat cggtacaatt ctaaaaaagt
    38581 ataaaattgt accatataaa atgaaaattt tgtataataa tcgtatagaa aataaatagg
    38641 agctgatata tatgaataag aagattaatt atgcaccagg accaactgaa actagggaaa
    38701 atgtaagatt agcaagagca gaaaaaacaa caaatccaga tatagatatt gattttgttg
    38761 aattttataa aaagacatgc gagaagtttg gcaatatagt tggaacaaaa aacgatgttt
    38821 acatattgag tggtgaagga atattagggc ttgaagcagc atgtgcatca cttaccgaaa
    38881 aaggagatag agtacttgta attgacaatg gtatatatgg agaaggtttt aaagattttg
    38941 taacaatgta tggtggggag tatgtgcttt tttcaagtga atatactaaa agtatagata
    39001 ttgatgaatt gaaagcattt ttagatgagg atagtaattt taaatatgca acagtagtgc
    39061 attgtgatac tccaactggc gtattaaatg atgtaagcaa aatatgtcca ttacttaaag
    39121 aatatggaat attaactgta gttgattctg tagcaggtat ggtaggagaa agattaagtg
    39181 tggatgaatc taaaatagat ataatattag gtggttcaca aaaagctatt tctgcaccag
    39241 caggacttac tatagttgga ataagtcaag atgcaaaaaa ttgtataaaa aatagaaaaa
    39301 ctgatgtaat aggtttttat tgtaatttaa atatatggga aggctattat gaaaaaaaat
    39361 acttccctta tacaatgcca ataagtgaca taatggggct tgataaagca cttgataata
    39421 tattagaaga aggaatagaa aatgtattag atagacatga aaagatagct tatagtgtta
    39481 gaaaatctat tgaggaatat ggtctagaac tatttttaga agaaggatat tctaatacag
    39541 tcacagctat aaaaattcct gaaagtatag gagctttaaa acttactgat tatatgctta
    39601 aaaattataa tacacttgtt gcaacatcac taaatcaata tatggatact attttaagaa
    39661 taggtcatat gggagagaat gcaaatctaa ataaaataga gcatgtacta aatgtcttgg
    39721 ataaaagttt aagtgcatta ggattcaaag gaaatgaaaa tttgttgaat ttatttaata
    39781 agtattattt ttaatagata gggggatata gtatggaagt tacagctaag caaaaattta
    39841 caacgaaaga catggtggaa acctcactat taatagcatt agtatttatt gcaactaaat
    39901 ttataaatat aagattacca atatctataa atggtgggct tgtgcattta ggaacagcaa
    39961 tgttgttcat atcagcaata gtatttggaa gtaaaaaagg tgctttatca ggagctattg
    40021 gtatgtcact ttttgattta atatctgggt ggactttatg ggcaccgttt acctttatag
    40081 ttagaggagt tatgggttat ttacttggaa agattgcatg ggcaaatggt aataatggga
    40141 ataatttttt aatcaatgta ataggaatat gtgtctcatc aatttggatg ttatttggat
    40201 attatataac agaagttata ctttatggta attttatagt acctcttact tcaataccag
    40261 gtaacttaat gcaagtattg ataggtctta taatagcgct tcctatttct aaggtattga
    40321 aaaaatgtat aaaataaata taaagtgaac taaaatttat gatagcataa attatactgt
    40381 ataatattaa gatggagtac ataattaaaa aattttagta tagaggttag ttcacaatgt
    40441 tatcagttac aagatattca atcaaaaatg ttcgtttaaa gccgtgggta aagtttattt
    40501 ggtattttga aacaaaatca aatatactgt taaataataa gttacttcct acagacagta
    40561 tagatattat tcttaatctt tctgatgtta tggaatataa aattgaaaat caagactata
    40621 ctgctagtaa tatgcatttt aatggaataa gggataaaca tggatttatt attcaacatg
    40681 gaaatatacg tgttataggg atttcttttt atccatttgg tctctatcca tttttaaaga
    40741 taccaatttc agagtttaac agacaaattg ttgacttaga agcagtatca gaactatttg
    40801 ccaaaaaatt ggaagagtca ttaaatccaa cacaatcagt agaaaaaatt gttttatatt
    40861 tagaagaaat gttattatcc atattagaag aagatttaat ttcaaataaa tctgtaaagc
    40921 ttctaaattc atttatatat gataatagat atagcaatat aaaagcattt tgtgataata
    40981 caaatataaa tattaaaaca ttggaaagaa tctgtttaaa atatacaggg tatacaccta
    41041 agattttaaa gagaatttac agatttaaaa tggcaagtag tcaacttatt tataattata
    41101 aagataatga actttttgat tttatttatg aaaatgagta ctatgaccaa gcacacttta
    41161 taaaagaatt taaaaaattt tcagggacgt caccaattaa atttattggt gagaataaga
    41221 cgattaaaga aaatataaag tacaactatt tataattata ctgtcgattt tttacaatac
    41281 ctccaattta ttttttgata tgatatagga aaataaattg gaggttttac atatgaaaat
    41341 tattacagaa atagttgagt ttaatgtgga agaaaattta tcaaaagaag agtttataga
    41401 aattgtaaat gacttagaat taaaatttca ttctattcaa tctggtttta ttgatactga
    41461 gcttttatat gatgaaaagt ctcaaaagtg gattatgata caacattggg aatctataga
    41521 agacctcaag agtgcttcta gtaaaatgtt taaagattct tcaactgaga aatttagaaa
    41581 agctttaaag aatcaaactg ttaagatgaa tataatacca caaattaaaa gttggaaatt
    41641 atagaactga atgtaaaaga tagttaaata gtatcaaact aaattagcca aataacattt
    41701 tgtatttaaa ttgttatttg gcttttagtg tttactttaa tatgagtgtt atatactaga
    41761 aaaaccaaca aactgttaga gtgtggatta taataatgtc atatttggga gctaaaaggc
    41821 ttaatactga tttgtcaata tgtcttttta gcatgttgaa caggtatatt gccatgcttt
    41881 cattatttta tttttttgcc ttatactgga ctacatttgc agtttcctaa tatatttggc
    41941 tcatatttaa atgccattat ctaaaagtac tattgctgta cgagaggcaa taggcatttt
    42001 agaagaagga ggagttgagc taaagtatac atcataatct cttaagtttt ctttttttgt
    42061 gtttcctcca aactctacca tatcagtatt taatatcgtt ttgtagtttg tattttgaaa
    42121 ctttgaaata tcaactgatt gtattagatt agggtggaaa ttaaatataa ataaatattt
    42181 gcccttactg tacaccaaaa tctttttatc attatccata tgtataagtt catgactatt
    42241 ttggataaaa atatcactgt attttgtaag ttcgagcata gatttatcaa aatttaataa
    42301 ttgtttatac tttaagttgt tatcttctga aaggctccat tgtcttctgg catacttata
    42361 gctccaattg ttgccttctc gtggaaaatc aatccactca ggatgtccaa attcatttcc
    42421 cataaaattt agatatcctt cacctgctag agaaaaagtt attaatttaa ttaatttaat
    42481 aagagatatt gctctattga taacatggtt atttgagttt atttccatat tccagtacat
    42541 ttccttgtca gctagccaaa aaataattgt tttatcgcca actaatgctt ggtcatggga
    42601 ttcacaatat cctatatttt tttctccagg tcgtctagta gtaagttcat accacatttt
    42661 tcccaaatcc cagtcttcat cagataagtt agagatagtt tttatccaaa aatcaggtac
    42721 tcccattgcg agtctatagt caaatccaat tccgccgtct tttataggga tacacatacc
    42781 aggcattcca ctcatatctt ctgcaatact tatcgaatta ggtttaattt cttttattaa
    42841 ttcatttgca aattggagat atgttatagc ttcaatatct gtattcatac taaaatattt
    42901 ttcataacta tcaaatgaaa ctccaagacc atggttatga tataacattg aagtcacacc
    42961 atcaaaccta aagccatcaa aatggtactc atttaaccaa aattttatat ttgaaagcaa
    43021 aaaatgtatt acttcaggtt taccatagtt aaaaagtttt gtaccccaag caggatgatt
    43081 tccctttgaa cctgagtgga aaaattgatg ttcacttcca tcaaactcat ttattccttc
    43141 taaagtattc tttacagcat gagaatgaac taaatccaat aaaacagcaa tccccattga
    43201 atgagcagtg tttatgaggt tttttaagtc ttcaggagta ccaaacctag atgagattgc
    43261 ataaaagttt gaaacttggt atccaaaaga tgcataatat ggatgttcca taatagccat
    43321 aagctgaatg gtattgtatc cagctttttt tatcttagga agtatttttt cagtaaattc
    43381 attataagtt ccaatagatt cgctttcagt agacatacct atatgacatt catagattag
    43441 tggagaagtt atgtttttta agtcaaaatt attgtcagtc cagataaatg gagtttttgg
    43501 ttgccatatt tgtccattaa atccaccaaa atctttttga attactcttt ttatatataa
    43561 tggtatacgg tcaaatgttt ttccatttgc agtaacttgt acctttacct ctgatttgtg
    43621 aggtaagcta ttttgaccag gaataaatat ttcccaatta ccagacccaa tttttcttag
    43681 aggatgagat tttctattcc aattattaaa gtcaccaatt agtgacaaac tatctgcatt
    43741 tggagcccat tccctataga agaatccatc atttgtctga tgaaatccat agtaaagatg
    43801 agcatttgca aaagaactaa aatctttata gtttttcaat gtttttgatt tcatatcctc
    43861 ataatgtttc attcgctttt ttatatcaga ttcaaatggt tgtaggtata catcaatatc
    43921 taaaatttta aatctttttt gacttttcat tgtatttctc ctccataata aatattacat
    43981 ttaaaaaata atatttatct catactatct tctctgtata attgatatat attatataat
    44041 cctaataaaa agcttaagcc accaatgatt aatttcataa tttacctccc tagtatttat
    44101 acttatatat cgtattcatc aaaatttaaa agtttgttta aaactctttt aataatatca
    44161 taatcaaagc ctttatagga aagatgttga gaaatttttt gatatatttt ctttttatct
    44221 tctttcttta ctctttcata cctttttttt gctagataca tagcattttc aaattcagtg
    44281 tccttgtcta attcagatac tgcttcattg attgtacttc tattgatacc tttattatat
    44341 aaattctgct taattctgtt tttgccacat ttatttaggt taacatttgt gttaacaatt
    44401 ttttgagcta atacactgtc atttacgaga ttataacttt taagaaaatc tatgaccctc
    44461 tcaattatat tttcatcaaa ttctgatgat agtttttctt ttaatttttt ttcagattgg
    44521 tcagcatgag ataaaatatt taatgcttta tttttagctt ttatatacat ttcatcatct
    44581 aaaataggtt ttaattcttc ttcatttatt tcattccctt tttttaaatt aaatgtataa
    44641 acaagttctt taaatatagc aataaaaaat ttatcatcta cataaatatt aactctatca
    44701 tcatttcttt tttgttgttc tatt
//
LOCUS       DAELXX010000019        44185 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.019, whole genome shotgun sequence.
ACCESSION   DAELXX010000019 DAELXX010000000
VERSION     DAELXX010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 44185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44185
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.019"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            50..1117
                     /locus_tag="KPH29_001014"
     CDS             50..1117
                     /locus_tag="KPH29_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HBG1466640.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESVILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEESKDISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            1136..2296
                     /locus_tag="KPH29_001015"
     CDS             1136..2296
                     /locus_tag="KPH29_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBG1466641.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPVSMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEETELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVSTILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            2309..4405
                     /locus_tag="KPH29_001016"
     CDS             2309..4405
                     /locus_tag="KPH29_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HBG1466642.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLIEKLNPFTNKIESLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPDFVDSTKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPMIHEREDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPVEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNSMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGFNKWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDNDLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTIITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTKEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(4564..4908)
                     /locus_tag="KPH29_001017"
     CDS             complement(4564..4908)
                     /locus_tag="KPH29_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBG1466643.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
     gene            5200..6090
                     /locus_tag="KPH29_001018"
     CDS             5200..6090
                     /locus_tag="KPH29_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBG1466644.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEVIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            6308..7594
                     /locus_tag="KPH29_001019"
     CDS             6308..7594
                     /locus_tag="KPH29_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBG1466645.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDICEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKKFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(7683..8126)
                     /locus_tag="KPH29_001020"
     CDS             complement(7683..8126)
                     /locus_tag="KPH29_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HBG1466646.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(8150..8944)
                     /locus_tag="KPH29_001021"
     CDS             complement(8150..8944)
                     /locus_tag="KPH29_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HBG1466647.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
     gene            complement(9510..9986)
                     /locus_tag="KPH29_001022"
     CDS             complement(9510..9986)
                     /locus_tag="KPH29_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG1466648.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPNVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSVPKWCSHWPKSVEKSGEFNWEDEIEF"
     gene            complement(10087..11256)
                     /locus_tag="KPH29_001023"
     CDS             complement(10087..11256)
                     /locus_tag="KPH29_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG1466649.1"
                     /translation="MLNIRTLCHEINDWIINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSINIGKMHAC
                     GHDAHTAILLGTCNILFKLKNYLNVNVKFFFQPAEETIGGAQLMIEDGCLENPNVKYI
                     FGLHVNPNINKNLIELKYNTLNASTDTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVMLHVKNVLSF"
     gene            11419..12282
                     /locus_tag="KPH29_001024"
     CDS             11419..12282
                     /locus_tag="KPH29_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBG1466650.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWDKFTYKSLVNALEFKDDLYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            12353..12946
                     /locus_tag="KPH29_001025"
     CDS             12353..12946
                     /locus_tag="KPH29_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HBG1466651.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(13197..13832)
                     /locus_tag="KPH29_001026"
     CDS             complement(13197..13832)
                     /locus_tag="KPH29_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBG1466652.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNRVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNIHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(13912..14643)
                     /locus_tag="KPH29_001027"
     CDS             complement(13912..14643)
                     /locus_tag="KPH29_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBG1466653.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLYMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQNNKECKIPTLEEVLILISPTD
                     LILNIELKTDKINYPNIEKDVLELILKYNMKNRVLISSFNSNSLKNFHKLDPSVKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEDDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
     gene            14974..17163
                     /locus_tag="KPH29_001028"
     CDS             14974..17163
                     /locus_tag="KPH29_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HBG1466654.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNNNQYEVPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKLGYELTEKITSSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEYNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDMYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKKALPE"
     gene            17574..19502
                     /locus_tag="KPH29_001029"
     CDS             17574..19502
                     /locus_tag="KPH29_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HBG1466655.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFFEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTTLPIVLSKVKNNEQE"
     gene            19489..19836
                     /locus_tag="KPH29_001030"
     CDS             19489..19836
                     /locus_tag="KPH29_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HBG1466656.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENSVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(20078..21997)
                     /locus_tag="KPH29_001031"
     CDS             complement(20078..21997)
                     /locus_tag="KPH29_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071223118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG1466657.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGLIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVKRGAQ
                     ARSTKQKARLQRFDELMNRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKRESVKKDDSTTLKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(22023..22829)
                     /locus_tag="KPH29_001032"
     CDS             complement(22023..22829)
                     /locus_tag="KPH29_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG1466658.1"
                     /translation="MNLILGDNMSKKSKRRKINKLKLTIVITVLFLALLSIYEILFSN
                     PNKKGNIKSQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGK
                     SMPTDEPISFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEK
                     IKLIDDYDAVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDV
                     TPKISTVYADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            complement(22888..24336)
                     /gene="ddlR"
                     /locus_tag="KPH29_001033"
     CDS             complement(22888..24336)
                     /gene="ddlR"
                     /locus_tag="KPH29_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HBG1466659.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDMEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            complement(24360..25259)
                     /locus_tag="KPH29_001034"
     CDS             complement(24360..25259)
                     /locus_tag="KPH29_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBG1466660.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            25517..26773
                     /locus_tag="KPH29_001035"
     CDS             25517..26773
                     /locus_tag="KPH29_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBG1466661.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGVAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            26843..27622
                     /locus_tag="KPH29_001036"
     CDS             26843..27622
                     /locus_tag="KPH29_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG1466662.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(27726..28310)
                     /locus_tag="KPH29_001037"
     CDS             complement(27726..28310)
                     /locus_tag="KPH29_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HBG1466663.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYHIANCG"
     gene            complement(28510..29940)
                     /locus_tag="KPH29_001038"
     CDS             complement(28510..29940)
                     /locus_tag="KPH29_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG1466664.1"
                     /translation="MNLDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQTGKKLIITATPIFDENGNLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNADLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSDFNLNKTISKEAMDAIANYRFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKVIISDSKSTNYKNKNFDTLIKEYEKNIILDAYEKFG
                     SSYKVAKHLEISQSKANRLIRKYTNT"
     gene            30256..31200
                     /locus_tag="KPH29_001039"
     CDS             30256..31200
                     /locus_tag="KPH29_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBG1466665.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKISKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKVCYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
     gene            complement(31471..32406)
                     /locus_tag="KPH29_001040"
     CDS             complement(31471..32406)
                     /locus_tag="KPH29_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBG1466666.1"
                     /translation="MDKFKYAFDDKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNQIDDTLKARNSYQGCKFV"
     gene            complement(32526..33119)
                     /locus_tag="KPH29_001041"
     CDS             complement(32526..33119)
                     /locus_tag="KPH29_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBG1466667.1"
                     /translation="MNFCYNCSIDFGGDFMYTNFNIDFNNFNKKENATKFMLMGVLLI
                     ILGLLCLTFKTLGIKLISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVL
                     IIACILLFVFPQKIQSIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGIC
                     LILSPLFLSRFIVSIMSFFIILFGLVLFFSGNTARKY"
     gene            33226..33654
                     /locus_tag="KPH29_001042"
     CDS             33226..33654
                     /locus_tag="KPH29_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBG1466668.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            33721..34389
                     /locus_tag="KPH29_001043"
     CDS             33721..34389
                     /locus_tag="KPH29_001043"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HBG1466669.1"
                     /translation="MKEVLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTIS
                     EEHGKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAY
                     GDIDIESHRKWDTDRCEAVGMKVDLPLWQESREDLVYEFIDSGFCSVVTKVNLKHLGE
                     EFLGKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGY
                     LEIK"
     gene            complement(34507..35862)
                     /locus_tag="KPH29_001044"
     CDS             complement(34507..35862)
                     /locus_tag="KPH29_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG1466670.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATVLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFAICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(36404..36897)
                     /locus_tag="KPH29_001045"
                     /pseudo
     CDS             complement(36404..36897)
                     /locus_tag="KPH29_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429361.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            36993..37349
                     /locus_tag="KPH29_001046"
     CDS             36993..37349
                     /locus_tag="KPH29_001046"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBG1466671.1"
                     /translation="MVFETFDKVIKLHPNAKPVFHSDRGFQYTSKVFESKLLAQGMIQ
                     SMSRAGRCIDNAPMEGIWGILKVEMYYLRKFDTYEQLVKIIETYIYYYNNFRYQKRLN
                     SMSPLEFRKYLSTEIA"
     repeat_region   37880..38566
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=37946..37974
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
     gene            complement(38958..40691)
                     /locus_tag="KPH29_001047"
     CDS             complement(38958..40691)
                     /locus_tag="KPH29_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG1466672.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDINEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDVFETVSEDENRCMVLGRYTVRTKENAQMIL
                     EVNQRCTFEIIEDRGKLLVKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDSTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
     gene            complement(40931..41776)
                     /locus_tag="KPH29_001048"
     CDS             complement(40931..41776)
                     /locus_tag="KPH29_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG1466673.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQLEALRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMYKNKEKNKKKF
                     DKNDY"
     gene            complement(41807..44041)
                     /locus_tag="KPH29_001049"
     CDS             complement(41807..44041)
                     /locus_tag="KPH29_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG1466674.1"
                     /translation="MQNHNYIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNRE
                     FMYQYIDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELNNMGATIIESSRELKIRLPNEKEIYSNVTFSIGSTI
                     WNGEPNMTTLILFNQVYSALKKAKSEGKNRYILFDFKDNSFYNKLENDKEFLSEISHV
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     HLFEENCKNIRRWIDNNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIE
                     ITENILIENTNTIIQQLAELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKEFEKLLSKENNSEKMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEASEHQ
                     KYSNFYFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNILSQ
                     FYSMLPVGIIRYDLFGDMIITYMNEEMFDIIGYTKEQFFGDMGGNLRAIVHPDDLDFV
                     YKKSLDMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYVDVNKIKN
                     LTKI"
BASE COUNT        16017 a         5532 c         6597 g        16039 t
ORIGIN      
        1 ataaaatttt aaaaattgaa ttataacttc aaaaaataag gagggtaata tgaagataac
       61 agatatcaaa tttgaaaagc ttagaattaa attaaaaaaa cctgtagtag tttcatttgg
      121 agtaattgaa tatggagaaa gtgtaatttt aaaaattgaa actgatgaag ggtattgtgg
      181 atttggcgaa gcagcacctc ttgcagcagt tacaggtgaa gttttggata atgttctttc
      241 agtattgctt atgtttaaaa aagagttaat aggcaaagac ccactagata ttgaaaccat
      301 acatacaatc atggatggaa ttattatagg taatacttct gcaaaagcag ctattgacat
      361 agctttatat gatattaaag gaaaaattat gaatgcacct ctttataaag ttcttggagg
      421 atttgatagt aaagtccaaa cggatattac tataagtata gataagccag aaaaaatggc
      481 agtagaagct ttagaaagag taaaagaagg ttttaggata ttgaagctta aagcaggtat
      541 aaatcctgaa gacgatattg aagctgtaaa attaattaga gaagccgttg gagagagtat
      601 aagaattaga attgatgcaa atcaaggatg gaacgtaaat agcagtataa atactatcaa
      661 aaaattagaa gaatttaatg ttgatgcaat tgaacaagca cttccacatt gggatttaga
      721 tggtactgct tacataagaa ataaaagtaa tacaaaaatt atgatagatg aatcgttaca
      781 ttcaccaata gatgcaatta aggctataaa aaagaatgca gttgatacat ttaatattaa
      841 gctgatgaaa tcaagtggaa tatatcctgc tataaaaatc aacaacatag ctgaggcaag
      901 tggagtaaat tgtatgttag gatgtatgct ggaaacgaga attggtataa cagcagcagc
      961 aaatttaata gcatctaaaa agaacataac agaagctgac cttgatagtt ttatgttttg
     1021 tgaagaatca aaagatatat ctggaggatt tgtaatggat ggagatatta tgaatcttgt
     1081 caataaacca ggtttaggaa tagaagtaaa cttatgataa atttggaggt taaatatgaa
     1141 tacacttttt tcaaatccag gtagtatact tgctgtaatg acatcaatga ttgcattggg
     1201 tttttactta caaagataca aagccattaa aagcttagga ccagcactta caataatcat
     1261 aatgggaata atactttcaa acttaaaagt agttccagta agtactgagt tatatggaac
     1321 tatttcaaca tatgctatcc cagtttctat gactataatg ttgatgagtg ttgacttaaa
     1381 agaaatgaca aaattatcaa gagaaccttt aattgcaatt tttgtagctg tattaactgt
     1441 aagtatcatg gcatttttat ttggattagt atttgcagaa aaaatatcag aaggttggaa
     1501 ggttgctgga atgtttgtag gtacatacac tggtggaagt gctaatctta cagctattgg
     1561 aacaggcctt aatgtaagta gacaaactct tgctgcagca aatgcagcag actatgtaat
     1621 tggagtacca acattgatat ttatgtttgc tctaccagca atattaaaaa attcaaagaa
     1681 gtttaaaaaa ctttggccat atcatgtaga agaaacagaa ttagaagatt gtcaaaatga
     1741 agaatttatg gagtcaaaag aatggagtat taaagatata gcttggatgt tggctatagg
     1801 ttttgttgtt acagaagttt caacaatact tgctggatat ttcaattcaa gttttagtag
     1861 tgcagcaaga atacttttag ttacaacaat ttcaattata attgctcaat taaagccagt
     1921 aaagaaacta aagggaaatt tagatttagg attatttgtt gcattattct ttttatgtac
     1981 aataggtttt tcagtagata taaaagagtt tttaggttca acttttacta taacacttta
     2041 ctgttttagc ataatttttg cttcatttgt ttttcattta ggcataacta gacttttaaa
     2101 gataaagtat caatatgtaa tactttctat agtaggagca attgctgatg gtcctacttc
     2161 agctttagta gctgcttctg caaagtggaa ttcgcttgta agtgtagcag tcgtaatggg
     2221 agtaattggt ggagtattag gaaattatgc aggtatatca gtggcatatg caataaagat
     2281 gtctctagga ttataggggg gagtttaatt gataaaattt aaattataca tatctggact
     2341 atacagtgga aatgtaatct ttgatggcga tttattaatt gaaaagttga atccttttac
     2401 aaataaaata gaaagtttaa aaccaataag caaagaagaa aatacatact atttaaatct
     2461 tacaaaaata gatttaaagt ctttatttaa taactttgat gtatatacta aaagcttagt
     2521 aaacacagat aaagacacag ttataaataa cttaggagaa acgcattcta aacttaatga
     2581 atatatatgg gttcaaagaa acaaaaagtt cccactagat ataattattg tagataataa
     2641 aatagtaggt tttatctgtt tatccagaga aacttgtact atattaatta tggatggata
     2701 tgaagagtat actgtattaa aagaatggga aaaaacccat aaaaatgaag agatatattc
     2761 tataagattt ggtggaaatt atatgattga tatgaaagat ggtataaaac tttctacaga
     2821 tgtatatctc cctgattttg tagattctac caaaaaagca ccaacaattc ttatgagaac
     2881 tccatatgga aaagaaaatg ataaagaaat atattataaa tatgtacaaa gaggatatgc
     2941 tgttgtaata caagatgtta ggggaagaaa tgaatcagaa ggtaagtggg aacctatgat
     3001 tcatgagagg gaagatggag atagtaccat aaattggata gtatcacagg aatggtcaag
     3061 tggaatagtt ggaatgcttg gagcatcata tcttggttat gtccaatggg cagctgcatc
     3121 ttcaggcaat aagcatctta aagctttagt tagtatagta acatcaggaa gcccttttat
     3181 tgatatacca agaaaaggag gagcttttgt atctggtatg cttgcatggg catttatggt
     3241 atcaagaaat aaagttgata gaagtaaaat ggtaagagat gattgggatg atgttttaaa
     3301 catacgacca atagaaaata tacctgttga agctcttgga tatagaattg aatttctgga
     3361 agaatggtta aagagagtag aaaaagatga gtattgggat ttgatggatt ggcatttaca
     3421 aaaagataaa ataaatgtac ctgcattggt tgtatctggg tggtatgatg ataatagtat
     3481 gggaactaca gaggccttag atgttataaa ggactatgaa aaaggaaaga gaaaggctat
     3541 tctaggtcca tggatgcata attcaaatac tttaagagat ataaatggta tttctttagg
     3601 aaatagttca ttaagatatg atttggatta taattatcta ttatggtttg ataaatactt
     3661 aaaaggtata gaaaataaca tagatacgac tgctccagta gagtattact ctgtaggttt
     3721 taataagtgg aagacagaag aaaattggcc aataataaat aagatagata aaagtatgta
     3781 tctaataagt gatggaaatg ctaacacatc tttaggaaat ggtagattag tatttgataa
     3841 tgatttagaa gaaaagtatg atagttatat atataatcca aaagacccat cagtacaact
     3901 aattgatatg tcagagaatg aagttggagt tccaaataac tacaaagatt tagaaaaaag
     3961 aagtgatatg ttgtgttata catcagatgc ttttagtgaa gaatttactg taacaggtga
     4021 tataaagtta gagttttttg catcaagttc tgccaaagat actgactggg ttataaaaat
     4081 tatggacgtg gatttagatg gaaattcaat taaattagct gatggtatac ttagtgctag
     4141 atttagaaat agtttttata agtcagagtt tatggaagaa ggagagattt ataagtttac
     4201 gattataact tcaaaaatat caaacacgtt taaagttggt cataaaatta gactagatat
     4261 aacgtctagt gctaaaaact ttattttcca aaatagcaac acgaaagagg gatataatag
     4321 tattgaatat atagaagcaa agaatacgat ttaccatgga ggtaaatatc catctaaatt
     4381 gatattgcca atagaaaata agtaaattta tatagattaa atagtgagga ggtgtctcaa
     4441 aatgaatttt ttagttcgtg agacactttc ttttatacga aatcaaatat attttcatca
     4501 tttcaacaat gaaaaagagg cttatctctc tgttttgaga tagcctccta taagctaaat
     4561 tgattatttt gtagctgcaa caacgctcat ttcaactaac aaatgctctc tagcaagtct
     4621 agcttcaaca caagctctag caggttcaaa tcctttttca acccaagcat cccaaacaga
     4681 gttcatagct tcaaagtctt tcatatctct taaataaata gtaacagata gcaaatgttg
     4741 cttatctgaa ccatatttgt ttaataaatc ttctatttta gctaaaactt cagttgtttg
     4801 ttcaattact cctccttcag catgagtttg tccacataaa taaactgtat tgttatgaac
     4861 tacagctcta ctcattcttc cagttccttc atatctcttt atttccatat tttaaatacc
     4921 tccatagtta agaaatctat taaaaatcta aataataaat taaaaaaagt ctatactata
     4981 taaaattata ttatatattc tgataataat ccataaaaac atataacttt ttagctataa
     5041 ttattattgt tattaattga aaagacttat tagttacttg acatagttta cacttttaat
     5101 tatagtataa attggaatat ttagataatt attatttaat gtataagcta agtttttaat
     5161 taatatggct ttatacattt atatagaaaa gaggtacgta tgagtagcac aatcaaatca
     5221 aatcgaataa aaggtataat gtttataata gcatcagcgc ttggctttgc aatgatgtct
     5281 gcattcgtaa agctagctgg agatttacca agttttcaaa aagtgttctt tagaaatctg
     5341 gtatcagcta taatagcttt ttggcttatc ataaaacata aaggaagtct aactggtaaa
     5401 aaagaaaatt taaaagtact tttatatagg tcaatatttg gaactctagg agttatattt
     5461 aactactatg caattgacaa gttgatgctt tcagatgcta atatgctaaa taaaatgagt
     5521 cccttccttg tagtaatatt ttgtgcaatt gctctaaaag aaaaaataaa tgtaaagcag
     5581 ataggagcta ttataattgc atttatagga gctttattca taataaaacc aacatttagt
     5641 gtagaagtaa ttccatatct tgggggaatt gcaggagctg tatttgcagc aatggcatat
     5701 acttgtttaa gagttcttgg agatagagaa gattactata caattgtatt tttcttttca
     5761 gttttttcat tagttactgt agggccaatt gcttttgcag tatatgaacc aatgaccctt
     5821 atgcaactta tatatctatt actaggtgga gtatttgcaa gtctaggtca atttggaata
     5881 actttagcat ataaatatgc acctgctaag gaaatctcta tatttgacta tagtaatata
     5941 atcttttcag caattttaag tatattctta ttcaatgtct atccagatat gttaagtgtt
     6001 gttggatatt taatagtatt ttcggcagca ttttatatgt ttttatacaa taagaagtta
     6061 gataaattag acaaagaaaa gaagaagtaa actagataaa agtgttttta taatttgtat
     6121 tgaatattta agtctttgta ttaaaattaa agaaaattta attaaaaatg tttggtatat
     6181 taacttataa cagcagttaa aattgaaaaa ttataactgc tgtttctttt attaaaaata
     6241 tccaaatgtc tgtaaaaata gactataata gagtagtata aataatataa aggggataaa
     6301 ttaaagtatg gaaaaagtga aagttgctgt gattggagca ggtaatagag gaacgtatgc
     6361 atatgctcca tatatttatg aaaattctga tatatgtgaa atagttgctg tagcagaacc
     6421 taaaaaagga agacgagaac tatttacaca aaaatataac ctagatagta aaaatgtatt
     6481 tgaaacttta gaagactttt ttaaacatga caagatggca gatgcagtaa taatagctac
     6541 aaatgatgat agacattatg atgtagcaaa actagctctt gaaaaaggtt atcatgtact
     6601 attagaaaag ccaatgtcta atagtttaga tggtttggta catatagatg acttgtgtga
     6661 taagtataaa gataagattt ttatgatttg ccatgtactg agatattctc cattttataa
     6721 taaattaaaa gaaattgtag aaagcaaaaa attaggagag ctagttagta tacaatataa
     6781 tgaaaatata ggatattggc attttgcaca tagctttact agagggaatt ggagaaatag
     6841 caatgaaacc agccctttaa ttttagcaaa gagttgtcat gacatggata ttttgttata
     6901 tttagtagga agtagatgca aaaaaatatc tgcatttgga agcttaaagc atcttaataa
     6961 ccaaaatgct agtggagaga tggcacaaaa ttgtcttcag tgtttagttg aaaaaaaatg
     7021 tccatattca gcaaagagaa tttatttaga aaaagataga agtataaata gagctgttca
     7081 cataaatcca actgaagaga atctattaaa tatacttaag acaagtccat atggaagatg
     7141 tgtttacaga tgtgataata atgttgtaga caatatggta aatatattac agtttgaaaa
     7201 tggagtgacg gctactttta atttatgtgc atttactaaa gaaaatggaa gaactataaa
     7261 gttaatgttt agtcatggtg aagttggtgg ggatttaaat aaaaatgaga taagaattaa
     7321 aaaatttgga aaaaacgaag aaatagttat gaatccatca aatcaacaga atatggtaga
     7381 ggaatatgat agaaatctta tagctgaatt tattaaatta gtttcaaata aagaactaga
     7441 aaagggtaga gtagcagcaa aagaagctat acaaagtcat gtaatggcat ttgcagcaga
     7501 atattctagg gtcagtgatg aagtagtgta tatagaagaa ttttttgata gtgctaagca
     7561 aatgactaag gagatagaag aaactatatt ttaattaata aaaatttatt aaataaaata
     7621 aagatagatt aaataataga aatacgaaaa tatgtgttct attaaggaat ctatcttttt
     7681 tattaaggtt ttttcttatc cctatcaaaa aaatccatac attctgataa aagataagaa
     7741 agtaaagcga ataagatttc tgtacttaaa ggaatcgtta ctgaatccat aaaggtatca
     7801 attatgttca tagctagaaa agttagtata aaatcaatta taaattcgca aagtttatat
     7861 attgagtatg gtaatttgtt tacatatctg aatatatcta aaatagtagt gcatagaaaa
     7921 tcaattggag tggttataca aaaatatatt aaaaagaaaa ttaatacagc tccaagagat
     7981 ttatattcaa atcctaagaa tgataagaag ccaattccta taaccgtgaa aatacaaaaa
     8041 gttataccaa aaaaaacagc aaaaaataaa aatacagaaa taaattcgcc taaaggaatc
     8101 cttcttttat tacttatctt aatcaactcc ttaatttatg atatgaaatt tatataaata
     8161 ataacattct taaaaattca taatagtcct ctgtttcaaa tagtactgtt tgagaatcta
     8221 tttttttcat atttggataa aaagaggcct taaaagcttt ataatgttct ctaaatttta
     8281 tttctatctt aaatttatct ggcaatttta ttaagtgcct agataaatct ccagataaag
     8341 cttttttgac acctttttca atttttttta gtgcaaggtt tggatggatt gaaattgaac
     8401 catttccaat tccttttgac acaggaacag tgactatatt tggatttagt ttttttgcac
     8461 tctcacaaag catttcatct ccacttaaaa atgctacagg aacattataa tatgcagcaa
     8521 tataagcatt tatagtaaat tcagatgcta tttcatcatt tattttaaaa tagtcataat
     8581 caatatccat agtatgagaa agtggattac cattacaagt agctccagag tgatagccgg
     8641 tgaatataca tgcatcaaag ctttcatcaa ttccagccat cataactagt gggtctttgg
     8701 tccatcctct aagtattttg acgttttcag gtaaaatatt tggattaata tttctaccac
     8761 tgtcatgtgc atctttaaca agtatttcat gtacaccaga ttgagtagct ccatcacatg
     8821 ctgctgttat ctctttactc atttgtgttc tatagtgttg tgaaaattca cattcaagtt
     8881 ttgtttcatc ccaatttaca ataccacaag taccttctat atcagcactt aaatacaatt
     8941 tcataatata taccccctta tgtttattat tgatgataat aacagataac agaaaataca
     9001 taatcaataa agggcataaa aatattatgt cttactatta attattatta aatctgtaac
     9061 tgaaaaataa ctaaattggt attaaaaaat ataacactat gtgtcattta tttatatagt
     9121 tttttatatc tgatgtttat ataaaattgt aacatataag tggttacatt tactagttta
     9181 acatagttaa aatagttagg tattattttt aagttaaatt gattattata aaatcgtaga
     9241 ataaaagaaa aatttaattt attaaaatgc atgaaatgtt ttttcctcaa cataatgcta
     9301 aattaaactc aaaaagaaat aatagaaaaa tcttatgtag cttagaaaga atgtgaatga
     9361 tttaagggaa aaatagaaat ttattatata atattaggct gtattatgta atacagccta
     9421 atattatatg tatatgtgac ataaaatctt ggtaagtgta aataaaataa atgtggaaaa
     9481 caaattaatg aaatcaagaa aaaagtaatt taaaattcaa tttcatcttc ccaattaaat
     9541 tctcctgatt tttctacact cttaggccag tgactacacc atttaggaac ggacggattt
     9601 tcaaccctat caacgcttgg agtatatggc tttaagtcta taacaggact attatcatta
     9661 gcatcaatat atggtatttt tattatacca ttttcatggt cgatattgat aatttgtaca
     9721 gcagtcaatg ctattggatt gggtcttata ggtgaccttg ttgcaaatat tcctataacg
     9781 tttggtccac ttttataagg ttttggggtt tctaaaatac ttcttagttc aggattatca
     9841 aacccatcag cccaccaaaa tacatttaaa tgactgaaat cgtcgagttc atttaatgca
     9901 ggtacatact ttttgtctaa tttaataaag acctcttcgc cttgaataca tattttacct
     9961 ataggattta ttaatatttc tttcataata ttacctccaa ctatttaatt tatcatttgt
    10021 aattttaggg tataacatga catcatgtga gggtcaatat aaaataataa ataaaaatat
    10081 taatagttag aatgataata cattttttac atggagcatg acaccagtaa taaggcaatc
    10141 ttcgtcaata tcaaaagtag aggtatgcaa aggggaagta atgttttttt cacgatttgc
    10201 acaaccaagg tggtaaaaag ctccttcaca gtgtcttaga aaatacgaaa agtcttcagc
    10261 cccaagagtg ggcgagtctt taaggataat attttcttct ccaaacaatt ctacagcatt
    10321 ttgcttgacg taatctacta gatggtttga attgataaga gctggataac cttcctcaat
    10381 ttcaacagaa atacttcctc caaatgtttt acaagtaaaa tcgcaaattt ctctaattcg
    10441 tttttttgaa aactctctag tttcaggagt caaggttctc aaagtacccc ttataaccac
    10501 tttgtcacaa actatatttt ccttgattcc accttcaatc tttcctagag aaataaccac
    10561 agaatcagtt ggatttgtgt ttcgactaac gattgtctgt agagctgtaa taatatgagc
    10621 agatataaca atagcatcaa taccttgatg tggataagct ccatgacatt ttgaaccatg
    10681 tacagttaat tgaagtgtat cggtggaagc atttagagtg ttgtatttta attcaattag
    10741 atttttgttg atattgggat ttacatggag tccaaatata tattttacat ttgggttttc
    10801 aagacatcca tcttctatca taagttgagc cccaccaata gtttcctcgg caggttgaaa
    10861 aaagaatttt acatttacat ttaagtaatt ctttaattta aatagtatat tacaagtacc
    10921 cagtaaaata gcagtgtgtg catcatgacc acatgcatgc attttaccaa tattaataga
    10981 tttataagaa taattaagtt cttctgttat aggtaaggca tctatatcag ctcttatagc
    11041 aactgtttca aagtttggac tcacgttaat gtatgcagtt atccctgtat gattcttata
    11101 ttcaatataa tttataccta tttcatttaa gtatttaata atttttttct tagtttgaaa
    11161 ttcttctaat ccaagttctg gagtcttatg taaatctctt cttatattta taatccaatc
    11221 atttatctca tgacaaagag ttcttatatt aagcataaaa cacatcctaa ctatattaaa
    11281 aatttatttt tcttcattaa ttatattatt ttatgtaaat cattattatg tatcaagtac
    11341 aaataaaata tcttataatt atattagaga tatttagtat ggagaagaat atatcatttt
    11401 ataagaagag gtgtttaaat gttaggaata attgcgccat ctgggccatt aagaaataca
    11461 agtttggagg aaataaagtt taatttagaa agttatggtt acgaagttaa gttttctgaa
    11521 agttgtagtt taaattataa aggttattta gctggtaatg atgatattag agcaagggat
    11581 atagaagata tgtttttaga taaagatgta gatataatta tgtgcttaag aggaggttat
    11641 ggaacaacaa gaatattgga taaaataaat tatgatataa taaagcaaaa tccaaaacca
    11701 tttataggtt tttctgatat aacaggttta aatttagctt tttataaaaa ttgtggactt
    11761 ttaccatatc atggaataat ggcagcaaat gtaggtaaat gggataaatt tacctataaa
    11821 tcattggtaa atgctttaga atttaaagat gacttatatt tagaaaatcc aaaagaagaa
    11881 aagatttata ctgtatgtga aggtaaagca gagggaataa taatgggagg aaatttatcc
    11941 ttaataattg ccacaatggg aacaaaatat gaaatagatg caaaagataa aattttgttt
    12001 attgaggaaa ttggagaacc tcaatataaa ctggatagaa tgttaacaca actatattct
    12061 tcaggaaaac ttgaggaatg taatggtata atttttggag attttaagga ttgtattgaa
    12121 gaaaatgatt tgatggaatt actaatagaa tttgcaaata aggtcaataa accttctata
    12181 tataatcttc agtcaggaca ttgtatccca atgataacta ttcctttggg cagaatgtgc
    12241 gaattagatg ctacagaaaa aatcgttaaa ttaaaaaaat agtgtgtata taaatataat
    12301 agaaatatgg tataatctta tacaagtaaa aatttagcta ggaggatgaa aaatgtctga
    12361 tgatagaata atagacttta acgaactaaa gaacaaagtt aaagattctg atgtagataa
    12421 atttgagcaa tacatatata acttatattt ttctgttatg gatggaaaga tgtccatggc
    12481 tgagttttca agaaaaatat tcgattatat gagagataac aacatatctc aagaaaaatt
    12541 tatgaaaata caaaagcaat ttatggagag atatggtatg gacactgaag aagtagaaaa
    12601 acaacttaga aactttggaa tcgacccttc tacagcaggt tttatgagta ataatacttc
    12661 cagcaaagta tctacagaag atttagagtc atttaagaag agtgcaggat tttatgagaa
    12721 atatggagaa aaaatccaac caaagagttg tataacaaca tttataaaaa atgacttaaa
    12781 tgatattaat gtaataatcg accaagaaaa gataatgctt tgtagtgata gaaaaataaa
    12841 tcttatggat tcagaactaa atgagttttt attagaatat aagaatatgt ttaataaaaa
    12901 aataaaggta gtaatgtgcg aaactacgaa taagtatgat tattaaaaaa tgaatttaaa
    12961 tactgaagta gtatcaatta attgtactac ttaaaaataa aaatcgcaat ttattattcc
    13021 ttataaattt gtataaggaa tatctaaact gcgatttttt atattgtgaa gattagtcat
    13081 aatagatagt gttttaaatt gtaatcttct cactaaaact gtgtttttct attagaaatt
    13141 ttattaaaaa taaatatctg ataattgtat tatatgggat gtaaagtatt agaggcttaa
    13201 cgaatctctt ttaatacatt aactccattt tgaatcatac tgttgaaaaa tactaagttt
    13261 aaaatctcag cagggtgaat gttaggaata tcataagtat ctactaaatt cataggtaca
    13321 actatatttt tttcaatatt atgttgatta aaatatgatt ttagagttat tgcaaattga
    13381 tatatgcata tatctgtgca atcgccaact ataattatat tatctatatt atcaaaatcc
    13441 aaattttcta atgcaaaaaa tccattagta gaatttttag gaagaatttg taggttttct
    13501 atcgacttaa gttcatcaac caattcactt tctacatcat tttccaaaca gtgaacagga
    13561 taactcaaaa gttctattga tttaggagta tgagaatcag taaatgctat tactctattt
    13621 aatttgtttg atattttttt tgtaaacatt tcaattggtt ttattaaaga ctctactcta
    13681 ggagagtata atgcaccttg tcttgcaaag ccattattaa catcaattat aaataaagca
    13741 gttttactca aatcataatt ttctattttt tctattggta gattatctaa attactcttt
    13801 aaagcttcaa gttcatttaa taagttatcc atagttatac ctccaagata aatttaataa
    13861 aattgtacat attaattata taaaagtcaa ggagtacaaa ctattgtata attagctgta
    13921 ttgttttgag tgtaaaagat ttgaagcaat atctggatag ttagtaaaaa taccatctac
    13981 attgagtttt agacagtgta gtatatcatc ctcttcattt acagtatata cattgacacc
    14041 aagtagattt ttgtgacaaa gttcaattag agattcattt actaaaacta gaggtggatg
    14101 aaggtaacta ttacccagaa atttagcaaa attgactaca ttgtttatag gtaaataaca
    14161 caataatcct gtttttacac tagggtctaa tttatgaaag ttttttaagc tattgctgtt
    14221 gaaactagat ataagaactc tatttttcat attatacttt aaaataagct ctaaaacgtc
    14281 tttttctata ttgggatagt ttattttgtc agtttttagt tctatattta aaataaggtc
    14341 tgtaggtgaa attagaatta atacttcttc taaagttgga attttacatt ctttattgtt
    14401 ttgatatcct tcaaaagatg agtttagagt ttttaatttt cttaaagtaa agtcttttac
    14461 aaaaccatgt ccattaaaag ttctatcaac cttttcatca tgaataacta caatttttcc
    14521 atccttagtt ctgtggacat caagttctat tccatatata tccatataca aacatttttt
    14581 aaaagctaga atagtatttt ctggatactt tccactaaac cccctatgag cataaatatt
    14641 catttatatg gttccctcct ataagtataa cttatcttat aaaaatttaa ttaatataat
    14701 atatcatatt ccaaaaggta ttgttatata ttagttaaat aactatttgt aatttatata
    14761 ttatataaat gtagaaaaat aaaatcatat atgtgttata atatatttta aacattgtaa
    14821 atattacgaa tttagtagtc ataataagtg taattttatg caagctcatg tttttagagc
    14881 ttgcatatgt attattaata tatttatata ttgtgaaaat aaaattccaa aagaattttt
    14941 tctatagact tagtagtaag ggggaatttt gatatgtatg aagttaattt agaacgagct
    15001 atgaattatt ggatggattt gttagatgat tatacgaata tagcaaaaat tccaaatgat
    15061 tttttttata aagattgttc tgatgaagta gtagatgagg tttttaagat gaatattgag
    15121 aaacatgaaa ggttaattca atattctaaa gtgaacaata ttagcataga ctctatcata
    15181 gaaactatat atggccttat acttcaaaag tatatatatg aagatgatgt agtttttggt
    15241 aaaacaatga atataattcc aataagagta agagttagac aaagtgaaaa atttatagat
    15301 gttgtaagga agctaaaaag gcagtggagc aggtctttag agtatggata ctatttgtta
    15361 tcagatatag agaataaaaa tcatctaaat actaagctta taaacacaat atttatagct
    15421 aaaagttttg atttatcata tgattatgac atagaaaaaa aaggatatga tttatctgta
    15481 atagtatata ctaagagggc acttaaaata aaaataaagt ataaaccatc tatatataaa
    15541 agagaaacta ttagaagaat tttaaatcaa tttgattata taatagagca aataatagaa
    15601 gataatgata ttttaataga agatttagaa tttgtatgta aggaagaaga aaaagtatta
    15661 ttggaagact ttaataataa tcaatatgaa gtaccctatg ataaaagtta tgttgattta
    15721 tttagggagc aagttaaggt aacacctagt aatatagctg ctagggatga aaatagaaag
    15781 ttaacatata aagaattgga tgaggtaact gataaacttg caggttattt aaatcatata
    15841 ggagtggaaa gtgaagacat agtagctgta atgttaccaa gagatattaa cataattatt
    15901 acagcaatag ggattatgaa atcaggggga gcattttttc caatagacac gagtaatcca
    15961 gaagaaagat tgaattacct tcttgaagat agtaatgcaa aagtagtgat aactacagat
    16021 gaactcaaaa gtaaggttgt aaatgagaat accatagtac tcgatataaa tgatgaagaa
    16081 atgtttaagc ttggatatga attaactgaa aaaataacat catcaaattg tgcatatacg
    16141 atatctactt cagggtcaac tggtaggcca aagaccatag ctatagaaca taagagttta
    16201 gtaaatatgt gttattatag cgtaaaatct ataagcgcta cagaaaatga tatatgtggt
    16261 atatacttaa gtttcagttt tgatgctgta atgaaacaac tattccctta tttgcttgta
    16321 ggtgcatcaa tagatattat gcctgaagaa gctaaatttg atgaatatac agtaaatgaa
    16381 tactgtgagg aaaatgacat aactatacta gcattgccaa cagcatttgc aaggttattt
    16441 atacaaaatt gcaataataa ttcacttaga gttgtacaga ctggaggaga aagattaaaa
    16501 ggatatagaa agcgtaatta tgaactatat aatgagtatg gaccaaccga atttactgtt
    16561 gtatcgactt cttttcatgt ggacagagaa tatggaaaaa taccaatagg aaaacctata
    16621 tttaatactt atgcatatgt attagataaa aagaacaaac tatgtccaat tggagcacca
    16681 ggagagctat gtctatcagg cattcaaata tcaagaggat atttaaataa aaaagggcta
    16741 acagagcaag tatttgtaga gaatccttac aaaacttgtg aatataataa gttaatgtat
    16801 agaactggtg atttagtaag atggttagat gatggaaatt tagattatat tggaaggatg
    16861 gacaatcaag taaaaattga tgagtttagg atagagctat atgagattga aaatataata
    16921 aataatatta cagaaattaa aagtgttgtt tgtatatcga gaacaaatga tgacggggat
    16981 atgtatatat gtgcatatta tgtaattgat gaggaagata gtggtaagat aaatgaaaga
    17041 accattagag aatatttaaa tgagcatcta cctccatata tgattccaac aataataatg
    17101 aggatagata aaataccagt tacaccaata ggaaaagtaa ataaaaaagc actaccagag
    17161 tgatttttta ttatgacagg tgttgtaaag tgattccata catttcttta tgacacctat
    17221 ttttctgagg aaatcaattg atgtaaaaaa aagaattttc tgtatatatt tactatggtg
    17281 aaaatttatt tataagaatg attattgtat tttttatact tttccgtgtt ttaaagcatt
    17341 ttttttacaa aattaatttt acattaaaaa ttttttttat tttatatctt aatagttttt
    17401 agtagaattg aaaaataaaa agaaaattag cataaaaata tctaaaagta gaaattaata
    17461 atatagggtt aaattttagt agttttgact cgctatttgt aaaataataa aaagttaagt
    17521 tttatatgta tatatgttat aatatataca aattgaaaag gggtgaaaat tttatgaaca
    17581 aaaaattacc taagggtgct tatggtgagg ttagtggaaa agattacgtt ccatatatca
    17641 cagataagtc cagaaccggt ggaaatgtag ctgtactgat tatcggtatt attttagctg
    17701 ccatttttgc cgcatctaca acttactctg gaatgaaagc cggtcttaca gttgctgcag
    17761 gtattccagg agctataata ggttctgctt ttgttggagc atttgctcgt tcaaagggta
    17821 ttcttggtaa aaacttaatc caagggatgt caagtggtgg agaatctgtt gcaagtggat
    17881 ttattttcgt attaccagca gttatattaa ttggaagcca aattacattc tttgaaggcc
    17941 tagctgttgg agttggtgga gttttatttg gtataggaat tgctgctata gttcacaact
    18001 atctaatagt tgaagaacat ggtaaactta tgtatccaga atcaatggct atatctgaga
    18061 cacttgttgc ttcagaggct ggtggagatt caattaagta catgggaatt ggatttgtaa
    18121 taagtggatt tattactgta ttaactggtt cattcttaaa tgtagcaaac aatgtaatga
    18181 gtcttgtagg aagcaaattc tacaaatgga aatttgatat tgaagtaaat cctctacttt
    18241 taggaattgg atttatagta gggcttgaag tttctttaac aatgtttgca ggaagtattt
    18301 tatcaaactt tggtatagca cctttaatag gatactttac agatatggct aaagatggag
    18361 ctatggtatg gaataatcct gcaatgcctc ttaaccaaat ggatgtcgga gcaatatcta
    18421 gtagttatgt aaaatatata ggtgctggta tgatgctttg tggaggtatt attggtgcaa
    18481 taaaacttat accaactata atcgcttcta taaaagaaac acttaaagct aaatcaagtg
    18541 ctggagaagg agaagaaggt tcatctatac aaatgatact tttattaggt ggagtagtta
    18601 ttggattttt agcagcattc ttaatttctg gtaacattgt tatggcaatt attggagcta
    18661 tcatatctct tttattatct ttattatttg tcatagtagc tggtcgttta acaggaacaa
    18721 ttggaacatc aaatcttcca gtttctggta tgactatagc ttctctagtt attgtaacat
    18781 tagtgtttgt tataatggga tggactgatt tagaagctaa taaatcatta cttttatttg
    18841 gttcatttat agttgttgct attgctatag ctggaggtta tactcaatct caaaaagtaa
    18901 cttatattat tggtggtagc aaaaacgaaa tgcaacgtta ctttacaatt gcaagtatag
    18961 ttggtgttat agttgttgta ggagtaattt tattactttc tgaccaactt agagcaactg
    19021 gtgataatgt acaatttgca ttaccacaag caaacttaat gtcaacatta acttctggta
    19081 ttatgtcagg tagtttacct tgggttatga ttattgttgg agtatttatg gcaatagttc
    19141 tatatgcatt aaatttacca ataatgacta tagctatagg attctattta ccaattgcaa
    19201 caacttctat aatattagtt ggagcattaa ttcgtttatt tgttgaatta gtatctaaaa
    19261 ctgaaaaaga aaaagaagtt aaagtttcta atggtataag tttatcttct ggtcttgttg
    19321 ctggtggttc tatcattggt ttaataggta taatattaca agtaactgga gttgtaactc
    19381 caaaggtacc aagtggattt gctgcaacaa attcaatggc aattgcttta ttagtagttt
    19441 tagttgtgct tacaacatta cctattgttt tatctaaagt aaaaaataat gagcaagagt
    19501 aatgatgttt tagatattgt atttaaaatt actgatggac ttgaatatga agtagctgaa
    19561 aatagtgttg ttactatact tgaaaaacag gaccataaaa ttcaaaagtt ttttagaaag
    19621 ttaaaagcta gaatacctaa atacaaaaaa atagaactag atgaatattc cagttttgtt
    19681 tttttacaaa ttgatggtca aaggactgtt agagaaatag gcaagaacct agaagctaaa
    19741 tatggagaag aatctcatcc actttatgaa aggttgttag tatttttaaa tcatattgaa
    19801 gttaattgtc actatattga aagactggat atatagatat aataagaatt attatagata
    19861 tattaaagca tataaattag aaggtaatta tgaaagaggc tgtctcaaaa tagagataag
    19921 cctcttttgt attgttgaaa tgatgaaaac atatttgatt tctcataaaa aagagtgtct
    19981 cataaaacta aaaagttcat tttgagacac tacctttaaa atatttataa agtatagatt
    20041 taaagcaaaa cttattaaac ttaagattat tttaatatca cttattatta gctatttcat
    20101 cagctagatt atttaaatat tcccatcttt catatttgta ttcaagttcc ttttctagtt
    20161 tagatttctc atctaaaatt tcttgaagtt tagtaaaatc agttgaaaat tttgcagtac
    20221 tttcatcaag tgttgcaatt ttagattcga gtttttctat atcagagtca atattttcaa
    20281 actccctttg ttcattatat gagaacttaa gctttttatc atttttaggt ttttcttttt
    20341 ttagggtagt agagtcatct tttttaactg attccctttt agattcttca aattcaattc
    20401 cttgaatctc tctataaata agataatcac tgtaattacc tgtatagata tgaatttttc
    20461 cattaccttc atatgcaaat attttattac aaattctatc taagaaatat ctatcatgtg
    20521 atactgtaac tactacacct ttaaattcgt ctaggtattc ttctaatatt ttaagcgttt
    20581 caatatctaa atcatttgta ggttcatcta aaagaagcac attaggagct gtcataagga
    20641 ttttaagaag atgtagtctt cttctttcac caccagataa tttacctatc aaagtatatt
    20701 gcatagtacc attaaaaagg aatctttcac acatttgaga agctgtaatc ctagtaccat
    20761 ctgaagtttc tatatagtca ctagcttctt ttacatagtc tatagccctc atatcaatat
    20821 tcatatgaga gtcgtcttga gaaaaacaac ctattttaac tgtttctcca attgaaatat
    20881 atccactatc aggctctatt tctccattaa gtatatttat aagggtagat ttacccatac
    20941 catttttgcc tattatacca attctatctg tacgagttaa agtgtattct aaattatcta
    21001 taacagtatt ttcttcaaat gatttagata tattgtgtat ttctattatt ttatttccaa
    21061 gtctacttga tgcaacagat atatcgagat tttcatcagg tttaaaagaa tctctattca
    21121 taagttcatc aaatctttgt aaacgagctt tttgtttagt gctacgagcc tgagctcctc
    21181 gttttaccca cgcaagctct gttcttatta aattttgtct tttatcttca atacttgctt
    21241 ctagcgaaag tctttccatt ttcttttcca aaaatattga ataattccca tcataactga
    21301 ataatctacc cttgtccagt tcaataattc tatttgtaac acggtctaaa aagtatctat
    21361 catgagttat cataagcaat gagccttttc ttgaattaag atattcctca agccaatcta
    21421 tagtatcatt gtctagatgg tttgtaggct catctagtat caaaaactca caaggtgtaa
    21481 taagggctga tgccagagag actctctttc tttgcccacc agaaagttct gctatttttt
    21541 gattaaagtc atttatacca agctttgtaa gtactgactt agcttcactt tctaaatccc
    21601 aaagatttaa ggcatctatt ttttcgtgta ctgataataa ttttttattt aattcctctg
    21661 taaagtttga attaagtttt tctaggattt cttgatattc accaagtagc tttaattctt
    21721 cagaagttcc attaaatact tgttcaagta cagttgaatt ttcattgtaa gttggatttt
    21781 gaggtagata ttctatccta ataccattag tttttgttat attaccagtc tcactttctt
    21841 caactcctgc aataattttg agtaaggtag atttaccagt accatttaca ccaataagtc
    21901 ctattttttc cccctcattt attccaagag atatattttc tagtaatttt ttttcagaat
    21961 agcttttact tatattttcc aatgtcatta aattcatata attcaccttt atttctaatc
    22021 tattattttt caaaatgttg ataatctggg tttttccaat agccacccca actccaacct
    22081 ctgcttacaa aagcattata acaatcatca ccttttttta tcattccaaa ttttgtgcta
    22141 gacctatcag cataaactgt acttatttta ggtgtaacat catttccaga aacttgagga
    22201 ttctgtaatg ggtttatatc tatagcacgt cctttaccat gatttgaaac tacatttgta
    22261 ccagctatag ttctataaca aaatgatgag gtattattat cagacataga tttttcatct
    22321 acagcatcat agtcatctat taatttaatt ttttcaattg gatatttttt ttcgtataat
    22381 tccttaaaaa tatccactac ttctggagct agttcactgt ttactatcat ttctccttga
    22441 tgagtttttt catcaaatcc ataatatgta agttttaaat aagatagact atcaaagctt
    22501 ataggttcat cagtaggcat agatttacca atcattttat tttttatatt atctggtatt
    22561 gaagaatatg aaaaagtagg cttgatatta ttatcttcct gctttttggt agagctttga
    22621 ttatttttag aatcatctgt tgtattattt ttattctgca tagtttcttg actctttata
    22681 ttaccttttt tgttgggatt agagaacagt atctcatata tagataataa agcaagaaat
    22741 aatacagtta ttacaatcgt taattttaat ttattaattt ttcttcgttt tgacttttta
    22801 ctcatattat cccccagtat tagattcata taaactatat atattatacc tcataaaaat
    22861 aaactaggct aacaatattt agttaaccta gaacatttca tttatagttt ctccaattat
    22921 ttttattcca atttcaatgt cttcaaaaga caacctagaa aatccgagtc ttaaagtatc
    22981 aaaaccagag ttatctgtaa aaaataaatc tccaggcata aatataacat ttttttcata
    23041 acatttttct aataaaactc tagtatctat attttttagt tttataaata tatgcaaacc
    23101 tccatcaccc aagatatatt ctgtttttat atattttttt atacatttat atgcaaagtt
    23161 aaatttgtcc ccatagaatt tacgtatttt tttaatgtat ttttcaaaac ctccattact
    23221 taaataatca aataaaattc cttggtctaa aaaggataca tgtatgtttc tgcacctttt
    23281 tacactttct agtttattga ttactttttt atctgcaaat atccatccaa ttctcattcc
    23341 aggaaataaa atttttgaaa aactaccaat atatacgacc ccattattca tattatctag
    23401 ggaacagata ggaaatatat gagaactatt gtagagtaat tcttcattaa atccatcttc
    23461 tataattgct atattgtatt ttttcataag attatagaat ttgtacctat tttcaggagt
    23521 cattacaatt cctgtaggat tgtgataaga gggtgtaatg tatgcaaact ttattttatt
    23581 tttatactta actaaattgt cttctagcat attgaagtcc aaaccatctt catttatatc
    23641 tacacctaag atattgaggt tatgagattt cataattttt atagcagtat tatgagtagg
    23701 attttcacat attatgtagt ctttttcttg ggtaaaagat gacataataa tatcaaagcc
    23761 ttctgtgaat ccatttgtta ttagtatatc tttattactt atatcagcac ccttgtttgt
    23821 catatattct agtaaatagt caataagggg cttataacct tgtgcatatc catagttgag
    23881 taatttatgt ccttctagtg aaattctatt taaaaaggat ttttttaatt cttccatgtc
    23941 gaataaatct ccatctggag aaatactttt aaaggaaatc aggtcagagc tccaaggaat
    24001 ttcacttttt ataatgtcca attcattggc ttttttagag tatgtattct ctaaacaatc
    24061 ccagtttaat gaccatgtag tatttgaaga cttattcttt gatttaacaa atgtaccttt
    24121 tcctgaaata gagtagatta atccttctga ttttaattct tcataagtta aaactacaga
    24181 atttcggcta acttgtaaaa gctgactaag ctcccttgta gaagggagct tactattgtc
    24241 tggaattagt ccttttgata tcatatcact tatataattt ttaagttgta tataaattgg
    24301 ttcattatca tttagtttta gatttgaaaa tatcaaatta ccacctcaaa ttatttcatc
    24361 taatttaaag agtattctat aagtttatct aatagttcac tgtacttaat acctctagca
    24421 gcagcacttc taggaattaa actagtttga gtcattccag gaagagtatt tagttcaaga
    24481 acatatggaa taccttcact tataatcata tcaactctta catatgcttt gcaattaaaa
    24541 attttccaac aagtctcaga aatttcgttt actctttttt gaagttcttc atctaagtaa
    24601 acaacttctt cctttgcacc tttttcaact gagtatttag cttcataatc aaagaatcct
    24661 gaatcagatt ttatagatat tgtagggaac acttctccat ttaatatgaa tgaagtatat
    24721 tctccacctg gtatatattt ttcaatcatt acaaattcgt caacttccag accttttcta
    24781 actgcttctt caacttcatc cttagaatga ataaagaatg tggctacact tgaacctcct
    24841 gagtttggtt ttataaatac tggataacct atattatcta tttcatcata gtcgatttca
    24901 tcaactgatt ttactaaaac ccatggagca gtaggtaggt ttgaatctct aagcattttt
    24961 ttagttatat ttttatccat acacatccca ctgcaaagag gtccacatcc agagtatggt
    25021 atatccatag tctcaagtat tgattgaata catccatctt caccaaattt tccatgtaaa
    25081 gcaagtattg caaaatctat atcttctggt atctttgtaa atatatcttt tttatcatct
    25141 attattactt ttacaacttc atatttattt ttatcaaggt tattatatat ctcttttcct
    25201 gaatttaaag aaacttctct ctctgatgat attccaccca ttataactgc tattttcata
    25261 ttatacactc ctattttaat ttttattatt ttaaagtaaa ctttttattt acatacttaa
    25321 ttatatgaat taacagttga ggatgaaagt accactatat acgaagttta tattatgtgt
    25381 ggttttagaa taataatcta gtttaaagat atgtttaaat gattttttaa ctttatttat
    25441 gttattattt ttgctaaaat tattgttatg aacttaagac tagtcttaat ggctataaat
    25501 caaggagtga taaattttga aagctgaaat aataagtgta ggaacagaaa tattattggg
    25561 agatatagta aatacaaatt ctcaattttt agctaaagaa cttgcatcat tgggaataga
    25621 agtataccat cagagtactg ttggagataa caaacaaaga cttttagaat gttttgatga
    25681 aagcttaaag agaagtgatt ttgtaattac tacaggtggt cttgggccaa ctggtgatga
    25741 tatgacaaaa gaaactgctg cagaatactt tggtcagaaa ttagaactac ataaaccttc
    25801 actagaggtt ttggaatctt tctttgttaa aacaggaaaa aaaatggccg aaaataatat
    25861 gaaacaagtg tactttccaa aagatgctat agtactaaaa aataataatg gtacagctcc
    25921 aggggcaatt ttaaaaaaag atggtaaatt tataatagta ttgcctggac ctccaagaga
    25981 aatgaaagct atgtttaatg agagtgtcaa accttactta caacaattca caaatgaaat
    26041 gctagtatct aagactttaa gattatatgg aataggtgag tcaaatctag aattagaaat
    26101 tctcgatata attaatgagc agactaatcc aacagttgct ttatatgcaa aagaattaga
    26161 agttacaata agaataactg caaaggctga aaatgaaaga gaagctttta aattaataaa
    26221 accagtagaa gaaaaaataa aaagtagagt tggtaaatat gtatatactg aaggagatat
    26281 ttccatatct gaaggggaga ctgctttaga agatgctgtt tctaagttac ttgtagaaaa
    26341 aaatctaaca atagcagtgg ctgaatcttg tactggaggt ttggtttctt catctcttat
    26401 aaactatcca ggaatatctt ctgtatttct tgaaggatgt gtaacatatt ctaatgattc
    26461 taaaatgaaa agattaggag taaaaagaga aactttggaa gagtttggag cagtaagtga
    26521 gcaaacagct atagaaatgg ctgagggcgt tgctaaagga cttaaagcta atataggtat
    26581 atccacaact ggagtagctg gacctggtgg aggaaccaaa gagaagcctg tgggactagt
    26641 ttatactgca atatatataa atggaaaaac aatagtaaag aaaaatatat ttaatgggga
    26701 tagaagaaag ataagattaa gagctactag agatttactt aatgaattga gaatacaatt
    26761 agaaaagctt taagttgtta tgatattgga tatatttgaa gtgttactat aatataaaat
    26821 tgtttttgga ggcttttaat ttatgataaa acatatattt tgtgatttag atggaactct
    26881 atatgaaaat ggaaccataa caaaagaaga tatagtagca attgaagaaa tagaaaaaaa
    26941 gggagtacag tttaatgttg ctacaggtag aatttttaaa caagcacata atattatcaa
    27001 agatagttta gatatgaatg gatattatgt atgtgaaaat ggttcattta tatatgataa
    27061 agattataat gtaattttta aaagaacgat agatgataat ttagtaaaaa aagtcattga
    27121 tagatttgaa tcaagtgatg cacaacttta ttttaaatat aagggtgatg taattgtggg
    27181 tagtgatacc actgctttta gacattattc tagtgacttt atagttgacc cagattttga
    27241 aaaaagaagt agttttgata atcttatagg aaatataggc gtatgttctg aaaatttgga
    27301 agagttatcc agaatagaac tttatctaaa aagtgagttt agtgaagtat tagaaatata
    27361 tttttctgga acatatactt taaatatagt tcctaaaagt gtgtcaaaaa gaggaagtat
    27421 agagcatgta ataaaaacgt taaatgtaag tccagatgaa gttgctacta ttggggattc
    27481 tccaaatgat atttgcatgt tagaaggatt taaatatagt tttgcaatgt ctaaggcaag
    27541 ggaagatgta aagcaaagtg caaattatgt agtagatagt gtaaaaagtg ccatagatgt
    27601 tataatggag ataaatagtt agtataaaaa atgactgtcc tagagaagta taaaattata
    27661 ttctatagag cagtcatttt aattatattc aattataagt ttatataaat ttaaaaattc
    27721 ttaagttaac cgcaatttgc tatatgatat gattttattg tattagataa aatagagtca
    27781 agtatataat ttatgacact attacaagta taagctgaaa attttaaatc attcatataa
    27841 tcagttttct ttttgtaatc actataaagt ttagtaaaaa acttctcaaa actactattt
    27901 atagtaccac atttaatttt agataaatca acatcttttg caaatgctcc aagttctctt
    27961 tcataagtaa catgtttatt taagctatgc ttaaattccc aacgttcact gtgagctaca
    28021 cagaaaaaat cacataaaaa atgacatatg actcctagtt cctgactaag tctacttact
    28081 gaaaagtatt tagaaagact atctatatta agactgcata gatagtttat tttagaaaca
    28141 atcatattaa atgactcatc taaataatgt ttttttaatt tatatttaga aaaagcatca
    28201 ggctttatat tgccatatat aaaatttttt tcgcttataa agaatgtttt attttcatct
    28261 atattatcaa gtattgattt tgccataata aaatgggtat tcattaacat atttacacct
    28321 cgtatagttt attagactta ttaaatatat tattatgata tgatatttat gataaaagtt
    28381 taatggtaaa tatatgaaac taagtcaaca gtatttattt aaataatttc aaccatattc
    28441 ataataacag taaatggcca aatttacaat atgtaaatag tgttaagttt ttatttatca
    28501 aatttaaaat taagtattag tatactttct aattaaccta ttggctttag actgtgagat
    28561 ttcaaggtgt ttggctactt tataagaact accaaatttt tcataagcat ccaaaattat
    28621 atttttttca tactctttaa ttaaggtgtc aaaattttta tttttataat tagtagattt
    28681 tgagtcagat ataataacct tattaatttg ggatatatgt gttttatttc cattattggt
    28741 aatatgtata ttttttgtaa gaatgtttgg taagtcatct attgttatat gattatctgt
    28801 acaagtaagc aagatttttt gaattatatt ttgtaattct cttatattgc ctgggaatct
    28861 ataatttgca atagcatcca tagcttcttt agaaatagtt ttatttaggt tgaagtcact
    28921 tgagtaccta ttaaaataat attttataat ttccactaga ttatcccttc tttctctaag
    28981 aggaggcaat tttatttcta taacatttaa tctatagtat aaatcttctc taaacttttt
    29041 ttcttttact tttgaaacta aatcagcatt tgtagcagat ataattctta tatcaacctt
    29101 tttatttttt aagctcccaa ctggagtaaa agttctatcc tgtattaatt gcaaaaattt
    29161 tgcctgtaga ttttgtggta tatctcctat ttcatctaaa aaaagagttc ctccattagc
    29221 taattctacg agcccaactt tacctttagt acttgcacct gtaaaagcac ctgaagtata
    29281 tccaaaaagt tcagattcaa gaagttgtgg agatatagtt gcacaattta ttgttataaa
    29341 tgggccatta gatctattgc tgttgtggtg tatatatcta gcaagtgagc tttttcctgt
    29401 tccagattca cccaataaaa gtacacttac atccgtttta gaaacatgat ttactgtgtt
    29461 tatgattggt ctcatcacta ttccgtccat aattacagta tcttcaattc taagtgcagt
    29521 tctgtatagt gtttctacct cagatttata tttttttact tgcttttttg tgtcttcaag
    29581 tttatttttt atattattaa gttcagttat atctctacaa ttttcaacag taaattctaa
    29641 gtttccattt tcatcaaata taggggtagc agttatgata agctttttcc ctgtttgagt
    29701 atcttgttcc agtgaaaatt tagtctttgt ttttaataca attggtatgg ggctttttgt
    29761 tgaatatcca gagtcttcta aaaaagaaac attttttcct aaaatatctt ctttttttag
    29821 accataattt tcttcaaatg ctttattaca atatattatt attcctttat catcagaaac
    29881 acatatttca tcatgggaag cttctaatat tttttgataa aattccaaat ctaaattcat
    29941 ttttatattt tcctcctaaa tttttaatga tatattaaaa taggtttaat tgattaatat
    30001 acttaaaatt tgatatttat ataagtttca gtctatttta actttaaata agttatatat
    30061 aactcatttt acaattaaaa taagtctaaa tcaacttaaa gtaagcgaaa aataaatata
    30121 agaaaattga aaacttatta gtattattgg aaagactagg gttttagagt cgatatatat
    30181 tggtttaaga tggcataatt tttgcttaaa aagaagagtg taaattgact aaataaaaat
    30241 agaagggaat ttaaaatggg agaaaaaagt aaagggtatg tatttatagc aatagcaggg
    30301 ttattatggg ctacacttgg attatttgga aagtttttaa tgggaaatgg actgacatca
    30361 gaacaagtag catttacaag attgtttttt ggatttatag ttttaggcgt atattcatct
    30421 ataaggacac cacaaatctt aaagataagc aaaaaaggaa ttatatattc agtaataata
    30481 ggaattatat gtcaggcaat gtttaattta tgttatttta aagcaattga cattgcaggt
    30541 gtatcaattg cagcagtttt actatataca tcaccactgt ttttagcaat attttcaaaa
    30601 gtatgttata aagaaaatat tactagaagc aaattatttt cacttattct ttgttttatt
    30661 ggtgcaatta tggcagtgac tggtggacgt ttggattttc aaggtttaaa cgcttttgga
    30721 cttttacttg gggtattatc agcaatagca tatgcactta tgccaactat tagtaaaaat
    30781 gctttaaagg aattttcaag ttcaacaata ttagtataca gtttcttatt tggagctata
    30841 tttatgattc cttcttcgag accttgggaa atcttaaatt atgctaagga tttagatgtt
    30901 ttatcttgta tgctaatgtt aggcattgtt ccagctgcac ttgcatatat tttttatgca
    30961 gcaggtatat ctaaaggtgt agagttgtca gttgcaggtg ttgtggcatc agtagagtta
    31021 gttggttcag taataattgg ttgcactata ttaggagaaa gtttttcctt agggaagtta
    31081 tttggagtga tgttgatgtt aatatctgca gtagtagcac ttaatctttc ttatgatgaa
    31141 ataagaatat tttataaatc caacaagtta aagcagattg aaaaaactga aagtatttag
    31201 aattatatac ttaaaaagga tttattggta tacattttag aatccaacaa aaattatacc
    31261 aaattatatt tggtattaaa aaataactat ttataaattt tatagaataa agtggacagt
    31321 acaaataaaa tataagtaga tacagtttag actatctatt ttttattata tattgtataa
    31381 atgtacaaat taatattttg ttttgtaaat actaaataac tatagaaagg actatattta
    31441 tataaccatt tctatagtta ttattttgta ttaaacaaac ttacagccct gataagaatt
    31501 tctagccttt aaagtatcat caatttgatt taaaatttcc cattttttta ggctccatat
    31561 aggacctact agttcatccc tcttaccatc accagtgagt ctatggatta tcatagtttc
    31621 aggaagtatt tctaattggt cacagacaag attgatatat tcatcttgtt ccataagagt
    31681 taacatacca ttttgtaaca tcttttccat aggagtatct tttataacat gcaatagatg
    31741 gatttttata ccgtctacac ccattttacc aactgccttt gctgtttcca tcatcatatt
    31801 ataatcttca ccaggaagac catttataat gtgtacgaca acctttatat ttttagactt
    31861 taatttttca acaccttcaa caaatgtttt ataatcatga cctctattta taattttaga
    31921 tgtagaatca tgaattgttt gaagtccaag ttcaacccat aaattggttc ttttatttag
    31981 ttcagataag tattctacaa catcatctgg caaacagtca ggtcttgtgg atattgaaag
    32041 acctatgaca tcttcaagtt ctaatatagt ctcatatttt tcttttaaaa cttcaagagg
    32101 agcatacgta tttgtatagg cttgaaagta gccaatatac tttgcatgag gccatttttt
    32161 taacatcatt tcttttatat tataaaattg actgataaga ttatctttag ggtttccagc
    32221 aaaatcacct gagccttctt tgctacagta ggtacatccg ccataaccaa gacttccatc
    32281 aatgtttggg caggtaaatc ctgcatttat agatacctta aaaacttttt ctccaaaagt
    32341 atttctaaga tagtaattcc aagtatgata tcttttatca tcaaaagcat atttaaattt
    32401 atccaaaaaa atcacctatt tctaataaaa ttgtacgctg agcttgtaca aaaaacatat
    32461 taccacaaat agataactta aacaaattct attttaagta atataaaaat acagcgagaa
    32521 aataattaat attttctcgc tgtatttcca gagaaaaata gaactagtcc aaataatatt
    32581 atgaaaaaag acattatcga tactataaat ctagataaaa aaagtggaga taatataagg
    32641 catataccaa atagaatctt aactatattc caagttgtaa atttagaata aggattattc
    32701 ttactattta caagtgctac tagttgatta aagattaaat aaaaccctaa aaataatgaa
    32761 aatatgcttt gtattttttg aggaaataca aaaagtaata tacatgcaat tattagaacc
    32821 cattggatat taatagaggg tttaatttct tctttagtag catatctttt tagttcatta
    32881 atatttttta aatttaaata tgcaaagaat aaaagtgcaa taccaaaagt ccaagaaatt
    32941 aattttatac ctaatgtctt aaatgtaagg cataataatc ctaagataat gagcaatact
    33001 cccattaaca taaatttagt agcattttct tttttattaa agttattaaa atcgatatta
    33061 aaatttgtgt acataaaatc acctccaaaa tcgatagaac aattataaca gaaattcata
    33121 tccaaataaa gcctagtgta taaaaattaa actatactta gtaattaatt aataggttgt
    33181 atcatagact tctattaata gaattatata gaaggaggtt tgtccatgac aaaattatgg
    33241 gcaataatat ttgcattatt agcaggtggt tctacagctc ttgaagcttt tataaatgga
    33301 gaactaggaa aaagtacaac agcattagtg gccaccttta taagtttaac tgtaggagca
    33361 atattttttt tggcaagtat aatagttaca ggtgacttaa agtgtctagt atctataaat
    33421 ggttttaatt ataagttgtt attaggagga gtatttggag gtttaataat atattttaca
    33481 gtaaaggcaa ttccaaattt aggagtatca aatacactta tattaacact tgtatcccaa
    33541 gttttagttg gattttttat tgatagcgta attttgggac aagaaacaat gcatttatac
    33601 aaatacatag gggttatact tttaatatta gggacattct ttatagtgaa ttaacaattt
    33661 aatataattt gctatgcaac tcaaaaattt ggtataatta tgttaataaa acaaagttag
    33721 atgaaagagg tgttaaaatt ggctgatgaa aaatttgtaa tgtcatttag tggtggtaag
    33781 gatagtatat tagcattaaa tcgaatgttg aaaaaaggat ataagcctgt ggcactcttg
    33841 acaactataa gtgaagaaca tggaaagtca tggacacata atttagaata taatatgctt
    33901 aagcaagtaa gtagcaatat aggattacct ttattagttg ctgagtgtgg tgtagaagga
    33961 tatgaagaga gctttgagag ggctttaata aaagctaaaa acatgggagc aacgatatgt
    34021 gcatatggag atatagatat agaaagtcat agaaaatggg atactgatag atgtgaagca
    34081 gttggtatga aagtagattt accattatgg caagagagta gagaggattt agtatatgaa
    34141 tttatagaca gtggattttg tagtgttgta acaaaagtaa acttaaagca cttaggagag
    34201 gaatttttag gaaaaaaatt aactagagaa cttgtggaga agataaaaaa tgctggtgca
    34261 gacccatgtg gagaacatgg agagtatcat acatttgtcg tagatggtcc aatatttaaa
    34321 aaacctgtag aatatgaagt aaaaggaact ttaataaaag atggctatgg atatttagaa
    34381 ataaaataat tacataaata aaatacctat tatataatcc taatttttgc aaaggtctaa
    34441 tatttgcaag attgtataat aggtattttt tatttagata ttttcatata ttgctactct
    34501 tatcatctaa gcaaccttat ttagattaga gtctaatgta gaaaaattct tttttagaaa
    34561 aactaatgat aaaataagac atataaactc agaaacaata gtagtcatcc atattccttg
    34621 tccaccaaat attagtatca ttgaaattag acttaaaaca actattacaa gccctctacc
    34681 aagagatata acagtagaaa ttgatggttt ttctactgat acaaagaatc cagaaattat
    34741 tacattaatt cctaccaata agaaagatat agagaatatt ttaaatacac gaactccttc
    34801 attgaatagc attgtttctt ctgggtgtat aaataaagag actattggtc ctgaaaaagc
    34861 catacaaatt gcaaatacag caactgatgc tattattgtg gcttttatcc ccatttttaa
    34921 taattttttt acattatcta tatttcctgc accataataa aagctagtaa gtggttgcat
    34981 accttgagtt atacccatca tagtcataag tactaatgta tttatataat ttatgacact
    35041 ataataaata agagcatttt ctccaatata tttagtaaga cttaagttaa aaagtaaaac
    35101 aactatacca caacttaatt cagtagttga atcaggaaaa cctataaata caatttttct
    35161 aagtgtctta aaatcaattc taaattttga aaaattcaaa gtagagtttt ttcttaaaaa
    35221 atgtattaag aagaaaatag tagaaaagac ttgagacaaa acagttgcaa taccagcacc
    35281 ttttacaccc catccaaatt caattacaaa caagtaatca agtatgatgt ttgtaagagc
    35341 agatattatt acaccaacag tagctaatat tggaaagcca tcagttttta ttataacttc
    35401 taaagaataa gacactataa agaatccgtt aaaaaatatt attattccta aataatcttt
    35461 aaccatacca attgtagatt ctgtagctcc taaaaatagt gctaatctat ctaaattaaa
    35521 aaaagttata gctaatataa ttattgataa tataattatt gatactaagt taaatgaaaa
    35581 gacttcgtta gcagatttaa tatctttttt ccccagatag attgcaatta tggtagatgc
    35641 accagttgaa aataatagag aaactgcaaa tataaaatta ataaaaggca ttgatatatt
    35701 gacagcagct aaagccaatt ctccaactcc tttacttaca aatattccat caaccattgt
    35761 atatagtgag aaaacccaca ttgcaactac agatggtgct aggtatttaa aaaatctttt
    35821 acctagactc atgttttcgt ttatagatat ttttgtatcc atttattatc actccctgta
    35881 aatctattat aaaattacga aactattaat taataatttt atttctaata taaatgataa
    35941 actatggtat aataccaatg tcaacagcaa atagtatata attataaatt ttattaaata
    36001 atagtgattt taagggtctt ataaatatat cttatttata tattactgac atttaactga
    36061 caaataggaa aaaggtactt taagtgacat ttaactgaca aatggaaaaa aagcactttg
    36121 agtgacattt aattgacata aaactgaaca ttatttgaat agtattttgc ttatattttc
    36181 agatgtttct ttatccattt catctaataa gtgagaatat cgaatattta ttcatagcta
    36241 tttttatatc tgcatgtact aatattttat aaatagcttt tatgttagta cctgaaagta
    36301 atatcaaagt cgcatttgca tgtattaatt catgtacttt tatatctcta ggattattat
    36361 ctcttaatag ttttttaaaa tgaacaacaa aagttggttg aactcacggg ttgaatcttt
    36421 tatttaagca tatcaaatca aattcatttt tcaatatacc ctgtaatttt aacttatttt
    36481 gttttaattt ttgcccttta agcaaactta taagttcatc aggagcaaat aattttcttt
    36541 tggatctagg gattttaggg tctttgaaga ttattttatt gtatacataa actaatgttt
    36601 gatttacatg tgagattttt tatcaaggtc tacatctgac catataagtg atacagcttc
    36661 accaaaacgt aatccaagag ttagtatcaa aaaaattggt atacctatag gaatattttt
    36721 tgatttttct aataataatt gagtttcttg ctttgtataa aacttagaag ttgaactatt
    36781 ttttttgatt ggggttgtaa caaaatcaca aatattttct gatatttctt ttaatctata
    36841 agattctttc aatactgatt ttataaaatc gacttggttt tttattgaat ttaacatgtg
    36901 ctcattgtat atgttattta tataagactg taaaatagaa ggcgttatat ctattaattt
    36961 catatgttat tggaaaatcg aataataata acttggtatt cgaaactttt gataaagtaa
    37021 taaaactaca tccaaatgca aaacctgttt ttcatagtga tagaggtttt cagtatacta
    37081 gtaaggtgtt tgaatcgaaa cttttagcac aaggtatgat acaaagtatg tcaagagctg
    37141 gtcgttgtat tgacaatgct cctatggaag gaatttgggg aattttaaag gtggaaatgt
    37201 actatttaag gaaatttgat acatatgaac aattagttaa aataatagag acttatatat
    37261 attactataa taattttaga tatcaaaaga gattaaattc tatgtctcca cttgaattta
    37321 ggaaatattt aagtacagag atagcataat ttattttaag tagctctaaa aaaatttaaa
    37381 tatctagggt tttatttgac gtgctctttt ttagatagta aactttaaaa tatagatatt
    37441 aattatatga atataataaa aaaagtacta atgagttaca ctagtacttt ataactactt
    37501 ttacatgttt taactgtata aaacagtggg tatagttcag caggagtgac tttagttttg
    37561 aactaaaaat caagttcaaa aagaataaac attagtattt gaacttcact ctacgtctaa
    37621 atagattgta gttcttcttg tttttattat accacaaatt ggtacagata ttcaaaaata
    37681 atatatttat gatataataa aaatgtaaat agttttgcag tgagcgatat ttgttacaaa
    37741 gtagggctta atgcttgaaa tataaggtgt tgagggtata tgataaactt tatcatttgc
    37801 actactcatg gttcactgca aatttaagag agttgcacat gtgtaagtat tgaaaatgcc
    37861 cagtttattt tggggtaaag ttttatatta actaagtggt atggaaagag gtttttcaga
    37921 agtatatgta cgtctatatt gtaaagtttt atattaacta agtggtatgt aaataaggaa
    37981 ggtggaagtc aatatgcatg ggtcaaatgg gttttatatt aactaagtgg tatgtaaata
    38041 cgaaattaga gtttgaaata tatgactaca taatagtttt atattaacta agtggtatgt
    38101 aaatttgtgt cacatcattg tcacttcttt agaatatcaa aagttttata ttaactaagt
    38161 ggtatgtaaa tttttaagca ggtgattaaa tgattaaaaa agtagaaaag ttttatatta
    38221 actaagtggt atgtaaataa taacttgtca atgtagttat atgcaacaac ttctatgttt
    38281 tatattaact aagtggtatg taaatgaatt atatattatg aaaaaatatg gagtcacaga
    38341 gggttttata ttaactaagt ggtatgtaaa tttgcaggtt ttccttatag tttgccatat
    38401 gttcctggtt ttatattaac taagtggtat gtaaattact ttagcactag taaaggtaga
    38461 attattaaag tgttttatat taactaagtg gtatgtaaat tcgaatgtga atggcacttc
    38521 tatttctcca acttttggtt ttatattaac taagtggtat gtaaatagta taatgttgaa
    38581 aagttagaga gtacaatcaa gaaattttac attaactaaa atgtatagaa taaaacatgt
    38641 atttatactt aaattctgta cctatataaa aaagtgaact ctgtcaacaa agcacttttt
    38701 tatatagata aattatcatt ttattttaag atagaagata ctaatgctaa atgtttatca
    38761 ttagtatctg tatgtacata aaagtttaat tttttatata aatttgatct ttagaaaaat
    38821 gagcagtatc aataaatata ttgtctaaat tttttctagg aactagttga ctagctataa
    38881 gattagcttc aactctttga ttgttagact atgaaattaa atctaaaggt tcattcttgg
    38941 tcgtataaat agctttatca ttcgtatgta ctataacaat ttttgccatc tgcttttgat
    39001 agataaagag ctttatcagc tttagaaaat aaatctttat ataatttagt tgaatcatca
    39061 gtgaaggcaa taccaatact taatgttatt ttatgattgt cctttacttt tattttactt
    39121 gcatcattta aaatatcatt agtaattttt attattaaat cctttgttaa tgattttata
    39181 aatattgtaa attcatctcc accaattcga gcaactatgt ctttatcatg tgtgttatgt
    39241 aatagtattt ttgatacagc aactaaaact tcatttccct ctaaatgccc aaaagtatca
    39301 tttacagttt taaaatcatc aatatcaata actaatagag catcattatt tttggaatca
    39361 gaactacata gatactcttc aataagattt tgtgatactc tttgattata taattgtgtt
    39421 agtgggtcac gttgagcctg attaattaag ctttctaatt tagatttatc ttcagttatg
    39481 tcttttaaaa cgcctattgt tttgataggg ttatggtcac tatcatatat aactgaacat
    39541 tgaacacgaa tccatctcca ttcattttta gttaatgaat ttacttgtct tatctcaatt
    39601 gtttcacgaa gatttccatg aagtacttct agtagttttc caatatcatc aaggtgaata
    39661 attttaccag attctaatag tttggagtaa ttttttgttt caaaattttc taattcaatt
    39721 tttttgtcaa tttctacacg ttgatatttt ataagggtat cgttttcaat atcatactca
    39781 aacatgatat ctgtaatatt ttgcaaagca atacgatatc gctctctttc taattttagt
    39841 tcagattcta tgttttttag ttgggttatg tcagtaataa tactattaat ttttactatg
    39901 ccatcactat ttaacgattt cattccagaa tctaataccc acattaatgt tccatctttt
    39961 tttcggattc tatattcaga tgaatatgta ggtccttttg caaaacatcg ttcacaatct
    40021 tctagtgctt taggtaagtc tggtggatat acagcaccca ctgctgaacc accactcatt
    40081 tccatgaact catcatatgt gtatcctaat attttgggca atccctcatt gacatagaaa
    40141 aatgagtatg tactatcatc attgctacct tttaagccac cattgatatt atttgtaatc
    40201 atttcaataa cttctgtttt ttcctttagt aatctttgca aataatcata actttgagtt
    40261 ccaattttag tgggaaagta ttcatcaagt tgcatttcac cataagggtt tgatatgtgc
    40321 atgtgcttta ctaataattt tcctctatcc tcaattatct caaatgtaca acgttgattt
    40381 acctctaaaa tcatttgagc attttcttta gttcgaacag tatatcttcc taataccata
    40441 catctattct catcttcact tacagtttca aatacatcat tatttatatc acaagaagga
    40501 atttcatctt gacctgcata aaaataattt tttatttcat taaaagaagt gagaaattcc
    40561 tgttctcctg gtccaatcca tgtaacattt tcacaaaggt attttaatac ttcattgata
    40621 tctcgattta tataaaaact tgttataata tgttttgcta atttgggtgc tttttttagg
    40681 agtatgctca tgatattcct cctttctttt tgctattata ttttaattat attatacatc
    40741 aatatattta aattaaaagc tttgttaaac tatgatatct ttgtcaaata agttaatttt
    40801 aacaaaaaag aactgaaaca atatatgctt cagtacttaa tgtcaatatc ttgtatagat
    40861 acatatttat aaactaatgt acaagtttaa aaaaagaaag ataattttaa tttattttaa
    40921 aataacaatg ttaataatca tttttatcaa attttttctt gtttttctcc ttgtttttat
    40981 acattttttt atccaattca actattgtca attttaaatt tctttttact gtatcaacac
    41041 cataactaaa tgaaagatac ttatatttag agaatacttt taaaaaattt tctcttaagg
    41101 cttctagttg tgaataaaca taatttttgt caacatcact agagaatata acaataaatt
    41161 catctcctcc taatcttata atagaagtag aatttattaa ttctttattt aaaagtgaaa
    41221 caaaatcaca taaaatcttg tctcctacat tatgaccttc atagtcattt atcattttga
    41281 aattatctaa gtcaattagt acaatataat ctcctataaa tatgtcaaat ctatcattta
    41341 aatattttct attatatgct ccagttaatg ggtcagtaat tgagtagtga agatttctct
    41401 tattttcttc atagagtttt gttatatcat tggccatagt aattatgtag gttttttcat
    41461 caacatctac tttgtattct aaaacattcc aatagcttgt agtatctttt ttaaactcta
    41521 aaatccaatt tgaaatttta tcataaggat aatttttttc ccaatctaat tgaatattgt
    41581 gagaatccgt aaaaaattta gcaatattat cagggagttt cttagctatt tctatattag
    41641 tcattccaag tggattatct tgcatataac ttttaattaa attttttaaa ttatcattta
    41701 taaatataat tctgtagtta gaatctctta taattagaaa tcctggaaaa gttcttaaaa
    41761 aaatttcttt aaacatatta atttctccaa aataaaatac tttgtactag atttttgtta
    41821 aattttttat tttatttaca tctacataca taccttgggc aatcgacctt ccatcaattg
    41881 cagaaaggtt acattgtgta tacataactg tgacaatttg accatctcgt tttatgaatc
    41941 tatataaaaa tggttcaatt ttatttgtgt caatcatatc caaacttttt ttataaacaa
    42001 agtctaaatc atctggatga acaattgctc ttaaatttcc gcccatatca ccaaaaaact
    42061 gttcttttgt ataacctata atgtcaaaca tttcttcatt catataagta ataatcatat
    42121 caccaaataa atcgtagcga attataccta caggcaacat tgaataaaat tgtgataaaa
    42181 tattactttc atcaagatgt gtatttgtca ttttaggtat tgaatatgat tcaaattcat
    42241 ttatatagcc agcagtttta attaaatcca aatgtaattt tgctaagagt aatttatttt
    42301 ctgaaattat acaatttgca ctaaagtaga aattgctata tttttgatgt tcagaagctt
    42361 catctgataa aacggcttct ccactaatta ataatgaatc tgattcagta ttatgtgggg
    42421 ttatactttt gtaaagaata gaaaattttt tacctttaat atgtttttca aaatgttcta
    42481 ttactttagg aaaagatgta atttcagttt tgttgtaaaa atcaaaccat tgaatgtctt
    42541 cagtgacatt agctcttagt tttttcatat cttgcataat aaaaaatcca tcaaatatct
    42601 tctctattaa agattttcct atatcagaat attttataaa cattttctct gaattattct
    42661 ctttagatag taatttttca aattctttgc actccatggg ttttgcataa taataacctt
    42721 gggcgatatc acaatttata cttttaataa attctacatg ttgttctgtt tcaacacctt
    42781 cacatactgt aagcatccca agagctcttg caatgtttat tatagagtac aaaatggttg
    42841 tatttttaaa atcatttaaa ttatgagata aaaatatcct atcaattttg attatatcaa
    42901 aaggtagatt ttgcaacata ccaaaagatg aatatccaga accaaaatcg tccattgata
    42961 cagaaaaacc ataagaacga agttcagcta gttgttgaat aattgtattt gtattttcta
    43021 ttaatatatt ttctgttatt tcaatgctaa aatttgatgt agagacgctg tacttttcag
    43081 taataccttt tagcatactt ggaaaattag ggttaaaaaa atgaagacga gaaaagttac
    43141 aacaaattgg aacaaaaaca tcattgttat caatccatct tcttatattt ttacagttct
    43201 cttcaaataa atgcaaatct aattccgtta tcagaccaaa ctgttcaaat aaaggaataa
    43261 atttatctgg agtaagaagt cctttaatag ggtgtctcca tcttgcaagt gcctcacatc
    43321 caactatagt attatttttt atattataaa gtggttggta aaatacttca atttgtttag
    43381 tttgtagaac atgtgatatt tctgataaga actccttatc attttctaat ttattgtaaa
    43441 aactattatc tttaaaatca aatagaatgt atctattttt gccttcactt ttagcctttt
    43501 ttaaagcact atatacttgg ttgaaaagta tcaaagtagt catgtttggt tcaccattcc
    43561 agatagtgct gccgatacta aatgtaacat tggagtatat ttctttttca ttaggaagtc
    43621 ttatttttaa ttctcgggag ctctctataa tagttgcacc catattattt aactctttat
    43681 ttgatacatt tggaagtaca attaaaaaac cttctccttt taaataagcg actattccct
    43741 ttgattctgg aataaagtgc aataggtttt gagttagagt aattaataaa ttttctccga
    43801 ctagatatcc atatatatca ttaaataatt taaagttatc gatatttaat aacatgattc
    43861 ctatatgtat atttttcgaa atacagtcat caatatattg gtacataaat tctctgtttg
    43921 gtattttaga aaaaaaatct atttcattta aggtttttaa tttaaaattt ctactatcta
    43981 attccttaga taatacttct acctttttat ttaacatttt tatataatta tgattttgca
    44041 tggtattcct ccataagata ctttaaattt tgatgacaac tgtatttatg ctagaagtat
    44101 ttaaatgtta atttgtaaat aatttaaatg ctaaatttta aataagtatt taaaatgaat
    44161 atacattaag ttatttgctc caatg
//
LOCUS       DAELXX010000020        44020 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.020, whole genome shotgun sequence.
ACCESSION   DAELXX010000020 DAELXX010000000
VERSION     DAELXX010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 44020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44020
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.020"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            223..339
                     /locus_tag="KPH29_001050"
     CDS             223..339
                     /locus_tag="KPH29_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein"
                     /protein_id="HBG1466675.1"
                     /translation="MDDDINMIAIRPEDIRVEFNRDFKEIIDLKLRPYRYKN"
     gene            503..1570
                     /locus_tag="KPH29_001051"
     CDS             503..1570
                     /locus_tag="KPH29_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG1466676.1"
                     /translation="MKFKKLVSLGILSTLLLSVITGCSNVSSGSNLKSNNKTKGELTV
                     YTAIEEDSIDTYLATFKEKYPDIKLNVLRLSVGGITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNPAFKDDKKVPSWVGIDAWMTGITVNTKELKAKNISA
                     PNSYEDLIKPEYKDLISMPSPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            1762..2292
                     /gene="rnmV"
                     /locus_tag="KPH29_001052"
     CDS             1762..2292
                     /gene="rnmV"
                     /locus_tag="KPH29_001052"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBG1466677.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMQRIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            2463..3332
                     /gene="rsmA"
                     /locus_tag="KPH29_001053"
     CDS             2463..3332
                     /gene="rsmA"
                     /locus_tag="KPH29_001053"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBG1466678.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREILKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            3398..4669
                     /locus_tag="KPH29_001054"
     CDS             3398..4669
                     /locus_tag="KPH29_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466679.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDSDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEEYEELEEDEEIEDNEESDRDEDS
                     EEEYIIEEYDEDEYEDYWEDDEDIEEGELKARTEVEDSGDEEIEEFNNYKEIENYNRV
                     KVQEEYIDIEYDEEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGV
                     SQGESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMP
                     YLGYLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGST
                     GFLRYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            4820..5935
                     /locus_tag="KPH29_001055"
     CDS             4820..5935
                     /locus_tag="KPH29_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG1466680.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIVAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            6121..7017
                     /locus_tag="KPH29_001056"
     CDS             6121..7017
                     /locus_tag="KPH29_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG1466681.1"
                     /translation="METRYEEANKITIQSILWNVVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASIVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            7087..7884
                     /locus_tag="KPH29_001057"
     CDS             7087..7884
                     /locus_tag="KPH29_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466682.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(7940..9292)
                     /locus_tag="KPH29_001058"
     CDS             complement(7940..9292)
                     /locus_tag="KPH29_001058"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBG1466683.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            9511..10389
                     /gene="purR"
                     /locus_tag="KPH29_001059"
     CDS             9511..10389
                     /gene="purR"
                     /locus_tag="KPH29_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBG1466684.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            10545..10823
                     /gene="spoVG"
                     /locus_tag="KPH29_001060"
     CDS             10545..10823
                     /gene="spoVG"
                     /locus_tag="KPH29_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HBG1466685.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            11033..12412
                     /gene="glmU"
                     /locus_tag="KPH29_001061"
     CDS             11033..12412
                     /gene="glmU"
                     /locus_tag="KPH29_001061"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBG1466686.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEAEVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSNVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SVVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            12507..13457
                     /locus_tag="KPH29_001062"
     CDS             12507..13457
                     /locus_tag="KPH29_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HBG1466687.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
     regulatory      14297..14382
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            14544..15065
                     /locus_tag="KPH29_001063"
     CDS             14544..15065
                     /locus_tag="KPH29_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG1466688.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKIPVTPDGQTGLSVLETYMESLPDKADIGGKYKLIKVG
                     NKLVLQIGTNDEGVTLTEAQSAKLLSDIGENKIYTSVTADNLGNPLTSNTKVDNKVLY
                     IVLIDNTVMDSTK"
     gene            15216..16892
                     /locus_tag="KPH29_001064"
     CDS             15216..16892
                     /locus_tag="KPH29_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBG1466689.1"
                     /translation="MAKKVRIGDKLVEKGYITEDQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            16904..18112
                     /locus_tag="KPH29_001065"
     CDS             16904..18112
                     /locus_tag="KPH29_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBG1466690.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTSVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            18127..19839
                     /locus_tag="KPH29_001066"
     CDS             18127..19839
                     /locus_tag="KPH29_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HBG1466691.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETHDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLVLDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQIKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTTDK
                     SSNNETKSSTKSDSNGDKTDNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            19832..20740
                     /gene="pilO"
                     /locus_tag="KPH29_001067"
     CDS             19832..20740
                     /gene="pilO"
                     /locus_tag="KPH29_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HBG1466692.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVKEYEHFYGISTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEILKNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            20767..21333
                     /locus_tag="KPH29_001068"
     CDS             20767..21333
                     /locus_tag="KPH29_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG1466693.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKN
                     TEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            21370..21897
                     /locus_tag="KPH29_001069"
     CDS             21370..21897
                     /locus_tag="KPH29_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG1466694.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            21918..23456
                     /locus_tag="KPH29_001070"
     CDS             21918..23456
                     /locus_tag="KPH29_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466695.1"
                     /translation="MRKWNKFKSERGAALVLVLIVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRNISDE
                     DFNNQDEKNIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRDSILEICKNYTSGDISKINEYILKEDIDSTTLIEAKLVNNDILLKMFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYIWNSKWDTLENLLEILPNQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKDGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIVNFNLNIDTNVVGNYDIKDKILINHDISSY
                     KKVN"
     gene            23556..24638
                     /locus_tag="KPH29_001071"
     CDS             23556..24638
                     /locus_tag="KPH29_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBG1466696.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESIFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            24619..25401
                     /locus_tag="KPH29_001072"
     CDS             24619..25401
                     /locus_tag="KPH29_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBG1466697.1"
                     /translation="MDIIIVNLYVFIVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGILFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILIFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGLYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKGKEYNSVIPYGPFISIGALITILCGKQLLELYFSMVI"
     gene            25503..26153
                     /locus_tag="KPH29_001073"
     CDS             25503..26153
                     /locus_tag="KPH29_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBG1466698.1"
                     /translation="MEYIIIISLFFAHLINKSLCVFCDREKKNLYNTFFIYIITVVLS
                     VLIYLKFGISVECVQYMVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLAFFNI
                     AQFDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFI
                     LAFLFFIILYITKIFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYKFI"
     gene            26167..26727
                     /locus_tag="KPH29_001074"
     CDS             26167..26727
                     /locus_tag="KPH29_001074"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBG1466699.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAADAIDSIIRENIDMSMNKYNG"
     gene            26744..30130
                     /gene="mfd"
                     /locus_tag="KPH29_001075"
     CDS             26744..30130
                     /gene="mfd"
                     /locus_tag="KPH29_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBG1466700.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGAENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            30161..31156
                     /locus_tag="KPH29_001076"
     CDS             30161..31156
                     /locus_tag="KPH29_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG1466701.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            31280..31819
                     /gene="spoVT"
                     /locus_tag="KPH29_001077"
     CDS             31280..31819
                     /gene="spoVT"
                     /locus_tag="KPH29_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HBG1466702.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            31939..33552
                     /locus_tag="KPH29_001078"
     CDS             31939..33552
                     /locus_tag="KPH29_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBG1466703.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            33659..35107
                     /gene="mazG"
                     /locus_tag="KPH29_001079"
     CDS             33659..35107
                     /gene="mazG"
                     /locus_tag="KPH29_001079"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBG1466704.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            35196..35474
                     /locus_tag="KPH29_001080"
     CDS             35196..35474
                     /locus_tag="KPH29_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBG1466705.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            35546..35788
                     /locus_tag="KPH29_001081"
     CDS             35546..35788
                     /locus_tag="KPH29_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBG1466706.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYEILE"
     gene            35850..36104
                     /locus_tag="KPH29_001082"
     CDS             35850..36104
                     /locus_tag="KPH29_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HBG1466707.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            36105..36638
                     /locus_tag="KPH29_001083"
     CDS             36105..36638
                     /locus_tag="KPH29_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466708.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            36644..36928
                     /locus_tag="KPH29_001084"
     CDS             36644..36928
                     /locus_tag="KPH29_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBG1466709.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            37041..37961
                     /locus_tag="KPH29_001085"
     CDS             37041..37961
                     /locus_tag="KPH29_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HBG1466710.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIKFNKSENVGAL
                     RMTERFLKHDIIEDEEFEAMSSFIYDEIDKTLNHIKDRGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            38051..40423
                     /gene="spoIIE"
                     /locus_tag="KPH29_001086"
     CDS             38051..40423
                     /gene="spoIIE"
                     /locus_tag="KPH29_001086"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HBG1466711.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVEAIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNNEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            40774..42666
                     /gene="pepF"
                     /locus_tag="KPH29_001087"
     CDS             40774..42666
                     /gene="pepF"
                     /locus_tag="KPH29_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBG1466712.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAIAVMNNN
                     DTEDDAEKKQEVDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMSDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESVDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLESDNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            42780..43745
                     /gene="galU"
                     /locus_tag="KPH29_001088"
     CDS             42780..43745
                     /gene="galU"
                     /locus_tag="KPH29_001088"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBG1466713.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEARELEIQK"
BASE COUNT        17931 a         4598 c         8005 g        13486 t
ORIGIN      
        1 attataaact aaagtaaata aaatattgag cgattattaa aaagatatat atatatattt
       61 taatttgagc agtttgttat atattataaa aaaagagaaa aataaaaggt aaattgtttc
      121 tggataactt aaaattggaa gtaagaatat ttaactagag aagaattatt taagttcagg
      181 ttagctctag gagtagctga ttttatagga gcaatagatt ttatggatga tgatataaac
      241 atgatagcaa ttagaccaga agatataagg gtagaattta atagagattt caaagaaatt
      301 atagacctca aactcagacc gtatcgatat aaaaactaaa gctagatgat atagtgttgt
      361 atataggacc atcagctgtt atggaagtat tagtgctttt aacaaatata atagtaaaac
      421 ttttgtatga attgataata aaaaaattaa aaaccaagac ataaaaatgg caacaaaaag
      481 aagttgaata agggaggtaa aaatgaaatt taagaagtta gtctctttgg gaatattatc
      541 aacactttta ttgagtgtaa taactggatg tagtaatgtt tcatcaggtt caaacttaaa
      601 gtctaataat aagacaaaag gagagttaac agtttataca gcaattgagg aagatagtat
      661 agatacatac ttagctacat ttaaagagaa atatccagat attaaattga atgtacttag
      721 attatcagta ggaggtataa cagcaagatt attagcagaa aaagataatc ctaaagcaga
      781 tgtgttatgg ggtgttgcag ctacaagttt gataatttta gatgacaaag aaatgctaga
      841 gccatacgct ccgaaaggct ttgataaggt aaatcctgca tttaaagatg ataagaaagt
      901 tccttcttgg gttggaatag atgcttggat gactggtata acagtaaata ctaaagaatt
      961 aaaagctaaa aacatatcag ctcctaattc ttatgaagat ttaataaagc cagaatataa
     1021 agatcttata tctatgccta gtccagcttc ttcaggaaca ggatatttaa cagtatcaga
     1081 actcatacaa attatgggtg aagaaaaagc atggcagtat atggataaat tgcataaaaa
     1141 tattggtgta tacactcagt ctggttcagc gccagctata ttagcagctt caggagaata
     1201 tcccattggt atatcttttg gaaatagagg tataaagtta aaaggtgaag gttatccagt
     1261 agaagtagta tttccaaaag aaggttctgg atgggagata gaatcaaatg cacttgtgaa
     1321 aaaagataat ataaaagaag catcaaaagt atttttagat tgggctataa gtaaagatgc
     1381 aatgaatgag tattctaaga attttgcagt tactacaatt gacacgggaa atccggtgcc
     1441 agaaggattt ccaaagaatc cattagaaca gatgatagat aatgatttaa aatcagcagc
     1501 aagaaataga gaggctatac taaataaatg gatagaaaaa tatgatggaa agacagaaaa
     1561 agagagctaa gccttacaat aaaatttagt acaaaaagcg cctagaatat aagttttaca
     1621 tctaggctct tttgatatat ttatttaaaa tgcatatttc tatataaaaa taaatagttt
     1681 agtaccttga ttaaattaaa taaaaacctt aaaataaaga gatgtaaaga aaaactaaaa
     1741 acgaataaaa ggtgaattta tatgataaaa gaaataatag tagtagaggg tagagatgac
     1801 gttacagcag ttaagagagc aatagatgca gaacttataa ctacaggtgg atttggattt
     1861 ccaaaaggtg taatgcagcg tataaaagct gctaatgaaa gacgtggtgt tataatattt
     1921 acagacccag attttgcagg tgaaaaaata agaaaaaaaa tagcatcaga agttccagga
     1981 tgtaaacatg catttctacc aagagaggaa gctaaaaagg atggagatat aggtattgag
     2041 aatgctactc ctcaaagtat aatagcagct ctgaataaag ttagaactga aaatacagaa
     2101 aaaagagatg aatttaagca agttgactta attagaaatg gtcttatagg taatgaagaa
     2161 gcatcactta gaagagatgc acttggaaaa atacttggaa ttggatatgg aaatgcaaaa
     2221 caatttttaa atagattgaa taattatgga gttgaaagag aagaatttat agatgcatta
     2281 aaacaaatct aaacgtaatg tttaatgtat gaaaaatatg tatataaaat aaattgtgga
     2341 attacggtta atttaagata aattatttat aatatatacc ttattttaaa tcaatgtaaa
     2401 tacatataaa taagtataat taataaaatt aaatatcaaa ataagttaaa aaggagtaaa
     2461 taatggatag actatcttca cataatgcaa caaaggaagt agtacaaaaa tataatttta
     2521 aattttcaaa gtctttaggt caaaatttct tgatagatag taatataatt gataaaatct
     2581 tatctggagc tagaatcaca agaggagata atatcataga agtaggacct ggtataggaa
     2641 ctcttactcg tgagatgggt aaaatagcag aaaaagtagt ggctatagaa atcgatagaa
     2701 atttaattcc tatattgaaa gataccttgt cagatttaga taatacagag gttgtaaatc
     2761 aagatatact aaaagtagac atacaggaat tagtaaaaga taaattaaat ggtggaccag
     2821 ttaagcttgt agcaaatctt ccatattata taactactcc aatagttatg aaatttttag
     2881 aggaagacat acctgtaaca gacatagtag tcatggttca aaaagaagtt gcagatagaa
     2941 tgaatgctat tccgggtact aaagattatg gggcattatc tatagctgtt caatactatt
     3001 gtgatacaga gattgtggca aaagctccaa gacatatgtt tataccacaa ccaaatgtag
     3061 attccacagt tataggttta catgtaagag ataaaagaaa atatgatgtt cataatgaag
     3121 acatattttt taaaacagta aaagcatcct ttggacaaag aagaaaaact cttcttaatt
     3181 cgttgggagg tcttggtttt cttaataaag atgaaataag agaaatatta aaagaagcta
     3241 atatagacga aaaaagaagg ggtgaaactc ttagtataga agagttttca gttctttcaa
     3301 atataataaa tacaaaagta tcttctaaat agaaggtact ttttttaatt ttgttaacat
     3361 atattgttta gagaaactgt gttaaggggg gattgcagtg ggttctaaaa gaaagtttaa
     3421 aagagactat ataatactag aagctaaaga cataaacttt agatacaaag aacgagtact
     3481 gccaaaagct tttgcaaaag ttgaagtaaa tgatgaaaaa gcaattattg cattatatgt
     3541 agaaaatcta aaatatgtta aagatgggta taaagcagta gcaataaaag atgaccatgg
     3601 attaatagac ttaggaaata taattttaaa tgaacaagga agaggtgaat ttgttttaga
     3661 tttagaagat tctgatgatg agataaaagg tattgcatta ttgtatgaaa gaaatatacc
     3721 tttaattgga tttaagggaa ataagcttga aaattatgag gacatattat ttcaagaggt
     3781 agaggaagaa gaatatgaag aatatgaaga atatgaagag ttggaagaag acgaagagat
     3841 agaggataat gaggaaagtg atagagacga agatagtgaa gaagaatata ttatagaaga
     3901 atatgatgaa gatgagtatg aagactattg ggaagatgat gaagatattg aagaaggaga
     3961 gttaaaagca agaacggaag tagaagattc tggtgatgag gaaatagaag agttcaataa
     4021 ttataaggaa atagaaaatt ataatagggt taaagtacag gaagaatata tagacataga
     4081 atatgatgag gaagaagagg aagaagaaga agtttttgaa gtaaatagag aagaagatag
     4141 gtttgtttcc tcaaaaccta ctaaatcaaa gaaagttcaa aaagatcaga ataatattga
     4201 aaaaataaaa agagctaatg gagtaagtca aggagagagt ggaaataaac aaaccagtac
     4261 attacttatg ccaagacaaa taaaaaaagg tttgaaatta tttaaagaag taaaaccttt
     4321 tgtaacagat tatatagata agacaagatg gtggaaaata gagataaatc ctacaacact
     4381 gtgtggatat acaatgcctt atttaggata tttaaacgta ttgaattaca ctatgtatag
     4441 tgatgcagtt ttacattcat ataaatacag acactattta tttggtgttc agtatgatga
     4501 atataataag agaaaacatt atatttatgc tatcccagga aatagaaatg aacagccaga
     4561 caatggaagc acaggatttt taagatatca agcttgtgat aatagaacaa atagtttagg
     4621 atattggcta tgttttatag attctagaca aagaaatata gttaggtaaa aataataaaa
     4681 atgaaaaaat ttagactcaa ttcttatttt actgaaaaaa ttaatgaaat gtagtacaaa
     4741 actttaaaaa ctattgataa tatttaaata ttacactaca atatatcttg aaatacctat
     4801 tatattatgg aggaaaatta tgataaacga gcaaagaatt ttaaatgagt ttcttgaatt
     4861 agtacagata gatagtttat ctttaaaaga ggggaatgtt gctaaagtat tagttaaaaa
     4921 attagaagaa ataggttgtt cagtagtaat agacaatgct ggtgaaaaag caaatggaga
     4981 aactggtaat ataatagcta cattaaaagg aaataaagaa ggaaagaaaa tattatttag
     5041 ttcacatatg gatacagtaa ctccaggtat aggggtaaaa cctatagtgg atgaagcaaa
     5101 tggaataata aaaagtgatg gtactacaat attaggttct gatgataaag caggtatagc
     5161 agctatatta gaaggcttaa gatatataaa tgaaaataac atagaacata ctgatataca
     5221 agttgtattc tctatatgtg aggaatgtgg attagttgga gctaaaaatt tagactatgg
     5281 aaaaatagat tctgaatatg catttatatt agatagtggt ggttctccag gtgaaataat
     5341 agtaaaagct cctgcacaag atgtaataaa tgttaagata ttaggaaaga ctgctcatgc
     5401 aggtcttgaa cctgaagctg gaataagtgc tataatggta gctgccagag caatagaaaa
     5461 tatgaattta cttagaatag acgaagaaac aactgctaac ataggaatta taaacggtgg
     5521 aacagctaca aatatagtaa caggagaagt taatatagtt gcagaagcca gaagtctaaa
     5581 agaaaataaa ttagatgttc aaactaaaca catggtagag acttttgaaa aagctgcaaa
     5641 agattttgga gctcaaattg agatagatgt caatagagca tatacaccaa tagatgttag
     5701 tgaggattct gaaataataa aacttgcaaa gaaggctttt tcaaacttag gtatagaagg
     5761 tcatactgaa tctacaggag gaggtagcga tacaaatata ttaagtaaga atggtatcga
     5821 agctataact ttaggaattg gtatgaaaaa tgctcataca ctaagtgaac atatagctat
     5881 aaaagactta tatgactctg ctaaaatggt agtggagata ataaaggaag cataaattta
     5941 aataatagtg tataatatat atatcgccta gcttaagcta ggtgattttt cattttatag
     6001 aagattgata atctctattg aaagcaaaga gtatacattt gcatataaga gagtgtttaa
     6061 tttatgttca gatgtaggtt cttaataata tttagattgt ttcataaggg aggaataaat
     6121 atggaaacaa ggtatgaaga agcaaataaa ataacaatac aatcaatact ttggaatgta
     6181 gtattaacaa taattaaggt tatagctggt gttattggta attctagcgc tatgattgca
     6241 gatggattac attcagcatc agatattata agttctatag gagtactaat aggcaattat
     6301 gtatcttcta gacctggaga tagagagcac aattatggtc atgagaaagc agagacatta
     6361 gtatcatttg tgttatcaat attgcttatt ttcgtttcta taacaatagg tatagaggca
     6421 attaaatctt tatttaactt agatgcatta agtgttccat caatattacc attagtagta
     6481 tcagttattt caatcctgat aaaagagtat caatatagaa taacaataaa agttgctaaa
     6541 aagataaatt caccagcctt aaaagctgat gcttggcatc ataggtcaga tgctttatct
     6601 tcagtagctg cttttatagg tataggtggt tctattttag ggtttaagcc actagaccca
     6661 attgcgtcaa ttgtagtggc tatatttgta gctaaagtag gaattagtat acttataagt
     6721 tctgttaatg aattgatgga tgtttcagtt gacgaagaag agataaaaga attaaaattt
     6781 attgtagcag atacagaagg tgttaaaaat cttggtgata taaagactag aaaacatgga
     6841 gctatggcat atgtagattt gactatatgt gtggatgaaa atttgacagt taagcaaggt
     6901 catgatattg ctaccaagct tgaaaaacat ataataaaac atatggaatt tgtaaaaggc
     6961 ataacagttc acgttgaacc atgtactaat tgccaaggaa ataagtgtaa taattaatta
     7021 aaacttaaaa aaaagtgtaa aaagttatat aattgtttta tgattataaa ctctaggggt
     7081 ggttaaatga gcaagaaaag aaaactaaag aaaaaagctg caatattaat aggagtagtt
     7141 gttttcttta taatatactt tatatccttt agaataggaa taaatatgga aaaggataaa
     7201 agtgaagtgc caaagaaggt agaaaaagaa gaagaagata ataggtcttt aaggactcaa
     7261 ataaaagaaa agaaaaaggt ttatatatca gacaaaaata tagaaaacat aaaaattgaa
     7321 gaagatatgt gggatgaaat aaagttattt ttctcagatt ttaagaaagt aagaaatact
     7381 acttcttctt acgaagcagt ttatgaaggt tattctgatg atggagttaa attttctact
     7441 gaccttaact tttttagagt atatactgta aataaagaag aatattataa agtaccagtt
     7501 agttctaagg atgcttttaa gaaactttta gatgagagta tatatacttc atttgacctt
     7561 ttaagtcatt ataaagattg gaaagaggtt actataactc atggaaaaga aattaaaaaa
     7621 gttcatagat ggaaatttga tgatttagca cataagatga tttcaaagag ggttgtagga
     7681 aaagttcaac ctgagaaaag taaggaaaga agtgactata attttactat aaatataaaa
     7741 ggtgataatt ttgatgtaaa agttgataca atgggaaaag attatgtaaa agttacttct
     7801 aagaaaaatg tatcttacta tgaggttcat acaagtttat ttgattacct taaaaatgag
     7861 atatttgaaa ttggaaagaa ataagacttt aagaaggcta tcaaattaga tatttgatag
     7921 cctaaattat ttttatatat tatttttcaa ccaacaagtc tgcaacctta taaattggac
     7981 cagcaccagc agttattact aaatcattat ccttaacatt ttcacgtaag tattttacta
     8041 tatcttcaaa ttcttttata tagatggcat ctacattgtt ttgatataat ttctcaacca
     8101 agtccttaga atgtatatct ccagggtctt tttccctagc agcatatata tctgttatga
     8161 taactttatc agcagcatag aatgcctctg aaaattcacc taaaagagat tttgttctag
     8221 tataagtatg tggctggaag atacaccata aagttgattt ttttaatttt ttggcagcag
     8281 ctaaagttgc ttttaactca gttggatgat gtgcataatc atctactact aaagcattct
     8341 tataataccc tttagtttca aatcttcttc cgacaccttt ataaattttt atattttttc
     8401 ttatagtctc taagtcaata ccagaaacaa gagctgccat tatagcagaa gaagcattgt
     8461 atatgttatg aagaccatat acagaaagtt caaattctcc taaatctctt ccttcatatt
     8521 ctattctaaa tattccatga ccactattgt caaagtgaat atcttttatt acagcatcat
     8581 tgtcagaatc tctaccgtat tttataatag tagctttaac atcatacaat atgtcatcaa
     8641 tattttcatc atcgccattt attatgaaat agccttctgg gggtaataat tttccaaatt
     8701 tgttaaaaga agccttgatt tcatctattc cagagaaata atcaagatgg tcttcttcaa
     8761 catttaaaac tatagatatc tttgggttaa aattaaggaa actatctaca tattcacatg
     8821 cttcagttat aaagtggttg gaattaccta tcttaacatt accacctatc atacttaagt
     8881 taccaccaac tagtatagtt gggtctaagt ctgcatattc aaagattgta gataacatag
     8941 atgttgtaga agtttttcca tgtgttccag aaactgctat agaattttta tattccctca
     9001 tgatttggcc taaaaaagtc gctctgttca ttaccaattt atttttttct ttagcagcca
     9061 caagttcttc attgtctgga tgaactgctg ctgtataaac aaccatattt acatcatctg
     9121 atatattatc ttttttctga ccaatataaa tagttgcccc ttggtcccta agtttgtcta
     9181 ataggtatga ttcgtttgaa tccgaacctg aaacttgata acctttattg atacatattt
     9241 cagctaatgc actcatgctg ataccgccaa ttcctataaa gtgtatattc ataataaaac
     9301 cacctttctt tttaattaag tcctacaata tgaactattt atgtggtata ataatataat
     9361 gttcaatatt ttataattaa tatgcatata atattatatg tattttattt ttgaatataa
     9421 taaaacataa gtaatattat accacaataa gaattgttat gcattagtta gtttaacgtt
     9481 atataaaggt acttgtagga ggaaataata atgaaattta aaagaacaga gagaattgga
     9541 gcaatagtta aaatactgtc tgataatcca aataaaatat atacattaag ttattttaca
     9601 aatcaattta atgcagcaaa atcaacaata agtgaagatt tattagttgt aaaaaatgta
     9661 tttgagaagc tacatttagg taaagttata actatctcag gagcagcagg aggagtaaaa
     9721 tatataccta aaacttctat agcagaaaat gcagagttct taatggaatt atgtgagaga
     9781 atatgtgaca agtctagaat actatctggt ggttttttat acttgattga cctaatttat
     9841 gaccctacaa tagctgctaa gataggaaag atattcgcat caaatattga gtatgtagat
     9901 gcagattatg ttgtgacaat ggaaacaaaa ggtataccta tggcacttat gactgcaaaa
     9961 gctatgaatt tacctctagt tataataaga aaagatataa aggtatcaga aggtcctaca
    10021 cttagcatga catatgtgag tggaaacagc tctaaagttg aaagtatgag tttgccaaga
    10081 aaagctctta aaccagatag taaagttata attatagatg atttcatgag gggtggaggt
    10141 actataaaag gaatggtaga ccttatgaat gaatttggtg cagaggttat aggtactgga
    10201 gtattcatat ctacaacgaa tccttcagaa aaaatggtaa aagattatat ttcattgatt
    10261 caattggatg ttaatggaga taaaatagta gtagaaccaa acttaaagac ttttaaagat
    10321 gaatatagaa atgaagattt agacgaagaa gaagattcag aatttgaatt tgagatagat
    10381 gaagattaat aaaaaaaata tttagaaaaa tatgtaaaaa taaaaagagg atatccctag
    10441 ttgttcatag aattatttaa aaaacagttt atattaattt attccagcga taataatttt
    10501 aatataaatt aatttctgtc aaaatcaaag ggggatattt tggtatgaag ataactgacg
    10561 taagagtaag aaaattaaca gaagaaggta agatgaaatg tatagtttca ataactttcg
    10621 ataatttgtt tgtagtgcat gatataaaag ttatagaagg ccataatggg ctatttatag
    10681 caatgcctag cagaaaagta ggcgaaggaa atttcagaga tatagctcat ccaataaatg
    10741 cagaaatgag acaagtttta gaagatgcag ttttacaagc atatcatgaa gcattagttc
    10801 aatgggaagt agctgctgaa taataaatat ataaaatgga gagagcccga acatttaggc
    10861 tctttttaaa tgtagaattt ctattaaaat atgtactttt tagtaaaaaa gtgctatatt
    10921 attagtgata atgtatatgt tgtatacaaa ttgttagata aatgtattat aatgtatatt
    10981 gattaagaga ataatacgaa taaaattaaa ttagattgaa tggagtgtat caatgaactt
    11041 taaagctata atccttgctg ctggcaaagg aacaagaatg aagtctaaat acccaaaagt
    11101 tatacataaa gtatgtggaa aagaaatggt taaccatatt atagatgttt caaaaaaatc
    11161 aggggtaaaa gatactgtcg tgattttagg acatgaagct gaggtagtta aagaaaagct
    11221 tgcagaagaa attataatag ccatgcaaac agaacaactt gggacaggac atgctgtaaa
    11281 gatggctaaa gaatatatta atgatgaaga tacaatagtt gtactttgtg gagatactcc
    11341 acttataaaa gaagagacac tgaagagatt atttgaatat catatagaaa ataaatatca
    11401 tgctacagtt cttacaacaa gagttggaaa tccaacaggt tatggtagaa taataagaga
    11461 taagaagggt gaccttctta aaatagtgga acaaaaagat gcaaattcag aagaaaagat
    11521 gataagtgag ataaactctg gaatttattg ttttaatgga aagagtctta gagaagcact
    11581 agatttgcta aataataaca attcacaagg tgaatattat ttaactgata ctgctaaaat
    11641 aatgagagat aaaggattaa aagttggtgc atttgcaggt tcaactattg aagaacttat
    11701 gggcgttaat tcaagagtag aattatcaaa ggcagaagaa attatgagac gaagaataaa
    11761 tgaatctcat atggtaaatg gagtaactat aatagacact aactctacct atatagaatc
    11821 tgatgttatg ataggcaatg atactataat atatccaggt gttatgttac aagggaaaac
    11881 aaggataggt tcagattgta taattggaat gaactcatct ataacaaatt cagaaatagg
    11941 tgatggtaca gaaataaaaa attcaactat aattgatagt aaggttggag aaaattctaa
    12001 tgttgggcca tatgcatact taagaccaaa gagtgactta ggaaataatg taaaaatagg
    12061 cgattttgta gaagtaaaaa atgctattat agaagatgga tcaaaagcat ctcacctttc
    12121 ttatatagga gatgcacatg tcggtaaaaa tgtaaatatt ggatgtggag tagtatttgt
    12181 aaactatgat ggtaaaaata aatttaaatc agtagtaaaa gacaatgcgt ttataggttc
    12241 aaattctaat ttagttgcac cagtagttgt agaagaaaaa ggatatatag caacaggttc
    12301 aacaataaca catgatgtcc cagatggtgc tttggcaata gcaagagaaa gacaagttat
    12361 caaagaagga tgggtagaaa aaaagaatca aaaagacgac caaagtaagt aattatgtag
    12421 tttattattt tggctaaaaa atttagctcg aaattattat aaattatatt cattagtatt
    12481 ttaaaatttt caggagggtt acataaatga atactagcgg aagcgagatt aaaattattg
    12541 caggtaattc atcgaaagag ttagcacaaa aaatagctga ctatataggt gtatcagtac
    12601 tagattgtga agtaggtaca tttagtgatg gtgaaatatg tgtaaacatg aatgagacag
    12661 taagaggttg tgatgtgttt gtagtgcaat ctactaacag cccagtaaat gataacttaa
    12721 tggagttatt aatcttaatt gatgcgttaa agagagcatc agcaggtaga ataacagcag
    12781 tcattcctta ctatggatat gcaagacaag acagaaaggc taaggcaaga gatccaatca
    12841 ctgctaaatt agttgcaaat ttaataactg ctgctggagc agacagagta ttaacaatgg
    12901 atttacatgc agctcaaata caaggatatt ttgatatacc attagaccac ttactaggtg
    12961 gaacaatatt agcaaattat tttaatgaaa agaaaataga agatttggta gtagtatctc
    13021 ctgacttagg aagtgttact agatcaagaa aatttgctaa tactttaaat ggagaagttc
    13081 caatagctat aatagataaa agaagaccga aagctaatgt atgcgaagtt atgaacttaa
    13141 taggtgatgt taaaggtaaa aatgttatac tattagacga tatgatagat acagcaggaa
    13201 caatagttaa tgctgcaaat gcacttaaag agtttggagc aaaagatgtt tatgcttgtt
    13261 gtactcatgg tgtgttatca ggtccagcta ttgaaagaat tgctaattca gaaataagtg
    13321 agttaatagt tcttgatact atacaacttc ctgaagagaa aagaatagat aagataaaga
    13381 taaaaacagt agctccttta tttggagatg ctataagaat gatattctct aatgaatcag
    13441 taagtaaatt attctaattt attgtattta tgagagttgt ttaaaaggat ataaaccttt
    13501 tgagcaactc ttttttgatt ttatacttat ggaatgttat ttttttcttg ttagaaaggt
    13561 atatattatt gtaaaaagag attatagttt aaaattatgt atatttaagt aaaataaact
    13621 taataaaggg catattattc aaaatattat gaatataaaa aatattacaa aaaagtattg
    13681 aaaaaattca gattatgtag tattatgtaa atataaataa agaaagaggt gtttccaatg
    13741 ggaaggttag aagatgaacc cctaataatt aaatgattca gtcacaatct aaagtgaata
    13801 taaaaatcta ttgaaagttt atttataaga attgaaaata gtatctattc tatctttagt
    13861 gtttgtgaga gggttctgga ttatataaga ttactgtaaa gagtaatttt gcacaataag
    13921 catagctgta aatgaagagt ttcgtttata taatgattgt ggctgaatca taactataat
    13981 agatagaatg tttataatag atgattttat aaaatactat attgtaatag cttttagctt
    14041 tcaaataaat attagcaaag gatggaaatg acaatgataa aactaaatga taatttataa
    14101 atatctcctt acgatatata ataattgtag ggagatgttt cttttttttg tagagtataa
    14161 tttttctaga aattctgttt caatatgtaa aaagttggta tatataaata gtataaacag
    14221 tgttgctaaa atatggtaga tattatataa tataagtgga tatgtatata cattatgagt
    14281 tatataattt tgaatttaat aaaatagaaa cgttgattta tgttctgtaa tgtgggcacc
    14341 ttagagcata ttgagttagt ggtgcaaccg gctatgaaat tgtatttatt tatagatact
    14401 atcattttca tagctttttt tatttaaaaa atggttatgt gttagaaata aatttggtta
    14461 gtacgtttaa ataattttac ttagattttt aaccagatat attataagta agtattaatt
    14521 aaaaaattta agggggaata aaaatgaagt taaaaaagaa taaaaaaggt ttcactttag
    14581 tggaattatt ggtagtaatt gcaattatag gtatattagc agtagtggca gttccagctt
    14641 tatttagtaa tataaacaag gctaaggtag caagtgttga gtctgattat agttcagtta
    14701 agagtgcagc attatcttat tattcagata ctaataagat accagttaca ccagatggtc
    14761 aaactggttt aagtgtttta gagacttata tggagtctct gcctgataaa gctgatatag
    14821 gtggaaaata taaattgatt aaagttggta ataaattggt attacagata ggtacaaatg
    14881 atgaaggagt tacattaaca gaagcacaat cagcaaaatt attgagtgat ataggtgaaa
    14941 ataaaatata tacaagtgtt acagcagata atttgggaaa tccattaaca agtaatacaa
    15001 aagtagataa taaagttcta tatatagtac ttatagataa tactgtgatg gactcaacaa
    15061 aatagttaaa actatatttt tataaaaata ttgtaaattt aatgaaaaat ataatataaa
    15121 agacaggaag aaaatcttcc tgttttttta tactattttc tttaatgttc aattatgaat
    15181 gatgatataa ttgagatagt aaaggagtga aacctgtggc taaaaaagtt agaataggag
    15241 acaaactggt cgaaaaagga tatataacag aagaccagct taaatgggca ctatcagaac
    15301 aaaaaaacag tggaaaaaga ctaggagaat ttctagtaca agaagggctt atagatagta
    15361 acttactaat aagtgtatta aaagaactct tggatataga aagtatattt ttagaaggga
    15421 cagaaataga cactttagct acaaagatgg ttccagaaaa tatatgcaaa agatatactg
    15481 tatttccatt taaaattgat ggaaataaaa tatgtctagc catgtcagac ccacaagaca
    15541 gagaagcagt tcaagatgta aggcgtatgt ctggtaaaga cgtagagata tttatatcaa
    15601 gtacagaaga tataaacaaa gcaataggtc atgcttatgc acattcagaa ataaataagg
    15661 caatgactga gtacaacaaa aatagaactg gtggagtaag agaaacagta atacttgaag
    15721 aagatgtaaa tgctgctcca atagtacgat tagtaaataa catactagaa aatgctgtta
    15781 gaatggaagc aagtgatata catatagaac aaagtgaaaa ttacatgcgt gttaggttta
    15841 ggatagatgg aatgttaaga gaatatatga ggatgaactc agcaccatat aaggcagtaa
    15901 tcagccgtat caagataatg tcagatataa atatatctga aaagagaata ccacaggatg
    15961 gtagaatcta tttaaaagta gataataaac ccatagattt tagggtttct acaatgccaa
    16021 ctaacaggga tgaaaagata gctatgaggg ttcttgataa gagtaatttt atggttagta
    16081 aagaagtact aggaatagac gaacatggct ctaagatata tgatgaattg ataaacactc
    16141 cttatggtct tatattggtt gttggtccta ctggaagtgg gaaaaccact acattgtatt
    16201 caatgttaaa tcaactaaat acagaaaata gaaatctatt aacaatagaa gaccctatag
    16261 agtatgaatt acctggtgta aatcaatctc aaataaatga aaaggcaggg cttactttcg
    16321 ccagcggact tagagctttt atgcgtcaag acccagatat aataatggtt ggagagataa
    16381 gagatactga gacagcagaa atagcaatta gagcttctct tacaggacat ttagttttaa
    16441 gtacattaca tgcaaataca gcagttggag caatttcacg tcttttagat atggatgtgg
    16501 aaagtttttt aataacttca tctgttttag gtgtaatatc tcaaagactt acacgaaaaa
    16561 tatgtgagca ttgtaaggtt tcatatgaag cagatattgg agaaaaaaag gctcttggta
    16621 ttgatgtaaa tgaatcagta actatttata gaggaaaagg ttgtgaaagg tgtaataata
    16681 ctggatataa aggacgtcta ggtatatttg aaatgcttga aattactcca gaaataaaag
    16741 agttaataga ttcatctgca aatcaaagag aaattttaaa gatggctaga aaacaaggca
    16801 tggttagctt aaaagaggat atagttaaaa aggttttaaa tggaaagaca acagtagaag
    16861 aaatgattag aataattttg atgactgatt aaaggatgat attatgaaaa gttttaaata
    16921 cacagtaata aaagaggacg gaaaaaagca aactgatgtg attgaagcca acgattttaa
    16981 tgaagctaga aatcttctta gaaagagaaa tcttagagtt attgaaatca aagaaagcaa
    17041 aaataaaaat ataagattat ctagtagaaa gagaaaaaaa gacttgggag ctgaccagat
    17101 aagccacttt tgtagacagt ttgctattat agtatcttct ggtataaata gtatttcagg
    17161 attagaaaca ttagctagaa ggtcaactaa cattacatta agagaagaaa ttaatcgtat
    17221 agtggcagaa ataaaagtag gttctactat tgcagattca atgctttcat caaaatcaaa
    17281 gtttccaaag ttacttggtg ctatggtagc tacaggtgaa gctacaggta aattagatga
    17341 agtattaaaa agtatggctt cattttatgc aagtgaacac agggttaaac aaaagcttag
    17401 aaatgctgca acatatccat taatagtttt gatttcttca tttataatga tttttatatt
    17461 tacaacattt atggtgccta agatgattaa ttcaattaca acagtaggag caactcttcc
    17521 acttataaca agaattgtta tggggtttgg tatgtttatg aagaagtttt ggataatagt
    17581 acttttagtt attatattat ttatctatca gtttaaaaaa tatttaaaaa cgtcagttgg
    17641 aagggctcat aaagatagag ttataaacaa gatacctgtt ttaggaaaag gaataaattg
    17701 tatggtagct acacgatttt caagagcatt gtatctattt gtatcaacag gatacccatt
    17761 agttcaaggg cttgattata ttatagatag tgtaaataac accatggcag agaagatttt
    17821 agcatcagct aaagatggaa taacaagagg agaaggctta gcagaaaatt tagagagata
    17881 tacgtatttt gactctgtgt tggtacagat gatagctata ggggagcaga caggggaatt
    17941 agagaatata agtagacaga tggcagaatt ttatgagtat gaatccgaga tttatttaaa
    18001 taggatggcc tcaatgatag agcctgtttt aataattgca gttggtatta tagttgcgat
    18061 actggttgta tcaatattta tgccaatgct tagtatatat gatgccatgt aggagggaaa
    18121 ggacaaatga ataaggttta tgtatcatat tatggcgatt ctatttccat agttgagggc
    18181 agatataaac acgataagga taagttttat gtaaaaaatt ttaatgtgtt ttcaatagag
    18241 gatattgcac aagatttttc taaggaaaag tatgctctat tgagatacgc attagaaaat
    18301 gttaagttaa aatcaagaca tgtagttttt tgtttaaata caagagatgt aattttaaaa
    18361 ccccaaaaac ttcctaatat ggacaaaaag gatttagatg gatttatgaa gctagagatg
    18421 gacgaaatga tggctttgga gtcagatgaa tatgtatttt cgtatgaagt atctagtgaa
    18481 acacatgata attcagatgg cgatgaatca agcttaaatc ttatcattgg agctatacaa
    18541 aaggaagagg taaatgcaat tgtagagata atgcatgaat ttgatttagt attagataga
    18601 atagatacat tatcaacagc ttacttaaga atgcttaaaa atttagacta caatgatata
    18661 atggtaacca atataagtga caatagtaca attataaata tatataagaa agatacttta
    18721 tttattagtg ataatatacc tataaagata actgctaaag atatggacat gcaattatta
    18781 aatgtagtag aagaatcaaa aggtcttatg aattactatt cgtcaagaaa ctttggtaag
    18841 gttacggata ctatattaat attaggaaaa agatattcca ataaattaat ttatgaaact
    18901 tttagctctg cattttcaag ctatgtttct caaggcctta ctgagataat ggatatatct
    18961 gatatgataa ctggagatat agatgaagat gatataaatt caattgtaga gcttttagga
    19021 agtatgatag aacctaaagg gaaaagagat tttgaaaata taaacttttt accaatggat
    19081 atacttagaa aacagataaa agctaaacaa atgaaaaaca tactgaaaat tgctccaata
    19141 gttattttga ttcttgctat accttattta tgtttaattt tttcaaataa tttagcaaat
    19201 aaggaacttg aaaatgttaa tggtgaaata agaaatattg aatctgcata ttatcagatt
    19261 ggaaatatag aaaaaatgat acagagtaaa agcgaagaaa taaaggtata tgatatgtta
    19321 attgggaaaa aggtaaagtg ggaaaattta ttggatgata tagataaaaa tactccaagt
    19381 aaagttgaac taacaagttt atcaacaact tatcaaaatc ttggaactaa taataaagct
    19441 gataattcaa aaagtaataa gactacaaca gataaatcaa gtaacaatga aacaaaatca
    19501 agtacgaaaa gtgatagtaa tggagataaa acagataatg atttaaatga agataagact
    19561 acaaatgtgt cagaagcatt aactggaaat agtgagacta atagtaataa cacggataaa
    19621 gatgataaaa ctccattgta tgataagata ccaaatgtta taaatataga aggaaatgct
    19681 aaagattcct cttatgtagg tcaattttta tataaattaa agggattata ctatttcaag
    19741 gatgttaaat tacatagcat aaaaagtaat gcagaagatt ctaactatac atttagtatg
    19801 acattatacc tgaaagaagg tgtattaact aatgaataaa gatatattta aaaaagatat
    19861 aaaagatata tttaaaatgg aagttagtct aaaagggatt cttataaagg ttataatttt
    19921 attagctatt ttatcatatg gttattttgg actttaccct caatatcaga aatatattgc
    19981 tacaaaaaat tctgtaaaac aggctactga aaaattaaat atatataaag agaaaactgc
    20041 acttatgcca aaatatgaaa aacaacttga agaagttgaa agtgaattaa aagaaaaaag
    20101 caaaaatctt ttatatgata tgcaagatgg tcttttttta ataggtttag ataaaaaaat
    20161 gaaggcttat aatattgact taactagtta tgacgttgga aatgttaaag aatacgaaca
    20221 tttttatggt atatcaacaa caataagtgt tagaggtgat tatagaaatg tcaaaaaatt
    20281 aatggcttat ttagaagaac aaaaaaatgt tactcaaata ctagattata caatgacttc
    20341 atatatagaa ccagaaaaag aagaggaaga aacaacaaac aataaggatt taattaaaaa
    20401 tgtgaacata aataaagata caaaaatata ctgggtggaa ggcgaaaata agtggcattt
    20461 atataaggat tgtccattga taaaagaagg agagattcta aaaaatgaca aacttttaga
    20521 taggtctact aattatctat gtaatacttg tgtacttaga gctgcaacag aaaaagaagc
    20581 aaaaaaagaa gtcgtagctc caaaggcaga aggtgtagta gatgctacat ttaaatttat
    20641 aatgtacact aaagagaata ctacaatgat acttgataca gataatccat cttcatggag
    20701 accaggaaaa tataatccat ttaagtctag tgctaaataa agctaagtaa gctttaaaga
    20761 taggtgatga gtttgtacaa aaataatgaa aaaggtctta ctttattaga agtaataata
    20821 gctgttttta tactgacaat agttttaagt atttcttata aagtgttcaa tggtataaca
    20881 tcagcagtaa aaaagcaaca gattattaca gatgctcaag taaatattaa tctaattaat
    20941 aagtatctaa atagagattt ggaaaactgt aaagagctaa ctaaaactgg ttcaggtaat
    21001 aattatgaat acaatataga gatgccagat aatgttgtaa aatatgaagt ttctatagaa
    21061 actaaaaaaa atacagaggt atattctgta acgagaatcc aaaacaatac aattgataca
    21121 gaaaatgaag ttagagaaga gataatctat aatcaaccac tagttcaaaa caataaagaa
    21181 atgaaggaaa caccatttaa aatagaaaag caaactggta aatctattta tactgtaagt
    21241 atatactata atgaatcagt gcaagaaagt cataaaaata gcaatttaaa taacaaaact
    21301 tatacatttg atgttatgtc aagaataggg taataagaat ttattaaaaa ataagtaggt
    21361 tgataaaata tgagtaaaaa gaatagtgaa agaggatttt ccttaattga agtgttagta
    21421 gctatggcta ttatgggaat tgtactattt gcatttttta atattatcaa tactaataac
    21481 aaagcaaaca ctaaaaatga cacagatata acttccttaa actatgtcca aagtgaaata
    21541 gaaaatctaa gagaaaagat aaaaagtgga gaatttgatt ttgatagttt agataaacta
    21601 gaagatggaa ctgtcgtata tgaaaaatta atagataaat caaaaaaagt tgtatatgat
    21661 aaagttctta gtgaaggtga cgtgagctta tatgatactc catatgaaaa gattacaaca
    21721 ataaaagatg aagatggtaa tttaatcgac aaaggaaata ttacaaataa gataaagact
    21781 attgtagaag ataaaagtgg tcagatatat aaaatagctg taactgggaa aagcatgaat
    21841 gactattcaa gtaaaaaaga ggttaaaatt gtaactgaaa tatttaaaga taaataattt
    21901 tacttggggg tgagattttg aggaagtgga ataaatttaa aagtgaaaga ggggcagctt
    21961 tagttttagt tttgattgta gtagcattgc tttctattgt tggattgata ttttcaaatc
    22021 agatagctaa tagaataaaa tctacaaaga ctactaatga aggtatacaa gcaaagtatt
    22081 tagcagagac atgtgtggaa aatagtattg ataaagcata tgaaaaattg tatgatgagc
    22141 ttgagaaaat ggataatgag tttaaaagtg aaaatcaaga aaaatccata tctaggtcaa
    22201 aattaagaaa tatatcagat gaagatttta ataatcaaga tgagaaaaat attgaagcag
    22261 aaagacttgg atatatgaat aatataaatt tctatttaaa taaagcttct tcagacttgg
    22321 aaaaagctag tatggaattg aaaaagctat atgatttaga tatgttggac tatagagaca
    22381 ttgaatatgt agatgcaaat attatttcac atagagattc tatcttagaa atttgcaaga
    22441 attatacttc tggagatatt agtaaaataa atgaatatat tctgaaagaa gatatagact
    22501 caactacatt gattgaggca aagttagtga ataatgatat tcttttgaaa atgtttttag
    22561 aagaaaataa aattgaaaat gaacatttaa attcagcctt ttcacatact tataaggcat
    22621 tagataacat ttctttggcg atgcaaaata tgattgagta tagacatacg tttcatatag
    22681 atgagccaaa agtagaagta tctaatggga ttccagattc tcaacaatat tatgaactaa
    22741 tacaaaatcc tataataaat agtatggagt atatctggaa tagtaaatgg gatacattag
    22801 aaaacctatt ggaaatttta ccaaatcaaa ctcaaggatt taattcttta agagttcatt
    22861 tgagaaataa tgtaagaaaa tttgagaagt taagcgataa tatatctagt ggtaaaaaaa
    22921 atacagcaaa aaatttttta aagtataaag agttattgta tgaaatatca gaccaatgta
    22981 atcaactaaa aagtatgtct tatgagaaaa tacctgttaa atatgataat atggctttga
    23041 taacaacttt tgactatata caaaatgagc ttttagcaga gataaaatgc agattaaaag
    23101 aattaaaacc acaagaaatt gataaaacag aaggtattac tataaaaata cctttttata
    23161 aggctgacta cgacatgaca aaagaaggat ggcctaaatt aaaagaaaat ggttcagggg
    23221 cggagttaag cttaatggta actggtgata aagatggaat aaaagaagta gaagttacag
    23281 atggtaaaaa aaatattatt ggtttaggtg ttgaagaaaa ttcaaattca aagtataagg
    23341 ttgacgccat tgtaaacttt aatctaaata tagatacaaa tgtagttgga aattatgata
    23401 taaaagataa aattttgatt aatcatgata taagttcata caaaaaagta aactaaatat
    23461 aagaagcaac ttagtataaa taaataataa aataatttta ttatttacta ctaaaaaaat
    23521 aagtaaacta atagaagata tggagagtaa atattatgta tatttatgat ttattagagc
    23581 aaggtataag actaaacgca tcagatatac acataacagt tggaacaaat cctgtagcta
    23641 gagtaaaagg tggatttgta aaactaagtg aacaaatatt gacatcagaa gtaacaatgc
    23701 aaatggcaaa agacatagcg ggggaatcta tttttaaagt aatagaagaa catggagaag
    23761 ctgatttttc agcttctcta aaaacaggag aaagatttag ggtaaatgca tataggcaaa
    23821 aaggaaatta cgcaatagca ataaggacta taacagcaga aattccaaca tttgaaaaat
    23881 taggccttcc agagagtata aaatctttta ctgaaaagca taaaggatta gttttagtta
    23941 caggtccaac aggaagtggt aaaagtacaa cacttgcaag tatgataaat ataataaatg
    24001 aaaaacaaca aaaacatata attacacttg aagacccgat agaatatgta catcatcata
    24061 aacaaagttt agtaaatcaa agagaagtag gaactgatac agaaagtttt cattcagccc
    24121 ttagagctat tttgcgtcag gacccagatg taatactaat aggagagatg agagaccctg
    24181 aaactgtttc aatagcatta acagcagcag aaacaggtca tttagtattt tcaacactac
    24241 atacagtagg ggcagctaag acaattgata gaatagtaga catgtttcaa ccatcccaac
    24301 agcaacaaat aaaaacacag ttatctacag tttgtgaagg tgtggtatca cagcagttac
    24361 ttcctacagc agatgggaaa ggcagaatag cagcaattga attgatgttt gctactccag
    24421 caataaaaaa cttgataagg gaaggcaaaa catatcaaat tccaaacatg atacaaactg
    24481 gagttaaagt aggtatgaaa actatggacc aagatttaat ggaactttat aaaaatggaa
    24541 agattacaaa agatatggca cttagtaggt gtactgacca agagtttatg acaagaatga
    24601 taggtggagt aaactacaat ggatattata atcgttaatc tatatgtatt tatagtgggt
    24661 atagtgtttg gaagtttctt taacgtctgt atttttagga ttccagaagg tatatctatt
    24721 tctagacctc cttcccattg tatgagttgt aatactagac taaagccaat agacttaata
    24781 ccagtgctaa gctatttgat gagtggcaga aagtgtagat attgttatga gaaaatttct
    24841 agtagatatg ctatagtgga attaattaca ggaattttat ttttaacagt atttatgtta
    24901 tatggaatat ctacatcaac tatttattat ttggttcttg tgtctttact gattataatt
    24961 acatttatag atattgacca ctttataata ccagataaaa tattgatttt tggagctata
    25021 ttttctatga tttttaacct tttatttaaa gaaattccag ttaaggattc aattctaggt
    25081 gtttttattt gtggaggaag tgtctggatt atagttcttt tgattgaatt tgtaataaaa
    25141 aaagagtgta tgggtggagg agatatcaaa ctatttgcaa tgctaggcct ttacatgggt
    25201 gttaaaaatg gtcttttgac agccttacta agcgtttatg taggagcagt ctatggaatc
    25261 tgtgtaataa ttataagtag aataaaagga aaggaatata attctgttat accatatgga
    25321 ccttttatat ctattggagc attaataaca atcttatgtg gaaagcaatt acttgaactt
    25381 tatttttcta tggttatata gatgatagtc aagttaaata aagttttaat ttaaaaaaat
    25441 gaattaaagt atttaataag gacaatatta ttattaaccc tattactaaa gtgctacaaa
    25501 ctatggagta tataataata atttccttat tttttgcaca tttgataaat aaatctctat
    25561 gtgttttttg tgatagagaa aagaaaaatt tatataatac gttttttatt tacataataa
    25621 ctgtagtttt atctgtatta atatatctaa aatttgggat tagtgtagaa tgtgtacaat
    25681 atatggtttt aattccattt ttaataatta tatccattgt agattactac acaatatatg
    25741 tatatgatat aacaatagtt agtggtataa tagttcaagg tataattttt ttggcctttt
    25801 tcaatatagc tcaatttgat tatataaatc atatatttgg actattgata ggctttatta
    25861 taccttatat tattgctaaa ctaacaaaag gattaggcta tggagatgta ggtctctata
    25921 gcttatgttg ctttgcattg ggaaataatt atgggtttta tgtgatagtt ttatcattta
    25981 ttttagcgtt tttgtttttt attattttat acataactaa aatatttaaa gatgtagata
    26041 taagaaagca aattcctttt acacctttta tttccttgtc aacaatttta ataatattta
    26101 cagagtataa tatatttaat ttttattata atttgttata caaatttata taacaggagg
    26161 taagaaatgt acgtagtagt agggttaggt aatccaggaa agaagtatga gaagactaga
    26221 cataacgttg gatttgatgt tatagatatt cttgctaaag aatacaatat tagtgttact
    26281 aaaataaagc ataaagcttt gattggtgaa gggagagtag gtactgaaaa agtattatta
    26341 gttaaaccac aaacttatat gaatttaagt ggagaaacat taatagatat atataaatat
    26401 tataaggttg atttaagtaa tatagtagta gtatatgatg atatagattt agaagttgga
    26461 aagatacgta taagaaaaaa aggaagtgga ggaactcata atggcatgaa atctataact
    26521 aagtgcctag gttcaaatga ctttccaaga gtgagagtag gtgtatctaa accagaagct
    26581 ggtcaagact tagcagactt tgtactttct agatttagaa aagaagaatc tgataacata
    26641 aatgaagcat tagaaaaagc ggcagatgct attgactcaa taataagaga aaatatagat
    26701 atgtctatga acaaatataa tggatagtga gagggagatt taaatgaatg atgttttttt
    26761 atatcctttg caaaattcaa aagagtataa agatataata aactgtataa aaaatacaaa
    26821 aggctcatta ttggttaatg gacttttacc agtacaaaaa cctcatatat cgtattctat
    26881 atttaatgat ttatcacgac aaatgatttt tataacgagt agtgatttag aagcaaagaa
    26941 agtatacgaa gatttaagtt tttatatgga agataaagtt gaatatttag gttttcaaga
    27001 tatttatttt tatcacttgg atgcaaagga cagaaacgaa gaagcaaaga aattaaaagt
    27061 attgttaaaa ctagcaaata aagaaaaaat cattttagta acttctattg aggcagtttt
    27121 aagaaaatac ataccaaaac aagttttgct cgatagtgta tctcattata aggtgggaga
    27181 tagcttagac ttagaaaagt taactgagaa attagtttca ttgggatatg aaagagtttc
    27241 aaaaatagag gggtttggtc aatttagtat aagaggtgga attattgatg tattttcact
    27301 tgaatataca aatccaattc gtatggaatt atttgatgat gagatagatt ccatacgaac
    27361 ttttgacgta tattcacaaa aatcaattga taaattgcaa caattctcta taactccatc
    27421 tagagaattt atttatcccg aaaaaactac agatgcatta gttaaattaa aaaaagaaac
    27481 aactaaaaat actgatgaag gtacatttca aaatatagat tatatatcaa gtaagactta
    27541 ttttgaaggt gcagagaact atatagatta tatttatcca gaagaaaaca aaagtatatt
    27601 tacttattta gcagatgatg caatagtatt tattaatgat ataacgagac ttaaagaaag
    27661 atgcgaaaat tatataaatg agtttagaga aaattataaa ttaaatcttg aaagaggtct
    27721 ggctattaaa aaccaaggta aattgttata tcactataca gatttagagt acttagtaaa
    27781 agacaagtct gtggtattaa actcgctatt gccaaagtct ataaataact tttctataaa
    27841 atctataata aattttgaaa gtagagaagt tcctacattt aatggaaagg tagatttgct
    27901 tgtagaagaa ttaaatcggc tgaaatataa tggatacaaa ataattttag caacaaatac
    27961 cttagaaaga gcaaataaat taggaaaaga tttattggac aaaggcttag aaacgaccat
    28021 ttctaaagat agagatattg aaattaaatc ttcacaagtt ataattgtac ctgcacacat
    28081 aaacagtggt tttcagtata aatctataaa gtttgttgtt ataacagata acgaaatgat
    28141 aggtgtttat aaaagagcgt ctaagactag caataaaaaa gttaaaaaag gaaagaaaat
    28201 agaatccttt ttagatttaa gtgttggaga ctatgtagtt catgaaaata gtggtgtagg
    28261 tagatacaca gggattgaac aaattacagt caatgctatt aaaaaagatt atatgaaaat
    28321 agtatatcaa ggtggagata atctatatgt gccaatagac cagatggaca aagtacaaaa
    28381 atatattggt gcagaagtag aaaaagttaa gttaaataag cttggaacaa atgagtggac
    28441 gaaagcaaaa gcaaaagtta aaaaagaaat tgaagatatg acaaaagact tgattgagct
    28501 ttatgcaaaa cgtgaaaaaa tacaagggta taagttttca aaagatacac cttggcaggc
    28561 agagtttgaa tcgttgtttc catatcaaga aacagaagac caattaaagg ctatagaaga
    28621 aactaaaaaa gatatggaat caagtaaggt aatggatagg ctagtatgtg gagatgttgg
    28681 ttatggaaaa acagaggtag ctattagaag tatttttaaa gcttgtatgg accaaaaaca
    28741 agttgctgta ttggttccaa caacaatatt ggcccaacaa cattataata cattcaaaga
    28801 gaggtttgaa aattatcctt tgagagtaga agttttaagt aggtttaaaa caccaaaaca
    28861 acaaaaacag attattgaag atgctaaaaa aggattagta gatattttaa ttggaacaca
    28921 tagaataata tccaaagata taaatttacc aaacttaggt cttgtagtca ttgatgaaga
    28981 acaaagattt ggagtaaaac ataaagaagc tttaaagaaa attaaatcta cagtagatgt
    29041 acttacactt tcagcaactc ctataccaag aacattacat atgtctttaa gtggaataag
    29101 agacatgagt gttatagaag aacctccaca agaaagacat cctgtaataa catatgttac
    29161 agagagtaag gaaagcgtaa ttcaagatga gatagagaga gaattaagta gaggtggaca
    29221 ggtatttttt gtatacaata gagttgaaca cattgaagaa atggcaagta tgattcaaaa
    29281 actcgtacca gatgctagag tggctgttgc acatggaaga atgacttcta aatcattaga
    29341 aaatataatc ctaggatttt taaataaaga ttatgatgta ctagtctgta ctacaataat
    29401 tgaaactggt atggacatat caaatgcaaa tacaatgatt atatatgatg cagataaaat
    29461 gggattggct caactttacc aattgagagg tagggttgga agaagttcaa gacaaggata
    29521 tgcatatctt ttgtatgaaa aagataaaac tttaagtgag atagcggaga agagattaaa
    29581 agctatcaga gaatttacag agtttggttc tggttttaaa atagcaatga gagacctcga
    29641 aataagagga gcaggaaata ttttaggttc tcaacaacat ggtcatatgg cagttatagg
    29701 ttatgatttg tacgttaaaa tgcttaatga tgcaattaaa aaagtcaaag gtgaacctat
    29761 tgtggaggaa attgatgttg aaatagattt aagtgtaaat gcatacattc cagataatta
    29821 tatcaaagat gagttgatta aaattgaaat gtataagaaa atagcttcta ttgaaaacaa
    29881 agaagacatg ttggatatac aagaagaact tgaggataga ttctctgata tacctaaacc
    29941 agtccagaca cttcttacaa tagcttatat aaaatcgtta tgtaagatat taaaaataga
    30001 aaagataaga caattaaaag atgaaatatt attagttcca ataacaaagt atagaactaa
    30061 acagaaaata ggttataata tagttacaga attagaagaa ttattagaaa agatgtgcaa
    30121 ggtaaaataa cttgtaaatt taagaaggga tgttttaaat ttgaaaaaag taataacatt
    30181 ggtaatagct atgattttgg tagtatctgt gactgcttgc agttctagta aaggagaaac
    30241 tgtggcaaca gttgaaggta caaagatatc atcagatgaa tttaaaaaaa ctatagctct
    30301 ttataaagat tctatggaac aaacttatgg aaaagatata tgggataagg aagttgaaaa
    30361 gggtgtaaaa tacaaggaca agtttaaaga tttaattctt gaccaactta taactacaga
    30421 agtaatatat tctcaagcta aaaaagataa cttacttcct aaaaaagagg atgttgaaaa
    30481 aagttttaaa gaattaaaag acgctatggg taaagatgaa aaatataaag aacaacttaa
    30541 gaaattaggt atagatgatg agtttttaag agaccaacaa gagaaagatt tagcaatgca
    30601 aaactatcaa agtaactttg ctaaaaagac taaaatatca gatgaagaaa tgaaaaaata
    30661 ctatgataca cataaagatg aatttaaaaa agatgaggta gaagcttctc atattttatt
    30721 gaaaacagta gatgataata acaagccact ttcagataag gaaaaagctg aagctaagaa
    30781 aaaggctgaa gaggctctta aagaagttaa atcaggtgag gattttgcaa aagtagcaaa
    30841 aaaatattca caagatactt cagcaagtga tggtggtaaa ttaggattct tttcaagggg
    30901 gcaaatggtt gctgaatttg aagatgctgc tttttctatg aaaaagggtg aagtatctga
    30961 tttagttgaa actcaatatg gataccacat aattaaagtg acagacagaa taaatgagca
    31021 aacatcattt gaagatgcta aagaaactat aaaagaccaa ctgttgaaaa ataaatatca
    31081 agaacaaata gaaaaattaa ctaaagaagc taaggtagaa aaagatgaaa aagtaataaa
    31141 taaaatcaca atctaagact taaaaaaaga gtaatatgct taaaaaagct atatctggat
    31201 aagatatagc tttttttgta tatttttctt aacaatggta aaaataaact taaagtaatt
    31261 tatggaggga ttaaaaaata tgaaagcaac aggtatagtt agaagaatag atgatcttgg
    31321 aagggtagtt atcccaaaag agataagaaa gacattaaga attagagaag gagacccttt
    31381 agagatattc acagcaaaag atggagaagt catacttaaa aaatactctc caataggtga
    31441 attaaatgaa ttttcgcaag aatatgctga aacattagct gaagtactag gatatggagt
    31501 gttgataaca gacttagatt ctataatagc tgtgtctaag cttcctaaaa aagattataa
    31561 agaaaaaagt ataagtgatg agttagaagc aataataagt gaaagaacag taaaatattc
    31621 aaaagataaa aacattttac cattattcaa ggaagaccaa aaagaatata ctagtcaagt
    31681 tatcatgcct ataattagtg catctggtga ctgtataggt tcaattgtag ttgtttcaaa
    31741 ggataaagat acaattgcag aaagtgaaga aaaatcttta aaaattgctg ctaacttttt
    31801 aggaaaacaa gttcaataat tttatcataa agacctttta catgaaggtc ttttgaagtt
    31861 tttgtgtaaa atattgaata tagcattaaa aatatggtat attaaagttt gttatataaa
    31921 cagtatggag gttatgttat gaacaataac aaaaataaag actcctttct aaaaggagcc
    31981 cttatacttg ggctagcagg tgtaatcgta aaaataatgg gtggattttt taggattccc
    32041 cttggaaaca tgattggttc agaaggaatg ggatactatc aggctgctta tcctgtgtat
    32101 actctatttt taacattagc tactgctgga tttccaacag ctgtggcaaa attggtttcg
    32161 gaaaaagttg caattggaaa ttttaaaggt gcaaataaga tatttaaggt atcacataca
    32221 gttttattca ttaccggtat aatatcattt tgtatattat tttttggagc cgactacata
    32281 gtaacaaacg taatgaaaaa tccaggtgcg ttatactcta tgaaagccat tgctccagca
    32341 ttactatttg ttcctgcaat gtctgcatat agaggatatt ttcaagggag acaagatatg
    32401 actaagattg ctgtgtctca agttgcagag caatttttta gagttgtatt aggtcttact
    32461 ttggcctact tgcttatgaa gagtctaggt cagacttttg gtgcagcagg tgctatatca
    32521 ggggctacaa taggttcaat agcatctatg ctttatttgg tgtttgctta tatgctaggg
    32581 agaaaagagc gtagagcaga gatagatgca agccaaagat ttaaagatga aagagtgtct
    32641 tatattttta aaaagctttt aactgttgcc attcctataa caataggagc atcagtaatg
    32701 ccacttgtaa atatgataga taatgttatt gtaataagaa gacttatgga agcaggattt
    32761 acatacaaag tggcaaattc aatgtttggt caattaacag gtatggcaat ggccacaata
    32821 aatttaccag cagtcataac aactgcaatg agtatgagct tagttccagc aatttctaaa
    32881 gcatatgcat taggaaataa gtcaaaagct agaaaagata ctaagagtgc agttaaggta
    32941 actttactta tagtattgcc ttgtgcgttt ggtatggctt ctttagcaat accaataatg
    33001 ggtcttttat ttccacatga accatcttct gtaggaacta ttttgtttac tcttactcca
    33061 tgtgtattat ttttaggatt aatacagaca ttgactggta taattcaggg aatgggaaaa
    33121 ccaatagtac ctgtaattgc tttatgtgtt ggtatgctat gtaaaattgt aataagttat
    33181 actttaacag gtataccaga tattaatgta ctgggttcag catttggtac agttacagca
    33241 tactttgtgg cagccatgat taacttacta tatgttaaaa aacacatgaa tgtaaacttt
    33301 tctaaaaaag aatttataat aaagccattt ataacagtta tgacaatgtt tataatggtt
    33361 aagttgagtt atggtgcttt agttggattc ttaggaaaca gcatatctac aataattgca
    33421 atatgtgttg gtggaattgt atatgtagta gtaatacttg gaataggtgg aattaaaaaa
    33481 gaagagattc taactatgcc aaaaggtgat aaattatata aattgttaaa aaaagttaaa
    33541 cttataagat aatataggaa tatatatgca aataaaggaa atactataca aatagtaaat
    33601 ctccttattt gtataataat ataaaaataa agtgaaattt gctaatagga gtgatgttat
    33661 gggtaagata agtattgtag gtcttggacc aggagactat tctcttataa gccaaggtgt
    33721 gttagaatct ttaataaata gccctaaagt tttttttaga acagagaaac atccaataat
    33781 tgataaactt aaagaaacta tacaatacac ttctttagac tacttttatg aaaaagagga
    33841 aaattttgat aatgtttatt tgaaaatatc tgaatttatc atagaagaat cagcaaaggg
    33901 tgacctagtg tatgctgttc ctggtcatcc aagagttgca gagaagactg taagtataat
    33961 agagagatta gccaatgaaa agcacatagc agtagaaata ttgccttcta tgagctttgt
    34021 agacgctatg tttaactatt tggctataga cccatctgag ggatttaaac tggtagatgc
    34081 ttttgaaata gaaaattcat atatagactt ggatacaagc atgataataa ctcaggtata
    34141 tgataaattt atagcatcta atgtaaagtt aaaacttatg gaacattatg actataatca
    34201 agaagtatgt atagtaaatg gagcaggtgt caaagattta gaaagtaaaa agtttataaa
    34261 gctttgtgaa ttagacagaa atgaaaactt ttttgactat ttaactagct tatatatacc
    34321 caaaagttct aaaaagatgt ataatacagt acatgactta gaaataatag ttaatacact
    34381 tagaagtcca tctggatgtg aatgggataa aaaacaaaca catcaatcat taaaaaattc
    34441 tgttatagag gaagcatatg aactttgtaa tgcaattgat aataatgata tagatgaaat
    34501 ggtagaagag ttaggtgatg ttcttttaca ggttatattc cattgtcaga taggaaatga
    34561 ggagggctac tttgatttaa aagaagtagt aaatggcata tgtaaaaaat taattcatag
    34621 acatcctcat atatttagtg gtgcaaaatt agatatgtct aagtttgaga aaacttggga
    34681 agaattaaag aaagaagaga aaggcgaaag tactataaca gatggcctta gaaggatacc
    34741 aaaacattta ccagctctta tgaaggctag taaagttcaa cataaagcag cacttgtcgg
    34801 ctttgattgg gacaatgtag aagatgtatt taaaaaaatt gaagaagaat atcaggaatt
    34861 acttgacgaa cacagggtcg ggaatataaa atacataaag gaagagctag gcgatttatt
    34921 attttcgata gtaaatctag ctagattttt agacatagac tcaggagaag ccttgaactg
    34981 tactacagaa aaattcataa atagatttga ctttatagaa aaaagtgcaa tgaacttgaa
    35041 taaaaaatta gaagacatga cacttgaaga gatggatgag ttttggaatc aggctaaaaa
    35101 acaataaaat tgtggaagga aatttgtcaa aaatctagaa cttttatcac aaagatagaa
    35161 gtctatatta taaacatttt taggaggtaa atatcgtgaa taaagctgaa ttagtatcaa
    35221 agatggcaga aaaaagtgga ttaacaaaga aggaagcaga agctgcgtta aacgcattta
    35281 tgagttctgt tcaagatgca ctagtaaata atgaaaaagt tcaattagtt ggatttggaa
    35341 catttgagac aagagaaaga gctgctagac aaggaagaaa tccaagagat ccagagcaag
    35401 ttatagatat accagcttct aaagcaccag ttttcaaagc tggaaaagga ttaaaggata
    35461 taataaatgg ataattagaa atcttgtgaa aaagtgtgga taaatttgcc acactttttc
    35521 tatagaacca aggaaggtga taattatgag attagataaa tttttaaaag tatctaggat
    35581 tataaaaaga agaactgttg ctaaagatgc atgtgataaa gggattgtca ctataaatgg
    35641 aaaagttgct aaatcttctt cagaggtgaa tattggagat acattagaaa tacaatttgg
    35701 agagaagaag atgaagttta agataaatga agtaaaagag catgtattaa aaaatgatgc
    35761 aaaagaaatg tacgaaattt tagaataaaa ttaatagtcc tctagtatat taatagtagt
    35821 attattgaaa tatttactgg ggggatatta tggaacagaa cataactttg aaagatagat
    35881 caaagctggt tatttcggga gtcgagcaca tatactcttt taatgacaaa agagtagagc
    35941 tcaaaacttc tgtaggcgag atggtaattg aaggcgaaaa cctagatatg agcaagctaa
    36001 gtatagacga aaatataatt agcatagatg gaactataaa ttctatggta tatgcaaagc
    36061 ctaaaaagcc acaagagagt ttcttgaaaa aggtatttag atagatgata ccttttactc
    36121 aagatgtaag cattttttat gctactattt atggtggaat actcataggg gtgttgtttg
    36181 atttttatag agggctaaga gggaatttta aattcatcaa ttattttgct ataatctttg
    36241 atgtattgtt ttggtttcta gctacggtta taatattcgt aacaataaac cttacagaat
    36301 tttttgattt aagatattat cattttgtgg ctttgtttat tggttttatt ttatactata
    36361 acaccataag taaaattgtt ttatcaatta tcaataaaat aattcgtttt gtgagaaatt
    36421 cttttaaaaa agttacacat tacatagtca gttttttaaa taatttgtat tacgttataa
    36481 tctattcact acacctacta tttgatatta tattttatat accaaacata tttatagcaa
    36541 caagaaaaag tataaaaaga aaatcaaata aaaaattaaa gaacaagaaa aaatcaaaac
    36601 ctaagaagaa aaaaaataaa actaaaaaaa gggtgtagga aaaatgaact taagaaaaaa
    36661 attttcagga cagattattg ttatcagttt atttttagga atatcaattt ttagtatgat
    36721 gacaggattt gtatttgagt atacaaaagc taaggaatat aaaaaagaaa tagcaagtct
    36781 aaataaacaa ttaaaaaaga ctgaaataca aataaacagc ttaaagaaag atgaaaaatc
    36841 atatgaagga gacttagagg atatagcaag aaaaagattg aatatggtaa aaccaaatga
    36901 aacagtttat gtagacataa atcgttgatt aaaacttgct aataagttta cagatgtatt
    36961 gattttaaaa tttaagattt aataaaaaag tcattcagtt aaggatggct ttttgttttt
    37021 attgaggagg gaatttcata atgaaagtag gagctataga tattgggaca aactcaatga
    37081 gattactgat agcagattat agagatggga attttataaa cagaaaaaaa tttgttaata
    37141 caactagaat cggttatggt gtggatagta aaggatatat aaaagaagat gctatcaaaa
    37201 gaaatataaa agcattaaaa gaattttctg ataagtgtaa tgatgaaaag tgtgaaaaaa
    37261 tttattgtat ggggacatct gctcttagag acagtaaaaa tggagattac tttgttaaat
    37321 tagctaaaga agaaactgga atagatgttg atatagttac gggaatagaa gaatcaaatt
    37381 tgggctttaa gggtgtttta gaaggaatac aaggtgatga tactattatt gttatagata
    37441 ttggaggagg ttcaacagaa tttattgtag gtgattcaga aggaattaaa tttaataaaa
    37501 gtgagaatgt aggtgctctt aggatgacag agaggttttt gaagcacgat attattgaag
    37561 atgaagagtt tgaagctatg agttcattta tatatgatga gattgacaaa acgttaaatc
    37621 atataaaaga cagaggatta aaaactctta taggtatagg aggtacgatt acatcattat
    37681 cagcaatgaa tcaagaatta gagatttact ctatggaaaa aatacataat agtaaaatac
    37741 acaaaaaaga aatagaagaa attctacaaa aattaataaa gatgacatta agtgataaaa
    37801 agaacttaaa agggctacaa gaaaaacgtg cggatattat aactgctggt gtaaaaatat
    37861 tgaatataat aatggaaaaa ttagaaatag aagaaataac cgtaagtgaa tacgataatt
    37921 tggaaggctt aatatgtcaa aagtcaaaaa aaatgtatta atattttttg atttttaaaa
    37981 ccgattatga caaaaaaaat tactcctctt tgttacaatt cttaatataa acaaaagagg
    38041 aggttttttt atgcaaagaa gtgtagcagt tgtgaagaat aagactgtaa aaccaaatgt
    38101 cataaaatta ataagcggat atattgattt tactacggcg gcattcgctg tgatgggatt
    38161 tttacttagt aggtctatta tagtagactc tgtagctcct ttaggaattg ccttttttat
    38221 atgtgcatca aagattgata agtacaagat acctgtattc atatcaacat tattaggaat
    38281 attgttatca tctaatgatt ttggaaatgt agcaaaatat gctgtgtgcc ttacaatatt
    38341 tatggctatg aataaaaaat taaaattatc agattcaatt gctaaggttg catttatagg
    38401 tgcaataata ctacttccaa tctccatggg acaagctttc ttttcaaata ggtatgtata
    38461 tggtcttctt atagcaggag tagaagcaat aataatgttt atatctatat atgtgttttc
    38521 ttatgggata ggtttaatgg ccaatgtcaa caataggatg tctgtacgga cagaagaagc
    38581 tatatcaata agtttactgc taacctttag cataatggga ataggagata tatctctgtt
    38641 tggaatatca ataaggacca ttttatcgac cgtattgata ttgctgacct caatagtagg
    38701 tggagctacc atgggagcat ctagtggtgt aattattggt gtagcatcca ttttaaataa
    38761 tatgacatca gctgtttata tggggatata ttctttttca ggattaataa gtggagcatt
    38821 taataaaata aataaatatt tctgtatact aggatatatt ctaagttgga caataatata
    38881 cctatatact tctggtatta cttctaatat gatgcaatta agagatatac tattaggttg
    38941 tttgatagta ctagtattgc cagagagatt atttaataag attgaaaaat taataaaaag
    39001 taatgtggct tcaaatgaaa tcgtatacga ttatattatg agaagcaaaa acctaactaa
    39061 tagcagattg aacagtattt ataaaactta tgatgacctt gcagatacat ttgataagat
    39121 aagagaaaaa gacaaagtgc tagaccaaag agatatagct aatgtaatag atatgattca
    39181 taatgatgaa tgtaaaagtt gtagtatgag aagaatgtgc tgggagtcaa gatttaatca
    39241 tacatacact atgatatata acattcttga aaaaattgag gagaaagggg agttaagtct
    39301 aaacgatata cctaaaaatt ttagaaagga atgtatgaaa ccagagtcga tagtgaaaat
    39361 atcaaatcac tattacaaaa tgtttgtatt agattatgat tggagtgtga agttttcaga
    39421 aagtagaaag ttaatagcaa atcagataag aagtatttca aaatccatta aatccttatc
    39481 tcaagattta gaaggtgaca taatgctaga tatagaaaag gaaaaaaata tatatgaaca
    39541 gttagaaaga tatgatataa cagttgataa agtaagttac ttaacaaaaa gtaacaatga
    39601 gtttgaaata tcaatagaaa agaaaacttg tcatgatgga tgtatgtgtg aagataaaat
    39661 tgtaaatata atttcagacc tagtaggtga aaatatgtct gttagaaaaa taggatgtca
    39721 ctgtttagga ggtaaatgca aggcaacatt cgttaaatct caaaaatata aggctgttac
    39781 agaagtttca gcaatgtcaa gggatgggca tatcctttgt ggagacaatt acacatacat
    39841 ggagataaat gatggtaaat acatgatggc tataagtgat ggtatgggaa agggtaaaaa
    39901 agcatatgaa gaatcttctg ccactattga tatactggaa aaaatgatag atgcaaaaat
    39961 taaagatgag atagtcattg atactataaa taatatgttg ttgttaaaat cttcagaaga
    40021 gatgttttca actctggatt tgggaatttt agatttaaaa cgaggttgtt tagaaactat
    40081 aaagatggga gcgtgttcta cttatataaa aagagaggat ggagaagttg acttaatatc
    40141 atcatcatca ttacctgtgg gtatattatc agatgtaaaa attgatagaa aaaatgtaaa
    40201 agttaaggaa ggagactatg ttataatggt ttctgatgga attgtagatg caggaagaaa
    40261 taacaattta ggagacaatt ggttaatata cttcttaaag aatatagaga ctacaaatcc
    40321 aaaggaaatt tcaaatttaa tattggatag agcattagag ttacaagcgt tacaaatcga
    40381 agatgacatg actgttttag ttacgaaaat atgtacaaaa taaggtttac aagattatgt
    40441 gtataagatg tggataactt gtgtataata tgttaataga cttttgaaaa aagtatagtt
    40501 attaacaaaa ataacatata aatcaacaaa atttgttgat aagtatggca gaaatgttaa
    40561 taaaatgtaa aaaaagaaaa aacaaaagct attatcaaaa gagattcatg ttgaaataac
    40621 taaatttcta agtaaagaaa acctgtgaat atgttgatta ttatgtggat aactattaat
    40681 tcgatattta tttatatagt atgaataatt gaataatata tctaaaaaaa gccatcctat
    40741 tattaagtaa atttattgaa aggatggctt tttatgaata gaaaattgtg caaagcaata
    40801 atacctttat ttatttctat tttttgcatg ttaataataa gtataaaagc tgaccaagga
    40861 tatcaaaaga ataatgacag agcaattgct gtcatgaata ataatgatac tgaagatgat
    40921 gctgaaaaaa aacaagaagt agactatgaa aaaacatgtt ggaatttgca atctttgttc
    40981 aaaagtgatg accaatggaa aaaagaactt aagagttttg ataaagatac taaagagttg
    41041 aaaaattaca taggtaaagt aacaaaatcg ccaacacatt tgtcttttgc cttaaacatt
    41101 aaggaaaaac ttgatataag actaaataaa ctttctgcct atgtaaaatt aaaacaagat
    41161 actaataaaa attcatataa gtacttagat atgagtgata gtatgagtaa atcatatggg
    41221 aattatatgg ggatttgttc agatttagaa ttagaaatac taaaattatc aaacaaagat
    41281 tacaagaaaa ttatttcaaa taaaaatata aataggaaat atggtgcata tttaagagat
    41341 ataagaagga ataaagtaca ttatttggat gataaatcag aagatattct aagcaaagta
    41401 tcttcaataa gtagtcttcc aagccaagta tatgaattat ttagaaatat ggatagaaaa
    41461 actaatttga caccagcgca atatgcaagt gaattagagt cagtagatag agaaaataga
    41521 aaaaaagctt atcaagatga acttataact tacaatgata atataaatac aattgcaggt
    41581 ttaattactg gtcaagttaa aaagaatatt ttttattctc aggaaagagg ttatgagtct
    41641 tctttagaaa tgtacttgga atctgataat gtagatgaaa aagtatacaa taatttgata
    41701 aggactgtaa atagtaatat ggacagtcta cacaaatata tagatttaag aaaaaaagta
    41761 ttaaaattag ataaggttca ttcatatgat atgtttgtgc ctatggttaa ggctgttgat
    41821 aataatatta attatgaaaa ggcacaaagt atagtatatt cagcgttgtc acctttagga
    41881 aaagaataca atgatgtagt atataaagct ttcaatgaaa aatggataga tgtttattca
    41941 catgaaaata aagtaagtgg tggatactgc ttatctgttt atgaaaatca tccatatata
    42001 cttttaaatt ataataattc attaggttca gtttcgactt tatcacatga gcttggacat
    42061 gctgtatatg aatacttaag ttctaagaat cagaattatt ttaattcaaa tccgtctata
    42121 tttactcacg aagttgcatc tacaacgaat gaagcattac tttacgagat gcttataaaa
    42181 gaagctaaag gcaatgatga aaaagcttat tatataacac agtatttaga cttgataaaa
    42241 gatactcttt atacacaaac tatgtatgct gaatttgaaa aaactataca cgagttagta
    42301 gaaaaaggaa aaagtgtaaa cgcattagta ttaaatgatg tttggggaca acttcttaaa
    42361 aaatattatg gacaaagcta tgaattagac caattatcta aggtcggatg ggctagaatc
    42421 ccacattttt ataatagttt ctatgtctat aaatatgcta caggttgtag tgcaggagta
    42481 agttttgccc aagacatcct aaagaatggt tcagataact atatgagctt tcttaaaaag
    42541 ggaagttcag attatccaat aaacttgtta aaatcaagtg gcataaattt aactaataca
    42601 aaacccatag aaaatacaat caaaaaattt gatagtttag tagcagaact tgaaaagcta
    42661 ttataaacat tattgtaatc cacatggtaa atatatcgaa ttaatgataa aatatactaa
    42721 aagtttaaag tcctatcaag ataggacttt ttatttatta attagtggga gatgattcta
    42781 tgcaagttaa agttaaaaag gctgtaatac cagcagcagg tttaggaact agatttttgc
    42841 cagctacaaa ggcacaacca aaagagatgt taccaatagt tgataagcca actctacaat
    42901 acataataga agaagctgtg gcctcaggaa tagaggaaat actaataata acaggtagaa
    42961 ataaaaaatc tattgaagac cactttgata aatctgtaga attggaactt gatttagaga
    43021 aaaaaggtaa gaaagaattg ttggaggtag tacaaaacat atctaacatg attaacatac
    43081 attatataag acaaaaagag ccaaaaggat tgggagatgc aatttattgt gcgaggcatt
    43141 ttataggtga tgagccgttt gcagtaatgt taggggatga tattgtagat aatgatgtac
    43201 cttgtctaaa acaactaaca gatgcatatg aggaatatag aactacaata ttgggtgttc
    43261 aaaaggtaaa tcaagaagat actaataaat atgggataat agaagcaaaa aatatagaag
    43321 gtagagttta caaggtaaaa gacatggtag aaaagccaga gtcaggaaaa gctccttcaa
    43381 atattgctat tttaggtaga tatataataa ctcctgaaat ctttgatatt cttaaggatt
    43441 taccaccagg taaaggtgga gaggtacaac ttacagatgc tttaaaaata ttatctaaga
    43501 aagaagctat gtatgcatac aattttgaag gcaagagata tgatgttggg gacaagctag
    43561 gatttttaga agctactgtt gactttgctc taaagaaaga agaccttaag gaagatttta
    43621 taaaatattt gaaacatgta tgtagtgaat ttgataaaaa taacaatgtg ttatacaata
    43681 atacagatga aaaaatagat ttaattgaag tcaatgaagc tagagaactt gaaattcaaa
    43741 aataattaat gaaaggtgat gtatggtggc aaaatcaagt cagaaaagaa ttaatagaat
    43801 aatagctgtt gggattgtgg gaatcctcgt aataacgagt ataacagctg ttttgagtat
    43861 gattgcaact atgtaataag ataaatgaca gcagttgata tacataaata taaaaaccat
    43921 atataacttg atgtagtcaa aaacttacaa tattttagtt atatatggtt tttagaattt
    43981 ataaatgtaa aaaaaaattt ttataatagt tgcatatttt
//
LOCUS       DAELXX010000021        43359 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.021, whole genome shotgun sequence.
ACCESSION   DAELXX010000021 DAELXX010000000
VERSION     DAELXX010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43359
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.021"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(895..1305)
                     /locus_tag="KPH29_001089"
     CDS             complement(895..1305)
                     /locus_tag="KPH29_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466714.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKLESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYAAID"
     gene            complement(1521..2903)
                     /locus_tag="KPH29_001090"
     CDS             complement(1521..2903)
                     /locus_tag="KPH29_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG1466715.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNTSTENYTLKKASDSTGKNNSDEFEIMSNIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKSGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEETYVNKSSNSKDVLYTDSGEVSIEQSNYNDNRFVISRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(2914..3273)
                     /locus_tag="KPH29_001091"
     CDS             complement(2914..3273)
                     /locus_tag="KPH29_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG1466716.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
     gene            complement(3478..4257)
                     /locus_tag="KPH29_001092"
     CDS             complement(3478..4257)
                     /locus_tag="KPH29_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBG1466717.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTIISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
     gene            complement(4718..5329)
                     /locus_tag="KPH29_001093"
     CDS             complement(4718..5329)
                     /locus_tag="KPH29_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG1466718.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
     gene            complement(5421..6410)
                     /locus_tag="KPH29_001094"
     CDS             complement(5421..6410)
                     /locus_tag="KPH29_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBG1466719.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYVEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NRESREKFAEEMNLKLSKYNTKIVAVESSDEAIDNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            complement(6477..7340)
                     /locus_tag="KPH29_001095"
     CDS             complement(6477..7340)
                     /locus_tag="KPH29_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HBG1466720.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            complement(7446..8042)
                     /locus_tag="KPH29_001096"
     CDS             complement(7446..8042)
                     /locus_tag="KPH29_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HBG1466721.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGANGLLKMKENGLFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            complement(8082..8885)
                     /locus_tag="KPH29_001097"
     CDS             complement(8082..8885)
                     /locus_tag="KPH29_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HBG1466722.1"
                     /translation="MIKLTDEEFIVLVNHVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDKTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKSFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            complement(8921..9361)
                     /locus_tag="KPH29_001098"
     CDS             complement(8921..9361)
                     /locus_tag="KPH29_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBG1466723.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            complement(9374..11476)
                     /locus_tag="KPH29_001099"
     CDS             complement(9374..11476)
                     /locus_tag="KPH29_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HBG1466724.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESTDEKEAIDSNFEYKED
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKNTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKNVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQINEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMHGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            complement(11733..13436)
                     /locus_tag="KPH29_001100"
     CDS             complement(11733..13436)
                     /locus_tag="KPH29_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HBG1466725.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINRV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            complement(13462..14421)
                     /locus_tag="KPH29_001101"
     CDS             complement(13462..14421)
                     /locus_tag="KPH29_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG1466726.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNTRTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            complement(14557..15075)
                     /locus_tag="KPH29_001102"
     CDS             complement(14557..15075)
                     /locus_tag="KPH29_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-binding chemotaxis protein CheW"
                     /protein_id="HBG1466727.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFKVDEQDFGVELEKVIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            complement(15118..15603)
                     /locus_tag="KPH29_001103"
     CDS             complement(15118..15603)
                     /locus_tag="KPH29_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HBG1466728.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            complement(15600..16217)
                     /locus_tag="KPH29_001104"
     CDS             complement(15600..16217)
                     /locus_tag="KPH29_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HBG1466729.1"
                     /translation="MINYLELDDVHIDALREIGNIGSGNAITALASMINSNVEVLIPM
                     VKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEAF
                     LDEIEAIEISAIKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVPMIRF
                     SDVGDKVLFIENKFKMSDNYFTSHILMIPEMSSLREILVRLGLEV"
     gene            complement(16283..16645)
                     /locus_tag="KPH29_001105"
     CDS             complement(16283..16645)
                     /locus_tag="KPH29_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBG1466730.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVNNVLK"
     gene            complement(17389..17886)
                     /locus_tag="KPH29_001106"
     CDS             complement(17389..17886)
                     /locus_tag="KPH29_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBG1466731.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAVNAIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVILAGKYAKFVIVGGQKEVGDFWFKFWQMDFDRTYISDFEEYQCNTFDTKKQEIHI
                     YIGIK"
     gene            complement(18061..18972)
                     /locus_tag="KPH29_001107"
     CDS             complement(18061..18972)
                     /locus_tag="KPH29_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBG1466732.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
     gene            19897..20748
                     /locus_tag="KPH29_001108"
     CDS             19897..20748
                     /locus_tag="KPH29_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBG1466733.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
     gene            20752..21222
                     /locus_tag="KPH29_001109"
     CDS             20752..21222
                     /locus_tag="KPH29_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466734.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            21235..22545
                     /locus_tag="KPH29_001110"
     CDS             21235..22545
                     /locus_tag="KPH29_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG1466735.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKEEYLTFLEKHDKTL"
     gene            22757..23503
                     /locus_tag="KPH29_001111"
     CDS             22757..23503
                     /locus_tag="KPH29_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG1466736.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     IKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCNSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTADSI
                     SLLIDDGVLFCGDSAMNGFPSTHKITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     EYSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            23865..25406
                     /locus_tag="KPH29_001112"
     CDS             23865..25406
                     /locus_tag="KPH29_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBG1466737.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKAGISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            25441..26547
                     /locus_tag="KPH29_001113"
     CDS             25441..26547
                     /locus_tag="KPH29_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG1466738.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGEKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVTTEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFHGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DPLTTSNIGKIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHSMSYPSFELSRDSHVFKLSEEAIRQVGVVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
     gene            complement(26992..28278)
                     /locus_tag="KPH29_001114"
     CDS             complement(26992..28278)
                     /locus_tag="KPH29_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG1466739.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAISENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(28401..31499)
                     /locus_tag="KPH29_001115"
     CDS             complement(28401..31499)
                     /locus_tag="KPH29_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG1466740.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGINDISHVK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRKKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKFKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFIVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMEVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKHLIHPDDIILFDDNHLLKSLDSLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDILTGLYN
                     YESFCEQTSLMLKRYPDKSYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDVHKLIYKIKNSLNRKKFEYMLVPCFGVYKILDNN
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLRHTIAMAKDLDMDVVAEGVETKEQAEFLVSLDCVVIQGYYYCKPIPSADFEKLLY
                     QK"
     gene            32223..32375
                     /locus_tag="KPH29_001116"
     CDS             32223..32375
                     /locus_tag="KPH29_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466741.1"
                     /translation="MKIFSFLLFVVGICFCIAGFMGHYPAFAGATLSFFATTMIAVRQ
                     VKTKKK"
     gene            complement(32667..33371)
                     /locus_tag="KPH29_001117"
     CDS             complement(32667..33371)
                     /locus_tag="KPH29_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1466742.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(33362..34969)
                     /locus_tag="KPH29_001118"
     CDS             complement(33362..34969)
                     /locus_tag="KPH29_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HBG1466743.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(35223..36401)
                     /locus_tag="KPH29_001119"
     CDS             complement(35223..36401)
                     /locus_tag="KPH29_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466744.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVGAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(36627..37121)
                     /locus_tag="KPH29_001120"
     CDS             complement(36627..37121)
                     /locus_tag="KPH29_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG1466745.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNMGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(37122..37883)
                     /locus_tag="KPH29_001121"
     CDS             complement(37122..37883)
                     /locus_tag="KPH29_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG1466746.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
     gene            38606..41440
                     /locus_tag="KPH29_001122"
     CDS             38606..41440
                     /locus_tag="KPH29_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG1466747.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHVSIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIVHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNISKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNIRKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
     gene            41483..42727
                     /locus_tag="KPH29_001123"
     CDS             41483..42727
                     /locus_tag="KPH29_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG1466748.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVAKYYNDSGKFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLEIKNSVCFKV"
     gene            complement(43168..>43359)
                     /locus_tag="KPH29_001124"
     CDS             complement(43168..>43359)
                     /locus_tag="KPH29_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HBG1466749.1"
                     /translation="VGSKATYKTAIDEAQAILDKADATQKEIDDAVTALNTATATFEA
                     GKVPTTIALMLSRILGFMK"
BASE COUNT        14617 a         7204 c         4915 g        16623 t
ORIGIN      
        1 aattatctat tttttctgat attcttatct accattatct atatatttat tttacgatta
       61 ctctttcacc ctaacccttt aattatctat tttttctgat attcttatct accattatct
      121 atatatttat tttacgatta ctctttcacc ctaacccttt aactatctat ttttttctgg
      181 tattcttatc tactattatc cgtatattta ttttatgatt actcttttaa cctaccccct
      241 atactttata gtaatatgtt tttaaccttt ttcattattc ccaatatgct ttataactac
      301 aaatactctc cccccttaaa aataataata aaattaatca ataaatttat aatcatatta
      361 ttactctatg tattaagcaa ttactatgcc aataaatttt gattttttta aacaaatctt
      421 aaaaatccta aaatgctaat tttctttaac ttttaatgaa tctaatagat aatatttaat
      481 tttcgttata tttttcagaa tgtttcaatt attattttta agaaaaaaaa ataatgttaa
      541 catttcttaa catacattaa aaaatgttaa cattataaat ttggtttaat acttttactt
      601 tgactcgatt tacttttatt ctacttctta ctttattttt aacttagact ttattttttt
      661 attacttatt tgagtcttat ttattttatt ctcactccat atgttcaatt acatttgtct
      721 aaattctttg atacttttat tacataattt taacaagctt aagtgttatt tgggtagtgc
      781 agttgataat tatttaaata atcatcaact gcatgtttat atcattacag tagcttaatg
      841 tatttaatca tacttactta ggattttgaa atatcttaga gattatattt aacattagtc
      901 aattgctgca taattgtaag caattgaaat ttgagaccca tcttcatttt ctatagtgat
      961 aggcttcata atttttaatc cttcagtttt tatcttttct aattcttgtt ccattttctt
     1021 aattgtttcc tcaaggtctt tttgacttac atctttgact gcattagggt ctttagcata
     1081 gtcttttaat tcttgaatat gtttagcata ctcttcataa gtataatgct caacaccata
     1141 cttttccaat tcagtagggt cagttattcc tgattttatt agagattcaa gtttaggtaa
     1201 agcaatatta gatgaatcat ttccatttgc aaatactgct ccagttcctc ctagtaccat
     1261 tgcagatacc atcattgcac ttataaggga tttttttatt ttcataacca atcttccttt
     1321 cttagagatt tatttgggta gtgtagttta aaatgactat tgcaatagtt atcaaccaca
     1381 tctttagact accatagtag tcatatttgt gtcaagtctt tttaaaaaac ttttcaataa
     1441 aaaatactat ctcaaaacaa aattaattat tttgagatag tattttaatt ctaaatcttt
     1501 tatattacta agatttatgg ttactctatc catgttctat attgttcttt attaactgct
     1561 aacaaattcc cttttcttga tatgacaaag cgattatcat tatagttact ttgctcaatt
     1621 gaaacttctc ctgaatcagt ataaagtaca tccttactat tactagattt atttacatac
     1681 gtttcttcac tattctcaga attagttact ttgctcatag atgattcttc atgctctcta
     1741 aattcaatta cagatacttt tgccgtacta aaatgagtct ctccatcact tttagcttta
     1801 ctaacaagaa tatcagattc tactttggga gaatataaat cagttgattt actacttatt
     1861 gaaaatccac ttttatcact ttgttcattt tttgttaaac caaaagattc aacctcatta
     1921 atctctttat tttctgttgc aaaaacaaga cctgtcccac aaactaatac agttgaaact
     1981 aaaaaagtaa cataagttga ttttttaaat ttcataatat taactattct cttttctatt
     2041 tttgtctttc caaatccact acatattgtc attgaaaatg gattattctc ctctgctaga
     2101 cttattagtg tggttgcata atcagcccta tattcttcac caagtatact cataaccttt
     2161 tcatcacaac taaattctat gtctttattt agtaaaaaaa acatcaccca aactaaggga
     2221 ttaaaccaat ggacaacaag agctacaagc ataaataact ttaacagatt atcaaagcta
     2281 ttaatatgaa ccatttcatg agttaaaaca aagtctaaag tttgattatt agcataattc
     2341 atttgctttg gcaaaataat tcgtggtttt aaaataccag atgtaagtgg tgtcctagta
     2401 aattcagata tgcaaacaat aaatcttcta aaacggctat tcttacacat ccatttttta
     2461 atgaactcat gattttttat atgaattgaa gttttaaaaa gattaattat tttaaaatat
     2521 ctaaagcata aaagtatgct aaacataata gtaactataa aagacaaaat gcccaacatt
     2581 tccaacatca taatattttc aatattagac attatttcaa attcgtcaga attgttttta
     2641 cctgtagagt cactagcttt cttaagagta taattttctg ttgatgtatt acccaattct
     2701 acatcaaaag tcgattcaat tatactataa atactaaaac cagaatttaa acttactggt
     2761 attagaagtt taaataaaac aatcaaccac aataatacaa ataccttttt aggtaatttt
     2821 ttaaataaac atgctcttat aaaaataact attaaaagta atactccaga agtaaaactc
     2881 atttctaaca atttaatgaa cataaatctt acctcacttt aatttttcaa caatttgttt
     2941 gagttttgta atctcatcac ttgaaagatt ttctttatta ataaaagctg aaagaaattt
     3001 ttctgaagac cctccaaaca ttttattaat taaagtttct gtttcctgtt tttgtacttc
     3061 ctctttgctt attaaagctt tacaaacaaa atttggctca attctctcta ttgcaccttt
     3121 tgacactaat ttttttataa cggtatacgt agtgtttcta ttccatcctg tttcttcttt
     3181 taaaattttt actaactctc cagccttgct ttctcctctc tcccatagaa cttccattac
     3241 tttaagttct gaatcaaata attttatctc catataaaat cgctcctcaa atatagttat
     3301 cacaatagtc attactataa taaaaaacta tcatagtagt ctatactatg tcaatcattt
     3361 attttttaat aataaaaact gtctttttaa aaaattctta taaaagacag ttttatcaat
     3421 gttatacttt aatgtttaat taaagccata ttttatttaa atgtataact atctatctta
     3481 gtacatagta taagattttt ctttatctaa tagtatatcc tctacatctg ggttttcttt
     3541 tactaattgt ctataaaaat tacaattagt aacacctaca tatggaatta gccatagtag
     3601 acctattcca agagtagcta ttgaaagtat ataccatcct ataaaactaa agtttagtac
     3661 aaataattta agtacatttc ctttcatcat atctaaacta gcacccattg atttaaatac
     3721 acccatatta tccttgtctt cacaaattaa gaaagtagct tgagaaacat ataatagcaa
     3781 tatataaacc ggtaacataa gaacaagtat tataagaact agagttattc cagtagaaat
     3841 aatagtccct attccaacat tatcacttga taaatattca attgctccac tagtaagtaa
     3901 aactccaaat gataaaaacg ctcctataat tgtgattata aaagaataaa tataaacaat
     3961 aattgattca aataaataaa aaccaaaact tcttaaataa attctcccac ttacaaagat
     4021 atctcctatc ttgacttttt catctttagt aattttcaag aacatacatg taaatcctgc
     4081 tgaaacgcaa aaagctatta cagccactac aatagtagtc aatggttcaa aaggtaatcc
     4141 tacaattgat attaatacta tcaatgcaat atatactgcc atagaaattg cacatattcc
     4201 ccagtttcca gataactgag ctttagatac tcgttttaat tcttgccttg ataccataaa
     4261 aaacacccct ttcattttat aaaaaaactt ttaaaaatat aaataatctt ttaacgtata
     4321 atattaatat atcatatttt cgactatata agatacaatt attcaccatg ttttatcaag
     4381 atatgccata attgtattac tagttttata tagtcaacat taccaaatac atcaaataaa
     4441 tttgattaat tattcaacaa gtaataaaat aatttctatt aattgtactt tatttagtaa
     4501 acaaattatt taataaaaaa attacttaat acagtaaatt aattggtaag ataaattatt
     4561 tattattagt aaagtaaatt atttactatt tatcaagata aattatttat aaaataaatc
     4621 atttataaaa tataatttat actttgaaaa taaaataaac tatcttccaa acattttatt
     4681 atatgcttaa aagatagttt tgtgtcttta aaataaatta aattgtatat attccttcac
     4741 ataacttttg tatattacta ggtggttgcc atttaccatc ttttttagta tatgtaatca
     4801 tagttgtaac ttccttagtt ggtttcttat tgtaaaggtc tataatcatt tttgtcatat
     4861 catcttgaac ttctttttct tttttatcgt aattcttttc atcagttaca taagatttta
     4921 ctaaagtgtt cgcttcactc attactttac tagtatctaa agctttactt acaactttca
     4981 aagttatctt atccttacta gaagattcca ctgttactgt cttttcaaca ccttgaatta
     5041 atttaacaat tgtttcgtat atcttttcag cattttcttt accataaaga ggtgtcaatt
     5101 gagcaatcat tgaaaccttg tccttgctgt tataagtttg aatctgttta tattcagctt
     5161 ctgttaatgc atcagaaatt cctttttcat catacttata catttctaat gcagctaaat
     5221 tagcttcaaa attcgacttt actttttctt ctgcattaga acaccctata gttagaacta
     5281 aaactagagc cataatcgta gacaatatta acttttttac cattttcata caaacatcct
     5341 ccctcaatta aaataataaa aaatttattt atcaacatta taacataaat ttgtctaact
     5401 atagaattat tttgaatatt ttaccacttc cagtctattc caataccgtt ttcttttgct
     5461 ttatcgtata ttctttttgc tgttacgaca tcttgtacac ctatcccaac agttttaaat
     5521 acaattatct catcatcatc ttctcttcca ggaatagttc catttataac gtttcctaaa
     5581 tcaccactaa aatcatcttt acttatctta ccttctttta atggaattaa tatatctcct
     5641 gactcagata aaactgcatc tgttgattca aaaaatatct tagacgctct agttaaacag
     5701 tttgggcaca actcttgcat atctggcata tatgaaccta ctgcacttat taaagctcct
     5761 tttttcacta agtttccatt aagtactggt tgttttgatg gagtagctaa aactatcaca
     5821 tctgcgttat ctatggcttc atcagatgat tcaactgcaa ctattttagt attatattta
     5881 cttaatttta agttcatttc ttctgcaaat ttttctctac tttccctgtt tctactaaaa
     5941 actcttacct catccaaatt tctagcttct atcatggctt caagttgtgg ctcaccttgt
     6001 cctcctagac ctatcaaagc tccaatctta gaatcttttt tagccaatac atctatagct
     6061 gctccagagg ctgctccagt acgaatctga gttacataag taccatccaa tactgcacta
     6121 acttctccag tttctccatc taataatagt actgttgctg gaattactgg tttgcctttt
     6181 tgagcatttt taggaaatac agatacaatt tttattcccc cacagcctaa ttcttctacg
     6241 tatcctggca taaataacat actagcatct tgacttggaa cagatatatt ggttctcaaa
     6301 ggatttacac tctttccttc acaataaatt ctaaaagctt ccttatcagc ttcaattgca
     6361 tctctcattg taaatacttt ttttatgtcg tcttttttta aaagtagcat ttttataccc
     6421 ccaattttaa taagtttcta tgctaaatat tatagcataa catttaattt aatcttctat
     6481 ttcaaatcaa ataaatctac atactctggt tttagcatag aacttatcaa cgctttattt
     6541 attgaaaatt ctggtattcc cgctgcatat ggagttatat catataaatc aaaatataca
     6601 actagattct catcttgcaa gtaaaagttt tgattctctt ttataccttt aaaattatat
     6661 attccgatat tttcttcatc ctttttttca agttcagcta tttgtctttt aatttcttca
     6721 tctataactt ctttatattt agtattatcc ttaaataagt ccattaattc taaaaatttt
     6781 ccagttctca tatccacatt atatgctata tcttgataaa atccatgtgc tccaccacta
     6841 taagtataat atctcacttt tatactaaga gcagaatcag tattcttttt aagttcaaag
     6901 tctacatttg caacaaactt atttttctcg tcaggattat ccttaagata ctgctttgct
     6961 tctttatatg atttattata aaattcaaat atgtcatttt ttatcttatc atttgtagac
     7021 ctttcaacaa ttttatttga atttgttatt acaggcatgt ttatagtact ttctaaatac
     7081 tcatcatttt tcactattgt tttacttcct attgtactta ctgataaagt ttgtttacta
     7141 tttgtattac cctttggtaa tgactggtta ctcataacag tcactgcttc cttattacta
     7201 tttaaattaa ttgaagcatc tttccttaaa ttattatatt tatagtcatg tttaattgcc
     7261 aaaacagcca ttatagtcaa tgttaaaaca aatacatatc ttaaaacttt agcttttgta
     7321 tcgactttaa aaaatttcat attatcattt tcctcctaaa tataaatctc tcttattata
     7381 tttggagttt ctagggtttt ttatccatat aatttaataa aagattaatt tttatttata
     7441 aaaaattaat ctttttgttt taaaatatta atatgtctta tcaaacagtt tgaaatttca
     7501 ccaagaggta attgtaacat aacagaacct ctatcaaaag ctaccattgg cattccatat
     7561 actatacaag aattttcatc ctgtccaatt gtaaataaac cattttcttt catttttaaa
     7621 agtccatttg ctccatcata acccatacct gtaagtatta ttcctattga atttttacct
     7681 gctacttctg ctactgaatc aaacaaaacg tctactgatg gacagtgacc acttattctt
     7741 tctccttttt cacaagttac ataatatcct tgtttatccg tacacaattt catctggtaa
     7801 cccccaggtg caattaacac atttccttgt tcgactctat cattattctt agcttctctt
     7861 acattcataa tacactgctt gtcaagccgt tgtgcataca tataagtaaa tatagctggc
     7921 atatgttgaa ctacaacaat ccctggtgtt gattttggta ggtcttttat aatttcaagt
     7981 attgcttctg taccacctac agatgcgcct atagcaataa ccatattatt aaaacgttcc
     8041 acattaccgc ctccaaaaaa tcatatatca aaaatacctt tttatcgttt atatatagca
     8101 ggttttacat atttgtattt agtactactt ctgttgatta cttctgcatg tcctacaaac
     8161 aaaaaactat cttttttggt agcattataa aatttattta taagttcttc tcttgttttt
     8221 aaatcaaaat aaatcataac atttctacaa aaaatcaaat caaaatgttg atatgagaaa
     8281 tcattcatta aattaaatga cttaaaaatt acactttttc taatcttatc acaaatttta
     8341 aatgtaccat ccttgttgtc tataaaatac ttcttcttcc ataactcagg aacattgtta
     8401 acatttgatt ctatgtatat acctctttta gctttttcaa gtgagttagt tgaaatatct
     8461 gtggctatga ttttaatgtt ccacaactct ttattatatc caaaataatc atccaaagtc
     8521 atagctattg aatacgcttc ttctccacta gaacatcctg cactccatat atttatgttt
     8581 cttattttat tattttcttc ccaaaaagga agtatactat tctgtataaa ctcaaaatgt
     8641 tgaggttctc tcataaaaaa tgtgtggtta gtactcaatc tatttattag gtttatagct
     8701 tcatttcctg tcttatcttt aaataataga ttcatatatt gactaaaaga actaagtttt
     8761 ttctctatca ttgtattgta taagcgacct tcaattaacg ctcttttttt acttaaatca
     8821 ataccatatt ctttttttac atggtttaca agaactataa attcttcatc tgtgagtttt
     8881 atcatgcaaa aacttcctcc tgctaattta tcagaactaa ctatatgcta ttaatcaaaa
     8941 actttcgaat atctaaaatt aatatagaat tatcttcaac tttcgcaatc tcttttatat
     9001 ctttattaat attagtgttg ttagaattag tcgtctctag taaatttaga ttttctatat
     9061 gaataacatc aacaacttta tctacaataa ttccaacttg atactcatca acctttgtaa
     9121 ttattataca agttctctca ttataatcag ctttaggaag attgtacttt atatgcatat
     9181 taataactgg tattattctt cctctcaaat taataactcc acttatatat gttggaagac
     9241 atggtacata cgttattgat tgaaatctta tggtttcaat cacattttct aattctattc
     9301 cataaataac atcatttaca taaaatgtta atacttgttt aataatttca tctttagcca
     9361 tactttaaat cccctaatat atttctataa tgctatctgc atctataatt aaacttatac
     9421 ttccatcccc aagtattgta catcccgata aacccttatt ttttatatta aatcggttga
     9481 aataaactgg aaatggtttc acaacaactt gctgattacc tattatttca tcaacaaaaa
     9541 tgcatactgc tttatgctca ttttcaataa ttatcatatt accatgcatc agattggtta
     9601 cttttgtttg aatattatat attttatgta accttataat gggaatacat gctccatata
     9661 ttgtaattaa ctctgtctta tttccttctg taattatttg atttaagtct gtaagtttaa
     9721 atgttcttct tatggatgat attggtaaag taaatagtga atctccaact ttaaacttca
     9781 taccctctac tatagaaaga gttaatggta tttttatagt aaaggttgtc ccttcatcct
     9841 gtttactttt tacttcaatt gaacctccaa ctttttcaat acttttacga acaatgtcca
     9901 tgcctactcc tcttccagaa aattcagtta tagattcttt tgtagaaaaa cctggagtca
     9961 taatcaaaga attgacctcg tcatcagtat actcattaat ttgatgttta agaagacctt
    10021 gttccttagc ttttgccaaa atcttatctc tcataatgcc tctaccatca tctgaaatac
    10081 ttatcacagc ttctccgcct agattctcag cagataaaac tatctttccc acttcttctt
    10141 tacctttcgc tatacgctcc tcaataggct ctattgcatg gtccatagca tttcttataa
    10201 ggtgcataaa aggttctgct aaattatcaa ttacattctt atctacctct gtctctgctc
    10261 ctatagttat taactctaca tttttatcta attttttatt catatctcgt acaattctat
    10321 gcattttttg aaatacacca gcaatttgaa tcatacgcat agacattact atatcttgca
    10381 attcatctgt aagcttgtgt aattgtcttg ttgccttatt gaaattatct atatcaacac
    10441 ctgtagaatt tacgctattt acaaccattg attccataat tacaatttct ccaactatat
    10501 tttgaagtaa atccaatttt tctaaattta cactcataat actctgctta cttattggta
    10561 aatgttcatt ttcttcactt agactatctt tttccatatc agttttttca tttaaaacat
    10621 cttcttgtat ctcatcttct gtatagttat tttgataact tttacttact ttattattat
    10681 cttttaattt attttctact gcatcattat ttgatgtatt tttcatttct atttcttttt
    10741 tactaatatt tatgtattct tttacataga gattatcttc tatagtttta taaactattg
    10801 ccttatcctc tttagctaca aattgtatat aaaacccata acttttaata tattctgtag
    10861 actctgcatt tgtttctaca tcttttggat aaaaatctat aaattcacag taatctctaa
    10921 gttcaaatac aagcatgaac gctctcacat tttccatcat gcaaccttgt tcaaagatta
    10981 tttttactac atcagagtct tctttgtatt caaaatttga atcgattgct tctttttcgt
    11041 ctgtactttc taaatttgca ttatcctttg atttcataga acttgataat tccaaagtac
    11101 tcacatactc ttcaattcct tcaataaaat tagagtaatc agaagatata caacctcttg
    11161 attgtagctc atctatatct cttcttataa aatctaatga ttgaaagact aaatcaaata
    11221 ataggtcatt gtctaaaact tctattttac cttcacgtat agcataaaac aagtcttcta
    11281 cactatgtgc taaagcagat atattgtcta accccatcat agatgctgaa ccttttatcg
    11341 tatgcattat tctaaaagtt tcattaatgt tgttatgagt aaatactttc tctttttcta
    11401 tttgaatcat aatttcatct aattgttcga gtaataaact tgtctcaaat ataaacatat
    11461 ctaacatttg tctcatactc ggttcaatat aattcacatt tatcacctac gacctacaaa
    11521 taattttcta ttatatatgc atctcataat ttaaatcctt gatgattctc ttatctcact
    11581 atatgtacct cataatttaa atacacaatc attctcttat ttcaatatat atacatctca
    11641 taatttaaat acatgataat tctcttactt caatattcac ctcataattt aaatacatga
    11701 taattctctt acttcaatat tcacctcata atttaaaccc atgatgattc tcttacttca
    11761 ctttccttta gatgaaattt ttctactaat tcttttaaaa tttgagcttg agcagacatc
    11821 tcttctgagg ctgctgcact ttcctctgct gttgctgagt tcatttggac tactatggaa
    11881 atttgctcta ttccagataa aatctgtgta attgctactt cttgactatg tacagccgaa
    11941 gaaataccat taatataatt agtagttttt tctacaccat ttactaccaa gtccaatgca
    12001 tttgcagtct ctttagcaat tctgacacca ttttctactg ttttaatagc ttcttctatg
    12061 agaatagctg tattttgagc tgcttttgca cttttaccag ccaaagacct aacttcatct
    12121 gctacaactg caaatcctct accatattga cctgctcttg ctgcttcaac agctgcattt
    12181 aatgcgagaa tattagtttg gaatgctatc tcatctattg ttttgattat ttttgatatt
    12241 tcactcgatt tttcacttat ttcacccatt gatgtcatca tatcatccat acatctactt
    12301 ccaaaaccaa cttgtgatgc agattggtca gatacctcac atgcaagact agcactatct
    12361 gcacacttct taattccatc tgatatctca ttaatcgtgg aagctaattc ttctactgaa
    12421 ctagcttgtt cagtagcacc ctgtgaaaga gcttgtgcac ctgctgcaac ttgttctgaa
    12481 gcacttgcaa cttgttctgc tgataaattt atttcagaaa aatcaccatt aaaagagttt
    12541 attatacttt ctaaagatat tttaattgat ttaaaatcac ctataaattc ttggtctatc
    12601 tctactctta aatctccttt tgcaatacta gttaatgtac tgtctataag gtctatgtaa
    12661 tttaaaagca tactttgcat ctttttgaca gaactcacta atagtcctat ttcagattta
    12721 ctttcttcaa cgtctatact tgttcttaag tccccagatg caattcttga aaagtgtttt
    12781 tctactctat taataggaat cactatcttt aattttatta ctgtatattc tattattgtc
    12841 aatattatta aaagggaaag tataaatatt gttcttaata ttagactttg taattctttt
    12901 tttacttcta caattttgga atttgaacgg gtaactatat cctcttgaaa ttcatttatt
    12961 aaagaatcca ttttctcttg ggagtttata tagtcactac caaaaattat actttgagct
    13021 ttactcttat cattactttc gactgccttt attgcacttt gttctgttgg tactaaagaa
    13081 tctgatattt cttttgcttc taagatttta tttgactcgc tctttgtaag accaattttt
    13141 tctaatttgt ttaaagctga ttctcttgtt tttccgtata attctttttc ataaatatct
    13201 ttattctttt tttctcctgt aaatacatat cttcttaata catcacttat ctctctagtt
    13261 gtatctttta tatactgact ttgagtcctc aaattattca tatcattaat acttttttct
    13321 actttttcaa atgctttata agatgaaata gaagaaaaaa tccctgctag aacagccaat
    13381 actataataa tcagcaatac aattgctaat ttagaaagct ttacttctct tttcatatct
    13441 gtactccttc ataaagtaag tttatatctt ttttaatcct ttcattgtac tcactacgct
    13501 acaatctttt cctaagtttt taccctcata cattgcttta tctgctaagg atatgttttc
    13561 ttctaatgat atatttgagt ttacttctac tactcctaat gtaatagtgc actttatttt
    13621 atgttcttta tagttgaaat caaactcctt tatctcttta cggattcttt ctgaaagtat
    13681 cttagcacct tctattggag tatttgtgag cattatcaaa aactcttctc caccccatct
    13741 tgatacagta tcaatttgtc tacaattctt ttttattata cttgatatta ctgttaacac
    13801 gaaatcccct gcttcatgcc cataagtatc attgataacc ttaaagtcat caatatcacc
    13861 aaggataagg gatattgatg tttcactata tttattaatt cttctttgct ccataagctg
    13921 attaattata tgtcgcctat tgtaaagacc tgtaagataa tctcttacaa cgagtctatt
    13981 caatttttca agagaagatt ccagttcatt atttatcttt tttaatttat cttgagattt
    14041 ctttacttca agatgagcta taacacgagc ttttaattct gtcatactaa aaggtttcac
    14101 catatagtca attgctccta caccaaatcc ttttactatg tctgtatctg tattttttga
    14161 agttataaaa attataggta tatctgctgt acgagtatta gattttactt ccttacaaaa
    14221 ctcaaaacca gagttatctg gtaaaattat atcaagcaaa ataatgtctg gtataaattc
    14281 ttcaataaat tcttttgcct ctattgcatt aaatgctttt ttaaccttaa aattatcttt
    14341 tgtaagtgtt ttatctatta agtcacacat cagaacacta tcatcaacga ctaaaattgt
    14401 tccctgttgc ttatttttca tatacaaacc tctcttaaac ataatatatt caaattttaa
    14461 tgatttaaaa tttttatact taataattat accactatat tcttaaatgt tctcatcaca
    14521 atctttttat aataacactc caactacatc cctatgttac atgatagtgc aatcaaaccc
    14581 attgattaat tcatctatta aaacttcatc tttatctgtc aaaagtttct ctaaatctag
    14641 taaagtaatt attttacctt ctaattctcc aacgccactt acaaaataat ccgaatattc
    14701 tatagtaact cttggtggag gtgatatttt acttttttct aagcttacta tatcatcaac
    14761 tctatcaacc acaaatccaa ctaccaagtc ctttatttca gttactacta tatacatgca
    14821 atttgtagtc attaattcat cttttttaag tctcaaacgc atatctatta ttggaatgat
    14881 ttttcctctt agatttatta tgcctttaga atatgtaggt agttctggaa taaatataat
    14941 ttcttgattt ccagtaattt ctattacctt ttcaagttca accccaaaat cttgctcatc
    15001 tactttaaac tttagatact taattccttc atcaattagt ccataatcta aatatgggtc
    15061 atctggaaat ctcatttaat acctccctcc taaaatacac tttctatgaa atatatatta
    15121 taatttcttc ttacctttat ttattgaatc tacaagtaat tcacccgtct cagtgtaaaa
    15181 acgtattgtt ctaccataat tcatacctgt gtcctcagct aatattggaa tatttaatcc
    15241 taaaagagtt ttttttactg cctcaacatt cctatgtcct atacttgcaa ttactgaatt
    15301 gctgcttcct aaaaacatct gagcacctcc agcaattttt gcttttatat atattctatt
    15361 tgcacctata ttctccatag atttaatgag ctcatttatt cctgtgtcag caaatttaca
    15421 cttattttca atgttaatag catctttact atatggcaac ataacgtgta acattccacc
    15481 aattccagct actttatcta tcaaacatac tcctacacaa gagccaagtg cataagtaac
    15541 taaaacattt ggtctatagg ctatcttcat atctgcaatg ttcactacta tctctctatt
    15601 catacttcta atcccaacct cacgagtatt tctctaagtg agctcatttc tggaatcatc
    15661 aaaatgtggc tagtaaaata attatcactc attttaaatt tattttctat aaataagact
    15721 ttgtcaccaa catctgaaaa tcttatcata ggcacattta gaacagctcc aaccatatca
    15781 atacatatat ctggaattga aacacttata gtcatgttta acatagatgc aatagcattt
    15841 acatatgaac ttgccataat atttcctatt tccttaattg ctgaaatttc aattgcctca
    15901 atctcatcta aaaaagcttc ttctttatta aaaagagaac ttagcataag ttgagttatt
    15961 gattcatcca acaaaaacat aaacattcca ttgatatcac ctttcatatc gagtaaaata
    16021 catactactt tgttttctgg accaccaagc aaatttgtcg cttcattata ttcaagtatc
    16081 ttgaccatag gtattaatac ttctacattg ctatttatca tggatgcaag tgcagtaatt
    16141 gcattcccag agcctatatt accaatttct ctaagagcat ctatatgaac atcatcaagt
    16201 tctaaataat ttataatata aacccctccc ttttattatt gcatttattt gcttttcatt
    16261 gtatcagtgt atatttacac ttttatttta aaacattatt tacaacttta actaatgttt
    16321 ctgttttgaa gggcttcact ataaaatcta acgctccaag ttttatggct tctacaatca
    16381 tattctcttg tcctattgca gagcacatta caaccttagc acttgaatcc aactttttta
    16441 tttctcttag cacctctaaa ccatcttttt ttggcattgt aatatcaagc aacatcaaat
    16501 caggcttttc atatttaaat atattacacg cttcttctcc atcatttgct tctattaaat
    16561 tattatagcc acttttactc agagcttctt taattatcat tctcataaaa atagcatcat
    16621 ctactatcaa aattttattt tccataaaac taactcctcc atatgtacaa caataaacgc
    16681 ttttatcttc agttattgtc tatactatct gactattttt cgctacacaa taaatacatt
    16741 tcattgataa ataaaagtaa ttatttatgc catattatac ttaattctct taacataatt
    16801 tgtcaacaaa aaaacttata tatctattgt aatatatgtt tggcgttttg acgagttatt
    16861 aaaaatataa ttgtataaaa cataaaaagt tatcaaaagt aatcaaatta taataaaaca
    16921 actttatacc ttttttatgt ttgtataaaa aattttttca gattcattaa aatcatttct
    16981 gtagattcat ataggatttg actttctcaa tatctaatag atagttgttt atttacttaa
    17041 ttatatttct ctatatgtat tatcgattaa attagattta gctttataca ttatttttta
    17101 tatttttcaa attgtatact cataaatcca gaagtattct aatgtgttta taatactttt
    17161 tttcatattt taaattaaat aacaaatcat tatatttaca agtatcattt atcaataaac
    17221 aatattaaat gacgaatatg tctgaagatt caaagtgata ctgatactat taattactaa
    17281 aaaagcagta aatcttttac agatttactg cttatagtta taaatagtaa aactatctat
    17341 aataaatgta ctatactcaa tacttcattc aatatagtaa taacaaatct actttattcc
    17401 tatgtaaata tgaatttctt gtttctttgt atcaaaagta ttacattgat actcttcaaa
    17461 atcacttata taagtcctat caaaatccat ttgccaaaac ttaaaccaaa aatctcctac
    17521 ttctttttga cctcctacta taacaaactt tgcatactta cctgctagta taactttact
    17581 aataatactt tctccaacag tattattagt gtttaattct tctaaattct tctctttatc
    17641 agaattacta tttacttcac aacatgcaac aaagctatac ggacttgtaa aatcaccctg
    17701 gtaatctgta tatagaccta ttgtcttgtc attcttttta ccctttatag cattatatat
    17761 ccctttgccc ataaattttt tccacaattc acctatagca tttaccgctt gtccatcttt
    17821 atttgtagtc tccttcgata ttcctataac tattttctca tttatctcta ctatttcata
    17881 attcatactt tatacctcac tttgttttta ttaatatatt ttattttgaa ctaattcaat
    17941 tataatttat aatatacgac acctttatgt catattttta atataattaa actcagtgag
    18001 atattatata ctaaatgcaa gatattatat atttaataaa atctttaatc tagcatcata
    18061 ttataatgaa taaattttag acaactccct tagtctagtt attacattat ccctaacatc
    18121 tttaggttca agtacttcaa cattgtcacc aaacgataaa atatatccat aaatccattc
    18181 accaatagga aattctgtaa ttacatcata tgtatcatct tcatttttag ttattaaatc
    18241 cttattaaaa tcatcaaata ctctaaaaag catcttttct ttaaacctca atttcaacgt
    18301 tatagtattt tccttaatta ctttagaatc attcaaacat acttcttcta ttatctttct
    18361 tgaaaatact tcatccttta tagataaatt ccttatccta ctaattctaa atatcctaaa
    18421 atcatctttt agtttacaaa atccatacaa gtaccaagtc ttactcttat acataagctt
    18481 cagtggttcg acagttctgt ttgtctggct tgacaaacta tttacataat taaaatttat
    18541 agtattacgt cttaaaatag cttcttttat ttttgtaaat ttatcatctt cattaaaatt
    18601 acttccccat tcagtaaagt ctatttcaat ccagtttgaa aaattctgct ctccaaatat
    18661 tgagccaatt ttatttacta ctgaatttat ttctggatac ttagtcgcct gtaatgtttt
    18721 cagtgcaact attaaactct ctttatcttt ttttgacagt atggccttgt ctatagaata
    18781 ttcttctatt aaagatatcc ctcctccttt gcctttcgtc atatatactg gtactcccga
    18841 catagacaga gtttctatat ctctatatat ggttcttgta gaaacttcaa acctatctgc
    18901 caactcttta gctgttacta attttttatt taatagaatt actattattt ctgttaatct
    18961 atttattttc attcattacc acctactaaa attattaaat ttatttaatc taaattaatc
    19021 atactaattt ttaaataaat aaaatcttag aatcttatct aaattattct tacatgattt
    19081 tatttacttt ctattatctt tctcactttg gatattatca tcttaattta tttattacta
    19141 tatcaattta cttattatca tctcatttgt tatcatctta cttaatttac ttatttctat
    19201 atcaattcac ttattatcat ctcatttgtt atcgtcttac ttaatttact tattgctata
    19261 tcaattcact tattatcatt tcatttgtta tcgtcttact taatttactt attgctatat
    19321 caattcactt attatcattt catttgttat catcttactt aatttactta tttctatatc
    19381 aattcactta ttatcatctt aattcatttg cttattatca tttactttat tcaatttcta
    19441 tatcaaaaat tagtatttgt acttaatact taaaattaat ttaaagtctt tacatattat
    19501 ttattttcta ttgtaatatt catttccata ctttactaag ctacttccac tcatttattt
    19561 taaaatttca atttactatt tctcaactct atattattat ttttcaattt tatattacta
    19621 attttaaagc tttttagcta ttattcctac aatatttaaa caattttttc ataataaagc
    19681 tatattacaa tatattcaaa atatgtaata ttttagatat ttttcgttga caatgttaaa
    19741 tttttgtttt atccttaatt aaggacatgt tattgacatt tctgaaactt atttgaaata
    19801 gttttttcat tttatttagg aaaacgtatt atacttaaga aaacgtattt tctcatttca
    19861 gataagtaaa atatttattc ttttggggag gaaatcatgg aaattaataa aaaacatttt
    19921 attcaagtat ttttaacttg cttaatcttg gtagtaatat gtgaatttat agggcaaaga
    19981 actatagcta ttggaaaagc ctctatagta ctgttcccta tgctttatgc agttataatt
    20041 ggacttataa taacaccaga tttattaggt aaaaaaatta aagcacttaa aaaagtggtt
    20101 ggagaaaaag aaataaatat tgctggtgaa gttgttggaa ttgcactagt aatgcttggt
    20161 ataaaatatg gtactacagt tggaccaaac attgacaaaa taattcaagc tggaccagca
    20221 tttatcgctc aagaatttgg tcatatactt gcaccaatcg ttgcattacc tcttgcatta
    20281 atgtttggaa tgaaaagaga agctgttgga gctactgcaa gtataagtcg tgaaccttct
    20341 ttaggagtta tatcagaaaa atatgggata aactcaccag aaggtagtgg tgtacttggt
    20401 acatatttaa tgggaacagt tcttggtact atatacttta gtgtactagg ttcaatatct
    20461 atttatagtg gtcttcaccc atatgctttg ggaatggcct gtggtgttgg tagtggaagt
    20521 atgatgacag cagctgctgg ttcattatct accatggtac cacctgaaat ggcagataca
    20581 atacttgcct atgctgcaac aagcaatatg atgtctagta taacaggaat tatattctta
    20641 gtttttgtat ccttaccatt tactaatttt atgtataaga ttttagaacc taaattttcg
    20701 agaaataaaa aaactaaaga aattaaatct acagaaggag agtgctaata aatgtttgct
    20761 aatttaaatt taaagaaaag ttcatatcaa ttattggtta tgtctgttat tggagttata
    20821 attcttatag gacaaagaat tagcgttggt acatctatag ttacagctct tcctggtatg
    20881 gtgatgctaa tattagctgc tatggcagct atgattataa aagatttatt tcctaaatca
    20941 atctttccag cttttggatt tgcaactata atcggccttt tacttagtat gccatatagt
    21001 ccaacatctg aagtattctt aactaacact aataatatta actttatggc tataactact
    21061 ccactactag cttttgcagg aatttctgtt ggaaacaaaa tagaagcttt aaaagaaatg
    21121 tcatggaaaa tagttataat ttcacttatt gtatttacaa ctatattctt tgcatgtgcg
    21181 agtatagctc atatagtttt aagtattcaa ggtaaaatat aggaggaatt tattatgaat
    21241 aaagaacaat taaaatctct agtaataaaa actatagatg aaaatagaga aaaaatatta
    21301 aaaataggtc aaggaatata caaaaatcca gaatatggat ataaagaatt taaaactaca
    21361 gaagctgttt caaacttttt tgagcaagaa ttaaatttat ctgtagaaaa aaatatagca
    21421 gttacaggat gcaaagcaga tgcaaattct agcaagaaag gtcctcatat atctatactt
    21481 ggagaattgg atggtatatc atgtaaagaa cataaagatg ctaatgaaat aggtgcttct
    21541 catacttgtg gacataatat tcaaatagct ggaatgttag gtgctgctgt tggtttagta
    21601 aaaagtggcg ttttagaaca tttagatggt aaagtatctt ttatggctac tcctgctgaa
    21661 gagtttattg agttggaata cagagagcaa ttaaaaaaag aaggaaaaat aacttatttt
    21721 ggtggaaaac aagaattaat aaaaagaggt tattttgatg atatagacat atctatgatg
    21781 tttcatgctt ctgatttagg agaagataag gctctagttg gtcctgagag taatggattt
    21841 gttggaaaaa aggttaaatt tataggtaaa gaatctcatg ctggttctgc tccacatgat
    21901 ggaataaatg ctttaaatgc agcaatgctt gctataaaca acgtgaatgc acttagagaa
    21961 acttttaaag agtctgaaag agttagattc caccctatca tcacaaaagg tggagatata
    22021 gtaaatgttg ttcctgctga tgttcatatg gaatcatatg ttagagctag aactataaat
    22081 ggtatgattg atgctaatga aagaataaat aaggctttga tggctggtgg aatggcagtt
    22141 ggagctgatg ttgagataac tgaaattcct ggatatcttc caatattacg atacaaaagt
    22201 cttgataatt tatttaaaaa caatcttgaa gatttaggtc taaaaggaaa agttattgat
    22261 ggtggagatt ttacaggctc ttttgatttt ggagatgtgt ctcatataat gcctactctt
    22321 caccctatga ttggtggtgt aaaaggagct cttcatacaa gagattttga aatagttgat
    22381 gaagatttag cttacataat tcctgctaaa gcaatggcac ttacagttgt agaccttctt
    22441 tttgataatg ctaaggatgc taaagatata ttagatacct ttactccagt tatgactaaa
    22501 gaagaatatc taacattcct agaaaaacac gataaaactt tataagtcta tttttataat
    22561 agggtggtat ctactgtaaa agttatacca ctctttgttt ttgtaacagt atacttcttt
    22621 tcttttagct tttagattaa actaaaatat attatcctcc ttaaaacttt tagagatacg
    22681 ttaaaatatc agtagaacta tgctatattc tttctaatta atataaattt atattctaat
    22741 tcaaggtggt gaaaaaatgt ttaatgcagg aaatcgaatt ataaatacat ggttgtaccc
    22801 tattcaagat ggctttgtct tgattgatac tggatatgaa aatggtttta aacatttcaa
    22861 aaaacgaatt tctaaattca acataaaaat caaagatatt cgatacatat ttctaactca
    22921 tgctcatgat gaccatgttg gttttctaaa tcagattcta gacgaatctc ctaatacaaa
    22981 gattgttatg catgataaag ctttagaaat tctatataaa ggtcaaaact cttttattgg
    23041 tggttgtaac agccgtactg cctttctctt ttgccaaatt atgaaactat ttggaaaagg
    23101 tgagcatagt ttttctccac ttaaacaaga atttgaaaaa aggtgtattc ttgtgtctga
    23161 caagaatcgt aaagaaattg aaaaagaact aaatggaaaa attatagata caccaggaca
    23221 tactgctgat tcaatttcac tactaattga tgatggtgtt ttattttgtg gagattctgc
    23281 aatgaatgga tttccaagta ctcacaaaat taccatattt gcagaaagca aactagaatt
    23341 tattcaatct tggaaaacaa tcatcaatac aaaacctaaa atgatttatc ctggacatgg
    23401 aaaaccattt gaatacagta aattagaaca aaatctaatc tatgtaaata aaataaattt
    23461 atatcctcta cataattcaa aagatattca cagatttttg tagaaaatac aaagtaaatt
    23521 tctttgatta attaaaaaat ctaacacttt actacgctac ttattttaat aacttaatct
    23581 tccactagct tttcataaaa ttttgtttta aaattaactt taaagtgcat atgctattta
    23641 aatacattag atataattcg tcattaatct acttaaagca tatttaacta acaccactca
    23701 accagaaaat tcagaaaact gcattgacac aggaaaaagt ttattttata ctttttttag
    23761 gacatattgt ggacacgtct taaatcgaca agcagaattt atattgtaag atcaaccaca
    23821 atacttattt tttaattata tttttaaaca ttagggagga tattatgaat gataacgtag
    23881 ttaagaagaa aaactttttt gacaggacat taaacagaat agaaactgtt ggtaataaac
    23941 taccagaccc agttacaata tttttagggt tatgtgtact tttacttata ctatctagtc
    24001 tagttggttc tatggggata tcagtagttc atcctggtac tggagaaact ataactgcag
    24061 taaatttatt gactgtagaa cagcttcaaa ttcttttagg aaacatagtt agcaatttcc
    24121 aagggtttgc tcctttgggt ctagttctag taactatgat aggtgctggt gtatgtgaca
    24181 aaacaggact tatgacagca acaatcaaag catcagtaag caagattcct gaaacaagag
    24241 taactcttgt tgtaatgact ataggtatgc ttgccaatat agcttcagat gcaggtacaa
    24301 tattatttcc accattagct gcacttgtat atcttggtgt tggtagacat ccacttatag
    24361 gtttattttc tggatatgca gcagtctgtc taggatttgc cgcaaacatt atgataagtg
    24421 tcaatgatat acttgctgca agttttacag ttccagcagc tcaaatgatt gattctggat
    24481 ttcaagccaa tgcaacaatg aacttatttt ttatgatagc atcaactttt gttctaatac
    24541 ttttggcaac atgggtaaca gaaaaaattg tagctcctag atttggtaaa tatgaaggaa
    24601 atgcagaact agatgttgat ggaagtctta gtgatgtaga aaagaaaggg cttaaaaaag
    24661 ctggtataag tatattaata tatgtagcta ttatagtagc tcttagtgta atcggtaaaa
    24721 aaccattctt agctgaccca gaaacaggag atttactatc aaacaatgct cctcttatga
    24781 aaggtatggt acctataata gcactagcat ttttcatacc tggtgttgta tacggaaaaa
    24841 ctataggtaa aataaagaag gataaggatg tagtctctat aatggctagt gcaatgagtg
    24901 atatgggtgg atatataata ctagcatttg ctgcttcaca atttttgcaa ttattcacaa
    24961 atagtaattt aggtctgata ctagctgtaa agggcggaga atttttaaaa tcagcaggaa
    25021 taaatggacc tttattgttg ataggattta ttatattatc atgttttata aatttattta
    25081 taggtagtgc atctgcaaag tgggctattc ttgcacctat atttgtacca atgtttatga
    25141 tggttggata caatccagca cttactcaga tggcttatcg tataggtgat tgtataacaa
    25201 atccacttag cccattattt ccgtacttcc ctattatact agcattcaca agaaaatacg
    25261 ataaagatgc tggtatgggt actgtaattg ctaatatgat accatactct atatcattct
    25321 taatagtgtg gactatacta ttaatattgt ttatggtatt taatattcca ctaggacctg
    25381 gaatacttcc ttcatatagc atgtaattta tttaaatctt tctaaaagga gaaataattt
    25441 atgttagata aaaatagact tataaataat tttatggata tggttagaat tgatagccca
    25501 tctaatcaag aattagaaat gtctaagtgg ttggtaaact acttaaaaga aagaaatata
    25561 gatgccatta tagatgatgc aggtgaaaaa tatggtggta atacaggtaa tgtcattgcc
    25621 tatataaaag gtgaagaagg aagtagacca ctttgtttat gtgcacacat ggaccaagtt
    25681 caaccttgtt taggtgtaaa accaatacta gatggtaatg tagttagaag tgatggaact
    25741 actacacttg gtgcagatga taaagctgga atagctgcta tacttgaagc tttagaacat
    25801 gttactacag aaaaaatacc acatagagat atttatcttt gttttacaat ctgtgaggaa
    25861 gctggtatgc atggtgtcaa aaactttaat cctgacaact tgccttgtaa agatatggtg
    25921 attttagatt ctggtggagc tattggttcg atagcataca aagctcctgc tcaacaatct
    25981 ataaaaatct cttttcatgg aaagaaagca catgctggta tagaacctga aaaagggtta
    26041 aatgctatat tagtagcctc tcatgcaata agcaatatgc acataggcag aatagatcca
    26101 ttgactactt caaatatagg taaaatagaa ggtggtggag ctacaaacat agtaactgat
    26161 aaggttacat tgactgctga aattaggtct catatacctg aaactttaga gtatgagctt
    26221 aatcatatgg aaaaatgttg taaggatgct gcatctaaat ttaatactac ttataccttt
    26281 gaacatagta tgtcttaccc tagttttgaa cttagtagag atagtcacgt tttcaagtta
    26341 agtgaagaag caataagaca agttggtgtt gttccaaatc caatggtaat tggtggtgga
    26401 agtgacgcaa atatattagc taatcttgga tataattgtg ctatattaag tctaggtatg
    26461 tatgatgtac acacagttaa tgaatatgta aatatagacg aattatatga tactgccaaa
    26521 atagtttatc atatgattaa attataaata tcataatata cctctttaaa ttataaaaat
    26581 aaaaacctat gtatcttcaa ctttataagt tatatattaa agttgtttta cataggtttt
    26641 agtatcttat ataataattt attttaacat ttttcataat aagctttggt attttatata
    26701 gtaatttgtt ctaacatttt tcataataag cttcaatatt tttcataata atttgttcta
    26761 gcatttttaa taacaagttc tagtattttt ataataattt gttctagtat ttttaataac
    26821 aagctttagt attttacata ataatttgtt ctagtatttt taataacaag ctctagtatt
    26881 ttatataata atttgttcta atttttttca tgataagctt taatacttta tatagtaatt
    26941 tgttgtagaa ttttataaaa tataatgatt tatacagttt tataaaatat cttaaaacag
    27001 tatttcagta atattttttg gtcttcctcc accaatacta gtctcttttg cacatattcg
    27061 acctaaacct gcatttacta ttttatttaa tatacgtctt gcacttcttt cacttacatt
    27121 tagatactct gctaactctt ttgaatcata aatcttactt tttctattct cacttatagc
    27181 aataattttt gcaactgact cacaactcat tccagtcttt ttagatattt ctattacctt
    27241 ttcatcagat agcatcaaag aataatttaa ttcattttcg gaagctaaag gtcctcttat
    27301 attttcatct tcatctacca aatatatacc aatttctttt gaatctatgg agtgtcttaa
    27361 tgctttatgt gcattatttt cagcctgata cgcagttact ccagtaccaa ttcctatact
    27421 aagagaaaag cccatgtctt ttatctctct ctttagattg aatagcttat cataattata
    27481 ctcattatca gctacccctt tatgaacaaa tacaacataa tcatttcttc caagtaaaaa
    27541 taaggaacct tgaatgcttc ttacatagtt tactacaagc ttatccatat ctgatttttt
    27601 tatcatgtca gaataatagt tatctatctt ctctttataa tctattagac tcaatatttc
    27661 gacagcaatt tgagaaaatc ttgccttgct tagagcatat tcactcttta ccttatcata
    27721 actttctttg attaattgta tagtagcttg taaacgaaat acaggatagc ctctttcttt
    27781 taattcatta tatactgcac caaaaccagt taacattata tccgtcttat tttcttcaaa
    27841 taattttata tgccattcta caagttcaga ctctcctact tccaaagaaa atggattaca
    27901 aaatacactc gttgctttaa tatcaaattc tttaagtgca tcttccacaa catatctatc
    27961 aactacatct atactgaatt tatctaattt tatatttgca ctttttattg cccaaaatgc
    28021 cttcattata ctcgtcccac ttctcggtac aaatgaatgt ggcttactta tctcatactt
    28081 agattttata aattcataaa ctgcacatcc tgtaaatata atagcatcac attcatcttc
    28141 acatttagaa ataacttcta ttgtatctac tactttttct cttacatata ttttagtctt
    28201 taaatcactg tctatctgtt ttaaatcatc ctttatctta aacccagagt ctgaaggacc
    28261 tataataccc actatcataa acttcgctcc taatccataa atattatact ttttaaaaat
    28321 tatatcctca ctattttcta cttctattta attatgttga actactccat cctacctaca
    28381 tttagagtgg gataattagc ttatttttga taaagtaact tctcaaaatc agcacttgga
    28441 ataggcttac aataataata accttgaatt accacacaat ctaaactcac taaaaattca
    28501 gcttgttctt ttgtctctac accttctgcc actacatcca tatctaaatc ttttgccatg
    28561 gctatagtgt gccttaaaac tattttttct ctgtctgtga ttccttcctc attaaagaag
    28621 ccacaatcta tcttcaatgt atcaatatta atatttttta acatatttaa tgaagaatat
    28681 gctgaaccaa agtcatccat atttaaaaca ataccatcca ttctaaggtc ggctattatc
    28741 ttagtaaata attttatatt tttaataaat aaactttctg taagctccaa ttctaaaaaa
    28801 gattttggta tactatattt atttattaaa cttaaaacaa tttctttaaa atgtggatta
    28861 tgcaaatgta atcttgatac atttacagag ataggaactg gctcttcacc taaatctatc
    28921 cattttctaa tcaaagaaca tacttgttcc cacacataca tgtctaatct gataataaac
    28981 ccattcttct caaatagtga aataaattta attggaggta ttataccttt ttctggatga
    29041 acccatctaa ccaatgcttc cgcaccaaca attttatttg cattaagatt atacttaggt
    29101 tgtagatata catggaattg cccattttca agtgcgtatt ccatttccct ttccatctgt
    29161 ttttcttcaa gtgcatcatt tcttatagta tcatcataaa atatatagtt attgatactt
    29221 tgtttcttta cttttttgtg agcatatcct gccatttcac acgcatttgt aattggaata
    29281 ttattatcca aaattttata aaccccaaaa catggtacta acatatattc aaattttttt
    29341 ctatttaagc tatttttaat cttataaatt aatttatgaa catctctatc agagtaactc
    29401 tcataaaaaa tacaaaatac atctgcatac atacgacaac atataccatt agaaccaatg
    29461 tttttttgaa tcaattctgc aacatatatt aaagccttat ctcctcctct aactccataa
    29521 atgtcattaa ttaccttgaa cttatctata tccatcatta taattgcata gctcttgtca
    29581 ggatatcttt taagcattaa acttgtttgt tcacaaaaac tttcataatt gtatagtcct
    29641 gttaatatat ccttttggtt tttatgtagt tgcataagat aatctatatt ttttatagca
    29701 actaaaatat gtgtttgttt ctcacctaac ttatttttac tactaatctt taacatataa
    29761 attaatgccc aatagtactt tttatctatt cctaaacgtc tatactcaaa atttagttct
    29821 aaatcgtctt gcttgctgtt attaagacta tcaagagatt ttagaaggtg gttatcatca
    29881 aataatatta tatcatcagg atgaattaaa tgttttttta tgtatgaaaa agttttttca
    29941 agattttcct ttataggaaa actaaaaata ttgctctctg attgataaat tggagtaatt
    30001 aaattagtat cgatgcaaat agataaaatt atatcatatg tattataagc taatttttca
    30061 taagccttag aatacccttt tatcattaaa tcatcaaaat aaaccttatc aactaactct
    30121 ttatgtttaa ataaaatatt attcgtcgaa tcagctaagt cactaattac ttccatttca
    30181 ttatttctaa gtttgttctt attataatac ctcttcttat cttggtacat tctctcatca
    30241 gcaactttaa ttagcttatg tatatcaata ttatcgtcac tccaaatact gcccactgct
    30301 acactatata tcatctcatc cttaaattct tcacttaacc aactcactaa ttcttcaaat
    30361 agttgttttg atatatcttc acataataca ataaactcat ctcctccaat acggtaaaca
    30421 ttataagaat gaaagtattt ttttattatt acacatgctg acacaatagc agagtcgcca
    30481 ttagcatgtc caaagttatc attaatatct tttaacccat ttatatctac aaatacaact
    30541 cctatggatt taaattttcc tttttgaaaa ttatctatat acttaatata tttgtttcta
    30601 ttatatgcac cagtcaagaa gtcatagtag ctcatataat ttaatttatt ttctactttt
    30661 ctttttttca tctcagttaa taaaaaataa gatagggatg tcaaaagaga ggtatctcca
    30721 acagccttat gtggattatc aactccaata aaccctacta ctttatcatc ttgttttagt
    30781 gaagatgtca ttaaactttc aattttttga ggttttaaaa tttcatattc tattggctgt
    30841 tcttctttta tattttctat atcatcaatt attacaaaac ccttttgttc gaataataaa
    30901 aaccaattct ttaattcatt aatatcaaga tttgttagtg cctgtttttg ttcatcaaca
    30961 ccttcattgc accattcata tgtattagtt actttttttc tatcttctga tacttcaaaa
    31021 acatatgctc tatcagcttt atgaaattta ccaatattgg ttaatacaag atttacagca
    31081 tcagaaaaat tagattctgt tataagatgt ttaatacatc caactaataa ctcctttatc
    31141 tctaattttt ctgctattga cttacttata tcttcctttt ctgtaatatc aagagctatt
    31201 tctaatcgtg catgtttgcc ctcccattta attaacttgt cctttaatat aaaatgtcta
    31261 tttgttcgag tattcgtatt ttcccatatg taatattcat cttcttttaa aatagagttt
    31321 gtacaaaatt cgcagggaga atctttcccc tgtaatgctt tataacattt tacatgggat
    31381 atatcattaa ttcccattgc ctttttccca atttcattaa tatataaaag ttcatatgta
    31441 tctaaagaag aaacatatac aatttcatcc aactcatcta ttaaatttat tacatccatt
    31501 tatatatatc tccttttatt cattttccag attgaatttt taaagctttt aaaattttgc
    31561 tatatatttg gcaatgatat tatacttctt tatgttttaa aatagtacct taataaagtt
    31621 ctatttttta gtaaatagaa ttattattgt gtcaatcgta caatagagtt ttgtaatacc
    31681 taatttaata tatttataat taactatctt attatgtgtt tacaattaat tctcatataa
    31741 atgacaaatc tccacctcaa ttaagtattc caatcatata tgaagataat acttcattca
    31801 tttaaaaata taaaactatt atatattaaa atttcttcaa taataaatta tctagattgt
    31861 tactttgata tattatataa tatcacaaag gctgatataa aaagacaaaa agggtaattt
    31921 ttttatatca aaaaaaatgt taaattgata ttatccatat ataaattgat attatccata
    31981 tagatgctat atttaacaat tctacttact tcccttacac ttattagcat atagttatta
    32041 agagtttgtt gcaaaacaac acttagcaca ctttaaaaag tgtggtctaa taagtaatca
    32101 acgaagaaga cataataaag cataaaatat gatatacttt tcctgtcatc aaaactgatg
    32161 attttactat taatatctgc taaaatatgc ttaataaatt aaattttagt gggagaatag
    32221 ttatgaaaat atttagtttc ttgttgtttg tagtgggaat atgtttttgt attgctggat
    32281 ttatgggaca ttacccagca tttgctggtg caacattgag tttttttgca actacaatga
    32341 tagctgtaag acaagtcaaa actaagaaaa aatgaaatag gcaaatccta actttacaag
    32401 ttaatattac ataagtaatt agtctaaaat tggatacttt ttctattaca gaaatacatc
    32461 atccactttg tattctctta aaaaaataaa atagctatgt aaattcaaga ttagatagat
    32521 taactctcta tccatttcat tgtaaaatat acatagctat ttgtattcga tttaaaatac
    32581 ttagattttc ttacataaca aataatttct atctgattat tactcatatt catttagtaa
    32641 tcactcagca actaatcatt tagtatctat tcatccgcca tacggtaacc taccccaacc
    32701 tcagtcaaaa tatattctgg ctgagcagga ttcttttcaa ttttccttct tatacttgcc
    32761 ataaaaactc taagcgactg attttcactt tcatagtaat ttccccaaac tttatctatt
    32821 ataaacttat gagttaagac tcttcctgaa tacttagaca tcaactcaat aatcttatat
    32881 tcgattggag ttaaatgtat ttcttcacca ttaattgaca caatatgatt ttcataatct
    32941 atctttaggt ttttaacttc aaaagacttt ggagcatcat tatttatatt atcctgttga
    33001 gctttatttc taagagccac acgtactcta gccaaaagct ctgttacact aaaaggttta
    33061 gttaaataat cgtctgctcc tccatctaaa gcttccacct tatccctctc atgctctctc
    33121 gctgaaacaa caattatttt attagtcttt gcacatgccc taactcgtcc tattacctca
    33181 atcccatcaa tatctggtag tcctaaatcc aaaattatca aatctgggtc aagagaatgt
    33241 ataagtgata tagctgttcc tccattatct gtctctacac acctataatt ttgagtttca
    33301 agagatactt ttataaattt acaaattggc ttatcatctt ctacaattag aactaatggc
    33361 tttatttcca cttgtctttc ccctcctcat catacatagg tatgctaaat ctaaatgtag
    33421 ctcctccaga aggattatta aatgcgacaa tttctccacc atgagcttct actatagatt
    33481 tacatatctc caatccaaga ccaatacctc ttctttcaag atttctagac ttattcttag
    33541 tataaaacct attaaaaata ttaccgatat cttcatttgg tattcctttt ccattatctg
    33601 caacttcaaa tagcacttga tttgactctt tttttacagt tagttctatc ttagaatttt
    33661 taggtgtatg ccttatagca ttatcaatta aatttactaa cacttgttct attaataata
    33721 catccacatg aacgattatt atctcatctg gaatattggt ttttatgtct ctactattgg
    33781 caaacttttt gactcttaat atagattcag ctattatttc atcaacaact tctggtcgtt
    33841 tttcaatatc caatttccct tcatctattc tagtcatact aagtatattc tcaacagaat
    33901 gagttagcca actcgtatct tcatatatat ttttcaataa ttctactcta gtttctttat
    33961 ctatcacatc atcattttca agtattgttg aagacgaacc caatatactt gtaagtggag
    34021 ttcttaaatc atgagaaatt gacctaagca aatttcctct taatctctct ctctctgcct
    34081 caagatttgc ttttttattt ttctcaaata aattttctct atcaattgcc aacgcaacct
    34141 gtgttgaaac tgcttctaat agtatctttt cattttctgt aataatatct atcttaaaac
    34201 acgcaacacc tataacacca agaacatttg attcttgacc ttttataggg aaataatatg
    34261 cactgctgtt tttgcaatat tttgtcccaa ttccaactgg gttacctgtc ctaaaagctt
    34321 cttttatagc ttctttttct aatactgaat ggaatatatt ctcacttcca tcattgttac
    34381 aaatataaat acttggctta cctagattat tattctcatc tgcaatagtc actattacag
    34441 ttctatttaa tatatttact aaattagacc cacaaaattc aataatttga tttttatttt
    34501 ttgctttaag caacgttttg ttattctcat agagcaatct tatcattttt tctctcttat
    34561 gagaaagtct cacttctctt tttatcctag aagttaaagt acttgttata attgctgcca
    34621 ttagcataat acaaaaagtc ataggataat cttttctata agctaaaaaa gtatataatg
    34681 gatgcgtaaa gaaaaaatta aatgatagca ctcctattac tgaagaaagt attccaaaaa
    34741 aatatccata ggttatactt gtagcaaata gtacacctaa tatgtatact agtataatat
    34801 tagattcatt aaaacctacc ttttcaaaaa ctactgatat tccagtagca attgtcataa
    34861 ccaatatagt ttttatgaag tccttaataa ttatcttttt actgagatag ccatcttcat
    34921 cataatcata ctcttttaag tcttcatgtt gtagtcgttc ctttttcatt tactacaatt
    34981 cctccttatt gttactgttt aaattggctc tattttatat aatacaattt tatcataata
    35041 aattcaatca atacatttaa ttaatgtact taaattacta aaaggtgctg tcttaaaatg
    35101 aaaataaatc tctatttcaa aacagcacct atatatttaa attactaatt tagtttttac
    35161 atacttaaat caatagtttt attcaatttt ataaattata atacttcaat tatggaacat
    35221 ttctatccaa caagcataaa tataaagtgg gcacatatac ctgcacacaa accacctatg
    35281 gtatgcgaaa gtatcgcttt accagccaat ggtcttgcat ctaaagcatc catcatacct
    35341 atgtgtgtac ttaaatatcc actccaacac atacccattg ctgtgaatac tgctatatca
    35401 ttaggagtta tagcgcctga ttttataaac tctggtacta aagatattgc tgctccaaca
    35461 gctcctaatg cagttacagg gaatgctatt gcctctggtg atgtgaatcc aaataatggt
    35521 tctattatga agctaatctt atcaccaata gcaggtaata attttatacc ttcatatgca
    35581 gctcctgtgt aaacagcttg acctgtagca gggtctgtag atggtccaaa agttaataac
    35641 ataactaatg tacaaactac taaaactcct ggtattatag ccatacccat ttcaacacct
    35701 agcttaccac cttcaagtat agcatccaat gttctttgga atacattacc atctcttatt
    35761 tctctaaact cttcaagttt tgcaccttta tcatttattt gcttttcagt atcattttta
    35821 gggtcataat tataatactt tttagtaaaa gtcaacataa ttctaacact aataatacta
    35881 cctattattg ccccaacatt acctattata gctggtagta catattcttt accttgagct
    35941 atcataaaag ttgttacaat aagtcccata ccaaaggctg ttcctaaatt acataaagca
    36001 ggtacttgat acttcttaaa atattgtgta aatgttttgt ctttagcaaa tggtataata
    36061 gctggattat cagataaata tgttgcaact actcctgcta tactagcacc aggtaagccc
    36121 catataggtc tcataagacc cttaaatatc ttattaacta aagatattac tccaaactct
    36181 gaaagcaacg cactaagtgc acctgcaaga actgccatag ccattattaa aaatactgtg
    36241 tctaataaaa gcgcatgtgc tgtactcatt attactttaa acatcattcc tgcacccatg
    36301 atgcttccta catatccaaa tcctaccgcc aatagtacaa taaatacgaa ggtttccata
    36361 cttactgcct tgactcttct cttatccgac agcttttcca tcttgaaatc ttcccccttt
    36421 taattttaaa ttaaggttcc taacattggt acaaattttt tggtacaaac tgagttctaa
    36481 aacttacact cgtaatgcaa gttgtcagtt ttttgaaata aaaaaaaatt acgtttatag
    36541 aataagaata tcctctttaa tttggatact cttattctat tttatgttat tatattatca
    36601 tccttttgtt taaaagtcaa ttttttctat ttttgttgtt taattttatt tatactcttt
    36661 ttatattttt cattttcttt tatctttgta agcactccat ataagttgac ttttctaaat
    36721 acagtagtta taaataccaa tcctaatgca attgaacttg cacatgataa tgtactcacc
    36781 ccatattcta cagcttttat ataattattg gtcaaaacag aatacatagt attatatatt
    36841 ccataaccag gtactaatat tattaaacaa caaacactta atgtagttgc tggagtcata
    36901 taaattcttg cacataactc acaatatatc gcaaaacaaa ctgtagctat aaaaaaacat
    36961 aagttttctg ttactcccat atttaaaaga aatttatatg aaaaccaagc tatacttcca
    37021 cctattcctg ctagtattaa gttttttcct ttcatattaa atagcactcc aaagcttaag
    37081 gcagtaaaaa aagctgctat tacttctata aacaaatgca cttatatccc ccctaacttg
    37141 aagtataatc caagtaccaa gaacattcct actgctatag atacacctac aaaaaatact
    37201 tctgctaatt ttgcaagacc agaaatcaac tgtccatcta acaaatctct taatgcatta
    37261 gtcaaagcaa gcccgggaac taagagcatt attgctcctg ctattaaatg gtctaacgtc
    37321 tttataaatc cgagctttaa aattaaaatt gaacaagaag ctataataac tgctccaatt
    37381 gcatttacaa aaaagtcatt aagtgaagat ttacttaaat acataataac aaacttagtc
    37441 agaatcccaa cagtaaatgc agctataaat tcttccacat ttcctccaaa taatatcgaa
    37501 aaagttgctg ctgcaatacc tgcaaaaaat attgttatta aatccgagta atagttatct
    37561 tcacttattt catctaaaat tttctcacat aattcaatat ctaaattatt tatacttatt
    37621 gctcttgata atgagttaac ttcatgaacc aagtttaggt ctactcttct tgagcttatt
    37681 cttttaattg ctgaatgtaa tttcccatct acaaatacag atgccattac agctgttgga
    37741 gttgcaaata catctacgtg gtctattcca aaagattgac atattctagc tatcgtttct
    37801 tcaactctat aggtttctcc tccactttgc aacatagctt tacctgcatt tgaggaaaag
    37861 tttaaaattc tatctatatc cacaaaacat atctcccatt cttttatagt attattcata
    37921 ttttaataca agatattatt tatttaaact attttaaata attcatatcc aatatttaca
    37981 ttttatgaaa aaacttgttt tttatgcttg tcaaaatcaa attttaagct tgtttacacc
    38041 ataaaatggc tgaattaata tttgacaaac actttcaacc atttatataa ccttctaaat
    38101 tgtataactt tttactacaa ataaaattat atctctttta attctttctc ttgagtattt
    38161 aagcttatag caacttcata tttatatatg tacaatataa aacattattt ttatgataca
    38221 aaatataatt tgtaaacgta tatatgtatt aactgtgtat atataaatta ttcttaacct
    38281 tatcagtttt ttcatatatc ttcatctata tcgaccatat attaattaca ttgaccatat
    38341 actaataaat gatattacat agactgaagt atattaaaaa acttaaattt agttatttaa
    38401 caacataata ctctaattta atctataaaa atccatacct aatataatgc aactcttatt
    38461 ttaacaccat ctattacaca aaacaacata ttaaaaagta aaatacaaaa tatccactat
    38521 aaaccttatt ttaatactga atatatatta taatatattt aatgtagctt aaatatagtt
    38581 taaacataaa gaaaggaaat ggtatatgct aagaaaagat aaagttctat tatctctaca
    38641 taaattatgt gaaaatatga ctttaaaaga ccttcaatca gataaaacag gattcactac
    38701 aatagaaatt gctaaccatt catctcttga tagaagtaat accagtaaag aattaaataa
    38761 cctatataat gaaaaaaaag ttctgaaaat atctggtaag ccaattttat ttctacatgt
    38821 tagtattata gaaaatttaa ttggccgtac tttgaacagt gatgaatttt ctataaataa
    38881 ttgtaatgag ctacttaaat caagtaaaaa tgactcagaa gatatttttt caacattact
    38941 tggacatgat tctagtctta aactacctat agaaaaagct aaatctgcta tactctatcc
    39001 tccaaaaggt cttaatacgt tactcatagg acctactggt gttggaaaaa gtacatttgc
    39061 cgaaacaatg tacaaatatg ctgtagaaag taaaatcttt tcttctgaaa gcaaattcgt
    39121 agtatttaac tgtgctgaat atgcagaaaa tgctaactta ttattaagta atttatttgg
    39181 ttatgtcaaa ggttctttta ctggtgctaa taaggataaa ttcggcatag ttgcacaggc
    39241 tgatggaggc atattattct tagatgaaat acacagatta ccaccagaag gtcaagagat
    39301 gctttttctt ttgatggaca aaggcatata cagacgatta ggagaatctg acattgtgca
    39361 caaagctaat gtctttattg tgtgtgccac aacagaagat atacattctt ctctgcttgg
    39421 gactttttta agaagaatcc ctgttacaat caacctacct gctttacagg ataggtcttt
    39481 aaaagaaaga ttatctctta ttaaatactt ctttattata gaatcaaaat acaccaatgc
    39541 aactataaaa gttcacaaag atgtaattaa atcattactc ctttatgatt gttttggtaa
    39601 tataggtcag cttaaatctg acatacaact tatgagtgca agagcctttt taaattacaa
    39661 aactggactt aaggataata tagaaataga tttatccctt gttcctgatt acatctatat
    39721 tggtctttta aaattgaatg aaaatcgtag taatctaaat aaattatcag aacttgatta
    39781 tatgcttttt tatgaattta atggtaataa cttagaagtt gaatatacaa aagaactatc
    39841 taaatacaat atagatacat caaacaatgg atttaaatac tctacagaga ttccttatct
    39901 ggaaaatgat ataaggagta tgtttataaa ttacatggaa aagtactcta caaaattact
    39961 ttcgatatct tcagtaactc attctgatta tacaagtaat gaaattttta aagtaatcaa
    40021 tccaaagatt tactatgctg ttgaacattc tttaaaagag gccgaaaaaa tatcgagaaa
    40081 caaatacaca aaacaaactt atattgctct ttcaatgcat ataagtgcac ttatagaaaa
    40141 tataaatcaa gacacctata atcctaagta caatttatat aatgataaaa ttttcttaga
    40201 taaagattta aatatatcta aaatagtttt aagtataatt gaaaatgact tacaaacatc
    40261 tttacctaaa gatgaagtaa aatttatatc catgtttcta aatagcatgt atcttgaacc
    40321 aaacaatcaa tctgaaagta taggagtcat aatacttgct catggaaatt caacagcaag
    40381 cagtatgtgt gatgtagcaa attcattgct tggtacaaac cattgtaatg caattgacat
    40441 gcctcttgat gtaaaagtaa gtaccatact tgataaaact atagagttgg taaaagaatg
    40501 tgacgaaggt aagggtgtac ttcttttaac agatatgggc tctcttatag cttttggaga
    40561 acttatttca gaaaaaacaa aaatcaaaac tcgctcaatt gaaatggtat ctactccaat
    40621 agttttagaa gcagtaagaa aaagtacatt accagaaatg actttggatg aattagctaa
    40681 ttccttaagc tctttaaatc catatattgg acgagcagca atcgattcaa cagaaataaa
    40741 ctatcctgaa aagtcaagag agtatgtaat aataactact tgtataacag gatatggttc
    40801 agctatgaaa gtagcagact ttataaattc ttcacttaaa aatattcgta aatacaatat
    40861 taatttagtt cctcataata tagaaagttt taaaatgtat aagaataaat ttagtaatac
    40921 taaaattctg gcagttgttg gaagtgtaga ttttaatgta gccaacaccc cttttataca
    40981 catagaggaa ttactaaatg gatatggcct agatatgcta aacaaaataa tagaaggtac
    41041 attcaatgcc aatagtatta caaatataaa agaaacaaat ctatcataca acagtttaat
    41101 aataaacttt atgacccaaa acttagaact tttggactct gttaaattat tcaactatgt
    41161 caattcttcc ttatttacaa taatagaatt aatagatgaa aacttaaaga gcaaatttaa
    41221 gctaggatat atactacatt gctcttttat gatagaaaga tgttttaaaa atacaacttt
    41281 tccatataat aacattactg aattaattag taaaaatcat gaactatata gtttaatacg
    41341 tgaagccttt aagccaacag aagcttattt taatattttg ataccagacg aagaaattgc
    41401 ttatataata gacatgatta atgagtatct aaataaataa aatttatagc acttattttg
    41461 tgcatattaa ttaatatatg aaatggagga cgagattttg aaaggaaaaa taaaacagtt
    41521 aacaatactt gcattaattt ttatatttat aactcctgta tttgcatttg cagatactcc
    41581 accagtacca aattcttcaa gagcagcact acttatagac caggaaacta aaagaatatt
    41641 atttgaaaaa aacattgatg aaaaaatgcc attagcgagt ttaagtaaaa tgatgacctt
    41701 tttattggca atagaagcag ttgataagaa tcaagtaaaa gagactgata tggtgaaaat
    41761 agacaagtct acagcttcag taggaggttc tacttgtaaa cttaaagatg gtgatgaaat
    41821 atctctagga gagcttatgc agggtctaat gctagtatct ggaaatgatg ctgctatagc
    41881 catagctaaa catattggta agactgaaaa gaattttgta aatatgatga acaagaaagc
    41941 agaagaaata ggaatgattg atacgtatta ttttaatcca aatgggcttc ctatatatac
    42001 tgacccagaa cataaagaac ctcctattga aaatatgtca actgctcacg atatagttac
    42061 acttggaaag tatatgtatg accattatga aaatcaagtt acaagaataa ccactatgca
    42121 agtatataat gatactaaaa aggattttac acattataac acaaatcccc tacttgtttc
    42181 agtacctggt gttgatggaa taaagacagg ctatacagat aatgctggat attgccttgc
    42241 attttctatg atggtaccta aagatgctaa aaacgaaaga aatcatagat tgataggtgt
    42301 tgtacttggt gatggtaaca agaaaaatag aatttcttct tccgctactt tattgaaata
    42361 tggtaaagat aatttccatt ctaaaaagat agcacataag ggtgatatta tagagactcc
    42421 ttgtgttgat ggtattgatg attttaagat aacagttaag gttgataaag atttgtatgg
    42481 tgttgtttca gataacgaaa atatcaatcc taaggtagtg tttaaaaata tgaattatcc
    42541 tatccataag ggagatattg ttggtgttgc aaagtattat aatgactctg gtaaatttgt
    42601 tggaagtgtt gatgtaaaaa gtgaaagtaa tataggatgt attcctttga aggataagat
    42661 taagattaag gttgctaaga taaataaaaa acttgagatt aaaaattctg tttgttttaa
    42721 agtatagtaa agcatactat taaaagagta tattaaattt ataaatactc cactttgtgg
    42781 agtttttttg ttttttacaa aaaattaaaa tagtggcaac aattgaggat gtgagggaga
    42841 gttgccacta ttttgtatag ggtattatat tatgttttta agctagtttt actatcttct
    42901 atgtttatat aataactctt gtgtgtgaat ttaattgata attaatatga aaattatgtg
    42961 aagttttgat attaggttat actttgaact ttatctaaca ttttaaattt tatatctcta
    43021 aattatctaa aattactata tcaattaact ttacatgata aaaaggctgt gtataaattt
    43081 atttttaaaa attttactca cagccttaaa ttaaattttc tatttaaagt atatataaat
    43141 tttattaaat aatttgcttt ttatttctta tttcataaat cctagtattc ttgataacat
    43201 taatgcaatt gtagtaggta ctttccctgc ttcaaatgta gctgttgctg tgtttaatgc
    43261 tgttactgca tcatctattt ctttttgtgt tgcatctgct ttatctaata ttgcttgtgc
    43321 ttcatctatt gctgttttat atgtcgcttt tgatcctac
//
LOCUS       DAELXX010000022        39746 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.022, whole genome shotgun sequence.
ACCESSION   DAELXX010000022 DAELXX010000000
VERSION     DAELXX010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 39746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39746
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.022"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(269..1870)
                     /locus_tag="KPH29_001125"
     CDS             complement(269..1870)
                     /locus_tag="KPH29_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG1466750.1"
                     /translation="MIYDLILKNGFIIDGTGNPGFYGDIAIKDNLIAKIDSKINSNTN
                     KEIDCCGKVITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIARQIKE
                     HDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGIDGVMDDLRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDNKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGIIKEGLIADIAVIDMDNYSYSRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
     gene            complement(1981..2658)
                     /locus_tag="KPH29_001126"
     CDS             complement(1981..2658)
                     /locus_tag="KPH29_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466751.1"
                     /translation="MSKVKSIYNEEYLPFMIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKLSD"
     gene            complement(2671..3366)
                     /locus_tag="KPH29_001127"
     CDS             complement(2671..3366)
                     /locus_tag="KPH29_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBG1466752.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            complement(3418..4617)
                     /locus_tag="KPH29_001128"
     CDS             complement(3418..4617)
                     /locus_tag="KPH29_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HBG1466753.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDICRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARNLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            complement(4878..6923)
                     /locus_tag="KPH29_001129"
     CDS             complement(4878..6923)
                     /locus_tag="KPH29_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG1466754.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNILIVIVHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQVLEDKIQNKLQSKGFVSKYTFESVVG
                     ESKIIKEKINIARKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKCFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILNSIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
     gene            complement(7334..8143)
                     /locus_tag="KPH29_001130"
     CDS             complement(7334..8143)
                     /locus_tag="KPH29_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG1466755.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTFVGFSMGGAIV
                     LRYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFEYSGHAIFYDELKLFNQEFLQFLEE"
     gene            complement(8227..8760)
                     /locus_tag="KPH29_001131"
     CDS             complement(8227..8760)
                     /locus_tag="KPH29_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG1466756.1"
                     /translation="MSDAHYDMHKRNGYSSDEPYPEIKVLGLNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            complement(8887..10962)
                     /locus_tag="KPH29_001132"
     CDS             complement(8887..10962)
                     /locus_tag="KPH29_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1466757.1"
                     /translation="MLKGLSFRNARRQAGKYLIYWCSLIGSVALIYAFNALIFSDVMQ
                     ELSSILNNGGSDEFSYMIIIFSFIITFILGWFVSYMMNFILKKRSREISTYMILGVDK
                     KQIVKMLFIENLLLGASALIVGWILGIFVSKVLESIVINMFHGKYSLSPDFSLKAAGM
                     TFLYFCIIYLIAQLKSNKKLVKMKLINLLNYDRYNENVLIHQTKTKSIMFCVSVLCGG
                     MGIFFFSIPSGRLTDVFLGLLLIVFCLIVLFMGFGSIAQNMFEKGMNWKYKKSHLFLY
                     RLLASKINGISIIMGIIATLFTVSIACIGIANSFYSVMNKSVDLQQFDVSIFHPNESY
                     DFSSYEGYLSETKGVKKDYSYTLYTEEKTSFMKVRNNILSKYLKDIGRDLQYKDYTFA
                     ENQFDTFMKYSDYNILRNMLGLNPIKMDKNQFIIHCMPYLADAYKNYIGEKYKLTISD
                     KDLSIAGIYIENFSQYGGYGNGQEYIIVIPDYLIDNMKPLYSLYVAITSEPLDSAYFT
                     EFQKKFDTLENLNSGVVVAGNQGFVSKLRYDNKDYISGKYAMQSTGQAIILILPLFYL
                     ALIICIIGTVILSIQLLSENNIIIKHYGMLRTLGMIDSEVIKILKKHVCLYFSIPLIP
                     AVIFGGILVSVFSYNMFNISFDAPVFFNIQIPILHAVAITLINFVVIYCIYVFITYVA
                     LRKEILHIK"
     gene            complement(10965..11711)
                     /locus_tag="KPH29_001133"
     CDS             complement(10965..11711)
                     /locus_tag="KPH29_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1466758.1"
                     /translation="MLLRVENIEKYYGNQKILTKALNDVSFQINTGEFVSIMGTSGSG
                     KTTLLNCIATIDTVSAGKIYLENQDITELSEENLTVFRRDNLGFVFQDSNLLDTLTLE
                     ENIALPLTIKKVSPVEITNSVYNIATQLKIEEILSKFPYEVSGGQRQRCAVARAVVTE
                     PKLILADEPTGALDSNSSKTLMELLETINTILEATILLVTHDAISASYTERILFLRDG
                     KIFNELRKGEKSRLTFYHEILDALSLMGGD"
     gene            complement(11725..12120)
                     /locus_tag="KPH29_001134"
     CDS             complement(11725..12120)
                     /locus_tag="KPH29_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HBG1466759.1"
                     /translation="MKKRILTILCVVLVLFISITGLDSMGFFSRSIIPLRSEYASFIR
                     KNGTITEVKFTGPFNHNNIDLNKNVEDTNKNGDDNELTIKYDIEVKKSLISSNEPTIQ
                     IQDSKESIYVYILNFSDKTIIIKNGKIVD"
     gene            complement(12233..13255)
                     /locus_tag="KPH29_001135"
     CDS             complement(12233..13255)
                     /locus_tag="KPH29_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG1466760.1"
                     /translation="MRFCNFLQDKVLVILLNICCAISLVLYLHLIGIGQGELTLLIAA
                     WVAILFCWICFCYQKQKKEIIKLRRIVDELDKKYLFIEMIEKPTSNIEREYYNIIRLS
                     LKSMTDHVSQIHREMYEYRQFIEQWVHEIKHPVTTSELICENYKNEHTRKIRFQMEEI
                     ERHIERALYYARLGHVEKDYLISEITLKSIVEEVLAKNKLLLIQNNIHINIQDLEYSV
                     YTDKKWIVFILNQIIINSVQYKSDSAEISFFAIKEDKSVKFTIKDNGIGIKESEIYRI
                     FELGFTGSNGRTTNNSTGIGLYLCYELSKKMGIDIDVSSSLGEYTAITLNFPINTAIN
                     MQKQCN"
     gene            complement(13252..13923)
                     /locus_tag="KPH29_001136"
     CDS             complement(13252..13923)
                     /locus_tag="KPH29_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1466761.1"
                     /translation="MILTNILIIEDDVFICKELMQFFENNGYSTQTLDTNNCDVLQVI
                     QESTPDLILLDINLPNIDGFSLCRQIRNCSTIPIIFITARDSAIDELNGLTMGGDDYI
                     TKPYNLPLLLARVKNLLKRVDSSDKQKIKYREIILNLISATVEYQNAVIELSRTELKI
                     MYYLFQNVGSIVPRIDLVEYLWDNHIHIDDNALSVNITRLREKLRGIGITDLIITKRG
                     MGYRI"
     gene            complement(14009..15358)
                     /locus_tag="KPH29_001137"
     CDS             complement(14009..15358)
                     /locus_tag="KPH29_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBG1466762.1"
                     /translation="MELKNFKDILFKKALSEGFEECEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNRANSIEEIKPCEISKLGVDNALSKV
                     GGKSIPSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIDKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEVIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            complement(15370..16752)
                     /locus_tag="KPH29_001138"
     CDS             complement(15370..16752)
                     /locus_tag="KPH29_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBG1466763.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNRDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIENGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDRPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMVGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            16996..18210
                     /locus_tag="KPH29_001139"
     CDS             16996..18210
                     /locus_tag="KPH29_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBG1466764.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIKLYGNKCDYAKRDTLLYTANKLEIKAIKEEYNLRKENGFDVEYIDE
                     STNPFSFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            18387..20300
                     /locus_tag="KPH29_001140"
     CDS             18387..20300
                     /locus_tag="KPH29_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG1466765.1"
                     /translation="MKYGGIIMKEILVIAPTKGTYEKSIHIVKKNKYTNIDVVFGNLK
                     EGIPLAEKSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFDNIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQV
                     MRIGGDKVIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSIL
                     LEKVSNEYTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRS
                     TLWRKLKEIEDDV"
     gene            20571..21545
                     /locus_tag="KPH29_001141"
     CDS             20571..21545
                     /locus_tag="KPH29_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBG1466766.1"
                     /translation="MHILKSVKKIPGGLMVCPLLLGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIVALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            21566..21841
                     /locus_tag="KPH29_001142"
     CDS             21566..21841
                     /locus_tag="KPH29_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG1466767.1"
                     /translation="MNAIIHANEKDNLVTCVRPLVKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            21854..23017
                     /locus_tag="KPH29_001143"
     CDS             21854..23017
                     /locus_tag="KPH29_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG1466768.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VDYVVIQECGGTLKTVSKGTEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKAGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            23354..25634
                     /locus_tag="KPH29_001144"
                     /pseudo
     CDS             23354..25634
                     /locus_tag="KPH29_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439637.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            complement(25729..27069)
                     /locus_tag="KPH29_001145"
     CDS             complement(25729..27069)
                     /locus_tag="KPH29_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG1466769.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEDILFILGASKDSIFYAKDYMSVILIGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVTYYFIR
                     GKSTLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     IVQVFPEVFVGIFNKDSDLMDIAVRGISINLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(27081..27863)
                     /locus_tag="KPH29_001146"
     CDS             complement(27081..27863)
                     /locus_tag="KPH29_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1466770.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            complement(27878..28900)
                     /locus_tag="KPH29_001147"
     CDS             complement(27878..28900)
                     /locus_tag="KPH29_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBG1466771.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            complement(28906..29919)
                     /locus_tag="KPH29_001148"
     CDS             complement(28906..29919)
                     /locus_tag="KPH29_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBG1466772.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFVAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTNFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            complement(30047..30985)
                     /locus_tag="KPH29_001149"
     CDS             complement(30047..30985)
                     /locus_tag="KPH29_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG1466773.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEAKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLARPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATEADKKDLENSAIWSQIRAV
                     KDGNVTILDAAPFFSQSYNPIGKELLLESVKNELTK"
     gene            31255..31719
                     /locus_tag="KPH29_001150"
     CDS             31255..31719
                     /locus_tag="KPH29_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466774.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILAGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTGKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            complement(31831..32148)
                     /locus_tag="KPH29_001151"
     CDS             complement(31831..32148)
                     /locus_tag="KPH29_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG1466775.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(32161..32892)
                     /gene="chbG"
                     /locus_tag="KPH29_001152"
     CDS             complement(32161..32892)
                     /gene="chbG"
                     /locus_tag="KPH29_001152"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBG1466776.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLVSLKPVVEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            complement(32918..34228)
                     /locus_tag="KPH29_001153"
     CDS             complement(32918..34228)
                     /locus_tag="KPH29_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBG1466777.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNNGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLVAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            complement(34305..35618)
                     /locus_tag="KPH29_001154"
     CDS             complement(34305..35618)
                     /locus_tag="KPH29_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG1466778.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            complement(35640..35951)
                     /locus_tag="KPH29_001155"
     CDS             complement(35640..35951)
                     /locus_tag="KPH29_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG1466779.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            complement(36151..36855)
                     /locus_tag="KPH29_001156"
     CDS             complement(36151..36855)
                     /locus_tag="KPH29_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG1466780.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDARVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCGYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            complement(36950..38311)
                     /locus_tag="KPH29_001157"
     CDS             complement(36950..38311)
                     /locus_tag="KPH29_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG1466781.1"
                     /translation="MKDRINLTEGRITEKLLKLSLPIMGTSFIQMGYNMIDMIWVGKA
                     GSKAVAAVGTAGFFPWLAMAFIMISKVGGEIKVAQSIGANNISTTKSYMKSAIEINII
                     LAIIYTISLIVLNKQLIGFFRLGDLEVITMSRQYLIIVALGMVFYFINPVFTAIFNGL
                     GNSKTPFRINTVGLITNIILDPILIFGWGFAPKLGVAGAAIATVFAQIVVTTCFIYII
                     VKSKEEYFKLRLFKEIETKYYKVLYKLGLPIAIQSGMFTVFSMLLGVIVASWGPVAVA
                     VQKVGSQIEAISWMTAEGLSVALGSFVGQNYGAKKYSRINKGCKIAITVSSVLGIITT
                     LVLVFAGESIFSIFIDESEAIEKGAIYLKILGYSQFFMCLEIITTGAFKGLGRTYIPS
                     IIITILTGARVPLAYFISRPEMLGLNGVWWSITLSSILKGILMISLFISLLKLGKLYK
                     MSE"
     gene            38810..>39746
                     /locus_tag="KPH29_001158"
     CDS             38810..>39746
                     /locus_tag="KPH29_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG1466782.1"
                     /translation="MKINKKFNRVDMQVSILTAIIVIISSSCVYFFNYTLTYKSMIYS
                     LSERSKHIYEYVERNIDKNTFTEINSKEDQSKKSYKSSKILLESVKNTTGVMYLYTAK
                     KTKDGSLVYVVDGLNSSRSDFRNAGDLIEKEIWGDLNKALDNHILLPKKIKSTSWGYI
                     FISYFPIHNDVGKVVGVIGIEFEAKHQYNTFKFISIVTPIIALLTCLISALIAVILFK
                     RISNPTYTDFANTDYLTGLKNRNAFEIDMNNLNNSNLKNLISIISIDLDGLKKINDKF
                     GHQTGDLYIKQASKIIQDVINKKYIVYRIGGDEYII"
BASE COUNT        13756 a         6697 c         4833 g        14460 t
ORIGIN      
        1 tagttatcat tttttatttc taaaaatctt ttctcaaatg atttagttat ctatttatcc
       61 aattttttac ataatttaat gattaaaaat attaattttt tcactataaa gtataaatat
      121 tttttgtata aatattatct gattattaaa ataaatatta atactataaa taaaaacgaa
      181 gcatgatttt tatttaaata aatcccaaac ttcgtcttta cttacttaga tatttattta
      241 ctttctaaat cctattattt ttaaaacctt atctttttaa tacttcacca catttattct
      301 cagtaatctc tccattttct aacgcaattt ttccatttac aattacatat tcaacaccat
      361 tggctagtaa ttgtggcttt gaaaaatcaa tatcttctga cctagaataa ctatagttat
      421 ccatgtcaat aactgctata tcagctatca acccttcttt aataatacct ctatttttta
      481 ttttaaattg cctagctggt aatgaagtaa ctcttcttac aatctcttcc aatctaacac
      541 cagactttct gtacatattg aagaaaattg gaaaagctcc tcttctttgt agttcaaacc
      601 atgacatatc cccacccaca ctaaacaaac aatctgaacc aaccataaca aatggattat
      661 caattatttt attatcatgt agtttatctg ggaaatcagc tctatacatt cctcctaaac
      721 aaaagataag ttcttcgtct ccatctaaaa gtaaatcgag taataacttg tcctcatcta
      781 ttcctttttg tgtagctatt tcacgtattg atttcttttc catattaata tctttagtgt
      841 ttagaagtat tatactcccc ctatctccaa gtataaatgc ttcttttaat ataagtgttc
      901 ttccctcttc atttctaaga tcatccatta ccccatctat acctaattca aatagactag
      961 atgatattgc cataataaat tgaattactc tttttttctt catacaatgc ccagaacttc
     1021 ttggtactgt gtcaaccatt atttcaacac cattatacct agcctttaat atctcatcaa
     1081 atgcttctat ttcaactgga cttaagtgag atacttgaac cctagcacca cttttttgcc
     1141 caacctctat ggcttctttt actgcattat atcttcctat gtaatctcta atatgagatg
     1201 tatatatacc atcatgctct ttaatctgcc ttgcaatatc aacaagttca tcaatatcag
     1261 catacttact tggtatatat gccaatccag tagatattcc aaaagcacca cttttcattc
     1321 catcatttat tatatctgta atttcatttt tttccttttc tgtaggaggt ctatccttag
     1381 aacctcccat aacactccat cttatagtcc catgccccaa caagaatccc atattgacag
     1441 aaataccact tttagatata aggtcacaat attctgtaaa attattccaa catttatttt
     1501 tatctataag atactttttt tcttcttcaa gtaatagacc atttttatac atatattcat
     1561 atacattttc agaagaatat ggtgttatag aatgtccaca attaccattt atagttgttg
     1621 taactccttg ttttagaaac ttttcaaatt taccatctag tattgctact atttcttcat
     1681 gaacatgagg gtcaataaat ccaggtgtta taacttttcc acaacaatca atctctttat
     1741 ttgtattgct atttatctta gaatctattt ttgctattaa gttgtcttta attgctatat
     1801 ctccataaaa accaggatta cctgtaccat ctattataaa tccatttttt aagattaaat
     1861 catatatcaa aatattccct ccttaaaaac caatttttaa ataatcttaa tgtaattttt
     1921 atatttgaaa atcaaattat tatatttcat atttgaaaat tcactattat ttcattgtaa
     1981 ttagtcacta agcttatctt tatacatagc tcttgcaatt aatatagtac caaatacaca
     2041 tataagaact actagagact gtggtattat accttgcttt gataatataa tagtcaatac
     2101 agatattaca attgctacta gacctaattt tttatcttgt aaaaatactt gtccaaaaac
     2161 tgacccaaat agagctggta aaattaagtt taatttttct actacttcag ctggaatttt
     2221 agataatacg cttcctccta aaattacccc tatagttagt atacttatat tcactaaaat
     2281 agatacagct attcctattg tagatattat cgagccttct tctgtacctg attcaacttc
     2341 tgctgccttt tgagctgcta ttgaacatgg tagcctcata ttagatatat taccagaaag
     2401 aaaactcata taagttcctg ggatacctaa gattggtgaa taagaaactg gttctattat
     2461 gtaatttgga gcactcatac tgacaattgc cattgtactt gctaataatg ctgatattgg
     2521 aggcataata ccaaatccaa agctcaaaat tattccagga actaatgctg ctataatacc
     2581 taaagatagt gttagtcttc cataccttat cataaaaggt aaatattctt cattgtatat
     2641 agattttact ttactcataa aactcccccc ttatattact gaagctatta tcattccgct
     2701 aaacattgat attgtaagcc cccactctct taaccatttt atatttttct tcttttcaag
     2761 catacacaat attaccatta tagccattcc acttatacat gcaattgttc cattatcaaa
     2821 acgcaacacc ctatcagcat ttagatatgc aaatgaaccc aacattgctc ctagagatat
     2881 tattggtata aatgatttct tgccactaga tataacattg gttatcttat ctagtttatg
     2941 tccaaatagt aatgtgaata ttatccatcc taaagaacct aatatcatag tccaaactgc
     3001 atttgtaaat gcaagttttg tcatttcagg agaacctaaa gttataccta atgcatctgt
     3061 tccaaatcca gctgacattg attcaaaaat tacagaacct ataaatgata atctcatcca
     3121 tgctataggc ccacccatag ttactataag cgataccatt ccagcaaata tagtcattga
     3181 aggcccaata gctgaaatag cactactctt aacagctgac tttatttgtt tatctgttat
     3241 tcccattgtt ttaccaactt tgcaagattt tatagcaaat ataagggatt gtaccactac
     3301 tattgatact gctactgtac tcgctatcca caaaagtgga tgattagcta attttaaata
     3361 atccatttat aaaacctccc tataaatcct caaattttat taatatttct aatatatcta
     3421 tttccaaacc tgttttagta ctctaagtat atttccacct agtaccttct ctatttcctc
     3481 atcactatat ccatgcttta ctaaatatct gacaatgttt tttgaagttt ctgttgggtt
     3541 ctcaagacct tttacatatt ctactttggg atgttctttt gtacctgtta ttttttttat
     3601 atcaaattta gcagctagta ctgagtgtaa ttttacatgg tcgccatata ctgtatctgg
     3661 tccaaaagct acatggtcta ttcctactaa atttttaata tactcaaaat gttccataaa
     3721 tgactctatt gaatgttctt tattcttctc tgttattgta gtatgaggtg ctgcttctac
     3781 acctataacc cctccttttt tagcacatgc tattaatact tcatctggag ttagtctctt
     3841 tgtgttccat aaatttcttg ccccaacatg gcttaatatt attgggtcct tgctatattc
     3901 tattacatca agtgtagttt gaactccaac gtgagagcaa tctattgcca ttccaatttt
     3961 attcattctt tcaacagctt tttttccgaa atttgtaaga cctccatctt tctcttcttt
     4021 tagtcctgaa cctaatgcat tggattcgct gtatgttata cccattaacc ttataccaaa
     4081 tccatacagt atatctattc tatcaagttc attttctatt ggtgctgcac cttctaatgt
     4141 tggtatcaat gctactttac cttcactttt agctctgcat atatcttcta ctttttcaca
     4201 ctttatgaca aaatcttgat gagctaaatc acatagtctc atgcctaaat ctattaatat
     4261 atcatcccat ttccaccctg aatttgatgt tatcgtacat acaccatcca ttaaattatc
     4321 aaatatagca tcataatatc ctcttgataa tgcttcataa gcgcatcttt gtttaccttc
     4381 cctattatat tcaaatatat ctctttctag gtgttctgga aataatactg gatgttcatg
     4441 taatgatata aatatttttt catccactat acttttaacc ttagcttctt gaatatcatc
     4501 taattcaata aggtactctt taactctttt atctccttta cacatctcaa tagatgaaat
     4561 atcttttcct ttttctaaat aactataggc ttcatatcct ttgtaagctg acttcataac
     4621 aacatcctcc aagttctaca attttttata tttttcctat gtagcaattg atatgccaaa
     4681 atataaattt taattgtata ataaatcttg caaaaaataa aaacagtagt aaggttgata
     4741 tttactactg ttttattaaa aataatcttc ttagattatt tattcaatat ttttgaatta
     4801 tcttaatctt aaagataatt ttatttattt ctatccatta tagggatttt tttagggtgt
     4861 tttgcctttt tagtccccta tatcactccc ttaattttta catatgatat aaagttcttt
     4921 ccttcatcag ttataaaact accttgtttt tttactccag ataaaagtaa accatggctc
     4981 tttaacttat ctattctttt tctaatttgg tctacactta aaactatatc tttttctttt
     5041 aaaatctctt gcagtttatt tcttcctaat aatatatttt tattattcca agtttctata
     5101 ctgttcaata tacatatact ttcctcaaag aaattagatt gtttgaaatc taagataatt
     5161 gaatcgaaat tttcattaac taaagtattg tttttttcaa atctaaattg ttctggcaaa
     5221 tgctcgtaat ctactctatc ttcctcaact atagtatcaa tataataaat taaattttta
     5281 agctctctta catttcctgg ccattcatac aatgttaaag cttgcattga ttcctctgta
     5341 aaacatttgt tgctatttat ttcatctaaa aaatacttac ttataagagg tatatcttcc
     5401 ttacgttctc taagtctagg tatctctata tgtaatacat taatcctata atacaaatct
     5461 tctctaaaag aaccttcttt aatttttttc tttaaatctt tatttgttgc agctataatt
     5521 ctaacatcta ttggtataat tttacttccg cccattctca taacttcttt ttcctgtaat
     5581 actcttagca atctctgctg tacatctaaa gatatatcac ctatctcatc taaaaaaatt
     5641 gttcctgtat gcgctctttc aaatataccc atttttccac cttttattgc tcctgtaaaa
     5701 cttccttcat catagccaaa aagttcactt tcaataagtg tatctgacaa agaagataga
     5761 ttaactgcaa caaatggctt gttttttctt agagattcat tatgtattgc ctgggcaaat
     5821 atttcttttc ctgttccatt ttctccaagt atcaacacag aaaagtctgt agttgctatt
     5881 tttcttgcaa tattaatttt ttcttttatt attttactct cacctacaac actctcaaat
     5941 gtatactttg atacaaaacc ttttgactga agtttatttt gaatcttgtc ctcaagcact
     6001 tgaattgcac ttatattttt aatgcttata atactcttta ttcgttcatt attttcataa
     6061 acatttactt tattaattat cagttttttg ttatttaaat ttactacttc atcattaact
     6121 tcatcttctt gaaaaaatat ttttttgaca accttatcac taaataagtc cataaagtta
     6181 ttagatataa tttcattttg gtctattcca actagattag aaaatacttt gttgcaatat
     6241 ataaattttc ctaatttatc aacagaggca ataccttcat ctataatctc caaaaaactc
     6301 ttcatggttt tattcatagt tgtataatac ctatatccac ttactgtatc ttcattgtat
     6361 ttttccttaa taatatgtaa tttatctata gatatattta gttttgtaaa gatttctata
     6421 actgtattta tatcaattac cttatcacct atgtcaatta tttgctttat gttttgtggt
     6481 atgctgtttc tgcttcccgt aataattcca atctcacatt tagacttatc acaacctgga
     6541 taatatggta ttagattaat atgaattaat cctagttttc taattatctt ggcagtttca
     6601 tctgctgact ctttataagc atctataacc ataacatcag aatcattttc tatacttatt
     6661 atctgattta tattttcaat atttatagta cgatgtacta ttacaataag tatattttta
     6721 tcaatattat tatgtatata ttcctttata tcattgtctg aacatactac aagctcatag
     6781 cttgacagtt caatatttat cctatttaca tataccaagt tatctatatt acaaaattct
     6841 ccaaatatat tttccaattg ttttgatata tctatattta ttttgtcatc agttgataca
     6901 aataaaatat tatgtttttt catagtccca cctcctgttt aacgagtata gaaagtaata
     6961 atttggtcta ttgtagcact ttttaaaaaa gttttaaatc ttaaaaatca aattttgtac
     7021 catttactat tattttccat aataaaataa tgtaagctat cttttttcac atataattta
     7081 atttctaaaa cggttagttt gacattattg gaatataaac aaaaatttaa tataaattcg
     7141 ttaatatatt aatacatttt taagaatgac aaggaataaa ttataacaaa ttttaggaat
     7201 atataataat agggtctcag aacttagtta caagttctaa aaccctattt gtttagtgta
     7261 aaaaacatca taaaatttag cttaaatctt ttcacataat attactcttt ttctaagtga
     7321 taataaaata tatctattcc tctaaaaact gtaaaaattc ctgattaaat agtttaagct
     7381 catcatagaa tatagcatga ccactatact caaaagtata taaaattgaa tcttctattt
     7441 tttcattcat aaaaactgca aattcatacg gacatatttc atctaatttc ccatgaaata
     7501 taccagtagg cactcttaca cattttaaat cattaaataa ttcttcatct cttaaagata
     7561 cagcagttcc aatagtgcca atacctgatg cactaaaacc aatatcatta aaccatcttc
     7621 taaagctttc tggtggattt gatgcaaata ctttttctcc aaaatcggtt accatttctg
     7681 gtctatcagt acatgcttgt gctatcaatt tatttacatc ttcacgtgtc ataccatatg
     7741 gaaattctgg aggtctttgt acaaatgatg gtgcagcagc tgcagctaat accaattttg
     7801 acactccata tccattaaat aaactcatat acctaagtac gattgctcct cccattgaaa
     7861 aaccaacaaa ggtaaagtct tttaatccaa tagcatgtac caccttataa atatcgtctg
     7921 ctaattggct ataagtatat cctcctgatg ttgcatcaga tttaccaaaa cctcttaaat
     7981 caattgaaac tgttctatat ccgagttttg gcaatatatt tgtttgatat tcaaatatct
     8041 tatgtcctaa gggccaacca tgtataaaaa gtacagtttt tttagcactc ggattcaaat
     8101 catatactgc tatgtttata tcattcacat atacataata catatcaaca gcaactccct
     8161 tcaagaaaat gtatgatggt tattttatat atatgaataa aatgatgaat aactaaattt
     8221 ttaaatttat ttacaatact tctctattgc cttttcaaat aaacttacat gaagtttttc
     8281 atctaggatt attctattta atatagcttt tatataaggg tcttcaataa gacttatatc
     8341 tttataataa ttattaatag ccacatgctc caaatgcaaa tctagtttta atctgttaca
     8401 aatagaattt ccataacata caaaaccacc attccaatat gcaccacaag aacttgtaga
     8461 acctctatat acaggatttc cacccaataa tcttatagtc tttgctaata tctccatatg
     8521 taacatttct gttatagata tatttatcca catttcactt aactctctat gcgtttcatc
     8581 taaatcaaaa ttatggtata gatattgatt tacactcgta aattcacttg atactccggc
     8641 atagtcatcc atcaataatt ctgcataata cttatttaga cctaaaactt ttatctctgg
     8701 atatggctca tcacttgaat atccatttcg cttgtgcatg tcgtaatgtg catcactcat
     8761 aatatctcct cctttaaatt taatacttga tattaattgt atgcatcacg actttttcat
     8821 ataacttaga taataaaatc tattatatta ttttctatta taatttatgt gtcaattcac
     8881 ccaaatttat ttgatatgca atatttcttt ccttagtgct acatatgtaa taaaaacata
     8941 tatacaatat attactacaa aattaataag cgtaatggct acagcatgta agataggaat
     9001 ctgaatatta aaaaatactg gagcatcaaa agaaatatta aacatgttgt atgaaaaaac
     9061 tgataccaat attccaccaa atattactgc aggaattaat ggaatcgaaa aatataaaca
     9121 aacatgtttc tttaagattt ttattacttc actatcaatc atccccaaag ttcgaagcat
     9181 tccatagtgt tttatgataa tgttattttc gctcaataat tgaatagaga gaatgactgt
     9241 accaataatg catataatga gtgccaaata aaaaagtggt aatatcaaga taatggcttg
     9301 accagtagat tgcatagcat actttccact tatataatcc ttattatcat atcgtaattt
     9361 agaaacaaag ccttgattac ctgctactac tactccagaa ttaagatttt ctaaagtatc
     9421 aaactttttc tgaaactcag taaaataagc actatccaaa ggctcacttg taatagcaac
     9481 ataaagacta tacagaggtt tcatattatc aattaaataa tctggaatga caataatata
     9541 ttcttgtcca tttccatatc ctccatactg tgaaaagttc tctatataaa tacctgcaat
     9601 acttaagtcc ttatctgata ttgtcagttt atatttttct ccaatataat tcttatatgc
     9661 atcagcaaga taaggcatac aatggatgat aaattgattt ttgtccatct ttattgggtt
     9721 taaacccaac atatttctta gtatattgta atcactatat ttcataaatg tgtcaaactg
     9781 gttttctgca aaagtataat ccttatattg taaatctcgc cctatatctt tcaagtattt
     9841 agaaaggata ttattacgaa ccttcataaa ggatgttttt tcttctgtgt aaagagtata
     9901 agaataatcc tttttcacac ctttcgtttc tgataaatat ccttcatatg aagaaaaatc
     9961 atatgattca ttcgggtgaa aaatactcac atcaaattgt tgtaaatcca ctgatttatt
    10021 catcacagaa taaaatgagt tagctattcc aatacaagca attgatactg taaaaagagt
    10081 tgcgataata cccattataa tgcttattcc atttatcttt gaagccagta gacgatacaa
    10141 aaataagtga gatttcttat atttccaatt catccctttt tcaaacatat tctgtgcaat
    10201 agatccaaat cccataaata atacgattag acaaaataca atcaacaata aaccaagaaa
    10261 tacatctgtt aatctaccac taggtattga aaagaaaaaa atacccatac ctccacacaa
    10321 aacagacaca caaaacataa tagacttcgt ttttgtttgg tgaattaata cattttcatt
    10381 atatcgatca taattaagca aattaattaa tttcatttta acaagttttt tattactttt
    10441 taattgagca attaaatata ttatgcagaa gtataaaaaa gtcatcccag cagcttttaa
    10501 tgaaaaatcc ggagataaag aatactttcc atgaaacata ttaataacaa tagattccaa
    10561 aaccttagaa acaaatatcc ctagaatcca tccaacaata agagcagaag cacctaataa
    10621 aagattctct ataaaaagca ttttaacaat ttgtttttta tctactccta atatcatata
    10681 agtacttatt tctctgcttc tttttttcaa tataaaattc atcatataac taacaaacca
    10741 gcctaaaata aaagtaataa tgaacgaaaa tatgataatc atataagaaa actcatcgct
    10801 tcctccatta ttaagtatac tagaaagttc ttgcattacg tcggaaaata tcaaagcgtt
    10861 aaaagcataa atcaaagcta cagagcctat taaggaacac caataaataa ggtactttcc
    10921 tgcttgtcgt cttgcattac gaaaagataa tcctttcaac ataatcaatc tcctcccatc
    10981 aaagaaagtg catccaaaat ttcgtgatag aaagtaagtc tggatttttc tccttttcta
    11041 agttcgttaa aaattttccc atcccttaga aataaaattc tctcagtata actagctgaa
    11101 atagcgtcgt gtgtgacaag aagaatcgta gcctctaata tggtgtttat agtttccaaa
    11161 agttccatta aagttttaga tgaattagaa tccaatgcac cagttggttc atctgccaaa
    11221 ataagtttgg gctctgttac aactgcacga gctactgcac aacgttgcct ctgaccacca
    11281 gatacttcat aaggaaactt cgaaagtatc tcttcaattt tcaattgtgt ggcaatatta
    11341 tacacactat tagtaatttc aacaggagaa acttttttta ttgtaagtgg aagtgctata
    11401 ttttcttcta atgtcagagt atctaataaa ttagaatcct gaaaaacaaa acctaaatta
    11461 tcacgacgaa aaacagttaa gttttcttcg gatagttcag taatatcttg attttcaaga
    11521 taaatttttc cagcacttac tgtatcaatt gtagcaatac aatttaacaa agtagtttta
    11581 ccagagccag aagttcccat aatactgaca aactctcctg tattaatttg aaatgacaca
    11641 tcattcaagg cctttgtaag tattttctga ttgccataat atttctcaat gttctctact
    11701 cgtaatagca tgttaaagtc ctccttagtc aacaattttt ccatttttaa ttattattgt
    11761 cttatctgaa aaattcaaga tatatacata tatgctttct ttagagtctt gaatttgaat
    11821 agtaggttca ttagatgaaa ttagagattt ttttacttca atatcgtatt ttattgttaa
    11881 ttcattatca tctccatttt tattagtatc ttctacattt ttatttaaat caatgttatt
    11941 atgattaaag ggaccagtaa actttacttc tgtaatagtt ccattttttc taataaaaga
    12001 agcatattca gaccttagtg gtatgatact tcgtgagaaa aaacccatcg aatccaatcc
    12061 tgtaattgaa ataaacaaaa cgagtacaac acataatata gttaatatcc tttttttcat
    12121 atgtttctcc ctcctttcta ttgatattgt agcgtaaata gtaatctgaa tatatagaag
    12181 ttacttaact ttatcttaca gttttgaaag ataaagaaat agcagcgcat ttttagttgc
    12241 actgcttctg catattgata gctgtattta ttgggaaatt caatgttatt gctgtgtatt
    12301 caccaaggct ggaggacaca tctatatcaa ttcccatttt cttagacagt tcataacaaa
    12361 ggtaaagacc aatccctgta gaattgtttg tagtccttcc attactgcca gtaaaaccta
    12421 attcaaaaat tcgataaatt tcactttctt tgatgccaat accattatct tttatagtaa
    12481 acttaacact cttgtcttct tttattgcaa aaaatgaaat ttcagctgaa tcacttttgt
    12541 actgaacact attgataata atttgattta aaataaaaac tatccacttt ttatcagtat
    12601 atacactata ctccaaatcc tgtatattaa tatgaatatt attttgtata agaagcaatt
    12661 tattctttgc taatacttct tctacaattg actttaatgt tatttcagaa attaaataat
    12721 ctttttcaac atggccaagg cgagcatagt acaatgcacg ctcaatatga cgttctattt
    12781 cttccatttg aaaacgaatt tttcgtgtat gttcattttt ataattttca catataagtt
    12841 ctgatgttgt aacaggatgt tttatctcat gtacccattg ttcaatgaat tgtctatatt
    12901 cgtacatttc acgatgtatt tgagaaacat gatctgtcat agatttcaat gataatctaa
    12961 taatattgta gtattcacgt tctatgttag aagtaggttt ttcaatcatt tcaataaaca
    13021 gatatttttt atccaattcg tctacaatgc gacgtaactt aataatttct tttttctgtt
    13081 tttgataaca aaaacatatc caacaaaata aaattgcgac ccaagcggct ataagaagag
    13141 tcaactctcc ttgaccaatc cctataagat gaagatatag cactaatgaa attgcacaac
    13201 aaatatttaa gagtataact aatactttat cttgaagaaa attacaaaac ctcatatcct
    13261 atatcccatc ccccgttttg taatgattaa gtcagtaata cctatacccc ttaacttttc
    13321 tctaagcctt gtaatgttta cacttaaagc attatcatca atatgaatat gattgtccca
    13381 taaatattct accaaatcta tacgaggcac aatactccct acattctgaa acaaataata
    13441 catgattttt agttcagttc ttgaaagttc tataacagca ttttggtatt ctactgtagc
    13501 tgaaataagg ttgagtataa tctctctata tttaattttc tgtttatctg atgaatctac
    13561 tcttttaagt aggttcttta cccttgctaa aagcaaaggt aagttatatg gttttgtaat
    13621 atagtcatct ccccccatag tcaaaccatt tagttcatca attgcactat ctcttgctgt
    13681 aataaaaata ataggtatag tagaacaatt cctaatctgt ctacataaag aaaaaccatc
    13741 tatattaggc aaattgatat ccaagagaat taagtcaggt gttgattctt ggattacttg
    13801 aagtacatca caattatttg tatccaatgt ttgtgtacta tagccattat tttcaaagaa
    13861 ttgcattaat tccttacaga tgaacacatc atcttcaatt atcaaaatat tggttaaaat
    13921 aataaatacc tcatttcttt agtttatatt gctttaattt tgtaagtaat atatttttcc
    13981 atatattact tacaaaatta ctttttaatt attcatcttt tccagctact gatagctctt
    14041 taataacaac tgaaggtgaa cctatactac tcattggaaa ttctaaatct tcccctataa
    14101 cttcaatatc tttcaacagg tcaaaatagt ttcctgctac tgtaatttgc tccactggaa
    14161 atactttttt tccttctttg atataaaagc ctttggcagc taaagaaaag tctcctgtta
    14221 ctgaatttgc acctgaatga agccctgcaa agctagtaac catcaatcct tcatctatat
    14281 cctttataat ttcatctaaa gatttatctc ctttatctat ataaaaatta gttggatata
    14341 tgcttatagg tgatgaatat gaagatttta ttccatttcc tgtagtcttt acacctgcct
    14401 tattagccgt ttttaagtta tgaagtaaag ttataagctt tccatttaaa acaacttctt
    14461 ttttaaatgt agctactcct tcatcatcaa aaggagtaga tgccatacca ttttctaaaa
    14521 gtgggtcatc aactattgta actataggag atgctatcat gtctccttct ctatttttca
    14581 ataaagataa tcctttttga gctgaatctg cattaaatat cccagaaaat gtgctaagca
    14641 aagataccat agcttcatta agcaatatag ttttatattt acctgatgga atactcttac
    14701 cacctacttt agataaagca ttatcaacac caagttttga tatctcacaa ggttttattt
    14761 cttcaataga atttgctcta ttatatccta ttccatcata tttttgtcca ttgtcttcta
    14821 tgataggaac tacaaaccct ataagcatat tagttttatt gcttaaatta agacctttag
    14881 tattagatat accattactt gatacagaat aagatatagt acatttagat aaatttacca
    14941 ccttatctga ataagcttta gtctctcttt ccatttctaa agctaaatca atcaacttat
    15001 ctgcctctaa attttctagc tctttagaat aagtctttac atcattatag tgcttatctc
    15061 cttcatatat aaattgtaca tcttcatttt caatagtgtt tactccatct tttacatttt
    15121 ttatcaacat atcaacagct ttatcatcta aaatttctgt ataagaataa cctatctttc
    15181 catttacctt acctctaaaa gataaaccaa aagatttatc aagattgtat ttctcaactt
    15241 ctccttcata tatatttata cttaaatttt ctcctgtata ataatatatt tcacactctt
    15301 caaaaccttc acttagagcc tttttaaata atatatcttt aaaattttta agttccacaa
    15361 ttatccctcc tatcttccac ctacagtaat ttcttttacc cttatcatag gttggcctac
    15421 attagttggt atacttcctg atgatgagcc acacatcccc tgagcttgtt ttacgttatt
    15481 cccaaccata tctatattca taagtatatc gctacctttt ccaattaaac tagcacctct
    15541 cactggctct tgtatttctc cattttttat taagtaacct tctgaaactg caaaattaaa
    15601 ctctcctgtt acaggattta cagaaccacc acccattttt ttagcataaa gtccatctgg
    15661 tattgacttt ataatgtctt ctggtctatc ttctccagca gcaatataag tgttagtcat
    15721 ccttgaagtt ggtgcaaatt tgtagttctg tctacgagaa cttcctgtag gattcattcc
    15781 cattcgtcta ccattaagtt tatctatcat ataagacttc aaaattccat tttcaatcag
    15841 tatattcttt tgagttggat ttccttcatc atctatattc atagaacccc agtagtttgg
    15901 tattgtacca tcatctatag ctgtaatctt tgtggaagca atctgttgac ctaatttatc
    15961 tgcaaataca gagttcccct ttgcaactga tgtagcttcc aaagaatgtc cacaagcttc
    16021 atgaaatatt actccaccaa atccattatc tattgctacc atcatattac ctgcaggaca
    16081 atttttagca tgcaacatag tatgcgctac tctagctgat tcttttgcat aatattctgg
    16141 gtctatttct tcaaatattt caaacccttt acaacctcca ggaccttcaa atccagtttg
    16201 attttcatta cccttagatg caattgaact tatccctagt ctggttctta ttcttctatc
    16261 ctcaacatat ataccttcag tgtttgcaat taatattttt tggtctttat ccaaatagct
    16321 aacacttact tgagatatat cattactaaa ttcttttgca ctcttatatg caaccttcat
    16381 aacacttact ttatctctat ttcctattga atttggataa atttttatat tatgtatgtt
    16441 atttatttta atactgtcat ttaatattac actttttcca tcttccaatt tacctaaagc
    16501 aacagcagct ttgtatgcta catccaacag actatttaat gaattgtcac ttgtataagc
    16561 atatatactc ttcaatcctt taaatattct tattcctata ccataatttc ttccacctat
    16621 tgcattttct actttattat caattaaact aatagaatta tttatagtgt cttcttcaaa
    16681 aatctcagca aaatctcctc cagtaattag acacttttct aataccttag aagctatatt
    16741 ttttgaaagc ataaatttac ctcctacttt aaatttttaa ttaactttta ttcaatcttt
    16801 ttacttgtta acaaacatta tatactatat tacttaaaat aaggtaatta tgaatatatt
    16861 atacatatgt tttatactaa aatgttatca cattttatct atacataccc atataactag
    16921 ataattaaaa tgtataatat tatttgctat agaaaataat acactcatac ataataaatt
    16981 ttaggagtga agtatatgaa tcaagaatat gtaaaaggaa atcctatttt tactaatatt
    17041 aataaagttc caagacaatt tccatacctt actgatgata ttgatactga tgttataatt
    17101 gttggtggtg gcgtaactgg atgtatttgt gcttactatc tagctaaaaa caatataaag
    17161 tctgtcattc ttgaaaaagg aaggattgca catggaagca ctagcgtgac aacttctcta
    17221 ttacagtatg aattagatga caatttgata gatttaacag aggttatgac tctaaaagat
    17281 gctctaaaag catataacct ttgtacttca gcacttgaag aattagatac atttataaag
    17341 ctatatggaa ataagtgtga ttatgccaaa agagatactc tactttatac tgccaacaaa
    17401 cttgaaatta aggctatcaa agaagaatat aacttaagaa aagaaaatgg atttgatgtt
    17461 gaatatattg atgaatccac aaaccctttt agctttgatt taaaatcagg attgatagca
    17521 aagaatggag gacgagaatt agacccatat aaatatagtc accatttgat tgatgtaagt
    17581 ttaaaaaatg gtcttcaagt atatgaaaat acagaagtaa aaaaagtaga tttatcaaat
    17641 gataaggtta ctgctgaagt atcgtatgga tacaaagttc atggtaaaaa attaattgta
    17701 gctacagggt acaatacaag tttatttaca aaaagaaatt ttgcaactaa gtcaaatact
    17761 tttaatatag caactaaacc acttaaaaac atagcttctt ggaaaaacaa cattttaatt
    17821 agagataact gtgacccata taattatcta agaactacta aagacaatcg attaattata
    17881 ggtggtgaag atgtaagttt tgatgatata gaaaatgaaa ctttagccaa tgaaaagtat
    17941 gatattttag aacaaagatt gaaaagtatg tttaaggata taaaagacat tgaagttgaa
    18001 tataaatatt gtggttgttt tgcttcaact ttagataatt taggttttat tggtccagat
    18061 aataaaaata acaatctatg gtattgctta ggttatggag caaatggtat actctttgca
    18121 atattaggag gtataatgtt aagtgaatta tatcttggca aacaaaataa aaatatgaaa
    18181 ttatttaaag tagatagatt tgataaatag atattgtata ttaaaagact ataaaaagat
    18241 gaaactttat aattctaaaa gttaactttg atacagtctt ttataatgtt ccttttaaaa
    18301 atactttatt tataattaaa tataaagtaa aatgttaaaa tataactgat aaaatttagt
    18361 cataagataa tatttaagtt tatttgatga aatatggagg aattattatg aaagaaatac
    18421 tcgttattgc tcccactaaa ggaacctatg aaaaatcaat tcacatagtt aaaaaaaaca
    18481 aatatacaaa tattgatgtt gtatttggca atctaaaaga aggtatacct cttgctgaaa
    18541 aaagtattaa tcatggtact agaattatca taagcagagg tggaacttac aatatgttaa
    18601 aagctactta caatatacct atagttgaaa ttaaagtaga tgcttatgat attataaaaa
    18661 gttacaaaga ggtaaaaaat tccaacgaac catttggaat aattggattt aataatgtca
    18721 tctacggttt cgatattata gaagaaatat tgaataagaa aataactatg attgaaattg
    18781 aaaaagaaga agaaatttat gatgctatag aaaagtacag aaagaaaggt ataaacacat
    18841 atataggaga taccactgtt gcacatattg ttaagagact taattgtaag ggaatattaa
    18901 tcgaatctag agaagaaaac atacttagag ccattcaaca agcagaacaa atactcgaag
    18961 caaccaagga tgaacaaaaa agaagattac agatagaagc tatgacagat tttgtacatg
    19021 atggaataat aacagttgat aaaaatttta aaataacatt atttaataag agtgctgaaa
    19081 aaatatttgg tataaaaaaa gaaaatgcac tttatcataa tgttgtagat gttatcccaa
    19141 acacagttct tccacaagta gttgagacta tggaacctca aattggtgaa atacaaaata
    19201 tagggagtac aaaaattatt acaaatagag ttccaataat tgttgatggc cagattcaag
    19261 gggctgtttc aacatttcaa gatagcaagg agataagttc ttttgaacat acaataagaa
    19321 ggagcttatt aagcaaaggt ctatctgcta aatacacttt tgataatata attcatgcta
    19381 gtgaaaagac aaaagattgt atagaacttg caaaaaaata tgcttgttat gatactccta
    19441 tgattattac tggagaatct ggagttggta aagaattatt ttgtcaaagt atacataact
    19501 atagtaagcg taaaaatgca ccttttgttg ctgtaaattg tgctgccata cctccatctc
    19561 taatagagag tgagtttttt ggttatgagg aaggttcatt tacaggtgct agaaaaaaag
    19621 gtaaagcagg tgtattcgaa ctagcacatg aaggtacaat cttcctagac gaaataagtg
    19681 aaatccctct tgaacttcaa ggtcgattat tgagggtatt gcaggaaaaa caagttatga
    19741 gaattggtgg tgataaagtt attcctatag atgtaaaaat tatctgtgct tcaaataaag
    19801 acttaaaaaa tatgatgcgc aatggtttat ttagacaaga cctttatttt agaataaata
    19861 tactaacatt atatcttcct tcattaaaag aaagaaaaga agatattata aaattatcag
    19921 aattttttat acataaatat tcaagcaaat acaataaaac gcctttaatt atcactccaa
    19981 atattaaaaa ggcacttctt gaacgagaat atgaaggtaa tataagggaa ttggaaggta
    20041 tgatggaaag ggctgttatt gtggaatcat ttgactcgat tttattagaa aaggttagca
    20101 atgaatatac aaataatata gttgataaag ataatagtga ttttgaatgc agtaatttag
    20161 atttaaaaac tttagaaaaa aaatatatct ataaaattta taacgaaaat aatagaaata
    20221 ctacaaaaac ctgtgaaatt ctaaagataa ctcgctccac tctatggcgt aagttaaaag
    20281 aaatagaaga tgatgtttaa aaataataca tacctatata tatcaaaacg ttttatgttt
    20341 aattttaata cataaggcgt ttatttgttt tcaataaaaa atacaattat atgataaaaa
    20401 gaatctttgt aagtgaaatt ctcatcaatc atacctttaa ttataagtaa tctacagcat
    20461 ttcaatcgat tattaaaaat aatcatgata actaaaaata caaactctat atattggcat
    20521 agtaattgct tttataatac ctatataaaa atgaaaggga gtgatactag atgcacatct
    20581 taaaatcagt gaaaaagata ccaggaggac ttatggtctg tcccttgtta ttgggagcac
    20641 tatttaatac agtatgtcca caagctcttg aaattggagg attcacaaca tccttattta
    20701 aaactggtgc aatgagtata cttgctctat tttgtctatg caatggagct caaatcaata
    20761 tcaggcaagc tggtaagcca ttgactaaag gtatagtact tacagcaaca aaatttataa
    20821 ttggtgcagt attaggtatt attgttagta aatttattgg acctaaaggt ataattggta
    20881 ttacacctct tgccatagtt gctaccatga ctaattcaaa tggaggtcta tttgctgctc
    20941 ttgcaggtga atatggtgac tccacagatg ttggagcaat atctatctta tctctaaatg
    21001 atggtccttt ttttacaatg cttgcttttg gagtaactgg acttgcaaat gtacctatag
    21061 tagcactatt tgcagcactt ataccaatac tacttggatt tatacttggt aaccttgatg
    21121 aagatatacg agaatttctt gcacctggta caacattatt aattcctttc ttcgcttttc
    21181 ctttaggagc agcattaaac tttaatcaaa ttataactgc tggactacct ggagttgtac
    21241 ttggtttagg agttactata ataacaggta tgggtggcta ctttgtaatg aaacttatac
    21301 gtgcagaaca tccagcagta ggtgcagcta caggtacaac agccggaaat gcagctggta
    21361 ctcctgaagc tttagcagct atagacccta ctctagctac aatagcagct gtttcaacag
    21421 tacaggttgc tgctgctatt attgttacag caatatgttg tccaatgtta gtttcctatc
    21481 tagataaaag agaaaaaaag aaaaaatcta tttttaaaac aaataaaaat attgaagtaa
    21541 attaatactt aaggaggtaa aattaatgaa tgcaattatt catgcaaatg aaaaagataa
    21601 tctagttaca tgtgtcagac ctttagttaa aggtgaaaaa gtttcaattg atggaaagtc
    21661 ttacatagta aatgataata tacctgtctt tcataagatg gctacagaag atgtaaaaaa
    21721 aggtgatgtt gtatacaaat acggtgaaat tataggaatt gcaacagttg acataaaaac
    21781 tggtgactat gttcatgtac ataacattga aagtacacgt ggtcgtggag ataaaaaata
    21841 ggaggaagtt aaaatgaaat taatgggtta tttaagagaa aatggtcagt ttggtattcg
    21901 taatcatgtt ttaattattc caacatctgt atgttcaagt gagacagcta caagaatagc
    21961 atcacttgtg cctggagcaa tagcaatccc tcatcaacat ggatgttgtc aaattggttc
    22021 tgatatagag cttacagcaa agacattaat aggatttgga aaaaatccta atgttgccgc
    22081 agttttagta gttggtcttg gatgtgatgg tattcaagca aaagaattag catctgaaat
    22141 agctacaaca ggaaaaaagg tagattacgt tgttatacaa gaatgtggtg gaacattaaa
    22201 aacagtttct aagggaactg aaatagtaag taaaatggct cgtgaagtat caaaagaagt
    22261 aagagttgaa tttggtatga gtgaaattac attagcactt gaatgtggtg gttctgaccc
    22321 aacaagtgga atagcttcaa acccttcaat aggcgttgca tcaaaccttc ttgtagatga
    22381 aggtggaagt agcatactaa gtgagactac agaagtaatt ggtgcagaac atttgttggc
    22441 tacccgtttt gaagatgaag aaatgaaaga taagtttctt aaatttgtaa gtgatgtaga
    22501 aaaaagagca attgctatgg gagaagattt acgtagtggt caacctactc caggtaataa
    22561 agctggtgga ctttcaacaa ttgaagaaaa atcacttggg tgtatgtata aggcaggcaa
    22621 taagccattc aaaggtgctc ttgaatatgc tgatattgta cctcctgata aaaaaggatt
    22681 atattttatg gatactccag gtcaagatat agattctatc actggtatgg tagcaggtgg
    22741 tgcacaaatt gtaatctttt caactggtag aggtactcca acaggaagcc ctatttcacc
    22801 agttattaaa atcactggga atagtgatac atacaacaaa atgccagaca atatcgatat
    22861 aaatgcaggt agaattataa cagatggtgc taaaatagca gatataggtc aagaaatatt
    22921 taatgaaata atagaagtct gtaatggtaa acataccaaa gctgaaagtc ttggtcatag
    22981 agaatttggc atttacagaa tttcatctac attttagatt gtaacaaaat atcttttata
    23041 aatataatac ttatgtagat acataagcta aaagttcttt ctaatcctat aaaaattttc
    23101 tatactgaaa aggaactgta tcaaaattat tttgaaaaca gttccttttt taacctcttt
    23161 tactcaaaat attataaaat aagatttaaa caataaacgt gtcttgatta atctctaaga
    23221 agacataaat aaagctaaat gtaaagtgag ataaataaaa tttaatatct ttgtatgaaa
    23281 agatatttgg tataatatgt ctgagagaat tcttatttgt aaacaaataa aggatggagg
    23341 atgaaacaaa agaatgaact ttgaaaacac aaaaaataaa aaaataatag ctactttact
    23401 attgacactc cttttaatcg tgtcattaac atgtgtcaat tttattatga atctaaataa
    23461 agtaacacat aatgaaacag aacgatatct tgtagaagtt tcacaaagta tttccactac
    23521 aattaatgtg aaatgtaaaa gtatatttag gacactatat tctatgggag atacttattt
    23581 tgagattaat gatagtgaaa aagaagtcaa tagatactta tcaaaaaaat taaaattatt
    23641 tgaatttgat tggttaggta ttatagattc taatggaaat tttgtaagtg cccttaaaga
    23701 tacaggtaat gttaaaggac aagactttta tagaaaagcc cttcaaggta aagaaacaat
    23761 tactcctgtt aaatcacaat ctggtacata tggaatttta tattctgtac cactatacaa
    23821 tggcgataaa attgttggag ctgttgctgc ttggaatact attgatacta tgagaaattt
    23881 attaagtgta gaaagctttc atggtgaagg attttcccaa attatcgacc aagatggaaa
    23941 ttttattgta agttcagcaa acaaaaatgc tccaaaaaat gtggtaaatt tttttgacat
    24001 gattaaacac caaggaaaac aggttgataa aaataaactt gaagaaatga aaataaactt
    24061 aaaaaagaac aaaactggtt ctatgtatta tacattaaat gatggtattt ctaaggcaat
    24121 gcattatctt ccattacaag aagaaaactt atacctcctt tcagttgttc caacaaaaac
    24181 agcaagtgca cagtttgatt tacttctaaa aaaagcaatc ttcatcaata ttttaattgt
    24241 tatactattt ttattgctta ttatattgat tatgtttata aatcaaaaga acagggcaca
    24301 acttataaaa atcgcctatg tagactctgt aactaaaggt tacagtaaag cacgttttga
    24361 tattgaagct aaaaaaatta tacagtcatc ttcatcagaa agctattcat tggtatctat
    24421 aaatattcag aaattcaaac ttattaatga ttcttttggt agtgaagcag gagataaaac
    24481 attaaagtac atacatgatg tcattagtaa ttatctagaa ccaggagaat tgctttgtcg
    24541 agtttctaat gaccagttta atgtacttat taaaactatt cctcaaaaag aaattcttaa
    24601 gtacatagaa aatatcacca atgatattaa tagtttcaat aaaaatgaag aatgtaaata
    24661 ttttatagct ctatctatag gtgtatataa aattgatgac ccaaatctac ctttagtttc
    24721 aattcaagac cgtgcaaatg ttgcacgaaa aaataataaa ggaaacattg gaaatcatcc
    24781 actttataca tgtgtttttt atagtgattt agagcgttta aaaatgatta aagaaaaaga
    24841 aatggaaaat agaatggagg aagcattaaa taataacgaa tttatagtat atcttcagcc
    24901 taaaataagc ctcaaagata atactattgt aggtgcagaa gcattagtaa gatggcaaga
    24961 cccaaataaa ggccttatac caccagacga gtttattcct ttctttgaaa aaaatggttt
    25021 tattacaaaa ctcgaccact atgtttttga aaaagtatgt agtatgataa gaaaatggat
    25081 agataatgga gaaactccaa tgcctgtttc agttaatttt tcacgtgcta atttaaataa
    25141 tatagatttt ttagaaaaat acagaatgct tcgtaataaa tacaacattc ctgctaagct
    25201 cttagaaatt gaattaactg aaacacttgt atttgaaaat ctccaaaaat taattaatat
    25261 aatagaccaa atccatgaag aaggttttca atgttcacta gatgactttg gaagtggata
    25321 ctcttcactc aatatgttaa aagaaataaa agttgacaca tataaaactt gaccgtgctt
    25381 tttttagctc acctaatgca gataatattg ctgaaaatca tgtaatagaa tctatagtta
    25441 atttggcaaa aaaattaaat atgaatagta tatcagaagg tgttgaaaca atcttgcaaa
    25501 tggattatct taaaaaaatt aattgtgata tgatacaagg atatgttttt tcaaaacctc
    25561 tgcctcaaga aatatttgag aaaatgactt ttggaaaaaa actagaagaa accagtatgg
    25621 atagttccgt ttaaataaaa taaaaaactt gtctctataa aataatttta atcattttat
    25681 aaagacaagt ctttttattt tccaatttaa ttttattttc taaatttctt atactcggct
    25741 tgttttaaat tcccttttta agaataataa tacgattatc atagcaataa aatctgcaac
    25801 tggctgtgcc aaccaaacac catctaaatt aaattgaatt ggaagtacaa tgattaatgg
    25861 tataaataaa ataaattgtc ttaaaagagt taaaaacatt gaatgtttaa ctttacttat
    25921 acactgaaaa tacacaggtc ctacaataga aatccccatt ataggcaatg taattagatt
    25981 tatgcttata cctcttactg ctatatccat caaatctgaa tctttattaa atatacctac
    26041 aaatacttct gggaaaactt gtactattaa aaatccaaat gacaatatga caactgacac
    26101 tatgatagat aatattaaag ttttctttgc acgttcatat tgttttgcac cataattgta
    26161 tgatattata gtttgcatac cttgattcaa tccaaacaca ggcatcataa acatcaataa
    26221 aatagatgta agtgctgtca tagccccaat agctaaatct ccaccataaa cctttagaga
    26281 attgtttgta aacatgtgag ttatacttat agctacttcc atagcaaaag gtgtcattcc
    26341 tattgcaaat accttcttta ctatatcttt attcaatttt aagttacatt tttttagttt
    26401 taaagtggac ttccctctta taaaataata tgttacccaa aagaatacta ctatttgaca
    26461 tagtactgtt gctatagctg ctccttttat acctaaatta aacacaaata taaatatagg
    26521 gtccaatatc aggtttaata cacatcctac aatcattgtc cttgctgcta attttggatt
    26581 accttctgct ctaattgcat tattaagaga aaatgcaact aaattaaata tacttccaat
    26641 caatatgaca ctcatatagt cttttgcata aaatattgaa tctttacttg cacccaaaat
    26701 aaaaagtata tcttctaaga aaactaatcc taatatagtt ataattaaag agataattat
    26761 agataatgtc aaagcattac ctaatatctt ctctgcttct tctctatttc tttcccctaa
    26821 ttttatagac aatcttgtgc tagcacctac tgataccaac attccaaaag ctattattag
    26881 agtaaatact ggcatagtta caccaagacc tgcaatagct atagagccaa taccttccat
    26941 agaaccaata aaagctctat ctactacatt ataaagtgaa gttaccatca ttgctattat
    27001 tgccggtatc gaatacctaa ataataaaat ccatattttt tcatgtctta atgcttcttg
    27061 attctccatg ttgtcaccat ttacaccatg tcataagtta tacaaattgg tttatttgtt
    27121 cttgggtctg ttgctatttc tgcatcaata ttaaataatt cttttagatt atccttcgtc
    27181 ataacatcat atgcatcacc ttcacaaact attttacctt tcttaatacc tatcatgtgg
    27241 tcagcaaatc ttgctgcatt attaagttca tgtattacca tgacaatagt tcttccttgt
    27301 tttttattta gttcatctaa taattttagt atctctaatt gatgtgccaa atctaaatat
    27361 gttgtaggct catccagtaa taatatatca gtttcttgtg ccaaagccat agctatccat
    27421 gccctttgtc tttgtcctcc tgataaatct tccatatttc tatccttaaa ttctgcaatt
    27481 ccagtaactt ctaaactcca atttacaatc tctctgtctt ctttactaga attaccaaat
    27541 ccacttttat gaggaaatct tccataagaa attaattctg aaacggtaag accactagga
    27601 gcttgagggc tttgtggaag tacagccata tcctttgcta tttctttaga cttctttttg
    27661 tagatgtcct ctccatttat atatattttc ccactattag ggctaataat tctacctata
    27721 gttttaagta ttgtcgattt tccacaacca ttagccccta tgatggtagt tatttttcct
    27781 tttggtatat ttaaatttaa attttttata atatctgtgt ttccatatga tacatttaga
    27841 ttcttagtat ttatacagtt cataacaaca ccctttccta atcatttacc aacattaaat
    27901 aaataaagta cggcactcct attatagaaa ctactatacc tactggtatt tctataggtg
    27961 ctagaagatt tcttgatata gtatctgcta ttaaaagtaa taatgttcct atcaatgctg
    28021 atactggtat taatctttta tgctttggtc ctattatttt tctacctata tgtggagcta
    28081 ttaaacctaa aaatgaaata ctacctgcta ctgctgtcga tacaccagat aaaattactg
    28141 catatattat aagtcttctt ctttgttttt ctacatctac accaagactt atagatactt
    28201 catcacccaa atttaaaaca tctatatatt tagatttata aatagttata cccataaaaa
    28261 tcactataaa tggaagcaca gctattacat atttccaact ggttccccaa atgcttcctg
    28321 atgtccatgc cataacccta ttaaattctt gtgttgtaaa tttcaattgg aatatcgtca
    28381 aaattgaagt aaaagctatg tttacaccta taccaataag tagtaatctt gacgaattta
    28441 taccaccttt ccatgcaaga gaatatataa gaaatgctcc aaataaagct cctataagtg
    28501 caacaatagg cattgtaaat acagtcaagt tactcatacc ttcatataca ttaccattca
    28561 taaggtatat ataaactact acaaacaatg cagcacctga atttattccc aaaattccag
    28621 aatcagctaa atcattcttt gtaactcctt gaagtattac acctgctgtt gataatgcag
    28681 aaccaaccaa aagtgctatt actattcttg gtaatcttaa tttaaatatt gcaatttcat
    28741 gattacgctg accctgccct aaaaaagtct ttataacatc cattggttct atagaaaatg
    28801 agcccatatt taaactcaat aaaaaagtga taaaaataag caccattaaa attgatataa
    28861 caagtaatgt tttagaattt atttttataa tatttttcat agtacctatt tctccccctt
    28921 tctaacaagg tatataaata ttggcactcc tataagtgct gttatagaac ctactggtgt
    28981 ctcatatgga ggatttatca tcctacttaa tatatctgta tatacaagca atacagctcc
    29041 taatactaaa gagctcggta ttatatactt ataatctgaa cctactattc ctttagctat
    29101 ttgaggaact ataagaccta taaaaataat attaccagac actgacacag atgctccagt
    29161 cattaatatt acaagtacta tacatataaa tctaacaaag ttagtttttt gtcctaatcc
    29221 tattgccact tcttctccaa gacttaatat agttattctt ggagccataa taatagctaa
    29281 aataaatcca ataccaccag ctacaaatag caattttata ccttcccact tcactcctac
    29341 aagccctcct gatatccaga aacttaattg ttgagatagg ttaaaataca tagatattgc
    29401 tgatgccaaa gaaattagca atgtaccaag agcgactcct gcaagagcta attttacatt
    29461 attaactctt cctcttgatt tagaacttat aaaatatata aataatccac ttatacttgc
    29521 tccaataaat gcaaacatca ttacactaaa acttccatgt ataagttgag gaagtttttt
    29581 ttgaagtacc agtgatatcg aaattgcgaa tgtagctcct tgtgtaattc ctataacaga
    29641 tggttcagct ataggatttc tcgttattcc ttgcattatt gccccagaaa ctgctaaaaa
    29701 ccctccaaca agtgcagctg ctattgctct tggaagtcta acatccttta ctattctcat
    29761 atttattcca tcttccattt ttatcaaact atctatgaca gttgatagat tcatatcttt
    29821 tgccccaata gttatggata atacaattcc aaatacaagt acaactatac ttactaatat
    29881 caacaaataa gttatatttt ttttactcag cttagtcaat tatcctacct ccttttccct
    29941 tttaatcgtc agtataatat catctattaa gtattttaat tttaagattt attaaaaata
    30001 ggtgtgaaaa atgaatatca ttatctcaca cctactaagt taagaattac tttgttaatt
    30061 catttttaac tgattctaat aataactctt ttccaattgg attgtatgat tgactgaaaa
    30121 atggagcagc atcaagtatt gttacattac catcttttac tgctcttatt tgagaccaga
    30181 tagcactatt ttctaaatct tttttatctg cttctgtagc aattacaact atatggtctg
    30241 catctatatc tgttaaacct tccatactta ccattggtag tgttattcca tcctgttttg
    30301 gcatattctt aggtctagct aatttcatat catcattgat tagagtacct attccaccat
    30361 cactaaatac cataaattga cctgaacttg ccaacactgg taaataagtt ttttctccat
    30421 tcttttctat aacttcctta cctaactttg tagctttttc atcatacttc tgtagccaac
    30481 ttttagcttc ttcttctttg tcaaataact ttgaaacatc agttaacttg cttctccagt
    30541 catttgcata atctttcatc ataactacag gagctatttc ttttaattgg tcatatattt
    30601 tttcttgtct ttgactcatt attattaaat caggatttac ttctaatata gcttccatat
    30661 ccatagtatc catcattgag tgcccaacta tctttacacc tttaagctct tctcttatgt
    30721 aacttggtaa tttatcagtt tcatatgagt ctacatttgc tgttgcaaca ggcttataac
    30781 ctgctaataa tagttcttca ctacttcctg atatatcaac tattttttta ggatttgatg
    30841 gtatcttaac ctctccttta acagattgta caactcttgt ctctgattta tctttatcct
    30901 ctgtattctt atctgaacat gctgtaacaa gtactaacgt agttataata gatataaata
    30961 ttgcaagtga ttttattttt ttcatgatta cttctcctac ttgtttatta tacttacatg
    31021 atattgatta tcataagcac atatttatta tagcattttt ctatttttag tcaatattat
    31081 tttgtaaaag gataaaaatt cctaattaat cttaattata tatagcttag ataaggtttt
    31141 cttatattaa acagtatttt ttttaagaat tggacacaaa cacaatgact tttaggttaa
    31201 acaatgataa aatgcaattt atagataatg aatttagtaa ggaggattca aaacatggta
    31261 catttagtat attgtgataa tgcaggaaaa aaaggagaaa aggtcttaga taaaatcttg
    31321 gctggaacaa aaacaatggt tattagaggt gctgctggta gaaaaatacc acatagtaga
    31381 gtatttgaag atgagttact ttattttatg gaaaaaggaa ctggaaaaat atctgcaaaa
    31441 gccactgtta aaaaagtaga aaactatgtt aaattaacag aagatgaaat ttctaaaatt
    31501 ttaaatgaaa atcaaccaaa attaaattta tctgaaaagc aaaaagttcg ttggcataaa
    31561 aaatgtttat gcttagttga atttgaaaat gttgaagaaa taacacctct tgattttgac
    31621 catcaaggaa atatggatga ttggcttatt gttgaaaaaa ttgaagatgt agttgttggt
    31681 actagtatac cttacaatta tgagaagtct aaattttaaa gtatcaataa cataatacta
    31741 tattgctaaa ctctacttaa ataaaaaaag agtgcattta ataattataa aaattatttt
    31801 tatacactct tttttaataa taaatttaaa ttacttactt tctaatcttt catatacatc
    31861 tataaattct tctgctaatt gttgaaaatt catagctgtc attaaatggt cttgtgcatg
    31921 aataagtata acacttattt cagttttatt accattagct tcattttgta tcaattctgt
    31981 ttggaattta tgagctttat gaattgattc tttcgacttt tcaatacatt ctctagcttt
    32041 atctatattc ccgctctttg cttcttttat tgcttcaaat gctagccctt tcgcatcacc
    32101 tgcaaatcca attattccaa aagatatctc tatcatcttc tcatccattt attcgacctc
    32161 ctaaatatct ctatagcttg atatgactaa tccattcttt tctaaaaatt ctttgacttt
    32221 tttagatgta agtatcttgt gctctttcgt tctatcaata gcataagatg tagaatttaa
    32281 aatataatca tctaaaaaag ctgggtgaga cataatatcc actacatcat acttcattat
    32341 ttcgtctata ttttttataa aatattcttc acttacattt tccttataaa aactatctat
    32401 taaaggaact actttagaat actctaaatt atattcaaat ccacctctta ttggaagatt
    32461 atacttggtt acaatcttct ctactactgg ctttaaactt actaaagtat ggatatgatg
    32521 gtgactatct aaatgagata tatctattcc aagccctttt accctatcta tttgagcaca
    32581 aagttctcta taaatctcat cacaatccat tttttcaata atttcgtctg taatcctcct
    32641 aataaaaaaa ccgtctttat caacaatagt ttttaagtta gataataatg gtctaccaca
    32701 acttaaagtc atatgaactc cacaatttaa agttttattt tctttaagta agcctactcc
    32761 atgttctacc ccaggcatat ttgccatcat agaagtagac ctaactatac cattattatg
    32821 agctgaaatt attccatagt tcacagcttc acaatatcca aaatcatctg catttattat
    32881 aattttagtc ataccataac ctactttcta tagacaacta tctattaaat ctaggaagat
    32941 aatctttatg agcttccaac atttcgtcta atatagtttt tgctaagtca tcagatggaa
    33001 tcaatgggtt tatacaaagt gccaaaagag cagattcata atcgccatat acagctgctt
    33061 tagctaccaa tacttcaaat gatttgattt ggcttactaa tccatgcaca gaatctggta
    33121 aatatcctat acttattggt tttggaccat ttttggttat tacactactc acttctaccg
    33181 cttgttcatc tttaaaatct cttatagaac cattgtttaa tgtattaact acttggatat
    33241 cttttttgtc attgtatata gaacttatta aattacaagc agcatcacta tagtaagccc
    33301 ctcctctttt ttcaagttga ggtggtttta catctaattt ttcatcctta tataattcaa
    33361 ataattgttc ttccaattct ttaactactt gacctctagc ttttccctct ttaaactctc
    33421 tcaactcatc ctcaagcatt tctttagttt tatagtagta tctatgatat ggacaaggta
    33481 ttgcacccaa agattttaca aattcagcat taaaatcaat tgcctttata ttttgcatac
    33541 ttatatctgc tttgacaaac ttatcaattg cttcttttgt aacatcttct ccatctaaag
    33601 atacatttag accataaacc atatgattaa gacctgcaaa atccatactt attctgtcac
    33661 tttctacatt aaatagtttg gcaacatcat tttttaaatg aattggcaca ttgcatagac
    33721 ctatatatct tctaaaatta gtatatttga atacagcctc tgttattatt cctgttggat
    33781 ttgtaaaatt caccatccat gcatttggac ataattcttc cacatctttt attatatcaa
    33841 atataactgg tattgtgcga agggctttaa ataaacctcc agcaccatta gtttcctgcc
    33901 ccataactcc atgagataaa ggaatagttt catcttttat tctcgcatct aaaagaccta
    33961 ctctaagttg tgttgtcaca aagtctgcat ctcttagagc ttctcttcta tctaaagtaa
    34021 gatttatttt catatctact ccagcttttt ttaccatcct cttagctaga tttccaacta
    34081 tctctaattt ttcttttcca gcttctatat ctacaagcca taattccttt acaggtaact
    34141 cttcatatct atttataaaa ccctctacaa gctcaggagt ataacttgaa cctcctccaa
    34201 ttgtaactat ttttagtccc tttttcatct cattatacca tccttaccta taatttaata
    34261 tactctacaa aaccctatac atttatttac cgaaataaaa ctacctagtt gttaattttc
    34321 aatttattag cttccatttt ttcagacgct atgacaaatg gaatatatat taatattgat
    34381 aatattaaat taaatagtgc taaagctcca ccagtccagt cacctgtagc catcgctcca
    34441 cctaatatag gaggtgttac ccaaggtatt ttaggtaaaa ttactggtgc tactaatcca
    34501 aaatctattg ctaagtatga tacagcacta catatcactg gtgcaagtac aaatggaatc
    34561 ataaatatag gatttaaaac tattggaagt ccgaatatca atggttctcc tatattaaat
    34621 attgcaggtg gcccacctaa tactatcatt tctttacgtc ttctcccagc tattaataaa
    34681 gctactatta atcccaatac cattccagaa ccacctaaat atacaaatgc atcaaagaat
    34741 gcacctgcaa gtacatggta tccttccgtt gctcctgctt gagccaaggc agcattttct
    34801 ccacctaatt tcataagtat agtttctgta aaaggaagtg ctatatttgc accatgaagt
    34861 ccaacagtcc aaagtatatg aattataaat gcaataagca ttgtagaacc tagactatca
    34921 gctacatttg atagcggtgc tcctaaataa gtatttaaaa tatctgttaa gaaacttcca
    34981 ttagaaagca tttttataaa tattcctatt acagtaaata tcattatagt taacattcct
    35041 ggtattaact ttgcaaatga tcttgaaact gctggtggaa ctccatctgg catttttata
    35101 gttaatttct ttaccttaga taatcttaca aatatctcag ttgataataa tcctattaat
    35161 atagaagaga ataaagctgc tgtacctaag taggtttgtt gtatcattcc ccatccttca
    35221 actactgtag ttcctccctc aggaactatt ttaccaattt gtggagccat tataaaaaag
    35281 atagataaag ctataagccc agattgtaac ccatcactct cataagattt tgctagaaaa
    35341 taagcaacac ctattactaa gaatactgcc attgtaccta ttgctcccca ccaaaggtcg
    35401 cctccaaatg tggtccaatt ttctccaaat atacttgcca tcaaattttt gtatgcttcc
    35461 aatggtaagt tatttatcaa tgttcccaag gcacctacca tagttatagg tatcattact
    35521 ataaatgcat ctcttactgc tactaagtgc ctttgagctc ctattttagc agctacaggc
    35581 actatatatt tatccataaa tgacatgaat ttttccattt tacatcctcc agtacaactt
    35641 tattttgaac tatttaactc tacagctcta tctaacacag cttttccatt acatcttcca
    35701 taatccatca tatttataac ttctactgga atattatatg gttttgccat ttcagttgct
    35761 tttcctaata catatctaac ttgaggccct aaaagtaaga catcacaatt ttctatttca
    35821 ttttttaagt tcacttctga tatagcccat atttttgctt ctataccgtt ttcctttgct
    35881 gcatcctcca tttttgtaac taataaacta gttgacattc ctgctgaaca agctaacatt
    35941 atttttatca ttttttcccc tccaaattag attcattaaa tattttttaa gtcataaatt
    36001 ttttaaacta tacatttaaa atatgctttt ctattaattt tttcttaatg tatatatctt
    36061 atttttaaat gtatatacat ttaattcttg atattattat ataccaaatg tatatacatt
    36121 tcaagaaaac tttttcattt tatttaaatt ttattcgaca acagcattca aagttaatgt
    36181 atatccatag tgatatgtgt gtgagtactc aaaaatctca ctattttgta acttacaaat
    36241 ttggcttata tataaaagtg gctctggttc ttctaatttt agattatcca tacattcttc
    36301 attcgatatt acagcttgta cttctctctc tgacatggct atcttgtatc cacacacttc
    36361 ttcaacaaaa ttataaagag aattttcaca atgagtctta tttaaatttg gaaataactt
    36421 aactggcata taagtaatca tatatgaaac tcttgcatca ttggctaatc ttactctttt
    36481 tatttcccaa acattttctt ctaaacttat attcaactta cttgataaat tttcatctgc
    36541 ctgaactatt tcaagagata ccaatatatt agatacttta tagcctttac tttccatctc
    36601 tttagtaaat ccacttacag acgatatatt atcatatata ttatttgcaa taactatact
    36661 acctacacct ctttgtctag ttatatagcc ttctttaact agattattaa gtgcagacct
    36721 tacagtcatt ctagttacat taaattcttc actcagctgt ttttcagaag ggattaaatc
    36781 cccttctttc aaactgtctg aatttattaa ctctctaaca tgattttcta taactttata
    36841 aattggctct ttcatttaat tcactcctta ttaattaaat cttttatata ataagataat
    36901 tttatataca aataaagaaa atgtctatac atttaagata atttaaattt tattcagaca
    36961 tcttgtacaa ctttccgagt tttaataaag atataaatag acttatcatt aatatacctt
    37021 ttaatatact tgataaggtt atactccacc atacaccatt tagacccaac atttctggtc
    37081 tagatataaa gtaagccaat ggtactcttg caccagtcag tatagtaatt ataatagaag
    37141 gtatataggt tctgcctaat cctttgaaag ctcctgttgt tattatttct aaacacataa
    37201 aaaattgaga atagcctaat attttaagat aaattgctcc tttttctatt gcctcacttt
    37261 catctataaa aatagaaaat atactctctc ctgcaaatac taataccaaa gtagttatta
    37321 tcccaagtac agaagataca gttatagcta ttttgcaacc tttatttatt ctagaatact
    37381 tcttggcacc ataattttgt ccaacaaaac tgcctaatgc aactgatagc ccttcagccg
    37441 tcatccagga aattgcttct atttgtgagc ctactttttg tactgcaaca gccacaggcc
    37501 cccatgatgc tactattact cctaatagca ttgagaatac tgtaaacata ccactttgta
    37561 ttgctatagg taatcccaat ttatacagaa ctttataata tttagtctct atttctttaa
    37621 atagtcttaa tttaaaatat tcttctttac ttttaactat tatatatata aaacatgtag
    37681 taactactat ctgagcaaat acagttgcaa tagcagctcc tgcaacacca agttttggag
    37741 caaatcccca tccaaaaatt aatataggat caagtataat attagttatt aatcctactg
    37801 tattaatacg aaaaggagtt ttactatttc caagaccgtt gaaaattgca gtaaatactg
    37861 gattaataaa ataaaatacc atacctagag ctactattat caaatactgt ctagacatgg
    37921 ttataacttc taagtctcct aatctaaaaa aacctatcaa ttgtttatta agtactatta
    37981 aagaaattgt atatattatt gctagtataa tatttatttc tattgctgac ttcatatatg
    38041 atttagtagt gcttatatta tttgcaccaa tactttgtgc tacttttatc tctccaccta
    38101 cttttgaaat cataataaat gccattgcaa gccatggaaa aaaaccagct gttccaactg
    38161 cagctactgc tttacttccg gctttaccaa cccatatcat atctatcatg ttatacccca
    38221 tctgtataaa tgaagtcccc attattggaa gtgatagctt aagtaatttt tctgtaattc
    38281 ttccttctgt taaattaatt ctgtctttca ttttccacct cttttatatt aaatttattt
    38341 gcttatatag ctagattaat cattttattt gattgagttc actatccttc aatttatatt
    38401 atcatatctc aagattgcat ctcaaccata cttatgttta agataaagta ttttacctat
    38461 ctctgataaa attaatactt atgaatataa atatagtcat ttcatcaata catattataa
    38521 cccttaactt aacttcaatt aagtatacat actgtaatca attagaatat tttataatgt
    38581 atcttccatt aagaaataac aaatataagt gatttatcta taattttatt tatacataaa
    38641 atagtatact gcaataataa aaaaactata tttgaatact ttaagcatgt ttactaattg
    38701 taatttccac acaaatttga taatatatga atatattatg gtgaaattta tagatatata
    38761 ttaattatgg ggagattgtg ggaggttctt ttcacaagac atttaagcta tgaaaataaa
    38821 taaaaaattt aatagagttg atatgcaggt ttcaatactt actgcaatta ttgtaataat
    38881 atcttcctca tgtgtatatt tttttaatta tacattaact tataagagca tgatatactc
    38941 tttaagtgag aggtcaaaac atatctatga atatgtagag agaaatattg ataaaaatac
    39001 ctttactgaa attaattcta aagaagacca atcaaaaaag tcttataaaa gttctaaaat
    39061 cctattagaa tctgtaaaaa atactactgg agtcatgtat ctatatactg ctaaaaaaac
    39121 aaaagatgga tcactagtat atgttgttga tggtttaaat tcatctagaa gtgattttag
    39181 aaatgctggt gatttgatag aaaaagagat ttggggagat cttaacaaag cattagataa
    39241 tcatatattg ttacctaaaa agattaaatc aacatcttgg ggatacatat ttatatcgta
    39301 ttttcctatt cacaatgatg ttggtaaggt tgttggagta ataggaattg aatttgaagc
    39361 aaaacatcaa tacaatacat ttaaatttat aagtatagtt acaccaataa tagcattatt
    39421 aacttgtcta atatcagcat tgattgcagt gattctattt aaaagaatat ctaatcctac
    39481 atatactgat tttgctaata cagattattt aacaggccta aaaaatagaa atgcttttga
    39541 aatagatatg aataacttaa acaattcaaa tctaaagaat ctaatatcta tcatatcaat
    39601 agatttagac ggtttaaaaa agattaatga taaatttggc catcagacag gagacttata
    39661 tattaagcaa gcaagtaaaa ttatacaaga tgtaataaat aaaaaatata tagtttatcg
    39721 tattggaggt gatgaataca taatta
//
LOCUS       DAELXX010000023        39609 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.023, whole genome shotgun sequence.
ACCESSION   DAELXX010000023 DAELXX010000000
VERSION     DAELXX010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 39609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39609
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.023"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(128..979)
                     /locus_tag="KPH29_001159"
     CDS             complement(128..979)
                     /locus_tag="KPH29_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG1466783.1"
                     /translation="MNSKIKYFLKFFILIFILIFIYSVAIEPSLLIVKKYNISENGAL
                     IKNDTIESKSESVKVVQISDTQIGSFYSTKNLKKVANKINTLNPDIIVFTGDLIDYSN
                     KNPSVDEITTILSSMNARLGKFSVFGNHDYMYKLPRYYRQIMKNSNFNLLVNENKKIK
                     LKDDKYINILGVDEILNGNPNIKYLESQIDNKNFNLLLAHEPDLVDMLSKDTMNLVLS
                     GHSHGGQIRLPIKGALVTPPYGRKYTKGFYDINGNHLYVSSGLGSTKLPFRFFNIPEI
                     IEFNIII"
     gene            complement(1005..1943)
                     /locus_tag="KPH29_001160"
     CDS             complement(1005..1943)
                     /locus_tag="KPH29_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBG1466784.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            complement(2046..2696)
                     /locus_tag="KPH29_001161"
     CDS             complement(2046..2696)
                     /locus_tag="KPH29_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBG1466785.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIKEDLENLD
                     QFIGPPLHDTFKEYYKFEDKKAKEAVEKYREYFADKGIFENKIYENMKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEELSESEPTYIVENVESIKDILL"
     gene            complement(2846..3034)
                     /locus_tag="KPH29_001162"
     CDS             complement(2846..3034)
                     /locus_tag="KPH29_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBG1466786.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            complement(3141..4325)
                     /locus_tag="KPH29_001163"
     CDS             complement(3141..4325)
                     /locus_tag="KPH29_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG1466787.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPSNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTSLDVINFDKVESIVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGVDQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLNVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            complement(4467..5384)
                     /locus_tag="KPH29_001164"
     CDS             complement(4467..5384)
                     /locus_tag="KPH29_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HBG1466788.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTEAGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            complement(5406..6134)
                     /locus_tag="KPH29_001165"
     CDS             complement(5406..6134)
                     /locus_tag="KPH29_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG1466789.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            complement(6330..6530)
                     /locus_tag="KPH29_001166"
     CDS             complement(6330..6530)
                     /locus_tag="KPH29_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466790.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            complement(6655..7035)
                     /locus_tag="KPH29_001167"
     CDS             complement(6655..7035)
                     /locus_tag="KPH29_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG1466791.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMKQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            complement(7308..8666)
                     /locus_tag="KPH29_001168"
     CDS             complement(7308..8666)
                     /locus_tag="KPH29_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG1466792.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTIMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDSEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGILRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAISIAIARIIGCIVIIRAL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     VA"
     gene            9114..9566
                     /locus_tag="KPH29_001169"
     CDS             9114..9566
                     /locus_tag="KPH29_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staygreen family protein"
                     /protein_id="HBG1466793.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRVFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            complement(9723..10340)
                     /locus_tag="KPH29_001170"
     CDS             complement(9723..10340)
                     /locus_tag="KPH29_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HBG1466794.1"
                     /translation="MEIFAIGHSNYPYDKLINMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFIYIYMGKEFGAKRNNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDREQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
     gene            10889..13549
                     /locus_tag="KPH29_001171"
     CDS             10889..13549
                     /locus_tag="KPH29_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HBG1466795.1"
                     /translation="MKKILKRLCTGFLAFATVVTALPSSTVHASNTQYWTESKERVSI
                     VEQVMNDGSISSTFNEGHLTVEGEDAYCIDINTAFKNGYKTKADASTRMSADQIADVA
                     LSIEYVKQYTDIHSGISSKHAYLLRQLVVWQRLSVQPDWQWDNVRASYDEIPKAVQDE
                     VFAGAKAFVKENKGRYDCGGYIYSGEGQELGQFWAKLAVGNTKLQKTSTNANITDGNG
                     IYSIAGATYGVYSDKDCTKQLTTLTTDTSGNTEAVEVRATIVYIKELSAPAGFKIDKT
                     VYSLSVEVGKTATLKVSDTPKVTDTLIELFKIDMETQKSNPQGNASLEGAEFTWNFYA
                     GYYNKNNLPAQPTRTWVTKTIAEKDSDGAIHYITRLADKYKVSWDSFYTQDGKNVLPL
                     GTLTVEETKSPSGYLLEGTYMQADGSEEQIKGMYLTQITEDGDLAVLSGSNQYHVSDK
                     VIRGGVKIQKRDLETKDTKAQGGATLKDTAFEIISLNENAVLVEGKLYKKNEVLKTIH
                     TDIEGIASTSADLLPYGKYRLSEQKPPEGYLTDGAKPIDFEITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGSHKRLADVPFRITSKTTGESHVVVTDDNGQFSTASDWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALLYDTYIIEELRCESNKGFKLIPPFEIVISRN
                     KVVVDLGTLTDEYEKEITIHTTATSKDGEKTILAGKDVTITDTVKLDGLTKGTKYQLK
                     GWQMLKEENAELIINDKRIENDYIFTADSETMEVKIEFTFDASNLGGKQLVTFEELYD
                     LSNPDEPIKVAEHKDIEDDGQTVTIKEVPETPTPEEPEKPTTPDTPTKTDSPKTGDNT
                     NILAFAIMMFVSAGGLAGTYFFKHRKMKKS"
     gene            13774..14097
                     /locus_tag="KPH29_001172"
     CDS             13774..14097
                     /locus_tag="KPH29_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG1466796.1"
                     /translation="MELKYIVPNVAQTFGNLEFAGENKVDQQRINGRYTLVSRSYNLY
                     SDIQRADDIIVVLPAKAGEKHFKPEQKVKLINPRITAEGYNVKGRGFTNYILMADDML
                     PAESK"
     gene            14112..14495
                     /locus_tag="KPH29_001173"
     CDS             14112..14495
                     /locus_tag="KPH29_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG1466797.1"
                     /translation="MRLTNGIVLDNEKTFGVLKFSALRHEVRVENEDGTTSDEIKRRT
                     YDLKCSVQERMIQVSIPADVPVKDFPYNAEVELINPVADTVANANFRGTTVDWYIKAD
                     DIVLKNKGGQAGNPQNNAPQGQQKK"
     gene            14591..15004
                     /locus_tag="KPH29_001174"
     CDS             14591..15004
                     /locus_tag="KPH29_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBG1466798.1"
                     /translation="MLDKIPNAEEMTALVGQSLYSVWNELCALIDEKYEMECLWNKGG
                     KAWNYEYKYRRGGKTLCALYARENCVGFMIILGKDERLKFDTYRDNYSKEVQRIYDET
                     KTYHDGKWLMFEPTDTTLFDDFVKLLRIKRKPNRK"
     gene            15111..15569
                     /locus_tag="KPH29_001175"
     CDS             15111..15569
                     /locus_tag="KPH29_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG1466799.1"
                     /translation="MKLKNPMLVVTDIDKSVEFYKKVFGLYVIMDFGANKTLTGGLAL
                     QTSETYKEFIGTSDISFGGNNFEVYFEEEDFDKFADRLKEYDIEYVHPIIEHSWGQRV
                     VRFYDPDKHIIEVGENMKIVCKRFLNSGMTPEQVAERMDVPMKFINACMR"
     gene            15594..16136
                     /locus_tag="KPH29_001176"
     CDS             15594..16136
                     /locus_tag="KPH29_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG1466800.1"
                     /translation="MKGFERKNQLFSLCGLNCGLCPMLLGNYCGGCGNGNQSCRIAKC
                     SLEHGQIEYCYECKQYPCEKYQHIDDYDSFITHKRRKADLERAKNIGIEQYNLEQQEK
                     TQILSYLLSNYNDGRRKTFFCVAVNLLELSELQEAMKQIQSNDELTLLPFKAQCLYVV
                     EVFQKIAERRNIKLKLFKKK"
     gene            16172..16699
                     /locus_tag="KPH29_001177"
     CDS             16172..16699
                     /locus_tag="KPH29_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HBG1466801.1"
                     /translation="MTNGGIPLETVELRFQYTQSEYIKAERQYLISSKTIHKYDIALV
                     AVFLLLSVVYMLFSSFSVFSILIFGLIIVVTALGSYLYILMPILKFKQTAKYHEEYTL
                     VFSKENIKFKTQSIDSEVKWDIYSALWESHDFYYLIQAPRLYTLIPKRIFKDLNEKQL
                     FEEIAQSRVKTTKHI"
     gene            16809..18203
                     /locus_tag="KPH29_001178"
     CDS             16809..18203
                     /locus_tag="KPH29_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG1466802.1"
                     /translation="MRMILNKGHRIRARDKNLVYHFSIGTLLFVFVAVLLLLNIKQLM
                     RTDWEHFSLLDNGLTLSPYNFITILIATGICALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIVWFPKIYYQMENGLLHIHCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRISIDEVKAENGCLRLMKNLV
                     WEYDVLPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEDMIDCVNAFYEGMVQRSEEMKQHPDYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGSLAFVRQ
                     DGTATCEAKGDGTD"
     gene            18483..19604
                     /locus_tag="KPH29_001179"
     CDS             18483..19604
                     /locus_tag="KPH29_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBG1466803.1"
                     /translation="MAGISREHLSRIEAGKVALTEDMKHKLLEAVEKFNPDAPMFLLF
                     DYVRIRFPTLDIQHVIKDILKLNIDYMLHEDFGHYKYTEHYYIGDVFVYTSQDKEKGV
                     LLELKGKGCRQFESYLLAQERSWYDFLMDALVDGGVMKRIDLAINDRTGLLDIPELIE
                     KCKNEECISKFRSFKNYGSGELVKHNEANKDGMGHTLYIGSFTSEVYFCVYEKNYEQS
                     SKLGIPIEEVPIKNRFEIRLKNEHAYYAIRELLTHYDAELTAFSIINQYIRFADKEAN
                     KRKSDWKTNARWSWFIGEGRPPVKLTTKPEPYTLQRTLNWLQRQVAPTLKMLKQIDAG
                     NQMDYLKTIEQQAKLTDKHKQIIRQQTASVDELIEKEKE"
     gene            19611..19745
                     /locus_tag="KPH29_001180"
     CDS             19611..19745
                     /locus_tag="KPH29_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBG1466804.1"
                     /translation="MWELIKDLLLVSLGMGIGVVLMCIMSVGKEADRHMIELKESEEK
                     "
     gene            19746..19967
                     /locus_tag="KPH29_001181"
     CDS             19746..19967
                     /locus_tag="KPH29_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466805.1"
                     /translation="MNFGQNLYTWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALVAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            20287..20925
                     /locus_tag="KPH29_001182"
     CDS             20287..20925
                     /locus_tag="KPH29_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase CAT"
                     /protein_id="HBG1466806.1"
                     /translation="MEYKYTKIDLNKWNRGKLFQSYIDNMRIVMSLTVDIDVTKFIEF
                     SKKHDLKFYPAMIWAVSKVVNEHDEFKYSWDNNGNLIKWDYISPSYTEFHKEDENFTK
                     IVTDFSDSIFEFHKRFILDREKYKTERAFVENQSLNFFDVSCLPWVKYNHFDVHVFDE
                     GKFLAPVITWGKYELEQERYIMPLTMNIHHAVADGFHLSRFFNEVQELINSI"
     gene            21002..21190
                     /locus_tag="KPH29_001183"
     CDS             21002..21190
                     /locus_tag="KPH29_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466807.1"
                     /translation="MKDIFSDMQASIGCTYISDLPHHKRMVWQEMKRLNLADYPQKQL
                     EDFAGYVFGVPYTSIAEA"
     gene            21211..21708
                     /locus_tag="KPH29_001184"
     CDS             21211..21708
                     /locus_tag="KPH29_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG1466808.1"
                     /translation="MNEMRVCIESRLHTSEEIRSAWFTLPIDEEELEEQIGVPIDSED
                     YHIVEKELPFADEVGEDATIEKLNDLYHTFESLPADLQEDYGELMCYFTSLDELHQHR
                     HDVIHYSWCKNMADVARHLLDNDPAFSAIEERLTRYFDYEAYGQYLDDNGEFIETDHG
                     IYELP"
     gene            21724..22227
                     /locus_tag="KPH29_001185"
     CDS             21724..22227
                     /locus_tag="KPH29_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG1466809.1"
                     /translation="MVEDMRVYIANLGKYNEGYLVGDWFSFPIDEEDVAERIGLNERY
                     EEYAVHDTENFPIEIGEYISIEELNEMYEMICELPDYITDSLDEFVSNYGSLEELYDH
                     RDDMILYAGCEDMTDVAYYFIDELQALGDIPPSLQNYIDYEAYGRDLSFEGTFIETRY
                     GMCEIPW"
     gene            22340..22729
                     /locus_tag="KPH29_001186"
     CDS             22340..22729
                     /locus_tag="KPH29_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG1466810.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDLPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITFALRPKITY
                     AGKAVKLDKQVLHEAITAVRSVSYVPD"
     gene            22716..25148
                     /locus_tag="KPH29_001187"
     CDS             22716..25148
                     /locus_tag="KPH29_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG1466811.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSSRSIQEQSKKLVTGRLKDVAYQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTDQSMNLKSVKQSAFLTFKEFIHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLDKNDFGYLIEHIYGRDGVAYEDYEYSLPKKKLKKE
                     TLITHYDLIRPTRCLVEESQRHLRLEHEDSESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALSTVRNKKKELKDLDNHAYQSGSETSSNVVDALDSVD
                     ELETDLDQSRESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYIGYSVDTGRNVYLQPS
                     LASQGVKDTVTNALASAFVESLGGGKSFCNNLLVYYSVLFGGQAVILDPKSERGNWKE
                     TLPEIAHEINIVNLTSDRENAGLLDPFVIMQNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRAVTQSDQRGLLHVIEELRKEDTPISCNIADHIDSFTDYDFAHLLFSDGTV
                     QNAISLDNQLNIIQVADLVLPDKDTTFELLSVSMLIVISTFALDFIHSDRSVFKIVDL
                     DEAWAFLNVAQGETLSNKLVRAGRAMNAGVYFVTQSSGDVSKESLKNNIGLKFAFRST
                     DINEIKQTLEFFSIDKEDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFEELLHAF
                     DTRPPIQRNEVE"
     gene            25148..27283
                     /locus_tag="KPH29_001188"
     CDS             25148..27283
                     /locus_tag="KPH29_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG1466812.1"
                     /translation="MKERIKSVFTRKKIFRFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINTENLYSKYPLSNYQLDFYADNNWAWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     RLYLSNATGYVVQEAYKLDFINDMADSIGRSIQTLAGVTQNGFSTSGFYVGFLLLIIL
                     VVGIYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKVNDFSSDISTAS
                     LDLGTKIMLPDSDSKGKDSVDLIRDSLFSIQVQQPWLLLQFGNSGIEEIGADRVEALV
                     SASPEDEDGKTREEVVKTEIEDNDNNNLTILQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVYFAGIYMKLGDLMSMFSLNAGDSQSMGRRIFRRP
                     YLFMRHRARRMEHRLARAVSAGGVAGAVAGGVVASSKSTRQNGTKNNRGNTSSSMGQK
                     AGSAVGAVLDTKNKVKDKATAVKENIKDMPTQTAYAVHSAKEKAKSSVSDFKRGVVQE
                     KENRQSGRLKKQQLHRQNIADKRMELQKAQESRQAGKKTDGSATSGATRPHERPATTS
                     KVNVEKQQEVKRPAIASTVKNDEPIKKNVVKERPLSSGDTSAKTVNQETTRQNYKTDR
                     VTKTTVQKHTQQNTEKNKRTVFKKGQKKK"
     gene            27280..28287
                     /locus_tag="KPH29_001189"
     CDS             27280..28287
                     /locus_tag="KPH29_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBG1466813.1"
                     /translation="MKLRHIAIVGSLFSILFSLVLFFGVLISAEDDDGGNGYSSNYVG
                     MNLSAEVLKHQPMVEKYAKENGISEYVNVLLVIIQVESGGTAEDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSCKNQGISDINVVIQSYNYGGGYVGYVAGNGKKHMFNLA
                     ENFARDKSGGQKVTYTNPIAVAKNGGWRYGYGNMFYVELVNQYLVVNQVSGELAQKVM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLVQWCYNKAGVSLPRTAQNQYDATQHIPLSQA
                     KAGDLVFFHSTYNAGSYVTHVGIYVGNTQMYHAGDPIGYADLNNAYWQQYLICAGRIK
                     Q"
     gene            28303..29196
                     /locus_tag="KPH29_001190"
     CDS             28303..29196
                     /locus_tag="KPH29_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG1466814.1"
                     /translation="MFKKKEKPVKETKVRTMKVGTHKKSVIALWVVLIASVSFGVYKN
                     FTAIDMHTVHETETIQLRLNDTNGVENFVKNFCKSYYTWDNSKEAIEARTQAISNYLT
                     KELQDLNLDTIRTDIPTSSTVTNVLIWDIKQSGTNDFIATYEVDQQIKEGEQTSNVKA
                     TYTVKVHVDKDGDMVIVQNPTIAPAIEKSDYEPKAQEADASVDADTVNDATAFLETFF
                     KLYPTATEKELAYYVAGNVLEPIGRDYLYSELVNPMFTDDRDNVKVRVAVKFIDNQTK
                     AMQVSQFELVLHKDSNWKIIM"
     gene            29409..30266
                     /locus_tag="KPH29_001191"
     CDS             29409..30266
                     /locus_tag="KPH29_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG1466815.1"
                     /translation="MYKEVGISSNNVPITLSIWYTERNSPTIVFLPGTMSHPLMYENF
                     LCGLSERGFNIIGIHYLSHGKSPKIKKNYTMEDLLHNVYDATTYGIENFGENIAVMGS
                     SQGGILAAMAAGRDTRLKAVFPHNIMLTTLKETMSLTKYPAWSHRFMGLIQLGFRVGG
                     KLLPNYKVPGDAYLDYDRVFYSEQAKQDCLNDPMLLPYYPLRFVASLFNADLSCLENG
                     AIQCPVILITAKGDPLFRFDYTNKVFDLIHVPKKELLTLELNRHMIFNEDTDTTINAL
                     EATLHKYLG"
     gene            30344..30925
                     /locus_tag="KPH29_001192"
     CDS             30344..30925
                     /locus_tag="KPH29_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG1466816.1"
                     /translation="MPRFTEQEKEIINSKLLIEGEKLFSLYGLKKVTVDDLAAAVNIS
                     KGSFYAFYPSKEHLYVEINFRLQKELFTSIETTVKKKKYKNHRDLAKDVIMLSLTGVI
                     TSPILSQIDLSLMDYLQRKISSDIFENHMYSDIRILEILEGLGVVFLVPHTVIIKSLY
                     SVLSCLEQFKEDEELNMVQNLLVNGIIQQVVAE"
     gene            31029..31874
                     /locus_tag="KPH29_001193"
     CDS             31029..31874
                     /locus_tag="KPH29_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1466817.1"
                     /translation="MIDVKKLYFSYTDKPFVENVSFHVGRGEIFGFLGPSGAGKSTIQ
                     KILTGLNTSYKGSVKVAGTEIREHTNRFYENIGVDFEFSTCYEKFTARQNLAYFASLY
                     DKQPRSIDELLHMVGLENDGDKKVADFSKGMRSRLNFIKALVHDPDILFLDEPTSGLD
                     PTNNRLMKDIILAEKKRGKTIIITTHNMYDATELCDQVAFIVAGKVSALDSPHNLIMS
                     RGAAKIHYTYYDKGEKTGECLLDRTTEDKLLKSLISENRLLSIHSSEPTLNDIFVDIT
                     GRTLQ"
     gene            31871..32554
                     /locus_tag="KPH29_001194"
     CDS             31871..32554
                     /locus_tag="KPH29_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1466818.1"
                     /translation="MRLGRLICGDIHFQWKYGFYFIYFILTVLYVCGIAALTGHWKTD
                     IASIMIYSDPAAMGLFFMGAIVLLEKSQKVLNAMVVSPVKVSEYILSKTIALIAISTI
                     IAVILGFVSGSNHLLGIAVGTALTSAIFTMLGIIAATKISNLNQFLIVIMPIEIVCFV
                     PPIVGLFVKLPDIFRFFPFTACMNLITGKSILLSFDMVLVIATLIILYIVARHTVKHM
                     WRSLGGVKL"
     gene            32551..33249
                     /locus_tag="KPH29_001195"
     CDS             32551..33249
                     /locus_tag="KPH29_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1466819.1"
                     /translation="MKRILSSTIQVFKQIKSDPMMFAACFTPFVMGALIKFGIPFLDG
                     ITDFSLQTYYPIFDLLLSIMAPVLLCFAFAMITLEEIDDKVSRYFSITPLGKSGYLFT
                     RLGVPSIISAVIAFIVLLLFSLEKLSIGMTICLALLGSVQAIIVSLMIITLSSNKLEG
                     MAVTKLAALTLLGIPAPFFIDSYYQFAVGFLPSFWVAKAMQNEAVLYFSIGLMVALVW
                     YYFLIKRLFRKLAG"
     gene            33261..34172
                     /locus_tag="KPH29_001196"
     CDS             33261..34172
                     /locus_tag="KPH29_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG1466820.1"
                     /translation="MEKGRLLQNENINKAIQVSKITLIINLGLSLLKFAAGYIGKSSA
                     MLSDAVHSASDVLSTIVVMVGIKISEKQPDKEHPYGHERMECVASIILSVALAITGAG
                     IGYSGIKKIFSGQYNTLSVSSGIALTAAVLSIVIKEWMYWYTRSAAKHTNSDALMADA
                     WHHRSDALSSVGSLIGILGARLGYAILDPIASIVICGCILKAALDIFKESINKMVDHS
                     CDNATETKIREVVLQQQGVDGIDELKTRMFGAKMYVDIEILADGNLALYDAHRIAEGV
                     HQTIENNFPQCKHCMVHVNTNELPYSP"
     gene            34225..34365
                     /locus_tag="KPH29_001197"
     CDS             34225..34365
                     /locus_tag="KPH29_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466821.1"
                     /translation="MEQYWWIIIVMVLIVVGLLKIVIMKKYNQRRNSNNRSKNSEDDK
                     MY"
     gene            34480..34671
                     /locus_tag="KPH29_001198"
     CDS             34480..34671
                     /locus_tag="KPH29_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HBG1466822.1"
                     /translation="MKKYEYVEINYNAKGVVFLCTDKHKEIIDSYAKTGYHYAGFVPT
                     EIDAKGCMRKIDLIFEKED"
     gene            34695..34925
                     /locus_tag="KPH29_001199"
     CDS             34695..34925
                     /locus_tag="KPH29_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466823.1"
                     /translation="MIGLKRRDMNYIMKVGAKAQGVSVSEFRAMLQDTIDKTMESTDP
                     DVQANFKRYFGNKRPTPEEYIYTITKKTKVKV"
     gene            35191..35481
                     /locus_tag="KPH29_001200"
     CDS             35191..35481
                     /locus_tag="KPH29_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466824.1"
                     /translation="MKPSDFQKTIQCQFECKLKKVVKGIIWNYRKELRRRKNKEISFS
                     ELPDITVESLATWDDYETNYTIFNVCGIDIKVLDDELAEALKQLPEKNVTMY"
     gene            35481..35615
                     /locus_tag="KPH29_001201"
     CDS             35481..35615
                     /locus_tag="KPH29_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG1466825.1"
                     /translation="MYYFLEMSDTEIADIMNYTREAVFKHRHRALETMKEILNEKTRQ
                     "
     gene            35596..36777
                     /locus_tag="KPH29_001202"
     CDS             35596..36777
                     /locus_tag="KPH29_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG1466826.1"
                     /translation="MKRQDSKGRNLRLGESQRKDGRYVYKYTDIFGKPQFVYSWKLVP
                     TDKTPAGKRDDISLREKEKQIKKDLDDGIDTVGGKMTVCQLYEKKNNQRKNIKRNTEL
                     GRQYLVNALENDPLGMRAIDTVKQSDAKEWAIRMNGKGYSYKTIDNYKRSLKASFYMA
                     IQDDCIRKNPFNSKLSDVLEDDTEAKVILTPEQEEKLLAFMETDNVYSKYRDEVILLL
                     ETGLRISELCGLTTHIDMLNRVINIDHQLLRDTEVGYYISKAKTKNGKRELPLTERAY
                     QALKRILKNRGKAQPLVVGGYSNFLFLNREGLPKVAGNYESMVRGLIKKYNKTHKDKL
                     PNITPHSFRHTYCTNMANKGMNPNTLQYIMGHANITMTLGYYAHGTFQSAKAELERLA
                     S"
     gene            complement(37117..37929)
                     /locus_tag="KPH29_001203"
     CDS             complement(37117..37929)
                     /locus_tag="KPH29_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG1466827.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDAVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRIL"
     gene            complement(38005..39240)
                     /locus_tag="KPH29_001204"
     CDS             complement(38005..39240)
                     /locus_tag="KPH29_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBG1466828.1"
                     /translation="MGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITSGVTGMF
                     CHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLRGSLASLLEVESGSNDPIAY
                     MLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFYTIF
                     ITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIMLFF
                     MLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVGLRG
                     AASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTPVLK
                     TFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGSTII
                     KTGDILVLSGDRIEELIQL"
     gene            complement(39388..>39609)
                     /locus_tag="KPH29_001205"
     CDS             complement(39388..>39609)
                     /locus_tag="KPH29_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG1466829.1"
                     /translation="YKANWHGRQIVKVGKFFASSQICNKCGYKNEEVKNLNIREWICP
                     SCNETHDRDINASINILKEGLRLITIQNK"
BASE COUNT        12852 a         7068 c         7072 g        12617 t
ORIGIN      
        1 ataacttaaa tattttatat attaatttaa tatattaatt cattcaatct actggtttaa
       61 tccattttta cagtatatac atctacatat tataaaaagc aaaattatat tttctaaata
      121 ttttatgtta gattataata ttaaattcta ttatctctgg tatattaaaa aatctaaatg
      181 gtaactttgt acttccaaga cccgaactca catataaatg atttccattt atatcataaa
      241 accctttagt atatttcctc ccatatggag gagtaactaa tgctccctta ataggtaatc
      301 ttatttgacc accatgacta tgcccagata gaactaaatt cattgtatct ttactaagca
      361 tatctactaa atctggttca tgtgctaaca ataaattaaa atttttatta tctatctgac
      421 tctccagata ttttatatta ggatttccat ttaatatttc atcaacacct aatatattaa
      481 tatatttatc atcttttaat tttatttttt tattttcatt tactaaaaga ttgaagttgg
      541 aatttttcat aatctgccta taatacctag gaagtttata catataatca tgattgccaa
      601 atactgaaaa ttttcccaac ctagcattca tacttgataa aatagttgta atttcatcta
      661 cacttggatt tttattggag taatcaatta aatcaccagt aaatactatt atatctggat
      721 tcaaagtgtt aatcttatta gctacttttt tgagattctt agttgaataa aaactaccta
      781 tttgagtatc tgaaatttga actactttta cactttcaga tttactctca attgtatcat
      841 tttttatcaa tgctccattt tcacttatat tatatttttt cacaattaat agactcggct
      901 ctatcgcaac tgagtatata aaaatcaaaa taaatattaa gataaaaaat tttaaaaaat
      961 acttaatttt tgagttcaaa atatctacct acttatttat ttttttaaac tatttgcatt
     1021 acagactcaa ctttgttcat ttttctgtca tgcatttttg gcttatttgc tgaaaaccat
     1081 ttatatgttg ctgtaagacc ttcttttagt aacacatttg gtatataaag cccatgttca
     1141 ataagcttat ttatatcaag attaaagtct atatttctaa atgggaaata cgttctttct
     1201 ctaaactcta ctttttccat atctacatat tttattatag gttctttgcc aataatttct
     1261 ccacatgtat atataaggtc atcccaagaa attaactgtg gatttgtcac attataggcc
     1321 tctcttacat gaggattttt cattatgctt tccaatacct ttaccaaatc cccaatatat
     1381 ataaattgat tactaacttg ttttcctttt ggtactggta tcttttcatt tttttctatt
     1441 ttttcaaaga aataatactc tctatataaa ttattatttt caccatatat gtaagttggt
     1501 ctaataatta tatatggtat agggctattt ataataaaat cttcagcctc ttttttattt
     1561 aacccatatt ttccccagtt ttcattttca cctttctcat tttcttcttt tatatttctt
     1621 ccactatctt tatatactgc tcctgctgac aatactatat acttttttaa gttatccatg
     1681 cttataaaat caatcagatt acttacatct tcttttgtat atgctgtcat atcatatatg
     1741 taatcatatt ttcttcctgt gataatgttt tccatatctt ttcgaacttt tctatcacaa
     1801 attagatgtt ctttaaaacc attataatct atttcctttt taccatttgt aagtatatca
     1861 atttgatacc cacatttaat caagcgctta gcaagtgcag aacctatgaa gtcgcttcca
     1921 cccataatta aaatagattt cattttaagt gcatcctcct cttttttaaa tgacttcctt
     1981 aactataata tcatattttt aaatatgcga caccattttg taatattttt gtctataaaa
     2041 gatatttata acaaaatatc ttttatagac tctacatttt cgacaatata agtaggttca
     2101 ctttcactaa gctcttcaaa agaaccatac ccatacaata ctccaataga atctatacct
     2161 atttttttag ctccaattat gtcatacttt ctatctccaa ccataataac tttatcttta
     2221 tcttttacat tacataggtc tagtacatat tgtataactt catttttatt cactcttgta
     2281 ccatcaagat tgctacctgc tatatattta aaatatctat ctatatcaaa atatctaagt
     2341 atagtttctg caaaaactgt tggcttagaa gttgctacta ataatatttt accattttta
     2401 tagagcattt ctaatatctc tttcatattc tcataaattt tattttcaaa tatcccttta
     2461 tcagcaaaat attctctata cttttcaaca gcttctttag cttttttatc ttcaaattta
     2521 taatactcct tgaaagtatc atgtaatggt ggtcctataa attggtctaa gttttccaaa
     2581 tcttctttta tgccaaagct atttaaagaa tactgaatag actttgttat tccttcttta
     2641 gggtctgtaa gtgttccatc taaatcgaat aatactattt cataattttt tttcatattt
     2701 cagtcctcca tatttgtaaa aagtaatatc ttacatatta agtttatcta ttttaaaaag
     2761 ctatagtaat agaaatttct aatactatag cttaagtacc caattaaatc ttatatattt
     2821 attttaaaaa taaatttaaa cagctctatt taactgttat agctgaaact ggacatcctt
     2881 cttcggcttc tttacataaa tcttgtaaat ctgctggtat ttctccttca attgctactg
     2941 aatgcccttc atcgctcata ctaaaaacgt ctggacatgt tcctatacat gctccacaag
     3001 ctacacatac atccttatca acaaatgctt tcatactaaa atacctcccc taaaattatt
     3061 attttttaat tgttgtttcc ttagatattt atccaatata taccaattta aacatatatt
     3121 tattatttta tcactattgt ttagtttatt atagtatttt cgtatccatg tctataaact
     3181 tcactgcttt tcatccatga ttttacagta tattgcaatc ttaatttttt atttaaaaca
     3241 agttcattat tattgtagtc ttttataata tcattacact cctccattgt gaatattgta
     3301 aaatcacctt ctcctccaat ctttattctg cctttatttc catgtaaatc gaaaatcttt
     3361 gttgggtatt cagttacttt ttctatacac ttttctaggg atatcccaag ttccataatt
     3421 ttatttataa catttaaaag agaatagttt acgtctttca tgttttttat atgcatatca
     3481 gtacttatta ggtcaggctc taaaccttgc tttattgctt ttttaaaaac tttaaaatca
     3541 aattggtcca ctccatgaca tacatcgaac aatactcctc tattctttgc atttattact
     3601 gattttctta atgtgccaga ttctttaagg attccatttg tactatttcc atatactggt
     3661 gtaaccacat ctccttttcc caaaatattc attacttcat ctatatatga tggatactct
     3721 cctatatata ccactaaggg taaatttaat tttttagcaa cttgctttga tttcttaatt
     3781 atatctatac cactttcatt agattgaaat ttactcgctt ttacttttat accctttatc
     3841 ttttctatat ttttttctac aattgactca accttgtcaa aatttataac atctaaagaa
     3901 gttgattcat tcaatatatt tagtccctca tttgtaacat ttaaaaaaga atatacttct
     3961 gttttacact catccatata ctttttttta aaatcatagt agtttgaggg accagctgta
     4021 cctgcatcaa atataactgt agaacctttt tcaattccaa ttgaatcaga atccatacct
     4081 gtaggtaatt ttctcttata acaatgggtg tgaacatcta taaatcctgg ggaaatatac
     4141 atattatcgg catttattat ctcgaactct ttatttgtaa gtaatttttc ttttcttatt
     4201 ttctctttaa ggttcaatcc aatagcatat attttcccat gtaaaaccaa gatatctctt
     4261 ttttcaaatt tgataccatc tgttggagat attattgtcc catttcttat aagtaaattt
     4321 ttcatttatt ccacatccta attacaatat aatttctcta atagcttttc ttacataaag
     4381 aaaataagcc taaaaataaa aatattttag acttattcac tatattatac tatattaatt
     4441 atattaaatt aaataaaatc tttaatttac tttaagaagc aattaagtat ttgttgctcg
     4501 gcttcttctt cagtaagacc taatgtcatc atctttagta tttggtctcc tgcaatcttt
     4561 ccaatagctg cttcatgtac taaaagagca tctggatgat ttgcagcaat ttcaggtata
     4621 gcactaattt gagaatttcc cataattatt gaatcacatt gaacatgtgc agaacaaagg
     4681 gaatccccaa taacacaagg attaaacttt tgcttagaat tatcttgccc aactgtacga
     4741 gaaactactt gagcagaaga gttttcaccc tctaagataa ccttcatatt agattctgca
     4801 acttgattac catgagttaa taatttctca aatacaacta ttttagctcc tttacctagg
     4861 tttgcatcca tttctctttt agttgaatct acacccttaa tttgaattgt ttccatttca
     4921 caatatgaat cctcatccat attgacaata gtaactggat ttaaaattct ctttccttct
     4981 ccatcacctt caccataatg tttttctaca tattttaggc gagctccttt acgtaagaaa
     5041 aatctatgta taccattatg ttcactatca tcacaaccac agttatgtat accacaccca
     5101 gcaattataa caacatctgc attttctcca atttcaaaat cattatatac catatcctgt
     5161 acaccagcct ctgttactat tactggtata tgtacatttt ctccttttgt atttggcttt
     5221 actataatat ctattccagg tttatcagtt ttaggaatta tagttacatt ttcagttgaa
     5281 tgtctctcta ctccttgccc atttttacgt atattgtatg ctcctaatgg agttccacga
     5341 agttcagcaa ctttttctaa aagatttaaa tctattttgt tcatttaatt tgccttcctt
     5401 tctaattaat tcatgtcttt cattaattca caacttgaat taacttgaca catgatttca
     5461 ggtaatatag cctcttttgt tccatggctt tttactgaac catcttgtag aacaattatt
     5521 tcatcagcta actctaatat tctttcttgg tgagaaatta tgattatagt ctgattagac
     5581 tcttcatgta ttttcttaaa actttcattg agttttccaa agctccaaag gtctatccct
     5641 gcttcaggtt catcaaatat agaaaccttt aaatctctag caaaaagagt tgctatctca
     5701 attcttttca tttcccctcc agaaagagag ttatcaactt cacgatttaa atagtctttt
     5761 gaacacaatc caacatctgt aaggtattgg caacatacat cagtagacaa tggttttcca
     5821 tgagcaagtg taagtaaatt ttcaactgtc atacctttta ttcttggtgg ttgttgaaat
     5881 gcgtatccta ttcctttttt tgctctttct gtaatactaa gatttgttat atcttctcca
     5941 tctaaaatta tctgaccact cgtagctttt tcaattccca taataagctt agcaattgta
     6001 gattttcctc caccatttgg acctgtgata acaataagtt ttcccctctc aaatgaaagg
     6061 ctaacatcat ttattattcc tagttcctca ttgttagact caacattaaa ggaaagattt
     6121 tttatttcaa gcataatacc cctcttttct ataaaatatt acattaatat ccattctttt
     6181 attatatcag taatttttat atatcagcaa ctctaagcag ataaaatgta tattaaataa
     6241 taaaacaaag ttattgaaaa gtaaaaaaga tgagttttat gtaaactcat ctcagaaatt
     6301 tagtatatca acttatatgt atttaaaatt taaccttcta tatactctaa tattattttt
     6361 tgattatctt ttaacataat ttcttctttt ttagattcta ttccattctt ttttatcttt
     6421 acctcagcta caaaggcatc attaccttca accatttcta atagttcata cgctttttta
     6481 cgacctattt tagttgctat atcttttagt actgccattt tcttaaacat tttatcacct
     6541 ctactcaaaa tttacactta atttttaatt atataattct aatcctagac tagtttataa
     6601 taattattgt ctctaattat atcagatatt gagtaaattt aatactttgt aaaattattt
     6661 atcctctctg actaaataat catttccatc ttggtcttta aaagaaaaca tccttccata
     6721 tggcatctcc attaattccc caacttcaac ctcgttattt gccatttgtt cataagttcc
     6781 attaatatct cttgtactta aaattattga tggatgagat acatttatct ctttgttttg
     6841 cttcatcatt agatttttat catacaacac aaatgttgta aattcatttt tacttggtgc
     6901 tacttccacc catttcatat ttggacccat ttccttttca taagttacta caaagttcaa
     6961 tttttctgtc caaaacaact ttgcatcctc ttgattattt acatagagag ttatttttcc
     7021 tattttattt atcataacaa tacccacctt tattattaaa taaataccta atatttttta
     7081 tatttaataa tatacccata tatattattt tctatagata tatatttcta atatttatcg
     7141 ccaaattatg taattctgct caatatataa tttataatat ccatactatt atagccttta
     7201 tttatgtaat ttaataacct tatatatcat acttataatt atgataataa ctacataaaa
     7261 aatcacttct aaatagagtt atccatctaa aagtgatttc atatattcta tgcaactttg
     7321 tgttttagcc actttgttcc tcttaatctt aaacaatata aagtccctct aacaagccaa
     7381 tcagtataca tacctatcca aattcctaac actccaaatt ctaatacaac ccccaatacg
     7441 taacctgtaa aaattctaaa aatccacata cctattactg cagtcatcat tgtatatcta
     7501 gtatcaccgg ctccctttag ccctgctgat aatacaaagg atataggcca tattaccata
     7561 gctaaacaat cacttcgaat taattgtgtt gagatgtcaa ttacactagc attttgagta
     7621 tacatacttg aaatccaggg tgcaattggt ataaatatag ctcctactat aaataaacaa
     7681 attgttgcaa atttagttaa atataataaa gtactcttag ctcctttaat atctccacgt
     7741 cctatatact gtcccaccaa agttgtagca gacaaagcta aagcattccc aattacattt
     7801 attatggatg caattgacat tcctattgca ttggatgcaa ttgctgatgt tcccatagta
     7861 actataaata cttgaacaat caatttacct gcattaaaaa gtagttgttc aactccagct
     7921 ggtatcccta tattaaatat agacttttga gtttcaacat caatcttaaa tggaaatatt
     7981 ttacccatag atataaattc atttccttta aataaagctc ttataattac tatacatcca
     8041 attattctag ctatagcaat agatatagct gcacctgtta tgccatagga tggtgtttgc
     8101 aaatttattc ctagaggatt taaatcaata ccatatataa gtatatatcc taatgttaca
     8161 tttactatat tcataaacat agtcacatac ataggtttct ttacatctcc acaccctctt
     8221 aaaataccat ttgctatttg ttgtgtcgca ataaatggat acgtaaataa tgtaaactct
     8281 atgtataatt ttgcatttga ttttaccaat tcttctgctg aaccataaaa taaattaatt
     8341 acaccagttc taaaaaccca catcaatatt gttattgtaa aggataatat tactccagac
     8401 actagagcct gcttaatagt ttcattcacc ttttcactat ctttttttcc tatttgttga
     8461 gctacaacga cagtagctcc aacagataat gcagcaaata cagatataaa tatattgttt
     8521 attgaatcaa ccattccaac tgcagataca gcttcttccc caatatgacc tgccattatt
     8581 gtattaaata ctcctaaaag catgacaaac gtctgctcta tcattatagg aattgctaac
     8641 tttataacat ttttccttat aaacatccct attcctcttt tccctcttta tataaattta
     8701 atttttaatt agtttatcga actgtaaatt gacaatgcaa agtttagaat tgtattgttc
     8761 gcattataat atactgcttt caaataatat aaatcttgtt taatatcatt tgaacacagt
     8821 atatctttat aattatctta tgtttattat tttttatgga taccacattt aaaatattta
     8881 ttaatttaat ataaatattt tccgttatta ttaaaagtag ctatatttcg ttaatattgt
     8941 atcactatcg tgtagcaata acaataagta cattttatat attttttata atattattta
     9001 atcatccaaa cttttaccac tttttcaata tataaaatat tacactttag atgtaacaaa
     9061 atcgcatcca tttaaatatt gtattttata gactaatttt aggatgtgat actttggtta
     9121 atttaaatgc aaataatcta aatgtaaata tggtttatcc tatttcttcc acagcaccca
     9181 tacgtttcag aagatatact gtttcatatt tagaaaacat ggaaaatatt tatttaacta
     9241 tagcaacata ctttgataca cttggtttag gcacaatgaa tatcgacaaa gtttatggtc
     9301 aatgggcttg gtttactgat aatatatatg atttaaatct atttgttttt gtaggaaatt
     9361 atccttatca aattgctaaa aacagatatg atacatttgt agatattttg ccagtagcta
     9421 ttacttctat tgtcaatgga gatagagtat ttttaatttc aaatccttgt ttgttaaatt
     9481 caaaaattac agttagattt atatctagtc atcctactct taatagaaca gttgattatg
     9541 gcaatattag agattttatt ttttaaaact gcaacatgat gtgctattaa aaaataccct
     9601 aaatttaatg atattattaa ataactagaa tatttttaat tggcacatca tgcctagcta
     9661 ttataaattc ataatattat tttacttaag ataagtactg tattttatct acttgtatat
     9721 ttttatttac ctagtttctc agttctatat cctatttctt tattagctag cttataacct
     9781 tcttgtatca tttcttctct cgttaatgca ctccctaata aattgtcaat actcagttgc
     9841 tctctatcac taaaatactt atccaataat gattcctcta tatcttcctg atatgccaaa
     9901 tttccttcat gcattatata ttttacatcg aaaccttctt tatttaatac ttttcccacc
     9961 aaaatacttc tatgacatct tataggttct tgcatagctc ctaataaaac tatcctataa
    10021 cccatttgac aacccttttt taatctttct attccgttta aaaaaatatc ttcttttgct
    10081 actttctcaa aatctgcata accttcttgc gtatatacat ctttattatt tcttttagct
    10141 ccaaattctt ttcccatgta tatatatata aatccggaat ttctaagaga ttcgttaaat
    10201 gcctctttat tgtattgaat attatattta gaatatggag tttctctaat gtcaactaca
    10261 caatttatgt catatttttt tatcatatta ataagcttat catatggata atttgaatgt
    10321 cctattgcga atatttccat ataaataccc tcctattctt tgaaattagt atctatatat
    10381 gtagataccc gatttactta aaattaaata aagtattact ttaaataccc aatatctttt
    10441 tttcttgata ctgtatattt ttttacttta tatccttaaa aaaatataca actttatata
    10501 taaaacatca atatttatat atattttgta tattaaatac aattatttat aaaatctttg
    10561 ataaaaattt ataaatagag catctttaga aaactattaa cagaaaaaca cactaaaaat
    10621 ttgattatcc gctaaaacac tgtatctatc ggcattatca aggagctaaa cacatcaaaa
    10681 tactactaat ttactacgat tgaatgagcc ttgatacgtc ctatttccca gatatgcaaa
    10741 gatacggtac agcacatcag tattgaaaca agaaaaattg aatacttcat agactacgga
    10801 acgcacaata tcagcgttcc ttttctattt ccagccgttc ctagtggacg gctattttac
    10861 taccaaaatg aaaggagcat ttagaattat gaaaaagata ttaaaacgat tgtgtacagg
    10921 ctttcttgcc tttgcaactg tcgttaccgc tttaccgagt tcaacggttc atgcgtctaa
    10981 tacacagtat tggacggaaa gtaaagagcg tgtcagtatc gtagaacaag taatgaatga
    11041 cggttctatc agttctacct ttaatgaggg acatttaacc gttgagggtg aggacgctta
    11101 ttgtatcgac atcaacacag cctttaaaaa cggctataag accaaagctg acgcaagtac
    11161 ccgtatgagt gccgaccaga tagctgacgt cgccttatct attgagtatg tgaaacagta
    11221 cacagacatc cacagcggaa taagcagtaa acacgcctac ctattaagac agcttgttgt
    11281 ctggcagaga ttaagtgttc agcctgactg gcaatgggat aatgtgcgag cttcctatga
    11341 tgaaatccca aaagccgtac aagatgaagt attcgcagga gcaaaagcct ttgtgaaaga
    11401 aaacaaagga cgttatgact gtggcggata tatctactct ggcgagggtc aagagttagg
    11461 gcaattctgg gcgaagttag ctgtcggaaa taccaaactt caaaagactt ccaccaatgc
    11521 caatattaca gacggcaacg ggatttattc aatcgctggt gcaacatacg gtgtgtattc
    11581 tgataaagac tgcacgaaac agcttaccac ccttacgact gatactagcg gaaatacaga
    11641 agccgttgaa gtaagagcca ctatagtata tatcaaggaa ctttctgcac cagcaggatt
    11701 taaaattgat aaaacagttt actctttatc tgtggaagtt ggaaaaacag cgactttaaa
    11761 agtatctgat acaccaaaag taacagatac cttgattgag ctattcaaga ttgacatgga
    11821 aacacagaaa tccaatccgc agggaaacgc ttctttagag ggagcagagt ttacatggaa
    11881 cttctacgct ggatattaca acaaaaacaa tcttccagca cagcctacaa gaacatgggt
    11941 aacaaagact atcgctgaaa aagacagcga cggtgctatc cattacatta ctcgtttagc
    12001 tgacaaatat aaagtatctt gggacagctt ctatacgcag gacggtaaaa acgtacttcc
    12061 attaggaaca ctaactgttg aggaaacaaa aagtccgtcg ggttacttgt tagagggtac
    12121 atatatgcag gctgacggta gcgaagaaca gataaaagga atgtatctga cacagattac
    12181 agaggacggc gaccttgccg tattatctgg aagcaatcaa taccatgttt ctgataaagt
    12241 cattcgtggc ggtgtgaaaa tccagaaacg agatttagaa accaaagaca cgaaagctca
    12301 aggcggtgcg actttgaaag ataccgcctt tgaaatcatt tccttaaatg agaatgccgt
    12361 cttagttgag ggcaagttat acaagaaaaa tgaagtgtta aagacaatcc ataccgacat
    12421 tgagggtatc gcttctactt ctgctgacct attaccttac ggaaaataca gattgtctga
    12481 acaaaaaccg ccagagggat atttgactga cggtgcaaag ccgattgatt ttgaaatcac
    12541 agaaaatgga aaaatcgttg atttgactga cgaagcccac tctatctata accagattaa
    12601 acgtggcgat attgagggcg taaaaattgg tgcaggttcg cacaagcgtc ttgcagatgt
    12661 tccgtttagg atcacaagca agacgacggg cgaaagtcat gttgttgtaa ctgatgataa
    12721 cggacaattc tctactgctt ctgattgggc ttcccacaag cacaatacca atgcaggaaa
    12781 aacaagcgag gacggtgtgt ggtttggtac ttctgaacca gacgacagca aaggtgcgtt
    12841 gctttatgat acttacatca ttgaagaatt acgttgtgaa agcaacaaag gtttcaaact
    12901 tattccaccg tttgagattg tcatttccag aaataaggtt gtggttgatt taggtacgct
    12961 gactgatgaa tacgaaaaag aaatcactat ccatactact gctaccagca aggacggcga
    13021 aaagactatt cttgctggga aagacgtaac tatcactgat acagttaaat tagacggtct
    13081 taccaaaggg acaaaatatc agttgaaagg ctggcagatg ttaaaagaag aaaatgcaga
    13141 gcttatcatc aatgacaagc gtattgagaa tgactacatc tttacggctg acagcgaaac
    13201 tatggaagtt aaaatcgagt ttacctttga cgcttccaat cttggcggta aacagcttgt
    13261 aacttttgag gaattatacg atttaagcaa tccagacgaa cctatcaaag ttgcggaaca
    13321 caaggacatt gaggacgacg gacagacagt tactatcaag gaagtaccag aaacacctac
    13381 cccagaagaa ccagagaagc caaccacacc agatacacct acgaaaacag acagtcccaa
    13441 aacgggcgac aatacaaata tccttgcctt tgcaattatg atgtttgttt ctgctggtgg
    13501 gctggctggt acatactttt tcaaacaccg taagatgaag aaatcataag gcggtagctg
    13561 tatgagaaaa gaaaaatcac gctctccgcc atagcttaga taggcagact atacctattc
    13621 tggcttgtct gccgattacc tgcaagaaca cggatagtca agggtgcgtt agcatttatt
    13681 taccctttac tatctggggg cttgctggca ctttcctaaa tgccagaata cacacatcaa
    13741 tcaaacttaa agtaagaaac gaggtaaaat aatatggaat taaaatatat cgtgccgaat
    13801 gtggcacaga cttttggaaa cttggaattt gcaggggaaa acaaagtaga ccagcaacgt
    13861 atcaatgggc gttacactct cgtatcaaga agttacaact tgtattcgga tattcagaga
    13921 gccgacgaca ttatcgttgt attgcctgca aaagctggcg agaagcattt taagccagag
    13981 cagaaagtaa aactgattaa cccacgcatt accgcagagg gttacaacgt caaaggacgt
    14041 ggatttacaa actacatttt aatggctgat gatatgttac cagcagaaag caaataaggg
    14101 ggtatttgat tatgagatta acaaatggaa ttgtattaga taacgaaaaa acttttggag
    14161 tattgaaatt ctctgcactt cgacatgagg tgcgtgtaga aaatgaggac ggaacgacaa
    14221 gtgatgaaat taagcgtcgt acttatgatt tgaaatgtag tgtgcaggaa cgcatgatac
    14281 aagtgtcaat ccccgctgat gttcccgtaa aggactttcc ttataatgcg gaagtggagt
    14341 taatcaatcc cgtagctgat acggtggcaa atgccaactt tagagggact acggttgatt
    14401 ggtacatcaa agctgatgat attgtattga agaacaaagg cggtcaagct ggaaatccgc
    14461 agaacaacgc tccgcaggga cagcagaaaa agtaagctga caaactagtt tttcattgta
    14521 ggagatataa acaactgtta taatatggat gtggaaacag ttaatttaga cttataaagg
    14581 agaaatgtgt atgctagata aaatacctaa tgcagaggaa atgactgctt tggtcggaca
    14641 atcattatat agtgtatgga atgaattgtg tgccttgatt gatgaaaagt atgaaatgga
    14701 atgtctatgg aataaaggtg gcaaagcatg gaattatgaa tataagtatc gtcgaggtgg
    14761 caaaacatta tgtgccttat atgctagaga aaattgtgtg ggatttatga ttatcttagg
    14821 taaagatgaa cggttaaaat tcgatacata tagagataat tattcaaaag aagttcagag
    14881 aatttatgat gaaacaaaaa cttaccatga cggaaaatgg ctgatgtttg agccaacaga
    14941 tacaactttg tttgacgatt ttgttaaact tttgaggata aaaagaaaac caaatagaaa
    15001 gtagtttatt accacaccat tagcaaatgg atagtttctt ataatagcgg atatatgaaa
    15061 atgctgtcat gtggacataa agaaaattgg aagttgtgga ggtagcaaaa atgaaattaa
    15121 aaaaccctat gttggtagta actgatatag ataaatcagt tgagttttat aaaaaggttt
    15181 ttggacttta tgtcattatg gattttgggg caaataaaac tttaacaggt ggattagcct
    15241 tacagacaag tgaaacatac aaagagttta tcggtacaag tgatatttct tttggtggta
    15301 acaattttga agtctatttt gaagaagaag attttgataa atttgctgat aggctgaaag
    15361 aatatgatat tgaatatgtc caccctatta tagaacattc atggggacag cgtgtagtcc
    15421 gcttttatga cccagataaa catattatcg aagttggaga aaacatgaag atagtgtgca
    15481 agcgtttctt aaatagtgga atgacaccag aacaagttgc agaacgtatg gatgtaccta
    15541 tgaaatttat aaatgcttgt atgcgataaa ttgaaagtta ggagactaat actatgaaag
    15601 gatttgaacg caaaaatcaa ttattttctc tttgcggatt gaattgcgga ctatgcccta
    15661 tgcttttggg aaattactgt ggcggttgtg gcaatggtaa tcaatcatgc agaattgcaa
    15721 aatgtagtct tgaacatgga cagattgaat attgctatga atgcaaacaa tatccatgcg
    15781 aaaaatatca acacattgat gattatgatt ctttcatcac gcataaacgg cgaaaagcag
    15841 atttagaaag agcaaaaaac ataggcattg agcaatataa tcttgaacag caggaaaaaa
    15901 cacaaattct ttcttactta ctttctaact ataatgacgg gcgtagaaag actttctttt
    15961 gtgtggcagt caatttatta gaactttcag aattacaaga agcaatgaaa caaatacaat
    16021 ccaatgacga attgacctta ctaccattta aggcacaatg cttgtatgtg gtggaggtgt
    16081 ttcagaagat tgctgaaagg agaaatataa aactgaaact tttcaagaaa aagtgatttt
    16141 caaatttgaa attgtaacaa agattattta tatgacgaat gggggaatac ctttggaaac
    16201 agtagagtta agatttcaat atactcaaag tgaatatata aaagcagaaa ggcaatattt
    16261 aatttctagt aaaactatac ataaatatga tattgcttta gtcgctgtat ttttgttgct
    16321 gtcagtcgtt tatatgctgt tctcctcttt tagcgtattt agtattttaa tatttggatt
    16381 gataattgtt gtaactgcat tgggaagcta tttatatatc ctaatgccta tactgaaatt
    16441 caaacaaact gcaaaatatc atgaggaata tacgctggta ttttctaagg aaaacattaa
    16501 atttaaaaca caaagcattg actcagaagt gaagtgggac atttactctg cactatggga
    16561 aagccatgat ttttattact taattcaagc accacgatta tatacgttaa ttcccaaaag
    16621 aatttttaaa gacctaaacg aaaaacagct atttgaagaa attgcacaat caagagttaa
    16681 aacaacaaag catatataat gaacataccc tacaatgtaa caacttcata ttcatacaca
    16741 caaagcggtt agtcttttaa gattgactgc ttttttcata cttaaattaa gattggagtg
    16801 attgatttat gcgaatgatt ttgaataaag gacaccgtat cagagccaga gacaagaacc
    16861 ttgtctacca cttttccata gggacgcttc tgtttgtatt tgtggcggtt ctcctgctac
    16921 tgaatatcaa acagctcatg cgtaccgact gggaacattt cagcttattg gacaatggct
    16981 tgacgctttc cccctacaac ttcataacca tattgatagc gactggtatt tgtgcattgg
    17041 tcgcttttct gtattaccgc ttctgttatg acagtttcaa aaagctcctg caccgtcaaa
    17101 agctggcacg aatgatactg gaaaacaagt ggtatgaagc agataccgta caagacagcg
    17161 gtttttttac tgacctgcaa agcagatcaa gggaaaaaat cgtctggttt ccaaagattt
    17221 attatcaaat ggaaaacgga ttgcttcata ttcactgtga aatcacatta ggaaaatatc
    17281 aagaccagct tttgcgatta gaggataagc tggaaagcgg tttgtactgt gagctgaccg
    17341 acaagacctt gcatgacggc tatattgagt acacgctttt atatgacatg atagcgaacc
    17401 gtatttccat tgacgaagtg aaagcagaaa acggctgtct taggctgatg aaaaatcttg
    17461 tctgggaata tgacgtactc cctcacgctc tgatagctgg tgggactggt ggcggtaaga
    17521 cctactttct gctgacgctt attgaagctc tgctacatac gaatgctgtc ctttacatct
    17581 tagacccaaa gaacgctgac cttgcagact taggtacagt tatgggaaat gtgtatcata
    17641 ccaaagaaga tatgattgat tgtgtcaatg ccttttatga gggcatggta caaagaagcg
    17701 aagaaatgaa gcaacaccca gactataaaa cgggcgaaaa ctacgcctat cttggtttac
    17761 cgccctgctt tcttatcttt gatgaatatg tggcgttttt tgaaatgctg gggacaaagg
    17821 aaagcgtaag tttacttagc cagttaaaga aaatcgttat gttgggacga caagctggat
    17881 atttccttat tgttgcctgc cagcgtccag acgcaaaata tttctcggac ggtatcagag
    17941 ataatttcaa tttccgtgtt ggattggggc gtatcagcga attgggttac ggtatgctct
    18001 ttggttctga tgtgaaaaaa cagtttttcc agaagcgtat caaggggcgt ggctattgtg
    18061 atgtgggaac aagtgtcata agtgagtttt acacgccttt agtcccgaaa ggacatgatt
    18121 ttttgcagac cataggctct cttgcattcg taaggcagga cgggacggcg acgtgcgaag
    18181 cgaaaggcga cggcacggac tagccgttgc tggtgtggcg ttagccacgc cagcagatat
    18241 aagcccctca ttatctaaca gaggggcaca attacgctgg aaactggttc aaaaaccttg
    18301 aaaagtccgc taggaataag gattttctta gtttttagct agttgtgata atcacttgaa
    18361 aatatcacaa aatcacaaca atatcacgat tgggggtatg acaactgaat gatactgatt
    18421 ttctgaacac cttgaaagca aaacgcagtg attatggcgt atcgcagaca aggcttgcaa
    18481 tcatggctgg tatcagccgt gaacatctaa gccgtatcga agctgggaag gtagcactga
    18541 ccgaggatat gaagcacaaa ctactggaag cggtggaaaa attcaatccc gacgctccca
    18601 tgttcctgct ctttgattat gtgcgtatcc gatttcccac gctggatata cagcacgtta
    18661 tcaaggacat tctaaagctg aatatagatt atatgcttca cgaagatttc gggcattaca
    18721 agtacaccga gcattactat atcggggacg tgttcgttta cacttcacaa gacaaggaaa
    18781 aaggtgtcct gctggaactg aaaggaaaag gctgtcgtca gtttgaaagc tatctgctgg
    18841 cacaggaaag aagctggtat gacttcctca tggacgcatt agttgatggc ggtgtgatga
    18901 agcgtatcga cctagctatc aacgacagaa cgggcttact ggatattcca gaactgatag
    18961 agaaatgcaa gaacgaggaa tgtatttcta aattccgtag cttcaagaac tatggttctg
    19021 gggagctggt aaagcacaac gaagccaaca aggacggcat gggacatacg ctttacattg
    19081 gttcatttac cagtgaagtg tacttctgtg tctatgaaaa gaattatgaa caatcctcaa
    19141 aattagggat accgattgag gaagtcccca ttaaaaaccg atttgagata cgtttgaaaa
    19201 atgagcacgc ctactatgcc attagagagc tgctgaccca ctacgacgca gagctgacgg
    19261 cattttctat tatcaaccag tatatccgct ttgccgataa ggaagccaac aagcgaaaaa
    19321 gcgactggaa aaccaacgcc cgctggtctt ggttcattgg cgagggacga ccgcccgtga
    19381 agctgacgac aaagccagag ccttacactc tgcaaaggac gctgaactgg ttacaaagac
    19441 aggttgcacc gaccttgaaa atgctgaaac agatagacgc tggcaatcag atggactatc
    19501 tcaagaccat tgaacagcag gcaaagctga cagataagca caagcagata atcagacagc
    19561 agacggcttc ggtggacgaa ctgatagaaa aagaaaagga gtgattgatg atgtgggaat
    19621 tgattaagga cttactgctg gtatcacttg gcatgggtat cggcgtggtt ctgatgtgta
    19681 ttatgagtgt cggaaaggaa gctgaccgac acatgattga attaaaagaa agcgaggaaa
    19741 aataaatgaa tttcgggcaa aatttatata catggttttt aagcaacgcc cagagcttag
    19801 tgcttatggc gattgtggtt attggtatct acttagggtt taaacgtgag ttttcaaaac
    19861 ttatcggctt cttggtggtt gccttagttg ctgttggatt ggtattcaat gcaggcggtg
    19921 tcaaagacgt actcttagaa ctattcaata agattatcgg cgcatagccg tactttttca
    19981 ttatggtttg cggatacgca aacatacctt tcccactttg ggagtgggct aaggggaaag
    20041 ggggtaacgg aatggtacac agataaaaat acagcataat gaatttcatt gcttggctca
    20101 aacatagttg atataataaa gatatgaaaa taataaacga aagttttttt gataaggagt
    20161 gatattgtga aattgtgtat tgttttatct ccatataagg cttaatggaa tagacttgta
    20221 tggagtgaaa tgttatccag agagccttac attttatcaa atttcaaaat gtaaaggaga
    20281 atgggcatgg aatataaata tacaaaaatt gatttgaata aatggaatag aggtaaattg
    20341 tttcaaagct acattgataa tatgcgtatt gttatgagct taaccgtaga tattgatgta
    20401 acaaagttta ttgaattttc aaagaagcat gatttaaaat tttatccagc tatgatatgg
    20461 gctgtttcaa aggttgttaa tgaacatgat gagttcaaat atagttggga taataatggg
    20521 aatttaatta agtgggatta tatttctccg tcctatactg aatttcataa agaagatgaa
    20581 aatttcacaa agatagttac agatttttct gatagcatat ttgaatttca taaacgtttt
    20641 atattagata gggagaagta taaaacagaa cgtgcatttg tggaaaatca atctttgaac
    20701 ttttttgatg tatcttgttt accgtgggta aaatataatc attttgatgt tcatgttttt
    20761 gacgagggaa aatttcttgc accagttatt acttggggca aatatgaatt agagcaagaa
    20821 agatatataa tgcctcttac aatgaatata catcatgccg tagcagatgg attccattta
    20881 tccagatttt ttaatgaagt acaagaattg ataaactcaa tttaacagct atatacaaag
    20941 cagttagtct atacgattga ctgctttttt tcatacctaa atttaagatt ggagtgattg
    21001 cttgaaagat atattttcgg atatgcaggc aagtatcggc tgtacctaca tttcagattt
    21061 accacatcat aaacgtatgg tgtggcagga aatgaagcga ctgaaccttg ccgattatcc
    21121 gcagaaacaa ttagaagatt ttgcaggcta tgtctttggt gtcccttaca ctagcatagc
    21181 tgaagcatga aagaaaggac gtgaaaacct atgaatgaaa tgcgtgtctg tatcgaaagc
    21241 cgactacaca cttctgaaga aatccgttcc gcatggttca cacttcccat tgacgaggaa
    21301 gaactggaag aacagatagg cgttcctatc gacagtgaag attaccatat cgttgaaaag
    21361 gaactgccct ttgctgatga agtcggcgaa gatgcgacca ttgaaaaact gaatgacctt
    21421 taccatacct ttgaaagtct gcctgccgat ttacaagagg actacgggga gctgatgtgt
    21481 tactttacca gccttgacga actgcaccag caccgccatg atgtgataca ttattcatgg
    21541 tgtaaaaaca tggctgatgt ggcaaggcat ttgttggaca atgaccctgc tttttctgcc
    21601 atcgaggaac gcctgacccg ttactttgat tatgaagcct acggacagta tcttgatgat
    21661 aacggagaat ttatagaaac agaccacggt atctatgaac tgccgtagaa gtgaggtgtt
    21721 tctatggtag aggatatgcg tgtatatatc gctaatcttg ggaaatacaa tgaggggtat
    21781 ctggtcggcg actggttctc ttttccgatt gacgaagaag atgttgccga gcgtatcggc
    21841 ttgaatgaac ggtacgaaga atacgccgta catgatacgg aaaactttcc cattgagatt
    21901 ggcgaatata tctcgattga agagctcaat gaaatgtatg aaatgatatg tgagcttccc
    21961 gactacatca cggacagttt ggacgaattt gtatcaaatt atggaagttt ggaggaactg
    22021 tacgaccata gggacgatat gattttatac gctggctgtg aggatatgac cgacgttgcc
    22081 tattacttca ttgatgaatt gcaggcactt ggcgatattc cccctagttt gcagaactat
    22141 attgactatg aagcatacgg cagggattta tcctttgagg gaacttttat tgaaacaaga
    22201 tacggtatgt gtgagatacc gtggtaagaa gctggaagat cagcgacaag cattgttgct
    22261 tctgacagct ttttctattg gggacagtct gaaaaacggc tgtccttttc tattatacga
    22321 aaggagttga aacaaaaacg tgaaaaagat taaaagctac acgggtatct ggaacgtgga
    22381 aaaagtcttg tatgcaatca atgactttaa cctgcccttt cctgttacct ttacacagat
    22441 tacatggttt gtgataaccg agtttatcat cattctgttt ggggacttac cgcccttatc
    22501 catgattgag ggggcatttt taaaatactt cggtattccc gtagctctta catggttcat
    22561 gtcgcaaaag acctttgacg gcaagaagcc gtacagcttt ttgaaatcac agataacctt
    22621 tgccctacga ccaaagatta cttatgcagg aaaagccgtg aaactggata agcaggtact
    22681 acatgaagct attacggcag taaggagtgt gagctatgtt cccgattaaa tatatcgaca
    22741 acaaccttgt ctggaacaag gacaatgagg tgttcgccta ctatgagctg ataccttaca
    22801 attacagttt tctatccgct gaacagaaat tcattgtgca tgacagcttc cgacagctaa
    22861 tcgcacaatc ccgtgagggc aagatacacg ctttgcagat tgccacagaa agctcaagcc
    22921 gaagcataca agaacagtca aagaagctgg ttacgggacg actaaaagac gttgcctatc
    22981 agaagataga cgaacagacc gaagcgttag tatctatgat tggggacaat caagtggact
    23041 accgcttttt tcttggcttc aagctcatgg taacagacca gagcatgaac ctaaagagcg
    23101 tgaagcaatc ggcgtttctc acattcaagg agtttatcca tgaagtgaac cacacgctga
    23161 tgaatgactt tgtttccatg ccaaatgatg aaatcaaccg ctacatgaaa atggaaaagc
    23221 tgttggaaaa taaaataagc cgacgcttca aagtaagacg cttagataaa aatgacttcg
    23281 gctatctgat tgaacacatc tacggcaggg acggtgtcgc ctatgaagat tatgagtata
    23341 gcttaccgaa gaagaaactg aaaaaggaaa cgctgataac gcattatgac ttgatacgtc
    23401 cgacccgttg ccttgtggaa gaaagccagc gacatttacg cttggaacat gaggacagcg
    23461 aaagctacgt ttcctacttt actgtcaatg cgattgtcgg cgagcttgat tttccgtcca
    23521 gtgaaatctt ctatttccag caacagcagt ttacttttcc cgttgatacc agcatgaatg
    23581 tagaaatcgt ggaaaaccga aaagcgttat cgacggttcg caacaagaaa aaagagctga
    23641 aagatttaga caatcatgca taccagtctg gcagtgaaac cagctcaaat gtggtagacg
    23701 cattagacag tgtggacgag ctggaaacgg atttagacca gtccagagaa agtatgtata
    23761 agctctctta tgttatccgt gtatctgctc cagaccttga cgagttgaaa agacgctgtg
    23821 atgaagtcaa agacttctac gacgatttga acgtcaagct ggtacgccct gctggcgata
    23881 tgctggggct tcactctgaa tttcttcctg ccagcaagcg atacatcaac gattatgtgc
    23941 agtatgtcaa aagtgatttt ctagctgggc tgggctttgg tgcgacacag cagttagggg
    24001 aaaacacggg tatctatatc ggctattctg tcgatacggg acgcaatgtt tatctacaac
    24061 cgtcccttgc cagtcaaggc gtaaaagata cggttactaa tgctcttgct tctgcctttg
    24121 tagaaagtct tggcggtgga aaatcattct gtaacaacct gcttgtctac tactctgtgc
    24181 tgtttggcgg tcaagcggta atcttagacc caaaatccga gcgtggcaac tggaaagaaa
    24241 cgctcccaga aatcgcccat gaaatcaata tcgtgaacct taccagcgac agagaaaatg
    24301 cagggcttct tgacccgttc gtgattatgc agaatgtgaa agacgctgaa agtctggcaa
    24361 tcgacatact cacattcctt acgggcattt cttcaaggga cggcgagaag ttccccgtac
    24421 ttagaaaggc agtgcgtgcc gttacccaga gcgaccagcg aggattgctc catgtgattg
    24481 aggaattaag aaaagaagat acacctatct cttgcaatat tgccgaccat atcgacagct
    24541 tcacggacta cgactttgca catctgctgt tctcggacgg tacggtgcaa aatgcaatca
    24601 gcttagacaa ccagctcaat atcattcaag tagccgactt agtgctaccc gataaggaca
    24661 cgacctttga attactgtcc gtttccatgc tgattgtgat tagtaccttt gcgttagatt
    24721 ttatccactc ggatagaagt gttttcaaga ttgtagacct tgacgaagca tgggcgttct
    24781 taaatgtggc acaaggcgaa acgctctcta acaagctggt tcgtgctgga cgtgccatga
    24841 acgcaggcgt ttattttgtt acacagtcct ctggcgacgt gtcaaaggaa agtctgaaaa
    24901 acaatatcgg tttgaaattt gcctttagaa gcaccgatat taacgaaata aaacagaccc
    24961 ttgaattttt cagcattgac aaagaggacg agaacaacca gaagcgattg cgtgatttgg
    25021 aaaacgggca atgcttgctt caagatttat acggacgtgt gggcgtggtg cagatacacc
    25081 cagtctttga agaattactg cacgcctttg ataccagacc gccgatacag cgaaatgagg
    25141 tggagtgatg aaagaaagga taaaaagtgt gttcacaaga aaaaagatat tccgctttct
    25201 caaaatggct ctgtttgtcg tggcactttc ccttatcctg ctttcacttt tgggaacggt
    25261 ggcacacgca acgggacttg tggacgacac tatcaacacc gagaaccttt attccaaata
    25321 tccgctgtcg aattatcagc ttgatttcta tgcagataat aactgggcgt ggttgccgtg
    25381 gaactggctg gacgggattg gaaaaagtgt gcaatatggg ctttactgta ttaccaactt
    25441 tgtctggacg ataagactat atctaagcaa tgcaacgggc tatgtagtgc aggaagccta
    25501 caagctggac tttatcaacg atatggcaga cagtatcgga agaagcatac agacccttgc
    25561 aggcgtaact caaaatggtt tttctacttc gggcttttat gtgggatttc ttctgctgat
    25621 tatcttagtc gtaggtatct atgttgccta tacgggactg ataaaacggg aaaccagtaa
    25681 ggcactacac gccgttatca actttgttgt ggtgttcgta ttatcggctt cttttatcgc
    25741 ctatgctcct gactacatca agaaagtgaa tgacttttca tcagacatca gtaccgcttc
    25801 actggacttg ggaacaaaaa tcatgcttcc cgacagcgac agcaagggca aggacagcgt
    25861 ggacttgata agggatagcc tattttccat acaagtacaa cagccgtggc tactgcttca
    25921 atttggtaat tcgggtatag aggaaatcgg ggcagaccgt gtcgaagctc tcgtatcggc
    25981 aagcccagag gacgaggacg gcaagaccag agaggaagtc gtgaaaacag aaatcgagga
    26041 caacgacaac aacaatctga cgatactgca ggtggtaaac cgtttaggta tggtgttctt
    26101 cctcttgttc ttcaacttgg gtatcacgat atttgtattc ttgcttacgg gtatgatgtt
    26161 gttcagccag atacttttta tcattttcgc tatgtttctg cctatcagct ttctattaag
    26221 tatgataccg agctatgaga gcatggcaaa acaagccata gtccgtgtgt ttaatacgat
    26281 tatgacaagg gcaggtatta cactgattgt cacggtagcc tttagtattt ccagtatgtt
    26341 ctacaacata tcgacggact acccgttctt tatggtggcg ttcttgcaga tagtgtattt
    26401 tgcaggtatc tacatgaagc tgggcgattt aatgagcatg ttctctttaa acgctggcga
    26461 cagtcaaagc atgggacggc gaatattccg cagaccgtat ctgttcatgc gacatagggc
    26521 tagacgtatg gaacataggc ttgcaagagc tgttagtgct ggtggtgtcg ctggtgcggt
    26581 ggctggtggt gtcgttgcca gttcaaaatc tacacgccag aatggaacaa agaacaatcg
    26641 tggaaataca agctccagca tggggcagaa agcaggttca gccgtgggag ctgtccttga
    26701 tacaaagaat aaggtcaagg acaaggcaac tgctgtcaaa gaaaatatca aagatatgcc
    26761 gacacagact gcctatgcgg ttcactctgc taaggaaaag gcgaagtcca gtgtgtctga
    26821 tttcaagcgt ggcgtggtgc aggaaaagga aaaccgccag tctggacgct taaagaagca
    26881 acagctacat agacagaata tcgctgacaa gcgtatggaa cttcaaaagg cacaagaatc
    26941 aaggcaggca ggtaagaaaa cggacggatc agcgacaagc ggagctaccc gtccccatga
    27001 aagaccagct accacttcta aggttaatgt agaaaaacag caggaagtca aacgccctgc
    27061 catagcttca accgtgaaga atgatgaacc tatcaagaaa aatgttgtca aggaacgtcc
    27121 actatcttct ggcgatacat cagcaaagac cgtcaatcaa gaaaccacaa gacagaatta
    27181 caagactgac cgtgttacga aaacaaccgt tcagaagcat acacagcaga ataccgagaa
    27241 gaacaaacga accgtattca agaaaggaca aaagaagaaa tgaaactaag acatatcgct
    27301 attgttggca gtctgttttc tatcctcttt tctctcgtat tattctttgg ggttctgatt
    27361 agtgccgagg acgacgacgg gggcaatggt tattcctcta attatgtagg tatgaaccta
    27421 tctgccgaag tcttgaaaca tcagcctatg gtagaaaagt atgccaaaga aaacggtatt
    27481 tccgagtatg tcaatgtgtt gctggtaatc atacaagtgg aaagtggcgg tacagccgag
    27541 gacgttatgc aaagctctga aagtctggga ctaccaccca actcactaga tacggaaagc
    27601 tcaatcaagc aggggtgtaa atattttgcg tccctgcttt cttcctgcaa aaatcaaggt
    27661 atcagtgata taaatgttgt gatacagtcc tataactatg gcggtggcta tgttggctat
    27721 gtggcaggaa acggaaaaaa gcatatgttc aaccttgcgg aaaacttcgc aagggataag
    27781 tcgggcggtc aaaaagtaac ctatacgaac cctatcgccg tggcaaaaaa tggtggctgg
    27841 cgttatggct atggaaatat gttctatgtg gagcttgtga accagtacct tgttgtcaat
    27901 caagtatcgg gcgagctggc acaaaaggtt atgaatgaag ccttaaaata tcaaggttgg
    27961 aagtatgtat atggtggcag taacccgaac acttcttttg attgttcggg actggtacag
    28021 tggtgctaca acaaagctgg cgtatcttta ccgagaaccg cacaaaatca gtatgacgca
    28081 acccagcata tcccactatc gcaggcaaag gcaggcgatt tggtcttttt ccacagtacc
    28141 tacaatgcag gaagctatgt aacccacgtc ggtatctatg taggaaacac tcagatgtat
    28201 catgcaggcg accctatcgg atatgccgat ttaaacaatg cttattggca acagtacttg
    28261 atttgtgctg gaagaataaa acagtagaaa ggattggaaa ccatgtttaa aaagaaagaa
    28321 aaaccagtaa aagaaacgaa agtccgcact atgaaagtag gcacacataa gaaatctgtg
    28381 attgctctgt gggtggtgct tatcgccagc gtatcatttg gcgtgtataa gaactttacc
    28441 gccattgata tgcacacggt acatgaaacg gaaaccattc agttaagact aaatgacacc
    28501 aacggagttg aaaattttgt gaagaacttc tgtaagtcct actacacatg ggacaacagc
    28561 aaggaagcta tcgaagcaag gacgcaggca atcagcaact atctgacaaa ggagttgcag
    28621 gacttgaacc ttgataccat taggacggat ataccgacaa gctccactgt tacaaatgta
    28681 ctgatttggg atataaaaca gtctggaacg aacgatttta tcgctaccta cgaagtagat
    28741 cagcagataa aagagggaga acagacaagc aatgtcaaag caacctatac cgtcaaagtc
    28801 catgtggata aagacggcga tatggttatc gtacagaacc caaccattgc accagcgatt
    28861 gaaaaatcag actatgagcc aaaggcacaa gaagccgacg caagcgtgga cgcagatacc
    28921 gtcaatgacg ctaccgcatt tttggaaact ttctttaagc tgtatccgac agcaacagaa
    28981 aaagaacttg cctactatgt ggctggcaat gtgcttgaac ctatcggcag ggactacctc
    29041 tattccgaat tagtcaatcc tatgtttacg gatgacaggg ataatgtgaa agtcagggtt
    29101 gcggtgaaat tcattgataa tcagacaaag gcaatgcagg tatcacagtt tgagcttgtg
    29161 ttacataagg atagcaactg gaaaattatt atgtaagata ttttgttggc aggctctatt
    29221 ttcggatata gtctgccttt tctatagcaa atgtgctgca ctaacaagcg atttcattgt
    29281 attttaagca attaaatctt tcttgacaat tttatcttaa atcatatatt tatggatatt
    29341 gactttctgc ttgcaataag ttataatatg actataaaac taaattagtc atataggagg
    29401 cgtgtggtat gtataaagaa gttggtattt cttcaaataa tgttccgata acactttcta
    29461 tttggtatac agaaagaaat agtcctacca ttgttttttt gccgggtact atgtcacacc
    29521 ctctcatgta tgagaatttt ctatgtggtt tgagtgaaag aggatttaat ataataggca
    29581 ttcactatct ttcacacgga aaaagcccta aaatcaaaaa aaattataca atggaagatt
    29641 tgctgcataa tgtatatgac gcaacgactt acggaataga aaattttggt gaaaacatag
    29701 ctgtcatggg ttccagtcaa ggtgggattt tagctgcaat ggcggcgggt agggacacaa
    29761 ggttaaaagc cgtttttcca cataatatca tgctgactac attaaaagaa actatgagct
    29821 taacaaaata ccccgcatgg tcacaccgtt ttatggggct gatacagttg ggctttcgtg
    29881 taggtggaaa attgctgcct aactataagg ttccaggaga cgcttattta gactatgaca
    29941 gagtatttta tagtgaacag gcaaaacaag attgcttaaa tgaccctatg cttttaccct
    30001 attatccttt aagatttgtc gccagcttat ttaatgctga cttatcgtgc ttggagaatg
    30061 gggctataca atgtcctgtt attttaatta ctgccaaagg cgatccgctt tttagatttg
    30121 attataccaa caaagtattt gatttaattc atgtaccgaa aaaggaatta cttactttag
    30181 agcttaatcg tcacatgatt tttaatgagg acacggacac tacaataaac gcattggaag
    30241 ctaccttgca taaatatctt ggatagtata ttgattatat aatcagtaaa tgttatactt
    30301 ttcatgacga taaaaacatt ttagtcataa gggggttatg agtttgccac gatttacgga
    30361 acaagaaaaa gaaatcataa acagtaaact tttgatagag ggagaaaagc tgttttcatt
    30421 atacggctta aaaaaagtaa cagttgatga tttagcagct gctgtaaaca tatcaaaagg
    30481 ttcattctat gctttttatc ccagtaaaga acatctatat gtggaaatca attttcgctt
    30541 acaaaaagag ttatttacaa gtatagaaac aactgttaaa aagaaaaagt ataagaacca
    30601 tagagattta gcaaaggacg ttattatgct aagtttaacg ggggtgatta cttcgcctat
    30661 tctttcacag attgatttat ctttaatgga ctatctgcaa agaaaaatat cgtcagatat
    30721 ttttgaaaat catatgtaca gtgatattcg tatattggag atactggaag gtttaggggt
    30781 tgtatttttg gttccgcata cggttatcat aaaatccctg tattctgttt tatcctgttt
    30841 ggaacaattc aaagaggacg aagaattgaa tatggtacaa aatcttttag tgaatggtat
    30901 tattcagcaa gtagtagcag agtaataatt ttgtttggtt atttgacagg caagtggcca
    30961 aacaaatttt ttacagttta tatgactata tatctataaa cgtcataaag ttaaaggagg
    31021 aaagaataat gattgatgta aaaaaactgt attttagtta tactgacaaa ccgtttgttg
    31081 agaatgtaag tttccatgtt gggagaggag aaattttcgg ttttttagga ccctctggtg
    31141 ctggaaaatc aactattcaa aaaattttaa ctgggcttaa tacaagttat aaaggcagcg
    31201 taaaagttgc aggtacagaa ataagggagc atacaaaccg gttttatgaa aatattggag
    31261 tagactttga attttcaacc tgctacgaga aattcacagc acgacaaaat ctcgcctatt
    31321 ttgcttctct atatgacaag caacctcggt ctattgatga attattgcat atggtaggct
    31381 tagaaaatga tggggacaaa aaagttgctg acttttccaa aggtatgcgt tctcgcttga
    31441 atttcattaa agcgttagta catgaccctg atatattatt ccttgatgaa cctacaagcg
    31501 gtcttgaccc tacaaataat cgtttaatga aagatattat tcttgcggaa aagaaacgcg
    31561 gaaaaacgat tatcattact actcacaata tgtatgacgc aacagagcta tgtgaccaag
    31621 tagcctttat tgttgccgga aaagtcagtg cattggatag tccacataat ttgattatgt
    31681 caaggggagc tgccaaaata cactatacct actatgataa aggcgagaaa accggagaat
    31741 gtctgcttga tagaaccaca gaagataagt tgttaaaaag tttaatttcc gaaaaccgtc
    31801 tgctttctat tcatagtagt gagcctacat taaatgatat ttttgttgat attacaggga
    31861 ggacacttca atgagattgg gacgtttaat ttgtggtgat atacatttcc aatggaaata
    31921 tggtttctat tttatctact ttatattaac agttttatat gtgtgtggga ttgccgcttt
    31981 gacgggacat tggaaaacgg atattgcttc tatcatgata tactctgacc cggcagctat
    32041 gggattgttt tttatggggg cgattgtcct tttagaaaaa agccaaaagg tattaaatgc
    32101 tatggtagta tcgcctgtaa aagtatcgga gtatatactt tcaaaaacca tcgcattgat
    32161 tgctatttct acaatcattg cggtgatatt gggatttgtt tccggcagca atcatttact
    32221 tggtatcgca gttggtacag ctttgacatc agcaatcttt actatgcttg gcattatcgc
    32281 ggctacaaaa atttcaaact tgaaccaatt tctcattgtg attatgccga tagaaattgt
    32341 ttgctttgtt ccacctatcg ttggtctgtt tgtaaagtta ccagatatat tccgcttttt
    32401 tccgttcaca gcttgcatga acctcataac tggaaaaagc attttactat cgtttgatat
    32461 ggtacttgta attgctacgt tgattatcct ctacatagtt gcgcgacata cagttaagca
    32521 tatgtggaga agtttgggag gtgtaaagct atgaaaagga tattatccag taccatacag
    32581 gtatttaagc aaatcaagag cgaccctatg atgtttgcag cttgttttac gccttttgtc
    32641 atgggagctt tgattaaatt cggtattccc tttcttgatg gaataacaga tttttctttg
    32701 caaacatact atccgatctt tgatttatta ctttctatca tggctcctgt attgctctgc
    32761 tttgcatttg caatgattac attagaggaa atcgacgata aagtgtcacg gtacttttcg
    32821 attacccctc ttggtaagtc ggggtatctc tttacaaggt tgggagtacc ctcaattatt
    32881 tcggcggtca tcgcttttat tgtactgttg cttttttcat tagaaaagtt gtccattgga
    32941 atgacaatat gtttggcgct tctcggctca gtacaggcaa ttattgtttc gcttatgatt
    33001 ataacattat caagcaataa attagagggt atggcagtta caaaactagc tgcacttaca
    33061 ttactcggaa taccagctcc cttttttatt gatagttact accagttcgc ggttggcttc
    33121 cttccatcat tttgggtagc gaaagccatg cagaatgaag cggttcttta tttctccata
    33181 ggattgatgg tagctttggt ctggtactac ttccttataa aacgtctgtt taggaagctg
    33241 gcaggataag aaggcatatt atggaaaaag gtagacttct gcaaaacgaa aatataaata
    33301 aggctattca ggtttccaaa atcacactaa tcatcaacct gggtttatcg ctacttaaat
    33361 ttgctgccgg atatatagga aagtcaagtg ccatgctgtc ggacgctgta cactcggctt
    33421 ctgatgtatt aagcacaatc gttgtgatgg ttggaattaa aatatcagaa aaacagcctg
    33481 ataaagaaca tccttatggg catgaacgta tggaatgtgt ggcttccatt attctctctg
    33541 ttgcattggc tataacgggt gctggaatag gatattcagg tatcaagaaa atcttttcag
    33601 gacagtacaa tacgctttct gtctctagtg gaatcgcact tacggcggca gtcttatcca
    33661 ttgttataaa agagtggatg tactggtata caagaagtgc tgcaaagcat acaaattctg
    33721 acgcacttat ggctgacgca tggcatcacc gctctgatgc actctcgtcg gttggttcct
    33781 taattggcat tttaggagca cgtttaggct atgctattct cgaccctatt gcaagcattg
    33841 tgatttgcgg ttgtatctta aaagctgctt tggacatttt caaagaaagc attaacaaaa
    33901 tggtagacca tagttgtgac aatgctactg aaacgaagat tagggaagtt gtgcttcagc
    33961 agcaaggggt tgatggtatt gatgaattaa aaacccgcat gtttggagcg aaaatgtatg
    34021 tagatattga aatattagct gatggtaatt tagctcttta tgacgctcat agaattgctg
    34081 agggagtcca tcaaacgatt gaaaacaact ttccacaatg taaacattgt atggtacatg
    34141 ttaatacgaa tgaacttccc tattctccat aggacaggga agatgattta agactattga
    34201 aagggaaagg aggggaagta ggttatggaa caatactggt ggattatcat tgttatggtt
    34261 ttgattgtcg ttggcttact taaaattgta attatgaaga agtataacca aaggagaaat
    34321 tctaacaatc gttcaaaaaa tagcgaggat gacaagatgt attaagagca attatccgat
    34381 ggtatgagat atatacctat gaaatttgaa cgataaatcc cagtttgtaa aagtcaataa
    34441 atccaaagat aaatttgtat ttgaaaggag ttttgaagaa tgaaaaaata tgagtatgta
    34501 gaaattaatt ataatgcgaa aggagttgta ttcctttgta ctgataaaca caaagaaatt
    34561 attgacagtt atgcaaaaac ggggtatcac tacgcagggt ttgtacccac agaaattgac
    34621 gcaaaaggct gtatgcgaaa gatagattta atttttgaaa aagaagatta acttatttta
    34681 gggagttggt tctaatgata ggacttaaaa gacgagatat gaattatatt atgaaagttg
    34741 gtgctaaagc tcaaggtgtt tctgtatctg aatttagggc tatgctacaa gataccatag
    34801 acaaaactat ggaaagtaca gacccagacg tgcaggcaaa ttttaaacga tactttggaa
    34861 ataagcggcc aacaccagag gaatatattt atacgattac caaaaagaca aaggttaagg
    34921 tttaaatgtt gtaaaggggg cttgccgtat gaaacgcttt aaatacaatt cagtatattc
    34981 aagcccaatt taacagcata ctatttacaa ttaaatacgc tataccagta gctcgtacaa
    35041 aatttatatt ttgtgcgggc ttttttaata cccgaaaagt tttttaatta ttttcatttt
    35101 tttggaagca aatcacacct tgctgtacag atatggtgcg gaaagaggga taaacacttt
    35161 tcaattcata gctggaaagg aggtgagatt atgaaaccgt ctgacttcca aaaaacaatt
    35221 cagtgtcagt ttgaatgtaa gctgaaaaaa gttgtcaaag gtattatctg gaactaccgc
    35281 aaagaattaa gacgaagaaa aaacaaggaa atatctttta gcgaattacc agacattacg
    35341 gtagaaagtc tggcaacatg ggacgactac gaaacaaact acacaatttt taatgtgtgt
    35401 ggcatagata ttaaagtcct tgatgatgaa cttgcagaag ctctcaaaca gctacccgaa
    35461 aaaaacgtaa caatgtattg atgtattact tcttggaaat gagtgataca gagattgcag
    35521 atattatgaa ttataccaga gaggctgtgt tcaaacacag acaccgtgca ctagaaacta
    35581 tgaaagagat actaaatgaa aagacaagac agtaaaggac gcaatttgcg tcttggagag
    35641 agccagagaa aagacggaag atacgtttat aaatataccg atatattcgg gaagccacag
    35701 tttgtatatt cttggaagct tgtacccaca gacaagacac ctgctggaaa acgtgatgat
    35761 atatcgttga gggaaaaaga aaagcagata aaaaaagacc ttgacgacgg tatcgacacg
    35821 gtgggcggta aaatgacagt ctgccagctt tacgaaaaaa agaacaatca aagaaagaac
    35881 atcaagagaa acacggaatt aggacgacag tatcttgtga acgcactgga aaatgaccca
    35941 ttgggtatga gggcgattga tacggtcaag cagtccgacg ctaaagaatg ggctatcaga
    36001 atgaatggca aaggttattc ttacaagacg atagataact ataagcgttc cctaaaggct
    36061 tcattctata tggcgataca agacgactgt atcagaaaaa atccttttaa ttccaagttg
    36121 agtgatgttc tggaagatga tacggaagca aaggttatcc tgacaccaga gcaggaagaa
    36181 aaactccttg cctttatgga aacagataat gtttacagca agtatcgtga tgaagttatt
    36241 cttctgctgg aaacgggact tcgtatctct gaattatgcg gactgacaac acacattgat
    36301 atgcttaacc gtgtgattaa catagaccac cagctattgc gtgatacaga ggtgggctac
    36361 tatatttcca aagctaagac aaaaaacggt aagcgagagc ttccattaac agaaagagca
    36421 tatcaagcac tcaagcgaat tttaaagaac cgaggaaaag cacaaccgct tgttgttggt
    36481 ggttatagca atttcttatt cttgaaccgt gagggattgc caaaggtagc aggaaattat
    36541 gaaagtatgg tgcgtgggtt gattaagaag tacaacaaga cccataagga caagttacca
    36601 aacatcacgc cccattcatt cagacatact tactgtacga acatggcaaa taagggaatg
    36661 aacccaaaca ccctgcaata catcatggga cacgcaaaca taaccatgac attagggtat
    36721 tatgctcacg gtactttcca gtctgcaaag gcagagttag aaaggctggc ttcttaatat
    36781 cgaaaatgta tcttactact tatttactac ttttggttgc attttcatgc tggtaaatgc
    36841 cagcttatgc aaggtatctt ccaaaaagaa aatgcccata cagcccgaaa atgcgggata
    36901 tatcggcatc taccaactta tgaaaagata atcagaaatt tagtaagttt ttataataaa
    36961 aaaataagag gctgtccaaa aatagagata agcctttttt cataatcgga attataaaaa
    37021 tacacttgat ttcatatgaa aaagagtgtc tcataaacta aaaagtttac tttgagacac
    37081 tacctttttt attcttattt atactttgta gacatattat aatatgcgtt tcactggtat
    37141 ctgaacttct gtcaaccagt cttcttcact ctctttattc catataccat ctatataaga
    37201 ttctctagga tttccaacca ctttatattt attgtcctct atccatttaa atgcatctgc
    37261 ataagttttt cctaatgttg aatatggacc attgtgtaat atacaaactg cattttcaac
    37321 tgcatctaat tctttaaatt ttaatatatc cgtatcttcc tttaaatcat ttatagcttg
    37381 acacatttca acatccatat cagtctctct atattcacca tcatgatata tattaaaaca
    37441 ataatcagga actgcacatg tacaccctgt tttcatcatt tcttcagcca ttacatttgg
    37501 aaatagatta aaaagttcat catatccctt taacactttt ctcatagaag caactttaac
    37561 ttttggtaaa gacttaataa ttacatcatt catattgact tctcctttca aattcataag
    37621 gtaattacgt agtttaatta ttttatattc ttcactttta attacatcca acatttcttt
    37681 ttctttattc attaaaatat tttcaacttc ttcaggattt tctacagctt tttttatctc
    37741 agctaatgat aaacccattt gttttaaagt tattatctga tgtaatttag gtaattgctc
    37801 tgttgtataa tacctatatt ttgtaaattc atctacatat tctggtttta acaaccctat
    37861 atcttcataa taccttaatg tctttgtagt tattttattt attttagaaa acattcctat
    37921 agaaaacatc tcatcacctg cgcattagat ttgattaatt taagtttaaa ctttcccctt
    37981 aggttaaagt caatacaaaa taaattataa ttgtattaat tcttcaattc tatctccact
    38041 caataccaaa atatcccctg tttttataat ggttgaacct ttaggtatta aaacctgttc
    38101 ctttcttttt atcataacta ttaatatatc gtctggtata ttagaatcta ttatactctt
    38161 attagcccat ttattaccct catcaacatt tacttcgata agtttagagc taatttcccc
    38221 atcataatca ttaaatgttt ttaatacagg tgtattatca tcaactaaat ccaactttct
    38281 ggctacagtt ggtatcaatg aaccttgtat tcctactgaa agcaacgcta taaaaaatat
    38341 tatatggaat atgtcattct ctatggctac tccttgagta acagcataaa ttgcaaaaac
    38401 tactgaagcc gcacctctaa gacctaccca cgatatgaat aatttttctt tatttgagaa
    38461 tttaaatttt gctagaacta taaatgttgc taatggtctt gcaataacta tcatgaatat
    38521 agatatggcc attgctatac caaatacatt tggtaactta gaagggaaag ataacaatcc
    38581 taacataaag aataacatta tttgcataat ccatgaaatt ccatcaaaga aatgaaccaa
    38641 acttttttta agaggtatcc tactatttcc tataataata cctgaaatgt aaacacttaa
    38701 atatccattt cctccaagat actcgctaaa tgcataagaa agtattgcaa tagctgttat
    38761 aaatatagta taaaatcctt ctatctcgaa gtttgaatgc cttaaaatat aaacagataa
    38821 ttttgctagt ataaatccta tagctaaccc aaaagctatt tgttttatta ccattggtgc
    38881 aataagttct accccagaat tattcatcaa tgtcaaaaga attagtgtca acatatacgc
    38941 tattgggtca ttacttccac tttcaacttc taataatgat gcaagtgaac cccttaagtt
    39001 tagtttttgt gaacgcaaca cagcaaacac agatgcagca tctgttgaag ctgcaattgc
    39061 cccaattaat aaaccttcaa gtaaactagt tcctattacc atgtggcaaa acatacctgt
    39121 aaccccagac gtaattatta cacccagtga agacattaat atagaaggta tagcaacagg
    39181 tttagccata ttccagtttg ttccaaaccc tccaaaaaac attataaata ttagtccaat
    39241 agaacataat tgttttgtta attcatagtt ctgaacctcc catgactaaa gtcacagagt
    39301 tcctgcttca tagaattttg catactaaaa tatgtcttac taattctcca caggctatcc
    39361 ccatagttcc tacggttctt acataagtta tttattttga attgttatta gtcttaatcc
    39421 ttcttttaat atatttatac ttgcatttat atctctatcg tgagtttcat tacaactagg
    39481 acaaatccat tctcttatat ttaagttctt cacttcctcg tttttatacc cacatttatt
    39541 gcatatttgt gaacttgcaa agaatttacc tacctttaca atttgtctac catgccaatt
    39601 agccttgta
//
LOCUS       DAELXX010000024        37804 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.024, whole genome shotgun sequence.
ACCESSION   DAELXX010000024 DAELXX010000000
VERSION     DAELXX010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37804
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.024"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(23..766)
                     /locus_tag="KPH29_001206"
     CDS             complement(23..766)
                     /locus_tag="KPH29_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBG1466830.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHRWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKGKLLDVDYTNI"
     gene            932..3499
                     /locus_tag="KPH29_001207"
     CDS             932..3499
                     /locus_tag="KPH29_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG1466831.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYSYNLMNTFIYNNSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAVDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVTYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            3729..4133
                     /locus_tag="KPH29_001208"
     CDS             3729..4133
                     /locus_tag="KPH29_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG1466832.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            4157..4654
                     /locus_tag="KPH29_001209"
     CDS             4157..4654
                     /locus_tag="KPH29_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG1466833.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            4657..5058
                     /locus_tag="KPH29_001210"
     CDS             4657..5058
                     /locus_tag="KPH29_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBG1466834.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            5076..5564
                     /locus_tag="KPH29_001211"
     CDS             5076..5564
                     /locus_tag="KPH29_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG1466835.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            5591..6346
                     /locus_tag="KPH29_001212"
     CDS             5591..6346
                     /locus_tag="KPH29_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG1466836.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            6346..7209
                     /locus_tag="KPH29_001213"
     CDS             6346..7209
                     /locus_tag="KPH29_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG1466837.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKKKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            7256..7735
                     /locus_tag="KPH29_001214"
     CDS             7256..7735
                     /locus_tag="KPH29_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HBG1466838.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            7800..9107
                     /locus_tag="KPH29_001215"
     CDS             7800..9107
                     /locus_tag="KPH29_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG1466839.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETDMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVRGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIAHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            9418..9558
                     /locus_tag="KPH29_001216"
     CDS             9418..9558
                     /locus_tag="KPH29_001216"
                     /inference="COORDINATES: protein motif:HMM:NF021468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HBG1466840.1"
                     /translation="MNGEGCILSNKITKECYKTGYMIREKSIDTYPDSGRGFMTWDEN
                     GE"
     gene            10070..11164
                     /locus_tag="KPH29_001217"
     CDS             10070..11164
                     /locus_tag="KPH29_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1466841.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQTQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSLLSENKEYKFLIEKLSLLEE"
     gene            11169..11369
                     /locus_tag="KPH29_001218"
     CDS             11169..11369
                     /locus_tag="KPH29_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc_N domain-containing protein"
                     /protein_id="HBG1466842.1"
                     /translation="MENLMEYLPLLIPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            11369..12286
                     /locus_tag="KPH29_001219"
     CDS             11369..12286
                     /locus_tag="KPH29_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1466843.1"
                     /translation="MNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKTT
                     TMKMVLGLLEPDSGTIDVCGEKVTYGRTSSNRHVGYLPDVPEFYNYMRPLEYLSLCGE
                     ITGLSKKEIKIRSEELLSLVGLGNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDEP
                     TSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLSE
                     IKSMHGKDRLLLEFASNNEIQKFKSSGGIKSLLKDSEETNMEIVLHGKDIKAIQKTVI
                     STLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            12283..13041
                     /locus_tag="KPH29_001220"
     CDS             12283..13041
                     /locus_tag="KPH29_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1466844.1"
                     /translation="MREYIAFTKKEFKENIRNYKLLSLTILFLVFGIMSPLSAKFMPD
                     LIAHFAPTLKVTTAPTALDSWTQFSGNISGLGTSLTLIVFCNILSNEYSKGTLVIMLT
                     KGLSRSSVVLSKFSIAVIIMTIGFWLSFLCTYGYTMYFWPTANLNHIIFSAFNLWLIS
                     IMYISILILGCVLFRPAFASVLLVLAATAILSLISIPKQIAPYTPNFILSKNIDLVSG
                     KVTPPEFIIPIIVTIVISIVCLLLAVVLFNKKAV"
     gene            13424..14497
                     /locus_tag="KPH29_001221"
     CDS             13424..14497
                     /locus_tag="KPH29_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBG1466845.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            14567..14830
                     /locus_tag="KPH29_001222"
     CDS             14567..14830
                     /locus_tag="KPH29_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466846.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            15036..16013
                     /gene="bioB"
                     /locus_tag="KPH29_001223"
     CDS             15036..16013
                     /gene="bioB"
                     /locus_tag="KPH29_001223"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBG1466847.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSHYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMVFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEL"
     gene            16241..17254
                     /locus_tag="KPH29_001224"
     CDS             16241..17254
                     /locus_tag="KPH29_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG1466848.1"
                     /translation="MSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKIMKE
                     KNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNTDED
                     AKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETLKGI
                     VKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYNSAY
                     VFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDVSVVGFD
                     DIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRDSTK
                     ESE"
     gene            17296..18186
                     /gene="rbsK"
                     /locus_tag="KPH29_001225"
     CDS             17296..18186
                     /gene="rbsK"
                     /locus_tag="KPH29_001225"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBG1466849.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIKVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKKLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            18234..19196
                     /locus_tag="KPH29_001226"
     CDS             18234..19196
                     /locus_tag="KPH29_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBG1466850.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            19250..20761
                     /locus_tag="KPH29_001227"
     CDS             19250..20761
                     /locus_tag="KPH29_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBG1466851.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            20745..21743
                     /locus_tag="KPH29_001228"
     CDS             20745..21743
                     /locus_tag="KPH29_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBG1466852.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            21927..23189
                     /locus_tag="KPH29_001229"
     CDS             21927..23189
                     /locus_tag="KPH29_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG1466853.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIGGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFQMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARLMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            23209..23952
                     /locus_tag="KPH29_001230"
     CDS             23209..23952
                     /locus_tag="KPH29_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HBG1466854.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSEHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            24125..25510
                     /locus_tag="KPH29_001231"
     CDS             24125..25510
                     /locus_tag="KPH29_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG1466855.1"
                     /translation="MDNLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KECLKKHGFSIEGLEGMDTAFVASYGEGRPVIAVLGEYDALDGLSQKVSTTKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDNCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTIMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKD
                     TVGSFEYTPII"
     gene            complement(25847..26491)
                     /locus_tag="KPH29_001232"
     CDS             complement(25847..26491)
                     /locus_tag="KPH29_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG1466856.1"
                     /translation="MNNLDKNNVNEVLDMLLRTRRSIRSFTKEVPSKGLIEEIIETGR
                     LAPYAILSVAKQTDFRHFFVISNNSLQMNKIKTILVDVIKTQISVNEKKAKNNITLQS
                     FITSLKMQVEKGVLGIGTAPYLIIVAERRGIPPIEQKSLSHVMQNMWLKSTALGLGFE
                     LVSVIGRLSDNKDFCDILGVPTGEYGFDACAIGFPTTSVTREGRAIPHPSIKWI"
     gene            26755..27369
                     /locus_tag="KPH29_001233"
     CDS             26755..27369
                     /locus_tag="KPH29_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HBG1466857.1"
                     /translation="MKKVSIDRRTIRTKKMIRSALAELIDEKGFNNISVTDLTQRADI
                     NRGTFYLHYVDKYDLLERIENEIIQEIEEETKHLDSSNMMEIDTSSEPLPFMVKLFEY
                     FRKNSVIIKSILGPKGDPTFERKIKKFIETNLFEKQKLINFNIDEAIISEEYFIQYIL
                     SADLGVIQYWLEKNMKESPEEMALILARMSLLGPLRAVGIRKDS"
     gene            27590..27730
                     /locus_tag="KPH29_001234"
     CDS             27590..27730
                     /locus_tag="KPH29_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466858.1"
                     /translation="MKNENISYLALGLCFGVVLGSLFKNLAIGLSFGLVIGVVLDSKK
                     NK"
     gene            complement(27960..28407)
                     /locus_tag="KPH29_001235"
                     /pseudo
     CDS             complement(27960..28407)
                     /locus_tag="KPH29_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002599264.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            29177..30016
                     /locus_tag="KPH29_001236"
     CDS             29177..30016
                     /locus_tag="KPH29_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466859.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            30006..30449
                     /locus_tag="KPH29_001237"
     CDS             30006..30449
                     /locus_tag="KPH29_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466860.1"
                     /translation="MYSNTYNNENFMRARAFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            30460..30951
                     /locus_tag="KPH29_001238"
     CDS             30460..30951
                     /locus_tag="KPH29_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466861.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
     gene            complement(31153..31881)
                     /locus_tag="KPH29_001239"
     CDS             complement(31153..31881)
                     /locus_tag="KPH29_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBG1466862.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
     gene            32006..33094
                     /locus_tag="KPH29_001240"
     CDS             32006..33094
                     /locus_tag="KPH29_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466863.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLIIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKELMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            complement(33532..34380)
                     /locus_tag="KPH29_001241"
     CDS             complement(33532..34380)
                     /locus_tag="KPH29_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466864.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMPNNMNSNMGMPNNMSNNMNSNMGMPNNMPNNMNSNM
                     GMPNNMPNNMNSNMGMPNNMPNNMNSNMGMPNNVPNNMNSNMGYNMNNTMGIPNFSCN
                     QNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSIQGIPQATNIEE
                     FDEEEM"
     gene            34579..34944
                     /locus_tag="KPH29_001242"
     CDS             34579..34944
                     /locus_tag="KPH29_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1466865.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            34967..37354
                     /gene="cadA"
                     /locus_tag="KPH29_001243"
     CDS             34967..37354
                     /gene="cadA"
                     /locus_tag="KPH29_001243"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBG1466866.1"
                     /translation="MEANNLIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLIVNIKESFNEDTTIEKIIDIIDSTEPGLDIQISSKENASSKTSIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTVNISNNFKEDDVINKIKEIINSTEPGLD
                     IQVGSTDKVKGRTTEKSGAVNDTNKKELVPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALTIGESSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKFNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKVYRGYIVISDEVKEDSK
                     NAIRSLKAIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
BASE COUNT        14712 a         3938 c         6813 g        12341 t
ORIGIN      
        1 taaagtaagc atgtattatt acttagatat ttgtataatc aacatctagt aatttacctt
       61 tttggtgaaa atgattaata agttttttag gataattcat tattaattca tctggaaaat
      121 taatttcctt taagatattc tcagaatact catagttacc tatgtcaaaa caaatgtgag
      181 catctgaagt tagtatgatt ttagcaccat attttttgca aagtgtagca atatacttac
      241 agttatccga gctaccattt cttgacacgc ctttaataga acaattattt atttctatta
      301 agatattctt ttctatagct aatttaatga ttaattcata gtcgattggg tagtttgggt
      361 ttccaaggtg tcctaatatt tcaatattat catgcttatt tattgcattt attaaagctt
      421 tagtattatt ctctaatgta tttggagaaa aaacagcttc gtgaaatgac aagataagat
      481 aatctaaccg aggaattaca aaaggctcta ggtctatgtt tcctttaata tctagtatat
      541 ttgcttcaca gcctcttaaa attataatgt tatttatgat tcttgggata ttttgaatgt
      601 tgtgaaagta ccatctgtgg ggagaaccag gcatcttagg accatgttca gatgtaccta
      661 gtatcttaat tccattttct ttacaataat taatattttc tagtaaagtg ctatagccat
      721 gaccactgac tatagagtgg gtgtgtaaat ctgataaaaa attcatataa attcctcctc
      781 tataaaagat gagggtaacg taatttatat tataaaacga ataggaaaga aaatcaatag
      841 ttacataatt agacaaaaat attaaaatat tgtagaatta agcttaataa taaatgttta
      901 acagtattac tgcttatagg ggaggttaac tatgacacaa aagaagctat caaggaaaga
      961 aaaagtttta atttgcttga aaaggttaac agaagaacac ttgaacttta ataatggcat
     1021 tagatttgga tttgatgcag aatatatagc tactgaagta ggtataaata gaagtaatgc
     1081 tagtaaggaa ttaaatcaat taataaaaga aagtattatt ataaagataa agggtaagcc
     1141 tgttcagtac ttatataaag aaagtattga gagtattttg ggaaagaaac taaataaagg
     1201 gatatttgat aatattaact ctataaacga atatagtgaa aatgattcta tcaaagatga
     1261 gtatatttta gagagtataa aaaaagagaa caatattttt aatttagtag gatgtgatgg
     1321 aagtttaagg tcccaaatag aacaggctaa agcagcagta atatatccac ctaaggggtt
     1381 gagtacatta ataataggac ctacaggagt tggtaaatct atatttgcag aatatatgta
     1441 taagtatagt ttaactataa aagaagatag tgataatgca ccatttatca tatttaactg
     1501 tgctgattat tctgataatc agcaattatt attagcgcag ctttttggat atgtaaaagg
     1561 agcatttact ggagctgata aagataagta tggtattgta catgaagcta ataatggagt
     1621 attgttttta gatgaagttc atagattatc tgcagaggga caagaaatgc tatttctgct
     1681 tatggataaa gggatatata gaaggttagg agaggctaat aaggtacacg agtgtagagt
     1741 tatgataatt gcagccacta cagaagaccc agaaacagca atgttggaaa cgtttttaag
     1801 aagaatacct gtaactataa agattccgtc tttagaggaa agaggctttc aagagagaat
     1861 gaagcttata tgttattttt ttaaagaaga gagtgttagg attggtgtta agttaaaggt
     1921 ttcaaaagaa gttattaagg catttatttt atataagtgt aagggaaaca taggccaatt
     1981 aaaaagcgat atacaattaa tttgtgcaag agctttttta gattatatga cctataaaag
     2041 ggaatgtgta tatgtaaggc tctctcttct accaaataat attagagaat cattatatgg
     2101 aaataataga aaagaggagt ttgtacaaaa tttcagtttt attggaaaag atgatattat
     2161 ttttttacca gaagaaaagg attttgaaag tgaaaattta ttaatagaaa ataaaaaata
     2221 tgatttagat ttttatggaa tgataaaaga aacttggaat aaacttcaac aagaaggtat
     2281 taaagagtca caaattagaa gtgttataga tggagacata cagaagtatt cctataattt
     2341 aatgaatact tttatatata ataatagtaa tcaaacagct tataacaaca ttgttaatga
     2401 ttcaattgca actactgtaa aatatatttt acaaaagcat gataaatgga gcaaaagaga
     2461 aggattagat aaattaataa aagctatagc cttacatatt cagaatttaa ttgaaagaat
     2521 aaagattgga aatatagtaa agcatcctaa taaagatgaa attatgaagg aaagagcata
     2581 tgaatataac atagcaaaag aaatattatc taacatgtca ttaatgtatg gagtagagtt
     2641 tccagaagat gaagcaattt ttttagcaac atttctttat ttatcgtatg tgggattaaa
     2701 tgaggagagt atagcaatat tagtcataat gcatggtgaa tctacagcat ctagcatggt
     2761 gaatgtagcc aatactttac tggattgtaa ccatgcagtg gcaattaata tgggattaga
     2821 agatagagtt caggatattt tagtacaagc tgtagaaata gccaataaaa ttgataaagg
     2881 aaagggaatt ttaattttaa ctgatatggg gtctatttta acatttgcta aagttattcg
     2941 tgatgctaca ggaaaagaag taaaagcagt agatatggta agtacaccta ttgtaataga
     3001 agcaactaga aaggctctaa ctccagaaat gacattagag aaattatatt ttaatattat
     3061 agaatctata aagaaacatt atggagatat ggaagttaca tatgctgaaa aagataatgg
     3121 tggtagatat tttgatagac tactaattga taatattagt aaaactttga cttttttaaa
     3181 tggggaaaag gcttatttta tattaaaaga agtaattaat agaattagtg aacactattc
     3241 tttagtaatt caagatgaac ttttagtaaa gtttatattt cattgttcat gtatgattga
     3301 aagagtaata ataaaagagt ctttagttta taaatcatat gaagaaagaa ttagtagaaa
     3361 taaagaatta tatttagtta taaaagaaag ttttaagcta gtagaagaga cattcgatat
     3421 aattatttca gatatggaat atgcaaatat attagatata tttgaatcac aatatgatac
     3481 agataaattc aaaagttgat acaaaattta aaaatagcca aaaggctatc ttttttttac
     3541 tttttttgat acagatttga tacaggttga aaaaatcttt tgatacatat cgaaataata
     3601 agtttttaaa aaaaattgag taaaaagttt tgattaaatc ctgtaaaata gccatttatt
     3661 attgatacac ataagttggc acggcaattg cttatataaa aagtgacaag aaaagtgagg
     3721 tgaaattggt gataggaata atagttatga ctcatgggag ttttagtgaa gaaattataa
     3781 aaagctgtga acttattgca ggaccagcag aaagaacagc agcaattaaa ctaaatagga
     3841 atgataatat tgaagactta aataaaaatt ttgtagaaaa gttaaatgaa ttagatgaag
     3901 gtgacggagt tttagtatta gctgacctat taggaggttc gccaagtaat gttgcaagtt
     3961 taaaccttaa aaaaggtggc aagtttcatg cacttactgg agtaaatctt ccaatgctac
     4021 ttgaagcttt aattaataga gagggaaaaa gtttagaaga gttagcagaa gcatgtattg
     4081 aagcaggaca aactggtata aacaatatta ataaagttct tagctcaatg taatagaaaa
     4141 aggagtttga ttaaaaatgt ctttagtaaa atattgcaga tgtgatgata gattaataca
     4201 tggtcaagtg atatataaat gggttaagca tctaggtgta aaaaagatag tagtagtaga
     4261 tgatgaaaca accaatgatg ttattgctaa aggtttgata aaaatggcag caccaaagaa
     4321 tattgattta tcaatattaa ctgtaagtga atcaaggaga tatttttata ataatcaagc
     4381 tgatgataac gtttttgtat taattaaaaa tttagatact gcaaatagaa tgatagaaga
     4441 agggattaat atcaaaaaac ttattgttgg aagaatacct acaggaatag gaaaaaagaa
     4501 aatatctcaa aatgtataca ttaataaaaa agagtttctt ttaatagatg aatttattaa
     4561 aaagaatatt aatgtatcaa ttcaaatggt acctgatgaa gaagaagtag aattaaacca
     4621 aaatattaat cgctataaag gagagtttat ttagcaatga ataaatataa ggtaattata
     4681 ctgtgccatg gaacttgggg aaaagtctta gtagaagaaa caaataaaaa ctttggattg
     4741 tcaaatcaat atgaagtttt atcactaagt tctgaaaaag atgtttctgt atttatgaaa
     4801 gaggttgaag aagtagtaga taaagaagat gtgaaaatta taatatcaga tttatatgga
     4861 ggatctacat caagtgttgc aatagctgtt gctataagaa gaggaataga tgcaataaat
     4921 ggacttagct tacagacaat tttaatcgta gatgaagaat taagcaaaga agataaatta
     4981 catacattac cagaaagaat cgtcaaaaaa aataataacc tttgtgtgga tttaattaaa
     5041 gaatttaaaa aaatataaat tatgaggtga atataatggc aaaaataaca ttagtaagag
     5101 tagaccacag attaattcat gggcaagtta taacaaaatg ggtaaaaatc gctcaagctc
     5161 aaaaaataat aatagttgat gacttcttag gtcaagatga atttatggct gacatatata
     5221 aaatggcagc accatcagga gtagaagtag ttattttaac agcagaagat gcgggacaag
     5281 cattccaaaa caacacttta ggagataaaa atatatttat tctatttaaa aatgttgata
     5341 tggcaaataa ggcttataaa gctggactta aatatgaaaa gattcaatta ggaggtattc
     5401 caaatgaagc aggaaagaaa atggtgttta cagctgtttc attaggaaat gaggatgtag
     5461 aacaacttaa tgaacttaat gaaaatgggg ttgaaatagt cctacaagtt ataccagagg
     5521 aatcatctat gacatatgaa aatgcgttaa agaaatttaa ataaaaaata taaattagga
     5581 ggaattataa atggaatctg ttttgatgtt ggcaattgta acaggattat ggtattggtt
     5641 tgcagcaggt cttgcagggt ataccctttt ctcaacacta aagtctcctt tatttatagg
     5701 ttttagcctt ggattacttt ggggtgatgt aacaactggt atgattgttg gtgcaagtat
     5761 agaaatggtt tatctaggaa tggtcgcagc agggggaaat attccatcag ataaatgtct
     5821 agctgctctt attgcaatac cagtagcact tcaaactggt gttaatgcag aagttgcagt
     5881 atctattgcg gtacctttag gtgttatagg agtattagtt aataacttaa gaagaactgg
     5941 taatgctgta ttggttcata aggcagataa gtatgctgaa gaaggtaata caaaaggcat
     6001 ctggagatgt gcaacacttt attctttaat atttgggttt gttttaagat ttccaatagt
     6061 ttttgtatgt aacttttttg gagcagattt agttcaatca ttattagatg ttataccaca
     6121 atggttaatg aatggattaa cagtaatggg cggtatatta cctgcattag gttttgcaac
     6181 aacaatattt actataggta aaaataaatt cttaccaatg tttattatag gattttttat
     6241 ggttcaatat tttgaaatat caattactgc agctgcaata tttggtgttt gtatagctct
     6301 tttaattacc tttatgaaag aagacaaaag ggtaggtgaa gtataatgca agaaataaaa
     6361 aataaagaag ttaaaaatgg acaaggtcca actgaagtta ataagacaag agtactcaca
     6421 aagaaagatg taacaaaaac atacctaaga tggtggtgga cagcagagtt atctaattct
     6481 tttgaaagaa tgcaagcatt agcagtttgt gcaagtttca ctccggcttt agaaaagtta
     6541 tataagaaaa aagaagattt agtagatgca ttaaagagac acttacaatt ttttaatact
     6601 caagctatat ggggaggatt aattcatggt acagttttag ctatggaaga agagaaagct
     6661 actgaaggaa aaatcccagg agaagttatt tcaggtgtta agaatggtct tatgggacct
     6721 ttggctggaa ttggagatac tttagatttt ggtacatttc aaacaatatt cttagcttta
     6781 ggtgcttcat ttggagcaga aggaagtgtt ataggtgcat ttttcccaat aatgtttagt
     6841 atattactat tttgcgaagg ttattactta tttcatttag gatattcttt aggtagagat
     6901 tcaattaaga aaattctatc aggtggtatt gtcaataaga taattgatgg tgcttcaatt
     6961 ctaggaatgt ttatgatggg agcattatct gcaactacag ttaagctttc aactccgcta
     7021 tcatttgata ttggtggtaa agcaattgtt gttcaagata cattaaatat gattgctcca
     7081 ggtttattac cacttggtgt tgtattcttc gtatattggg gaatgaaata caagaagtgg
     7141 acaataacta agttattagt aattttagtt gtattagcat tggtaggatc attcattgga
     7201 atattctaat tcacagtgat taaaatatag aatttaattt taggaggaga aagaaatgtc
     7261 agaaaaaatg tatacagaaa aggaatattt agaaggaatt agaggagcaa caggagaaag
     7321 agcagtatgg ttctatcttc ttatgaaaga agctgaaaaa ttaggagcta atccagatga
     7381 tatatgtaaa gaagcaatat atggatttgg taaaatgaga ggtcaaaaat ataacgtagc
     7441 agatactcca ggaaaaatgg cagaaatgtt atataacagt aaaggacaaa aagtgtttga
     7501 aatggaatta gtagaaaata cagatgagaa tggcgtttta aaattccatc actgtccatt
     7561 agatgctgct tggaaagaat atggattaac taaagaagaa agaaaagaaa tttgtagatt
     7621 agcttgttat ggggattatg gaagagttga ctgtgcacaa ggtgttaaat tagattttgc
     7681 tcaaaaatgt gctcatgatg atgaagtttg tgaattagta tttacaaaaa aataattttt
     7741 aatgctaaaa gcctgttctt taaggacagg ctttaaaaag atagaaagta ggttgattta
     7801 tggatataag cagaagtgta gataatagtt taaatagaac catagaattt ctaaaagaat
     7861 tgataaaaat tgattcacaa caaggtgaac ctatttcaca atgtcctttt ggtataggac
     7921 ctaaaaaatc tttagataaa actttagatt actgtgctag tttaggtttt agtgttaaaa
     7981 atattgataa ttatattgga tatgcagaaa ttggagaggg agaggaatta atagggattc
     8041 caatgcattt agatatagtt ccaccaggag aggggtggag tgtagaccca ttttcgggag
     8101 cagtaattga caatattatt tatggaagag gtgtcataga taataagggt gctgtatcta
     8161 tgcttataca tgtattgaaa aacatagagg atatgtatcc tacaattaat aaaagaataa
     8221 gattaatttt tggtacaaat gaggagacag atatgaaatg tatcaaatat tacttagata
     8281 aaggtgaaga aattccaagt atgggattta ctccagatgc tatgtatcca gtggttaatg
     8341 gagaaaaagg aagggttcat ataaggatag aaaaagaaat taaaattgac aaaagtaaac
     8401 cttacataat agtaagggga ggaactaaag aaaatgttgt tccatctcat tgtactgcaa
     8461 aaattattaa tggaattatt tcagagttaa ctactaaagg agtagctgta catgcaagta
     8521 atcctgaaaa gggagaaaat gctatatcta aaatggtaat aaagatagtt gaggataata
     8581 tggattttca gcatagagaa gatatagaat tagtttcaaa atacctttgt agtgattatt
     8641 atggagatgc attggggatt aatcaatatg atgaggtatt caaaaacact accttaaatt
     8701 taggaatatt aaaagtaaat gaggaaaaaa tcgtatgtga gttagatata aggtatggta
     8761 aaaatatagt tttaaataac ataatagata gattcaaaaa ggttttttgt aatggttgga
     8821 aaatagaggt tattgctcat aaagacttac attatgtgga tgagagtaat ttagtactta
     8881 aaaaattatt agaagcctat gaagaagtaa cagatgaaaa tggatataca attgctatgg
     8941 gtggtggcac atatgcaagt tggtttaaag atatggtggc gtttggacct aagttcttag
     9001 cgtataaaac tggtggtcat ggagtagatg aaagagtacc aataaatcat atacgaaaga
     9061 atatggagat atatactttg gctttaataa aactattaga attgtaaaag aagaagagac
     9121 tttatgtgca aatttacaga tatcttaaaa agagagataa aacgagttca actttattta
     9181 aatgttcaac accatcaaat ggggctgtct caaaatagag ataagcctct tgcttattat
     9241 tgaaatgatg aaaatatatt ttacttcata caaaaaagag tgccttatga agtaaaaagt
     9301 taattttgag acaccctcat ttgatgatgt tgaagagaaa ataagtatga aattattaaa
     9361 aaaagatgat tttataaaag ttaaggttga aaataaaagt taattgaatg gaattagata
     9421 aatggagaag gctgtatact atctaataaa ataacaaaag agtgttataa aactggttat
     9481 atgattagag aaaagtctat tgatacttat ccagatagtg gacggggatt tatgacttgg
     9541 gatgaaaatg gagaataata aaaattatag tttatacaat ttaataagat atatgtacaa
     9601 gcagttaatc taaatgattg actgcttttc ttttatagat ttttatataa tagacaatta
     9661 ctaatacatg atagttgcct gttataatta gaggaataga tacgaataat aactttataa
     9721 aagaattaaa tggaaaattg aagagttata ttcaggattt taaaaagaat atgaaatgag
     9781 aaatgaaaat aaggtgttta tattaaaaga ggttttacac aaaatactta aaaacgtgat
     9841 aaataaaaac tgtatttaga taaattaact actagtttta tatataatat ggattttgtt
     9901 tacaaagttt acatatttca caaaatattt ttaaaatata tttaaaaata tattttatat
     9961 acttgttcgt ttgtttctct gtggttgatt ttttcgtcaa aagtcaactt caaatcgctt
    10021 gttagcttta catgtttaag gatataatta tacataagga gggattgaca tgaatgaatt
    10081 aaacatcgca aaaacgctga ttttaaagcg taaagaaaaa ggaattacac aagatgaact
    10141 tgcaaattat ataggcgttt ctaaagcttc tgtatctaaa tgggaaactg gacaaagcta
    10201 tcctgacata acctttttac cgcaactcgc gacatatttc aacattaccg ttgatgagtt
    10261 aatatgttat gaacctcaaa tgatgaagga agatattaat aaactgtata ataaactgtg
    10321 caaagacttt acggttaaac cgtttgatga agtcatggat gaaataagag aaatcataaa
    10381 aaaatattat tcatgttttc ctttgatttt tcggatgggt cttttaatag ttaatcacta
    10441 tgatattgta gatgaaaaaa agcgtgaact attaattagt gaagcattag aaatatttat
    10501 tcgtatacag gaaacttgta atgacataga tatttgccgt caagcaaaaa gtatggaggc
    10561 aacttgttat atattgttaa atcaacccat acaagtgatt gatttattgc aaaacagcaa
    10621 ttttcccatg ataaatgaat ctatacttct tgcacagggg caaatgatga atggtcaaat
    10681 ggatgaagca agagaaacat ttcaacttgg tgcatatcag aaccttatat cattagtcca
    10741 aaatctagtg ggtattttgc aaaatgcaga taaattacaa atgaaggaaa tagaaagacg
    10801 tattctagca atttcagata tatttgagct tgatacatta tctcctgcta ttatgctttc
    10861 ttcatattta acacagacac aaattaattt aatacatggg gataatgaag gagctattaa
    10921 atcattacga aaatatgtag atttagcaac acgtgatata tacccaatta taatacatgg
    10981 ggatgacttt tttaataaac ttgacagatg gatttctgaa attggaacag gtattacacg
    11041 tgatgataca atcgtaaaag caggtattgt tgctgctatt aaaaataatc caatgttttc
    11101 tttattaagt gaaaacaaag aatacaaatt tcttatagaa aaattatctt tattggagga
    11161 ataagattat ggaaaatttg atggaatatt taccactttt gattcctgtt attattttag
    11221 atttaatact tataattact gcacttgttc atgtattgag acatccaaat tataaaattg
    11281 gaaataaggc aatatggatt attgtagtac tgtttattag tctaatagga ccaatcttgt
    11341 attttacaat aggaagaggt gaagaatgat gaatattgtc tcaataaatg gattatcaaa
    11401 aggttttggt aatcgaaaga taatagataa tctaaatttt actgtaccag aagggtcagt
    11461 ctttgggttt gtaggtaaga atggtgcagg taagactaca acaatgaaaa tggtacttgg
    11521 gttacttgaa ccagatagtg gaactataga tgtttgtggt gagaaagtga catatggaag
    11581 aacaagttct aatcgtcatg taggttatct gcctgacgtt ccagagtttt acaactatat
    11641 gagaccactt gaatatcttt ctctttgtgg tgaaattacg gggctttcaa aaaaagaaat
    11701 aaagataagg agtgaagagt tgttatcact tgtggggctt ggcaatgaaa agagacgaat
    11761 aggtgggttt tctcgtggaa tgaagcaacg acttggtata gctcaagctt tgctttcacg
    11821 tcccaaatta cttatttgtg atgaaccaac aagtgcactt gaccctgttg gtcgaaaaga
    11881 aatattagat attatgctta aaatcaaaga ctcaacaact gtaatatttt caacacatat
    11941 actatcagat gttgaaagaa tctgtgacca tgttgctata ctaaacaaag gaagcattgc
    12001 tcttagcggt acactttcag aaataaaaag tatgcacgga aaggatagac ttttactgga
    12061 gtttgcaagt aataatgaaa ttcaaaagtt taagtctagt ggtggcatta aatccttact
    12121 aaaagattca gaagaaacta atatggaaat tgttttgcat ggtaaagata taaaagcaat
    12181 acaaaaaaca gttatatcta cactcgcaga aatgaatctt tgtccagtaa agatggaact
    12241 aatagaattg tcacttgaaa atttgttttt ggaggtagta aagtgagaga atacatagca
    12301 tttacaaaaa aagagtttaa ggagaacata agaaattata aattacttag tttgactatt
    12361 ttatttttag tatttggaat aatgagtcct ctttctgcaa aatttatgcc agacttgatt
    12421 gctcattttg ctccaacttt aaaagttact actgcaccaa ctgctttaga ttcttggaca
    12481 cagttttctg gaaatatttc tggtcttgga acgagtttaa ctctaattgt tttttgcaat
    12541 attttatcaa acgaatattc taaaggaaca ctcgttatta tgttgactaa agggttatca
    12601 cgttcatctg tagtattgtc taaattttct attgcagtaa ttattatgac aattggtttt
    12661 tggttgagct ttttatgtac ttatggatat acaatgtatt tttggccaac tgcaaatctt
    12721 aatcatatta tattttcagc atttaactta tggttaatta gtattatgta tatttccata
    12781 ttaattcttg ggtgtgtact atttagacca gcatttgcaa gtgtgttact tgtattagca
    12841 gcaactgcta ttttgagttt gatttctatt cctaaacaga ttgcacctta tacgcctaat
    12901 ttcatattat caaaaaatat agatttagtt tcaggtaaag tgacacctcc agaatttatt
    12961 attccaatca ttgtaacaat tgttatttcg atagtatgtc tgttattagc tgttgttttg
    13021 tttaataaaa aagcagttta aggatatttt tattataaat acaaatagtg ctgtattgtg
    13081 ggaaaatgtg tttagtattg tagaagaata tatttaagta aaaaagaggc tgtctcaaga
    13141 tagagaaata agcctctttt ttattttagt cctcctcact atactttata ataattaagt
    13201 tattacctta aggaactatc taaaaaaaga gatatagatt ataatctaat gcttatcagt
    13261 tatttgaggt gtttagtata tagagtctaa agtagtgaag gactaaaata atattgtttt
    13321 cattaaataa agataagaat ctttaattgt ggtaaaatat actaaaaaat cattttaaaa
    13381 gtgataaaat tattttaagt aaatatttta aaggggtgta gatatggaaa agtcaaaagt
    13441 atatttttgt gatttatact caaattcgca aaataaaaac gtaccaaaca atgtaagaag
    13501 attatttgat gaagcaggat tcaaagattt aatagaaaag aatgaccaag tcgcaattaa
    13561 acttcacttt ggagaaaagg gaaatactac atatatgagt cctgtggcag ttagacaagt
    13621 ggttgataag gttaaagact gcgaaggaaa gccattttta acagatacaa atacactgta
    13681 tacagggagt agaacaaatt ctgtagacca tcttacaact gctatagaaa atggatttgc
    13741 ttatgcagtt gtaaatgcac cagtaataat tgcagatgga ttatatagta gaaattatga
    13801 gaatgtaaaa atagacaaaa aacattttga aagtgtaaaa ataggtggag aaatttataa
    13861 ttcttctgca atgatagtaa tgagtcattt taaaggtcat gaagcagcag gttttggagg
    13921 agcattaaaa aacttagcta tgggatgtgc aagtgcagca ggaaaacaaa tgcaacattc
    13981 tgatgttact ccagtagtaa aggaaaagaa gtgtgtaggt tgtggaaagt gtgttaattc
    14041 ttgtcctaca aaagctataa gtatagtgga taaaaaagca gttatagatt ctgatgtatg
    14101 ttatggatgt ggagaatgtc caactgtctg tccaactaga gcagtaacta tacaatggga
    14161 aagtgactca gatgtgtttg tcgaaaagat ggcagaatac gcttatggag cagtatcaaa
    14221 taaaaaagac aaggtagggt atataacttt tgttatgaat gtaactccac tttgtgattg
    14281 tgtaccttgg tcaggtagac caattgccca tgatatagga atattagcat caacagaccc
    14341 tgtagctatt gaacaagcat gctatgattt gatttgtaag gaaatgggtc atgatgtttt
    14401 caaacatgaa catcctcatg taaatggaac tagaataata gactatgcat gtgaaatggg
    14461 tatgggaagt aaagaatatg aattaataaa attataagaa tagtagcact taaatgcata
    14521 taattattgt aagagaaatc taaaagtatc aaaggagtat gcatttatga aaatttacta
    14581 tgagaaggaa aacagcaaga aggctaatgt aaacaatatt ccaagtgtat ttgcgtttaa
    14641 ttttctaaga aactttttga ctagcaaaga gtatgaggaa actagaaaag aatattttat
    14701 aaataatcta acaaaaagtc aggttaatca agccatgaag gatttaaaat ggctatttag
    14761 agaatatgaa ggcttagagg ttattacaat ggaaagcttg gatggagtta agacaaggat
    14821 agttttataa accagtaaga attatattct aataaaaaat actgctttaa taataatatg
    14881 tataatctat atactaatat tattttgaag cagtattttt ttgtgtatag taaacttgtg
    14941 tctaaaataa agttgaccaa taaatgttat atatgcaata ctatatataa taagtaaact
    15001 acataaaaat attaagttga caaataggag gatatatgga aaaatacata ataaaattaa
    15061 aaaataaagt attgagagaa aaagaaataa gttatgaaga agctcttaat ttaataagcc
    15121 ttgatacaaa taataaaaac gattttgata ttctattaaa atctgcaaat gaaataagag
    15181 aatattttat gggaagaaaa gcagatttat gtacgataat gaatgctaaa tctggcaaat
    15241 gtagtgaaga ctgtaaattt tgtgctcaat catctcatta taaaactggt gtagaagaat
    15301 attcattatt agattacaat gaaatcttaa atagagctaa agagatggag agtaaaggag
    15361 tgcatagatt ctcattggtt acaagtggta aaggtatgtc aggaaaggag tttaataata
    15421 ttttaaatat ctatgaagga ttgagggaaa atactaattt aaaattatgt gcatctttag
    15481 gtattataga ttatgagcaa gctaaaatgt tgaagtcagc aggtgtgact acatatcacc
    15541 acaatgttga gacgtgtaga gataattttc ataatatatg tacaacacac acatataaag
    15601 ataggataaa aactataaaa gatgctaaaa aagctgggct agatgtatgt gtaggcggta
    15661 taattggaat gaatgagagt gaagaacaga gactaaaaat ggtttttgaa atacgagaat
    15721 taaatgtaaa atcatttcca ataaacattt taaatcctat taagaatacg ccaatggaaa
    15781 attatgatgt attagaacca atggaaatac taaaaacaac agcagtattt aggtttataa
    15841 ttcctaatgt gtacataagg tatgctggtg gaaggctatc attaaaggga tatgacaaag
    15901 ttggatttaa tgggggagta aattctgcaa ttgttggtga ttatttgaca actgttggaa
    15961 gtgggattga aaatgataaa aaaatgatta tagagcaagg atttgaactt taataaatac
    16021 tacttaaatt aattaatata cttttagata attataaaag tatattaatt ttttttgcag
    16081 attaatttaa atattaaaat tgaattataa gttgcaatat tttgaaaaaa gggaaaaata
    16141 tcaaaattta tataaaatgg ttgcaaatgg gaaaaggatg tgatagtatc taggtaaccg
    16201 gttaagtaaa acggtttcct aaattttggg ggtgaacttc ttgagtataa ctataaaaga
    16261 aattggcgaa ttggctggtg tttctaaaac aactgtgtca aaagtcatta ataataaaga
    16321 tgaaaatata agtcaggcaa caagagaaaa aattttgaaa ataatgaagg aaaaaaatta
    16381 tgtaccaaat aaattagcac aaagtttagt aactaaaaaa actaatacta tagggcttct
    16441 tataccagat atacgaaatc cattctttac agatgtatca agaggtgtag aagacaaagc
    16501 taatgaagaa ggctacaata taattttatg taatacagat gaagatgcta aaaaagaata
    16561 tgaaggaata agaacactat cagagaggat gatagatgga attatatttg cagcatcttc
    16621 aaatacaaac tggaaagaag caaattataa agatataaaa ataccaactg tcttgattga
    16681 taagaagata agtatgaaca aagaaacctt aaaaggaatt gttaaaataa ataactttga
    16741 aggggcctat atagctacaa aacatttatt agatattgga aataagaaaa taatatacct
    16801 aagtggtcca cttcaaaatg aaatagcagt agatagatta gaaggatata aaaaggctct
    16861 aatcgagtgt aatttaagct acaattctgc ttatgtattt gaaggaaaat ataagattga
    16921 atggggacaa gaatttataa aaaatttaga aaagatagat tttgatgcaa tattttgtgc
    16981 aaatgatttg attgcaatag gtgtcataag aggtcttaaa gaaagagggt taagtatacc
    17041 aaatgatgta agtgtcgtgg gatttgatga tattcaaaca tcaagtctaa taagcccttc
    17101 tcttaccaca gtaaaacagc cgtcttatga tataggatat aaagccagtg aaatattgat
    17161 aaattgttta agaggagata aaaaagaatc atttgatgaa ttgattttca aaccagaatt
    17221 agtcataaga gattcaacaa aagaaagtga ataacagcac gagagacata aatagatatg
    17281 taaaggagta atgtaatggg aaacatagtt gtcataggaa gtgtaaacat ggacatggta
    17341 tgttctgtag ataaaagacc agaaaaagga gaaacagtat taggtaatag tttttttaca
    17401 tcacctggag ggaaaggggc taatcaagct atctcagctt ctaaactagg agcaaatgta
    17461 aaaatgatat catgcatagg ggaagatggc ttaggagagg agttaataag aaattttaga
    17521 acgaataaag ttgattatag tttagtatcc agaaataatc acaaaagttc tggtgttgct
    17581 gttataacat tatgtgaaaa tgataatagt attgttgttg taccagggac gaatgagcta
    17641 gtagatatag aattaattaa aaagaatgaa gaagagataa agaatgcaga tatagtattg
    17701 ctacaattag agattccatt aaaaacaata aattatgtag tgaatttctg ttttgaaaat
    17761 aggattaagg ttttattaaa tccagcacca gcagtaaaac taaatgaaga tataatagaa
    17821 aaagtaactt acttaacacc aaatgaacat gaatataaga tagtttttga cacaaatgaa
    17881 gggatagaag aagtattaaa aaaatatcca aataaacttg taataacaga aggaaaaaat
    17941 ggagctagat tttatgatgg tgaggaaatc aagcatgtat cttgtataag tgttgatgtt
    18001 caagatacca caggagcagg agatacattt aatggagcat tggcagtggc tataacagaa
    18061 gggaaaaaat tatatacagc agtagaatat gcagtagtag tatcaggtct ttctgtaact
    18121 aaactaggtg cacaatctgg tatgccttat aaagaagatg ttgaaaaata tttaaataat
    18181 aaataaaaat ctaatttgtt caaatttaaa actattaaaa atggaggtta attatgagag
    18241 tgagaaaaat tttatcttta ggaattgctg gaatactagc aataggaatg ttaacagggt
    18301 gttcaatgga aggaccaagt aaatcagata ataaaggtgg ttcagacaag aaagatttaa
    18361 ctattggagt atctacaata actcttcaac atcaattttt tatagatatt gatgagggaa
    18421 taaaagaaaa agctaaggaa ttaggtgtga aagttatagt aaatgaccca gaccaagatg
    18481 tagctaagca aacatcagca atagaagatt ttatacaaca aaatgtagat ggaatgatag
    18541 tacttggaac tgataattcg gctatagtac cagcagtaga aggagcattt gaaaaaatgc
    18601 cagtagttac agtagatgct gtattaaata ctgaaaatat tacaagttat gtaggaacag
    18661 ttagttatga tgctggtaaa aaattagggg aatacactaa aaaatacata gatgaaaagt
    18721 taggtggaaa gtcagaaata gcaatagtga cagatttaaa atctcaaata caaatgcaga
    18781 gaatagatgg atttaaagat gcgttaaaag gttcagcaaa tgttaagata ttaaattcac
    18841 aaccaggata tgacagagaa gaatctctta atacagtaga aaacttaatt caatctaatc
    18901 cagatgtaga tataatatat gcaacagctg aaaatagtgt actaggagct aaagctgcac
    18961 ttgaatctgc aaaaaataag gatgttaaga ttgtaggatt tgacttaact gaagaagcat
    19021 caagtggaat aacagatgga actattcttg ctatgattca acaacaacct aaagagatgg
    19081 gacgtttagc tgtagaagca gtagtaaaag ctataaaagg ggaaaaggta gaaaagaata
    19141 taccagtacc agctttactt tatgataagg aaaatataaa agactttaag aattagtaaa
    19201 ataaaatgag attatttgaa agaaatccta tttaaaaagg agtgtcatta tgagtaatat
    19261 aattctgaag ttaagtaata ttgctaaaga atttcctggt gtcagagcat tagataatgt
    19321 taattttgag ttatttcatg gcgaagttca tgcactactg ggtgaaaatg gtgctggtaa
    19381 atcaacaatg ataaagattt taactggtgc acattcgaaa acttctggaa aatttatttt
    19441 tgaaggcaaa gaaatagaga atatatcccc agatatctct aaaaagatag gtataaatgc
    19501 catatatcaa gaactaacag tttttgacga attaacagta gcacaaaata tttttatggg
    19561 aaaagaaata aatggcaaag tcttaaccaa tgacaaaaaa atgaatgaag aggctaaaaa
    19621 gattttcgat aatatgggaa tagatataaa tccaaatagt ttagtcaaag agcttagtat
    19681 agctcaaaaa caaatggtag aaatagcaag agttttatca tcagaaacta aagtattaat
    19741 aatggatgaa cctacttctt caataagtaa aaaagagact gaaatacttt ttagacttat
    19801 aaatgattta aaagagagtg gagttagtat aatatatata tcccatagga tggaagaact
    19861 ctttgagatt tgtgacagaa taactataat gcgagatggg aaaacgattt cgactttgaa
    19921 taccaaagat gtatcgtctg aggaagaatt agttaattta atgatagata gaaagctaga
    19981 ccaatttttc ccaaaaagaa aagtagaaat aaaagaagaa attatgcgag tggaaaatct
    20041 aactaagaac aatgttttta atgatattag ctttaatata agaaaaggtg aaatattggg
    20101 aataggtgga ctagttggaa gtaagagaag tgaaatagta gaagctatat ttggacttag
    20161 aacatatgat tctgggaaaa tatatttaaa caatgaagaa gtaaagttta aaactccatc
    20221 agatgcaata gaaaatggac tgggattaat aacagaagat agaaaaggta ctggattgtt
    20281 tttacaaatg agtgtcaaag aaaacacaac tatggctggg ttaaaaaaga tatctaagtt
    20341 taaatcaatt atagatagaa aaaaggaaaa agagatttta gaaaaataca tagaagcttt
    20401 aaagataaaa acaccacata tgaatcaagt tatacaaagt ttaagtggtg gaaaccaaca
    20461 aaaagctata atagcaagat ggcttttatt acaaccagat atattgatta tggatgaacc
    20521 aaccagaggt atagatgtaa atgctaaggc agagatatac aatctaatgg gagatttagt
    20581 ggagagtggt gtaagtataa taatgatatc ttctgaaata ccagaattaa tatcaatgag
    20641 tgacagaata atggttatga gagaaggtca tataagtgga tttttagaag gtgaagaaat
    20701 ggttgaaaat aatgtattaa aattggcttt cggaggtaaa attaatgagt tcaataacta
    20761 agcataaaga agaaaaatct tctccattat tgacaaaatt aacaagtgta tttagagacc
    20821 aagggagtgc agctgtagga cttgtaataa tttttataat aatgagtgta gcttcatcta
    20881 actttttaac tttagataat ttaattaatg ttggaagaca aatatcaata aatgcaatac
    20941 ttgcagtagg tatgacattt gtaataatta caggaggaat agatttaagt gtaggagcag
    21001 taattgcttt agtaggtaca ttttgggcta caacagtagt gaattataat gcacctatat
    21061 gggtaggtat gatacttgca ttagcaatag ggacaatatt aggagtcata aaaggtgcaa
    21121 ttatttcaac acaaaaacta cctccattca ttgtaacttt agccatgtta acgataataa
    21181 gtggggcaag ctttgtattt acaggaggaa gacctatatc agtaaataca gatgcattta
    21241 agatgctcgg tagaggatat attggaccaa taccaatacc agttataatt atgattatag
    21301 ttgtaatagc aggacatttt ctattaaaaa gaactaattt tggacgtcat gtacatgcag
    21361 taggtggaaa tgaagaggcc gctagattat gtggagtaaa agtaaataaa gttatagtaa
    21421 aagtatacgc actagctgga ctactaacag cattagctgg gataatacta tcatcacgtt
    21481 tggcatcagg ttcaccaact gttggagatg gagcagaatt ggatgctata gcagccgttg
    21541 tgcttggtgg tactaatatg atgggtggtt ctggttctat agtaggtaca tgtataggtg
    21601 tcggaataat aggaatatta ggaaatggat taaacttatt aagtgtatct tcatataatc
    21661 aaatgattat aaaaggtttg gtaatgttat ttgctatatg gataaataat ataaaattaa
    21721 aaaaatcagc taaatctaaa tagtaattaa attttagctt ataaactata aaaattatta
    21781 agtttttgta tatattttta tactttaatt tgcatacatc tttatacatt gaatgggaat
    21841 atgctaagtg aaaaagttta ctatggctta aatagatgag attagacttg aaattttaaa
    21901 tgtaaaaata aatcttagga ggaaatatga aaaaaattct cttagataca ttaaatagta
    21961 aaaaacaaga atacatagat tatttaaaag aacttgtaag tataaagact gaagatgttg
    22021 gtcatggaat actaggtgga tttgaaaaag aaggacagga atatattgaa aaacttgcaa
    22081 attacatagg ttttagtgta gatagacaag aaatgagcga agaattaatt aaaaaagcaa
    22141 aaaatatata taaagaagga aatctaggtc acaattatca agatagatat aatttaattt
    22201 gtaagtatag tgatgattta cctggtaaga caattgtatt taatggtcat gtagacacta
    22261 tgccaccagg tgatataagt aagtggaaat ataatccata tcgtgctaca gaagataatg
    22321 gaaaacttta tggtcttgga actgctgata tgaaatctgg acttatagca tcaatattag
    22381 ctgtaaagct tataaaagat agtggtctaa atgtacctgg taatgtaaaa ataatgtcag
    22441 tagtagatga agaaggtggc ggaaatggta ccataaatgc agttatgaat ggtattggtg
    22501 gagattgttg cataatttgt gaaccatcag aacaaaactt aatagttgca catatgggat
    22561 ttgtattctt tgaagttgag gttaaaggtg tgtctcttca ctgtggtagt aagtgggagg
    22621 gtgtaaatgc aattgagaag gctatgttgt tgcttcaaga tataaaagag ttggaacata
    22681 actggcttat gatatataag catccactat taccttcacc tacaataaat ttaggtgtaa
    22741 taaatggtgg aacagcaggt tctactgtgc cagataagtg tgtatttaat ttatgtgtac
    22801 acttcttgcc aaatataatg agttatgaac aagttgtaaa tgatgtaaca aatgtcataa
    22861 tgacaagggc taatggagat ttatggctta aagataataa accaaatata aacatatatc
    22921 aatcagggtt aggttttcag atggacaagg attctgattt tgtagtgaat gcacataaaa
    22981 tactagaaga aactttagga aaaaaattag agataaaagg ttcaacagct ggcaatgatg
    23041 cgagattaat gaaaaattta gctgaaatac caacacttat attagggcca ggttcaatag
    23101 aacaatgtca ttcaattgat gaatatgttg aaattaagga gtatttagat agtatactta
    23161 tgtatgcaag tctaatatta aacttataaa agtaggagga atatataaat gaaaaaagta
    23221 ttaatagtag gggaatcttg ggtaaaaaat ataactcata taaaaggatt tgatacgttt
    23281 gttacaactc attatgaaga agctgtaaag tggttgaaag aagcaataga atctggagga
    23341 tatgagacag ttcatatgcc tgcacatgta gcagcagata gttttccgta taaattagaa
    23401 gagttaaatg agtatgactg tataatatta tctgatatag gttctaatac gttcttatta
    23461 tctaatagta cttttataga ttgcaatagt aatccagata gacttgaact aatcaaagag
    23521 tatgtaaata atggtggggc gttaatcatg gtaggtggat atatgtcatt tacaggaata
    23581 gatgcaaaag ctagatttgg agaaactgct atcaaagatg tattaccaat aacaatgata
    23641 gataaagatg atagagttga aaaaccagca ggaataattc cagaagttat tgactctgaa
    23701 catccagtat taaaaggtat accaacagaa tggccaaagt ttttaggata taacaaaaca
    23761 gtagctagag ataattgtcc tgttttagca actattggag gagacccatt tgtagctgtt
    23821 ggcgagtttg gaaaaggaaa atctgctatc tttagttctg actgtgcacc tcactggggt
    23881 ccaaaagaat ttactgattg gaaatactat aataaattat gggttaatat gcttgattgg
    23941 ttaacatgct aaaaatttta taaaacttga aaacctctta cttgataaaa tgaaacttac
    24001 aagtcaagag gttttattgt ataaaatggt tgtatggtag aaaatatttt taaatcagtt
    24061 aaaattagtc attttggctg ataatcgata agttattaga aacagggagg tttatataat
    24121 taatatggat aatttaaata aaaagttttt gatggaatat atagatgaga tacagggaga
    24181 attaagagat atatcagata agatatggaa aaatccagaa cttcagtata aagaatacta
    24241 tgcttcaaat ttacaaaaag aatgtttaaa aaaacatggt tttagtatag agggattaga
    24301 aggtatggat acagcatttg tggctagtta tggagaaggt agaccagtta tagcagtttt
    24361 gggagaatat gatgctttag atggtttgtc tcaaaaagta tccactacta aagaacctat
    24421 tgtagatggt ggtgcaggtc atggatgtgg acataactta cttggtacag gttcaatagg
    24481 agctgtaatt gcagtaaagg aattaatcaa actaaaaaag ctgaaaggga ctataaaata
    24541 ctatggatgt ccagcagaag aagatttatc tggaaaagta ttgatgataa aaaaaggttt
    24601 ttttgatgga attgattgtg cttttagttg gcatccattt gatataaata ctccgataag
    24661 aatcccaaca cttgctaatt actctgtgaa atttagatat aatgggatta gtgctcatgc
    24721 agcacaagct ccttataacg gaagaagtgc acttgatgca gtagaactta tgaatatagg
    24781 ttgtaattat ttgagagaac atatatttga taattgtaga gtacactatg taactactaa
    24841 tggtggaaaa atgccaaata tagttcctga ttttgcagaa gtgtggtatt acataagagg
    24901 agttaaaatg gagcatgtaa gagatgtatt tggtaggatt gttgatatag caaaaggagc
    24961 agcattaatg actggtacta ttatggaata tgacattata agtggagtat atgactatat
    25021 tccaaataca atattaacag atattctatc acaaaatatg aaattagtag gtgtgcaaaa
    25081 ctataatgaa gatgactact attttgcaga taaattagct gagacagtgt ctatagataa
    25141 aagaagtagt gtttctagtg tattaagtgg aaataaggat attactaaga tgaatttaca
    25201 tgatgaggtt acagatgata catttactca taataactgt atatcattat cattggatat
    25261 tggagatgtt agctacataa ttcctacagc acaatgttct tgttcagttt ggcctattgg
    25321 tatttctgcc catacatggc aatcttgtgc cagtgcaggt tctgatatgg gatttaaggc
    25381 aatgttattg gcatctaaat ctatatcatg ttcaataaat gatgtaatgc ttgatgaatt
    25441 ggttataaat aaagcaaaaa aagaattgaa ggatactgta ggtagttttg aatatactcc
    25501 aattatttga agagtaattt aatacaagta agattgaatt gtatgaatta ggatgaatat
    25561 ataaattaat attattttta acttaacttt ctaattattt tcaatctaag atacaataat
    25621 tagaatatat ttttaaagtt tcctatttaa ggaaaataat ttgattttat gtttgataaa
    25681 aggacttata atttgtaagc ccttttatta tatttatttt ttgtttataa ttaattttat
    25741 tttttaggtt tagttaggtt atagattacc ctgaaaaata acatttaact tattattaaa
    25801 aacatgtaag aatagtttat aaatatttta ttttcgatgt tgtttattat atccatttta
    25861 tcgatggatg aggaatggct ctgccttccc tagttacaga tgtagtaggg aaaccaattg
    25921 cacaggcatc aaatccatat tcacctgttg gtactcctaa aatatcacaa aaatctttat
    25981 tatcgctcaa tcttcctatt acactaacaa gttcaaaacc taatcctaaa gcagttgatt
    26041 taagccacat attctgcatc acatgagata atgacttttg ttctataggt ggtattccac
    26101 gacgttctgc cacaataata agataaggtg ctgttcctat accaagaacc cctttttcaa
    26161 cttgcatttt taaggaagta atgaaggatt gaagggtaat gttgttcttt gccttcttct
    26221 cattgactga tatttgagtt ttaataacat caacaagaat ggtttttatt ttattcattt
    26281 gtaaactgtt atttgaaata acaaagaaat gtctgaaatc tgtttgtttt gctactgaaa
    26341 gtattgcata aggtgcaagt cttccagttt caattatttc ttcaatcagc ccttttgaag
    26401 gtacttcttt tgtaaaactt cttatggatc ttctagttct taatagcata tccaaaactt
    26461 cattaacatt gtttttatct aaattgttca tttttatcct ccactaatag tattcttaag
    26521 atgttctata atgcatccat ataaaggcga acttttcggt catattaaac atttccttca
    26581 aattaagtcg ctatttctat gaacacaatg ttgattaata atctatatgt atattataat
    26641 atttatattg cattatgcaa taaacaataa tatttaaact gttaagtaat aaacatttac
    26701 tataaaattg ttaattaaaa ttaaaaaaat ttatatggaa tgaggaaata attcatgaaa
    26761 aaagtatcta ttgatagaag aactatacgt actaaaaaaa tgattaggtc tgcattagca
    26821 gagttaatag atgagaaagg atttaacaat atatcagtta ctgatttaac tcaaagggca
    26881 gatataaata gaggaacctt ctatctgcat tatgttgata agtatgactt gttagagagg
    26941 attgaaaatg aaataataca ggaaattgaa gaggagacca aacatcttga ttcatccaac
    27001 atgatggaga tagatacctc aagtgaacct cttccattta tggtaaagct ttttgaatat
    27061 tttagaaaaa attcagtaat tattaaatct atcttgggtc ccaaaggtga ccctactttt
    27121 gaaagaaaaa taaaaaaatt tattgaaacc aatttgtttg agaaacaaaa actaataaat
    27181 tttaacatag atgaggcaat aatttcagaa gaatatttta tacaatatat actttcagca
    27241 gacttaggtg taattcaata ttggcttgaa aaaaatatga aagagtcccc tgaagaaatg
    27301 gcactgatac ttgctagaat gtcgttactc ggtccgttaa gagcagtggg aattcgtaaa
    27361 gattcataaa aatagtttta tatatattag actttgaaaa tatatgttta aaaaccattt
    27421 tttactacta ctgtttataa attaggggag atatcttaaa atattagtaa aaggagtgct
    27481 aagtgagtgt aataactatg agataagagg tttagttgat atagtcaaaa cttcaaaaga
    27541 atcaattaga aaagttaaaa tattgcaatt aaatttaaag gagtaatata tgaaaaatga
    27601 aaatataagt tatttggcgt taggattatg ttttggtgtt gttttgggtt cattatttaa
    27661 aaacttagct attggtttat cctttggatt agttattgga gtagtattag acagtaagaa
    27721 aaataaataa aacttcaata gtaacatata acaattatac tatttttgta taaaatcatt
    27781 ggaaatcaac aatagaaaat atttgttatt taaaaactaa aaatacagtt taaacgacct
    27841 aaaaatatat tatataatta ttataacaag ataagtgaga gtaaggttat gataatagag
    27901 taatattttt caattattac tctatttata tttatttagt tatactatag tttatattgt
    27961 tattttattt tcatatgcag tatagggaat ggattacctt gttcatcgaa ctcagatttt
    28021 ttaaagactt taaatcccat atgtttataa aagcctaatg cttgttgatt ttgctcattc
    28081 acatctacat aatttacatt taatacttct attgcatatt ctacaagtct ttttccaatt
    28141 ccatttccta tactataatt acttacaaat aacatttcaa ttttaaaatc atgtattccc
    28201 ataaaagctt ttataatacc tttttttgtc tcttacacat aaaaaattac taacatattt
    28261 agcacattct ttaacttgag gctttattga tattatattt tcttcactta aaaagtcatg
    28321 tgttgctctt actgatgatt cccgtgtatc tagtatttct ttcatatctt tattgttaat
    28381 aatatttaaa ttttcaattt ttatcattta ataattcctc ttaagtaagt atgttactta
    28441 atattatatg ttaaatatga tttaaaaagg ttataagaat atttttgtat taaatataat
    28501 caaataaata tatttatttg tcataaatta aatgtccact aaaatagaaa aaataagaag
    28561 aaataggtaa taaatgatag cttatagcaa aataaagatg taagtataac aatagatgag
    28621 attggaatta agtcactata cctaacagaa cattcttagg cgaatataga agacttaaaa
    28681 aagaaaagag aagtagacaa gaatttatta atgattacat ataaaataaa tgaattataa
    28741 ttttattaat gatataataa taattaacaa aaaataaaag aggaacgagt taatgtttga
    28801 tggcatacac tcattttcta accaataaaa caatagattc aattaaggtt actaaagtct
    28861 aatcaacaca taaacatata ggataaatac atctttttaa tatattaatt atgttagact
    28921 tacaaaaaat gtaaatacat agcttgttaa atcttgattg aaccttaagt aataagggat
    28981 aaaaaatgac ttgttcaaag gcaaagaata tactattaaa tgatataagt aacttagaga
    29041 taatatgtag taagtcaaaa gaactatata gcatcagtaa gtaaaaagcg taaaaactta
    29101 cataatgcac tataaaataa atgagttaga aaataaatat aatttatata ataaagtagt
    29161 acaaaaagga gtatttatgt ttggatatgt taaaataaac aaaatggatt taacatttag
    29221 agaatatgat tattacaaag cttactattg tggactatgt aaatacttaa aaagaaatca
    29281 tggagaaatc tctaggtttt ctttgaatta tgatataaca tttttaattg ttttattaac
    29341 agcagtatat aacccagagt ctataagtac agaggaagtg tgtatagtaa atccatttaa
    29401 aaagaaaaag gtaattacaa atgatattac agaatatgca gcaagtatga atattctttt
    29461 aacgtattat aaattggaag ataatttaat ggatgataaa cgcataaaag ataaattggc
    29521 atattatata tataaaaaca aattaaaatt agcatatgaa aagtatcctg aaaaagcaga
    29581 gtatataaaa caacaactaa atgaattaaa taagttagaa aaagataaaa atataaatat
    29641 agatgaagta tctagtatat ttggaaatat aatgggtgaa gtatttgtct ataaaaaaga
    29701 tgaaaatgag agaaacttaa gaatgatagg ttttaatata ggtaagtata tatatctact
    29761 agatgcctat gaagatttag atgaagattt taagaaagga agatataatc catttataga
    29821 gtatattgat aaaaatgatg agttaaaaga aaaagtaaaa aaaataatag taacatcatt
    29881 aggattttta gctaggggaa ttgataatct aaatctaaat aaaaatgttg gaataataga
    29941 aaatataatt tattctggtg tttatttaag atatattaat atattagaaa gtagaggtgg
    30001 aaagaatgta cagtaatact tataataatg aaaattttat gagagcaaga gcattcatag
    30061 aaaatagtga atttaaaaaa gcatatgatt ttttaaaaac tttgactgac aaatgtgcag
    30121 agtggtatta tttaactggc ttttctgcaa tgaacatagg atattatgaa gaaggtgaag
    30181 acttcttaaa aagagcaaaa tttatggagc ctgaaaatag tgaatatagt gatgcactta
    30241 gaagttatac acaatataga aatgattata gcaatagggc tgacaattat aatagaagaa
    30301 gacgtaatga tttagatgga tgttgttgct gttgctgtga tgattgctgt tgttgtctag
    30361 gtgacgattg ttgtgaaaat tgtgctaagc tttggtgttt agattcatgt tgtgaatgtt
    30421 ttggtggaga tttaataact tgttgttagg atgataaata tgagtataaa agttgcttat
    30481 ggaggagttt tattagcatt aaatgtgata ttgttgactc ttacaaatat tattcctgta
    30541 aatacattgt ttattatggg gttagcatca cttttagtat caattgtaat aatggaatgg
    30601 ggctttaaga gtggaatcgc tttttatata ggttctatag ttttaggatt tatagtgatg
    30661 gcaagtaagt ctcaatggat tgtatattca cttacctttg gaatatatgg tattgtaaaa
    30721 tatcttatag aaaaagatag gtctatatat attgagtatt ttatgaaatt agtatttgca
    30781 aatataatga tacttatatt gtatttttta ttaagaacaa tagtttatat tccaataaac
    30841 atatttatta taggttcatt tgagatagcc tttatagtat atgactatgt ttacagttct
    30901 tttataggtt attacaataa taggttaaga aagatgctat ttaaaaaata atagttgatt
    30961 tttatattta attttaatga aattgtagtt tggataagtg taaatgatat atctgaatat
    31021 acattaaagc ataaagctaa atttaatata ttcttagtgc aacatataaa agctttgatt
    31081 tttagtcaaa gcttttatat gttagatata aatattagat ataatataag tttttaaaat
    31141 agatagtata aattaaatat ccactatatt tacatctgta gatagagaat aaataatatc
    31201 ttttctttct actattatct caacattttt agaaatttcc tcatcaagtg atatttgaga
    31261 taaaagttcc tttgttggat ttattgctat tggatttcca actcttttta gcatatttat
    31321 atcaccatta gtatctccat aggcatacga gtttgataaa tctaaatcat actttacaac
    31381 aaaatcattt atagcattat tcttacttat agaatcccac attggaataa ctgtgcctgt
    31441 aaatatatta ttttcaaaga cataatcact tcctagaaaa tcggttacat tatatttttt
    31501 tgccattttt tcaacaagaa atccagggct accagatata aatataacta tgtgtccttg
    31561 atttagatgc cactcaatcc ttgaacgtgt gtatttataa actctatctg cttttaattt
    31621 aattacttgg tcacttgtaa agttgataca ggttttatct aatcctttta gtgaatcaac
    31681 ataaaggtca catatttcta atagataatc atcataatta ccttgtcttt tatcccaatc
    31741 cataaaagtg tctctagcat gtttatacca atttttttgg tcaattatat cgtatttaat
    31801 taatttttta aaatgttcaa ccataagaga gtctctataa agagtaccat ctatatcaaa
    31861 aaaagctgct atatttttca taagaccacc tcaattaatc atttccttat attatattat
    31921 aaattgtatc taagttgtta aaatatatgt ataataattt atatattaaa aaacaagtat
    31981 tttagagttt aaaggatgtg actatatgaa ttttaatttt gcaaaggcta caaattctag
    32041 acttatgggc agtttaggtc ttataataaa ttggatagat gatgaaaaca atcacttttg
    32101 tcaatatttt ttattagatg cagagggatt aggattagca gattatgtga gtttaaataa
    32161 cccaacacaa gaagaagctt atatggaaga agaaagattg atgggaggat ttggttcaga
    32221 tagagttgaa ttaacgaagg atgaatcttt atttttagta tcttattttg gtaataagaa
    32281 tctttattat gataagctat tgccaggaga taaatgtgag tatattgata ttataaaaaa
    32341 ctataaaaca gatttaatta tagaaaaatt atacaataaa atatgtaaaa gagttgatga
    32401 ggaagttgaa tttataaact atatgactat gagatttata gcatgggata gagaaagttt
    32461 aaaatatttt tctggaagtg acgagattgc aaatatgcat attactaata taaatggaac
    32521 attacttaaa aacgtggtaa gtgataaagg tcaaggcaga tatataagtg aagctttata
    32581 tgaagacaat gatggatatt atatatgtaa aattgctttt tgcataagta agtgctatga
    32641 aactggtttt agaattaatt ctctattagt gacagataaa gaacttatgt atgattttga
    32701 ggtatttgat gagatttcta aaagtgaatt tgtaagtgtt tacagtgtaa attcacctga
    32761 agaatttgca ggagtatttt atagagaaaa tccatttctg ttaaaaagta atatgaatga
    32821 aggtatattt tttactagat ttaattttaa taatgaccat gtaaaagaaa atgtatatat
    32881 aataaacaat gatatgaaag ctatatatta cttaatggga aataagtttt ttattggtac
    32941 ttataacgaa aatgattgca attatatcaa tgaaataagt cagtctaatt atagtgacta
    33001 tattaaattt gaagagaaat ttttctttga gcaaaatgca ttatatgact ttgcagaatc
    33061 aggttcatta gattttgatg attttttgga ataataaatt tagacattat ttaagcgaga
    33121 ttaactgaat ttaatctcgc ttaaatttat ttctataata ttgggggaat attatgagga
    33181 atacatttat tattgtggtc aattattaaa attttataca gtttattata aaaaatttat
    33241 acttttcttt atgttgtttg ccaatattag tctcaaatac gagtaccata ctattctgac
    33301 ccaccaacac aagaaagagt tttttaaatg aaaaagtcag ataaaattaa attttatctg
    33361 actttttcta tgtttatctt attagattat gttaatatct gttgaccgaa taagcgcata
    33421 ctcagtcaac tttctatacc tataataata ttgggggaat attatgagga atacatttat
    33481 tattatggtc aattattaat gttttataca acttgctcaa aaaaatatat attacatttc
    33541 ttcctcatca aattcttcaa tattagtagc ctgtggtata ccttgtatag acatattact
    33601 agaaccataa ggagtaggat atatattagg aggcatagta ctaggcataa ctacaggcat
    33661 accttgcatt gtattatgac atataactcc tggtatcatc ataagtacat taggaggcat
    33721 attttgatta catgagaagt taggaatacc catagtatta ttcatattat accccatatt
    33781 actgttcatg ttattaggca cattgttagg catacccatg ttactattca tattattagg
    33841 catattgtta ggcataccca tgttactatt catattatta ggcatattgt taggcatacc
    33901 catgttacta ttcatgttat taggcatatt gttaggcata cccatattac tattcatatt
    33961 gttagacata ttgttaggca tacccatgtt actattcatg ttattaggca tattgttagg
    34021 catacccata ttactattca tattgttaga catattatta ggcatacccg tattattatt
    34081 catgttatta ggcatattat ttgtagcaga tgtattgtaa ttcatatttc cggatgtatt
    34141 atttgtgtta ggcaagttgt ttggagaatt actactttta ggataatgat ttacattatt
    34201 aggatatagg ttatttatgt tctggttatt aggtattttt gtataaccat tagaaatttg
    34261 agggtcattt ctcaattcat cttggggaat ttcttcatta gtaatataat ctgcatagtt
    34321 cgctgggtct aagtaaggca tgtaactagt atctaaatcc ttttttttct tgttactcat
    34381 attaaacttg cacctcctag aaaactataa aaaagtttac aaaaacttat tctaataata
    34441 tatatattaa aaaagtttat aatatgtgca ttctaattat attaaattaa taaaaattat
    34501 tgttgcaaat ttaggaatga tagcatataa tgaacatatg aacagttatt catatattaa
    34561 aacaatggag gattaaagat gagagaagag ataaatgatt gtaattgtaa tatagtacat
    34621 gaagaaatag taacagaggc taaatcaact atgccagatg aagagatgtt atatgattta
    34681 gcagaattat ttaaagtatt tggggatact actagagtaa agatattata tgcattattt
    34741 gcaaatgaaa tgtgtgtatg tgatatagcg agtttattaa atatgactca ctctgcaata
    34801 tctcatcaac ttagagtact aaagcaggct agattagtaa agtttagaag agaaggaaaa
    34861 acagtgtatt actcattaga tgatagtcat ataagccaga tatttgattg tggattaaat
    34921 catattagag aaacttataa atgataaatg agaggagtga atatatatgg aagcaaataa
    34981 tttaattaaa aaagaattta tactaggagg attaaactgt gcacattgtg cagaggagat
    35041 taataataaa gtttctaaac tacaagaagt taaatcttcc aacttaaatt ttataaataa
    35101 aaaacttata gtaaatataa aggaaagctt taatgaggat actactatag aaaagataat
    35161 agatataata gattcaacag aaccaggtct tgatatacaa ataagttcta aagaaaatgc
    35221 atctagtaaa acatcaatta aaaaagagct tatattagga ggattaaact gtgcacattg
    35281 tgcagaggag attaataaca aggtttctaa attaaaagaa gttgaatctt ctaatttaaa
    35341 ctttgtaaat aaaaaactta cagtaaatat aagtaataac tttaaagagg atgatgttat
    35401 aaataaaata aaagaaataa taaattcaac agagccagga cttgatatac aagtagggtc
    35461 tactgacaaa gtaaaaggga gaactactga aaaatcagga gctgtaaatg atacaaataa
    35521 gaaagaatta gttccactta taattggagc acttgtatat atatttggta tatatcaaac
    35581 agctacaggt tatgaaagtc agtttagcaa tatagtattt atagttgcct atgtaatagt
    35641 aggtggcgat gtgcttttaa gagcaataag aaatatatca aaaggaagag tatttgatga
    35701 gaactttttg atggctctag caacagtagg agcattaaca ataggggagt catcagaagc
    35761 agtaggggtt atgcttttct ataaagtagg agagtattta caaggtgttg cagttggtaa
    35821 atccagaaag tctataactt cacttatgca aataagacca gattatgcaa atctaaaatt
    35881 taattctgaa gttaaggtag tgtcaccaga agaagtaaat gtaggggata taatagtagt
    35941 aaaacctggt gaaaaagtac cattagatgg tgtagtagtg gatggagttt ctatgctaga
    36001 tacttctgca ctcacaggag agtcagtgct gagagaagtt gaaaaaggag atgaaatctt
    36061 atcaggtgtt ataaataaaa atgctttatt gagtattgaa gttacaaaga gttttggaga
    36121 atcaacagtt tctaagattc ttgaccttgt tgaaaattct agcattaaaa aatcaaagac
    36181 agaaaacttt atatctaagt tttcaagata ctatactcca atagtagtaa tagctgcttt
    36241 attaatagca tttgtgccac cacttgtaat ctcaggtgag gtatttagtg attggttata
    36301 tagaggattg atattcttag tcgtttcttg cccttgtgca ttagttttat caataccact
    36361 tagtttcttt agtggaattg ggtttgcatc taaaaatggt atccttatta aaggaagtaa
    36421 ctatttagaa gctttaagaa gtgtagatac agtagtattt gataaaacag gaacacttac
    36481 taaaggtgtg ttcaatgtaa ctaagttaaa tcctgaaggt atatcagaag aagaattatt
    36541 agaatatgca gctattgcgg aggtaaattc aaatcatcca atagcaaagt ctatattaag
    36601 ttactataat aaaaaaattg atttagatac aatagacagt tatgaggaaa tagcagctta
    36661 tggaattaga gtaaagcata atggcaattt tatattagca ggtaatgaga aacttatgaa
    36721 aaaagaaaat atatcttatt catcagctaa agaagtaggc acagtagtct atattgctgt
    36781 tgataaagta tatagaggtt atatagttat atctgatgaa gtaaaagaag atagtaaaaa
    36841 tgccataaga agcttaaaag caataggggt aaaagaagtt gtaatgttaa ctggagataa
    36901 tgagaaagta gcaaaaaata ttgctcaaga attagaattg gatacagttt actcaaattt
    36961 acttcctaat gaaaaggtag atagattaga agatttatat gaaggaagaa ctgaaaaaga
    37021 gaaaatagca tttgtaggtg atggaataaa tgatgctcca gtattagctc gtgctgatgt
    37081 tggtatagct atgggaggct taggttcaga tgctgctatt gaagctgcag atgtagtgct
    37141 tatgacagat gagcctagca agatatctaa agcaattgaa atagcaaaca agactaataa
    37201 gatagtgtgg caaaacataa tatttgctct aggagtaaaa attatagtta tgatactagg
    37261 tgctggagga gtagctacta tgtgggaagc tatatttgct gatgtaggtg tggcacttat
    37321 agcagtagta aatgctatga gagctatgag ataagggata aatgtaaatc catactattt
    37381 aaaaaactta taattatata ttgaagtatg taataatgaa aggtatcaat aagtttcttt
    37441 taactttatt ggtacctttt tattggagcg ctttgcatat ataaatttat attcattatc
    37501 cattgtgttt atttaatgta tttatttagt gtagagcttt tatattgtaa ttttaaaatt
    37561 tagacataga aggtttatta ataagtctat attaagttta aattagattc ataaaaatta
    37621 taatacttat gattgtatat atattatatt aatagttata atttttacaa caaaatgtgt
    37681 gtgttaaaat actctaaacc aacagtatga ctgtcattca ataaattatg taatataaga
    37741 aagtaaatat ttttaaaaaa taatttaaaa aagtattgac gaaatatttt taaggtgata
    37801 tagt
//
LOCUS       DAELXX010000025        37180 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.025, whole genome shotgun sequence.
ACCESSION   DAELXX010000025 DAELXX010000000
VERSION     DAELXX010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37180
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.025"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            196..762
                     /locus_tag="KPH29_001244"
     CDS             196..762
                     /locus_tag="KPH29_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1466867.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            complement(981..1967)
                     /locus_tag="KPH29_001245"
     CDS             complement(981..1967)
                     /locus_tag="KPH29_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase"
                     /protein_id="HBG1466868.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGARAYVSIAGGIDVPLAMGSKSTYTRSKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLNSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISDGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(1997..2692)
                     /gene="pxpB"
                     /locus_tag="KPH29_001246"
     CDS             complement(1997..2692)
                     /gene="pxpB"
                     /locus_tag="KPH29_001246"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBG1466869.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            complement(2712..3932)
                     /locus_tag="KPH29_001247"
     CDS             complement(2712..3932)
                     /locus_tag="KPH29_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HBG1466870.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVDTMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            complement(3961..4752)
                     /locus_tag="KPH29_001248"
     CDS             complement(3961..4752)
                     /locus_tag="KPH29_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HBG1466871.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRSVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELEGIEVCPLENIEVCSSENIV"
     gene            5341..5970
                     /locus_tag="KPH29_001249"
     CDS             5341..5970
                     /locus_tag="KPH29_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG1466872.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRAVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            complement(6084..7598)
                     /gene="glpK"
                     /locus_tag="KPH29_001250"
     CDS             complement(6084..7598)
                     /gene="glpK"
                     /locus_tag="KPH29_001250"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBG1466873.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            7841..8383
                     /locus_tag="KPH29_001251"
     CDS             7841..8383
                     /locus_tag="KPH29_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG1466874.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            8397..10889
                     /locus_tag="KPH29_001252"
     CDS             8397..10889
                     /locus_tag="KPH29_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG1466875.1"
                     /translation="MNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPARTII
                     QNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLICGL
                     SSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIANIL
                     GSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGTLML
                     ICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILSFEY
                     FLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIAAPL
                     SGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFTMGT
                     PLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDLVGK
                     PSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKSLLN
                     KVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGENKL
                     NSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLEKQDEAIKELTT
                     AYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVNEQK
                     ESLNAQIQQLKKTRDDLNTKLQKNKTQKNELSKKLRSMESQKKELQSKLNNSINQKKD
                     MEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTLQST
                     LNSGFKQMYITVFCVNLLAFIILLFYKENKTR"
     gene            11310..11708
                     /locus_tag="KPH29_001253"
     CDS             11310..11708
                     /locus_tag="KPH29_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1466876.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMMILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
     gene            complement(11925..12134)
                     /locus_tag="KPH29_001254"
     CDS             complement(11925..12134)
                     /locus_tag="KPH29_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466877.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(12166..12375)
                     /locus_tag="KPH29_001255"
     CDS             complement(12166..12375)
                     /locus_tag="KPH29_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1466878.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            12674..13093
                     /locus_tag="KPH29_001256"
     CDS             12674..13093
                     /locus_tag="KPH29_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1466879.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLGKIEYNKNKDEKIDK"
     gene            13321..13776
                     /locus_tag="KPH29_001257"
     CDS             13321..13776
                     /locus_tag="KPH29_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466880.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
     gene            complement(14001..14261)
                     /locus_tag="KPH29_001258"
     CDS             complement(14001..14261)
                     /locus_tag="KPH29_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466881.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTDIKGMLQNGV"
     gene            complement(14524..14877)
                     /locus_tag="KPH29_001259"
     CDS             complement(14524..14877)
                     /locus_tag="KPH29_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466882.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQYKDISDISIGQAKQDDDITNNFI
                     ANAEIYEMLLSQSSVNEASNISTFSVRKSGGESGMVEVYVALILRGRKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            complement(14894..20071)
                     /locus_tag="KPH29_001260"
     CDS             complement(14894..20071)
                     /locus_tag="KPH29_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome condensation regulator"
                     /protein_id="HBG1466883.1"
                     /translation="MKQNKLLQRGAYFNDKNILIDDFDKRYNDYDFVEFFTGISNSTF
                     GLKSDGNLYACGDNTGFQLGLGKDSSERRMFSKVKIDNVKYVSCGSKHSVAVTKDGFA
                     YGAGTSNVGQLGVIESTVYYEFTKLPIDDVKTVACGYDFTFVLKNDGTLYSAGLNSSG
                     QLGLGDTNNRATFTKVNIDSVKDVVTYNQSVFIIKMDGTAHACGLNSNGQLGINSTLN
                     KSVFNKIEGMDNVKQIACGSSHTILIKNDGTMYTTGYNGVGQLGTGNNNNSIVFTLSS
                     INNVKYASCGNNHTMILKYDNTLFSTGQNNYGQLANANKDVASRNTFAKVNVENIKDI
                     KCGSQFNFLINGSKEIFVSGCNLAGQLGSFFHTTFLYEFSNVQSSNLDNYSGLLVNDD
                     YLYVTKDNSEFLNVKLSDNFQDYKKIELTDSNMFIVMNDGTLYACGLNNYGQLGLGDT
                     VNRSVMTKVDIDNVLDIKGNGNSTFVLKNNGTLYSCGLNSNGQLGLRDEVNRNIFTKI
                     EIENVKDFCVGSNYVIALNHSKEVYGWGNNPYNNIEKTSNYPYKQGISNIEKIAAYDY
                     SVYMINSEGKLYVSGYNYNYQLGKGNNSNQSKALVSQCRTNSTSSTSNGLRTLPKITN
                     VFPFYDGCAIIDEGGYVYLTGYHGYLRTLNSSPSISDYSRYGTFIEATNSNHNTYFIQ
                     ETDFSGIEKVIGMSNNILFFKKGSSYITGYPKTFGSTITGHRSYTSINSESSNLGSNF
                     IIYHSNSKLYGKGIANSGQFGNSTNIDGTSNYDTGLKDIKDIIVKGNTVVVVDKNNNI
                     YVTGMNQNNKLGIGEYNNEPVKKFTNITEQSNSFIFMDDIKEITTSRNTMFIVKNDGT
                     AYATGNNSSGQLGLGDTINRNKFTQINLDNIKKISTSIDGNTTFAIRNDGTLYSTGLN
                     TKGQLGLGDIVNRNTFTKVNIQNVRDVVLGTTHSHAIKDDNTLYSCGENTHGQLGLGS
                     ESNHPDVLTFTVNNITNVRDVYCSDTTTFIVKDTNIAYCCGYNNNSQLGMGNTTDQYS
                     FIKCMENVKEVIPNEINTYIITIYNTAYSTGLNTDYCLGLNSNSNQSSFSEIPISNVV
                     KVAPNRNNAVLLLTSEGDVYTAGKCSNGSGTGSETPEKIKKIASKAKDIGMNYRCGHY
                     VSDNGDLYGTGFNNNGQLGVGDVTKRDTFIKTNTRVKKILPLEYANIAIKDTNDIYIC
                     GLNNYGQLGVGNRYDSRNNDNRIFNYKHMNFVMGDLTSIKNRHNFILLNNKIVIPTTK
                     DIDYGLVLGNLYKGDLYTELPYEDIKEVSISKTHIIILLNDGTMYGCGTNYHGELLQD
                     LSINQVDEFVQINVSDVKHVSCGDNFTYFIKSDDSLWSIGKNSEYQLGIGHNNPVTEL
                     QRITTISSCKEVHCGKNYTLVVTTGNELFVQGYNDKGALGLGSDSENTIIKFFTKALT
                     DIREIKSYGSDHILVLKNDNSVWVTGKNRDVYKIEQPVEFLKEFTIVPISEDVNTVKD
                     VLATDNTLYIISEVGTTNAAIEITEKSISSIKIKIQDPNKDISRIEMLINGESVKSVS
                     DLTTEKISFEVPPDKIKIGENKILFRAYCKGDDLYASLFIFKESTGNSIIKDSYVMIG
                     NRMYKVVNTTSNEQDITITLDRGLEEDLNLGDPIYQLINKTKVQVKINKSDLFKDMKL
                     VEIKKSDSSYQEIYELEEANIKSAQPKIIVEKGDKWTAIKRPSMIFRYDAENNEPQA"
     gene            complement(20093..21079)
                     /locus_tag="KPH29_001261"
     CDS             complement(20093..21079)
                     /locus_tag="KPH29_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG1466884.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKFD
                     KNIKEIKVKIGDTDILTTDSKDLSGAINEVVKKIENISFDDVISGQIQTDISVLKNSY
                     NKLSEKVLDILIYLELESEVTVDEAGYWYDTLANGNNIVAIEGLKLDLNRKCITGEIG
                     NVIFRDVVLPFSANRVRYIHDMDNNFVETKSSNTYLKEQKDITLSKYSYEIR"
     gene            complement(21080..21778)
                     /locus_tag="KPH29_001262"
     CDS             complement(21080..21778)
                     /locus_tag="KPH29_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HBG1466885.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSEMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMLSREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(21775..22827)
                     /locus_tag="KPH29_001263"
     CDS             complement(21775..22827)
                     /locus_tag="KPH29_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBG1466886.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKIHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTII
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGENNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEISISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTTGVHDLSNLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            complement(22820..23248)
                     /locus_tag="KPH29_001264"
     CDS             complement(22820..23248)
                     /locus_tag="KPH29_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBG1466887.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDVLSANVKVSTIYGEVEINV"
     gene            complement(23248..23574)
                     /locus_tag="KPH29_001265"
     CDS             complement(23248..23574)
                     /locus_tag="KPH29_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HBG1466888.1"
                     /translation="MANPINEFIGIIREEGKYHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMIDRQLETFDTETNQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            complement(23590..25119)
                     /locus_tag="KPH29_001266"
     CDS             complement(23590..25119)
                     /locus_tag="KPH29_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG1466889.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGTIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVTLSVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYAGGKEFTAEFTA
                     YCPRKEEGGDTDCRKKKLDPSKKTCAAPMVGKYEQTYYTKEFLNKHPLLNYGDEIQVI
                     TGVSGRDGVYKVNDVGPAITIEKNGTYHIDILFGNVEEASKFGRRKGKIIIGGYSGNV
                     SDKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKGK
                     KVEQKNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQKGDVVKISKLSSRRDFNTARRV
                     L"
     gene            complement(25401..25823)
                     /locus_tag="KPH29_001267"
     CDS             complement(25401..25823)
                     /locus_tag="KPH29_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466890.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEVATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(25823..28276)
                     /locus_tag="KPH29_001268"
     CDS             complement(25823..28276)
                     /locus_tag="KPH29_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466891.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASSRVNSLNSSLRKVQTTASSVSSTMAKLSSSINAVSGVIGSLNGSI
                     MRLAITIAMIIDYFNKLIQKKNEFNSNIMIILIFKAKSDEVEKTKNKLLGNLKKIGGK
                     IWNIVIKAKDMTKRVISSILGKLKRVEKRPYQGSINLKDMVSSAMARILPKLMLFKNT
                     FWSGVIAIKDMASSIISKVFPKLRLFAGKVWSGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKTSQKDVDKYYDSLVNMANDTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSEKMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIEPILGAFFNILDKVCKIV
                     KDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAGTKAGDSGKAYSSKKGNN
                     AFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNINIAKLADTIVIREEADIE
                     KITSKLVASIQLAQLGGVL"
     gene            complement(28276..28443)
                     /locus_tag="KPH29_001269"
     CDS             complement(28276..28443)
                     /locus_tag="KPH29_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466892.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGM"
     gene            complement(28470..28916)
                     /locus_tag="KPH29_001270"
     CDS             complement(28470..28916)
                     /locus_tag="KPH29_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG1466893.1"
                     /translation="MNENGLSKNINIVDLLLNADTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(29030..29458)
                     /locus_tag="KPH29_001271"
     CDS             complement(29030..29458)
                     /locus_tag="KPH29_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBG1466894.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYMGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(29472..30536)
                     /locus_tag="KPH29_001272"
     CDS             complement(29472..30536)
                     /locus_tag="KPH29_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBG1466895.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESRVNKGELILIKEAGAIR
                     IARGVNSLTELTEEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(30541..30984)
                     /locus_tag="KPH29_001273"
     CDS             complement(30541..30984)
                     /locus_tag="KPH29_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466896.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPIMLNTNNKDIVISIKYLPKPQSKSIRMYEISDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTITLNYLDSVYEEDVVYENMEEINLNLGE"
     gene            complement(31233..31673)
                     /locus_tag="KPH29_001274"
     CDS             complement(31233..31673)
                     /locus_tag="KPH29_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466897.1"
                     /translation="MENKKDILFKETDERLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(31778..31975)
                     /locus_tag="KPH29_001275"
     CDS             complement(31778..31975)
                     /locus_tag="KPH29_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1466898.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQL"
     gene            32144..32464
                     /locus_tag="KPH29_001276"
     CDS             32144..32464
                     /locus_tag="KPH29_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG1466899.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            32546..33052
                     /locus_tag="KPH29_001277"
     CDS             32546..33052
                     /locus_tag="KPH29_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466900.1"
                     /translation="MNNLDKLFELASQEEIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
     gene            33753..34508
                     /locus_tag="KPH29_001278"
     CDS             33753..34508
                     /locus_tag="KPH29_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG1466901.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
     gene            complement(34651..35079)
                     /locus_tag="KPH29_001279"
     CDS             complement(34651..35079)
                     /locus_tag="KPH29_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBG1466902.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(35500..35700)
                     /locus_tag="KPH29_001280"
     CDS             complement(35500..35700)
                     /locus_tag="KPH29_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG1466903.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            complement(35883..37052)
                     /locus_tag="KPH29_001281"
     CDS             complement(35883..37052)
                     /locus_tag="KPH29_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HBG1466904.1"
                     /translation="MGGVALKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNM
                     EEKVMESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYE
                     NEVKKMKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQK
                     DKENAFRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSEDIRNYIGQQDGV
                     ELQLTDEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGR
                     FKKNSIWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFV
                     GYEKKGRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
BASE COUNT        12125 a         6329 c         4177 g        14549 t
ORIGIN      
        1 attaaatttt tttattatta ttttctttat attaatttaa caataattca ttttcataga
       61 cttttatttg gatttaatta aattttaatt gtcaaaatct actatgtatt cttattgcca
      121 ctacttctat gttgtgctat aattactttg ttttaagcga atattttata ttaattttat
      181 tatttggagg ttcttatgct aggtgaaaag atgagaaata taagaaaaag caagaaaaaa
      241 acattaagtg atgtttccaa attgacagat ttatctataa gttatatctc tcaaatagaa
      301 agagatgcta ttgaaccatc cttgtcttca ttgagaaaaa tcgctgaagt tttagacact
      361 cctttatata tgtttatgga tgacaataat actgatgatt tagtaattag aaaagaagat
      421 agagttatga tgaaatttcc aaagagtgaa atgttttacg agatagtatc tccaatgcct
      481 acaactgaat ttacaccttc aattttattt gtagagtttg aacttaaacc agaaagtgag
      541 gatgctaaag attatatcta tcatgcatca gaagaaatta tggtggtaac gagtggtgtg
      601 gttgatattt gtatgggtga aaaggtagtt agattaaatc caggtgactc aacatttata
      661 aaagctaatg taccacataa aatcatcaat ccaagtaaag atacaatagc tcgtggatat
      721 ggagttatat cacctccaat ttggccaata aaatctaaat aaactcaaca taacacacct
      781 actttttgta gataatataa taaatttaaa tattgctttt attttaaaat tgaagtattt
      841 atttaaattt aaaatattta ctcaaactaa aaaaatagct atttgactaa tctaaaccat
      901 cttaattatg gtaaatagta aatagctatt tgtaagttat attataaatt ataataaaaa
      961 ttgtatttgt taaaattaaa ttaaactgct atatcattta atacttttgc ctttacaact
     1021 cttatatttt tcatactctt gtagatatct tgaatcttat cttcttgctc tctcaatagt
     1081 ctatgtgctt catatatatc tacaagttca aattttacta catcatttgg ctttgcttgt
     1141 gctaacttat acaaatctac acttataacg ttccctatct tagtatagcc accactagtc
     1201 tgtctatctg ataacattat aatcggtttt ccatgaccag gtacttgtat agctccataa
     1261 gaaataccat cagatataat atctgctcca ttaaggtgct ttatactctc tccatctagt
     1321 ctacaaccca ttctatctat ttcattcgta actttgtatt tatttgataa aaatttctct
     1381 acttcaccgt tagaaaaagc atcaaattga ggacctttta caattcttag tacgatttct
     1441 gaactataag ttggaatata ttttgaattt aattttttat ttattgtaaa atctttttcc
     1501 tgaataaaag tatttatata atctcctttt ttaagtgctc taccttcaaa tccgccaatt
     1561 ttagatcttg tataagttga cttacttccc attgcaagtg gaacatctat tccaccagct
     1621 attgacacat aggctctagc acctgatttc acacctctaa atgataatac atcacctgga
     1681 tttatttgat atgacctcca gttttgaatt tcttttccat ttattttagc ccctaaatct
     1741 cccccagtta ctgctatttg cgtatattca tcaaaggtaa ttgtaggacc taacattaaa
     1801 acttctaaag aagcctcatc ttcagaattt cctactaata tatttcctac tctatgtgca
     1861 tactcatcca ttgctccaca tccagataca ccatattgac catagccaac tctacctctg
     1921 tcttgtatta atgtttggaa acctcctaat tcaactttga atcccatata taccactccc
     1981 tgtactataa cgatatttaa atacttaagt tatttttttc gatttctata aattcatcta
     2041 atgttattgg aacaaatttt ataaagtctc cagctctaag caaaatctgt tcttctctat
     2101 tttcatcata taattttaaa ggtgtcctac caattatttg ccatccacca ggactatcaa
     2161 taggatatac accagtctgg gaacctgcta ttccaacact cccaccataa attttagtcc
     2221 ttggcacttc taatctaggt gtagctattc tttcatccat accacctaga tatggaaatc
     2281 caggagtaaa ccctagcata tatatcaagt actctctgct tgtgtgtatt tttataactt
     2341 catctacagt gagattattg tagcttgcaa cgtttttaat atctatacca aaaacctcat
     2401 cataacatac tggaatctca tgtattttag gttttggtaa tactatactt tccaaattat
     2461 cttctatttt ttttaaattc tcaatcaaac tatcaaaatc tatttctaat ggattataac
     2521 ttatcataag tgacctatat gttggtacca tttctgttac aataccttta agattcgata
     2581 tttctattgc tctcataaat gaaattactt ttttgtttat atcttctgat atctcatttc
     2641 caaattcagc aaccactgcc ttatcccctg atatcaagta tctagtttcc ataaatgtca
     2701 cctcttctaa attaagcaaa taaagcacct aggctgctaa gagaagttat tcctgtatat
     2761 gcagatatta aaactactat taatccaaat attaaaagcc atgtaggatg cttgtaatct
     2821 ccaacaatat cttttctttt agatgctata agcataattc ctaatgtgat tggaagtatc
     2881 aatccattta aagctcctgc taaaattaat aaagtagctg gttttcctat aaatgccatt
     2941 ataagtgttg atattgctat aaatgctatt ataaaatatt tttcatattt atctataaaa
     3001 ggatttagtg tctttaagaa agaaactgat gtataagcag ctcctataac tgatgtaatt
     3061 gcagcagacc ataatacaag tccaaagaat ttatatccta ttgctcctgc actaaattta
     3121 aaagctgaag cagctggatt ttctgggtca agttgaagac cttttgaaac cactgccagt
     3181 attgctaaaa ataaaagaac tctcatcatt gtgtcaacta atatacccaa aagagaagat
     3241 ttagttattt cttttatatt ttcttctcct gttattccac cgtctattaa tctatgtcct
     3301 ccagcaaaag ttatataacc tccaacactt cctccaagta gagttattat tgggaatatc
     3361 aatccttttg gattttctgg ttttaccatt cttgatactg cctctccaac aggaggatga
     3421 tttgatattg ctacatatcc aactataatt atcattccta atgctaaaaa tttagttatc
     3481 ttatcgacta gagaatttga attttttgac ataaatacaa atatagctat taatccactt
     3541 aatacagttc ctaatgtcat attcatatta aacattacat tcataccaag tgcagctcca
     3601 ccaacatttc ctatattaaa agctaatcct ccaagagcta ctaaaaaagc tactaaataa
     3661 cctaatcccg gtattatttt atttgctata tcctgacctc taagtcctga tacaccaatt
     3721 actctccata catttacttg agcccctata aaaaggattg ttgtaattaa aattacaaat
     3781 gaaaaatttg gtccaaaatc ttgggtaaat tgtgcagtct gagttaaaaa ccctggtcca
     3841 attgctgatg ttgccattat aaatgcagcc cctataagtg ctccgacatc ttttttattt
     3901 ttagttttat ctgtaatatt ttttgtactc atattttatc tccccctcat gttatcagtc
     3961 ttatactatg ttctctgacg aacaaacttc tatgttttct aatggacaaa cttctatacc
     4021 ttctaattca aaacgctttc ttatttcttt gacaaactct attgcttttg gattgtcccc
     4081 atgtacacat atagaatctg ctttgatatg tataacttca cctgttaaag tttctacagt
     4141 tccttctttt accatcctaa caaccctatc tattgcatgt tttgtatcat gtataactgc
     4201 tccttctaca cttcttggaa ccaaaaaacc attattatca taagctctat ctgcaaatac
     4261 ttcatttgca aacctaagtc caatttcttt agcagaattt atcatttcac tattatatag
     4321 ccccaacagt ataatatctt tatcgacttc atatatagct tcacaaatcc ccatagctaa
     4381 ctctttatct ttagctgcca tattgtataa tgctccatgt ggttttacat gttgtatatt
     4441 acatccattt gaacttgcaa atgccattaa agcacccaat tgatattttg tataatcttt
     4501 agcttcctgc tttgttattt tcatctcacg tcttccaaat cctattaaat ctaggaaacc
     4561 tggatgtgcg cctattttca ctccattttt tttagctagc tgtactgttt tttccatatg
     4621 actagggtca cctgcatgaa acccacaagc tatattagct gatgaaatat attttaaaac
     4681 ttcttcatct aaaccaatct tatacgttcc aaaactttct cctaaatcac tatttaaatc
     4741 tactttatac aatttatgac cccctccttg ctaacatttt gatagaataa gtagttttaa
     4801 tttttaaatt ttgtttattt tatatattgc atgaaatatt ttatcacttg tattcaattt
     4861 tatttttgca actattctat tgcatttaat taatataatt ttaccattta aggaatattt
     4921 ttgcaattat tttaattgaa cattcatttg tatttctttt tcaaaaaatc tttttaccat
     4981 tttttagtta ataattaatt attttcactt aaaataaggg ccttaacctt catttttaaa
     5041 gtatttttca tttttaatca ctttatatgc aagacagaat catttttctt catttatttg
     5101 tttctttcat ctatataatt atcattcttt gtaagtaaaa tatctttata tttttataat
     5161 taattcttta tatagtttta agtataataa aatcatgtat actaaatgca tgttaaaata
     5221 ttattttaaa acatgcacaa tatttgtaca tattgtagac atataaacct ataaattata
     5281 caatagtatt gttaagatta tatatttttt taacataaat tttgtattgg gggaaaaaca
     5341 atggataaaa actatacgat gccaatatac caaaaaatag cgttggatat tgcaaataaa
     5401 atatacacgg gtgaaataca agaggattca gtactatttg gacgctctgt tttggctggt
     5461 aaatacaatg tatctccaga aacgataaga agagctgtca aaattttaga ggacattggc
     5521 gttgttaaaa gcataaaggg taaaggtgtt attgtactat ctccagacaa agcttcttct
     5581 tttataaaaa aatatagaga tattacaaat atatcatcat ataaatcaac actttataat
     5641 ttaatagata ctaaatctaa tttagaaaat gaaatattag atactataaa taaaatttta
     5701 gactattcca acagacttga aataatcaat cctttagtgc ccgttcagtt caccataaat
     5761 tctaattgca aatatattgg tcaaacagct gcacaaacta aattttggca aaatacaggt
     5821 gctacaatag ttgcaattaa aagaggtgaa gaacttataa tatctcctgg accatatatt
     5881 gaatttttag aaggagacat tctcttagta gttggtgacc aacacattta caattctata
     5941 ccaatgtttt tatatgaaaa cgaaaaataa attatataat ttagtatgat tatataaaaa
     6001 tgcaaaatta acaacaaaaa aagagcttca tattttacat aaagctcttt ttaaattcaa
     6061 ataaatttaa ctttgattaa tttttaaata tccaattcat cttctttttc ccagcttaaa
     6121 gctctcgata cagctttttt ccatccttta tataatttac atctcttctc ttcactcata
     6181 tttggattaa actctttttc tactgaccac tttgaagtaa tttcatttct tccaatatag
     6241 aatccaacag acaaccctgc taaataagca gctcctaaag ctgtagtttc tactattttg
     6301 ggtctatcta tatctacatt taatatatct gattgaaatt gcattaaaaa attattatta
     6361 cttgctccac catcaacttt taatgatttt agttttaatt ttgaatcgtt ttgcattgct
     6421 tctaaaacat cctttgtctg ataagcaatt gattccaatg tggctcttac tatatgttct
     6481 ttttttgctc ctcttgtaag tcctaaaatt gaacccctag catacatatc ccaatatgga
     6541 gctccaagac ctgtaaatgc tggcactaca tatacaccat tagtatcctc taccctattt
     6601 gcatacattt cactatcttt tgcagattca attatcttta attcatctct aagccattga
     6661 acagatgctc ctccaataaa tatacttcct tctaatgcat atgttacttt tccatcaact
     6721 ccccaagcaa ttgttgtaag taacccatgc ttagatttaa ctatgttact gcctgtattc
     6781 ataagtaaga aacaacccgt tccataagta ttttttgcag taccttcttc aacacaattt
     6841 tgaccaaata atgctgcttg ttggtctcca gcacatcctg caataggtat tttagctcct
     6901 gataacatgc gctcatctgt atgaccataa acatagctag aaggttttac taatggcaac
     6961 atgcttattg gtatgtccaa ttctcgtaat atatcctcat cccattctaa atcatttata
     7021 ttgtacatca ttgttctaga tgcatttgag taatctgtta catgtacttt cccacgagtt
     7081 aaattccata ttaaccatgt atctatagtt ccaaataata actctccttg ttctgctttt
     7141 tccctagctc cttctacatt atctaatatc cattttactt ttgttgctga aaagtatgca
     7201 tctattaaaa gacctgtttt ttcttttatt gattcttcca atcctttatt tttaagttca
     7261 tcacatattt ctgatgttct cctacactgc catactattg cgttataaat aggtttacca
     7321 gtatacctac tccaaacaac cgtagtctct ctttgattag ttatccctat tgaagcaatt
     7381 tcttctggtc ttatacctgc tgtttctata acttctctca ttacaccact ttgtgacccc
     7441 catatttcca tagggtcatg ctctacccat ccagcttttg gataaatttg attgaactct
     7501 ttttgtgcaa ttttcactat ttcgcctttt ttattaaata gtatagctct tgaactggtt
     7561 gttccttggt ctaatgccat tacatacttc ttttccatca ccatattctc cttatcatat
     7621 aatatttaat ttattaaaaa gaaaaattta atatcacata tttcaataaa taatcatact
     7681 taagtacaaa tttaatataa tagatattta taaaaagaca cttattttct atcatatcta
     7741 tataataata ttatacatta tagcttacat attgattaat aaattttaaa ggagtataat
     7801 aaacagtgtt cataataatt ttaataaaag aggtggtaat atgccaaaga ttttagaaaa
     7861 tgtaaaagaa gatatattaa aagtatctcg ggatatgatt ttagaagaag attactcgaa
     7921 tattagcata aggaaaatag ctcaaaggtg tggtataagt gcaggtacag tatataatta
     7981 ctttaattca aaacaagaaa taatagaata tatcacaaaa tctgaatggg atttattgat
     8041 aaggcgaatt gaatactcca ataaaaacac tgaagattac ataaaaaaac taaacattat
     8101 ttttactgaa ataagaaatt ttataaacaa agttcataat atccaataca atgacttttt
     8161 aaacactttt gaaacagaga gattttttga aatgaaaaaa cacaaagacg actttcatga
     8221 acagctttca aataaagtat atgaagcttt agaaaaagaa agttttttta ataatgacaa
     8281 attagtatgc aatatcataa taaaaatgtt tttttcatat tcaacatatc ttcatataga
     8341 atttgaagat ttaaaaccct atattgaaaa attaatacat taaggtggtg tttaccttga
     8401 atcataatac tagtattaag aacaaggaca gtaatccaaa aatattttta gtacttacac
     8461 tttatctatt aggtatattt atgggagcta ttgatactgg aatagtatca ccagcaagaa
     8521 cgattataca aaatagcctt ggagtaaatg aaaaaacggg aatttggatg attaccatat
     8581 acacattaac atacgcttct gtaattccta tctctggaaa attagcagat aaaataggaa
     8641 gaaaatatgt gtatctagtt agtatatttc tatttggtag tggttcatta atatgtggat
     8701 tatcatcact attttccaat ttctatattt tattaattgg tcgtgttatt caagcaattg
     8761 gtggtggtgg aatcatgcca attgctacag cagaatttgg aactaccttc ccagaaaata
     8821 aaagaggcat ggctttaggt ttagttgggg caacatatgg tattgccaat attttaggtt
     8881 caagcattgg tagcacaatt ctaagtatct ttggaactca aaattggaag tggctatttt
     8941 ttgtaaacct tccaatttgt ttaatcatac ttattggtgg agttttctgt attaaaaaca
     9001 ataagtctga atctaaagaa aaaatagata agcttggtac attgatgttg atatgtataa
     9061 tcgtatcact attatatggt cttatgaata tagacttttt taattttaaa aattctatac
     9121 aagatatatc agtttatcca tacttattga catttattgt acttatacct attttcatat
     9181 ttattgaaaa tagagctaaa gaccctattt taagctttga atatttttta aatccgagaa
     9241 tattgatagt acttatttta tctttaattg ttggtattgg aatgatgggt atggtattcg
     9301 ttccacagta tgctgaaaat gcattaaaga taaatgctgg ttctggtgga tattttgtag
     9361 ctatattggg attatttgca ggtattgctg ctcctttaag cggaagactt attgacaagt
     9421 ttggggcaaa gaaaatacta ttactaggat tttctgtatc tatgataggc tctctttacc
     9481 tagttttaat tgctctaaaa accaatactg tatttagtgt atgtgtaagt ttaatattta
     9541 tgggattagg aatgggcttt actatgggaa ctcctctaaa ctatatgatg ttatcaaata
     9601 caagattaga agaatctaat tcagcacttg caacattatc cttgatacgc tcaattggta
     9661 cttcaatttc tccagctata atgattggat ttatagcaca tgcaggatta tctgtgcaag
     9721 ataatattat ggacttagtt ggaaagccct caactcctaa aatagttcaa cttgaagaac
     9781 ttgaaaacat gatagatgac ttaaaatcag accctgaaat ggcaaaaaat ttaaaaaatg
     9841 tctctatccc aaatatgaat gatacttcaa atataaatat ggacatgaca ggtggcaaac
     9901 ttccaaaatc cttacttaat aaagttcagt cagcagatac aaccaacatt accaatataa
     9961 caaaagaaat atcaacaaca atgtttgatg aaaaagttcc atctataatt actaaaatac
    10021 agaaaaatgt acaaaaagga atagatggaa cacaaagtgg aataaacggt attgaagatg
    10081 gtgaaaataa actaaatagt ggtattaaag gagttcaaac tggtattgac aatatggtaa
    10141 aagctagagc tggtattaat caaggaatag atggtgtaaa aaaaggtatt gctggtgttg
    10201 acaaaggtat aaaaggtatg gaacaaggtc ttgaaaaaca agatgaggcc atcaaggaac
    10261 ttacaactgc ttataataaa atacctacta cagaaaagac tcctactgat aacgaaaata
    10321 actcaaatga acaaaaccag agtaatacca gtaattctga atcagacgac actaagaatg
    10381 attctacaga aaataagaat aacaattcca gcgagaataa accaaatgta aatgaacaaa
    10441 aagaaagttt aaatgctcaa atacagcaac taaaaaagac aagagatgac ttaaatacaa
    10501 agttacaaaa aaataaaact caaaagaacg aattaagtaa aaaactaaga tcaatggaat
    10561 ctcaaaagaa agagctacaa agcaagttaa ataattctat aaaccaaaag aaagacatgg
    10621 aaaaaagcct tgatacaatg caacagcaaa aaaaatcact ccaagcagtt ttagcaaaga
    10681 cacaagaggt aaaaactgaa ataccaaagg cttttgataa atcaaaattg gattatataa
    10741 attccataga aaataataga acaaaaatag aaaatacact acaatctact ctaaactcag
    10801 gattcaaaca gatgtatata acagtattct gtgtaaatct actagcattt ataatattat
    10861 tgttctataa agaaaataaa actagataaa agagtgttat agtattatat tgtaaattta
    10921 tcttattctt aaatatttta ccctaaagaa taaattcata aaatctttgt aattgattaa
    10981 tataaattac aatataatac tatttttatc ttaatctttt tctcttttac agacaatcaa
    11041 aaagcaatct tagtttatcg aaataaattt atacaatcta ttcatacacc cctttacatt
    11101 tttataacaa ttaaatctat tgttatatat acaaatcaaa ataaaccata tattttcaac
    11161 tataatatgt taaaatcata ttatgagcat attaatttaa gcaaaatgtc atttagactt
    11221 taatttagta gttttatatt ataaatttat atatttttaa tttcaaatgt catttagaca
    11281 ttactcataa aaagaaagga gtgtattttg tggatacttt aggaaaaaga attgcttatt
    11341 tgagaaactc caaaaaactt actcagcgca aacttatgga tattttaaaa tttgaaaact
    11401 taggcaagta tgaaacagga gatagaaaac ctaattgtga tatactaatg tcaatagctg
    11461 attattttaa tgttacgact gactggcttc tctatggtaa agagaaggta aatgtcaata
    11521 gtagtgtaaa agaagataaa gaagattact tacatgttac taatgatgaa atgatgatat
    11581 taaatctata cagacaacta aacgaacgtg ataaaataaa aatagaagga atacttgaac
    11641 ttaagatatc tgaatataaa gacttaaaaa agcattcctc taataataat gaagataaaa
    11701 tggtttaaac atacataatt tgatacgatt ttacatcatc tattaacctt ctttttttgt
    11761 agtatattac taaaatatgg atattggatt atttaaaaac aagtatagtc taatttcaag
    11821 cacatttata atatttagat tatcttatcc atattttagt ttaagtactt tgaaaaatat
    11881 aatacatttc tatgtataat ttatattcct aagaaatgca ttactcatgt ttttaattct
    11941 tccaacatct ttctatacac aattgtacat ccttcataat tcccctcttt aagaaggatt
    12001 gaacatgtac tagaatattt agttaaagta tttaatcttt ccatatttaa gctttttata
    12061 ttactaagtg ctgtaataaa atcatcaatt gctctacaac actctataac cccatctgaa
    12121 tccaatttgc acacaagaat attcacctac tttatatact atttattaat catcttctga
    12181 atattctata ttaagtaatt gataaattga tgatttatat tttttacctg ttcttcttcc
    12241 atttaaaata gctgacaaat agttttcatt aacacctatt ttcttcgcta attccctttg
    12301 agtcatattt aattcaataa gtctttgctt gacttgcact cccaaaaaag gtatccattt
    12361 ttttcgagca tccatttatc aacttccttt ctaagtgatt tatatttact tttatattac
    12421 tttagcttac gcaccccctc tatttagtat atatttttaa tatgtacaca tattaagtat
    12481 ataaatttat ttaatactaa ataagatttt ttgattactt tgtgctaaaa ttaggatgta
    12541 aattcgtatc attaagttaa ttataaagtc attttgacct taagtcaatg gtttttatgt
    12601 cattttgacc tttaatttga aactatttac atcttaaaaa gtctttttga cattaattga
    12661 atggagagtg tttatggata ctttaggaga aagaattgtt tatttgagaa aagcaaaaaa
    12721 ccttaaacaa tatgaactag aagaaatgtt aggctgtgac aacttaagta aatttgagag
    12781 aaacattaga aaaccaaatt atgaaatatt aaaatctata gctgagatat ttaatgtttc
    12841 agtagactgg ctattaaatg gagataatct atcacataaa tcagatttaa tttgcgattc
    12901 ctcctcaaac tatccactta atagcataaa ctctaatgaa ataaagcttt taaacaactt
    12961 tagaaagtta aatgattatg ataaagccaa aatagaaggt atgattgaat taaaacttca
    13021 tgaatatgaa aaagagaagg atttaggaaa aattgagtat aacaaaaata aagatgagaa
    13081 aatagataaa taatttttat gttgcttttg atacgatttt acatcatttc tttgttctaa
    13141 tatttttatt tcatttattt tcttttttat aattttatga taaaataatt agtatatttt
    13201 gctttacttg aatgtaatta tttatacaaa agtcaaacat aatataaatg tagtaaattt
    13261 ttagtttcaa gtaatatctc gttattataa ctaaatattt attaaaatta ggaggataaa
    13321 atgggcttaa gagacaaatt cgcgcaatca tttgctagat caaaaactat gagtggccct
    13381 gagaaaaagg ctaatgaaat aatgggtaaa ttgcttctta agaaagctat attacctatc
    13441 gttttaatgt tcgtgataat aatcgctgga gctatgctta aaatcaattc ttgggttaca
    13501 cttggcataa atttagttat tgctgttggt gctttcttct acataagaaa ttctagtaaa
    13561 aagtatcaga actttaaacc ttatgttggc aatttgatta gtttagagaa aaaaggtaaa
    13621 aaagagtacg ttgctattat aaaacaaggt aagttaccag ttaagttaca aatagcttat
    13681 ggtggtgaag accttgaaca tgttaagaaa aatcaaatgg ttcagataag ttataatcca
    13741 gatgctaaaa tagctatttt ggttaataga caataattta ataaaaaaaa ggagttgtct
    13801 taaaattgat tttttctaat caaatttaag cttaactcct ttttgaattt ttatatgtta
    13861 tttttatata ttatttttat tacttttttt aattaatatt ttaaaatata aaatccttct
    13921 caataaaata gggcactatc tacaatacta ctttatcttt tcctaataat atataatctg
    13981 gtgctactgc tactttcatg ttatacaccg ttttgtaaca taccttttat atcagtaaca
    14041 tcttccttaa tttcctctac atctgttttc attattccca tttgaacaag tatatttttg
    14101 tttatttctt gttgttgtgt agataattct ataaaatttt ctacagtttt tttatacatg
    14161 tctctatctt cttttttctc ctgcatagta tttttaaata atagagcaca tagtatacct
    14221 attgccccca gactactcaa ttcggttatt aatttatcca ttatagcctc ctattgtcta
    14281 aattttttat acttatagct ttaatttttg ttatattctt acatcatgca atattatatt
    14341 ttcaggatta tcaatttaat cttctgtact ttaaattgta ggtttaattt atagattgaa
    14401 taaataataa atatatatac aatttctaat ataacaaaaa ataagtacaa ggcttactta
    14461 ttaaagttaa gaccttgtac tttacataaa aaaaataact aatacatagt tattctaaat
    14521 ttactattca actggtattt ctaattcttt acatctaatt ctaacttgct ctctaattac
    14581 tgctggtact tcttctattg tttttctgcc tcttaaaatt aaagctacat atacttctac
    14641 cattccactc tcacctccag attttcttac actaaaagtg cttatattac ttgcttcatt
    14701 gacagaactt tgacttaaca acatctcata tatttctgca tttgctataa aattatttgt
    14761 tatatcatca tcttgctttg cttgacctat acttatgtct gaaatgtctt tatactgcat
    14821 ttctactttt ctaccttgtt tgatttgttc tattttattt ttatcgaatt taaacaattt
    14881 ttacacctcc attttaagct tgtggctcgt tgttttcagc atcatatcta aaaatcatag
    14941 atggacgttt tatagctgtc catttatctc ctttttctac tatgatttta ggctgagcac
    15001 tttttatgtt ggcttcttct aattcataga tttcttgata acttgagtct gattttttga
    15061 tttcaactag tttcatgtct ttgaataagt cagatttatt tatttttact tgaactttag
    15121 ttttatttat taattgatat atagggtctc caagatttaa atcttcttca agtcctctat
    15181 ctagtgtaat tgtaatatct tgttcattag atgttgtatt aactaccttg tacattctat
    15241 tacctatcat aacataagaa tctttaatta tagaatttcc agtactctct ttaaaaataa
    15301 ataaagatgc atataaatca tcacctttac aataagctct aaatagtatc ttattctctc
    15361 ctattttaat tttatctggt ggtacttcaa aggatatttt ttcagtagtt aaatcactta
    15421 cagattttac actttcacca tttataagca tttctattct acttatatct ttattagggt
    15481 cttgtatttt tatcttaatt gatgaaattg atttttcagt aatttctata gcagcatttg
    15541 tcgttcctac ttctgatata atatataatg tattgtctgt tgcaagtaca tcctttactg
    15601 tatttacatc ttcagaaata ggtactatag taaattcttt taaaaattct actggttgtt
    15661 caattttata tacatcccta ttttttccag taacccatac tgaattatca tttttaagta
    15721 ctaatatatg gtcacttcca taagatttta tttctcttat gtctgttagt gcttttgtaa
    15781 agaacttaat tatagtattt tcactatcgc ttcctaatcc taaagctccc ttatcattat
    15841 atccttgtac aaataattca ttacctgtag ttactactaa tgtatagttt ttaccacaat
    15901 gtacttcttt acagctagat atagttgtaa ttctttgtaa ttcagtaact ggattattgt
    15961 gacctatacc taattgatat tcggaatttt taccaataga ccaaagacta tcatcagatt
    16021 ttataaaata agtaaagtta tctccacatg aaacatgctt tacatctgat acattaatct
    16081 gcacaaattc atccacttga tttatagaca agtcttgcaa taattctcca tggtagtttg
    16141 taccacatcc atacattgtt ccatcattaa gtaatataat aatatgagtc ttagaaatag
    16201 atacttcttt tatatcttca tatggaagct cagtataaag gtctcctttg tataaatttc
    16261 ctaatactaa accataatct atgtcttttg tggtaggtat cactatctta ttgtttagaa
    16321 gtataaagtt atgtctgttt ttaatagatg tcaaatcacc cattacaaaa ttcatatgct
    16381 tataattaaa tattctatta tcattatttc tactatcgta tctatttcca acacctaatt
    16441 gtccatagtt atttaatcca caaatatata tatcattagt atcttttatt gctatatttg
    16501 catattctaa aggaagtatt ttctttactc ttgtattggt ttttataaat gtatctcttt
    16561 ttgttacatc accaacacct aattgtccat tattattaaa acctgtacca tataggtctc
    16621 cattatcact tacataatgt ccacatctat aattcattcc aatatccttt gcctttgatg
    16681 ctattttttt aatcttctct ggagtctcac ttcctgtacc tgaaccatta ctacatttgc
    16741 ctgcagtata tacatcccct tcacttgtaa gtaaaagtac tgcattattt ctgtttggag
    16801 ctacttttac tacatttgaa attggaattt cagaaaatga actttgattg ctattactat
    16861 ttagacctaa gcaataatca gtatttaaac ctgtactata tgcagtatta tagattgtta
    16921 ttatataggt atttatttca tttggtataa cttcttttac attttccata cactttataa
    16981 aactatactg gtcagtagta tttcccatac ctagttgtga attattattg tatccacaac
    17041 aatatgcaat gtttgtgtcc tttacaataa atgttgttgt atctgagcag tacacatctc
    17101 ttacattagt tatattgttt acagtaaatg tcaatacgtc tggatggttg ctttcgcttc
    17161 ctaagcccag ttgcccatga gtgttttctc cacatgaata taatgtgtta tcatctttga
    17221 ttgcatgcga gtgagtagtc cctaaaacaa catctcttac attttggatg tttactttgg
    17281 taaatgtatt tctatttact atatcaccta atcccagttg tcctttggta tttaatcctg
    17341 tggagtatag tgttccatca tttctaattg caaatgttgt gttaccatct atacttgttg
    17401 atattttctt tatattatca aggtttatct gagtgaactt atttctattt attgtgtcac
    17461 ctaatcctaa ttgtccagaa ctattatttc ctgtggcata ggctgttcca tcatttttta
    17521 ctataaacat tgtatttctt gatgttgtaa tttcttttat atcatccata aatataaatg
    17581 agtttgattg ttcagttata tttgtgaatt tttttactgg ttcgttgtta tattccccta
    17641 tcccaagttt gttattctga ttcattcctg ttacatatat attgttattt ttatctacta
    17701 ctactacagt atttcctttt acaattatat cttttatgtc ttttaatcct gtatcatagt
    17761 tacttgtgcc atctatattt gttgaattcc caaattgccc actattagca atcccttttc
    17821 catataactt ggaattacta tgatatatta taaaattact tcctaaatta gaactctcag
    17881 aattaatact agtataactt ctatgtccag taatggttga gccaaatgtt tttggatatc
    17941 cagtaatata tgaacttcct ttcttaaaaa ataatatatt atttgacatc cctattactt
    18001 tttcaattcc actaaaatca gtctcttgta taaaataagt attatgattt gaatttgtag
    18061 cctcaataaa agttccatat cttgaataat cagatatact tggactgcta tttaatgttc
    18121 ttaaatatcc atgatatcct gttaaataaa cataacctcc ttcgtcaatt attgcacaac
    18181 catcataaaa aggaaaaaca ttagttattt taggtaacgt tctaagtcca tttgatgtag
    18241 aagatgttga atttgttcta cattgagata ctaatgcttt gctttggtta ctattatttc
    18301 ctttacctaa ttgataatta taattgtatc cagaaacata tagtttccct tcactgttta
    18361 tcatatatac agaataatca tatgctgcta tcttttcaat attacttatt ccctgcttat
    18421 atggataatt agaagttttt tctatattat tataaggatt atttccccat ccatatactt
    18481 cttttgagtg atttaaagct atgacataat tgcttcctac acaaaaatcc tttacattct
    18541 ctatttctat ttttgtaaat atatttctat taacttcatc tcttaaaccc aattgtccat
    18601 tactatttaa accacatgaa tataatgttc cattattctt aagcacaaaa gttgagtttc
    18661 cgtttccttt tatatccaaa acattatcta tatccacctt agtcataact gacctgttaa
    18721 cagtatctcc caatcctaac tgtccataat tatttaaacc acaagcatac aatgtaccat
    18781 cattcataac aataaacata ttgctatctg ttaactctat cttcttataa tcttgaaaat
    18841 tatcacttaa ctttacattt aaaaattcac tattgtcctt tgtaacatat aaataatcat
    18901 cattaaccaa taaacctgaa taattatcta aatttgaaga ttgcacattt gaaaactcat
    18961 acagaaaagt tgtatgaaaa aatgaaccaa gttgacctgc taaattacag ccagatacaa
    19021 atatctcttt tgaaccattt attaaaaaat taaattgaga accacattta atatctttta
    19081 tattttctac attaacctta gcaaaagtat ttcttgatgc tacatcttta ttggcattgg
    19141 ctagttgacc ataattgttt tgtcctgtac taaacagtgt attatcgtat tttaatatca
    19201 tagtatgatt atttccacaa gaagcatact taacattatt tatactagaa agagtaaata
    19261 caattgaatt attattattt cctgtaccaa gctgaccaac tccattatag cctgtagtat
    19321 acatagttcc atcattctta ataagaattg tatgactact accacacgct atctgtttta
    19381 cattatccat accttctatt ttattaaata cacttttatt taaagtacta ttaattccca
    19441 actgcccatt tgaatttaat ccacatgcat gtgctgtccc atccattttt atgataaata
    19501 cagattgatt ataagtcact acatctttca cactatctat atttacttta gtaaaagtag
    19561 ctctattgtt agtatcacct agtccaagtt gaccacttga gtttaaacct gctgaatata
    19621 atgttccatc atttttaagc acaaatgtaa agtcataacc acatgcaaca gtttttacat
    19681 catctattgg tagcttagta aattcataat atactgtaga ctcaattaca cctaattgac
    19741 ctacattact tgttcctgct ccatatgcaa atccatcttt agttactgct acactgtgtt
    19801 ttgaaccaca agatacatat tttacattat caatttttac tttactaaac atccttctct
    19861 ctgacgaatc ttttccaagt cctagttgaa aacctgtatt atcgccacaa gcatataaat
    19921 taccatctga ttttaaacca aaggtactat tacttatacc agtaaaaaat tctacaaaat
    19981 cataatcatt atatctttta tcaaaatcat caatcaatat gttcttatca ttaaaataag
    20041 caccacgctg taaaagttta ttttgcttca ttattagtac tacctccttt atttatctta
    20101 tttcatatga atatttactt agagttatat ctttttgttc ttttaaataa gtgttactag
    20161 attttgtctc aacaaagtta ttatccatat catgtatata tctaactcta tttgcactaa
    20221 atggtaatac tacatctcta aaaatcacat taccaatttc acctgttata cattttctat
    20281 ttaaatctaa cttaagccct tctatagcta ctatgttatt tccatttgct aatgtatcat
    20341 accaataacc agcctcatct acagttactt ctgactctaa ttctaggtat attaatatat
    20401 ctagcacttt ttcagataat ttgttatagc tattttttaa tactgatata tcagtttgta
    20461 tttgaccact tataacatca tcaaaagata tattttctat ttttttaact acctcattta
    20521 tagctcctga taaatcttta gaatctgtag ttaatatatc tgtatctcca atttttactt
    20581 ttatttcttt tatattttta tcaaacttat catctaaatc ttgaatatcc ttttgagtta
    20641 caaaaaccaa cgtcgggtct acttctaatg taacatttga agtattggac aattgtaaaa
    20701 tcatttttac aaccaaatct ttaatactgc catcttcagc acgtggctta tacgtctctg
    20761 gatacttacc tatagctatt atattattct cattatccag aacagcagct tctcttatca
    20821 taaatccacc aactgttcct ggtaaaatag tctctataac tatccaatta ggattttttt
    20881 catcaattct tagagaattt atatttcctt cccaaactac attctttaaa gcagtttgtt
    20941 cctctgttgg ctcataatat tctccattac tatctccaac ttgaaacttg gttaatccaa
    21001 caagctctcc tagtgcagtt gcatttgcta ttttttctct accaattttg gttaatatta
    21061 tataaaattt ttcttccaat tataccacct ccgatttagg ataaactact acattttcta
    21121 cgcctgtatc atttgcaata gctatattaa tcttacaatt attttcaatg catttaatgc
    21181 ttaaaggata aacctttatt tcttctctac ttaacattcc agctccacag tttacaacag
    21241 cactacattt tataattggt tcaaagtatg gatgaacttt tacctcttca ctacatacca
    21301 tacctccacc acaatagacc atacttttat ctagtattat tggttctaaa tagtgtaata
    21361 agtgagcagg ttttactctt tcaatcatct cgctacaatc taaaagagtt ttatagtcac
    21421 agttatttgc tataaaactc aatacgaatg taaattcatc actataaact tttatatctg
    21481 tttctgattt tgtatatgcc tcacatatac tttttataac ttcaatagta ctagtacctc
    21541 tgcttctcat tttagctttt atattgctac gtcttgtgtc aaaatcaagt tctttttttt
    21601 caatgcagag tatgtcttcc cacatatcta atccccatgt cgctgtatca acaaataatt
    21661 ggttaaaggt atcatcaata tcttctttaa gaatatttaa ttctgtatcg tatgcacctt
    21721 gtatctcctc tacaatgtaa tttctatcaa atgatggtag tttatcaatt agcttcattg
    21781 attttccacc tcactaaaat taacagttgt tacactaggt attttatctt cattaatagt
    21841 tatattatct gtacttccat ttataagtag attacttaaa tcgtgtacac cagtagtatt
    21901 tataagtatt cccatgactt ttgtatagat tatctctcct ctaatatctc taaagtatgt
    21961 atttatactt tctaggaaag attcttttac attgtctaat gtatatccat cttctagttt
    22021 cattactgca cttatactta tttctattgg taatggtgtc acaactgtta tagttggtcc
    22081 aataggcttc tcttcatcta tatgttgctg acacctttca atcgtttctg tatcaacagc
    22141 ttgattattt tccccaaata tcaagacctt aactgttcct ggaccatccc atcttggata
    22201 aaccttaaca ttatagactc catctacttc caaagcccat tcttcatagt gagctttatt
    22261 tccacttgta gcttgatttc tttggatttt ataaaatctt tcttttagtt cttcatctgt
    22321 ctctatatct gtaccacctt caaaacttct tgtgttagta atctttgtta ctccagatat
    22381 attatcaact agtttaaatt cacaatttgt aggtaaatta tatttcttac caacttccag
    22441 agcttgaact gggctattgt caccttcttc actaccaata gttacatctt ttattactac
    22501 aaatagtaaa tctctatatg atattattgt gccattattt attacagttc ctttctctcc
    22561 aataaattca acttctccat ttgactctgt acctaacttt ctatatacac caaattcatt
    22621 gactctttta tcaagaaact ggttatatgt gtcttgtata aaagccattt tatgtatctt
    22681 tgaaagttct agatatatct tcgaaagttc tagattattt ccagatacca tgttgtttag
    22741 aaaagaacct tctcctttat aaatatcaag attaatattt tcaagagttc tattttttat
    22801 tacttcatat gtctggtcac tatacattta tttccacctc cccatagata gtggatactt
    22861 ttacatttgc actcaatacg tctctgttaa agttagcact ttttacgttt acttctaata
    22921 tatatggatt tattagaagg gcctctttta tgaatctact agcttcactt tctgtaagac
    22981 cttttgtata tttttgtcct attagttctg aaagctctgt cccatattct aaagagtata
    23041 tctcatgttc atatctattt gtctttatac acttgtatat ccaaactttt aaggcttctt
    23101 ttttttcaat tattttaaag tcaccttttt ctaaaatagg ttcatctttt tcaaaatccc
    23161 atgccacttc acgaaagatt ggcaattctt ctgttttagg taagatataa tcctctggga
    23221 cacctataaa aggaaatatt gtactcatta taagctcacc aacttactta caacagcaaa
    23281 tttttcgcct attctaaaca ttattaccat atccccagat tcaaagttat ctataaaagg
    23341 attttttact tcatgctggt gttcttgatt tgtttctgtg tcaaatgttt ctagctgtct
    23401 atcaatcatc caactatcta tcaaaatatc ttctttttct aatatgatgt tatttgtctc
    23461 tatttttaaa tctggtaatt tacttttaat ttttccaata aaaaaagaag gttgattatg
    23521 atactttcct tcttctctta ttattcctat aaattcattt attggattag ccattatatc
    23581 accatccttt tataatactc tcctagctgt attaaaatct cttctactac ttaacttact
    23641 tattttaact acatcacctt tttgtggagc atgtaaaaat tctccattac ctatatataa
    23701 tccaacatgg ctgactggat tatgaaaaaa tactaaatct cctgcttgaa gatttttttg
    23761 ttctactttc ttgccttttt tagattgttg atttgacgtt cttggcaaac taacattaac
    23821 ttttttaaaa cagtagacca ttaaaccaga acagtcaaaa ctacttggtc catttccacc
    23881 ccatttataa ggtttaccta gatgtttttt tgcctctgat attactattt tagctttatc
    23941 agatacatta ccagaataac caccaataat aatttttcct tttcttcttc caaatttact
    24001 agcttcttca acatttccaa ataaaatatc tatatggtat gttccatttt tttctatagt
    24061 tattgcaggt cctacgtcat ttactttata gactccatca cgaccagaaa ctcctgtaat
    24121 tacctgtatt tcatctccat agttcaataa aggatgttta tttaaaaact cttttgtata
    24181 ataagtttgc tcatatttac caaccatagg agcagcgcaa gtttttttag atgggtcaag
    24241 ttttttcttt ctacaatctg tatctccacc ttcttctttt ctaggacagt aagctgtaaa
    24301 ttctgctgta aactcttttc ctcctgcata atcttctccc cctaaattac tttcttcctt
    24361 aggttcatcc tgtcctgctg acttttcatc cataagattt tgaaaattaa gttcaagctc
    24421 aatttgatat tctccatttt gccaagtatg tttgtctgta tctatataaa atagtcctac
    24481 aagctttgta taagaatctt taacttttac tcctctacca gttatacaac ttacatctcc
    24541 ataaccttta agagaacagc ttttttctat cccattaaac tcgctatcaa tatctacatc
    24601 ttgattttct tgttgctgaa ttactttttg cattattaca tttacttcct taaaaatttc
    24661 attatctatt ttttcgctaa tcttgcttcc atactggtct accactatta ctttattttt
    24721 tacattttcc atgctctccg aaaaggtggt atttataata ttaaatccct cttcaaacat
    24781 aacacttaat gtaacagttc ctttttcaat aacattaaac ttatctaaat tagcctctat
    24841 catatacttt ttctttgtct ttttacttgc ctctgtatat gcactcatta ttgtgtcata
    24901 accatttact cctataaaca tctttgtata cttgacattg gtctttgcta ttgttccaac
    24961 tgaaagccta ttttcagcaa atacttgctt tgctatgtct tcaactaact tatctttaaa
    25021 attgtatgac acttcacttt gtgtaagtaa aaatcccata tctttagata caaaactaat
    25081 ttcattactg cttgaatctt tagacctatt aattatcatt cctctaaaga gttctttctc
    25141 atctacatag aaacagactg tactagctat tggtatatcg atttgttgga aatttacatc
    25201 aaatgatgat tgtattattg aaaactctag tgtccttgat ggagatttat aatcacctga
    25261 ccatgatacc ttgtctacta tgtcagttat gtcatatata cttccgtttt ttatgtgtac
    25321 ccaaatctta ataataaaaa atcacctgcc ttttcattta attctatcag cagttactta
    25381 caacttatta tctataatag ttattgtttt ggagttactt ttggtatctt tattctttta
    25441 tactctttta aatctaatgt aaaatataca tctcgagtcc catctctttc ttcataacta
    25501 aacccttcaa ttatgacttc catatttata tttgtttccg taattgtaaa tcttaatata
    25561 aaaccttcct tcatccattt ttctatctta tttacacaat catatggtga tggaaaacct
    25621 gtatagtcac agaaagtgta gtctttattt ggaaaaaaac ttgatattga aatgctttta
    25681 agagctacac caccaaaagt tgctacttca cctattttga gtatactaga agagtttaca
    25741 gcagcttttc cattaatact aaaggaagat ggaaatactg gaaatctaaa tctatcttct
    25801 gcttgtctaa gccacatttc cattataaga caccccctaa ctgtgccaat tggatacttg
    25861 caactaattt tgatgttatc ttttctatat cagcttcttc tctaatcact attgtatcag
    25921 ctaacttagc tatgtttata tttccaccat tttttctgct tctatattga tttgcttctt
    25981 gtttagttaa aaccatttca ccttcatgga gtctggtttg atagtcatta taaggaacat
    26041 agttcaatcc aaatgcatta ttacctttct tacttgaata agctttacca gaatcaccag
    26101 cctttgttcc agcatatgga ttatctttgt aatttttttt actcttttcc actagattta
    26161 aaactgttcc tgtgatgctt ccatttttta tcttattaat catagtttgc caccatttgc
    26221 atatatcttt aactatttta catactttat ctaatatgtt aaaaaaagct cccaatattg
    26281 gctctataat tttccatcca gtttccaaca gaggtcctaa ggtctcccat acagttttcc
    26341 aaataacacc cagtgtctct ataaaattgt ttatttgagg tgcatattca cctaaaaaag
    26401 taaaaattct tgtgataata ggactaacag cttcaactac aggtccgatt agttggaatg
    26461 ctgtagttac tattgtacca aatatactta ttaatccttg tgcaattggt gatttattgt
    26521 ttatactatc tagtaaatta tttactaagt ttactactgg aagtagtgaa tctattaatg
    26581 ctgtaaaatc aatattttgt aatagtgtac ttcctgcgtt aacaattgct tttggtgcat
    26641 tccctatcat tgttgctgca tgattttcaa aacgttcttg cttattttca accatcattt
    26701 tacgcttttc agcctcatta gaaattcgat attgagtaga atcagaaaac tcttttccat
    26761 tttttacact tctagattgc atctggtcat atttgttgcc aataattatc ttaccattca
    26821 ttacattgcc tattttacca gccatttttt cagataagcc agctaaattc cctaatccac
    26881 tttctataaa agtttttatt ttctttagtc cttgacctaa aatatcatca aaaggtttaa
    26941 caatactctt caaaccattg ttaatgcttg tttgagctcc tgatattata cctggtattg
    27001 tattactcat ttctttagct aaccccatct gctttacact tatgccttcc aatgcttcat
    27061 caaaagtttt ataattttct cctaccagag tattaagaga ttccatattt ccttttgctg
    27121 cacttaagaa agctgctgat acatcttgtt cactacttgt attcatattt aaagctctaa
    27181 catctaccat agcttgagtt atatcttttt cttttttgcc accagtaata ttactaatca
    27241 ttttagcttt agttcccatt gcaacaacat cttcagggtc aaaaggcgta tcatttgcca
    27301 tatttactaa actgtcataa tacttatcaa catctttttg gctagttttt ccagtatttc
    27361 ttttcattac actttctaga acaactttct gattttgttc ctgaccaagt aagtcaacac
    27421 cctttttcac agcgacacct atagtagcac catttgtcaa atcagatatc ttccctttta
    27481 tcgaaccaag tattccactt gccatatcct ttacagctat tgcaccactc cataccttac
    27541 ctgcaaacaa tctcaatttg ggaaatactt tacttataat gctacttgcc atatctttta
    27601 tagctattac accactccaa aaagtatttt taaacaacat taacttaggc aaaattctag
    27661 ccatagcact acttaccata tctttaagat taatacttcc ttgataagga cgtttctcta
    27721 ctcgttttaa ttttcccaag atactactta tcactctctt agtcatatct tttgctttta
    27781 ttacgatatt ccaaatcttg ccaccaatct tttttaaatt tccaagtaat ttatttttag
    27841 ttttttctac ttcatcactt ttagctttaa atattaatat aatcataata tttgaattaa
    27901 actcattttt cttttgaatc aacttattaa aataatcaat aatcatagct atagttattg
    27961 ctagtctcat aatacttcca tttaaacttc caataactcc tgaaacagca tttatgctag
    28021 aacttaattt tgccatagtt gaacttacac tactagcagt agtttgaact tttcttaacg
    28081 aggaatttaa actgtttact ctactacttg cacttccaat agaatttttt atgctactag
    28141 ccattacatt agtggaaagt gaaacagcca cagttgctct cctaaaatta tcagttactg
    28201 atataatttg gttcattttt ggtgtgtaat tatctgtaag atacatattt ataccttctt
    28261 ctctcacatt tcccattaca taccacctcc tgtcaaagga cacactccat atccatgttt
    28321 cattttttca acttcttcct caatttctaa ggcgaaaaaa gcttcaataa gctttaattc
    28381 gcctttatcc attgcataaa aaagggacgg tcttatacct tttttcttcc aataatagta
    28441 cattattgta gtcttggtat ccgtccctat tagtttttta cctcttctat cactgcattt
    28501 ttaccataac ccataacatc ttgaagtatt ctatataggt tggtaatttc acctggtaat
    28561 aatagttttc ttgccaattc ttttggtgtt ggagccttaa atttatccat tagttcctta
    28621 tttttaaata aaagctttcc atccaaatca caaacacctt ctataattgt aagcatctgc
    28681 atctccggta agtctatatc cgttttcata ttttcatcaa ttttaagaca agtattttgt
    28741 atctcttcat ctttacttat tgttaaagct ctacacatta ctttaaattc ctgcccaaat
    28801 atagttgata atctcttaag ttcaactata gtacttggtc tttctaagtt ttctgtatct
    28861 gcatttaaaa gtaaatctac tatgtttata ttttttgata atccattttc attcattatt
    28921 atttgctcct ttatcaatca aattcaatca actaactttt ttacttatta actacctaaa
    28981 tttattaagt atatatctac taaatatcag tataaaacca aatataaaac taaattatat
    29041 caagaaaatc atactcagta aatgtgaaag gtgcttctat ctctcctttt actccaacct
    29101 cccaatcagc taaagttaaa tcatcaaatg ctatattttt tactgctatt ctttctgctc
    29161 cataagaatc tgggtcattt aatttagata tagcaacaaa tctaggttca gaacctctct
    29221 ttatcttttc tccaattaac ttgtgcattc ttgaactaac atggtataga gttattgaac
    29281 cttttccttt atatcccata tactttgtat cagtacccat ttgacctgct attataatat
    29341 cctcttttgt aaattccatc tttgcttgaa acttctttac ttctgctact ttgtttccat
    29401 caagccaaag ttctccccaa gtaccactca ttacatttct agcttccata tttgccatat
    29461 taataatcct cctatatttc tattgataaa tctatatctt ccatagcatc aagtactttt
    29521 atttttgctt ttaaaaatac tttagaacct gtgttagctt cttttatttc ttgtaatgtc
    29581 atataagata aatctactcc ttttgatttt aaatacgatt tttgtgcttc aaaatctatt
    29641 tcaacagtag aatcagattc tataagtgct gacttttcta attcttctaa ataacttttt
    29701 atagctacta tcaataaaca tttgttgtca taactgttag taacctttcc tatatagtca
    29761 tctattatca cctttcttat gtcactatgt ataatatcta aagtgtcaac tatttttatt
    29821 ttctggaaca tttctccttt ttcttctgtt aactcagtta aagaatttac tcctcttgca
    29881 attcttatag cccctgcttc cttaataagt ataagctctc ctttattaac ccttgattct
    29941 gcatcaactt tcgtcatctt aggtatatca actacatcac taagcttagt ataagttact
    30001 gattgactta aaggtgttcc tgctataagt ccagccaccc tacttgtaaa ctcatcaaca
    30061 ctgtatttct tttctccaac taacacatct tctgtagtaa aattaattat cccttcatga
    30121 tttcctacaa cttttcctaa tacagcttta accttaacct tatctatatc tctaagttta
    30181 attatccaat tttttatagc agtcttatca gcttctactg cttttggcat acatagataa
    30241 ttaaattcct tagtctctaa aaaatctaat gcagtttgaa tatctgcttc tccttctatt
    30301 acataaacta ataatttatt tggagtgtta acatttccca tcaaagctaa atttatatac
    30361 tctttatttt cagcagataa atcaactggt atatcctctt tttcatgtat ttcattaaga
    30421 cctagtgcct tagcatcttt aagcaccatt gcaattattc ctctagctga acgttcttta
    30481 acagttgtag ctagctcctt aaatgatatg ttgatacttg gtaatcctat agccatacta
    30541 tcactctcct aaatttaaat tgatttcttc catattttca tatactacat cttcttcata
    30601 tacactatcc agataattta gtgtaattgt aaagttcaat acataaattg actcttcttt
    30661 tttaatactt tgttctagct ttgttatatt taattttctg tctgttacct ttatatttct
    30721 gttgaataac ttatttaatt catctgaaat ttcatacatt ctaatactct ttgactgtgg
    30781 ttttggtaag tattttatag agataacaat atctttatta ttcgtattta acattatagg
    30841 tgtacaaata actgatagta tacttacaaa aaaacaagac ccttcagagc cttgcacact
    30901 ttcatcatca ataaaaatac tttcagtaaa gttttttttg agaatctcaa taattgtttc
    30961 taatatttcc ttgtatttaa gcaaataatc accacactat cactaaatat tcttgcttaa
    31021 tctgataatc ttactataaa tgatataagt catttttaaa tatacatctc atttattcct
    31081 gtcaaatatc ttctatattt ttaggtaata ttattctttt caatttatca cctccttcaa
    31141 tatggatttc tttcacaata acattatctc acatatttta ttgcctagtg ttgcaatagt
    31201 gtatataaag tgttgtaaag ttgtcatatt ttttataata tctcactcaa tttaaatatc
    31261 atttttttct ttaaattgta gcaagatgtt ctatctaagt gtagtttcat agaaatatat
    31321 gtcatattgt tttttgtctt actattataa aaaagattaa aaaattccat ttctatatca
    31381 tttagacatg ttagagcatt ttctatcttc tctttttcta tttctatatc ttctttttcc
    31441 atttttaatt ttcttaatct ttcctctctt tttaacacct cattttccac agaagaactt
    31501 atgttatatg tgttactagt cttttctgta tataccattg ctccacatcc agagtattca
    31561 ttctcacatc tttttatgtc caaattaata ttctttatct ttatatccaa atacttataa
    31621 ttatgtaatc tttcatctgt ttctttaaat aatatatctt ttttgttttc catagtactt
    31681 ctcctttttt atatacttat tattagcttc aaatttacaa taatctcttt agtgcacagt
    31741 tttttactaa tctcacaact ttcaagaaca tattgttcta taactgtata aaaaaaactt
    31801 cttctatatt ttgttctaaa atactagaaa tcttcttagc cataagaaga ccaggatttt
    31861 ttatacctct ttctatttga gatatatagt catttgtaat tccagccttt ctggcaagtt
    31921 gaatttgtgt catacctttg cattttctat aatatttcaa gttattttta aacattgttt
    31981 tatacctcca ttaccattaa gaatattttg ttcttccaca cttagattat atagaatcat
    32041 ttgttcttta tcaatagatt ttagaaaaaa atattctttt atattttatt agttttattt
    32101 tttagaacaa tttgttataa tctatacata aggtggtgtt ataatgtttg ccaaaagatt
    32161 aagagaactt agaaaggaat ttggattgac tcaaagagaa cttggagaaa aagtaggcgt
    32221 ttcccaaaga gtcttagggt actatgaaac agaaaacaga tttcctgatg aacacatttt
    32281 aaacaaattg gctgatgtat ttaatgtatc agttgattac cttcttggaa gaaccttagt
    32341 taaggaaaat attgatacag tagctgcaca tagaaaaaat ccacatgaag agttacctga
    32401 agaagcacaa gaacaactta atgattatat agaattttta ctaaataaat ataaaaaaaa
    32461 ataaaccata tgatgagcag tctatttttc tgctctttta atataattta aaggcaaaca
    32521 tacattctat tttaggggga tttttatgaa taatttggat aagctttttg aattagcttc
    32581 tcaagaagag ataataattc attataccac ttatattgca ggtgatttag aagggctata
    32641 tataaacaaa catggtatta aaattatatc attactcagt aatttaaaac aaaactcaaa
    32701 aaaattgaca tccatcttag ctgaagaact tggacatcat ttcactagcc ttggatacta
    32761 tgtatcctct tataacgatt attatacaaa aattattata gataagtgcg aaaataaagc
    32821 cttaaaatgg gcttgtgagt tcttaattac tgaagaagat ataataaaca taattaattc
    32881 tggcatcact tgcgtttatg aaatggcaga catacttaat gtggatatta ctttctttca
    32941 aaaaagatta gaatttttat cattaaaaaa acagtcttta caacttggaa ataataaata
    33001 tttaatatta actaatttgc cgtatttcta catatttgat ccaatttcat aataaaaata
    33061 gtagtaattt taactggctt aacagtaaaa tctatatatt tattttaaca aattttggtt
    33121 tctataaatt caatattaat atataataat gatatcatat tatttaaaat tctaaaaaca
    33181 aatatatgct tttgttaaga aaggttgttt gatgaatgta taaagaaaag ttgtttgata
    33241 attattttaa gttcttagca ctattatttt ggccaattat gtggtataag tggatagtaa
    33301 tttcaaatgg tactcttgaa aatatgttat ttaccatata tgcaatcgtc gcaattattt
    33361 tcatcatttt atattcggtg tttatgataa aatataaaga cattacacaa atagatttct
    33421 tttacaggat aagtactcta cttgctttta tatttacact ttttagcttt ttaatatacc
    33481 caaagagttt atttttcttg tatttaaaga ttatttttac tggaatatac ctttattatt
    33541 caatagtaaa aactcttaaa tttaaagatg atgaaggtgt agtgggtatt atgagctcac
    33601 ttttattaat tgtaataact ttgttctact aaatttaatt ctatttgact taatatggct
    33661 ttaaatatta gacatctaca ttattttctg atattattta agatggtagt cttatattaa
    33721 taatgaaata cactatgaag gagttaacta ttatggaaaa aaaagtttta gcaacagttg
    33781 gagaaaaaga gataactaat attgatattg aaaatgcatt aaaatcatta gacccttatc
    33841 aagctatgca atttaaaact gaagaaggaa aaaaacactt attaaatgat ttagtgaatc
    33901 aagaattatt cttcttagat gctaaagaag aaaaattaga tgaagaagaa atatttaaat
    33961 tagaaatgaa aaagatagaa gaaaatgtat taaagcaatt tgctataaat aaagttttat
    34021 ctagtgttaa tgtaacagaa gatgaaaaag taaaattctt tgaagctaat aagagctctt
    34081 tctctaaacc agaaagtgca actgctaaac atatattagt agatagtgat gaaaaagcta
    34141 aagaaatact tgctcaaata aaatcagaag aaatatcttt tgaagatgca gctcttaaac
    34201 attcttcttg cccatctaaa gatatgggtg gtgatttagg aacattcggt agaggacaaa
    34261 tggttcctga atttgaagaa gctgtgttct ctatggctaa aggtgaagta agtgaaccag
    34321 ttaaaactca atttggatac catataataa aattagaaga ccttcaagaa agtacagagt
    34381 ctacatttga tgaagttaag gctgaagttg aaaaaagttt attatatcaa aaacaaaatg
    34441 aagtttatgg aaataagata aatgctctta atgctaagta cggaaacctt gtaaaataca
    34501 acgattaatc ataaggtcat atataaaaaa gctgtctttt cacaatattt attagaaaag
    34561 acagcttttt atattaaagt ttaaatattc agtttcatat tatcattctt ttattgtaaa
    34621 gaactcagct taaaagttta aataagattt ttaattatca ttagctaaat ttttatatcc
    34681 acgtattatc tcataacagt aacctattac atctcttctt tttattatcc caataaatgt
    34741 gtcttggtca tctataacag gaatgaaatt ttgatttaaa gattttataa ctaaatcttc
    34801 aatatctgcg ttgatggaaa caggagaatt atccattttt ctatttatat ctgtcactgg
    34861 aacatcttcc aatcccttta agtccagatt taaatcgttt tttaatgtcc ataaaaaatc
    34921 accttctgtt atagttccaa catatttacc atccttactt attattggaa ttgcagagta
    34981 tttgtgatat tccatttttt ctagtgcttg ccttatagtg tagtcttcat atatatatgc
    35041 tacttcactt tttggtgtta gaaagaataa tatgttcatt acactaccca ccgttactga
    35101 aaacttataa atattactca atatttataa tgttactttt ttgcagtaac tttctccttt
    35161 cttattttct aagtattctt aatactatac gctgtaacaa gcgtttttcc tttgctcatt
    35221 ttaatcttat tatgctttat taatattttt atatggtcta tcagtatcac ttattctctt
    35281 tttagaaaat ttactaaaaa ttaattgact taaacttagc cataataatt ttactctaca
    35341 ctattaattt taacatattt atctcattat taatgatttt attttaataa ttttaattta
    35401 ttttgctatt aattttattt acaaaaactg aaaaaaccgc taaatgatta gcggcttatc
    35461 tctcatatta ctagaacact aatatatatg aatttttaat tataatctaa ctacgttagc
    35521 agcttgagga cctttagctc catctactac ttcgaactca acgctttgtc cttcttctaa
    35581 tgttttgtat ccatctccag ttatagctga gaaatgtacg aaaacatctc cttcaccttc
    35641 tacagatatg aatccaaatc ctttttcgtt gttaaaccat ttaactacac cagttttcat
    35701 taataaatcc tcctaaaaaa acacaaatat ttagctactc ttttctgtaa ccttattact
    35761 actctatcat atatagctat gtttgtcaat tattattatt tttatacatt aataatcaat
    35821 ttttttactt tataatttta aatgattatt atcttttaca ctttattttt ctttataatt
    35881 ttttaataat ttatttttat tatatggaat cttatggact tatcactcca gcctaaatcc
    35941 catggcacca acattctatc agtattatgg caagttacca aaggatatcc tcttgagtct
    36001 aatcctgtca ctgtcgaaac atgtgttatt ctgcctttct tctcataacc aacaaaatcc
    36061 cccggacgta aattataagc ttctttgtat acttcttcat aggaaccttt agcaattaat
    36121 gaacctcttc cactatacag tgcataattt ttaaaagctt gtgcatttac ccatgctttt
    36181 gtacctgctc tctcgctata attccaaatt gagttctttt tgaaccttcc actttcaaac
    36241 attatctgtg atgcaaagtt tgcacaatct ccaccttctg gattataatc cctatagttt
    36301 ggattaaatt tcataccatg ttcttcatca gctgcggctc cacagtattt atgtgcataa
    36361 tctatagcct ttttttgttc atctgtaagc tgtaactcta caccatcttg ttgcccaata
    36421 tagtttctta tatcctcaga ttttatattt tctaaattta aagagtctgc aaaagggtct
    36481 gtgtaccact ctttagttat gacatattga ttatctataa tttttacatt tagataatga
    36541 tatgtaccta ttctaaaggc attttctttg tccttttgat tttcatatga atacttatat
    36601 tcagtcgata ctgaacagat tataccatag aggtcttttt ctttttcttt tattttttta
    36661 atcttaaccc ttgtctttat gtctttaaat tttacacctt gcttactaga ccagttttct
    36721 aaatacttca tcttttttac ttcattttca taagcccaca gaccaaattt tttatccgtc
    36781 tcataaagac cttttaaaat gttttcattt ttctctaata ttgccttatt tctgtagtca
    36841 aataagtttt caagcaatag tttatattgg tcttttacat catcaccttc aacatttgca
    36901 gattccatga ctttttcttc catatttttc atacttaaat cattgattaa aaatacacct
    36961 gctgtagtag ctaaagacaa aaatacaaat gccattatac ctttgatgta cttattctta
    37021 tttttcagta ttttcaaagc tacacctccc aaatagtaca ccttataaat gaaattacaa
    37081 agaagattct gtcatataca ataattttca tatttaataa ttctctattt tattatgtcc
    37141 aatattaaaa attaattgta tcaatacact gtttttttta
//
LOCUS       DAELXX010000026        36743 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.026, whole genome shotgun sequence.
ACCESSION   DAELXX010000026 DAELXX010000000
VERSION     DAELXX010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36743
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.026"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            235..936
                     /locus_tag="KPH29_001282"
     CDS             235..936
                     /locus_tag="KPH29_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HBG1466905.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIINIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDDLCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
     gene            1207..2025
                     /gene="larE"
                     /locus_tag="KPH29_001283"
     CDS             1207..2025
                     /gene="larE"
                     /locus_tag="KPH29_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HBG1466906.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNMDDLGDYRPGLKALSELGVISPLKDSSLKKEE
                     IRSLSKILGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLFNLGFSQFRVRM
                     HGEIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            2080..3228
                     /locus_tag="KPH29_001284"
     CDS             2080..3228
                     /locus_tag="KPH29_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBG1466907.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            3364..4752
                     /locus_tag="KPH29_001285"
     CDS             3364..4752
                     /locus_tag="KPH29_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466908.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYTTLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            4781..5479
                     /locus_tag="KPH29_001286"
     CDS             4781..5479
                     /locus_tag="KPH29_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1466909.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     ENDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            5482..6915
                     /locus_tag="KPH29_001287"
     CDS             5482..6915
                     /locus_tag="KPH29_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1466910.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            6937..7674
                     /gene="truA"
                     /locus_tag="KPH29_001288"
     CDS             6937..7674
                     /gene="truA"
                     /locus_tag="KPH29_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBG1466911.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHNPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     misc_binding    7785..7992
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8245..9444
                     /locus_tag="KPH29_001289"
     CDS             8245..9444
                     /locus_tag="KPH29_001289"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBG1466912.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            9691..10737
                     /locus_tag="KPH29_001290"
     CDS             9691..10737
                     /locus_tag="KPH29_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HBG1466913.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGECLPNDFFFLLSGF
                     GSFCYMKTNKADLKRMVALGDGRTKQMYKFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DGGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDERIYLYDCGRTELLHLSY
                     DKLRESMNCNSPGLSKENTICTIRMNTSKNKYNIAREAIAIKRDMFLNPSKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNDSFIQVADIFDKTAIIIEKISNIIIDYLTQTCDDTEQLPLLFSE
                     VLEHMKTGYLILDV"
     gene            10845..12008
                     /locus_tag="KPH29_001291"
     CDS             10845..12008
                     /locus_tag="KPH29_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG1466914.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFNVLEEFKREHRNKIIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAQAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     SKNPSSTDLQEIAKEITDISKKDYEIFSMYTGDDNWYYMIQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREVQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
     gene            complement(12097..12477)
                     /locus_tag="KPH29_001292"
     CDS             complement(12097..12477)
                     /locus_tag="KPH29_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HBG1466915.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EVESIKTYLGNIIESVKFDVNYIF"
     gene            complement(12480..13139)
                     /locus_tag="KPH29_001293"
     CDS             complement(12480..13139)
                     /locus_tag="KPH29_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBG1466916.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERISIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
     gene            complement(13245..14219)
                     /locus_tag="KPH29_001294"
     CDS             complement(13245..14219)
                     /locus_tag="KPH29_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBG1466917.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGVLVDNEFNTI
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDITGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVEGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYIPYFED
                     ENFELKFDRTPEDIIIYLKQILTILNISKKEIYFKKRSVLVNNSKIINFKNLSSSFKL
                     IKLNEFEEYPDFILKLGDKKSFVESVIKINKSNRKSIKNLILKLSKKP"
     gene            complement(14259..14606)
                     /locus_tag="KPH29_001295"
     CDS             complement(14259..14606)
                     /locus_tag="KPH29_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466918.1"
                     /translation="MLNLKEIREEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILIDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRVIISIDSYDA"
     gene            complement(14824..15780)
                     /locus_tag="KPH29_001296"
     CDS             complement(14824..15780)
                     /locus_tag="KPH29_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBG1466919.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTIYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIQAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
     gene            16221..16589
                     /locus_tag="KPH29_001297"
     CDS             16221..16589
                     /locus_tag="KPH29_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HBG1466920.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNKVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
     gene            17014..17259
                     /locus_tag="KPH29_001298"
     CDS             17014..17259
                     /locus_tag="KPH29_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466921.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVVNKVKTG"
     gene            17358..17621
                     /locus_tag="KPH29_001299"
     CDS             17358..17621
                     /locus_tag="KPH29_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HBG1466922.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(17906..18853)
                     /locus_tag="KPH29_001300"
     CDS             complement(17906..18853)
                     /locus_tag="KPH29_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HBG1466923.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLADTQALATEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(18856..20487)
                     /locus_tag="KPH29_001301"
     CDS             complement(18856..20487)
                     /locus_tag="KPH29_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG1466924.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFMRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            20617..21534
                     /locus_tag="KPH29_001302"
     CDS             20617..21534
                     /locus_tag="KPH29_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG1466925.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            complement(21591..22172)
                     /locus_tag="KPH29_001303"
     CDS             complement(21591..22172)
                     /locus_tag="KPH29_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG1466926.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(22212..22778)
                     /locus_tag="KPH29_001304"
     CDS             complement(22212..22778)
                     /locus_tag="KPH29_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG1466927.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQMNNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            complement(23233..24093)
                     /locus_tag="KPH29_001305"
     CDS             complement(23233..24093)
                     /locus_tag="KPH29_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG1466928.1"
                     /translation="MLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGL
                     GVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMAT
                     LDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIV
                     DCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKK
                     IMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHE
                     ALGNGFKKIE"
     gene            24242..25132
                     /locus_tag="KPH29_001306"
     CDS             24242..25132
                     /locus_tag="KPH29_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG1466929.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLSPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAV"
     gene            25350..27104
                     /locus_tag="KPH29_001307"
     CDS             25350..27104
                     /locus_tag="KPH29_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HBG1466930.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLTMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKSLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVAKNKGAIILTDGKSVPFKTEGLNVYAIGGKSA
                     INEDLVNETKATRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMIKYNNMDVTPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNVKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVSVTA
                     TNINGNISLFGSDNKGTFDGIFKLNLKKEPSNLHLKLVGNGINKLPEGWSKDYILGNG
                     QPIIEVKNNGINNISIGE"
     gene            27488..28474
                     /locus_tag="KPH29_001308"
     CDS             27488..28474
                     /locus_tag="KPH29_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG1466931.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKELKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPMVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     ETSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKNIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            28697..28780
                     /locus_tag="KPH29_001309"
                     /pseudo
     CDS             28697..28780
                     /locus_tag="KPH29_001309"
                     /inference="COORDINATES: protein motif:HMM:NF012587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
     gene            28811..30292
                     /locus_tag="KPH29_001310"
     CDS             28811..30292
                     /locus_tag="KPH29_001310"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBG1466932.1"
                     /translation="MYKEPKYRTILESIKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGVIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENDENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDRSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSVLEIFI
                     NDGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
     gene            30318..31289
                     /locus_tag="KPH29_001311"
     CDS             30318..31289
                     /locus_tag="KPH29_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HBG1466933.1"
                     /translation="MKKVISIGEALIDFIPNQNGCKLKNVSEFRRVAGGAPANVSVVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFMTGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(31545..32147)
                     /locus_tag="KPH29_001312"
     CDS             complement(31545..32147)
                     /locus_tag="KPH29_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG1466934.1"
                     /translation="MEICILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPALWAGKPVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEKHSRQFAKQIYYILDNSIEVDNC"
     gene            32428..33639
                     /locus_tag="KPH29_001313"
     CDS             32428..33639
                     /locus_tag="KPH29_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HBG1466935.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSECGVSKYDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEIELINKISKKHNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIVFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYEFPK
                     QYYKRKDGYIE"
     gene            33707..33904
                     /locus_tag="KPH29_001314"
     CDS             33707..33904
                     /locus_tag="KPH29_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HBG1466936.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILPLYIKQLGVTDVSLI
                     QQYSGIIFGCTFVVAAFFSPI"
     gene            33938..34909
                     /locus_tag="KPH29_001315"
     CDS             33938..34909
                     /locus_tag="KPH29_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG1466937.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFMNALWMYKFTGLFIKDKTK"
     gene            35096..35473
                     /locus_tag="KPH29_001316"
     CDS             35096..35473
                     /locus_tag="KPH29_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG1466938.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
     gene            36004..36663
                     /locus_tag="KPH29_001317"
     CDS             36004..36663
                     /locus_tag="KPH29_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG1466939.1"
                     /translation="MLDAFITIVSSIIEIWSCKKVFDYTSQSKTDLKKLNMYFLFSVF
                     LIISSLYINIGANSRILICIFVMFVFYKSNYKVNIYKCLVISFFYWLILIGINGLSML
                     LVATVSNLDNMNVLLSGSLYRYEAIFLGKIMLGILLYVYKASFKNFKISKKEIYICIP
                     MIANLLSFFVLYDYIINLVKISPERRTAFLVIVLLLILSNISIFVLIHIILKIKIRKI
                     R"
BASE COUNT        14302 a         4153 c         5696 g        12592 t
ORIGIN      
        1 tattattaac catgaacatt tgttttgtgc tatcatattt atataaggga attgtcataa
       61 aaaatatctt gttcatacag ttttatatag aaaaaagaaa tgaatctttt ctttatataa
      121 gtatattttg taaattgcct ctatatttat agtttaaata atagtggttt taaaaatctg
      181 acaacaaaaa taaaactttc aaaaaattaa atctaaaaag aataggagat aaatatgagt
      241 attgaagatt tatatgattt tgaatgtgtt cctttgaaca atgagattga tattgatgtt
      301 ttatttgaaa agatgataga agatggaaaa gaaaaagaat atacaccaat tatagtcatt
      361 gatgaaaaag tagggctatt aagaaaaaat atagatatga ttactaaaga gtatggttct
      421 cttgaaaact atagagagta ttgtttaaca aaatataatg aaattgatgt aaatcaattt
      481 tttgatttta ggaagagtga gatttcagaa ttattaaagg agctaattga tttagaagat
      541 gatttttatg attataaaca ttttagacca attataaata tagatatatt tcatgaatat
      601 aacaaagttt acatagctaa aattccaaca attaaaccat atgaagtatt tgcatacata
      661 cctattggag gatttaatga ttgtcctaga gatgaagaac atatagctat tgcaaaatat
      721 tggtatgaaa agcatggaat ctttccaatc gcaattggat gtgatacagt tcaatattct
      781 gtaaaaaatt taactaaaga tggtaaaaaa tttgatgact tatgtattga attagtattt
      841 tattgtgagg atattattat acaagggtat gaaaccttaa aggctcttaa ggatgtatta
      901 aaacgctcta caatagtgct tttttggtgg gattgatatt ctatcattta gatagtatta
      961 aattgttagt caccaagaat ccacttactt tagttatggg gagtgtgtca aactaggttt
     1021 ttatacacag tgtagcatag taatatgttt aaattacaaa ggttaaaaaa atactttaat
     1081 atttttaata ttataattaa tagatttaaa ggtaattata atttataaaa taaatacttt
     1141 atatgttata atcattaaga atgtaaaaaa ataatatata gttttatagt tcatattttt
     1201 ccagatatgg aggtaaattt tgtgaaggaa aaagaaaaat tagataaatt aaaaaaaatg
     1261 ttacttgaat taggcagtgt tgtagttgct tattctggtg gagtagatag taattttttg
     1321 cttaaggtcg caaaagatac attaggtgaa aatgttatag ctgtgaccat tcatgcaatg
     1381 atgcattcaa gtagggaaat tgaagaggct aaacaatata ctcaaaattt tggcgttaaa
     1441 catatcatat taaatataga aaactttgat ttaaaagaat ttaaagaaaa tggtgttgat
     1501 agatgttatc attgtaaaaa atatatattc tctaaaataa aagaggtagc aaaagaacat
     1561 aatataaagt acatagtaga tgggacaaat atggatgatt taggagacta tagacctggg
     1621 cttaaggcat tgagtgaatt aggagtgatt agtccattaa aagatagtag tttaaaaaaa
     1681 gaagaaatta ggtcgctttc aaaaatatta ggattaaata cttttaataa accatcattt
     1741 gcatgtcttg caagtaggat tccttatgga gttgagataa cagatgaaaa tttaagaata
     1801 gtagaaaaga gtgaagaata tctattcaat ttaggttttt ctcaatttag agtgagaatg
     1861 catggagaga tagctagaat agaagttggg caggaagaac taggtaaatt ttttgaaaat
     1921 aacaatttta ataaagttga tactaaatta aaaatatttg gctttaaata tgttacacta
     1981 gatatgtcag gatataaaat gggaagtatg aatttaaatg tgtagattat tttaaaaata
     2041 aataagtttt atagttaata taaggaaaca ggaggtttta tgaaaaatta tacattaata
     2101 tcgccatgtt tttttggaat ggaaaaaatg ctggctagag agattactaa tttaggatat
     2161 gaaataataa aaacagaaga tggaagaata acatataaaa ctgatgagtt tggaatagca
     2221 aagtcaaaca tgtggttgag atgtgctgaa agagtacatc ttaaaattgc tgaatttgag
     2281 gcaaaaagct ttgatgaatt atttgaaaat acaaaaagaa taaactggtc tagatatata
     2341 ccttatggag ctcaatttcc tatatcaaaa gcttcctcta taaaatcaaa attatacagt
     2401 acaccagatg tacaagccat agtaaaaaag gctatagtag aaagcttaaa gaaaagttat
     2461 ttagaggatg gtttgcttaa agaagataag gagaaatacc ctatttttgt atttatacac
     2521 aaagataaag taaccatttc tatagataca acaggagatg ctttgcataa gagaggatat
     2581 agagaaaagg caaataaagc tccaataaga gagactttag ctgctgggct tatttattta
     2641 actccctgga aagcaggtag agttttagta gaccctatgt gtggttctgg tactatattg
     2701 attgaagctg ctatgattgg tataaatatg gcacctggct taaatagaga gtttatatca
     2761 gaaaagtgga gaactcttga taagaaaata tggtgggatg taagaaaaga tgcttttaat
     2821 aagatagata atgaatctaa atttaagatt tatggatatg atatagatga agaatctata
     2881 gatatagcta gagaaaatgc tgaaatagca ggtgtagatg aatatataga gtttaatgta
     2941 ggagatgcaa ctcaatttaa aagtgaagat gagtttggat ttataatcac taatcctcct
     3001 tacggagaaa gattagaaga taaagacagt gttaaacagt tgtataagga gcttggttat
     3061 gcatttagaa aattaaaaaa ttggtcgtat tatttgataa cttcttatga agactttgaa
     3121 tatgagtttg ggcaaaaagc agacaaaaaa agaaaattgt ataatggtat gttaaaaact
     3181 aactttttcc aatatccggg tcctaagcct cctagaaata ataaataagt tttacatgat
     3241 ttatttgtaa aatataaaat tttattaaaa taagtaaaac attatgatgc cttgaaacgt
     3301 ctaattattg tgaggagatt tttgtgaaca aatttaaaga tttataggat taggaggagt
     3361 taaatgaaag tgttaaagct tattactgta gtagccctga tgatgttatt tgttactgga
     3421 tgtaatataa aaactgaatc tccagaacaa ttagcgaaga tacctgacta tgatgataca
     3481 aaaaaagtat tgtatgatgg tattgaccag ttactaaaac cagattcatc tatgatattg
     3541 ccttctaata caaaagaagt aggaaaaata aataaggtag atttaaacag cgatggaatt
     3601 gatgaattag tagtatttga acaaaaagaa gatttaagta ataatgtgag tcaggtaggg
     3661 tttgtaacct tgagttacaa tggagaaaaa tacgtacttg gagaccattt tttagaaaat
     3721 ggcgaatcaa tagaatatgc taatttttat gatttggact cagatggata taaggaagtt
     3781 atcttattgg ttaaatccaa agacaaaacg aatatgcata tatataaagt agtagataat
     3841 gaaataacaa aaatatatga cttggatgca tcatggcttc aaaataaaga agattttaat
     3901 gacatgaaag taaaaattgg atatatagat ggagatgaca agttagacat attgatgctt
     3961 catttgaata ataagactaa tgaaatgttt gcaagtgttg caaattttga tgggaaaatg
     4021 aaaatcaaag attctgttaa gtttgaaaat gtaaaaaact taagtgaatt atatacaaca
     4081 ttaggtaatg ttgcttcatc tgttggaaat tcttctgcag ctataaaagg tattgtactt
     4141 gatattccta tattgaaaga taataactat attacgcaga tactttatat gaaagatggg
     4201 aaaattaaca aagcttttag tgattatgat aaaactatta ctaaatctta ttatatacct
     4261 gtagatgata ttgacaaaga taagattata gaaataccta tagttgcagg cagtacaggt
     4321 aataataaaa atacttatag ttctaaatca tctgcaacta taagttggta tagatggaat
     4381 ggaaaagaag gtgctgactc aagtttaata tttacaagtc agatatatta taattataaa
     4441 tataatttta aattatttat tccaaataat ttatttgata aaatacttgt tgaacaggaa
     4501 tttgttggag aaaaagcagt atttaaattt tactattttg actattcaga tcgtaagaat
     4561 ttatttaata tagtagtaga gagtaaaaac aaacttgaag atagaaaaaa tacaggtact
     4621 caaaactcta ttgtacttca agagagcgat gaatatactt tcttactagt tgtaaacaat
     4681 gcaaatgaaa tggataagct agatatgact atagacgcat taaaagaata tttctcatta
     4741 atatatggtt aatattatac tcaattggag gttttatttt atgaaggaaa agatacttat
     4801 actagaagat gaaattggta ttagaagttt tgttagtata aacttaaaaa gagaaggcta
     4861 tgaaatagtt gaggcaggta ctggaagaga agctatagaa aaaatgacaa cagaaaatga
     4921 tattacaata gctcttttag atgtgatgct tccagatata agtggtatag aagtatgtaa
     4981 atttataagg gagaattttg accaagtagg tataataatg cttactgcta aagctcaaga
     5041 ggatgataaa atagaaggat ttatttcagg agcagatgat tacatcataa aaccatttag
     5101 tataaaagag cttttagtca gggtttctgc tcttcttaga agagtagcaa aggatgattc
     5161 aagtgttaag tcgagtgaaa tagtttctcc accatttata cttgatatag acaagagaaa
     5221 attatttaaa aatggaaagg aaattgagct tacaccaact gaattttcaa tagtgaaata
     5281 tctaatatca aatgctaagc aatcgttaag tagagaccag atattagacg aagtatgggg
     5341 aactaactat ttatatgatt ttaaaatagt agatgtaaat ataagaagga taagaaataa
     5401 aatagaagat gacccatcaa agccaaaata tatacaaact atttggggtt atggatactg
     5461 ttttagaaag gaagaataat agtgttacat tttgaaggtc tgagaaaaaa agtaataaaa
     5521 aactatttta taataattat tataatggtt actctgtttg aaggattgtt catgttttac
     5581 atacaaaact actattatga ttctgtcaaa caactacttg aatctgaaat aaaatatgct
     5641 gatgaatata atgctattac aatggaaact acaagttttg agaaaaaagt aaaaaatata
     5701 tttgataaac aaccattaac taaaaattca gaatttggaa tttcaataat agataaagat
     5761 aaaaatatta tattagacca atatggtttt aaaagtaaag aaaaagcaaa ctatgaagat
     5821 gttaacaatg cattaaaaga tataaaaact aaaaatttaa caccttatac ttatagaata
     5881 ccagatacag gggagcatgt tatgagtatc tccttaccac ttaaggtaaa taatataata
     5941 gaaggtgttg ttagatatac agtatcatta gatgctattg ataatgcaat attaaagcaa
     6001 gctacatggc ttatattagc aggaatattt atacttatta tagctatact aataagtctt
     6061 aaatttgctg agactcttat aaaaccatta cgagagctaa agaaatttgc aaatgaattg
     6121 gctgtaggaa attataatat aaaattagaa aagatgaaga ttgtagatga tgagattggt
     6181 gatttagcac agacatttga acacatggca catgagattg acaaaagtga aaaattaaaa
     6241 gaggaattta tatcatctgt atctcatgaa ctaaggactc ctcttacatc tataaagggg
     6301 tggagtgaaa ccttaggtta tgaaagtata acaagagaag aactagactt aggtttaggc
     6361 attatacaag atgaaacaga aagacttata aaactagttg aagaattatt agacttttca
     6421 aggctttctt cagacaggat aaaacttcat gttgatatag ttgatgttga aggtttaatt
     6481 gtaggtgttg taaatcaact taaagttaaa gcagctgaaa aagatatatc acttttattt
     6541 gaatttgaaa atgaatttat agagaacatt caaggagata aaaatagatt aagacaggtt
     6601 ctcataaatt taatacaaaa ttcatttaaa tttacgagtc aaggtggtta cataaaagtt
     6661 gtagcttctc aagatgaaga gataacaacc atatcagtag aggataatgg ttcaggtata
     6721 gaaaagcaaa atttaaataa agtgttagat aaatttttcc aagaagatta taataaggct
     6781 ggtagtggat taggattagc aattagtaat gaaattgtaa aacttcatgg tggaagaatg
     6841 aaaatagaaa gtgaaaagaa tgttggaaca aaaataactt ttaatataaa aaataaattt
     6901 gctaaacaag cataaattct tagaaagagg attaagatga gaaacataaa aatgatagta
     6961 gcatacgatg gttcaagata taaaggatat caaaaattag gtgacaataa tatgactatt
     7021 caagaaaaac ttgaaaatgt tttaagtaaa atgactaatg aaacagtgga gataataggg
     7081 tctggaagaa ctgatatggg agctcatgca agaggacaag tggtaaattt tagaactaat
     7141 tgtatggatt ctttggataa aattcaaaaa tatttatatg aatatttacc agaagatata
     7201 gttgtaaaaa ctgttgaaga agttgatgaa agatttcatt caagatacaa tgtaaaatca
     7261 aagacttata tgtataaaat tgacaataat aagtatcaca atccttttat aagaaaatat
     7321 gctactcatg taagtaaaaa attagattta gatagaatga gaaaagcaag tgagtattta
     7381 gtaggagaac atgattttac tagttttgct tcttctaaat ctaagaaaaa atcaaatgta
     7441 agagaaattt attctataaa tataaaagaa gatgataatg ttatagaaat atatgtagaa
     7501 ggaaatggat ttttgtacaa catggtaagg ataatagtag gtgctttgat agatgttggt
     7561 ctaaaaagaa gagctccaca agatattaag tatatgcttg aatcaaaaga tagatgtcaa
     7621 tcatcagata ctgcaccagc taaaggacta tgtctttgga aagtaacata ttagtcaatt
     7681 atataaaact ctctttaaaa taaaaataaa ataaattata tagttgacat aaatggaaaa
     7741 ataaactatt atatatttta taagagagat ataaaacttt atatttaaat cctgtgagaa
     7801 agagagtaac ttctatgtgt tttatagaga gctgaggatg gtggaagctc agcaaataag
     7861 tattagtgaa aagaactttg gaggatacta attgaattta gtatgttagt aggtaatctc
     7921 gccttaagag aaagagtgga taagtttatc ttatcaatta gagtggtaac gcggatatat
     7981 ttcgtctctt ggtttttcca agaggcgttt tttaatatat ttaattatta gtgtttatct
     8041 ggtatgttga acatttacta attgattagc taagtttatc aataagagtg gtaccgcgga
     8101 tatttcgcct cttagtattt taaaatacta aggggttttt ttagtattaa aatgatttta
     8161 tgtgagtcta aattctcaga gtcattcaat attaagaatt tctataaaat aaaaattata
     8221 atttaagatt gggaggaatt aaaaatgaag gaaaaagtag tattagcata ttcaggagga
     8281 cttgacacat caatcataat accttggtta aaggaaaatt atgaagatat agatgttata
     8341 gctgtttgtg gaaatgtagg tcaggaagat aaaatggaag atgtatatga aaaagcttta
     8401 caaagtggag catcaaaggc atatgtggat gatataagtg aagaatttgt aactgaaact
     8461 atatttaaag ccgtaaaagc agaggctaaa tatgaaggaa aatacttatt aggaacttct
     8521 ttagcaagac ctataatagc taaaaaatta gttgaagttg ctcataaaga aggtgcaaaa
     8581 tatatatgcc atggatgtac aggaaaaggt aatgaccaag ttagatttga agctacaatt
     8641 gcagctcttg acccgactat aaaagttatt gcaccttgga gaatttggga tataaaatca
     8701 agagaagatg ctatagacta tgctgaaaaa cataatataa aggtaacagc aactaaagct
     8761 aaaatatatt cagtagatgc taacctttgg catgtttcaa cagaaggtgg agatatagag
     8821 cacctagaaa atgaacataa aaaagatgtt tataaacaat gtgtagaccc agaagatgca
     8881 tgtgatgtag ctgaatatgt agaagtttac tttgaaaaag gtgtacctaa aaaagtaaat
     8941 ggcgaagaat tatctccagt tgcactgatt cataagttaa atgagttggg ttgtaaacat
     9001 ggaattggtg ttatagatat agttgagaat agacttgttg gtatgaaatc aagaggtata
     9061 tatgagactc caggaggaac aatcctttat gaagcacata atattttaga aagtgctact
     9121 ttagacaaag atacattaca tttcaaacaa atggtatctt ataaatacgg agaacttatt
     9181 tacaatggac tatggtattg taaattaaga gaatctatag atgcatttat ggaacaaact
     9241 caagataatg ttacaggaac agtaaaagtt aaactttaca aaggaaatat aaaacctgct
     9301 ggaatattta ctgaaaatgc tctttatgat gaaggaatat cttcttttgg aaatagtgaa
     9361 ctttatgacc acaaagatgc agaaggattt attaatttat tcactttacc attaaaaatc
     9421 agagctatga aagctggaaa ataataaaat aaattattta agagtgtata aaaactattt
     9481 ttaggttttt atatgctctt tttttatttg aatttattaa attatacagt aaatagtata
     9541 aaaaaatata ttatagttcc aaatgactta ataatgtgaa aatataagac ctaaaatata
     9601 aattttataa atacaatagt tattgactct ctcattgtaa gagggtttat actgaatcta
     9661 atgaaaattt atagtgagga gatagattat atgagaaaaa taattgaaat taaacatcat
     9721 tcacatgatt atgagtgtat gtggaatggt atagaagatt tatatataaa taaaaccggt
     9781 gaatgccttc caaacgattt tttcttttta ctgtcaggtt ttggttcatt ttgttatatg
     9841 aaaacaaata aagctgattt gaaaagaatg gtagctttgg gagatggaag gacaaaacag
     9901 atgtataaat ttttagcacc aattgttgga tttgaataca aacatcatga gtttaaaaag
     9961 tatgagcaag cattaaagaa agcaaaatct gaaattgatg gtggatatcc agttatttta
    10021 ggagctttag atatgtatta tctaccatat tataagaagc tatatcataa agaacacatt
    10081 ccttttcatt atatattaat ggtaggatat gatgatgaaa gaatatatct gtatgattgt
    10141 ggtagaacag aattattaca tctttcttat gataaattac gtgaaagcat gaattgcaat
    10201 tcccctggat taagcaaaga aaatactatt tgcacaataa gaatgaatac ttcaaagaat
    10261 aaatataaca ttgctagaga agctattgct ataaagcgtg atatgttttt aaatccatct
    10321 aaaggatttt tgggatataa aggttttgaa aagtttatta aagagttgcc acagtggaac
    10381 actgaattgg ataaagatga ttatgataag attttgacca atatggttat gttttttgga
    10441 acagtaccaa caattccaaa tgcaatcaaa ggaattaaag aaccagatac tgttcaattt
    10501 aaaggtggat ttgataaaat gagtagaatg ctcaacagta ttggggagga atacaaaaac
    10561 gactctttta tacaagtagc tgatattttt gataaaactg ctattataat tgagaaaata
    10621 tccaatatta taatagatta tttaactcaa acatgtgatg atacagaaca acttccgtta
    10681 ttgttctctg aggtcttaga gcatatgaaa acaggatatt taatcttgga tgtataaaaa
    10741 atatattgtt gaccctaaca ttacgtaata gtgtaatatg aaaatatagc ttgaatatat
    10801 tagtaggtat tgatggctgt aaaattaaaa tttgaggtgt ggatatgaga acagtgaaac
    10861 aagtttctga tttaactgga ataagtgtga gggcattaca ttattatgat gagataggtc
    10921 ttttaaaacc aaataaaatt acagatgcag gatatagact ttatgatgat gaatctatta
    10981 aaactcttca acaaatttta ttttttaagg agattgatat tcctttaaga gaggttaagg
    11041 aaataatgtc aagccaatat tttgataagg ttgaagctct aaaaaatcaa aaaaaattac
    11101 ttattttaaa acgaaagaga ctggatgaat tgatagagct tataaatcaa actctaagag
    11161 gagaaggaaa tattgatttt aaagagtttg atatgagcga gtattttaat gttttagaag
    11221 aatttaaaag agaacatagg aataagataa ttaaaattta tggtagtgta gaaaaataca
    11281 atgaatatat agaaagagtt aagtctaatg aagagaaaat tgctaaaatg gctataaaac
    11341 aatatggaac tattgagaag tttgcccaag ctataaaaac aaattttagc agtgatatat
    11401 tgaatttagg agaaaaattt gatagatata aaaatgattg tttaaaagaa aaacatccaa
    11461 aattaaaaga attatataga aagcttgtag aagatttaag taagaatcct tcttcaacag
    11521 accttcaaga aatagcaaaa gagataacag atatatctaa aaaagattat gaaattttct
    11581 ctatgtatac tggtgatgac aactggtatt atatgattca aaattatcta gtaaatccta
    11641 tgtggataga agaagtagat aaaaagtatg gaagtggagc aggcaagttt attggacaag
    11701 tactaaagac ttatctaaga gatagaaaac caaagataaa tacattgtat gaaaaacttg
    11761 tagaagattt gagtagagat tgttcttcaa gggaagttca aagcattgtt gaagaaatag
    11821 acaatgaaat gaaaagaagt aatgaatttt ataaaataga caatggtgaa agatactttg
    11881 attatatgtc agagctttat ttacaagatt ctaactatat aaaagtaact gataagaatt
    11941 atggtgatgg tgcatctaag tttattggag aagcttttaa gatttatttt gataataata
    12001 attgttgaaa atttggaagt aaaaaatatg taagttttat aaaagcacaa aaataaggat
    12061 gtagattagc tccatctata tccttattta aagttattaa aatatatagt ttacatcaaa
    12121 cttaacactt tcaataatat ttcccaaata tgtttttata ctttcaactt cttctaaaga
    12181 ttttaattta ttttcagtat aaattgcttt gtccagaata ttaaacctta atcctatttc
    12241 atcaaagtac tcatgattta aaactttgta ccaatggttt ttttctttat tccaactcgt
    12301 agatattgaa tcagcttctt tttgagcttt ttcccaatct tcattttcta tagatttttc
    12361 taaaacagat atattgtctt tatatccttc tgtaaaatca tatagtttat tgttaacgta
    12421 aaatccaaat aatacaaata agatagtcca tagtataaca aatgttgtag atttcaatat
    12481 taatcaccct ttttattttg aataaatatt ttgtcatttt catctaaaac acaaataaga
    12541 acatctttaa aagtttctat gtgattagat tttaaaatac ccattaacca gttagtatct
    12601 ttttgagcta tgtctagatt tttatttatt attctaccat ccagtataag tgggataggt
    12661 aaatgattaa aagctcttgg aggagtacta ttgtagcttg atgctggtac aacacttaga
    12721 ttaccatcag tctccaaaat agcatattgt acatctttta ggttgaagta tccttgaatc
    12781 ctaagttgtt ctaataattc atctatacta attctttctt ttttaagctc tttatataca
    12841 atttttcctt tatctattaa tacagaaggt ttacctgata aaaatgaact taattttctg
    12901 ctttttaatg ataagaatga aataagagtc tgcatgatta caagaccaga tattgctgca
    12961 acaccattta tgattggtat attattattt tccattggga cagatgcaac ttctgcaatt
    13021 aatagtgtta taactaaatc aaaacagttc atttcagcta tttcaccttt tcccatgact
    13081 ctaagtgcaa ttagcactgt tatgtataat ataatacttc ttattaatac tactgtcata
    13141 tctatcaatt actcctttaa aaaatattta cacttagtta gtatatccta tataaaaaat
    13201 ttaattcaaa gtataaaaaa ccttaaataa ttggattcaa ctatttaagg tttttttgat
    13261 aattttaata ttaaattttt tatggatttt ctgttacttt tatttatttt aataacagat
    13321 tcaacaaaac tctttttatc tcctaatttt aagataaaat caggatattc ttcaaattca
    13381 ttgagtttta ttaatttgaa agaagaagat agatttttaa agttaatgat tttagagtta
    13441 ttcactaata cacttctttt tttaaaataa atttcttttt tacttatatt tagtatagtt
    13501 aatatttgtt taagatatat tattatatct tctggagttc tatcaaattt gagttcaaaa
    13561 ttttcatctt caaagtaggg aatataatat aaaggtgttt tgagagcagg taatttatta
    13621 tataattctt caagaatatc gatattttta ggggagttaa agaaaaagca tttacatacg
    13681 ttcattaaat ccacagcatc tgaaagtgag ttatgtttaa tatctccttc tacagaaaaa
    13741 atttttttca tattcagtaa agatatttgc ttagataaga ttctattatt gtatgtaata
    13801 tgttgagata taaaaggttg aatgtcaatc atcatattaa ataactctaa gcatttacct
    13861 gtatatttat atactctaaa tgaagttaga aggccagtta tatcaaaatc accccaatta
    13921 tatattgtaa catcatcaaa tttacaaaac catttataaa aatcatccat aacatataaa
    13981 agattaggag cattattgat atctagttgg ttcaatttgg ttaaattttt acatttattt
    14041 gaaagtattt tattgtattt tggcttaact aaagagtaat aattatctat tgtattaaat
    14101 tcgttatcaa ctaaaactcc accaatagaa attaattccg caccattata ttcttttggt
    14161 ttatttttat tatcatctag tatattaaat tcaaagtcta taaataactt gtatttcata
    14221 aatctaaagc acatcctcag gtaaattatt tattgagcct aagcatcata agaatcaata
    14281 gatataatga ctctaaacat caaaggttca ttacaacttt tatttatttc ataatttaat
    14341 tcaaataaat cttttaagat ataatttata agtattcttt cccaacccat ttttaaatta
    14401 actttagagt ctattaatat attttctatc tttttaaata tttctatatt tgctggtatt
    14461 atactgagct caatattact tttaaacggg ttagactgta aatctgttat aagtatattt
    14521 tttaattcct ctgtaagttc agaaattaaa ctatcaccta tgtatttttt tatgatttcc
    14581 tctctaattt cttttaaatt tagcaatatt agtacctgct acttacatag tagcttttcc
    14641 tttcctttta atactattat tcatttttga atcttattta gatatatttt aagattaaaa
    14701 gtctttagct atataatttt ttacccattt ttaacttata ttaacatatt acaggcttat
    14761 atctccaccc tgcttacatt gaggatggag tatattgcct attttgataa aaatgatagg
    14821 ttgttatttg cctattcctt tttctgataa tttttctaac atatctttgg tcattttatc
    14881 taaatcataa ctaaacttaa agttccattc ttttactgca ctactactgt ctagagagtt
    14941 aggccaagaa tcagctatac tttgtcttac agggtctaca tcatattcta tcacaaaatc
    15001 aggaatatat ttttttatag aatcagctat ttcttcagga gaaaagctca tagcagtaat
    15061 attaaaagca tttctatgga tgagtttatc cgctggagct tggattaaat ccactattga
    15121 ttgaagtgca tcaggcatat acatcatatc catttttgta ccttctgcta tgtaagattt
    15181 atatctctta ttttttaatg cctcataata tatatcaact gcataatctg tagttcctcc
    15241 accaggaggc gtaacataag aaataagacc tggaaatcta acacctctag tgtctactcc
    15301 aaattttgag tgataataat cacatagtaa ttctccagaa actttagtca caccatatat
    15361 agtattaggt ctttgtaatg tatcttgagg agtcatattt tttggagaat tttcaccaaa
    15421 agcagcaatt gaactaggtg taaaaaattt acaatccaat tctttggcta tttcaaggcc
    15481 atttattaat ccattcatat ttaagttcca agcttctagg ggtttacttt cagcaactgc
    15541 agaaagtaaa gatgctagat gaattatagt atctacatca tatttttttg caatttcaaa
    15601 aaaacgattc ttatctgtaa catctaatat ctcaaatata ccagattcac atactggatt
    15661 accttctttt actctccttg aactagcgat tacattttgt tctccatact catttctaag
    15721 ctttattgtt aattctgaac ctatttgtcc taatgcacct gttataagta tttttttcaa
    15781 ttttacaacc tcctaagggt ttaatttatg tagtactact tatttttatt ttagcaaatc
    15841 ttttgtttaa atacaaagaa ttaaagataa taatatacga cttaattgaa tgaagtgcag
    15901 taaattccta aaaatttaaa gttgagatat aatggtctat tgtataacaa taaaagacta
    15961 gattaaatta ttatatagat tgtgtgacta agtcacagaa agtatagtta atttaatata
    16021 taataaaagc atatcaagta aagctttaaa ttaaagtaac tttatataaa aagtaatata
    16081 aattgataaa attataaatt tatttatagt gaatataggt tggattttat agatacaaaa
    16141 atagtatata tgatatgtag accattagca tgtttaaagt agcaaaacat ataaaataaa
    16201 atttgaaagg aagtataaaa atgacaagac catcaatata tcatagccaa ggatacactt
    16261 gtgcagaggc attaataaag tcttataatg aggaacacaa cacagatata ccaatctcaa
    16321 taggaagtgg tatgggggtt ggaatgaatg ttggaagtgt ttgtggagct gtaaatgctg
    16381 ctgttgttat aatagggtat ttaaatgggc gaaatagtaa tttagatgaa aatatagcaa
    16441 ggaagtattc aagagagcta ttgaataaag taagaaataa atacagctca gaaatatgtg
    16501 tatctcttaa aaaaaataaa gtaacttgtg aagaaataat aaatttcaca tatgatgctc
    16561 taaatgatat gctaaaaaat caaaaataat tctaagaaac aagtatttta cattcgatag
    16621 aatacttgtt tttttatgat atttttaata ggtattagtt tattataatt tttaaatcaa
    16681 ttttattata ttttaattga aataatctac tttgaaaatt actaactgac aaaagaatat
    16741 gattatagta tagaaaaaat gtaaattatt taatattaag tgagagtata tgcttaccta
    16801 tataaaatcg tataaattaa tggctaaatt tataaattat aaagaaatat ataagaaaac
    16861 aaaagaatta attagtataa tataaagtta aaaagtgaaa atttatataa tttatttata
    16921 aagtaaagaa aaaatagaaa tttgtaatga aataaaaagt ttatgacata atatggtata
    16981 attagtaaaa atatagagat tggaggaatt gctttgttta tagatgaaga attagaaggg
    17041 tatatattaa catgtaaaat atctgaagac tttaaaaata tacctgaata tagtgatgaa
    17101 gagttttatg ttacagtcta taaagatgaa agttctgact ctgggtacta tgctttatta
    17161 gaaaataaag aagaaagagt tgtatgggat ggagaagttg ttgccaataa tatttttaat
    17221 aacctttgga ttgtagtaaa taaggttaaa actggataat agtctaatta tatagtattt
    17281 gtaatatttt aaatgaattt ttacatattt gagatgagta aataacaaag attattcgat
    17341 actggaggag taattcaatg gaatatagtt atagtaagat gaacttaaaa aaaggggata
    17401 ttgtagaggt aaatttagag aaacaagcca atgtaatatt gttagaccat ataaattatg
    17461 tgaaatttaa aaatcaaaga aattatgatt actatggtgg atttgcaaag aaaaatccat
    17521 gtagaatgag agttcctaat actggaacat ggtatctagt agtaaatcaa gatggaaata
    17581 gtggaatagt aaacttttca atcaatacaa ttcaaaatta attttagaat attggatatt
    17641 cttacttttt gtacttttta tatagttaaa ataaaattaa taagcaatta ttataaaata
    17701 atgtgggtgc ctcaaaatga attttataat tcacgagaca ctcttttttg tataaactca
    17761 aatatacttt atcatttcaa caatgaaaaa gaggcgtatc tgtgttttga gatagcctca
    17821 tttatcatat atcatatata gaattttaat tattcttaac taaaattttt tccataggtt
    17881 atctgtacaa ttaaaatatt tattgttagc atacagctcc acctgtaagg tgtaaaagag
    17941 cctctagtat ctcttctttt ctttccaaaa cttcatctga taaaagcatt tctataaatt
    18001 tatcagcacc aattctatcg atagttattc caaaacgttc acctgtgtta ccctgttctc
    18061 taaataaaag aattgctttt tcaattaaag tcataagttc ctcttttgaa aataatctat
    18121 caatctgtgt tccaggacga actgattttc cccatttccc tcctatatag actttataac
    18181 cagattcttt ttcttcaata ctatcaaaat tacaactttc tatacactta ccacagttat
    18241 tacatagatt actgtctatt tcaagtttac ctttatctgt taatttagct gctttaacag
    18301 gacagacctc tacaacagca cattttttac aaccaacaca aagctctgaa tcatagtcag
    18361 gtacacgttg accaacaatt ccaagatcgt ttaggtcagg tttaatacag ttattagggc
    18421 atccaccaac accaattttg aacttatgag gtagttttac atcataccaa tttttgtaaa
    18481 attcttcgtg tatttcagta gcaagtgcct gtgtatcagc tagaccatga acacaaacag
    18541 tacctttaca agccacaaca ggacgaaccc ttgatccagt tccacctgtt attagatttt
    18601 cgctagcaat aaattcactt agagcttcaa tatcttcaaa ttttacacca ggtaattcta
    18661 ctgttagtct agtagtaaat gctaactgac cgtttccgaa tttttcagca gcttctgcaa
    18721 ttttttttgc ttgtgatgca tttattacac catttacagt tattatacgt gctgcaaaat
    18781 gttctccatc cctgcttggt agaaaacctc taccttttac ttcctttttt tgttctgctg
    18841 taacattact catatttact cctccattac cttaagattt ggataaaata gtattccacc
    18901 ttcaagaact ttcgtatttg tatagccaaa gcgtcttaag cgattttgcg ccatatagct
    18961 atttttacct ttagcacata taagaagaat tttttcatcc ttagcaagac cttcaatttc
    19021 tccattaatt tcaccaacag gaatataacg taaactagga atagaaggag cttttgaaac
    19081 atcaagttta gtccaactgt caaaatcctc aatttcgtca agtaaaacac tatcaagttc
    19141 tccgttaagt ttattttgta aaacatttac tgccactaca aaagggtgaa tagctgttga
    19201 aaaaggtggt gcatatgcaa ggtccatact ttctaatgaa taaaggtcag cgcctaaagt
    19261 tattgcagta gcagcgatat ctactatttt atcaaccgct ccaggtccca taacttgaac
    19321 acctaataat tttctagtat ttttttcagc aaccattctt ataataaaat tagatgcacc
    19381 tggataataa tgagctttat catcggtagc tatggttaca gagcaaacat tataaccttc
    19441 tttttcagct atttctttac ttagacctgt ttttcctgca ttgagctcag gtaattttac
    19501 tactgtcgtt ccaagaacac cattgtaagt tttagatttt ccagatatat tttgtgcgca
    19561 aattctaccc tcatgatttg cagatgaccc cataggtgac catgtaggtt tatttgttaa
    19621 agaatttttt acaaaagcac agtctccaac tacgtatata tctttatcat ttgtaagcat
    19681 atattcatct gcaataattg tattattagg ggcaaattct aaacctgtgt cttttagaaa
    19741 acctgtgtta ggacggattc caactgacat aaccactaaa tctgttttaa tagctctatt
    19801 tttagtacga agtttttcaa ctttattttc accttcaata cctacaacct ggtcacctgt
    19861 aaaaatcatt atgcctttat cagccatatg attttcaacg tattcagcaa aatcagtatc
    19921 aaatccaggc ataacatgtt cagccatatc aacaacactt acacgtatac ccattgcaga
    19981 aagattttca gcaatttcaa gaccaatgaa acctccacca ataacaacag ctctcttaat
    20041 accgttttca atagaatctc ttagttttat agcatcattt ggagtacgca taaagaatac
    20101 tccaggtaaa tctattccag gtattggtgg ttttattggg tctgcccctg tagcaattac
    20161 aagtttgtca tagcttagat tttctttttc tcctgttgaa agcgttttca aagttacttg
    20221 ttttgcattt ctatcaatag acgtaacttc aactccacaa cgaacttctg ctcctgttag
    20281 tttagaaaaa ctttcaggtg tatttacgat taaagatgat ttatcttcaa ttatcttacc
    20341 aacgtaatat ggaagaccac aacctgcata agaaatatca tcacctttat ttagtattgt
    20401 aacagagcaa ttatcaccca tttcacgttt tatttttgca gcagtttttg ttccagcagc
    20461 aactccacct ataattaata ctttcacaat atatcacact ccaatctact ttataactct
    20521 ataataaaat tatacttctt gacaatatat ttgtaaaata ctaaaatatt aataaaatca
    20581 taagcattta ctaatgtttg ctaaggttgt gataatatga caattcgtag tcttgaaatc
    20641 tttgttaagg ttgctgaatg tggtaaaatg agtgaagtag caagaaatat gtatattacc
    20701 cagtcatcag ttagccaagc tatttcagaa atagaaaaag agtatggtgt taagttattt
    20761 gatagaattt caaaaaaatt atatttaact gaggcaggga aaaaacttct tggatatggg
    20821 aggcatcttt tagctgtaaa tgaagaaatg aatgattgta tgaagcattg tgcaaaaaat
    20881 atccgtataa gagttggggc aacagtaact gttggtacat gtgtaataag cccgataatg
    20941 ttagaattat acaaggtaaa tcctcttata gaacctgaag tatttgtaga agatacaaga
    21001 ctgattgcaa aaaaattact taatagtgaa ttagatattg cgattgtaga aggaaaaatt
    21061 aagcacccag atattgttac aaaaagcatt attaatgata atcttgtact tatatgttca
    21121 cacaagcatg agttctacaa aagagactct ataaaagttt cagaactttc aaatcaacca
    21181 ttaattatgc gtgaattagg aagtggtaca agagcacaat tagaaaagca attaaaagaa
    21241 ttaaaaatcc caatgaatat tagatggtca tgttataact ctgaagctat attaagagca
    21301 gttgtagata attttggcat tgcagttata tccgaattac ttattgagga ttatttaaaa
    21361 aagcatcttt tgtgggcttg tgatattgaa ggtattaact tacatagaac attcgatatt
    21421 gcatatcata gaagcaagtt ttttacagaa aatatttcag cattttttga tatttcagta
    21481 gaatatggga aaaaacaagc gagaaagaag agtagaagtg taaacagttt ataatatatc
    21541 taaataaaaa taagagtata tcaatatact cttattttta tttagatata ttagcctata
    21601 ttaacgccaa aatttttaca caatcctgca agtccatcct caaatccaga acctaaagca
    21661 ttaaatttcc actctccatt atgtctatat agttctgcca cgactattgc tgtttctatg
    21721 ctaaaatctt cacccaattc atatttaata agttcctcgt tagtttcttc attatatatt
    21781 cttatatatg aattttcaac ttgtccgaaa ttttgtcttc ttgtaattgc ttcatttata
    21841 gtaacactaa atgatatttt agcaatattt tgaggtattt tagataagtc tacatttatt
    21901 tgttcatcat caccatcacc aacacctgtt ctattatcac ccatatattc aacagctcca
    21961 gaagcgtgtt ttaaattatt atagaatata aagtctccat cattcgttac ttttccatca
    22021 gttccagtta aaaatgcaga ggcatctaaa tcgaaatcaa atccaccatc atatttatta
    22081 atatcccatc ctagtcctac tattactttt tttagaccag ggttactttt tgttaaactt
    22141 actttttgtc cttttgctaa agttataccc atttaaacca actcctcatt ataaattttt
    22201 tattgtaatt attacttata tgtattaatt aattcacgaa gctcttcatt ttgaccaatt
    22261 cctaaagcat taaatttcca ttcttcattt ctacgataaa tttctcctgc caaaactgtt
    22321 actttaccag aaaagtcttc ttgaagatta tatttaaata attcttgctt ggaatttgaa
    22381 tcaattaatc ttataaaaga gttgttcatt tgaccaaaat cttggtctct ttttttagct
    22441 ttgtatatag aaactccaaa aactatttta tgtacatatt caggtatgtc ttctaaatta
    22501 acaattattt gttcatcatc accttcacct tttccagtta agttatctcc agtgtgtata
    22561 atagctttag aagggtgttt aagatttcca aaataaacca agtcacctct aacattcatt
    22621 ttatcttggt tagtcaatgc tattgcaaat gcatctaggt cataattttt tttacccaat
    22681 ttattcatat cccatcctaa gcctactact agatttttta caggattatt atcttttact
    22741 aagctaactc tttgtccttt ttgtaaacta acactcattt taagttctcc tttttatatt
    22801 tttagtttta ttgatggtat caagtataag aaccatatta gctatattat atacgtattt
    22861 aggctaagtt ggctatattt ataaattatt tattatataa ctacatgaat attttataat
    22921 ttaaacatat tgacattata tgatttaaat tattttgtag ttttataata tttaaatttg
    22981 tatttgattg ttataaagta aagtaagtaa taaagttaga ctcaataatt ctaaaatttc
    23041 taagcacact atctaaatga gaacgtattt ggaaatactt gtataaaaca ataattgata
    23101 catttaattt ttatcttaaa aataacaatt ataatatata aatagaaagt ataatatata
    23161 aatagcaagt ataatataac atataaaaag ttgaatttgt atatgttaat tatacttgct
    23221 atttattgtt agttattcta tttttttaaa tccattacct aaagcttcat gtatatctgt
    23281 aattataata aaagcattag ggtcgacatt ttttactaat ttttttaatt cgacaacttc
    23341 tttcttttca actactacta aaagtacatt tttattttct ccagtatatg caccttctcc
    23401 ttttagaata gttgcacctc tatctaaatc ttccattatt tttttactta tatcttttga
    23461 aaggtcagat ataatagtga atgatttgga ataattaaat ccttcaataa gcaagtcact
    23521 aacttttact aaaatgtata aagccacagt tgaataaagt gcaatttcaa cctgtctatt
    23581 tactatacct gataaaacaa ctacaacgca gtctacaata cccattaaaa caggtatact
    23641 aaatgtagga aaatatttat ttagtaggag tgccattaaa tcagttccac ctgtagagcc
    23701 atttattcta aaaataagtc cttgaccaac tcccattaaa atagaaccag atataattgc
    23761 aagatacata tcatttgtta tatctaatgt agccatattt gcagtaagct taagtgctat
    23821 ggtaagaaaa attattccta aaaccgtttt aaaacaatct ttttttgaaa gtattttgta
    23881 agcaaatatg aataatggaa catttaaaag taaattgaca atccaaagtt ctacaccgaa
    23941 taatgaattt aaaacaactc caagtcctgt tataccacca gcagcgattg catgtggatt
    24001 aaaaaataaa ttaagagaaa tggccattaa tatacagcct acaattaata ttaaaatttc
    24061 tgaaaaagtt attctatctt ttttcaataa catcactcct tatatataga ttataatata
    24121 tattatatag tttttccaat ttttaataaa agaaataaaa atatattata aaaacaattt
    24181 gattggaatt ttcagaaaat taatgctata attaaaagta aatttaaagg gaggttataa
    24241 aatggctaaa ttaactctag aacatataaa aggaaataca tattatattc cactccctac
    24301 aatagtaggt gtatacgtag atggtaaaga tgcaatttta attgacagtg gaaataataa
    24361 ggatacagct agacaagttt taagattatt agaagaacat aatttaagtc ctaaattaat
    24421 aataaacact cattcaaatg cagaccatat aggaggaaat gcatatttga agaatcaaac
    24481 aaaatgcaag attgctacga ctaaaattga aggatatttt acagaaaatc caatattaga
    24541 atcagcattt ttatatggtg gctatccaag taaagcactg aagaataaat ttttattagc
    24601 aaaggaaagt gaagttgatt atattatacc ttcaaatggc aaaattgtag atacagaact
    24661 tgaagcgatt tctcttccag gtcattattt tgaaatgatt ggtgttagaa ctccagataa
    24721 tgttttgttt gttgcagata gtcttacacc agagaatata attactaaat atcatttttt
    24781 cttcctactg gatatagatt cacagtttaa gacactagaa aaattgagaa tgttagaggc
    24841 tgatttcttt gttccaagtc actctgtcaa aactacagat ataaaaagtt tgattaatat
    24901 taataagaag aagatggaag aaataataga taatattaaa aaggtttgtt atgagccagt
    24961 tatgattgat gaagttattg aaaaaatgtg tgatttgtac aatgtcaagc ttgatgctaa
    25021 tcaatatgtg ttggttggaa gtacaatacg ttcttatata acttatttat atgagaataa
    25081 tatggttgaa tatatatttg atggtggtaa gatgatgata aaagctgtgt aaatagtaat
    25141 aattttcaaa ttatatgatg aaattgtttt agtatgactg ttatttgaaa agtaacataa
    25201 aaaaatttat ataaataagc taagatgatt gatataaagt tactgataat tttaaggtaa
    25261 tatatacgtt actataatat tatgttatta tttgtaaata aggggataat ttataagcaa
    25321 attaaagacc ttaatttaca ggaggaatag tgtatattat gaataaaaaa ttcaaaaaat
    25381 tcagtaaaaa actaagtata ttaattttag ttttgactat gtttcttttt aataatttaa
    25441 ctgcaaatgc acagattaaa gaagattcaa taataggaat tgatagatat gaaacagcag
    25501 gacttatagc agatagacaa acttatgata ctgtaatatt agttaatgga gataaaaatt
    25561 tatctgatgg gctttcatca agtggattag ctggagctat taacgcacca atacttttga
    25621 ctaaaaaaag tgaaatacca aaagctactt taaatagact agataataag agtttaaatg
    25681 ttgtaaaaaa agtatatata attggtggtt ataatactat tgaaaattca gtagaaaaag
    25741 atataaaaag aaaaggtatt caagtggaaa gaataaatgg taacaataga attgaaacta
    25801 gttataatgt agctaaaaaa ataaatgaag taagtagtgt aaaagaggta atgcttacaa
    25861 atggatttgt aggagaggct gatgctatga gtatagcacc tgtagttgca aaaaataaag
    25921 gagctataat acttacagat gggaaatcag ttccatttaa gactgaaggt ttaaatgttt
    25981 acgctatagg tggcaaatct gcgataaatg aggatttagt aaatgaaact aaagcaacta
    26041 gaatcggagg aaatgataga tttgagacca ataaaaaggt aatggaaaag ttttataatg
    26101 gagcgacaga tttttatata acaaatggat atcaattagt agatgcacta acattatctc
    26161 ctttagccaa agaaaaacca atcgttttag ttgctgatgg aagtaataaa ggtattttaa
    26221 aaggagctaa aagtataaca aaagttggag gtattgatgc caatatatat aaacagtgtt
    26281 tagatatgat taaatataat aatatggatg tgactccaaa tattttaaaa cacttaactt
    26341 cagttgaagg aaatgaagtt tctgaaatac ttatagaatc agataattta ggtgtggttg
    26401 ttgaaaagac gaataccaat aaatttgagt ttgattacgt aagtgtaact aatgaaaaaa
    26461 attgtacttt ttctgtatat aaagaaaatt cttctaataa tgtaaaaaat ggaaagctag
    26521 ttgtaagtgc taagaaaaaa atagataaga aagatagtga tttgcaagat atgaatggcg
    26581 acaatatgat aaatgcaaat aaagatagga tggtaaatgt gataaaaatt ggagttcccg
    26641 ataaaatgta ttctaatttt aaagtagatg ttgaaagtgg ttcagtagaa ctttataata
    26701 ttaaaggagg agctactgtt gatgtcaatg acggaattgc taacattgtt gataacaatg
    26761 tgacaaatcc tttcaatata aatactaata gtgggatttc aggtgtaaca gctgaaacta
    26821 tatcatctga gataaaattt agagcaaaca atggaattgt aagtgtaaca gctacaaata
    26881 taaatggcaa tataagttta tttggaagcg ataataaagg tacctttgat ggaatattta
    26941 aattaaatct gaaaaaagaa ccatcaaatt tacacttgaa gttagttgga aatgggataa
    27001 ataaattgcc agaaggttgg tctaaagatt atattttagg aaatggacaa ccaattattg
    27061 aagttaaaaa taatggcata aataatatat caattggaga ataaaaacat atgaaaataa
    27121 agtttatata gttttaaaaa ttatagaatt ttaaaatatt tatatatatt ctttaaataa
    27181 aaaattattt tttaagtgat aattaagtat ttatcgataa ggctatggtt gtaaaaatca
    27241 tagccttatt tttatattat aaataaatta gagtatttat caattcatag tagataaaat
    27301 tgaaattaaa tataacaaca aaaaatgaaa attacaatta atttaatgtt aaagactcaa
    27361 ttatttggat gtggaatgga tagtacaagt agtatataaa aagtattgac tggacaaata
    27421 actatatttt attttaaata tataatataa tgtaataaaa tatatcaaat gtagaaggtg
    27481 ataacctatg aaaaaaataa caataaatga tattgctaac ttggcaggag tatctaaaag
    27541 tactgtatca agatatctaa ataataaaga tatcagtgat tctactaaag agaaaataaa
    27601 gacaataata gatgaatatg gatatgagcc gaatgcattt gctcaaagtt tgagagctaa
    27661 aaaaacatac tttattggta ttataacacc gtgtttagat tcttttgtaa aatctaaaat
    27721 tatgatggca atagatgaag aactcaaaga attaaaatat acatcactaa ttattaatac
    27781 taatagaaaa ataaggtcag aaatagatag tatatcaaag ttagctagtc taaaagttga
    27841 tggaataata ctaattggta ccgaaataac taaagaacat aagaatgaaa ttgaaaagtt
    27901 agatatacca atggttgtag ttggacaaaa agttgatggt atcaatagca tagtgaatga
    27961 tgattatgga gcaggatata aaatgggtca atatattgct aataaaggtt acaaaaacat
    28021 tgtatattta ggtgttgatg aaagtgatat atcagttggt ttaaatagaa aaaatggagt
    28081 acttaatggg ttaaaagata aagggtatga tgcaaaagta ttttatactg actttgacca
    28141 agaaacgtct atacaaagaa gtggagaaat gctagaaagt gaaaatccag atataataat
    28201 atgtgcaact gataatatag caatagcaac aatgaaagaa atcaataaga gaggtaaaaa
    28261 tataccacaa gatatatctg tagctggatt tgggggatat gatgtattgt ctattataag
    28321 ccctaaactt acaacaataa agtttgaaaa taaaaatgca ggtaaagtag cagcaaatac
    28381 gatagtgaac ttaatacaag aaagaaaaga accactatta aaggagatta aatttgaatt
    28441 aatagaaggt gaaagtacaa taaataaaaa ttaagataca cattacatat attagtaatg
    28501 tgtatttttt tactaaaaaa actataattc taaaaattag gaaaactttt ttgaaagaaa
    28561 tattgcatct gaaataaatt tatgctaatc tttaattaaa ccggtttcga aaccggttta
    28621 attaaaccga aagaatagca caatgagtgc aaaaataact gattgtaaca aagtatcaaa
    28681 gtaattatta gagtacatag gaaataaaca aaatataaaa ggtgtagcac attgtgtaac
    28741 aagattaaga attgttttag atagtaataa taaagcaaat ataaaattac aaaagaaaag
    28801 gaataaaaat atgtacaaag agcctaaata tagaaccata ttagaatcga taaaagaaga
    28861 attaaataaa ttaagaaata tatctttaaa tgataaatat aaaccactat ttcatataca
    28921 tccacaacat ggactactta atgatccaaa tggattagca tattataatg gtaagtacca
    28981 tgtattttat caatggtatc catatgatgc aactcatgga atgaagcatt gggcttatgt
    29041 aagttctgat gattttgtaa actggaatag agaagatgta gcattaatac caatcgaaag
    29101 ttatgaatct catggagcat attcaggtaa tgcgatagag gtagatggaa aattgcatat
    29161 gtattataca gggaatataa aatatagtgc agaagataga tatgcatatc aaaacttagc
    29221 tataatgaac aaggatggta aaataactaa atatgaaaat aatccaatag taagtgaaat
    29281 accaaaagga tatacaggac atgtaagaga cccaaaagta tttaaaagaa aggataaata
    29341 ctttatgtta cttggagcac aaacaagtga taaaaaagga gttataatag tatatgaatc
    29401 taaaaactct atagattgga attttaaagg agaattaaat gtaaaaaata tagatgaaga
    29461 ttttggttac atgtgggaat gtccagatta tattaatata gatgaaaaag atatattaat
    29521 attttcgcca caaggagtag aacctaaagg atttgattat caaaatatat ataatgtagt
    29581 atatgctatt ggtaatatgg atttagataa tttaacattt gaaatagata caatgaaaga
    29641 actggaaaaa ggatttgatt tctatgctcc acaaacattt ataaaggact cacaaataat
    29701 actatttgca tgggctggaa tgggagaagt tttataccct acagataaaa acaaatgggc
    29761 acattgtcta actgttccaa gaaaattaaa tataaaaaat aacaagctat tgcaaatgcc
    29821 tgtagatgaa ttaattaaat taagatatga tgaaacaagt gggcaaaata ctataaagaa
    29881 taatatcaat attatagaaa atgatgaaaa tgtatatgag cttaatatta atataaaaaa
    29941 tattgacagc aataaatttg ggctggaatt attttcatca caagatgaag gtgtaaaatt
    30001 agaatttaac aaattaggaa acattgtaac tttagacaga agcaacttta aaaaagtttt
    30061 cggtgttgaa tatggaacaa atagaaaaga atatataaat attgatgaaa atactaatat
    30121 caaagtacta gcggatagaa gtgttttaga aatttttata aatgatggag aagtagtatt
    30181 tactagtaga atatttgcta aagaaaattc taatcaaata agggtttata gtgataaaat
    30241 agtagagtat gagtatacta aatttaaatt aaagcaaggt attgaattat aaaaacacat
    30301 tataaggggt agaaaagatg aaaaaagtaa ttagtatagg agaagcttta atagacttta
    30361 tacctaatca aaatggttgt aaattaaaaa atgtatcaga atttagaaga gtagcaggag
    30421 gagctccagc aaatgttagt gttgttgtag caaagcttgg aggaaaatca agttttatat
    30481 ctaagttagg aaaagatgca tttggtgatt atataataga tgtattaaat gaagtaaatg
    30541 taaatactga ttatgtattg agaacaagta aagcaaatac aggacttgct tttgtatcat
    30601 taaaagaaga tggtaatcgt gatttctcat tttatagaaa tccaagtgca gatatgcttt
    30661 tagaagctga tgaggttaaa aaagagtggt ttaataattg tcatatctta catttttgtt
    30721 cagtagattt aatagatagt ccaatgaaat tagcacataa aaaagcaata gaatacgcac
    30781 ttgaaagtaa tagtataata agttttgacc ctaatataag attaccttta tgggatagtg
    30841 agcaatcttg taaaaaggca atatcagaat ttttaccttt tgctcatatt gttaagatat
    30901 ctgatgaaga attagaattt atgacaggag aaaatgatat agaaaaatca ttacataaac
    30961 tctttgtggg gaatgttgaa ctagtattat atacaaaagg aaaagatgga gtagatgcat
    31021 acactaaaaa agtaaaaggt atgtgtaaag gggttaaagt aaatgcaata gatacaaccg
    31081 gagcaggaga ttcttatata ggttcatttt tatacacctt attgtataaa caaataacat
    31141 tgaatgatat aaaagaaatg aaacaagaaa cgttaaatga gtatttagaa ttttctaatt
    31201 attatgcagc tcgtagtaca actattaaag gtgcgataag ttcatatgca acaaaggaag
    31261 agataactga atttataaaa aatttatagt aggaatgagt aataggtttt gaaggaagta
    31321 acatgtaaag agtttttaag gatgtttact atgaaaataa ttaacatata aaaagcaata
    31381 aagaggctgt ctcaaaatag atataagcct ctttatcatt gttgaaatga taaaaaatat
    31441 tttaattatt gttacttttt agaagaatat atatcttatt ttaaataagc aaattatgta
    31501 agatttattt atattatcaa tactttactt ggagtattat gtctttagca gttatctact
    31561 tcaattgaat tatctaaaat ataataaatt tgttttgcaa attgacgaga atgtttttct
    31621 ttttctttat tcataaaatt aggtttacct aaatcacgtt caacaagcat tcctatatat
    31681 tttaactgtg aatgcttaca gtagcgcttc ataccatttt cccaaatatc agcacccttt
    31741 tcaggtctat atccacatgt tgtaataagt gctactggtt ttcctgccca tagagcgggg
    31801 cctttctcat taccatagta cttattcatt ccatacacca atctgtcaag tagtgctttc
    31861 attggagaag tgcagtacca agaataaatt ggagtagcaa aaacaattaa atcacacata
    31921 agaattttct tgaaaatttt ctgaacatca tctttacaat aacatccaaa atcaaataag
    31981 ttatcctgac attttttaca agcaatacat ggctctattt tacaatcata taaccatatt
    32041 acttcaatat ttgaattata attttctaat tcctcaataa aaggttttaa aatagcagca
    32101 gtattacctt ttttcttagg acttcccata agaatgcata tttccatatt gatacctcca
    32161 cagtatttta aaatagtttt tagtgcttaa tatagtttta ggatactatg agaatacgtt
    32221 aacaacaata cactttgtat atttttaata aaattgaaac aaattgtttt atcaataaaa
    32281 gttggaatgt atctataatt aaaaatattt taggtatgtt tttacatata tgtgtgtagt
    32341 taactatggt aatttattgt aaaattatct tataggatta taattgctta atttatgatt
    32401 atatttaaaa tcttggaggt aaaaaatatg tactcaaatg aagactataa atttatagaa
    32461 gaaaatgcac catgttatat atatgatgag actaatataa ttgaaaaatg taaggaattg
    32521 aaaaataata ttttaggttt tgatttttta tattctataa aaacaaatcc atataacaaa
    32581 attgtaaaaa gcattgcaga gcaaggtttt ggagcagatg cttcttcaac taatgaagtt
    32641 atgatttcat cagaatgtgg agtttctaaa tatgatattt attattcatc accaggcaaa
    32701 acagagattg atatagaaac tgttttagat aaatgcataa ttattgcaga tagtattaca
    32761 gaaatagaac ttataaataa gatatctaaa aagcataatc agaaagttaa aataggaata
    32821 agaataaatc ctgattttac tatggatgat gatagtgcta aatcaagcaa atttggtata
    32881 gatatagaag atatgagcag tgtttttaag atgttaagta tgtgtgatta tgtatctgta
    32941 gttgggattc atatacatat taaatctcag gtattggatt ttgaaaagtt aggacattat
    33001 tatgaaaaat gtttttttat agctttaaat ttaaataaaa gtaagtatat taatattgaa
    33061 tttataaatt ttggtagtgg acttggagta gcttatgata atcttagaga aaatccactt
    33121 aatcttaaag agctttcaaa attaactcaa aagattgttt ttactaattc aaacacactg
    33181 aaatcaagac ttcttattga atcaggacgt ttcatagttt gtaaatcagg taattattat
    33241 gttaaaattg tagatattaa agtatcaaga gggaaaaaat atcttattgt aaaaaatgga
    33301 cttaatggat ttatgagacc tattatttct aatttaattg aaaatattac ttgtggagta
    33361 gaattagaag gtcaagagcc attatataca tcaaatcatg ccttttctct ttcagtatta
    33421 aataatttaa aagaagaaga gtatgtaaat gttgttggga atctctgtac atcattagat
    33481 gtaattgcaa ataatgttaa attgaaaaaa gcaaatatag gagatttgat ttcaattagt
    33541 aatgctggaa gttactccta ttctttatca ccactattat tttcgagtta tgaatttcct
    33601 aaacaatatt ataaaagaaa agatggatat attgagtaaa caataggata aattaataaa
    33661 cttagtatta ttgaaaaaat ttaaaatgca gggggtgtaa ttctatatgg aattgtggaa
    33721 aagaaattta tttgtatgtt ggataggtat gttttttagt tctataggga tgagccaaat
    33781 agcacctata cttccattat atattaaaca gttgggggta actgatgtta gtttaataca
    33841 acagtattca ggtataattt ttggatgtac ctttgtagta gcagcattct tttcgccaat
    33901 ttgagggaaa gctgctgata aatatggaag aaaatcaatg cttttacgtg caagtattgg
    33961 gatgggaata gttatattta ccatggcgtt tgttcaaaat gtttatcagt tattaggatt
    34021 aagaatactt caaggagttt ttacgggtta tgctactgca tgtactactc ttattgcaac
    34081 acagacagat aaaaatcatt caggatgggc tttgggtact cttgcaactg catcaactac
    34141 tggctcatta attggtccaa cagtaggagg atatatagaa tcaatcttgg gtcttaaaag
    34201 tacatttatt ataacaggag gattgttgtt tgtatcattt atcatctcta ttttatttgt
    34261 agtagataat tttaaaccta aagaaataaa agaatcaatt tctataccta aagaacaatt
    34321 aaatatattg ccaaataaat ttttaattgc atctattttt gtaaccacat tcattactca
    34381 actagcttta tactctatag aaccaattgt aaccatatat atatcacaac ttacaaattt
    34441 tgcatcaaat gtagctttaa tagctggatt gacattttct gcatcaggat tagcaaattt
    34501 attagcagct caaaaacttg gaaaaatgtc agataaaata ggtcctcaaa aagtattgtt
    34561 aatttcactt ttatgggctg ggattatatt tataccacaa gcttttgtaa aaactgcatg
    34621 gcaactgatg ttgttgagat ttttattagg gttgtcagtt gcagggctta atccttctgt
    34681 taacagcttg cttaaaaaaa ttgcaccaga agaatatgtg ggaaaaatat ttggatataa
    34741 tgcatctgcc cagtatatag gttgttcatc aggagcattt ttagggggac aaatatcagc
    34801 acatttaggt attagaacag tatttttttc aacaagttta ttgttgttta tgaatgcact
    34861 ttggatgtat aaatttactg gattgtttat taaagataag actaaataat catactattt
    34921 acatttctaa ttttatgatg taaaataaat attttttatg taatttatca tagaattata
    34981 taaaaaataa ctctatggta aaaattggaa aatattatta tcgacaaaat ctttgactta
    35041 aaaactatat tgcgatagta ttaatttaat aaaagtttga gaggggcgta aggttatgac
    35101 aatttcaaaa ataccacaag cagaattaaa agttatgaag tttatttggg aaaaaaatga
    35161 tacagtaaca tcaaaagaaa ctattgaagc tatggaacga aaatatggtt ggaaacaaac
    35221 aactacattg acacttttat caagacttgt taaaaaacat tttttaagtg cagaaaaaat
    35281 agatagatac actcattata caatagttgt tggacataag gagtatttaa gtgttgagac
    35341 aaaagatttt ttaagcaata tacacgacaa ttctttacag agtttaattt cagcactaca
    35401 tgatgatgaa gttgttgata aagatatgtt ggatttattt gaaagtcatt ttaaaaattt
    35461 agaagaagag taattatatc aggataaaac tctaagaatt taattaatct atggctgaaa
    35521 accataaatg gtattattta atcagctact cactattcat aaagtaatat taaataggtt
    35581 ctctaattag taaaatacct aaaataattg gaatagggag tatcaagtta aatgatacca
    35641 cttatttaaa taagaaatga tttttaaaat ttaaaataat tttataactt gtcaatattc
    35701 attaatctaa agtattattt ctatttaacc atatcaaaat atattttact atttttaatt
    35761 attacaatat aaacctgtct tttgttatta taggtaaact aaaagtaaga aatgattttt
    35821 atatttttaa actttattat tggtaactta tctgtaaata ttttaaaatg tactatagaa
    35881 agttgttaaa aatattaaat aattatatta aatttaagtg tgttttttat gctataatta
    35941 gtatgtattt tttatgatat attttataaa aaatataatt taatcatttt tgaggatatt
    36001 attatgttag atgcatttat aacaattgtt tcttctatta tagaaatatg gtcatgtaaa
    36061 aaagtttttg attatactag tcagtcaaag accgacttaa aaaaactaaa tatgtatttt
    36121 ctcttttctg tttttcttat tatttcttct ttatatataa atataggagc taatagtaga
    36181 atcttgatat gtatatttgt aatgttcgtt ttctataagt ctaactataa ggttaatata
    36241 tataaatgtc tggtaatctc atttttttat tggttaatat taataggtat aaatggatta
    36301 agtatgttat tagtagcaac agtaagcaat ttggataata tgaatgtttt acttagtggc
    36361 agtttatata gatatgaagc aatattcttg ggaaagataa tgttaggtat attactttat
    36421 gtttataagg cttcttttaa aaattttaaa ataagtaaaa aagagatata catatgtatt
    36481 cctatgatag caaatttatt gtcatttttt gtactttatg attatattat taatcttgta
    36541 aaaatctctc ctgaaagaag aactgctttt ttagtaatag ttttactatt aatattatct
    36601 aacatttcaa tctttgtatt aatacatata atacttaaga ttaaaattag gaaaataaga
    36661 taaattttaa tacgattcat ttaaattaaa agttaataat tactttttta tgtataattt
    36721 tacatgaatt tctataaaag tta
//
LOCUS       DAELXX010000027        35941 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.027, whole genome shotgun sequence.
ACCESSION   DAELXX010000027 DAELXX010000000
VERSION     DAELXX010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35941
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.027"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(30..872)
                     /locus_tag="KPH29_001318"
     CDS             complement(30..872)
                     /locus_tag="KPH29_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG1466940.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEISTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVIS
                     VLNL"
     gene            complement(1119..1613)
                     /locus_tag="KPH29_001319"
     CDS             complement(1119..1613)
                     /locus_tag="KPH29_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBG1466941.1"
                     /translation="MSTLVTLLGLLVCTKISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKLSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPRMPLSVSLALAAVISSTDLVAVKSITQGLNFPKNMMSILEGESLLNDDDRII
                     NIME"
     gene            complement(1765..2736)
                     /locus_tag="KPH29_001320"
     CDS             complement(1765..2736)
                     /locus_tag="KPH29_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBG1466942.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKEIKCVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPDEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTRALMSAVPVQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISRNHFVACHLV"
     gene            complement(2737..3714)
                     /locus_tag="KPH29_001321"
     CDS             complement(2737..3714)
                     /locus_tag="KPH29_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1466943.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMVIRGNEISMIFQEPMTS
                     LNPAFTVGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPVLGKNKDKRLYSIPGQV
                     PNPIGIPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            3933..5492
                     /locus_tag="KPH29_001322"
     CDS             3933..5492
                     /locus_tag="KPH29_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG1466944.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSSGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGEPKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKRKEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            5598..6557
                     /locus_tag="KPH29_001323"
     CDS             5598..6557
                     /locus_tag="KPH29_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1466945.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPVYIRYLNWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLVKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            6560..7372
                     /locus_tag="KPH29_001324"
     CDS             6560..7372
                     /locus_tag="KPH29_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1466946.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIIASFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLVDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVISLTIISKKFSSNT"
     gene            complement(7587..8489)
                     /locus_tag="KPH29_001325"
     CDS             complement(7587..8489)
                     /locus_tag="KPH29_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG1466947.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            8844..10022
                     /locus_tag="KPH29_001326"
     CDS             8844..10022
                     /locus_tag="KPH29_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBG1466948.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTIAVKEAISRVGLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINSGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQNKAENAIKNNRFKEEIVPVDVLIRRGKIETIDKDE
                     YPKLGMTFEGLAKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            10031..10687
                     /locus_tag="KPH29_001327"
     CDS             10031..10687
                     /locus_tag="KPH29_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HBG1466949.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIVEGGNL
                     "
     gene            10687..11352
                     /locus_tag="KPH29_001328"
     CDS             10687..11352
                     /locus_tag="KPH29_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HBG1466950.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     ILGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            11457..12233
                     /locus_tag="KPH29_001329"
     CDS             11457..12233
                     /locus_tag="KPH29_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HBG1466951.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALNNT
                     ITELQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            12295..13629
                     /locus_tag="KPH29_001330"
     CDS             12295..13629
                     /locus_tag="KPH29_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG1466952.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVLLNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKVGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSKLEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            14126..15052
                     /locus_tag="KPH29_001331"
     CDS             14126..15052
                     /locus_tag="KPH29_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HBG1466953.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PDNNKPESNKPEDNNNSGSTNKPDNNKPESNKPEDNNNSGSTDKPNNNKPESNKPEDN
                     NNSDSTNKPDDNNNSGSTSENFSAYQKEVVDLVNIERAKAGLNPLTLDSSISNVATKK
                     SQDMIDNNYFSHNSPTYGSPFDMLKKFGISYKTAGENIAMGQKTPKEVVNAWMNSEGH
                     RKNIMNPNFSKIGVGVAQKSGGSIYWTQIFVG"
     gene            complement(15444..18983)
                     /gene="nifJ"
                     /locus_tag="KPH29_001332"
     CDS             complement(15444..18983)
                     /gene="nifJ"
                     /locus_tag="KPH29_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBG1466954.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHACIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRIQV
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKVDVMPANT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMDICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVLEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
     gene            complement(19501..20070)
                     /locus_tag="KPH29_001333"
     CDS             complement(19501..20070)
                     /locus_tag="KPH29_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBG1466955.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
     gene            complement(20142..20750)
                     /locus_tag="KPH29_001334"
     CDS             complement(20142..20750)
                     /locus_tag="KPH29_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG1466956.1"
                     /translation="MDSNDKKGLKSTEIIIINGSPRINKNCSFIANKVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRNKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            complement(20758..22521)
                     /locus_tag="KPH29_001335"
     CDS             complement(20758..22521)
                     /locus_tag="KPH29_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HBG1466957.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDTIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNETIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMKDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            complement(22564..23031)
                     /locus_tag="KPH29_001336"
     CDS             complement(22564..23031)
                     /locus_tag="KPH29_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466958.1"
                     /translation="MKMLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKI
                     DSVFFKLLKLAFGSILLMIAFNLNMILGIGVFLIGVVYILYKRNMEEQVKEHINNVKN
                     NIDISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
     gene            23214..23675
                     /locus_tag="KPH29_001337"
     CDS             23214..23675
                     /locus_tag="KPH29_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466959.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQHFISSILNEKIVNETNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQIT
                     QLKNLLSNTGYNLLIPYKYLTARDIEGIVTLINTGSIELGNERIYNYISKA"
     gene            complement(23768..23938)
                     /locus_tag="KPH29_001338"
     CDS             complement(23768..23938)
                     /locus_tag="KPH29_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466960.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
     gene            complement(24127..24348)
                     /locus_tag="KPH29_001339"
     CDS             complement(24127..24348)
                     /locus_tag="KPH29_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBG1466961.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            complement(24432..25658)
                     /locus_tag="KPH29_001340"
     CDS             complement(24432..25658)
                     /locus_tag="KPH29_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG1466962.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTRFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINANTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            complement(25720..26652)
                     /locus_tag="KPH29_001341"
     CDS             complement(25720..26652)
                     /locus_tag="KPH29_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBG1466963.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIGAVMVLGVGLSRLYLAVHWPIDVIVGWMLGIFFTVIF
                     IKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNISD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            complement(26741..27268)
                     /gene="hpt"
                     /locus_tag="KPH29_001342"
     CDS             complement(26741..27268)
                     /gene="hpt"
                     /locus_tag="KPH29_001342"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBG1466964.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            27497..28573
                     /locus_tag="KPH29_001343"
     CDS             27497..28573
                     /locus_tag="KPH29_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG1466965.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            complement(28660..29868)
                     /locus_tag="KPH29_001344"
     CDS             complement(28660..29868)
                     /locus_tag="KPH29_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HBG1466966.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(30029..30418)
                     /locus_tag="KPH29_001345"
     CDS             complement(30029..30418)
                     /locus_tag="KPH29_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HBG1466967.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVIIKKDKHSNIIENVEFSIKDEEE"
     gene            complement(30522..31523)
                     /locus_tag="KPH29_001346"
     CDS             complement(30522..31523)
                     /locus_tag="KPH29_001346"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBG1466968.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     misc_binding    complement(31582..31837)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(31971..33161)
                     /locus_tag="KPH29_001347"
     CDS             complement(31971..33161)
                     /locus_tag="KPH29_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG1466969.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEINRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDIVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLVRKGFKH
                     TPLQSLCPEYKDKIITCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     gene            complement(33442..34563)
                     /locus_tag="KPH29_001348"
     CDS             complement(33442..34563)
                     /locus_tag="KPH29_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HBG1466970.1"
                     /translation="MGTNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGVAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMQFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTAVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            complement(34624..35109)
                     /locus_tag="KPH29_001349"
     CDS             complement(34624..35109)
                     /locus_tag="KPH29_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBG1466971.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            complement(35111..35779)
                     /locus_tag="KPH29_001350"
     CDS             complement(35111..35779)
                     /locus_tag="KPH29_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466972.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLASNFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
BASE COUNT        12216 a         5945 c         4424 g        13356 t
ORIGIN      
        1 ctatacattc tgtattaaac tactctttat tataaattca gtacagatat aactcttttg
       61 atatgtaatg ttaaataaag tagttcatct tgacttacat agtaatcata attcttttct
      121 acatgagttt ttattttata tgcacaatca taatcctttt catactttac tttaattata
      181 tctataattt cattatttgt atcattgatt ttttcttttt ttactaatcg ttttgcaaag
      241 aattttaaat gagtcaaaag cctatcataa tttatatcat cttctttaaa ttcaactcta
      301 taataatatc ttataatatt tagtatgtct tttacaattt tagtaattaa cagtgattcc
      361 tttgaacttc ccttatagtt tgaatttact atatgcattg ctataaaacc agcttcatct
      421 actggaaatt tcacatcaaa ttctttattt atataatcta cagcccattt accaatctca
      481 tattcttttt tatgtattct ttttatctca tgtagtaaat cattctttat agttatattt
      541 tcatgatatc ttttaattgc aaatgcaata tggtcagtaa gtgctacata aatatattca
      601 taaagcttag tattcaaatt ctcctctgca taagcaatta tttcagtact aatctcaaaa
      661 aatttctcat caacctggct tgctagtttg ctaattttat ctaaaaagct ttcatcttgt
      721 attacaaaag ttctttcaat tttactttta tctatttctt gacctttctt tttttgaaac
      781 ccaatcccac atcctgttag tattaattct ttttttatat tttcatctaa aactaataca
      841 acattattat tataaatttg ttgaataatc ataatcaata tctctcccaa aatacaattt
      901 cccttattca taaaaaaata cctaaattaa gatacaattc acctgtatac cttaacctag
      961 gtattgcctg cttaccagta acaatccagc ttgttaattc ttatatattg tattcaattt
     1021 atggttttat tctattataa ataaatatat atgtcaagta aattattgaa aactatttcc
     1081 tattttttag tattattttt aacatcaaaa atcaaatttt attccattat atttatgatt
     1141 ctatcatcat catttaataa agattcacct tcaagtatac tcatcatatt tttaggaaaa
     1201 tttaatcctt gtgtgattga ttttactgct actaaatcag ttgaagatat tactgctgcc
     1261 aaagcaagtg aaacagataa tggcatccta ggaataagaa aatgtataaa gattccacct
     1321 gcaaacacgg ttatcaaaac taaaccaaat gcaagaagca aaattggttt tctaagctct
     1381 agtaactctt ttcttgatac attttgtcct tcactgaata ataatggagc tatgatacat
     1441 attacaaata tttctggatt aaatgaaaat gataatttaa acggcaaaat agacaatata
     1501 atccctagta caatttgata tattgcaaga ggaatattac tccacttttt gctaaataca
     1561 gttgaaatct ttgtacaaac caataatccc aacaatgtta ctaaagttga cataactacc
     1621 tctcttttca ataataatat tataaattaa acttatatat caactaactt aatattaaca
     1681 acatgaaatc ccatcttaat caaaaaaatt ttcagtttat tctattattc ttattatata
     1741 catcaaattt aatttttgaa atatctatac tagatggcat gcaacaaaat gatttcttga
     1801 tatttctttt aaaactggaa tctctttttt gcatatttcc ttacaataag ggcatcttgt
     1861 atgaaactta catccacttg gtggatttgt tggagatggt atgtctcctt ttaatataat
     1921 tctatttttc ttaattgtag ggtcttgtac tggtactgcg ctcataagtg ctcttgtata
     1981 tggatgttga gaatgtaagt aaagttctcc tttagttgca atttctatta tagaacctag
     2041 atacataaca caaactctat gtgcaatatg atttactaca ctcaaatcat gagatataaa
     2101 taaatatgag agtttatatt tatcctgtaa atccataagt aaatttataa tttgagattg
     2161 aatagaaaca tctagtgcag aaacaggttc atcacaaact ataaattctg gatttaatgc
     2221 taatgctctt gctataccta tcctctgtct ttgacctcca gaaaactcat ctggttttct
     2281 atttaaatgg tacttagaaa gaccacaact ttctacaaca tttaatatat tatcttttat
     2341 ttcattcttt ggcactaaat tatgttctat aactgcctct gatattattt gttccacatt
     2401 taatcttgga tttaatgaac tatatgggtc ttgaaatatc atttgcattt tgggtcttaa
     2461 ctttctaagt tcttccttct ctaacttata tatatctaca ccattaaatt caactttacc
     2521 ttcagttttt tcaattaatc taaggatggt tcttcctgca gttgattttc cacatcccga
     2581 ttctccaact atagccaaag tttctccttt ttttaaatca aaagaaattc catctactgc
     2641 ctttacacat ttaatttcct tatcaaataa tccagattta acttcaaagt actttttaag
     2701 accttctact tttaataaac tctcttcttt tttcaattat atctcctcct ttttatataa
     2761 ccaacatgca atactatgac cactattcaa ttctattaaa ggaggtatct gagttctaca
     2821 tttatcacat actttttcac atctatcact aaaataacat gaatctggta ttccaattgg
     2881 atttggtact tgtcctggaa tcgaatacaa tcttttatct ttgttttttc caagaacagg
     2941 ctttgatttt agaagtccaa tagtataagg atgttttgga tttttaaata attcaacaac
     3001 ctctgccact tctataattt taccagcata catcactaat actttgtcac acatttctgc
     3061 aactactccc aaatcatgtg tgatcatcat tatagatgtg tttaattttt cttttatatt
     3121 tttcattata tctaaaatct gagcttgaat agtaacatct aaagctgttg ttggttcatc
     3181 agctataagt aattttggat tacatgataa agccatagct atcattattc gttgtctcat
     3241 accacctgat aattcatgtg gatatgaatt atatatttct tcagctcttg gtatttctac
     3301 cagttttatc atatcgatta cctttttctt agcttcttcc tttgataaat cttgatgtat
     3361 cataatacct tcaatgattt gacttccaac agtgaacgct gggtttaaag atgtcattgg
     3421 ttcttgaaaa atcattgata tctcattccc tcttattacc ataagttcat cttcattcat
     3481 ctttaaaatg tctttgcctc gaaagattat ttctccacct tctatttttg atgttggaag
     3541 taacttcatc aaagacatgg ctgttacaga cttaccacat cctgattctc caactattcc
     3601 aatagtttca ccttcattta tattaaaact tacttcattt acactcttta caatcccttt
     3661 atctgtatga aaataaactt ttaaatcttt aacctcaatt agtgctttac tcattatttt
     3721 ctcctctcag caaaattact taaaataaca tacacctttt gattgtattt tataaaaatt
     3781 taaattacaa cattttctta cattttatta tttttttcta ttaattataa taatataata
     3841 gttaatttct atatatattg atttaataat gattactatt atacaatgaa aaatatgtgt
     3901 taaatattat tttatggagg ttttataaga atatgaaatt taaaaaattg gcatcattaa
     3961 tcttagtttc atcattaatg cttacattta cagcctgtgc ttccaatgat aaagacaagt
     4021 ctagtggaag caaatctggt ggaactgttg taatacctat ggcaagtgac ccagatataa
     4081 tcaatggagc ttttgcaaac gttaaagaag actctcttgc atcaaatata gtttatgctc
     4141 ctctttatac ttatgaaaaa gggaatttag taaattattt agcagaaaaa gtagatttta
     4201 aagatagtaa ggaattaact ataaaattaa aatccaattt aaaatggcat gatggaaaac
     4261 caataactgc tgaagatgtc ttatttacat ttaatacagt tttagacgaa aaacaaaatt
     4321 caccatcaag acaatactta ttggtgggtg aaaaaccagt taaagtagaa aaaattgatg
     4381 atttaacagt taagattact ttaccaactg caagtgaatc tttcttatat ggtatatcta
     4441 aaatttctcc aataccaaaa catgtatttg aaggagaatc aaatatagct aagtcagaaa
     4501 aaaataataa tccagttggt tctggtgcat tcaagtttaa ggaatggaaa aaaggtgaaa
     4561 gcatagtatt tgaaaagaat gcagattatt ttggtggaga acctaaggca gattctatag
     4621 cgctaaaaat aattcctaat gaagcatctc aagaagcagc tttaaataat ggcgaaattt
     4681 cacttatgaa gacatctgct gaaggatatg aaaaggcaaa atcaaactca aacttacaaa
     4741 cttatactta tagtgaagaa agattaaact acattgtatt taaccaaaat atatcaaaca
     4801 tggctaacaa agaagtaaga caagccttat catatgcctt aaatagaaat gaaatgatag
     4861 aatcagcata tggaaaagaa ggttctgtac ctgctaaatc tatattagtt ccagaagcag
     4921 acttctatac agaagaaggt gttgaaggat atgaccaaga tacaaataag gctaaagact
     4981 tattggataa aagtggtgtt aaaattgata aattaaaaat aggttataac acaggtagat
     5041 ttgggcataa aaactatgcc ttagtagctc aacaagagtt gaaaaaaatt ggtatagaag
     5101 ctgaaatagt tccttatgaa agtaaggcat tctttaatat actatttagt aatagcactg
     5161 aatgtgatat gtatgtaaat ggttatgctt ggggattaga accaaaccca tatagaggta
     5221 tgtttgaaac tggtcaatat tgcaaccaaa ctaaatattc taatgctgaa atagatgctt
     5281 tatgggaaaa aggatttact gagttaaaca aggaaaaacg taaagaaata tacaaacaaa
     5341 tacaacagga tatatctaag gatgcaccta tttacactat agattatgaa caaaatttaa
     5401 tggcagctca aaaaaatcta aaaggaataa aagatgctaa accatctcct gcaatattat
     5461 ttgaagattg gtctaaactg tatgtagaat aattgatgta aaattttata ttattaatta
     5521 gaaaaggaga tgtctaaaaa taatattgtt ttattatttt aaaacgagac atctctttta
     5581 attaggagaa tttacttatg ttaaaattaa tattgaatag agtaaaagtt ttaataccta
     5641 tgttaatctt aatatctata ttatcatttg gacttttaga attagctcct ggtgacccag
     5701 cagatgcata tataaatcct ttaatgtctt cacaggatat tgaaaatata agggttaata
     5761 tggggcttga taaaccagta tatataagat atttaaactg gttgaaaaac actttaagtg
     5821 gaaacttagg tatttcttat ataaaccata tgcccgttac agaacaaatt atggaaaaaa
     5881 tgggaaatac atttattctt atgggtactt ctttgatatt ttcaatattg gtagctatac
     5941 ctcttggaat atttttagct gtaaataaaa attcaataac tagtaaagta tcatctgttt
     6001 ttaattatat aggtgtttct atcccttctt tttggatagg tatgatactc atttcaattt
     6061 tttcagttaa acttaacatt ttcccatcag gaggtatgca tactattgga aatgatagta
     6121 ttggagattt agtaaaacat ttagttttgc ctgtaataac tttaggtcta tataatactg
     6181 ctatttttac aaattatgta gaagctagtg taaatgaaca attaaaaaaa caatatgtta
     6241 ttacagcaag agcaaaagga ttatcagaaa aagtcatact atttaaacat gtcttgaaaa
     6301 attcactgac atcattggtt acaatcctag gcatgagtat acaaaaatta gtcactggtg
     6361 catttgttac agaagtagtt ttctcttggc caggtatggg aagattaatg attgattcaa
     6421 ttttttcaag agattatact gtaattatgg caataactat gttatcagca ctatttttaa
     6481 ttttaggtaa tttagttgct gatatactat acttattaat tgaccctaaa ataaaatcta
     6541 gcaaaggagg attctagttg tgtttacaag tatcttaaaa aaattaaaaa aagataaatc
     6601 ctttgctttt gcatgtatta ctcttagcat aataatcata ttaattatag cttcttttat
     6661 attaaatata gactctaatg caattgatac tacttctaaa cttcaagaac ctagcttaaa
     6721 gcatatcttt ggtacagacg aacttggtag agattacttc tctagagctc tttatggtgg
     6781 aaggatttca cttagtgttg gtattttatc aatgcttatc tctgtagtat ttggtactgc
     6841 tgtaggtgtt attagtggat atattggagg taagctagac caattcttga tgagattagt
     6901 agatatgctt atgagtatac caagtttctt agttttgatt gtattaaata cttatctaac
     6961 acctaaagtt tccactatta tactaattat tggattctta agttggatgg aagtttcaag
     7021 aattgtacgt ggcgaaacat taaaaataaa ggaaaatgaa tactgtttag ctgctaaagc
     7081 tactggtatc aagactagaa atatactatt aagacatata gttttaaact taaaagagac
     7141 tattgtagtc gctggttcgt taaatattgc taatgctatc ttaactgaat ccgctcttag
     7201 ttttttaggt cttggaatcc aattacctct tgcatcatgg ggtaatatgc ttcaagatgc
     7261 tcaaagacat atttttgaca agttatattt agctgtattt ccaggattac ttatattttt
     7321 aacagtcata agtttgacta taataagcaa aaaattttct agtaatactt aacaaatttt
     7381 atatagaaaa taaaaacagt gattatctaa agatatacac tgtttttatt tttcgtttaa
     7441 aaaatacata ttttgtatat tataacttta aatactactc taccatgtta tgtaaagcct
     7501 attattgctc tcaaacatat agatgaaaaa ctaatagttt attgtaataa aaattgattt
     7561 atttatttgt tattttaata acatttttaa gagttctttt tactaaagaa atctataata
     7621 aatttaaata catgttggtc tacatggcta taacgttctt ttttaggtct acatagtata
     7681 actttccatg taataggatg attaaaaggc ttttctataa ttttgctatt ttcaaaaaaa
     7741 tcatgaactg gtgatggtaa aattgttata aaatcagaat ttgtggttga tagcaacaaa
     7801 aaatcccatg aaccagacat tatatatctt tttaacttaa cattttcatc agtaaatttt
     7861 tgcattagct tatgatgtat cataaaagta ggattaaata ttgcaagtaa ttgctcattc
     7921 aaatcgttcc aatctatctt atccttctta gcaagtggat gatttatatt taaaaatgct
     7981 gtaagttcat cttcttgaag cacatgttca gttactacac ttttatcaat tttatgtgga
     8041 tgtaacagta ctgcataatc tagttcctgc aagataagca ttctatttaa gtcatatgca
     8101 cccttctcta ctatatcaaa ttctatatct ggattatttg caactaactg tgcaactaca
     8161 tctgaaaatg ctattccaag tataagtgga ggtattccta ttctaacttt tcctttaaat
     8221 tgaactgaat cttctcttaa atcttccatc atatttttgt attcatttat aatatgttct
     8281 gcatttacat aaaatctttc tcctgctggt gttaacccat ttaacctacc tttatacctt
     8341 tcaaataaat atacatcttc actttcttca aaatttttta taatttgact taatgctggt
     8401 tgagatatgt gaagtgtctt agatgctatt gataaattaa aaccagattt cactatttca
     8461 acaaaatatt tcaactgttt tatatccatt ttaaaccccc ttccccaaaa gtataattta
     8521 gattatattt tttcttctaa tgatatttta taggaaaatc aacataattt cattaatatt
     8581 ttaacaaaat atttttctaa atctactata aatttttatc ctataaattt ttcttataca
     8641 ctatataaaa aattagactt agacaaaatg cttttttcgc cgtaatctat aattagaaac
     8701 aggaaaacct aaagcattag gaataaaagt aattgaaata cttaaaaaca agcttgtatt
     8761 aaaaaagtac tttattatag tgttccctaa tttctttatt cagaaaattt ttaattatca
     8821 ttcaagaaag gtaactatta gatatgaaag atgtagtaat cgtatcagca gtcagaacac
     8881 ctataggaag ttttggaggt gtatttaaaa atacttcagc tgttcaactt ggaactatcg
     8941 ctgttaaaga agcgatatca cgtgtaggac ttaatttatc tgaaatagat gaagtaataa
     9001 ttggaaatgt tcttcaaaca gggcttggac agaatgttgc taggcaaatt gctataaatt
     9061 caggtatacc aaattctgtt ccaagctaca cagtaaataa attatgtggt tcagggctta
     9121 aaagtgttca gctagctgcc caatcaatca catctggtga aaatgatgtg gtaattgcag
     9181 gtggtactga aaatatgagc caagctcctt atatcgttcc aactgctcgc tttggtagta
     9241 aaatgggtga cataaccatg gtagatagta tgctaacaga tggccttatt gatgctttca
     9301 atcagtatca tatgggaata actgcagaaa atattgcaac taaatttgaa tttactagag
     9361 aaatgcaaga taaattggca ttagaaagtc aaaataaggc tgagaatgct ataaaaaaca
     9421 accgctttaa agaagaaatc gttccagtag atgttttaat acgtcgagga aaaatagaaa
     9481 ctattgataa agatgaatat cctaaattag gcatgacatt tgaaggttta gctaaactta
     9541 aacctgcatt caaaaaagat ggtactgtaa cagctggaaa tgcctcaggt attaatgatg
     9601 gtgcggctat gttaatacta atgagccaac aaaaagcaga cgaactaggt attagaccac
     9661 ttgctaaaat aaaatcctat gcttcagctg gtgttgaacc tgaagtcatg ggtactggtc
     9721 caatccctgc aactagaaaa gcactgaaaa aagctggact tagcatcaat gatatagact
     9781 taatagaagc aaacgaagcg tttgctgccc aagcattagc tgttaaaaat gaacttcaaa
     9841 tagattcatc aaagttaaat gttaatggtg gtgcaatagc attaggacat ccaataggag
     9901 caagtggagc tagaatactt gttaccttaa tttatgaaat gcaaaaacgt aaagttgaaa
     9961 ctggacttgc aacactttgt attggtggtg gtcaaggaat atcaatggtg gtttcacgat
    10021 aggaggaaat atgaataaaa tagttagcat tgatgaagct ctatctcatg taaaagatgg
    10081 agatacaata atggttggtg gattcatggc caatggttca ccagaaaatt taattgatta
    10141 tttatgtagt aaaaatataa aagatttgac actaatttgc aatgatactg gctttattga
    10201 caaaggagtt ggcaaaatgg ttgtaaataa gcaatttaaa aagataattg cttctcatgt
    10261 aggattaaat aaagaaactg gaagacaaat gaacaacaat gaaacagatg ttgagttagt
    10321 tcctcaggga actcttgctg aacaaatacg tgcagctggt tatggtctag gaggtatact
    10381 cacaccgact ggtattggta ctcttgttga ggagggaaag caaaaaattg ttgtcgatag
    10441 caaagaatac cttcttgaaa aaccaattta tgcagatgtt gctcttctat ttgcatcaaa
    10501 ggtcgataaa gcaggtaatc tagtttataa aggctctatg aacaatttta ataatttaat
    10561 ggcatctgct gccaaaatta caatagttga agcagatgaa atcgttgaaa ttggtgacat
    10621 tgacccaaat gaagttaata caccaggtat ttttgtgaat tacatcgttg aaggaggtaa
    10681 tttataatgg ataaattaga aatgcaagaa tatattgcaa atagagtatc taaagaatta
    10741 aaagacggag ccgttgtaaa ccttggaata ggtctaccaa ccaaagtagc aaattatatt
    10801 ccggataatg taaacgttat cttacaatct gaaaatggct ttttaggttt aggttcagcc
    10861 gaagatggaa attcaagtga tgaaactatt gtaaatgcag gtggtcaacc agttactata
    10921 cttcctggag gatgtttctt tgatagtgct acttcttttg gaataatcag aggaggtcat
    10981 gttgatataa ctattcttgg cgcacttcag gttgacaaat atggcaatat agcaaactat
    11041 atgattccag gtaagatggt tccaggtatg ggtggagcta tggacctagt aactggagct
    11101 aaaaaagtta ttgtagcaat ggaacatact tcaaaaggtt ctgcaaaaat attaaataat
    11161 tgtactcttc cacttactgc aacaaatgct gtagatttga tagttactga aatgggagtt
    11221 atggaagtaa catcagatgg aattttactt aaagaaataa atccagcttt cacattagat
    11281 gatgttattt ctgctactga agctccttta atattgtcag attcattaaa tggaaaaata
    11341 tctgttgttt aaaataatta aaactagaaa ataaaaatct cttagacgta ttacatataa
    11401 atttgtattg cttaaaatat taatataaaa ataaaataaa attggaggaa atgaaaatgg
    11461 ttaaagataa agttgtattc gtaacaggag ctgcaagtgg aataggtaaa caaatagctg
    11521 aatcgttctt aaaaaatggt gctaaagtaa tgttctctga tataaatcaa gaagctttaa
    11581 ataataccat aacagagtta caaaaaaaag gttatgattg catgagtgta aagtgtgatg
    11641 taactaagga agaagaaatt aaccacgcta tagataaaac tgttgaaaag tatggcagat
    11701 tagatatatt aataaacaat gctggacttc aacatgtttc tatgatagaa gatttcccaa
    11761 ctgataaatt tgaatttatg ataaaaataa tgctgacagc accttttata gctactaaaa
    11821 aagcatttcc tataatgaaa aaacaaggct ttggtcgtat aataaatatg gcctcaatca
    11881 atggtgtaat aggatttgct ggaaaggctg cttataactc agcaaaacat ggacttattg
    11941 gactaacaaa agttgctgct cttgaagctg ccgattctgg tataacagtt aatgctttat
    12001 gccctggata tgtagatact ccacttgtca gaggtcagtt taatgacctt tcaaaaactc
    12061 gtaatatacc attagaaaat gtcttagagg aagttttgta cccacttgta ccacaaaaac
    12121 gcctattaga tgttcaagaa attgccgact atgctatgtt cctagcaagt gataatgcta
    12181 agggtattac aggacaagct tgcttactag atggaggata tactgcacaa tagttattat
    12241 atttagggct tgaagaaagt attcttcagg tccttctact aaggagggat atttatgagt
    12301 attatcgcac caattggtat aattattggt atcatagcta ttatatattt ttcagttaaa
    12361 gaaattcata taactatagc tgctccaata gcaactttaa tagttgttct cttaaataaa
    12421 atggatgttg taacatcaat gctaggtgct gacaaaaaca attatatggg tgcacttgga
    12481 aactatataa tgagttattt tgcaatattc ttattaggct caatacttgc taaatttatg
    12541 gaagaaagtg gagccactgt atcaatagct gattttattt taaataaagt tggctctaat
    12601 catccttata gagttttagt tgctatattt atagtaagtt ttatattaac gtatggtggt
    12661 ataagcttat ttgtagttat gtttgcagtc attccacttg caagaactct atttaaaaaa
    12721 cttgatattt cttggaacct tattcagata ccactttggc ttggtattgc aacaattact
    12781 atgactataa ttcctggaac accagccatt caaaatgtta tacctattca atatctaaat
    12841 acgtctctta ctgcagctac tataccaagt attgtaggta gtataggttg tgctacgttt
    12901 ggacttgtct atatgaaata tgctctaaac aaaagtatta gaaaaggtga aacttacgca
    12961 acttacactt caaaattaga agaagaatct ttagatagag aattaccagg attttttgca
    13021 agtatttttc ctctattagc actcgtaatt atagctataa ctggaagtac ttttggttca
    13081 gaatttatga aaaaaaatgt aatttacata gctttaatag ttgcaattct tttatctgct
    13141 tttttattcc ataaatttct atcatctaag atacagacat tgagcatagg tgctagtggt
    13201 tctataggtc ctatatttgc tacatcttct gcagttgcat ttggagcagt aataatggct
    13261 gcacctggtt ttgatgtatt tagcaaactt ataatgtcaa taccaggtag tccacttata
    13321 agtcttactg ttttgacttc aacaatgtca gcaataacag ggtcctcttc tggtgcatta
    13381 ggaatagtaa tgccaaactt tgcagattat tatttaagta ctggtctaaa tccagaatta
    13441 attcaccgtg ttgctacaat agcctcaaat atactaacta tagttccaca aagtggtgtt
    13501 tttcttacat tcttaagctt atctaagtta acacataaaa ctggatttaa agaaactttc
    13561 attgtagttg cagttggttc actaatagca gaaattattg taattacatt aggattacta
    13621 atgtattaat ataaaacaaa ttaaaattca taatatgaaa ggtggtgtct caaagtgaac
    13681 tttttactta atgagacact ctttttgtat gaaatcaaat atattttaat catttcaaca
    13741 ataaaaaaga acctttctct attttgagac aaaccctttt tattccttaa agatttaaac
    13801 aattataatt tgttactaat acgaaaacat ctattgaata aatttaacca aataaaaaaa
    13861 aatttatcat aactataaat ttacgttatg gaattattat aaaaatatta ttttctctaa
    13921 gtaagtaatt ccatactata caggctttta atacaaattt tcaaacacac tgaaatatac
    13981 ctatttattc catatatatc ttttttacat atctttacag taaaaaaagt cttaatctta
    14041 tgttagtata gattcacggg taccccctac actaaaggtt acaaatcagt aacattttta
    14101 gaatttttag attaagaggt gtttaatgat gagaagaaat ataccaacaa aattaaaaac
    14161 tatattagct ttaggagtgt taactttagc aacagcttca agtgctgtta ctgcaagtgc
    14221 ttcatcactt gaaaataatg taaatataag taacaaaagt ttagtatata gtaatactaa
    14281 atacaagttt tctgcaaaac caaattgttc tgtaaaacca gaacttaaac ctgataaaga
    14341 taactcttgt aaggataaga atcataatga caagaattgc aacaacacta ataaacctga
    14401 ggacaacaat aattcagact ctactaataa gcctgataac aataaaccag agtctaataa
    14461 acctgaggac aacaataatt caggctctac taataaacct gataacaata aaccagagtc
    14521 taataaacct gaagacaaca ataactcagg ctctactgat aaacctaata acaataaacc
    14581 agagtctaat aaacctgaag acaacaataa ttcagactct actaacaaac ctgatgataa
    14641 taacaactca ggctctacaa gtgaaaattt ttcagcttac caaaaagaag ttgtcgactt
    14701 agtaaatata gaaagagcta aagctggatt aaatcctctt actcttgatt cttctatatc
    14761 aaatgttgct acaaaaaaat ctcaagatat gattgataac aactattttt cacacaattc
    14821 tccaacttat ggttctccat ttgacatgtt gaaaaaattt ggaataagct ataagacagc
    14881 aggtgaaaat atagctatgg gtcaaaaaac tcctaaagaa gttgtaaacg catggatgaa
    14941 ttctgaaggt cacagaaaaa atataatgaa tccaaatttc agtaaaatag gtgttggagt
    15001 agcacaaaaa agtggtggtt caatttattg gacacaaatc tttgtaggat aaaatactct
    15061 ttttccatgc atatcaaaag agtatctcta agtgataaaa aacacaaatg agatactctt
    15121 ttgtatctat tttaaaatta tcaaatccaa atatacatac tataaatatt atttgttctc
    15181 ttaattttag tgtgtagaac aatatataaa aagtattggc aagatgaaaa tggaaaactt
    15241 aaaaagatat ttaataggta aaaacaggat cttaatatct tcataaaaaa aggctgtctc
    15301 aaaatagaga taaggccttt ttcattgttg aaatgattaa aatatatttg atttcataca
    15361 aaaagagtgc ctcatgaact aaaaagctca ctttgagaca ctctctttta tcaattctat
    15421 ataagcatta taatacttta atattattca gctaattttt tgtatccttc atatctttct
    15481 ttagcatcag cttcagcttt agcaaataat ccatcagctt cctctgggaa ttgtttagct
    15541 atagcagaat atcttacttg tgcaagtatg aaatctttga agcttgcagt tggttctttt
    15601 gaatctaaag tgaatgggtt tttaccttca gcttttactt ctggattgaa tctgtataaa
    15661 tgccagtatc cgcaagcaac agcttgtgct tcattagcca cagttcttcc cataccttct
    15721 tttaatccat ggtttataca tggagcataa gctattatta gagatggccc atcatgtttt
    15781 tcagcttcta aaacggcttt gataaattga tttttatcag cccccatagc aacttgagct
    15841 acatatacgt ttccatatgt catagccatc attcctaaat ctttctttct agatttttta
    15901 cctgaagcag caaacttagc catagcagca gctggagtag ctttagaagc ttgacctcct
    15961 gtatttgaat atacttctgt atcaaataca agtacgttta catcttcacc agatgcaaga
    16021 acatggtcta atccaccgta accaatatca tatgcccaac cgtctccacc tagtatccat
    16081 tgagatctct taactagata atcttttcta tcttttatag cttttactaa ttctttagct
    16141 ttttcatctg tacaatcaca agctggtttt tctaataatt caacaacctt agcacttgct
    16201 actttagaag cttctccatc atttctagaa tctaaccact ctgtaaatac tgctttagca
    16261 tcttcacaga tatccataga aactaactct gtcatagcat ctactatttt atttcttatt
    16321 tgcttaacag ctgtgtacat accaaatcca tattcagcat tatcctcgaa taaagaattt
    16381 gcccaagatg gacctttacc ttcatggtta caagtataag gaattgatgg tgcagaagct
    16441 ccccatatag aagaacatcc agtagcatta gctatcatca ttctatctcc atataattgt
    16501 gtaactactt taatgtacgc agtttctcca catccagcac atgctcctga aaactccatt
    16561 aatggttgtc taaattgact tcctttaaca gtatttgcag gcataacatc aacttttgga
    16621 gctacttcat caggatttac tgcaaatgcc caattatcta actctgtatc aagttgagta
    16681 tctattggct tcataactaa agctttttct ttagctggac atatgtcagc acagtttcca
    16741 catcctgtac agtcaagtgg actaacttga attctatatt ttaatccttc taatccttta
    16801 cctaatgctt ttttagcttc aaatccttct ggagcattag ctaattcttc ttcagtaact
    16861 aatactggtc ttatacatgc atgaggacat ataaaagaac attggttaca ttgaatacag
    16921 ttatctatta tccattcagg aacatcaaca gctataccac gtttttcata agctgcagta
    16981 ccacaaggga atgttccatc ttctatacca ttgaatgtac ttactggtaa gttgtttcct
    17041 tcttgtgcag tcattggtct tagtatattt tttataaatt ctggttcttt aacttctcct
    17101 ttatctgcat caacagcatt ttcccaagaa gctggaactt caactttaac taaagcattt
    17161 attcctgcat caacagcagc atagttcata ttaactattt tttcaccttt tttaccataa
    17221 gctttaacta tagaatcttt taagtattta acagcatctt cttctggtat tatttcagct
    17281 aatttaaaga atgcagattg cattatcatg tttattctgt ttccaagacc tatttcttgt
    17341 cctagtttaa cagcattaac tgtatagaat tttatatcat ttttagctat atattgtttc
    17401 atatgagctg gtaaatgttc ttcaagtcct gcttggtccc atatagtgtt aagtacaaat
    17461 gtaccacctt ttttaagacc ttttaataaa tcatattgat aaacatatga ttgtttatga
    17521 caggcaatat aatcagcttc atctattaaa taagtagctc ttattggagt atcaccaaat
    17581 cttaagtgag acatagttat accaccagat tttttagagt cataatcaaa gtaagcttga
    17641 gcatattttt cagtatggtc accgattatt ttgatagctt gtttattagc tccaacagta
    17701 ccgtcagaac ctaatcccca gaacttacat cttatagttc ctttagaagc tatttttatt
    17761 ggctcagatg gagttaaaga agtattagtt acatcatcaa ctatacctac tgtaaatcca
    17821 tttttaggtt gttctgaaac taagttatca aaaacagttt ttatatctga aggagtagta
    17881 tcttttgaac ctaatccgta tcttccacct attatcatag gagcattttc ttttccatag
    17941 aatacatcac gtacatctaa gtataatggt tcaccagttg aacctggctc tttagttcta
    18001 tcaagtacac atattctttc aacagtagat ggcatagcat ctaagaaatg tttcattgag
    18061 aatggtctat ataaatgaac tttaactaaa ccatattttt ctcccttagc atttaagtaa
    18121 tctatagttt cttctattgt ttctgttact gaacccatag ctattaatac gtatttagca
    18181 tcttctgctc catagtaatc aaataaacta tgttttctac ctgttaaatt actcattttt
    18241 tccatgtatt tttcaactat acctactata ttttgatagt atttattaga agcttctcta
    18301 gtttggaaat atatatctgg gttttgagca gtaccacgag caactggatg atttggagat
    18361 aaagcattat ctctaaatgc ttgaactgct tcaaagttta ataaacttgc ataatcttca
    18421 ttttctagta attcaacttt ttggatttca tgtgaagttc tgaatccatc aaagaaatgt
    18481 ataaatggta atctaccttc tattgcagct aaatgtgcaa caggtgctat atcagctact
    18541 tcttgaactg aaccagaagc taataataca catccagttt gtctagcagc cataacgtct
    18601 tggtgatccc caaatattga taacgcttga gatgccaaag cacgagcact tacatggaat
    18661 actcctggta ataactctcc tgcaacttta tacatgttag gtatcattaa taataaacct
    18721 tgagatgctg taaaagtaga agttaaagct cctccttgta aagaaccgtg gaatgttcct
    18781 gatgcacctg cttctgactg catttcaaca acattaactg tttgtccaaa tatatttttt
    18841 cttccttgtg atgcccactc atcaactact tcagccatag ttgaagatgg tgtgattgga
    18901 tatatagctg ctacatctgt aaacgcatag gcaacatgag ctgcagctgt atttccatca
    18961 agtgttttca taaacttagc cataatatgt atcctccttg atagctacaa ttgtacttgt
    19021 ttataacaag tctatttttt tatatttaca tataatttac acaacaaaaa tataaacaca
    19081 catatagtcg cgcaaatact ctatcttcta atataatact acagattaga taaaattcct
    19141 ttgaaaattg taaattaatt acatatttaa gtagattttt tttaaaatta aatatgttaa
    19201 gtttttttca taattttgta tacaatatat aataaattaa gttagacttt tcaaactttt
    19261 ttatctattt gtttacatta gtataaaaag taaaataatt atctttatta tatgtaaatt
    19321 tttatatata taattttacc atattacatc atatttttgt aagtttttat tacatttttt
    19381 ataatacccc tttattttat aaattaatct aatcgattaa atttatccta tagaaattta
    19441 ttacatttaa aaatatgcta gcaaataaaa tatctactag cataaagtct atttataata
    19501 ttaatcaact attaattcta ctaaatctcc atttttaacg cctgctgcat ttccctcttc
    19561 catatcaaca tgcatttcta gattaaagct atcactagct cttactaata cattttcaaa
    19621 tactaatgct cttggtccag atgttttaac ttttactatt tgcttatcag ttacaccaaa
    19681 tttagcagca tcttcattgc tcatatgtat atgtctagaa gcaactataa ctccttcttt
    19741 catttcaact tctccagcag gtcctactaa tttaacacct ggagttccag ctatatcacc
    19801 agattctttt attggtgctt ttactcctaa tgggaaacca tctgctaaag atatttcaac
    19861 ttgagtatga gctctagctg gtcctagtac tctaactccc tttaaagttc cttttggtcc
    19921 tactaaatcc actttttctt cacaggcata ttgccctggt tgagataatg gtttaaattt
    19981 agttaactca tgacccgccc caaataatac gtctatatca gcttgactca aatgaacatg
    20041 tttattagat aatgctattg gtaatttcat tgaaattccc tccttttaat tatttaaatt
    20101 attgcaatac aattatacat aatattacct acaaaatcaa attatattga atcaatatat
    20161 atctttaaat ttttatatat tatatcttta aattcttgat tatctatata agatacatca
    20221 tctgtattgt ttaaatatat attgtgtaaa agtttagtat ttatactctt aaagaaaaaa
    20281 tccataacaa tctgtccacc tttaaactgt gaatcatatg atttactccc ccccactgca
    20341 atatacattc ctatccgttt cttatttcta tcaattgaag gtttatttaa tacatattta
    20401 cttgcatata tagcttgagt tctatcaaca acagtcttta atttagctga tattgaatca
    20461 aaatgtacag gacttattac tatagttcct tttgaatcat caaacatttt atataatggc
    20521 ttcatatcat cattaaactt gcaataccct gttttttcac aaaatccaca tgctgtgcaa
    20581 tattcaatat ccatattgta gatattaaat attttacaac ttaacccaca ctcttctagc
    20641 atagctttta ctttatttgc tataaaactg caatttttat taattcttgg acttccattt
    20701 attattataa tctctgtact tttaagtcct tttttatcgt ttgaatccat aatcaatcta
    20761 cctatccttt aacataacta ttaaaatatc atcatcataa ttatcttttg atgagaaatt
    20821 ctttaaatcc aattttagat tttctactat taactgttga tttaatctaa aattttcttt
    20881 tagaaagttt tctaatctgt ttataccata ctcttctttt gatgaatttt ttatctctaa
    20941 aattccatca gtgtacatac ataccattgc ataatcttcc attgaaagag tattattttc
    21001 ataagtagca tctttcatta ttccaatagg tatgcccttt ttacaattta aattattttc
    21061 tattgtgcca tctttcttta caactacagg actatagtga cctgcattag acacagtaat
    21121 catattttct ttagtatcta taatacctat caaacaagtt gtaaatactc ccatcttgtc
    21181 aaattcatca tataacatcc tatttaaatt tgtcattatt tctcttggtg tattgtattg
    21241 attgcataat actttaaaag caccttttat cattgcaaca acataatttg ataccattcc
    21301 atgtcccatt acatcagcaa caatataaac tatatgttcg tcatccactt ttgtagcata
    21361 ataaaaatct ccacctacta cccttgctgg tttatggtaa aaataaagtt ctttctcatc
    21421 attacaatcc attttacctt tatccataat aagctgttgt tgtttattta atatgttcaa
    21481 ctcattttca agaactttgt gttttacttc cttttcatta tgttcatata attgcatccc
    21541 aacagaaact tgtttggaca atatactaag aatatttaaa tcatcactag tatagttttt
    21601 actatttgca caaactatac atcctatagt ctcattttta tcctgaagtt tgtaatatat
    21661 ataactttcc aaatttatgc ccttttttga gcatggtttt aaattattta ggtatccccc
    21721 taatgactct ttctttaaaa acatatcaat cataggatat aaatttgttt tctttttttg
    21781 ctcaacatta taacaactaa ctatagtatc cttattatga tgactattta taattattaa
    21841 agcttcttta ctattagtaa tattacacgc ttgtttactt aaattttcca aaaagccatc
    21901 aatactctta ttaaggtata gttttttagt agcttcattt atggcactta ttgagcctct
    21961 tagtttatca attaaaattt gtacattatt atttttatta gttatttcta ttgataaact
    22021 taagagtgat gcaacagatg atataaaatc tatatcttgt tctgttatat aatcatcctc
    22081 ttttaaaaaa caagtcataa atccaacaac tacattattt attataaaag gaaaaactat
    22141 tctcccaata taaccttcag cttttgctaa atctcgttca ccttctgctc tagaatcttc
    22201 aaatacattt tccacatata ttatcttttt ttcatttaca gcctcatgta tatactctgg
    22261 atatgtgtca aaatcaattt ttgcaccttt aagactattt attggaaaaa tttgaggtac
    22321 atattctaag gtttgcgaac atactaagta ggcatattta gaatcatttt tataaaatag
    22381 atttacacat gcctttgtag ggtgaacaac ttctaacatt ttttctatta tgtcatcttt
    22441 tatattaaaa aaatctgtag attccgtcac catagcggat atctccacta aattcttcat
    22501 tttataactg ccattagtca tactcaattt ctccccctat atattaaact atttattagt
    22561 attttaacat acattcttta tagtaaaaac aaaaacaacg acaaaactag caactaatat
    22621 ccagttaaat cctgtcaata atcctataag aagtaatgta atcaatgcat ttagacctgt
    22681 tctccctttt tcatccataa aagttatttt agagatgtcg atattatttt tcacattatt
    22741 tatatgctct tttacttgtt cttccatatt tcttttatat agtatataaa ctacaccaat
    22801 taaaaacact cctataccta gaatcatgtt taaattaaaa gctatcataa gcagtattga
    22861 accaaaagcc aactttaaaa gcttaaaaaa tacgctatct atctttagag ttatatttct
    22921 gatttcttta cattcatctc ttaaagaata tccacttgag tctaggttat taatctttgt
    22981 ttttacaatt tcagaataaa aaagctttat tatcccattt aacattttca tcactcctaa
    23041 atattttaca tatatttgta agttttacaa ttataatatt ttttatactt ggtaataata
    23101 aaaaataaca aatattttat gatttagtat ttactgtaaa taaattattg aagctttatt
    23161 agttaagtat agatttttaa taaatatttt tataattaga aggaagtggt tttgtgcaaa
    23221 tagaacaact cgatttagaa acgagaaata aaatatactg ctatactaaa aaaattctta
    23281 gaaaatatca aaaaggcata acatctggaa aattaacagc agataaattt gcagataata
    23341 tattgtctca acattttatc agtagtatac ttaatgaaaa aatagtcaat gagacaaatt
    23401 ttaagatatc ttatagaaac tatatagaaa ctttaataaa tattcaaaat gaaaatttat
    23461 ctaacttaag aaaaaaatct actaaaactg caaaatgttt caatatctct caaataaccc
    23521 aattaaaaaa cttactaagt aatactggtt ataatttatt aattccatat aaatacttaa
    23581 ctgctagaga tatagaagga atcgttactc ttattaatac tgggtctatt gaacttggaa
    23641 atgagagaat ctataattat atatcaaaag cataataatt taaacctatt ataaaaaggc
    23701 aatttattta ataaaaaata tttttaatat taaataaatt gcctttttaa ttttggacta
    23761 aatactgcta tccaagtgct ttttctagtt tagataaatg ttgtattgga taatatttta
    23821 gtagctcttt aaattgttct actgataaac cttctgttgt tgtatatatt cttatttttt
    23881 catctaagtc actattaata aatttgtttt ttaaatcctc aaaatttaca ctactcattt
    23941 tacatcactc cttatgtaac atattcattt gtattatttt atccaaatac ataaaattta
    24001 ttacataaaa atatatctat ttaataattt aataaaaaaa agctatctca atttagaaat
    24061 tactttctat atttgagata gctctagcta aactaatagc caatagttat aaataattat
    24121 ttataactat ctgttgcttt ttccagccat ttgttgttca gccatttcaa ctaatttttt
    24181 agtcatgtat ccacctacat aaccattttc tcttgcagta aggtttcctt tatcaacttg
    24241 ttgataatta ctcataccaa gttcattagc tatttctaat ttcatttggt ttaaagcctc
    24301 ttttgctcct ggtactactg ttctgttgtt gttattgtta cttgccatgt tgattacctt
    24361 ccttctgttt aatttatttt gtgttgctga taatattatg tgattttaaa catttttaat
    24421 tcaggtaatt tttagcaatt aaaacctgcc aaatatccag tagaaaaagc tatttgcaaa
    24481 ttgaatccac ctgtatatgc atctatatct attacttctc cagcaaaaaa taaaccttct
    24541 actaattttg attccattgt gcttgaattt atttcgttta cttttacacc accagaagtt
    24601 attatagctt cttcaatagg tctgtatctc tttactgtaa actttaaatt ttttaataaa
    24661 tggactaagt tttttctctg ctctctagaa atttggtgaa caaccgtatt tgcattaatt
    24721 tcacttaagt ttattattat aggtattagt ttttttggaa gtaaatcatc taatgctttt
    24781 tcaaattttt tatttgtata cttctgaaaa tctttttgaa ctcttttatc cagtttttcc
    24841 tcatctaatg caggttttaa atctaataat atagtgtaat tttctttcct tgtatctttc
    24901 attctacaac ttgcagactt gattataggt ccatctaaac caaatctagt aaattcaagt
    24961 tctccaaaat cactataaac tttcttttgt ttgctattaa acacccttat ttcaacattt
    25021 ctcaatgata atttttctaa atcttttgta aaactttctt gtacttctat accaattaaa
    25081 gatggttttg tatctataat tgtgtgcccc tgtgaaattg caaatttata tccatcacca
    25141 gtagaaccag taagtggata acttaatcca ccagttgcta ctacaactga atcacactta
    25201 atttcttttt tatcgtttaa aattactttc tcaattttat tatttttaga tattattttt
    25261 tctacttttg aatttaataa tatatttact ttcttacttt taagttgtct ttcaagagca
    25321 tttacaatat caaaggcttt atcactctct ggaaaaaccc tttttcctct ttctgtttta
    25381 gtctttactc caagattatt aaacatactt attacatcat catttgtaaa agtataaaaa
    25441 gcactataaa gaaattttcc atttgtaggt acattttcta ttaattcctc tatttcacaa
    25501 gcgttagtaa tattacatct accttttcct gttattgcaa gtttttttcc taatttatga
    25561 tttttctcaa ttaatgttac atcatagcct ttctcacatg cagttgatgc ggctatcatc
    25621 cctgctggtc caccacctat tattactacc ttcttcaaat aatcactctt ttctaccctt
    25681 aattttttta atttaaacta ttgactctat accttaaaat caatttaatt ttaaaagttt
    25741 aaataatgct ggagctcctg ctattccata aaaaacaact actgtattct taataaaatt
    25801 cataaaaatt atactcatag tagagtcact tatatttaaa gtgactatta acattgaaat
    25861 aaacaactta atgccaatat ataacattcc tattgttact atcccaacca aaaacctaca
    25921 tattcttgaa aaaattatat ttttactccc cttattttta ttgctaaaat ttattttttt
    25981 gttattatta tctgtattaa atttaacaaa tctatcctca accatatatc caaatacaaa
    26041 tcctactatt ataccaaatt tttcaaaata ttcagggcta tttagaaata tagctactat
    26101 tataaatggt ataagtaaaa ctacaagtaa aatataattt ttattatcat caatatagtc
    26161 aaatattttt ataaatataa ctgtaaaaaa tattcctaac atccaaccca ctatcacatc
    26221 tattggccaa tgaacagcta aatacagcct agacaatcct actcctaaaa ccataactgc
    26281 acctattaaa tgaagatatc tctttttaaa tatagtcata atactaaccc aaaatgttgt
    26341 tgcagtttga gtatgagcac ttggaaaaga ataaccacca gcagtagaaa ctctcaatga
    26401 ttcaagacct tttattccta ttggtctggg agcttttaca aactctttga tgcctaaatt
    26461 tacaacaaaa tttccaatta tagcaaacag tattttctgt ccacattttt tatttataca
    26521 ccagtatata attgttgtaa ttaatattaa taacggtgct tctgtgctta ttgtaaagac
    26581 aagaaatata aagttcaaaa ttggatttct taaattttgg aaaaataata atatatttaa
    26641 ctgaatatcc atatagatgt ctcctttact aaaattttat ataattaact aaaaagcact
    26701 aactccctta gaaattagtg cttttgattc acatttaaat ctattctaca tcctcaacta
    26761 tacctatata aggtaaattt ctatactttt cagcatagtc tattccataa ccaacaataa
    26821 atttgtcttc tataacaaat ccaacatagt ctacaggtat attagctttt cttctctgag
    26881 gtttgtcaag tagagtacat agttttaaag attttggatt tctagttttt aaagctgcaa
    26941 ctagattact taatgttaaa ccagaatcta ttatatcctc tacaatcaac acattctttc
    27001 cttcaatatc tacatctaaa tcttttaata ttttaactgt tccagaagac tctgttgagt
    27061 ttccataact tgtaacactc ataaaatcta tatttacatc caaatctata catcttatta
    27121 aatctgaaac aaatacacta gctcctttaa gtatacctac cactagtaaa tcttcgcctt
    27181 taaagtcttc ttctatcttt tttcctaatt cataaacttt ttcttttatt tgttcttctg
    27241 tcaacatttt tcctgtaact ttgtacattt tgtatcctcc cgctttcctt ctattaactt
    27301 atatattata gcataccaaa atatcaatta aaatcttttt catcattttt agacctaaat
    27361 taacagctta tccatacttt tttgcttttt ccattaaaaa tttggaataa cgattccttt
    27421 gtattataat ctatatgtac atatattaat cagttatttt tatcgattat ataattttat
    27481 ttttggaggt acttttatga gcacaaatca tggagctaat ttatatagtt tatctagtaa
    27541 gtatggcttt tcaaaggaag aatttatgga ttttagctca aatataaatc cttttggtac
    27601 atcaagttta gccaaacaat acattgtaaa caatatagat atggtatcta tgtatcctga
    27661 ccctgattac atagacttaa aaacttctat atccaattac tgcaaatgtt ctatagataa
    27721 tatagtactt ggcagtggag ctacagaact aatttcatca tttatacata caattaatcc
    27781 taaacaggct cttttactct ctccagccta ttcagagtat gaaaaagagt tatctaaaat
    27841 taattgttct atagaaaaat actttgcaaa agaagaagat aattttcata taaatttaga
    27901 aaatcttata aaaactatca atgctaaaga ttatgatttg gtagtaatct gtaatcctag
    27961 taatccaact ggatttgcat ttactaaagt agaagtgaga gaaattttaa aaaatacaga
    28021 ctcattttta atgattgatg agacttatgt agagtttact gatacagata catactcttg
    28081 tactcaatta gttgatgatt attctaattt attcgttata agaggtacat caaaattttt
    28141 ttcaacgcct ggaataaggc ttggatatgg tcttatatct aacactaatg tcaaaaatga
    28201 aataaataaa aatctagatt tatggaatat aaacataata gcttctaaaa tgggggaaat
    28261 catgtttagt gatttagatt ttatatcaaa tactatttcc ttaatgaaca ctgaaagaga
    28321 ttacctgctt aaagagctta agaatataaa aagcttagat atatacaata ctaaaggtaa
    28381 tttcatattg tgtaaaatca aaacaaagga attaactgct aaatcgcttc gagaacaact
    28441 gcttccacaa aaaataataa taagagattg ttgttcattt gaaggtctag atgagtattt
    28501 ctttagagta tgtatattaa aacctaatga aaataaatta ttaatatcat ctcttaaagc
    28561 aatttttaaa taaaaaaatc cctttatttt ataatttttt tataccataa aaaatgagct
    28621 atctctattt gagataactc atttttgatt attaaaaaac taagcacttt gctcttcttt
    28681 gtttcctttt ttactcattc catcatttat tttatctact actactgcta ttgcattatc
    28741 tcctgataca ttacatgctg taccaaaact gtcttgagtt aagtataaag ctatcattaa
    28801 acttgcaagc ccaccatctg atggtattcc aaccattggt aggaaaggaa gtgcactcat
    28861 tatagcccca cctggtgctc ctggtgctgc aaccattgct atacctaaca ttgcaatata
    28921 agggaataat acacctattc catgtggcat accattcatc attaaaactg cagttgtaaa
    28981 tcctgttatt gatatcatac tacctgataa atgtattgtt gcacaaagag gtacaacaaa
    29041 ttctcttatt tgcttactta ctccattttt ttctgcacaa ccaacattaa ctggtatagt
    29101 tgctgctgaa gattgagttc ctatagctgt taaatatcca ggtacttgat tttttaacat
    29161 tcttactgga ttttttcctg aaaatacacc tgctgctgtg aattgacatg ccatatatac
    29221 aaaatgtaaa ggtattacta ctaagtatac tttccagaat acccctaata ttgtccatac
    29281 ttcccctgct atagttatat ttgcaaaagt tcctgctatg tataaaggta ataaaggtat
    29341 aatcactgtt gacaacgttt tcgttacgat tccttgaaat tcttggaaga aattaaataa
    29401 agtctctcct tttccatgat ttcttaaagc tgatattcca agtcccatta caaatgcaaa
    29461 tacgattgca gctgttacat ctaccatagg ctctaatgga attgtgaata ttggagctaa
    29521 cattcctgcc tctgggtctc caatttgact tgcaacactt gcatcaataa aatttgggaa
    29581 gattaatgta gatactgcaa atgataataa tcctgctatt aaggtagaac catatgctat
    29641 tcccgtagtt atccccaata actttcctgc acctgttgct aaatcagcta tacctgctgt
    29701 tacaaaacct ataattataa atggtataac aaattttagg aatgctgaga ataaggtact
    29761 aactgttact aatatttgta ctattatttc tggtgcataa cttccgatta aaataccgac
    29821 tatgataccg atgattagtt tgggcactaa acctattttt ttcttcataa tatttaccct
    29881 ccataacttt tttaaattaa gcattcttgc acattgaata cttagttaaa tattggatat
    29941 ccacttatta tgataataga gttttacctc tccgtcaaca atttaaccat tttttgttaa
    30001 taaaaaaaag aataacatat ataaattgtt attcttcttc atcttttata gaaaattcta
    30061 cattttcaat tatatttgaa tgtttatctt tttttataat aactctatac ttattaattt
    30121 cttcattcac tatacctata ctccaaatta aaactatagc atcatctata aaaccaaatg
    30181 cccctgcaat catctctgga attatatcaa ccccagatac aaagtaggat atagtaaaaa
    30241 ttaatacaga aattaccttt attttatcaa ctatattgat tcttttatct gtcataaaat
    30301 caggtagttt aagtaaatca aatacaaaaa ccaaaccaaa tttagcattt ttaaatctga
    30361 ccaaaatttt ttttaaaata tctaaaacta tattaatgat tatttttaaa aactccatat
    30421 ttatcacctc ttataattat aggttattct acattataac cgatataatt aatttgatga
    30481 acatattaga tataaatttt atcaattagt ttatatttaa attataataa ttcaattcct
    30541 gcactactac aattttgaat catatcctgt ggccatactc caacttgtac ttctcctata
    30601 tgagcttttt gtaagaaata catacatatt cttgactgac caattcctcc accaattgta
    30661 tacggaagtt cgccatttaa aagcatctta tgatagtcaa actcttttct ctcttcacat
    30721 ccagcaagtt tcaattgtct atcaagagat tcttcatcaa ctctaattcc cattgaagat
    30781 aattctaatg ctgaatctaa aacaggattg taaaataata tatccccatt caattcccaa
    30841 tcatcataat cagggcttct tccatcatgt ttttctcctg atattaatgt ttttcctatt
    30901 tgagttataa atactgcttt cttctctttt acaattttat cttctctttc cttcggtgat
    30961 aattctggat acatatcttc taactcttgt gatgttataa aagttatttc ttctggtaat
    31021 atttctttta taccttcata catactacat acaaaaattt cagtatcttt aaatactcca
    31081 tacaatcttt ttacaacatc ttttagagtc ttttcatttc tactactttt atctataacc
    31141 aattcccaat cccattggtc tacataaaga gaatgtatat tgtctaattc ttcatttttt
    31201 cttattgcat tcatatctgt atataaacca ctaccaactt caaacccata ttttttaagg
    31261 gctaatcttt tccattttgc aagtgattga acaatttctg catttttatt cataaatggt
    31321 atatcaaagc ttacagcctt ttctccattt aaattatcat ttgcacctgt ttctggcaat
    31381 acaaataacg gagaagaaac tcttataaga tttaattcat tacttagtct catttcaaaa
    31441 aaatctttta atttttttat agctatttgt gtatcaatta cgttcaaact acttttataa
    31501 tcctttggta ttattgtgct catatcattc actccctata tttatatatt tttccattaa
    31561 aaaaaccctt catccctata taaaagggac gaaaggttgc ttttccgcgg taccaccctg
    31621 attagtttga aataaattca aacccagctc acaatgtaca gatattactc gatacacccc
    31681 aatatgataa cggtttggtt ccgactaagc ctactctttt aaaatttcgg ttagcaactc
    31741 caggatgttc ttcattatag acttcatatt ggtctcacac catcaccaac tcgctttaat
    31801 tacattctat aactactctt cctttcaaag tctttaagag tttaattcaa taaaatttag
    31861 ctctcttatg ttttctattt attcataata gtatgataag tgattagtta aaatttgtca
    31921 agtttattat ttttttattt ttaaacactt tataaatttt tacctatact ttataaattt
    31981 tcaaatatag tctttaatct gtccatacat tcttttaata aagactgtgg acaagctaca
    32041 tttattcttt caaatcctat tccttctttt ccaaatatat aaccttcatc aaataataca
    32101 ttagcttttt taaacattaa atcctcaagt tctacttcat ttaatccata agcagtaaaa
    32161 tctaaccatg ctagatatgt tccttctgga taaacaagtt ttacttttgg taggttttct
    32221 tttaagtatt catcaataaa ttgtatatta ttatctaagt aatcatttaa ttcattaagc
    32281 cagtcttcac tttcattata tgctgctatt gtagctacaa tagcaaaggg atttggtgaa
    32341 gttgcaactc caacagctgt aacctcttct tgatatttct tttttagttc atcattattc
    32401 actattatat tagacaactg cattcctgcc agattaaatg ttttacttgg agctatacat
    32461 gtaattattt tatccttgta ctctgggcat aatgactgta aaggagtatg tttaaatcct
    32521 tttctaacta aatcagaatg tatctcatca gctattatcc ataggtcata ttttttacat
    32581 atttctacaa ccttctttaa ttcatcttct ctccaaactc ttccaacagg gttgtgagga
    32641 ctacaaagta tcagtacttt attgttgcta cacttagctt tttcttctaa atcatcataa
    32701 tccatcctat aagtaccatt ctcatatatt agaggattgt ttacaatgtc cctattattg
    32761 ctctttatct tagcttcaaa tgggtaatac actggttttt ggactattac accttcatta
    32821 gaattagtta atattcttac taaaatagca acagcaggta caacaccagg actaaaaata
    32881 atatcttttc tatttatttc ccagttaaat ctccttttat accaatcaca aactgcatta
    32941 aagtatttat cactatcatt tgaactatat ccaaatattt tatgttcaac ttttttacgt
    33001 aatgcttcta ttattggttc tgcacattca aaatccatat cagcaatcca aaaaggcaaa
    33061 ccatcttttt gtgcttttgt tgaacaatta gttctaaaat cccattttac acaatctgtt
    33121 cctactctat ctattactct gtcaaaatta tacttactca ttttttccct cctaagaata
    33181 aaatttttat aattgttaag tccattacta gatgcacttt ggtattattt tacaattctt
    33241 aatattataa taatatcact tttttgtcca atagatttaa aatataatta cttttccatc
    33301 atattttttc aatattccaa aatcagtttc attatatcaa aataagattg ctcatattaa
    33361 ttattttcta attaacataa gcaatctttt tctataactt ctataattat aattttaaat
    33421 gtagaattat tagtttggat attatcccaa tctcttttca accaatctac accatatatc
    33481 tacaccatat tctaaagctt tcgtatcaaa tgttacattt ggattatgaa atcctggtgt
    33541 agcatcagct cctaatccaa tatatgtagt tttacatttt agtttttgtg tataataatg
    33601 gaaatcttct ccaccagaat ttttaaatat acctatagat gcatcagctc ccaatacatc
    33661 ttttatagtt tcttcagcca agtttacaag ttcttcatca aattctgctg ctggtactcc
    33721 atctttggaa attagtacag cttctgcatt tatagaactt acagcagttt ttacagcatg
    33781 tattgctttt tcttttaatt cttctattat atcattactt tctgccctta tatctaaagc
    33841 cataaatgct tcatctggta tattattata agtttctccc ttaactgata tatttgttac
    33901 ttttatagaa tttggtatag ctggatttac tcttattgca tttattgcat tcactgctaa
    33961 acagacagca tctactgcat ttactcctaa atgtggttta gaaccatgac aagtgatacc
    34021 tttaatcgta aattgcatta tacagctagc acaatggtac attgcaggta tcgcctttcc
    34081 caacctacaa tcttgtccag gtcttaaatg catccctacc atttcttcta catcatccaa
    34141 caaaccagaa tcaattactc ttaatgctcc atataatttt tcttcacctg gttgaaaaag
    34201 tatttttaac ttacctcttt ttactaaatt tctctctttt attaactttg cagctgttaa
    34261 aagcatagaa gtatgaccat catgaccaca agcatgtatc attttctttt ctccatctat
    34321 gataaattcc aatgcatcca tatctgctct tatagcaaat actggtcctt cttttttaga
    34381 atctatagtt actataatac cagttcctgc aactccttta ttaatatttt caaaccccat
    34441 tttttcaagc tcttttaaaa caaactctga tgtcttatat tcttcaaatc ctttttctgg
    34501 tatagtgtgt agataatcat acactttatg tacatactca atataatctt tgttagttcc
    34561 cataatctag ctcctcctta aaatttaaac tttccattta tattaaattt ttattataat
    34621 tacctatact acgaaataat tcattataag cattgcaata caaccattta aagttgctat
    34681 aacaaacata tgtggataat atttagtttt taactcagct gttcccaaaa gtctccccat
    34741 aaattgtatc tgcgctcctg aaagcaataa cattggcaat agaattgcaa catgtgcttg
    34801 acttatttga cctgctgtaa ataaacctgc gattactcca gatgctcctc ccattgatat
    34861 aaatattgtc attatagctg ttgctgcaac acctggaagt ccaaagatac tcattacagg
    34921 tgtaaatatt gtccctatta catccaaaag acctgttaaa ttgaacacag aaattatagc
    34981 aaaaccgaag atgacatttg gagccattga atttgtactt atttggaatc catttctagc
    35041 tcctccaata aacgcatcta tcacatttcc ttttttaact tttacttctt tttttgctac
    35101 ctcactcatg ttaagcttcc cccttgtcaa atttatttaa atataatcta agtaagttcg
    35161 ccccaacaaa cttcataatt aatattaaag caagtggtat aaatattggt acatctccta
    35221 taaatggaaa tagtgctgct ccagaagcaa agaaattcgt aattgcagac gcacctgaaa
    35281 attgaaatgc acaaaataca gctttttgtt tttcatctat atacccatca tctttaagtt
    35341 ctttagtcat agatgcagct gcatctgtag actgtaaact agctatcaaa gttagtcctg
    35401 caaatccagg taaccctaat aatggcttta atagtggact aagaagcttt tgagctgcct
    35461 tcattccatc taagtggtct ataactctaa ctactccaag tgcaaacata actgatggta
    35521 ttaatgtcaa tgcatatagc catccatctc gagggccagt acctccatca cctctaaagt
    35581 ttgaagctaa tgtacctaca ccatcattaa ttgttcccaa tgttccaaaa gaacctaata
    35641 cattgttaaa atccaatact tttaatggtc ctggtgcatc tttaaatatt cctgaaaaaa
    35701 ataatgctcc caatattaat gctacatacc cataccattt tacctcaact ttttctgttg
    35761 ttgattctaa tttactcata taaattctcc ctcctgttta aatgaatttt attagtatac
    35821 ttgtttaata agcaaaactt atgccaatac cttaatgata aagcctttgt atttattttt
    35881 ttattgtaaa tttattccag cgattaaata gcttataatt acttactttt caagtttaaa
    35941 a
//
LOCUS       DAELXX010000028        35919 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.028, whole genome shotgun sequence.
ACCESSION   DAELXX010000028 DAELXX010000000
VERSION     DAELXX010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35919
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.028"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            795..1817
                     /locus_tag="KPH29_001351"
     CDS             795..1817
                     /locus_tag="KPH29_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG1466973.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSIDQNVDSTNNNQQNNANTNQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFV
                     TAKRIL"
     gene            2113..3033
                     /gene="pyrB"
                     /locus_tag="KPH29_001352"
     CDS             2113..3033
                     /gene="pyrB"
                     /locus_tag="KPH29_001352"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBG1466974.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGINED"
     gene            3072..3764
                     /locus_tag="KPH29_001353"
     CDS             3072..3764
                     /locus_tag="KPH29_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBG1466975.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDNIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNGGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            3757..4659
                     /locus_tag="KPH29_001354"
     CDS             3757..4659
                     /locus_tag="KPH29_001354"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBG1466976.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETPSGMMNSVGLENPGVQGFIDYELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            4744..5328
                     /locus_tag="KPH29_001355"
     CDS             4744..5328
                     /locus_tag="KPH29_001355"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBG1466977.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            5543..6469
                     /locus_tag="KPH29_001356"
     CDS             5543..6469
                     /locus_tag="KPH29_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HBG1466978.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFNYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIGKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYILSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKDISLTEDDVENSLHRILEHSKEGRTSMLQDVEAHRPTEVDMF
                     SKNICKLGKKYSIPTPINQTLFYMIKVIESRF"
     gene            complement(6521..7411)
                     /locus_tag="KPH29_001357"
     CDS             complement(6521..7411)
                     /locus_tag="KPH29_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG1466979.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSQKYPDIYVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYNSHLLKDFSFDKLQDQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            7599..8630
                     /locus_tag="KPH29_001358"
     CDS             7599..8630
                     /locus_tag="KPH29_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBG1466980.1"
                     /translation="MLKKKSSLTLKTIREILPGLFISVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGAIVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPKEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGTVQVVS
                     AVVLIKIFI"
     gene            8924..9799
                     /locus_tag="KPH29_001359"
     CDS             8924..9799
                     /locus_tag="KPH29_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine DNA glycosylase"
                     /protein_id="HBG1466981.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     RGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDEVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            9817..11307
                     /gene="cls"
                     /locus_tag="KPH29_001360"
     CDS             9817..11307
                     /gene="cls"
                     /locus_tag="KPH29_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBG1466982.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSHCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            11500..11784
                     /locus_tag="KPH29_001361"
     CDS             11500..11784
                     /locus_tag="KPH29_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBG1466983.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            11817..13445
                     /gene="groL"
                     /locus_tag="KPH29_001362"
     CDS             11817..13445
                     /gene="groL"
                     /locus_tag="KPH29_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBG1466984.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVIVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTEAAVADLPEKEDAGMPGMGGGMPGMM"
     gene            13707..14447
                     /gene="larB"
                     /locus_tag="KPH29_001363"
     CDS             13707..14447
                     /gene="larB"
                     /locus_tag="KPH29_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HBG1466985.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEEASK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLASMINKL"
     gene            14531..15265
                     /gene="larC"
                     /locus_tag="KPH29_001364"
     CDS             14531..15265
                     /gene="larC"
                     /locus_tag="KPH29_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HBG1466986.1"
                     /translation="MSEKILYFDIISGISGDMTLASLLNLGVPKEIFLEEIHKLRMSD
                     EFNINISSKTENGIVGTKVEVITKEKHTHRNLVDIFEVIDKSTLNENVKSKAKKIFMV
                     IGEAEAKVHGTTIDKIHFHEVGAIDSIVDIVGASILVDLLCVDKVCASTVSVGSGFVK
                     CEHGIIPIPAPATIEILKGVPIKLNNVNGECTTPTGAAIIKVLCDEFVDELEFSPKQI
                     GYGIGHKKFEVPNILRTVLGEKKKKN"
     gene            15295..15714
                     /locus_tag="KPH29_001365"
     CDS             15295..15714
                     /locus_tag="KPH29_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HBG1466987.1"
                     /translation="MSSEIYSYLYEKILDEKALDIYTESIYMKKNRPATKVSILCNES
                     DLEKFIKILLLETSTFGVRYNAYNREILERKFVKLDTKYGVVTVKLGYYKGKLVKVTP
                     EYNECKTIAKNEDIPIKKVFADINFRIKEEFSENLLT"
     regulatory      15774..15875
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            16096..17631
                     /gene="guaA"
                     /locus_tag="KPH29_001366"
     CDS             16096..17631
                     /gene="guaA"
                     /locus_tag="KPH29_001366"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBG1466988.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTSITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            18170..19600
                     /locus_tag="KPH29_001367"
     CDS             18170..19600
                     /locus_tag="KPH29_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG1466989.1"
                     /translation="MILNQIFIEDGGIMRNKIIIGITFSLVLLMSGCASKQTAEINVL
                     ATTDLHGVIPYELTSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDGD
                     NNTESYIETPISKDMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTY
                     KKNGDSYTKPYIIKNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQE
                     GYNGELYMNDLVEDAKKWVGIMEKEKPDIIVAVAHTGEKPKKPRNPGNRIQDLAQQVD
                     GIDAIVAGHNHVQIEQHDYKNKSGENVIVTEPGKHGEYISKINFKLEKNNDKWNIVDK
                     SSKLIKFEKNQESDKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDI
                     YKTIGYKWRNISSPKGDDMVQIVVMNNNKPVSYIYGEAEVMPINIKCDESEYKNNVVT
                     IYPNKNDKFKIEKGKQEYSINLKHIQ"
     gene            complement(19748..20623)
                     /locus_tag="KPH29_001368"
     CDS             complement(19748..20623)
                     /locus_tag="KPH29_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG1466990.1"
                     /translation="MINIRYLEILEAIEQTGTFTGAAKKLHITQSAVSHAIAELEQQA
                     ETSLFDRLPRGVCLTRCGISLLDEARSILTACRNLDKKISRLEECTPINIVSSITIAS
                     FLLPPILSRLKNSFPSLKVNVRVESANSAMDILKSGEADICFWEGIEPQGAFRTILLG
                     SYKLCVACAPTFPLSEQTLSLRQLCTFPLLLREQGSAIRDTLDSVLSLSNQKAYPVWE
                     SVNSFALIKAAEAGLGITILPENLLLDSLLHKKLRLIELDEIDMENKMLAILHKDKNI
                     TQPLEIILDNISNVL"
     gene            20743..21306
                     /locus_tag="KPH29_001369"
     CDS             20743..21306
                     /locus_tag="KPH29_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG1466991.1"
                     /translation="MNQSRKIYFWLLIINLFISTFTFGGGYVVVPMIRRYFVNQKQFF
                     TEDDLMSMAAVAQSTPGAIAINLSALAGYRVAGTLGVVISCTAAIIPPLVILGLVSAF
                     YTTFISNAIVTAVLKGMQAGVAALIVDLIIDMCSMILKQRSLFLSTMIPIAFFANFVM
                     SINVIFILSVCCFVCVMQVFYKKRENK"
     gene            21303..21869
                     /locus_tag="KPH29_001370"
     CDS             21303..21869
                     /locus_tag="KPH29_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG1466992.1"
                     /translation="MNTIWNLFFIFLKIGLFSIGGGYAIIPLIQEKVVNQTGWISEKM
                     FTDIITISQMTPGPLAVNTSTFVGLQIGGFWGAVAATLGCVLCGVVISLFLYNFFQKH
                     QNSTYIFEILNGLKSASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFV
                     ILLIVRKWKINPIVIIIISGILGIFFYL"
     gene            complement(21998..22234)
                     /locus_tag="KPH29_001371"
     CDS             complement(21998..22234)
                     /locus_tag="KPH29_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1466993.1"
                     /translation="MLECFIAEDSKIKDGVNIPTLVFKTLHDRYFKTQYFDSYYNSLK
                     CKKKLVEINNVHNSYYIDSEKFCQEAYDWFVENQ"
     gene            complement(22544..25972)
                     /gene="uvrA"
                     /locus_tag="KPH29_001372"
     CDS             complement(22544..25972)
                     /gene="uvrA"
                     /locus_tag="KPH29_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG1466994.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFENELTYEDHWLVEYYDTLTKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCRQGHAHPDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNIKIEDLNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFAKAINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLGNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKTPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLRGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNQLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPNAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKISSQNKIIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPNKNYTALTGFDAIDDFILMDQTPIGKSSRSTPATYINIF
                     DEIRSLFTETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCNEC
                     QGKRYQKEILSIQYKGKTIADILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
     gene            26291..28525
                     /locus_tag="KPH29_001373"
     CDS             26291..28525
                     /locus_tag="KPH29_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG1466995.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGI
                     IDLEGKAEFLSGKKFDLKNTASYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSNKESMDEILNIDSFNGEGTIEIINSQGKTLFIGKNNELIKDLSYKYKDSADEP
                     WAQKMLNDFKNDKSGNITITSSKGVQCLLTYHPITRGLNDWHFLLIVPEDVVLGELNK
                     LNSFTIIMTFITTCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMIAMNIKDFKLINDCFGSEKGNMTLKHLYNILYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSNFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENPEAID
                     IIEDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIHY"
     gene            28781..30811
                     /locus_tag="KPH29_001374"
     CDS             28781..30811
                     /locus_tag="KPH29_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG1466996.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKSFKISGEEDLIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENEIILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKEWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDEDMKWEDAVKISSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            30808..31260
                     /locus_tag="KPH29_001375"
     CDS             30808..31260
                     /locus_tag="KPH29_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG1466997.1"
                     /translation="MNKLVNVKCSFKIHANSKEEAIVSLVDVVSKEGYLIDKNQFLED
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDIPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            31262..32317
                     /locus_tag="KPH29_001376"
     CDS             31262..32317
                     /locus_tag="KPH29_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBG1466998.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLIGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     IGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDESIELIFEEL"
     gene            32384..32692
                     /locus_tag="KPH29_001377"
     CDS             32384..32692
                     /locus_tag="KPH29_001377"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBG1466999.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            32796..34061
                     /locus_tag="KPH29_001378"
     CDS             32796..34061
                     /locus_tag="KPH29_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HBG1467000.1"
                     /translation="MKKLPIKTVVQRLLDLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKIADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLSVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVDKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKNEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            34149..34865
                     /locus_tag="KPH29_001379"
     CDS             34149..34865
                     /locus_tag="KPH29_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBG1467001.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVERILNFSKERNINLGIITNGPSNHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGHEVEELFYKPDFV
                     ILEHKDLISLFIKICSYKNM"
     gene            complement(35060..35353)
                     /locus_tag="KPH29_001380"
     CDS             complement(35060..35353)
                     /locus_tag="KPH29_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HBG1467002.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(35359..35574)
                     /locus_tag="KPH29_001381"
     CDS             complement(35359..35574)
                     /locus_tag="KPH29_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467003.1"
                     /translation="MRTLSETEKISLAAVIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAIQQFIVENEILISEEV"
BASE COUNT        13684 a         3965 c         6283 g        11987 t
ORIGIN      
        1 acttataaag aacttaaata agtgaaataa attattaatt ataaggttta taattttaaa
       61 aaattagata aattcaggtg taaaatacta taaaacacaa ttcggaaaag tgtatgttga
      121 acttcattta aaatagaatt ttcaaagttg ttatagtaag tagtttaagc attcttctta
      181 tattaatctt tgagaataat gtacttaaaa aagttagtaa aaaatttaac tattgtatat
      241 taatgatagt tgctattgat atattatcta aaaaaattaa gtatgaaaag attactgcta
      301 ggacaatctt ttcatactta attttttaat atttatattc tccaaaattt tcatcttttt
      361 aacttagaat tattagaatt tgagaatcac tattaggaaa taggttttga tttatgcagg
      421 gttaaaaatt tcacagaata tctaacttta tactaaatcc atcaaaaaat atacgccata
      481 taaaagcgca taaaaaaaca cataaaacaa tatttatgcg cactttaacc tcgaatggta
      541 caaaattctt tacttttaca gacccatatt atataattaa gtaaataatg tatatttagg
      601 tgacatattg gataagctaa tttatttatt ttattaatat aatgatacta taaaagttat
      661 ataaagtggt ttacaaaaaa gttacagata gagcttttat taatgtattg tgtattagta
      721 ttttatcctt aggtacactt cacctacaaa atagaatcat aagataatta tgggaaaatt
      781 ttaggaggtt tatcgtgaag aaaaagatat taataccagt atttgcatca gttatggcat
      841 tatcagtaag ttcaattgta aatgcagatg aagtaaatga ttcatctcaa aataaggatg
      901 ataaaacaaa tactgaatta aatttaggtg aatataaaga agtaaaatat aaagttgcaa
      961 aaataaaaga tggagtagct ataaagataa gagaagaagg tcaagttcaa aacatagctt
     1021 attctggaga tgaatttacg gttttaggaa ctcaaggaga atgggttaag gttaaagttg
     1081 aagatggcga aggttggtta gctactagat atgtagatat atctgaaggt gtaggatata
     1141 ctaacgctga taaagtaaac ttaagaaaag ataagagtga aagttcagaa gtaattgaag
     1201 agctggaaaa aggaagttct ttattggttt tggaggataa tggagattgg ttaaaagtta
     1261 aagatgggga aacagaagga tatgtaaaat catcttatat atctgataaa gctcctgtaa
     1321 tagaagagcc acaagtaaac ccaagtattg accaaaatgt agattctaca aataataatc
     1381 aacaaaacaa tgcaaatact aatcaacaaa ataataattc aaatgtgcca acagctaact
     1441 ctaatgcagt tcaagctgta cttaacttgg catattctaa gcaaggttgt ccatatgttt
     1501 ggggagcaga aggaccaaac acttttgact gttctggatt tacacaatat gtgtatagaa
     1561 atgcagtagg aaagaatata ccaagaacat ctaaagcaca atctaaatat ggacaaactg
     1621 taagtaaagc aaatttacaa ccaggagact tagtattctt cacaactaat ggttctggtt
     1681 ctgttagtca tgttggaata tatgtaggtg ggggtaatat gatacattca ccatcaacag
     1741 gaaaaacagt tagtgtaact agtataaatt catcatacta tacagcaaga tttgttacag
     1801 caaaaagaat attataatcg gtttgtaata tgacaaattg ataaatacat aaatatatat
     1861 aaacgctcta tattatattt aatttaatca tggaaacaaa aaatgattat attttaaatg
     1921 tactatagag cgtttttgta tcactttaaa tctaatcaag attcaagtta atatgactat
     1981 taaataggag ccttttcata tgatgattat ttgcgcaatc ccttgacagg atagggtaaa
     2041 ggtggtaagc tactaacagt acaaaaatac ttttaatttt agttcagaga ggctaaaaaa
     2101 ggaggacgaa agatgttaaa atcaagaaat ttaatccaac cagaagactt ttcaatagaa
     2161 gaaatagatg aaatattaga attagctcaa aaaataattg acaatccgtc aaaatattct
     2221 cgtatatgtg aaggaaaact attagctaca ttattttatg aaccatcaac aagaacaaga
     2281 ttaagctttg aatctgctat gaacagatta ggaggacgtg tagtaggctt ttcagagcca
     2341 aattcatcct ctgcatcaaa aggtgaaact ctaggagata caatgaggat agtatctggt
     2401 tatgttgata taatagctat gagacatcct caatcaggtg cagcttcaga agcagctaga
     2461 tatacggaag tgccatttat aaatgcagga gacggcaaaa accagcatcc aactcaaaca
     2521 cttacagact tacttactat aaaatcatta aaaggaactt tagaaagcca tacaatagga
     2581 ttatgtggtg atttaaaata tggaagaact gtgcattctt tggttaaggc tatggcaaga
     2641 tataaaaata caaagtttgt atttatagcg cctgaagagt taaaaatgcc agattatata
     2701 aaagaagcta taaagggaca tgcatattat gaaactaata atttggatga tgttatagga
     2761 agcttggatg ttttatatat gactagagtt caacaagaaa ggtttgaaga taaatcagag
     2821 tatgaaagac ttaaaaacta ttatatttta aataaagcta aattagaaaa ggcatctaaa
     2881 gatatgttag taatgcatcc attaccaaga gtaaatgaaa tagacataga tgtagatagt
     2941 gatgatagag ctgtatattt taaacaagct aaatatggaa tgtatgttag aatggcttta
     3001 ataataaaac ttttaggtat aaatgaagac taatagttat catataatag taaaacaaag
     3061 gagattttgg gatgtataaa attctggaaa atatatatat aggcgaagat atgtatagaa
     3121 tgaaagtaaa aggtaatttt gaaggtaaaa tgggacaatt ctatatgtta agagcatggg
     3181 atacttatcc agtactttca agacctataa gtatacatga tatagatgaa gaagggataa
     3241 cttttcttta taaagtagta ggagaaggaa ctcaaatttt gagtaaccta aaggtaaatg
     3301 ataatataaa attagaaggc ccttatggta atggatatgc taaggttgat ggaaaggtag
     3361 ctctagtagg aggaggaatc ggtgtagctc cactatattt ggttgctaaa aatataaaaa
     3421 attgtgatgc atatcttggg tttagagaag atgttatatt agaagatgaa tataagcaag
     3481 tatgtaataa agtctataca acagtaggaa atacttttgt aacagatata attgatgttg
     3541 agaaatatga ttatatatta acatgtggtc caactccaat gatggaaaaa ttagttaaga
     3601 tggtagaagg tactaaaacg agaattatgg tatctcttga aaaccatatg gcttgtggag
     3661 taggtgcctg tttagtatgt acgtgtaaaa ctaatggagg aaacaaaaag acttgtaaag
     3721 atggtccagt gttctgggga gaggacgtga tttttaatgg gtaatttaag tgtgaaattt
     3781 ggaagtgtag aatttaaaaa tccagtaata atggcgtctg gaacttttgg atttggaaaa
     3841 gaatataatg agatttatga tatacaaaaa cttggtggta taagtagcaa gggtcttact
     3901 ttaaataaaa aacctggtaa caatggtatg agagttcatg agacaccatc cggtatgatg
     3961 aatagtgttg ggcttgaaaa ccctggagtt caaggattta tagactatga attacctttt
     4021 tttagtaaac tagacttagt cagaatagct aatgttggag gtgggacatt agaagactac
     4081 ttattagggg ttcaaatgtt aaatgacaag cctatagaca ttattgaatt aaatatatct
     4141 tgcccaaatg tcaaagctgg tggaatggct tttggtataa aaaatgaagt ggcaagagaa
     4201 gttgttcgtg aggtaagaaa cataacgaaa ctgcctttag taataaaatt atcacccaat
     4261 gcagaagata ttgtaggaat ggcaaaagtt tgtgaagaag aaggtgctga tggagtttca
     4321 ttagttaata cttttaaagc aatggcaatt gatataaaaa atagaagacc agtatttgaa
     4381 aatgtatatg ctggattatc tggaccagct ataaaaccta tagctttgag aatggtacat
     4441 gaagtgtgta aaaatgtaaa tatacctgta atgggaatgg gcggaataac taaagccaca
     4501 gatgctatag agtttataat ggcaggagct acttgtatcc aagttggaac tgcaaatttt
     4561 atgaatccta gaataggtat tgaaataata gatgggatta atgaatttat ggatagagaa
     4621 ggaataaaaa gtttagacga aataagagga attatataag gtagaataaa tagtatttta
     4681 aagataaaaa atatagatta aataaacaat aattttataa cattaacatg gaggaaaaat
     4741 aatatgagta aagtagatgt agtggatata ttaaagaaaa gtgatgcatt gttggaggga
     4801 cattttttat tatcttcagg aaaacacagt aatagatacg tacaatgtgc aaaagtatta
     4861 agatttccag aatatgctgc tgaggtatta agtacagttg ttgaacaaat aaaagactta
     4921 gatatagact tagtagtagg accagctatg ggtggagtaa tagtttctta tgagttagga
     4981 agacaattag gaaaagaagc tgtatttact gagagaaaag acaatacaat ggagttaaga
     5041 agaggatttg aagttaaaaa aggagcaaag ataataattg ctgaagacgt tgtaactact
     5101 ggtaaatcaa ctatggagac aaaaagagta ttagaagcct taggtggaga agttgtaggt
     5161 gttgcatgta tagcagatag aactaatcat gatataggta tgcctatata tagtgctata
     5221 aaacttgata ttcaagttta tgaatctgat gagtgtcctt tatgtaagga aggaaaatta
     5281 ccagttgtta agcctggaag tagagagttc aaggaattag ggatgtaata aaaacttaat
     5341 tatttatagt gttacttaaa aaatgtaaat tttttagagt aaaaatttgt aatataacat
     5401 ataacttaaa aataaaaagc agttagcctt ataaatgaag gttagctgtt ttttttgtta
     5461 aaaatatatt tacaaaataa gagatagtag cagtacaata taagtataag gtaaaagtat
     5521 tatatattag ggaggggtaa gtatgaaaaa tcaattaaat gaaaatcata aaaaggttgt
     5581 attttttgga gtaggtgctg taggagctac ttttgcagaa caatttttta actctaaata
     5641 tgattttaaa attctttgtg ataatgaaag aaagaaaaga tatttagaag aaggatttat
     5701 aataaatgga aaaaggtatg attttaatta tgtaactaaa gatgagtata aacaagaggc
     5761 tgattttata attataggtc taaaatataa taatttaaaa gaaaatataa aagaattaga
     5821 tggattagtt ggaaaaaata cagttataat gtctctgcta aatggagttg atagcgaaga
     5881 gataatagga gaaagatttg gaattggaaa aatggtatat tcatatgtta ccaatataga
     5941 tgcaaagaaa atcaataata atattataca cactactaat gggataattg tatttggtaa
     6001 caaagataat agtgaagata gaaaaactaa tataataact gaagtctttg atgatgtaaa
     6061 tatagaatat attttatcaa aagatattca acgagatatg tggtggaagt acatggttaa
     6121 tattggtgta aatcaaactt cagctatact tggtgcacct tatggagttt ttcagagttc
     6181 tgagcattta agagaattag caaaatctgc aatgagggaa gttgttgcta tagcacaagc
     6241 aaaagacata tctcttacag aagatgatgt tgaaaattca ttacatagaa tactagaaca
     6301 ttcaaaagaa ggaagaacat caatgcttca agacgtggaa gctcatagac ctacagaagt
     6361 agatatgttt tctaagaata tctgcaaact tggaaaaaaa tatagcatac ctactcctat
     6421 aaatcagact ttattttata tgataaaagt aattgaaagt agattttaaa gggtatttgt
     6481 aagaaatatt gataattatt gattttaaat tttattaatg ttatatacta tttaagttac
     6541 taaatatttt taactcttca atgaaaatag ttgctgcttt tgacaaagtt atatcttttg
     6601 gaaatatata tgagaaatgt ctgttgtagc tgcttccaag ctctatgaca gacagctcat
     6661 tgtttaaaac aggtaagctt gtcactagat tagaaactat tgtaattcct aaattatttc
     6721 taacagattc tttgacagca taattacttc caaaaaccat catacttttt ggaataattt
     6781 ctttaacact taaaaacata tctaaatagt ctctggttcc agaaccatcc tccctgacta
     6841 tccatttttg gtcttgaagt ttatcaaaac taaaatcttt taataaatga ctattgtatg
     6901 gaagtgctaa aaccatctta tcttcaaaaa aatattcttg aataaatgaa ggggaagagc
     6961 aagtgccttc aataagacca atgtctaaaa tataatcctt aacatgagaa gatacgattg
     7021 atgtattttt tataaaaact tctacatata tgtcaggata cttttgagaa aataatgcta
     7081 aaaaatttgg tagaatatat tctccaatag ttaaacttgc accaatcttt aagtgaccag
     7141 ttatagcatc tgaaacttgg cttacatcat gataagtaat atttaaaaga tttaatattt
     7201 ctttagctct tttgtacaat atttgtccac tttctgttat aaaaatggtt ttttgtttaa
     7261 tagacctgtt tataagcaca actttaaaat aattctctag attttttatg tgtttactta
     7321 cacttggttg tgatagattt aagtattccc cagctttagt aaagtttttg tattcaacaa
     7381 cagctacaaa tgtttttaat tcttcaaaca attttttgtc tccttcacct aatgaaattt
     7441 tacatttttt atatactttc tacagataat tatactatat ataataacca ttaaaaacat
     7501 taatcattac gatattatat atttatttta cttatggtta ttacaattat atactattgc
     7561 tatgaagaaa actatattaa ttataaggta ggtatattat gttaaagaaa aaaagcagtt
     7621 tgacattaaa aactataagg gaaatattac caggactgtt tatatcagtg ttagtaggtt
     7681 atattagtat atttatttct attttaattc ctaaggtagg ggcagcatca atttctatat
     7741 ttttaggtat gtttgtagga aacctatttt taaatcaaaa ggtatttcaa aaaggttata
     7801 aattttcaga gacagatttg ttatcatatt caatagtact attaggggca acactcagtg
     7861 tatctacctt aatagactta aaagtttcag gaattttatt tataatattg caaatgacaa
     7921 taacaataat agctgcactt tatataggta aaaaactagg atttgaagaa aactttagat
     7981 ttctaatggc aagtggaaat gcagtatgtg gttcttctgc cataggagca atagttccag
     8041 tggttaatgc gacagataaa gaaaagggaa ttgcagtaac tattgtaaat gttactggta
     8101 tattccttat gtttttactt ccaataatat ctcaattttt atatagccat gaacttgtaa
     8161 aaacttcagc aatgataggt ggaactttgc aatcaattgg tcaggttgta gcaagtggag
     8221 caattgttgg agaaaatgta aaagatttgg ctactatttt taagatagta agagtaatat
     8281 ttctagttgt tgttgtatta gtatttggac atatgaaaaa tcagtctaat agggaaatcc
     8341 ttgaagaaga aaaggatgag ataaaaaaga aaaaagtaac tataccatgg tatgtaattg
     8401 gattctttat aacttgtgca cttttttcaa tgaacattat accaaaagaa gtatctgtgt
     8461 tgtgcaaaga aataagtaac aaactagaaa taatagcttt agcagcaatt ggattaaaag
     8521 ttaatgtaaa agatttagtg aaacaaggga aagaagtttc tttatatggt ttatttgtag
     8581 gtacagtaca agttgtatca gctgtagttt taataaaaat atttatatag cacaaaaata
     8641 gtatttgtaa tattaatttg attaattaaa ataatatgat actaataatt tataatataa
     8701 ataaaaaatg aatttcgata agtttaagtg ggtataaact tatagaaatt cattttttat
     8761 ttaaggtaaa aatttaaagt aaaatattat tttacataat aataacctta taaattttga
     8821 aattacttat ctttgtgtta aaataagtaa aatacacata ttaaagttaa gtttttaggc
     8881 aaataaacaa aatgaaaata taaatattaa ggagatttgg tatatgaatg tttatgaaaa
     8941 aggtaatgga gttatactag aaggagtaac agattttgac ccagtacata tatttgagtg
     9001 tggtcaatgt tttagatggc acaaacaaga agatggctca tacactggag ttgcaagggg
     9061 gagaatatta aatgtcaaaa aagagaatga taaaatatat ttaaataata cgaatttaaa
     9121 ggagtttaac agcatttggt ataattactt tgatttagga actgattata cagaaataaa
     9181 gaataagcta aaaaatatgg atgaatattt gaataaggct actgaatttg gatggggaat
     9241 aagaatatta agacaagatg gatgggaaat gcttatatca tttataattt cctctaataa
     9301 tagaattcct atgatacaaa gagcgataga aaatttatca agaaagtttg gtaagtatat
     9361 aggtgaatat gaaggtaatg aatactatgc attcccaaca ccagaagaac taaataaggc
     9421 ctcacaagag gaaataagag catgtcaaac aggttttaga gataagtaca taaaaagtac
     9481 tacacaggca gttatagaga ataatgatga ggtatctgaa tatacaaatt taagtacaga
     9541 ggattgtaga aaagagcttt taaaatttaa tggagtagga ccaaaagtat gtgattgtat
     9601 tgcattattt ggtatgcaaa aatatgattc ttttccagtt gatgtttggg ttaagagagt
     9661 tatgcaagaa ttttatatag atgaagacat gagtttacct aaaatgagaa cttatggaat
     9721 cgataaattt aaagaaatgt ctggatttgc tcaacagtac ttattttact atgcaagaga
     9781 actcggtata ggaaaataaa ttaaggggga gaaattatgg gagtaatagg aacaatattt
     9841 ctgttctatt taattatatc atatctagct ggagccataa tttctgtgat tatattgctt
     9901 gaaaatagag accctgcaaa aacaatgtct tggctattga tgtttattat atttccaggt
     9961 gtaggtttaa tgatatatgc tatttcaggt agaaatatta gaaaaagaaa attatttaag
    10021 actcaaaaac tagctaataa tataaaagaa aagaaactat ttgatacatt agaaaagata
    10081 acagaaatag ttgaattaga aaaagaatct ataaaacaaa ataagctttt aagagatgaa
    10141 gaggatggta gttatagaaa aagggtaata aatatgttgt taaagactgg tatgtttccc
    10201 tttactaaaa ataataaagt ggatgtattt gtagatggaa atgaaaagtt taaaagactt
    10261 atagaagata taagagaagc taaagaccat attcatttag agtactttat catcaaagat
    10321 agtgaaatag gtagggtgtt aaaagaagaa cttataaaaa aggctaaaga aggcattaag
    10381 ataagaattt tatatgatga tgtaggatgc tggagatttt ggtttaatag aaaatttttc
    10441 cgagaaatga gggaagtagg tatagaaatt gcagcatttt tacctacaaa atttcctata
    10501 ataggaggga aattaaacta tagaaatcac agaaaaattg ttgttattga tggaataatt
    10561 ggatatacag gtggaataaa tataggagat gaatatttag gcaaaaatga taagtttgga
    10621 tattggagag atactcatat taggattaag ggtatttctg tatacatgct tcaaatgaca
    10681 tttctgattg attggtatta tacgacaaaa gaagtattag tgactaaaaa ttatttccca
    10741 agtgtaggga atgttggtga aagtatgata caagttgttg cgagtggtcc agacagtgac
    10801 tgggaagata ttcattatgc ttatttttct gcaatatgtc aggctagaaa aaatgtttat
    10861 atagaaactc catattttat tccagatgaa agtctactaa aagctataaa gagtgctgct
    10921 cttagtggag ttgatgtgag aataatattt cctaaaattg cagaccacaa gatagtaaat
    10981 atagcatctt attcatattt tgaagaaata ttaagagcag gtggtaaagt ttatttatat
    11041 aataaaggat ttatacattc taaagtcgta ataatagatg ataaaattgc ttctgcaggt
    11101 acagctaata tggatttaag aagttttatg cttaactttg aggtaaatgc atttatctat
    11161 gatgaagaag ttattagggt aatgacagat gatttctttg aggatttaag tcactgtgaa
    11221 gaacttaatt tggaggtatt taagaataga aatatcatac aaaaaatcaa agaatctgta
    11281 gcaagattat tttcaccaat attataaaaa attattaaaa aaggtattga aaattatgtg
    11341 tgattggtat attataataa ttgttagcac tctattagat agagtgataa taaataaaaa
    11401 gtatatatat aaatctacat atataagtag tattaattta aaaataacta aaaacatagg
    11461 cgaactagtc tatacttaat attttaggag gcgtgaataa tgaaaataag accattagct
    11521 gacagagtag taattaaaaa agtagaagca gaagaaaaaa ctgcaagtgg aatagtttta
    11581 ccaggagcag ctaaagagca acctcaaata gctgaagttg tagaagttgg accaggtgga
    11641 atagttgaag ggaaagaaat aaaaatggaa ttgacagtgg gagataaggt tatattccaa
    11701 aaatattctg gaacagaagt taagatagaa ggacaagaat acacaatact aagacagagt
    11761 gatgtattag ctgttattga ataaatatag aataaattta ttaggaggga tttaaaatgg
    11821 ctaaagaaat taaattttca gaggaaacaa gaagagcttt agaagctggt gtaaataaat
    11881 tagcagatac agtaaaagta acattaggac ctaaaggaag aaatgttata ttagataaaa
    11941 aatttggttc tccacttata actaatgatg gagtaactat agcaaaagag atagaactag
    12001 aagatagatt tgaaaatatg ggtgctcaat tagttaaaga agtagcaact aaaactaatg
    12061 atgtagctgg agatggtact acaactgcta cagttttagc acaagctata ataagagaag
    12121 gtttaaagaa tgtaacagca ggggctaacc caatactttt aagaaaagga atacaaaaag
    12181 cagtaatagt agcggtagaa gaattaaaaa atcaatcaag aatagtagaa acacaagagg
    12241 ctatatctca agttgcttct atatctgctg gtgatgaaga agttggaaaa ttaatagcag
    12301 aagctatgga aatagtaggt aaagatggag ttataactgt tgaagagtct caaactatga
    12361 atactgaatt agatgctgtt gaaggtatgc agtttgatag aggatttgtt tctgcatata
    12421 tggttacaga tgtagataaa atggaagcag ttttaaatga cccatatata ttaattactg
    12481 ataagaaaat atctaacata caagaattat taccagttct tgaacaaata gttcaacaag
    12541 gtaaaaagtt attgataata gctgaagatg tagaaggtga agcattatct acattagtag
    12601 taaataaatt aagaggaaca tttgatgtag tagcagttaa agctccaggc tttggagata
    12661 gaagaaaaga aatgcttcaa gatatagcaa tacttacagg tgctcaagta atatcagaag
    12721 aattaggtta tgatttaaaa gaagctgatt tatctatgct aggtagagct tcatctgtta
    12781 aggtaactaa agaaagtact acaatagtag atggttctgg agataaaaaa gctatagaag
    12841 atagagtaac tcaaattaaa catcaagtag agcaaactac ttcagatttt gatagagaga
    12901 aattaatgga aagattagct aaacttgctg gaggagtagc tgttgtaaaa gttggagctg
    12961 ctacagaagt agagttaaaa gaaagaaaat taagaataga agatgctctt aatgcaacta
    13021 gagcagctgt agaagaagga atagtagctg gtggaggaac tgcttttgtt agtgttatac
    13081 cagcaatagg aacactaatt gagagtttag aaggagaagt taaactaggt gctcaaatag
    13141 ttaaaaaagc gttagaagag ccattaagac aaatagctat aaatgctggt cttgaaggtg
    13201 ctgtaattgt gcaaaatgtt gttaattctg aagcagaaac tggatttgac gcattgaatg
    13261 aaaaatatgt aaatatgata gaagctggta tagttgaccc aactaaagtt agtagaagtg
    13321 ctttacaaaa tgctgcatca atagcaagta ctttcttaac tactgaagct gctgttgctg
    13381 accttcctga aaaagaagat gcaggaatgc caggaatggg tggcggaatg cctggtatga
    13441 tgtaatttaa aaaatttaaa atataaattt taatataaag aactatttcg acttagattt
    13501 taaactctaa gttgaaatag ttcttttgta tatatctaat ttttgtatat ctataaattc
    13561 aatacacata tttagataat cagtatctag tttgtaataa tattttagag tagtattgtt
    13621 attttttagg ttatgatttt agatatatat taattaaggt ataattgttg atagagaata
    13681 aatcaaattt aaaaggatgc gataatatgg acttaagaaa attacttgaa gaagttaaaa
    13741 atgaaaatat tgatatagat tttgcgttag aaaagttaaa agatttacct tatgaagacc
    13801 ttggatatgc aaatatagac catcatagag aattaagaaa tgggtatccg gaagttattt
    13861 attgtgaagg taagagtgat gagcatattt tagggattat tgataaaatg aatcaaaaaa
    13921 gttctaacat attggggaca aggtgcagaa aagaaacttt tctgaaaatt aaagaaatct
    13981 ataatcatgc tgagtacgaa gaggcgtcta aaattcttaa gattcaaaat catgatatag
    14041 agaatatagg aaaaggtaaa attgtgattg ctacaggggg aacttctgat ataccagtag
    14101 cagacgaagc ttatcataca gctaagtttt taggtaatga tgttgataga atatatgatg
    14161 ttggagtggc aggtatacat aggcttttaa ataaaagaca taaaatagat agtgctagag
    14221 taatagtagc cgttgcaggt atggaaggtg ctcttgcaag tgtaattggt ggactggttg
    14281 atgttccagt aatagctgtt ccaacttctg ttggatatgg agcaaatttt ggaggattag
    14341 cagcattact agctatgtta aatagctgtg catctggaat atctgttgta aatattgata
    14401 atggatttgg agcaggatat ttagcatcaa tgataaataa actgtaaata cattgatata
    14461 aattaaacaa aactatataa taaaatttaa tttctattat atttttacta ttaaaattga
    14521 ggagacatat atgagtgaaa aaatacttta ttttgatata ataagcggta tatcagggga
    14581 tatgaccctt gcaagtttat taaatttagg tgtgcctaag gaaatttttt tggaggaaat
    14641 tcataaacta agaatgagcg atgaatttaa tataaacatc agctcgaaaa ctgaaaatgg
    14701 aatagtaggt acaaaagtag aagtaattac aaaagaaaaa catacacata gaaatttagt
    14761 ggatatattt gaggttatag ataaaagcac tctaaacgaa aatgtaaaat ctaaagcaaa
    14821 aaaaatattt atggtaattg gagaagcaga agcaaaagtt catggaacta caatagacaa
    14881 gatacatttt catgaggtag gggctataga ttcaattgta gatatagtag gagcttctat
    14941 attagttgac ttactttgtg tagataaagt ttgtgctagt acagtgtctg ttggttcagg
    15001 atttgtaaaa tgtgaacatg gaattatacc tatacctgca cctgcaacta tagaaatttt
    15061 aaaaggtgtg ccaattaaat taaataatgt aaatggagaa tgtacaactc caacaggagc
    15121 tgcaatcata aaagtcttgt gtgatgagtt tgtagatgag cttgaatttt ctccaaagca
    15181 aattggatat ggtataggtc ataagaagtt tgaagtacca aatatactca gaactgtatt
    15241 aggtgaaaaa aaaaagaaga attaatttat gaaattagtg caaacattga tgatatgtct
    15301 tcagaaatct attcttattt gtatgaaaaa atattagatg agaaagcatt ggatatttat
    15361 acagagagta tttatatgaa gaagaataga ccagcaacaa aagtatcaat tttatgtaat
    15421 gaatcagatt tagaaaagtt tattaaaata ttacttttag aaactagtac ttttggagtg
    15481 agatataatg catataatag agaaatttta gagagaaaat ttgtaaaatt agatacaaaa
    15541 tatggagtgg ttacagtaaa actagggtat tataaaggaa aactagtcaa ggttactcca
    15601 gaatataatg aatgtaaaac tatagcaaaa aatgaagata ttcctataaa aaaagtgttt
    15661 gctgatataa attttagaat aaaagaagaa tttagtgaaa acttattgac ataagtaata
    15721 aattttgata aattaatatt cagccttatt aaataaaagt aaacagaaaa aaatatataa
    15781 aatataaact cactcgtata tgctcagaaa tatggtctga gagtctctac caagataccg
    15841 taaatatctt gactatgagt gaaattatta taccaaaaga cctagttaag taaatgtgta
    15901 ggctgagtta tactccctat gtgttggcta taagataaag gtattttgga agcctgtata
    15961 gtaatttcac ttttgctaga aattatggaa gttactatac aggttttttg tgtaaaaaga
    16021 tttaatataa tttagactgt attgtgttaa aataaatata taaaaactat attatacaag
    16081 gagagatgtg aatttatgaa acatgaatta gtacttgtaa tagattttgg aggacaatat
    16141 aatcagttga tagctagaag agttagagaa aacaacgtat attgtgaaat acttccatgt
    16201 actgcaagca tagaaagaat aaaagaaaaa aatcctaaag ggataatatt tacaggcggt
    16261 ccaaatagtg cgtatttaga agattctcca acaatatcaa aagaaatatt tgaactagga
    16321 gtaccaatac ttggaatatg ctatggtatt caaataatgt ctcatgtatt gggtggagtt
    16381 gtaagaaaag gtaataaaca agaaaaggaa tatggaaaaa catctataac ttatggaaaa
    16441 tcatcattat ttgaaggaat aactacaaat agtgtatgga tgagtcatac agatttaata
    16501 gaaaaagttc cagaaggatt tactatagtt gcaaatacta atgattgtcc agtagcagct
    16561 atggaaaatg tagaacgaaa cctatatgga gttcaattcc atccagaggt agaacactgt
    16621 ttggaagggg acaagatact tacaaacttc ttatataata tttgtaaagt aaaaggtgac
    16681 tggactacag attcattcat agaagataaa ataaaagaat taaaagaaaa aataggcgat
    16741 aaaaaagctt tatgtgcttt aagtggagga gttgattctt ctgttgctgc agttcttatt
    16801 cataaagcaa ttggggacaa cttaacttgt atatttgttg accatggttt acttagaaaa
    16861 aatgaaggaa atgatgtaga aagaatattt agagagaaat ttgatataaa tttaataaga
    16921 gttaatgcag aagatagatt cttatctaaa ttaaaaggag tttccgaacc agaagctaag
    16981 agaaaaataa taggtgaaga atttataaga gtatttgaag aagaatcaaa taaattagga
    17041 aaaatggatt tcttagtaca aggaacaatc tatccagatg ttatagaaag tggacatgga
    17101 aatgctgcta ctataaagtc tcatcataat gttggtggaa tcccagaaga tgtagacttc
    17161 caagaaattg tagaaccatt aagagagtta tttaaggatg aagttagaaa aataggatta
    17221 gagcttggta tagaagaagg attaatattt agacatcctt tcccaggacc aggtctagga
    17281 ataagagtta taggtgatgt aactaaagaa aaatgtgata tattaagaga agctgatgct
    17341 gtttatatgg acgagcttag aaaagcaggt ttatacagag aaatatggca agcttttgca
    17401 acattaccag atgttaagac tgttggagtc atgggtgatg aaagaacata tgcttattta
    17461 gttggtttaa gagcagttac ttcttctgat ggaatgactt ctgactggta taagatgcct
    17521 tatgatgtat tagaaagaat ttctaataga ataataaatg aagttgatgg agtaaataga
    17581 gtagtttatg atataacttc taaacctcct ggaactattg aatgggaata aaatatagaa
    17641 tgccagaaat ccacatgaat acaggatttg aggtctctct tacttctata ttatactggt
    17701 ttgatactat caatatttgc cagtatctta tggattaata agcaaaaaac atcctgctga
    17761 atggtaaatt cggcggggtg tttttatttt gttttgaaac tcctttgctc tgaaactcga
    17821 agtatttaac actgatgatg ttgttatctt ttagttcctt ttgtgtcatg ctctgaaaga
    17881 caaattgata caataaaaaa gtaatttctt aatcattgat ggagatatgt atttatgtta
    17941 ctcttgatgg taatttgagt aatattgttc atagcttttt atattcactt aattagaaaa
    18001 ccatttggta taatcataaa taaacttgat gatataatag aaaagattta taattattag
    18061 aagtatataa aagtatttta tgtctatgaa atgtatgtct ataaaacata tagatgactt
    18121 ttgtatatta atgtatgaat attgatatta tagaagaatt gagttgtcaa tgatactaaa
    18181 tcaaatattt atagaagatg gagggattat gagaaataaa ataattatag gaattacttt
    18241 ttcacttgta ttactaatgt ctggatgtgc ttcaaaacaa acagcagaaa taaacgtatt
    18301 agcaactact gatttacatg gagttatacc ttatgagtta acttcttatg taaaagaaga
    18361 aagaaaaaaa gataaaaatc taactttagt agatgcagga gatttttttg atagtggaga
    18421 agtgtttggt tctagcatgg atagttattt tagtgctaga tataaagatg gtgataataa
    18481 cactgaaagt tatattgaga cgcctatttc aaaagatatg aaagaggtag gttatgatgt
    18541 tgcagttctt gggaatcatg aatttatatc caacaataaa ttttatctag ataatatgat
    18601 atctgatttt gagaaacaaa aaataaatat tttatcggca aatacgtata aaaaaaatgg
    18661 agatagttat acaaaaccat acataataaa aaatattaat acacctgaag gaaacgtaaa
    18721 actaggaatt ttagggctta ctataaaaga agttggtgag aggaaacagt ggaaagatgg
    18781 aaaacttgtt gactctaagt ctttagaact aaaagaccaa gagggctaca atggtgaatt
    18841 gtatatgaat gatttagtag aagatgctaa aaagtgggtt ggtataatgg aaaaagagaa
    18901 gcctgacatt atagttgctg tggcacatac aggtgagaaa cctaagaaac ctagaaaccc
    18961 aggaaataga atacaagact tagctcaaca agttgatgga attgatgcta tagttgcagg
    19021 tcataatcat gttcaaattg agcaacatga ttataaaaat aaatcaggag aaaatgttat
    19081 agttactgag ccaggaaagc atggggaata tatttctaag attaatttta aattagaaaa
    19141 aaacaatgat aaatggaata tagttgacaa atctagtaaa ttaattaaat ttgaaaaaaa
    19201 tcaagaaagt gataaatttg gagaatttat ttcttcaatt aataaagtaa gtaaagataa
    19261 gaaagaaata agattatcag aagtaaagac tcctgaatgg gataaaatat atgtttttaa
    19321 atctggaaca tctgcagatg atatatataa aacaatagga tataaatgga gaaatatatc
    19381 atctccaaag ggtgatgaca tggtgcagat tgttgtaatg aataataata agcctgttag
    19441 ctatatatat ggtgaagctg aagttatgcc aattaatata aaatgtgatg aatcagagta
    19501 taaaaataat gttgtaacta tttatcctaa taaaaatgat aaatttaaaa ttgaaaaagg
    19561 taagcaagaa tatagtatta atttaaaaca tatacaatag aaacttttat cttatttaag
    19621 tttagattgt acttaaaata aagtgtgtat tattttaaaa agtgatgata attaaaatca
    19681 caacattaag gaatagtaca cactttttat gctaaaaaat agaatgttaa aaaattaaac
    19741 ctttaaatta aagtacattg cttatattat ccaatattat ttccaaaggt tgtgtaatat
    19801 ttttatcctt atgtaatatt gctaacattt tattttccat atctatctca tctaattcaa
    19861 tcaatcgcaa ttttttatgt aagagtgaat ctaataataa attttctggc aaaatggtta
    19921 tacctaaccc tgcttctgca gctttaataa gtgcaaaaga attaacactt tcccaaacag
    19981 gataagcttt ttggtttgaa agagataata cactgtccaa agtatcgcga atagcacttc
    20041 cttgttcacg taacaataac ggaaacgtgc ataactgtcg tagagataag gtttgttcag
    20101 ataaaggaaa agttggagca catgcaacac ataacttata ggaacctaat aaaattgtac
    20161 ggaatgcccc ctgtggttct ataccttccc aaaaacatat atcagcttct ccacttttta
    20221 aaatatccat agctgaatta gcactttcga cacgtacatt cactttaaga gatggaaatg
    20281 aattttttag tcgacttaaa attggtggaa gtaaaaaaga ggcaattgta atgctagaaa
    20341 caatgtttat aggtgtacat tcttctaaac gactgatttt tttgtctaaa ttgcgacaag
    20401 cagttaagat actacgtgct tcatctaaaa gagaaatgcc acagcgagta agacataccc
    20461 ctcgtggtaa tctgtcaaat agtgatgttt cagcttgttg ttcaagttca gctatagcat
    20521 gagatacagc agattgtgtg atatgtaatt tttttgcagc acctgtaaaa gtgcctgttt
    20581 gttcaattgc ttctaaaatc tctaaatatc gtatgtttat cataataaat atcctcacta
    20641 tacatgaata aaattaatta attccattaa attctatcat tttacagatt gcaaagcaac
    20701 aggtatactg aataatatga agtgaaaaga aggaggaata ttttgaatca aagcagaaaa
    20761 atatattttt ggcttttaat tattaacttg tttattagca cattcacttt tggcggaggg
    20821 tatgtagttg tacctatgat tagacgttat tttgtaaatc aaaagcaatt ctttacagaa
    20881 gatgatttga tgagtatggc agcagttgca caatcaactc ctggagctat tgcaattaat
    20941 ttatctgctt tggcaggtta tcgagtagct ggtactttag gggttgtcat tagttgtaca
    21001 gcagctataa tacctccact cgttattctt ggattagtat cagcatttta caccactttt
    21061 atatctaatg ccattgtaac tgcagtatta aaaggaatgc aagctggtgt tgctgcacta
    21121 attgtggatt tgattattga catgtgctct atgatattaa aacaacgttc tttgttttta
    21181 tcaactatga tacctattgc tttctttgca aactttgtga tgagtataaa tgtaattttt
    21241 atattatctg tttgctgttt tgtatgtgta atgcaagttt tctataagaa gagggaaaat
    21301 aaatgaatac aatttggaac ttatttttta tatttttaaa aataggcttg tttagtattg
    21361 gtggtggtta tgcaatcata cctttaatcc aagagaaagt agtaaatcaa actggatgga
    21421 taagtgagaa aatgtttaca gatattataa ctatttcaca aatgacacca gggccactgg
    21481 ctgttaatac ttctactttt gttggacttc aaattggagg gttttgggga gcagttgcag
    21541 ccactttagg ttgtgtatta tgtggtgtag taatatctct atttttatat aatttttttc
    21601 aaaagcatca gaactctact tatatatttg aaatattaaa tggtctaaaa tcagcttcat
    21661 taggtttaat tatctcagct gcggctacaa ttataatcct tgcattattt gggacgaata
    21721 aagtgtactt caattctata tttgagatgc taaactggac tgcactaata atcttttttg
    21781 ttatactact gatagtacgt aagtggaaaa ttaatcctat tgtgattata ataataagtg
    21841 gtatactagg aatatttttt tatttataag tttttggtaa gtaaaggata tctatttttt
    21901 tatatcttaa atccaaatag ccaataaagt aaggctattt ggatttttaa taaattctga
    21961 aaagataatc agaaacccat ataacattta aattatatca ctggttttct acaaaccaat
    22021 cataagcttc ttggcaaaat ttctcactat ctatatagta actattatga acattattaa
    22081 tttcaacaag tttcttttta cattttagtg aattataata gctatcaaaa tattgggttt
    22141 taaaatatct atcatgaagt gttttaaaga caagagtagg tatgttgact ccatctttta
    22201 tcttactatc ttctgcaata aagcattcta acattgtatt aattatcttt gtactatttt
    22261 tccaaatatg aggatttttc ttttcaaata ttttataaaa ttcattatca tagacttcat
    22321 tataaccttt atatttaggt ggttttactg ttatgtgaaa attaggaaga aacttaaaga
    22381 aaaaatataa taatttttat gttctgtttt atatattatt catattttat cacagataat
    22441 atactaaagt taattttttt attgtacaac tattataata attcttctat aatataagac
    22501 attctataat tttatattta agttcatgtg tatttaattg tttttatata aaatgtccag
    22561 taatactatt tggatttatt tttatttcat caggagttcc agaagcaaca atttcccctc
    22621 catttattcc actgtctgga cctatatcga taatccaatc agaagcacgt ataacctctg
    22681 tattgtgttc aataattaaa agtgaatgct gttcatttac aagttttcta aaagtaatca
    22741 acaaattttc tatatcttta aaatgaagtc cagtagtagg ctcatccaat atgtacagca
    22801 ttcgtttttt agtttttgtc cctaattctt ttgctaattt tatcctttga gactctcctc
    22861 cgcttaatcc taatgtattt tgtccaagct ttagatatcc aagtcccact tcatctaata
    22921 aggagagttt tctatatata tcagagcaat cactaaaaaa tgtttttgct tctgctactt
    22981 ccatgtcaag tatatctgca attgtttttc ccttgtattg tatagacagt atttcttttt
    23041 gatatctctt accttgacat tcattacaag ttacccattg gtctgccata tattgtagaa
    23101 taatttttgt tttacctaat ccttgacagt ttgggcattg cccctttttg ctattaaagc
    23161 taaaatagct ttcatccaat aatttttgtt ttgcttgtgg agtttcagta aatagagaac
    23221 gaatttcatc aaaaatattg atatatgtag caggtgtaga acggctagat tttccaatag
    23281 gtgtttggtc cattaagata aaatcgtcaa tagcatcaaa tccagtaagt gcagtataat
    23341 ttttatttgg gatagatttt tgtttcaatt tttcttctag agcaggaagt aaactgtgaa
    23401 atacaagaga actcttacca gaaccactaa cacctgtaat agagcacata cagtttaagg
    23461 gaatttctac atcaatattt tttaagttat ttgcatggca tccctttaag cttaaccatt
    23521 tacttggtat aattttattt tgtgaactta tcttattgta tgtactcaaa tatttgcctg
    23581 ttatagaatt tggatttttt ataatttctt gggttgtacc ttctgcaatg atgaagcccc
    23641 cttttgtacc agcatttggt cctacatcaa taatataatc agcagacagt attgtatcct
    23701 tatcatgttc cactactata acagtattac ccttgtctct taattcttct atcattttga
    23761 tgattagatt atggtctctt ggatgtaaac caatagatgg ttcgtctaaa atataggtta
    23821 atcctactaa ttcacttcct agctgacttg ataatcgtac tctctgcagt tctcctccag
    23881 ataaagttgg agctgttcta tctgttgata tatagctaag acctaccttt aatagataac
    23941 taactcgaat ttttaattct gttaagatat catttaccaa agatagctca tttttttgaa
    24001 gttgatttgg taattcacac aaccaattcc ataactgttc tatcgtcatt tttgttagtt
    24061 cagggaagcg ataacctttg attgtagtga atctagcctc aatgcacagc aattcgccac
    24121 cacatgtatt gcaaggaatt ttgttcatat attgatgcaa ggtattattt tcagaactag
    24181 attctctaaa aagtctttga atgtggttga ttgctcctga agcaggcctt ctaatctttg
    24241 attctcgtcc ctttgattca aagctaaatt catatatctt atcgtctgta ccatataaga
    24301 ttgcatctat aaaatctctt ggcaattcat tccaaggtat atctaggtct atatttttat
    24361 cttttgctat tgcatataat tcacctacca tccaattacc atttggcttt ttccccctca
    24421 attttccaaa atagtaagta gctccatcta atattgaaat agttgggtct tttacaatta
    24481 attgaggatt aacttgatag gtaaaaccca atccattaca atcgtgacaa gctccagtat
    24541 gggagttagc attgaaggtt gaagtattta tcttagaaaa aatagtttgg caatgtggac
    24601 aataattttt tgtattattc tttttttcta atggagtttt gcaatatgga cagtaacttt
    24661 ttccgattga tacaaataac attcttaagt aatcccaaat gcttgtaaga gtaccaacag
    24721 tagaacgagg attttggctt ccttttttct gttcgatagc tatagtaggg gttaatccct
    24781 gaagtaaatc aaattccggt ttttgaagct gttctgaaag ttttgctcca ttacctagat
    24841 tttcaaaata acgtcttttt ccttcttcat aaatcgtatc gaaagctaaa gaagattttc
    24901 cactaccact tacacctgta ataacagtta tttgattttt tggaattgta acctcaacat
    24961 cctttaaatt gttttgcctt cctcctttta gatagataga gcttttatta tgacaaagtg
    25021 aatttttaat tgatttattt attgcttttg caaatttgaa tttcttttct agttcaagag
    25081 gattgcctaa ttcttttatt tccgaaatac ctaatatttt taactgagaa aagattgatt
    25141 tcaaataatc ttcaggcaat ttttctatta acaaattaag gtcttctatt tttatatttc
    25201 cgcttgataa tatctctgcc atttcatcta ataaattttt ttctgctctt gttaattgat
    25261 tgtatttttt ggaacttaat tctgtccata aatcccatgg aagcaattct aaaccaatca
    25321 tattattaaa gtcatgtaga agattaccct ttacagaatg ccaacctttc caatttttat
    25381 tatatccaaa gtcatctgga tgagcatgtc cttgtcgaca tcttatccag gcttctgaac
    25441 cagttaaaaa accatctgtt tttccgacat catgagtgtc aaaattaatt tgaaaataat
    25501 ttttttgtat gtcatctatt tgtgcatcta ttcttatcca tctctttgtt agagtatcat
    25561 agtattctac aagccagtgg tcttcataag taagctcatt ttcaaaataa ttagcaaaac
    25621 ctgctctcat tctagcctca aaacctcgat atcttaggag cgatacaaga agtaaagaat
    25681 aatctctgca cattccaaca agcctttgtt ttgggtttct tggaatagtt agtgggttag
    25741 aatttatttc aaatattctt tttaacattt gaggaattgt acgaagcaat tcctcatcag
    25801 attgttgctt attaaaggat ataccatata attttccttg gtctccatga attactaggc
    25861 tttgaaccac ttttaataac tcaattggag tctttggtaa atcttgatat ttattttcaa
    25921 attttttagg atttgtgatg atgctttgat ttttatagtt atttattttc atttatattt
    25981 cacctctcta aatggatgat ataccctgac atggtgtcat atgcaaggac aatttagatt
    26041 ttttggccaa aatttaaaaa taaataaaaa atgatacagt gataaattgt aatacaaata
    26101 tagtttcaaa ttgcatataa tataattatt taataaaacc attatctatg gaatgttaat
    26161 gaaatgttaa gatggcattc ttgaatatta agaaatagaa tactataata tagtgtgaag
    26221 gattcttagt aaaaaaattt gtaagtgtta atatacatta aaaaataggt ttataatttg
    26281 gaggaaaaaa atgaaattat caaggtataa gaaaaatagt ttagttctgt ctataatttt
    26341 atgttcactt attatttcaa tccttgtaat tactgttttt aataccgtaa aactacacaa
    26401 tatatctaat aaacaatcta aaaactattt aaacgatgta tctacacaga ttgtaatgaa
    26461 tgttgattct aaaatcaaat ttattttatc cgacttaaga ataatggcag attttattaa
    26521 gcaatatgaa ggtgatagtc gatatgagta tttatctaaa agaaagacta gttataagta
    26581 ttatgatgtt ggaataatag atttagaagg taaagctgag tttttaagtg gaaaaaagtt
    26641 tgacttaaag aatactgctt cctataaaaa aggaattgaa ggtaaagaat actcagaagt
    26701 aatagagtca atgggttttg ttctttatag tgtacctata tttaacgata atcatgaagt
    26761 gtctagtatt ttaataggtg tatccaataa agaaagcatg gatgagattc ttaatataga
    26821 tagctttaat ggagaaggaa ctattgaaat tattaatagt cagggaaaaa ctcttttcat
    26881 aggaaaaaat aatgagttaa taaaagacct tagctataag tataaggatt ctgcagatga
    26941 accttgggca caaaagatgc ttaatgattt taaaaatgat aaatcaggaa atattacaat
    27001 aacctcatca aaaggtgtac aatgcttatt aacttatcat ccaattacaa gaggtttaaa
    27061 tgattggcat tttcttctca tagtacctga agatgttgta cttggtgaac ttaataagtt
    27121 aaattctttt acaataataa tgacatttat aactacatgt attattggaa ttataacatt
    27181 aattttatat attataagaa gaaaatatgt aaatcaaata gaaaatattg catatttaga
    27241 tagtataaca aatggtataa attcaactaa gtttagtatg ctagtcaaac ctttaatttc
    27301 agaatcacct gatagtactt atatgatgat agcaatgaat ataaaagatt ttaagctaat
    27361 aaatgattgt ttcggtagtg aaaaaggaaa tatgacacta aaacatcttt acaatatttt
    27421 gtataaaaac attgatgaaa atgaagaatt tgtgtgccgt catgatgcag accttttcta
    27481 tttgcttatt aagaatagac cagtgttaga agccctggag ttattatata aaatagaagc
    27541 tgacacaaat tattttaata aaaataggga aaatccatac tttttaagac tatcagtagg
    27601 tatttacaca attgaaaatc ataatgaaga tttaattaca atacaagacc atgcaaatac
    27661 agcaagaaaa agctttaata aaactcatca aagtgatttt tctttttata gtgatataga
    27721 aagaaaacga ttaatatctg aaaaagaaat ttcaaattta atggaaaaag cccttgaaaa
    27781 taaagaattt tttatgtgct tacaacctaa aattgatata aaaagtggaa aaattagtgg
    27841 agcagagtct cttgtgcgtt ggaaaaattc agaaaagggc ataatttatc caagtaattt
    27901 tattcctctt ttcgaaaaaa gtggatttat atgtaagtta gatttatata tactagaaga
    27961 aacatgcaaa ttgatttcta agtggataaa agaaggtaaa gaagtgttga gaatttctgt
    28021 taatgtatca cgtcaacatt tgaatgataa attgttttta gaaaaatata agcatatatg
    28081 caataaatac aatgtaccaa catgtctgat agatttagaa cttaccgaat ctattttctt
    28141 agaaaatcca gaagctatag atataataga ggatattcat tctaatggat ttaaatgttc
    28201 tatagacgat tttggatttg ggtactcatc attagggata ttaaaggatt ttaaagttga
    28261 tataattaag ttagaccgtt cattctttgt tagcaaaaat aatatagacc gtggtaaggt
    28321 agttattaag tctattattg aacttagcaa aaggctaggt atgaaagtaa ctgctgaagg
    28381 aattgaagaa ttagaacaag ttgagttcct aaaaaatgta ggatgtgatt atattcaagg
    28441 ttatgttttt tctaaaccat tattaatttc tgattttgaa aattttgcat atgaaaataa
    28501 taaaattaaa aatatccatt attaaatgta caagataaaa aatttaggca agttatataa
    28561 ttaaaaatat aacttgtctt ttttatttat atatttaata aaaatatatt tgcagatttt
    28621 gtaaaaataa ttttcaaatg ttgtaagttg tggctaaagc tttattgtat tattctgaat
    28681 atagaaagat agttactgtc ttagataaaa actgaaacat acataataat ataaaatttg
    28741 ctatattaat aactctaatt attttggaag gagggaaaat atgataagtg ggagaatgtt
    28801 actcatcatg aattttctaa aaggaaaaaa taagacaagt tacaaggata ttagcaaaca
    28861 acttagtata gaagaaagaa aagttagata tgatataaat aatttaaata ttgttttgcg
    28921 ttcattaaac aagtcagtaa tacaaaagat gaacaaaggt gtgttaatta ttcctgatga
    28981 cttccaagta gtatgtataa ataataacga atatgtattt tccttaactg agcgagtatc
    29041 tattatgaaa attatatcaa tgtttaaaat agataatcta aacttagaaa aattgagtaa
    29101 agaatttcag gtttctagaa gttcaattaa aaatgacctt aatgttttgt caagtgaatt
    29161 agaaagaaac caaataacta ttacttatga taaatctttt aaaataagtg gagaagaaga
    29221 ccttatatat aagcaaagat tgaacatatt aaaatcgtat tcttatcttt ttggtaagga
    29281 aaaatctgat ttaagtgtat ttgagaagta tttagtgaat gtaattgaaa aaatattcaa
    29341 agggatttat ttgattgata tatataattg gtctatgaat ttattattgc aaatgggatg
    29401 gactttaaat gatgaatctt ttacatggta tgtgtcaaac attttccttt ttacatggta
    29461 tatacattca gatgaaaaac atcctttaga atctgcttca ttaagtgagc catgttttga
    29521 aaataagttt gaccaagaat ttgaaacaat tattcaaaaa aagttgacta aggaacaaat
    29581 ttttctgtta aagagttttg tattttttac aaataaatat gcaagtttaa acgaggatat
    29641 ggatttgatt agtacagaaa ttatagttca agacctaatt tacaatatgt cagaaatatt
    29701 agagattagc tttaaagaag atatgattct ttataaagga ttactcaatc atattgcccc
    29761 attgatagag agaatcaagc agaatataca gatttatgag gaattacttc atgtaatacc
    29821 agataaatat caatatgtac ttgaagctac taaggtttct attcaaaaaa acagagtttt
    29881 aagtcaaata aagaatgaaa atgaaataat acttttggca atacatttct taggaagtat
    29941 acatagaaat gagaggaata actacaaaaa tatattgtta gtttgtggat ttggatatgg
    30001 tgtaattgct atgttaaagg atagtttagt aaatgattac cagataaata tagtagagag
    30061 tattccatca tataagttac aagattataa agaatgggaa agtattgact tcattataac
    30121 aacatcaaag atagtaatag acttgcctaa aacaattatt gagataaatc catttttaca
    30181 agaagaggat tatttgaagt ttgaagaaaa agggataaaa agaaagaacg tcttaactaa
    30241 ttatatgtca ataaaaaaaa ggttggactt tttagaagaa gatgctcaaa aaagagtatt
    30301 aagtattata tgtcaagaac ttggatacag tgatgagaga atattattta gacaacttaa
    30361 tttaagtgat ttaataggga ttgataccat aaggataatt gatgaagata tgaaatggga
    30421 agatgctgta aaaattagtt caaacatact tgctaattgt gattttgtat cagcacaata
    30481 tgctgagaat attatagata ttttaaaaaa tgtgggtttt tatgctgtaa aagataacga
    30541 atttgcttta ctacatggca atgatagttc attagtcaaa gtaagttcga taagtttatt
    30601 aatatgtaaa gaagcagtta gttttggaga taaaaaggtt aaaatcatat tttgtctagc
    30661 aagcagagat aaaaaagagc aaattcctgc aataattaat ctaactaaaa tggtttataa
    30721 gactaatttt attgctatgt tagaaagtgc aaagacaaaa gaagaagcag aagttttaat
    30781 tcataaatat gagaaggagg tcactgtatg aataaattag ttaatgtaaa atgttcattt
    30841 aaaatacatg ctaattcaaa agaagaagct attgtatcgt tagttgatgt agtatcaaaa
    30901 gaaggatatc taatagataa aaatcaattt ttggaagatg ttttaaagag agaagaaaca
    30961 ttgtcaactt atattggaca tggtataggt cttcctcata gtcaaagtgt aggtgttaaa
    31021 aactcttgta ttacaatagg aaagcttgat attcctattg aatggacaga agaaggagaa
    31081 aaagtggatt taatattttt aatatcagta acaaaagaca atgaaaataa tcttcattta
    31141 aagattttat caaaattagc acgtttattg atgcatgaaa gttttagaaa tcaaattaga
    31201 tgttcagatg aacaaactgt ttataatctt ataaaagaaa aaatagagga ggaagattaa
    31261 aatgagaatt ttaaagtttt tgcaaaaaca tcttatgaca gcaacatctt atatgattcc
    31321 atttgtggtt gcaggaggaa tattatttgc tttatcagta actctaagtg gacaagctgc
    31381 tgttcctgaa acaggctggt tggctaaact gaatcaaatt ggagctgctg gcttggcttt
    31441 attcattcct atattaggtg gatatatagc atttagtatg gcagataaac caggattagc
    31501 tccaggtatg ataggtgcat atttagcaaa agaagtaaat gcaggtttta taggtggtat
    31561 catagcagga tttatagcag gatttgttgt tttacaatta aagaaaatta aattagctcc
    31621 tacaatgaga actttaggtt ctatatttat ttatcctttg attggtacat tgattacagg
    31681 tggtataatt gtatttttaa ttggagaacc tatagcttct tttatgacat ggatgacaaa
    31741 ctggttaaat ggaatgtctg gtgtttctaa aattccttta ggtggaatct taggaggaat
    31801 gatagcctca gatatgggtg gtcctataaa taaggttgct gctacttttg cccaaactca
    31861 agttgacaca cttccatatt taatgggtgg agttggcgtt gcaatttgta tacctccaat
    31921 tggtttagga ttagctacat tattgtttcc aaaaaaattt agcaaggaag aaagagattc
    31981 aggtaaagct tcgctattaa tgggatgtgt tggaattaca gaaggagcta ttccatttgc
    32041 aacatcagat ccagttagag taataccttg tattatggct gggtcgatta taggaaatat
    32101 tatggctttt ttattaggat gtttaaatca tgcaccctgg ggaggtctga tagtattacc
    32161 tgtagttgac aataggttgg ggtatattgc atcagtatta actggagcag tagttgttgc
    32221 agtattaatg aaattagtaa aaaaagacgt gaaagaagat gaagaaattg aagaggattt
    32281 agatgaatca attgaattaa tatttgaaga attataaata taaatggaag gaatttgtat
    32341 aaaaaagtaa atataaaact ataaacttaa aggagataag aatatgaaaa ttgtagcagt
    32401 aacatcttgt cctagtggag tagcacatac ttacatgtcg gctgaatcac ttgaattatc
    32461 ggccaaaaaa tttggtatag aaataaaggt tgaaactcaa ggaagctctg gaatagataa
    32521 tgaattgtca cttaaagata ttgatgaagc aacttgtgta atattgacta atgatgttga
    32581 gattagaaat atggaacgtt ttaagggaaa gaaggtagta agaatgagtg tatctgagat
    32641 aattaagaaa tctgatgcat taattaagaa aataaaagat acttttcaat aaaaattgat
    32701 ttattttaca tataactcaa agatgttttt aaagaaattt ttattaaaag catctttgaa
    32761 aatatcttaa aaatagaata aaaggagata tagtcatgaa aaaattacca ataaagactg
    32821 tcgtacaaag attactagac ttacaagaag aggggaaaag tgcaactctt ttaggaattg
    32881 gtcctatgtc accaaacttg ttacaagcaa gttttgaact agcaaaagat gatgattttc
    32941 ctttaatgtt tatcgcaagt agaaatcaag tagatgcaga tgaactaggt ggcggttatg
    33001 taaatggttg gaatcaggaa acttttacaa aagatattaa gaagattgcc gacaaagttg
    33061 gatttgatgg tttatattat ttatgtagag accatggtgg gccatggcaa agagataaag
    33121 agagaaatga ccatttgtct gttgatgaag caatggagtt aggaaaaaaa tcttatttag
    33181 cggacattga agcaggtttt gacctattga tgattgaccc tacaaaagac ccatttgaaa
    33241 ttggaaaagt aattccacta gatgtagtac tagaaagaac tgtagaatta attgagtatt
    33301 gtgaaaacga gagaaaaaga cttaatctac cagacatagg ttatgaggtt ggtacagagg
    33361 aaaccaatgg aggtcttact tcaactgaga cgtatgaaac atttataact agactaaagg
    33421 ttgaacttga taataggggt ttgccaatgc caacatttat agttggtcaa actggtactt
    33481 taactagaaa gacagaacaa gtaggaactt ttaattttag aaatgcttat gatttagctc
    33541 aaatggctaa gaaatatgga gttggtttaa aagagcataa tggcgattac ttagatgatg
    33601 taactttatt agaacatata ccatctcaaa ttattgctac aaatgtagct cctcaatatg
    33661 gtactgaaga aactagggca tatttaaaat tagcagaagt tgagtgtaaa ttagaaaaag
    33721 agggcctagt tgataaaact tctaatatca gacatgttct tcttgttaat gcaattgaat
    33781 gtggacgttg gagaaaatgg gtagtaggag aacaaaaaaa tctaactact gaagagatat
    33841 ttaagaatga agttttatct aatgaaatac tagatattgc aggacattat acatttaata
    33901 atgatgatgt taaaaaagaa atagaagtgc tttatgataa tttaagtaag aataatattg
    33961 atggtcaaag atttgttgta gaccatatta aacgtccttt gcgtgattat gcagaatgct
    34021 ataacttaaa aggtgttact acgaggattt taaataaata ataggactta tagtggtgag
    34081 tattgttata ctaggtattt atagttataa ttatacttac cactattaaa tttaggagga
    34141 ctaatatttt gattaattta atttttgatg tagatgatac tttatataac caattaacgc
    34201 ctttttacac tgcatataat aaagtgtttt cttcaataaa ggatatttct attgaagatt
    34261 tatatatgag tagtagaaag tatagtgatg aggtatttca tatgactgaa aatggagaaa
    34321 tgccaatcaa agaaatgcat atttatagga taatgaaagc ttttgaggag ttgggaaatt
    34381 ctattacaga aaaagatgca caaagttttc aagatgagta tatatatcag caatcacaaa
    34441 ttacactaat accagaagtt gagaggattt taaatttttc taaagaaaga aatattaatc
    34501 taggaattat aactaatggc ccatcaaacc atcaaagaat gaagttaaag cagttaaata
    34561 ttgaaaattg ggttgataaa agtaatattt ttatttctag tgaggttgga ttttctaagc
    34621 cagatactaa tatttttaga gtagctgaga atgttatgaa tttagataga gaaaatacat
    34681 attatgtggg tgattcttat agaaatgatg tattaggtgc taaaaaagct ggttggaaga
    34741 gtatatggtt gaaccataga ggacatgagg tagaagagtt gttttataaa cctgattttg
    34801 taattttgga acataaggat ttgatatctt tatttataaa aatatgtagt tataaaaata
    34861 tgtagtaaaa aaatgttaat aatagaattg tataaaataa ttaataataa ctatataaaa
    34921 ttatatgttt agattgtgag caatctattt tatgttaaaa atagattatt tagaagaagg
    34981 ggaaatctta aaaaaatttg aatatcccct ttttattatt taattaaaat gaaatagtta
    35041 gaaatatatt ttatttagtt tagtcaataa cattttggga ttctttataa ctacatgtta
    35101 actgttgaat aggcttatca tatggtttaa cccatccttt attaatactt aattctgtaa
    35161 gtgctgtgtg accttctacc atttgagtta gtgaagctga atatatagct cttaattcag
    35221 gtgtactact tgttaatgca gaattaagat acatattggc agatgcttta gcagatgaaa
    35281 gcatacttaa cattattact ttatcgtcta tataagtatt gttcttaatt agattttcta
    35341 tcatatttct catgttatct atacctcctc agaaattagt atttcatttt caactataaa
    35401 ttgttgtata gctttaattc ttccttctgt tgctaatata ctagattctg attgtctttt
    35461 taggtcttca tctgatatta acatatttat tgctctttgc ataagtaatc cgtcagactc
    35521 catttttata actgctgcta aagatatttt ttcagtttct gaaagtgttc tcatttaatc
    35581 atctcctata ttgtaattta tgctttaata gtatgtcttt cttttcaagg aaataaccat
    35641 tacacttttt tacagtattt tatattctct ttatatcaaa gtttagtcaa cttaatatat
    35701 taatataatt ttgtaggata tttatcatta aatatctata aaataaatta tttgaatatt
    35761 cataaatcaa aatttatttg tatgtacttc ttcaagagtc aatatgaatt ttttcaaaaa
    35821 attaattttg atgataatca ttaagggcta tatttttcct aaagttgtga ttatcattat
    35881 taatttacta aaatacttta atgaaaaata gctaacttt
//
LOCUS       DAELXX010000029        34901 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.029, whole genome shotgun sequence.
ACCESSION   DAELXX010000029 DAELXX010000000
VERSION     DAELXX010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34901
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.029"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(64..264)
                     /locus_tag="KPH29_001382"
     CDS             complement(64..264)
                     /locus_tag="KPH29_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1467004.1"
                     /translation="MPKFICNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
     gene            complement(266..616)
                     /locus_tag="KPH29_001383"
     CDS             complement(266..616)
                     /locus_tag="KPH29_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HBG1467005.1"
                     /translation="MIQKKIFMGFCGFLGFLSLRYFSSGNVTDLTYIGFFAFFSNFII
                     AKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNVELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            778..966
                     /locus_tag="KPH29_001384"
     CDS             778..966
                     /locus_tag="KPH29_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG1467006.1"
                     /translation="MTKYDFNFKLKVAKFYLNREGRDILIVKQYGVYDHSQVERWVNI
                     LGEGALKIKKFSILYGSS"
     gene            complement(1442..1592)
                     /locus_tag="KPH29_001385"
                     /pseudo
     CDS             complement(1442..1592)
                     /locus_tag="KPH29_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897489.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1989..2483)
                     /locus_tag="KPH29_001386"
     CDS             complement(1989..2483)
                     /locus_tag="KPH29_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG1467007.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIIALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTDGGMISWVKKDGKIIINGGFKDDPLADKIENLGMPYNG
                     SYLLMSSPGHEKYLELSILPEGEISKSGLDKLKNDYGNLIDITNDQNKINIVIKLLNN
                     KSNT"
     gene            3289..3666
                     /locus_tag="KPH29_001387"
     CDS             3289..3666
                     /locus_tag="KPH29_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG1467008.1"
                     /translation="MKEEILIEKLLRAELIVMEYLCKKDTLMPKKEIVKEIKQIYGWH
                     KSTIKILLKRLVAKRYLARYIINFQYHYKIIIDNKEYYTFKKKVLKSSKSIKIMRSLT
                     TTHKNVSKEKLDLLDEYYRNLKE"
     gene            3771..4146
                     /locus_tag="KPH29_001388"
                     /pseudo
     CDS             3771..4146
                     /locus_tag="KPH29_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     gene            4758..5450
                     /locus_tag="KPH29_001389"
     CDS             4758..5450
                     /locus_tag="KPH29_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1467009.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            5646..7706
                     /locus_tag="KPH29_001390"
     CDS             5646..7706
                     /locus_tag="KPH29_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBG1467010.1"
                     /translation="MEKYRALKLVSLIIISISFPLTILLATIGLAEPKYNIQKTSTKS
                     GIFNISNDFYDSNIFDEGTVESLLSNFKISVDSQLTSEEKSKLQTKSKSIDLSSNDSE
                     YDKIMINKQASEEYLSYLKNIKVYVINTYTGVFYTNTSYGNLDDFKQKTKSYCNIEII
                     NNVGKSSYIKTINGEKFELNNLSMELNGVDEAFEAYVSFPEEPTIEDGIVYTNFQIFK
                     QATEKVRLFFVACLSLAIVCLLSIILYSRIKGEVLKRNNVFLLIYSKIPMEFNVILVF
                     ISIAILTMYALAVSYHIDVIVLVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSIIVR
                     VILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMVISTRSIGMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYIKVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     YIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNFDPS
                     EIMERFKRADESRNTEGSGLGLAIARDLVALQGGKFYIEIDGDLFKANLEFQKYEEEI
                     TNME"
     gene            complement(7912..8235)
                     /locus_tag="KPH29_001391"
     CDS             complement(7912..8235)
                     /locus_tag="KPH29_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBG1467011.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
     gene            complement(8228..8929)
                     /locus_tag="KPH29_001392"
     CDS             complement(8228..8929)
                     /locus_tag="KPH29_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBG1467012.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
     gene            complement(9207..10592)
                     /locus_tag="KPH29_001393"
                     /pseudo
     CDS             complement(9207..10592)
                     /locus_tag="KPH29_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
     gene            complement(10700..11938)
                     /locus_tag="KPH29_001394"
     CDS             complement(10700..11938)
                     /locus_tag="KPH29_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG1467013.1"
                     /translation="MELFIEVIKFNIYISLLILFFILFTKKISKYTFKFNYFICLLIV
                     VRMMFISKINLNILNINNTTINIYKHNLDNYIPYDQGITLDIFMYIWILGIIAISFYY
                     LRTNFKFYNYIENFKKEILEIDITSILDEQAKELSINKKLQIYKVKGIYSPAIIGLTR
                     CKIIIPEKKYNNKELRFIFKHELIHYKRKDNLFKLLISIICIIHWFNPILYLLKKYFH
                     ELCELSCDEIVIDKCTTSEIKEYAYIILNTIKYQKTLKYSSFVSQFLNKKNIILKKRL
                     NSMFNQDKKKINLGLKFLLTFIIIGSIFSINSNFKQNEDVEYTIAKINYNKNGETYGT
                     HIIDSNGNSIEPELIQTIGRGKKVGYVKKRDLYDCDNQPKTPEEAVLYTRKRNFNIFK
                     KTIPLYDKEGIKIIGRFEIN"
     gene            complement(11951..12319)
                     /locus_tag="KPH29_001395"
     CDS             complement(11951..12319)
                     /locus_tag="KPH29_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG1467014.1"
                     /translation="MKKIPKSELLVMNFIWDKGIDKIPAREVANYMEISHGWTKGTTG
                     KVLSRLSDKGFLEFEKDGRNTLYTIKVKHEEYLKFETKDFLELIHKNSIHSFISSLGC
                     GNEISDDDIKELEQWLKKRK"
     gene            complement(12847..13269)
                     /locus_tag="KPH29_001396"
     CDS             complement(12847..13269)
                     /locus_tag="KPH29_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBG1467015.1"
                     /translation="MNTFKTEVLLKYSAKDIFNIFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESTITRYELVHIDLENTKLIFIESESKRNIF
                     GKINSALVRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
     gene            13747..16650
                     /locus_tag="KPH29_001397"
     CDS             13747..16650
                     /locus_tag="KPH29_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG1467016.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISISEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEESEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILVL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     ISIKIPDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIVTLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDENLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPVKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            16683..17129
                     /locus_tag="KPH29_001398"
     CDS             16683..17129
                     /locus_tag="KPH29_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG1467017.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGEMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            17252..17554
                     /locus_tag="KPH29_001399"
     CDS             17252..17554
                     /locus_tag="KPH29_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG1467018.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            17735..19141
                     /locus_tag="KPH29_001400"
     CDS             17735..19141
                     /locus_tag="KPH29_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBG1467019.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPIITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFSDPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVISMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            19485..20543
                     /locus_tag="KPH29_001401"
     CDS             19485..20543
                     /locus_tag="KPH29_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG1467020.1"
                     /translation="MGDKMRASVLYNVGDVRYEMVDIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYASPYPGRAWTATLDFMGKGDIIFKPMISHKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            20618..21304
                     /gene="araD"
                     /locus_tag="KPH29_001402"
     CDS             20618..21304
                     /gene="araD"
                     /locus_tag="KPH29_001402"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBG1467021.1"
                     /translation="MLEILKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNVVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEICNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            21322..21969
                     /locus_tag="KPH29_001403"
     CDS             21322..21969
                     /locus_tag="KPH29_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBG1467022.1"
                     /translation="MLDSLKGKVVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GIGREELNVEHICVLDLNGNVIEVEKGIKPSSEVLMHIEIYKIRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIAPYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFESWKYPEEIKF"
     gene            complement(22142..23509)
                     /locus_tag="KPH29_001404"
     CDS             complement(22142..23509)
                     /locus_tag="KPH29_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HBG1467023.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLRSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(23699..23787)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            24215..26335
                     /locus_tag="KPH29_001405"
     CDS             24215..26335
                     /locus_tag="KPH29_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBG1467024.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKPIKRLWISSSTDKAIKDGFAKLRSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESILKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKICNKLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNVLEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKEIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRIFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            26439..27692
                     /locus_tag="KPH29_001406"
     CDS             26439..27692
                     /locus_tag="KPH29_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HBG1467025.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFEDIETYSKDSQKKREKEL
                     DKFINTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEIYYSKANFAYSMLYPYTD
                     NYLDNTNIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSQMIDYIESVYPREKFTQV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYLISGFMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMSKEGTLDKVVNKLINFTIELIDSFK
                     INNKNKSVITMIKNDCLMLILFSVVYNAEFFSVGYIKEVEKFIPYTIDYSLEIEEKIK
                     EKFKNIDVLNNENEYKEMIDIICAE"
     gene            complement(27846..28907)
                     /gene="ytvI"
                     /locus_tag="KPH29_001407"
     CDS             complement(27846..28907)
                     /gene="ytvI"
                     /locus_tag="KPH29_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBG1467026.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLIFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLSLILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFLELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFIGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
     gene            29181..29921
                     /locus_tag="KPH29_001408"
     CDS             29181..29921
                     /locus_tag="KPH29_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HBG1467027.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILKELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            29933..31711
                     /locus_tag="KPH29_001409"
     CDS             29933..31711
                     /locus_tag="KPH29_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG1467028.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGIALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRILEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIKERKLLIKKLLLDILNENGLVLTDIEFKSVLNNILVMLS
                     RHKYNEKEFIENYIENYRSKRSSILENEQNKEIIINSIKTFCKNLKLATSIDISNDKV
                     FEEFLYKHISSLCKKIKLGINQSPIMAQDIKIKYPFAFELANIAKKTIEKDLKIEINE
                     DEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLIKAKLENIFKEKLEILK
                     IIPSYLVEYIKVIDVDFVISTVPIEVGDVPVINISPILSEKEIRLIEKYIETGNIYLD
                     LDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDMKKSYFEREKIATTEIG
                     NMVTIPHGAIGKIFDNKIAIGILKKPISWEISDVRLIIMLALDKDKILDYEVIFSKIY
                     KRVDSIAKVISICENSSFEKFVNLFN"
     gene            31878..32795
                     /gene="pfkB"
                     /locus_tag="KPH29_001410"
     CDS             31878..32795
                     /gene="pfkB"
                     /locus_tag="KPH29_001410"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBG1467029.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     ILKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYAKRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKQF"
     gene            32832..34751
                     /locus_tag="KPH29_001411"
     CDS             32832..34751
                     /locus_tag="KPH29_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG1467030.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVAEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIVLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
BASE COUNT        13859 a         3839 c         5486 g        11717 t
ORIGIN      
        1 caggagtgac ttaaagattg aatttaagtt tgtatttaaa acacattttt aataatgcaa
       61 aaactaatca aaaataaaca attcatcaat aggtgtatct actatttttg atatatctat
      121 tgctaatttc aaagaaggat tatactttcc tgcttctaaa cgcataatgg tttctcttct
      181 aactcctact ttttctgcta attgttcttg agttaattct tttaattgtc tatatttttt
      241 taggttacaa atgaatttgg gcatattatt tttcctctaa aatataaagt tttatagcat
      301 aaacatttaa agtgattgca tatgtaattg atagtaaaac acacattaat tctacattcc
      361 tagacactat agtaatacac cataaaatga ataattctat aatagcaagc tgtcctgtaa
      421 atgccatagc tgctttttca tcttgtacat acctttcatc tgctttatca ccatttattt
      481 ttgctataat aaagtttgag aaaaaagcaa aaaatccaat atatgtaaga tcagtaacat
      541 tgccagatga aaaatatcta agacttaaaa atccaaggaa accacaaaaa cccataaaaa
      601 tttttttttg aatcattttt tatcctcctt agtaatttta aaagagatat atttgtatct
      661 ttataataca aatatatctc ttttatattc ttttgtcaat agaaaatcag tattatacaa
      721 tatttgtact gcaccataat tgttagagta aaaatctaac gcttgagatg tttatgtatg
      781 actaaatatg attttaattt caaattaaaa gtagctaaat tttatttgaa tagagaagga
      841 agagatattt taatagtaaa acaatatgga gtttatgacc attctcaagt tgaaagatgg
      901 gttaatatat taggagaggg tgcactaaaa ataaaaaagt tttctatact atatggttca
      961 agttagatgt tataaactat atgatgataa ctaagtatag tgtacaataa atcgctaagt
     1021 attttggaat aaacaatagc acagttatag caacatgaaa acaaaaattt ttacatagtg
     1081 gtgttgatgc ctttttcaaa atgaaaggaa gaccataatg aataaaaaca aaaacactaa
     1141 agaaaaaata aattaacaag agaacaagaa ttagaatatg aaaataaatt tttaaaagat
     1201 gagcttgagt ttttaaaaaa gatgtgtgcc tcagggaagc cacattaaaa atagtggtat
     1261 cattattaat caaaagaaag ttagatgtat tatcaaaaaa ttagaactta aatgcatagc
     1321 attttttcat aaatcacata aatataactc ctataaaggt acaattggaa aagtaaaaaa
     1381 taataaaata aatagaagat ttaaaacatc aatagcatat cagaaaataa caaatgatat
     1441 gctagaattt ataaaatata tctaattata aaaaaggcat atctcgattc attttttgtt
     1501 atattgtcaa ataagaatac tatagatgaa agatacgatt ttgcagatgt attaagtaag
     1561 tattctgatg tttttggttg atacacatgc aataaagaag ttgtgtttgc agataaatta
     1621 gcaaaactat agtataagtt gcaacaataa agagaatgaa aattatcata ctaaagcata
     1681 agacatgtta caattttatt agataggaca ttaacataca ctaaagtact ttttattata
     1741 aagagtgctt atttttttga aattcattaa tgtataaact atcaagaata ttactcaaca
     1801 caatttaaaa acaacttaaa taggtatttt tagcaacata aattatgtga tataataaaa
     1861 aatagaagtg tatgccaata cacttctgta gttataacta agcattctat tcttggggag
     1921 tgcttttttc atttctccaa acaagattgt ttatttcatc agtaattatt aaatcaaatt
     1981 tattgtattt aagtattcga tttattattt aaaagtttta ttacaatatt tattttattt
     2041 tgatcgttcg ttatgtctat taaatttcca taatcatttt ttaatttatc tagaccactt
     2101 ttgcttattt ctccttctgg aagtatgctt aattctagat atttttcatg accaggagat
     2161 gacattaaca gatatgaacc attataaggc atccctaaat tttctatttt atctgctaat
     2221 gggtcatctt taaaaccccc atttattatt atttttccat ctttctttac ccatgatatc
     2281 attcctccat cagtatactt ggattcatca gcatagtaac taagagatgc actttttatt
     2341 gcacttatat cagcttcaag tttagctatc tttgcttttt ctatattttt aaataatgct
     2401 ggaagtgcta ttattgccaa tattcctatt atagcaatta ctactaataa ttccactaga
     2461 gtaaatcctt ttttattttt catttatttc ccctccatat aaaattaata atatataaat
     2521 ttttattttg ttgatttcta ctttctatat tttaccatat atagacaaaa aaatcacttt
     2581 ctatgacaaa tatactatta ttttacaatt tatagaaatg atttaaaatg acatttcttg
     2641 ttagaaaaga tataagcaat ggattaaaat aaagaaaact acagtcatat caatgtcaat
     2701 aatattattt aaaagcagat gaaaataaaa agaggaccca gaaaataatg tctatttaat
     2761 tatacttact taccaatacc ataacttgat ataaacaaga ttatactaca ttttttttat
     2821 gctctattat aaaatcatct aactgtgtat ctataaaatc aaaataatta tatgaattat
     2881 cagtataata agtcaaaata tgtatacatg aatacatatt tttaaaagat atatattatt
     2941 acatacctaa aaaactttca taaaaacaaa aataaaatat gcttttagta acttgttaac
     3001 ataacttata atttatttta taagcattag cctatatagt tttgtgctac taaatttctt
     3061 tgattctaac gatttagtgc tattctacaa ctaaaataat acaatagagc tatttattgt
     3121 ttggtagcat tgtttgcaaa ttaaacaaat atatatatat gaatattatt ttacgtttaa
     3181 aaaaacagac taaatatgta gaatatttgg taaaacttac ctctagtttt tgtcttaaaa
     3241 agctcctatt attaatttaa gttagaagaa ttaaggaagt gacataaatt gaaagaagaa
     3301 atattaatag aaaaattatt aagagcagaa ttaatagtaa tggagtattt atgtaaaaaa
     3361 gacactctaa tgcctaaaaa agaaattgta aaagaaataa aacagattta tggatggcat
     3421 aaaagtacaa taaaaatact actaaaaagg ctagttgcta aaaggtattt ggctagatat
     3481 attataaatt ttcaatatca ttataaaata ataatagata ataaagaata ttatactttt
     3541 aagaaaaagg tactaaaatc tagtaaaagc ataaaaataa tgcgttcact taccactaca
     3601 cataaaaatg taagtaaaga gaagttagac ctattagatg agtattatag aaatttaaaa
     3661 gaatgatata gtaaaaatcg aatgtaatta tttaccttaa ttcttttgtt tgtaaaactt
     3721 tattgatata gaatttaaca tatacataaa ttggtaaagg gatgtatact atgaaaatta
     3781 gtaaattacc agaagcggaa ttaaaagtta tgagatatat ttgggaatca gaaaaggtat
     3841 taatatcaag agaaatcacc aagtctatgg aacaaaaata tgaatggaaa gatacaacta
     3901 tatttacagt attaaaaaga ttactaaaga agagagtttt tagaaacgga gaaaatagat
     3961 aagcgtacac actataatgt attagtaaaa gaaaaataat atcagagatt ttaaacaaaa
     4021 gaatttttaa agaatacaca taaaaattca atattaagtt taatgtcagc actatgtagt
     4081 aaggatgatg aagtagatgg tgatatttta aatttattag aagaatattt taagaattta
     4141 aaataagaaa atgacagcta gatacataat aaataaaatg aaggtatgaa attataactt
     4201 tttctacatt cattttgttt attattctga acatgttaaa aaaatgaaat aagtttgtat
     4261 tttatttata taaattttaa tagaatatgt acagtatata gtagatagaa tgtataaaaa
     4321 tagaatttaa ataagtagct attattgcag tgaaataaat gtttctaagt atatttgggt
     4381 ggaagaatgg tggaagaaaa ttagtgaaat gtaattcttc caccattctt ccaccccaaa
     4441 aataaaaact gttcaataag aattaatata cattaattca gctgaatcca atcaaaatac
     4501 ttaaataaaa gtaatctgaa aataacataa ttcaaatcaa tttattcaaa taaatctcaa
     4561 tttatcttaa atcttaagaa atatttaaga attaggataa tattttctta agatttatat
     4621 tttattatat agttataagg gaaagttaca aaaatgtaag ccaaagtaat ataacaattt
     4681 agtcattgtt gagaaagtaa gtgaaagata ttgtataata tagataagta ttattataca
     4741 atagaggaag tgaaaatatg tacaatatat tagtagtaga cgatgataaa gaaattgttg
     4801 attcaataga aatatatctt aaatcagaag gatttaaaat atataaagca tatgatggaa
     4861 tgaaagcttt ggaaataata acagagaatg acattcatct aatactaatg gatataatga
     4921 tgcctaaatt agatggaatc aaagcaactg taaaaatacg agaagaaaga aatattccaa
     4981 taattttaat ttcagcaaaa agtgaagata cagataaaat aataggatta aatattggag
     5041 ctgatgatta tataacaaag ccatttaatc tattagagct tatagctagg gttaaatcaa
     5101 acttaagacg atatgtaact ttaggtaatt atgaagatac atgtaaagaa attttaaaaa
     5161 gtggagcttt ggagttaaat acactaacaa aagaagttaa aattgatggt gaaaacataa
     5221 aaatgacccc aattgagtat aaaataattg aacttctttt agaaaataaa ggacgagttt
     5281 tctcaataga tgagatatac gaaaaagtat ggaatgagga aagttttaat tcagaaaata
     5341 cagtagcagt acatatcaga cgaataagag aaaagattga aataaatcca aaagaaccaa
     5401 gatttttaaa ggtggtgtgg ggagttggtt ataagattga aaaattttag tggaaaaatt
     5461 ataaaagaaa agagtttaat atttaattgc attttgattt tagtcatttt tacatttaca
     5521 caaatgttag gaattttaat atgttttaat tttttctctg agagtttaat ttactctatg
     5581 gggattatgc ttttcttaaa ctttatttta tataaaatac tgacaatgta gatgaggtgg
     5641 gcaacttgga aaaatataga gcattaaaat tagtttcatt aatcataata tctatctcat
     5701 ttccattaac tatattgttg gctacaatag gacttgctga gcctaaatac aatatacaaa
     5761 aaacttctac aaagagtggg atttttaata tatctaatga tttttatgat agtaatatat
     5821 ttgatgaagg aacagtggaa agcttattat ctaattttaa gatatctgtg gatagtcagt
     5881 taactagtga agaaaaaagt aaactccaaa caaaaagtaa aagtatagat ttaagtagta
     5941 atgattctga gtatgataaa atcatgatta ataaacaagc ttctgaagaa tatttaagtt
     6001 atctaaaaaa tataaaagtg tatgttatta atacatatac aggagtattt tatacaaata
     6061 catcatatgg taatttagat gattttaaac aaaaaactaa gagctattgt aatatagaga
     6121 taataaataa tgtaggaaag agctcgtata ttaaaacaat aaatggagaa aaatttgaac
     6181 tgaataatct gagtatggaa ctaaatggag tagatgaagc ctttgaggca tatgtctctt
     6241 ttccagaaga gccaactata gaagatggaa tagtatacac taattttcaa atatttaaac
     6301 aagctacaga aaaagtaaga ttattttttg tagcttgttt aagtttagca attgtgtgtt
     6361 tattatctat cattttatat agcagaataa aaggtgaagt tttaaaaaga aataatgtat
     6421 ttttactaat atacagtaag atacctatgg aatttaatgt aatactagta tttatttcaa
     6481 ttgcaatttt aactatgtat gctcttgctg tatcttatca tattgatgta attgtattag
     6541 tttctttatc tttactgata gtatcaaata atatattttt tataaatgtg caattgagtt
     6601 atttagaaaa taaaatgtgt atatttaaga caagtattat agtaagagtg attttatggt
     6661 taataaaaac tgtaagggag gtcagtaacg ctataaagaa agtatcttta gcaaggaaag
     6721 ttattgttat agtgataata tctttagctt taatcatggt aattagcact cgaagtatag
     6781 gtatgggatt actgttttct atatgtattt ttttaggatt tacaatatat ataactaaaa
     6841 gactttcata tcttagttac gtaatagaag gtacagaaag aataaaaaag ggagaattag
     6901 attataagat aaaaattact gggaatgata attttacgtc tttagctgaa aatataaata
     6961 atattggaga aggtcttgat agagctatag aagagcaagt aaaaagtgaa agaatgaagt
     7021 ctgatcttat aacaaatgta tctcatgatt tgaaaactcc acttacttct ataataaatt
     7081 atgtagattt aattaaaaaa gaagaatcaa tacaaccaga gtatataaat gattatataa
     7141 aggtacttga gtcaaagtca aaaagactaa aattattgat agaagattta tttgaagcga
     7201 gtagagtaag tagtggcaat atagaattac aaattagcaa gattgattta gtgcaactat
     7261 taagacaaag cataggtgaa cttgaagaaa aattatctaa aaataattta tatttaaaat
     7321 taaatgttcc aaatgataag gtgtatatat gggcagatgg aagaagaatg tatagagtat
     7381 ttgaaaatct tttatctaat atagcaaaat attccttaga agaaactaga gtctatattg
     7441 atgtatatga ctatggtgaa aatgtaaagg taactatgaa aaatatatct tcttatgaat
     7501 taaattttga cccaagtgag attatggaaa gatttaaaag agctgatgaa tcaaggaata
     7561 ctgaaggaag tggattaggg cttgctattg caagagattt agtagcttta caaggtggaa
     7621 aattttatat agaaatagat ggagatttat ttaaagcaaa tttagagttt caaaaatatg
     7681 aagaagaaat aacaaatatg gaatgagata gtatttaagt atatatattt tttttactgt
     7741 cgaaagcttt tattataata tattagaata tttacagtag agaatgaaga gtaaaaacag
     7801 agtaacttta agtcactctg ttttttattt aaaatcaata atataacaac ttaaattaaa
     7861 attgtacaca tagttttaaa ataacttata caataaaaca ttatccaaat atcacaagat
     7921 aattccaaat aaatatatag atatcactcc tgtaataata gaaataccaa tagatttaaa
     7981 cttatatgca acaagaagta caatcataaa agatataatt ttagggttag ctatagataa
     8041 atatagacta tctccattaa aaaatacatc tttagcagtt aaggaagtaa gtattgctac
     8101 aggtataaat ttcatgattt ttttcaaaaa atcaggcatc tctttttttg aaaaaattaa
     8161 cataggaagc actcttggta gatatgtaac aatgtacatt ccaattataa taaaaaatat
     8221 ataattatta ttcataagat tcctcctttt tacagtaaga ttcttctata aagaaaccaa
     8281 ttaaagatgc taaaattgat gcaactatta ttactaaatt gcttttgaat aatgcatata
     8341 aaataacaga aataatgatt gatgatataa atactacaat ataaatctta ttttttattt
     8401 gagaaactat taaagctata aacatagaag tcaatatgaa gccagaaata gaactgtcta
     8461 tagatatact ttctcctaaa aatgctccaa taaaattagc taaaactgat gaaattaagc
     8521 agaagaaatt aagataaata gcatttctat cagaccaatt tccagcagta aatttttcat
     8581 aattaactgc aaaagtttca tctgttatag tatgacaaaa tggcaatata aattttggac
     8641 ttttattttt tatgtacatg ttcaaattag aactcattag aaaatgtctt aagttaacta
     8701 taaatgtagt caaaacaata gatgttacac ttgcaccagt agaaatcatt gaagctgcaa
     8761 tgaattgaga acttcctcca aatactaaag ttaccattag tgctatttga aaaggtgaaa
     8821 gaccagattt ttgtaatagt attcctccta ctatacctat aggtatataa cctaagaata
     8881 taggaattga agcaataaag ctttctttta attgttgagt tctttccaca ataaatcact
     8941 ccttttgtat taaatgtatt aaagtattag taaaacagaa aaatagatta attgtataat
     9001 tgcaattata attaaaaata aatttggatt aatatataaa atactataca acaaataaat
     9061 aatcttaaac ggttattaaa ttttatctat ttgttattat gatgtaataa tacatattaa
     9121 tctctataag ttaattacta tctggtgtaa ttttatttta acaacaaaaa aaaatacaca
     9181 caattaaaat attgtgtgca atacaattat tttttctctt ctaatagtct ttttaatacg
     9241 ctcagaccag ctttaagttc ttctgtgctt tctgtagcac atacagctat tcttattgca
     9301 gaaattggtt tttcttttcc aacagcaaaa cgttcagaag catagacctg tacaccttct
     9361 ttaagtgctt gtatctcaaa tttttctgcc attattccat caggtagaat caaccatcta
     9421 aatatacatt cttcatttcc caaaagatta tatccagata aatattgatt aataatcttg
     9481 tttcttcttc ttgcagattt acggcgtgca ttaattaatt tatcggcttc acctgatgta
     9541 atcatgcgat aagcaatttc tgttaaaaaa taagattgag atagattaat gttgtataat
     9601 gcgcttgata atgcctctcg atactgttta ggtgtagaga tatatgctag ccgtaaacta
     9661 ggtgcaataa tttttgataa actcgtaata taaattgttt gattgggcag ataggaagcc
     9721 actggattta aatgtgtttc attaagtaaa ctatgaatag catcctcaat aacaattaaa
     9781 ttgtatttag atgcaatatt tgcaatcatt tttcgtccat tttgtgacat tatatgtgta
     9841 gttggatttt gataatctgg tattatgtat agacctttaa tattttcatt tttacaagca
     9901 tataatagtc cttcttcact aatctcatta tcttcttgtt taattggaat tagttgaatc
     9961 ccaagcattt tagctgctgt ctttattcca ggataagtta atgggtctac acctattcta
    10021 tctccatgtt gaaacaaacc agctaaaata gcaacaattg cattttgtcc accacttgca
    10081 ggaagtaagc tatctgatgt agtttcaagc ccagctttat aaattaattt ggcaatagct
    10141 tctttttgcc atttagcacc atctctttgt ccatattgga atatagtttt aaaatctatt
    10201 tacttaatta tatgtttaaa caaagtattc atttcttcta gtgtgaaatc tggattcatt
    10261 gtgcccattt caataaatgt aattttatta ttactggaca tcaagaatag atttgaacgc
    10321 gtatcataag atacaaaagt tccacttcct gtaactccac ttattagacc ttttttttca
    10381 caaattttaa aagctctaga gatagtactc acattaacat ctaagaaatc tgccaattca
    10441 cgttggggtg gtaatttagt acctggtaat aaaaatccat tgttgatatc ttgttctagt
    10501 tgttctgcaa gcgccttata taatatttgc ccttttgaag gtctctttgg tttccagttc
    10561 ataggataat tttcaaaaga attaattggc ataagttttc acctcacatt attaatttga
    10621 ttttttcttt atctaaaaca ataaattcta aagatacaaa taaaaactga taggaaataa
    10681 ttctatcagt ttttgtcttt taattgattt caaatcttcc tataattttt ataccttctt
    10741 tatcataaag agggattgtt tttttaaata tattaaaatt tctttttctt gtatataaaa
    10801 cagcttcttc tggtgtcttt ggttgattat cacaatcgta caaatctctt ttttttacgt
    10861 agccgacttt tttacctcta cctattgttt gtataagttc tggttcaatg gaatttccat
    10921 ttgagtctat tatatgtgta ccataggttt ctccattttt attataatta atcttagcaa
    10981 tagtatattc tacatcttca ttttgtttaa aattggagtt gatagaaaat attgacccta
    11041 ttattataaa ggttaataaa aacttaagac ctaaattaat tttcttttta tcttgattaa
    11101 acatagaatt taacctcttt tttaaaataa tatttttttt atttagaaac tgagatacga
    11161 atgatgaata cttcaaagtt ttttgatact ttattgtatt taatattata tatgcatact
    11221 ctttaatttc actcgtggta catttgtcaa tcacaatttc atcacaagat aattcacata
    11281 attcatgaaa atattttttt aataagtata aaattggatt gaaccaatgt atgatacata
    11341 ttatactaat tagaagctta aataagttat cttttctttt ataatgaatt aattcatgtt
    11401 taaagataaa tcttaactct ttattattgt actttttttc tggaattatt attttacagc
    11461 gtgttaaacc tataatggca ggggaataaa tacctttaac cttatatatc tgaagttttt
    11521 tatttatact tagttctttt gcttgttcat ctaagatact agtaatatct atttctaaaa
    11581 tttctttttt aaaattttct atgtaattat aaaatttaaa atttgttctt aaatagtaaa
    11641 aagagattgc tataattcct aatatccaga tgtacataaa tatatctaaa gtaatgccct
    11701 ggtcatatgg tatataatta tcgagattat gtttatagat attaatagta gtattattga
    11761 tatttaatat atttaaattt attttagata taaacatcat tctgactact attaaaagac
    11821 atataaaata attaaactta aaagtatatt tgcttatctt tttagtaaat aaaatgaaaa
    11881 aaagaattag tagacttatg taaatattaa atttaataac ttctataaat aattccataa
    11941 aatacgtctc ctattttctt ttttttagcc attgttcaag ttcttttatg tcatcatctg
    12001 atatttcatt tccacaacct aatgatgata taaagctatg aatagaattt ttatgtataa
    12061 gctctaaaaa atcctttgtt tcaaatttta gatattcctc atgtttaact ttaatagtgt
    12121 ataaagtatt tctaccatct ttttcaaatt ctaaaaaacc tttatcactt aatctagaaa
    12181 gtactttacc agtagtacct tttgtccatc catgagaaat ttccatataa ttagctactt
    12241 ctctggctgg tattttatct atgcctttat cccatatgaa attcataact aacaactcgg
    12301 attttggtat ttttttcaat ataatcactc acctttaact tgattatatt agacaaatgt
    12361 ctaatagtca atataatata tcatcatgta taaagaatct ttatattatt ttatgcttga
    12421 gaactaagta gtatagattt atagataaaa aagatattga gagttttatg gaagagggga
    12481 atatattttc tattagagat aagaatatga catatatttt ttattgattt gtacaaaaat
    12541 actatatagt gaataaatta atatcttttc taaattagaa gaggataatc ttattagata
    12601 aagattatcc tctttattga aagtttaagg gatattagag attattaatt tttctctctt
    12661 tttttaacta aaagcaataa tacataaaat tattattgct aatgatactc ttaatgtggt
    12721 atatagtcat agtgtcacat gtttatcaat agttttttca ttaattattg taacgaatag
    12781 taggactcat aaaatactta gaaatatgtt tttagataga ttttaaatat ataatctaac
    12841 ttatatctat aaattaacac tattattctc caattccatt tctagttttt ttataaattt
    12901 attaaattct tctggttgcc tttttccaaa caatactctt acaagagctg aattgatttt
    12961 tccaaatata ttcctcttgc tctcactttc aataaaaata agttttgtat tttctaagtc
    13021 aatatgtact aattcatatc tagtaatagt actttctctt ttattgcttg tttttatctc
    13081 atatactcta tttttttcga aattagttat ttcaacctta aatcttttat tatttttttg
    13141 ttgtacaaat gttcctactg cttttttctc attaaaatta ggaaaattta gtcttgcaga
    13201 ttttgtaaat atattaaata tgtcttttgc agaatatttt aataaaactt cagttttaaa
    13261 tgtattcata tcgttttccc ttctgtatat aaatttaata tttataagtt aatttttaag
    13321 ttaaaatagt ataatatgcc atattaaatc atgttaaaac tatttatata gctaaaacct
    13381 tacctatata agtatgtata ctatcatata aaaataaaat cttaaaatag aaagggttaa
    13441 aaacacagtt tattataatt tttattgttg agtagatacg tactattata tatgattgag
    13501 caagtttttc aatcataaat ttataaagta ttttgcatta aaatttaaac aaagagttaa
    13561 attatttaag attatgaata actaatacat ttagtaaatt actaaatata caaaatgtaa
    13621 atacttatca aaatgtataa atacttatga catctagtta cttacagggt ttaagtagaa
    13681 aaaaatatca aagtaagtat ggcatgatag ttgcttatat atttatcaag aaaggagtta
    13741 ttaagcatgg agttgagaga taaacttgta aacatcataa atacagaaaa taaaaaagat
    13801 ccattaacag atgttcaaat agcaaagttt ttaagtacaa ctagagagaa cataactaat
    13861 ttgagaaaag aacttaatat aagtaattca aggcaaagaa gatatcctta tttaaaatca
    13921 gcaatttcgg caatattaca aaagaataag aatatttcta tatcagaaat tacaagggaa
    13981 ttgatgacag aaggctttaa tatatcgaga agagttgtgg aggaactttt acctaaagaa
    14041 agtttagaaa tggatgtagt agaggaatct gaagaagaat cgaatgaccc atttgaaact
    14101 ctgataggta ataaagggag tcttagaaat gctgtggagc aggctaaatc tgctatattg
    14161 tatccaccaa aaggattacc aacgttgatt attggggaaa gtggagttgg taagagttta
    14221 ttttctagac atatgtatga gtttgcaaag caaaaaaaga tagtaaaaga aagctccaat
    14281 tttgttgtat ttaactgtgc tgattatagt gacaatccac aattattatt atctttgtta
    14341 tttggatata aaaaaggtgc atttacaggt gctgaatatg atacaccagg tcttgtagaa
    14401 gaagctgatg aaggagtttt atttttggat gaaatacata gattgcctcc aaaaggtcaa
    14461 gaaatactat tttctatctt agatagggga aagtttagga gattaggtga aactaatgct
    14521 gaaagaaaag taagtattat gttgattggg gcaactactg aaaatgtaga gactaattta
    14581 ttattaacat tcagaagaag gataccaatg ctaataactt tgccaccatt acatgataga
    14641 ttactaaaag agaaaataga ccttatatac aatatctttc aacaagagtg taatagaata
    14701 aatgcaaaaa tatttgtaga taaaaatgtg attgaaatac ttgtactaaa aaagtttagt
    14761 ggaaatatag gacaattaca aaatatgatt caggttcttt gtgcaagagc ttttatgaag
    14821 tttataaatt ccaaagatga agatagtgaa tcgatagtag tagtagatat aaatgaagtt
    14881 ttaaagctta aagattcttt taaagatgct gcctttcaag agattgaata tacagagata
    14941 agaaaatatt tgaaaaatgc tatatttata ccatttaatt tagaagaatc atcagtaaaa
    15001 ggaagtcttt taagtgatga atatatatta ccagaggaca tatacaaaac aattgaaaag
    15061 aaatattatg acctaaaatc cctagacatt gaagatatag atatagagaa tattttatgg
    15121 acttttattt taaatagatt tgctgactta aagtttaatc tcgatagtaa tgaagaaatt
    15181 ttttcaatga gtgatttaaa tagttttgta agtgatagtt tagttaaact ggtaaaagaa
    15241 cttatgaatg atatagttga aaaaaatatg aatttagaag taaataaaaa tatgttcaaa
    15301 tatctagcaa tacacttaga agaagctata aaaagaatta agctaaatca aaagataata
    15361 aatgtaaatt tagaaaaaat aaagattgac ttttcaaaag aatatgaaat tagtaaaagt
    15421 tttgcaataa aaattgagga atcaattagt attaagattc cagatgatga aattggattt
    15481 ataacactct acataaagtc tgctctaaaa aatgaagtga aaaaagataa agtagggtta
    15541 ataataattt ctcatggacg tatagctaca gagactgtaa atgtagttaa agaactttta
    15601 ggtgtaaagt ttcctgtggc tatagatatg cctttagatg aaaaaccaat aaatatatac
    15661 aataaggcag tagaactgtc aaaaataata gaccaaggga aaggtatatt gtttttggtg
    15721 gatattggtt cacttacaaa cataggtcaa atagtaaata agagaaccaa tataagcact
    15781 aaaactattg atagagtaga cctcctaatg gcactagaag caactagaaa agtttctatg
    15841 ggagaggaag aacttgatga aatatttttt tctttgctaa aagataggat gggatataac
    15901 tatgaattag aaaaacacat agataaatca aatgcaattg taacattatg tcttactgga
    15961 gaaggaactg ctaagtatat aagtaaaacg ttggaagaaa agtatgataa cactaaatgt
    16021 tatcaaatga gtgcattgga tgaaaattta ttttgtaaaa ttgaaaatct aaaagagaca
    16081 aacaatatat tagctattgt tgggacaata aaccctaaga taccaggtat aaattttata
    16141 ccctataata aagaactttt caaaaatttg gatatctatc tatcaaaatc aaaacaacaa
    16201 gatgacagac cagttaaatc gtatgaaaga atgcttgatg aggatttagt gatttttgaa
    16261 ccagatatat attttaaaaa ggatttatta gaatatgtat gttcaattct tataaataaa
    16321 gaatatgtag aaaaagatta cttagattct gttttacata gagaagaaat gcttccaaca
    16381 tattctaaag gagctatagg tgtgcctcat ggagattctt ctactgtaaa tagtacaagg
    16441 tttgtttttg ttaaactaaa gaatcctatt gattggggtg taggtaatgt aaatttcata
    16501 tttatgccag tttttcaggc taatgataaa gagattgtta aaaatgttct tgagatacta
    16561 aaagatgcag aatttatgaa caatgccaat aagtgttttg ataaagatgc ttttagaagg
    16621 attatatttg ataaatttaa acatctgtag ccatagcaaa aaaattgaga ggagttagag
    16681 taatgaatgt agatttaata tcattgggtt tagaatctaa tgattatgaa agtgttattg
    16741 aagaattagg aagtataatg tgtaaaaaag aatatgtaaa agaaacatac ataaatgctg
    16801 tcttggaaag agaaaggaca ttaccaacag gtctagatat tggagaaatg tgtgttgcta
    16861 ttccacatac agattcaaag catgtaaatg agtctaatgt agctgtaggg atattgaaaa
    16921 atcctgtaaa gtttaattca atgatagacc caaaagatag acttgatgta gaacttgtat
    16981 ttttattagc agtaaagaac ccagattcac aagtaaaact gttaaaagat ttaatgtcag
    17041 tttttcaaaa tataaagtta ttaaaaaata taaaaaacgc atcaacaaaa gaagaagtag
    17101 ctaaattatt ggattttata gaaatttaat gtaggagaat tttataaata aaaaattaaa
    17161 atttcaacaa tcaaagtaag tattattttt ttaataggtt taatcttata taaaataaaa
    17221 atttagagtg aaaaattagg aggaaattat tatgaaacaa tttaatgtat tatcagtttg
    17281 tggttcaggg acagttacat catctatggt agcaggtaaa ttaaaagaag tactaggtaa
    17341 taaggggatt tctattacta caacagaagc tagaccaaca gaagcactaa atttagcaca
    17401 atcaggaaga tttgacttta taacatttac gagtccgctt caaacaggcg attatggaat
    17461 accaactata aatgcatttg cttgcctaac tggtataggg gaagaggcat tctttgaaga
    17521 agttttgact gttattgaag gcatagataa gtaagttttg gagacgtgaa tttatagtat
    17581 ttaaatcaat gatttagata ctataataag ctaatattaa ctaaatattt gtggaaaaaa
    17641 cattttatat gttgtcataa attaaaaaat tataaaaata gtcacttaag tattaaatta
    17701 agcttataaa tatttataag taggagggaa aaagatgtta gaatttatga aatctataat
    17761 cgatacattt ggttctgcaa ttatagttcc aattataata ttcataatag caaagatatt
    17821 taaagtaact actaagaaat cgtttctttc tgctgtgtat gcaggagttg ctttacaagg
    17881 ttttacactg attttaaatt catttacacc aataataaca ccagttatca ataggatggt
    17941 tgagagtaca ggagtaaacc taccagtttt tgatgttggt tggcaagcaa catcattggt
    18001 tgcattttca acaagtgcag gtatgatata tttaggtctt ggtatattat tacaaacaat
    18061 tttatttttg ataaaatgga cagacgtgtt tcaaccatca gatttatgga ataactattc
    18121 ttatatggta tggggagcaa tggttatagg agtaacagga aattttcctt taggtatagc
    18181 atgtatggtt ttattaaact tatatagttt attaatatca gagttagttg caaagagatg
    18241 gtctagttac tatagatatc ctaattgtac aataatagct atgcataatg ttgaggcttc
    18301 tgtgtttgca gtattttcag accctatata caataaattg ggattaaata aaataaaatt
    18361 aaatccaaaa gaattagaga agaaattagg gtttttaggt gaaccaatta ctttaggtct
    18421 ttttttaggt atgtttatag gtatattagg taatatgacg agaataaata ctatggaagc
    18481 ttggggagaa ataatgaaag taggtatatc tacaagtgca gtgatggcga ttttccctaa
    18541 agtagcaagt atgtttgctc aagcatttgc acctataaca gaagcagcta gaaagattat
    18601 gcaaaaagct ggaaaccgtg agtggtacat agcagtaaat gatgctgttg gatatggtga
    18661 acctgcaact ttaatatcag gtcttatatt aattccaata atgcttgtaa tatctatggt
    18721 acttcctggt aataaggtat taccagttgt ggatttgttg gcaattccat atatggttca
    18781 aggtttagtt gccatacata atggtaatat accaaaagta ttagtttcag gtataatttg
    18841 gtttggatta ggattatatg tttgtacatc tacagcacca ttatttacag atatggctac
    18901 aaatataggt gtagctatac ctgcaggagc aatgttaata acaagcttta atatactagg
    18961 aaaacctttg atgggattag ttttctttgc atttctaagt gcaaatccaa tttatatagg
    19021 tctttcagtt gtaatttact ttgttctatg ggcattgttt agaaagaaca aaacaagtat
    19081 tcttgattat ttagaaaaac aagcacttaa aaatgtagaa gaagagcctg tagcagttta
    19141 agtttataaa aatgaaataa gaataattaa aggagtttat aattgatttt atataagtat
    19201 cagataatac ctgagaaata tttaaatgaa atatagactt ctttttattt gataatattt
    19261 gtaattataa tgcattgtat ttttatagtt gagttttctg tataacctgc tatttaaaac
    19321 atactctttt atatttatac agtaatgtgg tttaatttta taaaatgtaa tctctactgg
    19381 taaaagactt tgtatataag gcatttgcct gaataaacca tttatctttt gtatggcata
    19441 gatattgcta tatagaagta tatagcatga aaggagatat ttatatggga gataaaatga
    19501 gagcatcagt attgtataat gtaggtgatg tcaggtatga aatggtagat ataccagaaa
    19561 taacagatac gcaagtgctt gtaaatgtaa aatatgttgg gatatgtggt tcagatttgc
    19621 ctaggtctat ggtaagtggt ttgagtggaa atacaaaata tccattgatt ttaggacatg
    19681 aattttctgg tgaagttgtt aaaattggag aaaaagtaaa gcatattaat gtaggagata
    19741 gagtagctgt tgcaccatta gtaccttgtg gaaaatgtga ttattgtaat gaaggtaatt
    19801 ttggcttgtg tgatgattac aatataattg gaacaagagt aaatggagcg tttgcagaat
    19861 atgtaagagt tccagaagaa catattttaa aactaccaga tacattggat tatgaaactg
    19921 ctgccggaat tgaaccagca actatagctt atcatgggat ttcaaagtct aatattagag
    19981 taggagatag tgtagttgtg ttaggatgtg gtccaatagg tcagtttgtt atacaatggg
    20041 caaaggtgtt tggtgcatct aagattatag cagttgatat ttttgatgaa aaattagaac
    20101 tttctaaact tctaggagca aactatatac ttaactcaaa agaagtaaat gtaatcaaag
    20161 aaataaaaaa aataacaaat ggtggtgctg atgttgtaat agagactgct ggtagccgat
    20221 ttacacaaga acaatctcta tttgtagcaa aaaagagagg aaatatagtg tttgtaggta
    20281 tatcacacac agaattacca cttagtgcag acgctactga gtgtatatta cgtggagaac
    20341 ttaccttgaa aggttcttgg aattcatatg caagccctta tcctggaaga gcatggactg
    20401 caacattgga ttttatggga aaaggtgaca ttatttttaa acccatgatt tcacataaaa
    20461 taggtcttaa tgaagtgggt gactttttaa gtaaaatgag caagagagaa attaatttca
    20521 ataaaatcct agtagaaata taaaaattta ataaaaaatt ctaaaaacaa ttaaatttta
    20581 taactatgac attaaataaa atataggagg aagaaaagtg ctagaaattt taaaagaaga
    20641 agtattaaaa gcaaacttag aattacctaa gaaaaagcta attacttata cttggggaaa
    20701 tgtaagtggg atagatagag aaaaaggatt agtggtgata aaaccaagtg gtgtagagta
    20761 tagtaatatg actgtagaag atatggttgt agttgattta gagggaaatg tagttgatgg
    20821 caaactaaga ccctcctcag atactccgac tcatctagtt ttatataaag agtttgaaga
    20881 attgggggga atagtacata cacattcaag ttgggcaaca atttgggctc aaataggaaa
    20941 atctatacca tcttgtggta ctactcatgc agattatttt tatggaagca taccatgtac
    21001 cagaaaaatg actagagaag aaatttgcaa tgaatacgaa aaggaaactg gaaatgtaat
    21061 cattgaagaa tttatgggaa gaaatccaat tcattgtcca ggaataattg taaatgacca
    21121 cggacctttt acatggggaa aagatgcaaa tgaggctgta cacaatgcag tagttttaga
    21181 agaagtcgca aagatggctt attatacaga gttaatgagt cctgataata ttatggataa
    21241 ggttcttatg aataagcatt tttcaagaaa acatggaaaa aatgcatact atggtcaaaa
    21301 atgattatct ggaggtgaat tatgctagac tctttaaaag gtaaagttgt ggaaatagca
    21361 attaaagcag agcattatgg tttatgtaaa gaaaagactg gtaattttag tattagagat
    21421 aaggatactg gatatatact tataacccca tcaggtatag gaagagaaga attaaacgtt
    21481 gaacatattt gtgtattgga tttgaatgga aatgtaatag aagttgagaa aggtattaag
    21541 ccaagtagtg aggtattgat gcatattgaa atatataaaa taagggaaaa tataaatgct
    21601 atatcacata ctcattcttt atatgcaaca gcattttcag tagcaaagaa accaattata
    21661 ccaattgtat atgaatcagt taattatggc ggttatgtag atattgctcc atacgaaaga
    21721 ccaggaactt taaaattagc taagagcgtt tcaaaattac tgatagatac agatgcatgc
    21781 ttattagaaa gacatgggtt gacaactgta ggtaatgatt tagacgaagc attattaaaa
    21841 tcaagatatg ttgaagaggt agcagaaata tactataggt ctttagtatt aaatcaattt
    21901 aaagaacttg aagtagtaga tgttgatgag tttgaatctt ggaaataccc agaagaaata
    21961 aagttttaat ctaaataatt ttcattgaag tggttaatta aaatatatgt attatttata
    22021 tcaattacta tgttttatta tattaatcac cgtattttgt aaatatattt attacatttt
    22081 ttcaaaaaaa tatagaatcc gaaagccata taaaattgac ttgggattct atacttttta
    22141 tttatgcatt tttctttatc tgattttctg gagaatttat atgttcaaaa tattttttag
    22201 tttcagatac tattataggt gataatgcaa gtaaagctat aaggttaggt attgccataa
    22261 gcccatttac aatatcagca attatccaaa caacttctaa ctttaagaaa gcacctaata
    22321 acaccatagc cacaaataca gttctatacc cattcatgcc ttttacacca aacagaaatt
    22381 caaaacatct ttctccataa taactccatc ccaaaattgt agtaaatgca aataatgtta
    22441 aactaactgt aagtaatatt ggtccaaatg taggaattgc tccgttaaaa gctgcttgtg
    22501 tcataacagc tccatttagg tctgaacacc acactcctga aataacaaga gagaatccag
    22561 taagagtaca gattataata gtatctataa aagtacctgt catagatata agaccttgtt
    22621 ccgctggcca ttcagtttta gcagcagctg cagctattgg agcactacct agaccagact
    22681 catttgaaaa tacaccacga gcaataccat ttctaattgc cagtgacata gttgctccta
    22741 aaaaaccacc agtagcagca gtaggtgtaa atgcactttt aattattaac ataaaagttt
    22801 cagggatttt attccaaaaa gatattaaaa tgattccaca gattacaaca tatataactg
    22861 ccataaaagg tactatttta gtagctacat tagatatact tctaagtcct ccaaatacaa
    22921 ccaaagctac aaatactgct aatacaaccc ctgtaattct agggtctatc ccgaaactat
    22981 tatttattgc atctgttatt gaatttactt gagtaaaagt accgattcct aaaagtgcaa
    23041 ctagtatacc acttatagcg aaaaatattg caagaggtct ccatttttct cccataccat
    23101 ttactatgta atacattggc ccaccagaca cttgaccatt tttatcttta gttctgtatt
    23161 ttattgcaag aacaccttct gaatacttag tagccattcc aaagaatgct gctatccaca
    23221 tccaaaaaag tgcaccaggt ccaccagctt tgatagctgt agcaacacca acaatattac
    23281 cagtaccaac tgtagcagct agagcagtac ataaggctgc aaaacttgaa acatcaccac
    23341 tacctttgtt ttcagcagag aatattaact taagagctct tggcagttta gttatttgaa
    23401 caactccaag cttaacagta agtagtatac cagtaccaac taatagtacc agtaggggtg
    23461 gtccccaaat aaaagcatca actttgttta aaatatcaat tagattcata aaaacactcc
    23521 ctcatttttt ttgctttaca taatcaatag aatttttaat gaagtaattg acaggaaata
    23581 agtataattt attaataatt tctagtgtaa taaaaaaagc taaatctaaa aaagattcag
    23641 ctctggatgt acactaatta gcaaccaaaa taaaagtatt agtaaatact gtttttggta
    23701 gataatccct gtccttttgc ctgagagatt gagcataaaa gccttgcccc ttcggcgccc
    23761 ataatgggtc tctccagagt tccgtaaatt ttacagtcct acttttaaaa gtacctgaga
    23821 gtgttactcc ttcggtgtgc tatatagcaa ctcttctata aaatcttaac cggatattga
    23881 tttgtcatag cttattgtat taataatatt aatataagtt tttatgtttt gtcaactaaa
    23941 aaattaaaaa acaaagaaaa aatataaaaa tgtaagccta tttacgaatt ataaacttag
    24001 aaagatgagt aatatacgta aaaatgaatt ttgtaataca catacaaatg aagtgtaaaa
    24061 taagttttaa ataaaaaaat aataaaatct taaataatta attagatatg attaaattat
    24121 ggtaatatta tagttacgat attaagaaaa gttctattta gttagatttt aagcgtaata
    24181 tgagattatg tatagagaaa ggatgtattt ttagatggga aaaatattag tgctagcaga
    24241 aaaaccttct gttggaagag atatagcaaa agttttaggg tcaaaaaatg aaaaaaatgg
    24301 ttatatagag gggccaaagt atgtgataac ttgggcacta ggtcatttgg ttacacttgc
    24361 tgaccccgaa agttatggag aaagatataa atcatggagc ttagaagacc tgccaatact
    24421 tcccaagcac cttaagactg ttgtaataaa aaagagtgga aagcagttta atacagttaa
    24481 atcgcaaatg aatagaaatg atatagatga aatagttata gcaacagatg caggtcgtga
    24541 aggagaactt gtagcaaggt ggataataga aaaatcacag gtaaaaaaac ctataaaacg
    24601 tctttggatt tcttcaagta cagacaaagc aataaaagac ggatttgcaa aattaagaag
    24661 tggaaaagaa tatgaaaatt tgtattactc agcaatagca cgagcagaag ctgattggat
    24721 tattggaata aatgcaacca gagctttgac aacaaagtac aatgcacagc tttcttgtgg
    24781 aagagtacaa actcctactc ttgcaatgtt gcttaaaaga gaagaagaaa taagaaattt
    24841 caaacctaaa gagtattatg gattagaatt aattgctaca aagggaaatt cagatataaa
    24901 atttatttgg aatgataaaa acaataattc ttcaactttt agtaaagaaa agatagaatc
    24961 catattaaaa aaagtaaaag gtgtagacat aaaaatatct gaagtaaaca agtcatataa
    25021 gaaaaaatat tcacctgcac tctatgattt aacagaatta caaagagatg caaataaaat
    25081 gtttggattt tcagcaaaag aaactttatc tataatgcaa aaactgtatg aacatcataa
    25141 agtacttact tatcctagaa ctgattctag atatttaaca tcagatattg ttggaacatt
    25201 aaaagataga ataaaagctg taaatattag tgattattca aagatttgta ataaattatt
    25261 aaaaagcaat attatagcta ataaatcgtt tgttgacaat tcaaaggtaa cagaccacca
    25321 tgcaataata ccaacagaac agaaggtatt catgagtgat tttagtgaca aagagcgaaa
    25381 aatatataat cttgtagtaa aaagattttt agcagtacta tctcctcctt ttgaatatga
    25441 gcagacaact ataagtgcaa ctgtagaagg agaaatattt aaggctaagg gaaacaaagt
    25501 aataaattta ggttggaaag aaagttataa agatattgaa gatgaagatg agtatgaaag
    25561 tataccaaat gttttagaaa aagatgtact taaggtcaag ggattaaaga taacttcagg
    25621 aaaaacaaat ccaccaccat acttaaatga gggaacatta ttgactgcta tggaaaagaa
    25681 tggtctaggg acagttgcta caagagcaga tataatagaa aaattattca attcattttt
    25741 aatggaaaag aaaaataaag agatatatat aacatcaaaa ggaagacaat tattagattt
    25801 agttccagta gacttaaaga caccagaact tacagcttca tgggaaaaga aattattgga
    25861 tatttcaaag ggaaaactta gtaaaaatac attcataaat gacatgaaag attattcaaa
    25921 agatattgta ctagaagtaa aaaatagtga taataaattt aaacatgata acttaactaa
    25981 aaatagatgt ccagaatgtg gtaaatttat gttagaggta aatggtaaaa aaggtaagat
    26041 gttgatttgt gaagatagag agtgtaatac tagaaaaact atatcacaag taaccaattc
    26101 aagatgtcct aactgtcata aaaaattaga acttcgtgga gaaggtgagg ggagaatctt
    26161 cacttgtagt tgcggataca gagaaaagtt gtcttctttc aacaagagaa agcaagaaga
    26221 aaaacagaag gcatcaaaaa aagatgttaa tcaatattta aaaaatcaaa ataaaaatca
    26281 agagacattt aataatccat ttgcagaagc attagctaag ctaaaaaaca aataatttta
    26341 aagttattat tatacacaat tataacttta actgtatatt agttatatgt actgatataa
    26401 tatagtgtat agtttaggca tgaaagtagg gctgttaaat ggatttaagt ggcatattta
    26461 aatactattg taaagagtgt gaaaatacat ggaataattc gagtgttgaa ttatttgagg
    26521 atatagaaac gtatagtaaa gattcacaaa aaaagaggga aaaagaatta gataaattta
    26581 taaatacaat atcagttcat ttagagaggt atccaagtga tgctgtattg agaaaaatgt
    26641 gggtaaaaaa gggcgaggtt ttcttacaaa agacattgga aaaagaaaat atttttaagt
    26701 tagaaaaaat ggatgtagag gatagaaaaa aatttttaga tataacaaaa cagtttatta
    26761 gagatgctag aaaatttgat gatgatttac ctataggtga tattatgcaa gctatgagga
    26821 atgtatggat ttcaaatgca ttacaattat tatttggtaa agaaatatat tattcaaaag
    26881 ctaactttgc atatagtatg ttatatccat atactgataa ttatttagac aatacaaata
    26941 tagataaaaa tgataagatt ttatttaata actggttaga aaaaaggctc ctgggagaac
    27001 atattaaatc taaggattat catgaaagta aagtatctca aatgatagat tatattgaaa
    27061 gtgtataccc tagagaaaag tttacacaag tttatgaatc gttattatta atatttaaaa
    27121 gtcaagtaaa tagtttaaaa caacatggta aggaaaatca tttgtgtaaa gaagatttat
    27181 tatccatttc tattgaaaaa ggaggttcat ccgttttagt agatggatat ttaataagtg
    27241 gatttatgac aaaggaagaa atagagtttt gtataggata tggattttta ttacaaatat
    27301 ctgatgattt acaagatata aaagaagatt taaaatacaa ccataaaact attattacag
    27361 agatgtcaaa agagggtact ttagataaag ttgtaaataa actaataaat tttactattg
    27421 agttaataga tagttttaaa attaataata aaaataaatc tgtaataact atgataaaga
    27481 atgattgctt aatgttaatt ttattttctg tagtttataa tgctgaattt ttttctgtag
    27541 gatatataaa agaagtagag aaatttattc catatacaat agattattca ttagagattg
    27601 aagaaaaaat aaaagaaaaa tttaaaaata tagatgtttt aaataatgaa aatgaatata
    27661 aagaaatgat tgatattatt tgtgcagagt agaaaaaaag ggagtgtctt agaatagact
    27721 ttttagttta tgaggcactc tttttgtata aaatcaaatg tattttcatc attttaacaa
    27781 ttaaaaagag cttatccctg tttgagaaag ccctttttaa tttaaataaa aatgtcaagg
    27841 ttaaactatt taaatgcagt aataatccca ttatcatgta agttttttat gattgtagct
    27901 gcgataggga atccaaataa tccagctatt ccaaatagtt gagctccaat aaacatacac
    27961 attaatgtta ttaaaggata taggccaatt tggtgtccta ctacctttgg ttctaatatt
    28021 tgtctaacaa cggttataat tatgtatagt attaaaagtc ctattgctaa attaatattt
    28081 ccattaatga gtgagataat catccaaggt gtcaaaatac ttcctgtacc caagactggc
    28141 aaaatatcaa caagtgctat gatgacagat atagtaaatg cattttcaac ctttaggata
    28201 gtaagtccaa taagtagttc taagaatgtc actgatagta gtattgcata ggcttttagt
    28261 agttttaaaa gagcattaat actgctattt tttatattta ttatcataaa ttgacttttt
    28321 tctggaaatt gttttaagat aaagtttact attttttgat agtcaaaagt aaagaaaaaa
    28381 gaagcaatta aagtaataat aagttttaac ataaatgcag gtgttttact tgcaatacta
    28441 gcaatagcat tgagtgcatt tgttgaaata gaagctacta aagacatcat agattgactt
    28501 atattatccc aaccaagtat taatgatagg ttaacattag gtgtactagc atctatcttt
    28561 gaaaataaag tatttagcgt tggttgtata cttgatttat atatttcagg aagtctaaaa
    28621 aatacatcac ttacacttgc aaatatcttc acaccaaagc taactaataa aaatatacac
    28681 aacccataaa aaattaaaag tattactata gaaataagtg tacgatttaa atttgtatta
    28741 tttgtaataa gtcttataat aggtcttaag atggatgcaa tgataaaact gattacaaaa
    28801 ggcattaata atggcaataa gaattttaat acaatatata caatacttgt tattaaaact
    28861 acatatgtaa atttaattat aaaattcttt tgcttttgaa tatccataat ataaaacctc
    28921 caatgtattt gatattattc taaatatggt cttttttaca gaaaatatac tactgtatat
    28981 tgattataaa ctataaagct tatgtaaagt aaataaatta tgtaaaattt aggttaattt
    29041 tgtataaaga gatactttta tattttacaa ttaataacca aatactaaaa aatattttaa
    29101 ataaagaact taaaattgtg ataaaattaa tttatagata ttaaaaaaag tataatatat
    29161 ggtttttaag aggtgctaag atgaaaaatt ttagagattt aggtgggaac aaaactgagg
    29221 atggcagaac tgtaaagaag ggattatttt ataggtctgc aaaattatca aatctaagtg
    29281 aaaatgatat aaagatttta aaagagttaa atattaaata tatatttgat tatagaagtg
    29341 atgaagaagc tcgtaaacat ccatcaacaa taatatcaaa tataaaaaat ataagaatac
    29401 cagcaatgag agagctagaa gaatcaggag gaagttttgg ttctattgaa gatatgattg
    29461 atggattgtt tgaaaaagat ggtgcattta atatgttaaa taatagttac tataatttgc
    29521 ctataaataa tccgtcatat aaaaaattag tagaacttat aagagactat tctaatttac
    29581 caatattaaa tcattgtaca gctggtaagg atagaactgg tgtggggagt gctataattc
    29641 ttatgatttt aggggtatcg agagagaata taatgaaaga ttatctaaaa agtaatgatt
    29701 ttgcagataa agagatagaa agatttatag aatataagcc taaatttaag gacattccaa
    29761 aagaaaattt aaaatatata tttggtgtaa atgaagagta tatgaaaact gcatttagaa
    29821 gaattgatga agaatacatt agtgtagaag catatttata tggagaattt aatttaaata
    29881 aagaagaaat aagaaaatta agaaatcaat atctagagta aaaggtgagt ttatgagttt
    29941 aaatataaca gcaagacaga taaatataat aaaaatattg ttaaattcaa aagatacaat
    30001 tagtggtatt gccttaagtc aagagatagg atgttcttca aaaactatac aaaatgaaat
    30061 aaaggatatt aataaacaac ttagaaatgg taaaattttg tcaataagag gagttggtta
    30121 taaactagaa ggaagttttg atgaggtaaa tctaaatagc aatctctatg atgatgtaga
    30181 tagagtagaa tatataatca gaaaaatatt aaccttaagt aatgaagaaa aaaatactat
    30241 aaaacttgaa gatttagccg attctatgta tgtaagtgtt tcaacagtaa aaaatgattt
    30301 aaaagaagta aaaagaatat tggaaaagta ttcaatatca gttaaatcta agcataaaca
    30361 aggtatatgt gttttggaaa gtgaagataa gatattaaat tgtatagttg acttaagcaa
    30421 taagagagat aatcaattga gtttaaatga ttttttaaat gacgatataa aggaaagaaa
    30481 actattaata aaaaaattat tattagacat attaaatgaa aatggattag tattaactga
    30541 tatagaattt aagagtgtgt taaataatat attagttatg ttaagcagac ataagtataa
    30601 tgaaaaagaa tttatagaga attatataga aaattatagg agcaaaagaa gctcaatttt
    30661 agaaaatgaa caaaacaaag aaattataat aaattcaata aaaacatttt gtaaaaattt
    30721 aaaattagct acatcaatag atataagtaa tgataaggta tttgaagagt ttttatataa
    30781 gcatattagt agtttatgta aaaaaataaa gttaggtata aatcaatcac ctataatggc
    30841 tcaagatata aaaataaaat atccatttgc ttttgaacta gctaatatag ctaaaaagac
    30901 aatagaaaaa gatttgaaga tagaaattaa tgaagatgaa gttgcaaaca tagctctaca
    30961 tataggagga gcagttgaaa gagcatctta taataataaa gataaggttt tcaaaactat
    31021 aatagtttgt acttcaggca taggcacatc aatgttaata aaagctaaat tagagaatat
    31081 atttaaggaa aaattagaaa tactaaagat tattccttca tacttagttg aatatataaa
    31141 agttatagat gttgattttg taatatctac tgttcctatt gaagtaggag atgtgccagt
    31201 aataaatata tctccaattt taagtgagaa agaaattaga ttaattgaaa aatatataga
    31261 aacaggaaat atatacctag atttagatat aaaaagcctg tttgatagtg aattattttt
    31321 tactgatttg gtgtttaaaa ggaaagaaga agttatagat tttatggcaa gtaaacttgt
    31381 acaaaagggc tatatagatg aggatatgaa aaaatcttac tttgagagag aaaaaatagc
    31441 aacaacagaa atagggaata tggtaactat accacatgga gctattggaa aaatttttga
    31501 taataaaata gcaattggta tattaaagaa gcctatttct tgggaaatat cagatgttcg
    31561 tttaataata atgttagccc ttgataaaga taaaatatta gattatgaag taatattttc
    31621 aaaaatatat aaaagagtag attctatagc aaaagttata agtatatgtg aaaatagtag
    31681 ttttgaaaaa tttgtaaatt tatttaatta gatgggtagt attgtatata cacctgtaaa
    31741 tttggataat ataaaactaa ataagataaa aacgataaat ttctctttta attaagagaa
    31801 attttttgac tttcaaggat attaatataa agttaagata taaacataaa gcaaataaat
    31861 aaaaaagagg tggcaatatg atttatacag tgacattaaa tccatcaata gattatatag
    31921 ttaagctaga tgagctaaag acaggaagta caaatcgtgt aaaagaagaa tacgtttatc
    31981 caggaggaaa aggtataaat gtatctcgca tacttaagga acttggaaat gataatacat
    32041 ctttaggttt tataagtggt tttactggag aatatataat aagaacattg gaagaaaaag
    32101 aattgaagac agattttata aagataaaaa gtggtttttc aagaataaat gtaaaaataa
    32161 aggcgttaaa ggaaactgag attaatggac aaggtccaaa tatagatgat gaagacattg
    32221 atactttata taaaaaactt gataagctaa atcaagatga tattttaata ttggcaggaa
    32281 gtataccatc tactttagat gaaaaactat acgaaaatat aatggctaga ttagaaaaaa
    32341 agaatataaa agttgtagta gatgcaacta aaaatcttct tcttaatgtt ttaaaataca
    32401 aaccttttct aattaagcct aataacgacg aattagaaga actatttggt gttaaattaa
    32461 attcaattga agacatggta aaatatgcta aaagattaaa ggaaatggga gctataaatg
    32521 tcttggtatc aatgggaaaa gatggagcat tgttgattac agaggaagat gaagtgctta
    32581 taagtgatgt ccctaaagga aaagtaaaaa attctgtagg agcaggtgat tcaatggtag
    32641 caggatttat atctgggtat ttgaatactg ggaaatatga ttatgctctt aaattgggag
    32701 cagcttcagg tagtgcaact gctttttcat atgacttagc caaaagagaa tacattgata
    32761 aattggtaaa tgagatatct gtaaagcagt tttaaaatat taaaaaatat atagaaaggt
    32821 aattaatatt tatgaaaatt atagatttat tagatgaaaa gtcaattaag ttaaatttaa
    32881 attcaaaaac caaatcagaa gcaatagaag aattagtaga tttggtcgca aatagtggaa
    32941 atttaaatga taaagaaaac tacaaaaaag caattttggc tcgtgaagaa atgagtacaa
    33001 caggaattgg agaaggtgta gctatacctc atgctaaaaa ttcttcagtt actaaagctt
    33061 gtatagcagc agcagtgtca aaagaaggta tagattatga atcgtttgac ggaagtttat
    33121 caaatctatt ttttatgata gctgcaccag acggagctaa caatactcat cttgaagtat
    33181 tatcaagatt atctacaata cttatggatg aagactttag aaataaactt ataaattcaa
    33241 gctctgaaaa agaatttaag gaaataatag ataaaaagga aagagaaaaa ttcagtgaag
    33301 aatatcaaga tgagaaagtg gctgaaaaaa atataataga tactaaagag aatgatgcta
    33361 ataagtatcc taaggtttta gcagtaacag catgtccaac tggaatagca catacattta
    33421 tggcagctga aagtttaaat aaaatggcag aaaacaaagg tgtatcaata aaagtagaaa
    33481 ctaatggttc agcaggtgtt aaaaataagc ttacaaaaga tgaaatagaa aatgcaacat
    33541 gtataatagt ggcagcagat aaaaatgttg agatggctag atttaatggc aaaaaagtca
    33601 taaaaactaa ggtggctgat ggtatacaca aagcggaaga acttatagac aaggctgtaa
    33661 atggagatgc tccaatttac catggaggag atggaagtca caacgaatca aatgaagaaa
    33721 gtgaaagtgg atttagaaaa gtatacaaac acttaatgaa tggtgtatct aatatgttac
    33781 catttgttat tggtggaggg atacttatag cgatagcctt tttacttgat gattatacta
    33841 ttaacccttc aaactttggt tcaaatacac cgatagcagc gttttttaaa gggataggag
    33901 ataaagcttt tggatttatg ttacctgtac ttgctggata tatagcatac tctatatctg
    33961 atagaccagc gtttgttgta ggatttgtag gaggtgctct agcaggtgat ggtggttcag
    34021 gatttttagg tgcactactt gcaggtttta tagcaggtta tttagtagaa gggcttaaga
    34081 aaatatttag tgtactacca gcttcattgg aaggaataaa accagtattg ttataccctt
    34141 tacttggaac acttttgatg ggaattataa tgacattttt agtaatacct ccagttacag
    34201 caattaataa tgcaatggtt ggatttttaa atggtttagg cggaacttca aaaatagttt
    34261 taggattagt attaggtgga atgatggctg tggatatggg tggacctgta aataaagcag
    34321 catatgtatt tggagttgca tcacttgaat cagggcagtt tgaaataatg gcagcagtaa
    34381 tggctggtgg tatggttccg ccacttgcta tagctcttgc aactacattt tttaagaata
    34441 gatttactaa agaggaaaga gattctggta aagttaatta tgtaatggga ttatcatttg
    34501 ttactgaagg agctatacct tttgcagctg gagacccact tcatgttata ccagcttgtg
    34561 ttggaggttc agcagtggca ggagcactta gtatgttgtt caacgcagct cttcgtgcac
    34621 ctcatggagg agtatttgtt ataccagtgg ttacccatcc atttgcatat atattagcta
    34681 tagctgtagg tgctttggtt ggaatgatgc ttttagcatt acttaaaaaa ccattaaatc
    34741 aagaagtata gcaattaaag gatattcaag aacttaaaat atgatgtttt ataaaaatta
    34801 atatattgct gaattatttt aaaataagga ttttattaaa ataaggattt tattaaaata
    34861 aggattttat gaggctgtta tataacgatt tgtaattgtt a
//
LOCUS       DAELXX010000030        33357 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.030, whole genome shotgun sequence.
ACCESSION   DAELXX010000030 DAELXX010000000
VERSION     DAELXX010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33357
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.030"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            169..309
                     /locus_tag="KPH29_001412"
     CDS             169..309
                     /locus_tag="KPH29_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467031.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(484..804)
                     /locus_tag="KPH29_001413"
     CDS             complement(484..804)
                     /locus_tag="KPH29_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBG1467032.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(924..1661)
                     /locus_tag="KPH29_001414"
     CDS             complement(924..1661)
                     /locus_tag="KPH29_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBG1467033.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            1876..3699
                     /gene="acd"
                     /locus_tag="KPH29_001415"
     CDS             1876..3699
                     /gene="acd"
                     /locus_tag="KPH29_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HBG1467034.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISESNGWSKIKYNGKDAYIS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            3914..8212
                     /locus_tag="KPH29_001416"
     CDS             3914..8212
                     /locus_tag="KPH29_001416"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBG1467035.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEILPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            8416..8877
                     /locus_tag="KPH29_001417"
     CDS             8416..8877
                     /locus_tag="KPH29_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBG1467036.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            8907..10061
                     /gene="nusA"
                     /locus_tag="KPH29_001418"
     CDS             8907..10061
                     /gene="nusA"
                     /locus_tag="KPH29_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBG1467037.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVKSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELGTSEDNFSEEE"
     gene            10068..10349
                     /locus_tag="KPH29_001419"
     CDS             10068..10349
                     /locus_tag="KPH29_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBG1467038.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            10339..10650
                     /locus_tag="KPH29_001420"
     CDS             10339..10650
                     /locus_tag="KPH29_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HBG1467039.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            10672..12612
                     /gene="infB"
                     /locus_tag="KPH29_001421"
     CDS             10672..12612
                     /gene="infB"
                     /locus_tag="KPH29_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBG1467040.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            12655..13035
                     /gene="rbfA"
                     /locus_tag="KPH29_001422"
     CDS             12655..13035
                     /gene="rbfA"
                     /locus_tag="KPH29_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBG1467041.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDNLIKRVNEKNAQQNEDENYDE"
     gene            13016..13999
                     /locus_tag="KPH29_001423"
     CDS             13016..13999
                     /locus_tag="KPH29_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBG1467042.1"
                     /translation="MKIMMNNMDSIINYIYEGDYFIVTSHISPDGDNVGSTLSMYYTL
                     KNLGKNVYYVLDDEAPLNLRFLVEGVKIYKSNEFNMKNYSLIALDCGDKMRICVSDEI
                     KNNASKLICIDHHASNDSYGDLNYVDIDASSTCELVYNLLVRFQQTKDINIINEDIAT
                     CLYTGLVTDTGNFSYSNVHASSFEMAKNLLVLGAQKNTIIQNIYQSNSSDYYKLLGEA
                     LKGLEIFDSKVSSIVLTQDMMNRNNISFNDVDGITTYTRDIKGIEVGILFKEKKQNEI
                     KVSFRSKNYVDVSEIAKLFGGGGHVRAAGCTIRDSIDNAKKMVLEAVLKSI"
     gene            14012..14917
                     /gene="truB"
                     /locus_tag="KPH29_001424"
     CDS             14012..14917
                     /gene="truB"
                     /locus_tag="KPH29_001424"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBG1467043.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEVVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            15048..15977
                     /locus_tag="KPH29_001425"
     CDS             15048..15977
                     /locus_tag="KPH29_001425"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBG1467044.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEYAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            16081..16338
                     /gene="rpsO"
                     /locus_tag="KPH29_001426"
     CDS             16081..16338
                     /gene="rpsO"
                     /locus_tag="KPH29_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBG1467045.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            16578..17423
                     /locus_tag="KPH29_001427"
     CDS             16578..17423
                     /locus_tag="KPH29_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBG1467046.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            17548..19659
                     /gene="pnp"
                     /locus_tag="KPH29_001428"
     CDS             17548..19659
                     /gene="pnp"
                     /locus_tag="KPH29_001428"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBG1467047.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            19925..20668
                     /locus_tag="KPH29_001429"
     CDS             19925..20668
                     /locus_tag="KPH29_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG1467048.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKPGKLSDVFK"
     gene            20826..22073
                     /locus_tag="KPH29_001430"
     CDS             20826..22073
                     /locus_tag="KPH29_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBG1467049.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGT
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAICLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINNVNINSIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            22108..22353
                     /locus_tag="KPH29_001431"
     CDS             22108..22353
                     /locus_tag="KPH29_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBG1467050.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGGILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            22363..23586
                     /gene="dapG"
                     /locus_tag="KPH29_001432"
     CDS             22363..23586
                     /gene="dapG"
                     /locus_tag="KPH29_001432"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBG1467051.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEENIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            23699..24433
                     /locus_tag="KPH29_001433"
     CDS             23699..24433
                     /locus_tag="KPH29_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HBG1467052.1"
                     /translation="MKDYREDNERFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            24556..26967
                     /locus_tag="KPH29_001434"
     CDS             24556..26967
                     /locus_tag="KPH29_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBG1467053.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDEEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKSNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKSSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            26970..28028
                     /gene="mnmH"
                     /locus_tag="KPH29_001435"
     CDS             26970..28028
                     /gene="mnmH"
                     /locus_tag="KPH29_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBG1467054.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNEVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESVFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            28022..29356
                     /gene="rimO"
                     /locus_tag="KPH29_001436"
     CDS             28022..29356
                     /gene="rimO"
                     /locus_tag="KPH29_001436"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBG1467055.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFSELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHQIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            29343..29885
                     /gene="pgsA"
                     /locus_tag="KPH29_001437"
     CDS             29343..29885
                     /gene="pgsA"
                     /locus_tag="KPH29_001437"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBG1467056.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNVPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
     gene            30173..31219
                     /gene="recA"
                     /locus_tag="KPH29_001438"
     CDS             30173..31219
                     /gene="recA"
                     /locus_tag="KPH29_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBG1467057.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGTSVSKEIVEE"
     gene            31395..32936
                     /gene="rny"
                     /locus_tag="KPH29_001439"
     CDS             31395..32936
                     /gene="rny"
                     /locus_tag="KPH29_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBG1467058.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
BASE COUNT        13514 a         3533 c         6361 g         9949 t
ORIGIN      
        1 tatatgaatt ttaataggta aaatatccta ttgttttgta gatatatcct aattatgtca
       61 aaaaataaaa aaatttttat ttattagagt ttttggaaat aataataaca gatattataa
      121 ttaattaaca gtttttgtaa gtatttgaca atagagaggg tgtaacaaat gggaataata
      181 gagtcagcat caaaattggc tgagatggtt catttattgg ctgttgaaaa ggggataact
      241 gatatagaag catgggatga agctgtgaaa gaatacagca aaatttatga agaaagaaga
      301 aatgaataga atgataaaaa atgaaaactt gatgataaaa aataacttta tttaaagaaa
      361 ttcaattttt atagaaatat taaaaataat ataaataaaa agatggtatt tcaaatgtaa
      421 gtaaaaaatc attttgggat accatcctta ttatttattt taataatata taagtaataa
      481 atcttactta tccatatttt ttatgtaacc tcttttttct agctttttta tatgctcctt
      541 acgtatttca tcaagattaa taccatattg ttcttctagc ttaaacatca tagtaacaca
      601 tgtctgagct acatccatta gttccttagc agttttcttt ataatatcaa cttcatctaa
      661 atcattattt tctccactta gatttctgaa tttacctata cattcagcta attcaccttg
      721 ttcttcaccc attttgataa atgtagattc tagtgttggg tctaaattat taagctctgg
      781 taaagatata gtttttagtt tcataaaagc tcctttcaag ttattcgtat ttatactaaa
      841 acatcataac aaaaataata ctaaaaaaaa actttaaatt caacataaaa acttacatat
      901 ataataaaaa tcgtgaaata aatctatatt tgtcttaata cagcctcata taaaacttct
      961 ccaataggtt tatttattac aagagaagct gacttatcat agactgtatt tgctttatta
     1021 ataagagcaa tataatcacc tttgtaataa tctataaaac ttgctgctgg gtacacagct
     1081 aatgatgtac ctcctataat caataaatca gcattactta tagcagaaat tgttttagta
     1141 atgacatcac tgtcaagagc ttcctcataa agtactacat ctggttttac tatagaacca
     1201 cagttgtcac aataaggaat attacctccc agatttaaca tagattctaa gtcaaaaaac
     1261 ttaccacatt tagtacaata atttctatgt acagaaccat gaagttctag cacattttta
     1321 cttccagcca tttgatgaag tccatcaata ttttgagtta tgacagcttt aagcttaccc
     1381 atttcctcga gttttgctaa agctatatga gcattgttgg gttttgcatt aggatatatt
     1441 aatttatcct tataaaattc aaaaaattct tctggatatc taacaaaaaa agtatgtgaa
     1501 actaattgtt cagcagtaaa ttgtttattg agtttttggc taaataatcc agtagaactt
     1561 ctaaagtctg gaatattaga ttcagttgaa acacctgctc caccaaaaaa tacaatatta
     1621 ttgtgattag ctattaagtc ttttaagcta tttgcatcca tagttacacc tccaaattat
     1681 tatatataaa ttgtactaca tattaggtct tttatgtttt attaatttaa ataaatatgt
     1741 agtttaataa gttaaataaa gggaatataa ataaaatgta tacagagtat aaatagacaa
     1801 tatttactaa aataacaagt tgtcgtataa ttatattaaa attgttttag tgtataagaa
     1861 gggcggggga agtacgtgaa gaaaaaagca gctttagcaa cattggctat gttaccctta
     1921 ggtgtggtaa atgcacatgc tgatggagat ataggtatag tgactataaa ttatttaaat
     1981 gtaagaaatg agccaactgc cgaaagtagc atagcctttg ttgctaaaaa agatgataaa
     2041 gttttgatta aagactcttc taatggatgg tataaaataa aagctgaatc tggacaagaa
     2101 ggttgggctt catcaaaata tatagcaaaa tcaaatagtg actctttaag aacgtctaca
     2161 aataaagaaa aacaagttat ttcaaatagt ttaaatatga gaaatggagc aggtacaagt
     2221 tacagagtaa taactgtact aaaaaaaggt caaaaagtag aagtaatatc agagagtaat
     2281 ggatggtcta aaatcaaata tgatggaaga ctaggatatg tatctagctc ttatttagga
     2341 gacgtttcaa attcaactaa taaatcaaaa actaaacaag tgaatacaac ttcactaaat
     2401 gtaaggagtg gaccaaatac aagttatggt ttgttaggca agttgccaaa aggaagtaaa
     2461 gtagaagtaa tatcagaaag taatggatgg tccaaaataa agtataatgg aaaagatgcc
     2521 tatatatcta gtatgtactt atcggatgta agtcaaagta attcagacaa ttctagtcaa
     2581 agcaatgata aaaagaatac tgataaggtt gtaaatacag cttctttaaa tgtaagaagt
     2641 ggaccaggtt ctacatatag taagttggga aaagtttata aaggaagtaa agtaactgta
     2701 ctatcagaaa gtagtggatg ggctaagatt aattttaaca ataaagaagc atttgtagta
     2761 ggcaattatt tatctacttc agcagatact tcaaataata actcaaatag taactctgat
     2821 aacagttcta atagtaatgg caataattct tcatcatcag gtcaagtaaa tggtatgtca
     2881 ggtataagtg gggctaaaat tgattataaa tctttgagtt atactttaga atctcatata
     2941 agtaaacaag ttgagaaagc agcatcagga ggaaatgtga tagctccaag taataggaaa
     3001 agcactccaa gtcctgaatt tagtactttt tcagctcaaa gaacaagctc atttgtaaat
     3061 gcaagttcaa gtgatataga atattattta aatcctaaaa attttacaaa tactactaaa
     3121 ggtatgatgc agtttttaaa gattaacagt tatagagatg gtatttcaga atcaagtctt
     3181 aactcgtatc ttaatggttt atcttctagt gtatttaaaa accagggagc agcctttata
     3241 aatgctgcca agaagtataa tatagatgtt gtatatctag tatctcatgc tatgtgggaa
     3301 actgcttatg gtaagtctac acttgcccaa ggtcaaacat taacatctta taagggacaa
     3361 cctcttagta agccagttaa agtatacaat ttttttggta taggagcaat agataaaagt
     3421 gctaatgttt caggtgcaga agcagcgtat tccaatggct ggactagtgt agaagctaca
     3481 atagatggct ctgcaaagtg gatatcacaa aattacgtaa atagttctaa gtacaatcag
     3541 aatactatct acaagatgaa gtggaattat gattatacat ggcatcagta tgcaactgat
     3601 gtaaactggg ctaatggaat ttctggaatt atggaaaatt taattggtct ttatggagga
     3661 ggaagtagtt tggtttttga agttcctcaa tataagtaat acataaataa aaaatataga
     3721 ctatgggtag ttttcatttt atttaaatta tctatagtct ttttgtatat aatttataat
     3781 ctgtaaattt aatgttaaca aaacttttat gttataatat taattctaag taaatataag
     3841 tgagtaatag gtagaaatag ataaaaagtg gtaataatat atataaagat aattttgaga
     3901 aaggaggata tatatggaaa gtattaagga atatttggat aagttagaaa ttaataatag
     3961 tggtcttgga aagcaactta aagaagttta tataaataga gtagtttatt ttaaggaaga
     4021 taagatagtt tatttttatc taacttctaa agatattgtt tctcatgaac ttttagataa
     4081 atttaaagaa gaattgatgt ataaactcga ttattttaaa gatatgagga tgaagataag
     4141 atttactggt cttgaaagaa aatccaataa agatgttata aaaaaatatt ggaataatat
     4201 attgtatata ttaaagtatc tttgtccatc tattgcaggg tggtataaac aagtagaatt
     4261 tctttgttta gaagaagaat taaaaataaa acttccaaaa ggtatatttt atgagcgatt
     4321 aatgaaaaaa aatgtaactt atgtacttaa aaccgtgctt agcgaagagt tgggattaga
     4381 tttaaatata acaatagaaa aagctgttga tgaaaaggta aataaagaaa gactcataag
     4441 aatcaatgat agagaaatgg aagaaaaaat tagggcttta gaaattggaa aagttaataa
     4501 ctgtgaagaa aatgaagagg aaagctatgt cattaagtct gatgtagatg aaaatttaat
     4561 ctatggagat aatgcaaatg ctatgattga aaatatagtt gaattaaatg catcttctgg
     4621 aacagttgca gttgttggag atatatttga tgttgacact aaagaactta aaaacggaaa
     4681 aatacttatg atagcttcaa ttacagatta tactagctca ataagctgta aacttttttt
     4741 aacagataca aataaggatg gagtacttga aaatgttaaa aagggagcat acttaaaaat
     4801 caagggtgac atagtttatg atacatatca aagagaaatt agtatgatga ttagtgggat
     4861 aagaaaagaa actagaatag agagagaaga taaatcagag gaaaaaaggg tagaactcca
     4921 tgcacataca caaatgtctt ctatggatgc tatttgttct gttaaaaagc ttgtagagcg
     4981 tgcagccaaa tggggtcatt ctgctattgc tataacagac catggtgttg tacaaggatt
     5041 tccagatgct atgaatgcag gtaagtcaaa taatataaaa atactttatg gagtagaagg
     5101 gtacttggtt gaagatgatt ctctcattat agaggatgca aatgataaag aactttcgca
     5161 gacatttgtt gtatttgaca tagagacaac tggtttttct aatacaaatg ataaaataac
     5221 tgagattggt gctgtaaaaa tagaaaactt taagattgta gatagattta gtgagttaat
     5281 aaatcctgaa aaagatattt cctataagat acaggaattg acaggaataa ctaatgaatt
     5341 aattaaagac aagccaacca ttgaagaaat tcttcctaag tttatggaat ttgttggaga
     5401 tagtgtatta gtagcacata atgcagagtt tgataccgga tttatatcgc aaaaatgtag
     5461 ggaacaaggc ttgttatata ataacaaaaa agtagataca cttatgttag caagagtaat
     5521 gttgccaaac ttaaaaagac atagattaaa tgtcgttgca aaagaacttg gtattcctct
     5581 tttaaatcac catagagcag tggatgatgc tgaggctaca gcacttatat ttaataagtt
     5641 tttgcagatg cttacagaaa aaggtgcaaa aactctaagt gatgtaaaca atatacttgg
     5701 aaagattgat tacacaaagc ttagcacaaa tcatataacc ttgatagcaa aaaattctat
     5761 aggtataaaa aatttatata aaataatttc agatgcacat gtaaatcatt tttttagagc
     5821 accaagaatc cttagaagtg tattaaatga atataaagaa ggattaataa ttggttcggc
     5881 ttgtgaagct ggggtggttt ttcaagctgt aaagaaaaac gtgtctgatg aggaaatgaa
     5941 aaaaataata gatttatacg attatattga agtaatgcct atagataata atagatttat
     6001 gattgataaa ggagaagtaa aggatgaaga agagttaagg gagttgaata ggaaattaat
     6061 tgatacagcc aaaaaatttg acaaaattcc agtagctact ggggatgttc attttttaga
     6121 caaacatgaa gctgtcttaa gaaaagttct taaatattct caaggtttta aagtagatga
     6181 agaagaaact tatttacatt ttagaacaac agatgaaatg ttggaagagt ttagttatct
     6241 tggagaagaa ttggcatatg aagttgtagt tgaaaatagt aatctgattg ccgatatggt
     6301 tgaagatata aaacctatac cagatgatac ttatcctcca attatagagg gctcagatgt
     6361 ggagttaaga gaaatgtgtt acaaaaaagc caaacgaatc tatggagacc caataccaga
     6421 aatagttcaa aaaagactgg atagagaatt aaattcaata ataagcaatg ggtatgcagt
     6481 tatgtatatt atagctcaaa aactggttgc aaaatctctt tcagatggat atttagttgg
     6541 ttcaagaggc tcagttggct catcttttgc agctactatg agtgatataa cagaagttaa
     6601 tcctcttcca gcacattata tttgtgaaaa cgaagactgt aaatattcat tcttttatga
     6661 aataggtgaa tggggctcag gtgttgattt accagacaag gattgtccaa agtgtggaag
     6721 aaaacttgct aaaaacggtc atgatattcc atttgaggtt ttcttaggtt ttgaaggtga
     6781 taaggaacca gatatagact taaatttctc aggaaattat cagccaacaa ttcataaata
     6841 cacagaagaa ctatttggag aggggtatgt atatagggct gggactatag gtactgtagc
     6901 agaaaaaaca gcttttggat acgcaagaaa atatgtggaa gaaaataata taagtgtacc
     6961 aaatgcagaa gtacttaggc tttcaaatgg atgtactgga gtaaaaagaa cctcaggaca
     7021 acatcctggt ggagttatgg ttataccaga ctataaagat gtatacgatt ttacacctat
     7081 acagtatcca gcaaatgata cttcttgtgg tgttataact actcactttg attatcattc
     7141 aataagtggt agaatactta aacttgatat acttggacac gacggtccta ccattataag
     7201 gatgcttgaa gatatgactg gaataaatat aacagaaatt cctttagatg ataaagagac
     7261 tatgtctcta tttacatcta cagaagcatt aggtgttaca ccagaagaaa taaactgtcc
     7321 tataggttgc cttgctgtac cagagtttgg aactaagttt gtacgacaga tgttacttga
     7381 caccaaaccc actacatttg cagaacttgt gcgtatatct ggtctctctc atgggacaga
     7441 tgtttgggta aataatgcac aagacttagt tagagaagat atagttggat taaaagatgt
     7501 tatatctaca cgagatgaca taatgaacta tttaatattt aaaggattac ctcccaaaat
     7561 gagttttaca ataatggaga gcgtaagaaa aggtaaaggt ctaaaaccag aacacattga
     7621 agaaatgaaa aaaaataatg ttccagaatg gtatataggc tcatgtaaaa agattaagta
     7681 catgttccca aaagcacatg ctgttgctta tgtaatgaca tcatttagaa ttgcatattg
     7741 taaagttcac tatccagaag cattttatgc gacttatttt acgacaaaag ttgaggattt
     7801 tgatgcagat ttaattgtta aaggattaga tgctataaaa tcaaaaataa atgaaattga
     7861 gtcactggga aatgatgcaa ccacaaaaga aaaaggtatg ttgaccgttt tagaagttgc
     7921 tcttgaaatg tatgctagag gaataaaatt attaccagtt gatatatata aatcagatgc
     7981 aactgagttt atagttgtag gagaaaaaac attacttcca ccaatggcag caatacaagg
     8041 gcttggagga aatgcggcta taaacatcca aaatgaaaga aaaaatggtg aatttatatc
     8101 aaaagaagat ttgagaaaga gaacaaaaat atcaaaaacg gtaatagaaa ctcttactaa
     8161 tcatggttca cttgagaata tgagtgagag aaatcaattg tcattgtttt aagttagccc
     8221 aaaaagttgc aaataaaaga attttatgat atcatagtat agaaaatagt atactttgca
     8281 gaaactaaga gtgagacagc tgttctcact cttttatttt aaatatatta ttaaaaagtt
     8341 ggtttatttt tattaaatat attttattta agattatata aactaatcat ttacaattga
     8401 ataggaggaa ctttaatgaa aaaaaattta gaagctacaa ttgaggagat tgtaactaaa
     8461 ataacggatg aacatggatt tgaaatggtt gatgttgaat atgtaaaaga agcaggagaa
     8521 tactacttaa gagtatacat agataaagaa gaaggaatat ctttaaatga atgtgaatta
     8581 gttagtagag aattaagtcc aatactagat gaaaaagacc ctataaaaga aaattacttt
     8641 ttagaggtat cttctccagg acttgataga gcattaaaaa aagacagaga ttttgttaga
     8701 tatcaaggta gagatgtaga tttaaaatta tataaaccat taaatggatg taagcaattt
     8761 gaaggtgaac ttgtaggatt aacagaagat aataatataa aaatcattgc aaatggtaag
     8821 gaaatggaat ttaatagaaa agatgttgct attgtcagat tagcaataaa gttttagtgg
     8881 tatattaaat agggaggata taaaaaatga atcatgaatt tatggaagca ttagatgaat
     8941 tagtagttga cagaggtata gataaagaaa tacttataga tactatagaa caagctcttt
     9001 taactgcata caagaaaaac tttggttcag cacaaaatgt tagagttgaa tttgatagag
     9061 acaagggaga tataaaagta ttttctcaaa gagttgttgt tgatgaatca gatttatatg
     9121 atactttctt agaaatagag ttagcggatg ctagagagat aaaccctaat tatgaattag
     9181 gtgatattat agaacatgaa gttacaccta aaaactttgg aagaattgca gctcaaacag
     9241 caaagcaagt agttgttcaa agaataaggg aagctgagag agaaatagtg tataatgaat
     9301 ttatagaaaa agaaaatgaa atagttacag gagaggttgc tagagcaaat aaaaatgttg
     9361 tttatgtaaa tcttggaaga atagaaggta taatgactca aagtgaacaa ataccatttg
     9421 agaattataa agcaggtcaa aaaataaaat tctatatact tgaagttaaa aagactaaca
     9481 aaggacctca aatagttgta tcaagaagtc atccgggtct agtaaagaga ttgtttgaag
     9541 aagaagttcc tgaaatatat gaaggaatag ttcaaataaa atctgtagca agagaagcag
     9601 ggtcaagaac taaaatggca gtaaaatcta tagatgaaaa aatagaccca attggagctt
     9661 gtgttggacc aaaaggactt agagtaaaaa atatagtgga tgaacttggc gatgagaaaa
     9721 tagatataat aaaatatagt gaagacccag cagagtttat atcagcagca cttagcccat
     9781 caaaagttgt tagagtagat atagatgaag atgaaaagtc tgccttagta atcgttccag
     9841 attatcagtt atcgcttgct ataggtaaag aaggtcaaaa tgcaagacta gcagcaaaat
     9901 tgactaactg gaaaatagat attaagagcg aatctcaagc agaacaagaa atgttaaatc
     9961 ttgaaaattc caaagaaata gaagctgagg atgttgaagc tgaatttatc gaccaagaag
    10021 agttaggtac ttcagaggat aattttagtg aagaagagta aggagggatg attttgaaaa
    10081 aagtaaaaaa agtgcctcaa agaaaatgca tagcatgtca agatagagac tctaaaaaag
    10141 agcttataag aatagttaaa aataaagaag gaaaaatatt tttagaccca acaggaagag
    10201 ccaatggaag aggagcatat gtatgtaaaa gctctgaatg tctaaaaaaa gctataaaga
    10261 gtaaagcatt aaataaagct tttaaaatag atgttccaga tgaagtctat gataatctac
    10321 ttatggagtt agaagaatat gaaaaataat aaagaaaaag tactttcgtt cttagggctc
    10381 gcaactcgtg cagggaaaat agtgtctggt gatgattcaa ctttgcttga ccttaaaaaa
    10441 ggaaaagtaa atttgatttt aatcgcagaa gatgcatcaa ataatactaa aaaacttttt
    10501 aaagataaat caacttttag aaatattcca tatctatttt tttcaacaaa agaggaaata
    10561 ggatttgcaa taggaaaatc tccaagagca gttgtaggaa taaaagatga aaatttttct
    10621 aaaaagataa ttgaactaat agaaatttaa aataaaatag ggggtgatct tgtgtcaaaa
    10681 acaagagtat accaaatagc agaagaatta aatatatcaa atgaagagct tataaataag
    10741 ttagctgaat tagatataaa tgtaactgac aaagattcag tattagaagg tgaagagtta
    10801 gaattagcac ttgaaatgtt aggtgaagat ttatctcaag aaaatggaaa tgttatagaa
    10861 atagatggaa agttgacagt acaagtatta gcaacgaaat tagataaatc tccatcagaa
    10921 ataattatga agcttatgaa aatgggaact atggcaacaa taaatcaaga gataagtttt
    10981 gagattgctg ctttggctgc caaagactat ggatttgaat taacagtagc tgaaagtgat
    11041 gatacagaag ctttagaaat agaagcttta atggaaattg aagaagataa agaagaggat
    11101 ttaaaaccta gaccaccagt tgttacagtt atgggacatg ttgaccatgg taagacatct
    11161 ttactagatg caataagaaa aactgatgtg atttcaggtg aagctggagg aataactcag
    11221 catataggtg cttcagaagt taaaatcaat ggacacaaaa tagtattttt agatactcca
    11281 ggacatgagg ctttcacatc aatgagagca agaggagcac aagttacaga tatagcaata
    11341 ttagtagttg cagcagatga tggtataatg cctcaaacag ttgaagctat aaaccatgct
    11401 aaagcagcag gtgtaccttt aatagttgct attaataaaa tagataaacc aggtgcaaac
    11461 ccagataagg ttaaacagga attagcagac caaggattat tagttgaaga ctggggtgga
    11521 gaagtaatag cagttccagt atcagctaag aaaaaagaag gaatagatac acttttagaa
    11581 atggtattat tggttgctga aatggaagag ttaagagcta atccaaataa aagagcagta
    11641 ggaactgtta tagaagctga gcttgataaa ggaagagggc cagttgcaac agtacttgtt
    11701 caaggtggta ctttaacagt aggtgaccca atcgttgcag gtgttgcatg tggtaaagtt
    11761 agagcaatga taaatgcaaa aggtaagaga gtaaaaacag caggaccatc tacagcagtt
    11821 gagatattag gtctatcaga agttccacaa ggaggagacc aatttgtcga agttccaact
    11881 gataagattg ctagaagtgt agcagcaaga agacaacaaa tagttagaga tgaaatgtta
    11941 aaatcaactc aaagattatc actagatgct ctatttagtc aaatgagtga aggttctata
    12001 aaagacctta atatagttat aaaagcagat gtacaaggtt cagtacaggc agttaaacaa
    12061 tctttagaaa aattatcaaa tgaagaagtt caagttaaag taatacatgg tggtgttgga
    12121 gctgtaacag aatcagacat attacttgca gcagcttcta acgcaataat aataggattc
    12181 aatgtaagac cagttccagg tgcagaatct ctaggagaaa aagaaaatgt tgatataaga
    12241 acatatacta taatatacaa ggcaatagaa gatatacaag cggctatgac tggtatgtta
    12301 gaccctgaat atgttgatga agaaacagga aaagctgaaa taagagaaat atataaaata
    12361 tctggtgttg gaacagtagc tggatgttat gtgactaatg gtaagatatt tagaaattgt
    12421 aaagtaagat tagttagaga tagcataata atacatgaag gtgagcttgc agcacttaag
    12481 agatttaaag atgatgtaaa agaagttaat tctggatacg aatgtggtat gagttttgta
    12541 aattataacg acataaaaga aggcgatata gtagaagctt atataactaa agaagtagaa
    12601 agaaaattat aaattcgtat caaagaaaat cagttagtaa agaaggtcga tggtatggca
    12661 tcatataata gaacaagaag gatagcagaa gaaataagaa aagtcgtaag tactatgctt
    12721 ataaatggag taaaagaccc tagaataaca tctatggtga gtgtaactga tgtagaagtt
    12781 acaaatgacc taagatatgc ttatgtatat gtaagtattc taggtggaga tgaagaatcc
    12841 acactaacag gtcttaaaag tgctggagga tatataagaa gagaagtagg aaaaaatata
    12901 aaacttagat atattcctga gatagtattt aaacttgatg attcaataga aaaaggaatg
    12961 tatatggata accttataaa aagagttaat gaaaaaaatg ctcaacaaaa tgaggatgaa
    13021 aattatgatg aataatatgg atagtattat aaactatatt tatgagggtg actattttat
    13081 agttacctct catatcagtc cagatggaga caatgttggt tctacacttt caatgtatta
    13141 tacattgaag aatttaggaa aaaatgtata ttatgtactt gatgatgaag ctccattaaa
    13201 tttaaggttt ttggttgaag gtgtaaaaat ctataagtct aatgaattta atatgaagaa
    13261 ctattcttta attgcacttg attgtgggga taagatgaga atatgtgtga gtgatgaaat
    13321 caaaaataat gcatcaaaat taatctgtat tgaccatcat gctagtaatg attcatatgg
    13381 agatttaaat tatgtagata ttgatgcatc ttctacatgt gaactagttt ataatttatt
    13441 ggttagattt cagcaaacaa aagatattaa tattataaat gaagatatag caacatgttt
    13501 atatacaggt cttgtaactg atactggaaa cttttcgtat tcaaatgtac atgcaagtag
    13561 ttttgaaatg gcaaaaaatt tacttgtttt aggtgcacaa aaaaatacta taatacaaaa
    13621 tatatatcaa agcaattctt ctgattatta taaattatta ggagaagctc taaaaggttt
    13681 ggagatattt gattcaaagg tatctagtat agttttaact caagacatga tgaatagaaa
    13741 taatattagc tttaatgatg ttgatggaat aacaacatat acaagagata taaaaggtat
    13801 agaagttgga attttattta aagagaaaaa acaaaatgaa attaaagtga gttttaggtc
    13861 aaaaaattat gttgatgtaa gtgaaatagc caagttattt ggcggtggtg gtcatgtaag
    13921 ggctgctgga tgtactatta gagatagtat tgataatgca aagaaaatgg tactagaggc
    13981 agtgttaaaa tcaatttaag gtgacagtaa aatgaataag attataagta tattgaaacc
    14041 cacaggtatg acatctcatg atgttgtaag tagagtgaga aagattttaa atataaaaaa
    14101 agttggtcat acaggtactc ttgacccaga tgcttcaggt gttttaccaa tctgtatagg
    14161 gaaagctaca aaagtatgtg aagttatttt aaacaaagat aaatcgtata tatgtgagct
    14221 tactttaggt ataagtacag atacttatga tgcttcaggt gaaattttaa aaaaagttga
    14281 tgattttaaa tttagtaatg aagacataga aagagctttt gatacacaaa gaggagagat
    14341 aaatcaactt cctccaattt attcagcttt aaaggtaaat ggaaagagaa tgtgtgattt
    14401 agtaagaagt ggtagacaat ctgaaattac tttaaaaaca agaagagtaa atataaaaga
    14461 cataaaaata ctaagtataa aaggtaataa agtaatgttt tatgttgagt gttctaaagg
    14521 gacttatgtg agaagtattt gccatgatat aggagaatat ctgggttgtg gagctcatat
    14581 gtcgtttttg aatagaacat cttctggtaa atttgactta gataattcaa taacattaga
    14641 agaattagaa ttattttatg aaaataaaac tttagacaaa tacttatatg atatagatta
    14701 tgtccttgat tcgtttaatt atgttgtatt aaaccctaat gctataaaat attatagtaa
    14761 tggtggctca atagatgata aaaggttttt aaaaaataat ttcgataaag atgatgaagt
    14821 tgtaagagta tacagtacag ataatttctt aggattagga aagttatcaa aacacaataa
    14881 tacaataagt gttaaaagtg ataagatgtt catataacca atgtatttaa aaaaacaaca
    14941 aaagagatag tactataata acttctatag tctatctctt ttacttatgc tataatagtt
    15001 tgtgaataag ttaagagtaa attatatttt agcggggaac atttattatg gtgattataa
    15061 aatctataaa agaaatagca aacataaaag aaagtgtcat taccatagga aattttgatg
    15121 gtgtacacaa aggacatcaa gttcttatag gaaaaacagt tgaatatgca aaaaaagaaa
    15181 atatacagag tattgtattt acatttgcaa atcatcctgt aaattacttt agaccaaact
    15241 caacaaaaaa tattataagc aatgatgata aaataaaatt attagaagat ttgggaatag
    15301 atatagttgt aatcatacca tttgatgagt atatgactaa aatacctgca aaggatttcg
    15361 tagatgagat actagtaaaa aaattaaaag ctaaaaagat agtaattgga catgacttta
    15421 catttgcaag ggcaaaagaa ggcaatgtta atctattaaa aagcttagaa catgaatttg
    15481 gttttgaggt agaagtaata aaacccatca aaatcaatga tgtaagagtg agtagtactt
    15541 atattagaag tttagtatcc cagggtgata tggctaatgt caaggaatat ttgggtagaa
    15601 attacaagtt agaaggttgt gttatacatt ctaaacacct tggaagaact ataggattcc
    15661 caacggcaaa tattgacctt aagaataata tgctcgttcc aaagagaggt atttatgctt
    15721 ctattgtaca tattggagat gagacttatt ttggtgctac aaatgtaggc tataacccaa
    15781 cagttaatgg aaaaactttg tctattgaaa caaatatttt agagtttgat agagatattt
    15841 atggggaaaa tataattgtt gaatttttgg aaagaataag agatgaaaga aagtttaatt
    15901 caatagatga cttaaaaaat caattgtata atgatacaaa ttatgtgtat gaaaactatg
    15961 tttgcaaaaa caagtaatgt atgataaaat gattaaggaa aataaccatt actcggcatt
    16021 tgaatactcc aatcaatgct tagtaatagg cgtttctaaa aaaatacgga ggtaatctaa
    16081 atgataaaca aatcagaaat aataaaagaa tatggaagag ctgaagggga tactggttct
    16141 ccagaagtac aaatagcttt attaacagct agaataaacg agttaaatgg acatttaaaa
    16201 actcataaga aagaccacca ttcaagaaga ggactattaa aaatggttgg tagaagaaga
    16261 aacttattag cttacttaaa ggaaaaagat cttgaaggat atagagcatt aatagctaaa
    16321 ttaggattaa gaaagtaatt tcattcttta taaaaaagca ggtatagtgt atacctgctt
    16381 ttttatagtt aattcattat aaagtaaaat agttaataat aaaagtagtt tatatgttat
    16441 aaaaaattat aaaatgaata atattgggta agaatattaa aatagtatac aaatacataa
    16501 tcaatttaaa aggaaagaca ggataaatat agaaatttta taacaggata attagttgga
    16561 ataaggagga tattattttg aaaataaagt tagtatgcga tagtttgtgc gatattccag
    16621 atgaaatatc agaaaaagat tatatagaga tagttccact tacagttatt tttaatgata
    16681 gggaatatat agaaggagta gatataaaga aagaagagtt ttataaaaaa gtaaaagaaa
    16741 tcaagcaaat acctaaaact tcacaagcta catatatgga gtttaaagaa gtgtttgata
    16801 agttcataac agaaggttat catataatct gtatgacagg ggctgcaaac gcatcaggaa
    16861 cttttcaaag tgctatgata gctaaaaatg atgtaaatga aaatgaaaaa atacatatat
    16921 ttgatactag aaatttatct ctaggtagtg gacaatatgt tataaaggca tgtgaacttc
    16981 ttgaagaagg actgggattt gaagaaatta tagatgaatt agaaaatact cgcagtagtg
    17041 taagattact ttttgcacca tatacacttg attttttaaa gcagagtgga agagttccag
    17101 ttgcaactgc tttgatagga aatatgttaa atataaagcc tatatttttc tttgataatg
    17161 gggaagctaa gttggtaaat aaggttagag gaataaagaa tatagcaagt aaactggtcg
    17221 atataatttt agaaatgaat gaagggtctt tagaaggaaa aatcgtcaca ataggttgtg
    17281 gagataattt acacgattgt gaaatattaa aagatgaagt aaataaaaga ataaaagcaa
    17341 gaagagtatt atttactcga ggaggagttt ctatttgctc tcatacaggg ccagatatat
    17401 tggctataag ttgttctaat tagtagtaat ataaggtttt tgcatatata tgtgaagata
    17461 attttatatt gttaatttat agtatttttg ataatatagt aaatagagtg atatacgtgt
    17521 tttttaataa gaggaggaca agcattaatg tttgaacata aaatatttaa aatggatttt
    17581 gcaggaagag aactttctgt agaaatagga aagatttgtg aaatggctag tggtagttgt
    17641 atagttcgat acagtgacag tatggtaatg gtaaatacaa ctaaatcagc caaacctaga
    17701 gacggaatag acttttttcc tttaagtgtg gattatgaag aaaaattata ctctgtaggt
    17761 aagatacctg gaggtttttt aaagagagaa gggaagcctt cagaaaaagc tatacttaca
    17821 tcaagattga tagatagacc aataagacct ttattcccaa aaggatttag aaatgatgtt
    17881 caggtagttg caacagttct atcagtagac caagattgta caccagatat agtagcaatg
    17941 ataggttctt caatagccct ttctatatca gacataccat ttaatggacc aacaggttca
    18001 gtatgtgttg gccttgtaga tggtgcattt gttgtaaatc caaacgcaga gcaaagagaa
    18061 aaaagttcaa tgcatctagt tgtttcaggt acaaaggaag caataatgat ggttgaagcc
    18121 ggagctgatg aagttccaga tgaggtaatg cttgatgcta ttttatttgc tcaccaagaa
    18181 ataaaaaaaa tagttgagtt tatagaaggt atagttgcag aagttggaaa agaaaaaatg
    18241 cctgttgaat tataccatgc aggagaagaa ataactcaat tggttcgcga atttgcaact
    18301 gataagatga aaaaagcagt tcagactttt gaaaagttag agagaatgga aaatatggat
    18361 agagttaaag aagaaacttt agctcacttt gaagaaactt tagaagattt tgaagatttt
    18421 gttggagata tagaagaagt attacaagat ataataaaag aagaagtaag aaaacttata
    18481 gtacatgaaa atgtaagacc agataataga aaacttgaag aaataagacc tatatggtgt
    18541 gaaactggca tgatacctag agctcatggt tcagccatat ttactagagg acaaactcaa
    18601 gtacttaatg ttgctacttt aggtgcttta ggagatgttc aaaaattaga tgggttagat
    18661 gaagaagaaa acaagagata tatgcatcat tataatttcc ccgcatatag tgttggagaa
    18721 gctagaccat caagaggacc aggaagaaga gaaataggac atggtgcttt agcagaaaga
    18781 gctttattac cagtaatacc atctcaagaa gaatttcctt atgctattag attagtttct
    18841 gaagtattaa gttcaaatgg ttctacatct caagcaagtg tatgtggctc aacattatct
    18901 ttacttgatg caggtgtacc tataaaagat atggttgcag gtatagcaat gggacttata
    18961 aagcatgatg gtaaggttgc agtgctttca gatatacaag gtatggaaga ccatttaggc
    19021 gatatggact ttaaagtagc tggaacagag tatggtataa cagcaataca aatggatata
    19081 aaaattgatg gtatagataa agaaattcta caacgtgctt taaaacaagc taaagagggt
    19141 agaatccata tactaggaga gatgagaaaa actatatctc agccaaaacc agaactttca
    19201 ccatatgcac ctaagattgt taaaatgcaa ataaatcctg acaagataaa agatgtaata
    19261 gggcctggtg gaaagataat aactaagata atagacgaaa ctggagttaa gatagatatt
    19321 gaacaaactg gggaagtatt tatatcaggt attgagattg atatgattaa gaaagctcaa
    19381 gaacttataa ataatatagt agttgaacca gaagtaggaa aaacttataa aggtaaggta
    19441 agtagaataa tgaactttgg agcatttgtt gaaatacttc caggaaaaga aggattactt
    19501 catatatctc atatagctca tgaaagagtt gccaaagttg aagatgtatt gaatataggg
    19561 gatgaagttg aagttaaagt tacagagatt gatgaaaaag gaagagttaa tttatcaaga
    19621 aaagttcttt taccaaaacc agaacataaa aataaataac ttattatata attaaaagca
    19681 aagtcttagg attttgcttt tttaaataca aaaaagtaga taattttaaa atgatagtta
    19741 tggatggctt atagttcttt ttctgtaagg ctttactctt ataaagaagc tatttaaaag
    19801 ctgattaata gaaagcttta ttgatatata tagaatcact taagttatat ttagtgcata
    19861 aacagagctt atttttaata gtattaaata agaaacaatt tgtacataat tagggggagt
    19921 tttaatgata atgatggtac ttaataagag taagctcaaa aaaatcataa tactaatttt
    19981 agtagtgtta ttagtattat caggaggggt aatggtatta aagttattta acaacaagcc
    20041 agtatttaat ttatttaatg gtgtagattt accatatgaa agaggtacaa aggataaaag
    20101 tggatatgtt gcattaacct gtaatataga ccttggatgg gaaacagagt acgtagaatc
    20161 cattttagaa acacttaaaa aagaaaatgt aaagattaca tttaatgtaa ctggaaaatg
    20221 ggcagaaaag aataaggatg aattattaaa gataaaaaaa caaggacatg aaataggtaa
    20281 tcatggatat aaacatttag attattctac attatcatat gaagacaatt atgagcaaat
    20341 agagacctct aaaaagataa ttgaagaaat tataggtgaa aaaactaaat tttttcaagc
    20401 accagcaggt tcctttggtc cagaaacagt aaaggctgct aaagcattag ggtatacttc
    20461 tatcaaatgg gatgccgata ctatagattg gaaatacaaa gaccaaccag aggtaatcat
    20521 tgatagaatg aagaaaaaag acattaaaga tagtagtata atcttaatgc atccaaccaa
    20581 cgcaacgaca aaatgtatag atgatattat agctattgta agagaaaaag gactaaaacc
    20641 agggaaactt agtgatgtat ttaagtagac aaagtataaa tatttggtta agattgtata
    20701 agttgtagtt gaattaatta aaagactgac ttataatgta atacatataa aaaataaata
    20761 taattaaaac atagtgtttt caataatcca atatgaaaca aagaataaat tgttaggaga
    20821 atgtaatgta taagacaaaa atactggaaa atgggcttac aataattggt gaagaaatac
    20881 catatctaaa gtcaataaca ttaggtattt ggataaatgc aggctctagg atagaagaag
    20941 cacaggtgag tggaacttct cattttatag agcatatgat gtttaaagga accaaaaaca
    21001 gaacatcaaa agagattgct agttcaatag ataatttggg tggtcaaata aatgcattta
    21061 caagtaaaga atgtacatgt tattatgtaa aactaataga tgagcatata gatacgggaa
    21121 ttgatgtatt gagtgatatg atactaaatt ctaaatttga taaaaatgat atagataagg
    21181 aaagacttat aatattggaa gaacttaaga tgtatgaaga ctctccagat gacttatctt
    21241 atgatttatt agtggaaaat atatatgcta atgatggatt aggaatgaat ataattggga
    21301 caaaagaatc tctatacaat ataactagag aatccatgtt agaatactta aataagtatt
    21361 atattccaaa taacgctgtt atttctatag ctggaaactt taactttgat gatatggtgg
    21421 agaaaatcaa atcaaaattt ggacactggg aaaagaaaaa tttgagtata gacataagtg
    21481 aggctaagtt taatccttgc tttatatcaa aaaataaaga tactgaacag gtgaacttag
    21541 ctatctgcct aaaaggtatt ccttttgaaa atgatgaaga agtctactca atggcagtgg
    21601 taaataatat tttcggtgga agtataagct caaggctttt tcagaaaata agggaagaaa
    21661 aaggtttagt atattcaata tactcatcac agacattata taggaaatgt ggtgaattag
    21721 gcatatttgc aagtatgagt acagaaaatt tacaagatgt atataattta ataaagaaag
    21781 aaattgaaaa tataagagaa aactatctta cagaaaaaga aatatctgaa agcaaggaac
    21841 agttaaaagg aaattatata ttggatttag aaagtactag cagtagaatg atgtctactg
    21901 gtaaatctat gttacttagc aaaaaagtaa aaactacaga tgaaatactt gaatgtataa
    21961 ataatgttaa tattaattca ataaagaagg ttgttgataa ggtattcaat atcgaaaata
    22021 ttggtacatg tatagttgga agagatgtag aaaagattct acacttggat taatattgaa
    22081 aaaagtaaat ctctggaggt ataaaatatg aatttatctg aaatagcagg taaggaaata
    22141 gttaacttgg tcacaggaga aagattgggg gtcattggag aatgtgacct tatactagat
    22201 gaaactacag gcggtatatt agcgcttcta attccaaagg aaaaaggatt ttttgccaga
    22261 agaaaagata agtcattttt agaagtacca tggagaaatg taaaaaaaat aggtaatgac
    22321 atgataatta tagaatatga gggatatatt taggaggaaa aaatgagtat tttagtgcaa
    22381 aagtttggtg ggacatcagt agaatcatat gaaaaaatga atgaagtatg taagatagta
    22441 aaagcataca aaaaaaatga tgaagaatta caattagtat tagtagtttc agcaatggga
    22501 agaaaaggag ctccatatgc tacagataca cttataaatt tgtgtagtgc agtaaatgat
    22561 gagccaagta aaagagaact tgatttaata atgtcttgtg gagaaataat atcaggaaca
    22621 attcttgcta atttattgaa tgcacagggg atagattctg ttttctttac tggtcagcaa
    22681 gcaggtataa ttacaagtga tgaatattct aatgcaaaga taaagtatat aaatcctaaa
    22741 aaggtaaaaa gagctttaga tgatggtaag gttgtagtta tagctggatt ccagggtgta
    22801 acagatgatg gagaaataac tactcttgga agaggaggaa gcgatacatc agcagttgct
    22861 attggtaaag ctttagaatg tgaaacagtt gaaatttaca ctgatgtgga tggtataatg
    22921 actgcagacc ctagagtaga accaaacgct aaagtattga gttttataga ttatgaggaa
    22981 gtattccaaa tggctgacaa aggtgcaaaa gtaatacatc caagagcagt acagttggct
    23041 aaaagtggaa atataacttt agccataaag aatacaatga atccaacttt tgaaggtaca
    23101 aaaataggtt ctttatgtag acacttagaa gagaatatag aatatgaaca agaaagagat
    23161 tttaaggttg ctgttgcaaa taaagatagt gtagcacaag ttaagattaa atcagcagaa
    23221 gaagtgttta cagaagtatt aaatgagata gaaaagaaac ttataactat agatatgata
    23281 aatttcttta tatcagaaaa agcatttgtt gtagaagatg ctgatataaa atcattaaag
    23341 gaaatactag ataaatttga attagattat gaagtaaata gagactgtgc taaggtaaca
    23401 ttgatatgtt caagaattgc tgatgaaatg tctggtatca tgtcaaaggt agttagagga
    23461 ttatcaaaag caggggtatc tcttttacaa acatcagact caaatatgac tatatcttgt
    23521 ttagttagtg aagaagatat gcatacagca gtacatgcta tacaccaaca attttattta
    23581 aaataaactg tataatacaa ttatttttta atcaaattta agaatttcat atatatctta
    23641 atatatttga tttatttggt aaaactaaat taaatataat tttgatagga gtttttttat
    23701 gaaagattat agagaagata atgaaagatt tgataaagat aattgtagtg aagaatctag
    23761 ttgcgaagag agagaatgta taaagcaatt tggtaccaat gaaatgccac ctcaacctcc
    23821 taaagatatt caatgtataa ctataattgg agaaattgag ggacatttta taggaaatcc
    23881 tcaaaaaaaa gctacaaagt acgagcatat aattccaatg ctatactcaa ttgaggaaag
    23941 caatgatgtt aaaggagtgt tagttgtttt aaacactgtt ggaggagata ttgaagcagg
    24001 tttagctatt gcagaacttt taaacagtac atctaaaaaa gtagttactc ttgttctagg
    24061 aggaagtcat agtataggtg ttcctcttgc tacagcagga gattactcat ttatagcacc
    24121 aacagcaacc atgattatac atccagtgcg aactactggt cttgtgattg gtataaatga
    24181 gacttttgaa tattttaaaa agatgcaaga tagaataatt caatttataa taagaacatc
    24241 aaatatcaaa aaagatgtac ttgaaaaact tatgcatgaa aaagatgaac ttgtaagtga
    24301 tgttggtagt gtattaatag gaaaagaagc tgtggattat ggtcttatag atgaagttgg
    24361 aggactaaaa gaagcactta aaaaattaag agagcttata aaagaatctg aggaagaaaa
    24421 aaataatgat taaaatttgt ttaaataata aaatttatga tataatgatg taataaagta
    24481 ggagtgttag tgttaaggca ttagcacttt ttgctgtgaa ggggtaaaac ttgctaaaaa
    24541 taaggagtga tattaatggc cacgaaaaag aagaaaaaaa agaaggtaaa taccttaagt
    24601 tttaatgcag aatatcataa tttaataaca atatttatag gtgtattttt attatatagt
    24661 ttaaattcta attctatggg atggatacct gtgttaatgc aaaatctttt taagggacta
    24721 tttggagggc tttcaatagc aataccattt atagtaatta taacaggact tttaggattt
    24781 tttgatggaa atgaatatat atataggcta agaaagacta aactatacta cataattatt
    24841 ttatttatat ttgtatttta tggattatta aatgcaggaa ctcttcctgt agatagccca
    24901 ctaaaaggta acatgtttga tgatgttatg aaattgggtg taagtggaca aggtagtggt
    24961 ttgatagcaa caactattgc gtattatatg agtaaaatat ttggtatagc aggtggatgg
    25021 ttaataagta tcttcgcact tatactttct gtaatgttta tatttaatat ttctataaaa
    25081 gatttggtgt caaatgcaaa atcaaaatct aaagcatcta aagacagcaa tttaacgttt
    25141 aaagataaaa ttgcaaatat gaaaaaatct gcaatagata tgatgactga tgaagtagat
    25201 gatactacta taaataaaaa accaggtttc tttaaagggt taatgtcaaa aggtcttaat
    25261 aatgatgatg aagaagatga atattttgaa gaagaaaata gtgatggtgt agatgataaa
    25321 actataaaaa tagttggatt taataaggca gaagatgagt atttagaaat attagaagga
    25381 actcaaagta tgccagagtt agatgtttta aaagaattac aaaaagctac caatgaaaat
    25441 ccagttgtag atactaaacc agaaaaaaaa gttgatatag ctaaatctaa tttaaatata
    25501 gaaaaaactc aacctatgag tattgtagca gagccagtta atgaggatta ttcaaactat
    25561 aaaaagccta gcattgagct tttaaataaa gtaaataaga agtctgatga aaatggtaaa
    25621 aagaaagtgt tgaaaaatgc ttctctttta gaaaagacac tatcagattt tggagtggaa
    25681 gcaaaaatta atcaagttac agttggacct actataacaa gatatgaaat acaaccaagt
    25741 ccaggagtca aggtaagtaa aattgtaaat ttaactgatg atatagcttt aagtttagca
    25801 gctaaaagta taagaattga agccccaatt cctggtaaaa gtgcaattgg aatagaagtt
    25861 ccaaatgaag aagctcaaat ggttggtgtg agagaagttc tagaaagtga agaatttaat
    25921 aattttaact ctcctcttgc aatgggactt ggaaaagacg tagcaggaaa aattataatt
    25981 ggagatatag gtaagatgcc acacttacta atagctggtt ctacaggttc aggaaagagt
    26041 gtgtgtgtaa atacattaat aagtagtatt ttatataagg caaatcctga tgaagtaaaa
    26101 ttactattaa tagacccaaa agtagttgaa cttgcaaatt ataatggaat accacatcta
    26161 ttgataccag tagttactga ccctaaaaaa gctgcaaatg ctttaaattg ggcagttaca
    26221 gagatgaata gaaggtataa attatttgca gatgctcaag taaaagatgt aacaagttat
    26281 aatgagaaag cggaagaaaa acttcctaaa atagtaataa taatagatga gttagcagat
    26341 ttaatgatgg caagtgcaaa tgatgtagaa gattatattt gtagattagc tcaaatggca
    26401 agagctgctg gaatgcactt gattgtagca actcaaagac catctgttga cgttataaca
    26461 ggggtaataa aggctaatat accatctaga atagcatttg cagtatcatc tcaaactgat
    26521 tcaagaacaa tccttgatat gggtggtgca gagaagcttc taggtaaagg tgatatgtta
    26581 ttctatccac ttggtgcagc aaaaccagtt agacttcaag gggcattcat atcagagagt
    26641 gagtcggaaa aagtaattga ttttgtaaaa agtcaggtta aagatggaat aaaatatgaa
    26701 gaagatataa ttgaaactat atctaaggtt aatacttcaa agagtagtga tgaggatgag
    26761 tttttgagtg aagcgataga atttgtcgtg gaaagtggtc aagcatctgc ctctatgcta
    26821 cagagaagat ttaaaatagg attcaataga gcagctagac ttatagattc tatggaagaa
    26881 agaggcataa ttggagcaag tgagggaagt aaacctagaa aggtattaat aagcaaacag
    26941 gatttgcaga atttagaagg tgaatagaat tgtcagttat aaaaatagaa aaagcactta
    27001 agctagataa agtaatattt gtagatgtga gaactgaagg agaatatgaa gaagaccata
    27061 tattgaatgc aatcaatatg cctcttttta aaaataatga acataatgaa gttggaacaa
    27121 tttataagat gcagggaaaa catgaagcaa tacaaaaagg gtttgactat gtatcgtata
    27181 aattaaagga tatatattta caagcagcag aacttgcatt aaattatgat aatattgtca
    27241 tatattgtgc aagaggcgga atgagaagtg gaagtatagt aaatttatta tcatcattag
    27301 gtgtaaatgt atatcaactt gaaggtggat ataaggctta tagaaatttt gtattagagt
    27361 atctaaggca tgtaatggat acaaaaaact ttgtatctgt tcatggattg actggtacag
    27421 gaaagactga tttgttacat ttattagatg aaaaaggaat agatgtactg gatttagaag
    27481 gcatagctaa aaacagtggt tctacctttg gttttataac ttttgataaa aagccaccat
    27541 ctcaaaagaa ctttgaaaca aaactatttg aaagtgtatt tttttcgaaa gagaattata
    27601 tatttgtcga gagtgaaagc aaacgagttg gaagtgttac agttccaaat gaaattttcg
    27661 aggcaatgac acgagaagga catcatatat tattagaatg tgatattgaa aatagagttg
    27721 atagactttg tagagattat atttatgata aagatgaagg taatatattg attttaaaag
    27781 aatgtataaa caagtttaga aaaagattgg gtaataaagt agtagatgat tatattgatt
    27841 tattagaatc aggtgaatat aaagaacttg taaaaagata tcttttagaa tactacgacc
    27901 cactttatat gcattcagta gagaaatata agtatagtaa gattataaat tttaatgata
    27961 cacaaaaagc tttagaagat gttatagact tatatgagag tatattggaa ggagaaagtg
    28021 aatgttaaaa atagcattgg aatcattagg atgctcaaaa aatttagtag atgcagaaat
    28081 aatgatggga attttaaata acaaaggcta taaattaatt ggagattttg aagaagctga
    28141 tgtaattata gtaaatacat gtgggtttat agagtcagct aaacaagaat ctatagatac
    28201 gataataaac ttttcagagt taaaaaaaac tggcaattta aagttgttga tagtaacagg
    28261 gtgtctagcc caaagatatt ctgaagaatt aaaaactgaa attcctgaaa tagatgctat
    28321 agtaggaaca ggaagctatc aaaatataga taaaatactt aaagaattaa gtgaaatgca
    28381 tcaaattgta agccttaatg atatagagtt tgtatttaat gaagatttac caagatatat
    28441 atctacacct agctatatgg cctatttaaa aattggagaa ggatgctcca ataattgtac
    28501 ttattgtata ataccaaaac taagaggtaa atatagaagt agaaaatttg aagatataat
    28561 taaagaagct aagaaattag ctgaatctgg agtaaaagaa cttgtggtta tagcacaaga
    28621 tacgacaaag tatggttttg atttatatgg aaaagaaaga ttatcagaac ttttagaaga
    28681 acttgcaaaa atagatggat ttaagtggat aagggttatg tattcatatc cagaatctat
    28741 aacagaggaa ttgatacaag ttataaagaa atatgataat atatgtagtt actttgatat
    28801 gccaatacag catgcaagta acaatatttt aaaattaatg aatagaaaaa ctactaaaga
    28861 agatatatta aataaaatca atctgataag aagtaatata ccagatgcta tacttagaac
    28921 tacgataata gtagggtttc ctggtgaaac agaagatgac ttcaaacaac ttgtggactt
    28981 tgttgaagag gttaaatttg atagacttgg tgcatttgca tattcaagag aagaagatac
    29041 accagcagat agattgccaa accatataga tgaagaagta aaaatacaaa gaagagatac
    29101 tttaatgatg attcaacaaa aaatatctga agaacttaat gacaaaaaga taggaaaaac
    29161 ttatgaggtg ttaatagaag aacagataga agataatgta tatacaggaa gaacacaagg
    29221 tgatgcggaa gaaattgaca gtatagtgta tgtaaaaagt gtagacaatc ttgaagtagg
    29281 agaatttgta agtgttcaaa taaatgatgc aatggaatac gatttaatgg gagatgtttt
    29341 atatgaactt gcctaacaag ttaactttgt ttagaatatt tttaatacca gtatttgttt
    29401 taattatgtt gctcaatgta ccaaataaat ttttaatagc ttgtattatt tttataatag
    29461 cttcaattac tgatgcttta gatggaaaaa tagctagaaa atacaatcta gtcactgact
    29521 ttggaaaatt tatggaccca ttagcagata agttattggt tatttcagca cttacatgta
    29581 tgatagaaga ccatcttgta agtagttgga tggtaattat aatagtggct agagagctta
    29641 cagttagcat attaagagct atagctgctg ctgatggaaa agtaattgct gctggaaaca
    29701 gtggaaaact taaaacgata actcaaatgg tatctatagt gtttttattg ttaggcgctc
    29761 agtttgaaaa tgtattgata ttaaatattg gtgaaattct cattcttata gctacactac
    29821 ttacattata ttctggatgg gaatatctat ataaaaataa agagcttttt atgtcttcta
    29881 agtaaaaaat tatgctttaa taaataaaaa ctaatgatta ataaataaaa aataacaact
    29941 taaaatttta aaaatgtaaa aaagggtata ctattatagt atacctcttt tcattttaag
    30001 aatattttct tttgtgtata taattattaa taatggttta taaataaaat gtaatgaata
    30061 aaaatataat cattagtagc gtttttaaat tgactaggtt tgatttatgt tatataatta
    30121 atatataaca agagaacaaa tgtttgtaga taaatgaagg gatggtatag agatgagtgt
    30181 agatcaagaa aaattaaaag cgttgaatga agctttaggt aaaattgaaa aagattttgg
    30241 taaaggttca gtaatgaaat tgggagaagc aacatctatg tctatagatg ttatatcaac
    30301 aggagcgatt ggtttagaca tagctattgg tataggaggt ctacctagag ggagaatagt
    30361 tgaagtatat ggtccagaat cttctggtaa gactactgtt gcgcttagtt gtgtagcatc
    30421 agctcaaaaa gatggaggaa tagctgcatt tatagatgca gaacatgcac ttgacccagt
    30481 atatgcaaaa gctttgggtg tggatgttga taacctaata atatctcaac cagatacagg
    30541 tgaacaggct ttagagatag cagaggcatt gataagaagt ggagcgatag atataatagt
    30601 aatagactca gtagcagcat tagttccaaa ggctgaaata gatggagata tgggtgattc
    30661 tcatgtaggt ttacaagcta gacttatgtc acaagcactt agaaagttaa ctggttcaat
    30721 taaaaaatca aattgtgttg ctatatttat aaaccagtta agagagaaag taggaataat
    30781 gtttggaaac ccagaaacta ctactggagg acgtgcacta aaattctatt catcagttag
    30841 attggatgtt agaaaaatag atacaataaa acaaggtgat aaagttatag gtagtagaac
    30901 tagagttaaa gttgttaaaa acaaagtagc accaccattt aagcaagctg aatttgatat
    30961 aatgtatgga gaagggattt caaaaattgg agacctttta gatatagctg ctgatgtaga
    31021 tatagttaaa aaatcaggtt catggtatag ttacaatgat actaaacttg gacaaggaag
    31081 agaaaatgtt aaaaaattct tggaagataa tttagattta actactgaaa tagatgagaa
    31141 agttagagca ttttacaatt taaatgaaga acatgaagaa tcaggtactt cagtatcaaa
    31201 agaaattgta gaagaataag taaaaataca aaaaaataac tagtaattaa agacatgcca
    31261 cgctccctat tagcttgaca caatgttgaa aaaattataa aattaagtga ggataaaata
    31321 tctaaattta attttaatta ttaggaatat tttttaatca tagtgatgac attttgggat
    31381 tattaacgaa ggaggtggat gtcatagata gtgtagtaat gattgtaata ggtgcagctg
    31441 ttggtattat agctggatat tttgttagaa aaaatatatc tgaagcaaaa attggacaag
    31501 ctgaaaactt ggccaaagaa ataatagata aagcacacca cgactcagaa acagtacaaa
    31561 aagaaaaatt actagaagct aaagaagaga tccataagtg gagaacagaa gctgaaagag
    31621 aaaacagaga aagaagaaca gaagtacaaa agtatgaaaa aagagtagta caaaaagaag
    31681 aagtcttaga tagaaaatta caaaatttag aatctaaaga agtaaaccta agtgaaaaat
    31741 taaaggtagt ggaaaagaaa gaagaagaag tcgaagtaat aaaaactcaa cagttagaaa
    31801 aattagaaag tatatcagga attacttctg ataaggccaa agaaatcatc ttaaccaatg
    31861 cggagagaga tgtaagacgt gaaatgtcta taatgataaa agagatagaa agtcaagcta
    31921 aagaagaagc tgataagaaa tcaagagaaa ttataggata tgcaattcaa aaatgtgcag
    31981 cagatcacgt agccgaaact actgtaacag tagtaagctt gcctaatgat gaaatgaaag
    32041 gtagaataat aggtagggaa ggtagaaata taaggacttt agaaacctta acaggtatag
    32101 accttataat agatgacact ccagaagccg taattttatc aggatttgat ccaataagaa
    32161 gagaaattgc aagaattgct ctagaaaaat taatagcaga tggaagaata catccagcta
    32221 gaattgaaga aatggttgaa aaggcgagaa aagaagttga caatattata aaagaatatg
    32281 gtgaacaagc tgcatttgaa actagtgtac atgggattca tccggaatta ataagattat
    32341 taggtagact taactataga actagttatg gacaaaatgt acttaaacat tctatagaag
    32401 ttgctcatat agctggtata atggctgctg aaataggagc agatattaga ttggctaaga
    32461 gggcaggttt attacatgat ataggtaagg ctgtagacca tgaaatggaa ggtacacatg
    32521 ttgaaatagg tatggattta cttagacgtt ataaagagtc taaagaagtt atacatgcca
    32581 tgagtactca tcatggagat tacgaacctc aaacagtaga ggctgtatta gttacagcag
    32641 cagatgcaat atcagctgct agacctggtg cgagaagaga aactttagaa gcatatataa
    32701 agagacttga aaaattagaa gaaatagcta attcatatga aggtgtagaa aaatcatttg
    32761 ctatacaagc tggtagagaa ataagaataa tggtaaaacc agaagctata aatgatgaag
    32821 aaatacatct attggctaga gatatgacta agaaaataga ggatgaactt gaatatcctg
    32881 gacaaataaa agtaagcata ataagagaaa caagagctat agagtatgcg aagtaaaaga
    32941 aataatatct aaataataag aaaatatttt tagaaattta aatttgccac aagatatgat
    33001 aatatatctt gtggctttta aattgtttaa gtttaatttt atataatgca aaaataatct
    33061 aagtgctaaa agtgctactt acaaaagatt aataaaataa agctttatat atgtattatt
    33121 tatataatta tccaagtttg tttgatataa tgaagttgta aaatcaatac tttactacaa
    33181 aataaatagt aaatactaca gtaaaatcta aaaataggat ataatagtat taatataaga
    33241 agattataaa taaaacctgc taatgaaaat agattatttt tagcaggttt ttaaattttt
    33301 tataataaat cttattttta gtcaacaaga tttatttttt ttaaaacttt acttact
//
LOCUS       DAELXX010000031        33222 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.031, whole genome shotgun sequence.
ACCESSION   DAELXX010000031 DAELXX010000000
VERSION     DAELXX010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33222
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.031"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            357..2213
                     /locus_tag="KPH29_001440"
     CDS             357..2213
                     /locus_tag="KPH29_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBG1467059.1"
                     /translation="MDIHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGIYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYRGGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIIDNIIYSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALINKIFESDKGVVENKKVVTFELNVSINDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSENNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    2296..2536
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    2602..2837
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2922..4139
                     /locus_tag="KPH29_001441"
     CDS             2922..4139
                     /locus_tag="KPH29_001441"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBG1467060.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRSIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            4253..4747
                     /gene="cdeM"
                     /locus_tag="KPH29_001442"
     CDS             4253..4747
                     /gene="cdeM"
                     /locus_tag="KPH29_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HBG1467061.1"
                     /translation="MENKKYANGGYSEDWYERGESTAKWFQNDREEYERAAYDEDRER
                     RGSNCGCSDSGGNRPRNCERFRREAEIREREAREAFCESSERKKEALAYECEARKLWE
                     EAEKYWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCN
                     CCRK"
     gene            5012..6277
                     /gene="hisD"
                     /locus_tag="KPH29_001443"
     CDS             5012..6277
                     /gene="hisD"
                     /locus_tag="KPH29_001443"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBG1467062.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(6480..7232)
                     /locus_tag="KPH29_001444"
     CDS             complement(6480..7232)
                     /locus_tag="KPH29_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG1467063.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYVE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCHGKDWEN
                     VVNLDTKALLQCANKKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIHIFSQGGHPAILTNADEFAQLAKVFFKY"
     gene            complement(7350..7619)
                     /locus_tag="KPH29_001445"
     CDS             complement(7350..7619)
                     /locus_tag="KPH29_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467064.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            8094..9188
                     /locus_tag="KPH29_001446"
     CDS             8094..9188
                     /locus_tag="KPH29_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBG1467065.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISSITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVES
                     LRERSINGVISMCLKETDYQYSLSNKKGGY"
     gene            9190..10140
                     /locus_tag="KPH29_001447"
     CDS             9190..10140
                     /locus_tag="KPH29_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBG1467066.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGLKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINNNVEIANLASLVIDCAE
                     QGDKLAIEILRESSTELSKLAQVILRKISNPMKIDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRVKHG"
     gene            10133..11317
                     /locus_tag="KPH29_001448"
     CDS             10133..11317
                     /locus_tag="KPH29_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467067.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYEKNNIDKKSLMREMNNVSKGVIDNEDTPSGITEYINGKYKFKNKIWEDFFETKNQ
                     NIYKSLLEISSYFKSKGLKIGIDTFSPFISYFTGQDLKKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIHKNKEIGKRNFLDILGIKEYEGDVYPIEFVLEEIDFMTRNLNK
                     DIYVGVEINKKEIIAPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            11330..12826
                     /locus_tag="KPH29_001449"
     CDS             11330..12826
                     /locus_tag="KPH29_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBG1467068.1"
                     /translation="MSKILEMQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNSHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEEYMKICEDFDFNIDPHTVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVSD
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSEGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            12849..13799
                     /locus_tag="KPH29_001450"
     CDS             12849..13799
                     /locus_tag="KPH29_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1467069.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMLLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLVSFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            13820..14782
                     /locus_tag="KPH29_001451"
     CDS             13820..14782
                     /locus_tag="KPH29_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBG1467070.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            15154..15786
                     /locus_tag="KPH29_001452"
     CDS             15154..15786
                     /locus_tag="KPH29_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG1467071.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            16197..16289
                     /gene="kdpF"
                     /locus_tag="KPH29_001453"
     CDS             16197..16289
                     /gene="kdpF"
                     /locus_tag="KPH29_001453"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBG1467072.1"
                     /translation="MEGLPMWFLAVVIIMLIIYLVYALINPEKF"
     gene            16330..18012
                     /gene="kdpA"
                     /locus_tag="KPH29_001454"
     CDS             16330..18012
                     /gene="kdpA"
                     /locus_tag="KPH29_001454"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBG1467073.1"
                     /translation="MFMVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDN
                     FIYKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGL
                     SFNTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKK
                     KTLGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGP
                     VAALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKN
                     KKQGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSS
                     LFTTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVF
                     LCGLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGF
                     HGLSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGIVMFFGRYVSIIVLLAISSLLA
                     SKKAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            18037..20094
                     /gene="kdpB"
                     /locus_tag="KPH29_001455"
     CDS             18037..20094
                     /gene="kdpB"
                     /locus_tag="KPH29_001455"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HBG1467074.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNVGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMIL
                     NLG"
     gene            20113..20739
                     /locus_tag="KPH29_001456"
     CDS             20113..20739
                     /locus_tag="KPH29_001456"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBG1467075.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNASYGGVSSGSFNY
                     GATNPELHDRVQKDIEKFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVDDNTSKKLFGVLGEDRVNVLKVNIEVAKILGLI"
     gene            20976..21884
                     /gene="cysK"
                     /locus_tag="KPH29_001457"
     CDS             20976..21884
                     /gene="cysK"
                     /locus_tag="KPH29_001457"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBG1467076.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            21911..22501
                     /gene="cysE"
                     /locus_tag="KPH29_001458"
     CDS             21911..22501
                     /gene="cysE"
                     /locus_tag="KPH29_001458"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBG1467077.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
     gene            22661..22849
                     /locus_tag="KPH29_001459"
     CDS             22661..22849
                     /locus_tag="KPH29_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBG1467078.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(22903..23772)
                     /locus_tag="KPH29_001460"
     CDS             complement(22903..23772)
                     /locus_tag="KPH29_001460"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HBG1467079.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDMNKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDAINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(23969..25213)
                     /locus_tag="KPH29_001461"
     CDS             complement(23969..25213)
                     /locus_tag="KPH29_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467080.1"
                     /translation="MENNLNIAKKNNTELLQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYDMIKEINILIESITDKNELLSEELFTNNLSEF
                     YDESLDEIYIDLKQAYLYIKKNTDSLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNILDLEDIHSYYMENNTNITIYENNTLQYKFDEESEDIKDSSI
                     DIIEQMLILPDDLNYDKDEVENMKSRLAKINIKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
     gene            25504..25815
                     /locus_tag="KPH29_001462"
     CDS             25504..25815
                     /locus_tag="KPH29_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBG1467081.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFE
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKEIEQL
                     T"
     regulatory      25908..26000
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            26100..26894
                     /gene="thiD"
                     /locus_tag="KPH29_001463"
     CDS             26100..26894
                     /gene="thiD"
                     /locus_tag="KPH29_001463"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBG1467082.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            26918..27706
                     /gene="thiM"
                     /locus_tag="KPH29_001464"
     CDS             26918..27706
                     /gene="thiM"
                     /locus_tag="KPH29_001464"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBG1467083.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGIYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDTLSKNSNIEFLN"
     gene            27729..28361
                     /gene="thiE"
                     /locus_tag="KPH29_001465"
     CDS             27729..28361
                     /gene="thiE"
                     /locus_tag="KPH29_001465"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBG1467084.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITERYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEATCAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            28500..28802
                     /locus_tag="KPH29_001466"
     CDS             28500..28802
                     /locus_tag="KPH29_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG1467085.1"
                     /translation="MIKILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRVPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(28868..29617)
                     /locus_tag="KPH29_001467"
     CDS             complement(28868..29617)
                     /locus_tag="KPH29_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1467086.1"
                     /translation="MLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMNNI
                     NINNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQLLE
                     AILVSFIEVTILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLPNE
                     IVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETILI
                     GDILPVILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(29611..30573)
                     /locus_tag="KPH29_001468"
     CDS             complement(29611..30573)
                     /locus_tag="KPH29_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1467087.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNGLSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVKMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDSIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            complement(30577..31035)
                     /locus_tag="KPH29_001469"
     CDS             complement(30577..31035)
                     /locus_tag="KPH29_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG1467088.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNLPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            31391..31747
                     /locus_tag="KPH29_001470"
     CDS             31391..31747
                     /locus_tag="KPH29_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG1467089.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            31819..32535
                     /locus_tag="KPH29_001471"
     CDS             31819..32535
                     /locus_tag="KPH29_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1467090.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            32528..>33222
                     /locus_tag="KPH29_001472"
     CDS             32528..>33222
                     /locus_tag="KPH29_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1467091.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIFYGLGILSNLGFIQNILFFGMLGLFIYYIFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIVLITMIYGFLISQTATLFICKYIFNFIFRNIPIINMNFAK
                     DLYYLNLKSIPIDFISFMAIYVFLLLIAISIVNCCILFIFSYINKFYVILITLLIIAG
                     LYFSTIYINIINL"
BASE COUNT        12822 a         3615 c         5820 g        10965 t
ORIGIN      
        1 catctttgaa aagatgacta ttgataagta aggtataaaa ttggtaagta tcaaaaaata
       61 tactaaataa aaattatagt cctaatgagt taatttctgt attttcaaat aatgcagtat
      121 ttagtcatgt gtttttatat gcgcctgtat ttccaattac caatatatta gaataagcta
      181 ctgtaattag atttagaaaa aagcgacata aataattata aacaaataat aaattacata
      241 aaattaaatt tattcacaag tatatcttat aaaatatgtt ataattaata gaaaattgtc
      301 atataattaa ttaagtagta ttattataaa taacattgaa aggtagtggt tagaatatgg
      361 atatccataa taaatatgta aattttataa aaaatatacc tgtacctttt ttatattgta
      421 gaattgtaaa gagacaagaa gatatagaat atcgagtaga atatataagt aagggtatgg
      481 gcaaagtatt acaattagaa gaaggtattt gtgataaaaa tatactagat gtattgcctg
      541 tatttaagtc taaaaaatat ttcaaagagt tgttctcaaa tgaagtggat tgtataaaaa
      601 ggtacattcc gacacttaaa aactggataa atataaaaaa acagataata ggggattcat
      661 acataatttt gtattttggt aaaattgtat ttgattatag acagataatc gattcctttg
      721 ataaaaagga aaaagtagct tatattaaag acgaagaagg catatatata gattgcagtg
      781 aaaatttaat accaatatta aataacaaca ttaaaacaac gaaagatatc tttggtaaaa
      841 atgatataga agtatgggga gagaatacag gaaaattatt tagagatgat tatagaggag
      901 gagtatctag taaaaaaaga tttttacaaa atctttttga atatgaagaa acatttttta
      961 tggttgaaaa atattttttg tatgatgaag acgaactttt aggtactata gggataatag
     1021 ataatataat atatagtggt tacagtaata gaaattataa ctcaaaagat ttaatgaaga
     1081 tgatagaaca ctcaattcca gagaatatgt tttataaaga tgtgtatgga aattatattg
     1141 gatttaattc aggttttttg aatttagctt gtatgaataa agaagaatta ttagggaaga
     1201 atagttataa aatatcagaa gaagaagctt taataaataa gatatttgaa agtgataagg
     1261 gtgtagttga aaataaaaaa gtagttacat ttgagttaaa tgtaagtata aatgatgaaa
     1321 ataagtgtat tgagattaca aaaaggccgt tttttgatag ctatggaagt gttattggaa
     1381 taattggaac agcaagggat ataagtagaa gaaaaagatt agaagaggaa atggataaaa
     1441 ctaggatgga attttttgca aatttatccc atgaacttag aacgcctata aatttaatat
     1501 catcttcatt acaagtgata gaaaaaaaag aagcagattt aatagaatca aatgatactt
     1561 taaaaagaaa tttaggtata ataaaacaaa atggaaatag aatactgagg ttagtaaata
     1621 atgttataga ctttacaaaa atgcaatcag gatatttaga ttttaaacca gaagaatctg
     1681 atataattgc atttatagag gagatatgta tgtctgtagc tgattttgca agccaaaata
     1741 atatacaact tacatttgat acagaaatag aagaattttc aatgttattt gattcagaaa
     1801 aattggaaag aataatatta aatttacttt ctaatggaat taagtacaat aagaaagatg
     1861 gaaaaataaa tatattttta tatgtgaaag ataatgtatt taatatgaaa atatctgata
     1921 gtggaatagg tataccaaaa gaaaaaatag ataaaatatt taatagattt gaacagattg
     1981 acaatgaact ttcctacaga gttaaaggaa gtggtattgg actttcatta gtgaaatctt
     2041 tggttgaatt gcatgaggga agtatatctt taaaaagtca acttggtatt ggaagtgaat
     2101 ttatagtttc attacctgtt agaagtgaaa ataatattga aaaatataat cataaaagag
     2161 aaatcagcaa tgaactaagt aaaaaattag aaatagagtt ttcggattta taagtttaaa
     2221 tataaaaaat aaaattttaa aaaagtattg acaattgatt tagaagggtt taaaataaat
     2281 tacaacataa taaataaaaa ggcgatgaat aggaagagta aatctaattc gattaccaga
     2341 gagaagagtc cattggctgg aagacttttt aaattagagg gattgaaaac tacctaggag
     2401 ctactttctg aagtcatctg cattagtaga ttttagtaag aatagtcgtt agctgcgtta
     2461 aagtgtaaag tgagttttca tgatatatga aaactaaatt aggtggaacc gcgggaataa
     2521 tactctcgtc cttatgttat aaggatgaga gttttttgtt gtttaaaaat ataaacagta
     2581 atcataataa ttatagaaaa tataaaagcg atgaatagga agagtaaatt taattcgact
     2641 atcagagagg aaagttcaca ggctgaaaga ctttctaagt ttgagaaatt gaaaactacc
     2701 taggagctgt ggtctgaagt tactttaaaa agttttagta agactaaccg ttaactgcgt
     2761 taaagtgtaa agtggatttt atcaataaat gataaaatca aattgggtgg aaccgcgagt
     2821 atagactcgt ccctttatat agggatgagt ctatttttat tgcaataaaa gattgaaaat
     2881 aaaaaaaaat cggaaaatta taaatttagg aggaaaataa aatggaaaat aaagtgaaaa
     2941 ttggagtact aggatatgga gttgtaggaa gtggacttat agatataata gataataata
     3001 aagaaaaaag aagtatagaa atagttggaa ttttagttaa taatttggaa aaacataagg
     3061 ataaaaaata ttctaatata ataactaata atatagatga tatatttaat aaagatatag
     3121 atatacttgt tgaagtcatg ggtggtttag aaccatcatt atcttatata aaaaaagcat
     3181 taaacaataa aattcatgtt gttacagcaa ataaagattt gttagctgaa tgtggagatg
     3241 agcttgcaaa attagcaagt gaaaataaag taagtattaa atttgaagct tcagtcgcag
     3301 gaggtattcc agtattaaaa ccaataatag aatcactaga aggaaataat atagatagta
     3361 taaatgcaat tttaaatgga actactaatt ttatattatc taaaatgtat gatgagaatc
     3421 tatcttatga tatggcatta agacaagctc aagaattagg ttttgctgaa gcaaatccag
     3481 agtcagatgt attagggtat gatgctgcta gaaaactatc aatattatca actctagctt
     3541 atgacaatag agtatattgg aaggatgtgt atttagaagg aattacagat atagatgaaa
     3601 aagatattga atatgcaaaa aaattaaatt gtaaaataaa attgattggt caaagtaagt
     3661 atgaaaatga taaagtaagt gcttttgtta gacctgtttt agtagaaaaa gataatatcc
     3721 ttgctagaat agacaatgag ttcaatgctg tcatagtgaa tggagattca gtaggagaag
     3781 tttcttttgt aggtaaagga gctggaagtt tagcaacagg aagtgcggtt tattcagatg
     3841 ttatagatat aatagataat agagtttcaa gtatagattc ttttactaaa gataaaatac
     3901 aagttaataa aatagttaga gaaaagtgtg gagcacttct tagatttaaa aaatgtaata
     3961 aagatgaaat tctaaatata gttgagaatt gtttagtaaa attcgatatt ttaaatgatg
     4021 atgatgaact tgctattatg gtttatgctg attctgaata tgaaataaac aacagtttat
     4081 gtctaataaa agataaagga tattgcgaaa aaatgaataa aatgttgaaa ataagttaaa
     4141 aaagaaaaaa taagtttata tgtaacatat tttgacgatt ttaatactat atagtatgag
     4201 atgaaacaaa acatctcata aaaaattaaa attcaaggga gagatatgtt atatggaaaa
     4261 taaaaaatat gcaaatggtg gttattcaga agactggtat gaaagaggag aatctacagc
     4321 taaatggttc cagaatgata gagaagaata tgaaagagca gcatacgatg aagatagaga
     4381 aagaagaggc tcaaactgtg gatgttcaga ttcaggagga aatagaccta gaaactgtga
     4441 aagatttaga agagaagctg agataagaga aagagaagca agagaagcat tctgtgaatc
     4501 ttcagagaga aagaaagaag cactagcata tgaatgtgaa gctagaaaat tatgggaaga
     4561 agcagaaaaa tactgggatg aatattcaaa atacaactat aaaggaatcg aatatttagc
     4621 agaagctgct agattatttg atgaaggtat ggaatgtgaa gctagaagaa atggaaataa
     4681 tggaggaaac aataataatt gttgccataa atgccataaa tgtaattgta actgctgtag
     4741 aaaataaaaa aattcattga caaatcaata gttaaaatat atcatattat gtataataaa
     4801 gagaaaattc agaaaaaaat aatgattaga ataaatttaa gtatatttat tagacaaata
     4861 gtgctcagtt agtgaggtga gtacaatatg attaaactcc catcctctag cttttgatag
     4921 agggtgggag tttttattta ttgatatatc ttaaatgagg ataatattgt aaaataaatg
     4981 agtatgagat tgtaaaatta ggaggtaaaa aatgaaaagt attaaaacat caagtatgaa
     5041 agaaaaggta gatgaaaaga tattaagaga gcaagtaagt tctataatag aagatataag
     5101 aaataataag gatgttgcac ttaaaaaata taatgagaag tttgatagaa atacaagaga
     5161 tgaatttaga attactaagg aagaaataaa ggaagcttat aagcatgtag atgacgaatt
     5221 tataaataac ttaaagattg ctgccaaaaa tataagagaa tttgctaaag ctcaaaaact
     5281 aagttttgaa aacccttttg aaaaagaaat ttatccaggt gtaatcttag gtcaaactaa
     5341 tattccaata gagtcatgtc ttgcatatgt tccaggaggt cagtatccat tgttttcaac
     5401 agctcttatg cttataatac ctgcaaaagt agcaggagta aaaagaatag tggcatgttc
     5461 accaactatg aaaaatactg aaaagataaa tcctaaaaca ttggtagcta tggatatagc
     5521 aggtgctgat gaaatatatg caactggagg agttcaagct atagcagcat ttacatatgg
     5581 aacagaaaag attaatccag ttgacataat tgtaggacca ggaaataagt ttgtaactga
     5641 agctaaaaga caatgttatg gacaagtagg aattgatttt gtcgctgggc caagtgaagt
     5701 tcttataata gcggatgaaa cttcaaatcc agtttatatt gcagctgatt tattagcaca
     5761 atgtgagcat gatttaaatg caagaggaat attattgaca aatagcttgg aagtagcaca
     5821 aaaagttgaa aaaaatatag aaagtatgct aaaagatttg ccaacaaagg acatagcata
     5881 tagttcatgg gaaaataatg gagagataat attggttgat gatatggaag aagccattaa
     5941 aataagtaat ttttatgcac cagaacattt agaagttgca gtaaatgagt ataatgatat
     6001 ttgtgataga ttaactaact atggttcttt atttattgga aatctttctg ctgaggtgtt
     6061 tggtgactat gtttcaggta ccaatcatac attaccaaca cttaaagcat caagatatac
     6121 tggtggtgtt tgggttggaa cttttatcaa aacttgtaca aagcagatat ttaatgaaaa
     6181 agctatacaa tcattagcac ctgtagctga aaagctggca aaagaagagg gattatatgc
     6241 acatgcaaag gctgctgagg ttaggtttaa aaagtagtaa aaaattttat tttggcagtc
     6301 gagatttagt attagactga gataaagaaa acgctgagaa cctgaataaa tactatattt
     6361 tgtgggcctc ggcgtttctt ttatatctgt atataatagg ataatactac ctgtgtgttt
     6421 aaatgctaac ttaatatttc ttttctaaat aatgtttatg aaatagttaa tatttttagt
     6481 taatacttga aaaaaacttt tgctaactgt gcaaattcat ctgcatttgt caatatagca
     6541 gggtgtcctc cctgactaaa aatgtgaata cttgcttttg aaataaaagt tgccatttgc
     6601 ttgtactctt cttcaagatt atgacgacac atttcgtctt ccttacttcc tgtaaataaa
     6661 attggcattt ctaatttaca caattcttta tgaaataaag ggcgtttctt gtttgcacat
     6721 tgcaatagag cttttgtatc aagattaaca acattctccc aatcttttcc atgacaccat
     6781 tcatagaatt ttcgtgcttg aatatcttgc tttgattttt cccttccagt gattagatta
     6841 tctatgaagt tatcattaag agtacgtcca tcaaagctat ctgctataat tgcttcaact
     6901 aaatcgggac gttctaatcc agtattaaca gctgcccatg ctcctccact tgttccaatt
     6961 aagccaactt taccacaatt caaatgttca atcagtgcta ttgtctgtaa ggcttcatca
     7021 tgccatatgt caggagaaaa ttgagaaact ctatcagact gtccattacc taaaaaatca
     7081 attaagatac atctaaaatt ttctacatat aatggcacaa gtgctttaaa catattagaa
     7141 gaaccagtat taccatgcag gaaaattatc ggtttacctt gtccatattc tttataaaaa
     7201 caagaattac cttgatactg aaaatatgac atatattttc ctccttttta tttgttaagt
     7261 taattataac aatataggta tgaatgaaaa acctcatgtt ctttcttaga ataaaaatgt
     7321 gccacgcata aaacgtgaca catttctaat tataaatttt gctgtttaag tattctttgt
     7381 atgcttttct ctgtcaaaaa gtatttctct gataactgct tgatactcat tccattttgg
     7441 aaatttatac agatttgaga attacgagaa gctaagactt gtttggtgtt tgtgttttca
     7501 ccccaatgct ttttgtgctc ttgctttttg ggaatataaa tcactttacc atctatatat
     7561 tcttgcactt gctcaatcag ttctttaggc agtaagtcaa tcgaattttg atagctcatt
     7621 ttgcacctcc taaataatta ctctttagga ttagcaaagg ctactgctac tcacaattaa
     7681 aaataaaatt ttttattatg tacaatagcc tttgctatgc acaagttcca attcccataa
     7741 actaaatact cctttctggt ttgaaaaatt catctgaaca ttgatttcga ttttaagata
     7801 gagttcaatg acaaatttac atttttatta tatatattca gatagataat tgcaactgtc
     7861 tttgtatgag gatattttat tatagtcaat actataatat atatttatat catcaaacta
     7921 atatgaacag ttgaaaggaa tatgaacaat aaaacttagt caggaagaat aattatgaag
     7981 cagacaattg agaaatttga ttttaaagtt ttattgaaaa tttattgaca aatttaactt
     8041 gtaaatatta tactaaactt aataaataaa gtttataaag taaggtgttg tctatgaaaa
     8101 atatagttgg aaaaccgcaa acgataaaaa aagtaaatga agatttaata aaaaacatta
     8161 taaaaaatga aggtcctata acaaaaccag aaatttcaag cattactgaa ttgagtttag
     8221 ctacagttaa taagattgtg gagcaattag ctttgaaaaa tgaagttaaa gtaagtggac
     8281 taagtgaatc tactggagga agaagagctc aattatttga aataaatgca aacttagaac
     8341 acataatagc tttatattat tatagaaatt gttgtatagg ggtagttgcc aatttattag
     8401 gtgaaataat ttatcaagaa gaatttaata taagaatgga tacatatgaa aacgttaacc
     8461 aagatacttt ttttgttata gatactctaa taaaaaagga aaaaggaaac aatataaggg
     8521 ctattggaat tggtgtccca ggagttgtaa aaaaaggtgt agttaaaaat ataccaacta
     8581 taaaaagttg ggaaggtata aatttagaaa atttaataaa gagtaagtac aatactaaag
     8641 tgtttataga aaatgataca aatattacta ctattgggat ttatcaaaaa cagtataaag
     8701 acaaatataa aaatatggct cttatgtatt tagaagatgg tattggttca ggtatagtta
     8761 taaatggtga attgtattta ggttctacaa attttgctgg agaattaagt tatctaaata
     8821 tagaatttgg taagtataac tctaacaact taaatcttga agattatatt atgagtttaa
     8881 gaaaaagata tttagaaagt aaagataata aatttaagaa agaaatttta tctataatat
     8941 caaactttat aaggactctt gtatgtgtcc taaatccaca agctatgatt atacagtgta
     9001 gtatattaac caagagtgac atggagttta tagaaaaaga tgtaagtaat aatgttggag
     9061 aagaaaattt tcctaaatta ataaaagtag agtcattgag agaaagaagt ataaatggag
     9121 taattagtat gtgtttaaaa gaaactgact atcaatattc cttatctaat aaaaaaggag
     9181 gatattaatg tgaaatattt ggtttcaata gatggagggg gaacaaaaac taaattttgt
     9241 gtaagtgact tagatggaaa catacttaaa gaacatacca ctggttcaac taattataag
     9301 tcagttggaa ttaaaaaaac atatgaaaat attaataatg gacttaaaaa aatattaaaa
     9361 gatttatata tcgattatga cgatattgag tacactgtat ttggtatatc tggttgtgat
     9421 tctcctaatg attataagat tataatggat gaaatattaa aaattgggat taacaaagaa
     9481 aaaatatatc tggcaaatga tgcagtttta gctttttatg cacaagctga ttcccctggt
     9541 ttagtaattg tggctgggac aggttcaatt atactgggaa ttaaagaaga tggagaaata
     9601 tatagagttg gtggatgggg gtataatttt agtgatttgg gttctggata tgatataggg
     9661 agaaaattat taaagaaagt attgctttat tgtgatgaat gtcatgagta tagtgattta
     9721 tttagttgtg tcttggattt ctttggagca aatagttttg aacaacttgc atatatgatt
     9781 actgatataa acaataatgt tgaaatagca aacctagctt cattagtgat tgattgtgcc
     9841 gaacaaggtg ataaattagc tattgaaata ttaagagaat catcaactga actatctaaa
     9901 ttagctcaag taatattgag aaaaatatct aatccaatga aaatagataa aagcgaaata
     9961 aatatagttt tgtctggtgg tacattaaat aaaactgtat atgctcaaat gttaatgaat
    10021 aatttaaaaa cagaaaatac agataaaaat ttaaaattta tatctcaaga aaatcagcca
    10081 gtatatgggg ggataagact tgctatggca ttagctaata ggagagttaa gcatggatag
    10141 aaaaaaggta atagtacaaa tttttactgg aggttttaag gctaaagaaa taaagtttaa
    10201 tgatataaaa tgtaaactac tagaattatt ggactgtatt gatatagaaa aggttatcat
    10261 gggatggagt gtagatgaag aattatatgt agaaactaaa aaactattaa ataaatataa
    10321 tgttgaacta tatctttggt tgccatgttt ttctgaaata ggattacttg aaaaaagtaa
    10381 tttactaatt gattatatgg ggaaggaagt caagggctat gctttacaag aggatgaaaa
    10441 ttttgaattt tattgcccaa ataataagga aaatataaaa aatataaaga aaatatatag
    10501 tacttatttt agtgatatta aatttgatgg aattttttta gataaaataa ggtatggttc
    10561 tttttcgaat aaactaaatg gtgtttttaa ttgtttttgt aaagattgtt taaatatata
    10621 tgaaaaaaat aatattgata aaaagtcgtt aatgagagaa atgaataatg tgtctaaggg
    10681 agttatagat aatgaagata ctccatctgg tataacagaa tacattaatg gaaaatacaa
    10741 atttaaaaat aaaatatggg aagatttttt tgaaacaaaa aatcaaaata tatacaagtc
    10801 attattagag ataagtagtt attttaaaag taagggattg aaaataggta tagatacttt
    10861 ttctcctttt atttcttact ttacaggtca agatttaaaa aaattacaag aagtagcaga
    10921 ttttataaaa ccaatgatgt atagaactac aaaagctcca gctggtttac catttgaatt
    10981 tgaaaggttt attgatgaat ccatacataa gaataaagag attggaaaaa gaaatttctt
    11041 agatatttta ggaattaaag agtatgaagg agatgtatat cctatagaat ttgtactaga
    11101 ggaaatcgat tttatgacaa ggaatcttaa taaagatata tatgtaggag ttgaaataaa
    11161 taaaaaggaa attatagctc ctgttacacc taagtacata agtgaaaata tttcaaatat
    11221 aaataagaca acagtaaaag gatatgtttt atcttgggat ttgttatcag caccaaaaga
    11281 aaatattgat gaaattataa aatggtttcg gaattagtga ggtgatatag tgagtaagat
    11341 attagaaatg caacaaatat ataaaagttt tggaaaggta gatgttttaa aagggataaa
    11401 ttttgagtta gaaaagggag aagtaagagc tcttttggga gctaatggag caggaaaatc
    11461 tacacttatt aaaattttag gaggagttca ttctcaaact aaaggtaaag taattttgac
    11521 tggtaatgaa ataatgttta aaaattcaca tcattctaaa gaaaatggta ttagtataat
    11581 ttatcaagaa ttaagcttga taccaacgct tacagtactt gaaaatattt ttcttggtag
    11641 agaaatttta aaaggaagtg tgtttttaaa caagaaagac atgcttgagg aatatatgaa
    11701 aatttgtgag gattttgatt ttaatataga cccacataca gtagtttcta atttaagtgt
    11761 agcaaaccaa caaatggttg agataatgaa agctgtttca tgtaattctg aaattatcat
    11821 aatggatgaa cctacaacat cacttactaa taatgaaaaa gatgggctat ttaaaataat
    11881 aagtagacta aagtctaaag gtaaaacaat agtctatatt tctcacatat tagaggaagt
    11941 ttttaaagta tgtgatagtg caagtataat gaaaaatgga attatgattg gaacttatga
    12001 tgtagaggat ttaactaaat taaagataac tcaacttatg acaggaaatg ataatcacaa
    12061 acttattgag aagaaggatt atacatttgc aaagtatgaa gagccaccag tactagaagt
    12121 aaagaatata agctggtcta acaaaataaa aaatgtttca tttaaagttt atagaggcga
    12181 agttgtaggt cttgctggtc ttgtaggttc aagaagaact gaaattataa atttgatatt
    12241 tggtatagat aataaagata gtggagacat ttttatcaat ggtaaaaaag tagacataac
    12301 ttctcctaag gttgctataa aaaacaaaat aggattgatt cctgaagata gaaaaaatct
    12361 aggattaatt ttagaacaag ctatatacac aaactcatca tcagtacaaa ttgataaatt
    12421 caagaaattt ttgtttatag atagaaataa agaaattgag ttttctaaaa aaggtgttaa
    12481 ggaattagga ataaaagtta gtgatgtaaa gcaagaagta aaagagctta gtggtggaaa
    12541 tcaacaaaaa gtagtagttt ctaagtggtt agagaaagat ttagatttaa taatatatga
    12601 tgagccgaca aagggaatag atattgctgc aaaagaagat attttcaata caatcaaaga
    12661 attttcaagt gaaggaatag gagtaatatt tatatcttct gatttagaag aagtaataag
    12721 agtaagtgat agaattttag taactagaga aggttctatt gtagatgatt taaaaaatga
    12781 gaatatacag gttcaagata taatgaacag tatatttaat gtataaaaaa tgaaaatgga
    12841 ggactgagat gaagaaagct tttgagaagt taaatttaag agattatgga gtattattag
    12901 gttttttagc tttatgtata gctataagta ttgcaactcc atcattttta ggtaaacaaa
    12961 atatacttaa tcttttacga caatcttcta taattggaat aatttcagca ggtatgactt
    13021 ttgttataat atctggaaac tttgatatat cagttggagc agtagcagct cttgcaggtg
    13081 ctataacaat gaaatttgcg actggtggaa ctaatttgtt tgcatccatg ctcttaggtg
    13141 tagcgatttg tgcagtaata ggattaatca atggtatagt agttgcaaag ataaatgtac
    13201 catcattaat agcaactatg gctatggtca ctattgtaag aggattgctg ttgatgctta
    13261 caggaggata tccaattact gaaaatatac caatacttga ttacattgga aatggatatt
    13321 tgtttaatat accaatacca gtagtaattt tctttatagt ggtgttagtt tcatttattg
    13381 tacttaataa aactaagttt ggtagatatg tttattctgt aggagggaat caagaagcaa
    13441 gtaaacttaa tggtataaat gtagattctc acaaagtgaa agtgtttatt attaatgctg
    13501 tacttgcagg aatagctgga atcgtactaa caggaagact tgggactgca actgctattg
    13561 caggtgaagg atatgatatg gatgcaattg catctgtagt aataggaggt acatcagtgg
    13621 caggaggttc tggttctgta cttaaaactg ttataggagt attacttatg agtgttataa
    13681 acaacagttt taatcttctt ggaatagatg tatatttcca gtacatattt aaaggattaa
    13741 taattttagc agcagttgga tttgattctt atagcaagaa aaaacttgct tcaagataaa
    13801 aaataagggg gattttttta tgaaaaaatt aatgaaacat ttagcattgt tgttatcact
    13861 tgttatgata tttggtttag ttggttgttc aaatggtggt gacaaagaaa aaaatggaga
    13921 aaagaaaata gcagtattac taccaggttc aacaggttac tttgtagcaa ctaaacaagg
    13981 tatagatgca aaatctaaag aattaggtgt aagcgtggaa tatgcagatg ctcaatggga
    14041 tgctagtaaa caattatcac aagctgaaga ttttatggct aaaggggtag acatgattgc
    14101 attatgtgga gttgattctg ctgtaagtga gagaatagtt aaagctgcta atgattcaga
    14161 cgtccctata gtagcattta caaattctat aggaagtaac ccaactggag aatttaaagg
    14221 tttagttaca tatataggac aaaatgagga ggaaacagga gcactaactg gaaaaattgc
    14281 taaaaactta ttaggagaaa ctggtgggaa agctgtatta atagaaggag taccaggaac
    14341 tactccacaa gtattaagaa aaaaaggtct tgaaaaagaa ttaaaagata gtaatataga
    14401 aatagtttat aatcaaactt caaattggga gaaagagcaa gctttaaaag taacagaaga
    14461 tttaattcaa aagaaaactg attttaatgt tataatatgt caagatgata attctgctac
    14521 aggtgcaggt caagctttaa aagatgctgg tcttaaggat aaagttaaag tcataggttt
    14581 gggtggaagt aaagatggat taaaagctat aactgatggg ttaatagatg gtacaactta
    14641 tatgtctgct gtagaagaag gtggattagt catagaaaag gcatctaaat tccttaaagg
    14701 tgaaaaaata gaacctgtta cacaaattaa acaagttgaa gttaacaaag ataatatttc
    14761 agagttcaag ggtgaatggt aaattgtcat agataataaa taagattttt agagttactt
    14821 tagaaactat ataaagtaac tcttttttta atttaattta gtgataaatt attgaatagt
    14881 atttctaaac aaataaaaaa ttaatataaa caaagactta attatactaa attataagta
    14941 aaactaatat aaacttatgg aaaaaaggaa aacgtctaaa gtattggaat tactatagtg
    15001 taggtgtggt attaaaaaaa cataaaaaaa cataaaaaaa ttgttgaaaa aaatatagaa
    15061 attttgtata attaaagatt tgaaaagttc ggtatacaaa gccaattctt ttgtatataa
    15121 tatatataaa ttcagaaaaa ggaagggata tgtatggcca ggcggttatt tttagaaagg
    15181 attggactta tatttttagg ttcagcaata ttagcatttg gagtttataa tttttattat
    15241 ttaaacaaca taacagaagg tggagtgtta ggtattttac tactgttaaa aaatttattc
    15301 aatatacaac cagcaatagc aaatgttgta atagatggtt tactactttt agtaggatat
    15361 aaatttttcg gtaaaaaatt ccttatatat tcaatagtag cttctataac tttttcagtg
    15421 ttatatgact tatttgaagc tataggacct ctagtaccac aatcacaaaa tatgctttta
    15481 tcaactattt tggcaggtgt aactgttgga actggtgtag gtatagtagt aaaagcaggt
    15541 tgtgcatcag gaggagacga tgctttagca ttagtaattt ctaagactac atctttgaac
    15601 atagggcaag tttatttagc aacagatgtt attgtattgt tgttatcatt attttatcta
    15661 tcagcgtttg atatctttta ttctttgatt gctgttacaa ttagtggcaa ggtaattgat
    15721 tttatttatt atcatggtaa aagtttggat atggatataa gtaatgatat agttccagaa
    15781 tgttaatata aattaaataa ctttagattt tattaggggt ggcagatttg ccaccttttt
    15841 tgtgtctaaa tctttataga aagtataatc ccatctaggt ttttgcaaat ctcaaatata
    15901 gaattatgtt ggaatgactg ggaatacgat tgctagatgt gcattttaaa aacccaattt
    15961 tatatttatg aaatactaat cattaatgga tgtttcttaa tcctatcttt atacctaaaa
    16021 tgataagata aatgaagtag caatacaaaa ctaaaatttg taaaagagat taaattaaat
    16081 ggaggagcta aaatgttgga tatagttatg atagcattgt tattcatagg atttgtttcc
    16141 atgaagttac ttgctgattg gtgtgggaaa caaactgaga aaaaataatg taatgtatgg
    16201 agggattacc tatgtggttt ctggcagttg taattataat gttaataata tatttggtat
    16261 atgcgcttat taatcctgaa aaattttaaa cagtataagg ctaataagta agttgaattg
    16321 gggggaacaa tttttatggt gcagatagta atagtgttag cgatttttat gattttagta
    16381 atacctatgg gcaagtattt atatcatata gctactaatc aaaaaacatt tggagataga
    16441 ctatttgata aagtggacaa ttttatatat aaggtatgta gtatagataa gaaaaaagaa
    16501 atgaactgga aacagtatgc attagcttta ttatttacaa atgcagttat ggtatttata
    16561 ggatatatca tacttagaac tcaaagtatg catattttta atccaagtgg aattaaaagt
    16621 atggaacaag gactatcatt taatacaata ataagtttta tgacaaatac aaatcttcaa
    16681 cattattcag gtgaatcagg actttcatac tttagtcaga tgacagtgat aatatatatg
    16741 atgtttacat cagcagcaac aggatatgcg gctgctatgg catttgtaag aggtctagtc
    16801 ggtaaaaaga aaacattggg aaatttttat gtagatttaa ttaggataac aacaagagta
    16861 cttttaccag gagctttaat tattgggctt atattagtta cacaaggagt accacaaact
    16921 tttgctggga cagaaacagt gactactata gaaggaaagc ttcaagatat agctagaggt
    16981 cctgtggcag ctcttgaatc aataaaacat ttaggaacaa atggtggtgg attctttggt
    17041 tcaaattcat cacacccatt tgaaaatcca acaataatat caaatatagt agaaatatta
    17101 tctatgatga tattaccagg agcttgtgtt gttgcattcg gtcacatgat aaagaataaa
    17161 aaacaaggtt gggttgtgtt tggcgcaatg tcaatcatat ttttaatagg tctagtagtg
    17221 tgctttaaag cagaaagtgc aggaaaccct attttatcac aattagggct taatcaaagt
    17281 atgggaagta tggaaggtaa ggaagttaga tttggtatag cacaatcatc attattcaca
    17341 acagtgacta cttcctttac gacaggtaca gttaataata tgcatgatac actgactcct
    17401 ttaggaggat tagtaccact tttaaatatg atgctaaatg tagtatttgg tggtaaaggc
    17461 gtaggtctga tgaatatgtt gatgtacgct ataatagcag tattcttatg tggattaatg
    17521 gtaggaagaa caccagagtt tttaactaag aaaatcgaag gaaaagaaat gaagcttatt
    17581 gctttactta ttatattaca tccactttta atactaatgt tctctggtct ttcagtagca
    17641 ataccagcag ggcttgaagg aatttcaaat cctggattcc atggattatc acaggtattg
    17701 tatgagtttg catcttcagc agccaataat ggttcaggat ttgaaggtct aggagataac
    17761 actatgtttt ggaatataac aacaggtata gttatgttct ttggaagata tgtgtctatt
    17821 atagtactac tagcaatatc aagtttgtta gcatctaaaa aagctgtaaa tgaaagtata
    17881 ggaacactta gaacggataa ttttacattt acaatagttt tagtacttgt tgttttaatt
    17941 gttggtgctt taacattttt cccagcctta gcgcttggac ctatttcgga gcatctagtg
    18001 ttgtggcact aaaaaattgg gagggagtaa agttttatgg aaactaaaaa aacaaagttt
    18061 attacaaaag atattttcaa aacttccatt atagaagctt ttaaaaaatt aaatcctaaa
    18121 tatatgatga aaaatccagt tatgtttgtt gtagaaattg gatttatagt aacattgata
    18181 cttacaatag tacctagttt gtttggtgat gaaggcaata atttaagagc ttataatggg
    18241 attgtaacaa taatcttatt tataacagtc ctttttgcaa attttgctga agctgtagct
    18301 gagggaagag gtaaagctca agcagataca cttaagaaaa ctagaaaaga tacagtagca
    18361 agggttatag atacgttagg aaatgaaaaa atgataagtg caagtgagct aaaacttggt
    18421 gatattgttt tagtaaatgt tggagaagtt atcccaaatg atggagaagt tatagagggc
    18481 atagcttctg tggatgagtc agcaataact ggagaatctg cacctgtcat gagagaatca
    18541 ggtggagatt tcgcatctgt tacaggtggt actacagttg taagtgactg gttaaagata
    18601 agaataacaa caaaaccagg agaatcgttc ttagataaaa tgatagctct tgtagaaggt
    18661 gcttcaagac aaaaaacgcc aaatgagatt gctttaaata ctttgttagt agggttaaca
    18721 cttatattta tggttgtttt aatatctctt tatccaatgg caaactatgc aggtgtaaag
    18781 attccaattt ctactatgat agctcttttg gtatgtttaa taccaactac tataggtgga
    18841 ttgttgtcag ctattggtat tgcaggtatg gatagagtaa ctagatttaa tgtaattgca
    18901 atgtcaggaa aagcagtaga agcttgtgga gacgttgata ctatgatttt ggataagaca
    18961 ggtacgatta cttatgggaa tagacttgca tcagaattta tagcagttgg aaatgcagat
    19021 tcaaaagatt taataaatta ttctgttatg tgttcattaa aagatgatac accagaagga
    19081 aaatctatag ttgatttagg tatgaaatta ggttcaacag gtaagacaaa agaggcagag
    19141 gaagctgagt ttgtagaatt ttctgcacaa actagaatga gtggtattaa cttagctgat
    19201 ggtagagcta taagaaaagg tgcttatgat agcataataa aaagagtaaa agaatctggt
    19261 ggtacagtgc cagaagattt agaagaaaat gtaaatcgag tagctaaact aggaggaaca
    19321 cctttagtag tgtgtgtaga taatgagata tatggaatta tctacttaaa agatacagta
    19381 aaatcagggc ttgtagaaag atttgctaga cttcgagaga taggaataaa gacaataatg
    19441 tgtacagggg ataatccctt gacagcagct acaattgcaa gagaagctgg agtagatgga
    19501 tttattgcag aatgtaaacc agaagataaa atagatgcta taaaaaaaga acaattagag
    19561 ggtaaaatag ttgcaatgac aggtgatgga acaaatgatg ctcctgcact tgctcaggca
    19621 gatgtaggtc ttgcaatgaa tagtggtaca acatctgcaa aagaagcagc aaatatggtg
    19681 gatttagatt cagacccaac taagatattg gaagttgttg aaataggaaa acagctttta
    19741 ataactcgtg gggctttgac aacatttagt atagcaaatg atgttgcaaa atattttgcg
    19801 attataccgg ctatatttac tcttgcaata cctcaaatgg agatattaaa tgtaatgaaa
    19861 ctttctacat catttagtgc gatattatca gctttaatat ttaatgctat aattatccca
    19921 tgtttaatac ctcttgcaat gaagggagta aaatatagac ctatgaaatc acaagctatg
    19981 ttaatgagaa atatgcttat atttggtctt ggtggagtta tagtaccatt tttaggaata
    20041 aaattaattg atttattgat tacaccttta gtaatgattc ttaatttagg ttaataaatg
    20101 gaggatataa aaatgaaaac attaaagcca gcattacttg taagtattgt attgttagtt
    20161 gtttgtggac ttgtatatcc acttgttttg acaggtgtaa gccaagttgc atttaaagat
    20221 aaagccaatg gaagtatgat agaagtaaat ggtgtaaaag taggttcaga acttattggt
    20281 caaagcttta cagatgcgag attctttaag ggaagagtat catcagtaaa ctataatact
    20341 tatacaaaag aagatttagt accagataaa gatggaaatg catcttatgg aggtgtatca
    20401 tcagggtctt ttaattatgg agctacaaat ccagaacttc atgatagagt acaaaaagat
    20461 atagaaaagt ttttaaagga taatccaact gtcaaaagag aggatatacc aacagattta
    20521 cttacagcat caggttcagg acttgaccct aatataagtc cagcctctgc aaaaattcag
    20581 ataccagcta tagcaaaagc gtctggaata tcagaaagta aattacaaaa aattgtagat
    20641 gataatactt ccaaaaaact ttttggagtt ttaggtgaag atagagttaa tgtactaaag
    20701 gttaatatag aggtcgcaaa aatattaggt cttatatagt attttgtgga attttagtaa
    20761 caaaatatta ctcaagattt aatttaaagg ttaaatttta aagagtgaaa atcaataatg
    20821 attttcactt ttttatttga atattactaa tatttggaat aaagtgattg taagtataaa
    20881 atccaaaaaa aggttgcatt atcaaaatga taatactata atatatttat cgttatcaat
    20941 ttgataatag atttggacat ataggaggaa atagtatgtt atataataac gcattagagt
    21001 tgataggaaa cacacctgta gtaaggctga ataatttagg ctgtactaat gggtgttcta
    21061 acatatatgt aaaattagaa aaagtaaatc cagcaggaag tataaaagat agagctgttt
    21121 atgaaatgtt agagggctta gagcaaagag gagaattaaa aaagggagat gtactagttg
    21181 aagcaactag tggaaatact ggtattgcat tatctatgat aggaaaatta aaaggatatg
    21241 aagtagtaat cgtaatgccc gaaacaatga gcgttgagag aagagattta atgaaggctt
    21301 atggagcaaa tttggtgctt acagatggta aagctggaat ggcaggttcc atagaaaaag
    21361 caaatgaact tttaaaagaa aatccaaact ataaaagctt aaaacaattt gaaaataaag
    21421 ataatccaaa tgcacattat aaaacaacag cagttgaaat tatgaatgat gtcaaagatt
    21481 tagatatatt cgtatgtgga gttggaacag gtggtacttt aataggaact gcaagatatt
    21541 taaaagaaca aaatccaaac ataagagtaa ttgcacttga accaaaaaaa tctccagcta
    21601 tatcagaaaa caagactgga cctcataaaa tacaaggaat aggtgctaac tttgttcctg
    21661 aaaattatga ttctagtgta gttgatgaag taatattagt agatgatgaa gatgcatttg
    21721 atacagttaa attacttgca gcaaaagagg gaattttagt agggatatct tctggagcaa
    21781 atatatttgg tgctatagag atggcaaaaa agtatccaga taaaaagata gttacagtag
    21841 ctccagatgg agttgataag tatatgtcta tgggaatatt ttaataattt tatttgaggg
    21901 agtgaatact ttgtttaaga aaataaataa agatattgaa tatataatga agaatgatcc
    21961 agcagctagg tctaagatag aagttttttt actttatcca tcagttcatg caatgataat
    22021 gcataggatg gctcatgctc tttataagaa gaagaagctt tttacagcaa gattaatatc
    22081 ccagatatcc agatttatga ctggcataga aattcatcct ggtgctaaaa tgggggaagg
    22141 tatattgata gaccatggaa tgggtgttgt aattggagag acagctgaag taggtaatag
    22201 ggtgacaata tatcaaggag ctactttagg agctacagga aaagatactg gaaagagaca
    22261 tccaactgtt ggtgatgatg tattaattgg tgctggaaca aaaatactag gacctttaaa
    22321 tataggttca aattcaaaaa ttggtgcaaa ttctgttgtc gttaaggatg tacctaatgg
    22381 tgcaacagtt gttggtatac cagcaaaaat agtaaaaata agaaacctag aacctgtaaa
    22441 aaagaataaa aaagaagtgt cctatgagta tgatgaatta gataatgtat attatatata
    22501 ggatttatat aactataaaa taaactttat aaaaattatt ttaaaaggta tttttttcaa
    22561 atttgaggac ataataaaaa agaactgaat gttacagttt tggttcatca atctttgata
    22621 aacaagattt taaatttgta gcgtctagga ggattttatt atgaaagcaa atgttaatca
    22681 agatacttgt ataggttgtg gattatgtcc atcaatatgt ccagaagtat ttgatatgaa
    22741 ggatgatggg aaatcacatg ttatagttga tgaagtacca agtgatgctg aagaatcagc
    22801 agcagaagct agagaaagct gtccagttga tgcaatagat gtgcattaat aaaataaatg
    22861 cctataaaaa ttgaattttc aatttttata ggcatttgtc ttttattcag ttagtatttc
    22921 aacccctgtt tctgtaacta aaaccataca ttcccattga gcagataatt taccatcttt
    22981 tgtataaata gtccaattat tttcttcatc aacaaaaatt tcgtgagttc cttcatttac
    23041 cataggttct attgtgaata tcatcccagg aaccaatagc atctcagtcc cttttttaga
    23101 tacataactt acaaaagggt cttcatgaaa gcttaatcca actccatgac cacctatttc
    23161 tctaacaaca gagtatccat ttttctttgc ataagagttg atagcatcac ctatatctcc
    23221 taaaaatccc catggtttgg caacttcaag acctttttgg acacattcct ttgtaacttg
    23281 aactaatttt tttgcatttt cattaacatt acctatcata aacattcttg aagcatctga
    23341 atagtatcca ttaaaaatag tagaaacatc tacatttata atatctccat cttttaagat
    23401 aatgtccttg cttggaatac catgacaaac ttcattattt actgatgtac atacactttt
    23461 aggaaatcca ttatagtcta gtggagcagg gatagctcca ttttgtagtg taaagtcatg
    23521 aaccaactta ttcatatctt ctgtagacat tccaatttcg attttatctg aaatataatc
    23581 caaaattttt gtgtttatta cagcactttt ttttatttcc tcaatttgac tttcattttt
    23641 tataatatca tgagttggta caatatgacc taacttagca aatgaatcta ttttattatc
    23701 catatccata tgacattttt tatatttttt agtacttcca caccaacagc tatcatttct
    23761 atttaacttc ataacttttc atccttcttt tattatttat ttaaattatg ggttacataa
    23821 actattttaa aacataacca ttataaatat tataacataa atactaagaa atcctttata
    23881 gatagtgatt tattcaaagt ataaattatt atatggttat tgttgatgaa aagaggatag
    23941 ttaaatgtat tattggatat tagcgcactt aatagtttag ttcaagtttt tcaagaaaat
    24001 catatgttag aggacaattt tcctttaaag tattttttga atcttcatca agataataat
    24061 gcacaaatga ttctgcaaaa aattcttcta taaacttgct tatatatgca tgtgcaactt
    24121 gtttagggtc ataaagctta tatctttcct gagcaaatac ttccataaat tcttcagaat
    24181 ttgatttttt atttaaaaca tttttatcta tagcatgtgc agtttcatgt aattctaaat
    24241 tttttgatga atgtacattt gcatagctag ggttactata acctatttta gctactatag
    24301 aattatttct atatatacca ggtacatctt tccaagtctt gcctgatctt acccaacatg
    24361 gaggtaattg atattttaaa tcactaaatt ctggttcatc agttagattt gaatttatta
    24421 attttatttt tatatccttt tctttaagtg tctgtaaata tttaatattt atttttgcaa
    24481 gtctgctttt catattttca acttcatctt tatcataatt caagtcatca ggaagtataa
    24541 gcatttgttc aattatatct atgctactgt cttttatatc ttcagattct tcatcaaatt
    24601 tatattgaag agtattattt tcatagatag ttatatttgt attattttcc atataataag
    24661 agtgaatatc ctctaagtct aaaatattca tttgttgtaa aatatgtttt gtttcgccac
    24721 tccatactac taaagaaagt gaattaaaat taattagtgg attttccttt gcttttatat
    24781 cattgcttat atctacatca aatacatatt caattgagtc ttctatgtta agactatctg
    24841 tattcttttt aatatataaa tatgcttgtt ttaagtctat ataaatctca tctaaagatt
    24901 catcataaaa ttctgataaa ttattagtaa agagttcttc acttaaaagt tcatttttat
    24961 cagttatact ttcaattaaa atattaattt cttttatcat atcgtaaata cttatttttc
    25021 tatttgcttt ttcactagat acattgcatg gcgcaagagc aatgcaataa aaatcttttt
    25081 cttttttata aaaggcttta agtgattttg ataaaagtgt aaagtctaca cctatctgta
    25141 agttgacctt aagtgagtca ttttgtaata attcagtatt atttttttta gctatattta
    25201 aattgttttc cataatcatt gccccctttt tattagggta tcataatttt tttatatgtg
    25261 tcaatttgtt tttttgaaaa ttataatttt tttgaaaatt ttatttttta taatttgtga
    25321 attttttatt atagaaataa atatgttatt attatgtcaa aatttagcat tagattcatg
    25381 tagagtatgt tttatacata tattataatt aattctttga agattgcata ttcaaaatac
    25441 acaccatttt ttttaattct ataatataat atttatatat ttaatgttag gatgtgattt
    25501 aaggtgtatt ttacatatat tataagatgt aaagatgatt ctctatacac tggatatacc
    25561 agtaatatag ttagaagaat gaatgaacat aagttaggaa ttaattcaaa atacacaaga
    25621 gcaaagggat ttgaaaaact agaagtttat tttgtgacca acacaaaaag caatgctatg
    25681 aagttagaat actatataaa aaaattaact agaaataaaa aattgtctat aataaaaaac
    25741 ccaagtatac ttataaattt aattgataat aaagaagatt atattattgg taaggaaata
    25801 gaacaattaa cataaatatt tatagtaaca ttataatggg tgcatatagt gtaaaaactt
    25861 gacaataaat actattgtaa tataatgaag ttataaatat agtaaattaa aatagctagg
    25921 ggagccaaga gctgagaggt ataccgaccc ttacacctga tctggataat accagcgtag
    25981 gaaagcttag taaaaattca agtatgtatt atttgtgata tatgctattt ctaagctata
    26041 cctattgtag gtatagcttt tttaattaag ttatttaaat taaactttag gaggcaacaa
    26101 tgagtaatta taaaatacca acacttacaa tagctggctc tgattcatct ggaggagctg
    26161 ggattcaggc tgatttgaaa acgtttagtg caattggtac ttatggtatg agtgtaataa
    26221 cagctataac agcacagaat actaaaggag tttttgctgt agaggattta aataagaaaa
    26281 taataaaaaa acaaatagag gcagtctttg aagatatacc tccaagagct gtaaaaatag
    26341 gaatggtttc aagtcctgag attatacttg aaatagttga gaatctaaag aaatacaatc
    26401 caaaatattt ggttgtggac ccagtaatga tatctaaaag tggatattat ctattaaagc
    26461 cagaggcaaa agaaaactta attaagtatt tgataccact tgcatacata attacaccaa
    26521 atataccaga ggcagaagaa ataacaggaa taaaaataca taatgtagat gatatgaaga
    26581 gagtaggaga agaaatacta caacttggac ctaaatttgt acttatgaaa ggaggtcatt
    26641 tagatggaga agcagtggat attttagttg gtaaaaatat atttaaagtt tataaaagtg
    26701 aaagaattga taaaaaaaat acacatggaa cagggtgcac attatcttct gcaataacat
    26761 cttatctagc attaggatat gaaataacag aagcagtaaa tctaagtaaa atctatataa
    26821 cagaagcaat aaaaagaagt tttgatatag gtcatggagt aggaccagta catcattttt
    26881 ataaatttga gtaataaaaa aatattggag gtagaatatg tataatttaa ttaaagatgt
    26941 aaaaaaatta aatccattgg taattcatta tactaataat gtaactataa atgattgtgc
    27001 taatgtgact ttagctgtag gagcaagtcc tcttatgagt ttttcatatg aggaagtaga
    27061 agaaatggta agtgtagcaa attctgtagt aataaatata ggaactatga actcaaatat
    27121 gttggattta tttctattag ctggaaaagc agcaaataag tacaataaac ctgttgtttt
    27181 ggacccagtt ggagtatttg caagcaaagc gagagcagaa cttacaagta gacttttaaa
    27241 tgaagtcaaa tttagtgttg ttaaagggaa tgtatcagaa ataaagttta taggtggctt
    27301 taatgtaagg ggtaaaggtg tagattcatt tgatgaagaa gaagattcta ctgagataat
    27361 aagaaagatt gctgaaaagc ttgaatgtgt agttgtagca actggaaaaa tagatataat
    27421 aactaatggt aaaggtatat ataaaataaa caatggaact gataaattaa aaggaattac
    27481 aggaactgga tgtatgactg ctagtttgat tgcaagtttt atggctgtta ctgagaatat
    27541 attagaagca gcaacaatgg gtgttcttac aatgtcttta agtggggaat tagctaattt
    27601 aaataatcca ccaataggta catttaaaga aaatcttatg aatgctattt atcaaatgga
    27661 tatagatact ttaagtaaaa attctaatat tgaattttta aattaataaa gagtaaggga
    27721 gacttaaaat gattgataaa gaatctttaa aaaaatgctt aaagttgtat ttggtaactg
    27781 actctgaaat gcttaaaggt agagactttt ataaatgtct tgaagatgca atatcttctg
    27841 gaataacaac tgtacaactt agagaaaaaa atgcttctgg aagagagttt ttaagaaaag
    27901 ctatgaaact tagagaaata actgaaagat atggtgttaa gtttataata aatgatagag
    27961 tggatatagc acttatttgt gatgcagatg gagtacatgt aggacagagt gacattgatg
    28021 tgagagaggt tagaaagctt ataggaaaca ataaaattct aggagtatca gcaagaactt
    28081 tagaagaagc tacttgtgca aaaaatgatg gcgcagacta tttaggtgta gggtctatat
    28141 tcacaacatc tactaaatta gatgcaaaat cagcttcgtt tgaaactgtt aaagaaatta
    28201 aagaaaaagt agacatgcca tttgtgctaa ttggaggtat aaatttagac aatatagata
    28261 aacttaaatg tttagaaagt gacgggtatg ctataatatc agctatatta aaagctgaag
    28321 atatctctaa agaagtggaa aagtggacat taaaaatata aaattggctc tggtagtgaa
    28381 aaaaatggaa aaatttgata taatctaaga gtaaaattta agcaattata aatgataaac
    28441 ttttctacat atacttttta atcaagatat atgtagattt ggttaaaaag gagctataaa
    28501 tgattaagat attgttagtg tgtgttggag gcatgtcttc taccctattg gtaaataaaa
    28561 tggagaagga tgcaaaaaaa agaaatatag actgtaaaat atgggcagtt ggagaaggag
    28621 atataaaatc ggagcttgat aactttgata ttttacttct tggaccacaa cttagattta
    28681 tgcttgatga cgtaaagtct atagttggag atagagttcc agtatctata atagatatgg
    28741 taaactatgg tacttgtaat ggtcatgctg tgttaaattc ggttttagaa atattaaaat
    28801 aaataataaa agtggattct atataataga gtccattttt attatttgtt attaataggc
    28861 ttaaaactta gcttaccatt tcttttttta atctccacat agcaagtgaa aaacttgaac
    28921 agcacaatat taaaaatagt aggataacag gcagtatatc tccaataaga atagtctcac
    28981 caaatattaa gctacgtaaa gcgttaataa catgggtaaa tggattgagc atcactgcta
    29041 cttttaatcc tccagataaa ctttctatag ggaaaagagc tgaacttaaa aagaaaatag
    29101 gaagtacaat tgcagtcata attgtttcat aaactatttc atttggcaat aataaactta
    29161 tagaataagc tagacttgat aaaaagaagg ctgtcataaa tatcagtact atcataagta
    29221 ggattccacc aatgccagat tctatcctta ctgagaagaa tatacttact atacacaaaa
    29281 tagttacttc tatgaaggaa actaagatag cttctaataa ttgaccaagg acgattgaat
    29341 acctactgat aggtgcaatt aagactctat agaaacttcc actgttcttc attataaagt
    29401 ttataatacc accactacta caagaagaaa acacaaccag tacaatgata cctggtagta
    29461 taaaagcagt ataattattg atatttatat tattcatagt ttggtttgca atagaactat
    29521 aaagcacaag ccaaagaaaa ggttgtaata tagtaactac aaatgagatt ggatttttaa
    29581 aacgccactt catactacgc cataagatat ttaacattcc cattcctcct ttaagctttt
    29641 tttagaactt gtaagtgcta aaaatacatc ttcgagactt ggctctacaa tttctattgc
    29701 atcaaattct atatcatgtt ctaaaagcca tttatttatt aaagtaaaag cagttctact
    29761 atcgtttaca cttataaaaa ttgaattttc tcgtacgctc ataaacttta ctaaaccagt
    29821 acttttaatt gaatctttat atttttttat atcttcagta ttatggaaag ttatacgtag
    29881 tatattttgt ctaatgtaac tacgtaaact tgatggtgtt ccttgtgcta aatcctttcc
    29941 attcttcata atacaaatat tatcactcaa ttgttcagct tcttcaagat aatgagtagt
    30001 tagaaatata gttgtgccgt actcatctct aatttttaat aacatatccc acatagattt
    30061 tcttgaatcg acatccatac caacagttgg ttcatctaaa aataggattt taggagatga
    30121 taccatattc atagctatgt ccaatctacg ttttacaccc cctgaatatg atgaagtagg
    30181 atattttaag taactagata agtcaaactt atcaattaaa gagtctattc tttgttttgc
    30241 aatctgtggt tctactttat acattttact ttgaaatacc atattttcca taagggataa
    30301 atgttcatca atagagattt gctgtgctac acaagcaatt tgagtacgta tccaagaagg
    30361 attatctact aaatcttttc caaacatttt tacatttcca gatgtaggtt tgtaaaaggt
    30421 agtcaaaata tttataagag aagatttacc agcaccatta ggaccaagta aagaaaaaat
    30481 ttctccagca tttacattaa agcttagacc atttaaagct ttcacaccat ttttatattc
    30541 ttttactaaa ttgtctattt caatcgctaa catatattat tcctcctctt taagtggaaa
    30601 tattatctct gtaatatagt tatctgggtt tccttttatc aacatacctg gtcctttaat
    30661 aaatacttct ctccaaggag tttgtatttt tatattattt tcatgaatat atttaaatat
    30721 catttcatac gtttttggca aattactata agagcctgtg tgagtaaaaa ataaagcttt
    30781 tgttttagga aattcttttg tagttatacc catacgattt ggagtttcag cagttggaac
    30841 acataattcc atttctgcaa caccagtttt ttggtcaaca tcatagtagc aaaagaatgg
    30901 tgcaccgtta gactttcctt ttatagcttt aaatacattt ggaaaatact ttgttgcctc
    30961 agtcataggt ccattataac gcatggtagc cactgtgact gattcgatat ttttgatttc
    31021 aattttgcaa ttcatttgtt tacctcctaa tgtgtgttca tattcttact agatttttaa
    31081 gcattaatac atataagatt ttgattagtt gaaaacatat aatcttatct cgtttgcttg
    31141 ttttcaagtc taacataaca taaaatatat atcctctcat ttgatgctcc attttgtaaa
    31201 gctattattt aagctttact ttagattttt tagcaaattg aaataaagca atttataaaa
    31261 taagtattta caaattaagt gtattaatcg tataatacac ttaataggat actagataaa
    31321 gtctgaaata aaggcgtatt tttttaaagt aagtgtacta tcgtattaat acactagaat
    31381 aggaggaatc atgaattttg aattagataa tacaactccc atttatttgc aaatagtaaa
    31441 gtatataaag agacaaatag taacaggaga gttgaaacca ggagagacaa taccatcaag
    31501 aagagaaatg gcacttaatc taaaagtaaa cttaaataca gttcaaagag catataagga
    31561 gatgggtgat atgaatataa taaatacttt taaaaattat caaagcagtg taacagttga
    31621 tgaaaatata ttaaaaaatt taaaattaga gcttataaat gaatcactat ctgtttttat
    31681 agaagatatg aaagcaataa atgttagcaa agaagaagtt ttaaaaataa tagaagataa
    31741 gtattagttt tttaacaaat agaaaactta gtttagaaag tttatttata agcatattgg
    31801 atgtggagga tagtaaatat gatagaaata aagaatgtca gcaaaacata taaaaggatg
    31861 caagggctta aaataagaaa gatagaagct ttaaaaaatg taagttttaa tatagaaaag
    31921 ggaaaaataa ctgcattact tggtataaat ggtgttggta aatcaactat gttaaaagca
    31981 atagcaggac ttataaacat tgatagtgga gaaattcgta tagatggaga aaaaataaat
    32041 gaaaaggtat acaataaatt ggcttttgtt ccagatgtac aatctcactt tagcaataca
    32101 acaataaaag aaacatttga atttatggaa attttttatt caaagtggaa taaagaaaaa
    32161 tcaaaagaaa tgatggacat atttaaattg gatgaagatg aaataattga taacttgtca
    32221 aaaggaaaca ttgcaagagt aaaattaata cttggatttt gccaagaccc agagtacata
    32281 cttcttgatg aaccatttac aggaattgat ttgtttaaga gggaagagtt tataggagta
    32341 atagcgcaat atatggagga aaatcaagcc attatcataa ctactcatga gatagtagag
    32401 atagaaagtt tagttgatga ggttgtaatt cttgatgaag ggcagattat aacttcattt
    32461 aatgcagaag aattaagaga aagagaaggt aaatctatat tagataagat gagggaggta
    32521 tataagaatg aataaacttc tatatctaat aaatgtagat ttgagaagaa gcaataagtt
    32581 ttatatagct tatatatcat ttttttcatt aattacactt agtttaaatt tattcgaaat
    32641 taataaattt aaaaattcta aatttattat gaacttagca atagaagatt ttggaggtat
    32701 attttatgga ctagggatac ttagtaatct tggttttatc caaaatatat tgttctttgg
    32761 aatgttggga ctttttatat actatatttt tatgtggaaa agagagttct tttcaaataa
    32821 tcaaagtatt tatacactta tgatgttacc tcaaaataag tttaagattt gtatatctaa
    32881 atcaatagtt ttgataacaa tgatatatgg ttttttgata agtcagacag caacattatt
    32941 tatatgtaaa tatatattta atttcatttt tagaaatatt cctataatca atatgaactt
    33001 tgccaaagat ttatattatt taaacttgaa gagtatacca attgatttta tctcttttat
    33061 ggctatttat gtatttttac tacttatagc tatttctatt gtaaattgct gtatattatt
    33121 tatcttttcc tatataaata aattttatgt aattttaatc actttgctga ttatagcagg
    33181 cttatatttt tcaacaatat atataaatat aatcaattta tt
//
LOCUS       DAELXX010000032        32813 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.032, whole genome shotgun sequence.
ACCESSION   DAELXX010000032 DAELXX010000000
VERSION     DAELXX010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32813
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.032"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(113..952)
                     /locus_tag="KPH29_001473"
     CDS             complement(113..952)
                     /locus_tag="KPH29_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG1467092.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMFILIVVGAITGIL
                     GLF"
     gene            complement(976..1734)
                     /locus_tag="KPH29_001474"
     CDS             complement(976..1734)
                     /locus_tag="KPH29_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG1467093.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNENVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            complement(1781..2254)
                     /locus_tag="KPH29_001475"
     CDS             complement(1781..2254)
                     /locus_tag="KPH29_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG1467094.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSHNGDEHLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDVKCFIQIVPDAKKIDVGTLL"
     gene            complement(2283..2699)
                     /locus_tag="KPH29_001476"
     CDS             complement(2283..2699)
                     /locus_tag="KPH29_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG1467095.1"
                     /translation="MIHMTGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSRRLEDRIEKYKDEGVLVLLDFYGGTPFNTSAIIINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            complement(2746..3798)
                     /locus_tag="KPH29_001477"
     CDS             complement(2746..3798)
                     /locus_tag="KPH29_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG1467096.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGTIGLFSIQWAKIFGSTKIIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPMTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            4194..4874
                     /locus_tag="KPH29_001478"
     CDS             4194..4874
                     /locus_tag="KPH29_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBG1467097.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            complement(5007..5327)
                     /gene="sugE"
                     /locus_tag="KPH29_001479"
     CDS             complement(5007..5327)
                     /gene="sugE"
                     /locus_tag="KPH29_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBG1467098.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     gene            5709..6536
                     /locus_tag="KPH29_001480"
     CDS             5709..6536
                     /locus_tag="KPH29_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBG1467099.1"
                     /translation="MNFYKIAVCQMMTTENKIENINHAVDMVTEAAINGAKIIVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEYGKIYNTCYVFDK
                     NGALIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGVEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
     gene            7357..8031
                     /locus_tag="KPH29_001481"
     CDS             7357..8031
                     /locus_tag="KPH29_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1467100.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNGYEVYTAQSGKDSLKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREQRKSNQTKIKFYNDLTIDYEKRLICIYNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            8019..9179
                     /locus_tag="KPH29_001482"
     CDS             8019..9179
                     /locus_tag="KPH29_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG1467101.1"
                     /translation="MDKISNMSLKKAFFVLTLSSLIIASVLTTFAYILLNNLYNSIQD
                     KYLKTPINTGIITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNINMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEGKSFTLKSQINNINLF
                     FDENIILRVVENIISNAFRYARNNVSILIYLEQELLTFVIEDDGIGFSKESLKLALRP
                     FYRDKTLNDSNPHFGMGLHISKILCEKHGGSISIENNSTNSAKITAKFSTRN"
     gene            9396..9923
                     /locus_tag="KPH29_001483"
     CDS             9396..9923
                     /locus_tag="KPH29_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBG1467102.1"
                     /translation="MKSFANNITFSRIVFALLMLSSTFCSLPFWICYFWCGISDILDG
                     LIARKLRQQSGIGAKLDSIADFIFAVTILVTVGKNIALPMYLWWCIVAIALLRLISYS
                     IGFFKYRTFSSLHTLMNKITGISIFAFPLLYICFGLDVAGIMICIVAFLSSFEEIAIM
                     IKSKELNRDIKSIFM"
     gene            10209..10877
                     /locus_tag="KPH29_001484"
     CDS             10209..10877
                     /locus_tag="KPH29_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1467103.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLKINSGEFVSVIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKDYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            10880..13348
                     /locus_tag="KPH29_001485"
     CDS             10880..13348
                     /locus_tag="KPH29_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1467104.1"
                     /translation="MKNYLSLSIKELKSQKLMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITIGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGHLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGTSENLLPKRYILSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTVAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYMFPLVLSTNVSLI
                     FSFISALPSAIYASRVSPKIAMAGSTTKIKRKIKREKAIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSTLDKIDKM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSILEDFK
                     NGSICFIKNPIAISTPGVKTRHTNLKPKDSITINKKQLDIYSTVDKMFFLQGNGWING
                     VDVIVYNSVYNTLTNKNKFNQINIYAKDKSKLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNISLY
                     KMFLWEGVYYGIFAAIFGSIAGYASAIIINMATIEKLDFTNIPITSILQATIISVLAC
                     IIATLIPLRKVKKMNIIDCIDINL"
     gene            complement(13588..15012)
                     /locus_tag="KPH29_001486"
     CDS             complement(13588..15012)
                     /locus_tag="KPH29_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1467105.1"
                     /translation="MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDI
                     YPPNYYERQVPRIEKYINEKNITLLSQSNEEGLKRTIRGDDMPYQVVDNNGNILYGTN
                     PKKLFKTKEELFNNFINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANSRGR
                     FVFAVIIMALFSPFIYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMKGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNCISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(15031..15723)
                     /locus_tag="KPH29_001487"
     CDS             complement(15031..15723)
                     /locus_tag="KPH29_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1467106.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            complement(15778..16554)
                     /locus_tag="KPH29_001488"
     CDS             complement(15778..16554)
                     /locus_tag="KPH29_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBG1467107.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLCMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEA
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSY"
     gene            complement(16551..17321)
                     /locus_tag="KPH29_001489"
     CDS             complement(16551..17321)
                     /locus_tag="KPH29_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBG1467108.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQSLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIVVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTILEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            complement(17318..18022)
                     /locus_tag="KPH29_001490"
     CDS             complement(17318..18022)
                     /locus_tag="KPH29_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBG1467109.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTDGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDPNEN
                     LEEFFTDVILKGGQNR"
     gene            complement(18277..18789)
                     /locus_tag="KPH29_001491"
     CDS             complement(18277..18789)
                     /locus_tag="KPH29_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467110.1"
                     /translation="MKKISQEKISTHIQEVPQEKIEQWKSLYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKISSDKELKPIVFTIPNEGNAKK
                     LYDTQEETYSGCKIIVGMEFETGCVFVEGSDELNDEITSFKGLDKYDISNYYLVANYI
                     RCLKKYKLID"
     gene            19115..19372
                     /locus_tag="KPH29_001492"
     CDS             19115..19372
                     /locus_tag="KPH29_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467111.1"
                     /translation="MILNRTELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FSNEITLGNLGQKEDFSYEYLIYVHKDDYEQAESLINSKLR"
     gene            19515..20141
                     /locus_tag="KPH29_001493"
     CDS             19515..20141
                     /locus_tag="KPH29_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HBG1467112.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDNRIISYLPNITIETDKIKTIMIN
                     EVVPTNTNDDFYSVDKDADYLKTTIPLFDSAGIEVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(20391..21170)
                     /locus_tag="KPH29_001494"
     CDS             complement(20391..21170)
                     /locus_tag="KPH29_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBG1467113.1"
                     /translation="MNSKIYYFSGTGNSLVVSKILKETLNGSKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIIKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPSDVEEERLKNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNGCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLDK"
     gene            complement(21238..21699)
                     /locus_tag="KPH29_001495"
     CDS             complement(21238..21699)
                     /locus_tag="KPH29_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG1467114.1"
                     /translation="MGGAGDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            complement(21675..22352)
                     /locus_tag="KPH29_001496"
     CDS             complement(21675..22352)
                     /locus_tag="KPH29_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG1467115.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
     gene            22585..22971
                     /locus_tag="KPH29_001497"
     CDS             22585..22971
                     /locus_tag="KPH29_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG1467116.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVATESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            22974..24773
                     /locus_tag="KPH29_001498"
     CDS             22974..24773
                     /locus_tag="KPH29_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HBG1467117.1"
                     /translation="MYAPFFIRFLLSTLTLSILIVIILLAKKVFKKHISAKYQYNIGF
                     ILLIMLIIPFVPLKYINLGNVFNYLYTFNQLGSSTNRTSIIKNSPNSIINNSNALNDF
                     SVSLNQVNFDSLNILLLISWIIGILIMIAITIHCNIKIKNMKKSIQILRDEEIVFIFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNKDILINYIMCFLQILYWFNPLVWYAFKEMRIDREIACDISVLKKLDKDSHIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIAL
                     IVFGNIPKLSIMADINEKYRPTNEQTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALLGLENGIITRNNSHIPWDGKHHSNSSWNEDQDLFSAMENS
                     VNWYFQTLDKKTGLKNIQTYLKQIGYGNYDVSGGISNFWLESSLKISPIEQVELLESF
                     YTNKFKFEDKNIETVKDSLLISKKDGVSLFGKTGTANINGKDVNGWFIGYVEKNENTY
                     FFATNIQSKDYSNGSSASKITLSILNDKKIYNP"
     gene            complement(25128..27002)
                     /locus_tag="KPH29_001499"
     CDS             complement(25128..27002)
                     /locus_tag="KPH29_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG1467118.1"
                     /translation="MNKYNKIANELIEIIGENNIISITHCATRLRVMVKDREIINDKK
                     VEKVNEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEVEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIGLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVFSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDIA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(27007..28671)
                     /locus_tag="KPH29_001500"
     CDS             complement(27007..28671)
                     /locus_tag="KPH29_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBG1467119.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKDLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDATPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSKYSDCLIYGSIEPIDIESDNVIAYRRQLDLVVIDCY
                     YNFSDKLVEIKTEEKYDIVFQNLDDINLLNKTVSLKPFQAVLLKVR"
     gene            complement(28685..29506)
                     /locus_tag="KPH29_001501"
     CDS             complement(28685..29506)
                     /locus_tag="KPH29_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG1467120.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSIGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKKREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDVHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDIVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(29605..30369)
                     /locus_tag="KPH29_001502"
     CDS             complement(29605..30369)
                     /locus_tag="KPH29_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG1467121.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVIDDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVVKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDTSTIIDFAKEMRMKGTYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(30366..31115)
                     /locus_tag="KPH29_001503"
     CDS             complement(30366..31115)
                     /locus_tag="KPH29_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG1467122.1"
                     /translation="MRLESLVNQSYNSLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KSDLFIIISLSGETKSGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            complement(31619..32224)
                     /locus_tag="KPH29_001504"
     CDS             complement(31619..32224)
                     /locus_tag="KPH29_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG1467123.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMKIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMMKSIRELDNLKYEWICMSHGTPCIK"
     gene            32403..32501
                     /locus_tag="KPH29_001505"
     CDS             32403..32501
                     /locus_tag="KPH29_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBG1467124.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFD"
BASE COUNT        11628 a         5441 c         3980 g        11764 t
ORIGIN      
        1 ctattataaa attaccctaa ttgctaaact ttttaatata aattttccaa catcttacaa
       61 atataatcaa tagatataga atcctaattt acacaccaac atatatattt atttaaaata
      121 gtcccaaaat acctgtaata gctcccacta ctatcagtat aaacattatt tttattggag
      181 aaaggccttt ttttaaagca aagagtacta ataaagtaag taccaaagga attaatcctg
      241 gaaatatctt atcaagtaaa tccgtttgaa gactaaattt aaaatcacca ctagtataac
      301 ttattggtgt agataaatta ataaacctac ctacaagtcc ccccatgact aaagtaccta
      361 atatagaagc cccttctata accttattta ctattccacc ttgaagtata ctttcaacag
      421 cttttttccc atacttatat ccatttgtcc acattccaaa agaaattgaa gtcattatta
      481 tatattgagc tattacaaat aatataggac ctgcaattga tgctcctccc tcagttaaac
      541 caatacaaac agctaacact ataggagtta taactccttg agttaaagta tcaccaattc
      601 ctgctagtgg acccatcaag cctgttttga tagcattcat accatctcca cttatttcat
      661 ctgctccatt agctttttct tcttccatag ctattgttat tccatgtata acacatccac
      721 atataggttc tgtgttaaaa aatgctaaat gagtctccac ttcttgtttt aactcttcct
      781 tatctggata taatttttta agtactggca acattgaatg tgagaaagct gttgattgca
      841 atctttcata attgtaatta gattgcgcaa agaaaaacca tcttaaccat gattttatta
      901 catctttttt gcttaacgtt ttcttagata cttcattttt tatatctgac ataatatttt
      961 ccccctcaca tgtatttaag atgtagcact atcttttccg tacttaattg aagtaattgt
     1021 aagagcaagt attactccaa taacagatat tactattata tctactttaa aataaacagc
     1081 cattagtatt cctaatatga aaaagggcat agtaaacttg tttccaatca attttaaatt
     1141 catagcaatc ccaagtgctg gcaacatagc cccaattaca ttaaatacat gtaacacatt
     1201 ctcattcatt acttccaaca taccatctat cgcatttgga ccaaaataaa caactaataa
     1261 tgctggtata aaactcattg caaataacag tatctgtggt ggtactatat ttataaatgc
     1321 tactctatca acattccctt tttgaacctc tctatcagcc caatgtgcaa aaaaagaatt
     1381 tactgtcatt tttccaatcc aaagtacagt tgcaacagtt cccaatggta cagctattgc
     1441 cattgcagta cttacatcta gcttagatgc tatagctaaa gcagtaccta catatcctgc
     1501 aaaagctggg tcccccatct gagctccccc tgctgatata aaacctagat atatcatatt
     1561 tatacctgct cctataattg ctccttgaac agggtctcct aatataattc ccgttacaaa
     1621 tccagccaat agaggtctat tccatgtaaa aaatccaaca taggtcaacc acggactcca
     1681 agaaagataa taaaaaattg ctattaatac tgcttgaatt agtgaaatgt gcatattctt
     1741 cctccctatt ttcttttaaa gatttacaag atagtctttt ttataataat gttcctacat
     1801 caattttctt tgcatctggt actatttgta taaagcattt aacatctttt gatattagtt
     1861 ctctaaatat ttctttttct tcatctgaca cagatatatt tttataaaaa ctttttctac
     1921 caagccctgc tcccatacct ccaacattca attcctttaa atctacccca ctatttacta
     1981 aacctaagac agttttaggg tatttgacca atactattaa atgttcatct ccattatgtg
     2041 attttaaaac ttcagcacct gattctacat catgtacttc aaccttaaca gttgatggag
     2101 ctgccatttg taaaacttga actataaatg ggtcctttac tactaaatca tccacgatta
     2161 ctatcctttt tgccgatgta atcttacacc aagcagtaat aacttgccca tgtattaatc
     2221 tatcatcaat tcttgttaat gctatattac tcataattat tcctccagat ttttaattta
     2281 ttttacaact ttaaaacagt tttaacatct ttgattcctg atatcccaac ttcctcacat
     2341 aaattcttca agtcttttaa cttcatagta tctctgttta aaaatagctc taaaagcata
     2401 ggtaaattta cacctgtcaa acattctaac tcacatacct tagcaaattt atttattata
     2461 atagcagatg tattaaatgg agtcccgcca taaaaatcaa gcaatactaa aaccccttca
     2521 tccttatatt tctcaattct atcttctaac cttctactaa actcatctat gctgtcacca
     2581 tggtttaatc ctaaagtttc tacattttcc tgtttgccta ctattaattc agcactttct
     2641 aacattgctt tagccatatt tccatgtgtg gctataatta tccctgtcat atgaatcata
     2701 cctttcttaa ccttgtttat atggattatt atttagtact ctaatctaat tatctatatt
     2761 aatcattatc ttaccaaaga aaatatccct tttgtatact ttttcaaaat aagctggtac
     2821 ttcttctaaa tttattctat gtgttactaa tttttcaaca ttcatatcac ccttttgcat
     2881 atgatacaat ccagcactcc attcttttcc tgggaatgga aaagagtttc caaaccaagt
     2941 tcccttgaca gttaattcac ttcttactat cttttcaaat tgttctctag tcaaagctac
     3001 atccccatat ggtacacctg catacaatac tgtccctccc tttttagcta acaacaatac
     3061 ttgcccacat gtcattggag tcccagccga ttcaataaca atatctgcac catctccatc
     3121 agttaacctt tttatttctt ctacaatatt cttttctttt gcatttatac aaatatctgc
     3181 ccctaactct ttcgccaagt ctaacttctc atcaaataca tctacagcta ttatttttgt
     3241 agagccaaat atctttgccc attgtattga aaataatcct attgtacctg taccaagtac
     3301 tacaacagta tctccaactt tagcttctga tctaaaaaga ccatgccctg ctatacatac
     3361 tggttctagc gcagcggcag tctcataact tatctcatct ggtattttta ttaaattcct
     3421 ctctttcacc tttgtatact ctgcaaaaca acctccaagt tctttatttc ctattattgc
     3481 tacattatta catcttgaat acaatccttt tttacattca tcacattcaa aacatggcat
     3541 agctggacaa acagcaactc tatctcctat tttaaaactc cttacttctt taccaacctg
     3601 tgccacttct ccagaaaatt catgccctaa aatttctcca accatatgtg gacctgtctt
     3661 tgaatatttt gatatatcag aaccacatat tccagctact tttactttaa ttattacatc
     3721 atctttttct aatatttttg gtacatccac atcctccact cttgtgtctc ttataccata
     3781 gaatctaact gacttcacta ttaaccaccc ctacttttta tttgagaata tatccctatt
     3841 gtttatatgt taaatatcta aagcaacttt catgccaata tatgtaagca ttgcattcat
     3901 gcattttata aaattatcat taaatgtgtg tcaaagtgtg tcaaaagttt cttatattaa
     3961 aacttatgtg tgtcaatata aacgacacta acattttata ttagttctct tattataagt
     4021 tttataaaat gtactttttt tgttattttt ataaaattgt tcggaaattt cccttctatt
     4081 tgtgatataa tattaaatat aaggtatcaa tataaatttt catgcgaata tcagaacttt
     4141 tttgacacta atgccttatt caatataata atttaacttt ttggaggttg tttatgaaaa
     4201 aagtgtatca aggaaagact aaagatgttt ttgaattaga taatggaaat tacttactta
     4261 aatttaaaga cgatgtaact ggtaaagatg gagtatttga cccaggcgaa aatagtgtag
     4321 gtctaagtat agatggaatt ggtagagcta atttagagac atctgttaag ttctttgaaa
     4381 ttctaaacaa tgctggaatt aaaactcatt atgtaagtgc taatttagaa gaagcaacta
     4441 tggaagttct accagcaaaa gtttttggta atggattaga agttatttgt cgttatagag
     4501 ctgttggtag tttcttacgt cgttatggtt catgtgttga agaaggtgct aaactagatt
     4561 gctatgtaga agcaacttta aaagatgatg accgttgtga ccctcttata acatctgaag
     4621 gtttagaagc tttaaacatt atgactcaag ctcaatttga ttcaatgaaa agcatgactc
     4681 aaaaaatttc taatatagtt cgtgatacta tagctgaaaa aggtcttgaa ctttatgata
     4741 tcaaatttga atttggattc tacaatgatg aagtaatctt aatagatgaa atagcatctg
     4801 gaaatatgcg tgtttataaa gatggtgtaa tagttgaccc tatggattta actgctcttt
     4861 tattagataa atagtatata catataattt tcaaatataa taaacttata aataagataa
     4921 gctatgaagt aaagactttt taaatgttga ttatttttaa acattttctc gcctttaatt
     4981 tcatagctta tttaaattaa tatactctaa ttagtagata atagctttaa ccctacaatt
     5041 cccaccacaa taaagcctat acatgcaatt cttgatatac taataggctc atttaagata
     5101 gccatgccta caatagctgt accgacggta ccaattccag tccatattgc atatgaagtt
     5161 ccaagtggaa gatgttttag tgctaatgac aaaaaataaa aacttacaat cattccaaca
     5221 acagtaagta tacttggagt taatttagaa aatccatgtg aattttttag ttgaaaagcc
     5281 caaaacactt caaacaaacc tgcaacaaac aaaaataacc atttcataaa ctcatccctc
     5341 ttttcaaatg tatctcttat ttttccaata aaataacctg agaaatatta taaaatatct
     5401 cccaggtttt tgtccttccg tgttcacaac aaaaactatg ttgtgggttt tatctcggac
     5461 caggccaatc attaataatt gcaaaaccct ataaaactct attaattatt ctattttatc
     5521 tataataata ctatcaacat atataaaaaa agtcaatata tgggttccat ctataataaa
     5581 aataactttt atataccttt taatattctt attcacttct ctccataccc atttaggtca
     5641 atctaagaat atctaatttt tatttaatat gttataataa cataaaaata ttgaaaggag
     5701 taatttctat gaatttctat aaaattgcag tatgtcagat gatgaccaca gaaaataaaa
     5761 ttgaaaatat caatcatgct gtagatatgg taacagaagc tgccatcaat ggagctaaaa
     5821 ttatagtttt gccagaaatg tttaactgcc catatgaaaa taaatatttt cctaaatttg
     5881 ctgaagaata tccaggtgaa acaacaacga tattgagtaa attagctgag aaacatggaa
     5941 tttatctagt ttctggtagt attcctgagt tagaatatgg aaaaatctat aatacttgct
     6001 atgtatttga taaaaatgga gctctaattg gaaaacatcg taaaatgcac ttatttgaca
     6061 ttgaagttac aggaaaagtt agttttaaag aatcagatac tcttacagct ggaaatgatg
     6121 tcactgtaat agataccgaa tatggaaaag ttggtatagc tatttgctat gatattcgtt
     6181 tccctgagtt atcacgttta atggctctta aaggtgttga aatcgttatc ttacctgctg
     6241 catttaatat gactactggt cccgcccatt gggagttatc tataagaatg cgtgctttag
     6301 ataatcagat attctatgtt ggtgctgcac ctgctagaaa tatgaatgct tcttatatag
     6361 cttttggaaa ttcaagaatt tcagaccctt ggggaagaat aattgctcaa gctgatgaaa
     6421 aagaatgtat tatttatgct gacattgata gagatttaat tcctgacata agacaacagt
     6481 taccacttct taagcacaga agaactgatt tatatgaact taatactttg aaataaaaca
     6541 gaacataacc ccataaatca taaattaagt gttaaaaaaa tttatggggt tctcattaat
     6601 ttgatttaaa attaatttaa taaaaataat gctaaaatta atttaattaa ataatactaa
     6661 taattactaa tgattatgct aattgtcagc atttactaat ttaatcacta ttagttattt
     6721 cttttattaa atatctttac tttttttact tattatgtct tctagcttta catgtggatt
     6781 atggtatttc tttctatttt tagtcacatc tttctattga attgcttgct taaaacacca
     6841 atgcacacat gccagataat gttcacaatt atgtaaaaat attgctctac cttcttcaat
     6901 gttgatatta tctctcctag ttaatattat ctttcctaaa atttctttta attaaaaaac
     6961 aacattagtt gatatatcaa gtatatttta agcaactgaa aaaataataa taattgatat
     7021 atcaactaat taatgtagat taattcattt ttataattta tatacttttt aattaatcta
     7081 cctttttaat taaaatcctt cctttctatt tatccatttt caacttaaat tataagattt
     7141 catcagctgt gtttttgcat tccaataaat attaaatatc aaatatatat tctattaaaa
     7201 tttctaataa tttcatcctt gatttattta atatataaaa atatactaac tttaatgatt
     7261 aagttttgaa atataactaa gaatttgaga atttattgag atttttatac taaaatataa
     7321 atactataca aacttaattt agaagggagc attcttatga acaataaaat actcgtcgta
     7381 gatgatgagg aaggtataat aacactctta aaagattact ttgaaatgaa tggttatgag
     7441 gtttacactg ctcaaagtgg aaaagactca ttaaaaaaaa tatccaaaaa tccagatatc
     7501 attctacttg atataaacat gcctgaaata gatggaattg aagtttgtaa tagaattaga
     7561 gattatgtat catgccctat tctatttctt acagccagaa ttgaaaatac tgacaagata
     7621 aatggttttc gtgctggtgc tgatgattat attgtaaaac cttttgattt agacgaactt
     7681 ggagctagag tatctgcaca tttaagacgt gaacaaagga agtcaaatca aactaaaatt
     7741 aaattttata atgatttaac aatagattac gaaaaaaggt taatttgtat atataataat
     7801 cagatttcac tttcaaaaaa ggaatttgat atagtagagc ttctctctat gaatcctgga
     7861 caggtatttg ataaagaaaa aatatatgat agagtctggg gatacaatag caatggaaat
     7921 agtgatgtta taatggaaca cattagaaaa atacgaatca aactatcaaa acatactctt
     7981 gaaaattata tagaaacagt ctggggagtt ggttataaat ggataaaata agtaatatgt
     8041 cattaaaaaa agctttcttt gttcttactt tatcttcact aattatagca tcagtactta
     8101 ctactttcgc atacatttta cttaataatc tatataattc tatacaagat aagtatttaa
     8161 aaacacctat aaatacaggt attataacca tagttcaaaa caatgtgaac caatttagtg
     8221 attatgataa aacattactt aatataatca acatacttca gcttgtactt ccacttatat
     8281 tttttatagg acttttatta ttagctgata taatttttta caaggtaaaa ttaaaaacac
     8341 ccatagaaat cctcaataaa ggtgcaatgg aaatatccaa caacaattta gatttttgct
     8401 tagaatataa taataatgat gaattaggta atctatgtaa tgcttttgaa aaaatgaggt
     8461 ctgaactaaa taaaaacaac ataaatatgt ggactatgat taatgataga aaacaactta
     8521 atgctgcttt ttctcatgat ttaagaaatc ccctaacagt tctaaaaggt tactcaaact
     8581 atttaacaaa atacatacca acaggcaaac ttagtgatga gaagatttta tcaaccacac
     8641 aacttatgtc tgagcatatt aacagaattg aatactatgt agaaaatatg agtaatgctc
     8701 aacgattaga agatttagtt gtttcaaaat ctatgtcgaa tataaacaag tttatagaaa
     8761 acttagatga aaatatatct atcattgcta aacaagaagg aaaatctttt acacttaaaa
     8821 gtcaaataaa taatataaat ctattttttg atgaaaatat cattcttaga gtcgtcgaaa
     8881 atataatatc gaatgctttt agatatgcta gaaataatgt atccatactt atatatcttg
     8941 aacaagaact ccttaccttt gtaattgaag atgatggtat aggatttagt aaggaatctc
     9001 taaaattagc tttaagacct ttctatagag ataagacttt aaatgacagc aatccacact
     9061 ttggtatggg tcttcatatc tcaaaaatat tatgtgaaaa acatggtggc tctatttcta
     9121 ttgaaaataa ttctactaac agtgctaaaa ttactgctaa attctcaaca agaaattaaa
     9181 atgattttgt aagctttcat tgttcttata taagctttgt tagtttagaa tttataaaat
     9241 gtatttcaat ttacaaccaa tatatatact attttgtaga agttgtaaga gttgcttcaa
     9301 ttataatatt ttggcaaaag gcatttgaaa caataataga aatatatatt taaatatgta
     9361 gaatagtatg aaactagaat tttggaggtg attatattaa aagttttgct aataatatta
     9421 cattctctcg tattgtgttt gcattgctaa tgcttagttc tacattctgt tcactgcctt
     9481 tctggatatg ttatttttgg tgtggaatta gtgatatact tgatggtctt attgcccgaa
     9541 agttgagaca acaaagtgga ataggtgcaa agttagatag tattgctgat ttcatttttg
     9601 cggttacaat tttggttact gtcggaaaaa atattgctct tcccatgtat ctatggtggt
     9661 gtattgttgc aatagcatta ttacggttga ttagttacag tattggtttt tttaagtatc
     9721 gcacattttc atcgcttcat actttgatga ataaaatcac tggaatttct atctttgctt
     9781 ttccattact ctatatttgc tttggtcttg atgttgcagg aattatgata tgtatcgtgg
     9841 cttttctttc ttcttttgaa gaaatagcaa ttatgataaa atcaaaagaa ttaaatcgtg
     9901 atataaaaag tatatttatg tgacaactta gaatttgtga caaaaataat ttatataata
     9961 atgaggaagt aaaacataaa aaattatcca caagtttatg tgtaaaatag ttttaaagca
    10021 atagagttgt attgtaaagc ctttagtgca taaattacat tttaaattaa aaatgatact
    10081 gaaactgctt atcagcactg cgaattatcc gtaaatagtg aagacttttt agatttaagc
    10141 taaaagttgg gaaaaaattg agttttaaaa cttacactct tcttatatag aaaaggagag
    10201 tgttttttat gattataaaa gcaaaacaat tatcaaaaat atatggttct aataacaata
    10261 aagttatagc acttaataat gtaaatttaa aaataaactc aggtgaattt gtttctgtaa
    10321 ttggaccttc tggaagtgga aaaagcacac ttttacatat tttaagtgga ttagataatc
    10381 ctacctctgg acaagtgtta cttgatgata aagatatata taaacacact gaaaaagaac
    10441 tatctgcact tagaagaaaa tcctttggat tcgtctttca acaatttaat cttttacctg
    10501 ttcttacagc atctgaaaat atttcaatgc ctgtacttct agataaaaaa caacctgata
    10561 aagactattt aaatgaaatt tcatcattac ttggaattgc agatagactc aatcatcttc
    10621 cccatgaact ttcaggcggt cagcaacaaa gagttgcaat cgctagagca cttattgcaa
    10681 aaccagatat aatatttgct gatgagccta caggaaactt ggacagtaaa agtggcagtg
    10741 aagttatgaa tcttcttata aaaacttcaa aacagtttgg aaagacttta gtggtaatta
    10801 ctcatgatga tagaattgca aaacttgctg atagaaaaat ttcaattatt gatggtgttc
    10861 ttatggaggt gaaatagaaa tgaagaatta cctttcactt tcaataaaag aattaaaaag
    10921 ccagaaactt atgactacat tcatcattat agcaatagta ctatcaacta ttatgacaac
    10981 tgtggttgga cagtcaattg gtatcctaca aaatttaaga attgaacaag cccgttcttt
    11041 taatggtgat agacatgtat cctttcacca acttactaaa aatcaagttg atgacataaa
    11101 gaaagatgat agagtttatc aagctggtac ttcaataact attggcagta gtaaaataaa
    11161 ggattctggt atctctgtac ttgtaaaaga gtatgataaa acaggacttt ctaactaccc
    11221 aaaattaatg aaattaaaat ctggacattt gcctaaagat aaaaacgaaa tagccttaga
    11281 tgaaaacact ctaaagctta tgggtataaa accaagacta ggagtgacta tacctatgaa
    11341 tttagatatt tcacttttaa atgataccat accaccatat aattacacag ctaactttaa
    11401 attgagtggc attttagagg atgattatac aggatatgta agtggtatag taaatggtat
    11461 tgtaggtaaa ggtacatcag aaaatctatt acctaagaga tatattttat cctctttgga
    11521 ttttaaaata aaacagcaag aaaagtttca agaaatagta aatcaattag ctaaaaaaat
    11581 aaatctttct cacaattcaa ttcaatataa ttggatttac ttaaatgctc ttaagataca
    11641 gtttgaaaaa gatgaaaata gttctaatag tgatggtata tctatgatta tattggtatc
    11701 tttatttgtt gccttattgg ttttactggc ttctggactt gtaatctata atattttaaa
    11761 aatctctgtt acaaagaaaa ttaaagagta tggatgttta agagcaatag gagctgaacc
    11821 aaaccagata tataagatag ttattttaca aatcttaatt ttatgtactg ttgccatacc
    11881 tattggagct gtaattggaa ttatctcttc taaaggcata actggaatgg tcactaatat
    11941 cttaaatcca gatatacttc tagcaaatga taataaagaa attactgagc ttatacataa
    12001 aaatacaaca gcatatatgt tcccattagt attaagcact aatgtatctt tgattttttc
    12061 atttatttca gcattaccat cagcaatata tgcttcacgt gtctctccca aaattgctat
    12121 ggctggtagt actaccaaaa tcaaaagaaa aattaaaaga gaaaaagcaa taaaaaactt
    12181 tgaaagacat ctagcatggc taaacttaaa aagaaataaa ggaagaacta taataactat
    12241 actatcatta tttatgagta taacagtatt cgttgcattg agtgagttct ctaatgtatt
    12301 agatgtatct aggtcagtaa gcaatttaaa agaaggagat ttttccttaa caaatgaaat
    12361 atctggtttt gataaatcaa cactagataa aatagataaa atgaaaaacg tcaatcgcac
    12421 ttcctttata aagtactctg aatacaaaca gggtgaaata gatacagata taaattttga
    12481 aaatagtggt gagatgttaa aaatcattgg aattgacgag cagactttaa aagacttaat
    12541 gccttctatt acagattcta tactagaaga ctttaaaaat ggttctatct gttttataaa
    12601 aaatccgatt gctatttcta caccaggtgt aaaaactaga catacaaatt taaaacctaa
    12661 agatagtatc acaatcaaca aaaaacaact agatatatat agtacagtag ataaaatgtt
    12721 ctttttacaa ggtaatggct ggataaatgg tgttgatgtt attgtatata attctgttta
    12781 taacaccttg acaaataaaa acaagttcaa ccagataaat atttatgcta aagataaatc
    12841 aaaattagaa caaataagat tgtctattga acagatttgt gaaaataatc ctggctctca
    12901 ttggatatca tatattgaat cagataagca acttaaagag agttttaaac aaatagaatt
    12961 tctagcatgg gcagttatat tatttgtggg attgattggt actttaaaca tcattaacac
    13021 aactcataca aatataaata ctaggactaa tgagattgga gttaaaagag ctattggtat
    13081 gagtaatatt agcttatata aaatgttttt atgggaaggt gtttattatg gaatttttgc
    13141 tgctatattt ggttcaattg ctgggtatgc cagtgctatt attataaaca tggcaactat
    13201 agaaaagctt gatttcacta atataccaat tacttcaatt ttacaagcta ctattatttc
    13261 tgtacttgca tgtattatag ctactcttat tccgttaaga aaagttaaaa agatgaatat
    13321 aattgactgt atagatatca atttataact attttacaga taataatata aattaaatta
    13381 tcaatgactg tctctatttt ctttcaaatc taactaataa atctttttaa ctataatata
    13441 agctttaaaa aaggcctgtc tcaaaataga gataagcctt ttttcattgt cggaataatg
    13501 aaaatatatt taacttcaca aaaaaagagt gcctcacgaa ttaaaaattt cgctttgaga
    13561 cactcccctt taatttaaaa tatatagtta aatgctatgc caaaatttca cacatgctcc
    13621 acctgatttt gaattttcta ttttaacaga acctccaagc tgttccacta attgtttgac
    13681 aatgtaaagc ccaagaccag aatgtccacc ttttgtctgc ctagcttcat ctcctcgata
    13741 aaacttatca agagcctttt ttaaatcttt agagctaaac cctctaccag aatcacaaat
    13801 ctcataagaa atacaatttt tttcatcctt tacagtaata ctaatattac cacctgatgg
    13861 agtgtattgc agactgttgg atatgatatt atcaaaaatt cgtgtcaacc tatccacgtc
    13921 gatttgaatt ggggattgta tattcccctt cattttcaat ataagttcaa cttccttttg
    13981 atgagcctgt aattcatagt cttgcacctt ttgcctcaaa aattcaggta aatttatagg
    14041 aacaagattt aattgtatat ttgacttttc taagtcagac gtatattgca tcttttggac
    14101 aagagcagca cttttttctg tattttctct tatcactgta agatacctat gaagtttttc
    14161 attgtcatct gtattattac caataagtgc atctgtatat cccaaaataa tagaaagagg
    14221 agattttaaa tcatgtgcta gagcttccac catttcaaca cgttcctgct ccatcttcca
    14281 ctgcgcagat aaagaacctt ttaattcgtc tttcatttca gaaaaggcac tacaaagttt
    14341 accgagttca ttatctgaat aatagtcgat ttcaaaatca aggtcctttt cttttatctt
    14401 cttggacgca tcaatcaata gatgtaatgg ttgattgata ttctttacaa agcgcttaga
    14461 aagccatcta gtaaaaccca cgatatatat aaatggagaa aacagagcca ttataataac
    14521 tgcaaataca aatctccctc tactatttgc aaaggttatt ttgacttgat agaatagaat
    14581 aacagctccc tcaatctttc cattatcccc tttgattgga acagtatgga tataaccacc
    14641 tttacgaaca gttttattga taaaattatt aaataattct tcttttgttt taaatagttt
    14701 tttggggtta gtaccatata gaatattgcc attattatcc acaacttgat atggcatatc
    14761 atccccacga atcgttcttt ttaaaccttc ttcattcgat tgtgataaca aagttatgtt
    14821 tttttcattg atgtattttt caattctagg aacttgtcgc tcataatagt ttggtggata
    14881 gatatcctta tttaaggaat gaatatacaa atagagagca aatacataag taatcactgt
    14941 ggcaactata ctggctatta taataaaaat aaaagtatgt ctaaattggg taataaggga
    15001 ttgatttttc atttaataac ctctctctct ttacatttta ttccacttat acccaatacc
    15061 ccaaactgtg gaaatatatt ctttttctgg tgtcacagca gaaaacttag ctcgtatttt
    15121 ttttactcgt tcaacaacag ttgaggaatc accttcagaa tcacaccccc atactttttc
    15181 atagatttgt tctcttgaga aaacttgtcc tgcatggagt gctagtaatt caacaatatc
    15241 atactctatt tttgttaaat caatattctt accatcaatt ttaattgcac gttccttcat
    15301 atataagcat aacttcccaa aatacaaagt agatctcttg ttctcttcat tgatatactg
    15361 tgaacgcttt tccctacgca gattagcttc aatccttgcc ataagttcac gcagtccaaa
    15421 tggctttgtt acataatcat cccctccaaa ggtcaaccct ttgattttgt cggtttctga
    15481 ttgctttgca cttaaaaata taattgggca taaaacatca tctctaattg cctgacatac
    15541 ctcaaatcca ttcattttag gcatcataat atccagtata ataaggtcag gctcctgttt
    15601 tgataactct atacctgcct gaccatcata agctatcaac acttcatgtc cttttgcctt
    15661 caattcatcc tcaagtagca ttactaggtc catttcatca tcaataatca atattttact
    15721 caatataaca ccacctttct cttatgggaa ggtcagtatt aaaacaatat tcgtctacta
    15781 ataacttttc cttccctccc acattttaaa ccaaataacg cctcctatca agaatataat
    15841 caaacctatt gctactacta taagctctat actagctgtt tgcatcactt tagagtaaaa
    15901 tgcctcagtt gaaatagttc cagcttcttt aataaaataa attcctggaa gcatacctag
    15961 ttttactggc catgtccatg gaacaaatgc ccatactttt tcaccaacgc tagttaatcc
    16021 aagtattgct gccatcaaaa ttccaccgat actaatacca atagatgcac ccatacccca
    16081 tgcaaaactc acccaaagat gaatagctaa tagtggaagt gtcccaatca ccacaagtcc
    16141 tgctgctgac aggaataacc atattgttgc tccagatggc actgcaatac tcattcctat
    16201 gcaaagtatc aacgtagcaa taaatgtgca tatagttagg caaaacagaa gaaaaagaaa
    16261 ttttcccaga tataatctaa cacgagaaat acctacacat aaaaaaccat taaaatttcc
    16321 agcaagctct tcctcctgca ctataaatcc tgctaaaata cctactccaa taggaatgat
    16381 gaatacagtc catactgtaa aaaatccttc aaaagcaaat tcttgtgtac ttccagattt
    16441 agttgtgaga taaaaaacca cacataatga aaaaataata ggcatacaaa gagtaagcca
    16501 ctggacagct gtcctctttg ctcgaagcca ttctgatgat aatagcttta tcatttgtca
    16561 tccctcctat tgaaccaagc tgcagtaagc gctgttaata ctataaacac aaccaaagaa
    16621 acaacaatcc caataggaat cactgaagta ttcaaaagtg ggtctccagc ttctaatata
    16681 gtaccgttag gatgtatgtt gatgattggg cataccattc taactgccca actccaaggt
    16741 actgcaatcc aaaatggttt tgtcgcagca aggacaccaa atatcatacc taaagctcca
    16801 actcccatac ttaggaacat acctttccat gtagctagcc atagctgaag tggaagtata
    16861 gcaagagaaa caacccagca tacaatactt gcccctataa tctgtccaaa aggaactgga
    16921 ccagctttca atattaaacc agttataatt gttacgacta ttaaaactaa cgttgaaata
    16981 aaactgtaga ctgccatagc tataacttta ttaatccaaa gtgtcatagg agacacattg
    17041 tgtgtacgta aagcacgata atttccagct tttttttcct ggaaagctac caaagtagca
    17101 aacaatgcaa atccaatagg cagaaataaa aatgaccacc agttaaaaat taatgctaaa
    17161 agactttgcc acgcccaaga agttgtttga gataatggat tttgcatgag tgttgattgc
    17221 attataaatg catatgctgc aaaagacact gggaaaaaaa caataagttt acccataaat
    17281 gttcttttat actttaaaag ttctgattgt aaagcattca tctgttttgt cctcctttaa
    17341 gaataacatc tgtaaaaaat tcttctagat tttcattagg gtcaggcatt ccttgaaata
    17401 gtagtcgtcc tccatttata attcctatat tatctacaac ttgagcaacc tctgatagaa
    17461 tatggctaga aagaattaca gttatacctt tttttggaaa agaagcaatc aattcacgca
    17521 attcttgaat gccaaagggg tcaagaccat tggttggttc atctagtatt aacaactctg
    17581 ggtcactcaa aagtgcaata gctattccta acctttgttt catacccata gaaaattgtg
    17641 aagctctctt tttacctgta tttttcaaat ttactatttc caacacttca taaattttat
    17701 ctttaggtat tcccaatatt tttgtatgta ctaacaaatt ttcctctgct gtaaggtttc
    17761 catacaatgc aggggattct attaaagaac ctatttttgc aagagattct cgtttccaag
    17821 gctttctatc aaaaataata cgaccatctg ttgggcgaag aagaccaaca agcattttaa
    17881 gtgttgtgga ctttcctgct ccatttggtc ctaaaagtcc atatatacta tttcgttcaa
    17941 tttgtagaga aacattgtta actgccattt gttctccata tctttttgat aagttttttg
    18001 tttcaagaat taaatctttc atattaattt ctcccttctt gtaatacctt aaatctatta
    18061 ctaagagaat actaacatga aaaaataaca attctataac acctactaat tttaataaaa
    18121 attaaaataa aaaatagagt taccaatttt attttggcaa ctctatttaa gtttatgttt
    18181 tacagttata ttagatttaa gcttagtttc attatcacac taacttcaaa tttactctat
    18241 aatccttaaa tcaagcacta atttattaat caaacactaa tctattaatt tatatttctt
    18301 tagacatcta atatagtttg ctactaaata ataattactt atatcatatt tatctaatcc
    18361 tttaaaagaa gttatttcat cattaagctc atcactacct tctacaaaaa cacaacctgt
    18421 ttcaaattcc atacctacaa taatcttaca gccactataa gtttcctctt gagtatcgta
    18481 caattttttt gcatttcctt catttggaat tgtaaataca ataggtttta attctttatc
    18541 actggaaatc ttttgtgaaa aaggcttgtt taacttaata ttattgataa ctacatgttt
    18601 aaattcctct gaagtttctt ctactattac atattttcgc tttaaaaact ctattatttc
    18661 taaaacagac ttagaattta cttttaaaat acttttatat ttttcatata aagatttcca
    18721 ctgttcaatt ttttcctgtg gtacttcttg gatatgtgta cttatttttt cttgagatat
    18781 ttttttcaat taaactctcc tttcaatact taaatatcct tataaatctc acatgactaa
    18841 agttacaagg ttcctactta atagaatttt gcatacttaa atatgtctta ttaattctct
    18901 acaggatatc tccgtagtcc ctacgattct tacataagtt acctatttta aattgttatt
    18961 agttagatta taaaaattat ttattttatc actaatataa tactcttaaa aactttacaa
    19021 atcaatttaa atttgctacc ttttatttat aacaacttat agtacatttc tcctatgctt
    19081 ttcaatatat tacttttaaa aggaggtttt ccttatgatt ttaaatagaa ctgagctttt
    19141 ccgaggatat tctttaaata gatttaacga gatatgcaat atattatcag taaataaaat
    19201 taaatataag tataaagtga aaaataatgc ttactctaaa aaaccttttt ctaatgaaat
    19261 aacacttggc aatttaggtc aaaaggaaga tttctcttat gagtacttaa tttatgttca
    19321 taaagacgat tatgaacagg cagagtctct aataaattct aagttgcgat agattattta
    19381 taaaacatga cattctgttg tcatgtttta tatgttatta tactttcaaa tatgctacta
    19441 tattttcaaa attgtgtgct ttaaaactag ctttttatag taatttaaag cttaaaacta
    19501 aggagactcg ttatatgaaa ataaatataa aatcaaagtt aagtgaattt attaaacaga
    19561 acaatctttt tgatgacaac agaataatat cctaccttcc taatataaca atagaaacag
    19621 acaaaataaa aactataatg attaatgagg ttgtcccaac taacaccaat gacgattttt
    19681 atagtgtaga taaagatgct gattatttaa aaactactat tcctttattt gacagtgcag
    19741 gaatagaagt atctaacata aatgatatcc tagatatggg tatctatata actaatgcag
    19801 taaaacttcc taaatcagaa tatacaataa ctcgtgatac aattaaatta catatgccta
    19861 ttttagaaga agaaattaaa ttatttaaaa acctagaggt agtaatgtta atgggagatg
    19921 ttgcaaaaaa atcttttaat atgattacta aaaagcatat aaaaaagaat gtcatccctt
    19981 ccatttcaac atataaactt cgtaataatg aactttatta tgataattta agagtatttc
    20041 catcatatat tatgactggt ggaaacatac tgattgaaaa atcaaaattt gaaatggcat
    20101 cagaagatat aaaaaaaatg ttcgagatta taaacggttg atattacaaa ataataaaaa
    20161 ctaatttagc caaataataa gtgcctcaaa aacttctatt tatgaggcac ttttatttta
    20221 tattagtgta actatatact attaacataa tttattattt atattgttct ttttaatctc
    20281 tattccattt agtctaggta acattactct ttacaagatt actttatatt tcttttatat
    20341 ctctaacgtt tttatttaat aaaacaatta ttaatacaag tcaaatatat ctatttatct
    20401 aaaaatatat cttttacagt aatatctgga tggtgatact tcttcctatt cgttaaaaaa
    20461 gattttttta cattaatagc ctcttttgga caccagtgaa aacaagctag gcatctttca
    20521 cactcattac cccactttgg ctttttatca actatactta tattctctac aggacaaacc
    20581 cttttacatg tcccacatcc attacattta ttttctgcaa aaaatttagt agttgcaatt
    20641 tgcatattct tacctactaa acgaagtaca acactatcta tatgtgactt taaattgttt
    20701 tttccttcta tatttccaat ctccaagtta ttgatacatt ttgcaatctc agctacccta
    20761 ttagtctgat ttttcaatct ttcttcttca acatctgaag gtgttatcaa agcatttcca
    20821 ggcatattga ttaaaaaacc agcagataat tttttccccc tttttttaag taacttatct
    20881 atagtaaata aactatgtcc agctacccca ttacaagtgg caatggcata tatatgagta
    20941 ttaatattaa aatttaattt ctctacaaaa gactttatta tatcaggaac atcagcaaaa
    21001 taaacaggaa aaacaattac caatatatct tcatttatgt ctatattatt ttcttttcta
    21061 tgtatagcta aaggtataac ttctttactt ccatttaacg tttcttttaa aatctttgaa
    21121 actactagcg aatttcctgt tccagaaaaa taataaatct tactattcat tcttatccct
    21181 ctttctatat ttctacatca tcaatagtta aaaagtctct taaaatagga tttatattta
    21241 ctttccaatt ctatccaaaa tatcagtaaa caaaggcaat tcttcatcag agatagattt
    21301 aaatgtctct gttgcatata tacttgcaat ttcatttaat ggttcttcta attctctacc
    21361 taaatctgtt aggaatattt gaaacttacg tctatcttta acatctactt tacggacaat
    21421 taatccctta tttttcataa cgtctaaaat tcttgttgta ttagatttat ccttacatga
    21481 tttttcacat aaatctatct gagaaattcc atcttgctcc catagaaacc ttaataacgc
    21541 ataatggtca gttgttatat catatccatt ttgtttaaac atctttttta aatcatcttt
    21601 tacttttaac gaagtactta ctaacttagt tgtagcttca tatagtttct caaaatcttt
    21661 cattttacct ccatttaaat aatcccctgc tcctcccatc tatcaattat tcccttatat
    21721 tgctcaatat ctttaaaagc cattggcttt aaaacagcat ttatagcaat aaaattatag
    21781 atttgagaaa gcacaggtgg atttttcttt tcctctatgc aataaataaa ttttttagat
    21841 accttttgta tctttgattc tactttttca gttataggat tttgattaaa tccagttttt
    21901 atagataaac taccacaaac ataacctccc gaatctaaga ccatggattt tataaaagaa
    21961 attgcttttg aaccacgtgc attttcatat gtagtaactg ctatacatgg cttacgatac
    22021 aataatcttt caagcatcat atgtgctcta tctgtaaaca ccttgaaaag tccagttaca
    22081 ctacttccat aagttgggct accaaatata acaccatcac tcttagtaat tatatcatga
    22141 atatactcta ctttatcatt ttgattgata cacttgccca ttttataaca atttaaacaa
    22201 ccaatacaat atgatggatt tacctcactt aaatcaaaat aattaatttt tacatctggt
    22261 attaatcttt ctatatcttt atacaagtaa gttaaaactt ttgatgtagc tccatttttt
    22321 ctagggcttc catttataac tgtaattatc atttctatat ctccttttta gttgttatat
    22381 caacaataag tatatcaact atatttttag ctgtcaatat aaaccattta tttaaagcta
    22441 tacttacaca gcttaaataa aagttttatt taactacatg tgcttattta atcaactata
    22501 aatgaattat ttttaataat tgacaaacta aattaatgat aatatacttt atgtattaca
    22561 aatgtaagta gaaggaggta tagtatgaaa aagttaccac atatttcaga agcagaatat
    22621 caagttatga aaattatttg gaaatatgct cctatcagca ctactgaagt aattgaaaag
    22681 ttggtagaaa caagtacatg gagtcctaaa acaattcaaa caatgttatt gagattagtt
    22741 aaaaaaggag ctttaacata tgaaaaaaat agcagagtat tcgtttatac tccacttgtt
    22801 aaagaagaag aatacgtagc tacagaaagt agttcttttt taaatcgttt ttataatggt
    22861 actcttaatt ctatggtact aaacttttta gaaaatgata aactatctga agatgatatt
    22921 gaggaactga gagaaatctt aaataaaagg acgactaagg gggataaata aatatgtatg
    22981 ccccattctt tatacgattt ttactcagta cattaacctt atcaatatta attgttataa
    23041 ttttacttgc caaaaaagta tttaaaaaac atatttctgc aaagtaccaa tacaatattg
    23101 gatttatatt gcttataatg ttaataattc catttgtccc tcttaaatat attaatcttg
    23161 gaaatgtatt taactattta tatactttta atcaacttgg aagcagtact aataggactt
    23221 ctattataaa aaatagtcca aattctataa ttaataactc aaatgcatta aacgattttt
    23281 cagtatccct aaaccaagtt aattttgata gtttaaatat tttattacta atatcatgga
    23341 taattggaat tttaatcatg atagctatta ctattcattg caatattaaa ataaaaaata
    23401 tgaaaaaatc aatacaaatt ttaagagatg aggagatagt atttatattt gaaaagtgta
    23461 aaaaagatat tggtataaat aaggatttta tactatgcaa atcacctatt ataaatacac
    23521 ctattacatt tggatacttt aaaccacaca ttatattacc agataagtct atatctaaat
    23581 tatctttaaa agatataaaa tatatcttac ttcatgaatt acaacacttt aaaaataaag
    23641 atatactgat aaattatata atgtgcttcc ttcaaatatt atattggttt aatcctcttg
    23701 tatggtatgc atttaaagaa atgcgtatag accgtgaaat tgcatgtgat atttcagttt
    23761 taaaaaagct tgataaagat agtcatatag aatatggaca aactctaata aactttgtgg
    23821 ataatatttc aagaccatca aatcttacac ttacatcaga tattggtggg tcaaaaaaac
    23881 aaataaaaaa gcgactattg aagataatgg attttcataa tgaatcaaat acgttaaaat
    23941 tcaaaagcat tttaattttt acattgatag ctttaattgt atttggaaat attcccaaac
    24001 tatctattat ggcagatatc aacgaaaaat atagacctac aaatgaacaa actgtgtacg
    24061 aggacttaag tcagtatttt aaaggttttg atggaagttt tgttatgtat gatttaaaat
    24121 ccaagcaata tcatatttac aataaagaaa aaagtaaaac aagaatttct ccaaattcta
    24181 cttataagat ttatagtgcc ttattgggat tggaaaatgg gataataaca agaaataatt
    24241 cacatatccc ttgggatgga aagcatcatt ctaatagttc ttggaatgaa gaccaagact
    24301 tattttctgc tatggaaaac tccgtaaact ggtactttca gactctagac aaaaaaacag
    24361 ggctaaaaaa catacaaact tatcttaagc aaattggata tggcaattat gatgtttcag
    24421 gtggtatctc aaacttttgg ctagaatctt cattaaaaat atcacctatt gaacaggtag
    24481 aattacttga aagtttttat acaaataagt ttaaatttga agataaaaat atagaaacag
    24541 taaaagattc attgttaata tcaaaaaagg atggggtatc actttttggt aaaacaggca
    24601 ctgctaatat aaatggaaaa gatgtaaatg gttggtttat aggttatgtg gaaaaaaatg
    24661 aaaatactta tttctttgct acaaatattc aaagcaaaga ttattctaat ggtagttcag
    24721 catctaagat aacattatcc atattaaatg acaaaaaaat ctataatcct taatatattt
    24781 actgtacatt ctaaaaaatt cacataattt tcatattaat tatcgattaa attcatatac
    24841 aaggcttatt atataaacat agaagatagt aaaactagct taaaaacata atataatacc
    24901 ctatacaaat agtggcaact ctccctcaca tcctcaattg ttgccactat ttaatttttt
    24961 gtatgaagtt aaatatattt tcatcatttc aacaatttgg gtgcagtaca atctctattt
    25021 tgagacaacc tctttaattg ttttagagtt tatgaattat ttctcatact ttaaaaagat
    25081 tgaattactc ttgataatct tcttttgtag tcattacata tcacctacta ctcaacacat
    25141 attctttgac ctatttttac atccttgttt atatccaaaa tatttacctt cttaccatct
    25201 ggaaacaata ccattgtcaa agtagacttt cctaattcat gtattctctt taaatcaaca
    25261 ttaacaagaa catctccctg tttaaccata tctccttgtt taacagcaat atcaaagcca
    25321 tctccattta atgcaactgt atcaattcca acatgaatta atacttctcc attagttcct
    25381 ttaatcccaa atgcatgtcc acttgaaaat actgtttcta atttacctga aataggagaa
    25441 acaatttttc catctataat atcaatagca aaaccatcac ctaacatttt tttagaaaat
    25501 gtttcatctg gtacattttc taatgcaaca attttcccct tagctggaga cacaaagttt
    25561 tcattatttt cattaaacac taattcttcg tcttcatctt catttatagc ttctttaggc
    25621 actccaaata aacatgttac aataaagcag aatgcaacca caaatattga tactaataag
    25681 tatgtgatta aattatatcc atcatagata tacattaaat aagaaggtaa aactgctaat
    25741 ccatatgaat tagattgaat atgtaaaatt gataatattg ctgctcctaa acctgaagat
    25801 gcacacataa taatcaaagg ctttaaatta tatcgaagct gaatcccaaa taaaacaggc
    25861 tcacttatac caaaaagttg agatagcatt gctccaactg cagtttgttt aacttgatta
    25921 tttttagatt ttttcataaa tgctagacat gttccaatat tcgcaaatcc atacattgca
    25981 caaagagtaa taagtgcatt aaatcctgta tttgctaata gtgatgtttc aatcattaca
    26041 taagtatgat gtaagccagt aataacagct aatggatatg taaatccaat aataaatcct
    26101 ccaattccta aaggtaaacc aatcaataaa ctgatgatat caaccatttt taattctatt
    26161 gtatgcatta taggccctac acctaacaaa ataactaaca ttgttattag cataacaaag
    26221 aatggtgtga aaataaggtc taatacatta ggcatatgtt tacggaacca tttttcaaga
    26281 ttagctccaa taaatgctgt aacaatagct gttaaaacac ttccttgaca gcctacaata
    26341 ggaataaatc caaatgccat aattgcttca acttcatttg atgggtcagc aacagaatat
    26401 gcattaggaa gtattggaga aactaacata agtccaataa caagtccaat aacaggtgtt
    26461 cctccaaata ccttaaatgc tgaccataca ataattgctg gtaaaaaagc aaatgctgtt
    26521 tctgtaagaa ccgaaacaag tgtctgaata tacaatggaa tatttgcaga agacattcca
    26581 aataatccaa gtacattatc attaaacaaa caacccttta atcccaagaa taatcctgtt
    26641 gccgcaataa ctggaataat aggaacaaat atatctgcta gagttctcat aactttctta
    26701 aatccagttt cattattttt aactaattca tcttgttctt tttttgataa agttttcaat
    26761 cccatttttt caacttctgc ataaacttta ttgactattc ccgttcctag aataatttga
    26821 tactgtccag aagtaaagaa aacaccttta acctcattaa ccttttccac tttcttatca
    26881 ttaataattt ctctatcttt aaccatcacc cttaatcttg tggcacaatg tgtaatagaa
    26941 ataatattat tttcaccaat aatttcaata agctcatttg ctattttatt atatttattc
    27001 atttttctac ctcactttca ataatactgc ttgaaatggt ttcaaactca ctgttttatt
    27061 tagtagattt atatcatcta aattttggaa tacaatatca tatttttctt ctgtttttat
    27121 ttctacaagt ttatcagaaa aattataata acaatcaata actaccaaat ctaattgcct
    27181 tctatacgca ataacattgt cactttctat atcaataggt tctattgaac cataaatcaa
    27241 acaatcagaa tacttactat cttgtctcaa ctttatcatc tttttataaa attctaaaat
    27301 actttctgaa ttgttaatct gactctgtgc attaatatct ttataatttt caataacctt
    27361 taaccatggt gttgcatctg aaaaacctgc attttcttta tctgcccatg gaaatggcgt
    27421 tcttgcatta tctcggcttc ttaaattcac aaattctaac gcttcttctt ctgagtaacc
    27481 ttcatctatt gaacgatagt attggtcaat gctagataaa tcattaaaat catttatggt
    27541 atctctttta aaatttgtca ttcctaattc ctgtccctga taaataaatg gtgttcctct
    27601 taaaaagaaa tacattgctc ccaacatttt tactgcatca tcattccttt cgtattgcaa
    27661 aagatacttt gttgtagctc ttggttggtc atgattttca ataaaattag ctgcccatcc
    27721 atgttcctga acatacattt ggctatctaa aatgcatttt cttaaatctg gaattgtcca
    27781 tgaaattctt ttaaaccact cactaccaga agcaacatct aagtcagcat gtttaaagtc
    27841 aaagaccatt gaaaagaaac catcttctcc aataaaatca cctagttggt catagctaat
    27901 acctgctgct tcagcaacag tcatacaatc atatttttta aacgtatttt cttttaattc
    27961 ataaagcatt ttttctattc caggctgatt tctaactgtt tttgtacatt taacaagacc
    28021 atcaacacca tcactttcta aactttgaaa acttaaattc tttttgataa atgtaatagc
    28081 atcaactcta aatcctgcca ctcccatttg agaccaggta ttaatcattt ggtaaagttc
    28141 acttcttaat attggatttt cccaattaag gtcaggttgt tttttaccaa aagaatgaaa
    28201 ataatattcg tctctattct ctaccttttc ccaaacactt ccaccaaaaa cagacctcca
    28261 gttatttggt aaatcgtttc cttttttaaa aatataataa tcatgataag gactttctgg
    28321 attttttaat gcttcttgaa accaaggatg ttcatcactt gtatgattaa gaactaaatc
    28381 taatataatt ttgattcctc tatctttact ttctttaatt aactctttta aatcttccat
    28441 acttccaaat tcacttgcaa tatctaaata atcactaata tcatacccat tatcgtccat
    28501 aggtgattta aaaattggac ataaccacaa tgatgtaata cctaaatctt ttaaataatc
    28561 taaacgagaa ataattccct gtaaatctcc aattccatca ttattactat cttgaaagct
    28621 tcttggataa atttgataaa cgatttcttt ttgccaccat ttcttttcca taaattactc
    28681 ctttttatat gatatttctt ctttttaatt ctttataaat tccattatcg aatatgtcct
    28741 cacatataga tgtcgctata tctttaagtt gttgatgact atttttcatt gcaattgtaa
    28801 cgtctgatgc ttgaaacatg acaatatcat tttgaccatc accaaaacat atggtttctt
    28861 tttgagttac ccctaatctt tcttgtaatc tcttaattgc tttgccctta tgaaaatctt
    28921 tttgaataat ttcataatac tgactagaaa tatctctctg tgctaattcc attttatctt
    28981 gtagtatatc tttcacttcg tcaaatacct tttcatttga ccataaacaa attttatgta
    29041 catcttgtga cttatactct tcaatattat tttcatatgt tattttttct tggatatgtt
    29101 gtttatttat acaagaatta gttcctttca actgattcat tgtttcaaat atttctttgg
    29161 ctttttgatt catgaaaact ttttcctgac tctcaatcga aaaagcaact tctctctttt
    29221 ttaacaaaca aacaacttcc tttatcaatc tttgattaaa agattgatta taaagtaatt
    29281 caccatgata ttgaatgtaa ttacctcctc ctgcaatata tccatcaacc cctaatgata
    29341 aaacatcatc ttgtattgta cctatacttc ttccagtaca aataacaaca ctgcaatgat
    29401 tcttttgaca taaacgtata gcgtgctttg cagattctgg gattccatat acctcatccc
    29461 taagcgtacc atctatatca agtattaata atttgtacaa tttcatcttc ctttcttact
    29521 atcaaaatca taatagcatt caaaaaaaaa gatatcaata ttttgatatc ttttaaaaaa
    29581 tatgttcatt tttagcacaa attttcactt tcggggaata tgttcacttt ctaaatacat
    29641 cttgtattta atataaagca gttcaattaa aataaaataa ggtgttgtta catcgtagtc
    29701 cagttgttta tctacagaaa tagatataga ttgaatatac aggttttcat cacataatct
    29761 tgctaaagga ttacttatca tttttgtaat agaaaccata taagttcctt tcattctcat
    29821 ttctttagca aaatcaataa ttgtagatgt atctccattc aatgagataa agaaaatcat
    29881 atcatctgtt tttataaaat gattaagcgc ctgaatcata tcataaccat aaacatcata
    29941 tataattttc cctgttggta agaaaatcct tttcatctct ttaacaactc tttcctgaga
    30001 ataaccttct ccaaagacga ttatacgatt tgcctttttc attctttcaa acataatcga
    30061 acaatccttc ttctctatat catctatcac cttattataa caatccatta attggtgttt
    30121 actttgattt ttatattcta cttctgtttc atttaagcgt ataataactc ttaattcact
    30181 atatccaggt aattgtagtt tttgagcaaa acgagaaagt gcacttgtag atatatggta
    30241 tttgtctgca aattcatcta tagaaagttt aatacaatct tctttatgat taagaataca
    30301 ctcacagata tacttatcat ttgctgtaaa tgaattataa tatttattga aaagtccttc
    30361 aattttcatt cttttctcct tctatactct atgtaattca tatgaagcaa atctaataga
    30421 gcataaaatg ctcctacgac ttcataagat atagcttgta atgtatcaag tttttgagtt
    30481 tcaacataca gattatactc acaaagtgtt gcaattgtat tattatcaag cctagttaaa
    30541 gaaatcgtat gtatatgatt gttgataaat tttgcaattt caattcctga ttttgtttct
    30601 ccagacaaag aaatgataat aaataaatca cttttttgaa atgattctct cataaattca
    30661 atttcaccta tatcaaataa gtcaataaca catttaccta ctgaaagaaa tattctcttt
    30721 atttcttgag ctaaactttt ctgttcatta cctgtcccat aaatataaat tgtttctgca
    30781 tcatatatta ttttacaaat atcttgaaat gatatttttt gtaattcatt cattaatagt
    30841 cgataattct cgcaaacatc atgaaaatta ttatcattat taacaaattc tagttgatta
    30901 tttttaagta aatactttaa atcagagaat gaatctaatc caatttttct acaaagcctt
    30961 aataaagtag ctctagaaac attacattca tttgcaattt tttcacacga aaaattacgg
    31021 tattctcttt gacaaatcaa tatttgttta ataataattt tatcattttc atttaaacta
    31081 ttataacttt ggttaactaa cgattctaat ctcatttttt ccactcccta aatattattt
    31141 taatatacat tagttgcttt ttctagtgaa gagtcttaaa taataaggat aaaataatca
    31201 tttgatttta tttttattaa aataaaaata acctgctaaa agcaggcacc aaaaagtact
    31261 tatcctttcg aacatgtact ttagatattg ctttcttcgg aagcttcatc tcttcatact
    31321 ctaaaataga gttttttcta actcaaaagt attttccatg gtagaaatta ctttaattca
    31381 tctgccattt attttttatc ttatttaata attactatta cttgaatatt tacttcttta
    31441 agtaaatttc atagttaaca aagctaaaac tattattata gatgttaaaa tacaaaatta
    31501 taagtattgt tataaatatg cttacagcta caaatataca tgatatatta aaaagaagct
    31561 gtaaattatt atcatacata caagcaacta tgcctctatt aatccatgtt aatttttatt
    31621 atttaataca aggagttcca tgagacatac atatccattc atattttaaa ttatccaatt
    31681 ctcttataga tttcatcatc tttttataat cccaattcca tggattagga taaggaacta
    31741 tatttccatt cttatttttg actaaatcac ctgcaaataa tacatcttca aaaatcatgc
    31801 agacatgccc tggagtatga ccaggagtat ggataatttt tatattccca atcttcatat
    31861 tctctccata aggttcaaca tccttaatga gccttctgga aattgccttt ccaataaact
    31921 tcttaaaccc aggtctatcc ttctctccta taataaaagg tatatcctct acatgagccc
    31981 atacctttgc tcctgttttc tctttcaata atttaatatt agatatatga tcaacatcat
    32041 gatgagttaa taaaatatgc ttaatatctg taagtttaat gccaagtgtt tttaattctt
    32101 taattatatt tttccccata aaaggaagac ctgtgtctat aagaataatt tctttatcga
    32161 acacaataaa acaatttgaa ccacgagtac tatctaacat taatacattt tcttttatct
    32221 tcataaataa attcccctta tttggtattt tacctatata attaattaat aaagttataa
    32281 atagttataa tacttgatat taattttatt ttttattaaa gtatactata ggtatacaaa
    32341 tattatattt acataagtat accatcagta tactttttta tcaatagaaa ggaataattt
    32401 atatgtcaga attatcaaaa agagataaag aaaaaataca aagagaaaat gagattattg
    32461 ataaggcaga aaaattattt tgtcttaatg gatttgatta aaaaatagct acgcacactc
    32521 gtgactttag tcatgagtta gtagctaaaa tagtcagcat atagggaaac ctatatgtag
    32581 agataggata acacctatcc aatactactt gaattgctgg aaacccctaa agctaaccaa
    32641 actaaaacat aaggatgaaa taaacctaag tgtgaaagtt acgaaagtag aaaaaattgg
    32701 ttagatggta taaggttaaa tcctaagtac tttaaaatgg gcaatcagca ggtaagctcc
    32761 gaataggaga aacttcaacg actattcctc ttgagggaag tacactacaa gct
//
LOCUS       DAELXX010000033        32460 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.033, whole genome shotgun sequence.
ACCESSION   DAELXX010000033 DAELXX010000000
VERSION     DAELXX010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32460
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.033"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..337
                     /locus_tag="KPH29_001506"
     CDS             <1..337
                     /locus_tag="KPH29_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBG1467125.1"
                     /translation="NKTDLLYLKNVVSNIVPSRDEFITIYKQALIKKEIDLDMVNIRE
                     TFNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLIRILPKPQKKLDLNESLILNNLKN
                     LEKQYNSSY"
     gene            350..862
                     /locus_tag="KPH29_001507"
     CDS             350..862
                     /locus_tag="KPH29_001507"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBG1467126.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            936..3143
                     /locus_tag="KPH29_001508"
     CDS             936..3143
                     /locus_tag="KPH29_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBG1467127.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            3168..3617
                     /locus_tag="KPH29_001509"
     CDS             3168..3617
                     /locus_tag="KPH29_001509"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBG1467128.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNASRPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            3632..4246
                     /locus_tag="KPH29_001510"
     CDS             3632..4246
                     /locus_tag="KPH29_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG1467129.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVSDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            4337..5893
                     /locus_tag="KPH29_001511"
     CDS             4337..5893
                     /locus_tag="KPH29_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HBG1467130.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNANNNIESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIENMNLEFLDGFDSSKTIEEFLNRLDSESK
                     RNFKKLCKENIKKSMFVVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDKKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKETKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEELNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            5883..7145
                     /locus_tag="KPH29_001512"
     CDS             5883..7145
                     /locus_tag="KPH29_001512"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBG1467131.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEEANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            7219..9006
                     /gene="aspS"
                     /locus_tag="KPH29_001513"
     CDS             7219..9006
                     /gene="aspS"
                     /locus_tag="KPH29_001513"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBG1467132.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMPTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTKMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDIQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
     gene            9465..10697
                     /locus_tag="KPH29_001514"
     CDS             9465..10697
                     /locus_tag="KPH29_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBG1467133.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMKCFGIKDK"
     gene            10711..11517
                     /locus_tag="KPH29_001515"
     CDS             10711..11517
                     /locus_tag="KPH29_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HBG1467134.1"
                     /translation="MGRKVKIGIIQQHSVLGDVKKNIEKAVEMIDDLGKHGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(11629..12498)
                     /locus_tag="KPH29_001516"
     CDS             complement(11629..12498)
                     /locus_tag="KPH29_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG1467135.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
     gene            12869..14296
                     /locus_tag="KPH29_001517"
     CDS             12869..14296
                     /locus_tag="KPH29_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HBG1467136.1"
                     /translation="MKLTKSISSMLCLALVLSLSPVAYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSSSVSNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIASVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFLNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVKIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGHLSYGSKVETIEIFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
     gene            complement(14372..14641)
                     /locus_tag="KPH29_001518"
     CDS             complement(14372..14641)
                     /locus_tag="KPH29_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467137.1"
                     /translation="MKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMKGISINTLLI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(14644..16086)
                     /gene="nhaC"
                     /locus_tag="KPH29_001519"
     CDS             complement(14644..16086)
                     /gene="nhaC"
                     /locus_tag="KPH29_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBG1467138.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAATKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDILNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            complement(16111..17277)
                     /locus_tag="KPH29_001520"
     CDS             complement(16111..17277)
                     /locus_tag="KPH29_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG1467139.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLDRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIISPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKDYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESVLVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIK"
     gene            complement(17502..19622)
                     /locus_tag="KPH29_001521"
     CDS             complement(17502..19622)
                     /locus_tag="KPH29_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG1467140.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVVDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHIARKILSINANEMIDSFVGDCIKDINFQNILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERAADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLGK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSDNIKKYQFNIGKDKNIMKYDYNFKRSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQDFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            19855..20256
                     /locus_tag="KPH29_001522"
     CDS             19855..20256
                     /locus_tag="KPH29_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467141.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQVGK"
     gene            20299..20592
                     /locus_tag="KPH29_001523"
     CDS             20299..20592
                     /locus_tag="KPH29_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBG1467142.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            20607..21047
                     /locus_tag="KPH29_001524"
     CDS             20607..21047
                     /locus_tag="KPH29_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HBG1467143.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(21139..21612)
                     /locus_tag="KPH29_001525"
     CDS             complement(21139..21612)
                     /locus_tag="KPH29_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG1467144.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(21612..22400)
                     /locus_tag="KPH29_001526"
     CDS             complement(21612..22400)
                     /locus_tag="KPH29_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG1467145.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            22728..23972
                     /locus_tag="KPH29_001527"
     CDS             22728..23972
                     /locus_tag="KPH29_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBG1467146.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGIHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLINKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALVTSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            24295..25392
                     /locus_tag="KPH29_001528"
     CDS             24295..25392
                     /locus_tag="KPH29_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-diphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBG1467147.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEITIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHVNHSTKKNILIMGGGLGLLPKSEQFYKELNSL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDATLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            25383..26081
                     /locus_tag="KPH29_001529"
     CDS             25383..26081
                     /locus_tag="KPH29_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG1467148.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKEVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     RNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            26084..27148
                     /locus_tag="KPH29_001530"
     CDS             26084..27148
                     /locus_tag="KPH29_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG1467149.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFAMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITAMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            27331..28035
                     /locus_tag="KPH29_001531"
     CDS             27331..28035
                     /locus_tag="KPH29_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1467150.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            28028..29350
                     /locus_tag="KPH29_001532"
     CDS             28028..29350
                     /locus_tag="KPH29_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1467151.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            29537..30910
                     /locus_tag="KPH29_001533"
     CDS             29537..30910
                     /locus_tag="KPH29_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467152.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEEFNGKSKIATILVALGFLADIIVMYIFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(31283..32221)
                     /gene="pdaA"
                     /locus_tag="KPH29_001534"
     CDS             complement(31283..32221)
                     /gene="pdaA"
                     /locus_tag="KPH29_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBG1467153.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDGGKDTSNVIVSDGTDKPSKATTNNDNNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTA
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
BASE COUNT        12270 a         3887 c         5455 g        10848 t
ORIGIN      
        1 taataaaaca gacctattat atttaaagaa tgtagttagt aatatagttc catcaagaga
       61 tgaatttata acaatttata aacaagcact tataaaaaaa gaaattgatt tggatatggt
      121 aaatataagg gaaactttta atgtaattcc attaaagttt tttacgatat taaatgtatt
      181 tagagaatta aatttacttg actttaattt aaattatgaa aaaaattctg tactcataag
      241 aatattgcca aaaccacaga aaaaattaga cttaaatgag agtttaatac tcaataatct
      301 aaaaaattta gaaaaacaat acaatagcag ttattaggag gcgtttccca tggatttaaa
      361 aaactttata aggaatattg atgatttccc aaaaccaggc atagatttta aagatgtgac
      421 aactctattt aaagatggag atgcatttaa atatgcagtg gattctatag ttgaagaatt
      481 gaaagataaa gatgtagatt tagtaatagg gccagaagca agaggatttt tgatgggtac
      541 acctgtagca tatgctttag gagtaggatt tgttccaatt agaaaacctg gaaagttacc
      601 aggtgaagtt gagagttatg agtatggatt agagtatggt acagatactt tagagataca
      661 taaggatgca attaaaaagg gtcaaaaagt agcaatagta gatgatttat tggctactgg
      721 tgggactatg gaagccgctg ccaagcttgt agaaaaatta ggtggagaag tagtttctat
      781 gcaatttttg attgagctaa aattcttaaa tgggagagaa aagttgtcta actatgatgt
      841 taattctctt ataaaatact agtactttta taatagttgg tataacacca actattttca
      901 tatatattaa aattttatac aaaataggtg gttatatgca tgataaagaa ttacaagaaa
      961 taattgaaaa aataaaagtt tatgcaccta atgcaaattt tgaaatggtt gaaaaagcct
     1021 ataatcttgc aaaaaaagcg catgaaggac aatatagaaa atctggggaa ccatacttta
     1081 ttcatccatt agcagttgca aatattttaa tagagatgga attagatatt gaaactataa
     1141 cagcaggttt acttcatgat gttgtagaag atacagaata tacctatgaa gacataaaaa
     1201 aagaatttgg caaagaagta gcggatttgg ttgatggagt aactaaactt ggtcaaataa
     1261 aatatcgttc gaaagaagaa actcaatctg aaaatttaag aaaaatgttt ttggcaatgg
     1321 cgaaggatat aagggttata cttataaaac ttgctgatag attgcacaac atgagaacat
     1381 taaaatacat gcctccagaa aaagcaaagt ataaagcaaa agagacactt gaaatttatg
     1441 gtggtatagc tcatagatta ggaatttcca agataaagtg ggaactagaa gacagagcat
     1501 taagatttat ggacccagaa gggtattatg atttagttag tagagtatca atgaagagaa
     1561 gtcaaagaga ggattatatt caaggaattg ttgaacttct taaagataag tttaaagatt
     1621 taagtatacc atgtgatgta tatggtagac caaagcattt ttacagtata tatagaaaga
     1681 tgcaaaaaaa acataagact tttgaagaga tttttgattt aacagcagta aggatattgg
     1741 ttgataatgt aaaggattgc tatgcagtac ttggtatagt tcatacatta tggagaccta
     1801 ttccaggaag atttaaagat tatatagcaa tgccaaaacc caatatgtat caatcattac
     1861 acaccactgt tgtaggtcct gatggagaac ctttagagat acaaataaga actcatgaga
     1921 tgcataatat agctgaaaat ggtatagcag cccattggaa atataaagaa ggtatctcta
     1981 gcaaagagtc aaaggttgaa gaaaaacttc aatggttgag acagatgatg gagtgggaaa
     2041 aagatttaaa agacccacaa gagtttatgg atgcacttaa agaagatgta tttaatagtc
     2101 aagtttatgt atttacgcca aaaggagacg taattgaatt accagcagaa tcaacaccta
     2161 tagattttgc ctatagagtt catacaaatg taggtaataa atgtgtagga gctaaaattg
     2221 atggaagaat agttcctatt gattataagc ttcaaaatgg taatattgtc gaggtactta
     2281 catcatctaa ttctaatgga ccaagtaggg attggcttaa tatagtgaag accccaaatg
     2341 caaaaagtag aattagacaa tttttcaaga aggaaagaag agaagaaaat gttgaccgtg
     2401 gaaatacaat attagaaaga gaatttaaga agcatgatat accacaaaaa gaccctcttg
     2461 ttgaaaaata tatggcatta gttgctaaga aatttaatca gccaagtgtt gaggacttaa
     2521 ttgccactgt aggttatggc ggtataatgt catctcaagt ggtatctaag gtaaaagacc
     2581 tatatttaaa agaggttaaa aaagctaaaa aggaacaaca gatacaagaa gaagatttaa
     2641 ataaacacaa tataagtgaa acagaataca gtaaaaagag aaagaaaaac gcatcacaag
     2701 gaattatagt aaaagggctt gacaatatct tagtaagatt tgctaagtgt tgtaatcctc
     2761 ttccaggaga tgaaattgta ggatatatta ctaagggtag gggagtagcc atacacagag
     2821 ctgattgccc aaatgcaaac aataatggtg atttcttcca aaatagattg gtggaggttt
     2881 catggagttc agcaggaaaa ggtaagtttg aagcggaaat tcaaataaaa gctattgaca
     2941 gaagaggaat tgttaatgaa ataactcata ttgttgcaat ggataaactt agtttaaacg
     3001 gaataaatgc aagaaaaggt aaggatagta ttgtaagtat aaacttactt gtagaggtta
     3061 atgacacaga ggaattaaaa attctaatga aaaaattaaa agcaattcct ggtgtagaaa
     3121 gtatttatag aatgacaaat tagagttaaa ataataggga ggaaactatg agagcagtag
     3181 tgcaaagagt atcttcatct aaagttacag tagatgaaaa cactataggt caaataaata
     3241 aggggttact agtattgtta ggagtgactc atgatgataa atcaagtgat gtagattata
     3301 tgattgataa aatattgaat ctgagaattt ttgaagatga aaatgacaag atgaatttat
     3361 ctctaatgga tataggtgga gagcttttag tagtatctca atttacttta tatggagatt
     3421 gcagaaaagg aagaagacct ggatttagta atgcatctag accagaactt gcaaataacc
     3481 tttatgaaga atttgtaaaa aaagcaaaag ataaaggtgt gacagttggt acaggtcaat
     3541 ttgcagctca tatgatggta gagttaacta atgatggtcc agtaacaata ctattagact
     3601 caagtaaatc tttttaaggg ggaaaacttt aatgattata aaaaaatttg tagatagtta
     3661 ttttggagta aatacttatg tattaggtga tgaaaagact aaaaaatgtg cagttataga
     3721 cccaggtgga agtttagtag agacattaag ttttgtaaaa gaaaatgaat taaatattga
     3781 atatattatc cttactcatg ggcatggaga ccatatagga tatgttaagg atattaaaga
     3841 aaaaactggt gctaaagtag ttgcacatgt tgatgaaaaa gaattattaa atgataaaaa
     3901 taaaaatcta agttatacaa tgagatgtgg agcgcaagag tttgatgcag atatctatgt
     3961 gagtgataaa gacaagcttg aacttggtga attaaaaatg actttcttgc atactccagg
     4021 acatactcaa ggatgtatgt gtataagagt tgaagatgaa atgttcacag gagatacttt
     4081 gtttgctgga agtattggta gaactgattt atatagtgga gactttaacc aaatggaaaa
     4141 atcacttaag aaattatgca aatatgaaga tgctgttaga gtatatcctg gacatggacc
     4201 aaccagtact ctaggggttg aaaagaagac aaatccttac atgtaatttt atattagtat
     4261 ttaaaaaagt ataaaatatt taataactat attagtgagg gggttcttct actgttaaat
     4321 agaaggagtt aagttgatgt tgtatgtttt tttaaaagga catgattata agtatgaagt
     4381 agcagaactt ataaaattgt ttaccagtga atttaagttt aaagactcta atgctaacaa
     4441 taatatagag tcaaagcatt taataaatag attgatttat gagaatggtg tattattctc
     4501 aagtactgaa tactatgaaa atggaaattt aaagtacgag tcaatacaga atatagaaaa
     4561 tatgaatctt gaatttttag atggatttga tagtagcaaa acgattgaag aatttcttaa
     4621 tagactagat agtgaaagta agaggaactt taaaaagctg tgtaaagaaa atatcaaaaa
     4681 aagcatgttt gtagtgctta aacaagtatt taattcttat gtaccatggg gaattttgac
     4741 tggtattaga ccagtcaaaa ttgtccacaa tttaatggat aaaaagctag atgataatag
     4801 cataagaacc attctaaaag ataattattt tataatagat aagaaaatag atttagctct
     4861 tgaaatagct aaaagagagc gattatttat gtatcctata gataaaaata aaatttctct
     4921 ttatgtaagt attccatttt gtccaactag atgtgtatat tgttcatttc cttcaaattc
     4981 attaaaacaa tttggacatt taaaaagaga atatgtttat aaattgattg aagaaattaa
     5041 ggggtttgcc aaagttatta aggagactaa gaaagaagtt gaaactctat atattggtgg
     5101 tggaactcca actacacttg atgaggaaga attagatttg cttataaatt cattgttcaa
     5161 tgaattagat ttaagtaaaa ttaaagaatt tactgtagaa gcaggtagac ctgacactat
     5221 tacagaacaa aaactaagag tgttaaaaaa acacaatgtt agtagaatta gtataaatcc
     5281 tcaaactatg aatgatgata ccttggtaaa gattggaaga gagcatagag tatctgattt
     5341 agtagattgc tttaatatgg ctcgtagaat gggatttgat aatataaata tggatataat
     5401 tttaggtctt atagatgaag aattaaatat ggtaaagaac acattggagg agataaaaaa
     5461 actttctcct gaaagcctta cagttcatac attagccata aagagagcat ccaacttaaa
     5521 tataaatatg gataggtata aagaacatct aacacaatat gaagaaatgg taaaaatgat
     5581 agatttatca atgaaatatg caaaagatat gggattaaat ccatattata tgtacagaca
     5641 aaaacatatg ttgggaaatt tagaaaatat aggttatgct aaagaaggat atgagtgcat
     5701 ttataatata cagattatgg aagaaaaaca aagtaactat gcattaggtg caggtgctat
     5761 atcgaaattt gtttatgtag atgaagatag aattgaaaga gtagaaaatg taaaaaatgt
     5821 tgaacaatac attgaaagag tagatgaaat gataaaaagg aagaaagaag aggtatataa
     5881 aaatgttgac taaagctcct agaggaacta aagacataac tccaaaagag gcttacaaat
     5941 ggcgttatgt agaaaataaa tttagagaaa tatgtgcttt atatggatat gaagaaatgg
     6001 taacacctat atttgaacat acagaacttt tcaagagaag tgttggagat actacagata
     6061 tagttcaaaa agaaatgtat tcttttaaag ataaaggtga tagagaaatt accttaaagc
     6121 cagaaggtac agcaggtgta gtaagagcat ttatagaaaa taaattatat gcagatactc
     6181 aaccaacaaa attattttat gtgacacctt gttttagata tgaaagacca caagctggta
     6241 gacaaagaca atttcaccaa tttggaatag aagcacttgg aagtgatact ccttcaatgg
     6301 atgctgaaat aatcgcacta gctgttcaat tttttaatga agttggatta aatgatttgg
     6361 tagtgagtat aaattcagtt ggctgtccag tttgtagaaa agagtacaat gctttattaa
     6421 aagagtatct tgactcaaaa gctgatatat tgtgtgatac ttgcaatgag agaagagaaa
     6481 aaaatccaat gagggttata gactgtaaaa atcctacatg taaagaaaat atcaaagata
     6541 taccatttat tgcagaccat ttgtgtgatg attgtaaatc tcactttgat aaacttcaag
     6601 aatacttaaa ggaaatgaat ataaactttg taattgataa aacaatagtt agaggcttag
     6661 attactatag aaaaactgct tttgaaatta tatctaatga tataggagct caaagtactg
     6721 tatgtggtgg aggaagatat gatggtcttg ttgaacaatt aggaggacca aaaggtataa
     6781 gtggaattgg gtttggacta ggaattgaaa gacttttact tacacttgaa ggtaatggaa
     6841 ttgaaataga aaaccctcaa tctacggata tatttatagt aactataggt gaagaagcaa
     6901 atacaagaag ctttaaatta ttaaaagact tgagacaaaa tcatataagt gcagataaag
     6961 accatataga gagaagtgtt aaagcacaat ttaaatattc agataaaata aattcaaaat
     7021 ttacaatagt gattggagat gatgagctta aaaatgacac tgcgacatta aaaaatatga
     7081 aaacatcaga gcaaactact gtaaaattaa gtacattagt tgaagaatta aagcaaaagc
     7141 tgtaaaataa gaaagtatga tgttttgtgt attagataaa tatatagcat taaaattaaa
     7201 taaaatggag gtagtatctt ggaaacttta aagggtttaa agagaactca ctactgtgga
     7261 gagttgagag aaaaaaacat aaatgaagaa gtcgtactta tgggatgggt acagaaaaaa
     7321 agaaatttag gtggtcttgt atttgttgat ttaagagata caagtggatt atgtcaaata
     7381 gtttttgata cagatgtaga taaagaagct tttgaaaaag ctgaaaaatt aggggctgaa
     7441 tttgtaatag ctgtaaaagg aaaagtatgc gaaagacaat ctaaaaaccc taatatgcca
     7501 actggtgata tagaaatatt tgcaactgaa cttagacttt taaacaagtc agaaactccg
     7561 cctatttata taaaagatga tgatgatgtt tctgaagcac taagattaaa atatagatac
     7621 ttagatttaa gaaaaccatc tatgcaaaga aatttaaaat taagacataa agtaatgaat
     7681 attacaagaa attacttatc aaacaataga ttttgcgaaa tagaaacacc atttttaata
     7741 gctccaacac cagaaggagc aagagattat ctagtaccaa gtagagttaa tccaggaaaa
     7801 ttttacgcat taccacaatc accacaatta tataaacaat tattgatggt aagtggtatg
     7861 gataggtatt tccaaatagt taaatgcttt agagatgaag atttaagagc agatagacaa
     7921 ccagagttta cacaaataga ctgtgaaatg tcttttgtcg agcaagaaga tgttatgagt
     7981 atgattgaag gtttgcttga agcgatattt aaagaagttt tagatgtaga attggcactt
     8041 ccacttccta agatgactta tgcagaagct atgtctaaat atggttcaga taaaccagat
     8101 actagatttg gatatgagtt aacagatata tctgatgttg tttgtaactg tggatttaaa
     8161 gtgtttgctg atgctacaca accaggaaaa agtgttagag gtataaacgt aaaaggcaaa
     8221 gctgacgact ttactagaaa acaaatatca tcactagaag aacatgctaa gacatataga
     8281 gcaaaaggtc ttgcttggat gaaagtagga caagaaggag taacttctcc aatagcaaaa
     8341 ttctttaatg aagaagaaat gaacgctata cttacaaaaa tgaatgctga agtaggagat
     8401 ttacttttat ttgtagctga taaaaactca atagtatttg atgctttagg acaagttaga
     8461 cttgaagtag caaataggct taatctattg gataaaaatg tatataactt actatgggta
     8521 actgagttcc cagtttttga agaagatgaa gaaacaggca cattttcagc aatgcatcac
     8581 ccatttacat cacctatgga tgaagattta gacaagttag aagaaggtga taagtcatct
     8641 ttaagagcga aagcttatga tattgtattg aatggttatg aaataggtgg aggaagtgtt
     8701 agaatatcaa attctgatat tcaatcaaga atgtttaaag cacttggttt cacagaggaa
     8761 agagctaatg aaaagtttgg ttatttatta gaagcattta aatatggtac tcctcctcat
     8821 gctggtctag cttttggtct tgatagactt gttatgttac ttgcaggaac tgataatata
     8881 agagaagtta ttgcattccc taaaaatcaa aatgctgttt gccctatgac aaatgctcct
     8941 acattggcgg aagatgagca attagaagaa ttaagtataa aagttgatat aaaagataat
     9001 gaataatatg tagttgcaag aaactaaaat gtaaaaagtc tggatataaa gtatatttat
     9061 attcagactt tttttattag aattatttta tattttttct ctatatattt tcataaatta
     9121 tccttgtatt ctcagccaat ataattaaaa tactatgttt aaaaatttta cataaacttg
     9181 attgttttag aaattacttc tgtaatgagt acacgatttt aattaaagaa tatataacaa
     9241 atttgaaata agtctaatat atgacttaat agattaaatt aaaataataa atttggacta
     9301 tttatctatt tataacttaa attatatcaa attgataaca aactagatat tatacttttt
     9361 tgtagaatag aattacaatt atataaagaa aaattctatt aatagtgaaa aaaaagtaga
     9421 aaaaagtaga aattattctt ttataaagaa agggagggat aatattgcaa gagagagaag
     9481 aattagtttt aaatccagtt ccaaaagagg aaagagttgg atggttagct ccattgttta
     9541 atatgcttgg ttctaacata gctatatctg aactaatggt tggaggaact ttaattttag
     9601 gaatgactct ttctaatatg attattacat caattatagg gaacttaata ttagttgcaa
     9661 tcattatgat acaaggttat ataggttaca aagaagggtt aaatacgtat gtattagcta
     9721 agggggcatt tggagagata ggaggaaagt atcttatatc tctattactt ggtataacta
     9781 gttttggatg gtttggtgta caagcagggg ttgcaggact atcagtacaa aagatattcc
     9841 caagtgtaaa cctaacatta gttacagtta tacttggatt atttatggtt gtttttgcac
     9901 tgtatggctt taaagctatg gctaaattta attacttagt tataccacca cttatgatat
     9961 taatggtgtg gggagttttt aaagcttttt ctacttatgg agttgaagct atttataatt
    10021 atactcctca aacaactatg agtatggttg aaggtcttaa tattgtagta ggtcttgtta
    10081 tagttggtgc aattatatca cctgaccagt taagatatac gagacgagta aaagatattt
    10141 ggattatcag ttttttaggt ctagggatta tttcattatt ccaacaagtt gcagctggtg
    10201 taatgtctat gggagcacct acttgggata taacagaagt attggctaat ttaggttttg
    10261 gatgggtagc gtttgttatt ttaatacttg catcttggtc aacaaacgta tcaaatgctt
    10321 attctggtgg attagcatta aaaactattt ttccaaatgt aaaaagaaat gttttgactt
    10381 tagttgcagg tctaataggt acaataatag cagctactgg tataatattt aaatttcagt
    10441 catttttatc tttcttggga atcgcagttc ctgcaatagc aggaattatg tggtgtgagt
    10501 attattttat acaaggaaga acttataagc atagagaagg aataaattgg attgcagtaa
    10561 taagctggtt aataggtttt gcagcgtcgt attatagttc aaaaataaat ttcttaatac
    10621 ctccgataaa tggaattgta gtgagtatgg ttatttatta tatattaatg aaatgttttg
    10681 gaatcaaaga taaataaagg gggcaattac atgggtcgta aggtgaaaat aggtataatt
    10741 caacaacaca gtgtacttgg agatgttaaa aaaaatatag aaaaagcagt agagatgata
    10801 gatgacttag gaaaacatgg ggcagatata atttgtttac cagaactatt tgctacagga
    10861 tataatttag agtctttagg tggagttaaa acattagaat taataagaga acataataag
    10921 tacatagagg aatcaatgtc agaagctgct aaaagaaata atgtatacct gatatctcca
    10981 tatggaacct tagaaaaagg gtctacacat gtttataatt cagcagttat atttgataga
    11041 aaaggtaaga ttatgggaga atactgtaaa aatcatctgt ggtcattaga agcagtatac
    11101 tttaaaggag gggaaaaagt agaggtatat gatgctgatt ttggtagatt tggtgtaatg
    11161 atatgttatg atgcaggttt tccagaagta tccagagaat tgacattaaa aggttctgaa
    11221 ataatattta taccatctgc atggagaata caagatgaag atatgtggga tttaaatgta
    11281 tctcaaagag ctttagagaa cactgtatat acagtaggtg taaatttagt aagtaatgac
    11341 tctaatttaa tactatttgg aaagagtaaa atatgcaatc ctagaggtac tgtaataact
    11401 caattagata catacaggga aaaatatgta ttatcagaga ttgatttaga tgaaataaaa
    11461 aagtataggg aagatatacc ctatttgaaa gacagaaaaa gtagctataa catataattc
    11521 tatactataa tacaagttca atataaaatg ataaaaagag gatgtatcaa tattaagatt
    11581 aaatttatta tattttgata tatcctcttt aatattatca actttgattt aaatattttt
    11641 aaaatagttg ataagaaagt ttttgaacaa caacacagct ttagattcat agttttcgct
    11701 aaaccatgaa agatttaaat atcttttaaa ttcaatatct ttgatatgta acaacttaat
    11761 ctcagggtct tgagataatc cccatgattt tccacatata aatccaactc cttgaagtga
    11821 ttttataaga gcataaattg tatatggtga atcagactca aatgctattt tgggtttgaa
    11881 attagctgat tcgcacagga tatcaattac ctctctataa ttctcaccct tcgttataac
    11941 tataaagttt tcatctgaaa cttcgcttag atatacttca tcctttaatg ataaaggatg
    12001 gtttattgaa actcctaata gtatttcttc gcatgttaaa gttatgctat tttctgagtt
    12061 tagctttgta aatgaagaag atatgtacaa atcaaaatca ctttttttat aagatgatgg
    12121 caaagtatgt gacacattaa aagtaatatt gggatatagt tttctgaaat tactcaaaag
    12181 tgatggtaac acattagccg ctgcaggaga taatatcttt acttctcctg aattttctaa
    12241 agaagtgtct tgtaactcct tttttgaact ttctactaaa tctaaaatgc tatctactct
    12301 ttttagaaac aatcttccat actcatttaa aacaatatat cttccttttc tgtcaaataa
    12361 ctgaactcca agttcttttt ctaaggaaga tatagttttg cttaaagctg gctgtgctat
    12421 atttaacttg tgtgatgctt ttgttatgtg ctccattcta gcaacagttt gaaaatactt
    12481 aagatgcaat aaatccatag tataaaatac acctctttta atatgacttg agttatatca
    12541 attttataat caactccaca ttatacatta taatataaag tttatataat caactacaat
    12601 atattaaaaa gttatttaaa ttgttattta tattttgtac ataatctaat taaaaaaagt
    12661 agctttaaat gattaactaa ggaaatgttg ttacaaactt tagttatata aaaaattcat
    12721 ttgatgagta aagtattaat gtcaagtaag aataaaagct tattagatag aaaaataata
    12781 ttttataata atgatgttac ttaaattatt atgttatact attttaaata ggaaacattt
    12841 agatactata attttagggg gagtcaagat gaaattaact aaatcaatat catcaatgct
    12901 atgcttagct ctagttttaa gcttatcacc agtagcatat gcaaatgaaa taagtagaac
    12961 aacagtggag aatactttag taggtcagga cagataccaa actgctgttg aagtaagcaa
    13021 aaaaggatgg agtagtgcga atgaggctgt agttgtaaat agtgaatcta ttgtagatgc
    13081 attagctgta acaccatttg ctaaattaaa aaatgctcct atattattga cagaaaaaaa
    13141 taatctaaat tcacaaactg agaaagaatt aaaaagatta ggagttaaaa aagtttatat
    13201 aattggtcta tctagttctg tatcaaatga tgtgcaaaaa cagttagaat ctaataattt
    13261 aactactgat agagttggtg gtagtgatag ataccaaaca gctttgagta tagctaaaaa
    13321 aatagaagga ttaaaagata tatcagaaat agctgtagta aatgggtaca caggtcttgc
    13381 agatgcagtt agtatagctt ctgtagctgc tactaatgga atggttatac ttccagtatc
    13441 tgatgcatct ggtatatcta gctttaaaga ttttatagaa tctaagaata taaataaatc
    13501 atacataata ggctctacta atgctgtatc agataaaata aaacaaagtt tacctaattc
    13561 agaaagaata ggtggagcag atagaaatga aactaatggt aaagtaatag aaaagtttta
    13621 tacatctaat aaattaaaca atgtatttgt ggctaaggat ggaatgaaaa atcaaaatca
    13681 acttatagac gctctagcag taggagtact ggcatcaaaa gaaaattctc ctgtactaat
    13741 cgtaggagaa caattaagtg caacacaaaa gagtgtattt ttgaataaaa atgctacctc
    13801 tataactcaa gttggtagtg gaggaaatga aaatgcattt aaagaattaa aagatttaca
    13861 ggcaaatagt aacaataaga gagtaatgta tgtaactaat acagataagg taaatataag
    13921 aaatgatgct acaatagaag cttctgttat aggatatcta aataatggtg atgaagtaga
    13981 agttttagat gtgttaaaaa ctggatgggt aaaaataaaa tacaatgaag gtattggata
    14041 tgttagtggg tcttatttga caaataataa gcctgataat agtaatgaaa atataaaaat
    14101 aaagtatgta aaagaaaaag atggtctaaa tgttaggaaa ggaccaagta cagaagatga
    14161 aaaaataggt cacctttcat atggaagcaa agtagaaaca atagaaatat ttgctactgg
    14221 atgggtaaaa ataaagtata atggtggata tggttatgta agtaatgatt acttatctga
    14281 tacaccagtt atttaattat aagtaacaat aaaaaactgc tttaatttgt aaagcagttt
    14341 ttttataaaa atttaagtct aataactatt tctattttga taccttaatc ctgggattta
    14401 ttattatgga tattatcaac atacaaacac tccaaatcca gtgtctaatt ccaaacttag
    14461 atattaataa attcagtcct gctatggtaa ataagcagta tttaattaca attaaaagtg
    14521 tatttataga aatccccttc atagaaagta tcctaaatcc aataatagac attctaagtc
    14581 ctataaatgc caataatata cctgcaatat cctgaattaa gtaatagtat tcaaatttca
    14641 tattcaatca actcttctct gagcagtatc aaatatttct aactcttcaa tttcatcatc
    14701 tgtgtatgta agcatagtaa aaccagttac accataaata aatgaaatta taggtgtaat
    14761 ccaacttaag aaacaatatg gaataaattc tagtgggctt acaccaagag tctgagcaca
    14821 gaaaacagca tttgaattcc atggtattaa tggaccacca agtgtagctg tatcttctat
    14881 aattctagat agattttgtg gttttaattt aaactctttg tatattggag acattagagt
    14941 tccgacaaat acagaagtaa acatcattga tgaactaaat gcatttgcaa agtagcttac
    15001 tataaaagta gacccaacca atttagttct tgattttcct attttagtta taagaggttg
    15061 aagcagtact tctaagaatc cagttttttg gagaataccg gcaataacga aaactacaaa
    15121 tactagtaaa acggtttcag ccatactcat tattccacca cgatttaata gtttgtcggc
    15181 atagacaaat ccagtatcta tagtaaatcc tgatagcatg taatttaaaa tatcacctat
    15241 tttttctcct tcttgaaata ctgctataat aagtcccatg atagctgaac ttagtataga
    15301 tataatgggt ggcttttgta gcaataaaag taataataaa aatattattg gaatcattgc
    15361 tactaaacca atatgaaagt ttgaatttaa tacatccttg atttgattta tttgaacata
    15421 gtctatagta ttatttgaat acttaaatcc aattactgta tacagaacta tacatattac
    15481 gtatgcaggt cctgtggtat aaaacatatg cttcatatgt gtaattacat ctgtcttaca
    15541 aactgctgca gctaagtttg tagaatctga tagaggtgac atcttatctc caaagaaagc
    15601 accagaaatg actgcaccag ctgttaaacc aagaggaata cccatactag taccaatact
    15661 catcattgct ataccagaag ttccaagagt cccccaagat gtaccagtag caacagaagt
    15721 taatgaacat acgattaatg ttgtaagcag aaaatattta ggggttatta ttgttagtcc
    15781 actatatact actgttggta ttgtaccagc agctatccaa gctgctatta gtacaccaac
    15841 agaaagtata attaagtttg attgaaaggc atctttacca acttcaaatc caaaagcttc
    15901 tatttcatca ttagtataac caagtttcgt ggctgcagga actactataa gccatgaaag
    15961 taaaaacatt gtagttatag aagcattaaa tacaactata ccaactgatg ttagagctat
    16021 tattgcaagc attactaaca aggaatatct aaatgaaggt atttttcttt ctgtattcat
    16081 tttcatagtg caaattctcc ttaaatattt ttatttaatt gcatttttta ttcttataag
    16141 agcttcttca agcatacttc ttggacaagc taagtttatt ctttgatacc cacttcctcc
    16201 aatgccaaag ctgtttcctt gatttagagc aactttacct ttttgtacaa gtacactttc
    16261 tagttcctca tcacttaatc caagtgcact aaagtctacc cataataaat aagtaccttc
    16321 tggttttcta actttaagtt ttggcatatt ttcatttata tattttatag caaaatctat
    16381 attagattct aaatattcta aaaatgattc tagccaagat tcaccattat tatatgaagc
    16441 ttcagttgca actaagctaa agcaattatt tctctttata tcaattctgg taaaagcatc
    16501 atctaacaat ttatagtctt tttcatcagg tagtattaca tatgatgatt gaagaccagc
    16561 tatattaaaa gttttagttg gagccataca agtaattgta tttttttcaa attctttaga
    16621 aatagatgcc ataggaatat gcttatgttt ttttaatata atgtcactat gtatttcatc
    16681 agatatgatt tttacattat gcttaagaca aatatctcct agttttttta attcatcttt
    16741 tgtccatact ctaccaactg gattatgagg attacaaagt atgaataatt ttacatcttt
    16801 aatcttgttt tcaatgtctt cataatccat tatatagttt ccattttcta gtttctgtaa
    16861 tgggcttata attagttctc tattgttatt ttttacaaca ctattaaaag ggctgtaaac
    16921 tggttcttgt atcattattt tatcatttgc ttttgtgagt tcatttatta aaagacttat
    16981 agctgggata acacctggac tataaattaa ccactcactt tttattttcc agttatgtct
    17041 cctatctagc cagtttacta tagattcatt gtaagaatct ggtcttgttg tgtagccata
    17101 aatctcttgt tctagtctat tttttaagga atcaattata caaggtgcag ccttaaaatc
    17161 catgtcagcc acccacatag gaagtaaatc attagttcca tacttttttt ccatttctga
    17221 ccatttagat gaaaaattat tgcttctatc aacaatctca ttaaaattat aattcattgt
    17281 aaatacctcc agtaatttta attttatgtt aataatatta agcaaacaac gtgccaatat
    17341 tgataataaa aaatatttat tcacaagtta atatttaata ttaagtaatt tcagagcata
    17401 gataagataa aaatattaat tatacttaaa actttgtgga aaaggttatt tctaataaat
    17461 ttttaaatat atgaaaatgt aattaaatag gttaaatagg tttaactgtt taacttgtta
    17521 gacctattta tattttttag aaattctact ccttttgatg ttataattga acctccacga
    17581 ccaacaagta tgtctacaag cccaaagtct tgtagttcta aaagcatatt tctaatctgt
    17641 tgttctgtca aaaatctatt ttcctctaaa gctattttac ataagctttt tctacctatt
    17701 ctttctttta aatcataagc cttttttaaa ttatccaata taaatatgta ttcatctagt
    17761 gaccttttaa aattatagtc gtatttcatg atgtttttgt ctttgccaat gttgaattga
    17821 tactttttta tattatcact tactttatta caaacggttc tactataatt tgaatctata
    17881 gtgtccaata tatattcagg taaatcacat atttctataa tatcttttcc caagtaacag
    17941 aagtattctc ctaagtttct taattctctt acatttcctt cccatctata cattttaaag
    18001 atttctttta cttcctcact tagaataaaa ttacatggaa tatcatattt taatgaacca
    18061 aaaatttcaa atatatcagc agctctttcc ctaagtggat gtattttaag gggaagaaca
    18121 ttcaacctaa aatataaatc tcttctaaac atatttttag atacaagttc ttttaaattt
    18181 ctattagtag ctgcaattat tcttacatca atatcaatga cattattaga accaatacgt
    18241 cgtacttgtt tttcttgtat gactctaagt aatttagatt gagaatttaa atccatttct
    18301 ccaatttcgt ctaaaaatat agttccattg ttggctaatt caaataaacc aattttccca
    18361 ccttttttag caccagtaaa agccccttca tcatagccaa ataattcact ttgcagtaga
    18421 ttttcttgaa aagtactaca atttactgcg acaaagggac catcttttct cctagaagca
    18481 ctatgaatgg attgagcaaa taattcttta ccagtaccac tttcaccaat tataaggata
    18541 gatgaatttg attgagccat tttattagca attttttttg tattaatagt ttgtatacta
    18601 gaacctaata tatcatcaaa tgtgtattta ctgatattac cagagtttac tagttgtgct
    18661 cttaatttag cttgcttttt ttctaattga gaaaactttg tcatctttag tataaatcca
    18721 tcaaatacat ttaattgtat atgtttaatc tctaaattta tatcaatatg atttatttta
    18781 atcaatttct caaaaaatgg agttttattg gtcaatatgt tttgaaaatt gatgtctttt
    18841 atacaatcac caacaaatga gtcaatcatt tcatttgcat ttatagacaa aatttttcta
    18901 gctatatgat tatagaattg aattattcca tcattatttg tgcatataat tccatcatca
    18961 attatagata gtaacaaatc aaattgtctt tcaaacttat ttgcatctaa caataactca
    19021 cctgtactat aacttgtagg aattattttt tctacgtatt tttttattag gtcatctttt
    19081 attagatgtt ttaatttggt ttttgtagca atttcaacaa tacaacttaa atctattatt
    19141 ctgtatttta aatctacaac ttcacaattt ttaaacttag gcaggatttt ttcgccagga
    19201 gttaggatta cagcatctgt gggagtaaac tcaatctctg gatagcaggg caggatattt
    19261 atattgtcta ttcctaatct atgtattaaa gaaattgttt ctattgccat atctttactt
    19321 aagttatata cataaataat tttatctttt tctaatttgg atatcatatc aatactgttc
    19381 ttttcaaatg tcaacttggg aacaatgatt tgagcatcat agtgagataa ttttactatt
    19441 tcatcgtatt ttaaataagc agataggagt ataagttttt ctttaaaaaa ctttaaattt
    19501 ccctcttcaa atgagtataa atttatcttt agattatcac ctaagaattg ccttatttga
    19561 ttttcataaa tttctccagc gtcttttttt aaacttacaa tagctaattc catttgtttc
    19621 attttaacct ctcatacaaa tttgacttat tttagattac ataataatta aattagatta
    19681 aataatatta tttaattatt attataaatt tattatagac tacacattga agtaaaagaa
    19741 gtagctaaaa ttattccttt tatttttgtt ttttgtatct atttggtata atagtattag
    19801 agcgtataat aatttaggca aaatttttta aggaagacta gaggagaata taaaatgaaa
    19861 aaatgtcttt actgtaatag ggatataaag tcaccaaata ataaattagc tataaaatat
    19921 gatgacaata tgaatatata tccatgtcgt gcagaatgta aggaaaaaat tgaagaatat
    19981 atagcaaaaa aacctacata taagtttata aatatattag aggttctttt aggtgtgggc
    20041 ggaatattct gctttttgag taaatggact attgcagccc aagttgtact tggaattgat
    20101 gcactggtta tatttttatt cccattggca ggatttaaga tgaatggaaa gttaagtatg
    20161 gaacaaacta agaatcaaag taggtctatt gcaatatcaa ttttggcatt tatagtaata
    20221 ataaccgtat tatattttaa acaagttggt aagtagagca taattgtata agacatcatt
    20281 agagtatgag gtgagtttat ggaagaaaga aaattagcac caaacaatga gagggaagct
    20341 ctcataaaaa gattaaaaag aatagaaggt caagttaagg ggatacaaaa gatggtggaa
    20401 caagaacgtt attgtgttga tatattggtt caaatatctg ctataaggtc tgctataaat
    20461 agagtaggca caattatctt agagaatcat ataaaaggat gtgtagcaga aagcattaaa
    20521 catgatgatg ctgataaaac tgaagagatg ataaaagagc ttatggatac tataaataaa
    20581 tttacaaaat aaggaggcgg ttagttatga atcaacaagg atttattatg gaaattgttg
    20641 atgatagaac tgcaaagtta aaaatgcaaa gacattcagc ttgtgctgct tgtggaaaat
    20701 gtgctactac aaattcagca gagtcaaaag aaatattagt agaagtagac aacacaattg
    20761 gtgcaaaagt tggtgaccat gtagaagtga gtatggataa tatgaatgta ttaaaagcag
    20821 ctgtaatggc ttatataata cctcttatag cacttcttgt aggaacaata gggacttatt
    20881 atatattggg ggcaattgga atgacaactg gagttgaagc cataagtgga ttagttggaa
    20941 tttgtcttac aataatctgt tatttatact taaagaagaa tgacaaaaaa tttagagaca
    21001 gtagagaatt tataccagta ataacaagaa tattaattga cctttaagat aattataatt
    21061 aaaaaatagc aatgtgcttt ttaaataaat actcaatacg tatttgtaga gtttaagtac
    21121 attgctattt ttatattttt attttgattt tctgtaagga ataaagtttt ttctgatttt
    21181 caaaagattc aatgttctta caaaagaagt tacaactaat acaccaagtg ctattgcagc
    21241 acttagaaat agcgcatctg taccttttga catagccaaa gaataatttt tctgaatcaa
    21301 agacaacatt gtattatata gcccaattcc tggtattaga ggaagaatac ctggaattac
    21361 gaacacaata gctggttgct ttagttttct tgctaaagtc tcactacacg ctgagaccaa
    21421 agcagcagca ataaaacctg caaaaactgc atttgtagta aaattaaata aataaacata
    21481 tacaatccaa ccaattccgc cagttactcc agctggaaga agtagatata gtggagcatt
    21541 aaagaaaaca gcaaatccag cagttgcaac acctgaaaat aaaaaatgca aaatcataga
    21601 catatcagtc attagaaagg acctcctatt ctataccatg tatcaaggac aaaaccaact
    21661 ccagaagcaa ttgcaataat aatcatagca gcatcaaaag cacgagaaat tcctgatatt
    21721 aaattccctg atattaaatc tctaatagct tttataaaag caactcctgg gagtagtggc
    21781 attatagcgc cgactataag cattttaggg tcatctaaaa tgcctatttc aactagtaga
    21841 actcctgaaa gtgcaattaa aaaagaagat actaaacagg aaaatgcaga tattgcactt
    21901 atcttcatcg ttttatcata agtaattgca gcaaagattg ctattataaa cgtaagtata
    21961 aaatttaata cataattccc tccaagcaaa caagcaaaac aagcagatgc aattcctgta
    22021 caagagtaaa ataaccataa aggataaggt tttacatctt ggatttcata aagacgtgca
    22081 atggcattat caatatcata ctctttgtca ctaacaaatt ctcgagaaaa actgtttaat
    22141 aaagaaatct tgtttaagtt tatacctctt gattgaatcg ttttcataaa cgaaaagcca
    22201 tcaaatttct catctgaaat aattattact gtaggagttg taaaaatatt tacatgataa
    22261 aaccctcttg acctgcatat tctaaggaca ctgtcttcaa ccctagatgt ttcagctcca
    22321 ttttttatca tcagttcccc tacaaataga gctagtttta gaatattttt cttatattca
    22381 agttcctcat tttgattcat aaatacacct ccaaattaaa aaacgtaata ttgaaattat
    22441 atcaaatttt taagtaatat tccttaatat ctatataaat atattatctt ttttttgttt
    22501 caaaatagtc acatttaaat tatatccaat taaaagaatt agacaatatt atttattata
    22561 ttttaactta aaaataaaga aagtaactat gttaaatatg gaaaaatggc aaaacacttg
    22621 actattaatt ggtaaaggac tataataata aattatcata ttgataaaaa cgaaaattat
    22681 gaaatgtctg taaaaataaa atttaaataa taactaggag gagtaatatg tttgagaact
    22741 tgaaaaaagc ggatcctgag atttatgaaa gtatgaaaag agagttaaaa agacaacaaa
    22801 gaaatattga attaatagca tctgaaaaca tagtatcagt tccagtaatg gaaactatgg
    22861 gaagtcatct tacaaataag tatgcagaag gttactcagg caaaagatat tatggtggat
    22921 gtgaatacat agatgaagtt gaaactcttg caatagatag aatcaaaaaa atatttggag
    22981 cagaacatgc taatgttcaa cctcactctg gagcaaatgc aaatattggt gtatattttg
    23041 ctatgttaaa accaggtgat gttgttatgg gaatgaattt atctcaaggt ggacatttaa
    23101 ctcatggtgc tccagttaat atttctggtc aatattataa attttatgaa tatggtatag
    23161 ataaagacag tggacttata gattttgatg aagttagaaa gatagcacat gaagtaaaac
    23221 ctaaaatgat agttgcagga gcaagtgcat atcctagaga aatagatttt aaaaagttta
    23281 gagagatagc tgatgaggtt ggagcattgc ttatggtaga tatggctcat atagcaggtc
    23341 ttgtggctgc tggtattcac caaaatccat gtgaagtggc tgattttgtt acaacaacaa
    23401 ctcacaaaac tttaagagga cctcgtgggg gtgttattct ttgtaaagaa aaatatgcta
    23461 aagacataga caaagcaata ttccctggta ttcaaggtgg acctcttgaa catataattg
    23521 cttctaaggc agtttgcttt aaagaagctt taagtgatga gtttaaagaa tatcaggtac
    23581 aagttgctaa aaatgcaaaa gccttagctg aagaacttat taaaagagat tttaaattga
    23641 tttctggagg tacagataat catttaatac tattagattt gattaataaa aatataactg
    23701 gtaaagcagc agaaaagaga ttggatgatg cttatataac agctaataaa aatacaattc
    23761 catttgatcc aaatggtgct cttgttacaa gtggaataag acttggtact ccagctgtaa
    23821 ctactagagg tatgaaagaa gaagatatgg caataatcgc tgaggctata gacctatgtt
    23881 taacttatga tgaagaatct aaggctagaa cattggttgt tggtttaact gaaaaatatc
    23941 ctttatatga agaatataat attatggatt aattataaag aacaactaaa taaaatacat
    24001 ttaaataatt acaatgattg tgtatgtatt tgaatatatt tagatataaa aaaggtgtct
    24061 caaaatgaac tttttagttt atgaggcact tttttgtatg gaatcgaaca tatttgtgtt
    24121 atttaaataa taaaaaatat ggttatctct atttttagat ggtctctttg tattttttaa
    24181 ttaaaacttt aagatttctt aagaattata ctaggttttt ctaaagattt aaagtctata
    24241 cttataagta tagtaatcat atgaaaagaa aagctttaac gaggagggaa tcttatgaag
    24301 gtattaattc ttacaggaaa atttggtatg ggtcattact cagcatcaaa ttctttaagt
    24361 gaagatataa aagcaaaatt tgataattca gaaataacaa ttaaagatat atttgaatac
    24421 attatgccta actattcaga taaaatgtac aagacttttt ctattcttgt aaatcgggga
    24481 agtagtcttt ataatttgtt ttataaatgt gctgaaaatg gaaagaaaga tataaaattt
    24541 actttttcag attatttttt aaataaatta gatacattac ttcatgaagt acaaccaaca
    24601 gtagttattt caacttttcc attttgttct caattagtat caagatataa ggaaaaatat
    24661 aactcaaatc ttcctttaat aacatgcata actgatataa gtagtcactc agaatggatt
    24721 agtaaaaata cagactgtta tttagtagct agtaaatcta ctaaggaaga acttgttttt
    24781 aaaggaatag atgaatctaa gattaaagtt aatggtattc cagtaaaaaa agaatttaaa
    24841 agaatagaac atgtaaatca ttcaactaaa aaaaatattc ttataatggg tggaggatta
    24901 ggattactgc ctaaatcaga acaattttac aaagaattaa atagtttaga aggagtaaaa
    24961 actactgtaa taactggtaa taataaaaaa atgtactata aactatatgg tagatatgaa
    25021 aatatagagg tagtaggtta caccaatgaa gtatataaat atatgaaaga ttctgacctt
    25081 attatttcta aaccaggtgg aataacacta tttgaaacaa tttattcaga acttccaata
    25141 ttggcattta atccattttt acaacaagag atagataatg ctagttttat attaaataat
    25201 gaaattggga gaatacttgg taaaaataaa aaatactatg tagatgagat aaaagattta
    25261 atttatgatg atgctactct taaagaaatg agcagtaaca tgaaagaatt aaaaaaacaa
    25321 tttgacaata ataccttaga gaatatttta ttttcacttg atgaacaagg agcgtgtaga
    25381 gaatgtatgt agtaggttta attatcgttg ttgcattgat ttttttagtc cattcaatta
    25441 tacctacata ctataataaa ctattaaata aagaggtatt aaaaaatatg gctggagaaa
    25501 atgaaattgc acttacattt gatgatggtc cagataagag atatacagaa aaattactag
    25561 atgtgttaaa agaaaatgat atacaagcta tgttttttgt agtagcaaag aatgctgaaa
    25621 aagagcctga aattataaaa agaatgttga gagaaaatca tatagttggt ttacattcat
    25681 tggaacacag aaatgcatgg ctttatagtt atagttatgt taaaaaagat tttattgaaa
    25741 gtacaaatat aatgaaaaat ttaggtgtag atgtaaacta ctatcgtcca ccatggggtc
    25801 atactaatat attttcaaat tcatttgtta aaaaatataa tctaaaaatg actttgtggg
    25861 atgtaatggc agaagattgg gagaaagata gtacagtaga cattattata aacaagttaa
    25921 tgagtagaac aaaagaaaat tctataattt gtttacatga tgcaggtgaa aattcaggtg
    25981 gtgcagtagg tgcacctgag cgtactattg aagctttaaa gatagctatt ccaaaactta
    26041 aagccagtgg tttaaagttc gtaacaccag aaaggatgta gttatgataa gtaaaactga
    26101 aaagaaaaat aaatgcctag gtagtgtagc ttttttagtt ttactaatgg gcataacagg
    26161 atattttgtt tttagagggc agtcagttga atcattgata aaatcattaa aaggtgcaag
    26221 ccctatgttt atactaattg gatttgcaat gatgtttata tatgttgcct gtgaaggtat
    26281 aaatatatac ttaggtatga aagctttaaa ccaaaaaact actcttttaa aatgtatggg
    26341 atatgctttt attggatttt attttagttc tattacgcct tcagcatcag gtggtcaacc
    26401 agcacaagtt tattatatga aaaaagacga tataaatata tcatattctt cacttatact
    26461 attagtcatt gttgttattc accaagtagt tatactggca tatagtggaa taatgttcat
    26521 tatggaacgt gagtttatat taaataatgt aagtggtatg aacattttac taatatatgg
    26581 tgtaataaca aatgttgctc ttgtcattgg agtaattgca attatatttt caaaaaaact
    26641 tgtaaacaat tttataatat caataacaaa tttattaggt aagttaagaa taataaaaga
    26701 tgttgaaagt tcaagaaaag ttataatatc tcaaattgaa gagtatgtta aaggtgctca
    26761 atatataaag cagaatccaa aacttgtagt ccaaatttta gtcataacta ttgtacaaat
    26821 aacagctatg tttttagttc ctttctttgt atacaaagct tttcatctat caacatatac
    26881 tgtatttgag atattagcga tacaatcctt gcttaatatt gctgtttctt cattaccatt
    26941 gccaggggct gttggagctt ctgaaaatag ttttatgaca ttatttaaga tatttttccc
    27001 tggtcactta ttagttccgg caatgttact tagtagggga ataagttttt atgcatttgt
    27061 agctataagt ggattgatat gtatagtagt acatgtaaaa tcttctgaaa aagtaaaagc
    27121 agtgaaaaaa gttgcttatg taagataata ttatatagat tatttttatt aatatgtcaa
    27181 tttgtgtgta cattttaatt aagttttaaa catgtttaca ggagtataaa ctattaaaac
    27241 gtgtgacatt gctaagcttt tgctaataaa gtataatatt ttatatagtt tgaaatattt
    27301 aagaaatata gcaaaaaaag gagcttggtg atgaataaag agattccaaa aaaaatttta
    27361 gtagctgatg ataatcctga gattagggaa atcgtagaaa tacttttaac tggtgaaggt
    27421 tatgaagtta ttatggcaac gaatggagaa gaagctgtaa acttagtaga tagtacgatt
    27481 gatttgatta ttttggatgt tgtaatgcct gtaaaaacag gatttgttgc atgtaatgaa
    27541 attagagaaa aaacgacagc accaatatta tttttaactg caaaaactca agactctgat
    27601 aaagttattg gattttcagc aggaggagat gactatttat caaagccttt ttcatattct
    27661 gaactaattt caagggttaa gtcgctactt agacgttatt atatatacca gggaaaggaa
    27721 aaggaaagag agccaaatat attaaaaaca aaagagttat gtgtcaattt agatactcaa
    27781 gatgttacac taaatggaga acctgtgttt ttgacaacta tagaatatag tatattaatt
    27841 cttatgttga aaaatagaaa aaaagttttt tcgtctgaaa atttatatga aactatttgg
    27901 gatgagcagt atttttatac ggcaaataat acaataatgg ttcatattag aaatttaaga
    27961 aaaaagttag aaaaagacca aaaaaatcca caatatataa aaaccgcatg gggaaagggg
    28021 tattatattg attaacaaaa ttaagccatc taagttggga gttatactat tgatatatgt
    28081 agtaatctca gctgcagtgg ctttcatttt aaatatacca ttttactctg ctgttgaatc
    28141 tatagtaaaa gatgtatcag aagggaaagt atattttaaa agagaagtaa atcaacttat
    28201 ggataatttt caaacttatg tgaataacaa tggtataaga gcagatgatg gagatgatat
    28261 agcaaaatgg aatagtgata attggtttgt ttctatgaag atatataaag gaaataaggt
    28321 tttttatgat acattatact atcctaatga agtacctgat aagagtgagg aaaatgagtt
    28381 ttatagcagt cccatggcta gaaactatga gatacaattt tctgatatta aagctaatat
    28441 agttgtagta gctttattta gatttagatt tcaaaatttt atagaagcaa tttctattac
    28501 atttatggca cttacattta ttataacatt tttactgttg tttagtagaa agataaagta
    28561 cctaaggaaa atagaaatgg gtataaagat agtagagagt ggcagtttaa agtatagaat
    28621 cccagtaaaa ggtaatgatg aattatcatc acttgcatct agtattaatg agatgagtca
    28681 tacattggag aatcaactta taaaagaaag ggagatgaag aataaaaata aagatatagt
    28741 aacttcgata tcacatgatg ttagaactcc attaacatca gtaatttgtt atttagattt
    28801 gatcaaagac cataaataca atagtcctga gcaattggaa caatacataa aaaatgtaag
    28861 gtctaaagca tatcaaataa aagatttaac agatgacctt tttacgcatt ttgttaattc
    28921 tggagaggaa gtttcaaaag ggtatgaaaa aataaatgct aatgaattta tttttcaatt
    28981 acttttagat agttcatata cattggagga gaaaggattt catataacaa tagaaaatga
    29041 actttataac caatatcata tttatgtaga tgttacacaa tttagaagga tatttgataa
    29101 tttatgttct aatataataa aatatgctag gaaagataag cctatagtat ttttaattaa
    29161 agtagaggat tctaaattac taattataca aaaaaataga gtaagtaatt tttctaagaa
    29221 tgtagaaagt tttggaattg gtataaagaa ttgtgagaaa atagttgcat atcataatgg
    29281 aagcataaat gtaaattcag atgataaaga atttgctatt gaaatatcta ttccagcaaa
    29341 agaatcataa aaatttaaat tatatattgt gtaattgcta ttatatattg tgtaattgct
    29401 tatgtaaaat tctatgaagc aggaagcatg tgactttagt tatgggatgt tcaatattgt
    29461 gtacctctag gcaagtaaca agtttactta caagaccata taatattatt atcttaaaaa
    29521 aaggggacat aataatatgg ttttgacagg agcacattat atttacattc tatttatggt
    29581 aattatatta attactatga ttatgaaaaa ggatacaata gtaccatgta tattaggtgt
    29641 tttttttatg ggattgttct ttgaaaaaaa catctttggt gctataacag cagtgtttaa
    29701 ctcttttatt atatctttaa atgagttggg tccaattatc ttgattatag ctattatggt
    29761 tgcattatct aaagccctag aagccaataa tgctatacag tatatggtga gaccattttc
    29821 gagagttata aaaaattcta atacagcatt ttttgtaact ggatttgtta tgttagttct
    29881 ttcttggttt ttctggccaa ctccagctgt tgccttagtt ggagctgtat ttttacctgt
    29941 tgctatgaga gctgggctac cagcaattgg tgttgcagta gcattaaatt tatttggtca
    30001 tggtcttgcc ttgtctacag actttgttat tcaaggagct ccaagtataa cagctggtgc
    30061 agcaggtgtt gcagtatcat atgttataaa tgatggtatg attttgtttt gggtgatggg
    30121 aatcgtaacc atatctatgg cattttatac attaaaaaga gatattaaca aaggaatgtt
    30181 tagagaagaa ctaaaaggtt ttgaaagtga agaagtagaa gagttcaatg gaaaatctaa
    30241 aatagcaact attttagtag ctctgggatt tcttgctgat ataattgtta tgtatatatt
    30301 tgacctaaaa ggaggagatg catcagctct tttgggtgga acagcagtat ttttaataat
    30361 aattataaat gttataaatt ttggcaaaga tagtcttgaa aatgtatgtg agaatataat
    30421 tgatggtttt gtgtttggga taaaaatatt tggagcaatt ataccaatag cagcattttt
    30481 ctatatgggt gaagttgcgc ccttgacagg tgtatttgga aaagtattag caccaggttc
    30541 acaaggttta ttatcagata taggcatagc tctttctcaa acaataccac ttaataaatt
    30601 tgccgtatca ggtatagaaa ctgtagtggg ggcaataact gggcttgatg gttctggatt
    30661 ttctgggatt tcattagttg gttcacttgc ctctgtattt ggaactgcta taaatgcaag
    30721 tgttggtgca ttagctgcat taggacaaat cagtggtata tgggtaggtg gaggctgtct
    30781 agttccttgg ggtcttattt cagcagcggc aatttgtggg gttagtccaa tagagttggc
    30841 aaagcgaaac tttgtcccag ttataaccgg attggttgta acaaccatag tagcaatgtt
    30901 tattatttaa taaatcgaat aaatctttaa ttgtacaaat taaaaataga tgtgtagcta
    30961 taatttatta taattgtttt actaatttta agccatacat ctatttttat tgatattttt
    31021 attagtattc tttaataaaa taatattttt cattatacta tacataacat ctaaatataa
    31081 catctaaaat actgttatct atgtttaata tcttgccaaa ttttttgatg ttttattacc
    31141 ctgtcccacc aacatatata agctatcgca agttttttac ctatactatc accaaacaaa
    31201 aattttaatt ttaatttatt attttataat ctatattata ttaactatat tagtttgtag
    31261 actttagatg taattctaaa tattaatact ccaagagttc aaattcatat cctagttttc
    31321 ttgcatttga tataaaatca tttaaaattt ctgtactagt tttggaaact gcatgtagta
    31381 acaagataga accatcatgt agattatcca ttattttttg cgttgcttct tcatgtgatg
    31441 gttgtttatc agtatcccag tctctgtatg caaaactcca aaatacagtc ttgtatccta
    31501 aatttttagt cattgccaag cttttttctg agtattttcc cataggaggt ctaaaaaatt
    31561 taaccatatc tttcccagtt acatctttat ataacttttc aacatcatat aattcgtcat
    31621 taaagttttt tagattgctt gtttttgttg gcatagatgg atgggtttta ctatgatttc
    31681 caactatatg tccctcagaa accattcttt ttactaaatc tttattttct tttatatatg
    31741 gagctgtaac aaaaaaaact gctttaacct ggttttgttt tagagtgtcg agtatctttg
    31801 tagtgtatcc attttcataa ccttcatcaa atgtaagata tagtgttttt tgaccatctt
    31861 ttgtaggtcc attgtatagg gcatcatagt cagaaaattt aaattcaatg tccgtattta
    31921 cttctggtgt tttatgctta ttgttaggta tataaaacca atcaagagta gtgttatcta
    31981 aagaagatac atctaattta ttattatcat tattagtagt ggcttttgat ggtttatctg
    32041 ttccgtctga aactattaca ttggaagtat ccttaccacc atctattttt tccttatctt
    32101 cttgtaactg tgtttcctta ttttgatttt cgttttgatt gttttgtgaa tttgaacatc
    32161 caacaataaa aaaaatagaa atagaaagta gtgttaaata atgcaatact tttctaaaca
    32221 ttttgaagct cctttctaaa ttaataataa ttgcatatag tattattata attggaaatt
    32281 actattaata atagttatat ctttaaaaat aaagtcacaa tagtatgaca aagggtgaat
    32341 aatcaataaa tttaaaaaaa aatgaaaaaa atagttgacc atattatgag aagatgttat
    32401 tataataaat gtgctcaaca agaagtaaca aaataacaag cacaaagaag ttaattgaat
//
LOCUS       DAELXX010000034        32305 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.034, whole genome shotgun sequence.
ACCESSION   DAELXX010000034 DAELXX010000000
VERSION     DAELXX010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32305
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.034"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            64..381
                     /gene="flgB"
                     /locus_tag="KPH29_001535"
     CDS             64..381
                     /gene="flgB"
                     /locus_tag="KPH29_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HBG1467154.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYVTFEETL
                     NDAMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMATLQYNALISFANGKIAMTKSII
                     SGR"
     gene            389..793
                     /gene="flgC"
                     /locus_tag="KPH29_001536"
     CDS             389..793
                     /gene="flgC"
                     /locus_tag="KPH29_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HBG1467155.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKEVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            803..1120
                     /gene="fliE"
                     /locus_tag="KPH29_001537"
     CDS             803..1120
                     /gene="fliE"
                     /locus_tag="KPH29_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBG1467156.1"
                     /translation="MPITQINNLMQGAKLDNLIGETKPLTLNNKEEKKNFEDVIIDTI
                     DKVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNK
                     VQI"
     gene            1145..2695
                     /gene="fliF"
                     /locus_tag="KPH29_001538"
     CDS             1145..2695
                     /gene="fliF"
                     /locus_tag="KPH29_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HBG1467157.1"
                     /translation="MIASFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDVQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTGGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAIPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDEDVDENNQPTNSLINVDKNKASEQALNKQLEKSILEMLEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSATNNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKAEAKQELEEIKKEGFKKNIIKAVIGVVAVVAIGAIGYL
                     IYKKRSEREDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            2708..3778
                     /gene="fliG"
                     /locus_tag="KPH29_001539"
     CDS             2708..3778
                     /gene="fliG"
                     /locus_tag="KPH29_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBG1467158.1"
                     /translation="MSTEITNVNAFDLSDINESDKKEKIEGEGLKKAAVLLMTLGPNV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNAKANKLLEGISINTSTKPFKHIIKLDVKKILEILRDESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAMRISSMGSISPEVIKVIESSLEDKLHSISGDDFKDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQDPALAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDEISDAIYRNQSSRAADALKEEMEMVGVIKVTQVEAAQQKIANI
                     VRKLEREGAITIVRNFGGDFVE"
     gene            3771..4502
                     /locus_tag="KPH29_001540"
     CDS             3771..4502
                     /locus_tag="KPH29_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBG1467159.1"
                     /translation="MNNKIIKSKKASYKGVLDLTIIDKVRVGESNVNKENDCRFRDEV
                     IKEAIESAEIKKNEILEEAKMNAQNIEKQAYEKGYSQGQKNGYEDGYKESYDEYTQKA
                     KDEADFIKNQADLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESMSDL
                     LKQIIQDYEGSSSFIVRCNSFYKDNIEKEFELFKEETSLKSSVFVIADDSIDKGNAII
                     QKENGRIIVGIDCAMEKLKEELLGE"
     gene            4506..5825
                     /gene="fliI"
                     /locus_tag="KPH29_001541"
     CDS             4506..5825
                     /gene="fliI"
                     /locus_tag="KPH29_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HBG1467160.1"
                     /translation="MYKTDISIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVCATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGIAIG
                     EPPAQKGYTPSVFAILPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRRIFE"
     gene            5857..6303
                     /gene="fliJ"
                     /locus_tag="KPH29_001542"
     CDS             5857..6303
                     /gene="fliJ"
                     /locus_tag="KPH29_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HBG1467161.1"
                     /translation="MELDKNFKFRLQKVLDLKIKDEEEIKMEFAKIQQKKIDIESNLE
                     NLESNYSKYSISKNNDSVQNQKITINYLLALNNSIMDLSEELDKSTNELEKARKQLIS
                     KQIERKSLEKLKEKKYGQYYKEEQLKEQSTNDEFASMSYLRNRQVL"
     gene            6331..7536
                     /locus_tag="KPH29_001543"
     CDS             6331..7536
                     /locus_tag="KPH29_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HBG1467162.1"
                     /translation="MKIQADSYNIIKDIGNRKSSSKNKNIKNNKFEDNLYKATDHKKV
                     KDRRETSKQNVSSDKRENSDNECHLDEDKSNHLEDENTYEKDCSNNNEALHQLLNLLK
                     DNYKKDKDINTILNNLKKLSISDELKQELKLNKNILNKIANQNLELNRASTNNPNDIE
                     NLDIMKLRELLENNASEDIEVISKTTENDTKSIDLYSFDSNRIDTLNSNKQKDDSSDI
                     LEKLAGVNGNKSGNFGQVINKSVDMNKIKNDNVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENMSKQNTNDKRNNVVIEDEIKENII
                     DDDRVDLLA"
     gene            7554..8162
                     /locus_tag="KPH29_001544"
     CDS             7554..8162
                     /locus_tag="KPH29_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgD"
                     /protein_id="HBG1467163.1"
                     /translation="MYGIATALAGTQVNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMND
                     KLDILLALSNSTLINSALSMATSLIGKEVEVSGEEEEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVGVKYEKLLKIGEVDANSGEENDSSEQESPEEV"
     gene            8189..9172
                     /locus_tag="KPH29_001545"
     CDS             8189..9172
                     /locus_tag="KPH29_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HBG1467164.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMTQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGVIYDGEYKADT
                     GEFIKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLKGTIQKVQTYAFQNPAGLDAEGGNLFKPSANSGDPQIAGAT
                     GNIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            9185..9388
                     /locus_tag="KPH29_001546"
     CDS             9185..9388
                     /locus_tag="KPH29_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HBG1467165.1"
                     /translation="MIRVTDLNGRRYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKVNVGYIRSEK"
     gene            9388..10206
                     /locus_tag="KPH29_001547"
     CDS             9388..10206
                     /locus_tag="KPH29_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HBG1467166.1"
                     /translation="MKKTDSLTFIGLILSTVLVLFGAVKGSSSGLKSFFDFASILITV
                     FGSFGALMITFTIDDIKLIKDALQYSFKTMNISKLDLLNQFKTLSKKARREGLLSIES
                     DVMKIQDPFMKKGLELCIDGLEVNEIRSILELEIDSVEETNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLSDLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            10196..10891
                     /locus_tag="KPH29_001548"
     CDS             10196..10891
                     /locus_tag="KPH29_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBG1467167.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGISMFSTGSSTSEKSPIESVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKADSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPRLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            10903..11346
                     /locus_tag="KPH29_001549"
     CDS             10903..11346
                     /locus_tag="KPH29_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HBG1467168.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQIDKIAGLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLKNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            11361..11735
                     /locus_tag="KPH29_001550"
     CDS             11361..11735
                     /locus_tag="KPH29_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliO/MopB family protein"
                     /protein_id="HBG1467169.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYIKILDI
                     VNISKNNSIIILKIGEEGCIMASSSSSMNKIKDLTKEEILRIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            11736..12401
                     /gene="fliP"
                     /locus_tag="KPH29_001551"
     CDS             11736..12401
                     /gene="fliP"
                     /locus_tag="KPH29_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBG1467170.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLKQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            12414..12683
                     /gene="fliQ"
                     /locus_tag="KPH29_001552"
     CDS             12414..12683
                     /gene="fliQ"
                     /locus_tag="KPH29_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBG1467171.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMMSLITK"
     gene            12695..14509
                     /locus_tag="KPH29_001553"
     CDS             12695..14509
                     /locus_tag="KPH29_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HBG1467172.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYGINETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLIDNLMNWTARMIFFSMDGHHVLIRGIRYSFELIPIGKSFVDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIIGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSTDKTEEATPKKKGEQRKKGNIAKSK
                     ELPVAMTLLAFTLLVPTLFSYVVDTLKSSLNYFLSLDFYMNINYSSIEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIVANLFQVGILFTGETLKPNLSKLNPVSGFKNMFSMRSLSTLI
                     KDTAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIVIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEIKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNDIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVLKR"
     gene            14518..16593
                     /gene="flhA"
                     /locus_tag="KPH29_001554"
     CDS             14518..16593
                     /gene="flhA"
                     /locus_tag="KPH29_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HBG1467173.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMMIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTIGDGLVSQIPALLISTSAGIIVTRASTDENFGGLVSKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAEIKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDDDKTIVVATLSPETEELVSNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMNNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            16568..17584
                     /gene="flhF"
                     /locus_tag="KPH29_001555"
     CDS             16568..17584
                     /gene="flhF"
                     /locus_tag="KPH29_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HBG1467174.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIQGIFS
                     KKDIELTIGWEKRENVEQKDLKREIEQLKLIINNMGFDNKNDNDIDKICKNLLSLELN
                     EEIVESIRIDLQEMKFNGIDTSKNLVEILKKKIKIENQAINGKIALVGPPGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDEM
                     KDCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVVNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            17581..18459
                     /locus_tag="KPH29_001556"
     CDS             17581..18459
                     /locus_tag="KPH29_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HBG1467175.1"
                     /translation="MIDQAQILRKMAMEKRGLDEFIVENENVPKIITIASGKGGVGKS
                     NLATNLSICLTKLNKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNIISGGSALNHIEDFTEAQRNKFIHSIEQIHDVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKRSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVSLCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            18473..19174
                     /locus_tag="KPH29_001557"
     CDS             18473..19174
                     /locus_tag="KPH29_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HBG1467176.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDAEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELNVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            19190..19612
                     /locus_tag="KPH29_001558"
     CDS             19190..19612
                     /locus_tag="KPH29_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBG1467177.1"
                     /translation="MIQIILIVVSILIIVTIVINLHKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEIRSNNHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELSLIRSCYKI"
     gene            19597..19782
                     /locus_tag="KPH29_001559"
     CDS             19597..19782
                     /locus_tag="KPH29_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBG1467178.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            19823..20596
                     /gene="flgG"
                     /locus_tag="KPH29_001560"
     CDS             19823..20596
                     /gene="flgG"
                     /locus_tag="KPH29_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBG1467179.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNDYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            20617..21378
                     /gene="flgG"
                     /locus_tag="KPH29_001561"
     CDS             20617..21378
                     /gene="flgG"
                     /locus_tag="KPH29_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBG1467180.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGNDISTGTGAKATAPMRIYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNIDGLGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            21401..22282
                     /locus_tag="KPH29_001562"
     CDS             21401..22282
                     /locus_tag="KPH29_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBG1467181.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMIVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVIKDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNEYIGSVSLCFPYNGLEALLESISVIEFFDNDVGDDSEEFTKKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            22285..22659
                     /locus_tag="KPH29_001563"
     CDS             22285..22659
                     /locus_tag="KPH29_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBG1467182.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELNTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            22691..24094
                     /locus_tag="KPH29_001564"
     CDS             22691..24094
                     /locus_tag="KPH29_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG1467183.1"
                     /translation="MVLILNLNDIKKDILKLNKKDSCSLYNESINNINNNCISTGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDDAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFEKYNQLIRLINKGMYKEAIQGFKLITDQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNTLDMYNSANIQTKSNVKVDDYKNEFLHKSSSNHKIND
                     YNNVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIAIILGILLVGQSIYSSYKMNNLTDK
                     ISKNNETKNEESIDSNLTKKENNIEENEDMSKASNINKSNKNINKEADVKSKKNTVEN
                     EDDKDSDLFTEDELMSKANTLKSEKKTKSSIQLYKKVADIGENKGNTSEATYQVAILS
                     EKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYYNNGDLKNSKLTLKKLVNKV
                     PNSMYNNSKVKEILKEE"
     gene            24210..26147
                     /gene="htpG"
                     /locus_tag="KPH29_001565"
     CDS             24210..26147
                     /gene="htpG"
                     /locus_tag="KPH29_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBG1467184.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDARNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITIQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
     gene            26535..27665
                     /locus_tag="KPH29_001566"
     CDS             26535..27665
                     /locus_tag="KPH29_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG1467185.1"
                     /translation="MNFNYNLPVNILFGRGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDIDIERFGNISKVLGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
     gene            27892..28914
                     /gene="splB"
                     /locus_tag="KPH29_001567"
     CDS             27892..28914
                     /gene="splB"
                     /locus_tag="KPH29_001567"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HBG1467186.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
     gene            29096..30007
                     /locus_tag="KPH29_001568"
     CDS             29096..30007
                     /locus_tag="KPH29_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG1467187.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYNTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            30022..30438
                     /locus_tag="KPH29_001569"
     CDS             30022..30438
                     /locus_tag="KPH29_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBG1467188.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(30532..30924)
                     /locus_tag="KPH29_001570"
     CDS             complement(30532..30924)
                     /locus_tag="KPH29_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1467189.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            31065..31460
                     /locus_tag="KPH29_001571"
     CDS             31065..31460
                     /locus_tag="KPH29_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBG1467190.1"
                     /translation="MKALEFLNKAGVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
     gene            31863..32069
                     /locus_tag="KPH29_001572"
     CDS             31863..32069
                     /locus_tag="KPH29_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467191.1"
                     /translation="MSKYSSLWEYVKKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFNKID"
BASE COUNT        13255 a         3414 c         5922 g         9714 t
ORIGIN      
        1 atagcaactt cattttttat taataagcaa aataaataaa atatattttg aggaggagtg
       61 agtgtgaata catatgacat tataaaaatg ggattagatg caactaattt aagaagtcag
      121 acaatagcaa ataatatagc caatataaat actgaaggat ataagagaca ttatgtaact
      181 tttgaagaaa cattaaatga tgctatgcca aaaatagaag aaaaaacaga caattctaaa
      241 ggattaagag tggatggcaa taatgtagat atagaagaag aaaaagtaaa tcaagcaatg
      301 gcaacattac aatataatgc tttgataagt tttgcaaatg gaaaaatagc aatgactaaa
      361 tcaataataa gtgggaggta attaaaaggt gagtgtgttc agtggaatga gaatatctgc
      421 tagtggatta acagcagaaa gcttgaaaat ggatatcata tcgtccaatg tagctaatat
      481 aaatgccaca agaggtccaa atggagaagc atataggaga aaagttgttt catttgaaga
      541 aaactatgat aataaattag gtatgctagg agtaaaagtt acatctgttg aagatgataa
      601 atcacctctt aaagaagtat atgacccatc tcatccagat gcaaagccaa atggatatgt
      661 agaatatcca aatgtaaata tattggtaga aatgacagat ttaataacag cttcacgagc
      721 atatgattct aacgtagata cattgaatgc tcagaagtct atgatatcaa aagctttgga
      781 aataggaaag tagggataaa ttatgccaat tacacaaatt aacaatttaa tgcaaggtgc
      841 aaagttagat aatttgattg gagaaacaaa accattaact cttaataata aagaagaaaa
      901 aaagaacttt gaagatgtaa taatagatac tatagataaa gttaattttc aacaagtgac
      961 agcagatgat atgaaagaaa aatttataaa aggcgaagat gtttctatgc atgaagttat
     1021 gttaataggt caagaagcac agctatctat gcaatattta atagaagtta gaaataagat
     1081 tcatgatgca tatcaagaaa tgaataaggt acaaatttag ccttttgaac aggagtaata
     1141 atcaatgata gccagtttta aaaataagat taaagagcaa gttgctaaat ctaggacaaa
     1201 gataagttct ctaaaaaagg gaactaaaat agcattaggt ataggggtta ttgctatatg
     1261 tttagcgcta gtatttacag caatatctgc gagcaaaaat aagtatgcag tcttattttc
     1321 agaactagat gttcaagatg ctgcagatat atcagcagaa ttagagtcta aaaagataga
     1381 tactaaggta aagggaagta cgatttatgt acctagaaca gaagttgata agttaaggct
     1441 ggagctagca ccaagtctta caggtggttc taaaggattt gcactaatgg atgatagttc
     1501 atcaatagga cttactgatg aagagtttca gattaagaag caaagaatga tacaaggtga
     1561 aatagaaaaa acaataaaga gttttccaca agtggaagat actagagtac atataacatt
     1621 tggagagtca tcagtatttg agaaggaggc tattccaggg agtgcagcag tttatttgat
     1681 gttaaaacca gggacgactt tggaaaaaaa ccaaataaaa tctgttatgg cacttatatc
     1741 aggaagtgtt acaaatatac cagagaaaaa tgttcaagtc attgataaca aaatgaattt
     1801 attatctgaa ggtattttag aggatgaaga tgttgatgaa aataatcaac caacaaatag
     1861 tttgattaat gttgataaaa ataaggcatc tgaacaagca ttgaataaac aattagaaaa
     1921 gtctatttta gaaatgctag aaccaatatt tggtaaagga aaagtaaaag ccacagtaaa
     1981 tgctgattta agttttgata cagctgaaaa aactgagata aaaattgacc ctgataaagt
     2041 tgcaataaag gagacaaggt cgaaaaattc atctaaatct gctacaaata atgttcaagg
     2101 tgtagatgct aacatgaata accaaggaaa taacaatggt actaatacag aagatagttt
     2161 agatgagagt tttgagtatg aaacaggtaa gattgaaact cacactatag ttgcgcctgg
     2221 agaacttaat agattgacag cttctgttgc aatagatgga aaaatagcta aaggtgtaca
     2281 agctgatgta gaggatattg ttaataatgc tataggtatg gatggagcga ggggagatac
     2341 tcttagcgtt gtttcaatgg tatttgaccc agaagggaag gcagaagcta aacaagagct
     2401 agaagagatt aaaaaagaag gatttaaaaa gaacataata aaagcagtta ttggagtagt
     2461 ggcagtggta gcaattggag ctattggata cttaatatat aagaaaagaa gtgaacgaga
     2521 agatatggat tatgaagatg aagattatat ttctaaatct gattctgttg ataggaagga
     2581 taatcctcta ttaggagatg acccagaact gacattagag gaagctgtta aaatgtatgc
     2641 agaagaaaag ccagaccaag taacagagat aataaaaact tggttaaatg agtaaggggg
     2701 atagaaagtg tccacggaaa ttacaaatgt taatgcgttt gacttaagtg atataaatga
     2761 aagtgataag aaagagaaga tagaaggtga gggtttaaaa aaagctgctg tattattaat
     2821 gacattagga ccaaatgtgt ctagtgatat aataaaaagc ttgcctgata aacaagttca
     2881 aaaaataggt ttagaaattg caaatatttc ttcaatagct tcaaaagaaa gaagagaaat
     2941 cttaaaagag tttgtagaga taaatagggc aaaagattat gttatagaag gtgggcttga
     3001 atatgcaaga gagttgttaa gcaatgcttt aggaaatgct aaagcaaaca aattacttga
     3061 aggtatatca ataaatacaa gtacaaagcc ttttaaacat attataaaac tagatgtaaa
     3121 gaaaatatta gaaatactta gggatgaaag tgcacaaact atagctgttt tacttgcaca
     3181 tatggagcca gaaaatggag cagaagtttt atcatactta gattttgatt tacaaaatga
     3241 agtagctatg agaataagct caatgggttc aatatctcca gaagtaataa aggtaattga
     3301 aagttcatta gaagataaat tacactcaat aagtggtgat gattttaagg attatggagg
     3361 agtgtctacc ttagttcaaa tattgattaa ttctgataga aaaacagaaa agaatatttt
     3421 gggaactatt gaagaacaag acccggcttt agcagctcaa attaaatctc atatatttgt
     3481 ctttgaagat attgtcaagt tagatgatat aagtatacaa aaagtattaa aagaagtgac
     3541 tatgaaagat ttagtatatg ccttaaaggg tgtggatgat gagatttcag atgctatata
     3601 tagaaatcaa tcttctagag cagcagatgc acttaaagaa gaaatggaaa tggttggtgt
     3661 cataaaagtt acacaggttg aggctgctca acaaaagata gcaaatatag taagaaaact
     3721 tgaaagagaa ggtgccataa ctattgtaag gaattttggt ggtgactttg ttgaataata
     3781 agattattaa aagtaaaaaa gcatcatata aaggagtgct agatttaacc ataatagata
     3841 aagttagagt gggagaatca aatgtaaata aagaaaatga ttgtagattt agagatgagg
     3901 taataaaaga agctatagaa tctgctgaga taaaaaagaa tgagatatta gaagaagcaa
     3961 aaatgaatgc acaaaatata gaaaaacaag catatgaaaa agggtatagt caaggtcaaa
     4021 aaaatggata tgaagatgga tataaagagt catatgatga atatacccaa aaagctaaag
     4081 atgaagcaga ttttataaaa aatcaggcag acttaatact attacaagct aatgagaaga
     4141 tgactgagta cataaaagaa aaaaataaag agattgtaag acttgctatt aatatggcta
     4201 gttctgttct aaaaaagcat ttcgaagatg aagaatctat gagtgatttg ttaaagcaaa
     4261 ttattcaaga ttatgaagga agtagttcat ttatagtaag atgtaattct ttctataaag
     4321 ataatataga gaaagagttt gaattattca aagaggaaac atcactaaaa tcaagtgtat
     4381 ttgttattgc agatgatagt atagataaag gcaatgctat catccaaaag gaaaatggaa
     4441 gaataatagt tggtatcgat tgtgctatgg aaaaactaaa agaagaattg cttggtgaat
     4501 aaagtatgta taaaactgat atatctatag attttgacaa aataaataag aaaatagaaa
     4561 atttagaact cactatatca gaaggtagag taaagaagat aataggactt actgtagaag
     4621 ttgaaggaat aaaagctttt gttggggagt tatgtgttat atacaatcaa gtcaataaac
     4681 cagtgaattg tgaagtagtg ggttttaaag ataaagaagt catattaatg gcgttgggag
     4741 agctaacatt gatagctcct ggttgtaaag ttatttctaa gggaatacct ttaagtgtta
     4801 tgtgtagcga taatttacta ggtaaagtac ttgatggtct tggaaatcct atagacaact
     4861 cagatgctgt tttaggagat agatacaact tgaacaatga acctcctgac ccaatgaaga
     4921 gaaaaaagat acgaaatata atggaaacag gtgtaagagc aatagatgca tttactactt
     4981 gtggtgaagg acagagaata gggatatttg caggaagtgg agtaggtaag agtacgactc
     5041 taggtatgat tgctaggaat gcaaaagcag atgtaaatgt aattgccctt attggagaaa
     5101 gaggaagaga ggtactagat tttattgata aagatttagg tgaagaggga atgaaaaaat
     5161 ctgttgttgt atgtgcaaca tcagataaag ctcctttggt taggctaaaa ggtgctctta
     5221 ctgctactgc aatagcagag tattttagag accagggaaa aaaagtaata cttatgatgg
     5281 attcagtaac tagatttgct atggcacaaa gagaagttgg tatagcaata ggagaaccac
     5341 cagctcaaaa aggatataca ccatctgtat ttgctatttt accaaaactt atggagagaa
     5401 cagggacatc agataaaggt tctataactg cattttatac agtattagtt gatggagatg
     5461 actttaatga gccaatagca gatacaacaa gagggatact tgatggtcat atagttttat
     5521 ctagagactt ggcaaataag aatcattatc cttctataga tgtattgaac agtttaagca
     5581 gacttatgaa tgagattgct tcaaaagaag acataaaaat agcatctttt gcgagagata
     5641 tgcttgctga gtatagagaa gcagaagatt tgataaatat aggtgcatat gcttcaggta
     5701 caaacaaaaa aatagatgaa gcaatctact atcatgagca tattattaat tttttaaaac
     5761 aaggaataaa tgaaaagagt tcttttaatg aaactataag tagtttaaga agaatctttg
     5821 aataaacaat ttaaaaaaat atttgaatag gttgtgatgg aattggataa aaattttaag
     5881 tttagattac agaaagtttt agacttaaaa ataaaagatg aagaagagat aaaaatggaa
     5941 tttgctaaga ttcaacaaaa aaaaatagat atagagagca atttagaaaa cttagaaagt
     6001 aattacagca aatattctat aagtaaaaat aatgatagtg ttcaaaatca aaaaataact
     6061 attaactatt tgttagcttt aaacaactct ataatggatt tatcagaaga gctggataag
     6121 tcgacaaatg aacttgaaaa agctagaaag cagcttataa gcaaacagat agagagaaag
     6181 tcattagaaa aattaaaaga aaaaaaatat ggacagtatt ataaagaaga acagttaaaa
     6241 gaacaaagca ctaatgatga gtttgcaagc atgagttatt tgagaaatag acaagttcta
     6301 taaaatagta ggaagtgagg atattattgt atgaagatac aagcagattc ttataatatt
     6361 attaaagata ttgggaatag aaaaagctca tctaagaata aaaatataaa aaataataaa
     6421 tttgaagata atctatataa ggcaacagac cataaaaagg ttaaagatag aagagaaaca
     6481 agtaagcaaa atgttagttc agataaacgg gaaaatagtg ataatgagtg tcatttagat
     6541 gaagataaaa gtaatcactt agaagatgaa aatacatatg aaaaagattg tagtaataat
     6601 aatgaagctt tacatcaatt actgaatctt ttaaaagata attacaaaaa agataaagat
     6661 ataaacacta tattgaataa tctaaaaaaa ctaagtatat cagatgaatt aaaacaagaa
     6721 cttaagctta ataaaaatat attaaataag attgcaaatc aaaatttaga gttaaataga
     6781 gctagtacaa ataaccctaa tgatatagaa aatttagaca taatgaagtt aagagaactt
     6841 ttagaaaata atgctagtga agatattgaa gtaattagta aaactacaga aaatgatact
     6901 aaaagcatag acttgtacag ttttgacagc aatagaatag atactcttaa ctcaaataaa
     6961 cagaaagatg atagttctga tattttggaa aaactagcag gggtaaatgg aaataagagt
     7021 ggaaattttg gacaagtaat aaataaaagc gttgatatga ataaaattaa aaatgataat
     7081 gttaatttaa aacagataga atcaaatgta atggatgatt ctataaaagc tattaagcat
     7141 atgaaaacaa atgatataca agaattgaca attaaattaa gaccaaaaga attaggagat
     7201 atgaacatac aattgttaaa agatggtgaa agtatgagag ctgttgtgac tgttttcaac
     7261 aaagatgtct ttgactctat aaataaaaac atagcagatt taaagcaaca tcttgaactt
     7321 acaaatgtaa atataaaaga tgtatcagtg caaatgcata gcgataatac aaatacatct
     7381 gatacctttg ataaagcatt tgaacaacag aatagacaaa ataaccaaga aaacatgagt
     7441 aagcaaaata caaatgataa gcgtaacaat gtagtcatag aagatgagat taaagaaaat
     7501 attattgatg atgatagagt agatttacta gcttagtaag ggggaagtat tgcatgtacg
     7561 gaatagcaac ggctttggca ggaactcagg taaactatag gtcaaataat ttaagccaac
     7621 ttgaaagaaa tattaatgat attgccaata aacaagttag aagtaaagga aatcagacag
     7681 taacagacaa tgggactcca attataggac aaggcgatga tgctgataaa gatttgttta
     7741 tgaagattct tgtagcacag atgggtaatc aagacccaat gaatccacaa gacccaacag
     7801 aatatgtatc acaattagca cagtttgcaa ctttagaaaa gatgtcagct atgaatgata
     7861 agctagatat acttttagcc ttaagcaatt ccactctaat aaattctgct ttgagtatgg
     7921 caacatcatt aattggtaaa gaagtagaag tatctgggga agaggaagaa gatgaaaaca
     7981 ttgtaggagt agtaaaatcc acatatataa aagatgggga agtacactta gaattagaag
     8041 tagatggaag tgacaaaact gtaggtgtta aatatgaaaa attacttaaa ataggagagg
     8101 tagatgcaaa ttcaggagaa gaaaatgact cttcagaaca agaatctcca gaagaagtat
     8161 aatattaaat gagaaaagag gtaaatttat gataaaagca atgtattcag gtgtaagtgg
     8221 aatgaaagca aatcaaacaa aactagatgt aataggtaac aatgtagcca acgctggaac
     8281 tacttctttt aagaaaagtt cagctagaat aacagattca ttttatcaaa ctgtactata
     8341 tgcaagtgct ccaacagcgg cattaggggg aacaaactta ggacaagtag gtgtaggtag
     8401 taagatatca agtataaata aagatatgac acaaggaaat gtacaaccaa ctaataggcc
     8461 atctgattta atgatagatg gagatggata tcttccagta gtaagacaag gaacagttat
     8521 gtatacgaga gatggttctt ttaatttaga tacaggattt aaagcagctg atggtgcttt
     8581 gaagaatatt aaaggtggaa gacttgtaaa tggtgatgga tatatgttac aaggtgtgat
     8641 ttatgatggg gaatataaag ctgatactgg agaattcatt aaaaagggtg atgctaaagg
     8701 aaaagagcct attgtaatac cacttcaaag tataagtcct aaggatggaa cagttcaaaa
     8761 tgtactagag tataatatat caaaggatgg aacagtagaa tttttattga gtgatggtca
     8821 aagaactcaa tttgtctatg atatacctga tggtgctaag gtaggagata cacctgctgg
     8881 aatagataaa ttaaaaggta caatacaaaa agtgcaaaca tatgcattcc aaaatccagc
     8941 aggactagat gcagaaggag gaaacttatt caagccatca gcaaactcag gagatccaca
     9001 aatagcagga gctacaggaa acataataca aggggcaata gaagcttcca atgtagatat
     9061 agcagaagaa tttacagaaa tgataatagc aagtaggtct ttccaagcaa acagtaaaat
     9121 aatatcgaca tcagacgaaa tacttcaaga aataataaat cttaagagat agtaggtaca
     9181 tattatgata agagtaacgg atttaaacgg aagaagatac atattaaatt cagaccttat
     9241 agtgagaatt gactctgtac cagaaagtag gattctcctt acaacaggag aaaaaaatct
     9301 tgtaaaagag tcagtagatg agatagtaga taaagtaata aagtataaaa gaaaagtaaa
     9361 tgtaggttat attaggagtg aaaaataatg aaaaaaacag attcactaac attcattgga
     9421 ctaatattat ctacagtatt agttttattt ggggcagtaa aaggaagtag ttcaggattg
     9481 aaaagttttt tcgattttgc atctatatta atcacagtat ttgggtcatt tggagctttg
     9541 atgattacat ttactattga tgatataaaa cttataaaag atgcgctaca atactcattc
     9601 aagacaatga atatatcgaa attagactta ttaaatcaat ttaaaacact ttctaaaaaa
     9661 gcaagaagag aaggactttt atctatagaa agtgatgtaa tgaaaataca agacccattt
     9721 atgaaaaaag ggttagagtt atgtattgat ggccttgaag taaatgaaat aaggtctata
     9781 ctagaactcg aaatagattc tgttgaagaa accaataaca gatattctaa actatttaaa
     9841 acttggggga ctttcgcacc tgcatttggt atgttgggaa ctctaatagg tttaatacaa
     9901 atgttatctg accttacatc tccagattta atagcatcgg gtatgggaaa agcattaatt
     9961 acgacatttt atggttcttt acttgctaat atcgctttga acccaattgc atataatata
    10021 gatgagaaga ctgaaaaaga gatttatgta aaggaaatga tgctggaagg tataataagc
    10081 atacaatctg gggagagcag catagtagtt gaggaacgtt tagctacata cctttcaaac
    10141 aatgagaagc tagaaattat gaaatccaat aaaaatacag aaagagcaat gagtaatggc
    10201 gcgtaaaaaa aataagaaaa aggatgagat aaatccagat gcatggttgg ctacttatgc
    10261 agatactata acactgattc ttacattttt tgtgttattg tatgctacat cagttgtaga
    10321 tatggaaaaa tttaaaaaca tagcagatgc gttaaaaggt cagttttctg ggataagtat
    10381 gttcagtaca ggttcaagta caagtgagaa gtctccaata gagagtgttt tagtggataa
    10441 aaatgataag tcaaatttag aacaggattt aaaagataaa gttaataatt ctaacttaca
    10501 aaatagtgta acagtcaaag atgatagtag agggatttta ctagaactag atgattcaat
    10561 tttatttgac tctgggattt ctgaattaaa agcagatagt aaagccactc ttaataaagt
    10621 atatgacatg ataaagatga tgaaaaataa gatagttata gaaggccata ctgataatgt
    10681 acctgtaaaa tcaagtacac atgaatccaa ttgggattta tcttctagta gagcagtaag
    10741 tgttgtaaga tactttgtag aagaaaaggg catggaccct agactatttt cggcaacagg
    10801 atgtggagag tatcaacctt tggtttcaaa tgaaacagca aagggaaggg ctgagaatag
    10861 aagagttaat atacttataa ctattgacta ggaggaagag gtttgttcaa aaataaaaaa
    10921 attattataa ttattttgat actgattatt ttctcagcag gagcaggttt ttttatatta
    10981 ggagaaggtg gaaataaaca aattgacaag attgcaggtt tgtttattcc aacagggaaa
    11041 ggcaaagatg aagtaaagac cgaaaatctt gacttagaag agatggtatt aaagctagct
    11101 gatgaaggtg taagatattt aaaagtatca ataactgtat cttatgattc ttcatataaa
    11161 gatatagaaa aaaatacgcc tttaattagg gataatatta taaattgttt tatggcaaaa
    11221 agggcagatg attttactgc tgataattta aagaacataa aagaagaaat taaaaataca
    11281 ttaaatatac aactaggtga aaatgtaatt caaaatatat atttcacaaa tatagtagtg
    11341 caataaaagg atggtaagac atgatacttt atttactaaa aatattaata tgtatcccaa
    11401 taattttaat attgatagtc ttatctttaa aaattagcca aggaacttta ttaaaaaaat
    11461 ataatgataa atatataaaa atactagata ttgtaaatat aagtaaaaac aatagcataa
    11521 taattttaaa aataggtgaa gaaggatgca ttatggcatc ttcttcctct agcatgaata
    11581 aaataaaaga tttgaccaaa gaagaaatat taagaataga aaacgacatt aaagaaagaa
    11641 attttaaaat tcaagatttt aaaatggaaa gaattaaaaa gaaatatgat ttcagtagat
    11701 atattggaaa atttacaaaa aaggaagata agtaaatgga actattatca aaattacctt
    11761 tggctgatgt accatataca agttcaatgc aattgttttt atttctaact gcattaatgt
    11821 ttttaccatt tgtaatgttt atgatgacaa gctttgtcag aatagttata agtttatcat
    11881 ttttgaaatc agcattagga gcacagcaag caattccaag ccaaattttg gtagggcttg
    11941 caatagtatt gacaatattt ataatgagac cagtattaaa tgagataaac gaaaaggcat
    12001 tacaaccata tatgaaagaa gaagttacta tggaagaagc tatgaaggaa gcagagggac
    12061 ctattaaaga atttttatta aagcaaacca gacagacaga tttagattta tttgtagaac
    12121 aggctggttt gaaagaaaag aaattaactc gtgaaaatat accactatct gttgtagttc
    12181 cagcatttgc tataagtgaa ctaaaaactg cttttcaaat aggtttttta atatatatac
    12241 catttttgat aatagacctt gttgtggcaa gtgttttgat gtctatgggt atgtttatgt
    12301 tgcctccagt catgatatca ttaccgttta agttattatt atttgtaatg gtggatggat
    12361 ggaacttgat agtaaaaaca ttgatattgg gatttggata ggagaaaaat agtatgagtg
    12421 aatttatgat aatgagtgtt cttaaaaatg gcatgtttac aggagctatg attgcagctc
    12481 cagtactcat tgtttctctt gtagttgggc ttttaataag tgtgtttcaa gctacaactc
    12541 aaatccaaga gcaaactttg acttttgtac caaaactaat agcaattctt ttagtagtag
    12601 ctattggtgg aagctggatg ttacatactc ttattagctt tacttcggag atattcaata
    12661 tgatgtctct tataactaaa taagtaggat atatatgatt tttgtattta ttagaatagt
    12721 ggcattcttt ggaacactaa ggatactatt tccagctggt acacctgctt tatttaagtc
    12781 gctctttgca atcattatat caatattgat ttcaagtact atgcaaattg aatatgcaac
    12841 aaatatagac aacattgtat tgtttacctt atatggtata aatgagacca taactggcat
    12901 tatgcttgga tatataacca atctatgttt ttatagtatc agaatggcag gttctatgat
    12961 ggatcaacag atgggattat caatgattaa tatgtttgac ccaaattcaa tgactcagac
    13021 aactttaatt gataatctta tgaactggac agcacgtatg atatttttta gcatggatgg
    13081 tcatcatgtt ttaattagag gcataaggta tagttttgag ttaattccaa tagggaaatc
    13141 attcgtagat aataatatag attatataat aaacatattt gtgcagtgct ttttaactgg
    13201 attcaaaata gcaataccta ttgtactttg tctgcttatg gcagatttta tattagggct
    13261 tatatcaaga agtgtacctc aactaaatgt aatgataatt ggtatgccat taaaaatact
    13321 ggttggtatt gcgttattta tcatatcaat acctttaatt gtcaatcaaa taagtcattt
    13381 attaagtcaa ataccaaaga tgtatgaggg aactttcgca ctagctccta tgttttttat
    13441 gggttcaaca gataaaacag aagaggctac tccaaagaaa aagggagaac aaagaaaaaa
    13501 aggtaatata gcaaagagta aagaattacc cgttgcaatg actctacttg cattcacttt
    13561 attagtacct acattatttt catatgtagt ggatacactt aaatcttctc ttaattattt
    13621 tttgagtcta gatttttata tgaatataaa ttattcaagt atagagaaat tagttatagc
    13681 aggattaatg gattttttta agatattttt accaatagct attccattct tagttttagg
    13741 tatagtcgca aacctatttc aagtgggaat actgtttact ggtgaaactt taaaacccaa
    13801 tttatcaaaa ttaaatccag ttagtggatt taaaaacatg ttttctatga gaagtttgtc
    13861 aaccttaata aaggatacag cgattatatc tattttggct tacataggat atacattctt
    13921 ccaagataat tatttagata tattaaagct aggtaacata tacctgccaa ctttaatgta
    13981 cacagttaaa gatttagtat attcaatatt aagtaagatt tgtgtagcta tgatagttat
    14041 agcagttgca gattatgttt atcaaagata cagtcataaa aagcaactta gaatgacaaa
    14101 acaagaagtt aaagatgaat ataaaaactc agaaggagac ccagaaataa aagctaagat
    14161 aaaacaaaaa caaagacaaa tttcgtctca aagaaccatg caagcagtac caagtgctac
    14221 tgtaatagta accaatccca cccacttatc tatagcagtg agatatgaaa aagggaaaga
    14281 ccaagctcca gtagtggtgg caaaaggtgc agattattta gcttttaaaa taagggaaat
    14341 tgcaaaggga aatgacatac ctataataga aaataaacct attgctaggt tgttatataa
    14401 gcaagtagaa atagaccaag aaattccaga ggacatgtat caagcttttg cagagatatt
    14461 agtagcagta tataaaatca aaaatagata caaagtcctc aaaaggtagg ataagtaatg
    14521 aataagttta atttaaagga aaatatggac gtaatagtgg catttggtgt tgtagggatt
    14581 gttcttatga tgatagtgcc gttacctaca tttatattag atattttact ggcaattaac
    14641 ataagtttat ctgtgctaat tttactaatg acattattta caacaaatgt acttgattta
    14701 tctatatttc caacagtgtt acttgtgaca acactattta gattgggatt aaatctttca
    14761 tcaactagat tgattttaac acagggatat gcaggtgaag ttatagaggc atttggaagt
    14821 tttgttgtag gtggaaatta cgtagtagga ataattatat tcttaattat attaattgtt
    14881 caatttgttg ttataaccaa tggttctgga agagtatctg aagtaacagc tagatttacg
    14941 ttagatgcca tgccaggtaa gcagatgagt atagatgcag atttaaatgc aggagttata
    15001 gatgacaaaa ctgctagaaa aagaagaaaa gagcttcaac aagaagcaga tttttatggc
    15061 tctatggatg gagcaaataa gtttgtaaag ggagatgcca tagcaggtct tatagtaaca
    15121 gcaataaaca taataggtgg aattgtaata ggagctgtaa tgctgaatat gactcttatg
    15181 ggtgcagcac agacatatgt aagacttact ataggggatg gtcttgtaag ccagatacct
    15241 gctctattaa tatctacatc agcgggtata atcgtaacaa gagcttctac tgatgaaaac
    15301 tttggtggat tagtaagtaa acagttaacg gcaataccaa aagcagtaat gatgactggt
    15361 tgtgttctaa tcgtacttgg tatgatgcct ggattaccta aaccagcatt tttttcacta
    15421 ggtataggtg ctatagcagc aggttacttt ttaggaagag aaaagaaaga tactttagaa
    15481 gacttggagt ttgaaaaacc tgatatggta aatccagata aaaatgaagt agaagatgta
    15541 acaaacttaa taaatgtaga atcaatagaa gtagaaattg gatatggttt aatatcttta
    15601 gctgatgaat cagcaggagg agatttactt cagagaatag catcaataag aagacaatgt
    15661 gcaatagaca tgggtatagt cgtgcaaccc ataagaataa gagataattt acagttaaat
    15721 ccaaaccaat acaatataaa aataaaggga aatgtagttg gtgggtatga gataatgcct
    15781 aatatggttt tatgtatgga ccctatgaat caaggattta gtatatttgg aataaagaca
    15841 attgagccta cttttggaat agaggcactt tggatagaaa gtgacaaggt agaagatgca
    15901 gagataaagg gttatacagt tgtagattca tccacaatac ttataactca cttactagat
    15961 ataataaaat caaatgcaca cgaattactg ggaagacaag aagttaagac tattatagat
    16021 gcagcaagag aaaattatag tgctgtagta gatgaattga taccagattt aatgacactt
    16081 ggtgaaattc aaaaagtact taaaaatctg ttaagagaaa aagtaaatat aaaggatagg
    16141 gttacaatac ttgaaacgct tgcagaccag tctagaaata caaaagatat agagcttctt
    16201 actgaatatg taagaatagc cttggctaga agtatttgta ataatttagt tgatgatgat
    16261 aagacaattg tagtggctac attgtctcca gaaactgaag agttggtatc taataattta
    16321 caaaggtctg taaatggaac ttatccagca gttgacccag aaaatacaaa taaaatattt
    16381 gagagtatac aagaagtaat gaacaatgta tatttcaatc ataatatacc agtaatagta
    16441 gtatcaccaa agataagagc tccatttaga aaattagttg aaattgtata tcctaatttg
    16501 actgtattat cattaaatga aatacctaac gatattaaaa tcaaagcaga aggtgtggtg
    16561 agcatatatg atgacgaaaa agtatacagc taacacaatt caggatgcta tgaatctagc
    16621 caaaatggaa ttgggtgaca atattacatt aatagataaa aaagaagtaa gaaagtctgg
    16681 aatacaaggt atcttttcaa agaaagatat tgaactaact attggatggg aaaagagaga
    16741 aaacgtagaa caaaaagatt taaaacgaga gatagaacaa ttaaagttaa ttattaacaa
    16801 tatggggttt gataataaga atgataatga tatagataaa atatgtaaaa atctactaag
    16861 tttagaatta aatgaagaaa ttgtagaatc tataagaatt gatttgcaag aaatgaagtt
    16921 caatggaatt gatacaagca aaaatttagt agaaatttta aagaaaaaga ttaaaataga
    16981 aaatcaagca ataaatggta aaattgctct agtcggacca ccaggagtag gtaaaacgac
    17041 tacaatagca aagttagccg caaaattagt ttttgaagaa aataaaaaag taggagtaat
    17101 aactatagat acatacagga ttggtgctgt agaacagttg aagatatata ctgatataat
    17161 gaatattcct tttaaagggg taataagtcc cgatgaaatg gaattggcac ttgatgagat
    17221 gaaagattgt gatgttgtat taatagatac aacaggtaga ggctataaaa actctatgca
    17281 aatactagaa atcaaaaatc ttatagataa agctgaaact gataatatac atttagttgt
    17341 gaattgtaca acaagagaaa gtgatacaaa agcaattata gattcatata gaaatgtgaa
    17401 ttttaaaagc ttaataatta caaagctaga tgaaacaata acatatggct caatatttaa
    17461 cataatgaat tatgcacaaa aaccaatatc atatattaca acaggacaaa atgttcctga
    17521 tgatattata aaacctaatg aagataagat tataaggtta ctattaggag ttgaaagtat
    17581 atgatagacc aagctcaaat tttgagaaaa atggcaatgg aaaaaagagg acttgatgag
    17641 tttatagtag agaatgagaa tgtacctaag ataattacaa tagcttctgg taaaggcggt
    17701 gttggaaaga gtaatctcgc tacaaatttg tctatatgct tgaccaaact taataaaaaa
    17761 gttcttatat tagatgcaga cataggaatg tctaatatag atataataat gggggtaaat
    17821 gtaaaaggga ctataattga tgtaattaat ggcgaaaaaa atatagaaga tataatttca
    17881 cagactaagt atggtgtaaa tataatatct ggaggttctg cccttaatca tatagaagac
    17941 tttacagaag ctcaaagaaa taagtttata catagcatag aacagattca cgatgtggat
    18001 tttataataa ttgatacagg agctggtatg agcaaaagcc tattgtcatt tatatactgt
    18061 agtactgaat ttttcttaat aacaactcca gaaccgacat cattgactga cgcatatagt
    18121 ttattaaaag ctataagtaa ttttggcata aagagaagtg caaatataat aatcaatagg
    18181 gtaattgatt tagaagaagc aaaatctaca tacaaaagaa taaaaatggt tgtggacaaa
    18241 tttctaaacc ttaatctaga attgtatgga tatataatag atgacaaaaa agtaagttta
    18301 tgtgtaaaaa aacagatacc attcatttta gaatatccta caagtattgc atcaaaatgt
    18361 attatagcca tagcaaagag aatggtaagt cagtcaagag aagatattgt tgataataaa
    18421 agttttctaa gaaagatgtt tagtatattt aaaagctaag gaggcgtagt taatgaatag
    18481 agaagaatta ataaaagaga atatgcctct tgtaaagagt atagcaagaa aattttttat
    18541 accagggaaa ggttttgaat atcaagactt agtaaatagt ggagtcattg gattgattga
    18601 tgcaataaat aaatttgatg ctgaaaaggg ggcaaagttt tctagttact cttatataaa
    18661 aataaaatct gctatattag atgaaattag aaatcaaagt ccaatttcta aacataatct
    18721 aactaaagta aataagtata atagagtggt agaaaagttg caatctaaat tattaagaga
    18781 gccaacttca tatgaaattg caaaagaatt aaatgtttca gaaaaagaac ttcatgatat
    18841 cgaaagtaat atagacatgt taaatattgt atcattaaac tatgtaatct ttgaagatac
    18901 aaatgaaact gtacaagatg taataagtga tagagaagag gaagctccag aaaatataat
    18961 agaagaagaa gaaaagttag agattctatc taaagcaatt agtaatttaa atgaaagaga
    19021 aaaattaata ctttcactgt actattatga agatttaaat ctaaaggaga tagggaaagt
    19081 gctaggtgtt tcagaatcaa gagtttcaca actccacaga aaatcaataa gaaacttaag
    19141 aaacaagata aaagaactta aatattctat atagatggtg attgataaga tgatacaaat
    19201 tatattaata gtagtgtcta ttttaataat agtaactata gttataaatt tacataagga
    19261 atcaaaaaat aaagataaca gatactttga taaaatattt gagcaagaat atgaagcaaa
    19321 tgatagatta ataattgcag aaaaaagaag attcttttta gacaaagtac ttgctgaaat
    19381 aagaagtaat aatcatataa aagtttatga aaattataac ctaataaagg ctaatgaaga
    19441 aagaaaggaa aacatattta tagaatcaaa aaaggaagta agacaagaaa atgagttatc
    19501 tcttaaagtg aaacatctta taagttcagg ttatgatgat gtggaaatat gtaagatgct
    19561 agatattgga aagggggaac tgtcattaat aaggagttgt tacaaaatat aaggatatta
    19621 ttatgtgata aaaaagtatt cttaggaata ggtattggta tattaatatc aactttatgc
    19681 ataatgcttt ttagtaataa tgatatgagt aaggcagaga tagaaaaaaa ggccagagcg
    19741 ttaggcatga aatatacaag tgagatgaaa gttttagaat agatgatact gtttagacat
    19801 ttacaaaaaa ggaggtcata atgtgataag aggattatat acagcagttt ctgctatgat
    19861 aactaatcaa caaaagcaaa atgttgttgt caataaccta gctaatatgg atacaaatgg
    19921 atataaaagt aaacaacttt tgactaaaag ttttgatgaa ttaaagttgg agggttacaa
    19981 taactacgca aatggaacta aacaaaagca gattataggc gatataagtc caggtatttc
    20041 tatggatgaa actatcacaa attttaatca aggacctata aaaactactg ataataaaat
    20101 ggatgtagct attcaaggta agggattttt tctagttagt gatgcagctg gaaatcaatt
    20161 ttacactaga aatgggaact tcagaacaga caatcaaggt gaccttataa caagtgaagg
    20221 gtattatgta cttgggacga atactgctac tggtgctgta ggtcatataa atgtaggaaa
    20281 tcaaaaattt gaagtggcaa aagataatac aatatcacta gatggaaatg ctatgtataa
    20341 attcaatatt gtagatttta atgactacaa taacttaaaa aaagaaggag ataacttgta
    20401 ttctggagga ggagctatga aagtaaataa tgcacttaca atacaaagtg cgatagagtc
    20461 ttctaatgta gatatggtat ctgaaattaa taatatgatg accatatcaa gagaatatga
    20521 ggcaaatcaa aagataattc aggctatgga ttctaaatta gcaaaaatag caagtgagat
    20581 tggttcagta agataggtga taggaggtga atttttatgt acacaatgtt ttatactagt
    20641 aaaacagctt tatcagcaaa tcaaagtaaa ctagatataa tatctaacaa tatagcaaat
    20701 tctgattcaa caggatataa aaaaataaat atggaatatt ctgatttagt ggatgaagta
    20761 ctaaatagac catcatatcc tacaaatggc aatgatatat cgactggaac aggagctaag
    20821 gcaacagctc ccatgagaat ttatgaacaa ggtgcattgg cccctacaga tagtaagagt
    20881 aatttggcga tagatggaga aggattcttt agagtgataa gaaaagatgg tacatatgca
    20941 tatacaagaa atggtggatt taatatagat ggtttaggta agattgtaga tgataatgga
    21001 aatatattag atgttcaatt tgatgcagga tataactata ataatacgaa tatagatagc
    21061 acaaacttaa cagtttcaag aaatggcgag ttattcgtag ataataaaaa aataggaaaa
    21121 atcaatttat atcaacctat aggaactcaa aattttatat ctgaaggaga tagtttattc
    21181 gtcgcaactg atgcagcaca gattaaacaa gttgagaaag tagatatagt tcaaggctat
    21241 agagagaggt caaatgtttc tttacaagaa gaatttgttg acttaatagc cactcaaaga
    21301 gcatttcaaa tgaactcaaa gggaataaag acagcagatg aaatgtggca aatagctaat
    21361 aatttaagag ctaaataatt atgtaaggtg aggaatattc atgaatgata tgaaagagta
    21421 cgattttggc aaacctcaga gttattcaag agaaaattta acttgtttaa attttttatt
    21481 agctgaattt tgtaaaaaat ataaaaacta tataatttat gaacttaagt gtaattctag
    21541 tatgattgtt gatagtatag accaagtaaa ttatcagact tttttagata gagtaacttc
    21601 ttcatgtgta gtagtacaaa atgcaatgga acctgttatg aaaaactttt cttttagaat
    21661 agatagaagt gtagctgata tgtggattga tataacttct ggaggaactg gagttataaa
    21721 ggataatgat agagagctta cagaacttga taaaaaagtg cttttacatc ttatggatga
    21781 tttagtaaga aatatgcact tatttgaagg ttttgaaaat atacaagtat tggatacaca
    21841 tacacatatt aatttaccac aattatgttc tccaactgca ccagtatgcg ttgtagattt
    21901 aaaagtgcta aatgataatg agtatattgg aagtgtttct ctttgttttc cgtataatgg
    21961 tttagaggct ctattagaaa gtatatctgt tatagaattt tttgataatg atgtaggaga
    22021 tgattcagag gaatttacca aaaaaatcta taatagtgta tctaatatag aattatcagt
    22081 tattgcagag ataggaaaag tatcaattaa tgttgaagac ttacttaaac tagaagttgg
    22141 agatgtaata gtaacaaata aaaaaataaa tgattatata gatatatttg tagaaaattc
    22201 aaaatcatat acagcaacac caggatttat atcaagcaaa aaaggtgtaa aaataaatga
    22261 tgctgttgga aaagaggtgt agttatggac aatagtaatc ttatttcaga tgaagaagca
    22321 aagatgttat taaatgataa agttgaaagt gaagttgatg gggatgatga gttagcctta
    22381 gatgtctctg aagttaatcg aaataatata caaaggatac ttgaccttaa gttagaatta
    22441 tctgtagtta tagggagaac taagatgact ctaagagata ttttaaattt acataaaggt
    22501 tctcttattg aactgaatac tttagcagac caagatgtgg aaatactaat agataataag
    22561 gttttggctt atggaaaagt agtggtggta gatttaaatt ttggtgtaaa gataacaagt
    22621 atagtaagtg aagaagacat ggtaaaatct cttgtttaga gataacatca attaatactt
    22681 aaggcatgag gtggtactta ttttaaactt aaatgatata aaaaaagata tactaaaact
    22741 caataaaaaa gactcatgtt ctctatataa tgaatctatt aataatataa ataataattg
    22801 tatatcaact ggattaaaat ttttaaaaca agcaagagat ttaaacccag atgatgttga
    22861 tatattaaat ctcacaggtc ttgtaaactt actaaaatgt aattttgatg atgctgtaga
    22921 aagtttctat aagagttcac tttgtgaaaa aactgctctg tgtgaaaagt atataaacat
    22981 gcttacatct gaggagtttt taattttctt tgaaaagtac aatcaactta taaggttaat
    23041 aaataaagga atgtataaag aagctataca agggtttaaa cttataactg accaatactg
    23101 tgatttaata gaaccttatg agttgcttgc cttactgtat gacaaggaag aagaatatat
    23161 aaaatttgat gaatgtttag aagtattgaa gattattgat aaggaaaatt atttattaaa
    23221 tcaagaaaat aacacattag atatgtataa tagtgctaat atacaaacta aaagtaatgt
    23281 aaaagtagat gattataaga atgagttttt acataaaagt agtagtaacc ataaaattaa
    23341 tgattataat aatgtaacta aagaaaaaag ttcaagcaaa aaccagaaaa gttcagattc
    23401 taataagaaa aaaagtatta aaaaaaataa gtacctatat atagccataa tattaggaat
    23461 attattagta ggacaaagta tatatagttc ttataagatg aataatttaa ctgataaaat
    23521 atctaaaaat aatgaaacta aaaatgaaga gtcaatagat tctaacttga ctaagaaaga
    23581 aaataatata gaagaaaatg aagatatgag taaggcttct aatataaata agagtaataa
    23641 aaacattaat aaagaggctg atgtaaaatc taaaaaaaat actgtagaaa atgaagatga
    23701 taaagattct gacttattta cagaggatga attgatgtct aaggcgaata ctttaaaatc
    23761 agaaaaaaaa actaaatctt ctatacaact atataagaaa gttgcagata taggtgaaaa
    23821 taaagggaat acatctgaag caacttatca agttgctatt ctcagtgaaa agttaaaaga
    23881 ctataaaact gctgaagaat attataaaat gtatgtagaa aattactcag aaaaagatgc
    23941 ttattttgat gaatcttatt ataatttagg gatgatgtac tataacaatg gtgatttaaa
    24001 aaattctaaa ttgacattaa agaagttagt taataaggta ccaaatagta tgtataataa
    24061 ttcaaaagta aaagaaattt taaaagaaga atagttaaat tagataagaa aaataaatta
    24121 ttttatttta aaataatatg attataatat attgacatac cattttaaaa aatttaaaat
    24181 attaattata aaatgaggag ggataaaaaa tggaatttga aaaagggagt atttcaattc
    24241 atacagaaaa tatttttcct ataattaaaa aatggttata ctctgataaa gacatattta
    24301 taagagagct tataagtaat ggatgtgatg cagtaagcaa acacaaaaga cttgtatcac
    24361 taggagaaat atcagaaaat aagtcatcag attataaaat tacagtatct gtaaataaag
    24421 gagaaggtac acttaaattt attgacaatg gaattggtat gactgaagaa gagataaaaa
    24481 aatacataaa tcaagttgct ttttcaggtg ctgaagattt ctttaataaa tataaggata
    24541 agatggaaga gtcaaatgac ataataggtc attttggatt aggattttat tctgcattta
    24601 tggtgtctaa aaaggtacaa atagatacac tttcatatac agaaggagcg actcctgtaa
    24661 gatggatatc tgaaggtggt acagaatacg aaatatctga atcagatgct agaaatgata
    24721 gaggaactac tataacacta tttattgatg atgatagtaa agaattttta gatgaattta
    24781 ctgtaagagg aataataaat aaatactgtt ctttcttacc agtagaaata tatttagaag
    24841 atgttgaaag attagaaaga gaagctaaag aagcagaaga aaaagctaaa aaacaaaagg
    24901 aagatggaaa agaagagatt gtagatgcaa aagtaattga gcctttaaac gacactaatc
    24961 ctctatggtt aaaatctcca aaagactgta ctgatgagga gtataaagag ttttatagaa
    25021 aggtattcaa tgtatttgat gaacctttat tttggataca tttaaatgta gattatcctt
    25081 tcaatttaaa gggtatactt tacttcccta aattaaaaaa tgagtttgaa ctgactgaag
    25141 gaaaagtaaa attatacaac aatcaagtgt ttgtggctga caatataaaa gaagttatac
    25201 cagaattttt acttttatta aaaggtgtta tagactgccc agacttacca ttaaatgtat
    25261 caagaagttt cttacaaaat gacagggatg taagcaaaat ttctaaacat ataattaaaa
    25321 aagtagctga taagttaaaa tctctttgta aaaatgaaag agaagaatac aataagtttt
    25381 gggatgatat acaaatattt attaaatatg gttgcttaaa agatgaaagt ttctatgaga
    25441 aggtaaaaga atgtatatta ttcaaaacta ttgatgatga gtatataaca atacaagatt
    25501 atttagaaaa atgtaaggac aaacatgaaa ataaggtatt ttatgtaagt gataaggaac
    25561 agcaatctca atacattaaa ctatttaaag aatacgactt aagtgctgtt gttttaaact
    25621 catcaataga tactcatttt atttcattta tggaatataa agagaatgga gttaagttta
    25681 atagaataga tgcagacctt tctgatgtat taaaagataa aaatgaaaat aaagatagtg
    25741 aagaaaacaa agaagagata gctaagatag aaggtttgtt taaagaagct gttggagaaa
    25801 gagtgaagaa ttattctgta gaaggattga aaaatgaaga cactccagct atggtattag
    25861 tatcagaaca atctattaga atggcagaaa tgcagtcaag atttgctgga atggatttag
    25921 gaatgaattt tgaggaagaa aaaactcttg taataaatga gaacagtcca ataatcaaaa
    25981 aattggtttc tcttaaagat gatgaggaaa agaaagataa gattacgctt atttgtaacc
    26041 agatagcaga tttagcttta ctttctaata aagaattaaa gccagatgag ttagattcat
    26101 ttgtacaaag aagtaataag cttatgagtc ttttgataga attataagat tgaatttgaa
    26161 tacattgaag caaatttata aaaagtattc aatatcagtg atgagaaaga agtgtctcaa
    26221 aatgaacttt tcagttcatg aggcgctttt tttatgtgaa attaaattta ttttaatcat
    26281 ttcaacaatg aaaaatatat ttatctctat tttgagacag accgttcttt atttttttat
    26341 ctttggttaa tttttaaata tttaagtagg ttctaataaa aaactctatt tagttaaata
    26401 tagttaactt gatatataat aagataatac atataattaa gtaaaagatt gttttttagt
    26461 ttatgtttaa atacataatt acactttgtt aaaaacataa aattattact aaagtagaaa
    26521 taggaggttt tagcatgaat tttaattata atttgccagt aaatatctta tttgggagag
    26581 gcagaattaa tgatgtggga aaagaagtat ctaaatatgg caaaaaagta ttgatagtaa
    26641 caggtaaaaa tagtacaaag aaaactggat tacttgataa aacaatagat ttactaaaag
    26701 actctaaaat agaatatgaa gtttttgata gagtagaaca aaatcctctt ataactacta
    26761 tatattcagg cgtagaaatt ataaaatcaa ctggatgtga ttgtgtttta gggcttggtg
    26821 gtggaagcat tatggatgct gccaagagta tagctttttc ttataaaaac ccaggagatt
    26881 taaatgatta tatatttgga attagacaag gtgaagaagc tttacctata atcttagttc
    26941 caacaacatc aggtacaggt agtgaaggaa attgttttgc agtacttaca aattcagaaa
    27001 ctaaagataa aaaatctata aagaagaata gtatgtatgc aaaagcatca ataattgacc
    27061 cagaacttat ggttacaatg ccaaagcata taattgcatc agttggattt gatgcacttg
    27121 cccataatat ggaagcatat ctttctaatg gaagaaaccc tcttgcagat gtacaagcta
    27181 tttatgggat tgaacttata tcagaaaacc taataaaggt atataatgat gttaatgatt
    27241 tagaagcatg ggaaaaaata acacttgcaa gtactattgg aggaatgacg attggtacag
    27301 cgggtacagc cttgccacat ggaatggagc atcctgtaag tggtcttaga gatgtaatgc
    27361 atggaaaagg acttgcagcg ttgactccta ctattataga aagttcatgg gatattgata
    27421 ttgaaagatt tggtaatatt tcaaaggtat taggaggaac ttgtgcagaa gattgttcag
    27481 atgcaattag aaatttcctt aaaaaaatag atttagatat aaaactaagt gatttaggta
    27541 ttgaaaaatc agatatagat tggttaactg ataattgtat gaaaatatca gttgcaaata
    27601 ttaagagaca tccaaagtat tttaataagg aacaaataaa ggaaatgtac cataagtctt
    27661 tataatttga ctttacatat ttagttttat ataaaaatta taaattaata ataaaaaatt
    27721 ataagtaatt aatagtaaaa aaattgtgta tagaagttat atatataata aacttgtctt
    27781 aagcttttat tttactttct aataaataaa ggagtttaaa aaagggaaga ttaattaagt
    27841 aatattttat aaaattcttc gatttaaaaa ctttaagtag aggtgaaata aatgtttaat
    27901 cccaaaagaa taatattcga aaaaggaact ttagacactg atgtgggcaa aaaaatatac
    27961 aataaattta gagataataa agatgtagag ataattaata tatcatcaaa taggattaaa
    28021 gaacacattc caggtgaaaa tctatatgaa caatatagaa gtgggaagca gactatggta
    28081 gttgggatta gaaaaagtct gaagtttcaa acatgtaaac cttctgctaa ttatcaacta
    28141 ccacttgtca gtggatgtat gggaagatgt gagtattgtt atttgaacac acaattaggt
    28201 gataaaccat ttataagggt gtttgtaaat gtagatgaaa tacttgaaca gacaaaaaaa
    28261 tatattgaag atagaaagcc acaggtaacc atatttgaag gggcagctac atcagaccca
    28321 gttccagtag agccatatac taattctttg aagaaaacaa tagagttttt tggaagagag
    28381 gaaaaaggaa gatttagatt tgttactaaa tacagtgatg tggatagctt attggatgct
    28441 gaacataata atcatacaga aatacgattt agtatcaata ctcctagaat tataaatgaa
    28501 catgagcatt atacagcctc tgcaaataaa agaatagagt cagctgtaaa acttgctagg
    28561 gctggttatc agattggttt cataatagca cctgtgtttt tatatgaagg ttggaaagaa
    28621 gagtataaaa atctgattat aagtataaaa gaagctttac caaaggattt tgataaaaaa
    28681 attatatttg aagttatatc tcatagatat acaactaaag caaaaaatag aatactagag
    28741 atttatccag atacactatt gccaatggaa aatgaggata gaaagtttaa atacggtcaa
    28801 tttggatatg gtaagtacgt gtatgataaa gaacaattag atgatatgaa agagttcttt
    28861 cttaaggaaa taaatggagt atttaaaaat agtttaataa aatatataat atagcaaatc
    28921 ttacctaaag atttgctata ttttattttt atatatttta tgtttataat aaagcaatga
    28981 gatttagcac tatcatataa gaaaattaaa aagggcacta tctatcaaga acattttttc
    29041 tagttttgat actataaagg gtaagagtaa caaaaatatc aggaggagtt atagtatgca
    29101 tgcatgggag tctattcaaa ttacattgga tttaatagaa gcaaatttat cagaagaaat
    29161 atcaattaat gaattagcaa ataaagcaaa tttatcacca ttttactatc aaagactatt
    29221 taaaagattg gtaaataaga ctgtaatgga gtatataaag cttcgtcgtc ttgcaagagc
    29281 ttctgaatac ttagcagaac ataagaatcg tatacttgat gtagctttaa attttggatt
    29341 tgctagtcat gagacattta cacgttcctt taaaaaagca tatggattaa ctccagaaaa
    29401 atatagagca aatccagtaa aactcaatca ctttattaag ccagaattaa ttttaaatta
    29461 tgctatggtt gatgaaggtg ttccactgat tgcagatgat atagtgattg aagtcaatcg
    29521 taagacattg agtaatccaa ggacatttgt tggaatagaa atagaagtac ctatttgcca
    29581 gttggtgggt ggtgaaacaa ctggaattgc aatagtagag gaattatgga tgaaacttgg
    29641 aagtcaaaga cataatatac caaatcaaat tccaggtgga aatgaatttg ctgctcttta
    29701 tatgggaaat gcaaaagaag gaaattgtat gtatatggca ggtgtagagg tagagtcagg
    29761 tacatcagta gaaggatata atacttttga gttgccagct aaagaatatt tagtatgtgg
    29821 atttgaggca gaaaacttta atgagttagt taattcagct gtttttaaag cagataaatt
    29881 tatggaaaga tggatgaaga aacataattt aacaactact gattttgcaa ttgagatgta
    29941 ttaccccact acacctgaag cagcatatct ggaacattgg attgttccgg ttcctattga
    30001 acaatagaaa ggatgatgaa tatgaattgg caagatgcgt tttttcaaga tactaaacct
    30061 acatttgagc aaattaatga atttattaat aatccattat gggacaagtt aaataaaact
    30121 ttggtttcta cttataatgt agaacctaag cttgaataca gtaaatgttc aatgcaaaga
    30181 ggttggaatg taaaatataa aaaaagaggt aaatcactat gtactttata tccacaagaa
    30241 ggttatttta aagctttggt tattgtaagt gaaagcaata gagtagaggt tgatttattt
    30301 attaatactt gttgtgatta tatcaaaaaa atatataatg aggtaaactt ttttaatgga
    30361 agtaaatggc taatgattca agtagatagt ttattggtat taaacgatat gcttgaacta
    30421 attaaattta gagcttaaca tatagaacag gatgaatata taagtcatcc tgtttttata
    30481 ttcaatcaga taaattaata tatagtctat aatatgaata atttagtaaa tttactttat
    30541 ctttctacat tctttgcaac taagtccaca tgtgtttttt tcttgcatat tagtagcacc
    30601 ccaattagca aattgttgta aaataggcac aatactttta cctttacttg ttaatgtata
    30661 ctcaactttt ggaggaattt cattatattg tttacgatga acaataccag tagactctaa
    30721 atcttttaat gattgagcta gcatagtatt tgtaatgcca tccaattttc tttttatgct
    30781 actatatcta attggatttt caactgctaa catacatata ataggtaatt tccacttacc
    30841 accgacaatg tctaatgcat aacggattgg acaggttaat tcttcaacgt ttttagttat
    30901 atttttttca gtatttatac tcattttaat tactccttag ttatagtagt atctttttta
    30961 tactttatca gaaaaaactg catacttgtt ttttctgtat tttatattaa aattatatca
    31021 aaggataatc cttaatacaa gtatttaaaa ggggtgtaag aattatgaaa gcattagaat
    31081 ttttgaacaa ggcaggagtt tattatttag caacagttga tgagaacaat caagcacatg
    31141 tgcgtccttt aggatttgtt atggaataca atggtaaact tacattttgc acatctaatc
    31201 aaaaggatat gtttaagcaa cttattttta atccaaaggt tgaactttgt tgtatagacc
    31261 aaaaccttaa tactctacgt attttaggtg aagcagtatt tgttacatca aaagaaacac
    31321 aagctaaggc acttgaaata atgccaagtt taggtcagat gtactctgtg ggagatggta
    31381 agtttgaaat attctctatt gataaggcaa aagttagttg ttgttcaatg agtggtaaaa
    31441 agattgatgt agagctttaa ttataattga atcgacatcc atagtgaaaa ttaagaaaac
    31501 gttattactg aaatattgat agatttaatt taagtgcaat aaaaagattt ttaaataatg
    31561 aatatgtaaa aatttttagt tattagatgt gtatttcaat aatgatatac tattttgaat
    31621 aattacatac tagaaataag ataattaaaa tttactttat aatgggatag atttctttta
    31681 aaaaatattt taactataat tgatttttat ttaatctgaa aaaaatatta tgactataaa
    31741 aaattgctac ttattatttg gatatttttg tatataaata ataagtagca tgactttata
    31801 cattaataat gatagaatat aaattaatag ataagatata attttaaaag gagtgatata
    31861 atatgagtaa atatagttct ttatgggaat atgttaagaa aaataatagt caatcgttta
    31921 aattaacttt tgaagaaata aaagatattg ctggaataga gatagaccat tcgtttttga
    31981 aatataaaaa agaattaaat gaatatggat atcaagtagg aaaaatctct ttaaaagaga
    32041 agactgttat tttcaataaa attgattaag atataaataa ttacatataa taaaataatt
    32101 tgtattgacg attattacaa ataaaaataa agtaaatctt atataagatt tactttattt
    32161 ttagatgata tttttttagc tttatactat aaggtatata aaactaagcc tattaaaaat
    32221 gttataattt ctattaccat catagaccat atcaatccat caagttcata aaagtaatta
    32281 agctttatta aatagcttct ttatt
//
LOCUS       DAELXX010000035        31251 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.035, whole genome shotgun sequence.
ACCESSION   DAELXX010000035 DAELXX010000000
VERSION     DAELXX010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31251
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.035"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(128..640)
                     /locus_tag="KPH29_001573"
     CDS             complement(128..640)
                     /locus_tag="KPH29_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG1467192.1"
                     /translation="MLEVIKNRRSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKELSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVKQILDLPVKLVPYALISIGYSEEKNEFIDRFDEN
                     KIHRNVYKNR"
     gene            1090..1566
                     /gene="purE"
                     /locus_tag="KPH29_001574"
     CDS             1090..1566
                     /gene="purE"
                     /locus_tag="KPH29_001574"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBG1467193.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDKNLRG"
     gene            1572..2270
                     /locus_tag="KPH29_001575"
     CDS             1572..2270
                     /locus_tag="KPH29_001575"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBG1467194.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            2282..3649
                     /gene="purF"
                     /locus_tag="KPH29_001576"
     CDS             2282..3649
                     /gene="purF"
                     /locus_tag="KPH29_001576"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBG1467195.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            3643..4719
                     /locus_tag="KPH29_001577"
     CDS             3643..4719
                     /locus_tag="KPH29_001577"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBG1467196.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMEQYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAIAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNAAYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            4713..5306
                     /locus_tag="KPH29_001578"
     CDS             4713..5306
                     /locus_tag="KPH29_001578"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBG1467197.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            5299..6831
                     /gene="purH"
                     /locus_tag="KPH29_001579"
     CDS             5299..6831
                     /gene="purH"
                     /locus_tag="KPH29_001579"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBG1467198.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEVLKQKKNLRILKLAKIEDSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKAKP
                     TDAQLKDMEFGMRVVKNMKSNAIAIVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            6842..8092
                     /gene="purD"
                     /locus_tag="KPH29_001580"
     CDS             6842..8092
                     /gene="purD"
                     /locus_tag="KPH29_001580"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBG1467199.1"
                     /translation="MKILVVGGGGREHAICWKLSKEKNVEKVYCAPGNAGITNVAECV
                     NIGDTNIEELLKFAKENEIGLTIVGPEVPLVMGIVDEFEKEGLRVFGPNKKCAQLEGS
                     KAFSKEFMIKHNIPTAKYKEYTNLEEAISEIDSFGYPVVIKADGLAAGKGVVIPENRE
                     DAIATLKEMMSDKKFGAAGDKIVIEEFLKGIETSILAFVDNDTIVPMASAKDHKKVNN
                     YEQGPNTGGMGTFSPSEIYTEELANKVKETVLEKTLEGFKKDGLNFKGILFVGLMITE
                     DGEKVLEYNVRFGDPETQSVLFRLETDLHEIMETILDNKLKDIEINYSDDEAVCVMLT
                     SGGYPDSYEKGKIITGLENLDDDIVVFHSGTKMLDGNLVTNGGRVIGITAKSTTVKDA
                     AEKVYENIKKINFEGMHYRTDIGR"
     gene            8117..11923
                     /locus_tag="KPH29_001581"
     CDS             8117..11923
                     /locus_tag="KPH29_001581"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HBG1467200.1"
                     /translation="MLNTENKDSMVRRVLVEKREGFDLEAKALKKDLVESLHIDNIEN
                     LRILNRYDVEGISEEVYENAAKTIFSEPNLDVVYYEEIPKLNDERVFAIEFLPGQYDQ
                     RGDWAAQCVQIVNQGIRPAINTAKVYILSGKITDEEFSKIKDYCINPVDSREASLEKP
                     ETLKMETEIPTTVEVLDGFIDLDENGLRTFVSEKGLAMTLGDLQHVQKYFKDTEKRNP
                     TITEIKVLDTYWSDHCRHTTFMTEIENVKIEDGKFNDIVKEAYQMYLNSRDNVYVNRH
                     KDICLMDIATVAVKELKKNGKLNDLDESEEINACSINVDVEVDGKMEKYLVMFKNETH
                     NHPTEIEPFGGAATCLGGAIRDPLSGRSYVYQAMRVTGSADPRTTLEDTLPGKLMQRK
                     ITTEAAHGYSSYGNQIGLTTGQVAEVYDENFVAKRMEIGAVIAAVPKENVVRERPEAG
                     DVIVLLGGKTGRDGCGGATGSSKEHSEESILTCSAEVQKGDAPNERKIQRFFRNKEVA
                     QMIKRCNDFGAGGVCVAIGEIADSLDINLDLVPKKYDGLDGTELAISESQERMAVAIK
                     KENKDKFIQLAVEENLEATHVATVTDTGYLRMFWNGKAIVDINREFLDTNGVKQTTDV
                     HVTKVDEENTFFSSNEIVKDVKCASMKDKFTKVLSDLNVCSQKGLVEMFDNTIGGNTV
                     LMPFGGKYQATPTQGMVAKIPVLGGETNTSTIMTYGYNPKVGKWSPFHGALYAVVESV
                     CKLVAIGGNYSTTRLTFQEYFEKLGNNPEKWGKPFSALLGAFYAQSKFEIPAIGGKDS
                     MSGTFKDIEVPPTLVSFAVDTVDAKKVVSPEFKKADSKVVMLCVNKAENDVVDFEELK
                     RNLDKVRELIHGNKVLSTYALGFAGVGEAISKMAFGNKIGFKFSEEAEKTFTDDKLFE
                     ASYGNIVLELANDDLCMLEGYNYVVLGSTTKEASIFIKGEELALDELYKAHCSTLEPI
                     FPTKTEEVKSKIETISYISQGEAKKSSLSIATPRVFIPAFPGTNCEYDSARAFERVGA
                     NANIRVFKNLTYKDIEDSIDTIVNEIKSSQIIMLPGGFSAGDEPDGSGKFIATVFRNP
                     RVQEAINEFLTQKDGLMLGICNGFQVLIKLGLVPYGEIRVPSESAPTLTYNNIGRHQA
                     KIARTRISSNKSPWLAQTNVGDIHNIAISHGEGKFVASEDVMRELIANGQVATQYVDF
                     NNEATYDIEFNPNGSFYAVEGITSADGRVFGKMGHSERIGEEVFKNIIGEKDQKIFES
                     GVKYFR"
     gene            12129..13001
                     /gene="rfbD"
                     /locus_tag="KPH29_001582"
     CDS             12129..13001
                     /gene="rfbD"
                     /locus_tag="KPH29_001582"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBG1467201.1"
                     /translation="MKILITGSNGQLGKELVSQLEAINQSINQPKYVILATTRNDLDI
                     SNQTNIDNFILHNKPDVIVNCAAYTKVDACEDNIEIAYKINALGVRNLAIASEKVNAK
                     LVHISTDYVFNGFSKYPYREDNKTEPNSVYGKSKLMGEKFVEQFSHKYFILRTAWLYG
                     NGNNFVKTMIKLSLENKEVNVVNDQFGTPTSTVDLAKIIIRIMETEHYGVYHATCEGE
                     CSWYDFAKKIFELKNIDIKVNPIKSNEFKCKAQRPLYSVLDNFMLKLIGLNSFRKWEE
                     SIEEYLERYDINEK"
     gene            13110..13991
                     /gene="rfbA"
                     /locus_tag="KPH29_001583"
     CDS             13110..13991
                     /gene="rfbA"
                     /locus_tag="KPH29_001583"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBG1467202.1"
                     /translation="MKGIILAGGSGTRLYPITKCISKQTLPIYDKPMIYYPMSVLMLA
                     GIQDILIISTPRDIKAFEELFGDGSSLGLKITYAIQEKANGIAEAFIIGEEFIGNDRV
                     SLVLGDNIFYGYGFSERLENAVKRKGATIFGYHVSNPNSFGVVEFDKDFNVLSIEEKP
                     TVPKSNYAIPGLYFYDNDVVEIAKLVEPSDRGELEITSINSEYLKRGKLKVELLGRGM
                     AWLDTGTHKGLLDASNYVEVVQTRQGLYIACLEEIAYTKGYISREQLMKLAKPLIKTE
                     YGQYLLKLASEDMQYGR"
     gene            14028..14585
                     /gene="rfbC"
                     /locus_tag="KPH29_001584"
     CDS             14028..14585
                     /gene="rfbC"
                     /locus_tag="KPH29_001584"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBG1467203.1"
                     /translation="MGKINVIKTKIEGLYIVEPNVFCDERGYFMESYNKKDFIEQGLD
                     MCFIQDNESSSRKGVLRGMHFQVRYPQGKLVRVTEGEVFDVAVDLRKDSQTFGKWEGV
                     ILSDKNKKQFYIPEGFAHGFLVLSEKAVFNYKCTNYYYPEFEHGFIWNDKQISIEWPL
                     KGIEEIFLSEKDKKQKSFKDICIRR"
     gene            14623..15606
                     /gene="rfbB"
                     /locus_tag="KPH29_001585"
     CDS             14623..15606
                     /gene="rfbB"
                     /locus_tag="KPH29_001585"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBG1467204.1"
                     /translation="MKKILITGGAGFIGSNFVHYMLNKYVDYFIVNLDLLTYAGNLET
                     LIEIQDSSNYKFVKGDISNREQMYKLFEEERFDIVVNFAAESHVDRSVENPDLFIKTN
                     IIGTQVLLDASIKYGVKRYHQISTDEVYGDLPIDRKDLFFTEQSPINPSSPYSASKAS
                     ADLLVSSYYRTYGLLTTISRCSNNYGPYHFPEKLIPLMILNALENKQLPVYGNGENVR
                     DWLHVYDHCTAIDLIIHKGNIGEIYNIGGHNERSNLEVVKMILNLLDKSEELISYVND
                     RPGHDLRYAIDATKIENELGWKAKYDFDLGIKETVKWYIENESWWKAVLSV"
     gene            15645..16313
                     /locus_tag="KPH29_001586"
     CDS             15645..16313
                     /locus_tag="KPH29_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBG1467205.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCTYNSSSGFDMIMMTLLK
                     ALSQNNQQTSNGYNLSSNSENLFDELGSVVNNTASRFIDVDTKDKSVKSRIENAIEQA
                     SKKYNVDSNLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTDPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMKSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            16322..16750
                     /locus_tag="KPH29_001587"
     CDS             16322..16750
                     /locus_tag="KPH29_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HBG1467206.1"
                     /translation="MGINIDKLNISPSINMEVKKLKESNKFSDKLNSLKQSSSLEDIK
                     RNLESVKKAGERLVLTQNYGDISIYKNAVKEYLKSVVDNMYSLDKNSSFWEHQYYKNV
                     KIVDSKLEDMTSKLISEEKENIDIVSTVDMIQGLLIDMYR"
     gene            16774..17088
                     /locus_tag="KPH29_001588"
     CDS             16774..17088
                     /locus_tag="KPH29_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBG1467207.1"
                     /translation="MNSKKEDIYELNFGQLIEEKSGVIEENQNVFNAKVNVAVSIGNT
                     TETIKNILDLKEGSIIKLSKNVDEKLDIYANDRLFAYGESIITNEKLSVRLSKIEDSD
                     VN"
     gene            17240..17515
                     /gene="flgM"
                     /locus_tag="KPH29_001589"
     CDS             17240..17515
                     /gene="flgM"
                     /locus_tag="KPH29_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HBG1467208.1"
                     /translation="MDIKNVSSNIVVNNYLKNTSGVKKVEEVKKINKTDKIELSKFAK
                     EMASVSNMSDENIRNKKVDEIKNALENGTYKIKPKEIAKKMIEDMRG"
     gene            17520..17936
                     /locus_tag="KPH29_001590"
     CDS             17520..17936
                     /locus_tag="KPH29_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HBG1467209.1"
                     /translation="MITDLKVIIYEEKNIISKMMELLKEQFDYIVEKDLENLNMLNPK
                     LEDISRELASIEIKRRQLFGDDISMSEVVENSNDRYLKEIYTDLKHILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPADKQTATYNSYGNIRK"
     gene            17954..19264
                     /gene="flgK"
                     /locus_tag="KPH29_001591"
     CDS             17954..19264
                     /gene="flgK"
                     /locus_tag="KPH29_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HBG1467210.1"
                     /translation="MAGLFSILNTANSGMNAHQKAIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIARGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGNTVSKYDYY
                     NTIETIINEPSDYGISAGIDDFFKGWESLAKDINSDSKKSLVVENASNLATLISESYK
                     KLNKLKGDIDDNINSEIKQINDMLKSLEDLNKSIDVISGSGSTPNDLLDERDRILDDL
                     SFKLDLENSDVKNMLSDGKLELNELKNADGTWKTGVSGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVTDFFITSNVDGEDIIKVNPAIKSNPNELKLTADEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESVGLDSQKVNQDEVNQRKIINNIDNSR
                     MSVSGVSLDEEMTELIKIQRSYQASAKVMSTAVQLLDVVINGII"
     gene            19283..20215
                     /gene="flgL"
                     /locus_tag="KPH29_001592"
     CDS             19283..20215
                     /gene="flgL"
                     /locus_tag="KPH29_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HBG1467211.1"
                     /translation="MRVSTGMMSSNYLNSLQDNLQRLDKVNRQVNTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYVENCKDTGNWLETTDTALDQLGKLLSDIKKTLVASGDGAY
                     SDDELKTLSNETNEKMKEIANALNATHEGKYIFSGSNTGVPPVECIDNADGSVSLKFN
                     SSLNLNKLNDSLSMDIAQGISVDYNVKLSSLGFDPTKTDPFEELNNISKKLANPDADA
                     IKELTTTCLGDMEQLIENTVNVRSIYGTKANTVEAMKEKNDDSLIQLKDILSQNEEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            20231..20623
                     /locus_tag="KPH29_001593"
     CDS             20231..20623
                     /locus_tag="KPH29_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HBG1467212.1"
                     /translation="MMKVTLKKGILGFENLKEYELLDIEDNDSLKEFNSTEEEGIGFV
                     VVSPFEIINEYEIVLNQETIEKLEVKSPKDVVLFNIITLGQTFEESTVNMKAPIVINI
                     KTNFGMQIILQDDKYSIRHPLLRGDNGC"
     gene            20617..20829
                     /gene="csrA"
                     /locus_tag="KPH29_001594"
     CDS             20617..20829
                     /gene="csrA"
                     /locus_tag="KPH29_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBG1467213.1"
                     /translation="MLVISRKKDEAVLIGESIEVKIVGVDGNNVKLAISAPNNISILR
                     KEIYEKVKSENIKATNKNIKILKSLK"
     gene            20851..21246
                     /gene="fliS"
                     /locus_tag="KPH29_001595"
     CDS             20851..21246
                     /gene="fliS"
                     /locus_tag="KPH29_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBG1467214.1"
                     /translation="MYGENPYNSYKQNAVFMASKEQLLLMLVDGAVKYTKIARAAILD
                     KNTQKAHRELVRVQDIFTELMVTLDQNAGQWAKDMYKVYDFVRYELSRANIRKDIQVI
                     DNVLPVIEQIKDTWHEADKKSKEERSRYK"
     gene            21246..21602
                     /gene="fliS"
                     /locus_tag="KPH29_001596"
     CDS             21246..21602
                     /gene="fliS"
                     /locus_tag="KPH29_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBG1467215.1"
                     /translation="MELNLAELDKLSKEELLLMLVDGTVKYTNISKEALLNNDYLKAH
                     NELIRVQNIFTELMTTLDQDAGQWAKDMYKVYEFIKSELAIADENKDIKIIDDILPIV
                     KQIRDTWHEVYNQLKI"
     gene            21623..23146
                     /gene="fliD"
                     /locus_tag="KPH29_001597"
     CDS             21623..23146
                     /gene="fliD"
                     /locus_tag="KPH29_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBG1467216.1"
                     /translation="MSSISPIRVTGLSGNFDMEGIIEASMIRDKEKVDKAKQEQQIVK
                     WKQEIYRNVIQESKDLYDKYLSVNSPNSIVSEKAYSSTRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKFTIKLNSSEPIVRQFPPNASGASSLTIGDVNIPISEQDTTSTI
                     VSKINSLCADNDIKASYSEMTGELIISRKQTGSSSDINLKVIGNDNLAQQIANDNGIT
                     FANDASGNKVASVYGKNLEADVTDEHGRVTHISKEQNSFNIDNIDYNVNSKGTAKLTS
                     VTDTEEAVKNMQAFVDDYNKLMDKVYGLVTTKKPKDYPPLTDAQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRGFVEDIQSAFFGDGKNIIALRKLGINESENYNKKGQISFNADTF
                     SKALIDDSDKVYKTLAGYSSNYDDKGMFEKLKDIVYEYSGSSTSKLPKKAGIEKTASA
                     SENVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            23149..23484
                     /gene="fliT"
                     /locus_tag="KPH29_001598"
     CDS             23149..23484
                     /gene="fliT"
                     /locus_tag="KPH29_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HBG1467217.1"
                     /translation="MDKLKEKLNLYKDISLQIINLIEKEEYIKISSKLGERQEIINSV
                     SEIDRNDFIQLYNRMELIEIDSRIRDILQGQLLEVKKELHEYKLTKQVNTMYYNLNRE
                     KVNIFNKKV"
     gene            23586..24458
                     /locus_tag="KPH29_001599"
     CDS             23586..24458
                     /locus_tag="KPH29_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HBG1467218.1"
                     /translation="MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETN
                     TAEERQKIADELLQLKDEVERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTTN
                     NNNEIKVALVNTSSIMSKAGITSSTIASLNADGTSGTDAAKQMVSSLDVALKELNTSR
                     AKLGAQQNRLESTQNNLNNTIENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            24551..26692
                     /locus_tag="KPH29_001600"
     CDS             24551..26692
                     /locus_tag="KPH29_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG1467219.1"
                     /translation="MLLSIVMIVKNEEKILEKTLKSLTTLRSSIESELIIVDTGSTDD
                     TINISRKYTENVYFHNWNNDFSSMRNISISYAKGEWLLILDADEILIDSSTIVKFFKD
                     GLDKKFNSASIQLKNLYSYDKKLYGYCSVLRLFKNIDFRYHGKVHEQPIYKNPIFNNV
                     ADFEHYGYLFEDEEIRLNKVKRNEKLLFEELKENDNSPYTNYQLGKNFIILGKYHEAL
                     YYLEKSYELYSKLESAPGYLITSLVKTYLYLGKHKKCEKLCLKYIKKDMNNIDIYYYI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEISTQANSLFSDTDTVGLRDEAIITLIKIYY
                     KLEKYESVISEYNNIEDMEKKKNVYFSLFMSLYKLNRFEDIKNYYKEVSNSKIEKNSF
                     YKNIEEVINNIKDDEKEHVYKILCDIEGNYGILNQIRINKCVSIDKCKEILNEEKEII
                     YAYIIKVAFEDKIDLLDIFYEMEYVWIEKYLKYLLALERSFNLVLYKYIISRPNTSDI
                     KKIRVYKAISKVVLESISLSDEKYKEIFYLYIMYSYKYIRYIYRNLSDFELIKFVCND
                     LDKFTIEFKHLLDIKNKVNDANEKLEYIHSMRKLLNEYPFYNKIIKILISELEENIKE
                     SEEFKDLKDSFIISIENMIETGKINDAKSLVDDYSKTFMDDVKILNIKGILYMFENKL
                     DKADFMLKKALSLDVENEDTIYNIEYLKSLR"
     gene            26716..28593
                     /locus_tag="KPH29_001601"
     CDS             26716..28593
                     /locus_tag="KPH29_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG1467220.1"
                     /translation="MNTPIVVNEKSKDIIKDWQYTYTNEDIKFTSIIILTYNQIEYTK
                     LCIESIRKFTPKNKYEIIIIDNNSSDGTVEWLKGQSDIKSVYNNKNLGFPKGCNQGIE
                     LATGENILLLNNDVIVTPNWLYNLNKALWSQDNIGAVGPITNACSYYQQIDVDYKSIE
                     DMFMFAHKINTSNINLWKYRLKLVGFCMLIKKSVLDNVGVLDELFTPGNFEDDDLSFR
                     MLESGYKLLLCQDTFIHHFGSVSFKENRNKYTDIMYINRRKFETKWGFNPEYSGMIRT
                     DLISKIDDSLEANINVLEIGCGIGATLLEIKNIYKNASIYGIEISESAGKLAKYSADV
                     LIADIEKVKLDYEKDFFDYIILGDVLEHLTDPWKIVNELKQYLKKDGCLISSIPNIMH
                     VSVLRNLVLGNFTYEDSGILDKTHLRFFTLNEITKMFNDSNYIINSVTATSMPLSEID
                     NRFIDTMCKESCKEMRQQYESYQYIVKATKSIDTKRYSSISMVNLKYALMRIDNELDV
                     DSNLSYIFDNYNITEPCFVDDIEYLVDTFVINKATLLNRLGVEAFNRGLGDFSINMFM
                     TVLEMDRDDIDSIYNITSVLCELEEFDTAYNLIKNSSKKVQSESDIQDILYFIEGKRY
                     E"
     gene            28586..30700
                     /locus_tag="KPH29_001602"
     CDS             28586..30700
                     /locus_tag="KPH29_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBG1467221.1"
                     /translation="MNNDKICFITCVNDERLYEECLVYINSLDIPKNFSIETVALRGA
                     DSIASAYNKAMRETDSKYKVYLHQDAFIINKNFIYDILEIFKKNTDVGMIGVAGVKDI
                     PVNGIWWDSDKKAGKVYDSHTGKMDIYEFSNFEECFNEVMAIDGLIMITQYDVFWRED
                     KFDGWHFYDLSQSMEFRQNKYKVIVPKQNKPWCMHDSGVANINNGYDEYRIKFVKEYI
                     NLDLYRENFYEFDKKKASTILKSNKHVMDCMKEELTEQLRLKNYKDVLDIVSKIAHFS
                     VFNHTGLYSCEKCERALLECANNLPEIENTKLFTKKDLKRRKVLHVLSEGYSTGGHTR
                     LVKNWIEIDKDSVHSVVTTWQSDTLPDWLLKAIDDSGGEVISLDLISDDYLKRAVALR
                     KIAYDWADIVILYVHMWDTIPVVAFGVDGKIPILHVNHYDSTFWLGTSIADIVIDFSE
                     ASQLITLDKRGIDKSEILPMPLKEKEVFSKSEVRKRYNLEDDVTIILTIASERKYNSV
                     NDINYFKIAKTLIENTDNTIIIVVGVDTENLYWKTLSNVTDNRVICFGTTANIDDFYK
                     ISDIYLESFMVGSHTSKLDAILYDLIPVKFKNEVSPIFTNMWNFIDKFDYKNIKEVID
                     MIRRYNTSEFDRDKIKEKQSEIKHFVIDKHCIEIKNYIDKIYKEANHHKVKYDLYKND
                     EIEDYQLFVALYGYMVKNKNMS"
     gene            30709..>31251
                     /locus_tag="KPH29_001603"
     CDS             30709..>31251
                     /locus_tag="KPH29_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG1467222.1"
                     /translation="MNNIHINKFEKSCEKNWDYFVENKSINGTFLQTRNFLNYHEEGK
                     FTDHSLIIYKGEEILAVVPACETYENGERVFFSHLGSTFGGIIIRSGFNDIRHVNAIT
                     NKLETYLQEQSFNKVYLKNTSRIFCKEDTSLLEYFLFKNNYMFYDELSMFLELSKYKK
                     DILANFRYSKRRYFKYSLKNN"
BASE COUNT        12794 a         3195 c         5778 g         9484 t
ORIGIN      
        1 aataataaat gatagttaag atttacctta gaggctaatt aggtgttcag tcaaaattaa
       61 tgcctttaga atacaaatgt attctaaagg catttttact atgtattttc atttatatta
      121 ctaaacatta tctattttta tatacatttc tatgtatttt attttcatca aatcggtcta
      181 taaattcatt tttttcttca ctatatccaa tagatataag tgcataagga actaatttta
      241 ctggtaagtc taaaatttgt ttaactttat ttactacttt atttttagga aatactcctg
      301 cccaacaagc agctagacct tgattagtaa cttctaataa taaattttgt gtgcaagcac
      361 ctaaatcttg tatccattta ccaggtttta agaatttatc tctatttcca agcactacta
      421 tacataatgg tgcaaattct atatgctttg ctctatgctg tgactttgag agctctttca
      481 gcagttcctt atcatctact ataataaatt cccaaggttg agcatttttt gatgaaggtg
      541 cttgcatagc tgattttagt atttcagtta ttttatcttc ttctatattt ttatctatat
      601 atgttcttat tgaacgtcta ttttttataa cttctaacat acagattctc ctttaaataa
      661 aattatttct atttatataa tatcattact ttaagtttta gctattactt atataaagaa
      721 taaactttgt gttggtttta tctaagtaaa agtaacattc agaactaaaa aacaagacta
      781 taaatccaag atattaatta aaaaagaagt aataaaaaat aatatacaag atttaaaaaa
      841 gagtattgag cttaaaattt aattagaaaa ccaattttga gataatctgt tttttatttt
      901 aacaaaaaaa cataaataaa gttcgatacg tagcgaatga aaaaaataca agaaatattt
      961 tttttaaaaa atttcaaaaa agagttgata atatatgtat attaatataa aatttaaggt
     1021 gtataattcg tataataata cgaatgattt gtagtgtaaa ataacttata aacggagagg
     1081 ggaagtctaa tgaaagtagc agtagttatg ggttcaaaat cagattatcc aaaattagaa
     1141 gaagggataa aactgcttga aaaatatgga atagaggttg tagcaagagc attatcagct
     1201 catagaacac cagaacaact ttcaatattc ctaaaagaaa tagaagatga tactgatgta
     1261 ataatagcag cagcaggaaa agcagcacac ttaccaggtg taatagcttc acaaacatta
     1321 attcctgtaa taggattacc tattaaatca tcaactatgg atggactaga ctcactttta
     1381 tcaatagttc aaatgccaaa aggaatccca gttgcaacag ttacaataga cttaggttta
     1441 aacgcagctt tattggcatt acaaataatg actttaaaat acccaaaatt aaaagaggat
     1501 ttgaaatcat atagagaaga gatggcacaa aaggtgttag aagacgacaa gaatttaagg
     1561 gggtagcaga aatgttatta tatgaaggaa aagcaaaaca agtatattct acagataatg
     1621 aaaatgaata tgttgtttat tataaagatg atgcaacagc atttaatgga gaaaaaaaag
     1681 cagaaatatc ttcaaaaggt atactaaata ataagatatc aacaataatc tttgaaatgt
     1741 taaaagaaaa taatataaat actcacttta taaaaagttt atcagataga gaaatgttag
     1801 ttaaaaaagt agagatttta ccattagaag taatagtaag aaatattgct gctggttcta
     1861 tctgtaaaag agttggtctt gaagaaggtg tagttttcga tgagccaatt tttgaaataa
     1921 gttacaaaaa tgatgcttat ggtgacccaa tgctaaatga tgattatgca gtggcaatga
     1981 agttagcaac tagagaagag ttaaaattct taagagaaga aactttaaaa ataaacgagt
     2041 tactaaaagc atttttctta aaattaaacc taaagttagt tgactttaaa atagaatttg
     2101 gtaaggattc tgaaggaaat ataatattgg cagatgaagt atcaccagac acttgtagac
     2161 tttgggatgt aaatacaaat gaaaagttag ataaggacag atttagaaaa gaccttggag
     2221 accttgtaga aggttatacg gaagtacttt caagaatgaa taataaatag gaggcaaatg
     2281 tatgtgtgga gttttaggga tttactctaa taaagatgta actaaagaac tatattattc
     2341 tttatattct atgcaacaca gggggcaaga aagctgtggt ttagctcttt tagatgatgg
     2401 agaaattaag tacaaaaaag atatgggatt agttggagat gtttttaagg aaaatgaatt
     2461 atctaagtta aaaggaaata tagggattgg acatgttaga tattcaacag ctggaggaag
     2521 tcatgtttct aactgtcaac ctttagttgg aagttgtaga aaaagacaat tagccatagc
     2581 tcataatgga aatctagtta atgcaaatta tttaaaagat atgctagaag aagatggata
     2641 tatgttccaa actaattcag atacagaagt gattttatat atacttgcta gatattacaa
     2701 aggggatata gtagaaagtt taaaagtgac aatggattat ataaaaggtg catatgctct
     2761 tgtaataatg agtcaagaag aattggttgc agttagagac ccacatggat tcagacctct
     2821 tgtacttggt aaaaagggtg atgaatatat atttgcatca gaaaattgtg caatagatat
     2881 acttggcgga gaagttataa gagatgtaga gccaggagag ataatagtag tcaaagatgg
     2941 agaactaaaa tcatattttt attcagaaaa ttataaacca gttaagaaaa gttgtatatt
     3001 tgaacacata tactttgcaa gaaatgatgc aactatagac aatgtaaatg catatgaatt
     3061 tagaattaaa tgtggtgaga gattagctca gaatgaaact gtaaaggcag atatggtagt
     3121 tccagttcca gattcaggtt ggccaggagc tattggttat gcaaatgcta gtggattaaa
     3181 aataagtgaa ggcttagtta aaaatagata tgtaggaaga acatttataa aacctacaca
     3241 agaagaaaga gaaatagcag taaaaataaa attaaatcct ctttcaacaa taataaaagg
     3301 aaaatctata attttagtag acgactcaat tgtaagagga actacatcta aacaattagt
     3361 aaaatcttta agagaagcag gagcaaaaga aattcatctt agaataactt ctcctccagt
     3421 agcttactct tgttactatg gaatagatac tccaaatcgc tctaaattaa ttgcatcaag
     3481 taacaacgta gaagaaatga gagaatatat tggatgtgat agtttaaaat tcctagatat
     3541 agaaggaatg ttagatgcaa cagaacataa gtctactttc tgtaaagctt gttttgatgg
     3601 agaataccca gttaagaaaa ttgataagga ggaactactt tcatgttaac atataaagaa
     3661 tcaggtgtag atatagatga aggaaataga gcagtagacc ttataaaagg taaaataaaa
     3721 ggaacttatg atggaaatgt agttggagat ttaggtaact tcagtggatt atacagttta
     3781 aaagattttg taggtatgaa agaaccagtt ttgcttgcat ctacagatgg tgttgggact
     3841 aagttaaaaa tagctcagat gatggacaag catgatactg ttggaataga tttagttgca
     3901 atgtgcgtga atgatttaat ttgtcaagga gcaaaaccat tatttttcct tgactatata
     3961 gctcttggga aattagtgcc tgagcatatc gaaaaaatag ttggtggaat tgcagatggt
     4021 tgtaaaatgt ctggttgtgc actaattggt ggagaaactg ctgaaatgcc tggaatgtat
     4081 ggagaagatg actatgattt agcaggattt tctgtaggaa tagcagacaa agaaaagatt
     4141 gtttctggaa acaatgttaa atcaggagat gtattggttg gtatatcttc aagtggagta
     4201 cacagcaatg gattttcttt tataagaaag atatttttag aaacttacaa ttataaaatg
     4261 gaacaatatg ttgaagaact aggaatgaca gttggagaag cacttttaac accaactaaa
     4321 atatatgtta aattagcttt agatgtatta gctaaacatg acataaaagc tatagctcac
     4381 ataactggtg gagggcttat agaaaatata acaagagtta tacctaaagg gttaggacta
     4441 gatatcaata aaaaatcttg ggagaagcct cctatattta aaatgataga aggttttaat
     4501 gctgttgatg agagagaact tcataagagt tttaacatgg gtattgggct agttttaata
     4561 gtagataaag aaaatgctga tgatgttgta aactttataa ataatagaga aaatgacaat
     4621 gctgcttatg tagataaaaa atacagtgaa ttattagaag ataaagcgta tataattggt
     4681 gaagtagtag atagtcatga aggtgtagaa ttatgctaaa tataggagtt ttaatatctg
     4741 gtggaggaac aaatttacaa gcagttatag atggcactga gtctggagaa ataaaaggtc
     4801 aggttaaagt agttatctca agtaagcaag gtgcttatgg gctagagaga gctaaaaatc
     4861 ataacataaa agcaatttgt gaaactgatg aagacaagat aatagagatt cttaaggaaa
     4921 ataaaataga tttagtagtt ttggctggtt atttaaaaat aatcagccca aagctggtta
     4981 atgagtttag aaacaaaatg ataaacatac atccatcatt aattccttca ttttgtggag
     5041 ctgggttcta tggagaaaaa gttcatcaag gtgttataga ttacggagct aaagtaactg
     5101 gtgctacagt tcattttgtt gatgaaggtg ctgatactgg accaatcata atgcaagatg
     5161 tagttaaggt aaatcaagat gatgatgcaa agacacttgc aaaaagagtg ttagaagttg
     5221 aacataggat tttaaaagaa agtataagtc ttttttgtga aaataaatta aaacttcaag
     5281 gtaggagagt gtttataaat gagtaagaga gctttaataa gtgtaactga caagactggt
     5341 gtagtggaat ttgctaagga gttaaataag ttagattatg aaattatatc tacaggaaat
     5401 acatttaaaa cattaaaaga aaatggtgtt aatgtaatgc aagttgaaga tgttacaaat
     5461 tttccagaaa tattagatgg gagagtaaaa acattaaatc catatataca tggtggtata
     5521 ctttataaaa gagataaaga atctcatgta gaaactgtga atgagcataa aatacattca
     5581 atagatttag tagctgtaaa tctatatgat tttgaaggaa ctttaaaagc tggaaaaagt
     5641 catgatgaaa taattgaaaa tatagatatt ggtggaccat ctatgatacg ttctgctgcc
     5701 aaaaactata aagatgtaat agtagtagta gatataaaag attatgattc tataatagaa
     5761 aaattaaaaa cagatacaat gacattagaa gacagaaaaa aattatcata taaagctttc
     5821 tcaactacag gaagatatga tgcgttaata tctagttatt ttgctggaga agttggagat
     5881 acatatcctg acatattaaa tttaactttc caaaaagaac aaactttaag atatggagaa
     5941 aatccacatc aaaatggttt cttatattca caatcaaatg caaaaaatcc aatcttaaat
     6001 tatgagcaat taggcggtaa agaactttct tttaacaatt taaatgattt acatggatgt
     6061 ttagaagtaa tgagagaatt taaagatagt gaagaagtag tttcagtagc tataaaacat
     6121 gctaattcat gtggggtagg tctaggaaaa gatgcttttg aagcatatac aaaatgttat
     6181 gaagcggata aggtttcaat atttggagga atagtaggaa taacttctac tatagataaa
     6241 gctacagcag agaaattaaa tgaaatattc ttagagatag tagtagcata tgattttgag
     6301 ccagaagctt tagaggtact taaacaaaag aaaaatttaa gaatattaaa attagctaag
     6361 atagaagata gtttacaacc atatgaaatg aaatatttag atggaaaatt attaatacaa
     6421 gatagaaata atatattagc agaaaaaagt gaaaatgtaa caaaagcaaa accaacagat
     6481 gcacaactta aggacatgga atttggtatg agagtagtaa aaaatatgaa atcaaatgca
     6541 attgcgatag taaaaaatgg tcaaacttta gctttaggtt gtggacaaac atcaagaatt
     6601 tgggcattaa aaaatgctct tgaaaataac aaagataaag attttacagg agctgtttta
     6661 gcgtcagatg catttttccc atttgatgat tgtgtaacat tagctcatga atatggaata
     6721 tcagcagtag ttcaaccagg tggctctata aaggataaag attcaataga agcttgtgat
     6781 aagtatgata tggttatggt atttacagga ataagacact tcaaacacta aggaggatat
     6841 tatgaagata ttggttgtag gcggtggcgg aagagagcat gctatatgct ggaaactaag
     6901 caaagaaaaa aatgtagaaa aagtttattg cgcgccaggt aatgcaggaa ttacaaatgt
     6961 agcagaatgt gtaaatatag gtgatactaa tatagaagaa cttttaaaat ttgccaaaga
     7021 aaatgaaata ggtctgacaa tagtaggacc agaagtacca ctagttatgg gaatagtaga
     7081 tgagtttgaa aaagaaggat taagagtatt tggacctaat aaaaaatgtg ctcaacttga
     7141 aggaagtaaa gctttttcaa aagaatttat gataaaacat aacataccta ctgcaaaata
     7201 taaagaatat acaaacttag aagaagctat atcagaaata gatagttttg gatatccagt
     7261 agtaataaaa gcggacggat tagctgctgg aaaaggtgtt gtaatacctg aaaatagaga
     7321 agatgctata gcaactttaa aagaaatgat gtcagataag aagtttggag ctgctggaga
     7381 taaaatagta attgaagagt ttttaaaagg aatagaaact tcaattctag catttgtaga
     7441 caatgataca atagttccta tggcaagtgc aaaagaccat aaaaaagtaa acaactatga
     7501 gcaaggtcca aacactgggg gaatgggaac tttttcacct agtgaaatat atacagaaga
     7561 attagcaaat aaagtaaaag agacagtatt agaaaaaact ttagaaggct tcaaaaaaga
     7621 tggacttaat tttaaaggta ttttatttgt tggtcttatg ataactgaag atggagaaaa
     7681 agtacttgaa tataatgtaa gatttggaga ccctgagaca caatctgttt tatttagatt
     7741 agaaacagat ttacatgaga taatggaaac tatactagat aataagttaa aagatataga
     7801 aataaactat agtgatgatg aagctgtatg tgttatgctt acatcaggag ggtatccaga
     7861 cagttatgaa aaagggaaaa ttataactgg tcttgaaaac ttggatgatg atatagtagt
     7921 attccatagt ggcactaaga tgcttgatgg taatttagtt accaatggag gaagagtaat
     7981 tggtataact gcaaagtcaa ctacagttaa ggatgcagca gaaaaagttt atgaaaacat
     8041 aaagaagata aactttgaag gaatgcacta tagaacagac ataggaagat aatttttcta
     8101 aaatggagga taaggtatgt tgaatactga aaataaagac tcaatggtta gaagagtatt
     8161 agttgaaaaa agagaaggtt ttgaccttga ggctaaagct ttaaaaaaag acttagtaga
     8221 aagtttacac atagataata tagaaaattt aagaatatta aatagatatg atgtagaagg
     8281 aatatcagaa gaagtttatg aaaatgcagc aaagacaata ttctctgaac caaatttaga
     8341 tgtagtatat tatgaagaaa taccaaaatt aaatgatgaa agagtatttg caatagaatt
     8401 tcttcctggt caatacgacc aaagaggtga ttgggcagct caatgtgtac aaattgtaaa
     8461 tcaagggata agacctgcta ttaatacagc aaaagtttat atattatcag gcaaaataac
     8521 tgatgaagag ttttctaaaa taaaagatta ctgtataaat ccagtagata gtagagaagc
     8581 atctttagaa aaaccagaaa cattaaaaat ggaaactgaa ataccaacaa cagttgaagt
     8641 attagatgga tttatagatt tagatgaaaa tggattaaga acatttgtaa gtgaaaaagg
     8701 attagcaatg actttaggag acttacaaca cgttcaaaaa tactttaaag atacagaaaa
     8761 gagaaatcca actataacag aaataaaagt attagatact tattggtctg atcattgtag
     8821 acatactact tttatgactg aaatagaaaa tgtaaagata gaagatggta agtttaatga
     8881 tatagtaaaa gaagcgtatc aaatgtatct taactcaaga gacaatgtat atgtgaacag
     8941 acataaagat atatgtttaa tggatatagc cactgttgca gtaaaagaat taaagaagaa
     9001 tgggaaatta aatgacctag atgaaagtga agaaataaat gcatgtagta taaacgtaga
     9061 tgttgaagtt gatggtaaga tggaaaaata tctagtaatg tttaaaaatg aaactcacaa
     9121 ccatccaact gaaatagaac catttggtgg agcagctact tgtttaggtg gagctataag
     9181 agacccatta tcaggaagaa gttatgttta tcaagcaatg cgtgttacag gaagtgctga
     9241 cccaagaacg actttagaag atactttacc tggaaaactt atgcaaagaa aaataactac
     9301 agaagctgct catggatata gttcatatgg taaccaaata ggtcttacaa ctggacaagt
     9361 tgcagaagta tatgatgaaa actttgttgc aaagagaatg gaaataggtg cagttatagc
     9421 agcagtacca aaagagaatg tagtaagaga aagacctgaa gcaggagatg taatagttct
     9481 tcttggagga aaaacaggaa gagatggttg tggaggggca actggttcat ctaaagaaca
     9541 tagtgaagag tcaatcttaa cttgtagtgc agaagttcaa aaaggagatg ctcctaacga
     9601 aagaaaaata caaagattct ttagaaataa agaagttgct caaatgataa aaagatgtaa
     9661 tgattttgga gcaggtggag tttgtgttgc tataggagag attgctgata gtttagatat
     9721 aaacttagac ttagtaccta aaaaatacga tggacttgat ggaactgagc ttgcaatttc
     9781 agagtcacaa gagcgtatgg cagtagctat aaaaaaagaa aataaagata aatttataca
     9841 attagcagta gaagaaaact tagaagctac acatgtagct acagtaacag atacaggata
     9901 tttaagaatg ttctggaatg gaaaagcaat tgtagatata aatagagagt tcttagatac
     9961 aaacggagta aaacaaacta ctgatgtcca tgttactaaa gtagatgaag aaaatacatt
    10021 ctttagctct aatgaaatag taaaagatgt taagtgtgca agtatgaaag acaagtttac
    10081 aaaagtatta tctgacctta atgtttgttc tcaaaaagga ttagtagaga tgtttgataa
    10141 tacaattggt ggtaatacag tacttatgcc atttggagga aagtatcaag cgactccaac
    10201 acaaggtatg gttgctaaga taccagtact tggaggagaa actaatactt ctacaataat
    10261 gacttatgga tataatccaa aggtaggaaa atggagtcca ttccatggtg ccttgtacgc
    10321 agtagtagaa tctgtgtgta aattggttgc tattggagga aattatagta caacaagact
    10381 tacattccaa gagtattttg aaaaacttgg taacaatcca gaaaaatggg gtaaaccatt
    10441 ctcagcactt ttaggagcat tttatgcaca aagtaaattt gagatacctg ctataggtgg
    10501 aaaagacagt atgtctggta catttaaaga tatcgaagtt ccaccaacat tagtttcatt
    10561 tgctgttgat acagtagatg cgaagaaagt agtatctcca gaatttaaaa aagcagactc
    10621 aaaagtagta atgttatgtg taaacaaggc agaaaatgat gttgtagatt ttgaggaatt
    10681 aaagagaaac ttagacaaag taagagaact tatacatgga aataaagttt tatcaacata
    10741 tgcattagga tttgcaggtg taggtgaagc aataagtaaa atggcttttg gtaataaaat
    10801 aggatttaag tttagtgaag aagctgaaaa aacatttaca gatgataaat tatttgaggc
    10861 tagttatgga aatatagtac ttgaattagc aaatgatgat ttatgtatgt tagaaggata
    10921 caattatgtt gtgcttggaa gtacaactaa agaagctagt atatttatca aaggtgaaga
    10981 acttgcatta gatgaattat ataaagcaca ctgtagtact ttagagccta tattcccaac
    11041 taaaacagaa gaagtaaaat caaagataga gactataagt tatatatcac aaggagaagc
    11101 taaaaaatca tcattaagta tagctactcc aagagtattt ataccagcat ttcctggaac
    11161 aaactgtgaa tatgactctg caagagcatt tgaaagagta ggagctaatg caaacataag
    11221 agtattcaag aatttaactt ataaagacat agaagattct atagatacaa tagtaaacga
    11281 aataaaatct tctcaaataa taatgttacc aggtggattt agtgctggtg atgagccaga
    11341 tggttctggt aaatttatag ctactgtatt tagaaatcct agagtgcaag aagctataaa
    11401 tgaattttta actcaaaaag atggactaat gttaggtatc tgtaatggat tccaagtatt
    11461 aattaaatta ggattagttc catatggaga aataagagtt ccaagtgaaa gtgctccaac
    11521 acttacttac aataatattg gtcgtcatca agctaagatt gctagaacta gaatttcatc
    11581 taataaatct ccttggttag ctcaaactaa tgttggagat attcataata tagcaatttc
    11641 tcatggagaa ggtaaatttg ttgctagtga agatgttatg agagaattaa ttgctaatgg
    11701 acaagtagca actcaatatg ttgactttaa taatgaagct acttatgata ttgagtttaa
    11761 tccaaatggt tcattctatg ctgttgaagg tataactagt gctgatggta gagtgtttgg
    11821 taaaatggga cattctgaga gaattggtga ggaagtattt aagaatatta taggtgaaaa
    11881 agaccaaaag atatttgaat ctggagttaa atattttaga taaagctttg atgtttaatt
    11941 gatagctaga atatataatt tagatattta gatttaaaga gatgtaataa tatgatgata
    12001 atcatttaat attacatctc ttttttattt atctaagaaa taataagttc aacctaagtt
    12061 aaaatacaag ctgtgttata attaattact aagtaaaatt aaatatttat tggagggcaa
    12121 cataattaat gaaaatatta ataacagggt ctaatggtca attaggaaaa gaattggtta
    12181 gccaattaga agcaatcaat caaagtataa atcaaccaaa atacgttata ttagctacaa
    12241 caagaaatga tttggatata tccaatcaga ctaatataga taattttatt ttgcataata
    12301 aacctgatgt aattgtaaac tgtgcagcat atacaaaggt tgatgcatgt gaagataata
    12361 tcgaaattgc gtataaaata aatgccttag gagtgagaaa tttagcaata gcatcagaaa
    12421 aagttaatgc taagcttgtt cacatatcaa ctgactatgt attcaatggt ttttctaaat
    12481 atccatatag ggaagacaat aaaactgaac ccaattctgt gtatggaaaa agtaaactta
    12541 tgggagaaaa gtttgttgaa caattctcac ataagtactt tatattgaga acagcatggt
    12601 tatatggtaa tgggaataat tttgtaaaga ctatgataaa actttcttta gaaaataaag
    12661 aggttaatgt ggttaatgac cagtttggaa ccccaacaag tacagtagac ttagcaaaaa
    12721 ttataataag aatcatggaa acagaacatt atggagttta tcatgctaca tgtgaaggtg
    12781 agtgtagttg gtatgatttt gccaaaaaga tatttgaatt aaaaaatata gatataaaag
    12841 taaatccaat taagagtaac gaatttaaat gtaaggctca aagaccacta tattctgtat
    12901 tggataattt tatgttgaag ttaattggtt taaattcttt tagaaagtgg gaagaatcca
    12961 ttgaagaata tttagaaaga tatgatataa atgaaaagta aatgtattat ttttagaaaa
    13021 gaaaataaga tattataaaa aatataaata tttcttttat ttatccgata atcataatat
    13081 gacaaaaaca aatttaagga tggaaaaaga tgaaaggaat tattttagca ggagggtctg
    13141 gtacaagact atatcctata acaaaatgta tatcaaaaca aacacttcca atatacgata
    13201 aaccaatgat atattaccct atgtcggttc ttatgttggc agggatacaa gatatattaa
    13261 taatatctac accaagagat ataaaagcat ttgaagaatt atttggagat ggaagttcac
    13321 tagggcttaa aataacatat gcaattcagg agaaagcaaa tggaattgca gaagcgttta
    13381 taataggaga agaatttata ggaaatgaca gagtgtctct tgtattgggg gataatatat
    13441 tttatggata tggatttagt gaaaggttgg agaatgcagt taaaagaaaa ggagcaacta
    13501 tatttgggta tcatgtaagt aatccaaatt cgtttggagt tgtagaattt gataaggatt
    13561 ttaatgtatt atcaatagaa gaaaaaccta cagttcctaa atctaattat gctataccag
    13621 gattatattt ttatgataat gatgttgtag agatagcaaa gctagttgaa ccatcagata
    13681 gaggtgaatt agaaataacc tctataaaca gtgaatatct aaaacgagga aaactaaaag
    13741 tggaacttct aggaagaggt atggcttggc ttgatactgg tacacataaa ggtctattgg
    13801 atgcttctaa ctatgttgaa gtggttcaaa caagacaggg actgtatata gcatgtttag
    13861 aagagattgc atatacaaaa ggatatataa gtagagaaca attaatgaaa ctagccaagc
    13921 cactgataaa aactgaatat ggtcaatatc tattaaaatt ggctagtgaa gatatgcaat
    13981 atggtagata aaatttatta taaattttat taagggagtt tgttataatg ggaaaaatca
    14041 atgttataaa gactaaaata gaaggattat atatagtcga accaaatgta ttttgtgatg
    14101 aaagaggata ttttatggaa agctataata aaaaggattt tatagagcag ggtcttgata
    14161 tgtgttttat tcaagataat gagtctagct caagaaaagg tgttttaaga ggaatgcatt
    14221 ttcaggttag atatcctcaa ggaaaattag tcagagttac agagggagaa gttttcgatg
    14281 ttgcagttga tttaagaaaa gattcacaga catttgggaa atgggaaggt gtaatattgt
    14341 ctgataagaa taaaaaacaa ttttatattc cagaagggtt tgcacatgga tttttagttt
    14401 tatcagaaaa agcagtattt aattacaaat gtacaaacta ttattatcca gaatttgagc
    14461 atgggtttat atggaatgac aagcaaatat ctattgagtg gcctttaaaa ggaattgaag
    14521 aaatattttt atcagaaaag gacaaaaaac aaaaaagttt taaagatatt tgtataagac
    14581 gttaataaaa atttattaga attaacattt aggagaaaaa atatgaaaaa aatactgata
    14641 actggaggag caggatttat tgggagtaac tttgtacatt atatgcttaa caagtatgta
    14701 gattatttta ttgttaacct agacttgttg acttatgctg gtaatttaga gacattaata
    14761 gaaatacaag acagttccaa ttataaattt gttaaaggag atatttcaaa tagggaacag
    14821 atgtataagc tatttgaaga agaaaggttt gatatagttg ttaactttgc cgctgaatct
    14881 catgtagata ggtcagttga gaatcctgat ttatttatta aaacaaacat tataggaaca
    14941 caagtacttt tagatgcatc tataaaatat ggagttaaaa ggtatcatca aatatctaca
    15001 gacgaagtgt atggtgattt accaattgat agaaaggatt tattctttac agaacaatct
    15061 cctatcaatc caagttctcc atattcagct tctaaagcat cagcagattt gttggtaagt
    15121 tcttattata gaacatatgg attgttaaca actatatcta ggtgttccaa caattatggg
    15181 ccatatcatt ttcctgaaaa attgatacct cttatgattt taaatgcttt ggaaaacaaa
    15241 caattgccag tatatgggaa tggggaaaat gtaagagatt ggttacatgt atatgaccat
    15301 tgtactgcta tagacttaat aatacataaa gggaatatag gggagattta caatatagga
    15361 ggtcataatg aaagaagtaa tttagaagta gtaaaaatga tattaaatct tttagataaa
    15421 tctgaagagt taatatccta tgtaaatgat agacctggtc atgatttaag atatgctatt
    15481 gatgcaacta aaatagagaa tgagttaggt tggaaggcaa aatatgactt tgatttgggt
    15541 ataaaagaaa ctgttaagtg gtatatagaa aatgaatctt ggtggaaagc tgttttatca
    15601 gtgtaacttt gaaatttata aaatttagaa aggggcgttt tatattgaat gagataagtt
    15661 cactaattaa tatattagca ttgactcaat tatctggtag taatactaca aatcaatgta
    15721 actgtacata taatagttca tctggatttg atatgattat gatgaccttg ttaaaagcat
    15781 tatcacaaaa taatcaacag acttctaatg gctacaattt atcgtctaat tctgagaatt
    15841 tatttgacga actcggcagt gtagtaaata atacagctag tagatttata gatgttgata
    15901 caaaagataa aagtgtaaaa tcaagaattg aaaatgctat agagcaagca tcaaaaaaat
    15961 ataatgtgga ttcgaatctt ataaaagcaa taattaaagt tgaatcggat tttaatccaa
    16021 atactgtttc ttctgctggt gctaagggtc ttatgcaact tatgccagaa aattgcagag
    16081 acttgggggt tacagaccct tttaatatag agcagaatat agatgctgga actagacata
    16141 ttaaggaata cattgatatg tttggaggga gcatagaaat gggattgatg gcatataatg
    16201 gtggtcctgg aagaatgaaa agtagaggag tagaatcaat ctctgacctg tataaaatgc
    16261 caaaagaaac acaaaattat attcctaagg ttatgaaata ttatagaggg taggctttat
    16321 tatggggata aatatagata agctaaacat aagtccaagt ataaatatgg aggttaaaaa
    16381 actaaaagag agtaataaat tttctgacaa attaaactct ttaaaacaat cttcttcatt
    16441 ggaagatata aaacgaaatt tggaaagtgt aaaaaaggca ggagaaaggc ttgtacttac
    16501 tcaaaactat ggcgacatta gtatatataa aaatgcagta aaagaatacc taaaatctgt
    16561 tgtagataat atgtattcac ttgataaaaa tagtagcttt tgggagcatc agtattataa
    16621 gaatgtaaag attgttgata gtaaacttga ggatatgact agtaagttaa ttagtgaaga
    16681 aaaagaaaac atagatattg tttctactgt tgatatgata caaggcttgt tgatagatat
    16741 gtatagataa ggttatacaa ggagaaaagt tttatgaata gtaaaaaaga ggatatatat
    16801 gaattgaatt ttgggcaatt aatagaagaa aaatctggtg ttatagaaga aaatcaaaat
    16861 gtttttaatg caaaagtaaa tgtagctgtt tctataggaa atacaacaga aacaataaaa
    16921 aatatattag atttaaaaga aggctctata ataaaactaa gtaagaatgt ggatgaaaaa
    16981 ttagatatat atgcaaatga tagattgttt gcttatggag aaagcattat tacaaatgag
    17041 aagttatctg taagattatc taaaatagaa gattcagatg taaattagta ttttaaatag
    17101 tatcccttta ggctatgatg tatgagttta gctaagggga tatttttata aaaaatttaa
    17161 aatatgcgtg aattatataa atgtttttat tttatgagcg ataattaaat tataagtatg
    17221 taattttgga ggtgaaacta tggatattaa aaatgtaagt tcaaatattg tagtgaataa
    17281 ttatttaaag aatacatctg gtgtaaaaaa agtggaagaa gttaaaaaaa ttaataaaac
    17341 agacaagata gagctatcta aatttgcaaa ggaaatggca agtgtgtcaa atatgagtga
    17401 tgagaatatt aggaataaaa aagtcgatga aataaaaaat gcattagaaa atggaactta
    17461 caaaataaaa ccaaaagaaa ttgcgaaaaa aatgatagag gatatgcgag gataaagtca
    17521 tgattacaga cttaaaagtt ataatatatg aagaaaaaaa tataattagt aagatgatgg
    17581 aattattaaa agaacaattt gattatatag ttgaaaaaga cttagaaaat ttaaatatgt
    17641 taaatcctaa actagaagat attagcaggg agcttgcatc catagaaata aagagaagac
    17701 aactttttgg tgatgatatt tcaatgagtg aagttgttga aaatagtaat gatagatatt
    17761 taaaagaaat atatacagac ttgaaacaca ttcttgcttt agctaaaaat caacaagaat
    17821 caaatgattc tctaataaag aaagaactta tttttacgaa aaaaatgatt aatttcataa
    17881 aaccagctga taagcaaact gcaacttata attcttatgg aaatattaga aaataaatat
    17941 taggaggaga aatatggcag gattattttc cattttaaat acagctaatt caggaatgaa
    18001 tgcgcaccaa aaagcaatac agacaatttc tcataatata agtaatctag atacagacgg
    18061 ttattctagg caaaggactg agtttgctac aaatagtcca atgtatatgc ctagtttgag
    18121 taattcaatt gcaagaggac aattagggac tggagtacac gtaacggatg taacaagagc
    18181 tagaaatagt ttttatgatt accaatttag agcagaagct cataagtatg gtaatacagt
    18241 ttcaaagtat gactattata atactataga aactataata aatgaaccat cagactatgg
    18301 aatatctgca ggtatagacg acttttttaa aggatgggaa tcattagcaa aggatataaa
    18361 tagtgatagt aaaaaaagtc tagtggtaga aaatgcatca aatctagcaa ctttaataag
    18421 tgaaagttat aagaagctta ataagttaaa gggagatata gacgataata taaactctga
    18481 aataaaacag ataaatgaca tgttaaaaag tcttgaagat ttgaataaat ctattgatgt
    18541 tatatctggt tctggctcta ctccaaatga tttacttgat gaaagagata gaatattaga
    18601 tgacttaagc tttaaattag accttgaaaa tagcgatgtt aaaaatatgc tttcagatgg
    18661 taaattagaa cttaatgaat taaaaaatgc agatggaact tggaaaacag gtgtatcagg
    18721 aaccttacaa ggcttgtttg aaatgcatgg taagattgat acatataaaa gcgatttaaa
    18781 agatgtatct gatgggttag ctaagcagat aaatgatgtt tataatacat ctgctggaat
    18841 aacagtaaca gattttttta taacttcaaa tgtagatggt gaagatatca taaaggtaaa
    18901 tccagctata aagagtaacc caaatgaatt aaaacttact gctgatgagg cttcaaagat
    18961 tgctaagtta aaggatgaaa aaatagatat tggggtggct ggtggaaaag taagtacaat
    19021 aagtgaccac tataaagcct ttgcagaatc agtaggttta gattctcaaa aagtgaatca
    19081 agatgaagtg aatcaacgta aaattataaa taatattgat aactcacgaa tgagtgtttc
    19141 aggcgtatct ttggatgaag aaatgacaga gcttataaaa atacaacgct cctatcaagc
    19201 tagtgcaaaa gttatgtcaa cagctgtgca actgttggat gttgtaataa atggaataat
    19261 ttaatagaag ggggaattta taatgagagt tagtacaggt atgatgtcta gcaactatct
    19321 aaactcacta caagataatc tacaaagatt agataaggtt aatagacaag ttaatacaac
    19381 gaaagaaata aacaagctat cagataatcc atataaggca ataaaaatat tgaactcaaa
    19441 aagtgaaata aaaactatgg agacttatgt tgagaattgt aaagatacag ggaattggtt
    19501 agaaactact gatactgcat tagatcagct tggaaaatta ttatcggaca taaagaaaac
    19561 tttggtggca agtggtgatg gtgcatattc agatgatgag ctaaagactc ttagtaatga
    19621 aactaatgaa aagatgaaag aaattgcaaa tgccttaaat gcaacacatg aaggaaagta
    19681 catattttca ggttcaaata caggtgtacc acctgttgaa tgtatagata atgctgacgg
    19741 ttcagtgagc ttaaagttta attctagttt aaatcttaat aaattaaatg actctttgag
    19801 tatggatata gctcaaggaa taagtgtaga ttataatgtt aaattgagta gccttggatt
    19861 tgacccaaca aagactgacc catttgaaga gttaaataat atatcaaaga aattagcaaa
    19921 tccagatgct gatgctataa aagagctgac aacaacttgt ttaggcgaca tggaacaatt
    19981 aatagaaaat acagtaaatg ttaggtctat atatgggaca aaagcaaata cagtagaggc
    20041 tatgaaggaa aaaaatgatg atagtttgat acagttaaag gatattttgt cacaaaatga
    20101 agaaatagat tatggagaaa aactcgttca attaaaggca gcagagttaa cttatcaagc
    20161 ttcactacaa acaggaggaa agttatttaa tgtatctata ttagattaca tatagtaaaa
    20221 taacgcagat atgatgaagg ttacattaaa aaaaggtata ttaggttttg aaaatttaaa
    20281 agaatatgag cttttagata tagaagataa tgattcttta aaagaattta attctactga
    20341 agaagaaggt ataggatttg ttgttgtatc tccatttgag attatcaatg aatatgaaat
    20401 agttttaaat caggaaacaa tagaaaaact agaagtgaaa tctccaaagg atgttgtact
    20461 atttaatata attactcttg gtcagacatt tgaagaatca actgtgaaca tgaaagctcc
    20521 tatagtgata aatataaaaa ctaattttgg aatgcaaata attttacagg atgataaata
    20581 ttcaataagg catccattat tgagaggtga taatggatgc tagtaatttc aagaaaaaaa
    20641 gatgaagcag tacttatagg agaaagcata gaagtaaaaa ttgtaggggt agatggaaac
    20701 aatgtgaaac tagctatatc ggcgcctaac aatataagta tattaagaaa agaaatatac
    20761 gaaaaagtaa agagtgagaa tataaaagct acaaataaaa atataaaaat tttaaagtca
    20821 ttaaaataat aagcttggag gaagtaatat atgtatggag aaaatcctta taattcttat
    20881 aaacaaaatg ctgtttttat ggcatcaaag gaacaattgt tacttatgct tgttgatgga
    20941 gcagtaaaat ataccaaaat agccagagca gctatcttag ataaaaatac acagaaagcg
    21001 catagagaac ttgtaagagt tcaggatata tttacagaac taatggttac attagaccaa
    21061 aatgcaggac aatgggcaaa ggatatgtac aaagtttatg attttgtaag atatgaactt
    21121 tcaagagcca atattagaaa agacatccag gtaatagata atgtgttgcc tgtaatagaa
    21181 caaattaaag acacatggca tgaagctgat aaaaaaagta aagaagaaag aagtaggtac
    21241 aaataatgga attaaattta gctgaattag ataagttatc aaaagaggaa ttactattga
    21301 tgcttgtaga tggcacagta aaatatacca atatatccaa agaagctctt ttaaataacg
    21361 actatttgaa agcacataat gaacttataa gagttcaaaa tatatttaca gaactaatga
    21421 ctacattaga ccaagatgca ggtcaatggg caaaggatat gtataaagtt tatgaattta
    21481 taaaatctga attagcaata gctgatgaaa ataaagatat taaaataatt gatgatatat
    21541 tgccaatagt aaaacaaatt agagatacat ggcatgaagt ttacaatcaa ctaaaaatat
    21601 aattagatgg agggataaag gtatgtcaag tataagtcct ataagagtta caggtctttc
    21661 aggaaatttt gatatggaag gcataatcga agctagtatg attagagaca aggaaaaagt
    21721 tgataaagca aaacaagaac aacaaatcgt taaatggaag caagaaatat atagaaatgt
    21781 tatacaagaa tcaaaagatc tttatgataa atatctaagc gtaaattctc ctaatagtat
    21841 agtaagtgaa aaagcatact cttctacaag aataaccagt tctgatgaaa gtattatagt
    21901 agcaaaaggc tcagctggtg cagaaaaaat aaattatcaa tttgcagttt ctcaaatggc
    21961 tgaaccagca aaatttacta ttaaattaaa ttcaagtgaa cctattgttc gacagttccc
    22021 tccaaatgcc agtggagcta gttctttaac tataggagat gtaaatatac caatatctga
    22081 acaagatact acaagtacta ttgtaagtaa gataaactcc ctttgcgcag ataatgatat
    22141 aaaggcttct tatagtgaga tgacaggtga attgattatt tcgagaaaac aaactggttc
    22201 gtcatcagac attaatttaa aagtaattgg aaatgacaat ttagctcagc aaattgctaa
    22261 tgataatggt atcacatttg caaatgatgc tagtggaaac aaagtggcaa gtgtatatgg
    22321 aaaaaatcta gaagctgatg taactgatga acatggaaga gtaactcata taagtaaaga
    22381 acaaaattca tttaatatag ataatattga ctataatgta aattcaaaag gaactgcaaa
    22441 gttgacttct gtcactgata ctgaagaagc tgttaaaaat atgcaagcat ttgtggatga
    22501 ttataataaa ctgatggaca aggtctatgg tttagttact actaaaaaac caaaagatta
    22561 tccgcctctt acagatgccc aaaaagaaga tatgacaact gaagaaatag aaaaatggga
    22621 aaagaaagct aaagaaggta tacttagaaa tgatgatgag ttaagaggtt ttgttgaaga
    22681 tattcagtct gcattttttg gagatggaaa aaatattatt gcattaagaa aactaggtat
    22741 caatgaaagc gaaaattaca ataaaaaagg tcaaatatca tttaatgcag atactttttc
    22801 aaaggctctt atagatgata gtgataaggt atacaaaaca ctagcaggtt attcttcgaa
    22861 ttatgatgat aagggaatgt ttgaaaagct aaaagatatt gtatatgaat attctggaag
    22921 ttcaacttct aaacttccta aaaaagcagg tatagaaaaa actgcttctg ctagtgaaaa
    22981 tgtatattca aaacaaattg cagagcaaga aagaaatata agcaggttag ttgaaaaaat
    23041 gaatgataaa gagaaaagac tttatgctaa atattcagcc ttagaatctt tgttgaatca
    23101 gtattcttcc caaatgaatt atttctcaca agcacagggt aattaattat ggataaatta
    23161 aaagaaaaat taaatttata taaagatata tctttacaaa ttataaattt gattgaaaag
    23221 gaagaatata taaagataag tagtaagtta ggtgaaagac aagagattat aaatagtgta
    23281 agtgaaattg atagaaatga ttttatacag ctttataata gaatggaatt aattgaaatt
    23341 gatagtagga taagagatat tttacaagga caattattag aagtaaaaaa agaattacat
    23401 gagtataagc ttactaaaca agtgaacaca atgtattata acttaaatag agaaaaagta
    23461 aatattttta ataaaaaagt ataaagttat gtaccatctt gtccgattat ataaatgaag
    23521 aattaataaa aaagattaaa agtagaaatg acaaggatgt caactatact aaggagggta
    23581 aaataatgag agttaataca aatgtaagtg ctttgatagc aaataaccaa atgggaagaa
    23641 acgtaaatgc acaaagtaag tctatggaga agttatcttc tggtgttaga attaagagag
    23701 ctgctgatga tgctgctggg cttgctatat ctgagaaaat gagagctcaa ataaaaggtt
    23761 tagaccaagc aggaagaaac gttcaagatg gtatatctgt tgtacaaaca gcagaaggtg
    23821 cattagaaga aactggtaat atactacaaa gaatgagaac tttatcagta caatcttcaa
    23881 atgaaactaa tactgctgaa gaaagacaaa agatagctga tgagttgtta caattaaagg
    23941 atgaagttga aagaatatca agctcaattg agtttaatgg taagaaatta cttgatggaa
    24001 gttctacaga aataagatta caagttggag ctaattttgg aacaaatgtt gcaggaacaa
    24061 ctaacaacaa caatgaaata aaagttgctc tagtaaatac ttcaagtata atgagtaaag
    24121 caggtataac aagttctaca atagcaagct taaatgcaga tggaacttct ggaacagatg
    24181 cagctaaaca aatggtgtct agcttagatg tagctcttaa agaattaaat acatcaagag
    24241 caaaattagg ggcacaacaa aatagactag aatcaactca aaacaactta aataatacta
    24301 tagaaaatgt tacagcagct gaatcaagaa taagagatac agatgttgct tcagaaatgg
    24361 ttaacttatc taaaatgaat atattagttc aagcatcaca gtcaatgctt gctcaagcta
    24421 atcagcaacc acaaggagtt ttacaattat taggataaaa taattttgta tattggatat
    24481 agaagaaagg gtaaggcttt gctttactcc tttctttttt tacaaatata cttttgaaag
    24541 gaaaatatat atgttactta gtatagttat gattgttaaa aatgaagaaa aaatacttga
    24601 aaaaacttta aaatcattaa ccacattaag aagttctata gagagtgaac ttataatagt
    24661 tgatacaggt tctacagatg atactataaa tatatctagg aaatacacgg aaaatgtgta
    24721 ctttcataat tggaataatg atttttctag tatgagaaat atatcaatat cctatgctaa
    24781 aggtgaatgg ttacttatat tagatgcaga tgaaatacta attgattctt caactattgt
    24841 gaaatttttt aaagatggtc ttgataaaaa atttaattcg gcctcaatac aattaaaaaa
    24901 tctttactca tatgataaaa aattatatgg atattgctca gtgcttagac tttttaaaaa
    24961 catcgatttt agataccatg gtaaagtaca tgaacaacca atatacaaaa accctatatt
    25021 taataatgtt gctgattttg aacattatgg atatttgttt gaagatgaag aaataagatt
    25081 aaataaagta aaaagaaatg aaaaactgtt atttgaagaa ttaaaagaga atgataattc
    25141 tccatataca aattatcaat tagggaaaaa ttttattata ttaggaaaat atcatgaggc
    25201 tttatactat cttgagaaat cttatgaatt atattctaaa ctagaatcgg cacctggtta
    25261 cttgataaca agtcttgtaa aaacatattt atatttagga aagcataaga agtgtgaaaa
    25321 attgtgtcta aagtatatta agaaagacat gaataatatt gatatatatt attatattgc
    25381 acaagcacaa gtagatttag ggaagtatga aaatagtata gattcgtata agagatatat
    25441 ttatttgtta gataattatg aaataagtac acaagcaaat agtttatttt cagacactga
    25501 tactgtaggg cttagggatg aagctattat tacactgata aagatttatt ataaacttga
    25561 aaaatatgag tctgtaattt cagagtacaa taacattgag gatatggaaa agaagaagaa
    25621 tgtgtacttt agcttattta tgtccctata caaattgaac agatttgaag atataaaaaa
    25681 ctattataaa gaagtttcaa attctaaaat tgaaaaaaat agtttttata aaaatataga
    25741 agaagttata aataatatta aagatgatga aaaagagcat gtatataaaa tcctttgtga
    25801 cattgaaggt aattatggca ttttaaatca gataaggata aataaatgtg tatctataga
    25861 taagtgtaaa gaaatactca atgaagaaaa agaaataatt tatgcatata ttataaaagt
    25921 agcttttgaa gataagattg atttactaga tattttttat gaaatggaat atgtatggat
    25981 agagaaatac ctaaaatatc ttttagcact agaaagaagt tttaatttag tactatataa
    26041 gtacataatt agtcgtccga atacatcaga tataaaaaag ataagagtat ataaagctat
    26101 atctaaagta gttttagaaa gtataagttt aagtgatgaa aaatataagg agatttttta
    26161 tctttatata atgtattctt ataagtacat aagatacata tatagaaatt taagcgattt
    26221 tgaattaata aaatttgttt gcaatgattt agataagttt actatagaat ttaagcatct
    26281 acttgacatt aaaaataaag taaatgatgc taatgaaaaa ttagagtata tacatagtat
    26341 gaggaaatta ctaaatgagt atccttttta taataaaata ataaaaatac ttataagtga
    26401 attagaagaa aatataaaag aaagtgaaga atttaaggat ttaaaagata gttttattat
    26461 tagtatagaa aatatgattg aaactggcaa aataaatgat gcgaaatcat tggtagatga
    26521 ctattcaaaa acatttatgg atgatgtaaa aattttaaat ataaagggaa ttttatatat
    26581 gtttgaaaat aagcttgata aagctgattt tatgttaaaa aaggcattat cattagatgt
    26641 agaaaatgaa gatactatat ataatataga atacttaaaa agtttaagat aagtcaaaaa
    26701 tgggagaatt gaagaatgaa tacaccaata gtggttaatg aaaaatctaa agatataata
    26761 aaagattggc aatatacgta tacaaacgaa gatataaaat ttacaagcat aataatattg
    26821 acatataatc aaatagaata tactaaatta tgtattgaaa gtattaggaa gtttacccca
    26881 aagaataaat atgaaataat aataattgac aataactctt ctgatggaac agttgagtgg
    26941 ttaaagggac aatctgacat aaaatctgta tataataaca aaaacctagg atttccaaaa
    27001 ggatgcaatc aaggtattga gttggctact ggtgaaaaca tattgttgtt aaataatgat
    27061 gtaatagtta ctccaaattg gctttataat ttgaataaag ctttatggtc tcaagataat
    27121 ataggtgctg taggtcctat tacaaatgca tgttcgtact atcaacaaat tgatgttgat
    27181 tataaatcaa ttgaggatat gtttatgttt gcacacaaaa taaatacatc taatattaac
    27241 ctatggaaat atagattaaa gttagttgga ttttgcatgt taataaaaaa atctgtgctg
    27301 gataatgtag gggttttaga tgaattattc acacctggaa attttgaaga tgatgattta
    27361 tcttttagga tgctagagtc aggatataag ctattattat gccaggatac ttttatacac
    27421 cattttggaa gtgtatcttt taaagaaaat cgtaataaat atacagatat aatgtatatt
    27481 aatagaagaa aatttgaaac taagtgggga tttaatccag aatatagtgg aatgataaga
    27541 acagatttga tttctaaaat agatgatagt ttggaggcaa atattaatgt tttagaaata
    27601 ggctgtggta taggggctac tcttttagaa ataaaaaata tatataaaaa tgcatcaata
    27661 tatggaatag aaattagtga gtcagcaggt aaacttgcaa agtatagtgc agatgtactt
    27721 atagcagata tagaaaaagt aaagttggat tatgaaaaag atttttttga ttatataata
    27781 ttgggagatg tacttgaaca tttaactgac ccatggaaga tagtcaatga actgaaacaa
    27841 tatctaaaga aggatggttg tttgatatct agtataccaa atattatgca tgtttctgtt
    27901 ttaagaaacc ttgtgttagg taattttaca tatgaagatt caggaatttt agacaagact
    27961 catttaaggt tttttacatt aaatgagata acaaaaatgt ttaatgatag taattacata
    28021 ataaatagtg ttacagctac tagtatgcca ctttcggaaa ttgataatag attcattgat
    28081 acaatgtgta aggaaagctg taaagaaatg agacaacagt acgaatctta tcaatatata
    28141 gtaaaagcca ctaaaagcat agatactaaa agatattcta gtattagtat ggttaattta
    28201 aaatacgctt taatgagaat tgataatgaa ttagatgtag atagtaattt aagttatata
    28261 tttgataatt ataatataac tgaaccatgt tttgttgatg atattgaata tttagttgat
    28321 acgtttgtga taaataaagc aactctatta aatagattag gagttgaggc atttaacaga
    28381 ggtttaggag attttagtat aaacatgttt atgacagtat tagagatgga tagagatgat
    28441 attgattcaa tatataatat aacaagtgta ttatgtgaat tagaagaatt tgatactgct
    28501 tacaacttaa ttaaaaatag tagcaaaaaa gtacagagtg aaagtgacat acaagatata
    28561 ctttatttta ttgaggggaa gagatatgaa taatgataag atatgtttta taacctgtgt
    28621 aaatgatgaa agactttatg aggaatgtct tgtatatata aatagcctag acattcctaa
    28681 aaactttagc atagaaacag tagctttaag aggagccgat tctattgcct ctgcttataa
    28741 taaagctatg agagaaacag attctaagta taaggtatac ttacatcaag atgcttttat
    28801 aattaataaa aatttcatat atgacatttt agagatattt aaaaaaaata cagatgtagg
    28861 aatgattggt gtagctggag taaaagatat acctgtgaat ggaatttggt gggattctga
    28921 taaaaaagca gggaaagtat atgatagcca tacaggtaaa atggatattt atgaatttag
    28981 taactttgaa gaatgtttta atgaagttat ggctatagat ggattaatta tgattactca
    29041 atatgatgta ttttggagag aggataagtt tgatgggtgg catttttatg atttgtcaca
    29101 gagtatggaa tttagacaaa ataaatacaa agttattgta ccaaaacaaa ataagccttg
    29161 gtgtatgcat gattctggag tagcaaatat aaataatgga tatgatgaat ataggataaa
    29221 atttgtaaaa gagtacataa atcttgattt atatagagaa aatttttatg aatttgataa
    29281 gaagaaagca agtaccatac ttaagagtaa taagcatgta atggattgta tgaaagagga
    29341 gcttacagaa caattaagat taaagaatta taaggatgtt ttagatattg tttctaaaat
    29401 tgctcatttt tctgttttta atcatactgg cttgtattct tgtgaaaaat gtgagagagc
    29461 attgctagaa tgtgcaaata atttaccaga aatagaaaat acgaaattat ttacaaaaaa
    29521 agatttaaaa agaagaaaag tattacatgt tttaagtgaa ggatattcaa caggagggca
    29581 tacaagacta gttaaaaatt ggattgagat tgacaaagat tctgttcatt cagttgtaac
    29641 tacatggcaa tcagatacac tgccagactg gttattaaaa gcaatagatg atagtggtgg
    29701 cgaagttata tctttggatt taatttcaga tgattattta aaaagagctg tagctttgag
    29761 gaaaatagct tatgactggg ctgatatagt tattttatat gttcatatgt gggatacaat
    29821 acctgttgtt gcttttggag ttgatgggaa aatacctatt ttacatgtta atcactatga
    29881 tagtactttt tggttaggga caagtattgc agatattgtc attgatttta gcgaagctag
    29941 tcaattaatt acattagata aaagaggcat agataaatct gaaatattac caatgccatt
    30001 aaaagaaaaa gaagtatttt caaaatctga agttagaaaa cgatataatt tggaggatga
    30061 tgtgacaata atattgacta ttgctagtga aagaaaatac aatagtgtaa atgatattaa
    30121 ttactttaaa attgctaaaa cattgattga aaatacagat aatacaatta ttattgttgt
    30181 aggagttgat acagagaatc tctattggaa aactttaagt aacgttacag ataatagagt
    30241 tatatgtttt ggaacaacag caaatataga cgatttttat aagatttcgg atatatacct
    30301 agagtctttc atggtaggga gtcatacttc aaaattagat gcaattttat atgatttaat
    30361 tcctgttaag tttaaaaatg aagtttcacc aatttttaca aatatgtgga attttataga
    30421 taagtttgat tataagaata taaaagaggt tatagacatg ataagaaggt acaatactag
    30481 tgaatttgac agggataaga taaaagaaaa acaatcagaa ataaaacatt ttgttataga
    30541 taaacactgt attgaaataa aaaactatat agacaaaata tataaagagg ctaatcatca
    30601 taaagttaag tatgatttat ataaaaatga tgaaatcgaa gactatcagc tttttgttgc
    30661 attatatggt tatatggtaa aaaataaaaa catgtcatag gtgataaaat gaacaatatt
    30721 catatcaata aatttgaaaa aagctgtgaa aaaaactggg attattttgt agaaaataaa
    30781 tctataaatg gtacatttct gcaaactaga aatttcctta attatcatga agaaggaaaa
    30841 ttcactgacc actcattaat aatatataaa ggtgaagaaa tactagcagt cgtacctgca
    30901 tgtgaaactt atgaaaatgg tgaaagagta tttttttctc atttaggaag cacttttgga
    30961 ggtattataa taagaagtgg ttttaatgat attaggcatg taaatgctat tacaaataag
    31021 ttagaaacat atttacagga acaaagtttc aataaagtat atttaaaaaa tacatcaaga
    31081 atattttgta aagaggatac ttcattacta gaatattttc tatttaagaa taattacatg
    31141 ttttatgatg aattaagtat gtttttagaa ttaagtaagt ataaaaaaga tatattagct
    31201 aattttagat atagtaaaag aagatatttt aaatattctt tgaagaataa t
//
LOCUS       DAELXX010000036        30907 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.036, whole genome shotgun sequence.
ACCESSION   DAELXX010000036 DAELXX010000000
VERSION     DAELXX010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30907
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.036"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            27..101
                     /locus_tag="KPH29_001604"
     tRNA            27..101
                     /locus_tag="KPH29_001604"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="KPH29_001605"
     tRNA            116..200
                     /locus_tag="KPH29_001605"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="KPH29_001606"
     tRNA            210..293
                     /locus_tag="KPH29_001606"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            317..391
                     /locus_tag="KPH29_001607"
     tRNA            317..391
                     /locus_tag="KPH29_001607"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:349..351,aa:Gly,seq:gcc)
     gene            416..492
                     /locus_tag="KPH29_001608"
     tRNA            416..492
                     /locus_tag="KPH29_001608"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:450..452,aa:Arg,seq:tct)
     gene            502..577
                     /locus_tag="KPH29_001609"
     tRNA            502..577
                     /locus_tag="KPH29_001609"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:535..537,aa:Gln,seq:ttg)
     gene            586..661
                     /locus_tag="KPH29_001610"
     tRNA            586..661
                     /locus_tag="KPH29_001610"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:619..621,aa:Lys,seq:ttt)
     gene            664..752
                     /locus_tag="KPH29_001611"
     tRNA            664..752
                     /locus_tag="KPH29_001611"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:698..700,aa:Ser,seq:tga)
     gene            756..831
                     /locus_tag="KPH29_001612"
     tRNA            756..831
                     /locus_tag="KPH29_001612"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:789..791,aa:Phe,seq:gaa)
     gene            838..911
                     /locus_tag="KPH29_001613"
     tRNA            838..911
                     /locus_tag="KPH29_001613"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Met,seq:cat)
     gene            926..1002
                     /locus_tag="KPH29_001614"
     tRNA            926..1002
                     /locus_tag="KPH29_001614"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:960..962,aa:Ile,seq:cat)
     gene            1020..1096
                     /locus_tag="KPH29_001615"
     tRNA            1020..1096
                     /locus_tag="KPH29_001615"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1054..1056,aa:His,seq:gtg)
     gene            1105..1180
                     /locus_tag="KPH29_001616"
     tRNA            1105..1180
                     /locus_tag="KPH29_001616"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1138..1140,aa:Lys,seq:ttt)
     gene            1188..1261
                     /locus_tag="KPH29_001617"
     tRNA            1188..1261
                     /locus_tag="KPH29_001617"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1220..1222,aa:Cys,seq:gca)
     gene            1269..1345
                     /locus_tag="KPH29_001618"
     tRNA            1269..1345
                     /locus_tag="KPH29_001618"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1303..1305,aa:Arg,seq:acg)
     gene            1358..1433
                     /locus_tag="KPH29_001619"
     tRNA            1358..1433
                     /locus_tag="KPH29_001619"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1391..1393,aa:Val,seq:tac)
     gene            1509..1922
                     /locus_tag="KPH29_001620"
     CDS             1509..1922
                     /locus_tag="KPH29_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467223.1"
                     /translation="MYIVIGNEIVDSEELKIIIDENSKFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLSSLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVDGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            2231..5662
                     /locus_tag="KPH29_001621"
     CDS             2231..5662
                     /locus_tag="KPH29_001621"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBG1467224.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTKESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            5904..7832
                     /locus_tag="KPH29_001622"
     CDS             5904..7832
                     /locus_tag="KPH29_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBG1467225.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
     gene            8285..8749
                     /locus_tag="KPH29_001623"
     CDS             8285..8749
                     /locus_tag="KPH29_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBG1467226.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            8756..9250
                     /locus_tag="KPH29_001624"
     CDS             8756..9250
                     /locus_tag="KPH29_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HBG1467227.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            9266..10291
                     /locus_tag="KPH29_001625"
     CDS             9266..10291
                     /locus_tag="KPH29_001625"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HBG1467228.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            10278..12725
                     /locus_tag="KPH29_001626"
     CDS             10278..12725
                     /locus_tag="KPH29_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBG1467229.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNRVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            12905..14278
                     /gene="radA"
                     /locus_tag="KPH29_001627"
     CDS             12905..14278
                     /gene="radA"
                     /locus_tag="KPH29_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBG1467230.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDENIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            14281..15351
                     /gene="disA"
                     /locus_tag="KPH29_001628"
     CDS             14281..15351
                     /gene="disA"
                     /locus_tag="KPH29_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HBG1467231.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            15440..16534
                     /locus_tag="KPH29_001629"
     CDS             15440..16534
                     /locus_tag="KPH29_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBG1467232.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            16663..18840
                     /locus_tag="KPH29_001630"
     CDS             16663..18840
                     /locus_tag="KPH29_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467233.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDMSTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSENGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KEAYKNNDVVFVELGDTYRLDLYKPNLNEKTYESVKDNIEKNIDVYLKNIFSMVEEND
                     TVYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWSIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVLFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
     gene            complement(19026..19871)
                     /locus_tag="KPH29_001631"
     CDS             complement(19026..19871)
                     /locus_tag="KPH29_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1467234.1"
                     /translation="MNRNNFSHEIIEPIDNLDVNFKLFDDSGSYVANHWHNSLEIIYI
                     TSGDLQINMEGYTYNLKANECMFINSGIIHSTRCTYQDTSILLQVPLSFLNKYIPDFK
                     NCYFDFKVNANDNNYKKNSGKVKLILENMREIKLSSPPAANLLFTSLLFELLFELYTN
                     FKISVGNKNMKKTVLDLSKFEPVLEYTKINYKMPISINKIAKVAHLQPEYFCRKFKQY
                     MGQTYLEYLNDVRISHIYKDLINTNDTLCSILEAHGFTNTKLFYRIFKEKFKCTPKHI
                     RKKST"
     gene            20088..21254
                     /locus_tag="KPH29_001632"
     CDS             20088..21254
                     /locus_tag="KPH29_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HBG1467235.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFATIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYVAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMARGSKPNSLEFLRDFYIKAYKVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNIVLDTHQYLMLAESDGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYACGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLGKCVAQEWYPIEY"
     gene            21276..22922
                     /locus_tag="KPH29_001633"
     CDS             21276..22922
                     /locus_tag="KPH29_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBG1467236.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYIHSSGFDASILHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWRPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNVLGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYIQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIADESKDVD"
     gene            23019..24392
                     /locus_tag="KPH29_001634"
     CDS             23019..24392
                     /locus_tag="KPH29_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG1467237.1"
                     /translation="MNTEVNVKATTIRYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCGTLLINGLTLPLVEYFGNNLSAWTKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQNYIG
                     VIFASAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMFFYKLDKEYSTI
                     LNELNTK"
     gene            24837..25790
                     /locus_tag="KPH29_001635"
     CDS             24837..25790
                     /locus_tag="KPH29_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HBG1467238.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            25828..26790
                     /locus_tag="KPH29_001636"
     CDS             25828..26790
                     /locus_tag="KPH29_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HBG1467239.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            26815..27801
                     /locus_tag="KPH29_001637"
     CDS             26815..27801
                     /locus_tag="KPH29_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBG1467240.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            27814..28860
                     /locus_tag="KPH29_001638"
     CDS             27814..28860
                     /locus_tag="KPH29_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HBG1467241.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYSGKIRKCDVEKLTAKETIVSNNTSKYSE
                     KKELKIENENSSMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            29027..30757
                     /gene="lpdA"
                     /locus_tag="KPH29_001639"
     CDS             29027..30757
                     /gene="lpdA"
                     /locus_tag="KPH29_001639"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBG1467242.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDIV
                     EENQNIEVKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEVPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
BASE COUNT        12416 a         3406 c         5930 g         9155 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atgtaaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttat
      301 ttaatttaat aaacgagcgg gaatagttca gtggtagagc gcaaccttgc caaggttgaa
      361 gtcgcgagtt cgaatctcgt ttcccgctcc aaattttatg tttatgttgt aatgtgggtg
      421 catagctcag ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct
      481 gtgcgctcac cacatatata ttggggatta gccaagtcgg taaggcacat gactttgact
      541 catgtatgcg taggttcgag tcctgcatcc ccagccaaac agtatggtct attagctcag
      601 tcggtagagc acctgacttt taatcagggt gtcccgcgtt cgagtcgcgg atagatcacc
      661 aatggagagg tgtccgagtg gtttaaggag ctggtcttga aaaccagtga cttcgaaagg
      721 ggccgtgggt tcgaatccca ccctctccgc caaatgccca gatagctcag tcggtagagc
      781 aggggactga aaatccccgt gtcggtggtt cgattccgcc tctgggcacc aaaatgtggc
      841 ggtatagctt agttggctag agcgttcggt tcatacccga aaggtcatag gttcgactcc
      901 tattaccgct actaaaattt aatgtgggcc attagctcag ttggttagag cgcccggctc
      961 ataaccggta ggtctggggt tcgagtccct gatggcccac cacattaaat taacttatgg
     1021 tgggtatagc tcagttggtt agagcgccag attgtggctc tggaggtcgt gagttcgact
     1081 ctcattatcc accccaactt atatggtcta ttagctcagt cggtagagca cctgactttt
     1141 aatcagggtg tcccgcgttc gagtcgcgga tagatcacca aaaaggcggc gacatagcca
     1201 agtggtaagg cagtggactg caactccttg atccccagtt cgaatctggg tgtcgcctcc
     1261 aataaaatgc gctcatagct caactggata gagtgtctga ctacgaatca gaaggttagg
     1321 ggttcgagtc cctttgggcg caccatatga atagcgaggg attatagctc agctgggaga
     1381 gcacctgcct tacaagcagg gggtcacagg ttcgagccct gttaatccca ccagtgcaaa
     1441 cctctagtaa aactactaga ggttttattt ttgattgaaa aagtttttat atttaggagg
     1501 taagaaattt gtatatagta ataggaaatg aaatagttga tagtgaagaa cttaaaataa
     1561 ttatagatga aaatagtaaa tttaaagttg aaaaagattt aagtaaaagc acaaaaagag
     1621 aagatgtaat agcatatcag ttaagtatag atttaaatta cctagattct cttataaatg
     1681 agcaatgtaa tttgtccagt ttatctgatg aagagaaatt tgatgaatat atgactttgt
     1741 ctgatgaact tgcactggat ttagaagagt taatgccaaa atatacaata ataaatgcta
     1801 gagcatataa attagacgag gtagatggaa ttgtaaaaat aatactagct gtagcgtatg
     1861 cagatttagg gcacttaaaa ctttctgatg tagtaaagag gctatctaga caagtagatt
     1921 aggatatgaa tatgaactat attaaaaaaa tgtataaaat cttacgtaaa gatgaaaata
     1981 attatgtaaa catagaaaaa aacagtataa aatcaagtca attgtagaaa ttttcacata
     2041 aattcatctg aaattctata aaaaatgcct taattttaat gaggtaaatc gagtttacac
     2101 agtctaaaaa ttacttatat ttataaattt atcttattaa ttaaggaact ttcttaaaca
     2161 tcttcaaaaa taaagtattt tagtctataa tctttaatat tacacaaaca ataaaggaga
     2221 agaatagagt atgcttaaaa agtttaataa aatactagtg gctaatagag gcgaaatagc
     2281 tataagaata tttagagcct gttcagaatt ggggataaaa agtgttggta tatacagtaa
     2341 ggaagataag tatggattgt ttagaacaaa agcagatgaa tcatatttga taggagaagg
     2401 taaagggcca atagatgcat atttagatat ggatggaata atagacctag caaagagaaa
     2461 aaaagttgat gctatacatc caggatatgg ttttctagcg gagaatgcag aatttgctcg
     2521 taaatgtgaa gagaatggaa ttacttttat aggaccatca tctaaggtaa tgaatatgat
     2581 gggggataag attaactcta aaaaaatcgc aaaagaagtt aatgttcaaa ctataccagg
     2641 agtagaaaaa gcaataagaa gtactgaaga agcaaaggaa gttgcaaaca aaataggata
     2701 tcctgttatg ataaaagcat ctaatggcgg tggcggtaga ggtatgagaa ttgtacatcg
     2761 cgaagaagac ttagaacttg aatatgaaac tgcttgtagt gaatcaagaa aagcttttgg
     2821 agaagatatt atatttatag aaaaatatat agctgatcca aaacatatag aagttcaaat
     2881 attaggtgat aattatggaa atatagttca cttatatgaa agagattgtt cagttcaaag
     2941 aagacatcaa aaaataatag aatatgcacc agctttttcg ttagatgata aagtaagaaa
     3001 agagatatgt gaagatgcag ttaagttatc aaaacatgtg ggttattcta atgcgggaac
     3061 attagaattt ttagtagatg caaatggtgg gcattatttt atagagatga acacaagagt
     3121 acaggttgaa catactgtaa cagagatggt tacaggaata gatatagttc aaagtcaaat
     3181 actaatagcc caaggttaca gcttagatag tgaagaaatt aatataaaat cacaagatga
     3241 tgtggaaata agaggttact ctatccaatg tagaataact acagaagacc ctaagaataa
     3301 attcatgcct gatacaggta aaattcaagt ttatagaact ggttcaggat ttggtataag
     3361 actagatgga ggaaatggat ttacaggggc aaacataagt cctcattatg atagtttatt
     3421 ggttaagaca atatcttggg atagaacatt ccaaggagca ataaataaaa ctataagatc
     3481 tataaaagaa cttagagtta gaggtgttaa aactaatgta ggattcctag ttaatgtttt
     3541 aaataatcct atattctcaa atggtaaatg tagtactaag tttatagatg aaaatccaga
     3601 tttatttgaa ataacagaaa gtaaagacag aggaacaaaa ttacttcaat ttataggaga
     3661 tgtaattgta aatgataatg cttgtaaaga aaaaccacta tttgatgcat tacatgaccc
     3721 aagaatggat aaggatggtt caaaatcaga aggaagtaaa atacttttcg ataagttagg
     3781 taaatctgca tatatagaaa agataaaaaa tgataagaaa ttactattaa cagatacaac
     3841 aatgagagat gcacatcaat cattattagc aacaagaata agaacatatg acttattaaa
     3901 agctgctaag ccaacagaaa aatatcaaaa ggatttattc tcacttgaaa tgtggggagg
     3961 agctacttat gatgttgcat atagattttt aaaagaatct ccttggcgta gattacaaaa
     4021 gctaagagaa gaaataccaa gcataatgtt ccaaatgttg cttagagcat caaatggagt
     4081 tggatataaa aactatccag ataatgtaat agaagaattt acaaaagaat ctgctagaca
     4141 aggtatagat gtatttagaa tatttgattc attaaactgg gttgaaaata tgaaaccatc
     4201 tattaataca gctttagaaa ctggaaaaat tgttgaagca actatgtgtt atacaggtga
     4261 tatactagat aagactaaaa ctaaatacaa tctagaatat tatattaaaa tggcacaaga
     4321 attagaaagc ttgggagcag atataatagc aataaaagat atgtcaggtc ttcttaaacc
     4381 atattcagct tacacactag ttaaagagtt aaagaaaaat gtaaaagcac caatacattt
     4441 acacactcat gatacaagtg gaaatggtgt agcaacttgt ttaatggcgt cagaagctgg
     4501 agtagacata attgatgctg cattagaatc tatggcagga cttacaagtc agccatcgct
     4561 taatgctata gtagaggcac ttaagaatac agagagagat acaggaatag atttatttgg
     4621 atatgatgag ttaggaaaat attataagga tttaagaaaa gtttataata aatttgaaag
     4681 tgatttaact aattcttgtg cagaaatata taattttgaa ataccaggag gccaatatac
     4741 aaacttaaaa cctcaagctg atagtttagg attagttaat agatttgatg aagttaaaga
     4801 aaaatataaa gaagctaatg aagttgtagg agatataata aaagtaactc catcttcaaa
     4861 agttgtagga gacttagcta tctttatgac taaaaataaa ttagataaag acaatataat
     4921 agaagaaggt aaaaatttat cattcccaga ttctgttgtg gattactgta aaggtatgat
     4981 tggtcaacca gaaggtggaa taccaaaaga tttacaagag gtagttctaa aaggtgaaga
     5041 agctataaca gtaagaccag gttcattatt accagcagaa gattttgatg aaatagctaa
     5101 atatctaaat gaaaagtatg atataaatgc taatataaga aatgttataa gttatgcttt
     5161 atatccaaaa gtatatgaag attatatcaa acatctacaa cattataatg atatatctaa
     5221 acttgaaagt gatgtattct tctatggatt aaataaaaat gaagaatgtg aagtagaaat
     5281 agaagaaggt aaggtattaa caataagatt agttgaaatt ggtgaagtta aggaaaatgg
     5341 atttagaact ataggatttg agttaaatgg aatggttaga gaagtagaaa taaaagataa
     5401 gaatttctct ggaaaaatta ataatgttga aaaagctgat atgaatgacc ctcttcaaat
     5461 tggagcaagc ataccaggaa aagttataaa aataatggtt aaagaagaag atgaagtaaa
     5521 agcaaatcaa ccattaatcg ttatagaggc tatgaagatg gaaactataa tagttgctaa
     5581 gactgatgga gtaattaagt caatcaaagt aaaagaagat gatatggttg aagataaaca
     5641 attattaatg ataatgaagt aatttaatta aaataaatta aaaagcactc tgaaatgttt
     5701 aataaaaaat gttttagagt gtttttattt attttaatta aatatttgtt aaaaaaaaga
     5761 ggacaattac ctccagatgt agaaataaag ctagtaagga aaatgttatc taaaaattct
     5821 aaatatagta aatattttcc ataatatttc ttaggaaata acagcaaaga taaaatacat
     5881 aaatttaaaa ggtgggtgct tatatgaagg tttatgatag taaaatgttg agaaatgtag
     5941 cagtattagg acatagcgga tgtggaaaaa caaatctaat cgaaactata gcttacacag
     6001 caaacactaa taagatacct aaattaacag acaaggtaaa tatgacttat agcatgggat
     6061 taatacctat agagtataat gattataagt ttaacttatt ggatacccca ggatattttg
     6121 actttagcgg agacgttgtg tcatctctta gagcaagtga tgcagcaata atagtaatag
     6181 atgcaacagc gcctattcaa gttggtacag agaaatcatt agaactaact gagagtattc
     6241 ctaagataat gtttataaat aaaatagata atgaaaaggc aagatacaaa gatgcaatag
     6301 caatgttaag agaaaaatac aataataaga tagttcctat gataagtcct atatacaaag
     6361 ataaaaactt tgtaaagtta cataatgttt ttgaaaacat agatgattta gaaggtgaat
     6421 ttaaggaaca agctatgagt gttaaagaag cattaatgga acttatagca gaaactgatg
     6481 accaaatatt agataaatat tttaatggag aagaactaac tacggaagag atacaaaaag
     6541 gtattattat aggtatacaa agaggtgata ttatacctgt aatatgtggt tcaactataa
     6601 ataatatagg cacaaaggaa atattagata caatatcaag ttatttagaa cctatcttta
     6661 cagaggaaag caaaccattt aggggattag tatttaaaac tatggttgac ccatttgttg
     6721 gaaagatgtc atatataaag attactgagg gtgtactttc taaggataaa gatgttttta
     6781 atataaataa aaatgtaaaa gaaaaaatag ctaatatata tacattgaga aatagtgaat
     6841 tagtagaaat agaaaaagct aaagctggtg atatagtagt tataactaaa gttaattctc
     6901 taaaaacagg agacactata tctgcagata aagatgcaga agctttagaa aagatagatt
     6961 ttcctaaacc acaaatatat tatgcagtaa ctcctaagaa taaaggtgat gaagaaaaag
     7021 tagcatctgt attaaataaa ctagtagaag aggaccctac attacattgg tatagaaata
     7081 cagagacaaa acaagcactt ttaggaggtc aaggagaact tcatataaaa acaattaaaa
     7141 ataaaatgaa agataagttt ggtgttgatg ttgaattgaa tgaccttaaa gttccttata
     7201 gagaaactat aaaaggaaca gccgatgtac aaggaaaaca taaaaagcaa tcaggtggtc
     7261 atggtcaata tggagacgta aaaataagat ttgaaagatg tgaatctgat tttgaattta
     7321 cagaagaaat atttggtggt tctgtaccaa aacaatatat accagcagtt gaaaaaggtc
     7381 taaaagactc tatgcaaaaa ggtatattag caggttatcc tgttacaaac ataaaagcta
     7441 cgctttatga tggttcatac catgatgtag actcttctga aatggccttt aagatggcag
     7501 caagtgcagc ctttaaaaaa ggtatggaag aagcgcaccc aatactacta gaacctataa
     7561 tgaaacttaa gataactgtt ccagaagaat atatgggaga tgtcatgggt gatataaata
     7621 agagaagagg aaaaatattt ggaatggaac cagatgacaa agggaaacaa ataatatttg
     7681 cagaagctcc acaagcagag acatttaaat atgcaataga tttaagagca atgacacaag
     7741 gtagaggata ctttgaaatg gaattagaga gatatggaga ggttccatca cagtttgcag
     7801 aaaaaataat tgggttagct actgcaaagt aatgaacaaa ataaatagaa aaataattaa
     7861 tatatattat taaaaatatt tttagggttt taactaggta taggtttatg ttaggtaaac
     7921 ttatacctag tttttataca tttttgatga ataaaaaaat atataatact ttcttaaatg
     7981 ttttaataaa aagaaagtat ttttttatta ttatttttac aaatgtttag taataaagat
     8041 gagaaggtat atttaaaata taaagtattt aaagttctga aatctaatca tgaagcaaaa
     8101 aattagcatt tggttatcaa taaatataat aaataaaaac tcataattaa tgaaagaata
     8161 aattcatatc taaatttcta taaaaaattt aaatttatac aatttagggc tttaaaaatt
     8221 taaataatag gtgtaaaata atagtcaaag aaagtcaaaa ataaaaaggt gtgtggtgat
     8281 attaatggca actatgacag atataattga aaaatttatt aaagatttga tggaagaaga
     8341 taatagcata caaattcaaa gaaatgaact agcaaacttg tttagttgtg caccatcaca
     8401 aataaattat gtcttaacaa caagatttac aatagataga ggatattaca ttgaaagtaa
     8461 aaaaggtggc ggaggatatg ttcaaataga aaaaatacga aaaagtaaag atggacatat
     8521 tagagaacta ctaaatgaaa aaataggaag tcagatatcc tataaaaaag caaaggaatt
     8581 attagaaggt ttaaaagagt cggatttgat taacgaaaga gaattaaaat taatattata
     8641 tgcaatagat gataaatcat tgtgtatgcc aatatatgaa ttaaaagaga aggtaaggtc
     8701 taatatttta aagaatataa taataggatt atttagtata gaggagtgat aatttatgct
     8761 atgtcaaaaa tgcaataaga ataaagcatc tgtatattat aataagatag tcaatggaga
     8821 aaaaacagag atgtatttat gtagtgagtg tgcaaaagag aatacagaaa tgaattttaa
     8881 cttagatatg cctttttcaa tgatggatat attttctaat ttaggttttc aacctaaaaa
     8941 agaacttgag gaaaagctag tttgtcctaa atgcaataca acatatagtg agtttaagaa
     9001 taatgggaga tttggatgta gtgaatgtta taatgcattt agtagtcaag taaatccaat
     9061 gcttcaaaat atacatgggc atatagaaca tacaggaaaa gcacctaaaa aatcttttta
     9121 taagataagt gtagaaaatg agattaaaga actaaaagaa gatttagata gagctataaa
     9181 aaatgaagag tatgaattag cagctcaatt tagagataaa attaaatatt taaaaggttc
     9241 tattgactaa gattggagtg atattatgaa agagaacata gtcatgaaat caagagttag
     9301 gctagctaga aatttaaata actatccatt tccaaataaa ttagataaag aatgtgccat
     9361 ggaaataata gaaaaagtaa aaaatgcgtt tattaattct aacttagaac aaaaagaaga
     9421 atttgatttc tataaaattg aagatttaga ccaatctaaa aaaatgctta tggttgaaga
     9481 acatataata agcccagatt tagcagaaaa tgataaaagt gcagttatag taaaaaaaga
     9541 taaaactata agcataatga taaatgaaga agaccatata aggatacaaa ctatatgtga
     9601 tgatttaaac ttagaatatg catatagtgt agcaaatgaa atagatgatt tgttagaatc
     9661 aagtttagaa tatgccttta acacaaagct aggttatcta acatcttgtc caactaatac
     9721 aggaacagga atgagagctt ctgtaatgat gcatttacca gcattaagtc aactaggcta
     9781 tatggatgaa ctttataaaa tttcatcaca aataggtata gcaattagag ggatatatgg
     9841 agaacgaaca gaagcattgg gaaatatata tcaaatatct aatcaactta cacttggaag
     9901 aacagagtct aatattatag aaaatgtaag tggacttaca aaagatgcaa tttcaaaaga
     9961 aataaaagct agagaaatat tgcaaaaaaa attaggtata aaactagaag ataaaatatt
    10021 tagatcgata gggacacttg aaaattcaag agtaatgagt agcgcagagg caatgagcca
    10081 tctatcaaat ataaaaatgg gaatagaaat gaactatata gataaactag atttaagggc
    10141 tatagaacaa cttatgatag gtatacaacc agcgcatcag tcaataatgt ataagagtga
    10201 tgatgttgag aatagagata taaatagagc aacatatatt agagaaactt tggaaaaatt
    10261 aagaggaggg atgaattgtg aacttcaata gatttacaca aagagctaaa aaggcaattg
    10321 atttagcgtt tgaatctgct aaaagtctag gacacaatat tgttggaagt gaacatatac
    10381 ttttaggtct tttaagagaa gaagaaggta tagctgctaa agttctaagt aaagttggat
    10441 ttacagaagc ttatttagaa ggcaaaatag ttgatatgga aggtaaggga gaagaaatct
    10501 cagaagatat agtattaagt ccaaggagta aacaaatact tgaattatca ggaatgtttg
    10561 caaataaatt aaaaacaaat tatattggga ctgagcatat attattagca attattcaag
    10621 aaggtgaagg aatagctaat aagattttaa attatgctgg agtaaatgat aggactttag
    10681 cccaattaac aattgatatg atgggtatta gtgataaaaa tcaatacaaa gcagagaata
    10741 gttacacggg caaccagaat caagcagaat ctaaagtact tgataagtat ggaagaaatc
    10801 ttacactata tgcaaaacag aataaaatag accctgtaat aggtagagaa aaagaaatac
    10861 aaagagtaat acaaatatta agtagaagaa ctaaaaacaa tccagtgttg ataggagacc
    10921 caggtgtagg gaaaacagcc atagctgaag gtctggctac aaatatagcc ttaggaaatg
    10981 ttccagaaac actaaaaaac aaaactttat actctttaga gatgggttca ttattggctg
    11041 gggctaagta tagaggagaa tttgaagaaa gaattaaaga agttgtagat gaagttgtta
    11101 aaaatggaaa cataatctta tttatagatg agatgcacac aataataggg gctgggtcta
    11161 ctggagaagg ctctatagat gcttcaaata tattaaagcc agccttggca agaggagaaa
    11221 tacaagttat aggtgcaact acaattgatg aatatagaaa gcatgttgaa aaagattctg
    11281 ctctagaaag aagattccaa ccagttatgg tagatgaacc aactaaagag gactcaataa
    11341 aaatattaga aggattaaga gataaatatg aagctcatca taaagttaaa ataactgatg
    11401 atgctataaa ggcagctgta gaattatcaa ctagatatat atcagacaga tatctgccag
    11461 ataaagctat agacttgatt gatgaagctg catcaaaagt aagattaaaa gaaaatacac
    11521 ctccttcaga aataaaaaaa ttagaactag aaatagaaaa tatagataaa gaaaaagaag
    11581 aagcagtaag atgtcaagac tttgagaaag cagccaaaat aagagatgaa caaggtctac
    11641 ttaaaaaaca attagaagat gttagagaaa gatggaacaa gtcatctaaa cattcagatt
    11701 tagttgatgg agaagttatt gctgaagtag tgggcttatg gacaggaata ccagttaata
    11761 aaattcttga agaagaggct gatagacttt tgagacttga ggaaatactg cacaatagag
    11821 ttataggtca agaacaagca gtaaaatcta tttcaaaggc aatcagaagg tcaagagcag
    11881 gtcttaaaga ccctaataga ccaattggtt catttttatt tttaggtcca acaggagtag
    11941 gtaaaacaga attatctaag gcattagcag aagtgcaatt tggagatgaa aatcaaataa
    12001 ttagaataga tatgtctgaa tatatggaaa aacatgctgt gtcaagaatg ataggttcac
    12061 ctccaggtta tgtaggtcat gatgaaggtg gtcaattaac tgaaaaagta agaagaaatc
    12121 catattcagt tatcttattt gatgagatag aaaaagctca tccagatgta tttaatattc
    12181 tgttacaaat tctagatgat ggtagactaa ctgattcaaa aggaagaact gtagatttta
    12241 agaatactat tgtaataatg acatcaaatg ttggagcatc tacaattggt agacaaaaaa
    12301 ctttaggatt tagtatagct aaaggagacg aagaagaaaa atctcaatat gaaaaaatga
    12361 aagaaaatat aatgggtgaa ttaaaacaaa gattcagacc agagttttta aacagaatag
    12421 atgatataat tgttttccac tcactaaatg aaaatcatat atcaaaaata gtacttttaa
    12481 tggcagcgaa actacaagag agattaaaag aaatggatat aaaattagaa atgagtgatg
    12541 aggctgttaa attaatttct aaatctggat ttgacttaga atatggagca agacctctta
    12601 aaagagcttt acaaaaagag ttggaagatg agctatctga agcaatctta aaaggtaatg
    12661 tgaaaaaggg aagtaatgta gtagcaaaag tcaaagatga aaaaatagtt tttgaaacta
    12721 aataatttaa tagagttgca ttttaaaata ttgcaaatat aagttttata atctaaacaa
    12781 aattttaagg gcaatgaatc tataataatt cattgtcctt aattatttta tttaatcttt
    12841 acatattgtc tttcagatat gctaaaatta tagtatagaa taaaaaatta aggatgataa
    12901 aagtatggca aaaataaaaa caaaatatgt atgtcaatct tgtggatatg aaacagctaa
    12961 atggcttggt aaatgtccag aatgtacaaa atggaataca tttgtagaag aaatagacca
    13021 aaaaagcacc aaaaaagagg tctttataat agataaatct tcatctaaac cagtcagtat
    13081 aaactcaata gaaagtaaag aagaagagag gtttacaaca gatataaatg aattagatag
    13141 agtgcttgga ggaggtatag ttaaaggctc tttagtgctt gttggtggtg accctggtat
    13201 aggtaaatca acacttttaa tccaagtatc tagtaatgtt gccaatttag gaaaaactgt
    13261 tttatatata actggagaag aatcagaatc tcaaataaaa atgagagcta aaagattagg
    13321 tattaactca gaaaatttat atatatttgc tgaaaataat ttaagtataa ttgaatctta
    13381 tttagaaagt gtaaatccag aattaataat tctagactca attcaaactg tcttcagtcc
    13441 agaaatatcc tcggcaccag gaactgtcag ccaaattaaa gaaggtactt caaaattcat
    13501 gaaaatttct aaaaaaatgg gaatttcaac gttcatagtt gggcatgtta caaaagaagg
    13561 ttcactagca ggaccaaaat tattagagca tatggttgat actgttctat attttgaagg
    13621 agaaagatat aatacttata gattagtaag agctgttaaa aatcgatttg gttcaactaa
    13681 tgaacttgga gtatttgaaa tgagggattt aggattagta gaacttgata atccatctaa
    13741 aattttaatc tctgaaaaac caaaagatgt agcaggttca gtaataatat ctactgtaga
    13801 aggtacgaga ccaatgttac ttgaattgca agcattagtt tctcctacaa gttttggtat
    13861 accaaaaaga acatctacag gagtcgatta caatagagta ggtatgttat tagcagtatt
    13921 agaaaaacgt gttggtttgc aaatacaaaa tcaagatgtc tatataaaca tagttggagg
    13981 aataaagata aatgaaccct caattgattt agggatagcc atttctgtag catctagttt
    14041 tagaaatata ccgatagatg aaaatatagc tgttacagga gaagtgggac ttacaggaga
    14101 agttagagca gtaagcttta tagaaaagag aatagctgag tgtaaaaaac ttggatttaa
    14161 aaagatagta gttcctagaa gtaattatga tgttgtaaaa gagaccaaag gcatagaaat
    14221 atggccagta gataacttaa gacaagctat aaatatagta cttgggagga atcaataaat
    14281 atggagaatt ttctagataa taaaaatatg ctatatgcat taaaaatgat atctcctgga
    14341 actccactta gattaggtct aaacaatgta ctaagagcta agactggtgg attaattgta
    14401 attgcaacaa acgaagatgt aatgaaaata gtagatggag gatttgctat aaatgcagaa
    14461 tattcaccat catatctata tgaattagct aaaatggatg gagctatagt tttaagtggt
    14521 gatgtaaaga aaatattatt tgctaatgca caacttatac ctgactattt tatagaaaca
    14581 tcagagacag gaacaagaca tagaacagca gaaagagtag caaaacaaac tggtgctata
    14641 gtcataggaa tttcacaaag aagaaatgtt ataacagttt atagaggaaa tgagaagtat
    14701 gtagtcgaag atatatctaa gatatttact aaggcaaatc aggctataca aactctggaa
    14761 aaatataaga cagtattgga ccaagctgta acaaatttaa atgccttaga gtttaatgat
    14821 ttggtaacta tttatgatgt tgcattagtc atgcaaaaga tggaaatggt aatgagagtt
    14881 acaagtataa ttgaaaaata tgtgatagaa ttgggtgatg aaggaacttt agtaagtatg
    14941 caattagaag aattaatggg tacaaccaga atagaccaga aattaatatt caaagattat
    15001 aataaagaaa acacagaaat aaaagaactt atgaaaaagg tcaaaaattt aaattcagaa
    15061 gaactaatag aattggtcaa tatggcaaaa ctattagggt atagtggttt ttcagaaagt
    15121 atggatatgc ctataaaaac aagaggttat aggattctta gcaaaataca tagactacca
    15181 acagcaataa tagaaaactt agtaaattat tttgaaaact ttcaacaaat tttagatgca
    15241 tctattgaag aattagatga ggttgaagga ataggtgaaa taagagcaac atatataaaa
    15301 aatggactca taaaaatgaa acaattagtc ttattagata gacacatatg atgaatttta
    15361 aatgaatttt actacttttt tcttgaatat aatatacact ttggtctata atagataagt
    15421 ataactaggg ggtgaagcat tgataaggaa agtaacaaga atattgttta ttttacttgg
    15481 atttacaata ggtataacaa cctatttaac attaatgaaa gattttgaaa tattaacatt
    15541 tggaaaagaa acatatggtt atatcgcagc tgtagtagca ggtataataa tagccatttt
    15601 aggatatctt atagaaccat gggttgtaaa taaagtaaaa gaaattgcta aaatagtgga
    15661 taaagaattg tcaaaatatc ctcagactga tatattgtta gggtctatgg gtcttatagt
    15721 aggatttgtt attgcgtatt tattaagtgg attagtaaat agaataccaa tagtaggtgg
    15781 aatactatcc ttactattat atttattcct aggatatctt ggaatgaaag ttgcgcttaa
    15841 aagtaaaaat gatttattta atattggaaa actaggaaga ttagcaaatc ctattaagga
    15901 taaagataag gataaagaaa ataaaaaaga agtaaaagcc attcctccaa aagtattaga
    15961 tacaagtgta ataattgatg gaagaatagc tgatatttgt aagactgggt ttatagaagg
    16021 aaaacttatt attcctgctt ttgttttgga ggaactaaga catattgcag attcttcaga
    16081 tgatttaaaa agagtaagag gtagaagagg tctggatata ttaaatataa ttcagaaaga
    16141 attaaatata gaagttgaaa taagtgagag agactttgat gatatagcag aagttgatag
    16201 taaattatta aagcttgctc aggttttaaa tggaaaagtt gttacaaatg attataactt
    16261 aaataaagtt gctcaattcc aaggtgttga agtattaaat ataaatgagt tagctaatgc
    16321 tataaaacca gttgctatac caggtgaaga tatggttgtt caagtggtta aagaaggaaa
    16381 agaggctcaa caaggtgttg catatctaga tgatggaact atgatagttg ttgatggagg
    16441 aagaaaatat atgaatgaga ctataaaagt actcgttact tctgttcttc aaactcctgc
    16501 aggtagaatg atatttgcaa aacctaaaaa ttaatatgtt caaagaaacc tataatttgg
    16561 gctgtttatt ttaaatagat ggtccaaatt tatttattta taacgataat gtttaatgta
    16621 ttcttaaaga tggggattta aatcagaaag cagggagaat ttatgagaaa aaagattata
    16681 tctttcttag caacatttat cataatatta acatcagcaa gtcagtatag ttttgcagat
    16741 gatatgtcaa cacgtggaaa agtaatattt atagatatga atagaactag tatgagtaat
    16801 atgttaagaa taaaatcatt aagagaagaa ctagacaata gagggtatat tggcttgatg
    16861 aatataagag gcgataaagg aagtgatgat agaaggtcat atgctagtat gggtgctggt
    16921 ggtagagcta atgtggctaa tgaagaagat attaattttg aaagctcgag taaggataga
    16981 aatatagttt ttgaatcagc tacaggaaaa agtgcaaaag gtataaataa tttaactata
    17041 aataagtcta taaatgaaaa tttaaacttt ggagagtatg gttctgtatt agggtcatta
    17101 ggacaaagtt tatctgagaa tggattaaaa gcatctgtat tgggtaattc agatataatt
    17161 gaaaatggtc aacttataaa aaatagaaac ttatgtctaa ctgcaatgga tgaatatgga
    17221 agaataccta atggaaatgt agatactata aataaaaaag acttatctat gccttatgga
    17281 atatcaactg attatgataa attgattgta gagaccaaag aagcttacaa aaataatgat
    17341 gtggtttttg tggagcttgg agacacatat agattagatt tatataaacc aaatttaaat
    17401 gaaaaaacat atgagagtgt gaaagataac atagaaaaaa atatagatgt atatttaaaa
    17461 aatatatttt ctatggttga agaaaatgac actgtctaca tagcaagtgc atttccaagt
    17521 gatttagatt ataaaaataa aagaagactt tctccaataa taaaattaaa tggagaaggg
    17581 aaaggtatat tatcatcttc aactactaga agagaaggta ttgtaacaaa tttagatgtt
    17641 ggggttgaaa tattagacaa ctttaatata aaaaatcaaa atatggttgg aagaaaatat
    17701 gaacttatta acagagatga taataaagaa tttttgatgg atgaatatca aaagatagta
    17761 tctatttcaa gcataaggtc tacaatactg aatggttttg taagtatagt tttcttgtca
    17821 tggatagttg ctatgattgc aattttattt agaaagcata tatcaaaaaa ttataaagaa
    17881 acaacatttt ttatattaaa ggaactttta aaaataggaa tagtaatgcc actgtcattt
    17941 atgataacac caataatgaa tttcaaaaca ccattagcaa tatctttagg aataattata
    18001 ataacactta ctatatatct tataagtaaa gttttaataa agaatgattt aaaaaacatg
    18061 ctgtttttta ctggattaac catagtaatt atggttattg atgctgggtt tggctcatat
    18121 ttaatgaaaa gcaatgttat gagttatgac tgtataattg gagctagata ttatggagtg
    18181 ggaaatgagt atcaaggtgt ggcaatcggt agtgcaatat ttacatttgc aattttactt
    18241 acatataaaa atataccaaa gtggtctata atagtttttt ctttggttat cttaattaca
    18301 agtgcatctc caattatggg agcaaatgtt ggaagtgcta tttctgaatg tgttgcattt
    18361 ttactattta tactattgat atataatgtg aagatagact tcaaaaaaat tgtattatta
    18421 ggtatagcag tattatttgt attaggagtt tttgtagcaa tagatatgat tttgggaagt
    18481 aattcacatt taggtatgtt tgtaaaagaa atttacttta atggaccagg agaaataata
    18541 caaacattta gtagaaagat agagatgaat ttaaagctag ctcaaaccag tgcatgggta
    18601 aatatactgt taactggaat aggtgtaatt ttagttctta tgattaatca gattagatac
    18661 tttaagcaac tgatggatga gtatccaatt gtatttaaag gatttatagc atcaatagca
    18721 ggatgtttag taactctttt agtgaatgac tctgggattg tgtcatcagc tacagccttt
    18781 atttatgtaa ttgtacctat gataacatta agtataaatc tcacagcatt aaaagaataa
    18841 gtactgtatg aataatatgt aaatatgaac acattaattt aacttcttta tttacaaaat
    18901 aaagaagaaa gtttataaaa aataaattat aataaaaaag agtttacctc aaaattgatt
    18961 attctaatca aattttaagg taaacttttt ttattataaa gataaataac atataacctc
    19021 atattttagg tagatttttt tcttatatgt ttaggagtgc atttaaattt ttccttaaaa
    19081 attctgtaaa ataattttgt attagtaaat ccatgagctt ctaatatact gcataaggta
    19141 tcatttgtgt taattaagtc tttataaata tgagaaatac gcacatcatt taaatactct
    19201 aagtaagttt gtcccatata ctgtttaaat tttctacaaa aatattctgg ctgtaagtgt
    19261 gctaccttag cgattttgtt aatagaaata ggcattttgt aattaatctt tgtatattct
    19321 aaaactggtt caaattttga caaatcaagt actgtttttt tcatattttt attacctaca
    19381 gatattttaa agtttgtata taattcaaat aaaagttcaa ataataagct tgtaaagagc
    19441 agattagctg caggaggaga gctaagttta atttctctca tgttttctaa tataagttta
    19501 actttacctg aatttttttt atagttattg tcattggcat taaccttaaa gtcaaaatag
    19561 caatttttaa aatcagggat atatttgttt agaaaagaca atggtacttg caataatata
    19621 gaggtatctt gataagtaca tctagttgag tgaataattc cagaattaat gaacatacac
    19681 tcattagctt ttaaattata agtgtaaccc tccatgttaa tttgtaaatc accagaagtt
    19741 atataaatta tttctaatga gttatgccaa tgatttgcta cataactacc actatcatca
    19801 aatagtttga aattaacatc taaattatca ataggttcaa ttatttcatg tgaaaaatta
    19861 tttctgttca taataatatc ctccttataa ttttaaatta taaatagtaa atttataatg
    19921 ttcaatatta aaaagataat atagactaat aaattagata ataaacatac ttaagaaaac
    19981 ggataaaatc aaatataata actgtatatt aaaataactt aaaaaaatta ttttattata
    20041 agtgcaaaat atacctattt aaataagata agaggggagg attttatgtg tatataaaag
    20101 gtgtaaattt aggaggttgg ttagttttag aaaaatggat gacctcaagt ttatttgaag
    20161 gcacagaagc tgaagatgaa tactatttac caagacagct ttcaagagag gcatatgaat
    20221 ctagaattaa gactcatcgt agtgaataca tcacagagag agattttgca acgattaaat
    20281 ctatgggatt taattcagtt cgtataccag taccatactt tatttttgga gattgtgaac
    20341 cattcatagg atgtgttaaa gaacttgata aagcatttgc ttgggcagat aagtatgggc
    20401 ttagtatatt gatagactta catacagttc caggaagcca aaatggattt gataatggag
    20461 gaatatcagg tatatgtagc tggtcacaaa atccagaata tgtagcgttt acattaaatg
    20521 tattagaaag acttgcaaaa cgttatggga tgagacatga attgtatgga atccaaattt
    20581 taaatgagcc tataacagaa agaatgtgga acataatgaa tgttccaaat cggtttaagg
    20641 ctgtagataa ggagatggca agggggagta aacctaattc attagagttt ttacgtgatt
    20701 tttatataaa agcatataag gtaatgcgtc cttatatgag agaggaaaac gttatagtgt
    20761 ttcatgatgc atttgaatta aaagcatgga aagactttat gagagaggaa gaatttaaaa
    20821 atattgttct agatacacac caatatttga tgttagctga atcagatgga tgtgaacaaa
    20881 gtattgatag ctatctcaaa tatattagag aaaattatgc taaagatatt ttacaaatgc
    20941 aaaaatattt tcctgttata tgtggggagt ggagcttatt taattcatat gcttgtggta
    21001 tagatacaaa tggaggtcaa tcacctctaa atgggattga atcaaatata gataaattat
    21061 caaaagacga taaaagagaa ttatatagga agatagcaaa agcacagctt gatgcatgga
    21121 gaaatggaag tggtcactac tattggaatt ataagttatt actagataca gttaatgaag
    21181 aaggttggat aggatgggat agttgggacc taggcaaatg tgtagctcaa gaatggtatc
    21241 ctatagaata ttaaactaaa attggaggaa aagatatgat taaaaaccct atacttccag
    21301 gttttaatcc agatccttgt atttgtagaa agggagatga ttattatctt gtagtttcat
    21361 cttttgaatg gtttccaggt atacctgttt atcactctaa agacttaaaa aattgggagt
    21421 tatatactca tattttgaca gatgaaacaa aaatagattt gaagaagctt ccttcatcta
    21481 aaggaatttg ggcaccttgt ttgacttatt gtgaagaaga agatttattt tacatagttt
    21541 atggaattat gaactctatg aatgcaagat attttgatgt tgataattat ttaattacat
    21601 caaaagatat aaagggagag tggagtgaac cagtatatat acactcatca ggttttgatg
    21661 cgtctattct tcatgatgat gatggtaaaa agtggattgc atcattagac tgggagacta
    21721 gagaaggata tgaaaaacct ggtgttatat gtttagtgga atactgtaca aaaaagaaag
    21781 aaatagtagg atatccaaag agaatatggt cagggggaac tgatagaggt tgtattgaag
    21841 caccacatat aacaaagcgt ggagattact attatataat gtgtgcagaa ggtggaactg
    21901 gatatggtca tagtgtaact atgggaaggg ctaaaaatat atggagacct tatgaaaaag
    21961 attcaatgaa tccaattgta acttcaattc caggcgattt ttatgaaaga catgaccctg
    22021 accatctaaa gcctaaatac tataatccag aatcaaaact tcaaaaatct ggtcatggaa
    22081 gctatataga aacaacatca ggtgaagtat atttagttca tctaacatct agaccatttg
    22141 taccagagtt aaggtgtact ttaggtagag aaacagctat tcaaaaaatg aaatggacaa
    22201 aggataattg gcttcgaatg gaagatgaaa gtaatttggc aaaagaatac gtatcagaaa
    22261 gtaaactaga agaacattta gtttcatcta taccaagttt tgatgacttt gattcaaatg
    22321 tattaggttt acagtactat gctcctagga tttcaccatt atcatttgca gatgttaagt
    22381 ctagaccagg atatgtaagg attagaggtc aagaatcaag aacatcatta aataaagtaa
    22441 gcatattggc aagaaaatta acttcagtat atgcaagaat aaccactaag atggagtttt
    22501 acccagaggt acatcagcat agtgcgggtt tgataatgta ttatgataat atgaattata
    22561 taaatttaag aaagtattat agtgaaacat tgggtcaaag tgcactatca ataatacatc
    22621 ttgaaaatgg agagaagacg gaatttttaa atactaggat accaataaaa gatataccaa
    22681 tatatcttcg tctgtatata caaggtcgta agtcatattt tgaatggagt tatgatgaaa
    22741 aaaactacca aagaatagga aaggtatttg atacaactaa gttttcagat gagtactgta
    22801 aatatggaga atttacaggt acgtttatag gattaacttg tgcagatcgt gtaaagcata
    22861 aacactatgc tgattttgat ttttttgaat atatagcaga tgaatcaaaa gatgtcgatt
    22921 aataaaatca ttactaaaat tttcagagca aaggtaaatc ttatgctctg aaataagaaa
    22981 cattagaagg caaatcttac aaaaaggagt atggtattat gaatacagaa gttaatgtta
    23041 aagcaactac gattagatat gaaaagattt cattttttga aagaatttct tatggttgtg
    23101 gggatttggg gtgcaatatc atatactcag ctatgtcagc atttttgctg ttttattata
    23161 ctaattatgc agatgttagt gcagcagctg taggtagtat tatgcttgta tctaggatac
    23221 tggatgggtt tagtgactta acaatgggaa ttatagtgga tagaactaaa tccaaatacg
    23281 gtaaggctag gccttggatt ttgagaatgg cgattccttt tgctatagca gcagtattat
    23341 tattttctgt tccatcaaat cttggtatca cttctaaatt aatttatatt tttattacat
    23401 ataacttagt ttctactgtt atttatacag caatcaatgt accctatgca actctaaatt
    23461 cacttataac tcaaaaccag tatgaaagag gagtattaag tatatttaga atgatattag
    23521 ccacttgtgg taccttacta ataaatggac ttacactacc tctagttgaa tactttggaa
    23581 ataatttatc agcatggact aaaacattct ttgtatttgg aatagcatct ataatggtat
    23641 tttttataac atttactgga accaaggaaa gagtaaaagc agttaagcaa gataggaatg
    23701 aagtaatacc atttaaaata ggtattaaat ctttatttag aaataagtac tggatacaaa
    23761 taacattatg tttagtttgc atatttattg tatttgcgat aaatggtggt tcatccgtat
    23821 attatgcaaa gtttattttg ggagatgaaa aattatttgg tcctataaat atggtttcaa
    23881 atatatccca aattatagct atgtttatgg ttgctccttt tataaaaaaa tttggaaaaa
    23941 gaaatgtatt aatagtaggt tcaataatat taatatcgtc aaatgttatg tttataatag
    24001 ttggacaaaa ctatatagga gtaatatttg caagtgctat aaagggaata ggaagtgctg
    24061 gaatagcatc aacaatgttt gctattgttt cagatactat tgaatatgga gaatggaaga
    24121 ctggatatag aactgagggg cttataaata gtgcatctag ttttgggttt aaagtgggaa
    24181 atggattagg ttcagcaata ttaggtgcag tattatcaat tggtggttat gtaggaacat
    24241 cagctactca aagtgattta gctatagtat caataaaagt atgttttata tacctaccta
    24301 tatttataac aatattacaa attatcataa tgttttttta taagcttgat aaagaatata
    24361 gtactatact taatgaatta aatactaaat aatgaaaaag tttcttataa agtcttaagt
    24421 ttaattaagg tctattgtaa atgttcccta taaaaactat tatatattta tgattattga
    24481 agaattttta gagaagcgaa taataaaaat ataagaagac tttaaatgat gaaatggttg
    24541 gtcttttaga aatgatttaa taagttattt ttatttaaag cattataagt ggacaattaa
    24601 tttttaattt atggagatga tatttaacaa ttagaagtaa tatataatat gcgctaagaa
    24661 gcaatttttc aactatttat gaaggtggtg gtacaagaaa agtatcacct tttttttgtt
    24721 atacaaaaaa acagttgact ttttatatga accccttcta taatgttact gaaattaagt
    24781 aaaatgattg aacaaatgta aagataattg aaaagcttga ttttggagga aatcaaatga
    24841 aaaaaattgg agacttaaga ttaatgatga agtgttgttc actttattac gaagataatt
    24901 tgaatcaaca ggaaatagca aatcaattgg gaatatcaag accaacaata tcaagaatat
    24961 taaaagaagc ttttgaacaa ggaatagtaa aaatacagat agttgatgtt ttgaaaaatg
    25021 attaccaaaa aatagagaga agtttggaga gaaaatataa attaaaagaa gtaatagtag
    25081 tagatgataa acaagatgca ctaacacaaa aacaagaact agcaagagct gtatctgaat
    25141 atttaactag agtagtaaag gaaaatgata taataggtgt ttcaatagga acaacactaa
    25201 aagaaatacc aagatatgtg gaaaaaagta actgtaaaaa tgttacattt atacctctat
    25261 taggagggat tggagacaat gaaatagata ttcatgcaaa tcaaatagca gtaagtctag
    25321 cgagggcttt tggaggtgat tttaaattat tacatgctcc agctgttatg tctgacttgt
    25381 ctacaaaaga gaaactttgc aaagacgaaa aaattaaaga agtactagat ttaatagata
    25441 aaacaactat agcaattgtg ggtataggaa atcctatgag tttaaactca acaataatgg
    25501 cttctggata catgaatgaa gcagatatag aagatttaaa aaaatacaac tcaataggtg
    25561 caatttgttt acaagccttt gataaagaag gaaaaacttc tatacttgaa tttaatcaaa
    25621 gagtattagg agtgaaactg gaagatttaa aaaagataaa aagaacaata ggtgtcgctt
    25681 caggagatga aaaaatagag gcaataaagg catcattaaa agcgaaattt ataaatagtt
    25741 tagcaattaa tcacagttta gcacttaaat tattagaaga ttgtgactaa tagaaagata
    25801 gataaaagtt aacaggaggg aatgtatatg tcaatatcaa aagaaacatt gcttgaaatg
    25861 tataagagaa tgaatcaggc tagaaaattt gaagagaaag ttagttggtt ttttgcaaga
    25921 ggtatggttc atggaacaac tcacttatct gtaggtcaag aagcgtcaag tgtggcggct
    25981 gtaatggctt tagaaaaagg agatttagta tcattaactc atagaggaca tagtcaattt
    26041 ataggaatgg gaatagacct aaataaaatg atggctgaac ttatgggtaa agaaactgga
    26101 ttttgtaagg gaaaaggagg ctcaatgcat atagcagata ttgagtcagg taacttagga
    26161 gcaaatggag ttgtaggtgg aggtcttaca atagctccag gagctgcact tacacaacag
    26221 tataaaaaaa caggcaaaat agttttatgt agttttggtg atggagcatc aaatgaaggt
    26281 acttttcatg aaggaataaa tttatcttca atatggaaat tgccaataat attttactgt
    26341 gagaataatt tatacggtat gtcaacaagc ataaaacgtc atatgaatat agaaagtatt
    26401 gccactagag cagcttctta tggaatagaa ggaatatcaa tagatggata taacccaata
    26461 gaagtatacg aaacagtaca aaaagctgct gagaagtgta gaagaggaga aggacctgta
    26521 ttaattgaaa gtagaactta tagatggtta ggtcattcaa aatcagatgc aaatgtatat
    26581 agaacaaaag aagaaataga atcatggaaa gcaaaagacc ctattgaatt tttaaaaaat
    26641 tatcttatag aaaataatct atctaatgaa gatgaattag ataagattca agaatttgca
    26701 aagcaatcaa tagaagatgc agtagagttt gctcaaaata gcccaaatcc aaaaatagag
    26761 tctttattag aggatgtata tgcagactaa gaatggtaaa ggaggaaaat aaaaatgagt
    26821 acaagggaat taacatatgc acaagcaata aaggaagcaa tgtcagaaga aatgagaaga
    26881 gatgaaaatg taatatttat gggagaagat ataggaatat atggaggggc atttggtgta
    26941 tcagttggta tgatagatga atttggacca gaaagagtaa gggatactcc tatatcagaa
    27001 gcagctatag caggagcggc agcaggtgca gcagcaactg gtttaagacc tattatggaa
    27061 gtaatgttta tggactttgt aacaatttct atggatgcaa ttgtaaatca agcggcaaaa
    27121 atgagatata tgtttggagg aaaagcacaa gttccaatgg ttgttagatg tccaggtggt
    27181 tctggaactg gttcagcaga gcaacattca caaagtttag aagcttggtt ttgtcatgta
    27241 ccaggagtaa aagtagttgc accttctaca ccagcagatg ctaagggatt gttgaaagca
    27301 gctataagag ataataatcc agtcatattt gttgagaata aattgttata cagaaaaaaa
    27361 ggttttgtgc cagaagatga ttatgtaatt gaaattggaa aggcagatat taaaagagag
    27421 ggtactgatg ttacagtaat tacttatgga agaatgttac aaagtgttga agaagcagca
    27481 gagactctat caaaagaaaa tataaatgtt gaaattatag atttaagaac tttatatcca
    27541 ttagataaag agactatagt aaagagtgta tgtaaaactg ggagagtttt aatttgccat
    27601 gaagcagcta agactggtgg gctaggtgga gaaatatcag ccctgataac agaaagtgaa
    27661 tcatttgatt atttagatgc tccagtaaaa agaatttgtg gaaaagatgt gccaatacca
    27721 tataatccag agttagaaaa agcagttgtc ccaagagttg atgaaataga agaagcaata
    27781 aaatctttaa ttgttagata aggagggata gatatggtgg caaataaaat aaaagccaca
    27841 ccagcagcta gaagtcaggc aagaaaagac aatataaaat tagataggct gattggaagt
    27901 ggagaaaatg gaaggattca tttagtagat gtgttaaatt atttaaaaga taataaagca
    27961 aatactactc ctttagctag aagaatagca gaagatttaa atatagattt agaaactatt
    28021 gtaggtactg gatatagtgg aaagataaga aagtgtgatg tggaaaaatt aactgcaaaa
    28081 gaaactattg tatcaaataa tacatctaaa tatagtgaga aaaaagagct gaaaattgaa
    28141 aatgaaaatt caagcatgtt taacacagta gaaggaatat ttgaaaagcc aaatccaatg
    28201 agagctactg tagcaaaacg aatgtcagaa agttatttta gtgctcctgt atttacattc
    28261 aatatagaag tagatgctac agaattgaag gtacttagag caaagttaat cgacacagta
    28321 aaagaatcaa ctggagtaaa attaactatg acagatttaa tagtaatggc agtatctaaa
    28381 atacttccaa atcatcaagc cttaaattca gcatggacag atgagggtat atttagatat
    28441 aaagatgtaa atatagctat tgcagttggt ttagatgaag gcttatatgt tccggtagta
    28501 aaaaatgcaa acaaaaagtc tttaaaagaa atagcaaaag aaagcaaaga acttgcggaa
    28561 aaagtaaaga cagggaaact tatgccagca gaccaagaag gtaatacatt tacaataagt
    28621 aatgttggaa tgtatggtat aactacattt acacctataa taaatatgcc ttcaagtgca
    28681 atcttagggg taggtgctac acaagataag tttgttccag taaatggaga ggctaaaata
    28741 aaacctataa tgaacttatc attaacatca gaccatagag taatagatgg aacagtagct
    28801 gcaaaattct taaaagattt aaaagaattg ttagaaaatc ctttatcaat gcttgtatag
    28861 gttttgatat ttcgttactg atataaatag tttgaataga ttatattatt ataattgttt
    28921 cagtaaattt aaagctttta tatattaaga attattttta acagtaacta tatgtgaata
    28981 tgatttgctt aattaaaata tatgaaaata aataggagga gagcctatgt cagtagaagt
    29041 aataatgcca aaagcaggag tagccatgga agaaggtact atagtatctt ggctaaagca
    29101 agaaggcgaa gaagtaaaga ttggagaacc tatactagaa ataacaacag ataaagttaa
    29161 tatggagata gagtcagaag gagaaggtac acttgctgta atcatacata aagaggaagg
    29221 tgaagtatta ccagtattta cagtaatagg tgtaatagca gaaaaagggg aaaatcaaga
    29281 ggaagtaaaa gctaaatatt tatctggaaa tgtttcaaaa gaagatatag ttgaagagaa
    29341 tcaaaatata gaagttaaag aagaaaaaat aaacaaaaaa gagtgtaatc atgattatga
    29401 tgtagtggta ataggaggag gacctggggg atatttatca gcattaaaag cagctctttt
    29461 aggtggaaga gttgctttag ttgaagaaaa tatactagga ggtacttgct taaatagagg
    29521 ttgtatccct acaaagactt atataaaaac agcagaaata ttagaagaaa ttgaccaatt
    29581 atcaaagaga ggtgtaaaag taactgtaga taaagaacag gacattaaga aggccataaa
    29641 atataaaaat agagtagtta aaaaacttac agctggtgtt ggcggattat taaaaagtag
    29701 agatgtagat gtgtttaatc ttaaagctag tgttaaagaa gagcataaag taattttatc
    29761 agatggaaaa gttttagata cagaaaatat aataattgct acaggttcca aagtaaggat
    29821 attacctata aaaggaatag aatcaaattt aataataact agtacagaag cattagattt
    29881 ggaaacagtg ccagaagaat tagtaataat tggtggtgga gtgattggtt gtgaatttgc
    29941 agagatattc aattcaagag gttctaaagt aactatagta gaaatggaag atagagtaat
    30001 cccaagaatg gataaagagt taagcgaatc tttaaaatat tctcttagta aaaaaggtat
    30061 aaatgtatta actaaaaaga aagtttctga atttaaagaa gaaggaaata acatattagt
    30121 gtgcattgaa ggtgaagtgc caataaaagc agatttatgt ctatatgcta taggaagaga
    30181 agctaatctt tctggcatag aggacttaga tattaaaata gataaaggtt caatagttgt
    30241 aaatagtaaa atggaaacaa gtataccaag catatatgca gtaggtgatg ttactggagg
    30301 tgtaatgtta gctcatgctg cttttaaaat gggagaggta gcagcttcaa atgctctagg
    30361 tgtgaataaa gaagttgact taggtgcatt acctagttgt gtttatacaa ttccagaagt
    30421 agcatcagtt ggtattacag aagaggatgc tagaaaaaaa tataatgtaa aagttggtaa
    30481 gtttaatttt gctggaaatg gaagagcttt agcttctggt caagaacaag gatatgtgaa
    30541 agtagtagca gatgctaaat atggagaaat tttaggtata catatgtttg gatgtggtgt
    30601 agcagaactt ataaatcatg cagcatcatt taaagcatta gagataccta ctgatgaagc
    30661 aagtgagttg atatttggtc atccatgtac ttcagaagct ttaatggaag ctttggcaga
    30721 tgtaaatgga gaatgtttac acttacctaa aaaataaatc taaacataaa ttattaattt
    30781 aagtctatta ttaatattaa tttatatcat aaaattttaa aacacttact tagccatacc
    30841 ccttattatg caaataattt atttgtataa taaggggtat tttttatgga aaaatataga
    30901 atataga
//
LOCUS       DAELXX010000037        30314 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.037, whole genome shotgun sequence.
ACCESSION   DAELXX010000037 DAELXX010000000
VERSION     DAELXX010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30314
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.037"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..756
                     /locus_tag="KPH29_001640"
     CDS             <1..756
                     /locus_tag="KPH29_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HBG1467243.1"
                     /translation="KKTQNSVKEILKENKINEKDIDTFIKAVNNYNKLQVKILQNNIN
                     ISKSGYSSINAKQVPYNLEKLQDNWVKKFPDYMDVNCRITAFRLFKDFINSNKKFTGD
                     SIDLNVDLDTIMNNKDAKFSTKDVEKFINFFSAIPAKDTDDTIKIAEQIKNEWKKRKI
                     SFKNNKNISIINGFLRYPETKNVFIGHTGICIKTKNGILFLEKYGVTSPYQVTKFKNK
                     KGVKNYMFNRLKMSEGEIELPDPIIMENDKLMK"
     gene            complement(1069..1848)
                     /gene="hisJ"
                     /locus_tag="KPH29_001641"
     CDS             complement(1069..1848)
                     /gene="hisJ"
                     /locus_tag="KPH29_001641"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HBG1467244.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKENL
                     NKYGKELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNTILEDLIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLELVKKYDIPLVLGSDSHCAEEINKYDII"
     gene            complement(2388..2738)
                     /locus_tag="KPH29_001642"
     CDS             complement(2388..2738)
                     /locus_tag="KPH29_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HBG1467245.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(2754..3170)
                     /locus_tag="KPH29_001643"
     CDS             complement(2754..3170)
                     /locus_tag="KPH29_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HBG1467246.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(3189..3380)
                     /locus_tag="KPH29_001644"
     CDS             complement(3189..3380)
                     /locus_tag="KPH29_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HBG1467247.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(3531..4346)
                     /locus_tag="KPH29_001645"
     CDS             complement(3531..4346)
                     /locus_tag="KPH29_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBG1467248.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            complement(4393..5331)
                     /locus_tag="KPH29_001646"
     CDS             complement(4393..5331)
                     /locus_tag="KPH29_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG1467249.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSVGVDKIGIAIDASNKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYIYLKENDVTISLFAFTPVRGTKMEKIN
                     QPSIESYRRVQLMSYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(5328..6200)
                     /locus_tag="KPH29_001647"
     CDS             complement(5328..6200)
                     /locus_tag="KPH29_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG1467250.1"
                     /translation="MNKNLSEKMKIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEK
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(6225..8153)
                     /locus_tag="KPH29_001648"
     CDS             complement(6225..8153)
                     /locus_tag="KPH29_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4445 domain-containing protein"
                     /protein_id="HBG1467251.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNSL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKETLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(8265..8642)
                     /gene="gcvH"
                     /locus_tag="KPH29_001649"
     CDS             complement(8265..8642)
                     /gene="gcvH"
                     /locus_tag="KPH29_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBG1467252.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(8729..10855)
                     /gene="cdhC"
                     /locus_tag="KPH29_001650"
     CDS             complement(8729..10855)
                     /gene="cdhC"
                     /locus_tag="KPH29_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HBG1467253.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(10912..11718)
                     /locus_tag="KPH29_001651"
     CDS             complement(10912..11718)
                     /locus_tag="KPH29_001651"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBG1467254.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(11768..13135)
                     /locus_tag="KPH29_001652"
     CDS             complement(11768..13135)
                     /locus_tag="KPH29_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HBG1467255.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(13166..14110)
                     /locus_tag="KPH29_001653"
     CDS             complement(13166..14110)
                     /locus_tag="KPH29_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HBG1467256.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(14130..14900)
                     /locus_tag="KPH29_001654"
     CDS             complement(14130..14900)
                     /locus_tag="KPH29_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG1467257.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(14956..16341)
                     /gene="lpdA"
                     /locus_tag="KPH29_001655"
     CDS             complement(14956..16341)
                     /gene="lpdA"
                     /locus_tag="KPH29_001655"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBG1467258.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVIEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTVKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(16385..17266)
                     /locus_tag="KPH29_001656"
     CDS             complement(16385..17266)
                     /locus_tag="KPH29_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBG1467259.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMQKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(17302..17976)
                     /locus_tag="KPH29_001657"
     CDS             complement(17302..17976)
                     /locus_tag="KPH29_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HBG1467260.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(18013..18885)
                     /locus_tag="KPH29_001658"
     CDS             complement(18013..18885)
                     /locus_tag="KPH29_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HBG1467261.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLVKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(18977..19609)
                     /locus_tag="KPH29_001659"
     CDS             complement(18977..19609)
                     /locus_tag="KPH29_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HBG1467262.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKIEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(19714..21390)
                     /locus_tag="KPH29_001660"
     CDS             complement(19714..21390)
                     /locus_tag="KPH29_001660"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBG1467263.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(21639..22418)
                     /locus_tag="KPH29_001661"
     CDS             complement(21639..22418)
                     /locus_tag="KPH29_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG1467264.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(22728..24647)
                     /gene="cooS"
                     /locus_tag="KPH29_001662"
     CDS             complement(22728..24647)
                     /gene="cooS"
                     /locus_tag="KPH29_001662"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBG1467265.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKAGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            complement(25254..26165)
                     /locus_tag="KPH29_001663"
     CDS             complement(25254..26165)
                     /locus_tag="KPH29_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBG1467266.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(26281..26403)
                     /locus_tag="KPH29_001664"
                     /pseudo
     CDS             complement(26281..26403)
                     /locus_tag="KPH29_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
     gene            complement(26486..27628)
                     /locus_tag="KPH29_001665"
     CDS             complement(26486..27628)
                     /locus_tag="KPH29_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG1467267.1"
                     /translation="MDFKIEKYLLKKAEELAFITIKHGGEFKLKSYKVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELGINK"
     gene            complement(27646..29400)
                     /locus_tag="KPH29_001666"
     CDS             complement(27646..29400)
                     /locus_tag="KPH29_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HBG1467268.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMKINDVNGLVYRQGNEIIVNKPMELLQDLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKQSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            29536..29742
                     /locus_tag="KPH29_001667"
     CDS             29536..29742
                     /locus_tag="KPH29_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467269.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(29747..>30314)
                     /locus_tag="KPH29_001668"
     CDS             complement(29747..>30314)
                     /locus_tag="KPH29_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467270.1"
                     /translation="HNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMARIKA
                     IQLIDKYIDLKSEDYDEESILLNVLNVLYDVYMEDRTVENEGINSIKKSILSILGEDS
                     KLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIIDPIFNQITV
                     MSKTFNDNILSIKINSKSGIYILKFKKV"
BASE COUNT         9153 a         6069 c         3366 g        11726 t
ORIGIN      
        1 aaaaaaactc aaaatagtgt aaaggaaatc ctaaaagaaa ataaaattaa tgaaaaagac
       61 atagatacat ttattaaagc agtcaacaat tataataaat tgcaagttaa aatacttcaa
      121 aataatataa atatatctaa atctggatat tcttcaatca atgcaaaaca agttccatat
      181 aacctagaaa aattgcaaga taattgggtg aaaaaattcc ctgattatat ggatgtaaat
      241 tgtagaataa ctgcttttag actttttaaa gattttataa attcaaataa gaaatttact
      301 ggagactcta ttgatttgaa tgtcgactta gacactataa tgaataataa agatgctaaa
      361 tttagcacca aagatgtaga aaaatttatc aacttctttt ctgcaatacc agctaaagat
      421 acagatgata caatcaaaat tgcagaacaa ataaaaaatg aatggaaaaa aagaaaaata
      481 tcttttaaaa ataataaaaa tatatctatt ataaatggat ttcttcgtta tccagaaact
      541 aaaaacgtat ttataggtca tacaggcata tgtattaaaa ctaaaaatgg cattctattt
      601 ttagaaaaat atggtgttac atcaccttat caagttacta aatttaaaaa taaaaaaggt
      661 gtaaaaaatt atatgtttaa tagattgaag atgtcagaag gtgaaattga attgcctgac
      721 cctataataa tggaaaacga taaacttatg aaataataac tttaaacgca aaatagtatt
      781 tataattact aagtcaatat aatagagaag gtctatagat ttgtgtaaat acaatcttat
      841 ctacaatctt atataagaat gtttatataa ataagataga ttaattctaa gtcctattat
      901 tctattgaat tttattatta ttttacttag attctattgt tattttactt agaacttatt
      961 gttattttat ttaagttcta ttactatttt caatcatata aatttacaaa agactgtaca
     1021 taaatttatt ttatacaaac ttatctacag tcttttgtgt gaatatatct aaataatatc
     1081 gtatttattt atttcctctg cacagtgaga atcagaacct aaaactaatg gaatatcgta
     1141 tttttttacc aactctaata agtatccatc tatataaggc tctttgcaat actcttttct
     1201 atttccagaa acattataat caagttcata acctttttct ttaattaact ttattaagtc
     1261 ttcaagaatt gtatttcctt tataatcgta tggaaatacc tgattaaact ttctaactaa
     1321 atttaaatga cctattcttt ttggtttatg tatacctaaa tctgaattta cagcttttat
     1381 caatgtttca taatacttat tataaacagc ttcaatactt cctaatttgt cgattaaatt
     1441 ttgaaactcc tctacactgt aatctacaca ataataatca tcatcaattt taattatatg
     1501 tactgataaa agagagtctt ctaattcctt tccatattta tttaaattct cctttatccc
     1561 ttcttcgtat ccttctataa aatccacttc cgacccaaca tttatcttga ttttaccctc
     1621 atacttttct ttcaacttct tgacatcatt aaaatacttt ggtaaatctt caatactcat
     1681 acaactatca ttttcaggag cagggtctct aaatccattt gcatacggaa aatgttctgt
     1741 aaatgtcatt tctgttattc caacatccat tgctctttgt acatattttt caaaatcatc
     1801 cttacttcca tgaggacaat aaggtgaatg tatatgacca tctttcataa ataattcact
     1861 ccttttttaa agaaaaaatc taatataaat gcataaattt tttaacttca aaataatatt
     1921 tttaatcatg ctaattttta ttatatctaa tattagagta attttaaact gccttttaag
     1981 tatcttatct ttagtactat tttcaactta acattaatat tattttttaa cttaacatta
     2041 atactatctt taaatagtaa tactcaatat ataattaatc aaataactca aattgtttga
     2101 tgaagctttt gcagtattta aagaatatat aattatttaa cattaaataa tccaatcttg
     2161 taataaaatt tactcattaa agtatattac agaaaatatc tcaaaataaa ctttttagtt
     2221 catgatatac tcttttttgc atgaaattag atatattttt atcatttcaa caatgaaagg
     2281 gaggcttaca tccatcttga gataacccct tagataaaat ttttaataat taaacatcat
     2341 aaaatataaa aatcgcttat atcaaacgta taagcgatta attatattta catatgtttt
     2401 ttagcctcta acaacatttt attagcttct tccatatact ctatagcctt ttcaatactc
     2461 tttgctgttt cttcttttcc aatagctcta gccttttcaa cccactctct aaatccttct
     2521 tcatgagttt cattgtgatt tatccaatgt actaaaagta tttttaaagt tttttcatcc
     2581 ttagactctg atgattcctc atgactatgt tcatggtcgt ggtcgtgagc atggtcatgt
     2641 ggatgttcat gacatatatc tccatgagta tgttcgtggc agtggtcatg gtcatgatgt
     2701 ccatgatgat atcttacaat ctctttatca aaaatcatta gttattcccc ctgttatttt
     2761 atattaaact ctttttttat aaggtttaca gaatctttta caagtagtgc taatggttta
     2821 ccttctaatc caactaactc cacattttct ggcataatag gtaaaagtat cttaagtgcc
     2881 tcactgtctg caatagcttc acttatatta caagtaactt ctcccatcat agaattaggt
     2941 attattacag aaataggtgc aactattata tttgcttttt tagctgatac tactatagca
     3001 ttttcccctg tagctccttt attagcatga gctttcatca tagaactagt tgctatagaa
     3061 ttagtcccta gtgcataaat ttctacatat gtaggtaact cttctctcaa agaagaaact
     3121 atctgggctc caatacctcc acccattcca tctatcactg ctattatcat atgaaattcc
     3181 cctattaatt aattttcttg tattattatc ttatggtcca tcaatcttat ttctttaatt
     3241 atcccatcta ttatcttttg ctctcctaat aaatccgtta aataaatctt tccatcacaa
     3301 gggtctatat taaccacatt ttccataacc ttttctaact cattattact tttacaaatg
     3361 aaagctgatg attcacacat ataaatctcc tcctaatatg atttttatta aaaatcaaaa
     3421 aaagattccc ttaggtattt caatactcca aagaaacctt catgatttca gtttttgtat
     3481 attattatac cttaagtata tcactttaat ttatgtaaag tcaattaaag ttaacgcttt
     3541 ttattatact cttcattctt gtatttttca tatagttctt taactaattg cttctcataa
     3601 tcagtcaaat cgccctccac aaactcaaca ttaaattgtt cagctaatcc ctttactatg
     3661 tttttctgta gaatatctat gtctattttt ctgccaattt cattttctat tccacttgct
     3721 tttttagctg taaatttcat agctctttct ttcaattctg gtgtcttagt ttttattaat
     3781 ttaaacaatt tttctacatc aaaatccagt attattgaac catgttgtaa taaaacacca
     3841 tcttttctgc tttgagcact tccaattacc ttcttattat taatagtaac ttcatatgat
     3901 gcatgtgcat taaaacatgc agctgataaa ttttccctac ttattctctc gcctctattt
     3961 aaattatctg tttctatacc acttagattc aatcctttaa ctagaccttc acttatatat
     4021 ctataagata ggttgatgct cttatcgatt aaaggattat cttctcctat aattatacta
     4081 taagttagct cattatcatg taatacagct cttccacctg taattcttct agtatagtct
     4141 atattcatac atctacactt atctaaatct atctcatctt ccaatttctg aaaatatcct
     4201 atactcaaac aagctggttc ccatgtataa aatctaagag ttggcttatt atgtcctttc
     4261 ttatatgcag taaatatagc ttcgtcaatt gccatattca ttgctccttc atatgattta
     4321 ttatgtataa ctctccactg attcatgatt attacgcccc catttttagt ataaatatat
     4381 cctagaaatc ctctatagat ttatttctct tattgctaaa tctatctcac tttgatttaa
     4441 aggtcttgaa tagttgtata tagtacttcc gggtctttca ttatagtatg gtctattaca
     4501 atccttacaa cctcttattt caaaaggata gcctttgttt atgtctttta aaatatcatt
     4561 gtcaagcttt atactattta agtatccatt tttaaactta aaatactctt ttggataacc
     4621 tttatttatc atataagaca ttaactgtac ccttctataa ctttctatgc ttggttgatt
     4681 tatcttttcc attttagttc ctctaactgg agtaaatgca aataaactaa tcgttacatc
     4741 attttccttt aaataaatat ataaattgta tatatcttca tgactttcac ccataccaac
     4801 tattatatgt gtacttattt tattgggata tgattttgac atttcagtta taaattttaa
     4861 cttttcatca tagttatttc cttttatttt ttcatatagt tctttatttg atgcatctat
     4921 tgctattcct attttatcaa ctcctactga aaaaaacttc tttatctgtt catcattttc
     4981 taattttgca gatagtgaaa ttggcatgtc tatcttacca cttatatagt ttataaagtc
     5041 taaaacagag ttatgtgctt cttcacttgc cattgattga atacatattc tctttatatt
     5101 ttttccttta taagctttta aagcatctaa tatttcttct tttgaaaact ctggccaaat
     5161 tactcttgat aatttatctt ttctagtact actttctatt gattgagaac aaaaactgca
     5221 tttatttata catttttctc caatcattat atatgctgtg gtaggaggta tatcgctttt
     5281 tttgtttaat atcccaagct ctattgctgt cccaactgat agtctaatca taaaacgcaa
     5341 cactccttac actcatttat tgtcatattc atttctatag ccttattctt tgcagacctt
     5401 gatggtaata ctattctatt gactccacac attatagata attggtctag ctcttttcta
     5461 tacactcctc ttggtctcat acaacctaga ttaatttcag tatctggtaa attaattcta
     5521 gcttcacata aaatatctgc taccccttct aactcaggag gttctacatt ttcatactca
     5581 gtcccttttg ttggaataag gactataaat gtaagcttat taggaggatt tttctgtagg
     5641 tattctataa tttcatattc accctttatt tgtcctcctt ttaaaccaat acatatatgt
     5701 ggtgctactt ctgtatgttc ttgtattgta ttataaactt caatataatc ttctttactc
     5761 tttttcatct tataaacttc tcttattgtc tcatcatcaa acactaaatc aaaagaaact
     5821 atatctaaat acttacacaa ctctacaatg ctatctttat ccataagacc taaatgagca
     5881 ttcaatctat atcctttctc ttttaagttt ttaatggtat taatatgagt atttataggg
     5941 acatctccct cataactaca accgccactc aatagaaagc tagttatatt ccttgattgt
     6001 actttttcct catattcttc tattggcacc atattgttta agtagtgacc gttgcaatgg
     6061 gcacatttca aactacattg attgcttgta gtactaagag ctagtgtctg gttaggataa
     6121 gcaaactcta ttttatttcc aaaattcttt aatttaatat caaatgctat tttcattttt
     6181 tcagatagat tcttattcat agtttattcc ttcttaagta cattttactc ttctagtaat
     6241 ttctttgctg ttgggaactg ctctatattc gtatgtggta agaaacatgc agaaataaat
     6301 tcatccatat aacttggata tacactaagt tcaacataag ttatttgatt acctatttct
     6361 tcaagtttat tttttgcatc tttacttatg agagctaagt aagaaccaac aagtgaacta
     6421 ttccctatat aagtaaattt ttctctatca atgtcaggaa gtaaccctat aattattgag
     6481 ttttctatgt ctaggttgtt tcctatacca cctgctatat aaaccctatc taatatacta
     6541 aaatccatac ctaaactatc tacaagagtg tatgcacctg aatatatagc tcctttagct
     6601 ctgatgaaat tatctatatc aacttcattg actactatat cattttctaa atcaaattct
     6661 tctttaaatg ctaatacata ctctcctatc tcatgctcat taaatctaac tctcttatta
     6721 tctaaatccc tgtatatttt acctcttcta tctattacac ctttagtaat catttgacag
     6781 attaaatcaa ttataccaga accacatata ccaactggtg cacattcatc aataatcttt
     6841 aatgttggct ctaaagtttc tttatcaata attactttct ctattgctcc tgctgatgcc
     6901 ctcattccac agcttatccc tccaccttca aaagcaggtc ctgctgaaca tgcacagctc
     6961 atcatataat ctttatttcc aaatacaatc tctccattag ttcctaaatc tataaataaa
     7021 acattttctt cacttgacca tatccctgct gataatacac ctgctgttat atctcctcct
     7081 acataacttg caacagatgg agctaagtac acatatgcac tgtcatttac aaaaagacca
     7141 acattttcac ccataagttt tggagatttt aaaaatggtg gtatgtaagg ttcttgtctt
     7201 aagaagtctg tatacacacc caagaataaa cttgtcatag ttgtattccc ggcagccact
     7261 aatgtaacta catcttcctt atctattcca tttctttcat atattgtttt taatagtgga
     7321 tttatagttt cctcaactat agctttatga agtgtctcta atccattttt cttagttgaa
     7381 tatataattc tatttataac atctgctcca tattttatct gtgcatttcc agaagatgct
     7441 ttatctacta cctcttttga atataggtct actaaacaaa caactactga agttgtacca
     7501 atatctattg caactccgta taaggaattt tctttattac cttgttctat atttatgatt
     7561 gtaagtttct tttgtttttg aacatatgtt attgtaacct taaaatcact ctttctaaat
     7621 acagttggca tttttctaag tatatccaat ctaaaatcta tttcattata tcctaggcta
     7681 tttctaacat atctttccaa tctatctaca tcacttatat tatcatctaa atttggttct
     7741 tccatttcta tgtatttctt ttttatattt gttttaaatt gaaggttatg ttcttcaatt
     7801 atttttttag ctctttcaaa aatttcccta tcctcttttt tattactacc ttctatcttc
     7861 ataccatgca tagaagatga tactttagaa ggaacttcaa tttctatatc tgatattacc
     7921 tttgtacaac aagctagtat ataaccttgc tcccactcat catctgttat atgtcttgtt
     7981 ttttctgtat caacttttcc attcaataat tttactttac acttcccaca agacacgttt
     8041 ccattacaag gagcatctat gaatatatct gcatttcttg caacctctag taatatatca
     8101 ccctcattac aataaacctc tttattgttt ggtgtaaaac tcacttttat catgttatcc
     8161 ctcccaattt gttctatata tgataaaagg aaagtatagc taaatttgcg tgcactcaaa
     8221 ttttactata ctttccccaa ttataataat atcttaatct ttaattattc taatccagcc
     8281 tcgtattctt tatcacttaa taacgtatca agttcagcca catcagctag ttttactttt
     8341 aatatccagt tttcatatgg gtcttcattt atacattctg gctcatcttc taatttttca
     8401 ttaacttcca atacctcacc attaacagga gatattaaat cagaagctac tttagaagac
     8461 tcaactactc caaaatctac tcccttagtt acagtatctt ctacttctgg tgtttcaaca
     8521 aacaatattt ctcctaattg atcttgagca tagtctgtta tacctatata tacaacatct
     8581 ccatcaatta cttttaccca ctcatgatct ttagagtatt ttaattctgg taataatttc
     8641 atttcgatag acccccaata ttatattatt gattaaatta cttaaatcat attaatatta
     8701 tatcatatta ttttaaattt tccatcaatt acattacact ttccatagct aatgctggat
     8761 gacctttttc ttctaagaaa gctaatactc cttcagaatc gtcagctata gtttcatcac
     8821 aaaccatatc acagaagttt tctattccag tcatttcttt aactgtagca tttaatttat
     8881 cagctacata atcttttaat tcttttggca tccaaactat tctttctggt ccaccttctg
     8941 cataagcgaa ttttttagaa gatatgaaat gtcttccgtg tcccataaat cctggagttt
     9001 gaactcctcc acctgtcata gaagcaagtt ctccgaaagt cataccaact ggagtaacac
     9061 ttgcaaattc tctgttaact acaacaaatc cgtttgcttc tggcattata ccacagatac
     9121 attcgaagca tccacaagaa gtcattgggt cttctagtat agaatataat gtaacagatt
     9181 caactgcacc ttgagatatt tggtttacag tttcatttac tgaattccat acacctgttc
     9241 tatcatctaa gcactcgcct ttttctattg gttgacaagg acctgttggg tctaattctt
     9301 tagtagcctt agcatctaac cagctaactg ctccacaaag accaagtctt tcaggagtaa
     9361 caacacaaac gtgagctggt gcaaatgatt gacataagtt acaagaatag aaagtatcaa
     9421 cactttcatc aactaatgaa gctaatcttt catctctagc attgtactta gctattgctt
     9481 ctccttttaa ttcagacact ttttcagcat ctgtaattat agttatttca cacttatcaa
     9541 ctactgattc aaattcatct aacatcttag catataaaac ttctcctata tgctcaagtc
     9601 taaatccttt atcaaatgca tctttagaga ttcttaccca agccatatct ctttgtccaa
     9661 cgtgcattac accttctata tagttcaaga agtagtggaa acgtctttca agaactggct
     9721 cgaaatcttc ttgcatattt ttaccagcta ttttaactat tacagcaagc ggtaatctta
     9781 atacaccatc tgcatcaact tcatctatat ttggtcctat tatttctatc ttatggtctt
     9841 caacttctgt tgcttctttc ttaacaacaa gctcccaagc ttcagttctg tttccaccaa
     9901 actcagcaaa catatcgctc tttctaattc tctcaccttc aaatgctgct gcaaatgaaa
     9961 ctgggatagg tatttcagta acttttattt ttattcctct agcttctaat gaagtagcta
    10021 ctatcttatc gtaatctttt tgagttaata ggttcattgg aacttcaaga actgtttggt
    10081 cagttataac tgggaatcct aaagctattg ctccagcacc tgcagataca actaattcac
    10141 ttaatggtcc aaatgcatta acaaatgcag gaactctatt agctgtgtac tctaataatc
    10201 cgtttaaatc accaggagtt aatccaccga atattaatgc tgctcttact gcaacagata
    10261 caacgtgtat aacagaagtt acatcatatc ctagaggtat aactcttagc tcaagaccca
    10321 tttttactcc agcttctata gcttggtcta taactttacc aactaagaaa gttagtaaac
    10381 ctttagattg gtaatctttt ataacttttg ctgctgattc tgcatctgga cactcaccaa
    10441 gaacaactgc aacaccaggt atatctcctg taactaatgg tacaccaagt gatctgatta
    10501 tagggtcagt tatatgacct gcacatggct cactatatgg agcatccata gttgaatatt
    10561 ttaatgcttc aattacttct gcagctaaag ccgtagctaa accagcatta aaagcatgtt
    10621 ctagtaaatg agttctattt attaaagatt ttactacttc taaagcacct tctaagtccc
    10681 ctaaagtgtt catcttttgt cctgttgcag catatataca aggtaatgaa tatgctgtat
    10741 cagggaaagc aactttatgc tcttttccat tcttttctat agcttcagct aacatagctt
    10801 gagccgcacc taaagcttgt tctgaccctg taaagattat attatataga ttcattctat
    10861 ttcccccttt aatatttaca cctatttttt attaactata ccctaaaaac cctagaaagc
    10921 aaatccacct tcatttaact ctaagaaaga gtctgcatat aaagatgccc cgttgattat
    10981 gtcacaagat tttacagttt tcattaattc tgggtcacat ggattcatta tagctgaact
    11041 aaatccatta gccattgcca ttgctaagaa tgcactatct aaaacaggtc taacatgctt
    11101 aggacatcca ttagatacgt tagataatcc tcctgtagtt aaaagtccca tctcagtcat
    11161 catttttata gcttctaaaa cttctacttg tttttcttgc atacctttta taactaagca
    11221 taatgggtca aatagtatat cagttggctc cattcctaac attaagcctt tttctaatat
    11281 ttcttggcag taagccatac gctcatcatt atctcttggg ataccttctt ttgcacataa
    11341 acctataacc attgcttcgt attcagctgc tatatctatt aaatcaaatc ttgagccagc
    11401 atcagcagag tttattattg gttttgcatt agttctatca taaactttaa gtccagcttc
    11461 gatagctttt ttattagctg tatctaatgc tataggaaca ttgttgaact cagattgaag
    11521 taatttgatt ccccatgtca ttatttcttc gccatctctt tcggcaggtc ctatattaaa
    11581 atctatatag tgtgctcctg cttctaattg ctcttttgct ctttttaaga ttggagctgg
    11641 gtctctttct gctaatgctt ttcttattga aggtgatata caatgtattc tctcacctat
    11701 aatcataaat ttttccattt ataatgtccc cctttaaatt taatattaat agttagttaa
    11761 tattttatta gtttgtttgg catgcttttt cttggtatcc ttttaagaac ttaggtaatt
    11821 ccatagattc ctctggtcct ataactacat tccatcctgg taagttatct tctatatcac
    11881 ctttaagtac tgcaactttt cctggaatga taaggtcttt acaattagtt acttcttcta
    11941 ccttactttc tttaatgaaa gcagagattg agtttccacc aaatttacca gcagcccaag
    12001 atgtaagaac tgaatatcca cctgcatctg gtattactaa ccatactgga acttttgatc
    12061 tttctatatc accagcaact atgaagtaag ttaatgcaaa gtcaacagta actaatactg
    12121 gagaattttc atctgggttg ttgattggat aaatctttgg ttcaactctc attggtcttt
    12181 gtgggtcagt gtatatgttt tgtcttagtg caaataatgg taatgcctta gcataactaa
    12241 tatcatctat tactattata gaaccgtatt ttatagtgaa tattgatgat aaagcaactt
    12301 ccatcatagg gtttgaatta gataatctat ttgcaaatac tattgaagga tatccaaatg
    12361 ttctatcttg ctcttttaaa gctgttcttc taacttgtat agcattagtg taagtatctt
    12421 ttatgttttc tcctgttaca tctaatacta attctttata tccagcggct tgaattaatt
    12481 caactgtttc atataattct tctaagctac cagcttttac acctaatggt aaacttgctc
    12541 ctttaactac ttctatcata tctttgtagt tctctttagt tgctccatat accattggtc
    12601 tagcatcttt taatacttca acagcttctt taactacttg tgcatcatca caagctaaaa
    12661 tataagctac ttctaatcca ctgcctttta ttttatctat taattttaaa tacgcatctt
    12721 tatctccaca atactctaaa acagccattt caactttcat ttgttcacca attcttacat
    12781 agtcaacttt tttcatgtta gctattttag catctactgc ttcatcactc atacaagtac
    12841 agaatgaaac tgcatatcta tttctactta ctaatgtttt ttcatgtcta aataatactg
    12901 tttctccacc tagttcatac tcagatgcac cagcaccaac ttttaaagct ttcattaaag
    12961 gtgcagtagc ctctgataat tttgctatag cgtcatcaga catatgtggg catttaccta
    13021 cttctacagc tcctgaagca accttcatag agaaagccat acaagttggg aatccacaat
    13081 ccttacagtt tttctttggt gttaatttaa atatatctaa agcttttaat gccattattt
    13141 tttcccccta accttaacaa tttaattaag ctaatgcgtt aaccaattct tttatagttt
    13201 ctattgattt tggatgacga agtataactg cgtttgaacc acctactaaa acacttgctg
    13261 cagttgaaac ttccattgct atagttcttt cttctggaca accccaatct ggctcatctt
    13321 ctattggagc tatagcttct ttaacatgac ctacttcaaa agctactggt gttatgatag
    13381 gcatttgtaa tgtcttatca ttttgaccaa atgctgcaag tctaattcta tccatagtag
    13441 atgcaacgta ctcatatcca taaccaactg ctgaacatcc aacattcata actatatttt
    13501 cacctttaac gcctagttga gttaacaata cgtttaattg tttagctagg tttatatcaa
    13561 cagaagattc agctcctact ttatgtgcat aagccatacc agctgatgct cctactcctt
    13621 tgtagttatc ttctactgct gacataaata agcagttatg tccatccaat gtttgagcta
    13681 atttttcaaa taatttacca tctttttcat gatttcctga accagctaca actaaaggta
    13741 attttattgc ttcaattact gccttagcta catcagcaca ttcatcaact gacttatcta
    13801 atccatttgg gtcagaacca tcaaatttta aacaaataaa atctgcttgt gtattagctt
    13861 caacatattt agcccactct actggacaat tagctacatc tttgtataat tccttatatg
    13921 aatcagtcca gctttcagga taaacgtctg atatttgtat acctattttt ggagaattac
    13981 ctacttctcc atcaaaacta taaaaaggta atacattttc cccacctaat ttaattgctt
    14041 tttcccctat tcctacttca acttctgata tttttccaga atatttttga gtagacattt
    14101 taaatgccat atttttttcc cccttaagcc tattttaatt ctaatttagc aaaaatttct
    14161 ttcatagcca ctttagattt tgaatcttca ggtaaattaa ctaaaggaat acctgtagaa
    14221 tcatattcat atatcaattc atccataggt acaacaccga ttagctctag gttatgcttt
    14281 tcaatttctt ctttaacacc atcattcata accccatttg gaactttatt tacaatcaaa
    14341 agtattcttc caacacttaa ctttagttcc tcagctaaat ctcttattct agcaacagct
    14401 tgtatgctac gtcttgagca atcactaacc aataaaagtg tatcaacatg tctagtgact
    14461 tttctactta gatgttccat accagcttcg ttgtccatga ctaagtattt gtaatgacca
    14521 gatattttgt ttacttgttc tctaagtatc ccatttacaa aacagtaaca tccttcacct
    14581 tctgacctac ccattactaa taagtcatat ccttcgcctt cttcgattat agaatttaat
    14641 ctaaattgaa gatattgtgc ctttgtcata ccaccaggga atgcattgcc taacttttct
    14701 ctttgattta cttcttctct tatttctcct attgttgctt caacttcgat acctaacact
    14761 tcatttatgt tagcattggc atcagcatct acaactaata ctggtccctt tttgtcctta
    14821 accaaataat caattaaaag ccctgtgaga cttgttttac cagttccacc ttttcctgca
    14881 acagctatat tgtatcccat tttaagactt cccccttaaa tctagccaca gagccttatg
    14941 tactctgtct gtctattata atttaggtac agaatgaaca cattctccat gtacatcatg
    15001 aagtgcttcc attagccctt cagataaact tggatgtgga tgtattgcat cccctacttg
    15061 ttctactgtt aatcctagat gaacagcaag tgacaactct gtcaataaat ctgtagcatg
    15121 aggtcctact actgctgcac ctattatctt atcagtttct ttgtcagcta taacttttac
    15181 aaatccttgg aaatgtccta tagcttgtgc ttttccaaga cctctaaaat caaattgacc
    15241 tacattgtat tctacacctt cagcttcaag ttgcttttca gtcttaccaa caccagcaac
    15301 ttcaggttca gtataaacac atcttggaat tgctctataa tctactacct tagtcttacc
    15361 taaagcattt tcaactgcta cgataccttc tttagaagca acatgtgcta ggaaaggagt
    15421 atctattata tcgcctattg cgtatatacc ttctacatta gtttcaagat gttcattaac
    15481 tactactttt cctctttcca tttcaattcc tatatcttct actccagaat tgtcaaggtt
    15541 aggtcttcta ccaacacata ctaatgcata ttgtgcttct ataacctttc cattagaaag
    15601 agttgcaacc gccttaccat caactacttc acaagtttgt actccaatac ctgtaataac
    15661 cttaatttta tctttcttaa attgtctaag tagttgttta gctacatctt tgtcttcatt
    15721 taatagtatt tggtcaacca tctcaactat agttacttct gttcctaagg ctctaaagaa
    15781 ctgccctatc tcacatccta taactccacc acctactatt aacatagact caggtatctc
    15841 ttcaagtcca agaacttcat cactagttat tacaactttt ccatcatatg ggaacattct
    15901 tggtacaact ggaacagaac catttgctag tattatctta tctgctttta tagtttctac
    15961 tgttccatca tctttagtta cttctatagt gtttttatca attaattttc caaaaccatt
    16021 aactaagttt actcctcttt tttcaaataa gaactctata ccacttatta attggtttac
    16081 aactttattt tttctttcca ttatggcagt gaagtttggc ttaactgtac catctatttc
    16141 tataccaaaa tcctttgctt cttttacagt attcaatact cctgaagaag caagaagtgc
    16201 tttagttggt atacatcctg catttaagca agttcctcca actcttcttt tttcaataac
    16261 cgttacatct gcaccaagca tagaagcttt tatagctgct acatatcctc ctggtccacc
    16321 acctactact actatcttca tccttaattc ccccaaacct aatataatct acaatcttgc
    16381 aatactataa acctgcggca tccagtatag caggtatatt ctcttctgcc ccaattgcca
    16441 ttatatgaac accatcacat aaatcttctt cttttaattg tttgataagt tcccctgcca
    16501 tttttattcc ttcacttacc cagttttctt ttccagctgc tcttaatctt tcgatttgtt
    16561 catctggaac gaatattcct ggtacatttg cagtcataaa tttagccatt cctggtgatt
    16621 ttaaaggtac tattcctgct aataccttgc aatccatatc ccttgttaat tctctaaact
    16681 ttctcattgt atttatgtca taaagtgctt gtgtttggaa aaatttagcc cctgctttta
    16741 tcttcttttg catttttaat aattgaactt ctattggaga atattctgga gttactgatg
    16801 ctcccagata aaaatctgga gaacctttta acttattacc agccatgtcc gtacctgcca
    16861 ttaaagtctc agcagtttga agtatcccca cagaatctaa atcaaacact cccttagcct
    16921 gtggatggtc tcctacgcta gtatggtctc ctgttagtgc caacaaattg tttatcccaa
    16981 atacacctgc tgacaacatt tctccttgta tagctattct atttctatct ctccctgtta
    17041 tttgaataac tggctctaat ccagcatctt ttaataattt acatgtagca agagaagttg
    17101 ctctcataac tgccgattga aagtctgtta catttgctgc atgaactctt ccaactaacg
    17161 gctttgcaca ttctattaaa tgtgaaaggt cagtcccttt tggaggagcc atttcagtag
    17221 taactgcaaa ttttccactt tctaatgttt ctcttaataa gctcatctct aatccccctt
    17281 ttaaccttct aaaaaaaact tttatgaatt tgctgctgct tctttctttt tctttgcact
    17341 taaacttctt ggattatttt gcttagaata gtcttttggt ggtcttattt ctgctaagtt
    17401 atctaattgt cctatagctt ctagtctttc atatatcttt atccaagcac aatcattctc
    17461 tgagtttact tcacacttac catttttagc tccaccacaa gccccattca taagaccttt
    17521 agcacacata gttactggac atatcccacc tgtccaacca agttcacagt ctccacaagc
    17581 cttacaggct tcttcatatt ctccaactct ttgtacttcc cctataaaca aagtattgtt
    17641 ggctggataa actggcttat cttttaaatt cttaactatt gtctgtgtac catctccaca
    17701 tgctaaagat aaaactgcat cagcttcctt agtctcttct tttagagcct ttaaatcctt
    17761 tttagacttt aaaaggttac aagcagggtc aagcattaca tatcctaaga tttttttgcc
    17821 ttcaccttca agagtttcct tcatttttaa aacttcttct tccccaccac ttttacaagt
    17881 tgctgcacat tgattacaac caactaaaac cagcttgtca aaatccttta agtaccctag
    17941 cacttcttct aatggtttat tttcagaaat tatcatatct acttcccccc taaaccttcc
    18001 acatatatat ctctatttat tgttttgtaa tttacaagcc ttaactacat gttttgcaag
    18061 tatagatgtt gtaactgaac caacccccgc tggaactgga gttatcattg atactttgtc
    18121 taaaactgca tttgtatcaa catctccaca taaatttcct tcttcatcaa catttatacc
    18181 aacgtctata actaccgctc catctttaac aaaactttca tcaaccatct tagctctacc
    18241 aatagctgct attagcacgt ctgcctctgc tgctacacct gatagatttt tagtttttga
    18301 atgacatatt gtaacagttg catgttcact taataaaagc attgatactg gctttccaac
    18361 taccattgat cttccaagaa ctgttacctt acttcctttt aaatcaacat tatagtgttt
    18421 taatatctca actactgctg taggtgtaca tggtggaaat ccacttttat ccccttccat
    18481 taccttagca gagtttattg gactaaaaca atctacgtct ttttccggtg caataacata
    18541 ttttataact tcttcattta agtgttttgg aagaggtcta aaacataata taccattaac
    18601 atttttgtca tcattaactc tctttaatac atcaatatat tcctcttgtg ttatatcctc
    18661 agctaactct aacacttctg ttgttatacc tatgttttga catctcttta gtgctcctct
    18721 ttcataagat aaatcatcac ttcttgcccc aactctaact attgttagtt taggatttat
    18781 accttcttta actagtaaat cgacttcttt tattaattct tcactaattt tatcagctac
    18841 aggttttcct tttataattt gtcctttagt tgacattcct tccatacatt cacaactccc
    18901 ctacaagcaa acttaaatta atttattcta tataccacat attaataaca tagtatctac
    18961 tttcgtcatt taaaaattat tttccaagag ctttttcaac tttggcataa acttcatcac
    19021 aaatttttac accatcttca actaacttat ctacttctat ttttacctta ttaacatatt
    19081 ctttatcttg tatagaattt atatttataa ctacatttaa ttgtccacca agtatagcag
    19141 ctcttaaaca ctgaactcct acaccaacat cacttatagc aagtcttgaa cctttatcaa
    19201 ctaaatcttc atgtaactta atagcttcat agcaaactct tactatgttt aatggtactt
    19261 cacaagcaac ttttaatgcc ttttccattg tttctgcttt tattcttttt tcttcttctg
    19321 tttctttagg aagcccgtaa gcttttgata atggtaagaa acattctgca tcatcatcta
    19381 tcatttttaa taaatctttt tctaactttc ctgccttact taatatctct tttacgcttt
    19441 cttcatactc agcatacttc ttcttcccta ttgtaaggtt acataccata ctacctaaag
    19501 ccatacctat tgcacctact agtgcagcag ctcccccacc acctggtact gcttctttgg
    19561 aagatagtac ttctacaaaa tcaacacaag ttttatctgc tattttcatg ataatcctcc
    19621 ttgaaaggat gtaggaaaat atctcaaatt attcgagata ttttacgaac tattttaaga
    19681 tatctcctac tctttattta taaattaatt tccttagaat aatccagcta ttactccatt
    19741 ttcatcaaca tctatttttt ctgctgctgg aacttttggt aatcctggca tcttcattat
    19801 ttctccagta agtgcaacta taaatccagc tcctgctgaa attgttgctt gtcttacagt
    19861 tattctaaat cctgttggtc tacctaattt agtttggtca tcagttaaag aatattgagt
    19921 cttagccata catactggaa ctttagtaaa tccaagtttt tctaagttag ctatttcctt
    19981 atcagcttca ggagtataat ctacaccatc tgctccataa atcttagtag ctatagcatt
    20041 tatcttgtct tttatagata aatcatcttc atagcagaat ctaaagttat tttcttcttc
    20101 atccaataat cttaaaactt ctttagctac tgcaattcca ccttctccac cttttgccca
    20161 tacttcagaa agagcaacat taacacctaa ttccttacac ttgtcttcaa caagtttaag
    20221 ttcagcttca gtatcaagtg ggaatctatt gattgcaaca actgctggta atccatatac
    20281 ttgagttatg ttttcaacat gttttaataa gtttggaagt cctttttcaa gagcttctag
    20341 gttttcgtta tttaagtctg ctttagcaac tccaccattg tattttaatg ctcttacagt
    20401 agcaactatt ataacagcat ctggttttaa gtttgccatt ctacatttta tatctaagaa
    20461 tttttctgct ccaaggtcag caccgaaacc agcctcagtt attacatagt ctgcaaagtg
    20521 catagccatt cttgtagcta taacagagtt acatccatga gctatatttg cgaatggtcc
    20581 accgtgtaca aatgatggtg ttccttcaag agtttgaact aggtttggtt ttaaagcatc
    20641 ttttaataat gcagccatag caccattagc ttttaattgt ctagctgtta caggttcacc
    20701 ttcatagctg tatccaacta ttatgtttcc taatctttct tttaaatcag atatatcact
    20761 tgccaaacag aacgctgcca ttatttcaga agcaacagtt atatcgaatc catcttgtct
    20821 aactgctcca tcaccttttt tacccatacc atcaacgata tttctaagtt gtctatcgtt
    20881 catgtccaca catcttctcc aagttatttt cttagggtct attctaagtg catttccttg
    20941 gtgaatatgg ttatcaagca tagcagctaa aagattgtta gcagctccta tagcgtgaaa
    21001 atctcctgtg aagtgtaagt ttatatcttc cataggaaca acttgtgcat atcctccacc
    21061 agctgctcca cctttaacac caaatactgg tcctagagat ggttctctta aagcaactaa
    21121 tacatttttg tctagtttag caaatgcatc agcaacacct atagtagtag tagtttttcc
    21181 ttcacctgct ggagttggat ttatagctgt agttagtatt aacttagctt ttttaccagt
    21241 ctctcttttt agtagattgt agtcaacttt tgctttgtat ttaccataaa gttctacatc
    21301 atcttcacct agtcctaatt tttttgcaac ctctctaata tcttgaggct tagcttcttg
    21361 agctatttca atgtcagatt taaatcccat aactgaatct cctcccacaa cctaaataga
    21421 tttaaaatat aatttttgta aaaattgtat taatatgtaa aaattatatt ttataagata
    21481 attatacatg taaaattact taaaaactag ctactaagct attaaaatat tcaaacttaa
    21541 tagctagtta tttatctaat tacttactta ttacctatct attgcctatg tctaaccttt
    21601 aaccaactta aaatcccaaa tcaaaatttc tatctatttt atttatcctt aagtgacatt
    21661 aatttatctt ttattgcttt aatctgttct ttcgttgttt cactagaatc atatggagat
    21721 tgattagctc tatcagaatc aataacatct tggttatagt aaataaatcc taaagattct
    21781 ccatcttcta agttttggat tataaattct tcatcttcct tatttctaat tttatttcct
    21841 actacaaata ctttatttac acctatatca tgtccaagtt tcttaacttt tctataagtc
    21901 tgtagacttc tctctcctgg ttctacaact actataaaag catctacacc ttgggctgtt
    21961 cctcttccta agtgttcaat tcccgcttcc atatccataa ccactacatc tttattttgt
    22021 agtattaaat gtgagcagag tctcttcaaa agaacatgct ctggacatac acatccagta
    22081 ccaccagaat ctacggttcc caatgtaagt aatcttactc cattgtattc tttacagaaa
    22141 ttttcaggga tatcgtctac tttaggattc atcttaaaca tttttccaaa tgaacctaca
    22201 gaagcagctg ttctatctga aactaatttt ttcatttctg atataggtac tattgattca
    22261 tatacttctt ttggaaaacc tagtgctaaa gctaaattag catcagggtc ggcatcaaca
    22321 gcaacaactc tgtatccgtc ttcagcaaac atcctagaga gcattgaaga aaaagtcgtt
    22381 ttacctactc cacctttacc tgttattgct atcttcataa tccctaccac cttttattac
    22441 tatttgcttt ttagtagtta attttacagt taaatatttt ttatactcta tatttttata
    22501 cactaaaaat aaactacagc tagtccttac aggaaccaat taaatttacc ctactagctt
    22561 ttcaaagcag catgtatagt tttattattc tttaatattg tttatatttt atttattcag
    22621 tatattcatt atatttcgat gttctttact ttaatatttg actttattca actttactca
    22681 accagaaact tcttataaaa acgtttattc tataacttca ctatttttta agcttcagcc
    22741 ttttctgcca ttttttcttc aactaatgct tcaaagtgaa cacgtttttt ctctatatct
    22801 tctattattg tagctgctaa ctcttttgga tttgtattta cagtaaatgt agctccaact
    22861 ttatctttta actcactagt taatatatcc actgctcttg aagaaccagc tattggaggc
    22921 attattccta aataagtgtc tataccagaa gctacaacat agcatcctat agcaactgct
    22981 ttttctgaca tccattctgg agctatacct acaactggaa ggtcacacat atccatatct
    23041 aagtaattag ctgctgctcc aactaaatct agtatacgac ttatatcaac acaagaaccc
    23101 atatgtagta ctggtgggat tcctactaat ttacagacag ttgcaagacc tttacctgca
    23161 tatttttcag ccgcatcaga ttccattaaa ccaaacttag ctgctgctga agctccacaa
    23221 cctgtagtaa ctactattat gtcgtttttg attaattctt tcattattgt tacgtgagcc
    23281 tcatttgaaa ctcctttagc attgttacat ccaactacac cagctgctcc tcttaatact
    23341 ccagctttta gacagtctgt taatggctta actgttcctg ctgggtctat atgagagttt
    23401 acaactctgt ctaattgatt tactactgct tctacactat acccaactgt tgcgcctgat
    23461 tttaattcag gtataagtac ttttgattta tctctatttt taaagtttaa aatagcttct
    23521 ttaactattg ctttagcatc atcaagtgct tgttcttctc taaattcaat ataagttgaa
    23581 cctgttatct tagcttttgg agaagttgtt acaaatttag tatggtaaca atctgcaact
    23641 ctagctaatg cagggaatat acattgaacg tcaactatta cagcttcaac tgctccagtt
    23701 attattgcaa gttcttgttg atggaagtct cctgctattt ttacaccatg tctcattgta
    23761 atctcattac ctgtacaaca catacctgct aagtttattc catcagcacc aacttcttta
    23821 gctaaagcta ctaagtctgg ctcttctgat gctaaaacta tcatttctga taatgctggc
    23881 tcatgtccat gtaatattat gttaacttta ttttcttcta atactgctaa attagcctct
    23941 gttcttcttg gcactggagt tccaaataag atatcgctta gacgagtacc tatcatagaa
    24001 ccaccccaac catcagccat agaagttcta agactcatgt gtattaaact atctatatca
    24061 ccagtacatc ctatatgagt agagtgcatt attgttgcaa tttctctatc tatagctctt
    24121 ggctctatag cgtattcttt ccatattttt tgtcttggtt ctggtgcatt ttttataaat
    24181 cttaatgtcc caaaaggttt tccaaattcc attaatgcaa tttctgccac ttcatgtgct
    24241 acatcataga tatctctacc ttctgtctct acatcccatt ctttagctaa agtaattaac
    24301 ttagcttcgt ctcttatttt atagtttcca tttggatcag ctaatgctaa tgtatgtgct
    24361 atatctctag catggtctga atgggcagct gtcccaccag caaccattct tgcaaagttt
    24421 ctacctacta tagtatgctc atctgctcca cataaccccc ttggagtctt agggcttatt
    24481 ctacatggtc ccattccaca tattctacag caaacacctt gaagaccaaa accacattgt
    24541 gccttaaagc cttcttttct atcatacatt gtttctacgc cttcttctct agctttagct
    24601 atcatcttct ggctgtttaa atcaattgtt aacatttttt catccatatt cctatcccct
    24661 ttttttgatt tttaaaatcc cccaggtgac tgacttcatg tcataaaata ataaaataaa
    24721 tgctttctaa gttgattata tactaatttt cagtgctttg ctttagattt aaatattttt
    24781 ttaacaaaat tagataatac ataaaaattt tattttctac acttaattta agttgatttt
    24841 atcgcttgtt tttggctatt tgttaatatc taaacctact tttttgatac ttcagtttaa
    24901 ttaaattatg ttttgatttt tttaaatcaa atttgaaatt tttctagtca tttttatatt
    24961 attgcagatt gtttgtgtat ttattatctt tcactactgt ttggacttat tacagatttt
    25021 tactccctaa acttaattag taaaaacttc ttagaatagc attttttgga ctaatttttt
    25081 tgaatttttt tatttttcct actttaatat taattatagc ttcaaattga acatttgtat
    25141 atattcattt gaagctataa ttatttttta tataaaactt ttttaataca gcattaattt
    25201 agacattttg actatttttt ctactttaat tcatttattt caagcgattt atcctatttc
    25261 atatttttag ctacaatagc cccatatgca atagaatata atttagattc atgtgaatca
    25321 gctcttgaca ctaaaactat aggagctttt gcacccataa ttatacctgc tgatttagag
    25381 tctgccatat atgtaaaggc tttccctatt ccattgccaa cctctattgt tggaaccaat
    25441 atgatatcta catctccaga tacttcactt ttaaaccctt ttatctcact agcttctttt
    25501 gaaacagcta aatcaaaagc cagaggacct tctacaatta cattttcacc aaattctcct
    25561 tctttacaag cttttgctaa catatctgca tctacagttg cttgcatctt aggatttact
    25621 ttctctttag ctgccaaaca tgcaactttt acagtttcca ttcctaaagc ttttgcagct
    25681 tttatagaat tttttaatat ctttgccttt tgttcataat caggagctat attcatacct
    25741 ccatctgtag ttataagtaa tttatggtat ttttctaact catataccat aacatgactt
    25801 aataaactat cagttctaag accatagtct ttatttaaca cttcttttaa tatgacagat
    25861 gtatctaata atcctttcat tacaaagtcg gcttctttac ttgatactaa tttaactgct
    25921 atttctgcac actctttgat atcatcctta tccactattt tgataccact taaatcaaat
    25981 cctatttctt ttgatatttc tcttattttg ccttcatttc caaccaatat aggagtaatt
    26041 atatttcttt ctacagcatc ctttattgcc attaaaactt ctttatcatg agctgctgct
    26101 acagagagta caattttctc tcctccctta agttgttcta aaatatcatc taattttttt
    26161 atcaaaagta acccctccct tatatatatt taactaaata ataccatatt tgctatacct
    26221 tttctcaatt tattttttat gttataaaga atttatatga ctaaaagaca gtttaatatt
    26281 ttattctggg atttcatcaa tagctcttaa aagctttgca ggaacaccac ctacaatagt
    26341 attttttgtt acaactgctc cagcagcgat aattgcatta tctccaatag taactcctgg
    26401 aactcttcat acaaaaaaga ggcttatctc tattttgaga tagccccact taaccgataa
    26461 aattaaatac aacctaacaa tatccttact tatttattcc aagttctctt agacaagcaa
    26521 tagtaatttc atcttgtgga tttaattcat aagctctttc tatatagtaa gttgcatcat
    26581 caatatcacc attatttaaa taagccattc ctaaattgca aagtatttct gggtcttcct
    26641 ttatcttagc cgcttgttca aaatactcta ttgctttatc taaattattc ttataagcct
    26701 cacaaagccc caattccact atagtatcca cctgattagg ctttattatc aatatcttct
    26761 caagatacat cttagcctgc tctatttcgc ccataccctt atatccaagt gctatcataa
    26821 acagcaagtt ccaccaatcc atatgttctt cttctaatgg aagtaacttc tctaatcctt
    26881 ccttaaactt accttggaat acaagtgtat atccttcttc atattgaact ttaaagtcca
    26941 tcttgcctag attctcttgt acctcagcta ctaaatcagc atctaaccct agccttaatg
    27001 cttcttccca agtcagctta gtctttaaat attgaccttg attataataa tggtagccta
    27061 agtgataata acctaatgca aaattctcat caacatcaat taccttttca agcttagcca
    27121 tggcttcaag taagaatgca ttcattgcct tatcatccat atctttttga tactgtttag
    27181 ctatctcttg gcaaacaatc gcatagttat acattgcatc taaatcatct ggatacattg
    27241 ttataaatgc ttttatatat attaaagaat ctgtatgttc accaatctca aagtattttc
    27301 tcgacatata tcccatatac gatttcaaat ccaaatcaat atcttttgat aaagcattta
    27361 aaaacttctc atactctgca ttgtttttaa acttactatc tattccaatt atatatatca
    27421 ttgcatctgc tatagacatt gaatttaact tatcttgagc agtcttttct tttatccctt
    27481 ttactaatac ctcatttttt ataggtacat ctaatccgcc ttttggcact ttatagcttt
    27541 ttaatttaaa ctctccacca tgttttatag ttataaaagc taattcttct gcttttttta
    27601 gcaaatattt ttctatctta aaatccattt tatcctccta aatacttaaa tctctacttt
    27661 atgagtttta gatttctcaa atattttttt atcatcatac tcaaataaga ctacattttc
    27721 tacttctctt atttctgtat ttatatcatt ttttaaatcc acaacattaa atctaaatgg
    27781 ctcaaaatga acatatttta ttatatgttt agctggttta tcaacaaaac taggtaaata
    27841 tgttgataag ttatcattat cttgtaaaaa tacatggcat ctattcttaa aatcatccac
    27901 atctagctta ttaaaaaact gtggtatatt tgatgtttta cccagtgata tatagtcata
    27961 ttttaatata tcattgaata catcattatc tatgttgatt ttttcattat agaaatccat
    28021 caatatttta tataactgat tctttccttg tgccaaatca aagtagccat tttcatcaaa
    28081 atacttggca aaaacctcaa agaatttaaa aggactttgt ttataaaatc tgcctataat
    28141 atatctcata gataacacaa agtttttaga attgtagtat ctctccagta tatcttctat
    28201 atcctttagc ttcaaagttt cttcatagct tatataatta ttgtataaaa actcatatgg
    28261 agcataatcc ttatatttaa aaccatgctc atctgcaact tttctcatac ctgtcccttt
    28321 aatcatcttt aaaaagccta attgcagatg ctctatacct agattaaata catcattaaa
    28381 agaattttca aagctcttat aatcttcata aggcagtcct gcaattaagt ctaagtgctg
    28441 atgtatattt ctataagatg ctacagtttg tacaacatga gacaactttt taaaatcatc
    28501 ccttctacct actgcatcaa gtgttttttc attcgttgac tgaaccccta tttcaaattg
    28561 aaaaagacct tctttacaat cagccaaaaa ctcaagcatc ttatcatcta tcaaatacgc
    28621 tgtaacttca aaatgataag ttgtatatcc attatcattt ttcataagga aatccatgat
    28681 ttccatagcc acttttctat ttgcattgaa tgtcctatct ataaacttta tctgtgatac
    28741 tcttgcatct attaaagctt ttaaatcact ctttatcctg tctaaactaa aataccttag
    28801 acctggaata gtagatgata aacagtactg gcagttaaaa ggacaacctc ttgatgattc
    28861 ataatacact attctatttt catattcttt tggattcaag ttttcataag gactaggaat
    28921 ctcatccaaa tcttgaagta attccatagg cttattcact attatctcat tgccttgtct
    28981 atatacaagt ccatttacat catttatttt catctttcct tgcaagtgaa gtactaagtc
    29041 tctaaaaatt aattctccct ctccataaag tatataatcc acaaaatcat actttttcat
    29101 tgcctcataa ctatcataac tcacttccgg tccgcccaaa gctattttag tattaggtct
    29161 aatttttttt agattatcac aaagtttaac tatatctccc acattccaga tatatgttga
    29221 gaataatatt atatcatatt catttttata tatatccttt aaaatatagt ccaaatcgtt
    29281 gtttatcgtg tattctttca tattaacttc tatcaaatct cttacaaatt cttttagata
    29341 tcttatagcc aaatttgtat gaataaactt ggaatttaat gttgttaata aaattttcaa
    29401 atatttctca cctcttttta agattatact gtctttttgt accccttata ggttaaaata
    29461 gtatatgtaa agtctaaata atatctatat aattaattta ttaatttcaa gtaatgtgag
    29521 gaaaggaagt tttttatgga attaattgaa aaagtatcta tcaactcttt atcaaaaaga
    29581 gatttactac ttattataaa gtctcttgaa tacactaatg aaaatactca aattagtgat
    29641 ttcattgacc ttaggaataa tatagtaaag gaattatgtt ttttaactga cactaaggaa
    29701 aaagattttt tagattatct agaaaaaaat agcacactct aataacctaa acctttttaa
    29761 actttaatat ataaatacct gatttagaat ttatttttat gcttaatata ttatcattaa
    29821 aggttttcga cataactgtt atttgattaa atatagggtc tattatagta ttaccttctt
    29881 caagtcttat aagacttctt gggtctatgc ttttattata tgccttaact ccaattttat
    29941 attttctcaa tttaacaaca tattcggaca tagaaaaaat aaaatctata ttatcttcat
    30001 ttaatttact atcttcaccc aaaatggata atatagattt ttttatgcta ttaattcctt
    30061 cattttctac agttctatct tccatatata catcatatag aacatttaaa acatttaata
    30121 aaatactttc ttcatcataa tcttcagatt tcaaatcaat gtacttatca attaattgta
    30181 ttgctttaat ccttgccatc tgaaacttta caacatctga tttgtctaat tctatagaaa
    30241 atcctattat cttgtcttta atactctgta gtaattcgac atactctatt ttactattat
    30301 ttataagatt gtgc
//
LOCUS       DAELXX010000038        30084 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.038, whole genome shotgun sequence.
ACCESSION   DAELXX010000038 DAELXX010000000
VERSION     DAELXX010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30084
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.038"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            46..120
                     /locus_tag="KPH29_001669"
     tRNA            46..120
                     /locus_tag="KPH29_001669"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:78..80,aa:Thr,seq:tgt)
     gene            complement(280..957)
                     /gene="srtB"
                     /locus_tag="KPH29_001670"
     CDS             complement(280..957)
                     /gene="srtB"
                     /locus_tag="KPH29_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HBG1467271.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTKTSSELQKK
                     EYKKEDLSKINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKNNNKIKITLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNNESDYQNYINDITSKSLYKSPIKVNSNDKIVTLSTCTYEFDDARM
                     VIHGRLI"
     gene            1089..1423
                     /gene="rnpB"
                     /locus_tag="KPH29_001671"
     ncRNA           1089..1423
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="KPH29_001671"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            1701..4244
                     /locus_tag="KPH29_001672"
     CDS             1701..4244
                     /locus_tag="KPH29_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HBG1467272.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQDKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESISQTIHNEYK
                     KLCGLEELHSVEGYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGAKEEVDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEVEQEEENLNDISPDIILDKPVENHQAKSEEIEQNEL
                     KEIKQEEPSQHIEEERSVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            4366..6357
                     /locus_tag="KPH29_001673"
     CDS             4366..6357
                     /locus_tag="KPH29_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467273.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNEDTGVKYIYSFSDNKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCDIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKVLLNLSKNMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLN
                     NSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            6347..6667
                     /locus_tag="KPH29_001674"
     CDS             6347..6667
                     /locus_tag="KPH29_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467274.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            6865..7734
                     /gene="galU"
                     /locus_tag="KPH29_001675"
     CDS             6865..7734
                     /gene="galU"
                     /locus_tag="KPH29_001675"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBG1467275.1"
                     /translation="MTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIEEA
                     IESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHYIR
                     QKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGVQK
                     IAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSILEK
                     QTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENLKD
                     DLMDYMRKKVSEK"
     gene            7759..8772
                     /gene="galE"
                     /locus_tag="KPH29_001676"
     CDS             7759..8772
                     /gene="galE"
                     /locus_tag="KPH29_001676"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBG1467276.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            8884..10818
                     /locus_tag="KPH29_001677"
     CDS             8884..10818
                     /locus_tag="KPH29_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HBG1467277.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKAINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLSV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSLEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            10973..11836
                     /locus_tag="KPH29_001678"
     CDS             10973..11836
                     /locus_tag="KPH29_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HBG1467278.1"
                     /translation="MLSSYVKRVSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEY
                     KYAVSQGDYVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDE
                     LPLFLSRLECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARI
                     VIESRDDVSNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIA
                     DILCNPLSRARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIV
                     EPVGPWLEKMR"
     gene            complement(12044..12925)
                     /locus_tag="KPH29_001679"
     CDS             complement(12044..12925)
                     /locus_tag="KPH29_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG1467279.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
     gene            13338..14186
                     /locus_tag="KPH29_001680"
     CDS             13338..14186
                     /locus_tag="KPH29_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBG1467280.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGTEEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            14265..16187
                     /locus_tag="KPH29_001681"
     CDS             14265..16187
                     /locus_tag="KPH29_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG1467281.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDIHDSSVIAAYLEGCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            16269..17132
                     /gene="aroE"
                     /locus_tag="KPH29_001682"
     CDS             16269..17132
                     /gene="aroE"
                     /locus_tag="KPH29_001682"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBG1467282.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGIEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNAKDDF
                     YKRAEQTVENIKKDVPECVVNLYDLEDTNKLYEEIESSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            17332..18549
                     /locus_tag="KPH29_001683"
     CDS             17332..18549
                     /locus_tag="KPH29_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG1467283.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAILIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLIVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            18785..20650
                     /locus_tag="KPH29_001684"
     CDS             18785..20650
                     /locus_tag="KPH29_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HBG1467284.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEDSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFLI
                     AINVLKDENIEYNKIEFEKEINDILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKIVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(20828..22192)
                     /locus_tag="KPH29_001685"
     CDS             complement(20828..22192)
                     /locus_tag="KPH29_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG1467285.1"
                     /translation="MSQLLIKNAYLITMNADREVYETGSILIENNIIKNVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLIRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHNPGAAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(22339..23682)
                     /locus_tag="KPH29_001686"
     CDS             complement(22339..23682)
                     /locus_tag="KPH29_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBG1467286.1"
                     /translation="MVPNTNNVQPDGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     regulatory      complement(23755..23856)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            24186..24839
                     /locus_tag="KPH29_001687"
     CDS             24186..24839
                     /locus_tag="KPH29_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBG1467287.1"
                     /translation="MTKIIKVQKPKFTGELEIIENLEDILEDIFNDEKIFNKIIENEV
                     ISDLDSVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNSFFDGGSIYRVEFKN
                     SKLIGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVF
                     EYTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDEIRGAIFDVSQAIDLTKLMGIIIK"
     gene            24980..26251
                     /gene="brnQ"
                     /locus_tag="KPH29_001688"
     CDS             24980..26251
                     /gene="brnQ"
                     /locus_tag="KPH29_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBG1467288.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAIFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            26453..27640
                     /locus_tag="KPH29_001689"
     CDS             26453..27640
                     /locus_tag="KPH29_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HBG1467289.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMTKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSVAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
     gene            complement(27870..29894)
                     /locus_tag="KPH29_001690"
     CDS             complement(27870..29894)
                     /locus_tag="KPH29_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG1467290.1"
                     /translation="MDKKKLILITYDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGEVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLELDEKLIKSPSLMEYMPKSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKRAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCVEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKGIKIIENNII"
BASE COUNT        11626 a         3418 c         5099 g         9941 t
ORIGIN      
        1 taacttttaa aaattagttt tactaattta caatagaata ggtgtgctgg tgtggctcaa
       61 cggtagagca gctgacttgt aatcagcagg ttgtaggttc gattcctatc accagctcca
      121 gtaaagacta tgtttttagc atagtctttt tttattgttt tttattgttt gatgtatttg
      181 ttattttgta ttacatactt ttagtggcct aatttataaa ttataggtat aaaagatgac
      241 acagtttaaa attattgttt taaactgtgt ctgtgagttt taaatcaatc taccatgaat
      301 caccattctg gcatcatcaa attcataggt acatgtagaa agtgtaacta ttttatcatt
      361 gctatttacc ttaataggag atttatacaa tgatttagaa gtaatatcat ttatgtaatt
      421 ttgataatca gattcattgt taaaatttgt ttttagataa tcataatcag attctacaat
      481 ataagcagaa aaaacatcat ataaaaattc ttttccattc aaggttattt ttattttatt
      541 attgttttta aagaagtcag catctttaaa cttatttagg ttgttgaaca tagttttatt
      601 tttcatatta tgtccatata ttatgatatt tttgtcatca atagatttat ttctataatc
      661 cataaacagt gtaccagaaa tagaatcttt tttataaaaa tctttgtcaa gatagtaaga
      721 attatcttta gattgtacaa ctggataatt tatatttgta ttctcaacag aaagccaaaa
      781 tttaaaatca ctgttaatct ttgataaatc ctctttttta tattcttttt tttgaagttc
      841 tgaggatgtt ttagtatcat gattgtattt ggtaagtttt gagtatatat taaatccact
      901 gtaaagtatt actaatacta gtataatgtt aattactatt ctatataatt ttttcaatat
      961 atcacctcga gttaaatgga gtttatatat ctatattaca ctatttttga ataaatttaa
     1021 atttttagta taatattact ttgacatgta aaagaatttg tgttaacata actaagtaaa
     1081 ataaatatta agtaagccag ataatcgcat gtaatcttac atgaggaaag tcggagctcc
     1141 atagagcagg gtgctaggta acgcctagtg ggagcgatcc taaggatagt gcaacagaaa
     1201 taaaccgcca ctattgtggt aaggatggaa aggcgaggta agagctcacc agcagctagg
     1261 cgactagttg gctatgtaaa ccccacctgg agcaagacca agataggaat aaagagtgct
     1321 gctcgtactc tccggtaggt aggtcgcttg agcctattgg tgacaatagg cctagaaaga
     1381 tgattatcta atacagaact ccgcttatag acttgcttaa ttatttcgat agtcaaaaac
     1441 atcagtttaa actgatgttt ttttatttgt aaatttgtct aatctacttt taaaattttg
     1501 aggaatattg taatataagt tagtacagat attaaaaatg gtataatata agatagatat
     1561 aaaaagaaaa agtaaaatga tgtaaataat atgatatgat ataagatgta gcaaatttag
     1621 taaataatta tttagatatc ataatcaaat tagcaacttt gcacaatgta aatattacaa
     1681 taaaataatt gatttaagga gtgaaggttt tgagtgaaaa taatataata agaaacactg
     1741 ttttaaatgt cacagcaagt tttttaaaac aagagtctaa aataaatgaa aagctagatg
     1801 gtgttttgga aaagaaattt gaaaaagtag aatttaatga agcaaaatat gcagagcttt
     1861 taaaatttaa tatattgttt tataagacat tagcaagaaa tacagaacca cttattggca
     1921 aatggatagt agataagtac atacctgaaa tagatgaatt agaaaaagat ttagaattaa
     1981 ctacagcaaa atgtagaaag tatgtaaata aggctatgaa agagggatta gactctctaa
     2041 aagccaatga tttaaatagt tttttagctt atgataaaat ggaactatca gagagaagaa
     2101 gacgtttaga aaaggattat aaggttttaa acctatataa agacctcctt aatatatctc
     2161 ttagaaaaat aagtttagaa aagaaagaat gtggagattt atttcttaaa aatcaagcag
     2221 atgcgaaaag agaattaaaa agagaaatta tattctgtgt aaacaaaata ttagctagca
     2281 ataaagatgt ggtacctaat aatgtggaag agaataacga acaagacaaa gtagaaataa
     2341 aagcaactga aattgaggat ttgcaattag gtaaagataa ttcagaaaat gacatgctac
     2401 taaatgaaat taaaaattca aaagtaaaac atgaatcaaa atcaaatgat atagaatcga
     2461 agtttataga taagttaaat agcttaaata atgaaagtat atctcaaaca atacacaatg
     2521 aatataagaa gttatgtgga ttagaagaac ttcattctgt tgaaggatat ggttttggca
     2581 aagaaattat taaagatttt gcttgtgcaa cggttgtatt agaattttta aagagaagaa
     2641 atagagacct tatagaaggt gcaatgagac tgactataat tggagaattt ggtcctgaaa
     2701 attttaagga atttgtggac tatgtaatta aaaataaaac agaaattagt gaagatacat
     2761 ggaataaagc acagaatcta ataaaagata actatagtga attagaaaat catgaaattg
     2821 catcaaaaag aactagaaga aataaagata tcgatattga agaatacatt tacatgataa
     2881 aaaatgcaga taaagatatt tgctttagaa gtagtatttc aatagaagat gatgctgaag
     2941 aaggggctaa agaagaagta gatagtaata atcaagatat aggtgacgtt gtagaagata
     3001 aagatactac agataaagag tacgacagca ataaagaaga tataattgaa ccagaaaata
     3061 aaaagagtaa aaagaaggct aaattatttg gatttattaa aaaagacaat gaagaagttg
     3121 aacaagaaga agagaattta aatgatattt ctcctgatat aattttagat aaaccagttg
     3181 aaaatcatca agcaaaatca gaagaaatag agcaaaatga attaaaagaa ataaaacaag
     3241 aagaaccaag tcaacatata gaagaagaac gtagtgtaaa aatagaaaaa ccaataaata
     3301 ataacttaga tgaaaaagta tcttcaaata atgaatcgaa gcttgaaaaa gaaagtaaaa
     3361 acttagaaga taaaaaagca aaagaaataa aagaagaaaa attagaaaat gaaaaatcag
     3421 tagtaatacc tattaaaaag aaagaaaata gtaataaaaa atctaaaaat tcttctaagg
     3481 acaaatatag agaaaacaag aaagaaatga aaaattatgt atcagataaa gaagattctt
     3541 tagatgatga agaggttgtt tctaagaaga gtagattaaa agaaactatt atagcagtag
     3601 ttatagtggt tattgttggt gtaggatatt ttataacagt aggcaacaat aagaaaaatg
     3661 ataaagagaa tattccaaag tcatcaactc aacaacaagc taacaataaa ttaactgagg
     3721 aagagaaaaa agcacaagct gaaaaagaga aaaaagaagc agaagaaaag gcaaaagctg
     3781 aaaaagaggc tcaagaaaaa gcacaagctg aaaagaaagc aaaagaaatg gaagcatata
     3841 aagatggtaa aggtgtatat tacactgttt atgcaggttc acttaaagtt gaaaaaacag
     3901 caaaagaaac agcaaaagaa tatgaggcta aaggtatttc ttcaacaata attcaagaaa
     3961 atggatacta taaaataaaa ataggtgatt attctcaata tggagaagca caagaaaaat
     4021 gtaatgaact agctaagaaa tctattgata catatatagc tatgtatgat aagtactatg
     4081 attataagtt agaagaatta aaagaatcag ctccttcttt gagtgctgaa gaacttaaac
     4141 aaaaatatga agatttaaga agtgagttaa agaacaaatc tggatatcgt gaatatgtta
     4201 aacatttaga taaactttat gaagaaatag ttgaaggtgc ttagagcatt ataaaaaagg
     4261 agaaactatg tctcagaata aaaagtatta aaaaaatatt atttgttttg agacatttta
     4321 ttcaccttta taaaatactt ataattctgt ttaggtggtg aaggtatgtt gaaattagga
     4381 gaaaaaatca tatatgagct ttctgatggg tttgagctta gtggcataag tagatactca
     4441 aaagaatata aaaagcttca tataacaaat ctagggaata taataatcag caaatcagat
     4501 gaagttaatg aggatacagg tgtaaaatat atatatagtt tttcagacaa taaagaaaaa
     4561 ttagaggcag ttttagaaga ttctcaaata attgtctatg gaaataactt gcctttttac
     4621 ataaaaagtg aaaattcaag taaaaatgtt acagcaaatt tgactatgaa aataactttg
     4681 gatgaatatg atttaatctg caaaagtcaa agagaattta tattctatct aaaggataat
     4741 atgactatat taagtttaga tgacaataag ttttatatgg gtggaattaa taaagataat
     4801 gagaagttta tatttatcag tgggaaaaat agatttgaaa taaattttga tgatatagaa
     4861 agatacattt tagaagataa aagagtgtca ttgaagggat actttcatat ggaaagagag
     4921 gggattatag taaggtctgt atctatattt aataacaata tagatagtgt agttccatct
     4981 gatttaaatg agagagttaa agataaccaa aaaataggaa atcttcctaa agattgtgat
     5041 atagtattct gcaagatatc aggaaatata gatggatttg attataaaaa cacaaacatg
     5101 cttttagtaa aatatcaaga ccaattaatt tttataaata agaaatctaa gaagactatt
     5161 gtaaagtcag cgaaagataa ttgttctaag ttaaatttag gagaagatat aatattatat
     5221 gataataaaa atgtatttaa cctacatata aatgataaaa atagagaaat tatgcaaata
     5281 gatgacttaa aagatataga aaatgaaata gtgggatata cattaaaaca tgctccattt
     5341 tttattcaag aagatttcga tagcttgact attctaaagt cttttcaaaa agaaatcata
     5401 tctataaaaa atagtgatat aaaagacatt gtaattaata aagaacttga aaacgaaaat
     5461 tctaattttg tagaaacgga aataaaattt aataatcaaa aagtactttt aaacttaagt
     5521 aaaaatatgg ttcaaaaact tatgcaagat gtatttatat atgcaaagca acctctactt
     5581 aaagaaaatt ctattgaagt gatatataag aattggtcta aagctatgaa tgatatgata
     5641 atatttaatt tttttggaaa tatttactat atgaaatctg aatttgataa gattttagaa
     5701 aaagagttaa atgatgaaat aagaatagaa gttataaatt cactttataa gcaaatacaa
     5761 gaacaaagaa acaatttaga tttattgtca gcatatatgc caagaatatt agaaaatcaa
     5821 gaaatagatt tgtttgaaaa gtataatact aaacttgatg tacaagtgtt taagcagatt
     5881 aaaaatctat tgtctgactt atcatataat ataagcagtt atttaaatga agttgaaaaa
     5941 agtttagaca atatcatctt tgtaatttca ggagaagata agaagaaata caattataga
     6001 atgttaaaag aaagtgaaag tgcaagtctt gatgtatttt taaaacaagc catatcaaga
     6061 ttaaaccatt tagtagaaaa tatgtatcct tattatgtag atgaaactag tagggaaatg
     6121 tttaagttat ttgagttatt gtggaaaaac tatagaaata ttgatgatga tagtataaaa
     6181 gaaatattat tcgagagaat aacaaataca tatgtattta aacaattgac attaaataat
     6241 agtacaaaag aaagaagaaa agacataatt gaaaaaatat ataactctgt tgattatgga
     6301 accaataaac tagatgaaaa tatgtttttc acaggaggaa taaagtatgt caagtaaata
     6361 caatgtttgg acttttaact atgagttctt aggattggac tcaggggatg aaaaaaataa
     6421 tgaagtttat tacgagattg atttagatga aattttaaat gaaaactata tagatgaaga
     6481 tatagatagt ggtttagatt ctgatggaaa tagtatttta ggagaattaa tatcagaaat
     6541 ggatatagat gaggatgtca gtgttgatat gacatatcaa gaactagaag ttgacttaga
     6601 agatttagat tcttatctag atgaagatat agatagtgat ataaaaggca tcttagatga
     6661 aatttagtac ctgtataagt tttggataaa atataaacaa tacaatatta acttataaaa
     6721 ctaaaggtag atgaaatttc atctacctta tattatctta tagcattaaa tattgggata
     6781 atatagagtt tgaagaaaaa gtataaatat ggtatattaa tttggtacaa tagaaaattt
     6841 acgaagaagg gtgaaaaaat gcagatgaca gttaaaaaag caataatacc agcagcaggg
     6901 ctaggaacca gatttttacc agcaacaaaa tcacagccca aagaaatgct tcctatagta
     6961 gataaaccaa ctttacagta tataatagaa gaagctatag aatcgggaat tgaagaaatt
     7021 cttataataa caggtagaaa taaaaaatct atagaagatc attttgataa atcagttgaa
     7081 ttagagttag aactagagca aaaaggtaag acagagatgt tagaaatggt aagagacata
     7141 tcaaatatgg taaatattca ttatataaga caaaaagagc caaaaggtct tggacatgca
     7201 atttactgtg caaaaagttt tataggtgat gaaccttttg ccgtattatt aggtgatgat
     7261 atagtagatt cagatgttcc gtgcttaaaa caattaatag atacttataa tgagtataaa
     7321 acaactgtat taggtgttca aaagatagct aaagaagata ctaataaata tggtatattg
     7381 gatgtaaaac atatcgaaga tagagtatat aaagttaaag atatggttga aaaacctgca
     7441 attgaagaag ctccatctga tatagctata cttggaagat atataataac tcctgcaata
     7501 tttagtatat tagagaaaca gacacctggt aaaggtggag agatacaact gacagatgca
     7561 cttcaaactc ttggaaaaca agaggctatt tatgcttata actttgaagg tagaagatat
     7621 gatgtgggag ataagattgg ttttttagag gcaacaatag actttgctct aaagagagaa
     7681 aatttaaaag atgatttaat ggactatatg agaaaaaaag taagtgaaaa ataaaatatt
     7741 agaaaagagg taattacaat ggcagtttta gtagctggag gagcaggata tataggaagc
     7801 catacagcaa tagaactttt agaatcaggt tatgaagttg ttatagttga taacttaagt
     7861 aatagcaact caatagtggt tgatagaatt aaggaattat ctaaaaaacc agtaaaattt
     7921 tataatattg atatcagaaa taaagatgaa atgcatgttg tatttaaaga aaataatatt
     7981 gaatcaatta tacactttgc agctttaaaa gcagttggtg aatcagtaga aaaaccaatt
     8041 gaatattaca gcaataatct aataagtacc ttaaatttat ttgaattaat gagagaatat
     8101 ggtgttaaaa agtttgtatt tagttcttca gctactgtat atggagaccc acatacttgt
     8161 cctatattag aggatttccc actttctgta accaatccat atggaagaac taaacttatg
     8221 atagagcaga tgttagtaga tatatcaaaa gcagataagt cattggatat agctctttta
     8281 agatacttta atccagttgg tgctcataaa agtggtagaa taggtgaaga accaaatgga
     8341 gttccgagca atctaatgcc ttatataaca aaaatagcag taggtaaatt gaaggaattg
     8401 agtgtttatg gtaatgatta tccaacacat gatggaactg gagttagaga ctatatacat
     8461 gtattagatt tagctgctgg tcatgtaaaa gcattacaaa aattagaaga aaatccagga
     8521 cttgttgttt ataatttagg aactggaaag ggttatagtg ttcttgattt agtaaaagca
     8581 ttttctaaag caagtggaaa agaaatacca tataagattg taggaagaag ggctggagat
     8641 gtagccatgt gttatgcaga ttcttcaaag gcagaaaaag agcttggatg gaaagctaag
     8701 tatgaacttg aagagatgtg tgaagattca tggagatggc aaagtatgaa tcctaatggg
     8761 tatgaagagt agaatacata cttatatgta taaatgtaac aaatttgtaa atataaggta
     8821 ttggtaattt tatgttataa ttagacacat gtagtaatgt ttctaaaaga gggggaagtt
     8881 atagtgaaaa aaagtctaaa aaagtattta gtactagcac tgactttagt tttagtattt
     8941 gcatgtggcg taccagaatc aagcgcagcc agtaaacatg tgattatagt aaattcaaga
     9001 aagaatactc taggatattt tgtaaataat aaacttgtaa aagaatttag agttgcaaca
     9061 ggtaaaaagg ggtcagaaac accaacaggg aaaacaaaag tagttaacaa gataaaaaat
     9121 agaccatatt ataaaggtaa tatacctgga ggaagtccaa gaaatccatt aggagataga
     9181 tggatgggat tagcattaaa aggaacttat ggagatacat atggaataca tggaaataat
     9241 aatgagagtt ctataggaaa gcatatttct ggtggatgta taagaatgca caataaagat
     9301 gttagatggt tatttgacca agttcctgta ggttcagatg taattataga ttatagtaat
     9361 gacagctatg taaagatagc ggcaaaatat aagataaatc taaatcaaac aggatggaaa
     9421 acagaaaatg gtaagaagta ttatgtaaaa tctgatggaa cataccaaaa gaatagttgg
     9481 ctaaaagtaa atggaaaaat gtattacttt gatgcttcag gagtaatgca aacaggatgg
     9541 aaagcaatta ataacaaaaa atattattta ggaactgatg gagcaagagt atctggatgg
     9601 aaagtaatag acggaaaaac ttattacttt aactcagatg gagtaatgca aacaggatgg
     9661 caagaaaaga atggaaagaa atactatcta ggatctgatg gtttatcagt aacaggatgg
     9721 caagaaatag atggtaataa gtattacttt gacaaaacag gaataatgca gacaggatgg
     9781 caacaaatag atggtaagtc ttactattta gacaaagatg gaaagatgct aactggttct
     9841 caaaaaatag atgggaaaga ttatactttt aatgaagatg gaactataaa tcctacatgg
     9901 gatacaatta taggtgcaaa tagatttgat acagctaaaa agataagttc agttggaaac
     9961 tggaatgctg atagttcaga tactgtaata ttagtaaatg gtaatgcaat agctgatggt
    10021 ataacagcaa ctcctttagc aagtagctat gactcaacaa tactacttac taatacagct
    10081 aatttaccaa ctgaaactgt tgaagaaatg aagttattag cacctaaaac agtaatcttg
    10141 ataggtggag aaaatgcaat aagttctaaa ttagagcaag aaataaaaac tactttcaac
    10201 acagagacaa agagaatagc aggtcaagat agatatcaaa ctgctacaag aatagctgaa
    10261 gaacttggaa atcgagaaga aataaaaact gcatatatgg tttctggaaa tggggaagca
    10321 gatgctttat ctgtagcttc taaagctggg gaagaaaaac aacctataat attagtcaat
    10381 aaagatggaa ttacagaaga atcttataaa tggctaacag aaagaaaatt agaaaatgca
    10441 tactttatag gtggaccaag tgcaattaac gattcagtaa tagcaaaaat gaatgatata
    10501 actactgaag atatttcagg aaatagaata tatggagata gtagagtaga tactaatgcc
    10561 aaagtaatag aaaaattcta tggagatact gatttacagg ctgtattagt tagtaaatca
    10621 gatgctttag tagatgcttt atcagctgga ccattagctg taaaattaca ttcaccaata
    10681 gttttaatgg ataattcagg attatcatta gagcaacaaa gagtatttgc aaataaaaaa
    10741 gttgaaactc cttatcaaat aggaggcgga gtatcttata tagtaatgga taaattaatg
    10801 gatattcttg caaaatagta aaaatatata ttgaaaaata attataattg agatgtgaaa
    10861 ttcaatcaat aaaagcttga ttgttttaca tctcaatttt ttagtataaa attaagttta
    10921 agttatataa aaatcttgga atatttttaa aactcatata aaatgaatat agatgctatc
    10981 atcatatgtt aagagggtga gtaagattat aaagaaaaaa tatataatgt ttattgatga
    11041 aagtggaaga gctgatataa atcatgaaga cccatttaca ttgacaggag taatttttga
    11101 atataaatat gcagtaagtc aaggcgatta tgtatgttct ttgaggaaag aaatggatgc
    11161 atttaaagag tattgttttg gaaccagtaa cataggaata catttgacag atatatcaag
    11221 aaaatcaaga gcatttgaga agtttgatga ttcacagatt aagttattct atgatgagtt
    11281 gcctttattt ctatctagat tagagtgtac tataatatca gtaacagtag ataaagctag
    11341 attaaagaaa tattatgcac cttcaaagga accgtatgtt gtggctttta tacatgttct
    11401 tcagaatttt tattcatttg taaataatga aactgtagaa tctgcacgaa tagttataga
    11461 gtcaagggat gatgtctcaa acttaaaagt acaaaaggcg ttttttgatg tatttaataa
    11521 tggaacaata tacttagata tagatgaaga attaagagat aaggttaagg gttttataat
    11581 tgccaaaaaa gaagatgctg actatagaga aggtttagaa atagcagata ttttatgtaa
    11641 tcctcttagc agagcaagaa gagggttaat agaagctaat ccaaagtgta tgcaatatgg
    11701 agaaaaaaac aaaatattca aatctgtcag aaacaaaata tatactccaa cgagtgtgaa
    11761 tgatataaga aattggggat ttaaagaagt tcctattgta gagcctgttg gaccatggct
    11821 tgaaaaaatg cgttagatac aattgacaaa tttaaaaaaa tctgatataa tgtaaataac
    11881 ctagttattc aggaactgaa tccagttgct gttgtataac gataattact gagactcgga
    11941 tgccgactgt cagttaggaa tctatacttt taaaagtata gattttttta ttttataaaa
    12001 agaataaaag aggatattta aaaataaccc ctttattaat aaattataat tgagcgtttt
    12061 taatgacaaa gtttttaaag tttttaattg caggaggcaa atatctatct tttataaatg
    12121 ctaagtaaaa gtttctttcc caacttgggt gaataatctg aattactttt acttttgtaa
    12181 agctaagcat attcatattt gggactacag caattccgaa attttttgca actaatccag
    12241 caattacttg gtcttcctct acttcataga ctatattagg ctgttgattt atttttttaa
    12301 acatatcatc aattatatat ctaagaccac ttctctgatt aaatactatt tgaggataag
    12361 gtatggtttc ttttaaatct atagtatcct ttgcagctag tggatgacta tatggtacaa
    12421 ttagtactaa atcttgtttg gcaacaggta taaattcaat tcccttttct ttttcaagtt
    12481 tcgaacaaaa tgcaatatca tattttcttt ctttaagacc ttgaataatg tcagtagtta
    12541 caccagtatg aaatttaaaa tctattgatt tagcaggatt agattttaaa aatttatgca
    12601 ctatgtctgg gataaaatca gtaccaagtg ttctaagaaa agctaagtct atttgtcctt
    12661 caccagtacc agttattttt aatgatttta cacttgaatc taaaatttct aatgattttt
    12721 ccacatcttt taaaaatatt tttccatact tagtaaggac tatatttcta ccttcttttt
    12781 caaatagagc caccccaagt tcattttcta gtaaagaaat agcatgactt aagcttggtt
    12841 gtgttataga taaattttct gccgcttttg tatagtgttc taaatgtgcc aatgtaacaa
    12901 aatagcgtaa gtgatataaa ttcaattttt gttcatcctt tcataggtat ttttatttat
    12961 atatttgtag aagttaacct ttaagatatt tttacatttt tattatatat gattatagag
    13021 taagaataaa agcttttaaa aaaatttatc gttttttgtc tgaattataa gataaatata
    13081 tttatacaac ctaatagaaa aatacaagaa ttatcttata aataaaatct ataaacaaaa
    13141 tctatgaatt aataggaaat tatcaatttg ttattagtaa agtctcgtga tacaataaat
    13201 ttataaaatc tagataaata gaaatcaaaa aatactttta tttttaaggt aaaataatac
    13261 tactagtagg ataaataaaa atataaaatc aaagatacta ttaattataa agaaaataaa
    13321 gggggcaaat ttaagctatg aataatcaaa aaaatcttaa tattccaatt acaattagtt
    13381 cttatacatt gggaacagaa gtttcattcc gtgatagagt tcgtattgca aaagaagctg
    13441 gatttgatgg tattggattg cgtgctgaaa attatattga tgcaaaaaat gcaggtgtta
    13501 cagatgaaga aatgattgca attcttaatg aatatgatat gaaagttaca gaagtagaat
    13561 atataactca atggggaaca gaagaagata gaactgaagc tcaaagagaa aaagagcaaa
    13621 atgtttatca tatggctcac ttatttaatg ttaaacatat aaattgtgga ttattagaga
    13681 tgataccaga agaacaaatt ataactgcat tgggagaatt atgtgacaga gcaggtgaac
    13741 ttattatagg tcttgagttt atgccatata gtggtgtacc agatttggct actgcatggc
    13801 gtgttgtgaa aggatgtaat cgtgaaaatg caatgcttat attagatact tggcactggg
    13861 ctagagcaaa gcagacagca gaggaccttg cacctgttcc agcagataag atagtatcaa
    13921 ttcaagtgtg tgatgtttta gaaactcctt atgaaaaact tagggatgaa tcactacatg
    13981 accgtttggc tccaggtgaa ggatatggaa acacagagga atttgtgaaa ataataaaag
    14041 aacacaatat atcaccaaga gtcatagggg cagaagtaat atctgattct ctaatatcta
    14101 aagggttatc cactgctgct gaaactgtat ttaatgcaac taaaaaagtt ttggataaag
    14161 catggtctga agtatctcca aaataaactt atataatata aaaatataca gagtattata
    14221 atataaaact tttaaatata aaacttgaaa ggaaggaaaa ccttatgaag attagttcta
    14281 tgtttacacc tataagaatt ggttcaatga cagttccaaa tcgttttgta gttcccccta
    14341 tgggaaacaa ttttgcaaac acagacggta ctttaagtga aacttctaag gcttattatc
    14401 ttgagcgtgc tttaggtggt tttggattaa taactataga atctagtgtt gtagacaaaa
    14461 aagcaaaagg aggtcctcgt aagccttgtt tatatgatga tagcacaata gatagtttta
    14521 aaaatgtaat agatgcttgt catgatgcag gttcgaaggt atctatacaa ttacaacatg
    14581 ctggttctga aggaaatgaa aaagtagcag gacatccatt aaaagctgct tctgcaatac
    14641 ctgcatcaaa tggtcgtaat acaccactag caataacaac agaagaaata tatgaactta
    14701 tagaatctta tggagatgct gcattacgtg cacaaaaagc tggtgcagat gctgttgaag
    14761 ttcactgcgc tcatggatat ctggttagta gttttatatc acaaagaaca aataagcgtg
    14821 tagatgagtt tggtggatgt tttgaaaata gaatgagatt acctcgtctt atcattgaaa
    14881 atatacgtaa aaaagtagga aattctctag caatattatg tcgtataaac agcactgatg
    14941 atgttccagg aggaattgat atacatgata gttcagtaat agcagcatat ctagaaggtt
    15001 gtggaataga tggacttcat gtttcacgta gtatacatat acatgatgaa tatatgtggg
    15061 ctcctacgac tttacatgct ggattcagtg cagaattagt tactgaaata aaaaaagctg
    15121 ttagtatacc agtaataaca gttggacgtt atacagagcc acagtttgct gagttaatgg
    15181 tcagacaagg aagatgtgat ttagtagcat tcggtagaca aagcttggct gacccagaaa
    15241 tgcctaataa agctaaaaat ggaaaactag acgaaatgat accatgtatt gcatgcttac
    15301 aaggatgtgt accaaatatg ttccaaggaa aacctatagc ctgtcttgca aatcctatat
    15361 taggacatga agcagagctt aagccagctg aaattagcaa agaagttctt gttgttggtg
    15421 gaggtgttgg aggtatgctt gcagcatggg tatgtgctaa aagaggacat aatgtaactt
    15481 tagttgaaaa atctgaagtt cttggtgggc aaatgcgttt agctgcatat cctccaggga
    15541 aaggagatat tactaactta gttagaagtt atatttcaaa atgtaatcaa tatggtgtaa
    15601 aaatatgtac taatacagaa gctacagtag aacttataaa ggagaaatca cctgatgtag
    15661 ttattatagc tactggagca actccacttg tacttcctat accaggaatc aatgattcag
    15721 gattaattca tgctgtagac cttcttgatg gtaaaaaatc ttgtgggaaa aaagtacttg
    15781 ttgttggagg tggaatggtt ggatgtgaag tggcagcctt ccttggagaa caagaacatg
    15841 aggttactgt tattgagctt agagaagaag ttggtgcaga tgtaatttca gaacaccgta
    15901 aattccttat gaatgatttt gaaaattata atatacatac cattactggt gcaaaagttt
    15961 ctaaatttta tgatgatggt gtcgactatt tacttacaga tggcacagag catgatttaa
    16021 aaggttttga ttcagtagta cttgctatgg gttctcgtaa ttacgaccca ttaagtgaag
    16081 ttataaaaga agttgtaaaa gaaacttata ttgttggtga tgcagtaaaa gctcgtaggg
    16141 cattagatgc aacaaaagaa gcttttgaag tagcattaaa tatataaata ataaataaat
    16201 ttttctttgt aaataactga ataggttatt tgcaaagaaa atgataatat tgggaggaaa
    16261 ttatagttat ggaaatttca ggtagaacag gattatttgc attaataggt acaccagttg
    16321 gtcactctaa atcaccagtg atgtataatt atagttttaa gaaattagat ttagattata
    16381 gatatttagc ctttgatatc acagtagata aggtaaaaga ggcactttta gcaataaaaa
    16441 cttttaatat aaaaggagcc aatgttacta tgccatgtaa atctgctgtt acagaatata
    16501 tggatgaact ttctccagca gctcgtataa ttggagcatg taatacaatt gtaaatgaca
    16561 atggaaagtt agttggtcat ataacagatg gagtaggcta tgttcgtaat ttaaaagaaa
    16621 atgggataga agttaaaggt aaaaagatta caattatggg tgctggtggt gcagcaactg
    16681 ctatacaagt acaatgtgct ttagatggag caagagaaat atctatcttt aatgctaagg
    16741 atgattttta taaaagagca gagcagacag ttgagaatat aaaaaaagat gtaccagaat
    16801 gtgttgttaa tctatatgat ttagaagata caaacaaact ttatgaggag atagaatcaa
    16861 gtgatatatt gacaaatgca actcttattg gtatgaagcc ttatgacaat gaaacaaata
    16921 taaaagatac tagtgtttta agaaaagact tagtagtaac agatgtcgtt tataatccta
    16981 agaaaacaaa aatgattgaa gatgctgaag ctaatggatg taaggctatt ggtggattag
    17041 gaatgttatt ataccaagga gcagaagctt ttaatctata tactggatta gaaatgcctg
    17101 tagaagaagt taatgaatta tgctttaaat agataagagt gatatcttaa acaatgaaaa
    17161 aacatacgta tttcaatatt tttaataata aattacaaat tgtcatagtg ggttttgcat
    17221 catagtccag ttaaatttga agctttaact gactatactt taaatccatt atgaggatga
    17281 gtttattgta cacaaaaatg ttaataaatt aacaagaatg ggagaaaaat tatgaacaat
    17341 aaaaaatatt atcctactgc catagcgttg tatttttcat attttttact tggtattgga
    17401 atttcaattt taggacaata caaaccagaa ttttcatcaa tgtggggggc taaaacacta
    17461 tcagatggaa cattggatgt aagtattgtt ttagctgtta ttgctgcact aggattaggg
    17521 agacttatat cttatccatt tgcaggacct atttcagata agtatggaag aaaagtaagt
    17581 ggtcttatag gaaactttct acatgcaatt ttctttgttg gaattgtatt ttcaccaaat
    17641 ttttatatag catatgtatt tgcaataatt ggaggggcag caaattcatt cttagatacc
    17701 tgtgtaactc cttcttgtat ggagatattt gctagtttag gaacaatagc aaatatgttt
    17761 actaaattta caatagcttt agcacaattt ttgcttcctt ttataattgg atttgttgca
    17821 gcaaattcaa tatcatttaa agttattttt atagtaacag cgattcttat tgtagtagat
    17881 gccattttga tagctgtgtt accatttcca ccagctaata atgttattga taacaaaggc
    17941 aaaacagtta aatcagagaa gatgaaattt acaccaacaa gtatagctct tgtttgtatt
    18001 ggatttacat gtacatctac atttgtatta tggttaaatt gtaatcagga attgggtaaa
    18061 ttgtatggga tggctgaccc tacaaaaatt caatcctttt actcaatggg agtaatatgt
    18121 gcagtattaa taacttcttt acttattaaa aaatatatta aaccaataag aatattagtt
    18181 atatatccta taatagcttt acttatgcta ttaattgtat attttgttca aactccaact
    18241 atttgtatgt tagggggatt tgtaataggt tattcagcag caggaggtgt tcttcagtta
    18301 acaacgtcaa ctgcaaatga aatgtttcca actaataaag gaaaaataac atctatagtt
    18361 atgattgcat ctagtatagc aaactatgtt attttaaata tagcaggtat tattacaaaa
    18421 tcagggggag ttaatggacc aaaatatgtt gtattgttta atgtggcaat aacatttgtt
    18481 ggaattttac ttgcattgtt tgtaaacatg agatatgaaa aagaaaaagt gtatgattat
    18541 gatgtctaat attagtataa tatatgcata gagaagtcta aatctaggat agatttttat
    18601 taaaagtaat taaaattaat aaggatatta tttacagtta taagataggg tatttacata
    18661 attaataata aggatattac tagaatttca atttctagta atattttttt gcgtataaat
    18721 gtaaacttga cgaaataagt agtataatca atataaatta catcatgtaa aggaggataa
    18781 tttggtggag aatgttttta agcgattgca ggaatttaat ggttatgatg gatacaaaga
    18841 atcatttgaa atgaattacc tatgtatata tgaaagtata ccgttaagag aacaagtaga
    18901 acttgcaaat aatctagtag atgaaatttt aaatatgtat aaaagtgaat ctaatgaaat
    18961 ttatttattg gaggatagta attctaagtc tttaatttgt tatttcgaaa tttttatgaa
    19021 aaagataaat actttggtaa aagaaatgat tattgatgag aaatggttat ataaattaac
    19081 taaagaatta atatataaga gtaaaaaagt tgagtatgta aaactagggt tagttttaag
    19141 tgagaaatat ctaaatgtag aaaacttaag agaagttgta gatacatttt ctaaatctgg
    19201 agaatacgta ttttatctga gtaatactat taaaaaactt gaattttaca atacatattt
    19261 atttaattta tctaaaaagg ctacaggttc aataaaagta tttgctattg ttaatatgga
    19321 aaatttagat tctaaaataa attcctatct aattgaagat ggttataagg atacaaagta
    19381 cgaacgatta ttgatgaact atattatttc aattgtagat ttgaatgaat atcttgaaaa
    19441 acgagattta gataaagaaa agataaataa tttagcacgt cttatatgca attacttgct
    19501 aagtgtagaa tttaaatata taggtaataa attagaatta gtgaatcgat ttttacctac
    19561 tgtagtaaat tatgggacta actttgaaag tttatattca attttcctaa tagctataaa
    19621 cgtcttaaaa gatgaaaata tagagtacaa taagattgaa tttgaaaaag aaataaatga
    19681 tatcttgctc agtgaaaaat ggaaaaatat atattttgaa gcattaagag atgctagtgg
    19741 aaaaacagaa gatattataa aaatgtcaga aatttatgat gtaaacttat cttttgatga
    19801 cttgttgcca tatctgaata gagatattag agattttgag gtatattggc atatatctaa
    19861 aaagggaacg acaagttcta gattaaaatt attaaatttt tttgaagaga cgtttaaaat
    19921 agatgattta attggaaaaa tgaaagatat agaaaaagat aaattgactc aagaatatta
    19981 tgatgatatg ttatttttta ttgtgcttaa aggaagtaaa tcactatatc ctgaaggaaa
    20041 gaatatatct ttaaaaggaa tatttggaaa tataaatgaa gtaagaaaag aatccataaa
    20101 tatcttaaaa agatacagag agaaactatc tcttgaagaa ttaaaaattg ttaaagaagc
    20161 gtatgaaaaa gaaaaaaatg ttatattgaa ggatgaatta agaagagtat tatatgaaag
    20221 taataattta aaaaaagaat ttgtaaatat tgaaaaaata aaagtagatg aacatggtaa
    20281 ggatatctat ttgacttcaa tagctgttgc tggttcaagg tttagaaata gagaatatct
    20341 tgaaaaagaa ttggagaaga gtaagatata ctatttaact agagagaaag ataatctata
    20401 tgatgaaaaa gctattaaaa tagttggtga aactggatat gtaataggct atgtcccaag
    20461 aaaagaaaac tatattttga gcaatttatt agatggagga aaactgctgt attgtagagt
    20521 aactgaatat aatttatatg aagattgtat ctacgcaaat gtgtacttga gttataaaga
    20581 tgtaattgaa acagttgaaa actctttaaa gatggtttta gataagagta gaataaaact
    20641 tataaactaa gacgtttata tattgcttag atgatgtaat cctaactgat tggttatttt
    20701 ttctggaatt atggtgtatt ttatgagcaa tgttaatgaa tataaaataa aaaaacaatt
    20761 gttttgtata tatgatgtgt ataaatatat ttatatattc atatttacaa aacaattgaa
    20821 atttatgtta ttttatgatt ttaaatttag atggaatttt aacttctgct ttatctttta
    20881 ttttagaagc ttgtttttta actttattta ataattcaga ttcatctaca actaatactt
    20941 ttctttcctt catcaaccat ttaccatcac aaatagtaga atctacattt tcactagtca
    21001 tagaataaac tatatttcca attggattat gcattggtaa acaatgaatt gtatctggat
    21061 ttagaataat catatcagct ttttttccta cttcaagact accaatttct ttttctagta
    21121 aagcacattt tgctccattt atagtagcca tttctaagac ttcttcagct ggtacgacag
    21181 ttgggtttaa agttctacct ttatgtatta gtgatgtaag atacatatcc ctcataaggt
    21241 ccattctatt atttgaagga gctccatcag taccaattga aacaggtatt cctttttcta
    21301 acatttcagg aattgaggca aatcctagaa ctactttcat agctgcacct ggattatgag
    21361 aaacttttac atcatgtaat ctaaataaat caatttcttt atgagtaagc caaactgtgt
    21421 gagcagctaa aagatttggt cctaatacac ctagtttatc aagatattca actgttccaa
    21481 ctccgttgtt ttgctttaca aatgaaattt catcagctat ttcagcaata tgcatatgta
    21541 tacctgtatt gtatttatca gctaactctt tagtcctaat aagcaattct tcagttgtat
    21601 taaaaatagt tcttaatgca aaccatattt taatcctctc attagatgtg ttattaaact
    21661 tatggaataa gtctaattga gtgttgattt cctcatcagt agttttttgc catgcttcag
    21721 gaagaccatc tcctgtatcc ataactgatt tagctaaaca ggctctaagt ccagttttat
    21781 caacagcttc aaccatagca tctacatatt gaccaccaga ttctaaaaat gtagttaccc
    21841 cagattttat caattcaaca caacatgcag tagatgaaat caatgaatct tcatagttga
    21901 agctactttc gtatggccag attctctctc taagccaagt taaaagtaca acatcatctg
    21961 caagaccacg tcctaattgt tgggataagt gaacgtgtgt attgactaac cctggcatta
    22021 gtatttttcc tttagcatca tatatctcag catctttatc tacaatatta atatcaaact
    22081 taccaacatt ttttataata ttattttcaa taagtatact tccagtttca tatacctctc
    22141 tatctgcatt catagtaatt agatatgcat tttttataag taattgtgac ataagtacaa
    22201 cctcttttct atatattttt atatctatgt attatgataa taaattttct atacaaagtt
    22261 aatagcaaaa tcaatttata cataaattga ttttgctatt tataaattta aaatattgac
    22321 ctagaactat ttaaaatatt aacttagaac taagtttaca actgcaagta aacctataat
    22381 ccaaagtgta atactgattt tatcagtttc tttagctacc acatgcatta ttatatatga
    22441 aataaatcca aagcataaac caatacttat actataagtt aaaggcatca ataaaactgt
    22501 aacaaaagca ggtacagcta ttttgctatc agcaaaatca atctgtttta tgctttcaaa
    22561 catgaataca ccaaccatta caagtgcagg agctgcagcg taagtaggaa ctataccaag
    22621 caatggtgta aatataagtg tagctaagaa taatgctgct gttactattg aagcaagtcc
    22681 tgttcttgca cctgctgcga taccagaagc agattctcca aatgttgtaa cagtactagt
    22741 tcctaagaat gagcctatta aagtagaaga aacatctgca tataacattc ttcctaaatc
    22801 ttttatttca ccattctcat cttcaagacc catttgttta caacaagtaa taagaaatgc
    22861 taatgaatca aacatgtcta taaacatgaa agagaatata ggtccaatta atgagaattt
    22921 caatgcacct acaatatcta atttaaaagc aactggtgct atactaggag gtgttgagat
    22981 tattgctgtt ggcaactcca caaatccaaa tataacagat attattgttg caactactat
    23041 acttatcaat attccacctt taactttttt atattctagt attgccatta agataagagc
    23101 aaaaagagct attaaaactg ttggtgtaaa atctgcaagt gaaactatag tatctggatt
    23161 agctattata acacccatat tttttaaacc aacaaaagca aggaaaagac ctatacctgc
    23221 tgaagatgct actgccaatt ctctaggaat tgcgtctgca attgtctttc gaagacctgt
    23281 tactgcgagt gtaaagaaga cacatccaga tagaaaaact accccaagac ctgtttgcca
    23341 tgggattcct gcacctataa ccaaagagta tgtgaaaaat gcatttaatc ccataccagg
    23401 agctagagca aatggtgcat ttcctaaaat cgcaaatata agtgtaccaa tggctgtagc
    23461 tagtatggta actgttataa gggcggcttt atccattcca gcttcagata atattgctgg
    23521 atttacgaaa actatgtatg ccattgttaa gaatgtagta actccaccta ttacttctgt
    23581 tttaaggcta gagccacgtt cgctaaattt aaacattttg tctaaaaagc ttaatgatga
    23641 attatttcca tctggttgta cattgttagt gtttggtacc atttttacct ccaaaaaact
    23701 ttaagatata aacaaaaaaa tatatattca tatagataga atagaacata tattcaaagt
    23761 tgatattcta ttcatagaaa acccatttaa ggtgagtttg tagaaactat tagaccgtat
    23821 tactaatatt atatgaacat tttctatttg tttttctgtt gtattatatc gtaatattgt
    23881 attatttaca ataaatataa taaaattatt cgtttttttg tcgattttat aaaaaattaa
    23941 ggttatttat tggcaagaat tactgttttc aataattgaa tatgtataag tataatgtca
    24001 attattctaa cttgttttat actattatat agaagtctat tataaatgag tttgtgatat
    24061 aattataaaa gtactgaata cacggatatt tcgtaaaaac tttttaatat atcaaatatg
    24121 tatattaagt taatatatta aaaaaaataa tatcaaaaat aatataattt atagtggagg
    24181 ggaaaatgac taaaattatt aaagtgcaaa aacctaaatt tacaggagaa ttagaaatta
    24241 ttgaaaatct tgaagatata cttgaagata tatttaatga tgaaaaaata tttaataaaa
    24301 ttatagaaaa tgaagttata agtgatttag attctgtaag agttagcttt gaatcttgta
    24361 tatttagaaa tgtatccttt gaaaattgca atttaaaaaa gattgattta cttgatgtgg
    24421 tatttgaaaa ctgcaattta tcaaattcat tttttgatgg aggaagtata tacagagttg
    24481 aatttaaaaa tagtaaattg ataggagtta gatttgatga ttgtatactt aaaaacgttt
    24541 tgttcaagga tgttttaggt aagtattcta acttttcatt ctctaaattt aagatagttt
    24601 ctatcttaga ttcaaatttt gaaaattctg tatttcaaga agtaaaaggt gacaatctag
    24661 tatttgagta tacaaactta aaaaaagcaa actttaatgg aactaaactt tctggcatag
    24721 attttaccac taataatatt gagggaattg agattggtat agatgagatt agaggagcaa
    24781 tttttgatgt gtcacaagca attgatttaa cgaaattgat gggaattatc ataaaatgaa
    24841 aaaaatattc ataaaataaa ttgggaaaac gttaacaatg tgtgaatttt taccagattg
    24901 acatttttct gaaaataaag taagattatc ttaatattca aaatattttc aaagggggta
    24961 atttagtgtg agtaagcaga tgagagatat ttttatagtt ggttttgctt tattttctat
    25021 gttctttggt gcaggaaatc ttatgtttcc tccattttta gggatggaat ccggcaaaga
    25081 ctggttaatt cctcttattg gatttgttgt tgcagatgct ggtatagcaa tgcttgttat
    25141 tatagcaaca gcaagatgca agggaagcat ggatgatgta cttaggagag ctggtaaagg
    25201 gttagcaaga gtaatgagtg tagctgctct agcatgttta gcacttcttg taataccaag
    25261 aacttgtgca acaacatatg aaatgggtat tatgcctata tttggagacc atggaccagt
    25321 agctaaagct atattttcaa taatcttctt tgggttaact ttagcattta ctattaggtc
    25381 ttctaaggtt atagacataa taggtaagta tttaactcca gcgttattat tagtattatt
    25441 tgttttaata gtaaagggta taatttctcc agcaggacct atgagtcctg agcatatgat
    25501 agataaaaat ctatttggtg aaggtatatc acaaggttat caaacaatgg acgctctagg
    25561 tgcagcatct atggcaacta taatgttgat gtctataata gctaaaggat atacatcaga
    25621 taaagaccaa ataggaatga ctattaaagc tggttttgta gcctgtgtat ttctagcagt
    25681 agtatatggt ggtcttactt atttaggtgc aacagtttca actttatatg atactagtat
    25741 acctcaagca tctttattag ttgaaataac tagtatattg ttatatggtc aagtaggaaa
    25801 ggtaatttta ggtgtaattg tggcacttgc ctgtctgact acttctacag gtttaacagc
    25861 atcaatatca acttattttg aaggaataag taaggcgagt tataaacaac tggttatagg
    25921 tatatgtgta gctagtatgc ttatatcaaa tttaggtgtt gattctataa tagctatatc
    25981 agttccaatc ctacaaacta tatatcctgt tttattagca atagttgtta tggagttggc
    26041 tggtaaacat ataaagaatg ataatgcttt taaaggtgca gcatatgtta cattagtgat
    26101 tagtctatta tcagctataa atggaatgac aggagctgtt ccatttatac aaagcttgcc
    26161 actcgcaggt ttgggcttta actgggtaat accagctata ttgggtggaa tagttggtaa
    26221 ctttataaaa agcaacaaac aagtagcata ataatttgta attaatttaa ttttataata
    26281 tattaatttt tagtactatg ggtaggttta atctaaacct aatctaccca tagtactttt
    26341 tagattggat tggaatagca tgtatataat tttttataat ataatttttc tttgaagtat
    26401 gataaaatta gtaatagtga taagaaagta caaatttagg aggttttaat taatgcttag
    26461 aaaaataaca gcatttatgc tatccatatg tttaattatt ggacttacag gttgtagttt
    26521 acttgtaggc aagaaagata aagagaaaat tatagatgat tttaaagttg cagtggtaac
    26581 tcaaccatta tctgaaaata aagtacaata caatatggtg gaagagatga caaaagaata
    26641 tgaggaagag aataaaattg ataaagacaa agatggtcaa actaaagtta aacaaactat
    26701 aaaacatgtt gtattgccag agaattttac ttctaatata gatagtgcta ttaataagat
    26761 agtaaagttg gctgacgata aagaagttca ggctattgta gtttcaactg accaagcagg
    26821 gcttttacct gctttacaaa aggtaaagga aaagcgtcca gaaataataa ctatttctgc
    26881 ccctatggga gatgataaaa accaattaag ccaatttgtt gatgtgaatc taggggttag
    26941 tgcagaggaa agaggaaaag tattagctga acgttcaaaa gaaatgggag caaaagcttt
    27001 tattcattat gcctctacag atgatttgaa agatgttaat atagccaaaa gattagaaat
    27061 gattaaagag acttgtaaaa atataggatt gccatttgta caagttaata ctccaaatat
    27121 aaatactgaa gaagacaaaa acaaagttaa gcagttttta aatgaagata ttgaaaaaca
    27181 agttaaaaaa tatggaaaag atataaatgt atttggtgta aatgagtata tggatgaagt
    27241 tatattgact aaggctttag aattaaagta tatagttgca gagcaaagca atccatcacc
    27301 aatacaaacg tatcctagtg ttatgggtct aaaaatatct gaaaaagatg ctcaaaatta
    27361 tgataaaata aacgacatga taagtgaaaa ggctaaggct tttggtatgt caaatagact
    27421 tggtggatat cctatgccaa tggatgcttt tctaccatca gtagcaattt atcttgctac
    27481 tgaaatggtg aaacaagatt taacacaaga agatgtatgt gacccagatt atttagaggc
    27541 gtttacagaa ttaagatttg gaatcggttc agaatttact cctttaacag aggtacttta
    27601 taattatcaa tcagtaattt taagccaatt aatttattaa tattaaggaa gtgtctcaaa
    27661 atgaactttt tagtttatga ggcactattt ttctgttaaa tcaaatatat ttttatcttt
    27721 ttaacaatta aaaagaagct tatccctatg ttgagatagc ttctttttta atataaataa
    27781 gttttattat tttataaaaa atttatagaa aaataaaaaa ttataattat atataaattt
    27841 tgataaagta aagatgaatt actataggtt taaattatat tattttctat tatttttatg
    27901 cctttctcag ttattgatga gccttttctt cctttactag aaattaagta tccattgcgt
    27961 gtcaaatatt ctagcatatt tcttacttcg tattctgtga cattatattc cataaatttc
    28021 attaactttg tcctccccat tggtttcatg tgcaatgatt ttaggataga aatgcatatg
    28081 tttttgtcat attggtttaa atcattgaat atagacatat tacttgacat atgattatta
    28141 tttaattttg aagatatgtt ttgaggtaaa tcatttattg taagataatt ggaacccatg
    28201 taagccatat attcaacaca atttctcagt tctctaatat tccccatcca agtgtatgaa
    28261 tttaaagctt ctaaaagact cttatcgagt tttatgtttt tatagggaag ttcttttaaa
    28321 aatgattcta aaagaagagg tatatcttct aatctctttc taagaggagg taaatctaaa
    28381 gtgaacatac ttatcctata aaataaatcc atcctaaatt tattttctat aattaatttt
    28441 tctaaatctt tatttgtggc agctaatatt ctcacatcaa ttggaataat acttgtacca
    28501 ccaatttttc taatttcttt ttcttgtaag actcttaaaa gtttaacctg taaatcatgt
    28561 ggcatatctc ctatctcatc taaaaatata gtgccagtat gagctaattc gaataatcct
    28621 ttttttccac ctttttttgc tccagtaaaa gaaccatctt catatccaaa tagttcactt
    28681 tcaagtaatt cacttggtaa tgaagcacaa tttattgcta caaaaggttt attttttcta
    28741 tttgaatgat tgtgtattga ctgagtaaag gcttctttac ctgttcctgt ttcacctgtt
    28801 attaatatag gattatctgt aagagccatt ttttttgccc ttttaataca atcttttata
    28861 atagaactat ttccaataat attattaaat ttatatttag ctacataacc tcttttgaca
    28921 gaatcacttc ttattttttg ttctaatatt tctatattgt ttacatcttt taatgtaatt
    28981 aagcaaccct ctatattttt ataaagataa aatggttttt tagaaagtat gaatttttta
    29041 tctatttcgt ctatgtatat aatcatatta tcgatattta agttctttgt aattttatct
    29101 aagaaactct ttggcatata ttccattaag gaaggtgatt ttataagttt ttcatctagt
    29161 tccaacatat taagtaaaga cttatttaca aaagtaacct tgttaaaggt gttgaaaaat
    29221 attagaccat cacctatttc atcaatggtc atgtaaagta tagtttttaa tttagatata
    29281 ttatctaatg acgtatttaa gaaatcatga cttagtgtct ccttggacaa tttgtatagt
    29341 ttttctataa atttctcatt ttttagtttt aatactacta ataatgacat atatgtatct
    29401 aatgagattt ttctccaacc taaatcaaca atatttttta tgtattttgg tataaatcta
    29461 atctgacctg gtgttatagc tgtatctaaa tcatatattt catcacaatc tggatatgtc
    29521 ggtacaaaat ctatatgatt tataccaatt ttatatagat aagtagccat ctcaaatgct
    29581 gaagttttat tggttgtcat aagtaacgat tttgtacctg gaggtagttt catcattttt
    29641 tcatatccaa gttttgaaag tgctttcgtt ccatgaataa tttcaatatc ttccttaaaa
    29701 tgttttatta aaaaattact agttaaaggt gatagagaaa gcactacctc tccctctaaa
    29761 ttttctttta ttccatcaag tatattttgt gtttctatga ttatttcgtc tccaaaaaag
    29821 tttgttaatt cttctttgta gtggtcacta tttaatttat catatgttat aaggattaac
    29881 ttctttttat ccattttatt tctcctaaca aaatagtatt tatttttaaa gtatttaaag
    29941 tataaggtaa gttatattat acaacaaata gtagtataaa tttatatatt tgattaaata
    30001 ataggttttt ttaggatttt atcctataaa aacctatttt aaaattattt ttgcaagata
    30061 aggaaaatag ctttttatat tgta
//
LOCUS       DAELXX010000039        29888 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.039, whole genome shotgun sequence.
ACCESSION   DAELXX010000039 DAELXX010000000
VERSION     DAELXX010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29888
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.039"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1208
                     /locus_tag="KPH29_001691"
     CDS             <1..1208
                     /locus_tag="KPH29_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG1467291.1"
                     /translation="TEDLIIDKLLDKIFRDKKYNLNYDEKYYLKELIKLKTKDKKATI
                     KNIDKNKLQDIMFEFFREIDKKYNSNFLEDKEFFNLFYLHIACLIERIKKNHKIINPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     NAPVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKDKAKYK
                     DILDSMSKCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDIIYENRVPKIIFMISLIKGNLELHKQISKYLSKIMANKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            1211..2254
                     /gene="ypdE"
                     /locus_tag="KPH29_001692"
     CDS             1211..2254
                     /gene="ypdE"
                     /locus_tag="KPH29_001692"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBG1467292.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNQKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            2269..2574
                     /locus_tag="KPH29_001693"
     CDS             2269..2574
                     /locus_tag="KPH29_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG1467293.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPTMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            2671..3006
                     /locus_tag="KPH29_001694"
     CDS             2671..3006
                     /locus_tag="KPH29_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG1467294.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            3038..4360
                     /locus_tag="KPH29_001695"
     CDS             3038..4360
                     /locus_tag="KPH29_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG1467295.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILNNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEDLLDDLGLDF"
     gene            4370..5440
                     /locus_tag="KPH29_001696"
     CDS             4370..5440
                     /locus_tag="KPH29_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG1467296.1"
                     /translation="MNNTIERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSI
                     QEYLSLKNEGFEINLITNELWKLRAIKSKEEIELIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKH
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            5445..6632
                     /locus_tag="KPH29_001697"
     CDS             5445..6632
                     /locus_tag="KPH29_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG1467297.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVANNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(6829..7701)
                     /locus_tag="KPH29_001698"
     CDS             complement(6829..7701)
                     /locus_tag="KPH29_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBG1467298.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVSYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYKQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLHLNPEVFKNDALIYTNIDEVSIYNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
     gene            complement(8095..8286)
                     /locus_tag="KPH29_001699"
     CDS             complement(8095..8286)
                     /locus_tag="KPH29_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467299.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            8506..9552
                     /gene="cobT"
                     /locus_tag="KPH29_001700"
     CDS             8506..9552
                     /gene="cobT"
                     /locus_tag="KPH29_001700"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBG1467300.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            9584..10144
                     /gene="cobU"
                     /locus_tag="KPH29_001701"
     CDS             9584..10144
                     /gene="cobU"
                     /locus_tag="KPH29_001701"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBG1467301.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            10154..10927
                     /gene="cobS"
                     /locus_tag="KPH29_001702"
     CDS             10154..10927
                     /gene="cobS"
                     /locus_tag="KPH29_001702"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBG1467302.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWIIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            10947..11561
                     /locus_tag="KPH29_001703"
     CDS             10947..11561
                     /locus_tag="KPH29_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBG1467303.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENDSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      11628..11807
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            11937..13436
                     /locus_tag="KPH29_001704"
     CDS             11937..13436
                     /locus_tag="KPH29_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HBG1467304.1"
                     /translation="MLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMALNSFIT
                     KEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSSKYHDY
                     KLELVGVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPVIIVGD
                     IDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKVPVIGV
                     VPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVSIRYVD
                     YGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQMLGKK
                     LKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGKKVKGY
                     EIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEISFTRAI
                     LNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            13475..14851
                     /locus_tag="KPH29_001705"
     CDS             13475..14851
                     /locus_tag="KPH29_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HBG1467305.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDASVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            14852..15823
                     /locus_tag="KPH29_001706"
     CDS             14852..15823
                     /locus_tag="KPH29_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBG1467306.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKFGGFILWFITVGITYLTTYAIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYVSSFVSMLVFTLIHVLCRMILINLII"
     gene            15855..16925
                     /locus_tag="KPH29_001707"
     CDS             15855..16925
                     /locus_tag="KPH29_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG1467307.1"
                     /translation="MKDLGHGANVGEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIGINPDFIIPGNGATEVIYLLMKSVKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLGKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEERKYML
                     QELRNIRNIKVYDTDANFILIRAYKKTTKELKKDLLKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            16927..17814
                     /locus_tag="KPH29_001708"
     CDS             16927..17814
                     /locus_tag="KPH29_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBG1467308.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSEVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVIILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKEYLNEIIKISKKYGAYGVNIAHSGTVVGILIDKSMNDKKMIDTLCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            17802..18434
                     /locus_tag="KPH29_001709"
     CDS             17802..18434
                     /locus_tag="KPH29_001709"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003724727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HBG1467309.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            18443..19669
                     /locus_tag="KPH29_001710"
     CDS             18443..19669
                     /locus_tag="KPH29_001710"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HBG1467310.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDSLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDEGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCKERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            19666..20274
                     /locus_tag="KPH29_001711"
     CDS             19666..20274
                     /locus_tag="KPH29_001711"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HBG1467311.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNVVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKIPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRMDNFDMNVVVILDEE"
     gene            20264..20839
                     /locus_tag="KPH29_001712"
     CDS             20264..20839
                     /locus_tag="KPH29_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HBG1467312.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNIKKFNLKNIEVIKGYAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            20832..21584
                     /locus_tag="KPH29_001713"
     CDS             20832..21584
                     /locus_tag="KPH29_001713"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HBG1467313.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            21568..22689
                     /gene="cbiG"
                     /locus_tag="KPH29_001714"
     CDS             21568..22689
                     /gene="cbiG"
                     /locus_tag="KPH29_001714"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HBG1467314.1"
                     /translation="MNIEGNIAIICITENGKNLAVKINSLVNNCVIYQVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIIFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYVDEGYQVDTRGFKLIESKDELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFEHCYSTKKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSIDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPIAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            22686..23411
                     /gene="cobJ"
                     /locus_tag="KPH29_001715"
     CDS             22686..23411
                     /gene="cobJ"
                     /locus_tag="KPH29_001715"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011701973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HBG1467315.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     GKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKGGDTPVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            23408..24160
                     /gene="cobK"
                     /locus_tag="KPH29_001716"
     CDS             23408..24160
                     /gene="cobK"
                     /locus_tag="KPH29_001716"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HBG1467316.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIVAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            24179..25657
                     /locus_tag="KPH29_001717"
     CDS             24179..25657
                     /locus_tag="KPH29_001717"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HBG1467317.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEEDGFDVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            25687..26592
                     /gene="hemC"
                     /locus_tag="KPH29_001718"
     CDS             25687..26592
                     /gene="hemC"
                     /locus_tag="KPH29_001718"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBG1467318.1"
                     /translation="MNIVVGTRGSTLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSTIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            26582..28081
                     /gene="cobA"
                     /locus_tag="KPH29_001719"
     CDS             26582..28081
                     /gene="cobA"
                     /locus_tag="KPH29_001719"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBG1467319.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEDIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDNDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEDKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            28098..29063
                     /gene="hemB"
                     /locus_tag="KPH29_001720"
     CDS             28098..29063
                     /gene="hemB"
                     /locus_tag="KPH29_001720"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBG1467320.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     NSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            29216..29794
                     /locus_tag="KPH29_001721"
     CDS             29216..29794
                     /locus_tag="KPH29_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HBG1467321.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKIIKDDYKEIYIKDASVIIAATNNRELNMEIGLYCRAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEERYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNMLVTLDLEELKKFDI"
BASE COUNT        12063 a         3001 c         5538 g         9286 t
ORIGIN      
        1 atacagaaga tttaattata gataaattat tagataaaat ttttagagat aaaaaatata
       61 accttaatta tgatgaaaag tattatttaa aggagcttat aaaactaaag actaaagata
      121 agaaagccac tataaagaat attgataaga ataaacttca agatattatg ttcgaatttt
      181 ttagagagat agataaaaaa tataactcta attttttaga agataaagag ttttttaact
      241 tattttactt acatattgca tgtttaatag aacgtataaa gaagaaccac aagataataa
      301 atcctttttc agttaaaata agtcaacagt atccaactgt ttttaattta tctattcaat
      361 tttcaaaaat catagaaaat gagtatcata taaaaataag tcaagatgaa atagggttta
      421 tagctactca cattgctgtt ccttttgaaa agagagaaga agctaatttt aataaaaaat
      481 ataaaatagc aataatatgc tcttctggtg gaggtagtgc atttttgata aatttaagac
      541 ttaaagaaat ttttccaaat gctgagataa gaaatttttc attactagaa gaaaaagctg
      601 taatagaatt tgcaccagat ttgatttttt caataacaaa tcttttattt gaaataaatg
      661 ctccagttat acttataaat gaaattttag acgaattgga ttatttagat attaaagaaa
      721 gtgttagatt tgcagaccat ataggaaata tatcaagccc aaaacaatat atattaggtc
      781 tatttgataa gaatcacttt aggtgtataa aagataaagc taagtataag gatatccttg
      841 atagtatgtc aaaatgtata gttgacgagg gagcttgttc tccaacctat cccaaagatg
      901 tatgggaaag agaaagctat ttgagtacta tttatacaaa tggggtggct atacctcatc
      961 caatcgagat gacaggaaac aaaaatataa tatcagtagc tttgatacaa actgatatta
     1021 tttatgaaaa tagggtgccc aaaattattt tcatgatttc attaataaag ggaaatttag
     1081 aacttcataa gcaaatatct aaatatttaa gtaagattat ggcaaataag gatatggtag
     1141 acatgctaaa taagtcacaa tcttatgaag agtttatgta taagttaaaa atctatctgg
     1201 gaggttagac atgaatatag agttttttaa gaggttgtca gaagcagatg gtatagcatc
     1261 taatgaagaa gaggttagac atgttttatt ggaagaacta aaggcttata gtgataaaat
     1321 tatctgtgat gggctaggga gtatcatctt ttctaaaata aaagatgaga gtgcaccaaa
     1381 cgtaatgatt tgtgctcata tggatgaagt tggatttatg gtgagaagta tagacaaact
     1441 tggtatgatt cacttaataa caataggagg agtaaaacct cttgcacagt ttgtacaaaa
     1501 agttagaatt acaacaaaag aaggaaagaa aatccctgct gtcataaatg ctacatataa
     1561 taatggtaag gctgaaaata tatatgcaga tattggagca tatacagaag aagatgtata
     1621 taacctaggg attaatgttg gggatatggt aacatataca acaagttttg aggaatttac
     1681 tttaccagac cgattagtgg gaaaggcttt tgatgatagg ataggatgtt ttgtgatggg
     1741 agaagtttta aaagaactta ggaaagaaaa tctaaattgt aatattcact ttgcggctac
     1801 aagtagtgaa gaggttggaa taaggggagc aaagacatca acacagttaa taaatccaga
     1861 tatagtattt gtaatagatg tagcatgtgc taaaaatgag tttgtgagag actatacaaa
     1921 tcagaaacaa attgataaag gtgtaatgct aatgcataga gataggactc tagtccctag
     1981 tagaaaaatg atagattatg taatggaaat tgctgataaa aataatatac cacttcaaca
     2041 tgatatgttt gaatcaggtg ggactgatgg aggagaagca catctcgtaa atgaagggaa
     2101 accttgtgta gtaacttgtg taccagttag atatggtcat tgtgcatttt caatagtaag
     2161 taataaagat ttagagaaca ttataagatt gtatacacag ttaattttaa attttgatga
     2221 agaacaatat aaacatataa gaaattttat ctaaaggaga ataagaaaat gtcaacagaa
     2281 gtacaaataa tggagcttat atcaactgca ggagagagta aatcgaaagc ttttgaggct
     2341 ttaaaaaaag taaagtcaaa agattttaaa ggagcaaaat tgttaataga ggaggctcgt
     2401 aaaatagata ttgaggctca cagaatacag acagaattaa taactcaaga tttaaatgaa
     2461 gatgttgaaa gcccaacaat gagtctttta atggtacatg ctcaagacca ctatatgact
     2521 tctcagctag caaaagactt gatagaggct ttgatatatt gtctcgataa gtaattgtaa
     2581 tgtagaaata gattatttat gtaaaaattc ttttaaaagt ttagatgctt ttaagagata
     2641 ataaaaatac aaactttagg aggaattatt atgaaaatat tattatgttg tgcaggtgga
     2701 ttttctacta atatgttaat gcaaaatatg aaaaaggtgg taaaagagag tgctaaactc
     2761 aatgaaaaag attttgagtt tacagctata ccagcagatt caatagaaag tgaagttgat
     2821 gattgggata tagtacttat aggtcctcaa atagctcata aaatagactt cttaaaagga
     2881 atattagagc caaaaaataa gccttttgca gtaatagata aagatgtata tggaaaaatg
     2941 gatggagcta cagttatgaa gttggcatta ataacttata agaaacatca attagctcaa
     3001 ggataataaa aataatgagg gaaaagggag attagatatg ttttcaaaat ttgaagcatt
     3061 tatgaataga gtatttatgc ctttagctca taaggttgac aatcaacgtc accttggttc
     3121 tataaaggct ggtatggttg ctatgacacc attcacaata ttaggaagta tatttgcaat
     3181 acttccagca cttccaaata tgcttggaga aaataatcca ataagcaaat tcatacttaa
     3241 caattcagca atattggatt taccagtaag tctttccata ggtttaatag gattatatgc
     3301 ttgtatgtgt attgctttta atctggggaa ctactataaa ttatatatac caggttgtgt
     3361 tgcactatca actttttctt ttttattcct agtagcctca tttacaccag atggagcttt
     3421 agaaatagga aatttaggag ctaaagggct atttacaggt atgatagcag ggcttggttc
     3481 tgtggagtta tataattttt gtaataagaa gaatatcaca ataaaaatgc cagaaaatgt
     3541 acctgatttt gtatctaggt cttttgaatt aatacctact actataatag tttgtggaac
     3601 ttttatagtt gctcgtttta tttcattaaa agtatttgga gaattacctc cacagatact
     3661 tactcaattt ttatctccat tagtaggaag tatggataac ccatggtcag ttcttgcact
     3721 taactttgct atatgtacta tattcttttt tggaatacat tcatcagtat ttagtcctat
     3781 aactagacct ataatgatta catttatagc tgaaaacata gcagctatgc aagctggaga
     3841 aacaatacct catttcttta cagcaggagc ttcaagtgca ttctttggat ttacaggatg
     3901 tggaattagt ataggttgtg taattgcatg tatgttatca aaaaatgctt cctataagaa
     3961 gattggacgt gtatcattat tccctgcatt atttgggata aatgaaccaa tattatttgg
     4021 agcaccgata atattaaatc caataatgtt tataccacat gtatttggtg gagctataat
     4081 aggtacacta ccaatgtttt ttatgcactg gggatggtta gcaaagccag tatttgaccc
     4141 tccatatgta ggggtattct tagaaggatt tttaactaat ggagattggc attctatatt
     4201 agcaaatgca ttgcaattag tattagcagt tttattatat tggccattct ttaaaataat
     4261 ggaacgtcaa caaaataata aaaaacaaaa agaagaaact aagaatatat tctctaaaga
     4321 agaagaagac ttattggatg atttaggatt ggatttctaa tattttgtta tgaataatac
     4381 aatagagaga ttagtatcct taatctataa aagaggtgtt gacgctcttt tgattaaagc
     4441 aaaaaatact aaaagatata taggagcttt atcaggtagt ggtgtatatg tgcttgttac
     4501 taaggataaa aattatcaaa ttctagatgg cagatatatt gatgaagcag ataaaaaaac
     4561 gactggtttc ataaaaagga ttgtatctca aggttcttat ataccaacta taattgagtt
     4621 attaaaagaa ttaaatataa aaaagttagc aatagaaaac caagctatga gtatacaaga
     4681 gtatctttca ctaaaaaatg aaggttttga aatcaattta ataacaaatg aattatggaa
     4741 gctaagggct ataaaatcta aagaagaaat tgaacttatt aaaaaggctt gtgagattac
     4801 agatgaagta tttgatgagg ttatttctga aattaaaaaa gatatgactg agctagaagt
     4861 atcagctcta attcagtatc atgcattaaa aaaaggtgca agtggaatgt cttttgaaac
     4921 aatagtggta tcaggagaaa gaggtgcaat gccacatgga agacctacaa acaaaaaatt
     4981 gaaagtgaat gaagctataa caatagattt tggtgttgtt tatcaagggt atcaatcaga
     5041 catgactcgt acaatatcta taggtaaacc tcctaaaatt attaaagaga tatatgatgt
     5101 agtattggaa gcacaattat ctgcaataga gtcaataaag gaaggaacta gggcaagtga
     5161 tgttgacaaa gtggctagaa aaattataga taaacatgga tttggcgaat attttaatca
     5221 tggactgggt catggtatag gtcttggaga tggtgaagtt cccactttaa atccaaatag
     5281 tgaagatata ttagtggaag gaatggtaat gtcttgtgag ccaggtattt atattccaaa
     5341 tgtaggggga gttagaatag aagatgatat tgttataata gatggaaaag gtgttccact
     5401 caataagaca tccaaagagt ttataatatt aggagagtaa aattatgaaa tataatttta
     5461 ataaagtatt aaatcgtaaa gaaggaaatt gtagaaaatg gagcaaccat gtgattaaag
     5521 aaaaattcgg cttaaatgaa gatgcaattc ctatggattt agctgatatc gattttgagt
     5581 gtgctcctgc tataaaagaa tctatcttaa aaagagcatc agtaggagat tatagttata
     5641 cttttatagg agatgacttc tatgatgcta ttataaattg gaataaaaga agatttgatg
     5701 tatatataga aaaggattgg ataaaattaa cttttggtac tgtttcaacc cttcattata
     5761 tagttcaagc atatactaaa gaaggagagg gtgtattgat aaatacacct gcttatgacc
     5821 cttttgcaga ggctgtagca aataataata gaaagttgtg ttgtagctca ttaaaattag
     5881 ataacaatag atattattta gatttagagg atatggaaaa tcaaatgaaa tacgagaata
     5941 taagattact tatattctgt agtccacaaa atccatcagg tcgtatatgg acaaaagaag
     6001 aactatatca agtgtcagag ttatgtttaa agtataatgt aattttggta agtgatgaaa
     6061 ttcatagaga tataatattt aaagattata gatttgtatc tttgtggaat gctcatccag
     6121 aaatttataa aaatagtata atctgcgtat ctcctaataa aggcttcaac ttaggtggat
     6181 taaaaacatc atatgtatta ataagagatg taaaaattag ggagacttta ttagaaaggc
     6241 taaaaagtaa ttcaataact tctccaaatg tatttgcaat accagcaata gttgcagcat
     6301 acaatgaaag cgaagaatgg ctagatgcta tgactagtta tgtagaggag aattttgaaa
     6361 tagtttatga tttctttgaa actaatatac caaaagccaa agtgatgaag tcagactctt
     6421 cttttttagc ctggatagat gtaagagaag tatttagaaa tgaagaagaa tctaaagaat
     6481 tttttagaca tgcaaactta actatggtag ttggaagtta ttttgtgaaa gatggagatg
     6541 gatttattcg tattaatgtg gggtgtccta gagaaacatt aaatgaggct ctaaatagaa
     6601 tcaaaaaaac gtatataagt atgtattgtt aaatcataaa aaaagtatct atggtttata
     6661 taaaaacaca ttatttagtt atcaaatatt actggaattt tgtattccag taatattttt
     6721 tgattaagga atattattga ggaatattaa ataatattat ttcataccct tatattaact
     6781 tatataaaaa tattttatta ttttgggaac gggttagcta acctttatct agtgtatatt
     6841 tatattgtaa gaagcagtaa aaacttttcc cttactaagt ttattaataa acttcttatc
     6901 tttataaatt ttgttagaat ttatactatc agcaagacca taccaaggtt ctatacataa
     6961 aaaaggagca gtagaattag tttcactata ataaggagtc caaataccaa ctagagggaa
     7021 atcttccata gatatagtta catatttact accatcatta ttatatatag acacttcatc
     7081 tatgttggtg tatatcaaag catcattctt gaatacttct ggatttaaat gtaagttttt
     7141 taattttcca ataggtatta tatcatctgt aaaagaacca tttaaattga ttttttctac
     7201 atcattttta cttttaaact caaggtagtg tttagaaaaa tcattttgtt tgtaaaaagg
     7261 aaggttgaat gcaggatgtc cacctataga aaagaatata tctttagagt cagtattttt
     7321 tacaatccat tctacctcaa tactattatc atttaatgta tagtttatga ttagctcaaa
     7381 agaatatgga tacttcttta aagtggattc attatctgtt aatttataag atacaaatgt
     7441 atctccttta tctacaatct caaagtccat atctctagca aacccatgtt gagtcatgct
     7501 atataaattt tcttctatta ttgtttcatt atcaaaaagt ttacctacta tagggaacaa
     7561 tataggtgat tgtctacccc aatatttttt attaccatcc caaagaatgt ttttgtcgta
     7621 ttttttagaa aatattttag ttagttcagc accaaaactg cttgattcga taattaaatt
     7681 ttcattttgt aatatattca tttaaaatct cctttattat attttatgag tatattatac
     7741 tataaagtat aaagattatt aaattttatt tgatattata tttgaaaggt agattttagt
     7801 atattttgaa aattttgttt tgaaaatttg ggattattta agaaattttg tattataaat
     7861 agaaggaaat agataaattg tatagaataa aatatattaa gataagttaa taatgttaaa
     7921 tgggaagtga gtttatggaa aaaccaaata caaaagttgt taactttcaa gagtataaag
     7981 acagaaaaaa ccaaagagaa gtaaatagag acgaactttt aaagcttata aaaaaaattg
     8041 tgacttcaaa atagggagtt gttaaactcc ctattttaat gggtttcata ataattaaga
     8101 cattctgagt ccatctatat aatctttgaa ttgtgagaaa tctgattcac tacttaattc
     8161 tatggtttct gaaccttccg tataaaaatt cttaagatag atagtataat tattatcaac
     8221 acaatcatga taaatacatc tatatccatc ttcatacaaa ctttttaact tagaaaaatc
     8281 atccacaata tcatctcctt aaagttcttt taaatatatt tttacaaaaa gcatctaatt
     8341 gtatgcaaat tgacaatgta aaataaagga aatgaacata aaatgtagaa taatttttat
     8401 atgtatgtaa aaataaaaag aaaaagttga aatttttaga aaataagagt attttttatc
     8461 taaaaagaga gtttaaaaaa taaaaaaatg ttgggaggat aaaaaatgag tttattagaa
     8521 agtatatcaa aaaacatata ttccctagat aactcttcta ttgaaaaaac aaagcagaga
     8581 ttggataggc ttatacatcc aactggaagc ttaggaaaga tagaagatat ttgcatgcaa
     8641 ttggcaggaa tatttggcaa tgaaaatttt gacacaagta aaaaggttat aattgctttt
     8701 gcgggagacc atggtgtata tgaagaaggc gtagcacctg accctcaaaa tataacaaaa
     8761 ttacagtttc caaatttttc gaaaggtcta tgtggagttg gagtaataag taaatttgta
     8821 ggggcagatg ttgtagctgt agatgtagga ataaactgtg atgaaaaact ggatggggta
     8881 ttagactaca agataagaaa agggacatca aatatggcaa aaggtccagc tatgagtaaa
     8941 caagaagcca ttagatgttt agagataggt attgaaatag cagagcagtg tatagaaaaa
     9001 gattataagg ttattggtat tggggagatg ggtatagcaa atactactcc aagtacagca
     9061 ataatatctg taatttcagg gtgtgacccg ttagaagtta ctggtatagg tgcaggtctt
     9121 aagaaagaaa gactaaagca taaggctgaa gtaattagaa aggctattga aattaataat
     9181 ccaaatccaa ctgatggagt agatatttta tctaaagtag gaggatttga aataggctca
     9241 atggcaggtg ttatattagg ttgtagtgct aataggattc cagtagtaat tgatgggttt
     9301 atttcttatg cagcagcatt aattgcatat aagataaatc caaaaactag agaatatatg
     9361 atagcatctc atttatcagc agaatcagga actaaaagag cattagacat attaaaatta
     9421 gaccctctat taaatatgga tatgagatta ggtgagggaa gtggagctgc tttagcattt
     9481 gatataatag aagcttcaaa ctatacttac aaaaatatgg caacttttga tgaaattgac
     9541 atgggaagat aaatataaaa atttaatatg taggagtgtt ttgatgagta aaattattct
     9601 agtaacagga ggagctaggt cagggaagag taactttgca gaaaaactat gttttgacag
     9661 aaataataat acagcttata tagcaacctc tattccattt gatgatgaaa tgaaagatag
     9721 agtaaaaaaa catcaagaga atagacctag aaactggaat acttatgaaa tatatgaaga
     9781 tatatattcc ataattaagg agatatataa agaacatgaa actgttatac tagattgtgt
     9841 aacattatta gtaaataatc taatgtttga acacaatcta aatatagaag agtctagtca
     9901 agaagaaata aacaaattag aacaatacat aaaagaacaa gttgtcaaac ttattgaaga
     9961 aatagaaaaa acaaatctat actttgtatt tgtaacgaat gagttaggta tgggaattgt
    10021 tcctgggaat aaacttagta gaatttatgc tgatattgta ggtcgtataa atcaatatat
    10081 agcttcgaaa agtaatgaag tttattttgt agttagtggc atagctatga agataaagga
    10141 gtagtatgca attatgaaaa gatttatttt aatactacag tttttgacaa ggatacctat
    10201 aaaattaaat gtaggatttg atgatgaatt ttataagtca atagtatatt ttccacttgt
    10261 aggttttgtc ataggtatat tatcctattt aataggatgg atttctatgc ttttatttga
    10321 accatttata gcttctataa taataactct agctggtgtc ttaattactg gaggacttca
    10381 tatagatgga ttaggagata catttgatgc aatatatagt tatagggata aagaaaaaat
    10441 gcttgaaata atgaaagatt caagattagg tacaaattca cttctagcaa ttatgtttgt
    10501 acttttatta aaggttggat tcgtttacga tataataagt aataattcat tgtggattat
    10561 aatttttatg ccaatgattg caaggttagg agtaatgtta ttgacatata aaaccgtgac
    10621 accaagagaa aaaggaatgg gaaatttatt tataggaaaa ttaactacaa gtatgttaat
    10681 aactgctata atatacacat tattgatagt tgctcttatt actaaattta tatttttatt
    10741 acctaatata gtgttgatta aagtattagg ttcgattata gtggtttttg tatttataat
    10801 attattcaaa aaacatattt ataaaaaaat tgacggagta acaggagata tattaggctg
    10861 tggaatagaa ctttcagagc ttgtatattt aatttacata tatttgttaa tttttatgtt
    10921 tttttaaagt aaaaagtggt gataatatga taagattaat attaataaga catgcactga
    10981 caaatgataa taaaaaagga agattatcag gccatataaa tagttgtatt agtgaagagg
    11041 gcaaactaca aataaataaa attaccagat atctgagtaa tgaaaacata gataaggttt
    11101 atacaacacc atctacacgt actaaagata cagtagaaaa aatatcaaaa ttgaaattat
    11161 tagaaataga agaaaaagaa gctttaagag aaattagttt tggagacttt gaaggaagaa
    11221 cttttgaaga aattaaaatc aaatatccaa atgagtttga aaaaatgata aaagaaggta
    11281 acaactatag atatccaaat ggagaaagtt tgatagactc ttataaaaga gtggcaaaag
    11341 aaattgataa tataatttta gaaaatgata gtaatttaga tacaaagaca atacttatat
    11401 gttctcatgc aggaacaata agaaatataa taacacattt gatttctggt agttataagt
    11461 atcactggaa ctttaaaatt gataatgcat ctattacagt tttagaaatt gatggtggat
    11521 ttgctgtaat agataaaatg aattttacag acttcatata atttaatcaa aaccaaacat
    11581 tacaatctgt gtttgtaata tatctgtaaa attagcaatg aaaatagaat attagattaa
    11641 aattaggttc ttaattagat gttaaaaggg aaaaaggtta gatgcctttg cagcccccgc
    11701 tactgtgaaa ccaacgaaac tataaatacc actgtcagtt tgatgggaag gttatagaag
    11761 taggatgaag ttaagccagg atacctgcct aatttaattt aaaacaagag gtcttcgggg
    11821 tgaaagattt ttaattaata tatataaaag tcctatcttt agaagtatta ggactttttt
    11881 taatttgttt tttagtaaat tttaacaagg agggatggaa agtggggaaa aaaataatgc
    11941 tacaaggaac agcttcaaat gtaggtaaaa gtgtcttagt agcaggtttg tgtagaatat
    12001 ttaagcaaga tggttatact gttgctcctt ttaagtcaca aaatatggct ttaaattctt
    12061 ttataactaa agaaggatta gaaatgggaa gagcacaagt atttcaagca gaggctgcga
    12121 aaatagagcc aatagctgat atgaatccag ttttattaaa accttctggg aataataaaa
    12181 gtcaagttat tgtaagaggt aaagttgtag gagaaatgcc ttcttctaag tatcatgatt
    12241 ataaattgga gttagtaggt gtgttgaagg aaacatttaa tagcttaaat tcaaggtatg
    12301 atattgtagt tatggaaggg gcaggaagta atgcagagat aaacttaaaa gatagagata
    12361 tctctaatat gggtatggca gaaatagctg atgcacctgt aataatagtc ggagatattg
    12421 atagaggagg cgtatttgcc tcattagcag gaacaatgct tttattaaat gaagatgaga
    12481 aaagaagagt aaaaggtgta ataatcaata aattcagagg aaaaaaagag cttttacaag
    12541 atggtgtaaa gatgctagaa gacatcataa aagtacctgt tataggagta gtaccttata
    12601 cagatataaa aatagaagat gaagacagtg taactacgag atttaagaaa aaaatgaata
    12661 aaagtgatat acacatagaa ataattagaa ccccacatat gtcaaatttt acagatttta
    12721 atatatttga gacacaagaa gatgtaagta ttagatatgt agactatgga gaatctttag
    12781 gaaatccaga tatattaata atacctggaa ctaaaagtac tatagacgat ttaacatata
    12841 taagaaaaag tggtcttgaa tcgcaaatca aaaatttaca tagtcaaggt aaattgatat
    12901 ttgggatatg tggtggatac caaatgttag gtaaaaaatt aaaagaccct tatcatgttg
    12961 aaagtgaaat tgaagaagtt gatggaatag gtcttttaga tacagaaaca acgtttgaga
    13021 aagaaaagac tacaacacaa gtagaggcta ctattgttga atgtatcagt gaatacatga
    13081 ataaccttaa gggaaaaaaa gtaaaaggtt atgaaattca tatgggcatt acaaagcgag
    13141 gtaatgatgt aagaagttta gatttaataa ctaaaaaact tgataaagaa gtgaattata
    13201 ctgaaggaag tgtaaataaa gaggggaatg taattgggac ttatcttcat ggaatatttg
    13261 atgaaataag ctttacaaga gcaattttaa acaatataag aaagaaaaaa ggtttaggag
    13321 aattagaaag caaagtaaac tcttttaatg aatttaagga taaagagtat gataagttgg
    13381 ctgatttttt aagagaacat ttagatatag agaaaatata tgagattatg aaataatgat
    13441 gacctttatt tataatttag gtaggaggtg tattatgaaa aaaattttaa ttgctggtac
    13501 taatagtgga gtaggtaaaa caacaatatc attaggaata atgcaagctt tggttaagag
    13561 aaatatgaaa gtacagccat ataaagtagg gccagactat atagaccctt catatcacac
    13621 ttttataact ggaagacatt ctagaaattt agattcttat atgttagatg atgaaaaaat
    13681 aaagtatata gtaaataagt cctcaaaaga tgcagatatt tcagtaatag aaggtgttat
    13741 gggcttatat gatggttttg gaatagattt agataattgt acaagttcac atacttcaaa
    13801 ggtattaaag tcacctgtta tattagttat aaatggtaaa tctatggcag catctagtgc
    13861 tgctatggta cttggatata aggaattaga taaagatgta aatataaagg gtgttatagt
    13921 aaataatgta aagactaata gccattatca aataattaag agttctatag aaaaatattg
    13981 taatgttgaa gtattggggt attttcctcc aaataacaaa ttctctttag aatctcgaca
    14041 tctaggatta gttcctagtg tagaaatgaa atcactaagg gaaaaatttt ataccttagc
    14101 agatgaaatt gaacactata tagatattga cagaataatt gaaatatcag aaagcgatga
    14161 atttgagagt gattttgatt ttcaaacttt gatagaaaat aataaaataa aagaacatgt
    14221 taataataaa tcaatagcta tagcatatga taaagcattc aatttttatt atagagaaaa
    14281 tatagagctt ttagaggaac ttggtttaga aataaactat tttagccctc ttgaagatgc
    14341 atcagtgcca aatgcagatt atatttatat tggaggaggt tttccagaaa tatttggaaa
    14401 agaattagaa gcaaatgagt caatgagaac ttccatatta gaagcacata acaataatat
    14461 acctatttat gcagaatgtg gaggactgat gtacctaggt gagaaactat tgaatcaaga
    14521 agaacaagaa tttaatatgg ttggaatatt tagtggaatt agtaagatga catcttcttt
    14581 aaagagattt gggtactgta ttggtgtagc caaggaaaat actcttttat ctaaaaaagg
    14641 ggaatctatt aaagggcatg agtttcatca ttcaatattt gaaagtgatg aaaaatgtgt
    14701 atattcaatg agaaaagaaa gagatggaaa tatagtagaa gagtgggatg gtggttatag
    14761 taaaggaaac actttggcta catatcttca tactcatttt tataataact taaattgtat
    14821 agaaaatttt ttgaaaaaag gaaatgaata aatgagtatt ttatctattt atataggata
    14881 tgtgacagac ttaattgtag gagacccata ttcttttcct catccagtga gatttattgg
    14941 taaacttata aattttactc aaggtataat aagaaaggtc tctaaaaatg ataggcagtt
    15001 aaaatttggg ggatttatat tatggtttat aaccgttgga ataacatatt taacaactta
    15061 tgctatagtg aagctattta gttttaatat attattgagt attgtagtga atagttttat
    15121 aatttataca acactagcca caaaatgttt aaaagacgaa gcattaaaaa tttataatgt
    15181 attaaaaacc gaagatatag aaaaatctag gattcagcta tcttatatag taggtaggga
    15241 tactactaat ttaagtgaac ctgaaataat aagagcaact gtagaaactg ttgcagaaaa
    15301 tactgtagat ggtataattg cgcctatgtt ttatgcattt attggaggag caccacttgc
    15361 tatggcctat aaagctataa atacattaga ctcaacagtt ggatataaaa ataaaaagta
    15421 taaagatatt ggttttgcat ctgctaagat tgatgatata gccaattata taccagctag
    15481 aatatctgtt attcttatga caattggaag tttcttttta aattataact ataggaactg
    15541 ttttaaaatc tctattagag atagaaagaa tcataaaagt ccaaattgtg ctttttcaga
    15601 aggcgcagta tctggtgctc taggaataca actaggaggt accaacgttt attttgggga
    15661 aaaagtatat aagccaacaa taggggataa gcttagggag atagataaag aagatataat
    15721 taaaactaat aaaattatgt atgtatcttc atttgtatct atgttggtat ttacactaat
    15781 acatgtgtta tgtaggatga ttttaattaa cttaattata taaattattg tttgctaact
    15841 taagggggac atagatgaag gatttagggc atggtgccaa tgtaggtgaa atggcaagat
    15901 tatatgggaa aaacccaaaa gaaataattg attttagttc aaacataaat ccaaatgtat
    15961 taccaaattt agagagatac attttaaaag gattagaaga gtgtaggaat tatccagata
    16021 taaattatac aaacttgaga gaaaatatat caaaatatat aggtataaat ccagacttta
    16081 taatacctgg aaatggtgca acagaagtga tttatttgct tatgaagagt gttaaaaaaa
    16141 aattggctat aataaatccg actttttcag agtatagaag aagtgctgag ttaaataatt
    16201 tagatattat agatttagag ctagatttag aaaataactt taaacttaat atagatataa
    16261 taaaagaaaa tataaaaaga tttgatagtt tatttatatg taatcctaat aatcctagtg
    16321 gtaatgtcca agatttaaag gaacttgttc atttactagg taaacataat aaggtactaa
    16381 taattgatga aactttcatg gaatttgttg aagatgaaag taaatacagt ttggttaaat
    16441 atatagaatc aaataaaaat atatttataa ttaaagctgt gactaagttt tttggtatgc
    16501 ctggtttaag gctaggatat ggtcttacaa gcaacactga aattatgaac aaaatttatg
    16561 aacacaaaga gccatggact attaattctt ttgcagatat actatccaat tttatttttg
    16621 aagataaaga gtatattaaa aatagtaaag aatattacat agaagaaaga aaatatatgt
    16681 tacaagagtt gagaaacata agaaatataa aggtctatga tacagatgct aattttattt
    16741 tgataagagc atataaaaaa acaactaaag aattgaaaaa agatttgctt aagcaaggaa
    16801 acatattagt tagagatgcg tctaatttta taggattaga tgatagtttt ataagagtgg
    16861 ctataaaatc tcatgaagat aacaaaattc ttatagaaaa tatgaaaaat ttattgggtg
    16921 attaaaatga aatcttatgg aatatgccca gcatcttgtg gagaatttgt acaaggtata
    16981 attgatgatg aagaatattt atgttcatat gcaatagata tgtattctga agtgtatatt
    17041 gaagaaaaat tagtagatat aaatttaggg agatataagt caaggttagc aattgaaaag
    17101 gtatttgaaa aatttaattt acctaaaaaa tatactaaaa atatttcttt aaatataaat
    17161 agtaaaatac ctgttggaaa aggaatggca agttcaactg ctgatatagg agctacaata
    17221 aaggcaacat tatcattgat agataaagat ttaagtagtg aggagatttc taaattagca
    17281 gcagaaatag aaccaacaga ttctatattt atagataaaa atagtatatt taatcctcta
    17341 aatggaactg ttataaaata cctaggaaat ctgaccaatg cgaaagttat aatacttgaa
    17401 ccaaacaaag tactagatac tatgaaaatt agattgaggc aagattataa taaattaaaa
    17461 gtagaaaata aagaagtaat aaaaaaatca tttgctcttt tagaagaagg attgaaaaaa
    17521 aataatttat ctctagttgg agaggcttgt actttgagta gcttagcaaa tgagaatata
    17581 gaaaaaaaag agtatttaaa tgaaataata aaaatatcaa aaaaatatgg agcatatgga
    17641 gtaaatattg ctcacagtgg aactgtagta ggtattttaa ttgataagtc tatgaatgat
    17701 aaaaagatga tagatacctt atgtgaatct aacataaatt ctgtctataa taaaatttat
    17761 acacaaaaca taattaatgg aggaattaag ggggaaatag aatggaatac ataaagaatc
    17821 ctatgaaaat agaagaaaaa agttttgaat tgatacaaga aataatagac gaaattagac
    17881 cagattataa atttaagaat aatattgaag aaaaaataat aaaaagagct atacatacta
    17941 ctgctgattt tgattactta gatatattaa aaatatcaga agaagcagtt gatagtataa
    18001 tagatgcatt aaaaaataat gcaagtattt atactgatac aaatatggct cttagtggta
    18061 taaataaaag aaagttagaa tcattaggat gtaagtataa atgtttagtg gcagatgatg
    18121 aaactgtaaa attagctaaa gaaaaaggaa ttactcgttc tatggcagca gtagaaattg
    18181 cagctaaaga agaaggaaga aaaatattcg ttttaggtaa tgctccaaca gctttatata
    18241 aagttatgga gatgaaatca gaaggtagat tagatttaga tgcagttata ggagttcctg
    18301 taggttttgt aggagcacaa gaatcaaaag atgaagttga aaaaacagat ataccatata
    18361 taatttctaa aggtagaaaa ggtggcagta atttagcagc agcaatagta aatgctatat
    18421 tatatactat gtaggtacct atatggaaga atatgtatat atagatggaa aaaaatatag
    18481 aagaggatat actacaggtt catgtgcaac aggtgcatct aaggctgctg tatatatgtt
    18541 gattacaaaa aatagaatca atactataaa tatagatact cctaagggga taccattact
    18601 actaaaagtt gataatataa atatttctga tacatttgtg gaatgttcca taaaaaaaga
    18661 tggtggagat gatatagatg caactcatac catggatatt tatgcaagag ctgaaattgt
    18721 agctaaaaat gataagaaca aagggtatct tactctaaag gatatagata gtttaagcac
    18781 caatagtgag tgtaaaagtg agctctataa gtttattagg gtttatggcg gaactggtat
    18841 tggtgtggtt acgaaaaaag ggcttagtgt ggatgttggt aaacctgcta taaatccgac
    18901 tccacttaaa atgataaacc atgaaataag aaagcttata ggagataatt ttgaatctat
    18961 acttggaaat gataaagttt taaaaataac aatctttgct ccacaaggag aaactgtagc
    19021 aaaaaaaaca tttaatccaa gattaggtat agttggagga atatctataa taggtacaac
    19081 tgggattgtt gaacctatga gtgatgaggg ttggaagaag tctctttcta tagaacttca
    19141 gatgaaaaaa gaacaaggct tagataaaat aatattggtt ccaggaaatc atggagaaca
    19201 gtttataaga gaaaaattaa acttagatat taagtatgta gttagggtaa gtaattttat
    19261 aggctatatg attaaagaag ctcaaagaat aggatataaa aaaatcttaa tggcaggtca
    19321 tataggtaaa tttataaaag tatctgctgg tatttttaat actcatagta aggtagcaga
    19381 tgctagaagt gagatattag tggcaaactt agctttaatg ggagctagat acgaattttt
    19441 aaataaaata aatcaatgcg taactactga agaagctgta gagctaataa ataatagtga
    19501 atatagagaa gtatataata tattaagtaa taaatgtaag gagagagtaa agcaatactt
    19561 gaatgaagat tcagatgata ttgatgtgga agtaatcata ttttcaatgg ataagtcttt
    19621 attaggtaaa tcggataata cagatgattt agtggaggtc tttatatgat aaacataata
    19681 ggtctaggac ctggtaactt agactatata acaaaaaaag gtgaaaattt aatttccact
    19741 tctgatgtat taattggtgg taaaagaaat ctagagtcta taaaaaattt tgaaggagaa
    19801 aaaatagttc tggattctaa tttaagagaa attatagagt acataaataa caataaagaa
    19861 aagcaaatat caataatagc atctggggac ccattaatat atgggatagg tagatatcta
    19921 tctaaaaata ttgataataa gatgttaaac gtggtttctg ggattagttc attacagtac
    19981 atattttcaa aaatatatgt agatatgaat gatgtatata ttacaagtag tcatggcaaa
    20041 ataccagatt ttgattacat attatcacat aaaaaagtat gtatggttac agattcaaag
    20101 ataggaccaa aacaaataag tagagaaata atagatagaa atctaaataa aattattgtt
    20161 gtaggagaaa atctgtcata tgacaatgaa aaaataacaa tagcgaaacc agaagagata
    20221 ataagaatgg acaattttga tatgaatgtt gtggtgattt tagatgagga atagtgaatt
    20281 tataacaggt aaagttccaa ttactaagga agaggttaga gcaatatcaa taagtaagtt
    20341 aaatttagta aatgctaaaa acctcataga cataggggct ggtacaggaa gtgtaagtgt
    20401 tgaagctgca tataattatc ctaatttaaa ggttatatct atagagaaaa atgatgacgc
    20461 tatagagctt atagagaaaa atataaaaaa attcaactta aaaaatatag aagtgataaa
    20521 aggatatgct ccaattaatc tagggttaaa tgttaaagtt gagtctattt ttcttggggg
    20581 aacaggaaat aatctggaag aaataataga atggtctaaa aaaaatctcg ttacaggagg
    20641 aagacttgta gctaatttta taataataga tacatttaat caaactttaa aactgcttaa
    20701 aaagcatgga tttaaagaaa tagatgtatg tgttttgaat gtttctaaac tagagaaact
    20761 aggaaaaggt gaatatttta aaccactaaa tcctatatat ataatatctt gtgaaaaagg
    20821 ggaaaatgat gatgaataaa gtacattttg taggagcagg acctggagat aaagagctga
    20881 taacattaaa aggatacaaa ttattaagta atgcagatgt agtgatatat gcaggttcac
    20941 ttgttaatcc tgaacttttg gaatactgta aagaagattg ccaaatacat aatagtgcac
    21001 atatggattt acaagagata atagatgtta tgagagaagg tatagaaaat aataaatctg
    21061 ttgtaagact gcaaacaggt gatttttcta tatatggttc aattagagaa caagtggaag
    21121 atttaaataa attgaatata gattatgact gtactcctgg agtaagttct tttttagggg
    21181 cagcatcatc tttaggggtt gaatatacag ttccagaaat ttctcaaagt gtaattataa
    21241 caagaatgga aggcagaact ccggttccag agaaagaatc aatacaatca tatgctaagc
    21301 atcagacatc tatggtaata tttttatctg ttcaagaaat tgagaaagtt gtgtctaaat
    21361 tactggaagg tggatatcca aaagatactc ctatagcagt tatatacaaa gcaacttggg
    21421 cagatgaaaa aatagttaaa ggaactttaa gtgatatagc tgtaaaagtt aaagaaaaca
    21481 acataaataa gactgcttta ataatggttg ggagattttt aggagaagag tataataatt
    21541 caaagttata tgataaggat ttcaaacatg aatatagagg gtaatattgc tataatctgt
    21601 attacagaaa atggaaaaaa tttagctgta aagataaatt ctctagtaaa taactgtgtt
    21661 atatatcaag taaaaaataa aaaaagtaat ttagttgttg aagaaacatg tgtacataca
    21721 gttcaaaaga agttaagtga ttttgtagga gaaatattta gcaaatttga ttacataata
    21781 tttataatgg ctacaggtat agttgtacga agtatagcac catacataat aagtaagttt
    21841 agtgaccctg caattttggt aatggatgaa aaaggcaaaa acgtaataag tttattgagt
    21901 ggtcatattg gaggggcaaa cagcttgaca cagtacataa gtagtcttat tgatgctaat
    21961 ccagtaataa caacagctac tgatataaat caaaaagctt ccttagatat gatagcaaaa
    22021 aaactaaatg cacatattga taattttaga gaaagtgtaa aagaagtaaa ttctatgcta
    22081 gtgaataata aatgtgttgg attatatgtt gatgaaggat atcaagttga tactagggga
    22141 tttaaactta tagaaagtaa ggatgagtta agagatagct tttcaaacac atatatagaa
    22201 aaattagttg tagtaagcaa taaaataaat tgtattgaag atattgagaa tttaaactta
    22261 gataataaat ttgaacattg ctatagtact aaaaaaataa taaaggtaat tccaagagat
    22321 atagtgatag gaattggctg tagaagaaac acagatagtg agctgatgta taattcactt
    22381 gaagagtttc ttaataaaca aaatatagat attagagcaa taaacacagt tggaagtata
    22441 gaactgaaaa aagacgaaaa agcaataatt gaattatcaa aaatactaaa agtgccattt
    22501 aaaacatttt caatagatga aatatctaag gttgataatc tttttgataa atctgatttt
    22561 gttaaaaaaa atgtgggcgt gtattcagta gcagaaccta tagcatatct tttgagcaaa
    22621 ggtaatttgt tagttgaaaa acataaatat aaaggaataa ctttttcagt ggggagaata
    22681 aaaatatgat atacgttgta ggaattggac caggaagtaa agatacaatg actttagaag
    22741 caataaaagc aatagaagat tctgaggcta ttgttggata taagacttat ataaaactaa
    22801 tagaagaatt tatacaaggt aaagaaataa tccaaaatgg tatgaggcaa gaagttgata
    22861 gatgtaaaca agcagtagag gaagcgaaaa aaggaaaaaa agtagcagtt gtaagcagtg
    22921 gtgatgctgg aatatatggg atggcaggtc tcattttaga attaatatct aaagaagatg
    22981 aagatataaa agtaaaggta attccaggtg ttactgcaag tattggtgca gcagctatac
    23041 ttggagctcc tattatgcat gatttctgtc atataagttt gagtgattta atgactccgt
    23101 gggaagtaat tgaaaaaaga ttgagattgg ctgctgaagc cgattttgta atttgtttgt
    23161 ataatccaag aagtaaggga agaagtgaac atctaagcaa agcatttaaa ataatgggtg
    23221 agtttaaagg tggagataca cctgttggag tggtaaaaga tgtaggtaga gaaaaagaag
    23281 aaaagtttgt atgtactttt gaaaatatgg actttgaaaa agtcgatatg actacaatgg
    23341 ttataatagg aaataagtct acctatatta atggtgaatt gatgataact ccaagaggtt
    23401 atacagtatg attttaattt taggaggaac ttcagatagt ttatctatat gtgataagat
    23461 aaatgaactg aaaaatcaac catatatcct ttcagttact acagattatg gaagggaact
    23521 tgcacaacaa catgcagaga atgtaatttt aggtaagctt ggaaaagaag atatgctaaa
    23581 atttataaaa gataataata tagtaaaaat aatagatgcc actcatcctt atgcagttga
    23641 agtatccaaa actgctatta cttgtgcaaa attattgaat atagattata taaggtttga
    23701 gagaaaatct ttaattgaag aaatagatta tgataataaa tttatagtag atacaattga
    23761 agaagcctgt gaaatagcaa ataaaatagg gacaaatata ttcataggaa caggaagtaa
    23821 aaatttgaac agatttatag aggaaataag tgataaaaat ttagttgtta gagttcttcc
    23881 aacgagtgaa gtcatattaa gttgtgaaaa attaggttta agtgcagata atatagtagc
    23941 tatgaaaggt ccatttagcc agtatataaa tgaagaaaca tataaacatt acaatataga
    24001 tttagtcata acaaaagaaa gtggaatagc tggtgggttc ttagagaagg taaatgcatg
    24061 taaaactttc aatataccag taataattat aaaaagagaa tcgattaact acgaaaatgt
    24121 atttaatgac ataaataaaa ttaaagacat acttgtgtaa tttttaggag gagaatatat
    24181 gaagaaagcc ttattagtag tgagttttgg tacaagctat catgaaacga gagaaaagac
    24241 aatagatata tgtgaagata aaataaaaaa ttcgttaaaa agccatgatt tttttagagc
    24301 ttatacatca aatatgataa taaataaaat aaaaaaaagg gatggaatag aaatagataa
    24361 tccaatacag gctttagata aaatatatga acaaggatat gatgaagtaa taatccaaac
    24421 acttcatatt atatgtggcg aagaatttaa caaattaaaa gaacaagtag atggatattc
    24481 ttctaaattc aaaaagattg tattaggaag acctttgctt acacatattg aagattatca
    24541 ggaagctgta gaggcaatta agcatcaaat tcctcatatg gaaagtaatg aagcagtagt
    24601 atttatggga catggaacac ttcatgaatc acattcagcg tatccagcat tggaatacat
    24661 gcttagggat aatggaataa atgcttatgt tggtacagtt gaagggtatc cagaaataga
    24721 acatgtaata agaagattaa aagaaggaaa tataaaaact gtaaatttga tgccatttat
    24781 gttagttgca ggagaccatg ctataaatga tatggcagga gatgaagaag attcttggaa
    24841 aacaatatta gaagaagatg gatttgacgt aagagttcat ttaaaaggac taggagaaaa
    24901 ttcttatata caagataaat ttgttcgtca tgctgtaaaa tgtgcagaga atgctaagtt
    24961 ctatggtata ggagcagggc caggagatgg ttctttactt acgataaaag cggttaatac
    25021 tttaaagaaa ctggatattc tatacactcc agaatcaaaa aagggtggag atagtctagc
    25081 tttatcaata gttagtgaat atttacctga atcagtggaa ataaagtcta ggcatttccc
    25141 aatgagtttt gatgggaatg aaaaaagttt agcttgggac aatgtagcac aagaaattat
    25201 atcagatgtt aatgatggta aaaatgtagg atttgttaca ttaggagacc ctatgattta
    25261 tagtacatat gtatatataa tgaaaagaat attaggagag gtagaagtcg aaactatacc
    25321 aggaatttca tcattttcca atatagcatc taatcagaat tttccattag tcatggataa
    25381 agaagcttta gtggtaattc cttgtactat ggaagatgat aagatagagt atgctttgca
    25441 aaattataat tcaatagtat tgatgaaggt atataaaaat tttaaagaaa taataaaaaa
    25501 attagaaaag tatgatttaa tagaacatgc tattttagta agtaattctt cccaagaaag
    25561 cgaaaatgtg tttacagatt taaaagaagc gcaccttgaa gataagatat cttatttttc
    25621 tactatacta gtaaataaag ataataaaat aaaataggtg aaagcaatat ttaaaggaga
    25681 cttaatatga atatagtggt tggaactagg ggtagtactc tagcgcttat acaaacagag
    25741 tgggtaataa atgaattaaa aaagaagtat ccagaaatta gttttgagat taaaatcata
    25801 aagacaaaag gagatttaat tcaaaatgtt tcactggata aaataggtga taaaggattg
    25861 tttgtcaaag aaatagagca acaattatta gatggaaaaa tagacatagc agtacatagt
    25921 atgaaagata tgccttctta tttagcagat ggactaaaat ttgctcatac gcctaagaga
    25981 gaagacccaa gagatgttct aatacttaga gaaggatata aaaatttaga tgatttgcct
    26041 catggagcag taataggaac aggaagtaaa agaaggaagt ttcaattatt gaaacaaaga
    26101 cctgatttaa atatagtaca agttagaggt aatgtggaaa ctaggatacg aaaaataaaa
    26161 gatgaaaaca tgcatggtat tgtgttggca gcatcaggaa taattagagc taatttgcaa
    26221 gataaaataa gttcttattt acctgttgat gttgttattc cagcacctgc ccaaggagct
    26281 ttagctatag aaattagaag caatgatagt acgatagagg gaatagtaaa ttcactgaag
    26341 gatgagaata cagaaatcca aatattggct gaaagaggtt ttttagatgg tgtaaatgga
    26401 agttgtcata ttccaatggc agcttattgt gaaataatcc aagataaaat acatcttact
    26461 ggcttatatg gagattcaga aggaaaaaaa gtagtaatca agtctataga tggagatatt
    26521 tcttctccta gagagttagg tttaaagtta gctaaacttg tattgaagga gtatgagaat
    26581 tatgaagggt aaggtttact tagtaggggc aggaccaggt gattataaac tcatgacttt
    26641 aaaaggttta gagtgtataa gaaaatcgga tgtaatagta tatgatagat tagcaaatag
    26701 tagttattta agagaagcta agcctgattg tgaacttata tatgtaggca aggcatcaag
    26761 taatcatatt ttgccacaag aagatataaa tagaattata gcagaaaaag ctaaagaagg
    26821 aaaaattgta actcgtctta aaggaggaga cccttatgta tttggcagag ggggagaaga
    26881 agcagagact ttagtagatg aagatataga atttgaagta gttccaggta taacttctgc
    26941 tataggaggt ttatgttatg cgggtattcc tataacacat agagaccatg cttcgtcatt
    27001 tcatgtaata acaggtcatt tgaagggtga tgatagtgga gaattaaact ggaatgctct
    27061 tgcaaataac aaaggtactc ttgtgttttt gatgggaatt tcaaacctag agaaaataag
    27121 tgaaaactta atgaaagaag gaaaagataa ggatacacct gttgctctta taagttgggc
    27181 aactagatat aatcaaagag ttgttacatc tacacttgag aatgtttatg aaactgctat
    27241 taaagaagaa gtaaaaccgc caactcttat agtagttggg agtgttgtag gacttagaga
    27301 aaagttgaac ttctttgaaa gtaagccttt atttggaaaa agtattgctg taactaggtc
    27361 tagaaatcaa aatagtgtat tggtagaaaa aataatggat ttaggtggaa atcctataga
    27421 aatacctact attaaggtag aaaaaataca aaataatatt aacttagaaa atgaaataaa
    27481 aaatataaat aagtataatt atttaatact aactagtaaa aatgccgtag aaatcttctt
    27541 tgaaaagata tatgaaatga attttgattt aaggatatta tctaatttaa aggtatgtgc
    27601 tataggttct gctacatcaa atgagttgaa aaaaagagga attatagcgg atatagtacc
    27661 aaaaaaattt gtagcagagt cactgtatga agagttagct cctattttaa atgataacga
    27721 taatatactt atacctagag cagaaaatgc tagagattat ctggttgaca aacttaaggc
    27781 tatttgtgaa gtaacagaag tacatatata tagaacagta atagatgagg ataaaaaagg
    27841 tgagatatta gatattttaa attctaatga tatagattat ataacattta ctagttcctc
    27901 aacagtaaat aatcttgttg aaattatagg aaaagaaaat attaaaatat tagaaaaaat
    27961 aaaaacacta tctataggtc ctataactac agagacaata aatagtttgg gaataaaatt
    28021 gtacaaagaa tctgctacat ctactataga ttcgttagta gatttaatta taaaaaatta
    28081 attttggagg cttgaatatg ttaagaagac caagaagact aagagcaagt aaagcaatca
    28141 gaaatttggt aagagaaact aaggtgaatg tagaagattt aatatatcca ctgtttgtag
    28201 tagaggggga aaatataaaa gaagaaatca attctttacc agatgtatat catttatcaa
    28261 tagacatgct agaagacgaa ataaaagaaa taagtgagtt gggaattgaa catataatgt
    28321 tatttggtgt tccagatgaa aaagatgagt gtgcttcaca agcttttaat gataatggaa
    28381 tagttcaaaa ggcaattaga aaggtaaaag aaataaattc agatataaat gtaataacag
    28441 atgtctgtat gtgccaatat acaagtcatg gacattgtgg gattttaact gatactggtt
    28501 atgttgataa tgataaatca ttggagtatt tggctaaaat atctgtaagc catgctaaag
    28561 caggagctga tatggttgct ccatctgata tgatggatgg aagaatacaa gcaataagag
    28621 aagctttaga tgaaaatgga ttagaaaatg ttggaatcat ggcttatagt gttaagtatg
    28681 cttcaaatta ttatggacct tttagagagg cagctcattc agcaccatct tttggagata
    28741 gaaaaacata tcaaatggac ccagcaaata gcaatgaagc tataatagag actgaacttg
    28801 atatattgga aggtgcagat atacttatgg ttaaacctgc attatcatat ttagatatta
    28861 taagaagagt taaagataat tttgacttac ctctagcagc ttataatgta agtggagaat
    28921 actctatgct taagtttgct gtgaaggaag gtttattaaa tgaagatgta attcttgaat
    28981 caattacatc tataaaaaga gcaggagcag atataataat aacttatttt gctaaagaca
    29041 ttgctaaact gttgaaaaaa taaattctag tagtataaag aatatcaaaa agtataggaa
    29101 ttgtaattaa ctattataca gtagttttgt ttagacattt attgaagaat aatagaaact
    29161 aatttacaat ttctatactt tatttatgta actaaataaa atttggaggt agaagatgct
    29221 atatcctatt aatttaaaat tagatgagtt agatgtagtt ataattggtg gtggagaagt
    29281 tgcatatagg aaatgtaaaa attttttaga gtttaacaaa aatgttacta tagtttctaa
    29341 acaaatttta aataaatttt atgatttaaa aggaaatata aaaataataa aagatgatta
    29401 taaagaaata tatataaaag atgcttctgt aataattgct gccacaaata atagagagtt
    29461 aaatatggaa ataggattat attgtagagc aaaaaataag ctagtgaatg tagttgataa
    29521 tatagaaata tcgaacttta ctgttccatc atatataaaa agaggagatt tacttataag
    29581 tgtgtctact gggggcaaga gtccttcatt atcatcaaag ataaaaaaag aaattgaaga
    29641 gagatatact gaagattatg aggagtattt aaatgtatta ggagatataa gaaaaaaggt
    29701 tataaaaaaa tacgaagata aaagtaaaag aaaagatgtg cttaatatgt tggtaacact
    29761 tgacctagaa gaacttaaga agtttgatat ataaaaaata aaataaaaaa tgttaaaaag
    29821 gtgttgacgg attattttta agatgatata gtgttacttg tccttaagaa aagggcaaac
    29881 ataaaaaa
//
LOCUS       DAELXX010000040        29581 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.040, whole genome shotgun sequence.
ACCESSION   DAELXX010000040 DAELXX010000000
VERSION     DAELXX010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29581
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.040"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(78..2048)
                     /gene="ftsH"
                     /locus_tag="KPH29_001722"
     CDS             complement(78..2048)
                     /gene="ftsH"
                     /locus_tag="KPH29_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBG1467322.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIVVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(2082..3470)
                     /gene="tilS"
                     /locus_tag="KPH29_001723"
     CDS             complement(2082..3470)
                     /gene="tilS"
                     /locus_tag="KPH29_001723"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBG1467323.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDSEGIICVGDYKISENYKIDEN
                     TKEVLKINFNKL"
     gene            complement(3653..4081)
                     /locus_tag="KPH29_001724"
     CDS             complement(3653..4081)
                     /locus_tag="KPH29_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBG1467324.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEENIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(4098..4904)
                     /locus_tag="KPH29_001725"
     CDS             complement(4098..4904)
                     /locus_tag="KPH29_001725"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBG1467325.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(5106..5591)
                     /locus_tag="KPH29_001726"
     CDS             complement(5106..5591)
                     /locus_tag="KPH29_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HBG1467326.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(5642..6301)
                     /gene="spoIIR"
                     /locus_tag="KPH29_001727"
     CDS             complement(5642..6301)
                     /gene="spoIIR"
                     /locus_tag="KPH29_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HBG1467327.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(6446..7126)
                     /locus_tag="KPH29_001728"
     CDS             complement(6446..7126)
                     /locus_tag="KPH29_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG1467328.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            complement(7110..8000)
                     /locus_tag="KPH29_001729"
     CDS             complement(7110..8000)
                     /locus_tag="KPH29_001729"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBG1467329.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTNIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(8118..9668)
                     /locus_tag="KPH29_001730"
     CDS             complement(8118..9668)
                     /locus_tag="KPH29_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HBG1467330.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVVQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVEGIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(9762..10028)
                     /locus_tag="KPH29_001731"
     CDS             complement(9762..10028)
                     /locus_tag="KPH29_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBG1467331.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(10176..11036)
                     /gene="yabG"
                     /locus_tag="KPH29_001732"
     CDS             complement(10176..11036)
                     /gene="yabG"
                     /locus_tag="KPH29_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HBG1467332.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(11120..12058)
                     /locus_tag="KPH29_001733"
     CDS             complement(11120..12058)
                     /locus_tag="KPH29_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HBG1467333.1"
                     /translation="MKFIDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(12168..12329)
                     /locus_tag="KPH29_001734"
     CDS             complement(12168..12329)
                     /locus_tag="KPH29_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467334.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(12339..12836)
                     /locus_tag="KPH29_001735"
     CDS             complement(12339..12836)
                     /locus_tag="KPH29_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBG1467335.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(12951..13661)
                     /locus_tag="KPH29_001736"
     CDS             complement(12951..13661)
                     /locus_tag="KPH29_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HBG1467336.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            complement(13837..14367)
                     /locus_tag="KPH29_001737"
     CDS             complement(13837..14367)
                     /locus_tag="KPH29_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467337.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKSIFKNRRYSNDINELKILVNGSTGSGSIVQDFPTYEIVKNVDCN
                     IVQYSNKDERRVKAKANKMAMRLIHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(14384..14614)
                     /locus_tag="KPH29_001738"
     CDS             complement(14384..14614)
                     /locus_tag="KPH29_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467338.1"
                     /translation="MSKLVSKVKKDLEIKWLGVDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMAVVIVIFFDYIYRVLKMTRKK"
     gene            complement(14629..16023)
                     /locus_tag="KPH29_001739"
     CDS             complement(14629..16023)
                     /locus_tag="KPH29_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBG1467339.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTI
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            complement(16149..17402)
                     /locus_tag="KPH29_001740"
     CDS             complement(16149..17402)
                     /locus_tag="KPH29_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG1467340.1"
                     /translation="MENKSNFKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALSLS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGVLGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTMPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            complement(17453..18607)
                     /locus_tag="KPH29_001741"
     CDS             complement(17453..18607)
                     /locus_tag="KPH29_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG1467341.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGINGLTRDQASRKLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIKKLK"
     gene            complement(19166..19735)
                     /locus_tag="KPH29_001742"
     CDS             complement(19166..19735)
                     /locus_tag="KPH29_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG1467342.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            19938..20165
                     /locus_tag="KPH29_001743"
     CDS             19938..20165
                     /locus_tag="KPH29_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG1467343.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(20384..21127)
                     /locus_tag="KPH29_001744"
     CDS             complement(20384..21127)
                     /locus_tag="KPH29_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBG1467344.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNAASQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYITDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            21295..22167
                     /locus_tag="KPH29_001745"
     CDS             21295..22167
                     /locus_tag="KPH29_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG1467345.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            22167..22832
                     /locus_tag="KPH29_001746"
     CDS             22167..22832
                     /locus_tag="KPH29_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBG1467346.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            22853..23710
                     /locus_tag="KPH29_001747"
     CDS             22853..23710
                     /locus_tag="KPH29_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG1467347.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGSYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(23866..26412)
                     /locus_tag="KPH29_001748"
     CDS             complement(23866..26412)
                     /locus_tag="KPH29_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG1467348.1"
                     /translation="MNIFNKVALQGLKKNRTRTLVTIIGVVLSAAMITAVTTFGVSLM
                     NYMTNGAIIKYGDWHVQFLDVDSSFVQEQRVNDEVANTATFENIGYATLEGNRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSSKVATDGGVKFAVGDTISLDVGKR
                     MSENEKLGQSDPYTAGSEVFVPQDKKTYTVVGICRKPVFEESSAPGYTLITKEDKQVK
                     TDSLSLFVTLKNPHQIRSYTSSTKDHASIFNDNVLRFIGVSDNNLFNMFLYSVGGIVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGAIGIPIG
                     VAIGIVSIGLVISVIAKNFANILYVNVPLTLTLSIPAIIVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQRVYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVADLKQMSNQRSEVTDQDIIFSTQGMKDNEMLKLYDKLKTANGV
                     YEGLYQKLMTYSCVAKVSELTDDYLNYEGSHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPAEEYTGQNAKLIAVSMVTKDNNIIDIFNSSSMNVSLTPETNGKLEIPERQNVNIS
                     FVKVVPPDSPPGIRSSDVKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     AEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWFSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(26412..27098)
                     /locus_tag="KPH29_001749"
     CDS             complement(26412..27098)
                     /locus_tag="KPH29_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1467349.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(27181..28170)
                     /locus_tag="KPH29_001750"
     CDS             complement(27181..28170)
                     /locus_tag="KPH29_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1467350.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIVCEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(28157..28843)
                     /locus_tag="KPH29_001751"
     CDS             complement(28157..28843)
                     /locus_tag="KPH29_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1467351.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(29343..>29581)
                     /locus_tag="KPH29_001752"
     CDS             complement(29343..>29581)
                     /locus_tag="KPH29_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467352.1"
                     /translation="IEEPRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKMRRTAIEF
                     STEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
BASE COUNT         9618 a         5365 c         3407 g        11191 t
ORIGIN      
        1 taatttaata ttatactttc agttttagta tttaatccac atcagataat aatcttaaaa
       61 tgaccaaggt cttaaaacta tttatcttta tcagatttat cttctaaatt tttatttata
      121 tcaataacat tgtttctttc ttcttcatct ttttctaatt ttatagttaa ttgttcttca
      181 acttctttag aagaattttc ctctgtaact gcattttcaa tttcattcag aggtaactct
      241 ttattaaatg cttttacaaa ttgttctgca tctaaagttt catatataag taaagcttgt
      301 gctacatact ctaatctatc catattttct tgtaatatac ttcttgtctt atcatatgca
      361 ccatcaacta tacgctttgt ttcttggtct atctcaaaag caacttcttc agagtaattt
      421 ctcttacttg aaaaactatt acctaggaat acttcatcat cactgtcaaa tgacattgga
      481 ccaagtttag aactcattcc atactttgtt accatacttc ttgcagtagc tgtaactctt
      541 tctaagtcat ttgaagcacc tgtagataca tccttaagta ctagctcttc tgctactcta
      601 ccaccaagta aaactacaat attttctttc atttcatttt ttgtagcata gaacttatct
      661 tcaacaggaa gttgcattgt aaaccctcca gctcttcctc ttggaactat tgtaacttga
      721 tgaacaggac ttacttcttc aagtacatgg gcacaaactg catgccctcc ttcatggtat
      781 gctgtaagtc ttctttcttt ctcacttatt actttcgatt ttttagccac acctgctata
      841 acctttgtta tagcttcttc tatagtttcc atcttaattt tcttttctct ttttctagct
      901 gttaatattg cagcctcatt cattaagttc tctatatcag caggagtaaa tcctggtgtt
      961 cttctagcca agacatccat ttttacatca tcacttaatg gtttatttct tgagtgaact
     1021 ttaaatatag cctctctacc ttttacatct ggtgtaccaa caacaacttg tctgtcaaat
     1081 cttcctggtc taagtaaagc tgggtcaagt atatctggtc tatttgtagc tgccatgatt
     1141 attatacctt gatttacccc aaatccatcc atttcaacta gaagttgatt aagagtttgc
     1201 tctctttcat catgacctcc accaagacct gcacctcttt ttctaccaac tgcatcaatt
     1261 tcatctatga atatgatagc tggagcactt ttcttagcct gttcaaataa atctcttact
     1321 cttgaagcac caaccccaac aaacatctca acgaaatcag aaccacttat actgaaaaag
     1381 ggaactcctg cttctcctgc aactgctctt gatagatatg ttttaccagt tccaggaggt
     1441 cctaccataa gcattccttt tggtattctt gcacccaatt caatatattt cttaggattc
     1501 tttaagaaat ctactacctc ctgcaaatct tctttttcct catctagtcc agctacatct
     1561 ttaaatgtaa ctctagtttt ttcatcatct ttatgaacct tagcttttga tttgccaaaa
     1621 ctcataacct ttccgcctcc accttgagac tgattcataa acacgaacca aataatcacc
     1681 atgaagaata ttaacagtaa tgtaggcaac atttcgacaa accaaggtgt agatggctta
     1741 gcttcaccac caaaagttag tttcccagcc ttagcttggt ctaacacctc atctgcaagt
     1801 ttatttccca tcacttctgt tggaacatat gacttaaact ttgtatttgt atcttttata
     1861 gtaccttcta cagaagtttg atttacaaag tataatcttg atatattctc atctgttaat
     1921 tctctatata cttcagagaa ttttaaatct tttatttttt ctgtaggctt acctgagaat
     1981 tgaactatac ctactataat tataaaaaca agtagataaa aacctgcccc ttttagcaac
     2041 ttattcaata tagaagccct cctttcatac attgctttgc cctataattt gttaaaatta
     2101 atttttaata cttcttttgt gttttcatca attttataat tttcactgat tttataatct
     2161 ccaacacata ttattccttc actatctgtg attataggaa ttttacatct ttcttctctt
     2221 gggattttta aatctatgaa taaatctttc acttttttac ttcccatagc aagctttatc
     2281 ttatcacctt gccttctgct tcttattact ataccccctt ttaccttatt aaaatcaaac
     2341 cctttagatg aattgtctaa tttcatactc ttatacctat ctatgctcat tacttgggtc
     2401 tcaataattt gttttaattc ttttatttta ataaacccat tactaggtac attgtaataa
     2461 aattcaattt cctcattaac aatttcctca ttcgttaata ttatactgtc tttttttcta
     2521 taaacaaata tatttcttgg aagagttaac attttattta cttttgaatt atcttctaaa
     2581 gacattatat cttctatatg tctttggtca acaaaattag tatctcctag tatatgtttt
     2641 atggaatttc taagaactct aactttgata gcatcatgta agcaaacaaa atcgtctaaa
     2701 tttatctcta caaagccttt ttcttttata tttgaaactt ctctaaattt agcttctgcc
     2761 tctttctcaa tataatcgtt atcacttttt aaactattgc tcatcctcac tatagattct
     2821 attacatttg aattaaaatt atccttcata tatggtaaaa ggtctaacct tatcttattt
     2881 ctagtgtata tattttctaa atttgtttta tctatcctcg gatttaaatt ataagcttca
     2941 caatactcct ctatctcatt tctttccacg tctaatatgg gtctaattat gcaattatct
     3001 ctaatataat caattccctt taagcctttt aagcctgttc ctctcatgat acgcatcata
     3061 acagtttcgg cttggtcatt gagattatga cctattgcta ttttattggc tttaatctta
     3121 tccttaattt cataaaacat ttcatatctt agcttccttg ctccttcttc taatgataat
     3181 ccctcatttt cacaatattt tggtacatta atagacttta caaagaatat aattcccata
     3241 tcctcacaaa gtttagatac atataatgca tctttttgag cttctatccc tcttatttga
     3301 tggtttaaat gtgctgcata tatttctata ttaaaatctt tttttaatct atttagtaca
     3361 tgcaataaac aaactgaatc aggtcctcca gaaagaccta aaactatttt atcgcctttt
     3421 tgtattaaat tatgtttatt tatagtactt agtaccttat caaatatcat atttatccct
     3481 cttacactta gttttattca cttttaagta tactttctat aaaaaaacgc taaacccttg
     3541 tatcaatttt aagaaattaa agtttaatat aaatataata ataccaaata aattaaataa
     3601 aaggtagttt tttcactacc tttataaaat ttctggaaaa tagccatagt tattatttag
     3661 tacctatgta tatatctatt ttattcctac ctaaaaataa atcttgtacc tttatattat
     3721 agtctatttt caattttatg tgttcccctt cttgtatatt tatatctaat tctcttgtat
     3781 caatatcaat taataaagta ccttgtgggg tcacatactt tgtagtacta ctttctccac
     3841 atttaaacag cattgttgag ttaatagcac caaacttttt gatactaact tgatttttaa
     3901 gtatctttat agtattagtt atttctatat tttcttcttt ttccttgtaa ataacatata
     3961 ttccatcatt tttataaaaa attttaccaa aggcagtcat ctctatggtg tccatatttc
     4021 ctttttcatc atattgtcta gtatttatac ttatttttac actcaacgat ttttcctcca
     4081 tagattcttc atataaacta gtatttttgt atttctactt tttgaatctt atcaattttt
     4141 ttctttaaga actctcttgc tatatctgaa aatttttcag gtatatctga tacataatat
     4201 tgataagtcc catgaatttc tgtattgttt ataatatttt gcacctctaa aatctttttt
     4261 aaatcctttg cagtctcttt agctggatta acaagtttta tatgctctcc aacttcttca
     4321 cctattgttc tctttaaaat aggataatga gtacatccta acactaaaga atctatattt
     4381 ttttctttca attcttgtaa atatatcttt gctgtcaatt tagccacttc tgtatttgcc
     4441 catccttctt caacaatagg tacaaataaa ggacatgctt tattaacaat ttctatactc
     4501 tcatctattt tagatatttc caagttgtat gcttttgaac ttatagttcc ttcagttcct
     4561 attattccta caattttgtt ttttgtagaa cttacagctg ttctagctcc tgcttctata
     4621 actccaatta tcggtatatc atatttttct tgagcttctt ttaagcttct tgcagtagca
     4681 gtgttacatg ctataactat agcttttaca ccttttgttt ttaaaaagtt tatagcttga
     4741 aaagtatatt taattatagt ttctttggat cttgaaccat atggtattct ggcagtatct
     4801 ccaaagtata ttatatcctc atttggtaat atcttcatta tctcttttag aactgttaat
     4861 ccacctaatc ccgaatcaaa aactcctatt ggtttattgt tcatctttat acaacaccta
     4921 ctttacattt ttgtgtttaa caacaactta tttatcgcta aaatttatat ttatattcat
     4981 aattaatata ttattaccta aatgattatt atagtttatt ttacacaaaa aataaaggag
     5041 ctatcttaaa ttagaaaata atttctacaa aaagacaact cctttcacta cttaaaaatt
     5101 taaatttatt ttgcaaacac atgatttcct attctcatat aaggattcaa tctatttatc
     5161 catttgcaag tggctatttc tggattccaa aaatatactg ctttattagt tgggtcagtt
     5221 ccataaagag cttcctgagc tgctttacgt gctgagtctg ttggtgccat ctttattgaa
     5281 ccatcaagca caactgagaa agcatttttt tgatatatga cttcttctat tgtgtctgga
     5341 aatctagggt ccaatactct atttattact acagctgcta cagcaacctg tccctcatag
     5401 ctttctcctc tagcttctcc tgcaaccaac ttagataata acatcaagtc attatcagat
     5461 aaatttatta cttctttctt tgctatattt ttttcttgtt tgtttacttt cttaatattt
     5521 tctgttggtt catatgcaaa tacctcttta ataggcataa atataaaagt caaagctaat
     5581 agtgtagcca tcagtttact ccacctgtcc atgattaaaa ccactcctta cattcttatt
     5641 tttaaaattt tttctctaaa tccttgacta cttcaagtac tttaaactta atttttactc
     5701 tacttgtttt ttttggtgta tcttgtttta ttaacttata ttctttatca gttaaaactt
     5761 ccttcaactt atcatctgta gatttatcta tcactccatt agattcatca acaaaacata
     5821 gtggagggaa cataacacac caccagtttt ttccttcacc tttcccaatg ataatcttta
     5881 atgctttata ttcaccagct ggaagaacta tattagaata ttgtttagta gggaaattgc
     5941 tatattgtat acctaccttt acactgtaat tatatccatt tttaagaata atatttttac
     6001 ttatttcttc cagactacta tattcctttt ttataatagc ttcactttct tttatacttt
     6061 ttgaattttg aagctttggc tgtaaatatt ttattacttc atctctaact tttagtttaa
     6121 gttcttgatc ttcatctgta ttgctgtttg ctataacatg aaatcttatt aatttatctt
     6181 tataatcttt tgatatattg tctacttttt ttacctcacc atttattact attgtcatta
     6241 ttgatatgac tgatataaga cttaatatta atagtcccaa tctaatctta atttttctca
     6301 ttgaaaattc cccctcaaga taaatttata tcatcaatta tgtccaatta aatttaattt
     6361 attcataata ttttttaata ctaataaaaa aagctatctt aaaatgatta caatcatttt
     6421 aaaatagctt tttaaacctt atattttata agtctttact aatcatagcc ttatctatca
     6481 taaactgctc tgctttttct atatgctctg tagcatactt cttggatagc tcactatcac
     6541 ctttaattat agcatcaagt aagtatttat gctcttcaac aatattatgt gatgtatcat
     6601 aagcagacat ataggtaact ctaaatctat atacttgttc ccataatgag tttattagtt
     6661 ggattaattt tttattatta gttgctttaa atatacattc atgaaattct acatcaagtt
     6721 ttaataacgc atctacatcg gattccaaag tgcttttttt aaattcttct gatatatctt
     6781 taagcttttt aatatcttca tcactaattc tttcagcggc aagatatgct gctaagcctt
     6841 ctaggcttgc tcttacttct aatacatcca taatatcctt taatgacata tttgccacat
     6901 acgcaccttt tctaggcaac ataacgacta aaccttctaa ttcaagcttt cttattgcct
     6961 ctctcactgg agtcctactt actcctaatt gttctgctag ttgcacttcc attagccttt
     7021 gaccaggctt taattttcct tctaagattg cttctcttaa attctcaaag actacatctc
     7081 ttaatggctt gtaattatcc aagttcagtt tagttaaatt ctccacaaat ttcaacccct
     7141 ttttgactgc tattaacaac atacacctgt ttatattttt ttaataattc tttttttcca
     7201 attagagcat cttctttatt tttaaatagt ccaaaaactg taggaccact tccactcatc
     7261 atagaaccta aagcattatt tttcatcatt acttgtttta tatcacttat ttctttgtgt
     7321 ttacctatag taacattttc aagaatattt accatgcttt ctgatacagc ttttatatct
     7381 tcagatttta aacactctat caaatattta ttatttggtc tttttttaat attattcaaa
     7441 tctaatcctt gataaacttc tttagtagaa acgaataagt ctggtttgca tatcaatata
     7501 tttgtatcac atggtaaacc tttgatgttt gttagctttt ctccaatacc ttgagctaag
     7561 gcaggtcttc ctgatataca aaaaggaaca tctgcgccca aattaagtcc tatttctttt
     7621 aattcatctt cagataatct aagttcccat aggtgattta aacctactaa tactgcggct
     7681 gcattagaac ttcctccagc catccctgct gctacaggaa tatttttttc tatgaatatt
     7741 tctacacctt tttttatata aaatttattt ttaagtattt tggcagcttt ataaacaata
     7801 ttatcttcat ctaaaggaat atctaaacta gtgcttttta cctttatttc atcttcgtct
     7861 aattctttta tctttactat atcgtataaa tctatcgttt gcattatcat ttcaacaaaa
     7921 tgataaccgt cttgcctttt tcccaagaca tcaatggata aatttatttt tgctctactc
     7981 tttagtctta taaaatccat tctatcctcc ttgtttaaag aataatgcat cactaacaat
     8041 aatattatac tacaaaaaat aaaataatag ggtattataa taccctatta tacttaatta
     8101 ctttaaattt tttaatatta atctactaat acgacttttt tttctaaaat aagacactta
     8161 ccaggcttaa ttggttcatc aagttttata tcattaagtt ctgcaatttc attttctgtt
     8221 gtattgtatt tttttgctat attccagaaa gtatctcctt ctttgcatat atatacgatt
     8281 atgctaggag ctttagataa atcatataca ccttggtctt ctcctttgat aataaaattt
     8341 tctgctttct tatctaatgc ttcagtaaat ctcttaattt tgataatcaa gtctatctgg
     8401 tccctattca aatcaacttc gactttatca atacatgctg tattaaacac agatgctgta
     8461 tcagttagat tatccatttc tatatcatgt tcaaatggaa tttcctcact aattttataa
     8521 accaatttta aaccttcaac tgggacaaat aaaatttcaa cttttatgat tccttgtata
     8581 atgcttttat ttccttcaat ataactattt tctatagaca tggttggaca aacgcttaca
     8641 atatctttta tttgtatatc atcattatca tttcttatgc cttctctaac cataaatgtt
     8701 tcttcactac gtcttaaagt cttatttaat tgaataggtt tgtggtcaaa ttttattatc
     8761 ttttgaggcg aatatgcatc ttgtaatact tctcttgtaa cttcatctgt aacttttacc
     8821 ttacaacaag ccatacaatc tatttctaaa agacctgtgt tgctttcact attttgcttg
     8881 aaaatatgat taaaatctga catagacaac aatacttctt cagtcatacc atcactaact
     8941 ccagggactt ctacaaattg agtaaactca atacctactc tatccaactc tacaagttca
     9001 ccctcatatg tacaagctaa cggatttatt tctaatactc caccaataat aactttattg
     9061 tcagtcactc tactttcttt tactttaaca catggattta aacttattat agattgaatt
     9121 tcctctgtgt ttattgttat agtatctctt atagaacttt ctgctttttc tattccaact
     9181 atatcttgaa aacaaatttc ctttctgtgc ttttgtatgc cttccacttg cgccacatct
     9241 tttacaatgt ctaacctttg cttttcaaac aaacttcccc ttatattcat aagagctcct
     9301 actttaatct ttctctcatt cattatagta caatccatat gctctacttc tgaaaataac
     9361 atgtattcca tatcttgaac aacattgtct ttttctatta cttcgtttat atctactttt
     9421 ccgtctacat tagaaatagt atttttatca tctgcaatat agattacatt ataattaaaa
     9481 ctacctctgc aaagtatctt cccatctgcc atttcaattt ttttaagtga tatgtatcct
     9541 tctgttttta caatttcata tacatctaat tttttgtctg gtacaactgc ctccgcttca
     9601 atgaatgttt gaaattttcc aaagtctatt ctattgtcaa ctttaattac atctttaatt
     9661 aattccatag tctacctcct taagtattat aaaaatatca cactatattt atatttaagg
     9721 agcaaaaaaa aaattacacc tatttttaaa taggtataat tttaacttat ttgtaattta
     9781 tcttgatctc taaaaacttg caactttaca ttagatgtta ataaatctga gtaactataa
     9841 gatactctag gatatccatt actttcatca tctaacttta taacaaacac gcttggataa
     9901 actttttcta gtataccctc ttttgtaata atctgctttc ttcctttatt agctttcaac
     9961 aatattttct ttcctatatg tctctctaag cttactctta tcttatctag agtttgaaca
    10021 gtagccacaa tatcaccttc tttaacgttt atatgtttac ataatatcac atataaacat
    10081 ttttgtcaaa tgtttaaact aattatttta tatatttttt gcaaaaatgt caatactatt
    10141 tttgaagata tacagtataa ttatgttaat ttttgctaat gtaatattgt ttttggcatt
    10201 gcccatctaa attttcctct tgtttcagaa ccaccaattc ccttaacacc tgtaatagtc
    10261 tgctctataa cctcatcaat aggaactact acatcaattc ttgaacaagc aattttctcc
    10321 actataaaaa cagggtctaa tgcatgtatc attattcttc caggcgaact tgcataattt
    10381 gctcctgcac ttattatttt ttcataattt gattggcatg ctcctgcgaa tatgacaaga
    10441 ttatccaaat ttggttgcca tttacgtgct tcttttactg ttttaacaaa atttgaagag
    10501 tttctatagt tactcatatc ttctatatct cctcttttta ttgtcattgc atcatgacct
    10561 gttattacta gtatatccgg attatatttt tctagtagtg ctcttacttc tttatactga
    10621 ttttgctctg aaacagctac acctactgca ggtattccaa gttgagcata tacatccaag
    10681 caaattttta aatattcttt atctccatct atctgtagta cttttccagg cattccatat
    10741 gtgttaggat ttaattgaag ttttggaact gctctagtca ttcttttttg tctttcttta
    10801 gcttttctta ctgacttata taataaattt tctacatttt tatctattaa tatatctttt
    10861 atatcaggtg cttttactaa ttctaggtca tcaatataag catctgctat tactctaaat
    10921 gcaattccct ttagtatagc tattttctca ttattttcat ctacaccaaa agagactatt
    10981 ttaaatacta tatctttgtt atgtgacttt ctagctacaa tgtctcctac cttcatagtt
    11041 aaacctccaa taaattttta tcttagttta tactatgttc gatataaaac tttgtttcct
    11101 aatgtcaaaa aaacatttct tataatactt cttttatcac tcttaaagca tttttatagt
    11161 atatcttttc tatatcatct tcactaaacc cttctttttt tagaggttca tacagtttac
    11221 ccatctgaga tatatcttca atctcaactt ttgaatctat tccatcaaaa tctgacccta
    11281 gggatacaac atctattcct ccaacattta caatatgttt tatgtgtcta accatatctt
    11341 caagtttact ttcactttta tcacttaaaa ataagtggaa gaagtttata cctgtaactc
    11401 cacctttatt tgctaatact ttaatcatat catcagttaa atttctagaa tgattcatca
    11461 ttgctcttga attagaatgt gtagcaatta ttggtttaga agaaatcttt gcaatttcat
    11521 aaaatccacc atctgaaata tgagaaacat caaccaacat tcccaattca ttcatcttat
    11581 gtaccacttc tcttccaaaa tcagtaagcc ctttttctct atattctact ttattatgag
    11641 gaaagctcaa ttcattcaca tgattccaac ttattgtcat cattctaacc cctaagtcgt
    11701 aaaatttctt taaattttct aatttacctt caagtactgc accttcttca atagataaaa
    11761 gagctgtaag tttaccttct gattggtttt tcataatttc ttcatagtta gtagctaaag
    11821 ctatttcatc actatttttt ttcatttcct catgaaattt atttgccata ctcatgcaat
    11881 aatcaaaagg atgttttact tcttcagtat caacaaaaag agcaaatgtt tgagctaatg
    11941 aatccccctt tttcaatctg tctatatcaa cagaatattt attagactta agttcacttg
    12001 tttctacatt ttccattaat tttgctatag tatcacaatg taaatcaata aatttcatat
    12061 ttataccccc tgtatatttt atatttatat ctaaaaatag aaatagttgt ccttatagac
    12121 aactattgac aactattttt taatattctt tttaatatta cttaatctta ttcttctttt
    12181 aagttaggta ttactttttt aacttcttca atttgatgcc atgttttatc tatattattt
    12241 tcactaatat atttttttaa atcagttaaa gatgtaaaat ctttaactga taaattgtca
    12301 tcatcatctg caaatcttaa aataaacatg agactccctt actttccaca acatttctta
    12361 tatttttttc cgcttccaca tgggcatgaa tcatttcttc ctatttttgc ttctttaact
    12421 attgttttag acttcttata agcctttatt atttcttttc tttggtctgc tggtagtata
    12481 tcttcccaac cttgtaaatt atatagccaa tgagcctcaa cagttaccat attgttatat
    12541 aatttttccc aatctatttt tatagaaact tctttatcag cttctacttc ttctaaaact
    12601 atatcttctt ttaagctttc acttattcca tctatgaatc ccatgaaaaa ttcattagta
    12661 gttccaaatt tttcagccaa ttcacttact ttaccttcaa caacatccat tttattattt
    12721 aatatttcat tgtatattcc agcttcaact ttcaaatatt cttcccagaa ttttacttct
    12781 gcttcttgac tttcatgatt atcacttaaa tttctccatt cagtatataa actcatactt
    12841 tttcctccta agtataccta tatttttata gtttttatag tataaaatct tttagttaga
    12901 ttggaagttt cttctttttg attataccat attctattta aagttaactc ttatattata
    12961 tcataattaa acaattcttt taatactttt atagcatact cttggtttat aaaataaggg
    13021 cttgtcgcaa ttgttattac tttggctttt ttaattaagt tttttatctt gtaaaatttg
    13081 agttcaaatg gtatatagtc catatcactt gaaaatatat ccaaatctat atcaagaaca
    13141 aactctgatt ctacttctaa atcaaatcca tacagactat ctattattat taattctgaa
    13201 aatatgttat attttaaagc aggttttata aaacttccca catttaaaac ttcatttgta
    13261 tacctaaata catcttttag attatttaca tctacatcat agttctcagg ctctctagta
    13321 tccttatgtt ggtctacatg cactaacttg catccttttg taaattcacc ctttaacaaa
    13381 gattttgccc aaaaataaaa tgcatggtta tgattgtcaa acacatatat ctttttattt
    13441 cccaggacat attcgaccat attctccaat ccttttgctt ttatttcctt attttcatct
    13501 acttcattaa ataccacctc attgcctaac cttacatcat ttaatgtacc atttataagt
    13561 tttggaacgt atatgctctt attttctctt ttatcatatg aaaatacatt atttcctact
    13621 ggtttttcta tataaaatcc actatattca taattttcca taaaacttta tttcctccat
    13681 tgttatataa atatataagt tataatttta aaaattaaaa tgcacttatt gtaaatcata
    13741 ttatctatta tagaaaacat cttatataac taaatataat atcttctctc aatatgtatt
    13801 atacaataag tgcaaaatat atttatataa aatatactac attcctataa caaaaccatc
    13861 agcttcaatt ggaaggtaac taactcttct attcaactcc acctttccaa aaaatactac
    13921 ccaatatggt ctaaaaatac tttcaatttt aactatttca atctctggga ttcctcccat
    13981 aaacctatga attaatctca tagccatttt atttgccttt gcttttactc ttcgttcgtc
    14041 cttattacta tactgcacaa tattacaatc cacatttttt acaatttcat atgtaggaaa
    14101 atcttgtact atactaccag aacctgtaga accattcact aaaattttta attcatttat
    14161 atcattagaa tatctcctat tcttgaaaat ggatttcgtt ttgtgaattt taagtgtgat
    14221 aattttaaac tctatataat gaagtctaac ttcatgagca tatttgttct ttaatattag
    14281 cttactaaat aaagaatttt tcccactttt agctatctct atagcttctt cttttgtaat
    14341 tcttataggt aacgctctta tatccataaa atcattctct ttcttatttt tttcttgtca
    14401 tttttaaaac tctatatata taatcaaaaa atattacaat tacaactgcc ataactccat
    14461 atggaattat ttttccaact ataatatcat ttctagtcaa tataccataa aatataaatc
    14521 ctgttattaa aaagtacaat aatactagaa aaccgtctac tccaagccat tttatttcta
    14581 aatctttttt taccttagac actaatttag acatagcagt ttctcctttt atagattatt
    14641 ttgtgtattt aaattttcac ctattctatt accaatcatc catgcaataa atgaaaaagg
    14701 tatagctatc acaagaggct ctaactcaaa gctctgaact ggaaatattt taaaataacc
    14761 aaaagtcact ccacctacaa tcattcctaa aataccaccc atagatgttc ttcttccatt
    14821 tccaagaagc ccacctacta taggtacaaa tgccgaaccc atccaaatac ttcctacaaa
    14881 tatacatgca tcatatacat tttcaaatct aaatgctacg ctaagcccca taactgctgc
    14941 taaacataca cctattctag taaactttac taattgtttc tgagataact ctttttttgt
    15001 aattcttcca tatatatcat ttgctaatgt agctccagct gctagataat agctatccaa
    15061 tgttgatatt atagaaccta aaattcctat tataaatatt actcttattc cttgtggtaa
    15121 tgtatctaaa actaaagtta tatatccagc ggctggagcc atctctggat aagtagctct
    15181 tactataagt cctgttatag ttaacaccat gtcaaaacat atccatagtg aaaaacaagt
    15241 taataaggct cttcttccaa ccttaggaga atctgctgct gaaaatcttt gataaaaagt
    15301 tgggtctgaa taagctccaa aagaatatac aattaacatc aatgctttca tagctgacat
    15361 accttttgta gggtgcataa gctgtggtgc atctttgagt gcttctgtga gaccttgaaa
    15421 tcctcctatt tgttcaaatt gacctggcat aaccatagtt aaaccaaata ccatacaaaa
    15481 aaacaatacc atatctgtaa tggcaactcc cataagccca ccaagtactg taagtacaac
    15541 gactactcct accattatta atcctattaa ttctttatct attttccatg ctacgttacc
    15601 tatagttccc atagcagtta tttctccaag ttgtgcacaa tatataaaca tcaaaattgc
    15661 cccaattata gcaacatgct taccataaaa actttccagt aaatcaggaa ctgttatatt
    15721 ggttcttata tgaatccttg gaccaaccca caatgctaaa ggcattctcc caatatgtgc
    15781 agctattgac caaactgccc atgcagatat tccatatata gccgcatact ctacactacc
    15841 tactgtacca actcctccat accatgatgc aaccaaagtt cctactatta aggaccatgg
    15901 taaacttcga tttcctaggt aataactttc catatctttt acatttctag aaaaataata
    15961 acctattcct aataatataa atgcaaatat aactataatc ccattttccc aaaatgttaa
    16021 catatctaat ctccacattc ttatatttta atttttaact ttaatatcta aatatgaaca
    16081 actctaccca tttccataaa aataggtaga gttatataat aattataagt atttaagtaa
    16141 tcttgttatt aagcaatttt attgtttaaa cctttatttt ctcttttatt tattttattt
    16201 aaagcataag tagcaaatat ccctataact gcacatacta cagataaaat aaatatatat
    16261 gtatatcctg tttgtccata tttatctaac caattacctg caatagtgta ataaaacgca
    16321 tctggagaaa atcctataaa tgaagcaaat ccaactactg ctcctgtagt ttctaaagat
    16381 atattagact ctgaaacaga tgcaaagtat atacctctaa atccaaataa aattacactt
    16441 agtattatca tgtttattac agctatccat ataaatcctg tactcttagg aataaccaag
    16501 taagcagctg tacttactgt cattaatata aatcctacaa acattactct tatagatgaa
    16561 ccaactttat ctgttataac accagcaact ggagatgctc ccatttttat aacataagtt
    16621 cttattattg ataaagcaga aactagagtc attggcatta cataaacttc tgataagtat
    16681 ggacttaaat aagttaaact tgagaatact aaataagttg aaaatattat aagaccaatt
    16741 aaccaagcct ttggcattgt aactactttt aacaatgact taatacttaa tccttcatct
    16801 gatgctccac tatctttttt ctcttccact aagaaaaata gaagtatacc acatattata
    16861 gatgctgcag aatataacca tactacatat ttgaatccta ctgttatttc agcaaattta
    16921 ccaaagaagt aaagacctat aaaagaaatt aacgtacctg atacacctga taacccttca
    16981 taaaaaccaa atattcttcc ttgttctgac tcacttcctt gcattctaac aaccttaaca
    17041 gatgctgaga agaaagttaa tattgttgta aatccccata atacaaatat taagagtatc
    17101 atactatatg atgggaatgt agagaaataa aaaccaagta ctcctgaagc aattaaagaa
    17161 aaagttatca gctttctaac cgagaatcta tctgcaaacc atcctcctat aaaataagat
    17221 accattccta ctaatccata taaactcaat aagttcccta tttgtgtatg gctcaatgat
    17281 aaagcttgtt gcattgggtc ataaaatgat gatttcatat atggtaaact atacatagcc
    17341 gttgttccaa atgccaatat gaatattatc aagaatcttt taaaattact tttgttctcc
    17401 attaattttc tctccttaaa attttattgt ttataatctc attcatgtaa ttttatttta
    17461 atttttttat tgcttgttta aagtcattta ttatatcatt tgaagattct aatcctacag
    17521 ctattcttat tagattttct ccattcattt gaactaatgt atgctcttcc cctaagcttg
    17581 ttgctattgt tgcaagctct aaagagttca ttaattttct actagcttgg tctcttgtca
    17641 atccattgat accatcctta agtttaaatg aaactacacc tccacctaaa ccattcattt
    17701 gagttttaca aagctcatat tgaggatgag attttaatgc tggatacttt accaattcca
    17761 cattctcttc ttcctctaaa aactctgcta ctgctaatcc attttcacaa tgctttttca
    17821 tcctcatatc taacgttttc atacctctta aaatcatcca tgcactcatt ggtggtaaag
    17881 atgctcctgt aaaacaacat aaacttggcc atctaactaa atctattaat tcttttgaac
    17941 ctgctactgc tccccctaaa gtatccccat gtccatttat aaacttagta agactatgta
    18001 ttactaaatc tgcacctaaa tctattggtc tttgtacaat tggtgtccca aaagtactat
    18061 caactatagc taagctttta ttttcatgag caatttctgc tattgttttt atatctacta
    18121 agtctaatga tggatttaaa ggactttcta tataaactat tttagtattt ggtctcattg
    18181 cttcttttac attttgtgga tttactgcat ctacaaatgt aacttcaact ccaaatttag
    18241 ataacaactc agacatgaat aatttagtgt gtgaaaatat tccatctgaa ctaactatat
    18301 ggtcaccact ttttaccaat gctaatatag tagatgttat tgcccccatc cctgatgtac
    18361 tagatatggc actttctgca ttttcaagca tagccaactt agcttctaac tctgtaagag
    18421 taggattaca ctctcttgta tacacatatc cccatgtcat acacaactct tcaaaatgct
    18481 ctactgtctt tgcagcaaat gttgctgttt gacatattgg tgtagccaat gcacctgttg
    18541 ttgggtctgc actatgtccc caatgtatta tttttgtctc taaatcttga ttaatattgt
    18601 tactcatttt gatttcctcc cattttgact tatttataca acgttttccc tgccaaattt
    18661 aatcaattta gcttagtctt aatacatttt ttcaagtata cttttttatt tgttataatt
    18721 tctaaaaata tgacacttat tttgtttttt tacagttcta attataaact ctatagtaac
    18781 tatagagtca atatgttttt gatatttttt ttacaatgtt taaaatagtc agtacaaaaa
    18841 taggtatatc ttagaataac taaagtcatc ctaagatata cctattttta tatcacaata
    18901 taaataatta catataagca ttagtttttt tatcataaat agatttttca agatttatca
    18961 tcttttcata aatatattat agttaaatat tttaatatta cctaacatct acaataaaat
    19021 atactcttaa aataaaatta tataattaaa aatactctat aaaagctaaa aagttttcta
    19081 ttcgctacat ctaaaaataa ttagatagag gtataaatgt atttagttta tttatacctc
    19141 tagtctttta taaattatat ctctactaaa ttgatttttt agcacgaata agtgtgctct
    19201 tggagatacc agtcatcttc acaacctctc tatatggctt agaatttaat aaccccaatg
    19261 catattgtat ctgttgattg ctgtatttct ttggcctacc atctttaaag tcctttttct
    19321 ttttagcaaa ttcttttcct aattgagtac gttctattat catatttctc tcaagctctg
    19381 caactgcaag taatgtagtc aaaaagaatt tacccattga agtattttct aaaagaccta
    19441 tatttaaaat atgaacactt acttctttag caaataaacc ttctattagt tgtatccctt
    19501 ctgatgtact tctagctagt ctatccagct tagttgcaac aagtaagtct ccctttttta
    19561 gagatgatat aattttttca aatttaggcc tgtttgtagt agtgcctgta taagtttcct
    19621 ttattatttt agcatcatta tatttattca aaatttcttg ctgttgctga tacaaactgt
    19681 tgttgtctgt ttgatttttt gtacttacac ggcaatatcc atatataata atcatcactt
    19741 cctaacataa agattttgta cagaagttac ccagaagctt gtttttactt atctttatcc
    19801 attttactaa aacgattttt gggtgtcaat actgttaact tttgacacca taataattcc
    19861 tttataattt gtttgactat gtcaaaaatt accaatataa tgaaattaat gagtttatac
    19921 aaattggagg aaataatatg aatagtctgt taaaagaagc aatagatgaa ttaaaaaaca
    19981 tccattgcgg ggaagttttt ttattaaaag aattgttcaa aggctatgaa tggaacaggt
    20041 tggagatgaa tattagattg aaattaggaa aacttttttt gtatgaagtg gtaactaatc
    20101 ccaatttaaa tgtagaggtc atcaataaaa attctgctta tcagcaaatg tataaaaaaa
    20161 aatagtgact taaatgtata taatagtata atcttcttat tgattcaaaa gttaaaactt
    20221 ttaatacaaa acaatcattc tattttaaca tttatcaata tgtctaaatt tatataatta
    20281 acacatacga atagtattgt ttaaatcaca ttatttaaga tagataaatc ttaaccctac
    20341 tccagcataa atactaaaat agggcttaga attatctata tttttatccc ataaaatgct
    20401 tagctcttcc tcttgattga acatatatat ctggggaaat acaatagaag cgtaaattta
    20461 taaaatttcc aaagtcagaa aacttcagtt caaacttagg tataccaaaa ctttcaggtg
    20521 ctatttcatc aacgccaaaa tagatgataa ttccattatc tgtaatataa aatgcttggt
    20581 cttcaggtat aactaactca acattagaat aatacatttc tctattttca tttatttgag
    20641 atttaatgta atcagtaatt atattaatat aatcaactcc atctctaaaa atgtcattaa
    20701 gtaaaagttc ttttccagtt gtcaaatcta tattgtaatt attcaatgta ttgtatttag
    20761 gcccatagtt attagcaaac cccataaggt ttaatatcat actcagtaca taatttttat
    20821 taaaagtaac tgcgtaactg gtagataccc aatagctttg ttttggaagt gaattttgag
    20881 aagctgcgtt attatattct cgttcttctt cctgtaagcc atttttaaaa ttatttacag
    20941 tcattcttac agcattgttt atgttattta aaataactgg gtttacatac gtggattgat
    21001 ttctaatatt gtatataaca ggatacttta catcatatga aaaatactta cttctttcaa
    21061 aatcttcatt aaattgtaca tataatatat tatatgtaca ggtatttgta gattgataat
    21121 tttccattaa aatcctcccc cttttataaa aaaatcagat acaatataat atgcttaaaa
    21181 cagtaataat gttaatagta aatgtaaaga atagaaagta tctaaaacag caaaaaatga
    21241 ctaattttgt ggtataatac ataggattga gagttatttt ataggaggaa aaatatgtta
    21301 ttattagcag ataaatggaa agattatgaa cttatagata tgggaaatgg agaaaaatta
    21361 gaacgttggg gaagttatgt acttagaaga ccagacccac aggttgtatg gccaatggaa
    21421 tctgaatggg ctttatggaa aaaccctcat ggacattatc acagaagcaa taaaggtgga
    21481 ggtcaatggg aacacaaaaa aagatatcca gaacaatgga ctataaatta taaaaactta
    21541 aagtttcaca ttaaaccaac tggctttaaa catactggat tattcccaga gcaagcagct
    21601 aactgggatt ggatgataga gaaaataaag aaagctaaca gacctataaa agtgttgaat
    21661 ctttttgcat atacaggtgg agctactgta gcatgtgcat ctgctggtgc agaagtatgt
    21721 catgttgatg ctgcaaaagg tatggttaat tgggcaaaag aaaatataga attatcaggt
    21781 cttaaaaatc aaacagtaag atttatagtg gatgatgttg taaagtttgt tgaaagagaa
    21841 ataagaagag gtaaaaaata tgatgcaata ataatggacc ctccttctta tggaagaggt
    21901 cctaaaggag aagtatggca aatagaagaa aagttataca gttttgtaga tttatgtatg
    21961 ggagttttat cagataaccc tttatttttc ttaattaatt cttatacaac aggattttct
    22021 ccaatagttc ttgaaaatgt attgtctaca acaataggta aaaagacaaa tacaggagat
    22081 atatacggtg gggaattagg aataccagct tccagagatg gtaagatact tccttgtggt
    22141 atatttggaa gatgggagaa aaaataatga taaaagtcat atatgaagat aaccatttat
    22201 tagttgtaga aaaaccagtt aacatattgt ctcaaggaga taatactaat gataaagata
    22261 tggtaaatct tttaaaagac tatattaaaa aaaagtataa caaacctgga aatgtatata
    22321 ttggtttaat acatagattg gatagaccag taggtggagt tatggtgttt gcaaaaacat
    22381 caaaagcagc atctagattg tctgagcagg taagaagtaa gactttcaaa aaaacttatc
    22441 tagctgttgt tcatggcaag ataaaatcta actcagatat actaaaagat tttttatata
    22501 aaaataaaaa aactaatatg gtaactgtag tgaaaaaaag ccataaagat gccaaagaag
    22561 ctgaacttaa ctataatgta ttagatttta aagaaaatct aagtttagtt caagttaatt
    22621 taaagactgg tcgttctcat caaataagag ttcaatttgc aagcagaaaa catccattat
    22681 acggagacca aagatatggc aaagaaataa ataagattgg acaacaaata tctctttggt
    22741 ccaataagat agaaatttgc catcctacta caaaagaaaa aatggaattt acatgtcatc
    22801 cacctgacaa atatccatgg aatatatttt aaatctcaaa tgaggtgatt atatgagtaa
    22861 aaaattttca ataggagaaa tgtctaaact tcataaaata agcatacaga cactaaggta
    22921 ctatgaccaa atagatttat ttaaacctgt tcatgttgac caagaaagca attacagata
    22981 ttactcaatt gaccaatttt cacaattaga tattataaaa tatttaaaat atttaggtat
    23041 gcctttaaaa gaaattaagt caaagcttga tgaaaataat gaagaaattc taaaattact
    23101 aacaaataaa attaatatag tggatgaaaa aattaaagaa ttagagctta taaagaaggt
    23161 actaagcaca aagaaagaaa ctatggaaaa aggtatgaaa gtagatgaag ttggtaaaat
    23221 agtgagaaaa catattccac gaagaagtat cttatctata gagtatgaag aaggattaag
    23281 tagtgaagaa actatggaac tttccagaag acaaatagca aatatattag aagataatat
    23341 atctgtgttc tatggtggag taagtggact tgtatctata gataaatttt taaaagaaga
    23401 taaagttatt tatgacaata gttttgttac tgttgaaaga gacttatttg atataaaatc
    23461 acaaaaacat ataacagaaa tttcatctgg tgattttatt tgtatgacat ataaagggtc
    23521 ttacaaagaa aattataccg cttttaagaa actggttgat tatgttgaat ataaaagaat
    23581 acctattgaa aattatatat atgaaatacc tataatagac cctctgtcta caaacaacga
    23641 agaggaactt ctaacagaaa ttcaaataca agttaaaaag agagaaattg acttaagatg
    23701 tctagtgtag taatctagtt tacttacaca tcttaatata gttgtctatt atcaacatat
    23761 gatttttcta gatagtgatt cctaacagtg agattttcaa aaaataggag gaaatatggc
    23821 taatgctaat tccctcctat ttttaattaa tccatatcat tttaattaag ccatgtcatc
    23881 acgaagtgca tcaataatat tttctttttt tatcttatta atagtataca acattgtaat
    23941 aaatactata aataaaacac taaatatact aattccaata ctaaaccatg gaatcacaaa
    24001 atcaatatca tctgtaacca ttactttatg aattagccat gaagaaatta ctgctatagg
    24061 aagtccaaag aacaatgctc tcatgccata aaaagcacac tcaaaattca tcatcttttg
    24121 gaaatcatgc tcagacattc ctacagagcg aagcatggca agttctctac gacgtaactt
    24181 aatattagtt gaaattgtat taaacacatt ggcaatcgaa atcagagaaa tcataattat
    24241 gaaagtatag gcaaaaacat tggcaataaa aatgtaatta cggttctcat ccatcattcc
    24301 atacatattg tacagattgt actcattagt aactccctca ctctcaataa tttgctccat
    24361 ttctgcaact gactgagtag gatttttcga ttgaaagctt aaactttcaa ttcctgtaga
    24421 aacaccagga atctcaaact tatctttggc tgaataagga gccaatactc tgaagcttgc
    24481 tttcttcaca tctgaacttc tgattcctgg aggagaatca ggtggcacta ctttgacaaa
    24541 tgaaatattc acattttgtc tctcaggtat ctccaacttg ccattagttt caggagtgag
    24601 actaacattc atagaagaac tgttgaaaat atcaataata ttattatcct ttgttaccat
    24661 agaaacagca atcagttttg cattttgtcc agtatattct tctgctggta atccaagact
    24721 tttaataata ttcagataag ttctatcatc aagaaattgc atatctactg gtaattcaag
    24781 catttcactc atagaatgtg aaccctcata gttcaaataa tcgtctgtaa gctcacttac
    24841 tttggcaaca catgaatagg tcataagttt ctgatacaaa ccttcgtaaa caccatttgc
    24901 agtttttagc ttatcgtaaa gcttcaacat ttcgttgtct ttcatacctt gtgtagaaaa
    24961 aataatatcc tgatcagtaa cctctgacct ctgatttgac atctgtttta ggtctgctac
    25021 taaagcactt gtagatataa ataaaactat gcttaaaaca agcgacaata caatacttcg
    25081 ataacgtttc ttgtttcttt taaagttttt catagcaaga actccctcaa gaccataaac
    25141 tcgctgtgtt agttttgatg tcttcacaga tttagattca atttttacct catttgtctg
    25201 acgaatacac tccatcactg gtgtgttggc agcctttctt gctggtatgt aagcagaaat
    25261 taagattgta accatactta ccactgctgc aacgatgatt gcagggatag acaatgttaa
    25321 tgtcaacgga acgtttacat agagaatatt tgcaaagttt ttagctatga cagaaatcac
    25381 aagtccaata ctgactatac cgattgcaac accaattggt ataccaatcg caccaatgca
    25441 aattccttca aatagcaccg aatttcgtaa ctgttttgct gtagctccaa cagatgaaag
    25501 tattccaaat tgatgtgttc gctcgttcaa tgacatgtta aatgaattat atattagaaa
    25561 aattgagcct agcatgatta tggcaagcac aatgccacca accgagtaca aaaacatatt
    25621 gaacaaattg ttatcagaaa cacctatgaa gcgcaacaca ttgtcattga aaatggaagc
    25681 atggtcttta gtgctacttg tgtaagagcg aatctgatgt ggatttttca atgtgacaaa
    25741 aaggctaaga ctgtcagtct tgacttgttt atcttctttg gttatcaagg tatatcctgg
    25801 tgcagaagat tcttcaaata cgggctttct acagataccc acaactgtat aagttttctt
    25861 atcctgtggc acaaaaactt cactcccagc agtgtagggg tcactctgac caagcttttc
    25921 attttcactc attcgtttcc ctacatccaa agaaatagta tcacctactg caaatttgac
    25981 accaccatct gtcgcaactt tacttgaaac aagaatctct ctactatttt caggtaatct
    26041 tccagaaatt aaagttatag gcaaagtatc aaaagcttct ttgctaaatc cagcaataaa
    26101 aagatatggt ttatctggat ttctgttacc ttcaagtgtt gcatagccaa tattttcaaa
    26161 tgttgcagta tttgcaactt catcattgac tctttgttcc tgtacaaaag aagaatccac
    26221 atccaaaaac tggacatgcc aatcaccata ttttataata gctccatttg tcatatagtt
    26281 catcaaagaa actccaaagg tagtaactgc tgtaatcata gcagctgaca gaacaactcc
    26341 tataattgtt acaagagttc gagtgcgatt ctttttcaat ccttgcagag caactttgtt
    26401 aaaaatgttc attaacgatt caccttcctc tcatcacgca ccacttttcc atctgaaata
    26461 cagataattc tatctgcctg taaagcaatg ttttcatcat gtgtgacaat aatgagagtt
    26521 tggtgatatt tactatggct ctctttcagt aactggataa tctcttgtcc atttttactg
    26581 tctaagctac ctgtaggctc atcagcaagc ataactgctg gtgcattcat caatgcacga
    26641 ccaatggcaa cacgttgctg ttggccccca gaaagttgat ttggtaaatg tgtttttcgt
    26701 tcttttaaac caagtaattc taacaagtca tttaaccgtt cttgattgac cttacgctta
    26761 tccataagta taggcaatgt gatattttcc actacattta atgtgggtat cagattgtga
    26821 aactgataaa tcaatccaac ttgtcgccta cgaaaaatag caagtttttc attgctttgg
    26881 ccatatacat cttgcccctc cagatatatc tttccacttg ttggtacgtc tactcctgca
    26941 atggaatgta gtagtgtaga tttaccagaa ccagaagaac caatgattgc agtaaactcc
    27001 cctttttcaa tagtaagtga gacatggtca agtgcagtaa ccttattttc attcttacca
    27061 taaaccttac ataaatcttc aatctttaaa aacgccatta tataagtctc ctttcatatt
    27121 ttataaatat atcatagaac tttcaactta catttaagtg acatttaaat gagagattcg
    27181 tcactttgga aaacgaataa caaatattgc accaccttgt gggtgatttt gtgcagtaat
    27241 tgttcctcct tgtctcataa taatcgtctt acaaagtgcc aatcctattc cataaccagc
    27301 tgtgcttgaa tttttcccac gataaaatct gttaaataga catggtaagt cttctttttt
    27361 aaagcctgct ccgttgtcat gaatagtaag ttgagtaaac aaaaagttgt cctcacaaac
    27421 gatatcaatt tttccattat cacccacact ctccatgcag tttttaaata tattttgaat
    27481 tgcttctgaa agccaattta aatcacccaa aatccttact ccttttggta tatcaatatg
    27541 taaagtgata ttgtgcagtt ccatgggaat caaaagttga tgaagtgcag aactaatcaa
    27601 attattcaca tcaatttgtt ctttttgaaa gactacaata cctgcatcta aacgtgataa
    27661 ttttaaaagg gaagtaatca gccagtccat tcgtataagt aattcctctg tttctcgtat
    27721 caactctttt ctctcatctt catcagaagt attctctaac agtgacaaaa taagagttac
    27781 tgatgtaaga ggagtgcgaa gctggtgggc tatgtcagct agtgagtcag caaggtgttc
    27841 tttttctttt ttcagtgcgt aattttgctc tctaatacgt aacgtcattt tagttatttc
    27901 actttgtaga atagaaagct caccctcatc tgattcagca atatacagat ggttagcatt
    27961 atgaagcaca agattaattt gttctgaaat ctgtgcaata cttttgtatc gatttttagt
    28021 aaacacaaaa aatgctattc caaaggtagt ggcagaaaca atagaaagta ttccagtcat
    28081 aatactgatt gcaaatccca gggctacagt gagaatagta attaataaga ataaaatagc
    28141 aaattttcga aactctctat ttcgaaacat aacttccccc caatctatac cctgtcccac
    28201 gaacagtcaa aattatttgt ggacttgaag ggtcatcttc tatcttctct cgcaagcgtt
    28261 tgatgtacac agtcaatgtg ttgtcattaa caaactcacc tgccgcatcc cacaattcat
    28321 caagtaatct taccctcgta ataatacttt taggattgct aataaacacc aataacaaac
    28381 ggtattctaa agctgaaaga aaaacctcat tgccattctt tttcacaaca ccggttgccg
    28441 tatctacata aagcccacaa atttcaaaag ttgacccaaa acgcccactt tttcgtagtg
    28501 ctgttctaat tcgcgcaatc agttcacgtg gacgaaaagg tttagtaata tagtcatctg
    28561 cacccatatt cagtccagta acaacacttg attcatcacc agaagccgtc aaaaagataa
    28621 caggaatgtc ttgcttttct ttgatttctg tgcaaatcgt aaacccattt ccatcaggta
    28681 aagaaatatc aataagtgcc aaatcaaatt tattcttagc aatctctaca agtgcttcac
    28741 ttcttgtgga tacatgggtt actataaatc cttctgaatt aagcaaaaga ataagatttt
    28801 tggcaattgc tttatcatcc tcaactaaaa atatccgttt catttacttt catcctcctt
    28861 tattttatta gttttatata ttccagcaga gcaatagtac actactattt tacttatcat
    28921 tacacaattt tcagtagttt cttttgttct tatataatcc atgcataata cctccattga
    28981 tttattatat ttcttataac ataaatacac aagataagac aaaaaacata atgtaagtgc
    29041 aagtttttta actcactaac ttataaacat acactcctac taacactact ttcttgatga
    29101 cacatcttac aagcttatat tcatacatca atgaaaaatt aaatttatcc atttattaaa
    29161 ataaaaggca atagcttcta tagctaatgc cctttatctt aaaatgattt aaatactata
    29221 caatttttat aaatggctat aaataaactc gtatagaata taaaatttat ctacagtctt
    29281 ttgttataca aaatttataa cctcttatag aacaaattta ccactttgta aacttatcac
    29341 ttttactcag ctatattata aacttttaat ttatctttgt ttaaatgttt cttcttaact
    29401 tctactaaag cttttagcgt atccaaagat gtcattattt cagtagaaaa ttcaatggct
    29461 gttcttctca tcttaaatcc atctcttgta gagtcatttc ctttagttgg tgtatttatt
    29521 actatatcaa cttgtttatt tcttatagca tctaatatgt ttggtctagg ctcttcaatt
    29581 c
//
LOCUS       DAELXX010000041        29366 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.041, whole genome shotgun sequence.
ACCESSION   DAELXX010000041 DAELXX010000000
VERSION     DAELXX010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29366
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.041"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(30..1385)
                     /locus_tag="KPH29_001753"
     CDS             complement(30..1385)
                     /locus_tag="KPH29_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HBG1467353.1"
                     /translation="MNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREKI
                     YNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDKA
                     AEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMDA
                     VKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGVS
                     GPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSLA
                     PTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIPV
                     SEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGMI
                     NNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPISTNSSELFTQRGGRIPAPIHSFK
                     N"
     gene            complement(1411..1680)
                     /locus_tag="KPH29_001754"
     CDS             complement(1411..1680)
                     /locus_tag="KPH29_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBG1467354.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
     gene            complement(1970..3910)
                     /locus_tag="KPH29_001755"
     CDS             complement(1970..3910)
                     /locus_tag="KPH29_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467355.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCNMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKDIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLRDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAKDSGYNIVKCCHRGIGVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNQLDIDVEWDFTDYYEKLEKGENFDITGKFKTEMLNKEKIIINDDVV
                     PILNVSVIEKKTIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNSDEKQPNTPKDD
                     VESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNPITENDDNYDLSSKSVFGTNEH
                     SFPYRESISNKNQDTNKNKSNISDYDGIINNNYKLESKEYNLKKDIIFLFCILTPIMI
                     GSILIVNPDTRKSIIKFIKLKK"
     gene            complement(4094..5569)
                     /gene="spoIVA"
                     /locus_tag="KPH29_001756"
     CDS             complement(4094..5569)
                     /gene="spoIVA"
                     /locus_tag="KPH29_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HBG1467356.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIIQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(5741..7036)
                     /locus_tag="KPH29_001757"
     CDS             complement(5741..7036)
                     /locus_tag="KPH29_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG1467357.1"
                     /translation="MSVEVNKPSIWKNFKVLIILFVAGAFIYALPYLKNYYYDTFAQA
                     FNLTNTQMGSLGSIYGLLAMVSYLFGGFLADRISARKLLSGSLIITGISGLVLTLYPP
                     YIVVFLIHALWGITTILTFWPALVKAVRMLASENEQGKAFGFMESGRGITNAIHLSLV
                     LILFGYISSKVSDKSGLTAIIVLYSAINVIVGVLVFFKLKDNEKEVSAKFDKEAFFAV
                     IKMPHTWIITIIMFCSYSANMSFYYFTPYSTEAFGATVVFASAISILAQYCRPVASAA
                     SGLLGDKVGSSKIISYGFILMIAGLLGVIFTPTKSSMIYILLIGCVAIYVSMYAIQGL
                     HYSLLEEGNYSLSISGTAIGIVATLGYLPEVLCPLAAGKILDAFPGVTGYRYYFIVLT
                     IVAIIGLVFTFIWMNMTKERRKELIEMNNKSNENTTKVS"
     gene            complement(7220..8629)
                     /locus_tag="KPH29_001758"
     CDS             complement(7220..8629)
                     /locus_tag="KPH29_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG1467358.1"
                     /translation="MKKKIGFWDLVFMNISALFGIRWIAKSTASSFGLGLGSIPVWLL
                     FAFIFFVPASLICAELAATYPKDGGLGEWVKQAYGEKWGFMVSWLNWTSKIFWYSSFL
                     TFLAINIAYMLGNPDLSNNKMFILALSLIIFWLLSLVSTRGMAFGKFFTNTGALGSTI
                     PAILLIVMAFMSVVILKKAPSASIYTIQNIIPKIDANSLVSISAIIFALSGAETTANF
                     ITEMDNAKKNFPKAILTVAVLIGGIYILGSVAITMILPPDKIAASTGILDALAKVAQD
                     LGIGSWFIRIVAFGITLSVLGALILYIAGPVKMLFGNVREGVFPKQLTVTNKYNIPSN
                     AVIVQAIIVSLLLVGTNLLPSVDNIYNVLVTMTALTALFPYVLLFTSYIRLRKTRPNE
                     ERPYSIAKSDSVCINIARMVLVVTVVGILFSTAPVMETLKDNIIYEIEMIGGGLFVII
                     TGLMLWKNYEKKISNNSGR"
     gene            complement(8681..9820)
                     /locus_tag="KPH29_001759"
     CDS             complement(8681..9820)
                     /locus_tag="KPH29_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG1467359.1"
                     /translation="MSNNMRLERLRKYMNEKSIEIALIFEPDNQFYISGFKAITYSRP
                     IVTVVTQDKIELIVPGLEELHAKEVAKVDNVYVYYEIPEMKEHGISHKHYLDIILNKY
                     PKGTTVGIEKDIVSASFSEYITDKSFAIKDVGSKIFEMRYVKDAKEIEFLKIAGYLSD
                     IGIKGSLENVRVGMSELEFDVAGDNALLKYVSENYPDTYIGFANWTCSGIDRTAQPHL
                     DSNTRILQRGDIVIHSRQVWYENYRAENERTFIIGKPTERQKEVFKIAVEAQQAGLDT
                     IKAGIPARMVDEAARAVVAKYGLELYSNHRIGHGLGLSEHEEPYLRFDNELILEEGMV
                     FSMEPGIYIPGVGGFRHSDTAIVGKNGATIITNYPRSVEELILDI"
     gene            10139..10789
                     /locus_tag="KPH29_001760"
     CDS             10139..10789
                     /locus_tag="KPH29_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG1467360.1"
                     /translation="MTNKELQKKRILMYFIEAAQNIMENEGIENITLRKVADMAGYNS
                     STLYKYFKNLDHLISFASIKYFKDYNLNISRCIENVNDEYKKYIIMWKLFCKHSFDNA
                     QAFYQVFFNLSSDELSYITKQYYDMFPEDLGIHDSDISLMITGESIKERNKILLNNLV
                     DTGYIPAKDLDIINDIIISFYQNLLFLKKNTGKNSGDDELTMKMISSIEYIIKHAK"
     gene            complement(10946..11965)
                     /locus_tag="KPH29_001761"
     CDS             complement(10946..11965)
                     /locus_tag="KPH29_001761"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBG1467361.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVAEKLDIDMPITNAIYSVINKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(11996..12610)
                     /gene="plsY"
                     /locus_tag="KPH29_001762"
     CDS             complement(11996..12610)
                     /gene="plsY"
                     /locus_tag="KPH29_001762"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBG1467362.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(12645..13964)
                     /gene="der"
                     /locus_tag="KPH29_001763"
     CDS             complement(12645..13964)
                     /gene="der"
                     /locus_tag="KPH29_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBG1467363.1"
                     /translation="MSRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRIFA
                     EVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAADK
                     EIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLDEI
                     VENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAIDTY
                     FEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTEQD
                     TKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAKTN
                     QRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTDIR
                     PPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(13998..15326)
                     /locus_tag="KPH29_001764"
     CDS             complement(13998..15326)
                     /locus_tag="KPH29_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HBG1467364.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEIGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADAHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPENILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(15564..15764)
                     /locus_tag="KPH29_001765"
     CDS             complement(15564..15764)
                     /locus_tag="KPH29_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467365.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(15766..16698)
                     /locus_tag="KPH29_001766"
     CDS             complement(15766..16698)
                     /locus_tag="KPH29_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBG1467366.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNM
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(16743..17675)
                     /locus_tag="KPH29_001767"
     CDS             complement(16743..17675)
                     /locus_tag="KPH29_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBG1467367.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYDLSFFHCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNDVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(17730..19019)
                     /locus_tag="KPH29_001768"
     CDS             complement(17730..19019)
                     /locus_tag="KPH29_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG1467368.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(19048..19740)
                     /locus_tag="KPH29_001769"
     CDS             complement(19048..19740)
                     /locus_tag="KPH29_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1467369.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(19755..20516)
                     /gene="pgeF"
                     /locus_tag="KPH29_001770"
     CDS             complement(19755..20516)
                     /gene="pgeF"
                     /locus_tag="KPH29_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HBG1467370.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENITSNSQIHSDIVNIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(20513..20974)
                     /gene="nrdR"
                     /locus_tag="KPH29_001771"
     CDS             complement(20513..20974)
                     /gene="nrdR"
                     /locus_tag="KPH29_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBG1467371.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIEEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(20996..21259)
                     /locus_tag="KPH29_001772"
     CDS             complement(20996..21259)
                     /locus_tag="KPH29_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBG1467372.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(21363..22136)
                     /gene="sigG"
                     /locus_tag="KPH29_001773"
     CDS             complement(21363..22136)
                     /gene="sigG"
                     /locus_tag="KPH29_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HBG1467373.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(22228..22950)
                     /gene="sigE"
                     /locus_tag="KPH29_001774"
     CDS             complement(22228..22950)
                     /gene="sigE"
                     /locus_tag="KPH29_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HBG1467374.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(22975..23847)
                     /locus_tag="KPH29_001775"
     CDS             complement(22975..23847)
                     /locus_tag="KPH29_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HBG1467375.1"
                     /translation="MFKGFKGGYMVYIEYYVIENLVINYIIISCTSILTKRYNPKKNK
                     LLGASLGSAYSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLV
                     NIFISGSTYFIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTINI
                     NLLEKSFCCKALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQ
                     EIIGSLDDKTSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDR
                     EYGAIMNPNILPGF"
     gene            complement(24138..25412)
                     /locus_tag="KPH29_001776"
     CDS             complement(24138..25412)
                     /locus_tag="KPH29_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBG1467376.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(25696..26853)
                     /gene="ftsZ"
                     /locus_tag="KPH29_001777"
     CDS             complement(25696..26853)
                     /gene="ftsZ"
                     /locus_tag="KPH29_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBG1467377.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(27043..27396)
                     /locus_tag="KPH29_001778"
     CDS             complement(27043..27396)
                     /locus_tag="KPH29_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HBG1467378.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(27495..28169)
                     /locus_tag="KPH29_001779"
     CDS             complement(27495..28169)
                     /locus_tag="KPH29_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBG1467379.1"
                     /translation="MKKKIVILGAFILGIFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKNNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VNLFAK"
     gene            complement(28174..28872)
                     /locus_tag="KPH29_001780"
     CDS             complement(28174..28872)
                     /locus_tag="KPH29_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBG1467380.1"
                     /translation="MKKRFINKSIIIGSVVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKTEKENLQSEVNSVKKDIETYRGKNNKGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVFNTYMY
                     LKEDKLCMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(28895..>29366)
                     /locus_tag="KPH29_001781"
     CDS             complement(28895..>29366)
                     /locus_tag="KPH29_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HBG1467381.1"
                     /translation="KLPNKIIISLKEKEIFAVLKDEDNYCYIDKKGNLLEELRGSNES
                     KKDLIVDVDYSVDDNKSIKFKNYKTKENVFKTLNYLKEEGIYRKINYVNLKKESNIEM
                     LTRSNIKILLSNDDNLDYNISRVSKILIDLQNKNTNGGTINLNYGKLAVYSPEG"
BASE COUNT         9079 a         5463 c         3044 g        11780 t
ORIGIN      
        1 ttaattaaat attattatat gtaatataac taatttttaa atgaatgtat aggagctgga
       61 atccttcctc ctctttgagt aaataattca gatgaatttg tacttatagg cattactggt
      121 gctcgtccta aaagtcctcc aaattctacc atatcaccta catcacatcc tggggctggt
      181 ataattctaa cggctgtagt cttattatta atcataccta ttgctgcctc atcagctatc
      241 atagctccaa gcgtacctgc tgtagtattt cctggcacag ctatcatatc aagacctaca
      301 gaacaaatag cagtcattgc ttcaagctta tctatactta aagcaccttt aataacagca
      361 tcaatcatac ctgcatcttc agatactggt ataaacgctc cactaagtcc tccaacatgt
      421 gaacatgcca ttactccacc ttttttaaca gcatcattta aaagtgctaa tgctgctgta
      481 gtcccatgag taccaacaac ttcaagaccc atttcctcta atatatttgc tactgaatca
      541 ccaatagcag gtgtaggagc tagtgataag tctattatcc caaatggaac atccaatcta
      601 ctagaagctt cctttgccac aagttgtcca actcttgtaa ttttaaatgc agtcttttta
      661 actgtttctg aaacaacatc aaaaggctct ccctttactt tctctaaagc tctcttaaca
      721 acaccagggc cacttacacc aacatttata attctatcag cctcaccaac accatggaaa
      781 gctcctgcca taaatggatt atcttctact gcattacaaa atactactaa ttttgcacaa
      841 ccaaaacctt ttgcatcttt tgttaattct gcagtttctt ttatgatttc tcccatttct
      901 ttcactgcat ccatatttat accacatctt gtagaaccta catttactga agcacatact
      961 ttattagtac ttgctagagc ttttggaatt gacttaatta gtatcttatc accttttgta
     1021 tatccctttt gaactaatgc tgagaatcca ccaataaaat ctatacctaa atcctctgca
     1081 gccttatcca atgtctgtgc aaactttaca tagtcatttt catctgttgc acctgcaata
     1141 atggaaattg gagttactga taccctttta tttacaattg ggataccaaa ttcacgctct
     1201 atatcttttc caaccttcac caaatcttta gcagaactca taatcttatt gtatattttt
     1261 tctctagctt tatctccatc agggtctata caatctaata aagaaatccc catagttata
     1321 gttcttatat ccaactgttc ttcatctatc atctttattg tttcaagtat atttcctaca
     1381 ttcactatta tcacctcccc ttactgtcta ctacagatta tgcattgaat taaatatttc
     1441 ttctcgctga atttttacat ccactgataa tttcttacct ctttcaacca agtcctcaac
     1501 cgttttctca aaagaattta atgatttctc taaatcaact atcataatca tagtaaaata
     1561 atcctgtaat attttttgtg atatatctac tatgttgata ttttgcttgt ataattcatc
     1621 actaattcct gccactatac ctggtttatc ctttccaatt actgttaaaa ttgctttcat
     1681 aataatatcc tcccttattt ttttataaat cttatatttt tacaaattga actatgtatt
     1741 aacatatatt tcatttattt gttagtaaac cactttttat gtatatattt aatatatttt
     1801 aatacctata cattcatttg tcaatttttt ttcaatattc cattattttt atactattta
     1861 gaaaattcat attttatata tgttttctca acaataaata cagttttcat aataatgaaa
     1921 ggtattactt agcaatctcg tgattttcta aataatacct ctctaaaaat tattttttta
     1981 attttatgaa tttaattata ctttttctag tatcaggatt tacaattaat atagaaccaa
     2041 tcataattgg tgttagtata caaaataaaa aaataatatc tttttttaga ttatattctt
     2101 tagattctaa tttataatta ttatttatta ttccatcata atcacttata tttgacttat
     2161 ttttatttgt atcttgattt ttgttagaaa tactttccct ataaggaaaa gaatgttcat
     2221 tggtaccaaa tactgatttt gagcttaaat cataattatc atcattttct gtaattggat
     2281 ttttattgta aaatcctttt tctttatcag aattattttc tttattaccg tctttaggta
     2341 tatcatgagg atttttattt tcattgctat cagtacttga ttctacatca tctttaggag
     2401 tatttggttg tttctcatca gaattattat tttcattatt attgttttga ttgttattac
     2461 tatcattatt ttcatttcca ttattcccat cttgattgtt atcatcctca tctctatccg
     2521 ggtcgtctct acctcctcct ccacggtcac catcaatatc atcattttcc cgttcatcat
     2581 ctggcgtctc ttggttgtct ccgcctccca ttataaatcc tggtttaaaa actatatttg
     2641 aatacccttc ttttaatcct cctaccactt ttactctaaa acaacgcaac ttaaaatctt
     2701 caaaaaaaag tatagcttga tctctaatat cctcctgttc ttcagatatc caattgattc
     2761 catcgcttga atattcaaca aaaaccttac ttgcactata cggcatatca taaaataaaa
     2821 cagctacata tccatttttt gtattttgaa attccagttc caaattgtct attgtttttt
     2881 tctctataac agatacattc aaaataggaa ctacatcatc atttattata attttttctt
     2941 tatttaacat ttctgtttta aactttcctg ttatatcaaa attttctccc ttttccaact
     3001 tttcataata atcagtaaaa tcccattcta catctatatc aagttgatta ccactagatg
     3061 tttctaatac catatatttg ggaaatttta tattttctat tttatcatct ttacttactt
     3121 ttatttcatc actaaataca ccaattcccc tatgacaaca tttcacaata ttatagccag
     3181 aatctttagc tatagagggt ataagctcat tataatcatc aattatataa actttcttac
     3241 tttctgatat aattgattgt gccaacttat tattatctct tagtaaccca ctttcattgt
     3301 tgctactata tgcttttatt gaagtttctt ctatctcaac atcaccttta gaataaacac
     3361 ctatagcatg ttctccattc acttcaatat ccttatttag tattctaata tcattttcag
     3421 tataaattcc tattgcatct tttccatttg cctttatttt ttcaacagat tcgtaggatg
     3481 atttaatatg taagtctcct ccttcttcaa cgtataaggc tgtcccatca gtagcaatta
     3541 tgttgtttat accaatactt atacttccac cattttctat atgtattact ccttcttttc
     3601 ctccttcacc tattatattc aattcattat catcaataat aaatctccca tgatttttta
     3661 tcaatatttt atatttgttg gtatctattg taaaattact gtcataagga atataaaatc
     3721 taaactcttc atctattacc atattacaag aaagagtata aactatttct tctttattat
     3781 ttttattttc ttgaacccac ctttcaaaat cttcaagaca attttcctct tgtccaaaaa
     3841 ctttatgtgg aaaacttata aaacacaaaa ctattatact cataaaaata aatatctcat
     3901 actttttcat cttacactcc tagaaatttt ttagctttga aacaatcctg tcgaaacata
     3961 tacttcaccc actataattt ttccacaatt atcaattatt gtcaatttat ataagtatct
     4021 ttattaattg ttataaataa aaagctactt actaaagtaa acagacgttt cactttaata
     4081 agtagctata tttttataac aaaatagtta taatattaga acttccttca tttacaattt
     4141 tttgtaaagt cttttgcatt ttaactctta tctcttctgg catagtatat aatttacttt
     4201 gtaattgctc ttttactaag tcatgtagtg acttaccaaa catatttgat tcccataaat
     4261 ctgctggttg ctcttcaaat acttccataa gatattttat catctcttca ccttgatttt
     4321 gatttccaac tattggagag acctctgtag atatatctgc ttttataata tgaagtgatg
     4381 gtgcattggc ttttaacttg ataccatatt gttttccttg tttcattatt tctggttctt
     4441 ccagactaag ttcttctaaa gaaggagcta ctactccata tccttttatc ttggcatcta
     4501 ttaaagcact ttctatttta tcatactcat ttttaacttt agatagtcta gttatcagac
     4561 taagcagttg ataatctcct tcaattttaa atccactttt ttcttcaagg acattataga
     4621 ataaatcttg ctttgtctgt aaatcaatat ttataacacc ttctcctagc tctacattat
     4681 caacacctgt atcttccaaa aactctaact cagaaaatcc ttgtataata ccttctatat
     4741 ctcttatttt acctatatct ataatacttt gttttaatgt tgttattatg ctacttttta
     4801 tccaatgatt tctttcaaga ccttcaaccc attttggtaa gttgattctt atttctgtaa
     4861 gtggaaaatc atataataca gcttccataa cctcctctat atcttcttct tccatttcaa
     4921 ccacattcat tggaagaact ggaacttcat acttttcttc caattcactt ctaagcatac
     4981 tcgtttcctc actttttgga cttaacgtat ttaaaaccac tgcaaatggt tttttaagat
     5041 ttttcaattc ttgtataact ctctcttctg gttctacata gctttttctg tcaattccag
     5101 taactgaacc atctgtcaag acaactatcc ctattgtaga atggtctttt ataacttttt
     5161 tagttcctat ctctgctgcc ttttcaaatg tcatagcttc ttgtgaccaa ggagtggata
     5221 ctaatctttg ttttccacct tcctcatgcc ctaaagctcc ttcaactatg tatccaacac
     5281 aatctaccat tcttactttt aaagatactg tgtcttttat ttttatttct acaccatctg
     5341 ctggtacaaa ttttggttct actgtcatta tcgttttacc tgaaccactt tggggtatct
     5401 catctcttgt tctatccttt ttaaattcat tgtctatatt aggtataacc aacttttcca
     5461 taaattttct tataaaagtt gattttcctg ttcttacagg tccaactact ccaatatata
     5521 tatccccctg agtccttttg gatatatctt cgtatatgtt attattcata ttattcctcc
     5581 tatacatcta atcttattaa attatattta gagttaaggg ttaatattct taaaatagaa
     5641 aaataaagac cagcaatatt aatttaattt acatatagaa atttgtaaat ttcaatgtaa
     5701 attaaattaa tattgctggt ccaaatagta gtgtattata ttaactgact ttagtagtat
     5761 tttcatttga cttattattc atctcaataa gttctttacg tctttctttt gtcatgttca
     5821 tccatataaa tgtaaacacc aatccaatta tggctactat agtaagaact atgaagtagt
     5881 acctatatcc agttacaccc ggaaatgcat ccaatatctt ccctgctgct aaaggacaaa
     5941 gtacttctgg aagatatcct aatgtagcaa ctattcctat agcagttcct gatattgata
     6001 atgaataatt accttcttca agtaacgaat agtgtaaacc ttgaattgca tacatagata
     6061 catatattgc aacacatcca atcaatagta tataaatcat agatgatttt gttggtgtaa
     6121 atattacacc taaaagacct gcaatcataa gtataaatcc ataagatata atctttgatg
     6181 aacctacttt atctccaagt aatccagaag cagctgatgc tactggtcta caatactgag
     6241 ctaatattga gattgcagat gcaaacacca cagtagctcc aaatgcttca gttgaataag
     6301 gcgtaaaata ataaaatgac atattagctg aatatgaaca aaacattatt attgttatta
     6361 tccatgtatg aggcattttt attactgcaa aaaaggcttc tttatcaaat tttgctgata
     6421 cctctttttc attatccttt aatttgaaaa atactaatac tccaactatt acatttattg
     6481 ccgagtataa tactataata gctgtaagac cagatttatc acttactttt gatgaaatat
     6541 acccaaatag tatcaatact agagatagat gaattgcatt ggtaattcca cgaccacttt
     6601 ccataaatcc aaaagctttt ccttgttcat tttcagatgc taacattcta acagctttta
     6661 caagagctgg ccaaaaagta agtattgttg ttattcccca taaggcatgg attaaaaaca
     6721 caacaatata tggaggataa agtgttaata caagaccaga tattcctgta ataattaaag
     6781 aacctgaaag caattttctg gctgaaatac ggtctgctaa aaaaccacca aaaaggtatg
     6841 ataccattgc aagtagacca taaatgcttc ctaagcttcc catttgtgta tttgtaagat
     6901 taaatgcttg tgcaaaagta tcataataat aattctttaa atatggtaaa gcatatataa
     6961 atgctcctgc tacaaagagt attatcaaga ctttgaaatt tttccaaatg cttggtttat
     7021 ttacttcaac tgacatactt cctccttatg taaatacctg taaattttca tgataattaa
     7081 ttattgctta ttttattgtt tattcaatat ttcggaatat ttattaatat attaggttaa
     7141 tctatcaata atttatggtg agttatgagc taaaagtaaa ctcttttagc tcaccataat
     7201 aattatattt ttatttatac taacgtccag agttattaga tatctttttt tcatagttct
     7261 tccatagcat taaacctgtt attataacaa aaagacctcc tcctatcatt tcaatctcat
     7321 agattatatt atcttttaaa gtctccataa ctggtgccgt tgagaaaagt atgccaacta
     7381 cagttactac aagtaccatt ctggcaatat tgatacaaac tgaatcactt tttgcaattg
     7441 aatatggtct ttcttcattt ggtcttgtct ttctaagtct aatataagat gtaaaaagca
     7501 agacatatgg aaataatgct gttaatgctg tcatagtaac aagaacattg tatatattat
     7561 ctactgatgg taataagttt gttcctacaa gaagtagact cactattata gcctgaacta
     7621 ttactgcatt tgatggtata ttgtatttat ttgtaacagt caattgctta ggaaatacac
     7681 cttctcttac atttccaaaa agcatcttta ctggacctgc tatataaagt attaatgctc
     7741 ctagtactga taaagttatt ccaaatgcta ctattcttat aaaccaactt cctataccta
     7801 aatcttgtgc aactttagct aatgcatcta gtatccctgt agacgctgca attttatctg
     7861 gaggaagtat cattgttatt gctactgaac ctaagatata gattcctcct attaaaacag
     7921 ccacagttaa tatagcttta ggaaagtttt tcttagcatt atccatttcg gttatgaaat
     7981 tagcagtcgt ttcagctcct gataatgcaa atattatagc agatatagaa actaatgaat
     8041 tggcatctat ttttggtatt atattttgga ttgtatatat cgatgctgat ggagcttttt
     8101 tgagaataac tacagacata aatgccatca ctataagtaa tatagcagga attgtagaac
     8161 ctaatgctcc tgtattcgta aaaaattttc caaatgccat cccacgagta cttactaagg
     8221 acaacagcca aaatattatg agtgaaagtg ctaaaataaa catcttattg tttgataaat
     8281 ctggatttcc aagcatatat gcaatattta tagctaaaaa tgtcaagaaa gaagaatacc
     8341 aaaatatctt tgatgtccaa tttagccatg acaccataaa tccccacttc tctccatagg
     8401 cttgtttaac ccattctcca agtccaccat cttttggata tgttgcagcc aactctgcac
     8461 atataagtga tgcaggaaca aagaatataa aagcaaacaa taaccaaact ggtatagaac
     8521 caagacccaa tccaaaacta gaggcagtag atttagctat ccatctaatc ccaaatagtg
     8581 ctgatatatt cataaatact aaatcccaaa atccaatttt ctttttcact gtaattcctc
     8641 caatttcaaa tttattcaaa ctcatacaca atgtattgat ttaaatatca agtataagtt
     8701 cttcaacact tcttggataa ttagttataa ttgtagcacc attcttacct actattgctg
     8761 tatctgagtg tctaaatcct ccaactccag gtatatatat ccctggctcc atagaaaata
     8821 ccattccttc ttctaatatt aattcattgt caaatcttaa atatggctct tcatgttctg
     8881 ataaacctaa accatgacca attctatgat ttgaatataa ctctagacca tactttgcaa
     8941 caacagctct tgctgcttca tccaccattc ttgctggtat accagctttt attgtatcta
     9001 atccagcttg ttgagcctct actgcaatct taaacacttc tttttgtctt tctgttggtt
     9061 tacctataat aaaagttctt tcattttctg ctctatagtt ttcataccaa acctgtctac
     9121 tatgtattac tatatctcct ctttgaagta tcctagtatt ggaatctaaa tgaggttgtg
     9181 ctgttctatc aatccctgaa caagtccagt ttgcaaatcc tatatatgta tcaggataat
     9241 tttcagaaac atattttagt aaagcattat cccccgcaac gtcaaactcc aattcactca
     9301 tcccaactct aacattttct aaagaccctt ttattcctat atcagatagg taacctgcta
     9361 tcttcaaaaa ttctatttct tttgcatctt ttacatatct catttcaaat attttacttc
     9421 caacatcttt gatagcaaat gacttatcag ttatatactc tgaaaaactt gcagatacta
     9481 tatctttttc tattccaaca gttgttcctt tagggtattt atttaaaatt atatctaagt
     9541 aatgtttatg tgatattcca tgttctttca tttctggtat ttcataatac acatatacat
     9601 tatcaacttt tgcaacttct ttagcatgta attcttctaa tccaggtact attaattcta
     9661 ttttatcttg agttactact gttacaattg gtctagaata agttattgct ttaaatccac
     9721 taatataaaa ttgattgtct ggttcaaata ttaaagcaat ttcaatgctt ttttcattca
     9781 tgtactttct caatctttct agtctcatat tatttgacat aatgcacctc tttcttaata
     9841 ttcagataat ttattcaata caacaaggat aaatatatta ttttaaaatt cttaatattt
     9901 ttagtacttg tcgtatttgc atgtaaatta attttatttg tgattatttt atcataatca
     9961 tgattatttt gtcaatcacg attttatttt taaaattttt ttatattcta gtgattttaa
    10021 attatctaaa atataacgag tatacattgt attttggaat attattcttg atttatggta
    10081 agtttaatga taaaatcacg attatataaa ctatattgat tatttgtagg tgaataaaat
    10141 gacaaataaa gaattacaaa agaaaaggat attgatgtat ttcatagaag ctgctcaaaa
    10201 tattatggaa aacgaaggta tagaaaatat tacacttcgt aaggttgcag atatggctgg
    10261 atataatagt tcaactctat ataaatactt taaaaattta gaccatttaa tatcatttgc
    10321 atctataaaa tattttaaag attataactt gaatatttca agatgtatag aaaatgtcaa
    10381 tgatgaatat aagaaatata taattatgtg gaaattattt tgtaagcatt cttttgacaa
    10441 tgctcaagct ttttatcagg tattttttaa cttgtctagt gatgaactta gctatataac
    10501 aaaacaatat tatgatatgt ttccagaaga tttaggtatt catgatagtg acatatcttt
    10561 aatgattaca ggagaaagta taaaggaaag aaataagata ctattaaaca atcttgtaga
    10621 tactggatat ataccagcta aagatttaga cataatcaat gatataatta tttcttttta
    10681 tcaaaatcta ttattcttaa aaaagaacac aggaaaaaat tctggtgatg atgaattaac
    10741 tatgaaaatg atttcttcta ttgaatacat aataaaacat gctaaataaa attaaaatat
    10801 ggctgtctca aaatagagat aagccttttt tattgttgaa ataataaaaa tatatttaat
    10861 ttcatataaa aaaaagtgcc tcgtaaacta aaaagttcat tttgagacac ccctaaaaca
    10921 aatttattat ttattataca atagtttata tatcatcacc taaaactact tcttccattt
    10981 catgcttttt acttctcatc attagttcta ttccaacttc tttaggattt gaacctttat
    11041 ttattacaga atatatagca ttagttatag gcatatctat atctaatttt tcagcaacat
    11101 catgtgctac ttcagtagca gttatgcctt ctacaaccat ctttacttct tttaaagtat
    11161 cttctaaact cataccttta cctatgagta taccagctct tctatttcta ctatgcatac
    11221 ttgtacaagt aactattaaa tctccaatac ctgaaagacc tgcaaaggta gacatattag
    11281 ctcccatagc aattccaagt ctgctcattt cactaatacc tcttgtcata agggcagctt
    11341 ttgcattatc accataccct aatccgtcac atatcccagc tccaaatgct ataatatttt
    11401 ttaatgctcc tccaagttct actccaacta tgtctggatt cgtataaact ctaagtttag
    11461 ggctcataaa taaatcttgt atcatctgtg ctatttttaa atctttagaa gcaactacta
    11521 cggtagtagg aatatccctt gctacttctt cagcatgaga tggcccagat agtattacat
    11581 atggattttg tggtaattct tcttcacaga cttgagataa tctaagccca gtcccttttt
    11641 caagaccttt agctacatta acaataactt gtccttcttt tataaatggt tttatttttt
    11701 ggcaagtact tcttacagcc tgtgatggaa ctgctaaaac tattacctta caatctttta
    11761 tagttttttc tatatcagta cttatagtta tattgtttgg aaataatact cctggtaaat
    11821 agtctgtatt ttctttagtt ctgtttattt ttttagcttg ttcctctttt cgagtccaca
    11881 tgctcacatc attacctttt tttgcaagcc ctaaagctaa tgcacttccc cagcttccag
    11941 tacctaatac acatactttc tccatgttta acaccacctg agtataatat atttactatt
    12001 ctttcttttg accaattttt ctctctgtcc catttaataa tcgctttata tttgctctgt
    12061 gattatatat tactgatgca gttaagaaca gagctactat taaaccagct ttatttttta
    12121 ccataatcat aaatattggt gaacatccta ttcctacaac agaacccaat gaaacatact
    12181 ttgttatagc taccaccaat ataaatactg ctagacacat tagtgttatt actggattta
    12241 cagctagcat tgcacctagt gaagttgcaa cacctttacc tcctctaaat cctaaaactg
    12301 ctggccaatt atgacctgct acaacacata taactgccag atatccagct aaaagagtat
    12361 ctattccaac caatcttgct gcaaattctg atattaaaac agccattaca cctttaagaa
    12421 catcacctaa aaatgtcatt gctcctgctc tttttccaag tgtcctaagt acatttgtcg
    12481 aacctgcatt accagaacct tgagtcctta tatcaacccc agcaattcgc tttgcaacta
    12541 tgtatgaggt agatatattt cctaaaagat atgcaactac agcaataatt atataactaa
    12601 atatctccac tgaatttccc ccctttagtt atattttaag atatttattt tttatttttt
    12661 tgtctatact caaactttat ggaagttcct tcaaatccaa aattttctct tatcttattc
    12721 tcaaggtatc tttgatatga gaagtgagtt aaatccttat catttataaa caatgtaatt
    12781 ttaggaggtc taatgtcagt ttgagtccca tagtaaattt ttagtcttct acctttgtca
    12841 gatggtggtt gatttaacat tacagcttct ccaattactt cattcagtgc tgaagttgat
    12901 attctcttag aatgttcatt tgacacatat tcaactgtat ctagtatctt gttcattctt
    12961 tgattagttt ttgcagaaac aaatacgatt ggagcataca tcataaatgg aaatttttct
    13021 tttatatctt ttgtgtaatt actcatagtc ttattatctt tctctattaa atcccattta
    13081 ttgattacaa atatgcatcc ttttccttca tcatgtgcta tacctgcaac ctttgtatct
    13141 tgttctgtta caccttctaa ggcatctatt actatcaata ctacatctgc tctatctaca
    13201 gcactcatag ctcttattac actgtatttt tctattgtct catatatttt actttttctt
    13261 ctaagccctg ctgtatctat tagaagaaat ttctgtccat ttttttcaaa ataagtatca
    13321 attgcgtctc tagtagttcc tgcaattgga cttacaatta ctctttcttc tccaagtatc
    13381 ttattgagta tagaactttt tcctgcattt ggtttacctg ttatagctac tcttattata
    13441 tcttcttcat actctgtatc taatcctgct ggaaaatttt caactatctc atctaataaa
    13501 tctccaaatc ccatactatt ggctcctgaa actgcaaaag gcgttccaaa tcccaactca
    13561 taaaaatcat atatattatc aaattgactt tgactatcaa tcttattaac cactaagatt
    13621 actggttttt ttgtctttct aagcaattgt gcaatttctt tatcagcagc tgttattcca
    13681 gctttaccat caactataaa aagtattaca tgtgacatgt ccattgcaag catggcttga
    13741 tttctcattt gagataatat tatatcttcg ctatcaggtt caattcctcc tgtatccact
    13801 aatgtaaaat atttatctaa ccactctact tcagcaaata ttctatctct tgtaactcca
    13861 ggtgtatttt caactattga tatccttttt cctgcaaatt tattaaatat tgtagactta
    13921 ccaacgtttg gtctcccaac tacagctaca actggcctac ttatactcat tttatttctc
    13981 cttccatctt ttgttttcta ttttaaaatt ttatccacaa agtcttttcc ttcattcaaa
    14041 caagggataa tttttattct cataagttct tctaattgat ttaattcaat attatctaag
    14101 aaaatttctt catctgcttt tagcatactt cttgttatat aaagagcttc tcctaattct
    14161 ttatcttcaa gttgttcttt taaatcctta gctgttaaaa gacctgatac agttattgtc
    14221 tctccaaaaa acttgttttt gatttcatat acatttactt gaagattttt aaacttgtcc
    14281 atcatagcat ctgccatact ctgtataaat tcatatgcag aatgaccagt agctattgat
    14341 actttcttct ctctttttag aatattctca ggcaatatat ttagatactc ttcaatctct
    14401 gtttttaact tacgtatcat acctacacca tcttcaaact gtaaaaaacc ttcatattct
    14461 tcatatccag gcaattcact attagatagt atataaaatt catctgataa aaatgcaaat
    14521 cgagtaccta atttttcaag atatttctgt tgtatttgat gtatttgctc tatagtttta
    14581 cttgcgctct tatcattaaa tatatttaat tctactagat tttctctatg ctttgtaatc
    14641 ccaactggaa ctatcgcaac actgtttaca tatggataaa gttgagctag ttcttttatt
    14701 gttctatcca attcctttcc atcatttata cctggacata aaacaatttg acagttcatt
    14761 tctatatgag catctgccaa tctcttcatt ataccatata attttcctgc aaatttattg
    14821 cttatcattt tttgtcttaa ctctggatta gttgtatgca cagatatatt tataggactt
    14881 attctatatt ttataatatt atttatatct tcttcactca tatttgtaag tgtgacaaaa
    14941 tttccttgta aaaaagataa tctcgaatca tcatctttaa aataaagagt ttctctcata
    15001 ccttctggca attggtctat aaaacaaaac atacacttat ttctacagct tttagcttgg
    15061 tcaattatcg gatttgtaaa ttcaacaccc aaatcttcat catagtcttt ttctatctcg
    15121 tatatgtaga cttcgccatc ttttttttgt atttctacat ctaaatattc atcacttagt
    15181 aagaatctat actctattat atcatgtatt ggctgttcat tcacagatat aagtaaatct
    15241 cctacctcta tacctatttc ttctgcaata ctatctttat aaaccttact tataatatta
    15301 tttacttttt ttacatttac ttccaatatt ttcaccactc ttttgtattc atcaatgctt
    15361 attataatat attttaatat gcaatttatt tatttattat attttatgat agcattttta
    15421 agcatttcat gtcataataa cacattttaa ttctaatagt caatataagt aaatagctat
    15481 aaaatagatt agaacaaata atattcaaat ttaagtttct taaaaatttg aatattattt
    15541 gttcttaaaa aaattttata aatttaatgt tttactatga ttaacgtccc atttgtcata
    15601 tcatgaacca ttccacctac acactttgac atcaacaatg ccttattttg taagtctttc
    15661 tcatcatttg cataaaatat tggacaacct ccaccatttt tcatattctt atcaagtgta
    15721 actatagcta gggtatattc atttaaacct atatccattc ccatcttatg cacttccttt
    15781 ctttcctatt ttataatttt ttagtgataa gttttttcct ttagagcttg aaagtattgg
    15841 gcatgacttt acagcttcca ttactttttc catattctta tctactggta aataagcaat
    15901 cattatactt tcatcctctt tatttcttct aggtaatggt acgaatgaag gttctccata
    15961 atctccatat aaacctaacc ttgaatatat attgtatact attgcttgtc tttgaccgga
    16021 gtcaaataat attcctgcat tagcataatc cttattttta ggaattattt gtattcctac
    16081 acctctatta atatatactt ctctatcact ttttagacct atatttgtta taccttttaa
    16141 atctcccacc tttagtatag attcgtccac aaaactaatt tcagcaggca ttacatcagc
    16201 tatgtctcca atactttttc tagtaagaag tttttttaat ataaaggcta acactaatcc
    16261 acaaattgaa cttattacca aactcatccc ttcacttatt ttcgtttctg caagtattac
    16321 ataatacaat cctattgtta aaaaagatgt aactatacac atataatttc ttacttcata
    16381 ggttctagct atctcttcta tgaatgcagc acctctcttt acaagttgaa cttcctctaa
    16441 attttgcaat gtatttcttc tatttgctct cacttgtcta aattgttctg ctgctagaga
    16501 taaaaatgtt atggatgtat aagaacgatt tataagagct ggaaccaata atgctcctaa
    16561 tgaagaagcg acgaatgcta atataagttg agatagtaaa acatttggtt gcgttggata
    16621 ctgtttttgg tctaaattca acattataat tctagatgtt ataccaataa taacagcaac
    16681 aaaaaatact ctatccatta aatcactcct aaaatattta atttgaaatt ttacatgtaa
    16741 atctacaaag tctttctaga aaaatcactc tgctttcctt ttgatgtttc tatgataggt
    16801 gtacttttca ctacatctat catgacatct atatctttga gtattggcat atatggtata
    16861 accacagtac ttttttctaa attggtctta gatatagcaa caatatcaac ctcatctaca
    16921 tctctatcta tccccatatg aataaataga ttatgtatta tagcttgtct ttgccctata
    16981 tcatttacta ttccaaatgc acctaaatct cttggtataa cttctatggc cagacattca
    17041 tttctatact tttctcttgt actttctaga cctatattag ttattattac atcatttacc
    17101 attaaaatag gtccttcaaa acttattttt gcaggaacta tatctgctat atctgctata
    17161 ctgtttcttc ttaaaaatct tctaaaaatc aagccaacaa tagctcctga cacaatagct
    17221 aaaattgtgc aatgaaaaaa gctaaggtca tactttcttg caaaaacgat ataaactatc
    17281 gaagatacta gtgcagaaaa aagacttata taacttctag attcatacgt agatgctatt
    17341 tcttcaacat aagcatctcc ttttggtact aattcttcat tatcaatatt ctttagtgtt
    17401 attctttctt gttccgcaag accttgaaac tgttgtattg ctactgcaaa aaatgtcaag
    17461 gctgcaaact ccttatctat aagtgcaggt aatgctatag cacctaacga agctgaaagc
    17521 ccagatataa ttatctgttc taaataatct tgtggtcttg atgggtattg tttgtctgta
    17581 actcttaaaa ctaatcctct acataatatt cctacaacta atgcaactat aaaagagtgt
    17641 ctaaataaat ttgcattcaa taaactttct tccattttct cagctccttc tcttatatag
    17701 tttgtgcaaa aaacaaaaaa aattcccttc tatttaagga aatttttttg taatattgta
    17761 actataaatt cgctaccaat gccaagttca ctttttactt ttatacatcc atttagtgat
    17821 ttgactatat gctttgttat agccaatcct aaacccgttc ctcctacatc cctacttctt
    17881 gctttatcta ccctataaaa tcgttcaaat attcgttcta catcttcttt tggtatccct
    17941 acaccattgt ctataacttt aattacgatt ttttcttcat cttttaacac tacaacttgt
    18001 acaatcccat tatcttgggt atactttata gcattatcaa ctaggtttaa gaacacttgc
    18061 tttatatagt ctttattgga tagtatactt attgatttat catcacattt atataaaact
    18121 tctatatttt tttcactagc tgaatgttga gttatttcat atacctcttt aaatacatca
    18181 tatactacaa cattttccat aagaagagta tctttatttt ctacaaatga taacagcaat
    18241 atatcatcaa tcaacctttt taatctatct gattcgcttt ctattatttc taagaatcta
    18301 tttctagtat tcacatctat attttcattt gactttagtg tttcaacaaa cccacttata
    18361 gatgtaagtg gtgttttaag ttcatgactt acatttgcaa caaaatcact tctcatattt
    18421 tcaagtttaa cttttttagt aatatcttct atatttacta tcgaacctat tattacgttg
    18481 ttagtattct gaagataaac tgggtctacc tttatcttat atacgatatc atctttagta
    18541 gtaatattag cagtttcatt atctttagaa ccaataaatt gaagtatttg ttttaatatt
    18601 tcttcttctt ttattacaac attaatatta tgaccttcaa ctaattgatt tggttcactc
    18661 tttatcattt cttttgcttc atcatttatc agaagtatat ttccatttac atctatagcc
    18721 aaaattccat gagatatact ctttaatata gatcttaact gtaaatgttt atactgtact
    18781 tcttctaagg ttttatccat attctttatc atatgattaa aatttctagc taattcacca
    18841 agttctcctt tggctgatat gttaagccta gcatgatatt cattattact tacttttttt
    18901 gagacgtata taaactcttt taaatatttc cttaatgtta ttgtatatct tacagctaat
    18961 ataacaatgg ctatggacaa cataacaata aaaaaatata tactatttaa atcttccaca
    19021 agataacact cctaaaatat aaccttttta atctatttta taccctacac ctcttatagt
    19081 ctctatatat ttttccgatt tatcttcatc ttctattttt tttcttaaat atcttatatg
    19141 aacatctact gttctagttt ctccatagta ttcatacccc catattttat ccaaaagata
    19201 atttcttgaa agtacttttc ctttattttg aattaataat tttaacaatt caaattcctt
    19261 aagagttaac tctatctttt cagagccttt ggtaacttca tgttttgata aatctagttt
    19321 taaattgcct gtagtaagta tattgttttc ttcacctaag ctagtcaaat tatatctcct
    19381 taaaacagaa ctaatcctag ctaacaactc ttttatacta aatggcttag ttatatagtc
    19441 atcagcacct atttcaagcc cttcaacctt gtcattttcc atatttttag cagtaagcat
    19501 tataacagga atatttttta agtccttatc acttcttatt tttctcaaaa cttctattcc
    19561 acttatattt ggtaacatcc aatctaaaag tattaaatct ggtttaattt ccttagcctt
    19621 tataaatccg tcaaatccat catatgaata actaacttcg taatttgaca cctccaaatt
    19681 gaattttaat agctcaacta tatgcatttc atcgtctata acaagcactt tagtattcat
    19741 taaaaagctc ctctctattt tatctgtagt accgttccta ttcgttctga tgccttatta
    19801 tgttttctat aagagtggaa tttatctgct ctacaactag tgcataaatt taaatttatt
    19861 atattttctt ttttaactcc actacatcta agcatatact cattaatttt ccataaatct
    19921 aaatagtaac ttccctcttt tattatatag aacttttcat cattatttgt aagaattgtg
    19981 ttaaattttt ctactaaatc ttcagacact tcatagcaac atggtccaat tgatggccca
    20041 attatacata ctaaatcttc aggaactgtt ttatagcatt cagacattaa ttttattgtt
    20101 ttatgaccta tattactaaa agtccctctc caacctgcat gagccagtcc tatggcttta
    20161 tttttagcat ctataattga tataggtaca cagtctgctg taaacagtaa aagtggtact
    20221 ttttccaagt ttgttataag tgcatctcca tctcttcttt tccctatagt atctttattg
    20281 actatattaa ctatatctga atgtatttga gaattactag ttatattttc aaatgtcaaa
    20341 tcacattctt tacatacttt taacatatct tctctatttt ttgcatctat attcttacat
    20401 gttataacta gatttacaga atttaatttt tcatcaagtt cttcacaatt accacttaaa
    20461 ttaacatact ctttaatatc ttgtacattt tttatattta tataatcttt catcatttgt
    20521 ctcctttttc tatcagtata cttttaagtt catttacaaa agtatttata tctttaaatt
    20581 gtctatacac agaagcaaat cttacatagg caacctcatc taactctttc aatttatcca
    20641 ttaccatctc accgattttt ttggtttcaa tttcttctat aaaacattta tttatttcat
    20701 tttcaatatg tacaacaata ctttcaatct cttcaataga aacaggtctt ttttcacatg
    20761 cttttagcaa tccatatttt atcttttctc tatcaaaata ttctctacta ttatcctttt
    20821 taatgaccat taaaggagtt gtttctattt tctcatacgt agtaaacctc tttttacatg
    20881 attcacactc acgtcttctt ctgatagatt ttaaatctgt atgtctagaa tctattacct
    20941 ttgattcttt ataattacaa taaggacatt gcatctatat tcctcctttg tttaactata
    21001 tctcaaactc atctaagctt aattctgaac ctatatttac tattattgta tcacaaccta
    21061 ttttaagtat atcattccaa aatataactt cttcatcccc tcctcttgaa aaaaaacttc
    21121 tacttccatc tccaaaaatg gtaaatgaga ctaccttacc ttcgtgaata tccatgtcta
    21181 tgtctattat aaaccccatc ctttttccat tttttacatt tataatttct ttttccatta
    21241 tatcagaaac tctaatcata ttgtactcac cctttcttta aaaataaata tgtatcgaag
    21301 taaaaaaaaa tgacctatat aggtcattta aattaaaata atgtttacag tccatggatt
    21361 acttatacat attttctcat attttttaaa gcattttttt caattcttga tacttgtgct
    21421 tgagatatac cgatttcatc agcaacttct atctgagttc ttcctttgta aaaccttaaa
    21481 tctaatacca acttttctct actatttaat ttcttaatag cttctttaag tgatatttct
    21541 tgcaaccaat tttcatcagt atctttttta tcttgaacct gatccataac aaatattgca
    21601 tctccattgt cttgatatac tgggtcaaac agagatattg ggtcttgtat ggcatcaagt
    21661 gccattacaa ctgattctac ttctaattct aattcctttg ctatttctga tactgtaggt
    21721 tctttagaat ttgttctaat taatctttct ctaacttgta gtgccttata tgctatatct
    21781 tttaatgacc tacttactct aattgggtta ttgtctctta gatatcttct aatttctcct
    21841 ataatcatag gaacagcata agttgaaaac cttacatttt ggcttaaatc aaagttatct
    21901 atagctttta taagaccaat acaacctatt tgaaataaat cgtctatatt ttctcctctg
    21961 ttgttaaact tttttattac actcaagact aatcttaaat ttcctcttac aaactgttgt
    22021 ctggcttctt catcaccatt ttttatttgt aataaaagtt ccttcatttg cttattttta
    22081 agaactggaa gttctgatgt attaacacca catatttcaa ccttattaac ttgcatatat
    22141 tttcagtcct ctctcaaaag tattcatcta ttgaaattat ttacatttcc aattttttta
    22201 tactttttaa gaagaattaa tttaacatta tacaaatttt ttcatttctt tttgcaacct
    22261 tgagattatt ttcttttcaa gtctagatat gtaggactgt gatattccaa gcattccagc
    22321 aacttctttt tgtgtttttt caataccttt atcaataaga ccaaatctta attccataat
    22381 ttgcttttct ctgtcactta atctgtcaag tgccataacc aacaaatctc tatctatttc
    22441 ttcttcaatt attttgtata tttcatcatt ttctgtaccc aatacatcag ataataaaag
    22501 ttcatttcca tctaaatcta tatttaaagg ttcatcaaat gatacttctg ttttcttttt
    22561 attatttttt cttaagtaca ttaatatttc attttctata catctcgaag cataagttgc
    22621 caattttata tttttattca gtttaaaagt attaactgct tttatcaaac ctatagttcc
    22681 tattgaaatc aaatcttcta catctatacc tgtattttca aattttctcg atatgtagac
    22741 tactaatcta agatttcttt ctattaaaat tgactttaca ctttcatctg tttctagctt
    22801 ttgtaaaagc tccatttctt cttctggttt caatggagga ggaagtatat ttgcccctcc
    22861 catatagtat attccctttg gctttattat ttttaattta atacctaaca tagtaataaa
    22921 gcttattatt ctttctttca atcgtaacat tccttgtccc ccttaaattt tcacctaaaa
    22981 tccaggcaat atatttggat tcattattgc tccatattct ctatcattaa aatttgaaat
    23041 accaagtatt atatttccta tcttttgctc atctacttct atatagtcag cttttaagcc
    23101 tattattatg cttgtcttat tacttccagc atgtttataa ggtattattc taactctacc
    23161 tgaagtttta tcatctaaac ttcctataat ttcttgtgct ttcttcatat ctatatcttc
    23221 ataattaata tttaatagtt tttctggaat aaatccttct aacacactag acttaactat
    23281 tattacatca ctttgactta ttgggtcttt taacaaatta ccactatcta ctaaagcttt
    23341 acagcaaaaa cttttttcta gtaagtttat atttatagtc tttgtaagct ccttaatgta
    23401 cttaagcatt ttcaaatctt tataaatgta ttttaataat tcacaactca cataagtaca
    23461 tgcaattaag aaagatattt ttaagtgact tattcctgta aaataaatta taaaataagt
    23521 gcttccagaa ataaaaatgt ttactagata aaaaactaac gctacatgaa cataatcttt
    23581 tttgctcttg cttctaaatg aaattaaagt tattatgctc atgattatta ttttaaatgg
    23641 tacagtaaat agtatattca ttgatggata caaataagca acagaatagg cacttcctaa
    23701 actagccccc aataacttat tttttttagg attatatctt ttagtaagaa tagaagtaca
    23761 acttataata atataattta ttactaggtt ttctataaca tagtactcaa tatacaccat
    23821 gtatcccccc ttaaaacctt taaacatatt ataagatagt atgtacaaaa ttttatgtca
    23881 cttttgatgg tcgaatccaa aacaaattct atttaaaaag aacaatgata tatgtcttat
    23941 actttatgtc gtcaaaagat atttttttaa tgccaaacag atagactttt ttgtagaatc
    24001 caatatatct aggtagaagt tattcgcttt acttttggta tatttataaa aaaaagacta
    24061 cataatttaa taatatatca agtaaatctt ttacttatat aaaatcaaat tatatagtct
    24121 gtaaattcaa atataaatta ttgtgctagt aatccatttg catattttgc agattcattt
    24181 agtgcttgtt ctggagttat gtctccagct atcatcattt gaagttgttt ccataaatat
    24241 tcatatatct ctactccatg aggtccaact gtcaatggtc tatacatatg tttatctctt
    24301 gtcaccattt cttcaaatct tgggtctcct tggtattttt cacctttagc aactggagct
    24361 ctaggtattt ctttgtggaa agcttccatt gattctttgc ttaacatata ctcaagaagt
    24421 ttaaatccaa gttctttatt tttacatgct gacattaatg ttaaatcatc tactgaagca
    24481 aaacttccaa ctgtcttatc ttctaatgat gttacatatc cccagtttaa atccgggaat
    24541 gatttatcaa atgtacttgt agcagcagag gatagcatta tagtaaatgc agcttttcct
    24601 ggtccaaata cactttcaat catttcatta ctatcttttg ataaagcatc ttctggaata
    24661 tactttttca tatcatttat aaagtttgca gctttcaagc cttctgcatt attaaattta
    24721 actgatttta aatcatcatt atatatatct ccaccagctt gccataaata cggataccag
    24781 ttccagttta agcttccaaa tactttagaa ccccaacctt gtgcaagacc ccactggtcg
    24841 atttttccat ctccatcagt atcttttgtt gctttttcac agattctttt aaaatcatcc
    24901 catgtttttg gaggtttttc tcctaatttt tctagtatat ctttattgta gtacaatacg
    24961 cctggattag cagcttctat aggtaaacca taaataccac ccatcatttt tgcatcatct
    25021 aagtatagat aattatctga agttcctgat ttttctaaat aaggtgttaa atcttctact
    25081 gcacccattt ctataaattg agggaacatc tctgcataca tgtatcctat atctggacct
    25141 tcacctgcac ttattgctgt tgcatatttt tctgcgtagt tatcccatgg tactatctga
    25201 aattcaattt cacagttatt ttctttttca aatttttcaa atatcggctt ccatgcatct
    25261 gcatcatggt ctgttaatgg tgcagaccat acaactaatt tttctttacc ctctttgcct
    25321 ttagaatctt ctttttttga acatcctgca agaattgata ctgatagaat ccctaccata
    25381 actaaagaaa gaaccttttt tagtttaatc atgcttaatc ccctttcaga ctttaatttg
    25441 tctttttcct caaatgttta catttaattc agatttaata ttaacaaata agactactct
    25501 tgtctaatca taaaaaacta tactaactcc tataactata ttttttttct atacctatta
    25561 gatttaattc atactaaaaa cttacttttt aaattttatc aatatttaaa aatattttta
    25621 agtctagcca caaactaact atctaaattg aacaaaataa aaagaagtct attgacttct
    25681 ttataagctt acatattatc ttcttcttct taggaatgta ggtatctcca tgctctcatc
    25741 atcatcttct tcaattatac ttgccttttt aggaggctct attttttcct cagcaattac
    25801 ttcttcttca atttctttta cagcttgttt tactgttgta tttaatgaag aacgtatgct
    25861 tgatttaggt ttagtatcta aatctaaggc accattttgt aatccttcaa atcctgttgc
    25921 gattactgta atcattattt catcacctaa atcttctttt atagaagctc caaatataac
    25981 atttgcttct gggtcacagg attccataac taaagttgat gcctcattaa cttcaaataa
    26041 tcctaagtta ggaccacctg taacattaag aagtactcct ttagctcctt gtattgatgt
    26101 ttctagaagt ggagattgaa tagcttgtct tgcagcatca attgctctag tttcaccact
    26161 agcactacct atacccatat gagcaagtcc cttgtctttc attatagtag taacatctgc
    26221 aaagtccaag tttattaacc cttcaactgc tataaggtct gaaattgatt gtataccttg
    26281 ttttaataca tcatctgcaa ctgcaaaagc atctaacatt gatgtatttt tttgtactat
    26341 ctgtaaaagt ctatcatttg gtatcgttat aagagtatct acttttgatt taagttctgc
    26401 tattccacct tcagcgttct tcattcttat cttaccttca aatgcaaaag gtttggtaac
    26461 tacacctact gtaagtatcc ccatttcttt tgccaagcct gcaaccactg gagcagctcc
    26521 tgtaccagta ccaccgccca ttccggctgt tacaaagacc atatcagctc cttgaagtaa
    26581 ctttactatt tcatctttac tctcctcagc agctcttttc cctacttctg gatttgctcc
    26641 tgctccaagt cctctagtta atttttctcc tatttgaact ttatattcag cttttgatgt
    26701 atataaagct tgtttatctg tatttactga tataaattca acgcctttta gttgagcttc
    26761 taccattcta ttgacagcat tgtttccacc gccacctaca cctattactt taatttgtgc
    26821 acattcttct aattctacgt caaagtttag catcataaac ccccttctct catgattcaa
    26881 agttatcgta tgtattaatt atatcatttt cttcttttat tttgttaacc tttaagtaac
    26941 atattccaca aaaacgtaaa ttttcctttt taaatttctt acttatattg tcaaatatta
    27001 ctctttaagt ataaaataat ttacgtatat attaaatgct ctttaatttt tacgtttttt
    27061 gttcataata ttactaataa tatatcgtct aattatagat aagttattaa acaatctcat
    27121 accaaaaact agtacagcaa catagtaaag agaaactcct aacttttctc ctatataagt
    27181 caataatata gccaatatag cattacctat gaaaccagta ataaaaatta cattatcata
    27241 gttatcttca aaattggatt ttatcgctcc aaaaatagaa tccattattg caagaatagc
    27301 tactgacata tatatggaat aatccataga atatgtaaat ggaatatagt aacctactac
    27361 tacaccaaat gctaatcctc ctaaaaccca caacataaat cttcacctct ttattttact
    27421 ttgaaacaca actacattac aatccttttt attaattttt tttacttttt ctttattttt
    27481 ttgtattttt cgttttattt tgcaaataaa ttcactttat tcccattatt ttcctgaact
    27541 tttatattaa ttccatatat atccttaaga gatttagcat atgattctgg agagtttata
    27601 gctgcactca ataaatctat atctcctata gcttttatta taaatggttg tccatatgta
    27661 gtatcattta ctgtaatagt agctcctgaa cattttatct tacttgttgc tagtactctt
    27721 tccccattta tagaaattgc tcttgctcca gcctttttta aatcatttaa aactctcaaa
    27781 atatctatat catgtataat caaatcattt ggattctgtc cgtcttttaa ctctctctca
    27841 ctatccctca tagtaatagt tatgcctggc cctgtaacag ttgaataacc acttaattct
    27901 ttaacttctt caagctcttt ttgcataagt gtttggatag attcatttcc attctttttt
    27961 gcttctttat attcattcaa agtttttttg ttgtttttaa gagtttcttt taaatttttt
    28021 atttcttttt ttgtattttc tacctcactt tcaatacttt cagtttgacc taatgttatg
    28081 tatactctat ttgggtctag tgattttata taagttgata tgaaaatgcc taatatgaat
    28141 gctcctaata taactatttt ctttttcata ctattatttc ccctctgctt tgtttatatc
    28201 actatgacca tttatttcta tattgtcttc tttggtcact tttactttgg cattataata
    28261 tgtttctatt tcccagacaa taccatactt tatattaagt gccgaagcca atgtatcaga
    28321 atcaccaatt gcttttatta taattggttc ttttatgaca gtgtcattca tacaaagctt
    28381 gtcttctttt aaatacatat atgtattaaa aactattctc tggtcgttta tagatatggc
    28441 acttgcttgt gctgaattta atttatttat cattgacaat aataaatcat aattatatag
    28501 tatactatca tttttatcag agtttcctaa gtcttttgta gaatttatct caatttttat
    28561 gcctgttcct tcaacatctg tatatccagc taatgtttca tatttttcta tttctgactt
    28621 aagtatgtct tgttctcctt tattgttttt gcctctataa gtctctatat ctttcttcac
    28681 actattaact tctgattgta gattctcttt ttctgttttt aaagatttaa gttcttctgt
    28741 aagttgctct cctcttttaa aagttgtcat ccctctattt tctatattaa taattttaag
    28801 ttgtaaacta acaagaacac ccaatacaac acttcctatg attatacttt tatttataaa
    28861 tctttttttc aatatatcac ttccttacaa ttgtctatcc ttctggacta tacactgcca
    28921 actttccata gttaagattt atagtccctc cattagtatt tttattttgc aggtcaatca
    28981 gaattttact taccctagat atattataat ctaagttatc atcattagaa agtaagattt
    29041 ttatattact tcttgtaagc atttctatat tgctttcttt ttttaagttt acataattta
    29101 tttttctata aattccttct tcttttaaat aatttaaagt tttaaataca ttctccttag
    29161 ttttataatt tttaaatttt atagatttat tatcatcaac gctataatct acatctacta
    29221 tcaagtcttt tttactttca ttagaacctc ttagttcttc caataaattt ccttttttat
    29281 ctatataaca ataattatct tcatctttta atactgcaaa tatttctttt tcttttaaac
    29341 ttataattat cttattggga agtttt
//
LOCUS       DAELXX010000042        29336 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.042, whole genome shotgun sequence.
ACCESSION   DAELXX010000042 DAELXX010000000
VERSION     DAELXX010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29336
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.042"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            63..884
                     /locus_tag="KPH29_001782"
     CDS             63..884
                     /locus_tag="KPH29_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBG1467382.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
     gene            905..2101
                     /locus_tag="KPH29_001783"
     CDS             905..2101
                     /locus_tag="KPH29_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBG1467383.1"
                     /translation="MFIEFFYLLRSRGLNISLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSINYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMLSR"
     gene            2267..5665
                     /locus_tag="KPH29_001784"
     CDS             2267..5665
                     /locus_tag="KPH29_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBG1467384.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNTLLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVVTVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIISFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSSINQDLETEDIGYRSINKSFKDEENRYKFIDGYNIQI
                     HNDLENEIYISKNASRQSGILSGIDVVHTPEFEEELAGLGMSQNFFKISDSLGVLSIN
                     NTDYSSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICSGLGNVNSEYAGIAEDSELIIIKLGKIDGFYNS
                     AMLFAASQYAYKKAFELRRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPHVGGSVEVELELNEDEEELSLELWLNRPDKADVIIVSPTGEESKS
                     VGISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITVGAYNTINGSLWQSSSRG
                     PTIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            5675..7348
                     /locus_tag="KPH29_001785"
     CDS             5675..7348
                     /locus_tag="KPH29_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBG1467385.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPVDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSQLNIAINRND
                     GSLSQDNIGTGTLVSGILAGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     ISGEYDIYLPNKNLIAPDTRFLDPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            7622..7816
                     /locus_tag="KPH29_001786"
     CDS             7622..7816
                     /locus_tag="KPH29_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HBG1467386.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     misc_binding    7985..8218
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8295..9692
                     /gene="asnS"
                     /locus_tag="KPH29_001787"
     CDS             8295..9692
                     /gene="asnS"
                     /locus_tag="KPH29_001787"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBG1467387.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     gene            complement(9865..11040)
                     /locus_tag="KPH29_001788"
     CDS             complement(9865..11040)
                     /locus_tag="KPH29_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG1467388.1"
                     /translation="MNLEKIKKFFGFPLDKKHKENVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFATPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCDETDAKLKSECLRKII
                     ESCNFERDDVPLKCVTVSIGVYTKKIEKAITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            11459..12823
                     /locus_tag="KPH29_001789"
     CDS             11459..12823
                     /locus_tag="KPH29_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG1467389.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEIASLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLINPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIRPIIVFSIYY
                     AFLMGILALFSTFILDSNINFFNTTNYGTTLIMQFKVLQAIVISFVYSFVISLIGYKL
                     NSAD"
     gene            12845..13336
                     /locus_tag="KPH29_001790"
     CDS             12845..13336
                     /locus_tag="KPH29_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG1467390.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EKKAKIFLEMGILTYQKIREDSINDNSFDKFCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIICGYCESEIDTEAEFCPCCGRKIIM
                     DLE"
     gene            13609..14280
                     /locus_tag="KPH29_001791"
     CDS             13609..14280
                     /locus_tag="KPH29_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBG1467391.1"
                     /translation="MLDKVKGRLIVSCQALENEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMERLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            14365..15231
                     /locus_tag="KPH29_001792"
     CDS             14365..15231
                     /locus_tag="KPH29_001792"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBG1467392.1"
                     /translation="MKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGLYVGGS
                     TGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGYDAISA
                     VTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENEKIIGV
                     KFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRARQIFE
                     LTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKEATDEM
                     KSRAKEIYRKYF"
     gene            15264..16766
                     /locus_tag="KPH29_001793"
     CDS             15264..16766
                     /locus_tag="KPH29_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HBG1467393.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSVVLAGLTGIPVNVLIVV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFIGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            16896..17348
                     /locus_tag="KPH29_001794"
     CDS             16896..17348
                     /locus_tag="KPH29_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBG1467394.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEKGDFL
                     PLDGKNNGYMELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            17508..18311
                     /locus_tag="KPH29_001795"
     CDS             17508..18311
                     /locus_tag="KPH29_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467395.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NTGCTTLSKHYNIENKYVIWIQGESDAKYGSDALYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACNECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            18369..19256
                     /locus_tag="KPH29_001796"
     CDS             18369..19256
                     /locus_tag="KPH29_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBG1467396.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEENEISGICISTAGMVDPVEGKILFALEELIPNYKGMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEVWLGAGRGATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNINGSSFQELASTSSLIKKVAKIKNLNEKDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            19275..20123
                     /locus_tag="KPH29_001797"
     CDS             19275..20123
                     /locus_tag="KPH29_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG1467397.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            20186..20875
                     /locus_tag="KPH29_001798"
     CDS             20186..20875
                     /locus_tag="KPH29_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG1467398.1"
                     /translation="MSKLKDYNIDKVIIFNNLSTKTKNQLKEKAKLKRLQKKEILFYE
                     KDSLDSVYILLEGKVSIFKISENGERKVIFILNSGEVINDITFDSSKNSTVGCEAFEN
                     SIVAYYSIEEFLEIMQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVK
                     ETELSRYFKNN"
     gene            21265..22272
                     /gene="asrA"
                     /locus_tag="KPH29_001799"
     CDS             21265..22272
                     /gene="asrA"
                     /locus_tag="KPH29_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HBG1467399.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKEVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEIIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
     gene            22273..23064
                     /gene="asrB"
                     /locus_tag="KPH29_001800"
     CDS             22273..23064
                     /gene="asrB"
                     /locus_tag="KPH29_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HBG1467400.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            23077..24048
                     /gene="asrC"
                     /locus_tag="KPH29_001801"
     CDS             23077..24048
                     /gene="asrC"
                     /locus_tag="KPH29_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HBG1467401.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAVVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            24105..24881
                     /locus_tag="KPH29_001802"
     CDS             24105..24881
                     /locus_tag="KPH29_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBG1467402.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGLIFVGTGLVDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     STVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            25152..27170
                     /locus_tag="KPH29_001803"
     CDS             25152..27170
                     /locus_tag="KPH29_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBG1467403.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERISLIMERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEINYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSVLENLYEFLVDYKKV
                     KSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLR
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINAI
                     YKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDTNYISNYNVDKYDEG
                     RSVILESIVNTKSLGDKIICLNLLQTLNGVKEMYRISNITKVSIKEAA"
     gene            27385..28242
                     /locus_tag="KPH29_001804"
     CDS             27385..28242
                     /locus_tag="KPH29_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG1467404.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            28339..29208
                     /locus_tag="KPH29_001805"
     CDS             28339..29208
                     /locus_tag="KPH29_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG1467405.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
BASE COUNT        11633 a         2977 c         5261 g         9465 t
ORIGIN      
        1 atcaaaaata gtaataaagt ctatatacat ttagaaaata ctgtttaaaa ggagtaggat
       61 ttatgataaa aaaagagata tctaacttta gaggaagtag tgattatgta gtatcaccag
      121 aattgatggc atcagttaat gttgctattg ctcttgaaaa gccacttttg attaagggcg
      181 aacctggaac aggtaaaact atgctagcac aagcaatttc aaatgaatta aaaaaagatt
      241 tagtaatttg gaatataaaa tcaactacaa aagcacaaga agggctttat gtatatgata
      301 ctgttcaaag actttatgat agccaatttg gaggagaagg tgtagatgat attagcaagt
      361 acataaaata tggtaaacta ggagaggcct ttagctcaaa tcaacaagta attcttttaa
      421 tagatgaaat agataaggcg gatttagaat ttccaaatga cttgctttgg gaattggata
      481 aaatggagtt ttacataaat gagacaaaag aaactgtaag agctaaacaa agaccaatag
      541 ttattataac ttcaaatgcg gaaaaagaac ttccagatgc ttttttaaga agatgtatat
      601 ttcattatat agaatttcct gatagagaca tgatggaaga aatagttaaa gtacattttg
      661 ataaagtaga agaacaccta ttggaacaag taatgactac tttttattgg attagaagtt
      721 taaaggacat acaaaaaaag cctagtacat cagagttaat agactggata caagcattaa
      781 ctttgagtgg aatgcctatt gaaaaaatag aaaaagaagt tccttttgca ggaatactac
      841 ttaagaataa tgaggatatt gaatctatgc aaagacactt atagttaata ggagctggat
      901 aattatgttt attgaatttt tttatttatt gaggtcaaga ggattaaata tatctttaaa
      961 tgaatggatg actttaattg aagcattgga taaagggctg tgctattcaa acttttcaaa
     1021 tttttactat ctttgcagga tgattttaat aaaaagtgaa agtgattttg ataagtttga
     1081 tgcagtgttt ttggagtatt ttaaaggtat agaacaccaa gaagaaattc cagaagagat
     1141 tatgaattgg cttaaaaaac cagatataga tttagaagag tttgaaagat tggaacaaaa
     1201 tccaaatata aatcttgata aattaagagc taaattggag gaacgaataa aagaacaaga
     1261 ctctcctcat aatggtggta attattggat aggcacagga ggcacttccg agttaggaca
     1321 ttcaggaaaa gggcaaactg gtatacgtat aggtggaaat tctacttatg gtagagctgt
     1381 tcttgaagtt gcaggagaaa gaaaatacag agattttaga gatgatgaag ttttaaatat
     1441 gcgtcaattt caagttgcac taaagagact tcgtcaattt tctactagaa tagatgctcc
     1501 aaagacagag cttgatttgg ataagactat tgaagagact tgtaataatg caggatattt
     1561 aaagttattt tttgagaagc caagaaaaaa tacagtgaag cttttattgc ttatggattc
     1621 aggtggttca atgagggggt atagcacttt atgtaataca ttatttcagt ctgtaagtaa
     1681 atcaaatcat tttaaagatg taaagatata ttatttccat aactgtattt atgatagatt
     1741 atttacaaca cctgaatgtt ggttaagtaa atctattaat actgaatgga tattaaaaaa
     1801 tatagataaa aactataaag ttataattgt tggagatgct tctatgtcgc cttctgagtt
     1861 acttcatatt ggtggtaact atcgtggtcc atataattac acacctggta tagaatggct
     1921 taaaaggttt aagagaaagt acagtaaaat tgtatggatg aatccagaac ttagagatgg
     1981 atgggattca attaattatt ggtaccaaac tcaaagaatg atacaaagtg aatttaatat
     2041 gtttccactg actgtaaaag gtcttgaaaa agcattgaaa aatttaatgc tttcaagata
     2101 aagtaaatat aaatgatggt atgatttatt caaaattgta ccatcattat ttatttttta
     2161 atatatctat tataaattgt ataactgaaa cttaaaaaat tatatagtct aatacatttt
     2221 agacttacac aaaacatata cattaataat aattttagaa gtcgaggtga ttataataaa
     2281 ttatgaatta attgtaaaat acaatggaga tatattgaga ttagaagaag aattaggtgt
     2341 atcagtagag atacttaatt catcatatgc aataataaca tcaagtaatg aagaagatgt
     2401 aaatacactt ttaacttatc cagaaattga atttatagaa aagcctttta tattacagac
     2461 tcaggatgtg caaagttttt caagtacagg aataacgggg tttaaaaata gaactggttt
     2521 aactggaaag ggaactataa ttggtataat tgattcggga atagattata ctctacctgt
     2581 gtttagagat agtgatggga ggtctaagat attatattat tgggaccaat ctattcaagg
     2641 aaatccacca gaaggtttta gagaaggaac attatatact aatgaagata taaataatgc
     2701 aatagatgga agtatgtata tacctatttc aacaactagt ttacatggga cacatgttgc
     2761 aggtatttgt gcaactattg caagtgatgc cagaataata gtggttagag ttggaaatat
     2821 acagaccgat attttttcga gaagtacaga gtttatgagg gcaattaaat ttattttaga
     2881 tagggcatta gaattaagaa tgcctgttac attaaatata agttatggaa gtaatgaagg
     2941 ttcacataga ggaacatctt tatttgaaca atatatagat gatatgtgtt tattttggaa
     3001 aaacaatata gttgtagcag ctggaaataa tgcagataaa ggaggtcata aaaggattag
     3061 gcttcaaaat aatattacag aagaagttga gtttatagtt ggggaaggag agcgtatatt
     3121 aaacataaat atatggccag attttgttga tgattttagt gtacatttgg taaacccatc
     3181 aaacaatcaa acacaagcaa tctctctaac ttcaggtgag ataagaaata ctttgggtga
     3241 aactagaata actggttatt tttaccctat agcaccgtac tctcttacaa gaagagttac
     3301 tctacaatta agttcaaata cacagataac tcctgggtta tggaaaattg tatttgaacc
     3361 aatagatata gtaactggta atgtaaatat atatttgcca acttctgaag gactaaatag
     3421 aaatactaga tttctgattc ctactcaaga gcttactgta acagttcctg gaacagcaag
     3481 tagagttgta acagtaggta gttttaattc aagaacagac atagtatcta tattttctgg
     3541 tgaaggagat acacaattag gtgtttttaa acctgattta ttagctccag gtgaagatat
     3601 aatatctttt ttaccaggag gtacaagtgg agcattaact ggaactagca tggctacacc
     3661 tcatgttaca ggggtttgtt cattgtttat ggaatgggga attgtaaatg gaaatgattt
     3721 atttttgtat tcacagaaat taagggcatt acttttaaag ggagcaagga gattaagtaa
     3781 tcaatcttat ccaaataatt catcaggatt tggtttttta aatttatcag atatagattt
     3841 gtacactcta tctagtataa atcaagattt agaaacggag gatattggat atagaagtat
     3901 taataagtca tttaaagatg aggaaaatag gtataagttt atagatggtt ataatataca
     3961 aatacataat gacttagaga atgaaattta tataagtaaa aatgcaagta gacaatcagg
     4021 aatactatca ggaatagatg tagtccatac tcccgaattt gaagaagaac tagctgggtt
     4081 aggtatgagt caaaattttt ttaagataag tgattcatta ggtgtactat ctataaataa
     4141 tacagactat agtagtatac aacgagtttt gcaacttcct tcaatcataa gaactgtatc
     4201 gactacaaag atgacattac ttggagaaat taatcgaggt acttttggag gtgtagttgc
     4261 aacagaagaa atgggcgtaa atttctttaa aaataatcct aatattaata taacaggaag
     4321 agggactcta atatcaattg cagatacagg aatagactat ctacatccag attttattta
     4381 tccagatggt acttcaaaaa tagtatattt atgggaccaa acaaaagaag gaactccacc
     4441 tgatggtttt tatattggga cagaatatac acgagaagat ataaatcgag ctatcgcaga
     4501 aaatgaccct agtctatctc aggatgaagt tggtcaagga actatgttga gtggtatatg
     4561 ctcagggctt ggaaatgtaa atagtgaata tgcaggtata gcagaagatt ctgagctaat
     4621 aataataaaa ctcggtaaga tagatggttt ttataatagt gcaatgttgt ttgctgcatc
     4681 tcagtatgca tataaaaaag cttttgaact tagaagacca ctagttataa atatgtcttt
     4741 aggtaccagt agcttggcag gtttaaccaa taggagcaat agtgaaaaag cattctttac
     4801 tagagggtta tgtataacag caggagcagg aaatgagggc aatacacaga cacatacatc
     4861 tggaataata ccacatgttg gaggttctgt tgaagttgaa ttagagttaa atgaagacga
     4921 ggaagaatta tcattagaac tttggttaaa tagaccagat aaagctgatg taataatagt
     4981 atctcccact ggtgaagaaa gtaaaagtgt tggaatttca aattataata aagtaacagg
     5041 tttatttgat ttagagggga ctgaatattc tataacgtat atatacccaa caactttttc
     5101 tgggcagcaa tttactaatg ttactttaaa gaatgcaaaa agaggtgtat ggaagattcg
     5161 attagtaggg gtttatatta taacaggaag atataactta taccttccaa atagagaact
     5221 tttaaaaagt ggaactagat ttagagaggt agaccctttt tacactataa attatcctgc
     5281 tattcaagat gatttaataa ctgtaggagc ctacaatact attaatggta gtttatggca
     5341 aagttcatca agaggaccaa ctattgaaga caggttaaaa ccagatatag tagcaccagg
     5401 tgtcaatata atagctgctt atcctgggaa cacgtatgca actataactg gtacagcagc
     5461 agcaagtgca catgcagctg gtgctgctgc aatgtatttc caatatactt ttgttgatgg
     5521 tagatatcca aaccaagcat atgtgcaaaa aataaaaact tttatgcaag ctggtgctag
     5581 aaaagattca aacacagtat atccaaatac aaattctgga tatgggcttt tggatgttag
     5641 aggaatgttt gatgttttaa gataggaggg atttatggaa aaatcttatt gtataattta
     5701 tcaaggtgat atagaatctg ccctacaaga aaatggaata aataggtata tggttttaaa
     5761 tagtcagctt gcagtaatat atgtacctgt agattttgat gaaactattt taaataatat
     5821 aatacaagtt gcttggtggg aagaatctga accaatgagc agtctaattg aaataactaa
     5881 taatgtaaat aatggagaaa ccattacaac agcagctgaa actgattata tttatgaaaa
     5941 tccatataat gatataacag gaagaggaat tttattagca gttatagatt ctggtataga
     6001 ttatttgcat cctgatttta taaatgatga tggaactagt aaagttctat atttatggga
     6061 ccaagaagca aatacaaatc cacctccaga aggttttatt tttggaagtg aatttacaag
     6121 aagtcagtta aatatagcca taaatagaaa tgatggaagt ttgagtcaag ataatatagg
     6181 tactggaact ttagtttctg gtatattagc tggaaatgga aggataaatt cgcagtatag
     6241 aggtattact acagaatcag acttaatagt agttaaatta aaatcatata cagatactta
     6301 ttatgctgga aggataaatt atagtgtttc agatttttta gcagctataa cgtatgttac
     6361 taatatagca agaacagaaa ataaaccact aataataaat ttgactattg gagttaagtc
     6421 aagtgctgta gcaactactt caatattaga tacttttaat atattgagta gtgctggtgt
     6481 ggtagtagtg agtggtgcag gaaatcaagg gaatacagat attcattatt ctggcagatt
     6541 tagcagtgtg ggagaagttc aagatgtaat aatacaagat ggagatgact atgctcttga
     6601 tataacacta aatacgaatg gtccagataa agtgggagca cagataatat ctccttcagg
     6661 tgaagttagt catgatataa ggtattctcc agacttttat atatatagag gaaaatttaa
     6721 cttagaaaat actacttatg caatgagatt tatttatcca tatattacct ctggaaaaga
     6781 aaatttggag ataagattaa gagatataaa acctggagta tggattttaa gattgacttc
     6841 agaacttata ataagtggag aatatgacat atacttacca aataaaaatt tgatagctcc
     6901 agacacaagg tttttagacc ctgattcagt agctactata actatgtatg ctgctagtga
     6961 tgatgtaatt acagttggta catttaataa taagactgat agtatgtgga ttggttcttc
     7021 gaaaggacca attagaggaa gaggaattaa gccagatata gtagcttcag gtgtagatat
     7081 aatatccaca tataaaaatg gaacatataa tacaggaaca ggaacaggag tgagcagttc
     7141 tatagtcaca ggtgttttag ccttattgat ggaatactta gaaaaacagg ataatgtacc
     7201 gagattatca ttgtttacac aagttttaaa aacatattta atattgggtg ctacaaaact
     7261 tgagatatat acatatccaa atgtatcgca gggatatggg attttaaatt taaaaaatac
     7321 gattcaacag atagcaaata ctctatagat aagaacctat gtaaaattat aattgattag
     7381 taatttaatt gtatcaaata ctttagatag agtagaaaaa tggcataaaa aataaatatg
     7441 aaaattaaaa aaacagtttt atattttgaa tacacaaagt aaatattttg tatataaata
     7501 ggtataaatt aagtattgaa aagatatgat gttaatttaa aaatcattat aatttagact
     7561 tatgaatttt atttgtaaaa ttgctaaaac tattacaaaa taaataatag aggtgatagt
     7621 tttggaaagt aaaaaggcaa gagcattaaa aggtaaagat aataaaagac ttaaaagttc
     7681 acttttagtt ggaaatgagt cgacagcagc atgggctgat gttgaaaaat taaaacctca
     7741 tagtaaagtt tcaatacctt caattaaggg tgtcgaagaa gcaaaagact gggttgacaa
     7801 tggaagtaaa ttataaaatt ttggaaattt aagtaataaa gaagtaatga agataagtat
     7861 ttattatcat tatgcaaggt agaattatta tattttaaat aacaatcagt atcaattata
     7921 taaatttatt gactaaaaat ttataaattg gtataatttt gttaattagt agaaataata
     7981 aaagataaat actgtgaaga gaagagtaga tataaaatgt agttctagcg agttgaggat
     8041 ggtgaaagct caatacgagg tttatattga agaacatctt ggagtttcta atcgaaagct
     8101 tatttagtaa gtagacttag acggaaggca ccgttatagg ctttaagcat tgcttaacta
     8161 agaggtcatt atgacaacta gggtggtacc gcgagagtaa tagtctttcg tccctttata
     8221 tagggatgaa gggctttttt tattgaaatt atatttaaaa gaaattaata acaattatac
     8281 taggaggatt taaaatgcaa aaggaattta taacagtaaa agaattgtac agagaaaaag
     8341 aacagtacat agggaagact gtaaaagtag ctggatggat aagaacatct agagtatcta
     8401 aaaattttgg atttatagag ctaaatgatg gaagcttctt taaaaatatg cagatagtat
     8461 ttgatgagaa agtagaaaat tttaaagagg taggaaaatt agcaataagc tcatctatac
     8521 ttgtagaggg agagttagta tctacagaag gggctaaaca accagtcgaa atacatgcaa
     8581 aaagcatagt tgtagaaggg gaatcagata gttcgtatcc actacaaaag aagagacata
     8641 cgcttgaata tttaagaacg atagcacatt taagaccaag aagtaatact ttttcagctg
     8701 tatttagagt aagatcaata gctgcctatg caatacacaa attcttccaa gaaagaaact
     8761 ttgtatatgc acattctcca atcataacgg gtagtgactg tgaaggtgca ggagaaatgt
     8821 ttagaataac aactatggat ttaaatgata ttccaaaaac tgaagatggt aaaatagact
     8881 atactcaaga tttctttgga aaagaggcta atttaacagt tagtggtcaa ctaaatgccg
     8941 agataatggc actttctttt agaaacgttt atacttttgg acctacattt agagctgaaa
     9001 actcttatac aggaagacat gcttcagaat tttggatgat agagccagaa atagtttttg
     9061 cagaccttga agataacatg gaacttgcag aagatatgat aaaatatgtt ataagctatg
     9121 tattagaaaa tgctcctgaa gaaatggaat tttttaatag ttttatagat aaaggattgt
     9181 tagaaagatt aaataagata gtaaactctg agtttattag aataacgtat actaaagcag
     9241 tggaattatt aattgaatct ggtcatgaat ttgagtatcc tgttgagtgg ggatgtgacc
     9301 ttcaaactga gcatgaaaga tatataacgg agcaaatata taattgtcca gtgtttgtta
     9361 cagattatcc aaaagatata aaagctttct atatgagaat gaatgaagac ggtaaaactg
     9421 ttagagcaat ggatttatta gttcctggag ttggagaaat cgtaggaggt tctcaaagag
     9481 aagaaagaga agatgtattg ttagatagaa ttaatgaaat gggattaaga gaagaagatt
     9541 attggtggta tttagaactt agaaagtttg gtactgctac tcactctgga tttggtcttg
     9601 gatttgaaag aataattatg tatatgacag gtatgtctaa cataagagac gttatacctt
     9661 tcccaagaac tcctaaaaat gctgaatttt aataattata tataataaaa cttatataac
     9721 tttaagtata aacactaaaa tgacttgcat atttatctat tttagagata tgcaagttat
     9781 ttttattgta ctataaatat ataatttgat tacttttatt ttgcgatata atacttatag
     9841 gtttatataa tagtatgtaa ctttttaata aaatgcaaca caattacgtc cctgttcttt
     9901 tgcagtatat agagccttat cacaattact tataaactca tttaatgtta ttgccttttc
     9961 aattttcttt gtataaactc caatactaac agttacacat tttaaaggaa catcatcacg
    10021 ctcaaaattg caggactcta taatttttcg taaacattct gatttaagtt tagcatctgt
    10081 ctcatcacag ttaaaaatac atataaaaaa ttcctcacca ccatatcgta tagcatgttc
    10141 attttcagaa cttgtaaaat ctaaaattac tttggcaata ttttgtagac aatagtctcc
    10201 cttttgatgt ccataattat cattaaattg tttgaaataa tctatatcta tcatcattcc
    10261 agttataggt tcttgatttg catggctgat taattttttt tgtagaatat cttcaaggta
    10321 tctacggttg ttcattttag taaggtcgtc tgtcataact ttgtcgttaa gtaaattatt
    10381 tatatgagat atttcttcat attgttgact aattaaaagt ttatcatagt aagtaaatac
    10441 acgatttcta tatagcattg tagaaacaaa aatggcaagg gatgttataa aaaatgtgtt
    10501 gacaatatta ctaaactcat tgcctggtat agtttgaatc ccaggtaaga ataaattaag
    10561 aaacaaaaag catcctccaa aaactattac tgattctcca aattttaata ctgagaaagc
    10621 tgcagctgct aacataatat aactaaatac acttaggtca tttccaccta actggtcaag
    10681 tagagtaatt acacaacccc ataggcagaa aaaagtcaca taaattatcc ataaatttat
    10741 ataaaacgta caacaatcct tttttttatt ccaaagataa gaaaatacaa acataaagac
    10801 agaagataca caaaatagtg ttacatacat ataaaaatac cattggcgtc ttggtgtggc
    10861 aaaagggcct ccttctctag ctgagattga tatcatcata attatttgag atattactat
    10921 tattatgcaa atgattttac ccgtatgata aagataattg cagagttctt tttcaacatt
    10981 ttctttgtgt tttttgtcta gaggaaatcc aaaaaatttt tttatttttt ctaagttcat
    11041 ataagcctcc attaaaaata aagcataaaa ataagtattc ctttctatca ttagatattt
    11101 ggaatacttc gtttatgatt taattatatc aaaatatatt gtgtgtaaca atctaaaatt
    11161 gtgatagata aaatttatta gctaatgttg agtaacttta ttaattaata ctttactata
    11221 attaagattt acagatagtt tgctgctgaa aataaagaat agaataaatt taaattaagc
    11281 ataataaaat ataaataata aagttcgcat taacaaacta tttaaatatc aagacctgtt
    11341 aaaaaacaac agcaataaac aaataaaata gataaatata tccaattaag gtataattaa
    11401 gttttatatg atatagttat atttgataat ttacaatagt aaaacttgga gggaaaaaat
    11461 gggtgaaaag aaaataaata atatgaatta ttcacataat aattattcca aaaagacagg
    11521 tgaaccaact agaaaaataa aaaatagcca tatcactctt aaaagtaaag aagaaaaaat
    11581 ttgttctagt tgcaataaag aaaataatac taaggataat tactgtaaat tttgtggaaa
    11641 tgaactttat gaaatagcat cattaagacc acttgaaaca aaactagact tgaaatctaa
    11701 aataaaagag ctttcatatc atgcaaataa aagaggtgta tttttaacaa catttacaac
    11761 catatttata ttgtttatta ttgcattgat atttaaagct attataacta ttcaatttaa
    11821 tgatattagt tatttaataa atccagcaca tataatccta gctctcaact tgggtcaaat
    11881 tagtgtatct atgtctacca tgatgggttc tgggtttata aatgccaata taggtttgtt
    11941 gatattactt ataatcccta ttttggtatt attaatatca aacctaattt ttatgagaaa
    12001 aagatgtaga gattcaaaaa ctgtattagc aaattctcta ggagttggaa ttttttatgg
    12061 attagtttta gctattttat ctatttttac taatgtaaaa actagttcac atagtatgtt
    12121 agaatatgga tatgcattag aatatagtta tgaatttttt agtgttcttt taaatggatt
    12181 tgtattaggg tttatatgta catacataac tacttataaa aaaagttatg aaaaggaaaa
    12241 tatgtattta tctctttttt caaattctat aagaacattc atattaggtt atgttttagt
    12301 attagtaata ctattggtac taactatatc tgatagtagt tacttaaatg aactagatat
    12361 gagtagttat tcaaatggat taaatttatt tactatactc ccacaaatag ctagttatat
    12421 gtgggctttt gcaaatggaa tttcagtaac cataataaac tcaacagtat caatgtttac
    12481 tttaagttct tcatctttat ttggagatac taaattaatg ttttatgcaa tgggagcatt
    12541 gtctatgtta atattattat taaatggata caagctaaga tttaagtata atacagatag
    12601 tataagacct ataatagtat ttagtatata ttatgcattt ttgatgggaa tattggcttt
    12661 atttagtaca tttatactag atagtaatat taatttcttt aatacaacta attatggaac
    12721 aactttaata atgcaattta aagtcttaca agctattgta atttcatttg tatatagttt
    12781 tgtgatatct ttaataggat ataagctaaa ttcagctgat taaatatgat tggagggaat
    12841 ttctgtggaa ggaaatacta ataataaagt agatgataaa ttttctaagg ttaagaaaaa
    12901 gtttggtagt ggaaaattta aagttgaatt atataaagaa aaatcaaaat taaatagaga
    12961 gattgatgat attcaagaaa agaaagcaaa gatattcttg gaaatgggaa tactgacata
    13021 tcaaaaaatt agagaagatt ctattaatga taatagtttt gataaattct gtgatgagtt
    13081 actagaactt gataagttaa tttatgaaaa aaatatggaa attaatgaaa tggaaatggc
    13141 agaaagtaat gtaacttgtg agtgtggtta tgttggaaat gtaaatgata aatattgtgc
    13201 tgaatgtggt aaaaaatttg aattggaaaa tgaatgtgat gattttataa tttgtggtta
    13261 ttgtgaaagt gagatagata cagaggctga attttgtcca tgctgtggaa gaaaaattat
    13321 aatggattta gaataatatt gggaaaccta tgtatagtat acatgggttt ttttattgaa
    13381 attatttaaa tggacttata acagtagtta tataaaatga aaataaacac caaaaaaatg
    13441 aaataaaatt tcttttaaat gtaaaaaaca agctaatata taaaataaat cagtaaaaat
    13501 atagatgcga gaaaaaaata tgaaaaaata ttgcaaagaa aaagttttca tgttattatt
    13561 aatacataaa tcaagaaaat aaatttcaga aacgaggtaa tttaaaaaat gctagataag
    13621 gtaaagggaa gattaatagt atcatgccaa gctttagaaa atgaacctct tcatagtcct
    13681 tttataatgg ggagaatggc taaggctgct atggaaggtg gagctgttgg aattagagct
    13741 caaggagttg aagatattat tgaaataaaa aaagttactg gacttcctgt tataggtatt
    13801 ataaagagaa actatgaaga ttcagatata tatataacgc caactaaaaa agaagttgat
    13861 gaacttttaa caacagggtg tgaaatgata gcattagatg ctaccaatag agttagacca
    13921 aataatgaag acttaaaaga acttataaag tatataaaag aaaacggtgt tctagtaatg
    13981 gcagacattt caaattatga tgaggctatc aaagcacaag agtacggtgt agattgcgtt
    14041 tcaactacac tatctggata tactccatat actaaaacat tagaaggtcc agattttgta
    14101 ttaatggaaa gattagttaa agatttagaa atacctgtaa tagctgaagg aaaggtaaat
    14161 actcctcaag atttaaaaaa ggtctttgag ttgggtgttc attcatcagt agtaggttca
    14221 gcaataacaa gaccacaatt aataacagaa aaatttgtta aagcaattga aattaattaa
    14281 aaattaattt ataaattaaa aaattattat atttaactat acttgaaaat taaaattgga
    14341 ggtaaagata tgagaacaac agatatgaag ggaatttatt cagcattatt agtttcattt
    14401 gataaggaag ggaatataaa tgaaaaaggg ttaagacaaa taattagaca caatatagat
    14461 gtttgtaaag tagatggtct ttatgtagga ggaagtacag gggaaaactt catgctttca
    14521 actgatgaaa agaaaagaat atttgaaata gctaaagatg aagtgaaaga agaaataaaa
    14581 ttaatagctc aagttggttc agttaattta aaagaagctg tagaacttgc taaatttaca
    14641 actgatttag gatatgatgc aataagtgct gttacacctt tttactataa atttgatttt
    14701 gaagaaataa aacactatta taatacaata ataaattctg tagataatag attaataata
    14761 tactctatac catttttaac tggtgttgat atgagtttag accagtttgg agaattattt
    14821 gaaaatgaaa aaataatagg tgttaagttt acagcagcag atttctactt attagagaga
    14881 atgagaaaaa cattcccaaa taaattaata tttgcaggat ttgatgaaat gatgttacca
    14941 gctacagttt taggtgtaga tggggcaatt ggttctacat tcaatgtaaa cggtgtcaga
    15001 gctagacaaa tattcgaatt aactaagaat gaaaaaatat cagaagcact tgaagtacaa
    15061 catgtgacta atgatttaat aacagatata ttaggaaatg gcttatatca aacaattaaa
    15121 ttactacttg aagaacaagg tgttgaggct ggatactgta gacaacctat gaaagaagca
    15181 actgatgaaa tgaaatcaag agcaaaagaa atatacagaa agtattttta ataaaatact
    15241 tttagtatgg gaggattagt caaatggtag gatttacttg gatagatttt gcaatactag
    15301 tggtttactt attggcagtt ttatttgcag gattgttatt ttcaaaaaag gaaatgaagg
    15361 gaaaagagtt cttcaaaggt gatggaacta ttccgtggtg ggttacatca gtatcaatat
    15421 ttgccacatt attaagtcca atatcatttt tatcattagc aggaaattct tatggtggta
    15481 cttggatttt gtggtttgca caattaggta tgtttatagc aatacctttg acaataaaat
    15541 ttttcttacc tctgtatagt aggttagaaa tagatacagc atatgaatac ttagaaatga
    15601 gatacgaaag taaaggtctt agagtacttg gagctttgat gtttataata tatcaaatag
    15661 gacgtatgtc tatcataatg tatcttccat cagtggttct agctggtctt acaggaatac
    15721 ctgtaaatgt attgatagta gttatgggtg taatagcaat aatttattca tatacaggtg
    15781 gattaaaggc tgtcttgtgg acagatttta tacaaggtat ggtattaata gtaggtgtta
    15841 cagggacatt gttctattta atagcaagta taaatggtgg ttttggaaca gtaatggata
    15901 ctcttacaac tggacacaag ttcttagcaa gtaatgaagt tatgtttgat aaaaatatac
    15961 tatctacaag tgcatttata atatttattg gagctggtct taatactttt tcatcatatg
    16021 tatcgagtca agatatagtt caaagattta ctacaacaac tgatataaag caacttaata
    16081 aaatgacata tggaaatggt gttttatcaa tgggacttgc aacagtattt tatctaattg
    16141 gaacttgttt atttatatac tatactcaaa acccagattt agctcaaaca gttcaacaag
    16201 accaagtatt tgcatcatat atagcttatg aattaccagt gggtataaca ggaatactac
    16261 ttgcagcgat atatgcagct tctcaatcaa ctttatcgac agggcttaat tcagttgcta
    16321 caagttggac tttagatata caaactatca taacaaaaga tataagtatg gaaaagcaaa
    16381 ctaagatagc acaatatata tcattaggtg taggtatact ttctatagct gttgcaatag
    16441 tattagcaaa tggagaaata aagtcagcat atgaatggtt taatagtttt atggggctag
    16501 tactaggtgt tttagcagga atatttgtac ttggtgcatt ttgtaaaaaa gcgactaaaa
    16561 tgggagcata tgttggattc atagtatcag caattttagt tgtttattta aaataccgta
    16621 tgccagaagt aacttcttgg gcatattctc taataacaat cacgacttct gtagtggtag
    16681 gtacaatagt tagtgcaata gaagctaaag ttaaagcaaa agtatcttta ccaaaagctg
    16741 agactactgt ttattatgaa aaataatatt taaatgataa attgtcttat agagtgatta
    16801 gataaataat ttagttactc tataagattg tataatatac tttgtatata aaagtataaa
    16861 aatagacttt ataattatat agggagatag tgcttatgat atttggaaat ataaatcata
    16921 gtaagacata ttcaacattg catgaagatt tacttagatg ttttgaatat gctaaagata
    16981 attcattaat agattatgaa aaaggtactt atacaataga tggtgatgat atttttgtga
    17041 atattgtaga gtataaaact tgtgaaaaag aagatagatt ctgggaagct cataagaagt
    17101 atatagatgt tcatttgatg cttgatggat gtgaacgaat agatataaat tttatagaaa
    17161 atttagaaca aaaaatatat gaagaaaagg gcgatttctt gcctcttgat ggtaaaaata
    17221 atggatacat ggaacttaga aaaggtgatt ttttagtttg ttatccagaa gatgctcata
    17281 tgacaggaat aaaagtttta gaaaaagaaa atatcaaaaa agctattttt aaggttttgg
    17341 ttagataatg ataatttata taaccactag ggctgaaaat agtgatttaa ctttcagcga
    17401 ttagtgagac ttcgtaaatt ttagttagat tactattttc agccttagtt ttttatgaga
    17461 aaattgaaaa taattaaaag cttaaaattt tgtaaaggag ttaacttatg gaaaatatag
    17521 atgtattctt attaatagga caaagcaatg ctagaggatt gggaaatcca aaagagagca
    17581 tcattccaaa tgaaaattgt tttgaatatt tgagtactga tgaaattata aatatgagat
    17641 gtgagctaga gacttcagaa ggtgatggta ctatagcacc tgcattttca aatgaatgga
    17701 ataagcttac tggaaataaa gtttgtttta ttcacaatgc aaaagatggt tcaagaataa
    17761 aaaactggaa tcatgataat aactggtttc taaatgacac tatagaaaaa tttaatacag
    17821 gctgtactac cttaagtaaa cattataata tagaaaataa atacgttatt tggattcaag
    17881 gtgaaagtga tgcaaaatat ggtagtgatg cattgtacta taaagaaagt ctaaaaaaaa
    17941 ttgcgtatag gcttaaagaa gaatgtatga ttgataaaat gtttgtaagt ttaactggtt
    18001 attggcttgg agaagatgaa tattttatta gaacaagacg aattgcagca gctcaagaat
    18061 ctgcttgtaa tgaatgtgat atactgtgtg taggaagtaa aattgctatg caatttcatg
    18121 acagaggatt aactattgat gatgttcatt acactcaaga aggtcttaat atgttaggag
    18181 aggacttatg taaaaatatt tataagtatc atataaccaa agaaaaaagt gtattaagtg
    18241 atactataga cttatcagaa gcaaggaaat atatctgtga attggaaaag ataagtaaaa
    18301 gatttgtgta atataaaatt ttaaatagag ttgttaattt ataaaaataa tttgtatgga
    18361 gatatgttat gaaaaattac gtttgtatag atgttggagg tacttcaata aaatatggtt
    18421 ttattagaga agatggattt ataattgata aatcaagtct tgatactgaa gcaaaggaaa
    18481 aaggtggaga aggaatatta gccaaaataa aagatatagt aaaaaaatat atagaggaaa
    18541 atgaaataag tggtatatgt atatcaactg caggtatggt tgacccagtt gaaggtaaaa
    18601 tactatttgc attagaagaa ttaattccaa attataaggg tatgcaatta aaaaaagaag
    18661 tagaaaaaga gtttaatata aaatgtgaag ttgaaaatga tgttaattgt gctggacttg
    18721 gtgaagtgtg gcttggcgca ggtagaggtg caacatcttc tatatgttta acaataggta
    18781 ctggaattgg tggatgtatt ataataaaca ataaactgat aaatgggttt agtaatagcg
    18841 ctggtgaagt aggatatatg aatataaatg gaagttcttt tcaagaattg gcatctacta
    18901 gcagtctaat taaaaaagta gcaaaaataa aaaatttgaa tgaaaaggac ttaaatggaa
    18961 aaataatttt tgatttagca aagaataatg atgaagattg tttaaaagaa ctcgataata
    19021 tgataaaatc attagcagtt ggaatcgcaa atatatgtta tgtaataaat ccagaagtgg
    19081 taattttagg tggtgggatt atggcacaag aaaagttcct aaaaccaaaa atagacaaag
    19141 cattaaagga ggttcttata gagagagttt acaaaaacac taatatagaa tttgcaaaga
    19201 gacaaaatga tgcaggaatg cttggagcat tatataattt tttaaacaaa atataaagaa
    19261 tagaagggtt tttaatgagt attttagaac aattggaatc accaactttt aaagtcacaa
    19321 aatcagataa attattaata gcttatataa aagaaaatat agatgatgta ttttataaac
    19381 caatatcaca aatagctaaa gagagcaata ttggtgaagc aactataact aggtttgtaa
    19441 aaaaaatgaa ttttaatgga ttacaggatt ttaaagttac tttagcacaa gaaatatcaa
    19501 ctatcaataa aaaaaatatt ataaataaaa atatacaaaa tgatgagcca gctttggata
    19561 cagcaaaaaa acttttaagt tcaaatgtaa ccactttaga gaacacagta gagataatca
    19621 atagtaagga tgtacatgac tgtgcaaggc ttataataaa tgcaaagaaa gtttacttca
    19681 taggcattgg atattctggt ataatagcac aggattcaaa ctataaattt atgagaatag
    19741 gccttaattg tgtaagtttt gatagcagtc atacgatgat aatgatgtct tccataatgg
    19801 aagaaggaga tttaataatt gcaatttcac attctggtga aacagaagag attataaaaa
    19861 ctgtaaagct tgcaagagcc aataatgcta aaattatatc aattactgaa aataaaaatt
    19921 ctgaattaaa ggatatttca gatgtacact tatcttatgt atcaggagaa acagtactgg
    19981 aaactggttc aatatcttct aaattagcac aatttttcat tattgattta gtatatacac
    20041 aggtagtaaa agaactttct aatgaagcta ttgaaaggaa aattaaaact acaaatgcta
    20101 taaaattgtt taaaaatgaa taaaataaga aatttgtaat gttttataga gctctcttaa
    20161 aaatttagga atggagagat attacatgtc taaattaaaa gattataata ttgataaagt
    20221 aataattttt aataatttaa gtactaaaac gaagaatcaa cttaaagaaa aagctaaact
    20281 aaaaagatta caaaaaaaag aaattttatt ttatgaaaaa gatagcttag acagtgttta
    20341 tatactttta gaaggaaagg tatctatttt taagataagt gaaaatggag agagaaaagt
    20401 aattttcata ttaaatagtg gggaagtcat aaatgatatt acatttgact cttctaaaaa
    20461 ctccactgtt ggctgtgaag catttgaaaa ttcaattgta gcttattata gtattgaaga
    20521 atttttagaa attatgcaaa atgattttca acttacaaag gatattattt cttatatgga
    20581 aagaagaata agacgtttat atagacagtt gaaaaattct atatccataa aagtagataa
    20641 aaaacttgca gcaaaattat ataggttaag tagagaattt ggagtttgct gtggagaatg
    20701 gactttacta aatgtaaata ttacagttac atatttagct gatatgcttg gctgtaaaag
    20761 ggagactgtt tccagaatga taaaggtatt gcaaaaagaa gaacttatta agattgataa
    20821 taaaggtttt tatgttaaag aaactgaatt atcaaggtat ttcaaaaata attaattatg
    20881 attaagtaaa agtattatta gaattttaat taaataaaaa tattattaag agaatctatt
    20941 tatagattct tttttattgt ctaatttatt aatttttatt ttaccctatt gaattgtata
    21001 aaaaagcaaa taagattctt tattattcaa aaaattgagt agataatagt tttattgtat
    21061 ttaatgattg tatctaagtg attagatttg aaatcgaata atatagataa taaaaaaata
    21121 ttaacagaga ttagtaaact ctattaatat ttttttatta tttttgaaac tgtgataatt
    21181 atcacagaag catagaaaat aaagtgttaa gatttaaaca agatagcaag taagtaaaac
    21241 taaaatataa gaaaggtgat aaaaatgaaa ctaaaattag attcatctaa gttcaatgaa
    21301 ggtctgaatt atctaaaaaa agactacaag atatttgcac cagttttaat cccatttaaa
    21361 ggtacttttt cagatactga tgttataaga tacaaggagg taaacgcttt tgaagaaatg
    21421 gaatttgcta agaaagcaaa cttttcacca aaagaagttg tacttcccat aaatcaagta
    21481 ttgttttatt ttactgaaaa agagtttaag gaaagcgatt tagacaacaa gaaaatttta
    21541 attttcttaa gagcttgtga cataaatggt ataaaaagac ttgatgaaat atatctaaat
    21601 aatggtgaag aaaaagatta tttttataaa aatatcagag ataaagtaaa gtttgcactt
    21661 gtagggtgta aggaaagttt tagaaactgt ttttgtgtga gcatggattc taacaaaact
    21721 gataattatt caattggatt aaatatagaa ggagatacac tgtacttaga tataaaggat
    21781 agtgaatttg aagtatttaa tggagaagca actgaattta atgttgatta tgtaactgat
    21841 aatttaatat ctgtagatgt gccagagaat attgattcaa atgaaattat aggaaattca
    21901 atatgggatg aatatgatac aagatgtata ggatgtggta gatgtaattt tgtatgtcca
    21961 acatgttcat gttttacaat gcaggatatt ttctataagg aaaacgaaaa tgtaggtgag
    22021 agaagaaggg tttgggcttc ttgtcaagta gatggatata cagatatagc aggtggaaat
    22081 tcttttagaa aaaaacaagg tgaaagaatg agatttaaaa ctatgcataa aatacatgat
    22141 tttaaaaaaa gattcggata tcatatgtgt gttggttgtg gaagatgtga tgatgcctgt
    22201 ccgcaataca tatcattttc aaagtgtata gaaaaaataa atgatttagt aattagtaag
    22261 gaggaagtat aaatgaaccc atatatacca gtaagtgcaa aaatactaga tatagttaaa
    22321 catactgata aagaatggac ttttagagta aactgcaata caaaaggtgt attgcctgga
    22381 aagttttatg agatatctat acctaaatac ggtgaaagtc caatatcagt atcaggatat
    22441 ggagaggatt atattgactt tacaataaga aatgtaggaa aagtaactag cgagttgttt
    22501 aattataaag aaggagattc tttttttata agaggtcctt atggaaatgg atttgatgta
    22561 tccctttatg aaggaagaga aattgtagta gtggcaggag gaagtgcatt agcccctgta
    22621 agaggaatag ttgaatattt ttataataat aaagaaaaat gcgaaaagtt taaattaata
    22681 gtaggattta aatcaccaaa agatatttta tttgcagatg atttaaagag atggagtaaa
    22741 aaacttgata ttttagttac tgtagatgga gcagaggaag gatatagtgg taatgtagga
    22801 ttagtaacaa aatacatacc agaattggat atgaaagata taaacaatac ttctatagtt
    22861 gtagttggac ctccaatgat gatgaaattt acagtagaag aatttttaaa aagagattta
    22921 gatgaaaaga acatttgggt ttcttatgaa agaaaaatgt gttgtggaat aggtaaatgt
    22981 ggacattgta aaatggatga tacttacata tgtttagatg gaccagtatt tgattattca
    23041 tatgctaaaa atcttattga ttagggggcg aagtttgtga taagagattt aaataccaaa
    23101 aaagtaatga aaaatgctta tagaattaca aaaacaaaat atgagacttc tttaagagtt
    23161 agagtacctg gtggtcttat tgaccctgag agtttagctg tagtttctaa aattgcaagt
    23221 gagtacggaa atggacaggt gcacataact acaagacagg ggtttgaaat attaggaata
    23281 aatatggaag atatggaaga aataaataaa attatacaac ctgttattga aaaaatgaat
    23341 ataaatcaaa gtgagaaaaa cgcaggttat ccagcagcag gtacaagaaa tatagcagct
    23401 tgtataggaa ataaagtctg tccaaaggct caatataata caactgaatt tgctaaaaga
    23461 atagaaaaag ctatatttcc aaatgattta cattttaaag tggctttaac tggttgtcca
    23521 aatgattgta taaaagctag aatgaatgac tttggaatta ttggtatggc tttaccaata
    23581 tatgaaaaag atagatgtgt aaattgtgga gcatgtgtaa aaaaatgttc taaaatatct
    23641 gttggagcat taaaaacaga aaataataag gtggtaagag ataaagataa gtgtatagga
    23701 tgtggagagt gtgtactaaa ttgcccaaca aatgcatgga ctagagatga gaaaaaatac
    23761 tatagattag ctataatggg tagaacagga aagaaaaacc ctagactagc agaagatttt
    23821 cttttatggg tagatgagga cagcataata aagataatac ttaatactta tagatatgta
    23881 gaaaagtata tagataaaga tgcaccagga ggaaaagaac atataggata tataattgat
    23941 agaacaggat ttatggaatt taaaaaatgg gcacttgaag gtgtagagct tccagaaata
    24001 actaaaatgt acgaaaacat atactggagt ggaattaaat atctataaat tgagtaaaac
    24061 atatatttga ataaattaaa atttaataga aggaagaggg aattatgcat aaagaaactt
    24121 tggataaatt aactaatgca gctataaata agataaattt attaaacaca agtaaagtaa
    24181 aatatttggt atcttctgcg tttgcaggac tttatgtagg tataggtata cttttgatat
    24241 ttactatagg aggacttttg actgatgcag gttcacctat gactaagata gtaatggggt
    24301 tatcatttgc aatagcacta agtttggtta taatgacggg aacagagtta tttactggaa
    24361 acaatatggt tatgtctgct ggtatgttaa ataaaggtgt aagtataaaa gatacttcta
    24421 aaatatgggc atacagttgg gtaggtaatt taatcggagc tttggtttta ggtcttatat
    24481 ttgtaggaac tggtctagta gacaaaggac ctgtagctga attttttgca aatacagcag
    24541 ctagtaaagc atccatgcca tttacagctc ttttctttag agggatttta tgtaatattc
    24601 tagtatgtgt atcagtgttg tgttcgttta gaactaatag tgatactgca aaaataatta
    24661 tgatattctt atgtttattt gcttttataa catcaggttt tgagcatagt gtagcaaata
    24721 tgacaattta tagtgtttca ttattttcac caacaataag tacagttact attggaggag
    24781 ctatttataa tttagtagct gtaacacttg gaaatattgt tggaggagct ttatttatgg
    24841 gtcttggaac ttatatatta ggtaaagaaa agcttaatta aatttattgt ataaattata
    24901 actctcttga taaatacatt tttagtatta ttgagagagt tatttttata tttttgaaaa
    24961 aatatttgaa acaaaaaaaa tatgggagca tacgaaaata tatcggttat atataaaaaa
    25021 aatacgttta tatatgaatt atatagttct aaatataaaa aaagttaaag tcctaccgta
    25081 aaaaagcgca ttttgaataa gataacaaat atgataaaat tggtcttgtt gaaattttta
    25141 ggggggatat gatgacaaag aaactagtaa tatcaaaaac aaagttgttt atttttataa
    25201 tagcatttat ttctatattt atatatttat ttggaagtaa aaatacactt ataggtgtag
    25261 gtatagtaac agctatgcta acactcttgg aaagagattt aactatttct ccaattaaaa
    25321 atttactgaa gtatttagca ataaatataa tcttaggaat actatcattt tttgcagtac
    25381 aaaatatgta tttaggagtt ttattaaatt ttatagcatt atttataata gggtatgtat
    25441 ttagttatga cttaaagaga gcagtatatg taccttttgg tctaatgtat atatttatgg
    25501 taagtatacc tgtaggaaaa agtgaatttc ctatgagatt gtcggcttta gctgttggag
    25561 cagttataat aatgattgct caatttgtta tgaatagaaa taggatgaaa aatgtcggag
    25621 ataaagaact gatatctata tgtgatgaat tattagaaaa aattagtctt ttaaaaaata
    25681 ctattaatga tgatagttct ataattaaat ctagtatgag aaaaatagat agctgtaatt
    25741 atagaataaa ttctatatct aaaaatttga aaatggttat ttttgataat agaaaagatg
    25801 acttttatat cagtattaga ggtatagaca taatgaatat tttattttca ttagaaagaa
    25861 taagtttaat catggaaaga tacaaaaagg atagtaaaga atttgaagat gaagatataa
    25921 aaaatatttt agaagaaagt agcataaata gtaaatcaga tgttctagtt gtagcaacga
    25981 aagaaataaa ctatattaag atgtgtttag aaaataaaga taccataact gataaagaga
    26041 tattaggatt tagagattat gttattgctc aggatactaa aaatataaat ttaaaagaaa
    26101 tttatagtgt tttagaaaat ttatatgagt ttttagtaga ttataaaaaa gtaaaatcaa
    26161 gagacgaaaa gaaagctgaa agaaaatcaa agatacctca tgaatttaaa cgactatcaa
    26221 tatataagaa aaactttaat ttaaattcta taagattttc ttatgcagta aagattgctt
    26281 tagctacagc tgtagcgggt tttattatgg attatttcca tttaagagat ggaagatgga
    26341 taatgttaac tgtcttttca ttaacacaac cttacgcaga aaattgtata caaaggtcta
    26401 gaaaaagaat agaaggtaca tttataggag ctgtaatatt tattgtatta ttttcaataa
    26461 taaaagatag tacattaagg tcgttaatag ttcttttagc agggtatata aactcttatg
    26521 tagttgatta tagaaaatta atggtatgtg taactgtttc agctcttggt tcagcagtag
    26581 ttatgggaga cccaaatgtt ttaaccataa gcaggatttc atatgtagca ttaggagcaa
    26641 ttattgcatt aatagtaaat aagtttatat taccatatga tgctaaaaca ggctatcaac
    26701 atgttataga aatgtataaa ggaatagtta aaaatataat tgatgaagtc aataagtcta
    26761 tagaaaatgt agctgatgtt tactatataa aaaatttatt attgatacca tcacttattg
    26821 aagatagact tatgttgata aatgctatat ataaagataa acatcaagag gattttttag
    26881 aaaatcagaa attactaata agtaatatgt ataatctata tataaatgtt aataaaaata
    26941 aaataaaaga tgaagatgtt gaaaaaatat tgagagatac taattatatt tcaaattaca
    27001 atgtagataa gtatgatgaa ggaagaagtg taattttaga gagtattgta aatactaaaa
    27061 gtctaggtga caagataata tgtctaaact tactgcaaac tctaaatggt gtcaaagaaa
    27121 tgtatagaat aagtaatata actaaagttt caataaaaga agcagcttag atattaaatt
    27181 atagaatagc catatattta aaataaagta tatggctatt tgttttataa aatactaaaa
    27241 gaaaataata tataaaaaac aaaaaacata tatttatgaa ttttatatga aaatagttaa
    27301 aatattgatt tttttcattg gttgaaaaaa caatttactt aggataaact ataatagtat
    27361 tcaaatatta tgggggttag atttatgagt aaagatgtaa aggtaaagcg taataagaaa
    27421 tatgatacat ttagtataac tatggaactt gagagacctt tactttatga tgaggttgag
    27481 aacaaagatg ataaaaataa taatagaaaa tttaacataa agaataagat atttaaggga
    27541 ataggtatac tatttgtatt gattttgctt ggctttttca tatggataaa taaaacatat
    27601 gaacctcaaa agctggcaaa agaagctcta gtcagcaata gtaaagttga agtaactgta
    27661 aatgaaaaca tttcatttac accaaaagga agaaaagttt ctaaaggttt aattttgtat
    27721 ccaggagcta aagtagaaat agaatcttat gctccacttg caagaaaaat agcagaaagt
    27781 ggatatgagg ttgtaatcgt aaagatgcca cttaatctag ctatacttgg aactaataag
    27841 gcacagaaag ttatggattc ttacaacaac atagaacatt gggtaatagg aggtcattca
    27901 ttaggaggag ttgcagcatc aaattttgca cgtgataata aattaattga aggcgttgta
    27961 tttttagctt cttatccaat gggtgatgag ttgaaagaat taggaaaaaa agttatatct
    28021 atatggggga gcaaagatgg agtagtcaat tttaaaagtc ttgtagaatc aaaacaaaaa
    28081 cttcctgatg atacaactta tgttgagatt gaaggaggaa atcatgcaca atttggagac
    28141 tatggaaaac aaaagggaga taatgatgct attataagtc aagaaaaaca acttaatatt
    28201 actgccaata gtatcattaa atttttaaag aatatatctt aaactaaaat ttaaaaaata
    28261 taattttata agttattatt taaagtaaat attaataata taatagaatg aatattttat
    28321 tacaaggggg attttaatat ggaatacgat atgccacttt acagaccacc aagtgaggct
    28381 tatagtctta taatacaagt cacactagga tgttctcata acaaatgtac gttttgtagt
    28441 atgtacaaat ctaaaaaatt taaaataaag ccactagaag taataaaaaa tgaaattgat
    28501 atatttagaa gatattataa gcatgtagaa agaatattct tagcagatgg agatgcattg
    28561 ataataccaa tggagaaatt aagagatatt attttatata taaaagaagt ttttccagag
    28621 tgtgaaagaa ttactatgta cggctctcct aaatctatag agaaaaaatc tgatgatgaa
    28681 cttaaagaat taagaaaact tggtgttaaa atgatttatc tagggcttga aagtggaagc
    28741 aatgaagtat tagaagatat taagaaaggt tttagtagtg aacaattaat aaaagttggt
    28801 agaaaagtta aaaaaagtgg gattaaatta tcagccactg taatagcagg tcttggagga
    28861 actaaaaaaa cacatcagca tgcagttgat acaggaaaaa tgttaggagc aatttcacca
    28921 gattatgtgg gtgtacttag tttaatggta gaacctaata ctgaacttta cgatttactt
    28981 aaggcaggag agtttacaat actagaggat aaggctgtgt tacaagaaat taaagagatg
    29041 ataaagaata tagatgctaa tgaaaaaatt gtatttagaa gtaatcatgc atcaaattat
    29101 gctaatttaa aaggtgtatt acctgatgat aaacaacgtc taattgatga aattgattac
    29161 tatctaagta accaaaagct aaaaagagaa gaatatagaa gactatagtt tttgatatat
    29221 caatctatat tgataggtaa atactaagat tatttagata caaaaaatca tgctacatta
    29281 atagttgctt actactgatg tagcatgatt tttttattaa aaaacaaaat ggtata
//
LOCUS       DAELXX010000043        28509 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.043, whole genome shotgun sequence.
ACCESSION   DAELXX010000043 DAELXX010000000
VERSION     DAELXX010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28509
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.043"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            175..1539
                     /locus_tag="KPH29_001806"
     CDS             175..1539
                     /locus_tag="KPH29_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBG1467406.1"
                     /translation="MDIKELVKMQRKYYNTGKTRDISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSNFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLAAGNCVILKPSEYSVSTSSVVKDIVENTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNPC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMIPAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVL
                     KKIM"
     gene            1678..2844
                     /locus_tag="KPH29_001807"
     CDS             1678..2844
                     /locus_tag="KPH29_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG1467407.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            2961..3743
                     /locus_tag="KPH29_001808"
     CDS             2961..3743
                     /locus_tag="KPH29_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBG1467408.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYAESLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(3862..4515)
                     /locus_tag="KPH29_001809"
     CDS             complement(3862..4515)
                     /locus_tag="KPH29_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG1467409.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(4539..5903)
                     /locus_tag="KPH29_001810"
     CDS             complement(4539..5903)
                     /locus_tag="KPH29_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBG1467410.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
     gene            6561..7991
                     /locus_tag="KPH29_001811"
     CDS             6561..7991
                     /locus_tag="KPH29_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG1467411.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            8094..9197
                     /locus_tag="KPH29_001812"
     CDS             8094..9197
                     /locus_tag="KPH29_001812"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBG1467412.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMTYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENKKFVAVLKEILNIKGEPSIG"
     gene            9212..9448
                     /locus_tag="KPH29_001813"
     CDS             9212..9448
                     /locus_tag="KPH29_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG1467413.1"
                     /translation="MKRGVDMNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAH
                     VEVDESKCILCGICYQVCPDNVFEIIKEKACQEA"
     gene            9465..10514
                     /gene="vorB"
                     /locus_tag="KPH29_001814"
     CDS             9465..10514
                     /gene="vorB"
                     /locus_tag="KPH29_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBG1467414.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVMEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALASKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            10516..11256
                     /locus_tag="KPH29_001815"
     CDS             10516..11256
                     /locus_tag="KPH29_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBG1467415.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            11259..11798
                     /locus_tag="KPH29_001816"
     CDS             11259..11798
                     /locus_tag="KPH29_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBG1467416.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLLEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            11922..14021
                     /locus_tag="KPH29_001817"
     CDS             11922..14021
                     /locus_tag="KPH29_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HBG1467417.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKMIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWALDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMKKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNTKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIKNRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPMLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(14288..14803)
                     /locus_tag="KPH29_001818"
     CDS             complement(14288..14803)
                     /locus_tag="KPH29_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HBG1467418.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(14895..16106)
                     /locus_tag="KPH29_001819"
     CDS             complement(14895..16106)
                     /locus_tag="KPH29_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBG1467419.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKCLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNVSNLNIVDFIKEMSDFPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
     gene            16782..18224
                     /locus_tag="KPH29_001820"
     CDS             16782..18224
                     /locus_tag="KPH29_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG1467420.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            18266..18619
                     /locus_tag="KPH29_001821"
     CDS             18266..18619
                     /locus_tag="KPH29_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467421.1"
                     /translation="MKKSNIFSIIFPTIIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKLYYKIKTFKN"
     gene            18810..19631
                     /locus_tag="KPH29_001822"
     CDS             18810..19631
                     /locus_tag="KPH29_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBG1467422.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKAYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIKKVKSNNIA"
     gene            19889..21772
                     /locus_tag="KPH29_001823"
     CDS             19889..21772
                     /locus_tag="KPH29_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBG1467423.1"
                     /translation="MFNKNKENITLSNQIKGTIQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIVMSKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRN
                     IKIMLDAVFNHIGYYSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMEWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKNIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
     gene            21996..22676
                     /gene="pgmB"
                     /locus_tag="KPH29_001824"
     CDS             21996..22676
                     /gene="pgmB"
                     /locus_tag="KPH29_001824"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBG1467424.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            22673..24958
                     /locus_tag="KPH29_001825"
     CDS             22673..24958
                     /locus_tag="KPH29_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBG1467425.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFYRELDMKNGILNRRFIVNINDKKFEVRVTRFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENAFNTPVFTVG
                     SMMYIKTNGDVEAVSHESDYNYCENSITMDVLKNKVASIEKYVSITSTRDYKESELLR
                     KCEDILNIELSKSYDDILNEHTKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNCIAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEVEFWKIISNKMYLPYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGK
                     PIKIIVYGKKYILEKYIKIDK"
     gene            25184..26077
                     /locus_tag="KPH29_001826"
     CDS             25184..26077
                     /locus_tag="KPH29_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBG1467426.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDIL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIAEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYNNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEV"
     gene            26125..28425
                     /locus_tag="KPH29_001827"
     CDS             26125..28425
                     /locus_tag="KPH29_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467427.1"
                     /translation="MKKIFSIFISCMVVVTTITGCSSGEVIKNTSPINSVKNLDFSFE
                     VKPEIFEVSVESGGSKEKISEPSKERKVSNFKKNKTEASWTYPDENIDINIKKEDSYL
                     DVNIKSNLEKETSFNWPKVEGESYILPLYEGKFIPSNDKYWKEYLNESTYNVIESFSM
                     QFFAVNKAKYSVIYIIENEFNNVIEFSSKDKINFTFKHEYPSINKNKEYGFKIYLTNK
                     NITDVAKTYKNYIVDKGEFKTLKEKSKENKNIEKLYGAPHIYFGDKSIITINDIEWNK
                     LKENLSDDLTNWMKELLSMKSGNSEFIKVINQIKTQDYIDKYQKNQIVQAFNEVMQMN
                     TFYNEKVFESKDREIKKVLDKGISKLSRVELIEFNKKLLKSKLEESVNDVATWAKYNT
                     TDVINDINKSGIKNAWIGFDDMEEGYISLEMVKRANELGYLVGPYDSYHSIHKPGEEK
                     WSTASFKDKSLYENATIENKKGEKETGFQGEGRKLNPTLSLPSVKERVQSILDEGYEF
                     NSWFIDCDATGEIYDDYSQNHITTQTQDLNARLERMSYIRDHANMVIGSEGGNDFSSS
                     TIAFAHGIETPAFAWVDKDMSKNKGSEYYVGKYYSPTGGVPEIFSKQIPVKERYKKIF
                     IDPIYSLPLFRLVYNDSVISSHQWLWGTLKIKDEVGSRMMKEVLYNTPPMYYIDRTEW
                     EKHKDEIISNNKVWSAFNKKAIKKEMTDFKFISEDRLVQMTEYGEDLKVLVNFSQKDI
                     KYNDEVIKANSLVIYDADSKVIYKPE"
BASE COUNT        11177 a         3143 c         4943 g         9246 t
ORIGIN      
        1 tttaaaataa attattttat aagcttactt atacttaata aggggctttt tttattggaa
       61 gatttatgtt atattgagat gaatttatta tgatataaaa aataatgtta ggataaattt
      121 aaatcttttt ctaaaaatgt attaatataa ttcttaaaga aaggattttt aacaatggat
      181 ataaaagagt tagttaaaat gcaaagaaaa tattataata caggtaaaac aagagatata
      241 tcgtttcgta tagaacagtt gaagaaactt aagttagttg tatcacaaaa tgaagaaaaa
      301 atacttttag cactaaagaa agatttaaat aaatcgaatt ttgaaggttt tatgacagag
      361 gtaggcatgt tttatagtga attaaacttt gctataaaga atataagaaa atggagcaaa
      421 ataaaaagag ttaaatcaag tatggttaat tttccatcta tatccaaagt tgttccacaa
      481 ccttatggtg ttactctcat aatgtcacct tggaattatc cctttcaatt agcacttata
      541 ccattagttt ggtcattagc agcagggaat tgtgttatat taaaaccttc tgaatattct
      601 gtgtcaacat ctagtgtagt taaagacata gttgaaaata ctttttcaaa agaatatgtt
      661 gcagttgttc aaggaagtca agaagaaagt gaaaagttac tattggaaag atttgattat
      721 atatttttta caggaagtac taatgttggt aaaataatca tgaaatcagc atcagagcat
      781 ttaactccta ttactttaga acttggagga aaaagtcctt gtatcatttt aaaagatgct
      841 aatatagacc ttactgccaa aagacttact tggggaaaac taataaatgc aggacaaacc
      901 tgcgtggctc cagattatgt tttagtgcat gaagatagaa aaaatgagtt aatagagaaa
      961 ataaaatatt atactaataa gtattttgga gataatccat gtaataatga gcaatttcct
     1021 aaaatcataa atcagagaca ttttaatcgg atattatctc ttattgataa agataagata
     1081 gtctatggtg gaaattataa taaggagaca ttaaagatag agccaactat agtagacaat
     1141 gttaattggg atgataatat aatgaaggaa gaaatatttg ggcctatatt tcccatttta
     1201 acatataaag atttagatga agtaatacaa aaaattatac aaatgccaaa tccattggca
     1261 ctttatattt ttacaaaaaa taagtaccta gaaaataaac ttttagaaat gattccagca
     1321 ggcggatgtt gtatcaatga tacagttact catatagcta cgaattactt accttttggt
     1381 ggtattggtg aaagtggtat gggctcatat catggaaaag ctggattcga tacatttact
     1441 cactataaga gtgtgttaaa aaaattaaat ttggatgtcc ctattagata tgctccatac
     1501 gataataaat taattaaggt tcttaaaaag ataatgtagg ttatattgta gaataataaa
     1561 tataaataaa attgagtatt tgtattctga atatatttta tttttaattt ggtatatttg
     1621 ttataaaagt ggtgattttg tgataaaatt taattatata gaaataagga ggaaaaaatg
     1681 aaaattaatt ggaatacaaa atacacaaca atagcaattt atacatttat aatagctgct
     1741 agtagtatta tattttatct agtatcatca caaattgatg tattttctaa taatttagat
     1801 gctatattta caacattaca accttttata ataggatttg ctattgcata tcttcttaat
     1861 tttattttaa agttttatga agacagaatt tttataaaaa gtgagaaatt aaaaaaacta
     1921 aaacaaagtt caaaaagagg cttgggtctt ttattaacat atgcaacagc tacattgata
     1981 ctttacttat ttatgcattt cgtattgcca caagtaattg agagtattgt tggactagca
     2041 aatgatatac ctatgtatgt aaacaatgca actaagctta ttgataaatt aatgactgat
     2101 ttaaatcttg atgagcaata ctttaatttg gcagttgata agtggaatga gtttgttaca
     2161 tatattataa agtttgtaac agatttaatc ccaatattag gaagtatgtt aaagaatgta
     2221 gcctctagta tatggaatat tgtactagga ttaattgttt cagtatactt attaattgat
     2281 aaagaaaaat tctatggatt aagcaaaaaa ataacttatg cagtatttac agaaaaacaa
     2341 gctgccagaa ttttagaatt aactcataga agtaactata catttggaag atttttaggt
     2401 ggcaaaatat tagattcatt cattataggt attctaacat ttgtaatact tacattagtt
     2461 aaaatgccat atacattatt aatatcagtt ataattggta ttacaaatat tatacctttc
     2521 tttggacctt tatttggagc tataccatct actctaatag tgatgtttgt atctcctata
     2581 aaagctttct ggttattgtt gattatatta ataatacaac agatagatgg aaatattata
     2641 ggacctaaaa tacttggaga ttcaattggt atatctgcat tttggatttt gttctcctta
     2701 ttggttgcag gaaaattatt aggttttatt ggaatggtta taggagttcc aatgtttgca
     2761 gtaatttatt ctataattaa ggatattgtt gaaagtaaat tggacaaaaa gggattaccg
     2821 acagatactt ccgattacat gtagctaaaa aatattatgt ataattaaaa atatataaat
     2881 taagcaatat ttaaagcact gagaaagtta aactcttggt gctttttaga tataaaatta
     2941 ttatagaaga ggtgtttttt gtggcattta tagaatgtaa atttaaatct aaatatactg
     3001 gaggagaaac tgatataatt gtaattttgc ctgtagcaaa tggaaaagaa ctttttagtg
     3061 gtaaagagat atatgatttt gaaggtaaat ttaaaacatt atacttattt catggtttag
     3121 aagatgacca aagctcatgg attagatata ctaatgtaga gcattatgct aaaaataaaa
     3181 atatagccct agtaatacca agagtagaaa ctagttttta tacagatatg gcgtatggac
     3241 accaatattt tacttttgta agcgaggaat tacctaaatt tgttagagct atatttcctt
     3301 tatcagataa aagggaagat aactttgtag ctggtatgtc tatgggggga tatggagctt
     3361 taaaattagc actttcaaaa ccaaaagagt tttcggcagt tgcaagtttt tctggttcgc
     3421 ctgatatgat tcatgagtta gaaaatcaac aaatagaaac caatgcagac attttatttc
     3481 acgattttgg tagaccagaa gacgctagaa atagtgagaa tgatttactt tacatactta
     3541 agaaccttaa agaaagaaat gaagatattc ctaaaattta tcaattttgt ggtgatagag
     3601 attttttata taaaaataat cagatgttta aaaaatatgc agagtctctg ggtgtagatg
     3661 ttgaatatga agagtgtgta ggggggcatg aatggcgtct ttggaattta tggataaaga
     3721 aatttataga tataattggg tagtacactt atggtattaa caggtatact ataaaatatc
     3781 tttaaatatg ataaagactg aaatcctaaa atcaaattat gattttaaaa tttcagtcta
     3841 taagaattta ataaaatttt attaagttct tacacgagtt attattggag taaaattgtc
     3901 aactatatct atatagtatt tactcaattc ctctaaaatc tcttcgcata aatccgtact
     3961 atgaccaaga ttaataagaa actgttttgt aaaatgcttt attacagctt gagaagatat
     4021 atcgtacttg tgtgaagtat aaactctaat tatttcaggt ttcatagcaa gtataccaat
     4081 acaagaaagt attaattcat catgttctat agattcttta tcattaacat atcttttcat
     4141 gaccatttcg gttacacttc tttgatagct taaaatagct tcttcataag ctatatccaa
     4201 ataaaaattt ctatatgctt cgttttttag aaggaaacaa aggtcagcat tcatagcaag
     4261 aaagaacatc atcatagtac cttcttcatg gtagttatag attaaagact gcatttcatt
     4321 tcttatatct tcatatatta tagctcctat ttcgctttta cttgagaagt aatagcttat
     4381 tagacctaaa tttatattag ctctttctgc aatttgccta gttgttgtct tatgataacc
     4441 ttgctcaaaa aataattctc tagcagcact tataatacta gcttttgttt ctttgccctt
     4501 ttctgatttt gacatattta ctgtgtatac ctctcttttc aatctttttt tgaaaatttg
     4561 ctaactataa atattgtaat agaaccacct attataggaa ccaaaattct gtcaaataca
     4621 gtaagaatac caatcatatt taataatgcc aatataacac ttactataac agcatagata
     4681 gctccttttt ttgtagcgcc tttccagcaa agaccaatta caaatggtgc aaatattaca
     4741 cctgtacata atggagccgg ccaaaacaat aattctaata aactatcaaa tttaagtgct
     4801 atgattgcac ctattatagc tgtcaaagct gatgcaattt gagaatactt tagagtattt
     4861 tttaaatcat taaattctat attaggatgt aataatttgt tataaatatc tcttgatata
     4921 atagcactca ttgaggtcat taatgaatcg gcagtagaca ttatggcagc caatatagca
     4981 gctaagaata atacagatat taaaggtggc aaagtagtcg atatcatttg aggtaaaacc
     5041 tgaacagatg atatgtttgg aaataaacta ccaccaatag ctccaattat tcctataggg
     5101 aaggcaagaa ataatagtat aaatcctgca attacactac ttttaactgc atttttagca
     5161 ttatcagcac attgatatct taaaaaactt tcataagaaa atattgagcc taaaactaat
     5221 ggtaaaactg tatacataaa atcactaatc gatttatttc ctggaaagaa tggttctttt
     5281 accattgatt gtaacataag taaagggttg tcgccaacta attttaagct tatataagct
     5341 actattggca aaaacactat acaaatagca agttgaataa atgctgatac tgcaactccc
     5401 cacattcctg atgtataagt atatataatt ataactataa ctcctataat tacagctata
     5461 tcataaggaa taccaaatgc agtagataca gaaccaaaag aaattaattg actacccata
     5521 atagctagta tagatattgg tgttacaaga ctttgataag ctcttaaaaa tacactatga
     5581 ccatatctag tttcaatata atcagcaggt gttatatcac aagataagct tcttaatttt
     5641 cctgcgaaaa aggcatttat aaagcaagct aaagctgcac ctatagtaga atacattcca
     5701 acccaaagtc ctctttcagc tccccattga actccaccaa ctaagcaccc tcctccaaaa
     5761 gatgtagcag caatggttgc agctagtata ggcaaaggaa gatttctacc tgcgattagg
     5821 aaatcggatg ctgttttaac tttttttgaa aaataaaaac caatccatat gtatattgca
     5881 cacgtaacaa ttaaaagtaa catagtcttt tctcctttat aaaatatttt aataaaatat
     5941 ataaaatata tttaaaatat attttatata aaagtagtac gatatttgtt ttattggttt
     6001 tagattaata tagctataat aatttatgat attagatgga aattacgtaa aaatagaaaa
     6061 ttgaaaattc tgaaattttt gcatattgat atttatctca tagattcttt tttgtaattg
     6121 taacacgctt ttatttttaa atcaatagta catgtataaa aataaaagga atctaaataa
     6181 gaaaaagata tttttaatat ctggatgtag tatagaaata tcttgaataa attattcttg
     6241 aataaaatta taaaaattta aaatcactag aaatatgata aaataattta aaatacgcat
     6301 ttcaatgaca tttcaagaca agaaaataat aaaaaacaaa tctaaattaa gcctaaatag
     6361 ctttttagtt aattttagta gtaaaaaata aaacttaaaa agtgtttgac aattatgaat
     6421 tttaagaata taattaaatt aaaattatac acatataaaa atatactcct gaatcaagaa
     6481 tgagatattt aagattcaaa atatatatta ttatacgtga ataatgaaaa gtaagaaaat
     6541 tttataaggg aggggtagat gtgagtagtc aagaaagcaa accaaaagga aatcttatat
     6601 caaattttag agatgatatg agagacccta agaaaagagc aatatacaca tattcaagtt
     6661 ctataaactt agcgtacaat tttataggtt actttgtatc aactgttaca aatatttttt
     6721 taactgatgt ggcaaaactt ggtgtaatta tggcaggtgg tatgcaaatt atacaatctt
     6781 tcataaaaat tgctgtagct cctataacag gtacagtatt tgacaaacaa ccattcaaaa
     6841 aaggaaagta ttatccatgg cttagatatg caccagcagc gttagcatta acatatggaa
     6901 tatttttcat agttccatta atgaaaataa gtccagcatt acttttacct ttgatgacag
     6961 ctttattatt aatagcatct tgtgctcagc aagtagtttt cactataatt tatgctattt
     7021 accctgtagt tgctaagaca cctagagacc gagttatagg ctcaactact actaatatat
     7081 ttaaagaagt tggtaaattc ttagtaggtt tttcatatcc gttattatta gttttatttg
     7141 cagatgtatt aggtggagaa ggaatgggtt atttaggtac ctacttattc tttgcagcag
     7201 gatgcctttt agtattttgg ttttcttcaa cagagataat aaaatctgga gcagaagaag
     7261 aagttcttca aaaagaagag aagccaaaga ttaaagcaag tgaaatggca aaagctctat
     7321 tcactaatgt accacttatg atggcatttt gtttagaatt tcttatatgt ataagatcta
     7381 taggattagg acctttggct ccatattatt ttaaatatgt aatagaagat gaacgtggat
     7441 tagcagtatt tttatcaatc atgcctttgg taagtgtagc attcatgttt ttcgcaccag
     7501 tgtttataaa aatctgtaga gagcaaaaac tagcttcaat aatttctttt tctatatgtg
     7561 cactatgcca cttattggtt gcatggactc cttgggggaa aactacttta ggagtaacta
     7621 tgttgctggc aattggtgga ggtttttcaa atgtagtatc aataataaat cttaatttct
     7681 ttgccggttc ttgtgattat gggcattgga aatcaggaaa agacttacct ggcttaagta
     7741 tgtcactata tccagttgca atacaagtag gagtattact tgcaactact ataagaacag
     7801 ttttaatgaa ctcaatggga tatcaagctg atatggtagt cacagaagca gttaaatcag
     7861 gttttataaa tatgatttct tattctatgg caataccttt aataatagct gttgttatag
     7921 caatactata tccagtaagt gacaagaaat taaaccaaat aagagaagag ttaaatcaaa
     7981 gaaatgctta gttaaaagaa agttaagtag atggttaatt taagctatta aagtaagtag
     8041 acgcaatagc gtttatataa atcaaaatac atatacagag ggggctttta aaaatgatag
     8101 aaaaattatt gagaaatacc gtaaaagaat tacatcaata tgttccaggt gaaccaatag
     8161 aaaaagttaa ggaaaaatat ggtgtaaaag aaataataaa attagcttca aatgaaaatc
     8221 ctctaggacc atcaccaaaa gcaatagaag ctatgattga aatgctaaaa caaggtcaac
     8281 tttatccaga gccagaagct aacgaactta gaagaaaatt agctgaaaag ctagatttaa
     8341 aaccagaaaa ttttattgtt gctaatggtg cggataacgt tatcactcta ataggtgagg
     8401 cttttataaa tagaggagat gaagttatat attgtaaccc aacatttcca tcatatagaa
     8461 ctgcaactat aaaaaatgaa gggataccag tagaagtgcc tttaacagaa gattataagt
     8521 atgatttaca aggtatatta gataagatta cagataaaac aaaattaata tgtgtatgta
     8581 accctaataa tccaacagga acaattgtgg atgataaaga gttagaagaa tttttaaaga
     8641 aagtccctga aaatattata actatattgg atgaagcata tatagaattt ttaacagttc
     8701 ctaattatgt ggatggactt aagtatgtaa gggaaaatta taacgttata gtaacaagaa
     8761 ctttctctaa aatatatggt cttgcagggc ttagagtagg atatggtatt gctaaagatg
     8821 agataataag aactttattt acagtaaaag agcctttctc tgcaaatcgt gttgctataa
     8881 gtggagcaac agcagcactt gatgatgaag agtttataaa agaaacatat gaattaaata
     8941 gagttggaat gacttacttt aaagaagagt ttacaaaaat ggggtttgat gttgtagatt
     9001 cacaatcaaa tttcctttat gtagatatga aaacagatat acctaagtta tttgaagact
     9061 tgaaaaaaag agggtttgta ataagaccaa gtgctacaca tgcacgtgta agtattggaa
     9121 ctatggaaga aaataagaaa tttgtagcag ttttaaagga aatattaaat attaaaggag
     9181 agccaagtat tggataatag atagaaatat tatgaaacgg ggtgtagata tgaacttaaa
     9241 aataaacgca gagagatgta aaagttgtga atactgcgtt atatcttgta aaaaaggagc
     9301 attaaaaata tcaagtaaga taaataaaga aggttatgct catgtagaag ttgatgagag
     9361 taaatgcata ctttgtggaa tttgctatca ggtgtgtcca gataatgttt ttgaaataat
     9421 taaagaaaaa gcatgtcaag aggcttagaa ggagttggga taatatgaaa aagatgtgga
     9481 aggggaacca cgccattgca gaagcagccc taagaggagg ttgtgaattt tatgcaggat
     9541 atccaattac accacaaaca gaggtaatgg aatttttatc acatagaatg tctgaacttg
     9601 gaagaacttt tatacagtcc gaaaatgaga tggctgctat atatatggta tatggagcat
     9661 atgcaagtgg aatgaggtct atgacgagtt cttcaggacc aggaatatct ttaaaacaag
     9721 aagggatttc ttacttatgt gcaaatcatt acccttgtgt aatcgtaaat gtacagagat
     9781 gggggcctgg tcttggctca ttggactcag cacagacaga ctatctaaga gatactagag
     9841 ggggaggaaa tggagattat catttgatag tttatgcacc aaacagtatt caagagactg
     9901 tagatttgat gtataactca tttgatgtag cggaaaaata cagagtgcct gtcgaaattt
     9961 tgacagaagc agctttaggt caaatgatgg aaccagtaga atttccagaa tttaagaaga
    10021 gagaaggaga tttaggttgg acttatgatg gaagtaatag agaccatgca aaggttgcag
    10081 ataaccaaaa gccaactttt tgtatggaga aacgtatgag aataactgaa aatgaacaac
    10141 aatgggaaga ttatcaagta gaagatgcag aatatgtgtt tgtagcgttc ggtataccat
    10201 caagaacaac tatgaatgct gttagaagac ttagagaaca aggtgaaaaa gttggaataa
    10261 taagaccaat aacagtatgg cctttcccat ataaagcatt tgaaaaggta agtaaagatg
    10321 taaaaggatt tatctcagta gaatcaacag acacaggtca attagtagaa gatgttgctt
    10381 tagcctcaaa gaaagtatgt aaagaaaatg tacctgtgta tggtttattt agtggaaatc
    10441 atataccaaa aacattgcaa gtaatggaca cttatagcaa aataaaatct ggcgaaatca
    10501 aggaggtgtt ctaatatggc tacagaaaaa ttaacaccta gcgttgtttc aataccaaac
    10561 aagttttgcc caggttgtgg tcatggaata gtaaatcgta taatagcaga ggtaatagaa
    10621 gaacatggat atgaaaaaaa tcatgtatta acattaggag ttggttgtgt ctgcaatatg
    10681 aattttagct ggaatggtga taagatgcaa actgcacatg gtagagcttc atctacagct
    10741 ataggagtta aagtagcact tccagataca ttagtaatga cttaccaagg tgatggagat
    10801 gcttatgtta taggtctttc tgaaacttta aatacagcat atagaaatca taatgtgact
    10861 gtatttgtaa taaacaataa taattttgca atgactggtg gacaaatgtc atggaccact
    10921 atgccgggtc aagtcacaac aacaagtgta aatggaagag atataaagac tactggtagt
    10981 ccactgaaag ttcctgagat ggtggcaaac ttctttgatg tagcatatgt agcaagaggt
    11041 tcagttcata gcccaaagga aataattaat ctaaaaaaat atataaaaaa tgcaatagaa
    11101 gctcaattaa atggagaggg atattcctta gttgaaatat tagcaccttg cccaactaac
    11161 tggggtgtat cactagagaa atctatcaaa tggatggaag aagaaatagt gccatattat
    11221 gctcttggcg aatttaagca aagggatggt gaatagttat ggaaaaagag ctagtatttg
    11281 cagggtttgg tggtcaagga gttttaactc ttggtctgat tgtagctgag tcagcacttg
    11341 aattggggaa acaagtaact tggatgcctg catatggtcc aactatgaga ggtggaaaag
    11401 cctatagtgt agtaaagttt tctgatgaga gtataggtgg gcctgatatg gaagaaatag
    11461 atgttttagt agcaatgaat aaaccatctt tagattatat aaatttagtt aaagaaggtg
    11521 gaacagtagt tattaataca tctgcaatag atgagaatgt aacattaaga gaagatataa
    11581 gtgtagttaa aataaattgt caggaattag ctcaaaaagt aaacaatcca aaagcagcaa
    11641 atatagtagt acttggagca ttaattgcta aaactgggtt acttgaaaaa gaattagctt
    11701 taaaaactat gtgtgatttc tttgaggaaa aaggaaaagg aaagtttaat gttcaaaatg
    11761 aagcagcatt catggaaggc tataataacg ccatttgata aattaccctt atcttaagtg
    11821 atttatctca agatttaata atctttagtg ccaagagaaa cttcatcttg agataaaatt
    11881 cacttaaaat cacaactata aacatatagg agcgtgaatt tatgagtttg gaaaaactta
    11941 ttagaccaaa aagtattgct atagtaggag tgactgataa acttggtttt ggaagaagtg
    12001 cagcacttag tatagtaaaa agtaaggaaa cagatagagt atattatgtt aatcccaaaa
    12061 gagaagaatt gtttgggaga aaatgttata agatgataca agaagttcca gaagtagtag
    12121 actgtgtagt agtatgtacg cctagaaatg tagttccatc tgtattaaaa gattctggag
    12181 agttaggaac aaaggcagca gtggtatatg ctagtggatt tgcagaggaa ggtacagagg
    12241 aaggtacaga tttagaaaat caacttatag aaatatcaaa tacatacgat atgaaaatat
    12301 tgggtccaaa ctgcatgggg ttgctaaatt gtatagataa ggttaatcta tgggcaggag
    12361 gttctaaatg ggctttagat actaaaaaac ctggtatagg aattgtaggt caaagtggat
    12421 ttataactgc tgaaattgtt tcaagtgatt attttaatat atcttatggt ttttcaacag
    12481 gaaatggaaa tatagttaca ctagaagatg cagtagattt ccttgtagat gatgattatg
    12541 catcagtaat agcaatttac cttgaaggtt taaaaaatcc tcaaaaattt atagatgctt
    12601 taaaaagggc agcacaaaaa agaaagccag taataatatt gaaatcagga aggtcagaaa
    12661 aaggtgctat ttctgctgcc tctcatacag gaaatttagc tggttcgagt aaagcttttg
    12721 aaagtatttt tgaaaaatat ggagtaattt ctgtggaaaa tttagaacag tttatgtgtt
    12781 tagcacaagc ttttagtgtt ttagatggta atttacctgc aaattctaat tttgcagcaa
    12841 taaacttctc aggaggagaa aatactattt gtgcagattt agcagaagaa aatggtgtag
    12901 aacttgcaga aatatcttct gaaactaaag aagaaatgaa aaaatatctt ccaggatttg
    12961 caacacctaa aaatccacta gatgctacaa cagcattatt ccatgaaaag gatatgatag
    13021 taggtttatt acataccttt gaaaaggatg atagtgttgg atttactatg gtaggtgcaa
    13081 atataagaga tgaagaaaat gaaatgcatg aaaccctttg taaagctgta agtgaagcta
    13141 gagaacaagg attgaaaaaa cctgtgtttg cagttcctac acttgaagga aatagatatt
    13201 tagattatag gactagatta gaagataatc aagtgcctat aatgtcatca gttggaacta
    13261 catttacttg ttttaataaa atggctaagt ttatagatta tgattattca aaaagaactt
    13321 tagaatttaa agctgttaag aaaagagagt caaataatgt agtagcatta tcagaattag
    13381 atagtaagat tgagatgaaa aaatatggag taccagttcc aggacaaggt aatacaaaaa
    13441 gtattgatga attagatgaa atcttaaaat atattaagta tcctgttgtt ttaaaaataa
    13501 attcttctga aatattgcac aaatctgatg ttggaggagt taaaatagga attaagaata
    13561 gagatgaggc agtagatgca tataatgaga ttttaactaa tgtaaaaaaa gcaaaaccag
    13621 atgctaatat tgatggtata ttagttcaag aaatggtaga aagtggtata gaaattatta
    13681 ttggtataac taatgatgac caatttggac caatgttatt agtcggttta ggaggagtat
    13741 ttgtagaggt atttaaagat actacattat atccattacc tataaatcat gatgaagcta
    13801 ttatgatgct taaaaaacta aaatcattta agcttttaaa tggctataga ggaagtgagc
    13861 cttgtgatat tgatgctctt gcagatatga tggtcaaatt aggaaaatat gcttatgaaa
    13921 ataaggatga agtcaaggaa atagacttaa atccagtgtt tgtatatcct aaaggaaaag
    13981 gcgtatgtgc agtagatgcc ttaatagtta aatataaata gtttataagt tttggtattc
    14041 aaccataatt aaagcaccga ctgaaaatca cattttgtaa gttagatagt gtgattttca
    14101 gtcaccccaa atttaattta aaaatatttt aaaatgaaag tatagttttg agtttaattg
    14161 tgaaatagca ctgtataaac aataaaatac tacgtataaa acgaataata aaaggtgttt
    14221 taatgataag aatcaatctt gattttcatt aaaacacctt ctttattata ttaaaaattt
    14281 ttaaacgtta tattgtcaca ggtgggtttg ttggagctgc atatgtacga ttagcaaatt
    14341 cttggtcaac aaagaataga ccaccctctc cagcaagttt aacacgtttt atattgtctt
    14401 cacaatttgt ttcttcttct gcttgttcag atataaacca aagtaagaat tgagaagttc
    14461 taaagtctga ttcagagttt gctacagctg ctaagttgtt tattaaagct gtaacctttt
    14521 tttcatgagc taacgtacgc tcaagaatat ccattgcaga agtaaagtct gaatctggca
    14581 ttgggatagc atcaagctga acctttcctc ctacattatg taagtatttg taaaaaaaca
    14641 tagcgtggtc tgtctcttct ttatattgaa cataaaacca attagaaaat cctttaagtc
    14701 cttctgcttc aaggtaagaa gacatagaaa gataaagata cgcagaatat aattcatggt
    14761 taatctgctc attaagtaat ttttccattt tagctgaaat catttttata cctccaaatt
    14821 ttaaatgctc ttataaaata taataatatt aaatggaaaa atattcaata aaagtgttta
    14881 aatattttac atatttaata aaaatttata ctaccattaa aaaaattata acttacaatg
    14941 gctatagagc caattaatgg tgcatttgct ccaaaatttg aatgccttac acttattttt
    15001 ttatatttag aaaaacttgc tgaagcagtc aattttttta ataatgtatt ttctataaat
    15061 gtaccaggta tattaaagtc atagcctatt attatagaac tacaatcaat caatgtaagt
    15121 gtattaacta atgcatatga tacgtatgta caaaattcat caagcaaaaa catggcaaga
    15181 ctatctttat tatttgcctc atctattaac ttgactaaag aaaaatcttt acagttaatt
    15241 aatggggagt taggataaat atggctcaat gattcaattc ttttaattag attactgaca
    15301 cttgtataat aatctaaaca tccattgtta ccacaactgc acaatggtcc actaaaatta
    15361 attgatgtat gtcctatttc tccactttgt cctaggttgc cagtatgaag tttattttct
    15421 agaactagac cagctccaat accattcatt atgtgtaggt atatataatt tgatatattt
    15481 ttacctaaac catacatttt ttctgctaat gcacccgaat tagcatcatt tatcaaaaat
    15541 attggaaaat cagacatttc ttttataaaa tctactatat ttaggtttga tacatttcca
    15601 aagtctgggg gatttagtat ggtaccattt atatcatcta ctggacctat tgaagaaata
    15661 ccaattccaa ttatatctct cttgttgatt ttcattattt tagtaaacat tctttttatt
    15721 atatctttta aaacatcatt tgatagacat ttagggtaag tttcagaaag ttgttttaca
    15781 atattacctt ttaaatctga aataattacc ttacatatat ttcttcttat taggattcca
    15841 caaatacaag gcgatatatt agaaatatct aataaaatag gtttacgacc tgatgaatgt
    15901 gcatttaaag ttgatgactc tatttcccta atcatatttt cattaataag ttcagtaact
    15961 agatttgata aagtcatttt agtaagtcct gtagacttag ctaaatcaac tctggacata
    16021 ggtgtatttg tagaaagtaa tttcaatatt attaacttat ttttaatttt tacatctttg
    16081 atgttagaac cttgtttgtt actcatcata aatctcctta aataaagtta agtgttttat
    16141 ttattttcaa gtatactatc accttataat agagtaacat ttttttactt gaaaatatca
    16201 ttaattagaa tatatcacat tatatatcat aaaacatata aaaaaatgtt agaattaaat
    16261 taaaagtgaa aagtagattg atatataaat agtaaatact taattaaaaa aagataatga
    16321 tagcaataat tattgttacc aagaatctac tacttcaagt gttaccttag gtatttaagt
    16381 tgatagtggt ttgcacgtaa taaacttgat gtaatatata ttcttaataa tatcgataaa
    16441 agatttactt aaaaataata tagattatgt aaacatataa tctatttatt gtatacaata
    16501 aaatttcatt ttgtaaaaca gtgaattaaa tatgtaatat agatatcgat ttttattaca
    16561 aattgttact aaatataggt acattagaac aaaaatctag taaattgtaa ctaaaatgta
    16621 aatttttcct ttaatctata caatattatg gaaacttttt gtaatttatg atataataac
    16681 aaatatataa ttattaaatt ataataaatg actatagcgt agatgtaata ctatgttata
    16741 gtcatttttg aattaaactt gatatatcaa gggggaagaa tatgagaaca attagaagag
    16801 ttttagcatt aggtttaaca ttagctatat ttctaataaa tgtacctaat gtagatgcat
    16861 taacatcaga tacaattaaa ggtaacaata tatatgagac ggctggatta atagcagata
    16921 aaaagagtta tgatacagca attatggtaa atatggataa ttctatagca gatggtcttt
    16981 cagcaagtgg tcttgcaggt gctgttgatg ctccaatttt gcttgcacaa aaaaataaga
    17041 taccaaatga aacaaaacaa agattaaaaa atgttaaaaa gatatacata ataggtaaag
    17101 aattatcaat aagtaagtca gtagaaactg aactaaaaaa cacaggggca caggtaacta
    17161 gattgggtgg agatgacaga ataaagacta gttatagtgt tgctaaagag gtaaatggta
    17221 tcaaaaaagt tgatgaagtg atattaacta atgcatataa aggagaagca gatacaataa
    17281 gtgctgcacc cgtatcagta agagatatag ctcctatagt acttacagat ggaaagagtg
    17341 tgcctttttc aacaagcaat gtaaaaacat atgcagtagg tgggagtata tcaatgagca
    17401 ctagtttagt taataaaact aatgctaaaa gacttggtgg ttcagataga tatgatacaa
    17461 ataagaaagt tataaaagag ttttatccag atgcatcaga attttatttg agtgatggat
    17521 atgatttagt aaatgcactt acaggttcta caattgctaa ggaaaatcca attgtattgg
    17581 tgtcagaaag tagtgataag tctatattag caggagcaga taagattact agattaggtt
    17641 caataagtga cagtgtgtat aataagtgtg tttctgctgc acaaaataat ggcgattcgt
    17701 ctacaaaagg tgagtcacct atgaaaaatg agacaagcat attaggtcag ccaacagcta
    17761 gtttagaagc gtgcttaaaa tgggcaaaat ctaaaaaagc taatgattta tttatagagt
    17821 taataccaat attatatgat actgctgttc aggaaggtgt taatcctgtt ttggcagtag
    17881 ctcaatctgc aaaagaaact ggtttttgta attttggtgg agtattagat gcatcattta
    17941 agaatccttg tggacttaaa acttctgtag gtggttctga tactgataag aatgcccatt
    18001 caagatttga tacttgggaa gaaggaatat tagctcaaat tcaacattta tgcttatatg
    18061 caggacaaga tggttaccca ctttcaaatc cagtagaccc tagacatgaa aaatctttat
    18121 ttggtaaggc taaaacagtt gaaagtttat ctaataattg ggctggaggt caatatggac
    18181 aagatttagt aagaatgatg ggagaaattg aggcaacaaa ataattgaag attatcaaat
    18241 atccaataaa taaggattga ttattatgaa aaagagtaat atttttagta tcatatttcc
    18301 tacaatcata atggtgctaa tgtttttaac ttttgctaca gatatattta atattccaga
    18361 tattcaatct aaaagtattt ttgttgtggg aatggttttg atttttccaa tttctttttt
    18421 aatacaaggg ataatttgtg tattaaataa gactaattgg attttatctc ttattgtgtc
    18481 tttagtaact tatatagtat taatgtatat atttttaaat gattctgcat atatatatgt
    18541 tttactttat acagcttttt atatgattgg acatataaca gctaaactct attacaaaat
    18601 taagactttt aaaaattaaa ttgaagtata taaaagtaac attaagagaa gtatccttaa
    18661 tgattttcta aaatcagaaa taggtgcttc tcttttttgt aaaaataaaa gtatgtaaat
    18721 aaaatttaac cattataatt ttcttttaac aaatgtatac aataaacttg ttgtataatt
    18781 aagatagata aaataaaggg agggataata tgaatttaat agataatcat attcatacta
    18841 atttttcaag tgatggaaag gattccatgg aagatacaat aaaaaaagct ataagtattg
    18901 gtgttagata tttgactttt acagaccatt tagaacatga tgaagataga ggattttgca
    18961 taaattataa tgactatgtt ccagtattta ataagtttaa agaaaaatat aaaaaggata
    19021 tagagttatt actaggtgtt gaagtaggtt acagaaaaca tctaaaaaat gaaatagaag
    19081 aaataataaa ttctaatcct tttgattttg tattgtgctc aactcataca attgacaatg
    19141 tacctgttcc atcaaaggcg tattttaaag gtcttagcaa agaagatgcg tattataaat
    19201 attttaatag catacttgaa actaatcgtg aatttggaga ttacaatata tatggtcatt
    19261 tggattatat atctaggtat ggaatatatt cagataatag agttatatac aatgacttta
    19321 aagatgttat tgatgaggtc ttaaagtcaa taatcaataa tgggagtggt atagagctaa
    19381 atacttctgg ttatagatat ggattaaatg ctatacatcc aaatgaagat attctaaaaa
    19441 gatataaaga acttggagga tttatagtta ctgtaggttc tgattctcat agggtagaag
    19501 atatatgtaa agattttgat gtggcctatg atatgcttaa gtatcttgac tttaaatatg
    19561 tatctttatt taaagaaaga gaaacttatt ttgtaaatat aaaaaaagtt aaatctaaca
    19621 atatagctta gagtatgttt tgaagtaaat taatggatag ttaaaaagtt aatagttata
    19681 atattgtatc ctctagaaaa atactagagg tttttatttt gctaaaataa ctaaatagaa
    19741 ttaaatttaa atagtatagt ttattttaaa ttgaatagtt tattgttgat atattttact
    19801 attatatatt tttattgtga tataatagta aaatatatta acctatcttt agattatata
    19861 aattattata aaggagggaa attttaaaat gtttaataaa aataaagaaa atataacttt
    19921 aagtaatcaa atcaaaggaa caattcaaaa tagtttaaca aaagaagcta tattacatat
    19981 accaatgagc aattatgcct atggttatga tagagaaaca ctacatataa gacttagaac
    20041 taaaaaaaat gaagcgaaaa gagtaatttt acgaatagga gaccaatatg tgtgggataa
    20101 aggaggtgca ggaggaggaa atttaaatgc atctggagtt ggttggtctg gtggaacaaa
    20161 tatagtaatg agtaaagaag tagaaactga attatttgac tattggatag ctaagtgtaa
    20221 gcctttaaat aaacgctcac gatatggttt tataatagaa ggtcaagaag aaaaactttt
    20281 atttacagaa aaaagaataa tagagttggg aaatgaaaat gatgaaaagg aattatgtga
    20341 aataggtaat ttttttggat atccatattt aaattatata gatataccta aagtgccaaa
    20401 ttgggtgaaa gatactgttt ggtatcagat atttccagat agatttgcaa atggtgaccc
    20461 gtctataaac ccagaaggag tagataaatg gggagctatt ccaaccagag ataattttac
    20521 aggtggagat ttacaaggtg tgatagaaca cttagattat ttgagtgatt tgggaataaa
    20581 cggaatttac ttttgcccta ttacaattgg aaaaaccaat catagatatg atacggtaga
    20641 ttatatggag gtagacccta cattaggaga taaagagact ttaaaaaaac taatagaaga
    20701 agcgcataag agaaatataa aaataatgtt ggatgctgtt tttaatcata taggatatta
    20761 ttctaaacag tggcaagatg tagttcaaaa taaggaaaat tctcgatata aaaactggtt
    20821 ttatataaaa gatatgagta aaattgatac tccaatagaa cagatagatg aaaaaaatat
    20881 accttatgaa acctttgggt gtgaaaaata tatgcctaaa cttaatacag aaaattcaga
    20941 agtaataaaa tatttattgg atgtaggcaa gtactggata caggaatttg atatagatgc
    21001 gtggagatta gatgtttcaa atgaagtaga ccatgttttt tggagaaagt ttagagaaga
    21061 agtcaaaaaa gtaaaaccag acatttatat attgggagaa atttggcatg gtagtttacc
    21121 atggctaatg ggtgaccaat ttgattcagt tatgaattac ttgatgtcag aagccatgaa
    21181 aaaattcttt tgtacagatg agataaacgc cgaagaattt aagtatatga taaatgatgt
    21241 aatggtaagt tatccaatac aagttagtga agttattttt aatttacttg gaagtcacga
    21301 tactacaaga atattgactt atgctaatgg aaatattgat agatttaaac ttgcatatat
    21361 gtttatgttt gtacaaacag gatgtccttg tatatactat ggtgatgaaa tcggaatgga
    21421 aggagaatta agttcaatat ctgaaggtca aagaaaatgt atggaatgga atgaagaaaa
    21481 atggaataag gatatatttg attttatgaa gaagattatt agacttcgta aagaaaataa
    21541 agaacttagg tctatatcaa atgaatgggt gttagcagat aaggaaaatg gaacgattat
    21601 attgaaaaaa gaatctattt ctataataat aaataattca tctaaaaata taattttgac
    21661 gttaccagat tatttagcaa ataaaaaagt aaaagattta tatgaagaag aaatagttga
    21721 tttaaaagaa gaactaatgt tagagaaata caagttcatt attttaaaat aatacaaaaa
    21781 gtatttattt tagatttcat gttaaattat tatacagttt aaacaagtct ttataaaaca
    21841 taaaaaaata tggtttacaa gtgagtgaat tgaaagtaga ttttaaatta aagactgagt
    21901 tagagtagat aattttataa ataatatctt aaatacaaga tagaaaataa attgattatt
    21961 agtctaaaaa tttcttgtag aaagaggaaa taagaatgat tgaagcattt atatttgatt
    22021 tagatggtgt aattacagat actgcatatt atcactatat ggcttggaga aaactagccc
    22081 ataaagttgg gatagatata gacacaaaat ttaatgaatc tttaaaaggc ataagtagaa
    22141 tggagtcatt ggacaggata ttagaatttg gaaataagaa atattcattt tcagaagaag
    22201 aaaaagtgag aatggctgaa gaaaaaaata attactatgt atcattgata gatgagataa
    22261 cttcaaatga tattttgcca ggcatagaaa gcttgttaat tgatgtaaaa tctaataata
    22321 taaagatagg tctatcttct gcaagtaaga atgcaataaa tgtattaaat catttgggta
    22381 taagtgacaa atttgatttt atagctgatg caggaaaatg taaaaacaat aaaccacatc
    22441 cagagatatt tcttatgtca gcgaaagggc ttaatgtaaa tccacaaaat tgtataggga
    22501 tagaggatgc aagtgcaggt atagacgcta taaactcagc taatatgttt tctgttggtg
    22561 ttggaaatta tgaaaactta aaaaaagcaa atttagttgt agattcaaca aatcaattaa
    22621 agtttgaata tatacaagaa aagtataatg agtatatagt taggagaatt atatgaaatg
    22681 tttatttagt aatgtagatg agtggaagat tatacaagat aagattgaaa tcaaagaaaa
    22741 tagattagca gagtccataa tgagtatagg caatggatat atgggtatga gaggaaacta
    22801 tgaggaaaat tatagtggag atacacacaa ggggtcttat atagctggag tatggtttcc
    22861 agataaaact cgtgttggtt ggtggaaaaa tgggtatcca gaatactttg gtaaaattcc
    22921 taattcagtc aactatatag gtgtaagaat tttcatagat gatgtagaat tagatttagc
    22981 aaaatgtaaa gtagaagatt tttatagaga attagatatg aaaaatggta ttttaaatcg
    23041 taggtttatt gtaaatataa atgataaaaa atttgaagtt agggttacta ggtttttaag
    23101 tatatctgtt aaggaacttg cagtaattaa atatagtata aaatcattaa attctaatac
    23161 taaaataaaa tttgtaccat atttagattc aaatataaaa aatgaagatt caaactatga
    23221 ggaaaagttt tggaatggaa tttcttcaag ttctttagaa tataaaggta gtattctttc
    23281 tcgaacaaag gagaatgcat ttaatacacc agtattcact gtgggttcaa tgatgtatat
    23341 taagacaaat ggtgatgtag aagctgtatc tcatgagagt gattataatt actgtgaaaa
    23401 ttcgataaca atggatgttt taaaaaataa ggttgcaagt atagaaaaat atgtatctat
    23461 aacttcaact agagattata aggaaagtga gctattaaga aaatgtgaag acattttaaa
    23521 tatagaacta tctaaaagtt atgatgatat attaaatgaa catacaaagt tatggaataa
    23581 aagatgggaa agtgcagatg taaaaataaa tggagataaa agtagccaac aaggtataag
    23641 atataattta ttccaactat tttcaacata ttatggagaa gattcgcgat taaatatagg
    23701 tccaaaagga tttactggtg aaaagtatgg aggagctact tattgggata cagaagcatt
    23761 ttgtgttcca gtatatctag gtgtagcaga taaggaggta actagaaatc ttttagaata
    23821 taggtacaat cagttagaac aggcaaagga taatgcaaaa aaactagggc ttaaaggtgc
    23881 tttatatcca atggtaacat ttgatggagt agaatgtcat aatgaatggg agataacatt
    23941 tgaggaaatc caccgaaatg gaagtatagt atatgcaata tataactata caaattatac
    24001 aggtgactat aaatatataa aagaaaaagg aatagatgta ataatagaaa tagctagatt
    24061 ttgggcaagt agagttcatt tgtctacaaa aaaagatttg tatatgatac atggagtaac
    24121 aggtccaaac gagtatgaaa acaatatcaa taataattgg tatactaatt gtatagctaa
    24181 atggtgttta gattatgctg tagaaaatat cttacgatta gagaaagagt gtaatgaatg
    24241 tattgacaga aataatgtga ctagggaaga agttgagttt tggaaaataa tatcaaataa
    24301 aatgtatctg ccgtatgatg aggaactaca aataataatt caaaatgata attttttaga
    24361 taaagaattt gtagaagtaa aggatttacc aaaagaaaat tttcctctta atcaaaattg
    24421 gtcttgggat aagattctta ggtcgtgttt tataaaacaa gcagatgtat tacagggaat
    24481 ttactatttt ggaaatagat ttactaaaga agaaaaaaag agaaattttg acttctatga
    24541 aaaatatact gtccatgaat cttctttatc ttctagtatt tattctatta ttgcctcaga
    24601 aatagataac ctagaaaagg cttatgaatt atactcaaga acagctaggc ttgatttaga
    24661 taattacaat aatgatacaa atgatggact gcacataact tcaatgtctg gttcatggct
    24721 ttccattgta catggatttg caggcatgag aacatggaat gaaactctat cttttgaccc
    24781 taagttgcca aaagagtgga aaaattattc tttcaatata aattatagag ataatattat
    24841 taatgtagat gtatgtagtg atttaattaa aatagaaaat ttaaaaggta aaccaataaa
    24901 aattattgta tatgggaaaa aatatatatt agaaaaatat ataaaaattg ataagtagat
    24961 ttaataatat ttttattata taaatagtta aaataagttt atttaattat ttatctattt
    25021 taactatttt gtgtgataga aaactatata ttataacaat agtagtgcct ttaattaaat
    25081 atatttcata aatcttcata aaatcttcaa aatttcgtaa agaatatgtc acaaaagtaa
    25141 ggtatattat attcagcaga tagtataagg gaggacaata tttatgaaga ataaaaaatt
    25201 cattgttata atactgataa tatcaatttt aataggtgta ttagtgaagg aatattcatc
    25261 tagagagata aaaaaggatg atataaatgt cagtaagtat ataaaatatg ctgatttagc
    25321 aagtaaaaat aatgcacaag ttaactggaa gtatgtagct tccattgttg ctgtgttaaa
    25381 taaaaataac ttaaaaaacg ttaaggattc acagatacaa gaagtatcag atttatttgt
    25441 taagaatttt tctaaaaata ataaaataaa taagttaagt gatattttgg atgagttaga
    25501 atttagtaat agacaaaaaa gattggttga taattatata gacaatctta aggattatgg
    25561 tataaaacca gaaagattga agtctgatac aaaatatatg aagtttattg ctgaaattaa
    25621 aacagaagca atacaaaatt ataaggatta taaaatactt ccatctataa ctattgcaca
    25681 agctataata gaatctagct ggggcaagtc aactttggca aaacaatata acaatctatt
    25741 tggtataaag gctgacgctt attggaaagg taagtcagtg actttagaaa cgaaagaaca
    25801 tttagataca attatagatg ataaatttag aatatatgat gataaaaatg aatctataaa
    25861 agaccatgca aaatttttag ccacaaataa aaggtataaa aacaatggtg tatttgatgc
    25921 aaaaacgtat atatatcaag ctaaagcatt agaaaaggca ggttatagta ctgcgaagga
    25981 tgaaaatggc aatagtatat atgcagctcg tctaatagaa ttaattcaac agtataatct
    26041 tcaattaata gacagcgaaa ttcaaagtga agtgtaactt ttatgtaaca taggagtgat
    26101 atatttatcg aggaggtggt ttttatgaaa aaaatattct cgatatttat aagttgtatg
    26161 gttgttgtaa ctactattac tggatgtagt agtggtgagg tgataaaaaa tacatcacct
    26221 atcaatagtg ttaaaaattt ggattttagt tttgaggtga aacctgaaat atttgaggta
    26281 tcagttgaat ctggtggttc aaaagaaaag atatctgaac cctcaaaaga aaggaaagta
    26341 tcaaatttta aaaagaataa gacagaggca tcgtggacgt atcctgatga aaatatagac
    26401 ataaatataa aaaaagagga cagctattta gatgtaaata ttaaatctaa tttagaaaaa
    26461 gaaactagtt ttaattggcc caaagttgaa ggggaaagtt atatactacc actttatgag
    26521 ggtaagttta taccaagtaa tgataagtat tggaaggagt atctaaatga gtcaacttat
    26581 aatgttatag aatctttttc aatgcaattc tttgcagtaa acaaggcaaa gtattctgtg
    26641 atttatatta tcgaaaatga atttaacaat gtaattgaat ttagttctaa agacaagatt
    26701 aattttacat ttaaacatga atatccaagt atcaataaaa ataaggagta tgggtttaaa
    26761 atatatttga ctaataagaa tattacagat gtagcaaaaa cttataaaaa ttatatagtt
    26821 gataaaggag aatttaaaac attaaaagaa aaatctaagg aaaataaaaa tattgaaaaa
    26881 ctttatggag caccacatat ttattttgga gataagtcta tcatcacaat aaatgatatt
    26941 gaatggaata aattaaaaga aaatttatca gatgatttga ctaattggat gaaagagctt
    27001 ttaagtatga agtctggcaa tagtgaattt ataaaagtaa ttaatcaaat aaaaactcaa
    27061 gattatatag ataaatatca aaaaaatcaa atagtacaag catttaatga agttatgcag
    27121 atgaatactt tttataatga aaaagttttt gaaagcaaag atagagaaat aaaaaaagta
    27181 ttagataaag gtatatctaa attaagtagg gtagaactta tagagtttaa taagaaatta
    27241 ttgaaatcaa aattggaaga gagcgtgaat gatgttgcta catgggcaaa atataataca
    27301 actgatgtaa ttaatgatat taataagtct ggtattaaaa atgcgtggat aggatttgat
    27361 gatatggagg aaggttatat atccttagag atggttaaaa gagctaatga gttaggttat
    27421 ctcgtagggc catatgattc ataccactct atacacaaac caggggaaga aaagtggagt
    27481 acagcatcat ttaaagataa atctctatat gaaaatgcta ccatagaaaa taaaaaaggt
    27541 gaaaaagaaa ctggatttca aggagaagga agaaaactaa atcctacatt gtctttacca
    27601 agtgtaaaag agagagtaca aagtatattg gatgaaggat atgaatttaa ttcttggttt
    27661 atagattgtg atgctacagg agaaatctat gatgattatt ctcaaaatca tataacaaca
    27721 caaacacaag atttaaatgc tagacttgaa agaatgtcat atataagaga ccatgcaaat
    27781 atggtcatag gttcagaagg tggaaatgat ttttcaagta gtactatagc atttgcacat
    27841 ggtattgaga caccagcatt tgcttgggta gataaagata tgagtaagaa taaaggcagt
    27901 gaatattatg ttggtaaata ctattctcca acaggaggag taccagaaat attttcaaag
    27961 cagatacctg taaaagaaag gtacaaaaaa atatttatag acccaattta ctctctacct
    28021 ttatttagat tagtttataa tgattctgtg atatcttccc atcagtggtt atggggaact
    28081 ttgaagatta aggatgaagt tggaagtaga atgatgaaag aagtattata taatacacca
    28141 cctatgtatt atatagatag aactgaatgg gaaaaacata aagatgaaat aatctcaaac
    28201 aataaagtat ggtcagcgtt taataagaaa gctattaaaa aagaaatgac agactttaaa
    28261 tttatttctg aggatagact agttcaaatg actgaatatg gagaagattt aaaggtatta
    28321 gttaattttt ctcaaaaaga cattaagtac aatgatgaag tcataaaagc aaattctttg
    28381 gtaatatatg atgctgatag taaagtcata tataaaccgg aataatagat ttaatgaaaa
    28441 taaagattgt ttaaatttca agtaaaaaat aatataatat tcatttagtt tgtattaaag
    28501 tagtgacta
//
LOCUS       DAELXX010000044        28440 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.044, whole genome shotgun sequence.
ACCESSION   DAELXX010000044 DAELXX010000000
VERSION     DAELXX010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28440
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.044"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            121..1494
                     /locus_tag="KPH29_001828"
     CDS             121..1494
                     /locus_tag="KPH29_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG1467428.1"
                     /translation="MNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILANSF
                     FDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGKEITAINTTIPIDIKGEFVGAL
                     EISKNMTTIENLLNRNYKKNLVNKVSSRQKKGYEFDDILGESNLIKEAIEKSKKACKS
                     DASVFIYGETGTGKELFSQSIHYGSNRRNYPFIAQNCAALPESLFEGILFGTSKGGFT
                     GAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRII
                     ATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDILLLCKKFISKYNKKLNK
                     KVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYNLKEN
                     KESNNYSLNDLEGKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIK
                     KYNIKIL"
     gene            1750..2817
                     /locus_tag="KPH29_001829"
     CDS             1750..2817
                     /locus_tag="KPH29_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HBG1467429.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     DVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKIIFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMEEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGDVLIEMEHPQQIIPEVE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            2914..3222
                     /locus_tag="KPH29_001830"
     CDS             2914..3222
                     /locus_tag="KPH29_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-ketopentanoate thiolase"
                     /protein_id="HBG1467430.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKVNPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            3215..4645
                     /locus_tag="KPH29_001831"
     CDS             3215..4645
                     /locus_tag="KPH29_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HBG1467431.1"
                     /translation="MNNSTSIKDMSYQAVMGRNNEIMKNAIGLDYSSFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGAKIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKVIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNNL"
     gene            4723..5097
                     /locus_tag="KPH29_001832"
     CDS             4723..5097
                     /locus_tag="KPH29_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HBG1467432.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWNLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            5091..7295
                     /locus_tag="KPH29_001833"
     CDS             5091..7295
                     /locus_tag="KPH29_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBG1467433.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLRIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIVMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            7295..8704
                     /locus_tag="KPH29_001834"
     CDS             7295..8704
                     /locus_tag="KPH29_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HBG1467434.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIEYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELYIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            8689..9753
                     /locus_tag="KPH29_001835"
     CDS             8689..9753
                     /locus_tag="KPH29_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HBG1467435.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERSIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKKLQDINLPKILIRIPMLSELEEVINYCDISFNSELETI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFNNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            9783..11204
                     /gene="nhaC"
                     /locus_tag="KPH29_001836"
     CDS             9783..11204
                     /gene="nhaC"
                     /locus_tag="KPH29_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBG1467436.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDV"
     gene            11633..12097
                     /locus_tag="KPH29_001837"
     CDS             11633..12097
                     /locus_tag="KPH29_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG1467437.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLAKLDVEVGFPVATYIDGKNEILQRIVPAQKIITAIDL
                     GPYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            12395..12757
                     /locus_tag="KPH29_001838"
     CDS             12395..12757
                     /locus_tag="KPH29_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG1467438.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
     gene            13352..14212
                     /locus_tag="KPH29_001839"
     CDS             13352..14212
                     /locus_tag="KPH29_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1467439.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIIANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     CVEERDNRQN"
     gene            14470..16584
                     /locus_tag="KPH29_001840"
     CDS             14470..16584
                     /locus_tag="KPH29_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG1467440.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEHDVELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMAEKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHIVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REEYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYVGTLTQLISM"
     gene            complement(16846..19089)
                     /locus_tag="KPH29_001841"
     CDS             complement(16846..19089)
                     /locus_tag="KPH29_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG1467441.1"
                     /translation="MSNIIIKGLTQNNLKNISFKIPKNKIVVFTGVSGSGKSSIVFDT
                     IAAESQRQMNETYSSFIRNRLPKYRKPQVELIDNLTASVIVDQTPLGGNARSTVGTIS
                     DLYSSLRLLFSRIGEPYIGTTSYFSFNDPNGMCKNCSGIGRVKVINIESIINPENSWN
                     TGCINDSLFKPNSWYWKQYAESGLFDLDKPIKDYSFEEYNLLLYGSKNKDGKQENPKI
                     EGIFNKYNQTYLNRDISNMSKHTKEKSERLITDKECPVCHGKRLNKETLNCNILGYSI
                     ADMCNMELTDLYDLLLQIKDKRTETVIKTLTDGLKRMIDIGLPYLHLNRESSSLSGGE
                     AQRLKLVRYMGSSLTGMTYIFDEPSAGMHPRDVHRMNQLLKKLRDRGNTVLVVEHDKD
                     VISIADEVIDIGPSAGTQGGHVVFQGSYDKLLLANTLTSNAMNLSLPIKTDSRHPKGT
                     LPIRDACINNLKHIDVDLPVGIMTVVTGVAGSGKSTLISRVFAQKYEKDVIKIDQSPV
                     TATNRSMPATFLGFFDEIRKVFAKENNVEEGLFSFNSKGACPVCDGKGVLVTELVFMD
                     PIINTCEACKGTRYSEEVLSKLYKGKCIVDVLSMTVDESLEFFTEKKLCKILQTMKDV
                     GLPYMTLGQPLSTLSGGERQRIKLAKNLNKKGTIYILDEPTTGLHGSDIEKLMLIFEH
                     IVDKGNTVIIIEHNLDVLKQADWVIDIGPDGGKNGGQVVFEGTPLEMFERGCTITSDF
                     LRKSINI"
     gene            19421..20137
                     /locus_tag="KPH29_001842"
     CDS             19421..20137
                     /locus_tag="KPH29_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467442.1"
                     /translation="MILIVILSIFIIASINIIILGKENIRDIVIPLTLFIVSDFLFLC
                     GNIICRGGVMLLFIGLFAIPLMLIAFIWICVHMIKLYYKKDLNKRKMIGCIFSIFISI
                     GTIFIPSPSESFRFKLYEKDYSVVAKAILKVYDEKKLSSESVFYIQSDKNELEKIFSK
                     EVIKKMKKLNRNLDVVKISGNRNLVYFFFGAELQSIDGIVIFKDLKHVKPKLDFINRS
                     IQAPRLEYITNNVYYFKGEL"
     gene            20165..21103
                     /gene="blaCDD"
                     /locus_tag="KPH29_001843"
     CDS             20165..21103
                     /gene="blaCDD"
                     /locus_tag="KPH29_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBG1467443.1"
                     /translation="MKRKNNFIWIVILLVVVAMGVYYVGRKHNNTKQKHDKNHINSEL
                     NKSKVENNDKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETRRSPCS
                     TFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYYKKLID
                     KVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQTMAKI
                     FEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGMLEREGD
                     TYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            21447..22130
                     /locus_tag="KPH29_001844"
     CDS             21447..22130
                     /locus_tag="KPH29_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1467444.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            22127..24787
                     /locus_tag="KPH29_001845"
     CDS             22127..24787
                     /locus_tag="KPH29_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1467445.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAQKSKGESFKKISETTLTIVGKVDVSTSSVIPAYTALGYLDRTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKMS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYINSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKTVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNTRSRRQEFAMLRSVGLSPE
                     GIKKMLVLEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(24968..26149)
                     /locus_tag="KPH29_001846"
     CDS             complement(24968..26149)
                     /locus_tag="KPH29_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1467446.1"
                     /translation="MKTPRNINSTVVFIIFCLIITFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(26149..26811)
                     /locus_tag="KPH29_001847"
     CDS             complement(26149..26811)
                     /locus_tag="KPH29_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1467447.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCGNISLNKVEVKAYLDNVEVLLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DS"
     gene            complement(26956..27498)
                     /locus_tag="KPH29_001848"
                     /pseudo
     CDS             complement(26956..27498)
                     /locus_tag="KPH29_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(27616..28440)
                     /locus_tag="KPH29_001849"
                     /pseudo
     CDS             complement(27616..28440)
                     /locus_tag="KPH29_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT        10804 a         3447 c         4884 g         9305 t
ORIGIN      
        1 aaaaatatca taaatatatg ataagataaa tacaatagca atattttatt aattttttgg
       61 gtttttatat atttaggtac tggggtgata tagttgaacc aagagatatt ttttaaaata
      121 atgaatgaaa tattggaaaa tattgatgaa ggggtacatt ttgttaataa agacgggaaa
      181 actataatct acaataattc tgtacaaaaa atggaaaaaa tgaacagaca cgatatttta
      241 gccaattcat tttttgatat agtcaacaaa atgaaaataa acaggagtac actattagaa
      301 gttttggaaa aaagggaagt tatcaaagac aatattcaaa aatatctgga caaaaatgga
      361 aaagagataa cagcaattaa tactacaata ccaattgata ttaaaggcga gtttgtaggg
      421 gcacttgaaa tatcaaagaa tatgacaacg atagaaaatt tactaaacag aaattataaa
      481 aaaaatttag tcaataaagt tagtagtaga caaaaaaagg gttatgaatt tgatgatatt
      541 ttaggggaaa gtaatttgat taaagaagca atagaaaaat ctaagaaagc ttgcaagtct
      601 gatgcttcag tatttatata cggtgaaact ggaacgggta aagagttatt tagccagtcc
      661 attcattatg gaagtaatag gagaaactat ccatttatag cacaaaattg tgcggctttg
      721 ccggaatcac tattcgaagg tatccttttt ggaactagca agggaggatt tacaggagca
      781 atagataggc caggcttatt tgaacaagct aacaaaggga cattgttatt ggatgagata
      841 aattccatgc ctattatgtt acaggctaaa ctattaaggg ttttacaaga aggttatgta
      901 agaagggttg gaggaactaa ggatatacct atagatgtaa gaattattgc aacgacaaat
      961 gaaagtccaa aggtaatttt aaacgagcat aaaatgcgaa aagatttata ttataggctt
     1021 aacataattc atataaaaat tcctcctctt agagatagaa aagaggacat acttctattg
     1081 tgcaaaaaat ttatttcaaa gtataataaa aagttaaata aaaaagtggt aggaatttca
     1141 gatgaagcta ttaaaatttt aaaaaattac gattggcaag gtaacataag agagctagaa
     1201 aacataattt attgtactat gagtatgatg gaagatgagg tacaaataga aacaagtatg
     1261 attaatctaa acgactatta caatcttaaa gagaataaag agagtaacaa ttattcttta
     1321 aacgatttgg agggcaaaac acttagtaag attatcagtg agatagaaga aaagtatata
     1381 tatgaatcat tagaaaaggc atcgtacaat ataactaaag ctgcatcaat tttaggtatg
     1441 aataggcaaa atttacagta taaaataaaa aaatataata tcaaaatatt ataaataagc
     1501 aagataaaaa aacaaaatag aagtaaaata aaaaagcaaa atataggagc aaaattatta
     1561 taaaagagca aaatattttg ctcttttttt tatttctcat aagaaatatt attttttatt
     1621 tatgaaaata cataataaaa taattatagt ttcaacgttc caataaatat ttactttaat
     1681 tttaaagttg gcacgattta tgctttaact aattattgag aatatataaa tttttaggag
     1741 ggtgtatata tgagaaaagt aagagtagga atatggggat ttggagctat gggaataggt
     1801 atggctaata tgattctaaa gaaagaaggt atagaaatag tatctgtttg tagtagaagt
     1861 acttcaggga aaagtatgta tgatgtttta ggaattgaaa gaggagaaag accagaagtt
     1921 attatcaata aaaattatga agaagttttt agagaaaaaa gtgtagatgt tgttttgtta
     1981 gcaactgatt catttactaa aaaggctttt gataaaataa ttttcttatt aaatagaaaa
     2041 ataaatgtta tatcaacagc agaacaaatg gcatatccac aagctgatga tgcagattta
     2101 gctaaaaaaa tggatgaagt tgcaaaggaa aatggagtaa gtatacttgg aacaggaata
     2161 aatccaggat ttgtattaga tttactagta ttggcacttt caggtacttg tgaagaagtt
     2221 actagtataa aagctaagag agtaaatgat ttatctccat ttggaaaatc tgttatggag
     2281 gaacaaggtg ttggagtaac aagggaagaa tttataaaag gtgtagaaga taagactata
     2341 gcaggtcatg taggttttgt agaatcaata aatatgatag ctgatggatt aggttggaaa
     2401 ttagataaga tagaacaaac aaaagagcca ataatgacta cagtagatag aaaatctaaa
     2461 tacggagagg cattggctgg aaatgttgct ggttgtagac aatgtggata tggatatgta
     2521 aatggagacg ttttaattga aatggagcat ccacaacaga taataccaga agtagaaggt
     2581 attaaaacag gagactatgt atcaataaaa ggtataccaa acattgattt acaaataaat
     2641 cctgaaatac ctggaggagt aggtacttat gcaatgatag taaactctat tccattaata
     2701 ataaatgcta gaccaggtct taaaactatg ttggatatac cagtgcctag agcaataatg
     2761 ggagacataa gaaatcagat agaagttgaa ttggaagaag aatcaaaggc taattagtaa
     2821 gataacagtt agctaattgg taaaataatg tttaatttaa aataacagta aattaattag
     2881 taaggaatag tcaattaata aggggatgaa aaaatggatg ctaaaattaa tgattgggtt
     2941 attatacaca atatagtatt gactccagaa gaaagagctc cacaagttcc agaggatact
     3001 aagaaagtat ccttagaaat gtgggttaaa ggttttatac aaagtgatgc ttctataggc
     3061 gacttagtag aagttaaaac aattacaggt agacttgtaa aaggtaattt acttaaagta
     3121 aatccatatt atactcatga ttatggtaaa tgtataccag agttactcca aataggaatc
     3181 caagctaaag aaatattatt tggaggtgta tataatgaat aattcaacat caataaaaga
     3241 tatgagttat caagctgtta tgggaagaaa taacgaaata atgaaaaatg caatcggatt
     3301 ggattattct tcttttgaac aagagggaat agggtttgat tatgagaaaa tgatgagtga
     3361 aacaggatat acattacaag atattgaagc aattcaatca cagtatgctg ttggaaatac
     3421 tcctctaata gagcttaaaa atttgactaa attagctaga aaatgtgcta aagaaggtaa
     3481 aggagctaaa atatttgtaa aagatgaagc aatgaatgca tctggtagtt ttaaagcaag
     3541 aagagcagct actgcagtat atcatgccaa acaaatggga tataaaggtg taatagcagc
     3601 taccagtggt aactatggtg cagctgtagc atcacaagcg gcaatgcagg gattaaaatg
     3661 tataatagtt caagaatgtt atgattctaa tggtgtaggt caacctgaaa ttatagaaaa
     3721 agcacgaaaa tgtgaggcat atggagcaga agttgttcag ttaactgttg gaccagagtt
     3781 attttatacg ttcttagttt tattagaaga aacaggctat tttaacgcat cattatattc
     3841 accatttgga attgcaggtg tagaaacttt aggttatgag ttagctatcc aatttagaga
     3901 aaagtataaa aaagaccctg atatagttgt ttgtacaaat gcaggtggtg gtaatcttac
     3961 tggaactgca agaggattga taaaagctgg ttccattaat actaaagtag taggagcaag
     4021 tgtagattta aaaggattac acatggctag tgataatcaa tttaataaaa aatcatttac
     4081 aacaggacat acaggttttg ggataccata ctgtacatgg cctgataggt cagatgtacc
     4141 taggtctgca gctagaccac ttagatatat ggatagatat gtattagtaa aacaaggaga
     4201 agttttttat acaactgaat tattggcaca gctagaaggt atagaaagag gtccagcagg
     4261 aaatacatct ttagcagcag cattctcttt agctcaagag ttagatgagg ataaagttat
     4321 agtggtacaa gaaacagaat atacaggcgc aggtaaacat atatgtccac agcttacttt
     4381 tgcaagagaa aatggaatag atataaaatt tggaaatcca agagaagaaa ttgcaggagt
     4441 aaacttaata ttaccagaaa gaccagaatt attgaaatgt gttgatattg atatgaataa
     4501 aataagaaag tcatttataa aaaattgcat atctaataat cacattgata atattgataa
     4561 gctaagtaat cgagatatag aatttttaat ggaagaagta aaaagttcaa gagattttgt
     4621 tgttgatgta ttaaataatt tatagtaatt attgtttttt tatttaatat aaaataaaaa
     4681 ttcggaaaat tctattaaat caatgcgaat ggaggaatgt tcatgaaaaa aagagaggat
     4741 gattttgaag ttagaagaaa acatcttcaa gaactgagtg atgatgagtt aaaagaaagg
     4801 ttttggaatt tagctaccca gatagtagaa ccaatgatag aacttggtaa aaagaataca
     4861 acaccatcaa tagaaaggtc tgtcttgttg agaatgggat tttcttctct tgaagtaaaa
     4921 cctattttag aaggggttat ggaaagaggt cttataggta aaggagcagg gcatgtagtt
     4981 tataaattgg ctaaatctaa aaatataacg gttagagaag caggtttgtt attagttaag
     5041 ggtgaatatt gggatgaagt tacatgttta tttagagaag gggtagaatc atgttaaagg
     5101 aaaataaaaa attggatata gactatatat taaaagattt ggataaatat aaaccaaaac
     5161 gaagaggttg ggtttggaga gaacacttgg aaaatcttga aatgggacct tttaaatata
     5221 aagactgtac aaaaccttta aagaagagtg tagggctacc atcatctaag tatttcaata
     5281 atatagaccc tcaaccaagt ccagttatta ctactgaaat agcttctggt agatttgagg
     5341 atgatataag aagaatgaga atggcagctc atcatggagc tgaccatcta atggttatca
     5401 gaactgctgg acaatcacat tttgatgggc ttatagaagg aactccacaa ggtataggtg
     5461 gagttccaat aacaagaaaa caagtaagag ctcaaaggaa ggctcttgat tttatagaag
     5521 aagaagttgg tagacctatt aattaccatt catatgtaag tggtgttgct ggtcctgaag
     5581 tagcagtaat gtttgctgag gaaggagtca atggagcaca tcaagaccca caatataatg
     5641 ttctgtatag gaatataaac atggtaaggt cttttgtaga tgcttgtgag gcaaagaaaa
     5701 taatggcttt tgccaatata gctcaaattg atggagcaca taatgctaat gctactgcta
     5761 gagaagcatg gaaggtaatg cctgaactta tggttcaaca cgctttaaac tctatattct
     5821 cagaaaaaat aggtatagat aaaagtaata tatgcttatc aacagttcca ccaacagcac
     5881 ctccagcacc atgtttaaga atagacttac cttacgctgt tgcacttaga gaattattta
     5941 gtgattataa gatgagagct caaatgaata ctaaatatat ggaatcatca actagagaag
     6001 caacagttac tcatgtatta aacttattaa catcagtatt aactagagct gacatacaat
     6061 caactataac acctgatgaa ggtagaaatg taccttggca tatatacaat atagaagcat
     6121 gtgatacagc aaagcaagct ttagttggta tggatgggct tatggatatg atagaactta
     6181 aagatgtcgg tgaattaaga gacaaagcaa gagaacttaa agaaagagct gtattatata
     6241 tggaagaaat atctgaagtt ggtggatatt ttgaatctgt ggaacaagga ttttttgttg
     6301 attctggaaa ttatccagaa agaaatggtg atggaatatc tagaaaaata aaaggtggag
     6361 ttggtgaagg tactgtttat gaaagagaag aagattattt agcacctgta actgctcact
     6421 ttggatacaa taatgtagct caatatgatg aaaatgctat agataatcca tcaatgttaa
     6481 tagatggatg tacatttgaa aatccagaca aaataatata tattgatgag cttgacgatt
     6541 ttgataatgt agaaaataga ctaaaagaat catatgagta cagaaatgga actaaaatta
     6601 agcctgaaat ggaatggtgt gcagatggaa ttgtaatgat aacaatgatg cttcctactg
     6661 ataaaagaac agcagagttt gcagctttgg aatttgtcaa aaaaatgaac ttacaagaaa
     6721 ttgaggtcat aagtagagaa gttatgcatg aatctgaagg gactagaata gaagtgaaag
     6781 gaagagttcc gtttgatata gatttaaaca atttagtaat accagaagaa cctaaggtac
     6841 taactgatga agaaataaga gcagatatag aagaaaaacc tatgaaaata gtttcagcta
     6901 cggttggaga agatgaacat tcagttggtt taagagaaat aatagatata aaacatggag
     6961 gtattgaaaa atatggcata gaatgccact atctagggac ttctgtacct gtagaaaaat
     7021 tggtagatgc agcgattgaa ctgaatgcag atgcaatact tgcatctaca ataataagtc
     7081 atgatgatat tcactataaa aacatgaaaa aattacacga ttattgtgta gaaaaaggta
     7141 taagagataa agtaatgatt gcgtgtggag gaacacaagt aacacctgaa atagctgttg
     7201 aacaaggcat cgatgctgga tttggaagaa acagcaaagg catacatgta gccactttct
     7261 tagttgaaaa aagaagagaa atgagtaata aataatggaa aatagcaaag tttctttaaa
     7321 aatagatgtg ttggtagctg aaattggaag tactactaca gtggtaaacg ctttccacga
     7381 tattaatact aacaatccta tatttttggg tcaaggtcaa gctccaacaa cagtgtttga
     7441 aggtggcgat gtaagaaatg gcttatcagg tgctattaaa gatttagcta ataaattatc
     7501 tgtagatgat atagagtata atgatatgtt tgcaacttct agtgcagctg gtggattaaa
     7561 aatgactgtt catggattag tctatgatat gacagtaaaa gcagctaaag aagcagcttt
     7621 aggggctgga gctgttattc gtcaaataac atcaggtaga attaaaagaa ctgatttaaa
     7681 taaaataaaa gaaataaacc caaacataat tttaatagct ggtggagtcg attatggaga
     7741 aagagataca gctatttata atgcagaaat gatagcttct atgaatctag gcataccagt
     7801 tatatatgct ggtaatgttg aaaatcaaga ggaaataagg ttaatatttg aagatactaa
     7861 ttataagctt tatataactg aaaatgtata ccctaaaata gatttattga atatagaacc
     7921 tacaagaaga atcattcaaa gtgtatttga agaacacata actacagcac caggaatgaa
     7981 atacattaaa gaaatggtta atgaaaatat tactccaacc ccaggagctg taatggaagc
     8041 ttctaaactt ttatataaaa atattggaga tttattaact ttggatgtag gtggagcaac
     8101 tactgatgta cattctgtta cagatggaag tgactatatt aataaaattt tggttaatcc
     8161 tgaacctact gcaaagagaa gtgttgaggg ggatttaggt gtatatgtaa atatgaagaa
     8221 tatagtagaa gttataggta aagaaaactt acaaagtgaa ctatatatag atattgatgc
     8281 agtaatagag aattatcctc caataccaaa atcaaaagaa gaaatattat ttgttgagag
     8341 gttgacaaaa gaagctgttg ttaaagctat gctaagacat tcaggaaaaa taagaaatat
     8401 ctatggaaca actggaaaag tcaaaattgc agaagggaaa gatttaacag aagtaagata
     8461 tatagttggt acagggggag cacttactag gctccctagt agaattgaaa tattagataa
     8521 tatgctcaaa tacaataaaa ataatgaatt actatttcca aaggaaaaaa caaagatatt
     8581 aatagataat gactatatta tggcatctat gggtgtttta tctaaaaaat atgaagaagc
     8641 atctcttaag ctcctgttaa aaagtttgaa ttttgaggag gaaagattat gtatcctagg
     8701 ttagaaatag atatagaaaa gttaaagcat aatgcaaaat taatttcgca gatgtgtcat
     8761 gaaagaagta ttaagatatc ttttgttact aaatcatttt gtgcacaaaa agaaatagta
     8821 gaagaaatat gtagtgaagg aatagaccat atagcagatt caagaataca aaacttaaaa
     8881 aaattacaag atataaatct acctaagata ttgattagaa tcccaatgtt aagtgaatta
     8941 gaagaagtta tcaattattg cgatattagt tttaactcag aattagaaac aataaagaaa
     9001 ttaaatgaat tatgtgaaag taaaaatatt attcataaga tagttcttat gtttgactta
     9061 ggcgatttac gagaaggata cttttatgaa gaagactttt ttaataatgt aagagagata
     9121 gtaagattaa aaaatattga aataattggt atagctacaa acttaacctg ttatggagct
     9181 attataccat ctagggaaaa tataggcaga ctggtaagta tagcaaaaag aatggaagaa
     9241 aaatttggaa taaatctaga aatagtatct ggaggtaatt caagttctat tcatctgttg
     9301 ataaataata atatgccaga aggtattact aatttgagaa taggcgagtc cattctactt
     9361 ggtagagaaa cggcatacgg tgaaaatata aatggaacat atcaagatgc atttaaatta
     9421 atatgccaag tagttgaatg taaagaaaaa ccatcagttc ctattggaga aataggagtt
     9481 gatgccttta gaaataaacc tgtttatgaa gataagggaa tcttaaaaag ggcaatcata
     9541 gctataggta aacaagatat aaatattgac tctttaattc caatagacac tgatataaaa
     9601 atattagggg caagttcaga ccacatgata ctagatgtaa gtaacacaaa atatgattat
     9661 aaattaggtg acaatttaga atttttatta acttatgggg gtatcatgtc atctagtaca
     9721 agcaaatatg tttcaaaaaa aattatagtg tagtgtttaa ataattagga ggaaactatt
     9781 ttatgaaaga gcgagaatgt agaaaagcaa gtctgtttga tgcaatgata ccaattactt
     9841 gtttgatatt atttttaagt ctaggagtat tagtgtatgg ttcatcacct catgtaccac
     9901 taataggagc agcagcagta gcaggacttg tggctgtata tagacttgga tttaaatgga
     9961 aagagttaga attaagtatg tttaatagta taaagatggc aatgcaagca atcttaatca
    10021 taattataat aggcgtactt ataggaactt ggatagtaag tggagtagtt ccatgtatga
    10081 tttactgggg actaaaaatt ttatcaccaa atattttttt agtagcatca acattagtct
    10141 gtgcaatagt atctcttgcg acaggttcat cttggagcac aatgggtaca gtaggagtag
    10201 ctcttttagg aattggtcaa agtttaggta tgccaattgg attaatcgta ggttctataa
    10261 tatctggagc ttactttgga gataaactat cacctttatc tgaaacgact aacttagctc
    10321 cagcaatggc aggaacagat ttgtttacac atataaaata tatgttatat tcgactatac
    10381 catcattatt aatttgttta gtaatttatg gtgtaattgg aatgaagtat tcaggtcaag
    10441 cactagatat aaaacaaata gaattaatta ggtctacatt agattctaca tttaatactt
    10501 tatcaccagt attattatta gctccagcaa tagtaattgg attagttgta tttaaagttc
    10561 ctgctatacc agggcttata gtaggtgctg tattgggagt attgtttgca gtagtttttc
    10621 aaggagaatc tatgaatacg atattagatg cagcacaaaa tggatatgtt tcgtcaacac
    10681 aaatacagga ggtagatgct ttattatcta aaggtgggat tatgaatatg atgtccactg
    10741 tatccttaac aatatgcgct ttatctcttg gaggtatttt agaaaaaaca ggtatgcttg
    10801 aagtagtagc atcttcttta ctaagattag caaagggtgt atttggaaca gtattatgta
    10861 ctatggtaac ttgtacaatt accaatataa tagcaggtga acaatatcta tctatagtta
    10921 taccaggaag aatgtataat aaggaatata aaaagagagg tatacatcct aagatgttgt
    10981 cgagagcttt agaagatagc ggaactttaa cttctccatt agtaccatgg aatacatgcg
    11041 gggcgtatat aacagcaaca ttaggtgtat ctgcactaac atatggtcca tacgctttat
    11101 taaatattat aaatccaata gtatctttag tattaattgc atgtaagttt aaaatagcaa
    11161 gaatagaaga tgaacctgaa acttgtttaa attttgatgt ttgataatag ataacttatg
    11221 taagaacgta ggaactaggg ggggagcctg tggggaatta gtaagacata ttttagtgtg
    11281 cgaaattcta taaatcagta atcttgtgac tttagccatg ggaggttcag attagtaaat
    11341 ttatagtttt atattagtct atactttgat cttttaattg tttgtataaa ttttttatag
    11401 aaaaagattt cttttagact tgcctaaaaa ttaggcaagt cttttaagta tgttctgatt
    11461 ataagagcac tgtgtgacaa gaatgatgag attatttttg ctatactttc gatataagaa
    11521 atggaaggag gttatgtttt atatttatat gaaataatta gttacacagt gaattttcgt
    11581 ataatttggt ataaaaagat tagaaatgaa aataaagtaa tggaggaata aaatgactag
    11641 aatatccaat ataacaatag ctgaacaatc tgagtactgt tttttggcaa tacgaaaaac
    11701 aattgacttt atggtagagt tttcagagtt ttcaaaacaa agttttgaaa aaatttctaa
    11761 atatctggag gagagaggaa ttttatcttc aggagcaccg attgtatgtt ttcataacat
    11821 ggatttagca aaattggatg ttgaagtagg gtttcctgta gccacttata tagatgggaa
    11881 aaatgagatt ctacaaagga ttgttcctgc acagaaaata ataactgcaa ttgatttagg
    11941 accttatgaa ttacaagacc ctactttaga agacttattt tcatgggcaa atagcaatgg
    12001 atataaactt catggcgata tatattacca gtatttaaat gatatcaatc gtccagctag
    12061 tgaatatctt acgaaaatga tgttacccat tatatagaaa gttaaagatt atcgtttgtt
    12121 agttgataat tagaagaaca tttaatgcta ctgcataaat tttatcctaa ataatatcaa
    12181 ggtgatttaa tttatgtatt ctaatgtaac tgtagataat caatttataa ctaacttagt
    12241 gtaatttaga tttaatttat tattctaatg taaccccaat atattgttaa ggtctactta
    12301 agatgctcaa aaggctatgc ttaatttatt gttatataat ttgtagagtg gtataattta
    12361 gaagtgatac tatatagata tataggggga aaatatgaaa atatatgata tattaacacg
    12421 tttttatatt caagatatag aaaaagccat acctttttat gagaatcttt taaaagaaaa
    12481 atgttctttg cgtttttctt ataaagaagt tgggttggaa ttagcgcaaa taggaaatgt
    12541 tttactatta tctggttctg atgatgctct taaaccattt atagaaacaa aatctacttt
    12601 tatggttgat tctgttgatg aatggagaag ttatctattg gataatggag cagttgtagt
    12661 aagagataag aaaaaagttc ctacaggata taatatgaca ttgagacatc cagatggtac
    12721 aattatagaa tatgttcaac atacaaagca ggattagtta aaaatagatt ttactactga
    12781 gcaactaatt agaatctatt cttagacgta caatttatta tttttggtat taatagatta
    12841 taagtcaaat tataatattt caaaatagaa tagaaattta tttacataaa agagagtgtt
    12901 taaaaatgaa ctttttagtt cacgaagcac tcctttttgt atgaaatcaa atacatcaat
    12961 atataaaaca aactgatgaa gagcttaaat taaaataaaa agattcataa caggacccca
    13021 ttaaacttta agatagggta agtttgtaat catattaaat aattgtaaaa agctgttttt
    13081 agtcgtataa tataaatatt tatcatttaa ttaaggaatc taaaaggaat ctaataagaa
    13141 gaaaataata aataactttt tagatttact aaatgaaaaa ttattgaaaa ttaaaggatt
    13201 agagtcttta atcagaaagt aaaaagtaat atattaaatt tatgaattga aaacttaaac
    13261 aacaaatttg tttataaaat agcaagattc attaaatata actaagtaat aattgatata
    13321 atgttgcatg gataaaagga gggaagctgc aatgtcttac aaagtaatta atgtcataac
    13381 tgctattgac tatatagagg agcatttatc agaaaagctg gatttagata taattgctaa
    13441 tgctgttcat tactcaaaat atcacctgca tagaactttc acaacttctg ttggtctaac
    13501 aatgcatgat tacataaagc ggagaaagtt gacagaagca gcaaaattat tggtattttc
    13561 aaagaaacca attattgaaa ttgcattgat agctggatat gaaagtcaac aggcattcac
    13621 ttctatattt aaagctatgt ataaaaaatc tccaaataaa tataggaaag aacaagaatt
    13681 ttatcctttg cagctaagat ttgttttgaa aaaagataac ctattttcaa agaatgtact
    13741 agagttggaa aaagaaatta aacttgcaag tatgtctgac ataccattat ggatgaattt
    13801 agtgagatta gtcatagatg gatttccaaa tttgcaagag gaagaatata tatatcaatt
    13861 agaacagtat attttagaag aaagagcttt aatattaaaa cttaataatg tcgctattgc
    13921 aaatatggtg tttaatagag aaactagaag tattgatttt tttggaatac atccacagta
    13981 tcgtaattca gacattgcac aagtgttttt gaagaaagtt attgaagatt tcttgataga
    14041 tacagatatc tcaattacta cctttcgaga gggtgacaaa gctgatacag gacatagaga
    14101 tatgataaag aagttggggt ttgcagaagc agagttgctt gtggaatttg gatatcctac
    14161 tcaaaaattt attcttcctt gtgtagaaga aagagataat agacaaaatt aattgtagat
    14221 taaatgcaaa gtttaccaca tattttttgt ttgaattatt ttatatataa gagtatttct
    14281 gcttttaatg tattttgctt gtaagtgtac ttatggtaat aaatatagaa agatttagtt
    14341 ttttaaaaaa agaactttaa agctaaaaag ctaacattta agttaaatag caagaatgat
    14401 taaaaactcc aaaacacaat taagtataat gattcagtgt aagtacttaa ttaacaaagg
    14461 aggatttaaa tgtctgaaaa taaacaagta gtaaattgcg atattattgc atccaattct
    14521 aaacataata tgtgtgaaca tgatgtagag ttattaactt tttttccaga tgagattgca
    14581 catctaatag agataaataa caagctagac aatgctttta agaaagctga aaatcttgtt
    14641 gacaagcttg ataaggatta tatggacgct aaaatgtata tggtaaaaaa tcgtggtgaa
    14701 atagacccac atgaaatgtt tcaaaatgaa caaggactaa aacaaataga taactatggg
    14761 gcttttatgg taaaagttcg agataagatt aataaaatca aagattctcc atactttgct
    14821 agaatagatt ttaggctgaa agatatggat gatgaatcta agtattatat tggtagattt
    14881 gcatttgatt atgaagatga gcttataata cttgactggc gttctccaat tgccagtatg
    14941 ttttatgatt atgaaatcgg aaaagctgga tatgatgctc caataggatg ggtggatgga
    15001 gaaataacaa gaaaacgaca atttaaaatt aaaaatggaa aattggaata tgcactagaa
    15061 agttccatta atatacaaga tgatattctt caaaaagaac ttagtcacac ttcagatgaa
    15121 aaaatgaagt caatcatttc aacaatacaa aaggaacaaa atcaaattat tagaaatgat
    15181 aaagctgata cactgattat tcaaggggta gctggttctg ggaaaacatc tatagcatta
    15241 cacagaattg cttttcttct ttatcgattt aaagataaaa tatcagcaaa taatgtaatt
    15301 atcctatcac ctaataaagt ctttggagac tatatttcca atgtccttcc tgagcttgga
    15361 gaagaaccat tatgtgaatt aagttttgaa aatatagcag aggtacaatt agatagagtt
    15421 attaattttg aaagtgagaa agacccatta gaaataaatg atgctaaatg gtctgaaaga
    15481 gtgcgtttta aatctacact tgattttgta aaacttattg atgattatat aaaacaaatg
    15541 ccaaataaga tttttattcc aaaagactac acttttggaa gttttacagc taaaagtgac
    15601 tggatacaga gtagatttga ggcatataac agatatccag ttaagaagag attagaaaag
    15661 gtagctgaag acattcatta taaatttgaa tcagataata ttatggaaga agatttacca
    15721 aaggtaaaaa gtatacttaa aagtttgaat ggaatgttga caataaaaaa tactcttaca
    15781 ctatataagg atttctttaa acaaatgaat atatcaaaca tgtttgtgat ggcagaaaag
    15841 aaaacacttg aatggtcaga tgtataccca tttatatata ttcatgctgc atatgaaggt
    15901 atacaagaag ataagattat aaggcatatt gttattgatg aaatgcagga ctatacacct
    15961 attcaatatg ctgtaataaa cttacttttt aagtgtaaaa agactattct tggagatttt
    16021 ggacaattgg ttaatcctaa tcatactcat acattagatg atatgaaaca gctatataat
    16081 gatggggaac tagttatgtt gaacaagagt taccgttcta cttttgagat tattaatttc
    16141 gccaaaaaga ttcaagatat atcttcatta gagcctattg aacgacatgg tgaagaaccg
    16201 gctttattaa aatgtaataa cgagcaagat gaaataaata agataaaaat agaaattgaa
    16261 gaatttaaaa agagtgataa tgcgactctt ggtattatat taaagacaga taatgacgct
    16321 aaagctgtat atgatgcgtt aagagaagaa tatagtgttc acttaatatc ttctgaaagt
    16381 tctagcttta caaaaggagt atccataact tctattaaaa tgtcaaaagg tttagagttt
    16441 gatgaagtta ttgtaccttc agtaaataat aaaacatatt atagtgatta tgaccgcagt
    16501 ttgttatata ttgcttgtac acgagctatg cacaaactta agttaacata tgttggtaca
    16561 ttaactcaac ttataagcat gtagattata tcttatatgc atttattatc gtaaatataa
    16621 attgggacaa agattcatca ataaatcaac aaaaaataat acttataaat ttataaaaaa
    16681 tgaaagctat aaattacatt tgaaatttta taaaattttg tgatttatag cttttataat
    16741 attaatttct tttaaaataa agttaaactt taaatttatg aaataaaaca aagtagtaat
    16801 atatatgtaa tagttcttaa ttttttaata ttagtattat attatctata tatttattga
    16861 ttttctaagg aaatctgatg ttatagtgca acctctttca aacatttcta gtggtgtacc
    16921 ttcaaatacg acctgaccac cgtttttgcc accatcagga ccaatatcaa ttacccagtc
    16981 tgcttgtttc aatacatcca aattatgttc aattataatt acagtattac ctttatcaac
    17041 tatatgctca aaaatcaaca taagcttttc aatatcagaa ccatgaagcc ctgtagttgg
    17101 ctcatctaaa atatatatag tacctttttt gttgaggttt ttagctaact taattcgttg
    17161 acgttctcct ccagatagtg tgctaagtgg ttgacctaag gtcatataag gtaaaccaac
    17221 atctttcatt gtttgtaaga ttttacatag cttcttttct gtaaaaaatt caagagattc
    17281 atctactgtc atagatagta catcaactat acatttacct ttatataatt ttgacaaaac
    17341 ttcttcacta tatctagtac ctttgcaagc ttcacatgta ttaataatag ggtccatgaa
    17401 tacaagctct gtaacaagta ctcctttacc atcacaaaca ggacaagctc cctttgaatt
    17461 aaagctgaat aaaccttctt caacattatt ttctttagca aatacttttc ttatctcatc
    17521 aaagaaacca agaaatgtag caggcataga acgatttgta gctgtaactg ggctttggtc
    17581 gattttaata acatcttttt cgtatttttg tgcaaaaaca cgagagatta aagtactctt
    17641 gccagaacct gctactccag taacgacagt cataattcca actggtaaat ctacatcaat
    17701 atgttttaga ttatttatgc aagcatctct aattggaagg gtaccttttg gatgacgaga
    17761 gtcagttttt ataggtaatg atagattcat tgcatttgaa gtaagagtgt ttgctaaaag
    17821 taatttatca taagagcctt ggaatacaac atgtccaccc tgagtaccag cacttgggcc
    17881 aatatctata acttcatctg ctatggagat aacatcttta tcatgctcta ctacaaggac
    17941 agtatttcct ctatctctaa gtttttttaa tagctgattc atacgatgta catctcgtgg
    18001 atgcatacca gcactaggtt catcgaaaat ataagtcata cctgtgagac tgctccccat
    18061 atagcgtaca agttttaatc tttgagcttc tcctccagat agagatgatg attctctatt
    18121 tagatgtagg taagggagac caatatcaat cattcttttt aaaccatcag taagagtctt
    18181 tattacagtt tcagttcttt tatcttttat ttgtaacaat aaatcataaa ggtcagtaag
    18241 ttccatattg cacatatctg caatcgaata tcctaaaata ttacagttaa gagtttcttt
    18301 atttaatctt tttccatgac atacaggaca ttccttatct gttataagtc tttctgattt
    18361 ttcttttgtg tgtttgctca tattgctaat atctcgattt agatatgttt gattgtattt
    18421 gttaaaaata ccctctattt ttggattttc ttgttttcca tctttatttt ttgaaccata
    18481 aagcaataaa ttatattctt caaaagaata atcttttata ggtttatcta aatcaaaaag
    18541 tccagactct gcatactgct tccagtacca tgaattaggt ttaaataaag agtcatttat
    18601 acatcctgta ttccatgaat tttcaggatt tataatagat tcaatattga ttactttaac
    18661 tctaccaatc ccagaacaat ttttacacat accatttggg tcattaaaag aaaaatatga
    18721 agttgttcct atgtagggtt ctccaattct agaaaataat aatcttaatg atgaatacaa
    18781 atcacttata gtgccaactg tagaacgtgc attgccacca agaggagttt ggtctacgat
    18841 tacagaagca gttaaattat caatcagctc cacctgaggt tttctatatt taggtagcct
    18901 atttctgata aatgaagaat atgtttcatt catctgcctt tgtgattctg ctgcaattgt
    18961 atcaaatacg atactggatt ttccagaacc agaaactcca gtaaaaacaa caattttatt
    19021 tttaggaatt ttaaatgata tattttttag gttattttgt gtaagtcctt ttattataat
    19081 atttgacatg aatgttaaat cctttctgag tacattttag taaagcaatt atattatgta
    19141 accatattat aataaaatta aaatctactt tcttgatata ttctgctatg ttatagaagg
    19201 tatttctaaa tttaaccaat cccatacaat ctgaataaat gatagttgat aatagataaa
    19261 aaagtatata atagatactc gtaatttgat aagtacaaag ataattatac aattaaaacg
    19321 acttgacatt taaaacttac tattgcaata tttaaatatt acaatagtaa gttttaaata
    19381 gagaataaat aattacctca caaactgaaa ggaggacata atgatactaa tagttatatt
    19441 gagtatattc atcatagcct caatcaacat aattatatta ggtaaagaaa atataagaga
    19501 tatagttatc cctttaactc tatttattgt aagtgatttt ttatttttgt gtggaaatat
    19561 aatatgtagg ggaggagtaa tgctactatt tataggttta tttgcaatac cattgatgct
    19621 aattgcattt atatggattt gtgtacatat gattaagctt tactataaaa aagatttaaa
    19681 taaaagaaaa atgattggat gtatatttag tatctttata agtataggta ctatatttat
    19741 tcctagtcca agtgaaagtt ttagatttaa actttatgaa aaagattata gtgtagtagc
    19801 taaagctatt ttgaaagttt atgatgaaaa aaagttaagc tctgaatctg tcttttatat
    19861 tcaatctgat aaaaatgaat tggagaagat attttctaaa gaagtaataa aaaaaatgaa
    19921 aaaactaaac agaaatttag atgtagttaa aatttctggg aatagaaatt tagtctattt
    19981 tttctttgga gctgaattac aatctattga tggtattgtg atttttaaag atttaaaaca
    20041 tgttaaacca aagttagatt ttataaatag aagtattcaa gctcctagat tggagtatat
    20101 tacaaataat gtatattatt ttaaaggtga actataaact tatgtagagg agatggtata
    20161 aagtatgaaa agaaaaaata attttatttg gatagttatt cttctagttg ttgtagctat
    20221 gggagtatat tatgtaggaa gaaagcataa caacacaaaa caaaaacatg ataagaatca
    20281 tataaactct gaattaaata aatccaaagt tgaaaacaat gataaaaaca aaaaagaaaa
    20341 aattaatatg gttgactata gtgactgttt tgaaggtatt agtggaggag ctatttttta
    20401 taatactaaa aataaagaat ataatatata taacaaagaa ttaattgaaa caagaaggtc
    20461 accatgttct acttttaaaa tagtttcaac tttaattggt ttagaaaaag gggtaataaa
    20521 ctcaaaagaa tcagttatgg gatatgatgg tacagagtat ccaaataaaa attggaataa
    20581 aaatcttagt ttagaagagg catttaagga gtcttgtgtt tggtactaca aaaaattgat
    20641 agataaagtt gatgctaaat ctgtccaaaa cattttagat gatttaaagt atgggaattg
    20701 cgatattagc gaatgggaag gagatttaaa aaatggaaag ggacatttaa atggattttg
    20761 gttagaatct tcattacaaa tatctccaaa agaacaagtg caaacaatgg caaaaatatt
    20821 tgagggagat acaaacttta aaaaagagca tattaatatt ttaagagata ttatgaaaat
    20881 agatgtaaat gataaaaaca taaatgtata tggaaaaact ggaacaggat ttgatgaaaa
    20941 aaataaacgt gtggatgcat ggtttgttgg tatgctagag cgtgaaggtg acacctatta
    21001 ttttgctatt aaatcagatg attccaataa agaaattaca gggccaaagg taaaggaaat
    21061 tgcaattaat ataattaaga aatattatag tgtaagggaa taaacttaat tttatttgta
    21121 aatattattt acctatagtc tgtaatattt attaaataat tttactaaat tgagtctata
    21181 tagagcaaaa ctcctttagt acataaaata cttgctattg attattaaat tgaataccta
    21241 tagatttaaa gttaatacct tttaaagact atgtaatata ttaaaagtat aaataaaata
    21301 tattgcatag tttttgtttt atattataat tttaaataat gtgcaaaaac taaacaatat
    21361 ttttaatagt aatttatatt tgtattcatg tcactataat gtcattttag gttgctaaaa
    21421 tttaaccata aaggagatga ttgtatatgg aaatattaaa atgtgaaaat ctcacaaaaa
    21481 tatatggttc aaatcaaacc agagtgacag cccttaataa tgtaaattta tcagttcaaa
    21541 aaggtgattt tgtatccatt gtaggagcat ctggttctgg caaatctaca ttgttacatc
    21601 tgcttggggg agtagataga cctacatctg gaaagattta tgttgaagat acagaaatat
    21661 caagtttaaa ggaagaagct cttgcagttt ttagacgtag aaaagtaggt cttatttatc
    21721 aattttataa tcttatacct acccttgatg ttagaaaaaa tatattatta cccatgttac
    21781 ttgataaaag aaaggtggat gaggatagat tctctgagat tgtatctata ttgggattat
    21841 ctgatagatt aaaccatctt ccaagtcaac tttcaggtgg acaacaacaa agggtatcta
    21901 tagctagaag tttaatctat agaccagcta ttttattagc agatgagcca actggtaatc
    21961 ttgatagaaa aaattcagaa gaaattgtag acttgttaaa cttatcaaat aaacgattta
    22021 atcagacaat acttcttatt actcatgatg agaaaatagc actagaggca aatcgtattg
    22081 taactatgga agatggagtg attgtctctg aaaaggtggt gaagaaatga atattcttca
    22141 agaatacacc ttagactttg tacgacgcaa taaacgtagt agtatagcta taatgttagc
    22201 tatattactt actacaacca tgatgtcttc tttgtgtgga ctgttgtata ccatgtggac
    22261 agattttata agactttcaa ttgagaaaga aggtaactgg catggtgaat tgtttgacta
    22321 tacttatgga agtgacttac cattaattga aaatttttct tcagttgatg atgtaatggt
    22381 aaaaggacaa tggtatgtag gcaaaattaa tgatgagaaa agagattata taatttatag
    22441 aaatgctaca tcagagtatt ggaattctat gccagaaaag gatactataa tagagggtcg
    22501 tcgaccaaat aatgctaatg aaattgcatt atcaaaacaa tattttgaga ataatccaag
    22561 tgtaaaaatt ggggataaaa ttactcttcc aataggagat agaatcctaa atggaaagcc
    22621 tcttgaaccc atagctcaaa aatcgaaagg ggaaagtttt aaaaaaattt cagagactac
    22681 attgacaata gtaggaaagg tagatgtttc cacttcatca gtaatacctg catatacagc
    22741 tcttggatat cttgatagaa caagtataaa acctaatgat agtataactg tgtacttgcg
    22801 atttaaaaat ataagagaca catacaagga acttccaaaa cttgctaaat cattggggtg
    22861 gaagactaat gaatatggaa aatacaatct taaatataat agtaattatc ttttaaaaat
    22921 gttagtcttt tccccagaac agaaagcatc tatgagcagt atatcaaaat tttcaactcc
    22981 tattatgtat cttacaatag caatttttac agtagctgtg tttgtaatgg tgatttacaa
    23041 cgcgttttca ctatctgcaa atgctagact tactcaatta gggatattat ctagtgttgg
    23101 tgcatcacca aaacagatta aaatgtctgt agtgtttgaa ggttttttgc ttacaattat
    23161 accattgcca ataggtttat ttttaggatg gttattgtgt aataggctta tagtttatat
    23221 taattcagtt aattatcata ctgatgttcc agaagtggta tttacttatg gtattcctgc
    23281 ttttctgcca gcagttttac ttacaatagt aacagtctgg atttctgcac ttataccagc
    23341 acgaaaagta tcaaagatat ctcctataga agcaatacga caaggagata gtgttaaaat
    23401 caaaaagtcg cataaatatt cattaggaaa gatatttgga atagaaggag aacttgcatc
    23461 aaacgcacta cgtgctcgta aaaaatctta tcgtactgca acaatatctt taacattatc
    23521 ttttttatta cttacatgtt ttttgcatat aattgcaaat caagaaggag caaaagctgt
    23581 ttatggaagt gataattata aggaccaaag agatatagca gtttatttgg aaaatagtga
    23641 acctgtagag tctgctttca ttgaaaaatt agataataca gaaggtgtaa aaagttcact
    23701 atattatcaa aaaatgtctg cttccacttg gctgactgaa aaagatgcat caaaagaact
    23761 tgcgaaaaat ggaggattta aaagtatagt ttcatctaaa aaatactctc ctatagaacg
    23821 tgatggcaag tttcgtataa tttcaaatat aattgggctt gatgataaga gttttacaga
    23881 atattgtagg caaatttctg tagacccaaa gttattttat gatacaaaaa atccaacctc
    23941 aattgtatat aataaggagg aagatattaa tcgtagtaca cgtagaaata aagtatatat
    24001 agattattta aatctttcaa taggagataa cattaaatta aatgaaaaag tatatgacga
    24061 agataaaggg gattatactt acaacatgaa agtgggagct attacaagca cacttcctaa
    24121 aacagtagat actagttcaa aatatacatt aatgcaaata gtgccaatgt ctatggtgca
    24181 ggaaatatct aaaaattatg atgaagtaag taggttacgt tcaaataggc tgataggagt
    24241 atttaaagta aataatcgtg aggacattcc tagagtaaga gaaaaattag agtccattta
    24301 taataaagac tatggttcag gagattatag tattgaggat gttttagaaa gtgaaaaaca
    24361 aaaggctagt ggaataaaga ccatgaattt aatagtaggc tttattacag gtttattggc
    24421 tatgattgga ttatcaaatg tacttgctac agtgtcagga aatactagaa gtagacgaca
    24481 agagtttgct atgttgcgtt ctgttggatt atctccagaa ggcataaaaa agatgcttgt
    24541 tttggaaggt ttgttcttag gaattacacc attactactt agtattcctg tgcaaatagg
    24601 aatagtgtat gcttttctac gtataaatga aatatatttt atagagtatt taccatttgc
    24661 acctatatct acaataatag gttttactat attgatactt tttattgtaa tagcatcata
    24721 tatgacagga tacaagcagt tgaagaatga aaatatagta gaatctataa aaaatgaaac
    24781 aatataaaca aaagctattt gaacttattt aaaggtttcg atttaagact acttacaagt
    24841 atagggatag ttaatttgtt tgtttaataa aaccttaaat gtaaatagaa gtatattact
    24901 aaaacaatag ataacataga atatagatat agctggatta taggtatttt acctataatc
    24961 cagcatttta tgtgtaaaat ttaattatga atcttgcacc accattatgg caattttctg
    25021 catgtagagt accattttga cgttctataa tagactttga tagagcaagc cctataccaa
    25081 tactgtcctt actagcattt ttaccacgat aaaatctatt aaaaatatat ggaagttctt
    25141 cctgagcaaa ccctttacca ttatcttcta tgataatctc aacatataga ggattttgac
    25201 tataataagc aactatatat ccaccatctt ctatatgctc actacaattt ttaagaatat
    25261 ttaaaagagc ttctaaagtc caattaatat cgatttcaaa ttctaccgaa ggagaagcct
    25321 cgatagtaaa tgtctgatgt ttttctctta aagaaccttc tataggctca attgcataag
    25381 taagaagaga atgtacatct ataagtttgc tttcaaatat taaagtactt gcatccaatt
    25441 ttgataatgt aagtaaagat gaaactagtc tctcaagtct tgagatttga tttgtaagtc
    25501 tgttaatata atctatttct tcatctggac aattttctga aagtaattgt gccataagtg
    25561 acatagaagt aattggagtc ttaagttgat gagcaatatc tgcaagatta tctgaaagag
    25621 tttgtctttc ttttaaagca aattcttttg aacaacgaag ttccccaact gttttatata
    25681 tctcatcttc aaggtgagaa aattcatctt cacatcgtaa aagtacagac tcttttccta
    25741 aattcacttc ttctaagtat tgagtaagat tgtttattct actcagtttt tgcttatttg
    25801 ttctatgttt gattatatat acaactgtag ccattgttga aattatacca aacgatatca
    25861 ctagaaaata aaaaaagttt ttactccaaa atgtactttt agaatatcca tgttttgata
    25921 aaatttcatt tcctgtttta aaatccttat cactagaatt tttcagtgat tttagtatac
    25981 tttctgtaga cttaggattg tactcatatg atgcaccaag agccttagac agtacattgt
    26041 attcattaaa agaagttata gttgttagaa ttacacttaa taaaaaagtt ataattaagc
    26101 agaatattat aaaaacaact gtactgttga tatttcttgg agttttcact agctgtcctc
    26161 cattctatat cctatgcctc ttaatgtttt tatacaaggt ggatttttta atttttctct
    26221 aagacgcttt attgtaacag taagagtgtt atcatttacg aaattaccat ctacatccca
    26281 gagcatttct agtaactttg tacgggtcat agctctattt ttattttcca taagacttag
    26341 aagaagttta tattctcctg ctgtcaaaag tacttcaaca ttatctaaat atgctttgac
    26401 ttcaacttta ttcaagctta tatttccaca tgtaatagta ttgttagtat ttgaatgagt
    26461 tcttctaaga actgcattaa ttctggattg aagtactgag agcagaaatg gtttaaccac
    26521 atagtcatct gcacccatat ctaatccacg tataatgtct ttttcatcat cacggactgt
    26581 caaaaatatc actggagtat cttctatact tttaacatat tggcaaaact cgtatccaat
    26641 accatcaggt agatttaaat caagcagaat taaatcaata gttttatcaa aatttttttt
    26701 agcttcagca atcgtattat aacaacatac agtgtgattt ttcttttcaa gggaagtttt
    26761 tattccaaat gtaattgtat catcatcttc taaaagaagt attttagcca tttactttta
    26821 gtcctttcag taatttagtt atttcattaa ctaagttatt agttttatgt ttaatatttt
    26881 ttatgttaga gatagaagtt agagatagaa aaaattatca tttaagtata taaactttaa
    26941 tttataaact taggtttatg ctttaattga atctattaaa agagcaagag ttgtttcaat
    27001 aaatttttcc tcatctatat taaagttgtt caggtatgac tttcctgtta cagatagtaa
    27061 attaaaaaat cctgtaagtg taaatattga tgaatacatg agagtaggta tttcaatatc
    27121 atgcctaata cttctatcat tttttccaat ttcaaacatt ccttgtattt catcaaacat
    27181 aaatttatta aaagaaaaaa acttttcttt aaacagcata tcagtatccg atttttcaga
    27241 gtttacaata ccaatcattc ccataaggca taatagagaa ggttctacag agtaaaattt
    27301 atgaaaagca aagtatgaaa gttttatttt ttcaaaccct gttttgcctt ttgcactttc
    27361 tattttaaca ttatcccaaa gtcttttata tcctcttaag acaacagcaa agaataaatc
    27421 ttctttacat gaaaagtact tgtatatggt gcgtttagta tattcaactt cttttgccaa
    27481 ctcattcata gttgtattct gaacctccca tgactaaagt cacagggttc ctgcttcata
    27541 gaattttgca tactaaaata tgtcttacta attctccaca ggccatcccc gtagttccta
    27601 cggttcttac ataagttatt tattttgaat cgttattagt cttaatcctt cttttaatat
    27661 atttatactt gcatttatat ctctatcgtg agtttcatta caactaggac aaatccattc
    27721 tcttatattt aagttcttaa cttcctcgtt tttataccca catttattgc atatttgtga
    27781 acttgcaaag aatttaccta ctttatacaa tttgtctacc ataccaatta gccttgtatt
    27841 ctaattgacg aataaattct gaccatgata catcagaaat taagcgagat agtttatgat
    27901 ttttaatcat attctttact tgtaaatctt ctatgcaaat aatatcattc tcttttatta
    27961 atttagtaga taatttttgt agaaaatcat ttctttgatt agctatatgt tcttgaagtc
    28021 ttgcaacttt taatcttgct ttatttctat ttaaactacc gattgtcttt ctcgataatt
    28081 ctctttgtaa tttagcaagt ttgtttaatg acttcttaag atacttagga ttttctataa
    28141 attctccaca acttgagata caaaattctt tgattcctaa atctaaacct gtctgattgt
    28201 tagtattttc aaatacttca atatctacat cagtacagca taatgataca taatatctac
    28261 cacttggttc tttagatata gtagcattaa gtattctacc ttgaggtact tgtttatccc
    28321 ttatcttaac cataccaagt ttaggtagtt ttatatgttg accacaatac ataatgttcc
    28381 catttgtaaa gttagtttta tatgaaaatc tattaatttt ctttgattta aatttaggaa
//
LOCUS       DAELXX010000045        28253 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.045, whole genome shotgun sequence.
ACCESSION   DAELXX010000045 DAELXX010000000
VERSION     DAELXX010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28253
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.045"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            70..1674
                     /gene="rho"
                     /locus_tag="KPH29_001850"
     CDS             70..1674
                     /gene="rho"
                     /locus_tag="KPH29_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBG1467448.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQENRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            1827..2027
                     /gene="rpmE"
                     /locus_tag="KPH29_001851"
     CDS             1827..2027
                     /gene="rpmE"
                     /locus_tag="KPH29_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBG1467449.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            2172..3065
                     /locus_tag="KPH29_001852"
     CDS             2172..3065
                     /locus_tag="KPH29_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HBG1467450.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            3083..3931
                     /gene="prmC"
                     /locus_tag="KPH29_001853"
     CDS             3083..3931
                     /gene="prmC"
                     /locus_tag="KPH29_001853"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBG1467451.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            3975..5039
                     /gene="prfA"
                     /locus_tag="KPH29_001854"
     CDS             3975..5039
                     /gene="prfA"
                     /locus_tag="KPH29_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBG1467452.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            5168..5848
                     /locus_tag="KPH29_001855"
     CDS             5168..5848
                     /locus_tag="KPH29_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBG1467453.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            5877..6917
                     /locus_tag="KPH29_001856"
     CDS             5877..6917
                     /locus_tag="KPH29_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBG1467454.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            6934..7383
                     /locus_tag="KPH29_001857"
     CDS             6934..7383
                     /locus_tag="KPH29_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HBG1467455.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            7400..7852
                     /gene="rpiB"
                     /locus_tag="KPH29_001858"
     CDS             7400..7852
                     /gene="rpiB"
                     /locus_tag="KPH29_001858"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBG1467456.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            7888..8517
                     /gene="upp"
                     /locus_tag="KPH29_001859"
     CDS             7888..8517
                     /gene="upp"
                     /locus_tag="KPH29_001859"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBG1467457.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            8817..9632
                     /locus_tag="KPH29_001860"
     CDS             8817..9632
                     /locus_tag="KPH29_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HBG1467458.1"
                     /translation="MKNCIAVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            9858..10295
                     /locus_tag="KPH29_001861"
     CDS             9858..10295
                     /locus_tag="KPH29_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HBG1467459.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            10480..10707
                     /locus_tag="KPH29_001862"
     CDS             10480..10707
                     /locus_tag="KPH29_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HBG1467460.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            10712..11098
                     /locus_tag="KPH29_001863"
     CDS             10712..11098
                     /locus_tag="KPH29_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HBG1467461.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAIRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            11101..11805
                     /gene="atpB"
                     /locus_tag="KPH29_001864"
     CDS             11101..11805
                     /gene="atpB"
                     /locus_tag="KPH29_001864"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBG1467462.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            11874..12134
                     /gene="atpE"
                     /locus_tag="KPH29_001865"
     CDS             11874..12134
                     /gene="atpE"
                     /locus_tag="KPH29_001865"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBG1467463.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            12257..12754
                     /gene="atpF"
                     /locus_tag="KPH29_001866"
     CDS             12257..12754
                     /gene="atpF"
                     /locus_tag="KPH29_001866"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBG1467464.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLAEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            12751..13296
                     /locus_tag="KPH29_001867"
     CDS             12751..13296
                     /locus_tag="KPH29_001867"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBG1467465.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            13313..14815
                     /locus_tag="KPH29_001868"
     CDS             13313..14815
                     /locus_tag="KPH29_001868"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBG1467466.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            14843..15721
                     /gene="atpG"
                     /locus_tag="KPH29_001869"
     CDS             14843..15721
                     /gene="atpG"
                     /locus_tag="KPH29_001869"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HBG1467467.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            15734..17128
                     /gene="atpD"
                     /locus_tag="KPH29_001870"
     CDS             15734..17128
                     /gene="atpD"
                     /locus_tag="KPH29_001870"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBG1467468.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            17131..17391
                     /gene="atpC"
                     /locus_tag="KPH29_001871"
     CDS             17131..17391
                     /gene="atpC"
                     /locus_tag="KPH29_001871"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HBG1467469.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
     gene            17853..18233
                     /locus_tag="KPH29_001872"
     CDS             17853..18233
                     /locus_tag="KPH29_001872"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBG1467470.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRLFNFKDIEVLRNEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
     gene            18248..18709
                     /locus_tag="KPH29_001873"
     CDS             18248..18709
                     /locus_tag="KPH29_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBG1467471.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            18730..19320
                     /locus_tag="KPH29_001874"
     CDS             18730..19320
                     /locus_tag="KPH29_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467472.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQGDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            19335..20492
                     /gene="alr"
                     /locus_tag="KPH29_001875"
     CDS             19335..20492
                     /gene="alr"
                     /locus_tag="KPH29_001875"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBG1467473.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEESVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            20525..20812
                     /locus_tag="KPH29_001876"
     CDS             20525..20812
                     /locus_tag="KPH29_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HBG1467474.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            20781..21164
                     /locus_tag="KPH29_001877"
     CDS             20781..21164
                     /locus_tag="KPH29_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBG1467475.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            21335..22162
                     /locus_tag="KPH29_001878"
     CDS             21335..22162
                     /locus_tag="KPH29_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBG1467476.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            22155..23096
                     /locus_tag="KPH29_001879"
     CDS             22155..23096
                     /locus_tag="KPH29_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBG1467477.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGINAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIIKTVKEAIIAKRM"
     gene            23519..24613
                     /locus_tag="KPH29_001880"
     CDS             23519..24613
                     /locus_tag="KPH29_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467478.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDKLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPVIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDKHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
     gene            24839..25255
                     /locus_tag="KPH29_001881"
     CDS             24839..25255
                     /locus_tag="KPH29_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467479.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(25437..26006)
                     /locus_tag="KPH29_001882"
     CDS             complement(25437..26006)
                     /locus_tag="KPH29_001882"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBG1467480.1"
                     /translation="MKKEFRKKVIESRKNKSANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGAYGIR
                     EPNEKSPTIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNN"
     gene            26160..27317
                     /locus_tag="KPH29_001883"
     CDS             26160..27317
                     /locus_tag="KPH29_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HBG1467481.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGAAKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
     gene            27504..>28253
                     /locus_tag="KPH29_001884"
     CDS             27504..>28253
                     /locus_tag="KPH29_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBG1467482.1"
                     /translation="MFNKNEILKKLIEEYDVKTTTDIQDMLKDLFASTIHQMLEAELD
                     DHLGYDRYDNKNKNTKNSRNRYRNKNVKSDFGEITLNIPRDRNGDFEPKVIRNYENDI
                     SGIENQVIGMYSKGMSTRDISSHLKSIYGIDISHSLIAKITDRVLPLAHEWQSRALDM
                     VYPIVFMGAIHYKVRSEGRIINKAAYIAIGVNLEGITEVLGIWIGENESSKYWLKVLT
                     DVKNRGVKDILITSVDGLVGFSDAIKAVFPDT"
BASE COUNT        11140 a         3092 c         5447 g         8574 t
ORIGIN      
        1 tttaaataaa acttttcttt ttcatgcctt caatttccaa aataagtatg tatataaatg
       61 aatatataga tgaggaggat ttttttgatt ttagatgata aagtattaga aggattaggt
      121 agtaagacct tagttgagct acgagagata gctaaagaat taaaaataaa atcaattact
      181 acatataaga aaaatgagtt aattgagatt ataaattcca aaagtaaaaa tgaggaaaaa
      241 gtaaacgaag agactaaaga aaaaaatgaa gatattaaag aagacataaa gaatagaaaa
      301 gaaaatatag agcgtgaaag tatagaaaaa gaagttgaat ataagagccc taccaaatca
      361 tacaacaaga aagaaataga tacccaaaat atttcccaaa accaagaaaa tagaaatcaa
      421 agaaatgact ataataagac aacgagtgta aatgatagta ataagtcatc aaatatatct
      481 aatagaatgg tcagaaataa taataaaaac tattatatgc ctaaacaagt tgatgaatct
      541 aagatagtag atgaatttaa cacatcaaaa gaggatgaag tagtaggtgt acttgaaatt
      601 ctgccagatg gatttggttt tttaagaggt tcaaattatt tgtctactga aggagatgtt
      661 tatgtttcac catctcaaat aagaagattt aatatgaaaa caggagacaa gattaaggga
      721 ataactagac atccaaaaag tggagagaaa tttagagcac ttttatatgt tcaaaaaata
      781 aatgatgaaa accctgacac agctatacaa agaaatgcgt ttgagacatt aacaccaata
      841 tttccagaag aacgacttac actggaaact aatagaaatg aaatagctac aagaattata
      901 gatttaattt cacctatagg aaagggacaa agaggattga tagtagcacc tccaaaggct
      961 gggaaaacag ttcttttaaa aagcgttgca aatagcatag ctaaaaatca tccaaatgtt
     1021 gaacttatag tactccttat agatgaaaga ccagaggaag ttactgatat gaaagaatct
     1081 attgaaggag acgttatata ttcaactttt gaccaagtat caagtcatca tgtcaaggtt
     1141 gcagagatgg tgttaaatag agctcaaaga ttagttgaac atggtaaaga tgtagtaatt
     1201 ttactagata gtataactag attggcaagg gcatataacc ttacaatatc tcctacaggt
     1261 agaactttat ctggaggtat tgaccctggt gcacttcatg gacctaaaaa gttctttggt
     1321 gcagcaagga atattagaca gggtggttct ttgacaatat taggaacagc attggttgaa
     1381 actggttcta ggatggatga tgtaatattt gaggaattta aaggtacagg aaatatggag
     1441 ctacatttag atagaaaatt agcagaaaaa agaatattcc ctgcaataga tatttacaag
     1501 tctggtacaa gaagagatga tttattgctt gatgatgaag aaaagactgc tctttggaga
     1561 ttaagaagag aaatgagtaa taattcagta atggaaatta cagataaagt cattgaactt
     1621 ataaagagaa ctaaggacaa taaagagttt gtaaaatcaa taaagaattt gtaaaatcaa
     1681 taatacaaat gcaaatatgg attatataaa ctaatagaaa aatcacttga atggcataat
     1741 aagttgtgct ataataatgt agttgataat gtaaaaagaa agttaatata attaattttt
     1801 ttaaataaag agaagaggtg actataatgc aaaaggaaat acaaccaaaa tacaatccag
     1861 ttgaagtgcg ttgtgcttgt gggaacacat ttgttgctgg ttcaactaag gacgaaataa
     1921 aagttgaaat atgttcagag tgccatccat tctatacagg aaaacaaaag aacatagaaa
     1981 aaggtggaag aatcgacaaa ttcaagaaaa gattcaaaat ggactaattt ttataaaagt
     2041 ggagttggag gaagccaact cctattttat tacaaaaaaa cattcatatt ctactttaaa
     2101 ttgtcttttt gtattataat aataaatatg taaaattgag tattctaaat acaataaaat
     2161 gaggtgagtg gatggcaaaa caagctgtag gtgggcaagc tgttattgaa ggtgtaatga
     2221 tgcagtcaaa agacaaaaga gcggtagctg ttagaaaaag tgacggagaa atagcactaa
     2281 aagaagatag aataaaaagt tgggtaagag ataagaatat agataagata ccttttgtta
     2341 gaggttcttt tgttatgata gatacaatga ttcaagggat aaaaagttta aacttttctt
     2401 cagagttttt tatggaagaa gctgaagagg ataaatttga tttatttata aagaaaattt
     2461 ttaaagataa ggcaaatgat ataattataa tattttcatt ggtcatagca atgttacttt
     2521 ctgctggatt attcattttt atacctacat tggtaggagg agctttttct aaagtaatgc
     2581 ctaatgattt tatgcttaat ttaatagaag gaatcataag gatagctata ttatttgcat
     2641 atatagtatt gatttcacga agtaaggata tagaaagagt ctttcaatat catggagcag
     2701 aacataaatc tatatattgc tatgaaaatg atttagaact tacagtagag aatgctagaa
     2761 aatttaaaag attacatcct agatgtggta caaatttcct atttatagtt atggctgtat
     2821 caataatatt gtttgcattt tttggttggc caaatccaat tcttagaata tttatgagaa
     2881 ttatatgtgt gcctatagta gcaggtttat cttatgaggt aattagagtt cttgggaaat
     2941 atgataatgg atttactaaa attattgcat atccaggaat gatgttgcaa tattttacaa
     3001 caaaagagcc tgatgatgag caattagagg ttgctttgga agcattgaaa gcagtagtag
     3061 attagagaaa aggatatttg ttatgacgat aaaagatata attattaaat attcagataa
     3121 actaaaagat ataagtgata ctccaagatt agatacagag ttattgttac aaaaaacatt
     3181 gggtgttgat agattatata ttcacttaaa tctgaataaa gagctaactg aagaacaaaa
     3241 aacaaaattt atgggttttg cagaagaaag attaaatgga agaccaatag catatatagt
     3301 tgaaaatagg gagtttatgg gattagattt ttttgttaag gaaggcgtat tgattccaag
     3361 accagataca gaaactttag tagaagaaat aattgaaata tgtagagaaa aaaaagatgt
     3421 aagtatatta gatataggaa ctggttctgg agctattaca ataagtttag caaagtacat
     3481 tgaaaattct aaaatcatgt cttttgatat atcagagaca gcattagaaa tagctaaaaa
     3541 aaatgctatt ataaatgaag ttggtgaaaa aataaagtat attaattctg atttatttac
     3601 agcaataagt gatagtaaca taaaattcga tatcatagtt tcaaatccac catatattaa
     3661 aaaacaagat atagaaactc tgcacaaaca agttaaagat tatgaaccat ataatgctct
     3721 tgaaggtgga gaagatggat tagactttta tagaagaata acagaacaag gtaagaaata
     3781 tttaaataag tgtggaatat tagcatatga agttggtcat aatcaagctg aagatgtaat
     3841 aaatattatg aaaagtaatg gatataaaaa aatatacaca aagaaggata ttcaaggcat
     3901 tgatagagtt gttataggtt acaatatatg atataatcaa caattgatat gttacattta
     3961 atcgaggtga aagtatgtta aaaaaattag aggtattgga agatacatat aaagacttaa
     4021 gtgaaaaaat aggtgatccg gatgtaataa atgatcaaaa agtttggcag aaatatataa
     4081 aagaacatgc tgacttggaa cctattgtta tgaaatacag agagtataaa agcgttttag
     4141 atagtataaa agaatcaaaa gaaattttac aagaagagtc agatgaagaa ttaagagaat
     4201 tagctaaaat ggagttagct gagatggaag aaaaagtagc acctctggag gaagaaataa
     4261 aaatactatt attgcctaaa gaccctaatg atgataagaa cgttatagtt gaaattagag
     4321 gtggagctgg aggagatgaa gcagctctat ttgcgggtga tttatttaga atgtactcaa
     4381 ggtatgctga aagaagaaga tggaaaatag aattattgag tgcaagtgat actggagttg
     4441 gtggatataa agaagtatct tttatgataa aaggtaaagg tgcttattca agattaaagt
     4501 atgaatctgg agttcataga gttcaaagaa taccatcaac tgaatctggt ggaagaatac
     4561 acacatcaac ttctactgtt gcagtattac cagaagttga agatgtagag gttgaaataa
     4621 atccaaatga tttaagaata gatgttttcc gttcttcggg taatggagga caaagtgtca
     4681 atactactga ctctgctgtt agggtaacac atatacctac aggtgaagta gtatcttgtc
     4741 aagatggaaa atcacaatta aaaaataaag agcaagcttt aaaaatatta aaagctagac
     4801 tttacgataa agcattggca gaacaacaca aagatatagc agcagaaaga aaaagccaag
     4861 tcggaacagg agacaggtca gaaagaataa gaacatataa tttccctcaa ggtagaataa
     4921 gtgaccatag aataaattta actttatata agttagatgc atttttagat ggagatatag
     4981 atgaaatgat agatgcttta attactgttg accaaacaga aaaaatgact gcaatataag
     5041 taatgtttta aagaagctgt ttcttcctag aaattatttt ctattttgaa acagctattt
     5101 tgcattttag gtcatgttat catattctat tgtatataat tgttatatac aagtatagga
     5161 gggttatatg atactaaaag taacaataat tgggcttttg gcaggtgtta tagggacagg
     5221 attaggagga gtaatatcag ccatttttaa aagagaggtc gataaatatc taagtttttt
     5281 tatgggatta tctggtggaa taatgttggc tgtagtggtg tttgatttaa tgaaagaatc
     5341 catggacaaa atggggataa tcaatacagt tatatttacg tttgttggag ctctcataac
     5401 tatgtacata aaaacaaaat tagatgtgtc tggaaatatg gcatctggat atcttatatt
     5461 cataagtata ttactacata atttaccaga aggactagcc attggttctt cttttatgtc
     5521 aactgaaagt ttaggtatta cacttgctat agttataggt ctacataata taccagaagg
     5581 gttagctatg gcattaggct tagtttgcaa taaaatgaaa ctaagtaaag ttattttgtt
     5641 tactgtaata gctggattac ctatgggtct aggaagtttt ttaggtgttt attttggagg
     5701 agtatttact tctcttatag gagtatttct tgctacagca ggaggtacta tgatgtatgt
     5761 tgtattggaa gaaatttttc cacattcaaa aagtgtgtat tgtataattg gtttcttgtt
     5821 aggtacaatg attgtaaatt atatataaaa ttaaaatgaa agcaggcata caaaagatga
     5881 aaactattat aagtaatatt gatataaata atattgatta tgaggaaata aaaatacaag
     5941 caaaacttct tagagaaggt aaaacagtta tttttccaac tgagacagta tatgggttgg
     6001 gtgcaaatgc attagatgaa aatgcagtaa agaagattta tgaagctaaa ggaagaccta
     6061 gtgacaatcc attgatagtc catatatatg aaaaagaaga agtatatgac ttagccaaag
     6121 atataagtga taaagctaaa cttgttatag aaaagctttg gccaggacct ataacaatta
     6181 tattaaataa aaaagatata attccataca aaactagtgg agggcttgat acagttgcaa
     6241 taaggatgcc ttctaatgtt attgccaggg caataataaa agaagctggt atacctattg
     6301 cagctccttc tgcaaatatc tcaggtcgcc catctccaac aaaagcaaaa catgtatatg
     6361 aagagatgaa tggaagagtg gatggaatag ttttaggagg agacagtaat tttggattgg
     6421 agtcaactgt attggattta acagaagaga ctcctatgat actgagacca ggaagtataa
     6481 ctaaagaagt tttagaggaa cttcttggag aagtaaagct tgacccatca ttaagtaaaa
     6541 aagaagataa tcaaaaagct aaagctcctg gaatgaaata taaacattat tctccaaatg
     6601 cagatgtata tataatatct ggaaaaagag aaaatgtagc aacaaagatt aatgatatga
     6661 taaaatctaa tagagacaaa ggtctaaaga caggtgtaat gtgtatcagt agaaaccgag
     6721 agttttatga tggagaagta atcgatttag gaaactctct tgaagaagtt gcaagtaatc
     6781 tgtttgatgc tcttatagaa atggacaaaa agggcgttga tgtcatatat tcagaagagt
     6841 tccctaagac tggagttgga caagcaatta tgaatagact actgaagtca gcaggataca
     6901 aagtgataaa ggcttaagag ggagttgtaa aatatgaata tacttattgt gtgtacaggt
     6961 aatacttgta gaagccctat ggctgaggct atactaagga aagcaattaa agaatctgga
     7021 agaagtattg aagaatattc gatatcatca gcaggtatat ctacagcaaa tggaatgggt
     7081 gcttctgaaa attctataga agtactaaaa gaaatcggta tagacttatc aaatcataga
     7141 agtaaagtaa ttactaaaaa attaattgat gaatcagata taatactaac aatgactaaa
     7201 tctcataaag aaattttagt acaagcagtt ccaaaatgca aagaaaaagt gtatactttt
     7261 aaagggtttg caaataaaaa tgaggaagat atatctgacc catttggtgg aaatttggat
     7321 atttatagaa gcactatgag agaaataatg tattctgtca acgaaatagt aaaaaaaata
     7381 tgaaacggag gataaacaaa tgaagatagg attaggctgt gaccatggag gatataattt
     7441 aaaaaaagaa ataataagct atttagaaag taaaggtata gaatgtgtag attatggaac
     7501 taataatgct acagattctg ttgattatcc tgtttatgga gaaatagttg caaattcagt
     7561 tataaataaa gaagtagatt atggaatatt atgttgtggt acagggatag gaatatcttt
     7621 agctgctaat aaggttcctg ggataagatg tgcagttgta tcagatgtat tttctgcaaa
     7681 aatgtcaaaa gcacataatg atgcaaatat gttatcatta ggcgaaagag ttttaggaaa
     7741 aggtctagca cttgagatag ttgaagcatg gattaatacg gattttgaag gtgatagaca
     7801 tgctagaaga gtaaacatga ttaaatcaat agaagaaaaa cataataaat aataaaaatt
     7861 acagttttat caggaggaat tatcaaaatg agcaaagtgg tagagacaaa ccatccatta
     7921 atacaacata aattaacttt aatgagagat aaaaatactg gttctaaaga ctttagagag
     7981 cttttaactg aaattgcaat gttaatgggg tatgaaataa caaaagatat accattaaaa
     8041 gatgtagaaa tagaaactcc tattcaaaaa acatcatcta aagtagttgc aggtaaaaaa
     8101 ttagcaataa ttccaatttt aagagcaggt cttggaatgg tagatggatt agtaagctta
     8161 atgccagcag caaaagttgg acatgtagga ctatatagag acccagaaac attaaaacca
     8221 gttgaatatt attgcaagct tcctcaagac ataggagaaa gagatataat agtagttgac
     8281 cctatgcttg caacaggggg ttctgctgta gcagctatag atttacttaa gtctaaagga
     8341 gctaaaagta taaaattagc taatttagta gctgcaccag aaggtatagc agaagtgcaa
     8401 aaatatcatg atgatgttga tatatatgtt gcatcagttg acgaaagatt aaatgaacat
     8461 ggatatataa ttcctggtct tggtgatgct ggagatagat tgtttggaac taaataaaaa
     8521 taaataacta taaatattta ttagggactt tcttatatag tgtatataat tttatgtaag
     8581 aaggttcttt tttatatatt ttttctcaaa ataggaatta tgaattgata aatttctcga
     8641 attgagaata aaaagttttt accaattaaa aaagattgct tattttacaa ggttttatag
     8701 aatagtagtc tattttaatg tggcatgtta gttgcttacg attaagatta taggtattaa
     8761 gtgtattagt taaattgaga attgaatatt aataatatta ttgggagtgg taatacatga
     8821 agaattgtat agcagtaaga ggggctggag atttagcaac agcagttatt cataaattac
     8881 acaacagtgg ttttaaagta gttgcgttag aaatagaaga gccattagct ataagaaggc
     8941 aaatttcttt ttgtgaggct gtttatgaaa aaaaagtaac tgttgaaggt gtagaatgta
     9001 tattttgcaa tactgaaaaa gaaatagaag aagttttatc tcaacataaa gtagccttga
     9061 ttatagaccc aaaaggagaa aaattatcta agttaaatcc aatcgttttg gttgatggaa
     9121 tactcgctaa gaaaaactta ggtacaacta aagatatggc accacttaca atagctttag
     9181 gtcctggatt ttgtgcagga gaagatgtag atgttgttat agagacaatg agaggtcata
     9241 atctaggaag aataataact aatggtagag cagaaaaaaa tacaggagta ccaggagcaa
     9301 taaaaggttt ttctaaagag agagttatgc attcacagta tgatggtata atggaaaatg
     9361 tatgtaagat aggtgatata gttgaaaagg gagacctact ttgttatatt aataattatg
     9421 aaaaaaaaca tgaagttaga gcaacaatat ctggagtcct tagaggttta ttaaaggatg
     9481 gagcaaaggt gaacaaaaat ttaaaaatac tagacataga ccctagaaaa gaagaagtta
     9541 agaatgcttt tacaatatca gataaagcta gatgtatagc tggaggggtt ttagaagcca
     9601 tactaaacaa tgggatttat ccccaaaatt aatttatatt agataaagct attaatattt
     9661 tattaagatg atataataaa tttaatagat taaagagagc atatgacggg tatttaaaat
     9721 ttaaatggta aaaatgctct ttttttattg gacattcaat taacatgtgt atataatata
     9781 aaaataagta tttatttagg ttgaaatcta ataaatatat aataccagta cataagcaaa
     9841 gaggagaaaa gtaaattatg agaccatcat gggatgaata cttcatggaa attgcagaag
     9901 tagtgaaaaa gcgttcaact tgtattagaa gacaagtagg agctgtaatt gtaagagaca
     9961 aacaaatatt aacaactgga tataatggtt ctccgagaaa tcttgaacac tgtgaaaata
    10021 ttggatgtaa gaggcaggaa ttaaatattc cctcaggaga aaggcatgaa ttatgcagag
    10081 cattacatgc tgaacaaaat gcgataatac aagctgctca taatggtatt agtgtagatg
    10141 gggcaacttt gtatgttact acaagacctt gtgttttgtg tgcaaaaatg tgcataaatg
    10201 caggtatatt gaaaattgtt tatgaaggtg attatccaga tgatatgtct acagaattat
    10261 taaaagaagc aggaatagaa ctcataaaat tttagtggat aaaatttata tgacaataaa
    10321 tgtcaaatta tgacactgaa atacaatggt atcaaatgat taaaagaaag tatttgaaaa
    10381 atcatcagat tagtagtaag atatatttta taattaaata aatttatgag atagattaat
    10441 tacatgttat ttattttata ataatgaggg atactattta tgagtaaaag aaatacacat
    10501 acagaaattg caaagttgtt tagtttaata agtcaaatag gattgatgat tgtaatatca
    10561 atattaggat gtactttctt aggaaaattt ttagatagta aattgaatac tactccagtt
    10621 ctgacaataa tatttttatt gttgggtgtt ggaggtgctt ttagtggtgt ttataaaact
    10681 ctaatagtat atacacgaag gaagtgatat attggatata aaattactta accaggtaag
    10741 gtcagtaaca aaaggtatag taatatttga tgtagtttta attgtgctaa tgttgataac
    10801 atcaacatta agtaaaccag tattattagg acttatattt ggctctataa tagctatgtt
    10861 aaattttaga ttattggcga tatcactgga aaaatcagtg acatttcctg ctgggaaggc
    10921 acaagcttat gcatcagcac aatatgctat tagattattt ataatggcag ttgttctaat
    10981 tgcatcagct acagtaccac atttaaatat aatcggaaca gcattgggat tattgagcac
    11041 aaagtttgtc atattatcaa aaaactttat aaataaatta aagaggaagg aggcataagt
    11101 atgaatcaag cgcattatta tctcatagga aactttaaga ttagtgaaac aatactagta
    11161 agttggggaa taatggcctt tttagcgata gcatcttatt ttttgactag aaacttaaaa
    11221 aaagttccaa ctagtaaagt tcaaatattc ttagaatttg cagtaggtgg tcttgctaaa
    11281 atggttacag atgttatggg taaaaataca gtaaaaagaa tgccatatat gataccatac
    11341 ataggaagct tgtttttatt tttcgcatgt tcaaatttaa gcccattact tggattaagg
    11401 tcaccaacaa ccgacttaga cacaactgct gcatgggcaa tgattacatt ttttatgatt
    11461 tattttgcag gagttaaatt taaggggtta tcatacttta aagaattggc tcaaccactt
    11521 gccataatgt taccaatcaa tattataggt gagttagcta gaccaatatc attaagtttc
    11581 cgtccatttg ggaatatttt aggtggaaca ataataatgg gtctgtttta tcaactttta
    11641 ggttttatat ctagtttaat accaggatta tcaataccaa ttggagaaat attaatacca
    11701 gtcccattac acttgtactt tgacttattt gcaggattgt tacaagcata tatatttata
    11761 atgttaacaa tggtatttat atcaagtgca ggagagttag attaagaaat caacttatat
    11821 taagataaaa ataaaataat ttaaaaaatt aaagttttaa ggaggaaata attatggaaa
    11881 cagcaatagt agcggcagca tcagcaatag gagcagggat agcagttgca acaggtatag
    11941 gtgcaggtat aggtcaaggt atagcagcag ctaaagctgc agaagcagta ggaaaccaac
    12001 cagaagctaa aggtgatata acatcaactt tattactagg agttgctata gcagagtctt
    12061 cagcaatata tggtttggtt atatcaataa tacttctatt cgtaaatcca ttctttaaat
    12121 acttaggaat gtagatttag tccttaaata ttttgagata ggtttatagg taaatcatct
    12181 caaacttata atcatgagat gaactttatc atctcgtgat tataagtagt tatgatttat
    12241 ggaaaggagg ataattatgg aaaaagcact agtaggtata acgtgggaat ttgtgttcca
    12301 aattgttaat acttttataa tatttttact tctgagaaag cttttattca agccagtatt
    12361 aaatataatt gaatcaagag aaaatgatat taaaagtgat ttggcagaag gcgaaaaagc
    12421 aaaaaatgaa ggtttagctc tgaaaaaaga atatgagtca aaaatcaatt ttgccaagga
    12481 tgaaggtcaa gaaatcataa aacaagcaac tataagagca gagcagaaat cagatgatat
    12541 agtaaatact gcaaagaaag atgctcttga tataaaagaa aaagcaaaca aagatataga
    12601 acaagaaaga caaaaggtta taaatgaaat caaaaatgat atatctaaca tagcactact
    12661 tgcagcatct aaagttattg aaaaagattt agataaatct aaacatgaag aattgataga
    12721 aaactttata aaagaggtag gggaagctaa atgataaatg taatagcaaa tagatatgcc
    12781 gaagccctat ttcaattagg agaagaagaa aattcaactg atgtcttatt caaagaacta
    12841 gaaaaagtag tagatatgat gactaaagtt agtaaagatt tctacaaagt tttaaaatca
    12901 ccattagttt caaaatcaga aaagaaaaac ttggtagaaa ttatttttag taaggaagta
    12961 agttctaaca taaaaaactt tttaaaagtt ttagtagata aagatagaat atcatattta
    13021 gaagatatcg aattagctta taaagaatta ttaaataaga aaaacaatgt aattgatgga
    13081 gtagcaataa gtgctattcc aatgagtgaa acagatatta aagaacttga agtcaaactt
    13141 tcaaataaat acaacaaaaa tgttactata gaaaatgttg ttgacaagac tatactaggg
    13201 ggagttctag ttagaattgg aaatgaacaa atagatggca ctgtgaaaac tcgtcttgat
    13261 aagatgaaag aaaagctatc tgaagtaata tcttaagaat ggcggtgaac ccatgaactt
    13321 aaaacctgaa gaaataagtt ctataattaa acagcaaata aaaaattatg agaataaagt
    13381 tgagttgaca gatacaggta gtgttttaac agttggggat ggtatagcga gtgtatatgg
    13441 attagaaaaa gctatgtctg gtgagttgct agagttccca ggtgaaatat atggtatggc
    13501 acttaacttg gaagaagaag tagttggagc tgttatcctt ggagatgaca gtgaaataaa
    13561 agaaggcgat atagttaaga gaactggaag aatagttgaa gttccagttg gagaagcttt
    13621 aataggtaga gttgtaaact cactaggtca gccaatagat ggaaaaggac ctatagcgta
    13681 cacaaaaact agaccagttg aatctgaggc acctggtata atagatagaa ggtctgttta
    13741 tgaaccatta caaacaggaa tcaaatcaat agattcaatg ataccaattg gtagaggtca
    13801 aagagagtta ataataggtg acagacaaac tggtaaaaca tctatagtta ttgacactat
    13861 actaaatcaa aaaggaaaag atgttatctg tatatatgta gcaattggac aaaaacgttc
    13921 tactatagct caattagtta gctctttaga aaaaggtgga gctttggatt atacaatagt
    13981 agtttcagca acagcatctg aatcagcacc acttcaatat atagcaccat atgctggagc
    14041 agctatgggt gaagaattta tgtacaatgg gaaacatgta cttatagtgt atgatgattt
    14101 aagtaaacaa gctgttgcat atagagaaat gtcattgtta cttagaagac caccaggacg
    14161 tgaggcatat cctggagatg tattctattt acattcaaga ttacttgaaa gagcagctaa
    14221 gttatctgat gaattgggtg gaggttcaat gactgccttg cctataatag aaactcaagc
    14281 tggtgacgtt tctgcatata taccaacaaa tgttatatct ataacagatg gtcaaatata
    14341 cttacaacca gagttattct attcaggtgt aagaccagca gttgaccctg gtatatcagt
    14401 atcaagggtt ggtggttctg cgcaaattaa agcaatgaaa aaagttgcag gaacattaaa
    14461 acttgcatat tcacaatata gagaacttgc agcattctca cagtttggtt ctgacttaga
    14521 tgaagatact aaaaagagac ttgctcaagg tgaaagaatc gttgagatat taaaacaagg
    14581 tgagcatcaa ccaataaaag ttgaaaatca agttatgata atatatgctg ttataaataa
    14641 tcatttagag gatattccga tagataatat agctagattt gaatcagaat tgtatgcatt
    14701 tgtagataat aattatccgg aaatatcaag aaaaatatta ggtggagaag acttcactca
    14761 tgatttaaca gatgcaataa atgaatttaa agaaaagttt gttgttgaag tataatcctt
    14821 tttaaaaaag gaggtaaatt aattggcagg agctggaatc aaagcaatca aaacacgtat
    14881 gggaactgtt gaaagtacaa agcaaataac taaagcaatg gaattagttg catcttctaa
    14941 gcttagaaaa gcaaaagaaa aggctgaaga ttcaagagaa tattttaata ctttatacaa
    15001 tacagtaaaa gatattgccc aaacaacacg tggaattaga aatgaatttt taaaagaaag
    15061 agaagtaaaa aataaatgtt atgtagtagt agctggagac agaggacttg caggtggata
    15121 caattccaat gtattaaagg aatctgtctc tcatatgaaa ggtaaaaagg aaacagtgat
    15181 tactgttgga aagaaagcac atgaattttt ttctaaaaga aactacaata tagcaaaaga
    15241 tataaatagt gttgaagctt gtggatatga agatgcgaca gaaatttcac aaactattat
    15301 tgacctgtat aaaaaaggtg aagtagatga agtttatatt gcttatacaa aatttaaatc
    15361 tgctttatca caagaagttg aaattttgaa attgcttcca ttagtctttg aaaaagatac
    15421 agacgaacaa ggaatggcag taaaagaaga aaaacaaaat agatcaaaag tacaatatat
    15481 accatcagca gaagcagttt taagttatat tgtacctcag tttatatcag gtacagtata
    15541 tggtggaatt ttggaatctt ttgcatcaga gcaaggtgct agaagaactg caatggaatc
    15601 agccacagac aatgcgaatg aaatgctatc taatttagag ttaagttata acagagcaag
    15661 acagtcagct gtaactcaag aaataacaga gatagttggt ggagttgaag ctctaaaata
    15721 gggaggttag gaaatggcaa atgtaggtaa agtagtacag attgtaggag ctgtactaga
    15781 tgttaagttt gatagtgaac aaagcctacc taacttatta aatgcgttag taataaagtt
    15841 aggagataaa gaaatagttg cagaagttgc tcaacatata ggtgatgata ctgtaagatg
    15901 tatagctatg agtgcaacgg acggacttgt aagaggtatg gaagttgtag atacaggagg
    15961 gccaataagt gtccctgttg gagatgagac tttaggtaga atattcaatg ttcttggaaa
    16021 accagttgat gggaaaccag ctccaaaatc tgcacctaaa ttacctatac atagaccagc
    16081 accggcgtat gatgaactag aaactacagc agagatacta gaaactggta ttaaggtagt
    16141 tgacttacta gctccatatc taaaaggtgg aaaaataggt ctatttggag gagcaggagt
    16201 aggaaagaca gttctaatac aagagcttat aaacaatatt gctaagcaac atggtggtat
    16261 ttctgtattt tcaggagtag gagaaagaac aagagaaggt aacgaccttt atggcgaaat
    16321 gagtgagtct ggagttataa ataaaacagc tctagtattt ggtcaaatga atgaaccacc
    16381 tggagcgaga atgagagttg ctttaactgg acttacaatg gcagaacatt ttagagatga
    16441 gcaaggacaa gacgttttac ttttcgttga taatatattc cgtttcacac aagctggttc
    16501 agaagtttca gcacttctag gacgtatgcc atcagctgtt ggttatcagc caacattagc
    16561 tactgaaatg ggtgcacttc aagagagaat aacatcaact aagaaaggtt caataacatc
    16621 tgttcaagca gtatatgtac cagcagatga cttaactgac ccagcaccag ctacaacatt
    16681 ctctcactta gatgcaaaaa cagttttatc aagacaaata tcatcactgg gtatatatcc
    16741 agcagttgac cctcttgaat ctacatcaag gattcttgac cctagtatag taggaaaaga
    16801 acattatgaa gtagctagag gtgtacaatc aatacttcaa agatataaag aacttcaaga
    16861 tataattgct atacttggta tggatgaatt atctgatgaa gataagttaa tagttgcacg
    16921 tgctagaaag atacaaagat tcttatctca atcattcaca gttgctgagc aatttacagg
    16981 aaatccaggt caatatgttc ctgttaaaga gactgtaaga ggatttaaag aaattcttga
    17041 agggaaacat gatgacttac cagagtcagc attcttattt gtaggaacaa tagaagacgc
    17101 tgttagaaaa gcgaagggaa gtatgtagtc atggccagtg aattttcatt gaaagttgta
    17161 actccaagta atacttttta tgatggagat gttgaaatga tagtagttag aactacaaaa
    17221 ggagacaaag gggtgttgaa aggtcatata ccatttgttt caggtatacc tgtaggaact
    17281 ttaaagataa aaaaagaggg aagttttaaa gaggcgaaaa tagcagaagg attcattaat
    17341 gtaactcaag ataaaactgt catattgaca gaatctgctg agtggattta ataaattaat
    17401 ttagagtata aaaagtcatt ttgaccaaaa aaacttttgg gtaaaatggc ttttttgtgt
    17461 tttggtgtta ataaaataac gataaaattg taagttttta gtgataataa attcacaaaa
    17521 tgtttaaatt tactgccaaa ttatgttgta attaataaga taaagaagat ttagatagct
    17581 tttatgtaag tgaaaaagtc ttcatataag aaatacatat aaaattttta taaaatttaa
    17641 aaaatttgct tagttgtgta gtataataaa taatataata gatttgtata taatattcat
    17701 tacaaataat tttaaaagtt acagttttat tataatgatt attaaattaa gataagtaac
    17761 ataaactaaa taataatgtg tattatcgat agtaaattta taaattcatt attaattatt
    17821 gaaagaagta ttataaattt tctggaggaa caatgaatat atttgatatt ggcgtagata
    17881 taattgaaat tgatagaata agaaaggctg tagacaaaaa taataggttt ttggagaaga
    17941 tatttacaga tagagaaata gagtatttta attctaagaa ttttaaagca gaaagtatag
    18001 caggtaattt cgcagccaag gaagctataa gtaaatctat aggtacagga attagattgt
    18061 ttaattttaa agatatagaa gttctcagaa atgaaatggg aaaaccaata gtaaaaactt
    18121 ataataatct agcaaaaatg tgcatagatt ataatgtttt agaaataaaa gtttcaatat
    18181 cacattcaaa agattatgct atagcaaatg cgataaccat aattaaggac taaggagtga
    18241 ttacattatg atggataaaa cagcaaaaga aataatgaca acagatgtaa tagtagcaaa
    18301 acaagatgat agtatagcag atgtagctaa tatgctaatt gcagaaaaaa taggaggtct
    18361 gccagttgta gattcagaga atagagttgt tggaataatt tctgaaacag atattcttaa
    18421 aaaagaaaaa tatatagaag cacctctcta tataaatcta ttacaaggat tgattttctt
    18481 agatgactta aaaaaagttg aaaaagatat aaaacaagta gctgcttata aggttggaga
    18541 acttatgtct aaagatataa taaaggttca tgaagatgat aagtttgatg atgtagctaa
    18601 tgtaatgatt aaaaaatcaa taaacagagt tcctgtagta gatgatgaca acaaacttaa
    18661 aggtataatt tgtagatatg atataataaa agctttatac aatgaataaa ctgaagtagg
    18721 aggtgtgatg tgagaaaaaa gtggaccata gtatgtatta tgttcttggc actggttata
    18781 attgtaatag gatgccaaaa acgacagtcc acaaaagaag aagtatacaa agattttcaa
    18841 aagcagattt ctgatatgaa ttattacagt tgtaaagctg aagtagaagt agttggaaat
    18901 aaaagcccac acaattatgt tttaattcat acttataaaa aaaccgataa ctataaatta
    18961 gaagtaattt ccccaaaaca tttaaaaggg aaaagtatag aatatcaagg tgataaaata
    19021 ttagttaaaa atccaaagat tagtgatgta gtggagttac ctaatacagg aaaaaataat
    19081 caatatcttt ttgtaggaga ctttataaaa aactatcttc aaaatgagga gatgaaagta
    19141 aagttatcca aaggacattt ggttttagag acttttattc ctggggataa taaatatttt
    19201 aacaagcaag tattatatgt aaatgctgac acaaaaaatc ctgaaaaaat ggaagtgtta
    19261 gataaagagg gagtgccaag atttacagta aaatacaaag attttgaata cagaaactaa
    19321 gggggactaa agacatgcaa aaaataacag tgcctacatg ggcagagata aatctagata
    19381 acttaagatt taacttaaat aatattaaaa atttattaga agaagatatt aagatttgtg
    19441 gagtaataaa agctgatgca tatggacatg gtgcagtaga agttgcaaaa ttgctagaaa
    19501 aagaaaaagt agattactta gcagtagcaa gaactgctga aggaattgaa cttagacaaa
    19561 atggcataac acttcctatt ttgaacttgg gatatactcc agacgaagct tttgaagatt
    19621 ctataaaaaa taaaataact atgacagttt attctttaga aacagcacaa aagataaatg
    19681 aaattgcaaa atctttagga gaaaaagcct gtgttcatgt taaaatagac tcagggatga
    19741 ctagaatagg tttccaacct aatgaggagt cagtacagga aataatagaa ttaaataaat
    19801 tagaatatat cgatttagaa ggtatgttta ctcattttgc tacagctgat gaagtaagta
    19861 aagagtacac ttataaacaa gctaataatt ataaatttat gtctgataaa ttagatgagg
    19921 ctggtgtaaa aatagctata aaacatgtat caaacagtgc agctattatg gattgccctg
    19981 atttaagatt aaatatggta agagcaggaa taatattata tggtcattat ccatctgatg
    20041 atgtatttaa agatagatta gaattaagac cagccatgaa attaaaatca aaaatcggac
    20101 atataaaaca agttgaacca ggtgtaggaa taagttatgg actaaaatac acaactacag
    20161 gtaaagaaac aatagctaca gttccaatag gatacgcaga tggatttact agaatccaaa
    20221 aaaatccaaa ggttcttatt aagggagaag tgtttgatgt agttggtaga atatgtatgg
    20281 atcaaataat ggttagaatt gacaaagata tagacataaa agttggagat gaggttatac
    20341 tatttggaga aggcgaagtt acagctgagc gtatagctaa agacttagga actataaact
    20401 atgaagtgtt atgtatgata tcaagaagag ttgaccgtgt ttatatggaa aataatgagc
    20461 ttgtacaaat aaacagttat ttgctaaaat aaaaccatga tattataatc tgggaggcga
    20521 ttttgtgtac gataagagta aagaaaaaat agaagttact ttacctgact ctctgatttc
    20581 tgaagttgat agtatagttc agatggagaa cagtaacaga gaagattttg caaaagctgc
    20641 ttttcagttc tatataactc agaaaaagaa aattcatatt aaagaatcta tgaaaacggg
    20701 ttatagagaa atgggtcaaa ttaatctgtc actagctgaa ttaggtatga cagttgaaga
    20761 agtatcttct gatgataaaa atgaagggaa gattgcccaa ggtgagtact aattgtgaaa
    20821 taaagcgtgg tgatttatat tacgctgatt taagtccagt tgttggttca gagcagggag
    20881 gcgttagacc tgttctgata atacaaaatg atgtaggtaa taaatatagt cctacagtta
    20941 tagtatcagc tataacatct cagattaata aagccaaatt acctactcat gtagagataa
    21001 gttccaatga atatgggctt aataaagact cagtaattct tttagagcaa attagaacta
    21061 tcgataaaaa gagactgagg gaaaaaattg gttgtctaga tgaaaatatg atgataaaag
    21121 tagatgatgg acttcaaata agtttaggat tgtttacttt ttaaagaggg atttgtcttt
    21181 tattagacaa tccctttttt cttttaaata tctcttaagg tttaatttta atgtactatg
    21241 ttaataaaat taaaaataac gcaaaaaaat agcataaaag tgatataata atatggaatt
    21301 gatataaaat tacgaactca agggaggtaa ttgcatgaga gaccataaag gattaaatga
    21361 gattgcgcgt atcataagaa gagatatagt ttcaatgata catagagcca aatctggaca
    21421 tccaggaggg tcattatcag tagttgaaat acttactgcg ttatactttg atgaaatgaa
    21481 tgtggattct tcaaatccta aaatggagga tagagataga tttgtattat caaaaggaca
    21541 tgctgcacca gcattatatg ctacattggc tgaaaaaggc tattttgata aggaagaact
    21601 gaatggatta aggaagatag gaaggatgtt acaaggtcat ccagacatga aaggcacgcc
    21661 aggtgtggaa atatctactg gttctttagg tcaaggtttt tcagtagcat gtggtatggc
    21721 tatggcatca aagttggata atgctccgtg gaatgtgtat actttgcttg gtgatggtga
    21781 ggttcaagaa ggaattgttt gggaagctgc tatgagtgct gcacattata aacttgataa
    21841 tttaattgct tttttagata ataatggact tcaaatagat ggagatatag aaagcgtaat
    21901 gagtttaggt tcaatagttg ataaatttaa agcttttggt tggaatgtaa ttgagataga
    21961 tggacatgat tttgaccaaa tatttgcggc tcttgatata gctaaatcaa cagttaaaaa
    22021 acctacaatg atagttgcaa aaactataaa aggcaaagga atatctttta tggaaaatca
    22081 agcaggatgg catgggacag ctccaagtga tgaggagcta gaaaaagcct tgcttgaatt
    22141 aggaggtgct gacaatgagt aaaatggcga cgagagaagc atatggtaaa gcattagtaa
    22201 aattaggtca aataaatgac aatgttgtag tattagatgc agatttatca aagtctacaa
    22261 aaacacatga tttttacaag tcatttccag atagattttt taatatgggt atagctgaac
    22321 aaaatttaat aggcgctgct tgtggattat caactgctgg taaaatacct tttgcaagta
    22381 cttttgctat gtttgctacg ggaagggctt ttgagattat aagaaattca gtttgttatc
    22441 caaagctaaa tgtaaaaata tgtgctactc atgcaggtct tactgttgga gaggatggag
    22501 catctcatga aagtgtggaa gatattgcta taatgagagc tataccaaat atgacagttc
    22561 ttgttccagc tgatggagtt gaaacagaaa aaattatttt tgaaatagct aagtataatg
    22621 gacctgttta tgttagatta gggagaagtt cagtaccagt tttatttgat gaagattata
    22681 aatttgaaat aggaaaaggt acagtcttaa gagaaggaaa ggatgtatca attatagctt
    22741 gtggaattat ggtaaatgaa gctctattag cacaagaaaa gttgcaagaa gaaggtataa
    22801 atgctagagt tataaacatg tcctcgataa aacctataga caaagattta atattagaat
    22861 cagcaaaaga aacgaatgct attgtaactg tagaagaaca tagcataatt gggggattag
    22921 gttctgctgt aagtgaggta gtaggagagt cttgtccaac cattgtaaag aaagttggaa
    22981 ttaaagatac atttggagaa tctggtactc caaatgaatt gttaaaaaaa tatgaattaa
    23041 cttgtgatga cataataaag actgttaaag aagctattat tgcaaaaaga atgtaagaga
    23101 tataaattat tcatgtttgt taataatata atatatttaa aagagaggta taaagtgtta
    23161 tattaattta tatctctctt ttttttatat gatatcaata tatcaaaaat gtaataaaat
    23221 attcaaattt ttacaaaaat agagaaaata ttcaaatata tattgactat tcagaaaaat
    23281 atgactaata tataatctat agagaaacta attttatata taatgactac ttagataaaa
    23341 gaatctaaaa aaattatgag aggaggagta gggtataaaa tcagattata agttttaagt
    23401 tattatacta aaaagctata aaacagttat catgtatttt atgttaaagg aaattgtagg
    23461 tagtttatcg aattatttaa tcagaagaaa aaatttcaca tggggaggta actgagatat
    23521 gtcagaaaaa gtaaatgtta aaacagtcat cagtttcgca ggtgcgtatg tagcaacagt
    23581 aatcggttct ggatttgcaa caggacagga aataatgcaa ttcttctctt tttatggttt
    23641 tgcaggtatt attggtggga taatttcaat ggtattattc tcatggatgg gtgcttcagt
    23701 tatgtcaaaa ggtaaagagc ttcaacttaa agaacctatc aaaatttatc gtgtttattg
    23761 tggtaagtat ttagggatat tctttgaatg gtttggacct ttattcttat ttggtgtttt
    23821 cgtagtaatg atttcaggag caggagcaac tttaactgaa tactatggat taaatccatt
    23881 tgtaggtaga gtaggtatgg cagtagtagc attattaact gtttcactag gtcttgataa
    23941 attatcaaag atactaggtg gaatagggcc tataataata atctttacac ttttagttgg
    24001 tggtataagt ttagctaaaa acataggtaa tataggagaa gctgcacaag ttcttagcac
    24061 tgtaaagata gctaaaccag ttccaaatgc ctatctatct ggtgtaatat atactactta
    24121 taacacaatt gttgttatgg cattcttaac tggattagga gcaagtgctg ctaacaaaaa
    24181 agaagctatt tgtggtggta tattaggtgg agtagcactt atggctgcag ctataatgat
    24241 gcacttagct attctttctg atataggaaa cttatattca aaagcaatac catcattatt
    24301 cttagcagat aagatttcac cagtaattgg agttttattc tcaataattc ttatattagg
    24361 tatatataca acagctgtac ctttattatg gtcagtaact aatagatttg tagatgataa
    24421 acatcctaag tttaaattaa taactttaat agcagctata ttaggtctta taggtggctt
    24481 cctaccattt gataagctgg ttggtatttt atatccatat actggatata tgggcgtcat
    24541 aatactggtt tgtgttcttt atagacagat tacaaaaaca tctggatatc aagaaggaat
    24601 cagtgacaaa taagtatttt ttaatattgt aaactcaaga gctatatgaa aacaatgaaa
    24661 atgttttcat atagctcttt ttatttttta gctcgtttat tatgaaatag gaaaaacatg
    24721 ttttttagtt acattacata tatttataat tatggataaa tatatactaa tcaataattt
    24781 agagtctaag ataagcttat aaatcatata tttagaatat atgggaggag tgataagatt
    24841 gaatgtaaaa tttaatatta aaggtataat ttatggagtt atagctcttg tattcgttat
    24901 agggggattt atattcatac caagtatgtt tgtagagaaa tctaaaccca tagattatac
    24961 agtattacaa agaaattcaa taccagaaaa gatattagat gtgatggata agtatacaaa
    25021 tgaagaaaga gcattagcgg ctaaaatcga taacaaaata tacattatag tgactagagg
    25081 aaataataaa tatggtatag aaatggacaa aatagaaagt gtggttgagg aaggaaagga
    25141 tgttttgaga gtcaagataa agtataaaga taaggaagat tcttttccat acatagttgt
    25201 tgaaacaaat atgagtgaac ttccagatag aatagaatta aatgcgacga aataagaagg
    25261 tattttataa ttaaataact gcatattgta caataaagtc atggttttta ttaatgtaag
    25321 taaattgtat atggttctta ttgttgtaaa agccatgact taaaatgctt ataaagtaag
    25381 atattaacag tagtatttat tactatatta aatggggtct ggaaatactt aatgttctaa
    25441 ttatttggag tgataattct agtttcagta actatgtagt ctaattgtgc atcatgagtt
    25501 tcttttggaa tagaatcgaa tatctggaag tcaaatgcta tgccaatagt tatagcatct
    25561 tttcttaagt tttctaaaaa tctatcataa aatcctccac catatcctaa tctatagcaa
    25621 tgtatatcat atgctacagc tggtacaatt acaacatcta aggccttaat atcaattgta
    25681 ggtgattttt cgttaggttc tcttattcca tatgctccaa ttctcaatcc ctcttttaaa
    25741 tctgttattt gagtagggat taatgtatga ttttctttta ttgtgatggg acttgatact
    25801 attttcttta gagacaaaag ttttttgatt aaattatctg tttgaacttc attgttaaaa
    25861 tttagataaa gcattatatt agttgcattt cttatatctt ccatttgaag taatttttca
    25921 gttattaaat ttgaattact aagtacaaag tttgcactct tattttttct tgattcaata
    25981 actttttttc taaattcttt tttcataaaa taattcctcc cttaatatgt tataatgtgt
    26041 acagcaattg tatttaatat aaattttagt ataatttatt gtaataaagc aatatatttg
    26101 ggtaaatgta ataaaaataa cagctagtca tataataata atagtttatg taaaaggaga
    26161 tgtatcttgt gatttcaaag aagaaagcta tttttttagg ggtgatttta gtaattataa
    26221 cagctatggt tacatcagca tttcagttga cattggggaa taaagttgta atatcgaaag
    26281 aactctatga agactataaa aaatatgata agttgttagg actagaatct attataaaac
    26341 aagattttta taaaaaagta tcagatactg atttggtaaa tggagcagca aaaggtctat
    26401 ttctaggaac aaatgataag tattctggtt attatactaa agatgaaatg gagaatctaa
    26461 ttaatgatag tgaaggttct tatgttggtg taggtatgta tataggggca tcaaaagatg
    26521 gtggcttagt agtagttccc atgaaagatt ctccagcaga gaaagcaggt gtaaaatcag
    26581 gggataaatt agtaaaagtc aatggaaaat ctgtctcata taaaaattca gatgaagctg
    26641 ttcgtatgat gaagggtaaa aaagggaaaa cagttgaact gactatatta agagaagata
    26701 aacaattgaa ctttaaagta aaaacagacc agataattga gaagagtata gaaagtaaag
    26761 taattgataa tgatttagga tatatagaaa ttacccaatt tatatcaagt acatatactg
    26821 actttgataa agctttaaaa gaattaaaag caaaaaatat caagggatta gtaatagacc
    26881 ttagaaataa tcctggtgga atgctagata tatgtaaaga agttgctgat gagttaattg
    26941 gtgaaggaac tatagtatat acaaaagata ataagggaaa tactgagtac ttgaaatctg
    27001 ataaagaaaa attgggactt ccaatagtag tgttaactaa tggagaaagt gcttctgctg
    27061 ctgaaatatt aacagctgca atcgttgata ataaagaagg tatttctgta gggactacaa
    27121 cttttggtaa agggttggta caatcagttg taagattgaa ggatggaaca ggatacaagt
    27181 taactactgc tcaatatttt acacctaatg gagattatat caatgaaaaa ggcataaagc
    27241 ctactattga agaaaaagat gaaaataaac agttagatgt agcgactaaa tggcttagag
    27301 aacagattga taaataaagt ttaattttta taaacttata ttgtaaaata aagaacctta
    27361 ataaaaggag tgtctcaaaa ttaacttttt agtttatgag gcactctttt gtgggtcttt
    27421 aaaataaagt gtgtattctt ctaaaaagta gtgataatta aaatattaaa tatcactata
    27481 tataaggaag gtgcacactt tttatgttta acaaaaacga aatattaaaa aaacttattg
    27541 aagaatatga tgttaaaaca actacagata ttcaagatat gctaaaagat ttatttgcaa
    27601 gtacaattca tcaaatgtta gaagcggagc ttgacgacca tttaggttat gatagatatg
    27661 acaataagaa taaaaatact aaaaactcta gaaatagata tagaaataaa aatgttaaat
    27721 cagattttgg agaaataact ttaaatattc caagagatcg aaatggagat tttgaaccaa
    27781 aggtaattag aaattatgaa aatgatatat caggaataga gaaccaagtg attggaatgt
    27841 actcaaaagg tatgtctact cgagatatat caagccacct taagagtatt tacggaattg
    27901 atatatctca ttctctaata gctaaaatta ctgatagagt tctaccttta gctcatgaat
    27961 ggcaaagcag agctcttgat atggtttatc caattgtatt tatgggtgct atacattata
    28021 aagttagaag tgaaggtaga ataataaaca aagctgcata tatagccata ggtgttaact
    28081 tagaaggtat aacggaggtc ttaggaatat ggattggaga aaatgaaagt tcaaagtatt
    28141 ggctaaaggt gcttacagat gtcaaaaaca gaggagttaa ggatatatta ataacttctg
    28201 tagatggatt agttggattt tctgatgcaa ttaaagcagt atttcctgat act
//
LOCUS       DAELXX010000046        27730 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.046, whole genome shotgun sequence.
ACCESSION   DAELXX010000046 DAELXX010000000
VERSION     DAELXX010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27730
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.046"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(150..2030)
                     /locus_tag="KPH29_001885"
     CDS             complement(150..2030)
                     /locus_tag="KPH29_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HBG1467483.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSVTCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRVE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVLGKGTSDKPALK
                     IVTYNNKTVDYENINSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKITKGRKATINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGNGGNNGGGSNSGGSSSGGSSGGGSSSNSTSNVTVKKLKGADRFETA
                     TKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASVKNSPILLSSNEKLPQKTVEELK
                     RLNPSKVIVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAVQFIKSNKVSNIYFIGGI
                     EKISNKAIEQVGKIANKDISNNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVVDKIKSLI
                     EK"
     gene            complement(2240..8203)
                     /locus_tag="KPH29_001886"
     CDS             complement(2240..8203)
                     /locus_tag="KPH29_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HBG1467484.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGN
                     LEVDLNFSLPIKNSESESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNIGTDNIEYTIK
                     KLSASRSEIKNKDENVSYYNIVFNNLPVGKYNIEVSGAGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDEISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDEDGNVQQ
                     IPYENKEYMKNLKDKEQQEEVNNEQEEVNQEEVNNKDENKNQTDNNSNNKDENNDKQQ
                     ENSDKQEVNSNTNQDKNNDKENSSMDKPNSDEDTPKEGDNISKDDNSTTSTYSRAALE
                     TYTDVQNSDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEM
                     NIPKINKISTSTAVGNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTI
                     EVRDIEAYGLYRVSIQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEF
                     QVVPDTKPEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIR
                     GTSLKIEGLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESIDAPTTPNYRLINTPKEN
                     GGFGIPTNHIVDVVYPAGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRG
                     PIITFDKSYTMDTFMATPRFDVSDKTINDFEIRYWDENGQLHNLGDVPFETKTDPVNN
                     NKYVMVRLKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDDLQVEL
                     KEDVTQEKITELKDRLNTPDKASGEYHPYKTVIDRELKLAQDLYNDRATLTDVTTVNQ
                     EINKSGKYKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYT
                     QYYGESGAYKSGEINLKTGKNVIQLSKLHSLDIECGGSLYIRYPEDTPSGGDIKVRVA
                     GATKIPHLNLNGTINDKSPAGVSESKKKIKAYIEELAKYKDAVKGEPYYPGTNIGNSY
                     EYSEKTGVLNTTDIESDKVTLNVPATAVYQGISKGNADLNTQVDRLYNSMLAWEQIMD
                     LVYSERGVFKTQDLDGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHV
                     GVEYGSVPGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLA
                     LLAQTFDDKTHSRLEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGS
                     MLKNNSDADLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFK
                     AWGVEITPELNSIMDSKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSASFSDG
                     IQSGSLVKSNKISIDLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKG
                     NSKAKFTDSISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITN
                     TVSDGDVRTENDSHGPLQNPEIDNIKDNDVSTTYKGKIISKAEWNKDPQKDPDINVEE
                     NPYIIVDMKETLPIVGLKYTKPEAETRKFSLKGLFNFRKNTSTTYNPLTNYKIQVSND
                     KKNWEDVSQGTFEYGQDLVGGGKDQDNEARVTFNKDDKLWTYQARYVKLISNNKSNIE
                     VAELDIIGPPGDNIEIGTMNNADQTRTNGIGKLAEDYVYQSDDPSTSEDETGKIPAGS
                     IIITGEYSGNPAFNLPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKEN
                     FDSLSDKVKAELYRYNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKAR
                     KATRNAIDVSNKKVITVSNKVKGNIVRDK"
     regulatory      complement(8327..8412)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(8584..9987)
                     /locus_tag="KPH29_001887"
     CDS             complement(8584..9987)
                     /locus_tag="KPH29_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HBG1467485.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILIDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALVASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     DNGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKISDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
     gene            complement(10449..12344)
                     /locus_tag="KPH29_001888"
     CDS             complement(10449..12344)
                     /locus_tag="KPH29_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HBG1467486.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKSETISVSSKNHKLVRDLHKVLTDVKDGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
     gene            complement(12970..13113)
                     /gene="scfA"
                     /locus_tag="KPH29_001889"
     CDS             complement(12970..13113)
                     /gene="scfA"
                     /locus_tag="KPH29_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HBG1467487.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            complement(13179..13541)
                     /locus_tag="KPH29_001890"
     CDS             complement(13179..13541)
                     /locus_tag="KPH29_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HBG1467488.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEVGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            complement(13599..13892)
                     /gene="yajC"
                     /locus_tag="KPH29_001891"
     CDS             complement(13599..13892)
                     /gene="yajC"
                     /locus_tag="KPH29_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBG1467489.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            complement(14026..15147)
                     /gene="tgt"
                     /locus_tag="KPH29_001892"
     CDS             complement(14026..15147)
                     /gene="tgt"
                     /locus_tag="KPH29_001892"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBG1467490.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITSIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPQDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            complement(15226..15711)
                     /locus_tag="KPH29_001893"
     CDS             complement(15226..15711)
                     /locus_tag="KPH29_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467491.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            complement(15723..16748)
                     /gene="queA"
                     /locus_tag="KPH29_001894"
     CDS             complement(15723..16748)
                     /gene="queA"
                     /locus_tag="KPH29_001894"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBG1467492.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFLYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGNNVICVGTTSCRTIESACNEDGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            complement(17066..18085)
                     /gene="ruvB"
                     /locus_tag="KPH29_001895"
     CDS             complement(17066..18085)
                     /gene="ruvB"
                     /locus_tag="KPH29_001895"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBG1467493.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            complement(18192..18794)
                     /gene="ruvA"
                     /locus_tag="KPH29_001896"
     CDS             complement(18192..18794)
                     /gene="ruvA"
                     /locus_tag="KPH29_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBG1467494.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASSAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            complement(18787..19296)
                     /gene="ruvC"
                     /locus_tag="KPH29_001897"
     CDS             complement(18787..19296)
                     /gene="ruvC"
                     /locus_tag="KPH29_001897"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBG1467495.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKVIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            complement(19608..20237)
                     /locus_tag="KPH29_001898"
     CDS             complement(19608..20237)
                     /locus_tag="KPH29_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467496.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKNNGVQTSK
                     VVSASANMDEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMNATIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            20440..20763
                     /locus_tag="KPH29_001899"
     CDS             20440..20763
                     /locus_tag="KPH29_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBG1467497.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNRNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            21031..21885
                     /locus_tag="KPH29_001900"
     CDS             21031..21885
                     /locus_tag="KPH29_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467498.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHNIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(22071..23507)
                     /locus_tag="KPH29_001901"
     CDS             complement(22071..23507)
                     /locus_tag="KPH29_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBG1467499.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNELLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPVETNLLKSEVERLSGKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTHGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNSGKEFMFRSAAAI
                     TKVLGGVSDKEMLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISSGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(23578..24960)
                     /locus_tag="KPH29_001902"
     CDS             complement(23578..24960)
                     /locus_tag="KPH29_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG1467500.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAVLIGGWIYGKRFEKYPAYAIDENDREKN
                     LEKGKDSIKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKRYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            complement(25096..26001)
                     /gene="garR"
                     /locus_tag="KPH29_001903"
     CDS             complement(25096..26001)
                     /gene="garR"
                     /locus_tag="KPH29_001903"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBG1467501.1"
                     /translation="MEGYKMKLGFIGLGIMGKPMAKNLLKDGCNLLVYDINKSAVDEL
                     ISCGAKYASVLEMGQECDIVFTILPNGTIVQDILFGMDGLAKTLKEGSIVVDMSSVTP
                     TESILCANKLKDMGLEFIDSPVSGGEPKAIDGTLAFMAGGKEEIYKKVEPFFNIMGSS
                     SILIGDNGSGSVTKLTNQVIVNLTIAAVSEAFVLAAKAGADPEKVYKAIRGGLAGSTI
                     LDAKIPMIMNRDFKPGGKISINLKDIKNVMQTAHNLDVPLPMTSQLLEIMQTLKVHGH
                     LEDDHSGIAQYFEKLAGVEIKKHNN"
     gene            26397..27062
                     /locus_tag="KPH29_001904"
     CDS             26397..27062
                     /locus_tag="KPH29_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG1467502.1"
                     /translation="MATFKRLQEQAYDYLKELILSGEMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEA
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDVYKTTLIHMETPKYINLEDFC
                     NNK"
     gene            27170..27718
                     /locus_tag="KPH29_001905"
     CDS             27170..27718
                     /locus_tag="KPH29_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467503.1"
                     /translation="MIFLNENVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTIEILRKVLFERSEERAIAMRERALENGDELIADNIISTTDVPFLGWVPEFKE
                     LHCPYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDDKVSEGKYTYGNKNCK"
BASE COUNT         8480 a         4974 c         3137 g        11139 t
ORIGIN      
        1 atttaaaatt aaacttaata tagctcaaat tattatacat tttaacctca atattattaa
       61 attgttaaga taatgaaaat aaaataagaa tgcatctaaa tatataactt tatatctaaa
      121 tacattctta ttttcgattt acttaatatt tatttttcaa ttaaactctt aatcttatct
      181 actacagatg aggctattcc tccacctact tcaaacaact ttgatgaatt tttacctttt
      241 aatgatgatt cttgtgattt atctaacgta tttgtagcaa gtataactgg tgaattgttt
      301 ttagctgcta atggtccaac tgctaaagca tctataagtt tatcttgatt tgcaactaca
      361 actccatcta gtttagattg agagtagaat ttatctatta ctttggcatt cgtctcttgt
      421 ctagtttgac ctgccaccct attgtttgat atatctttgt ttgctatttt tccaacttgt
      481 tcaatagcct tgttagatat tttttctatt ccaccaataa agtatatatt tgacacctta
      541 tttgatttaa taaattgtac agcttcatta tctacaccat ctttttgtgt aagtactatt
      601 ggagtttttt ctttaccggc cagtgatgct attgataatg aatctgcttc tccatttcct
      661 gcccctatat acaatttgct tacattattc gtcttatcta tttcttttgc tatattaata
      721 gaagtttgat atcttgtatc tccacctatt ctctgtactg atatttttga atttacagct
      781 ttgatagctt caacaactga atttggcatt gagttgtttc cacctatcac aattactttt
      841 gatggattta gccttttcaa ttcttctaca gttttttgag gaagcttttc atttgatgat
      901 aaaagaatcg gtgagttttt tacactagca agtggtgtag ctgtaagccc atccaccatg
      961 cttttatctt ctccatttac tattacaact gtatctgatt ttgtccaacc actttgagat
     1021 attttggtag ctgtctcaaa tctatcagca ccttttaatt ttttgactgt gacattagat
     1081 gtagaattac ttgaacttcc tcctccagaa cttccaccac tagacgagcc gcctgaatta
     1141 cttccaccac cattatttcc accattgcca ttatctggtg gtaattcatc cggttttgat
     1201 aattcatttt caactatgta ttctaaactt tttggaagtg taacttcaac atatttattt
     1261 attgtagctt ttcttccctt agttattttt tgtgctaatc caagttttaa tattacattt
     1321 gaaccacttt cttgtgtttc tttactaaca atagaatagt ttatcctaat attgtttaat
     1381 aaatattcaa gtgcttcact tttctctacc ttatacttct cagggtcatt tttactagaa
     1441 ccagttccac caattggttt tacttcactc tcattaataa gggtaaatat taatccttca
     1501 tcagagttta tattttcata atccactgtt ttgttgttat atgttacaat ttttaaagct
     1561 ggtttatcgc ttgttccttt tcctaaaact ttatagtcag catttgagtt actgccagat
     1621 ttttttacag atattcttcc tatattcaat ctatttccgt ctagtaaatc ttcatttgat
     1681 gtaacatata tatctaatgt agttgtatta cttgatttgt tgtattctac tctacaagta
     1741 gcatttgcat ttccactttt tatctgagat tttatattat cagaccaagt aatcttttct
     1801 ttctgctcat tatctactac ttcaacctca ccttgtactt ttatactggc ttgaaatgaa
     1861 tatactatgt tttctactga gtccacagat atatctatag tatttacttt ctcatacgtt
     1921 tttgttcctt gtaaaactgg agctgcatat gcacatgtaa cactaccagt tacagctact
     1981 gccatagttg atactgctat agttcttata aaagattttc ttttactcat taacacccgt
     2041 ccttatctat cattaactaa gtggataaat ttacttatcc acctagcttt tatatttaat
     2101 ttataaatta ccctaaactt atagttacat ttatatactt tgcttttaat atctactatt
     2161 ttaaaactat tctagtacct aatcatatta gatatacaat gttagtatta gtatttttac
     2221 gtttttagag ttccacctac tatttatctc taacaatatt accctttact ttattgctta
     2281 cagttataac cttcttatta ctgacatcaa tagcatttct tgtagctttt ctagctttac
     2341 tattactatc agtaagactt attgtcttta actcatcata attcttagct cctacaggta
     2401 catacattgt atcacttaca aatctttgac ctactggcgt atctccttga aggtcattgt
     2461 atctatatag ttcagcctta accttatcac ttaaagaatc aaagttttct tttggcatat
     2521 agtaaaccca tgtaccttta ctgatttcac ctaattcacc tttttctgga acttctgcaa
     2581 ataaaagtgc ttctccagat atagtcttat tgtttttatc tataagtagt ggtaaattaa
     2641 atgctggatt tccactgtat tcacctgtaa taattattga acctgctgga atcttacctg
     2701 tttcatcttc acttgtgcta gggtcatcac tttgatatac ataatcttct gctaactttc
     2761 caattccatt agtacgagtt tggtctgcat tattcattgt gccaatttct atattatcac
     2821 ctggaggtcc tattatatct aattctgcaa cttctatatt agacttattg tttgagatta
     2881 gttttacata tctagcttgg tatgtccaaa gtttatcatc tttattaaat gtaactctag
     2941 cttcattatc ttggtctttt cctccaccaa ctaaatcttg tccatattca aaagttcctt
     3001 gacttacatc ttcccagttc tttttatcat tacttacttg tatcttgtaa ttagttaaag
     3061 gattgtatgt tgtgctagta ttctttctaa agttaaataa tccttttaat gagaacttcc
     3121 tagtttctgc ctctggtttt gtatatttta gtcctactat aggtaatgtt tctttcatgt
     3181 ctactattat atatggattt tcttctacat ttatatcagg gtctttttga gggtctttgt
     3241 tccactctgc tttacttatt atctttcctt tatatgtagt acttacatca ttatctttta
     3301 tattatcaat ttcaggattt tgtaatggtc catgtgagtc attttcagtt ctaacatcac
     3361 cgtcagaaac agtatttgtt atgaatgacc atgctgattt atttattttc ccttcatctg
     3421 ataattttac agtgccaact tcaaattctt ctgttggatt taggtgataa tcatatgcaa
     3481 ctaccttata tgtaaatgct ctgttattaa gaggagatat actatcagta aatttggcct
     3541 tactatttcc ttttgcatcc acaaatccaa ctgttctgta ttttacctgt gttccatttt
     3601 caccatctaa ataatttcca tcacttctta ttatttcgta tcctagtatc ttatctttat
     3661 ccttatctac atctaggtct atagatattt tattagattt aactagagaa ccactttgta
     3721 ttccatcaga aaaactagca cttactttag tatcttttgc catactagct atattttcat
     3781 ttaaaatctt tcttctagct tcgtcattta catattgtat ctttcttgat tctttttcat
     3841 agttcttact atccattata ctgtttaatt ctggtgtaat ttctactccc catgccttaa
     3901 agtaatctct taaatctttt tcaacaacac ttgatgccat tgctactagc atttggtctt
     3961 ttggtaaact attaattggg tcactacttg aaagtgctct gtattttctt gaaatctttg
     4021 catagaatgt atcattactt aaatcagcat ctgagttatt ctttaacatt gaacccgtaa
     4081 aatcatcatc ataagctaga tgtaattgcc ataacattcc aagcctttca aatacattgc
     4141 ttggtatacc taagctattt gaagttacat gctcatatat cttatccatt gttgaacctt
     4201 caagtcttga atgtgtctta tcatcaaagg tctgagcaag taaagcaagt acattgttac
     4261 tagtttcgct atatgtcatc tttccaatat ctgtaacatg tcctatttca tgacctattc
     4321 cccatccaaa taagtttcct cctgtagctt ttccactatc atctatttgg aaaggtactc
     4381 cattcataag ccctggaact gaaccatatt ctactcctac atgtaatcca gaagcataca
     4441 taaatgctcc aataaacatt ctttggtatt ttatgttcat acgagatttt ggagccatat
     4501 catttttagt taagttaaat tcattatcat ctacagttcc attaccatct aaatcttgtg
     4561 ttttaaatac tcctctttct gagtaaacta aatccattat ttgttcccat gcaagcatac
     4621 tattatataa tctatctact tgtgtattta aatcagcatt tccttttgat attccttgat
     4681 atacagctgt tgcaggaaca tttaatgtta ctttatcact ttcaatatct gtagtgttta
     4741 acacaccagt tttttctgaa tattcatatg aattaccaat atttgttcct ggataatatg
     4801 gttctccttt tacagcatct ttatatttag ctaattcttc tatatatgct tttatctttt
     4861 tcttagattc agaaactccc gcaggactct tatcatttat tgttccattt agattaagat
     4921 gtggtatctt agtagctcct gctacacgta cttttatatc tcctccactt ggagtatctt
     4981 cagggtatct tatgtataaa cttcctccac attctatatc taaactatga agtttgctta
     5041 attgaattac attttttcca gttttaagat ttatttctcc actcttatat gcaccagatt
     5101 ctccatagta ctgtgtatat atcaacttag gagtttttcc acttttactt ccaacatata
     5161 ctgttatttc atctcctgct ctagcagcta ctcctaatgc ctgccagtca ttttgcatgc
     5221 ctaagttatt ggaatttaca gtatactgtc cgtttttatc tttatatttt cctgatttat
     5281 ttatttcttg atttacagta gttacatcag taagtgtggc tctatcatta tataagtctt
     5341 gagctaattt taattctcta tcaataactg tcttatatgg atgatattct ccactagctt
     5401 tatctggagt atttaatcta tcttttaatt cagttatttt ttcttgagtt acatcttctt
     5461 ttaattcaac ttgtaggtca tccataaata gatttcttac atcatcttct atactgtcat
     5521 actcataaaa cttaagttct gatatattag acttatatga ataagatgaa caaacactta
     5581 cttgtatctt agtagcagtt attggttctt ttaatctaac cattacatac ttattattat
     5641 ttacagggtc tgtctttgtt tcaaaaggaa catcccccag attgtgtaat tgaccatttt
     5701 catcccaata tcgtatctca aagtcattta ttgtcttatc agaaacatca aaacgaggtg
     5761 tagccataaa agtatccata gtataagatt tatcaaaagt tattattggt cctctattcg
     5821 ttccctcagc tgcattatat gttgggaatg tccaatgtgt tgtgaagtct ccatctacta
     5881 cattgaattt atcaaatgga tttttatcac tatattcact tttgtcatat cctgctggat
     5941 aaacaacatc tacaatgtga tttgttggta ttccaaatcc accattctcc ttaggagtat
     6001 taattaatct atagttaggt gttgttggtg catctatgct ttcagttgta cctatatatg
     6061 ttttagacat tccactagtt cccaagtggt tagtagctgt cattctaact tcatatgttg
     6121 tgttatcctt aagaccttct atttttaaac ttgtcccacg aataagctta tcattgtatt
     6181 ttatccaaga cccattagag cctttttctt tatagtacaa gtcaaagtct ttagcttgtt
     6241 tatgattttt ccagcttaca tcaagagatt taaatttacc tttaacattt ataccttctg
     6301 gtggttctgg ttttgtgtct ggaacaactt gaaattctaa tatgctatct gggctaaatt
     6361 gtataggttt atagtttgag tctacatttt ctggtactcc atctaagttt tctggcgttg
     6421 catctttata accactagac caatctccat ttaaagattg tatgctaacc ctatatagtc
     6481 catatgcttc tatatctcta acttctattg tatttttact agtttgtaat tttttagtag
     6541 ataatacttt tcccttacta tcaattttat ctagtataag ttcgtatgct gatacattta
     6601 cttcattatt ccaataaaaa gatacttttt catttccaac agcagtgcta gtcgatattt
     6661 tatttatttt aggtatgttc atctctggct ttggtatttc tttatataca ttgtttaaaa
     6721 attcaacttt tgatatttct gcaagatttc tatcgtttgc tgttgtagct gttattttta
     6781 tagttatctt ttttactgct acttgtttac caagatttat tactatatcg ctattttgaa
     6841 catctgtata tgtttctaat gctgccctac tatatgtaga tgttgtagag ttatcatctt
     6901 tactgatatt atccccttct tttggagtat cttcatctga attaggttta tccattgatg
     6961 aattttcttt atcattattt ttatcttgat tggtattaga gtttacttct tgtttatcgc
     7021 tattttcctg ttgtttatca ttattttcat ctttattatt tgaattatta tcagtctgat
     7081 ttttattttc atctttatta tttacttctt cttgatttac ttcttcttgt tcgttattta
     7141 cttcctcttg ttgctcctta tcttttaagt ttttcatata ttctttattt tcataaggaa
     7201 tctgttgaac attaccatct tcgtcagtaa cctcaactat tccttttgct ggagcagtac
     7261 cttctggagc ttttatattg atgacttcca tattaagtgc ttctgataaa gctatatcca
     7321 ttactgctgg tttttcctca gatatttcat cattttttga agatattgta actccattat
     7381 tttctccact aaataaattt tctacattac cattaatatc tacatcacta tttcctttta
     7441 attcaacctt acttgtatct actataggat tggtagatac tatactttct aggctttttg
     7501 catcttcact ttgtcctaaa tttttctgta cataatctaa gtcaacaata tctacctttc
     7561 catctctatt taaatcaaat tctcttttat ttgaatctat attctctaaa accttattat
     7621 aatcactctt atctacttta ccgtctccat ttacatcacc cattaagaat aattcataat
     7681 ttaaaggttt atttttatta tctacacttg ctctattgct taataagact ctttgtgaat
     7741 aacttgatat atcaatgtct ttaatttctt tgttcttaaa tccagctcca gagacttcta
     7801 tattgtattt tccaacaggt aaattattaa atacaatatt ataatatgat acattctcat
     7861 ctttattttt tatttcactt ctacttgcag ataatttttt tatagtatat tctatattat
     7921 ctgtcccaat atttactgtt cctgaatctt tttctccaag ctttattatt ccactattgt
     7981 tactatcatc ttttaatcgt aactgcatat tagtcatact ttctgactcg ctatttttta
     8041 ttggcagtga aaaatttaaa tctacttcaa gattccccct taacttttct gtaactgatt
     8101 tttcaactgc ccttagcttc ccctctaata taggttctgc aaaaacattt agtggtgcac
     8161 tacacataga tactgctagt acacaagata ttgccttttt catgaaacaa caatcctccc
     8221 ttaataaatc tctaattttt catgaattta aatactataa gtttcagaca ataaaaaaag
     8281 acactcaaag agaatcttaa ttataaaata aaaagacttt actcttttta gcaactggct
     8341 gatatggctt taaaagcttt aatccctata gttttgcgtc attagatttc cctaattttg
     8401 ccatttaatt ttcactactt tcttacccat tttatactat atttaaactt tttaatacaa
     8461 tttttttaca ttaaattata aaaaaataac atttatatta ataaattaat ataaatgtta
     8521 ttttccatta taatttaatt atttatatag cttccttaca agcataattt tgtgtatatt
     8581 ttcttattct ttatatcttc cataaaattc atgtgtagca tatagattct tatctcctgt
     8641 tatagctata ccaaatccca ttatttcata ccctttatgc aacatattag ttccttcttc
     8701 tggatttgtc ttccactcat taaaaataaa gtttcctata tcatctgcat ctctatttgt
     8761 atatacaggt ttatctgaaa ttttatattt aattatattt tcaccttgaa ctgctatata
     8821 tccaggttta atctcactcc aatctaagta tttcaaaaaa gtagaatatg aattttttcc
     8881 atttatcgta tgagataaag tcttcttatt tgccattaaa ttagaccaat catttgctag
     8941 accctctaat ctgcttgcta cagataacgc tcttactcca ttatcttttc tatactgctt
     9001 tgtaagagca tatactttac cctttattct ctctttaaat gctctatcat ttggattgta
     9061 atataaccct tgttgatttg gtgaagcttg atttatacat tgcttgataa cattctgatt
     9121 tatgcctcct agtacagtca atttgtctgc accacttaaa atactcttgt tactgccatt
     9181 ttcaactaaa actacaggag cattttttgc tattgtagag gcgaccaaag cgtctgttaa
     9241 ttgcaagcct ttacttagat aaaattcttt actattttta taaaactctt gtataactat
     9301 cttattagtt tcaaatctat ctgtgccacc caatctaata gaatttgtgc tcttaactaa
     9361 ttcgtcgctg attatttcac tagaaccaag cgcataactc tttaagcctg tcgtgtcaaa
     9421 tcccacacta tttccatcta taagtattat aggtactcca tctctagcag ctactggtga
     9481 tgcacttatc gcatctgctt ctcctgtaaa tccattggct ataattactt tatctattgg
     9541 cttaatttct ttgatttttt cagctatttt tatacttgta tcaaatctat tttcaccaaa
     9601 tattctttgt acaaccttct ttttggataa taactgattc tcaacatttt tacttattgt
     9661 tgattctgag cctattatat acacagtctt tgctttgttt aatctttcca tagttgtgtt
     9721 tggaatctga ttttgctttg tcattaaaat aggcgcattt aaggctccag ctaaaccact
     9781 tgcacttaga ccatctgcta agctattatc tgagttgatt agtattgctg tattgtagtt
     9841 ttgcttatct gctatttttg ctgctgtctc atatctgtca ttccctttta tttgctcaac
     9901 tgatgtcaac gcatttacca taggagtatt taccattact aaaaatagtg taagtcctat
     9961 agctaatatc tttttactat tcttcatatt attttctacc cccaatgtac ttttcatatt
    10021 ttttattagt actcatactt atttttaatc aaaagttaca attttgttac catattttat
    10081 atatgatacc atagttatcc atacaactca aacaatatac ctaaagtttt tattctctat
    10141 aaattcaaaa cattataatt tattttattt aattaagtta taagttcaaa ataatttatg
    10201 ttagtaatat gtttaatcat tataagaagt atctttttat aataattttt gcaatatgaa
    10261 tttctccaca ttataatcaa aaaataaaat aaggtatctc aatctaattc taaatgagac
    10321 accttatttt tattatttta tcttgttatt tgtatatttt ataatatcta cactatgtct
    10381 tacatattaa cgcattgtat aatcatatat tatctaaata aacttttaat attaattcct
    10441 atatcttatt agttcttagt aaataagttt attttttctg ctattttttc aatagcgttt
    10501 ttagctattc cttcacctat ttgtactaac ttagtagcat tcttaagtct taactcaact
    10561 acttcttctt gtgatttaga aagttcttct gtagttaata ctattggagc ctttgaacta
    10621 gctgcaagtg gtgctgctgt aagtgcatca actaattgtc catttccacc tacataacca
    10681 tcttttgcta catatgcttt agttgcacct gcatagtatt tattgattac tttagcatta
    10741 gtatcttttc tattttctcc agctaatctt tcaactttat tatttaaatc tatatcaact
    10801 aatttgtctt caacttcttt tgatacagaa ttttctccac ccactatttc taattctttt
    10861 ccttttaata atttttcagc atttgcatct aattcgttac ctgtaactat tattggagca
    10921 ccaaattgag cagctctagc tgatatactc atagcatcag cttctccatt tccacctact
    10981 acaaaagcag tcttaaatgt atcttttaat tgttttgcta tttctaaaga agtttcaaat
    11041 ctatcttctc cagaaactct ctcaacttta cttacaccta atttagaaag tttttcttca
    11101 gtttcttttg atactttaga ttctccacct actatatata ttttcttaga agatagatta
    11161 cttccaagta ctcttaatat ttcagcttct atttctgatg gtagtgaatc ttttttagat
    11221 aataatattg gtgcattttt ttgagaggca agtggtgctg atgctaatcc atcaactata
    11281 gcatcttctc caactaaaat tatagcttct gcttctccat ctttgaatct ctctttacta
    11341 acttctactg ctgttgtaaa tcttgaatca ccagataaaa cctctacttt taattctttt
    11401 ccatctttta catcagttaa tactttgtgt aagtctctaa ctaacttatg gtttttactt
    11461 gaaacactta ttgtttctga cttttcattt gccttgctta aaactatatc aaagcttgat
    11521 ttttctggtt tatctttatc atcactatct tcttgttctt gagtaaggat tgcttcattt
    11581 tcaaatgtta aagcatatcc atttttaact ttattaacta tttctcttcc taatgtagtt
    11641 aatctaattc catcaaataa ttcttctgct gatatgtctt gtaaatctgc gtttttaact
    11701 gttatcgtat gtaattcttt tgaatctatg tcactataac tcttttcata accttcaaaa
    11761 gtaccttctt ctgatgttat cacttttgag aaatctaaat gttggtctcc tacatttaat
    11821 gtaataacat ttgctgaatc ttttgctctt tttacttcaa ctgtatttgt agattttata
    11881 gttgctgtta acttgttatc agaatcttct gctaaagttg cattgtaagc attaactgca
    11941 tctactattt cttgagcagt tttgtagttt tcaattttat agtcaactac ttttccacct
    12001 aacacttgat gtcctttatc ttgaatagta acttttaaac tttgcccttc tgttaaagag
    12061 tttattttat tttgtaattg agttgcatta gttaagttta cattgtctac ttttatatcg
    12121 taaactctat catttacatt tgctcctgct tttgtatctt caaacttaac ttctaatgct
    12181 tttctaacct cttcgattag tttagcagaa tctttcgcac ttactgtgta atttttatca
    12241 acattttctg tcacagtatt gtctgcgaat attggtgctg ctgcactcat aactgtagca
    12301 cctgccatta ctattgccat acttctcttt ttgttgctta acatatttat gtcctccgaa
    12361 tattattttt tattttttat attcgtattt cccacacata aataatatta tatataatat
    12421 tatttttcaa tatattttat gtgtttacat aatttactaa cacaaacact aataatatct
    12481 tcttgaaccc cttcccccta cttagctctc tataattatg ctcaaagata gaaatcccta
    12541 ttttatagtg attttctact aaacaatcat atactaacaa aagtattcaa actccatttt
    12601 tatatactac tatgcactaa tttttgcttt gctcttatta cttaaatata tatcactaaa
    12661 ttaatatcta cctccatata ttaaaatata taacatatta taaaatcatc ataaattaga
    12721 gtttaaaatt aaaaattatt ctaatacatg taattgataa aaaataaaat ataagagtag
    12781 ctactttaaa ataaatgctc ttatatgatt agatattttt gcataattaa acgataagaa
    12841 atcaaattta attttacata agtagaatat atatttttat ataataattg aaaataaaag
    12901 aggatacctc ataatgataa aagaatcact ttgagatacc ctcttacaaa caactaaatc
    12961 aataattttc tatctttcac actcttgatt tgcaactgta caagaagttt tacaagctga
    13021 ttgacaagaa gtttgacact ctccacatcc accttttgca gcactttgct ttaaagtagc
    13081 ttgagataaa gtttttacat gtttattttc cattttcaac gcctccattt ctaattgttt
    13141 cctattgatt atatcataaa actactttaa tataaactct atttaaagtt gacacccaat
    13201 acgcctccaa tgcctcctgc tgctgatatt agcaataact tataaatcat ccctacttca
    13261 aatacaaact tttcttgtgc taaaaacaca gtcaaaaaaa tacatactat gtacaaaagt
    13321 cctactaaaa gtccatacat aagtcctttt tctttcatgt gttttgaagt gtaaaaccct
    13381 cctatagctg cagaaatagt agttataaat gatgagacca tagttatatt atctccacca
    13441 atatctgtaa atgtcaaaaa taagttatac actaataaaa cagccaaagt tattatatat
    13501 gcatatccta gacctttaaa aatataatta gattttccca taaaaaaacc tcctctcaaa
    13561 ttaatatata ttcgagaaaa ggcttttaaa ttactaattt agtcttcttc tatttcttct
    13621 tttttagatt taacagttcc tatagcccat ttttcaaatg gaactttagt tctatttggt
    13681 cctaattcta atattacaac atcatcttca actttagcta cattagctac tattccacct
    13741 attgtaatga ccttgtctcc tacacttagg ctacttctca tttcttttaa ttgtttgtct
    13801 tttttctttt gaggtctaat cattaggaaa taaaagattg cgattactac tacccataga
    13861 cctatactca taattatttg ttgctgtggc attaattttt cactcctttg taaattatac
    13921 tcttttgtaa atcataataa atgtttatat aacaatttac tttctctatc tttgagtact
    13981 attgaatatt atatattaaa atgattataa ttaatagtgc aaaacctata tttcatatcc
    14041 atattttgca aaaaattcat ttctaaaatc aagtaatcta tcttccatta tcgcttgtct
    14101 tatttgtttc attaaattta ataaaaagtg aagattatga gtagttatta atcttgcacc
    14161 cagtatttca tttgctttta taaggtgtct tatataagct cttgaatagt ttttacaagc
    14221 ataacaatca cattcagggt caagtggagt aaagtcttca gcataagttg catttctaac
    14281 tactacttta ccttggctag tcattgcagt tccatttcta gcgatacgag taggtaggac
    14341 acaatcaaac atgtctactc ctcttataac accttccact aagtcatctg gactaccaac
    14401 tcccattagg tatcttggtt tatcctgtgg cataagtgga gttgtatgct ctagcacgtc
    14461 atacataagt ggttttggtt ctccaacact aagcccacca atagcatatc ctggtaaatc
    14521 aatactagtt atttcttttg ctgattgttc tcttaagtct ttatacatcc caccttgtat
    14581 tataccaaat aaagcttgtt tgtcggtatt attatgggca tctttacatc tttttaacca
    14641 tcttgttgtt ctttctaaag aattttttac atattctcta tctgatgggt atggtgcaca
    14701 ctcatcaaac gccatcatta tgtctgaacc caatgcattt tgtatttcca tagctttttc
    14761 tggagttaga aaatgttttg aaccatctaa gtgtgaccta aattctacac cttcttcttt
    14821 tatctttctt aaaggcccta agctaaatac ttggaaccct ccactatctg taagtattgg
    14881 tttatcccag ttcataaact tatgaagacc accagctctt tttataagtt catgacctgg
    14941 tctcatatat aaatggtatg tgttactaag tattatttgt gaaccaatct cttttaattc
    15001 ttctggtgtc atagatttta cagttgcttg agttccaact ggcatgaata ttggtgtttc
    15061 aataatccca tgtggagtgt gtaatcttcc taaccttgca ccactctgct tacaagtctt
    15121 tatcaattca tatcttactg cgtacataaa gttctcctta accatattta tttctcatta
    15181 tttcttaaga tatatttgta ataagctatt catataattt attgtctaaa tagatttatc
    15241 ttcgtcttct ttgttctgtt gctccttaat tgcttcttca tcatgaatac tcattatatt
    15301 aagagcactc ttgtcttttg ttactaaaaa cttttttata gcaaatagtc ctgctatagc
    15361 tataactcct attaaaacat ctatcatacc ttcattttta ggcagtagca acatttttct
    15421 agctattgcg taaagtaatg cctcaataaa tgctcctggt atatgaagag atagcattac
    15481 tacaagttca acacctatta ctaaaagtaa tatttgtgct aaaaaatcat taagctgact
    15541 gtattgtact ggattttgaa aatcaactat atatgctccc cacataagtc ttagaacatc
    15601 tatagttcct aaaaatactg ctattagaac tactactgct aaaacacgtt caaatataaa
    15661 agcaatcttt aatatagggt tattcttttt ttgtttttgt aaactatgca ttcattacct
    15721 ccctacttta taatcatagc atctccaaaa ctgaagaatc tatatctttc ttttactgcc
    15781 tcattatatg catttaaaac attatccttt ccacaaatag cacttactaa cattattagt
    15841 gtagattctg gtaaatgaaa attagtaata agtttatcta aaactttaaa tttataacct
    15901 ggatatataa atatttctgt ccatccacta gtttctttca tctttccatc ctcattacaa
    15961 gctgattcta tagttctaca actagtagta ccaacacata ttacattgtt tccattttcc
    16021 ttcgctctat ttattttgtc agctgcttct tggctaacca tataatattc tgaatgcatc
    16081 ttgtgattta aaacatcttc tacttttact ggtctaaaag tgcctaaacc aacgtgtaga
    16141 gttacaaaag ctatatctac acctttttct tttattttat ttaacaattc ttctgtaaaa
    16201 tgtaatcctg ctgtaggtgc tgctgctgaa ccattatgct ttgaataaac agtctggtat
    16261 ctttcttttt catctagtct agcagtgata tatggaggta gtggcatatt tccaagttca
    16321 tccaatattt cttcaaaaat tccatcataa aggaatttta taattcttga accttcatct
    16381 gataaatcta caacttctcc aattagcttt ccatttccaa aagaaaattt agtccctact
    16441 tttgctcttt ttcctggttt tactaaagcc tgccaagtat cttcctctgt tctttttaat
    16501 aataaaaact ctattttacc tccagtttca agcttctctc ctattaatct agctggtata
    16561 actctagtat tatttaatac taaacaatct cctggattta aatattctat tatatctcta
    16621 aatactttat gttcaatatt acctgtctct ttatctaata ccatcaatct tgagctagct
    16681 ctatcttcta taggaacttg agctataagt tcctgtggta agtcaaattt aaaatcactt
    16741 gttttcaatg ttaaacttcc tctcttaaaa tttctccagc tacaataata tcaaaaaaac
    16801 aacttatttt ccacaatatt accataatct atcaagaaat tgctattttt aacagacacc
    16861 atatccacaa tatatagata tttttccacg atatacatgt cttatccaca atatatagat
    16921 acaatacaca caatttttat cgtgtttatc cacagtatct acagatataa ttaccatttt
    16981 gtggatatat ttgaattttt agccatttta gaaatatttt aatttaatga ctcttttatt
    17041 ttataaatac tcgtataatc tatttttatt tttcattagg atatggaatt tttaagtgtt
    17101 catatgctaa tggcatggct actcttcctc taggacctct atttataaat cctaattgaa
    17161 gcaaatatgg ctcatataca tcttctattg tatttctatc ttctccaata gaagctgcca
    17221 atgtatctaa acccactggt cctcctctaa atttttctat tatagtcata agaagcttct
    17281 catctacaaa atcgagaccc aaactatcga ctcctaaaag ttccagagca tcttttgcca
    17341 ccttatcagt tatttttcca tcagctctga cttgtgcaaa atctctaact ctttttagta
    17401 atctatttgc tatccttgga gtccctcttg aacgttttgc aagttctaat gctccattag
    17461 actgtatctc tgcatctaag atgcttgaag acctaagtac tatttttgaa agttcatcaa
    17521 ctgtataata atccaattta caaataacac caaatctatc tctaagtgga tttgtaagca
    17581 taccagctct tgtagtagct ccaataagtg taaatttagg aaggtcaagt cttatgcttc
    17641 tagcagatgg ccctttccct attattatat ctaggcaaaa atcctccatt gctggatata
    17701 aaacttcttc aacacttcta ttgattctat gaatctcatc tataaataaa acatcatttt
    17761 catttaaatt cgtaagtatg gctgccaaat ctccagctct ttctattgcg ggtcctgacg
    17821 taattcttaa atttaccccc atctcatttg ctattatact tgctagtgta gtttttccaa
    17881 gacctggtgg tccatataaa agcacatggt ctaactgctc atttctcgat ttagctgctt
    17941 ctataaatat actaagttgt tctttggatt tttcttgacc caaataatct tctaaagttt
    18001 ttggtcttaa actgttttct atatctatat cttccatctt cattgtagaa gttattatcc
    18061 tattttcatc ttcaaaacct tgcatttata cccctcctct cgtacttttg aaaatctact
    18121 ttatatattt tcaaatttac ataaactcct taaatattgt tttaaaatcc aaatattata
    18181 aaatccaaat actatttcaa ggatttactc attatatatg taagagcttt ctttataata
    18241 gcttctgtat tcataccatc ttttttacat ttttgaactg cctcttttga ttccgacaaa
    18301 gtatagccta aagctacaag tgcatctaca gactcatctt gtgatatcaa ggtaggtttt
    18361 tgtgataata acgttggttc aaactctatg ttatttttgt ctaccttatc cttaagctct
    18421 aagacgattc tttctgctgt ctttttacca acaccaggag ctttggataa ttttccaatg
    18481 tcttcactta aaatatatgc tcctaactgt gctgatgatg caaatgatag tattgaaagc
    18541 gccaccttag gtcctatttt agatacagat gttagtaatt caaacatctt aagttcttcc
    18601 ctacttacaa aaccacaaag actcatatca tcttctctta caattaattt agtatatatt
    18661 tttgtggctt ctcccacttg taaattcatt attgtattgg atgatacatt tattttatat
    18721 cctatcccat tattttcaac aactatactg tctatataaa tttcttctac tgtacctttt
    18781 atataactat acatacttac cacctatatt ctttagagtt ttttcaagtt tgtttgaatg
    18841 agcatggcaa atagccacag ctaaagcatc tgcaacatca tctggttttg gaactttttt
    18901 tagacttaaa aatgaagtta ccatctgttg aacttgtgcc ttatctgctc tcccatatcc
    18961 aactacacct tgttttactt gaagtggagt gtattcatat acaggcttcc cattatgagc
    19021 acatgcaagc atagtaacac ctctagcttg tgcaactgtt atagctgttt ttacattctt
    19081 gttaaagaat aattcttcca ttccaacttc atctatattg taattcttta ctattgtatc
    19141 aattccttta tacacaagtt ccaatcttct cgatatattc atatgggcag gtgttgtaac
    19201 tgctccataa tcgattactt taaacttgct atttttgtat tcaattatac catatccaac
    19261 tatggctata cctgggtcaa tccccaatat tatcattttt taaaattccc cctaaaaata
    19321 aaagaaatac caaaatgtat ttctcataaa aacttatgtt ctaaatcatt atatcataaa
    19381 taatattaac aaatcataaa atgctctatg taaaatataa aatcaaataa aaaagttata
    19441 tcaaagcaag attttacctc aatataactt ttatatttat tacaaataaa ttattgtatt
    19501 tttataggaa ttaaatgtga ctacaatttt aatactaatc tatttaatat taaattattt
    19561 attattaact tacttagtat ttaacttgaa ataatattaa tttactatta acttgcaaag
    19621 tcttctaatc ttgaataggc ctcttcttca gtatccataa ctacaccttt tttgatttca
    19681 tcttgaactg ttttaggaag tgaatctact cttatttttg ttttttctac taatgtttta
    19741 cttccactat catcatattt atatagtgct atatttccat tatcattttc aatagtatat
    19801 ttacctttta tagtagcgtt cattgcttgt acatctttat tatcactata tttagattct
    19861 accaatatga tttcattttt atccatactc tttatttttt tagttggata ttctttttta
    19921 aaatatgata ctatttctga ttttgtctta tctaataatg actctggaac tatcccagcc
    19981 attgtaagtg ttctcttata gttagtttca ttttcgcctt cactttgaag ccaaacttca
    20041 caatttttat ttaatccaaa agcttctttt gacttttcgt ccatattcgc actagcactt
    20101 acaactttac tagtttgaac tccattattt tttaatcttg tccctatata aactccgcta
    20161 cttaatacaa atactccaac cagtattaag gcagatattt tccaaggaaa taattctttt
    20221 ttcttctttt caaacattga ttacgcctcc tttttctatt attttctcca agctgtaagt
    20281 ttttatacaa tttaggtaaa ttttagaaga aaatatattt atatttcaaa ttactctaag
    20341 aatagatatt ttctaaaagg tagttttctc ttaatggtta tattcttgta attatatgga
    20401 cataatttat aataaataat cgtttaggag gtaataactt tggaaagaat aaactgtaat
    20461 gtaattaact gttcacataa caacagccat atatgttatg cagataaaat ttttataaat
    20521 ggtgaaaaag ctcacaatga cagacatact tgttgtagtt catttttaga tgaatctatt
    20581 tatagtaatt tatctagcaa cgttaataat agaaattgtg aggaattagt ttgtactgta
    20641 aaaaattgta aatacaacaa agatggtagc ttatgtacac ttgataatat attggttgct
    20701 cctgaaactg atagagtaaa cctttatatt gaaacatgtt gttctagttt cgaatgtaaa
    20761 tagtcatagt ataaatataa gggtgtcaaa tgtaattaaa ctaacatagt gtagagtatc
    20821 tacaactatt agtttgatta tacttgacac ccttatttat tttaaataaa atttcattat
    20881 tataaccatg tttataatgt tctaactgta attaaatttg ataaatttct ataaattggt
    20941 ataatcaata tgtaaattga atttagcaaa ttaatcataa aaataaaata tcgagtatat
    21001 agtgttatac taactagaaa tgaggtgctt atgagtacat tttatattca atttctttat
    21061 tttgtaattt atagctttat aggatggtgt tgtgaaacta catattgttc agttttacaa
    21121 aaacaatttg taaatagagg ttttttaaat ggtccatttt gccctatata tggatttgga
    21181 gcattagcaa tagttgctac cttgactcct tttgtacaca atattccttt gttatttttg
    21241 tttagtataa ttataactag cataatggag tattgtacga gctttatttt agaaaaaata
    21301 tttaatatga cttggtggga ttattctaaa cataaattta atattcatgg aagagtttgt
    21361 ctggaaaact cacttatgtt tggtgtatta tcattgattg taatgcttat agtacatcct
    21421 attgtggttg actttattaa ctctatctct aaaaatgtgc tatttatatt tgctatatct
    21481 atagaaattt attttgtttt agatttagtt atcacagtcc atacaatttt acaattaaat
    21541 ggtaagttaa aacaaatcaa tcttataatt aaggaattaa aagataagaa agaatactac
    21601 aaacttatta cacaagaaac tatagaaaac aaacttgaat ctttagttga aaatagactg
    21661 gatactttgg aagatagatt tgaaatactt aaggaaaaag aaaactatcg ctatgctaaa
    21721 aatcgtattg atgagctaaa aaataaactt aatactttgc tgtctaacca caaattactt
    21781 catagacgta taatcaaagc attccccaac ataagctcta ataagcatac agatatttta
    21841 aataaaatca aagaaaatat taaatcaatt aaaaaagata aataataaaa taaacacata
    21901 agttaaataa taaaaatact aatacataag attaaggttt agaatatttt ttgtaaaata
    21961 ctctaaaccc taattaatat actattattt ttaatcttaa tttataattt tagtttattt
    22021 tacttttaac acagtaaatt taagcgttta aacttcaata cactaatata ctaccccatc
    22081 aatatctcaa ctgattctct aagtgtgcta atctctccca catttccagg gaaaattata
    22141 tatggcatat taggaaattt gctttctttg cctgtcatcc atactggtat accaggtttt
    22201 atctgtccca ttactgtagc tttttttact cttagtgctt tagtaccaac atcactagaa
    22261 gtaattccac ccttagctat aataaaattt ggtcttacat taagcatacc aattatactg
    22321 gtaactgcat ctgatatttc tacagaaatc ataagctgtt tatctttatc attcgtatcc
    22381 aagtcaaaac gctctcgtct agtataaaca gcaacagttt taccactgct tattttttcc
    22441 tctacaactt ttataactct ttttacctca ttttttagac cattttcctg aagaactaaa
    22501 tgttgattaa attctataaa ttctattgga tatttacaat ttttaagttc ttctaactgt
    22561 tgggtagtct tattcacatg tgaaccaaca agtataattc ctccattggt attacctttt
    22621 gatactaact catccttagt aagcatttcc ttatcactca ctccgccaag tacttttgtt
    22681 atggcagcag cgcttctaaa cataaattcc ttaccactat ttactgctcg tataaacgca
    22741 attgtaaata cctttacatc tacataatcg gttgcattta caataatttt attgaatcct
    22801 tcagcttttt taagtttttc tgttatagct tcaatattta gctctctaag ttctttagaa
    22861 gaaatatata tcatatcact agatttgtac tttccatgtg tcttttcttc acaccactca
    22921 cctatatcag atgatttata tccaaaagat ttatcctttg caaattcagt cattcctgct
    22981 ggtacaagta tttctccttc tttaacataa tgtacattat ctatagtaaa cctaccacct
    23041 tctttaaaaa atggcattat tatttcaccc tgaaataact tccccgataa tctctctact
    23101 tcgcttttta ataaattagt ttcaacagga taatgtcctc tcatagtaga atcactacga
    23161 cttatgatta taaaatcctt attaactttc tttgatacat ccacaatatt ctttgaaatt
    23221 tctttatgtg cttttgtagt ttgagcaaca gtaaatcccc ttgaattagt aagtataaaa
    23281 aacatactat ttttctcatt aaaaccttgt tccatactat ccttatccca atcagtataa
    23341 acagaaatat catgaactgt ttgtacacct gttgggtcat catctaatac aacaattttt
    23401 ctatctaaat gcttaagctc atctgcaaga agttcattta atctgtcctc atctacttgt
    23461 ttaaaagtat taagtatatc tatgctaatt ggttttactt ctctcattta aaacgcccct
    23521 ttatgtaatt tgtatagtct aaatgtaatt tatataatat aaacaatagt ttgttattta
    23581 tagacctgga agtggtataa ttccatttat aacactaagc aataaaacca ttataaatgc
    23641 tacaactcca gcaatggttc ctccaattgt ccaagagttt aaagtatctt taactgataa
    23701 tccactaaat cttgaaagta cccagaatcc agaatcattt ggaagggaga aaccaactcc
    23761 tccagcacat atagcaagcc ctactaaaac tggtgacatt ccagttgcca aaattgtagg
    23821 tcctaaaatt gatgatgttg ttactaaggc tacagtagca gaaccttgag aaattctaag
    23881 caaagcactt aataagaatc caagcactac aactgatata ctaagaccag acatagtatc
    23941 aactaagaag ttacctatac cacttgcatt tataacactg ccaaacgcac cacctgaacc
    24001 tgttatcaat aatattagcc ctgctgaatc agcagcttca ataactacat ctcccatagg
    24061 tttatttatg tatctcttta aaaatcctat tgccaccaat attccaagaa gtattgctat
    24121 gtttttatct cctataaatg caagtactcc actcattgta gaaccttttt ctaagaacat
    24181 actaaataca tttgatataa gtataagaag tattgggaac aataaagaaa acatagaaat
    24241 tccaaaacct ggtactttag aagaatcatc atcttcaatt tttatgctat cttttccctt
    24301 ttctaaattt ttttctctat cattctcatc aatagcataa gctggatatt tttcaaaacg
    24361 ttttccataa atccatccac ctataagaac tgctggaaaa gcaacaatta tagaataaag
    24421 tataaaacta cctacatttg catttacatt tgctgcaact gctaaaggtc ctggtgtagg
    24481 tattacaagt gcatgaccta ctatagtccc aactcctaag gcacatacaa aaaccattag
    24541 agatttctta gttactctcg aaacatactt aattataggc atcattataa caaaagctgc
    24601 atccataaaa actggaattg aaattaaaaa acctgctgta gttactgcta aagcactgtt
    24661 tttttctcca gtcttagcta acaacccctt tgcaatagat tctgttgctc ttgattctga
    24721 aagaatattt ccaaatatta ttccaagtcc aattacaata cctatactac ctaatgtatt
    24781 tccaaatcca gtcgatattg tggttgatat ttcatttata ggcattctaa ccataaagcc
    24841 tgtaattata gatgttatca gtaatgccaa aaacggattt actttaaatt taataatcga
    24901 tacaagtaaa attcctatag atataacaaa tgctatcaaa agtccatatc ctgtaatcat
    24961 aatctttacc cctttatcat tctactttat tatttatcaa ttttattttt cttttactat
    25021 gataacttaa cctcgctgta tttatataat ctaatcctaa taatatgtat ttaattatat
    25081 tgatatattt aaatattaat tattgtgttt ttttatttca acacctgcca acttttcaaa
    25141 atactgtgct attccactat ggtcatcttc caaatgtcca tgtactttta atgtctgcat
    25201 aatttctaat aattgacttg tcataggtag cggtacatct aaattgtgtg ctgtttgcat
    25261 tacattctta atatctttca aattaataga tatttttcca cctggtttaa aatcacgatt
    25321 cataatcatt ggtatcttcg catccaatat tgtactacca gctaggcctc ctcttattgc
    25381 cttatatact ttttctgggt ctgcacctgc ttttgctgca agtacaaatg cctcagaaac
    25441 tgctgctatc gtcaaattaa ctattacttg gtttgtaagt tttgtaacac ttccactacc
    25501 attatctcca attaaaatag atgagcttcc cattatatta aaaaatggct ctactttctt
    25561 ataaatttct tccttaccac cagccataaa agcaagtgta ccatcaatcg cttttggctc
    25621 ccctccactt actggtgagt ctataaattc taagcccata tcttttaatt tatttgcaca
    25681 caatattgat tctgtaggag taactgaact catatcgact actatacttc cttcttttaa
    25741 agtttttgcc aatccatcca tcccaaataa tatatcctga acaatagttc catttggaag
    25801 gattgtaaat acaatatcac attcttgacc catttctaaa acacttgcgt attttgctcc
    25861 acatgatata agttcatcca cagcagattt attaatgtca tacaccaaaa gattacatcc
    25921 atcttttaat aaattttttg ccattggctt tcccattatt cctaatccaa taaatcccaa
    25981 tttcatttta taaccctcca tatcttcaca tttttgaaaa cgttttttat gttttagagt
    26041 atactacata ccagtatacc ggtcaatata aaatataaaa gtatttattt taacaataaa
    26101 tagtaatttt taaagtcatt tattatattt agtcatccta tataagtttt aatacatact
    26161 attgttttta aatataatat gctactttta cagttttgta tttttactaa tttcaattag
    26221 ttttaattaa tctgccttta atgttaatca tacttcacac ttcaataatt ttttatattt
    26281 tttaatctta tctgcaattt aaattgcaaa agattgacct tattttcact atgttgtatg
    26341 cttaaaatta tataagtttt tgtattttta tattgtttag gggggtatta ttaagtatgg
    26401 ctacatttaa aagattacaa gaacaagcat atgactactt aaaagaattg atactatctg
    26461 gagaaatgat agaaaatgaa atatattctg aaacaaaact tgctagtgaa ataggtatct
    26521 ctagaacacc tataagagat gctcttcaaa gacttagtca agatggattt atagatataa
    26581 tcccaagcaa aggatttaga atccatcaaa ttacagcaaa tgaaattgta gagatattcc
    26641 aaatacgctc tgcaatagaa gggttttgta cttttttaat tacaagtcaa tacaaagaag
    26701 ctagggcagt agaaactata agtaaattaa aacatctttt agataaacaa aaagatattt
    26761 tactaggtga taaaaattta aactcatttg cagaatacga tacattattt cacacaacta
    26821 tagtttcata tgctcaaaat actgaatttg acaaaatgtt taacaactat atgtacagaa
    26881 taaaaaaatt agctctcgat tcattatcac atgaaggaag gcttgaaaca acattaaaag
    26941 aacacacaga tatttttaat aatattgcaa atggatgtat tgaagatgtt tacaaaacta
    27001 ctctcattca tatggaaact cctaaataca ttaatttaga agatttttgt aacaataagt
    27061 gattttagta ttatactttc atttattaaa aatatatcta cctcaaatta atttcctaat
    27121 ttataaaaaa tattctataa ttatattgta actaatacta ttctaaggag tgattttttt
    27181 gaacgaaaat gttgtaacta atgaaactat tgacaaagat tatgctattg aagaagttcg
    27241 tatggcatgc aaacattttg gtgacttata tttttatttt tccaaagtgt tatttgaaga
    27301 gtttggtgaa gataaaacta tcgaaatact aagaaaagtt ttatttgaaa ggtctgaaga
    27361 aagagctatt gctatgagag aaagagctct tgaaaatgga gatgaattaa ttgcagacaa
    27421 tattattagc acaactgatg ttccatttct aggatgggtt cctgaattta aagagttaca
    27481 ttgcccttat ggagcttcct ggcttttaag atttgaggaa aacccttggt ttaaaaaatt
    27541 cgcttctctt tactgtgatg ttacagatac tactgtagct gaagtattta caggagatac
    27601 gtcccataaa ataacaaaaa acattctatg gggagataaa agttgtgaaa gaatatattt
    27661 ccatgatgac aaggtatctg aaggtaaata tacttatgga aataaaaact gtaaataaat
    27721 aatttaaatt
//
LOCUS       DAELXX010000047        27696 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.047, whole genome shotgun sequence.
ACCESSION   DAELXX010000047 DAELXX010000000
VERSION     DAELXX010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27696
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.047"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            207..551
                     /locus_tag="KPH29_001906"
     CDS             207..551
                     /locus_tag="KPH29_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBG1467504.1"
                     /translation="MNNKEKGDFGEEIAVNYLLSKGAKILEKNYRLRIGEVDIIANIG
                     DEVVFVEVKSRSNIKYGYPCESVDFKKRKKIIGVAKYYIIKNNLNNTPIRFDIIEVYL
                     LEKRINHIMNAF"
     gene            582..2183
                     /locus_tag="KPH29_001907"
     CDS             582..2183
                     /locus_tag="KPH29_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HBG1467505.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLLVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVSIMSFLDDKSRKENGELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISIEEMIEVSKV
                     YSILGMINESKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFNKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            2202..3302
                     /gene="dprA"
                     /locus_tag="KPH29_001908"
     CDS             2202..3302
                     /gene="dprA"
                     /locus_tag="KPH29_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBG1467506.1"
                     /translation="MEKRDTYLWLKSIGGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGYAYLENIKELLYKKAIKYICKYDKEYPENLKNIYNAPKLLF
                     YKGDIGLVNNNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            3335..5422
                     /gene="topA"
                     /locus_tag="KPH29_001909"
     CDS             3335..5422
                     /gene="topA"
                     /locus_tag="KPH29_001909"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBG1467507.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNTGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            5587..6372
                     /gene="codY"
                     /locus_tag="KPH29_001910"
     CDS             5587..6372
                     /gene="codY"
                     /locus_tag="KPH29_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBG1467508.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            6479..7744
                     /locus_tag="KPH29_001911"
     CDS             6479..7744
                     /locus_tag="KPH29_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBG1467509.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDIEKGL
                     KRAVEVLNEDSYMDIECNNNAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDAHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            7775..8545
                     /locus_tag="KPH29_001912"
     CDS             7775..8545
                     /locus_tag="KPH29_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG1467510.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMMFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDTLAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            8654..9100
                     /locus_tag="KPH29_001913"
     CDS             8654..9100
                     /locus_tag="KPH29_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBG1467511.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            9118..10311
                     /gene="nifS"
                     /locus_tag="KPH29_001914"
     CDS             9118..10311
                     /gene="nifS"
                     /locus_tag="KPH29_001914"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HBG1467512.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGRVDLRQLEDSIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLENRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            10365..10805
                     /gene="nifU"
                     /locus_tag="KPH29_001915"
     CDS             10365..10805
                     /gene="nifU"
                     /locus_tag="KPH29_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HBG1467513.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            11094..12173
                     /gene="mnmA"
                     /locus_tag="KPH29_001916"
     CDS             11094..12173
                     /gene="mnmA"
                     /locus_tag="KPH29_001916"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBG1467514.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDVVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     misc_binding    12316..12554
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12622..15261
                     /gene="alaS"
                     /locus_tag="KPH29_001917"
     CDS             12622..15261
                     /gene="alaS"
                     /locus_tag="KPH29_001917"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBG1467515.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYYLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     gene            15286..15543
                     /locus_tag="KPH29_001918"
     CDS             15286..15543
                     /locus_tag="KPH29_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBG1467516.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            15553..17724
                     /locus_tag="KPH29_001919"
     CDS             15553..17724
                     /locus_tag="KPH29_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467517.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSYASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYGTMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNVGYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIININMIIFEKNN"
     gene            17930..18355
                     /gene="ruvX"
                     /locus_tag="KPH29_001920"
     CDS             17930..18355
                     /gene="ruvX"
                     /locus_tag="KPH29_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBG1467518.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            18433..18720
                     /locus_tag="KPH29_001921"
     CDS             18433..18720
                     /locus_tag="KPH29_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBG1467519.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            19002..19460
                     /locus_tag="KPH29_001922"
     CDS             19002..19460
                     /locus_tag="KPH29_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBG1467520.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(19577..20314)
                     /locus_tag="KPH29_001923"
     CDS             complement(19577..20314)
                     /locus_tag="KPH29_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBG1467521.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            20531..22207
                     /locus_tag="KPH29_001924"
     CDS             20531..22207
                     /locus_tag="KPH29_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBG1467522.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            22413..22613
                     /locus_tag="KPH29_001925"
     CDS             22413..22613
                     /locus_tag="KPH29_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protein"
                     /protein_id="HBG1467523.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            22667..23830
                     /locus_tag="KPH29_001926"
     CDS             22667..23830
                     /locus_tag="KPH29_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG1467524.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLN
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(24041..24463)
                     /locus_tag="KPH29_001927"
     CDS             complement(24041..24463)
                     /locus_tag="KPH29_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HBG1467525.1"
                     /translation="MENKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(24484..25320)
                     /locus_tag="KPH29_001928"
     CDS             complement(24484..25320)
                     /locus_tag="KPH29_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG1467526.1"
                     /translation="MRQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIVLDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            25618..26214
                     /locus_tag="KPH29_001929"
     CDS             25618..26214
                     /locus_tag="KPH29_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HBG1467527.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAGYVLIYSMGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLY
                     KFESMSYIILLIALFTGIYQIILSPIYTVLLKIVYLFV"
     gene            26244..26963
                     /locus_tag="KPH29_001930"
     CDS             26244..26963
                     /locus_tag="KPH29_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBG1467528.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKDKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            26965..27504
                     /gene="scpB"
                     /locus_tag="KPH29_001931"
     CDS             26965..27504
                     /gene="scpB"
                     /locus_tag="KPH29_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBG1467529.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVATNE"
BASE COUNT        11209 a         2896 c         5010 g         8581 t
ORIGIN      
        1 taaatgtttt ttttctgttc attaaagaca ttcctccata aaaataaact ttcattataa
       61 aaactagcat aatatcataa aagaataaag tcaatagaag tatttattaa tatcaatgga
      121 aataaaatca atttcaaaaa attgaatagt taaattgtat actgacgata atttaaaagt
      181 aaaaatttaa tatattggag tagataatga ataataaaga aaaaggtgat tttggagaag
      241 aaatagcagt taattatctg ctatcaaaag gtgcaaagat actagaaaaa aattatagat
      301 taaggatagg ggaggtagat ataatagcaa atataggaga tgaagttgta tttgtggagg
      361 tgaaatcaag gtctaacata aagtatggct acccttgtga atctgtagat tttaaaaaaa
      421 gaaaaaagat aattggtgtc gcaaaatact atattataaa aaataactta aataatactc
      481 ccataagatt tgacatcata gaggtctatt tgttagaaaa aagaataaat cacataatga
      541 atgcttttta aaaaagataa tgtttttgga aaggattaaa tatgttaagt attattaatt
      601 caagcaactt agtaggaatc gacagctttt tagttaaggt tgaagtagat gtaagcaatg
      661 gaatacctag ttttaatatt gttggtcttc caggcaaaga aattaaagaa gcaagagaaa
      721 gagtaaagtc agcaattctt aatagtggat ataaatttcc aagcacgaga atagttgtaa
      781 atttatcacc agcagatata aaaaaagaag gagctttttt agatttatct ataagtatag
      841 gccttttaag agagttaata aaaaaagatg aaaattacat aagagaaagt atgtttatag
      901 gagaattatc attagatgga aaaataagaa aagtaagagg gatattgccc ataattatgg
      961 gtgcaaaaac tcagaacata aaacgtattt ttataccaat tgagaacata aaagaaagtc
     1021 tattagtaga tgaaattgat ataattccta ttaaaagttt aaaagaatgt gtcgattttt
     1081 taaacgaaga gataaaagtc gataaagtaa gtataatgtc ttttttggat gataagtcaa
     1141 gaaaagagaa cggagaatta gaaaaagata atagttatat agattgcaaa tatactaaga
     1201 ttaataatga agaaagtaaa tatgatgaag actttaaaga tgtaaaagga aattattttg
     1261 taaagaggag tgcagaaatt gcagcagctg gaaatcataa tatgtttatg attggacctc
     1321 caggctcagg aaaaactatg attgcaaaaa gagtaaggac aattttacca gatataagta
     1381 tagaagaaat gatagaagta agtaaggttt atagtatatt gggtatgata aatgaaagta
     1441 aaggaataat tgataaaaga ccttttagag cacctcatca tacaactaca aagcaatctt
     1501 taattggagg aggaatggat gcaagacctg gagaaattgc tttagcacat agaggtatct
     1561 tatttttaga tgagatagcc gaatttgata gaaaaatact tgagacttta agacaaccta
     1621 tagaagatgg atatgtaaat atatccagag taaagtacag tgcaaaatat ccttgtagag
     1681 tactgttagt tgcagctatg aatccatgtc catgtggtta ttatatgtca gagacagagt
     1741 gtagatgtag aagtaacgaa attgatagat acataaataa aatatcaggt ccattgttag
     1801 ataggtttga tatttttgta gaagttaatt ctataaaata ttcagatttt aattctttaa
     1861 aacaagaaga aagctctcaa aaaataaaga gaagagtaga aaatgctaga aaaatacaga
     1921 taaatagatt taagaaagat aatataaaaa ataatagtga aattaaagct tacaatttat
     1981 ttaaatattg taaactggaa aaagaagctt caaaaacggc tgagatgata tttaataaat
     2041 acaacctaag cagtagaagt tatacaaaac ttttaaaaat ggcaagaaca atagcagatt
     2101 tagaagaaag agacttgata aattcacagt gtattataga agctttttca tttagaaagg
     2161 cttattatag ttattttaaa tagttttttg aggtggattt tatggaaaaa agagatactt
     2221 atttatggtt aaaatcaatt ggtggaataa caacaaaaac aattgaaata atagaaaatg
     2281 aaatagtaaa tattgaagat atatttgatt tttctgaaaa agaaatatat aatttaaaaa
     2341 atataagctt gaatattaga aaaaatatag taaaatatag aggttacgct tatttagaaa
     2401 atataaaaga actactttac aaaaaagcga ttaaatatat ttgtaaatat gataaagaat
     2461 atcctgaaaa tttaaagaat atatacaacg ctccaaagtt attattctac aaaggtgaca
     2521 taggtttagt taataataat tttaatatag ctatagtagg ttcaagaaaa cctacagcct
     2581 atggaattaa ttgtgcaaaa acaataagtt gtcaactatc ccaatatgga gttaatatag
     2641 taagtggatt agctatagga atagatgcat actcgcacat aggttgtatg agtggaaaat
     2701 ctaaaactat agcagtactt ggttcaggag tagacaatcc tcttccaaaa caaaatttac
     2761 atttatcaaa taaaatatta gaaaatgggg gattattatt atcagagtat aatattaact
     2821 cgacagtagc cccgtaccat ttttctaata gaaatagaat tatcagtggt ttaagtgatg
     2881 gagtagttgt tgtagaagca gcaataaaaa gtggtgcact aatcacagta gattttgctc
     2941 ttgaacatgg aaaaaatgtt tttgcaatac ctggtaatat aaattctcaa atgagtagag
     3001 gttgtcataa aattataaaa gaaggtgcaa aattaattga aaatatagat gatattttaa
     3061 atgaatataa catatttaat attattgata aaaaaataaa tcaaaaatat gataatataa
     3121 gtctaaatgc caagtctaag caaataattg aagctataaa aagagaagga aacttgcata
     3181 tagatagtat ttgtgattat actggtatag aaataaagta tgtaaattca ataatcaatg
     3241 aattagtact aaatgagtta gtagtagaaa tgaataataa aacgtacagt ttaaatgtat
     3301 aaattaagat taggtagtgg gggtgtatgg ttttatggcc aaaacattag tcatcgtaga
     3361 atcgcctgca aaagctaaaa ctatagaaaa atttttaggg aaaagtcatt acacagttaa
     3421 agcatcagtt ggtcatgtca gagatttacc taaaagtaaa ttaggtgtag atatagaaaa
     3481 taattttgaa cctcaatata taaatataag aggaaaaggc gatgtaataa aagaattaaa
     3541 aaaagaagct aaaaaatcta aacaagtata tctagcaact gaccctgata gagaaggtga
     3601 agctatatca tggcatttag ctcatatact aaatttagat gaatctgagg attgtagaat
     3661 agagtttaat gagataacta aggatgcaat taaaaaagca attaaacatc ctagaaatat
     3721 tgatataaat ttggttgatg cacaacaggc aagaagagtg ctagacagac ttttaggtta
     3781 tcaaataagt cctatattat ggcaaaaggt tagaaaaggt cttagtgcag gtagagtgca
     3841 atctgttaca acaaagttaa tttgtgatag agaaaaagaa ataaaggcat ttattccaaa
     3901 agaatactgg acaatagatg ttgaggctaa gacagtaaaa gacaaagata taactttgaa
     3961 attttatggt aaaaatgatg aaaagattga attagaaaat gaagaagctg taaatgaaat
     4021 actaaaagaa atggaaggta aagatttaaa ggttgagaag atagaatcta aatcaagacg
     4081 taaatcagct ccaaaaccat ttacaacaag tatgcttcaa caagaaggag caaataagtt
     4141 attttttact actaaaaaaa caatgatgat agcacaagaa ttatatgaag gtatcgacat
     4201 agaaggtgaa ggtactgttg gtcttatatc atatataaga actgactcta aaagaatatc
     4261 tgatgaagca aaagaaaaag ctaagggtta tatcgtaaac aatttaggag aaaactacta
     4321 taaaaacact ggtgataaaa aagaaaaaaa ggatactaaa aaggttcaag atgcccatga
     4381 ggcaataaga ccaacctcag ttgatagaac tccagagagt ataaaagcct cattgagcaa
     4441 agaccagtac aagttataca atttaatttg gagaagattt gtagctagtc aaatggaaga
     4501 ttcagtgttt gatatactaa atgtagaatg taaaataggc gatattgtat ttaaggcaac
     4561 tggttcaaaa atgaaatttg atggttatac caaggtatat aatttttcag aaagagaaga
     4621 caaaatttta ccaagtatag aaaaaggaga tatattaaat atagaaaata tattgccaga
     4681 ccaacatttt acacaaccac cagcaagata tacagaagct agtttagtaa aaacactaga
     4741 agaactaggt ataggtagac caagtacgta tgctccaaca atagctacta tactgaatag
     4801 agagtatgtt gaaaagcaag gaacaagtct atgtccaaca gaacttggaa taatagttac
     4861 tgatattttg gaaaataatt tccataaatt tatagatgta gattttactg ctgatatgga
     4921 aagtaaactt gatgttatag aagaaggcaa tacagattgg aaaaagattg tatcagaatc
     4981 atatgcacca cttaaagagg ctattgaaga agctcttgaa aatatagaaa aagtaaatat
     5041 ggatgaagaa actgatgaga tttgcgagaa ctgtgggtct aatatggtta tcaaatatgg
     5101 tagatttgga aagtttatgg cttgtaaaaa ctatcccgat tgtaaaacaa ctagaccaat
     5161 tatgaataaa attggtgtta aatgtccaaa atgtgaagaa ggagatatta tacttagaaa
     5221 atctaaaaga ggtaaagctt tttatgggtg ttctaattat ccaaactgtg actttgtagc
     5281 ttggaataag ccaacaggag agttttgtga gaagtgtggt tcatatatgg ttgaaaaagt
     5341 gactaaatct gaaacaaaaa cagtgtgttc taataaagaa tgtaataaag agacaaaaga
     5401 gcataatgac ggaaaagagt gaaaattgaa aaaaatcaca taattctctt gaaaaaggtt
     5461 ccaatctatg ataatctttc aagtgtaaac aactaataga aaaataaatt ttcattaaat
     5521 gtgaaaataa tattagaaaa tataaaaggt tattaaaagg cctaaatttc atgaggagat
     5581 gattaaatgg caagtgaagt gttacaaaaa acaaggaaaa taaataaaac attacaaaca
     5641 agtggtggaa gcagtgtctc ttttgattta ctggccggag cattgggcga cgttttaagt
     5701 tctaatgttt atgtagtaag tgcaaaaggt aaagtactag gtcttcattt aaatgatgtt
     5761 caagacagtt cagttataga agatgagtat actaagcaaa agaaattttc agatgaatat
     5821 actcaaaatg tgttaaaaat tgatgaaaca ttagaaaatt taaatggtga gaagatatta
     5881 gaaatctttc ctgaagaaca tggaagatta caaaaatata ctacagtagt tccaatatta
     5941 ggaagcggtc aaagattagg aacattggta ctttcaagat attcaaattc attcaatgat
     6001 gatgatttag taatagctga atacagtgca actgttgttg gtcttgaaat attaagagca
     6061 ataggtgaag aattagaaga agaaatgaga aagaaagctg tagttcaaat ggcaataggc
     6121 actctgtcct actccgagct tgaagcagtt gaacatattt ttgctgaatt ggatggaaaa
     6181 gaaggtctac ttgtagcaag taagatagct gatagagttg gtataactag gtctgtaata
     6241 gtaaatgcac ttagaaaatt tgagagtgca ggtgtgatag aatcaagatc attaggtatg
     6301 aaaggtactc atataagaat acttaatgac aaacttacag atgaattaaa aaaattaaaa
     6361 aacaatcaat aatctatatt ttataggttt agattagaaa aggtatctat ttcaaataga
     6421 tacctttttt agacgaatta ttaaggtaat gataagtata ttaaaaagga gtgagaaaat
     6481 gcctttagca gataaaataa gaccaaaaac aatgaatgat gtaataggtc aaagtcatat
     6541 aataggtaat ggaaaaatat tgtctaagat attacaatct aattttttac caaatatgat
     6601 attttttggt cctcctggtg ttggtaaaac tacagtagcg gaaataatag cagaaagaag
     6661 caataaaagc ttctataaaa taaatgctac taattcatct ttagaagata taaaaaaggt
     6721 gatatcagag cttggaagta taaataatgt aaatggagtc ctattatata tagatgaaat
     6781 acaaagtttt aataaaaaac agcaacaatc aatattagaa tttatggaaa atgggagtat
     6841 aactttgatt gctagcacta cagaaaatcc atatcattat gtatataaag ctcttttaag
     6901 taggtctact gtatttgaat ttaaaccttt agaaaaactt gatatagaaa aaggactgaa
     6961 aagagctgtt gaagtattaa atgaagatag ctatatggat attgagtgca ataataatgc
     7021 tattgaagat atagcaattc tttctgatgg agatatgaga agagccttaa atattttgga
     7081 agtggtagtc tattcaacta aaccaaataa ggataatata acttatatag attcagatgt
     7141 aataagggca tctacattta ataaaattat aaactatgac aaaaatggtg atagtcatta
     7201 tgatatttta agtgcatttc aaaaatctat aagaggaagt gacccacagg catctataca
     7261 ctatctagca agacttataa aaggtgggga tttgataagt atatgtagga gacttttagt
     7321 aatagctagt gaagatatag gtttagctta tccaaatgct attgtaattg taaaggcatg
     7381 tgtagactca gctatgcaac ttggatttcc agaagcgaaa ataccattag cagaagctac
     7441 tattcttttg gctacatctc caaagtctaa ttctgcatgt atagcaataa tgaaagctat
     7501 ggatgaactg gataaagagt ttgttaagga tataccaaat agtataaagg atgctcacta
     7561 ttctggttct aaagaaatag gtaggggttg tggatacaaa tatcctcata attttaaaaa
     7621 tcattatgta aagcaacagt atttaccaga tagcataaaa gatagtattt attacatacc
     7681 acaagaaaat aaaactgaga aaaatataaa aaaatattta gaatatctaa atagtgattt
     7741 ttagactgaa ttttatgtat cgggggtaga tattatgatt ataaaactta actcaactta
     7801 tcacagtaga gttatgaaat acttaaaaaa agaacctgag tacaatttgt ttataatagg
     7861 ggatattgaa agatatggat atggaaataa ttttttaaat atatgggcag acgtgggtga
     7921 acatggtgaa ataaaggcaa tattacttaa atattttgaa tttatgatgt tttattctga
     7981 tggagagtat gatgttgaag gtttttataa tctacttcgt aatactaatt acgaagaaat
     8041 atctgggaaa atatgtgctg tagatacttt agcaaagaga ttaggactta ataatttaaa
     8101 ggtagttgat ttttgcaaac ttcaaactaa aaagtttttg atagataata attgtaatgc
     8161 aaaggttaag agaattaggc ttggaaatct aaaaaagaca gtgaaacttt atgacttaat
     8221 agatgaattt cacagtacaa ctttagagaa tttaaaaaat ggactaagga caggtagagg
     8281 atattgtata gagattaaca aacaggtggt gtcaatggca aagagtactt cagaaaatag
     8341 gactcatgca atgataattg gagtaggaac acatcctaaa tatagagcta aaggacttgc
     8401 tacaaaatgc ctaattaaac tatgttcaga gttactgaga gaaaacaaaa ttccttgttt
     8461 gttttatgat aatgaagagg ctggaaagat atataaaaaa ctaggatttg aaaacattgg
     8521 aaaatgggga atttattcaa aataaaaaat taaaattaat cttgacaaaa ttagtagggt
     8581 ataataaaat tatcttgaaa gtaattccta ctaaaacagt atgaattaga tttctatata
     8641 gaggtgataa tatatgaagt tgtctactaa aggtagatat ggactaaaag caatgtttga
     8701 attagcatta aatcaagaca atggaccagt atcattaaaa tttattgcta agaagcaaaa
     8761 aatatcagat cagtacttag aacaaatatt ttcatcttta aaaaaatcgg gtctagtaaa
     8821 aagtgtgaga ggtgctcaag gaggttattt gctttcaaag aatgctgaag acataacagt
     8881 aggagatata cttgtagtat tagaaggacc agttgcatta tctgactgtg tactagatga
     8941 agatgtatgt gaaaactcaa acatgtgtgt aacaaaaata gtttgggaaa agatgaaaaa
     9001 gggtatagaa gatgttatag attcaattac tcttaaagat atgataaatg actataacaa
     9061 aaataaatta gaaaatgata taacaaatat taaaaaatag atttggagat gaatgtaatg
     9121 gaaaaaagaa gattgtatat ggattattct gcaacaacac ctattaaaaa agaagtattg
     9181 gatgcaatga tgccatacct aacagattat tttggtaatg catctagttt tcacacattc
     9241 ggaagagaag caaaagatgc tttggataaa gcaagagaac aagtagcagc ccttataaat
     9301 gctgaaccta gtgaaatata ttttacagca ggtggttcag aaagtgataa ctggacacta
     9361 gagggtgtag cttatgctaa taaaaacaaa ggtaatcata taataacttc aaaaatagag
     9421 caccatgcaa tacttcatac atgtgaatac ttagcaaaac atcatggatt tgaaataact
     9481 tatttagatg ttgatagcga aggaagagta gacttaagac aactagaaga ttcaataaaa
     9541 gatactacta tacttataag cataatgttt gcaaataatg aaataggaac tatacagcct
     9601 attaaagaaa tatccgaaat agctaaaaaa cataagatat tattccacac tgatgcagta
     9661 caagctacag gtaatattcc agtagatgtt aaagaattag gaatagattt gatgagtatg
     9721 tcttcacata aaatatatgg accaaagggt gtaggagctt tatacataag aaaaggtgta
     9781 agacttcata actttgtcca tggtggagca caagaaaaaa gcaaaagagc tggtacagaa
     9841 aatatacctg caatagttgg atatggaaaa gcagcagaat tagctaagga aaatatgcaa
     9901 aatcatgtag aaactttaac tcgtcttaga aataagttaa tagatggtgt gttagaaaga
     9961 ataccttaca caagagtaaa tggtagctta gaaaatagat tgccaggaaa tgcaaacttt
    10021 gcattccaat ttatagaagg tgaaggaata cttttattat tagatatgtt aggaattgct
    10081 ggttcaagtg gttctgcatg tacttctggt tcattagacc cttcacatgt acttcttgca
    10141 ataggtttac cacatgaaat agcacatggt tcattaagac ttacagttgg agattttaca
    10201 acagatgatg atatagatta tatactagag aacttaccaa aagtaataga aagacttaga
    10261 agcatgtcac cactttatga tgatgcgaag aaacaaggtt tagtaaaata gacagaaaga
    10321 taaagcataa aaaatagaca taaattgagt aggaggaaaa gataatgcaa tatagtgata
    10381 aagttatgga acattttatg aatccaagaa atatgggaga aattgataat gcaagtggtg
    10441 taggtgaagt tggaaaccct acatgtggag atataatgaa gatattttta gacatagatg
    10501 gagatgtaat aaaggatgtt aagtttaaaa catttggctg tggttcagct atagcaagtt
    10561 cttctatggc aactgaaatg ataaaaggaa aaactataaa agatgcatta gaacttacaa
    10621 ataaagctgt tgcagaagct ttggatggtt taccaccagt aaagatgcat tgttcagttt
    10681 tagctgaaca agctgtaaaa gcagctctta tagattatgc tcaaaagaac aatatccata
    10741 taccagaatt ggatggatat gttatagatg atgctcatga ccatgatgtt gaagaagaag
    10801 aataaaaata agtgattgtg ttatgagaag tttacaatga ttaaaaagaa gtactaatga
    10861 tataattagg acataaaaaa tagctgtttt taaaatggct attttttatt gcctaaatta
    10921 agtattttaa cttgatttag aggttaatta gttaacaaaa taatccataa gattcaaata
    10981 ataaagatga ttattttttt agaaaataga acaagctata ttttaaataa gtacaatact
    11041 tgctctatat ggtataataa taagtaatgc aaaagatgag gtgaaatttg tttatgaata
    11101 aaaaagtaat gataggtatg agtggaggag ttgatagctc tgtagctgca tatcttttaa
    11161 aacaacaagg atatgatgtc attggtgtta ctatgaagtt gtggcaagat gatgatgttg
    11221 ttgaaattga aggtggttgt tgctctttat ctgctgttga agatgctaga agggtagcaa
    11281 ataaaatagg tataccattt tatgttttaa attttagaga agtgtttaaa gaaaaagtaa
    11341 tagattattt tatagatgaa tatttggaag gaaaaactcc taatccctgt atagcttgta
    11401 ataaacatat aaaatttgat gatttttata agaaggctag acaaataggt tgtgattatg
    11461 tagctacagg tcactatgca aagatagaaa aggatgaaag tacaggaaga tatttgctaa
    11521 aaaaatctgt tacagataaa aaagaccaaa cttatgcact ttataatctt acacaagagc
    11581 aactagagca tactttattg ccaataggtg actatgaaaa agatagagta agagaaattg
    11641 ctaaggaaat ggggatggca gtacacaata agcctgatag tcaagaaatt tgttttgtta
    11701 aagataatga ctatgcaaat tatgtaaaaa aacattctaa aaagcgtatt gaagaaggtt
    11761 tttttgtaga tactaaaggc aatatacttg gaaaacataa gggtatatta tattatacta
    11821 taggccaaag aaaagggctt ggaattactt ttggaaaacc aatgtttgta atagacataa
    11881 atccaataaa taataccata gttcttggag acaatgaaga tttatttaaa aaggaactta
    11941 ttgctaaaga tgttaacttt atatcaatag atacgttaga agaaccctta agagttcaag
    12001 ccaaaataag atattcagca aaaccatcac cagctactat tcatagagta ggagaagata
    12061 ctattaaaat agtttttgat gaagctcaaa gagctattac taaaggtcaa tctgtagtaa
    12121 tgtatgatgg agatattgta gtaggtggag gtataatcga gaaaagttta taaaaacatc
    12181 taaaaaatta aaaaaataag caataaagta tattaagagt aaataaagtt tataaaataa
    12241 atttattgta tattaaaata tagtattgat taattttgta aaatttagta atataattag
    12301 tatattaatt aaaaaacaaa tacagtgata aggaatagta aaatatttga tgtcttacag
    12361 agagagggaa atgctggaag cccttagaat atgatatttg aagcagcctt ttagtagcaa
    12421 tcttgaacaa tttatctttt ataaagaaga tttaagtaga gattgacggt agttacgtta
    12481 tagctaaggt tttccgaaga tatcatactt tgtatgataa ttagggtggt accgcgattg
    12541 tcctcgcccc tataaattta tttataggga cgaggttttt tatttaaacg aaaaaataaa
    12601 atttattagg aggaacatga tatggaaaag atgggattaa atgaaataag aagtaagttt
    12661 ttagaatttt ttgagtcaaa aggtcattat gtagcaaata gttattattt agtaccaaac
    12721 aatgataaga gtttgttact tataaactca ggtatggcac ctttgaaaaa ctacttttca
    12781 ggtgtagaag tacctccaag tgtgagaatg tgtacatctc aaaaatgtat aagaacagga
    12841 gatatagaaa atgtaggtat aactgcaaga catgctacat tctttgaaat gatgggtaac
    12901 ttctcatttg gagattactt taaaagagag tcaatcaaat ggggatggga atttgttaca
    12961 gaatggctaa atataccaga agataagata tgggtgactg tatatgaaga agatgatgat
    13021 tcttatgata tatgggcaaa agaaatgaat ttcccagaag aaagaatggt tagacttgga
    13081 aaagatgata atttctggga aataggtaca ggtccttgtg gtccttgttc agaaatatat
    13141 tttgatagag gagaagaata tggatgtgac aatccagact gtaaaccagg ctgtgattgt
    13201 gataggtatt tagaattttg gaaccatgta tttactcaat ttgatagaga tgaagaagga
    13261 aattatagtc tattagaaaa caagaatata gatacaggta tgggtcttga aagaatggga
    13321 tgtattatgc aaggcgtaga cactatcttt gaagttgata caattaaatc tatactagaa
    13381 gcagttgaga agttaacagg agtaaaatat ggagagaatc caaaaaatga tatatcaata
    13441 agaataataa ctgaccacat aagagctgta acattcttag ttagtgatgg agtgcttcca
    13501 tcaaatgaag gaagaggata tgttcttaga agactcctta gacgtgctgc tcgtcatggt
    13561 aagttattag gagtaaaaga attattttta cagaaactaa tagatgaagt tataaaggta
    13621 aatgataaag catatccagt tttagttgaa aaagaaagtt atattaaaaa agtagttgga
    13681 atagaagaag aaaaattcaa tgaaactata gaccaaggaa cagaaatatt gaactcttat
    13741 atagaagtat taaaaaatga aggaaagact gtcctaagtg gtcaagaagc ttttaaacta
    13801 tatgatactt atggcttccc tatagactta actaaagaaa tattagaaga agaacatcta
    13861 tctgtagatg aagaagcttt taatgaagaa atggaaaagc aaaaagaaag agctagaaat
    13921 gctagaggaa atatggatgg agaaagttgg aaggaagacc ctctatcaaa attagaatct
    13981 acagttgaca gtacatttaa tgggtatagt gaaatttatg gtgaaggaac tattgaagct
    14041 atagttaaag atgatgagct agttcaaagt gcagaagaag gcgataaggt atctatagta
    14101 ctagataaca ctactttcta tcctgaaggt ggaggacaag ttggagattg tggattgata
    14161 actaatgaaa acttagtttt agaagtatta aatactaaaa aaggtgctaa taatagtata
    14221 aaacatatag gtataataaa atcaggaaga ataagtaatg gggacaaagt aaagacactc
    14281 gtagatagag aaactagaat gtcagctgca agaaatcaca gtgctacaca tttacttcat
    14341 aaggcattaa gagaagtatt aggagaacat gttaatcaag ctggttcttt agtaactcca
    14401 gaaagactta gatttgatat tactcacttt gaagcgatat caaatgaaga attaaaagta
    14461 atagaagaaa aagtaaataa tgttatttta tcatctttag acataaaatg tgatattatg
    14521 aatataaagg aagctaaaga aaaaggagct acagcactat ttggtgaaaa atatggtgat
    14581 gaggtaagag tagtttctat gggagattat tctacagaac tttgtggagg aactcacttg
    14641 acaaatactt ctcaagtagg aatgttcaaa atattatctg aaggtggagt agcagcaggt
    14701 gtaagaagaa tagaagcaat aacaggaaaa gctgtttatg aatacctaaa agaaagagat
    14761 ggaattatct ctgaagtttg tgtaaactta aaatctaaag aagataattt aattcaaaga
    14821 ataagttctt tattagaaga aaataaaaac ttatctaaag aattgcatga tatgaaagct
    14881 aagatgagtt tacaatcagt tgattctatt tttgattcta aagttgaagt aaatggagtt
    14941 aatttgataa ctaataaatt tgaaggtatg gatatggata cactaagaga aacagcagat
    15001 aatctaagag ataagcttgg ttctggtgta gtagtacttg caaatgtagt agatgataaa
    15061 gttaactttg tagttactgc cactaaagat gtattagata agggaataca ttcaggtaat
    15121 attgttagag aagttgcaaa aatagctgga ggaaaaggtg gaggaagacc taatatggca
    15181 caagcaggag ctagtgatgt atctaaggtt gaccaagctt taagctatgc aagtgaggtt
    15241 attaagacac aagttaaata acattcactc aaggaggttt ttagtttgga aaaagattta
    15301 gactacacta tgaaatttga aggaatacca gaagatagga tgagtgtagg agatactatt
    15361 gattttgtat ataaagcatt agttgaaaaa ggatataacc ctataaatca aattataggg
    15421 tatctccttt ctggtgattc tagctatata actagtcata aaaatgcaag agctataatt
    15481 aaaaaatttg aaagagatga gatacttgaa gaagtaatca cgcactatct gaatagaaag
    15541 taggtcaaat aaatgagaaa aaagattatg tcgttgtttg caatagtcat tatgattttt
    15601 gtatcaactg ctagctatgg ttttgctgaa gaaaaaggca aggtcatatt tattgatatg
    15661 aataggacca atcttggaag tatgatgaat attccaatct tgaaaaatga gatagaaaag
    15721 agaggctatg tagctctcat gaatataaga ggggatcaag gtactgatga tagaaggtct
    15781 tatgcaagta tgggtgcagg aacaagagct agtgtaacta ctgaagacta tatcaatttt
    15841 caaaaagtag accaagatac tgctaaagct tttgaagtcg caacaggtca aaaagctaaa
    15901 gaaataaatg atttaggcat taatagatct ataaatgaaa atcttgagta tggagaatat
    15961 ggagcaaatt tagggtcatt aggaaaaact ttatcagaaa atggtttaaa gacatctgta
    16021 attggaaatg ctgatataat tgaaaataat caattagtta aaaatagaaa cttatgttta
    16081 gtagctatgg accaatatgg aaggattgat tcaggtaata ttgataatat taataagaaa
    16141 gattctaaaa tgccatttgg aatatctaca gactacgata aattactaga tgaaactaaa
    16201 aaatcctata aaaatagtga tgctatattt gtagaacttg gagatacata tagattagat
    16261 atgtatagat taaatctaaa tgataatact tatggtacaa tgaagaaaaa tattgtaaaa
    16321 aatataaata aatatcttga acaagtattt tctatggttg gagaaaatga cactgtttat
    16381 atagcaagtg catttccaag tgacctagat tataagaata agagaagact ttctccaata
    16441 ataaaattta gtggagaagg gaaaggtcta ctggaatctg ccacaacaag aaaggctgga
    16501 attgttgcca atttagatgt tggagtagat atactaggta catttggtct tgaaaataaa
    16561 gagatggtag gtagaagtta tactttagta aataaagagg acaatgttgg ctatctaaaa
    16621 tcactttatg aaaggatagt gtctatatct tcaataaggt caactattat aaatggcttt
    16681 gtaggcgtaa tatctgcttc ttgggtaatt gccatgtttg cgattttctt tagaaattat
    16741 atgccacata aagagcgtgt attcaaagta cttaaggagt ttataaaatt aggcataata
    16801 atgccactat catttatgtt agctcctttg atgaatttta aaactccaat ttcaatatct
    16861 ttgggaatat ttgcgactac gctaatatta tacttgttag gtagaaaatt atttaaggac
    16921 aatgatataa agcacatggg attttttgca acacttacta ttttagtcat agttatagat
    16981 tctgtatttg gaacatactt aatgcaaaat aacataatga gttatgatgc tgtcatagga
    17041 gctaggtact atggtgtagg aaatgaatat gaaggtgtaa ctatagcaag tgcagtattt
    17101 ggcttagcag tattattaca ttataagaag gtatctaaat tacttgttgt aattttttcg
    17161 ttaataatac ttataactag tgcatatcct tcaatgggtg caaatgttgg tggagctata
    17221 tcagaatgtg tagcatattt gttgtttata atgcttatat atgacattaa aatagatttt
    17281 aagaaagctg tattattagc tgtttcagct gtagttgtcg tttttgcatt tgcagcttta
    17341 gatatacttt caggtagtga atctcattta ggtatgtttg tacagagaat tttcctaaat
    17401 ggaccaggag agatattaca aacatttgga agaaaaattc aaatgaatat gcaactagca
    17461 cagacaagtg tatgggtaaa tattttactt gtaggaattg gagtaattgc agtacttata
    17521 tttaaaccat ctagacactt tagaaagata atgaataatt ttccaatctt gtttaaaggg
    17581 tttattgcat ctatggttgg ttgtataatt actcttttag ttaatgattc tggaatagtt
    17641 gctgcatcta cagcatctat atatatactt ataccactaa taataataaa tataaatatg
    17701 ataatatttg aaaaaaacaa ctaaaaaatt ttttactgtt catataggat ttaaataaaa
    17761 aatgcaatat gattattaat gtaaactgga tgcttacata aaaaatcata ttgcaatttt
    17821 tatgattgat tatgcaaaat tctatgaagc aggaagcctg tgaccttagt tatgagaggt
    17881 tcagaatatg tatttaatct atagaatata aatagataaa ggaggaacta tgttagatgg
    17941 aagaataatg ggtcttgatg ttggtgataa aactattgga gttgctgtga gtgatttgat
    18001 gggattaact gcacaaggtg taaaaacaat aaaaagagtt ggcaagaaaa aagatattga
    18061 agagttaaaa gcaataataa aagaaaaaca agtaaataaa attgtatcag gattaccaaa
    18121 aaatatgaat ggaactttag gtccacaagg agaaaaagta atcaagtttt gtgaattggt
    18181 aaaagcagaa acaggtattg atgtagagtt ttgggatgag aggctttcta cagtagcggc
    18241 agagaggtct ttattagaag cagatgttag tagacaaaaa agaaaaaagg ttatagatat
    18301 gttggcagca gtaataattt tgcaaggata tttagatttt aaaataaatt cataaaagaa
    18361 ctataaaatt gaatacttat gatatataat ataaataata aaaataaaaa taaatttgag
    18421 gtgaatgaca ttatggaaga aaatataata aatttaatag acgaaaatgg cgttgaaagc
    18481 caatttgaaa taatattaac attagaagca gaaggaaaag agtatgcaat attaatgcct
    18541 ttagatgatg aagaagctga agaagcttta atctttagaa ttgatgagga tgaagaaggt
    18601 gaaatattaa tacctttgga aagtgatgaa gagtatgaaa ctgttgtagc tgtgtatact
    18661 gctataatgg aagaagaagg tttaaattac gacgaagatg aatcaaatgg acttaactag
    18721 ataagtaatt aagtttgtat ataaaaatca gatatttatt atatttgctt taattaaacc
    18781 ttattaattg taaaagcatt tatagtatat atctgttttt ttgtttttta ttaaaaatac
    18841 caaaaaattt atgaattttg gtgaataata tatcttttat gggtatatat ttatattatg
    18901 agaataaatt aaatttattt aaatgtcttg acaaaaaatg aattatcaat tacaatatta
    18961 tttaataaag attatcaatt gaaaatagta agggtgatgt tatggcaaat acaatggatt
    19021 tattaaaaga caagttaaaa gaaactggtt ttaagattac gccacaaaga agagcaatag
    19081 ttgaaatatt gcttaaacat gaccattcac atctaagtag tgaggaaatc tatgatttag
    19141 ttagggttga ctgtcctgaa ataggcttag ctactgtata tagaacaatg cagctattgg
    19201 atgaaatagg attaatatca aaactaaact tagacgatgg atgtataaga tatgagatta
    19261 gtctacataa agaagactgt cataatcatc atcatcttat ttgtaaaaat tgtggaaaga
    19321 taatggaagc taaagaagac cttttagaca atatagaaaa agagatacaa tcattgtaca
    19381 aatttaaaat acttgatcat gatgttaaat tttatggatt atgtgacgag tgtaatggag
    19441 taagcgattc agaagaataa acttctattg cttaatgtcg atggaagaat agtatagaac
    19501 ttttatattc tatgctattc ttccattttt tattataaac catctacaaa atgcaaatat
    19561 acagttttca agatgcttat agttttaata ttgaaaatat aattaatcct ataagtacaa
    19621 aaatgaatga tttttctatg aaattactaa atggtcctcc aacagttaaa aaacctaaag
    19681 aaattttctt ttcgcttagc ggataaaaaa ttggtattcc ttgctttgta aataaatctc
    19741 ctaaaaacat atgacttgca taaccaatcg taccataaaa agataaatta ttaatattag
    19801 tgattaattc tatctgtttt agtaaaaaat atactattat agttgcaaat atgctatgag
    19861 aaaaacttct gtgctttaac cagggaaaaa ttgacaacat tagacaaaaa attgtaaaat
    19921 atatttcaac ttctatgatg tataagcata tagctaatag tataaatatt aatgatagaa
    19981 aaacttttct tagaaatgtg tgggttagtt ttgcttcaat aattataatt gcaacaaatg
    20041 taattattgc gcttaaataa aaattatcat ttatatttat tgaaataaag aaaataataa
    20101 tattaacagc atatataaaa attctatata tgtatttgga tacattgttt tttagaatga
    20161 aatttgagac catagaatta gatttatcca aatcgggtag tacagcaaaa gtagctgaaa
    20221 ctaaaatatc aactaaagat ataggaattt taaatattaa agatgtttgg atagctgtta
    20281 agattcctat tgcacaatga gttttaccac gcataaatcc tcctaaatta tccgatatta
    20341 aatcatataa tatttagagg atatcaaaat aaaataggtt tgtcaaaata atacgttaaa
    20401 tgtattttaa atatagctta aaatatgata cgaattatgg tacaataaga ataactggta
    20461 gcgagtgcgt gatttgatat tatttatgat tgtagaacgt gttaaaaaat taaatagaca
    20521 gaaggagatg atgcaattgt tcaaaaagaa caccaacaag ataaaggtaa tggccttggg
    20581 tggacttaac gaagttggaa aaaatatgac tgttgttgag tataaagatg aaataattgt
    20641 tatagatgct ggacttagtt ttccagagga tgaaatgtta ggagtagata tagttatacc
    20701 agatataact tatttagtaa aaaataggga taaaataaag ggtatattta ttacacatgg
    20761 acatgaagac catataggag ctcttccata tatattaaag aaaataaatg tacctgtata
    20821 tggggcaaga cttagtatag gtcttataca agtaaaactt aaagaacata aaatgaataa
    20881 tgttaagtta aatgttatag gtcctagaca agttataaag cttgataata tggaagtaga
    20941 gtttttgaaa aataaccata gtataccaga tgcttactct attgcaatac acacagacca
    21001 aggtataata taccacacag gagattttaa gattgacttg actcctattg atggagatgt
    21061 tatggatatg catagaatct gtgagttgag caaaaaagga gttcttttaa tgctggcaga
    21121 tagtactaat gcagaaaaac caggatttac tatgtctgaa aagactgttg gggtaggtct
    21181 tgacgaatta tttgcaaaag gaaatggcag aagaattata gttgctacct ttgcatccaa
    21241 catacacaga ttacaacaaa taataaatac tgctgaaaag tttaatagaa aagttgctat
    21301 atcaggtcgc tcaatggtca atgtagtcgg agttgcaaaa gaattaggat acctagatat
    21361 ttctgatgac atgcttatag atttaaatga tatatgtaag tatgaagata gtgaattagt
    21421 gataataaca actggttcac aaggtgaacc tatgtcagct cttgctagaa tggctttttc
    21481 agagcacaaa aaagtagaaa taaagagtgg cgatttagtt ataatatctg ctcatccaat
    21541 accaggaaat gaaaaattaa tatcaagagt aatcaatttc ttatttgaaa aaggagcaga
    21601 agtagtatac agtgatatag ctgatataca cgtttctgga catgcctgtc aagaagaatt
    21661 aaaacttata catgcattag ttagaccaaa gttttttatg ccagctcatg gtgaatatag
    21721 aatgttaaaa agacatgctg aaatagctga acagcttggt atggataaag aaaatatttt
    21781 tgtcatgcaa acaggagatg ttttagaatt agataaaaac tctgctaagg tagcaaaccg
    21841 tatacaaaca ggaaacatat tagttgatgg tctaggagta ggcgatgtag gtaatatagt
    21901 tttaagggat agaaagcatt tatcagaaga tggtcttatg atagttgtag ttactatttc
    21961 caaggatgag ggtaaggtct tagcaggtcc agatataatt tccagaggat tcgtatatgt
    22021 aagagagtca gaagatttaa tggatggagc aaaggatata attaagaatg ttctaaatga
    22081 atgtgaagag aaaaatatta aagaatgggc ttatttaaag aataatatta aagaaaacct
    22141 aaaagaatat ttatatcaaa aaactaaacg aaatccaatg atacttccta tcataatgga
    22201 agtataatat aaaaaaacta taaatttgaa aattagacct cttgtaattt ttaaagtaat
    22261 atcaaaaatc caattgatat tatcttaaga attacaagag gtctttttaa tactcattta
    22321 aaaaaaatat atattacata aaataccaat tataacaatc ttttattagt aaaaactact
    22381 tatatataaa ttttactgat aggaggattc ttatggatga tgtttcaaga caaaatgcta
    22441 taaaagcttt aaaacaaaca aaaatggaaa tagcaggaga gtatggaatg aattatgaag
    22501 atgcttttga aataatagaa aatgcgtcta ataaaggtgt tttagaaggt tactttaaaa
    22561 agcttgaaaa gaaaaaaaat ttaggtcaag gtatatctag gcatttagaa taaaaaaagt
    22621 taaatgtgag aaaataagtt taaataaact ttaagaggtg acatcgatga aaaaattagc
    22681 tagtactgct ttggctatat taattgcgct tactccttta agcttttctt ttgcaaataa
    22741 taaggaaaat gctgatgcca accaactaaa tatatcttca aaatcagcaa ttttgatgga
    22801 tgttggtagt ggacaaattt tatatgaaaa ggatgctcat aaaaagcttc ctcctgctag
    22861 tgtaactaag gtaatgacta tgctacttat agtagaagct ttggattcag gcaagattaa
    22921 gcttgatgat gaagttcaag taagtgaaac tgcatctagt atgggtggta gtcaaatatt
    22981 cctagagcca ggagaaactc aaaaggttga tacattatta aaaggtatag ctgttgcttc
    23041 tgcaaatgat gcttgtgttg ctatggctga acacttagct ggaagtgttg aaggatttgt
    23101 tgatagaatg aatgctaaag caaaagagtt aaatatgaat gatactcatt ttgcaaatac
    23161 aaatggactt ccagttgcaa accactatac atctgctcat gatatagctt taatgtctag
    23221 agaactatta aaacacgaaa tgataagtaa atacttaact acatggatgg ataaggttgt
    23281 agtagggaag aaacaagtta cagtaggtct tgcaaatact aataaactta taaagcatta
    23341 tcaaggagca actggtgtta aaactggttt tactcaagaa gcaaaatact gtttatcagc
    23401 atctgcaaag agaggtaata ctcatctagt tgctgcaact ttaggagcag agacttctcc
    23461 agaaagattt aatgatgcct ctagcctttt aaactatgga tttgctaatt atgaaagtgt
    23521 taagttatgt agtaaaggtg ataatatagc aacacttaca ttggataagg ctgatgaaaa
    23581 caaagttaaa ttggttgcta aagaagattt aaatgctctt attaaaaaag gaagctctaa
    23641 agaatttgag aagaaaattg aaatagtaaa aaatcctaag atgccaatta aaaagggaac
    23701 tgttttagga aaaataaaga tatgtaaaga taaaaaagta ataggcgaag tggaattgat
    23761 aaatactaag gatataaata aggcaagcta tctacaaatg cttcaaagaa taattgataa
    23821 tatgatataa ataattatca gatgaaatat aattataata cacaaagata tgattataat
    23881 acacataaaa aataccctta tgtagttatt tgccataggg gtatttttat ttaccatata
    23941 gaagtatttt ataatcttat gatttttata gaaatataga aaagtttatg tatcttaaaa
    24001 tatcatttga tttaatatca tatatagggt tgtttaacaa ttatctaagt aaaggcatac
    24061 taccagtaag tttattcact ttctttttat tcccataaat tgctatccca aaatactgta
    24121 actcatcttc tggaacacta gcaatctttt gtgtaaattc ttcataaaga ttgcaacttt
    24181 gtgctacatc tgaaaaatct acaacagtta aatcgttaaa ttccaaagta tataattttt
    24241 tacgtaaatc ttttattatt tctttgtttc cttttaaaat aggtacagga atgtcaataa
    24301 ttcctaagtg gctattacct gttttgtcaa ttacatctgg cccaacagtt tctggggcat
    24361 gttttcctag tgtaattccc attatagctg ctgtattgga tataattccc attggtaaat
    24421 tttcgtctat taccatgaca cattttgaat ttttattttc catttttatt tctccttttt
    24481 accttattta aagttgtttg aatcatcatt tataaatata ttttgatact gctttggtgt
    24541 aagaccaata aaattcttaa aaaagtttgt aaagtgactt tggtctacaa atcctgtttg
    24601 tatcgcagca tctataggtt ctacgccttt ttctaaaagc tttttagctt tgtttacccg
    24661 tatattttct aggtatctat atggtgtaat accttttaat ttcgtaaaat tacgaagtag
    24721 agagtactta ttcatacctg ctatagtgct taattcgtca agtactatat gttcagcata
    24781 attattttct agataatcac atactgcttg aatttctata tttgtatttt ccaagttaga
    24841 ttgcaaattt ggctctgtat gttcttcaat taattgctca attaggaaga aaaaaagctc
    24901 ttccttttta aaatccaatt cttcttccat gattatatag tgtaattctt gtagtagagg
    24961 tactagctca ctgcgaaata taacaggttg attaaatttt ggtaaataat ttttacctgt
    25021 aatttcaaag acggtttttt tcattatttc tggcttaata ttgatacatc tgtaatcaag
    25081 tactttatca tctatttgtt cacaagtatg actgtcaaaa gggttaaaaa gtagcaaatc
    25141 tccagtactt gtggtatact ctttattttt acaagataaa tatctttgac ctttttctat
    25201 aaaaccaatt acataatgtt catgaaaatg gtttgggaat ttttgcatta tgcctttaaa
    25261 tctatatgct tcaattttta attccctatc aaagcatact gtgcgttctt cttgtctcac
    25321 ttaggtaacc tcctttcatg ttatacaaag tgtaacacat atttttttat acttcttgta
    25381 taatattgac taagagacaa taatgtcaat tcatgtcaat ataatattac acttacagta
    25441 ggtgtaatta ttatattttc acaatgaata tgatataata ataaaagtta taaaataaaa
    25501 agcaaatttt aattttatgc ctagaagtta aaattattat aatgtaaaat aattttgatt
    25561 ataagctatt taattgttat aataatatta aaaatttaat ttaggagatg tacattaatg
    25621 ttaagtatga atataggaga aatagtagca agtttagttg gtatagctat ggcaatatca
    25681 atacatgagt ttggccatgc ttattcagct cacttattgg gagatgatac agctaaagct
    25741 tatggtagaa tgacattaaa tcctgtaaga catgttgatc caataggtct tttggctatg
    25801 tttatcctta agatagggtg ggcaaagcca gtaccagtaa atccaaataa ttttaaaaac
    25861 tataagatag gaaacctgat agtttcactt gcaggcgttt tttgtaatgt tttgactgct
    25921 atactgtgtg taattatcaa taagtatgtg catatgtatg ctataaatac aatagctgga
    25981 tatgtgctta tatatagtat gggatttgca gcttttaact tacttccgat acctccactt
    26041 gatggatggg gagttatatc tacatttgta ccatataaat ggaatgaata tttgtataaa
    26101 tttgaaagta tgagttatat aattttatta attgctttat ttactggtat atatcaaata
    26161 atactatcac caatatatac agtgttgcta aaaatagtat atttatttgt ttaatataat
    26221 aaacagatta aagagggatt gatatgaagt ataatataca attacaagtc tatgagggac
    26281 cattagattt actttatgat ttaataacaa agcataaaat agatataaag gatatatcta
    26341 taatagatat aactaagcag tatctaaatt atttgaaaat gctagataaa atggatttag
    26401 agataactag tgaatttata actatggcat caaaactttt agagataaag tctaaatatc
    26461 tgctttataa gcaaaaagat gaagaagaag acccaagaat agaacttatg gaaaagttag
    26521 aagaatatag aaagtttaag gtagcatcac aagatataaa agaaaatata acttatgtta
    26581 atgagagatt ttatagaaat aaagaagaga taattattga tgacaatgtg gatttagaag
    26641 atatatcaat tgaagcaata aaaaatatac ttccatacat atttaaagtt aaaacatcac
    26701 agatagaaaa tgctaatgat gagaaactag acaaaatagt aaggaagaag ataatatctg
    26761 tggaagaaaa gatattgtat ataagagata ttataaaaga taagattgaa gttactttta
    26821 caaatattat aaaatcatat gaaaatgatg aaataatagc tactttttta tctatattag
    26881 agcttataaa agaaaaagaa atagtagtag tacaagacat attttttgat gatattctaa
    26941 taagaaaaag ttcggagtgt taaaatgaaa agagaagata taaagtatat tatagaatct
    27001 gttatgtttg catatgggga accaataagt ataaaagaat taaactatat aataaataaa
    27061 gaactttcat caaaagaaat agagattatg ttaaatttac ttattgagga gtacagagaa
    27121 caaaatagag gtattcaaat aatcaagcta gagaataagt accaaatgtg tacaaataaa
    27181 gactatgcag aatatataaa gaagattatt gaaccaaaga aaaagaaaag tttgagccaa
    27241 gctacattag aaactcttac tataattgcc tacaaacaac caataacaaa ggtagaaata
    27301 gaggatataa gaggagtaaa atgtgataag gtattacaaa ctttgtttga aaatgaactt
    27361 ataagagaag ctggtagatt gaataagata gggaaaccaa taatttataa aacaacagat
    27421 gaatttttaa agttgttgaa tatagaaagt cttgaagaat tgccgcctat agaaaattat
    27481 caagaagtgg caacaaatga ataaaaatat aaaatcacaa taatttagta tgtaatttta
    27541 aattttaaaa gatttaaaat gctctaagtt gttgtgtttt tttgtataaa tataaaatta
    27601 taattctctt aaaataaata tggataatta agtataaata gtaatttttt gatgataata
    27661 taaacatgga gcttaatgaa ttatttaatt ttaagt
//
LOCUS       DAELXX010000048        27694 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.048, whole genome shotgun sequence.
ACCESSION   DAELXX010000048 DAELXX010000000
VERSION     DAELXX010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27694
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.048"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            73..146
                     /locus_tag="KPH29_001932"
     tRNA            73..146
                     /locus_tag="KPH29_001932"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:105..107,aa:Gly,seq:tcc)
     gene            155..271
                     /gene="rrf"
                     /locus_tag="KPH29_001933"
     rRNA            155..271
                     /gene="rrf"
                     /locus_tag="KPH29_001933"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            545..2563
                     /locus_tag="KPH29_001934"
     CDS             545..2563
                     /locus_tag="KPH29_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG1467530.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTESYEGTINYNKDPE
                     YLNVWELQGITINSKNNPKTLNKQELEKMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPRDVIIIGDENAISKTTANQIKSTVNASQTRLKGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKNSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNDITKDNVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKDNLEAKSANKVY
                     KIGGGLTSKVMNSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSDTGTTGKPLGGIK
                     EKDYTLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLRPDLFTSIHYNAS
                     DTTGNGVEVFYKLKDKDGGTTKTVATNILNRILEKFNLKNRGAKTRTLSTDPTKDYLY
                     VLRNNDMPAVLVECAFLDNEKDMSLLNTSNKVKEMGTQIGKGIEDSLK"
     gene            3076..5088
                     /locus_tag="KPH29_001935"
     CDS             3076..5088
                     /locus_tag="KPH29_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG1467531.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTGAYEGTINYNQDPE
                     YLNVWELQGITINSKNNPKTLNGQDLEKMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTVNASQTRLNGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKDSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNGITKDSVTNNRVYGADRHETNANVIKKFY
                     TEDEIEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKDNLEAKSANKVY
                     KIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSAPGNSSGGMIEKD
                     YNLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLKPDLFTSIHYNGSTNK
                     QGHGVEVFYKLKDKNGGTTKTVATNILNRILEKFKLTNRGIKTRVLPSDSTKDYLYVL
                     RSNDMPAVLVECAFLDNENDMSLINSSAKVKEMGTQIGKGIEDSLK"
     gene            5316..7229
                     /locus_tag="KPH29_001936"
     CDS             5316..7229
                     /locus_tag="KPH29_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467532.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTDNNSRCILLLVPNNNT
                     KIRYERALRLNYSEELKITTYISFVKKELVKYWPLIIEKCDEINTKVVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIK
                     PKEENIKKKEIRIEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEENVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFSTKKDKKAVDYPYGNALVVATCIFYGYLDFIKD
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDNIDEHSQYRDILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIAESESFSESLSLLRMKKEKIFIEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSNIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTNEMEEYYKKYYLYNTVYNLLLNAKKIYAFKSEYAINGYIQESILYSI
                     LLKISHKGDKNYD"
     gene            7222..9441
                     /locus_tag="KPH29_001937"
     CDS             7222..9441
                     /locus_tag="KPH29_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBG1467533.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKGGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEIALEAWE
                     RGFFEFAGNAISELKYKGISPDKLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     EECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKYLISENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            9550..10380
                     /locus_tag="KPH29_001938"
     CDS             9550..10380
                     /locus_tag="KPH29_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBG1467534.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            10370..13837
                     /gene="addB"
                     /locus_tag="KPH29_001939"
     CDS             10370..13837
                     /gene="addB"
                     /locus_tag="KPH29_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HBG1467535.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNPTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIEN
                     LIVNFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINVIKNYDSDDYNTEEINSIWLDIYR
                     YYLKDEIYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGDNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSTICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            13837..17664
                     /gene="addA"
                     /locus_tag="KPH29_001940"
     CDS             13837..17664
                     /gene="addA"
                     /locus_tag="KPH29_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBG1467536.1"
                     /translation="MSSPKWTKEQLEVIGSRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIANISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDIDEKGNIVP
                     SDIAVGYRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKENEIEEEEK
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNETESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKNDILVEKKSDEEKESIEEILEKIDVDSPDSDY
                     YSEIEKKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            17710..18921
                     /locus_tag="KPH29_001941"
     CDS             17710..18921
                     /locus_tag="KPH29_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBG1467537.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVIATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            18908..22435
                     /locus_tag="KPH29_001942"
     CDS             18908..22435
                     /locus_tag="KPH29_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG1467538.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNIQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKNNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKIDEDSFVVSNLEKKLAILNQELEVTKNRYEKIN
                     KIKNEEVPKLSEEKIRLQQAIKLEEELVLLDRELKDLKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRS
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHNITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKLKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVINITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            22566..23819
                     /locus_tag="KPH29_001943"
     CDS             22566..23819
                     /locus_tag="KPH29_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBG1467539.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
     gene            23968..25019
                     /gene="ytvI"
                     /locus_tag="KPH29_001944"
                     /pseudo
     CDS             23968..25019
                     /gene="ytvI"
                     /locus_tag="KPH29_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
     gene            25110..26336
                     /gene="pepT"
                     /locus_tag="KPH29_001945"
     CDS             25110..26336
                     /gene="pepT"
                     /locus_tag="KPH29_001945"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBG1467540.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPRIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCREKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            26543..27595
                     /locus_tag="KPH29_001946"
     CDS             26543..27595
                     /locus_tag="KPH29_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HBG1467541.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADTISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVTEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
BASE COUNT        11739 a         2720 c         4907 g         8328 t
ORIGIN      
        1 attaggatat atatgtagtt tttagggtgc taactctaaa aaattagttt tactaattta
       61 taaaaattta atgcgggtgt agctcaatgg tagagttctg gccttccaag ccagctgtga
      121 gggttcgatc cccttcaccc gctccaaaag attatgtggt tacaatagca gagaggatac
      181 acctgttccc attccgaaca cagaagttaa gctctctagt gctgatggta cttggtggga
      241 aactgcctgg gagagtagga cgtagccacg taatcttttt ttgttgtatt aaatttaata
      301 aacctacaat tgttatatgt ttttatttgt cttttataga tttttttggc aataaatgat
      361 atagtgaaga taattaaata tattcaatat atttaatttt tttatttttt gtttttataa
      421 tttttttaga agtttatttt ttatgtaatt atttataatt ttgggtaaaa acactttgat
      481 ataatttcaa aaattaaaag gttaaaaaac aaagtagaaa tttatgttaa ataaggagat
      541 taatatgaga agaaatacaa aattattaac aacagggatt ctttcaatgg caatcgtcgc
      601 acctacaatg gcatttgcta ctgaatctaa tgctatggaa aataacgctg atttaaatat
      661 aaacttagag aaaaaaagta tcgttttagg tagcaaatca aaagtcagtg tcaagtttaa
      721 agaaaaacca gatgcagata gcattacatt aaagtataaa tgctatgata tgccattgga
      781 tacaactcta aattataatc aatcaactga gtcatatgaa ggaactatca attataataa
      841 agacccagaa tatttaaatg tttgggaact acaagggata acaataaaca gcaaaaataa
      901 tcctaaaact ttaaacaaac aagaactaga aaagatggga ttaaatttaa aagactataa
      961 tgtaacgcag gaatgtataa ttgaagatat aacttctaga aaagatgtaa ataaatattt
     1021 aagaaaaact tctgcaccta ttacagaact tacaggaagt gatagatacg aaacagcagt
     1081 taaaataagt aaagaaggct ggaaaaatgg ttcagataag gtagttataa taaatgggga
     1141 tgtaagtata gatggcatta tatcaactcc actagcaact acatataatg ctccaatact
     1201 tttggttgaa aaaaataatg tacctaatag tgtaaaatca gaattaaagc gtttaaatcc
     1261 tagagatgta attataattg gagatgagaa tgctatttct aaaactactg ctaatcaaat
     1321 taaatcgact gtaaatgcta gtcaaacacg tctaaagggc tctaatagat atgagacatc
     1381 tttattgata gcaaaggaaa tagataaaaa tcatgatgtg gaaaaagtat acataacaaa
     1441 tgctaatggc ggagaagtgg atgcacttac tatagcagca aaagcaggtc aagacaagca
     1501 accaattata ttaactgata aaaatagtat tacagacaac acatataaat ggttaaagag
     1561 tgaggattta caaaatgctt attttatagg tggacctcaa atgatatcaa caaatgttat
     1621 aaataaggta aatgatataa ctaaagataa tgttactaat aatagagtat atggagcaga
     1681 tagacacgaa acaaatgcaa acgtaataaa aaaattctat acagatgatg agttagaggc
     1741 tgttttagta gctaaatcag atgtacttgt tgatgcttta gcagcaggtc cattggctgc
     1801 gaacttaaaa tctccaatac ttataacacc aaagacgtat gtatctgcat accataaaga
     1861 taatttagaa gctaaatcag ctaataaagt atataaaata ggaggaggat tgacttctaa
     1921 ggtaatgaac tctatagcat catcattatc taaacacaat acaactccaa cagaaccagg
     1981 aaatagtggg ggtaagacag ttatgattga cccaggacat ggtggttcgg acactggaac
     2041 aacaggtaaa ccattagggg gtattaaaga aaaagactat acattaaata cttcacttgc
     2101 gacaactgaa tatttacgtt caaagggatt caatgtaata atgacaagag acacagataa
     2161 gactttatct cttggaaata gaactgcttt atctaactct ttgagaccag atttatttac
     2221 gagtatacac tataatgcat ctgatacaac tgggaatggt gtagaagtat tttataagct
     2281 taaagataaa gatggaggga ctactaaaac tgtagctaca aatatattaa atagaatctt
     2341 agagaaattt aatctaaaaa atagaggtgc aaaaacaaga acactatcaa ctgaccctac
     2401 aaaagattat ttatatgttt taagaaataa tgatatgcca gctgtacttg tagaatgtgc
     2461 atttttggat aatgagaaag atatgagttt acttaataca tcaaataaag taaaagaaat
     2521 gggtacacaa ataggtaaag gcatagaaga ttcattaaaa tagtctacaa tgagagcata
     2581 gaaaaattta aaataaatta atctgcaatt ttaaatttaa actaaagcta taagataaga
     2641 atgtaattat aatagtaata aagactgtat atacattgtg catataagtg cgaaatgagg
     2701 tatatacagt ctttttatga ttaattgtaa aaggatttat attatatgaa gaatttacat
     2761 atcttaaggc tacagttaat tgtcaagagt tggagtattc atttgtaata cacaataaaa
     2821 gcttaattac tatattttat tgtgtgtttg tgtgtttgtg tgttttttgt ggatttttat
     2881 gtcaataaat gatatagtat agataattaa atgtattaaa tatatttaat ttttttattt
     2941 tttgagtttt ataagtttat ttttatatat tacttatata ttatttatat tttgggttag
     3001 atacactttg acatattttc aaaagttaaa agattaaaaa taaagtagaa atttatgtta
     3061 agtaaggaga ttaatatgag aagaaataca aaattattaa caacagggat tctttcaatg
     3121 gcaatcgtcg cacctacaat ggcatttgct actgaatcta atgctatgga aaataacgct
     3181 gatttaaata taaacttaga gaaaaaaagt atcgttttag gtagcaaatc aaaagttagt
     3241 gtcaaattta aagaaaaacc agatgcagat agcattacat taaagtataa atgctatgac
     3301 atgccattgg atacaactct aaattacaat caatcaactg gggcatatga aggaactatc
     3361 aattataacc aagacccaga atatctaaat gtttgggaac tacaagggat aacaataaac
     3421 agcaaaaata atcctaaaac tttaaacgga caagacctag aaaagatggg attaaattta
     3481 aaagactata atgtaacaca ggaatgtata attgaagaca taacttctag aaaagatgta
     3541 aataaatatt tgagaaaaac ttctgcacct attacagaac ttacaggaag tgatagatat
     3601 gaaacagcag ttaaaataag taaagagggc tggaaaaatg gttcagataa ggtagttata
     3661 ataaatggag atgtaagtat agatggcatt atatcaactc cgctggcaac cacatataat
     3721 gctccaatac ttttggttga aaaaaacaat gtgcctaata gtgtaaaatc agaattaaag
     3781 cgcctaaacc ctaaagatat aattataatt ggagatgaga atgctatttc taaaactact
     3841 gctaatcaaa ttaaatcgac tgtaaatgct agtcaaacac gtttaaatgg ttctaataga
     3901 tatgagacat ctttattgat agcaaaggaa atagataaaa atcatgatgt ggaaaaagta
     3961 tacataacaa atgctaatgg cggagaagtg gatgcactta ctatagcagc aaaagcaggt
     4021 caagacaagc aaccaattat attaactgat aaagatagta ttacagacaa tacatataaa
     4081 tggttaaaga gtgaggattt acaaaatgct tattttatag gtggtcctca aatgatatca
     4141 acaaatgtta taaataaggt aaatggaata actaaggata gtgttactaa taatagagta
     4201 tacggagcag atagacacga aacaaatgca aatgtaataa aaaaattcta tacagaggat
     4261 gagatagagg ctgttttagt agctaaatca gatgtacttg ttgatgcttt agcagcaggt
     4321 ccattggctg cgaacttaaa atctccaata cttataacac caaagacgta tgtatctgca
     4381 taccataaag ataatttaga agctaaatca gctaataaag tatacaaaat aggtggagga
     4441 ttgacttcta aggtaatgag ctctatagca tcatcattat ctaaacacaa tacgactcca
     4501 acagaaccag gaaatagtgg gggcaagaca gttatgattg acccagggca tggtggttca
     4561 gcacctggaa attcatctgg aggaatgatt gaaaaagatt acaatttaaa tacttcactt
     4621 gcaacaactg aatatttacg ttcaaaggga ttcaatgtaa taatgacaag agacacagat
     4681 aagactttat ctcttggaaa tagaactgct ctatctaatt cattgaaacc agatttattt
     4741 acaagtatac attataatgg ctcaactaat aaacaaggtc atggtgtaga agtattttat
     4801 aagcttaaag ataaaaatgg ggggactact aaaactgtag ctaccaatat attaaataga
     4861 attttagaga aatttaaact tacaaataga ggtataaaaa caagagtact tcctagtgat
     4921 tctacaaaag attatttata cgttttaaga agtaatgata tgccagctgt acttgtagaa
     4981 tgtgcatttt tggataatga aaatgatatg agtttaataa actcatctgc aaaagtaaaa
     5041 gaaatgggta cacaaatagg taaaggaata gaagattcat taaaataaat ggaaataaaa
     5101 atatttaaaa acttaatgta aatttatact aaagtcaaaa aatgaaatat aagtgtatca
     5161 atagtaagac tgtatgtaca tttgcacatg tgaatgcaag tatatataca gtcttttttt
     5221 gtagttaaat gcaataattt gattgtgttt tgtaaaatac acaatatgat ataattagat
     5281 aatatacact taatttaatt gaggaaggtg attatatgga atatttattt gaaaaaagta
     5341 gtttaaaaga atatggaaat gaagctttag ataaatatag agaattatta gataaaactg
     5401 ataataattc aaggtgtatt cttttattag taccaaacaa taacacaaag atacgatatg
     5461 aaagggctct aagattaaat tatagtgaag aactgaaaat aacaacatat ataagttttg
     5521 tgaagaaaga attagttaaa tactggccac taataataga aaaatgtgat gaaataaata
     5581 caaaggttgt atctccaaca tttatatcaa atagtttaag tgaatattta atagtgcaaa
     5641 aagttaagga aaaaaggata caagaaggat attttgaaga tattacagga accaataaat
     5701 ccatagcaaa tagtataatg actaacataa ataaatctgc atttaattta attgattttg
     5761 attttattgg agaaaaaata tattcatcaa agaaaaataa agatagtata tatagatttt
     5821 catacactca gatggatgaa attatatcat attatataaa ttcgttacta tcaaattcaa
     5881 ttttagataa tgcattaagc atatatttat acaatcaata cctacttaat gatgattttt
     5941 atttaaaaaa cttatttaca gaagtgagat atataattgt ggatagtctt gaaagctcaa
     6001 gtaatgcaga agttgatttt ataacagaag cattggataa tacattacaa agttatatat
     6061 attttgatta tagtagggat tattctgtat ttaataatgt ggatatagac tatataaatg
     6121 agaaaataat atcaaaaatt aagcctaaag aagaaaatat taaaaagaag gagattagaa
     6181 tagaagattt atacttatta cctgcaaata tagaattaaa tcaatcaagt caattataca
     6241 atgaaatgtt agatttaata agtgaaaaag ttatatctct tatagaagaa aatgtatctc
     6301 caagagatat agcaattata tctcctataa acaatagtat attggagcat caaattagag
     6361 attctttgat agaaaaaaat atagatgttt ttagtactaa gaaggataaa aaagctgtag
     6421 attatccata tgggaatgca ttggtagtgg ctacatgtat attttatgga tatttagatt
     6481 ttataaaaga tgatgaatac ttaagctttt tagaaactct tttagaaata aataggataa
     6541 aagcatttaa gatttttaat gaaacaagac atttaagtat tgataatata gatgaacata
     6601 gtcagtatcg tgatatttta cagtatattg aagaaaaaaa gaagagtgat ataaaaatac
     6661 atgaattttt aactcaattt tacatagata aaatgttaaa tttaaaagaa ggtaaaaaga
     6721 atgttgggtt atgtaaaaag ataatagctg aaagtgagtc ttttagtgaa agtttaagtc
     6781 tacttaggat gaaaaaagaa aagatattta tagaagcttt aaaaagtaca ataaacgatt
     6841 attactctgt tgtcgatata gaagagttga aatccaaaga caaaatagta ataactactc
     6901 cttattcata tatatcatca aatatagata gaagtataca aatatgggta gatataggaa
     6961 gtaatgcttg gaatatgaaa atagaaaaag atataagtaa tttaattgta cttagaaaat
     7021 cttttgaaga aaaaaagatt tatacaaatg aaatggaaga atattataaa aaatattatc
     7081 tatacaatac agtatataat ttgttattaa atgcaaagaa aatctatgca tttaaaagtg
     7141 aatatgcgat taatggttat atccaagaga gtatattata tagtatactt ttaaaaatct
     7201 cacataaagg agataaaaat tatgattaat ataaattata gagaagacca gataccaata
     7261 ataaattacg ataatggtac tatggcagta ccagcagtac caggtgctgg aaaaacattt
     7321 ataataacaa atttagtagc taaactatta cttgaacaga aacataaggg tggtaaaata
     7381 ttaattctta catatatgaa tagtgctgta aataatttta aaggtagaat aagaaagatt
     7441 cttgaagaga ataaaataga tgatactaat tcgtatgaag taatgactat acacagtttg
     7501 gctgtaaaaa taataaagga aaagccagaa gttgtaatgt taagtgagga ttttaacata
     7561 gctgacgatt tgcaaaaaag tattatatta aatgaatgta taagtagatt tagatttgaa
     7621 ggtggagaaa gagcttttag atggttttta aaggagcaaa aagatgaacg ctggaaagaa
     7681 atagctcttg aagcctggga aagaggtttt tttgaatttg caggaaatgc aataagtgaa
     7741 cttaagtata agggaatttc tccagataaa cttgaagaaa tattgtcaag aggacacaaa
     7801 ggaatgctta aaattatttt accaatatat aaattgtatg atagaaaatt aaagcaaaat
     7861 ggattgctag attatgatga tatattaatt ttagcagaaa aaactttaag cttagacgaa
     7921 gggcttagaa agaagtttca aactagatac aaatatatat ttgaagatga gtgtcaggac
     7981 tcaaatgaaa ttcaaggtaa tataataaaa attatatcta gtgaaaataa aaacctagtt
     8041 agagttggag atataaatca gagtataaca ggaacatttt catcatcaga ccctaaattt
     8101 tttaaagaat ttataaatag tgcagatttc tgttatagga tggatatgtc aaatcgaagt
     8161 tcaaaagata tacttgattt agcaaacact cttgtcaagt atgttacaaa ggaatttaga
     8221 caagaagaat gtagacatgc tcttgaagat atgcaaataa aaactgtgcc aaatggaatg
     8281 ggatataaag aaaatcctaa tccagaacat tataatataa atgtaaagtg gtatgagagt
     8341 tggaaaattg aaatagagca aactgctaga tacgttaagg gtataaaaaa taaatatcca
     8401 gataaaagta taggtatatt ggttccattt aatgaacaag tgacacaagt tgctaaagaa
     8461 cttagagaga ataatctaac ctttgaagaa ttaggaccaa attctttgaa taaaaggaag
     8521 gttataaaca acatagcata tattattgat tttatgttac attgtgatga tgtagaaaag
     8581 ttaatagttg tacttaataa agtttttata gatactgata atgaagaaga aataaagtat
     8641 tttttagaag aattaaaaaa ctattctacc gaagaaatta tttataattt tgaatttagt
     8701 aagttagata atgatttaat agaatttaaa gaagaaagtg acatatattt aagttttaaa
     8761 agaggaatag atattataaa acagatatta gaatatccaa taataaggtt agacctctta
     8821 gttttgttta ttgggaaaaa acttaattta gaaaaagaag ataaagctgt actggattat
     8881 atatcttttt atataaagta tctaatatca gaaaatataa atatggattt agaagatgta
     8941 tataatttat tgtttgatgt aaagaatagg gtgtttaatc atataataga tgtggtatat
     9001 gagataaatg gatatgaacc agagcccggt agtataacac tatgtaacta tcataagtca
     9061 aaggggatgg aatgggattg tgtgttttta ctaggtcttg ttgaatataa tttccctgac
     9121 aatattaatc aaaagtttca gagtgataaa tggtatctaa aagaaaaata taaaaaccct
     9181 atggcaatta ttaaatcaga agttgaagct atattaaaag gaagtatttc aacagattat
     9241 gctcataaaa cgaagattga ttctataaat gaaaagatta gacttttata tgtagggatt
     9301 actagagcaa aggaaatgtt agtactttca ggaagtgctt atagagatga aagtgatata
     9361 gggaataaga gaaaagagca aaaaccatgt atatatttaa gcagacttaa tcaacatatt
     9421 attgaaaaaa ggtcaaatta atatataata agttattaaa agattgcttg aaatatatct
     9481 aaaaggggat aaaacaatat gtacagtaag taactactaa aaaataaagt taagagagaa
     9541 gtgatatgag tgaacgataa attaaaatat ttttcttata gtcaaaattc aatcaacaca
     9601 tataaatcat gtccattgaa atttaaatat aaatatattg atagaataaa ttggaaaaat
     9661 gatgatgttg gtagtagaga gtattatgaa actttaaaaa caggaagaga ttttcatttg
     9721 atttgtgaaa gatattttag caatatacct cttggaatct attttaatga ggatgataaa
     9781 aatagtaaaa agtttttaaa gtggatggaa aatataaaaa aagttgttcc tattgaaaaa
     9841 ggaaaaacgt atttgccaga atatgaagta aggatgacct taaatgggga tataatacag
     9901 gcaaagtatg atttagttgt aattggaaat aacaatatag aaatatggga ttggaaaacc
     9961 gagtctaaaa agattgacta taaacacgta gaaaatagaa tacaaactat tgtttatatg
    10021 tttttagcaa aagaagtaat acctaaagtt ttaaaaatgg acataaatgt taaagatata
    10081 aatatgagat attatcaacc tgaatttgat gacttaccaa ttaccatttt atatgatgaa
    10141 gaaaaacatg aagcaaatag aaataaaatt caaaattata taagtatgat aaaaaacact
    10201 aattatgaag aacatacata cgaagacaat ctatacaatg atatagaaaa ggtatataat
    10261 aaagaagaac gtatgtgcta tagaaataaa aagcattgca agtattgtga atttaataag
    10321 ttatgtaatg gcaaagaaat agattatagt attttggagg cggaaattta tgggacttag
    10381 gtttgtatta ggtagaagtg gaagtggaaa atccacctat atactagatg agattaaaaa
    10441 agaagctcaa aaaaatgaaa cgacatcaat tatactttta gttccagaac agtacacatt
    10501 tgaggctgaa aatagagtaa gtaagctatt cttaggaaaa gaaaaagata agtatctaag
    10561 agttagggta cttagcttta agacactaag taatatagtt ttttctcaag taggaggatt
    10621 gacagatgta aatataaatt ctagtggaaa agcaatgatg gtatatagag ctattgaaga
    10681 tgtaagtgaa gaattaaatg tgttttcaaa atcaaaatcg cagtctggtt ttgtaagctc
    10741 aattacagat atgatatcag aaatgaaaca atacaatata agccctgaaa tgttagaaaa
    10801 tatatctgga gaattggata atgaaacttt aagtttaaaa ttaaaagata taagtaaaat
    10861 atataattct tttgaaggga aattgcatga aaattatgta gatgctcaag atatgttaac
    10921 ttcattggca agtaaaattg aattaagcag ttatttagat ggagcttgtg tttatataga
    10981 tgaatttaca ggttttacac caaatcagta caatgtcata aagagtatat taaataagtc
    11041 taaaagtgtt aatatctctt tgactgtaga tgatatcaac tatattgggt acagtaaatc
    11101 tgatatgttt tctagaacga aatttactta ttcaaaattg actcaattgt gcaacgaaga
    11161 aggcataaaa atattgccac aagtaaattt aaacacaggt gttataaaaa gatttgaaaa
    11221 agttaaggaa ttacagcacc tagaaagatt ttataatgct tatccatata aaatatattc
    11281 aaatccaact gaaaatataa agataaaaga gtttaacaat ttatattcag aggtggaaga
    11341 gatagccaga gaaattgtac atttagttag agataagaat gtaagatata gagatataac
    11401 cattgctaca agagatttaa atagatatga ttttttagta cactctatat ttaatgaata
    11461 taatatacca aactttatag ataagaagag agaggcaaaa agtaatccaa tagttatttt
    11521 aattatttca gctttagaaa tgaagaatag acgatatggg tatgaaacta tgtttaggta
    11581 tttaaaaagt ggacttatag gaattgataa tgatgatata aatttactag aaaattatgt
    11641 gttggctaat gggattaaag gtaaaaaatg gtttgatgaa aaatgggatt atagaataac
    11701 acaaagtctt tcaggtcaag aaagtgaatt tgaactggaa ttaaaagaaa aaataaatga
    11761 aataaaaaat agagtattag agcctatagt aattttacaa gaaaaattaa gaggaaaaaa
    11821 tagagtaaaa gaaatctgta gatatatata tgaattttta ttagatataa atatgccaga
    11881 aactatagaa aatttaatag taaattttaa ggataaaggg gaacttgatg ttgcaaatca
    11941 atactcacaa gtttgggaca tagtagtgga catattagac caaatggtgg aacttatggg
    12001 tgatgaaata atttctttag aaaaatttat aaaattaata accttaggct ttgatgaata
    12061 tgaattagga ctggttcctc ctagtataga ccaggtactt gtaagtagtg tggatagaat
    12121 gaaaaatcca gacacgaaat atttatacct agttggaact actgatggtg tgtttccact
    12181 gattacaaag gatagtggaa tattgagtga taatgatagg gaaagtcttg gaaacaaagg
    12241 aatcgaagtt gatattgata gtaagactag gagttttgaa gaacagtttc ttgtatataa
    12301 agctcttact tcgacatcta agaatttaac cataacatat ccaatatcag accatgaagg
    12361 taaaacgctt agaccatcaa taattatatc aagacttaag aagatatttc caaatataga
    12421 aaataaaagt tatttagtgg aagaaaataa aagcactgat aaagatatac ttaaaaaaat
    12481 aacggttaaa tcccctacat ttaatgaact tataaacgta ataaaaaatt atgacagtga
    12541 tgattacaat acagaagaga ttaatagtat ttggttagat atatataggt actatttaaa
    12601 agatgaaatc tatagttcta taacaaaaaa agttataaaa ggtcttagtt atactaatca
    12661 agttcataag attgaagaga aaaaaataag gtcattgtat aaaagtaatt ctcttagcat
    12721 atctaggctt gaaaaatatg ctgaatgtcc atttgcttat tttatacaat atggtctaaa
    12781 agcaaaaaag cgtaaagagt attcatttac accaccagat ttaggaactt ttattcataa
    12841 tattttagat aggttttcaa aagaactttt acaagataat ttaacttgga gagatattga
    12901 tgaaaaatat atagaattaa agataggtat aatagttgat gaaataattt taaagatacc
    12961 aggatatata ttaaatagtt ctgagaggta taaatacctt gcatatagat taaagaatat
    13021 gttgacaact gctataacta taattagtca gcaaataaaa caaggttcat ttgaaccaat
    13081 agattatgaa gttaaatttg gcgacaatgg aaaatatcca cctataaaaa tggtgttaga
    13141 aaatggacaa gaagtaagtt taattggaca aatagataga gttgatgaat ttgaagaagg
    13201 agaaaataaa tatataagaa taatagatta taagtcaggc aataagtcca taagtctaac
    13261 agaaatatat tatggtctac aattacaatt acttgtgtat ttagatgcaa tccttgaaag
    13321 tgcaaaggat gaagatataa atataaatcc agctgcaata ctatattgta gaataaataa
    13381 tccaatagca aaatttaatg aagataaaga tgatgaagaa atacaagaag caatattaaa
    13441 agaacttaga atgaaggggc ttgtagtaaa agactctcac atagttaaag agatggataa
    13501 atcattaata gatggagaaa ggaaaaactc tttagtaata ccagtaggtc ttactaagga
    13561 tggaaatgta ggtaaaagta cttcagcaat aagctatgag gatttcaaac tgcttagaaa
    13621 atatgtaagg catgctataa aggatttatg tgaagaaatg ttaagtggag aaatacgtat
    13681 agctccatat aaacataaag atggaacatc atgtgacttt tgtgattact ctactatatg
    13741 tcagtttgat tcaactataa aagataacaa gtataaaaat ttaaataata agagtaatga
    13801 agagataata aaaatgatga aaggagatgt taattagtga gttctcctaa atggacaaaa
    13861 gagcaattag aagttatagg aagtagagaa tgcaatttat tggttgctgc tgctgctggt
    13921 tcaggaaaaa cagccgttct agttgagcgt ataattcaaa tgataacaag tagagaaaat
    13981 ccaattgata tagataagtt attagttgtt acatttacta atgctgctgc gtcagaaatg
    14041 agagagagga taggggatgc tataggaaaa gccttagatg aaaacccaga aaataaacat
    14101 ttacaaaacc aacttgtttt attaaataaa tctagtatta caacaataca ctcattctgt
    14161 ttggatgtta taaaatcaaa ctttcataga ataaacttag accctaattt cagaatagga
    14221 gaccaaactg aatgtgctat tttgaaacaa gaagcaatag aggaagtatt tgaagattta
    14281 tatgaagaaa gagatgaggg gtttttaaat ttagttgaaa gttatgcaga aagaggagga
    14341 gataaagaag tacaagatat tatcttggga atatattcat ttgcaatggc ttctcctgaa
    14401 cctaaaaaat ggttgattga ctcagcagaa cgatttaata ttgatgaaaa ttttgatttt
    14461 tcacagtcta tttgggctag agctatatta gacactgtaa aaatagaaat taatggtctt
    14521 tgtctaaata tggaaagggc attaaaagaa gtggaatcaa tagaagaatt agaaactttt
    14581 gcagaaaagt tatccgttga atataaaaaa attgctaaca tttcacaagc atgtaataaa
    14641 tcttgggatg aggcatataa aaagatggca agtatgtcct ttgaaaatta tgtaaaagga
    14701 gtaaaaagaa tttcaaaaga tgctccttcg tatataaaag aatcaaaaga aaaggcaaaa
    14761 actataagag ataagactaa gaagtcttta gaaagtatag taagtgctac attcaataaa
    14821 gataatgatt ctattagaga agaaataaaa tatctttata acatagttaa accaatatct
    14881 agtgttgtac tgagatttga agaggaatat agcaataaga aaagagaaaa aggaataata
    14941 gattttaacg atatagagca ttttgcatta aatatactta cagatataga tgaaaaaggt
    15001 aatattgtac cttctgatat agccgttgga tatagaaaca agttttatga aatatttata
    15061 gatgaatatc aggatagtaa tctagtgcag gaagtactgc ttaaagctgt tgcaaataca
    15121 gaaactccaa atagatttat ggttggtgat gtaaaacaga gtatatatag atttagacaa
    15181 gcaaagccag aattattttt gcaaaaatat aataactata atgataaaaa gggaagttcc
    15241 catagaaaaa taatgcttta caaaaatttt agaagtagag aagaagttgt agatgctgta
    15301 aattatatat ttgaaaatat aatgaatgag aatataggag aaattgaata tacagaaaaa
    15361 gaaagactaa atttaggtgc gaattttaat gtggatacag acgagaaatc tattattggt
    15421 ggagcaacgg agatacacct aatacaaaaa gataataaac ttgatgatga tattataaat
    15481 gataaagacg atagaattaa taacaaagaa aatgaaatag aggaagaaga aaaattagat
    15541 aatatacaac ttgaggctag aatggttgga aacattatca aagatttaat gaaagtcaat
    15601 gaagatggaa aaattcaaaa ggtctatgat aagggaatag atggatatag acctgtggaa
    15661 tttagagata tagttattct tttaagagct acatctgcat gggctccagt gtttgcagat
    15721 gaattgatga atatggatat accaacatat gctgatgttg gagtggggta ttttgatacg
    15781 attgaaataa aaacaatatt gtcattactt caaataatag ataatccaat gcaagatata
    15841 cctcttatat ctgtattaaa atcacctata tttggattta caccagagga tttgattgat
    15901 attagagttc agagtaaaga taaaattttt tatgaggttt taaaaagtac agcagagtat
    15961 gatggattta cagattctca aaatgaaact gagtctgagt ttataccaag tgaagagtgc
    16021 ataaacaaaa gtaaagattt tttaattaag ctaaaggaat ttaaagaaaa gtcaatgtac
    16081 atgagtacag atgaatttat atggtacttg tacacaagga ctggatacta tgcttatgtt
    16141 ggagcattgc caggtggaag tcaaagacaa gctaatttaa aagtattgtt tgagagagca
    16201 aaacaatttg aagaaaccag tcttaaagga atattcaact ttgtaaattt tatagagaaa
    16261 ttaaaaaaat ctagttctga tatgggaagt gcaaaaacac ttggtgagaa tgcaaatgtt
    16321 gttagaataa tgagtattca taaaagtaaa ggtctagaat ttccagtagt catatgctct
    16381 gcaatgggta aaaatttcaa tactcaagat tttaaaaaga gtattttata tcatcataat
    16441 ttagggtatg gacctcaatt tgtagactat gagagaagga tttcttttcc aagtatagcc
    16501 aaagaagcat taaaaagcaa aataaatata gaaaatttat cagaagagat gagagtttta
    16561 tatgttgcat ttacaagagc taaagaaaaa ttgattataa ctggttctac tagaaatata
    16621 caagatagta taaagagatg gtcaaatgga attgaaagtt tagatacaat atcacaatac
    16681 gagatattaa aaggtaaaaa ttttttagat tggataatgc cttgtgtatt aagacatagg
    16741 gatttatcta atctactaga agaagtagga ttagacgcag tttttaatgt tgaacataat
    16801 tcaaaatggt atggaaagtt gtggaataag aatgatattt tagtggaaaa gaagagtgat
    16861 gaagaaaaag aaagtattga agaaattctt gaaaaaatag atgtagatag tccggacagt
    16921 gattattaca gtgaaataga aaaaaaatta aactatatat atccatatga atttagtaca
    16981 agaaaacctg ctactatatc tgttacagaa ataaaaaaga tacaaaataa ttatgaagaa
    17041 gagttaataa atactatatt tgaacaaaaa gtaatattaa aaaagcctct atttattcaa
    17101 aatgaagaag aaagagaaaa aataagtgga acagaaagag gtacaatagt ccatttggtt
    17161 atggaggttt tagacttaaa aaatgtaagt agtgtaaatg atataaaatc acaaataaga
    17221 ggttttgttt caaaaggtat aataacagaa aaacaagcta gtatagtaaa tccatataaa
    17281 atatataaat tttttgcctc taacatagga aaaagaatgt tgaatgctga aattataaat
    17341 agagaaaaat caatttatgc acaagtaaat atgaaagaca tatatattta tgaaaagctt
    17401 ataaacaatg atgataaaaa actttatgat aatgaaagtg tcatgttaag aggtatagtt
    17461 gatgcttatt ttgaagaaga taatcaaata gttttggtgg attataaaac agattttgta
    17521 aatgaggaaa atataaatca aataatagag aaatataaaa aacagttgga tttatatgca
    17581 gatattattg aaactttaac tggtaagtca gtaaaagaga aatgtatata tttatttgga
    17641 gttgatgaag ctgtttgtta ttagttttta tatataaatt aaacctaaaa gatagaggaa
    17701 ggggaaaaaa tgagatttat tcatacatca gattggcatc tagggaagtc cctagaaggc
    17761 cattctagaa ttgaggaaca agcaaagttt tgtgaggaat ttataaaaat agtagaatca
    17821 aatgaaatag atatggttat aattgctgga gatgtatatg atacttctaa tcccccagca
    17881 caagcagaaa aactatttta tcagactgta tcaagacttg ccaataatgg tcagaggtgt
    17941 gtattaataa tttcaggaaa tcatgataat ccagagagac tttctgcaat aacacctcta
    18001 gcacatgaac aggggatact tatatatggt tatccattaa gtgctacgat agaagctaag
    18061 tataaagggt ttgaaataac atatgcaact cagggatgta caaaactgaa tataaatgga
    18121 gaaaatattg tgattgctac attaccttat ccaagtgaaa aaagattaaa tgaagttttt
    18181 tcaagtgaag atgagtttga gaaacaaaaa aattattctg aaaaggtagg agatatattt
    18241 agaagtttag aagaaaattt tagaagtgat actataaata tagccgtatc acatatattt
    18301 gtaataggtg gagagagtac tgaatcagaa agacctatac aattaggtgg aagtttttta
    18361 gttgaaagaa aagatttgcc agaaaaggct caatatactg cactaggtca tttacataaa
    18421 caacaaaaag cttccgaacg tttaaatgca tactattctg gttctccact tcaatatagc
    18481 aaggatgaaa gagcgtatac caaaggggca tacatagtgg atataaaagc tggagaaaaa
    18541 ccaataattg aagatgtata ttttaataat tacaaaccta tagaagtatt taaatgtaat
    18601 ggaatagaag aagcactaga tatatgtgag caaaatcaag atagagagat ttggtcatat
    18661 tttgaaataa atactgatga gataatatca cagaatgaaa ttaaaaaaat gaaggaacta
    18721 ttaaaagaca taatagaaat aaaaccaata ataacatctt gttatgaaca agaaagtgta
    18781 gatataaaag aaaagtctat ggcagagttg tttagagagt tctattcttt tagtaaaggt
    18841 gtagagccaa aaggagaact tatggattta tttctagata taataagtga agaaggtgaa
    18901 tcagcagatg agaccaatta gattagaact tacaggactt aatagctata ttgataaaca
    18961 agttatagac tttgaaaaat taattgaaag aggtttgttt gggatatttg gaactactgg
    19021 aagtggaaaa tctacaatct tagatgcaat aactattgct atgtatggaa atatatccag
    19081 aaatacaaaa gaatatataa acagtgtgtg cgataaggca attatatcat acgagtttga
    19141 gataggaagc aaaaacacta gaagaagata cattgtagat agaaccattg ctagaagcaa
    19201 aacaggaaca aagacctcta atgctagatt aatagaagta ttgaatgaca atatccaaaa
    19261 tgttttagct gataaagttg tggaagtaaa tgaaaaagta gcacaggttg taggattaac
    19321 ggcaaatgat tttactaggt cagtagtttt accacaagac aaatttaatg agtttctaag
    19381 attatcacgt gcagatagaa gagacatgtt agagagaatt tttaatcttg agaaatatgg
    19441 aagaagtctt ggagaaaaag tgaaaaagag aaaaaatata cagttacaaa atttaaaaga
    19501 tttgaaatca aaattaagtc aatatgatgg aattacagag gaagtataca acaatataaa
    19561 ccaagaatta atagagttaa aaaatttgga aaaagataag aataatgctt tagacctagc
    19621 tcaaaaatct tatgaagaaa gtaaaacagt atatgaagaa caattaaagt tagaaaaaaa
    19681 tgaacttaga aaaaatgagc ttgatttaaa aaatagtgag ataaaagaaa agattgctct
    19741 tgtagaaaat gatagtaacg ctaaaaaggt agacccatac ataagttcag ttcaaaatct
    19801 agaaaaaaag atagatgaag atagctttgt agtaagtaat ttagaaaaga aattagcaat
    19861 attaaatcag gagcttgaag ttacaaaaaa tagatatgaa aaaataaaca aaattaagaa
    19921 tgaagaagtc cctaaattga gtgaagaaaa aataagattg caacaagcaa taaaattaga
    19981 agaagagtta gttttattag atagagagtt aaaagatcta aaagaaagtg gaattaattt
    20041 aaataagact aaagttgaat tagaaaaagt aaagcaggtg tcagagtcca gaaaagatgc
    20101 agttactaaa tctataaaag aagttgaagg aaagattgat aaagtaaata taagtgcaga
    20161 attaaaacaa aaaatatttc tagcatatga atacgagaaa gattacaaca acgtattgga
    20221 agaaaaaaat caaaagctaa ataaactaga agaaatactc aaagagacag aaaatataaa
    20281 tttaaaagtt agatatattg ataaagataa gaatgatgta aatcgtaatt tagaaaattt
    20341 atcacttcac ttggatgttc ttcttaaaaa atgtcctggt aagagtgctg atttactttt
    20401 aaaatctgaa tatgttactg aattaagaag taaagcaaat aatactaaag aaaatgaaat
    20461 taagaaaagc agtatacaag atgaattaaa gataatactt gaaagtaagt ttaatactga
    20521 aagggaagta aatttactaa atgaaaaact tgaaaataat agaaagaata gagatgacct
    20581 agaaaaagaa ttagaagaac taaagtattt aaatttagcc tctgaactta gacgtgaatt
    20641 aaaagaaaat atgccttgtc cagtttgtgg gtcaaaacat catgaaaatc ataatataac
    20701 taagtatgat gaaaatatat cttttgtcaa agaaaaactg gagaaattag aaaaagaaaa
    20761 aataagtata agaaacaata tagaggaatt aaatgctaaa gtaagtgggt atttatctat
    20821 agaaaaaatg aagactaaag aacttgaaga tgtaaaagga aaactagggg ggattccatc
    20881 tagtcaatta ttaaaaaaac ttgatgaaga acaaagaaaa cttgcattgt taaaatctaa
    20941 tattcaggag tgggagaaag aaaaagagag tactgaaaat aaagtaacat tagctaaaga
    21001 agaaaaaaat aaaattgaaa aagaagaatt aaaaataaga gagagtctga ataattataa
    21061 gaaattaaca agagatttaa acatagaaat agaaggttta gaaaataaat gtaaaaaact
    21121 taaacaggaa tacctaggat taaagacgat tactaaagtc tctaatctat tgtcaaaagt
    21181 aaaagaaata agggagaatg aaaaagaatt agaattacta agtactgggc attcaaattt
    21241 attaaaaaat agagactcac tagatattaa aataagagaa aatgagagta aactacatga
    21301 aatagagata gaattaataa aggctagaga attatatgta gaaaagaaat tgagtagaga
    21361 taataagtat aaagaagtta ttaacataac gaaaggtgat ttagctaaaa atcttttaca
    21421 taatgttgaa gaaaatatat gtaagatatt agaacaagaa gaatcaagta agaaaaaatt
    21481 ggaagagcaa agattagaat atgaaaaaaa cattgctgaa aagcacaata tagatggtag
    21541 acttaaaact gctaaggaac aatataaaga ccaaaaatca acactaaata aactattagc
    21601 agaaaataaa tttgaaagta tatatgcagt aaaaagagct ttattagata ctgatgcagt
    21661 aaaaaaattg attgaagaaa tactagagca tgaagaagaa caaaagttat tatcatttaa
    21721 gataaaatca tcaaaagaaa aattaaatgg aagaagcata aagaaggagt actttgaaca
    21781 gttaaaagat gaaatttata atttgaaagt agaaatagga aatatatcca aagatattgg
    21841 agctaatcaa aatcagttaa taacactaaa agactcttta gataagataa atgattttaa
    21901 taaacaactt aaggtagttg aacacaatgt ggacttattg gaagaattag ataaatgtgt
    21961 gcaaggaaat aaatttgtag aatatgtttc tacaaaccaa ttaaaatata tagcattgga
    22021 agcatcaaaa cgattggatg gaataacaaa aggaagatat gcacttgaaa ttgactcaac
    22081 actaaacttt gtaatgagag ataactttaa tggaggagaa agaagaagca ttgatactct
    22141 atctggtgga gagacattct tgacatctct atcattagca cttgctcttt catctcaaat
    22201 acaattaaag ggaagtgcac cattagaatt tttcttttta gatgaaggtt ttggttcatt
    22261 agatagtgaa cttttagaaa ttgttataga atccttggaa agattgcata gtaataatct
    22321 aagtgtgggt ataataagtc atgtcgagga acttaaaaat agagttcctg ttaagctatt
    22381 agtttcatct agtgaggctg gaataggttc aaaagtaaaa attgaatata gttagaaaat
    22441 atatggtaat tgaatatatt atctaatatt tccaaataat atgtaattgt gatataataa
    22501 aaactaatat taaagttatt tataattaat attaatataa aaacatgtaa gtaggagtgt
    22561 ggtaagtgtt ggaatcgccc aataatttga ttcagattat ttttttaata gtattactta
    22621 tagggtctgc atttttctca gcatctgaaa cggccttaat gtcgttgagt aaaatcagaa
    22681 tcagatatat gcaagatgaa ggagttaaag gagctaagtt agtaagttca ttaatagaaa
    22741 atccaaataa gcttttaagt tctatattgg ttggaaataa cgtcgtaaat atagcagcaa
    22801 catccatatc aacatctttg tttataggat taatgggaga aaaaggagtg gcacttgcta
    22861 cagctgttat gacagtatta gtattaatat ttggtgagat tactccaaaa acaatagcag
    22921 caaataattc agaaaaggtt tctctattag tatctaaacc tataaaagca attatattta
    22981 tattaagacc aatagtatgg atatttaata taattacaaa tattatattt aaattatttg
    23041 gtataacaaa taaaggagct aaatcattca taactgaaga agagctaaaa actatggtaa
    23101 atgtaagtca tgaagaaggc gtccttgaaa tggaagagag agaaatcata aataatgttt
    23161 ttgaatttgg agatatgcaa gctaaaaatg caatggtaca aagaatagat atggtagcta
    23221 tagatatgga agatagttat gatgagataa tacaggtatt taaaactgaa aaattaagta
    23281 gaatgcctgt gtatgaagaa actatagatg atattgtagg aatacttaat ataaaagata
    23341 ttatattttt atctgatgaa gaaatagagt catttgatat taaaaattat atgagagaac
    23401 cttttttcac gtatgagttc aaaaagataa cacaacttct tgaagagatg aaacttgaaa
    23461 aaagtcagat ggctatagtt gtagatgagt atggtggaac atctggactt ttaactatag
    23521 aagatttagt agaagtcatt gttggagata tagaggatga atatgatgaa gaagaggatg
    23581 aaatacaagt tataaaagaa gatgagtata ttgtagatgg aagtactaaa attggtgatg
    23641 ttaatgaact tattggtgta aacttggaat ctgaggagtt tgattccata ggaggtttta
    23701 taattggaca cctaagcaga ttgccagaag aaaatgaagt aattgaagtt gataatataa
    23761 gattttgtat agaaagtata gaaaaaaata gaattaaaaa aataagaata tatacataat
    23821 gtaataatgt tcttaaattt attagaagtg tataaatata aaagatatct tttatattta
    23881 tacacttctt tttattttgt attcaaataa aactagtatg aaatggacaa aataaaaata
    23941 taaattagaa gaaaggagat ttttaagatg agcatactag aaaataattt tttatacaaa
    24001 ttaaaaaaca acactatctt tttttgtata tatacattgt tatttatttt tatttacaaa
    24061 gcgtttccat acatagctcc attttttcta ggtgctataa tagcacttat gattaatcct
    24121 atatcacaaa agttagaaaa taagtttcgt ataaataaag gtatctctac tcttgttcta
    24181 agttttcttg cagtggctat agtcagtaca gttactacaa taatagtaat aaattcaatg
    24241 aaagaattaa tgggattttt aaataatata tctgctaatc ctgaggatat aagtaatact
    24301 attatgtatt ttttaaataa aataaatgac tttatgaaat catttcaaga aatagccaat
    24361 tttgatgtag aacaattagt aaacaaattc agtggtgaag taatgcaaat tacaaaaaat
    24421 cttcttacta gtatacttgg attggctact tctattccat atattataat atttataatt
    24481 accttgttta ttgctaccta ctttatagca aaagaccttg ataagattga aaatagtttt
    24541 tataatatgt ttactgttga tgttagaaag aaggtaaaaa acgtaaagaa agaggcaggg
    24601 ctttcacttg ttggatatat aagggcctat acaatactta tggctataac attttttgca
    24661 atctggggaa gttttgcttt atttggattg aaatacggtc taatagtagg gtttgtaggg
    24721 ctttaatgga tttaattcca ttcctaggta taataacaat atatcttcca gtaattgttt
    24781 attattttct tataaaaaat tattttatag ctataagtat gactgtgata ttttttgttt
    24841 tgtctttgat tagagaaata cttgaaccta aactagtatc agtaaatgtt ggactaaatc
    24901 cattagctac cctagcagcg atttttattg gaatccaagt aaaaggaata attggagtaa
    24961 ttttttgttt gggtttggta tgtatgcatg atatacttaa gaaggtggat attttttaat
    25021 tataataatt gatagatttt tttaaaaata aaattttatt cacaggtaaa taaaaaaata
    25081 ttaatcttat taagaaagag ggtatatacg tgaaacaaaa agtagtggaa agatttttaa
    25141 aatatgtatc atttgataca acatctaatt ctcagtgtga gaactgtcca tcttcagaag
    25201 gtcaaagagt tttagctaag tatatagttg aagagctaaa aacaatgggt gttgatgatg
    25261 tgagtttaga tgaaaatagt tatataatgg ctactttaaa aggaaatact gatggtgtgg
    25321 atactatagg attcatatca catttagata caattgaaga tgttagtgga aaagatatta
    25381 aaccaagaat aatagaaaat tatgacggaa aagacatagt tttaaatgaa gcgttaaatg
    25441 ttattacgta tgtaaaggat tctccagagt tggaagaatt taaaggtgac gatttaatag
    25501 tcactgatgg aacaactcta cttggctctg atgataaagc tggtatagct gagatagtga
    25561 ctgcaataga gtatctaata aatcatccag aaattaagca tggagatata aaaataggat
    25621 ttacaccaga tgaagaaatt ggtagaggtg ctgatttatt tgatgttgaa aaatttggag
    25681 caaaatatgc atatacttta gatggaggaa tagttggaga acttgaatgt gaaaatttta
    25741 atgctgcaaa tgcaactatt actatacatg gtagaaatgt tcatccaggt tcagctaaaa
    25801 ataaaatggt aaatgctata catatagctg ctgaaatatc agaaatgttc ccagcagatg
    25861 aaagaccaga aacaacagaa ggatatgaag gattttggca tttaaattct ataggtggaa
    25921 atgtcgagaa tgtaagtatg gcttatatta ttagagacca ttgtagagaa aaatttgaaa
    25981 atagaaaatc tattatgata gagaatatag aaaaaataaa taagaaatac gataatagag
    26041 tggaattgga tttaaaagat tcttattaca atatgaaaga aaagattgag ccagttatgt
    26101 ttatagttga tatagctaaa gaagctatgg aggaattagg tataaaacca cgtttagttc
    26161 ctgtaagagg tggtactgat ggagcaagat tatcttttaa tggattacct tgtccaaata
    26221 tatttactgg tggattaaac ttccatggga aaaatgagtg tataccagtt agttctatgg
    26281 agaaagctac taaactaatt gtaagaatag ctgaaaaata tgctgaaaga gtttagttaa
    26341 aaagtattta aaaattaagc atttaaagtt ataatagcta tatcattctt tataagtcaa
    26401 gctgattata agcttgtgtt gtatttaaaa tggtatagct atttttgtaa ttatttttat
    26461 aaaagataat tatttttagt ttagggtatc aataaaaagt atggatatca ataggtatta
    26521 acatttaaaa gggggaattg tattgaaaat taaatctaaa aaaataatag catttgcact
    26581 ttcacttggg attatgttgc caagttgtgt atatgcagac actattagta aaaatcaaaa
    26641 tgcacaggta gatatgaaca attatcttca aggtagaaaa tctccaaagg aggaaaaact
    26701 tattggtgaa gatagatttg atacagcgat aaaaatatct caaagtggtt ggaataatgg
    26761 ttcagaaagg gtgtttctag ttaactcaaa ttcattgcca gatgcattgg cttcaactcc
    26821 tttggcaagt aaattggatg caccaatact gcttacaaat aaaaatagta taccatataa
    26881 tgtaacagag gaaataaaaa ggcttaatcc agcagaagtt attttaattg gaagtgaagg
    26941 agctatttca tcaagtgtaa aagaatctct tgaagatatg gaagtattag tcagtagaat
    27001 cggtggagca gatagagaag aaacatcttt acttcttact agacagctag atgatactgg
    27061 tgatttaggt gtctcaaaag tggcagtggt taatgggtat aatggactgg cagatgcaac
    27121 ttcaatatca agcccagctg caagtgataa tacagctata atatatacag gtaaggatag
    27181 cataagaagt gaggcaaaga attttattac acaaaactca actaagacat acatagtagg
    27241 tggggagtac agtataagta aaaaacttga aggacagtta gtaaactcag aaagactagc
    27301 aggaacagat agaaaagata ctaatgcgaa agttttagaa aagttttatg gaaaatcaag
    27361 taaggttaat aatatgtatt ttgcaaaaga tggaagtggt agagaagctg atttagttga
    27421 tgggcttgct gctggagtat tagcatcaaa aacaaagtca cctgtagtac ttgcaagtgg
    27481 aagtttaagt agtgcacaga aaagttttat aaaaaaagta aaggctgata aatttgtaca
    27541 agttggtgga ggtaaaaata gtaaaccata tgcagaagca ttagccttac agtaatattg
    27601 tgtaaatcaa aattaatata taataatagc aagtaaacca agttgaactt caatatatat
    27661 aataaaataa gtaaactaag ttatatttca gtat
//
LOCUS       DAELXX010000049        27247 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.049, whole genome shotgun sequence.
ACCESSION   DAELXX010000049 DAELXX010000000
VERSION     DAELXX010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27247
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.049"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            65..886
                     /locus_tag="KPH29_001947"
     CDS             65..886
                     /locus_tag="KPH29_001947"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HBG1467542.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRQEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDDNILNLAYDRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            complement(962..2350)
                     /locus_tag="KPH29_001948"
     CDS             complement(962..2350)
                     /locus_tag="KPH29_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HBG1467543.1"
                     /translation="MLRSKKSNNKKVEKLRTIFIKYLSLFFVMTISFALLLMLSFSVL
                     LSSGVILPANYSEKQFDKYKEKIISSEKAIEDIIPNLYEYGVYTLDGNLISGTFNKKE
                     SKQVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKLVSEYSSPVLRAYLPKPEMLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNILF
                     SMDKMKLALKNSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAINMSEFIKDVLLQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVSNKYLKFVV
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGILYIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(2494..3252)
                     /locus_tag="KPH29_001949"
     CDS             complement(2494..3252)
                     /locus_tag="KPH29_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBG1467544.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSGVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFTSSFYMISILILIGCNLFEYILHLFLSLRFGKG
                     TSIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLMCGFVTILAFVILCIWFSKWEGKKSEE"
     gene            complement(3254..3970)
                     /locus_tag="KPH29_001950"
     CDS             complement(3254..3970)
                     /locus_tag="KPH29_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBG1467545.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLISSLIVLKDEKSKYRGILPLPIEMKDVWAGKMIYCIICCSIASIIS
                     AIGVVFVGIIYKETISIGQNIIATILIILLFMWQIPLCMFLASKFGIFVTVLTNMFFT
                     IVGVAVFSTTEMWYVFPYSIPSRLMVVVLHILPNGLPVPEGSELLNNAVILPGVLISV
                     ILFVLFALVTRAWFNNREVK"
     gene            complement(3972..4679)
                     /locus_tag="KPH29_001951"
     CDS             complement(3972..4679)
                     /locus_tag="KPH29_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBG1467546.1"
                     /translation="MNNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRKLIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVIGYQGEVSKEQN
                     LEKLFMKVVAENRKRGE"
     gene            complement(4796..5332)
                     /locus_tag="KPH29_001952"
     CDS             complement(4796..5332)
                     /locus_tag="KPH29_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG1467547.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            complement(5434..6504)
                     /locus_tag="KPH29_001953"
     CDS             complement(5434..6504)
                     /locus_tag="KPH29_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBG1467548.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAEFKK
                     ENKLYQEKVNIDKLDISVDNINVTIKKSSSEDVRVTTRGNNEFYKYNVTLKDKTLVVK
                     GERKYENKIKKIKNFDQLLNTSINSMFSHDYREIIIYVPNNVDINASSTSSYLFVYDN
                     VASNTITYKTSYGGFSRAITENKVNRLENLNLISNSNLHLSTKSILGVKNVNIESESL
                     DISSENEDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSNMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHSEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            complement(6497..7555)
                     /locus_tag="KPH29_001954"
     CDS             complement(6497..7555)
                     /locus_tag="KPH29_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBG1467549.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNIKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSVTIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINEDSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLEDIENKKGEDKHE"
     gene            complement(7542..7874)
                     /locus_tag="KPH29_001955"
     CDS             complement(7542..7874)
                     /locus_tag="KPH29_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG1467550.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            8073..9194
                     /locus_tag="KPH29_001956"
     CDS             8073..9194
                     /locus_tag="KPH29_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HBG1467551.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLEEFKTVIHPEKIADYISELIDNHYS"
     gene            complement(9322..11220)
                     /locus_tag="KPH29_001957"
     CDS             complement(9322..11220)
                     /locus_tag="KPH29_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HBG1467552.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
     gene            11535..12311
                     /locus_tag="KPH29_001958"
     CDS             11535..12311
                     /locus_tag="KPH29_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBG1467553.1"
                     /translation="MEYIIFDLEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYVKPTIYKDIHPFISRMTNIKNSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGVGDLKELYRNINYHKLPSSSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKMSYHNALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMGKTNQFTLENVKQRKNK"
     gene            complement(12403..13155)
                     /gene="xth"
                     /locus_tag="KPH29_001959"
     CDS             complement(12403..13155)
                     /gene="xth"
                     /locus_tag="KPH29_001959"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBG1467554.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(13316..14266)
                     /gene="galT"
                     /locus_tag="KPH29_001960"
     CDS             complement(13316..14266)
                     /gene="galT"
                     /locus_tag="KPH29_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBG1467555.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIESEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
     gene            14651..15847
                     /locus_tag="KPH29_001961"
     CDS             14651..15847
                     /locus_tag="KPH29_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG1467556.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
     gene            complement(15959..17305)
                     /locus_tag="KPH29_001962"
     CDS             complement(15959..17305)
                     /locus_tag="KPH29_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG1467557.1"
                     /translation="MTMKKRYNIAIVGGGSTWTPGLLKSLCKMSDRFPLNKVTMLDVV
                     EERQRLIGEFGKILFKEEYPKATLEYTTNPDEAFIDVDFVFVQMRTGGLKMRELDEKI
                     PLQFGLVGQETCGAGGFAYGLRSIGDMIEMVKTVRKYSPKAWILNYTNPAAIVAEALK
                     RVFPDDKRLLNMCDQPVNLLRSYGRLLDMDSRTFEPVYFGLNHFGWFTHLYDKNGEDL
                     VPKIKDLVANSGFQPVDAEQRDKSWLDTYGMVKDMLEDVPDYLPNTYVQYYLYPDKKV
                     AKSNINCTRAREVMNGREKRVFEECKSVIEKGTSIGSNLVHNDAHGEFIVEVAEAIAH
                     NKHQIAIVITENNGAINNLPDDAMVEVAAILTKNGPRPLHVGNIPTFYKGLLEGQLAY
                     EKLAVDAYFEQSYEKAILSLTLNRTIISPTKARQVLDALIKVNKDYWPTLYKENEK"
     gene            complement(17438..18190)
                     /locus_tag="KPH29_001963"
     CDS             complement(17438..18190)
                     /locus_tag="KPH29_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG1467558.1"
                     /translation="MNLIDKLDINQKKLSTNEISLLNYFLKNSEKINQKKIKDIANET
                     FNSTAYIVRFCQKLGFSGYSEFKNFLVFSYSNRNERNKEFTKSQSDIFTDVMDTQNLI
                     NVENIDMCLNEIHNSKHIYFFGVGSSRLVCTEMSQRFSAIGVNVKYYDDSTLMYLAAS
                     NITKDDLVIAVSMSGETAQVIKACNIAKTNQVKVISLTNISVNSLSNIADIKLFVSSP
                     QYNFNSVNYTSRVPALSLMEYMFHKYIEKYNR"
     gene            complement(18349..19926)
                     /locus_tag="KPH29_001964"
     CDS             complement(18349..19926)
                     /locus_tag="KPH29_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG1467559.1"
                     /translation="MKQKMKEFFLQLGKSFLLPIALISAAGIFLGISSAFSNPNIVSD
                     IPFLENNFIQGILGFTKAITGVLFGNLAIFFAISISVGLAKEEKAVSAFSGFVGYLVL
                     HTSVNYMLGCRGILAEPDMMREMGQAVTLGIQTLELGVFGGIVVGIITASLHNKYYNI
                     KLPDYLGFFGGTRFVPIITTVVFSILGIIIPYIWPYVNTIIQSVGIGIASLGYLGTFV
                     FGFLERLLIPFGLHHILNAMFRFTEVGGSLVVGGQEVFGALNIFLAQLSDPETISFST
                     EATRFLAQGKIPIMVFGLPAAAYAMYKTAKPENRSKVKGILLAGALATFVTGITEPLE
                     FSFLFVSPILFVIHAFLSGISFMMMHIFGVAIGNTQGGIIDLVVFGVMQPNTKWIYSI
                     ILGLVYALVYYNVFKVLILRFNLQTPGREEITDINFDLEPLSNKVEIAQSGETTKRAL
                     SIIKYLGGKENIEDITNCITRLRVVVKDMSKVDENAFKKYTGAMGVIKVSDKDIQIIY
                     GPSVSQVRDEVSFAMSI"
     gene            complement(20339..20791)
                     /locus_tag="KPH29_001965"
     CDS             complement(20339..20791)
                     /locus_tag="KPH29_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG1467560.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEE
                     GDKIATDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
     gene            complement(21102..22547)
                     /locus_tag="KPH29_001966"
     CDS             complement(21102..22547)
                     /locus_tag="KPH29_001966"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBG1467561.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINKRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
     gene            complement(22691..23599)
                     /locus_tag="KPH29_001967"
     CDS             complement(22691..23599)
                     /locus_tag="KPH29_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG1467562.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
     gene            23856..24680
                     /locus_tag="KPH29_001968"
     CDS             23856..24680
                     /locus_tag="KPH29_001968"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBG1467563.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILINEFIKLNF
                     SNTFINTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRVFFNSTVVAIALIVYGIIMIGLEKRNKRPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            24720..25250
                     /gene="lepB"
                     /locus_tag="KPH29_001969"
     CDS             24720..25250
                     /gene="lepB"
                     /locus_tag="KPH29_001969"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBG1467564.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSVVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDEDEILG
                     KVMVRLLPLDNIGKVD"
     gene            complement(25332..27113)
                     /locus_tag="KPH29_001970"
     CDS             complement(25332..27113)
                     /locus_tag="KPH29_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HBG1467565.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
BASE COUNT         9201 a         4318 c         3130 g        10598 t
ORIGIN      
        1 aatatatatg tatataaata ttttggtatc attataatta atacatacta atggagggtt
       61 ttttatgcaa aaaaaagtcg cagcaattaa tgacttatct ggaataggaa aatgctcatt
      121 atcggttgct atacctatac tttcagcatt aaaggttcaa tgttgtccat ttccaacggc
      181 catactttca agtcaaactg gttatcctga atttactttc ttagacttta ctgatgaaat
      241 ggtaaaatat tcaaatgttt ggaaaaattt aaaagtcaat tttgatagta tatatagtgg
      301 ttttttgggt tcaaagcatc aaattgagat agtggctaat tttataaatg attatcccaa
      361 tgcttttatc gtagttgacc ctgtgatggg tgataatgga gttatgtatc caatattcac
      421 agaagaaatg agacaggaaa ttaaagaatt agttaaacat tcagatttaa ctactcctaa
      481 tttaacagaa gcttgtttct taactggaaa tgattatact aaaagcgatt ataatagaga
      541 tgagcttata tatattgcaa aatctgtatc tgatttggga cctagcaaag tcgttattac
      601 tggtatatta gaagatgata atatattaaa tttggcttat gatagagata atgaccatgt
      661 attctttaca tcagtcaaat acaataattg ttcttacagt ggaactggag atatattcac
      721 atctatactt tgtggtatgt tagtaaataa acatgatttg ggagtagcgg ttaatactgc
      781 aacagatttt atatataaaa ctataaatta cacatctcaa tttgatactg atagaaatga
      841 tggagttatg tttgaaaact ttttatcaga tttaactaat atataaaatc aaaaaaacta
      901 tccattttat aaatacctat aaatcataaa atttaggtat ttacaaaatg gatattattt
      961 actaaactat tggtatttca attgttacca tagcaccacc agttttcaca gaattttcaa
     1021 tatataaaat tccattatgt ttttgtacaa tattatttac aatataaagt cccattccgt
     1081 aatgagattt agaatttcta ctcaaatctc ctgtataaaa ctgttctgta gcttttgaca
     1141 aatctgcttt agaaaaacca cttccacaat ctgtaactac aaattttaag tatttattac
     1201 ttactgatac acttatgtat agtttactct ggcttggtga ataatctata gcatttgaaa
     1261 ttatattcat aatagctctg aataacagtt ctctatctat agttatattt ttaggcaaat
     1321 tatcttgttt ataaaactcc acatctaagt ttttagtact agctaatgct tctatttgca
     1381 gtaagacatc ttttataaac tcactcatat taattgcttc tttatttaat atatagccat
     1441 tctctgtttt tgatatatct attaaagtat taatatattt ttctatttga gtgacaccct
     1501 ttgagatata ttccatgtat tctaataact caggattttc atttatttca attaataagt
     1561 cagtatttcc atgtataatt gttaatggtg tttttatatc atgtgccagt gcagatatct
     1621 gttcctttct attttcttct agcatccatt gtttttctaa agaattcttt aacgccaact
     1681 tcattttatc catagaaaaa aggatattgt taatttcata aattttacta gattctacaa
     1741 caaaatctaa atcctgttgt tctatttttt ctgtagtatt ttttaaaagc tccatttcaa
     1801 tattaaattt cttgccaaat acttttgata atataacaat ttcaataaaa aatattatac
     1861 taaaaataat catacctaac atctctggtt ttggtaaata tgctcttaaa actggagaac
     1921 tatactctga tactagtttg tattttatta taaaaacttc atctttctta aaaaacttta
     1981 tataactttc tgaatatgca tgcctttctt caatatctct cattaaattc catacttgtt
     2041 ttgattcttt tttattaaaa gtgcctgaga tcaaattccc atctaaagta taaacaccat
     2101 actcgtataa atttggaatt atatcttcaa ttgctttttc acttgaaata atcttttctt
     2161 tatatttatc aaattgtttt tctgaataat tagctggtaa tattacacct gatgataata
     2221 atacagaaaa tgataacatt aaaagaagtg caaatgaaat tgtcataaca aagaataaag
     2281 ataaatattt tataaaaata gttcttaact tttcaacttt tttgttgtta gattttttac
     2341 ttctaagcat ttgtgccata tcctccaaat attttaatta attcataatt aatacctaac
     2401 cagtttttat ttgattattt tatatttttc attgctaagt tatattttaa cataataata
     2461 taaatataat gtaaataaac agtcaatttt tatttattct tctgactttt taccttccca
     2521 tttagaaaac caaatacata aaattacaaa tgctaaaatt gttacaaaac cacacattaa
     2581 acctgttgat tgatatgctt ttattgattc aacctttata tattcaaccg ttttagaact
     2641 tgaaaaagac gaccaaatag ttataaatct aacaccccat gcacaaggaa aatactgcca
     2701 aatgccagaa cccaatccag ttaataataa tgctgaaagt aacatttcaa ctatacctac
     2761 tccaattgat gttccttttc cgaaccttaa acttaagaat aaatgtaata tgtattcaaa
     2821 taaattacac ccaattaaaa ttagaattga aatcatataa aagcttgatg taaagtaagc
     2881 cttagatata ccaaatttaa aacctaatac agacactaat gttgctacaa aaccaagaca
     2941 tactaagatt atatatttac ttataagagt aagatatttt ggattagatg ttgttaacat
     3001 atgtttataa ttccctgcat aatattcttg ttcaacaaac attgaacaca caacacctga
     3061 caataaagga aaagctattg ctataacttg taaatatgct actatcttta atatattatc
     3121 agctcctgaa accttataat accataaaaa caatcccatc cccattaaag caatgcaaat
     3181 gtgcattagt attataaaat ttctctttaa ttttattaaa tcagaacaaa atattcttat
     3241 tagttgtctc atattacttc acctctctat tattgaacca agctcttgta actaaagcaa
     3301 ataatacaaa taaaataaca gatattaata ctcctggtaa aataacagca ttatttaaaa
     3361 gttcactccc ctctggtaca ggaagcccat ttggtagaat atgcaataca acaaccatta
     3421 atcttgaagg aatcgaatat ggaaatacat accacatttc tgtagttgag aaaacagcaa
     3481 ctccaacaat agtaaagaac atatttgtta gaactgtaac aaatatacca aattttgaag
     3541 ctaaaaacat acaaagagga atctgccaca taaatagtaa aataattaat atagttgcaa
     3601 tgatattttg acctattgaa atagtttctt tatagattat cccaacaaaa actacaccta
     3661 ttgcagaaat aatcgatgct atagaacaac aaataataca gtaaatcatt tttccagccc
     3721 aaacgtcttt catttcaatt ggcaatggta aaataccacg atatttactt ttttcatctt
     3781 ttaatactat taaagaagat attaatgtta atgctcctgg aagaaatgtc gtataccacc
     3841 agttgaaagc agcattttgc cctccaccta gtccacacaa aagtatacaa aacactggaa
     3901 ctatccatat caacttcatt ccaaaagttc ctttcatctt ttgaaattcc actctaataa
     3961 atctttccat attactcccc tctctttcta ttttcagcta caactttcat aaaaagtttt
     4021 tctaagtttt gttcttttga tacttctcct tgataaccaa ttactccatt agaaattatt
     4081 ccgatttcat ctacgacttg ttcaacttca gacaagatat ggcttgataa aataactgta
     4141 attccttgtt ttggaaaaga acgaatcaat tttctaagtt cttcaatacc aatagggtca
     4201 agaccatttg taggctcgtc taagattaaa agttttgggt gatttaataa tgcaatagca
     4261 ataccaagac gttgcttcat acctagagag aattgacctg aacgcttttt aaaagtatct
     4321 cttaagtcga cagtctctaa aacttcttca attcgagagt caggaagacc aagtaaagta
     4381 gtatggactt ttaaattttc tcgtgctgtt aaattctcat atatagcagg agactcaata
     4441 agagaaccaa tatttgataa atcctttcta gtccatctat gtccctcaaa cattattttt
     4501 cctgaagttg gatttaaaat cccagttatc atttttaata aagtagattt cccagctcca
     4561 tttggtccta ataatccata tatagaatta gatttaattg ctagtgaaat attatcaact
     4621 gccatctgct ccttaaattt tttagaaact ccttttacct gcaaaatata attattcata
     4681 attttcatcc tttcttttgt caaacttatt tacaagaatc attctaaatc ataaatataa
     4741 ggattttata agaattaaaa aacaaaaaaa agatgtgtta aaaacacatc tttttttatc
     4801 ttaattctat taattttcca ttgtagtata tctctacttc ttcaatccaa atgctattat
     4861 cttcttcttg gatgacattt ttaataatat tatttatagt atatattata ttttgttcat
     4921 tcaaataatc atctttccaa gaaactctac tttttttatc tggttctatc aagtttattt
     4981 taacaagatt atctttttcc ttaacttcta cttccatgtc taaaccatta aaatactcat
     5041 ttgataatgt atctaagatt aatcgcagtt tatccttaag tgaactttct tcatcaatct
     5101 gtaagtactt tactattttc tcatcctttg aaattatctc tataggtatt ttagtagttt
     5161 ttttagaatt aatcatttta ctagttgttt cttcttttgc atcttcatcc ttctttacaa
     5221 ctgctgattc tttagaatca actagctcaa ctgcactgat gctataatcc atacatgctg
     5281 tactcaataa agtagtagca attattaaaa aacttactgc tattttattc ataagtatcc
     5341 ctctcctaat tctctttcga tacttattat accatagtat agattatatt tttatacttt
     5401 taaatcaatt tataaattat tatagtaaat tatttacaat tctaagctat ttgaatttat
     5461 ttttatagta tattctttct ctaaattcga cagattttta ttcaaaagcc cttttatttc
     5521 cctcgtattt ctatacttgt tgctatgttc ttcactatgt tcataatcat atgaatcatt
     5581 gtcatattct tcatcattat taaattcttc aattgcttct tttgacttta tatcacaatt
     5641 aaatttatac ttatttactg gtatatcaag tcttacctta gaatttggaa cttctatgtc
     5701 caggaactta gcaattggca tattagagta caagtaaaat tcactttcat aatttgaatt
     5761 tctacctatt ttctctttta tatttacatt ttcaggtata tactcttcta tattattgat
     5821 aaatacatct tcattctctg aagatatatc caatgactct gattctatgt ttacattttt
     5881 aacgcctaaa attgattttg ttgacaagtg aagattgcta ttagatatta agtttaggtt
     5941 ttctaatcta tttactttgt tctctgttat agctctacta aaacctccat aagaagtttt
     6001 atatgttata gtatttgaag ctacattatc atatacaaat aagtatgaac ttgtacttga
     6061 tgcatttatg tctacattat ttggtacata tattataatt tctctataat catgtgaaaa
     6121 catactattt atagatgtat ttaataattg gtcgaaattt ttaatctttt taattttatt
     6181 ttcatatttt ctttcacctt ttacaactaa agtcttatct tttaaagtta cattgtattt
     6241 gtaaaactca ttatttcctc ttgttgttac acgcacatct tcacttgaac ttttctttat
     6301 tgtaacgttt atattgtcaa cagatatatc taacttgtca atatttactt tttcttgata
     6361 aagtttattt tcttttttaa attcagcttc cttttctaaa ccaaagttta ttaggcttgg
     6421 cataactaaa atccctgacc atgttgtccc tattatacct attaccaata aaactgcagc
     6481 aaatatagca agttttttat tcatgtttat cttctccttt cttattttct atatcttcaa
     6541 ggtccttata tatcgcctca aaatttatac tatcatcatt atattcatca gtttctttta
     6601 tgctttcttc ttctctattg tagtaatcat tattatatga atcttcatta atgtctttat
     6661 tcaaatcttc atcatttaaa gtagcttttt ttcttttttc catcttttca tttgccttta
     6721 tataaatatt ttttctcttt atccaatgta tataattatt tagtaattgt ttacctagtt
     6781 ttatagcaaa taataaaatt tgccatgcaa gtatctcaag ccctacaaat gctatggttg
     6841 cgaaaataca aaacatcata acatctggaa ccattgctat aaatttaatc acaaatggaa
     6901 ttaatataac aaaagcaatt aatgaacata ctaatgcaat agtaacagat acaatagcac
     6961 atacaaacgc aaatgctgga acaattagaa ataaagataa cactactaat aatcccttta
     7021 taacttgtct agataatccc cctacacctt cttctatact tggtgtaagt ttatcattta
     7081 tattttcttt tgatttattg tacttttcct tagcttcatc tttcaagata gagtaatcct
     7141 tttttatatt tgatttaaac ctactaaatt taccatttaa tatatctgta ttcattattt
     7201 ttccatctat attttctttt gatttactat acttttcttt tgcttcatct tttaagatag
     7261 agtaaccttt tttcatatct gatttaaatt tactaaactt atcattcaaa acatctgtat
     7321 ttattatttt ttcctcttca atattttttt ctttagtctg caagataagt tctttggcta
     7381 tagattttgg tgaaccaagt gatgctatta tctccatatc actctttccc tctataagac
     7441 catctacgaa atactcttca taatctctca atatatcatt aacttcgctc tcagaaaaat
     7501 ctttttttag ataatccttt aaaatttcaa gaaactcagt tttattcaag ttcattacct
     7561 ccctcatctt cacttattaa aatctcaaca gcctttacaa attctctcca ctcttccatc
     7621 agattggata aattctcttt tcctagtgaa gttattctat agtattttct agcaggacct
     7681 tcattagatt ctagtatata tgtttcgaaa tacaaatctt ttgtaagtct tcttagaata
     7741 ggatatattg ttccttcatt tacatctatt actttagaga tgttatgtac tatctcatat
     7801 ccatacatat ctttcttaga tatgagagca agtacacaaa tttctaaaac accctttcta
     7861 aattgtgtgt tcatatactc catcctttct ttaaatacac agtattgtgt tttacaaggt
     7921 actatgttaa aacttcttat atcctcataa taacacagta ctatgttctg cacaatagtt
     7981 atttttaaat attcttgttg attaaatcct tctataaatg tataatactc taatcagata
     8041 tattttatga aagtggtgat aaattggcta aaatgaaaga tgataaaaca atattaatct
     8101 taactgctca atttggtgct ggacatataa gtgctgcaaa agcagtcaaa gaatgtatta
     8161 ttgaaaaata tagtaactat aatgttgtta tacaaaactt tataaatgca agtattccca
     8221 tgatgaataa acctatggta aaactttatg aaaacaatac aaaatatact cctggattat
     8281 ataattacta ttattacttt aagaaatcgt ttgattcaag acatgattta tctcataaat
     8341 tatatacacc taaactttct gagtatattg ctgatataaa tccagatttg attatctcta
     8401 catttccact agcggctgct tgtgtaaata actttaagat aaaaaatcct gatataaata
     8461 tacctacact tacagttata acagatgttg tagatagtat ggaatgggtt tttgaaaata
     8521 cagatttgta ttttgttcct tctcctgaaa taaaaaatag attttttcag aaaggaataa
     8581 atccagactc cataaaggtt actggagttc ctgttgataa gagatttcaa attgaaagta
     8641 aagaactatg ttgtgataaa tatagactat tacttctagg tggaggaaga ggcctatttg
     8701 atatagatga ggattttatg cattggatag atgaatttat tgaagaacat agtaattcta
     8761 tagaaattac aatagttact ggtaaaaaca aaaaattata tgacaatcta actcataaaa
     8821 aacctctaaa aaatattaaa gtacttggat ttgtaaacga catgtataat ctaataagag
     8881 aatgtgacct aatgctaact aagcctggag gagcaactat atttgaagct attcaatcac
     8941 aaactccagt actcgtgaaa atgccaaaag ttggacaaga aattgaaaac gctaaattta
     9001 taatagataa gggtcttggc atgatttata gtgatgacct agaccttaaa aacatatttt
     9061 ataaattagt atcaaatgaa tttgattcta taataaactt tatgaaaaaa aatttagaag
     9121 aatttaaaac agttattcat ccagagaaaa ttgctgatta tatatcagaa ttaatagata
     9181 atcattacag ttaaattgta ctattaaaaa atttcaaaca taaaattaaa ggcaatcaag
     9241 tatgctttta cacttgagaa tttactcata gtgtttaata atacttgatt gcctttatca
     9301 tatcaaaatc aaattgttat attagtatat atttttcttg tactgcatat acagttgttg
     9361 tatttcttcc atacttctct ttaattctat ttgtaaatct gaagcagtct ggtaatcctt
     9421 attttctata gcttctctca ctctagtgaa tagtgattct ctagttaaag tatccttcat
     9481 aatattgaac tttaaatccg atattgagtt ccaaactacc tcatcaagcg catccatatt
     9541 ttcttttgtg tgtatcttga caatacttct tattttttga atcccagcct ctattattct
     9601 agtttttaat tttttttgcc acttatctat agctccaatc ttaaatgatt ctattatact
     9661 atcagtaaat acatcacctt gttttaaaga ttttaatttt gaagcatata tttctaagtt
     9721 tttcatattt tcataaactg tagccggtgg tattccaaat cttgcatttc tttcttccaa
     9781 actataatgt tcaaatacat cattttcatc tctatatgct ctatcttttt ctaaatagaa
     9841 actttcttca cctacatttt tagataactc tttttcaagt tcttttgtac taagaccact
     9901 cttagctgct gcttttatac catccaacat tgtttgataa catcctgcta ttactaaata
     9961 cgtgttagat agagggcttg gtgaccttaa ctcaaatctt acagtctttg gatttttcat
    10021 atctcttatt aatccaacaa gcactgacct atttcttgat ggaatctcat aactatgccc
    10081 taaagatgta actatgcata caggagcttc aaatccagga actaatctat taaatccatc
    10141 attagaagct gttacaattg ggtttaatac ttcataattg taaagaagtc ccataagagc
    10201 cccatatcct agttcactta gataatcttc ttttaaatct tttggagcaa atagattttt
    10261 tatacttcca tcctttaact tcgcacttac tcctacatgt gtatgtccac cacttcctgc
    10321 tactccatga attggctttg ctttaaatgt aacttctagt ccatgtgatg taaatacatc
    10381 ctctataaca tctcttacta ataattcatt gtctgctgct tgtaatggtg tagaaaactt
    10441 ccaagaaacc tctaattgct ccatagcatg gtttgttctt ccatctatac taatagaact
    10501 ttgtattcct ccgacctctt tgtgagccat ttcagggttt acacctagct tttgaagtat
    10561 aattaaactt ttctctaaac aagttcttat aataccatga gttcttttcc agtattgctc
    10621 ttttaggctt tgtgatacat ataatttctc taagtcagct ttatcctcag gagtattaac
    10681 ccagaactca agttctgtag ctgctgttaa cattatttcc tctatatcat ctactgaatc
    10741 tattcctatg ttatttatta catgtggata ttctctaaat atttcataca ttgacttttt
    10801 aaagtactta tctgcttttt gaagtactcc tctagaacat acttttttat tatcatgtat
    10861 taaaaatgca ggtatcttta atgttccaac aggtagacct acttcttcgt ctatatattc
    10921 catattgtaa tctacatacc aatgacaact tttatctggc attaaatcta ctttcgcatt
    10981 atttaatgta gctatattat aaagctcaac acttgaacca tcagtctgta tagctgattc
    11041 caaaaattta tctatatcct ccaaaaataa ttctacagga atcttttcat ctgttgcatt
    11101 tcctcctaaa tcaaccccca ttaaggaaac aaatcttata tttttattgc tttttaggat
    11161 ttctcttaat tcttcattag tgtgtttatc tttctctatt acgtataata gactatccat
    11221 ttttccactc tcccataatt tttatgtata ttttattttg ttaaatacaa gataaatttc
    11281 attttcctta cattcatgaa tttttcttgc aaattattcg tgttttattc gtgtttttat
    11341 ttatattatt atatatcaag tataaaaagt tcgcaatatt ttttattgga aaaaatatta
    11401 tacatacaat agtttaacat ttttacaaat atggggtata ctaaaataaa agtattttta
    11461 tttgaaaatt taattttatt tataatataa tctatagtaa aagaaataat aaaaaaattt
    11521 aggagataaa ctttatggaa tacataattt tcgatttgga atttaaccaa ggatttgata
    11581 aaaaactcaa taaaactgta tccaatgaaa agtgtccttt tgaaattata caaataggag
    11641 caattaaatt ggattctaaa ttcaatataa tagatacgtt taatagctat gtaaaaccta
    11701 caatttataa agacatacat ccctttatta gtcgtatgac taatattaaa aattctgatt
    11761 ttaatgattc gcctactttc cctgaggtct acaacaattt tataaaattt atatcatcac
    11821 aagaccctat attatgtgtt tggggagttg gagatttaaa ggaattatac aggaatatca
    11881 actatcacaa gctaccatca agttccttac ctaaatctta tattaatata caacaacatg
    11941 catctaaata tttcaataat ccagcaggaa aaagcattgg cctacaaaat gctatttcta
    12001 tattggaact tgatgaaaaa atgtcttatc acaatgcttt aaatgatgca tattatactg
    12061 ctaaagtttt tattaagata tacaatccaa gtatagtccc agatatatat ctttatacga
    12121 gtataaaacc taaaactata agatattcta ataaaaaaag agttgattat gataagttat
    12181 ttgatgaatt taggaaaatt ttaaatcgtg aattaactaa agacgagaaa aaaatcatta
    12241 acttagctta taatatgggg aaaacaaatc aatttacatt agaaaatgtt aagcaaagaa
    12301 aaaacaaata gagtttattt agataaaaaa taggcatgta gattaaaaaa ctagttaact
    12361 ttaaatttat atctacagcc caatttttat cgtaattaat ttttatttta tctctaattc
    12421 aactggacaa tggtctgaac ctaatatttc agtgtgtata tccgcactta ctaatctatc
    12481 ttctaatttt ttggatgcac aaaaataatc tattctccac cctgcattat tttttcttgc
    12541 attaaatcta tatgaccacc aactataaac gccttcttta tcaggattaa aatatctata
    12601 tgtgtctata aacccactgt ctaataattt agaaaacttc tctctttctt ctttagtaaa
    12661 acctgcattt ttcatattat ttttaggatt ttttaaatct atttctttat gtgcaacgtt
    12721 catatctcca cacacaatta caggcttatg attatctagt ttggttaaat aatctataaa
    12781 gtcgtcttcc catctagttc tatattctag tctctttaat tcagattgag aatttggcgt
    12841 atatactgta acaaagtaaa aatcttcaaa ctctagagtt atcactctac cctctttgtc
    12901 atgttcttct atatttattc catacattac ttttaaaggc tctttttttg taaatatagc
    12961 agtacctgaa taaccctttc tctcagcata attccaatat tgaaaatatc ctggtaaatc
    13021 taactctatt tgcccttctt gcaatttagt ttcttgtaaa caaaatatat ctgcatctac
    13081 ttctttgaaa aagtctaaaa atcctttccc aacacaagct cttattccat taacattcca
    13141 tgatataaat ttcatactta tactcctcat ctaaacattg acacactccc catagctaaa
    13201 gatgggggct tttcaacaat tttattgtaa attcttaatt tacttttatt ttaacaatac
    13261 tatactataa tttcatctat aattactatt tacttaatac ctaaaaatat atgttttaat
    13321 caatttttaa cttttttgtt aaatcttcag ggctcattga agaaacatac atatttgttc
    13381 caagttcaaa tcctccaaaa ttatattttc ttgggattat gtgcatatga acattaaaat
    13441 acgattttgt ttcaatcttt gttggatgag tgtgaataca taagttaaat ggcatatatc
    13501 cattgatatt gtataacttc ttaaacaact tttcaaatat tgtagaaagc tcctgtatat
    13561 tattatcatt taactcacca aactctattt tatctttaaa tattattctt acttctccac
    13621 tatacttggt tgcatatggt ataagtacca aaaaagcttc tccattataa acaactcttc
    13681 tatctaaact tatttcattt tctattaaat catcatacaa agagctttta ttgctgtcat
    13741 aatactcctt tgaaactaac agttcattcg taatttcagg tggaataaaa gacatagaca
    13801 atatttgagc atgtggatgc atcaaagatg ctcctgcctt tctcaagaag tttttgaaga
    13861 tactaacata ctctacttta tcatctttcg acaaatctct aaatctattt ctatacataa
    13921 aaaaaacatc ttcaaattct ttttgactca tgttataaaa actacgatta tgtttatcgc
    13981 tctctatcat tacttcatga acaccatata catctcttgc catatcatca attataggaa
    14041 atttattgta gacagatttt acaagccatc cttcttcact ttctatttca aataatgcat
    14101 cagttgcatg agcttcattt cctttgcaaa atgggcattc ttcatcatat tcattagttt
    14161 cttcatcttc atttgggata tctgctgtat ctaaaggtct ttttaaccta tctgtagcaa
    14221 atataacaac atcattagta attggatcta ttcttaattc tttcataatt aacacctctt
    14281 agttaatagc ctttaatata attatatgat atttatcgta tctgttaaat aaatttataa
    14341 tattttttat aattttgcat atttattatt atatattttg tctatagctg aaagattata
    14401 actaaaaaat cactatcagt gtatcagaat attatcttac tataaattaa tttatgaata
    14461 cataatttag tggcataaac atgggtattt tactataatt tgttaaaagt ttattttaaa
    14521 gctatattaa ttttttttga tgatttttac ccataagtat gttattatta tcttgtaaag
    14581 atgtcttgta ttcaaacata caatttacat aatacaatat acgtatttaa attaaatagg
    14641 gggtattaaa atgaattatt caaacagagt tagtgcaatg caagcctctc caatcagaaa
    14701 acttgttcca tttgcacaag cagcaaaaga taaaggaatc aaagtatatc acttaaacat
    14761 aggacaacca gacataaaaa ctcctaaagg attttttgat gctgttaaaa actttgatag
    14821 cgaagtctta gaatatgcta catcagaagg tattccagaa ttattagaag cacttcaaaa
    14881 ttactataaa acttacgata tgaattttga aaaagatgaa ttattagtaa ctaatggtgg
    14941 tagtgaagct ttacttttca ctatgatggc agtttgtgac ccaggagata atctattagt
    15001 tccagaacca ttctatacaa actacaatgg atttggtcaa tctgtaaatg tagaagttaa
    15061 tgctgttaca acaaaggcag aaaacggttt ccaccttcca tctaaagaag aaatattgtc
    15121 taaagtagat gataaaacta aggctataat tctttctaac ccaggaaatc caactggtgc
    15181 aatttataca aaagaagaac ttaatatcct agctgaaata gctaaagaaa aagatttatg
    15241 gataatagca gacgaagttt atagagaatt tgtttatgat gggcttgagt atacaagctg
    15301 tggtaactta gagggtgtac aggatagagt tataataata gacagtgttt ctaagagata
    15361 cagtgcttgt ggtgctagaa ttggttctat cgcttgcaaa aataaaggtc ttatcgctca
    15421 aatacttaaa ttatgtcaag gaagactttg tgttcctaca ttagagcaaa taggagctgt
    15481 tgagttatac aaaactcctg tttcttactt caaagaagtt aatgaagagt acaaaaaaag
    15541 aagagatgta ttatacaatg aattaatgaa ggttgaaggt gttatatgta aaaaaccaac
    15601 tggtgctttc tatatagttg ctaaattacc agttgaaaat gcagaagatt tcacaatttg
    15661 gatgttaaaa gaatttaaca aagataatga aacagttatg gtttgccctg ctgaaggatt
    15721 ctatgctact ccaggtttag gacgtgatga aataagatta gcttacatct taaatgaaaa
    15781 agaccttcat agagctgcta cattattaaa agaaggttta gagcaatatg tagctttaca
    15841 aaaataattt tttaaactta atttatataa atttaatgat ataaaatgtc cccttttcag
    15901 atattatttg aaaaggggac atttcttaat taatatatta attaagactt agacctgact
    15961 atttttcatt ttctttatat agtgttggcc agtaatcttt attcacttta attaaagcat
    16021 ctaaaacttg tcttgcctta gttggagaga ttatagttct atttaatgtt aaagataaaa
    16081 tagctttttc ataagattgt tcaaagtatg catcaactgc taacttttca taagctagtt
    16141 gaccttctaa cagtccctta taaaatgttg gtatattacc tacatgtaaa ggtctcggtc
    16201 cattctttgt aagaatcgca gctacttcta ccattgcatc atcaggtaag ttgttaatag
    16261 ccccattatt ttctgttata actatggcta tttggtgttt gttatgagct attgcttctg
    16321 caacttcaac tataaattcc ccatgagcat cattatgcac taaattagaa cctatagatg
    16381 ttcctttttc tataacagat ttacattctt caaatacacg cttttctctt ccattcataa
    16441 cttctcttgc tctagtacaa tttatatttg atttagctac ttttttatca ggatatagat
    16501 aatactgtac atatgtatta ggtaagtaat ctggtacatc ttccaacata tctttaacca
    16561 ttccataagt gtctaaccaa gatttatctc tttgttctgc atcaacaggt tggaatccag
    16621 agtttgctac taaatctttt atcttaggta ccaaatcttc accattttta tcatacaaat
    16681 gtgtaaacca tccaaagtga tttaatccaa agtatacagg ctcaaatgtt cttgaatcca
    16741 tgtctaacaa tcttccataa gaccttaaca agttaactgg ttggtcacac atattaagta
    16801 atcttttatc atctggaaat actcttttta atgcttctgc aacaatagca gcaggatttg
    16861 tataatttaa tatccatgct ttaggagagt attttcttac agtcttaacc atttctatca
    16921 tatcacctat agaccttaac ccataagcaa atccaccagc tccacaagtt tcttgaccaa
    16981 ccagtccaaa ttgtagagga atcttttcat ctaattctcg catcttaaga ccaccagttc
    17041 tcatttgaac aaatacaaaa tctacatcta taaatgcttc atctgggttt gtagtatatt
    17101 caagtgttgc tttaggatat tcttctttaa ataatatttt tccaaattcc cctattaatc
    17161 tttgacgttc ttctactaca tctaacatag ttactttatt tagaggaaat ctatcactca
    17221 ttttacataa agactttaaa agtcctggag tccaagtact gcctcctcca actatagcta
    17281 tattgtatct ttttttcatt gtcatattaa tcacccctgt tttttattaa ctaacttgta
    17341 agatgattat aatatataaa gttttctaca tgaatacttt taggtaatca ttaaaaatag
    17401 ttcaatttta gtattttttt caaaattgta atttttttca tctattgtat ttttcaatgt
    17461 atttatgaaa catatattcc ataagagaaa gtgctggcac tctagacgta tagttaacac
    17521 tattaaaatt atattgtgga gaagatacaa ataattttat atcagctata tttgataatg
    17581 aattgacact tatatttgtc agagaaatta ctttaacttg atttgttttt gctatattac
    17641 atgccttaat aacttgagca gtctctccag acatagatac agcaattaca aggtcatctt
    17701 tagtaatatt tgatgcagcc aaatacatga gagttgaatc atcatagtat tttacattta
    17761 cacctatagc tgaaaaacgt tgagacattt cagtacatac taatctagaa gacccaacac
    17821 caaaaaaata tatatgctta gaattatgta tttcatttaa acacatatct atattctcta
    17881 catttattaa attttgtgta tccataacat cagtaaaaat atcactttga cttttagtaa
    17941 actccttatt tctttcattt ctatttgaat atgaaaaaac taaaaagttt ttaaattcac
    18001 tatatccaga aaagccgagc ttctgacaaa accttactat gtatgcagtc gaattaaaag
    18061 tttcatttgc tatatcttta atcttttttt gatttatttt ttcagaattt tttagaaaat
    18121 agtttaaaag agatatttca tttgtagata attttttttg atttatatct aatttgtcta
    18181 ttaggttcat ttatacctcc ataataatga tttgtagtta aacaactttc tcatatttat
    18241 tatataaata tacactatac aacatttatc ttatgtttat tatactacaa aaaagacaat
    18301 acaaattata aattagtatt gccttttaat atttacatta gatagacatt aaatagacat
    18361 tgcaaaagaa acttcgtctc ttacttgaga tacagatggc ccataaatta tttggatatc
    18421 tttatcagat acttttataa cgcccattgc acctgtatat tttttaaaag cattttcatc
    18481 tactttactc atatctttaa ctactactct taagcgagtt atacagtttg ttatatcttc
    18541 tatattctcc ttaccaccta aatatttaat tatggataat gctctcttag tagtttctcc
    18601 actttgagct atttctactt tatttgataa aggttctaaa tcaaaattta tatctgttat
    18661 ttcttctcgt cctggagttt gtaagttaaa tctaagtatc aaaaccttaa atacattata
    18721 ataaaccagt gcatacacta aaccaagtat tatagaataa atccactttg tatttggttg
    18781 cataactcca aatactacta aatctattat gccaccttga gtatttccta ttgctactcc
    18841 aaatatatgc atcatcataa atgatatacc tgataaaaat gcatgtataa caaataatat
    18901 tggtgataca aataagaatg aaaattccaa tggttccgta attcctgtta caaatgttgc
    18961 taaagcacca gctagtaata tacctttaac cttcgaccta ttttcaggct ttgcagtttt
    19021 atacatggca tatgctgcag caggaagacc aaataccatt ataggtattt tcccttgagc
    19081 taaaaatcta gttgcttctg ttgaaaaact gattgtttca gggtcactta gttgagctaa
    19141 aaatatgttc aatgctccaa atacttcttg tcctcctaca actaaagaac ctcctacttc
    19201 agtaaatcta aacatagcat ttaatatatg atgtaatcca aatggtatta aaagcctttc
    19261 aagaaatcca aatacaaagg ttcctaaata tcctaaacta gctataccaa tacctacact
    19321 ctgaataatt gtatttacat atggccatat atatggtatt attatcccca aaatagagaa
    19381 tactactgtt gttattattg gaacaaacct agttccacca aagaacccta aataatctgg
    19441 cagttttata ttatagtatt tattatgtaa tgatgctgtt attataccaa ctacaattcc
    19501 accaaatact cctaactcta atgtttgtat gcctaaagtt acagcttgac ccatttctct
    19561 catcatgtca ggttcagcaa gaatacctct acaccctaac atataattta cactagtgtg
    19621 taatactaaa taaccaacaa aaccagaaaa tgcagaaact gctttttctt ctttagcaag
    19681 accaacagat attgatatag caaagaatat tgctaagttt ccaaaaagta ctcctgttat
    19741 agcttttgta aaacccaata ttccttgaat aaaattattt tctaaaaaag gtatatcact
    19801 aactatatta ggatttgaaa atgctgaact tattcctaaa aatatacctg cagcagatat
    19861 taatgctatt ggcaataaaa atgattttcc taactgtaaa aaaaactctt tcattttttg
    19921 tttcatttct atccctccat ttttaaattt ttaaaacgtt ttctatgtca taattatata
    19981 tttttatttt gtttttgaaa ctttataatt tgttttaaaa tatttttaca taaatagtaa
    20041 ttttttcaaa actgtaaatt ttttcaaaca aagacatagt aacattgtga tatatagaaa
    20101 tttaaaaaga gattagaaaa taaaattatt gaatataaaa tggaaaaata taaattacac
    20161 aaagaggtat gagtaggtac taacaaataa gaaaatgtat aagaaattat aaaaaagtac
    20221 atcaagaaaa tttaagatgt actttttaga ttgtatttag acttataatt atatagttaa
    20281 atgaataaca aagaattaat cttagtacta ttaaatataa aaatttttta tattttttct
    20341 atctaaattc ttctttgttt aatatatatt gaagagtatt tattgcttct aaatattttt
    20401 ttctgtcttc ttcagttaat ttagaaagtt tttctcctaa accatcactt aatttatcca
    20461 taaaatcagt tgctatctta tctccctctt cagtaatgtc aattagatat tttcttctat
    20521 caactaaatc tcttttcctt gttacatact tcttatcagt taaatcatca accatactag
    20581 ttaaactgcc tttttctata tttaacttag agcataaatc agtcatactt attactccat
    20641 aattttttat gaaaactaat gctcttaatt gtgttttatt tacattataa tctggtgcaa
    20701 atttttttag gtcttctaag tacagactag aataaatctt aggaaatgtt tttactaaaa
    20761 aacttatggt atcttttact atatagttca tataatcacc tcggttgttt taaaatttat
    20821 taaattatct aaataacttt atttcagttt acattaaaaa tattgcaagt acaatagttt
    20881 ttgctgaatg tttaaattta attagtaatc ttatcatttt tttattttta tttatattat
    20941 ttctattaaa tattatttat atagctattt tattaaattc atggtatagt aagtaaagaa
    21001 gaactcttca cagcgctgaa gagaagacgc tagcccatta aaatggctat cttatttata
    21061 aataagatag ccgtttttaa ttatatttta tctgtacaat tctatactct taggtccaca
    21121 tctacttttc ctaagtcttc cttataatct tttatagcag gttctacaac ttcatttaca
    21181 aattctacaa cttgttctgg agctctacct ataaagtttt ttgggtccat tatagatagt
    21241 atttcttcct tgctcatttt aaatgaatca tcattcatta taagttcaat taagttatta
    21301 tccttacctt cgtgctttac tctataggca gctttcatag aatattctct tattatttca
    21361 tgtaattcct gtctatctcc acctcttttt acagcttcca taagtattgt ttctgtagcc
    21421 ataaaaggaa gttcttcatt tacacgttta tttatcatat tttcatatac tactaaacca
    21481 tcagttacat ttatccctat ttcaagtata gcatctgctg ccataaatgc ttgaggtatt
    21541 gccaatctct tattggctga gtcatctaat gttctctcta accattgagt ggcttgtacc
    21601 attgcagggc ttatagattc agagattata tacttagata aagatgctat tctctcactt
    21661 ctcattgggt ttctcttata agccattgct gatgaaccta tttgattttt ttcaaatggc
    21721 tcttctaatt cttttaaact ttgtaaaagt cttatatcgt tagtcatctt atgcatactt
    21781 tgagctatcc ctgatagtat gcttattact tgataatcta actttctagt atatgtctgt
    21841 ccacttactg cataggaaga atggtatccc atctttttac aaattttctt atctaactct
    21901 tttacttttt cgtggtctcc ttcaaataag cttaaaaaac ttgcttgagt accagtagta
    21961 cctttaactc ctcttaactt catgttatct attctatagt ttaaatcttc caaatcaaga
    22021 attaaatctt gagcccataa acaagctctt tttcctactg ttgtaagttg agcagcttgg
    22081 aagtgtgtaa atccaagagc tggcatatct ttgtacttaa gtgcaaattc ttttaaattg
    22141 tttattaaat ttacaagctt tactcttaga agttttaaag cttcattcat ttgtataaca
    22201 tctgtattat ctccaacata tgcagatgtt gctcctaaat gtattattcc tttggctttt
    22261 tcacattgta aaccataagc ttttacgtga gacattacat catgtcttac ttctttttct
    22321 atttttctag cttcatcaaa atttatatta tctatattag ctcttagctc atctaattgt
    22381 tcttcagtta tattgattcc taactcatgt tcaccttctg caagtgcaac ccataacctt
    22441 ctccatgtag agaatttaaa ttgtggagaa aatatataac tcatgtcctt gctacaatat
    22501 ctgtctgtaa gtgggtttga gtaagtagta tatttattat ctatcattat attagcacct
    22561 tccttaaatc ttttgtcgtg tgattactgt gtacacatta gtacataaat aattatatat
    22621 aattttacga actataacaa gactttttta ttttttgttc acatctatat atagtatttt
    22681 ttatttatta ctatttttta tttatatcca aattatcttt ttcaattaag taatccacta
    22741 tttcactatc aacaaaattt aatacatact tatatctaat tgcatagtat ttatcactcc
    22801 atccaagctg attttttaca tcactttcat ctgccccaag ccttaaactt attgctgcaa
    22861 atgaatgtct aaaatctctt gctgaatatt gagaaagtcc agctaaatct aatgcttttt
    22921 tgactataag tctgacattt ctatctgtta tagaattact cttttgagtt gtaaaaataa
    22981 aatcatcact aggtattatt acctctggaa gcccagaata attcctgtaa tcttctaaaa
    23041 gtttaaaaaa gtatggatgt aatttaacta ctctttcttt tcttccctta cctaacctta
    23101 catatgagtt atcgttttca tctaccataa ggtctttcca ctttaagcta actaattcat
    23161 ttaataaaca cccagtagta actagacaga ctattattac tctatctctt atatttaatt
    23221 ttggtaggat acctataagt ttatttattt cttgtatact tagaacatca tcttttgttt
    23281 ttattcttgt tactgtgggt ttttctacat atctaaatgg atttatatct atgtatcctt
    23341 ctttttttaa gaagttataa aaactatgta aataactata tatcttttca caagttgatt
    23401 tcgcatattt agtctgtata tagtctacaa aatttttaca atcttctttt gtagcataaa
    23461 tcaattcttt cccttttaaa aattctttaa acagtataac ctttgataaa taatcgtgtt
    23521 tcgtgtttgg atttaatttt gtagaaaaaa cattaaaagt ttcatactca taatcatcta
    23581 gtatttttgt actatacata ataactcctt tccttttcat aaaataaaat agctatactt
    23641 ttactatacc atatatttcc ccctcaaata taccccttat tcaatagata acttttaagt
    23701 ttattttgtt atattatgaa ggttttatga aaatatcaat cttccattgt gcactacaca
    23761 gtactatggt attataagtt agtaaaaggt ggcatttttt tattaatcta tgaataaaat
    23821 atgctactat aaatttgtat tggaggtatt ttgatttgga aacatttagt ttgcttgaaa
    23881 tttttaaggc agtcattttg ggcatagttc agggaataac agaatggcta ccagtaagta
    23941 gtacagggca tatgatatta attaatgagt ttataaaatt gaatttttca aatacattta
    24001 taaatacatt tttagttgta attcaatttg gttcaatact agctgtactt gttatatttt
    24061 ttagaaagtt aaatcctttt gatagtgcaa agaatattaa acagaaaaaa gaaacagttc
    24121 gtttatggct taaggttata attgcagtta taccatctgg agttattgga atactgtttg
    24181 aagatgacat agacagagta ttctttaatt caacagtagt tgctattgct cttattgtct
    24241 atggtataat aatgataggg cttgaaaaga gaaataaaag acctaagtat aaagattttt
    24301 ctcaagttac atataaatta gctctttgta taggattatt ccagtgtcta gctctcatac
    24361 caggtacatc taggtctggt tctactataa taggtgcagt attacttggt acttctagat
    24421 atgtagcagc agagttttca ttcttcttag ccattcctac aatgcttgga gctagtgcat
    24481 taaaacttct aaaagctgga tttggattta ctggatttga atggcttatt ttaggtgttg
    24541 gttctgtagt agcatttgtt gtttcaattg tagttattaa attttttatg gactatatca
    24601 aaaaacatga cttcaaagta tttggttatt atagaatagt cttgggtata gtggttcttg
    24661 cttatttctt tctattataa tgtattctaa tttaataaca aatgataagg agaaaagtta
    24721 tgagtgttaa aaaagaaata tttgattgga ttaagtcaat agctatggct attgtacttg
    24781 catttgtaat tctacaattt ataagacctt ctgttgtaag tggagaatca atgtatccta
    24841 ctttagatga taaagattat ctgattttaa ataggatatc atacaaggtt ggtaaacctg
    24901 aaaaaggcga tattgtagtt tttaaaacca atttagttga tggagaaaca ggaaagaaaa
    24961 aagacttaat aaaaagagtt atagctactg aaggtgacag aataaaaata tcaaattcta
    25021 aagtgtatgt aaatggaaaa ttattaaatg aaccatatat acacaataac tatacttctg
    25081 gagatataga tactgttgtt ccaaaaggta aactatttgc aatgggagat aatagagaaa
    25141 atagtaatga tagtagattc cctgatgtag gtatggttga tgaagatgaa attcttggta
    25201 aggttatggt gagactatta cctcttgata atattgggaa agtggactaa aaaataagag
    25261 actgaattgg taaagtttat tttacaaatc tcagtctctt tttattttct taaattacaa
    25321 atattttttt actaaatatc ttttgattct gtaataagtc ttattatatc acttgcttct
    25381 tcattaaaag ttgttactat acttaggtag tctgttgctc tactcatagc taaataaacc
    25441 ttatttaaat ctcctacaaa gtcatttact tgataatctt gttcaatatc atttatagtt
    25501 tgattgtata acatctctaa ttcacatact attaccgctt tatactctaa attctttatt
    25561 gcatatatat ttgctattgt agttcctacc ttcttactta tatttgtcaa attatcttct
    25621 gcacatatat aaggaatttt tgcctcttcc aatgcttttt ttaacatata ttgaaagtaa
    25681 atggtttttc cactttttaa tttcttctta ttataaggat aaacgatagc aacttcagaa
    25741 taatcaagac cttttttact tattaaatac tcaatttccc agattacaga acttatttgg
    25801 tcatcaaggt cacttacttt tattatatca acagacttat ttccacctct aagtgcttta
    25861 gtataaaaga aagtactaaa ttctgtattt ggtcttaatt cgttgatata tgagttagaa
    25921 ttatttgaaa atttatttgt aaaatctaca atctctttag cttgtctata atttttactt
    25981 aagataatct tatcatcaaa ttctatacca ttatataatt ttttaaatat attcaatcta
    26041 ttagagatat tcaaggagtt acaagaacac acattaaata tgaattttgt tttatataaa
    26101 aactccctta taaaatctat ttcatactct aaaaagcttt ctgcttcatc aataaaaata
    26161 gccttgaaca tatttttatt ttttataatg ttctgtgctt gttttacaag attattaaag
    26221 gtcttttcgt aatcattttt taacatgtta taatctacaa ttaaattgaa ttttttagct
    26281 agtttaaata taaaagaact aaatgtgtga acttctaaat tgttattatc tttgtataat
    26341 aactctatcc tctctcgaag ctcattgcac aattgttttg tatgtgtaaa tatgactaat
    26401 ttatgatgtg gatatacttt tgctaatttt atagccctag aaagcataat tgctgttttg
    26461 cctataccag aaccaccttc aataagtgta ttcccatatt taatagaaac aacatcttta
    26521 atttgaacgt cttccatcat agttgctgta tacttatagt catcattgta gaatgaaatt
    26581 tttttaaatt tttcatttag atgaagttta tcatttatta tatagtattc tgaacatatg
    26641 tctagtaaaa ataaatttaa gtttatttca tcattctcat cattaagata ctcctccagc
    26701 gaacccttat tcattatatc ttgaagctta tttttatcta ttatattatt attaacaaat
    26761 tcttcaaatt catatgtttc ctcaacttca acgtatggca taatgtaaac gtagttataa
    26821 cttatatttc tattcttttg tcccattttt agtttcaaaa gttcatattc ctcattcata
    26881 acttcaagta actcttcttc taaaataaaa aatatatctt ctgttgtatc cataaatttt
    26941 ataaataaaa ttcttccatc tttaacatac aacaaatcgg tatttattcc tttaaaagga
    27001 gtaacttttg gcactacttt aacgtcctca ttgatatttt cgtaaaatat tttctcgaac
    27061 tccttcaatt ttagggcgtc taactgagaa tttatattta tgtattggct cacttacatt
    27121 acctccttct gttatactta attataccat tttgaactat atttttatat atacttttta
    27181 taggcaaatt tagtaaataa attcaaaata aatacaaata aatacaaaac tgctaaaata
    27241 attttgc
//
LOCUS       DAELXX010000050        27011 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.050, whole genome shotgun sequence.
ACCESSION   DAELXX010000050 DAELXX010000000
VERSION     DAELXX010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27011
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.050"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            29..367
                     /locus_tag="KPH29_001971"
     CDS             29..367
                     /locus_tag="KPH29_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HBG1467566.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGRP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(494..1783)
                     /locus_tag="KPH29_001972"
     CDS             complement(494..1783)
                     /locus_tag="KPH29_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG1467567.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFFTGNNILMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
     gene            1863..2567
                     /locus_tag="KPH29_001973"
     CDS             1863..2567
                     /locus_tag="KPH29_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HBG1467568.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSLIFKDDIYVVSKKNLPNE
                     KTHVVENEIKGEDNNHNKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKPNQKNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            2659..3705
                     /gene="selD"
                     /locus_tag="KPH29_001974"
     CDS             2659..3705
                     /gene="selD"
                     /locus_tag="KPH29_001974"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2707..2709,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBG1467569.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDNPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            3723..5135
                     /locus_tag="KPH29_001975"
     CDS             3723..5135
                     /locus_tag="KPH29_001975"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBG1467570.1"
                     /translation="MIVLSKRELFAMLPSVDEVLSDNRIVEIINEYPRSLVLESVREV
                     IDLKRQFILRLKEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLG
                     RSLLSEDIKDELWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNA
                     AAVLLVLSTMAKGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYK
                     EAITEDTNVLMKVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKY
                     GLSYEPTVLDSIRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDK
                     LTICALEATLRMYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINI
                     EDGFSQVGGGSMPLETISTKVISITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDV
                     RTIFDDEFDVIVAELRKAFN"
     gene            5249..7132
                     /gene="selB"
                     /locus_tag="KPH29_001976"
     CDS             5249..7132
                     /gene="selB"
                     /locus_tag="KPH29_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBG1467571.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALEALISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            7237..9975
                     /locus_tag="KPH29_001977"
     CDS             7237..9975
                     /locus_tag="KPH29_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HBG1467572.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLVATGVIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSIILFIATATLTITSLFIISGICDYL
                     GNGKSQYALGVIISIFIIIISIISGIELNSSSNKGKWEYDEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVERILSQIIINVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESL
                     KEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNAEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSKDFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEYELINSYDDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRNEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVVSEVNKGTEFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(10094..11038)
                     /gene="manA"
                     /locus_tag="KPH29_001978"
     CDS             complement(10094..11038)
                     /gene="manA"
                     /locus_tag="KPH29_001978"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBG1467573.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            11211..12572
                     /locus_tag="KPH29_001979"
     CDS             11211..12572
                     /locus_tag="KPH29_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG1467574.1"
                     /translation="MELFKAESKKYEKEFLKLLEQWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSIVPTIINIVDGKLEVVLSVRYPEIL
                     TIEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            12660..14549
                     /locus_tag="KPH29_001980"
     CDS             12660..14549
                     /locus_tag="KPH29_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG1467575.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAVKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIYCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISSVISSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKASTK
                     EEVIISLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            14564..15019
                     /locus_tag="KPH29_001981"
     CDS             14564..15019
                     /locus_tag="KPH29_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG1467576.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNLINEALGL"
     gene            15078..15395
                     /locus_tag="KPH29_001982"
     CDS             15078..15395
                     /locus_tag="KPH29_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBG1467577.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            15420..16682
                     /locus_tag="KPH29_001983"
     CDS             15420..16682
                     /locus_tag="KPH29_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBG1467578.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFIDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            16787..17851
                     /locus_tag="KPH29_001984"
     CDS             16787..17851
                     /locus_tag="KPH29_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG1467579.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKIGMTE
                     KQVENEIVRIIKDLGGQKESFDTIVASGLRGALPHGKASEKIIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YANNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            17905..19083
                     /locus_tag="KPH29_001985"
     CDS             17905..19083
                     /locus_tag="KPH29_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HBG1467580.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFVPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGKFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKNIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
     gene            complement(19274..20332)
                     /locus_tag="KPH29_001986"
     CDS             complement(19274..20332)
                     /locus_tag="KPH29_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBG1467581.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTIFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSEIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLEDGKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKDGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMDLINEIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNKQ"
     gene            20399..20770
                     /locus_tag="KPH29_001987"
     CDS             20399..20770
                     /locus_tag="KPH29_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBG1467582.1"
                     /translation="MKKKDILLILGILVVIIACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIENRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            20807..21481
                     /locus_tag="KPH29_001988"
     CDS             20807..21481
                     /locus_tag="KPH29_001988"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBG1467583.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            21730..22569
                     /locus_tag="KPH29_001989"
     CDS             21730..22569
                     /locus_tag="KPH29_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBG1467584.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDANDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            22812..22908
                     /locus_tag="KPH29_001990"
     tRNA            22812..22908
                     /locus_tag="KPH29_001990"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22846..22848,aa:Sec,seq:tca)
     gene            23059..23766
                     /locus_tag="KPH29_001991"
     CDS             23059..23766
                     /locus_tag="KPH29_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HBG1467585.1"
                     /translation="MENNFTTRTSFLVGDDGIEKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
     gene            complement(23908..24744)
                     /locus_tag="KPH29_001992"
     CDS             complement(23908..24744)
                     /locus_tag="KPH29_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG1467586.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHIIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNVIKKFNVNSIY
                     MPEQSTDSEAYQNLINSCTDKNLSIQHLYKNDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNDNSFLFMGDAEEENEKEILHSFKLNNINFIKIGHHGSNSSSSLEFIKKIS
                     PDIAAISCGYKNQYGHPHREVINNLKQNHVSIYRTDRIGDIVFYSDGEIIFTKYNYEI
                     DT"
     gene            24932..25837
                     /locus_tag="KPH29_001993"
     CDS             24932..25837
                     /locus_tag="KPH29_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HBG1467587.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            25846..>27011
                     /locus_tag="KPH29_001994"
     CDS             25846..>27011
                     /locus_tag="KPH29_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG1467588.1"
                     /translation="MEIRYAKEEEIESIKEIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     RIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIEKDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNCIFSKKNT"
BASE COUNT        10761 a         2833 c         4594 g         8823 t
ORIGIN      
        1 caatatcaaa taaatgggag gaaatctaat gaacttatta gtgattggtg gtcatgaaag
       61 aatggaaaaa gattatatgg ttatggccaa gaaaaaaggt tataagacaa aggtatatac
      121 taccatgtca tctaaattaa ataattctat aggaagacca gatgcagttg tcatacttac
      181 atcaactgtt tcacataaga tgtctagaac ggttgagtct caagctaaaa aacaagatat
      241 attaatcgta aggcataaaa atagtagtaa ggtagcattt aatgaatgtc tagatatggt
      301 tgatgaatgt ctgggaaatt gttcaaaatg taagcttaaa gacaaacaaa aacaaaaaaa
      361 tatataggta taaaaaatac ataaacataa aattaaaagt actgagataa tagttataat
      421 tattatctca gtacttttag atttataagg ataagtatat tataaatttt ttattataat
      481 ttccaactac ttgttacttt ttcatatttt ttttacttct agcttttttt ctttttttct
      541 tctttatatt tgaagctatt ataaataaaa ctaaaagcac tagaatacct gcaataatct
      601 taacaaaagt cattaaaata ttatttccag taaaaaatgg aagactgcta tcaagattat
      661 tttttgcaat taagtctata ctgttttcta gtttaccatt attatataca tttaaagtac
      721 caaccttagc tccttttttt ataggcaaat caattttatt taactctgtt ttggtatcat
      781 aattgccttg agattcatct ttttctaaga atatcctata actatttttt ggttcataaa
      841 tcaattcatg ttgttttgta aacaatactt ttttagtatc tgtgtatttt tctttatcaa
      901 caatagtagc acacttaaag ttatcaaatc cataatcaat cagaatacgt gagtctaaat
      961 ataaatcagc attagttgaa ttaaatacag caacgataac tcttctgcca tcttttacag
     1021 cactcgataa aagacattta ccagcagcat cagtatatcc agtttttata ccatcaacta
     1081 tatcatattt tatatttata ggttgacctt tgtatgtaat cttatcatgt gatgtcaaaa
     1141 acttattagt gtttacaaag tatctctcat aaggatatgc ttttgtagct tcaaatttaa
     1201 gtgacttagt ttttacaatt tgtctaaaag tatcattatt catagcttct ctagctatga
     1261 gagccatatc atgagctgtt gtgtagtgat ttgggtctgg taatccatga ggattattaa
     1321 aatgagtatt agtagctcca attttttttg cctcagagtt catgaggtca ataaattttt
     1381 gtacattgcc accaccaaca tatctagcca atacaaaagc agcatcatta gcagaatgaa
     1441 ccaaaagagc atctagtaac tgctttacag taaaagattc cccatttttt agatacatac
     1501 tagagccatc tatttgaggt aaatctttaa tttctattac ttgatttaaa tctttaacct
     1561 cctttagaac taaacaagct gtccatactt tagttgttga agctggataa agcttagaat
     1621 ttccatcctt attatatagt attttgccag actcatagtc cattaaaact gcatgtttag
     1681 caactatagc tggttcatta tctgcaaaag agatattaga gcttcctaaa aatgaagtaa
     1741 atatcaataa acagattaat aaaagtgata aatttctttt cattgtgagc ctccaaattt
     1801 taaattcatc ttttagtata gcatatattt gtcaataatt aaaagaaagg agtgattaat
     1861 atatgaaaaa aaaggtaaaa tttatttcgt tttttgtttt atttatttta ataatatcat
     1921 caataatcgt ttataaatcg cttattttca aagatgatat ttatgttgta agtaaaaaaa
     1981 atttacctaa tgaaaaaaca catgttgtag agaatgaaat taaaggtgaa gataataatc
     2041 acaataaagt aactaaagac atagataaaa attttcaaaa taataaaata actatataca
     2101 taagtggagc agtaaataga cctggaattg taactataga gtcagacaag agactttatg
     2161 atgctgtaga attactagga ggaacgacaa aagaagcaga tttaaatgga gtcaatctat
     2221 cagtaagatt agaagatgaa caacattaca taatacctaa aataggtgaa gcaacatctg
     2281 ttacaagtaa tgatgattcc aataaaccaa atcagaagaa tgagtcaaga ggtgagccta
     2341 aaaataaatc caataaaaat atatctaacg aatcaaaagt aaatataaat gtagcgacta
     2401 tagaagagtt agattctcta cctggggttg gagaagctac agccaataaa attttacaac
     2461 acagagaaga aaatggacag tttagttcaa ttgaggaaat aaaaaatgta aatggtattg
     2521 gagataaaaa gtatgaaaat ataaaagatt taatttgtgt agactaaaat ataatacaaa
     2581 acttacatat agatatgtta ttataaatat gtatgtatat gtgtgtacat gtggaaattt
     2641 agtaaaggag gcacttttat gaaaaaagaa caaaaaaaac ttacagagat gacaacagca
     2701 ggtggttgag ccgctaaaat agggccagag gttctggcat cagttttatc tcaattgcct
     2761 aaaaatgata atatagaaaa tttattagta ggactagata cagcagatga tgctgcagtt
     2821 tataaattaa atgatgatat ggcacttatc caaactttag acttctttac accaatggta
     2881 gataacccat acgtatttgg tcaaattgct gcatcaaatt cactatcaga tgtatatgct
     2941 atgggaggca aacctttggt tgctatgaat attgtttgtt ttccatcatg ccatgatatg
     3001 gatgtacttg cggaaatatt aaaaggtggg tttgataaag tgaaagaaag tggagcactt
     3061 ttggttggag ggcatactgt agatgacaaa gagcctaaat atggtctttc agtttctggg
     3121 attgtaagtc caaataaagt tttatcaaat gctactgcaa aacctggtga taagttaatt
     3181 ataacaaaac caattggagt gggtgtatta aatacagcca tgaaagaagg tatggtagaa
     3241 caacatatag cagataaagt aatagaaata atgatacatt taaataaata tgcagcaatg
     3301 agttttgaga aatttgatgt aaattcggtt acagatataa ctggttttgg tctacttggt
     3361 catactttag aaatggccaa agcttctgaa gtaagtattg aaattgaatc aaaacatgta
     3421 ccaattattg aaggtgcaat agaaatggca caaatgggaa ttatacctgc tggtatgtat
     3481 aaaaatatgc attatgtatc taaagatgta gaagttgtag gaaatataga agttgcagtt
     3541 caagatattt tatacgaccc acaaacatca ggagggttgt taatttcagt aaaagaagaa
     3601 cttgcagagg aacttgtaaa agatatgaaa ttaaatggag caatagaagc taaaattata
     3661 ggttctgtag ttccaaaagg ggaaaaatat attacagttt tataaaatat tatagagaaa
     3721 gagtgattgt tttgagcaaa agagaattgt ttgcaatgtt gccttcagtt gatgaagtat
     3781 taagtgataa tagaattgta gaaataataa atgaatatcc tagaagttta gttttagaat
     3841 ctgtgagaga agtaatagat ttaaaaagac aatttatatt aagattaaaa gaagatgcat
     3901 caaactctgt aacaatagag tttggtgaaa taatagaatc tgcaatagag agagttaagt
     3961 taaattactc attatcttta aaaaaagtta taaatgcaac tggtacagtt attcatacaa
     4021 atttagggag gtctttactt agtgaagata taaaagatga attatggtgt gctgcttcaa
     4081 ggtattcaaa cttagaatac gatttggata atggagaaag aggttcaaga tactctcatt
     4141 taactagtac aataaaaaga cttactggag cagaagatgt actagttgta aataataatg
     4201 cagcggcagt acttcttgta cttagcacaa tggctaaagg aggagaagca attgtatctc
     4261 gtggagaatt agttgaagta ggtggttcat ttaggattcc aagtataatg gctcttagtg
     4321 gtgcagaatt agttgaagtt ggttcaacta ataaaactca tttgaaagat tacaaggagg
     4381 ccattacaga agatacaaat gtattgatga aggttcatac aagtaattat agaataatgg
     4441 gattcacaga gagtgtttct atagaagaat tggttaattt aggtaaaaaa tataaactac
     4501 ctgtaataga agacttagga agtggagttt ttatagattt atcaaaatac ggtttatctt
     4561 atgaaccaac tgtattggat tctattcgac aaggtgcaga tgtagtaact tttagtggag
     4621 ataagatgct tggaggtcca caagcaggta ttatagttgg gaaaaaagag tatatagaaa
     4681 aaatgaagaa aaatcaactt actagagcac ttagagttga taagcttaca atatgtgctc
     4741 ttgaagcaac attgagaatg tatttagatg agacaaaagc tatagaaaat atacctactt
     4801 taaaaatgtt gacatataaa attgaagaat tagaagtaaa agcaaataaa ctatttgaaa
     4861 aaataactgc tttaaactta aatgcaaaca taaatataga agatggattt tcacaagtgg
     4921 gaggagggtc tatgcctctt gaaactataa gtacaaaggt aatatcaata actcctgaac
     4981 atatgaatgt ttcatcttta gaaaaaaaac taagacttag tgaagcacat ataatagcca
     5041 gagtgtatga taataaatat gtactagatg taagaactat atttgatgat gaatttgatg
     5101 taatagttgc ggaattgaga aaagcattta attaaataaa tttgagctaa taaatattaa
     5161 atttagaagt ataagtatga agttttgatt aaaaaatagc tacacacact tgtgacttta
     5221 gtcatagcag gtttagaggg ggcaaaaaat gaaaaatgta gtaattggaa cagcaggtca
     5281 tattgaccac ggaaaaacta ctcttataaa agcattgact ggaagagaga ctgatacatt
     5341 agatgaagaa aaaaagagag gtatttcaat aaacttagga tttacttatt ttgatttacc
     5401 aaacaataga agagctggaa ttgttgatgt cccaggacac gaaaaattca taaaaaacat
     5461 gttagctgga gcatcaggaa ttgatatagt tttacttgtt gtagcagcag atgaaggggt
     5521 tatgccacag acagttgagc atttagatat tctaagcttt ttaaatatca aaaatggaat
     5581 agtggttcta actaagtctg atactgtaga tgatgaattt agagagctag taaaagaaga
     5641 tatttgtgaa aaaataaaag gaacttttct tgaagatgct gaaataattg aagttgattc
     5701 tatatctaaa agaggtatag acactcttat tgaaaaaata gataaaatga gtgatgatat
     5761 agaagataaa aatgaaaact ctccagcaag acttaatata gacagagtat tttcaataaa
     5821 agggtttggt actgtaataa caggcacttt aatagaagga aagataagta tagaagatga
     5881 tttagttata tatccacagg tattaaaaac aaaaataaga agtatacaag ttcatggaga
     5941 aaataaagat acagcttatg caggtcaaag aacagcaatc aatatatcaa atgtaaaagt
     6001 ggatgatata aaaagaggag atgttttagc tgcaccaaac tcattagagg aatcaatgat
     6061 gttagatgta aaattatcat tggtaaatca tttagataaa ggattaaagc actgggatag
     6121 attaagattg tatcatggaa ctagagagat actttgtaga gcagttccac tagataaaga
     6181 attaattgaa agtggagaaa gtggctatgt tcagttgaga cttgaagaaa gcatagtatc
     6241 taagaaaggt gatacgtttg tagtgagacg ttattctcca atggaaacta taggtggagg
     6301 tgtaataatt gacccatctc ctaaaaaaca taagaaattt gatgaaaaag ttatagaagc
     6361 attaaaaata aaagaaaaag gcgaactaaa agatataata gaagagtact taaaaaggaa
     6421 tttaaaaaat tatccaaata taaaagaaat aatgagctat agtggtgctc atgaagaaga
     6481 tgtaaaaaga gcgcttgaag cacttatatc agaagataaa gtatttatta taggaaatat
     6541 gtatatgcat ataaatcaat acaataaatt aaaagaaaat acaataaagc ttttaagtga
     6601 gtaccataag aagtataggc ttagaaaagg gatactaaaa gaagaagtaa ggtctaagat
     6661 agagagtaat tttaagacta gggagatgga tattttatta gaaaaattgt ctacagaaaa
     6721 tgctataaaa atagaaaata atatagtatc tctattagat tttgaggtta ttttaaatga
     6781 taagcaaaaa gaaatagcaa aaaaaataga aaaaagatta aaatcatgtg gagtttcttc
     6841 tatactgaca atagatgaag tttctgaagg aaatcataac tatgctgaag tattggagtc
     6901 aatgataggt aataaagtag agaaattaga tgatttgtat ataatggaca aagatattta
     6961 tgaaaatgca aagaacattt tgataaatta tataaaagaa aataaagaaa taacattagg
     7021 agaatacaga gatttgattg attctagcag aaagaattgc atgattatat tggaaaactt
     7081 cgatagaaac aaaataacta aaagagtaga aaacaaaaga attttgtttt agcagaaaat
     7141 gtctgttttt gctttaaggt ataaagaagg aattataatg tcgagtatcg tataattaat
     7201 tagtataaaa tattaattaa gaggaagtgt aaagtattga agaatataat acctgatgta
     7261 aaaccagata taaaagaaag agaagacatg agccttttat ttataataat gggctcagta
     7321 cttttattat ttattataaa agatatagag aaatatgaaa ctttattaca tgccatatat
     7381 acgatatttt tagtagctac aggagtaatt atatttaata ctacaaaatt taaagtagat
     7441 tcattttcta catttttagg attaattttt atagctactg gagtattgga gtgtatatat
     7501 ttatttaatt ccttggggat taaaactaag tctataatgg agattaacat cacaatatca
     7561 gcaataacag atttatttcc tatactgggt gtatacttaa gttttaaatt tgttaaggat
     7621 aacaaacaaa tttattcaag tataatttta tttatagcaa ctgctacatt aacaataaca
     7681 tctttattca taatatcagg aatttgtgac tatctgggaa atggtaaatc acaatatgct
     7741 ttaggagtta taatatctat attcataata attatttcta tcattagtgg aattgaatta
     7801 aattcaagtt caaataaagg taaatgggaa tatgatgaaa aaaagatttt taacagaata
     7861 atagtaataa taattttatc aagagttcca aatctattgc atatagttat tgataataga
     7921 aatgtagagc ggatattatc acagataata ataaatgtag ccttatatta tttatataat
     7981 tacatagtat ctaagaatat taagaaaaca gcactggaat tgcatgatac gaatgaagaa
     8041 ttaacaaaaa aaacggaaag tttaaaagaa aagaataaaa aattaatcta tgagacagaa
     8101 aagatagagg aacttaaggg aatacttatg aaaagagaat ctaggctaca atctacatta
     8161 gatgtagcag taaatagcat agtagtgttt agcaaagatg gagaaattac atatgcaaat
     8221 aagagcttta gaaatacttt tggagactat aaagaacaag ataaattgac tgataaaata
     8281 aaaaacttca atgatttgat agaaagcatt cattatgtgt ttgttcatga gaagaatgca
     8341 gagaaactta ttagtacaag cgataataaa gtttatcaag ctatatttgc acctttaatt
     8401 attgcttcac aaacagaagg agtattgtgt gtactagaag ataagactaa gaaaaaggag
     8461 tacgaacgca aacttattga agcaaataag agaagtaaag attttttaga aagtattgga
     8521 gatggcatag tagtgcttga aggtaataac aaaatatatg ttaataatgc atgtagggaa
     8581 atttttaaaa ataatttaga gagtattgat ttttcattag tatgtaaaag tgaagaaagt
     8641 atagaaaaaa gatatgtaat agatggtgaa gtaaagtatg tagagatgag tttttctcag
     8701 tatactaatg ctggtactaa taaaactatt attgtaatac gtgatacaac ctctcgaaaa
     8761 atatctcaaa taaaacttga ggaaagtcaa tcatcatatt caagatttat agatatatta
     8821 cctgatggca tatgcttatt aaaagaagat ttaagtataa actatgcaaa taagtcgttt
     8881 ttagatatgc tagcttttac aaacatagat gatataaaag attctaatat taaactatta
     8941 atgaatgcga atactgaaga aaaaatgaaa tttacggata atatggctaa agttttaaat
     9001 gaaaatagaa gtatactttt actggaatat gaattgataa atagttatga tgatatagta
     9061 gaagtagaat taagtgcatt accttttgct atatacaata ctagatacat aatgcttatt
     9121 ataaaagatt tagtacataa gaaatattcc gagcaagcag aaaaagaact gcttgaaaga
     9181 tttaaaacag ataaaatcaa aacggagttt tttgcaaata tgtctcatga attaaaaact
     9241 ccactaaatg ttatatctag ttcaaatcaa ctggtagatt ctttctatag aaatgaaaag
     9301 ataaaagatt acaataacaa tattaaatct catgtggatt tagttagaca aagttcttat
     9361 aggcttcaaa gattaataaa taacattata gatttaacaa agatggagtc tgggttctat
     9421 acactaaaac ttgcgaaata caatattgta tctgtaatag aagacttatt tatgaatata
     9481 gaagagtatg cacttagaaa agacataaaa attctttttg atacagacct tgaagaaatt
     9541 aatgtttata tagacaaggt tgaaattgag agaattatgt tgaatctatt gtcaaactgt
     9601 attaagttta ctgataatgg aggctggata tatgtaagta ttcattataa aatagataaa
     9661 gtaatcataa gtgtaaaaga cactggagtt ggaataccac aagataaatt agaattgatt
     9721 tttgaagaat tttcacaagt tgataagact ttatccagaa atactgaagg aagtggaata
     9781 gggcttgcta tagttaagaa tctagtaagt cttcatggtg gagatataga agtagtaagt
     9841 gaagtgaata aaggcacaga gttcttaatt tcacttccaa tgaagggatt tagtagtgaa
     9901 cattacacag aagataagag aatatataat attcaagaaa aaataaaaat agaattttct
     9961 gatatatact attaaaaatg attatgagca aaaataaaat aaaaatacta tttgtttaaa
    10021 tatttaagtt gtaagaaata ggctatcata aggtaaatta ttatgatagc ctatttttag
    10081 ctaggtttta tatctatatg tgagaacata taatctctaa attaccttca aaactaaaat
    10141 ctccaaagtc gtttggcagt ataaaatgca tacccttttt taaattatat tctacagaat
    10201 tgtgtataag ttttccattt ccatccaaca cactgtataa agaaaaaggt gtattgtgtg
    10261 taaaattaca ttttccaaaa acatctaatt tataaacact aaaaaattcg tttgacacaa
    10321 atgttgtgca tttaaagtct gatgttgata cgattttata gtcagtatcg aaatttatat
    10381 gaggaacatt cgtaacatct attgattttt gaacatgaag ttcacgctta ttacctgaat
    10441 tgtcagttct atcataatca tagactctat aagtagtatc tgagttttgt tgagtttcta
    10501 aaatcaatgt tcctttgcat atagcatgta tagtaccact tggaacatag aaaaagtctc
    10561 ccttttttat attaaccttt ctaagtagtt tgtcccattc attattattt accatattta
    10621 caaattcttt atgagatttt gcattatgac ctattataat ttctgcatca tcagaacaat
    10681 ctattacata ccaacactct gtcttaccta actctccatt ttcatttttc ttagcatatt
    10741 catcatttgg atggacttga actgataaat tatcatttgc atccaatatt tttgtaagta
    10801 aaggaaactt atcaccaggt gtgtttccaa ataattctct atttttattc cacaattcag
    10861 atagtttttt acctttatat tttccatttt ctattaagca atctccattt ttatgagagc
    10921 ttatagccca acattcacca gttgttggcg aatctatttc ataattaaat ttatctttaa
    10981 gtgcagtacc accccatatt ctatccataa aaataggttt taaaaataat ggttccataa
    11041 tcctgttcct cctatgatat taaatttatc ctacaaataa ttatatataa atagattata
    11101 aaatcaatag taaaaaagtt ttgtcccttg aaatgacata aagtataagg agttattaaa
    11161 taagataaac tatgatatat aatataaagt tctaaattgg agtgataatt atggagttgt
    11221 ttaaggcaga aagcaaaaaa tatgaaaaag aatttttaaa attattggag caatgggtta
    11281 gtataccttc attttatgat agaaaaactg tatctaaaga tatgcctttt ggaaaaggag
    11341 tatatgatgc attaaactgg tttgaaaatc taggtagaga aaacaatttc aaagtaaaaa
    11401 atatagataa tcatgctgtt caaattgaat atggaaatgg taaagaatat gtagatatat
    11461 ttggacattg tgatgttgta aatccaggtg aaggatggga tagtgaacca tttaaactaa
    11521 atattatagg tgataaatta gttgctagag gtgtatctga taataaagga cctatgatag
    11581 tgaatttttt agcactaaaa atgataaagg atttagatat taatttaaaa agaaaggtta
    11641 gattaatagc tggaggaaat gaagagagtg gatttaaatg tataaagcat tattatagca
    11701 aagagccata tggtgtatgt ggatttactc cagatgctaa atttcctgta ttaaatggag
    11761 aaaaaggtgg agcaataata aagttaattt caaatataga cgataaatct ttatatataa
    11821 gtggtggaat agagtttaat acaataccag ataaagttta tataaagaat gtagaaaaat
    11881 tgggtaaaga taatatatgt tttgatatta acaatatatc gataaattat gataatggca
    11941 attatattgt gtatggaaaa ggtggtcatt catctaaacc cgaaaaatca ataaatccta
    12001 tattagctac aattaaactt ttgtcagaaa atattgatga aaaatggaca aaagatttat
    12061 ataagttgat aaatcaagat aatataaatg gaaacttatt tggtttaaat attgagggga
    12121 aatgtgggat tttatcaata gtgccaacaa taataaatat agtagatgga aaattagaag
    12181 tagttttaag cgttagatat ccagaaatat taaccattga agatataata aaaaaattta
    12241 atttgtatat ggagcaaaat aatattaata aatttgagct tattggggaa aatttaaagc
    12301 aagcaaatta tatagataga aattcaaaac ttgtaagaag tttgcatgat atatatataa
    12361 agtattctgg agatttgaaa aatgatgtta gagtaactag tgcaggaagt tatgcttctg
    12421 aaatgaataa ttcagtaata tttggttgtg aatttcctga tggtagcttt ggcaatgtcc
    12481 atagtgcgaa tgaatttgct agcttagata gatttataac tgcaataggt atatatgcag
    12541 aggcaataat tacattatgt aataaaatat aaatacaaaa tgtcccttat atagacatat
    12601 tatatataca attaaaattt tacttgatat ataattgaaa tcaaagagaa ggtgatacaa
    12661 tgataactaa gaaacatgca ataatagtaa agagtttaag caataaagat ggatatatga
    12721 catcaaacga attagctatc aaattggatg tgtctactaa aactattaaa agatatatag
    12781 cagatttaaa tagtgtttta agcaaatatg atttggtaat agcttcttct agaggtatag
    12841 gatacaagtt aagcggttct aaaaacaata tagccagagc tgttaaagaa gcaaataaat
    12901 atattgatgg ttttttagat gactctgaag agtcaagaat gtcaaatata atatgtatgc
    12961 ttattaatag aaattatatg agtatagaag caatggcaga agaattaaat ctaagtatag
    13021 ccgctataaa taaattgtct agtaagctta aaagaaaact tgaaaagtat gacttagtta
    13081 taaaatctaa accttactat ggaagctata tacatggtga agaaattaat ataaggcaat
    13141 taattactga ctatgcaata aaattagatg aaaaaagtaa agtgaaggtg tttttagatg
    13201 acattagtga aaacgatata tactgtatag aaagtatttt agaaaagcac ttaagagaaa
    13261 aagatacaat aatttcagat aaagatttta atcttttgct ttcaaaaata attgtttctg
    13321 tatttaggag taagagaggt catagtaaca atataaactt aatggataca agttatagat
    13381 ttcataacta ctactttata gaaaatctaa tgaaagaaat atctgataaa attggattta
    13441 aacttattga agatgaagta atttatattt caaattattc tggtgtaata gcttataaag
    13501 gtacacaagg aagaaaaaac acaagtgaaa ttgaggaacg aataagtagt gttataagta
    13561 gtgcacttca agatatattc cttatttcag gtagtgatta tacaaaagat gatgagttta
    13621 tggttgcaat ctctgaccat ataaagagat ttttaaatag agctagagct aatgtaaaat
    13681 caaataatcc tttacttcat caaataaaag agaagtttcc tatagctttt aatttagccg
    13741 tgtttttatc aaataaacta gaaactgagt ttaatttaaa acttgatgaa gatgagcttg
    13801 gatatatagc tatccacttt gctgcttcaa atgaaagaat gaaaaaaaat actagtaaga
    13861 aaatttgtat agtttgtcat tatgggatag gaacaggaca acttatttct gaaaagctaa
    13921 aacagaatat aagtgattta agtgttgtag gtgtttatcc agttagatat cttgatatgg
    13981 ctataagtca agatgttgat ttgatagtat ctaccgttga attaaaagga tatgaaaaac
    14041 ctgtcttata tatagaaaat atatttgatg atagcttaat tgaaaacgta aacaaagcct
    14101 tttatgaaaa ggaagaaaga agaaagataa taagtaacat gtttgatgaa aaagcttttt
    14161 tcagtataaa ggctagtact aaagaagagg tcataatttc actttcaaat aaactaaaag
    14221 aaagagattt tatagaggaa agttctataa aaagtataat agatagagaa aatatttcat
    14281 caactgaaat aggaaatcta gtagcaatac ctcacacaat agtgaaggga gataaaaaat
    14341 ctataatagg agttggaata cttgaaaatc caataatttg ggataagcaa gaggttcagc
    14401 tagtatttat ggtatttttt aatactaaag aaaagcataa tttctcgata tttaaatacc
    14461 tttataattt tataaaagat gaaggaggag taagaggaat tattaaaata tgtgacttta
    14521 ataaattaat ggcactgata ggtaattaag gaggaaatta ataatgggaa caaacatatt
    14581 taacaaagaa tatgtatttt taaatgttga tgctaagtca aaggttgaag ttttaaaatt
    14641 tatatcaaaa aaagctaaag acttaaattt ggcagaagat gagagcttag tatatgaagg
    14701 tttaatggct agagaagaac aatttactac aaatttagga gaatctatag caatacctca
    14761 cactaaaaat gatgcaattg aaaatccagc agtagtagta ttgaagttta atgaagatgt
    14821 tgtttggaat gaaggtgagg ataaggttaa attagcaata agcttactta tgccaggaaa
    14881 aagtaaagag aatattcatc ttaaattatt atcatctctt tcaagaaaat taataaataa
    14941 agaatttaaa gattctcttt taaaaagtga taatgttgaa gaaatatcta acttaataaa
    15001 tgaagcttta ggtttataat taaatactaa ggttataaaa taaaaaaata attaactaaa
    15061 aataggagga atttgaaatg aataaaaaat tagtagcatt atgtgcttgc ccaatgggac
    15121 tagctcacac tttcatggca gcagaagcaa tagagcaagc agcaaaggca ttaggttatg
    15181 aagcaaaagt tgaaactcaa ggtgctgatg gtgtacaaaa tgagttaaca agagatgata
    15241 tattaggagc aactatgata atacatgcag tagcaataac accagaaggt atggaaagat
    15301 ttgatggttg tgaagtttat gaagttgaat tacaagaagc tataaaaaat gcagaaggtg
    15361 ttataaaaga aatagaagaa gatttaggaa tataataaat ttattgtgga ggaaatagaa
    15421 tggcaattaa gaaaaaagtt ataaacagtt caggtgcttc tacaacagga ggaaataatg
    15481 taggaaaatc tactccatct agtccaactc aatcaaaggg aaatggaaaa tggaaagaaa
    15541 taagtaaaca tataatgaca ggtatatcat atatgatacc agttttagta atgggtggac
    15601 ttataggggc cttatctcaa ttaataccat atgctatatt aggacttgac ccatctgtag
    15661 gtatagttga tgctatgaac tcaggagaat ttacagggtt caagcttagt cttttaaata
    15721 tagcacaatt aatgtcaaat ttcggattta ctttgtttgg atttgcgata ccactgtttg
    15781 cagcattttg tgcaaactct ataggtggaa aaacagcttt aattgcagga tttataggtg
    15841 gatatatagc taataaacct gtaggtgtac cacaattcat tgatggacaa tggactgaag
    15901 ttgttccagt tgcatcagga tttttaggtg caatactaat agcattcata ataggatatt
    15961 ttgtaaaatg gttaaataaa tctataaaag tttctcataa ctggttagct tttaaaacaa
    16021 catttttaat accattaata gcgtcattag cttgtatggt attgatgata tttataataa
    16081 ctccatttgg tggattaatt aatgaaagta tgaaaaactt cttaacagca gcaggagcag
    16141 ctggagaata tgtatatgca acagctttgg cagcagctac agcattcgac ttaggaggac
    16201 caataaataa agcagcagga ttcgttgcac ttggtttaac tacagagaat gtacttccaa
    16261 taacagcaag aacaatagct atagttgttc ctccaattgg attaggatta acaacattat
    16321 tagataaaag attagtagga agaagagtat atgatagaca attctatcaa gcaggaaaaa
    16381 cttctatatt cttatcattt atgggtatat ctgaaggagc aataccattt gcacttgaga
    16441 gaccaggttt tgttatacct ttaaacatag ttggaagtgt aataggagct attacaggta
    16501 taattctagg agcaattcaa tggttcccag agtcagctat atgggcatgg cctctagtag
    16561 ataacttatt tggatatata ataggtatag cagttggtgc tatattcata gcagtaggaa
    16621 atatattcta tagaaataaa ttaataaaag atggtaaact tgttgtagat tatatcgact
    16681 agtatttaat ttttactatc tataaataac tttggctggt aatatggttt taatcaatat
    16741 taccagcctt acctatatga ttaattaaag gcggtggaga taagatatgg ataagataga
    16801 aaaagtaaga gagtatttta gagaatatga tatagatgga tttcttataa atagtcctac
    16861 aaacaagttt tatgttggaa atttatttag ctcttcaggt tatgttttta taacgaaaga
    16921 atcacaatat ataatagtag attttagata ttttgaagaa attaaaagaa aatctagttt
    16981 gtttaatgta gtattaatgg acaaaactag aactcatttt gatataatta atgatatttg
    17041 tagagagcag aatataaagg aaattggatt tgagggaaat gaagtatctt ttgatttata
    17101 tagaagtatg agtaataagt taagtgcaac cttaaaatca gttgatttat ctacattaag
    17161 ggaaacaaaa aatgaagacg aaataaaata tataaaaaaa gcttgtgaaa tagttgatgc
    17221 tacattttat catatagtag actttataaa gattggtatg actgaaaagc aagttgaaaa
    17281 tgaaattgtt agaattataa aagatttagg tggtcaaaaa gaatctttcg atacaatagt
    17341 tgcatctggg cttagaggag cattacctca tggaaaagca agtgaaaaaa ttatagagta
    17401 tggagatttt gttacttttg actttggtgc taagtataat aattattgtt cagatattac
    17461 cagaaccatt tgtatgggta ctataaataa agagttagaa gaaatatata atatagtaag
    17521 aaaagctaat gaagagtgta taagagtttt aagaccggga atgactactg gagagataga
    17581 taaagttgca agagacataa ttggttcata tggatatgcc aataattttg gtcataatct
    17641 gggtcatggt gtaggaatta tggttcatga atatcctgcc ttagctcctg aatcaaatga
    17701 agtattaaaa gaaggaatga ttgtaactat agagcctggt atatatgttc caagtcttgg
    17761 aggcgtaaga atagaagatg atgtactcat aacacaagat ggatgtatga gattgactac
    17821 atctactaag gatttaatag ttgtaagcta aaaataaatt agataaatat tttataacaa
    17881 taagaaaaga agaggtaaat ttaaatgtat aattttgatg ataaaccaga tagagtaagt
    17941 gaaaagtgta gaaaatggga tttaaatatt ataagagata aatttggaga tattagagaa
    18001 gattttgtac caatgtggat tgctgatatg gattttaaaa taccaacaga aatagaaaac
    18061 aaatttattg aagctgtaag aagaggtgta tttggatata catactgtta tgatgaattt
    18121 tatgattcag ttattggttg gcaaaaaaat atgcatgagg taaatgtaga aaaagaatgg
    18181 attacattaa cttatggaac tgtatcaaca cttcattatg tagtacaagc tttttgtaag
    18241 aaaggtgata gtataatatt aaacacacca gtttatgacc cctttgaatc atcagcaaaa
    18301 aaacaaggtg taaatgtaat ttgtaataca ttagatgttg taaatgatag atattatata
    18361 aattttgata aattagaagt tcaaattaaa gaaaataagc ctaaattaat gatgttttgt
    18421 acaccacata atccatctgg aaggatatgg acaattgaag aaatgacaag ggtagcaact
    18481 atatgtaaag aaaataatgt tattttggtt gcagatgaag tacatgcaga gcatattcat
    18541 tatggtaaat ttaattcaat acttaagatt ggaaaagagc ttttagaaaa tgttattcta
    18601 ctgacatcac caaataaggg ctttaatctt ggtggattaa agacttctta ttcaatagtt
    18661 ataaataaga atataaggga taaatttaga aataaattaa agcaaaattc tataacttct
    18721 ccaaatgtgt ttggaataat aggattgata accgcttata atgaatgtca tgagtggtta
    18781 agtggtgtaa atgaatatat aaaatcaaat tatgaacttt tagaaacttg ggtaaataaa
    18841 tataataaga taaaattgat gaaaatggaa tcatcttact tagcttggat ggatataagt
    18901 ggattaggta taagtgcaag tgagtttaca gataaattgg ctatagatac aggagtatta
    18961 ttagaagatg ggagtcattt tgtaaaagat ggtgagaagt atgttagaat aaatttagga
    19021 actcaaaagg aaaatgtaat tgaagcttta aatagaatgg atttattttt aaagtcttta
    19081 taaaaatttg gttgaattag tattttatat aaatttaagc tattaatatg gtatatacat
    19141 aagtagaatt tgttttagat tacttaagta tataccatat tttatataaa aattaaattt
    19201 gcattagtca tgtgataact atttaaattc atatgtattt tgtatgtata atagatataa
    19261 ttattattta aacttattgt ttatttaatt tttcaataga aaaagattta tctgtaagtt
    19321 taaaattatc ttttataccc ttagttaaat atactttctt atccttagtt atgaatatag
    19381 catctacatt atcaatttca ttaattaaat ccataccttt ttcaagacca agtccaaatg
    19441 tagaagtgga taaggcatca caattaatag atttatcaga aattattgta acactactta
    19501 gattgttttc aaaaggatac cctgtaaatg gatttaacat atgatgatat atttttccgt
    19561 ctttttcgat aaatctttca taaattccag aagtaacaac agatttattt gtggtttcta
    19621 tgtttcctat ggattcacca tttttactag tggggtcttg aatgcctatt ttaaaaggtt
    19681 tatcattttt accatcttct aaagtaaata tatttcctcc taagtttact aaaccttttt
    19741 ttacgtcctc tgattttaaa taggctacaa ttttatcagc agcatatcct ttagctatgg
    19801 cacctaaatc aattttcata ttttctttgg ttaattttat agatttattt ttttcatcca
    19861 atatgatgtt cttataatca ataagaggca gaagtttttg tatctcagaa tcacttggaa
    19921 ctttagcttt atctgttcca atagcccata aatttgataa tggaccaatt gttatgtcaa
    19981 aatatccatc tgataatttg gaatattgta tagcttcttt tacgacgaaa aatgtatcat
    20041 cagaaactct gacaaatttt ttaccagcat tatcatttat tttagaaaca tcacttcctg
    20101 gtatatgagt gctcattttg ttgtcaatat atcttaaaat tgaatcacat tcatttaaga
    20161 ttttatcact tttggattct tttacattaa atatagtaac ttcgtttact gtaccaaggt
    20221 agtagctagt tttactatat ttagtattct ggcttgaact cctaaaaaat gaaaataaaa
    20281 ttaatgctaa aatcaatatc acaaaaataa atgtgatttt tttcttattc atggtatatt
    20341 cctcctatga aattgtgtta ttattttatt ataacataat ttcataggag gacaaaaaat
    20401 gaagaaaaaa gacattcttc ttattttagg cattttggtt gttattatag catgttatgg
    20461 gataataaat gttataaatt ctaaaaatgc tggaaatatt gagatttatg tagataataa
    20521 attgtataaa actgtttcaa taaatgccaa agaagaattt aaaatagaga atagaggggg
    20581 gtataacata gtaaagattc atgataaagg ggttgagata gtagatgctt catgccctga
    20641 taaggtttgc gtacatactg gatttataaa caagccaagt caaagcatag tttgtatacc
    20701 taatagagtg agcataaaaa taaatacaaa tgaaaagaat gataatcaag aagatataat
    20761 ttctaaataa aataataaaa ataattgaaa ggaattggtg ataattatgg ttaacttagg
    20821 aaacgattgg gatgaattat tgaaagaaga atttgaaaaa gattactact taaacttaag
    20881 aaaatttctt atagaagaat ataaaacacg ccaaattttc ccaaatatgc acaatatata
    20941 tgaagcttta aaacacactt catacaaaga cacaaaagtc ttaatattag gacaagaccc
    21001 ttatcatgga gataatcaag ctcatggttt agctttttca gttcaaccac aagtgaaaac
    21061 tccaccatct ctattaaata tgtataaaga attaaaagat gatttaggtt gttttatacc
    21121 taataatgga tatttaatgc cttgggcaga ccaaggagtg ttacttttaa atactgctct
    21181 tactgttaga gcgcatgaag ctaattctca taagaacaaa ggatgggaaa ttttcactga
    21241 tagagtaata tctattttaa gtgagcgtga agacccagtt atttttgtac tatggggaag
    21301 taatgcacgt aaaaaagtag agttgattga tacaagtaaa cattatattt tggaagcacc
    21361 tcatccaagc ccattgtcag caagtaaagg cttttttgga tgtaaacact tctctaagat
    21421 aaatgaaatt ttgaaaaagc taggaaaaga acctattaat tggcagatag aaaatatata
    21481 aaagtaaaag gtaacaaaat gataacaaaa caacaataaa caatgacatt gttgtaacta
    21541 atagaaatat gcttaaatag ttgaaaatac tatgtatgca tatttctatt tttttgtcaa
    21601 gttgacaact cttgaaaaaa tagttacaat ttagttacac aaaaaacaca aataaaaaaa
    21661 gaaacttaca aagcagaatt ttatgaagca aaatatatag tgtaaaaaat tttagggagg
    21721 aaattttata tgaattttaa aaaattgagt aatgctaaag tctcggtaat ggcagttgtt
    21781 ttatcagttg gaatattatc gggatactct gcgcttaata aacaagtaac attagtagta
    21841 aggggagaag aaagaactat atcaacattt agttctaatg taggagacct tttagaatct
    21901 caaaacatta gctatgatgc aaatgatata gtaactttag atgaaaatac aaagttatct
    21961 aatggagata aaatagaagt aattgatgta aaggaaaaaa cagttgtaga aactaaagac
    22021 attccatttg aagttacagt tgttgaagat ggctctttat taaagggtga ttctaaggtt
    22081 aaagaagaag gaaagtctgg taaaagtgaa ttaatatata aagaaactta tcacaatgga
    22141 aagaaagttg aaaaggaatt tgtgaaaaag gttgttaaag ctgagccagt aaataaggtt
    22201 gtaagcaaag ggacaaaggt agaaatgcaa gtagcctcat caaggggaga aagttcaaga
    22261 agaatggctt catcatcatc tagtagcagt agtagtaaca atggtaaaaa tatgagagtg
    22321 gtagcaactg catatactgg tcatagtata actgcaacag gaacaaaacc taagtgggga
    22381 acaatagctg tagaccctaa ggttatacca tatggaacta aagtttatat acctcaattt
    22441 gacatggtgt ttactgcaga agactgtggt ggagcaataa aaggaaataa aattgatatc
    22501 tacatgaatg attcaaaaac ggtttataac tggggaagaa aaactataga tatacgtata
    22561 ttaaaatagt taaataattt atatttttac tggaaattat attttgatta tatagagaag
    22621 aaactcttta tagattctta aaatataatt tccagttttt attttggata tgtaataaaa
    22681 caggtaaatt ttaaccatgt atagataaaa gctatttaaa ttataattta ctatcagata
    22741 aatctataaa aattgttgca aagagtcaaa atatatgata ttatagattt cgttgagaag
    22801 gatttcatta tgggagtgga tgggtgccgg tgtgccctct agtcttcaaa actagtatga
    22861 ggggttaaga gcctcttagg tgggttcgat tcccacgcat tcccgccaat atgcaaaagg
    22921 tatcttttaa ttaaaagata cctttttaaa tttattttaa gataatttat ttttaataat
    22981 atataatgtt ttgtattata taatgtaaat tttctaatac tattatatat ttagataaaa
    23041 atctaggagt gtggaaagat ggaaaataat tttacaacga gaactagttt tctagttgga
    23101 gatgatggga tagaaaaact taataattca aatataatag tatttggagt tggaggagtt
    23161 ggtagtttta ctgttgaagc tttagcaaga gctggagtag gcaatattac tattgtagat
    23221 tttgatgatg tagatataac caatataaat agacaaatac cagccctaca ttcaactgta
    23281 ggaaggtaca aagttgatgt catggaagaa agaatattag atataaatcc taatatcaat
    23341 ataaaaaaaa taagaagtct ttataataaa gatactagtg acgaaatact aactgaaaga
    23401 tatgattatg ttgtggatgc gatagacatg gtaagttcca aaatacatct aattgaaact
    23461 tgtgaaaaaa aagggttgaa aataataagt tctatgggta tgggaaataa attagaccca
    23521 actaaaatag tcgttacaga tatacacaaa acaagtactt gtcctctagc taaagttatg
    23581 aggaaagaac ttagggatag aggtatcaaa aagttaaaag tagtatattc tactgaacaa
    23641 ccaatagaac ttaagaagaa agtaatgaat ggtagaaaag taacacctgg tagtgtatca
    23701 tttgtcccat ctgtaggagg tttaataatc gcatctgtaa tagtaaatga actattagga
    23761 caataagttt taaatatttt aatttataaa ttttatatac aaatttgaag gctagaagca
    23821 tgaatataaa gcaattttat attcatactt ttagcctaat tattttatat taccttattt
    23881 tatgtagttg tagttttatt ttttagatta tgtatctatt tcataattat attttgtaaa
    23941 tataatttct ccatcactat aaaaaacaat atctcctatt ctatctgttc tataaattga
    24001 tacatggttt tgttttagat tattaataac ctccctatgt ggatgaccat attgattttt
    24061 atatccacat gatattgcag ctatatctgg actaattttt tttataaatt ctaaagatga
    24121 agaagaatta cttccatgat gacctatttt tataaaatta atattattta atttaaaaga
    24181 atgtaaaatt tctttttcat tttcttcttc agcatcaccc ataaataaaa aagaattatc
    24241 gttaaaagtt aatttgaaaa ctatagaatt taaattagat tcttcttgta tatatgatgg
    24301 acttaataca tatatattta tattgttatc tatgtttaaa acatcatttt tatagagatg
    24361 ctgaatagat aagtttttat ccgtacaaga atttattaga ttttgataag cctcactatc
    24421 agttgattgt tcaggcatat atatggagtt tacattaaat ttttttataa cattatcaag
    24481 acttcctatg tggtctgaat ctggatgagt tgctattata atatcaatag tttttattct
    24541 tttttgtcta agataactaa taattatatt ttcgctatct tcatcacctc catcaattaa
    24601 tatattctta tttgtgggtg tttgaactaa aatactatca ccttgtccaa catcaattat
    24661 atgtatagat agtagagatt ttttgtcaca gccgattaaa agtgatatta taataagtat
    24721 tgataaaaaa attttatttc tcaataaaaa atcactccat tcattacatg tttatataaa
    24781 tttatgatgg gtaatataag atatatatga cttattatat tttatatttt aaacaatata
    24841 aatgaaatta ataccataat aaatttggag ttttttatat atacaaaata aaatttttaa
    24901 aattaagcaa taaaaaaggt aggaggattt tatgtttttc aaagatattg aactaaattc
    24961 aaaaaaagag ttagacccat attttgattt agtagattat gaagcttgtg agtactgttt
    25021 tagtacttta tatatgtggc aacatgttta taagacagga tattatatag gtgaagattt
    25081 tgccgtttta gttggggaat acgaaggaga tagtttctca atattaccac ttgctaaaaa
    25141 agataaactt ccagaagttg tagattttgt ccttgaatat ttttctaaaa ataataaaaa
    25201 aatatactta agaggtatta ctactgaagt agtagagttt ttaaaagaaa aatatccagg
    25261 tagatttgag tatatagaag aaagagattt atttgactat atttatgatg cagaaagttt
    25321 aagaactcta gctggtaaaa agaatcaaaa gaagagaaac catataaact atttcttaaa
    25381 agagtatgca ggtagatatg aagctaaatt attagataaa gaaaactttg acgagtgttt
    25441 agtactaatg aaggaatggg aaagtaataa ggaagaaaat aatgaatttg atgaaagtat
    25501 ggatgacgaa ttaataggta tcaaaaagat atttaaccat tatgacatat taaaagataa
    25561 ggtaaaagtg tttggtgttt atgtggatgg aaaattagag gcttttagta taggagaact
    25621 tttaaatcca aatatggcat tgattcatat agaaaaagct aatcccgata taagaggtct
    25681 gtatccattt ataaatcaac aatttttggt aagtgaattt aaggacgttg agtttgtaaa
    25741 tagagaagaa gatttaggta ttgaaggatt aagaaaagct aaactttctt atcatccttg
    25801 cagatttgtt gaaaagtaca gtgttaggga ggcttaatag actatatgga gattaggtat
    25861 gctaaagaag aagagataga aagtataaaa gagatttgga gttattgttt taacgatacg
    25921 gagagtttta tgaagtacta ttttaatgat aaatataaat ctgaaaatac tgtagtagct
    25981 ttagatgaag gaaaaattat ttcttctctt cagctaaatc agtataagtt attactaaat
    26041 agcaaggtat ataatacatc atatgtagta ggagtttcta cacttccaga gggaagagga
    26101 gctggatata tgaacaaagt aatgaaattt actttaaacg aattgtataa aaaagggcaa
    26161 ttagtttcta tattgatgcc tattgactat agactttata gaagatttgg atatgagcat
    26221 tgttacgacc agattgaata tactattaac acagatgatt taaaaaattt taagtcaagt
    26281 ggaaaaatga taaagtctaa tttatcacaa attgatgatt tgatacgaat agatagaact
    26341 tttttaaatg aggttaatgg taatgtgtta aaagatgaac attattatga aaacttattt
    26401 aaagagattc aaagtgaaga tggattttta tacattcatg aaggaaatga aaaagatgga
    26461 tatatagttt attttcttca ggaagataaa atgtttgtca gagagttatt ttataaaaat
    26521 atagatgccc ttaaatcaat gcttaaattt atatataatc acaatactca atgtaagata
    26581 gttactatat ctactcctac aatagataag ataagattta tattagacaa ccctaaagat
    26641 agtgatataa aaataaaacc ttttatgatg ggaagagtta taaatgtaaa gaagtttatt
    26701 gaagatatag atattgagaa ggatattaat agctcattta atttattaat agaagacaag
    26761 tttatagatg aaaataatgg tctatttaaa atttctatac agaataagaa ggtatctgta
    26821 gagcagttag ataaaaaagg tgcagaaaag cctcaagaag attttgatat taaattagat
    26881 ataaatacct taacacagtt atcattttct tatatagatg taaatgaagc tattttctta
    26941 aatgatataa aagatgtttc agaagaaaca ctagaaacac ttaattgtat tttttctaaa
    27001 aaaaatacct a
//
LOCUS       DAELXX010000051        26756 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.051, whole genome shotgun sequence.
ACCESSION   DAELXX010000051 DAELXX010000000
VERSION     DAELXX010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26756
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.051"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(304..717)
                     /locus_tag="KPH29_001995"
     CDS             complement(304..717)
                     /locus_tag="KPH29_001995"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBG1467589.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
     gene            complement(758..1888)
                     /gene="rodA"
                     /locus_tag="KPH29_001996"
     CDS             complement(758..1888)
                     /gene="rodA"
                     /locus_tag="KPH29_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HBG1467590.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            complement(1973..2257)
                     /gene="minE"
                     /locus_tag="KPH29_001997"
     CDS             complement(1973..2257)
                     /gene="minE"
                     /locus_tag="KPH29_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBG1467591.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            complement(2275..3072)
                     /gene="minD"
                     /locus_tag="KPH29_001998"
     CDS             complement(2275..3072)
                     /gene="minD"
                     /locus_tag="KPH29_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBG1467592.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            complement(3096..3779)
                     /locus_tag="KPH29_001999"
     CDS             complement(3096..3779)
                     /locus_tag="KPH29_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBG1467593.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYENESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            complement(3877..6855)
                     /locus_tag="KPH29_002000"
     CDS             complement(3877..6855)
                     /locus_tag="KPH29_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBG1467594.1"
                     /translation="MEENKQVDRLKIIKIVMSVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMLTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKMNVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            complement(6867..7355)
                     /gene="mreD"
                     /locus_tag="KPH29_002001"
     CDS             complement(6867..7355)
                     /gene="mreD"
                     /locus_tag="KPH29_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBG1467595.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            complement(7370..8263)
                     /gene="mreC"
                     /locus_tag="KPH29_002002"
     CDS             complement(7370..8263)
                     /gene="mreC"
                     /locus_tag="KPH29_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBG1467596.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKDMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            complement(8273..9283)
                     /locus_tag="KPH29_002003"
     CDS             complement(8273..9283)
                     /locus_tag="KPH29_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBG1467597.1"
                     /translation="MTKDMGIDLGTANTLVYIKGQGIVVREPSVVAIRDDSKEVLAVG
                     EEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQKAADKKGVVSPRIAICVP
                     FGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVEEPEGNMVVDIGGGTSEI
                     AVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERTAENVKINIGSTFKDDEE
                     INMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIKSTLERTPPELASDIMEN
                     GIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTGKSVEDQEIFEKVLMMNT
                     KN"
     gene            complement(9404..10063)
                     /gene="radC"
                     /locus_tag="KPH29_002004"
     CDS             complement(9404..10063)
                     /gene="radC"
                     /locus_tag="KPH29_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBG1467598.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            complement(10108..10692)
                     /gene="maf"
                     /locus_tag="KPH29_002005"
     CDS             complement(10108..10692)
                     /gene="maf"
                     /locus_tag="KPH29_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBG1467599.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            complement(10912..11883)
                     /locus_tag="KPH29_002006"
     CDS             complement(10912..11883)
                     /locus_tag="KPH29_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HBG1467600.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            complement(11899..12474)
                     /gene="rsxA"
                     /locus_tag="KPH29_002007"
     CDS             complement(11899..12474)
                     /gene="rsxA"
                     /locus_tag="KPH29_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBG1467601.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            complement(12489..13082)
                     /locus_tag="KPH29_002008"
     CDS             complement(12489..13082)
                     /locus_tag="KPH29_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HBG1467602.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            complement(13095..13664)
                     /locus_tag="KPH29_002009"
     CDS             complement(13095..13664)
                     /locus_tag="KPH29_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HBG1467603.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQENNEL
                     RKAVLPEAKEFKEMDKSTYKDLGDGLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            complement(13664..14641)
                     /locus_tag="KPH29_002010"
     CDS             complement(13664..14641)
                     /locus_tag="KPH29_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HBG1467604.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADLSSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            complement(14664..16001)
                     /gene="rsxC"
                     /locus_tag="KPH29_002011"
     CDS             complement(14664..16001)
                     /gene="rsxC"
                     /locus_tag="KPH29_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBG1467605.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            complement(16217..17161)
                     /locus_tag="KPH29_002012"
     CDS             complement(16217..17161)
                     /locus_tag="KPH29_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467606.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            complement(17272..18546)
                     /locus_tag="KPH29_002013"
     CDS             complement(17272..18546)
                     /locus_tag="KPH29_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG1467607.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSNQNNPTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDVVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKSKNNGWYKVKLSSGTIGWVSASYISETNEGTKEKPNSSSN
                     QNSQSNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            complement(18678..19049)
                     /locus_tag="KPH29_002014"
     CDS             complement(18678..19049)
                     /locus_tag="KPH29_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG1467608.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            complement(19277..19876)
                     /locus_tag="KPH29_002015"
     CDS             complement(19277..19876)
                     /locus_tag="KPH29_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467609.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            complement(20077..20289)
                     /locus_tag="KPH29_002016"
     CDS             complement(20077..20289)
                     /locus_tag="KPH29_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HBG1467610.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDISIEKLKEVIAELGFELKGIE"
     gene            complement(20362..22077)
                     /locus_tag="KPH29_002017"
     CDS             complement(20362..22077)
                     /locus_tag="KPH29_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG1467611.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNVTSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEEARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANINSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKAKKAEE
                     DRIKKEAEKKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            complement(22077..22631)
                     /locus_tag="KPH29_002018"
     CDS             complement(22077..22631)
                     /locus_tag="KPH29_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBG1467612.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            complement(22624..23226)
                     /locus_tag="KPH29_002019"
     CDS             complement(22624..23226)
                     /locus_tag="KPH29_002019"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBG1467613.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNISIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNSLTHPRIREKIISEIEELR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSKQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            complement(23235..25883)
                     /gene="polA"
                     /locus_tag="KPH29_002020"
     CDS             complement(23235..25883)
                     /gene="polA"
                     /locus_tag="KPH29_002020"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBG1467614.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKEYKAGRKGMPDELAEQLQPLKDLLDKFKINR
                     LEIDGYEADDIIGTVSKKAENNGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPAQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMVFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDTEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETIPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            26202..26284
                     /locus_tag="KPH29_002021"
     tRNA            26202..26284
                     /locus_tag="KPH29_002021"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26236..26238,aa:Leu,seq:caa)
BASE COUNT         8053 a         5168 c         2953 g        10582 t
ORIGIN      
        1 aataaatttt tgttttatta atgttgtcgt aacaacattt acttggttga aatagtatta
       61 cctataagtt taaatatata taataataga cttaattcta tttcatatta ttaaaataga
      121 gtaatagttt aaattctact actctatcta taatctattt cctaattatt taatctcaaa
      181 tttagctcct catcatttaa atctttcata agatttctac aggtactagg tttattgcaa
      241 cagaaaaact agggcattaa acacttatat atctgtttct ttagactgta atattaatat
      301 tttttaaata cgttgacttt tgctttttct aacttctctc catgatagtt ctcctctatc
      361 aagagctttt ataaatattt ctgctgaagc tagatttgta gctattggaa catgataaac
      421 atcacaaagt cttattaacg cttgtatatc tgtttcatga gcctgagatg tcaatgggtc
      481 tcttaaaaaa ataactaaat ctatttcttg tgttactatt aaagaaccta tttgttggtc
      541 tccacctaaa ggaccagatg ctaatctatt tatatttaat tcagtttcat ccattattct
      601 ttttcctgta gttccagttg catatagtcc atactttttt aaaattgatt cataacctat
      661 acaaaatccg accatagtgt ttttcatttg gtcatgtgct actaatgcta tattcatata
      721 tctaatctcc tcaatttatg tttagatgta tttaaattta gaaatttatt ttctttcttc
      781 tcatacacac atttagtact aatcctaagt ttgctaaaga tgtcaataac gaacttcctc
      841 cataacttac aaaaggtaat gttactccag ttactggtat taacgcaacc gtcataccta
      901 tattttggat tatctggtaa ccaaacattc ccataacacc aaccactatg agggttccat
      961 aaaaatcttt tgcatctctt gcaattgcta aaagccttaa taggaatatc ataaataaga
     1021 ttatcaacac tgccattcct attacaccaa gttcttcacc tacaacagca aatataaaat
     1081 cactatcttg tacaggtaaa aaatcttctt gattttgact tccattgtaa agtcctttac
     1141 cagttacgcc cccagaacca attgcaatca gagattgcat aacttgatag tttcctttta
     1201 gagttacatc ttctggattt agaaaagctt ctattcttac cttttgatgg tctgccatca
     1261 ttaaatacat cagtggcatt gacactaata gtatgattat tcctcttttt atcaactttg
     1321 agctaagacc tgctgtgaac agcattgcaa atatcataca acaaaataca attcctgttc
     1381 ccaagtctgg ttgtgcaatc aataatccga taaatggaag agaataaact acaacaggca
     1441 taacttcctt taacgtattt aatttatcct ttttggattc aagtatcttt gcataactta
     1501 gtacaaatgt taatttgact atttcagatg tctgcaagtc aagtggtcct aagttaatcc
     1561 acgacctagc tccacctttt actgtcccaa ttccaggtaa taaaactatg gctaacaaaa
     1621 taagacttat tatataaagc gctttataat acctgcctag aagattatag tcaaaaaata
     1681 atatgactat aatcattcct ataccaagta caaaagctaa tccttgttta atcaattgat
     1741 tatatgaacc tgttgaatta gcatgtgttg cactactaag tataacaagc ccaaaaataa
     1801 agatagctaa tactgtaact ataagtttcc agtctaactg ttttatcaac ttaattgtcg
     1861 acttataatt aatattgatt tttttcaaag aatcaccgtc tttctatctt taaacatata
     1921 attaaataaa aggggcatat accaataagg atatccccct tttaagcact aattacatac
     1981 atctatcttt tatattcttt agtggtatat tagcaactaa tgcagataca ggtttgtcat
     2041 catgctcacc tctgcattta gacattttta tttcaagacc atcatcttct atttctgcat
     2101 aattagctat tacttttaaa atgtctgttt ttatcatctc taaaagttgt ggtgaacaat
     2161 ctactctatc atgaactaaa actagcttta acctctcttt agcaacagat ttactagttt
     2221 tagcctcgtt agaaaaaact ctaaataaat ctaacacaga tgtatcctcc tttgctactt
     2281 agccatgcta aacatttttt taagtctacc aaagaatcca tcttcaactt caagatcaag
     2341 tagaggaact tcttcattta gtatcctttt tgcgatattt ttgtatgctt gaccagcaag
     2401 tgacttagaa tcaagtatag ctggctctcc tttatttgtt gatataatta tgctttcatc
     2461 atcaggaaca agacctaaca aatctattgc taatatttct attatatctt gtttatccat
     2521 catgtctcca cgcttaacca tatcatttct aattctattt ataactaatc ttatctcttt
     2581 tatttcattt gcttctaata gacctattat tctatcagca tctcttactg ctgatatttc
     2641 tggattagta actacaatag ctctatctgc cccagctact gcatttttaa agccttgctc
     2701 aatacctgct ggacagtcta ttattatata ctcgaaagat tcttttagtt tctcacacag
     2761 atcaatcatc tgctcaactg agacagcatt tttatctcta gtttgtgctg ctggtaataa
     2821 atatagatta tcaaatcttt tgtcttttat tagagcttgc ttaagcctac aagttccttc
     2881 aactacatct actatgtcat aaactattcg attttcaaga cccattacta catctaagtt
     2941 tctaagtcca atatctgcat ccacaactac tgttttttta ttttctaggc ttagcgcagt
     3001 tcctaagttt gctgcggtag tagtttttcc aaccccacct ttaccagatg ttataactat
     3061 aacttcgctc ataatactac cccctatttt tctttctatt tgtctaactt tgacaaatag
     3121 ctttcgatta caattcttcc attacttaca aaggctattt ctgggctaat ttctgattca
     3181 ttttcataat tttcttcatc atctggagct tctgcaatat tatctgctat ttgtaatatt
     3241 ttaggattaa gatttccagc aacaacatat gcaaattcat tgccttctgc acctgcatgt
     3301 acaaaacctt ctattttacc cattacaaaa acattacctc ctgctacaac cttagcacca
     3361 gcatttatat ctcccataac aactgtattt gattttgaaa ggacttttga accaggtttc
     3421 atatcattca atataactac atcaccttca aattcaatat tttcaccaga tcttagggta
     3481 ttgacatatt ttgttggaaa attactattt tcttcaattt tttgatcttc aacaaattcc
     3541 acatcaaatc tagaagtaat tccttcttta agttctagaa tctccatgtc agttaaacaa
     3601 tcactattaa ttttactaat tttagcccca ttaaaaaagc caacatatgc ttccaatttg
     3661 tttattattt tttcctgtat ttcctcaaat ggagcttctc tttttatatt tactattatc
     3721 cctcttttat tacccttgaa ctctactagt tcttgactac aaatttctct taaagacata
     3781 acaacctcca gagtgatata tcatttatat tcttcaaata cgctttaaac ccttttattt
     3841 tttctatata aagctaaagt ttatacatag attaagctat ctattaattt gtgactcgaa
     3901 atttatattt tcgtcagaag aattgttaac atcattcgtc ttgttgtcat ttttactatt
     3961 agttggctct aatcccatat aagttccaat aacttctcta gttggtaaaa gtgagaatac
     4021 acttgaatca ccttgaggaa ttacacatac aacagctatt tctgggtcat ctgctggagc
     4081 aaaagcaaca gtccatgtaa aagaaccata atcttgttta aatctatcta tttggtcatc
     4141 tgttattttt ggatttaatt cttttatagc ttttcttaga tatgaagaat tcaccttatc
     4201 agtgtcctct aatctaactt ttactccatc ttctaattct tcctctaatt tctttctttc
     4261 ttcgtctttc aaatctttat tttctaattt cttttttatt tccttttcct tagctaatga
     4321 aagctctttt tctttttctg ctttcatctt atcagctagt ttaatcgcat catttaggtt
     4381 gacattataa gatgacatat gactttttaa atattcatac tcgttatctg taggtatttt
     4441 cccacttttc tcagctgtac cagtttttgc tgctacatct attggaaaat cagcaaaagc
     4501 actcttcgct gtaccttgtc ttgcaaccaa tttcatacct tttgttaatt cttttaagtt
     4561 atctggattt ttaaaaggta ttttttcgac tttcttctgg tcattaattt taactgatga
     4621 ataatcactt gaaacagctc tatcaactac agataattct actaaattac ctccatttgc
     4681 tatagcagct acatatcttg atatttgagc tggtgtataa gcattttcac cttgacctat
     4741 agctaagtta aatgtatctg cagtactcca ttttgcaaag ttaaaataac tgaatactgc
     4801 taaatctgct acttcttcta ttctatcttc tttaacattc attttcttta gcctattcat
     4861 tgtttcgact ctaccaggag ttttcttttc tgctgcccag cttacaatct cattgattct
     4921 tttctcatat tctttaggat ttttttctct agttatatca acaaaatcat ttgccatttc
     4981 tctttctaaa tttgacttta gaagagcttg tgttgatttc aatttatctt ctgtgctagg
     5041 aactttacct gctctttctt ctacttcatc ttgaagccct gtattttggt caagcccaaa
     5101 taattttgca tactcaagta ttttatttgc attcatatct ataccaatgc tttttccatc
     5161 tggccatgtt ttacctgtac caattgttgc catatatata ttacaagact cttgtatcgc
     5221 tttatataag tttgtcatac catggtttcc tctaccatgg ttccatacat agtcaccaaa
     5281 agatttttta cctaacatta taaccccagg gtcatttata gtaaagtttg gatccaaacc
     5341 attttctaat gctgccattg aagttagcat cttaaatgaa gaacctggct gaaattcccc
     5401 ttgtgtaacc aagtttaaaa gtggacttcc tgctagtaag tcatttggat tttttggttg
     5461 aagtgcttta taatcctctg tagaaatacc ttttgcaaat ttatttgggt cataattagg
     5521 ataacttgat gaagctaata cttctccatt tttaacatca atagctataa gagctgctga
     5581 ctgtgctttt cctgcatatg cactaattgg tttgtcccca aatttacttt taaaagtacc
     5641 tcctttagaa gcaacttcga ttatctgttt taatgcatta tctgaaactt cttgtaaatc
     5701 tttatctagt gtaagataca cagtatctcc tgatttaggt tttttagatt caatttcttt
     5761 actgattcta cccaatgcat caactttaac cattttataa ccatcagttc ctcttaatct
     5821 actttcatta ctcttttcta ctcctgcaag acctaccata tctccagttt cataaccttt
     5881 ttgaaggtat gattctattt gagtggatgg catttttcct acatatccta acatatgaga
     5941 agctaatgac ccattaggat aatatcttac tggttctact gctattgaaa caccaacaag
     6001 gtccatagca ctttcttcaa tttgagcaat cgtagtttct cctacatcct tagcaatagt
     6061 tactggatta tattgagaat atccttttga ttttatcaaa tcccttacga ccattatttt
     6121 tcttgcatct tcatcactca aacttttatc aatttctaat gcatcactat ttcttacttt
     6181 ttcaaacgct tctttggcac tcagtttagt ttcttttatc ttataactag ccaaccaatc
     6241 tctcttatct ctttctgctg tgaacatcca tttactaact aaaataggtg gataatatcc
     6301 attttcattt agttttgcct gtaatcttgt cgcatccaat cttttatctt cttgagataa
     6361 tattccagct gatataagtt tgtctactaa gtaataaaaa ctttccttag cattataatc
     6421 atttggaata ccattttctg atttatattc cctaatatct ctgtcatatg tataatagta
     6481 ctttccattt tctacatata taggaaactc atctacatat tcttcaccat ttctctccag
     6541 caaatttata agttttaatg atatttcatt tgctcttgaa tgcttatttg catctttctt
     6601 attaatatcg ttacctgaaa cttgaacagt aaataagttt ttatttcctg ccaacagtct
     6661 tccatatcta tcttttattt ctcctcttgg agcttctatt gccaactttt tatatgtttt
     6721 attctcagct aactcattgt aatattcata tttaaatgta gtcatataga ttatttttac
     6781 taaaatagct aaaaatatca cgctcatgac tatcttaatt atttttagtc tatcaacttg
     6841 tttattttct tccatgaaat caccaactaa tcttctttaa gttttaaaac tgctctttta
     6901 gataagcgat atattactaa accaattaaa ctattcacta ttggtgctac tagtagacct
     6961 tttacaaaaa gtgttgatat gttgtagctt tgataaatta tactagatgt acctatattg
     7021 acaattgaat caattaaact gcctataaaa attaaaacga aagtaataat ataactacct
     7081 ttatatagct gatctctcat gtatccaatt gcatatgcaa taataaacaa aattaatgca
     7141 tttacaccaa aaattccacc aacggttatg tctttaataa gccctattat tgctgctata
     7201 atcccacctt ctaattcatc taaataaaga gagattatgg ttacatatat aagaactaaa
     7261 ttaaagctta tgccaaaaat atctatataa tttgttatac tattttctat agtcataagc
     7321 acaatcccca acagacaaag taaaactttt ttcattgttt ttatcccctt tatcctatat
     7381 ttcttggttc tatgactaca acgtcattta tattcttaaa atcaacataa ggttttacaa
     7441 ctacatattt taatgactta tctttatcat caaccacctt ttcaacttcg ccaattggta
     7501 taccatctgg gaagaatcca agacctgatg ttgttattat atctccttga attacattat
     7561 atgatgagtc aaacatatat ccttgcaaat atcctttact attataacta tctttattat
     7621 ctaaagtagt attttgagta attgtacctt tagcattggc atcctttgct agtttaaagc
     7681 ttacagatgc cttagaatcc aatagtgaaa ttgccttact ataattgttg gaaacttcat
     7741 aaactatgcc aactaaccca ctaccattca taactatact ttcttttttt acacctgaat
     7801 tttttcctgc acctatgaca aaactctcat accagtttcc atcattttta ccaacaacac
     7861 ttgctgaaat acttgtcgcc ttatactctt cttgtacaaa gttcagtgtc ttttttaact
     7921 cttctagcga ttctgtttta tctagtttgg catttaaagc tatcatatct tttttaagtt
     7981 tttcattttc ttcttccaat ttttttacct ttttagaatt atctttaaaa tttgctacat
     8041 ctttaaaacc atttttgata aaagaaaatc catcatttat tcccttacct acacttgtaa
     8101 tagcatctga agctactcct aaatttactg gactaccact agaaaattta ccaattgata
     8161 tacccacaat tccaattaaa gtgatagcaa ctacgcccgt tgctatcact ttaacattaa
     8221 tccttttttt ctcatttttg ttcttgtcaa atctcaaggc catcaccaac ttctaattct
     8281 tagtattcat cattaatact ttttcaaaga tttcttggtc ttcaacagat ttacctgttc
     8341 caagtgcaac acaatcaaga ggtgtttctg ctatttgtac agacatacca gtttcctgtg
     8401 taataagctt atcaagacct ctaagcaaag cgcctcctcc tgttagcatt attccgtttt
     8461 ccattatatc tgaggctaat tctggtggag ttctttctag tgtagatttt attgcatcta
     8521 ctattgaact tacaggttct tttaatgctt ctctaacttc tgtagagcat atttctattg
     8581 ttttaggtaa tcctgagatt aaatctctac ctcttatttg catatttatt tcttcatcat
     8641 ctttaaatgt agaacctatg ttaattttta cattttcagc agttctttca cctatcataa
     8701 gattatattc tttttttaca taagccacta tagactcgtc aaattcgtct ccacctattc
     8761 ttatagattt agcagtaacg attcctccta aggatattac agctatttct gaagtacctc
     8821 caccaatatc aactaccata ttaccttctg gttcttcaac ttttaatcca gcccctatag
     8881 ccgctgccat tggctcttct ataaggaatg catctttagc tccagcttgt cttgcagcct
     8941 cttctatagc tctcttttct acttctgtaa ctccaaaagg aacacatata gctattcttg
     9001 gacttacaac acctttttta tcagccgctt tttgtataaa atagctaatc attgattgag
     9061 ttatgtcaaa atcagctatt actccatctt tcataggtct tatagctact atatttcctg
     9121 gtgttctacc tatcattttt ttggcttctt cacctactgc taatacctct ttactatcat
     9181 ctcttattgc gacaactgat ggttctctaa caacaatacc ttgtcctttt atgtaaacta
     9241 atgtatttgc agtccctaaa tctattccca tatcctttgt catcttatta aaactaaaaa
     9301 aacctttttt ttctttcttt ttttccttag ccatgatttt actccttcct actattaaga
     9361 atcatgttta aaacattctc ctattatttt atcacatata aagtcaaatt atcatatttt
     9421 ctttaaagct aaagtataat ccatctccaa taattatatg gtctattatt tctattccaa
     9481 ttatttctcc acatttaata agtcttgatg tgatattttt atcttctctt gatggttcta
     9541 cacttcctga ggggtgatta tgcatcacta ttatcttact tgcacttctt cttatagctt
     9601 ctttaaaaac ctctcttgga tggactaatg acgagcttaa agtccctata gatacatcta
     9661 catcagatat tatctcattt tttgtgttta aaagaactgc tttaaaaacc tcttttttta
     9721 aataccttag gctatccata taatacctct gtatatccca aggattcctt attttatact
     9781 ttataggttt aaaactagct actctagagc ctagttctaa agctgcttta atttgggttg
     9841 attttgataa cccaattcca tctatattac acagctcctc tattgtcata tcttctaaat
     9901 gtctaatacc ctgaatatct ctatttatta tatagttggc taattcaata gcatttttat
     9961 tcttgttgcc agttcttaaa agtatagcca acaactcagc atttgataag ctttttacgc
    10021 ctttggcaag catcttttct ctgggtcttt cctctggtgc catttctttt accttaatgg
    10081 ttgaattaaa tgactttttc agataaatca cactccataa aaaagattta tactaaaata
    10141 tttttttaat aggtcgccta accttgaaat tggaagacca actatattaa aataatctcc
    10201 tctaatttct tcaactaaca aagctccata accctgtata ccataggctc ctgctttgtc
    10261 aaatgactca ttagttttca gataatcctc aatatcctgc tctgataacg ttttaaattt
    10321 cacattgctg attacatagt caattacctt tttatcttcg cacaagttta ttaagctaat
    10381 acctgttatg acttgatgct ctcttccaga tagtcttttt aacatatctc ttgcacaaga
    10441 ctcatctttt ggttttccta aaatagcatt gtctaaaaca acaactgtat cagctccaat
    10501 aacaacatct tcattatgtt cagatgctac agacatactt ttttcaaaag ctaatctcat
    10561 aactaaatgt tttggtgttt caccttccaa tataatctca tcaatttcat ttttaattat
    10621 atcaaatctt acattcgtat tctctagaat ttctttcctt cttggagagg ctgatgccaa
    10681 gattatattc atttattcac ctcatttgta cgtaatttgt acctatttag tctatcacta
    10741 tattgtaaaa aattcaacag aaatgtcgaa ccttttttgt tacactattg ttatatttat
    10801 taccattaga ataaatatac ataaaataga ggtatggaaa ttattccata cctctgtttt
    10861 atgtactcat tagagatatg gaaattattc catatcctta ttttgtccat attacaaaat
    10921 tttaatagct ttctttggac atttttccat acataaatgg catcctacac atttatctgc
    10981 atctacctta tgtttttctt ttaattctcc ctctattgca tcaaacttac attgtttttt
    11041 gcacactgta catccaacac aaagttcttg gtcaatagta actttctttt tcttagttat
    11101 gtcacctgat attacttttg tagggcattt tgctacacat tgcatacacc ctacacactt
    11161 attagggtct atcttagcta ttttgtcttc aaatattatt gcatcaaatt tacaagcttt
    11221 tacacacatt ccacagccaa tacatccagc agtacatttt tccttaacag ctttaccaaa
    11281 ctctttgcta ttacactcta caactacttc ttgactttct ggttttgtga ttattatacc
    11341 cttaggacaa acttctttac attttccaca atttacacac ttttcttcat ctattactgc
    11401 tattccatca actatagaaa tagcatcaaa tttacaagca tccttacatg ttccaagacc
    11461 taaacatcca aatttacaca tcttagcacc tgagtttaaa acatttgctg ctctacaatc
    11521 tgatattcct tcatactcat atttatcttt tgcacttgaa caagttcctt tacaaataac
    11581 ttttgctact tgtttctcac ctgcttcagc acttattccc attatttctc caacttttgc
    11641 accaacatct gctccaccta cagggcaacc attaactggt gcattgcctt caactatcgc
    11701 atttgcaaga cctccacaac ctggaaatcc acaacctcca cagttggctc ctggaagtac
    11761 tcctagtata gcttccactc tctcgtctac ttctacagca aactttttag atgcgaaatc
    11821 tagtactatc ccgaagatta agcccataat tcctaaaact aatacagctg taagtatcac
    11881 cattttcacc cctctttttt atagctttat aagtcctgta aatcctaaga aagctattga
    11941 cattaaacct gctgatataa gcgttatagg gaaaccttta aatgcttctg gtatatcagc
    12001 taactctagt ctttctctta tacctgcgaa tattactatt gctaaagtaa atccagctcc
    12061 ggctccaaat ccatttatta ttgtttctac taaattataa cttttttgaa cattaactag
    12121 agctatacca agaactgcac agtttgtagt tataagaggt aaataaactc ctaatgcttg
    12181 atataaagat ggactcatct tttgaattac catttcaacg aactgaacta tagaagctat
    12241 aaccaatata aaagctattg tttgtaagaa tccagttcca gtttttacta ataaaatctg
    12301 tataaaataa gttataattg aagctagagt taaaacgaac gtaaccgcaa ctcccatccc
    12361 tactgctgta tcaaccttct tagaaactcc caagaaagga catataccca agaattgaga
    12421 tgttataacg ttattaacaa gaacgatact taaaaataaa agtattaaat tcatttatta
    12481 cacctccact atgctttttt acttcttaat ttattaaatc ctgcaaataa gaatcctaat
    12541 gttaagaatg cacctggtgg taatataaat attaatactg gttggaaaga tgctccaaat
    12601 aatgtcattc caaataaact accatttcca agtaattctc ttactgcacc tattattgta
    12661 agtgcaacag taaatccaag accttgacct acaccatcaa ctattgaagg catagctcct
    12721 gttttgaaag caaaactttc tgcacgagct aatattaaac agttaacaac tattaatggt
    12781 atatatatac caagagcctt atcaagagca ggaacatatg cttttaaaag catacctact
    12841 attgtaacaa atgtagctat aactactata aatataggta ttcttatctt atctggtgta
    12901 atttttctta ttaaagatat aactaaattt gcacatatta aaactgctgc tgttgaaaga
    12961 cccataccaa ttccatttat tgctgaagtt gtaacggcta gtgttggaca catacctata
    13021 acttgaacaa atgttgggtt ctcatctatt agcccatttt taaatacttt agctaaattc
    13081 ataattaacc ctccttattt attaagagca ctattgaaaa catctatagc agcatttact
    13141 cctgtagtaa ctgctgtaga tgtaatagtt gcacctgata ttgctaatat ttgattatct
    13201 gctgttgtac ttccttttgc tacttcaaga ggctcagcag tttttccttt gtattgaccc
    13261 ttaaatgcat catccttagc ttttgctcca agacctgctg tttctgacat attaccaatg
    13321 tcaacacctg taacttgacc ttcagatgat atacctacca taacttcaat ttctccacca
    13381 tatccacttg gtttagcttt taaagtatat ccaaccactt cagaaccatc taaaccttca
    13441 tacacttcag ctataagacc atctccaagg tctttatatg tacttttatc catttcttta
    13501 aattcttttg cttctggaag gactgctttt ctaagttcat tattttcttg tatatttctt
    13561 tgttctatta ctggagctgt aacctgatta gtagctccaa gagctaatgc agctattgca
    13621 cttatagcaa gtaaagtacc acctaatctc accatactgt tcattattta gccacctccc
    13681 caaatactct atttctaaca aacttatcta taagtggagc aagacagttc actaatagta
    13741 ttgagtaaga tacaccttct ggatatccac catacattct tataacagtt gttataagac
    13801 cagctaatac agcgtatatt atttgacctt ttttagtcat aggcgaagtt gtataatccg
    13861 taagcatgaa gaatccacct aacataagtc ctccagcaaa cagttgatat attgcgaaag
    13921 ttatattaaa tccacctaaa aggaatgtta atataaatac agttgctatg taaaatactg
    13981 gtataacata atttattatg cctttataca ttaggtatac tccacctaat aataataata
    14041 ttgctgatgt ctcacctata catccaccaa tattccctat catcatgtca gcaagactaa
    14101 ttccattgct agaaaggtct gccagtcctg ctgctccatt tttcaaaaag cttaatggag
    14161 ttgctgtagt aactgcatct aaatttttac ctatccagtt aacacctggt tgagtccaag
    14221 cagtcattgc tactgggaat gatgctaata aaaatgctct tgcagcaagt gctggattga
    14281 caaagttaca tccaattcca ccaaatacca ttttaactac taatattgca aatattcctc
    14341 ctactagaca catccaccaa ggaagtgatg ctggtacgtt aaatgctaat aataatcctg
    14401 ttacaacagc tgaaaaatct cctatagtac ttttttgctt cgtaaatttt tgatataaaa
    14461 attcagtacc tactgtacca agtatacaaa aaaacatagc actcaaagca ttaagtctaa
    14521 agaagtatac tcctgctact gctgctggaa ggagtgctat aataacttgt ttcattatat
    14581 atgaagtatc ttcattactt ctcacatgag gagaagatga tactatcaac ttattttcca
    14641 tcgtttttcc cccttaactt tctttatttc tgagcagctt gctttcttct ctttgctcct
    14701 atttctctct ttgctgatct tatagattga agtaatggtc ttcttgctgg acaaataaat
    14761 gaacaagacc cacattctat acaatccatt gctctatgat attcagctgt gtcatagtca
    14821 tcttttaaag aatatgcact tatgaaaagt ggttgtaaga atgatggaca tacatctgta
    14881 catcttccac atcttaaaca gttttgaata tctggagtac gtgattcttc ctcatccaag
    14941 cataaaattc ctgaagaacc tttgttagtc gctatttcag tagtatattg agctattccc
    15001 atcataggtc cacccattat aaccttacca atcttcttat cttctttaaa tccaccacat
    15061 tgttctatta tttctgaaac taatgttcca acttttgtta ttaggttctt tggctctttg
    15121 atgcaactac ctgttattgt agtgattctt tcaaccaaag gcatacctgt tttaatagac
    15181 ttagctattt gtgctgctgt ggcaacattg tcaacaactg cacctgctgc tattggtaat
    15241 ccaccagatg gaacttcttt gcctgtacac gcataaataa gttgtttctc tgctccttgt
    15301 ggatatttca cttgaaggcc aacaacttcg atttcactat agtctttagc tacattttga
    15361 attgcttcta tagcatctgg tttattcgtt tcaattccta taaatccttt tttgacatct
    15421 aataccttca ttaatgctct taaaccaaac acaacatctt ctggattttc aaccattaat
    15481 ctgtggtctg cagttaagta tggttcacat tctgcaccat ttaatataac tacttctgcc
    15541 ttgctatctg gtggaggaga tacttttaca tggttaggga atgtagctcc acccatacct
    15601 actattcctg cctcttttat gatacctact atatcttctt tgctcaaatc ctctaactga
    15661 ccttttggtt gaacactctc atgtaattct tccttaaagt cattttcgat aactacacat
    15721 tgagctgaac ctcctggtac ttcatgttgt tcaatagcaa taactttacc tgatactgaa
    15781 gaatgaacgt tacatgaaac aaaaccttgt tgttcaccta ttttttgccc aaactttact
    15841 tcatcaccaa cttcaacaat aggtttagct ggtgccccta tatgttgctg aagaggaatg
    15901 taaactatct ttggagcttg agctttttca agtgcctttg tgttagagta ctcttttcta
    15961 tatggaggat gtatccctcc cttaaaagtt aagagtttca ttgttttacc cccttatcta
    16021 taaaaaaaca ttttttctgc cttctataag tatactatat atattaaaaa atcttaacaa
    16081 ttgttaaaaa aatatccgta tttttataat ttattttata aatcaatacc tctatattaa
    16141 gtcaaactga ggaaaaggat tgcttaatta aacaatcctt ttcatatagc tttctaaagt
    16201 ccaaattttc tgtattttat tttatagaat ttataaacat agagtactcc tgcatggatg
    16261 ataaaagcag acttttactt acactatatt tttcttcatt tgctgctttt gaagctgtat
    16321 caattttttc attcacattc tttacatact ctattaaatt atctttaaat aaacgcattt
    16381 cagaatagtc aatcttttca gcttcttttg atatattttg tgatattgcc tttctatttg
    16441 tcaaaatagt attatatttt tccatatcta cattattttc agcattcgac caaaaacttg
    16501 attcttcttt aaagtttgtt attaatttat ttaattcttt agatatactc tctatataag
    16561 cgtagttact tgtatactct aaagacaaca taggtgcatc taagtgtgat aaaaatgcat
    16621 ctggaaacac ctttcttaca ctgcttatct gctttctaac atcattttca tcaaaagagg
    16681 aataggtctg tatttttttt actccatcct tctccataac tgaaaaaggt atgtcattat
    16741 taactaatga tgtctgtatt tttttcaaat catcattatt atctattgca gcaacttgaa
    16801 tagtgtacat atcccagcct tctatagtag ctaatttgac ttcttctggc tctttagtgt
    16861 tatttaccgc tttttctgga ttagtttctt gtttatcatc tgaattggtt tgtgatttag
    16921 attttttccc tttttttatt gaatttaatt catcagatat atctttataa gttatatctg
    16981 atgaattatt tagaaatggt attttagccg atacatactc caatatcttg ctatctttta
    17041 tttttgtata agaataatag ccaattagac acaagcaagc aatcgttata acatatttac
    17101 gtaaatcaat cttatgtcta ttattattaa atcctctgta tatcgttttt cttctattca
    17161 ttagtttgtc cctcccaaaa ttatattatt aataaagtat atttttaaga gtcacaattt
    17221 atgaatataa ataattattt cttctaattt tttaataaga aattgtactt actaaacaaa
    17281 tcttcttgca ccaagtaata ctttagacca atatgaacta gttaaattat ccactttaac
    17341 cttattagga ttagtctgag taccactaca atggataaat ttactatttc ctacatatat
    17401 acctacatga ttagttgttc catcaccatc agtgtcaaaa taaactaaat ctcctggtgc
    17461 ataattcccc atagaaattg cacttccaaa attagcctgt tctctagaaa ctctaggaat
    17521 agaaacgcct acagaatgtt taaaaacata ctgtgtaaat ccagaacagt caaatttatc
    17581 aggtcctgaa gctccccatt gatatggtat tccaataaga gtatatgcaa aatctacgat
    17641 tgttgaacct tttgctgtag atttatctga atttccagta aatgatggct ttgagttact
    17701 attagattga gaattttggt tagatgatga atttggtttt tcttttgtac cttcattagt
    17761 ctcagaaata taactggcac tcacccatcc aattgttcca ctcgataact ttaccttata
    17821 ccaaccatta tttttggatt ttaattcaac aacatctcca ccatttaatt ttcctattat
    17881 agaatagctt gtcccaggac cacttcttac atttaaactc acagcagatg ttaccttacc
    17941 attcttgcct tcaattgatt cttcgctaga tggtttttta tcagaattat tagtactatt
    18001 tgaattacta gaattattcg ttccaacatc ctctgatgtt ttagagatat attggctact
    18061 agcccatcca gtaactccat ttgatagttt tattttatac catccattgc tttgttctag
    18121 taactttact acatctccat tatttgcttt accaactaat gaatagttag tacctgcacc
    18181 acttcttaca ttcagtctag aactcacatt tactttacca tttccactaa tagttgtccc
    18241 acttgaagtt ggattatttt gatttgatgt accttcagat gaaccacttg ttgatatgta
    18301 ttttgcgctt ccccatccaa caacagaact cgataccctt actttatacc atccattaga
    18361 cttttctaaa atctctacag tttttccctt gtataatttt gctaccttat cacaatcaga
    18421 acttggtcca cttcttatat tcaaggcact tgctgttact gttccctttt caagagcact
    18481 tgcatttatg gagctaacag atactgctac tgccccaatt cctaaagctg ctatagcttt
    18541 cttcactact atcactcccc ccaacaaatt tttatagtca taaattcata acattattat
    18601 ccatttttag tccctacaat ttaatgataa cttattctct tattaattgt aaagaaatta
    18661 taaacatatc cttaaaatta atgattttta ggaagtattt caatccaata attatctggg
    18721 tccgctataa aataaattcc catactttca ttttcaaaac atatgcaatc taaatcttta
    18781 tgaagttttt ttgcctcatc aaactcctca acttctaaag ccagatgaaa ttcattgtct
    18841 cctaaattat atggtctgtc ccaatctcta agccaagtta attctaatgt atgactagta
    18901 ataccatctc ctaaataaac taatataaaa cttccatctt ctgcttcctt acgctttacc
    18961 tctttaagcc ctaaagcttt tttataaaaa tctaaacttt tttctaagtt agtaacattg
    19021 aaattgttat ggcaaaaatt aaacttcatt ttgaaatcac tctccaattt ttattttttt
    19081 tatattatat cattattata aatttattta aatcatatta agttaaaaat ttgaatgatg
    19141 atgaccaatt accaaatggt ggtaaattat ttaaaagaat atgtcaaaaa aagaataagc
    19201 tctttcaaat gcacaaaaaa tttataccca agttatactt aatcatgcaa attggcagaa
    19261 cttattcttt aaaactctaa cttttaagtt tttctttgat aatataattt attacttttt
    19321 ttctaatatc ttcagcatta taatcatctt ctacttctat atccaaaagt tttgctattg
    19381 acctcatatc ttctctagtc tttgacctat agcttgatat taattcatct tttattttct
    19441 ctggttctaa aagatattct tcatctcctc ttttgaaaac aacgtatttt tgagcatatg
    19501 aacttctatt atagtatata tatcttgata tttttcttaa aatttgatta tatgaaagtc
    19561 gcttgttaat atcaagtttt aagtactctg aaaaagcaaa tagctctttt tttgatttaa
    19621 ataatgcttc tttctttata gtccttgata ttgaagttac actttcttga tatgcacact
    19681 tttttaacat ttttaaatat actctattaa gaccaatact ttgattgaat ttttttatat
    19741 tattattaat aatcttatcc aagttacctt caaagtattt ttttgctcct ttttgtgata
    19801 cataactctc aagttctttg tgggccatat caagtatcat tttttttaaa tcctctttat
    19861 atcccctaga ttccatgata ctccctccct tagctattta tagtatattc aaaaagctca
    19921 agaaatgtga gtcattacaa aattttttta ctttttatgt atatattaat ttaaaagggg
    19981 agataatagt aatgtaaaat caaacatact caatctcccc catttttaga cctctattac
    20041 tcccccaata gaggcctttt tttagtttat caatttctat tcaattccct ttagttcaaa
    20101 tcctaattct gctatcactt cttttaattt ttcaatagat atatcatctt tcatatctac
    20161 agatgcacac ttattttcta aatccacatc taaaacttca ataccatcta tatcttctgt
    20221 taatgcagtt ttaagatggt ttacacaatg accacaactc ataccttcta ctaatagttt
    20281 cttcttcatt taattacctc cttatacccc ataagggtat tctatcatat agtttatact
    20341 ttttaaatag aaattaatca tttaatttga ttcattcgat tttatatcta aattagatat
    20401 tccctcatat acatcaaaat catttggagt aattttacct tttaatctac tattacttac
    20461 aatatagttg tttctcacag ctaaacttat aaatgttgca ttatctttta tatatgtctg
    20521 tacatctttg tatgcttttt ttgtttcaat ttcagacgta gaacttgtaa gtgcagacat
    20581 atatccattt agcttatcat cagtatatcc actacttgct ataatactac ttgcatctgg
    20641 tataattgat agtttccatc caactaatgc taaatcataa tttttagaat taagagcatt
    20701 ttccatatcc ttatctgaca gctgatttac tgtactattt attcctatag cttttaagtt
    20761 ttcatttatt atgtttgcag tttttattct ctcactatta tctctgttta ctattatctt
    20821 taagttcatt tcagaaagac ttttctttac ttcctctttt tctctatttt ttttcttttc
    20881 agcttctttt ttgattctat cttcttctgc ttttttagct tttgcttctt cttctggcgt
    20941 aagcttcttt gagttctttt tagctgattt tttctgattt tttactttat tgtcattttg
    21001 atttactgag tttatatttg catattctac cttagcaaga cactctatcg ccttatcttt
    21061 attaaactct aaatccttca tttcactatt atagtatttt gattttgaat taagtggaaa
    21121 attaactgga gtagcatcat ccatataacc ttcactaata attctatctt tatctataga
    21181 gtgagcaatt gcttttctaa aattaacatc tttaaaaaat ggattattat aattaaataa
    21241 tacacactca taatctcttc cttgatattt tgttatatta aactcttttt cttgaaactt
    21301 gcttaaatca tttaatgtaa cattggttat atcactatca agagccataa ccatagatgt
    21361 ccttgcctct tcattaggca ccatacctac ttttatgttc ttcatggtct ttggaacttc
    21421 ttcatagtaa tctttgttta cagataaaac cattccttct ctttctgtat atgattctat
    21481 tttatatttc ccatttccta tcatattatt attcttgtca tttacatcct ttgaatctaa
    21541 atgattttga gatacgattg gaaaaattaa actatcaatt gaaaaagcat atctagcttt
    21601 aaattttatt gtaaaatctt tatctgattt aatattcaca gaagctatat ttttagtaaa
    21661 ttcattatat ggactatctg cacttttttg aattaaacca atagtatagt ttacatcatt
    21721 tgaagtaaca ttttttccat catgccactt tgcatctttt agctttatat ctatactcat
    21781 tccatcctga gctatattat actccttaac taattgagga attacattgt agttctcatc
    21841 tatagtaaat aaactatcat aaactaaatt cattatataa ccaacagact tatcagtatt
    21901 agttatagga tttatagttt ttggagttac cattgttaag tttatatact tagcatctat
    21961 agaactttga ctttgtaaac ttgaatcact actactttgt tttgttttag tattactaca
    22021 accagctacc ataagagtta tagctaaaac gactgttaag acttttattc tcttcattag
    22081 ttttttcctt tcttgccata tatcttatta taatgttcgt aattattttt tactttttct
    22141 acatattttg aagtttgctt aaaaggtata tcatgtagat tttcgccatc tttactatat
    22201 tcattatttt ctaaccattt tttcacatta cctgaaccac cattatatgc agctatcact
    22261 aaatctaatt taccaaattc tttgtataac ttacttaaat accaacagcc aagtcttata
    22321 ttagtctctg ggtcgaaaat atccacattt tttaaattta gttcctctgc tccccaatct
    22381 ctagttatat ctaatatttg cattaatcct tttgcttcct tttttgaaac agcagaacta
    22441 ttaaacttac tttcggcttt aataacactg taaactatat tttcatctaa attaaattct
    22501 tttgaatact tttctacata ctctgaatat tttttaggat ataaaaattt atgtattact
    22561 ttgctttcca ttaatagtgc cccaaataag attataaaaa tagatagaat caatactttc
    22621 ttactattca cgccaattct ccttcatgta ctcaataaat ttatgtgctt tactttctaa
    22681 ttcagttatt gttcccgaat tatctattat ataatcacca tatttggatt tttcttcctg
    22741 actcatctgt gaatttatcc tactaagtgc ttcctgctta ctacaattat ctctcttttg
    22801 tattctacta atttgtactt cttgtttgca agtaactact aatagcttgt caaccatatc
    22861 taaaaaacca ctttctacca atattgctgc atctaatact actatatttt cgcctttttt
    22921 tctcaattct tctatttcac ttattatttt ttctcttatt cgcggatgag tcaagctatt
    22981 taattcctta agtttttctt catcgctaaa aactattttc ccaagagctt ttctatttag
    23041 agttccatca tcatttttta tactttgccc aaaatggaca aatacttttt ccaataacaa
    23101 cttatcctca aatatcttcc tggatataat atcagcatcc actatagaaa tattaagatt
    23161 tctaaatata tttgataggc tgctttttcc acagcctatt cctcctgtaa gtcctaatat
    23221 caacataaaa tcacctactt tgtctcatac caagaactac ctacatttaa atctacatct
    23281 aagtgaacat ccatatttac agcattttcc atttcttctt ttactatttt cttaactata
    23341 tctatttcat catcaattgc ttcaactatc aactcgtcgt gaacttgtag tataagtttt
    23401 gatttcaaat tgttctcttc caatttttta tgaacattta tcatagcaat ttttattata
    23461 tctgctgcac ttccttgtat tggtgcattc attgcaaatc tctttcctct atttctttcc
    23521 ataaaattgc ttgactttat ttctggtata tatcttcttc tgtttaaaat tgttacagca
    23581 tatccatcct cactagcctt ttctactgtt gtgtccataa actcttttat tttatggtat
    23641 ttagcaaaat aactttcgat atattctttc gcctttggaa caggtatatt taaatcatca
    23701 gctagaccaa agtcactttt accatatata ataccaaagt ttacagcttt agctgcacct
    23761 ctcaattcac tagtaacatc ttccatagga acattaaaaa cttgtgaagc tgttttggta
    23821 tgaatatctt catgatgttt aaatgcttct atcatatttt catcctgact catatgtgca
    23881 agaactctta gctctacttg tgagtaatcg gcatcaacca acttacagtt atcatctgca
    23941 ataaacactt ttcttatttt tctacccatt tccattttta taggtatatt ttgaagattt
    24001 ggttctgtag atgaaattct tcctgtagtc gttatagttt gattaaatga tgaatgtatt
    24061 ctattacttt ctggatttat tatatttaaa agtccttcaa cgtaagtaga atttaacttt
    24121 actatttgtc tatactcagt tatcttatct attattggat gcttatctct taatttttct
    24181 aaaacatctg cattagttga atatccagtt ttagtttttt taattattgg aagctctaat
    24241 ttttcaaata atataacacc caattgtttt ggtgaattta tattgaatgg ctctcctgat
    24301 aattcataaa tttccttttc tagtgtagct attatttctt taaactctac actcaattca
    24361 tccaatatac ttttgtctac ttttatacct tcatactcca tatgacctaa gacttctact
    24421 aaaggcatct ctacatgata aaaaagacca tccatactca tatccattag acttctttcc
    24481 atcataggaa ttgtttcttt aactgtgcat attatttgac ccatatactc tgctaaatcc
    24541 tcaaattcta aatcctcata gtttttagat ttaacaccct ttcctaataa ttcttctttt
    24601 gatttaacct tttttgataa atacttcata gctattgcac tacaatcata tgaggtagat
    24661 gaagttgaat ctattaagta ctcagctatt gttatatcaa aatacatatt ttctaagttt
    24721 atattatatg gttttaaaga tatataatca tcttttaaat tatatccata ctttttaatt
    24781 tcattatttg aaaatatact atctaacttt gacacttcat ttgaatctac ataatatatt
    24841 ttttctccat ctacacttat aaaaatatat attatatttt tttcaagtat attaccttgt
    24901 cctctaacag tttttaaaat aacttgtcct tttttgttta cttcatctat aaatttttct
    24961 gtatctttta attttaatat ttctattttt tcttctacgt gttctgtgtc atctgaatcg
    25021 accaaatcaa ctaatcttga taagagactt gtaaatccaa agtatctaaa tttatctaaa
    25081 agttcatctt tatcataatc gccaaaaacc atactatcta aatccacttc tattggcaca
    25141 tctcttataa ttgttgcaag tcttttactt tgaatagcta tttctttatt ttcctctatt
    25201 ttctttttta cactaccttt aagcttatct gtattctcta ttaaattctc tatactagaa
    25261 aactctttta taagctttat acctgttttt tcaccgattc caggtactcc aggtatatta
    25321 tctgacttat ctcccataag accttttaaa tcaataaatt gtgctggagt catctcgtat
    25381 ttttcaatta cagaattaaa gttgtattct tcaacttctc caactccttt ttttgttata
    25441 agagtagttg tattatctga agcaagttga atagcatctt tgtctccagt tactatgtat
    25501 accttatacc cattattttc tgctttttta gaaacagttc ctataatgtc gtctgcctca
    25561 tacccatcta tttccaatct atttattttg aatttatcta aaaggtcttt taatggctgt
    25621 aattgttctg ccagttcatc tggcattccc tttcttccag ccttatactc tttatattct
    25681 aaatgtctaa atgtaggtgc ttttctgtca aaggctacac ttatatgtgt tggtttatat
    25741 atatctatca ttttaaatag cattgttgta aatccatata ttgcattggt ctttaaccct
    25801 tctttattat tcatttctgg cagagcataa aaggctctgt ttattattga atttccatct
    25861 attataatta attttttttc caaattatat cactcctaaa aattatctat ttttgtaaca
    25921 tatatattat ataccaatat agtgttattt aatattccca taataataat agtatattaa
    25981 aattaaatat gttgcaaaca atatattttt ttgctataat aatatacatg acatgcccga
    26041 atggttgaat tggtaaatac aatggattca aaatcaactg tgttgattca taagttttta
    26101 taatgtttta cagtttgatg agtattttgt attcaatatt cctcttgcat attcttggaa
    26161 aatctggtag gatatacaag tagtttggat ttaaaatata agccgctgtg gcggaactgg
    26221 catacgcata cgactcaaaa tcgtacggga aaccatatgg gttcgagtcc catcagcggc
    26281 accaataaaa aaaactcttt agtatatact aaagagtttt tttattgtaa tattctttca
    26341 tctttatttc tagccttgta taagtataaa taccatagtc ataacttctt gaactcctaa
    26401 aatatatcta aacaaagtag gattttttat gtattcactt aataaatcca tacttcttat
    26461 catattgatt ttttcccaat ctctaaaaat tttgatacaa ataaaaaata cagagaacta
    26521 cttgatatac tagaaggtta ttctgtacca tttggtattg ctgtaggtgt tccagatgga
    26581 aatttaccaa atgctagaga tgtagtttat aaagtttcta gagtttagga taaagtgttg
    26641 caacaatcaa ttagttactt gaagagttaa atcagtaata tacaaataaa ttaatggaag
    26701 aggaagctat ttaaaaagta gtttagattt ttcattgtaa aataattaaa taaaga
//
LOCUS       DAELXX010000052        26635 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.052, whole genome shotgun sequence.
ACCESSION   DAELXX010000052 DAELXX010000000
VERSION     DAELXX010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26635
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.052"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(69..1709)
                     /gene="bclA2"
                     /locus_tag="KPH29_002022"
     CDS             complement(69..1709)
                     /gene="bclA2"
                     /locus_tag="KPH29_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HBG1467615.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGATGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGANGTTGSTGPTGNTGATGANGITGPTGATGATGA
                     NGITGPTGNKGATGANGITGPTGATGATGANGITGPTGNTGATGANGATGLTGATGAT
                     GANGITGPTGATGATGANGVTGATGPTGNTGATGPTGSIGATGANGVTGATGPIGATG
                     PTGAVGATGPDGLVGPTGPTGPTGATGANGLVGPTGPTGATGANGLVGPTGATGATGV
                     AGAIGPTGAVGATGPTGADGAVGPTGATGATGANGATGPTGAVGATGANGVAGPIGPT
                     GPTGANGVAGATGATGATGANGATGPTGAVGATGANGVAGPIGPTGPTGANGTTGATG
                     ATGATGANGATGPTGATGATGVLAANNAQFTVSSSSLGNNTLVTFNSSFINGTNITFP
                     TSSTINLAVGGIYNVSFGIRAILSLAGFMSITTNFNGVAQNNFIAKAVNTLTSSDVSV
                     SLSFLVDARAAAVTLSFTFGSGTTGTSPAGYVSVYRIQ"
     gene            complement(2087..2626)
                     /gene="hpt"
                     /locus_tag="KPH29_002023"
     CDS             complement(2087..2626)
                     /gene="hpt"
                     /locus_tag="KPH29_002023"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBG1467616.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGKVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            complement(2954..4261)
                     /locus_tag="KPH29_002024"
     CDS             complement(2954..4261)
                     /locus_tag="KPH29_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBG1467617.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDAAEKAESLMDTVTIQELIKNVEEIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDEKLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            complement(4686..6461)
                     /locus_tag="KPH29_002025"
     CDS             complement(4686..6461)
                     /locus_tag="KPH29_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG1467618.1"
                     /translation="MMVMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDVCRECYKSGACVEFAEVCCPIICD
                     GDSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLEKMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            complement(6458..6595)
                     /locus_tag="KPH29_002026"
     CDS             complement(6458..6595)
                     /locus_tag="KPH29_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467619.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            complement(6673..7242)
                     /locus_tag="KPH29_002027"
     CDS             complement(6673..7242)
                     /locus_tag="KPH29_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG1467620.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(7426..8061)
                     /locus_tag="KPH29_002028"
     CDS             complement(7426..8061)
                     /locus_tag="KPH29_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG1467621.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
     gene            complement(8729..9610)
                     /locus_tag="KPH29_002029"
     CDS             complement(8729..9610)
                     /locus_tag="KPH29_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBG1467622.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGVKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
     gene            complement(9730..10737)
                     /locus_tag="KPH29_002030"
     CDS             complement(9730..10737)
                     /locus_tag="KPH29_002030"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HBG1467623.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            complement(10757..11239)
                     /locus_tag="KPH29_002031"
     CDS             complement(10757..11239)
                     /locus_tag="KPH29_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBG1467624.1"
                     /translation="MSFVGISTSMKNTSLHIFRDPLLDLIDNDSDIDLLGVVVVGTSE
                     HNEWKTFLATRLGRWIESLRPDGVIITLDMAGNQHIDFTNAIAEFVKSDIPAVGLTIM
                     GADGLVITNPYLDKATIIDYKKTTGYIETEVVGDNHMDEVDVKKALAFLKLKMRKDAK
                     "
     gene            complement(11257..11724)
                     /locus_tag="KPH29_002032"
     CDS             complement(11257..11724)
                     /locus_tag="KPH29_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBG1467625.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            complement(11739..12497)
                     /gene="prdD"
                     /locus_tag="KPH29_002033"
     CDS             complement(11739..12497)
                     /gene="prdD"
                     /locus_tag="KPH29_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HBG1467626.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            complement(12577..13302)
                     /gene="prdB"
                     /locus_tag="KPH29_002034"
     CDS             complement(12577..13302)
                     /gene="prdB"
                     /locus_tag="KPH29_002034"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(12850..12852),aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HBG1467627.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            complement(13340..13624)
                     /locus_tag="KPH29_002035"
     CDS             complement(13340..13624)
                     /locus_tag="KPH29_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467628.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            complement(13705..15585)
                     /gene="prdA"
                     /locus_tag="KPH29_002036"
     CDS             complement(13705..15585)
                     /gene="prdA"
                     /locus_tag="KPH29_002036"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HBG1467629.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            complement(16014..17774)
                     /gene="prdR"
                     /locus_tag="KPH29_002037"
     CDS             complement(16014..17774)
                     /gene="prdR"
                     /locus_tag="KPH29_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HBG1467630.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
     gene            complement(18244..18406)
                     /locus_tag="KPH29_002038"
                     /pseudo
     CDS             complement(18244..18406)
                     /locus_tag="KPH29_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020315.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(18575..19888)
                     /gene="prdC"
                     /locus_tag="KPH29_002039"
     CDS             complement(18575..19888)
                     /gene="prdC"
                     /locus_tag="KPH29_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HBG1467631.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     gene            complement(20277..21041)
                     /locus_tag="KPH29_002040"
     CDS             complement(20277..21041)
                     /locus_tag="KPH29_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG1467632.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKYDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKKTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            21332..21535
                     /locus_tag="KPH29_002041"
     CDS             21332..21535
                     /locus_tag="KPH29_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBG1467633.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            21725..22261
                     /locus_tag="KPH29_002042"
     CDS             21725..22261
                     /locus_tag="KPH29_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HBG1467634.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
     gene            complement(22265..23131)
                     /locus_tag="KPH29_002043"
     CDS             complement(22265..23131)
                     /locus_tag="KPH29_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HBG1467635.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            23279..23524
                     /locus_tag="KPH29_002044"
     CDS             23279..23524
                     /locus_tag="KPH29_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBG1467636.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            complement(23638..24936)
                     /locus_tag="KPH29_002045"
     CDS             complement(23638..24936)
                     /locus_tag="KPH29_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBG1467637.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLPHFNKVITTTPNNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(25069..26100)
                     /locus_tag="KPH29_002046"
     CDS             complement(25069..26100)
                     /locus_tag="KPH29_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBG1467638.1"
                     /translation="MENICDMNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYI
                     FISDKFDDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNI
                     RLINLSLSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFT
                     LDGDDSFWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEK
                     PGITISDLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKENALLMKVADVVRDCL
                     DIEDNNEKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLT
                     ELVERYKNL"
BASE COUNT         8027 a         4997 c         3010 g        10601 t
ORIGIN      
        1 atattatcct atttattggt atatgaaatc atattgttta ttattttata atcttttcat
       61 catataaact attgtattct ataaactgat acatacccag ctggagaagt acctgtcgtg
      121 cctgaaccaa atgtaaagct taaagtaaca gctgctgctc tagcatcaac taaaaagctt
      181 aaacttacac ttacatctga tgaagtaagc gtatttactg cttttgcaat aaagttattt
      241 tgggctactc cattaaagtt agtagtaatt gacataaatc ctgcaagtga aagtatggca
      301 cgtataccga aagatacatt gtatatccct ccaactgcaa gatttatagt actacttgtt
      361 ggaaaagtta tattagttcc atttataaat gatgaattaa atgtcactaa tgtattattc
      421 cctaaacttg aagaagatac tgtaaattgt gcattgtttg ctgctaacac tcctgttgct
      481 ccggtcgctc ctgttggacc tgttgctcca tttgctcccg ttgctccggt cgcccctgtt
      541 gctccggtcg ttccatttgc tcctgttggt cctgttggac ctattggacc tgctactcca
      601 tttgctcccg ttgctcctac tgctcctgtt gggcctgttg ctccatttgc ccctgttgct
      661 ccggtcgctc ctgttgctcc tgctactcca tttgctccgg ttggacctgt tggacctatt
      721 ggacctgcta ctccattcgc tccagttgct cctactgctc ccgttgggcc tgttgctcca
      781 tttgcccctg ttgctccggt tgctccggtt ggacctactg ctccatctgc tcccgttggg
      841 cctgtcgctc ctactgctcc ggttggacct attgccccag ctactcctgt tgctccggtc
      901 gctcctgttg gacctaccaa accatttgct ccggttgctc cggttgggcc tgttggacct
      961 accaaaccat ttgctccggt tgctccggtt gggcctgttg ggcctgttgg acctaccaaa
     1021 ccatctggac ctgttgctcc tactgctccg gttggacctg ttgctcctat tggacctgtg
     1081 gcacctgtta ctccatttgc tcccgttgct cctatacttc cggttggacc tgttgctcct
     1141 gtatttcctg ttgggcctgt agcacctgtt actccatttg ctcccgttgc tcctgttgct
     1201 cctgttggac ctgttatccc attcgctccc gttgccccag ttgctccggt tagtccggtt
     1261 gcaccatttg ctcccgttgc tcctgtattt cctgttggac ctgttattcc atttgctccc
     1321 gttgctcctg ttgctcctgt tggacctgtt atcccattcg ctcccgttgc tcccttgttt
     1381 ccggttggac ctgttattcc atttgctccc gttgctcctg ttgctcctgt tggacctgtt
     1441 attccatttg ctccagtcgc tcctgtattt cctgttggtc ctgtagaacc tgttgttcca
     1501 tttgctccag tcgctcccat atttcctgtt ggtcctgtta ttccattcgc cccggttgct
     1561 cccgtatttc ctgttggtcc tgttattcca tttgctccgg tcgctccggt tgcgcctcta
     1621 ggccccgtcg gtcctgttgg accagtagca cctgttggcc ctgttggacc tacatcttga
     1681 tataaacttg gacctgaaat atcactcata taattaatcc tcctttttta aagttagagt
     1741 attactctat aaatattgag tattactcta tataattata tgaatcgaca atttcttatg
     1801 tgataatgta tctatttgct tatatcaaca tatatgcttt gattcttaaa tttcattact
     1861 tttttgtaaa atcttttaca attattagta ctcttttaca attattaata caattaaaaa
     1921 actcatttcc catcaattat ttaaaatttt tttattatgt tataaaaaat agataattaa
     1981 attgcataaa attaatttaa tactacttta attacttttt aaataaaaaa acctcaacac
     2041 gattaataaa aatcatatta aggcatccac aaaattatat aattgcttat ctatcttcat
     2101 cagtaacatt gaatatataa ggaacatttc tataataatc accaaaatca aacccatatc
     2161 caaccacaaa cttatcttct atttcaaatc cacaataatc tggcactaaa tcaacttttc
     2221 ttctgcttgg tttatccaaa agaacacagc attttaaact agcaggattt ttagctttta
     2281 gatgttttaa cacaaaatcc atagtaagtg cagaatcagt aatatcgtct acaaccaaaa
     2341 catcatatcc tgctatatca gtagtcacat ctgaaaccac tttaaccttt ccagtacttt
     2401 cttcattgtt tccataacta gatgtagtca taaaattaac tctaagtggt aaatttatat
     2461 ttctaactaa gtctgcacaa aatataaagc taccttttaa tagagaaact accataagat
     2521 ttttatcttt ataatctttt tctataattg ctcctaattc ccttaattta tctttaattt
     2581 tttcttctga atacaatact tcccattttt ttgtttctat atccataaaa agacctccaa
     2641 ttgtaataat ccttcttaat tttataagag attgctcact ttttcaatac tcttattcaa
     2701 tattagtcaa tattattgag tttttatgtt cattcaacaa ttattaacaa tgtatcttat
     2761 attttaagta gttcatatat ccacactaaa ttacatacta tatattttag acaaaaaaag
     2821 aaaaaacttg cactaacaag ttttttctat tatacataaa tttatacata atcatatttt
     2881 attagagggt actaattaaa tttatttatg ctatttttgt tatacctcat aatacaaaac
     2941 atattataaa gtactagtca actttattca tatcattcat tacatttaag attatctcat
     3001 ctgttataga catacctttg tcacttactc tacctaagtt ttgtatactt tgctcaacac
     3061 ttgaaccaac tataccattt agtggtggta caaaacaatc ttgcttagct attatagcac
     3121 tttgtatagc tgcagatgct gcagaagata attttaatgc acaaccagct ttcgcaccgt
     3181 cacatatcat tccacttaag tcagctatca tattttctat agctccttct atttgctttt
     3241 cagtaccatt cataagccaa gagataccag cagttgctcc agtagaagca gcaacaccac
     3301 atccacacac agcagataat cttcctgtat agttcttaat ataaccagtt actagatgag
     3361 atatagctaa agcctttgct agtttttcat cagattgtgg gaatttttta ttataagcaa
     3421 cgattggaag aattgctgtt aatccatgat ttccacttcc atttgaactc ataacaggca
     3481 tttttacgcc tgccattctt gcatctgaag ctccagcagt taacatcatt gcataattta
     3541 taacatcatt acctaaaaga ccttcttcta tagacttttt aattccatat ccgacaccaa
     3601 taccagtttt ttgttttaat ccatattcag ccatctcttc attcattttt actccatcta
     3661 gtaaaaattc tatatcttta aaatcaattt cttctacatt ttttattaat tcttgtatag
     3721 ttactgtatc cattaaactt tcagcttttt ctgccgcatc atttgaagct gatacttttg
     3781 gttcattgtc taataatact tctccatctt tttctaagaa cgtaaaatta tcatgtttag
     3841 ttcttattct cactttagca atatgatttt ttccttttaa cacaacttct ataaatactt
     3901 tctctcttgt atcagcaggt gttattgata gtggagtatt atccatataa tcctcagcta
     3961 ttttaacttc ttcttttgtt agagtttcta acacacttaa tccattttca gaatggccac
     4021 ctactatacc tagagctgct gcaattttta atcctaatct ttcagtacct ggtataccta
     4081 cgcacatgcc atttttgtag atactaggac taactaacat acgattttca actatttctt
     4141 ctcctaataa ttcctttgcc tttgcacatg ctagagctaa ggctactggt tcagtacacc
     4201 ctactgctgg tttaacttct gcctttaaca tttccactaa ttcttttctt atatctctca
     4261 ttatatataa acctcccaat taagtttaga tttagtactc aaataattaa ttaaataatt
     4321 tcttatctat atttatagca aaatacatgc catatttttt atatcttttt atatttttag
     4381 tcttatattc tataaccatt ggtatgactg cttctattta tgtatttttt ttatttacat
     4441 agagtattta tattctttaa aatggagaaa catatgacaa tttcgcaaaa agacaaaaaa
     4501 aagtctcaga atgagattct atatcatttt gagacggctc aaatgtgtta ttttttaact
     4561 tataaaaacc attttttaat tcataaaaaa agattatgca aaacacttat agtataagtt
     4621 taaagctttc tttaaagtta tgaatttaga aaaatttaaa ttttatatag ctttttatta
     4681 tattactatg aaattatatt gtatttttca atttttctat ataatgtagc tcttgatatt
     4741 ccaagtgctt tagctgttaa ttctttatct tttttataat ctttgtactt atttaaagct
     4801 tttattatct caatcttctc tagttcatca agtggaatta tcctatcgct tttaacttct
     4861 aactcttcta cagaggtatt cttaattttt tgaggaatta cattcattgt gattattgaa
     4921 gaagaagaca tattcacact atattcaata atattctgca actctcttat attccctggc
     4981 cacttatatt ccatcatagc atcaacaacc ctgctatcta tgtcaataac atttttatct
     5041 aatttttttg aatactcttt aaccatatgc ttgattataa gtgggatatc ttcttttctc
     5101 tctcttaaac ttggtaattt tataggtata acatttaatc tataatataa atcttctcta
     5161 aaagtgccat tttgaaccat tttttctaaa tctttatttg tagcagcaat aattcttaca
     5221 tctattgaga catttgattt acttccaatt ttattaagtt ccttttcttg taatactctc
     5281 aataactttg actgtaaatg caaactcata tctcctattt catctaaaaa tatacttccc
     5341 ttatgagcta cttcaaacat tccaagcttt ccaccttttt tagctccagt aaaagctccc
     5401 tcttcatatc caaataattc actctctaat aaattgtctg gtatagcagc acagtttaca
     5461 gctataaaag gattatctac tctattgcta tggttgtgta tggctcttgc aaatagctcc
     5521 ttacccgttc cactttcccc agtaataagt actgttgatg ttgatttaga agctattagg
     5581 gcttcctttt tagaatttct tatagctaca ctttctccaa ttatgttttc caattttatt
     5641 ctataatctt tattcatctt attgtagttt tttatagcat ctcttttatc tataaaatca
     5701 ataacatatc cctttaactc attttttaat actattttat tgatgttgta tattcccttc
     5761 gaatcatatc cgtatctttt ataaaaaaaa ctacacgatt tgtctttctt aaagttattt
     5821 atagaaggtt tctttataaa atctaattcg acaaatatgt ttttgccagt tatactatcc
     5881 tttatattga aaagctcttt aaattttgaa ttatatttat caatttttcc atccacatca
     5941 atagacacaa cagctttgtc catattgttt aatagtgtct ctaatttttt cttttctaat
     6001 tcaagctcat aagttttaat ttgtgctttt aatttattag atattaagtc tgccattttg
     6061 cctaaaaagt ttataagacc acttttatta ttttctataa tttgtgcttg ttcttttgta
     6121 aaggctataa ggcctataac gccatgagaa tcgccatcac atataatagg acaacacacc
     6181 tctgcaaact ctacacaagc cccactttta tagcattccc tacatacatc actactcttt
     6241 gggtcatcaa ttagtattgt gatctgttct gtaagtgatt ttttaaaagc cgtatattta
     6301 tctactttct cacctatttt gtctatatat actcctgtac ctgcaatcct aaataactcc
     6361 ttatttacta tagttacatc tatattgagt actgatacta tagcttcagc tatattttga
     6421 atatcagatt ttatatattc taaacctatc ataaccatca ttccttattt ttctttaaca
     6481 taagtatttg tcttatttct ttaagaatca agtatatctt atttaaagta aattggattg
     6541 acatcaggat tacaaagagc ataacaatta ttattaactc ataatttatt tccatctaaa
     6601 catctcctta taacaaaaga ctaggcttaa cctagtcttt tatattatat ccatatttat
     6661 attttaactc atttatcttc tttttcccac ataaatatat tttaatttat ttgactcttt
     6721 aatcatacca tcaaagttta atcttgaaaa tatattaaat acttcatgtg gatagtgaat
     6781 ctttgcaaca gaaccacaaa atttatgttt aagataattt aacttaactt ttaaattctt
     6841 taaatttaaa tcctctaata taacctctcc attgcccttt aacactcttt gtatttcaga
     6901 tgaagctttt tcacaatctg taataacatt aaattggtca actattatta tcttgttgaa
     6961 ctgattatca tcaaatggta gaaactctaa cttaccttta actacattgc aattttcatt
     7021 atacactaga gaataacaga catcttcata attactattc tccagtatag ttacagaagt
     7081 tccaagacct ctcaatctct tgcctaggtt tcctatatga ccaacaagca atattttatc
     7141 acctacatta tactcaattg tatccaaaaa acattctaat tcatcagatg tatatttagc
     7201 tttaactggc tgtttattta tctgacataa tatgcttttc acaatgcttc acctcttcat
     7261 ttcctcaatt ataaattatg tacagattac attataataa atggcgagca actattcaag
     7321 agatatttca ttagtatttt tttaaacaat ctttatacat tttaatatga tattatacca
     7381 aatttattag gattttattc ataaaaatca gtctattttt tatgtttatt cattgttatt
     7441 ttcatccaat ttttttattt tcgatatgga tacttcatat gccatttttt tgaccacatt
     7501 attttcatct atttttttct catattctct actttgaacc ctaccccaaa gctgtattct
     7561 atcaccaacc tttagattct ttgcaaattt agcatttcta ccccaaacta ttgatggtat
     7621 ataatcagat ttattataag gtctgtttat agcaactaat aaatcggtta tttcccttcc
     7681 taaaggtgtc ttcctataca caggttcctt acatacatat ccatctaaaa atatactatt
     7741 agggtcttta ttttcctcgt ctatgctttt tatttctctg acaaacactg taagcactaa
     7801 tctattttta ttatttaatg ttttgttata tgatcttaat tggcctacga cattgactaa
     7861 attttgttta tcaaggtcaa tatcttggag aagtctttct gatacagtca ttggaagtat
     7921 atcaaaagaa tcgcttagtc tgtttatttt tattttcata ttataaaatt tttctccaaa
     7981 tatctcatgg ctaaattcta gcttattgtc taactcccct cttaagttta ctacgttatt
     8041 ctcatcttta acagctatca tattttatcc ctccacaatt gttaattctt atatttctgg
     8101 tatcagctta tataataaat atatttataa gggatgtatt ttattacaaa tctttgattg
     8161 ctgtttaaaa gtttttattt agttataaat ttcatttaat atttcacttt tataattata
     8221 aaattattat ctttttgtac tacatatact aattaaacta cctatttcag ttttgtatta
     8281 attataaaaa tcctattata taattaataa gtcatatata acttaaattt attcactcta
     8341 tataagcaac ttcaacgaat taaatttatt caaataattc agatgaatat tgagtcatta
     8401 taaacttatt tttcttaatt ataagttata ttagacttat tacagcttat atttttatta
     8461 atctgtatta atatatgaac atttattgaa cttattgatt ataaaattta aatatttagc
     8521 tttaaattat gctttattct ttgaaaatac aacgtttttt acattaaatc tactaaatca
     8581 ctatatatat tacataattg gcatgttaat tgctttatat taatttagca aataaaaaac
     8641 tgccctaaaa tattaattca atattttaaa gcagtttata atctttatta ttagctgtaa
     8701 tatttaataa acactaagta gtctttaact aagcagtctt tttgttttta tttgcagcca
     8761 ttatcattgt agcagaagta taagctataa ctactattac taatgctttt aatatatcca
     8821 ttggtagagt ttttactata tatgcagcta cgaaaactcc tattaaacca catatagcta
     8881 ttataactga tgctttttta gtatatgcat cttcttttat aaatttcata cttgcaactg
     8941 gcattaataa tgcacaagaa cccataacta ttgggaatgc aacttttgca gacattccta
     9001 agaaataaac cattgccata cctggagcat atagacctat accagctgtc attaaagctc
     9061 ctagtataaa gttacctatt acacctatta ccaatttaac accagtaaga cctaattcat
     9121 ttcctccacc tggtaatacg ttaatccaag gatgacctaa taacattaat attgctgtta
     9181 atgctaaagt tacacccata acaagttgta tttttctctc gtccatctta gctattattc
     9241 ctgcacctat atatgaacca actgctgctg ccacaaccat agatactaaa gtaacaccat
     9301 caactggtat gattgatgta aatatgaatg cttctgtaac aactggaata gtatgtgcta
     9361 cgtttaatgt accaggcatt tttttatctg gtatatttaa ctttaaagcc tttgacattg
     9421 ctattgttgg agcaaatgac cctattccta atgtatcaaa gaagtctgtt ataaatcctg
     9481 ttacagcaac ttttggagta ctttgttctt ctagtttccc ctctttgttt gctttagcta
     9541 cgtctttacc aaacacaact gcaaagattg ctgcgaatac tacatataat gcctttaaga
     9601 ttgctaccat aatccatcct cctatttttt tattttttca tatattctga atcttatagc
     9661 ctaaaagcta tagaatagca accgacaagt ggctactatt ctattttaat ttcctaattt
     9721 atttattact tatttaagaa taaatccatg tttaagtggg tcttcttcat ctattacaaa
     9781 atgattaaat ccagttatat aagcagaacc agttatttta ggtactacag cattgaaatc
     9841 tgcaacttta gtttcttcaa ctatttcacc tttgaataaa gttcctaata tactttcata
     9901 tacgaatttt tctcctactt ttaattcacc tttagcatgt aaagtagcta atttagcact
     9961 tgttccagtt ccacatggag atctgtcaac ttgaccttga ccaaatataa ctacattttt
    10021 gtaagtagct tctggatgag ttggctcatc atatatttca actaagtcta cagtttttat
    10081 atgagctaaa gttggatgtt gtatttctat tttctcattt attatatctc taagtttcat
    10141 agctagttca gttaatttac cagcattttg aggttcgatt tttaaaccta attgacttgc
    10201 atgtattata gcaaagaagc ttccaccaaa tgatatgtca aatttaacag tcccaacacc
    10261 tggtaagtca acttcaacac cttctttata taagaaagct ggtacgttta agaatgatac
    10321 ttctttagct tttccatcta caactgtaac gtctcctctt attattcctg ctggagcttc
    10381 cataactaca tgagttacag gctctactgc tggaactaca cctgtttcta tagctgctgt
    10441 catagctcct attgtaccat ggccacacat gttaaggtat ccaccaccat ccatgaagat
    10501 tattccaaag tcagcatctg ggcaacaagg ttgagtcatt acagaaccaa acatatcatt
    10561 atgtcctctt ggctctaaca ttatagcagt tcttaaataa tctagatttt cctctaaata
    10621 ttcttttttc tcaggcatag aatttccttt tatgttaggt attccaccta caactattct
    10681 agttgcttca cctgctgtat gagagtctat agcttgtata cttctgctaa atttcatatc
    10741 aaattccccc ttaggtttat tttgcgtctt tacgcatttt taattttagg aaagccaatg
    10801 cttttttaac atcaacttca tccatgtgat tgtctccaac tacttctgtt tctatgtagc
    10861 ctgttgtctt tttataatca attatggtag ctttatccaa atatggattg gtaattacta
    10921 atccatctgc tcccataata gtaagaccta ctgcaggtat atctgatttt acaaactctg
    10981 ctatagcatt tgtaaaatca atgtgctggt ttcctgccat atctaaagtt ataatcactc
    11041 catcaggtcg caatgattca atccatcttc caagtctagt tgctaaaaat gttttccatt
    11101 cattgtgctc tgatgtccca actacaacca ctcctaacaa gtctatgtca gaatcattat
    11161 ctatcaaatc tagtaacggg tctctaaata tatgaagtga tgtgtttttc attgatgtag
    11221 aaattcccac aaaactcatt cccttactta aattttttat ccatgttttc tcattttaag
    11281 ttttaaaaat gctaaagctt ttttagcatc tatttcattt gtgttgtttt ctccaactac
    11341 ttctgtctca ataccttcct ttgatttgtt catatcaact atggtatcca tatattgatt
    11401 gcttacaaca aacttagctt gagtcccatt aaatgtaact ccaactacag gtatatctct
    11461 ttttcctatt tcctcaaatg tgttagcgta gtcaacatgt gagtttcccc aaccatctga
    11521 tgaaactata gctccatcta ctctcatggc ttccagccaa gcggccgttc tttgtcccac
    11581 aaaggtttta ttttcatttc catctggagt tcctactact attactccaa gtaaatctac
    11641 atccttatca ctttcaacta tgtctaaaag tgggtctcta aagtgatgaa gagatgtttc
    11701 ttttgttgaa ggtcctattc ccataatatc cacctctctt aagtcatagc tctaagggca
    11761 ccatctctgt attcattagg agatataatc ataggtacat ttcccatatc tataattgat
    11821 gttcctcctt caaatccact aggttccttt gagaataact gattgtcata catagcacct
    11881 tgacctgcta tttgttttac tatgacaacc ttttttgcat ttggtcgaat tttgtcaaaa
    11941 tattcacgaa cttgagtagc atttcttccg tcttccattt tcaaagacgc tcttatttct
    12001 tgtataaaat catcacatgc tctgaatgct gcaagtggaa gtgttctttc aaaaggtaaa
    12061 cctcccttta aagttacatc tacatggatt atataatctt caactgatgg agttccatat
    12121 ctcccaaata ccatttggtc tttcaaaatt ccttctgaag aaccaaattc atgcatttga
    12181 tttccatctt catctgcacc agttaacatt acatatacac ccgttaaagt atgtgtgata
    12241 ccctctccca gtctgcctaa aacttttgtt gatattggta taatgtccat aatagtatta
    12301 atttctctat cataatctcc aggttttata atgtctactt ttatgtctgt tattaactct
    12361 tctctttctt ttatttcatc tatacttgat aaatctattg taagtacatc tttctttata
    12421 gaagtttttg aaccgaaatt aacttcattc atatgaaacg gttttataac aagtcttctc
    12481 attatttttt cttccataat atccaccttt gttcttaaaa cccctctcct aaccgtgtag
    12541 gagaagggtt ctatattttc aaatataaat tacattttaa acgtgagctt tatattcgta
    12601 tggtaatgga actatcttac cagcagatgg tattgcaact aattgctcta atgtatctct
    12661 taatatatgt gtttgcattt ctatattatg tggttcacca gcattagcac ccattggaac
    12721 taatggagca actgctctag gagttccgtt ttgtctaact actggaggaa gagctgctat
    12781 tattattgta ggtattccag cttcctctat tgctctctgc acgatcacgg cagttctatg
    12841 gcaagtacct cagccagctg tcataactac accgtctact ccctcttcct taagtttagc
    12901 agcgatagca ggaccagttt cttcagtgaa gacatgttgg tttcctccac cacccatgaa
    12961 tgcatagtgc ataggagcta ctcctttgat aaatccttct gcagctaatt catgtaatct
    13021 atctataggg aacatacagt ttatatcttt gtttacatct ccattgtcat atccaccatg
    13081 tgataccatc atttcatcaa ctgtagctgt attaggtata gctctaaatg tagtatctcc
    13141 tgctagatta aatctcttgt cagattttaa atgaacacca gctgctgtag ctaaagctat
    13201 agtcatatct tttaattctt tagttacagg agtccaaact ggaggaggtg ttattggaac
    13261 gaatatttca gattgaagtc cttgtactgt tgtaaggctc atatatttcc tctcccttct
    13321 attttcaaac gattatttat tatttttctt cttgttttga ttgcatttct gcttgttttt
    13381 ctttttctct cctaatattt tctacatact cttgattagg ctctggactt aatacctcag
    13441 gatagcttag catgaatcca acttcttgac ctatcttaag ttcgtagtca gttataagca
    13501 ttctattagg tattttaaat tgtccaagtc taccttttag gtctatagtc actccaccac
    13561 ctttgatgtc aactataata ccttcataaa aagtctctga tgagatatat tttaacctat
    13621 ccattataat tctacctctc tatactgttt taaacttgga tctaaattta tgttattgtc
    13681 taaatcttag ttagtatcta aattttagtt ttcgtctttt tcgtatattt ctcttctctt
    13741 cttactcatt ggtagagatt gctcattctc ttctaatact attttttctc ctgtaacttt
    13801 ttctatagct tctatgttat ttaatttaac atttggattc cattttcttt caggagcttt
    13861 aacttctaca ccttctatag catttttaag catagccatt attctaacag catcttctgg
    13921 agctaaagtg ttgttagata atatttcatt ctctatacct tgcttagact tattgttgtc
    13981 taccatgtga gtcatgtatt tattaccaac aactagagca ccttgaacag ctgagaaact
    14041 tacaccaact actggtatac ctctcatacc tatttgctct atgtgagatg cgaaatctat
    14101 atggttgttt ccgaaacctt cagtagttac tacagctcca tcaacttcca tagcttcaac
    14161 taacatacct aatctcttag atacatagaa tttctcagca tttacttgtg gagaaccaac
    14221 aaatacaaca cctactaaat ctatttcttc gtcttccata gctcttatta ctaatggctc
    14281 tctccagtaa tgtcttgaca tttcttttga tgcaggtcct atacaagtta atgcatggat
    14341 accaccatct aatacttcaa gtggagataa tacaactggc atgtttccta agtcaacgtt
    14401 tggttgagct cctaatgtac caactggctc aactggtaat attaggttat catgcattgc
    14461 accttgtccc attatttctt ttataacaac aactttcttt ttaccttctc ttctgtattg
    14521 ctctattact tcttcatcaa ctactaaaga gttgtcaact ttctttaatg cttctcttat
    14581 ttcttgagtt acatagtcaa atgcacggtg agcagctaaa ggtcctggtc tttccatgtt
    14641 agttcctgct tttattgtta cttgaccttt gatgaatatt tcacctttgt cagcagcacc
    14701 tggtctaccc cacattatag tagtatttaa ctcaccttct gaagaaccaa attcacctat
    14761 ttgaactcca tcttcatctg taccagttaa taccattaca gctccatcta taactctagt
    14821 tatacctgaa cctaattcgc cttcttcttt agtagcgatt ggttgtatat ctaatacagc
    14881 ttcactgtaa gtttcatatt tatcaggagt tattatttct aacttcatat ctacaactaa
    14941 ctcttgagtt tcattagctt ctttacatat ttcttctgcg tttctgatgt ataaagtagt
    15001 tccttctatt ttagtttcat cagcaaattc aactttatct attttaaagt gttttttagt
    15061 taagcttctt aaaagtttct tttcttcaac ttttggagct acttctgctg ctgcacttgc
    15121 cactggagca gcaacgtttg ctactggagc agcaactcct gcttgaccag ctactgataa
    15181 aggtatctct aagcttatgt tcttaccttc acctatgtgt attttaactg tttcacctgc
    15241 aactcctgtg cttactggaa ctactgctgc ttctgatact ggagctgctt cttctactgt
    15301 ttcttctact acttcttctt tagcctctcc tgctttgtat ccttctacgt tatctgtagt
    15361 cattggagaa agtgcatcta aagtttcttt taacttagct cctagtactt gacctatagt
    15421 taatccattt tctggtattg ttaataatcc agaatcctct aagtctgcaa atattgctgg
    15481 atcttctaag ttttctggcg ctataattgt tcccgcttca aatctacaac aacaaactgc
    15541 tgggtcattt gcatgggctt gagctgtttc taaagttatt gacatagtat aaaatacctc
    15601 cttacattat tttcttattt attattttaa atctcaattc ctcaacaggt aaccccttaa
    15661 ctgagaaatt aagtaaatta aaacctacat aatttgttat gattaatatt atttttttct
    15721 cactcaaagt cactttatat tataagcaat tcctatgcca acttattaac aaaaagattt
    15781 tttctataat agattcttca tctatcgcta tttttagcca aatattatat aaatatttat
    15841 ttaaaatatg ttattttttg tatttgtaaa cattttctca aacaaaaaaa gtgtcaatta
    15901 atcaacactt tttcattttt tttaacacat ctatatacac aacacttata caagtgttgg
    15961 ctttactagg ttttaaatta aagtttatca ttttgtcaaa atttagtcat atgtcatatt
    16021 ttagacacat ctattttgta ttggtctatc ttataataca aagttgttct gggtatattt
    16081 aatatcttag ctgctttagc tttatttcca ttagacattt taagtgcctt ggttatattc
    16141 tttatttcaa ttttttgagt tgccttcgtt aaatctaagg gatactcttc ttcttcagta
    16201 ctattatttg tagaatccat catataactt ggtacatcat ctacatctat tttagaattt
    16261 tgtgacaata caacaatatt ttcaatcgta ttttttaatt ctctaatatt acctttccat
    16321 ctataattct gaaatatatc tattacatct ttgctaattg taagaacagg cttattattt
    16381 tgtttacata tctcctctaa aaagctatga accaataatc ctatgtcttc ttttctctct
    16441 ctaagtggag gaatctttat ttcaacaaca tttaatctat agtataaatc ttctctaaat
    16501 ttttctgcct ttaccatttt actcaaatct ttattggtag ctgatattat tctaggattt
    16561 atttttattg tggtatctcc accaactctt cttatttctt tttcctgtaa tactctaagc
    16621 aactttgctt gcatactaag aggtaaatcg gcaatttcat ctaaaaatac agttccatct
    16681 tttgcaagtt caaatatacc tattcgaccc tttttacttg ctcctgtaaa tgcacctgat
    16741 tcatacccaa aaaactcact ttcaaataac tcatttggta tggcactaca gttaacaggt
    16801 ataaattgac cttttctctc actataatca tgaatagctc ttgcaaatac ttctttaccc
    16861 gtaccacttt caccccaaat aaatatgcta ctatttgttc tagccacttg ttttgcaatc
    16921 gctttcgatt tttccaattt ataactttta cctaaaatct tatcaaaact acctttagat
    16981 aaatttttga cttcgttctt tagatatttt aatgtatcat ttactttttc aagttcaaga
    17041 gctaatatct tagcttctgt aacatcaact tctgtacata caacaccgat aaattcttct
    17101 ttataaaata taggatttgc atgaactaat gcatacatat tttctttttt atctgtaaaa
    17161 tacaagtcac tcattccaac tttactattt agtacttttt ctgaaatagt attttccaaa
    17221 aattcattca tcggtcgacc aactatatct tcagacttta tatcatacct ttcctccata
    17281 aacttattcc acaagataac aacaccttcg ttatttatag cacaaattcc ttctttaatt
    17341 tgacctataa tatacttcaa agtcaatcca taatcttcta aatgcatata tagatagtcc
    17401 ctaatgtgct cttgttttag aactccaata attttattat ctctaagaac aggaagaatc
    17461 cctatatttt ttaatatcat tatatctcta cattcatcta aggttaaacc ttcgttgaca
    17521 tatattacat ccttcttcat tataagtttt actggttgac cttcatactt tttatgcatc
    17581 tcataaagat tatgtatatc cgtcatggat ataatgcctt ttaattggtc agatgaatct
    17641 attaccatta gagtttttgt gttgcttttt atcatttctt ttattgcatc ttcaattctt
    17701 gtgtcttcgt ctatagttgt aaattttgta tccattacct cttctacttt ttttacttct
    17761 ggtatcgaaa acataaagtc ccccccttta tagtagtacc tcatcaaaac ttatttatat
    17821 atatttattc tagcatatta taataattat ttattaaata attattatta aaatttatta
    17881 agtaattagc atttaaattt atataatcac tttaaaattt ataattaaat ttatacaaat
    17941 gaaatcaaat gaattatacc cttagtatat tattttttca atactatttt caccttcaaa
    18001 acatttttct attcatcaaa caattaatga atgcctgtat ccaatcataa atttaagaat
    18061 ataattaaaa tagatatttt taaacaaact atatttgcat attatattta cataaaaaag
    18121 tcccaaaatg acttataaat caatttgaga ctttttattt taaacttaat aataatataa
    18181 attaatttct ttttataaat taaggcgtaa aaccactata cctttaggtt ggtggatgta
    18241 aacctatccc ttactttgta tgtatttttc aatcatttct ttagaaacat ttcctacact
    18301 gaaagcaaag tatccatctg agcaaaaagt tttttctgta tttacataca aatatcaaat
    18361 gtaccaataa caaacactta gaatggtttc tactaacata attcacaact ccaattcaat
    18421 cccttcttaa atatatatgg tttaattagt tttacatttg gcactttttt gatttctttt
    18481 aaagctatat attgccctca aaatacatta aaaaacatcc ttagaagtaa tttttaagtt
    18541 ttacttctaa agatgtctaa cttctattta tctattattt tatttttaat cttacgtcac
    18601 cttttattct tcttattaat gggtgattaa ctgttctcat tacatggtca gtaatatgat
    18661 atacacctaa ctttaatttt ggtgcagaac ccattactgt gttactacag tcagtacagt
    18721 ttcctattaa tataacttct gctccagctt tcttaaattc aatacatttt tgagcttgtc
    18781 ctggacagtg tcctggattt tcacatttta atgcaccttt aatttcttga gcttgtttac
    18841 atttttgaac tgatactata tcagtaactc ccattttagt tgctatatct ctcattctct
    18901 cttcattagg tccacatgca cagactaaaa gacccatttt tctcttttca ttaacaaatg
    18961 gcatagtaac tattatacca ccactagttt tagttattgg agcatctaaa gtagtagctt
    19021 taccagtgaa aggtcctcct aggattattt caccatattc accttttatt ccaccagctc
    19081 tttcaattaa ttctccgaca gttgttccaa ttggcacatc ttcaaatact atagactctt
    19141 ttccactgtt taattgacca actacagtta tatttttaga tataactggt cttttttgct
    19201 caacagcctc tactattcta gttagtgttt ctacgtttat tactactgca ttagcctctg
    19261 aaggtaattg agttggctca agtaattttc ctagaacgtc tcttactatt gctctctctt
    19321 ctcccattgg gtacatatca actagttctg caacttctat gttgtctcct ggctttatta
    19381 cttttttgaa agcttctatt gcttttggat gcttgctttt tattgctagc ataccttttc
    19441 cagcattagt aacttccata gcatatttta taccttgata aactatttct ggtctttctt
    19501 ctatttcttt tatattatga gctaataaag gttcacattc agctgcattt gctaatattg
    19561 ttccaccatt taaatctatg tttaatttta catgagtagg gaatcctgct ccacccattc
    19621 ctactactcc agcttcttta attaagtcaa gtataccttc tccttttaat ggttcaaaag
    19681 tatcttcttg tacttcatca gcttcaatta ctatcgcttg gtcatttatt tcacttattt
    19741 ttccactaac actggcataa atatttgccc ctaatccagt tggttctgcg attagagttc
    19801 ctttttttac tacatctcca acacttacta ctggcttatc tggaccacca acgtgttgtt
    19861 ttaataaaat agaatatagt ttgttcaact agtacgcccc cttaagtata ttaatttttt
    19921 tataagctgt tcaaataatg ttaatgctat ttttctatta taaggtctta acatattgtt
    19981 tatttctata gaacatatcg cttattaaca aagcaatttc tatgccaact tttaaacaaa
    20041 cttataccct ttggaaatac tgtcttacag cattcattaa ttcttctaaa tttttaaatc
    20101 catcaaaaaa aagtcaatta ttagacactt ttttaatcaa tttaatctat aattattgat
    20161 attgcataca tgtataaaaa gtgtcaaaaa atagacaccg tctttttatc gacacttttc
    20221 taacatatac tatataattt atcacaactt tacttaaaat atacatttat tacttattaa
    20281 ttcttagttt tttgcactcc attggaatca actacataat tatctttata caacaaatca
    20341 gatattttga ctagactgta tccatctttt tttagtcgac ttattatagt atccaaatag
    20401 ttatctacat catttatatt agcatggaat aaaactattg aaccaggttg agattctttt
    20461 attactttct caattacatg atttggtcca agctccttcc aatctataga atctacatcc
    20521 cacttaatta ctttataacc taaatcacta caaatgtcca tagctttatt gtttacgtcc
    20581 ccaaaaggag gtctaaataa attagtcttt tttcctgtta tattatatat tttttctgat
    20641 gtagtttcta tctcttttgt tatgtcttct tttgatattt ccttcaaatt ggcatgagtg
    20701 tttgaatgat ttccaatttc atgtcctttt tcatctatag cttttacaat ttcttcatta
    20761 tcatccaccc aactaccaac taaaaagaag gtagctttta cattatgttt atctaaaatt
    20821 tttaaaatat catccatatt atcagttccc catgctacat caaaactcaa ggctattttt
    20881 ttctcatttg tatcaacttt atatataggc atttttgaat tgtcgtattt agaacatatg
    20941 tattttattc cataaacaaa aacaaatatt aacactaata ataatgaaaa aaataaaaca
    21001 ctatatatca aagctttagt ccttttaact tctcttttca tactattccc cctttgaaat
    21061 aatttcttct atatttaaaa tttatttttt ttcagctcaa aaaagtactg taatatttaa
    21121 tttataagac aataactaaa aaaataaaac ctatattgac ctaattaaac tctctaaaat
    21181 actcatttat ttaatttttt tatatttttt acatttttac ctttattttt ctgtatatat
    21241 tatttttatt ttggagaaat tagtattaac atcacataaa taaaagtgtt gttagacatc
    21301 ataaactaat tatttgagga ggaaatttac tatgtcaaat tcaaacagaa cagtagttcc
    21361 agaagcaaaa gctgctttaa accaaatgaa attagaaata gctaacgaaa taggtttatc
    21421 taactatgaa aacatagata aaggtaactt aacagctaga gaaaatggat atgttggtgg
    21481 atatatgact aagaaattag ttgaaatggc tgaaagacaa atggctggaa aataaatagt
    21541 aaattttgac acctgggtat caacagggct gctaattagc agccctgttt ctctatattt
    21601 atttataaaa ttgttttata tgaattatta aagattaata atacaataca aattgttctt
    21661 ttaattttgt acaaattagt ttaatatatt atatatagaa atagtttgac ggaggatgat
    21721 atttatgttt gaaaactcgt ctgaagaatt agcctatcac aaacttttaa ttttatatat
    21781 attagaaaaa attaaaatgg atttaactaa ttctcaaata actcaagtag tattagagac
    21841 tgagatgatg aattattttt cattacaaca gcttttatct cagcttatgg aatctaagtt
    21901 tttgactaca tataaagact ctgacagaga atattatgct cttactcaaa gaggcgtaga
    21961 aagtctagaa tacttcttta atcgaatacc tactagtgtt actgaaaaaa tagacaagta
    22021 tatagattct aacaaagaaa atctactagc agatactcaa gtaaagtcta gttttgtaaa
    22081 acaaagtgat aatgaattta ttgtcaacct tagagtcata gaaaatcagt ctaacttaat
    22141 agatttaaat ctaaatgttt catcagagaa acaagcaaaa cttatttgca ataactggaa
    22201 aaataatgca tcttatatgt atgccgaagt tatcgattta cttataagag acatacatta
    22261 aagcctacaa acaaattata gttgtaggct cttttcctac cttgatttct tttattaatt
    22321 tattgtttgc agtttcataa acttttactg aattttcttg tgtatctgat acaaatagta
    22381 gcttttcatc tttagatatt tctaagcctt gaggaatccc atttatttca atatttccaa
    22441 tgtatctact cgttgtataa tctaaaattc ttacattatt ataacccaaa tcagcaacat
    22501 aaactttttt gccactataa ttaaatctta tttctactgg taataaaaat tcatataata
    22561 ccttttttat atccatgctt acttcatcta ttattataag ctttccatca agtgttgata
    22621 tatacatttc tttcttttgt ttatcaagct ctatatgcca agctctaaag tccatattta
    22681 ttttcttttg tatattttta ttaattgtat ctatacatac tatcgagttt atacacggta
    22741 catataattt tttactttct ctatcaaagt caaatccatg tggcatatta tcaactccaa
    22801 taattcctat tggatttaat gtgtttttat ctagtacata tatactatta gaatcttcat
    22861 ttgccacaaa caattcatta tcactaagta caacttgact aagacttcct cctatagaga
    22921 cagtatcaat tattttatca ttacttaaat ctaagacata aagtatccca ttacttgaac
    22981 taggtatgta tattaaattt ttttcttttt ctatgcaaaa atggtgtgga tatatattat
    23041 cttctaatac tatttctttt tctagttcaa gtgtagaata atcaactatg ctaatactct
    23101 ttgataagta gttagaaata tagattttca tataacccct cctctctaca atacatatac
    23161 tacaataata tattatttca ttagcttgta ttatgcttta tagttgcatt attacgcata
    23221 tatttggtat tattattatt gttaaaaaaa gaaataatat ttaatggagg tctttttaat
    23281 gaaagtaaca tttaatccaa gtggtgtatg ttgtagagaa atgatttttg aagttgatga
    23341 aaataatgtg attgttgatg ctgagtttat aggaggatgt aatggtaatt tatcaggact
    23401 tagaagctta ataatcggcc aaaatgctct tgaagttgct gataaattaa acggtattga
    23461 ctgtggtgga aaaggtactt cttgcccaga ccaattatct aaggctatac gtcaatctat
    23521 ataattactt atataaacat cagtaaaatt attaaaaact tactttatat aaaaagagtt
    23581 gtccattttt tagacaactc ttttttatat ctttatttgt atatttattt tataaaatta
    23641 taatttcttt actaaagttc tcactgtacc aatcatatat aatgaacctg cacttattat
    23701 aatatcatcc tcacttgatg tttctaatgt ataatttact gcatcctcta tctcatgctt
    23761 tgatgttaca tcatctacat attttaatac tttttctctt aatatatctg aatttatggc
    23821 ccttgggtta tttggtgttg ttgttattac cttattaaaa tgtggcaaca atatctctaa
    23881 aacactatca atatctttat cttctaacat acctattaat aaggttaatt ttctaccttt
    23941 aaaattctta tcaagtgctt ttgccaaaga ttttgctcca tcttcattat gtgcaccatc
    24001 tattataaaa attggatttt cttttatctt ttctattctt cctggccatt tagtatttat
    24061 aagtcctttt cttatagatt cttcatttat atttgctaat ttatttatgt cttttagata
    24121 ttttattaca ctaattgcca agatagaatt gtttatttga tgttcaccta tcaattttat
    24181 ttcaaggtct ctataagttt ctttcattac agtacaatcg tatatttgag aatttatatc
    24241 tgattttttt atgtttatat catcaaaatc aacttctata tatttagctt ttttctcttt
    24301 acaaactgac tttataacat ccttcgcttc atcagtctgg tcatatacta gtactactcc
    24361 attttccttt attattccag ccttttcgta cgctattttt tctattgtat ctccaagtat
    24421 acctgtatgg tccaaactta tagatactat cacacttaca agtgattttg ttattatatt
    24481 tgtagcatca tatctgccac ctaaaccaac ttctaatgcc acaaagtcta ccttttgttc
    24541 tgaatagtaa taaaatgcca ttgcagtaac cacttcaaat tctgttggat atgcatagcc
    24601 ttcttttacc ataatctcta ttttttcttt tattaactca attattctag caacatcatc
    24661 ctgtggtata ttttgtccat ttactcttat acgctcagta aaagtttcta aatatggaga
    24721 tgtatataaa ccaactttat atccacactc tcttagtata ttagaaacaa aagaacacac
    24781 agaacctttt ccattagttc cagctacatg tattatattc aaagtttctt gtggatttcc
    24841 taataattct aataactttc cgatattttc taatcctaat cttataccga atttattagt
    24901 ttgtgatatg tactctaatg cttcttcata tttcattatc taactccccc tgtattggca
    24961 ttttctctta tgctatctaa ttatagtata ttttttactt ttaatctacc gacaaagaat
    25021 acatataatt taataccttc tacattaaat ttagctacaa atctttattt ataagttttt
    25081 atatctttcc actaattcag tcagtgtata tattgaaact aagtcatatt ttgagatgtc
    25141 catataatta aactttatgt caaatctatg ctctagcttt ttcaacagat taaaacaatt
    25201 atcataaacc aaacctatct ccttattaaa aaggctaccc ttaaagagtt tttcattatt
    25261 atcttcaata tctaaacaat ccctaacaac gtcagcaact ttcataagaa gagcattttc
    25321 tttgtcatac aaatccaatt taaagtttac aattctacta cactcatcaa aatcacaact
    25381 atcccaatga tttctagttg ctaatgattc aagcttatca tttaaatcag atattgttat
    25441 gcctggtttt tctgataata tcttagctat tttaccagta agctttgctg ataaataact
    25501 attacatttt ggtggtaatc tataggaatt atttatatca caacttatat atgaattgct
    25561 atccataaca cattgaacat catatttttt gttgtaccaa aatgaatcat ctccatctaa
    25621 agtaaatcct cttacaccta taacattgtt aaacatagct ggataactta aatcaaaatc
    25681 attagcgagt gaacatacta ttatttttcc ttttctaaac aattcgtaac aaacttcaaa
    25741 taagccctta acactctcta ctcctatgac tgataaactt aaattaatta atcttatgtt
    25801 agtgtctaaa aggtatctta atgcttcttc aagaactttt atatttgtaa taccatcttt
    25861 tctcaaaatt tttattacaa ataactctac atcttcatac tctttcttta ttattgatgc
    25921 acaagcagtt ccatgaccat cttcatcatc aaatttatct gatataaaga tataatcatg
    25981 catacattca aaagcaattc ctccaactaa attatctttt aagtaatcat gttctttgtc
    26041 tattcctgta tctaatactg caaccttaac tttattattc atatcacata tattctccat
    26101 tacaaaagtt tttaatatat ctatgttaat agttcatctt aaactttgat ttaagttgca
    26161 caatttatct tttaaatgct atatttcttg ctaaaaacat aaaataagta caccatatat
    26221 ttaatttata tttattatat caattattat cattaaatgg ttaaatgtta agataaaatt
    26281 tttagattta tatcacaatt taggattatt tgtaaaaaag gagattatct ttttattaga
    26341 caatcccctt gtatatttta ttaatattta tctatgatta agcatcaatt ttattttata
    26401 ctttttgatt ttaatactat acatctaaat cttatttagg gggaacaatt atttatatga
    26461 tataaaaatg ctaatttgaa tgaacttgtc ttataatcag ttttataaaa tacaccattc
    26521 tacaaagtat gaatcaaaag gtgaaaaaat actaaaaata ataaatcaac atcaatttta
    26581 accaatatat gaaaaactta taccaagtat gcataaaaac taatataaat attat
//
LOCUS       DAELXX010000053        26194 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.053, whole genome shotgun sequence.
ACCESSION   DAELXX010000053 DAELXX010000000
VERSION     DAELXX010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26194
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.053"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(157..912)
                     /locus_tag="KPH29_002047"
     CDS             complement(157..912)
                     /locus_tag="KPH29_002047"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBG1467639.1"
                     /translation="MLKVIINGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVTEKTDVIIDFSHHETLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPESENTHGRYGRDCKRKPNEIGIHSIRGGNIISDHDVIFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
     gene            1353..2342
                     /locus_tag="KPH29_002048"
     CDS             1353..2342
                     /locus_tag="KPH29_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBG1467640.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMIVLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVINATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
     gene            complement(2613..3329)
                     /gene="dapD"
                     /locus_tag="KPH29_002049"
     CDS             complement(2613..3329)
                     /gene="dapD"
                     /locus_tag="KPH29_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBG1467641.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     SNGSYIFVGDYEDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLNEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(3553..4302)
                     /locus_tag="KPH29_002050"
     CDS             complement(3553..4302)
                     /locus_tag="KPH29_002050"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBG1467642.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDNEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            complement(4369..5256)
                     /locus_tag="KPH29_002051"
     CDS             complement(4369..5256)
                     /locus_tag="KPH29_002051"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBG1467643.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            complement(5373..6374)
                     /locus_tag="KPH29_002052"
     CDS             complement(5373..6374)
                     /locus_tag="KPH29_002052"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBG1467644.1"
                     /translation="MKKVNVAIVGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     gene            complement(6799..7680)
                     /locus_tag="KPH29_002053"
     CDS             complement(6799..7680)
                     /locus_tag="KPH29_002053"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBG1467645.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGVISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
     gene            8003..9196
                     /locus_tag="KPH29_002054"
     CDS             8003..9196
                     /locus_tag="KPH29_002054"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBG1467646.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            9402..10520
                     /locus_tag="KPH29_002055"
     CDS             9402..10520
                     /locus_tag="KPH29_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HBG1467647.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKEAID"
     gene            10651..10896
                     /locus_tag="KPH29_002056"
     CDS             10651..10896
                     /locus_tag="KPH29_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HBG1467648.1"
                     /translation="MKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            10883..11629
                     /locus_tag="KPH29_002057"
     CDS             10883..11629
                     /locus_tag="KPH29_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG1467649.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKNYDIAGIDISDEMLSVAREKAEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENVFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            11914..12789
                     /gene="hslO"
                     /locus_tag="KPH29_002058"
     CDS             11914..12789
                     /gene="hslO"
                     /locus_tag="KPH29_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBG1467650.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPDDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            13180..13980
                     /locus_tag="KPH29_002059"
     CDS             13180..13980
                     /locus_tag="KPH29_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBG1467651.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGTHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            14507..16747
                     /locus_tag="KPH29_002060"
     CDS             14507..16747
                     /locus_tag="KPH29_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG1467652.1"
                     /translation="MSNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNDIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSRDGEISEATKKKLSKLLIEDRCPICDGRRLNEGVYRSLI
                     NGYNIADLTSMQIDELAEVIKSINEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHVVDVGPKAGKYGGKVVYEGSYKNLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEIKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNVCEGKKFKKEVLEYRFQRKNIIEVLEMSVLDAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            17161..18696
                     /locus_tag="KPH29_002061"
     CDS             17161..18696
                     /locus_tag="KPH29_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG1467653.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTAMTISGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIATIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGIGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNISDLKNVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVIVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSEVFKMVGDILTDKEMAKMNYDVENNNLEPREVACEFLNKK
                     GLL"
     gene            18712..19473
                     /locus_tag="KPH29_002062"
     CDS             18712..19473
                     /locus_tag="KPH29_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1467654.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            19811..20679
                     /locus_tag="KPH29_002063"
                     /pseudo
     CDS             19811..20679
                     /locus_tag="KPH29_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            20873..21124
                     /locus_tag="KPH29_002064"
     CDS             20873..21124
                     /locus_tag="KPH29_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBG1467655.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
     gene            21236..22015
                     /locus_tag="KPH29_002065"
     CDS             21236..22015
                     /locus_tag="KPH29_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG1467656.1"
                     /translation="MNNTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFIDYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLELENDVEEEMNWIITRAN
                     ELIKKYPSQKDMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(22150..22800)
                     /locus_tag="KPH29_002066"
     CDS             complement(22150..22800)
                     /locus_tag="KPH29_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467657.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDSTSK
                     YPDALNAILNCDRLIIAFPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIDHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(22797..23300)
                     /locus_tag="KPH29_002067"
     CDS             complement(22797..23300)
                     /locus_tag="KPH29_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG1467658.1"
                     /translation="MKLLIHDLTDKELEKLLPKLDDNIKILPNTKEIHKCIGRFGCWI
                     KTPSECVVKDYYSNMGKLINESDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            complement(23349..23864)
                     /locus_tag="KPH29_002068"
     CDS             complement(23349..23864)
                     /locus_tag="KPH29_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG1467659.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGSKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKIDVEENHCIDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            24180..24671
                     /locus_tag="KPH29_002069"
     CDS             24180..24671
                     /locus_tag="KPH29_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG1467660.1"
                     /translation="MRTIDIKGENMRELLKWVSIINRYNTNYIDRKLADIGINNSQYF
                     YVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGVKDKRTWHLYPTE
                     KSLECYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDMNEEAKLEAKENG
                     RKI"
     gene            24655..26058
                     /locus_tag="KPH29_002070"
     CDS             24655..26058
                     /locus_tag="KPH29_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG1467661.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQFFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
BASE COUNT         9704 a         3227 c         4334 g         8929 t
ORIGIN      
        1 tataccaata aataagataa tctttaagaa aaatgattat atttaaaagg taagcataaa
       61 atttataaac ttttagaata gaaatctcaa tcaattagat attcaaattg atgaatatga
      121 atacctaaat agattgagat ttattatgta caaaacttat cctagtatat ctgacatttt
      181 ataaagtcca gattgcttac caattaaaaa tttagctgca actatagaac cgtttgcaaa
      241 tacttcatca gattgagaat gatggcttaa tgtaagaact tcattatcac ctgcaaatat
      301 gacatcatgg tcgctgatta tattgccacc tcttatagag tgtattccaa tttcatttgg
      361 ttttctttta caatctctac catatctacc atgtgtattt tcactttcag gcaagacttc
      421 tctaattgca ttagctatca tagtagcagt tccactaggg gcgtcttctt ttctattgtg
      481 atgtttttct atgatttcaa tatcaaaacc ttctaacatc tttgaagcat ccttaactaa
      541 ttttattaat atattcactc caagagatgt atttgatgat aataatatag gaatgttttt
      601 tgatgcttct tctattttat ttaagtcatt ttcatcatat ccagtagtag ctaatactac
      661 aggtgtatta ttttttagag catatgacaa tattggctct aaagtctcat gatgtgaaaa
      721 gtctattatg acatcagttt tttcagttac atcagtcatt gttttgtaaa gctttgtatc
      781 actatgatga cctttttcat cagcagacct agctacacca caaaccaact ctaattcatt
      841 atcttttttt acacattctg ctaaaactct tcccattgtt ccattatagc cattaattat
      901 aactttcaat ataaattcct cctatgaaat taataaaaat ttgttatatt tctataagtt
      961 tattttatca ctaatattat ttatataaaa taatattagt gataataagc ttacaattta
     1021 tcttacatta aaaaaattta agtggatttt tcaaaatata gataaacttc tttttcattg
     1081 tataaatgat aaaaatatat ttgacttcat atcaaaaaga gtgccccatg aactaaaaag
     1141 ttaattttga gataccatat tcatatatca tatgatttat ttggaaatca atattaatac
     1201 attaaaataa ctaaatacat gtatgcataa agatgtataa aaaatatata aacatatagt
     1261 attaatttta aattcaacaa tttaattgtt aaaagtgtta ttattaaatt agaaatatct
     1321 taacttaata tactgagatg ggagtgatag atatgttaaa agaagaaaaa acttatcaag
     1381 agaaagacgc aaaagtattc aatggagtgg cagctttgat aatgattgta ttatttttag
     1441 tagtttcaat aggtatcatg gtttttggga tagtaaaact tgatagtgga tttactggaa
     1501 taggttcact tatgttaatt ttgggacttg tatttatagt agtagatttt atcctatttt
     1561 ttgggctaaa aatgattaat cctaaagaag ctatagttct agttttgttt ggtaactatt
     1621 atggaactat aaaaaaagaa gggtactatt gggtgaatcc attttgttca gcaataaacc
     1681 cagcagtatc aagaattaat gtatcgaaat cttcatctga tgaaaaggta gatatatcaa
     1741 ctaattcaag gggcaaaaaa gtatctttaa aaacaatgac tcttaataat gaaaagcaga
     1801 aagtcaatga tttattggga aatccaatta taataggagt ggttgtaatt tggaaggtta
     1861 taaatgcaac aaaggcagta tttaatgtgg ataattataa tacattctta tctattcagt
     1921 gtgattctac tattcgtaat gtttcaagac tttatccata tgatgtatct gaagatggag
     1981 atgaaaaatc tcttcgtggt agtagtcagg aaatagcaga tagattaaaa gatgaattac
     2041 aatcaagggt tgacattgca ggaattgaag tgtgtgaagt gagaattact catctttctt
     2101 atgcaccaga gattgcagca gcaatgttac aacgtcaaca agcagaggct ataatagcag
     2161 cacgtaaaaa gattgttgag ggggctgtaa gtatggtaga gatggcattg actaatttaa
     2221 gtgaaaataa tgttgtgact ttggacgaag aacgtaaagc agctatggta agtaatctgt
     2281 tagttgtgct ttgtggcaat aaagatgcac aaccagtagt aaacagcagt tctatttatt
     2341 aaattataaa aagtttaata gtttattaca ttgatatatt gaaaaattga aggaaataaa
     2401 aatacttgtc atcaagaaat aaaaatgata taagaatgaa taagagaatt gtaaaaaaat
     2461 aaaatgatta taaataaaag ataaaaatat tataaaaaag agggtgcctc tgatgaatta
     2521 aattcataca gagacacctt tttaattaat tttttaaatt agtatattat tgtttaatta
     2581 atctttctaa ttaataatat agtactattt aattagtcta aatttctaag gtcttccata
     2641 agcttagttt tgtcagccgt tttttcatct ttcattttta taactttagc aggagaacca
     2701 gcaacaacgg caccagcttc tacatcagta gttacaacag caccagctgc aacaacagca
     2761 cctttaccga ttctaacacc ttcaagaata actgcattag ctcctattaa aacatcatct
     2821 tcaactataa caggagtagc agaaggtggc tctaaaacac cagcaacaac agcaccagca
     2881 cctaagtgaa catttttacc aagagttcct ctagctccaa taacagcatt catatctacc
     2941 atagaacctt caccaataac agctcctata tttataacag ctcccatcat tacaacagca
     3001 tttttttcta tagtaaccat gtctcttatt attgcacctg gctcaattct tgcatgttca
     3061 tttaaagtat tttttagagg aatagcagaa tttcttctat catattctac atgagtatct
     3121 gttatatcat tcccatgttt atctaatact tccattatat cctcatagtc acctacaaat
     3181 atataagaac cattacttcc aaatacctta aatttatcag ttgattggaa gtgtaaagag
     3241 ttagtattta catatacctt aacaggtgtt ttctttttaa cttcttttat ataccttgct
     3301 atttcataag catcgtttaa atttaccatt tgttaattct ccttgtattt cattatattt
     3361 cttttattat atacttattt gtcaatttta gtatatactt acttcttatt atttatattt
     3421 tattttagtt agtttttatt gtatagacat agtattaaat aaatttatta tatatgtttg
     3481 atttgtattg cgtattatta tatctatatt gcatatgttt aatttatatt gtgtattatt
     3541 atatttatac tattatccca acatgctgtc catattataa aatcctggtt tttgcttaac
     3601 taagtatttg gcagcagcta tagaaccact tgcaaagata ccacgagatt gagcttgatg
     3661 gcttattgta agaacctcat tgtctccagc aaatagagca tcatgttcgc caacaatatt
     3721 tccacctctt atagcatgaa taccaacttc atttggttgt ctttttgcac tacgaccata
     3781 tctaccataa ttattttcaa cgtttggcaa aacctctttt atagcgttag caatcataac
     3841 agctgtgcca cttggggcat ctacttttct gttgtgatgt ttttctatta tttctatatc
     3901 aaatccttct agtaatttag ctgcatcttt aaccaatttt actaatacat ttacaccaag
     3961 agacatatta tatgattgga atacaggaat aactttagcc gcttcacgta ttttgttcat
     4021 ttcgttatca ttatatccag tagtagcaat tactaaaggt gttttagtct ttaaaacgta
     4081 agataagata ttatctaaat tagagtgatg agaaaaatct ataactacat cagcttcctc
     4141 aactatagta ctcatatctt cataaatttt aaggtcgcct tcatagctat ccagacctct
     4201 agctgcaaca caaaccaatt ctaattcatt atcttctctt atagtatcgg ctaaaatctt
     4261 acccattttt ccatcataac cagttgctat aacttttaac ataatttcct cctatgatta
     4321 agggctaact tttgaccaaa cttacttttt accatgcatt aatctttact atgcattaac
     4381 ttttagtcca aagttaacta attctttctt taacacttct aaatttgctg ggtccatttc
     4441 tgctagaggt aatcttaaat ctccaacatt gaatcccaat aaattcattg cagtcttaac
     4501 tggtatagga tttacttcta taaataaagc tgcgattaga gcatccatac caagttgtaa
     4561 ttttctagaa ccttcaatat cgccttcaaa gaatttagct accaaatcgt gagtttcttg
     4621 agggcaaaca ttagcaagta ctgatataac tccttgacct cctaaagata ataaaggaag
     4681 tatagtatca tcattaccag agtagatagc aaagtcatct ggaactaaac tagctatttc
     4741 tgcaacttgt cctaggtctc cactagcttc ttttacagca actatgttat caattttagc
     4801 aagttcaact ataaggctag gacttatatt tacacaagtt cttccaggga cattgtaaag
     4861 tataattgga agtttaacac tgttagctat tgtttcaaaa tgacgcttaa gtccagcttt
     4921 attagcttta ttgtaataag gtgttattat taatagagca tctactccta atttttcagc
     4981 ttcttgactt aagttaactg agtgcatagt atcattagaa ccacttccag ctataacagg
     5041 aactctttta ttaacttttt caacaacata cttaatagta gcaagttgtt cttcatcagt
     5101 catagtatta gcttcaccag tagtaccaca agatactata gcatcagtac cattagctat
     5161 ttggtattca actaattcac ctaatttgtc aaaatcaact ttgttatcct ttgtaaatgg
     5221 tgtaactaga gcaacagcag aacctttaaa tatcatataa aatcaatccc ctctttttta
     5281 tttttgtgtt ttatattaca gttacttcta atgcttgttt ttgagttact attaatattt
     5341 tacaaaatta tatatttata caagtttgta tattatacta aatcgtatga taaagccatt
     5401 tcagctattt gaattgcgtt agttgcagca ccttttctta tattgtcagc aacaacccaa
     5461 agatttacac cattatcaac actaaaatct cttcttattc ttccaacaaa tacttcatct
     5521 ctatcatcaa attctacagc agttggatat tcgtttttag caggattatc aactactaca
     5581 acacctggag cttcttcaag tgtttttact aaatcatcta actcaaatgg attgttaaac
     5641 tcaacattta tagattcact atgaccattt tttactggaa ctcttacagt agttgcagtt
     5701 atttttaaat catagttatc aagtattttc atagtttcat ttaccatttt catttcttct
     5761 ttagtgtatc cattttcagt gaatgaatct atatgaggaa gacagttgta agagatttga
     5821 tgagggtaga atccagtagg attaccgttc attcctttct ctaaatcttc aacaccttta
     5881 acaccagaac cagatacagc ttggtaagta gagtaaacaa ctctcttaat tttgtattta
     5941 tcatcaagag ctttaagggg aaccatagct tgtatagtag agcagttagg attagctatt
     6001 atacccttat gattttttat ggcttctgga ttaacctcag gaactactaa aggcacatct
     6061 ggattcattc tccattgact agagttatct actactaaga ttccatttga agcagctatt
     6121 ggagcatact tttcactaat acttccacca gcagagaata atgctatttg tatgcctcta
     6181 tcagtaaatg aagtttcctt taattcttct actatatatt ctttaccaca aaacattact
     6241 ttagaaccag ctgatttaga agaagagaat aaaaatagat tttctattgg aaaatttctt
     6301 tcttctaata cttttaaaaa tgttctacct accattccag tagcaccaac tattgctaca
     6361 tttacttttt tcatataaat tcaccccaat tttaaattat taaaacatta ttttatttta
     6421 gttatacaag taagatatgt taatattttt atatatccaa aaactttaat attatattaa
     6481 caactttaat tatatacaaa aaacgtacaa aggtatactt tgtacgtcta atttatcact
     6541 caaagtaaac cctgtagctc atcacctaca aaaataagtg acagtattac acatgttatg
     6601 tgcaatccca gaaaatatta ttgcaagaaa tattttcttc ggctgtaaat cctttttcca
     6661 atcattaaca cctgccagct actattggat actcttagtt acagctacct ctaccctagg
     6721 ttaataggat attaaaattt gattattcta ataataatac atatttaaga aaaaatcaac
     6781 ttatttatat aaatattgct aaaacttaaa tccaaaattt gtcaattctt gttttaacac
     6841 attaagatta gtttcttcca tttcagctag aggaagtctt aaatcaccaa cattaaaacc
     6901 taaaatgttc atggcagttt taacaggaac tggatttact tctataaata aagcagcaat
     6961 taaagcatcc atatcaagtt gtaatttttt tgagccttca atatcacctt caaagaattt
     7021 agttactaaa tcatgtgttt ctttaggaca tatgttagca agtacagaga taacaccact
     7081 tccacctaat gataatagtg gtagtatagt atcatcatta ccggagtata tagcaaagtc
     7141 tttaggtact aatttagcta tttctgcaac ttgagctaag tctccacttg cttccttcac
     7201 agcaactata ttatctattt tagcaagttc ggccacaaca gatggcttta tattcacttt
     7261 agttcttcct ggtacgttgt ataaaataat tggcaactta acgctatttg ctattgtttc
     7321 aaagtgaagt ttaagacctt tctcattagt cttattatag taaggagtta ttattaaaag
     7381 accatccacc cctaattttt cagcttcttg actcatatga acagacaaca tagtattgtt
     7441 tgaaccagta cctgctataa caggtattct cttattaact ttttcaactg tatattttat
     7501 tacagcaaaa atttcagatt cactcatagt agtcgcctca cctgttgttc cacaaacaac
     7561 tagtgcatct gtgccattct caatgtgata ttcgattaat tcacctaatt tttcaaagtt
     7621 gacattatta tcctctgtaa atggggtaac taaagcaaca gcagaacctt taaataacat
     7681 ataatcaaat ctcctttttt atctaaattt aattttttaa gtacaataat aattatagta
     7741 caaattaatt aattttaata ggtatagtta atatcttaac tgtttaaata agatatttat
     7801 attatattaa tattagaaaa attttaaata aatgttttat tctcaaaata tgcatattaa
     7861 tttaataaaa tataccaaaa tggaaataaa aaataaattt tatgagaatt taataactaa
     7921 taattaatat atttaggtta taataaatat gtgcgtaaaa tctaacaata agcaataata
     7981 ccgtaaattt aggaggaata atatggatac tttaaaggaa ctttttaaaa taggaagtgg
     8041 accttcaagt tctcatacta tgggaccaca aagagcagca gagagattta agaatgaaaa
     8101 cccagatgca gaaagtttta gagcaatatt atatggtagt ttggcagcta cagggaaagg
     8161 tcacttaact gactatataa ttgaaaaaac tatagcacct aaaaaagtcg aaattgtttg
     8221 ggaagaagat attataaaag attttcaccc aaatgggatg aagtttgaag ctttagataa
     8281 agataaaaat gtaacagctg aatggactgt atattctgta ggtggaggga ctatagcaga
     8341 agaaggtcaa agaaacagta aaagcaatag tatatatcac ttagatacta tggatgagat
     8401 agtaaaatgg tgcaaagaaa ataataagac tttggtagat tttgtgttgg aatgtgaacc
     8461 aaaagacata aaagattata taaaaactat aaaagatgct atgagaaaat ctattgatga
     8521 tggactaagt acagatgaaa taataccagg caagttatta ttaaaaagaa gagcaagtac
     8581 attttataat gcatataaaa aagataagag cttctcaaca cttgtatatg catatgcact
     8641 agcagcttca gagcaaaacg cttctggtaa tataatagtt acagcaccaa cttgtggttc
     8701 tgctggagta ataccaggaa tattctttgc aatgcaggat ttctataatt atgatgacga
     8761 aaaaataatt gaagcactac ttgtagctgg aataataggt aatataataa aaactaatgc
     8821 ttcaatatct ggagcagagg tagggtgtca aggtgaagtt ggagcagctt gttctatggc
     8881 agcagcagca gtagcatact taaaaggtgg aactatagac catattgaat atgcggcaga
     8941 aatagcactt gaacatcatt taggaatgac ttgtgaccct gtatatggat atgttcaaat
     9001 tccatgtata gaaagaaatg caatggcagc tcaaagagct tatgatgcag ctaattatgc
     9061 attattaact gatggctcac attctgtctc cttagaccaa gttgttgaaa caatgaaaga
     9121 aactggtata gatatgatgg ataagtacaa agaaacagct aagggtggac ttgctaaaca
     9181 tttcttctct tgctaaaaat aaaattttcc aataataatt cctctgtttt gatttatgaa
     9241 acaggggaat tattattaat ggtgaattat ttaataatgc aaaatatttt tatattcaat
     9301 ttagcttttt atttttaatt agtataacat gctatataat attttaatta taaagaaata
     9361 tagaaatatt ttttaataaa ttagaaattg gaggagatta catgagagag gcatcatatt
     9421 tacaagaaca actttataaa cataggaagt cattaaatcc tattgcagag attgggagaa
     9481 aagagtataa gacttcaaaa tatataagag attatcttga taaattagga attgagtatg
     9541 aagtttattt agaaacaggt atagtaggtg taatagaagg taaaagacct aaaaaagata
     9601 ttgcttttag agctgacata gatggtcttc atagtgaaga aggggtaaaa catttatgtg
     9661 gtcatgatgg acatgctact atattacttg gattaattga gtatttaaat gataataaag
     9721 aagacttaaa tgataatata gtatttatat tccaaccagc agaagaaggt cctgggggag
     9781 caaaagcttt aatagatgaa ggagtgctta gaaaatacaa tattgatgaa atatatgggc
     9841 ttcatatata tccagagtta ccagaaggtt atgtaggtac aagagaaggc tattttatgg
     9901 ctcaaatagg agatatcgac atagaaatag tgtctaaaag tggacatggg gctatgccac
     9961 aaaatggtat tgatggtata gtgatagctt caaactttgt aagctcactt caaactatag
    10021 tgtctagaaa tataagccca atagacaatg cagtgttgac tattggtaga attgaagggg
    10081 gagctagaag aaatataata gctgaaaata taaagatgga aggtactatg agatgtttca
    10141 gcccagaagt gtatgagaaa atgaagctta gagttagaga acttgctaga gggtttgaat
    10201 tggcttataa ctgtaaggtt aatataaata ttatagatga ttatattgct gttaaaaatg
    10261 acaaggattt gtatcaagag tttgtagagg ctattggaag tgatactgtt gtagagttag
    10321 agccacttat gatatcagaa gatttttctt attatcaaaa agaagttcca ggattatttt
    10381 ttatgttggg ttcaagaaac gaagaaaaag gatttgtaaa tggtcttcat aatataaact
    10441 ttaattttga tgaaaagata tgtgtaaatg cattgaatgt ttattcaaaa ttattaaaat
    10501 acaaagaagc aatagattag gaagataaat tataaagatt aaaaagcata aatacctaaa
    10561 tttaaaaaaa atgtattaat aaatacccct tatatatttg tataaattta ttttaattga
    10621 caaaaataca aataagaagg ggtgattttt ttgaaagaaa aagtacaaag agaaaagaag
    10681 ataactaatg gcaagaatac taaaatattg acaaatgatg atataatgaa atatgagata
    10741 gcctcagaac taggtcttat ggataaagtt ggtgaacgtg gatgggcagg acttacagct
    10801 aaggaagcag gaagaattgg aggtatgctt acatcacgca aaaagaggct taaaaagatt
    10861 gtggaggagc agaataaaaa ggatgagtca atatagtgat tttgcgtttg tatatgatga
    10921 acttatgaat gaagttgatt ataatggttg ggttaaatat atagaagata taatagatag
    10981 tgaaggtgta aaagtaaaaa atatattgga attggcatgt ggcacgggta atcttacaat
    11041 tccattgact aaaaaaaatt acgatatagc tggtatagat atatctgatg agatgcttag
    11101 tgtagcaaga gaaaaggcag aaaaagaagg agtagaatta gttttacttc aacaagatat
    11161 atcagagctt gattttgaaa tatctgattt ggactgtgtg ctttgtgctt gtgatggatt
    11221 taattatata acttatgatg atgatttgga aaatgtattt tcaaagacat atgaattatt
    11281 aaagaaagat ggaatattca tatttgatat aagttctttt tataaattag ccaacatatt
    11341 gggaaataac atgtatggag aaaatagaga agatatagcg tatatgtggc aaaattactt
    11401 tgatgaagaa gagaatctaa ttgaaatgga acttacattc tttattaaag atgaatgtgg
    11461 aaagtttgaa agatttgaag agatacacca acaaagagca tatactgaag atgagatagt
    11521 tgatatgctt gaaaaatctg gatttaagga tataaaatat tatggggatt ttacatttga
    11581 caaacccagt gaagaaagtc aaagaatctt ctttgtatgt aaaaaataag atatttttga
    11641 ctatataaga taattattaa aaataaccat gatataaaaa tatagaaaat gcagttttat
    11701 agattaaaag taaagtgatg atactttatg acttttattt tctaataatt ataaagttct
    11761 atagataaaa attaaaatat aataactatt gcttagtaaa attaaaataa aatgggtttt
    11821 gcccaaggtt aatatagata agtacttata gattaaaatt aaggtagtaa tataataaaa
    11881 ataagacaat taacgggaca tggaggaata ttaatgagag attatgtact aagagcgaca
    11941 tcaggaaatg gtcaagttag agcatttgtc gcaactacta gaaatacagt agaagaagct
    12001 agaagattac atgaaactac aaaagtagca acagcagcac ttggaagaac attaacagcc
    12061 acatctataa tgggattaat gatgaaaaat gatagtgata aattgactgt aataataaaa
    12121 ggtggaggtc caattggaac tattatcgcc acatcagatt ctaagggaat ggtaaaagga
    12181 tatgtaggaa atccacaagt agaggttgaa gactatccaa atggaaagtt aaatgtagca
    12241 gcagcagtag gtacagaagg tgttgtaaaa gttataaaag acttaggtct tagagaacca
    12301 tataatggga cttatccatt ggtaagtgga gaaattgcag aagactttac ttattatttt
    12361 gctgtatcag agcagactcc atcagtagta gctttaggtg ttcttacaaa agaagatgaa
    12421 gtagagtttg ctggaggatt tatagttcaa ttaatgccag atgcagaaga agagactatt
    12481 gctaagttag aggaaaatgt agctaaatta ccttctataa ctaacatgtt aaaagaagga
    12541 aaatcaccag atgatatatt aaatattgtt ttggatggtt tggaacctaa aatacttgat
    12601 acctgtgaag tagggtttat gtgtgaatgt tcaaaagaaa gagtaaaaac tgctttggtt
    12661 gcaattggta aaaaatcatt ggctcagata atagaagaag ataaaaaagc agaagttggt
    12721 tgccagttct gtaataagaa gtatatgtat agtgaagaag agttgcttga aattttaaag
    12781 gaaatgtagg ctataaaaat agttgtattg atataagtaa tgttatagtg atatagtaaa
    12841 gtcgtattga tatagtaatt aaagtataat ttattaataa tagacatatt tatattaaaa
    12901 aataaattta ataaattttt aagcttaata aattttaaga attaagaagc tttctggctg
    12961 taatatttgc aatgttgcag ttagaaggct tttttatttt attatagttc gtgtcaaaga
    13021 ttgtatgaaa caataaaata ttgtaaaata atttgtatat ttagacctga ttgacacaaa
    13081 ggtttataag atatttgagt tgattatacg aaattctatg aagtagtaac cctgtgactt
    13141 taatcatggg aggttcagaa gaaggagaaa attttaaata tggaaagtaa aacgaagtac
    13201 ataggaataa gtatattaat attattgatg atatttttaa ttgcaaagaa ttttaacgga
    13261 aaacttgaaa tacctattcc tgagagtaat agtagaaaat ttaaatcagc agcagtgttt
    13321 tatcctcaac atcaagatga tgaggtttta tggggaggaa gtgccattgt agatgctata
    13381 aaacagtgtg gtgttgataa tgtatatgtg gtattagtat cagatggttc tggagttaac
    13441 gttttcaaag ctaacacaaa atttagaaac ttaactagga agcaaaagga agagcttaga
    13501 aataatgagt ttaaatcagc tttgagagag cttggagtga aacctcaaaa tgtgattata
    13561 cttgcagata tagataaaaa ggaaggcaca cattatgaat taatggaaaa gactatattg
    13621 gattttgaac ataagtttaa aggcaatgtg acccatatag cccatcacta taagtatgat
    13681 gaccatataa tgcatagaaa gaatggggaa gttttaaaga aacttaaacg tgaaggtaag
    13741 ataaaagatg acttgtattt tgttaagcca aaatatgtaa agtatatacc aagaaatgaa
    13801 agagctatct ataaagcaat ggattttgat gactacaata aggttaagag tgcttgttat
    13861 gaatataagg ttgtaaattc aaagcgtaaa atgtatggta taggatatat ttcggcacat
    13921 agttattttg aacatcttct aaatgaccct gaacttacgt ctgtattaag tgaagattaa
    13981 tttcttataa gtagtaagtt atgtttgata agttgattgg aattttctat agttttgaag
    14041 gtattggaaa tttagtttta tttaaagatt taaagataga ggtttttaat gtttaaaatt
    14101 ttaaagttaa gttgaataat tgtatagaat tatttgtgaa agaagaaaca gtgtaaaaca
    14161 tagtatctta tttatggttt taaaatcata ggcaagatac tttttttatg taactaatac
    14221 cattttgagg ttattaactt aagaatttta tataatttaa cactcatata taatgatgat
    14281 atttaggtag aatagatgca attacgctta aataaaataa ctgtattttt tataaaaatc
    14341 aaaccctaca ctatactacc agataaaatc tacatgctat aatttgacct gtgtttataa
    14401 aaaaaggagt aatctaataa aatagtattt cacttatgta taactaacaa ctattattac
    14461 gattttctct aaataattta aagaaaaata atgcaggagg taaaatatga gcaatgaatt
    14521 tataatttta aaaggttgca aagagaataa tttgaacaat atttccttaa aaataccaaa
    14581 aagaaaaata accatattca caggggtgtc gggttctgga aagtcatcta ttgtatttga
    14641 gacaattgca aaagaatcac aacgccaact aaatgaaagg tttagtacat ttgtaagaag
    14701 ttttttacct aagtatggag aggttaaagc tgattgtata gaaaatctgt caacaccaat
    14761 tatcatagac caaagtagac ttggtggaaa ttcaaggtca accctaggta ctattacaga
    14821 tgtaaattcc tttttaagag ctttatattc aaggtttggt agtgagtata taggtaatgc
    14881 taatatgttt tcttttaatg atattgatgg tatgtgccca cactgtcatg ggcttggaaa
    14941 aaagctagtt cctaatatga accaaattct tgatatgaat aaatcattaa atgaaggtgc
    15001 tatattgctt tcagggtttg gagtgggttc ttggcattgg aatatattcg ctgaatcagg
    15061 attttttgat attaataaga ggatatgtga ttattcagaa gtagaattgg aaaagttttt
    15121 gcatggtaaa gcagaaaaga ttaagataga aaatgctggt caaactaaca tgacatatga
    15181 gggattaatg gttaaattta atcgtctata cttaagtaga gatggagaaa tatctgaggc
    15241 tactaagaaa aaattgagta aacttcttat agaagataga tgtcctatat gtgatggaag
    15301 aagacttaat gaaggagtat ataggtcttt aattaatggt tataatatag cagatttaac
    15361 tagtatgcaa attgatgaat tggctgaagt tattaaaagt ataaatgagc cagaagcaca
    15421 acctttaatt aaaggaataa tagaaaagtt aaacagtatc atagatatag gtcttggata
    15481 tttaacactg gatagagaga catcttctct atcaggaggg gaatctcagc gtataaagat
    15541 ggttaagtat ttaaatagta atcttgttga tttaatgtat atttttgatg aaccaagtgt
    15601 aggacttcat ccaaaagatg tgtataaatt aaataatctt ttaaaaaaat tgcgtgataa
    15661 agggaataca attatagttg ttgaacatga cccagatgtt attaagattg cagaccatgt
    15721 tgtagatgta ggaccaaagg caggtaagta tggtggaaaa gttgtgtatg aaggcagtta
    15781 taaaaatcta cttacaagtg gtactttgac agggaatgct ttaagtaaat ttcttcctat
    15841 taaagaaagt gtaagagaac atagtggata tttagaaatt aaaaattgta ataaaaacaa
    15901 tttaaaaaat gtatctatta agatacctaa aggtgtattg actctagtta ctggagtagc
    15961 aggagcaggt aaaagtacgt tgattaagga tgaattttta aagcaaaatc ctagtgctgt
    16021 acttatagac caaagccctg tttcagcaaa ttcaaggtcg agtttggcaa catatagtgg
    16081 aattatgaac aacataagaa gcatattttc aaaaacaaat ggcgtaaatg cttctttatt
    16141 cagttcaaat tcagaaggag cttgtgataa ctgtaagggt tctggaatag tagaaatgaa
    16201 tcttgctttt atggagagta taaagagtac ctgcaatgtt tgtgaaggta aaaaatttaa
    16261 aaaagaagtt ttagaatata ggtttcaaag gaagaatata attgaagttc ttgaaatgtc
    16321 tgttttagac gcaattgagt tttttaattt aaaacagatt aaaacgaaat tacaatgtat
    16381 tgaaaaaatg ggtattggat accttacttt aggacaaact cttgatactt tgtctggagg
    16441 agagtgtcaa agattaaagc ttgctagtga attaaataag gaaagttcag tgtatatatt
    16501 agatgaacca acaacaggtc ttcatatggc tgatattgag aattttataa gtataattga
    16561 agagattgta gataatggaa atacggtcat aataattgaa cacaatatag atgtaattaa
    16621 aagagctgat tggattattg agctgggacc tgaaggaggg acaaaaggcg ggagagtaat
    16681 atttgaaggt atcccaaaac aattatgtaa caataaaata tcattaacag caaaatatat
    16741 agtataataa ataaaatata ataaaattat aatgatattt taataaatat aatgggtata
    16801 taaaatgata attaaactta acagtaataa ataatgtgag aaatattcaa atttaattaa
    16861 ataaagtgtc agaaatttgt ttaaatttta gtattttaac gattctgacc ttttttctta
    16921 tttttatgga gagtatcgaa gatggagtct ctaatagtta cttttttgtg tctacttgca
    16981 agtttgatta aaaaatggct atacacactt gtgacttcag tcatgggagg ttcagattat
    17041 aatcttaata tataatttgt gaaataattt gatgacgatt tgctgatgta taaacaataa
    17101 agattaacta cattaaaatt taaatcttgt taagtaaaac ttataaaagg agagagatgg
    17161 atgataaatg atattctaac cttattatct gaaagaagtg atttttttat aaagctatcc
    17221 atagaacatt tgatgatttc tattatttct ataataattg cgattgtttt gggtggtata
    17281 attggtatta ttgtaagtga atttcataat tcttctaaac ctatattggg aactatcaat
    17341 tttctataca ccattccatc tatttctatg ctaggatttc tgataccatt ttccggaatt
    17401 ggaaatacta ctgctgttat tgcacttacc atttatgcat tattgccaat ggttagaaat
    17461 acatatactg gaattactaa tgtggataaa aatattcttg aagcagctag gggaatgggc
    17521 agtacagatt ttcagatatt gtataaaata aaacttccat tagccctacc tgttattatg
    17581 tctggtatta gaaatatggt gattatgaca attgcattag ctggtatcgc ttcatttata
    17641 ggtgctggag gcctaggtgt agccatatat agaggaatca cgacaaataa cacagcaatg
    17701 acaattagtg gaagtatact tattgctcta ttagctttaa tatttgattt tattttaggg
    17761 ctggtagaaa aattttttca acgaaaaaga aaaaagaaaa ataggaaaaa agtttttgcc
    17821 attctaggaa gccttattct tatagcaact atctttacgt cttatttatt taatccttca
    17881 aagactcaaa ctattcatat tgctactaaa cctatgactg aacaatatat tcttggtgag
    17941 atgttggata ttttaattga aaaaaacaca ggtctaaacg tggaaattac acaaggcata
    18001 ggagggggaa catctaatat ccagccagct atggagaatg gagaatttga catttatcca
    18061 gagtatacag gtacaggctg gaatatggta cttaagaaaa atgaattata tactgaaagt
    18121 atgtttagta aactacagtc ggaatacaaa actaaactta aaatgcaatg gactggtatg
    18181 tatggttttt ctaatagcta tgcattagca gttagaaaag aagttgcaga taaatataat
    18241 ttaaaaaata tatcagactt aaaaaatgta tcttctaaat tgacatttgg agctgaatat
    18301 gacttttatg agagagagga tggatatact ccattgtgta atacatatgg actgaacttt
    18361 aaagatacag ttgatttaga tattggatta aaataccaag ctatcaatga aggtaagatt
    18421 gacgtaatgc ctatatttac aactgatggt cagtatagtg tttcagatgt tatagttcta
    18481 aaagatgata aaaatttcta cccatcttat caatgtggaa atgttgttcg tttggaagta
    18541 ttagaggaaa atccagaact atcagaggta tttaagatgg ttggagatat tttgactgat
    18601 aaagaaatgg ctaaaatgaa ttatgacgta gaaaacaaca atttagaacc tagagaagtt
    18661 gcttgtgagt ttctgaataa gaaaggtctt ttataaattg gaggaaatat aatgacacct
    18721 attatacaat ttaaaaatat taaaaaacaa tataatgata aaactataat agataatctt
    18781 aatttagata tagaaaaagg agagtttttg actgtaattg gttcgtctgg ttctggtaag
    18841 acaacactct taaaaatgat taatggtctg attttgccag atggaggaaa tattttgata
    18901 aaccaaacag atatcaaaaa cgaggactta attaaattaa gaagaagaat aggatattgt
    18961 gtacaaggaa gtgttctttt tcctcatatg actgttgaag aaaatatttc atatgtacca
    19021 aatctcctaa gtaaaaagaa taaattagaa gtaaaaagtg ctgtaaacaa gtggatggaa
    19081 atagtgggat tgccaaatga tatgaaagtg cgttacccat cagaattgtc aggtggtcag
    19141 caacaacgtg tagggattgc aagagcttta gcttcttctc cagaaatttt attaatggat
    19201 gaaccttttg gtgcagttga tgagattaca cgaaagcaat tacaaaaaga gataaaagaa
    19261 attcataaaa aaacaggagt tacgattatc tttattacgc atgatattta tgaagctttg
    19321 attttgggaa caaagacttt ggtattaaat catggagttg ttcaacagta tgatactcct
    19381 gagaatattt taaatactcc tgctaaccaa tttgtagacc agttattgaa tataagaaaa
    19441 tctataatag atgatgataa tttaaaagaa taaatttaaa tttaaaatca aattgttgtg
    19501 ataaaaacta ttgatttaga gctaacttca agttgtatac ttgaagtgga aaagttagat
    19561 taaggtatag aagagaattt agcagttggc tgtagacgag gtaactgctg aaggtgcttt
    19621 caataaatgg ctagaacctg tatctattga agattataat aaattgaaat aagaagctca
    19681 atatacagag tagataggca cttcaaatag aagtgtcttt tgtgtgccat aaaataaatc
    19741 aaaatattag caaagaatgt gaaaaacaaa ataaaataat atgctatctt taaaatagga
    19801 ggggtgctat atggataaca atttgattac cattgaaaaa actttagatt atatagaaaa
    19861 acatctttta gatgagatgt tagatttaaa tgtaatttct aaagagatag gctattcaaa
    19921 gtaccattta catagaatgt ttacatcagt tgtcggattt agtatacata attatataca
    19981 aagacgtaga ctaactgaag cagcaagaat gctcattttt acagaaaaac caattattga
    20041 aattgcttta atctcggggt atgacacaca acgttctttt tcaacagggt ttaaaaaaat
    20101 gtttaagcat agccctaagt tttttagaaa acatcaagat tttttaccac tacaattaaa
    20161 atttgatatt aataatagaa aaaggttaca tggaggtatg gttttagatg ttaaaaaagt
    20221 taattgtaca gatataaata ttgttggcta ttctggtaaa gtaaaaaaag gatttcatgt
    20281 tattggaaaa tgttggaggg atttgcataa aaataaagag ttgatagaaa acaaggtaga
    20341 cttggatttt ttaattggag ttaatgatta tagtaatttt aaagaaattg aaaataatcc
    20401 tacatttaca tatattgcag gtgttcaagt agctactttt ggtaaattcc taatggaatg
    20461 gaaaaattcg tattaccgaa aagtaattat attgttttta cttttattgg gaaaaatgag
    20521 gatagtatgc aaaatgttgt agaatatatc tatcaagaat ggttccttaa ttcaagttgc
    20581 cagtttaatg aaaataattt atatgatttt gtaaaatacg gagaaaaaac tgataagaat
    20641 ggtttgagtg aaattcagtt ttgggttcca atcatttaaa agaagggatt atgtataaag
    20701 taggtggatt tatataggca aagttttata aaagtatgta tttataaata atttatagtc
    20761 tgtaggaaag aaatagtttt actataagat tatgataaaa tacacaatac tatgataaaa
    20821 tagtactaaa taatagattt aattttgcaa aggataaagt gttttaataa aaatgaatgt
    20881 agaaaacgaa ttacttaaaa acctagatag attgcataca acagagttag gcgttgtaag
    20941 aattaagaaa aatctttctt tggagacaaa tgatgtagtg aactggtgta aaactaaaat
    21001 agaatctcca aatgccatta ttaatagaaa gggaaaaaac tggtatataa gtgtatatga
    21061 ttgtattata acagtaaacg ctcacagtta tacaattatt acagctcata aagaaaagaa
    21121 atgaattgaa aaatacaaag gtcaaaagca ttttccactg tttgtagtac ctatgaagaa
    21181 gtatataaaa taatattact tttgaataat aatttaaatt ttggaggttg aaaaaatgaa
    21241 taatactgta aaagaaaaat tggcagagtc actaacttct gatactactg atattttacc
    21301 ttatttgcct tatttattac aagatttttg ggaattagga agttcaccag aacaaatgtt
    21361 acaattattt agaaagcata taccttttaa tgaggataca aaagttttgg atttagcttg
    21421 tggaaaaggt gctgtatctg tgaagcttgc aaaagagttg aatatacata tcaaaggtat
    21481 tgatttgata cctgaattta ttgattatgc gagaagaaag gctattgaat ataaggttga
    21541 tgctttgtgt gattttgaaa tgaatgatgc taatagttct gtaaaaattg agcacgatta
    21601 tgattgtgtg atttttggag ctgtagggga tattttaggt aattataaag aaactataag
    21661 taagctatta cctacaataa aaagtaaggg gttcatttta attgatgatg catatgtagt
    21721 agataaatct acaaatgaac agcttaattt tgagagggtg tatcccactt acaatgagtg
    21781 gtgtatattg ttcaaagaat taggtctgaa actaattgaa tgtataccat gtttagaact
    21841 tgaaaatgat gttgaagagg aaatgaactg gattataact cgtgcaaatg agctaattaa
    21901 aaaatatcct tctcaaaaag atatgtttga acaatatgtt attagccaaa aaaatgaata
    21961 tactgattta cagcaggacc ttgttggagc aacttggatt ttacaaaaag aataagataa
    22021 atattagact agataatcaa tgttgtagtg aattatccca tgtctagtga atataaataa
    22081 cataaaaaac cgtcaagatg aatagtttat tcgtgcttga cggtttttag tatttgttga
    22141 agtaaaattt tatttcttta gtatatcact tttagtcaat ccatttgatt tagcttgagt
    22201 attccattga tgattcccca taagtttata taggaatcta gggaagttag gtgaaataaa
    22261 aatagtactt ttgttggaat catctatatg tatattatca ctaagtatat tttttgaaag
    22321 ttcttttaat gcaattccta aatctttttt aggtccttta cccaaaggta cggattctag
    22381 tgaaccaaac atctccccag ctccaattcc aataccttga acccatgtaa agttgatttt
    22441 tttgcaccaa tgatctatca tttgaattgc caaatgatta tgttcacctt cattgaatcc
    22501 acagttaact attgcataca ctggaatagg ttttttattt tgtgttttta aaaatttttc
    22561 acatttctct aaaaaatgaa gtaaatgaga tggaatacca tctacataaa gaggaaatgc
    22621 tattattaat ctatcacaat taagtatggc attaagagcg tcaggatact tagatgtgct
    22681 atctacttta aatgtaaaaa ctttattatc attttgaata agttgtttta attcagataa
    22741 aaaatactgt gaattatttt tatttacttt tggactacca tttatcatta ctatattcat
    22801 atggattcac ctcttatatc ctgtatattt tcatagaaac aaacagagtg ttcttttata
    22861 ttgaaattta tagcatttcc atttactaat ttcgtagcag tttctttttc agcttctgta
    22921 atacaatctc catagaatat agcttttaaa tttatctgtt tatcatatct gcttttatga
    22981 cgcatcatat tatcttttat tacaaagtat ggaagaatat aattaatgct tttatcaagt
    23041 acgtttttga caaaagggct aaaccctccg taacagcatc tgcttattat aataattcca
    23101 tcggattcat ttataagttt acccatgttg ctgtaataat cttttacaac acattcagaa
    23161 ggagttttaa tccagcaacc aaaacgacca atacatttgt ggatttcttt tgtgtttgga
    23221 agtattttta tattgtcatc aagcttgggt aataactttt ctaattcttt gtcagttaaa
    23281 tcatgtatta aaagcttcat aaagtccatc tccttttatt atgtatgttt atttctaaaa
    23341 attatatctt atagattttt cagtaaatct ttacaccaat taataacaaa attataatag
    23401 tcaactccat aaatcattgt ggcatacata tatgtatatt ggtcaataca atgattttct
    23461 tctacatcaa ttttttgttt acttaattta tttagaagta attctacttc agttattaac
    23521 atttttaagt ttgtttcaat tatttctttg ggtagatact tgtaaaagaa tatattaaca
    23581 aggtggtctt gctttgtttt agaaaaattt ataggtttct ttaaccattc gagaaatact
    23641 tctttaccag tattagttat ggaatatagt ttttttaatt tgctaccatc tataacttcg
    23701 tgatagtgta tatatccttt tgtttctaat cttttaagtg caggatagat acttccaaag
    23761 ctggcgtcaa aaaagtacga ggtactttct gacattattt gttttaagtc gtatccactt
    23821 aattcttttt ccattaaaaa tcctaaaata atgtattcta acatattacc tcctggtatc
    23881 aatcagatat atatcgattc gatatattta ttattacaga taataaatat tacgtcaaga
    23941 tgctttttaa aataagctaa aaagaattag gaattattga taaatatgca aaaaaattaa
    24001 tataaaaggt aattaaatat caatgaagat ttaaaaatag ttaaattaaa gtaagagtct
    24061 aatatataaa atttatagta actttatggt ttaaatataa acttgtgtgc tattgacaaa
    24121 gtataaaatt agcagtaaaa taattgcatg agcaaataga ttattgcgaa cgcaaaaaaa
    24181 tgaggacaat agatattaaa ggagagaata tgagagagct attaaaatgg gtttccatta
    24241 taaatagata caatacaaat tatattgaca ggaaattggc ggatattgga atcaataata
    24301 gccaatattt ttatgtatta cacatatgta ataatccagg tattacacag gacactatat
    24361 ttgagaaaat tcttgtgaat cctagtaata taacgagagc tttggcaaat ttagaaaaag
    24421 aaggctttat aacaagagaa ccaggtgtaa aggataaaag aacatggcat ttatatccta
    24481 cagaaaaatc tttagaatgt tatgatgaaa tactaaaaat aacaaatggg tatgttgagg
    24541 agttgttgag ttcatttgag aaagaagagc aagagttgtt tatgtcaatg ttaaagaaag
    24601 ttgcattttt agctattgat atgaatgaag aagcaaaatt ggaggcgaag gaaaatggaa
    24661 gaaaaatata aggagccatt ggaatatgaa aaggtttcaa agttattaac taagtacgca
    24721 attcctagta ttatagcaat gttagtaagt gctttatata acattgttga ccaaatattt
    24781 attgggcaag gtgttggtgt attgggtaat gctgctacaa atgtcacttt tccactaaca
    24841 acaatatgta ctgcaattgc attattatta ggaattggag gagcgtctaa gtgttcattg
    24901 gaattaggtg ctaaaaattc agaaaaagct acaaaagcag caggaaattc tatatgcttg
    24961 atggctgttt ttggaataag tttatttata gtagtatcaa tatttttaac accactatta
    25021 aaattctttg gctctacatc agaaatacta ccttatgcac acacatatac aaaaattaca
    25081 tcaattggat taccatttct tataatgtcc actggaatga gtaaattgat acttgcagat
    25141 gggagaccaa aagcttctat gttttgtatg ttaataggag cagttattaa tacaattttg
    25201 aatccattat tcatttttgt ttttaatatg ggaattgctg ggtctgcttt agcaactgtt
    25261 attgggcaac ttatatcttt tggaataagt gttcattata taaagaattt tcaacatatt
    25321 aaattacata agaaaagttt taaattagat agtataattt ataaacatat atttgcgcta
    25381 ggttcaagct catgttttaa tcaattagca atgactgtaa cacagatagt tatgaataat
    25441 acattggcat attatggggc taggtcagtt tatggaagtg agattccact tgcttgtgtc
    25501 ggtatcatta ttaaagtaaa tatgattttt atatctataa ttattggtat atcacaaggt
    25561 atgcaaccaa ttatagggta taattatggg gctgaaaagt ataatcgtgt aaaagaagca
    25621 tataagtttg cagtgggaag tgcaactatt atatctgtta ttgcattctt atgtttccaa
    25681 ttcttcccaa gacaaattac gtctatattt ggtaacggaa atgagactta ttttatattt
    25741 gcagaacatt attttagaac tttcttgttc ttaacattta taaatggtgt tcaaatagta
    25801 tcatcaactt tctttacatc aattgggaaa tcagtgttgg gactttttac ttctttgact
    25861 cgtcaaattt tatttttaat tccattgata gtaattttac caatgtttat gggaattgat
    25921 ggagtaatgt ttgcaggacc aatagcagat gcagcagcgg caatggtatg tatatacttt
    25981 gcagttcatg aaatgaaatc cttgactata aagcaaaata atttgatgag taaccataat
    26041 aagattgcag aggtataaga caatatttaa gaagtataga aaattaattt aagaaatata
    26101 ataaactaat ttaggaagtg tagaaaatta atttaaagaa gtataataaa ctaatttagg
    26161 aagtgtagaa aattaattta aagaagtata ataa
//
LOCUS       DAELXX010000054        26076 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.054, whole genome shotgun sequence.
ACCESSION   DAELXX010000054 DAELXX010000000
VERSION     DAELXX010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26076
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.054"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            129..683
                     /gene="tcdR"
                     /locus_tag="KPH29_002071"
     CDS             129..683
                     /gene="tcdR"
                     /locus_tag="KPH29_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HBG1467662.1"
                     /translation="MQKSFYELIVLARNNSVDDLQEILFMFKPLVKKLSRVLHYEEGE
                     TDLIIFFIELIKNIKLSSFSEKSDAIIVKYIHKSLLNKTFELSRRYSKMKFNFVEFDE
                     NILNMKNNYQSKSVFEEDICFFEYILKELSGIQRKVIFYKYLKGYSDREISVKLKISR
                     QAVNKAKNRAFKKIKKDYENYFNL"
     gene            1014..8114
                     /gene="tcdB"
                     /locus_tag="KPH29_002072"
                     /gene_synonym="toxB"
     CDS             1014..8114
                     /gene="tcdB"
                     /locus_tag="KPH29_002072"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HBG1467663.1"
                     /translation="MSLVNRKQLEKMANVRFRVQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTIVESATNDTLESFREN
                     LNDPRFDYNKFYRKRMEIIYDKQKNFINYYKTQREENPDLIIDDIVKIYLSNEYSKDI
                     DELNSYIEESLNKVTENSGNDVRNFEEFKGGESFKLYEQELVERWNLAAASDILRISA
                     LKEVGGVYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMVKLEAIMKYKEYIPGYTSEH
                     FDMLDEEVQSSFESVLASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKD
                     SYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNAFIDSIMAEANADNGRFMMEL
                     GKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEISKTNI
                     SQSTEQEMASLWSFDDARAKAQFEEYKKNYFEGSLGEDDNLDFSQNTVVDKEYLLEKI
                     SSLARSSERGYIHYIVQLQGDKISYEAACNLFAKTPYDSVLFQKNIEDSEIAYYYNPG
                     DGEIQEIDKYKIPSIISDRPKIKLTFIGHGKDEFNTDIFAGLDVDSLSTEIETAIDLA
                     KEDISPKSIEINLLGCNMFSYSVNVEETYPGKLLLRVKDKVSELMPSISQDSIIVSAN
                     QYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKIIVKSKNLPELS
                     TLLQEIRNNSNSSDIELEEKVMLAECEINVISNIDTQVVEGRIEEAKSLTSDSINYIK
                     NEFKLIESISDALYDLKQQNELEESHFISFEDILETDEGFSIRFIDKETGESIFVETE
                     KAIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDATHEVNTLNAAFFIQSLIEYN
                     SSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLP
                     VIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTAATTAIITSSLGIASG
                     FSILLVPLAGISAGIPSLVNNELILRDKATKVVDYFSHISLAESEGAFTSLDDKIMMP
                     QDDLVISEIDFNNNSITLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYD
                     VLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEF
                     YWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPVITTEYIREKLSYSFYGSG
                     GTYALSLSQYNMNINIELNENDTWVIDVDNVVRDVTIESDKIKKGDLIENILSKLSIE
                     DNKIILDNHEINFSGTLNGGNGFVSLTFSILEGINAVIEVDLLSKSYKVLISGELKTL
                     MANSNSVQQKIDYIGLNSELQKNIPYSFMDDKGKENGFINCSTKEGLFVSELSDVVLI
                     SKVYMDNSKPLFGYCSNDLKDVKVITKDDVIILTGYYLKDDIKISLSFTIQDENTIKL
                     NGVYLDENGVAEILKFMNKKGSTNTSDSLMSFLESMNIKSIFINSLQSNTKLILDTNF
                     IISGTTSIGQFEFICDKDNNIQPYFIKFNTLETKYTLYVGNRQNMIVEPNYDLDDSGD
                     ISSTVINFSQKYLYGIDSCVNKVIISPNIYTDEINITPIYEANNTYPEVIVLDTNYIS
                     EKINININDLSIRYVWSNDGSDFILMSTDEENKVSQVKIRFTNVFKGNTISDKISFNF
                     SDKQDVSINKVISTFTPSYYVEGLLNYDLGLISLYNEKFYINNFGMMVSGLVYINDSL
                     YYFKPPIKNLITGFTTIGDDKYYFNPDNGGAASVGETIIDGKNYYFSQNGVLQTGVFS
                     TEDGFKYFAPADTLDENLEGEAIDFTGKLTIDENVYYFGDNYRAAIEWQTLDDEVYYF
                     STDTGRAFKGLNQIGDDKFYFNSDGIMQKGFVNINDKTFYFDDSGVMKSGYTEIDGKY
                     FYFAENGEMQIGVFNTADGFKYFAHHDEDLGNEEGEALSYSGILNFNNKIYYFDDSFT
                     AVVGWKDLEDGSKYYFDEDTAEAYIGISIINDGKYYFNDSGIMQIGFVTINNEVFYFS
                     DSGIVESGMQNIDDNYFYIDENGLVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSG
                     LVRVGEDVYYFGETYTIETGWIYDMENESDKYYFDPETKKAYKGINVIDDIKYYFDEN
                     GIMRTGLITFEDNHYYFNEDGIMQYGYLNIEDKTFYFSEDGIMQIGVFNTPDGFKYFA
                     HQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVI
                     SE"
     gene            8236..8736
                     /gene="tcdE"
                     /locus_tag="KPH29_002073"
     CDS             8236..8736
                     /gene="tcdE"
                     /locus_tag="KPH29_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HBG1467664.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAVLLDAMTDEM
                     NAKDEK"
     gene            8815..8976
                     /locus_tag="KPH29_002074"
     CDS             8815..8976
                     /locus_tag="KPH29_002074"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467665.1"
                     /translation="MKLIMKTREQMPRDTQVLNTYNFEASVHYYMDDKVVYQTLVHKD
                     GAWSVGKIY"
     gene            9065..9247
                     /locus_tag="KPH29_002075"
     CDS             9065..9247
                     /locus_tag="KPH29_002075"
                     /inference="COORDINATES: protein motif:HMM:NF022413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467666.1"
                     /translation="MRDEGQNDRENKYQTLINDLIDSLKVIGKIYKEIKTLKKYKICL
                     QITIRQSPYLIEESIN"
     gene            9464..17596
                     /gene="tcdA"
                     /locus_tag="KPH29_002076"
                     /gene_synonym="toxA"
     CDS             9464..17596
                     /gene="tcdA"
                     /locus_tag="KPH29_002076"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021394011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HBG1467667.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YLQLKKLNESIDVFMNKYKNSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEI
                     QNPQFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDET
                     LLESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLAL
                     KNFGGVYLDVDMLPGIHSDLFKTIPRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENF
                     DKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGS
                     YLTNLVIEQVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIA
                     PYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLS
                     QLTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNGVDFNKNTALDKNYLLNNKI
                     PSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLS
                     DDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLD
                     TIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKDSITIG
                     ANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPG
                     LASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVKNIIHNSIDD
                     LIDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVE
                     TEKEIFSKYSEHITKEISTIKNSIITDVNGNLLDNIQLDHTSQVNTLNAAFFIQSLID
                     YSSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEG
                     IPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIG
                     AEVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKEYGPLKTEDDKIL
                     VPIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPYISSHIPSLSV
                     YSAIGIKTENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENNGTKLLDSIRDLYPG
                     KFYWRFYAFFDYAITTLKPVYEDTNTKIKLDKDTRNFIMPTITTDEIRNKLSYSFDGA
                     GGTYSLLLSSYPISMNINLSKDDLWIFNIDNEVREISIENGTIKKGNLIEDVLSKIDI
                     NKNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSYSLLLSGDKNY
                     LISNLSNTIEKINTLGLDSKNIAYNYTDESNNKYFGAISKTSQKSIIHYKKDSKNILE
                     FYNGSTLEFNSKDFIAEDINVFMKDDINTITGKYYVDNNTDKSIDFSISLVSKNQVKV
                     NGLYLNESVYSSYLDFVKNSDGHHNTSNFMNLFLNNISFWKLFGFENINFVIDKYFTL
                     VGKTNLGYVEFICDNNKNIDIYFGEWKTSSSKSTIFSGNGRNVVVEPIYNPDTGEDIS
                     TSLDFSYEPLYGIDRYINKVLIAPDLYTSLININTNYYSNEYYPEIIVLNPNTFHKKV
                     NINLDSSSFEYKWSTEGSDFILVRYLEESNKKILQKIRIKGILSNTQSFNKMSIDFKD
                     IKKLSLGYIMSNFKSFNSENELDRDHLGFKIIDNKTYYYDEDSKLVKGLININNSLFY
                     FDPIESNLVTGWQTINGKKYYFDINTGAASTSYKIINGKHFYFNNNGVMQLGVFKGPD
                     GFEYFAPANTQNNNIEGQAIVYQSKFLTLNGKKYYFDNDSKAVTGWRIINNEKYYFNP
                     NNAIAAVGLQVIDNNKYYFNPDTAIISKGWQTVNGSRYYFDTDTAIAFNGYKTIDGKH
                     FYFDSDCVVKIGVFSGSNGFEYFAPANTYNNNIEGQAIVYQSKFLTLNGKKYYFDNNS
                     KAVTGWQTIDSKKYYFNTNTAEAATGWQTIDGKKYYFNTNTAEAATGWQTIDGKKYYF
                     NTNTSIASTGYTIINGKYFYFNTDGIMQIGVFKVPNGFEYFAPANTHNNNIEGQAILY
                     QNKFLTLNGKKYYFGSDSKAITGWQTIDGKKYYFNPNNAIAATHLCTINNDKYYFSYD
                     GILQNGYITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQN
                     KFLTLNGKKYYFDNDSKAVTGWQTIDSKKYYFNLNTAVAVTGWQTIDGEKYYFNLNTA
                     EAATGWQTIDGKRYYFNTNTYIASTGYTIINGKHFYFNTDGIMQIGVFKGPDGFEYFA
                     PANTHNNNIEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVAV
                     TGWQTINGKKYYFNTNTYIASTGYTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPAN
                     TDANNIEGQAIRYQNRFLYLHDNIYYFGNDSKAATGWATIDGNRYYFEPNTAMGANGY
                     KTIDNKNFYFRNGLPQIGVFKGPNGFEYFAPANTDANNIDGQAIRYQNRFLHLLGKIY
                     YFGNNSKAVTGWQTINSKVYYFMPDTAMAAAGGLFEIDGVIYFFGVDGVKAPGIYG"
     gene            complement(17931..18491)
                     /locus_tag="KPH29_002077"
     CDS             complement(17931..18491)
                     /locus_tag="KPH29_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin regulator"
                     /protein_id="HBG1467668.1"
                     /translation="MFFGNISSPACSEDHEEVISNQTSVIDSQKTEIETLNSKLSDAE
                     PWFKMKDDEKKAIEAENQRKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYK
                     YKKVKFEGKVIQVIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGI
                     SAGTITYESTMGGKITIPGIAVEKIN"
     gene            19028..19273
                     /locus_tag="KPH29_002078"
     CDS             19028..19273
                     /locus_tag="KPH29_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pathogenicity locus"
                     /protein_id="HBG1467669.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLM
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
     gene            complement(19490..19759)
                     /locus_tag="KPH29_002079"
     CDS             complement(19490..19759)
                     /locus_tag="KPH29_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG1467670.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIGQCRKWLEETVSN"
     gene            complement(19971..20273)
                     /locus_tag="KPH29_002080"
     CDS             complement(19971..20273)
                     /locus_tag="KPH29_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467671.1"
                     /translation="MKYSKGFKLIYSLVEYSKKFKVIYSVMKYSKGFKLIYSLVEYSK
                     RFKLIYDVMKYSKEFKLIYSLVEYSKRFKVIYSVMKYSKRFKAIYNAVKYIQGDLK"
     gene            complement(20793..21530)
                     /locus_tag="KPH29_002081"
     CDS             complement(20793..21530)
                     /locus_tag="KPH29_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1467672.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
     gene            complement(21542..22303)
                     /locus_tag="KPH29_002082"
     CDS             complement(21542..22303)
                     /locus_tag="KPH29_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1467673.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFVAVEIMWCIVNSNRAITRNPD
                     MLGGFEYSYMLMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPILMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            complement(22303..23226)
                     /locus_tag="KPH29_002083"
     CDS             complement(22303..23226)
                     /locus_tag="KPH29_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1467674.1"
                     /translation="MKHMEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGA
                     GKSTTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENM
                     TIMQRLLDLPKKNVNEALKIVRLENQKNKKVNQYSLGMKQRLGIAMAIMKFPKLLILD
                     EPTNGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQD
                     SIKELHNRGQSQIAIKAKNVVSAQKLLNTKGYMSLIQNGYLTIENLKDSDVAKINGML
                     VHSNIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            23388..24095
                     /locus_tag="KPH29_002084"
     CDS             23388..24095
                     /locus_tag="KPH29_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1467675.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     RVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKDGQEIS
                     LTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PKHLITVRGLGYKLMIN"
     gene            24140..25546
                     /locus_tag="KPH29_002085"
     CDS             24140..25546
                     /locus_tag="KPH29_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1467676.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLILKDGKFILDDSSKEVLHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTIKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGVKIRELVNDLNLVSKLEYNMQALNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVELDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKSQNGLSEAYAKGQNHGLGILIVAGIINAHRGKLDI
                     MSIDSQGLKTILKLPLIK"
BASE COUNT        10195 a         3057 c         3941 g         8883 t
ORIGIN      
        1 tataatgcgt attttcatat aaaatttaat ttatttgccg attatataat tataatgact
       61 gatttaattc caatgttgtc aaaattttta aataaatcat cattataaat ataagagagg
      121 atgattttat gcaaaagtct ttttatgaat taattgtttt agcaagaaat aactcagtag
      181 atgatttgca agaaatttta tttatgttta agccattagt aaaaaaactt agtagagttt
      241 tacattatga agagggagaa acagatttaa taatattttt tattgaatta ataaaaaata
      301 ttaaattaag tagcttttca gaaaaaagcg atgctattat agtcaaatat attcataaat
      361 cattactgaa taagactttt gagttgtcta gaagatattc taaaatgaag tttaattttg
      421 tagaatttga tgaaaatatc ttaaatatga aaaataatta tcaaagtaag tctgtttttg
      481 aggaagatat ttgttttttc gaatatattt tgaaagaatt atctggtatt caaagaaaag
      541 ttatttttta taaatattta aaaggatatt ctgatagaga aatatcagtg aaattaaaaa
      601 tatctagaca agctgttaat aaggctaaaa atagagcatt taaaaaaata aaaaaagact
      661 atgaaaatta ttttaacttg taattaatga atttaaagaa atatttacaa tagaaatcaa
      721 attttagaat taactttatt gtaaaatcaa taacttaatc taagaatatc ttaattttta
      781 tattttatat agaacaaagt ttacatattt atttcagaca acgtctttat tcaatcgaag
      841 agcaaattaa tcaactgagt gtcttcaatt taaaatgtta ggaagtgaat gtatatgaaa
      901 acctaagtag atattagtat attttataaa tagaaaggag gatatataaa agagttttag
      961 catttagatg taaaaatatt caataaaaat attatagtaa aggagaaaat tttatgagtt
     1021 tagttaatag aaaacagtta gaaaaaatgg caaatgtaag atttcgtgtt caggaagatg
     1081 aatatgtagc aatattggat gctttagaag aatatcataa tatgtcagag aatactgtag
     1141 tcgaaaaata tttaaaatta aaagatataa atagtttaac agatatttat atagatacat
     1201 ataaaaagtc tggtagaaat aaagccttaa aaaaatttaa ggaataccta gttacagaag
     1261 tattagagct aaagaataat aatttaactc cagttgagaa aaatttacat tttgtttgga
     1321 ttggaggtca aataaatgac actgctatta attatataaa tcaatggaaa gatgtaaata
     1381 gtgattataa tgttaatgtt ttttatgata gtaatgcatt tttgataaac acattgaaaa
     1441 aaactatagt agaatcagca acaaatgata cacttgaatc atttagagaa aacttaaatg
     1501 accctagatt tgactataat aaattttaca gaaaacgtat ggagataatc tatgataagc
     1561 agaaaaattt cataaattac tataaaactc aaagagaaga aaatcctgac cttataattg
     1621 atgatattgt aaagatatat ctttcaaacg agtattcaaa ggatatagat gaacttaatt
     1681 cctatattga agagtcatta aataaagtta cagaaaatag tgggaatgat gttagaaact
     1741 ttgaagaatt taaaggtgga gagtcattca aattatatga acaagagttg gtagaaagat
     1801 ggaatttggc agctgcttct gacatattaa gaatatctgc cttaaaagaa gttggtggtg
     1861 tatatttaga tgttgatatg ttaccaggaa tacaaccaga cctatttgag tctatagaga
     1921 aacctagttc agtaacagtg gatttttggg aaatggtaaa attagaagct ataatgaaat
     1981 acaaagaata cataccagga tatacttcag agcattttga tatgttggat gaagaagttc
     2041 aaagtagttt tgaatctgtt ctagcttcta agtcagataa gtcagaaata ttctcatcac
     2101 ttggcgatat ggaggcatca ccactagaag ttaaaattgc atttaatagt aaaggtatta
     2161 taaatcaagg actaatttct gtgaaagact catattgtag caatttaata gtaaaacaaa
     2221 tcgagaacag atataagata ttgaataata gtttaaatcc agctattagc gaggataacg
     2281 atttcaatac tacaacgaat gcttttattg atagtataat ggctgaagct aatgcagata
     2341 atggtagatt tatgatggaa ctaggaaagt atttaagagt tggtttcttc ccagatgtta
     2401 aaactactat taacttaagt ggccctgaag catatgcggc agcttatcaa gatttattaa
     2461 tgtttaaaga aggtagtatg aatatccatt tgatagaagc tgacttaaga aactttgaaa
     2521 tctctaaaac taatatttct caatcaactg aacaagaaat ggctagctta tggtcatttg
     2581 acgatgcaag agctaaagct caatttgaag aatacaaaaa aaattacttt gaaggttctc
     2641 ttggagaaga tgacaatctt gacttttctc aaaatacagt agttgacaag gagtatcttt
     2701 tagaaaaaat atcttcatta gcaagaagtt cagagagagg atatatacac tatattgttc
     2761 agttacaagg agataaaatt agttatgaag cagcatgtaa cttatttgca aagactcctt
     2821 atgatagtgt actgtttcag aaaaatatag aagattcaga aattgcatat tattataatc
     2881 ctggagatgg tgaaatacaa gaaatagaca agtataaaat tccaagtata atttctgata
     2941 gacctaagat taaattaaca tttattggtc atggtaaaga tgaatttaat actgatatat
     3001 ttgcaggtct tgatgtagat tcattatcca cagaaataga aacagcaata gatttagcta
     3061 aagaggatat ttctcctaag tcaatagaaa taaacttact gggatgtaac atgtttagct
     3121 attctgtaaa tgtagaggag acttatcctg ggaaattatt acttagagtt aaagataaag
     3181 tatcagaatt aatgccatct ataagtcaag actctattat agtaagtgca aatcaatatg
     3241 aagttagaat aaatagtgaa ggaagaagag aattattgga tcattctggt gaatggataa
     3301 ataaagaaga aagtattata aaggatattt catcaaaaga atatatatca tttaatccta
     3361 aagaaaataa aattatagta aaatctaaaa atttacctga attatctaca ttattacaag
     3421 aaattagaaa caattctaat tcaagtgata ttgaactaga agaaaaagta atgttagcag
     3481 aatgtgagat aaatgttatt tcaaatatag atacacaagt ggtagaagga aggattgaag
     3541 aagctaaaag cttaacttct gactctatta attatataaa gaatgaattt aaactaatag
     3601 aatctatttc tgatgcacta tacgatttaa aacaacagaa tgaattagaa gagtctcatt
     3661 ttatatcttt tgaggatata ttggagactg atgaaggctt tagtataaga tttattgata
     3721 aagaaactgg agaatctata tttgtagaaa ctgaaaaggc aatattctct gaatatgcta
     3781 atcatataac tgaagagatt tctaagataa aaggtactat atttgatact gtaaatggta
     3841 agttagtaaa aaaagtaaat ttagatgcta cacatgaagt gaatacttta aatgctgcat
     3901 tttttataca atcattaata gaatataata gttctaaaga atctcttagt aatttaagtg
     3961 tagcaatgaa agttcaagtt tatgctcaat tatttagtac tggtttaaat actattacag
     4021 atgcagccaa agttgttgaa ttagtatcaa ctgcattaga tgaaactata gatttacttc
     4081 ctacattatc tgaaggatta cctgtaattg caactatcat agatggtgta agtttaggtg
     4141 cagcaatcaa agagctaagt gaaacaagtg acccattatt aagacaagaa atagaagcta
     4201 agataggtat aatggcagta aatttaacag cagctacaac tgcaatcatt acttcatctt
     4261 taggaatagc tagtggattt agtatacttt tagttccttt agcaggaatt tcagcaggta
     4321 taccaagttt agtaaacaat gagcttatcc taagagataa agcaacaaaa gttgtagatt
     4381 attttagtca tatatcatta gctgagtctg aaggagcatt tacttcatta gatgataaaa
     4441 taatgatgcc acaagatgat ttagtaatat ctgagataga ctttaataac aattcaataa
     4501 ctttaggtaa atgtgaaatc tggagaatgg aaggtggctc aggtcatact gtaactgatg
     4561 atatagatca cttcttttca gcaccatcaa taacatatag agagccacac ttatctatat
     4621 atgacgtatt ggaagtacaa aaagaagaac ttgatttgtc aaaagattta atggtattac
     4681 ctaatgctcc aaatagagta tttgcttggg aaacaggatg gacaccaggt ttaagaagct
     4741 tagaaaatga tggcacaaaa ctgttagacc gtataagaga taactatgaa ggtgagtttt
     4801 attggagata ttttgctttt atagctgatg ctttaataac aacattaaaa ccaagatatg
     4861 aagatactaa tataagaata aatttagata gtaatactag aagctttata gtcccagtga
     4921 taactacaga atatataaga gagaaattat catattcttt ttatggttct ggaggaactt
     4981 atgcattatc tctttctcaa tacaatatga atataaacat agaattaaat gaaaatgata
     5041 cttgggttat agatgtcgac aatgtcgtaa gagatgtcac tatagaatct gataaaatta
     5101 aaaaaggaga tttaatagag aatattttat ctaaattaag tattgaagac aataaaatta
     5161 ttttagataa tcatgaaatt aatttctctg gaacattaaa tggaggtaat ggatttgtat
     5221 ctttaacatt ctcaatctta gaaggaataa atgcagttat agaagttgat ttattatcta
     5281 aatcatataa agttcttatt tctggtgaac taaaaacatt gatggcaaat tcaaattctg
     5341 ttcaacagaa aatagattat ataggattga acagcgaatt acaaaaaaat ataccttata
     5401 gttttatgga tgataaagga aaagaaaatg gatttattaa ttgttctaca aaagaaggtt
     5461 tatttgtatc tgaattatct gatgtagttc ttataagtaa agtttatatg gacaatagta
     5521 aacctctatt tggatattgt agtaatgatt tgaaagatgt taaagtcata actaaagatg
     5581 acgttattat attaacagga tattatttaa aagatgatat aaaaatctct ctttctttta
     5641 ctatacaaga tgaaaatact ataaaattaa atggagtata tttagatgaa aatggagtag
     5701 ctgaaatatt gaaatttatg aataaaaaag gtagtacaaa tacttcagat tctttaatga
     5761 gctttttaga aagtatgaat ataaaaagta ttttcataaa ttccttacaa tctaatacta
     5821 agcttatatt agatactaat tttataataa gtggtactac ttctattggt caatttgagt
     5881 ttatttgtga taaagataat aatatacaac catatttcat taagtttaat acactagaaa
     5941 ctaaatatac tctatatgta ggtaatagac aaaatatgat agtagaacca aattatgatt
     6001 tagatgattc tggagatata tcttcaactg tcattaattt ttctcagaaa tacctttatg
     6061 gaatagacag ttgtgttaat aaagttataa tttcgccaaa tatatataca gatgaaataa
     6121 acataacacc tatatatgaa gcaaataata cttatccaga agtgattgta ttagatacaa
     6181 attatataag tgaaaaaatc aatattaata tcaatgattt atctatacga tatgtatgga
     6241 gtaatgatgg aagtgatttt attcttatgt caactgatga agagaacaag gtatcacaag
     6301 ttaaaataag atttactaat gtttttaaag gtaatactat atcagataag atatctttta
     6361 attttagtga taagcaagat gtatctataa ataaagttat ttcaacattt acaccttcat
     6421 attatgtgga aggattactt aattatgatt taggtctgat ttctttatac aatgagaaat
     6481 tttatattaa taactttgga atgatggtgt ctggattagt atatattaat gattcattat
     6541 attatttcaa gccaccaata aagaacttga taactggatt tacaactata ggtgatgata
     6601 aatactactt taatccagat aatggtggag ctgcttcagt cggagaaaca ataattgatg
     6661 gcaaaaacta ctacttcagc caaaatggag tgttacaaac aggtgtattt agtacagaag
     6721 atggatttaa atattttgct ccagcagata cacttgatga aaatctagag ggggaagcaa
     6781 ttgattttac tggcaaacta actattgatg aaaatgttta ttattttgga gataattata
     6841 gagcagctat agaatggcaa acattagatg atgaagtgta ctattttagt acagatacag
     6901 gtagagcttt taaagggcta aatcaaatag gtgatgataa attctatttc aactctgatg
     6961 gtattatgca aaaaggattt gttaatataa atgataagac attctatttt gatgattctg
     7021 gtgtgatgaa gtcaggatat actgaaatag atggaaaata tttttacttt gctgagaatg
     7081 gagaaatgca aataggagta tttaatacag cagatggatt taaatatttt gctcatcatg
     7141 atgaagattt aggaaatgaa gaaggtgaag cactttcata ttctggtata cttaatttta
     7201 acaataagat ttattatttt gatgattcat ttacagcagt agttggatgg aaggatttag
     7261 aagatggttc aaaatattac tttgatgaag atacagcaga agcatatata ggtatctcaa
     7321 taattaatga tggtaaatat tattttaatg attctggaat catgcaaatt ggatttgtca
     7381 caataaataa tgaagtattt tatttctctg attctggaat agtagaatct ggaatgcaaa
     7441 atatagatga taattatttc tatatagacg aaaatggtct agttcaaatt ggtgtatttg
     7501 acacttcaga tggatataaa tactttgcac cagctaatac tgtaaatgat aatatctatg
     7561 gacaagcagt tgaatatagt ggtttagtta gagttggtga agatgtatat tattttggag
     7621 aaacatatac aattgagact ggttggatat atgatatgga aaatgaaagt gataaatatt
     7681 atttcgatcc agaaactaaa aaagcatata aaggtattaa tgtaattgat gatataaaat
     7741 actattttga tgagaatgga ataatgagaa caggtcttat aacatttgaa gataatcatt
     7801 actattttaa tgaagatggt attatgcaat atggttatct aaatatagaa gataagacgt
     7861 tctactttag tgaagatggt attatgcaga ttggagtatt taatacacca gatggattta
     7921 aatattttgc acatcaaaat actttagatg agaattttga gggagaatca ataaactata
     7981 ctggttggtt agatttagat gaaaagagat attattttac agatgaatat attgcagcaa
     8041 ctggttcagt tattattgat ggtgaggagt attattttga tcctgataca gctcaattag
     8101 tgattagtga atagataaaa atacgttaaa tatatcctct tatacttaaa tatataaaaa
     8161 taaacaaaat gatacactac ataaagtgtt ctatctaata tgaagattta ccaataaaaa
     8221 ggtgaactat gatgaatgca cagtagttca cctttttata tttctaatgg taacaaaata
     8281 tttttttata taaacctagg aggcgttatg aatatgacaa tatctttttt atcagagcat
     8341 atatttataa agttagtaat tttaactata tcatttgata cattattagg atgtttaagt
     8401 gcaataaaaa gtcgtaaatt taattctagt tttggaatag atggaggaat cagaaaagta
     8461 gcaatgatag catgtatatt ttttttatca gtagttgaca ttcttacaaa gtttaacttt
     8521 ttatttatgt taccacaaga ttgtatcaat tttttaagac taaaacatct tggaatatct
     8581 gaatttttct ctattttatt tattttatat gaaagtgtaa gcatattaaa aaatatgtgc
     8641 ttatgtggat taccagtacc taagagatta aaggaaaaaa tagcagtttt actagatgca
     8701 atgacagatg aaatgaatgc taaggatgaa aagtaagtaa tggtagatat aataaaaata
     8761 ttaacaaata aaaagtgtta tccaaataag aatagctgaa agttatcata attcatgaaa
     8821 ctgataatga aaacgaggga gcagatgcca agagacacac aagtattaaa tacatataat
     8881 ttcgaagcaa gtgttcatta ctatatggat gacaaggtag tatatcaaac attggttcac
     8941 aaagatggtg catggtcagt tggtaaaatc tattaagcta cattagttac agatatcaca
     9001 aactataata gttaaacata gaaatatgtg taaattgtga tggaaattat tcaaaaacac
     9061 aaaaatacgt gatgaaggac aaaatgatag agaaaataag tatcaaacct taataaatga
     9121 tttaattgat agtttaaaag ttataggaaa aatatataaa gaaataaaaa cattaaaaaa
     9181 atataagata tgtttacaaa ttactatcag acaatctcct tatctaatag aagagtcaat
     9241 taactaattg agtatcttta aattgaaatg ttaggaagtg atttaaatat gaaaacttaa
     9301 attataaaaa atcaatatta atttatcttt aaaaaataga aaggagtgta taagatttat
     9361 tttcaaagtt taaaaacaag aaaatcaatt taaatttcag aaggaataaa tgtggttata
     9421 gaagtggatt tattatcaaa aataatagta ctaggaggtt tttatgtctt taatatctaa
     9481 agaagagtta ataaaactcg catatagcat tagaccaaga gaaaatgagt ataaaactat
     9541 actaactaat ttagacgaat ataataagtt aactacaaac aataatgaaa ataaatattt
     9601 acaattaaaa aaactaaatg aatcaattga tgtttttatg aataaatata aaaattcaag
     9661 cagaaataga gcactctcta atctaaaaaa agatatatta aaagaagtaa ttcttattaa
     9721 aaattccaat acaagtcctg tagaaaaaaa tttacatttt gtatggatag gtggagaagt
     9781 cagtgatatt gctcttgaat acataaaaca atgggctgat attaatgcag aatataatat
     9841 taaactgtgg tatgatagtg aagcattctt agtcaataca ctaaaaaagg ctatagttga
     9901 atcttctacc actgaagcat tacagctact agaggaagag attcaaaatc ctcaatttga
     9961 taatatgaaa ttttacaaaa aaaggatgga atttatatat gatagacaaa aaaggtttat
    10021 aaattattat aaatctcaaa tcaataaacc tacagtacct acaatagatg atattataaa
    10081 gtctcatcta gtatctgaat ataatagaga tgaaacttta ttagaatcat atagaacaaa
    10141 ttctttgaga aaaataaata gtaatcatgg gatagatatc agggctaata gtttgtttac
    10201 agaacaagag ttattaaata tttatagtca ggagttgtta aatcgtggga atttagctgc
    10261 agcatctgac atagtaagat tattagccct aaaaaatttt ggcggagtat atttagatgt
    10321 tgatatgctt ccaggtattc actctgattt atttaaaaca atacctagac ctagctctat
    10381 tggactagac cgttgggaaa tgataaaatt agaggctatt atgaagtata aaaaatatat
    10441 aaataattat acatcagaaa actttgataa acttgatcaa caattaaaag ataattttaa
    10501 actcattata gaaagtaaaa gtgaaaaatc tgagatattt tctaaattag aaaatttaaa
    10561 tgtatctgat cttgaaatta aaatagcttt cgctttaggc agtgttataa atcaagcctt
    10621 gatatcaaaa caaggttcat atcttactaa cctagtaata gaacaagtaa aaaatagata
    10681 tcaattttta aaccaacacc ttaacccagc catagagtct gacaataact tcacagatac
    10741 tactaagatt tttcatgatt cactatttaa ttcagctacc gcagaaaact ctatgttttt
    10801 aacaaaaata gcaccatact tacaagtagg ttttatgcca gaagctcgct ccacaataag
    10861 tttaagtggt ccaggagctt atgcatcagc ttactatgat ttcataaatt tacaagaaaa
    10921 tactatagaa aaaactttaa aagcatcaga tttaatagaa tttaaattcc cagaaaataa
    10981 tctatctcaa ttgacagaac aagaaataaa tagtctatgg agctttgatc aagcaagtgc
    11041 aaaatatcaa tttgagaaat atgtaagaga ttatactggt ggatctcttt ctgaagacaa
    11101 tggggtagac tttaataaaa atactgccct cgacaaaaac tatttattaa ataataaaat
    11161 tccatcaaac aatgtagaag aagctggaag taaaaattat gttcattata tcatacagtt
    11221 acaaggagat gatataagtt atgaagcaac atgcaattta ttttctaaaa atcctaaaaa
    11281 tagtattatt atacaacgaa atatgaatga aagtgcaaaa agttactttt taagtgatga
    11341 tggagaatct attttagaat taaataaata taggatacct gaaagattaa aaaataagga
    11401 aaaagtaaaa gtaaccttta ttggacatgg taaagatgaa ttcaacacaa gcgaatttgc
    11461 tagattaagt gtagattcac tttccaatga gataagttca tttttagata ccataaaatt
    11521 agatatatca cctaaaaatg tagaagtaaa cttgcttgga tgtaatatgt ttagttatga
    11581 ttttaatgtt gaagaaactt atcctggtaa gttactatta agtattatgg acaaaattac
    11641 ttccacttta cctgatgtaa ataaagattc tattactata ggagcaaatc aatatgaagt
    11701 aagaattaat agtgagggaa gaaaagaact tctagctcac tcaggtaaat ggataaataa
    11761 agaggaagct attatgagcg atttatctag taaagaatac attttttttg attccataga
    11821 taataagcta aaagcaaagt ccaagaatat tccaggttta gcgtcaatat cagaagatat
    11881 aaaaacatta ttacttgatg caagtgttag tcctgataca aaatttattt taaataatct
    11941 taagcttaat attgaatctt ctattggtga ttacatttat tatgaaaaat tagaacctgt
    12001 taaaaatata atccacaatt ctatagatga tttaatagat gagttcaatc tacttgaaaa
    12061 tgtatctgat gaattatatg aattaaaaaa attaaataat ctagatgaga agtatttaat
    12121 atcttttgaa gatatctcaa aaaataattc aacttattct gtaagattta ttaacaaaag
    12181 taatggtgaa tcagtttatg tagagacaga aaaagaaatt ttttcaaaat atagcgaaca
    12241 tattacaaaa gaaataagta ctataaagaa tagtataatt acagatgtta atggtaattt
    12301 attggataat atacagttag atcatacttc tcaagttaat acattaaacg cagcattctt
    12361 tattcaatca ttaatagatt atagtagcaa taaagatgta ctgaatgatt taagtacctc
    12421 agttaaggtt caactttatg ctcaactatt tagtacaggt ttaaatacta tatatgactc
    12481 tatccaatta gtaaatttaa tatcaaatgc agtaaatgat actataaatg tactacctac
    12541 aataacagag gggataccta ttgtatctac tatattagac ggaataaact taggtgcagc
    12601 aattaaggaa ttactagacg aacatgaccc attactaaaa aaagaactag aagctaaggt
    12661 gggtgtttta gcaataaata tgtcattatc tatagctgca acggtagctt caattgttgg
    12721 aataggtgct gaagttacta ttttcttatt acctatagct ggtatatctg cgggaatacc
    12781 ttcattagtt aataatgaat taatattgca tgataaggca acttcagtgg taaactattt
    12841 taatcatttg tctgaatcta aagaatatgg ccctcttaaa acagaagatg ataaaatttt
    12901 agttcctatt gatgatttag taatatcaga aatagatttt aataataatt cgataaaact
    12961 aggaacatgt aatatattag caatggaggg gggatcagga cacacagtga ctggtaatat
    13021 agatcacttt ttctcatctc catatataag ctctcatatt ccttcattat cagtttattc
    13081 tgcaataggt ataaaaacag aaaatctaga tttttcaaaa aaaataatga tgttaccaaa
    13141 tgctccttca agagtgtttt ggtgggaaac tggagcagtt ccaggtttaa gatcattgga
    13201 aaataatggg actaaattgc ttgattcaat aagagattta tacccaggca aattttactg
    13261 gagattctat gcctttttcg attatgcaat aactacatta aaaccagtgt atgaagacac
    13321 taatactaaa attaaactag ataaagatac tagaaacttt ataatgccaa ctataactac
    13381 tgacgaaatt agaaacaaat tatcttattc atttgatgga gcaggaggaa cttactcttt
    13441 attattatct tcatatccaa tatcaatgaa tataaattta tctaaagatg atttatggat
    13501 atttaatatt gataatgaag taagagaaat atctatagaa aatggtacta ttaaaaaagg
    13561 aaatttaata gaagatgttt taagtaaaat tgatataaat aaaaataaac ttattatagg
    13621 caatcaaaca atagattttt caggtgatat agataacaaa gatagatata tattcttgac
    13681 ttgtgagtta gatgataaaa ttagtttaat aatagaaata aatcttgttg caaaatctta
    13741 tagtttgtta ttgtctgggg ataaaaatta tttgatatcc aatttatcta atactattga
    13801 gaaaatcaat actttaggcc tagatagtaa aaatatagct tacaattaca ctgatgaatc
    13861 taataataaa tattttggag ctatatctaa aacaagtcaa aaaagcataa tacattataa
    13921 aaaagacagt aaaaatatat tagaatttta taatggcagt acattagaat ttaacagtaa
    13981 agactttatt gctgaagata taaatgtatt tatgaaagat gatattaata ctataacagg
    14041 aaaatactat gttgataata atactgataa aagtatagat ttctctattt ctttagttag
    14101 taaaaatcaa gtaaaagtaa atggattata tttaaatgaa tccgtatact catcttacct
    14161 tgattttgtg aaaaattcag atggacacca taatacttct aattttatga atttattttt
    14221 gaacaatata agtttctgga aattgtttgg gtttgaaaat ataaattttg taatcgataa
    14281 atactttacc cttgttggta aaactaatct tggatatgta gaatttattt gtgacaataa
    14341 taaaaatata gatatatatt ttggtgaatg gaaaacatcg tcatctaaaa gcactatatt
    14401 tagcggaaat ggtagaaatg ttgtagtaga gcctatatat aatcctgata cgggtgaaga
    14461 tatatctact tcactagatt tttcctatga acctctctat ggaatagata gatatatcaa
    14521 taaagtattg atagcacctg atttatatac aagtttaata aatattaata ccaattatta
    14581 ttcaaatgag tactaccctg agattatagt tcttaaccca aatacattcc acaaaaaagt
    14641 aaatataaat ttagatagtt cttcttttga gtataaatgg tctacagaag gaagtgactt
    14701 tattttagtt agatacttag aagaaagtaa taaaaaaata ttacaaaaaa taagaatcaa
    14761 aggtatctta tctaatactc aatcatttaa taaaatgagt atagatttta aagatattaa
    14821 aaaactatca ttaggatata taatgagtaa ttttaaatca tttaattctg aaaatgaatt
    14881 agatagagat catttaggat ttaaaataat agataataaa acttattact atgatgaaga
    14941 tagtaaatta gttaaaggat taatcaatat aaataattca ttattctatt ttgatcctat
    15001 agaatctaac ttagtaactg gatggcaaac tatcaatggt aaaaaatatt attttgatat
    15061 aaatactgga gcagcttcaa ctagttataa aattattaat ggtaaacact tttattttaa
    15121 taataatggt gtgatgcagt taggagtatt taaaggacct gatggatttg agtattttgc
    15181 acctgccaat actcagaata ataacataga aggtcaggct atagtttatc aaagtaaatt
    15241 cttaactttg aatggcaaaa aatattattt tgataatgac tcaaaagcag tcactggatg
    15301 gaggattatt aacaatgaga aatattactt taatcctaat aatgctattg ctgcagtcgg
    15361 attgcaagta attgacaata ataagtatta tttcaatcct gacactgcta tcatctcaaa
    15421 aggttggcag actgttaatg gtagtagata ctactttgat actgataccg ctattgcctt
    15481 taatggttat aaaactattg atggtaaaca cttttatttt gatagtgatt gtgtagtgaa
    15541 aataggtgtg tttagtggct ctaatggatt tgaatatttc gcacctgcta atacttataa
    15601 taataacata gaaggtcagg ctatagttta tcaaagtaaa ttcttaactt tgaatggtaa
    15661 aaaatattac tttgataata actcaaaagc agttaccgga tggcaaacta ttgatagtaa
    15721 aaaatattac tttaatacta acactgctga agcagctact ggatggcaaa ctattgatgg
    15781 taaaaagtat tactttaata ctaacactgc tgaagcagct actggatggc aaactattga
    15841 tggtaaaaaa tattacttta atactaacac ttctatagct tcaactggtt atacaattat
    15901 taatggtaaa tatttttatt ttaatactga tggtattatg cagataggag tgtttaaagt
    15961 acctaatgga tttgaatact ttgcacctgc taatactcat aataataaca tagaaggtca
    16021 agctatactt taccaaaata aattcttaac tttgaatggt aaaaaatatt actttggtag
    16081 tgactcaaaa gcaattactg gatggcaaac cattgatggt aaaaaatatt actttaatcc
    16141 taataatgct attgctgcga ctcatctatg cactataaat aacgacaagt attactttag
    16201 ttatgatgga attcttcaaa atggatatat tactattgaa agaaataatt tctattttga
    16261 tgctaataat gaatctaaaa tggtaacagg agtatttaaa ggacctaatg gatttgagta
    16321 ttttgcacct gctaatactc ataataataa catagaaggt caggctatag tttaccagaa
    16381 taaattctta actttgaatg gcaaaaaata ttattttgat aatgactcaa aagcagttac
    16441 tggatggcaa actattgata gtaaaaaata ttactttaat cttaacactg ctgttgcagt
    16501 tactggatgg caaactattg atggtgaaaa atattacttt aatcttaaca ctgctgaagc
    16561 agctactgga tggcaaacta ttgatggtaa aagatactac tttaatacta acacttatat
    16621 agcttcaact ggttatacga ttattaatgg taaacatttt tattttaata ctgatggtat
    16681 tatgcagata ggagtgttta aaggacctga tggatttgaa tactttgcac ctgctaatac
    16741 tcataataat aacatagaag gtcaagctat actttaccaa aataaattct taactttgaa
    16801 tggtaaaaaa tattactttg gtagtgactc aaaagcagtt accggattgc gaactattga
    16861 tggtaaaaaa tattacttta atactaacac tgctgttgca gttactggat ggcaaactat
    16921 taatggtaaa aaatactact ttaatactaa cacttatata gcttcaactg gttatacaat
    16981 tattagtggt aaacattttt attttaatac tgatggtatt atgcagatag gagtgtttaa
    17041 aggacctgat ggatttgaat actttgcacc tgctaatacg gatgctaaca acatagaagg
    17101 tcaagctata cgttatcaaa atagattcct atatttacat gacaatatat attactttgg
    17161 caatgattca aaagcggcta ctggttgggc aactattgat ggtaatagat attacttcga
    17221 gcctaataca gctatgggtg cgaatggtta taaaactatt gataataaaa atttttactt
    17281 tagaaatggt ttacctcaga taggagtgtt taaaggacct aatggatttg aatactttgc
    17341 acctgctaat acggatgcta acaatataga tggtcaagct atacgttatc aaaatagatt
    17401 cctacattta cttggaaaaa tatattactt tggtaataac tcaaaagcag ttactggatg
    17461 gcaaactatt aatagtaaag tatattactt tatgcctgat actgctatgg ctgcagctgg
    17521 tggacttttc gagattgatg gtgttatata tttctttggt gttgatggag taaaagcccc
    17581 tgggatatat ggctaaaata tatgtttgac aaaaaattat tcctgtgata ctcagaaatt
    17641 atttttatat aataaatatt gagatttaat taaaagtcat gtgttattgt aatacatgac
    17701 ttttagttaa aatttttcta tcatttaata atctattatt cttgactatt ttataataaa
    17761 attcatatat ggaaatatta atactaaata attaatagtt gatagaaaat agataatatg
    17821 ctaaaagcaa aaactaattt agagccttgt aactgtttat ttgcaattat aaaaacatct
    17881 ttaaacatat tgactataat ataaaatatt aactataata caaaacaata ttaattaatt
    17941 ttctctacag ctatccctgg tatggttatt tttccaccca tagttgattc ataagttata
    18001 gttccagcac ttatacctct tatagttatg taatcatcct ctaacactct tgaaggagtt
    18061 attgattttt tataactaca tagtacgacc ttatcataat ttccagacac agctaatctt
    18121 atttgcacct catcaccatc ttcaataact tgaataacct taccttcaaa ttttaccttt
    18181 ttgtacttat aatcatcagg tgttctagct aattggtcat aagtaatacc agtatcatat
    18241 cctttcttct cttcttcttc ttttttacgt tgttcttcag cctttttagc ttcttcagct
    18301 ttacgttgat tttcagcttc aatagctttc ttttcgtcgt ctttcatttt gaaccatggt
    18361 tcagcatcag acaatttgct atttaaagtt tctatttctg ttttttgaga atctataact
    18421 gatgtttgat tagaaatgac ctcctcatgg tcttcagaac aagctggtga ggatatattg
    18481 ccaaaaaaca caccaaaata aatgccagta gagcaatatc ctttgtgctc ttaaagaatt
    18541 taattatttt cttagagctt ccttttcttt cattactaaa ttcgttaccc tcattttttt
    18601 tagaaaacat aatacaatct cccttttatt agatatattt tattttaaaa tttaaaaact
    18661 taaaataact agttgactaa aaattatata ttcatatttt cctaaaatat atttccccta
    18721 agcataattt tacaaaataa atcaagaaga ttcaatatat tttgaaaaat gaggtaaaaa
    18781 tgtcagatta taatataatt ttcttgaagg tgaattttat atattaaatg aaacataaag
    18841 tattttattt gtataaaact atattatgat gtaattaaat gattcagtac tttagaggtg
    18901 gtaaaatata gatagatgaa agagcataaa taaagtgata taataaaaaa ttaaatatta
    18961 actataataa aaaaatatct ggtactaagt ataatacaaa aataatatat cagaaagaag
    19021 gtgaagtatg ggagaactac aaaaaattcc aggggtaggg aaagcaacag aaaaatcttt
    19081 aatcatgtta ggatatacaa ctattaaatc acttaaagat gcaaacccag cacaaatgta
    19141 tgaaaaggaa tgtttaatgc gtggacagca catagacaga tgtcagttat atgtatacag
    19201 atgtgcagta tattttgcat caactgaaaa tccagaacca gaaaaattaa agtggtggta
    19261 ttggaaagat taaaaaatca aaaaagttag atataaaaaa gagttgaata tccgtttaaa
    19321 taataaaaac taatactcaa ctctttttta gtcgtaaatt ttatgtaaat ctatttgtat
    19381 ggctaaatat acccatcaat aaatgtataa atataccaat caataaatga ctaaacatac
    19441 tacctaacaa aggattaaat aaattactta acaaagcatt ctaaaacaat taattagaaa
    19501 ctgtttcttc aagccatttt ctacattgcc caatatttac catagggtca aagccttggt
    19561 cttttccctt atttaaaagc atatcacaag ggaaagtatc acattcacca caatgattaa
    19621 gttccttact ttcacagcaa gttttaacct tacattctcc accccaaaat ggtttttcca
    19681 tagttgggca accagtgcaa tttacttgtt ctttttttgt acaactgtca caacagacac
    19741 cacatcttga ttcaaacatg ttcaaacatc ctttccatta tttaaaaata ttataaaatg
    19801 attttagatg actacaaaag tgcaaatagt tgattttaag tcatctaaat gttaaacata
    19861 aaattattaa tactggtaaa tttataaaaa ctattccaaa aatatttttg aatatttcaa
    19921 aacacaataa attacttcaa atccctttga atattccata acactataga ttactttaaa
    19981 tccccttgaa tatatttcac agcattatag attgctttaa atctctttga atatttcata
    20041 acactataga ttactttaaa tctctttgaa tattctacaa gactatagat tagtttaaat
    20101 tcctttgaat atttcataac atcataaatt agtttaaatc tctttgaata ttctacaaga
    20161 ctatagatta gtttaaatcc ctttgaatat ttcataacac tatagattac tttaaatttc
    20221 tttgaatatt ctacaagact atagattagt ttaaatccct ttgaatattt cataacacca
    20281 tagattactt taaatccctt tgaatattct ataacactat aaattacttt taacttttat
    20341 ataaccataa agtttttatc tggcacttta aatggagttt ttatatcaaa tttgccttcc
    20401 atcgaataac caaacttaat ttaattacac attccaagag tcaaatattc attcaatata
    20461 tttgataatt ctatgttata gccataatta aatatagatt attaaagtta aatatttatt
    20521 taatgtattg aatgattttt tagctatatt taattaagtt tcattatata atataaaaat
    20581 tgtttttgca ttaagtttta atataacaca taataatttt aaataataac attattatat
    20641 atttttttat tttaggagta tatctattat atagtgagta gataaaacat ataaaattca
    20701 aataaaagat atatattttt aaataaaagg gatataaatt tcaaaccaaa aagaatataa
    20761 atttcaaacc aaaaaatatc tagcatttac cattaagaaa taacatcatt ctttctaaaa
    20821 tttctattgg caatttcaga aaatactata gtagtaataa atgcaacaca tattgtaact
    20881 atagtgtcat taacatttaa agcagattgc tttataaccc ccatagcact acctaacata
    20941 gaataaggga aaaatatacc aacagcaggt tttgctacta ttaatcccat accaaataga
    21001 gaaaggcaaa gactaatacc aataggagtt gcaaaacttc taatctttat agacatgtat
    21061 aattgtatag aagctattga aatagaaata atccaacctt tagcaatcca accagtcaat
    21121 tctttaggta cactaccaga aaaaccaaac atatttcctg caactatata aaagataaca
    21181 aagaaaactt gtgtaatagc tgtaagtatt gaaactactg taagtttaga taaaaagata
    21241 ttttttaaat taactggcag tgtcattaca ttattccaat tatgattcat atgttcaagc
    21301 ctacaaatat atgcacaaaa gattgcgatt aagataggaa agaaaaattc cccataaaaa
    21361 agactaactt gagtccataa gctgtaccat tcttttttga gtatgccttg atttagataa
    21421 aaattaccag aaccaatcaa cacacttata agtggcaaag ctaataatac ataccatata
    21481 aaagaatttt ttaactttag aatttctccc aaaactgact tccttaacat ttaaatccct
    21541 cctatacttc tttttttaat aaatgatttc ttccaatata ataaattagc attccaatta
    21601 agaaaactaa aatcgtaata gaaaaattca tatcaattct atagacagat gaacctaaaa
    21661 cattatcata agcataactt aaaggtgata actctaggta ataagaccat ataaacaatc
    21721 tacgaatacc ttgataaaac aaacctgatg tcaaacctat aaaactacca atcatgccaa
    21781 atgtaattgc aatcatttga tttggaatta cagttgcaat ccataggtgt agtgcaatga
    21841 tagcaatact tacaagaatt gttcctaatg tgtattttaa aagtaaagat acagaaattg
    21901 actcaataac ccctctaaaa tgtccaaaca acacaataga taacacttgc atcaatatag
    21961 gtatagatac caaaatagca gaacataaaa atttggataa ataaatacta tttaaagatg
    22021 tcacagaaga ttttaaaagc ttccatgtat ctcctttatg ttctatatcg cttatgcgtg
    22081 atgtaattat tgatattaag ataggaaaga atagaccatt taaacttgca aaactcataa
    22141 gcatatagga atattcaaat ccacctaaca tatctggatt acgtgtaata gccctatttg
    22201 aatttacgat acaccacatt atttcaacag ctacaaataa aaaggtcatc agaataatct
    22261 tctttctttt aagtttataa aattctagac caaggtattt cattataaag tcaccgcctt
    22321 accagtaagt tctaggaata tatcttccaa gcttttttta tgttcttcta ttctaaatac
    22381 atctatattt gaatggacaa gcataccatt tattttagcc acatctgagt cttttaaatt
    22441 ttcaatagtc aagtacccat tttgtataag tgacatatat cctttagtat ttaatagctt
    22501 ttgtgcagat acaacattct tagctttgat agcaatctga ctttgacctc tattatgaag
    22561 ttcttttatg ctatcttgaa aaaccagttc gccatgattt attattccta ttgatgtggc
    22621 aatttggtca atttcactta aaagatggct agagattaga acagtcatgc cgtatttttc
    22681 tggtagagat tttattaatt ctctaatttc ctcaatccct gctgggtcga gtccatttgt
    22741 aggttcatca agtattaata atttgggaaa cttcattatt gccatagcaa tgccaagacg
    22801 ttgcttcata cctaaagaat attgattaac ttttttattt ttttgatttt ctaaccttac
    22861 aatttttaat gcttcattta catttttttt aggtaaatct aagagtcttt gcattatggt
    22921 catgttttca agccctgtaa gatgaccata gtatgatgga gattctatca aagaacctat
    22981 atcttttaaa attgatattc tatttttttc atttaactct ttacctaaaa tattaatatt
    23041 tccttgtgtg ggtttagcaa gtccaagaag cattttaaga gtggtagatt ttccagctcc
    23101 attaggaccc aaaaaaccat atacagctcc ttgtggaact cttaggtcta tatcctttac
    23161 tctaaatatt ttattgtatt cctttgaaag tctttgtgtt ctaattatca tttccatatg
    23221 tttcatcccc ttttttaatc tgtgcttatt gattctttta acttatatca gtataataac
    23281 ttggttacat ttcagttagc tgaagtttac cttacaataa ccttaaaagt ctatttaggc
    23341 aaagttgtga aaatgggtat atggtatact aaaggagggt gatgattatg gagtcaataa
    23401 aagataaaaa aatacttgtg gttgatgacc acaaggaatt attaaaaatg atagatgaaa
    23461 tcctaagaaa agagggattt agtagagtat ttttagcatc aagttatgaa gaagctgtta
    23521 gggtatttag aaatgtaaag ccagattgtg caattttaga tgttgtattg ccagatggag
    23581 atggtttctc tattatgaga aaaattagag agacatctaa gataccagtt atttttctct
    23641 ctgcaagagg tgaagatgaa gatagattga ttggtttggg attaggggct gacgattata
    23701 tagtaaagcc atttttacca aaggaactta ctttaaggct tgtgggtata ttaaatcgtg
    23761 tatatgtgcc tatagaagaa gaggagttgc cagtatttaa actgggagat tctgttgttg
    23821 tgaatttaaa tagtgcatgt gttgagaaag atgggcaaga gatttcttta acagctaaag
    23881 agcattcgtt gcttttaaag ttgtatgaaa ataaagggag aattgttact aatgattcac
    23941 tatgtcaagc catttgggga gatgatatgt atggatatga aaataccctt atggttcatg
    24001 ttagaagggt tagagagaag atagaaaaaa ctccatctac acctaaacat cttattacag
    24061 taagaggtct tggatataag ttgatgatta attaatttta gatattagaa taaaagaaat
    24121 caagaggagt gatttaattt tggatagtgt aaagtacata ttgaggcgat ttataggttc
    24181 aagtatcttg atttcattac tttttattgt attaaatata gcaggttttc ttctgttttc
    24241 atatatgcta tttgtatcta aatactctga taatttagat acaagtgata gcagagaaat
    24301 aaaatcaaac ttgattatga ttgaagataa tttgatttta aaagatggaa agtttatttt
    24361 agatgattct agtaaggaag tactacataa aaataaagtt tgggcaatgt taattgataa
    24421 caatggagat agaatatggg gatttgattt accaaaagaa attccaagtc attatacttt
    24481 gacagatgta gcaaagtttt caaggttctt tttaaaggac tatcctgtgt atgtatggga
    24541 acatccaaat ggaatattag taacaggtta tccaaaggac aaatacacta aatttaacct
    24601 tacttatacg attaaggaaa tagaacaatt tcctatgtta ataataggta tgtttctggt
    24661 taatgcaata gttacttttt taatagctct attcataggt ttaaaaatgg tgaaatctat
    24721 aaaaccaatt attactggca taaagcttat gtcaaaagga gaaccagtac ttttacaaga
    24781 aaaaggaata ctaagtgata tttcaaaatc tattaatact gtatctaaag agttgcaaat
    24841 gaaagatgaa aaacttagga agaaagagga agctaggtct aactggattg caggaatatc
    24901 tcatgatata agaactccac tttcaatgat aattggatat gctggagaac ttgaagaaag
    24961 cagtagcctt tcaaatagag aacaagagca agtgtctatt atatgcaatc aaggggttaa
    25021 aatcagagaa ttagtaaatg acctaaattt agtatctaaa cttgaatata atatgcaagc
    25081 attgaattgt gataagatta gagtttctgc gtttttgaga gagcttattt ctgagtttat
    25141 aaataataat ttggataatg acttttatgt tgaacttgat atactaaatg aagatattat
    25201 tatagatgca gataaaaaat tattaaaaag ggctattagt aatttaatac aaaatagtat
    25261 aactcataac caacaaggat gtaatataag ggttacctct aagtctgatt tagatttttg
    25321 ctatataatt gttgaagatg atggaaaagg tatgagtcaa gaaagaatta atagtttatt
    25381 aaagagccaa aatggactaa gtgaggcata tgccaaaggt caaaatcatg gtctaggtat
    25441 tttgatagta gctggaatta ttaatgcaca tagaggtaaa ttagatatta tgagtattga
    25501 tagtcaggga ctaaaaacaa tactaaaatt acctctaatt aagtgatatt ttataaaata
    25561 ataacagata agaatgaaga atgaagaatg aaaatatttt gaaaataatt ttattcttta
    25621 ttgacaaaat aactctatta gagtagaata aggataaatt cttttcttgt caagttttat
    25681 taaagtaaag agtaacttgt gctttatgcg cttatggctg agttactcat ggtatcaaaa
    25741 ttttcataga aataatattt tggaattatt gtaaggttat taaattcttt agataattcc
    25801 atttttttta ttttttagtt aatatttaat tttattattg acaaaaaaat tctactaaaa
    25861 tagaattatg gtatagtgtt ttttaatttt aagaaaaaat ataatgacat cctaaaaaag
    25921 ttttaattat acgttgttct gtgtagttat aatagcttct atgtaactat tgtaagtata
    25981 ttttattata attatttatt taaagtacga taaatgtttt aaattgaagt attttgcacc
    26041 attgagttct actaaagtag agtttttgtg tagtat
//
LOCUS       DAELXX010000055        25465 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.055, whole genome shotgun sequence.
ACCESSION   DAELXX010000055 DAELXX010000000
VERSION     DAELXX010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25465
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.055"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            127..1092
                     /locus_tag="KPH29_002086"
     CDS             127..1092
                     /locus_tag="KPH29_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBG1467677.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWNKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(1290..3401)
                     /locus_tag="KPH29_002087"
     CDS             complement(1290..3401)
                     /locus_tag="KPH29_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HBG1467678.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDNWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTAL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(3703..4410)
                     /locus_tag="KPH29_002088"
     CDS             complement(3703..4410)
                     /locus_tag="KPH29_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG1467679.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYRWGATGPNAFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYARRLITATRVLQ"
     gene            complement(4609..6522)
                     /locus_tag="KPH29_002089"
     CDS             complement(4609..6522)
                     /locus_tag="KPH29_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBG1467680.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISVCIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFVATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNSELKKVAVAIIDDDKDKIGRIIHNVKIVGTTSDIKAVVEKYN
                     VDEIIFSIANIEKRRKKEIIDICKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            complement(6606..7697)
                     /locus_tag="KPH29_002090"
     CDS             complement(6606..7697)
                     /locus_tag="KPH29_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG1467681.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            complement(7703..9046)
                     /locus_tag="KPH29_002091"
     CDS             complement(7703..9046)
                     /locus_tag="KPH29_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HBG1467682.1"
                     /translation="MPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLAEVGNENVV
                     CVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDVDVILIAVG
                     TPERSDGSANLDYIKAVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYINRNLKNNVK
                     VELASNPEFLSQGTAVKDTLYASRIVIGVESEWAREILEEIYKPFNQPIIVTNRNSAE
                     MIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLKAGIGYGGS
                     CFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQDMKVAVLGL
                     TFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYADSVDDAIE
                     DAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKIKDMEELGIEYYSIGR"
     gene            complement(9024..9776)
                     /locus_tag="KPH29_002092"
     CDS             complement(9024..9776)
                     /locus_tag="KPH29_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG1467683.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            complement(9824..10732)
                     /locus_tag="KPH29_002093"
     CDS             complement(9824..10732)
                     /locus_tag="KPH29_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG1467684.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            complement(10754..11503)
                     /locus_tag="KPH29_002094"
     CDS             complement(10754..11503)
                     /locus_tag="KPH29_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG1467685.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYKRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            complement(11532..12707)
                     /locus_tag="KPH29_002095"
     CDS             complement(11532..12707)
                     /locus_tag="KPH29_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HBG1467686.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            complement(12722..13561)
                     /locus_tag="KPH29_002096"
     CDS             complement(12722..13561)
                     /locus_tag="KPH29_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG1467687.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNEDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYTFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            complement(13564..14721)
                     /locus_tag="KPH29_002097"
     CDS             complement(13564..14721)
                     /locus_tag="KPH29_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HBG1467688.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNIDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRIILFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPIILTLFSVIIGTIFDKSTLLNRLLASRPKFWHVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            complement(14737..15459)
                     /locus_tag="KPH29_002098"
     CDS             complement(14737..15459)
                     /locus_tag="KPH29_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG1467689.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LICGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            complement(15471..16544)
                     /locus_tag="KPH29_002099"
     CDS             complement(15471..16544)
                     /locus_tag="KPH29_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HBG1467690.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEYISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            complement(16566..18272)
                     /locus_tag="KPH29_002100"
     CDS             complement(16566..18272)
                     /locus_tag="KPH29_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBG1467691.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNDTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            complement(18314..19873)
                     /gene="murJ"
                     /locus_tag="KPH29_002101"
     CDS             complement(18314..19873)
                     /gene="murJ"
                     /locus_tag="KPH29_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBG1467692.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDDKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRRR"
     gene            complement(19988..21043)
                     /locus_tag="KPH29_002102"
     CDS             complement(19988..21043)
                     /locus_tag="KPH29_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HBG1467693.1"
                     /translation="MIKKISTILSLVLLISISSTIEVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGIKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTAKLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            complement(21138..21824)
                     /locus_tag="KPH29_002103"
     CDS             complement(21138..21824)
                     /locus_tag="KPH29_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBG1467694.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            complement(22044..24068)
                     /locus_tag="KPH29_002104"
     CDS             complement(22044..24068)
                     /locus_tag="KPH29_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG1467695.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQG
                     TYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHSTNLSEKTAGT
                     VYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGAGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            complement(24322..25380)
                     /locus_tag="KPH29_002105"
     CDS             complement(24322..25380)
                     /locus_tag="KPH29_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HBG1467696.1"
                     /translation="MIVCNNPGTWMRMYPQLRHAVWHGVTLADFAFPFFVISLGVTIP
                     ISINSKLKNNKSTLSIILSIFKRSILLILFGFFLNYLGNPDLNSVRILGVLQRMGLVY
                     FVTSLVYLLLKKLNVGSTATIITFLCIATFIIVGYYILAKPYGFELEGSLAQLVDLHF
                     FKGHLYKPEFEPDGFLTSIVAISSGMLGCTMGCVLLKENIGEYKKFFKILVMSIILLI
                     GAFIFNQYFPFNKRLWSSSFVLLMAGSYGMLLSIFYFICDIKNKSKIFTPIIALGSSP
                     IFTYMCLEILSHVFWNVPKLTNKVDYPTTLVEWTTYELITPWAGTTWDSLIFSLLYVL
                     FWVIVMSIMYKKKIFIKI"
BASE COUNT         7942 a         4633 c         2764 g        10126 t
ORIGIN      
        1 ttataaatta tatcattgtc ttttttttgt aactgaaaca agtattgaat atatctcaat
       61 actttaatat aattaagtgt atgctattaa aaaatataca ggaaaaagaa gaaagaaggg
      121 gtacttttgt caaaattaaa gaaatttgtt atactactgg ctttcttggt agtaatattc
      181 cctataagtg tctatggata tttttattat aaattatctg ctattcatga ctcgagcata
      241 agtagtgatt tattggataa caatgaccat aaaaatgaag atgggataat taacatacta
      301 cttatgggaa cagatgctag accaaatgaa gattcttcaa ggtcagatgc tatgatgata
      361 cttactattg ataacaaaca caatgatatc aaacttacat cattagctcg tgatagctat
      421 gtagacatcc ctggtcatgg taaacaaaaa cttactcatg cttatgctta tggacaagct
      481 gatttattaa tacaaacaat tgaagagaat tttaatattg atattcaaaa ttatgcttgt
      541 gttaatttcg aatcatttat gtatataatt gatgctattg gtggcgttga ggttactgtt
      601 gaaaaaggag aaatacgaga actaaataaa tttattcctg aaacttataa atggaataaa
      661 agtgatgata aaggtagcat acaatacatt agaaactctg gaaagcagac tctaaatgga
      721 tatcaggcac tatcttttgc acgtatcaga cacaacgata cagcctttgc tagagatggc
      781 agacagagac agattataca agctataatc aagaaaacgg aaactttacc tgttactaaa
      841 tatccaggtt tattagatgc agttcttcca tatgttaaaa ctaacatgaa gcctaatgca
      901 atactttctt taggagctca agttttaaaa atgggtgact taaatattaa gcaatttgaa
      961 tttcctattg atgatgaaat tcatagtaca ggtggtatat atggaaaagc tggatgggtt
     1021 cttagatttg acccagatac tttagacata ttacacgatt ttatatttaa tgacatagag
     1081 tttaaacaat aaaaaattca acataaagtt tattaaaaag tataagatta attactaaaa
     1141 aagaactata tcgcataatt attttcaagt tgctgtgtac acttgattac aaaatgcata
     1201 tagttctttt ttattttcta atttatgatg ataattttca ttttaatcta catttataaa
     1261 atactctgta ttttaaatta acttttttat tacttaacta agtttaagaa agtgtttaat
     1321 gtgttttgat ttataccatc tccagcttcg tatactagag cagttttctt aggctctaaa
     1381 gcagttttct gagcagatgt tacactattt ccaagcataa ctactggtga ttttaattta
     1441 gctgctaaag gacctgatgt taatgcatct accaattgat tacttttact tacaaacatt
     1501 gatgaatatt cagcatttgg atagaatttt tgtattactt ttccatttgt ttcttctcta
     1561 ttttctccag ctattctatt tttagataca tcatttgtaa tgaccttatc cagcttattt
     1621 attactgaat cagatatact tagattacca cctataaaat aagcatcttt tacttttaaa
     1681 tctgatatcc aattgtaaac ttcatcactt acattatctt ttgatactag tactattggt
     1741 tgcttttctt ctcctgcttt tgaagctata gaaagagaat ctgcttcacc tgtaccactt
     1801 gttatatata gtttttctac tggattagtt tttactaatt cttttgctat cattaaagat
     1861 gtactatatc tatctgaacc accaactcta tttattgata catttgacaa tgtatctttt
     1921 atctcactct caactttatc tccaatagag ttatttcctc caattaaaat aactttactt
     1981 ggattaagtc tttttaactc attcttagtg gatgtaggta tattatcctt atttaccaat
     2041 agtataggag catcatttga tgtagcaagt ggtgttgatg ttatgccatc aactatagag
     2101 tacccattta ctaatactac tgtatctgaa tttgtagccc aacctttttt acttattgct
     2161 actgctgtgt catatctttc gtcgcctctt aatttctcca ctttaacatt agatggtgga
     2221 gtaactggtt cttcactact agaatataaa ctctttaaat ccttttgtat agaagtacta
     2281 tttgctatat ctttagctga gaaatacata ctacctttta tttcactata ctttctattt
     2341 aatgttactt gttgtactat ttcttttgct atattttgtc caccataact agattgacct
     2401 tgcttatata taccttgtcc tatgtataag tctacatttg ttccttttac ttcattagcc
     2461 caccatgcca ctaatttaga ataatctgct gctttaagac caattggcca gtaaagttgt
     2521 ggaactacat agtcaatcaa tccttgtttt atccatgctc ttgtatctgc atatgtacct
     2581 acataacttt cattacctga tgtatctgaa cctgttgggt cactactctt atttctccat
     2641 atacctgctg ggcttacacc aaatacaaca ttaggcttta tactctttat agacgctttt
     2701 acatctctaa gtagagtatt tacattttct cttctccaat tatccttatt ttgtcctttt
     2761 ccatattttt ggtatgttgc agtatcattg aaacttactc ctggatagaa gtaatcatca
     2821 aaatgaactc catctatatc gtaattttga actacttctg ctatactatc cactatgtac
     2881 tttctaactt ctggtaaacc tgggtcgtag taatacttat ttccatgttt aactacccag
     2941 cttggatttt tctttgctgg atggtttgct ggcaatttat ctatgctctc atctgccata
     3001 gttattctat atggattaaa ccatgcatga aattccattc ctcttttatg tgcttcttct
     3061 attaaaaatg gtaatgggtc atatccaggg tctttacctt gtgtaccggt aagatattca
     3121 gaccatgggt ttatatttga tttatataaa gcatctgatt ttggtcttac ttgaaccact
     3181 gctgtattta ttcctacact ttttagtttg tccaataaat ctgtgtattc ctttttttgc
     3241 ttggcttcat tattcttagt cttaggccag tctaaattat aaactgtaga tatccaagca
     3301 gctctcattt ccttatcatt tgatgaatcg gcaaatgaac ttacactaca catagtaagt
     3361 gtcattatca atgataatac taatatactt atttttttca tttcatcccc cttaattttt
     3421 taaatttatt ttttctacaa tatacttctt attaagtata tctaaaattc atattatctg
     3481 tcaaatattg aataatatag ttaaaattta tatttgatat ttgattataa tatgaaataa
     3541 tttattataa aaaaaacagc aaagaaattt atcttctttg ctgtttttat aacgttcatc
     3601 actattttaa tattactaaa ctcttaaatc tctatgtaaa acattaaatg taattatata
     3661 tttaatgtta atattcaatc ctatgtaaat caatatctat tattattgta aaacacgtgt
     3721 agcagttata agtcttcttg cataattagt tcctggagcc atgctagtaa cttttactac
     3781 atctcctgtt tgtggtgaat gtatgaattt agaatctcct acatacaatc caacatgatt
     3841 tattgaacca cctgaaccaa agaaaactaa atctcctggt tttaattctg ctcttgatac
     3901 tttctttcct actgttgctt gtgatcttga tactcttggt aaattaaccc ctgctccatt
     3961 tttatagaca taagaagtga acccagaaca atcaaaagca tttggtccag ttgctcccca
     4021 tctatatggc ttaccttgtt gcttaaatgc taagtttaaa acattttgta cctttgcatc
     4081 tacatttcca ccttgaatag ttaaataatc tccatttacc catcctgtaa cattgtcaac
     4141 actttttatt ttaatccatc catttgaatg taattctaaa acttcaactt tgtctcctgt
     4201 gtttatcttt cctactactg catcatttgc actagctcca cttcttacat ttaaaaaatc
     4261 acactggtct actgttgcac tcataggtga attagttggc tctgctgcac tagccgttat
     4321 tgctcctgct tctactgtga caactcctgc tattaacacc atagctataa attttttcaa
     4381 tatatgtttt ttatttttaa tattcatcat aatctcaaat acctaatcct ttcgaaaatt
     4441 taacattttg ttccttcgtt tccaattcct aattaacatt ctatcttaca tcaaaataaa
     4501 ttgcaacctt ttttgtaatc tttttgtaat ctttttgtaa tcttttgttt ctttttagta
     4561 tatatttgtt tctttttgct ccccctggtt acaaattaga aatatattct acttttctct
     4621 agttgttgca atttctttga tattagcatc ttctggttta atataagttg gcactaattc
     4681 cctcataata ccatctattt tttcaacctc ttcattgtta gtaatttctc ttaaaagttt
     4741 taagtatttt gttatttttt ctctatcaac atctattggt ctaccaataa aaatcttttt
     4801 atgctctgta tccaacagac cttcttctga cataagtagt tcttcataca acttttctcc
     4861 tggtctaaga ccagaaaatt caatttttat atcaacatct ggctcaaatc cactgaattt
     4921 aattaagttt tttgctaaat ccaatattct tacaggttct cccatatcaa gcacaaatat
     4981 ttctccacct ttagccatag ccccagcttg tataactaag cctactgctt ctggtattgt
     5041 cataaaatat ctaatgatat ctggatgagt aactgtcact ggtccgccat cttcaatttg
     5101 cttcttaaaa agtggaatta cactaccatt actaccaagc acatttccaa atctaacagc
     5161 aacaaattca gtctggcttt cagcattcat agtctgtata atcatttcag cagctctttt
     5221 agtagctccc attatgttag tagggtttac agccttatct gtagatataa ggacaaattt
     5281 cttagctcta tattttgaag acaagccagc tatgtttaaa gttccaaata tattattctt
     5341 tatagcttcg ccagggctcg attccataag aggaacatgt ttatgtgctg ctgcatgaaa
     5401 taccacttct ggtttatatt tattaaatat ttcatccatt cttttttctt ctcttattga
     5461 tgctattact gtctttaaat ctaatttatt tttatatttt ctaagtaatt cttgttgtat
     5521 tgagtaagca ttattttcat aattatcaac tataataagc tctttaggat taaatcctgc
     5581 aatctgtctg caaagctctg aaccaattga gcctcctcct ccagttatta ggattacttt
     5641 tccctcaatg taattactta tctctcttaa atttgttttt ataggctctc tacccaacaa
     5701 gtcttctatc tctacttctc taatctgttt tatatcaact tttccatcta taatttcgta
     5761 aattcctggt attgtcttta ttttacagtt agtgttttta catatatcaa taatctcttt
     5821 ttttctacgt ttctctatgt ttgctattga aaagattatc tcatctacat tgtacttttc
     5881 taccacagct tttatatccg aagttgttcc tacaattttc acattatgga ttattcttcc
     5941 aattttatct ttatcatcat caattatagc cactgctact tttttaagtt ctgaatttct
     6001 ttttagttcc tgtataacca tttctcctgc atctcctgca ccaattataa ggatatttga
     6061 aacctttcct ctagaatatg tcttgttcat gactattctt atagctctat aagctattct
     6121 tgcgccactt gtaaacatta taataaatat tgtatttaat acataaaaca ttataggata
     6181 atcaaaccct aataatttat ttattatata aatcggaatt aaataagcta aagttgccac
     6241 aaaaatagat ataatttctt cttctcctgc atatctccat aatgtgttgt agcacttaaa
     6301 caatctaaat gatactatat gaagtactat atatatacac actgaaatta ttatatcatt
     6361 agtggtaaat gcatttaaat tacctgtcaa gcttaaagac attcgaaacg ctactagtac
     6421 gctacaaata tctagaatta atagtgttaa atgtctaaat ttatattttt taaaaagcct
     6481 attaaaaaag ctttctttct ctaaatctat actttctcgc ataactagaa gccctccttt
     6541 aaacagcaaa agctacgcac ctaagcatag ccaacaacat ctatcttttg ctgtattttt
     6601 tcattttatt ttttatattt atcaaatata gaatcataaa ttttttctat atcattaaag
     6661 tttaaattaa cctcataatt ttctctaaca aattgacgcc ctttcttacc catattaagt
     6721 ctaagttcat cattttccat tagttttatt atagcatttt ttatttcttc tggttgtttt
     6781 ggttctacaa ttaagccagt ttcattttcc catacagatt ctggatgtcc tcctacattt
     6841 gtaactataa caggtacttc acatgcttct gattctatag ctgccactcc aaatccctct
     6901 cttaaagaag gaaatacagt tacatcaaaa gaattaaaag tcttgcttac attttctgga
     6961 gatattcttc ctaaaaactg aacatcattt tctataccca attctttagc taaatttatt
     7021 aggttgtcca tctgacttcc agagccacct attttaagaa ttattttctt atctttatat
     7081 tcatctttaa gcatcttaaa tgcttgtatt aaatattcta ttccatattt tttttctaaa
     7141 ctctttataa ttcctattac aaaagcgtct tctttttcta cttccatagg cttaaaccta
     7201 tccatatcta ctccaaaaaa ggttacatct acatgtttag ctgtatattt attagcctct
     7261 ttagccatat ctctactatt tgaaaagata tagtctgctt ttctaaagtt atgttttata
     7321 atttgttttt gtataaaacc attattaggg aaatcataaa tatcagtacc ccaaacagaa
     7381 ataatatatg gatgataccc aagtagactt cctataaatc cataactact tgcatactgt
     7441 gcatgcaata tatctggctt tatttcattt actagcttct ttatctgctt tattgaacct
     7501 aagtatcccg tttttttaga aatattttca ttttttaatt cttctacatt agaaccaaaa
     7561 ttataaattt ttattccttc catgtgacct ccatttaaag agattacatg aatgtcatat
     7621 cctttatttt taaaaaaact acaccatttt aaagtatgag gatttgaccc atcagctaaa
     7681 taacaaacag tcttcataat atttaccttc ctatcgaata atactcaatt cctaactctt
     7741 ccatatcttt aattttataa caatttcttc catcaaggac taaaggattt ttcatctttt
     7801 ccttatactt atgtatatca taatttaata catctttcca ttctgtcatt ataaaacata
     7861 tctctgcatc ttctatcgca tcatctacag aatcagcata aagtatagag tcaccaaata
     7921 ttttcttagt attatttaca ccaattgggt cgtatacagt aactcttgcc cctctttcaa
     7981 gcaaatattt tatatttggt atagatggtg cttctcttat atcatcagtg tctggtttaa
     8041 aagtcaaacc taaaacagca actttcatat cttgaaagct ctctactaac ttagatgcct
     8101 ttctaactaa tttaagtttt tgactttcat taacttctat tgcagctttt actgttttta
     8161 attcataacc attatgttca gataaccagt gaagagcttt tgtgtctttt ggaaagcaag
     8221 aaccgccata ccctattccc gcctttaaaa acttacttcc tattctatca tcataactca
     8281 ttcctaaagc tacatcttct atgttagctc ctgctatttc acaaagattt gcaatatcat
     8341 tcataaatga tattttaaga gctaagaagt tatttgatgc gtattttatc atctcagcac
     8401 tatttctatt tgtaactatt ataggttgat taaaaggctt atagatttct tctaatattt
     8461 ctctagccca ttcactttct acacctataa cgattcttga agcatataga gtatctttta
     8521 ctgctgttcc ttgtgataaa aactctggat tagatgcaag ctctactttt acattatttt
     8581 ttaggtttct atttatataa tcttctatct cttcatttgt accaattgga actgtagatt
     8641 ttattactac tagagtatct tttgtcacag tttcagctat ttgctttgca actgccttta
     8701 tataatctag gttggctgag ccatcacttc tctcaggtgt acctacagct attaatatta
     8761 catctacatc ttcataagct tttttatagt ctgtagtgta atctattctt cctctgttgt
     8821 aatttttaac cataagttct tctaggcctt cttcatatat aggtgctatt ccaagtttca
     8881 ttgatttaac tttttgttca tctacatcaa cacagactac attttcattg cctacctctg
     8941 ctagacaaac acctgtaacc aaacctacat atcccgtccc agctatagct attttcattt
     9001 ttttacctcc aagtatataa aacttatact ctttttttaa tggcattaaa aacatagaaa
     9061 caaaatacat atagactttt tggtaaactt aaattttcaa catttctata agtattccaa
     9121 gttcgtttta atgcttttat cttgttactt gatatagaat ttccaactaa tctgtaattc
     9181 actaaatcct cctgaatccc atatgcatat ttttcctttt taagtaactg taaccaggtc
     9241 gcagtatctt gtcctcttct taccaaaggc atgctgaact ctcctacaat ctccttatca
     9301 atgaccactg tagaacatcc tattatagta tttctaagta atccttcata gtcaattttc
     9361 ttaggagctc ttgcaacctt attcgttttt gacccatctt gtctcatata tctataagat
     9421 gtaaaagaaa aacctacatt ttcttttaac atatattcta tctgaatctc taattttcta
     9481 ctatcccata aatcatcact atctacaaat gcaataaatc gaccttccgc attcttaata
     9541 ccaacatttc tagatactgc agctccagaa tttttttcaa gctttatata ttttattcta
     9601 gcatcttctt ttcgaaaact ttttataatt tcacttgaat tgtccttaga acaatcatcc
     9661 actaataaaa gctgccaatt tttataagtt tgattttgga ttgactttat cgtctctgat
     9721 aaaaactctt ctgaattata aacaggagta attatcgata ctaaaggctc attcatattt
     9781 ttactctcct tatatagcaa ctagcagtac tataccaaca agtttactta gatactaatt
     9841 ttctttgtcc aatatatttg taatatccta atgggaataa tgctaaatac aacaatttat
     9901 cagatacttt tcttaattca ccaaaataat gactatcaga caacaatgag tatctggcta
     9961 aatttatgta attttttatc cacatatatg gatatctact taatggatat gtattttgat
    10021 ttattaaata taaaaactct aatgctctac ctcttaatgc atttttactt aatatattct
    10081 tagtcaaacc atcatccaaa taatctctta ttatcatagg tttatttaca catataactt
    10141 tatagtattt agacatttca ttccatacta cactttctgg taaaaatctc acttcttttc
    10201 tttctggaaa aggaaatttt ataagctcat cagttacaaa tactatgctt ttatctccct
    10261 taacgccata cttaaaatat aggtctgcaa agtgacatat tttcttatct tcaggcattt
    10321 ctgtccctat caaatctcca cttggatata cacaaagtcc aacaactcca ctaaatcctt
    10381 ctttattctc taaagaatcc cactcatcat tagcaatttc aagagaatct ttaattaaac
    10441 catcatcact atctacaatg aaaaatagct ctccatctgc attttttaca cctagattta
    10501 ttgcagtatg ttttcctcca ttttctttgt atatatatct tatatttaat ttattttctg
    10561 atataaattt ttcaactaac tctttagtac tgtcgctact tccatcatca acaatcaacc
    10621 attcaaaatc attgaaatca taagtttgag cttttaaatc attgtacaaa ttttcaagta
    10681 aatgagctct gttatacgtc ggagtaaata cagtaaattt atatttatac attaattaaa
    10741 tcttcctctc tgtctaactt tttttgattc cgtttattcc ataattaata aagtagtata
    10801 tagctttgta taaaggtatt ttttctatat ttctatatat attccaagtc cacatagcag
    10861 cttttatttt attaccactt agggaattgc tagattttct atataatgct agaacttcat
    10921 ttattccatg agcaatatgc ccttttttca atatcgataa ccataaaaca tagtcttcat
    10981 gtcttacacc actcattctt atttcaaaat ccaatttaga cttatcaatc acaacagtaa
    11041 gacatcctaa gatatttcct tttaataatc ttttataatc tacattaggt ggaactttta
    11101 ttactttgtt taatttttta tcattttcat ccatcagttc atatgaagta aatgaaatta
    11161 catagtcatt ttctaacata aaatttactt gtttttctaa cttactgcta ttccattggt
    11221 catcactatc taaaaaagct ataaattgtc ctgttgcctt acttagtgct aaatttctag
    11281 cattagagac acccttatta gtctcagtct ttatacattt tattctacta tcctgttgca
    11341 tataagattt gactatatta ggactattat ctgttgagca atcatcaata attaacattt
    11401 cccattcttg ataagtttgg tttaatactg attttatggt tgcttcaata aatttttcag
    11461 aattatacat gggagtaatt atagatacta aattcttctt catattacct tcctcatcat
    11521 ttataaaata tttatttaat tttacttaat acacaatcat atatcctctt gcagttctta
    11581 ttatcattat atctgaaaaa cttatttctt tcattttttt ggttatccat aacctcaaaa
    11641 ttattttcaa taatatcaat aagtttattc acggcttcat cattattata agccaaagac
    11701 ccaaacattt cactttttaa atctatatat gaatcaactt tttccatata ttcatccaaa
    11761 tcgaattggt aaaacaagat aggtctattc atatacaaaa agtctatggc tacacttgaa
    11821 taatcagtta tatttgcagc tgattttctg atttcatttg ctagagttac ccctttatct
    11881 aaaaacacaa ttcgttttcc tgttatattt tcttttatat catctataaa ctcatgcata
    11941 agatgatgca tataaatttt tacatttata tctttctcat ctaatatttt atttaatttg
    12001 tcatcattta aaaaattcat tatattttta aaataatctg tatccgtaaa ttttcctgaa
    12061 ctaaatttta tccaatctct ccatgtagga gtatataata tatctcgagt ttgtgcaact
    12121 tcatttgttg gtaaattgtc atatcttgca ttcccaatta tataaactga gtcatctaac
    12181 attccccaat catttaccat aacatttctt tcaaattcag aacttgctgt aactatatta
    12241 tagcttttat ataggttatc tataatattt ttgaactttt tgttcatagg ttttcttttt
    12301 ttaaagctta tagtcccatg atttaaaaac acttttatcg tttttttatg aaaataatta
    12361 agaacaggta cagcaaaatt gtatggtgct atatctgctg atggtgcatg tgaaaatact
    12421 atagcttttg aattgtaata atacagatag ttttctacac ttcctcttat taattttttg
    12481 cctggaattt tatcaaatac tttactgtct ttatttacta cccaataagt ttctacatca
    12541 ttatgttctt ttagcatata ctcataaaaa aactttgaat tatcattgta tatatcccct
    12601 gaatgtccac ctataagcca gatatctcta ccattaaact tatgcttatt aaatggatat
    12661 ataaaaaaag ccaaaagcat atttattaaa aatttaacct tgtccataaa caccatcctc
    12721 attataactt gtactgtcta aatccattaa ttcttccttt tatagtggca ctatgataat
    12781 ctctattgga actcaatata agacccccta tatttcttac aagttttgat accataactt
    12841 ttgcaaacgt ataatttttt ctatagacta tttccttttt gcaaagcgct ccaaagcctc
    12901 ttccataatt ataatctttt tgatactttt ctttagattt agaatgtttt tttgcaggat
    12961 ggtaaattat atcatttcca aagtattttc ctttaaaacc taaactcaat aagtttaaaa
    13021 cataatctat ctcttcacca gcaccaaact ctccaccaac acccaatttt tcatcaaatc
    13081 ttgtataatc tttaccttca aaatcaataa aaaaagttat ggatgttata gtatccaaaa
    13141 cgttggaact tgtgatatca caagttccat catacatttt cttaaatgca tcaactttat
    13201 ttgaatccat agttttgcaa ctgtatattt tataatcttc atttttatta aagaaattta
    13261 taactttttc taatgtatca ttttcgtaga cacaatcatc atcagggaaa cctataattt
    13321 gccctacagc attatctatg cctacatttc tgttataact caatcctttt ttagagcttt
    13381 ttatatactt tatatctaac ttatcaatgt atttatcaac tatttcttta actttgctat
    13441 tatcattttg gtctactact ataagttcaa agtttttgta agtttgattt tctaagctat
    13501 ccataagtaa gtatatatca tcatatctat ttaatgttgg cattattaat gatattaaca
    13561 ttactacctc ctacttttca tcttccacaa atctatttcc cttttatcat ttaatatcac
    13621 ttcttttatt agtaatacta ctgcaaaact aaatgaagct tctaataata aattttctgc
    13681 aaaagagtat agtaaaaata taaataaagc tactaaatat gcttttttat ctttttctat
    13741 aaagctttct attcctttat acatcaaata cataaataat atacaagcaa ctactcctag
    13801 tacactaatt atatatacat aagaactatc taatggatta gttgctttta tagcaggact
    13861 atatgaattt ccaaaaggtt ttagaaaatt ccccttttca gccaagtata catgccagaa
    13921 cttaggtcta cttgctaata atctatttaa taaagtgctt ttatcaaata ttgtaccaat
    13981 aattacacta aatagagtaa gtattattgg taatgtcttt aggagcattg gcagtccttt
    14041 tatttttttt atatctatcc atcttattat ttctacaaat attaatcctg ctaatatagt
    14101 gaaaaatcca gttctactat atgtggtttg ataaacaaaa aatgcagaac caaataaaat
    14161 tattctatcc catttattat aatctttata tcttaagaat atatatgctg catatatagt
    14221 aaccatatga taaaatacac tatttggatt accaagtcca aaatccattc ttgtaacaag
    14281 catatctatt ttttctatat atctatagtg gacttcctga ttaaatatta atgaaggaaa
    14341 taaattaaat aataatatag ctaccattaa aaaacatgcc gatattatta aataattttt
    14401 aactataaat ttatcatcta tttgcttagc tgatacagct aataaaacga ttaataatat
    14461 agctccttcc ctatttaata aataaagtaa gacacaaact ccaactagta ccatttctgt
    14521 tatacttttt ctcttatcaa ataaaaaaag tagcattagt attcctattg aagaataaaa
    14581 taacgctgat ttaatcatcc ctatatctat atttgggtca ataaatgata tcatatactt
    14641 tctaagtaat atgataaaca aaacaaatat ataaagatat tttctaatca taatatttct
    14701 atcatcattt aaagtcttca ttttactttc tcccttttag cttcgtaaag tattacttgc
    14761 aaatatttta caacacttcc atatcttatt tacacctgtt aagtgtttgc tttctaagaa
    14821 ataatttctc tttttagccc aactttttcc ccaccaatgt atcccaaaag tgttttctgt
    14881 tatacattct ggtgtaaagt cttctttaaa tccaaaagga taaaaatatt ctttgtcgta
    14941 aacacgtatt ccttcttcat aattatttgg gtcaatcttc ttataattct tttccataag
    15001 atgagtcatt atttttggta ttgtaaacaa tgaagaagta agtacttctt tttcataata
    15061 ctccaataag tcttttaaaa aaggatgtcc tttttctaca cctattatag caccatttat
    15121 atactctcta tcatcttcat acccacagat tagcctatta ttttctaaaa gaggagttat
    15181 atcctttaat atttgcatat ctgtatccat ataaactcca ccttgttcat agagtacttt
    15241 tattcttgta taatcagata tgaatgccca aagttttctt ttataacatt cttccaaaaa
    15301 cttatttcct tttatttctt tctctatatc aaagtttttt tcattccact ctactatctc
    15361 atattctgga agcttttctt tccaagaatt tatacatata ttttctatat ttccttttgg
    15421 accaccaaac catacataat gtattttttt aggaatcaaa tgaacacctc ctacatatat
    15481 tcttgtctat cttcttcttt taactgttct actatttttt tgacatcttg agctttatct
    15541 tttctacaaa ccataactgc atcattcgta gatactacta tcaaatcact aatacctatt
    15601 gttgatatta atttgtcatc tgaatatata attgaatttt tagtatctat agtaatattt
    15661 tcacctcttt tgatgttacc ttcatcatca gttggatata ttgcacctag agaatcccaa
    15721 gaaccaacat cattccatcc aaaatcacca ggaactacta taacatcatt acttctttcc
    15781 ataataccat aatctataga gattgattgt atagttgggt atatttcttt tattttttcc
    15841 atttcttctt ttgttcctaa gtatttactt atatattcta atttttcata tacttttggc
    15901 aagtatctct taaaatcttc taatatttta gataccttcc atacgaacat tccactattc
    15961 catacatact ttttagagtt tacatattct ttagcaattt catagtttgg tttttctaca
    16021 aactccacta cctcatatgc aacatcttct atcgtatttt ctctattaaa atttatgtat
    16081 ccatatcctg ttgatggaaa tgtaggagtt atcccaatag ttactagttt atcattattt
    16141 tctgctatat agatagcttt ttccaaaata gatttaaatt ctttttcatc ttttatataa
    16201 tggtcagctg gatatacaca cataacacca tcaccatatt ttttcattat gttaaatgct
    16261 gcaaagccta tagctgctgc tgtatttctg gcacatggct ctggcaatat attactatcc
    16321 aaacatttat cttttactat atcctttaaa gcttccaatt gcttctcatt ggttactata
    16381 aataaatcat ctttttttgc tagagaatct attctgttta tagtttcgtt tattaaagca
    16441 tcttctccac ttaaatttat aagctgttta ggtacttctt gtctactaag tggccagaaa
    16501 cgagttcctc ctcctccagc cattatgaca ttgtatactt tcattattta tctcccctta
    16561 gctttttata tagaatccac tacatttaat atataagata ttaaattgtt caacttaaat
    16621 tctacatctt cttgattgtc tgaatttgct ccaaagtaaa atttaatttt aggttctgta
    16681 cctgaaggtc ttatagctat ccaagagcca tcttcaagta aaaatttaag tacatctgac
    16741 ttaggtaaat catcaacacc atctttatag tctaaaattt tatctacttt tacatcagcc
    16801 acattatcta ttttaacact tctaaagtaa agcatcatct cttttatttt ttttattcca
    16861 tctatacctt ttaaagttac tgactttaag tcctctttgt agtatccaaa tttattatat
    16921 gtatctatca aagcttcata caagttcata ccttttgaag aatagtatgc tgccatttca
    16981 catatcataa gtgaagatac aacaccatct ttatctctag catgagttcc aattaagtaa
    17041 ccatagcttt cctcatatcc cattataaaa cttctatcat tgctttcttc aaacgctttt
    17101 attttttctc ctataaattt aaatcctgtt aaagtattta aacaatctac attataagcc
    17161 tttgctattt ttgcacctag ttctgatgta actatagttt ttattattgt aggattgttt
    17221 ttaggaagtt tattttcttc aactaaactc tctaatatat atctaactaa aagtgaaccg
    17281 acttgatttc catttaagac tacatattca ccacttgaat cttttacaac gatacctact
    17341 ctatcacaat cagggtctgt tcctattatc aaatcagtgt cattattctt tgccatttca
    17401 atagctatat taaacactga cttttcttca ggattaggat actttactgt tgaaaaatta
    17461 gaatctggta attcttgctc cttaactacc attacatttt caaaaccaca ttcatttaag
    17521 acttttctta ctggtttatt tcctgtacca tgaataggtg tatatattat ttttaatttt
    17581 ttcccgtatt catctattat attttgtctt aaaacttgct tcttaacagc ttttataaac
    17641 tcatcatcta cttcattatc taatatagtt attaaatttt tagataaagc ttcttcaaaa
    17701 tctatacttt taatagtgct gtaatcatca atcttattaa cttctgctat tatatcattt
    17761 gccatatcta tacagatttg accaccatct gagtcatata ctttatatcc attgtattct
    17821 ttaggattat gacttgctgt tattactatt cccatagcac actttaaata tctaacagca
    17881 aaagaaagtt ctggtgtagg ctttaattct tcaaaaatat aagcctttac accataagca
    17941 cttaaagtct ttgctacttc tatacaaaac tccctagaca tgtatctact atcatgtgct
    18001 ataactattc ccttttgttt accttcttct ccttgagtct tcatgatata atttaaaacg
    18061 cccagtgtcg cacgtctaac agtataaata ttgattctat tagttcccgc tcctattata
    18121 cctcttaaac cacctgttcc aaactctaaa ttcttataga atctatcttg tatttctttt
    18181 tcatcatctt ttatgcttaa tagctcattt tttgtttgct catcaaaata aggagaattt
    18241 aaccacatct cataattatt cttataatcc atattttcat cctttctgac tctaatttaa
    18301 aatttcaagg taattatctt cttctaagta gtttttgttt tccttttctt aaaaattcaa
    18361 aagctaagtc catctcttct accttaaata aaactatcaa tacagtatat acagaagctc
    18421 cacctaatac tccagctcca actgatataa tttctttaat tgttcctgaa cccataaatg
    18481 catatagatt attatatatc aataaagtag ctactgccat cacaccagat gctaccaaac
    18541 tttttagacc agtctttata attttatccc ctccaaaata tccatttttc tttttaagag
    18601 atataaataa caatataact gtaattatat tggatgtact agttgatata gcaatacctg
    18661 catgacctaa tggtcttatc aatattaaat taagaactat atttattatt aaagctatac
    18721 ttccatttat cattggcgtt tttgtatctg aaagagagta aaatgcccta tataagacat
    18781 ctcttactcc acaagccaat aaacctagtg aatataatct aagcgctata gatgtaagat
    18841 ctgttgataa cgcatcaaat ttaccccttt gaaataatat cctaacaaga ggctctgcta
    18901 atataatagc tcctatagat ataggaagca ccaccaatat aatacaattt gaagatttta
    18961 caatagtact tataaatcct tctttatcat ctttaccaga catttttgct agcttaggat
    19021 atataactgt tattatagat gtaacaaata atgccataac aaactcattt agcctatttg
    19081 catagttaag agcagacaac tttccatcac ccaaagtagt tgccaataat ctgtcaacaa
    19141 atatatttaa ctgtccaaca gctactccta ccaacatagg caatactagg tttactaatt
    19201 cttttataga ctcatccttt aagtttatat atggcttata cttgtaggac ttcttaaatg
    19261 caaatggcag ttgaaataat agttgactcg ccatagccaa tagtgctcct gctggtagta
    19321 tccatacgtt tttacctgca cttagtgcta ttgctgctat tattataata ttatatggga
    19381 ttcctataag cccaggaatt acaaaactgt cattcacctg taaaaacgaa ctaaatatct
    19441 tacttccact taggaataaa actcctgaaa tcattatttt agtaaataat attgctgctt
    19501 ggaacttagc agggtcgttt ctaaatcctg ctgcaaatac tgtaacaaat tgctctgtaa
    19561 ataaaagccc aataatagct attactatag ccattatata gcatatattt aaaacattat
    19621 tagtaaattt taaggcacct tcatctccga gtcttttctt tgtctcaaag tacataggta
    19681 tataagttgt agcaactgca gagcctatga ctgctatgat taaaccaggt atatttaatg
    19741 ttaatacaaa tacgtctgca tacatacctg tcccatagaa atttgcaagt acaagttctc
    19801 taaggaaacc taatactttt gaaaacatag tggctgccat aatccacaat gctgctttag
    19861 ctgtttttga catcttcttt ctccttatct aatttaaact ctaaaagtag aacttttaaa
    19921 actgcttata aacattattc taattaagta aatttaacct atgaaaagct tccttataaa
    19981 ttaaaaacta tttcgactca tatctaagtg ccctaaatgt aatcaatagt aataatagta
    20041 ttaatactat acaagataag aatgttagat taaatatgct cttaaataga ctataatcac
    20101 ctactgtata cccaacttca actacatcat ttatattgct atctttcaag aatttatctt
    20161 gtgcacgact taagttatca ctacaaatca gtattggcat attttctttt gctgatacta
    20221 ctcccaaaga aatatattcg tttacatcag atctgctacc aaagtctaat ttagcagtat
    20281 agattttctt tatatctttt tcatcataaa acttctttat taaatcaaca tttgtacttg
    20341 acttatttat ttgctttatt ccttctatat ttttcaagtt agaatcaatc tctgcaataa
    20401 attcttttga atcacctaat gcatatattt ttttaagatt cttacctttt aagaaatcaa
    20461 tttgttcctc aaacccttca tctttagccc atattattgg cattccactt ttagcggcat
    20521 aagaatatac agatatggca ttctctaatc ctgttgttga gctaagtaaa aatgcttcat
    20581 caaatcctac tgttctatac atttctctag ctatctttaa agatgtatca tatctgtctt
    20641 ttcctcttat tctttctact tttattccca ttttttctat attacgttca gcatctttag
    20701 atactgcctt taaaccacct actatcgtaa ccttctctgg acctagttct tttagataat
    20761 ttcttgttac tctaccaaga tttttactct tagttacaac tattggagca ttttttgcat
    20821 atgctaaagg tgttgcactt atactatcta caattgactc ttcatttaca agaattactt
    20881 ctttagaatc cttaaatgct cttttattta attctgtcga aatctctggt atactccctt
    20941 ctatcaattc tcttttggga tttgcatctg caaatacctc tattgtgcta gaaatagaga
    21001 ttaacaaaac taacgataat atagtagaaa tttttttaat cataagttct cattcctttt
    21061 cttttttgta ttttttgaca ataaaaaagc tctttttaaa gagctttttc attgattagt
    21121 aatctaatat tttaaaatta tctagctccg tctcctgtca atactacttt aacagtttta
    21181 aagagtatct taaagtcaag taatatactc ctatttttta tatagtaaat atcttcttta
    21241 agtttttctt caggagtgat ttcgtatcca ccatttactt gtgcccaacc tgtaagccca
    21301 ggttttatag cgagcctgtt gataaatcca ggtatttcct cattgaattg aacagtaaag
    21361 ttcggtctct ctggtctagg gcctattaag cccatatccc cctttaatat attaaacagc
    21421 tgtggaatct catcaattct agttttccta ataaactttc caaccttagt tattctagga
    21481 tcatcttttt cagcccattg tgcgccaaat ttttctgcgt ctgttctcat agatcttaat
    21541 ttatatatat agaatcttct cccgcatttt cctagtcttt cttgcttata agttattggt
    21601 cctttgtcct ctattttaat aagaattcca aatattgcta ttaaaggtaa acctatcaca
    21661 agtcctatta tagataaaac taaatccata attctttggt acaagtcgaa taaaaccgtt
    21721 ccttttacta tgctatagtc aatctcactt gcaatttctt tatctgcccc tggatagctt
    21781 ttaattttag gtatttcaca ctcatcatta gtatatccag acatagttac ctccatattg
    21841 gaataaaaat ttttttaatt cttaatttca acttatttct atcttatttt tgacttattt
    21901 ctatattata ctcaataatg agcagattat caatacaaga aacatcattg taaacaattt
    21961 gattctttac aaaaatatat catctcttgt tatattttac attataacag gagatgatat
    22021 gttttgcttt aatatttttt attttatttt actgtatctt ctattcctat tccaatttga
    22081 gttcccattg tttttacttt ttctgcagta tttaacttat ccatatcgct cttattgtca
    22141 ataaatgcac attcaacaag tattgctgga taattgttat ttcttaacac atataggtaa
    22201 tcttttccat tatcaagtgt tcttgtcttg attcctctat ttttcatgtt aaatttttct
    22261 agtattcttt ttaagatatt tgatgctgca gtcttagttg ttccaccatt tttatctttt
    22321 actttgtaat aaatttccac accattacca gctccattag acgcattgta atgtatactt
    22381 gtaaataaat ctggctttat agtgtttgat aatgctgttc tttctcctaa agccattgtt
    22441 ttgtctgtat ctctagtcat aacaacattt ataccttttg aacgtaaata ttcagtagtt
    22501 gctaatgctg tattaagagt atatttcttt tcttgtgctc caccatttaa tccacttgtt
    22561 gcccctgaat cagaaccccc atgacctggg tctatcacta cagtttttcc tgctgctgaa
    22621 cccgaattat ctggttctgt tggtgcattg tgttttgata aactagatgc tatagaattt
    22681 ataacactat ctttcatccc atcaccaatt tgatatactg ttcctgctgt tttttctgat
    22741 aagtttgtgc tatggtatgc agaaacataa gtctttggag ttataagtat tggagctttt
    22801 aatttagctg ctaaaggtcc tgctgttatt gagtcaacta ttatgtcaga ttttgcaact
    22861 aacattgcac tcaactcttt atctggatag aaagtcttaa ttacatttgc atttgtttca
    22921 tgtctatctg ctccagaaac tctatttttt gaagtaccat tttttgctat tttagatatt
    22981 tggtctataa tttttgatga taaactttga ctaccaccta tgtaataagc ttcttctaaa
    23041 ccttgtgaag ataaccaatt atatgtacct tgtggtacag agtctttatt agctaatatt
    23101 ataggttgtt ggtcttcacc agcttttgat gaaatattaa gagcatcata ttcaccagca
    23161 tatccatttg ctatgtatat tttattaaca tcatgatact tatctatttc ttttgctata
    23221 agtaaagatg tttcatgtct atcaactcca ccaatacgag ttacatttac atttacagca
    23281 gatttaattt gagatactgc cttttgtgaa actgaaccat catcacctat aattattaca
    23341 tcacttggat ttagacgttt taattctgct tttgtacttt ctggtatatc atctttattt
    23401 gcaagtaata ttggtgcatc atatgtagat gcaagtggag tagctgtaat tccatctgct
    23461 gctaattctc cattaactat tacaacctta ctagacccat ctttccaacc ttgtttactt
    23521 atctcaacag cagtttcaaa tctagtagct ccagcaagtt tttttactgg cgcagaagtt
    23581 tttctcatat attcatttac tgcttttgtt gaatttgcat cacttattat aaactcttgt
    23641 gtaacatcat aatccttaag atttaatccc atagactcta agtcttcttt gtttaaaact
    23701 ttctgttcat ctttaccatt tatttttatg ctttgaagtt cccatacatt taagtattct
    23761 gggtctttgt tatagtttat tacaccttca tatgaatctg tcttttcatt gtaatttaaa
    23821 gtagcttcaa gtggcatatc ataacacata aaatttaatg ttattgaatc agcatttaat
    23881 ttttctttaa attttacact tgcattagat ttacttccta acactacact ttttctttgt
    23941 aaatttatat taatatctga attagattct gtatttgcag tagtattttc agccgctaaa
    24001 gctacgtttg gtgccaccat tgctacagac aacattcctg ttgccaataa ttttgttgtt
    24061 tttttcatca tatcccccct aattttttgg ttctttaaat ctttaaatct tttttaatac
    24121 atcttaagct tgttttatat tacataaatt caatattatg taaatctttt ttcattatag
    24181 cacaaaaaat tacaaattgt aaactttttg taatctttta tggcatggtt tttaaatttt
    24241 atcgaggaga aattttgatt atatttgata gtttccgtat atttgaacaa atcaaagact
    24301 ttatcatcta aaaatattgc actaaatttt aataaatatt tttttcttgt acattataga
    24361 cattactata acccagaata ggacatacaa taaagaaaat ataagagaat cccaagttgt
    24421 acctgcccaa ggtgttataa gttcatatgt agtccactct accaaggtag ttggataatc
    24481 gactttgttc gtaagttttg gaacattcca aaaaacatga ctcaatatct caaggcacat
    24541 atatgtaaaa atagggctac ttccaagtgc tattattggg gtaaatattt tacttttatt
    24601 tttgatgtca catataaaat aaaatattga cagtaacata ccataactgc cagccattaa
    24661 aagtacaaat gagctagacc ataatctttt attaaatgga aaatactgat taaagataaa
    24721 agcacctatt agcaggatta tgctcataac cagtatttta aagaattttt tatattctcc
    24781 tatgttttct tttaaaagta cacatcccat agtgcatccc aacatacctg aagaaattgc
    24841 tactatacta gttaaaaaac catctggttc aaattctggc ttgtacaaat gtcccttgaa
    24901 gaaatgtaaa tccactaact gtgcaagaga accttctagc tcaaatccat aaggttttgc
    24961 taatatgtag tacccaacta ttataaatgt agctatacat agaaaagtaa ttattgtagc
    25021 agtacttcca acatttaatt tttttagtag taaatatact aaactagtca caaaatagac
    25081 taatcccata cgttgaagta ctccaagtat tctcacagaa tttaaatcag gatttcctag
    25141 gtaatttaag aaaaatccaa ataatatcaa taagatactt ctcttgaata tactaagtat
    25201 tatactcagt gtggatttat tattttttag tttactattt attgatattg gaattgtaac
    25261 acctaaagat ataacaaaaa aaggaaatgc aaaatccgca agtgttacac catgccatac
    25321 agcatgtctt aattgaggat acattctcat ccaagtccca ggattattgc agactatcat
    25381 gagggctatt gatagccctc ttattatatc tattgattta atcctactat tcgtaagttt
    25441 agaatctaca acattatttg aaatt
//
LOCUS       DAELXX010000056        24609 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.056, whole genome shotgun sequence.
ACCESSION   DAELXX010000056 DAELXX010000000
VERSION     DAELXX010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24609
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.056"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     repeat_region   11..569
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=76..104
                     /rpt_unit_seq="ctttacattccatatagttaatataaaac"
     gene            complement(1400..2272)
                     /locus_tag="KPH29_002106"
     CDS             complement(1400..2272)
                     /locus_tag="KPH29_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG1467697.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
     gene            complement(2666..3244)
                     /locus_tag="KPH29_002107"
     CDS             complement(2666..3244)
                     /locus_tag="KPH29_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG1467698.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            complement(3302..4018)
                     /locus_tag="KPH29_002108"
     CDS             complement(3302..4018)
                     /locus_tag="KPH29_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBG1467699.1"
                     /translation="MRINKYIASCGIASRRKSEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKTYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            4209..5294
                     /locus_tag="KPH29_002109"
     CDS             4209..5294
                     /locus_tag="KPH29_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG1467700.1"
                     /translation="MIIILKENIKYYLIVVFIGILFFKFINTPSNFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEVKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYIDSFIEKNMSNIDFLKTLIPHIQHSLDNLLKEASNFVGKIPKNFLI
                     YTLSISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLYSSTARNKADNIIEFFRTTHD
                     IFYDYLLGRILDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPP
                     IAMTILYSPQKTIWVIAFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFG
                     FIGVFLSVPIAAVVKTYVDKYIDNRLQ"
     gene            complement(5449..6108)
                     /locus_tag="KPH29_002110"
     CDS             complement(5449..6108)
                     /locus_tag="KPH29_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467701.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KYSSKNNDKKSDSNKSDNNNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
     gene            complement(6325..7617)
                     /gene="lysA"
                     /locus_tag="KPH29_002111"
     CDS             complement(6325..7617)
                     /gene="lysA"
                     /locus_tag="KPH29_002111"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBG1467702.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYIEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
     gene            complement(7635..8828)
                     /locus_tag="KPH29_002112"
     CDS             complement(7635..8828)
                     /locus_tag="KPH29_002112"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBG1467703.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHVVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            complement(9189..9806)
                     /locus_tag="KPH29_002113"
     CDS             complement(9189..9806)
                     /locus_tag="KPH29_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467704.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            complement(9976..11676)
                     /locus_tag="KPH29_002114"
     CDS             complement(9976..11676)
                     /locus_tag="KPH29_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467705.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
     gene            complement(12073..12636)
                     /locus_tag="KPH29_002115"
     CDS             complement(12073..12636)
                     /locus_tag="KPH29_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467706.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
     gene            complement(12795..13508)
                     /locus_tag="KPH29_002116"
     CDS             complement(12795..13508)
                     /locus_tag="KPH29_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HBG1467707.1"
                     /translation="MGKVAKILLSLIVIVLVLAGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            13800..15464
                     /locus_tag="KPH29_002117"
     CDS             13800..15464
                     /locus_tag="KPH29_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HBG1467708.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLEQINPNSLEILQGFVEPTQ
                     IKDSKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(15571..16470)
                     /locus_tag="KPH29_002118"
     CDS             complement(15571..16470)
                     /locus_tag="KPH29_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBG1467709.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(16497..17135)
                     /locus_tag="KPH29_002119"
     CDS             complement(16497..17135)
                     /locus_tag="KPH29_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBG1467710.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KVGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
     gene            complement(17472..18470)
                     /locus_tag="KPH29_002120"
     CDS             complement(17472..18470)
                     /locus_tag="KPH29_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HBG1467711.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KRISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFLTSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
     gene            complement(18568..19143)
                     /locus_tag="KPH29_002121"
     CDS             complement(18568..19143)
                     /locus_tag="KPH29_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467712.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
     gene            19459..19722
                     /locus_tag="KPH29_002122"
     CDS             19459..19722
                     /locus_tag="KPH29_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467713.1"
                     /translation="MIKNTKQITVEVNEILLQEISKITNDENNILTDLINESIVNYIL
                     ENKKHNIENKDLEEVIKFYKKENTEKPKSAARAIAEAKTHTLY"
     gene            complement(20151..20528)
                     /locus_tag="KPH29_002123"
     CDS             complement(20151..20528)
                     /locus_tag="KPH29_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG1467714.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
     gene            20869..21981
                     /locus_tag="KPH29_002124"
     CDS             20869..21981
                     /locus_tag="KPH29_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG1467715.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYNDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMSLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
     gene            complement(22189..22665)
                     /locus_tag="KPH29_002125"
     CDS             complement(22189..22665)
                     /locus_tag="KPH29_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467716.1"
                     /translation="MVKKRLVAVVLSLAMISVGNFSYAHSGGHNSSGGHHNKNKSELT
                     RYYCGGHSAHQPNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(22864..24489)
                     /locus_tag="KPH29_002126"
     CDS             complement(22864..24489)
                     /locus_tag="KPH29_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG1467717.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALFNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
BASE COUNT         8223 a         4032 c         2552 g         9802 t
ORIGIN      
        1 cacaatttta atttacattc catatagtta atataaaaca gttgttttta ttttttctaa
       61 agagaaaaaa ctagacttta cattccatat agttaatata aaactttctg tatattttag
      121 ttgtgtttta atattataac actttacatt ccatatagtt aatataaaac atggcagtaa
      181 ctaagaagaa aactatacct tttttattct ttacattcca tatagttaat ataaaacgag
      241 gaaattttat actaatgact gatgctagat ttaactttac attccatata gttaatataa
      301 aactattata aggagcatta tacgaccaaa agccaaagca gctttacatt ccatatagtt
      361 aatataaaac aagcaacagc aagagtcgcg acaacattaa aaacttcctt tacattccat
      421 atagttaata taaaaccttt atttttttct ctatatcgag ttcgccaaat tcactcttta
      481 cattccatat agttaatata aaacagtcca gcgttgtgtg gtcgtaacac accaatccta
      541 ctttacattc catatagtta atataaaacc ccaaaataaa ttaagtattt ccaatactta
      601 tacatataca attctcctaa acttgcagtg agcgagcaat agtgcaattg ataacagtta
      661 tcatacactc tcaacgcttt atatttcaac tattaaaccc tactttatca caaaaatcgc
      721 tcactgcaaa acttttatat ttttattata tcataaaact catactataa aataaaaatg
      781 ggataatttt acaaactaaa agcatctaca ttcttataag tatttattat attctctatt
      841 ccaatgattc ctttttcttt ttattgcatc cttcctatgt aatcgcactt tagctcaaca
      901 agtcttttat tttcattaac aattttttaa actattcaaa atcatcaaat ttttcatctt
      961 atacagttaa atcaaaatac gtgttatatc tctttatcta tatacttttc ttccttccat
     1021 ttctttttct ctttctacat tttcactttg ttactttaca atctcaaggt tattatctgc
     1081 tattaaagtt ttatctttcc aggtttcaca aaaataaatt gaataagtgc aaaaataaca
     1141 gatacaaaac cataactatt ttcttttcta taaaaaaata tatcatcaac aagaaacata
     1201 ataaatgaaa ataactatga ataaatactc tcatttagaa gaaatagaca aagatattcc
     1261 ttaaaattta agcaaaatat tgtttaaata aaaaaactga ctgtcagtta aatgccagtc
     1321 agctatttta tatattttat ccaaacatca ctatatttga acattttaat aattaataat
     1381 acttattaat atacaaactt tactcataca cagaatatct tttccaaatt tgctctaaat
     1441 catcaaaatg ctgtttaata tcttcttttt ccttcattcc aacagatata gcaagggcat
     1501 ttgaaatact aagagctgga actagagaat ctacaaatga agccatatta ctcttaacta
     1561 gaagagtgtt atctgcaagt gatgctactg gcgcaaataa gctatctgtt aatgatatta
     1621 catgggctcc tttttcttta gcataattga caatttgata agatttctta gaataacgag
     1681 ggaaactaat agcaactatt acatcttctt catttattct tacaatctgc tcaaaagcat
     1741 ctcccatatc cattctaatt atatgaacat tgtcaagtat tatatctaag taaaaaccta
     1801 aatattgagc tactacaaaa gaactcctca ttccaagtat atatattttt ctcgctctta
     1861 aaagtttgtt agaagcttct ttaaacgctc tctcgtcaat ttcttctagt gtagacctta
     1921 tattatctat atcacttttt aaaactttgt ttagtattgc aaaatcatcg gaataatcat
     1981 gtgccatttc aactctttgt actgtagtta gtttattttt tatgagttct tgaagtgcag
     2041 cttgtaattt aggatatcca ctataaccta atgcatttgc aaatcttaca actgtagact
     2101 cacttactcc tacggtttca cccaatttac atgcagtcat aaatgcaact ttatcataat
     2161 tatttaaaat gtattgtgct ataagttttt gacccttact caatctcgta tattgagatt
     2221 gtatatttga tatcaaatgt ttactgtcct tcatttcatt tgtttcttcc atttttttct
     2281 ccttttagtt atataaacta ttttgtttta ttaacactgt ctttttctct aaaaatttta
     2341 ttttttatct caatttcatc ttagttttta tcatagcttc ttaaactcat aaaattctaa
     2401 cttttctgta ccttatactt cacatagtat tttcgattaa atttattata ccatctttcc
     2461 ttgcattttt taatatttat ccaatatttc ttttaaattt ttcaattttt actcatttaa
     2521 aagttatcta tcttattttg catatttaga tgtatatttt gttttgagaa taagctgttg
     2581 tatacttatt atttaaagta tttttaatag gtcacactat atatattaac tcttaataga
     2641 atcttgtcaa gttttttagt atttattatg cttttttctt ttcaattaaa atcaattgtg
     2701 gtgggttatt gacttgattt aaaaacttag attctaaaac attaaattca tcttgattta
     2761 aattattgac aaggttatat atgcaatctt tttcttcttg acctccatca tgccctgtat
     2821 atatggcaat gcttattact ccattttctt ttaacatttc taaacttaac tttatagcct
     2881 ttaaagttgt atctggtttg gttataattg aatgtttcgc tcttggaaga taacccaaat
     2941 taaataaaac acatgaaact tcttctttta tatattgatt cattttttca tgcccatcaa
     3001 atatcaactt tactctatct gtatatcctt ctttctctag tttctttctt gtagacttta
     3061 ttgcttcttc ttgtatatca aaagcatata caaagccctt ttctccaacc ttctcagcta
     3121 gataccttgt atcataccca tttcccatag tagcatcaat tactatgtcc ccttcattta
     3181 taatattttc taaataaaat ttatttatat ctgtaatttt agttaaatac tttgcatatg
     3241 ccataatact atttctccct actattaact ttatttcatt ttttctcaat ttatttttat
     3301 cttattttat tgattttaaa tattttattt catcttctgt taggtatcta tattctccaa
     3361 ctttggtgtc ttttaatact attttcccca cagaaatcct tcttaaattt aaaactggat
     3421 gatttatagc tttgcacatt tttcttactt gtctatttct accctcatgt atagttattt
     3481 cacacactga ataatttttt tctttatttt ctttagttac ccttattttg gcaggtgccg
     3541 ttgtgtaatc ttctattttt agtcctgttt cgaattgtct tatctcatca tcacttataa
     3601 ttcccttcac acttgccaca tacgttttat caatttcatg ctttggatgg gtaagcttat
     3661 atgttaaatc gccatcatta gtaagtatta ataaaccact agtttcataa tctaatctcc
     3721 ctattggata tactctttcc cttacatctt taagtatgtc tattacactg ggtctattga
     3781 attggtcttt tactgtagtt atatatccct ctggtttatt taatactata tatatataat
     3841 tttcacttgg ttttactttt ttattatcaa attctactat gtctttttct tcatcaatat
     3901 taaaagataa ctcttttact acacttccat ttacctttat tcttcctcca agaattattt
     3961 cttctgattt tcttcttgat gctattccac aagaagctat atacttatta attctcatac
     4021 ttaatccttc cttatcttaa attatgtgtg tttaatattt ttatcaatgt taattttgct
     4081 acattattat actatactag accttaatta aaaacaagca aaaaaattat ttaattttcc
     4141 taaataattg taatttcttt tcacatacta ttaatatgta atagctgact aaacactatt
     4201 ttaaggaagt gattattatt ttaaaggaaa atataaaata ttatttaata gtcgtgttta
     4261 ttggaatttt attttttaaa tttattaata ctccatcaaa ctttatatct agtattgaag
     4321 gattcttaaa gttttttagc ccatttttat tagctatttt acttgcttta cttttaaatc
     4381 cacttgtgat gctttttgag gtaaaattca aagcacatag acttttagcc atttttttat
     4441 cttatatatt tataggtttt attttagcct ttgccattag gctattaatt ccatcaatag
     4501 ctaatacatt aaatagattg ataaatgaaa tgcctatgta tactgattat atagatagtt
     4561 ttatagaaaa aaatatgtca aacatagatt ttctaaaaac tttaattcct catatacaac
     4621 atagtttaga taatttatta aaagaagcaa gtaattttgt tggtaaaatt cctaaaaact
     4681 tcttaatcta tacacttagt atatcttcta tgctatttaa tatgacaatg ggatttatac
     4741 tatccatata cattatatat gacaaagaaa aaattgcatt gggatttaag agatttctat
     4801 attcttctac tgctagaaac aaggcagata acattataga attttttagg actactcatg
     4861 atattttcta tgattattta cttggtagaa tattagactc tcttattatt ggtataattg
     4921 catttttagg atttcaattt gttatcagaa tagaaaatgc tttattttta gcttcaataa
     4981 tattcttagg taatataatt ccctattttg gtccttttat tggtgcaatc cctccaatag
     5041 ccatgactat attatatagt ccacaaaaaa ctatatgggt cattgcattt ctatttatat
     5101 tgcaacaatt agatggaaat tttatagaac caaaagttat gggtaatcaa gttggtatag
     5161 gagctatatg ggtaatatct gctattttgg tgggacaatc tctatttggt ttcataggag
     5221 tttttctttc agtaccaata gctgctgttg taaaaactta tgttgataaa tatatagata
     5281 atcgtttaca atagcttatc tctactgtga ctttaatttt tagtttaaaa tttaactgtt
     5341 atttattaaa ttatttctca aaaaatatct cttagtaaga aaaaattgct aagagatatt
     5401 ttttataata tataatttgt tcattaaatt tatgcatata ttttatactt attgattatt
     5461 tgagttttga gaatttccac cactatctga accaccagta gtatcttgtg gagttggtgc
     5521 tggtgttgga ttactattat ttggcttttc ttgattttga gaagaactgt tattattgtc
     5581 agacttatta ctatctgatt ttttatcatt atttttagaa ctatatttat tatttttact
     5641 acttccacta taagaagatt tccttgcatc tgattcaact ctatacctat cttctccagc
     5701 cgtatctttt gcctttttct ttctttgttc ctctgcttcg ttttgttctt tttctaattt
     5761 agttaactct tctttctttt tagttgcctc ttctttaata acagaggaac catttttgat
     5821 tttctctata agtttaagtt ccttgattgc accctcataa ttttttaatt cttcaaactc
     5881 cgtcatattt gacattctca tagcttgcat atacatagct cttgcattct cattagtact
     5941 atcttcttcc agtgcagagg ataatgattc catagctttt ttatagttat gtttttctaa
     6001 atatgttttt ccttgctcta ctaaatttat ttcatctttt ttattaaatg aacatcctac
     6061 aagaaatata gataatatcg atattaataa aattactctt cctctcatta aatccctcct
     6121 tgtcatatac tttaaatttt atcataatta taaagttttt aaaatatttt atatttatta
     6181 caagaaattt gttagtaaaa caaaaaataa gcagatatgc tacgaacgat acaataaacc
     6241 aaaaattaat ttagagatac atatcctagc aaatctgctc ataataaact ttaatattta
     6301 atttttatta tatacttttc cattttatat ctctaaagct aataagtcct catagctttg
     6361 tctcttacaa attatcttat catttccatc aaatactgat actactgctg ctcttggtat
     6421 tttattataa tttgaagaca ttgaatatcc atatgcacca gttgatgttg ttataagtat
     6481 atctccactt ttaatatcca ttatgtttat atctccaatt aatatatctc cactctcaca
     6541 acattttcca gctattgtta catgattcat agcctcatga ttaattttat tcacaatact
     6601 acactcataa ctagcttggt acaacgaagg tcttacatta tctgtcatac ctccatctac
     6661 acttacataa accctaacat cttttatatc ttttatagaa cctactgtat acaatgtact
     6721 tcctgcattt gcaactattg atcttcctgg ttctattacc aatgtaggta cttctattcc
     6781 aagctcttta catgaagttt ctgctttagt gattatggtt tcacaaaact cttttatagt
     6841 tttaggttta tctccttcgc tataataaac gccaactcct ccacctaagt caatttctgt
     6901 tagttgtatg ttgaattttt cttttatttc ttttaccaag ttcatcatta tatccactgt
     6961 atctatgtat ggttctacat caaatatttg tgaacctata tgagcgtgaa gacctacaag
     7021 tttaatattt ttatattcac ttaatttttc tattgctcta aataaatctc catttactaa
     7081 agcaaatcca aatttagaat ctatctgtcc agtttttata taatcatgtg tatgagcttc
     7141 tataccagga gttattctaa agtatatctc ttgagtttta cctttatctt cacataatgc
     7201 ttcaagcaaa tctaattcat aaaaattatc cactacaaat ttacctacac ctaaatctaa
     7261 tcccatgttt atttcttctt tagtcttatt gtttccatga aagaaaactt tttccatagg
     7321 aaaatttgat ttataagctg tatataactc gcctcctgaa actacatcta aacacatttg
     7381 ttcttcattt ataagattac acatgtaaag aggtaaaaaa gctttccctg cataagcaac
     7441 cttatttttt ccctctctag ccttaaaata ttctatatat tctctacaat tttgtctaac
     7501 taaagcttca tcaaatacat aaagtggagt ttgatatttt ttactaagtt ctaaacaact
     7561 cactcctcca atatataatt catcattatt tacagtcata gttccatgaa gtttcataat
     7621 agcctcccca aattttatat attaaatact tctgctattt tttctatagc tacttgctta
     7681 tcatcagaat ttatagcaca agttatacga atctctgatg tagttataag ttttatcatt
     7741 ataccatttt cattgaatat cttaaatact ttagcagcaa ctccagaagt atttttcata
     7801 ccaagaccta ctaaagaaaa tttagtgata ttattatcaa tcactacatg ttcttcatct
     7861 gtatatttag atactatctt tttacattct cccaactctt cttttggaac tgtaaatgat
     7921 acacttatct tatctaaaat aggtgcagtt tggcttatca tatctacact tatacctata
     7981 gttgctatat cttcaaataa gctagatata ttttcagctt taaaatcttt tattgttata
     8041 gaactgtcat catcactagt agctaatcct gttattactt tatcctctaa tttcatgtct
     8101 tttccccctc ttatatatgt tcctttttct gtaccacatg tacgtccgac atagatttca
     8161 acaccatatt tttgtgcaag ctctatactt cttgaatgca ttacttgtgc tcctaaactc
     8221 gctaactcaa gcatttcttc atattcaatc tcatcaagtt tactagcctt tgaatatttt
     8281 cttgggtctg taaaataaat tccatcaaca tctgtatata tctcacattt tccatttaat
     8341 ttaaccgcaa gagctacagc agacgtatct gaaccacctc ttccaagagt ggttacatca
     8401 ccatcttcgt tgattccctg aaatccagtt actatcacga catttccttc atctaaacta
     8461 tttttaattc tgtttacgtt tatatcatca atttgagact ttccatgaag accactcgtt
     8521 tttatattta actgataagc attataactt atagctttac atccaagtgc atttaatgct
     8581 attgataata aagatgctga aatcatttca ccagtagaca ttaaagcatc taattccctc
     8641 ttactaggtt cattactcaa ttcctttgct aaagttatat attcatcagt ggattttccc
     8701 atggcagaca caactattac catttgtgga ttttcttttc ttcgttctat tatattttct
     8761 gctattgatt ttattttttc agtatctgct actgaacttc caccatattt ttgaacaatt
     8821 atactcatgc tttctccccc tcaagctaaa ttttgataaa taaaaacgcc caaaggtata
     8881 ctttggacgt gtaaatttca tagaccaagt aaccttgtag ctcaccactt taaaagtgac
     8941 agccttatgc atattctgca taagtccaga aaatttgtcc gtcaacaaaa tttcttcggc
     9001 tatataccct ttcatcaatt atcaatgctt accagctcct attgaatact attgaataca
     9061 gctacctcta ccctaggctc attattttta attaatttat taatatagtt ttatcatatt
     9121 ctatataata ttgcaacaat tattttacaa aatgtggcaa atcgatagtt ttcttttcta
     9181 aagttagctt atatacaatt aaatagttta tttaatccta aatctataaa atttatgcta
     9241 tactcattat catatctctc taaatcattt aattcggata gattatctgg tgtaactacc
     9301 aaaacgtttg gtactttaaa aaatggaatt gtagatttat ctattttttt agaaagattt
     9361 tcgtatttag aaatgtctat ttcattattt aagtcaacat caactaaaac ttgctttata
     9421 ttaccaaatt ggtctgtata ttcaataaat aaatcgtatt tcacatcatc tacactaaca
     9481 tttcggtaaa atctttttac atcacaacct gcagtattga ttctaacaac taattctgat
     9541 cctactagag cctttattaa atcatctccc ttaaaaagtt tactaccgtt taaataatat
     9601 acataagaga ggtctttata attataataa tccacatcac aaggatattt ttttagtgta
     9661 tattcactac acatttttct caaagttttt ttaaaatttt ttttattgta tcttttttgg
     9721 tctccataaa tttttctaaa ttgagactct gtaacacatc taaccctgct aattaaaagt
     9781 aatatctctt tttccatatt cgtcatagta cattctcctt gaagtattat ttgcaaagtt
     9841 gtactaatat tatattaatt ttatgacatt aaatttacaa agaattaaaa taaaatagct
     9901 aatttttata gaattaaatt ctataaaaat tagctatcac tatgttaaat actattatca
     9961 atctttttat ttaaattata cattaccttg attttcagaa tctttggaat ctgtattttc
    10021 atcagttcct gttttttctt ttgctttttt atcttcaaag tttgcaatat ttaggttatc
    10081 aaattctgac atttctatta caccagattc ttcttttgaa tacgatattt tgactttatc
    10141 accaacctga gtcaatggca attcgctaga tacttttgaa gtagccctaa atattatatc
    10201 tttatttgaa tctaaagtta cataataatt agtgttacca cctcgcacat ctgggcttat
    10261 tcttgtcact gtgccttcta atacctcatc ttttaaatca ttatcaagtt ttaaatcatt
    10321 acccttagat tctaatgcat ccttatagtt tcttaatgct tcattcttat cttcacctat
    10381 tccaagtaca ttataatctt caacagatac aaatgcaact ctcttaacaa gaccagcctt
    10441 atcttttaat gatgatacat atgttggctg acccaatata ttatacatta cagggaacgt
    10501 cgcttcataa tttttttctt gaacattacc ttcagctgat ttcattgcag ccgtttcagt
    10561 agctcctggt tgtttatata atctagcttc ttttgttcta gaatcaacta acataaatcc
    10621 aattgtagat tcatctccac ctgaagatgt tatacctgta taccaataag acctgttatc
    10681 attgccataa actaatgatg taccttctgt tggtactaaa actccctttt ctgatataac
    10741 agagtttaaa aatccattaa cataaacacc ccagtcttta atctggtctg ttacaaagct
    10801 ttgtggttgt attctatcca cccatttagg tgtgttttta acatcataaa catttatttt
    10861 tccagtttct gcatcaacag tggctattcc tgttgcattt gctcctccat agccaatttt
    10921 atgttcatat aaacttacaa cccaataagg tcttccttca tcatttattt caagagtgaa
    10981 atctgtcata ccaacattta caataccatg tatgtataaa tgtctttgta aatcctgatg
    11041 taagtaggct tctggttggt aaactatctt tattggcttt ccatctattt cttgaactaa
    11101 ttggacgtct tgaggattac tagctgatac ttttacataa ccacttgtac cacttagaga
    11161 tgttatccat ttaattatgt ctctgtgaac aagaggcgct acccagtaca actctccatc
    11221 tacattttgt atatgaaatt ttcctaattt ggatacactc cctattgcag gaacttctcc
    11281 aagctttttg tcacctaatt tcattgccat atcttcatct acaagacgaa tgtcatttac
    11341 actgacaggg ctaatgtcat cagtaaattt actttcagtt acttttccca atagttctct
    11401 atatgatttt gcatgaagta caggtgtaga agttataaag ggaactacta caatatacac
    11461 aagcaaagct attgctattg aaaaattata tttagccatt ttagatgctc tttcatttct
    11521 atctaacatc atatctagta ttcctgctac aacaaagaaa attattccta ctgaaaataa
    11581 agatgtaaaa tctaatctta gtacaggtaa tttaataaat gcataaatta ttacaattat
    11641 taatgcaact ccatatgttt taagatatcg tttcatttaa tactcctctc taaaaaatgt
    11701 tattattatt attatatcca tatatttaca aaaaataaat atttatcata tttattatat
    11761 attaagatat ctttatatat ttatagttct gcttattcaa ctatatttaa cattatacgt
    11821 gtatgtataa atacttttta tacactgttt gtgctatcca tcccgcacct aaaggagtgg
    11881 gatttttgca ctattctgta ttatatatat ccaaaaatat tataaaaata caaatattag
    11941 cagaaattta aaaactgaag aaagtattct aactaagatt tacaaattct taatcctatc
    12001 gaagttaaac tactatcttc agcctaatta tctagcaata tttttttata tgaaaaactc
    12061 taaatcaagt ttttaaatgt ttgcgatttt tgatacataa gaatgttgtc tagaattata
    12121 ttctgataat aaaccttcat actctaaggt taagccagat aaattataaa aatcatttgg
    12181 cgttacttca aatgcttgtt tatttaaaat gtctatctca cttttttcta acatgtcagc
    12241 aacaaagttt gaacatacat atttatattc agattgtctc tgaatattca ttagtaaata
    12301 tattaatgcc attgtatcgt aatagtaatc ttctctataa tcactaatta attttatatt
    12361 tttatataga ttttcatatt gtaaattatc tacttctaac gaatataccc tacacacagt
    12421 gttttttctt atagcataca atccttgatt tatattttct tcaacaaatc ctccaataaa
    12481 tggattcctt ggatttagcc ttccaaaaga atacattggt tttagatttt catttaatga
    12541 tattgacaca tgtgtataca gctttttact tatattcctt attaagtaag ataatatagt
    12601 tcctgtatat gttgttacaa tatagacttt attcatcatt tccccccaat ctagtaacta
    12661 aatgatatta tacacttaca atttaatatc ataagatatt ctaacataat aaatgtggtt
    12721 ttacaaattt tgattttaag gtactttaat attatataat ataataaaaa tataaatata
    12781 agttttctat atttttaacc aacagatgtt ttcttcaaat taatcagatg atttagtata
    12841 tcttgaacct ttccagagtc ctttaaatat cctaatgccc catttatttg tgattttaaa
    12901 tctatttcta cttccatatc caaatcaaca tttccttttt ttatagatat atttttaagt
    12961 gttattgggt aaagataact tttttccaaa gtaatcttat ttccattaac ctcaaatgga
    13021 acttttttat tgtagttact taatattttt tctagcattt tatcacttag tttaaatttt
    13081 ccaattttca cattctcaag acttacaact aagtttccat cctttaaagt aggtctcaat
    13141 tcaagttcat attgactatt taccaaacca aataccttat atggtcctgc tacctttatt
    13201 ttaccatttt tcatttcaac aaaattattt tcaagttcat taattccatg cttctgcatc
    13261 aatgtatata ttaaattttt aaactcttcc tctgaaacag atatttttcc aaccattcta
    13321 agtggatttt ttactacctc catagaattt atatatgagc cttttgataa atattcttca
    13381 tctatgattt tattattttg tgaactaaca tcatatcttt cttttggtgt caagatatat
    13441 acaacaattc ctgcaagtac taaaacaata acaattaaac ttaacaaaat ctttgcaacc
    13501 tttcccataa tcgtctcctt tacaaatctg tcatcagatt taaaataaat ttagataact
    13561 aacttcacat aatattctta acaatataca tactataata cagtctttta aaaattaatt
    13621 caaccaatct aatgttttgt aaataaactg taaaaacatg tttttagtat aaaagttaat
    13681 attttgcata atactcttaa ttacaccttt atttttttct aatcttggat taaaattaat
    13741 attatgcaat acagtgtagt gttctgtatt ataatcaaat taaataggag gtatagaata
    13801 tgtcaaatga aacgaactca tctaacttta taaagaatat tattataaac gacttagaaa
    13861 caggaaaaca tgacagtatc ataactcgtt ttccacctga gccaaacgga tatttacata
    13921 ttggtcatgc aaaaagtata tgtcttaatt ttggattagc taaggagttt aatggaaaag
    13981 ctaatttaag atttgatgat acaaacccat taaaagagga tgttgaatat gtagagtcca
    14041 taaaagaaga tgtaaagtgg ttaggatttg attggaacga gttaaatttt gcatctaact
    14101 actttgatga aatgtataag agagctttaa tactaataaa aaagggtaaa gcttatgtat
    14161 gtgatttgac acaagaggaa atgagagaat atcgtggtac tctaactgaa ccaggaaaag
    14221 aaagtcctca tagaaacaga actattgaag aaaaccttga tttatttgaa agaatgaaaa
    14281 atggtgaatt taaagatggt gaaaaaacat taagagccaa aatagatatg tcttctccaa
    14341 atataaacct tagagaccca attatatata gaatatctca ttcaacacac cataatacag
    14401 gagataaatg gtgcatatat cctatgtatg cttttgctca tccaattgaa gatgcaattg
    14461 aaggaataac tcactctata tgtactttag agtttgaaga ccaaagacct ctttatgatt
    14521 ggtttgttaa agaatgtgag atggaaaata tacctagaca aatagaattt gctagactta
    14581 atataaataa tactgtcatg agtaaaagga aattaaaaca acttgtagat gagggtattg
    14641 ttgatggttg ggatgaccct cgtgttccta caatatccgg aatcagacgt aagggatata
    14701 ctgcagaagc attgcgcaat ttctgtagtg aaataggggt atcaaaagtt aactctacgg
    14761 tagatagtca aatgcttgat tatttcctaa gagaaaactt acaaccaaag gctcctctta
    14821 ctatgggagt tttaagacct ttaaaactta ttataactaa ttacccagaa gataagattg
    14881 agatgcttga aatagagaat aatgctaaag atgaatctca aggaaagaga ttagttcctt
    14941 tctctagaga attgtatata gaacaagatg attttatgga agaacctgtt aagaaatatt
    15001 ttagattttt cccaggaaat gaagtccgtt taaaaggtgc ttattttgta aaatgtactg
    15061 atgtaattaa ggatgaaaat ggaaatgtag ttgaaataca tggtacttat gaccctgaaa
    15121 ctaagagtgg ctctggcttt acaggacgta aggtaaaatc tacaatacat tgggttgatg
    15181 ctaaatcagc tataccatgt gaatttagat tatttgaacc attaattctt gatgatatac
    15241 ctgaaaatga aggtaaacat ttcttagaac aaataaatcc taattcattg gaaatattac
    15301 aaggttttgt tgaacctaca cagataaaag attctaaacc ttttgataaa ttccaatttg
    15361 ttagaaatgg attctttagt atagataata aatatacaac tgacgaaaaa tttgtattta
    15421 atagaatagt tcctctaaaa agttctttta aaccaggtaa ataatcttac aatcaaaaaa
    15481 tgaagactta aaatataaat tacttctact ataaataaac ttagttagaa gtaatttttt
    15541 atttgagtct tcatcattta ctttaaatga ttaacttttt tcactacaga ttcccataac
    15601 ttctattcca tcttttatgc attctatgtt aagaggaaaa tctggtcctt tttctccgtc
    15661 aatatctgtt ataagtgaat cttcacattc tatatgcacc tttttagttt taaagtatag
    15721 tatttcattt ccattatatt gatctaaata atcacctcta agcacactta tcaaaactgg
    15781 tattgatttt ggtattggca ttcctttaaa aataattaca tccaaatatc catcatctat
    15841 ttcagcctta tatgctagat ttatatttcc tgctgttttg ccattaaaaa tcaacataag
    15901 atacatatct ccatcataat acatttcctc agaagatact tttatcttaa atttcctcat
    15961 atacaatgct tcttctattc cctttatata ataagaaatt cttccaaatg aattttttaa
    16021 gtctgtgttt atcctttgtg atacatctgt aaacatacca gcacttgcaa catttataaa
    16081 gtacttatta ttgactttcc ctatgtctat tttctttggt tttgaattta tgatttcatt
    16141 tatagcttct tttatatcaa atgaaatttg aagagcattt gaaaaatcat ttgcagttcc
    16201 tgtaggcaaa attccaattg gaatttttat gtctaattct ttcattgcat ttactactaa
    16261 atctacagta ccatctccac cagctatcaa tatgtgttca taagaactat caacttcctt
    16321 aaatgcattc tttacaggtt ctttaatagt taatctatag ggtatcaata aataattttt
    16381 ttcttgatat aattctatga tagtgtctaa atttgataat atcttttttt cacctgaatt
    16441 aggattgtat ataagtttta cttttttcat tatttctcct tactcatttt attcttttag
    16501 tatccaaaat cttcatttaa aaaaatcaca tgtattcttt ctggtgttaa ttgtcttttt
    16561 ataagtgtat aactacagca tattcttcct ggacttgaac agtctataca tataccagta
    16621 gtcttacatc cagtatttgt acttaatctt ttacaattgg ctggagcagc tattgttttt
    16681 gttcttttta tagcttcatc tacatcttta actattttat ttactcctgc tataactatt
    16741 actttatcag gtccatatat catagcagca actctattcc cattcccatc taaattgtaa
    16801 agttctccat cctctgttat cgcattagta cttgtaaaat aagcatctgc agaaaatgac
    16861 tttctatata tttctttcat ttcttctggt ttgatatctt ttgcatatct atcaagaaaa
    16921 ttatattttt ctcttataaa atcaatcact cctgtttcaa ataaggtcat agaacctcca
    16981 catcctacta gagaacctac tttaactatt tcttcaattt ttttaagaag ctcttctttg
    17041 ttacttacta aatacccttt catattattg ttttccagag attttattgt tttttctatt
    17101 cttttttcat taagccaatt taaattttga tccattttaa cttccccctt atttttaata
    17161 tgtacttaat tatttataaa ctttattgtc tttataagtt tattttagta catctaaaaa
    17221 aaaatacaat ataaaactta aatagtttta tataattttt tccaaaataa cttactcaat
    17281 caaaaaagga aagtagatgt actattctac tttccctata ttgtatgtag tatttattgc
    17341 ctaaaatcta tatattaatt atatgtttca tattatataa tatcacagtt aattaaaatg
    17401 taatactatc ttctttttaa acatataaat tgtattatta aatttaatat atccaacttc
    17461 aataaatctt attaatttga tgaagaagat gctatagctg cacttgttgc tgccattaca
    17521 catgcaattt gaatagcaac aacaatattt aaaaatatat tattagttat agagcctata
    17581 ttattctcat ttcgtatcaa atctgcataa attgaagcta caatagcaga actaatcatt
    17641 actctattac tttttcctaa agaccaattt ccaaagcctt tttcttcttt aagtttattt
    17701 gaaacatttt ttatctcttc tattatttca tcttctctat aatctaataa tgaaattgct
    17761 cctattattg gatatccata ataattcatt ttacaatcat tttctttaaa tgctttttta
    17821 agttcaatag tctttttaca tttaagcata gttctatcat cactaaataa aagaatttgg
    17881 gataatgctt gtaaatcatt tcctttataa aaaccatttt catttagaaa ttcataacat
    17941 ttttctatgt attctaaatc ttcatctaaa ttttttgaat gaattgcaat aagtgctata
    18001 ttacaatagt catcactaga agttagaaat ggatgttttt tcctcatgta atcatatatt
    18061 attttcattt ttgatataca tgtaagataa tccattttct ctctattttc ataaactatt
    18121 gatgctgtaa gtggtaaata tgtatcgccc caaaactttt cacttttaag ttctttgtat
    18181 atatctaaaa tcatcttaaa actactttct ggatttgatt caaacgaaag tagtgttgcc
    18241 atataaaaca tattatatcc tctaaaatta gagaataatc cagtattttt tttaataagc
    18301 tctattcgtt cttccactaa tctaggactt attctcttgt tcttaagagt aaatgcaagt
    18361 gcacaactat gttgtatcaa tcccatttgc caagaacctt taacttcatt taatgctttg
    18421 taattttcta ttaataagtt tacttttcca tttataaacg attgttccat attgccctcc
    18481 tagtaatata tctcattgta ctaatagttt tatatttatt atatatatat taaatactac
    18541 aatcaataca tattacttca aagaacatca ttcttcaaca ataatgtagc cttttttgta
    18601 atgtcttccc atattattga ctctatcata gtactctgat tcactttcat tactccattt
    18661 gggcacaaat acatctattg cattagaatt actaaaatac ttactagacc ccctatccat
    18721 aacagttttt aattttccat ctacaataat cttagttcca aaactcaagt gattgtttgc
    18781 tacaaaacca tcttctaaat catctcctgt agcagttatc gctccatatc cattttcttg
    18841 attaagactt gtgtaataac taacttcaaa agttataggt actaacttct ttattttaat
    18901 acctaactga ttttgtagtt tctccttttg tactttcaac ttatactcat cactattttt
    18961 ataatcttcc ataccttgat tatacttgga attaaagctt ttacctgact ttactatagt
    19021 atctatattt actatagatt ctttacaatg atctgcatct atttttgttt ctccatttac
    19081 caataatgga gttaaacata cactacatac aacacatgta atcaatgctt ctttaagctt
    19141 cataaattcc ccccttatta ttttgatata ttagcatatt taatattata tgtctatatt
    19201 tagatttaaa taccacaccc atttgcttta agaagttgtc ttatatttaa ttcaccttaa
    19261 acacttataa atcctctatc ttagaatcat atagaataaa gcttttatcg ttaaatattt
    19321 tttattaatc ttttaaaatc taaagctact ttatttaatt ttctcttttt tagaatttaa
    19381 gaattaaaat tctataattt taaaacatat tgaatttatt tttttgagat gatataattt
    19441 tcttaagagg tggttgatat gataaaaaat acaaaacaaa ttacagtaga agttaatgaa
    19501 attttattac aagaaatatc taagataact aatgacgaaa ataatatttt gacagatttg
    19561 ataaatgaat caattgtaaa ttatattctt gaaaacaaaa aacataacat tgaaaataaa
    19621 gatttagaag aagtgattaa attttacaaa aaagaaaata ctgaaaaacc taaaagtgca
    19681 gcaagagcta tagctgaagc aaaaactcat accctgtatt aatcaaataa aaaagttcct
    19741 aaaaactagg aactttttta tattaataat tattaatata tctcaatatt tatctcaaat
    19801 ttatcaatat aagcatgttt aatacaatct attgtatact tatcttaccc tagaaataat
    19861 caaaacaaaa attatgccct ttatatctaa caattaattt acatgattaa tatacaaaaa
    19921 ttctgacttt taaattcgta tacaatatca aactaaaata atactaatga caaacccaat
    19981 ataaattaaa ttcaaacagg tttgatttta catcaaactt attgtattaa tattaaataa
    20041 caagacaaaa tatttataaa aattacaaat tttacataaa actttatatt ttattcattt
    20101 aactaattat tgtaaaaact atttatttta tgataattaa ctacaccaaa ttattcatct
    20161 tttttaatat ctttaaccca tttttcaaca gagttcaatt cttcttcatt tacccccttt
    20221 ttgaacaagg atttcataaa actctcaata gaacttccat gcatattatc taaaaaagtt
    20281 tttgtttcaa aatgtaaata ctcttcttct ccaataagaa ctgtataatg tgtatgtcta
    20341 tcaatctttc ttgcacttaa gaattgctta tttactaatc ttgataaaag tgttagtgtt
    20401 gtagtttgct tccaattata tagtttttcc atagcttcaa tgacatcttt tgatgctact
    20461 gtaaaatctg aactccaaat aaatttcatg acttttaatt ctgctggtgg tatcttagta
    20521 atagtcatat gattttacac tcctctcaaa cttctacata attaaatact atattaatag
    20581 acttttcgag tcaaaggttt tatttaaaat atctcatcca aatttattaa tacaaaaaat
    20641 ttatattttt attcaaaatt aaacacacaa tctattaata taaagataaa tatctattct
    20701 ataagtataa cagattatca aattaaaatt ctaatatttg acataatata tacatttttt
    20761 aaataaatat atacaatgta ttaaaatgta tgcaactcta tttcaaaatt attttgtgat
    20821 atatatatgt tataataaat gtataaaatt ttgattggtg ggttatatat gaatgcattt
    20881 attagaaaaa gaaataaaaa ctatgttgtt tacctggaat ttgaagatga aaagacaggt
    20941 aaaagaaaac aaaaaaatat gggtactttt gataaaaaaa gggaagcaag taaaagacta
    21001 aatgaggtaa aagaaagctt atataatgat ggttttttag taccaagcga aattacagtg
    21061 tcagaatttc tattagattt tctaaataaa tatagtgaaa atatatcttt agctacatat
    21121 aataattatg ttagtatttg taaaaattac attaatccat ctattggaaa atataaaata
    21181 caagacttac atccggttca catacaaaac tacatagata aactatctta taaactaaat
    21241 cctcaatcaa ttaagattca tataaatata ttgaaacttg caataaaaag agcttataga
    21301 ctcaaattaa taaaagaaaa tgttatggat agtatagagg ctccaagata taaaaaattt
    21361 aaaaatgaga tttatgatag agaacaaatg ataaaattat tagatttagc aaaaaataca
    21421 gaaatggaat tacctattaa tttggctatt ggattaggtc ttagaatttc agaagtttta
    21481 ggtctaactt gggataatat tgattttgaa gaaaatacaa taacagtaaa taagataact
    21541 tcccgaatta atggttctgt tattctcaaa gagccaaaga cagaaagctc tgttagaaaa
    21601 atatcttctc ccaaagaatt aatgtcttta ttaaaagaat ataaaataaa acagaataaa
    21661 aatttattaa aatcttccat tagaaataat aataatttgt tgtttttcaa taagaaatgt
    21721 gaacctattg ctgaagatgt tatgagtaaa aaatttaaaa gatttttaga aaagaatgaa
    21781 ttacctcata ttagattcca tgatttaaga cactcacatg ttactttact tataaattct
    21841 aaagtaccta taaaggttat atccgagaga gtaggacact caaatataag tactactcta
    21901 agcgtttatt ctcatgtact taaagaaatg gataaagagg cttctgacaa aatttctgaa
    21961 agtttattta atgctaatta atattaattt atcttagctt taaattagct tagcttattg
    22021 agatagattc attaatatta aatgtatcta tctcaaatta ataactactt aaataatctg
    22081 ttttatttta atttatcgcc tcagttatta tactttttta tgaaaaatta tactttacaa
    22141 taaaaaagat atttccttat agaaagtatc tttttaaata ctattttatt atatacttgc
    22201 taacattgga atatctaaca tcttcttcat tacatcatct actttttttg ctttatattc
    22261 attgtatcca attaaatatc ctttttcata tgaacttttt aaaatctctt tttcatataa
    22321 attatcagat ttggcttctg tgacacttaa attataaagc cctttacacc catcttcata
    22381 acctttatta tacccttttt ctgcaaaata gtttttatga tttttttcgt tatgtactga
    22441 agatattgtt actccagaat tttcttttgt actagcataa ggacaaaaac ctttatttgg
    22501 ttgatgtgct gaatgtcctc cacaataata acgtgttaac tctgatttat ttttattatg
    22561 atgacctcct gatgaattat gccctcctga gtgagcatat gaaaaattcc ctacactaat
    22621 catagctaaa ctcaatacta cagctactaa ccttttctta accataatct attcccccca
    22681 aaatgttata tatttttatt atatcatcta ctatattata tttaaattct taatatacaa
    22741 aatattactc gattctgcta ataaacatat ttaaacaaca taaaaaacct cctaatattt
    22801 gattaaaaat ttacacactc atgcatagca tgtatcaaat attaaaaggt tttatactaa
    22861 taattattta tcttttccat acattttagc taattggtct tgtatctctt tactttctaa
    22921 atactcatca aagtccattt ttctatccat tattccattt ggagttatct ctataattct
    22981 atttgctata gtagatataa attcatggtc atgagaagta aataaaagca ctcctgggaa
    23041 cttttcaagt cctttattta cagaagttat actttctagg tctaagtggt tagtagggtc
    23101 atctaataca agtacatttg catttgaaag cattagctta ctaagcatac atctaacctt
    23161 ttctcctcca gatataactt gagcttcttt taatgcttct tcacctgaga ataacattct
    23221 acctaaaaat cctcttataa aactttcact tttttcttca gagaactgtc taagccagtc
    23281 aaccaaagaa tattctactc cgtcaaaaaa cttattatga ttttttggaa gataatcttg
    23341 agaagtagtt actccccatt tatactctcc actgtctggt tcaagttctc ccataactat
    23401 gttgaaaagt gcagtagtag ctatttcatc tcccataaat actactttat catctctatc
    23461 caatctaaat gacacattat caagaacctt aaccccatct atagttttag ttaaattgtg
    23521 tacttctaaa acttcattcc caatttctct tgcaggtgta aatcctacat atggatatct
    23581 tcttcttgat ggttgtatat cttcaacttc taatttctct aactgtttct ttcttgatgt
    23641 tgcttgttta gcctttgaag cattagaact aaatctagct ataaattcct ttaattgagc
    23701 tatcttttct tcagtctttt tattttggtc cttagccagt tgtaaagcca attgacttga
    23761 ctcataccag aaatcatagt ttccaacgta catttgtatt ttaccaaaat ctacgtcaac
    23821 tatattagta catatttgat ttaggaaatg tctatcatgt gatactatta taactataga
    23881 ctcttctaaa tccattataa aattatttaa ccagtttatt gatttaaaat ctaagtggtt
    23941 tgtaggttcg tccattaata gtatttcagg cttaccaaaa agtgattgtg ctaacaatac
    24001 cttaaccttt tccccaccaa ctagttcctt catttgtttg taatgcatat ctttcgttat
    24061 gccaagaccc ataagtattt tctcagcatt agtttctgca tcccatccgt caagctctgc
    24121 aaactcacct tcaagttcgg cagcttttat cccatcttct tcattaaagt cttccttcat
    24181 atacaatgca tctttttctt tcattatgtt ccaaagtctc tcatgacctc ttataacaac
    24241 atttaaaaca gtttcttctt catattcaaa gtggtcctgt tttaaaactg acattctttc
    24301 tttatcagtt atagatatac tacctgtatt aggctctatt tctcctgata atatttttaa
    24361 aaaagtagat tttcccgcac cattagctcc tattattcca tagcaattac ctttagtgaa
    24421 ttttaagtta acatctttaa atagttcctt atcaccaaat ctaagtccaa cacctgtaac
    24481 ttgtaacatt aatatatttt cctttctttt tgctcataat ttattttatc ataaaacttt
    24541 gataacattt ttattatata ttatcacgta aatagtatat tttttatact aaatttgtga
    24601 aatatttat
//
LOCUS       DAELXX010000057        24466 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.057, whole genome shotgun sequence.
ACCESSION   DAELXX010000057 DAELXX010000000
VERSION     DAELXX010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24466
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.057"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            32..148
                     /gene="rrf"
                     /locus_tag="KPH29_002127"
     rRNA            32..148
                     /gene="rrf"
                     /locus_tag="KPH29_002127"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            268..459
                     /locus_tag="KPH29_002128"
     CDS             268..459
                     /locus_tag="KPH29_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBG1467718.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            614..2023
                     /locus_tag="KPH29_002129"
     CDS             614..2023
                     /locus_tag="KPH29_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG1467719.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKM
                     DTTEILGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEISEETNVLKGISISNVIDIIDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPAFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIRIFKDKVDSNINIYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTAEDFSSLETALNNVVKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKICGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQVIDLEDI"
     gene            2154..2843
                     /locus_tag="KPH29_002130"
     CDS             2154..2843
                     /locus_tag="KPH29_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBG1467720.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKPMER"
     gene            2846..3781
                     /locus_tag="KPH29_002131"
     CDS             2846..3781
                     /locus_tag="KPH29_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBG1467721.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMSGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3778..4671
                     /locus_tag="KPH29_002132"
     CDS             3778..4671
                     /locus_tag="KPH29_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBG1467722.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4749..5495
                     /locus_tag="KPH29_002133"
     CDS             4749..5495
                     /locus_tag="KPH29_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBG1467723.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRASSRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            5496..6329
                     /gene="rsmI"
                     /locus_tag="KPH29_002134"
     CDS             5496..6329
                     /gene="rsmI"
                     /locus_tag="KPH29_002134"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBG1467724.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRTLKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFVFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEVRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6352..7056
                     /locus_tag="KPH29_002135"
     CDS             6352..7056
                     /locus_tag="KPH29_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBG1467725.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLNTYIEEGINKLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(7244..7672)
                     /locus_tag="KPH29_002136"
     CDS             complement(7244..7672)
                     /locus_tag="KPH29_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBG1467726.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7709..9139)
                     /locus_tag="KPH29_002137"
     CDS             complement(7709..9139)
                     /locus_tag="KPH29_002137"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBG1467727.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            9271..9855
                     /locus_tag="KPH29_002138"
     CDS             9271..9855
                     /locus_tag="KPH29_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HBG1467728.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            9871..10392
                     /locus_tag="KPH29_002139"
     CDS             9871..10392
                     /locus_tag="KPH29_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HBG1467729.1"
                     /translation="MEIFSNLLIPIIILYIVVYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    10458..10704
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10788..12725
                     /gene="metG"
                     /locus_tag="KPH29_002140"
     CDS             10788..12725
                     /gene="metG"
                     /locus_tag="KPH29_002140"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBG1467730.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTDFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12755..13525
                     /locus_tag="KPH29_002141"
     CDS             12755..13525
                     /locus_tag="KPH29_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBG1467731.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGICYEEVCRVTKENAKKFFNIK"
     gene            13862..14293
                     /gene="phnG"
                     /locus_tag="KPH29_002142"
     CDS             13862..14293
                     /gene="phnG"
                     /locus_tag="KPH29_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HBG1467732.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            14308..14883
                     /gene="phnH"
                     /locus_tag="KPH29_002143"
     CDS             14308..14883
                     /gene="phnH"
                     /locus_tag="KPH29_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HBG1467733.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTKLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTVSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSTINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            14886..15971
                     /locus_tag="KPH29_002144"
     CDS             14886..15971
                     /locus_tag="KPH29_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HBG1467734.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVELLEEDEKKNRDFRDSFINELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKLEEVIDDITRNSLRFPVSRSAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKVKHPIYEDEEIIIGDMLVTECEIVSKASQNK
                     TENSKTNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIK"
     gene            15991..16860
                     /locus_tag="KPH29_002145"
     CDS             15991..16860
                     /locus_tag="KPH29_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HBG1467735.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            16853..17719
                     /locus_tag="KPH29_002146"
     CDS             16853..17719
                     /locus_tag="KPH29_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1467736.1"
                     /translation="MSKINVSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQRELKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            17760..18473
                     /locus_tag="KPH29_002147"
     CDS             17760..18473
                     /locus_tag="KPH29_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG1467737.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMAKGTISESGVA"
     gene            18474..19643
                     /locus_tag="KPH29_002148"
     CDS             18474..19643
                     /locus_tag="KPH29_002148"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HBG1467738.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDRIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPANGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVLNTIVNGKTVYKREV"
     gene            19647..20483
                     /locus_tag="KPH29_002149"
     CDS             19647..20483
                     /locus_tag="KPH29_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBG1467739.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSSLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            20484..21479
                     /locus_tag="KPH29_002150"
     CDS             20484..21479
                     /locus_tag="KPH29_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG1467740.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLDVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            21497..23185
                     /locus_tag="KPH29_002151"
     CDS             21497..23185
                     /locus_tag="KPH29_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBG1467741.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     DFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYIIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYIPQTQTA
                     SISILKLDEIGYIGPSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            23197..24264
                     /locus_tag="KPH29_002152"
     CDS             23197..24264
                     /locus_tag="KPH29_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG1467742.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
BASE COUNT         9752 a         2736 c         4381 g         7597 t
ORIGIN      
        1 aacttaatag aaatattaag cataaagatt atgtggttac aatagcagag aggatacacc
       61 tgttcccatt ccgaacacag aagttaagct ctctagcgct gatggtactt ggtgggaaac
      121 tgcctgggag agtaggacgt agccacgtaa ttttttttat tttttgtcaa aaaaaacaaa
      181 aaatacgtat ttattttttc atttcgggaa aactaaacac aggaattatg gactttataa
      241 aaatttaatt cttgaggagt gatataagtg aaaaaagttg ctttaatttt agctataata
      301 ggtgcgctta actggggtgc aattggtatt ctaggtacag acttaatagg tagtatcttt
      361 ggtggaacat atgaaatggt aagtagaata atatacttca ttgtaggttt agctggatta
      421 tacttaatac caagtttaat gtctgatgat agagaataat ttatgctttt atgttaaata
      481 cctcattcta aatgtttaga atgaggtatt taattttata aaaatattaa tttgtttctt
      541 ttttaatggt aaaatagata agaattgaag ttttaaaaag aagtattatg taaaaaagga
      601 gtactattaa tatatgttta ttattaatga attaaataag attgtaaatg aagaagtaat
      661 atcttttcat atgcctggtc ataaaaaggg acaggtttat aaaaagttag gatataaaga
      721 tatactaaaa aatttgtata aaatggatac aactgagata ttaggaacag ataatttaca
      781 ctttccggaa ggaattataa aaaattcaca aaaaaaagca tcaagagtat ttaaaagtga
      841 ttatacttgc tatttagtaa atggtagtag ctgtggaata caatctgcaa taatgtcagt
      901 atgtagtcct aaaagtaaaa taataacaaa tagagactgt catcagtctg ttataaatgg
      961 atgtatttta ggagatatag atatagagta tgtgccttgt gagataagtg aagagacaaa
     1021 tgtcttaaaa gggatatcta taagtaatgt gattgatata atagataaaa acttggatgc
     1081 aaaagcagta ctgcttactt atcctacata ttatggaatg acatatgatt tagaatacat
     1141 atgtaattat gcacatagca aaaagatggt agtgatagta gatgaagcac atggtgcgca
     1201 tttagggctt agtgagaggc ttcctaaaac agctcttgag caaggtgcag atatagttgt
     1261 acagagtaca cataaaactc ttccagcatt tacacaatcg tcaatgatac atatacaggg
     1321 agaccgagtt gatatagata aaatattgaa catgttaaga attacagaat cttcaagccc
     1381 ttcatattta ttattatctt ctctggaatt agcagtagat atatatgaaa ataaaggaaa
     1441 agaattaatg gaagagcttt taaataatat tagaattttc aaagataaag ttgattcaaa
     1501 tattaatata tatgacacaa atgataaaac aaaaatattt atatcatcta aaaacatggg
     1561 tttaacaggt tatgaactag aaaatatttt gagaaacaaa tataacatac aagtggagtt
     1621 atctaattat tatggagttc ttttaatatg tacaatagga aatactgctg aggatttctc
     1681 tagtttagaa acagcattaa acaatgttgt aaaggaagac tttaaatttg aaaagttaga
     1741 aaataaaaaa taccctgttg ttattccaaa gaaaatatta acacctagag aagcatttta
     1801 taaaactaaa aaaagtgtta aaatatatga cagcataggt aaaatttgtg gtgaatgtat
     1861 agttccatac ccacctggaa taagtgtaat atctcctggt gaagtaataa gtaaagaaat
     1921 aatagactat ctaatgtttt gtcattctaa aggcatgatt gtgagtggat tgaaagatgt
     1981 agatttaaat tttatacaag taatagattt agaagacatt taaaaattat gatataaaag
     2041 tagatagaag tagatagttt aaaattgtga agagaaataa atagactgct tatatagagc
     2101 tatctaataa aagtactaaa aaagtaaaaa gtgtttgtat ggaggatatg aaaatgaaag
     2161 gtaagttaat aattatagag agtggttcag atgcaagtgg aaaggctact cagacagaaa
     2221 tattatatga aagattaaaa aaagaaggct atatgattaa aaaggtagaa tatccaaatt
     2281 atgaatctcc atcatctgcc ttagtgaaaa tgtacctaaa aggagacttt ggtaaaaatg
     2341 catcagatgt agacccatat atagcatcaa ctttttttac agctgatagg tatgcatcat
     2401 ttaaaaccga ttgggaggag ttttacaatg aaggtggaat aattatcgca gatagatata
     2461 caacctctaa tatggtacat caggcctcta aaatggatat ttgtgataga gaagagtatt
     2521 taaattggtt agctgattat gaatttaatt tatttaagat accacaacct gattgtgtta
     2581 tatttttaga tgtgcctatt gaatttagta aaaagcttat ggagaataga aaaaataaaa
     2641 ttacagggga agaaaaaaag gatattcatg aaagtgacat agagtattta gagaaatctt
     2701 acaacaatgc tttatatata gcagataaat atgattggaa aaaaataaat tgtgttttag
     2761 ataacaaatt aagaagtatt gaaagtatac ataatgaaat ttataaaata gttcttgatt
     2821 caataaaacc tatggagaga taaatatgta ttttgaaaat attatagggc aagattttgc
     2881 aaagaaatat ttaacaaatt ctataaagaa aaataaactt aataatgcgt atatgtttga
     2941 aggaatggat ggaataggaa aaaagaaatt tgcagatgaa ttatcaaaac tattacttga
     3001 ttatgaaaat ctcgaaaata gtccagacta tgtactcata aaaccagatg gaaatagtat
     3061 aaaaatagct caaatcagaa atttacaatc agatatagta ataagacctc ataaagacta
     3121 taaaatatat ataatcaata atgctgaaaa aatgactgtg gaagctcaaa atgcactttt
     3181 aaagacgcta gaggaacctc caaactatgc tataataatc ttagtcacaa acaacaagga
     3241 atctttatta gaaactataa aatctagatg tgatataata aagttctcac ctataccaat
     3301 tgaagactta aaacgttatt tgataaatac aggtatagaa gaagaaagag ctcaattatt
     3361 ggctattttt tcgagaggaa gtattgagaa tgctctaaat ctatctcagt cttctgagtt
     3421 ttcaatgatg agagaagata tacaacagta tattcagata atgttagata aaaacatagt
     3481 ggagatttta aatataccaa acaatatgga aaaatataga ggtaagatta tagctttatt
     3541 agatatgatg ataaactatt ttcgagatat catcctatta aaagaaaatg taaacaagaa
     3601 catgttaata aatgtagata agctagtttt cattcaaaat atgagtggaa aaattagtta
     3661 ttctcaattg tccaagatta ttgatataat agaagatgca aagagtaaaa taaaaagcaa
     3721 ttgcaatttc aatataagca tacaagttat gtctttaaat atatacgagg tgattaaatg
     3781 ataaagatag taggtataag gtttaaaagt gctgggaaaa tatattattt tgaccctgtt
     3841 gattttaata tagagcaaga tatggatgta gttgtagaaa ctgcaagagg cttggagtat
     3901 gggaaagtag tcgttggtcc aaaagatatg gatgaaagcg aattagtttc tcctttgaag
     3961 ccaattataa gaatagctac agaagaagat aaaaaagtat atctagaaaa taaggaaaag
     4021 gcaaaagaaa cttttgagtt gtgccaacaa aaaataaaag aacatgagtt aacaatgttt
     4081 ttaatagatt gtgagtatac gtttgatagg aataagttga ttttttactt tacagcagaa
     4141 ggaagaatag attttagaga attggtaaaa gacttagctg ctatatttaa aactagaata
     4201 gaacttagac aaataggtgt cagagatgaa gctaaatcta taggaggtct agggccttgt
     4261 ggtagaaaac tttgttgttc atcttggtta ggtgattttc aaccagtttc aataaaaatg
     4321 gcaaaagacc aaagtctatc tctaaatcca acaaaaatat ctggaatatg tgggagatta
     4381 ttctgttgct taaaatatga gcatgatgta tatagtgaag caatagaaaa aatgcctgtt
     4441 gtaggtgcat tggtgcaatc agatgatgga aaaggaaaag ttgtagaagt gaatccttta
     4501 ttagaacaaa ttaaagtgga atttcaagac aagacagtaa agatatacac aagagaagat
     4561 ataaaaatac ttagagagcc taaaaaatgt gatggctgtg gtggtaaatg taaggataat
     4621 gatggattag atgaagccac actaagagag ctaaagaaat tagaagatta aatttaaggt
     4681 agggcttttc ctaccttttt taaagcaatt aatgcttaaa gttattaata tttgaggaga
     4741 atttatatat gcaattaaaa gattcagaaa gaatagatga tttacaatta aaaggcctta
     4801 aaattataca agatactaat ggtttttgtt ttggtataga tgcagtttta ttagccaatt
     4861 ttactaaaat aaaaaaagat gccaaagtag tagatttggg tactggaact ggaattatac
     4921 ctattttgat agctggaaaa agtgaagcta aaaaaattat tggcgtggaa atacaagaag
     4981 atgtatatga aatggcaact cgttctataa aattaaatgg attagaagaa agagttgaga
     5041 ttataaatga agatataaaa tacatagaca aagtcttaga cataaatggt taccatgtag
     5101 tcacatcaaa tcctccatat atgcatattg atggaataaa aaatccaaat gataaaaaag
     5161 caatatcaag acatgaagta aggtgcaatt tggaagatgt aattagagct tcatccagat
     5221 tggttatgcc tagaggaaaa ttcttcatga tacatagacc aacaagattg gttgatataa
     5281 taactttagg gagaaaatat aaattagaac caaaggttat tcaatttgtg catccaagac
     5341 ctcaaaaagc accgaactta gttttggttc aatttgtaaa agatggaaga ccagagctta
     5401 aaatattgga cccattgtat gtttatggag aagatgggaa ttatacaaaa gaattaaaag
     5461 caatatataa taataaagat ataggagagt tgtaaatgag tgggaaattg tacatatgtc
     5521 caacaccaat aggaaaccta gaagatataa catatagaac tttaagaaca ttaaaagaag
     5581 tagacttgat tgcagcagaa gatacaagac atagtataaa acttttaaat cattttgaaa
     5641 tctcaaaacc actaactagt tatcatgaac ataataagga ttcaaaggga gattatttga
     5701 taaataaact aattgatggt gaaaatatag ctctgataag tgatgcaggt atgcctggta
     5761 tatctgaccc aggagaagag ataataaaac aagctataca aaataacata gagatagaag
     5821 tattgccagg tgcaacagcc tttgtaacgg ctttagttgg ctctggtatg gacacacata
     5881 gatttgtttt tgagggcttt ttagatagag ataaaaaagt taggaaagca caattggaag
     5941 aattaaagga agaaagtaga actataattt tctatgaatc accacataga ttgaaggata
     6001 cactaaaaga tatgcttaaa attcttggta ataggaatat ttctataaat agagaactta
     6061 caaaaaaata tcaagaaata ataagagaaa atatagaaac taccataaaa atatttgagg
     6121 aaaaagaagt aagaggagag tttgtattaa tagtagaggg ttttcatggt gagaagtcaa
     6181 agaaagaaaa ttatgatggg cttacagata gggaatatgt tttaaaacta attgaaagtg
     6241 gtatgaacaa aaaagatgca ataaaaatag tttgtaaaga tagaaagtta aagaaagatg
     6301 tggtttataa acaagtttta gatttataaa acctgaatct gaggtgattt tatggaaata
     6361 gggaaaatta taatagagag tttaaagagg gaagatacac ataaaatatt tatagaatta
     6421 gataataagc atgaattaga aaaaataatt cctaaaataa gctgtatgaa gtctgtaggt
     6481 gaatgtaaat atcatgtagt aaattgtttt gaacattcta taaatgcatt aaaagaatta
     6541 gaaatagtat taaatgataa ggattttttt ccaatacatc taagaaaaca tgtgtcaaac
     6601 tatttaaaca catatattga agaaggaata aataagttac atgtattaaa gttgggaaca
     6661 tttttacatg atataggtaa gcctgatagt atgaccttag atgaaaccgg aagagttcat
     6721 ttcacaaatc atgaaaaaat aggtgctcaa ataattgaca atatgggaat aaagttagag
     6781 ttatcaacag agacatatag acttataagt aaatatgtaa gatatcatat gatacttttg
     6841 tctctatata agaaaaatga tttgagtagg aaagaattaa ttaatgtatt taacttagta
     6901 gatgaggaca ctataggtgt aataatgctt gggtatgcag atatagtgtc tacagtaaaa
     6961 ttattgggca agagtggaga agtcagtgtg ttaaagacat atatggaata tattttaaca
     7021 aattacttgt ataagagtaa ttatacacat gtataaattt agttaatata attgagtttt
     7081 agatgattaa ttgaagtttg aaatatacgg atttcaaaaa cataaaagaa gttgtctttg
     7141 ttgtaaaatt atgaaagaca acttttttta tatcaaaaat tgaaatttta ttaaggatac
     7201 ttaatggtct attaattaat aaaccattag tatttaattt atattacaat attgattttg
     7261 cttgtattgg cgttgatagt ataacagagg tcttagtaga accaattttc tttatagtat
     7321 caatcacatt ttctagctcg tacatatctt ttacaattac ttttaaaagt aagcaatcat
     7381 cacctgttat atggtgacat tctacaatcc tagggtcctt tgcagctgac tcaataaatt
     7441 ctgtatatcc attgcttgga agagaaatat gaataaatgc ctttataact ctgcctaatg
     7501 aatctgggtt gacaatagct ttatatcctt ctataacacc agactcttct aatcttttga
     7561 ctctttctga aactgcagga gaagttaaac caactatttt tcctaagtcc ttcatagaaa
     7621 ttctcccatc atcctgtaaa atttctatga ttctgtaatc tgtaacatcc ataaataaac
     7681 agtccccctt aaataaaaat aattaatact ataaaacttt tttagaatct atcatttgtg
     7741 tttttaaata ccaaagattt ctagataaat caaccttata aatgtgtggt tttgaaagtc
     7801 ttttgtattg ttcccagata gtacttaatt catattgtac atgatttttt atttcaagaa
     7861 cagctttttt attatatttg catctgcctt ttatatacaa tggtttaaga agctctttaa
     7921 ctttgtaatt tgtaaatacc tttgttttcc atgtataagt aggatggaag atttctaaag
     7981 gtttagactc atctataact tcatcatgta acataatcaa atctgcctct gccttattgt
     8041 cttcgttata tattcttata actttcttgt aaccaggatt atttattttt tctggattct
     8101 cagaaatctt tatcttaggt tcaagaactc ctttttcaca agatgcagct aatttataaa
     8161 ctcctccaag agaaggtgaa tcgaaagatg taatgagctt tgtacctact ccccatgaat
     8221 ttatagtagc tccttctgct ttaagtgatg ttatggtata ttcgtctagg tcattagatg
     8281 cagtgatgct gatatctgaa aagccagcgt tatctaattg ttttttagct tctttagata
     8341 aataagctaa atctccagag tcaagtctta tacccatagg tttgtatcct ttttcagaaa
     8401 tatttttgaa tacttttatt gcattaggaa ctccactgtt taatacatca taagtatcta
     8461 caagtagtag gcatttatct ggataaacat cagcatatgc ttggaatgct tcaagttcat
     8521 tatcaaattt ttgtacccaa ctgtgagctt gtgtaccaat tataggtata tcaaacattt
     8581 tccctgctag tacgtttgca gttccagcac aaccaccaac tacagcagcc ctagctccat
     8641 atatccctgc atctggacct tgtgcacgac gtaatccaaa ttcaaataca gggtctcctt
     8701 gagctgcaaa gcaaactcta gaggctttag tagcaataag agactgaaaa ttaactattg
     8761 ttaacaatgc tgtttcaata agttgagctt gatataaagg tgccttaaca gtaattaatg
     8821 gctcattagg gaacatgata gttccttcct caacagcata tatatctcct gtgaatttaa
     8881 attcttttaa aaattttaaa aacttgtcag aaaataaatt taagtttttt aagtactcta
     8941 aatcttcatc tgaaaaatga agattatcta tgtattcaac aacctgatca ataccacaaa
     9001 ctatagtata ccctccatca caagcgtttt ttctaaaaaa catatcaaat acaacaatgt
     9061 cttcatgaat attcttttca aaatagccat ttaacatagt aagctgatat aaatcagtaa
     9121 gaagtgttag atttctcatt atttctctcc ttaattatta ttaaatatta aaaattgtaa
     9181 aaactcccac acttatacta aaacataata tagtacaact ttcaagtata aagtattacc
     9241 taactatcat aaaattattt aaagaggagg atgattataa taggaaaaat atggttttat
     9301 atggtatcga taggaataat aggaagcttg atttctaaaa atatgggtga attaaataaa
     9361 gttatattaa gtgaaacatc aaaaggtata gagtttgcaa taagtctagc tggtatcatg
     9421 gcactctgga tggggataat gaatatcgca aaagaatcag gtttaattga aaagataggt
     9481 caaaaactaa atcctataat gagaagatta tttccttcta ttccaaaggg tcataaagca
     9541 atgtcttata tagttatgaa tattgcttta aatatgttgg gtgctggaaa tggtgcaaca
     9601 gcatttggtt taaaagcaat gagtgagctt caaactttga ataacaagaa agatacagcc
     9661 actaatgata tgattatgtt tatggtaatc aatatatcat ctatacaaat aattccattt
     9721 actataatca aattaaggat ggatatgggg gctcaaaatc caagtgaaat aatttttacg
     9781 actttatttg caaccataat atctacagca gtagcaataa taacatgtaa attttttcaa
     9841 agaagatata gataaagggg atgtagatac atggagatat tttcaaactt acttatacca
     9901 ataataattc tgtacatagt agtttatggt aaatataaaa aaatagatgt gtatgatagt
     9961 tttgtaaaag gagcaattga tggattaaaa gcagcatggg atatcctacc atatataata
    10021 ggtatatttt tggcaatagg aatattcaaa actggtaaag gtcttgatat gttagaatgg
    10081 ctatttacac caatagcaaa tatgatgagt ataccaaaag aattaatagg tctaataagt
    10141 gtaaaacctt tatctggaag tggtgcttta gggatgtaca gtgaacttgc aaatagagtt
    10201 ggaataggaa gtttagtaga aaaaatgggt gctacgattg taggttcttc tgagactatt
    10261 ttttacacaa tggcaatata ctatggaagt ctgaagataa aaaatactag acatacatta
    10321 tcatgtgcta tgattagcca tgttgcaggt gtcatagcag ctgtttttat ttgctatgta
    10381 atatttgtat aatttgttga caatatggga aaaattttat aatatagtga ttatatagaa
    10441 acaaatagta atacaaatta caagctatga aaagaagagt aaataaaggt tttttctatc
    10501 agagagctag gttaggtgaa agctagtgtc aaaagcttta ttgaagatgg tcttggagct
    10561 ttttatctga gcaagtgtgc tagggtaaaa cggttggaca cgttatagtc tactaaggta
    10621 tctataaaat gtttctacat ttcatgtgaa acataactat tatagatgaa ttagggtggt
    10681 accgtgaata atctcgcccc tatgttaact cataggaggc gattttttat tatttaaatg
    10741 aaagaatcat atgattacga acgataaaaa attaggaggt aaataacatg agcaaaccga
    10801 gtttttatgt aacaacacca atatactatc caagtggagg attacacata ggacatactt
    10861 attcaacagt agctgcagat acaatagcta gattcaagcg tttctgtgga tatgatgtaa
    10921 agttcttaac aggaacagat gagcatggtg aaaaaattca aaagaaagcc atagaacaag
    10981 gaatgtcaga gatagagtat cttgatggaa tgataaaaga tataaaggcc ttatggaata
    11041 ctatggatat atcttatgat gattttataa gaactacaga aaaaagacat acagacataa
    11101 tacaaaagat atttactaaa ctatatgagc aaggtgatat atataaaggt gaatatgaag
    11161 gtagatactg tactccttgt gagagtttct ggacagaatc tcaattacta gaaggaaata
    11221 aatgccctga ctgtggaaga gaaacatatt tagtaaaaga agaatcttat ttctttagat
    11281 tatctaaata tgaagacaga ttaaaagagt tatttaaaga tgatagtttc tgttttcctg
    11341 ctgctagaaa aaatgaaatg gtggcaaact ttttagacaa aggattagaa gatttaagcg
    11401 taactagaac gacttttgac tggggtataa aagttccttt tgatgaaaaa catgtaattt
    11461 atgtgtgggt agatgcttta tgtaactata taacagcatt aggttatatg actgataatg
    11521 atgaagaatt taaaaaatat tggcctgcca atgttcaaat agtaggtaaa gaaatagtga
    11581 gattccatac aataatatgg ccagcgcttt taatggcttt aggtctagaa gttccaaaac
    11641 aagtatttgg acatggatgg atactatttg ctgatgataa aatgagtaaa tcaaaaggta
    11701 atgtggttta tccagaacct ataatagaaa gatatggaat agacacactt aagtacttct
    11761 tattaagaga gtttgcattt gggcaagatg gaagttatac tcatagaaat tttgtaacga
    11821 gaataaatta tgatttagca aatgatttag gaaacttaat aagtagaact gtagctatgg
    11881 ttgaaaaata caataatggt ataataccaa cagcaaaagt ttcaactgat tttgatgctg
    11941 acttaaaaga acaagctgtt tcaactagag aaaactttga agctgaaatg gataaaatgc
    12001 aattccatga agcattagag agtgtatgga aattagttag aagaactaac aaatatatag
    12061 atgaaactat gccttgggca ctagctaaag atgaaacgaa gaaagatgaa ttagacacag
    12121 ttttatataa tttatgtgaa tctataagaa taatcgcaac actaataaat cctataatga
    12181 atgagactgc taataaaata tatgaacata taggaataaa aggtcaagat gatataacta
    12241 catgggaaag tacaaaaaca tttggtctta ttggagaaaa tgtaaaagta tttaaaggtg
    12301 aacctttatt cccaagatta gatgtagaaa aagagataga agaattaact aaaatgttct
    12361 ctgggaaacc tccagtagaa gaaaaacctt tagagcataa agaagaaatt actattgatg
    12421 atttagacaa aatagagctt agagtaggga aaataataag ctgtgaaaaa catccaaaag
    12481 caaataaatt attagtatca caagttaaga ttggaccaga aacaagacaa atagtatcag
    12541 gaatagctga atactataag ccagaggatt tagtaggaaa agaggttaca gtggtatgta
    12601 acttaaaacc agttaaatta agaggtgtag aatctcaagg tatgatatta gcagcaggtg
    12661 atgatggaga cccatatgtt ttaccattta cacaaggggc taaagatgga tgcgaggttc
    12721 gttagtaggt aattaaatag taaggagatt aaagatgtta tttgactcac atgcacattt
    12781 aaatgatgaa agttttgatg aagatagaga tgaacttatt ggttcattaa aagataaagg
    12841 tgtagatttg gttgtaaatc caggagcaga tatagagacg tcgataacag caattgaact
    12901 tgcgaaaaaa tacgacttta tatattcagc agttggggta catccacatg atgtatcaaa
    12961 actagatgat acagcaatag aaactctaag aaaacttgca actgagaatg aaaaagttgt
    13021 agctattgga gagattggat tagattatta ttatgattat tctccaagag aagaacaaaa
    13081 agagtggttt aagaagcaaa ttgaattagc taatgagcta aaacttccaa taataataca
    13141 tgatagagat gcacatggag atacttttga gataataaag aatactaaaa atcctgaaat
    13201 aggatgtgta cttcattgtt atagtggaaa tgtagagtta gctagagaat atgttaagat
    13261 gggttgttat atatcaattc caggcacggt tacttttaag aataataaaa aaactagaga
    13321 agtagtaaga gaaatacctc tagaaagatt attcatagag actgattcac catatatgtc
    13381 accagagcca catagaggaa agagaaacaa tccttctcaa gtatcttttg ttgctgataa
    13441 gatagcccag gaaaaaggga tatgttatga agaagtatgt agagttacaa aagaaaatgc
    13501 aaagaaattt tttaatataa aatagacaaa ttaaatctta taatgatata tataataagt
    13561 cattataagt ataggatgag agtgcctcaa aatgagtttt ttagttcatg aggcactctt
    13621 tttttatgaa atcaaatata ttttcatcat ttcaacaatg aaaaagaagt ttatcatatt
    13681 ttgagacagt ttgtttttat ttgtttaatt catattttaa aatatatttt cttaacattg
    13741 ttaaaaaaga atttacaaga tattcattag aattaactca actctaatat tcacttaact
    13801 taagtggttt attatattat taaagagaag taaacgaaag gtagattaag gaggctaaat
    13861 tttgtataca gttgatagta ctaagatatt gataaatatg aacttagaga aactagaaaa
    13921 gatatactca atgataaaag atacatcaag tatttctata attaaagaac ctaacttggc
    13981 tacagtaatg gtaagagcta atgaaagtgt aaaaaataca acatttaatt taggagagat
    14041 attagttact gaatgtagtg taaaggtaga tgaaagcttg ggttacggaa tagtttcaga
    14101 aaacaataat aagaaagcca tttacttagc agttatagat gcagttttac atagcaataa
    14161 cagtaaattt gatgagttaa agaattacat aaataaaagt gtatatgaag agaacttaag
    14221 gtatgaacaa gagattattg atgaatttag cttgattaat aaaacgaaag ttcaattcaa
    14281 tgctatggat taaataggag gagaatcatg gtaaattata cacaagaaat atatagaaag
    14341 atgctagata gtgcttcgtt tcctgggaaa ttaaataata tagataatat aaatatagaa
    14401 aataatacaa aactattgaa tggagcaatt ggtatagcaa tcactttatt agaccaagaa
    14461 gttaccttct atatagaaaa ttatactcaa aaagatataa aaaatataag agcgcttaca
    14521 gtgtcaaatc aaatagatta tagaaaatca gattatatat ttttagatat taattcagaa
    14581 gtggatatat tacagattaa aataggttct tttgaatatc ctgatgaaag tgcaactata
    14641 gtacatcaag taaatgattt atcaacgtac tctcaaacta agtatataaa gcttgatttg
    14701 agtggtcctg gtataaaatc taaaaatagt atatatataa atggaattag agaagaattt
    14761 atagaaagat tatctaccat aaataaagat tatccaatag gaatagattt gattttagta
    14821 gataaaaaag gagaaattgc atttatacca aggtcatcaa aattaagttg ggaggtattg
    14881 tagaaatggc ttatgtagca gttaaaggtg gagaagaagc catattacag gctaaaaata
    14941 tacttgagtt ttatagggta aaaggagata gcttgcctat agatgtaaag cagattgagg
    15001 aacaaatgtc ttatgctgta gacaaagtaa tgtctgaagg aagtttatac aacaaggaac
    15061 tatcagcatt ggctataaaa caagctcaag gtgatatatt agaagcctct ttctatgtaa
    15121 gagctttaag gtcaacaatg cctagaatta catattcaga acccataaat acagaagaaa
    15181 tgagaataaa aagaagaata tcagctactt ttaaagatat accaggaggt cagtatcttg
    15241 gggcaactag agactatgaa aatagaatat taaatgtaga attattagaa gaagatgaaa
    15301 aaaagaatag agattttaga gatagtttta taaatgaatt agaaacagta gatgaaatcc
    15361 ctgaatttac aaaaatatct aatatattta gaaaagaaaa tttattacaa gaagaagttt
    15421 gtgaaaacaa attggaagaa gttattgatg atattacaag aaatagttta agatttccag
    15481 tttctaggtc agcaaagtta caggcattga ctagaggtga aagtggagta gtgtcaggtt
    15541 ttgcttatgc tattgtaaga ggttttggtg atgaacatcc atatataagt gaacttagaa
    15601 ctggtaatgt atttataaaa gtaaaacatc ccatctatga agatgaagaa ataataatag
    15661 gtgatatgct ggttactgaa tgtgaaatag tttcaaaagc atcacaaaat aagactgaaa
    15721 attctaaaac taattcggaa gataaaaata agatgtcttt aggatatggt ctttgtatgg
    15781 ggaataatga gaataaggct atatctatgg ccatactaga caaatctttg gagccaaata
    15841 gtgaaaaact agcacaagat gaagaatttg tattgcttca tatagatgga atagattcat
    15901 taggatttat atctcacttt aagcttcctc actatgtaga ttttaaagcg gatgtagaaa
    15961 gaataaaata gtttgtggag gtaagtttag gtgatatata actatggttt tttagacgaa
    16021 aacacaaaaa aagaaataag aagaaagata ttaaaagcaa tatcaatacc tggttatcaa
    16081 gttccctttg gttcaagaga gttgcctata gctaagggat ggggtactgg aggtttacaa
    16141 ttaacactat cattaattgg taaaaatgat gtagtaaaag taattgacca aggaagtgat
    16201 gattctacaa atgcttgtaa tataaggagc tttattgcaa gtgtaagtaa tgtagaaacc
    16261 acaaaagata cattagaagc tacattgatt caaacgagac atagaatacc agaagaaaaa
    16321 cttaaatcta atcaaatact aatatttcaa gtaccaatac cagaaaccct tagaatagta
    16381 gaacctagtg aagtagaaac aagaagaatg cattcagaag aagattatag taggatgtgg
    16441 gtatatcttt atgaagatat agttagattt aatgatatat ctatagcagt tgaatatcca
    16501 tgtaaagtaa atgatagata tttaatgaat ccatcaccta tacctagatt tgatataaaa
    16561 aaattaaata tgtcagacaa tttattcttg tttggtgcag gtagagaaaa aagaatttat
    16621 gctattcctc catatacaaa ggtagaacct ttagagtttg aggattataa gtttgaagag
    16681 gaaaagtttg aaggaaaata ctgtagttta tgtaacagta caaatacatt tttagatgaa
    16741 gtatatgaca gtgacactaa tgaaaagtac tatagttgct cagatacaag ttattgtgaa
    16801 aaagttagat taaaaaataa cagtatagat gtgacaatag gaggtacttg gaatgagtaa
    16861 aataaatgtc tctaaaagat tagaaaatat agacaagcaa gtattaatag gaagaaatat
    16921 caatcttatt tatggagatg ggtgtgaaaa gtgttttgaa agcacaggat atgaaagcaa
    16981 taatatatgt aagtattgtg gttctatagt tgcatgtaat aatgtaaatt tagaacttta
    17041 tgaaggagaa gtccttggaa ttgtaggtga atctggttca ggaaaaagca ctttattaaa
    17101 aatactgttt ttccaagaac atgcaaatag tggagaagta tatatatccc attacaataa
    17161 agacacaaat atactagagc tttcagacca aaaaaagaga tacataaaga atcattttat
    17221 gggtattgtg tatcaaaatc cccaccttgg tttaaattta gactttagta gtggtggtaa
    17281 tatagcagaa aaattattga tggcaaattt atataatgtt gaaaaaataa gagatagagg
    17341 caaagaactt ttaataaaaa ctaatatacc tgtagaaaga atagatcata aaccaaaata
    17401 ctttagtggg ggaatgcaac aacgagttca aatagcaaaa gctctttcaa ataaccctcc
    17461 aatattgcta ttagatgagg ttacaacagg tttagatgtt tctgtgcaag cggaagtatt
    17521 ggatttaata agagaaatcc aaagagaact taagatttct atgatagttg tatcccatga
    17581 ctttgatgtt ataaagatgc ttgctgatag gacaattgta atgaaaaatg gaagtgtgat
    17641 tgagtctggt cttacagacc aaataatgga ggacccacaa cacccatata ctcagcaact
    17701 tataaattca ttgctataaa tatcgtttgt aaataagcaa aatgggggtt tttaataaaa
    17761 tgaaagaaac tatacttgaa gtaaataatt ttagtaaaga ctttgagtta catgcattga
    17821 acaagactat aacggcatgt agtaatataa gttttgaagt ttcaaaaggt gagtttttag
    17881 gaataatagg taaatctgga gcaggtaagt ctaccatatt aaaaagtatc tataaaacat
    17941 atataccaac aactggagaa ataattttta attcagaaat atatggtaat gtagacctgg
    18001 ctaaaattgg agaccgagaa atgataaatc ttagaaagaa agaaatagga tatgtatcac
    18061 agtttttaaa gacacttcca cgaataactg caattgaact ggtagtacat tctttaattg
    18121 agagtgggtt tgaaaaagat tgttcgtatg atatggcaaa agatatactt aaccaatttg
    18181 aaataaagga aaatttatgg gatgcgtatc caaataattt ttctggtgga gaaaaattaa
    18241 gattaaattt agctcaagct atggttaaaa agcctagact tttgttactt gatgaaccaa
    18301 ctgcatcact tgataatcaa tctaaaatat atgtaaaaga aaaaatgttg gaacttaaaa
    18361 gtcaaggaac tacaatgata ggaatttttc atgatataga gtttatggaa actgttatag
    18421 ataagacctt tactatggca aaagggacta taagtgaaag tggggtagcc tagatgataa
    18481 aactaattca taatgcaaat attgtattag aagatatgat tttagaaaat ggtcatttaa
    18541 tcatagaggg agatacaata aaaaaagtat caaatgatag gattgattac gcatttgact
    18601 ttaatgagat agatgaaata atagatgcaa aaaacttata tgtaatacct ggtattatag
    18661 atattcacag tgatgccatt gaaaaggaaa tagagccaag accaagtaca ttattaccat
    18721 ttaatatggc attttatgaa ctagataaaa aactacctgc aaatggaata actacagttt
    18781 atcattctat atctttaggg gatggagtag gagttagaag tattgataac tctttaaaga
    18841 tgattaaaaa tattgattca tataaaaata tagatagtaa gagtataaat cataaagttc
    18901 atttaagata tgaagtgttg tattatgaag gtttagaaaa ggttttagaa ctattagatg
    18961 aaaataaaat agactactta tcaataatgg accattctcc aggacaaggt cagtatacaa
    19021 atccaacatt ttataaagag tatgctacta aagtgtgggg agttacagaa aattatgtag
    19081 atacatggtt agatgattta gtaaatctgc atgataattt agattggaat aaaatagcta
    19141 atattatagg tatagccaaa actaaaaata taaatgttgc atcacatgat gatgatacat
    19201 tagaaaaaat ggatttcata aaaaatatag gcataaatgt atctgaattt cctataactc
    19261 ttgaagttgc aaaacattca aaaaaattgg gtgttctaac ttgtttaggt gcaccgaata
    19321 ttgtaagagg taaatctcat aataacaatt taaaagcaat ggatgctata ttggaagatt
    19381 gttgtgacat tgtgtgttcg gattatcttc catcagctat gataaagtct atgtgtatag
    19441 ttgcagaaag aataaataat ttaaataaag cagtcagttt atttacctca aatcctgcta
    19501 aagcagttgg tatatatgat gaaagaggta gtataaaaga aaataaaaaa gcagatttag
    19561 ttttagtaga tattgatagt gaatatccaa aggttttaaa tactattgta aatggaaaaa
    19621 cagtttataa aagggaggta taaaaattgc taacagagtt acattgtcat acaagtatat
    19681 cagataataa ttttactact gaagaaataa taaaaaaagc agcagaaaag aatataaata
    19741 tcttagcaat aacaaatcat gacacattat ctggattgga tgaagctctt gtgcttggag
    19801 agcattatgg aataacaata ataccaggta tagaaatttc agcctatgat tataaaaata
    19861 gaaaaagagt tcatattctt ggatataaca tagatttaaa cagtcaagaa ataaaaaatt
    19921 tgtgcaatcc aatggtatgt gacagacata aagcatctgt taagatggta aataaaatta
    19981 tagatttagg atacaaaata tctttggaag atgtaaagaa atattcatca aagactggaa
    20041 tatttaaaca acatataatg aaagctctta tagataaagg atatacagat aatatatatt
    20101 caagtctata taaagaattg tttcatagag gaaacggcaa ggtatatgta tctttaaaat
    20161 atgtggatta cagagatgca ataaaagcta taaaaaattc tggtggaatt tgtgtgttag
    20221 ctcacccggg tcaaatggat aattttagcg ctattgaaga gatggtaaaa gtaggattag
    20281 atggaataga agtatatcat ccatctcaca acaaagaact agaaaaagag tcgctttctt
    20341 atgctaagaa gtataatcta gtggtcacag gaggctcaga ttaccacgga ttttattctc
    20401 aacataatta tgaacttgga agcaagagct taaataaaga agatttattt aagtttaggt
    20461 tagctttagg aggggtatta taaatgtctt ttttacaagt aaaaaatgta ggtaaaagtt
    20521 atggacaagt taaggtgtta aaagacatat ctattgatat tgaaaaagga gagtttattt
    20581 gtttactagg tccaagtggt tgtggaaaaa gtacactgct tagaataatt gcagggcttg
    20641 aggataaaca tggtggaaaa ataataataa atgataagga tatgactaat tcaccacctg
    20701 aaagtagaaa ttttggtata gtttttcaat catatgcatt atttccaaat atgaatgttt
    20761 acaaaaacat agcctttgga ctagaaaata aaaacataag taaatctaat attgataaaa
    20821 aagttaaaga agttctagac gttgtagaat taagtggata tgagaaaaaa tatccatcac
    20881 aattatcagg tggacaacaa caaagggttg cattagctcg tgcgattgct ttagagccag
    20941 actttttact tcttgatgaa cctttatctg cattagatgc aaaagtaaga cttaaactta
    21001 gggagcagat tagaagttta catagaaaac taggcattac aactataatg gtaacgcatg
    21061 accaagaaga agctctttgt ttagctgata agatggttgt tatgaataga ggagaaataa
    21121 tacaagttgg aacgccaaag gaggtatata aaaatccaga aacacctttt gtagctgatt
    21181 ttataggaac aataaatttt atagatgatg gtattaataa gatagctatt agaccagaag
    21241 atataaaagt agagtctaat agagattcta aagataagga tataaaagtg ggagaaattt
    21301 tagatatcga atttagagga tttaactata gaattacagt tgagtatcgt tctaagcaaa
    21361 tgaaattaga tgtagtttcc aaagttgcag aacaaatgaa actgtgcata ggttctaaaa
    21421 taaactttaa gatacctaaa gaaggaattg tacaatataa atcagaggga tgtgcttaat
    21481 agctaggggt gaataaatga taacatcaaa aaataaaaga agaataagtg atagcacaat
    21541 acaaaaatat ttcttgattt tttcggtaat tgtactaata gtatttatat tatttccatt
    21601 agtatcatta ttaaaaaatg catttatgga tacaagtgga aattttatag gaatttcaaa
    21661 ctttagtaaa tatgttgaaa atccaagtct tatagcatca tttaaaaatt caatttttgt
    21721 atctacagtc tcatctttaa tatcactatg tttagcattt atatattcat acgcaataac
    21781 aagaacaaat ataaagttta aagatttttt taaaactatg ggaatgtttc cactttttgc
    21841 accaactatg ttatatggta tttcacttat ataccttttt ggaaataagg gaatctttac
    21901 aaccatgggt ctggaagtac cactatatgg tccattagga ataattattt ctcagattct
    21961 atttacattt ccacaagctt ttttaatatt atctgtagca ctttcgatgg ctgattatag
    22021 actttatgaa gcagcagata gcttgggtgc tagtgaattt aagaaatttt gcaaaataac
    22081 aattcctggt ataaagtact cattacttag ttcattcttt gtatccttta tacttgcatt
    22141 tacagatttt ggagcagcta aggtagtcgg aggaaactac aatgtactgg ctactgacat
    22201 atataaacaa gtagttggac aatttaacat accaatggga gctactgttt ctatggtaat
    22261 gttaattcct gtactaatag catttacatt agataagatg gcaagtaaga aacaaggtat
    22321 gaccataact gcaaagtcta tgccatataa gattaaggaa aataagttta gagatagagt
    22381 atttttaata gtatgtagtt ttataacatt gctaattttt attttaatat tcgtttcagt
    22441 tataggttca gttataaagt tatggccata taatttaagc tttacaatgg aacactatac
    22501 atttaaaggt attttgggaa gtggaataaa aacatactta agcagtttga aaatatcaac
    22561 tttaacagca atatttggaa ctatatttgt attttttagt gcttatataa tagaaaaggt
    22621 ggataagttt cctaaattaa gacaaacagc ttacctttta tcaatgatac caatggcctt
    22681 acctggctta gttttaggta tatcatatat aacttttttt aactcagcaa gtaatccatt
    22741 aaatttttta tatggaagta cagcaatatt agttatagta aatgtagttc atttttattc
    22801 agtttgtttt ataacctcaa attcatcttt aaaaatgttg gataaagagt atgaattagt
    22861 tgcaaaatct ataaatattc cattctataa agtatttttt aatataacag ttccgatgtc
    22921 tataacatca atacttgaaa tagtagtata ttattttgta aattcaatgg ttactgtttc
    22981 agcattaata tttttatata tacctcaaac tcagactgca tcaatatcaa tactaaagtt
    23041 agatgagata ggatatatag gaccatcagc tgctatggca gtattagtgc tattaacaaa
    23101 tataatagta agacttttgt acgaatttgt aacaaagaaa ttaaaaaata acacacaaaa
    23161 atggcagcaa aaagatgttg cataggggag gaaaaaatga aatttaaaaa gctagtttct
    23221 ttaggagtat tatcaacact tttattaagt gtaataacag gatgtagtac tgattcatca
    23281 ggttcagatt caaagtctga tagtaagaca aaaggggatt taacagttta cacagcaatt
    23341 gaagaagata gtatagagcc atatttagct acatttaaag agaaatatcc agatattaaa
    23401 ttaaatattg ttagggcatc aacaggagat ataacagcaa gattattagc agaaaaggat
    23461 aatcctcaag cagatgttgt atggggagtt gcagccacaa gcttattggt tgcagatgac
    23521 caggggatgt tagaaccata tgctccaaaa ggttctgaag aaatagagtc tgcatttaaa
    23581 gatgataaag aagttccttc ttgggttggg atagatgctt ggatgacagg tataacagta
    23641 aacactaaag aattaagtga taagaacatc ccagttccta gttcttacga agatttaata
    23701 aagccagaat ataaaggtct tatatctatg cctaatccat cttcttctgg tacagggtac
    23761 ttaacagtat cagcccttat acaaattatg ggggaagaaa aagcatggca gtatatggat
    23821 aagttgcatg agaatattgg tgtatacact caatctggtt cagctccagc tacatcagca
    23881 gcttcaggag aatatcctat tggtatatct tttggatata gaggtataaa attaaaagag
    23941 gaaggctatc cagtagaagt agtatttcca aaagaaggtt ctggatggga tatagaagca
    24001 aatgctcttg tgaaaaaaga taatataaaa gaagcatcaa aagtattttt agattgggct
    24061 ataagtaaag atgcaatgaa tgaatactct aagaactatg cagttactac tatttctaca
    24121 ggaaatccaa taccagaagg attccctaaa aagccattag agcaaatgat agataatgat
    24181 ttaaaatcag cagcaaaaaa tagagaagat atactaaata aatggatatc aaaatatgat
    24241 ggtaagacag aaaaagagag ttaagtaaaa ctatacaaat ggagtaaaaa gaaaaaattt
    24301 ttttaagtag attttattat aataatataa gttgagaata atataggtca agaaaagtga
    24361 atattctata aaaatgtata gagtaagtat accattcagt ttgaatggta tattttttat
    24421 ttttagagat taatttagaa atagatatac tacagtttaa aatagt
//
LOCUS       DAELXX010000058        24463 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.058, whole genome shotgun sequence.
ACCESSION   DAELXX010000058 DAELXX010000000
VERSION     DAELXX010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24463
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.058"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            192..3140
                     /locus_tag="KPH29_002153"
     CDS             192..3140
                     /locus_tag="KPH29_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG1467743.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEAISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPLEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            3118..3582
                     /locus_tag="KPH29_002154"
     CDS             3118..3582
                     /locus_tag="KPH29_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG1467744.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            3695..3997
                     /locus_tag="KPH29_002155"
     CDS             3695..3997
                     /locus_tag="KPH29_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG1467745.1"
                     /translation="MGVTNVTKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            4059..5423
                     /locus_tag="KPH29_002156"
     CDS             4059..5423
                     /locus_tag="KPH29_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425524.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBG1467746.1"
                     /translation="MEGLYMEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGL
                     TVGVGFIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALI
                     IPIGLVVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFAAVANMVIV
                     LVMADLTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQ
                     KKFGLFGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLP
                     VSEAAQEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVL
                     PFADLAVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFK
                     MPEGATMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKL
                     SIEE"
     gene            5458..5931
                     /locus_tag="KPH29_002157"
     CDS             5458..5931
                     /locus_tag="KPH29_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467747.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYINFNLNIYSL
                     LIIVCTYGIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYEIKDIEKIRIREFYNKKI
                     YLKINDGDLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLRFRNKKFENKNI"
     gene            6103..6753
                     /locus_tag="KPH29_002158"
     CDS             6103..6753
                     /locus_tag="KPH29_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBG1467748.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTFKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            6927..7859
                     /locus_tag="KPH29_002159"
     CDS             6927..7859
                     /locus_tag="KPH29_002159"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBG1467749.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEDSIFIG
                     KNQNPYQEVHHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYRKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
     gene            8133..8846
                     /gene="ispD"
                     /locus_tag="KPH29_002160"
     CDS             8133..8846
                     /gene="ispD"
                     /locus_tag="KPH29_002160"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBG1467750.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDENCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            8843..9328
                     /locus_tag="KPH29_002161"
     CDS             8843..9328
                     /locus_tag="KPH29_002161"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBG1467751.1"
                     /translation="MRIGLGYDVHKLTENRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    9423..9631
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            9695..11410
                     /locus_tag="KPH29_002162"
     CDS             9695..11410
                     /locus_tag="KPH29_002162"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBG1467752.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFDRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDQCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            11532..12977
                     /locus_tag="KPH29_002163"
     CDS             11532..12977
                     /locus_tag="KPH29_002163"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBG1467753.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKTKGVAVEVDDRDNYT
                     TGWKFNEWEMKGAPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            13417..14898
                     /locus_tag="KPH29_002164"
     CDS             13417..14898
                     /locus_tag="KPH29_002164"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBG1467754.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLVEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEITEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            15119..15274
                     /locus_tag="KPH29_002165"
     CDS             15119..15274
                     /locus_tag="KPH29_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467755.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            15419..16816
                     /locus_tag="KPH29_002166"
     CDS             15419..16816
                     /locus_tag="KPH29_002166"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBG1467756.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKIFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            16801..17214
                     /locus_tag="KPH29_002167"
     CDS             16801..17214
                     /locus_tag="KPH29_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBG1467757.1"
                     /translation="MEKDIKMEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRK
                     VNDLHKLAIKYVKAKAQATIIHEIEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKH
                     ATGFEALVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            17321..17416
                     /locus_tag="KPH29_002168"
                     /pseudo
     CDS             17321..17416
                     /locus_tag="KPH29_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452247.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase (FAD)"
     gene            17722..18552
                     /gene="thyA"
                     /locus_tag="KPH29_002169"
     CDS             17722..18552
                     /gene="thyA"
                     /locus_tag="KPH29_002169"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBG1467758.1"
                     /translation="MSLADKIFIDMCNNILENGVSSDGEVVRAKWDDGQPAHTIKKFC
                     VVNRYDLSKEFPILTLRPTNLKAAIDELLWIWQKKSNNINDLNSRIWDSWADETGSIG
                     KAYGYQIGQKHKYKEGEFDQIDRVIYDLKYNPYSRRIITNIYNHNDLNEMNLYPCAYS
                     MTFNVTGNRLNAILNQRSNDILVANNWNVTQYAILVHMLAQISDLEVGELVHVIADAH
                     IYDRHIPLVKELINRESYDAPKLIINPNIKNFYDFRVEDFTLENYKSGPQIKKIPVAI
                     "
     gene            18565..19059
                     /locus_tag="KPH29_002170"
     CDS             18565..19059
                     /locus_tag="KPH29_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBG1467759.1"
                     /translation="MKVIVAVDLNWGIGYGGKLLQSIPEDMKFFKEKTVGNVVVMGRE
                     TFKSLPGKKALKDRVNIVLTRSKSFNDDRLIVCNSIDETLKELQKYENDKIFIIGGET
                     VYKQFLPYCNELYVTKIQKTYEADRFFPNIDIMSNWDLIKESEVKEYNNIRYVFSTYI
                     NNSL"
     gene            19086..20462
                     /locus_tag="KPH29_002171"
     CDS             19086..20462
                     /locus_tag="KPH29_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBG1467760.1"
                     /translation="MKKDIFVNRVSNALENDRGAVFVGSGISFSSTEVDWFKLLEPLV
                     NELDITLDNKSDDLPMIAQYIVNNYSGNRGPLVNEISKTFNRKFKLNKYHKYLATTKL
                     STIWTTNYDMLLEDAFYDFDTSVKVSDDSITRNRLEYDVEIIKMHGCISRSDWNEIVI
                     TQEDYDDFSINKPAISQRLSEDLINKSFLFIGYSYRDPNIRNIMINARRLSKKHTQQH
                     YIILKKLKDSDSNITQDDVVRQELWCNDLKRLGISTLFIDEYSELEELLREISQKSRG
                     KTVYVTGSHKESNMNARKLGTKLAKHKDITLLSGQSSGIGSEVVAAFTEECIKSKEDI
                     INRMKIFPNPYSADEKYSDDITLIPDLKKCRTKMLNSTQVVVVFSGGIGTEAEIDVAI
                     KQNCNIIPVITCKEDRENPAIKKILNYSDNMKNIEIKDATYYNKIVDEDGVVTMEEIY
                     ICISKIMN"
     gene            20438..21538
                     /locus_tag="KPH29_002172"
     CDS             20438..21538
                     /locus_tag="KPH29_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBG1467761.1"
                     /translation="MHIKNNELKHDIIKLKKRNGFIEVGKDKKTLVNCLIGANELEDY
                     KYEIKKIDCILNISEKPDIVSDLSTKRRKISESIWYKIVKDTPFVSATLPIYLVSKNN
                     NKIDSNELLDIIIEQMENGVGMITIHPTANKQIFEDSKNRMVPVTSRGGGMVIQDLIS
                     KNFSEDNVYLKILPEIIKYAQKHNVCISLGATFRSGNIFDSNDRAQITEIKSQIELAK
                     RIYKSNVGVIIESPGHSRPSDIIKISSILKKEGFPIMPLGPIPTDIAIGMDHIAAAIG
                     ATIMGIEGCANIISTVTREEHTGGRPSIESTVESIRTAKIAAHIIDIHQMNDINLDMS
                     VSKSRAKSNTCIFGKDTRYCDRCRELCPLCIR"
     gene            21581..22006
                     /locus_tag="KPH29_002173"
     CDS             21581..22006
                     /locus_tag="KPH29_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HBG1467762.1"
                     /translation="MHRIASFSQQSQRYVKLEQFEYIIPPEIEKIEKAKELFINSMKK
                     YQEDYDKLVEILFENHYNKLIKGGKNEKTSKRQVEKKAIEDARYVFPNACETKMVFTI
                     NARSLFNFFEHRCYENGSCLEGTMTCGDIVQVRKKFKNL"
     gene            22019..22756
                     /gene="rlmB"
                     /locus_tag="KPH29_002174"
     CDS             22019..22756
                     /gene="rlmB"
                     /locus_tag="KPH29_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBG1467763.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            22759..23286
                     /locus_tag="KPH29_002175"
     CDS             22759..23286
                     /locus_tag="KPH29_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBG1467764.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            23351..23998
                     /gene="sigH"
                     /locus_tag="KPH29_002176"
     CDS             23351..23998
                     /gene="sigH"
                     /locus_tag="KPH29_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HBG1467765.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            24072..>24463
                     /locus_tag="KPH29_002177"
     CDS             24072..>24463
                     /locus_tag="KPH29_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HBG1467766.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVCSATDGPMPQTREHILLSRQVGVP"
BASE COUNT        10138 a         2526 c         4531 g         7268 t
ORIGIN      
        1 attaggaaaa gtgatttttt tgtttttaaa aaaagtatta tttgtaaaat ataaaaaaaa
       61 atttcttaat atttgaataa aagaatagct taatttaaat gttattactt tataaaaaaa
      121 taataagtac aaaaaaaaca atatatggca tataagttgc tatataaata ataaggaggg
      181 aacaattata aatgaggcaa agaataaaaa acttaattga aaatgaagat aagaaaaatc
      241 ctttaacaga tgaagcgatt tcaagtaggt taaatattcg aagagaagat gttacctttt
      301 tgagaaatga gttaaaaata gaagattcaa gacaaagaag aaaagttgtt ttaattaaag
      361 ctataaaaga tattttaaaa gaagaaaaaa ctataaataa aaatgatata acaagaagat
      421 taaacagcat tgggtttaca gtatcaagat ttacagtcat acaatactta aaagagattc
      481 aagattctgg tgacctaaaa cttgataaag agagtgatga taaaaaagaa gaaataatca
      541 ataatagaga aaataaagat atagcatttg ataaactcat aggtagtagt ggtagcttaa
      601 gtacaattat aaaactagcc aaagcagcaa ttctttatcc acctcatggt cttcatacta
      661 ttattatggg gccaactggt gttggaaaga gtgagttagc agagtgtatg tataaatttg
      721 cagtagaatc atccagattc cctaagaatg caccatttat agtttttaat gcagcagatt
      781 attcagagaa tccacagctt ttgctatcac agttatttgg acacgttaaa ggtgctttta
      841 caggagctga tgagaacaaa gaaggattag tatcaaaggc agatggagga atattgttta
      901 tagatgaaat acatagatta cctcatgaag gtcaagaaat actatttcaa ctgattgata
      961 aaggtatggt cagaaagtta ggagaaacaa gactaacaca taaaatagat gtaatgatta
     1021 tttcagcaac ttctgagcca atagattctc atttacttaa tacatttaaa agacgtatac
     1081 ctgtaacaat tgaaatacca gaattagttg caagaccatt aaatgagagg tttgacatta
     1141 taaataattt cttcttaata gaagctcaaa gaatgggagc aaacatacat ataaagtcag
     1201 atgtattaaa agctcttatg ctttacgatt gtataggtaa tgtaggtcaa cttagaagtg
     1261 atattcaagt agcttgtgct aggggactat taaaccaact tacaaacaaa ctaaaagaag
     1321 taaatataag catatcagat gtacctggat atgtaaaaca aggtcttata aaaatcagaa
     1381 attgtagagg taaaatagaa aattatgtag atggggattt aattatagat tcaaatcttc
     1441 aaggtgagat agaaaagaaa aatgaagata tatatacttt tccagatgaa atatacaaac
     1501 ttactgaaag aagacatatt gatttattgg aacaaggttt agattttgat gttataaata
     1561 gaatcattgg aggagaatta gaaagttgca ttcaaaaata cataaaacaa gttaaaaaag
     1621 tttctattag taacaatctt ccagatataa gtccaattgt tggaaaatat atcgtagagc
     1681 taacttatga agtaatcaaa atagcatcac aatacctaag ttcactagaa ccgaatctac
     1741 atttatgttt agcagttcac ctaaaagcag cttttgagag attaaaagaa ggaaaagtaa
     1801 tatcaaatgc tcatttgaaa gaaataatag gaaattatcc actagaatat tctgtagcag
     1861 aaaaggtagc tcaatattta aaggaaagat ataatataaa cttaccaaaa gaagaaatag
     1921 gtataatagc aatatatttt aaaatgagta gtagaagaga tatgtctgaa caaaaaaaga
     1981 taggtgtact tcttatggct cacggaagag tagcatcttc tgtatgtgag gttacaaata
     2041 aactattagg aattaatcac gcaaggtatt tagatatgcc atttgagaag aagccggaag
     2101 aaatgattga agaagctact acaataataa aagatattga tgaaggtaga ggtgtgatta
     2161 tattagtaga tatgggttca ctttcatatt ttggagatat tatttctgga aagactggaa
     2221 ttaatataag gacaatatct agatttgata cattgctttc tatagaggtt atacgaagag
     2281 cagttttacc agatttaaca atagacgaga tagttgatga agttaaaggt atagagccac
     2341 aagtaaaaga aaaggttagt aaatgtaaca atacaaaagt agattttaga aaacctataa
     2401 ttttaacatt atgtattact ggattagggg gagcttgtaa gataaaaaaa atcctagagg
     2461 aaaatattaa aagtataact gaaaaggttg atatagaacc tataggtatg attgaatatc
     2521 caagtcttga tttagagata aaacaaataa gcaatgataa aaatgtaatc gccatagttg
     2581 gaacaattga ccctcagata gcaaatatcc ctttttttag tctagtagac ataaatgata
     2641 aagcaaagtt aaatcaaata aataacctaa ttgatacaaa tcaaattggt gaaacaaata
     2701 aaaaatatga attatcagat ttaatacata aagaagatat atttgtggat ttacaggtta
     2761 aatcaaaaga agaagttcta aaatatatga gtaaaaaact tagagagaaa ggttatgtga
     2821 caaaaaattt ctataaaaaa gtagtggaaa gagataactt atttccaact gaattatcag
     2881 atgaaatagc aatacctcac acagatggaa tagatgtaat taggccagct ataagtatag
     2941 ccatcttaaa gaagcctatt gtgtggagta aaagtaaagt aaaaataata cttttattag
     3001 ctgtagacca aaagtgtttt aatccattaa gcactctact ctcatttata gaaacagatg
     3061 aatttaagaa gataaaaaac attaaagata gagattttat tagggagatg atattagatg
     3121 gagtcaatga aaataattag tagcaattta atttttaaaa atattgaagt gtctaacaat
     3181 gaagatgcat taaagttttt gggacaacgt ttgtttgacg aacagtatgt aaaagaaagc
     3241 tacatacaag ctgttgtagc aagagaaaaa aagtatgcta caggattacc aacagaaata
     3301 tacggagtgg caataccaca tacagatata gtacatgtaa atgagccagg aatagcaatt
     3361 ggaatactga ataaaccagt aaaatttata atgatgggta cagacgatac tgaaattgat
     3421 gtaaaagttg tatttatgtt agcagtgaaa gagccacaag aacaattgca actattagaa
     3481 aaacttatga ctatatttca agataagaat atactaaata atattgttga cttaagtgaa
     3541 gaaagtgtga gtgatttatt aaactctaaa ttgaaaaatt aaacatgtaa agtatattaa
     3601 aaatgtaaaa ttaaacatgc aaagtatgtt aaaaaatata aaaagattat taaagatttc
     3661 taactataga tatctttaaa ataaaataat ttcaatgggg gtgactaatg tgactaaaaa
     3721 gatattagtt gcatgtggaa caggagtatg tacttcaaca attgctataa acaaattaaa
     3781 gaaagcactt caagacattg gtaaattgga tatggttaat attacacaat gtaaagtagt
     3841 agaagttgca tcaaaagcac ctgattatga tttaataatt tgtactacac aagtatctag
     3901 tagtataaaa acaccagtga taaatggatt acctttttta actggtgtcg gaatggataa
     3961 attaatcaat gatgtattag aagagttaga attataatta aaaaaattaa ggaacatggt
     4021 ctctaaaatc ataagtttta gagacttaaa attaaataat ggagggtctt tatatggaga
     4081 tattaaattt cattgttggt ctaggtgcac aagtaatgat gccaataata atatgtattt
     4141 ttggtttgat attaggtaca aaactaggaa aatctttaag agctggttta actgtgggag
     4201 ttgggtttat aggtcttaac actattatag ctttgctaac agataattta ggtccagcta
     4261 cacaacaaat ggttaagaat ttagggctaa gtttatcaat aatagatgta ggatggccag
     4321 cagcatcagc tatagctttt gcatcaacag taggggcttt aattattcca ataggtcttg
     4381 tggtaaatat agttatgcta attactaata ctactcaaac tgttgatgta gacatatggg
     4441 attactggca ttttgctttt acaggagctt tagtggcagg ggctactcaa agtgtaatgt
     4501 ggggagtatt tgcagcagta gcaaatatgg taattgtatt ggtaatggca gatttaactg
     4561 ctccaggtat agaggaatat ttaggtatgc caggtatatc tcttcctcat ggatttacac
     4621 aggcatttgt tccaattgct atagtagtaa ataaattatt agacctaata ccaggtataa
     4681 ataagattga gataaatgct gatacattgc agaaaaaatt tggattattt ggagaacctt
     4741 taataatggg gtctgtaata ggtgtaataa ttggtattgc tgcgaaatat gatataaaag
     4801 gaatattaca attaggtgta actatgggag ctgtattaat attaattcca aagatggcag
     4861 cattattaat ggaaggatta ttaccagtat cagaagctgc acaggaattt attgagaaga
     4921 gatttaaaaa cagaggcaag atttatatag gcttagattc agcagtagga ataggtcatc
     4981 ctgttacatt atcagttgca ctagtgttgg ttcctttgac tatacttata gcagctatat
     5041 tgccaggaaa taaagtttta ccatttgcag atttagcagt aattccattt gcacttgtat
     5101 taatcgtacc aattacaaag ggaaatgtgt ttagaacatt aataatagga ataattataa
     5161 ttacttctgg attattaatc gcaactaact tagctccatt atttacccaa atggctttaa
     5221 atgctagctt taaaatgcct gaaggagcaa ctatgatttc aagtatttgt gatggtgcga
     5281 atccattaag ttgggtattt gtaaaagtta tgaactataa agttattgga ggagtagttt
     5341 ttggagttat agctttagga atggctattt acaataggaa tagaataata aatgaaaata
     5401 aaaagctatc tatagaagaa taaggttaaa acaattttat taaggagtgt ataggttatg
     5461 aaaaaatata aatatgattc taaaaggtac ttactcagtg ttacaatacc aggatttttt
     5521 atgattttaa tattgattta tgcactgttt aataactata taaattttaa tttgaatata
     5581 tatagtttgt taataattgt ctgtacttat ggtatattca atacttttat atcatcaagt
     5641 aatcctaaaa aaattattat tgacagcaaa tgtatacatt ttacctcatt tatgactaca
     5701 cataagtatg aaattaaaga tatagaaaag ataagaataa gagaatttta taataaaaaa
     5761 atttatctaa aaattaatga tggagacctt tttaaaggta ggtattggat aagaacaaat
     5821 atgtttaata atagtatagc attatatagt tatttcattg agttggaaga gtgcttatat
     5881 ccagattcac taagatttag aaataaaaaa tttgagaata agaatatata aaaataaaaa
     5941 attgaattga agcctaactt aatctttttt gataagatat aagtttgaac tttatactaa
     6001 atttttagaa ttttcaatca agacaataac ataatagcta agcaattaga acacaactaa
     6061 gatgtataag gcatgtatat gatgaatggg aggatttaat taatgttatt agagaaatta
     6121 agaaaagaag tattacaagc atctttagac cttttaaact ataatttagt gacattaaca
     6181 ggagggaatg taagtgggag agatgaacaa actggctata tagcaatcac cccaagtgga
     6241 atggattata gaaatcttac accaagtgat attgttatag tagatgtaga tggaaatatt
     6301 atagatggaa aatggaaagc atcagtggat ttatcagacc acttgtatat atataagcat
     6361 agagaagaca taaatagtat aattcataca cattctacat actcaagttg ttttgctatt
     6421 ttaaatgaac ctatagaatg cgcaagtaca actcttgcaa atgaagtagg gggttcagta
     6481 ccagttgcaa aattttcacc accaacattt aaaaagatgg gaaaatgtgt tattgaagca
     6541 attggtgaca aaagagcatg tcttttagca aatcatggcg taattgcagt tggtccatct
     6601 gtaggacatg ctcttacagc cgctgtaatg ttagaagatt cagcaaaagt atattactta
     6661 gcaaaatcaa ttggcacacc agtgctactg cctgatgaag aaattcaaag agcgagagat
     6721 gtatttttta atgtctatgg tcaggataaa taaattagat aaaattttaa aagtaatatg
     6781 aaaatataga tattcaatga tattaaggag atttataatg atataataag tttaatagtt
     6841 aagaagtaaa atatgaaatg taatagtata taaattcatt taagactaaa aataataaga
     6901 taaaataaaa aggagtctgt atttctatgt attacttatt aaacaaaagc acaaatcctt
     6961 actttaattt ggcattagaa gaatatctat ttttaaatga taaatataat gatgatatta
     7021 taatcatatg gagaaatgaa gactccattt ttataggtaa aaatcaaaat ccatatcagg
     7081 aagtacatca tgatgttatt gaaaagggag aaattcctat tttaagaaga atatctggag
     7141 ggggcactgt ttatcatgat ttaggtaata tcaatatgtc attcatacaa aaagataggc
     7201 aacttcatga gatagatttt ttggagcata ctaagtttat gcaaaagatg ctttcaacct
     7261 taggactaga tgtatctata actgaaagaa aagatttatt tttaaatggg aaaaaaattt
     7321 caggctctgc acagtctata aaaagaaaaa attctcttta tcatggaact cttctttacg
     7381 attcagattt aaataaatta actaaatatt taaattcaaa taaagcaact gaatcaaatg
     7441 caactaaatc tgtttcaagt aaagttacaa atattaagtt tttactcgaa aaagatataa
     7501 attacttttt agattattgt gttgaatatt taaaatctaa tataaagaat ataaaagaac
     7561 ttgaattgga tgaagaagat attaaaagtg tatacaatat aggagaagat aaatatagaa
     7621 agatagagtg gacatatggt aaaacgccga aatttcaagt agtgctacca tttaataatg
     7681 aaagtaaaat aaatatacat gttaacagat ggaaagtatc aaaatttttt atttcttatg
     7741 atgataatgt aattaatatg gataagttgt tagatttgaa tttctttaaa gaagaaataa
     7801 aagagagtat tttagaaaat tatccacatt acatagattt aatcaattta atattttaat
     7861 ataaaacctc taatttgata aaattgtctt atatcaaact agaggttttt aatattcttg
     7921 catattaaca accattacaa aattgtaaaa ttaaaaacaa cactattgaa aaatcaatat
     7981 atattgtaat aaatatatgt gtttttcatt agcaaataga cattaactat ttatacaact
     8041 gaaaatttaa tatataatta atcagatggt taaaataaaa atattaaaag ttttaataaa
     8101 tgatggttaa aaataaaata cgaggtgata ccatgtacag tgttataatt gttgcagcgg
     8161 gaagcggaag gagaatgaac ttagatataa acaaacagtt cataaaatta agagaaaaag
     8221 aaattattgc tcatacaatt caagtttttt atgaaaatat aaatatagat gaaatagtgg
     8281 tatgtataaa aaaagaagaa gaggattttt ttaaagaaaa tataataaat aagtataact
     8341 ttaaaaatat aaaaatagca tatggcggaa aagaaagaca ggactcaata tataatggtt
     8401 taaaaaaatt agatgaaaat tgtgatatag tacttatcca tgatggagca agaccatttg
     8461 tcgaccatag aataatcaac gaatctataa aggtagctaa agaaaaaaag gctgtggttg
     8521 taggtgttcc agtaagtgat actataaaga tagtgtcaga tggaacagta caagaaactc
     8581 cagaaagaaa tttgttgtgg gcagcacaaa cccctcaaac ctttgaatat aatctcataa
     8641 tagatgctta tgagcaagca tataagaaca attattatgg tactgatgat tcaatgttgg
     8701 tagaaaatat aggtcaaagt gtcactatgg taatgggttc ttatgaaaac ataaaaataa
     8761 ctagtccaga agatttaaat atagcagaac aaatattaaa tatggaaaaa agagatgaag
     8821 ttaacagtag aaggagaatt atatgagaat aggattagga tatgatgtac acaaattaac
     8881 agagaataga aaacttataa tcggtggtgt agagatacca cacgataaag gtttgttggg
     8941 tcattctgat gctgatgtat tgatacatgc aataatggat tctattttag gtgctctagc
     9001 attaggagat attgggaagc atttcccaga tacagatgaa gaatataagg gagcagatag
     9061 tatgaaatta ttagaacatg tgtataatct aataacaagt aaaggatata aaattggtaa
     9121 tatagatagt actataattg ctcaaagccc taagatggct ccatatattg agagtatgag
     9181 aagtaatata tcaaaagttt taaacacaga cattgataat ataaacataa aggcaacaac
     9241 agaagaaggt cttggtttta caggagcaaa acagggaata gcttcacaaa gtatttgttt
     9301 attattgttg acaagtcaga ataattaata tattattagt aataagttta atggatatca
     9361 agtaataggc ctatatagaa tatctataat gtatgaattt aagtgaaatt aatataaata
     9421 taaaattagc tatgataaag aaatagtaaa atgatgaaat cttcacagag agaaaacggt
     9481 ggtgagagtt ttcaagagga agtctttgaa cctgtctttt agcttaaagg tataaaaatc
     9541 tttacggtag gaaccgttat attcccaaaa gagagctatt catatagcta actagagtgg
     9601 taccgcggaa attaaacctt tcgtctctat acttgtatag agatgagagg ttttttatat
     9661 tttcaggaag ttaatattca ggaggaatct attaatgaaa atgtcaaaaa tgtttatgcc
     9721 gacattaaaa gaaatcccag cagatgctga gattacaagc catcaattaa tggttaggtc
     9781 aggcatgata aaaaaaatga cttcaggtgt ttataaccaa ttaccaatgg ggttaagagt
     9841 gttcaaaaaa attgaacaaa taattagaga agaattaaat aaaaaagatt gtcaagaaat
     9901 actttgtgca gccttacttc cttctgaatt atggaaagag tctggaaggt ggactgctat
     9961 gggtgaagaa atgtttagac taaaagacag aacagaaaga gagtactgtc ttgggccaac
    10021 acatgaagaa gcattcactg atataataag acaagaaatt acttcatata aacaattgcc
    10081 actgaacttg taccaaatac aagtaaaata tagagacgag agaagaccaa gatttggtgt
    10141 tatgagaact aagactttta caatgaagga tgcatatagt tttgatgtag atgataaagg
    10201 tttagataag tcatatcaag atatgtttga tgcatatgta agtatatttg atagatgtgg
    10261 attggagaat agccctgtac aggcagattc aggagctata ggaggttcta catcagccga
    10321 atttatggtt aagtcagaag ttggtgaaga tgaagttgta ttttgtagtg gatgtgatta
    10381 tgcagccaat gtagaaagag cagaatcttg taatctagca tcacaaaaag aagaaatgaa
    10441 agaattagaa gaggttcata caccaggagc agctacaata aaagagttag aagaattttt
    10501 aaaaacttct ccagataagt ttgctaaaac tttagtatat gaggcagatg gaaaaacagt
    10561 tgtagtcgtt gtaagaggag atagagaagt taatgaaatc aaagtatcta atgctatagg
    10621 ttctgttata gaatttgctc ttgctacaga tgatgtagtg agaaaagtaa ctaatgcaga
    10681 agtaggattt gcaggtccaa taggtataaa tgctgactat gtatttatag ataaagaaat
    10741 tgttgaacaa agaaacatag ttgttggggc taataaaact gagtaccata taaaaaatgc
    10801 taactatgga agagattttg aaggaatagt tggagatttt agaaatgttc aagaaggcga
    10861 ccagtgtata gtatgtggta agccacttga aatagctaga ggtgttgaag taggacatat
    10921 atttaaacta ggaactaagt actcagaatc tatgaatgca aacttcatag acaaagatgg
    10981 aaaatcaaag cctatagtaa tgggatgtta tggaataggt gttgaaagaa cagcagcagc
    11041 tataattgaa caacataatg atgagaaagg tataatatgg cctttgtcag tagctccata
    11101 ccatgttgtg atagttccag caaatatgaa aaatgaagaa caaatatcaa tagctgaaaa
    11161 tatatataat gatttgcaag ctatgggtgt agaagtttta ttagatgaca gagatgaaag
    11221 aataggtgtc aaattcaatg attcagaatt aataggaata cctatgagga ttacagtagg
    11281 taaaaatatt aatgaaggta aagtagaatt taaacttaga cataaagaag ataaagagat
    11341 aattgacata gaagaaataa atgaaaaagt aaaggcagag tttatcagga ataatgttag
    11401 gttaggataa taacttaaat aagatattgt atagataatc aaagttgtac tttatagaca
    11461 ccattttatc tatatataag gatataaaat ggtgtttttt tataaaacaa ataaatgagg
    11521 aggtaattga aatggcaaaa aatgaaaaac aatttgttga agaaataaca aagatggaag
    11581 atgattttcc acaatggtac acagatgtaa taactaagac tgacttagtt gattatgcac
    11641 cagtaaaagg ttttatggtt gtaaagccat atggatatgc attatgggaa aagatgcaag
    11701 aatttatgga taaaaaattt aaagaaactg gacataaaaa ctgttatttt ccattattaa
    11761 taccagagag tttattaaac aaagaagcag aacacgttga aggttttgct ccagaagttg
    11821 catgggtaac acatggagga aataaaaaat tagaagaaag attatgtgta agacctactt
    11881 cagagactat aatatgtaca atgtatgcga aatggttaaa atcatataga gaattaccat
    11941 atctttataa tcaatggtgc tcagttgtta gatgggaaaa atcaactaga cctttcttaa
    12001 gaacatctga gtttttatgg caagaaggtc atacattaca tgaaacagca gaagaagcac
    12061 aagaagaaac aatacaacaa ttagaagtat ataaagcgtt atgtgaagaa ttacttgcaa
    12121 tgccagttgt agctggtcaa aaaagtgaaa gtgagaaatt tgcaggtggt gaaagaacat
    12181 atactataga agctatgatg catgatggta aagcattaca atcagggaca agtcacttcc
    12241 taggtcaaca ctttactaag gcttttgata ttacttttgc agatagagaa ggaaatttag
    12301 ctaatccata ccatacatct tggggagcat ctacaagact tataggtgga cttataatga
    12361 ctcatagtga taacagaggt ttagtattac caccaagagt agctccaatt caagttgtta
    12421 tagttcctat agctgctaaa aaaggaaatg taatggagac tgttgataaa atatatgctg
    12481 atttaaaaac taagggagta gccgtagaag ttgatgatag agataactat acaactggat
    12541 ggaaatttaa tgagtgggaa atgaaaggtg ctccagtaag agttgaaatt ggaccaaaag
    12601 atatagaaaa taatcaagct atggtattta gaagagatac tttagaaaag gattctatgc
    12661 ctttagaagg attagctgat gctatatgtg atttatttga tgttatacac aatgatatgt
    12721 ttgaaaaagc aagaaaacat agagaagaca atacatctat agttgaaaat atggatgagt
    12781 ttagaaaagc tttagaagaa aagccaggat tcataaagac tatgtggtgt ggagatgcag
    12841 aatgtgaagc taagattaag gaagaaacag gagcaactat aagatgctta ccttttgaac
    12901 aagaaaactt aggtcacaag tgtgtgtact gtggaaaaga agcagatagt atggtagtaa
    12961 tggcaaaagc ttactaaaat acatttaaat aaaaactggg gtagaaagta tttaatactt
    13021 tatcccagtt tttttgtttt ttatgagtaa taaaatgtat ttgtaactat gtcataagat
    13081 aagtcataga tacttatatt aaaaagaata taatagagaa ctatttttaa taattacata
    13141 ttaatgaaag caaaatattt ttgttatata gattttcaat aaagcaaaca ataaatataa
    13201 atgaatatta gtaaggagtg cttttaattt ttagaaaata aatgacttaa aagaaagatt
    13261 tatgaaatta aaactaggat tttctgatat aataaacatg tgtattttta aataaaaagg
    13321 atattattat tagatttgat taaaaataaa cttgcaaaac aatgtactag tttaatatta
    13381 tagttatatt gaataaaata tcaggaggaa gtaaaaatga atgtaagagt aaggtttgca
    13441 ccaagtccaa caggatttgt acatataggt agtttgagaa ctgctttata taactattta
    13501 tttgcaaaaa aaatgggtgg agaatatata ttaagagtgg aagatacaga ccaaagtaga
    13561 ttagtagaag gtgctataga gaatatgctt aatgctatga aatgggctgg tgtcaaccat
    13621 agtgaaggtg ttatattaga tgatagtgga aaagtagtac aaaaaggaga atatggtcca
    13681 tatattcaat ctcaaagatt agatatatac caagaatata taaagcaact tttagatagt
    13741 ggaaaagctt attattgttt ctgtacaaaa gaaagactgg atgaagtaag agatgctcaa
    13801 agagcagcag gagaaacagc taaatatgat ggtcattgta agaatctttc taaagaagaa
    13861 gtagaagcga atataaaagc aggaatacca tatgttataa gattaagatt gccagagaat
    13921 catacaatta agtttacaga cttagttaga ggagatatgg aatttaatac taatgaccta
    13981 gatgaccaag tattaatgaa aacagatgga tttccaactt atcattttgc agtagttgtt
    14041 gatgactatt tgatgaaaat tacacatgtt ataagaggag aagaatgggt atcatctact
    14101 ccaaaacatg tttatttata tgaagctttt ggatgggaag cacctgtatt tgtgcattta
    14161 ccaaatatac ttaataaaga aaagaaaaaa ttaagtaaaa gacaaggtga tgtagctgtt
    14221 gaagacttta agaaaaaagg atatttacca gagggattag taaactatgt agcattagtt
    14281 ggttggtctc ctgaagataa tcaagaattg tttacaatgg aggaattaga aaaagccttc
    14341 tctgtagaaa gagtatctaa aagtggtgga gtatttgata ctgaaaaatt aaattgggtt
    14401 aatcagcact atataaaaga tggtgatgat gcttatctaa cagacttagc aataccattt
    14461 ttagtagaag atggatttat aacagaagaa gaagctacta ataaatatga tttcttaaag
    14521 agtatgatat cagtacttaa agagaaatta caatatgtaa aagaaattac tgaacatgca
    14581 agtatatttt ttggagataa aatagaggta gaaactgaag agggaagaga gttcttaaga
    14641 ttagaacata tacctacttt aatagatgct ttgagagaaa aaatagaaaa aacagaggta
    14701 ttaaatgctg agtttgttca agctatgctt aaagaaatac aaaaagagta caaaataaaa
    14761 gggaaaaatt tatttatggg ctcaagaata attttgacag ggcaaatgca tggtccagat
    14821 ttaccaaaag taatggaagt tttaggaaaa gaaacttgct taaatagaat agcttatgta
    14881 aaaaataata tattatagtc ataaaagtcc gctaatttag aagcggactt tttttgtgtt
    14941 agtatttggt gaaaatatct gcttaagaaa atgaaggtat ttaaggtaat aaatttaata
    15001 tataaaaagc aaagcaaaaa tataaataaa atatgaataa taaactaata aaatattaag
    15061 catagaagaa gggaatctat tattttagtt tgatttaatt attaaggggg taattactat
    15121 gtatagtaat attgatgatg ttaaaaagga attaaaggag ctatgcttag aatatgttac
    15181 aattttagaa aaattaaaag atgaaaaaat gattacagaa gaaacttttg aaaagtgctc
    15241 ttctcaaaaa aaaatatttt tagaagaaca atagaggatg gtaatagtat tattattatg
    15301 tagtttgaaa gatagtacga aaaatgatag taatactatt atttattgca attttcaact
    15361 ataatataat atataatgtt aagaagataa tttataaaaa tacagtgagg tgatgagtgt
    15421 gaaagtttat aatacattga ctagaacaaa agaagaattt gttccattag aagaaggaaa
    15481 ggtaaaaatg tatgtgtgtg gtccaacagt ttataactat atacacatag gtaatgcaag
    15541 accatttata atatttgata ctctaagaag gtatttagag tatagaggat acgatgtaac
    15601 ttatgtacaa aactttacag atgttgatga taagattata aatagaagtc atgaggaggg
    15661 aatatctcct gaagaagttg cggctaagta tataaaagaa tattttgtag actgtgatgg
    15721 gctagggata aaaagagcta ctgttcatcc tcaggttaca gataatatac aacagattat
    15781 agagtttata aaagagttgg aagataaggg atatgcatat gcagtaaatg gtgatgtata
    15841 ttttgataca aataaatttg aaggttatgg aaaactttca ggtcaaaaac aagaagattt
    15901 agaggcaggg gctagaattg aggttaatga ccaaaaaaga catcctatgg actttgttct
    15961 ttggaaggca aaaaaagaag gtgagccagg atgggatagc ccttggggag aaggtagacc
    16021 aggatggcat atagaatgtt ctgttatgtc taagagatat ttaggtgaaa caattgatat
    16081 acatgcaggt gggcaagatt taacattccc acatcatgaa aatgaaatag ctcaaagtga
    16141 agctagaagt ggaaaaatat tctcaaaata ctggatgcat aatggatata taaatataaa
    16201 tgatgaaaag atgagtaaat caaaaggtaa tttctttaca gtaagagata tatcaaaatt
    16261 atatgattta gagatagtaa gatttttcat gttatcagca cattatagaa atccagtaaa
    16321 ctttagtgat gagatgttaa atcaagctaa agcaggtctt gaaagactat ataatactaa
    16381 agaaaaatta gaatttacgc taagtaattt agtagaatct cctttaacag aaaaagaagt
    16441 agagcttgtt aaagaattag atgactttag acaaaaattc atagatgcta tggatgatga
    16501 tgttaataca gcagacgcag taagtgtaat atttgaatta gctaagttaa taaattctaa
    16561 tgtagatgaa aattcttctt tggagtttgc taaaaaatgc ttagatgaat ttaatgaact
    16621 tacaggagta ttaaatattg taaataagaa aaaggatact gtattagata aagacattga
    16681 agaattgata caaaaaagaa ctgatgcaaa gaaaaataaa gagtttcaat tagctgatga
    16741 tattagacaa caattactag atatgggaat tgtattagaa gacacaagac aaggtgttaa
    16801 gtggaaaagg atataaaaat ggaaaaaact gaattagtta ctatgtcacc attagtttta
    16861 gcatacttag gtgatacagt gtatgagaca tatataagag aatatttaat tagacagaat
    16921 acacaaagaa aagttaatga tttgcataaa ctagctataa agtatgtaaa agctaaagct
    16981 caggcaacta taattcatga aattgaaatt gagttaactg aagaagagag taaaatttat
    17041 aaaaggggaa gaaatcaaaa gtcaaatact tctcctaaga atgcagatat tatagactac
    17101 aaacatgcaa ctgggtttga ggcattagtg ggatatttgt atttaaataa tgaaatagag
    17161 agacttcaat atattatcaa taaaggaatc aaaattattg aaagagatat gtaaatatta
    17221 aagagtgttg tcttaataat aagtaataag gcaacacttt ttttataact aatgtataat
    17281 aaatatatag aaattagtaa tgagaaagga taagaaagtt atgaaagtaa aattaatatc
    17341 acatacacct gaaccagaaa aagtaatagc tatggcagct aagctatgtt attcaccagt
    17401 tgggacagac gaaatagaaa aagatttcta aaatatatta aagagcattc ctataacaaa
    17461 tactatggcg gttggaatct atgagaagtt atagatatag aattatgtaa attatttgga
    17521 aaaaacaggt ttgaaaatta gccatgttgg tatttctagg ttacttaagg aagatcaaac
    17581 ataaataagc tgagttgtct taggtgcact atgtacaaca ttagaatgtg aagttgaaga
    17641 tttattactt caaacgaacg atggtactaa gaaaaacaat gatgaaatga ttaattaata
    17701 ataaaggtaa ggaggtagta tatgagttta gcagataaaa tttttattga tatgtgtaat
    17761 aatattcttg aaaatggagt atcatcagat ggagaggtag ttagagcaaa atgggatgat
    17821 ggccagcctg ctcatacaat aaagaagttt tgtgtagtaa ataggtatga tctttcaaaa
    17881 gaattcccaa tacttacact aagaccaaca aacttaaaag cagcaataga tgaattactt
    17941 tggatatggc aaaaaaaatc taataatata aatgatttga atagccgtat ttgggatagc
    18001 tgggctgatg aaacaggttc aatagggaaa gcttatggat accaaattgg acagaaacat
    18061 aaatacaaag aaggagaatt tgaccaaata gatagagtga tttatgactt gaaatataat
    18121 ccttatagta ggagaattat aactaatata tataatcata atgatttaaa tgaaatgaat
    18181 ttatatcctt gtgcatatag tatgacattt aatgtaactg gaaatagatt gaatgcaata
    18241 ttaaatcaaa gatcaaatga tattttggta gctaataatt ggaatgtaac tcagtatgca
    18301 attttagttc acatgttagc acaaataagt gatttggaag taggcgaatt ggttcatgtt
    18361 atagcggatg cacatatata tgatagacat attcctctag ttaaagagct tattaataga
    18421 gaatcttatg atgcaccaaa attaataata aatccaaaca taaagaattt ctatgacttt
    18481 agagttgagg actttacatt agaaaattat aaaagtgggc cacaaatcaa aaagattcct
    18541 gtggcaattt agaaggagtt atttatgaaa gttatagtag ctgtagattt aaattgggga
    18601 attggttatg gtggaaagtt actacagtcg attcctgaag atatgaaatt ttttaaagaa
    18661 aaaactgtag gtaatgtagt tgtaatgggg agagaaacct ttaaatcatt accaggaaaa
    18721 aaagcattaa aagatagagt gaatatagtc ttgactagaa gtaaatcttt taatgatgat
    18781 agattgattg tttgtaattc tatagatgaa actcttaagg aactacaaaa atatgaaaat
    18841 gataaaattt ttataatagg tggagaaaca gtatataaac aatttttacc ttattgtaat
    18901 gaactatatg taacaaaaat acagaagaca tatgaggcgg atagattttt ccctaatata
    18961 gatattatgt caaactggga tttgattaaa gaaagtgaag taaaagaata taacaatata
    19021 agatatgttt tttcaacata tataaataat agtttatagg tttgtatagg agagaaattt
    19081 aatttatgaa aaaggatata tttgtaaata gagtttctaa tgctttagaa aatgatagag
    19141 gcgcagtttt tgtaggtagt ggtatatcgt ttagttcgac tgaagttgat tggtttaaat
    19201 tactggagcc attagtaaat gaacttgata taacattaga caataaaagt gatgatttac
    19261 ctatgattgc tcagtacata gtaaataatt attcaggaaa tagagggcct ttagtaaacg
    19321 aaatatcaaa aacatttaat agaaagttta aactaaacaa gtatcataag tatttagcaa
    19381 caactaaact ttcaacaata tggacaacta attatgatat gttgttagaa gatgcatttt
    19441 atgattttga tacatcggtt aaagtaagtg atgattctat tacaaggaat agactagaat
    19501 atgatgtgga aattataaaa atgcatggat gtatttctag atccgattgg aatgaaatag
    19561 ttataactca agaagattat gatgattttt caataaataa accagctata tctcaaagat
    19621 taagtgaaga tttaataaat aagtcttttc tatttattgg atatagctat agagatccaa
    19681 atataagaaa tataatgata aatgctagaa ggctaagtaa aaaacatact caacaacatt
    19741 atataatttt aaaaaagtta aaagatagtg atagtaatat tacgcaagat gatgtagtaa
    19801 gacaagaact ttggtgcaat gatttaaaac gtttgggaat ttcaacttta tttatagatg
    19861 aatactcaga gttagaggaa cttttaagag agataagtca aaaatcaaga ggtaaaacag
    19921 tatatgtgac aggttctcat aaagaaagca atatgaatgc aagaaaatta ggaaccaagc
    19981 tagcaaaaca taaagatatt actttactaa gtggtcaatc atctggtatt ggctcagagg
    20041 tagttgctgc gtttactgaa gagtgtatta agagtaaaga agatattata aatagaatga
    20101 aaatatttcc taatccatat tcagctgatg aaaaatactc tgatgatatt actttaatac
    20161 cagatttaaa gaaatgtaga actaaaatgt tgaattcaac acaagttgta gttgtattta
    20221 gtggaggtat aggtactgaa gcagagatag atgttgctat aaaacaaaac tgtaatataa
    20281 ttcctgttat aacatgtaaa gaggatagag agaatccagc tataaaaaaa atcttaaact
    20341 atagtgataa tatgaaaaat atagaaataa aagatgctac ctattataat aaaatagtag
    20401 atgaagatgg tgttgtaaca atggaggaaa tttatatatg catatcaaaa ataatgaatt
    20461 aaaacatgac attattaaat tgaaaaaaag aaatggattt attgaagttg gaaaagataa
    20521 aaaaacgttg gtaaactgtt taattggagc gaatgaatta gaggattaca aatatgaaat
    20581 aaaaaaaata gattgtatac ttaatatatc agaaaaacca gatatagttt cagatttaag
    20641 tactaaaaga agaaaaataa gtgaatctat atggtataag atagttaaag atacaccttt
    20701 cgtatctgct acactgccca tatacttagt tagtaaaaat aataataaaa tagatagtaa
    20761 tgaattatta gatataatta ttgagcagat ggaaaatgga gttgggatga taacgattca
    20821 tccaacagca aataaacaaa tttttgagga ttctaagaat agaatggtac ctgtcacatc
    20881 tagaggtgga ggtatggtaa tacaagattt aatctcaaaa aatttttcag aagataatgt
    20941 gtatttgaaa atattgccag aaattataaa gtatgctcaa aaacataatg tttgtatcag
    21001 tctaggtgca acatttagat caggaaacat atttgattct aatgatagag cacagataac
    21061 agaaattaag agtcaaatag aattagctaa gaggatatat aaaagtaatg tcggggttat
    21121 tattgaatca ccaggtcatt caagaccaag tgacataatt aaaatatcgt caatattaaa
    21181 aaaagaaggt ttcccaatta tgccactagg accaatacca acagatattg caataggaat
    21241 ggatcatatc gctgcagcta ttggtgctac aattatgggt atagaaggat gtgcaaatat
    21301 aatatcaact gtcacacgag aagaacatac tggagggaga ccttctattg aatccacagt
    21361 tgaatctata agaacagcaa aaatagctgc tcatattatt gatatacatc aaatgaatga
    21421 tattaactta gatatgagtg tatctaagtc tagagctaaa tctaacacct gtatatttgg
    21481 gaaagataca agatattgtg acagatgtag agaactgtgt cctttgtgca ttagataaaa
    21541 attatattca agttaattca ataaaaaaga ttggataaaa atacatcgta tagctagttt
    21601 ttcacaacag agtcaaagat atgtcaaatt agagcaattt gaatacataa ttcctccaga
    21661 aatagaaaaa atagaaaaag caaaagagtt atttataaat tcaatgaaaa aataccaaga
    21721 ggattatgat aaattagtag agatactatt tgaaaatcat tataacaaac taataaaagg
    21781 tggtaaaaac gagaaaacat caaaaagaca agttgagaag aaagcaatag aagatgctag
    21841 atacgtattt ccaaatgcgt gtgaaacaaa gatggtattt actataaatg ctagaagttt
    21901 gtttaatttc tttgaacata gatgttatga aaatgggagt tgtctagaag gaactatgac
    21961 ttgtggagat atagttcaag ttagaaaaaa atttaagaac ttgtaggagg tgaataattt
    22021 ggcgagtata gaaggaagaa atccggtaat agaagcaata aaaagtgaca gagaaataga
    22081 taaaatatta attgcaaatt cagctaaaga aggttcaatt aagaaaataa taggaatggc
    22141 taaagataag aatataatta ttcagtatgt tgataaacat aagctagatg aagtaagtac
    22201 aagtcattca catcaaggtg taatagctta tgcaagtgag tataagtatt atgaattaga
    22261 tgagctaata gatttagcaa agaataaaga tgaagaccca tttttcatta tattagatga
    22321 gataacagac ccacataatt tgggttcaat aataaggaca gcagatgcag taggagcaca
    22381 tggagttatc attccaaaaa gaaggtctgt acacataact cctgtagttg ccaaagcatc
    22441 tgctggagca gtagaatata tgccagtttg caaggtaacc aatatagtta atacaataaa
    22501 aagactcaaa gaagaagggc tgtggattgc tgcagctgat atggatggag aaacttttta
    22561 taaacaaaat cttactggtc ctcttggtgt agtaattgga agtgaaggtt ttggaatatc
    22621 tagattggtt aaacaaaact gtgactttat agtaaaaatg ccaatgatag gtaatgttac
    22681 atctttaaat gcatctgtag caggtggaat acttttatat gagatattca gacaaagact
    22741 ggataaaagt aaataggcat gaaaaaaaat ataaatcatt acttaataat agatggctac
    22801 aatattataa atgcgtggga caatttgaaa gaactggcta aagaggactt agaagattct
    22861 cgagaaaagc ttattgatga cattatagaa ttttctgagt ttatgggtta taagactata
    22921 atagtttttg atgcttataa tgttaaaaat tctcgagaaa aagttgaaaa aagaaaacac
    22981 ataacaatag tttatacaag agaacaccaa acagcagata gttatataga gaaatttata
    23041 acttctttat caaagtatga tgatgtaaaa gtagccacta atgactatgc agaacagcaa
    23101 atgattttag gaaaaggtgc tactagaatg tctgcaagag aattaaagtt agagttagat
    23161 cgttccaaga ataaaatgaa ggaaaaaaat atcggtttga ggaaaaaaat tcagcgaaat
    23221 tggttggagg aaaggttaga taaagaaaca ttgtcgaaac ttgagaacat tcgtagaaag
    23281 cgttgaaatt actaggttct ttagactata atatacctat aaatttgttt tatatatggg
    23341 ggagatttac atgttggtag caaaagaaaa aagttatgag ttagtggaca attgtcagca
    23401 ggatgagtat aacattgtat taagagcaag tgaaggggat aaaatagcac tagagtacat
    23461 tattactaaa tacagaaatt ttgtaaaggc aaaagcaaag tcatattttt taattggagc
    23521 agataaagaa gatataatac aagaaggaat gataggcctt tataaggcag ttagagattt
    23581 tgatggaagt aagaccaatt catttaaatg ttttgcggag atatgtataa caagacaaat
    23641 aataactgct ataaaaactg caacaagaca aaaacatata cctttaaatt catatgtttc
    23701 tttgaataaa ccaatatatg atgaagagtc agacagaaca cttttagata ttatagctac
    23761 aagtatagta acagatccag aagagcttat aattagtaaa gaagagttaa aaaatataga
    23821 atcaaaaatg aacgaattgt taagtgactt ggaattagaa gtactagaat tatatttaaa
    23881 tggaaagtca taccagttta tagcggacaa gcttaaaaga gatgtaaagt caatagataa
    23941 cgctttacaa agggttaaga gaaaattaga aaaacatctt gaaaacagaa atgattaata
    24001 gtaaaatact taccataagc ttattgtgag caaaattaat ttatatcgaa agataaggga
    24061 ggaaattcaa aatggctaaa gctaaatacg aaagaacaaa acctcatgtt aatataggga
    24121 caataggaca cgtagaccac ggtaaaacta cattaacagc agcaataaca aaaacattat
    24181 atgacagata tcaattagga gaagcagtag atttcgcaaa catagataaa gctccagaag
    24241 aaagagaaag aggaatcaca atatcaacag cacacgttga gtatgaaaca ccaaatagac
    24301 actatgcaca cgttgactgc ccaggacatg ctgactacgt taagaacatg ataacaggag
    24361 cagcacaaat ggacggagca atattagttt gttcagcaac agatggacca atgccacaaa
    24421 caagagagca tatactatta tcaagacaag ttggagtacc ata
//
LOCUS       DAELXX010000059        24141 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.059, whole genome shotgun sequence.
ACCESSION   DAELXX010000059 DAELXX010000000
VERSION     DAELXX010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24141
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.059"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..265)
                     /locus_tag="KPH29_002178"
     CDS             complement(<1..265)
                     /locus_tag="KPH29_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467767.1"
                     /translation="MLVRVKIVIGATIRYLIKHKVNYCNKKSLDKLELKYGNIDREKA
                     VKLEIFYQYLIELEYIEMSLLIRRLDTAITSLALVSIITIASYY"
     gene            472..1290
                     /locus_tag="KPH29_002179"
     CDS             472..1290
                     /locus_tag="KPH29_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG1467768.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIIGNSYEESILDFFCESNEDNYMTEISIQVSR"
     gene            complement(1423..3543)
                     /gene="feoB"
                     /locus_tag="KPH29_002180"
     CDS             complement(1423..3543)
                     /gene="feoB"
                     /locus_tag="KPH29_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBG1467769.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKDSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(3569..3796)
                     /locus_tag="KPH29_002181"
     CDS             complement(3569..3796)
                     /locus_tag="KPH29_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG1467770.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            complement(4123..4563)
                     /locus_tag="KPH29_002182"
     CDS             complement(4123..4563)
                     /locus_tag="KPH29_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG1467771.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKS
                     SYHIFLKENGLICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     INLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            4715..5704
                     /locus_tag="KPH29_002183"
     CDS             4715..5704
                     /locus_tag="KPH29_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HBG1467772.1"
                     /translation="MIYGGSVLNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDF
                     IVPVFFCIICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINK
                     PTMVMDDLVYGSVFGVIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLM
                     IALFPFGMVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRSLNQFLCCSKKAFYICV
                     SYLAIFEVLKLLNVKVDSVYLKTIFISITYISVYFIIRDSCMSLQNFIIAKFRKLLYA
                     RILFLIIILLLFFIFDVTVISLIIINAFLNKKINIRLSQSNIVNKQINLLIEK"
     misc_binding    5856..6108
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6180..7388
                     /locus_tag="KPH29_002184"
     CDS             6180..7388
                     /locus_tag="KPH29_002184"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBG1467773.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMNLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     gene            complement(7449..8330)
                     /locus_tag="KPH29_002185"
     CDS             complement(7449..8330)
                     /locus_tag="KPH29_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBG1467774.1"
                     /translation="MVKKRKKKTVTLTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPGRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            8716..9660
                     /locus_tag="KPH29_002186"
     CDS             8716..9660
                     /locus_tag="KPH29_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBG1467775.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLI
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
     gene            9883..10428
                     /locus_tag="KPH29_002187"
     CDS             9883..10428
                     /locus_tag="KPH29_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBG1467776.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRHFGK"
     gene            10519..11667
                     /locus_tag="KPH29_002188"
     CDS             10519..11667
                     /locus_tag="KPH29_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBG1467777.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQNSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSKKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNRVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            11720..12985
                     /locus_tag="KPH29_002189"
     CDS             11720..12985
                     /locus_tag="KPH29_002189"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBG1467778.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(13271..14503)
                     /locus_tag="KPH29_002190"
     CDS             complement(13271..14503)
                     /locus_tag="KPH29_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1467779.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFAATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(14506..15180)
                     /locus_tag="KPH29_002191"
     CDS             complement(14506..15180)
                     /locus_tag="KPH29_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1467780.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLVN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            complement(15188..16417)
                     /locus_tag="KPH29_002192"
     CDS             complement(15188..16417)
                     /locus_tag="KPH29_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBG1467781.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALNSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            complement(16529..17533)
                     /locus_tag="KPH29_002193"
     CDS             complement(16529..17533)
                     /locus_tag="KPH29_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1467782.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTVSVIIFSPVDVFLTDTILYI
                     LVVNVVFLFLYLFISYIKKNKFLDKIKNDTFKINMNDIELARCKNEEKIYLNIIKNHQ
                     YQCNNIINNNIEKFEENKEILNMWVHDVKIPIAIIKLILEQNENLINEKISDDIDSEI
                     FRIENSVEKILYLSRLEDFHKDFLIQEVNIEKIIRNIIKKYSKYFISKKIILDLNNLN
                     FKVLSDEKWLYFIFEQLLSNSLKYSSLGDNISIYCKRTKNYLQLRVEDTGCGIKKEDL
                     NRIFNKGFTGNNGRNNSKSTGLGLYLVKELCDKLGHKIDVDSEYNQYTKFTITFKCDL
                     "
     gene            complement(17557..18231)
                     /locus_tag="KPH29_002194"
     CDS             complement(17557..18231)
                     /locus_tag="KPH29_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1467783.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
     gene            18519..19286
                     /locus_tag="KPH29_002195"
     CDS             18519..19286
                     /locus_tag="KPH29_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1467784.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALKGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTMDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
     gene            19273..21210
                     /locus_tag="KPH29_002196"
     CDS             19273..21210
                     /locus_tag="KPH29_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1467785.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMIVVNMNLSIKALVKTLAVFLI
                     IFIVIGIRNTIVIRNKRIIELFNKYPEKYKFKKFIKLKCVLGVLLIAISYLMSISYFI
                     AENIMLSVFILVTIIPGTFLFFSSTMYMIIDAVKKRKSFYYKGQNLIAFSELGFKLKS
                     NSSVLAVIAILIATSVTMLGFTISLYYDIDRNISENYKYSYTINLGNSYVNNEIDKLL
                     YKYKKDNKIIFDKTIELIDKDINIEMYYKNGNSYIEDRTSVDIIRESDFKKLRQYQNN
                     NYEELTSKESVYYVSDSYKKMFFKDFEIEKINLYIKDTNEYDINLFKVQKSILEPEIN
                     SQATFDLIVVKDDVFNQLKLHGNSRLIRLIDIKNEKKELDLTLKFKNIVSENMKFTYP
                     FNFTSSIESYNNLIKLSGLMLFIGIFLSVVFLLCTGSIILFKQLSNIYDDKERYIMLI
                     KLGANNKDIEKIISKQLKIIFLMPLVVGTVHNLFAMSIAQKFIPRSLLVPIIITLVIY
                     FIGYFIYYFLTLKYALNMIKE"
     gene            21435..23048
                     /gene="ggt"
                     /locus_tag="KPH29_002197"
     CDS             21435..23048
                     /gene="ggt"
                     /locus_tag="KPH29_002197"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HBG1467786.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(23181..24032)
                     /locus_tag="KPH29_002198"
     CDS             complement(23181..24032)
                     /locus_tag="KPH29_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HBG1467787.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNDLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
BASE COUNT         8958 a         3066 c         3636 g         8481 t
ORIGIN      
        1 aataatatga tgctattgtt ataatagata ctaatgctaa tgatgttatt gcagtatcta
       61 atcttcttat taataaactc atttctatat attcaagttc tattaaatat tgataaaata
      121 tttctagttt aacagctttt tctctatcta tatttccata ctttaactct aatttatcta
      181 aagatttttt attacaataa ttaactttat gtttaattaa atatcttatt gtagcaccta
      241 ttacaatttt cactctaaca agcataaata taatattcca tccaaaagtt aagaggggat
      301 atctttttta tgagaggaat ggataaaata tttataactg acatctaact gatatttaag
      361 ctttaaaatc aaaaccatat tgattttcta aaataataac tacttgattt agtaaataat
      421 aaagaatata gtaactatat agacaacaaa aagaaaagga gtgtataacc cttggaaaaa
      481 cagtatttta caacaggtga atttgctaaa ttgtgtggta tatctaagca gacattaatt
      541 ttctatgaca aaataggtat tttttctcct gaatataaag ataaaaataa ttatagatat
      601 tactcagttt atcaatatga tacacttgat atattacaat cattaagaga aataggaatg
      661 agtttagagg aaataaaaga gtatatacaa aatagaactc cccagctttg tgtgaaaatg
      721 cttaaagaag aagaaaaaaa gataagagaa aaaataaaaa aactaagaaa gataagctct
      781 aagatgcaaa atagaataaa tataacagtt gaaggaattt ctaaaatgaa tagtgaagaa
      841 atttatatat ccaaaaagtt tgaagaatat ttagttctat caagcgattt gagtaatatg
      901 agtaatagtg attttatgat ggaattaata gatttcacca attattgtaa atcgaaaaat
      961 ttgtatcaag gatatgagtt gggagtaata gtaagtaatg aaaatataaa aaatggggac
     1021 tatttaagta tatcaagttt ttatttgaag atagataaaa aaataaagga taaaaagcta
     1081 tatgtaaagc cagaaggaat gtatgcttgc ataaaacata ttggaaaata tgaggattca
     1141 tataaatctt acgaaaaatt gaaaaaatat atatatgaaa atgaatataa aattattgga
     1201 aattcgtatg aggagagtat acttgacttt ttttgtgaaa gtaatgaaga taattatatg
     1261 actgaaattt caattcaagt atccagataa gaattaaaat agagatatat tcaaatgtta
     1321 agtaatttta cttatcattg aatatatctc tatgctttat aataaagtat ataaattttt
     1381 aaatagtttc tctaatatat gtctaatgtc agttaatact aattatataa ataaattgta
     1441 aactgtagtt gttaaaaaac aaacaacaaa agcaagtata gtaggcatta agaatcctaa
     1501 ggcagtccat tttaaactac cagtttcttt ttttattgtt aaaagtgttg ttgcacaagg
     1561 ccaatgcagt aaactgaaca gcataacatt taaagctgtc aaataagtcc atccatgttc
     1621 tctaagaact tgacctaaag cactaaagct atctaattct atcatagaac cagttgctag
     1681 gtaagccatt aaaagtatag gtacaactat ctcatttgct gggaatccaa gaataaaagc
     1741 aagtaatata aaaccatcaa gaccaattaa tttagctaaa gggtctaaaa agtttgcaac
     1801 atgtgataat atgcttaaat ctcctatata tatatttgca aatatccaag ttatgacacc
     1861 agcaggtatg gctaccatta cagcccttcc taaaacaaat attgttctat ctattataga
     1921 ggtatataaa gttcttccta tttgaggaac tctataagga ggaagttcta atgtaaatgt
     1981 agacggaact ccttttaata gcgtcttaga aagagtataa gaaaccaaca atgttattat
     2041 aacacctaga attatcagta aagttatgca aagtgcagtt gctacgcttg atacgaaaga
     2101 gttggttata actgagctaa aaaatattgt agatatagct atcaaagtag gaaaacgacc
     2161 attacaagga acaaagttgt ttgtaagtat agctataagt ctttcccttg gagagtcaat
     2221 tatcctacaa ccaataactc cagctgcatt acatccaaat cccatacaca tagtcaaaca
     2281 ctgttttcca tgtgcacaag cttttttaaa taggtggtct aagttaaatg caactcttgg
     2341 caaatatcca aaatcctcaa gtagggtaaa taatggaaaa aatattgcca taggaggtag
     2401 cattacagag attacccatg ctaatgttct gtataatcct aaaaccaaca tatcatttaa
     2461 ccatgatgga caattaatac tatttaatat acccgaaata cttggctcaa atccgagtaa
     2521 taaattagat aataaagtgg atggatagtt agccccttct atagtaatcc acaatatagt
     2581 acctaataat aagagcatta ttggaagccc aaatatttta gatgttaata ttttatctac
     2641 tttttcttct ctatcagttg attttttagc aacattagaa caacactcat cactcagttt
     2701 tttagcataa tcatagttga ttttagtaaa ctcatctctt attttctgtt tatttatatt
     2761 gtctggaatc tttttcttta cttcattaat agcatctata gaatctttgt ctatataatt
     2821 actcatagat tcaaatatac tttcatcgcc atcaataagt ctaagtccca accatctaga
     2881 atttatacct ggtataatat tatccaattc tggttgaatt gattttacca cattctctat
     2941 attttcattg tatctaacag gtttattatt taatttgtat ttgtcaaaag atacatcatt
     3001 taaagtatcc aatagttcat ccataccaga gccatttctt gcagcagtta aaataactgg
     3061 aataccaaga gaatcctcta aaagtttttt atcaatagta atgccttttt ttctagcttc
     3121 atctattaaa ttaatacaca aaataacttt atctgtaagt tccatcactt gaaatactaa
     3181 atttaaattt ctttcaagac atgtagcatc acatactact attactgcat ctggattacc
     3241 aaaacatata aaatcacgag ctacaatttc ttcttgtgat aaggcaaata aagaatatgt
     3301 tcctggtaaa tctattaatg catattcggt atttttatat ttaaaattac cacgagctgt
     3361 agctacagtt tttcctggcc agtttccagt atgctgtcta agccctgtaa gatgattaaa
     3421 tacagtactt ttacctgtat ttggattgcc agccaatgca attacaaact ggtcttcctt
     3481 attgtctata tcaaacatat cttttaaaga actcatttta gttgagttat gtgtcaaccc
     3541 catattaata tcctcctgct agaaattttt aaatgtcaga aactaatatt aagctacttt
     3601 cttcttgtcg tagagctatc ttactacctc tgattttata gacagttaaa ttattttttg
     3661 gaccttttct aactacatct ataacagctc ctctagtaag accaagcgct aacattcttt
     3721 ctcttaaatt accactagat aatatatctt caactttgac agtttttttc acttgaatat
     3781 cgttaaggtt tttcatagta gcacctccat gcttgaaatt tagcctgggc taatcttctt
     3841 attctaaact atgcattata ttattacctt gttactttta caaaaaaaag atagacaaga
     3901 tgcctatcat acaaagttgg gtataattct tacttttggg gaaagtagaa tatagaataa
     3961 aaattaacac tttgaatcca tataattatt gaattttgat aataaaaaag tcagaaagca
     4021 aaagcctcct gactctatgt aaggtatttt caaataccta aatatcaatt caatatttaa
     4081 tttatactta aattatatat taacaatatt agcttgtcaa cactataatc tcattttaat
     4141 atgttcaata cctgcttcgt cgtatacttc tgagatttct ttaaaaccac aagataagta
     4201 aaggttcttt atataagttt gtgctgataa ggttatatta ttttcgtgta agttaatttt
     4261 tataaaatct atagcacaat ccatcatttg ttttgctaat ccttttcttc tatgactaga
     4321 aagaactaaa actctaccaa tggaaacatc attataagag ctgtattctt taggaattat
     4381 tctagcataa gcacaaatca atccattttc ttttaaaaat atgtgataag agcttttatc
     4441 tatatcatca taatcgttta atgaaaatat cttctgttca caagcaaata cctcatatct
     4501 tgattttgct atttcataaa attcatctaa actcaaatca ttaaagtgtt taattttaaa
     4561 catagtaatt acctcctagc atcttaaaaa actgaatatt aagatacatt ataccatttt
     4621 taacataaat acctttatta aaatttatat ttattaaaaa tatttttaaa aagtgatata
     4681 ctatattaaa aacaaaactt tagttagaaa atttatgatt tatggaggca gtgttttgaa
     4741 tactactaaa aaattaactg aagcagcttt attgtcatct ttattcatag tagtaacaat
     4801 tatagctgtg agtactggat ttggatatgc tatttactta gattttatag tgccagtatt
     4861 tttttgtata atctgcttga aatgtgattt aaagtatacc attttatctg gaattacttc
     4921 acttttaata atttctctgg tacttggaaa tctaggaact gctatttggg caagtcagag
     4981 tgtattactt ggtataatgt gtggctatct aataaataag cctacaatgg ttatggatga
     5041 tttagtatat ggttcagtat ttggggttat tgtaatggtt ttcattgata tatatgcttc
     5101 aacattaata ggttatagtt ttatgaaaga atttcaagga tatagcaaat ggatttattt
     5161 taatgggtat acaaatttta tttattattt aatgatagca ttgtttcctt ttggtatggt
     5221 gttttgcata tatttattaa gtttaatttt agggaataaa ttgcatattt tagattctaa
     5281 ttctttaaaa aaatttttaa tatttaagaa ttttagaagt ttaaaccaat ttttatgttg
     5341 ctcaaaaaaa gctttttaca tatgtgtttc ttatctagcc atttttgaag ttctaaaact
     5401 gttgaatgtt aaagttgatt ctgtttattt aaaaacaatc tttatatcaa taacctatat
     5461 aagtgtctat tttatcatta gagactcatg tatgtcacta caaaacttta taatagctaa
     5521 gtttagaaaa ttattatatg ctagaatttt atttttgata ataattttat tgctgttttt
     5581 tatatttgat gtaacagtta ttagtttaat aattataaac gcatttttaa ataagaaaat
     5641 aaatataaga ttgagtcaga gtaatatagt caataaacaa ataaatcttt tgattgaaaa
     5701 ataaactttg ggtattggta aaaaatataa aaaagtagta taatgtaaaa aaacatgtat
     5761 ataataataa aaaaggttga caaaatataa aaataataat atattatgag taagttaaga
     5821 aaaatttctt gtaattgatg aaataatctg aatatataaa tgctatgaag agaagaagta
     5881 gggctagatt ttatttaaca gagagttcca gtttggtgaa atggaataaa ttgatttagt
     5941 ttgaatacat ctcagagcac tatctctgaa agataacaac cattaagaga tatcggatta
     6001 gtacacgtta tagtacacga gtatatgtaa ttgtactcta agagattaat atcgtgagat
     6061 attgatgaac taaggtggta acacgtaagc aatgctttcg tccttttaag aggatgaaag
     6121 ctttttttat tataaaaaaa aaattataga ttatataaat attttaggag gaatgaaaaa
     6181 tgaaatcgat agacgagcaa atgcgaataa ttatgaaagg tgtagatgat ttaatagatg
     6241 agaaagagtt aagggaaaag cttattaaat cagaaaaaga aggaaagcct atgatagtaa
     6301 aattgggtct agacccaagt gctccagata tacatttagg gcatacagta gttcttagaa
     6361 agatgaaaca attacaagat ttaggtcatc aaatagtaat aataattgga gattttacag
     6421 gaaaaattgg agacccaact ggaaaatcta aagctagaaa agctttaaca acagagcaag
     6481 ttcttgcaaa tgcaaaaact tatgaagaac aaattttcaa agttttagat aaagaaaaaa
     6541 ctattgtaag atttaatagc gagtggttag ccaagttaaa ttttgaagat gtaataaaat
     6601 tagcagcaac tattactgtt gcgagaatgt tagaaagaga agattttaaa aagagatatg
     6661 aaggacaaat gccaatatca gtacatgagt tcttctaccc attaatgcaa gcatatgact
     6721 ctatagcatt agaagctgat atagaacttg gtggaactga ccaaagattc aatttactaa
     6781 tgggaagatc attacaaaga gaatttggta tggagtctca aatagtaata atgatgcctt
     6841 taatagaagg attagatggt aaagaaaaaa tgagtaagag tttaggtaac tatattggaa
     6901 tagatgaaga agcgggaata atgtaccaaa aatctatgga aattcctgat gaacttataa
     6961 ttaaatacta caatttagtg acagatgttc atcctgatga agttaataaa atagaatctc
     7021 agttgaaaga gggttcagta aatccaagag atataaagat gaacttagca agagaaatag
     7081 taacattata ccatggagaa gaatctgcta aagaagctga agaaagattt aaatctgtat
     7141 tccaaaaagg tcaaatacct gaagatatac aaacaattca agttaaggaa gatggatttg
     7201 acttaatcga agtactagtt tctaatgaga ttgttaagag taagagtgaa gttagaagat
     7261 tagcttctca aggtggagta aaggtaaatg gtgaaaaagt agaggattta agcactattg
     7321 taaaagagag tgaattagtt gttcaaatag gaaagaaaaa atttgtaaaa atcgagctag
     7381 taaaatagag taaagtaaaa gcaagtctat tgagtataaa atgtacattc aataaacttg
     7441 cttttttatt atattaaaat tttaaattga taaccattgt ctttaaaata tgttataatt
     7501 tggtccaatg catttactgt ttcttgtttt ccataagtat catgcattaa aagaacaacc
     7561 atttctttgc cttcagaaga tttctttgca tgttcaaata attcttgtgc atttttcttt
     7621 ttaccttctg catcagcatt taaagcgttc caatcaattg atgccatgtt tttctctttt
     7681 aagtagtttc ctagaggttc catatttttc catgacatat acccgcctgg gcatctaact
     7741 acatttgagg aaaaattctt tcctaagact tttttcatag cttcatcagt tttgttaagt
     7801 tcatttacga atgtatcgag atttaggctt ctgccaggat atagtttttt atagtcatgg
     7861 gtataagagt gatgggctat tgcatttcct tcgtcaaatg ttctttttaa aatttcatta
     7921 gctccttttc tttcaagaga atcaccctta ataaagaaag ttcctctaac accatgtctt
     7981 ttcaagatat ctagaacttg tggagtatta gtagtagatg gtccatcatc aaatgtaaga
     8041 aagactatct tttctccatt gtttgaatag tcgtatttgt taagtttgtc ccaaactatt
     8101 tttgcgtcat atccatattc ttttacagct tctttttttg caccaataat aggttcattt
     8161 ttttcttttt caattctttc tttttctaat ctttcagctt taagtctttc ttgagtttct
     8221 ttcatttctt tttttctaga catgtccgcc catgccatag aaaaaatacc aataactatt
     8281 acaacaagta tagtaagtgt aacagttttc ttttttcttt tttttaccat aattacagca
     8341 ttcccccttt tgtcgaattg tatcaaaaat atattataat tatatcgaag aaagtagaaa
     8401 aagtaaaccg acaaagttgt aactaagata actaaagtgt aacttatatg acattatttg
     8461 cataatatta aagtatcata tatattaaaa atatctataa aaaaatttag tataggtaaa
     8521 aaaatataaa aaatataaaa atttattaaa aaggtgttta caaaaaatga ttaatatatt
     8581 actgttaaat agtggatata aatttgagct caaaatgtat gtaaatttag atttaaaata
     8641 ctgaaaaaat ttactatatt aatttatagt aatgcttata gaaaaactct cttgctgttt
     8701 agggggttta taattttgca aaaaaataaa aaggaggggg ctttattgaa aaaatacttt
     8761 ggagaaatag gactaatatt tatagcaatt atatggggaa gtggctttgt agcaacccaa
     8821 tttgcattag acggtgggct tacacctcta caaatcataa ctttaagatt tttcttggca
     8881 gctataatta tgaatttact gttttttaaa cagattcgtg ccaatatggg taaaaaatta
     8941 ttaaaagcag gagggatttt aggaatattt ttatttctag cattcacagt acaaactatt
     9001 ggacttatgt acactacacc atctaaaaat gcatttataa cagcagctaa tgttgtaata
     9061 gttccgttca taggatttat actttataga agaaaacttg ataaaatagg aataataagt
     9121 agtttggtag ctcttatagg aattgggata ctttctttag aagctgattt ctctattaat
     9181 tttggagatt ttctaacact tatatgttca ttcggatttg catttcatat attctttaca
     9241 agtgagtttg caaaagataa caacccaatg gcattgacag ctattcaatt tacagtagcg
     9301 tttttaatgt ctgttgtagt tcaaacattt gcaggtcaat taaagatgga agctgaatta
     9361 tctggttata tgggaactat gtatctagct gtatttagta ctacaattgg atttttattt
     9421 cagactatat gtcaaaaaag agttgatgga actagaacag ctataatatt atccacagag
     9481 gctgtatttg gtacaatatt ttctataata attctaaagg aacttattac agcaaaattg
     9541 attattggta gtatcttgat atttgttgca atcataactg cagaaactaa attatctttt
     9601 ttaaagagca aaaaagtgaa acttaaagat tcagaggagt cttcattaga aagtatttaa
     9661 tatgacaact atttcctaat taatcaaata agttatgtat tatgtagaaa tttgacatca
     9721 aaacttggtt gatactggtg tttcaaagtg ataaaattaa ttaggaaata aattttattt
     9781 ttagtgttta agctataaag tctggggtat atatattctt gtagcaagtt attaaactta
     9841 aaaataaaaa tttaattata taaaattggg aggaaattaa ttatgaaaaa atttgtttgt
     9901 acagtatgtg gatatataca tgaaggagat gctgcaccag cacaatgtcc agtatgtaaa
     9961 gttggagctg ataaatttga agaaatgaaa ggcgaaatgg tttgggctga tgaacataga
    10021 ataggagtag ctcaaggtgt agatgcagaa ataatcgaag gattaagagc taactttact
    10081 ggtgagtgta cagaagtagg aatgtattta gcaatgagta gacaagctga tagagaaggt
    10141 tatccagaag tagctgaagc atataagaga atagcttttg aagaggctga acatgctgct
    10201 aaatttgcag aacttcttgg agaagttgta gttgcagata caaaagaaaa cttaagagtt
    10261 agagttgatg ctgagtatgg tgcaactgat ggaaaattaa aattagctaa gagagctaaa
    10321 gaattaggat tagatgctat acatgataca gtacatgaaa tgtgtaaaga cgaagctaga
    10381 catggtaaag cattcttagg attattaaac agacattttg gaaaataata attaaaacat
    10441 agattaaaag gagaaacttt ggtttctcct tttttaaatt atacaaataa actgttgtga
    10501 tagaaggatg gagataatat gaagtcggta tctgttagga gttgtaaaac ttacaattat
    10561 gaagaagtaa agttatctat tgaaaaaaat ttagaggata ttggtggtat agataagttt
    10621 gttaaaaaag gaagcaaagt acttataaaa ccaaatttat taatgaaaaa aaagcctgaa
    10681 gaagctacaa ctacacatcc tatggttgta aaagttgtat gtgaaaaact tttagagtta
    10741 aattgtgata ttacaatagc agatagccct ggtggtccat ataatcaaaa ttctcttaag
    10801 agcatttata aggcatctgg aattgaagaa gtagcaaatg aactgggcat taagttaaat
    10861 tatgatgttt cagatgttaa gatacatagt aaaaaagcac atgttctaaa atatatggat
    10921 attattaaac caatagtaga ttcagattat ataataaatc tttgtaaatt aaagactcat
    10981 ataatggcaa cctttactgg tggtacaaaa aacttatatg gatgcatagc aggattgaaa
    11041 aaagcagaga tacattatag atttcctaca gaagagttgt tttgcgaaaa tgtactttta
    11101 gatatttgtg attatataaa accaacattg acaattatgg atggtatagt aggtatggaa
    11161 ggtgatggac cttcagctgg tactactcga gaaataggag ttttattagc atcacaaaat
    11221 ccatatgcta tagatgttgt tgcatgtaag attataagtt tacttccaaa tagagttgct
    11281 acaattagag gtgctattaa gagaggctat atacaagaag atttttcaga tattgaaatt
    11341 ttaggtgaag atataaaaga tttaattata aaaaattaca agattccaaa tgtaagtaag
    11401 gatttaagat tgttttccgt aaagctacca aaatttttaa atgaacctat ttcaaagctt
    11461 ataacaccaa agcctgtggt aagatttcaa gattgcataa aatgtggaaa atgcaaggaa
    11521 gcttgtcctg catccactat agagataggg actaaaggtg ctattataga cttaagtaaa
    11581 tgtataagat gttactgttg ccatgaactt tgtcctaaaa aggctattga tgtcaaacgt
    11641 aattttgtat ttaagtatgt aaaataatgt ataatattat atgagtctat ataaattaaa
    11701 aatataaagg agaaataata tgattttaat taaaaatggt agagtaatag accctaaaag
    11761 tcaaagagat gagaatattg acttaattat aaaagaaaat aaaatttata aaataggcaa
    11821 gtttgatgaa agtgatgaat atgaaaaaat aatagatgct agtggtaatg tagttgctcc
    11881 aggtttagtg gatgtacatg tacactttag agaccctggg tttacatata aagaagatat
    11941 agaaagtgga gctaaatctg ctgctagagg tggatttaca actgttatat gtatggcaaa
    12001 tactaatcct attgtagata atgaagatac ttttaattat gttaaagaaa agtcaaaaaa
    12061 tgcatgtata aatgttttac aagcagcagc tataactaaa ggttttgaag gtaaagagtt
    12121 agttgatatg gaagctctta aaaaagctgg tgtaccaggg tttactgatg atggattacc
    12181 tttaatggat agcaatctaa ttatggaggc aatgattaag gcaaaagagt tggatgtacc
    12241 acttagtttt catgaagaag acccttcttt agttggaaat ccaggtgtaa atgcaggaaa
    12301 ggtagcttca gaactaggat taaaaggtgc atcaagtgta gcagaagatg ttatggtagc
    12361 cagagattgt atgttggcac taaagactgg tgctaaagtt gatatacaac atataagttc
    12421 aggtgtatct gttgatatgg ttaggtttgc aaaatactta ggtgcaaatg tagttgcaga
    12481 agcttcacca catcacttta cattgactga ggaagatgta ttggagtttg gaacaaatgc
    12541 aaaaatgaat ccacctctaa gaagtaaatg ggacagagat aagattatag agggattaaa
    12601 tgatggtact attgaaataa tagcaacgga ccatgcacct catagtaaag aagaaaaaga
    12661 tagagagttt ataaaagctc caagtggaat tattgggctt gaaacttctt tagctcttgg
    12721 aataacaaat ttggtgcata aaaatcatct tagtatgatg cagttaatag aaaaaatgtc
    12781 tataaatcct gcaaagttgt acaatttaaa tattggattt atagaagaag gggctgtagc
    12841 agacattgtt atattcaatc cagaagaaga atggacagtt gaaagctttg tttctaaagc
    12901 tgataattca ccgtttaaag gtaaatcttt atatggaaag gttaattata caatatgtaa
    12961 tggggagatt gtatataacg cctaatttaa gattattttt ttaaggcgga gtgtacaatg
    13021 attgaaagta aaagattgta tatcagcaga atgagattga ttaatcaaaa ttggaagtat
    13081 tagtttgaac aattagaaca taacatcttg gtaatttaga tatagtaagg aagagaatcc
    13141 tttttatgaa agaacgcact attatatttg gaaagattaa gttcctaatc tatcatatat
    13201 aatagtgcgt tatatattta agtatgtttt atataaacta atctaaaaat cgaaaatctt
    13261 tatacaattt ctacttataa attgccttta tagggtctag tttagaagct ttaaaggctg
    13321 gaatcagacc aaatacaact ccagtcaata tagtagctac aattgcataa aataagctat
    13381 ttaagctagg tatagcttca aaacctatat atttagaaac atagttagta gcagcaaatc
    13441 caactatagt tcctaaaatt ccaccacata ctgttataaa aacagcttca actaaaaatt
    13501 gaaataatat agacctaggt tttgctccaa tagcacgtct tataccaatt tctctttgtc
    13561 tttccataac agatacatac attatattca ttacaccaat accaccaaca aacattgcaa
    13621 ctacagttat tatagataca tatttgttta cattggaatt aatgctttca agataagctg
    13681 tctgttcagt tgggtctggt gtagtataag aaccattgat accagggtgc atctcatata
    13741 atttagctat aacattgttt gcaacttcat ttacattata gcctttagaa gaaactaagt
    13801 ctaactggta aatttcattt gaataagaat tttgactcat taaagtgtca aaagcctttt
    13861 taggtactag ggaagtagta aattgcatat cttggtaacc tccaaagaat cctccagctt
    13921 ggttttgctg agaatcatct aataccccta ttatttcaaa tatagttcca tttatgttga
    13981 taccatgacc taaggcatct tctggatttt caaaaatttc acttgtactt tgtaatgtaa
    14041 gtaaaattac ttttcttttt tcatcatcta aagaaaaatc tctaccacaa attaaattta
    14101 ttttagaatc tttttttact ggtccaacat caacatacgt agttttttta tcaaaagatg
    14161 cttgtgaaga gtaaactgaa tcaaggttaa acccatctct tgaaggagct attctttcta
    14221 ctccttcaac aaatgataat tcttctaaat cttttgcatt aaagggtttt aaaaaaatac
    14281 tcatatcatc agtaagacct gtgttatcgg ctgattcaaa cgaaatagta gttttattag
    14341 ggtttacatt gtttacagat ttcttaattt ctttttgaaa tccattacct atagaactaa
    14401 caagtattac tgaagttatt cctattataa tccagagtaa agctacaaat acacgaagct
    14461 tgtgaccttt taaattggcc aaagaatttt ttaataaact catgattaca cctccgaagt
    14521 aaacacacca tctttaagtc gtataacctt agttgcatac ttagttaaat cttgatcatg
    14581 tgtaaccatt attatagtct taccttgttt gttgagcctt gttaaaagct ccataatttc
    14641 tctgctagtt tcactatcta atgccccagt cggttcatca gcaaagatta tctgagggtc
    14701 atttactagg caacgtgcaa tggctacacg ctgttgttga ccaccagata attgaagtgg
    14761 tttctgctta agcttgtcca aaagcccaac agaacttaat ttatctttaa caatttcttc
    14821 tctattagat ttttttagtg ctttattgta aattagaggc aactctacat tttgatatac
    14881 atttagagtt tctattaaat taaattgttg gaaaacaaat ccaatattaa tatttctaaa
    14941 aacagaacgt tcattttcac tcaaattagt tacatctgtt ccatcaaata tatatcttcc
    15001 ttcatcaaat acatctagaa aacctaagat attaagtaaa gtagttttac cactaccaga
    15061 cttacccatt atcatgacaa attctccaga ttcaatttct aaatttaagg attttaaaac
    15121 atgtaattca tctttcccta ctttgtaata tttttgaata ttttctaatt taattaacat
    15181 atgtaaatta attcacatcc atagtaactt cttctacttt tttgtttgga gtagtatctc
    15241 cattattaga accagcagtt acgccatcgc cttgaactgg gtctccttct ttcatttctt
    15301 tagtagggtg ttttattatt atatcgcctt gttcaagacc gcctctaact acagcaaaat
    15361 catcattttg acttgctaca tctacaattt gctttttcaa tataccatct aaatccttaa
    15421 atacatatga gtgtttgttg tctttaagta cacaagatgc tggtatttta aagcttgaat
    15481 tagctacttc aagtgatgct tgaagatgga atccatttac taatccttct tgattatcaa
    15541 atgctatgtt tatatcataa taagatagtg tattctgctg agctcccatt tcagtatttg
    15601 gagtagatgg tttatcagat ataaaactta ttctaccagt aagcttttgg tcagttgaaa
    15661 aaactagtat attgaccatt tggtcaatct tcattttagg taaatcttgt tcacttgctt
    15721 gtcctttcat ataaaactct agaccttgaa tagtcataaa tgaaactggt tggtctggat
    15781 ttccagtttg gtcattcaag tatactgtgc catcaaaagg agcagtaact ctgtcataag
    15841 cttttttatc cagagaagct atctgagagt ttaaagcagt tatctgtgca tttatagaag
    15901 caatagcatc tttattttca gcaatatcag aaagtgatat tttttgtttt ttgtattgac
    15961 taagttgtga ttttaaacta tctatttctg ctattatagt tggattttta gcagtaaaaa
    16021 gaaggtctcc ttgatttact ttttgaccat ttgtaacatt aacatcactt aactcataat
    16081 ctccagatat attgaagtcc ttagtttgct taggaacaac catcccattt ataaatattt
    16141 tttcattttc aggaatagta tatgtttcga taaaattttc attagcttgt aattcagtgt
    16201 ttttcgaatc aacataagca agaactccta gaattaaaag tatgattact gctattcctg
    16261 catatatttt gaatctctta tttttaaagg gattgttttt aaaaccttta tttttagact
    16321 tgaagccact acttccaaaa ctgctatttc taaagttgtt acttttaaaa ctgttatttc
    16381 taaatctttt atttttaaat ttttttagaa aagacatgtc ttcctccttt ttataaaaac
    16441 tacttaatta tatactggga tgtacatata gaattactat aagaagttga atataagtta
    16501 atgaaaataa actatcaata ataagatact ataaatcaca tttgaatgta atagtaaatt
    16561 ttgtatattg gttgtactct gaatctacat caattttatg accaagctta tcacaaagtt
    16621 cttttactaa ataaagccca agacctgtag atttagaatt gtttcttcca ttattacctg
    16681 taaatccctt attgaatatt cgatttaaat cttccttttt tataccacaa ccagtatctt
    16741 ccactcttaa ttgtaaataa tttttggttc ttttgcaata gatagatata ttatctccta
    16801 atgaggagta ttttagggaa ttactcaata attgctcgaa tatgaagtat agccattttt
    16861 catcagataa aaccttaaaa ttcaaattat ttaagtctaa aataattttt tttgaaataa
    16921 aatattttga gtattttttt ataatatttc taatgatttt ttcaatattt acctcttgaa
    16981 ttaaaaaatc cttatgaaaa tcttcgagtc ttgataaata taaaattttt tcaacggagt
    17041 tttcaattct aaatatttca ctatctatgt catctgaaat tttttcatta attaaatttt
    17101 cattttgttc aagaatgagt tttattatag ctataggtat ctttacatcg tgtacccaca
    17161 tatttaaaat ctctttgttt tcttcaaatt tctcgatatt attatttatt atgttattac
    17221 attgatattg atgattttta ataatgttca agtatatttt ttcttcattt ttacatctag
    17281 ctaattctat atcattcata ttaattttga atgtatcatt ttttatctta tctaaaaatt
    17341 tgttcttctt gatgtagcta ataaatagat ataaaaataa aaacacaaca tttacaacta
    17401 atatatataa aatagtatca gtcagaaaaa catctacagg agaaaatata ataactgata
    17461 ctgtaaaaat taagaaagta aaattgtata ctaaaaaacc tagtttatcc ttgagaaaat
    17521 ctttaaagct cataaattca cctacttaga attgttttaa ctgattatat aacccaatcc
    17581 tctttttgtt tctataaaat cttctaaacc aatttcattt aattttgagc gtattctatt
    17641 tacattgaca gttaatgtat tatcatctac aaaccaatca ttatcccata attttttcat
    17701 gagtttattc ctacttacta tctgcccctt atatttcata agctcatgaa gaattttgat
    17761 ttcatttttt gttagctcaa tagtattatc ttcatagttt attgttgcag ttgataaatc
    17821 taaagtaaca ccattgtgaa taatctgatt tgagttatta tcaacaaaat tataagttct
    17881 tcttaaaagg gcattaactt tagctactaa gacatctaca gaaaaaggtt tttgtatata
    17941 atcatctgca cctaaattta cacccattat tacatccata ttagaatttc ttgaagaaat
    18001 aaaaattata ggaacttttg aaatttttct tatttcatta caccaatgaa aaccatcata
    18061 aaatggaagg tttatgtcca ataaaactaa ttctgggtta tagtttttga actcttctat
    18121 tatattttca agattttcta tttgatgaac atcatgtccc catttagtaa gacactttgc
    18181 tatgatattt ttcaatgaga cgtcatcttc aactaccatt attttaaaca ttcttacctc
    18241 ctaattcaaa atagttatat taacataact atattctact tgataaatta aaatatttct
    18301 accttattac ctaaaataat tatattaata taactatatt ttacttgata aattatattt
    18361 taaacataat tgcaataaat ttgaagaaat ataattaata cttacgaaaa tgtaacctta
    18421 aattaatata attaaatgtt taaaagccat tttaatatat ataatatatt tgtgagaatt
    18481 gataaataac taattgtgat gaaaggtgat tattaactat ggaagaaata ttatctgttg
    18541 aaaatataaa aaaggagtat ggaagaaaag gttcaaaaca tgaagctcta aaaggaataa
    18601 ctttcaaggt ttataaagga gaatttgttg gtattatggg gtcttccggt gctggtaaat
    18661 caacactttt aaatattatt tctacaatgg atttaccttc atcaggagat atttatataa
    18721 atggaaagaa tactattaaa atgaagcaaa atgaattggc agattttaga cgtgataatc
    18781 taggctttgt atttcaagat tctaatcttt tggatacact tacaataaaa gaaaatataa
    18841 tgttgcctct ttctttaaaa aatgaaagag tatcagtaat agaaaataga ataaaagaga
    18901 tttcaaaaga attaaatata gaatctatat tagataaata tcctggcgaa gtttctggtg
    18961 gacaaaagca aagaggagct gtatgtagag ctatagcaac aaaaccatca ttagtacttg
    19021 cagatgagcc tacaggagca ttagattcta aatctgctag ggatttgttg aattgtctat
    19081 taaaattaaa caaagacagc aataaaacaa tattgatggt aactcatgat gctataagtg
    19141 cgagtttttg caatagaata ctctttataa aggatggaat aatattcaca gaaattgtaa
    19201 agggagaaag taacagagag ttctataata aaatagtaaa tacagtttcc ttaattggag
    19261 gagttaataa aaatgacttt atttagaatt gcaatgcaaa atgtaaagaa tagttttttt
    19321 aattatttaa tgtactttat ttcaattgta tttagtgtat ttgtattttt tagttttaaa
    19381 tctatagagt ataatgaagc gttgagttca cttggagaaa aaactaggat gagtattaat
    19441 acaagttcaa ttgtaatagt ggtatttgta tttttattta tatattattc aaactcattt
    19501 tttttcaata gacgtaggcg agaagtagga acttatagtt tacttggaat gaggaaaaat
    19561 cagataggaa aaatattttt atatgaaact tttttgatgg gtatagttgc aatactaata
    19621 ggtatatttt taggactgtt attttcaaag cttatgacta tgatgcttgt aaaattaatg
    19681 aatgaaatga tagtagtaaa tatgaactta agtataaagg ccttagttaa gacactagcg
    19741 gtatttttaa taatatttat agttattgga ataagaaata ctatagttat cagaaataag
    19801 agaattatag agttatttaa taaatatcca gaaaagtata agtttaaaaa atttataaag
    19861 ttaaaatgtg tacttggtgt tttattaata gctatttctt atttaatgtc aattagttat
    19921 tttattgctg aaaatattat gctttcagtt tttatattag ttacaataat acctggaact
    19981 tttttatttt tttcatcaac tatgtatatg attattgatg ctgttaagaa gagaaaaagt
    20041 ttttattata agggacagaa tttaattgca ttttctgaac ttggatttaa attaaagagc
    20101 aatagtagtg tattggctgt aatagcaata ttaatagcta cgagtgtaac tatgctaggg
    20161 tttaccatat cactttatta cgatatagat aggaacataa gtgaaaatta taaatacagt
    20221 tataccataa atttaggaaa ttcttatgta aataacgaaa ttgacaaatt attatataaa
    20281 tataaaaaag ataataagat aatctttgat aaaacaatag aacttataga caaagatata
    20341 aatattgaaa tgtattataa aaatggaaat agttatatag aagatagaac ttctgtagat
    20401 attattagag aaagtgattt taaaaaatta agacaatatc agaataacaa ttatgaagaa
    20461 ttaacttcaa aggaatctgt gtactatgtt tctgattcat acaaaaaaat gttttttaaa
    20521 gactttgaga ttgaaaaaat aaacttgtat attaaagata caaatgagta tgatataaat
    20581 ctgtttaagg tacaaaaaag catcttggag ccagaaataa attcacaagc tacgtttgat
    20641 ttaattgtag taaaagatga cgtatttaat cagttaaaat tacatggaaa ttctagatta
    20701 ataagattaa ttgatataaa aaatgaaaaa aaagaactgg atttgacttt aaaatttaaa
    20761 aatatagtaa gtgaaaatat gaaatttact tatcctttta attttacatc aagtattgaa
    20821 agttataata acttgataaa attaagtggt ctaatgctgt ttataggcat atttttgtct
    20881 gtagtatttc ttttatgtac aggtagtata atcttattta aacaattatc aaatatatat
    20941 gatgataagg aaagatatat tatgttaata aaacttggag caaataataa agatattgag
    21001 aaaataatat caaaacagtt aaaaattata tttttaatgc cattagttgt aggtacagtt
    21061 cataatttat ttgctatgtc tatagcacaa aagtttatac caaggtcatt acttgtacca
    21121 attatcataa ctctagtcat ttattttata ggatatttta tatattattt tttaacttta
    21181 aaatatgctc taaacatgat aaaagaataa attattttta agtcaaaaga attagatata
    21241 tgttttgaat aaaataactt atttaagtgc agatagaaaa tactagaatt gagtttaaga
    21301 tatttattta aagataaaat taaagttagg aaaaagtcta tattttatat cattttgtat
    21361 cagttacatt acttgatgtt atataataaa tctaagcaga atatcaaata aaaaatgacg
    21421 aggagctgaa atttatgaag tttaatgcct accaacataa atactcatca ataagaaatg
    21481 tcgtgtatgc taaaaatgga gcagttgcaa catcaacacc attagcatct caagctggac
    21541 ttgaaatact aaaaaagggt ggaaatgctg ttgatgcagc agttgcaaca gccgccacat
    21601 tagcagtagt agaacctaca agcaatggga ttggaggaga tgcatatgcc cttgtttgga
    21661 tagaggaaga aaaacgattg tatggtttaa attctagtgg ctttgctcca gaaaatatgg
    21721 aattaaaaaa ttataataat atgaaagaaa tgccaaaata tgggtttgga gcagtaactg
    21781 tacctggaat acccgcagct tggtcagaat taaataaaaa atatggtaaa cttagcttaa
    21841 tggaatgctt atctccagcg ataaactatg caagagaggg atacgtagta tctcctaatg
    21901 tagctaaggt atggaaaaaa tcatatgaac tctatgaaaa agaacttatt ggagaagagt
    21961 tcaaaccatg gtttgataca ttttctaaag atggtaaagc tccagaggct ggagatatat
    22021 ttatatgtga agaacaggca agtacattag aagagatagc aaatactcag gctgaaagct
    22081 tttatagagg aagacttgct gataaaatag atgaatattc taaaaaattt aatggggcta
    22141 taagaaagag tgacttagag tgtttttatc caacatgggt agaacctata agtacagaat
    22201 ataagggata taaaatattt gaaatcccac caaatggtca tggaataaca gttttaatgg
    22261 cacttaacat attaaaagaa ttggaattag aaggaaatat agaaaatgta gaagacatac
    22321 ataaaattat agagagttta aaattagctt ttgcagattc taaaacatat gtaactgata
    22381 tagagcatat gaaggtaaag attcaggaat tactaagcca agagtatgcc aaaaaaagaa
    22441 gtttattaat tgacaataaa gaggctttgt atccaactgc aggagagcca tattgtggtg
    22501 gtacagtata cctttgtact gcagataagg atggaaatat ggtttcatat atacagagca
    22561 attacataaa ttttggttca ggaattgtga taccaagaac tgggattgct ttacatagta
    22621 gaggaaataa ttttaattta gaccctaaac accacaatgt agtaaaacca tttaaaaaac
    22681 cttatcatac tataatacct ggatttttag gtaaagaaga taaagcaata ggtccatttg
    22741 gtgtaatggg agcttttatg caaccacaag gacatattca agtgttaaca aatatgattg
    22801 attttggatt aaatccacaa gaggcgctag atgctccaag atggcagtgg ataaaaggaa
    22861 aggaaataga agttgaacca gagatgccaa agcatataat agattcatta attgaaaagg
    22921 gtcatgaaat aaaagtaatt catgatactg tcgacatggg acgaggtcaa ataatattta
    22981 aaacagaaca ggaaagttat atttgtggga cggaatcaag atgtgatggt catattgcag
    23041 tttattaata ttatcaacta aaatatatat aataaattta aagagtatat gaaataatta
    23101 gtaaaggcga aagtctacaa tgactgacgc ctttacaaaa tttaagctat catatgctga
    23161 ttttatatag attatgagcc ttattcttga ccagttttga tttcattgtc atcataacta
    23221 atccagtcac taaagctacc aatataaatc ttgtgaggta tatccagttc tctaagagca
    23281 agactgttaa cacaagcaga tatccctgaa ccacaagata aaattacttc atcataatta
    23341 tttaaatctt taaagaattt ttttaaaaat tctatatctt taagtgagcc attttcaaag
    23401 tcacttttta ttaaatcctt acaaaaataa ttttttgcac ttggtatatg accagctttt
    23461 gagtaagcag gttcaactaa accctggtat ctctcattag cacggcaatc tattattaca
    23521 gtatcttttt tgtataattt agacttaaca tattccattg gaacaactaa atcattgttt
    23581 atatttggtc taatttcctt acctgtacaa cttggtatat tcactttctc ttccaattct
    23641 ccaccttcct ttacgaaaga tgttattccc ccatctagta catagacatt tttaagcccc
    23701 aaatgtttta attggaaaaa taatctgcaa gctccattta aatctccatc atcataagtt
    23761 acaatcgtag tgtcattatc aacacccatt ttttctaatt tttcttttag tattaaaggg
    23821 tcttgtaaag gatttttacc accatgttct tttgtaggag atgataaatc cttgtctata
    23881 tctaagataa aactgccttt gatatgtcct tttttataag aatcaatacc ataagttcta
    23941 ttgataagat caaatctgca atctattaca actagattat catttttttc tagttttgat
    24001 ataagttctt ttgctgaaat aatgtttttc aaagaatgta cctcctatgt ttatattatc
    24061 tttattttaa tttatattaa ttattaactt tataaactaa tattattata atttattaca
    24121 taagtaaata aatctttaga a
//
LOCUS       DAELXX010000060        23587 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.060, whole genome shotgun sequence.
ACCESSION   DAELXX010000060 DAELXX010000000
VERSION     DAELXX010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23587
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.060"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            147..1217
                     /locus_tag="KPH29_002199"
     CDS             147..1217
                     /locus_tag="KPH29_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG1467788.1"
                     /translation="MFDKTYINHKGHGKVNSGWGNSDIESIIGNSEAMLKVKERTKKL
                     AKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPENLLESELFGYIKG
                     AFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQERKFARIGSNKLIDL
                     DIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDDIEAIMMKFASKYS
                     LELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDGIIYKDMLPLDILN
                     YYNINGNVCKNKDINIIFEDDIVGGIVENQERILSIKELELTYINKLLNKYGRDTKTK
                     KKIAKDLGIGLATLYRKLEEEQ"
     gene            1294..1833
                     /locus_tag="KPH29_002200"
     CDS             1294..1833
                     /locus_tag="KPH29_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HBG1467789.1"
                     /translation="MQYAIELYYDKKTEKQLFDLSKKIADEKISTKYLEWKTRPHLTL
                     ACFNDVDEACCIDKLKGFAQNHKVIPAYIGSVGMFNDTKTVFVSPIMNSSMYQFQREL
                     YEVLQDFDSNGWEWYHPNRWVPHCTIALTGDDESEAFFRASDVLLREFKKISGEFVSI
                     GLVKITFPVEEIFTVNMNR"
     gene            2456..3670
                     /gene="dpaL"
                     /locus_tag="KPH29_002201"
     CDS             2456..3670
                     /gene="dpaL"
                     /locus_tag="KPH29_002201"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBG1467790.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLRDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKSLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGECQSK"
     gene            4003..5190
                     /locus_tag="KPH29_002202"
     CDS             4003..5190
                     /locus_tag="KPH29_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HBG1467791.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGINAVYKMANVIQKIQQLVPPT
                     HPVLGDGILVLTDIKSSPYPGASVVPDYCKATFDRRLLVGETREGVLAPIQALLDEMM
                     KEDPELNAKVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYNGLKEAGIDSE
                     ITQYSFCTNGSHYAGEAGIRTIGFGPSKENLAHTIDEYIELDQLFKGAEGYYGILKSV
                     YKK"
     gene            5206..6789
                     /locus_tag="KPH29_002203"
     CDS             5206..6789
                     /locus_tag="KPH29_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBG1467792.1"
                     /translation="MRTLIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKSLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            6817..8145
                     /gene="ssnA"
                     /locus_tag="KPH29_002204"
     CDS             6817..8145
                     /gene="ssnA"
                     /locus_tag="KPH29_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HBG1467793.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            8344..9696
                     /locus_tag="KPH29_002205"
     CDS             8344..9696
                     /locus_tag="KPH29_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBG1467794.1"
                     /translation="MSQDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            9888..10997
                     /locus_tag="KPH29_002206"
     CDS             9888..10997
                     /locus_tag="KPH29_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG1467795.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            11280..12671
                     /gene="hydA"
                     /locus_tag="KPH29_002207"
     CDS             11280..12671
                     /gene="hydA"
                     /locus_tag="KPH29_002207"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBG1467796.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNAAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            12665..15226
                     /gene="xdh"
                     /locus_tag="KPH29_002208"
     CDS             12665..15226
                     /gene="xdh"
                     /locus_tag="KPH29_002208"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HBG1467797.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEDDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETASLLPENIVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSVPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            15378..16724
                     /gene="rpoN"
                     /locus_tag="KPH29_002209"
     CDS             15378..16724
                     /gene="rpoN"
                     /locus_tag="KPH29_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBG1467798.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
     gene            17521..18543
                     /locus_tag="KPH29_002210"
     CDS             17521..18543
                     /locus_tag="KPH29_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG1467799.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            18603..19610
                     /gene="gap"
                     /locus_tag="KPH29_002211"
     CDS             18603..19610
                     /gene="gap"
                     /locus_tag="KPH29_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBG1467800.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            19832..21034
                     /locus_tag="KPH29_002212"
     CDS             19832..21034
                     /locus_tag="KPH29_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBG1467801.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKANNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            21060..21803
                     /locus_tag="KPH29_002213"
     CDS             21060..21803
                     /locus_tag="KPH29_002213"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBG1467802.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLSK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            21812..23344
                     /locus_tag="KPH29_002214"
     CDS             21812..23344
                     /locus_tag="KPH29_002214"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBG1467803.1"
                     /translation="MMKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTL
                     INASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFANKVLCEAMD
                     NAKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALE
                     YAKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAI
                     QNSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDG
                     FKREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAH
                     VTFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVL
                     NFANPDMVGHTGSIEAAIKAVETVDTCVGKLIDKIVELGGSAIITADHGNAEYMLDPE
                     TGKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTVLDMMKLEKPEEMTGHSLI
                     SK"
BASE COUNT         9470 a         2687 c         4733 g         6697 t
ORIGIN      
        1 tagaaaatat agtaaatata gtatctgttg aatcttcctc aaatggacat gaattactag
       61 aaattgatat tgataacaaa atttataata taaatgccaa aataattccg gtatatccat
      121 atataaatca atacgacaaa ataataatat ttgacaagac atatatcaat cataaaggac
      181 atggaaaagt caatagtggc tggggaaata gtgatataga gtctataatt ggaaattcag
      241 aggcaatgtt aaaagtaaaa gagagaacta agaaattagc aaagtcaaat tcaactgtac
      301 ttataactgg ggaaagtggt acgggtaaag agctaattgc tagggctata catgctgaag
      361 gtagcagatg gaacaagcca tttattgcga taaactgtgc tgctattcca gaaaatctcc
      421 ttgaaagtga gctatttggc tatataaaag gtgcatttag tggagctagt agtggaggta
      481 aagtaggtaa atttgaactt gcaaatgaag gtgttatatt tcttgatgaa ataggagatt
      541 tgtcaatgcc tttacaagca aagttactta gagttttaca agagagaaaa tttgcgagaa
      601 ttgggtcaaa taaacttata gatttagata taagagtgat tgctgctaca aataaaaact
      661 tattgaaatt agtaaatgaa ggtaagttta gggatgattt atactataga ttaaatgtaa
      721 ttcctataaa tttgccacct cttagggaaa gaaaggatga tattgaggca ataatgatga
      781 agtttgcttc taaatattct ttagaattag gtattcagtt aaataaaata gaagaaaatg
      841 taatgaatat gttgattaac tataattggc cgggaaatat aagggaactg gaaaatgcag
      901 ttgaatatat gatgaatttg gttggagatg atggaataat atataaagat atgcttccat
      961 tggatatttt aaattattat aatattaatg gaaatgtttg taaaaataag gatataaata
     1021 taatatttga agatgacata gttggaggga tagtagagaa tcaggaaagg atactttcaa
     1081 ttaaagaatt agagttgact tacataaaca agttgttgaa taagtatgga agagatacaa
     1141 agacgaagaa gaagatagca aaggatttag gtataggttt ggctacacta tatagaaaat
     1201 tggaagagga acagtagtaa tataatatta aaatgatagg tatttgtgtg tttttataga
     1261 taggtatttt tactttatgg tggaggtgtt attatgcaat atgcgataga gctatattat
     1321 gataaaaaaa cggaaaagca actttttgat ttatctaaaa aaatcgctga tgaaaaaatt
     1381 agtacgaagt acttagaatg gaaaacaaga cctcatctta ctttggcttg tttcaatgat
     1441 gtggatgagg cttgttgcat agacaagcta aaggggtttg cacaaaacca taaggtaata
     1501 cctgcttaca ttggttcagt aggcatgttt aatgatacta aaacggtgtt tgtatcacct
     1561 ataatgaata gcagtatgta tcagtttcag agagaattat atgaggtact acaagatttt
     1621 gattcaaatg gttgggaatg gtaccacccc aatcgttggg taccccattg cacaatagca
     1681 cttactggag atgatgaaag tgaggctttt tttcgggcaa gtgatgtatt actgcgtgag
     1741 ttcaaaaaaa taagtggtga gtttgtctca ataggattgg ttaaaattac atttcctgtg
     1801 gaagaaattt ttacagtgaa tatgaatagg taactttaac tctgttgtgg atgttatgaa
     1861 tattttgcat agtgcatttc atcagataaa tgcgaagttg cgaatttcga cagctcaata
     1921 gtcgcaagta ggacacaatg gtacacatat tgcacttttg cttattggag ggacgcccat
     1981 gtaaacttct gtaagaagct atgaaaagat ttaagccatt ttggaggctt tgggtaataa
     2041 agaatatttg ggtaataaag aatataaagt atataaattt atagagttta tgcgtaagaa
     2101 ggttatagcc atatatttat agaaaataaa agtagtaata tatctgacaa aacaatagac
     2161 atataaaaaa taagagcata ataaaggaag aattaaaata aataaacgat ttctcgaatt
     2221 gataattctc gtattgataa aaatttccca atatgagaat tttttctttt ataaatctta
     2281 tttttcacat ttaatattta taaaattcaa agaaatttta taaatatatg taattccaac
     2341 ataaaaaaat tgtttttaga ataaataata ttggcatagt aattgcttca ctataagaca
     2401 caaagataaa aaattaaaat gacaccgaaa aatttgaaag gggaatataa aataaatgag
     2461 agaaataaaa tggaaattaa ataacttacc taaatcagag gaaaaagaaa aaggaattga
     2521 gtttttaaga gatgaagaaa ttgcaaaggc taaagctttt catgagagct ttccacaata
     2581 tgaaaaaact cctttagtta aattagataa cttagcaaaa tcactaggcg taagcggaat
     2641 atatgttaaa gacgaatctt atagatttgg tttaaacgca tttaaagttt taggtggttc
     2701 ttactctatg ggtagatact tagctcaaag attagataca gatatatctg agttagggta
     2761 tgacaaatta acttcaaaag aaataaaaga aaaattagga gaaataactt tctttacagc
     2821 cacagatgga aatcatggta gaggagttgc ttggacagct aataaattag gtcaaaaatc
     2881 tgttgtatta atgccaaaag gttcttctga atttagatta aataaaatca aaggagaagg
     2941 tgcagatgct agtataacag acttaaacta tgatgatgca gtaagattag ctaatgatta
     3001 tgcagaagct gatgaccatg gagtaatggt tcaagataca gcatgggacg gatatgaaga
     3061 aataccagca tggataatgc aaggatatgg aactatggct aaagaagcta tagaacaatt
     3121 aaaagaatat ggagtagata gaccaacaca cgtatttgtt caagcaggag ttggttcact
     3181 tgctggtgca gttcaagggt atgttgcttc aatatatgat gaatgcccaa taacagtagt
     3241 agtagaggct gatgaggctg attgttacta taaatcagca gaagctggag atggaaaacc
     3301 aagatttgtt ggaggagata tgccaactat aatggcaggg cttgcttgtg gagaaccaaa
     3361 cactataggt tttgaagtgt taaaaaatta ttcatcagca ttcgtttcag caccagactg
     3421 ggtatctgct aaaggtatga gaatattagg aaaccctcta agaggagatg agaaagtaat
     3481 ctctggagaa tctggagcag ttacaactgg agcattagtt tcaatccttg aaagtgaaga
     3541 tttaaaagac ttaagagaag ctttgaaact agatgagaac tctaaagtat tattaataag
     3601 tacagaagga gatactgacc ctgataaata tagagatata gtttggaatg gtgaatgcca
     3661 aagtaaataa aagacttaag gaaaataaaa acttaacaaa taaatgaaaa atttaaggaa
     3721 aataaaaact taaataagtg aaaactttaa ataaaataaa aactaaaaca aatgaaaaat
     3781 ctaaataaat gaaaaagtaa agcaaatgaa aaattcaaaa taaaaactaa ataaaattaa
     3841 ataaaaccaa ataaaattaa aataaagtct aaataaaaat ttaataaata aaatataaag
     3901 aaataaaaaa tcaatagatt aagtgaaatg ctaataaatt aaaaaagtga aatatcaaaa
     3961 taaaacgtca acaaattaaa aattatatgg gggaatatca aaatgttaaa tgaaacaaga
     4021 aaaaaacaat taactgaagt atgccaagac ttaataagaa atgctagtta ttcaggtcaa
     4081 gaagaaaacg tagtaaaagc tatagaagaa aattttgctt tactaggttt tgatagttgg
     4141 tcaagagata gatatggaaa tatagtagga tgtataaaag gaaataaacc aggtaaaaaa
     4201 atattatttg acgggcatat agatacagtt ccagttccag atgcaagtaa atggagtgtt
     4261 cctccttttg aagcgagaat agtagatggg aaaatatatg gtagaggaac ttctgatatg
     4321 aaggggcaag taagtgctat gatggctgct gtttcatact ttgcagaaga tactaacaaa
     4381 gactttgaag gtgaattgta tgtagcaggt gttgttcatg aagaaatatt tgaaggtgtt
     4441 tcagctagag agataagtaa ggctgttcaa cctgattatg ttgtaatagg tgaatcttca
     4501 gaattaaact taaagatagg tcaaagaggt agaggagaaa tagtagtaga aacttttggt
     4561 aaaccagctc actctgctaa tccagaaaaa ggtataaatg cagtttataa aatggctaat
     4621 gtaatccaaa agatacaaca attagtacct ccaactcatc cagttttagg tgatggaata
     4681 ttagttttaa cagatataaa atcttctcca tatccaggag catcagtagt gccagattat
     4741 tgcaaagcta cttttgatag aagattatta gttggagaaa caagagaagg cgtattagct
     4801 ccaatacaag ctttattaga tgaaatgatg aaagaagacc cagaattaaa tgcaaaagtt
     4861 tcttatgcag ttgaaaaagc tgattgctat acaggaaata ctatagaaag tgaaagattc
     4921 ttcccaggtt ggttatatga tgaagaagat gaatttgttc aagcagcgta taatggatta
     4981 aaagaagctg gaatagattc agaaataact caatactcat tctgtacgaa tggaagtcac
     5041 tacgcaggtg aggctggaat aagaactata ggatttggtc cttctaagga aaatcttgct
     5101 catacaatag atgagtatat agaattagac caattattca aaggtgcaga aggatattat
     5161 ggtatattaa aatcagttta taaaaaataa ttaggagatg gttttgtgag aactttaata
     5221 aaacatgggc ttatagtaga tggaaataaa acaccagcat atgaaggaga tattttaata
     5281 gaaaacgaga aaatattaaa aatatcacaa gatttaaacg aagaagctga caaagttata
     5341 gatgcaaaag gtagagttat atgtccaggt ttcatagata ctcatagcca ttcagaccta
     5401 gttattttag ttaatccata taatgaggta aagattagac agggaattac aacagaagtg
     5461 ttgggccaag atggtatatc tatggcccct ctacctcaag agcatatatc ttcttggaga
     5521 aaaaatttag caggtctaga tggtgaaagt gatgaaattg attggaaata tgaaacaaca
     5581 gagaattatt taaaaatgat ggattacaat ggggtaggtt taaatgaaac ttatttagta
     5641 cctcatggaa acgttagaat ggaagctatg ggacttgaag atagacctgc tactaaagaa
     5701 gaaattcaaa aaatgtgtga aatcacagag agagaattaa aagcaggagc tataggattg
     5761 tctactggac ttatatatat accttgtgca tactcattga ctgaagaaat tatagagatg
     5821 tgtaaggtag tagcaaaata tgacggagta tttgtagttc accaaagaag tgaagcagat
     5881 acaattttaa cttcaatgga agaaatcata gaaattggaa aacaatcagg agtaaaagtt
     5941 catttttcac attttaaggt ttgtggtaaa gcaaactgga agtatatacc acaagttata
     6001 gagcttttag aaaaagcaga aaaggaagga ataagagtat cttttgacca atatccatat
     6061 gcagcaggaa gtacaatgct tggagtagtt ttacctcctt gggcacattc tggaggaact
     6121 gataagttaa tagaaagatt aagcgatgaa aacgaaagag caaaaatgaa aaaagatata
     6181 gcaaatggaa tagaaggctg ggataatttt atagaatttg ctggaataga ccaaatattt
     6241 gtaacaagcg taaaaacaga aaagaataaa gatacaattg gtaagagctt attagaaatt
     6301 ggaaaaatga gaggaaaaga cccattagat gcaacgtttg atttattaaa agaagaagaa
     6361 aatgcagttg ggatggtaga tttctatgga ttagaagaac atataatagg attcatgaaa
     6421 agagacgagc aaaatgtgtg tacagatggt cttttagcag gtaaaccaca tccaagagct
     6481 tatggttctt tccctaaaat actaggaaga tatgttagag agttgaatgt actaacaatt
     6541 gaagaagctg tttataaaat gactaaaaag gcagctacaa gctttagtat aaaagataga
     6601 ggcgaattaa aagaaggata ttttgcagat atagttatat ttgacaagga tacagttagt
     6661 ggttgtgatg attttatcaa ctctatgcag taccctacag gtatagatta cgtaattata
     6721 aatggaaatt gtgttgttga agaaggaaaa tacaataaaa ttaaagcagg aaaagtactt
     6781 aaaaattaaa tcttagcttt aaggaggaat aaatttatga ttttagtggg gaatgggagg
     6841 ctaataagcc aagattcttt aaatccctat atggaagatg gatgtgtagt aataaatgat
     6901 aatctaattg aagatatagg tactacagaa gaaatgaaag ctaaatatcc agtacatgaa
     6961 tttatagatg caggcaagaa aataatcatg ccgggtctaa taaattcaca tatgcatata
     7021 tacagctcat ttgctagagg aatggcagtt ccaggaaaac cttctgagaa ctttttagag
     7081 atactaaata atttatggtg gagacttgac aaacagctta cactggaaga tacaaaatac
     7141 agtgcatatg caacgtatat agaatgcata aaaaatggtg taacaacagt ttatgaccat
     7201 catgcaagtc caaatgcaat tgaaggcagt ttgtttacaa tatcagatgt tgcaaaggat
     7261 ttgggaatta gaacgtgtct ttgttatgaa gtgtctgata gagatggtgg aaatacaatc
     7321 caacaaggta tcaatgaaaa cgtaaacttt ataaaatacg ctcaaaaaga tgatacagac
     7381 atgataaaag gaatgtttgg gcttcatgct gcatttacac tatctgatga tacattaaat
     7441 aaatgtgctt cagagatggc tggtcttgat acaggttacc atgtacatgt tgctgaagga
     7501 ctagcagact tacaatataa tgtagataaa tatggaaaaa gggttttaga aagattcaat
     7561 gattttggag tactaaaaga aaaaacaata gcagttcatt gtatatatat aagtgcagaa
     7621 gaaatcagtc ttttaaataa aacaaataca tttgtagttc ataatccaga atctaatatg
     7681 ggaaatgcag ttggatgttc tcctgcaata gagttattaa aaaggggagt tacagtagga
     7741 ttaggaactg atggatatac tagcgatatg tttgaatcat taaaagttgc caatataatt
     7801 cacaaacatc acttaaaaga cccaagagtt ggatttgaag aatcacaaac aatgttattt
     7861 gataacaata gaaaaatagc taaagcttat tataaaaatg acctaggagt acttaaaaaa
     7921 ggtgcttatg cagatgttat agtagttgat tatataccac atactccatt gacagaaaat
     7981 acaatagggg gacacactat ttttggaatg agtggaagaa gcgtagatac aactattata
     8041 aatggaaaag tacttatgaa agatagaaag ttaataaaca tagatgaaga aagtattcta
     8101 gcaaaaagta gagaattaag tcaaaatcta tggaatagaa tttaaataat tgattatgca
     8161 aaattctatg aagcaggaac cctgtgactt tagtcatggg aggttcagat taatatacaa
     8221 gtaagaaaca agaatttaga tgttaaaaac agagcttaaa caaaaatagg aatttaaatg
     8281 aagtatataa ataaaaaata tacggaatag aatattaaat taaatataga agggagaccc
     8341 aaaatgtctc aagataaaag ccctaaaata gctccagtag atgaaaggtt acctgtatcc
     8401 aaatcttggg tattttcact acagcacgtt ttagcaatgt gtgcaggagc agtagcagtt
     8461 cctatgatga taggaagtgc tgcacgacta gatcatgcta gtattgtttt tttaataaat
     8521 gcttgcttgt ttatggcagg tataggaaca ctcatacaaa gtgttggtat aggaaatatt
     8581 gtaggtgcta aaataccagt aatagaagga actagttttg ccgctgtggc aggtatgaca
     8641 gcaatagcct catcatatag tgattcacaa gttgcaatac agacagtatt tggctctgta
     8701 atagtagctg gattgttttg tatattactt gctccagtat ttgaaaaatt aataagattt
     8761 ttccctagtg tagttactgg aacagttgtt atggtaatag gtatttcact atttccagtt
     8821 gggataaaat ggataactga caacaaattg gaagcggttg aaccaaactc attagcatta
     8881 gctttagcag tattggcaat aactttactt tcatttaaat tcttaaaagg aatttggaat
     8941 agcacagcta tattggtagg tatagtactt ggtacactac tttccatggc agttggaatg
     9001 gcagatttta gtgcagtaaa tggagttggt tggcttaact taaatgtacc tttaaaattt
     9061 ggaatgccta gatttgaacc atctgcaata ctatcattgt gtttgattat gttggtactt
     9121 atgacggaat cagttggtaa tatgatagct attcatgaaa tggtagacaa agaagtaaca
     9181 ggtaaaaata ttaaaagagg tctatttgga gatggaatat ctacagcatt atctggaata
     9241 ttcaattcat ttccaataac tccatttggt caaaatacag gaatagtagg tcttacaggt
     9301 ataaaatcaa gatttatagc agtttacgca ggggttatat tgctagtact tggattctta
     9361 ccaaaatttg cagcaataat aggagcaata cctaagcctg ttctcggagg cgtaggattt
     9421 gcaatgtttg gaatggtaat ggttggagga gtaaaaactt taagcaaagt gaaatttgat
     9481 ggaactaaaa atggggttat agtagcagtt agtgttggtt tgtctatgat accactaaca
     9541 aatcccgatt tctatcataa tttcccaact tgggtacaaa caatattcca tagtggaata
     9601 acaacaggta gtttatcagc agtgctttta aacatattct tcaatgaact aggttctaaa
     9661 aaagaaaaaa ataataaagt tgaagaagca aaataaaaat aaaacaagta aaaatctata
     9721 ttgacttaaa gtcattaagc attaataaaa ataattcaga gatattaata attcaacaaa
     9781 actatcttat attttaaata atataaaaaa gtaaataaat aacgaataca aataaaagaa
     9841 tacaatctaa atattaaaaa tatttaattt aagggagaga taataatatg gctaatttaa
     9901 aagttaattt atatggaatg gaatttttaa acccaattat gacagcggca ggacctggag
     9961 caaaagatgg tgctctctgt caagaggcag caaaaggtgg agcaggagct atatgtacaa
    10021 aaactatatc tgtattacca gctgatgtac caagaccatg tatggcaaaa actaatagtg
    10081 gttttttaaa cacagaatta tggtcagagc ttccaaaaga gcaatggata gaggaagaat
    10141 acaaaaaggt aaaagaaaca gggcttccag taatagtaag tatgggatat tcagcagagc
    10201 aaatagctga ggttgctcct ttagtaaagc cttatgcaga tgcagttgaa ttatctactc
    10261 attatgtagg tacagacgtt gcaccaatag taaatgcttt aaaagctgca aaagctgttt
    10321 tagatgtacc agtatttatg aaaatgagtc ctcatactga cattcaaact attgcaaaag
    10381 ctgttgaaga agcaggtgca gatggacttg taatgataaa ctcatttgga ccatgtatgg
    10441 caatagatgt aaatacagga taccctataa tgggaagtaa agctggatac ggatggttat
    10501 caggtgcacc aataagacct ctagcaataa gatgtatata tgaagcttct caagttgtaa
    10561 atataccaat aatcggtgtt ggtggaatca cttgtggaaa agacgtggct gaaatgatga
    10621 tggctggagc aagtgctgtt caagtatgta cagaagcaat ccttaaaggg ccttctatct
    10681 atggaaaaat tgctaaagaa ttaaatgaat ttttagatga gaatggatat gctgatgtta
    10741 atgaaataaa aggattaact cataagaaaa tggcagctag agggtttaga actcattcag
    10801 tagctccagt agtggatgat aacaagtgca tcaaatgtaa gcaatgtgta acaagctgtg
    10861 tatacgaagc tttagaagtt acagataagt tgaacataga tgcagacaag tgttttggat
    10921 gtggattatg tgtaactaga tgtccaaaag gagctttaag catctgctac aatcaagtag
    10981 aagctatgaa tgagtaataa ttatatagaa ttttggtaat taacttagag taataggtat
    11041 taggataata aaatatatca gataaacaaa cacacattat gcattaataa tacagtctaa
    11101 agttaataat tttattatat tgatttaact aaatattagt ttagctgaat attaatttaa
    11161 ttaggtattg gtttaatctt aatatagatt tactacgtat aagtgaacta actagattac
    11221 tttaagttaa ttttttcata taaaataaat tgatacatta caagtaaggt ggagtctata
    11281 tgggaattgt tttaaaaggt gggaaagtag taacaagtga aaaaacttac tgtgcagatg
    11341 taagaataga agatgaaaaa atagtcgata taggtcttga tatatcaaaa gaaaatgatg
    11401 aaatagttga tgttagtgat tcttatgtta taccaggagg tatagataca cacacacact
    11461 ttgacttaga tacaggcgtt actaagacag cagataactt tgagacagga acaaaagctg
    11521 ctatagtagg aggaactact actatattgg attttgctac acaagctaaa ggatgttctt
    11581 tgaaagacgg attagaagaa tggcatgaaa aagctaagga taaatcctat tcagattatg
    11641 gctttcatat ggctataaca gattggaatg actcggtttg taatgaaatg gaggatatgg
    11701 tgaaagaggg agtatcttca tttaaactgt atatggcgta taaaggaagc cttcaagttg
    11761 atgatggtgt aatctttgaa gcattgagaa aagcagaaga gataggaggg attattggtt
    11821 tccattgtga aaatggggac ataatatgtg aacttgtgga taaagcaaag tcagaaaata
    11881 atttatctcc aaaatatcat caattaacta gaccagctat aatggaggca gaagctacat
    11941 caagacttat gaagatagct gaagttgcaa actcaaaagt atatgtagtt cacttaagct
    12001 gtaaagaatc cttagaggag gttttaaaag ctaagcaaag aggtgtggat gtaatagttg
    12061 aaacttgtcc tcaatacctt ctattaaatg atgaattata tgatttagat ggatttgaaa
    12121 gtgctaagta tgtaatgtca ccacctttaa gaaataaaga aaataatgaa atattatgga
    12181 atgctatttt agataatcaa atagacacta taggaacaga ccattgttct tttaattata
    12241 agggacagaa ggaacttggg ataaacgatt tcagtaagat accaaatggt tctccaggtg
    12301 tagaacatag gatgagtctt ttatacacat atggagtctg taaaaataaa atatctatca
    12361 acaagatggt agaagtaaca tcaactaatg cagcaaagtt gtttggaatg tatcctaata
    12421 aaggaactat agaaataggc agtgatgcag atatattagt cttaaatcca aacaaaaaat
    12481 ccaaaataga agcaaaaaaa caattacaaa atgtagatta tacaccttat gaagggtatg
    12541 aagtggattg tcaaatagaa catgtttatt taagaggaaa agaagtagtt agagatggag
    12601 aattaatttg taatactcca agtgggagat atataaatag gcgtctaaca aagggggcta
    12661 gagaatggta gaatttatat taaatggaga gaaagttgtt gcaaaagaag gcaaaaaact
    12721 tttagacttc cttagagaag atatgaatat aacttcagtt aaaaatggct gttcagaagg
    12781 tgcttgtgga acatgtatgg ttataataga tggtaaagct actaaagcat gtgtatttaa
    12841 aactgataaa ttagctggga aaaacataac aactatagag ggattatcag agagagaaag
    12901 agatgttttt gcatattctt ttgctagtca aggagctgta caatgtggat tttgtattcc
    12961 aggtatggta attagttcaa aagcacttat agatgttaat aataatccta ctgaagatga
    13021 tataaagaaa gctttaaaag gtaatatatg tagatgtaca gggtatgtaa aaataattaa
    13081 agctgtcgat ttagcagcaa agatttttag agaaaatata gatgtgccaa aagtaaactg
    13141 taagggtcta gtaggtgaaa atcttcctag agttgatgca gttggcaaag cacttggata
    13201 ttctgaatat gtagatgata tgagaattga gggtatgatt tatggttcag cagttagagc
    13261 taagtatcct agagctttag ttaaaaaaat agatatagaa gaagcaaaag cttatccagg
    13321 agttgttgca gttctaacag ctaaagatat tccaggaact ataaaggtag ggcacttaaa
    13381 gaaggattgg gatactttaa taccagaagg tgaaataact agatatttag gagatggaat
    13441 agttttagta gcagcagaaa ctagagaagc gttagaagag gccaaaaagc tagttaagat
    13501 agattatgag gaattaacac cattaagtac tacagcagat gcacttagag aagatgctcc
    13561 aaagatacat gagacaggga atatacttgt taaggaacat ttagtaagag gaaacgctaa
    13621 agaaaagata gaaaattcaa aatatgttgt aactaaaaga tatactacac ctgctacaga
    13681 acacgcattt ttagagcctg aatgtgcagt agctggtcct tatgaagatg atggagtaat
    13741 aatctattca acagaccaag gggtgtttgc cactcaacat gaatgtgcag atatgttagg
    13801 gttaccatat gagaaggtaa gagttataaa caaaatggta ggaggagggt ttggtggaaa
    13861 agaagacatg agtgttcaac accatgcagc cttacttgca taccatacaa agagatttgt
    13921 aaaagtacag ttaacaagaa aagaaagtat tattgttcat ccaaaacgac atgccacaga
    13981 aatggaattt acgacagcat gtgatgaaaa tggatactta acgggtatga aggcaacgat
    14041 aatatctgac acaggtgcat atgcctctct tggaggacca gtgctacaaa gactgtgtac
    14101 tcatgcagca ggaccataca attatcaaga tattgatata acaggaacag cagtatatac
    14161 taataaccct cctggaggag catttagagg atttggagtt acacagagtt gttttgcaac
    14221 tgaatgtaat ctaaatttac ttgctgaaat ggttggaata tctccttggg agataagata
    14281 taaaaatgca ataagacctg ggcaagtttt accaaatggt caatttgcag atgaaggaac
    14341 agcaatggca gagacactag aagctgtaaa agaagagtat gaaaagcatc cacgtgcagg
    14401 tatagcttgt gctatgaaaa atagtggggt tggagttggt atacctgatg ttggaagatg
    14461 tagattggtg ataaaagatg gcaaagttca cataagaaca agtgcttctt gtataggtca
    14521 agggcttggt actgttatga ctcaagtagt ttgtgaaact gctagcctac ttcctgaaaa
    14581 tatagtgcat gaagttccag atactcattt aacaccagac tctggtaata ctacggcttc
    14641 aagacagaca gtatttacgg gagaagctac taaggttgca tcaaataagt taaaagaagc
    14701 tttagactct ggcaaaactt tacttgattt agagggagaa gagttttatg gagaatatga
    14761 aagtattaca gataaaatgg gttgtgacaa agaaaatcca gtaagtcatg ttgcttatgg
    14821 atatgcaaca caagttgtaa ttctggatga aaatggaaaa ctagaaaaag tagtagcagc
    14881 acatgatgtt ggaagagcta taaatccaat cagtttagaa ggtcaaatag aaggtggagt
    14941 agttatgggt cttggatatg ctctaaccga agattaccca gtagttaact ctgttcctac
    15001 agctaagttt ggtacattag gtttatttaa atctacagat ataccagaaa tacaagcttt
    15061 aatagttgaa aagaacaaag atttattggc ttatggggca aaaggtgtag gagaaatttg
    15121 tacagttcct actgcaccag cagtacaaaa tgcctattat gtatttgata aagaatttag
    15181 aactgagcta ccactacaaa atactccata tagtaaaaaa aagtaacaaa aaaaagaaat
    15241 attaaataaa aaaatagatg aaattgtaaa taaaaaaaac ccttttgcat aaggtatatt
    15301 ttaaatgata gaatatacat atacaggagg gtttttcttt tataaaataa gtttaataaa
    15361 attgagaggt gagcagaatg aattttaata atagtctaga attaactcag tctcagaagc
    15421 taataatgac tacacaactt aagcaatcat tatcaatatt aaatatgagt aagttagagt
    15481 tagaagaaga gataaaaaga gaagcagaga ataacccatt agttgaagtc gagaaaagtg
    15541 gggagataaa ttgggaagag tatataaaag acatggatag gtcaagacgt ttagatagaa
    15601 cagagataag ctataatcct gataatgaag ttaatctaga aaatttagtt aaatattcgt
    15661 caaatttata tgaagattta aaatttcaga taagcctgta taaacttaca gacaaagaat
    15721 tggaagtctg tgagtatata attgatagtt tagatgagga tggatattta agaactgagg
    15781 aaaaagtaat tgttgatacc ttaaagatag atgaagaact ttttgaaaaa tgtttaatta
    15841 gtgttcagca gttggaacct agtggagtag gggcaagaag tctttcagaa tgtctgatga
    15901 tacaaatggc tagtttagga atatataatg aaattttaga ggaaatcgtt acaaaagact
    15961 taaatttaat tgcaaataat aaatataaag agataagtaa aaagtacaat atgtcattac
    16021 aaaaatgtgt tgaactaata aacataatta agacattaga ccccaaacca ggaagaactt
    16081 gttcagtaga gaaaagtgta tacattcaac ctgatgtaac agtagaaaag attgatgatg
    16141 aatttattgt atatttaaat gagaaagatt cttaccaaat aagaatcaat agttattata
    16201 aagacatatt aaaaaattca cagtctgatg aaagtgcaaa agaatttata aaagaaagac
    16261 taaattcagc aacaggactt atgaaaaata tagagagtag gaaaactaca gtacttaaga
    16321 ttgcagaaga gatagtaaaa gctcaagatg aatttttgag aaaagggaca aaatatataa
    16381 aacctttgaa aatgaaagac atagcagaaa aattagaatt tcatgaatct actataagta
    16441 gaggtgtaaa tggtaaatat atgctaacac cttttggcgt atatgaattt aggtactttt
    16501 tcagtagcgc cattgagact gagaataatg aaatggcatc tagtacaagc ataaaaaaaa
    16561 ttataaagga aacaataaaa gacgaaaata aaaagaaacc tttaagtgat gaccatatat
    16621 ctaagttatt aaaagaaaaa ggaatcaatg ttgcaagaag gacagttgcc aaatatcgtg
    16681 aagaattagg aatcccatct tcaagtaaga gaaaagaata ctaataagta aaagacaaat
    16741 atcagtatgg taaggggaaa aggctaatta agagatgaat gtagattggg ctaaaaattt
    16801 tataaagtaa aataatagaa tatggaaaag tattaaaatg ctataaggta ttaaaaaact
    16861 ggggaacatt aaaacattaa gatagcaaag aacataaaaa actaaagaag attaaaacat
    16921 taaagaacat taggctttga ggaatattag aactttaaag aatattaaaa tttataaaat
    16981 ataaaaatac taaaaagaat tgaaaaactg gggaacatta aaatattgta aaatgctaaa
    17041 aaactagaaa acataattaa ataagtgctt ataaagaatg attaaattaa ataagtgttt
    17101 gtaaagaata attagatatt agaaaaagaa gaatacctag ataaataaac tattattaaa
    17161 tttaataatc acaaaaggaa aacacgtagg gtataaataa aaatcttaac tagataaaag
    17221 taaacttagt tgtagataaa aataactaga aataaaagta actagaaata aaattattta
    17281 tagtaataaa tattagaatg aaaatataaa aaaatatata taaaaagcct tatataaaaa
    17341 gaaaaaatac tgtgatttga aaatggtgga gaaatatttt ataaaataaa aaaataaaaa
    17401 agtttttgaa aaactattga aatctatacg caaatcctat aaaataaaaa tatcttgtgg
    17461 gacttaaata aacactaggg acaaaaaaag tcccgaaagg aaagcaataa ggagtatgtt
    17521 atgaagaact tattagaaat tcaaaagaaa ttgattccac aggcaattga acttatggaa
    17581 agaagatact ctattttaag acaaatatct ttaagtgaac caataggtag gagaacatta
    17641 tcaaatgtat tagagattag tgaaagaata atcaggtcag aaactgagtt tttaaaagaa
    17701 caaggactta tagacgtagc tgtatctggg atgactatca ctaaagaagg ttctgatttg
    17761 ttagataaac ttaaggacat catgagtgac atcatgggat tgtctaagtt acaagataga
    17821 gtcagagaaa agcttggaat aaaaagggta atactagttc ctggaagctg tgatgaaaat
    17881 ggaagtttac taaaagatgt aactagatat ggatgcgagt atttcttagg cattttaaaa
    17941 gatggagata tcgtttcaat aacaggagga agcactatgt tggagttttc aaacgttatt
    18001 aaaactgata agagatatcc aaactctaca atagttccag ctagaggaag tatgggaact
    18061 gatgtagaga tgcaatctaa caatatagtt gcttcaataa gtaagaaatt acattctaat
    18121 tgcaaacttt tacatatacc agatgagttg gatgaaagtg caatgaaaac tcttagccaa
    18181 gtgccagaga ttaagacaac tttggagtat attaaaagaa cagacatttt agtttttagt
    18241 ataggtagag cagatgtcat ggcaaagaga agaaaacttc cagaagacaa ggttgatgag
    18301 atattatcta aaaatgcagt tggagaagca tttggatatt atttcaacaa agatggagaa
    18361 atagtttaca aactgaacac tgttggaatt gatttagaaa cagttaaaca agtaaaagaa
    18421 actattgcaa tctttgcagg gactgaaaaa gtagaagcct tattagctat atcaaatatg
    18481 cataaaaaca tggttcttgt aacagatgaa gaaagtgctt ataagatatt gtcattatta
    18541 taaaagtcaa gacctagcac agagctagaa aacatacatt tactttatag gaggtagcga
    18601 aaatggttaa agtagctata aatggatttg gaagaatagg aagattagca ttaagaaaaa
    18661 tgatggagca acaagataag ttcgaagttg ttgcaataaa cgacttaaca gacgctaaaa
    18721 tgttagctca tttatttaaa tatgatacag ctcaaggaag atttaacgga gaaatagaag
    18781 ttaaagaagg tgcttttgta gtaaacggaa aagaaataaa agtaacagca gaaagaaatc
    18841 ctgctgacct accatgggct gaattaggag tagatatagt attagaatgt acaggattct
    18901 ttacatctaa agataaagct gaagctcata tacaagcagg ggctaagaaa gttgttatat
    18961 ctgcaccagc tactggagat ttaaaaacaa tagtattcaa cacaaacagt gacatattag
    19021 atggttctga aacagttata tcaggagctt cttgtactac taactgttta gctccaatgg
    19081 ctaaagtatt aaatgataaa tacggtatag aaaaaggatt aatgactact atacatgcat
    19141 acactaatga ccaaaatact ttagatggtc ctcatccaaa aggtgattta agaagagcta
    19201 gagctgctgc tggaaacata gttccaaaca ctacaggagc tgcaaaagca ataggcttag
    19261 taataccttc attaaaagga aaattagatg gggcagcaca aagagtacca gttgtaactg
    19321 gttcaataac tgaattagtt tgtacattag gaaaaaatgt tacagttgaa gaaataaatg
    19381 cagctatgaa agaagcttca aacgaatctt tcggatatac tgaagaaatg ttagtatctt
    19441 ctgacataat cggaataagc tacggttcat tatttgacgc tactcaaaca aaagttatgg
    19501 aagtagatgg aaaacaatta gttaaagttg tttcttggta tgataatgaa atgtcttata
    19561 cttctcaatt aataagaact ttaggatact ttgcacaatt agcaaaatag tcagttttga
    19621 gaaaatagaa aagtagattg acaaagtccg agtttgtagt ttaatactac ggactcagga
    19681 ctttttttaa gaaaaaatat ataaaataca cctaaataat gttacatact atatataaaa
    19741 atatgataat atgatatatt taatagggta aaagtatgta ctatattata cttggcaata
    19801 aaaaattact ttattaaaag gagagatatt tatgtcaatg cttaacaaaa aaactataga
    19861 agatattgat gtgtgcggaa aaaaagtatt agtaagatgt gactttaatg tgccattaca
    19921 agatggtgta attactgatg aaaatagatt gaacggagct ttaccaacta ttcaatattt
    19981 aatttctaaa ggggcaaaag ttatattatg ctctcactta ggaaagccaa aaggagaagc
    20041 taaaccagaa ttatctttag caccagtagc aaagagatta tctgagatgt taggaaaaga
    20101 agttgtattt gcagcagacg ataatgttgt aggagaaaat gcaaagaaag caactgaaaa
    20161 aatggaaaac ggagatgtag tattattaga aaatactaga tatagaaaag aagaaactaa
    20221 aaacgaagag aatttctcta aagaactagc atcacttgct gaaatatttg taaatgatgc
    20281 atttggaact gctcatagag cacattgctc aacagttggt gcaggcgagt ttttacaaga
    20341 gagagtttgt ggatacttaa ttcaaaaaga attaaaattc ttaggagaag ctgttgcaaa
    20401 ccctgtaaga ccatttacag ctatacttgg aggagctaaa gtttctgata aattagctgt
    20461 tataaacgaa ttattagaaa aagttgataa tctaataata ggtggaggaa tggcttatac
    20521 attcttaaaa gctcagggtt atgaagtagg aacttcttta cttgaaatag ataaagttga
    20581 atatgctaaa gaaatgatgg aaaaagctaa taataagggt gttaacttat tattaccagt
    20641 agacgttgtt atggcagacc actttgcacc agatgctact ccaatagtaa cagaagatgc
    20701 taatgtaaaa gaagattaca tgggactaga tatgggacct aagactatag caaactttgt
    20761 aaaaactata aaagaatcaa aaactgtagt atggaatggg ccaatgggag tatttgaatt
    20821 tgaaaacttt gcaaatggta cattatcagt agctagagct atggcagagt tgactgatgc
    20881 aactactgta attggcggtg gagatagtgc agcagcagtt aatcaattag gatttggtga
    20941 caagatgact cacgtatcaa caggtggagg agcatcacta gaatttttag aaggtaaaga
    21001 attaccaggt attgcagcat tagataataa ataaactaat atatagagga gattgtaaaa
    21061 tgagaaaacc tataattgca ggaaactgga aaatgcataa aacaataaaa gaagctttag
    21121 aatttgtaaa tgaagttaaa gataaagtaa attctgataa agtagaagct gttatatgtg
    21181 caccatttac tttattaaag gatttaaaag aagctactaa gggtacaaat ataaagatag
    21241 gtgctcaaaa tatgcacttt gaagaaaaag gagcatttac aggagaagtt tcacctctta
    21301 tgttaaaaga aatagatatg gactatgttg taataggaca ttcagaaaga agacaatact
    21361 tcaatgaaac tgatgaaact gtaaacaaga aagttttaaa agcattagaa gtaggaatag
    21421 acccaatatt atgtgttgga gaaactttag aacaaagaga agctggaaaa actaaagatg
    21481 tttgtaaagt tcaagttgaa aaagctttag aaaatgttct taaagatgat ttatcaaaag
    21541 tagttgttgc atatgagcca atctgggcta ttggaactgg taaaactgca actgctgaag
    21601 atgctaatga cgttatatct tatataagag aagttataaa aggattatat ggagaattag
    21661 ctaatgaagt tagaatacaa tacggtggaa gtgttaaacc ttcaaatgta gccgaaataa
    21721 tgggtcaaag tgatatagat ggagctttag taggtggagc tagtttagct agtaatgatt
    21781 acctagacct tgttaatttc taaggagtga aatgatgaaa aaaccagttg ctttaattat
    21841 tatggacggg tttggataca ataaggatgt taagggaaat gcaattgctg agtccaaaac
    21901 tcctaattta gatagaataa aaaaagagta tccaaacaca ttgataaatg ctagtggtct
    21961 tgatgttggt ctacctgatg gtcaaatggg aaattcagaa gttggacaca ctaacatagg
    22021 tgcaggtaga atagtatatc aagatttaac tagaataaca aaatctataa aagatggtga
    22081 cttttttgct aataaagttt tatgtgaagc aatggataat gcaaaagaaa attctcttca
    22141 tgtaatggga cttttgtctg atggaggagt acattcacat atagaccatc ttaaggctat
    22201 aatcaaaatg gcaaaagata aaggcgtaca aaaggtgtat gttcacgcat ttactgatgg
    22261 tagagatact gaccctcaaa gtgcattaga atatgccaaa gaagtacaag ctagtatgga
    22321 tgaaattgga gtaggtgaat ttgctacagt ttctggtaga tactacgcta tggatagaga
    22381 taagagatgg gaaagagtag aacttgcata caatgccatg gttagaggta ttggtgaaaa
    22441 agcaaattct attgaggaag ctattcaaaa ctcttatgat gatggaaaaa atgatgagtt
    22501 tataatgcca acagttatta tgaaagatga taaaccagtt ggaagtataa aggaaaatga
    22561 ttcaattata ttcttcaact ttagaccaga tagagctaga caaataacta gagctttagt
    22621 ttgtgaagag tttgatggat ttaaaagaga agatattaaa aacttctttg tatgcttaac
    22681 agagtacgat ataactatag agaatgtgca tatagcattt ggacctcaat ctttagccaa
    22741 cactttaggt gagtatctag ctaaaaatgg aaaaactcaa cttagagctg ctgaaactga
    22801 aaagtatgca catgtaactt tcttcttcaa tggtggagta gaagagccaa ataaaggtga
    22861 agaaagacta ttgatacctt ctccaaaggt tgcaacttat gatttaaaac cagaaatgtc
    22921 agcatatgaa ttgacagata aagctttaga taaattagga gaagataagt ttgactttat
    22981 agtattaaac tttgcaaatc ctgatatggt aggtcacaca ggaagtatag aagcggctat
    23041 aaaggctgtt gaaactgtag atacttgtgt aggaaaattg atagataaaa tagtagagtt
    23101 aggtggaagt gcaataataa ctgctgacca cggaaatgct gaatatatgt tagacccgga
    23161 aacaggaaaa actgtaacgg ctcactctat taatccagta ccatttattg tagttggtca
    23221 agaatatgaa agtgcaaaat tattggatgg aggaagatta tctgatatag caccgactgt
    23281 tttagatatg atgaagttag aaaagccaga agaaatgact ggtcattcac ttatttcaaa
    23341 atagtcgatt ctgataagta gagaataata tataaactac aatataaatt taaaaataat
    23401 gcagtttagg tttattttgt aaagtttaga ctttatgaca taagatttag attttgtaag
    23461 ttctggatat tgtaaggtgt agatgaaggt atattttata aaatttacaa gatagacaat
    23521 ttataaaata aaaaattata atatagataa taatgtaaat aatgatataa ataataacaa
    23581 gatagac
//
LOCUS       DAELXX010000061        23276 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.061, whole genome shotgun sequence.
ACCESSION   DAELXX010000061 DAELXX010000000
VERSION     DAELXX010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23276
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.061"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            423..614
                     /locus_tag="KPH29_002215"
     CDS             423..614
                     /locus_tag="KPH29_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467804.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
     gene            complement(703..1515)
                     /locus_tag="KPH29_002216"
     CDS             complement(703..1515)
                     /locus_tag="KPH29_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HBG1467805.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVVEKIK"
     gene            1937..3088
                     /locus_tag="KPH29_002217"
     CDS             1937..3088
                     /locus_tag="KPH29_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG1467806.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVVSDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDDDLDKMLELYNQNSTRFIRTRDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLEYLNYKVHVKNLKDQLKCNG
                     TESLDYQQGTMKIINFTKLCDSLRSYFSQYVDFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
     gene            3206..3844
                     /locus_tag="KPH29_002218"
     CDS             3206..3844
                     /locus_tag="KPH29_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBG1467807.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            3845..4867
                     /locus_tag="KPH29_002219"
     CDS             3845..4867
                     /locus_tag="KPH29_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HBG1467808.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLETLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEYIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLSCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRGYDNFLCDFGCNCLNTTPVENLQAAIKAGRINYKELDR
                     IDIDEL"
     gene            4857..5477
                     /locus_tag="KPH29_002220"
     CDS             4857..5477
                     /locus_tag="KPH29_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467809.1"
                     /translation="MNYNSNKNKYLLKDINLEDIFIDKNIVLKFLGYGNRKAPDTISE
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDIKMNDYMENCNM
                     MAGIALDKIGIVCLDYINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(5601..6287)
                     /locus_tag="KPH29_002221"
     CDS             complement(5601..6287)
                     /locus_tag="KPH29_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBG1467810.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQKKRLENSKIKGYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALMIGNSLTSDVLGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(6301..6846)
                     /locus_tag="KPH29_002222"
     CDS             complement(6301..6846)
                     /locus_tag="KPH29_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1467811.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCIDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            7074..8321
                     /locus_tag="KPH29_002223"
     CDS             7074..8321
                     /locus_tag="KPH29_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG1467812.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLAFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSSGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            8408..10402
                     /locus_tag="KPH29_002224"
     CDS             8408..10402
                     /locus_tag="KPH29_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG1467813.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDAVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLISMIKGVIKNTFSENTN
                     KTSEIEIYNKILKINTFPVEYKKNNTEYIVVLIKDITNEKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIRNFIENIVKLQYKMLQLKNVEIKIDCEHNLVCYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNHCLFIDFKDNGEGIKEDALKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            10402..11724
                     /locus_tag="KPH29_002225"
     CDS             10402..11724
                     /locus_tag="KPH29_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBG1467814.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNFEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
     gene            12003..13289
                     /locus_tag="KPH29_002226"
     CDS             12003..13289
                     /locus_tag="KPH29_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBG1467815.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            13375..14682
                     /gene="grdB"
                     /locus_tag="KPH29_002227"
     CDS             13375..14682
                     /gene="grdB"
                     /locus_tag="KPH29_002227"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:14422..14424,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBG1467816.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVVTGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     VGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE"
     gene            complement(14898..15122)
                     /locus_tag="KPH29_002228"
     CDS             complement(14898..15122)
                     /locus_tag="KPH29_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG1467817.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(15219..16472)
                     /locus_tag="KPH29_002229"
     CDS             complement(15219..16472)
                     /locus_tag="KPH29_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HBG1467818.1"
                     /translation="MITLSFDIVQTLTLSIFFFLIGNLLKNKVNFLNNFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNIGLSIPRYNRNLKRFGSKFFRNK
                     NRIKNNNFSETITPSVFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVI
                     FRNLNDKINILELNFKLIDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGDSPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
     gene            16732..17721
                     /locus_tag="KPH29_002230"
     CDS             16732..17721
                     /locus_tag="KPH29_002230"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBG1467819.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEVHPFYTFYKDNLHVSINT
                     DNRTVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            17793..18533
                     /locus_tag="KPH29_002231"
     CDS             17793..18533
                     /locus_tag="KPH29_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HBG1467820.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPPLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            18733..19884
                     /locus_tag="KPH29_002232"
     CDS             18733..19884
                     /locus_tag="KPH29_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBG1467821.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYALGKLEPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            19909..20664
                     /locus_tag="KPH29_002233"
     CDS             19909..20664
                     /locus_tag="KPH29_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HBG1467822.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKNTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNKNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            20856..23243
                     /locus_tag="KPH29_002234"
     CDS             20856..23243
                     /locus_tag="KPH29_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HBG1467823.1"
                     /translation="MKKFTSKKVARFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SDTYKQYLNDAKNGRTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSVKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMDGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVSYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFNIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
BASE COUNT         9300 a         2494 c         3960 g         7522 t
ORIGIN      
        1 ttagtacagt aacgtctaaa atagacaagt ctttttttaa tagatacttt ttgttttttt
       61 caactctaac tttatttaaa aaattagtaa aagtatatcc actttttttc ttaaacaaag
      121 aaaaaaataa cttttattaa gttgtaattc attacatata ttacttactg ttatatcttc
      181 tgagtagttt ttgtgaatat aatctatagt tttctttaag ttttctttac ataaaactaa
      241 gagttggtct atgtttcatt ttatcaaggc atatttatta aaaaatcaat ctctatttgt
      301 taaaataatt tatgaatgat gttagaaact gtaatatatt ggatataata taataaacag
      361 ataaactctg tattaagagg atatatagtt tatcttaatt ataggggaag tgaggatata
      421 ttatggaaag attaatggaa ctcatagaaa gaaaattttt aaaggatgta gaacttttag
      481 aattagaaga catagaaaat gtaatagaat gcaaaatgac acagccatat aatgctcatt
      541 ataatgaata cttagtaaaa gtaaaaaatg gagatgaagt agaagaatat tttgtgtatt
      601 taaaacatag ataaacataa aagaggttta tatttatttg tatgattata aaataaatac
      661 aagcctcttt ttttgataaa tataaaatta taaaattttt atttatttaa ttttttcaac
      721 aacattagtc aatattgaat ctaaatcatc actaatttct gtaactccat tatgagtaat
      781 atctataatt tctttagtca taggaagttc tcctaaaagc tctaagtcta aatcatctaa
      841 aaacttttca gtagattctc cttcaaataa cttgattttt ttagaacaat caggacattg
      901 gatataactc atattttcaa taactccaag gacatttatg ttcatttttt tagccatatt
      961 aacagctttt gatacaatca tagataccaa atcttgaggt actgaaacca taactattcc
     1021 acttatagga atagattgca taaccgttaa ggcaacatct ccagtaccag gaggcatatc
     1081 tattaataaa tagtctaatt cttcccaaag tacatcagta taaaattgct ttacaactcc
     1141 tccaagtaaa ggtcctctcc aaacaacagg ttcattctca tcatctatca taaggtttat
     1201 agacattact tttatgttat ttgagttttc tacagggtaa atataagagc catcagaata
     1261 agcttttaca ttttttacac ccataagtct aggaatactt ggtccagtta tatctgaatc
     1321 taatatacca accttatatc ccattttgtt tagcttatta gcaagtaaag cagttacagt
     1381 tgattttcca actccaccct tcccactcat aacaccaatt atttttccaa atttattgtt
     1441 agggttattt tcaatagaac aatttgattg actattacaa tttccctttg atggacatga
     1501 attacaatct gccatatcta ttacctccaa actttaatat ttattagatt atttgtaacc
     1561 tacatatact attttagtac aattgaacca taaagtcatt atttaaaagc aacttagaag
     1621 attttctatt gatttattca tagactatag caaaaagaat acaataaaaa aattataaca
     1681 ttcttgaaaa agtgattatt tcaatttagt tatagaactt agtcatataa atatatagaa
     1741 aatattttaa tatattatac ataacgggtt attttataag tttaactata aattaggttt
     1801 atattttata aaaatttgtt aaaaaaaaca attaaaaatt gtaaagaaaa aagatataat
     1861 tagaactgta gtaataaatg ttttattata gataatagag ttttaagtat cttaaataat
     1921 atttggagga atatgaatgg aattaagacc aagaagtgca aaattagaag aatttgataa
     1981 agtaatagaa ttaataaact atgtatttag aatatcaaga aatcataagc caactatgat
     2041 ggaagaattt cctcttttac taagtaaaaa taatatagaa aatatgatta taatatcaga
     2101 agatgataaa gtggtttctg atgtaaacta tctaattcaa gatgtatcta ttcaaggaaa
     2161 tagattgaag gttgcagcta taggtggagt atgtactcat ccagattatg aaaagagagg
     2221 ctattcatct aagatattgg ataaagtaga agaaaaaatg ttttatgatg gtgtagatat
     2281 tgtaattata tcaggaacaa gaagtctata cagtagaaga aattgtagtt tggtaaagag
     2341 tttttataaa tatacaataa aacctgaaga tgtaaaaata gcatatgaaa tagttgaatt
     2401 tgatgaaact aattttgaaa aagatgatga tttagataaa atgcttgaac tatataatca
     2461 aaatagtact agatttataa gaacgagaga tgagtttcaa aaactattgc atgcagcaac
     2521 tattgcctgg ggaccaattg gttacaagaa ggtatttata aaggaaaata ataatataat
     2581 aggatatttg ataataagaa caataaaaaa agaagattct actgtaggag aagttgctga
     2641 aatagggtta aactctgtaa atgttgaaaa tatattgaaa tatgttgcaa ataaatttgg
     2701 attagaatat ttaaattata aagtacatgt gaaaaatctt aaagaccagt tgaaatgtaa
     2761 tgggacagaa tctttggact atcaacaagg aactatgaaa ataataaatt ttactaagtt
     2821 gtgtgatagt ttaagaagtt attttagtca atatgtagat tttgaattac taaaatacat
     2881 ggaatttaaa caagttgaaa ataaatatat tattaaatac aaagaagaag aacttgtaat
     2941 agaaaatttg gataagctta ataaactgtt ttttgaaaaa aatgaggaac agtataatga
     3001 atttaaacat ctaaaaaata tatatgaatt tgctacaaag gcatttcctg tagattttcc
     3061 atggactgca aatttaaatt atcaatagtt ttagatgata ttaaataaaa ttgattgata
     3121 cctagagtta gtccttgtta aataagagaa attattggat attgaaaaag caattttgta
     3181 agtaattaga aaggatgagt attatatgga catacttgat gatatagcag aatgtatatt
     3241 aaatatgggt gtagataata tagagaattt agtaaaaatt gctatagata aaaatattga
     3301 tgtggaagat atttatgaat atggtttaaa caaaggtatg ataggagctt tagataagtt
     3361 tgagaataag gaatactatc tttcagaagt tatagtatgc acagatgcat taaataaagg
     3421 tattaatctt ttgaagggaa ctggaaaagt aaagaaaaaa tcaaagggag taattctgat
     3481 gtctgtagta gaaggagata ctcatgagat aggaaaaaat attgtaaaag taatggtaga
     3541 agccactgga tataaggtta ttgacttagg tgtaaataga aaaagtgaag atataataga
     3601 agaagctata aaaaacaatg tagatataat aggcttatct tcaatgatga caactacaat
     3661 ggaaaatatg aaatcggtta tagacgagct taatatgatt gagatagata aaagaccaaa
     3721 agtaataatt ggtggaggac ctgtaagtat ggagtttgca gaagaaatag gagcagatgg
     3781 atacagttca aatgctccaa aagcagttaa attaataaac aaacttatag gaggtgaagt
     3841 ttaaatgttt tcagatatga ctttacttca gtatataaat agtgaagata gaggtttttt
     3901 tctgcctgat atgggtacaa atggattgtt cttaataaat aaaaaggcat atgaagtata
     3961 tgatagtgta gatttgcaat tagaattagc aaagactatg gacaagtatt ttgaatcaga
     4021 ttttatatac tcattctgtg atgggattac attttgtgaa actctgggtt tagagacatt
     4081 aagaccagat tatgattttc caagtgtatt gacacatact ataactgaca gagataaatt
     4141 aagaaaatta gatgttccag acccttatac gagtggacgt atgccactca atatagagag
     4201 cttatcagtt atatcaaaaa gtattaaaaa accactatat gaatcgatac aaggtccatt
     4261 tacacttgca ggtcaattag ctggagctac tcaactttta agatgtataa taactgataa
     4321 aaaatttgta gaagaattgt tggaatttac aactgaattg gtaagaagat atgctgtagc
     4381 tgcaaataaa gcaggagcaa aatatatttc aatagcagag ccaacgtcag taaccctaag
     4441 taaagataga tttgatgaat atatagtaaa aaatttaaat aaaatctaca atgaacttga
     4501 ttgttggaaa ggtatgcata tatgtggaga caccagagaa ttattagata atatgctaag
     4561 ttgtaatata gatgcagtaa gtttagacca aattttagac tatgaagaaa ttgctccaat
     4621 gattcctaga gatatagtgt tgattggtaa tttagaccca ataaaacttc ttggacgttc
     4681 taagccagat aagattagaa gagagacact aaaattgtta aagaagatga gaggatatga
     4741 taacttttta tgtgattttg gctgtaactg cttaaatacc actccagtag aaaatttaca
     4801 agcagctata aaagcaggaa gaattaatta taaagaatta gataggattg atattgatga
     4861 attataatag taataaaaat aaatatcttt taaaggatat aaatttagag gatattttta
     4921 tagataagaa tattgtgcta aagtttttgg ggtatggaaa tagaaaagct ccagatacaa
     4981 ttagtgaaat cgttgaaaga gaaataagaa atataaatga tattttagat atcaagatat
     5041 atataagtga agttgatatt aataaaacac ctaaagaatg taaaaaagca tttgctgtac
     5101 tatatacaat aggggataaa attgatatta agatgaatga ctatatggaa aattgcaata
     5161 tgatggcagg gatagcattg gataaaatag gtatagtatg tttagattat attaatgaga
     5221 aaataaaaat gtatttaaaa ggaaagtatg atagtcttaa aatatcgcat gaaatatatc
     5281 caggagataa agattttgaa gtagagagac aaaaggatat ttataactat gtaaaaaata
     5341 aatataatag tatagaaata gaaatcaatg attatcatca actatccccc ataaaaagtg
     5401 ttgcaatgct tattttaatg ggagatgaag aaaatttaga atcaagatgt agcaaatgtc
     5461 ctagaaaatg cttttaacta gttttcaaga ctaagatttg ttttgtgtat gaattgcaat
     5521 aatttttttg gtcattttat gtataaacta aaaaaattat tgcaaagtaa tattctattt
     5581 aatttgaaca gacttacaaa ctatagtagt ttctttaatt caataagtgt atttatttca
     5641 taagttggta tatgattagt ataatttata ctgttattgg aatttaacca acaagtatct
     5701 attcctgaat ttattccacc taagacatca gaggttaagc tattcccaat cattaaggca
     5761 gaagatttat catgacaatt aaactttttg agagcgtatt taaatatttc aggatttggt
     5821 tttgatattt tcaactcttc agatacaacc attccatcaa tatatccttt tatttttgaa
     5881 ttttcaagtc ttttcttttg aactgcagaa attccatttg ttattataac aatagtatac
     5941 ttttttttaa gatattgtaa tatatcgatt gcacctggaa ttaaaaatgt acattcacct
     6001 aaaaaattta agtacatttt gctaagagtt tcagaatcaa catccaaaga tattttattt
     6061 gcaaaaagtt caaatctaag taatttaagt tcattcaaag ttataatatt tttttcaagg
     6121 tcgagccaaa gcttatcact tatatttcta tatgactcta tataattctc aaaattaaat
     6181 tctaaatcaa actcagatag aagtttttta aaagcatgtg attcactttt cttaaaatca
     6241 aataaagtat catctgcatc aaaaaatata aatttatatg tcttcatggt ttttactcct
     6301 ttacaagatg actgcaaaaa ttactttact ttcactattt gagtcatttt gccatttatg
     6361 ctttgttcct aaaggaactt ttacactatc tcctttggac aaagtaaatt catcatcatc
     6421 cataaaaagt ttaacttctc cctccaaaac atatgcaatt tcatctccat tatgagtaat
     6481 cctatcaata caagaagaag tatttggagc taaatccata attgcaaatt ctaaattacc
     6541 actagagtct ggaattaaaa tctcatatat aacttctttg cttccaggta aaataacttt
     6601 ttttctatta tcatgtctta ctatcaagtt ttttttatct acttcagata caaaaaatgt
     6661 aaataaagga acgtttagag aagaggctat agattttaat gaatttacag atggattagc
     6721 aagacctcgt tctatttggc tcaataatga tggtgtaaca tcagctagtt ttgctaaatc
     6781 tcgaatactt aagttttgtt tttttcgaac ttcagctatt ttttccccta aatttaaatc
     6841 attcatataa atatcctctc tttttaattt aaaaaatatt aacttaaaat aatcaagtaa
     6901 aatgattatg tagtatgaaa ttattataac atatagaatt aggttttgaa aaaactatgt
     6961 tcaatataat taaataaatt aaaatataat tgacaaagtg gatacagaat acttaaaata
     7021 tatttaaata aaattaagca aaagatagtt aatttatgat aggaggggac attatgcaaa
     7081 taacgacttt tggagctata tttggtttat taatagcaat tattttaatt ataaaaaaat
     7141 ttcaagctgt atatagttta atgttaggag catttattgg aggattagtt ggaggagcaa
     7201 atataacaca aactgtagat tttatggcaa atggagctat gaatatatca ccatcaattc
     7261 ttagagcttt ggcatcaggt gtattagctg gaagtttaat aaaaacagga gcagtagata
     7321 aaatatcaga acagattgta aaaatatttg gtgaaaagag ggctttattt tcgattgcca
     7381 tttcaactat ggtacttgct ggagtaggcg taaatttgga tgtatctata ataactgtag
     7441 cacctatagg attatatata ggtagaaaat taaattattc taaattgtca atactattag
     7501 ctatgttggg agggggtaaa gcaggtaata taatatcacc aaatccaaat actatagcag
     7561 tagcagataa tttttctgtc aatctctcta gtgttatgat ggcaaatata attccagcaa
     7621 ttattggagt tgttattaca gttattttag caagtatatt aataaataaa ggaaataaag
     7681 tacaatcata tgaaatttta gagcaaagag aagatttacc aagtttattt aaatcattat
     7741 gtggtccaat tatagctata tttctattgt ttttaggaaa tgtatctcct atagttatag
     7801 acccaatgat agcccttcca ataggaggaa tcgtaactct aattgtcaca ggaaacttga
     7861 acaattcaag agaatattta gcttttggat tatctaagat gcaaggggta tgcatattat
     7921 tacttggaac agggactatc gcaggaatta tacagatgtc tgaattacaa cagagtacaa
     7981 taggtgcttt acaattttta aatatgccac aatttttatt ggcaccagtt tcgggtatac
     8041 ttatgtcatt agcaacagca tcatcaacag ctggtgctac tatagcatca tctacttttc
     8101 atgatgctat aataaatggt gggctatcac cgatttcagg tgcatcaata gtaaatgctg
     8161 ggtctagtgt attcgagcaa ttaccacatg gctcactatt tcatactagt tcaggtagta
     8221 taaacatgga tataggtgaa agatttaagt taattccata tgaagcattg ataggaatag
     8281 taatgactat aatttcaaca tctattcaat tagttttata ataaatataa atatttaatg
     8341 aaaaaagtag gattttgtta ttaaatattg aatttattat agtaaaaaag aagattggag
     8401 ttttaaagtg aataaaaaaa agattgttat tatagggatt atttattcat ttttagtagt
     8461 attttcactt acaaatatgt atgtaaatat ggagtataat ttaaatgtat ttgaatatat
     8521 taagaagtca cttccattta cagaagaaga aaaaaagtgg ttagaaaaac acaagaattt
     8581 gatttacagc tctgaccaaa gttcacctcc tttgagatat aagggtaagg aagatggaca
     8641 gtataaggga attattgttg atttaataaa ttctctgtca attcaaattg gtagagattt
     8701 ttattttaaa ccaaataatt ggtggaaaga atctttcgta aattcaatag atgatagtat
     8761 aaagtttttt gacttgattc catcaaaaga aagggcaaat aaatttatat ttactgaccc
     8821 aatatacact ttgagtgcaa atatattgaa agataaaaag tcccaagata taaatagtta
     8881 catggattta aaggggaaaa cagtagcaat tccagaaggt gattattcta taaatttttt
     8941 aaagcaaaaa attcaagata taaacatttt attgactcca gatataaaga ctggagtcaa
     9001 ccatttaatg tctggaaaag tagacgcagt tgttggtgat gaaccagtat tgcgatacta
     9061 tataaataat tatggacttt caaataaata cagtgtttta agtaatccta tatatactaa
     9121 aaaagcagtt cttgctgtac ctaaacaata tgaagagctg gttagtattt taaataaagg
     9181 catttttaaa cttcagaaaa atggagtgta taaagattta aagaaaaaat ggtattctac
     9241 gtataatgaa gttgatgata tattgtatga gcgaggaatt gtgcctagta tttatctttt
     9301 tataggtata attttaatat caatttatgt tttttatagc tatacatatt tgttaaagat
     9361 agaaatcaaa aggaaaacag aacaggtaat agaaaataaa aaaactttag aggcaacttt
     9421 taatagtatt acagatataa taatgcttgt agatgaaaat aacaatatag tagaatcgaa
     9481 caaagtacta tatgacttta tgggtgaaat gagttataag atagcagatt tgattagcat
     9541 gataaaaggt gttattaaaa ataccttctc tgaaaatact aataaaacta gtgaaataga
     9601 aatatacaat aaaattttaa agataaacac ttttcctgta gagtataaaa aaaataatac
     9661 tgagtatata gtagttctta ttaaagatat aactaatgaa aaaatagtag aggcgaaatt
     9721 acttagagaa aacaaaatga tatctatagg acaattagct tctggggtag cacatgagat
     9781 aagaaatcct ttgggtataa taagaaataa ttgctatcta ttaaaagata acgttacaat
     9841 ggaagaagtg aatgattgtg ttaaatcaat agaaagtaat gtagataggg caagtaatat
     9901 aataaccaac ttgttaaatt tcgcaagaat atcagatgat aatttagaac atataaatat
     9961 aagaaatttt attgaaaata tagtgaaact tcaatataaa atgttacagt taaagaatgt
    10021 tgaaataaaa attgactgtg aacataattt agtatgctat ataaatggag aatcgttaaa
    10081 acatgtattt ataaatttaa tttctaattc aatagatgca atacatcaag atggaaaaat
    10141 tattatatac tgttatgaaa aaaatcattg tttatttata gattttaaag ataatggtga
    10201 aggaataaaa gaagatgccc taaaggacat atttaatccc ttctatacaa caaaaccaat
    10261 tggtgaggga actggtttag gtttatatat tacttataat gagattaaaa agaataatgg
    10321 agatataagt gttgaaagta aattgggggt tggaacttgt tttcatataa aaattccatt
    10381 aaataaagag gtgactatat aatgaaaata ctattagttg atgatgagct agaatatgga
    10441 gtggtaatga aaaaaattct acaaaagaag ggctatcttg tagatatagc actaagtggt
    10501 gaagaagcta taaatataat taaaaaagat aaaaattatg atttggtttt atctgatgtt
    10561 atgatgaaaa atatggatgg agtacagcta ttagatagaa ttaaatctat taatagagat
    10621 atagaggtta tattagttac aggatatgga agtattgaaa atgcagttga tgctatgaaa
    10681 agaggagctt tatcttattt tataaaaagt aatcccattg aaaatttatt agaagaagtt
    10741 gaaaaggtaa aggcaagtaa aatatctgtt tctgtacaaa aaaataattt tgaatttact
    10801 ttggaatcta aaaatagaga ttttaataat atcataaaaa tagcaaaaaa agctgcttgt
    10861 aaagatgtta atatattaat cttgggggaa tctggtgtag gaaaagatat attagctaga
    10921 tatatacata gtataagtcc aagaaagaat gagatatttg tacctgtaaa ttgttgttct
    10981 ttttcagaaa atctattgga gtctgagttg tttggtcatg aaaaaggttc ttttacagga
    11041 gcagtagata gtagaaaagg tagatttgaa ctttcgaata aaggaactct atttttggat
    11101 gaaataggag atatacctct aaatgttcag gtaaaacttt tgaggacact tgaggacaaa
    11161 tctatagaga ggataggaag taataaatct attaaagttg actttaggtt aatttgtgct
    11221 atgaataaag aaccaaaagt ggaaatatca aatggaaata taagagaaga ttttttttat
    11281 agaataagta caataacaat aactatacca ccactaagaa agagaagaga agacttaact
    11341 actcttatag aatttttctt aaataagtac caaattgaac atgataaaaa gatacactcg
    11401 attgataagg aagtaaagga ttttttatta aattacaatt atccaggaaa tataagagaa
    11461 ttaaaaaata taataaatag acttgtagtt ttaagtgaag aaggcaattt atcaaaagac
    11521 aatttaaatt taatatcaaa taatgtatac atagatgata aaataagtat tagaccttta
    11581 agagaaataa gaaaagagtt tgaatgtgag tatattgaaa aagttttatc tttgtgtgga
    11641 aataatattt caaatacagc taaaaaattg gagattagta gaagacagtt aaccaataaa
    11701 atatcagagt ataatattaa gtgagaaaaa aacttctcat taagtgagaa aaaaatttct
    11761 caaactgtat taaaagataa agagaaaaaa tctgtttatc tttttttatt ataattttaa
    11821 aaattaatat ttacaataat tattggtaaa ttcaatactg gtgtaaatta aaaatgaaaa
    11881 tttctatgac attaatacat agaatatata ttttaaataa ttggcataga aaatgcttta
    11941 tatattaata aacgtaataa atatttttaa ttgcgattaa atatattaag ggggattttc
    12001 atatgaagct agagctaggg aatatattta taaaaaatgt tgaatttgga gaaaagacag
    12061 aagtaaaaga tggagtacta tatattagta gcgaagaaat agaaaagata gtattagaag
    12121 atgaaaggat tgtaagtgta aatgttgaac ttgcaagacc tggcgaatca ataagaatag
    12181 ctccagttaa agatgtaata gagcctagag ttaaaattgg agatgaaagc aaaatatttc
    12241 ctggaattat aaataaagtg aagactgtag gaagtggaag aacccatgta cttttaggag
    12301 cttgtgttgt aacttgtgga aatatagttg gatttcaaga aggcgtaatt gatatgagtg
    12361 gtcctactgc taaatataca cctttttcaa aaacaaataa tatatgtatt gttataaaag
    12421 caaaggatgg tatagatact catgattatg aagaagcagc tagaattgca ggattaaaaa
    12481 taggtgcata tgtaggtgag gcaggaagag aagttgagcc agatgaagtc attgtatatg
    12541 agacaaagcc attattaaaa caagtaaaag aatatccaga cctgccaaca gtagcatatg
    12601 ttcacatgtt acaatcacaa ggacttttac atgacactta ttactatggg gtggatgcta
    12661 aacagatagt gcctacattt atgtatccaa ctgaaataat ggatggagct ataatatctg
    12721 gaaactgtgt agcaccatgt gataaggtta caacattcca tcatttaaat aatccagtta
    12781 tacatgactt atataaaaga catggaaaag atttaaactt tataggagtt attttgacta
    12841 atgagaacgt attcttagtc gataaagaga gaagctctga tatggtagct aaattaatag
    12901 aatttttagg agttgatgga gttctagtga cagaagaagg ttatgggaat ccagatactg
    12961 acctaatgat gaattgtaga aaatgtagtg aagttggagc aaatgtagtt ttgataacgg
    13021 atgaatttcc tggaaaagat ggcaagagtc aatctatagc agacgctact aaggaagcag
    13081 atgcagttgt ttcttgtggt caaggaaatc tagtggttca tttcccagca atggaaaaaa
    13141 ttataggaac tttagattat gttgaaatga tgataggtgg gtataaagga tgcttaaatg
    13201 aagacggaag catggatgca gagctacaaa ttataatagc atcaactata gcaaatggct
    13261 ataatcattt gacagcaaga tattattaaa taagtatttg aatataaagt tctgttgatg
    13321 taatcgttgg tctaattgat gtaataacta taaagtgatt tggaggatta agagatgagt
    13381 aaaatcagag tggttcatta tataaacaat ttctttgctg gtataggtgg agaggaaaaa
    13441 gcagatattc cgccagaaaa aagggctggt gtagtaggac ctggaatagc atttcagagt
    13501 caatttaatg atgatgcaga gataatctca actgtaattt gtggagatac gtattttggt
    13561 gaaaatattg agagtgcaac tcaagaactt ttaaaaatga taaaagaaga aagccccgat
    13621 ttatttattg caggcccagc atttaatgca ggtagatatg gagtagcttg tgggactatt
    13681 tgtaaggcag ttgaagaaga gttaaatatt cctgtagtta caggaatgta taaagaaaat
    13741 ccaggtgtag atatgtttaa attagatttg catatagtgt ctacaggaaa ttcagcagca
    13801 agtcttagaa aggtagttcc aataatgacg aaactaggtc ttaaattggt tagaggtgag
    13861 gaaataggac ctcctgaaga ggaaggatat ataatgagag gaataagaaa aaatttcttc
    13921 catgaactta gagggtctga aagagcaatt gatatgttgg ttaaaaagat gaaaggggaa
    13981 agctttgaaa ctgagtatcc aatgcctgaa tttgatagag ttacacctat gaaacctgtt
    14041 aaagatttat ctaagataaa acttgcccta gtaacttctg gagggatagt tccaattgat
    14101 aatccagaca aaattgaatc ttcaagtgct actaaatatg gaatttacga tttaacagat
    14161 atggattcta tgtcaaataa agaatttaca actatacatg gggggtatga cagagcatat
    14221 gtccttgaaa atccaaattt agttgtacca ttagatgtag ttagagagct tgaaaaagaa
    14281 ggtgtaattg gagaattagt agattatttt ataactacta caggaacagg aacgagtgtt
    14341 ggcaattcta agagatttgg agaagagttt tctaagaaat tgttagaaga tgatgtagat
    14401 gctgtaattt taacttctac atgaggtact tgtactcgtt gtggagcaac gatggtaaaa
    14461 gagattgaga gagctggtat agcagttgtt catatatgta cagtagtacc aatatcactt
    14521 acaataggag ctaatagaat tgttccagca gtagggatac catatccact tggaaatcct
    14581 atgttaggag aagaggaaag taaaaaaatt agaaagaata tagttttaaa agctttaaat
    14641 gcactaacag aggatataga agaacaaaca gtatttgaat aataaaaata taatatagaa
    14701 ataaaaaaag atatcataaa atagtttttg aaaattattt tatgatatct ttttgctatg
    14761 gatggaatga agaaagcaat taagtaataa aaatatttat agtaaaacag ccaattaaag
    14821 tataaagaat gaaaaaaata aatagaagat tatacataaa caaaaaaaga gattttaaaa
    14881 tctcttttta ttatatatta attatttttt aaatttatat tttttgcatc agattttcta
    14941 atagctaagt caaaccctct aaatctaatc actatagggt cattaagagg agctatcttt
    15001 ttcacttcaa cttcagtgcc atctattagt cctaaggcta gaagtctctt cataagtttt
    15061 tcatttccag ctatattgtc tataattccc ttttgtccaa gttttaagtt gtaaacagtc
    15121 attattaaca cctcctaatg aaaatcatta tcaaacttta tatttataat atataacaaa
    15181 aagagtaaat tgtcaatata taacaaaaaa taaacatact aagttagtat gtttataaaa
    15241 aatagtatac atgggatact aatagcatct agcaaaaaac cacttactaa tggtacaacc
    15301 aaaaaggctt ttggagaatc accatatttt tgtgtcaaag aactcatatt tgccaaagcg
    15361 ttcggagtag ctcctattcc atgtccaatc aaaccactta ttatgatggc agaatcaaaa
    15421 tcttttccaa gtactctaaa gcaaataaat attgaaaata ggattataaa gattacttga
    15481 aaagttacta ttaagagaat aggaggtaat aatgtagata attcaaataa atctatattc
    15541 ataagtgata aagttaaaaa aattcccaat gaaagttctt ttaaaaaatc tataagttta
    15601 aaatttaatt ctaatatatt tatcttatca tttaaatttc taaaaataac agctgaaaac
    15661 atacaagtta caacagaggg taatagtata ttaaacgtta tatagaagca tctacttata
    15721 atttctcctg tatttataca aataaatatg agaagaacct gttccaaaaa tacagatggt
    15781 gttattgttt cggagaagtt attatttttt atcctattct tatttctaaa aaatttactt
    15841 ccaaatctct ttagatttct attatatcta ggaatcgaca agcctatatt tgatgatggt
    15901 tttaatttat atttatttat tagaaattta gctacaggac atcccaatat accacccata
    15961 ataagtccta ttgttgcagc agataatcca accccaactg ctccttctat accgagattt
    16021 tctattgtaa gtccaaatgc agcagaataa ccatgaccac cttccataga tacatttcca
    16081 cacataagcc ctaaaagagg ttcaagtttt ataatctttg atattgtaat agctaatata
    16141 ttttgacaaa atgccaatac tccacacaaa atccaatatc taaataatac actaccacct
    16201 ttctttataa gatttatgct tatttcaagt cctatagttg taaaaaagaa ggttataaaa
    16261 tttggcatta aatttccact tatagatata tctgctatat taaaatattt aaaaaataaa
    16321 tttagaaaac aaaataatag acctcctatt actggtgcag gtatacagaa attatttaaa
    16381 aagttaactt tattcttcaa taaattacca attaaaaaga agaatataga tagtgtcaag
    16441 gtttgcacta tatcaaaact taaggtaatc aaaataagtc ctcctttgta attcaaagtt
    16501 ttaattatat aacttaaatt cttatgtatt gaaattttat tatcaaatgt atattaataa
    16561 atgtatatta aaaagaatca gtatatataa ttagatagtc ataaattaaa ttaaaatatt
    16621 ttaatgagat gtgacttttt tagaatgaaa aaccgtagta tattgatata atagaaataa
    16681 gacttaaaaa tctgttgata gtattattta taagtacagt aggaggataa aatgtttgaa
    16741 aatttaccta agatagattt acattgccac ttagatggaa gtgttagagt agaaaccatg
    16801 cttgatatag ctattaaaga gaaaatagat ttaccttcaa ataatatgga tgaaataaaa
    16861 aaattagcaa aggtttcttt taattgtact tcactagatg aatatttaga aaaatttgat
    16921 ttacccttaa aagtaatgca atctaaggaa aatttaaaaa gaattacttt tgaacttttg
    16981 gaagatgcaa gtagagaaaa tgtaaaatat attgaaatta gatttgcacc attacttcac
    17041 acccaaaaag gtatgagtgt taaaaatata attgagggta taattgaagg aattagagaa
    17101 gctgaaagta tttatgatat aaaaggcaat ttaatattag gttgtatgag aacaatgacc
    17161 tcaaaagagg cattattagt aattgaagaa gggaaatcat ttgtaaataa aggtgttgta
    17221 gcagtagact tgtgtggtcc agagaaagag ggattttgta aggaatataa agatgttttt
    17281 aaattagcaa gagaatatgg atataaagtt acaatacatg caggagaagc tgcaagtgga
    17341 gaaaatgtat tggatgctat aaatatttta aaggcggata ggattggtca tggggtaaaa
    17401 attaaagacc ataaaaaggc gtataattta gttaaagata aaaaaatatt gcttgaatta
    17461 tgtccaacta gtaatgtaca aactaagaca gtagattctt atgaggtaca tccattttat
    17521 actttttata aagataattt acatgtaagt ataaatactg ataatagaac tgtatcagat
    17581 ataaatttaa gttcagaact aaatgttata tttgatactt ttaaactagg attagaagac
    17641 tataaaatta tttatagaaa tgcagtagaa gctagttttg cagataaaga aactaaagag
    17701 tatttgaata gtttaattta atagataaaa atatttaaac tttaaattaa gctattaaat
    17761 tgacaaaaat acattgtatt aggagaaaaa atgtgaaaga ttatgaagat gttgtttata
    17821 ttaaactagt caaaaaagaa cttgaagaat ggaagaaaaa tatattaaag aaaccttcta
    17881 tatttgacaa agcatcaaag ggtatacaga ataaatttaa tggagtattg ccagataaat
    17941 atcatgaaat aattacttct tctataaaag taatgacaaa aaccgtactg tttggatata
    18001 aatatgctac aaagccacca cttagaaata tgtcattaga agaaagagaa aaattagttt
    18061 tagaaaaagc taaaacttac aagaccacag caatgattga aggggcagga acaggggcag
    18121 gaggaatcct cttaggttta tctgattttc ctttgctttt aggtattaaa gtgaaatttt
    18181 tatatgatgt agcttcaata tatggatatg atgttaaaga ttatagagaa aggatttata
    18241 tattaaacat attacagttg gctttttcta gtcaacagca tataaacaaa acttttaaat
    18301 tgatgaataa ctgggatgaa tatgtaaata gtctaccaga agacatcaat gattttgatt
    18361 ggagaacatt tcaacaagag tatagagatt atttagattt agctaagttt ttacaactaa
    18421 tgcctggaat aggagcattt gtaggatttt atgtgaatgg aaaattaatt gataaattat
    18481 gtgaaactgc tgtgtctgct tatagattga ggattcgaga gcttaagtta taagagaaat
    18541 tataacataa aaaattagtt atacaatata tctatatgta tctaattttg cattgataaa
    18601 aacaattatg gataaaaggt aaagcatgat agaatctaat gtagaggttt gtattttaat
    18661 ggaaaaattt agtttttatt gcttaagata caaattgaat tacgaagaga agtaattggg
    18721 aggaatattt tcatgacaaa tttgccaaaa caatttgata gcttcaatga agctagacaa
    18781 aagggattta taaatgcaaa ggaacttaag gaaagtggga aaaagatggt tggagttttc
    18841 tgtacattta ctccagttga aattccaatg gcagctggag caactgtagt aggtgtatgc
    18901 ggtgttagtg aagagccaat acctgatgca gaaagggtgc ttccaagaaa tctttgtcct
    18961 ttaattaaat caagctatgg acatgcaata acagacacct gtccttattt ctatttttct
    19021 gatttgttga taggagaaac cacttgtgat ggtaagaaaa aaatgtatga agaacttgca
    19081 aaagttaagc caacatatgt aatgcatttg ccaaatactt caaaaggaga atttgcctat
    19141 aaactttgga aagatgaaat gataagatta aaagaagagg ttgagaaatc tttaggtgta
    19201 actataactg aagaagatat aagaactgct ataaaagata aaaatgaaga aagagaactt
    19261 ttaaaagagt tttatgcact tggtaaatta gaaccatctg cacttacagg ccttgagtta
    19321 cataatgttt tatatcaagc aggttttaaa tttgaccgtg cagaacttaa acattcttta
    19381 agaaaagtta tagatgatat gaaagaaaga tatgaaaaag gtgaatgtcc tgttcaaaaa
    19441 gataagccaa gaatattgat aacaggttct cctattggtg gaataagcga aaaaatagtt
    19501 aaaactttag aagatgcagg ggcatcagtc gttgcatatg aactttgtgg agctattaga
    19561 tcaaacgatt tattggttga tgaagaaata gaagatgtat atgatgcttt aacacaaaaa
    19621 tatataaata taggttgttc ttgtatgatg aataatgaca atcgtattga attattagat
    19681 agaataatag atgaatataa tgtagatgct gtaatagatg tagttttaca agcatgtcat
    19741 acatttaata tagaaagtta tagaataaga gaatttgtta caaaagaaaa aaataagcca
    19801 tttatgtctc ttgaaactga ttattcaaaa tcagatactg aacaattaag aactagattt
    19861 gaagcgtttg tagagatgtt gtaggaataa ataaaagtag gaggaactat gtttagtata
    19921 ggagttgact cagggtcagt ggcaacaaag ggtgtgctat ttgatggtga aaaaattatt
    19981 aagaaaataa ttataccaac tggatggagc cctaaaaata catctaaaca ggtctatgag
    20041 ttgctaagta gtgaaattga caaaaaagat ataaaaaagg tggtaggtac aggttatgga
    20101 aggggagtaa tggattttgc tgataagaaa gtaacagaaa taacttgtca tactagaggt
    20161 atttactttt taaacaagaa cataaggact attttagatg taggtggtca agatagtaaa
    20221 gttataaatt tagatagaga tggaaatgta tttaacttta taatgaatga taagtgtgct
    20281 gcaggaactg gtcgattttt agagattact tctaaccttt taggaagcga tattgaaagt
    20341 atagatacat tagctaaagg atatgaacca gttaatatat ctagtatgtg tacagttttt
    20401 gctgagtctg aaattgtaag tcttctagct caaaacattt ctactggaga ggtagcagca
    20461 gggatactta aatctattgc aaataaatcc acctcaatgt tagcaagagg tgaggtaata
    20521 gatgaagttg catttactgg agggcttgca aaaagtaaag aactagtaaa aatgatagaa
    20581 gaaatactgg gtaaaaaaat ttttattgca gaagatactc aaataatagg agctttagga
    20641 gctgctgtaa taggatttag ataaaaacat ataaaaagaa aatgaaaagc tgaatttatc
    20701 ataaataagt tcagtttttt attttatttt gtttatttat aaaggaaaac tttggtataa
    20761 ttgatttaaa tgattgtata tttgtaaata cttgatattt agtcatatat aacattagaa
    20821 ttttaaaaat tagctcaaaa taagggggag aaagcgtgaa aaaatttact tcaaaaaaag
    20881 tagcaaggtt tgttgcactg tttttaatat ctattttagt aatgtcaaca attcctgtgt
    20941 cagctgacaa tagcaataca ttgaatcaga taaaaaaagc agaatacagt gatacttata
    21001 aacaatatct aaatgatgct aaaaatggac gtacagaaaa atataatgga attataccaa
    21061 atccatatca gttagaaggt actcaaattc aaagtaaagg aagaacagct ccaacatctt
    21121 atgaccctag aaaattaggt cttatgacat cagtaaaaaa tcaagaggat ttaggaatat
    21181 gctgggattt tgctgcaatg gcaaccttgg aaagtttctt gaagttaaat aattatggag
    21241 actacgattt atcagaagaa cacttaagat ggtgggcttc agatggtgaa tatggatggt
    21301 ctgtaaatga tatggatggt gctttaaatt atgaagctat gggatattta acatcttggt
    21361 caggtcctaa attagaaaaa gatatcccat acaatggtag agtatctaaa gcacaaggag
    21421 caaaaaaacc aactaatatg aatacagcac ctactgtttt taatgtgaca gatgctgtgt
    21481 gtgtaagtaa tgacataaat tctacaaaaa atgctatatt acagtatgga gcagtaacat
    21541 ctggttatta tgaagatatt aaatatcaaa gtgatgacca aaattcatat tattgtccaa
    21601 caaaaagttt taatacaaat catgctattt caattgtggg ttgggatgat aactattcta
    21661 aagataattt taatgcaaat attagaccat caaaaaatgg tgcttggtta ataaaaaata
    21721 gctggggaga ttataattct gagggtggat atttctggat atcttatgaa gacaagacac
    21781 ttatgtctga tatagataat ttttcaatca aaggtggtaa aaaaccaaat gatgatgaga
    21841 agatgtatca acatgactat gcaagtattg tacctttaat ttcaaaaaag ataacagctg
    21901 caaatgtatt tgatttcaat agaggcgatg aaactctaaa atctgtaatg ttcttaactg
    21961 aaagtatagg agctaagtat gaagtatctt atgctccagt agtaaatggt atacctcaag
    22021 aaaacaatat gaaaaaatta aaagaaggta ctgcccaata ttcaggatac ataacagttc
    22081 caattgattc ttttaatata ccagaaggaa agggtgcaat tgtagtcagt atagagggga
    22141 aaaatggaga gtcaactata ggttctgagt caaatgtacc aggatatgat acatttaaag
    22201 caaaagctaa tttaggtgaa agttatatca tagataatac tggaaaattc tttgatataa
    22261 atagagatag taacttctat ccgtgtaatt ttactataaa agctgttaca gaaaagtctt
    22321 ctggtgagag tatcccgaat gagtctttaa ttggtagtga tagatatgaa actgctatca
    22381 aagttagtca aaatggtttc aattcttcag aaaatgtagt tttagtaaat ggcagttcaa
    22441 tagtagatgc attggcagca actcctttta catctgctat aaattcacca atattattga
    22501 cacaaaaaga agctttaaat tcaaaaacta aagctgagat acagagatta ggtgctaaga
    22561 aagtatacct aataggcgga gaaaattcta taagtaaaga aattgaacaa caattaaaaa
    22621 gcttaaatat atcgatagag agaatatcag gaagtgatag atacaagaca agtttattat
    22681 tagctcaaaa gttaaatggt ataaaaaatg tatctcaaat agcagttgta aatggagtga
    22741 aaggattagc tgatgcaatt agtgtaggtg ctgctgcagc agaaaataac ataccaatca
    22801 tacttgcaaa tgaaaagagc gaattgcaag gagctgatga atttctaaat tcattaaata
    22861 tagagaaatc ttatattatt ggaggaacag catccctatc aaacaatcta gaaagtaagt
    22921 taaaaaatcc aactagatta tctggtagta gtagagacga aactaattca aagataatag
    22981 ataattttta taaaaaagat actttaaaaa atgcatttgt agttaaaaat ggaatcaaga
    23041 atcaaaatga tttaatagat ggattggcag ttggtccttt aggagcaaaa actgggtcac
    23101 cagtaatttt agttggtgat aaattagctg atagccaaaa agaagttctt aaaaataaga
    23161 ctttagaaaa agtaactcaa gttggtggag gagcaaataa aaatgcattt aaagagttag
    23221 taaaattaaa aagtgctaaa tagagtactt aagtattaac atatattagt gtgtat
//
LOCUS       DAELXX010000062        23103 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.062, whole genome shotgun sequence.
ACCESSION   DAELXX010000062 DAELXX010000000
VERSION     DAELXX010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23103
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.062"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(118..1074)
                     /locus_tag="KPH29_002235"
     CDS             complement(118..1074)
                     /locus_tag="KPH29_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBG1467824.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVDIP
                     GRGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            1442..2230
                     /locus_tag="KPH29_002236"
     CDS             1442..2230
                     /locus_tag="KPH29_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG1467825.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVDDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(2353..2823)
                     /locus_tag="KPH29_002237"
     CDS             complement(2353..2823)
                     /locus_tag="KPH29_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HBG1467826.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            2962..3606
                     /gene="uppS"
                     /locus_tag="KPH29_002238"
     CDS             2962..3606
                     /gene="uppS"
                     /locus_tag="KPH29_002238"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HBG1467827.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            3649..4554
                     /locus_tag="KPH29_002239"
     CDS             3649..4554
                     /locus_tag="KPH29_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG1467828.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHLNRKKRKLNKKKLAVLICFTVLFLFIAFKATQGVVALVKSMDKSNKTSQQQKVNSE
                     QFDLGNEEENKKKKYTVFIDPGHGGNDKGTESKTSNRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQTVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSEEQKIIN
                     EKYQDQLAEGIVQGVLSYLDSKGNK"
     gene            4869..6416
                     /locus_tag="KPH29_002240"
     CDS             4869..6416
                     /locus_tag="KPH29_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG1467829.1"
                     /translation="MNKNSMTKIYSLNKIIFIIIGILGIVLLTRHIEVDIKVLILILL
                     TLFSINVFISYLKIKLYEDKINLGLINNSDKFINITKDENFLKHVQNYIISNEKENCV
                     MACFDLCRLKLINDIYGYELGDEVLNNILSNLKNYFGKEAIYGKLKSDVFVLIIKLEN
                     REQKIPYLVNLIKNKIMILSQIDSFKMDINIEASIGIYKFNNNEIDIKKAIDNADMAR
                     LKSKGLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVDSSTGNIIG
                     SEALIRWLHPSLGMVGPNKFIPIAEKNGLINNIGRWLIQEVFITINKWISEGINVLPV
                     SINLSRVELYKNDLVDFFKLMFNTYNIPKELIELEITETTALRDVKFISERLYEIKSL
                     GMNISLDDFGVGNSNFINLKGIPLDIIKIDRSLILDIVTNTKTKFMVKAIINLSHDLN
                     VTVICEGVEDMHQVKVLSELGCNFIQGYVFFKPLDEMNYKKLLTDGSYINLKDTLLDK
                     CMTVEEE"
     gene            6419..6817
                     /locus_tag="KPH29_002241"
     CDS             6419..6817
                     /locus_tag="KPH29_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBG1467830.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            6857..8590
                     /locus_tag="KPH29_002242"
     CDS             6857..8590
                     /locus_tag="KPH29_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBG1467831.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGVGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEY
                     RLIYSNDIDNENTNKKILDEIIGLYKTKLENRRECKSGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEYLVGILNSSVYDKYFKI
                     TAKKMSKNIYDYYPNKVMKIRIFRDNNYEEIENLSKQIISILLNKSIDKGKVEKLQIK
                     MDNLIMDSLGI"
     gene            8822..10033
                     /locus_tag="KPH29_002243"
     CDS             8822..10033
                     /locus_tag="KPH29_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBG1467832.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIIQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            10225..10485
                     /locus_tag="KPH29_002244"
     CDS             10225..10485
                     /locus_tag="KPH29_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBG1467833.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
     gene            10838..12550
                     /gene="ptsP"
                     /locus_tag="KPH29_002245"
     CDS             10838..12550
                     /gene="ptsP"
                     /locus_tag="KPH29_002245"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBG1467834.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASIHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVLNMGTAEEIKSYIEK
                     TFNI"
     gene            complement(13342..14007)
                     /locus_tag="KPH29_002246"
     CDS             complement(13342..14007)
                     /locus_tag="KPH29_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467835.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDNLIPAKITSEQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAG
                     NLN"
     gene            14351..15781
                     /locus_tag="KPH29_002247"
     CDS             14351..15781
                     /locus_tag="KPH29_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBG1467836.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKALKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEDQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEEICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
     gene            16149..16727
                     /locus_tag="KPH29_002248"
     CDS             16149..16727
                     /locus_tag="KPH29_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBG1467837.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENINI
                     DLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKFVYKKTAIFISTVVLLITILSL
                     SLSIFVDYFTYSAFAMFWIFVMLVLGKLKAKV"
     gene            16757..16903
                     /locus_tag="KPH29_002249"
     CDS             16757..16903
                     /locus_tag="KPH29_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBG1467838.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKTN"
     gene            17208..18587
                     /gene="scfB"
                     /locus_tag="KPH29_002250"
     CDS             17208..18587
                     /gene="scfB"
                     /locus_tag="KPH29_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HBG1467839.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            18616..19113
                     /locus_tag="KPH29_002251"
     CDS             18616..19113
                     /locus_tag="KPH29_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBG1467840.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            19411..21075
                     /locus_tag="KPH29_002252"
     CDS             19411..21075
                     /locus_tag="KPH29_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBG1467841.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEPIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            21088..>23103
                     /gene="recJ"
                     /locus_tag="KPH29_002253"
     CDS             21088..>23103
                     /gene="recJ"
                     /locus_tag="KPH29_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBG1467842.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDISLIDLDFNINYNIIEDVSKNTDKLELIFSPNIDKIDLKR"
BASE COUNT         9375 a         2219 c         3885 g         7624 t
ORIGIN      
        1 atatattttt taaaagtaat ttatatttta aaataaaaac acaccccata aatacaaagc
       61 aatttgcatt taagaggtat gtatttatca tcaaattatt ggtttgtttt ggaatttcta
      121 atcttcaacc ataatatctt taaatatgaa atcatgcatt gcatctagtt catattcctc
      181 aaaaggtatt acatagcctg cattacctat ttttctacca ttttcagtag aaagaggaaa
      241 ctccatagat ttcaaattta aatttcctat acttaaaagt tcttttccta aagatattat
      301 ttcattagga ttcatattag tttttacata cggaagtatt gtgttaacta gattaggata
      361 tttagtgact ggtaaatcct taacaccatt tattaaactc tgtataacac ttctttgtct
      421 tctatctctt tcatctgtac tatcattttt tcttatacga gaaaatgcaa gtgtctgata
      481 tccatttaat ttttgctcac cagaatttct tatatattta agagaacctt tattgtcatt
      541 tttattccat ttataagttt caggaataaa tttatttaat tctcttattt cactcttatc
      601 aacagtaaca gtaactccac ctaaagcatc aactatatcc ataaaagaga aaaagtcaac
      661 tacggcgtag ttttgtatat caagttggaa gttttcttct atagtttcta ttaataagtt
      721 agcttgtcca tatacgtagg catgtgttaa tttttgttct ccacgtcctg gtatatcaac
      781 ataagtatct ctatttaatg aagttagctt aagactttta tttttattat caatagtaag
      841 tatcatcatg gcatcagaac gagaaacttc ttctcctggt cttgcatctg tacctaacaa
      901 taatatgttt gtgatatttt cttcgttttt atagtcatta ctactaagcg cctctgtatt
      961 tatactatca tcttgtatag tttttaattt agtaaaaaca tatccatata tactaacagg
     1021 aaatgctata actaaaaggg ctagaaatat aacgaacttt ttgagtcctg acaataatat
     1081 cccctacttt ctaaattttt taattacaca tagttaatta tattaaaaat ttgttacata
     1141 agcaataaaa aacacaataa attagcataa gtttgtcata atataattat ttttatagta
     1201 ttttatagaa gaaagtgtgt agttattatt gattttattg gaatataatt gtatgaagac
     1261 ttaaattttt ataaactgat gtgtatattg aaaaaaatat tttcctgaat gtagttatag
     1321 catcttatga aaggtgtaaa ttgtaattta aaatttttag tgtataataa gaattgaaaa
     1381 ttcaaaccga taaaaattaa taaaaaataa ttataaatta ataatttggg ggaggaaatg
     1441 tatgataaaa ttaatagcaa cagatttaga tggaactttg ttagatgaaa agagcgaaat
     1501 aaatccagag ttttataaag tatttaaaaa attaagagag agagggataa tgttttctgc
     1561 tgctagtggc aggcagtatc aaaatcttat aaaaaaattt gaagatataa aagatgatat
     1621 gatgtttata tcagaaaatg gaacactagt agtatataaa ggaaaagaaa tattatcaaa
     1681 tcctttaaat aaagaattag taaatgaaat catagagaca acaagaagta taaaagggaa
     1741 aaaaattgta atgagtggga aaaaatacgc ttatatagaa agcaaagatg aagcgtttat
     1801 acaagaggtt agtacttatt atgcaaagtt taaaattgta gacgatttga cgaaagtaga
     1861 aggcgatata ttgaagatag ctgtatttga cttcaaaggt gcagaacata ataataatat
     1921 ctactttgaa aagttttctg atagagctca agtatgtatt tctggagtgg agtggcttga
     1981 tttaactgca aaaggtgcaa ataaagggag tgccataaaa aaagtacaaa aaatgttaga
     2041 tataaaatat gaagaaacta tggtatttgg tgaccagtta aatgatgtgg aaatgatgaa
     2101 aagtgcctat catagttatg ccatggaaaa tgcaaatgaa catttgaaac aaattgctag
     2161 atttagagca aaaagaaata ctgaaaatgg cgttgtagat aagataaaag aggtcataaa
     2221 aattggatag tcatatgtat agatgaaaaa gattattttg aagaaatcaa aaaaatatca
     2281 atgtaatttt gataaataag tttatttatg cgaaaattac attgatattt aataaacatc
     2341 aataatagat atttattttt ttatgatatt cagtattttg ttttctattt gagttgcatt
     2401 ttcagcagaa attaaataaa catatctacc tgctttctca attatagtat tatttgctat
     2461 ttcagaattg attttttctc catgtccttc tccaaaaggt gcttgtatca tatctttttt
     2521 ataattttct agagttttat atattttatc tacctcgtca gtttttataa cgataatatc
     2581 ttctagagat aatttttcag aagaattaat tacaaaacct tgaattatgt cttcctctac
     2641 atcattaaaa tagttaaagt cctttgcatc aaagattttt gattctttta taagtaaatc
     2701 agaagatttt aatgcttttt ttatttttga tgtagaaacg tttttgtaat caccacttga
     2761 agaacaacta accattccta atgataagat tatcgaaaaa aacaatatta aaattttttt
     2821 catgataaac ctccaagttt tttaaactct gtcttaaatt agtttactac ataattatat
     2881 aaaaataaat gtatttaatg ttacagtttt gattaataat gtaatatttt gataataata
     2941 cctattgaga ggtgattaga aatgagaata ccaaaacatg taggaattat accggatgga
     3001 aatagaaggt gggcaagtca aaatggtatg acaaaagata aaggatatga aagtggaata
     3061 gaccctggat tagagtctta tagattatgt agagatttag gctgtgaaga ggtgactttt
     3121 tatggattta caactgataa tacaagacgt ccatcagtgc agacaaaagc ttttacaaaa
     3181 gcatgtattg atgctgcaaa tatattaata gatgaaggtg ctgctttatt agttgtagga
     3241 aatacaaaat ctccaatgtt tccagaagaa ttattaccat atactaatag aattaaagcc
     3301 aaagaaggtc aaataaaagc aaatcttctg gtaaattatg ggtggtattg ggacttaaat
     3361 aatatatcag gagtagaaaa tgtaaataaa aataatatac aggattacat acagtcattt
     3421 gatgtatcca gactagattt aataatcaga tggggtggaa gaaggagatt aagtggtttc
     3481 cttcctgtac aatcaattta tgcagatttt tatgttatag ataattattg gccagatttt
     3541 aagagtgacg atataaagga cgcatttagt tggtatgata cacaagatgt cactttaggt
     3601 ggataattaa aaatattctg atttaaggtt gtagagtgtt ttaaattatt gaaaaaaaaa
     3661 cttcttgatg gtaaaataac actaataata tgtaaaagtg taaaaattta taccaagaag
     3721 ggtgaagaaa tgtcaaaagg caataataac aataattcaa gaaacaaatc taaaaagacc
     3781 agtcatctga atagaaaaaa gcgaaaatta aataagaaaa agttagcagt gttgatttgc
     3841 tttacagttt tatttttatt tattgcattt aaggctactc aaggtgtagt agctttggta
     3901 aaaagtatgg ataaaagtaa taaaactagt caacaacaaa aagttaactc agaacaattt
     3961 gaccttggta atgaagagga aaataaaaag aaaaaatata ctgtatttat agacccaggt
     4021 catggaggca atgataaagg aactgaaagc aagaccagta atagatatga aaaagaccta
     4081 aatctacaaa ttgcaaaaaa attagcaaat aaattatcta aacaaaagga tattcaagta
     4141 gtagtatcca gaactgatga tacatatata tcattaaagg atagagctat cttagcaaat
     4201 aatagttctg ctgatgtgct tgtttctatt catttaaatg cagagaaaaa tggtaataca
     4261 gcaacaggta tagaaacttg gtataggaat aaagctacag atggttcaaa agaacttgca
     4321 cagacagtac aaagtactat agtatcctat gttaaagtta gagatagggg aatagttgaa
     4381 aataactttg aagtacttag agaatcaaat atgcctgcta tattaataga gtgtggtttt
     4441 ttaacaacac cttcagagga acaaaaaata ataaatgaaa aatatcaaga ccaacttgcg
     4501 gaaggaattg ttcaaggtgt actttcatac ctagattcta aagggaataa gtaagttaac
     4561 tttactttaa tgtgaggata aatttaaaag tttaactgtg taaattgtat taactttatt
     4621 tcgtaatgtt ggaaacattg tttgaattta gaaactatga aattagaaat tattttatct
     4681 atatctaatt tcatagtttt ttttgcaaaa aatttatagt tataaaattt aaaaaaataa
     4741 atttagtata atgtaatgta gttatttata tgtgatttta acttgcattg atttgctatc
     4801 aattgtgctt tataattagt atatataaag tttaactgta agataattta aaaaataggg
     4861 gaatttatat gaataaaaat agcatgacaa aaatttacag tttaaataaa ataattttta
     4921 ttataattgg aattttagga atagttttac ttacaagaca tattgaggta gatattaaag
     4981 ttttaatttt aattttatta actttgttta gtataaatgt atttatttca tatcttaaaa
     5041 ttaagttgta tgaggataaa attaatttag gattgataaa taattcagat aagtttataa
     5101 atattactaa agatgagaac ttcctaaaac atgttcaaaa ttatattatc agtaatgaaa
     5161 aagaaaactg tgtaatggct tgttttgatt tatgtagact caaacttata aatgatatat
     5221 atggttatga gctaggggat gaagtgttaa ataatatttt aagtaactta aaaaattatt
     5281 ttggaaaaga agctatttat ggaaagttaa aaagtgatgt ttttgtatta ataataaagc
     5341 ttgaaaatag ggaacagaag attccttatt tagtaaatct tatcaagaat aaaattatga
     5401 tattaagtca gattgacagt tttaaaatgg atataaatat tgaggcttct attggaattt
     5461 ataaatttaa caataatgaa attgatataa aaaaagctat agataatgct gatatggcaa
     5521 ggcttaaatc aaaaggacta aagcacatag agtatgtaga atttgataat gctatggaag
     5581 aagaaataca atcaattatg aaaatagaga gagatttatt cttggctatt aaaaataaag
     5641 agtttgtaat tcactatcaa cctaaagttg attcaagtac aggtaacatt ataggttcag
     5701 aggcattgat aagatggttg cacccatcat taggcatggt tggaccaaat aagtttatac
     5761 ctatagctga aaaaaatggt ctgataaaca atattggtag atggctaatt caagaagttt
     5821 ttattaccat taacaagtgg atttcagaag gtataaatgt cttaccagtc tcaataaatc
     5881 tttctagagt agaactatat aaaaatgatt tagttgattt ttttaaactg atgtttaata
     5941 catataatat tccaaaagaa cttatagaat tagaaattac agaaacgaca gcgctaagag
     6001 atgtaaaatt tataagtgaa aggctttatg agattaaatc tttaggtatg aatatatcat
     6061 tagatgattt tggagtaggt aattcaaatt ttatcaatct taaggggata ccactagata
     6121 ttataaaaat tgataggtca cttatattgg atatagttac aaatacaaaa acaaagttta
     6181 tggtgaaagc aattattaac ctttcacatg atttaaacgt aactgttata tgtgaagggg
     6241 tagaggatat gcatcaggtc aaagtcttaa gtgaactggg atgtaatttc atacaaggtt
     6301 atgtattttt taaacctttg gatgagatga attataaaaa acttcttact gacggaagtt
     6361 atataaatct gaaagatact cttttagata aatgcatgac agttgaggag gagtaaggat
     6421 gatttcagaa gatgtagatt attcaataag aatcataaga gctatattta ataatggaga
     6481 agataaacca atagatgcta aaatgatatt aaaaagtgaa gatataccaa tactaacact
     6541 atacagactg cttagaaagc tttgtgcaaa caatattctt acttttaaca aagaagatgg
     6601 caagacatac atgttaaatg tgtcattgga tacaataagt ctaagaacta ttgtagaagt
     6661 aattgatgga aaaatatata taaataaatg tacgagatat gtatctaatt gcagtaattg
     6721 cttaaaaggt tgtaaagtaa atcgtgaatt gctagaaata gaaaaagaaa tttataaact
     6781 tttatcgaga aaatctataa gacaaatact agaataaaaa attttcagct atgataataa
     6841 ttggaggttt tcggttatgg atgatatatc tcaagataat tttctactgt ctaaagagta
     6901 tgagaatagc ttagatgtag atactaaaaa agcaagtgga atatattata ctcctaaaat
     6961 aattgtagat tatattgtaa aaaaaacttt aaaaaatcac gatataatta aaaatccata
     7021 tccacgaata cttgatattt cctgtgggtg tggaaatttt cttttagaag tatatgatat
     7081 tttatatgac ttatttgagg aaaatatata tgaattgaaa aagaagtatg atgaaaatta
     7141 ttggactgtt gataacatac accgtcatat attaaactat tgtatatatg gtgctgatat
     7201 tgatgaaaaa gctataagta tattaaaaga tagcttaaca aataaaaagg tagtaaatga
     7261 tttagatgaa tctgatataa agattaatct attttgttgt gatagtctta aaaagaaatg
     7321 gcgatataag tttgattaca tagtgggtaa tccaccctat attggtcata aaaaacttga
     7381 aaagaaatat aaaaaatttc tcctagaaaa atatagtgaa gtttataagg ataaagctga
     7441 cttatatttt tgtttttata aaaaaattat agatatttta aagcaaggag gggttggcag
     7501 tgttataact ccaagatatt tcttagaaag tctatcaggt aaagatttaa gagaatatat
     7561 taaatcaaat gtcaatgttc aagaaatagt ggatttttta ggtgcaaata tatttaaaaa
     7621 cataggggta tctagttgta ttctaacttt tgataagaaa aaaacaaaag agacatatat
     7681 agatgtattt aaaatcaaaa atgaggatat atgtataaat aaatttgaaa cattggaaga
     7741 acttctaaag agtagcaaat ttgaacattt taatataaat cagagattgc tatctgacga
     7801 atggatactt gtaaataaag atgatgaaac tttttataat aagatacaag aaaaatgtaa
     7861 atatagcttg gaagatatag caataagttt tcaaggaata attacagggt gtgataaagc
     7921 gtttatttta agtaaagatg atgtaaagtt gaaccttgta gatgataaat ttttaaaatg
     7981 ttggataaaa agtaagaata taaataaata tatagtagat aaaagtgaat ataggctaat
     8041 atactcaaat gatatagata atgagaatac aaataaaaag atactagatg aaattatagg
     8101 gctatataaa acaaaattag aaaacagaag agaatgtaaa agtggaataa ggaaatggta
     8161 tgaacttcaa tggggtagag aaaagctttt ctttgaaaga aaaaagataa tgtatcctta
     8221 taaatcaaat gaaaatagat ttgctataga ttatgataac aatttttcaa gtgcagatgt
     8281 atattctttt tttataaaag aagagtattt agataaattt tcatatgaat atctagttgg
     8341 aattttaaat tccagtgttt atgataaata ctttaaaata actgctaaaa aaatgagtaa
     8401 aaatatatat gattactatc ctaataaggt tatgaagata aggattttta gggataataa
     8461 ttatgaagag atagagaatt tatcaaagca aataatatca attttattaa ataagtctat
     8521 agataaagga aaagtagaaa aattgcagat aaaaatggat aacttgataa tggattcttt
     8581 aggtatataa gcagttgtaa gtattaaaaa attataatcc tttaattgtt tatttaaatt
     8641 tagaaataaa aagttagcca tataaaaaat attaaaaata tatacttttc aatatttttt
     8701 tgataaaata agaatgaatt tgtgcaattt tatttttctc caataactaa aaatagtttt
     8761 ttgaattaat atttgcttaa gaataaaaat cagaaacgtt tttgatatag taggtgataa
     8821 tatgaaagga ttagttttag aaggtggagg aacaaaagga gcctaccaaa taggtgcata
     8881 taaagcttta agagagcttg gtatagagtt tcaaggagta actggcacat caattggtgc
     8941 tttaaatgga gcatatataa ttcaaaatga tattgaagta atggaagata tttggttaaa
     9001 ttatgactat actcatttta tgaatattga tagagaaacc tatgagaggt ttaaaaatgt
     9061 agatttcact actaaaaatc tcaatgcagt gtttgagctt ataaataaag ctagaaaaaa
     9121 tgaaggtata gatataagcc cattaagaca actaatggag acaacattaa atgaagacat
     9181 aataaggaaa tcaaaaaaag actttggact tgttacggtc tggtgggata aaaaaattaa
     9241 tccatatcca ttgtatatcg aagatatacc taaaggaaga ttgattgatt atttaattgc
     9301 tagttccagt ttgcctattt ttcaaacgaa catgatggat gataagttgt atcttgatgg
     9361 aatgttttgt gataacatgc caataggact cttggaagac aaaggttatg aagatgttgt
     9421 tgtaattagg ttgattgatg attttattgg gaaaatgaat ctaaatcgac atcaaaatat
     9481 aaatataaaa actataatac catcagaata tctaggaggt tctcttaata aagatagaga
     9541 taatgtagaa aaaaacatcc gactaggtta cttagatact atgaaagcat atgaaagatg
     9601 cactggaatt aagtattatt ttgatgtaga gtataaatat aatgaagaat attgttttga
     9661 taaatttaga agtatgagca atgaaacaat acaatatatc tgtaatttat tgaatataaa
     9721 aagagatgct tcacttagaa ctgttatgga aagtgtaata ccaaagcttg gtgaggtgct
     9781 tggattgcca aaggactttt catataaaga tttgttttac tgtatatatg aaaagaaact
     9841 tgaagaaaat aatataaaca ggataaaatt atatgatttt aacaaggtta tagatgttgt
     9901 aaatacaaat attgattcta atagaaattt agcttctaat tttgaagtta gacctaaaat
     9961 tagtattaaa aagaacaaaa acacaaaatt attgacaaat tgtattataa aagacataaa
    10021 aaatcaaagt taaataaaaa acaaaaaaaa atttgtaatt atactcgaaa aaagtgtata
    10081 ttagtagtat aatatttttg tagcactagt gggaacaaaa aatgaccata ggacaaaaaa
    10141 gtaccactta gtaccattaa agtaaaaaac aggaaaacaa ttttaaaaaa tataaaatgt
    10201 aaagtttata ggaggattta aaaaatggaa aaagtagtta gtatcaaaaa tgcaagtgga
    10261 ttacatgcta gaccagcagg aatgtttgtt aagaaggcag ctgaatttaa atcaacagta
    10321 gaggttgtag caaaagacaa aactgtaaat gcaaaatcaa taatgggaat aatgagttta
    10381 ggtttagctc aaggagaaga attaaagtta gttgtaaatg gagaagatga agaagtagct
    10441 ttagctgcaa tggttgaatt agttgaaagt ggatttggag aatagatata ataatttttt
    10501 aaaatttgat atttagaatc taatattact taaagtaaag taacagagaa gtattaaaca
    10561 tatacagacc aacatttgta gatatttaat ttctttgtta ctttcatgtt tatttaatca
    10621 gaagattaag actattgtta attgggtata aaaatataaa tattttagaa aatgaaataa
    10681 gatattttta atcataaatt cagagtaaaa atacagtaac aaacagttta taaaatttaa
    10741 taaaaaatta taagtaaata aaactataaa aatgtaaatt atttatataa atttatagag
    10801 tttatgtgct agatgatatt ttttaggagg tagggttatg gcttacaaag gaataggtgc
    10861 ttctcctggt gttgctttag ggaaagcgtt agtagttgaa catagtgaat tagtaattga
    10921 aaaaaaatct atagataatg ttgaagctga aattgctaaa ttagaagatg cagttgcagt
    10981 ttctaaagaa gaattagtaa aagttaagga aaaggcttct gaagaattag gagcagagga
    11041 agcagaaata tttgaagcac atttattagt tttagaagac ccagagttaa ttggttctgc
    11101 aatagataaa attaaaactg aaagtgtaaa tgcagagtat gctttaaatg aaataaaaga
    11161 gatgtttgtt agcatgtttg agtctatgga caatgagtac atgaaagaaa gagcggcaga
    11221 tataaaagat gttacaaata gaatacttag acatatatta ggaattaaag ttgttgattt
    11281 atctgcttta agcgaagaag ttgtattaat agcacatgat ttaactccat cagacacagc
    11341 aactatgaat aaaaaaatgg ttttaggatt tttaactgat ataggtggaa gaacttctca
    11401 tactgctatc atgtctagaa ctttagaaat agctgcaatc gttggattaa atgatataac
    11461 ttcaaaagtt aaggatggag attttgtagt tttcaatgga gacacaggtg aagtaattgt
    11521 gaatcctgat gaagagacta taaataaata tactgaatta aaagctaagt atgaagatga
    11581 gagaaaagca ttacagctat taaaaggaaa accatctgta actttagatg gaaaacatgt
    11641 ggaattagct ggtaacatag gaacacctaa cgatatagaa ggtctaataa agaatgatgc
    11701 tgaaggtgtt ggtctttata gaacagaatt tttatacatg gatagagatt ctttcccaac
    11761 agaagaaata caatatgaag catacaaagc tgtattagaa ggaatggatg gaaagccgat
    11821 agttataaga acattggata taggtggaga taaagaatta agttatttaa gtatggaacc
    11881 tgaaatgaat ccattcttag gttacagagc aataagactt tgcctagata gaaaagatat
    11941 atttaagact caattgagag ctttatatag agctagtatt catggaagat taagaatcat
    12001 gttcccaatg atttcatctt tagaagaatt gttacaagct aaagaagttg taaaagaagt
    12061 tttagcagaa cttgattctg aaggtgtggc ttatgctaaa gatgtagaaa ttggtatgat
    12121 gatagaagtt ccatctgctg ctgtaatatc tgatgtattg gctaaacacg tagatttctt
    12181 ctctatagga actaatgatt taattcaata cacttgtgca gttgatagaa tgaatcaaaa
    12241 gataagttat ctttacaatc aatttaatcc agctgtttta agacttataa agactgttat
    12301 agacaatgca cataaagaag gaaaatgggc aggtatgtgt ggagaatctg ccggagacca
    12361 aaaaatgata ccaatacttt taggtatggg attagacgag ttctctatgt ctccaatatc
    12421 aatacttcct gctagaaagt taataacttc agttaaagag tcagatatga aaaaattagc
    12481 tgatgatgta cttaatatgg gtactgctga agaaataaaa agctatatag aaaagacttt
    12541 taatatataa ttaatgttat atagttacta aaattcattt ttctaaataa caaaattata
    12601 ttttacgaat taaaaaggag atgtttcaaa ataaaaattt gaagcatctc ttttttataa
    12661 aagattacta agaattactt aatctttgta atagtcattt tgtgaaaaag caaaactgca
    12721 ttgtgactat agtatatatg gatgttatta tcaataaaag tactattaaa atgaagattc
    12781 attctcaaat gatatcaaaa gtagttgtga tggagtaaga taatattttg tgatttaata
    12841 gtaaaaatta aaaaaatatg gtattgaaaa ataattaaaa gtatttaata atagaattag
    12901 ggaagaataa gtttataagt aaaaataatt gattactagt taataaaata aatgtgatat
    12961 ccaaataaat cctaaatcta atttgagttt taatatagat agtctaaagt ttgattatta
    13021 aagatttaat tgtaagttgt tacagttgat gatattgatt agagattaaa gtttagagta
    13081 gaaagttacc ttaaatttaa aaatgcaaga taacccaatc taaaaattat aatcactcga
    13141 taggtttaag gatttatttg tttattataa ataggagggt gtctcaaaat gaacttttta
    13201 gtttatgagg cactctcttt tatatagaat taaatatatt ttattatttc aacaatgaaa
    13261 aagaagctta tctctatttt gagacagcct ctttgtatac tattattaat ttaattaata
    13321 atataattta aaattataat attagttaag atttcctgct ttattagtaa ataattcttt
    13381 aatagcattg tttctggaaa ctaaatcgtt atcagagttg tttgtttttt tcataatcac
    13441 tgaataacgt tttttattta taaaaaatat taaaatctta tcatcatcaa aattgtattc
    13501 tttatatctg tcaatagact cataacctag atttagaatg tttttgtctt ttatactatc
    13561 tacatctaaa atatatgatg ggtcagcctt tagtaatata ttaattaaac atttttcttc
    13621 accagcacag aataactcca tcttacttga tatagattta gattgctctg aagttatttt
    13681 tgcaggaata aggttgtcat tatcatctct tagaagataa ccatgtgtgt cttgttgata
    13741 tactaaatta acctttttct taggaacact aatcatttta tctaaatgta cataatgatg
    13801 attttcaaca aaaccttcat ttaatatttc aacaaagtta ttaattttac ctaggcaaaa
    13861 agattctata gattctttac agaatttatt tgtaaatggt attatgttta cgttttcctc
    13921 atctgtattc catgctatag cataatccaa cgaatatact tcttcctcta tatctgcaaa
    13981 attatattgt actatgtttc ctttcaaaat tactcgacct ccatcttata atatatttaa
    14041 tatactttat aaaacttata ttatatttaa ataatatcat aaattggata aatatgatat
    14101 agtaaataag aaaaaatttg aaaaaaatca ctataaatga gtaataaata taaatagtgt
    14161 atactaatgg gatggaaaat gtccactatt aactttatta ccccacatat ataagataaa
    14221 attattaata taaaatataa tctatcgaca atgaatagac ttttaaataa acttaatata
    14281 aaattaatta aataagtgct taaaaacgat ttactttaat gatagaatca tattaaagga
    14341 ggataatttt atggatataa agaataaaat taaatctata tttttaccag aaaaaaatga
    14401 ctatttagat gatgaagaat ttgagtatga tatagaaaat aatgaagaag atttaaagga
    14461 agataacaaa gtacatttaa atgatttaag cagagtaaaa tatgattaca gagagttaaa
    14521 agacatagaa aaccaaacag aagagatgtg tctgctagca gtaaagcagg atggtactat
    14581 aattgagttt gtaaaggaca agacttataa agtatgtatg gaagcagtaa aacagacata
    14641 taaagctctt aagtatataa caaatcaaaa tgaggatatt tgtatagaag cagtaaaaca
    14701 gaactacaga gctttgtact acataaataa taagacagaa aatgtactaa ttgaggctat
    14761 aaaaaatgca tcaacttatg atgtcatgga agtatttaaa tttgtggaag atcaaacaga
    14821 agatgtatgt ctagcattta tagaaagggc atctaaaaat gatgttgcag agatattaaa
    14881 aggaataaaa gagcaaactc cagccatatg tttagaagct gtaaaaaaag atggaaaatc
    14941 tttagcttat gtaaaggaac aaagtaatag tatatgttta gaagctgtaa aagaaaacta
    15001 cagtgcacta tcttgtgtaa aagagcaaac agaagaaata tgtatagaag cagtaaaaca
    15061 gaatgacttt gcattgtatt atgtaaatga acagacagaa aaaatatgta tggaagcagt
    15121 aaaaagaagt tatatggctc tacagtatgt aaataagcaa acagaagaga tttgtttgga
    15181 agcagtaaga attgatggaa gagcattaca gtacgtaaaa gagcaaacag aagaaatttg
    15241 cttggagtca gttagacaaa atggaaaagt attgcaatat gtaaaaaaac agactgaaaa
    15301 tatatgtata gaagcagtaa gaggttcatt tgaagaactt gaaataaaag aaatattaag
    15361 ttatgtaaag ataccaacag aaagaatatt tgtagaagca gtaaagcaaa atggaaaaat
    15421 tcttaaatat gtagaaaatc agacagaatt aatatgttta gaggcagtaa gagaaaacta
    15481 taatgcttta gcttatgtaa aggaacaaac agaagagatt tgtttagaag cagttaatca
    15541 aagttatgaa gcgcttaaat atgtgaaaga acaaacagaa gaagtttgtc taaaagcagt
    15601 aaaacaagat tatagaatgt tgaaatatgt aaataatcaa acagaaaaaa tatgtttaga
    15661 agcagtaaag caaaattata gagctttaga atttgttgat aatcaaacag aaaaagtttg
    15721 tcttgaagca gtaaaacaga atagaaaagc actacagtat gtaaaacaaa aacaaagtta
    15781 aaaggctaaa atatatagaa aatatatgca aataaataaa atttgatatt ttgtaataaa
    15841 taaaatgggt atctcaaaat gaacttttta gtttgtgagg tactcttttt atatgaaata
    15901 aaatatattt ttagcctttt aacaatgggg aaaaggttta tttctatttt gacataggct
    15961 ttttatgcat ataaagcttg ataaaattgt tttattataa ttgttttgtt atataaataa
    16021 taataagaaa attatatagg tctttttttg accagttttg catttttaac aactagtttt
    16081 gtaaagtata ttgaattttc tcaaaaaaga atgtactcta tatatgtaaa atttttttgg
    16141 aggtaaaaat gtttaaaaga tatgctgaaa aaatgacatc tgttttaatt tgtaataata
    16201 tgatagataa taatgaatct aaagtctatt cttatggatt tgaaatatta atagctttca
    16261 tagttaatat aaccaccatg ctgtttatag gttttttatt tggtaaattt acatatgtat
    16321 tatttttttt aatgtgttat tgtccaataa gacaattttc aggagggtat catgcagaca
    16381 attattttag atgcttatta acctttattt ttataatttt atctacaatc ttaattatag
    16441 aaaatataaa tatagactta tttaaaaata ttattatgat tattgcaagt gttagttggg
    16501 ttggaatttg tgttctgtgt ccaatagaac ataggagtaa ccctataagt gatagggaaa
    16561 agtttgttta taagaaaact gctatattta tatctacagt agtgttattg ataacaattt
    16621 taagtctttc tttgagtata tttgtagatt atttcacata tagcgcattt gccatgtttt
    16681 ggatttttgt gatgttagta ttaggtaagt taaaagctaa agtataagta aaatatgtat
    16741 gagggagagt taagttatga aaaagtttat tgtaagattt atgaagtttg ctagctcatt
    16801 ggcacttagc actgctattt tgtcagctaa tagtacctgt ccttggatta ttcatcagcc
    16861 taaagtaccc aaagaaatca gcaatctaaa gaagacaaat taaatacaag cacttattta
    16921 tacaaaatta tcaggaaaaa agaaattatt atattttaat aagattaaaa caatataaaa
    16981 tcagatacaa aatttatttt atttatttta taggagccta atacctattt ggtaatatat
    17041 tagtgcgcca atttaggggg atatagtgta tttaatgaaa tgacttacgg agagtaagtc
    17101 atttttgtat tgtatgtatt ttatgtaaac ttatgatata atgaagggta aagtttaatt
    17161 aaaataataa aaaactttag tatagatagt aatttaggag gtagggtatg agtatgatac
    17221 ataaattttc tatgaatgga tataatatcg ttttagatgt gaatggtgga gctgttcatg
    17281 tgttagatga tgttgcatac gatttgttag atttttataa agaaaaaagt aaagaagaaa
    17341 tattagagat attaaaatca aaatatcaag aagaaaaaat aaatgaagca tatgaagaaa
    17401 tattaaattt ggaaaaagaa ggtcttttat acacagaaga tacatatcaa tatcatccaa
    17461 gctttgtaca tagagagcct gtagtaaaag ctttatgttt aaatgtagct catgattgta
    17521 acttaaaatg taaatactgt tttgcagctc aaggtgattt tggaggagaa aaagaactta
    17581 tgagttttga agttggtaag gcagcaattg actatttgat tgctaattca ggaagtagaa
    17641 agaacctaga gatagatttc tttggtggag agcctttgat gaactttgaa gttgtaaaac
    17701 aactggttga ctatggaaga agtgtagaaa aagattataa taaaaatata agatttacaa
    17761 taactacaaa tggtgtactg ttaaatgatg agattataga ttatataaat gaaaatatgc
    17821 ataatgtagt tttaagttta gatggaagaa aagaagttaa tgataatatg agaccaactt
    17881 taaatgataa aggaagttat gatattacat taccacgatt taagaaactt gtggaaaaaa
    17941 gagcaaaaga taagtattat tatataagag gtacttttac aagagataat ttagattttt
    18001 caaaagatgt tatgcatttt gcagatttag gatttaaatt aacctctgta gagccagtcg
    18061 ttggagatga gagtaatcca tatgcactta gagaagaaga tttacctaag atatttgaag
    18121 agtacgaaaa atttgctgta gagtatgcag atagacaatt acaaggagat ggatttaaat
    18181 ttttccattt tatgatagac ctaaatcaag gtccatgtgt aataaagaga ataactggat
    18241 gtggagctgg aaatgagtat ctttctgtaa ctccaaatgg agatatctat ccatgtcatc
    18301 aatttgttgg aaatgaagaa tttaaaatgg caaatatatt tgatgaagaa atagtacttc
    18361 cagaaaattt aaaaaatatg tttagagaag ctcatgtata tactaaagaa gagtgtaaac
    18421 aatgttggaa taagttttac tgttcaggtg gatgtcatgc aaatgcgata aactttaata
    18481 atgacatatc aaaaccatat gaattaggat gtgaaatgca aagaaaacgt acagagtgct
    18541 ctataatgat acaagctaag ttaatgttag aaggagcaac caactaaatt tatattttaa
    18601 aggagttgtt tatatatgat tagagattat ttagaagata aacctttaat tgacgaaagt
    18661 gtatttgtgg caaaatcagc agatgtaatt ggaaatgtta aaataggcaa agattcaagt
    18721 atatggtaca atgcagtagt taggggtgat gaaggaccaa taactattgg agaaaacact
    18781 aatatacaag attgttctat agtgcatgga gatacagaaa ctataattgg taacaatgta
    18841 acagttgggc ataggtctat agttcatggt tgtaaaatta gtgataatgt gttaattggt
    18901 atgggttcaa taatattgga taatgctgaa ataggagaat atactctgat aggagcaggt
    18961 acactaatta catctaataa gaagtttcca ccaggtgttt tgataatggg ttctcctggt
    19021 aaggttgtta gagagcttac agaagaagat aaaaaatata ttgatgaatc ttatgaatgg
    19081 tatttagagg cagcacaaaa tcaaaaatat tagtttaaag agtggttgtt tcaaaacata
    19141 tttaaatctg ttttgaggca accactaatt tattttatta gattttgttg atattttagt
    19201 tttcaaaaag tatttgatta aaccagatat aaaatgtcaa aaataaaaat taactgagtt
    19261 aaataatttt aataaaattg gtagtatatg tgataaaatt aaatattaaa gaggatatag
    19321 gatattagaa tatcaattgg tgaaataatg tcatatacga taaattaagg tgtaagtttt
    19381 tgggtatacc tatcttagga gtgaaaagat ttgagaataa gttatagaaa tttaaagcga
    19441 tatagagaaa ttggatatgt tttaataaag tatggtttta gttttattgt agaaagacta
    19501 aatatagaag gaattgccta taaaatacca ctatttgacc caccagaaga aataaaaaat
    19561 atgacaactg gtgagagaat gaaaagagtt ttagaagaac taggacctac atatataaaa
    19621 ataggacaga tattgagtac aagaaaagac ttgttagacc aggatataat tgatgaaata
    19681 tcaaaactca gagatgatgt agagaaattt gattcaaata tagctattga tatatttaaa
    19741 gaagaagtgg gtttaagtat agaagaaata ttcttagaat ttaaagaaga accaatagca
    19801 gcagcttcaa ttggtcaagt ttatgaaggt gtacttaaaa cgggagaaga agtaatcgta
    19861 aaaattcaaa gaccaaatat agagaaaata ataaaatctg atttagagat tcttagaact
    19921 attgcaaata ctttaaaaga tttaaaaaag gattttaatt tagatttagt acagatgatt
    19981 gaagaatttc aaactcaatt aatgagggaa cttgattata catttgaagc cataaatgca
    20041 actaaattct ctaggatatt taaaaatagt gatgaagtat atatacctaa agtatactca
    20101 gaatataata ctaagaaaat tttagtaatg gagaaagtta atggtacaaa gttaagtgat
    20161 gtagaaaaaa taaggagact aggttataac acaaaaacta ttgttgaaat aggagttagg
    20221 tcatttttta cacaggtatt atctcatgga ttttttcatg cagacccaca tccaggtaat
    20281 atatttgtag ttgctaaaaa taagatagca tatatagatt ttggaatgat tggaattatt
    20341 gataataaaa ctttaaacca attaaatgaa attgcattag caggtgtaga aaagaatgtt
    20401 gacaagataa tatatctatt gatagaaatg gatgcattaa atggagaggc tgatattaag
    20461 ggacttagac aggatttact ttatctaata cattattact atgatatttc tatagaaaaa
    20521 ataaatgtaa cagatatatt aaatgaacta tttaggtttt ttagacagta taaaatagtc
    20581 atgcctgcac aatttgtaac tcttgcaaag acagttataa cattagaagg aacaagtaga
    20641 actttaaaca cagatttttc ttttggttct atgggaaaag agtttatgaa acatcattat
    20701 aagagtaaat ttaaccctaa aaatgttgtt ttaagttcaa gacaaaatgt agaagaaata
    20761 ttactggaca taaaaactat tccaaagcaa ttaaaagcaa ttttaaaaaa tatagaaaga
    20821 aataatataa aaatgcaaat tgaagatgtt aaaatgacga ggttagaaaa ttgtataata
    20881 gagctaacat ctcaaatatc tttgagttta gtgttggcat ctattatagt aggttcttct
    20941 cttataattg catctccaaa tatagaaaat aatatatgga taaaatttac tgcaatagcg
    21001 ggtttcttta tatcctttat aataggcctt tgcttagtta taagaagtat taggtcaaaa
    21061 tacaaaaaag attaggggga attaaatttg aaaaaatgga tattgaagta taaaggtaag
    21121 atagaggaaa gtgaatttag caaaaaactt aatatttccc cggaaatctg ccaaatttta
    21181 aaaaatagag ggatatgtac tgaaaaagat tcagaaatat ttatgaatcc atctttagat
    21241 tatttaagag accctttttt aatgaaagat atgcaaaaag ctgttgatag aataaagtta
    21301 gcagttgaga agaacgaaaa aatttggatt tacggagatt atgatgttga tggtgtatca
    21361 tctacatcca tcttgtgttt atattttaag agtataggtt acaatgttga ttactatatt
    21421 cctaatagat tagaagaagg gtatggtata aatgaggatg ctattaagct tataagctct
    21481 aggggatgtg acttgattat aagtgttgac tgtggaatta catcagtgtc tgaggtcaat
    21541 acagcgaatg atttgggcat agatgttata attacagacc atcatgaatg tcaaagtgaa
    21601 attccatcag cctttgcagt aataaatcca aaacaagaag attgtaatta tccatttgat
    21661 tcactatgtg gatgtggtgt agcatttaaa atgattcaag ccttgactcc aaaagaagaa
    21721 tttaaaacta gtatgtataa ttatttggaa atagtaacac tagcaactat ttgtgatatt
    21781 gttccactta tagatgaaaa tagaataatt gtaaaaaatg gtttaaaatc aatgaaagaa
    21841 ggcaaaaata ttggattaag ggagcttata aaagtttgtg gtgtagaaag tgataagata
    21901 ggttcttctc atataggttt tgcaataggt cctaggataa atgcttcagg tcgattggga
    21961 tattcttact taggagtaga gctatttact actcagtcac aagaagaggc agtagaaata
    22021 gctagtatat tggaagaaaa aaataatgaa agacagatga tagaagctaa gatgtatcat
    22081 gaagctgaag aaatgctaaa gtctaatagc agatacaatg atgacaaagt gttggtttta
    22141 gccaaagagg gttggcaaca tggaataata ggaattgtag catcaaagtt aactgaaaaa
    22201 tactacaaac caacaattct tctaggtata gaaaatgggg aggctactgg ttcagcgagg
    22261 tcaataaaag gatttaatat atttgaagct ctcataaagt gcaaagactt gatgactaag
    22321 tttggtggtc atgaacaagc agcagggtta tccttggatt cagacaatgt agaaatttta
    22381 gcaaatgaaa taaataaatt tgcagattat aatttaactg aagatgatat gatagagaat
    22441 gtaaatgtcg agtttgaatt acaagaaaat gtaataaatt taaatcttgt agaagaatta
    22501 cataagttag aaccctttgg attaaataat cctaatccta gatttatagt tagaaattat
    22561 attttaaaag atttgaaagt tataggtaag aatcaacaac atctgaaatt aagtatagaa
    22621 aaagagaaaa gctatgaatg tataggattt aatatgagtc atttaaaatc aatgtataaa
    22681 gtaggagaca aagtagatgt actattccaa ttagatgaaa ataactatat gggaaatagg
    22741 aaagtacaat ttttacttaa agatatacga ttggctcgac ctaaaagtgc aagtaatgac
    22801 aaactttcat tgaaattaat gtcaaaaata ataccaaaag atactcaaag cctgtataat
    22861 atatctgtgt ctgattttga attatttgat ggaaatacag atataaatat atttgattat
    22921 tttgaaaaag atacactaat aatatctaat tctataaatg gattttatag agcaatgtca
    22981 gatatatcac ttatagattt ggattttaac ataaattaca atattattga agatgttagc
    23041 aaaaatactg ataaattaga gcttatattt tctcctaata ttgataaaat agacttaaaa
    23101 aga
//
LOCUS       DAELXX010000063        22976 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.063, whole genome shotgun sequence.
ACCESSION   DAELXX010000063 DAELXX010000000
VERSION     DAELXX010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22976
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.063"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(267..1358)
                     /locus_tag="KPH29_002254"
     CDS             complement(267..1358)
                     /locus_tag="KPH29_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467843.1"
                     /translation="MVMQVQEKILELLYTINEDVVSIKNDMSEVKQEMTVMKEDISEV
                     KQEMTVMKEDMSEVKQEINVLKGDMSEVKQEMTVMKEDMSEVKQEINVLKEDMSEVKQ
                     EMIVMKEDMSEVKQEMTVMKEDMSEVKQEMTVMKEDMSEVRQEINIMKEDISEVRQEM
                     TVMKEDMSEVKQEINVLKEDMSEVRQEINIMKEDMSEVRQEMTVMKEDTSEVKQEINV
                     MKKDMSEVRQEITVMKEDVSEVKSEVNFMQNKINNINEDMNGIKDEVSIANEKLYGIE
                     IKIDSLECEDKSMKEIQIEQNNILGSLLHNSEINKATHDNIEHNIAYIKGDTNSIKED
                     IAEIRRDLNLVELATSKNWSDIVKLKSVK"
     gene            complement(1588..2673)
                     /locus_tag="KPH29_002255"
     CDS             complement(1588..2673)
                     /locus_tag="KPH29_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBG1467844.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASVGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            complement(2709..4022)
                     /locus_tag="KPH29_002256"
     CDS             complement(2709..4022)
                     /locus_tag="KPH29_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG1467845.1"
                     /translation="MIAIKSKYLISSADEIYTNSAAVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLDENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDAKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAYVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            complement(4023..6314)
                     /locus_tag="KPH29_002257"
     CDS             complement(4023..6314)
                     /locus_tag="KPH29_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG1467846.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDTKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGADKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGRTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            complement(6304..7098)
                     /locus_tag="KPH29_002258"
     CDS             complement(6304..7098)
                     /locus_tag="KPH29_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBG1467847.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLESELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMAKALVVRMYNSIGGECDGK"
     gene            complement(7137..7583)
                     /locus_tag="KPH29_002259"
     CDS             complement(7137..7583)
                     /locus_tag="KPH29_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBG1467848.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVDGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            complement(7655..9061)
                     /locus_tag="KPH29_002260"
     CDS             complement(7655..9061)
                     /locus_tag="KPH29_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG1467849.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTANEEKALKDAQDTAERLC
                     VRGTIKNRMEGHKWNSLY"
     gene            complement(9079..10449)
                     /gene="hydA"
                     /locus_tag="KPH29_002261"
     CDS             complement(9079..10449)
                     /gene="hydA"
                     /locus_tag="KPH29_002261"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBG1467850.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEKIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKMAREKNIDMIVETCPQYLLLEDNLYNKN
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISVNKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            complement(10582..11769)
                     /locus_tag="KPH29_002262"
     CDS             complement(10582..11769)
                     /locus_tag="KPH29_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HBG1467851.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGVNAVYKMANVIQKIQQLVPPT
                     HPVLGDGILVLTDIKSSPYPGASVVPDYCKATFDRRLLVGETREGVLAPIQALLDEMM
                     KEDPELNAKVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDSE
                     ITQYSFCTNGSHYAGEAGIKTIGFGPSKENLAHTIDEYIEQEQLFIGTEGYYGILKSV
                     YGK"
     gene            complement(11896..13110)
                     /gene="dpaL"
                     /locus_tag="KPH29_002263"
     CDS             complement(11896..13110)
                     /gene="dpaL"
                     /locus_tag="KPH29_002263"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBG1467852.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            complement(13473..14144)
                     /locus_tag="KPH29_002264"
     CDS             complement(13473..14144)
                     /locus_tag="KPH29_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG1467853.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            complement(14227..16443)
                     /locus_tag="KPH29_002265"
     CDS             complement(14227..16443)
                     /locus_tag="KPH29_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG1467854.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHNIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFRGSLGNPRVLDCIEQGKKMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLIVEEAEKLLETSKEDLYLDNGYVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGLTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVNSYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            complement(16446..16916)
                     /locus_tag="KPH29_002266"
     CDS             complement(16446..16916)
                     /locus_tag="KPH29_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HBG1467855.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIKEAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            complement(16978..18372)
                     /locus_tag="KPH29_002267"
     CDS             complement(16978..18372)
                     /locus_tag="KPH29_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG1467856.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQLAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            complement(18445..19806)
                     /locus_tag="KPH29_002268"
     CDS             complement(18445..19806)
                     /locus_tag="KPH29_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBG1467857.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTVIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            complement(20104..21426)
                     /locus_tag="KPH29_002269"
     CDS             complement(20104..21426)
                     /locus_tag="KPH29_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBG1467858.1"
                     /translation="MFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVVGGAVGVSPT
                     QITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVGGNYGLASIL
                     GAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMAGGVNSPTYA
                     SMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIINFDPIREATW
                     FAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNITDKELSSGVI
                     GSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGFVPKFGALM
                     TTIPYSVLGGATISIFSMITMTGIKLIITEELSSRNITIVGLAIALGMGITSVPAAQE
                     AFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            complement(21782..22822)
                     /locus_tag="KPH29_002270"
     CDS             complement(21782..22822)
                     /locus_tag="KPH29_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG1467859.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
BASE COUNT         6839 a         4647 c         2527 g         8963 t
ORIGIN      
        1 ttgtatagta taaaattttt atgttctcat tgataaaatt ttacttattt caagcgtttt
       61 attcgatttt atcatctaaa ttttaggttt tatttttata aattaattgt atttacataa
      121 aaaaaaagac ctaaaaacaa atttttaacg gtcttttctc aattttttat tttcatttct
      181 aaaatttata ataaatagta agaacactat aaattctttt atataaaatt cttagtttgt
      241 ggatgataaa ttagtgaatt aaaatactac tttactgatt ttagtttgac aatatcactc
      301 caatttttac tagtggctaa ctctactaaa tttaagtctc ttcttatttc agctatatcc
      361 tcttttatag aattagtgtc accttttata tatgctatgt tatgttctat attatcatga
      421 gtagctttat ttatttcaga attatgtaat aaacttccta aaatattatt ctgttctatc
      481 tgtatctctt tcattgattt atcttcacat tctagactat caatcttaat ttcaattcca
      541 tataattttt catttgctat ggatacctcg tccttaattc cattcatatc ttcattaatg
      601 ttatttattt tattttgcat aaagtttact tctgatttaa cttcggaaac atcttctttc
      661 attacagtta tttcttgtct tacttctgac atgtcttttt tcattacatt tatctcttgt
      721 tttacctctg atgtatcttc tttcattaca gtcatttctt gtcttacttc agacatatct
      781 tctttcatta tatttatctc ttgtcttact tcggacatat cttcttttaa tacatttatc
      841 tcctgtttta cttccgacat atcttctttc attacagtca tttcttgtct tacttcggat
      901 atatcctctt tcattatatt tatctcttgt cttacctctg acatatcttc tttcattaca
      961 gtcatttcct gttttacttc cgacatatct tctttcatta cagtcatttc ctgttttact
     1021 tccgacatat cttctttcat tacaatcatt tcctgtttta cttccgacat atcttctttt
     1081 aatacattta tttcctgttt tacttccgac atatcttctt tcattacagt catttcttgt
     1141 tttacttccg acatatctcc ttttaataca tttatctcct gttttacttc cgacatatct
     1201 tctttcatta cagtcatttc ttgttttact tctgatatat cttctttcat tacagtcatt
     1261 tcttgtttta cttccgacat atcatttttt atgctaacta catcttcatt tatagtatat
     1321 aataactcta aaatcttctc ttgcacttgc atcaccatcc ctattaatta ttataacatt
     1381 ttacacaaat gctatacaat gttaacatat atatcagaat tattaaaatt ttaattaaac
     1441 aaaattgatt cactttatta cttcaaaact aattaatatc taaaataact acaaataaac
     1501 aaaaataagg tcaacaacaa aaaaggctta tctctttgag atagcctttc ttatttttat
     1561 ttaaaacttt gttttttata aaataatcta tacattcagc tctgaacatg aaatttttct
     1621 tccaagtgct tttggtttac aaactgatat acaaagtcca caatatctac actcttcttc
     1681 attcaattcc atcttatcat tcaattttct cgcttgatat gcacaaactc tctcgcacat
     1741 cttacattta gtacattttt cttcatcata agtaaactca aagctaagtc tttcattaac
     1801 atctcccata tgattatgta ctataccaca agcattttct atagtattat atcctaatct
     1861 attaagatta gattttaaat catcatgtat tttagatatt acttgtgcgc cttttaaaat
     1921 tactactgaa catataccaa caaagtcagc acctgccata atcatttcta aggcatcatc
     1981 tccacttaca catccaccta agccaattat tggtatatta acttgttttt ttatttcata
     2041 tattctctgt aaagttattg gcttaatagt ttctccactt aaatatccta ttccatttcc
     2101 acctaataaa ggttgccctg tttctatgtc tattctaaat gctggtccta cagagtcaca
     2161 agcagttatt gcatctgctc ctgcttcttc taatttttta gcaaaatcgc ctatctcatc
     2221 tatcatagga ggaagtttaa ctattacagg tatatttact ctttcctttg catctttaag
     2281 cattggtatc aaatctctat agtcataaga tactaattca ataaagtcag ctcctgcatt
     2341 tgcaactttc tctactaatt cgctactttc ttcaattgta tgacctactg aagctataca
     2401 gacagtccca tctcttttca tttgtggtat ttcaaaatct atccattgct ctggctcaaa
     2461 atctgtccat ttttcattat ttaaaagagc attagctgta tttctaacca tatgtggtgc
     2521 tggatttata gctctttctt ttcttatagt cttagtcaca actgcccctg ctcctgcatc
     2581 acttaatatc ttgcatccag ctgcactata tgttaatggt cctgaaccta ttataagtgg
     2641 tgattttaat tctttaccta aaaaattata cattatctgc ttcccctttt catatatatt
     2701 tacattcgtt aaaatttcca aaattcacaa gcaacttctt tcatttcttt aacaatagct
     2761 tttttatcta aatcaactaa ctgtttttct tttaaaatat gtctaccatc tatgtataca
     2821 tttgatataa attctttttt accctgaact actatctggt caaatatatt atcaagagtc
     2881 acaggagttt taaattcatc ttccataact attatatcag ccttattacc aactgctatt
     2941 ttaccagaat tttgaagtcc taatacataa gcaccatgct ctgttgccat cctaaatact
     3001 aaatttgctg gcattactga tgcatcttct tcaactgatt tatgcattaa aaatgcacct
     3061 ctcataactt caaaaaaatc atttatatat ccatcagtac ccagtgccac ttttatgcct
     3121 tttttaatca tttttggtac tggagaaaaa cctccaccta cttcacagtt gctaagaggc
     3181 atatgaacca cttttacacc tttttctttt aatatatcta tttcttcatc atttactttt
     3241 acacactgag atgctaatac agtttcatcc aatatatcta aatcattata gtagtcaact
     3301 gcttttttgc caaaatgttt ttcagcatag tttggctcat atatactctc acataaatga
     3361 aattgcattg gtgcatttaa tttttttgca tcttctttag ctttcttaat aaatctatca
     3421 gaacatgtga agcttgtatg tgtacacatt attccattta ttaatttact attttctcta
     3481 ctccacttta ttaaattaga attttcatct aaacatctaa gtccattttc aaagcttatt
     3541 ctttcacaac tttctaagga taaaatcgct ttcatgccta tttcttctag tatttttcct
     3601 tgctcaatta aagttccttc ttctgtattt ggagcttcta gggtatcaca aaaagctacc
     3661 acaccagatt ctataagttc tattgctgat gctttagttg ttgccttcac ttctttcaat
     3721 ccaattctat tttctataaa tggccaccaa tagtcattta aaaaaccttc aaagtcagta
     3781 aaatgtacat ttgccggtat tcccctagaa agaactccat attgatgcat atgaccattg
     3841 acgaatcctg gcattattat agcatctgat ttatctatta tttcttcaat atttttatat
     3901 tttatttcaa cttcttcatt tggcaatata tcttttataa tgtcattttc tactacagca
     3961 gcagaatttg tatatatttc atcagcagaa gatattaagt atttactttt aatagcaatc
     4021 atttatttac ctccctgatt taattttttc atggccatat aaactttctc agaagttata
     4081 ggaagttctc ttatacgaac tcctgtggca ttgtatattg catttgctat tgctggtgca
     4141 ggtgtatgaa taactatttc tcctaaagat ttagctccat atggacctgt tggctcataa
     4201 ttatctacaa aatcaacaat tatgtttcct atatcttttt ttgttggaac tttgtattgt
     4261 aatagattag aagtttgaag ctttccatca gaaccatgtc tagggtcttc atacattgca
     4321 agccctatac cttgagttac accaccttca acttgtattc tagcaagtgc aggattcata
     4381 actgtaccac aatctgtaga acaagcataa tttattactt taaactcccc tgttgtttta
     4441 tctaactcta tttcagcaaa tcctgctaag aatggtttag cactctcttg tataccataa
     4501 gattcagatg aatataaaac ttcttgattt ccaccaccaa cagattcttt tcctaattca
     4561 cttaacaaaa caaacttctc tacatcttca ctataacaaa ctctgtcttc atatagtatt
     4621 aaatcatcta ttggtaaatc gagtttcttc tgtgctactt ttaatatctt ttgttttaaa
     4681 ctttcagcag attttattgt agcatttcca gttacataag tagtacaaga agcgtatgca
     4741 ccagtatcat atggacacat atctgtatct cctgtataaa cggaaattct atcaatagat
     4801 gtattcaatg cttttgcagc tatttgtgca agtattgtgt cagaaccagt tcctaagtct
     4861 gaagacccag aaaacaactt ataagttcca tcttcatcca tcctcatatt tactattgcc
     4921 atgtctatgt tggctatacc agaaccatga gttcccacag acattccaac agccctcacc
     4981 ttattgtttc caatatccct aacagggtac ttttcatccc atccaataag ttttttacct
     5041 ctaattatac attcatcaag tgcacaactt cttattggaa aattcattat tccgccttca
     5101 gtttgttttt ttataatatt ctttaacttt atttctgttg ggtccatatt aagtttatgc
     5161 gctaattcat caattgcaca atcaagtgca aatgtacctt gtgttgctcc atatcctctt
     5221 aatgctcctc ctggaactaa attagtatat acagttctcc catcaaattt tattgcaggt
     5281 acattattat atgttggaag tacattatga cctgattcag agcaaactga aggtccatta
     5341 tcaccataag cgcctgtatt attcaatgct ttcatatcta tagcttttat gtttccttct
     5401 ttatctgccc caatttttat atcaaaaaac atctgatgtc tagtattagt cattgcaaaa
     5461 gtttcttctc tagtataaat tagttttgct ggtctttttg tcatccaagt tacaaatgat
     5521 gcatatatct ctgttacagc tatgtttttt ccaccaaaac ctccacctat tcttggtttt
     5581 attactctaa cttttgaaag aggtattccc agtgctttag aaacctgtct tctcatatga
     5641 taagctgatt ggtttgcaga agttactact aatctgccat tcgtatctat atacgtataa
     5701 gctctatgag tttccatcat acaatgggct tgtgattgag tcatataaga atgttctact
     5761 attacatcac tttctttaaa ctctttttct acatcccctt tacccatttt ttctctcgat
     5821 acaatatttt ttgtagcatc caatccaaaa tcaaatggac aaaacaaatc atcttcttca
     5881 tggattaaaa ttttatttcc ttccgattct cttgcatcca atataggagt cattacttca
     5941 tattcaactt ttataagttt catagcttta atagcagccc tttcatcttc agcggctatt
     6001 atagcaactt catctcctac atatctaact acgtcttcaa gtattaattg gtcatgaggt
     6061 gatgcttctg gatatgactc tccaaccatt gaatatctat aatttggaac atctttgtaa
     6121 gtgaatatgt ttactacacc ttttaccttt aatgcatttt ttgtatctat gttttttatc
     6181 ttcgcaaaag catgcggact tctaagaagc ttaattgtta aaacatcttt atgaaaaatt
     6241 aaatcttctg tatatattgg ttttccagta acaatggctt cactatctat ttttcttacc
     6301 tctttattta ccatcacact cacctccaat actattatac attctcacta ctaaggcttt
     6361 tgccatatct tttctgtatt ctttacttcc tcttatatta cttccaaatg aaagttcttc
     6421 acttgatact tcaccagctt tttctatatc attttctaaa cttaatattt cacttgcttt
     6481 ataggcaatt tttgctcttc ttggtctagc gcctatcgca attttataaa tacttccttc
     6541 ttttaacatt gctccattta atactgcaaa atcacctgta cattttctta tagaatcaaa
     6601 tacacctata gcatcttttt taggtaatat aacttctatt aatatatctt tttcaagctc
     6661 actttctaaa aaatcagcta agttcataac ccctttttta tataacttaa cctttgcatc
     6721 taccactagt aatgaaggta ttaaatctga aaatccatac ttagcaaata cgctagctcc
     6781 tactctggct ccacttctaa attgaactcc tactatatta gaaacagcgt ttgaaattac
     6841 cccattacag taatttttaa taaccttatt taattctaaa gttcttaaac ttgtgtctgc
     6901 tccaattaat atgctatcat cagtttcatt tatataatct aaagctaagt cttttaaatc
     6961 tattgctgag ccaatatttt tactacctag ttttaaaaat ccacatcctc caagtattat
     7021 gttgtttttt cttgatatta gcagttcata agcttcatct aaactttttg gaactacata
     7081 ttcctttata gttatcatac tctctcccca tatcaactta attttcactt aaaagtctaa
     7141 gtgtttaaat atttatgtat tgctcttaat tgacctttat atcctgtaca tctacaaagg
     7201 ttaccattta cataatctat aacttcttct tctgttggat tttgaagctc tcttttcatt
     7261 gccaaaacca tcattataaa tccaggagca caaaatccac actggtcagc cccttcgtct
     7321 gcaagaaaat ctccaaactt ctttgcttca tccaagaccc cctcaagtgt tgtaatatgc
     7381 tgaccatcta ctctagcagt aagataatta catgaaagca cggccttgtc atttaccatc
     7441 actgtacata acccacatga gcctgtatca cacccttttt taacacttac atatccattt
     7501 tctcttaatg tatctgctaa aaaatcatct ggtcttatac ttaattcttt ttctttatta
     7561 tttatggtac atttgataat caaataaatc acctcaaaac tcatattttt aagctaaagt
     7621 tttactatac ttttctatga ataaattaca tttattaata aaggctattc catttatgac
     7681 cttccattct attttttata gttcctctta cacaaagtct ctcagctgta tcttgagcat
     7741 cttttaatgc tttctcttca ttagctgtca atatttttga atcttccatt attatatttc
     7801 catctactat aacactctct atatttttca tactagaaga atatactaat gtagataccg
     7861 gattatgcat aggtatcgct tttggtgaaa gcattggatt aaatattagt aagtctgctt
     7921 ttttacctat ctccaatgaa cctatctcat cttccattcc aatagctctt gctccatcta
     7981 tagttgccaa ctcaagaact ttttctgccg acattgctaa tgggtcacac ttattaactt
     8041 tatgttgaag tgctgttaat ttcataagtt caagcatatc ttgagagtta ttactagcgg
     8101 ctccatctac tcctaaacta actgttattc cttttttaag catttctggc actggtgcaa
     8161 cacctgaaga taagtacata ttacttgctg tattatgaga cactttcata tcgtattttt
     8221 tagtaagttc catatctttt tcagttagat acacacaatg taccataagc acatttggtc
     8281 caagtattcc taatttttct aaaacatcta tgtcatattg cccatgcaat tcttttgctg
     8341 cttctctatc aaaaggagtc tctgatatat gtactgtaaa taaagcatca tcatattctt
     8401 ttaccactct ccatagcatt tcaagcattt cttgagaatt tgaccatatt gctgcaggag
     8461 ctactcctat tttgattctt ccattttctg tattatggtg tttttcaaac aatcttctta
     8521 catctttttc aaccgtttct acatcttgca ttattccagg atgaactcca aattgagctc
     8581 ctgtattcat acatcctctt ccaagtattc ctcttattcc taattcttta taagcatcta
     8641 ttattccatc acaaagacct ggcttactat gaggatacat gtagtcaacc atggtcgtta
     8701 taccacttct aagtccttca atacatccaa gcattgcagc atcataagta tcttttggct
     8761 ctaaatagtt agctgctgga aatgtcatag tttctaacca gtctttaagt accatatcat
     8821 ctcccaatcc ttttaataaa gtttggaata aatgattatg tgtatttata aaacctggga
     8881 ataaaacttt acctttagca tctataattt ttttaacatc agtatattta ctttctatct
     8941 ctttagaatt tccaatatca gcaatcttgt tatcttttac tactaatgcc ccatcaaaaa
     9001 taacttctct ctctttattc acagttacta ttatggcatt cttaataagt atatccatca
     9061 ttcttatttc cccccgcatt atttagtaat ttttctttta atatattgac ctgttggatg
     9121 ttctttaaca ttacaacttt gtactacatg atttcctctc aaaaatacat cttcaacttt
     9181 acatttaacc ttaaatcctt catatggagt ataatctaca ttttgttttt gagttttata
     9241 actaattgtt tcttctttat tgatatttaa aatgactata tctgcatcac ttcctacagc
     9301 tatctcacct tttttaggat acattccaaa tatttttgca gcattcgtag aagtaacttc
     9361 cacaaactta tttacactta ttctattttc taagacccca tatgtatata aaagtgctaa
     9421 cctatgttca acaccaggtg ctccatttgg tatcttacta aaatcatcaa ttcccaagtc
     9481 tttttgcccc ttaaaattaa atgaacagtg gtctgtgcca actgtttgaa tatctccact
     9541 agacaatcct tcccaaaggt aatttatgtc ttcttttttt cttaaaggag gagacattac
     9601 atacttagca ccctcaaatt tattcttatt atataaattg tcttctaata atagatactg
     9661 tgggcatgtt tctactatca tatctatatt tttttctcta gccattttta cagtttcaag
     9721 tgactcccta caacttaagt ggactacata ggattttgaa ttagataatt tagttatatc
     9781 agcaagtctt gaaacagact ctttctcaac tatgttaggt cttgtaagtg gatgatattt
     9841 tggctctaaa ttaccatttt gtatattttc ttctatcaat acatctagta aatccccatt
     9901 ttcacaatga aatccaacta tacaacctaa atcccttgct ttctttaaaa ccctgtatat
     9961 tgttccatca tctactttca tgccatcata tgccatatac atcttaaaag atactatccc
    10021 atccgaaata agtttttcca tatgattaaa tgtatcttta tttaaacatg taattgtcat
    10081 atgaaaacca taatcacaat aacaatttcc acttgctttt ttatgatatg cttctacacc
    10141 ttgcagtagg ttttccccct cattggcttc tgcaaaatct agtattgtag tagttcctcc
    10201 agctatagct gctcttgttc ctgtttcaaa attatctgca gttgtaatag aacctgcatt
    10261 catatcaaaa tgagtatgtg tgtctattcc accaggaatt acaattttac ctgtagcatc
    10321 aataacctta tcaccattta tttctaagtt atttcctatt tcaactatct tttcatcttc
    10381 tatccttaaa tcacttatat aagtcttatc agaggtaatt atagttccac ctcttagtat
    10441 tgttcccata tatacctcct aacttttaag ctttaaagta ttttctaagt atcgtaaaac
    10501 tttatttaaa attgtatttt ctaaaggtgc tatattcagc acctctagac tatatttaaa
    10561 taatttattg tgaactaaat attattttcc gtatacagat tttaatattc catagtaacc
    10621 ttctgttcct ataaataact gttcttgctc tatatactca tctattgtgt gagcaagatt
    10681 ttccttagaa ggaccaaatc ctatagtttt tattccagct tcacctgcat agtgacttcc
    10741 attcgtacag aatgagtatt gagttatttc tgaatctatt ccagcttctt ttaatccctt
    10801 atatgctgct tgaacaaatt catcttcttc gtcatataac caacctggga agaatctttc
    10861 actttctata gtatttcctg tatagcaatc agctttttca accgcataag aaactttcgc
    10921 atttaattct gggtcttctt tcatcatttc atctaataaa gcttgtattg gagctaatac
    10981 gccttctctt gtttctccaa ctaataatct tctatcaaaa gtagctttgc aataatctgg
    11041 cactactgat gcccctggat atggagaaga ttttatatct gttaaaacta atattccatc
    11101 acctaaaact ggatgagttg gaggtactaa ttgttgtatc ttttggatta cattagccat
    11161 tttataaact gcatttacac ctttttctgg attagcagag tgagctggtt taccaaaagt
    11221 ttctactact atttctcctc tacctctctg accaatcttt aagtttaact ctgaagactc
    11281 acctattaca acatagtcag gttgaacagc cttacttatc tctctagccg aaacaccttc
    11341 aaatatttct tcatgaacaa cacctgctac atatagttct ccagcaaaat ctcgattagt
    11401 gtctttagca aaatacgcta cagcagacat cattgcagta tattgacctt tcatgtcaga
    11461 agttcctcta ccatatatct ttccatcaac tatttttcct tcaaaaggag gaacactcca
    11521 tttacttgca tctggaactg gaactgtatc tatatgtcca tcaaataata ttttcttacc
    11581 tggcttgtta ccttttatac atcctactat atttccatat ctatctcttg accaactatc
    11641 aaaacctaat ttttcaaaag actcttctat tgcttttaca acaccttctt catttccaga
    11701 agaactagga ttttgaatta atttttggca tatttcagtt acttcttgtt ttctttgttc
    11761 gtttaacatt tcattatccc cctagatttt ttataatgta tgtattttat atacttatat
    11821 attttctaaa ttttagaact tatatatctt ctaaatatgt aaaccaatat ctaataattg
    11881 cattttagta attccttata tacttggata ttctccatcc caaactatac ttctatattt
    11941 gtcagggtca gtatctcctt ctgtgcttat taaaagtatt cttgaatttt catctaattt
    12001 taaatctttt cttaaatctg ctaaatcttc tctttccata gctgctacta aaagaccagt
    12061 tgtaactgct ccagattctc cagaaattac cttttcatca ccatttaaag gattacctaa
    12121 tgttctcata cctttagcag aaacccagtc tggtgctgaa acaaatgctg cagcatgatt
    12181 ttttaatact tcaaatccta tagtatttgg ttctccacaa gcaagaccag ccattatagt
    12241 tggcatatct cctccaacaa atcttggttt tccatctcct gcttctgctg atttatagta
    12301 gcaatcagct tcatcagctt ctactactac tgttattggg cattcatcgt atattgaagc
    12361 aacatatcct tgaactgctc cagcaagtga accaacccct gcttgcacaa atacatgtgt
    12421 tggtctatct actccatatt cttttaattg ctctatcgct tcttgagcca tagttccata
    12481 tccttgcatt atccatgctg gaatttcttc atatccatcc catgctgtat cttgaaccat
    12541 tactccatga tcatcagctt ctgcataatc attagctaat cttactgcat catcatagtt
    12601 taagtctgtt atactagcat ctgctccttc tcctttaatt ttatttaatc taaactcaga
    12661 agaacctttt ggcattaata caacagattt ttgacctaat ttattagctg tccaagcaac
    12721 tcctctaccg tgatttccat ctgtagctgt aaagaaagtt atttctccta atttttcttt
    12781 tatttctttc gaagttaatt tgtcataccc taactcagat atatctgtat ctaatctttg
    12841 agctaagtat ctacccatag aataagaacc acctaatact ttaaatgcat ttaatccaaa
    12901 tctataagat tcatctttta catatactcc tgcaacacct aatttttttg ctaaaccttc
    12961 taggtttgct aatggagttt ctttatattg aggaaagctt ttgtgaaaat ttctaacctt
    13021 agttatttct tcttcattta aaaatttgat acaattttcc ttatcaccct ttggcaaatt
    13081 atttactttc catttaattt cttttaacat ctaaaatacc tccaaattat tgttaaattt
    13141 ttatacttta atatataagc aattaacatg ccaacactaa atatatacta gatatttttc
    13201 tatgactctt tagtatagaa attgcaatgc ttttattatt ctttcttttc tatctttagt
    13261 tcatacaatg tatgtagaat ttaataacta aaaaaccttt tctatcttct cactttttaa
    13321 ttctcatttt taaaatttaa aaataaatct caaaacactt tgtttctata ttttttaata
    13381 taaaatttat aaaatctaaa ttaaaacata aaatagtgat gcaataatct attacatcac
    13441 tatttgtata tatttaataa tataataaat ctttagtggt ctctaagagg tgatttttca
    13501 tcttttttaa catctaattc ttcttttacg acattccatt tttcaagacc aaacttagaa
    13561 accataactg cctctgcagc accttttcca agtcttttta ctgtgtccat acctgtctca
    13621 tcctcttggg caccttttgc ttttccatct ttactccata ttgtaccacc tatatagcat
    13681 ccacctaaac ctccagatac taacatttca tgcattagga agaaatttat tataggaagt
    13741 tttgtcaatt cttggccacc atatctagtt ccaccagtag ttatagctgc accaacctta
    13801 ttttgaagtt ttcctggatg aactaaatac attggacgaa gtctattaaa tactgctgta
    13861 agttgagcag ttatattcat atcataaact ggagaaccta ctataattcc atctgcctcc
    13921 aatatttttt gctctaattc ttggatatca tcttttataa tacacatagt ttcttttctt
    13981 atacatgcat cacaatgtac acaaggactt atttttttcc ctctaactga ccaatactca
    14041 gtttctatac caggtatagt ttctgcttct ttcaaagctt ctaacactgc atattctgtt
    14101 gctcctttac gtggacttcc acaaatacct aatatctttg ccataataat atctcctcac
    14161 caaataaatc tatttttatt atctatttac aaatacaata aattgttgtt tgtgctttaa
    14221 tttctactat tttaatttta ttggcaatga atgtatttct acatcagttg cattatatac
    14281 tgcatttgca acagctggcg caactggtac aacagcacat tctccaatac tcttaccacc
    14341 aaaaggtcca gctttttcat aactatttac aaatgcaact tgacattttg gcatatctac
    14401 agttctaaat aatttatatg ttttaaaatc agtagcttta ggtctacctt gttcatcaaa
    14461 tgccaattct tcactcaatg cgtatcctag acccatatga atcccacctt ctaattgacc
    14521 ttctaaattt aaaggattta taactctacc aacatcatgt acagctacaa aatcaactac
    14581 ttttatatcc ttagtttctt tatctactaa aacttcagca aaatgtacac cataagaagt
    14641 aagtccagct ggtgaagaat aatcttcaac aacagttaac tctcttaatg atttactttg
    14701 actatatact gcgacatcag aaagtgatgc ttttacattt tcatcaagtt tactaattac
    14761 atatccattg tctagatata aatcttcttt tgatgtttca agtaattttt ctgcttcttc
    14821 tactataagg tttttcaatt tctctgctgt ctttttagct cctccacctt ctacaaatac
    14881 ccctctactt gcatagtcac caaggttgaa tgtacatgca tccgtatcag tttcaaatgt
    14941 atcaacttta tcaggtgtta ttcctaaaac ttcagctacc atcattgttt gcatagttac
    15001 gacaccatta cccatatcat gtgctcctgt tagtagtgtc aatgtaccat cttcatttaa
    15061 tttaagagtc aatgtaacaa catctctatg tgcaccaaaa actccattac catgtgcacc
    15121 tattgccata cctatacctc ttaaatattt tccttcttct tttggaagtt ttttcttttc
    15181 atcccactta aacatcttct taccttgctc tatacaatcc aatactctag gatttccaag
    15241 agaaccgcgg aagattatat cgacatcatc tggctctact gcatttttat tttgaaaatc
    15301 gactaaatcc atacctattt ccttggctat tttcgcaaat tgagcctgtt gagccataaa
    15361 tatttgaggt gacccatagc ctctcatagc tcctgctatt ggtgtatttg tatatacagg
    15421 catcccagta aactttatat ttggtatttt ataaacttta aatactttat gactaagtgc
    15481 tcctataaca tttaaagcac ttgatgcata tgctcctgta tttgtatata tttttatatc
    15541 ttgtgctatt acttcaccat ctttatttac accagttttt aattttacaa aagacccatg
    15601 acgagttctt gttgatatca tagattcctt tcttgtaaga accattttaa ctggtcttct
    15661 tgtcattttt gataatgcgg ctgctactgg ttcaattgta agttctaatt ttccaccaaa
    15721 tgagcctcca atagcaggtc ttataactct tactttattt aatgacatcc caaaaatttg
    15781 agatagtatt attctaaatc caaatgtatt ttggcaaggt gtatgtacag ttaatttatc
    15841 attataatca tagtgtgcaa ttacactatg agtctctatt gcactatgat gtattgctgg
    15901 agtagagtat tcatcttcaa atatatagtc acattccttg aagccttttt ctacatctcc
    15961 cccatttatg ttgttttgag aaactatatt tgaatctcca tgtatgcaga tagcatcttc
    16021 ttttattgca tcttgtatag taaatacagc tggcaattct tcatattcaa cttttataag
    16081 ttttacagct ttctctgcta tttctggagt ctctgcagct actgctgcaa cacggtcacc
    16141 aacaaatcta actgtatctg aaaatataac ttcattttca ggtatattgt gctcataaaa
    16201 tcttaatgaa ccattgtatt ttctttgtga tgtattgaga taagtagcta tagctctaac
    16261 acctactact ttctcagctt cacttgtatc tatacttttt atttttgcat gtgctacagg
    16321 acttaaaagc atcttagcgt aaagtacatt ttgtggtttc atatcagcca cgtatttcat
    16381 ttgaccagta acttttaaag ctgcatcttt tagagggtat gattttccta ttaccttttc
    16441 cataattatc taccccctaa tctagctgca gataatttta cagcttctat aatctttaca
    16501 tatcctgtgc atctacagag attttcttta aaagcttctt taatttcatc ttcattagga
    16561 gatgcatttc tatttaaaag agcttgtgtt gttataatca taccaggagt acaatatcca
    16621 cattgtacag ctccaacttc tataaaagct tcttgtattg gatgaagatt ttctccatta
    16681 gcaaaacttt ctatagtgta tataacttta ccttcgcagt tttttgcaag aagtttacaa
    16741 gatttcatgt caactccatc tatcaaaact ttacatgctc cacaactgcc actattacaa
    16801 ccttgtttcg ctccagttag atttaatttt tcacgaagta catgcaaaag agtttcattc
    16861 ttttcaactt ctatttcata catgtaatca ttaatattta attttatttt ttccattttc
    16921 tcctcctaca gataaactaa tgttgtaata gaatattatt acaatcttat tttaatatta
    16981 gtataagcta ttccacttat gtccttccat tctattagtt acattagctc tcttacataa
    17041 ttcttctgct gcaagttgtg cattaagcaa cacttgttct tcactttcaa ttgtagttac
    17101 ttttccattt tcaagtatta catttccatc aactaaaaca ctctctacat tgtgcatact
    17161 agctgagtat accaaagttg atacaggatt gtgaagaggt attgattttg gagaaagcat
    17221 tgagttaaat attactaggt cagctttttt cccaatctcc aaagaaccta tttcatcttc
    17281 cattctaaga gttcttgctc cctcaattgt agccatttca agaactttct ctgcagaaat
    17341 tgccagaggg tcacaattat ttactttgtg ttgtaaagct gttaatttca taagctctac
    17401 catatcttgt gcgttattac ttgctgcacc gtcaagacct aaactcacat ttataccttt
    17461 ttttaacatt tctggcacag gtgcaacacc agaagcaaga tacatattac ttgctacatt
    17521 atgagaaact tttatgtcat actttttaag catttccatg tcttcatcag ttatacatac
    17581 acaatgtact gctacaactt ctggacctac tataccccat ttttcaagta attttatatc
    17641 tatttctcca tgtatatcct ttgttgctgt tcttgcaaat tctgtttcag atatatgaac
    17701 tgtaaaatat gaatcatatt ctttaactat attccaaagc atcttaccca tttcttcaga
    17761 tatagcccac attgaagaag gagctacaca gatttttatt cttccatttt ctgaattatg
    17821 atatttttcg aatacccttc tcaaatcttt ttcaaccgtt tccacatctt ctataagctc
    17881 tttgtgaata cctaaatcta tacaacctct acctacaacc cctcttatac ctaaatcttt
    17941 atatgccttt actattccat ctgtaagtcc tggtctattg tgagtatgca tataatcaac
    18001 cattgtagtt ataccacttt ttaaaccttc catacatcca agcattgcag catcataagt
    18061 atcttgttct gttaagaatt ttgctgatgg aaacatcatt gtatttaacc actcatgaag
    18121 tgccatatca tcacctaaac cttttaataa cacttggaac aggtgattat gagtatttat
    18181 aaagccaggg aatattactt ttccattagc atctataact tttttagcat caagatactt
    18241 aggctcaata tcaactgtat ttcctatatc caagattctg tcaccttgaa tagctattgc
    18301 tccatcaaat ataacatctc tgtctttatt aactgtaaca attattgcat tttttattag
    18361 tatatcaatc atcttaaatc cccctttgaa tactttcatt acaacatttt accatatggt
    18421 tttctcatat tatatctaat acatttatcc aatattttcc tcaagatttg aagtaacgtt
    18481 ttcagcactt ttacgtgact ttaatttaaa taatgtttga agtaatatag ctattattgc
    18541 tccagaaact atacttgacc ctccaaggac agttccaatt gtttctggga attggtctaa
    18601 aacttgtgga gcagttgtaa gacccatact tgttgctata gatacacctg ctataagatt
    18661 gttttcttca gtgaatccat ccatactcat aatagagaaa ccagacattg ctattgatga
    18721 gaaaactact aaagttgctc caccaacaac acaagatggt atagttgtca ttattgcacc
    18781 caatgctgga gatatccctg ctagtaataa tacaagtgcc cctagtgcta ttacaaatct
    18841 acttgtaact ttgttcatag atacaattac tgtattttgg ctaaatactc cagttggaag
    18901 agctgcaaat agtgatgaaa ttatagaagt tatagaattt cctaaaacaa ctgatgctaa
    18961 ttcttcgtca gttacctctc tatccattgc tcctactgta gctattgtac aggcacccat
    19021 catatcagct atacctatta tgtaaataat agtaaacatt actattaact ctggtctaaa
    19081 ttctaatcca aaagctgctg gtgttggtaa agatactata gctgctcctt gaacggctga
    19141 gaaatcaacc aatcctaaaa ctaaagatat agcataacct actaatattc ctattaatat
    19201 tgaagaattt tttactaaac ctttaccgta tttatttatc attattatta caaaagcaac
    19261 tataactcct actgtgaagt ttattgggct tccaaatgtt tgtgtgcctt ctcctccagc
    19321 taaattcttt atagctgttg cgaatagtcc aagtcccata catgctatta tagttccaga
    19381 aactattgga gtgaatatat gtctaatttt cttaactcca aatcctacaa agaatacgat
    19441 taccccagca gcaatctgtg ctccaaatag agctgctaaa ccatctttag ctgctactga
    19501 aagacaagct cctaaaaata cataactcat tcccatcatt acaggtaatc ttgaacctaa
    19561 cttaaatcct ttaaataaag gtattggata taactgtaaa aacgttgcta aacctgctgc
    19621 taacatagaa ccctgaatca acattgttgc ttgcccctgg ttaagtccta ccacgtttgc
    19681 tattaatatc gcaggtacca tatttcctac tatcattgct aacagatgtt gtaaggccaa
    19741 aggtattgat tcctttagtg gtggtatccc atcgacatca tattttgaag tattacgtgt
    19801 tttcataaaa taaatcctcc catacataaa tatcttgact aaatattttt ataattattt
    19861 tctataaata tttttatgac catgaattta ttgctcaacc gataaacaca gctaaactga
    19921 gctattttta taaaaaatta acaaatttct aaatattccc tccccgattt tcaacttaaa
    19981 tgaaacatta attgcctggt atatatttat ttgttaaatt ttttattaaa atgaatagct
    20041 atcaaaaata atctaacaat ctcatactaa ataatttaaa caattctttt aaattattat
    20101 atcttaatta gtctcttcca taacagctct ttctttagcc tcatcctcta gacttttatt
    20161 tggtattatt agatttaaaa cgaatgcaac caatgttgca attactattg gagattctcc
    20221 aaatactaac tttacaaatg aaggaaatgc ctcttgggct gctggtactg atgttattcc
    20281 catgcctaag gcaattgcca aaccaacaat ggtaatattt cttgatgaca actcttctgt
    20341 aataattaac tttatacctg tcattgttat catactaaat atagatattg tagctcctcc
    20401 taaaacagaa tatggtatag tagtcattaa tgcaccaaat ttaggcacaa accccgacaa
    20461 taacataaat ataccagcta ttccaagcac aaatctactt attactttgt tcatagctac
    20521 catccccacg ttttggctat atgatgatgg tggtaaacct ccaaacaaag ctcctatcat
    20581 agtacaaatt ccacttccta ttactccact tgataattcc ttatctgtga tattcctatc
    20641 catgcctccc attgtagtag ctgataaatc tccaattgct tgaacagcat tgattatact
    20701 tacaattact actgagatta ttacaggcat atcaaattcc atcccaaatt caagcggttt
    20761 aggtatcgca aaccaagttg cctctctaat agggtcaaaa tttattatac ccagaactaa
    20821 agatacacaa tatccaacta taattcccaa aaatattgct gccaattttg catatccttt
    20881 tgcaaattga ctaagaccta taactaccac taatgttata gccgctactg cccagttttg
    20941 catagaggca taagtaggac tatttacacc acctgccata taatttattg ctataggata
    21001 caaagataat ccaattgtaa aaaccactgt tcctgcaact atattcggaa aaaattttct
    21061 tatctttttt atgaatatcc ctattaatac tgtagccact ccccctatta attgtgcacc
    21121 caaaatgctt gctaatccat aatttcctcc gactgctaca agtgttggta cataagtaaa
    21181 accaactcca aaaattatag gcaatcttga accaatattt cctattggat atagttgtat
    21241 aagtgttgat ataccagcta ttattaatgc atattgcact aaaagtgtta tttgtgtagg
    21301 gctaacaccc acagctcctc ctacgactat aggtactgta acagttccaa ctatcattgc
    21361 tagtatatgt tgcattgata gtggcaaaca ctttaaaatt ggtggccttc catcaatatc
    21421 aaacatagaa acattctttt gatatttact caaggttatc actcccaact attttttaat
    21481 gacagttatt attatctcac acaacatcct ttcttttttt attttgtcat ctcaatttaa
    21541 tatgaagcaa ttataatgcc aattttttaa cataattgtt ttacatatat aatttttatt
    21601 atatttgtta gtatttgata atttattaaa gcatttattt ttttaaattc ttttaaattt
    21661 tttttatttt tggataataa aatgctatta cacaaaaaaa cacttagaaa atagttttcc
    21721 aagtgttttt tatcaatttt ataatttcaa attttagaat tgagaataaa aaacctaatt
    21781 attatacatc tatttttctg tacaatgtag ctagtgatat tccaagtttt ttggcagcca
    21841 gttttttaga cttagtatca tttccgtaaa actttaacac tttctttata taggctcttt
    21901 caaactcttc aagtgtaagt atgtcttgtt ccatacttat ctcatagtca ctttcctcaa
    21961 gtaactttat atttatatta tttaacttaa aatattcaag tatctttcta tttattattc
    22021 ctttatgtat tattccatct tcctcaagaa ggtttaatgc cagttctata gtattttcca
    22081 actctctaat atttcctggc caaggatact taagcaacat ttgttccaca tctggttcta
    22141 tatggtacac gtttttcttc atttttctcg aatatttgtc tataaaatta tttattaata
    22201 tttgtatatc gtctcctctt tctctaagtg gaggcacttc taaaggtatt acattcaatc
    22261 tatagtataa gtcttctcta aatttatttt ccttaaccaa ctcaaataaa tctctattag
    22321 tagctgatat gattctaaca tctatatcaa ttagttcatt tgaacctatt ctcgtaaact
    22381 tcttttcctg taataccctc aataactttg attgtagttg aattggcata tctcctatct
    22441 catctaaaaa tacaacccct ccattggcca attcaaactt accaatttta ccttttgaat
    22501 ctgctccact aaaagctcct tttgtatatc caaaaagttc actttctagt aaaggttctg
    22561 gtatagcagc acagtttata gcaacgaatg ttttattccc tctgtttcct gcgagatgaa
    22621 tggctcttgc tattaattct tttccagtgc cactttctcc agttatagct acagtagaat
    22681 cactatttcc aattttgcaa attctattct taagagcacg cattacatca gactctccaa
    22741 ttatggagtc tactgttgtt gttccccaaa tattatatac ttccattcca ccatacatta
    22801 ctgaatcact gtgtttgtcc ataaaatatc aactccaaat aagtattcta tataataatc
    22861 attcacatta tctatttaat tatatcataa cagaaaagct tagttataat ttaataatat
    22921 tattttaaaa caaaacttaa taaaaagtat atctacttag ttttaatttg atagat
//
LOCUS       DAELXX010000064        22770 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.064, whole genome shotgun sequence.
ACCESSION   DAELXX010000064 DAELXX010000000
VERSION     DAELXX010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22770
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.064"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            41..877
                     /locus_tag="KPH29_002271"
     CDS             41..877
                     /locus_tag="KPH29_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG1467860.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFKFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1230..2807
                     /locus_tag="KPH29_002272"
     CDS             1230..2807
                     /locus_tag="KPH29_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG1467861.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGLANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSDFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            2800..3984
                     /locus_tag="KPH29_002273"
     CDS             2800..3984
                     /locus_tag="KPH29_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG1467862.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVITNNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPTGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGEEGEFYLRLNVACSREKLEDGLNRIKKTMDYIAKR
                     TL"
     gene            4002..4601
                     /locus_tag="KPH29_002274"
     CDS             4002..4601
                     /locus_tag="KPH29_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG1467863.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4625..5095
                     /locus_tag="KPH29_002275"
     CDS             4625..5095
                     /locus_tag="KPH29_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG1467864.1"
                     /translation="MFKMFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEALVSMGEQVK
                     EGTPMLKINRPLIELKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
     gene            5452..6198
                     /locus_tag="KPH29_002276"
     CDS             5452..6198
                     /locus_tag="KPH29_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBG1467865.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHMELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKLYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            6260..6934
                     /locus_tag="KPH29_002277"
     CDS             6260..6934
                     /locus_tag="KPH29_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG1467866.1"
                     /translation="MNDELKSILYNKGVDIVRFVDISEFPINQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKVEELADWISKYIQNKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCLNCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCALRCMMSCPWSL
                     RYANQK"
     gene            7302..8648
                     /locus_tag="KPH29_002278"
     CDS             7302..8648
                     /locus_tag="KPH29_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBG1467867.1"
                     /translation="MEIETKTPDWINVLELNSKIDITWKYDVSNMIQDIVSDKSLEGS
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGGDNQVSNLDKKQSIVGTTNFITRGVSNI
                     SIITLEGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLFHFEHLFISGFSGI
                     GIEIPYYSTGNDIIVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     REIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMCYSQITARITKCGQYYKSVDYNTYE
                     NMEDRKEEAYSIFYGDILENCNIVLMNSNIDTLENDSLDEHKIYVIKANETNKVMLTC
                     YAEADELIQCAKGNLLLENGENTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNTVIYSVLDSIEEINKLHGGIWEQIDVKVLAGESMYYYKKIKD"
     gene            8675..10075
                     /locus_tag="KPH29_002279"
     CDS             8675..10075
                     /locus_tag="KPH29_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBG1467868.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSSKLQEIIDAAPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENIAI
                     ITAKSVRHCIKSICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAIVCEDKQ
                     GEPGHYENLFFSGFSGIALDMPNNSTANDITVFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNKDNWEDFKEDKH
                     KVYLVEALETKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSSINGIGIS
                     DEDYGDKVKIKKGNIYINSKDKVIKMHKPEVGDIMSTIIKDENILSAHYGGTWGKIGE
                     KIEFGITVHYYKLIEK"
     gene            10285..11721
                     /locus_tag="KPH29_002280"
     CDS             10285..11721
                     /locus_tag="KPH29_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBG1467869.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNIGPDLQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSPTETTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNEEPPTKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKNMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKDYEGYGSYKINNITIDQCGYCGFLFDSVKGSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSSPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIETSYGGKW
                     EKLGSEVIGKETVYYYKHHEPDKTNQIK"
     gene            12065..13009
                     /locus_tag="KPH29_002281"
     CDS             12065..13009
                     /locus_tag="KPH29_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HBG1467870.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLKEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLKDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGIILEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEDNKLLIDTLRDDAKISIRDMKTIKVDKNKQISIFD"
     gene            13178..14110
                     /locus_tag="KPH29_002282"
     CDS             13178..14110
                     /locus_tag="KPH29_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBG1467871.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENPLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKAK"
     gene            14215..14682
                     /locus_tag="KPH29_002283"
     CDS             14215..14682
                     /locus_tag="KPH29_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C_GCAxxG_C_C family protein"
                     /protein_id="HBG1467872.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGETMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(14926..15780)
                     /locus_tag="KPH29_002284"
     CDS             complement(14926..15780)
                     /locus_tag="KPH29_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HBG1467873.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYRENVLFNLITGILWSI
                     ANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGIILIMSG
                     VFMLSIIK"
     gene            15946..17910
                     /locus_tag="KPH29_002285"
     CDS             15946..17910
                     /locus_tag="KPH29_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG1467874.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLSEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYIGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVAADQHRDTNELKNTSTNPCETSTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSCCTSKK
                     YKSFRNLIFEENILLNIEASNYEDAIKYITDNLIKKGFVKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLLEESI"
     gene            17910..18371
                     /locus_tag="KPH29_002286"
     CDS             17910..18371
                     /locus_tag="KPH29_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG1467875.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSDAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            18374..18694
                     /locus_tag="KPH29_002287"
     CDS             18374..18694
                     /locus_tag="KPH29_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBG1467876.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            18710..19810
                     /locus_tag="KPH29_002288"
     CDS             18710..19810
                     /locus_tag="KPH29_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBG1467877.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            19930..22530
                     /locus_tag="KPH29_002289"
     CDS             19930..22530
                     /locus_tag="KPH29_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBG1467878.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKHVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDYILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNVFIEESGYYILKVR
                     INVQLPALGYKVVSFESSDKEMVSLDKSNDTFISNDNYKIIYEDGSLNLQLKDGTYIS
                     NFLILKDSGNAGDTYDFSPLKNDEDIKLSFDRVYTEKTLGYEKLVVKGTTLLPLTLED
                     RKNKYLNGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRENGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTVFTRGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKKLEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYRIDNKIQKSIVNKTVSRELEIISLPEKY
                     LISACYPSYYSKDKFIIRVENPSSNRMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
BASE COUNT         8940 a         2350 c         4026 g         7454 t
ORIGIN      
        1 tgtaagtgtg atgagagaat ttttttagag gggatttata gtgaatataa aaaagatatt
       61 taataacaat gtagtagtaa gctccctaga agatggcaca gaaataattg tcactggtgc
      121 tggagtagga tttaagaaaa aagtaggcga tttgatagat gaaaatttga tttctaagaa
      181 atattttgtt caagacgatc aaagggacaa atacaatcaa atacttaata agacctctat
      241 tgaatacttt aaaatttcag aagaaattat agaaaaggct aatgaagttt taaatactca
      301 agtgaatgat tcaataatat tagccttaac tagtcacata gaatttgctg tccaaagaga
      361 aaaacaaggt ataaaattac caaacttaat attgaatgag acaaaacaac tttataggga
      421 agaatttaaa tttggattat gggcaattga tgaaattgaa aaaaggatag gaataaaatt
      481 gcctgaagat gaagcaggat atattgctat tcacataatc aatggtttgg aaaattctca
      541 aaaagatgaa ggtattaata tactagagtt ttcaaaacaa gttattcaga taattgaaga
      601 agtacatgga tttaaattgg atgtaaattc attaaattat actagactta taactcactt
      661 aaagtttttt gttcaaagga ttctccgaaa agaaacttac aatgatagtg atattgaaga
      721 tatgtataag ttagtgaata aaaattacaa taaacctaaa gtgtgtactg aagaaattgc
      781 agcattggta ttagacagat ttgaatataa gattagtaaa gaagaagaat tatatttaat
      841 gattcacata aataaaataa caaacagtgg ttgttaggat tgtaactgat aaagcaggca
      901 aaacctaagt caatgagctt aactattaga ttaagttttt tgatttaggt ttttttatta
      961 gatacaatca aaatacagac agtgactttt aggattgtaa ctggttaagc aggcaaaacc
     1021 taaatcattg aatttaacta ttaggttaag ttttttgatt taggtttttt tattagatac
     1081 aatcaaatac agacagtgac ttttaggatt gtaactggtt gagcaggcaa aacctaaatc
     1141 attaaactta attattaaat taagtttttt gatttaggtt tttctataaa tttgattatg
     1201 tttttagata aaaataggag gggaaaatta tgaaaaaaaa taagttaggt ttatggaact
     1261 tcttccaaat gttagggaaa acgtttatgt tcccaatagc actacttagt gtttctggga
     1321 tgatgctagg acttggggca ggttttacag accctaagat gattgaaatg gttccatttc
     1381 tagggaatga atatgttaac ttagttttaa acttcatgtt aacaatagga ttatttgcat
     1441 ttaacaactt gggagcatta tttgcaatgg caattccact aggattatta aaaaaagaaa
     1501 aagagtttgg tgctttttca ggattagtag gatttatagc aatgcatata ggtacaaatt
     1561 tttatttaac aagagctgat ttgttagtac cagcagaaca gatgtcaact aatggtcaag
     1621 ctatgattat gggtatacaa acatataata caagtgtttt aggtggtatt gtagcaggat
     1681 taattgttta tgcaatatat gataaagtta gtaatttaaa gataccagaa tcattaggat
     1741 tctacagtgg accaagatta gttccaattg ttagtttaat agttatgagt atagttggat
     1801 tattaatacc tattgtttgg ccaccatttt tcaatgcatt ccaaagttta ggaagatgga
     1861 tttcttcagc aggaccatta ggatattttt catatgcagt ggcagaaaga gttacaattc
     1921 catttggact aaatcatctt gtaacatctg tattccgttt tacaccaata ggtggaactg
     1981 ctgttattga tggagaaacg tactttggta cattgaacat gtttatggca tatgtagaaa
     2041 ataatcagat tatacctctt gatttagctg gtaaaatgga acaaggtaaa ttgatgattc
     2101 aatatggatt agctggtgct gcattggcta tctaccgtac agcaaaacca gaaaatagaa
     2161 aggcacttaa aggactttta atatcaggag tgttgacagt tattataggt ggtataagtg
     2221 aaccaataga gtttctattc ttattcatat caccagcatt atttgctttc catacattta
     2281 tgaatggtct agcaaacatg gtacttccat acttaggcgt gttgatggga ttcacaggag
     2341 atttaattgc atttataagt tttggtgtgt taagaggaac agctacagga tggccaatag
     2401 cagtattagt agcagtttta tattttgcaa tatactattt tgtatttaag tgggcaatta
     2461 ttaagtttaa tataaagacc ccaggacgtg aggataaaga aataaaagta tctgaaggaa
     2521 atggttcaga tttcaaaaat ttgtctacat ataaaggaca acaaatgata tctgctttag
     2581 gtggtcaatc taatatatta tcactagata attgtgtaac tagattgcgt ttaaaactac
     2641 aagatgtatc attaataaat gaggatgcaa ttaaagaggc tggtggtata gcagttgtta
     2701 aattggatga gcatacaata caagtaatta taggaactca agtgtattct ttaagaaagc
     2761 agatggataa agctatggaa ggtcatttgg aggcatgtaa tgattaattt tgataaaaaa
     2821 gtagaccgat tgggtacata ttgtacccag tgggactatg tagaagatag atttggtaag
     2881 aaggatttgc ttccttttac aatttcagat atggatttag agtttccatt tgaaataaca
     2941 gaggtgttgc aggaaaggtt aaatcataga gttttgggat acagtagatg gaaacatgat
     3001 gattttagaa attcagttac gaattggtat attaaaagat ttaattgtag cgttgatagt
     3061 aactggatat attatagtcc aagtgtaatg tattcaatta gcaaacttat tgagatgtta
     3121 agtaatgaag gagatggaat ccttataaat actcctgcat acaatgcttt ttatgatgtg
     3181 ataacaaata acaatcgtaa tattataaag tcacatcttg taaataggaa tggattctat
     3241 tcaattgatt ttaatgattt tgagaaaaag tgtaaaaaat ctaagatatt tattttatgt
     3301 aatcctcaca atccaactgg tagagtatgg agtgagttag aacttgttaa gatgatagga
     3361 atttgtaaga ataatgatgt caagataata tcagatgata tacacatgga tattgtatat
     3421 gacaatagaa tgacacctat cttaaatgtt gcaaaggact atttagattc tgtattcatt
     3481 tgtacatcag cgtctaaatc tttcaatata ccagcattga ctgggtcata tgtgattata
     3541 ccaaatgaag gtattagaaa taaatttgaa aatatcacta gataccgtga ttttgtaaat
     3601 tcgccagcaa ttctagcaat attagctaca atggtctgtt atgatgactg cgaatattgg
     3661 ttagatgaac tattaaaata ccttaaagag aatcttaaat atacgattga ttatataaaa
     3721 gataatttag aaccattgaa attggtcatg ccagaaggct gttactttgc ttggatagat
     3781 ttttccgaac ttaaaataag tggtgagaaa ttccaaaaat tattgatgga tattggagaa
     3841 gttgctatta tgtcggggtc tgtatatggt gaagaaggtg agttttattt aaggttaaat
     3901 gtggcatgtt caagagaaaa acttgaagat ggtttaaata ggattaaaaa aactatggat
     3961 tatattgcaa aaagaacttt atagtaggaa gggagatagt tatgggatat ttaaaaacaa
     4021 tggttgatgt tatgactact gaaatgaatg ccataagatg tttaatagat gaagcaggaa
     4081 tagaatatga aagtatagtt agtgaaattg caaattgtga aggcaaagta atttttatgg
     4141 gtgtaggaaa atctgcacat ataggtaaga aattggcagc tacatttgct agtactggta
     4201 cacctagctt ttttgtacat gcaacagagg cagtgcatgg agatttagga atgattgaaa
     4261 gtaaggatat tacaatttta atatcaaata gtggaaattc tatggaagtg gtaaactgta
     4321 taaaatatat taaagcaata ggttctaaga caattgcatt tacttcaaac agaaattcag
     4381 tactggcaaa agagtgtgat tatgctttaa tatatcctgc aaaggatgaa gcagaccatt
     4441 taaatctagc accaacaaca tcatctacaa ttactttggt tttaggagat tctattgcat
     4501 gtgcattatc taagagtagt aattttggtt cttcagattt ttataagtac catccaggag
     4561 gaagtttagg tgagaagtta aaaacagcta acaatggata aaagcaaaaa ggagagtagt
     4621 ttttatgttc aaaatgttta aaaaaaaatc aataaaatca cccatatcag gaaaagtaat
     4681 agagttatct aaaatacaag atggtgtatt ttctcaaaaa attatgggag agggtgtagc
     4741 tatagattct acaggagaca ttgtatatgc tccaatcaat ggaaagatag tagtagtagc
     4801 agagactaaa catgcatttg taatagaatt agaaaatggg atggaacttc ttattcatgt
     4861 tggtttagat actgtaaatt taaaaggcga aggttttgag gcattggtga gtatgggaga
     4921 acaagtgaag gaaggaacac caatgttgaa aattaaccgt cctttgattg aattaaaggg
     4981 tatatcactt attactccag ttacaataac taatcattca gagtacaata tgaatacatg
     5041 taatgtaggc aatagcgttg aaggtgggaa agatactgta attgagttta aataaagaat
     5101 taaaatacaa ggttaatatt tataaatacc tataaactta tgttttataa gattgattgt
     5161 atggatttat tgtataaaaa agaactcttt aattataaat taaggagttt ttttattttg
     5221 taaatattgt ttagatttat atagaaaaac acttagtaat aaatagaata aataagtatt
     5281 tatatagcta aaaatctaaa tagttttgtt taaatatatt tttatatttt gtaatttcaa
     5341 attaataaat aattagaatt gataaacaaa aaataaaaat gttgattata ttaataaact
     5401 ttaatataat atatttatga aaaatgtaaa ttgtgaaatg gaggtagtaa catgaaaatt
     5461 gaaaatcttt ctattgatga tattaaacga ggctatatat ttaatgatga tttaaattgt
     5521 tatagttgca ttaaatgtac aaaaacatat gaggtaggag aaatatataa aataggtaat
     5581 agattctttg aagcttcaaa agctattgaa cttcatatgg aattagaaca tagtgattat
     5641 ttagagcaat tgcttaatag tgattcaaag tacaacacat tgactgaaaa tcaaaaacaa
     5701 ttatttcaat tatttattag tgatttacca gataaggaga tagcaaaaca ggttggagta
     5761 tccacatcta ctatacgcca tcaaaaattt attttcagag aaaaagcaaa acaggctaag
     5821 ctctatttag cactatatga atctgtattt gaagataaat ctaataaaag tagtactata
     5881 ataccaatcc atgaacatgc tcgaatggta gatgaaagat atgtagtaac tgaagaagag
     5941 agaaaacaca ttttggatac atcatttgaa agtatgaatc ctttaaaatt aaaagtattt
     6001 tcttcaaaag aaaagaaaaa ggtcgtaatt ttatctaaga ttttggaaca atttgaacgt
     6061 gggaagaaat attcagaaaa ggaagttaat cagattttag aatctatata tgatgatttt
     6121 gcaacaatta gaagatattt aattgagtat ggctttatga gtagaaataa agaatgtaca
     6181 aagtattggt taacataatt aaaataataa attttactaa tgtgtaatgt tatttttatc
     6241 taggggggag gagttaaata tgaatgatga attaaaatct attttataca ataaaggtgt
     6301 agacattgtt cgctttgttg atatatctga atttccaata aatcaaacac aaggtttttc
     6361 taaagcaatt ttattttgta tagggttatc aaaaaaattt attacagata tatacaataa
     6421 cttacctaca gatagtgatg agtttttgga aaaagaggag aaagtagaag aattagctga
     6481 ctggatttca aaatatattc aaaataaagg atatagagcg tattctcaat cggagaaaaa
     6541 caatctagaa catggatatt ttgaaaaagc ctatattaat ccagaaatgc aatcagggat
     6601 tagtccttta ccacataaga caatagcaaa tattagtggc attggattta tgggaaaaaa
     6661 taatttgttt gtaacagagg aatacggatg tgcttttagc atgtgtacag tacttacaga
     6721 tgctccaatt tctgttgaaa gatatccttt gattgattca aaatgtttaa attgtaatgt
     6781 ttgtgtagag aactgtcctg caaaagctat acatggaaat gaatggactt tacctggaaa
     6841 aagagaatct attattgatg tttcaaagtg tttttgtgca ttgaggtgta tgatgagttg
     6901 cccatggtca ttaagatatg caaatcaaaa ataactttac tagagaaggt attaaattga
     6961 atattataaa atttttaaaa tgtaagatta tataagattt ctcaaaataa aaatttgtat
     7021 agaacactca gaaatgagtg tttttctatt gggattaaat tatattgata caaagcttaa
     7081 ctattttaat cattatataa aatttgtatt aaaaaattaa gaataacttg ctaatagtaa
     7141 aaatataatg agattataat tgatttataa tataactgta atgatgttgt aaaatgaaaa
     7201 ttgtcatctc aaaattccca aaaagagtaa aagtcacttc ggttatagtc aaaaaatttt
     7261 ttctgtgaaa agaatttaga tttaaaaggg aggaattaat catggaaatt gaaactaaaa
     7321 ctccagattg gattaatgta ctagaattaa acagtaaaat agatattact tggaaatatg
     7381 atgtaagtaa tatgatacaa gatattgtct ctgataagtc actagaaggt tccattatat
     7441 attttccaaa aggtaattac ttatttgaga aaggtattaa aattagtcaa caaataacac
     7501 ttcaaggaga ttcttattat ggaggagata atcaagtatc aaatttagat aaaaaacaat
     7561 caatcgtagg aacaaccaat tttataaccc gaggagtttc taatataagt attattacat
     7621 tggaaggaac aagtcaatgt atcaaaaata ttaattttta ttatgatagt catgatatag
     7681 agaaaatacc acctaaaaat gtatctgcta ttacagaata tggagaaaca caaggactat
     7741 ttcactttga acacctgttt atttctggat tttcaggaat agggattgaa attccatact
     7801 attcaactgg caatgatatc attgttagtt catgtggtct tggtatgagg cttggagaga
     7861 agtcaatgct ttctagtagc aagatttatg aatgtaaaaa tggaatggag attactactg
     7921 gtgttagctt aaataatgtg agaatagaag aaataagaga aataggtatt aataataaag
     7981 gatttggttt taacctgatt atgaatctta tggtcgacca atgtggatat tgtggattta
     8041 tgtttgaaaa aatgtgttat agccagataa ctgccagaat tactaaatgc ggtcagtatt
     8101 ataaaagtgt tgattataat acttatgaga atatggaaga tagaaaagaa gaagcttatt
     8161 ccatttttta tggagatatt ttagaaaatt gcaatattgt attgatgaat agtaatattg
     8221 ataccttaga aaatgattca cttgatgaac ataagatata tgtaatcaag gcaaatgaaa
     8281 caaacaaagt aatgttaaca tgttatgctg aagcagatga gcttatacag tgcgcaaagg
     8341 gcaacttgtt gctagaaaat ggtgaaaata cttataaatt ttataatggt gagatttgtt
     8401 ctgtaggagg tgttcaaatt tctgatatag atgaaaaaga tttaatcaaa ataatcgaga
     8461 acactctgta tatagatgat aaaggtaagt acttaatggt tcctagatta aaaaagaata
     8521 ctgtaatata ttcagtacta gattctatag aagaaataaa taaattacat ggtggaatat
     8581 gggaacagat tgatgtaaaa gtattagctg gggaatctat gtactattat aaaaagataa
     8641 aagattaaat tatcaaacta ggaggtagtg attaatggaa gagtctataa taattaaaga
     8701 tataagtggc tggattaatg tacttgatta tgggatttgt aatgatgaaa caaaagatgt
     8761 aagttccaag ttgcaagaga ttatagatgc tgcaccagaa gattctatta tatattttcc
     8821 aaaaggaaaa tacttatttg aaagtggtgt taagattaag aaacgattaa ccttatgtgg
     8881 agattcttat attttggcga atgttcctaa aaacactgct ggagtaacac aatttacttt
     8941 taaaggaaag aaaaatccta aaagtgaaaa tatagcaatt ataacagcaa aaagtgtaag
     9001 acactgtatt aagagcattt gcttttattc agatagttgt gatagagata tgacatctaa
     9061 tcatgaacct ccaacagatg gaaaacctag atttcatcat aatgtaatta taaattatag
     9121 aaatatatcg gctattgtat gtgaagataa acagggagaa ccaggtcatt atgaaaactt
     9181 gttttttagt ggtttttctg gaattgcact tgatatgcct aataattcta cagcaaatga
     9241 tattacagtt tttacatccg gacttggaat tagtacaggg gaaaattcaa cgattgctaa
     9301 cagtaaggtt tggggctgtg ttgatggaat gataatttct actggaactt ttataaatgg
     9361 tatgagggta gaagaaataa aaaaagttgg aattaaaaat attggtagag gtttaaattt
     9421 tattacaaat atcacaattg accaatgtgg atattgtgga gtttggtttg attctatatc
     9481 caatgttcag atatcaggaa atatcacaag gtgcggacag tactattatg atgtaagcta
     9541 tgataattat ttaatgttgg ataatagagt taaagaagca tattctttat tctatggtaa
     9601 tttattggag tcaagtaata tagttttgtc aaataccaat aaagataatt gggaagattt
     9661 caaagaggat aagcataagg tgtatcttgt agaagcactt gaaaccaaga atgtactatt
     9721 aacatgcaat atagatgcta ctgactggat tgttaagtca gaaaaaggaa atttaacgta
     9781 caataatatg cgacaaactg ttatatttta cgatggaaaa ctaagtagta tcaatggaat
     9841 tggtatatct gatgaagatt atggggataa agtcaagata aaaaaaggaa atatatatat
     9901 aaatagtaag gataaggtta ttaagatgca taaaccagag gtaggtgaca ttatgtcaac
     9961 aattattaaa gatgaaaata tactttccgc tcattatggg ggaacatggg ggaaaattgg
    10021 tgaaaaaata gagtttggaa taactgtaca ctattataaa ttgatagaga aataagtaaa
    10081 tacgtagtat ttgaatagat gtattttatt ggagagctag gactatagtt ttaatgtaaa
    10141 tggtttggta atagcatgga ttgaataata taaaaataat ataaaaaatt aatttgatta
    10201 tgtaaaattc tatgaagtag taaacctgtg actttagtca tggtaggttc aaattaagta
    10261 aatgggaaag gaatatataa tattatggaa ggaagaactg ggattaaaga tggaagatgg
    10321 attaatgtag tagaaagagg tattaaaaat gatggttcgt acaacatagg tcctgattta
    10381 caggatatta ttgatgaaat agaaacagat ttctgtgtct tatattttcc aaaaggaaaa
    10441 tacttattta caagtggtgt taaaattaat aaagaaattg tattacaagg tgattctaat
    10501 tcacctacag agactacaca atttataact cgtggagtag aaaatatgag tattattaca
    10561 ttgacaggga agaaacaatg tattaaaaat ataaatttct attctgatag ttgtgagata
    10621 caggtaaatg aagaaccacc gacaaaaggt aatcctaaat atcattatga gatgattatt
    10681 aataagaatg aaaaaaatgt tgagatgaat aatgtatcag caattctgta tgataatagt
    10741 aagaatatga ttaaaggtct aggtcattat gaaaatattt atataagtgg tttttcgggt
    10801 acaggaatta ggataccata ttactcaatt gtaaatgata ttacagtttc atcttgtggg
    10861 ttgggtattg atacaggaat agacacaatt atttctaata gtagaatatc taagtgtcaa
    10921 aatggaatga ggattagaac tggaactagc ataaataatg taagaattga aaaaatacag
    10981 aaagttggtc ttataagtag tcaagttaag gattatgagg gatatggaag ttataaaatt
    11041 aataatatta cgattgacca atgtgggtat tgtggttttt tatttgactc agtgaaaggt
    11101 agttattttt ctggtattat aagaagatgt gggcagtatt attataatac agattatgac
    11161 acatatttaa gtattaaaga tagagtagaa gaagcatatt ctttatttta tggaaattat
    11221 tttacagaat gtaatattga tttagttagt gataataagg ataactggga tgatggctta
    11281 gaatataaac ataaagttta tgtatttaaa acccttcaaa taccaaaatt aacattgaga
    11341 tgcaatatag atgcctatga ttatatcgtt aaatcggtac atggaggagg taatttaatg
    11401 ttaaaaaata actttaacaa ttatatattt attgattcat ctagtccaga agttattaat
    11461 ggaattaatt ttaattatca aaagaatgga aacctgttaa ggttaaataa tggtaaatta
    11521 agttataaaa atggaaagga agaaacagct atattcagac ctaattatgg tcttattgta
    11581 gcatcaacag attcagaatg tgaacaaata gagactagct atggaggaaa atgggagaag
    11641 cttggttctg aagtaattgg aaaagaaacg gtatattatt ataaacatca tgaaccagat
    11701 aaaacaaacc aaataaaata aaatttgtat tatataatga tataaagttt taatatatag
    11761 tgtttacctt taatgtaata ttggtatatt tgtatagtat taaatttgtt aaaaagtaaa
    11821 attgtagggt gcttgaaaat agcacctttt ttagtttcat tataaaatta gttttgaaag
    11881 tttaggaata atgtatattt atttttaaag aattataacc ttattaagga tattttaaat
    11941 gctagaaatg aatcagtaaa tcatactgga attatcttaa atgttttgat taaattaagt
    12001 atttagagct ataattatat taaaaatata ttaggacata attatattac tatggaggtt
    12061 aaatatgata gtaagtgtga gtagaagaac tgatatacct gctttctata gtaagtggtt
    12121 ttttaacaga cttaaagaag gttttgttta tgtaattaat ccaatgaatc caaaacaggt
    12181 tagtaagata gagttaaatc cacatactgt tgattgtttt gtattttgga caaaggatgt
    12241 aactccaatg ttatatgatt taaataaatt aaaagactat aaatattatt ttcactatac
    12301 tataacatca tatggaaaag aagtagagac atgtatttta gataaaagaa aggtaataga
    12361 ttcatttaag gaattgtcta gaaaaatagg caaagaaaga gtaattttaa gatacgaccc
    12421 gattttctta agtgaaaagt acacagtaga ttatcatatc aaagcatttg cgagtttatg
    12481 taatcagctt gatggattta cagaaaaatg tataataagt tttatcgatt tatataagaa
    12541 aacaaaattt aatactaaag cattacatat aaagccaata gaaattacag aaatagaaac
    12601 attatcaaaa gaaatttcta agattgctca taagcatggg ataatacttg aaatgtgttc
    12661 tgaagaatat aatttaagta aatttgggat tggaaaaagt aagtgtattg atgatagatt
    12721 aatatcaaaa attataggat gtgaagtaga ggtaaaaaaa gatagtacac aaagagatat
    12781 ttgtggatgt gttaaaagtg tcgatatagg acaatacaat acttgcagac attattgtct
    12841 gtattgttat gctaacttta attatgcaaa agtagaagaa aactgtaggc tttataaaga
    12901 agataataaa ttactcatag atactttaag agatgatgca aaaataagca ttagagatat
    12961 gaagactatt aaagtagata agaataaaca aattagtata tttgattaaa ataaatatac
    13021 taattacctt aatatatcaa gaatatagat acttttgata acaaaattaa acttaataga
    13081 tattttctat ataagaaaga tggtcataat atgtcaattt gaccatcttt ttgatataat
    13141 taaaacacaa ataatttagt aacaaaggag gcagaacttg aatttattaa aaaaatatcc
    13201 tatacctatt gcaagtttga ttttgtcaat ttttacttta ggtaacttat tacaatctta
    13261 tagtgaatca ttacgtaatg taattggatt tataggattt attttgtatg caatttatat
    13321 agtcaaaata atattactta gaaagggtgt taaagagcaa ttagagaacc ctttaatatc
    13381 aagtacattt ccaactataa caatggcaac aatgatattt gctacatata taaaaccatt
    13441 atctttagaa ttaagtatta tgatttggag cataggaatt gtgggtcatc ttgtgttaat
    13501 agttttattt agtaagaagt tcttagttaa attctcaata aaaacggtat ttccatcttg
    13561 gtatatagta tatgttggga tagttgcagc tagtgttacg gcaccagcat ttaatcaact
    13621 tttgattggc aaaattgctt tttggatagg atttgtatct tatatagttc ttataccagt
    13681 aattttaaag agagtatgga taatcaagga aatgcctgaa ccatctttac caagtatagc
    13741 tatattagct gcaccagggt cactattatt gtctggatat attaattctt tttcagataa
    13801 aaataatgta gtattatact tgttattcat attatcaatt gtattttata ttatagtttt
    13861 gggatactta ccgaaattat taagattgcc tttttatccg agttttgctg catttacatt
    13921 ccctatggca ataagtgcaa tgggtatgaa actaatgaat ggttatttaa ctaaatcagg
    13981 tcattttata agttggattt catatgcttc taagttagaa gagataattg ctacagtgat
    14041 tattttatat gttttagttt tatttattat atatcttagt aaatctgaaa ctacatctaa
    14101 agctaaataa gaattattaa atatgtgtat ttaataggaa tattgatata aatatatgaa
    14161 gataagataa accatagaaa cttaatttag cttaatttta ttggaggaaa acaaatgaaa
    14221 acaaaagaag aatatttaaa agaagttaga aacatatcag aagaatactt tagaaaagga
    14281 gagttttttt gctctgaagc agttcttcag acaataaatg atgctttagg ccaaccttta
    14341 agtccagaga taactaaatt agcatcagga tttccgatag gacttggaaa agctcaatgc
    14401 ctttgtggag cgatatctgg aggagagatg gctttaggta tagtatatgg aagagtacat
    14461 ggagaaacta tgaatccaaa gatgtttgag catgctaaag gtttgcatga ttatattaaa
    14521 aaagagtatg gtgcaacatg ttgtagagct attacaaaga aatgggatgg tgataacttt
    14581 atgtctcccg aaagaaaaga gcactgtatt aaaataacag gtcaagttac agagtgggtt
    14641 gcagataagc ttatagaaga tgaaaaactt aatatcaaat agtgtaatat tagttggtga
    14701 atttataaag ttaaatttac ttatagtata agatacttga tattctcgta ttgatagaaa
    14761 aatttatagc atatgtattt aatgaaatat acttatattt agagtgtggt ttaatttcat
    14821 aaatacatat gctattattt taagtatata agcaaattta tttaaataaa tagaaacagt
    14881 taatttatat gtacaaaact atatatgatt agtgtaaata aatacctatt ttataatact
    14941 caacataaag acaccagaca tgattagtat tataccaata aaaatagcaa gccattcttt
    15001 tttgttcttc ttttctttaa aaaatataat ccctcctaaa gtggctacta tcacacaact
    15061 ctgagagata gaaaaactta ttgtaactcc caatgtatta gtcgttatga acatacctaa
    15121 atttgctatt gaccacaata taccagtaat taaattgaat aaaacatttt ccctatatac
    15181 aatctcttta gaaaaatact tgacaataat agatgaagtt atcaacatac caacagattg
    15241 aggtaatata attctatatc cttcaattaa aaatatttga ttagttacaa catataatga
    15301 taaaaataga gatgataata atacaactcc aacacatttt aacatatttt tatctgtaga
    15361 ttttttagtt tgataattag tgagaagtat accaagtatt atacataata ttgataaaga
    15421 accaatcagt aacattttag aactggtcca ttcattaaac aagattacag ctaataatgt
    15481 cgtaaaagtt aattgtgaac catttgaaat tggcattact ttagatacac tagcaatttg
    15541 aattgcttta aactgcatta attgtcccaa actccagaat atgccagaaa tcatacttat
    15601 acaaaatgaa aaaaatgtaa tttcaggatg tataaccaga gttattatag tggaaaatat
    15661 aaacgcactt atggatgtac ctaaaagctg ttcatgagga gaacttttct ttagatttgc
    15721 aactatgggc atcaatcccc atcccattac aggaagtaaa gcaataatta ttgtcagcat
    15781 taaactattt cctttcatat taaatttaga cagcttttaa tttatcatct aaaattaaag
    15841 tgttcaatac ataaaaatta ccgtatttaa tcaggaaata ttagtagttt tttagaaaaa
    15901 cattttccat taaaataaag ataaagggaa ggagtaaaat tgaaaatgtt aactgatatg
    15961 attcaaaatc gtgaaattaa aataataaag tttttattaa aagaaggtca tacaactgta
    16021 aaagcaatag cagatgattt agatgtttca gaaaaaacag tttcaaaatc tcttaaagaa
    16081 gtagaagcag tgcttaaaaa gataggtctt tctttaataa gaaaacctaa gataggtgtt
    16141 tatattgatg gagatttaaa aaaaatactt ccttacctag ataataaagg aagtcaaatt
    16201 cctacaacca gagaggagag agttatctat atatattcag tacttttaaa gagtaatgat
    16261 tatattacta tacaacaact ttctgatgaa ctatatataa gtcgtggaac tattgaaagg
    16321 gatttatcag aagttgaaaa actgctaaaa aacgaaggga tttccatata taagaaacct
    16381 agtaagggaa taaagttaaa tataagtgaa agagataaaa gacaacttac tgctaagttt
    16441 atacataaat tttggagtag aaactggtat attaagcaag agcaagaaag cttttatcaa
    16501 gcatttgaaa agatacaggc agatgtaaat ggtatttttt cggaagatga tttatcaata
    16561 attattgata ttcttaggaa tttcagcaga gaaagaaaat ttgagttctc tgattatgaa
    16621 tttcagtcat tagttattca tttggcaata gctgtagaga gaattagaaa aggcgaatat
    16681 atagaagaaa gtttgtatat tggtaatata gaaaataatc aaatgtcaaa taccaattat
    16741 ttagcaaaac aattagaaga aaaattttca atattattac caaaatctga aattggatat
    16801 ataaatttac atttagtagc ggctgaccaa catagagata ctaatgaact aaaaaatact
    16861 tcaactaatc cttgtgaaac aagtacaaat aatatatcaa atcttcggga aattataaaa
    16921 aatacattgt atgagacagc ttatgacaag gatttaattg atggattgac ttcacatgta
    16981 cattcatcaa taaatagact gaaatatgga cttagtatta aaaacccata tgtaaataca
    17041 atcaaacaaa acttttcttt atcatttgag caagctttag atttaaagaa agtaattgaa
    17101 tttatatatg atattagaat aaatgatgat gaatgtgctt atatagcatt acattttgaa
    17161 gcttttagag aaagaatgaa agaacttcca caaaatatta gagtaattct agtatgcagt
    17221 acaggattag gttcatctag gttattgtct gcaagaataa aaaaatactt tccaatgata
    17281 aagattgaaa aagtactttc cgtacaagaa ttagtatcca gtaaaattga tgcagattta
    17341 gtaataagta ctatttattt agagttagaa acaattccaa ccatagtagt tagccctatt
    17401 ttaaatgaaa gtgatataag aaccttggaa agaaatatac aaaatgtttc atgttgtact
    17461 tctaagaagt ataaatcttt cagaaaccta atctttgaag aaaatatatt gttaaatatt
    17521 gaagcttcaa actatgaaga tgcaattaag tatataacag ataacctaat aaaaaaaggt
    17581 tttgtaaaag aaggtatagc taaaagtgca attgagagag aaaatttatc ttacacgtct
    17641 tttcagtcaa tagctacacc tcatgctaat cctagttata ttaaaaaatc taatatatct
    17701 gttgcaacgt taaaaaatcc tattacatgg ggaaatgaaa aggtggaagt tattttcttt
    17761 atttcattag aaaatgacaa aaacctagag cttgatgaaa tatatgaagt tttttatgac
    17821 tttattgaca ataaaaaaaa tataaatgac atattaagtg caacttccac aaaggaagtg
    17881 tacgatttgc tattggagga gagtatatag tgaaaatatc agatgtattg aaaaaagagc
    17941 aaattgtttt taatcttgat acaaatacaa aggaagaaac aataaaaaaa ttaagtagta
    18001 tatttgttga aaatgggatt gtggacaatg aagaagaata tgttaagtct gttttggaaa
    18061 gagaagaaca ttcaactaca ggtattggta atggtattgc tattcctcat ggaaaaagtg
    18121 atgctgtcaa aaaatcttct ataatatttg caaagactaa gcataagatt gagtgggatg
    18181 ctttagatga aaaaccagta gatagtattt tcttattggc tataagcaat attgataagg
    18241 atagcaatca tcttgtacta ttatctaaat tagcaactaa acttatggat gatgacaatg
    18301 tagatgcact aaaattagcc aattcagaac aagaaataat caatatattt agtgaagaag
    18361 gagagatgta gaaatgaaaa gaaaaattat agcagttaca gcatgtgcaa caggagttgc
    18421 acatacatat atggcagctc aagcgttaaa gaaagcttca aaaaagatgg gtcaccttat
    18481 aaaagttgaa acacaaggag ctacaggaat cgataatgag ttaactacaa aggatgcaca
    18541 aattgcagaa gtagtaatat ttgcagtaga tactaaagtc agaaatgctg aacgttttga
    18601 gggaaaagaa atactagaag taccagttaa tgcacctata aaggatgcag agaaagtaat
    18661 aaatgatgca ttaaagttaa ttgacactaa ataaatggag ggaaatgtaa tgttgaaaaa
    18721 gatagtctct gagttaaaaa agcatgtctt aactggaatc tcatatatga ttccattagt
    18781 aattgcaggt gctatgataa tggcaatatc tcgtgtaggt ggttctttct ataatatacc
    18841 agatatatgg gatgctaaat acgcagaaag tgctagtagt attgttagat tattacatga
    18901 tctagatgga tttggaggta ctgcattagg attaatgttt cctgtaattg ctgcatttat
    18961 aggattttct atagctgata aattagctat tgtaccaggt cttgttggag gtatgattgc
    19021 taaagatata ggtgcaggat ttttaggagc tttggcagtt ggtcttatag caggatatac
    19081 atgtttgttt attaaaaatc atgttaaatt accaaaatca gcagcttcaa tagtaccaat
    19141 atttatagta ccagtatttg gaacattaat cactgttgta ttaatcaatt atgtgatagg
    19201 tatacctttt gccacattaa atactggatt agaaaattgg ttaaatggat tatctggtac
    19261 aaatcagata ttaatggcag ctattatagg ggctatgatg gcagttgact taggtggacc
    19321 agttaataaa gcagctttaa caacatcact tgcattatta acaagtggaa tttatgcacc
    19381 taatactgct gcaatggtag gaatagttat accaccacta ggattgggac ttgctactat
    19441 actagcaaaa caaaaatata ataaacagtt acgtgaagct gggaaatcat ctcttataat
    19501 gggtttaatt ggagtaagtg aaggagcaat tccttttgcg gtagaatctc cattaaaagt
    19561 aataccttca tgtgtaattg gaacagcaat tgcttcagct atggcagttg gtttaggttc
    19621 agtaaatgca actccaatta gtggatttta tggatggttt acagtagaga attggccaat
    19681 gtatgtgtta tcaatagcag ttggaacagt aattgtagct ggattagtta tagtattgcg
    19741 tggaaatcca gtgattgagg atgatgaatt tgaagatgat gattttgatg aagcagagtg
    19801 ggaatcttaa aatttaataa ctatttaaga cgtgttataa gctatattca atatgaatct
    19861 tatactaaat aaagtgttaa atattcagtt tagacttaaa tataaaagat aaagaacaga
    19921 ggagaataaa tgataaaagc acatattgtt aatcatacac actgggatag agaatggtat
    19981 tttacttctg gagatgcatt agtacttagt gaacaactat ttacagatgt aattgatgaa
    20041 ttagaacgta atccagatgt aagttttgta cttgatggac aactttctat tttggatgat
    20101 tatgtacaat tacacaaaga aaaaatagaa atcattaaaa agttaataaa agaagataga
    20161 ctacatattg gaccatggtt tactcaaact gatgcattct ttgctagagg agagtctatt
    20221 ttaagaaact taatgattgg aatttttgaa agtaaaaagt atggtaagta tatgaaaatt
    20281 gggtatttgc cagatacatt tggatttaat gcacagatgc ctatactatt aaagcatgta
    20341 ggtcttgata atatattctt ttggagaggt atacatcttg gtaaacatgt tcaatcacca
    20401 tattttaagt ggaaaagctt aaatggagaa agttatatat atgcagtaaa tatgccacat
    20461 ggatatggaa ctggtatgct tttagagcct tcattaaagt atgttaatgg acgcttagac
    20521 ccagctattg attttattaa atcatatact gatgtagatg aggtgttaat accttctggg
    20581 aatgaccagt taaatataat aggtgatttc aaaaataaaa ttaaagcgat taatgatatc
    20641 ggaaagtatt catatataac gagttcatat caagagtttt taaaatatat aaagtccata
    20701 gaatcactag aaagctatag aggtgaattt agagaacctg tattagcaag aatacataaa
    20761 tcaattggtt ctagtagaat ggatataaaa ttagcatgtg ataaactaga aaacaagctg
    20821 attaaaagaa tagagccatt attagtaatt gctaaaaaga gtaaaattga aataagcaat
    20881 cagttactta taaatacatg gaaaaaactt ttggaaggac aagcacacga tagtctagct
    20941 ggatgtgtaa ctgatactgt aacagattat attcttcacc gtataagaga agcaaatgaa
    21001 atatgtgaca gtattgaaaa tactattgta aaaaagattt cagaagggct taaattatct
    21061 aaaaatgata ttttagtatt taatacagaa gctaagcgtt ttaatggata taaagagata
    21121 caagttgtaa gtgatagtaa aaatatttat tttaaagata atttagatgc tactataatt
    21181 gaagaaactt atgtaaaacc gagagaaaat gtattagaag aaacacctgc tggcaacgta
    21241 tttattgaag aatcaggata ttatatatta aaagtaagaa taaatgtaca gcttcctgca
    21301 cttggttata aagttgttag ttttgaatca agtgataaag aaatggtatc acttgataaa
    21361 tctaatgaca cttttatatc taatgataat tataagataa tctatgaaga tggaagctta
    21421 aatttacaac ttaaagatgg aacttacata tcaaactttt taatattaaa agatagtggt
    21481 aatgcaggcg atacctatga cttttctcca ttaaaaaatg acgaggatat aaaactttca
    21541 tttgatagag tgtatacaga aaaaacttta ggatatgaaa agttggtggt taaaggtact
    21601 actttactac cacttacatt agaagataga aaaaacaaat atcttaatgg gaaattaaat
    21661 gtaaaagtaa gtattgtctt atccaaagaa aatccattga tggatgtaaa actttgtgtt
    21721 gataacacga tatataatca tagactacgt gttcatataa aaactgatat aaaagataat
    21781 aaaaacatag catcattgcc atttggatat ataacaagag aaaatggagt tttagacaac
    21841 tgggaagata tttacagtga aatgcctata gatttagaac ctttggaaag taatattact
    21901 ctaacaaacc aaaatagaag ctgtactgta tttactagag gaataaaaga gtatcaacat
    21961 attgaagatg aaattgcatt gaccttactt gctactacag atgaactagg aaagcctgat
    22021 ttattatata gaccaggacg tgcttctggt gatacaacta aaaaaggtca tataagaatt
    22081 aaaacagata aagctcaaat gcttaagaaa ttagaatttt catttgcaat atatatggat
    22141 tctaaatcat ttgatgaatt ggaaattgcg aatttaaccc acaattatct aagggaatgt
    22201 gtaaattatc aattacagga ttacaatttc tttttatatc gtatagataa taagattcaa
    22261 aaatcaattg tcaataaaac tgtaagtaga gaattagaaa taatatcttt accagaaaaa
    22321 tatcttatat cagcatgtta tccttcttat tacagtaaag ataaatttat aataagagtg
    22381 gaaaatccaa gtagtaatag aatgatattg gatgagcaaa tatttaaaaa tagaaatggg
    22441 aaaattgtaa atgcaataga agatgtagaa gaagaacagg tatatgaaat agcacctttt
    22501 gacgttattt caatattatt ggatttataa gtgtaaaagt aagtttattg tttaaagtct
    22561 gacagatatt aatctgccag actttttaat ttgtataaat ttgggtaaat attaatgaat
    22621 agttttagta tttaaatatg aatttagtat ttaatataga atttattgaa gtagaataat
    22681 tagtttaaca ggataagcag tttaaataaa gaattattat attagagtga ttttatatat
    22741 aatttaaaat aataaaatgt taataaaatg
//
LOCUS       DAELXX010000065        22644 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.065, whole genome shotgun sequence.
ACCESSION   DAELXX010000065 DAELXX010000000
VERSION     DAELXX010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22644
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.065"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1426
                     /locus_tag="KPH29_002290"
     CDS             <1..1426
                     /locus_tag="KPH29_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBG1467879.1"
                     /translation="IKPLKISIKKSTEPLIEFISDTTYKNKSSSYIKEVHFTRYIGVD
                     DSIDKYIEKVKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYDSSKFNSW
                     DLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWMDYFL
                     PKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYPECSK
                     FSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLTSKKI
                     AQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVVNDGY
                     SFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIVAIGS
                     PLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAGYDDG
                     QNLNLAVESNLVKIFANNFIEIVN"
     gene            complement(1518..3392)
                     /gene="asnB"
                     /locus_tag="KPH29_002291"
     CDS             complement(1518..3392)
                     /gene="asnB"
                     /locus_tag="KPH29_002291"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBG1467880.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEYVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            3595..4269
                     /locus_tag="KPH29_002292"
     CDS             3595..4269
                     /locus_tag="KPH29_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBG1467881.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            4382..4918
                     /locus_tag="KPH29_002293"
     CDS             4382..4918
                     /locus_tag="KPH29_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBG1467882.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(5084..5704)
                     /locus_tag="KPH29_002294"
     CDS             complement(5084..5704)
                     /locus_tag="KPH29_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBG1467883.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(6018..8156)
                     /gene="vanT"
                     /locus_tag="KPH29_002295"
     CDS             complement(6018..8156)
                     /gene="vanT"
                     /locus_tag="KPH29_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBG1467884.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            complement(8174..8980)
                     /locus_tag="KPH29_002296"
     CDS             complement(8174..8980)
                     /locus_tag="KPH29_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG1467885.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            complement(8977..10077)
                     /gene="vanG"
                     /locus_tag="KPH29_002297"
     CDS             complement(8977..10077)
                     /gene="vanG"
                     /locus_tag="KPH29_002297"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HBG1467886.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            complement(10338..11420)
                     /locus_tag="KPH29_002298"
     CDS             complement(10338..11420)
                     /locus_tag="KPH29_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG1467887.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            complement(11470..12171)
                     /gene="vanR"
                     /locus_tag="KPH29_002299"
     CDS             complement(11470..12171)
                     /gene="vanR"
                     /locus_tag="KPH29_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HBG1467888.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KDLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            complement(12605..15136)
                     /locus_tag="KPH29_002300"
     CDS             complement(12605..15136)
                     /locus_tag="KPH29_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG1467889.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVKEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            complement(15244..15861)
                     /locus_tag="KPH29_002301"
     CDS             complement(15244..15861)
                     /locus_tag="KPH29_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBG1467890.1"
                     /translation="MCKKTYLLVVSILIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIITPQQAMEIALKEQNG
                     MVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            complement(16223..17107)
                     /locus_tag="KPH29_002302"
     CDS             complement(16223..17107)
                     /locus_tag="KPH29_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBG1467891.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLVLSKRDIDLTLRRFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAVKFATRVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            complement(17298..18548)
                     /locus_tag="KPH29_002303"
     CDS             complement(17298..18548)
                     /locus_tag="KPH29_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG1467892.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFIYIIVRDTPKEYGFNVDTEPYEKSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVDGYKKGFVV
                     FIVLSIITIVSSHIGVKNDDCKAI"
     gene            complement(18770..19534)
                     /locus_tag="KPH29_002304"
     CDS             complement(18770..19534)
                     /locus_tag="KPH29_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1467893.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTNMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            complement(19528..20253)
                     /locus_tag="KPH29_002305"
     CDS             complement(19528..20253)
                     /locus_tag="KPH29_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1467894.1"
                     /translation="MDKNISNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            complement(20255..20623)
                     /locus_tag="KPH29_002306"
     CDS             complement(20255..20623)
                     /locus_tag="KPH29_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG1467895.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            complement(20799..22343)
                     /locus_tag="KPH29_002307"
     CDS             complement(20799..22343)
                     /locus_tag="KPH29_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG1467896.1"
                     /translation="MRNLQKKLMFFLFICIILQITYTTPVYSLCKLYKNNSINNFESI
                     VSNNQFISKYAIQIILIMAIICMMLIFYIIYDKLNFKIKLQKIAYTDNLTGANTIDKF
                     VIDANKILCKNTQVKYALLYIDIDKFKYINDLFGYEVGNEILRNLTKIIKSNIFEEEM
                     FARISADNFIIIMKYIEEEDITKRLKTIFEELDLFNNNQEEKYKLVLSCGIYFILPED
                     RDINSIIDRANIPHKMAKGGHKSSYAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIY
                     LQPKIDLNTGEIQGSEALVRWKRPDKGLIPPNEFIPFFERNGFVINLDLYVLEEVCIY
                     LRKWIDAGINPVTVSVNVSRIHLYCNNFIETYKNIIDKYNIPAKYIELELTESIIFDN
                     FDILIDIMNNLKKIGFLISMDDFGSGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSK
                     IIVTKVIEMAKELGMKVISEGVETEEQFKLLKEVKCDMAQGYLFGKPMPIEEFEHLIV
                     SNLVRG"
BASE COUNT         7846 a         3819 c         2350 g         8629 t
ORIGIN      
        1 tattaaacca ttaaagattt caataaaaaa gtcaactgaa cctctaattg aatttatatc
       61 tgatactacc tataaaaata agagttcttc atatattaaa gaagtccact ttacaagata
      121 tataggtgtt gatgattcaa ttgataaata catagaaaaa gttaaaaata tagatactta
      181 tttttttaat aatcctacaa ttccctatat tagattaaac aatctaaaaa taaattttga
      241 caaagaacaa acagataaga tgcttaaaat ttttaatatt tatgatagtt ctaagtttaa
      301 cagttgggat ttatataata tcgactttcc atgtaaaata gaaaatgaca ctttaaattg
      361 gactaaaaag attgcattta aaaatacttt agatttattt tccacaagta tgccaaattg
      421 tacatcctct atcatcaaaa actttgcagt taaacttttg tgttggatgg actattttct
      481 acctaaatta ttttataaca aggtgaagac tactatttca ccaaagatag tatactttgg
      541 aaatattaaa aggcaagaac tttttttctt atatttttta tcacaacttg ggtgtgatat
      601 tttatatata aatccaaata aagatattct tgatttatat cctgaatgtt caaaattttc
      661 tactctattt gaactttcta ggaaaactcc aaatatatta gaaatacctt ttgaaaggca
      721 tattacaaat ataaattaca gtgaaaatac caataagact tctcctacta cgaataaacc
      781 taactctaaa aatacactta ataacacaaa tctaacatct aaaaaaattg cacaagatga
      841 ggaagtagaa ctttcttatg aagaccttgc aaagttatca gcttctgttg ttatgattgt
      901 tgtgtgcaat aacgaaaata aaccgtttaa gagtggttct ggagtagtaa taaataatga
      961 aggttatata cttacgaatc ttcatgtagt caatgatggg tattcatttt tagttagatt
     1021 tgaaaatgat gataaagttt atacatctta ccaaattata aaataccatt cagattatga
     1081 tttagctgtg ataaaggttg atagaaaatg caaaccaata cctgttaaag tcagtaaaaa
     1141 acctgtgaga ggtcaaaaaa tagttgcaat tggaagccct ttaggtcttt ttaatacagt
     1201 ttcagatgga attatctcag cttttcgtga ttttgaaacg gttcaaatga tacaatttac
     1261 agctccaatc tctagtggta gttcaggagg agctctttta gatatgtttg gaaacttact
     1321 aggattgatt tccgctggat atgatgatgg acaaaactta aatttagctg ttgaaagtaa
     1381 tcttgtcaaa atatttgcca ataattttat tgagatagta aactaatcaa tcaaaattct
     1441 acttatccta ttccataata atcaagaggg tgaaaacatt gcatgctttt gccctctgat
     1501 taatattgaa aaaagtatta atactcgaca tttttatgct caataaataa ttgatgccat
     1561 agacaaaatg taaatatagt ccatattttt ctcgaattat ctctatgacc tttaagatgc
     1621 gtatctaaaa gttttattgc atatttttta tctataaatt catctacatt tgaattataa
     1681 atagtttcct ttacaatatc ccctagagaa tcttttaacc aaactcttat tggagttgga
     1741 aatcctaatt ttttcctttg aacaacatat tctgggacta tttctctaaa cgcctctcta
     1801 agtaaaactt ttgtattatt cttatttatt ttttgctcca ttcttaaatt ttttgctaca
     1861 tcaaatacat ctttatataa aaatggtact ctaagttcaa cctgctcagc tgtagaaagt
     1921 ttgctaattt tttgaagtat atcgccttga agccatgtat tcacatctat atgttgcatt
     1981 ttacttatat aatcgtaatt atttttttgt gcatctctat acaaattaga caacaggtat
     2041 tcatatatat tcttttcctt atacttaaaa aagaatcttt ttacttcgct gttttcaaaa
     2101 acttttgcat ttcctatata tcttttttct aatggtgttg tagctctata aagataattt
     2161 cttcctttta tgtttggcat aagttctgaa actcttccta atatactttt aatataagta
     2221 ggtgaattga caactgattt catagtacta tactctttat atatattata tcctccaaat
     2281 aattcatctg ctccttctcc agacaatgct actttaacat gtttacctgc ctctttaatt
     2341 aagaaataaa gacctacttc tgaagggtct gctatagggt catccaaaga ataaattaca
     2401 ttcggtaatg attttatata ttcatcttgt gttatattaa tctggatatt ttctatacct
     2461 aacttatcag ctgttttctt tgctacttct aattcactat atcctttgac actaaagcct
     2521 gcagaaaaag attttatatt tggattaatt tgtgaagcca cagtagttat aatagatgaa
     2581 tctattccac cagacaaaaa tgttccaatt tctttctcct cttccatatg agttactatt
     2641 gaatctacta ctatatttct tatatcatcc tcattcacat ctctattgcc taagtgattt
     2701 ttagtataaa aaaattttgt actacttcta aactcaagtt tattatatct ctttaaagat
     2761 agtataccat tttctaccct aaaatagtga cctgctggta tcagccttat tccttgtatc
     2821 atagtatctt caggaagtac atattgaaat gaaaaataac tttgtaagct tctttcattt
     2881 atatttaaat ttttcatata ttcaagtaaa aacttatact ctgaggaaaa aattattgca
     2941 ttttccttat ttatataata aagtggtttt atcccaaata aatctcttgc tccaaatata
     3001 ctttcatttt ctctatcata gattataaag ttgaacattc ctttaatctt atgtacacaa
     3061 ttcttaccaa attctatata agatgccaat aagacttcaa tatcatcatt tgtagtaaaa
     3121 ttgtgtcctt ttttaattaa ttcttctttt atttcatgat agttatatat atttccatta
     3181 aaaacaataa cttttccatc tttttcaaat ggtttatttc cataaatagc atcaattaca
     3241 ttaaatcttt caaaatcaaa agttttttcc tcatccttat actctattct actagaaagt
     3301 cttgtattat ttacagattc ttttatttta aatttaatat cttctttttt agatatatca
     3361 cctttataat aaattgaaat attgccgtcc atataatcaa aattcctttc tgtcctttag
     3421 tcttattcta tatttcatag ttttgtcttt ggctttaaaa cttattcaat ttaaaatgaa
     3481 attgctatta aattcttata aattatctgt taatatttca tatttttttt atttctagaa
     3541 atactatatt aggaggtgat ttttatgaag aaaaaaatat taataccagt tattatgtct
     3601 ttatttataa tctcacagtg cataacttca tttgctttta cacctgaaaa taacaaattt
     3661 aaggttaaac cattacctta tgcatatgat gcacttgaac cttatataga taaagaaaca
     3721 atgaaactgc atcatgataa gcattatcaa gcttatgttg ataaattaaa tgctgctctt
     3781 gaaaaatatc ctgagcttta taattattct ttatgtgaat tattgcaaaa tttagattct
     3841 ttacctaaag atattgctac aactgtaaga aataatgcag gtggagctta taatcataaa
     3901 ttcttttttg atataatgac gccagaaaaa accatacctt ctgaatcttt aaaagaagct
     3961 attgatagag actttggttc ttttgaaaaa tttaagcaag agttccaaaa atctgcttta
     4021 gatgtctttg gttctggttg ggcttggctt gtagctacta aagatgggaa attatctatt
     4081 atgactactc caaatcagga tagccctgta agtaaaaacc taactcctat aataggactt
     4141 gatgtttggg agcatgctta ctatttaaaa tatcaaaata gaagaaatga atacattgac
     4201 aactggttta atgtagtaaa ttggaatggt gctttagaaa attacaaaaa tttaaaatct
     4261 caagattaat taaatattaa aatccttttg aaaaaaactt atgtttgcta tataatataa
     4321 taaaagatag tctaaattta ccaatataat gtttatagtg acgattcagg aggattttta
     4381 tatgataaaa gaaacacatg aaaggggagg ttatatattc gcacttttga tactcccctt
     4441 tataaacaat ctgtacctag ttaaatacga tattgcatat aaaatagtat taattataat
     4501 ttatatttat tttgcatatt taggttcttt atttccagat atagatatga gaggttctta
     4561 cataagtaaa aaatttatgt taatatacaa attatttgga agtaggttta gacatagagg
     4621 gtttactcac agtctaattg ccttattact tatttatagt ttttttaaat ctctgacaat
     4681 cttcactaac aataatatag ttttctcatg tttgtccagt gggtttataa taggatatct
     4741 atctcacttg tgtcttgacc ttattactaa agaaggtatt gaattgtttt atcccattac
     4801 cataaacatt tctttactac ctataaaaac taactcaaaa acagaaaaat tcatatctaa
     4861 gctattaaat ttcatagtta tttttttgat tggatatcaa ttttatattt tattatagtt
     4921 gtagaaaaaa taaataacaa tattttgaac taagtataaa taagttttat atattttagt
     4981 ttacaaaaga actaaaaatt ttgtacctta atacaaaaaa taaaacgtat cttaaaataa
     5041 tttagtacta aatcatttta agatacgttt tatttcgtac aagctaaatc attttatcat
     5101 ctttatcatt gtgatgtttt ttatgatgga aatcataatt atgattttca agatttttat
     5161 ttactctatt tccaatcttc catgacaaaa acaccaataa tataaatgat gctatcttta
     5221 aaggacttgt tataaaacct tctatatcac ttgccacgta acttataact ataaacatta
     5281 caaattttcc tgccaacatt gctggcacaa aatcttttaa atctttttta ccaaaagctg
     5341 atgcgatagt aactaaacaa ccaggcataa atggacaggc atacgctata aataataact
     5401 taaatccctg tttatccatc caacttattg ctttttcagt tttactattt ctcaacttat
     5461 taaaaagctt actatcatta aatctgctta ttaataagaa taatgaagtt gtcccaagtc
     5521 ctgagccaat ccatgataac aaacatccaa taaataatcc ttgtattgca gcatttgctg
     5581 taactatagc aacaagtggt aaggctggta taaagctttc tataaatgca cttaataagc
     5641 cgattattgt ggctaacacc caataatttt gagtcaaatc aagtaattct tgtatatact
     5701 gcatattgct tttccccttt tttaacttta actattatta tatactctat atgtttatta
     5761 tataccaatc tcaaaaaatg aatctaatat taaaaagtaa ataatctata taagtttaca
     5821 gttaaggtat aattatttta ttatatatta taacaaaaaa ggtgatgttt caaaataaaa
     5881 atctgaaaca tcaccttttt ataacttaaa ttgtcatgta agttataaaa aatatatatt
     5941 agacataata ttactaaaaa tgtctagtca gagtaaaata aattacacag tacaacaaat
     6001 attactattt aattgagtta cttctttata acatttaacc ttcttcctac tctgctcaat
     6061 aattcattcg atatactaca tgagttattt gcaacttcag aagcagataa ttcagataaa
     6121 ttatctcttc ctattattat tgccgtatta ccaacttcta cattaggtat atctgtaata
     6181 tcaacagcaa gttggtccat gcaaattcga cccactattg ctgctctaca accatttatt
     6241 attacgtaac tttttccaca ggataaattt ctaggtatac catcggcata gccaatagat
     6301 aatatagcaa ttacgctatc tctgcttgca ataaagactc ttccatatcc aaaactatca
     6361 tctttttgta tttttctaat aagaattact tgtgatttta aggataaaac aggtcgtaaa
     6421 tcaagttgca atttagtcgt atcatttgga gaacttaata tcccgtataa tgcaattcca
     6481 attcttacat aattacactt aagattggga taattaaaaa aaccataact actttgaata
     6541 tgtactttag gaatttttat accttttttt aacagaaaat caattaactt ataaaaacat
     6601 tcaatttgtt tatttgtaaa gtctacatct tcatctttca gactatctga aacacacaag
     6661 tgtgtaaata tgccacatat atctaagtat tttaatttaa atgcctttaa aattgacctc
     6721 acatcattaa catcaaaacc cagtctgtgc atacctgtat caattttaat gtgtgatttt
     6781 atgttgtatt tctgtttgtt taaagagata gcatgattaa ggtcaataac actctgtgta
     6841 agattgtatt tatgaagttc ctttgccctt attgggttag tatatccaag aatcaatatt
     6901 tctcctagta ttccatatcg acgtaaatca atcccctcat ctattgttgc tactgcaaat
     6961 gcttttacac ctatttggtt tgcacaagtc gctacttcaa aagcactatg accataggca
     7021 ttagctttta caactgccat caattcacaa tcttctggca tagctttttg tatcatcttt
     7081 acattatgtt ccaaattatt taaatcaatt tcaatccaag ctctattggt atttacctta
     7141 tgttctcctt tcaagtcttt ttttagaggt ttaagaatca atataataat tatagaagct
     7201 ataaaagatg ctatacttac cactatataa tgaataaggc tattgtcaac caagacagtt
     7261 tgtattccga atattttaga aaacatacga ataacaacaa tcatcatagg atgtataata
     7321 taaataatta atgatgatgt tcgcaataac ctagctcgag aaccttttaa aaatgttaag
     7381 gctgtaaata agaaatacat acatggcaat aacataatat acatgctatc atgacgctgt
     7441 acccctagtt tatgtaaaaa cattccctca cataacataa gtaagaatga tataagaaat
     7501 ccaatcaagc tatttttagt tgacatataa ttagatttat tagatattat tccaccaagt
     7561 atgaaaaata caggtgcaaa aaatatacca tttcgtgtat aatctgatac ttcaaaaata
     7621 tacccatata aattctttaa tagtggtatt ttttctaaaa aaccataata actatcacca
     7681 aacataccaa ttacataaat aacaatagtg attactaatg agtactttaa acctagtttt
     7741 tttacaagaa agtatgcaat acttgcacca ataattgatg caggtagata ccataaatga
     7801 tataaagtcc catcaaatac aatgtcctta ataatattgg gcactaaatt attcatggaa
     7861 aaatatccag tgtaaatatt taagggaata taaattacaa tagctatccc gtaaataaat
     7921 aatacatttt ttagaaaaga tttcagttta ttacaattat aattatatgt tgaaattaga
     7981 aaaaagcctg atagcataaa aaagaatggc acagcaatac gagcaattat actagttaac
     8041 acaaagtcag ccattttact atatgataat aatggagatg tatgaattgc aattataagt
     8101 aatgcagcta taatcctaaa ataatcaagc cctgcataag atttattcct aatcattctt
     8161 gttcctccat ttcttagtcc tttctatttc tccaaaatgt cacaataaaa ccatccttat
     8221 tgttacccga tatttgatat acacattgtt caggtataga gattctcact tcttctttag
     8281 tatgaatagg cacataaaag atttctataa ttttttcttt atcttctatt tttaaacaat
     8341 ttgtatacga aaatctctta atatattcta tatattcttc aagggcgaga ttcattttat
     8401 tgataatctc agaatgtgga tatccgacat aacgaaaatg ccatggttct tgagatattc
     8461 ctgttatttc ttctttttta cactgataac gctctataaa accataatgt agtgctgcct
     8521 tacggaactc attacaaata ccattataag gaaattcagg gcagataaaa tctataacct
     8581 ctttcttcaa ccctaaatca atagcaagcc ctgtctgatg ttcactgtga tttggaagtg
     8641 ctacaaactt tttagtaaag tcttttccat tgtcacgcag agagcttgaa tatatatctt
     8701 gttgttcctc tgcagtacga tatccactta caggtactat ttcatttatt ccacatatac
     8761 tttttaaaat attttgtaaa atatttgtag cttcacgttt cataagaata cttggaaacc
     8821 ttgtatctac aggaattaaa tctatatctt cagatgtttt aactggtaaa aaagcattta
     8881 ctagaattag attcccatca taaactttat ctttagtcaa taagatagta tttgtcattt
     8941 tatcttcttt atttttatct aataaaatat tattcatcat tttatgtaca cctcaattat
     9001 cttatccaat atttcttcaa aagacagtcc aatacctttc atcatattcg gataacgact
     9061 atgagaagta aagccaggaa ttgtattgac ttcattaaaa ataatttcac cagatggagt
     9121 taaaaacata tctactctag caaatccaga acatccaaga gttctataaa taatttttgc
     9181 tgtctcttgt attcttttct cacaatccaa atcaattcta gctggcatat gaatttttga
     9241 acttttcaat gtatattttt ctgtataatc aaaaaatcct tttgacaatt ctatttcatc
     9301 tactcttcca acaagtaagt catcgtttcc taaaactgca caccctacct caaaaccatc
     9361 aatattttcc tcaattatta cttcattatc atgttcaaaa gccaagtcta ctgctacttg
     9421 taactcttct tttttataaa ttttactaat accaaatgat gaacctgcac gcacaggttt
     9481 tataaataat ggataagtta agtcttttga cagttcagcc acattaaatt tatctgagta
     9541 ttttagtaat attgattttg gtactttaat accttctgaa tgaacaagtt tatgtgattt
     9601 gtctttgtcc atacataatg ctgaagaaag agtaccacat ccaataacct gtatacctgc
     9661 caattcaaat aacccctgca ctgttccatc ttctccattt tttccatgca atacaggaaa
     9721 tacaaaatca agtttagtgt atttaatatc attttcacat gattcaatag cactatttgg
     9781 aatatgattc tgagaaacag catcaggaat taaaaattca ataataccat ttttgacacg
     9841 gttctgcgaa acaataactg gaattatatc agtatccctt tcaaaccaag tgttattctg
     9901 gatattatta taatctccaa tataatgata ccaattaccc tcccttgtaa ttccaattgg
     9961 tataatatca tatttttccg tattcaaatt ctcaagtact gcaaaagctg attgtaatga
    10021 tacatcatat tctgttgagt ttccaccaaa tataactgca atttttttat tattcatctt
    10081 tgattctcct ttgttgattt atttattttt atacaatcta cagaaacatg ttaaaatact
    10141 ttttctattt caaatccaat caaccttcac ttacaattgc atctgttcca cataatatat
    10201 tttcacattt ttctattgat ataattactg tatcaaaata aaagcactct tgttttgata
    10261 cagtaattat atcaataaca agagtgctct ttatttattt atcctaaaga atttcctatt
    10321 tttttatact taaattctta caaatttcct acaatcggaa taataacctt aaaggtaatt
    10381 atattgttct cgctatgagc tgttattgtc ccgttatgaa gctccactat ttctttagct
    10441 atagcaagtc ccaacccagc tctaccactg tttgaattac gagatgtatc caacctgtaa
    10501 aattgttcaa aaatacgctc aagtttttcc tctggaatcg tattgctatg atttataaat
    10561 ttaagcttta tataatcatc ctcctgtaca gctgttattt caatagtagt atcttcaaaa
    10621 ctataataaa cagcatttcg taataagtta tcaaatacac gttgcatttt attaacatca
    10681 caccttatca tcatatttgg tgtaacattc agtgtatatt ttaaattctt actcatcaaa
    10741 gtaggtttaa actcatatgt tagttgttca agcatacgag ttaagcttac tgtactgtat
    10801 tcaagtgata tatttgataa attaaacctt gtaatttcaa agaattcatt aattaaatct
    10861 tcaagacgct ctgccttatc caacgaaata gataaatatt tttttcgcag ttcttcagaa
    10921 atctgacttt catctcgaag tagtgtcaga taccctataa ctgatgtaag tggtgttttt
    10981 aaatcatgtg caagataaac aataagttca ttcttacgtt gttctgccat ttgtgtctca
    11041 aatttacgtt gttcaagtat atgtttaatt gagtttattt tcttttctat tgccaataac
    11101 tctggagata aagccacttc tccaacatct tctttgataa gagagtcaat accttgattt
    11161 atttcactaa aatattttgt aaaccaattt aaataaacac ggaatattat aaaaaacaca
    11221 atcaccatag ctagtatcat gaagagctcc atgctattac ggaaaatctg ctgatataaa
    11281 ttcaatgcag ctttatattc catacgaaaa gcattttcaa agaatgatac tatccagata
    11341 gaaaacttac catacaatat aaaagaatat aaaatccata tgaaaataat ggctataaat
    11401 gccattaaag ctgttctcat aaaaatttta tttctcactt gagtaaagtc aggattgact
    11461 ttaatatttt tacttttcaa ttgtataacc tactccccat acagttttta taaatttcgg
    11521 gtttttagaa ggttcatgaa ttttttcacg taatcgccct atatgagcca taactgtgtt
    11581 attattatca agatattttt ctccccatac agcttcaaac aattcttcag aaggaaccac
    11641 ttttccttga tgttcacata aataccaaag aatagaaaat tcaatcggtg ttaatgctac
    11701 ttctttccca aataaactac atttatgtgt ttctttattg ataatcaatc ctttaatatc
    11761 atactcatta acaattatcg actgttgttc ataagaatta ttatatcgca tatatcttct
    11821 taattgtgtc tttactcttg caacaacttc caaagggtta aatggctttg ttatataatc
    11881 atcagcacct atggttaaac ccattatctt gtctatatct tcaattttag ctgttaacat
    11941 aatgatagga taatagtatt tttctcgaat tttctggcaa atatgaaatc catcaatatc
    12001 aggaagcata atatctaaaa tagccatatc caaatcttta gattcaatac attctagagc
    12061 ttccttacct gtataaaact tgtatactgt aaaaccttca ttttttaaat atacttcaat
    12121 taaatctgct atttcttttt catcatccac aatcaaaata ttttcgttca tagattattt
    12181 ttcactctcc ttattatcga ggtcatcatt tataatattg gtaaatttac atatcaaata
    12241 attacaagca tatttgtaac taatttttat aagaaaagaa ctactacatc agaatgtgtt
    12301 gatgctacta ttggaacaca aaaactgaga ttaattgtta ttataaaaac tcccctaaca
    12361 cgtccttatt atcgcataac aggtattaac tttcaataaa aatatctatc agtcaaacac
    12421 aattcaattt attataccag agacaatcca tataaaaaac aattaaacct atgtatgtaa
    12481 ccacagacat tactaaaatt tccatgttat tttatataaa aattcaaaaa aggagttgaa
    12541 tttaaaattt gattagaaaa aaatcaattt tgagacaact cctttttatt ttaatgtatt
    12601 taaattatag tatatcagcc ttcataatat cctcaaaatc acctttttca gatattaatc
    12661 ttatcaattt aacagtacag cttaaatcat ttataagtat agctccaact aatgaaccat
    12721 ctttcataaa taatttttta tatattcttt gaccttcatc ttttgatgaa atttctttat
    12781 caaaattttg tatattacca atactaaata cttttacatt cattgcatct aatgaaactg
    12841 gatatacttc actttcaaat gtcaaattat ctccacttgc atttgctcct gctatttttc
    12901 ccatttctac tgaagatggc catattgcta aactaatatt tcccacttgt gcaacatctc
    12961 cacaagcata tatatcttta acatttgtct gtaatgtctt atctacaaca atacctctat
    13021 caatttttac agatgtatcc tgaaccattt gagtgtttgc tcttacacca attgaaaata
    13081 ctaccatatc acagtcaaca ctatctccat ctttaaatac aactttttga gcttttccat
    13141 caccttcaat accatcaact gctacaccca atcttacatc tattcctgta tctcttacac
    13201 attgttctaa tattttacta ccctctttat caagttgttt tgatagtatg ctatccatgg
    13261 cttcaactac tactacttct aatcctttta gtctaaattc ccatgcagct tcaagtccca
    13321 ataaaccgcc accaatcact acaactttct tagatttttc tagtgcttct tttacactat
    13381 ataaatcttt tatatttcta agagtaaaca cattttctaa atcatggccc ttaatcggtg
    13441 gtataaagtt tctacttcct gtagctatta caagcttatc aaatttaata gctccatcat
    13501 tcactataat agttttattc acttcatcta atttatctac attcgttcct aatattacta
    13561 ctatattatt tttatcatac caatctttat cttccatgta gaaatctgaa ccaagttctt
    13621 catttatacc atcagaaagt gcaggtctat agtaagtcaa ctcatcttca ttagaaatca
    13681 tagttatctt acaagtttta tttctttttc ttatagcatc tgctgcataa aaaccagctg
    13741 ctccatttcc tactattacg aaatattcat cagtatcttt tctaaaagta acttcatctt
    13801 cttcaacttc tataaattgg tcttccttag ctccacatac agggcataca ctaggagcac
    13861 tatcaccttc aaaaacttca ttacaaatta cacatttaaa tttcttagtt ttctttttac
    13921 ttccaaaagt agaatttaac ttatctagga atttacctgt ataagaatgt aatttatcaa
    13981 tctcttcatt tgatggttta aagttaacag caaaaggttt tactgctgtc attctaagct
    14041 gacttattct ctccatagca ttttctatac cttctccact ccatccatat gaaccaaata
    14101 ctgaagcaac ttttccacca tgaagaactg ggtttaacga tattaatata tcccatatag
    14161 gctttaacgc atctcctaat attgttggtg ttccaaataa tacaccttgt gatactgcta
    14221 tttttgccaa aatatcttct tgcttatgct ctattacatc aaataaattt actttatagt
    14281 tgctattttt ttcaatatat gcttttatag cttctgccat aatctttgta tatccatgtg
    14341 cagaaacata acaaattgta acttcttttt ctaactttat ttgttccttt acactccagt
    14401 tataataaag gtctataatt tttctaggat tttcagttaa tactggacca tgacctggac
    14461 atacagtatc tatatctaaa tcttttatct tttctatagc agttaccata gatggtttaa
    14521 atggacccat tatacaatca taatagtatc ttaaagcatc caagtaatcc ttttcttcat
    14581 tttcatcaag tgtatttact atcttatcat tactatagtg actaccaaat gaatcacatg
    14641 ttacaagagt tttatcctct tttatatatg tgtatatagt atctggccaa tgaagcatag
    14701 gtacagagaa aaattctaaa gttttgtccc caattgataa tgtatctcca tctgtaactg
    14761 caacatattc aaagtctttg tttacgattt ccttaagata tttaattgct gtttctgaag
    14821 ctactacttt tgcattttta gctaaatcca aaagcttttc aacgcttcct gcatggtctg
    14881 gttcagtatg acttacaaca atatagtcta ttttttcaaa atctatattt aaatcattta
    14941 atctttcaat ataattgtca aaatgcttat ctttaactgt ctcaaacaaa actgtttttt
    15001 ctgttccttt taatacatat gaattataag tagttccata aggtgtatac ataattatgt
    15061 caaatactct taaatcaggg tcaagtgaac ctacccaata taaattttct tttattttta
    15121 gtgatttcaa tgacataccg tactcctcct aaaatagtta tttagtctct ttataccctg
    15181 cttgttatat tttaaacaaa atttattcaa aaaagaaatt attttaaaga tagtttaaat
    15241 cttctaatct tctttttcta ccttcaatac agttcccttt ttagaatcta cttttatgtc
    15301 atattcattt ttatctttat ctattcttat tttataaaaa gtaacatcta aatctttgtc
    15361 taaactccat tctttaacca ttccattttg ttcttttaat gcaatttcca tagcttgttg
    15421 aggtgtaatt atgtcattta aatctaattt ttcttttcta gcaactccat ttaaatcttc
    15481 tgaatctaac ttttctgttt tatcttctaa tacatctttt gtattagcat caattttcat
    15541 cttatattca ttattatcat ctacaccttc cacagtataa acaaatttat ttaaatctct
    15601 ctcaagactt aaatctacta tatcagcatc cttgtattta tctttaaata tgtttattgc
    15661 atctgtgtat ggcactttaa aatcttgttc cattaaattt tcattatttt tttcatcttt
    15721 taaagcatta tttgaagcat ttgcctcctt ttttgtatta tcttgtgctt gtcctgaatc
    15781 cttagttggg ctcccactac atgcagttaa taaacctgct ataagtatac ttaccactaa
    15841 taaatacgtt tttttacaca taaaaatcct ccttaaatat attaatttat tctatattat
    15901 gccctctaaa ccctttattt acccttttat attaaaaatt cctcctattt tatcaatttt
    15961 aaaactttat atttactaaa attaaagtta cataaaaatt atactgtctc aaaatagaaa
    16021 caagcatctt tttcattgtt gaaataataa aaatatattt tatttcgtac aaaaagagtg
    16081 cctcatgaac taaaaagttc attttgagac actcccattc ttttatattt ttacttacaa
    16141 aataacagta cttattaatt ttttacaaac cttataagta tatctatatt atattcttaa
    16201 atttaaacac tatattaaaa aattaaattt tgtttaattc ttttactgtt aaatccattt
    16261 taggatatgg aatcgtaatt ccttcttcgt caaacctaac tttaacctct tctagtaaat
    16321 caagataaat tgtccaataa tcttctgtct tgcaccaaac tctagttgca aatttaacag
    16381 cattatctcc atgttctact actcctataa atggttctgg ctcatttatt attaatttat
    16441 gattattaac aatatcttta agtactcttc taacatgtag tatatcatct tcatacccta
    16501 cactaaaagt caaatcaact cgtctagtat tctttgctga atagtttatt agactatcat
    16561 ttgatacaga accattaggt ataagtatct gtttattatc tggagtaacc aattgtgtat
    16621 aaaaaagtcc tatttgttca accgttcctc catggcctgc tgcttctacg taatctccta
    16681 ctttaaaagg tctaatcaat agtataataa atcctccagc aaaatttgat agactacctt
    16741 gtaaagccaa acctattgct acccctgctg atgctatcac agcagcaaaa ctggacaaac
    16801 tcattcccca atattccaga acaatgatta taacggcaac tttaagtaca ctcaaaagaa
    16861 gagacttcaa aaaccttctt aaagttaaat caatatctct tttactaagt actaacataa
    16921 catggtttac aaattttttt attattttaa atccaataga aagtataagt atacctatta
    16981 taagttttac tccgcttgta gtagcccatt ctagcagact ttccattata cctcccatat
    17041 ccatcttatt aatgtctaaa ttatttaaat ctaacttgct tatatcatct attttcgtcg
    17101 tatccataaa aaactcctta ccttatattt tagaaccaat attatagttt aaagttctta
    17161 ttttctattt aatataatac aataccattt gtacatagta aacaagattt atattccaat
    17221 tatacatctt aggaaaatgc aaactatttg ttatatatat gcaaaatata atactaaatt
    17281 aaactattaa taaaacacta tatagcctta caatcatcat tttttacccc tatatgagat
    17341 gatactatag ttataatact tagtactata aatactacaa aacctttttt atatccatca
    17401 actatattat atgaatttac ttgtaaagag atagctataa tcaaattaat aattccactt
    17461 cctataaatt caaaagtatt aataacacta gttgccagtc cagaataaat ttctttattt
    17521 ttatacttgg catcattaaa aacctgtaga tgtgacataa ctatacatcc cataataaag
    17581 aatgatattg gtaaaactat aattggtggt tttacctggt atacaactac aattacaatc
    17641 catataaaca aatctatcat tgctccaaat tttattatat taaatttgct agaccttatt
    17701 ttagcaaata caaaatccat aattattgaa ccaaatataa atccatatgt aaaaaatgat
    17761 actataaacg cagaaaaact cttactcaca ccataaacgt ccattatgta tcttactccc
    17821 cacaagctta taaatgcact tgtaagacca acaaaagaga acattatcat tgaattatac
    17881 catgttgatt tgttctttat aacagactta ataccatcaa caatattaac cttctctgac
    17941 ttttcatagg gttcagtatc tacattgaat ccatactctt ttggtgtatc tctaacaata
    18001 atatatataa aacatccaat tacaacacca attataccga ttaataaaaa tgaatttctc
    18061 caaccaacaa gttcagacaa aaacacaagt ggaaaagtag caagaactcc acctaaattt
    18121 cctacaaatg aaaatttagc tgttgcagaa gcaaattctg acttattaaa ccatctccct
    18181 tgaattttta aaatacaaag taatattact gatgttcctg cccctacaat tacacgagaa
    18241 atatatgcta attctatact ttgaatcaaa ccaaataaaa ttgaccctaa acttgccatt
    18301 attattccaa gactgctaat ttttcttgct ccatatttat caatcaaaat tcctgcaggt
    18361 atttgcataa atgcatatgc atataggcaa aaggatgcta tatttgaaat ttgtatagaa
    18421 ttaaaaccta actcatttgc taaattgtct gaaacaacag cagttgacat ccttaaaaat
    18481 aatactaaaa caaatgaaaa tgtaattata ccccaaacac tccacttagc ctttgtataa
    18541 cttttcatat cttctccctc aaatccatat atttttaaat tttcttgata taacttatat
    18601 aaagtgtata gttattctta caaattttta cacgcaatat cattatacaa catatgtata
    18661 tatttaacta gaaataaaat actattcata ttatatacat gaattaacta cataaataat
    18721 aagagccatc ttttaacatt actcaaaaga tggctccata taaaaacatt tattctaaat
    18781 ttaatttttt atctaatata aattttgtag cgaaaaataa cgctattgca actacaagac
    18841 taccaagtat tgcaagcata aaactaggac tctgataaag atgatatacc atatttgtat
    18901 cttcatttgg taagatgctt ccaactgcat ctccaacaat tgatattact atatttatag
    18961 ctataaaaga agcaaatgcc actatgtttc tatgtttatt gaattttggt aattgtccca
    19021 ttgagattga tgtatataat aacaaaataa atgatgaata tgaaataaac attactacta
    19081 ctaataataa aatatctaaa aagttttcag aaatccattt aaaactagtg ttaaacaaat
    19141 ctaaaatttc aggtagtaaa attccagaag taccaaaaag cataagaact ccaaaagtga
    19201 atactgcaac tataaaacta agtacagcgt atataagtgc tgttatacat ttagataata
    19261 taagtgaact agtgctaaca ggaagagtaa acattaaata accttcatcc cccaaaagat
    19321 tttttctaaa cctttgaatt gtaactacaa tagttaaaac tcctaatgcc ataaaaagac
    19381 ttgttaatac tagtattcct attccctgta cctgaagtat atttgtattc ataaatattc
    19441 cattaaatac agctactatc aatatggcta tatataacgg tataaatatt cttccacttg
    19501 cttttaactc atattttaat aatttaccta acatttaaat tcctccctaa ataatgcatc
    19561 tatagatttt cctttttctt ctcttattgc ttcaacgtct ccttgtaaca ctatttctcc
    19621 ttttgatatg aatattatct catcacatat gttttctatt tcacttatta agtgggtagc
    19681 aatcagtaaa gtactatctt cagaataatt ttttaatata gtcttaagta tatatgacct
    19741 tgcagctggg tcaacaccac ctataggttc atccaatata tatatgctcg catttcttga
    19801 cattactaaa ataagttgaa ctttttcttt agttccttta gacattgtct ttagcttttc
    19861 atttacatca atatctaagc ttttaatcat ctcatttgct cttctaacat caaaatcact
    19921 ataaaaatca tagaaaaact taagtaaatc tgataccttc atccaatcat ttaaataagt
    19981 tctttctggt aaatatgaaa ctattttttt agtatctatg gatggtttca ttccatttat
    20041 cataatttca ccattatcag gctgtaataa tccattcatt agcttaatca ttgtactttt
    20101 accacttcca ttaggcccta acagcccgac tatttttccc tttggtatat ttaaatcaat
    20161 attttttaaa acattcttag ttccataact cttgtttaca cctttaaatt caactattcc
    20221 atcttgagaa acattgctta tatttttatc cattttataa ttctcctttc agattttctg
    20281 taataagttt tagcatttca tctctatcat atccaagctt ttctaaggtt acttttaatg
    20341 tatttatctc cacattagct atttcctctt tcatagcttt tattttttcc ttatcatctg
    20401 ttacaaatct tcctttagtt ctctgactat aaactaaacc ttgtctttca agttctgtta
    20461 gagccttttg cattgtattt ggattaactt ccgcatcttc tgctagtgct ctcactgttt
    20521 ctaatttaga tcctatttta atttcccctg aaattatttt taactttaaa tgttctacta
    20581 attgtatata tataggtttg ttattatcaa gttcccattc catgttcatg cctccttatt
    20641 atattattgt attaatttat taatacaata atataataat acaaagtata tgtcaattac
    20701 tttttagtaa aagtataaaa aatttcacaa aaaaaatgaa cactacatta gagtccattt
    20761 ttaacacaat ttattaaatt ctttatatta cagattaatt accctcttac caagttggat
    20821 acaattaaat gttcaaattc ttcaattggc attggctttc caaacaaata tccttgtgcc
    20881 atatcacact ttacttcttt taaaagttta aactgttctt ctgtctcaac tccttctgat
    20941 atgactttca tacccaactc ttttgccatt tctattactt tagtaacaat tattttgctt
    21001 cttttactat tataagtttc cataataaat ttttggtcaa gttttaagat atccatagga
    21061 atttctttta acatatttaa tgatgagtac cctgaaccaa aatcatccat agatattaaa
    21121 aatcctattt tttttaaatt attcattata tcaattaaaa tatcaaaatt atcaaatatg
    21181 atactctccg ttaattcaag ttctatatac tttgcaggta tattgtattt atcaattata
    21241 tttttgtatg tttctataaa attattgcaa tataaatgta ttcttgaaac attaactgaa
    21301 acagtaactg gatttattcc tgcatcaatc cattttctaa ggtatataca tacttcttca
    21361 agcacatata aatctaaatt tataacaaaa ccgtttcttt caaaaaatgg gataaattca
    21421 tttggaggta ttagcccttt atcaggacgt ttccacctaa caagtgcttc tgaaccttgt
    21481 atctctcctg tattaagatc tatcttaggt tgtaagtata taataaactc tttattttct
    21541 aaacttgaaa acattgtatt ttccatctct ttttctttta tctcttggtc atgaatctta
    21601 ttatcataaa atgcatatga acttttatgt cctccctttg ccattttatg aggtatatta
    21661 gccctatcta tgattgagtt gatatctcta tcttctggaa gtataaaata gattccacaa
    21721 cttaaaacta acttatactt ttcttcttga ttattattaa acaagtctaa ttcctcaaat
    21781 atagttttta acctctttgt tatatcttct tcttctatat atttcataat tataataaaa
    21841 ttatccgcag atattcttgc aaacatttcc tcttcaaata tattactctt aataattttt
    21901 gttaaattac gcaaaatttc attcccaact tcatatccaa ataaatcatt tatatattta
    21961 aatttatcaa tatctatata taataaagca tactttactt gagtattttt acatagtatt
    22021 ttgttagcat caattacaaa tttatctata gtatttgctc ctgtaagatt atctgtatat
    22081 gctatctttt gcaatttaat tttaaaattt aatttgtcat atattatata gaatataagc
    22141 atcatacata ttatagccat aataagtatt atttgtattg catatttgct aataaactga
    22201 ttattagaca caatagactc aaagttattt atggagttgt ttttatagag tttgcataaa
    22261 gaatagactg gtgttgtgta tgtaatttgt aaaattatac aaataaataa aaaaaacatc
    22321 aatttttttt gtaaattcct cataaaaatt tcctcccatt aaaacacctc tattttatta
    22381 gcaaataagt acatctcaaa agtttccatt tcttgtaaaa tggatagttt tgaaaagaaa
    22441 atattaaaat tataatataa aaataaaatt tttcagacaa ttcaaacaaa aataatcacc
    22501 ttttaataaa taattatatg ccaaaataat ccaatttatt ttctattata cctatatgaa
    22561 atatataaat caatagttaa gacaatgaat atgctacatt ctataattat agtagcagaa
    22621 aattttacaa aattaaagta gata
//
LOCUS       DAELXX010000066        22603 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.066, whole genome shotgun sequence.
ACCESSION   DAELXX010000066 DAELXX010000000
VERSION     DAELXX010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22603
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.066"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..404)
                     /locus_tag="KPH29_002308"
     CDS             complement(<1..404)
                     /locus_tag="KPH29_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HBG1467897.1"
                     /translation="MKNLNLLINKLYSKNHNEAYKTFLFLENESLKSNITYCFFDSFL
                     EMIDNENSYIRTRGLLLISANAQWDIDNKIEINIDSILSHIVDKKPSVSRMFIKSIPN
                     ITKYKENLINRIKMELSNADISIYNNNMKPLV"
     gene            complement(610..987)
                     /locus_tag="KPH29_002309"
     CDS             complement(610..987)
                     /locus_tag="KPH29_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HBG1467898.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
     gene            complement(1103..1597)
                     /locus_tag="KPH29_002310"
     CDS             complement(1103..1597)
                     /locus_tag="KPH29_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBG1467899.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSVYIGDEKFNNLKNECIKK
                     GIDIKISKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKNIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            complement(1746..5999)
                     /locus_tag="KPH29_002311"
     CDS             complement(1746..5999)
                     /locus_tag="KPH29_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBG1467900.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKISYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDVPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            complement(6380..7549)
                     /locus_tag="KPH29_002312"
     CDS             complement(6380..7549)
                     /locus_tag="KPH29_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467901.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYNPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            complement(7944..8960)
                     /gene="gap"
                     /locus_tag="KPH29_002313"
     CDS             complement(7944..8960)
                     /gene="gap"
                     /locus_tag="KPH29_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBG1467902.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYSDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNQKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            complement(9597..10061)
                     /locus_tag="KPH29_002314"
     CDS             complement(9597..10061)
                     /locus_tag="KPH29_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 family lipoprotein"
                     /protein_id="HBG1467903.1"
                     /translation="MKKLVIILISIISVVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNARLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTDETLWSNTWH
                     GSVDNDTFAISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
     gene            complement(10385..10993)
                     /locus_tag="KPH29_002315"
     CDS             complement(10385..10993)
                     /locus_tag="KPH29_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG1467904.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
     gene            complement(11075..11776)
                     /locus_tag="KPH29_002316"
     CDS             complement(11075..11776)
                     /locus_tag="KPH29_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467905.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            12003..12269
                     /locus_tag="KPH29_002317"
     CDS             12003..12269
                     /locus_tag="KPH29_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBG1467906.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSMIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFYILK"
     gene            complement(12372..12791)
                     /locus_tag="KPH29_002318"
     CDS             complement(12372..12791)
                     /locus_tag="KPH29_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HBG1467907.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERIFNLKQEKRKAKHKGH"
     gene            complement(13058..13840)
                     /locus_tag="KPH29_002319"
                     /pseudo
     CDS             complement(13058..13840)
                     /locus_tag="KPH29_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
     gene            complement(13905..14666)
                     /locus_tag="KPH29_002320"
     CDS             complement(13905..14666)
                     /locus_tag="KPH29_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBG1467908.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLARILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNKKLVIKAEKKVTKAAKKLKHGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            complement(15715..16527)
                     /locus_tag="KPH29_002321"
     CDS             complement(15715..16527)
                     /locus_tag="KPH29_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG1467909.1"
                     /translation="MYLERPELYKQSEINFWDDEYISKQLLKAHLDTNFEGASRNFNF
                     IEDSVNWIVTVANPVNYPNLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSINYAQNRN
                     KETNLNINYLFQSYLNMNYNDEFDLATLIYCDYGALSTENRRLLMEKIYDSLKPGGKL
                     ILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLEQTLVITEKD
                     ENVFYVWNHYFSKESFLLEVENIGFKSVEFFSNVKGEAYSDDSMTMGLVLQK"
     gene            complement(16897..17799)
                     /locus_tag="KPH29_002322"
     CDS             complement(16897..17799)
                     /locus_tag="KPH29_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG1467910.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSAGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDGQISFFD"
     gene            complement(17849..18310)
                     /locus_tag="KPH29_002323"
     CDS             complement(17849..18310)
                     /locus_tag="KPH29_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HBG1467911.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIMF"
     gene            complement(18362..18817)
                     /gene="def"
                     /locus_tag="KPH29_002324"
     CDS             complement(18362..18817)
                     /gene="def"
                     /locus_tag="KPH29_002324"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBG1467912.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIKQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            complement(18789..19052)
                     /locus_tag="KPH29_002325"
     CDS             complement(18789..19052)
                     /locus_tag="KPH29_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1467913.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            complement(19149..19865)
                     /locus_tag="KPH29_002326"
     CDS             complement(19149..19865)
                     /locus_tag="KPH29_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1467914.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGTLAFIITMILLIFFSLTKISFLLNLSISLLVLLQGVIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYIYMFISI
                     VISLIWILLLFKKFSKKIVK"
     gene            complement(19862..20539)
                     /locus_tag="KPH29_002327"
     CDS             complement(19862..20539)
                     /locus_tag="KPH29_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1467915.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLILASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANLYPFNICISLISGDKNFIMINILISIIIIIYFITYYFICSSWK
                     KVGGVKL"
     gene            complement(20536..21381)
                     /locus_tag="KPH29_002328"
     CDS             complement(20536..21381)
                     /locus_tag="KPH29_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1467916.1"
                     /translation="MIKVDDLSFSYTDRNFLQNINFEVEKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENEANKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIKKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            complement(21495..22085)
                     /locus_tag="KPH29_002329"
     CDS             complement(21495..22085)
                     /locus_tag="KPH29_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG1467917.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEHFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRVVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
     gene            22374..>22603
                     /locus_tag="KPH29_002330"
     CDS             22374..>22603
                     /locus_tag="KPH29_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467918.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPLSSIISIRTL"
BASE COUNT         7456 a         3830 c         2189 g         9128 t
ORIGIN      
        1 tctactaatg gtttcatatt attattataa atacttatat ctgcattact taattccatt
       61 tttatgcgat taataagatt ttctttatac tttgtaatat taggtatgga tttaataaac
      121 attcttgata cagaaggttt tttatctacg atatgactta gtatactatc tatatttatt
      181 tcaattttat tatcaatatc ccactgagca tttgcagaga ttaataacaa tccccttgtt
      241 cgaatatatg agttttcgtt atcaatcatt tctaggaatg agtcaaaaaa acaatatgta
      301 atatttgatt ttaaactttc attttctaag aataaaaaag ttttatatgc ttcattatga
      361 ttttttgaat ataatttatt aataagtaaa ttgagattct tcaatcttct catctccctc
      421 attacaaata tttatttaaa caaagtataa catattcaaa aataaatata aataaaaact
      481 atattaaata aaaatcctaa aattatttgc tgtttattta caaataataa ttctaaaata
      541 tatttgattt atttagtatc gactataaaa ataagctatc aaataattta tacttgatag
      601 cttattttct tataattctt tttttaaagt ttcctttaga ctatcatatg cgaaatccat
      661 tatttctcta caagcaacta aatctttttt taggtctata cataactctg aaccatactc
      721 ttttcttact gttgtagtta aatctttagc atatacttta gctttagtac tttcatcttc
      781 gtggtttctg ccttttacgt atccaattat tatatttgat gcatttactg caccacataa
      841 actagcaacc ccacaccctg aacccagtcc acttcctaaa caaactggta tatctgtatt
      901 aaattcctca ttatatgctt ttataactga ttctgcacaa ttaaatccct tctcatgata
      961 ttttgatgct ataactttat cacacattaa aattccccct catttttgta aattaaaata
     1021 aattcttcat tgtttttgta aaaaatactt ctttttacta ttattatatc aaatattaac
     1081 aataaaaaca tttctttcta agttaactct gtctttctaa atttaatagt atctctttca
     1141 aatcagaatg attccccata tatccaccaa tatttttttt gccaactact ctatgacaag
     1201 gtattattat tggaatttta ttacttttac ttgcttgtcc tattgctctt actgattttg
     1261 gattgtttat atttcttgct atatctgaat aacacaatgt ttctccataa ggtattttta
     1321 acatttccct ataaactgat ttttgaaatt cagtcccttc aatacgaata tttaaattaa
     1381 agacttttct ttttccatta aagtaatcat ctatctggct tattgcttcc tttaaatctt
     1441 ccttacttat ctttatatca attccttttt taatacattc attttttaaa ttattaaatt
     1501 tttcatctcc tatgtataca ctaactataa aaccatcttc acttaatatg tacaaatttc
     1561 ctaattttga tttataaaca tcatatacta aactcatatt acttcccccc tttaatatca
     1621 gtatacatat aattttttaa ctaataaaga gttaccctaa aaaataggat aactcttttt
     1681 tatagatact taatatagag tatattttta aattcattaa cttttaacat atcaaaatat
     1741 aaatcttata tatttttaaa agccttagaa agcataagtt ttatcctgtt aagttgattt
     1801 acctcacttg cagaagggtc ataatcaata ggtattatat ttgcattttt atacaacctc
     1861 tttaattctt ttatagaacc cttaccaatt atgtgattag gtaaacatcc aaaaggttgc
     1921 atacaaataa tgttttctac accttcattt aataattcaa ccatctcacc tgttaaaagc
     1981 caaccttctc cagtttgatt acctaatgac actactggtt ctgtactttt agcaacattt
     2041 tttatattgg ctggagcata aaatctctca ctcttatcaa gtgctttttt atatactctt
     2101 tgatatattc cagttatgta tataaatgct tctcctatca ttctactctt tctacttcct
     2161 tctaaatgag tgtgtttgta tatagtatta aaggcacaac ttaagaagaa atttgtaagg
     2221 tctggcacga ctgcttcagc accttctcgc tctaatatat ctactaaatt attatttgca
     2281 attggatgga atttcaccaa tatttctcca accaaaccta cttttggttt tttgatatct
     2341 agtatctcta aattatcaaa ttcttttact atatctttta tatttctagt aaatacagaa
     2401 atttttgctt taacaagaga agatttgcat atatcaaccc atttttcata tagagcattt
     2461 gcacttccag taactttttc atatggtctt actctatata gtactttcat cagcaaatca
     2521 ccataaacaa cagacataaa gagtctattg attaatttta gtgatatatt atccattata
     2581 ccactatctt ctattccatt tacactaagt gcaatgacag gaacatcttt aaatccagca
     2641 tcatatatag cctttcttaa gaatccaata taatttgtag ctctacaacc tccaccagtc
     2701 tgagttatag taacagaagt attatttaaa tcatatttgc cagattttaa tgctgttatt
     2761 agctgaccta caactattat agctggataa catgcatcat tatttacata tcttaaccct
     2821 tcttcaatta cactactatc agtatctttt agaatttcta tattataacc acttaagtta
     2881 acagcttttt caaggaattg gaaatgtatt ggagacattt gtggtgctaa tatagtatgt
     2941 ttgttcgtta tctgattatt tttagcatac tgtatcctgt ttacctttat attttttaca
     3001 tctatatttt tgctttctct ctcaaacata gctgctttta aggacctcat ccttatctta
     3061 gctgctccta aattgttgcc ttcgtcaatc tttataatag tatatatctt cgatttttca
     3121 ttaagtattt cttgaacctg gtctgtagtt actgcatcta atccacagcc aaatgaatta
     3181 agttgaacaa gttccaaata gtttttatct cttacaaaac tagctgcttt atacaatctt
     3241 gaatgatata cccattggtc tacaactcta agtggtcttt gtacattggc taaatgacat
     3301 atagagtctt cagtaagcac tgccatatct aatgaattta taagctctgg cattccatga
     3361 tttatttctg ggtctatatg atatggtctt ccacataaca caatcccctt catatttctt
     3421 tcttttattt ctttaagtgc tctttcacct gctgcttgta tctccatttt aaataaattc
     3481 tgttcatcag tagctttatc aactgcatga ttaacttctt ctttagttat ttttatatca
     3541 gaaaaatgtc ttgataattc atcatatagt cttttcttca atttttcctt atcatttaga
     3601 cttataaatg gatttatata atttatgttc ttatttctta tattctctac attgtttttt
     3661 attacttcag aatatgatat tacaacagga caattataat aattatctgc atttgaatcc
     3721 tctttatttt catttgttac acttggataa aatatatttt ttataccttt tgcaattaaa
     3781 ttttctatat gtccatgaac taattttcct ggataacaaa ctgtttcaga tgcaatacta
     3841 gttataccag tttcatataa ctgttttgat gacctatctg aaagtacaac tctaaagccc
     3901 atagcattga aaaaagtaaa ccaaaatgga taatcttcat acatatttaa tactcttggt
     3961 atccctattt ccccattttt tgcttcttct ttttccaatg gtttgtatct aaaaattcta
     4021 ttgtatttgt acttaaataa atttattggt tttttctttt ctaatcctat ttttttatca
     4081 ccataaatag cttcaccctt ctcacatcta ttacctgaaa taaaaatttc attatcagaa
     4141 aatttattta ttgtaagaag acaatgattt gaacaacctt tacatctagt tacacttgct
     4201 tctagcttga tattattcaa ctcttcctta gataacaagg atgttttgta tccactttca
     4261 tatttttctt tagctataag tgctgaacca aaagctccca taattcctga aatattaggt
     4321 ctaattacat tttttccaat tagtttttca aagcttctaa gcaccaaatc attataaaag
     4381 gttccacctt gtacaacaat attattacca atctcattta aatctctaat ctttattact
     4441 ttaaataaag cattttttat tactgaataa gatagccctg ctgatatatc tccaacactt
     4501 gcaccttctt tttgagcttg ttttactcta gaattcataa atacagtaca tcttgaacct
     4561 aaatctacag gattttgaga ataaatacct tcatgtgcaa attcttctat tgtcatagat
     4621 aaagaacttg caaatgtctc taaaaatgag ccacaacctg atgaacaagc ctcatttaat
     4681 attatattat caattactcc atcttttatt tttaggcact tcatatcttg accaccaata
     4741 tcaagtataa aatctacatc tttattgaaa aatttagcag ctttgtaatg tgctatagtc
     4801 tctatctctc cgttatcgat tttaagagct tttttaataa gaccttctcc atatccagtt
     4861 acagttgaac tcaatatttt aatatttttt ggtaatatat catatacttc atttataact
     4921 ttaatggttg tttttaatgg atttccttca ttgctattat aataagaata tacaagtctt
     4981 ccatcctcat caataagtgc tgctttagtt gtagttgagc ctgcatcaat tcctaaataa
     5041 caattgcttt ttattgaatt tatgtccaca taacttattt tttctttttc atgtcttctt
     5101 gaaaattctt catactcatt tttatcttta aataaaggtt ctaataaatt tacttctcca
     5161 tcttcaatat tctttatact atccaatttt tctattagag attttagttg tatattatct
     5221 tcatctactg ataataatcc tgctccaata gcaatataaa gttgtgcatt ttctgggaag
     5281 attatgtttt catcttttaa atccaatact cttttaaatg cttctctaag ttcagataaa
     5341 aagtacaatg gaccacccaa aaacgcaaca tttccttcta tttttcttcc acaagaaagg
     5401 acactcacag tttgtacgac aactgcattg aaaatactca ttgctatatc agtctgattg
     5461 gctccatcat ttataagagg ttgaatatct gttttagcaa aaacaccaca tcttgaagct
     5521 ataggatata taacattata ccctttagca agttcattta atccactggc atctgtcttt
     5581 aaaagtgatg ccatttggtc tatgaatgct ccagtgccac ctgcacatat tccattcatt
     5641 ctttggtcta ttcctccact tagatatgtt atctttgcat cttcaccccc aagttctatg
     5701 acaacatcag tttcaggatg aaaatactct attgattttg tactagatat aacttcctga
     5761 acaaatttta cacctaattt ttttgatatt ccaattccac ctgaaccagt tattataatg
     5821 gtagtattta tatctcccaa tttttcatat ataccattta gtacttcttt aactgatttt
     5881 tttacatcag aataatgtct tctatattct ttatgaacta tattattact attattaagt
     5941 accacaacct tcaccgttgt agaacctaca tcaattccta tatgaaataa ttcatccatt
     6001 gtacccctcc atatttatat gcttttttaa tttttgtaag ttctatggct ttattttaag
     6061 taagttattt ataatcttta tgactaatat ttttatttta tgattctttt attaatcaaa
     6121 gcttactcta ctaggtaatt cctaattcta accttaaatt ttaaataaga acatatagtt
     6181 agtgtactaa attttgtcat aaatttctac agatttttgt tactttttac ataaatataa
     6241 cttaagtcta atatccactt aatgtttatg tattataaat tatctttagt cagaaaaaaa
     6301 tggttgttga tatgtaaatt cttattacat atcaacaacc atttttttgt atattatttt
     6361 ttatcttagc tgattacgtt tatcctacga gtgtaaatat aaagtgagct gctgctcctg
     6421 cacataaacc acctattgta tgagaaagaa tagctttacc tgccaactgt ctagcattta
     6481 atgcatccat cataccaata tgtgtactta aatatccact ccaacacata cccatagctg
     6541 taaatactgc tatatcatta ggagttatag ctcctgactt tataaactct ggaactagag
     6601 acattgctgc accaacagct cccaatgaag ttatagggaa tgctattgcc tctggtgatg
     6661 taaatccaaa taatggttct aatataaatc caagtttatc tccaataact ggaagtaatt
     6721 ttattccttc atatgcagct cctgtataaa cttcttgacc tgtaacaggg tctgtagatg
     6781 gtccaaaagt taataacatc accaatgtac atactactag gactccagga attatagcca
     6841 ttcccatatc tacccccatt ttaccacctt caagaattgc atctaaagct ctttgaaata
     6901 catttccttc tctgatttct ctaaattcat cctttttctc taatgttcct gttgctttat
     6961 cttcttccgg attgtattta taaaacttct tagtaaatgt caacattatt ctaacactta
     7021 tgacgctacc tataattgca cctaaattac caattaatgc tggaagtaca tattctgtac
     7081 cttgagaaat cataaatgtt gttaagataa gtcccatacc aaaggctgtt cctaagttac
     7141 ataatgctgg aacttggtat tgtttaaaat actgtgtgaa tgttttatct ttagcaaaag
     7201 gtataatagc tggattatct gataaataag ttgttatagc tcctgctata ctagcaccag
     7261 gtagtccata taaaggcttc ataaatacag cgaaaatttt attaattaac gctattactc
     7321 caaactctga aagtagtgcg cttaatgcgc ctgcaagaac tgccatagcc ataattaaga
     7381 aaactgtctc caatagtaaa gcatgtgctg tactcattat tactttgaac atcattcctg
     7441 ctcccataat actaccaaca tacccaaaac caactactag taatattagg aaaatgaagg
     7501 tttccatgct tactgcctta acctgtctcc tgtctgttaa cttctccatt ttgaatcatc
     7561 ccttcttttt ttgttataaa gtttaacaaa accttttctc ttttttaatt atacttataa
     7621 gtattactat ttgcaacttt atttgtcatt tttttatcat ttttaaaatg aaaatcagta
     7681 ttttaataat aaaaattttt cagatttatg caagttttcg acatcttatc tttcatttta
     7741 taaaaagtat gattccttac taaatatata tatacttctc taacgaccta atatgcaaat
     7801 ataagttgta acttgtatct ttttgcatga aagaaaaaag attatgaaaa aatttttcct
     7861 tcaatatttt tcataatctt tttttttagt tttatataat tttttcaaaa aattttttta
     7921 aaatttgctt taagaaatat atattatttt aatttttcag ctacatattt tactaagtca
     7981 acagttcttg aagaatatcc ccattcatta tcataccaag aaactacctt aaccatattg
     8041 tcttctataa ccattgtgga tagagcgtct ataatagatg atcttgaatc tcctttataa
     8101 tctatagaaa caagtggctt gtcacaatat cctaaaacac cttttaattc tccttcagca
     8161 gcatctttta acacagagtt aacttgctca gatgtaacgt tttctttaag ttcgcacact
     8221 aaatcaacaa gtgaaactgt tggtgttggt actctaagtg aaaaaccatt taattttcct
     8281 tctaattgtg gtagtactct tgacacagct tttgctgctc ctgtagtggt aggtattata
     8341 gattcagccg ctgctcttgc tcttctcaaa tctttatggc ttttatctaa tattctttga
     8401 tcgttagtat atgaatgtac tgtagtcatt aaccctttta ctataccaaa tttttcgtcc
     8461 aatactttag caaaaggagc tagacaattt gtagtacaag atgcatttga aattatattg
     8521 tgcttttgat tatcatattc tttctcatta actccgataa ctatagttat atcttcattt
     8581 tttcctggag ctgttataat tacttttttt gctcctgctt taatatgttt ttctgccttt
     8641 tctctttgtg taaataatcc tgttgattct attactatat caacccccag ttctttccat
     8701 ggtaattctt ctgggtcact atatcttaat atttttatct ctttagagtt tactactaga
     8761 gtgtcttcat cctttgcctc tacttcacct ctaaaagttc catagcatga atcatatgta
     8821 aataaatgtg ccaaagattc tgttgttgct cttgcattta tggctacaat ttctatatta
     8881 tctcctaatt cttcttgagc tattcttaat acagctcttc ctattcttcc aaatccgttc
     8941 attcctacct taattttcat tatgaaatgt ctccctccat tactatgttt ttacatgatt
     9001 attgtacact aaataaatca tttgttcaat actttttctt gattaaataa tcaaattcca
     9061 tgttttttta ttatcaaaag acaattagta tatcattttt ctttatttca cccctcattt
     9121 agtatgtact ttttagtgta aataagctga aatcatttat tttactcaat gtcccttggg
     9181 acatattttg ccatcttttt tttgaatttt taaactatac ttatttttaa aaataatata
     9241 tcttcaagaa tctcctctaa tttaaaataa taaaaacaaa tttttattta gttcaaaaaa
     9301 tatatgattt tttaaaattt gccacaatta ttatttatat atcaaaatat aaataataat
     9361 tagcttattg ttaactaaat tatggaaata tacttaattg aaaacttaaa tttatttcct
     9421 tgtgttatca agatttcatg cttaccattt ccaatatcat gctcaccaac ttttgatgtg
     9481 cataaactat ttcaagtaaa aatgcacaac ctatcctttt tgataggaaa aagcagtcaa
     9541 tcataaatat caactgcttt tatgtactga atatcaagtt atagacaagc tggaatttat
     9601 tttgagggtt tttctttctc cttaactaaa ttgctttcaa aagtaacctt tacaactgca
     9661 tgatttattt taattccggt aaagcatata gcatactctt tctctttttg tatattagaa
     9721 agagaaattg cgaatgtatc attatcaacg cttccatgcc atgtattact ccataaagtt
     9781 tcatctgttt tattgtcctt tatttccaca ataccactat ctccatccat tttaaaagaa
     9841 atctctgtgt ctaaaacatc tatatcatta gacacacaaa acaaacgtgc attaacaaat
     9901 gggtctgaag tatcataatt tttatctaag tccatttcaa tggttgtttg tcctgattct
     9961 ttgttgttta aattattata tttactttca ttgctttttt tacaagctgt caagctaacc
    10021 acactaatga tactaattaa aataataact aattttttca tagaatttat ctcctttaca
    10081 atttctaatt tttaacccta caaactataa tattaaacta atctactatt ttacatcaaa
    10141 tttttcaatc attcagcctt taccttttct cattttaatt gcaaatatgc taaataattt
    10201 tggaatttta attattgtag tatcttaaat tggtttataa catattatga aaatttaaga
    10261 gtcaagtaac ttatttaaaa atacacaaaa ttatacttta ttgtataaaa aaagagatta
    10321 taatttatac aagtaaaata aactttatta ttttactctt ctatataacc tcttttctat
    10381 atatctattc aaataattta gctcctaaac tcacaaacat ttgtaaaatc aaaaatgctt
    10441 ttgtgtctat cttatgatta ctttgtaaaa tctcttttgc atcttcttgc gaaattaaaa
    10501 gcgcttcaat ctcttcatct ggttctaaaa attcatctgt tatatcacca tcacatatac
    10561 aataaacaaa ggcaacagat tcatcactca ttccaggaga taaataaact ttatcaatac
    10621 ttttattttt tttaatttct aaaagagtaa gtcctgtttc ttctctaagt tctctttcaa
    10681 cagaaatatc tatagactct ccttcatcaa caagacctgc tggtaactcg tatatatatc
    10741 cattaatagg tactctaaat tgtcttatta aaattaattt tttactactt ttatgaagcc
    10801 caactattac aactgcatct acactatctt cttcattctt caaataaata ttttttaatt
    10861 gctcactact ttttcttgat gctaccatcc aaaccttatc ttcatctaat ttgtttttat
    10921 actctatttc aaaaagtcct ataaacttat tttcaatcat tggatttaca ttctttatta
    10981 tagttttttt caaatttact acgacctttc ttttttaata cctttttaac atcattaagt
    11041 aaatataata tcttaacaag tattcaacaa cttattatat ttccatttca ggattatctt
    11101 tatctacaaa acctactttg atttcacaat taaatatttc ctttaacaat tttgctaatt
    11161 cttctcttgc actcattacc ttatctaaat tagcatcagt aaaaccatct ataggaaaaa
    11221 atatattttt tgagctatta gtaatttttc cttcctctcc ttgtctccat atccatcttc
    11281 tagtttttac catgtctcca actgaataaa ttaattctcc ttcttccatt aattcaacat
    11341 cagtttctcc aaatggtaca aatttatccc cttttttaga aaatctaaca tatacatcat
    11401 catttctaaa atccatatca tgagcaccca ttggaagtaa atattttaaa gaaatagagt
    11461 ttcctaaatc tacaatagga tttatatgag gaagtttctt attcttagca acccttgttg
    11521 tcattgcttc tatcgaactc ataaatttat ttgggttcat gcctagactt ctaaatgcct
    11581 ctctatatgg tataatttct tctgattcct ttactttttt atctttgaag taatcttcta
    11641 ctctatctat acttatatct aaaaggttgc ttattctttc tatttcttta gtattatcta
    11701 ttccttttgc aactactaca ccaaagcata cattttctaa tttatcaaaa acttcttttt
    11761 ctactataaa tttcattttg ctttatcctc ctgttatttt acattatttt ctaaatagat
    11821 tttatcactt tgtgtcttat aaatataccc cctatttgct aattgtttaa tttttttaaa
    11881 ttttccaatt aatattatat ttattcttat cagatatttt atatgtacta attacaaatt
    11941 taactttcca aaacaaatat cttgttttat aatgtataat atatttttat ggaggcaaga
    12001 ctgtgaaaaa ttttattcta atatatttta tagtgattaa ttctattgct ttcttttcaa
    12061 tgtacataga taagaaaaga gcaattagaa acgaatggag aataaaagaa gctactctta
    12121 tgtctatagc agtaattgga ggtagtatag gttctatgat tggaatgtat tcttttagac
    12181 ataaaactaa acacattaaa tttacatttg gtataccatt tatactattt ttacaatttt
    12241 tattagtata tttttatatt ttaaagtaat tgaaatataa aaccttattc ttttcaataa
    12301 attaaggtgt ataaaattaa aaactttata cacctttttc tagcaattat taaaaagata
    12361 tttaattaaa attaatgacc cttatgttta gcttttctct tttcttgttt caaattaaat
    12421 attctttctt tttcttcttc ttttctattt ttagctaggt tttttctatc ctgttttctt
    12481 tcttcatatt gaagcttaag agcattttga gccttagtcc ctatagtttc tttttcttgt
    12541 tcctttttta cttttctctg cattcttttg tatccaatgt tgtctttggt cacatttttt
    12601 tctaatttaa tgctaccaaa atctaatgag aaaaatcttt caagtataaa ttcatacact
    12661 tctacttctt taggttctgc tccaaaaact accctacatg cttcatattt tttaccatct
    12721 tgtctttcaa atatacctac ccaaaatggt tcttcaaata agactgttaa ttttccacta
    12781 attttgtcca tcgctagttc ctccttaaat tactttctaa agaacggaca accaaggagg
    12841 caggttactg acagctattg ctgcgtctgg actaccaacc agactgtgtt tttatcttta
    12901 tagattaaat attatctttt ttaatcatgc ttgtcaatct tagtatcaac atctatcata
    12961 ttgatttaag tgaaatcaag ctttttaact ctactttatt attgttaaca ctaagaaaat
    13021 taattatttt tcataataaa ttagttatta tacttattta aatatcaatc tttattgttc
    13081 cctgcataaa caagactgct tcaccagaaa tttttacagt attatccttc aacacttcac
    13141 actgaattat accaccacga ttagaaagtt gagcagctac cattttgtgt tttcccaact
    13201 tttcagacca gtatggaata aaattacaat gtgatgaacc agtaacaggg tcttctgaat
    13261 ctaattctgg acaaaaatat cttgatacaa aatctgtatt acttccttgt ggtgtgatta
    13321 ttatacctaa ccagtctgtc aatttacgca attgtgcgta attaggttta taatttataa
    13381 cttcttgttc actgtttagc aataagatta aatctcttga agaatagaca gcacttggta
    13441 cacagcctat caaattagct tgttatggtg ataactctat ttcaataggc attatttctg
    13501 gaaatatcat ttcatataga tttccatttc gtgtaacttc aagctttcca ctttgagtaa
    13561 agaaatctat cctttttaca ttaacatcaa taaaatttga tattacataa gctgctgcta
    13621 aagttgcatg accacataaa tcaatttctg ctttaggagt aaaccatcgt aattcataat
    13681 ttcctttacc tttaactaca aatgctgttt ctggtaaatt gttttcttct gctattttct
    13741 gcattaactc taaaggtatt attctgtcaa gaacacaaac tccagcagga tttcctttaa
    13801 atagtttagt tgcaaatgaa tcaactatgt agtattccat tatttgacct ctctttccaa
    13861 cataaaatat acatcaattt acattcaata aaactttaaa tttcttacat atatttttta
    13921 atattgctct gctcaacaac ttttttacct aacaatgtaa ttgctttttt tgcacaatta
    13981 tttacacaac gataacacat tgtacattgc ccatttgcaa caatcataca atttttaatt
    14041 actagattct tcattggaca taaattaaca catttaccac atacaataca ttcctcagta
    14101 ttaattttta gtttatctgt atacatattt gttttattga taaaatacaa tcgttgtcca
    14161 aataacccta caatatgact caaaaaacct aacccttctt ttggtggtga cccatgtttc
    14221 agttttttag cagctttagt tactttcttt tctgctttta ttacaagctt tttattatac
    14281 tctaaagaat gttttaatac cttttcatct cctatactat ctggcatctt cacatgtaat
    14341 ccaccaacag ttatagcacc gtattttttt aatattctag ctaacacacc agagccatct
    14401 ccactaaata gagccatagt tgcaataata aaaacacgtt taccattcca caaagttgaa
    14461 tgatttatta taaaatcttt caatattttt ggcacattac tatattgcac aggataacta
    14521 aaaataatat tctcatgatt attgatgtgc tgtaaaagtt cattatcttc aatagaataa
    14581 gaatttgctg ttatatcata ttcttgtaaa aatttttcta cacagtatct tgaatttcct
    14641 gttccactaa aatatattcc tatcatagta cttctcctaa tagctaagat tatatagctc
    14701 tattcttaaa attctgactt tttatattat ctaaattaag tagtttattt aaataatata
    14761 aacttaattc aacaagttac ctactgaacc tcccatgact aaagtcatag ggttcctgct
    14821 tgatagaatt ttacataatc aatttactaa atacttgtct aattcttttt ttatcatttt
    14881 aaatatacat tttaataata ttagtaagct atacttcctt aagcatatat tttccctaaa
    14941 gtaaaactat tgatgattat tccattatat aacctaatta taaatttagt aagttcatca
    15001 catactatac ccttaattct caattataaa gttccctaaa aaatatttga aaatttaact
    15061 atgaaaagat ttcctattgc ctaatatgta taagtaagga gtactatata gtactcctta
    15121 tattgtactc ttataacatt gataccttaa tattatttta atcttaatca ttattgattt
    15181 gatttaaaat tataaatagg ttccaatatt ttaattatgt caactatatc tcttatagtt
    15241 ttaataatct catcaattgg tttatgtgct tgttgcactt tatcaatagt attaattaaa
    15301 tccatttaca catctagcac ctgtatggca atttagcatt atccttatct tatactttgt
    15361 tgtaaattct tgattacata attctattat ctaacttata gctgtgcaat ctacattatc
    15421 tgtaaatatc tttgctttat atattttccc tgaacctcta acattaatat aacagccttt
    15481 ttgctataca cttttaacta tatacttaat tttaataaca ttacttgttt ataaaaatat
    15541 actatagata aatttcacat aaaaataata attcaagtat tgatttttat ataagaaaat
    15601 ttgtaatgta ctaaaaataa cataaaagtt tccatttttc aatatgtaag cttttcttat
    15661 gttattttat tgttaaaggt ttcaagtttt tatttattca ttttaaaaaa attattattt
    15721 ttgtaaaact aatcccatag tcatactatc gtcagaatat gcttcaccct taacattact
    15781 aaaaaactca acagacttaa atccaatatt ttcaacttct aataagaaac tttcttttga
    15841 aaaatagtga ttccaaacat aaaatacatt ttcatctttt tcggttatta caagagtttg
    15901 ttctaatgta ttataatcat tgtatttaca attatcttgt agacacatat atttttcatt
    15961 actccaaaat cctccatctt catttatttc ccaatattta atttcctcaa aattattgta
    16021 tttatttatt gtaaatacat ctaatattaa ttttcctcca ggttttaaac tatcataaat
    16081 tttttccatt aataaccttc tattttcagt tgacaaagct ccataatcac aataaattaa
    16141 agtagccaaa tcaaattcat cattatagtt catatttaaa taactttgaa acaagtaatt
    16201 tatgtttaaa ttagtctcct tatttctatt ttgtgcatag ttaatagaac gttttgagaa
    16261 atcaattcca gttaccttat aacctttttg tgcaaatttc tctgcatata aacctggacc
    16321 acaacctaaa tcaagtaaat taggataatt aactggattt gcaactgtaa ctatccaatt
    16381 tacagaatcc tcgataaagt taaaatttct acttgctcct tcaaaattag tgtctaaatg
    16441 tgcttttaat aattgttttg aaatatactc atcatcccaa aaattaattt cactttgttt
    16501 ataaagctct ggtctttcta aatacataca taatttgttt aacaaaacat accactcctt
    16561 aaaatttatt tttaaaataa aaaaaccaca gagtttactc tgtggtttaa cttcaaaaag
    16621 atgagtttaa ataagagtaa aatagaacca aatattttta agcacaacaa taaaactata
    16681 gctttacttt gtttgcttat taaatttgat tttattcact cttttccagc aatacttata
    16741 tcaacgattt tatgatatca aatatcaccg aacaacatca tcctttcata tttttatttt
    16801 atgaatcaat tatatattta aattttatat ttgtcaataa attttaattt taataaattt
    16861 taatcatcat agtaaaaaaa aattttttat ataaaactaa tcaaaaaaag atatctgtcc
    16921 atctcctgag ttttttaact tttccttcat tattttagag taactgcttg atagagaata
    16981 tttatatcta agttcattaa ccattttata caattgattt ttatactccc tattaagtga
    17041 acctgcacta tatagtattg ataatttatc aaataattca ggaaactctt ttttaacaaa
    17101 gtcaaaaaaa acacctctag ttatacccct taagtataaa gtcccaggta aaacataatg
    17161 tacattacat tcttttgcat gtctaaatag cgaatttata ttatcaaaat catctgtaat
    17221 atatggtata attggcataa tatgtagacc aactgatgcg ttagtttttc taaattcttt
    17281 taacatttta aatcgtctca ttgaatccac accatttggc tcaataagcc tttgtgtatc
    17341 ttcatttact gtggtaattg tagatgctac attgatatat gtaatcctag atagcttatc
    17401 aattaaatcg taatctctta aaattaaatc tgactttgta gaaataatag ctggtgtctt
    17461 gtattttatc agcaggttta atatttctgg cataatttta tagtcagctt ctattggttg
    17521 ataactatct gtaactcctc ctatattgat tacttctttc ttccatttac tgcttttaag
    17581 ttgtctctca agtttttcta ctatatttgt ttttacataa atctcttcaa aataattatt
    17641 ggagttaatg tacttatgag agtaaatagc ataacaatat ttacatccat gctcacatcc
    17701 acgataaata tttaaatccc atttatatgg catactcctt tttaatttat tacaagctat
    17761 ttcacaattt attggaatat attttatttt attatacaaa taatcacctc tttatataca
    17821 ataatactat aatgttaatc tatagtaatt aaaacatgat atttccagac cacaattcat
    17881 aatctttact atatttacaa agtaatatat tccattcatt tattggtatt cctccacatg
    17941 ttgctttttc ttctaaatta tataagcatt gctttaaata cattttatta tgccaatcat
    18001 catataaatt ttctaaattt aaactatgtt cattagatat atcatttgca aaatttctat
    18061 ttttccattt taatatactt gtatatttaa gttcattagg tttataattt ctttttaaca
    18121 tttcatcatg tacaattttt gtataaaatt taaattcatc tatactataa ttcaatactt
    18181 tattcactaa tagatggttt ggagtacctt ttttatctat agaacctttt atagcaagta
    18241 gctctctcca ttgacttact aattgatttt taggtaatac atcaattaaa tccttatgcc
    18301 ataatctcat aatttaactc cttactataa aatattttat aaattcaata accaatctat
    18361 cttacaattt tacatgttca ataattttat ctgtaaaaac tataccattt aaatggtcta
    18421 tctcatgaca aagacatttt gccataaaac ctgaaccttt aatttctatt ttctcaccgt
    18481 attcgtttag agcttgcact gtaacttttt ttggtctttt taatttaccc catacttctg
    18541 gaaaacttaa acatccttca acaacaattt gttccccttc ttgtttaata atttcaggat
    18601 taacaagctt aataagtcct tctcctaaat ctataacaac taatcgcttt aatactccta
    18661 cttggcatgc agctaatcct cctccatttg gagtattgta catggtctct gccatgtcat
    18721 ttaatatttc tcttatctta ttatctacgt tttctacata cttactcttt tttcttaaaa
    18781 tttcatcatc aaatgttctt atctttctta tagccataat attctcccct acttatctgt
    18841 gctcttgatt ttctttcact ttcttcaaat aaaaatacac tttcaattgg ttcattaaaa
    18901 attcttgaaa tatcatatgc caaccatatt gaaggttcat ttttaccagt ttctatagca
    18961 tttatagcct gtctagatgt tccaactaat cttcctagtt gttcttgtgt caatcctaat
    19021 ttttctctta atatctttat tttatttttc atatttgctc cattcatgta aggttaactt
    19081 tacatcttaa atgtaacatt tttcaacatt actgtcaagt caaccttaca tttatatatt
    19141 aaattttctt atttaactat ttttttacta aactttttaa ataataataa aatccatatt
    19201 aatgatataa caattgaaat aaacatatag atataattat cttccttaaa tgccttcgat
    19261 atccaaaaag ttggtaagaa gaacagaata tattgtgctt tatttaaaat aaaaaatggt
    19321 gctaaaatac ccatcataaa aacacctgag aatttcgtaa ttaccattcc ttctagttta
    19381 tttgatgata aagaaatcac tagtattgat ataataactc cttgtaataa aactaataaa
    19441 gaaattgata agtttaacaa gaaagaaatt ttagttagag aaaaaaagat aagtagaatc
    19501 atagtaataa taaatgctaa ggtacctggt attccaaatc gtgaaataag ataaccagtt
    19561 ttaccaagcg gagttactat taaatatctt gaaatgctgt catcaatttc tcccaatatg
    19621 accattgtag aagcaaaaaa gaacataaaa ggtgtaataa aagctaaaag taaatcaaac
    19681 atcaagaaat acggctctaa atttaattga taataaaact tgttaagtga tatattctgt
    19741 attaatggta ttccaaattt tataaatact ccacataata ttggagaaac acatactata
    19801 aaaagcatag catctctctt aatttgttca aactcttgtt taaacgcatt tataatcttt
    19861 atcatagttt tactccacct acctttttcc aactgctaca aataaaataa taagttataa
    19921 aataaattat tattataatt gatattaaaa tattaatcat tataaaattt ttatctccac
    19981 taataagaga aatacatata ttaaaagggt acaaatttgc atatgatttt gtgtcaagaa
    20041 gtacattgag tattggtgga ataaagcata taatctcaat aggaatactt aaaacaataa
    20101 actgattcaa actagatgct ttacttgcaa gtatcaatcc aagcaaagaa aatataatag
    20161 aagaaaaaaa ggtacctata gttgatatta taatattatc taaatttaat gtaatagcaa
    20221 taaacatagc tacaatacta gatattactc ctaatgaaat tacctttgat aaaatatact
    20281 cagatacttt tactggagat acagctaagg aatttaaaac cctttgactt ttctcaagaa
    20341 gtacaatagc tcccataaaa aacaatccca ttgctgctgg gtcagaataa atcataatta
    20401 tagctatttt ttctctcata aaagttggaa atacattgat aatacaaata tatataatac
    20461 ttaaaaaaag atataaaaaa taaaaaccat acttaaattg aaactttata tctcctaaaa
    20521 taagattttt tagcctcatt gtaaattcct ccctgttatc tcaataaaaa tatcatttaa
    20581 tgttggttca ctactatgta ttgataaaag tttatttttc ttaataagca tattcaaatt
    20641 tttatcatta gcagtattgt tcagaaaaca ttcagaagtt ttttctccat tatcaaagta
    20701 agtatatcta actttaatag ctccttttga cataataaga ttatgtggtg tatctaaagc
    20761 agatatttta ccatttacaa tgaacgctac tctgtcacaa agctccgttg catctaacat
    20821 gttatgagtt gttaaaataa tagttttacc tttagatttt tctgataaaa ttatatcttt
    20881 cataaccttg ctatttgacg ggtcaagacc acttgttggt tcatctaaaa ataaaatatc
    20941 tggattatgt aataaagctt ttataaaatt taatcgagat ttcatacctt ttgaatactc
    21001 agataccctt ttatttgctt cattttctag tcctactgat tttaataact catcaataga
    21061 tagtagtttt ttagaatata gagacccaaa atattttaaa ttttctattg cagtcaactt
    21121 ttcatataaa ctaggaaatt caaaatcaac tcctatattt tcataaaatt tatttgagtg
    21181 tcttttactc tctactccat ttacaatgac acttcctcca taattagtta tcattccaat
    21241 tagaatcttt tgtaatgtag attttcctgc acctgatggt cctaaaaatc ctagtatttc
    21301 ccctttttct acttcaaagt taatattttg taaaaaattt ctatctgtat aactaaatga
    21361 taaatcatca actttaatca ttcaatatca ccctttcaaa tttttgacta ttttgactat
    21421 atggtcaata aaatataatt tttttgtttg ttaataattt ttaataaaag ttattagcaa
    21481 acaaaattta aattctattc cataagttgt ataactaatc cgtttatcat taatcttaaa
    21541 gcatcttcaa aacaattact tccaacctcg cgcttatata gcatggtaag aaaaactact
    21601 ctaaatgctc cactaaagct ttctatgtct ttattttttg catttggtat gcaagatata
    21661 atctctccaa tttcaaagtc atcatgtcta aagtgttctt caattataga atctggtaac
    21721 tttctcacca atatctcaaa ttctccattt atcatgagat ttaaaattga agtactatca
    21781 accatcctat agaatttcat aaatatatct gtcaattgtt cacatgtaat actttcatct
    21841 actctattta tctcatataa gatttctttt tgtatagagt catgtatatc tctcaatacc
    21901 tcaaaaaaga gcaattcttt tgattgaaaa aatagataaa aagttccttt tggtattttt
    21961 acccgtttta caagctcatc aacagtagtt tttctaatac catattgctc catacacaat
    22021 cttgcttctt ctttaagtct ttttattata tactctcttt ctttatcaga atacgattta
    22081 ggcatctttt tcctccagtt tgactaaaat aatttatata gtcaatataa tactttttct
    22141 atctaatgtc aatagtatta ttagtatatt taaaaataag cttaaaaact ttcgaaaatt
    22201 ctgaatttta tatagcattt tttattatct agtgttataa ttaagatata aataatatat
    22261 tattctaatt ttcatatcta tttgcttatt ttttaactta gtttctaata gaagtactca
    22321 tatttatctt tttaaaagtt tcaactttaa taaaaaataa aggagttgat tttatggcta
    22381 ctactacaaa aaaccgtttt tacgattatg ttcaagaaaa cttaaataaa tatgttgaag
    22441 tacattctta ctttagcagt tgtcctctat atggtgaaat tatagaagca gatagcttat
    22501 caattacact ttgctcaaga tatatagatg aatctaatcc aactttaaat gaaacttaca
    22561 cattatattt accattaagt tcaataatat ctattagaac act
//
LOCUS       DAELXX010000067        22201 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.067, whole genome shotgun sequence.
ACCESSION   DAELXX010000067 DAELXX010000000
VERSION     DAELXX010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22201
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.067"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            159..335
                     /locus_tag="KPH29_002331"
     CDS             159..335
                     /locus_tag="KPH29_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467919.1"
                     /translation="MEINVIEIFPKDKAKLNKIEMDKASWFVNIISKKYPKEALNEAF
                     SILEKELNISKANT"
     gene            509..1639
                     /locus_tag="KPH29_002332"
     CDS             509..1639
                     /locus_tag="KPH29_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG1467920.1"
                     /translation="MNNFEIKTRIKFGEGSLKVLQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKSIKLVFKYLIKAYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSDTAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILNNIK"
     gene            1880..2230
                     /locus_tag="KPH29_002333"
     CDS             1880..2230
                     /locus_tag="KPH29_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBG1467921.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKRD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            2238..2687
                     /locus_tag="KPH29_002334"
     CDS             2238..2687
                     /locus_tag="KPH29_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HBG1467922.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELKYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDDIGLEKLFKYLEKSKNIDEV"
     gene            2789..3364
                     /locus_tag="KPH29_002335"
     CDS             2789..3364
                     /locus_tag="KPH29_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBG1467923.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENLAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            3357..4766
                     /locus_tag="KPH29_002336"
     CDS             3357..4766
                     /locus_tag="KPH29_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HBG1467924.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     VLSKFDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGKLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFENIL
                     MDENYDETNEVSIGNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGGYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFS
                     DREHGTIQILINKDNDYRNIAIIDDGIGFDSNNINKNSLGTYIIDSYITQMLKGNIER
                     KSNENGTEVLITIPTQINI"
     gene            4950..6383
                     /gene="eutA"
                     /locus_tag="KPH29_002337"
     CDS             4950..6383
                     /gene="eutA"
                     /locus_tag="KPH29_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HBG1467925.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKEDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVTDTGCLDIGGRLVKIDKISKKITYISPKIDKIIKDNNLNLQVGTMATVENT
                     KPIVDIMVDLLMESIGLKKQTPLFDFIITNKSISIDENIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQTIKNCEYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSVDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYEKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            6401..7765
                     /locus_tag="KPH29_002338"
     CDS             6401..7765
                     /locus_tag="KPH29_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HBG1467926.1"
                     /translation="MILNTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWLLD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIKVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKHG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFYKDGKLTELAGDAS
                     VLLG"
     gene            7778..8659
                     /gene="eutC"
                     /locus_tag="KPH29_002339"
     CDS             7778..8659
                     /gene="eutC"
                     /locus_tag="KPH29_002339"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014952556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HBG1467927.1"
                     /translation="MNEKDLKALVEQLVGQMVGELDTNVVSETVKKTTEVVVDNNAFI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRAGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKENNMFAVETLCKDKDEYLTRPDLGRKFSQETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            8679..9332
                     /gene="eutL"
                     /locus_tag="KPH29_002340"
     CDS             8679..9332
                     /gene="eutL"
                     /locus_tag="KPH29_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HBG1467928.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEIAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            9343..10044
                     /locus_tag="KPH29_002341"
     CDS             9343..10044
                     /locus_tag="KPH29_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBG1467929.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKSANVNIVRIDKVGAGIVTLT
                     ICGDVGAVKAALEAGEIAASRVGTLRTSHIIPRIHNEVTNALFKTKEPKVCEVSEDKE
                     KNFEITLEITDNVSKNLSEKENVILDVNENSGDTENLYSDIDKTNKKIETVDDSLTEA
                     NEEKIENTNDKEVLEQVKIENSDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLKK
                     EELINLVKKLSRRDR"
     gene            10047..11516
                     /locus_tag="KPH29_002342"
     CDS             10047..11516
                     /locus_tag="KPH29_002342"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HBG1467930.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKAVV
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIIGEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISE
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNIPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALN
                     MPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSMDKEELINLLVQKLVKELA"
     gene            11612..11899
                     /gene="eutM"
                     /locus_tag="KPH29_002343"
     CDS             11612..11899
                     /gene="eutM"
                     /locus_tag="KPH29_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HBG1467931.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGAAAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            12026..12787
                     /locus_tag="KPH29_002344"
     CDS             12026..12787
                     /locus_tag="KPH29_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HBG1467932.1"
                     /translation="MGVLTENEVKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEVVKEVVKEKKYKYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKVQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLDEKLEDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            12800..13429
                     /locus_tag="KPH29_002345"
     CDS             12800..13429
                     /locus_tag="KPH29_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBG1467933.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGE
                     GYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGSTLGL
                     KAPIKQSGDLEGTLSIKISTQYGEVELDKGLMVAKRHIHMTPKDAEKFNVCDKEIVEA
                     KVMGQRPLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKA"
     gene            13471..14196
                     /locus_tag="KPH29_002346"
     CDS             13471..14196
                     /locus_tag="KPH29_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02536 family ethanolamine utilization
                     protein"
                     /protein_id="HBG1467934.1"
                     /translation="MNYDNLVDLITEEIYKKINSNEIKISNKPKAVIVFEQDNNKFNL
                     LKDEFEILEFDKSIRECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMKGKKVYV
                     LDEGIEYKKYKDTAPKALYKKFMSFEDEICKFGVEIIKDLNVITKNKLNIKKELESTR
                     GSKKEDTKVLDKDVLNLTSVESVKIDNEFSLDLRNKKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKL"
     gene            14209..14481
                     /locus_tag="KPH29_002347"
     CDS             14209..14481
                     /locus_tag="KPH29_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HBG1467935.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKDTYKEGFMVAA
                     DNAGAGNGDLVLISKGSSARESIEKSHVPVDATIVGVVDSFEVFDE"
     gene            14474..15025
                     /locus_tag="KPH29_002348"
     CDS             14474..15025
                     /locus_tag="KPH29_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBG1467936.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     IVGGETSYINECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQKLDSIVSIGVVE
                     SSKVCAGIKMLDKTLKSGDLVLVKLQLSFAIGGKLVYVVAGDLSSLEYALKESENVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            15037..16128
                     /gene="eutH"
                     /locus_tag="KPH29_002349"
     CDS             15037..16128
                     /gene="eutH"
                     /locus_tag="KPH29_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBG1467937.1"
                     /translation="MSINEIIIYVMVVFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NSGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEVMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGLAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLRKENV"
     gene            16130..16603
                     /locus_tag="KPH29_002350"
     CDS             16130..16603
                     /locus_tag="KPH29_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF861 domain-containing protein"
                     /protein_id="HBG1467938.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
     gene            complement(16723..16824)
                     /locus_tag="KPH29_002351"
                     /pseudo
     CDS             complement(16723..16824)
                     /locus_tag="KPH29_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            17119..17640
                     /locus_tag="KPH29_002352"
     CDS             17119..17640
                     /locus_tag="KPH29_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG1467939.1"
                     /translation="MEEIVKKEQASMNDIFLYLKAKSNSLYKFVILYNEFINKPKDYG
                     TGELINMVEVHMLTAIEENPGISPTQLAKIWNRTKGAISQTISKLEKKGYITKEKKEG
                     NAKTILLYPTTSGVELSKAHKLYDTIDVTRTFEYLIKECTLEEIDSFYKVIDLFINLL
                     EESPQENNDSEKV"
     gene            18019..19194
                     /locus_tag="KPH29_002353"
     CDS             18019..19194
                     /locus_tag="KPH29_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG1467940.1"
                     /translation="MNLKQLSEKYEEYVIQMRREFHKYPELSFKEIRTTQRIREELQK
                     IGVPFVSIEPNIVIATIQGKNEGKTIAIRGDIDALPMQEDSDVEYKSEYDGIMHSCGH
                     DAHGAMLLGLAHMLNDIRKDLKGRVLLVFQAAEEVGGGHKEIIQYFKENGGPDRLIAT
                     HVWSDIEAGKISVEPGPRMAGGSGWRIDITGRGGHGSRPDQSIDPIKPACDIVLKVSS
                     IPVNHISVLEQCVVHACAIHGGTTIGNVIPNNAEVIGGYRYFTEESGKKVFDIIKQMS
                     NGVGQAYGVDVKVTHTGGIGPVINDEEAVKMAKEIVSNIDGLELDSYEPICASDCYGE
                     ILKEYSGFYCYLGVGNKEKGIIYAQHHPKYNIDEDTLKLGCEFMAKYAVEFLNKNNN"
     gene            19229..20767
                     /locus_tag="KPH29_002354"
     CDS             19229..20767
                     /locus_tag="KPH29_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBG1467941.1"
                     /translation="MQGALKENKKKSGGGFLGWIERVGNKMPHPLALFTYITLIVVAL
                     SAIASFLGFSATSPATGKLIKVMNLISAKGLVLFMQEFVKNFQNFPVLGVVLVMAVAT
                     GVCEKTGFFSTAIKMSLSKVKGNAVVFIIAFIGVFANQAGDAAFVLVPTLAGAIFYGL
                     NRNPLAGIFCGFASVGGGFATAVIPGGWDVTLTPITVSAAQTIQPAFDMTLLASYYAL
                     FVSAFIVATVSTIVTVKFIEPKLGKYTGKPEGIDDNQGFEVTKEQAKAAKLAGFTVLA
                     YVALLIALCIPANSFLRSPEGSLIFGAPLMSCLQFFIVILFFLPGIVYGMRVGKIKTV
                     KDLNDILCQSMAAMAPFIVLAIVIGQFLTLFSVSNLAQVLAIKGGALLNSLPLPPQVI
                     ILLFLVLVAFINIFVISGSTKWMIFGPVFVPMLMQLNIHPAFTQFVYRLGDSVTNHLS
                     PLNAFFIILLATVQKYDKNAGMGTIFSAMIPYTIGYFVVLAVLVVVWMTIGIPVGIGG
                     QVWL"
     gene            20799..22001
                     /locus_tag="KPH29_002355"
     CDS             20799..22001
                     /locus_tag="KPH29_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG1467942.1"
                     /translation="METTEIRYNFDEVIDRSGTNCLKYDLKSQFMPESPEDALPLWVA
                     DMDFACAPEIINAMHERIDRKIFGYSSQQTDDYYEAVCGWFKRRFDWKIEKENIFFSP
                     GVVPALGFFIQILTQKGDGIIIEKPVYYPFEDKITNNERKVVNSPLKFEDGHYSMDYD
                     GFEELAKDENNKVFILCSPHNPVGRVWKKEELRKIVDICKKYDLWIISDEIHCDIIRK
                     SEKHIPLEVACPDYKDRIITCTAPSKSFNLAGMQLSNIVINNKELQEKWMLETDSKLA
                     IFLPNPFAIVATKVAYNDCEDWMNQVNEYIDGNIKYVEDFVNEYMPKVKIIKSQGTYL
                     IWLDFRGYGLDAKQLEYVMLNKAKVLLDEGYIFGEEGAGFERINVASPRTIIEECMNR
                     IKCAFEGL"
BASE COUNT         8999 a         2491 c         4128 g         6583 t
ORIGIN      
        1 ataaattttg atatttaatt gtatcaggac aaatctctaa tttcatatat tattaatata
       61 aaatcatagg ttagtgtgaa aaagttgaaa tttaaatatt ttaaaagata attttttgga
      121 actaataggt ataacaaaaa tttatagggg gattgcatat ggaaatcaat gttatagaaa
      181 ttttccctaa agataaagct aaacttaata aaatagaaat ggataaagct agttggtttg
      241 taaatataat aagtaaaaaa tatcctaaag aagctttaaa tgaagcattt agtattttag
      301 aaaaagaatt aaatataagt aaagctaata cataagcttt gattattgat tttatatact
      361 attggtttta tacgctatga cttgacatta tattaatcaa atagtatatt tttaaatata
      421 gcacaatgaa gtgttaaaag agcaatggag ctccatagat aaagtctatg gagctttttt
      481 gcgtatttaa cttaaggagg atagagcttt gaataacttt gagattaaaa ctagaataaa
      541 atttggagaa ggttcactaa aagttttgca agaattaaaa aacaaaaatg tattaataat
      601 aacagaccct tttatggtga aatcaaaaac aatagataaa ataacatcta atttaataaa
      661 ctcaacatat caagtttttg aagaaatagt accagaccca ccaatagaag tagtagtagc
      721 aggaatagaa gcatttaaat ctataaatcc agacgtaata atagcattag gtggtggttc
      781 tgcaatagat gcagcaaaat ctataatgga cttttctaaa aaaatattaa aaattaacga
      841 agtagagttt atagcaatac caacaacaag tggaacaggt tcagaagtaa catcatttgc
      901 tgtaataaca gatagtcaaa aaaaatgtaa gtatccatta gtgagtgatg atttacttcc
      961 agatatggca atactagacc cagagcttgt aaaaactgta ccaaatttta taacagcaga
     1021 tacagggatg gatgtattga cacatgctat agaagcttac gtatctacaa attctaatga
     1081 cttttcagat gcactagctg aaaaatctat aaagttagta tttaaatact taataaaagc
     1141 atataaagat ggaaatgaca tagaagctcg agaaaaaatg cacaatgcat cttgtttagc
     1201 aggtatggca tttaaccaag catcacttgg cataaatcat tctattgcac atgtattagg
     1261 tggaaaattc catataccac atggaagaac aaattctata ttacttcctc atgtaataga
     1321 atataattca tcaatagtag gatatactaa cacaacttac tctgatacag ctaaaaaata
     1381 tgcacaaata gcagaattag taggacttgg gagtgctaat ataagaatat ctgttagaaa
     1441 tttagtaaat gaaataaaga aactacaaaa agaaatgaat atgccaacta aattgacaca
     1501 atgcaaatta agtttagaag atataactaa tgaagaaaga gaaatagcaa aacttgctat
     1561 aaaagatact tgtacattaa ctaatcctaa ggtaccaact gaagatgata taataaacat
     1621 attaaataac attaaataac tacattttat tacatgtaaa tataatatta tacaaatagg
     1681 gaaaatgttt ttaaaaatat aaattatata ttgacaaata taaaacataa aactataatg
     1741 aaagtatcac aaaggcgtga tactttagta gaaagcaatg gagcttctta ggaatattaa
     1801 tattattctt atggagctcc ttttatttta agcaaatagc tttttaataa actatattca
     1861 aagaaagcag ggaataaatt tgactgaaga atctaagcaa agagttattc aagaatatgt
     1921 tcctggaaaa caggttacat tagcacatat catagctaac cctaatgaag acatatataa
     1981 gaagttagga ttagtacttg ataaaagaga tgcaatagga atacttacaa taacaccaag
     2041 tgaagcgtca ataatagcag cagatgttgc tacaaaggct tcaaatgtat ctttaggttt
     2101 tatagataga tttagtggtt cagtagtaat atcaggtgat gtaagttctg tagaatcagc
     2161 gttaaatgat gttttagaag tcttaggaaa tatgttaaac ttctcatcta caaaaatcac
     2221 aaggacataa taatacaatg aagaatataa tatttatggg aaagacaggt agtggtaaaa
     2281 ctaccttatg tcaaaaacta gaccagttag aattaaagta taaaaaaact caatcagtgg
     2341 aactttataa taatgcaata gatactcctg gtgagtatat ggaaaataga aacctatata
     2401 atgccttaat aacaacatca gcagatgcta aaattatagc aatagtgtat gatgccacac
     2461 aagaagaaaa ttatatagca ccaggttttg ccagtatgtt ttgcaaagac gtaataggaa
     2521 ttataactaa aataaataaa gctactgaaa acgaagtcaa tatagcttat gaaagactat
     2581 cactagcagg agcaagtcaa atctttaaaa tagacacagt tgatgatatt ggacttgaaa
     2641 aattatttaa atatctagaa aaatctaaaa atatagatga agtttaaggg gaaatatttt
     2701 tttattcaaa aatattatta aagtatgata aaataaaaaa atgtataaac tattttaaga
     2761 agatttaatc tttaagcgag gtaggaacat ggggaaaaaa atactattgg ttgatgatga
     2821 accactgtta aggatggata caaaagatat attagaaagt catggctatg aaataatagg
     2881 cgaagcaagt gatggatttg aagctgttga aatgtgtaaa aaacacaaac ctgatttagt
     2941 tataatggat atagagatgc caatacttga tggtataaag gcaggcaaaa taatatgtaa
     3001 agaaaatcta gctggtggag tacttttatt aacttcattt gaagataaag aatacgtaga
     3061 aaaggctaaa agtataggtg cttatggata tttagtaaaa acagcaagtg aaaaatcact
     3121 tatcccaacc atagaaatgt gtttaaataa ggtaaatgaa tttgaaaaaa tgaaaaaaga
     3181 tttagaaaaa gtaaattcta aattgaatga aagaaaatta gtagaaagag caaaaggtat
     3241 acttataaga gagtttaaga tttctgagga tgaagcatac actagaataa gaaaattaag
     3301 tatggataaa agaactacaa tgatggaaat tgccaaaatg attataatag gatacgatga
     3361 ctaatacgat aaaaaaaata tcaactgcac aaacatactt aaataataac gaaattgaaa
     3421 aaattataga agtatcaaaa tccttagatt taatggcttc tttttatgag gctgatgttt
     3481 ttatagatgt actaagtaaa tttgatgatg aagcaattgt tgtggcacat ggtagaccta
     3541 aagaaaagtc aatgtactgt gaaaatgtta taggtaaaaa ggctttaatt gaaaatgaac
     3601 caggagttat aaagtgctta aaaacaggag aaagagtaag agatattaaa gctttaacac
     3661 aggaatataa actggtcaaa caaaatattc agccaataac actagatgac aaagttttag
     3721 cagtactaat agttgaaaaa gatataagta gtgaagttaa gaatgagttt aatgctagtg
     3781 aagacgaaga aaataaaaat ttacatcagt ttataaaact tatcggaaat aatgactttt
     3841 tagttgataa tttaaattca gccatattaa tatttgataa aaatggaaaa ttaaaaatta
     3901 aaaatcgtat tgcaactgag atatacaaaa atcttgggtt tggatatgat atacaagata
     3961 tgcattacaa tgatttagat atagaaacca acacctttga aaatatatta atggatgaaa
     4021 attatgatga gacaaatgaa gtttcaatag gtaatatgtt ttttaaaata aagaccatat
     4081 acatcaaaga tgatgaattt aaggtttgca aaatagtaca agatataagt gatttaaaga
     4141 gaaaagaaga agaattagtt ttaaaaacag tggctataag agaagctcat catagagtaa
     4201 aaaataatct tcatacagta atatctataa taaaaaaaca aagtagatta tctcaaaatg
     4261 atgaagtaaa gcagtgttta gataacataa taaacagagt atttgcaata ctttcaactc
     4321 attatttact atctaaagaa gttgataata atatatctat acgagaaggt atcaatttat
     4381 tagtgtcaaa tattcaaggt ggctatttat ttagtaagga tatagatata cgtataactg
     4441 gtgaagattt taaaattagt ggagaaaaag caacagcaat acttttggtt ataaatgaaa
     4501 tactacaaaa ttgttatgac catgcatttt cggatagaga acatggtact atacaaatat
     4561 taataaataa agataacgac tatagaaata ttgctattat agatgatgga attggatttg
     4621 atagtaataa tataaataaa aatagcttag ggacatatat aatagatagt tatataacac
     4681 agatgttaaa aggaaatata gagagaaaaa gtaatgagaa tggtacagaa gtattaatta
     4741 ctatacctac acaaattaat atttaaataa taactcatct acaaagtagt agttttaatt
     4801 gagcaaagag gcttaaggtt attaatagat taagttaatt gaatgtattt ataaaaatta
     4861 taagtatata ttcaatttaa caggtattta ttataacttt aagcctcttt gcttgtttaa
     4921 gacatactta aattttagga ggtgagaaaa tgagagaaga gttattaagt gttggtattg
     4981 acattggaac aagtactact cagttaattt tttctaaatt aatagtagaa aatactgaat
     5041 caaatttttc ggtacctaga ataagtatag tagacaaaga aattatttat aaaagcaaag
     5101 tttatttcac ccctcttatt tcaaataatg aaataaatgg tcaacaaata aaagatatag
     5161 ttgaaaatga atataaaaaa gcaaatatat ctaaagagga tatacataca ggagcagtaa
     5221 taataactgg tgaaacagct agaaaagaaa atgcaaatga tgtacttcat actttgagtg
     5281 gatttgctgg ggacttcgtt gttgcaacag caggacctga tttagaaagt ataatatcag
     5341 gaaaaggagc tggagctcat atttattcaa aagaacattc aactacaatt gtaaatatag
     5401 acataggtgg gggaactagt aacatagcct catttaaaag gggagaagta accgatactg
     5461 ggtgtttgga tataggtgga agacttgtga agatagataa aataagtaaa aaaataactt
     5521 atatatcacc taaaatagat aaaatcataa aagataataa tttaaatctt caagtaggca
     5581 caatggctac tgtagaaaat acaaagccaa tagtggatat aatggttgac ttattaatgg
     5641 aaagcatagg attaaagaaa cagacacctt tgtttgattt tataataacc aataaatcaa
     5701 taagtataga tgaaaatatt aaaaatgttt cattctctgg tggagtagct gattatattt
     5761 actatgatgg agaaataaaa gattattttg aatatggtga cttgggaatt ttacttgggc
     5821 aaacaataaa aaattgtgaa taccttaaaa aagtaaatgt agttaaatct atggaaacaa
     5881 taagagccac agtggttggg gcaggttctc atactactga agtaagtgga agtacaataa
     5941 cttacactag cgaaaagttt ccacttaaga acttgccagt tttaaaatta tcagttgatg
     6001 aagaaacagg tacttcaaaa caaatcagtg aagctataaa aaataaactt aattggttta
     6061 aactagaaaa tgatttgcag aaagttgcta tagccatgat aggaaagaaa aatccaacct
     6121 ttttagatat acaagagtat gctaaagggt ttgtagatgg aatgcaacaa atcatagaaa
     6181 atgatttacc ttttatagta attgttgaaa atgatatggc aaaagtgtta ggtcaagcaa
     6241 tatatgcatt attaaattat gaaaaagaaa ttgtttgtat agacagtgtc aaggttgaaa
     6301 atggagacta tatcgacatt ggaaaaccta tagttgaagg taaagtttta cctgtggtaa
     6361 taaaaacatt agtttttaat taatagaagg aggtaagtaa atgattttaa atacaaaatt
     6421 atttggacgt acttatcagt ttaaaggctt atatgatgta atggctaaag ctaatgaaga
     6481 aaaatcagga gataaattag caggtcttgc agcagaatca gctgaagaaa gaatagcagc
     6541 aaaagttgtt ttatcacaca taaaattgga ggatataaga aataatcctg cagtggcata
     6601 tgaagaagat gaagtaacaa gaataataca agatgctgta aatgaaaaag tttatgaaga
     6661 aataaaaaat tggacagtat ctgaacttag agaatggcta ttggattcaa atactacaag
     6721 tcttgatata agaagaatat caagaggatt aacttctgaa atgatagcag cagttgcaaa
     6781 attaatgtca aatatggact tagtttatgc agctaaaaag ataaaggtta ctgctcactg
     6841 taatacaact ataggagaac aaggaacatt atcagcaaga cttcaaccta atcatccaac
     6901 agacgaccca gatggaatac ttgcatcact tcttgaaggt ttaacttttg gtataggaga
     6961 tgcagtactt ggattaaatc cagttgatga ctctgttgag agcgttacta aagtattaaa
     7021 aagatttgaa gaagtaaaac aaaaatatca gataccaact caaacatgtg tattggcaca
     7081 cgttactaca caaatggaag caataagaaa tggtgctcct gcagatttaa tattccaatc
     7141 tatagcaggt tctgaaaaag gaaatgaagc atttggattt aatggcgaca ctatagaaga
     7201 agctagacag ttagctttaa aacacggtac agctacagga cctaacgtta tgtattttga
     7261 aactggacaa ggttcagaat tatcttcaga agcacatcat ggaatagacc aagttactat
     7321 ggaagctaga tgctatggat ttgctaaaaa gtatgaacca ttcttagtaa atacagttgt
     7381 tgggtttata ggacctgagt acttatatga tgctagagaa gttgtaagag caggacttga
     7441 agaccacttt atgggtaaac tacatggctt accaatgggt gttgatgttt gttacactaa
     7501 ccatatgaag gctgaccaaa atgatatgga aaacttagcc atactattaa caacagcagg
     7561 atgtacgtat tttatgggaa tacctcatgg agatgatgtt atgcttaact atcaaacaac
     7621 aggataccat gaaacagcat cacttagaga aatgtttgga ctaacagcaa taaaagaatt
     7681 tgatgcttgg atggaaaaga tgggattcta taaagatggt aagttaacag agttggcggg
     7741 agacgcatct gtattattag gataggaggt atttaatatg aatgagaagg atttaaaagc
     7801 attggtagaa caattagttg gacaaatggt tggagaatta gatactaatg ttgtatctga
     7861 gactgttaaa aagacaactg aagttgtagt agacaacaat gcttttatag atgatataac
     7921 agaagttgat ataagaaagc aattactagt aaaaaatcct aaggatgcag aagcttattt
     7981 agatatgaaa gcaaaaacac ctgcaagatt aggtatagga agagctggta ctagatataa
     8041 aacagaaaca gttttaagat ttagagcaga ccatgctgct gcacaagatg ctgtattctc
     8101 ttatgtagat gaagaattta taaaagaaaa taatatgttt gcagtggaaa ctttatgtaa
     8161 agataaagat gaatacttaa caagaccaga tttgggaaga aaattttctc aagaaactat
     8221 aaataatata aaatctaaat ttggtacaaa tcaaaaggtt ttaatattag ttggagatgg
     8281 gcttagctca gctgcaatag aggcaaattt aaaagactgt gtgccagcta taaaacaggg
     8341 tctaaaaatg tatggtatag attcaagtga aattttattt gttaaacatt gtagagttgg
     8401 tgctatggac cacttaggtg aagaattagg ttgtgaagtt atatgtatgt tggttggaga
     8461 aagaccagga ttagttactg ctgaatcaat gtcagcatat atagcttata agccatatat
     8521 aggaatggca gaagcaaaaa gaacagttat atcaaatatt cacaaaggtg gaacaactgc
     8581 agttgaagca ggtgctcata tagctgaact tataaaaact atgttagata aaaaagcttc
     8641 aggaatagac cttaaataaa gtcgagggag tgtagaatat gaaaaatgat gtaattcgtc
     8701 ctaatatact aggaattaaa ataatttcga atataagtcc agaaatggca caaaaactag
     8761 aattaaaatc acatcacaaa agtttaggtt taataactgc agactgtgat gatgttacgt
     8821 atacagctct tgacgaagca actaaagctt cagaggttga tgttgtatat gcaaaatcaa
     8881 tgtatgcagg tgctgcaaat gcttcaacaa aacttgctgg tgaagtatta ggaataatag
     8941 caggcccaag cccagcagaa gtaagaagtg gtctaaatgc agtagttgat tttctagagt
     9001 atggagcaac ttttataagt gcaaatgatg atgattcaat agcatactat gctcactgcg
     9061 tatcaagaac aggtacatac ctttcagaga tagcaggaat aagagaaggt gaagcattag
     9121 cttatttagt agcaccacca cttgaagcaa tgtatgcact agatgcagca atgaaagctg
     9181 cagatgttaa gatgtgtgaa ttatttgcac ctccaactga aacaaacttt ggtggagcat
     9241 tacttacagg ttctcaatcg gcttgtaaag cagcatgtga tgcatttgca gaagcagtaa
     9301 aatcagttgc agacaatcca acaggatttt aaggatagtt agatggatgc tataggctta
     9361 atagaagtaa taggatatgt tgctgcaata gaagcttctg atgcttgttt aaaatctgca
     9421 aatgttaata ttgtaagaat tgataaggtt ggggcaggta ttgttacttt aacaatatgt
     9481 ggagatgtcg gtgctgtaaa agctgcttta gaagcaggtg aaatagcagc ttccagagtt
     9541 ggtacattaa gaacatcaca tattataccg agaatccata atgaagtaac aaatgcttta
     9601 ttcaaaacta aagaacctaa agtctgtgaa gtttcagaag ataaagaaaa aaactttgag
     9661 attacattgg aaataactga taatgtatcg aaaaatttat cagaaaaaga aaatgtaatt
     9721 ttagatgtca atgaaaacag tggagatact gaaaatttat atagtgatat agataaaaca
     9781 aacaaaaaaa ttgaaacagt tgatgatagt ttaacagaag ctaatgagga aaaaatagaa
     9841 aatactaatg ataaagaagt attggaacaa gttaaaatag aaaattccga ttttgataga
     9901 gatgacctat caaagaacag tgtaaaaaaa cttaaagcta tggctaagaa attagattct
     9961 tcaataactt ataaagattt aaatgttctt aaaaaagaag aattaataaa cttagttaaa
    10021 aagcttagca gaagggatag atagttatga cacttttaga taaagattta gtatctatac
    10081 aagaagtcag aactcttgta agaagatcta aagtggctca agagaaactt tctaaaatga
    10141 gtcaagagca aatagataaa atagtaaaag ctgttgtaga tgcagcattt aataatgcta
    10201 ctaaattagc taaaatggca aatgaagaaa ctggatttgg aaaatgggaa gataaagtaa
    10261 ttaaaaatac ttttgcatct atgggagttt atgaaacttt aaagaacaca aaaactattg
    10321 gaataattgg tgaagataaa gaagccaata cgatagatat agctacacca gttggagttg
    10381 tggcaggtct tataccttca acaaacccaa catcaacagt aatatacaaa actataatat
    10441 ctctaaaagc aggaaatact atagttttct ctccacatcc aaatgcaaaa aactgcatac
    10501 ttgaaacagt taatataata agtgaggctg ctataaaggc tggagctcca gaggattcaa
    10561 tattatgtat aactaatcca agtatagttg gaaccaatga attgatgaaa cataaagata
    10621 caaacttaat actagcaact ggaggagaag caatggttag agctgcttac tcttcaggaa
    10681 caccagctat tggagttgga ccaggaaatg gaccagcatt tatagataaa tcagcaaatg
    10741 ttaaattagc aatcaaaaga ataatagatt ctaaaacttt tgataatggt gttatatgtg
    10801 catcagagca atctatagta atagaaaaat ctatggaaga aactgtttta aaagagctta
    10861 aaaatcaagg tgcatatgtt ttaaatgaag aggaaagtga aagattagca aaattcatat
    10921 taagagctaa tggtactatg aatccacaaa tagttggtaa aagtgttgag gtaatagcaa
    10981 atttagcaga cttaacaaat atacctaaaa atacaaaagt tctagttgca agagaaaata
    11041 aagttggtca caatgtagcc tattcaagag aaaaacttac accaatacta gcgttttatg
    11101 ttgaagaaaa tgtagaagct attatgaaca gatgtagaga aatattgtta aatgaaggtg
    11161 caggtcatac attctcaatg catgtaaatg atgaagaatt ggtaaaaaga tttgccctta
    11221 atatgccagt atcaagaata cttgtgaata ctccttcagc acttggagga ataggtgcat
    11281 caacaaacct tgtaccagca ctaacacttg gttgtggtgc aataggtgga agttcaactt
    11341 caaacaatat aggaccactt gatttaataa atgttaaaag agtagcttat ggaatcaaag
    11401 aaatagaaga acttggaaga gataaatctc aagttgaaaa atcaatgttt gactcaatgg
    11461 ataaagaaga attaataaat cttcttgttc aaaaactagt caaagaacta gcataactaa
    11521 aataaacaat gaaacttatg gctatcgcta taaatttata aaaatataaa ctataaaaaa
    11581 ctataaaatt aaatttttgg aggataagat tatggcaagt gcaaacgcat taggaatgat
    11641 agaaactaaa ggattagtag gagcaataga agcagcagat gcaatggtta aagctgcaaa
    11701 tgtacaatta gtaggaaaag agcaagtagg tggaggatta gttactgtaa tggtaagagg
    11761 agatgttgga gctgttaaag ctgctacaga tgcaggagct gcagctgccg agagagttgg
    11821 agaactaata tcagttcatg ttataccaag accacacttt gaagtagatg ctatattacc
    11881 aaaggtatca gctgagtaat ataagaataa ttttaaaatt taaattttga ttgaaaaaga
    11941 ggctatctca aaaatgagtc ttactaataa actcaatgta ttttgaggta gtctttttta
    12001 tacatctaat aggagatgtg ataaaatggg tgttttaaca gaaaacgaag taaaaagaaa
    12061 aattggaaaa aacaaaaata atataactga attttatata gaaaaaggtc aaataataac
    12121 tccttctgca aaatcttatt tagctgagag aagtattgag cttaaatatg ttgataaact
    12181 ttcagatgta aacaatgaac ctaaagaagt tgtaaaagaa gtagttaaag agaaaaaata
    12241 taaatatact actgtatttg gcgctaaatt ggatgaaaaa ccagaacata tgactcaatt
    12301 aaatggaaat cttttagttt ttaaagacca taaaagaata atattgagag gaaaaataga
    12361 ttctcttgaa tcagaaatac taaaggtaca gatactttgt cagaaactag atatgccaaa
    12421 gcttgttaag gaattgcaag aagtattaga ctttgtaaga aatataatga gatgtgaagt
    12481 tttagatgaa aagctagaag actttaaact tttaggatta actcctcaag aacttagaga
    12541 acaatctcac tatcctaaaa aatactttgg tataggacat gaattcccaa gttatgaaat
    12601 gggagaagta gtagtagcta taaatagtat aagaagtaat acacgagagg ttgaacttgc
    12661 agcctatgaa gcatttaagg gtgagtatgg aaatgttcac agagaagatt taataaaggc
    12721 acttaataga ttatcgtcag tattttggat aatgatatat aaggttagaa ctaataagta
    12781 taagtagttg gaggacataa tggagaatct attaaatgaa atagtggaag aagtaataac
    12841 tagagttaaa aaagaagcat ttatagaagt tgaagcatca ggaagacacg ttcacttaag
    12901 tagagaagat gtcgataaat tatttggaga aggatatact cttacaaaac taaaagacct
    12961 atctcaacca ggtcaatatg catgtaagga aagagttaca ataactggtc ctaaaggaag
    13021 tataaaaaat gtagttgtac ttggaccatg tagaaatgaa actcaggtag aaatatcttt
    13081 aacagatggt tcaactcttg gactaaaagc tcctataaaa caaagtggag acttagaagg
    13141 tactttaagt attaaaatat caacacaata tggtgaagtt gaacttgata aggggttaat
    13201 ggttgctaaa agacatatac acatgactcc taaagatgct gaaaaattca atgtatgtga
    13261 taaagagata gttgaagcca aggtgatggg tcaaagacct ttaacatttg atgatgtagt
    13321 aataagagtg agtgacagtt ttaaaactta tatgcatata gactatgatg aagccaatgc
    13381 atgtggttat tcaaaaggaa ctgttgctaa aataataaag aaagcataaa tttaacctac
    13441 tatataaatt acctctaggg aggatttaat atgaattatg ataaccttgt agatttaata
    13501 acggaagaaa tctataaaaa aataaatagt aatgaaataa aaatatctaa taaacctaaa
    13561 gctgtgattg tatttgaaca agataacaat aaatttaact tattaaaaga tgaatttgaa
    13621 atactagaat ttgataaaag tataagggaa tgtgaaatag tgatagtttc aagactttgt
    13681 atgagggggc tatcgaatat agcacttgga aattcaacaa gtgatgaaga aagatttata
    13741 ctaaaaatga ttatgaaagg caaaaaagta tatgtcctag atgaaggaat tgaatacaaa
    13801 aaatataagg atactgcacc aaaagcatta tataagaaat ttatgtcatt tgaagatgaa
    13861 atatgtaaat ttggtgtaga aattataaaa gacttaaatg ttataactaa aaataagtta
    13921 aatataaaga aagaactaga aagtactaga ggttctaaaa aagaagatac aaaagtttta
    13981 gataaagatg ttttaaattt aaccagtgta gaaagtgtaa aaatagataa tgaatttagt
    14041 ttggatttaa ggaataaaaa acttatatcg gaagctgact taagaaaacc aactataaat
    14101 ggagttaaaa acatattagt taataaaaaa agtataataa caccattagc tgtagatttt
    14161 atgagaatac accatctcaa actaaagaaa ctgtaaggag gtaattttat ggatatagga
    14221 aaagttgtag gtaatgtttg ggctacaaga aaagatgaaa aattatgtgg ccaaaaactt
    14281 ttagttgtaa aaattttaaa aactaaagac acatacaagg aaggcttcat ggttgcagcc
    14341 gataatgcag gagcaggaaa tggagactta gttcttatat ccaaaggttc ctcagctaga
    14401 gagtcgattg aaaaatctca tgttccagta gatgcaacta tagttggagt agtcgattcg
    14461 tttgaggtgt ttgatgaata aaagtatagg agctatagag tttaaaagca ttgctaaagg
    14521 tatagaagta tccaatgaaa tgataaagaa atcttcagtt gatgtattgt acttaaaaag
    14581 tatttgccca ggtaaatttc taataatagt aggtggagaa acttcttata taaatgaatg
    14641 tgtagattat ggaataaaac ttggagaagg atatattgta gataacttcg taataaatgc
    14701 aatttcacaa gaaatattag atggatttaa aaataagtat caaaagctag atagtatagt
    14761 ctctataggc gttgttgaaa gtagtaaagt atgcgctgga ataaaaatgc ttgataagac
    14821 tttaaaatct ggcgacttag tattagtaaa acttcaatta tcttttgcta taggtggcaa
    14881 attagtatat gtagtagcag gagatttaag tagtttagaa tatgcattaa aagaaagtga
    14941 aaatgtagta agagaaaaag aagtaatata taagactgta ataccatcag tagacagtca
    15001 aataataaaa agtctaataa aataatgggg ggaattatga gtataaacga aattataatc
    15061 tatgtaatgg tagtcttcat ggtacttgga gctatagata aatgtatagg taacaaattt
    15121 ggtcttggag aacaatttga agaaggtata atggcaatgg ggtcacttgc agttgcaatg
    15181 gttggtgtta tatgtttagc accagttctt gctgatgtat taagaccaat tgtagtacca
    15241 gtatttaata tacttggagc tgacccagct atgtttgctg gaagtttatt agctaatgat
    15301 atgggtggtg cgccacttgc acttgaactt gcgaaagacc ctaattctgg gttatttggt
    15361 ggattaatag taggggctat gatgggacct acaatagtat ttataatacc agttgcgctt
    15421 ggaataattg aaaaagaaga ccaaaaattc ttagcaacag gaatacttgc aggtataata
    15481 acaataccta ttggagcatt cgttggtgga ttagttgcag gattcgaggt aatgatggtt
    15541 cttagaaact tagtgccaat aataatattt gcagtagtta tagcacttgg attattaaaa
    15601 ttcgaaaatg ctatgataaa aggattcaca tactttggaa aaggtgtagt aatagttata
    15661 actttaggac ttgctgctgc catagttgaa gctttaacag gaatagttgt aatacctgga
    15721 atgaaaccaa tcagtgaagg tatagaaata gttggagata tagcaatagt tttagcagga
    15781 gcattcccat tagtgtttgt tataacaaag gtatttaata aaccattaat ggctttagga
    15841 aaagttcttg gaatgaatga tgtatcagca gcaggccttg ttgcaagttt agctaactgt
    15901 ataccaatgt ttggtatgat gaaagatatg gacaatagag gaaaaataat aaatgtagca
    15961 ttctctgtat cagcagcatt cgtatttgga gaccacttag gatttacggc aggatttaat
    16021 tctaatatga taacaccaat gatagttgct aaattagttg gtggtataac agcagtattg
    16081 ttagctatgg ttatcgcaaa taagacacta aggaaggaga atgtataaga tggatatatc
    16141 aaatatagat aaaaatttaa ttgaaacatt agtaagacaa ataatagaag aaaaaataag
    16201 tggaactaaa gacacagtag attttgttag aaataaagac attagtggaa taacttctat
    16261 aaagttacca actgtaaaag ttagtgaatc tgatagatta gatacaggga atccaagtga
    16321 tgtagtttac acaaaagatt tatttacatt agaggaaagc ccaagattag gttgtggtat
    16381 gatggaaatg aaagaaacaa cttttgattg gactctaaac tatgatgaaa tagattatgt
    16441 aatagatgga actttagata taataataga tggaagaaag gtttctgctt cttcaggtga
    16501 attaatattc ataccaaaag gaagtaagat acaattttct gtacctgatt atgctagatt
    16561 tatatatgta acatatccgg cagattgggc atctcaaaat taataaaaat caagttaagt
    16621 taataataat caaataaata atttaaaata caaactctta aataaacaca catatttaaa
    16681 aaagctatga attaaaattc atagcttttt ttataaagaa tgttatattt tagacgtatt
    16741 ttttctatat ttactgggag acatattata ttttttatta aataatctaa taaaatgctc
    16801 tacatttgga taaccaatac tattttactg ataatctttt ccttatcact aatcttacat
    16861 tttaaaatat tatttttact cgtaatatta tcataagaat ttgtttctta tttaaaacta
    16921 attagaatta taaagtttat tgccatgtta ttaataaaat aaatccagtt aaatcgagtg
    16981 ctagttaaag taataagaaa cttttacaat ggtttccttt atcgattgaa ataaaagtta
    17041 atatggtata atatctaaag agagaatctt atcaattaga atatgcatag aaacaagtgg
    17101 cttaaaagga gacgttatat ggaagagata gtaaagaagg aacaagcaag tatgaatgat
    17161 atttttttgt atcttaaagc taaatcaaac tcattatata aatttgtaat attatataat
    17221 gagtttatca ataaacctaa agattatgga actggggaac ttataaacat ggttgaagta
    17281 catatgctaa cagcgattga agaaaatcca ggaataagtc caactcagtt agctaaaata
    17341 tggaacagaa ctaaaggtgc aatatctcaa actatatcta aattggagaa aaagggttat
    17401 ataactaaag aaaagaaaga aggaaatgca aaaactatat tactatatcc aaccacatca
    17461 ggagtagagt taagtaaggc acataaacta tatgacacca tagatgttac aagaacattt
    17521 gaatacttaa tcaaggagtg tacattagag gagatagata gtttttataa agtaattgat
    17581 ttgtttatta atttattaga agaatctccg caggaaaata atgattctga aaaagtgtag
    17641 ctaagattga agttgaataa agagaaatag atattataga agtagtactt aagtacacat
    17701 tatataaaaa tataataggt ataggattaa aaagtttagt ggtttaaagt ttaaccacta
    17761 aactttttta atgtctataa tctactatta tttaatttac taaaaataaa aaaactcatt
    17821 atattaagat gaaaatagaa aaataactag aaaaatacta taatatatca gcttaataca
    17881 gaaaaaagtt aaaaatattg agaaaaataa aaatgaaaac gttttatatt gacatagaga
    17941 aaaagtagtg ataccattta aatatagttt agtagctaaa ctatatttaa attataataa
    18001 ctattttagg gggatgttat gaatttaaaa caattaagtg aaaaatacga agaatatgta
    18061 attcaaatga gaagagaatt tcataagtat ccagaattat cttttaaaga aataagaaca
    18121 acccaaagaa taagggaaga attacaaaaa ataggggtcc cttttgtatc tattgaacca
    18181 aatatcgtaa tagctactat acaaggcaaa aatgaaggta agacaattgc aattagagga
    18241 gatatagatg cgcttccaat gcaagaagat tcagatgttg aatacaaatc agaatatgat
    18301 ggaataatgc attcttgtgg acatgatgct catggagcta tgttactagg acttgcacat
    18361 atgctaaatg atataagaaa agatttaaaa ggtagagtat tattagtttt tcaagcagct
    18421 gaagaagttg gtggcggaca caaggagata atacagtact ttaaggaaaa tggaggacca
    18481 gatagattaa tagctactca tgtttggtcc gatattgaag ctggtaaaat ttctgttgaa
    18541 ccaggacctc gtatggctgg tgggtctggt tggagaatag acataacagg tagaggaggt
    18601 catggttcta gaccagatca aagcatagat cctataaagc cagcttgtga tatagtttta
    18661 aaagtatctt ctataccagt taatcatata agtgtactag aacaatgtgt tgtacatgca
    18721 tgtgcaatac atggaggaac tacaatagga aatgttattc caaacaatgc agaggtaata
    18781 ggtggatata gatattttac tgaagaatca ggcaaaaaag tgtttgacat tataaagcaa
    18841 atgagtaatg gtgttggaca agcttatgga gtggatgtga aggttactca tactggaggc
    18901 ataggacctg ttatcaatga tgaagaagca gttaagatgg ctaaagaaat agtttctaat
    18961 atagatggat tggagttaga ttcttatgag ccaatatgtg catcagattg ttatggtgaa
    19021 atattaaaag agtattctgg tttttattgt tatctagggg taggtaacaa agaaaaagga
    19081 ataatatatg cacaacacca tcctaagtac aatatagatg aagatacatt aaagttaggt
    19141 tgcgaattta tggctaaata tgcagttgaa tttttaaata aaaataataa ctaaaataaa
    19201 tcgaatttta taaatggggg ggatgaaaat gcaaggtgca ttaaaggaaa ataagaaaaa
    19261 aagtggtgga ggatttttag gatggattga aagagttggg aataaaatgc cacatcctct
    19321 agcattattt acatatatta cattgatagt agtagcatta tctgcaatag caagcttttt
    19381 aggtttttca gcaacaagtc ctgctacagg aaagttaata aaagttatga acttaataag
    19441 tgcaaaaggt ttagtactat ttatgcaaga gtttgttaag aacttccaaa acttccctgt
    19501 gcttggggta gttttagtta tggcagttgc cacaggagtt tgtgaaaaga caggattttt
    19561 ttcaactgcc ataaaaatga gtttatcaaa agttaagggg aatgcggttg tatttataat
    19621 agcttttata ggagtatttg ctaatcaagc tggtgatgct gcttttgtat tagtaccaac
    19681 tcttgcagga gcaatatttt atggattaaa tagaaatcca ttagctggta tattctgtgg
    19741 atttgcatca gttggtggtg gatttgcaac agctgtaatt cctgggggat gggacgttac
    19801 gctaacgcca ataactgtat cagctgctca aactattcaa cctgcatttg atatgacatt
    19861 attagctagt tattatgcac tatttgtatc tgcgtttata gtagctacag tatcaacaat
    19921 agtaacggtt aaatttatag aacctaaatt aggtaagtat actggtaaac cagaaggtat
    19981 agatgataat caaggttttg aggtcacaaa agaacaagca aaagcagcaa agttagcagg
    20041 ttttacagtt ttagcttatg tagcgttatt aattgctttg tgtataccag ctaatagttt
    20101 tttaagaagt ccagaaggtt ctttaatatt tggagcacca cttatgtctt gcttacagtt
    20161 ttttatagta atactattct tcttgccagg gattgtgtat ggaatgaggg ttggtaaaat
    20221 caagactgta aaagacttaa atgatatatt atgtcaatca atggcagcaa tggcaccgtt
    20281 tatcgtactt gcaatagtta taggtcaatt cttaacgcta ttctcagtgt ctaatttagc
    20341 acaggtacta gctataaaag gtggagcatt actcaactca ttgccactac caccacaagt
    20401 tataatactg ctattcttag tactagtagc gtttataaat atatttgtta taagcggaag
    20461 tacaaaatgg atgatatttg gaccagtatt tgttccgatg ttaatgcaat taaacataca
    20521 tccagcattt actcagtttg tatatagatt aggagattct gtaactaatc atttatcacc
    20581 attaaatgca ttctttataa tacttttagc aacagtccaa aaatacgata aaaatgcagg
    20641 tatgggaact atattctcag ctatgatacc ttacactata ggttactttg tagtacttgc
    20701 agttttagta gtagtttgga tgacaatagg tataccagta ggaataggtg gacaagtttg
    20761 gttataaaac atatttaata attttgggag gaaagaaaat ggaaacaact gaaataagat
    20821 acaattttga tgaagtaata gatagaagtg gaacaaactg tcttaaatat gatcttaaga
    20881 gtcaatttat gccagaatct ccagaagatg cactaccact atgggtagca gacatggatt
    20941 ttgcatgtgc tcctgagata attaatgcaa tgcatgaaag aatagacaga aaaatatttg
    21001 gatatagctc tcaacaaact gatgattatt atgaagctgt atgtggatgg tttaagagaa
    21061 gatttgattg gaaaatagaa aaagagaata tattctttag cccaggggtt gtgcctgcgt
    21121 taggattttt catacaaata ttaacacaaa agggtgatgg tatcataata gaaaagcctg
    21181 tatactatcc ttttgaagat aaaataacta ataatgaaag aaaagtagtt aatagtcctt
    21241 taaagtttga agatggtcat tattctatgg attatgatgg atttgaagaa ttagctaaag
    21301 atgaaaataa taaggtgttt atattatgta gtcctcataa tccagttggt agagtatgga
    21361 aaaaagaaga acttagaaaa atagtagaca tatgtaagaa atatgatttg tggataatat
    21421 ctgatgaaat acattgtgat attattcgta aaagtgaaaa gcatatacca ttagaagtag
    21481 catgtcctga ttataaagat agaataataa cttgtacagc tccaagtaag agttttaact
    21541 tagctggtat gcaattatca aatatagtaa tcaataataa ggaattacaa gaaaaatgga
    21601 tgcttgaaac agattctaaa ttagctatat tcctacctaa tccattcgca atagttgcta
    21661 ctaaagttgc ttataatgat tgtgaagatt ggatgaatca agtaaatgag tatatagatg
    21721 gtaatattaa atatgtagaa gattttgtta atgagtatat gcctaaagta aagataataa
    21781 aatctcaagg aacttactta atttggttag actttagagg gtatggatta gatgcaaaac
    21841 agcttgaata tgtaatgctt aacaaggcga aggtattgct agatgaagga tacatattcg
    21901 gagaagaagg agcaggattt gaaagaataa atgttgcttc gcctagaaca attatagaag
    21961 agtgtatgaa tagaataaaa tgtgcatttg aaggattata atagtaatat ctaaataata
    22021 tatttgtgta ttttaaaaaa agactatgtt aaaaaaatat ttcaaagcat agtcttttaa
    22081 agttttatga tgagtttaaa aaatttcaaa acgttactat tattgataat cttcacttat
    22141 atgtttatat tagaaataat tccacaatat gctcattttc taaagttgca cttttataca
    22201 c
//
LOCUS       DAELXX010000068        21769 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.068, whole genome shotgun sequence.
ACCESSION   DAELXX010000068 DAELXX010000000
VERSION     DAELXX010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21769
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.068"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            506..1414
                     /gene="trxB"
                     /locus_tag="KPH29_002356"
     CDS             506..1414
                     /gene="trxB"
                     /locus_tag="KPH29_002356"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBG1467943.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCTTSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     gene            complement(1504..3327)
                     /gene="typA"
                     /locus_tag="KPH29_002357"
     CDS             complement(1504..3327)
                     /gene="typA"
                     /locus_tag="KPH29_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBG1467944.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            3539..3781
                     /locus_tag="KPH29_002358"
     CDS             3539..3781
                     /locus_tag="KPH29_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467945.1"
                     /translation="MLEVTKIIIKYFASESDTFIKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            3929..4120
                     /locus_tag="KPH29_002359"
     CDS             3929..4120
                     /locus_tag="KPH29_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HBG1467946.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
     gene            4411..6912
                     /locus_tag="KPH29_002360"
     CDS             4411..6912
                     /locus_tag="KPH29_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBG1467947.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQSVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVRNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNVLAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            7072..7740
                     /locus_tag="KPH29_002361"
     CDS             7072..7740
                     /locus_tag="KPH29_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1467948.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            7724..9001
                     /locus_tag="KPH29_002362"
     CDS             7724..9001
                     /locus_tag="KPH29_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBG1467949.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEKSELI
                     IRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVVFTVEFKN"
     gene            9402..9623
                     /locus_tag="KPH29_002363"
     CDS             9402..9623
                     /locus_tag="KPH29_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467950.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
     gene            complement(9912..10676)
                     /locus_tag="KPH29_002364"
     CDS             complement(9912..10676)
                     /locus_tag="KPH29_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBG1467951.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKAPTPDIVDILSEFNVNVL"
     gene            10950..11624
                     /gene="fsa"
                     /locus_tag="KPH29_002365"
     CDS             10950..11624
                     /gene="fsa"
                     /locus_tag="KPH29_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBG1467952.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILDEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GVKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVDKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(11746..13140)
                     /locus_tag="KPH29_002366"
     CDS             complement(11746..13140)
                     /locus_tag="KPH29_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HBG1467953.1"
                     /translation="MGIILKPKQFYMTVIVTIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTFVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFIFVFKKELHSIKFISNENLEGNKLHFSYSNLKKEFNLLNKEQKHVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     KENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWKFIGLEI"
     gene            complement(13218..14564)
                     /locus_tag="KPH29_002367"
     CDS             complement(13218..14564)
                     /locus_tag="KPH29_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG1467954.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGIQQMLFSIGNMTLQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGYWKTKAIVNHRINE"
     regulatory      14814..14916
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            15010..16386
                     /locus_tag="KPH29_002368"
     CDS             15010..16386
                     /locus_tag="KPH29_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBG1467955.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCLGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFM
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     gene            complement(16500..17645)
                     /locus_tag="KPH29_002369"
     CDS             complement(16500..17645)
                     /locus_tag="KPH29_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG1467956.1"
                     /translation="MNTFEQLKISSTLIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIVLDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     SRGNRSGNTISIITKKELDIIKDLQKEFSIVITPKTLQNGELIDIIK"
     gene            17897..18604
                     /locus_tag="KPH29_002370"
     CDS             17897..18604
                     /locus_tag="KPH29_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1467957.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            18651..20246
                     /locus_tag="KPH29_002371"
     CDS             18651..20246
                     /locus_tag="KPH29_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1467958.1"
                     /translation="MSNLSILIKTNIINEFKLNALKKADSREKQKIMGMILSVVIITG
                     LLIFYVTSISLSLVDILKQINQMEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMIIPAVVYFFNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFMFIGQISSRIKYKNLILIIGSIILMLLYTLLMTRLNSMSVEMLKN
                     SVSISETIRKIYPPIYYFVDALKTGSVVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKTYKFKSQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLIIGTILILL
                     FGADKVGELLKLNIDFNFIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKILMNFLLNAPLSILCLILVSIRLGFEIQFIIVSICLIASFSMFVALMGLLS
                     NLLYPNLDWKNEMAVVKRSASMIITMLVSLAYVIILGGIYVLLGISFLNIYIILASIL
                     TFILDFMIWRIISTKGVRIFKNL"
     gene            20349..21431
                     /locus_tag="KPH29_002372"
     CDS             20349..21431
                     /locus_tag="KPH29_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG1467959.1"
                     /translation="MRKLSKREKDFIQYGFLLFLICITTYLVLTTLDIKLIPKIIRLV
                     NKTYIFLGILTIIVYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASG
                     SQPMQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKV
                     LITFGMIMNVSMLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYID
                     DYNYSIKIFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQVFL
                     YMAISPIPTPGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLFTFG
                     THYTLNSWKKEDKVNSNLDKVIEKLN"
     gene            21580..21741
                     /locus_tag="KPH29_002373"
     CDS             21580..21741
                     /locus_tag="KPH29_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HBG1467960.1"
                     /translation="MKQDKQNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
BASE COUNT         8320 a         2566 c         3372 g         7511 t
ORIGIN      
        1 atggtatatc atataaatat aagtaaatag tgctaatatt taaactatta atcataattg
       61 gttcaacaaa agtatatagt taataatgtt taatttgaag aaattgttag tgtgaaatta
      121 catatatatt caaattaaat tcataaatat ttcacataat aatagtatac tgtaaacaaa
      181 aggaggtatt aaatatggga atagtactta taacagcagc attagttttg gttatgtatg
      241 gactttcaat tctatcatat atatcagaaa gcaatatatt agaagacact gtaaatgata
      301 accaataagg ttttgaatta tataaataag tgagaactgt aaataaaagt taatttttat
      361 ttgcagtttt tttatatata tgtaaaataa agttaattaa taaaaaataa tgttttgttt
      421 gtagtatata tggataataa tagtaaagta taagaatgat gagaataata tataagaaat
      481 aataatataa agagaggtaa acattatggt agatatcatt gtaattggtg ctggaccagc
      541 aggtttaact tcagcaattt atgctatgag agcagggtta agcgttacag tatttgaaaa
      601 aaatatttat ggaggtcaag ttgcaagtac ctctgaggta gaaaattatc cagcagttca
      661 aaagatttct ggggtagaat tttctaataa tatatacaat caagcagttg ctcaaggtgt
      721 tgatattcag tttgatgagg ttgaagagat aaatttagag ggaaaagtaa aggttgtaaa
      781 aacttcttca ggtgaacata aagcaaaggc tgttattctt gcaaatggag ttgaaagacg
      841 taaattaggt tgtgcagggg aacaagaatt tacaggtaga ggagtatctt actgtgcaac
      901 atgtgatgga gcctttttta aagataaaga agttgctata gtaggtggag gaaatactgc
      961 actagaagat gctttatttt tagctaataa ttgtacaaaa gtgtatttaa ttcacagaag
     1021 agatagtttt agaggtgaag aagtgttgga gaaatctgtg aaagcaagag agaatattga
     1081 gatactttac agtcatggtg tagaaaaaat agaaggagaa aagactgtat cgaaaataga
     1141 agtaaaaaac ttaaagactg aagaaaaaag aactatagat gttagtggta tttttattgc
     1201 tataggtctt aaacctaata ataaaatgtt tgaaaatgtt cttgatttgg atgaaggtgg
     1261 atatataatt tcagatgagt cttgtacaac atcagtagaa ggtgtatatg tagcaggaga
     1321 tagtagaact aagtttttac gtcaaataat aacagcagca tcagatggag ctattgcagc
     1381 cgttcaagca gctaattata taaatgttga ataatataaa tttcaaataa aaatagccat
     1441 ataaactgaa ataatttttc agtactatat ggctattttg ttaattagag gtaaatgtac
     1501 gttttattta ccttgtctag atttgttata tcttaatcta tcatgttcat ttaagaatct
     1561 ttttctaagt cttatagaat caggtgtaat ttcaactaat tcatcatctt ctataaactc
     1621 tagtgcttct tcaagagtaa atattctagg aggagataat tttatagcat catctgaacc
     1681 agaagcacgt acgttactca ttttcttgtt tttacaaggg ttgactacca tatcatcttt
     1741 tctggaattc ataccaatta tcataccttc gtaaacttct acaccagggt ctacaaacat
     1801 tacagctcta tcacttaagg cattaagaga ataccccata gtaacaccag gtccttgtgc
     1861 tattaaaaca ccatttcctc ttgaaggtat ttctccttta aattcttcaa atttttcaaa
     1921 tgaacgaact aaagtacctt ctccacgagt agcgtttata aattcacttc gatatcctaa
     1981 aagaccacga gtaggtgcta agaactctat ttttacatag tctccttcta ttgacataga
     2041 ctccatcata ccttttctca tgtttaattc atttataata gtaccagagt aaacttcagg
     2101 acagtttaca acaactcttt ctattggttc cattagtttt ccatcttctt tatgcattaa
     2161 aacttcaggt ttagaaacac caacttcata accttcacgt ctcatatttt caagtagtat
     2221 agataagtga agctctccac gtccagaaac tttgtatcca tcagtagtat caagaggttc
     2281 aactttaaga ccaacattta cttcaagttc cttttcaagt ctatctttca aatgtctagt
     2341 tgtaacgaat tttccacttt ttccaacgaa tggagagtcg tttactaaga agttcataga
     2401 aagagtaggc tcttctattt ttatcatttc cataggtagt ggactatcta aatcgcatat
     2461 agtttcacct atagatatat caggaatacc agctataaca actatttctc cactagtagc
     2521 ttcatcaact tcaacttgtt ttaaaccttc atacacagaa agttttgata cttttccttt
     2581 agatacaaca gaatcttctc tacatatagc aacttgtgta ttgtttttaa gagtaccttt
     2641 gtaaattcta cctataccaa gtctacctac ataatcatca tatgcaagag ctgatatttg
     2701 gaattgtaga ggttcattat cgtaattagg ataagcttca acgtgattta caatagtttt
     2761 aaatagagga gaaaggtctt cactatcatc atccatttca agtttagcaa taccttgttt
     2821 agcaatacca tatattatag gaaattcgca ttgctcatct gttgcattta agtctacaaa
     2881 taaatcgaaa acttcattaa ctacttcttc cgctctctgg tcttttttat caatcttgtt
     2941 tataaataat attggtttta aaccgaactc taaagatttt tgtaaaacga atctagtttg
     3001 aggcattggg ccttcactag catcaactaa tagaataact gtgtcaacag ttttcataac
     3061 acgttcaact tcagaagaaa agtcactgtg tcctggagta tcaactatgt ttattttgta
     3121 atcttcatag tttattgcac agttttttga atatatagtt atgcctcttt ctttttcaag
     3181 gtcgttactg tccataacgc agtctttaac tacttcgttc tttctaaaaa caccgctttg
     3241 agaaaggaaa gcatcaacca atgttgattt tccagcgtca acgtgggcta taactgcgat
     3301 atttataatt ttatgttttt gtgacatttt tgttgctccc ttcgattaaa ataacttatt
     3361 aattatagca caatatatta ttaggtacaa ggaggaatat attaaattgt attaatatag
     3421 tttaaattgg ataaaactgt tagttttgca tctgaaaatt attctgttta aaaaaatttt
     3481 ttagacaaat aataatctta atagataaaa agattaataa aagagtaaag gatgatatat
     3541 gttggaagta accaaaataa taattaaata ttttgcaagt gaaagtgata cttttataaa
     3601 agaagaagtt tgtaattttg aagaacttct taagtggtct cataaattta ttgaaggtaa
     3661 aaagatagaa gatgttttgt atataaagtc taaagaaaac tataaagtaa atgtgattaa
     3721 gtctttagaa attaaggaat tggaaatagt gagaaaacaa cagataccac cgtacatgta
     3781 gtattaataa tatataaaat taatatgtaa tactcacaat aggatataaa tggtatatat
     3841 ggtaataatt tatattaaaa aaacttttat aaagtttata tttgggtata aaagtattaa
     3901 ttagacatta attaggagga acttaaatat gataagagta tgtccaaact gttcaaatgt
     3961 ggatgtaaat aagctaaaaa ctttggttgg agaagaaaat gtaaagactg gttgtatagg
     4021 tcagtgtaga gcatttaaaa aagaagctgt tggaataata gacaatgagc ttgaaataaa
     4081 ggaaacagaa gcagagtttt ttgaagcatg taaagaataa attttaaata aaatataaat
     4141 tataataaat agagcatcta aattgtataa gcattaaaca atttagatgc tctattttta
     4201 tatatacatt taattatgag tatacttttc tattaaatac aaataaatat aactaataca
     4261 tttatttatt aaaaaaatat tgatttaata tacctatagg gggtataata aaaatataga
     4321 tttctaaata agtaatttat caatattaca tatcaaaata aaacgttaca aattagctga
     4381 aaatatattg aaagaaagga agattttatt atgagttcaa ataaagtggt agaaagttat
     4441 aaaattacag gtatgacatg tgcagcttgt gcaaaggcag tagaaagagt aactaagaag
     4501 atggatggag tatatgacca aagtgtaaat atagctactg aaaaacttaa aatagaatat
     4561 gataattcta aggttaattt tgatgatata aagcaggttg tagaaaaagc aggatatgga
     4621 attataaagg aagaaagtaa taaaaaaatt gatatgaaaa ttgatggaat gacatgtgca
     4681 gcttgtgcaa aagctgtaga gagagtggta aaaaaattgg atggagtaga aagtattagt
     4741 gtgaatattg caacagataa agctaatata gactatgacc catcaaaagt aaaattatct
     4801 caaataaaag cagctattga aaaagctgga tataaaccaa tagaagaggt aagaaataag
     4861 gttgatgtgg atgaagataa attgagaaaa gaaagagaaa tgaagtcttt atttgttaaa
     4921 tttatagttg caatagtatt tgcagtacct ctgttttata tagcaatggg acctatgata
     4981 ataaagccta tagggccatg gcctttgcca gaaattataa atcctatgac aaatactttt
     5041 aattatgcat taattcagtt aattttagta ataccagtaa tgatagctgg ttataaattt
     5101 tatataaatg ggtttaaatc acttttttct ttaagcccta atatggattc tttagttgca
     5161 ataggaacat tagccgcatt tttatatagt ctatatacga cactacaaat agccaatgga
     5221 caaattcaag gtatgcacca tcatcagttg tattatgaaa gtgcaggaat aataatagca
     5281 cttatcttac ttggtaaata tttagaatca aaatcaaaag gaaaaacatc agaagcaatt
     5341 aaaaagctta tggggctaca acctaaaact gcaatagtgt tagttgatgg taaagaagtt
     5401 gaaactccta tagaagaagt agagatagga gatatactat tagtaaaacc aggaactaaa
     5461 atacccgtgg atggagttgt aatagaagga tatacatcag ttgatgaatc aatgcttaca
     5521 ggtgaaagta taccagtaga aaaaaatgtt ggaagtaagg tgacaggagc tagtattaat
     5581 aaaaatggtg ttattaagtt taaggctgaa aaaattggtg gagatactgc tcttgcccaa
     5641 ataataaagt tagttgaaga tgcacaaggt acaaaagcac ctattgctaa acttgccgat
     5701 actgtatctg gatattttgt accaatagta attgcaatag ctgtagttgc atcattattg
     5761 tggtttttaa taggaggaaa agatatagta tttgtactta ctatatttat atcagtactt
     5821 gtaattgctt gtccttgtgc attaggatta gcaacaccaa ctgctataat ggttgggact
     5881 ggaaagggcg cagaaaatgg aatcttgata aaaggtggag aggcactaga atctgcacat
     5941 aaagtaaata cagtgatatt tgataagaca ggtactataa cagaaggaaa gccaaaagta
     6001 acagatatag tattaaataa taatgtaaaa gaagaatatc ttatcaagat tgcttcaagt
     6061 gcagaaaaag gttcagagca tcctcttgga gaagcaatag ttaagtatgg agaagagaag
     6121 aatataaaat ttgaaaaagt tgataatttt aaagccatac caggtgctgg tatacaagtt
     6181 actattaatg atgaaagtat attacttgga aatagaaagc ttatgaatga caataatata
     6241 aagcttgggg atttagaaga aaaatcaaat gtattggcaa gccaaggtaa aacacctatg
     6301 tatatagctg tagatggaaa tttatcaggt ataatagcag ttgcagatgt agtcaaagaa
     6361 agtagtaaaa aagcaataga aatacttcat gatatgggaa taaaagttgc aatggtaact
     6421 ggagataatg cgaaaactgc aaatgctata gcaaatcaag taggtataga tatggttttg
     6481 gcagaggttt taccagaaga taagtcaaaa gaagttgaaa aactacaaaa tcaaggaaag
     6541 tttgttgcaa tggtagggga tggaataaat gatgccccag ctcttgcaaa agcagatata
     6601 gggatagcaa taggaagtgg aacggatgta gccattgaat ctgcggatat agtacttatg
     6661 aagagtgatt taattgatgt tccaacagca attaaactta gtcatgaaac tataaaaaat
     6721 ataaaacaaa acctattttg ggcatttgga tataatacga ttggtatacc tgtagcagca
     6781 ggaatattat atgtatttgg aggaccttta ttaaacccaa tgattgcagc agcagcaatg
     6841 agcttaagtt cagtttcagt cgtatcaaat gcacttagac ttaaaaactt caaggcatat
     6901 aagagagatt agattaataa attaaataaa atgtatccaa aagagctatc tttaaattaa
     6961 aaggattaat ttacaaatta agatagctct tttttcatgt aaaattcaca acaataagat
     7021 aaaatataag taatatacga aattttaaat aaaatagaaa ggattgatag aatgaattcg
     7081 tcaatacttg taattgaaga tgattcaaat atacaagagt taatatcaga atttttaagt
     7141 gcagaaggtt atcaagttga tacagcaaat gatgggttgg aaggtataca aaagtttaag
     7201 caaggaagtt atgatttagt aatattagat ataatgatgc caaacttaga tggttatgga
     7261 gtttgtaaaa tgataagaaa gtcatcaagc gttccaatta tatttttaac tgctttaaat
     7321 gatgaaggag accaacttaa agggtttgac ttagaatgtg atgattatat aacaaaacca
     7381 ttttcattta atcttcttat aaaaagagta gaagcaattt taaggcgaag taacaagact
     7441 ataaatgaca aatttatagt ttttgaaaaa ttaaaattag atttaaacac atatattgca
     7501 gagatagatg gagaacctat agaacttaca ctaaaagagt ttaatatatt aaaagctttg
     7561 atagaaaagt atccacaagt tataactagg gaaggtcttt tggatagtat atggggttat
     7621 gactattatg gagatactag gattgttgat gcacatataa aaaatataag aaaaaaaata
     7681 tcgttaccat atattaaaac tgtaaaagga ataggatata cacttgagaa ggatatttga
     7741 taagtgggaa aagttaagta taaagtataa gttattttca ataacgacat cattattaat
     7801 agcgttagct ttaataatat atctaatttt atatttttta ttgccatcat attatcatga
     7861 gtataaaatt gagtctcttc aagaaagttt aaaatcttta gtagatagtt caattcattt
     7921 cgatacatat actttagaag aaagacttta ttatatggct aaagaccaaa atttggctat
     7981 tttattaaaa gataaccaag ggaaaattgt atatggtaag aatgaagtag ttattttaag
     8041 atatagtaag tacatgatta atagtcttga ggatgagtat agaacttcta tacctatata
     8101 tactaaagat gcaaaagatg gtccatatac tttagaactc gttatgccat tacaaccaat
     8161 tgacgaagca aacgaagtta taagaaaact aatgccatac atcatatcaa tagcaatatt
     8221 gatagcgatt attggagcat atatatattc aattgtaata acaaagcctc ttataaatat
     8281 aatagagagt gagagagaac aggaatatag aaggaaggat tttgttgcta caatatcaca
     8341 tgaactaaaa actccaataa ccataataag tggtcaaatt gaaggtatga tttatagtgt
     8401 aggtaagtat aaggataggg atacttatct gaaaaaatca tatgaatgta cacaagaatt
     8461 aaaagattta gtaaatgaaa tgatagaggt ttcaaaatca gaaatacttg aaaaagattt
     8521 gaaattggta tcaataaata taagtgaatt attaaataga cttgtaaaga gacaagtatt
     8581 tttaatagaa gaaaaacata tgaaaactat tttaaaaata gaagaaaatt tggaaattaa
     8641 agcagaccaa gagagaataa ctaaggcaat caataatata ataaataatg ccataaaata
     8701 ttctcctgaa aaatcagagc ttataattag actctatgat aaaaataaac gaataagtaa
     8761 aaagacttct aatcaaagag tggttttaga aattgaaaat actggtgtaa ctatcgagaa
     8821 acgttattta gaagagatat ttaatccatt ttatagaata gaaaaatccc gttcaagaaa
     8881 aactggagga agtggacttg gactttatat agtcagtcaa atttttaaaa gtcatgggtt
     8941 tgattatagc ataaaaaaca aagaaaactc tgtagtattc acagtagaat ttaaaaatta
     9001 attacttatt ataaagaata tataactttc aataatagtt cattttaaaa tcacaaaaaa
     9061 ttcataatga aactgtatta tataaatata aagaagctta aaaccataaa atacaccaac
     9121 tataatgatg agatttcctt acatcctcca tataagtcat cattatagta acccaacaat
     9181 taagttacta tgaatttaaa aaataacacc gagcatataa tctatgctta gtgttatttt
     9241 tattttgaaa taaaaaatat ttaatttatt gaaatgtata gatttagaca gtttaaacta
     9301 tatttaagac ttaaaaatta taattgatat tacaaataaa tatatgtact ttgggaaaaa
     9361 taaatatagg aagtattata tttacagagg aggtagtttt gatggataga ttaaaaagag
     9421 atatgaaaaa tatgaaagaa gatttaaaag aaggtgttaa aaagacagct tatgttatga
     9481 aaaatacagc tgaagatttg aaagatgatg cacaaggtgc tatggtaaac ttagaaatga
     9541 aaaaagatga gatgatggaa agatatgaac aaaagaaatt ttctaaagaa ttagagaagc
     9601 aaatgaagaa tgaaatgaaa taagctttta tttatgaata acaaattatt gtattcatat
     9661 aaaaagtagc cagtaatatt aaatagttta tttaaggaaa tagactttct aaaaataact
     9721 gtattagatt tagaaagtct atttcaaatt agttttataa atagacataa ggataaagta
     9781 gatgttatag aactagattt taaaaaatag atactaagga taaaataaat attatagaaa
     9841 tatgttttta agaatagtta tcaaataaaa atttatattt ataagaaatc ctataaaata
     9901 tataattaaa cttataaaac atttacatta aattctgata aaatatctac tatatcagga
     9961 gtaggcgctt tttctgtaat tatagaacta atatcttcta aatgagcaaa cttgaaattt
    10021 ccatcaaatt taaacttttt atcttccata actataaata cttttttact tgaatttatt
    10081 atagccttct tcgtgagacc atcttcaatt tcaaaagttg taagtgaaga gttgaataca
    10141 tctactccac aacttcccat aaaagtctta tcaaacttat atttcgatat aaactctata
    10201 gttggagtac cgacaaaacc gtctaaactg agatttaata aacctccagt ggaaattata
    10261 tttaaattat ttggttttga agtaactaca tttagtatat ccaacatatt tgtaacaagt
    10321 gttacttttt tattactttc agctaacaac tttgcaagca agatgttaat tgtagagata
    10381 tctaaaaata tagtttctct atcttcaatt aaattaaatg ctttttctgc tattattttc
    10441 tttgttggaa tattaatttc ttttctgtct tttgtatcct gattctctag tgtttctcta
    10501 gctagtacag ctcccccata aattcttctt attgaagaaa tcttttctat attttttaaa
    10561 tcttttctta tacaatcttc tgtaacatta aatttagaac tgagctcttt gacagtaact
    10621 ctaccttctt tttttaggat atttaatata gcttggattc tttcttctgc aaacattttc
    10681 tacctctttt cgcaatatag tattatagta aatattataa gataaaatac aatatatcaa
    10741 ctattttaac atgaaataca aaattaattt aatataaaaa gtatgttttt tctaactata
    10801 tatttagaat ttagtaaata aaaaaattat aaaactaaga aggaaaagca aaacaataat
    10861 aaaaaataaa aaacaataag caaaatgttg aaaataatac caaatcaata tataataaga
    10921 ataaagaaag attaaagggg gatattttaa tgcaactaat cttagataca ggtaatgtgg
    10981 aggagattaa ggagttatgt acttgtttac caatagatgg tgtaactaca aatccaagta
    11041 ttgtttcaaa agaaaaaaag aattttaagc aacttataaa tgaaattggt gagattatag
    11101 gtgaggatat gcctatacat gctcaagtgt taagtacaaa gtatgaagaa atattagacg
    11161 aagcattata tataagtagt ttgagaaaaa atatatatgt aaaaatacct gtaacacaag
    11221 atggtttaag agctataaaa gaccttcaca gaaaaggtgt aaaaataact gctactgcaa
    11281 tattcactgc tcatcaaggt tttttagctg caaaggctgg agcaaattat attgcacctt
    11341 atgtaaatag gcttgataat atatctggtg atggagtagc tatggtttca gaacttataa
    11401 aaataataga tacttataaa atggatacaa aagtacttgc agccagtttt aaaaatgcac
    11461 aacaagttat agaattaatg caacatgggg tgcatagtgt tacagtacca tatgatattt
    11521 gtaaatctat gatgaatcat ccattaactg attggagtgt agataagttt atagaggatt
    11581 gggagaatac ttttggaaaa ggaagtaaaa caaataatat ttagtattat ggtaaaagag
    11641 tagttttgtc caatttcaca agaacttata taaaaagatg aaaatatgaa ttttcttttt
    11701 aataatttcg ttattatctt aagttaattc atattttcat cttttttaga tttctagtcc
    11761 tataaatttc caccaaggga atgttataca tattattata aagtaggtta aaggaatcaa
    11821 agttaaaccg tatttgaaaa taaccttgct attatacaga ttttcaccat gaccaaccat
    11881 tattgtagca tgatgaaatg gtaatatgta ctgtatgctt actataatgt agacaaatag
    11941 acataaagta acactatttt cctttagtat atgtaaactt ccaagtaatg gtattactac
    12001 agatgatgta gtgacagcac ttccaagaca tatattcaag attattgtga gagttatcaa
    12061 aaacaatatt gaaataattt ctgaagagtt tggtataaac tttatcaaat aatttcccat
    12121 tatatctaca actccagagt actttaacac tcctccaatt gaaaatgctg ctataaaaaa
    12181 aataaggacc ttccaattta tttcttttaa agtactaaat ccaactattt ttcttagtaa
    12241 taaaagaagt acacaaattg acattataat taatgtgttt atgttatgta tgaactgagt
    12301 taaaaacata aaaaacatta ttcctaggca taaaaatacg tgtttttgct ctttgtttag
    12361 caaattaaat tcttttttta aattagaata tgaaaaatga agtttattac cctcaagatt
    12421 ttcattagaa ataaatttta ttgaatgcaa ttctttttta aagacaaaga taaataaaaa
    12481 ataagttata aaacaagtaa ttatagttgg aacagacata taaaaagccc attgacccca
    12541 atttatatta caatttccaa gttctagtac aacataattt agaagggtgt ctccatttac
    12601 aaaaaacatt gatgtaaagg tagatgctgt aaatatacta aataaaagta tagattttga
    12661 atcttcagtt atctgttgtt cttttaaaaa ctccttgtaa aaagcagata caagaataac
    12721 ccttggaaat ggttgtggga taaaaaatat taaaagaata cctaaaatat acgataaaag
    12781 aatcatcttt ttaggggtat ttacagtatt taaaattaac ttctcagaaa taatttttgc
    12841 gacacctgtt tttgtaactg cattagtaat tatgtatgca agaattatta tgtaaaaatt
    12901 ttctgtaaat aaaaactgta aaataacctt tattggtgca atgttaaata taaacgctag
    12961 aattacaaaa aacaatgcaa caaaagtttt ttcaactgag tctacagccc atatcaagat
    13021 agttaaaaaa gataagcata tagaaaattt tatgttgaaa ctgtaattaa gagagaagta
    13081 gagaattatg gtaactataa cagtcatata aaattgtttt ggcttaagaa taatacccat
    13141 ttttatattc acctccataa tttattatat cataatggag gtgaatacaa tgaatgtgaa
    13201 tacattttat aaaaagatta ttcattgatt ctatgattta caatagcttt agttttccaa
    13261 taccctttct tatagtagat aactgtaaca ataagaccta aagtccaacc aaaaggaata
    13321 cccatccaaa ttccgtctgt tccaaaaaat ttagaaagca tataagaagc tggaagtctt
    13381 ccaagccata aagatactat tgagcttata agtgcaaaca tggaatcacc agcacctttc
    13441 attgcacttg aaagtacaaa agaagttcca ataaataaaa agaatggtcc tattataaat
    13501 aagtaagaag agccaatttt aatgacatct ttatcagtat tgaatagagc aattagattt
    13561 tccctaaata gcaaaattaa agcaataact gtaactgcta aaactaaggt catttttata
    13621 gattctcgaa tgcctttttt aaccctttca ttttctccag cacctatgtt ttgagcaaca
    13681 aaagtagaaa cagcagaacc aagattcata ataggtaagc ttataaaatt ttctatccta
    13741 agaccagcac caaaagctgc catagcagat gtaccataac tattaactaa aaattgaaga
    13801 gtcatatttc caattgaaaa caacatttgt tgaattccac ttggaagtcc taatctaagt
    13861 ccatctttaa atattttgtt atcgtataca attcctttga ttttaaattt aagcacttca
    13921 tgttttttat ttaaataaat tatacttatt ataaatgata ctgcctgagc cattatagtg
    13981 gcaagtgcaa cgccctcaac acccattctt agcactacaa taaaagttaa gtctaaaact
    14041 atatttagaa ttgtggctat tatcagaaaa taaagtggag ttactgaatc tccaagtcct
    14101 ctaagtattg cacttactga attatatcca gcagaaaata atattccact aaatattata
    14161 gtaagataca attttgattg agcaaaaacg gattctggtg tattcataag cttaagcatt
    14221 gggccaccaa atactattcc aagaatactt attaatatag aaccaataaa taaaagtata
    14281 tatgtagtat caatagtagc ttttaccttt tttaaatctc ctgagccata aaattgtgac
    14341 actaaaatag aaaatccaag gctaacaccc ataagcgctg ccaccaataa aaacattata
    14401 gggttagctc cagagactgc cgccattgct tcttttccga taaatctacc aactattata
    14461 ctgtcagctg tattgtatag ttgttgaaat aaactaccaa taagcatagg catggcaaaa
    14521 aagaatatgg attttccctc atgacctgta gttaaatctt tcaccttatt cctccaaata
    14581 aaaaaattat taatttatta tagtataatc ttaacataaa ctttatatcc aaactataaa
    14641 taaattgtat caaaatatca tatatttttt taaaatgtaa agaatataat tataatttga
    14701 atttaataaa tcaatattta atataatcga atattaatac aaaatcaata caatatatac
    14761 gtatatattg acaaaattaa ttatataaat taaaataata ttcgtaaaga aaaattcatg
    14821 ttttgtactg tataacatat aattttgaca atatgggtca taagtttcta ccggaatacc
    14881 gtaaatattc tgactatgta tactattatt tagttaatta aatatttaat taactgaatt
    14941 atgaaggata cggctcagct tatcaactac tgtgtcgttt ttttgtaaaa tttaatagga
    15001 ggtgttgtta tgcaaacaac aaacagtagt actatgcaga aactttttcc aattttgact
    15061 aataaaaatg tcgacatgaa gaaggaaata attgcaggag taactacttt tttaactatg
    15121 gcttatataa tagctgtaaa tccaaatatt ttatctgaga caggcatgcc tgcaggtgca
    15181 cttgttacag gtacttgttt agcagcagca tttggatgta ttttaatggg tgtagttgct
    15241 aaccttcctt ttgctttagc atcaggtatg ggtcttaatg cattttttgc atatacagta
    15301 gttttacaaa tgggtgttcc ttgggaagta gctcttacag cagtatttgt agaaggtatt
    15361 atttttatag tattatcagt aagtggagtt cgtgaggctg ttgtaaatgc gataccaaaa
    15421 aatatgaaat tagctgttac aggtggtata ggaatattta ttgcacttat aggtctagtt
    15481 aacagtggaa ttgttattgg agaccaagcc actcttatca aaatgggtag atttacacca
    15541 gcagtaataa taacttgttt aggattaata attattgctg tattagataa aaaaagagtt
    15601 aaaggttcta tactttttgg aatagtagtg agttcattac ttgcttgggc atttgctttt
    15661 atgaatccag agtatgcaca aaaattaggt atatatttac ctggtggatt atttaaattc
    15721 gaaagtttag caccaatagc aggtaaaata gatttaggat atgtactaca tccaacaaat
    15781 ataggtggtt ttttagtagt agtttgtaca tttttatttg ttgatttttt tgatacagta
    15841 ggtacattag ttggtgtttg ttctaaagca aacatgttag atgaaaaagg gaatgttcct
    15901 aatgtaggta gagccttact tacagatgcg gttgcaacta cagttggtgc aggacttgga
    15961 gtatctacag ttacaactta tgttgagagt tcaacaggag ttatcgcagg tggaagaact
    16021 ggatggacag caataacagt tggaatttta ttcttagctg caatgttttt ctctcctgta
    16081 tttatagcaa ttccatcatg tgctacagca ccagctttaa tatatgttgg atatttaatg
    16141 cttggtacag ttaaggatat agagtttgat aatataactg aaggtgttcc agcttttgtt
    16201 actattgctt gtatggcatt aacatatagt ataggtgatg gtttaacttt aggtatatta
    16261 acttatgttt tcgtaaatat attttataat atatttggag ctaaaaaggc agaagacaaa
    16321 aaacatgttt ctgtagtaat gattgttctg gcaatattat ttataataaa actttggcta
    16381 ttataatagg tattatttaa aattttaatt taatataaaa taaagggcta taatttatta
    16441 tcaattgtga tgataaacta cagctctttt gtatttgttg tattttcgta tttattgtat
    16501 tatttaataa tgtctattag ttctccattt tgtaaagttt taggtgtaat tactatactg
    16561 aactcttttt gcaaatcttt tataatatct aattcttttt ttgttattat tgagattgtg
    16621 ttaccacttc gatttcctct agaagttcga ccacatctat gcaggtattc atttttactt
    16681 tttggaaaat ccaagttaaa aacatgactt acatccacaa tatctagtcc acgagcagat
    16741 aaatcagttg taattaaaat ctttgcttta ccaagtttaa atttatttat tgcattcttt
    16801 ctatcttctt tttccatatt tccaaaaatt cctattgctt tgtaattatg ataatttagt
    16861 tttgatacta ggacttctat atttttttca ttatttacaa atacaatagc tttttttgga
    16921 tttgtagctg ctaatgcctt tctaagaaaa gtaaatttat cccttatctc acctaataaa
    16981 tatgagtgat tgatgttagg atttatttgt gatttttcct ttgtttttat tatttcaaac
    17041 tccttcataa gtttatcaca aatttttata gtgctatcag taagacttgc agagcatcct
    17101 ataatttgtc tatctcttaa cgttgttctt attatgtctt ctatacaagt tatatttttt
    17161 ccattcaata gattgtccac ttcatctaag actatagttt ttatattatg tgattttagc
    17221 ttcttttgtt ttatcaagtc taaaactctt ccacaagagc caattactat gtgtggtttt
    17281 actgctttta tattttttat ttgtttttgt atattgacct ccccaattaa agctaaagaa
    17341 gtaacggaga gttctgcatt tttagccaat aattcaacct ggtttgtaat ctgcattact
    17401 aattcatgcg taggtgcaag tataagagct tgtgtttctc tttttgatgt atcaattttt
    17461 tcaaatatag gtaaaaggta cgctagtgtt tttcctgttc cagtctgtga attaatcaat
    17521 aaatccttat tttgaattat attccctata acaagtgact gaacttctgt tggagaagtt
    17581 atgtcttgtt ttttaagtcc atctattaga gtggaactta tttttaattg ttcaaatgta
    17641 ttcatatttt gctccttata tttaataatt atagtcaact attttttatt atacattgtt
    17701 tattcaaatc gaactatata ttcttttata attatgtgta atttgaaagt aaatatcaat
    17761 tatatttata cctttatatt tctgatacct ttgcatttta tagatttcta aaataaacaa
    17821 ataaaagttg taattgtttg atatttgtta tatattatat ataacaaata tcaaaactat
    17881 aatatggagg gaaattatgt taaaagtaat caatgtatct aaatcatata aaaataaaaa
    17941 agtagtaagt agtgtctcat ttgatgtaaa tgatggagag ctttttgggt ttataggaca
    18001 taatggagca ggtaaaacta ctactattaa agcaattgtt ggaatacacg attttgaaga
    18061 gggagaagta ctaataaatt ctaaatctat aaaaaaacag cctgttgaat gtaaaaagga
    18121 aatggcatat atacctgata atccagattt atacgagtca ttaacgggta tacaatacct
    18181 taattttata gctgacatat ttgaagttgc caaagacaaa agagaagagt taataaaata
    18241 ttattcaaat aagtttgaac tcaataaagc attaggagat ttaatatctt catattcgca
    18301 tggtatgaaa caaaaattag ctataatttc ggctttgata cacagcccaa aaatcttaat
    18361 tcttgatgaa ccttttgtag gtcttgaccc aaaagcttcc tttatattga aggaaattat
    18421 gagggagttt tgtgacaaag gtggttgtat atttttctca acacacgttt tagaagttgc
    18481 tgaaaaaatt tgtgataaga ttgccataat aaaagatgga aatataattg cttatggaac
    18541 tacagaagaa gttaagggaa gtaattcact tgaaagtatc tttatggaat taatagagaa
    18601 ataaaatgtt ttttatacaa tatagtgtct aaagtgagga atgataaaat atgagcaatc
    18661 taagtatatt aataaaaact aatataataa atgaatttaa attaaatgcg ttaaaaaaag
    18721 ctgatagtag agaaaaacaa aaaataatgg ggatgatact ttcagttgtt attataacag
    18781 ggttattgat tttttatgta actagtataa gtctttcttt agttgatatt ttaaagcaaa
    18841 taaatcagat ggaaatgcta cttatatttg gatttggatt atcaacatta acaactattg
    18901 ttacatcatt atataaaacg tcatcatatc tgtttcaagc aaaagattta gaccttttga
    18961 ctagtttacc tataaaagaa tctactgtac tagcaagtaa gatattaatg ttggttggag
    19021 caaactattt attttcttca atatgtatga ttataccagc agtggtttat ttttttaatt
    19081 ctgatgtaag tataacgtat ttcccttatt tgatactgct atttttagct ttaccattat
    19141 taccaatagt tgcttcatca ataattttta tgtttatagg acagatatca tcaagaataa
    19201 aatacaaaaa tttaatatta attataggta gtattatatt gatgttgctt tatactctac
    19261 ttatgacaag acttaactct atgtcggttg agatgttaaa gaatagtgta tctatttcag
    19321 agacgattag aaaaatatat ccacctatat attattttgt tgacgctctt aaaacaggaa
    19381 gtgtagtttc ctttttagca tttatagcaa tttcaataat accatttttt atgtttgtaa
    19441 cattatttgc aaaaaaattt aaagatataa atgctaagat gagtgaagga tataagtcaa
    19501 aaacttataa atttaagagc caaaaaactt caagaccttc aaaagctcta ttaaaaaaag
    19561 aatttaagag atatacttct tcatttatat actttttaaa ttcatctgca ggtttgttat
    19621 tcctaataat aggtacaatt ttaatattat tatttggagc agataaagtg ggtgagttac
    19681 tgaaattgaa tatagatttt aattttataa aaatacaatt attgggagta ttcttcttta
    19741 taatagtaat gaattgtaca acatactgtt caatttctct tgaaggaaaa aatttatgga
    19801 tactgaaatc ttctccaata gatgaaagag aaatattctg gtcaaaaata ttgatgaatt
    19861 ttttattgaa tgcaccatta tctattttat gtttaatatt agtatctata agattaggtt
    19921 ttgaaattca gtttattatt gtatcaatct gtttgattgc aagtttttca atgtttgtgg
    19981 cattgatggg attattatca aatttattat atccaaacct tgattggaaa aatgagatgg
    20041 ctgtagtaaa aagaagtgca agtatgataa ttacaatgtt agtatcatta gcttacgtaa
    20101 ttatacttgg aggtatatat gtattacttg gaatttcgtt tttaaatata tatattattt
    20161 tggcaagtat acttactttt atattagatt ttatgatatg gagaattatt tctactaaag
    20221 gagtaagaat atttaaaaat ttataggtgt atttatgtca aataaagact tatctttttt
    20281 agtcttgcaa ataataggtt aagtagtata ctatgtatag tgatatgaaa aaatttagga
    20341 gaaaataaat gagaaaatta tccaaaagag aaaaagattt tattcaatat ggatttttat
    20401 tatttttgat atgtattact acttatttag tattaactac tttagatata aaactaatac
    20461 ctaaaattat aaggctagta aataagactt atatatttct tggcatattg acaattatag
    20521 tatatatgat aattgaatct attgtaactc atttgataat aaatgcagtt caaaaaacaa
    20581 aagtaaaatt tattggtatt aaaatggcta caatgggatt ttactataac ttagttactc
    20641 catttgcatc tgggagtcaa ccaatgcaaa tatatgcact tacaaaatat gatgtaagcc
    20701 ttagtaaatc agtggctata gttactaata aaactgtagt atttcaaagt acagttactg
    20761 tattttgtgg aattttaatt ttgctaaata agtcattact gatggagcaa gtacattctg
    20821 tgaaagtctt gataactttt ggaatgatta tgaatgtaag tatgttgtta ggtggactat
    20881 taatagtatt tagtccaaaa caagtaaaaa tgatagcaga agttttgatt aattttttat
    20941 ctaaatttaa aacatttaaa tttttagatt caaaacgtga tagaataaat cattatattg
    21001 atgattataa ttattctata aaaatattta ttaaaaatgt aagaatctta gttttaagta
    21061 tattacttac tgtgttacaa ttaatcgcat atttcagtat agtttattgt gtgtataaag
    21121 cctcaaattt aagaggagtt tcatatgtac atataatgac attacaggtc tttttatata
    21181 tggctatatc accaattcca acaccaggaa atgttggagc taatgaggtt acatttttta
    21241 ctatgttctc caatataatt ccaaaagaat tattggggta ctctgtactc ttatatagtg
    21301 ggtttgtata ctattttata ttaatagttt caggtttatt tacatttgga acgcattata
    21361 cattaaattc ttggaaaaag gaagataaag taaattctaa tttagataag gttattgaaa
    21421 aattaaatta ataaaataat tagggaaatt ctatatataa atatggtgtt gagtaagcta
    21481 tattatattg agatatttcc cttttttgat taaaattata gaaatttgaa ataataagta
    21541 ataaacagaa aaagtgatat tgttttgaaa ggagaaacta tgaaacaaga taaacaaaat
    21601 gaaaaacaaa gtgagatttc taagataaaa caaagcaatg ataataaact tgaaatagct
    21661 caagaattac atgtaagtaa aaatataggg aatatgcttg gatatagaat agataagaaa
    21721 aaaatgaata gaccaagtta atgaaaatgt tgaaaatggt acattttaa
//
LOCUS       DAELXX010000069        21698 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.069, whole genome shotgun sequence.
ACCESSION   DAELXX010000069 DAELXX010000000
VERSION     DAELXX010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21698
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.069"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(88..993)
                     /locus_tag="KPH29_002374"
     CDS             complement(88..993)
                     /locus_tag="KPH29_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P63C domain-containing protein"
                     /protein_id="HBG1467961.1"
                     /translation="MESKEKSILRATHFGIIKIGEKDLNCAVLENGSRIINKSTVFKA
                     FGRTKRGRKKDEIRVPSKPNLPSFIDAKNLSDYISDELEDMLINTIKYKTKNGRIVEG
                     YPAEIIPLICDVYLQARLDNKLAVQQEGLAIASEILVRSLSKIGIVALVDEATGYQYD
                     RDRDELQKILSMYISKEFLPWTKRFPDEFYRQMFRLKNWTYPRPSSKRPGIVGTYTNK
                     YVYDLLPPGVKEELQRVNPTVKPGQRKHKHHQFLTEDIGNDHLKNHLLKVITLMQASK
                     DWKDFNVLFNRAFNLPVQLEIEYDE"
     gene            1904..2119
                     /locus_tag="KPH29_002375"
     CDS             1904..2119
                     /locus_tag="KPH29_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HBG1467962.1"
                     /translation="MDERKLEFSIFCIEALAEKLNIDSEGVYKLLKHDTDILDNYIIP
                     CYEALHSQSKSYIVEDLIQVLEEKGVM"
     gene            2119..2598
                     /locus_tag="KPH29_002376"
     CDS             2119..2598
                     /locus_tag="KPH29_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HBG1467963.1"
                     /translation="MIVYHDSYCVAKTPNIIFLRDSLDFGKGFYVTSIKTQAINWIER
                     FKKRGKKAYLNSYSLDIDKLKENCKVKIFNSYNLEWLDFILECRNMSDVYMKYDVIIG
                     GIADDKIYNTIELYQDNLIEKDEALKRLKYYKPNEQICITNQNVIDKYLKFRCEEEV"
     gene            2604..2834
                     /locus_tag="KPH29_002377"
     CDS             2604..2834
                     /locus_tag="KPH29_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467964.1"
                     /translation="MEANKTLLQILYKKIILEFSNQTGKSLEESMDYLYTSETYKLIS
                     EGVSDMHCKGHIYLAQELMLENGLMYHKGYPR"
     gene            complement(2950..4116)
                     /locus_tag="KPH29_002378"
     CDS             complement(2950..4116)
                     /locus_tag="KPH29_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG1467965.1"
                     /translation="MATKRANGEGSIGKYKDGWRSRIMIGYNEDGKPIRKEFYGKTQK
                     EVKDKLDAYKKQYYLSSDISDDKITLEHWFYNWLFEYRIKDLKPKSFERYESIYRNYI
                     KDTDLGNVKLKDLRVSHMQKYYNRLLDSNKSIPTIRQINTKLKTCLSEAEKQGLIQRN
                     YCTMVNIPVEKKENRLEILTLEQQKNFIKAIEGHKLEVLFLVALGTGLRLGELLGLKW
                     FDIDFKNSNLTVKRTLQRTYFIDKNGNRELKVLEQEPKTSNSYRTVPIPKDVLNKLKE
                     HKVNQNVDILKAGELYRNDNYVFCNELGVPIDDKRPLRNLKSILSSLDIEPIKFHGLR
                     KTYATRLFENDVPPKTVQVLMGHYDISITLNIYTQVMEDKKVEAVEKLDKIFSL"
     gene            complement(4106..4858)
                     /locus_tag="KPH29_002379"
     CDS             complement(4106..4858)
                     /locus_tag="KPH29_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG1467966.1"
                     /translation="MNKSYIPKYTHKFPTNLPHGDKKYTLTYIRRMISEFVYDMGNLE
                     NNPFTFPEVQTLLDGITVGGHKLSDQNQILNIKSSWDYIIKLSNKENLSLNKDTICSI
                     HKIVAKDEALIVGDFRNGNIGIAGTTQYECIEATLLNNLFISDISIINKITNPLEKAI
                     IINLWLSYCQFFYDGNKRTARLTSNLILISNDIGVLSIPAKHKQEYNTLMLNFYETLE
                     ADEVIKFLLEKCITFFDGYNYKSYKELFKNGN"
     gene            complement(4950..5441)
                     /locus_tag="KPH29_002380"
     CDS             complement(4950..5441)
                     /locus_tag="KPH29_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1467967.1"
                     /translation="MINNTIGEKIKQYRKSINMSRNELANKIGCSVHAIAKYEQNQRI
                     PSLDMIRKISTALSIAINKLIVEENDLDTLTNNNINLKVFDKELDLVVHILENYGYDI
                     KTDYKKKFEITDNADTIKPISLSFSDFKDFSKNLNWVINGLVENFIQENSNGLIEDED
                     EEY"
     gene            complement(5438..5635)
                     /locus_tag="KPH29_002381"
     CDS             complement(5438..5635)
                     /locus_tag="KPH29_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467968.1"
                     /translation="MNKAYMLKYTYKYPTNLFYGDKKYTLTYIKRIMSKLVYDIDNLE
                     NNSFICVKIQSLFDDIFKVEL"
     gene            complement(5692..6456)
                     /locus_tag="KPH29_002382"
     CDS             complement(5692..6456)
                     /locus_tag="KPH29_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1467969.1"
                     /translation="MQIGKNLKKIRKQKELTQIQLAEISGISRNALINYENDKRIPSI
                     DTLSKLAKALKIEKTIFYFDIENEVMTNEDVLYFLQSLFPEEYYSFDEFLNAISNLME
                     DDDIRITSSLLLGAWSDKDQYYKLGEKLELNENQVYNWILSDCVSNTELDFSLHPDDI
                     KEIIKNDVLIKQSIDELLDIGLSEENIEAIYDYFSEATDNGSNIRLKKLISQKENMTI
                     KNNDYFKLLQENNELKEKNKKLENTLKEIKKLLTKI"
     gene            6603..6803
                     /locus_tag="KPH29_002383"
     CDS             6603..6803
                     /locus_tag="KPH29_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1467970.1"
                     /translation="MGINLKIFRIKKGLKQQELADRVGISRYYLSNLETGKANNPSND
                     LMIKLSRALDTTVEELFFSKEE"
     gene            6807..7121
                     /locus_tag="KPH29_002384"
     CDS             6807..7121
                     /locus_tag="KPH29_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467971.1"
                     /translation="MKENNNLEVLKTLQATLNIIVKNEEEAQEKKQGYKIIENAKRNK
                     AELITCKEASKLYPIGESKFRQLCHSKNKGFPCIWINNRVYIIKNKLDDWFIENANGI
                     KL"
     gene            7143..7886
                     /locus_tag="KPH29_002385"
     CDS             7143..7886
                     /locus_tag="KPH29_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467972.1"
                     /translation="MSNKGWIKLYRELLDKSIWKDIKPERKVILITLLLMASHKENHV
                     FIDNKRIKIKSGQFISSYSKIAERCGKGVSYQMVRSCLLVFKNAGFLTYETTNKYIVI
                     TIENWELYQENIENQQPNQQTINNQSTTIKNIKNENNIIYSEIISKFNSTCIELSKVK
                     KLTDTRKRRINSIIREYDKEAVLEVINIVSKTSFLNGNNNTGWKANFDWIMNSSNFTK
                     ILEGNYHSKEIVINKETGIYNPDDSQLKW"
     gene            7899..9233
                     /locus_tag="KPH29_002386"
     CDS             7899..9233
                     /locus_tag="KPH29_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG1467973.1"
                     /translation="MNNSNNLIKNTEAEQSILGSILLDSTIVEELEQMLNEDMFYHEE
                     HKYIFNAIKEINKRNEVIDIITLTEELKRKGHLNNIGGISYITSLSTIVPTVSNFTSY
                     VSIVIEKYKNRNIIDKFNKFNLGEIDDNTLIYQLENDINKANQKLIKKDSSITAISER
                     AFEYINDEMNEGIKTGIKFLDDTIGGLYGGELTTIAAKSGRGKTALALQILRNVIFQG
                     KKVLFISGEMSDIQILFRNIASLTGISVVKMKNRNLNDDEIVKYIQALSIINQENNLY
                     INDSVNTVSGIKREIKSTKPSLVIIDYLQILDYEGKEVGEAKFADLSRQIKKITLDFN
                     IPVIQLAQLNDELKDERPKGDRVMRSSKQIYMDSNSVIYIHRPTEKEALNYCKTMGIE
                     DIRELRRREKGFGANLSEIIVAKNRDGESGFRPYWYIGNLFSFEECKKIQEI"
     gene            9483..9755
                     /locus_tag="KPH29_002387"
     CDS             9483..9755
                     /locus_tag="KPH29_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467974.1"
                     /translation="MYDEIIEIYFDYWINEHMYRSILEKDEYYIECWKEVKKINELLE
                     INLEDDKKYLLVELNDLFNSLIEFERELSYKLGFKTGAKFIIELLK"
     gene            10028..11155
                     /locus_tag="KPH29_002388"
     CDS             10028..11155
                     /locus_tag="KPH29_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBG1467975.1"
                     /translation="MIIPKLEPNDRKREFNKYSSEQINKVVYNYLFKNNMSNRKIDDE
                     ILDLSSKQSKGYQSMGIVHHLGINKNHKGIFTGYTIDKAIEVLKEDEIHFKQIINILL
                     EIRNINEYPIIVDSWELLDEKIALKTIDKSTLKHNGTGVPIEIRYFFEINNLGKNEKK
                     NVNFKYNDNYYSAVIEMDKLENPRSRIIWKSKFRDLLRSIFSEYYEKFLENKIEGKTN
                     LPKIRFIKLDSNNFNIEFIFTAEIEDDVLEEISQDNPLNINTINIELETEIRKEGKVK
                     YVYGKQYERNPRNRIEAIKYHGTKCIVCGFDFEKTYGDRGKGYIEIHHIKPLSSVGEE
                     SNINPKTDLVPICSNCHRMIHRKKDNVLSIDDLKQIIKDSK"
     gene            11635..12282
                     /gene="cas5"
                     /locus_tag="KPH29_002389"
     CDS             11635..12282
                     /gene="cas5"
                     /locus_tag="KPH29_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HBG1467976.1"
                     /translation="MILNKIEIIGDMAHFKIPMHSKIQRTYEIPPISTVVGILKNIYG
                     EEIDDFILGYVIYHDGTFKDLQTIYKEINPNVKTLTDSDRFQTDVCPIEYLINPKLII
                     YTNIDKNIEFNEPLCLGKTNCLAKVLSLSEIEVNLIDKKAIGYNQYTDINIGDGMIKR
                     INTLTEYNEKKGYYDIYSSMVRENDEFEYGKYYDEDEQQNIFLWKWKKGGVIKCI"
     gene            12273..13037
                     /gene="cas6"
                     /locus_tag="KPH29_002390"
     CDS             12273..13037
                     /gene="cas6"
                     /locus_tag="KPH29_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBG1467977.1"
                     /translation="MHLVRRHLILTVDNEVVLDYNYQYELMKRIYEAIEINDKRKALS
                     LHNEGYKVDKKVFKLFNYTIMFENAKYLKEGIHLNPQTKIKLILSGYDDILNNIIKGF
                     IKCKVFKLNNIEFKVSDIEEDSKKNFNNITLYKVRSPIVASLYDLKSRKQVYLNPMQE
                     EFYKALHDNLGNKYKLIHNKEYTGELYFDIEDVLAVKKKYITNIKGKGFIIGYTDFEI
                     FVQANKDMQEVIYYCGLGEKNSIGMGLLTYITSRRA"
     gene            13039..14205
                     /locus_tag="KPH29_002391"
     CDS             13039..14205
                     /locus_tag="KPH29_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467978.1"
                     /translation="MDRSYNVFVDNGLYVLAYYLNKDINDITYQDIENSIDLMSDKIE
                     EFVSCEKYSNLKSMCFSNSTLTQPKGKVTLNEKLKGFVKNQGDEYCSLCGQYKAKVKI
                     EDKEYNIGRSYMPNLVANTFYNFSNNLQGLNVCPYCLVLTMYSILNCRVSRYAFLYNS
                     TSSEFMEDYTFHIQKENLTDVELGAKKEKEKYSIVKLLESLVCKYNLFNGDIEQYMFN
                     NSGQSQDIQVNRIKNKYVNLLIKLQEKALLSNFEKLHLDRYILNGTLESNYLREVYRV
                     KKEEKMDEKEQKNFDELVIFLNREVNVLKKVKIDIIKKLTDKLKTSDLDIDKIYKELK
                     MLGKFYAYEDWLVNLNEMYYEKTGEKLFEAEDYIELDDIRSFRQIKNLILVSLI"
     gene            14226..15296
                     /locus_tag="KPH29_002392"
     CDS             14226..15296
                     /locus_tag="KPH29_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HBG1467979.1"
                     /translation="MENKNSNRMLNIGYAIEVLCGRLNGSSSEVEKKNNSYKNSTYTK
                     KIGSRSRISSQCQKYNQKLFMEQVCGYEQNVKTKDGKQVKLVPNPFKYATDDIFGFMM
                     AEKLSIEEDVYKELSDEEKEIYKKNKKKYEVNITKKRKSRWQMNSLINVSNRKVEWEW
                     NVCGTTGDSMPYQVEVYSGVHTNIANLNIGKIGEYDISDIASEHRDYTTLEAKSIGVE
                     KLDKEEKFKRIEALLKSLEYLSVEGNKTNHLTDTKPKFVILGEYSWGNNVFQGIIKEN
                     GLDVEMLKESIEQNEEFRKSDIWIGINKFYDDTFNEIEVNNEKEQIKKYLEEEFEEYD
                     FMKISNVHQAFESYLKYLKETL"
     gene            15307..17292
                     /locus_tag="KPH29_002393"
     CDS             15307..17292
                     /locus_tag="KPH29_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBG1467980.1"
                     /translation="MNNLYEYKAKSDGTSIDKHNEDMNYIMNQLVSIYNISNDMKNDL
                     YEAIVCHDVGKVVKSFQDNIESSTRRVRHELLSASIKYLNRGQKLAILTHHKDIKFLE
                     GQITNSIYEIELKELKELLDIEVEDIRPFIKEISRARNDDTRKLENILLKGYLQYCDH
                     IASAGIKSIDKGFNSIKDFSIPKGVSYNSIQEKVLNMDKNEDIMIMAMTGLGKTATSL
                     YWSNLVQNEYKSKRVYYILPYTASINSLYENLYNRNISVSMLHSKAPYFLNKISDEED
                     IKQLYQIYKKSVKQINVCTIFQLVKSVFSCKRFEMLLSQLKDSIFIVDEIHCFDTRVL
                     CYTLEFLKYLKENLNVKICIMSASIPTCLKKLIQERLQISTIIEPDSKDLIFRHRLNR
                     LSEGILDSIHKIENDLMQGKRVLICVNSVDLSQELYGRLQKYNPKLIHGRFNTRDREQ
                     AEKGIEESFLLIGTQSIEVSLDISYDVLYTELAPLDSLLQRFGRVNRRGDNAELSNIY
                     IYDYDRKLNIYDEDIINRTDIVISDIIHNDNSIVDESKVPSYLDNVYLDIDMDKYNKH
                     KVALEIIMKNMRLGVWNKSANNDMLNNDTISVVPECLLEEYKLYIDKKMYLEASSLFV
                     NVSVKRYNYNKKMFYECGDIVVTSYSYNDKLGLIFDC"
     repeat_region   17545..18167
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=17545..17573
                     /rpt_unit_seq="gtttaaattacactaagttagttataaat"
     gene            18612..20168
                     /locus_tag="KPH29_002394"
                     /pseudo
     CDS             18612..20168
                     /locus_tag="KPH29_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003444896.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            21346..>21698
                     /locus_tag="KPH29_002395"
     CDS             21346..>21698
                     /locus_tag="KPH29_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1467981.1"
                     /translation="MGEKMQSVICKIFDNKLLISNDLSEQDDVLYEVMDKFIDDIEYI
                     SKLGDEQIKSMLMEVNKFEEDSTEFELYMKNLCYLKQSYFIDTNDKSEINSIDIDILK
                     NVKIEDLVDITNIKL"
BASE COUNT         8717 a         2187 c         3314 g         7480 t
ORIGIN      
        1 ctaataagta cttttatgat ataagttaat tctattttta taatataaat taaaagtatt
       61 taaagcgttg ttaaaggcta tagtctatta ttcatcatat tcgatttcga gctgtacagg
      121 tagattgaaa gctctattaa aaagcacatt gaaatctttc cagtcttttg atgcttgcat
      181 taaggttata acctttaata aatgattttt taaatgatca ttacctatat cttctgttaa
      241 aaattgatga tgtttatgtt ttctttgccc aggttttact gtggggttaa ctctttgaag
      301 ttcttcttta actccaggag gtaataaatc ataaacatat ttatttgtgt atgttccaac
      361 tattccaggt ctcttagaac ttggtctagg gtatgtccag ttttttaatc tgaacatttg
      421 tctataaaat tcatcaggaa atctttttgt ccaaggtaaa aactctttag aaatatacat
      481 agacaatatt ttttgcagct catcacgatc tctatcatat tgatatcctg tagcttcatc
      541 aactaatgct acaatcccta ttttggataa acttcttact aaaatttcgc ttgctatagc
      601 tagtccttct tgctgaacag ctaatttatt atcaagcctt gcttgtaaat atacatcaca
      661 tataagaggt attatttcag ctggataacc ttccacaatt cttccgtttt tagtcttata
      721 ttttattgta tttataagca tatcttctaa ttcatctgaa atataatcag atagattctt
      781 tgcatcaata aaacttggta gattaggctt agaaggtaca cgaatttcat ctttttttct
      841 accacgcttt gttcttccaa aagctttgaa tactgtactt ttatttatga ttcttgaacc
      901 attttctagt acagcacaat ttaaatcttt ttcacctatt tttataatac caaaatgtgt
      961 tgctctcaat atactttttt cttttgattc caatttaaca ccatccttta ttttatttta
     1021 ataagtaaga tattacttag tatctcttca ttagataaat tagtatttat aagactaata
     1081 atatcaattt taaaatatgt atatagataa ttgagttttt ttatcatctt gtacgcagta
     1141 tttttattaa caacgatatt ttttgataat tttctatagc taatattttc atcaaatata
     1201 aataaattaa tagcaagtag ccattttctg tagtctagtt tagttttatg cataatggta
     1261 ttagttgtaa ttgaaaactt actattgcaa atattgcaat gaaataaatc attataagta
     1321 ctaattttat cagaataaca ataggggcat attaatgagt tttttaattt aagattatat
     1381 aaaaaaatct tataagagac atctgttgga aagtttttat aaatatcaaa gtcttttgtc
     1441 ataatttaat tatatcatgt tgtagaaatt aatttactgt atttaagtgg gtcaaattta
     1501 atatatatgt ttttgatagt gtaaaaatta aaatataatt ttaggcatgt ttgtattgaa
     1561 taatgagcac tttcaaattt tattagtgta agaggtttca aattataaag taatgaatgt
     1621 atattactat atgatttata attagaataa aatttttata atagaaattt aatatatcaa
     1681 aatatcagtt gaggatattg ttcaagtggt atatcttccc tgagttaaaa aataaatgat
     1741 gaaatatttg gaaatgctag tctttatttg tttatgatat tatgtaacat agaattttaa
     1801 tatctacttt atgatataat atttttataa acataagatg gtttaagtta ttaattaata
     1861 aaaatatata tattatataa gtggatagga gttgaggttt gtaatggatg aaagaaaact
     1921 agaattttct attttttgca ttgaagcttt agcagaaaag ttgaatatag attcagaagg
     1981 agtttataaa cttcttaaac atgatactga tattttagat aactatataa taccttgcta
     2041 tgaagcacta cattctcaaa gtaaaagtta tatagtggaa gatttaatac aagtgcttga
     2101 agaaaagggc gtgatgtaat aattgtatat catgattcat actgtgtggc aaaaacgcct
     2161 aatattatct tcttaagaga ttctttagat tttggaaaag gattctatgt aaccagtata
     2221 aaaacacagg caattaactg gatagaacgt tttaagaaaa ggggtaaaaa agcatattta
     2281 aatagctatt ctctagatat tgataaatta aaagaaaact gcaaggtaaa aatcttcaat
     2341 agttataatt tagaatggct tgactttata ttagaatgta gaaatatgag tgatgtatat
     2401 atgaaatatg atgttataat aggtggaatt gctgatgata agatttacaa tacaatagaa
     2461 ctgtatcaag ataatcttat tgaaaaagat gaagctttaa aaagattaaa atactataag
     2521 ccaaatgaac aaatatgtat aactaatcaa aatgtaatag ataaatattt aaagtttaga
     2581 tgtgaagagg aggtatagtg cttatggaag caaataaaac attattacag atactatata
     2641 aaaaaattat attagaattt tctaatcaga caggcaagag tttggaagaa agtatggatt
     2701 atttatatac ttctgaaaca tataaactta taagtgaggg tgtatcagat atgcactgta
     2761 agggtcatat atacttggct caagaattaa tgctagaaaa tggattaatg tatcataaag
     2821 gctatccaag ataaatagtt aaaactagat acgaattaag gatacaagat aatataaaat
     2881 taatttgtat ccttatttgg gttttgtaat taactatatg aaatcatagt atttttatgt
     2941 atttgatttt tacaatgaaa atattttatc caacttttct acagcttcaa ccttcttatc
     3001 ttccataact tgagtatata tatttaaagt tattgatata tcataatgac ccattaaaac
     3061 ttgtacagtt ttaggaggaa catcattctc aaaaagtcta gttgcataag tttttctaag
     3121 accatgaaac tttataggtt ctatatctaa gctgcttaat atagatttta aattccttaa
     3181 aggtctttta tcatctatag ggacacctaa ttcattgcaa aatacataat tatcatttct
     3241 atataattct ccagccttta atatatccac attttgattt actttatgct ctttcaattt
     3301 atttaaaaca tcttttggaa taggtacagt cctgtaagaa tttgatgtct ttggttcttg
     3361 ctctaatact ttaagctctc tatttccatt tttatctata aaatatgttc tttgaagggt
     3421 tcttttaact gttaaattag aatttttaaa atcaatatca aaccatttaa gtcctaataa
     3481 ttctcctaat ctaagtccag taccaagagc aaccaaaaat aatacttcta atttatgccc
     3541 ctctatagct tttataaaat tcttttgttg ttcaagagtc aatatttcaa gcctattttc
     3601 ctttttctca actggtatat ttaccatagt acaataatta cgttgtataa gaccttgttt
     3661 ttcagcttct gataagcagg ttttcagttt agtgtttatt tgccttatag taggtatact
     3721 tttattggaa tcaagcaatc tattgtaata tttttgcatg tgagatactc ttaagtcctt
     3781 taacttaaca ttgcctaaat cagtatcttt aatataatta cgatagatgc tttcatatct
     3841 ttcaaatgac tttggtttta aatcttttat tctatattca aacaaccaat tgtaaaacca
     3901 atgctctaaa gttattttat catctgaaat atcagaactt aaataatatt gttttttata
     3961 agcatcaagt ttatctttaa cttccttttg agttttacca taaaattctt ttcttattgg
     4021 ttttccatct tcattataac caatcattat acgagaacgc caaccatctt tatattttcc
     4081 gatagagcct tctccattag ctcttttagt tgccattttt aaacaactcc ttatatgatt
     4141 tatagttata tccatcgaag aatgtaatac atttctctaa taagaatttt ataacttcat
     4201 cagcttctag tgtttcatag aagtttagca ttaatgtgtt gtattcttgt ttatgtttgg
     4261 ctggtattga tagaactcct atatcattac ttataagtat taggtttgag gtaagtcgag
     4321 cagttctttt gttaccatca tagaagaatt gacaatagga caaccataaa tttattatta
     4381 tagccttttc taaggggtta gttattttat ttatgatact tatgtcactt ataaataaat
     4441 tatttaatag agtggcttct atgcactcat attgagtagt accagctata cctatgttac
     4501 cgtttctaaa atctccaaca attagagctt catcttttgc aactatttta tgtatagagc
     4561 aaattgtatc tttatttaaa gataagtttt ctttgttact taattttatt atataatccc
     4621 atgatgattt tatatttagt atttgatttt ggtcacttaa tttatgtcca cctactgtta
     4681 taccatcaag taatgtttgt acttctggga atgtgaaggg gttattttct aggttaccca
     4741 tatcatatac aaattcactt atcattcttc taatgtatgt taaagtatac tttttatctc
     4801 catgaggtag attcgtaggg aatttatgtg tgtattttgg tatatatgat ttattcatga
     4861 ttttagctcc tttcatatct aatttttatt tattaactat gaatgtataa aagttaatag
     4921 gtaaacccat attttgaaaa tgtgcaatac taatattctt catcctcatc ttcaataaga
     4981 ccattagagt tttcttgtat aaaattttca acaagaccat ttatcaccca atttagattt
     5041 ttggagaagt ctttgaagtc agagaagctt aagctaattg gttttattgt atctgcatta
     5101 tctgtaattt caaacttttt tttatagtct gtttttatat cataaccata attttctaat
     5161 atgtgaacta ctaaatccaa ctctttatca aatactttta aattaatatt attatttgtt
     5221 aaagtatcta aatcattttc ttcaacaatg agtttattta tggcaattga taaagcagta
     5281 gatatttttc ttatcatatc tagactaggt attctttgat tttgctcata tttagctata
     5341 gcatgaactg aacaaccaat cttgttggct agctcattcc ttgacatatt tatcgatttc
     5401 ctatattgtt taatcttttc tccgattgta ttatttatca taattccacc ttaaatatat
     5461 catcaaacaa tgattgtatt tttacacata taaaggagtt attttctaga ttatctatat
     5521 catatactaa cttactcata attcttttaa tgtatgttag agtatatttt ttatctccat
     5581 aaaataaatt agtagggtat ttatatgtgt attttagcat atatgcttta ttaataattg
     5641 taactccatt tgtagttttt tatcattgct ttagatagta ttgatattat tttatatttt
     5701 agttagtagt ttttttattt ctttaagtgt attttcaagc tttttatttt tttcctttaa
     5761 ttcattattt tcttgtaata atttaaagta atcattattt ttaatagtca tattttcttt
     5821 ttgagatatt aactttttta gtcgaatgtt actcccatta tctgttgcct cactaaagta
     5881 atcatagatt gcttctatat tttcttctga tagaccaata tctaataatt catcaattga
     5941 ttgttttata agaacatcat ttttaataat ttcttttata tcatcaggat gtaatgaaaa
     6001 atctaattca gtattagata cacaatctga aagtatccaa ttataaactt gattttcatt
     6061 tagttctaat ttttcaccaa gtttataata ttggtcttta tcagaccaag cacctagcaa
     6121 taaacttgag gtaatcctaa tatcatcatc ttccataaga tttgaaatag catttaaaaa
     6181 ttcatcaaaa gaatagtatt cttctgggaa taaagactgt aaaaaatata aaacatcttc
     6241 attagtcatt acttcatttt caatatcaaa ataaaagata gtcttttcaa tttttaaagc
     6301 tttagcgagt tttgataagg tatctatact aggtattctt ttatcatttt cataatttat
     6361 tagagcattt cttgagatgc cactaatttc agcaagttga atctgtgtta gttctttttg
     6421 ttttcttatc ttttttaagt tttttccaat ttgcaatatt atcacctcgt ataaagtata
     6481 tcccaaaagt acacaaatga gaacagaaaa ataatatatt cttattgaca gcacacatat
     6541 gtcgacatta taattaaaat gtaaagttta cgaatgtaaa ctagaaaaaa ggaggaaaaa
     6601 gtatgggaat caatttaaaa atatttagga ttaaaaaagg tttaaagcaa caagaactgg
     6661 cagatagagt tggtatatca agatattatt tgtcaaactt ggaaacaggt aaggctaata
     6721 atccaagcaa tgatttgatg attaaactat ctagagcttt agatacaaca gtagaagaat
     6781 tattttttag taaggaggag taaaatatga aagagaataa taatttagaa gttttaaaga
     6841 cattacaagc tactttaaac attattgtaa agaatgaaga agaagcacaa gaaaaaaagc
     6901 aaggatataa aattattgaa aatgcgaaaa gaaataaagc tgaacttata acatgtaaag
     6961 aagcatctaa gctttatcct ataggagaat caaaatttag gcagttatgt cactctaaaa
     7021 ataaaggttt cccttgtata tggataaata atcgagtata cataataaaa aacaaattgg
     7081 atgattggtt tattgaaaat gcaaatggta tcaagttata gtcttaaagt aggtgtttgc
     7141 aattgagcaa taaagggtgg ataaagcttt acagggagtt gttggataaa tcaatttgga
     7201 aagatattaa accagaaaga aaagtaatat taataactct attacttatg gctagtcata
     7261 aagaaaatca tgtttttata gataataaaa ggataaaaat aaagtctgga caatttattt
     7321 cttcttatag caaaatagct gaaagatgtg gtaaaggagt tagttatcaa atggttagaa
     7381 gttgtttgtt ggtttttaaa aatgctggtt ttctaacata cgaaacaaca aacaaatata
     7441 tagtcataac tattgaaaac tgggagcttt accaagagaa catagaaaat caacaaccta
     7501 atcaacagac gattaacaat caatcaacaa caatcaagaa tataaagaat gaaaataata
     7561 ttatatatag tgaaattata agcaaattta actcaacttg tattgaactt tcaaaggtca
     7621 agaaactaac agacacaaga aagaggagaa taaattcaat aataagagaa tatgataaag
     7681 aagctgtatt agaggttata aatatagtta gtaaaacaag ttttctgaat ggaaataata
     7741 atacaggatg gaaagcaaat tttgattgga taatgaattc aagtaatttt accaagatac
     7801 tagagggcaa ttatcatagc aaggaaattg tcataaataa agagacagga atatataatc
     7861 cagatgattc acaattaaag tggtaaggag gttatattat aaataattca aacaacttaa
     7921 ttaaaaatac agaagcagag caatcaatat taggaagcat tttattagac tcaacaatag
     7981 tagaagagtt agagcaaatg ttaaatgaag acatgtttta tcatgaggaa cataaataca
     8041 tatttaatgc tataaaagaa ataaataaga gaaatgaagt aatagatata ataactctta
     8101 cagaagaatt aaagagaaaa ggtcatttga ataatattgg aggtataagc tatattacca
     8161 gtttgtcaac aatagtccca acagtatcaa atttcacgag ttatgtcagt atagttattg
     8221 aaaaatataa aaatagaaat ataattgata aattcaataa gttcaatctt ggagaaatag
     8281 atgataatac tcttatatat cagttagaaa atgatataaa taaagccaat cagaaattaa
     8341 taaaaaagga ttcaagtata acagccatat cagagagagc ctttgaatat ataaatgatg
     8401 aaatgaatga aggtattaag actggaataa agttcttaga tgatactata ggaggtttat
     8461 atggaggtga actaactaca atagcagcca aaagtggaag ggggaaaaca gcattagctt
     8521 tacaaatact tagaaatgta atatttcaag gaaagaaagt actttttata agtggagaaa
     8581 tgagtgatat acagattctg tttagaaata tagcctcact aactggaata tcagttgtaa
     8641 aaatgaaaaa tagaaatcta aatgatgatg aaatagtcaa atatatacaa gctttaagca
     8701 taataaatca agaaaataac ctttatataa atgactctgt aaatacagta tcagggataa
     8761 agagagaaat aaagagtact aagcctagct tagtcataat tgattatttg caaattttgg
     8821 attatgaagg taaagaagta ggagaagcta aatttgctga cttatcaagg caaataaaaa
     8881 agattacttt agattttaat ataccagtta ttcagcttgc tcaattaaat gatgaattga
     8941 aggatgaaag acctaaaggg gatagagtaa tgagaagctc aaagcagata tatatggatt
     9001 ctaattcagt tatatatatt catagaccta cagaaaaaga ggctcttaat tattgcaaga
     9061 caatgggaat tgaggatata agagagttga gacgaagaga aaaaggtttt ggtgctaatt
     9121 taagtgagat tattgttgcc aaaaatagag atggtgagtc aggatttaga ccatattggt
     9181 atataggaaa tttgttttct tttgaagaat gtaagaagat acaagagata taaaaaagaa
     9241 gcatgattta ttaagtaata gataaattat gtttcaagaa gtattgaatg gtaaatatat
     9301 aaaacttaat tagatgtaat agagatttaa aatatattga gtttagattt aaatgcttat
     9361 tagaaaaagg tagttgccat ataaatataa caactacctt tataaattag gtacttaatg
     9421 tacactaacc tagcaactta agtatatatt aagtacctga tgaaagtcaa ggaggtacta
     9481 aaatgtatga tgaaattata gaaatatatt ttgattattg gataaatgaa catatgtata
     9541 gaagtatttt agaaaaagat gagtattata ttgaatgttg gaaagaggtt aaaaaaatta
     9601 atgagttatt ggaaataaat ttggaagatg ataagaaata tctgttggtt gaattaaatg
     9661 atttgtttaa ttcattaata gaatttgaaa gagaacttag ttataaatta ggatttaaaa
     9721 caggagcaaa atttataata gagttgttga agtaattaat ttgtgtatct caatagaata
     9781 attaaatagt aaatcgagat tattaataag ttgtactaga atattttaag tgaagtatat
     9841 attaattata ttgtttaaaa taaatatatt taataaagag gggataagta tgccctcttt
     9901 tatttttatg caatcaatga cattgtagga tttacgtatt ttgataaata gcacaatata
     9961 ttttaataat aaatatatga tacaataatt ttattaaaat ctaaaataat caattgagga
    10021 ggagaatatg ataataccta agttagagcc taatgataga aaaagagagt ttaataaata
    10081 ctcatcagag caaataaaca aagtagttta taattattta tttaaaaata atatgagtaa
    10141 tagaaagata gatgatgaga tactagattt atcatcaaaa caaagtaaag gttatcaatc
    10201 aatgggtata gtgcatcatt taggaataaa taaaaatcat aaaggtattt ttacaggata
    10261 cacaatagat aaagcaattg aagttcttaa ggaagatgag atacatttca agcagattat
    10321 taatatactt ttagaaatta gaaatataaa tgagtatcct ataatagttg actcttggga
    10381 gttattagat gagaaaatag ctttaaaaac aattgataaa tctacattaa aacataatgg
    10441 gacaggagtt ccaatagaaa taagatattt ttttgagata aataatttag gaaagaatga
    10501 aaagaaaaat gtgaatttta aatataatga taattattat agtgcagtta tagagatgga
    10561 taaattagag aatccaagaa gtagaataat ttggaaatct aagtttagag atttactaag
    10621 aagtatattt tctgagtatt atgagaaatt cttagaaaat aaaatagaag gaaaaacaaa
    10681 tttacctaaa ataagattca taaaacttga tagtaataat tttaacatag aattcatttt
    10741 tactgctgaa atagaagacg atgtattaga ggagataagt caagataatc ctttgaacat
    10801 taatactatt aatatagaat tagaaactga aattagaaaa gaagggaaag ttaaatatgt
    10861 ttatgggaaa cagtatgagc gtaaccccag aaatagaata gaagcaataa aatatcatgg
    10921 cacaaagtgt attgtatgtg ggtttgactt cgaaaaaacg tatggagata gaggtaaggg
    10981 atacatagaa attcatcata ttaaaccatt aagttctgtt ggagaagaaa gcaatataaa
    11041 tcccaaaaca gatttggtgc caatatgttc aaattgtcat agaatgattc acaggaagaa
    11101 agataatgtt ttgagtattg atgatttgaa gcaaattata aaagatagta aataatgctt
    11161 atgtgtggca gagatgtata aagtatatat tctaaactag ttaacaacaa atactaatat
    11221 aaaaaatcaa aaaatttatt aggaaaaggt agttgctaaa gtataacagc tacttttata
    11281 aattaagtat ttgaggtata ttagtctaac cgattaggta tatcttaagt tattgatgaa
    11341 aattaattaa gactaacatg tatgatgaat ccatagaaat gtatttttat tgttaaataa
    11401 atgaacatac atagaaatat tttagaaaat tacaagtact atattgaaag tttgaaggag
    11461 aaccttgatt taaggtaagt gtaaagtttt tagaaacttc actaaaataa tctaattaag
    11521 ttatatattt gttactttta ggaaaataat ggtaaaaatt aaaggttttt ataaatgaat
    11581 gtctaatttt ataaataaaa tggagtattt taactaaata tttgaggaga taaaatgata
    11641 ttaaataaaa tcgaaataat tggagatatg gctcacttta aaattcctat gcatagcaag
    11701 atacaaagaa catatgaaat tccaccaata tcaacagttg tcggaatttt aaaaaatata
    11761 tatggtgaag aaatagatga ttttatatta ggatatgtta tatatcatga tggtacattt
    11821 aaagatttac agactatata taaagaaatc aatccaaatg taaaaacatt gacagatagc
    11881 gatagatttc aaactgatgt atgtcccatt gaatacctaa ttaatcctaa acttatcata
    11941 tatactaaca tagataaaaa tatagaattt aatgagccgc tatgtttagg taagactaat
    12001 tgtttagcga aggtattatc tttaagtgaa attgaagtaa atctaataga taaaaaagct
    12061 attggataca atcaatatac agatattaat attggagatg gaatgataaa aagaataaat
    12121 acattgacag agtataatga aaaaaaaggt tattatgata tatattcttc aatggtaaga
    12181 gaaaatgatg agtttgaata tggtaaatat tatgatgaag atgagcaaca gaatattttt
    12241 ttatggaaat ggaaaaaagg gggagtaatt aaatgcattt agtaagaaga catttgatac
    12301 tcacagtgga taatgaagtt gtattagatt acaattacca gtatgagcta atgaaaagga
    12361 tatatgaagc tatagaaatt aatgataaaa gaaaagcact aagtcttcac aatgaaggct
    12421 ataaggtgga taagaaagta tttaaattat ttaattacac aattatgttt gaaaatgcta
    12481 agtatttaaa agagggaata catttaaatc cccaaacaaa aatcaaatta attttaagtg
    12541 gatatgatga tatattgaat aatattataa aaggttttat taaatgtaaa gtatttaaat
    12601 tgaataatat agagtttaaa gtttcagata tagaagaaga ttcaaagaaa aattttaata
    12661 atataacact ttataaagta aggtcaccta ttgtagcaag tttatatgat ttaaaaagta
    12721 ggaaacaagt atacttaaat ccaatgcaag aagagtttta taaagcatta catgataatt
    12781 taggaaataa gtataagctt atacataata aagaatatac tggtgaacta tactttgata
    12841 ttgaggatgt attagcagta aaaaagaaat atataactaa tataaaagga aaaggattta
    12901 taattggata tacagatttt gaaatatttg tacaagctaa taaagatatg caagaagtaa
    12961 tttattattg tggattaggt gaaaaaaata gtataggaat ggggttacta acttatataa
    13021 cttctaggag ggcttaatat ggatagaagt tacaatgtat ttgttgataa tggtctatat
    13081 gtgttggctt attatctgaa caaagatatt aatgatataa cttatcaaga tatagagaat
    13141 agtatagatt taatgagtga taaaattgaa gaatttgtaa gttgcgaaaa atactctaat
    13201 ttaaaaagta tgtgctttag caattctacg ctaactcaac caaaaggaaa agttacttta
    13261 aatgagaagt taaaaggttt tgttaaaaat caaggagatg aatattgtag tttatgtggt
    13321 caatataagg ccaaagtcaa aattgaagat aaagaataca atatagggag aagttatatg
    13381 cctaatttgg ttgcaaatac attttataat ttctccaata atttgcaagg tttgaatgta
    13441 tgtccatatt gtttagtatt gactatgtat tcaattctga attgtagagt aagtaggtat
    13501 gcttttttat acaattctac atcaagcgaa tttatggaag attatacttt ccatattcaa
    13561 aaagaaaatt taactgatgt agagttagga gcaaagaaag aaaaagaaaa gtattcaatt
    13621 gtaaaattat tagaaagtct agtgtgtaaa tataacttat ttaatggtga tatagagcaa
    13681 tatatgttta ataactctgg tcaatctcag gatattcagg taaatagaat aaaaaataaa
    13741 tatgtgaatt tattaataaa attacaggaa aaagcactac tatcaaactt tgaaaagtta
    13801 catttggata ggtatatctt gaatggtact ttagaaagca attatctcag agaagtatat
    13861 agggtaaaaa aggaagaaaa aatggatgag aaagaacaaa agaattttga tgaacttgtt
    13921 atttttttaa acagggaggt aaatgtattg aaaaaagtca aaatagatat aataaaaaaa
    13981 ttaacggata aattaaaaac tagtgactta gatatagaca aaatatataa ggagcttaaa
    14041 atgttaggaa aattttatgc atatgaggac tggttagtta acttaaatga aatgtattat
    14101 gaaaaaactg gagaaaaact atttgaagca gaggattata ttgagttgga tgatataaga
    14161 agctttagac agattaaaaa tttaatatta gtaagtttaa tttaataata ttatggaggg
    14221 ttaaaatgga aaataaaaat tcaaatagaa tgttaaatat aggctatgca atagaagttt
    14281 tatgtgggcg attaaatgga agtagtagtg aagtagaaaa gaaaaataat tcatataaga
    14341 attctactta tactaaaaaa atagggagca gaagtagaat ctcaagtcaa tgtcaaaaat
    14401 acaatcaaaa gctatttatg gaacaagttt gtggttatga acaaaatgtt aaaactaaag
    14461 atgggaaaca agtaaaattg gttccaaacc catttaaata tgcgacagat gatatatttg
    14521 ggtttatgat ggcggaaaaa ttatctatag aagaagatgt atacaaagaa ttatcagatg
    14581 aagaaaaaga aatatataaa aaaaataaaa agaaatatga agtaaatata actaaaaaaa
    14641 gaaagtcaag atggcaaatg aactctttaa taaatgtatc aaatagaaaa gttgaatggg
    14701 agtggaatgt ttgtggaact acaggagata gtatgccata tcaagttgag gtatattcag
    14761 gggtacatac aaatatagca aatcttaata taggaaagat aggagaatat gatatatctg
    14821 atatagcaag tgagcataga gattatacaa ctttagaagc taaatcaatt ggagttgaaa
    14881 agttagataa agaagaaaag tttaaaagaa tagaagctct cctaaaatca ctagaatact
    14941 taagtgtaga aggtaataag acaaatcatc taactgatac aaaacccaaa tttgttatat
    15001 tgggagagta ttcatgggga aataatgtat ttcagggtat tatcaaagaa aatggcttag
    15061 atgtagagat gttaaaagaa tctatagaac aaaatgaaga atttagaaaa agcgatatat
    15121 ggattggaat aaataaattt tatgatgata catttaatga gatagaagtt aataatgaaa
    15181 aggaacaaat aaagaagtat ttagaagaag agtttgaaga gtatgatttt atgaaaatat
    15241 caaatgtaca tcaagctttt gaaagttact taaaatattt aaaggagact ttataaagga
    15301 tatatcatga ataatttata tgaatataaa gctaaatcag atgggacaag tatagataag
    15361 cataatgaag atatgaatta cataatgaat caattagtta gcatatataa tatatctaat
    15421 gatatgaaaa atgatttata tgaggcaatt gtatgtcatg atgtagggaa agtagtaaaa
    15481 agctttcaag ataatattga aagtagcaca agaagagtta gacatgagtt attatcagca
    15541 agtattaagt atttaaatag aggtcagaag ttagctatat taacccacca taaagatata
    15601 aagtttctag agggtcaaat aacaaattct atttatgaaa ttgaattaaa ggaattaaag
    15661 gaactattag atattgaagt agaagatata agaccattta taaaggaaat atcaagagca
    15721 agaaatgatg atactagaaa acttgaaaat atattattaa aaggttactt gcaatattgt
    15781 gaccatatag cttctgcggg aattaaaagt atagataaag gatttaattc tattaaagat
    15841 tttagtatac caaagggtgt aagttataat tcaatacaag aaaaagtttt aaatatggat
    15901 aaaaatgaag atataatgat aatggctatg acaggtcttg gaaaaacagc aacatcattg
    15961 tattggagta acctagttca aaatgaatat aaaagtaaga gagtgtatta tatacttcct
    16021 tatacagcaa gcataaattc tttatatgaa aatttatata atagaaatat aagcgtaagt
    16081 atgttacaca gtaaagctcc atattttttg aataaaatta gtgatgaaga agatataaaa
    16141 caactttacc agatatacaa aaagagtgta aaacaaatta atgtatgtac tatatttcaa
    16201 ttagtaaagt cagtattttc ttgtaagaga tttgaaatgc tactatcaca attaaaagat
    16261 agtatattta tagttgatga aatacattgt tttgatacaa gagtattgtg ttatacattg
    16321 gaatttttaa aatatttaaa agaaaactta aatgtaaaaa tatgtataat gagtgcaagt
    16381 ataccaactt gcttaaaaaa gttaatacag gaaagattgc aaataagtac aattattgaa
    16441 ccagatagta aagatttaat atttagacat agattgaata gattaagtga aggaatactt
    16501 gatagcatac ataaaataga aaatgattta atgcaaggaa aaagagtatt aatatgtgtt
    16561 aattcagtag atttatctca agagttatat ggaaggcttc aaaaatataa tcctaaatta
    16621 atacatggtc gatttaatac tagagataga gagcaggcag aaaaagggat agaagaaagc
    16681 ttcttattga taggtactca aagtatagag gtaagtttag atataagtta tgatgttctt
    16741 tatacagagt tagctccttt agattcattg ttacaaaggt ttggtagagt aaatagaagg
    16801 ggagataatg ctgagttgag caatatttat atatatgatt atgatagaaa gcttaatata
    16861 tatgatgaag atataataaa taggactgat atagtaatat cagatataat acataatgat
    16921 aattctatag ttgatgagag taaagttcca tcttatttag ataatgtcta tttagatata
    16981 gatatggata aatataataa acataaagtt gctttagaga ttataatgaa aaatatgagg
    17041 ttaggggtat ggaataaatc tgctaataat gatatgttaa ataatgatac tattagtgtt
    17101 gtgcctgaat gtcttttaga agagtacaag ttgtatattg ataaaaaaat gtatttagaa
    17161 gcgagttcat tatttgtaaa tgtatctgta aaaagataca attataataa aaagatgttt
    17221 tatgaatgtg gagatatagt tgttacaagt tatagttata atgataaact aggtctaata
    17281 tttgattgtt aagagaatta tagtataact aattaaaata ttttattgag aatttgttat
    17341 aatttatatc gactacaaat tttatatgtt acaatagaat aattttgcag tgagcgaaaa
    17401 atatgtgtaa atggctgtga gttagtgatg gcaaggttta taactgaatt tttgttttta
    17461 gctaaaaaca ctactggtgc ctcactgcaa attaactatt ttaataaccc tacaagtgtt
    17521 gtaattacta tagttttatt tggggtttaa attacactaa gttagttata aatattccgg
    17581 aaagtacttt tgctatttga ttcattctac ccgtttaaat tacactaagt tagttataaa
    17641 tttcactaat tttagttaca actattacaa gtccagccgt ttaaattaca ctaagttagt
    17701 tataaattat gtgtaatgta ttttaatgtt ttctagcaac aatgtttaaa ttacactaaa
    17761 ttagttataa attttataaa ttctatgaat aattttaaag agaggtttcg tttaaattac
    17821 actaagttag ttataaattg tacatttgac atagaagctt caaaatccat cccagtttaa
    17881 attacactaa gttagttata aatgtaacag cacgaatgat ggcacagacc tatctgcaat
    17941 gtttaaatta cactaagtta gttataaata gaagtacact tacaagtaag ataaaagacc
    18001 caggtacagt ttaaattaca ctaagttagt tataaattct gtagctatat gtggaagtaa
    18061 ttttagtaga gaggtttaaa ttacactaag ttagttataa attattgaag gggtgagagt
    18121 tgtgactagg cactacaagt ttagattaca ctaaattagt tgtaaattta cttattcatt
    18181 aagaaactta actgagagtt tgatttatat tattgaatta gcaagagttt tagaaagtag
    18241 aaaattatac cccaataata ccccaaccac aaaaataaat aataaaaaat atccaaaaaa
    18301 attagtataa catcataact aaatagctat atacctacac ttacgaaaac caacaaaaca
    18361 atcaaataac cacttgtaga ctcccctcgt ctccaccaat tttgaaaatg aaaataatag
    18421 aataaaagga attacttatg tttggactaa gtaattcctt ttattttgct taaaagcaca
    18481 caattagcac acaactagaa ggttatataa ataaaaaggc tgtcgcacta gaaaatagtg
    18541 ctacgctctt ttttgtataa aaaaataaat cccaagttca aaagaacctg ggattcttgt
    18601 ataataactg tatgctaaaa caaaatacta cacaaattga ttatactact tatggtgata
    18661 attatcaatt taagctacca ctaaatattg attacatgat tccaaaagat gattctgtaa
    18721 ggttattaag ttaaattatt gaggagatga atttagaaaa attatataag acttattctc
    18781 gaattagatg aaatagagta atccatagac aatttttgaa agttataata tatacagata
    18841 tgaactgtat ttattcttcg agagaaatag aaagaacatg caaaagagat attaatttta
    18901 tgtaccttct aggcgaaacc tccactccag accattcaac aattgcaaaa tttcgcagta
    18961 tacattttta actaataggc aaaagcttga ttgcacagtt tacaaacttt cttggagata
    19021 ataatgaaat ttcaaaagac acaatattta ttgatggtac aaaaatagag tcatctgcta
    19081 atagatatac ttttgtttgg aaaaaagctg taaataaaaa tatggctaaa atggtgaata
    19141 atatatctga cttcatattg aaatgtgaga ttgatgttgg cattaaaata gtttatgaag
    19201 ataaaattaa aatctatcat ttaaagaaac taaaaaaaat atcaaaagaa gcaaatgtag
    19261 catttattca tggaaaagga aatagaaaat ctactaaaaa ccttcatata atgggtgaac
    19321 gaaatagttg ttctaaaaca gataacgatg aaacttttat gagaatgaaa gaagatcata
    19381 tgaaaaatgg gcaactgaaa ccagcatata atatacaatt tggtgtagat taagaatata
    19441 tagtttggct aaattcagga aatcaaactg ctgatactac aacattaata ccatttttaa
    19501 aatctataga ggaatatact aagtttaaat atcctaaagt aatagctgat ggtggatatg
    19561 aaagtgaata aaactatgca tttttagagc ataataatca gttatctttc ataaaacctg
    19621 caaattatga aattattaaa actagaaaat ataaaactta tattggatgt attgacaata
    19681 tggattatga agaagaaaat aattattata tttgtacaaa tgagaaaaaa ctatttgcta
    19741 ataaaataat aaataggaaa tcaaaaacag gatataaaag tgaaataaca tgttacattt
    19801 gtgaagagtg tagtaattgt caatataaaa gcaattgtat taagagaagt aatagtaagg
    19861 ttcctcttaa aaataggact aaaaacttac aagtatctaa gtcgtttcat gaaaaaagaa
    19921 aagaaaactt aaagagaatt atgagcctcg agggggatga acttagagtg aatagaagta
    19981 tacaagcaga aggagctttc gctcaagtaa aataaaatat gaaacttaca agatttttaa
    20041 gccaaggtaa agaaaatgtt ttataaggat atattattgt ggcgatagct tataacgttt
    20101 ctagactaca caataaaata gtaaaaaata aacgtggaat atatctttat catttaaaaa
    20161 aatattctaa aattattatt tataatcaaa tattatctga aaaaatggta tttttatatc
    20221 tttatttgtc atgcataatt ttacttagaa cagaaacttt atataaaaca attgaaaata
    20281 tacctattaa gataattaga aaagagctct cacagaacaa atttaatttg ctcatgcaac
    20341 agccccatat tgatatacat ttaggttgta taattataca tttcaatatt acatgttcat
    20401 taggataata gttagtaaat gatatgattg agagaataga tataataaac taatctagtc
    20461 ctgtattgca atgtttatta aagaatatat ttttaaagat ccaagtactg catctcttcc
    20521 agaattgagt tggttaatag ttttttgatg ctatgcaatt tataataaaa agtataatga
    20581 tttagttgat gaatcatcag aatttatacc ctcaaagatt aatcagattg caataaaaat
    20641 aatgatatat tctatagtta taatatctat tttctttgca acccctcata caatgaatat
    20701 aaatatatca aacttggata tgggtataat tctcatttca gttttattta tacagttttt
    20761 atctaaatta attttgtatg tatattttga taaaagggag atatgtaact aagtggtaat
    20821 attggtgaaa acaaaaatta aagaatatag aaaaaaactc cttatgacac aaaatgaact
    20881 tgctaaatca gttagggtaa ggtgtgagac tattgtacac ttggaaaata taatccttct
    20941 ttaaagttag tgatagatat tgctaaagta ttcaatacta cagtagaaaa tttgtctgaa
    21001 tttattgaag aaggatagtg gatagattat aaatagttat aagatggtta atgaaatata
    21061 gttacttaat tattaagtgt gttagaaata tttatatgta acacaatgtg tagttaagat
    21121 taccttatct catctaagac tttaattttt cttcccataa agagggtata tcaattttct
    21181 agtgtggtag cccatttttt attgatttaa atttataata tatattaata gatgatactt
    21241 aaaaatttta taaaatatct aagttgtcat aaataaaacg atatatctaa ccgaatatgg
    21301 taaaatttgt tataatatag ataagttaga gtaaaaataa ttttaatggg ggagaaaatg
    21361 cagtcagtaa tatgtaaaat ttttgataat aaattactaa ttagtaatga tttaagtgag
    21421 caggatgatg tattatatga ggtaatggat aaatttatag atgatattga atatatttca
    21481 aaattaggag atgagcaaat taaaagtatg cttatggagg taaataaatt tgaagaagat
    21541 tcaactgaat ttgaactata tatgaaaaac ttatgctatt taaagcaatc atattttata
    21601 gatactaatg ataaaagtga aataaattct atagatatag atatattaaa aaatgttaaa
    21661 atagaggact tagttgatat aactaatatt aaattaga
//
LOCUS       DAELXX010000070        20812 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.070, whole genome shotgun sequence.
ACCESSION   DAELXX010000070 DAELXX010000000
VERSION     DAELXX010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20812
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.070"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            358..669
                     /gene="rpsJ"
                     /locus_tag="KPH29_002396"
     CDS             358..669
                     /gene="rpsJ"
                     /locus_tag="KPH29_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBG1467982.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            761..1390
                     /gene="rplC"
                     /locus_tag="KPH29_002397"
     CDS             761..1390
                     /gene="rplC"
                     /locus_tag="KPH29_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBG1467983.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1420..2043
                     /gene="rplD"
                     /locus_tag="KPH29_002398"
     CDS             1420..2043
                     /gene="rplD"
                     /locus_tag="KPH29_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBG1467984.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2043..2333
                     /gene="rplW"
                     /locus_tag="KPH29_002399"
     CDS             2043..2333
                     /gene="rplW"
                     /locus_tag="KPH29_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBG1467985.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTASFKKAVVKLTADSKEIEFFQGM"
     gene            2363..3193
                     /gene="rplB"
                     /locus_tag="KPH29_002400"
     CDS             2363..3193
                     /gene="rplB"
                     /locus_tag="KPH29_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBG1467986.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3228..3509
                     /gene="rpsS"
                     /locus_tag="KPH29_002401"
     CDS             3228..3509
                     /gene="rpsS"
                     /locus_tag="KPH29_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBG1467987.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            3541..3876
                     /gene="rplV"
                     /locus_tag="KPH29_002402"
     CDS             3541..3876
                     /gene="rplV"
                     /locus_tag="KPH29_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBG1467988.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            3899..4714
                     /gene="rpsC"
                     /locus_tag="KPH29_002403"
     CDS             3899..4714
                     /gene="rpsC"
                     /locus_tag="KPH29_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBG1467989.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            4750..5181
                     /gene="rplP"
                     /locus_tag="KPH29_002404"
     CDS             4750..5181
                     /gene="rplP"
                     /locus_tag="KPH29_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBG1467990.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5183..5386
                     /gene="rpmC"
                     /locus_tag="KPH29_002405"
     CDS             5183..5386
                     /gene="rpmC"
                     /locus_tag="KPH29_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBG1467991.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5410..5664
                     /gene="rpsQ"
                     /locus_tag="KPH29_002406"
     CDS             5410..5664
                     /gene="rpsQ"
                     /locus_tag="KPH29_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBG1467992.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            5690..6058
                     /gene="rplN"
                     /locus_tag="KPH29_002407"
     CDS             5690..6058
                     /gene="rplN"
                     /locus_tag="KPH29_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBG1467993.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6082..6387
                     /gene="rplX"
                     /locus_tag="KPH29_002408"
     CDS             6082..6387
                     /gene="rplX"
                     /locus_tag="KPH29_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBG1467994.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6417..6959
                     /gene="rplE"
                     /locus_tag="KPH29_002409"
     CDS             6417..6959
                     /gene="rplE"
                     /locus_tag="KPH29_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBG1467995.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            6977..7162
                     /locus_tag="KPH29_002410"
     CDS             6977..7162
                     /locus_tag="KPH29_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBG1467996.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7195..7593
                     /gene="rpsH"
                     /locus_tag="KPH29_002411"
     CDS             7195..7593
                     /gene="rpsH"
                     /locus_tag="KPH29_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBG1467997.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            7624..8166
                     /gene="rplF"
                     /locus_tag="KPH29_002412"
     CDS             7624..8166
                     /gene="rplF"
                     /locus_tag="KPH29_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBG1467998.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8202..8570
                     /gene="rplR"
                     /locus_tag="KPH29_002413"
     CDS             8202..8570
                     /gene="rplR"
                     /locus_tag="KPH29_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBG1467999.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            8591..9100
                     /gene="rpsE"
                     /locus_tag="KPH29_002414"
     CDS             8591..9100
                     /gene="rpsE"
                     /locus_tag="KPH29_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBG1468000.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9115..9300
                     /gene="rpmD"
                     /locus_tag="KPH29_002415"
     CDS             9115..9300
                     /gene="rpmD"
                     /locus_tag="KPH29_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBG1468001.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9333..9776
                     /gene="rplO"
                     /locus_tag="KPH29_002416"
     CDS             9333..9776
                     /gene="rplO"
                     /locus_tag="KPH29_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBG1468002.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            9820..11088
                     /gene="secY"
                     /locus_tag="KPH29_002417"
     CDS             9820..11088
                     /gene="secY"
                     /locus_tag="KPH29_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBG1468003.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11105..11755
                     /locus_tag="KPH29_002418"
     CDS             11105..11755
                     /locus_tag="KPH29_002418"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBG1468004.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            11756..12502
                     /gene="map"
                     /locus_tag="KPH29_002419"
     CDS             11756..12502
                     /gene="map"
                     /locus_tag="KPH29_002419"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBG1468005.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            12524..12793
                     /locus_tag="KPH29_002420"
     CDS             12524..12793
                     /locus_tag="KPH29_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HBG1468006.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYDFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            12831..13049
                     /gene="infA"
                     /locus_tag="KPH29_002421"
     CDS             12831..13049
                     /gene="infA"
                     /locus_tag="KPH29_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBG1468007.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13085..13198
                     /gene="rpmJ"
                     /locus_tag="KPH29_002422"
     CDS             13085..13198
                     /gene="rpmJ"
                     /locus_tag="KPH29_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBG1468008.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13351..13722
                     /gene="rpsM"
                     /locus_tag="KPH29_002423"
     CDS             13351..13722
                     /gene="rpsM"
                     /locus_tag="KPH29_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBG1468009.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            13758..14156
                     /gene="rpsK"
                     /locus_tag="KPH29_002424"
     CDS             13758..14156
                     /gene="rpsK"
                     /locus_tag="KPH29_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBG1468010.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14188..14811
                     /gene="rpsD"
                     /locus_tag="KPH29_002425"
     CDS             14188..14811
                     /gene="rpsD"
                     /locus_tag="KPH29_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBG1468011.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            14889..15836
                     /locus_tag="KPH29_002426"
     CDS             14889..15836
                     /locus_tag="KPH29_002426"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBG1468012.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            15857..16198
                     /gene="rplQ"
                     /locus_tag="KPH29_002427"
     CDS             15857..16198
                     /gene="rplQ"
                     /locus_tag="KPH29_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBG1468013.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16318..17151
                     /locus_tag="KPH29_002428"
     CDS             16318..17151
                     /locus_tag="KPH29_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBG1468014.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17139..18005
                     /locus_tag="KPH29_002429"
     CDS             17139..18005
                     /locus_tag="KPH29_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBG1468015.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDISFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            17999..18802
                     /locus_tag="KPH29_002430"
     CDS             17999..18802
                     /locus_tag="KPH29_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBG1468016.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            18834..19565
                     /gene="truA"
                     /locus_tag="KPH29_002431"
     CDS             18834..19565
                     /gene="truA"
                     /locus_tag="KPH29_002431"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBG1468017.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            19685..20116
                     /gene="rplM"
                     /locus_tag="KPH29_002432"
     CDS             19685..20116
                     /gene="rplM"
                     /locus_tag="KPH29_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBG1468018.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20145..20537
                     /gene="rpsI"
                     /locus_tag="KPH29_002433"
     CDS             20145..20537
                     /gene="rpsI"
                     /locus_tag="KPH29_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBG1468019.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
BASE COUNT         8312 a         2447 c         4331 g         5722 t
ORIGIN      
        1 atggatttaa tataaaaaaa agaggggggt aactcctctc ttttttgttt tttgttgaca
       61 agtagcgcta aagcatatat actaaactag tgccttgaga cagattaaca atcaagttta
      121 aggttatgat gttgtaatag aaaataaaat tgaaaaaaac agaaaaaaaa cttgaaaaaa
      181 tgtaacagat gatataaaat ataatagtgt gcttgagaga ttaagaaaat aataattaaa
      241 tcctagtgaa atacgaactg atatcgattt aaaagttaca acacacccgg agcagtgtat
      301 cggtattggt tgtacgcaac ttgaaaatta aacacgtgat aaaggaggga aaacaaaatg
      361 gctaagaatg aaaaaataag aataagatta aaatcatatg atcacaaatt attagatttt
      421 tcagcaggta aaatagttga aactgctaaa aaggctggat cacaagtttc aggacctgtg
      481 cctctaccaa cagaaaaaca agttgtaact atattaagag ctgtacataa gtacaaagac
      541 tctagagaac aatttgaaat aagaactcat aaaagactaa tcgacatagc taatccaaca
      601 cctaagacag ttgactcatt aatgagatta gacttaccag caggtgtaga tatagaaata
      661 aaattataag aacccctaag atattatcct atagatgatt atcttagaat gattactcta
      721 aaagatgtaa tccgctgtaa gaattatagg aggtgctaat atgaaaggaa tattaggcaa
      781 aaaagttggt atgactcaaa tattcactga taaaggtgta gttatacctg taacagctgt
      841 tgaagcaggg cctatggtcg ttactcaaat aaaaacagtt gataaagatg gatacaatgc
      901 tatacaaata ggttttgaag atgctaaaga aaaagcttta aacaaaccta aaaaaggaca
      961 tttagctgct gctaatgttt taaagaaaca tttaaaagaa tttagagtag attctgtaga
     1021 aggatacaca gtaggacaag aaataaaagc tgatgtattt gaagcaggtg caaaaataga
     1081 tgttactgga ataagtaaag gtaagggatt ccaaggtcca ataaaaagac atggacaaag
     1141 tagaggtcct gaaactcatg gttctagata ccacagaaga ccaggttcaa tgggagcttg
     1201 ttcttaccca ggtagagtat ttaaaaacaa aaaattagct ggacatatgg gaagcgtaaa
     1261 ggtaacagtt caaaacttag aggttgttaa agtagatgct gacaagaacc ttatattagt
     1321 taaaggagct ataccaggag ctaaaggttc agtagtaact ataaaagaag ctataaaggt
     1381 ttctaaataa tgactaacct agagaaagga ggaataacaa tgccaaaatt aaatgtacta
     1441 aatgttagtg gacaaaacgt tggagaaata gaattatcag attctatatt tggcgtagaa
     1501 gttaatggac atgttttata cgaagttgta aaaaatcaat tagcaaataa gagacaagga
     1561 actcaatctg ctaaaactag agcagaagtt agaggtggcg gaagaaaacc ttggaaacaa
     1621 aaaggaacag gtagagcaag acaaggttct acaagatctg tacaatgggt aggtggagga
     1681 gttgcttttg cacctaaacc aagaagctac aaatatacat tacctaagaa ggtaagaaga
     1741 ttagctatga agagtgcttt atcttcaaaa gttcaaaata gtgaagttat agtattagat
     1801 gcattaaaca tggatgctcc taagactaaa gaatttgctc aaatattaaa caatataaat
     1861 gctgctaaaa aagctttagt agttatagct gacaaaaacg ataacgtaat aaaatcagct
     1921 agaaatatag aaggtgttca aactgcatta gttaatacta tgaatgtata tgatatatta
     1981 aaatacgatt catttataat aactacagat gctgttaaaa aagtggagga ggtgtacgca
     2041 taatgactaa tccacatgat gtaataataa gaccagttgt aactgagcac agtatggctg
     2101 aaatgggtga aaaaaaatac acttttgttg tggctaaaga tgcgaacaaa actgaaataa
     2161 aaaaagcagt agaaaaagta ttcggagtta gtgttgataa agtaaatact ttaaactacg
     2221 atggaaaagt taaaagaatg ggtagaactc aaggaagaac agcaagcttt aagaaagcag
     2281 tagttaaatt aactgctgat agtaaagaaa tagaattctt ccaaggaatg tagtattata
     2341 acaaacgaag gagggaaaac agatggctat aaaaaagttt agaccaactt ctcctgcctt
     2401 aagacaaatg acagttttag tttctgatga aataacatgt aatcaaccag aaaaatctct
     2461 tttagttaac ttaaagaaaa atgctggtag aaacgtacat ggtagaataa ctgttcgtca
     2521 tagaggtgga ggacaaaaaa gaaaatatag aataatagat tttaaaagag ataaagatgg
     2581 tatacctgct aaggttgcta ctatagaata tgatccaaac agaactgcaa atatcgcatt
     2641 attaaactat gctgatggag aaaaaagata tatattagct ccagttggaa ttaacgttgg
     2701 agatactata ttatcaggat taggtgctga tataaaacca ggaaactgtt tagcattaaa
     2761 agatatgcca gttggtacta tcattcataa tatagaatta aagccaggaa aaggagctca
     2821 attagttaga tctgctggag tttctgcaca attaatggct aaagaaggaa aaaatgcttt
     2881 attaagatta ccatcaggtg agatgagatt agtaagcata aactgtaaag ctactatagg
     2941 tcaagttgga aatatagagc atggtaacgt agttatcggt aaagcaggta gaaaaagaca
     3001 tatgggaata agacctactg taagaggttc tgtaatgaac cctaatgacc atccacacgg
     3061 tggaggggaa ggtagatctc caataggtag accatctcct gttactccat ggggtaaacc
     3121 agctcttgga tacaaaacta gaaagaaaaa taaagcttca aataaactaa tagtatcaag
     3181 aagaactaag tagtaaattt atttttgctc ggaaaggagg aatataaatg tcaagatcaa
     3241 ctaaaaaagg gccttttgtc catgcaagac ttttaaagaa gatagaagct atgaatgcta
     3301 gtggaaataa agaagttata aaaacttggt caagatcttc aactgtattt ccacaaatgg
     3361 tagaaaacac tatagctgtt catgatggaa gaaaacatgt tccagtatat ataacagaag
     3421 atatggttgg acataaatta ggtgagttcg ttcctactag aactttcaaa ggacataagg
     3481 atgacgaaaa atctaataag agaaaataat taaattatga ctaagaaagg aggaataaca
     3541 atggaagcaa aagctactgc taaatacgta cgtgtatcac caagaaaagc aggccaaata
     3601 tgtgaccttg ttagaggaaa gaatgttgat gaagcattag caatattaaa gtttactcca
     3661 agaggagcag cttcaataat agctaaggtt gtaaaatcag ctaaagctaa cgcagaaaac
     3721 aatcacgaaa tggatactga aaaattatat atagcatcaa tagttgctaa tcaaggacca
     3781 acaatgaaga gattcatgcc tagagctatg ggtcgtgcaa ctacaataag aaaaagaact
     3841 tctcatatag aggttgttgt taaagaaaaa aaataattaa tagataggag ggaaagtaat
     3901 gggtcaaaag gttaatccac acggcttaag ggtcggtgtt ataaaagatt gggactcaag
     3961 atggttcgct actgataaaa aagagttcgg aaacttatta ttagaagacc ataatatacg
     4021 taaattctta aagaaaagat tatactcagc tggagttgct aagatagaaa tagaaagatc
     4081 agctaacaaa ataaaaatgg acttacacgt tgctaagcct ggtgtagtta taggaagagc
     4141 tggtgcagga atcgaagcat taaaagctga attagaaaaa atgacaaaga aaactataat
     4201 agttaatata gtagaagtaa gaagtacaga taaaaatgct caattagtag ctgaaaatat
     4261 agctttagct atagagagaa gggttgcttt cagaagagca atgaaacaag ctatacaaag
     4321 agcaatgaaa tctggtgcta aagggataaa agtttctgct tcaggaagat taggtggagc
     4381 tgaaatggct agaactgaag gttatagtga aggaaatgtg ccactacaaa ctttaagagc
     4441 agatatagat tatggtttcg ctgaagctga tacaacttat ggaaaaatcg gtataaaagt
     4501 ttggatatgt aatggtgaag ttttaccaac tagagacggt gtaaatccaa gagaagaaag
     4561 cagaaagagc gatagaagag acaacaagag agataataga agaaacgata gaagaggaaa
     4621 tgacagaaga ggaaatgata acagaggaaa ctacagagga caaagacctc aaggtggatc
     4681 aagacctcaa agaactgaaa acaaaggaaa ttaataatag ttaggttcaa ggaaggagga
     4741 aaaatactca tgttaatgcc aaaaagagta aagcgtcgta gagttcatag aggaagcatg
     4801 gctgggcaag ctcataaggg taataaagtt acttatggtg agtttggatt agtagcatta
     4861 gaagcttctt ggataacttc taatcaaata gaagctgcca gaatcgcgat gactagatat
     4921 ataaaaagag ggggaaaggt ttggataaaa atattccctc ataaaccagt aacaagaaaa
     4981 ccagcagaga ctcgtatggg tgcagggaaa ggttctccag aatattgggt agctgtagtt
     5041 aagccaggaa gagttatgtt tgaattagca ggtgtttctg aagataaagc aagagaagct
     5101 atgagacttg ctgcacataa attaccaatc aaatgtaaat ttgttaaaaa agaagattta
     5161 gaagtaaagg gtggtgaata ggatgaaagc taaagaatta agagatttaa caagcgaaga
     5221 gctaatgaac aaattaaacg acttcaaaag tgaattattt agcttaagat tccaattagc
     5281 tactggtcaa ttagaaaata cagctagaat aaagtttgtt aagaaggata tagcaaaagt
     5341 taaaactgtt cttgctgaaa gaaagttata cgaaactaga gcttaatttg gaaaggaggc
     5401 tttttaaaca tggaaagagg aagaagaaaa gttagaatag gccgtgttgt tagtgataag
     5461 atggataaaa caatagtagt agctgttgaa gaattcgtac gtcatccact atataataaa
     5521 cgtgttaaga gaactaagaa attcaaggct catgatgaaa agaatatatg taacatcgga
     5581 gatagagtta aaataatgga aactagacca ttatctaaag ataagagatt cagactagtt
     5641 gaagttgttg agaaagttaa gtagtttttg aagaaggagg gattacgata tgatacaaca
     5701 agaatcacgt ctaagagttg ctgataactc aggagctaaa gaacttttat gtatacgtgt
     5761 tctaggcgga agtaaaagaa gatatggtaa cataggcgac gttatagttg ctactgttaa
     5821 aagtgcaaca cctggtggag ttgtaaaaaa aggtaaggtt gttaaagctg ttatagtaag
     5881 aagcaaacaa ggcgtaagac gtaatgacgg aagctatata tcttttgatg aaaatgctgc
     5941 tgttataata aaggacgata aaactccagt aggaactcgt atattcgggc ctgttgctag
     6001 agagttaaga gacaatgaat tcatgaaaat agtttctctt gctccagaag tactataatt
     6061 aaggaggtgc aataggacat gatgcgtgtt aaaaaaggtg acactgttgt agttatagca
     6121 ggtaaagata aaggtaaaaa aggttcagtt ttaaaggtat atcctaaaac tagcaaagtg
     6181 ttagttgaag gtgtaaatgt aataactaaa catcaaaaac caagtgctat gaaccaacaa
     6241 ggtggaataa taaataaaga agccccaata cacatatcta atgtaatgcc atttgatcct
     6301 gaaacaggaa aaggcgttag agtaagatat gaagtaaaag atgggaacaa agtaagagta
     6361 tcagcaaaga gcggaaaaga attataataa atattgcttg aaaggaggga ccttaaatgg
     6421 cttctagatt acaagaaaaa tacatgaaag aagttgctcc tgctttaatg gagaaatttg
     6481 gatacaaaaa cgtaatggag atacctaagt taaataaaat agttattaac atgggtatag
     6541 gtgacgctag agaaaatcca aaaggattag aaaaaggcgt tgaagaatta gaaatgatat
     6601 caggacaaaa gcctgttata actaaagcta gaaaatctgt tgctaacttc aaattaagag
     6661 aaggaatgcc aataggaaca aaagttacat taagagctga caaaatgttc tactttatgg
     6721 acaaattagt ttcagtttct ttaccaagag ttagagattt tagaggagtt aaccctaatg
     6781 cttttgatgg tagaggaaat tatgctttag gagttaaaga acaattaata ttccctgaaa
     6841 tagaatatga taaaatagat aaagtaagag gaatggatat aatatttgtt actacagcaa
     6901 aaactgacga agaagctcgt gaattattaa aattattagg aatgccattt tctaagtaag
     6961 caaaggaggg attcccgtgg ctagaaaagc gatggttgtt aagcaacaaa gaaagcaaaa
     7021 gtacgctact agagaataca ctagatgtac tatatgcgga agaccgcatt cagttttgaa
     7081 aaaatttggt atatgccgta tatgctttag agaattagct tataaaggtc aaatacctgg
     7141 tgttagaaaa gcaagctggt agaagcttga ttagaatgga aggagggcaa caaaatgaca
     7201 atgacagatc caattgcaga tatgcttact cgtataagaa atgctaatgt tgttaagcat
     7261 gaaactgtag atgttccagc ttctaatatg aagaaagaat tagctagaat cttattagaa
     7321 gaaggtttca taagaggtta tgatgttata gaagatggaa aacaaggtat cataagaata
     7381 caattaaagt acggacaaga aggcgaaaga gttataacag gtcttaagaa aatatctaag
     7441 cctggaatga gagtttacgc tgctaaccat gagataccaa aagtattaaa cggattagga
     7501 atatcagtta tatctacttc aaaaggaata ttaactgaca agcaagctag aaaagaaaat
     7561 gttggtggag aagtaatctg ctacgtttgg taattaatat gaacaatgta aggaggtgcg
     7621 accatgtcaa gaataggtgt taagccaata ataataccag caggtgttga agttactata
     7681 gctgaaggaa atttagttac agtaaaaggt ccaaaaggaa ctttaactaa acaattaagt
     7741 gctgagttaa acataaaaaa agaagaaaat acaataatgg ttgaaagacc aactgataat
     7801 aaaaaacata gatctttaca cggactaact agaactttat tagataatat ggttgtaggt
     7861 gtaaatactg gtttcgaaaa gaaattagaa ttaaaaggtg ttggatatag agctcaaaaa
     7921 caaggaaaga aattagttat gaacttaggt ttctctcatc cagttgagat ggaagatcca
     7981 gaaggaataa ctgttgaagc tccaaaccaa acagagttaa tagtaaaagg tatagacaag
     8041 caattagtag ggaattatgc tgctaagata agagcttgga gaaagccaga gccatacaaa
     8101 ggtaaaggta taaaatacgt tgatgaagta atcagacgta aagaaggaaa aactggtaaa
     8161 aaataatacc aaaacttaaa cgctagaaag gagtgagcta ggtgttaaaa aaggctgata
     8221 aaaatgctaa tagacttcaa agacataaga gagttcgtag aaaaataagc ggtacttctc
     8281 aaagaccaag attatgtgta tttagaagtg ctaacaatat atatgctcaa ataatagatg
     8341 acacaaaaag agtaactcta gttgctgcat cttctttaga agcagaagtt aagagtgctg
     8401 tcaatcatac aggaaacaag gaagcagcta agaaagttgg agaattagtt gctaaaaaag
     8461 ctgttgaaaa aggaatcact gaagttgtat ttgacagagg tggatactta tatcatggaa
     8521 gaattcaaga attagctgaa ggtgctagag aagctggtct taagttctaa tacgaaggag
     8581 ggaaaaatac atgctacgtc gtaagccgat agatgcagga caacttgact tacaagaaaa
     8641 agttgttgaa gtaagacgtg ttactaaggt tgtaaaaggt ggtagaaatt tcagatttgc
     8701 agcattagta gttgttggag atgaaaacgg acacgttggt ataggtgctg ggaaagcaat
     8761 ggaagtacca gacgctataa aaaaagcagt tgaagatgct aagaaaaatc taatagtagt
     8821 gccaatagtt gggactacaa ttcctcacga agtacgcgga cactttggtg ctggaaatat
     8881 attaataatg cctgctgtag aaggtacagg agtaatagct gggggacctg ctagagccgt
     8941 acttgagcta gctggactaa aagacgttag agctaaatct ttagggtcta acaatccaag
     9001 aaatatggta aatgctacaa tagaaggact taattctcta aaaacagttg aagatatagc
     9061 taaacttaga ggtaaaaaag ttgaagaact tctagggtaa ggaggtagtg taaaatggct
     9121 aaattacaaa taaagttagt tagaagtgtt ataggaacta ctcctaacca aaaaaagaat
     9181 gttgaagcgt taggattaag aaaaagagaa caagttgttg taaaagaaga caatgctcaa
     9241 acaagaggta tgataaataa agtaagtcac ttattagaag taactgaaat agctgaataa
     9301 tttttataaa gtagtgcaag gaggtgcaat ccatgaagtt acatgagtta aaacctgctg
     9361 aaggtgcagt aagagctaag agaagattag gtagaggtac tgcaactgga caaggtaaaa
     9421 ctgcaggacg tggacaaaaa ggtcaatggt ctcgttctgg tggtggagta agagtaggat
     9481 ttgaaggtgg acaaatgcca ctagctagaa gacttcctaa gagaggtttc aataacatat
     9541 ttaagaaagt ttacacagaa gttaatgttg aagttttaaa cagatttgaa aatggaacag
     9601 aaataactgc agaattatta aaatctacta aaactataag caaaataggt aaagacggaa
     9661 ttaaaatctt aggtgaaggt aatttagaga aagctttaac tgttaaagct gctaaattta
     9721 cagcttcagc tcaagaaaaa atagaaaaag ctggtggaaa agcagagtta gtataataac
     9781 ctgctaatct cctagccagt acaggcgggg tgaattagcg tgctgtcaaa attaaaacaa
     9841 gcttggaaga ttaaagcagt aagaaaaaaa gtaatgtata cacttatgat gatcgtgata
     9901 ttcagaatag gtactactat accagttcca ggcatagaca ctagtattat acaaaagatg
     9961 gtaggcggca atagtctact ttctctgtat aatatgttta ctggtggagc atttagtaac
    10021 ttttcactat ttgctttagg tataagtcca tatatcactg catcaatcat aatacaactt
    10081 ctaacagtag gttttgaaag tttagcagaa cttcaaaaat ctggtgaaga aggaaagaaa
    10141 aaaatcaata agtacactaa atatacagca ctagctctag cagttgtaca agctttggga
    10201 attacattag gaattgtaag aagtgcttta atatcaaata gtgtgttttt tatcacaact
    10261 gttgtaataa cattagtttc agcaagtatg ttagtaatgt ggattgggga caaaattact
    10321 gaaaaaggaa tcggtaatgg tagttcagta ataatatttg caggtataat atcaagaata
    10381 cctacagatg ttattaaaat atcgcaacaa gttaaatctg gcgaagttgc accttgggtt
    10441 atagtgattc tagctgtagt tatattactt acagtaacag gagttacatt tatacaagaa
    10501 gcaactagaa agatacctgt acaatatgct aaaagagttg ttggaagaaa aatgtatgga
    10561 ggacaaagtt ctcatatacc aatgaaagtt aaccagtcag gagttatgcc tataattttt
    10621 gctagttcac ttttagcttt ccctcaaaca atagctatgt ttatgggtcc aaatgctcag
    10681 gcttttgttc aaaaatactt aagtatggca acagaacaag gattttggac atatagatca
    10741 attgagattc tacttataat tttcttctca tatttttata caactgtatc atttaataca
    10801 gaagacatat ctaaaaatat gaaaaataat ggtggattta taccaggtat aagaccaggt
    10861 gagcctacta tggattattt aaatagaatt ttaactagat taactttagc tggagctact
    10921 ttcttagcaa taattgctat ggtgccagct cttacaacac attatatgaa agtaaatatg
    10981 agtctagctg ggacttcatt gctaatcgtt gttggagttg ctttggagct taagagacag
    11041 ttagagtcaa acttagttat gagaagctat caaggtttct taaaataaaa tggagatgga
    11101 taatatgaga ataatattac ttggacctcc aggtgctggt aaaggtactc aggcggcagg
    11161 aatagtagaa aaatacaata tacctcatat atcaacagga gatatattca gaaagaatat
    11221 aaaagaggga acagaacttg gaaaaaaagc taaagaatac atggaccaag gtttattagt
    11281 accagatgag ttaactgtag gtttagttac tgatagaata tctcaagaag attgtaaaaa
    11341 tggatttatg ttagatggat ttccaagaaa tgtagcacaa ggagaacatt tagatatctt
    11401 cttaaaaaat gctggtatat cactagataa agttgtcaat attgaagttg ataagagtat
    11461 attagtgtct agagcagttg gtagaagaat atgtaagtct tgtggagcta cttaccatgt
    11521 tgagtttaat cctcctaaag tagaaggtgt atgtgatgta tgccaaggag aattatatca
    11581 aagagctgat gataatgaag aaactgtatc taagagaata caagtttatc tagatgaaac
    11641 taagccttta gtagattatt atagcaaaca aggtataata gcagatataa aaggtgatca
    11701 agcaatagat aaagtatttg aagatattgt cgcagcctta ggaagtggaa aataaatgat
    11761 tatcttgaaa tcaaaaaaag aaatagaact tttaagagaa gcaggtaaaa tagttgctga
    11821 cactcatgaa gttttaagaa aagctatttc tccgggaata tctactttag aattagataa
    11881 aatagctgaa gagaatataa gaaaatacaa tgcagagcct tcttttaaag gatatggagg
    11941 ttttccagga agtatatgtg cttctatcaa tagagaggta gtacatggta ttccaggaga
    12001 aactatatta caagaaggtg acattgtaag tttggatata ggagcctatt ataaaggata
    12061 tcatggtgat tctgcgaaaa ctcacggagt aggcatgata tctgaagaag ataggaaatt
    12121 gatagaagta actagagaaa gcttctatga aggaataaaa tttgctaaat taggatatag
    12181 actttcagat atctcacacg cagtacaaac acacgttgag aaacatggtt tctcggtagt
    12241 tagagattta gtgggtcatg gagttggagc aaatcttcat gaagacccac aagtacctaa
    12301 ttatggactc ccaggaaaag gaccaagact tagagaagga atggttattg ctatagagcc
    12361 aatggttaat gcaggtcgtt atcatgtaaa gactctatca gatgggtgga cgactgttac
    12421 aatagatgga aaaaaatcag ctcattatga gcatacgata gcaattactg aacatgaacc
    12481 tttgatattg acaaagttgt aatttaaaaa gtaggtgaaa aaagtgctat cagataattt
    12541 aagtataggt caagttgtta aagtttcatt aggacgagat aaaggaaatt tattctttgt
    12601 agtcaaaata attaataatg aatatgtatt aatagctgat ggaaaaaaga gaaaacttga
    12661 caaacctaag ttaaagaaag tgaagcactt aaaaaaatat gattttatta atgatgaagt
    12721 tagaaaaaga gtagtatctg gacaagagat tacagattct tttttaagag ctgaacttac
    12781 taagttaaat tagataattt aacttattga aatggagggc tggttagtta atggccaaaa
    12841 aagatgttat agaattagaa ggtacagttt cagaagcttt acctaatgct atgttcaaag
    12901 ttaaactaga aaatggacat gagatattat gccacatttc tggaaagcta agaatgaact
    12961 tcataagaat tcttgaaggt gacaaggtga atgttgaact ttctccatat gaccttacaa
    13021 gaggaagaat tacttggcgt aagaagtaga ctatattaat atttagtcta aggagggatt
    13081 aataatgaag gttagaccat cagtaaaacc aatatgtgaa aaatgtaaag taataaaaag
    13141 aaaaggaaaa gtaatggtta tctgtgaaaa tccaaagcat aagcaaaagc aaggataaga
    13201 aattattact agcattttgt taaattttat agtataatac tatattgtga tgttaacaaa
    13261 agaacgttaa aatcgttgaa caatctggcg taggattggc atatgatgtg aagttttaaa
    13321 cactaaaaac tatgtgtagg aggtgcgatc atggcaagaa tagctggggt agatttacct
    13381 agagaaaaaa gagcggagat aggcttaact tatatatatg gtataggtaa agcaactgct
    13441 aacgaaatat tagctaaggc tgagataaat ccagatacta gaataaaaga tttatcagaa
    13501 gatcaagtta atgagttaag aaaagttata gatgacgatt tcttagttga aggtgactta
    13561 agaagagaaa ttgctttaaa cataaagaga ttaagagata taaaatgtta tagaggaata
    13621 agacacgcta aaggtcttcc attaagagga caaagaacta agactaatgc tagaactaga
    13681 aaaggtccta gaaaaacagt atctcgtaaa aagaaaaaat aaataaataa taactagatg
    13741 aaggagggaa aaggaaaatg gctaaaccaa aaaagaaagt tacacgtatt agaagaagag
    13801 aacgtaaaaa tatagaacgt ggtcatgctc atatacaatc aactttcaat aatacaataa
    13861 taactttaac tgacgttcac ggaaatgcta tatcttgggc aagttctgga caattaggat
    13921 tcaaaggatc aagaaaatca actccatttg catctcaaat ggctgctgaa acagctgcaa
    13981 aagctgcgat ggaacacgga ctaaaaagtg ttgaggtatt cgtaaagggg ccaggttcag
    14041 gaagagaagc tgcaataaga gctttacaag caactggact agaagtaact atgataaaag
    14101 acgttactcc aatcccacat aacggatgca gaccaccaaa gagaagaaga gtgtaattaa
    14161 taagagtaca ataggaggtg tagataaatg gcaagatata caggtgcatc atgtagacaa
    14221 tgccgtagag agggaatgaa attattcctt aaaggtgata gatgttatac agacaaatgt
    14281 gctatagtaa aaagaaacta tgctccaggt caacatggcc aaggaagaaa gaaagtttct
    14341 aactatggat tacaattaag agaaaaacaa aaagttaaaa gaatatatgg agttttagag
    14401 actcaattta gaaatttata tgaacgtgct gaaaatatgc ctggtaaagc aggtgagaac
    14461 ttattaagtt tattagagag aagattagat aatgtagttt acagaatggg actagcatct
    14521 tctagaaaag aagctagaca attagtgact catggtcatt tcactttaaa tggaaataaa
    14581 gtagacatac cttcattaat agttaaggtt ggagatgtta tagaagttaa agagaaatca
    14641 agatcttctg caaaattcaa aaatttagta gaagttaact caagaatagc tcctaagtgg
    14701 ttagaagcta atgtagaagg aatgacagca aaagtagttg gtgttccaac aagagaagat
    14761 atagatcttg agatagcaga gcacttaatc atagaacttt actctaaata ataagcacac
    14821 ttttaaaaat tattttaaaa ttttgtttac cctcagtgga ttactgaatt ttaaggaggg
    14881 ttttgtccat gatagaaata gaaaagccaa aagtagatat agttgagctt agcgaagact
    14941 atagatatgg taagtttgtt atagagcctc tagaaagagg atatggaata actataggta
    15001 acgcattaag aagaatatta ttatcatcat taccaggtgt agcagtgaat gctataaaaa
    15061 tagatggagt tcttcatgaa ttttcaacaa tacctggtgt taaagaagat gttactgaga
    15121 taatattaac tttaaaagag ctttcagcaa ctatagatgg tgaaggaagt agaacactta
    15181 aaatagaagc tcaaggacca tgctctatca caggtgcaga tataatctgt cctccagatg
    15241 ttgaaatatt aagtaaagat ttagcaatag ctacattaga tgataatgct aaacttaata
    15301 tggagatatt tgtagataaa ggtagaggtt atgtttctgc tgaagaaaat aaaacagaga
    15361 atgttccaat aggtgtttta cctgtggatt caatatatac tcctgttgag aaggttagtt
    15421 atcatgtgga aaacacaaga gttggtcaaa aaacagatta tgataaatta gtactagaag
    15481 tttggactaa tggtagtata aatcctcaag agggaatatc attagctgca aaggtattag
    15541 ttgagcattt aaacttattt atagacttaa ctgaacatgt aagtagtgtt gaaatcatgg
    15601 tagaaaaaga agaagatcaa aaagaaaaag ttcttgaaat gactatagaa gaattagatt
    15661 tatcagttag atcatataac tgtttaaaga gagcgggaat taatacagtt gaagaattag
    15721 ctaataaatc tgaggatgat atgatgaagg ttagaaacct aggtaagaag tcattagaag
    15781 aggtaataca aaaactagaa gaacttggat tgggattaaa accaagtgaa gaatagcgag
    15841 caaaggaggg ataagaatgg ctaagtaccg taaattagga cgtgaaacag ctcacagaaa
    15901 ccttatgtta agaaacttag taacttgctt actaagaagt ggaagaatag aaactacagt
    15961 gactagagcg aaagaaactc gtagaatggc tgaaaagatg ataactcttg ctaagagagg
    16021 agatcttcat gctagaagac aagttttagc ttatgttatg gatgaaacag tagttaataa
    16081 cttattcaca gatttagctc caaaatatgc tgagagaaat ggtggataca ctagaataat
    16141 aaaaataggg ccaagaaaag gcgatgctgc tgaaatggct tttatagaat tagtatagta
    16201 aggaaaggat taggcttgat ggcttaatct tttttttatt tttttgacta aggtatgata
    16261 taataatgtg taaaatgatt tttataagtt attgcttact aaaaggagta tgagttaatg
    16321 gataatatag taaaggtaaa taatatttcg tttgagtata ttacagatga agcaaaactt
    16381 aaagcaatag ataatttaag cctagatgtt aaaaaaggag aatttgttgc gataatagga
    16441 cataatggtt ctggtaagtc tactttatct aaaaacttaa atgctattct tatgcctact
    16501 gaaggaaata ttcttattga tgatatggat actaaagaag aagaaagatt atgggatata
    16561 agacaaactg caggcatggt atttcaaaat ccagataatc aaatagttgc tactatagta
    16621 gaagaagacg ttgcatttgg acctgagaac ttaggtatag aaccaaaaga aataagacga
    16681 attgtagagg agtctttaaa aagtgtaggt atgtatgatt taagagatag acagcctcat
    16741 ttgttatcag gaggtcaaaa gcaaagagtt gcaatagcag gaattattgc tatgaggcct
    16801 aaatgtataa tatttgatga agcaacagca atgttagatc catctggaag aaaagaagtt
    16861 atgaaaacaa taaaaagact taataaggaa gaaaatataa ctgttataca tataacgcac
    16921 tttatggaag aagcagtaga agctgataga gttgtagtta tggaaaaagg aaagaaaatc
    16981 ttagagggaa ctcctagaga agtattcagt aaaataaaga tgttaaagga aatcggttta
    17041 gatgtacctt gtatgacaga gttatctagt ttattaatag aagaaggtat aaatattagt
    17101 agtgatatat taactgtgga tgagatggtg atggaattat gtcaattata gtaaagaatt
    17161 taacacatat atataatgaa gggatgcctt ttgcaagtaa ggcacttgat gatatttctt
    17221 ttgagattaa agatagagat tttgttggtc ttataggtca tacaggctca ggaaaatcta
    17281 cattaataca acatttaaat ggattactaa agccttcctc aggagaaata tttataaatg
    17341 attttaatat aacagataaa aacttgaatt taactgagat tagaaaaaga gtaggggttg
    17401 tatttcaata tccagaatac caattgtttg aagaaacgat tgataaagat atagcttttg
    17461 gaccctctaa tttaggctta gaggagtctg agatacataa cagggtaaaa gcatcaatgg
    17521 aagctgtagg acttgattat gagggattta aagacaaatc tccttttgaa ttatctgggg
    17581 gacaaaagcg tagagtagca attgctggag ttatagccat gaacccagaa gttctcatat
    17641 tggatgaacc aactgctggt cttgaccctg gtggaagaga tgaaattttt aacttaataa
    17701 aagacttaca tgaaaaaaag aatatgacta taatactatc atcacatagt atggatgata
    17761 tggcaaagtt agcaaaaact ttaattgtta tgaatcatgg tagcgtagaa tttatgggaa
    17821 ctcctagaga agtgtttaaa tcaaatgcta gtaaattaaa agatatagga ttagatattc
    17881 cacaagtttt agagcttgca ttgaaattga gagaaaaagg ctttgacatc agtgaggata
    17941 tattgacttt agaagaagca aaacaggaaa tattaaaagt tgtgagagga cgaggattat
    18001 gttaaaagat ataactatag ggcaatacta cccaacaagt tctgctattc ataaattaga
    18061 tccaaggata aagcttgtag cgacaattgt atttatggta tctatatttg tagtaaataa
    18121 attttggcct tatatagtag ttttattatg tttattagct atgataaaat tggctaatat
    18181 accagtcaaa tatattgtta agggtgttaa acctcttaaa tggattattt tgtttacatt
    18241 tcttataaat atttttttct taccaggtga tgaaatatgg tcatttggat ttttagctat
    18301 tacaaaacag ggtctaagac aagcaatttt tatggcaata agattaatat ttttagtagt
    18361 tggtacatca ttgcttactt taacaacttc accaatagaa ttaactgatg gaatagagag
    18421 attgttgaat ccatttaaga aaattggttt gccagttcat gaacttgcaa tgatgatgac
    18481 aattgcttta cgttttattc ctacattgtt agatgagaca gacaaaataa tgaaggctca
    18541 gatgtctaga ggtgcagatt ttgaaagtaa gaatcttata aatagagcaa agaatttagt
    18601 accacttttg gtacctttat ttgtaagtgc atttagaaga gctgatgaat tggctatggc
    18661 catggaggcc agatgttaca gaggtggaca taataggact aagatgagag agtctgttat
    18721 atcaagaagg gattatatgg catgtgtatt tcaggttgtt tatctgggag caattatagc
    18781 tactagattt atagcaattt aattaataag ttatagatat aatgaggtaa agtatgagaa
    18841 atataaaaat taaaatccaa tacaatggta agaattattg tggatggcaa aagcaacctg
    18901 attctttagg gatacaaggt acaattgaaa gggctatata tgatataaca aaagaagaaa
    18961 ccagtcttat agggtctggt agaactgatt caggagttca tgctattggt cagattgcaa
    19021 attttaaaat taattcaggt ataagtatag aaagtatacc aatggcacta aatgccaagt
    19081 tgccaaaaga catatcagtt atagaagcat gtgaagtaaa tgatgatttt cactccagat
    19141 acagtgcaaa aggtaaaaca tataaatatt tagtatataa tagcaagttt agaaatccaa
    19201 tacttagtga gatatcatat caagtaaaat atgaacttga ttttgataaa atgtgttcag
    19261 aagcaaaaag tttattagga acacatgatt ttaaaggttt tatgagttct ggttcatctg
    19321 taaaagatac agttagaacg atatatgata ttgatataag taaaaaagat gacttgatta
    19381 cttttgagat tagtggtaat ggttttttat ataacatggt gaggattatt gttggaacac
    19441 ttgttgatat gggtcgtggc agaattaacg aaccattttt agatataata caatcaaaaa
    19501 ctaggtcaag atgtggacat acagctccag cacaaggatt atttctcaaa aaagttcatt
    19561 attaacttga cacaccaggc tacatgtatt aaaatatatg agaatgtgtt aataagtcca
    19621 cttgccccgg acttgacata aacggtaaaa aaattttatg aaaagtagtt aaggagggac
    19681 agttatgaaa agttatatag ctaagccagc tgatgtacaa agaaaatggt acttagttga
    19741 tgctgaagga aaaacattag gtcgtttagc aacagaaatt gcgacagtat tgagaggtaa
    19801 acataaacca acatttactc ctcacgttga tggtggagat tttgttgttg tagtaaatgc
    19861 agaaaaaata gttttaagtg gtaagaaatt agatcaaaaa tattacagat accatactgg
    19921 ttatgtaggt ggattaaaag aaatatctta cagagatatg atggataaga aaccagaaga
    19981 agttatatca catgctgtaa gcggtatgtt acctaaaaat aaattaagaa gcagaatgat
    20041 gactagatta agagtatttg caggtgctga gcacactcat gcagctcaaa atccagaagt
    20101 tttaaacttt aaataagatt aataggtgaa aggaggagtt tataatggct aacgttcaat
    20161 attatggaac tggaagaaga aaaagttctg ttgctagagt aagactagtt gcaggtgaag
    20221 gaaatatatt agtaaatgga agagcattag aaaattattt taattatgag acattaataa
    20281 gagatgttaa acaaccatta gttttaactg gtaatgaaaa taaatatgat gttatagtaa
    20341 aagttgaagg tggaggattc actggacaag ctggagctat aagacatgga atatctagag
    20401 ctttattaaa agctgattta gatttaagac ctgctttaaa gaaagaaggt ttcttaacta
    20461 gagatgcaag aatgaaggaa agaaagaaat acggattaaa agcagcaaga agagctccac
    20521 aattctcaaa aagataatta cttatttttt tggaaaagca aaatccttta tttactgtta
    20581 ttcagaaagt attggtattt taaaaatgtt gggttaagtt aattattgat atataataaa
    20641 ataataagat atcttattag gatgtttttc attaagaaga acatcctttt tattttgact
    20701 atttaataaa aaacatatgc taataacatt taaattttat ttttataata ttgtaattta
    20761 aaagtttata taaggttata tattttaaga tattatttaa caatataaat tc
//
LOCUS       DAELXX010000071        20174 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.071, whole genome shotgun sequence.
ACCESSION   DAELXX010000071 DAELXX010000000
VERSION     DAELXX010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20174
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.071"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..535)
                     /locus_tag="KPH29_002434"
     CDS             complement(<1..535)
                     /locus_tag="KPH29_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468020.1"
                     /translation="MSNQIKDFDITNDMFQESIPITTKTILEDMPSNDELNHVFSKGC
                     ERKMKKRKIVLITLLLIGVLLLGSILYNLFLVKAANISMLKESWNFDIPIPNKEIEVF
                     DTQDSINGDGQSYFIQGFSEKNFKKVFNLKGGIVVSKDNINEIEKYIDKFKRDSVNIN
                     KSNKNKIEEDFKKYKLEV"
     gene            complement(945..1121)
                     /locus_tag="KPH29_002435"
     CDS             complement(945..1121)
                     /locus_tag="KPH29_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468021.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(1175..1271)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2067..2468
                     /locus_tag="KPH29_002436"
     CDS             2067..2468
                     /locus_tag="KPH29_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HBG1468022.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGCLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLMSIALKPLAQPPFPLERIIEKLEKEETE"
     gene            2465..3532
                     /gene="rsgA"
                     /locus_tag="KPH29_002437"
     CDS             2465..3532
                     /gene="rsgA"
                     /locus_tag="KPH29_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBG1468023.1"
                     /translation="MNGMKQINLQDYGLNRNFITQSMEYHDLYLARIISQEKNFYQAV
                     CVNGVLFAEVSGKLRFEATIQSDFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRK
                     AAGKVNEEQVVAANIDIVFLCMSLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCD
                     NVHRKLQVVNSIALGVDVVVTSSIEPDGYKQISKYIKSGCTVALLGSSGVGKSSLINC
                     LLGGEFLNTKEIRIDDDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKS
                     FADIEAYFGKCHFRNCTHTSEPGCAIWEAIAHQELSPKRWESYRRLKTEAVFADNKSE
                     YLAKKEKKFKNISKINKTIRR"
     gene            4068..4766
                     /locus_tag="KPH29_002438"
     CDS             4068..4766
                     /locus_tag="KPH29_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468024.1"
                     /translation="MKISSQYRSQYSFRHESNINNTRINESMVKKNETVGKDTYLSNI
                     MKQKQELNDRIRDLKYRQEVYTKKINEAIKNLYKSEIRETTNNFSNIEIGIKNSIIED
                     KNKSTMLDENSTYLNTNDEKESLITKESNEKIEEEILNDEKLEELEQKKDYKEDSNKK
                     EKVSEDLSLVGKTREELENMLKNFINLTQEEIMKLESRIEKLDKDAEEYKQNSKTNIF
                     DKTDEQKKHINVLI"
     gene            4868..5416
                     /locus_tag="KPH29_002439"
     CDS             4868..5416
                     /locus_tag="KPH29_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468025.1"
                     /translation="MEGLGFYAAALSGSSYQRIGFGKLDPIEVIADGDWISYKQAQDT
                     LTVIRNFLNSFDWRNASEMERANRAAKLVTEAKYVDSKYCNIVYGNLVDKRGVCGSFA
                     SSFHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRSDGSEISAFGGALDFDYRKLK
                     DATREMTTYYDAKALSILGFNQ"
     gene            complement(5817..6773)
                     /locus_tag="KPH29_002440"
     CDS             complement(5817..6773)
                     /locus_tag="KPH29_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG1468026.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDNNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVTDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSYGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIQFSNILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENKSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            7476..8471
                     /locus_tag="KPH29_002441"
     CDS             7476..8471
                     /locus_tag="KPH29_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG1468027.1"
                     /translation="MSIIELNELTKSYRRFEKKAGFMGSVKSLFNRKYTEKTAISSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNHFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKTSVIVLKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            8449..9312
                     /locus_tag="KPH29_002442"
     CDS             8449..9312
                     /locus_tag="KPH29_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBG1468028.1"
                     /translation="MSIMSNYNEASSNYSMFYKFKQSIRRYYEVAKINFKVQLIWRFD
                     VVVNMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSE
                     EICNRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFT
                     SNPVQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFFRMIIENIVEFITGTMIPL
                     VLLPETIVTVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYK
                     RMRTLFDGVGI"
     gene            9309..10103
                     /locus_tag="KPH29_002443"
     CDS             9309..10103
                     /locus_tag="KPH29_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBG1468029.1"
                     /translation="MMKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLM
                     FNSIYSHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYIT
                     KPLDPLFRLTFENINIGSSPLILASILIVLYGISKLNSEITLVMIIIYIFMVLLMTLL
                     FYDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGVFKVLFYLILPYGIMS
                     TIPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            10388..11767
                     /locus_tag="KPH29_002444"
     CDS             10388..11767
                     /locus_tag="KPH29_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG1468030.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTVSGMVFFSKNKELLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTIFCIC
                     KYKNRYKYM"
     gene            12222..12935
                     /locus_tag="KPH29_002445"
     CDS             12222..12935
                     /locus_tag="KPH29_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1468031.1"
                     /translation="MDTYFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIAFRVKAQLRRQQYQSIVPSDSAVIKIGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(13207..14745)
                     /locus_tag="KPH29_002446"
     CDS             complement(13207..14745)
                     /locus_tag="KPH29_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG1468032.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQKSAISENEQYLIKASDYQEQLIASNFYSIDNEINKIANGLGLDSIGMNRVVEEL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSNYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
     gene            15628..16083
                     /locus_tag="KPH29_002447"
     CDS             15628..16083
                     /locus_tag="KPH29_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBG1468033.1"
                     /translation="MDEIDLKIINSLKKNSRASTSEISRQINLSVPAVSERIRKIEQS
                     NLIQKYTIQINREKTKYKLLAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDY
                     LIKVLAEDTSNLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            16132..16716
                     /locus_tag="KPH29_002448"
     CDS             16132..16716
                     /locus_tag="KPH29_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HBG1468034.1"
                     /translation="MKVLVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSREQMDEVKLAMGFKL"
     gene            complement(16721..17044)
                     /locus_tag="KPH29_002449"
     CDS             complement(16721..17044)
                     /locus_tag="KPH29_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1468035.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLESL"
     gene            17196..17714
                     /locus_tag="KPH29_002450"
     CDS             17196..17714
                     /locus_tag="KPH29_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG1468036.1"
                     /translation="MNFVELTKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGRQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            complement(17890..18558)
                     /locus_tag="KPH29_002451"
     CDS             complement(17890..18558)
                     /locus_tag="KPH29_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468037.1"
                     /translation="MLEISHKVKEIYPNIKFGVMVINITHSKPNKENFLVLKNSTIKN
                     IIEQHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKMELPLKIELANGSEHFYGIGGKEHILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKVNS
                     IDIF"
     gene            18673..20088
                     /locus_tag="KPH29_002452"
     CDS             18673..20088
                     /locus_tag="KPH29_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG1468038.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNDTFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNSKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELSPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQYVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIENILEYDHEIILGYAHLS
                     FDEIEKGVKILSKIIHMNQGT"
BASE COUNT         7382 a         2325 c         3061 g         7406 t
ORIGIN      
        1 taacttctaa cttatatttt ttaaaatctt cttcaatttt atttttatta cttttattaa
       61 tgttaacaga atctctttta aatttatcaa tatatttttc tatttcatta atattatctt
      121 tgcttacaac tataccacct tttaaattaa aaactttttt gaaattcttt tcagaaaatc
      181 cttgaataaa ataactttgt ccatctccat taatactatc ttgagtatca aatacttcaa
      241 tttctttgtt aggtattggt atatcaaaat tccaagattc ctttagcatt gaaatattag
      301 ctgcttttac taaaaaaaga ttatatagga tacttcctaa taacaaaaca cctattaata
      361 gtagtgtgat taatacaatt tttctctttt tcatttttcg ttcacaacct tttgaaaaaa
      421 catgatttaa ttcatcattt gaaggcatgt cttcaagaat agtttttgtt gtgataggaa
      481 tgctttcttg aaacatatca tttgtaatat caaaatcttt tatttgatta ctcaatatcc
      541 atttcttcta atatttcttt tgattttttc tagtacattg aatactctta tatacattct
      601 cctcttttaa aattaaaagt tataaaagaa taagttatat aaaaaattat tatatttttt
      661 aatatagctt atttgcaaat ttataatttt gttaatctaa tccttcaaac taaaaaatca
      721 tttatgttta cagaatttca ttttaagaga tgtttatttt cacatagata agagaattta
      781 agttctcata tttattattt gatacaaatt tacatcttta ttatatgtat tttaaataga
      841 aaataaaatt atgaatacta gaaatctatc ttaattagct ttctaacata aggctattta
      901 aagtagataa aactaaaaag ggttctctag aaaaccaaat ctatttaatt atacttactt
      961 actaatacca tcacttgatg caaacaagat tctaccacat tttctccgta ctctagtata
     1021 aaatcatcta gttgtgtatc tataaaatca caataatcat atgaactacc aatataagca
     1081 gtcaacatat gtgtacatct atccatttta tctctaaaca tatatattat tatcccccta
     1141 aaaaactttc ataaaaacaa aaagaatatc tctttttggc aactggctga cataactcat
     1201 agtttatttt ataagcgtta gtccctatag ctttgcgtca ctaaatttct ctagttttgc
     1261 cgatttagtt ttattctaca attacaataa tacaatagaa ttgcttatta ttcaatatac
     1321 ttatttgaaa attaaataaa tttacaaata tgaacgttat tttatataaa aaaaatagac
     1381 tattcatgta gaatatatgg aaaaacttga ttgtaggttt tgtatgaaaa gacagttgtt
     1441 attaatttaa gtgagataaa ttgagcaata ggcataaaat gagaaaacaa ataatagcaa
     1501 ttaattttta ttattaatac taggtgaaca ttaaatttta aaataccact agattttata
     1561 gaataagaaa atgagacaaa cataaatata tgaatatatt aaattatact tgaacatact
     1621 tatattttat gataaaattt taataataat tattagtgaa ggagtgtctt gcctgatgtc
     1681 agagttatat ataatggtaa ccaacaaata aatagttgga gacgcatatg ggttctgttt
     1741 aatcagaatt tttaagtgta tttttgatac atttgattgt tgtgtcttac tctgccttta
     1801 tagataacta acattagaca acaaataata atattacata ttactttgtt aaattgtggt
     1861 gtagtgggct ttaatctgct acacttttat taattttaca cagtattatt ttgaatataa
     1921 ttatgttgaa atagaagata ctaaattttc tagtccacag gggttttctg tttttgtgta
     1981 ggtaaatatg tataccacat aaaaaggcag atataaatac ctgtggactt tttttgtaga
     2041 aaatacaaga tacggaggaa ataaaaatga attataaaaa tagaaaacga gcttattatc
     2101 aggaaaattc ctgtatggat aactttattt gcaaaaattg tggttgcctt gtagttcctg
     2161 aaggagctgg tacacaacat agaaatcatt gtccacattg tttacatagt cttcatgtag
     2221 atatcattcc tggtgaccgt gctgcagatt gtgatgctaa tatggaacct attggagtgt
     2281 gggtgagaaa aaatggtgag tgggcaatta ttcatcgttg tacacgctgt ggacatctta
     2341 gttctaatcg tattgcagct gatgataatt cagtgaaatt aatgtcaatt gcattaaaac
     2401 cactggcaca gccccctttc ccactagaaa gaatcattga aaagttggaa aaggaggaaa
     2461 cagaatgaat ggtatgaaac aaatcaattt acaagactat ggtctaaata ggaattttat
     2521 tactcaaagt atggaatatc acgatttata ccttgctcga attatctcgc aggaaaagaa
     2581 cttttatcaa gctgtatgtg taaatggtgt actgtttgca gaggtttcag gaaaactgcg
     2641 ttttgaggct actatacaat ctgattttcc cacagttggt gatttcgtta tgttggatcg
     2701 ttgcaataat aaaaatggta atgctattat tcatcatgtg cttcatagaa aaagcttgtt
     2761 tgtgcgtaag gctgctggaa aggtaaatga ggaacaggtg gttgcagcta atattgacat
     2821 tgtttttttg tgcatgtcac tgaataagga ttttaacatt catagattgg agaggtatct
     2881 gtctttaaca tgggaaagtg gagctatccc agtagtagta cttactaaat cagacttatg
     2941 tgacaatgtg catcgtaaat tacaagtagt taattctata gccttgggtg tagatgtagt
     3001 agttacctca tctatagagc cagatggtta taaacagatt tctaagtata tcaaatcagg
     3061 ctgtactgtt gcattacttg gctcttcagg agttggaaaa tcttcattaa ttaattgtct
     3121 gctaggagga gaatttttaa atacaaagga aattcgcata gatgatgata aaggaagaca
     3181 tgccacgact agacgagagc tatttttaat ttcaggtggt ggtattgtta ttgacacacc
     3241 aggtatgaga gagttgggaa tgtgggatgt ttcagaaggt ttagataaaa gttttgcaga
     3301 tatagaagct tatttcggaa aatgtcattt tcgtaattgt actcacacct cagaaccagg
     3361 ctgtgctatc tgggaagcta ttgctcatca agaactttcg cctaaaagat gggaatccta
     3421 tcgtaggctt aaaactgaag cagtatttgc agacaataaa agcgaatact tagcaaaaaa
     3481 agaaaagaaa tttaaaaata tctcaaaaat aaataagacg attagaagat aagtgtagtt
     3541 tttttgttca ttctatgagt ctatatacta ttaatgtatt aaatttgcca ttaatttgtt
     3601 actttaatgt gattttgatg gcaaatttgg tttgtggtat tatttaaagc aatttattaa
     3661 tttttttaat aaagtttgat tttttatatg atgatatata tatgtgataa tgtatacata
     3721 tttgttacga tagtctcctt tataaagagg tcttatgtaa gataagaaaa ggcatattta
     3781 tactaataca taatagagta tatgcttatg tatgatagag atgtttctta agagcaaaac
     3841 tgcaatattg ctaatttata aagtaaaaat atatgaatta taaaatacaa ataagatatt
     3901 tataaaaatt attgttttaa tttataataa aaattttttg ttgaaaacaa tgctcgaacc
     3961 tataaaacta ttaactaata tttgtaaatg tatacttttg atgataaaat ataattaagt
     4021 taagttggtt tttataaaca taaatttatt ttagagaggt gaaataaatg aaaatatcta
     4081 gtcaatatag aagtcaatat tcatttagac atgaaagtaa tataaataat acaagaataa
     4141 atgaaagtat ggttaagaaa aatgaaactg taggaaaaga cacttattta tctaatatta
     4201 tgaaacaaaa gcaagaactt aatgatagaa ttagagattt aaaatataga caagaggttt
     4261 atactaaaaa aataaatgaa gcaattaaga acttatataa atcagaaata agagaaacaa
     4321 ctaataactt ttctaatata gaaataggta ttaaaaatag cattatagaa gataaaaata
     4381 aaagtacaat gttagatgaa aattcaactt atctaaatac aaatgatgaa aaagaatctt
     4441 taatcactaa agagtctaat gaaaaaattg aagaagaaat attaaatgat gaaaaattag
     4501 aagagttaga acaaaaaaag gattataaag aggattctaa taaaaaagag aaagtatctg
     4561 aggacttatc tttagtaggt aaaactcgtg aagagcttga aaatatgctt aaaaatttta
     4621 taaatttaac acaagaagaa ataatgaaac ttgagtcgag aatagaaaag ttagataaag
     4681 atgctgaaga atacaaacaa aattcaaaga ctaatatatt tgataaaaca gatgaacaaa
     4741 aaaaacatat aaatgtactg atttaaacaa tatgttttaa aaaaataagt ttaatttact
     4801 ctatggagga aatccaaatg gagttgagct tcgagcagat ggtaaatatt attatataaa
     4861 taagggtatg gaagggctag gattttatgc agcagcattg tcaggtagct catatcagag
     4921 aataggcttt ggaaaacttg accctataga agtaatagcc gatggagatt ggatttctta
     4981 caaacaagct caagatacac ttacagtaat caggaatttt ttaaatagtt ttgactggag
     5041 aaatgcatct gagatggaaa gagctaatag agctgcaaaa ttggtaaccg aagcaaaata
     5101 tgtagactct aaatattgta acatagtcta tggtaatctg gttgataaac gtggtgtatg
     5161 tggaagtttt gcaagtagct ttcatttatt aacaaggtta atgggaatgg attcactatc
     5221 tatattaaat ccatcattaa atcatgcttg gaactatata caaattgatg ggaaatggta
     5281 taggtctgat gggtctgaaa tatcagcttt tggtggagca ttagactttg attaccgtaa
     5341 attaaaggat gcaacaagag agatgactac ttactatgat gctaaggcat tgtctatatt
     5401 aggatttaat caataaaaat aggggaggct ttataaagag ccttttttta ttgatttgaa
     5461 atttaataag gaattttata tgaaaattta atcaataaaa gcaaaaaagg ctctttataa
     5521 agcctttcta agtgtttttt taagtaaaat tttttatatc aacatcagtt ttaatcaaaa
     5581 aatataacat tttggactta cttttaaaaa taaaattatt aaaatataat attaatcatg
     5641 ttaataatgt ttttagttta ttatattcta taatagtggt catgttgact attgtagttt
     5701 tattaagaat agttggttta tataaatata tgcaaagata gcttgttgtt tactttactt
     5761 attagtatat aggacaagat actctaataa atttgcctat tttaatatct tttgtactaa
     5821 aaccaactaa agtttccttt attatttcgt tttgaataat acaaagcata atccgattgt
     5881 ttgtataaag actggaaagt atcagattta ttttctaatt tagatatacc tatagaacaa
     5941 gaattactct ctagacataa ttctaaaact ttctttgctt tattttcaac attctctttt
     6001 gattgattac tatattcaaa aacagcaaat tcatctccac caagtcttcc aataatagca
     6061 tcatttacga atactttagt caaaatattt gaaaactgta ttagtatttc atctcctcga
     6121 acatgaccaa agatatcatt tacctgttta aaattatcta aatcaaagat gtaaaagtat
     6181 ccatatgaaa cagttttaag catttctttt atgtgtactt caaaaaaatg tctattgtat
     6241 agtccagtca taaggtcttt ttctgatttt agtttaaatt catttgccat tgctagtgct
     6301 tcttgtgctt tttcagttat tgttttaggg taatactcat ctttcatttg gtcaaagttt
     6361 ggaatagaat ggtgaacatg gataagttta atcatatcat ctgtaactct gtataccata
     6421 gtaaatcgag tatccatttc tacatagata tctttttcta ggtaatcttt ttctattacc
     6481 caaagagtac catatactat aaaagcttcg tctgtaactt tggtacatga ataccactca
     6541 tctataatct caaagtctat ttgctctgat tctgtttggt ctttttcaag acctataata
     6601 aattcttcta atgtttcgta aaattcatgt tttcctgttc caattaatgc aaggttgtta
     6661 tctaataagg aaataataaa tttaaggttt tcttcttctg gatttaaaat ataaatatgc
     6721 caaatttttt ttgataaatc tcttaattgt ttttgtattg tattattttc catagattac
     6781 ccccttaaga tttacagttt atattttact gtattataag ttgaactctt attacaatat
     6841 tattataata tagaactaaa gaaattacat cctgtatagg taaaagtata tcttttacaa
     6901 taaaaatatt acagtaaaat aaccagtttt aatatacgta ttatattttt aatttataaa
     6961 cgaatattat aaatgaaatt taaattcaac cctgaagaaa taagctaaat gtattagtat
     7021 ataaatccat acttgaacat aaataacctt tatgatatga tatcaataat aatattatga
     7081 gaggagtgcc ttgatgtcgg agttatataa tggtaaccaa caattaaata gttggagaca
     7141 catatattct gtataatcag aaattttaag tgtattttta atacatttaa tttttgtgtc
     7201 ttattctgcc tttatagata actgacatat gacaacaaat agtatttaaa atatatatta
     7261 ctttgttaaa ttatggtgta gtaggcttta atctgctaca cttttattaa ttttgtatag
     7321 tattattttg ataactattt tgaaatagag agcattaatt tttctagtcc acaggagttt
     7381 tctgtttttg tgctgttaaa tataagcaca gaaaaaggca gatatatacc tgtggacttt
     7441 ttttatataa attatgaaat atggaggtag ttaaaatgag tataattgaa cttaatgaac
     7501 tgactaaatc ttatagaaga tttgagaaaa aggcaggatt tatggggagt gtaaagtctt
     7561 tatttaatag gaaatataca gaaaaaacag ctatatcgtc atttgattta aagatagaag
     7621 aaggagaatt tgttggatta ataggtcaaa atggagctgg caaaactact cttattaaga
     7681 tgttaactgg tattattgca ccgtcttctg gagaaatatc tgttatgggt tattacccta
     7741 acaaattaga aaataatttt aaaaaacaat atgctgttgt tatgggccaa aaaagtcaac
     7801 tattctttga attgacacca gcagatacgt ttcttttatt taaagaatta tatgatatat
     7861 ctaatgaaga atataaaaaa aacttggatt actttatcga attatttgag gtatctgact
     7921 atttaaatgt tcaagttagg acgctttctt taggagaaag aatgaaaatg gaattaatag
     7981 tagcccttct acataatcca aaaatattat ttttggatga accaactata ggcttagatg
     8041 ctatagccca aaaacaaatt aggtatttcc taaaagaagt aaatgaaaca aaaggaacta
     8101 ctattatact tacttctcat tacatggaag atattaagag tctctgtagt agaagtattg
     8161 ttgtagaaaa tagccgtaaa atatatgatg gttctacaga gaaactattt aatcattttc
     8221 aaaaatataa aaaaataaca gttagttttt ccaatgaaac taacattgta tttccaaaag
     8281 attgtaaagt tgttgaacaa acaaatcata agacatcagt aattgtgcta aaagaaagag
     8341 gaaatgaaac gattaaatgg atttttgata aatatgatgt taaagatgta atgattgaag
     8401 aagaagatat aggtagtatt gtagagagaa tatataagaa aggaagtgat gagtattatg
     8461 agcaactata atgaagcaag ttcaaattat tcaatgtttt ataaatttaa acaaagtatt
     8521 agaaggtatt atgaagtagc caaaattaac tttaaagtgc aacttatctg gagatttgat
     8581 gtggtggtta atatggtgtt tacgattgta aaaattttat ttgcttatat tttatggaaa
     8641 gcaatttttg gtgaaaataa aatagtctct ggttttacat ttaactctat gttgtcttac
     8701 tatataataa gctcattttt agcaggtatt gatatgtcaa agagtaccag tgaagagata
     8761 tgtaatcgtg taaaattggg cacattttca aagtacatga ttattccaat taatgttcaa
     8821 aattatttta ttgctaaaaa ttttggaatg acatgttttt attttgtatt taatatgatt
     8881 tctgcatttg tatggatttt tatatttaaa atagattttg cctttacatc aaatccagtt
     8941 caaatattaa tttcaatttt gatgattttt ctagggttta tttttatgat gaaactccac
     9001 ttctttattg ggattttgtc atttaaattt cttgagatta gtttttttag aatgataatt
     9061 gaaaatattg ttgaatttat tacaggaaca atgataccac ttgtactctt accagaaact
     9121 atagtaacag ttatgaaggt atttccattt tattatacaa catatttgcc atctatgtta
     9181 ctaataggta gaaacagcaa tgaggcaata caaggattaa ttattctagc attatgggtt
     9241 ttattttttt ctgtagtaaa taaagttatg tataaaagaa tgagaacatt atttgatggg
     9301 gtgggtatat gatgaaaaaa aatttgaaaa taatatttat gttattaaaa atgaaattat
     9361 caaagatgat ggtatttaga tttgattttt ttggagcatt ttttgtagat agttcattat
     9421 ttatattaca acttctaatg tttaatagta tatattctca tgtagactca atagggggat
     9481 ggcaacaagg agagatgtta atatttatag gaactttctc attaataaat gcgataaata
     9541 tgacgatttt cttttttggt atttatgata ttccacgaaa aatccaagaa ggagaattag
     9601 actactatat tacaaaacca ttagacccac tatttaggtt aacatttgaa aatattaata
     9661 ttggttcatc accattaatt ctagcaagta tacttattgt tttatatgga atatctaagt
     9721 taaactcaga gataacttta gttatgataa taatatatat tttcatggtg ttattgatga
     9781 cattactttt ttatgatgta tgtatcatac ttagaacagt ttcatttttt gttatatcat
     9841 cttctgcgat aatgagatta gaagataatt tattgccaat gaatatgaaa attcctggtg
     9901 taatatatga aggtgttttt aaagtattat tctatttaat cttaccatat ggaattatgt
     9961 cgacaattcc gactcaaatt ttggctggga caataacaat taatggatta atatactcaa
    10021 ttttaattgt atttttgttt acattgttta tgtggagatt ttggaagtta ggtatgagac
    10081 attataaaag tgcaagtagt taataaatta gtaaaaatag acaatgtata actatatata
    10141 aatacgtttc atatgacact aaactatgtg aacaagtatt tgcgctaaag gcactgaaag
    10201 ctataaaatg attaaagtaa ggatttaaaa gtttctttag tagaaatata attgtgtaaa
    10261 aaagcaagat taattaaaac tatatccaaa taattagata taattcttat gcagaaagga
    10321 ggtctgtaaa tgtctaattg tttggaatat attttaaaaa taattaatgt acagaaaaat
    10381 aaacaaaatg agtgatgtat attattcaga aaatcatgaa caagataatc tttatgagca
    10441 agataattta ctggcacaaa gatttaattt atttactcta ttgaagtttg catttcctac
    10501 tatttttatg atgattttta tggggttgta tacaatcgta gatactatat ttgtatcaag
    10561 atttattaat acaaatgctc tatcagcaat taatattgta tgtccaatta ttaatcttat
    10621 agttggaatt ggaactatgc ttgccactgg aggaagtgct attgtagcac gtaagatggg
    10681 aaatggtgat attgagggag caaataaagc tttttcttta atcgtattat caggagttgt
    10741 aattggggta ataattactg gtataggtat tgtatttatg gattatatca ttatatggtt
    10801 aggagcaagt gaagttttaa ttaaatattg tcgttcatat ctttttatta tcttggtttt
    10861 tgcttctgca aatatactac aagtattatt tcaatgttta tttgtaacag ctggaaagcc
    10921 tcaatttgga ttatggctag tgttaggtgc tgggtgtgct aatataattc ttgactatgt
    10981 gttcattgtt ctgttacaaa tgggtattag tggagcagca cttgcaactg gagtaggata
    11041 cctaattcct accgtttcag gtatggtttt cttttcaaaa aataaagagt tacttaagtt
    11101 ttgtataccc agactagata aaagaatatt atttgaagct tgttttaatg gttcatctga
    11161 aatggtaggt caactatcaa cagcagttac tactttttta tttaatgcta tgatgatgaa
    11221 attaattggt gaggatggag ttgcagctat tacaataatt atttattctc aatttctttt
    11281 aacttcgctt tatataggtt tctcaatggg tgttgcacca ataataagtt ataactatgg
    11341 aagtgaaaat aatacacagt taaatcatgt ttttaagatt tgtatattat ttatttcagt
    11401 ggcatcttta ttggtattta tcttttctat atcatgttca ccaatcattg tagagatttt
    11461 ttctcaaaga ggaagccgag tttatgaaat tgcaaggtct ggattttata tattttcttt
    11521 tagttttatt ttttgtgggt ataatatttt tacttcgtct atgtttacag cattgtcaaa
    11581 tggcaaggtc tctgcaatat tatcttttct acgtacattt ggatttataa ctataggttt
    11641 attgatactg ccaaatatat taaatataaa tgggatatgg ttagcagtac caagtgctga
    11701 gattttaact ttatttttaa ccattttttg tatatgtaag tataagaata gatataaata
    11761 tatgtagata ttaaataaat agtttaggta gatattttat gttttaataa gtaattcaat
    11821 tagtggtaga tatatactat tgaaaattaa agttataatt gatataaaga attaataaaa
    11881 ataacatata aaagtttaaa agggagttga acttaaagtt tgattagaaa aagtcaattt
    11941 taagacaact ccttttattt tactattgta ttaatctgta tctgtatctg taagtgtatt
    12001 agtctaatat tataaacttt ggatgaaatt aattttctat aggttttaat gtatcataca
    12061 ctttatttta aagaaactta tcttaatttt catagtgatt gaatttgtct attagagatg
    12121 ttactattag taatgtaaaa gcaagagtag tgaatataaa aaaacaagat attgatactg
    12181 caagattaga atataataaa tttttatgga gagatttcaa tatggataca tattttaata
    12241 aaaaaatatt acttgttgat gatgaaaaag atatagtaga tttaattgaa gaagtactca
    12301 taaatgatgg ttttaaaaac attattaaag cttataatgg tttagatgct atttcgctgt
    12361 gtaagatagc atgtccagat gttgtcattc ttgatataat gttaccagat atagatggta
    12421 tagaagtgtg taaaaaaatt cgagagtttt catactgttc tattcttttt ttatcatcaa
    12481 aaaatgatga tattgacaaa atactaggtc ttagtagtgg gggagatgac tatataacaa
    12541 agccattcag tccaagagaa attgcttttc gagttaaagc gcaattacgt agacaacagt
    12601 atcaaagcat tgtaccatca gatagtgcag ttattaaaat aggagatatt actattgata
    12661 tagaaggaaa ccgtgtatat aaagatagaa atgagattga gttaactgga agggagtatc
    12721 atcttttaag ctacatggct caaaatgtaa ataaaattat aggaaaagaa agactctatg
    12781 aacaagtatg gggagtatat agtagtattt gtgacaatac aattatggtg catatacgac
    12841 atataagaga aaaaattgaa gataacccct caaatcctaa aatacttata acggttaaag
    12901 gactaggata taaacttgtg aatagaatcg attagaatag attttaatat taatgaagat
    12961 gtctcaagtt gaatttttag ttaatgaggc attcttttat gtgtgaagtc aaatgtattt
    13021 tcgttatctc aacaatgaag aagaggtcta tctctatttt gagatagcct cattaagttt
    13081 atatcctggt aatgtacttt atgttctaat aaaatttttt tagtttattt gcagtgaaac
    13141 attaactaga acattaaaat ttgcattgaa atatcaatta aataatattg tatattgtgt
    13201 ataaacttat actaattgtt tttctatatt aaacacttta tcaatccaac ctttataaaa
    13261 tttttcttca tgggaaacta ggattatact tcctctaaac tgtttcaaag cattttgtaa
    13321 agcatccttt gtctctgcat caaagtgatt ggttggttca tccagaataa gaaaattaca
    13381 aggagataac atcatacaac ataatttaac cttagattgt tcaccaccac ttaaagtaga
    13441 tacactcctt gaaacatgct cttccttcac accacatctg gctagatgac gtctaatctc
    13501 ttttgtattt aattttggat atttatctgc tactatttga agtggggttt tatcaggatt
    13561 ttcccatttt aaatcttgct cataataacc aattttaact tgttctgaaa actggaaatc
    13621 tccagatata cgaggaatat ctttgactaa tgtctttaac aaggtagatt taccgattcc
    13681 attaaaacca gttataacta ttttttgacc tccatcaaca gaaaaattta attttggaag
    13741 taaagaatag tagtatccaa cttcaagatt tgttatagta agagcatttt gagccgatat
    13801 ttcaatttct gaaaattgaa tatttggttt tcctgtaaaa ctaggaggag ctattctttc
    13861 tattttatcc aattgttttt gtcttcctct tgcaattttt gaatttactc cagctttatt
    13921 tttacgtata aatgtctctt ctttttctat tttcttttgt tgtgcttgat accttctaat
    13981 ataatcttct cttaaatgtg atttttgttt aaggaactct gaatattttc catgatattt
    14041 tttaatcata ccaaattcta tatcaagaat acctgtagaa attttttcta aaaaatcaaa
    14101 atcgtgggaa actacaataa atgccccatt aaatgtattt aaataatttg agagccattc
    14161 aacatgttct ttatctaaaa agtttgttgg ttcatctaat aatagaacat catggttaga
    14221 aagtagcaat tttgctaaaa ttacctttgc cctttgccca ccactaagct cttcaacaac
    14281 acgattcatt cctattgaat ctaatccaag accatttgct attttattta tttcattatc
    14341 tatagaatag aaattacttg ctattagttg ttcctgataa tctgatgctt tgattagata
    14401 ttgctcattt tcagatatag cacttttttg atagagtaaa ttcatttcct tttctatttt
    14461 ataaagttct tcaaatgcag tgtgtaaata gagtgatatt gttgtatctc tattgatttc
    14521 tgcatattgg tctaaatggc ctatattgat attaggttgc cacttgattt ctcctgaatc
    14581 tggaacaact tctcccaata aaattttaat taatgtactt ttacctgtac cattttggcc
    14641 aactacacct atatgttcac ctttgaataa atcaaagtta gctttctcat acagtgattt
    14701 atccataaaa cattgtgata aattagttac ttttaataaa ctcataattc aaactccttt
    14761 tcttttatta aaaaatttat ggaaataaaa aaagcagagg aatggcattg taaaatgccg
    14821 acctctgctt ttggaaaaac aaaaggtcat agacaagcat tgttgtctat gaccagaaaa
    14881 tctatactat ctatcggcaa ataggtaatt ttaaataagc atagccaaaa aactatacta
    14941 aacctatatt tgccgatgaa gatagaaagc tttaagcttt cagattaacc tctacacaac
    15001 gcttcatcgt atacatttgt gtagagttaa gttttctaac aagtttaact aattctatat
    15061 aaatcataat tattcacatc ctaattttag tattaattct agtttatcta tatttaaatt
    15121 gtacttttgt actttgtgga ttatcttatt tatttaaaat atgttttatt aattatagct
    15181 taagttatgt tatcataata cttggttata gtcaataaga aagttatatt tgctatatat
    15241 tatattaatt tagtaaatct atcattacaa agttatttga agactcgcta ccaataaaat
    15301 tttatagtct atgggaaaat taagattatg agttttatac ttttgatata gggatactat
    15361 tttcataaat attacaacat gtagaagtat atgtctagta ttgttgaatt ttcaatgata
    15421 tattaaggtt ctaatatatt atacaaaata aaaacattaa ttcaacttaa ataattttaa
    15481 gtattatatg tgttttgatt aaagaaaact aaaattttaa ggaaaaataa catatttgta
    15541 ttaaatttta ttgaaatgat taaatttatt tacttaatgt aaattattag ctaaaattgt
    15601 attaaataat taaggaggtg agtagctctg gatgaaatag atttaaaaat aattaattct
    15661 ctaaaaaaga atagtagggc ttcaacctct gaaataagca gacaaattaa tttatcagta
    15721 ccagcagtct cagagcgtat aaggaaaatt gagcagtcaa atttaattca aaagtacact
    15781 attcaaatta atcgggaaaa aacaaaatat aagcttttgg cttttatatt tatcaatact
    15841 gatttatcta aaagtatgaa agaatttaag aaaactatat tggaatatga tactgtatta
    15901 gaatgtcatc atatttcagg agagtatgat tacttaataa aagtattagc agaagataca
    15961 agtaatcttg aagagtttat atcagtgact ttgaaggaaa ttggggggat tcaaaaagct
    16021 aatacaacca ttgttttatc tacaattaaa gaagaattta atacaataaa agatattttt
    16081 taaataattt actagtaaag tgttaattat attttaggag gaaaatagaa tatgaaagtt
    16141 ttagtttttt tagcaaaagg ttttgaaact atggaattta gtgtatttgt agatgttatg
    16201 ggatgggctc gtaacgatta tgggcatgat attgatgtag tgacttgtgg atttaaaaaa
    16261 caagttatga gtacatttaa tatacaagta ttagttgata agacgataga agaagtatgt
    16321 gttgatgatt atgatgccct agctattcct ggaggatttg aagaatttgg attttatgat
    16381 gaagcatatg attcatcttt tttaaatcta ataagggagt ttaattcaaa agaaaaaata
    16441 atcgcttcta tttgtgtggc agcattgcca gttggaaaaa gtggtgtttt aaaaaatcgt
    16501 aaagctacta catatcattt gaaaaatggt aaaagacaaa gacagttaag tgagtttgat
    16561 gtaaatgtag ttaatgaacc aattgttgta gataaaaata ttataacctc atattgtcca
    16621 gaaacagctc cacatgtagc ttttaagctt ttagaaatgt tgacatctag agaacaaatg
    16681 gatgaagtta aattagcaat gggatttaaa ctgtaatgct ctatagactt tctaagtaat
    16741 cagaacccca gtcacacatg gaatctaaaa ttggaataat actctttcca aaatcagata
    16801 gcgagtattc tacttttgga ggaacaacag gataaaccac tcttataatt aaaccatctg
    16861 attctaattc acgtaattgt tgagttaaca tcttaggcgt tgctttagga attaattttt
    16921 ttaattcatt aaacctcaat atagtgtctt taagatgcca aagtataagt gtcttatatt
    16981 taccaccaat caatgcaagt gttgcttcta ttgggcaact gtaattatct aaacattttt
    17041 tcatttttga gctcctttat ttattttaaa gtatcttttt agatactata ttacaaaaaa
    17101 gtgcattatt gtaatatagt accatagtga taaaattata gcataggtta aataaatcat
    17161 caacttagcc taataaaaca atagggggta aataaatgaa tttcgttgaa cttacaaaaa
    17221 aaagatattc ttgccgtaat tatcaagata gaaaggttga aaaagaaaaa cttgagaagg
    17281 ttttagatgt tgcaagaata gctccaactg gtggtaatcg tcaacctcaa agattgattg
    17341 ttattcagga aaaagaaggt ataaataaac tttctaaggc tgccaatata tatgatgctc
    17401 cattagcaat tcttgtatgt ggagataaag acaaagtttg gacaagacct tttgatggga
    17461 gacaacttac agatattgac acaagtatag ttacagacca tatgatgtta caggcaactg
    17521 aacttggatt ggcaagtgtt tgggtgtgct attttaatcc agatataatt agagaagagt
    17581 ttagtttgcc agataatcta gaaccaataa atatattatt gatgggatat gaatctaaga
    17641 taccagagag cccagagaga catgaaaaaa ctcgtgttcc tttaagtgaa attgtttctt
    17701 atgaaactct ttaattataa aattaagtta ttcataggat aaatgtaata aagtgtttat
    17761 tataaacaaa attcaaaata aataatatct acatataatt aattttagtg aagtatatct
    17821 agtttaacta gaatgtatac ttcactaatt tatttattca aaatatttat gaggatttta
    17881 atatattaac taaaatatat caattgaatt aacttttaaa tctggaaaag cacttgaaat
    17941 atagtgtttt atgtcattta aatggtcttg aatttgcttt tctgatattt tatcaggtcc
    18001 atatacaaaa tacataatat ttttagtatc ttttgttata gctgttcgat tatcagggcc
    18061 attaagaata ctagataata taccaatatt atctgataaa aataaatcat cttttgttaa
    18121 aatatgctct ttaccaccta taccataaaa atgttcactt ccatttgcta attctatttt
    18181 caaaggtaat tccattttat caaggtcatg acctgctgtt agaagaagat ttttcaattc
    18241 tgctaaaaac attgactcaa ttgttacacc tacatctggg ataccttttg attttaatag
    18301 aatagattct aattgaagca atacagggta agttttcttg aattttttat aatactttat
    18361 ataacttgaa acaggctctg ttttaatttt ttctttacga ttgtattctg ggtgttgttc
    18421 tataatattt ttaattgtag agtttttaag aactaaaaaa ttttctttat taggttttga
    18481 atgtgtaata tttataacca taactccaaa tttaatattg ggataaattt ctttaacttt
    18541 atgtgatatt tctaacataa tatagactcc tttttatttt atattttatg atataatctt
    18601 agcaagttaa aagtggattt tgtagttcca tttttatagt aattttatag aaccactttt
    18661 ggaggtatta gaatgtggat tacgattaat agaaatagta atatatcatt atcaagacaa
    18721 atttattgtg agataaaaga tatgattctg gatggaagct taaagtttga gcaaaaactg
    18781 ccatcatcta ggactttatc aaaagaacta tctgtatctc gtaatacagt tttagatgca
    18841 tataatcaat taattgcaga aggatatttg gaaacaagga atggatatgg aacaattgta
    18901 gctaaaggaa tatcagggca taaagttgat tttaagttaa atgatacttt tagagatgaa
    18961 aaagatttac atactaataa acaaaaaaat tatatagatt ttcgttcagg agttccagaa
    19021 ttaaaactat ttccacgcaa agaactttca aagctatata aacaaatact gtatgattta
    19081 tcagattcgg atttcagata tggaagtaca gctggtgtat gggttttaag agtggaaata
    19141 tctaagtatt tatttcgtac tagaggaatt agatgtaatt ccaaaaacat tatgattgtt
    19201 tctggttcaa cacaaggttt atctttaatt tcacagcttt tatacaaaag taataaaaag
    19261 gttgctgtag aagaccctat tcataaaggc ttattaaagg ttatttcatc tattggatat
    19321 tcagttgttg gaattaaagt agatgataag ggaatgaata ccaacttaat aaaatcaata
    19381 aaggggattt cttttgttta tactacacct tctcatcaat atccattagg agggatttta
    19441 cctattcaaa gaagactaga gcttattaaa tgtgcaacag agaatgatta ctatataatt
    19501 gaagatgatt atgatagtga atttagatat gagggtcaac caattagttc tctttatgaa
    19561 cttagcccaa ataaagtaat ttacataggt tcttttagta aaatattatc tccagctatt
    19621 cgtgttggat ttatgttatt accagacaat attttagaaa gctataaaac cttaaaaatg
    19681 tactctgatg ttcatacaga aacaatattg caatatgtat tagctgaatt tattggaaat
    19741 ggaggtttag agaagtacat atttagaatg aagaagttgt acaatagaaa acgtaaatat
    19801 ctcataaaag ttttaaacac ggaatttaaa aatgaattta aaataaaagg tgaagcagca
    19861 gggcttcata tagttgctca tttctataat gttaaattta cggaaagttt aattcataaa
    19921 atttttgaag caggtataaa agtttatcct atacaacaat atgcaattga gaatatatta
    19981 gaatatgacc atgaaattat actaggttat gctcatctta gttttgatga aattgaaaaa
    20041 ggtgtaaaaa tactcagcaa aataatacat atgaatcaag gtacatagat aaaacatgta
    20101 gcaatctaca tataatagat gtaattaagt ttaaacttta aaagaaatat atattaatat
    20161 ttttgtataa aaat
//
LOCUS       DAELXX010000072        19953 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.072, whole genome shotgun sequence.
ACCESSION   DAELXX010000072 DAELXX010000000
VERSION     DAELXX010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19953
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.072"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(220..924)
                     /locus_tag="KPH29_002453"
     CDS             complement(220..924)
                     /locus_tag="KPH29_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HBG1468039.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVIICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEVSVD"
     gene            complement(1278..2294)
                     /locus_tag="KPH29_002454"
     CDS             complement(1278..2294)
                     /locus_tag="KPH29_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBG1468040.1"
                     /translation="MGYYNDNDNNKIRRKKISNNKDSNKKSKHSYTSANKKNKSPNTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     gene            complement(2422..2928)
                     /locus_tag="KPH29_002455"
     CDS             complement(2422..2928)
                     /locus_tag="KPH29_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HBG1468041.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            complement(3087..3755)
                     /locus_tag="KPH29_002456"
     CDS             complement(3087..3755)
                     /locus_tag="KPH29_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468042.1"
                     /translation="MENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKIKSR
                     NGFRYDRDFIEARREEIHIKDNNNNKNKSNNKKLKEEKVFKYDNGDLYKGEFVDGKKN
                     GFGIYIFSSKEKYEGLWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDI
                     YKGYFLDNKKNGKGVLYKNDGNKQAGIWENDEQCKSLDFKDLNNNKINNVYQNRLNNN
                     KLKV"
     gene            complement(4168..4674)
                     /locus_tag="KPH29_002457"
     CDS             complement(4168..4674)
                     /locus_tag="KPH29_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HBG1468043.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTVAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
     gene            complement(5045..6073)
                     /locus_tag="KPH29_002458"
     CDS             complement(5045..6073)
                     /locus_tag="KPH29_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBG1468044.1"
                     /translation="MKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVMYDEKTKNIDKF
                     KSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYTFGN
                     LDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLEFVN
                     LEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCFNKF
                     KESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDINKKMDTIDKLKDI
                     EEGYFYLLEDVYLDMKDVLEDLMLYRMNEDEYKYEIKNIIKMFDEDLDNEKIYINKCN
                     NLNVVNKI"
     gene            complement(6121..6981)
                     /locus_tag="KPH29_002459"
     CDS             complement(6121..6981)
                     /locus_tag="KPH29_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBG1468045.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKSI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            7260..7469
                     /locus_tag="KPH29_002460"
     CDS             7260..7469
                     /locus_tag="KPH29_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBG1468046.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            7469..7735
                     /locus_tag="KPH29_002461"
     CDS             7469..7735
                     /locus_tag="KPH29_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBG1468047.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            7764..8336
                     /locus_tag="KPH29_002462"
     CDS             7764..8336
                     /locus_tag="KPH29_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG1468048.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(8570..9760)
                     /locus_tag="KPH29_002463"
     CDS             complement(8570..9760)
                     /locus_tag="KPH29_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG1468049.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENVENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKVSYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSAGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(10020..10451)
                     /gene="dut"
                     /locus_tag="KPH29_002464"
     CDS             complement(10020..10451)
                     /gene="dut"
                     /locus_tag="KPH29_002464"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBG1468050.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            10564..12792
                     /locus_tag="KPH29_002465"
     CDS             10564..12792
                     /locus_tag="KPH29_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HBG1468051.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEYEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEF
                     DN"
     gene            complement(12903..13388)
                     /locus_tag="KPH29_002466"
     CDS             complement(12903..13388)
                     /locus_tag="KPH29_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG1468052.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
     gene            13640..14863
                     /locus_tag="KPH29_002467"
     CDS             13640..14863
                     /locus_tag="KPH29_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBG1468053.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTKESEPEKPIAVSVQKAVGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPNILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNANNKFKAGMSAQVNLSIEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
     gene            14867..18001
                     /locus_tag="KPH29_002468"
     CDS             14867..18001
                     /locus_tag="KPH29_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HBG1468054.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGEKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            18035..19333
                     /locus_tag="KPH29_002469"
     CDS             18035..19333
                     /locus_tag="KPH29_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HBG1468055.1"
                     /translation="MSKIVAIGCGVGIITANLIPVYADNNTSSINNVDNSILSDSNKR
                     TSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFPYDQI
                     GLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMKLKKD
                     LGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEYRFES
                     FRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVAPTKP
                     EQGNLTDDEYKVLMDKYEKDYKRYEKEVEAYNLERQTYAAGLTTYANYLQQKFNTENG
                     LVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGLITLT
                     DYKKQVVSNQDAKNSYDTLIVNYNSLKNGIEKPWILNTGK"
     gene            complement(19543..>19953)
                     /locus_tag="KPH29_002470"
     CDS             complement(19543..>19953)
                     /locus_tag="KPH29_002470"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HBG1468056.1"
                     /translation="NVAKDLFNGEIVAYEISTKMDTKLVNKTVNQLINMNLAKDCILH
                     TDQGSQYTSRSYSKRLKDNGIIQSMSRRGNCWDNAPIESFFSHFKSELIYLIDTTDPK
                     EMISLINDYIYFYNNERIQLKNGMSSIEYRTHSA"
BASE COUNT         7127 a         3003 c         2608 g         7215 t
ORIGIN      
        1 aattaacaat atatttttat tgttaattat gcaaataaaa aagagtataa taaaagcctt
       61 taaaatacat ttttttctta ttttaaaggc tttttatatt ctacattttt attttctata
      121 ttatatcctt tgataatcgt tacatctaaa atagaactta ttaccctaaa tataagtatc
      181 tttataacta gaatagtcca ttacaacagc atctccagat taatccactg atacctcttt
      241 attatcaata tctaaaattg aaattttaca attcccaccc aatttttctt gaactatttt
      301 agctaattca tttaaagcat tttcaaattc tttaaatcta ctttcatcta ctaattcaat
      361 gcataaaaag gcattgttag tatcttcagt aagcattgtt ctcactgctt cacgccaatt
      421 ccaacatcta caaattgctc caccatcatc tttataaatt atttctcctt catatggaaa
      481 tgaattttca tctgtcccta gtgggataaa ctcctcatta ccaattgctc tagttagcct
      541 aatatctcct ataaatttat ctatatcctc ccctccacaa ggtaatgcat attttaatga
      601 gataaaattg tagatatcca ctaaagggtt aatagttcct atattatttc cattatgaac
      661 tctttttaat aatgcttcaa tagaagacct tgctcctttt ttcgttttaa attttttaaa
      721 tgcctctctc catactttaa tcacttggtt gttgctaaat tctgagtcct ccaagtattt
      781 taatgcttct tcttctccct tcaaaattat tttcttatag tcttcatctt ttatagagtt
      841 atctatatca ttacaaataa taactccaat ttttgcacta ggaaatagat tccaaaatgc
      901 atcctcaata acaaattttt tcattagaaa acctcctcaa atttaaaata tgtaaaaata
      961 aaaaagccta tagaaatatt attaaatatc tcccaggctt ttatccttcc gtgtacacaa
     1021 caattgcgcg ttttctcttg gacctgacca ttataaaatg cggaacccta gaaaactaaa
     1081 ttataagttt tttaatttac aaaatacaat taaattttag cataatatag catttaaaac
     1141 aatattctat tatctagttt gtacattaaa acaatttgcc attagattat aattatggtg
     1201 gcaaatgggt ggcaaatcac cttttttatt acatttttgt ttgtttaaac tattgaaaat
     1261 actatattct tacaatttta atcaatttct gatttaaatg gagtgtcatc agcttttgct
     1321 attctatact ccttatcagt tatataacca agctgatgca tcttatacag tacaatcact
     1381 tgtctcttct tagcctctcc atggtcaata taccttgcag gattatttgt cataccagct
     1441 aacatagcac actcagctaa atttaattca ctaacttttt taccaaagta gacttcagct
     1501 ccctttgcag ctccatatgc atttttacct agatacatat tatttaaata tgcctcaaga
     1561 atttcatcct tagtcataat cttattcatc tgcttagcat tatacatatc ccttattttc
     1621 cgcttcatag ttttatcatt actagtaagc aggttttttg aaacttgcat atctatagta
     1681 cttgcacctt gtgttgtatc tgtaaacaca ttgtttaata ctgacctaac tagagaaata
     1741 acatctactc ctttatgctt ataaaatctc tcatcttcta tggaaactat tgcatgtttt
     1801 aaatcatttg gcatttgttt tatacttaca acctcactac ttatatattt agacctaatt
     1861 aactttttag taacttctgg tgaccctttt atagctgaaa ctgtaaatat tgcacctgcc
     1921 actgaaaata taattagtgc aactaacata atagataata atgccctaac tattctaatc
     1981 cttttttgtt tttttctttt tttacttttt aattttttct ctctttgttt atactcgact
     2041 cttcttcttt ctctttgatt gtaatctgta tttaaaggtc ttacattatc tcttgtaaaa
     2101 ctatctctat ctctttttat aagattctgt gaatctactt tttcactcat tcttttactg
     2161 gaacttgtat taggactttt atttttttta tttgccgaag tataagaatg tttagatttt
     2221 ttattactat ctttgttgtt actaattttt ttacgtctta ttttattatt atcattatca
     2281 ttgtaataac ccattttagt ctcccttctt catataaggt acattataca ttgttctaca
     2341 aaataaatca aagaacaaaa ttgtaagctt aagggtcaat tttataaagg atattttcac
     2401 taattttctc tatatttata attaatattc atcttttatt ttcaaaaata gttttgcagt
     2461 tgctaatgta tcactgtcag ctctatgagc atttatattc tctatgttgt aataagcaca
     2521 agctgtttca agtttcttgt tttttccttt ataactttta cattttttta ataattctac
     2581 tgtacatata taattgttta ttggttctaa atctaacatc ctaagatagt aatttaaaaa
     2641 ttttaaatca aatttcgcat tgtgtgcaat tatagttcta tcacctatgt attttctaaa
     2701 tctaggtaaa actaattcta gtggttcttc attttctacc atttcattac ttatatgtgt
     2761 caattctgtt ataaatgaag aaattatccc cttaggtttt atcaaccttg aatagttttt
     2821 tacaatttgt tcatttttta tttctgttat tcctatttct attatttcac aacctttata
     2881 tggatctaat cctgtagttt ctaaatccac aactacatat tctttcatat ctcttctcct
     2941 tattattaaa tggatatatt tatattctat atttatgtaa tagactaatc aatacttaat
     3001 tttatatgat ataggaagat tttacaaatt acaacataga tatatcctat attccatcat
     3061 aaataaagat ttttttatta gaataattaa acttttaatt tattgttatt taatctattt
     3121 tgataaacat tatttatttt attattattt aaatctttaa aatctaaact tttacattgt
     3181 tcatcatttt cccatattcc tgcttgttta tttccatcat ttttatataa tacaccctta
     3241 ccatttttct tattatctaa aaaatatccc ttataaatgt cattattggg atatgttaat
     3301 tttcccaaac cattttttaa tccgtattta aattcacctg tatatattga accatctcta
     3361 taagtatatt ttcctattcc atgcatcttg tcatctttcc ataatccttc atacttttcc
     3421 ttacttgaaa aaatatatat tccaaatcca tttttctttc catcaacaaa ctcaccttta
     3481 tataaatcac cattatcgta tttaaatact ttttcttcct ttaatttctt attgttactc
     3541 ttatttttat tattattgtt atcctttata tgtatttctt ctcttctggc ttcaataaaa
     3601 tctctatcat acctaaatcc gtttcttgat ttaatttttt gattaatctt cttcttatta
     3661 ataaatactc tagctagttg agtaaatata aatacagatg ataagcatat aaatacacat
     3721 agtattgttg taaaataact agttatattt tccataaaaa atcacccttt aaaaaattta
     3781 tttaaaatac ctaatataat atatattttt attaaatgtt atttatactt ttatagtact
     3841 atattgaata aaattaactt tgaaattatt tacattaaaa taaatcaaat acaaatgcca
     3901 taaattaagt gtttctagtt agcataaaaa tacttataaa gtatgctcaa atatttcaaa
     3961 taaatacttt tattcaaata aatctctact tatataagtg cttatataaa taaaaagatg
     4021 aactatagta aatatagttc atctttttta taatagtatt tatttaaaat taatatttat
     4081 ttagttagta gcatctactt agatatcaga taagaaagca cagtcattta cttataataa
     4141 gttcttttca tatatttttt attataatta ttcttttaca agaactttta taatttctga
     4201 aatgtacata gtatgatagt ctttctctgg ataccatttt ttatcatttt ctttagcatc
     4261 aaagttttct ggtggcatat taccatgata tagcttttta catactataa tcatattagc
     4321 ttcttcaaaa gctaccgtat catccacaaa atatggtgta agtcctgctt tttctatttt
     4381 atttatatct ctaccagaaa tagtaccaca aatatttaaa gcctctctat acgtttcatc
     4441 aaagaaggca actgtaaatg tatcattact atctacaaat tcttttgtat atctctgtgg
     4501 tcttatgtaa gttgtaacaa catttttacc ccaatagact ccaacaccac cccagctagc
     4561 agtcattgtg ttaaactttt ctgaatcacc agctgttata agtaaccatt ctttgccaat
     4621 cttagtaaat ggattaaact gtagttcctc aatttttact tctctaaaac ccatgacttc
     4681 atcctaccct tcatttttta attatataaa ttaattgtta aaaatttgaa tatatgtact
     4741 cttataatac tgacttttga ttgtcctaca taattcatgt aaattataat acaacaaatc
     4801 aattactttc gcaataagta gttttcatat tttataagtg taaaatatag ataaaatata
     4861 ttgattataa agtatataat atatttttaa acctaaagca atatacaata aaaaaatgta
     4921 gaaagtaagt atctctctac attttttatt atatattatt tttatattaa tttaatttta
     4981 tcttgattca gttttataaa aaattgttat aattgtcaag ttctactcta ttatttgtaa
     5041 tttcttatat cttgttaaca acatttaagt tattacattt atttatatat attttttcat
     5101 tatctaagtc ttcatcaaac atttttatta tattttttat ctcatacttg tactcatctt
     5161 cattcattct ataaagcatt aaatcttcta atacatcttt catgtcgagg tacacatctt
     5221 ctaataaata aaaatagcct tcctctatat ctttcaattt atctatagta tccatttttt
     5281 tatttatatc tttcataatt tctaaatttt gtatatttac atctatgatt gttaataagt
     5341 acattctcac agtttttatt atttcaacag aaaaatctac aatattataa tttccttctt
     5401 ttttatataa tgactcctta aatttgttaa aacaattatc taaattagaa tttatatcgt
     5461 taaatggtgg tcttattatg tcacgtatta ttaaaagatt ttgtatatct acattatttg
     5521 tgattgcctc atccaaaata aataaattat atgcatcatt taatgggtca tgggcatctc
     5581 cactaaactc taagttaaca aattcaagta aatttttcaa tgatatatag ttcatacatc
     5641 tcataccaca atttatatat ttttctttta tatctacaaa taaatctcta atattattta
     5701 caaaccttgg atggttattt tttttagcac ttctcatatc tgtgttattg aaacatgtca
     5761 aatccaaatt accaaaagta tatattcctt taatatcaga aaatatgcca agccattttt
     5821 taaattttct catgacctct tcataacttg gtgcactctt caattcttca gaagatatat
     5881 gtgttaattc ttgtatatga ggatataact cctcattact tactggtctt attaaagatt
     5941 taaatttatc aatatttttt gttttttcat catacataac tgctccgatt gcaattatat
     6001 cagaattaaa tactgctctt tttgatttag aatttggcat attcatttca aaatctatat
     6061 atactctttt caatataaac accctttcca attgtttata gtgattcttt ttaactattt
     6121 ttattcaaaa ttttctaaga aagaatgttt ctccccatct ttttgccaac caagtactga
     6181 atcaagattt acattttctg ctgccttaaa tatagatttt tctacatttt taaaatttga
     6241 ttctaagctc tttattaagt cttttatctc atatctttta ttacctgttt ttttagcagc
     6301 aaccatacat gcacaattta atggccatat accactattt tgaataaatc ttataatatg
     6361 ttcttctctt atataataaa gtggtcttat aatttttata ccttcaaaat tagtagaatt
     6421 aagtttagga agcattgttt taaaatttcc agcacacaat aagttaagca ttgttgtctc
     6481 tattacatca tcataatgat gacctaatgc aagtttgtta caccctaatt cttctgcctt
     6541 tgaatataat gctcctctcc tcattcttgc acacatatag catggataat cttttgctat
     6601 ttcatctgct atctcaaata ttcttgaatc aaataaatgt attggtatat ttagatactc
     6661 acaattatct ataagtaatt gtcttatatt tgcatggtat ccagggtcca tagcaataaa
     6721 ttcaacatca aaattaactt gtccatgttt ttttaactct tgaaacattt tagccataag
     6781 aatactgtct tttccaccag aaatcgctac agctatctta tccccttctt caaccaattt
     6841 ataatctctt attgacttca taaacttaga ccataaattt tttctatatt tctttattat
     6901 acttttttct atatctctta ctggaagtct ttcctcaaaa ggaactataa tttcacagcc
     6961 ttttcctgat agttcactca tctaatcacc tcgtatattt atgtatattt atgttaataa
     7021 tattttacaa aaaagtctac cttcatccac aaaatactat aatttttact ttaattttac
     7081 taaataattt atagtttaca ataacacata aacttataat aacataaata acctctttct
     7141 tttattatct tttttatttt tctcatttaa tattttatta ttagtaacga tttttaaatg
     7201 attgaatatt atacactaga tagagttacc tatctattta caacaaggag gttttcatct
     7261 tggaaaataa gactagagat atcaaaaaaa attgtggtcc atctttggct agaccataca
     7321 ttaataatca aattttcaca gaaatgtact gcctttctga tgcattaaaa tttggaacta
     7381 tatttccaga actaaactta ctagagtctg aaatgtacaa caaagaatta tatgctaagc
     7441 caaaaaaata tagaggaggt agaagataat gaaagactca tctagagctg aacttatgag
     7501 aaaagttcag gaaacatctt ttgcatgtgt tgatatgaac ttatatttag acaatcaccc
     7561 tgatgataaa aatgctatca acacttataa ttcattatgt aatcaatttg cgcaagctag
     7621 atatgcttac gaaaataaat acggtccttt aactaacttt gggtatgctc caagtagatg
     7681 cccttggcaa tgggctgacc aaccttggcc ttgggacaga gaatttaatt attagacatc
     7741 aagtattgga ggattgaaaa ttcatgtgga tatatcagaa aactatacaa cacccagtta
     7801 atataaaaac ttgtgaccct agaatggcta aatttcttat aactcaattt ggtgggccaa
     7861 atggagaact tgctgcatct ttaagatatt taagccaaag atatacaatg cctactggta
     7921 atatgcgtgc acttttaaca gatattggta cagaagagct agctcacgtt gagcttatat
     7981 gtactatggt ttatcaatta acttctgatg caagcccaga agagttaaaa gctgcaggtc
     8041 ttggttcaaa ctatgctcaa aatggatatg gaatttatcc aacagattca aatggtgttc
     8101 catttgatgt aagacctata gcagttatgt caaatcccgt aaccgactta catgaggata
     8161 tggcagctga acaaaaagca cttgcaactt attatcaact tataaaccta acagatgacg
     8221 ttgatgttat agatgtatta aaattcttag gtcaaagaga aataattcac tatcaaagat
     8281 ttggtgaagc tttaatggat gcttacgagt tagaagaatc tcaaaaaatg ttctaaaatt
     8341 tttatttgta taactattat tatttgataa tttataattt actgttgtgt taaaatttat
     8401 aaaaaatata aatataattt attttctata aacagagaaa ataaataaaa agcaggtatc
     8461 aatcaaatag taacatgtat tatactatac attactatca atattgtacc tgctttcata
     8521 ttataagcaa taaagaaaaa tctatttatt aggttttata ataaaacaat tataaaactc
     8581 ttcttgcatt tacatatcta cttgaatagt agctactatt tatattagat attgttactt
     8641 tttttgatga acctgaagat gcatgtatca tatctccatt tcctatgtat attccaacat
     8701 gacttacaga accattattt gaaccttgtg tatcaaagaa tactaaatct ccacttctaa
     8761 ggtctcctct atttacatat gttccatact tactttggtc tctagatact cttggtatac
     8821 taactcctgc agattttttc ataacgtact gtgtgaatcc agaacagtca aaactatttg
     8881 gaccttctgc tccccataca tatggttttc caagtagtgt ctttgcaaaa cttattacac
     8941 tatcagctcc ttgacttgga gaattagtcg aataagatgg aattgaagta tcaacagaac
     9001 ctttttcagc aaggtattga cttgacacat atccagtttg attgccataa cttacttttg
     9061 tccatcctcc actttcagaa atacttccaa cctcagtacc ataacttaat gtagctattt
     9121 tagaatgact tgtacctggt cctgttctaa cgttcaatga ctttgctgtt acaacctttg
     9181 taccctttac aaggtcattt gaactgtttt caacattttc atttgtagag tcatttacac
     9241 tagttcctaa atatttactt gaaacataac ctactctacc attagaactt atttttgacc
     9301 atccatttga ctcagatatg tatccgactt cagtcccata tcctagtgtt gatattttag
     9361 aactacttgt acttggtcct gttctaaaat ttaatccttt tgctgttaca acttttgtcg
     9421 acttaactga agtatcacta ctttcattgc tacttccaag gctatttatt gctacataat
     9481 ttccatgcac ataacctgtg tttccattat atttaacttt tacccaagaa ccttcttttc
     9541 ctacgtactc aactttatca cctttgtaaa gtttccccat tgattcatgg tttgtacctg
     9601 gtccttttct aaaatttacc gcattccctg ttattgttct gctttctata ctcacactct
     9661 ctacttcaga ctcattagca tatgctaaat tccccatagt aggcattatt atcgcagttg
     9721 ccattatact ggctgctatt ctcttttgat taaaattcat aactttccac tcatctcctc
     9781 ttttctacta tctttatttc acttcaaaat ataaaagaaa taattgacac tattaaaata
     9841 tcaattattt cttttatatt aatattatta caaatttgta actatttgtc tatagtaata
     9901 tttgcttacc ataaattacc atgtataaat tttaatcttc aatagcctgt aatttagtaa
     9961 tatagccatc ttttaccttt tttaaaaatg gctatatttt ttaacttttt tgtaactttt
    10021 taaaatccag ttgagccaaa cccacctttt cctctttcgg agtcacttaa ttcttttact
    10081 tcttctatgt taatatcata gataggtttt actatcattt gagcaatttt catatgcttt
    10141 tcaaccttaa aatcattttt cccatgattt attaatataa ttttaagctc tcctctgtat
    10201 ccttcgtcaa tagttcctgg tgtatttaat acagtaacag aatgctttaa ggcaagccca
    10261 cttctaggtc ttacctgcgc ttctgtcata gtaggaagct ctatacatat cccagttttg
    10321 ataagtttag tctctccagg aggtattaca acctcttcta tagaatacaa atccatacca
    10381 gcatcacctt tgtgagcaaa attaggtatt atagcatcat catttaattt ttttactttt
    10441 aaattgtaca tcttatatct cctttcaact ttctataaat attataactt aaatttcgac
    10501 ctcttgaagt tttttatttt tttgtggtaa tataattatt agtgttttaa aggaggattt
    10561 tttatgaaac ttgtacttgc tgagaaacca tctgtggcaa aaactatagc atcattttta
    10621 ggtgcaaaga ccagacaaga tggttacttt gaaggaaatg attatataat tacttatgca
    10681 gttggacact tagtatcatt atatgatatg aaggattatg ataaagataa atactctggc
    10741 tcttggaaaa tgaacaattt cccatttgtt cctgaggata agtttaaatt taagattgat
    10801 agttctaaaa ctaaacaatt caacactgta aaaaagcttt tgaatagaaa agatgtagaa
    10861 tatgtaataa atgccactga caatgaccga gaaggagaac ttatagcatt tttaatattt
    10921 ttactggcaa aaaataaaaa accagtaaaa agaatcttgg taaatgaatg gacaccagaa
    10981 gatattacaa gaggtataaa aaacctaaaa gatgaatatg aaatgagaaa tcttcaagct
    11041 gctggatata ctaggcttat tactgactgg ttgattggaa taaatttcac ttctgttgct
    11101 acattgaaat atggaaatgg gaaactatta aacataggta gggtaattct tccaacagtt
    11161 aaattagtct atgatagaga tatggaaata ctaaactttg ttcctaaaac ttattatgaa
    11221 atagaaggac attttaaagc tgaagctggt gaatataaag gtaaatatgt taagggtaaa
    11281 gaaagtaaat ttgatacact tgaagatgca aataaaatta tagcttcaat tacatctgaa
    11341 actggtaaaa tactagataa aaaagttact atgtcaaagg aatatgcacc aaaacttttt
    11401 agtttgacat cacttcaagg atatataact tctaagtatt ccaattttac atctgataag
    11461 gttttaaatg tctgtcaatc tctctatgag ggtaatggta agggtggata cataacttac
    11521 cctagaactg attccatttt cttagaagaa agtttggctt caaaagcttc tcaaacttta
    11581 gataaattaa aggttggtct tgaatatgaa aataaaatta aattttctaa aactaaaaga
    11641 gtttttgatt cttctaaagt agactcccat agtgctataa ttccaactta tataattcca
    11701 aaaagtttat ctaaggatga acaattagtt tatgatgcca taaaagatag atttgtagca
    11761 aactttatgc ctcctgctga atatgagaat acagaaatta agactgaagt agataattgt
    11821 actttcttaa caaaaggtaa agtgcttaaa tcaaaaggat accttgaggt atataataag
    11881 gaagaaaaaa acgatttact tccacttgta aataaagatg atgttgtaga tgtgttagaa
    11941 ataaaaccat taactaagca aactactcca cctaaaccat atactgagga caccctttta
    12001 aaggctatga aaaattgtgg taaaaatgtg cctgaagaag atacaacagt gctatctgga
    12061 tattcgattg gaacatcagc cacacgtgct gatgtgttaa agaagataag tcaagtgggc
    12121 tatgtaaaga aaaaagggaa atcttattct ataacagacc ttgggaaaaa tttagttgaa
    12181 atttttcctg ttaaagactt atttgatgta gattatactg gaaaacttga aaaaagttta
    12241 agcgatattc aaaaaggaaa gtatacaaga aaagaatatc ttactaatat cattagtttt
    12301 atatatcaaa atgtcaatat tataaaacaa gatgcttcaa aaaatataaa cacagaagca
    12361 tatactttcg attcaaaaac taaaaaattt actaaaacaa aagaaaaaaa acagaaaaaa
    12421 gatgtaacta atactgaaaa agaagttgct atatcaaaag attctaataa caaaaaagaa
    12481 gttaaaaatg gtgatgaaaa tcaatctctt ggcaaatgcc ccatttgtca aggtgatgta
    12541 ctagaatttg acaaaggctt tgcttgtaaa aatcataaag aatgtaaatt tgttatttgg
    12601 aaaaatgata agtatttaag tctttataaa aagaaagtta acaaaacaat ggtcaaaaat
    12661 attttaaaaa aaggtgaaac taaagtaaaa tctttaactg ctaaaaacgg taataaattt
    12721 gatgctacat taaaatatga taaaaatcca gataccggct atttcaattg gaaaatagag
    12781 tttgacaact aaatattcat aagaaaagaa atagcagtag tataataatc tatattatat
    12841 tactgctatt tcttttctta tgaagtttta acttttttaa tagtgacttt tacaatcaca
    12901 ctctaaccta atttctttac tatttctgat aactttaatg tatgttcatc taataaagtt
    12961 agttcctctt cagaaagatt agataatttt tttactagag attctttgag aatagctttt
    13021 actgcatcaa ataatttaaa tgctttatct gttaattcaa ccctaagaat ccttctatct
    13081 tttgggtcag gaaatttatt gactaaacca tgttccacta atctgtcaat aatcggagtc
    13141 atatttgatt ttaaaattcc taaattagaa gctatttgag aaattgatga tgaatttgat
    13201 gtcataagat aaagaattac cttagcatgt gatggagcca ttggacattc ttcaaaataa
    13261 gctttcatta tatcagagtt attttgaaac ttttttaaca aatcgctttc tttaaatata
    13321 ttattttcaa gcacaaataa aaaatctaaa aaagagtccg ctattttaag tatattgttg
    13381 ttatccatat ttgtaacatc ccttcaatag ataatataat aaaacattgt tgtttaatat
    13441 agtataaatt ataaactatt aataaattta aaacaagaaa aagttattga ctcataataa
    13501 ttataaattt ataatcaaat tagagaatta taaattgcca ataaagggaa gttttttaat
    13561 tagtctaaaa ctaaacttaa attataagac actttaaatt ctacaacaag ctagtgtgca
    13621 atatataata ggaggagata tgaaaagata tttaatagtt ttcttaatgt gtagttcact
    13681 gtttttagtt ggatgtggaa aaacaaaaga atctgaacct gagaaaccaa tagcagtttc
    13741 tgttcaaaaa gcagtaggtg gagaaattga aaacacaaat tctttctcag gaactacaaa
    13801 agttaaagat gaaacagctg ttactgcaca gactgttggc acagttcaag aagtgtatgt
    13861 aaaacttggg caaaatgtaa gaaaaggaga tgaattattg agtataagca gtccagagct
    13921 agaaaatagt gtaaagcaaa gtaaagcctc actagattta gcaaaagcaa gttattcaag
    13981 tgccactggt ggttcattag aggcacaagt aaatcaggca aaaacagctc ttgacaatgc
    14041 aaagattcaa tatgaggagt ctcaaagaaa ttatgacaat aataagattc tttatgaaca
    14101 ggaagtcatt agtttagacc aatttaaaaa aatagagttt tctttagagc aaacaaaaca
    14161 acagttagat tctgctcaaa gagcatacga tacagcaact tcaaaaagta tacctcaagc
    14221 taaagcactt gctaaaaaac agcttgacca agctcaagta tcatataatt tagcaatgag
    14281 taatttagac aaattaacat taacatctcc agtagatggt acaataacag ctaaaaactt
    14341 tgattcaaag gaaatgataa cacaatcaca accagctttt attatttcta atcctaatat
    14401 actagaagtt gatttaaatg tagcagaatc agatataggt aaatttaaga aagatggaaa
    14461 tgtagatgtt ataattgaag accaaaggat tttaggaaaa atagattatg taccttcagt
    14521 agttgaccct cagacatctt tatatcctgt taaagtactt gtaaataatg caaacaacaa
    14581 atttaaggca ggtatgtctg cacaagtaaa cttaagtatt gaaaaagaaa atggtgctgt
    14641 tacagtgcct aaaaaagcaa tatttgaaga aaatggtaaa aaatatgtat acatagcaac
    14701 aaaagacaat atagcaaaga aacacttggt tcaaactgga attgtaacag aggataaaat
    14761 agaaattaaa agtggtgtaa gtgataaaga tacagtgatt attggaggaa taagtcttat
    14821 ttctgacgga actaagatat ttcctgtaga aaaggagaag tagactatga atttaacaca
    14881 gacttccgtt aaacgtcctc tcacaatcat aatggtcttt ttagtattaa tagtttttgg
    14941 tggtattgga tataaaaaaa tgtcaataaa tctaatgcct gatatagaaa taccagttgt
    15001 aatggtcatg actacttgga caggtgctgg tcctcaagac gttgatgaac aagttagtca
    15061 aaaagttgat gaaagtttat ctgccgtttc aaatgtaaaa tctactatat caagttcaca
    15121 agaaagtgtt tctatggtag ttgctcagtt tgaatttgga actaatttgg atgaaataat
    15181 gaatgatgtt aggtctaaag ttgatgcatt acagaccagc ctcccagatg atgcagcaaa
    15241 accaacagtt ttaaagctgg atatgaatgc acaggctata ggtcaacttg tcatatctgg
    15301 aggaaatgaa aatagttcac aagccttaag aaaatacgct gaagatgtaa ttcagcctaa
    15361 aatagaatcc atagatggtg ttacttcagc agaccttaaa ggtggagaaa aagcacaggt
    15421 taatgtaata gctgacccag ctgttctttc aaattatgga gttagtttat ccactataaa
    15481 aggtgtctta tcctctagta ataaaacatt cccttatggt tcaattactc aaggtgagga
    15541 taaaatagtt ttaagagcaa ttgataaatt agaatcccta gatgatataa aacagattca
    15601 aattcctgta aaaggaggaa atacagttag attagataat ctttgtagtg tagattatgg
    15661 atatgcagac aaagatagta tttatagata caatggaaaa gacagtttgg taatagatat
    15721 ttctaaacaa caagatgcca acactgtaaa agttatgcag agtgcataca aatacgtaga
    15781 tgaattgaat aaggaaaatc ctcaatttaa aattaaaatt gctaatgata caagttcata
    15841 cataagtgaa tcaataaaca gtgtaatgag caacttgctt ataagtgctg ttatcgcatt
    15901 tattgtaata tttgctttcc taaaaagtgt aagagcttca ctagttgttg cagttgcaat
    15961 accaatttct ataataggtg ctatagcaat tctctacttt actggtgaaa gtttaaatct
    16021 tattactgca agttcattgg taatttcagt tggtatggtt gttgataatg ctacagttgt
    16081 aattgaaaat atatttaaat ataggaaaag tggcagattg agtcttgatg attgtgctat
    16141 tgagggaaca agaactgtaa caaatgcaat acttgcatca acattgacca cgatagcaat
    16201 attcttacct atcatgttta cagaaggtat agctaaaata acttttgggg cattaggtaa
    16261 aacactaata gcttcactag cattctcctt tataggtgca attacactcg ttcctagtgt
    16321 atttgcaaaa ttaagcagag gaaaaaatag tcaaaagatg caagaaaaag atagtcctat
    16381 atttgataag gtaagtgaag catataaaaa attattaagt gtatgtttaa gacatcaacg
    16441 cttagttgta atatcaagtt tacttatttt tgtagcttcc ttatttggaa tgggatttat
    16501 aggtatggac tttatgcctg cagcagacaa gggtgaatta tctatttcta tagaacttcc
    16561 aaaaggtctt agcctagaat ccaatgatta ctatgtatct atgactgaga aaaaagtctc
    16621 agatattcca gaaattaaaa ctttaataac aactttgaca tcagattcaa ataatagtaa
    16681 agcaagtata gcaattgaat tagaatctca aaaaaataga aaaagaacta ctgatgaaat
    16741 agaaaaagag ataattgata gagtggctac tgtaccagat tgtaaaataa atgtaagtca
    16801 aaacagtagt gttatgggtg gagctggagg agcagacttc acattagaaa tgaaaggtcc
    16861 tgatttagat gtattaaaaa tactgggaaa agaagctgaa aaagaattta gctctataaa
    16921 aggatttaga aatgttgaaa cttctcttac agatacaaca caagaagctc aatttactat
    16981 agataaacat aaggctcaag aatatggtgt aaatactgct gaaattgcag gcatgttacg
    17041 tacagcaata aatggtgact ccgtaaccac tgctactatt gatgattaca aagtggatgt
    17101 aaacctaaaa tttaaagcag atactattga tagcttagaa gatataaagc aactaaaagt
    17161 aaactctgca actggaaaag aagttcctct tggtgctttt tcagatataa aaatggcaga
    17221 tggtctacaa tctatatcta ggactgatgg agatttttct gtaaatataa ctgctacact
    17281 tgacaattta gatgctggta ctgctacaaa actagcacta gaaaaaacga ataaaatggt
    17341 aattcctaaa gattactcta taggtgttgg tggtgaaaca gaaatgatga atgaatctat
    17401 ggcaagtctt ggattttcaa tggtcatagc tatagcatta gtttatatgg taatggtagc
    17461 tcagtttgaa tcatttagta aaccatttat aatcatgttc tgtgttccat ttgcattcgt
    17521 tggagttgtg ctttcgcttc tagttagtgg tcaaagttta agtactgtcg gtatgctagg
    17581 tataatctta cttatcggta ttgttgttaa taatggtata gttcttattg actacataga
    17641 gcaacttaga aaaacaaatc gtaacaatga tttaattggt ttggtttcaa ttggttgtgc
    17701 agcaagatta agaccagtct taatgactac agcaacaaca attttaggta tgttgccaac
    17761 agcactatct cttggtgaag gtggagaaac tatgcaacca ctagccatag ttataatggg
    17821 tggtctaact gtatctacac ttataacttt ggtgcttata cctactatat acttaatatt
    17881 tgataaatgg gaaaataagt tttcagacaa aatgcctaaa atatttggta agataacaga
    17941 taaaatagga agtataaaac gtaaaataat aaagaaacca aaattgaaag ctaaagttta
    18001 aatagaatgg agggtgattg tattgaataa aaaaatgagc aaaatagttg caattggctg
    18061 tggtgttggt ataataactg ccaatttaat cccagtttac gcagataata atacttcttc
    18121 tataaacaac gttgataatt ctattttaag tgactcaaat aaaagaacat ctgaaaagga
    18181 aattttgact gctagagaag ctgttagtgc tgcacttaaa aatagtgaaa aattaaaaat
    18241 gaaatcagaa gaaataaaaa tgttaaaaga aaagttagaa gtacaagatg agtttgatag
    18301 ttttactgga agtgacaact cgtttccata cgaccaaatt ggacttctta aaaatcaaag
    18361 tgaacaggct aaaggcttta tggaggacca aatagcaaat gatattacta ataaatttaa
    18421 cgatttagtt tcaagagaaa atgagctaga taagataaaa aacaatcttg aaataaaaac
    18481 taaagaaatt aaagatatga aattaaaaaa agatttaggg ttagtaacat ctttagaaac
    18541 tgaatcagct gaacttgaac ttcaaacact acaaaatact caaaaagcta aacttcaaga
    18601 actaaaaaac aatcaagact attttaagtt attgactaat atagatttaa ataattatca
    18661 attggataaa gaatatagat ttgaatcttt tagagtaggt ggctcagttg attcatatat
    18721 ggaaggtaag gttaatgaat atttaaaata cgaccaattg attttagaac gtacagaaga
    18781 aagctttaat gataaagatg agaacaaagc agatttacca gatagaccta atcttacaag
    18841 accagttgct cctacaaaac cagagcaagg taacctaact gatgatgaat acaaggttct
    18901 tatggataag tatgaaaaag actacaagag atatgaaaaa gaagttgaag catacaatct
    18961 tgaaagacag acttatgctg ctggccttac tacttatgca aattatcttc aacaaaaatt
    19021 taataccgaa aatggtctag taacattaga agacagtaaa aaagctctta aaaaagggtt
    19081 aattgatagc tatgctcaat tacttgcttt agaagataca attcagatta ctaaaaaaca
    19141 acttgattta tcagaaaaac aattaaaaaa tactaaatta agatatgatt taggattaat
    19201 tactctaact gattataaaa aacaagtggt aagtaatcaa gatgctaaaa atagctatga
    19261 cactcttatt gtgaattata actcactaaa aaatggtata gaaaaacctt ggatacttaa
    19321 tactggaaaa taattctggt aaatagaaag attactttaa catacgtttg attaaaaaat
    19381 agttatgcac actcgtggct ttagtcatgg aagattcaga aggcaaataa tagaaataga
    19441 ttagtaaaaa tatatttact tataaaactg tatactttta tagaggctat ctcaaaattg
    19501 gatatgtccc tgtcaagtag acagacttaa aaaaggctat ctttatgcgg aatgagttcg
    19561 atattcaatc gaactcattc cgttttttaa ttgtattctt tcattattat aaaaataaat
    19621 ataatcattt attagactaa tcatttcctt tggatctgta gtatcaatta aatatattaa
    19681 ttcagattta aaatgactga aaaaactttc tataggagca ttatcccagc agttgcccct
    19741 tcgggacata gattgtataa taccattatc tttaagcctt ttagagtaag atctacttgt
    19801 atattgtgaa ccttgatctg tatgtaaaat acaatcttta gctaaattca tatttattag
    19861 ttgatttact gttttattaa ctaatttagt atccattttt gtacttatct catatgccac
    19921 tatttcacca ttaaataaat ctttggctac att
//
LOCUS       DAELXX010000073        19292 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.073, whole genome shotgun sequence.
ACCESSION   DAELXX010000073 DAELXX010000000
VERSION     DAELXX010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19292
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.073"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            287..865
                     /locus_tag="KPH29_002471"
     CDS             287..865
                     /locus_tag="KPH29_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG1468057.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            904..1482
                     /locus_tag="KPH29_002472"
     CDS             904..1482
                     /locus_tag="KPH29_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG1468058.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLRHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            1568..2197
                     /locus_tag="KPH29_002473"
     CDS             1568..2197
                     /locus_tag="KPH29_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG1468059.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            2187..4946
                     /locus_tag="KPH29_002474"
     CDS             2187..4946
                     /locus_tag="KPH29_002474"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBG1468060.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     PKKTA"
     gene            5033..6595
                     /locus_tag="KPH29_002475"
     CDS             5033..6595
                     /locus_tag="KPH29_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HBG1468061.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGVIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDIHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            6596..7732
                     /locus_tag="KPH29_002476"
     CDS             6596..7732
                     /locus_tag="KPH29_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBG1468062.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            7796..8872
                     /locus_tag="KPH29_002477"
     CDS             7796..8872
                     /locus_tag="KPH29_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HBG1468063.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNSHSITGILIPKANSELIENCIEALYSMNLQDMYVI
                     PIIETREFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            8850..10088
                     /locus_tag="KPH29_002478"
     CDS             8850..10088
                     /locus_tag="KPH29_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HBG1468064.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGVRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELNCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFAKEKENQYDIYYHST
                     TRSPIINIDKKHYPIKSKFMLKSFYNIDIQNYIYNIDKHNFSKCYLFVELNKSENSYL
                     EFIDIIKKTSIKELTIVCCS"
     gene            10142..11230
                     /locus_tag="KPH29_002479"
     CDS             10142..11230
                     /locus_tag="KPH29_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HBG1468065.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNVSLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKHFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLIGKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            11258..12049
                     /locus_tag="KPH29_002480"
     CDS             11258..12049
                     /locus_tag="KPH29_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HBG1468066.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYANIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKPDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDFSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFKGMSGTEEILSNILDENCLLNI"
     gene            complement(12164..12718)
                     /locus_tag="KPH29_002481"
     CDS             complement(12164..12718)
                     /locus_tag="KPH29_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBG1468067.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTQKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            12913..14115
                     /locus_tag="KPH29_002482"
     CDS             12913..14115
                     /locus_tag="KPH29_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HBG1468068.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            14172..15152
                     /locus_tag="KPH29_002483"
     CDS             14172..15152
                     /locus_tag="KPH29_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBG1468069.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLTEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
     gene            15399..16352
                     /locus_tag="KPH29_002484"
     CDS             15399..16352
                     /locus_tag="KPH29_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBG1468070.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RVPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAVFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            16342..17298
                     /locus_tag="KPH29_002485"
     CDS             16342..17298
                     /locus_tag="KPH29_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBG1468071.1"
                     /translation="MKLSLNQKIYFVSILLFISIALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKMLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            17295..18050
                     /locus_tag="KPH29_002486"
     CDS             17295..18050
                     /locus_tag="KPH29_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG1468072.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRSLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVEKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            18142..19107
                     /locus_tag="KPH29_002487"
     CDS             18142..19107
                     /locus_tag="KPH29_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG1468073.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTIKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAENLVGLPKTSLPASLEKYKDKKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
BASE COUNT         7645 a         1951 c         3432 g         6264 t
ORIGIN      
        1 aacttaaaac aatatagtac aataattttg tagtttcaaa ataaaatatg tacttgttta
       61 tataaatgat atataattac atattaaaca tagcttttat acatttaata aagtatgtaa
      121 aatctatgta aaaagtaaga aatataaaca tttttttaat tatattttaa aaatatcaag
      181 caagttttct tcacaaatga ataaaaaatg ttataatatg attaaagaaa aagataaata
      241 attagcaaga tttgtttagc taaaattaac aagggagcga taaattatgg gtataacatt
      301 atcaaaaggc caaaaggttg atttaactaa aggaaatcca ggtcttaaaa atattttagt
      361 aggtttagga tgggatacta acaagtatga tggtggattt gactttgatt tagatacagc
      421 agcattttta acaggagcaa gtggaagtgt tactaatgat ggagattttg tattttacaa
      481 caatttaaaa catacatctg gagcagttga gcatctagga gataacagaa ctggcgaagg
      541 tgatggagat gatgaacaaa tagttgttga tttatctaag attcctggag aaataagcaa
      601 gatttctttt actgttacaa ttcatgatgc gacagaaaga agacaaaatt ttggacaagt
      661 aagtaattct tatataagaa tagtagataa agacacaaat gaagagttaa taaagtatga
      721 attaggagaa gattttagta tagaaacagc tatagtagta gctgaaatat ataagcacaa
      781 tggagaatgg aagtttaatg cactaggttc tggttttgaa ggtggtcttg ctgcattatg
      841 tggaaacttt ggaataaact tataatttca actataacaa taaactaaag ggggatattt
      901 tccatggcta tagtattaaa aaaaggacaa aaaatcgatt taactaaggg aaatccaggt
      961 cttaaaaata taaaacttgg actaggatgg gatacaaatt catttgatag tggctatgat
     1021 tatgatttag atgtaagtgt tttcatggtt ggagaatctc aaagagttga aaaagatgaa
     1081 gattttatat tctacaataa cttaagacat ccatctggag cagttgaaca cttaggtgat
     1141 aatagaactg gtgaaggtga tggagatgat gaggaaatat tagtgaattt agagacaatg
     1201 ccaaagcata tacaaaagat agctgtaaca gttacaatat atgaggctgc tgaaagaaga
     1261 caaaattttg gacaagtaag caactcatat ataagagtat taaattctga aaatgatgaa
     1321 gaaatattaa gatatgactt aggtgaagaa tttagtatag aaactgcaat tacagtatgt
     1381 gagatatata agtataatgg agaatggaag tttagtgcag ttggagcagg atttgaaggt
     1441 ggcttagaag cactttgtag aaactatggc ttaaatgttt aatttaagca atataatata
     1501 ttaaatataa ataacctata caaataatgt ataggttata ttatataaaa ttttgtggag
     1561 gtttagtatg tcgattaatt tatcaaaagg tgataaaata gatttaaaaa aatctaaccc
     1621 aggactgagt aacatacttg taggtctagg atgggaccct gtgcaacaat ctggaggagg
     1681 tttttttaaa tcgttatttg gtggaggaca agctgatata gattgtgatg catctgtatt
     1741 tatgcttaat caagaaggta aattaagtgg aataaaagat ttaatttatt ttggaaatct
     1801 aaaaagtgcc tgtaaatcag ttttgcatac aggagacaat ctaactggtg agggtacagg
     1861 tgatgatgaa caaatcctag ttaatttgga taaagttcca tcaaatattc ataaattatt
     1921 gtttgtagta aatatataca attgtgtaga tagaaaacaa cactttggaa tgatagaaaa
     1981 tgcctatata agagttgaag accaaggtaa taagaaagag atagcaaaat acaatctatc
     2041 tgataattat tctgaaaaaa ctactcttat agtaggagct atatatagaa aagatggaag
     2101 ttggcaattt aaagcaattg gtgaaggaac aaaagatgcg ggtcttaaag aagtaatgca
     2161 aaatttagac aggattgagt gtgcatatgg aatttaataa tgatatcaaa tattataatg
     2221 tagataaaga agaagttaca aaagacttat ctgttaatcc tgaaaaaggt ttatcagaat
     2281 cagaagtcaa gactagaaga gaaaaatatg gtttgaatga atttactcca aaagaagagg
     2341 gtagtttttg ggatgattta aaagaaagtt taagtgaacc aatgatagtt atcttgatta
     2401 ttgcagcagt agtaagtgca gtaattgggg aaactcatga tgcaataggg attgtaggtg
     2461 ctatagcaat aggtatagca ataggtatga ttacagaagg tcgttctaag aaagcagcag
     2521 aggctctttc taagttgact gaaaacatag aagtaaaagt attgagagat ggggaagtac
     2581 atcaaatttc taagagtgaa ttggtaccag gtgatatagt atatattgaa actggtgaca
     2641 tggtacctgc tgatggtaga cttatagaaa gtataaacct taaaattaga gaagatatgt
     2701 taacaggaga atctgatgac gtatctaaaa agtgtgatgt agtagtttct atggaagaga
     2761 tagaatctaa aggagcagta gtagtacagg agccaatacc tgcaaaacag ataaatatgg
     2821 tgtttggtgg tacacttgta gcttatggta gaggagctct agttgtaact tcaattggag
     2881 ataatagtga aatgggtaaa attgctcaaa acttatcaga aacagatgag caaactccac
     2941 ttcagataaa acttggaaat ttaggtggtt tgatagccaa agtttctagt gcagtagcag
     3001 gattactatt tatatttatg gttttccaaa tgataagtaa gaatgtatta aatgtagata
     3061 tgtctggaat attaccgttt ttagaatcaa tagacccagt taaaacagca tttacagtat
     3121 gtgttgcgtt gatagttgca gcagtgccag aaggtcttcc aactatgata aatatgacac
     3181 ttgcaataac aatgcaaaaa atggctaaaa taaatgcatt ggttaccaaa aaagaagctt
     3241 gtgaaacgat aggttctgtt tctgtaatat gttcagataa aactggtaca ttaactgaaa
     3301 atagaatgac tgtagaagtt gcatatgttg atggcagata tattgaaagt tcagaagagg
     3361 aaataaatag ttattttgaa gaaaactgta tgataaactc tacagctgat gtagagcaca
     3421 atgatggtga tattaagtat ttaggaagtg ctactgaatg tgcattgctt ttatattaca
     3481 aaaacgtaga ttatagacaa gttagaaaaa attctaatat agtagctcaa aatcctttta
     3541 cttcagattc aaagagaatg acttctgtta taggtcaaga taatcatcat gtactattat
     3601 caaaaggagc accagaagtc ttacttgaat tatgttcaca tattcaaaga ggaaaagaca
     3661 tagttccatt aactgaaaat ataaaacatg agattttaga agaaataaag aaattgcaaa
     3721 taaaatcaat gagaacttta gggtttgcat ataaagaaat ctcacaagct gaagaagaag
     3781 cagcagtaac tgctgaaagt gatgctatga atgtaagtgc aatggaaaat aatttagtat
     3841 ttagtggttt tgttggtata agagacccac ttagaaaaga tgttatagag tctgtaaata
     3901 cagcaaataa agctggtgtt tctgtaaaaa tgcttacagg agataatata aatacggcaa
     3961 gagctatagg tgaagagtta ggcctattaa aaaataatat gagagtggtt gaagcttcat
     4021 atatagatac tttagatgat gaagaattaa aacaagagat acaaagtatt tcaatcgtgg
     4081 caagaagtaa accagatagt aaaatgagaa tagtttcagc tctgcaaaag agcggggatg
     4141 ttgttgcagt aacaggtgat ggtataaatg atgctccagc actttcaaaa gcagatgttg
     4201 gtatagcaat gggtatatct ggtacagagg tatctaaaaa cgcagcagac ataattttaa
     4261 ctgatgatag ttttagtacc attgtaaaag gtattaaatg gggtagagga atttatgata
     4321 acttccaaag attcgttcaa ttccaattaa ctgtaaatgt aattgcattt ttagtggcta
     4381 taatatctca agttatggga caagagatgc catttactac aatacaatta ttatgggtaa
     4441 acataattat ggatggacca ccagcactag cattaggttt agagccagtt agagaccatg
     4501 tgttaaatag aaaacctgtt gatagaaaag ccaatataat ttctaagtca atggtgtata
     4561 caatagtatt aaatgcattt tatataacag caatactaat gttacaatct acatttaatt
     4621 tcttaggagt tgatgaagct cataagggaa cagtaatgtt tgcgttattt gcttttagcg
     4681 cactgtttaa tgcatttaac tgtagagagt ttaatgcaga tagtatcttc cctaatttta
     4741 cacataacaa attagcacta caaataatag tgttgacagg aattgcacaa gtaatattta
     4801 ctcagctatt ccaagatttc tttaactctg tagcaatgga ttttatgaca tgggtaaaag
     4861 ttttatgtgt agcagcaagt gtaattgtgg ttaatgaagt tgtaaaatgt ataataagac
     4921 tggtaaaacc aaaaaagact gcttaaaaca aattttttag aagaaaacag actgactcaa
     4981 aatatgaatt ttgggtcagt tctgaataaa catttaaatt gggaggatag ttatgggtga
     5041 ctcaaaggta tttgaaaaaa tattctccag ccaatcagat agaggaaatt acactccttc
     5101 aaagggatat ctaagttatt ttatttcgta tattggttta gaagatgaag ttttgtacaa
     5161 tctcgagata tttaagacaa aacaaaatat tgatagcaaa aaagatattg ctttatttac
     5221 tgatgttata gcaaatccta gtgattttga tattataaat tattttaaaa gtggacttca
     5281 aaaatataga actagcatgg aagatgtaga tataaatatt ttgggatttg aagagattga
     5341 ttataaaata aagcaagcaa tggatagagt gttaaaagaa gaggaaaaag aatttactaa
     5401 tgatagagtt aaacaaaatt ttatagtgaa aataatggca tggataaaaa tttatatcgg
     5461 tgctttagat ataaataaaa atgaagctcc aaaagtaata ttttatggag atataaaaaa
     5521 acatgaagtt tatttactct taatattata cttggcaggt tttgatgttt tatatcttaa
     5581 tccaaattca aagagtaata tagatatttt aaaatcagaa agatacaata tagagtttga
     5641 agaagccaac ataatagaag aaaaaatttc ttttgaagaa agggttattt taggggaaaa
     5701 aatagataaa agttctgtaa aaaaagcttt tactgttggg gcagaagcat caaagcgtat
     5761 aagtgaggaa ttgttaaatg atgcaggatt tataaaacct tggcaactcc aagatagaaa
     5821 aataaaaaat ctacttctaa gttcaactgt tgatgaaata tcaatatact ggaatcaacc
     5881 acttaaatta agaccaggat ttaagtttaa tgatgctatt gtagaagctc caaattttct
     5941 ttctaagatt aatggaatat ataatgataa gaatgaatat attaagtttt tagatttatt
     6001 aagagattcg gaaagttcaa catttattga atttaatgga gatgtagata gattttcaaa
     6061 agcttttacg agagaagctt ttagtttatc ctttttattg gattcaaaag gtgtaataga
     6121 taaaaactct gttttaaata ataaagatta ttccatatca acactagcac ttaatcaaca
     6181 aattatgatt ttagagaaag tggaagagtt gttagaagga agtatgtttt tgaatggttt
     6241 aagtggagaa gacaagataa aaggtctatt tacagtactt cacatggata agaaatttgt
     6301 tcatatgatg aacaatttcg attactcact tataaaccca aaattgatta tatacatgta
     6361 caagagtatt gtatttgata aagaaatagt ttttcttatg cttttactta gtaagattgg
     6421 atttgatatt atcatattat gtccaggagg agaaaacaat atagagaatg ttataaataa
     6481 ccaattgatt gatatacaca gattggataa aatggtatat gaccttaaat taaattcgct
     6541 tgaaaatgat atacctttac ttaaaaaaat ttttggaaag aggagacgat tttagatggg
     6601 gataaatata aatagttttt caaaagataa gaactctgta gaggttactg agaatttgcc
     6661 tacagcagtt caagaagaaa acttcgatat aatggaatat acaaataata aaaaaaatga
     6721 actgagaaag tctaaggaag ttgaggcatt gacatccctt atagaagtag aaaatccaga
     6781 tacgatttta cagtttggta ggaaagcatc tgaaggagta gcaagagttt ctgatagcct
     6841 tttaaataca ataaaattaa atagaaatga agaaaattct aaaatgctgg ttcatttaac
     6901 taaaataatg gataagtttg atttagacga ttttcaagaa actaaggaac caaatttcgt
     6961 tcaaaaactt ttcaaaaaag ctaataatgc cattgaaatg atgtttcaaa agtatgaaac
     7021 attgggagga gaagttgaga aaattcaaat tgagcttgag caatacgaaa gagatatagc
     7081 actttctaat aagcaaatag gtgctatgtt aaatgaaaac tttgagtttt ataatgaact
     7141 tcaaaaatat attgttgctg gagaaatggc aatagaagaa atggataatg aaatactacc
     7201 tttctttaaa caaaaatcag agactagtgg agaccaaatg gatgttgtca attatcaaga
     7261 actattaaaa gtttatgata tgttaaatca aagagtctat gatttaagaa tagcagaaaa
     7321 tatagctatc caaacaatac ctatgcttag aggtatgcaa cataataatt atggtttaat
     7381 tagaaaaata aatagtgcat ttgtcgtaac tttaccagta tttaaacaat gtttatctca
     7441 agctatatta cttaaaaagc aagaattaca agcaaaatct ctaaaagcac ttgatgacaa
     7501 aactaatgaa ttattgctta gaaatgcaca aaatgtaagt actcaaagtg cacaaatagc
     7561 tagaatggct ggaacaagtt ctgttcaaat agaaacattg gaaaaaatgt acaatactat
     7621 caagtctggt atagatgaaa ctatgagaat agaagaaaac aatagagctt taattaagga
     7681 taatacaaag agattggaag aattaaatac gactataatt tataataagt agtattatat
     7741 taagggctaa tttgtataaa gttggccctt tctatgtata tgaggaggct agacattgaa
     7801 atatttcgat tatatctgca aagatgattt ggagagaatt tttttgaagg agccagaaga
     7861 ctttagtgct aagactgaaa aagatgtgtt gaaatatgca ctgggcgctt ttttatacgt
     7921 tccagctact caatataata tgatttataa gagtattata ggggatgtta agggagttag
     7981 accattagct atttgtttag aagatgctgt tggagtaaat ggagagttag aggctataga
     8041 aaatcttaga ttaattttaa aaaatattag caatgaaagt ataactaata aagatggaat
     8101 accactaatt tttgtaagaa taaaagatgt ggagcaactg ttgagaatta aagagattat
     8161 aattaaaaat agtcattcta taacaggaat attaattcct aaagcaaatt cggagttgat
     8221 tgaaaattgt atagaagctt tatattctat gaatcttcaa gatatgtatg ttattccaat
     8281 aatagagaca agagaattta tatataatga aaaaaaagaa cttagtttta ctaatttgta
     8341 caatgctatt ttaagacata agtcaagaat attaagcatt agagtaggca taactgatat
     8401 attagggatg tatggtataa ggagagataa aaacttctct atatataata atttgatttg
     8461 ttcttcattt atattagata taatcacata tcttcaaaga ccagagttag atatacctat
     8521 aagtggtgga gtatcagaat tttttgatat gacaaataag gatataagaa ataagtatat
     8581 agaagaaata ttattggata aatatcatgg attaattgga aagaccgtaa ttcatccaat
     8641 gcaaattcaa attgtacaag cgctatctac agtatcatat gaagatttta ctgatgcttt
     8701 ggatatactg gatagtacag acagtaagta tggtgtgagc aagggtgtat taggagagag
     8761 aatgaatgaa acaaatccac attttttatg ggctaaaaaa acattgattc tatctaaaat
     8821 atatggtgta ttaaataaag gagtagacta tgaagaattg cttaaatttt aattgtgaga
     8881 ttgacattat tgataatcca ttgaatttag aactaaatga ttttttagac gtaggagtaa
     8941 gaaataatag caaaagacgt tttttgttta tctcaaagaa actcggtaaa catttagcat
     9001 gtaagccaag tgagatggat aatttgggaa aattaatggc tacaatttat aatgaaaaaa
     9061 ataaggaaga atcaaatggt actgttatag cttttgctga aactggtaca gctgttgggc
     9121 atagtttttt tgattactta tgtggagatt atgaatttat tcatactact agagagcagt
     9181 ttgacgagtt agaaaatctg gaatttttag aggaacattc ccatgcaaca gaccagaatt
     9241 tatactttgg tatgcttaac aactttaaat ttggagatga gataatattg gttgatgatg
     9301 agattaccac agccaataca tgcatgaata taattaggaa aattcaaagc atttatccta
     9361 aaaagaaata cactatatgc tcaatactca attgggtaag tgatgaaaat ttaagtaaag
     9421 ttactcgttt agaaagagag ctaaattgta agatagattt tgtatactta tttaaaggaa
     9481 actttaaatt ttatatagat gaagatatta tttcaaatta caacgataat tatagtatta
     9541 ctaaattaga gactatttca agtgataaag taaatttagt agtaaatcat atttatgttg
     9601 atttagaaga ttatattgga aataagaaat atgtaaaata tacaggaaga tttggaatta
     9661 atagaagaga acaatttaga ttaaaagaaa tcgttaagag agaaagtaaa aaaataacta
     9721 tggccagtaa agaatcttta aaaaatgaaa aagaaaagat tctattttta ggtactgaag
     9781 aatttatgta tatacctatg ctgtttgcaa aagaaaaaga aaatcaatat gatatatact
     9841 atcattcaac aacaagaagt ccaattatta atattgataa gaagcattat cctattaaaa
     9901 gtaaatttat gctaaagagt ttttataata tagatattca aaattatatt tacaatatag
     9961 ataaacataa tttttctaag tgttatttat ttgtagagct gaataaaagt gaaaattctt
    10021 atctagaatt tattgatata attaaaaaaa caagtataaa agaattaact atagtatgtt
    10081 gtagctaaag tgttttaaca ggataaaata tatcacgatt ttaagatagg agttgagtca
    10141 tatgagagat tttagcacgt attctaaaga tgatgttatt tttttattaa aagatatatc
    10201 aaatttaatt gaagaagaag gaaataaaga aagagaatta aaaatacaaa atggtagaca
    10261 ttattcagaa atgataccaa tagagtatga agttagtgaa gattatctga atttatatca
    10321 tgagaaacta agagaaaata aagataagct tgcattttct ataggagtta tgtgtgaaaa
    10381 aataattaaa aaaaatggaa atgatgtagt actagtttca cttgctagag caggaacacc
    10441 tattggtatt ttggcaaaaa gatatataag aagcaagtat aatgtaagtt tacctcacta
    10501 tacaatctct ataataagag ataagggaat agatatgaat gcaattgaat atatagtaaa
    10561 gaatcatccg agctcaaaaa ttcaattttt agatggatgg actggtaagg gaactatatc
    10621 aaaagaactt gaaaaagcat gtgatgaact taatgttaaa tttaaaaaac attttgactc
    10681 aacacttgct gtattagctg accctgctgg atattcagga ctttatggaa ttagagaaga
    10741 tttcttaata ccaagtgcat gtttaaattc aaccgtttct ggtttagtaa gtagaactgt
    10801 actaagagag gatttaattg gtaaaaatga ctttcatgga gctaagtttt atagacattt
    10861 aaaagataaa gatgaatcta tgaactatat cgaaactata gaagaatgtt ttaaaaatca
    10921 atttaagaat atatctgatg aagttgaaaa ctgggaaagt gacattataa caagagatgg
    10981 atactttgat gttttaaata taaaagaaaa gtataacata acagatatta actttatcaa
    11041 accaggtgta ggggaaacga caagggtttt acttagaaga gttccgtata agattcttgt
    11101 taaagactta aatgataaat ctttagacca tatattcata ttagctaagg agaaaaatgt
    11161 tgaagttgaa caaatggatt taaaagccta taaatgttgt ggaataataa aaaatatgaa
    11221 agatatttag gaaatatatt agacaggaga atattgaatg attgtttttt cagatttaga
    11281 caggagtatc atttactcca ataaattttt aaatgctgat agtaaatatg ctaatataga
    11341 aatttatagg gaaaaagaaa tatcttatat atctttagat actataaatt taataaagca
    11401 aatacaatat tatggtatgt ttatacctac aactactaga acagtggagc aatttaagcg
    11461 tatagaattt aataaatatg ggatttattt tccttggagt ataacttcaa atggaggagt
    11521 tatacttaaa gataatgaaa tattgaaatc atggagtgaa aaaatagata aactaaaacc
    11581 tgattatgag cccattgaaa gtatgattca taaatttaaa gactatttga atgtagatgg
    11641 aataactaat tttaaagttg cagaagatac atttttttat atagttgttg atttctcaag
    11701 atttaatttg gattcaatca aagaatatac aaatatattg gaatcaaaaa attggaagtt
    11761 ttatgtgagt ggcagaaaaa tatattttat accaaaagaa atttcaaaag aaaatgctat
    11821 aaaatacttg actaaagaat taggaataga atatttttat gcagttggag attctattat
    11881 ggactatggt atgcttaata tctcaaataa atcctatgta ttgaagcatg gtgatataaa
    11941 taaaaatgaa atagaaaatt catttatatc aagttctttc aaaggaatga gtggaactga
    12001 agagatatta tctaacattt tagacgagaa ctgcctactt aatatataat gactgatata
    12061 taaataataa actagaaaaa tctgaaataa attatagtaa agtttatcat actaatttat
    12121 ttcagatttt tatcaattta tatttaagta agaactcaat atgttaaaat gaaggtggag
    12181 tcattgccca gactgagata catacctcat catcataatt ttcatacaca tgtggtatag
    12241 tactgttaaa ataaaaacta tctccttcct ccaatagata ttccttattt ccaagtgtta
    12301 tcttcatttt accttttatt atatagccac attcttctcc ttcgtgtgtc acaaattcac
    12361 tgtttttttg tgtatgacca tctagcgtaa caagtataaa ttctattttt ttcccagata
    12421 aattaggaac aagaagttca taaaattttg tagaatcatt agttactatt ttttttcgat
    12481 tatttttctt tactactata tcagagtcct ctcttccaat ctcttcaaaa aaataaccta
    12541 tatttatatc aagagctttt gatattttcc acattgctac aatagatgga acaaccatat
    12601 tacgctcaat ttgacttatt aagcctgtac ttaaatctga attttttgct aaagtagaga
    12661 tacttatatt tttagatttt ctaagagatc taattttgct tccgacatct atattcatta
    12721 gatacaaacc ccctcagaca tgattataac atataattta taaagattaa taaaataaaa
    12781 aaaagtttta taatgcatgt attttttatg ttatattgaa ttttctgaaa agatgcatta
    12841 taatagtatt aatattcaat atattgaata ttgataatga tattttaatt taatgaaatg
    12901 gaggtaatta agatgaagag ttttgtaagt cctgataata tttgggtatt atgggcagtt
    12961 gtaactggat gggcagcatt tagtatttac ttggaacaaa agtacaattg ggcatcaaaa
    13021 gtgtcgggag caataatagc tcttgtagga gcaatgttat tatcaaatct aaatattata
    13081 ccagtagagt ctgttgtata tgaccaagta tggggatatg ttgttccttt agccatagct
    13141 ttattgttat atcaatgtaa tattaaaaag atatggaaag aaagtggaag attgctcatt
    13201 atatttcttg taggttctgt aggaactgta cttggagcta tgattggatt tttagcatta
    13261 aaaaatgtag taccagattt aaatattgtt gcagctatga tgacaggttc ttatataggt
    13321 ggaaatgtaa actttgctgc tatgtcaggg gcgtttgatg cgccaggaga attagtatca
    13381 gcaacagttg tagcagataa tcttttaatg gcattatatt tctttgtctt aatagctata
    13441 ccatcaattg gattttttag aaaacatttt aaacatccac atgtagatga aatggaaagt
    13501 ataggtataa ctgaaggaga aacagtagca gcaaattatt ggggaagaaa agaaatatct
    13561 ttaaaagata tagcaatagc cattggtagc gcctttataa ttgtcgcagt atctgtagaa
    13621 ctatcaactt ggtttaaagc tataatacca ttatcaaatc cttttttagc aatgttaaat
    13681 actttatttg gaaatcaata tctaataata actactttga ctatgttgtt tgccacattt
    13741 atgcctaatt tctttggtga tataaaagga gcacaggaat tgggtacatt tttaatatat
    13801 atattctttg ttgtagtagg tgttcctgct tcaataacat taataattca aaaatcacca
    13861 ttactacttt tatactgcgg aataatggta ctaataaaca tgctagttac atttatagtt
    13921 gcaaaaatat ttaagtttag tttagaagaa gctattcttg catctaatgc aaatattggt
    13981 ggtcctacaa cagcagcagc tatggcaata tcaaaaggat ggtctaagct agtagggcca
    14041 atacttatag ttggaacatt tggatacata gttggtaatt attttggtct tttagtagga
    14101 aatattttga tataatttta cgtgaaaaga agttgttttt tagaaagatt tttatttaat
    14161 tggaggttgt tatgattttc gatggacatt cagatacttg ggcagatgta acaataagaa
    14221 ggcttaacgg ggaaaaagat gtatttagaa gaatccatct tgataatttt aaaagagcaa
    14281 atatagaagg agctattttt gtaatatgga tagacccacc atatgataaa gagcctgaaa
    14341 aaagagttaa acaaattata agctctataa aagatgaaat tgaagatatg aaagatgttg
    14401 taaacatagt aaaaaaatat tcagatatag ataaaggtat aagtgaaaac aaaataaata
    14461 ttttaattgg tcttgaagga ttaagccata ttggaagaaa tatagattta ctagattatt
    14521 tttataaaga agttggtgca agacatgcat cattaacttg gaatgaacag aatgaacttg
    14581 ctacaggatg tttaggggat gaaaatcgtg gtttaactga aataggtaaa aaagcagtca
    14641 ataaaatgga aaaattgggc atgatagtag atgtgtcaca tttaaatgaa aaatcatttt
    14701 gggatttaat gtctgttgca agtaagccag ttatagcttc tcattctaat gctaggagtt
    14761 tatgtgatgc taaaagaaat cttacagata atcaattaaa agaaatttca aatacaggtg
    14821 gattagttgg aataaatagt tttagaggat ttactaatag taatcctaag aagcaagatt
    14881 taatacattt ggttgaccat atagactata tggtaaatct tataggtata gaacatgtat
    14941 cttttggttt tgattttagt gattacttag ggaaagagac tataggttct tttactggaa
    15001 atgctagtgt gccaagtgta gatggtcttg aaagcacaca tacagcacag aacatagtag
    15061 tagagcttga aaatagaggt tataaaaaga aagatataga gttaatctct agtggtaatt
    15121 tttataggct ctttaaagat atactaaaat aatataataa agaacttaaa tttataaaat
    15181 agtatattta tacaataata tctacatatt taaattggcg ttcataattt attaaattaa
    15241 attttaatgt tactagaagt ttcaattcta gtaacatttt ttatgttaaa aatagatata
    15301 gatatatatt gacaatgata attattattg ataaactata aaagtgaaaa tgataataat
    15361 tttcatttta attaataaaa gatttttaag gagggagaat gaagaaaagg tatttagtca
    15421 taggaataat aattttgtct tatttttctt tatttatagg agctgaagat ataaatataa
    15481 tgcatatatt tgcaaaaaat cagcataagc ttatgatatt tatcatgagt agggtaccaa
    15541 gattaataag tatattgatt gctggtgtag gaatgagcat agcaggtctt ataatgcaac
    15601 agataagtaa gaataaattt gtatcaccta caacaggtgc tacaatagat gcagctcaat
    15661 ttgggatagt aatatgtatg cttttagtac caactgcctc tatttttaca aagacaataa
    15721 tagcatttgt attttctctt gtagggactt ttacgtttat gaaaataata gggaagttac
    15781 aatttaaaaa tataatcttt gtaccattag taggtataat gtttggaaat ataataggtt
    15841 ctatgacgga ttttatcgcg tataaatacg atttgagtca aaatgttagt tcttggatgc
    15901 aaggtgactt ttctatgata cttaaaggaa attatgagat attatatatc acaatacccc
    15961 ttataatatt agcatatatt tatgcaaata aatttactgt tgttggtatg ggtatggatt
    16021 ttgcgacaaa cttaggtctt agttacaaaa gaattgtcaa tataggtctg ataatagtag
    16081 cacttgttac agtatgtgta gtcgttacag ctggtaatat cccttttata ggtctaatag
    16141 ttcccaatat cgtatccttg tatatgggtg ataatataag agcaagcata tggtatactg
    16201 gtttacttgg agcggtattt gtacttatat gtgatatttt tggaagaatt attatttatc
    16261 catatgaaat atctatagga cttacagttg gagttatagg aagtatacta ttcctttatt
    16321 taatattaag gaggaatgtg aatgaagctt agtctcaatc aaaaaattta ctttgtaagt
    16381 atattactat tcatatcaat agcacttttt ataatttatg gaatagacat gaatcattta
    16441 gaatatgcat tatcacaaag aataccaaaa atatttgcta tggtattagg tggaggatgt
    16501 atagcattta ctactgtagt gtttcaaaca attacaaaca atcaaatatt aacaccaagt
    16561 gtactaggtt tagattcatt gtatgtaatg attcaaacta taatagtatt catatttggt
    16621 tcaagcagta gtctgattat aaatgaaaat tataatttta ttattaatgt agctatgatg
    16681 attggagctt cattgttatt atataagatg ttatttgaaa aaaataaaaa taatattttc
    16741 tttttaatcc tagtaggtat gatatttgga accttattta aaagtgcaac aacatttatt
    16801 caagtaatga tagacccgaa tgaattttta gctttacaaa ctagtataat ggcaagttta
    16861 aacaatataa acactaatgt attgttaata gcatttataa taattattgc cataatacct
    16921 tttatatatg atgaaataaa gtatttagat gtattgtcgt taggtaaaga acaagcaatt
    16981 aatttaggtg tagattttga taaggcagtt aaaaaaatga taatattaat agcaatttta
    17041 gtctcaatat caacagcact tattggacct atgacatttt taggactttt acttgctaat
    17101 ataacaagag aaatttttaa aacatataag catacatact tgatttcggg ttctatgttg
    17161 atagggatga ttacattgat aataggtcaa ttttttatac aacatgtatt taagtttgat
    17221 acgactttaa gtgtagtaat aaattttata ggaggaattt actttattca attactatta
    17281 aagggggcaa atagatgata gagataaaaa atatatttaa aagatataag aataaaaatg
    17341 ttgtagatga tgtctctttt agtatagaaa agggaaagat aacatctttt ataggaccaa
    17401 atggagctgg aaaaagtaca gtactatcaa tagttacaag gcttatagga ggtgatggcg
    17461 gagaagtaat aatagaagga aaaagcttga ctaattatag caataaagaa ttagcaaaaa
    17521 aaatagctat tcttaaacaa tctaacaata taactttaaa gcttacaata agagaattag
    17581 ttggatttgg aagatttcca tatagtgaag gtaatctaac taaagaggat gaaaattata
    17641 ttgatgaagc aatagaatat atgaagttga ctgatataca acataagtat ttagatgaat
    17701 tgagtggagg tcaaagacaa agagcttata tagcaatggt aatagctcaa gatacagagt
    17761 acatattact tgatgaacca ctgaacaatt tggatatgaa ccattccgta cagatgatga
    17821 aggtacttag aagtctttgt gatgagttag ataagactat agtattagta atgcatgata
    17881 taaattttgc atcatgttat tcagataata tagttgcatt aaaaaatgga aaagttgaaa
    17941 aagtaggtcg tacagatgag atagtaaatg aaaaagtttt agaagatatt tatgagatga
    18001 attttaacat taagaatata aatggaaatc gaatctgtat ttatttttaa aatattgtat
    18061 atgaaaataa gcttgctata tgatttttta taaagttttt aaattaaaat aaagattaat
    18121 aggagagagg aaaatattga tatgaataaa aaagcagcaa tagtagcagc agtagctatt
    18181 ataggattag tgacagtgtt tgcattaggt ggaagtaaaa agaatgaatc aaaaacatca
    18241 gaaaattcaa ataataccat aaagataact cataacttgg gagaaacaga tgtaaaatta
    18301 aatcctaaaa aagtagtagt atttgattat tcggctttag atacaatgga tgcattaggt
    18361 gtagctgaga atttagtagg acttccaaaa acgagtttac cagcatcttt agaaaaatat
    18421 aaggataaga agtatgcaga tttaggaggt ttaaaagaac cagatttaga aggtataaag
    18481 tcggcaaatc cagatttaat tataataaat ggaagacaag aagattttta tgaacaattg
    18541 tcaaaaatag caccaacaat aagtacaagt aaagatgata agaagtactt agaatctgtt
    18601 aaaaataata tagacaaaat agcaaaaata tttggagtag aagaaaaagc aaatcaagaa
    18661 tttagtaaaa ttgaaaagaa aatagaaact ttgaataaaa aagtaacaga taaaaattta
    18721 aatgctttaa ctataatggt aaatgaaggt aatttaagtg tatttggtga agaatcaaga
    18781 tttagtatat tatacaatag ttttggattt gaaaataaag ataaaaatat aaaagaatca
    18841 agtcatggac aaaatattac ttttgaatat atagctaaac aaaatccaga agttatgttc
    18901 gttatagata gaggaatagc tactggtagt gatgtaaaag aaagctcaac tgcaaaatct
    18961 gttttgaaca atgatataat aaaatctatg gatgcatata agaatgataa tataatatat
    19021 ttagattcac caacatggta tgtaaatgat ggtggtctaa catctttaaa taaaatgata
    19081 gatgatgctt caaaagcggt aaattaataa tccgatataa tcaaaatgta ttttttgtaa
    19141 aatgattatc aaataaacta ggagttttta agaactccta gtttattttt tgcataataa
    19201 agatatagtt taaacaataa tattatttat aataagataa atctattcaa aatctaataa
    19261 aatatgaagt tatactagac aaaaagaata ac
//
LOCUS       DAELXX010000074        19198 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.074, whole genome shotgun sequence.
ACCESSION   DAELXX010000074 DAELXX010000000
VERSION     DAELXX010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19198
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.074"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            143..1141
                     /locus_tag="KPH29_002488"
     CDS             143..1141
                     /locus_tag="KPH29_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HBG1468074.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            1503..2030
                     /locus_tag="KPH29_002489"
     CDS             1503..2030
                     /locus_tag="KPH29_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HBG1468075.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            2057..2653
                     /locus_tag="KPH29_002490"
     CDS             2057..2653
                     /locus_tag="KPH29_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HBG1468076.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEVAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKDVIDTVSISLNAPNKEAYNRVTQPKFGEKSFDYMLGFARDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(2718..4301)
                     /gene="asnB"
                     /locus_tag="KPH29_002491"
     CDS             complement(2718..4301)
                     /gene="asnB"
                     /locus_tag="KPH29_002491"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBG1468077.1"
                     /translation="MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIGRYWKPEFHPDHSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEERFDESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNIYHKAECYGENLKTFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDQYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
     gene            complement(4867..5703)
                     /locus_tag="KPH29_002492"
     CDS             complement(4867..5703)
                     /locus_tag="KPH29_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG1468078.1"
                     /translation="MNYIIKKVLNSSVVLVHDAKYNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKKFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
     gene            complement(5727..7193)
                     /locus_tag="KPH29_002493"
     CDS             complement(5727..7193)
                     /locus_tag="KPH29_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBG1468079.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDADV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASALATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLATQQKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIAKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIENNSIDFK"
     gene            complement(7196..9058)
                     /locus_tag="KPH29_002494"
     CDS             complement(7196..9058)
                     /locus_tag="KPH29_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG1468080.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDTNDSTVKTEEKINLGTRFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLATMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAFLPFMVATGMHKALIPYSINTIGKLGYEALYMTSSLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAMLGVVFSSAISGTFLG
                     LFAVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNTV
                     ESSIGDVVEEELEEEKEVNILVTPVEGKVIDLSKVNDELFASKTLGDGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNNNGADYAYKVLNQSYGNVKKGSILFDVKGGI"
     gene            complement(9305..10168)
                     /locus_tag="KPH29_002495"
     CDS             complement(9305..10168)
                     /locus_tag="KPH29_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBG1468081.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFSNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            complement(10223..11155)
                     /locus_tag="KPH29_002496"
     CDS             complement(10223..11155)
                     /locus_tag="KPH29_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HBG1468082.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKLGEKLNVPVFKHKILKNPKDLPIW"
     gene            complement(11241..11792)
                     /locus_tag="KPH29_002497"
     CDS             complement(11241..11792)
                     /locus_tag="KPH29_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1468083.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            complement(12021..12512)
                     /locus_tag="KPH29_002498"
     CDS             complement(12021..12512)
                     /locus_tag="KPH29_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468084.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            complement(12540..13100)
                     /locus_tag="KPH29_002499"
     CDS             complement(12540..13100)
                     /locus_tag="KPH29_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBG1468085.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            complement(13252..14409)
                     /locus_tag="KPH29_002500"
     CDS             complement(13252..14409)
                     /locus_tag="KPH29_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG1468086.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRHFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDSILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            complement(14446..15387)
                     /locus_tag="KPH29_002501"
     CDS             complement(14446..15387)
                     /locus_tag="KPH29_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468087.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLKQDNKKNEEISNNKNL
                     NNKQDRNIQNKESNEEGSNDIKEKNNSDSNVSVNNKSEKQEDNTKENNKYTSKEKNSE
                     DIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNK
                     CKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLINTKSPKYI
                     GKITEYLDLSDKQIESFKNRLNDLKEEINNSETNNIN"
     gene            complement(15474..16166)
                     /locus_tag="KPH29_002502"
     CDS             complement(15474..16166)
                     /locus_tag="KPH29_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG1468088.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            complement(16276..17088)
                     /locus_tag="KPH29_002503"
     CDS             complement(16276..17088)
                     /locus_tag="KPH29_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBG1468089.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            complement(17139..18050)
                     /locus_tag="KPH29_002504"
     CDS             complement(17139..18050)
                     /locus_tag="KPH29_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG1468090.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKSIEVGKIELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            complement(18109..18840)
                     /locus_tag="KPH29_002505"
     CDS             complement(18109..18840)
                     /locus_tag="KPH29_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBG1468091.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFDKLNDVYKSLPVGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMILDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLV"
     gene            18929..19180
                     /locus_tag="KPH29_002506"
     CDS             18929..19180
                     /locus_tag="KPH29_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468092.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGTKFINKKR"
BASE COUNT         6462 a         3293 c         2083 g         7360 t
ORIGIN      
        1 caaaataaca cagggaaaac aatttcaaaa ctaaattttt aaccttaaaa attttgtata
       61 aaaaatataa ccttatatgc tacatattat ttctaaatga aaaattacca taataattta
      121 ataattacag gagggtattg atatgaaaat actagtattt ggagcacgcg attatgaaga
      181 accagtaata aaaaaatggt ctgaagaaca taaggatgtt caagtggata tttatcctga
      241 aaacatgact gaagaaaatg tagttaaagc taaagggtat gatggtatat ctatacaaca
      301 aactaactat atagataatc cttatattta tgaaacttta aaagatgctg gggttaaagt
      361 tatagcttca agaactgcag gggttgacat gatacatttt gatttagtta atgaaaatgg
      421 acttatcgtt acaaacgttc cttcttattc acctaatgca atagctgaat tagctgttac
      481 tcaagctatg aaccttttaa gaaagactcc tctagtaaag aaaaaagtct gtgaaggtga
      541 ttaccgttgg atagctgaac ttcttggaac agaagttaga tctattacag ttggtgttat
      601 aggtacagga aaaataggtg ctacttctgc aaaattattc aaaggcctag gagctaatgt
      661 aattgcattt gaccaatatc caaatagtga tttaaatgat atattaactt acaaagattc
      721 tttagaagac cttctaaaag aagctgacct tataacatta catactcctt tacttgaagg
      781 aacaaaacat atgataaata aagatactct agctataatg aaggatggag cttacatagt
      841 aaatactggc cgtggtggtt taattaatac aggggattta atagaagcac tagagtcagg
      901 aaaaattaga gctgctgccc ttgatacatt tgaaactgaa ggattgttct taaacaaaaa
      961 aatgaatcct ggagaattaa ctgacccaga aataaataaa cttctttcta tggaacaagt
     1021 tatattcact catcaccttg gtttcttcac tagtacagcg attgaaaata tagtttattc
     1081 tagtttaagt agtgctgtag aagttataaa aacaggaact gctactaata gagtaaatta
     1141 gtattttata aatatatttt ttcattaatt agctattatc taacaaaata gcctttaatt
     1201 tcccaaatat aagagagtca gctcagtata tattgagtag actctcttat attatttttt
     1261 atattaaact tgtactcctc ttatttatac caaattaaat ttatattaac ttactctaac
     1321 tactcctact ctatacattt tacctatttt tacttttcta attatataca tttgaatggg
     1381 tacaatctgt attttacaaa aaaaacacct aacaaactat gaaaattgta tgattaatat
     1441 taatatattt ataagaaaac ataacaatta tgttagctct ggatttttag ggagggagta
     1501 tattggaaaa aatgaataaa gttacttcta aaaaaactac ctatattgtt acttcagcat
     1561 tatttgcatc cataatatgc ctgacaatag catatatatt acatattcca gttggtggaa
     1621 acaatggata tgttcatata ggagatgctt ttatttatct agctgcaact attttgccaa
     1681 caaactatgc tatagctgct tctgcaattg gtgctggtct agctgattta tctacaggtg
     1741 ctgctatctg ggtaatacca actataatca taaaaccaat acttgtatta tttttcactt
     1801 ctaaaagtga caagataata aataagagaa atatagttgc ttcggttgta gcaggtatag
     1861 taggattagt tttatacatg tttgctgaag gtataattat tggaagtttt acaagtgcat
     1921 ttgtaatgag cttacttggc ttattacagc caataggaag ttttattgtt tttataatct
     1981 taggtatggc acttgataaa cttgatttta aaaaacgtta ttttaattaa gaatttaaat
     2041 ttatggaggg aaaattatga atatcttata tacaattgaa aatagtatct atgtaaatat
     2101 aactaataca tgtccatgta gttgtgtttt ttgtataaga aacgaaaaag atgaagttgc
     2161 aaatagtggt agtttatggt tggagcatga gccaagtgta gatgaagtaa aagaagcctt
     2221 caataaatac aatttagacg actatgatga aatcgtattt tgtggatatg gcgaaccatt
     2281 aatgagaata aatgaattga tagaggtagc aaagtttata aaagaaaaaa gtagcataaa
     2341 aataagaata aatacaaatg gtctaagtga tttaatacat aacaaaaaaa cagctattct
     2401 gttaaaagat gttatagata cagtatcaat aagtcttaat gccccaaata aagaagctta
     2461 taatagagtt actcaaccaa agtttggaga aaaatcattt gactatatgt taggttttgc
     2521 aagagattgc aaaaaatata taaaagaagt agctttttca gtagtagatg aaatatctcc
     2581 agaagaaata gaagaatcta aacaactggc aaaaaaactt gatataccat taagagttag
     2641 acataagaat tagcaagtaa aattataaaa cattaagaat cacctgaatg gtctttcagg
     2701 tgattcttcc tcttgtatta acattttaca aaatagattt cataccacac taggaatgtg
     2761 taaatcatcc aaatctttct ccaattatca gagtttccac taatgtagtc attaaaaatc
     2821 tcattaatct catctatgtt aaagaattta tctgctatct cactattgaa tttatctcgg
     2881 atatccttat tatattggtc atcagccatc catatacgaa ttggcacaat aaatcctaat
     2941 ttatttctaa aagcaatctc ttctggtaat gtttttgatg cagctgtacg aaaggcaatt
     3001 ttattctctt ttcctttcac tttatattct gatggtattc tggatgcaat atcaaatatt
     3061 cttgtatctg taagaggcat tctgacttcc aatccaactg cagtactcat tttatctata
     3121 ttaaaataaa tatctccttc caaccatatt tgaatatcta catttgacat gttagtaagt
     3181 gtatctagtt cttctgtttc ttcataaatt ccttttacca cttcaatagg aagcacatca
     3241 ggattgtatt tcttaagaat ttttcttttc tcaccctcat tcataatgtt tgtatttcca
     3301 atataaaatg tcttcaaatt ctctccataa cactctgcct tatggtatat attatatcca
     3361 ccaaaaaact catctgctcc ttctccagag taacagatat ttgtgttttc agctgctgcc
     3421 ttgcatccaa tagcaaaaac gatagcagat gcatctccag ttggttgctc catatggtac
     3481 attacataag gtactgacgc aaaatactct tcaggtgtaa ttatatatct cgaattttta
     3541 cacccaagaa tattagctgt ctgttgtgct aattttgatt catcaaaacg ctcttcctca
     3601 taaccacatg aacctgtttt ttctatatct gacattgcca atatataaga tgaatcaaca
     3661 cctcctgata agaataactc tgcagtttca ttctctgatt ttacctctgg cataattttc
     3721 tgaagtgttg tatgaatttc atccgtccat tcctcaatag acttgctgtg gtcaggatga
     3781 aattcaggtt tccaataacg accaatacat actgttttat tctgatagac aaggtaatgt
     3841 cctggcataa gcttctttac tccacggaag aatgtatttt cacctgctac atatgtgaaa
     3901 gacatataaa tctgtaacat gtcttcattt aactctttca caaatcctgg ttgctccata
     3961 atcttacgaa tagatgaacc atagagaagc ttcccatttt cagtctcata ataatagaaa
     4021 ggttttactc caaactggtc tcttaggcag aagagtttct tttccacagt atcccataat
     4081 gcaaaggcaa acattccatg catgtgattt gccatatcat atccccatgt cttatatgct
     4141 tccactaaaa ttgctcgttc acgctcatct ctacataatg aagaatcgat tccaagttct
     4201 tcacagagaa ctttccagtt tcgaatatgt ccattgtatt ctagaatttt tatcatatat
     4261 gtgtcttttc gtgttaaata cttttcttct aaatttatca tatgtgtatc ctttcgtatt
     4321 aaaacattta acttctaaat ttattaaatg tttatatata tttattcact tataatcact
     4381 gaaaaatcga cttttttcaa agtaaattct ttttgaatta gtactaatct gctctttatt
     4441 aatggcataa atatgattat caagtattat cagtgttata acattgaatt ttttattctt
     4501 cataaatatt gcccccttta tattaccaac taaatataat atatcttatg ttacttacat
     4561 attctttaag aaattctgaa gattttataa agttttttgt attataatta attaaaaata
     4621 aaattctagt tataaaacat attttccatt aaacaaaaaa atagttaaaa taaattcata
     4681 accacataaa cttattttaa ctatttgtat attttaatta taaatattaa cttaaatttt
     4741 taacaggata cactcataaa ataaacataa tgaacaacaa aataatatgt ctcagtttct
     4801 ttatatactt taccttcttt caattttcta ccatgtagac gtataagata atattagtaa
     4861 ttcactctat tgcttaactc gtgcgatatg gacaaccaag aaagctattt cctcatttgt
     4921 taactttttt ccatatttct tttctaaaaa atctctaact tttaatgcaa ttaacatttc
     4981 ttttggattt gaccttttca tttgattaaa taataaatca tcaccactgc ttaacatact
     5041 atctgaaaaa aatctttcca caaaaaattt tatatgtgtt ataaatctca tataatgcat
     5101 actcttttta tctaaggttc tattcaaaga aaaaacaaaa atattaatta tttctccaat
     5161 tactttggca tacttaatcg tatcatatga atttccatct gaagccatag catttgcaaa
     5221 atggaaagca atgttagctg cttcttcctc tggtaattga atgcccaatc tctcctctac
     5281 catataaatt gcaaccattc caaccttaaa ctcatttgga taataatttt ttatttccca
     5341 aaatactcga tttgtaattt ttattccttt acgcatcctt tcaattgcaa agttaaaatg
     5401 gtctgctaat attaagtata agtttttatt aaaagatgag ttcaataact tttctgcctc
     5461 tactattatt tcctgtgtta tctttaaaat gtcgacagga atatcatcca ttaattctaa
     5521 aaattttttt gattttgtat tttcaacagg gacaaacatt tgattatcat tggaaccaca
     5581 aatatattct cctgtttttt taccatatcc aattcctttc cctagcaaaa taaattcatt
     5641 atactttgca tcatgaacta aaacaacgct cgagttcaaa actttcttta ttatgtagtt
     5701 cattttattt gctcaccttc tattggtcat ttaaaatcta tactattatt ttcaattact
     5761 tttttatacc aataaaaact atctttctta tatcgattat atgtcccctt tccataatca
     5821 tcagcatcca cataaatata tccataacgt tttgacattt gtttcgttga ttcactgacc
     5881 aaatcaatac aaccccatga agtgtatccc caaagttcaa cgccatcttc gataatcgca
     5941 tctaacatac atttaaagtg ttcttttaaa taattaattc tatattggtc atgaactttt
     6001 ttatcttcag ttaaaatatc tttagctccc aatccatttt caactataaa taaaggcttt
     6061 ctgtatctat catataattc aatcaaagat atacgtagac ctattgaatc aatttgccaa
     6121 ccccattcac tcatttctaa ataaggattt tttacagcta ataacgtatt cccaggtgta
     6181 atatctaatc cagttgtatc agctgcctca caagaagaca tatagtaaga gaatgaaata
     6241 aaatctactg gatacttttt cataatttca tcatcatgtt cttcttttgc tatttgaatg
     6301 ttattatttt taaaatacga aagtaaatat actggatact caccaaaaac ctgcgtatca
     6361 ctatatgcat aaatacttct cattttttgt tgagttgcca aaacatcttc tggtctacat
     6421 gtatatggat aatacgttaa ttttgttaac atacacccta cttttgaatt tggaataatc
     6481 tcatggcaaa tttttgttgc aagggcacta gctacaaatt gatgatgcat agcttgaaaa
     6541 ataacttcct caaagttttt atccttaaaa cggtctcgaa ctaaaccacc agtagtgtac
     6601 ggatgacgaa tcatagaatc aacttcatta aatgtaagcc aatatttcac tttgttttta
     6661 tatctttcac agatagtttc tacataccta acaaacatat tgataacttg tcgtgaatac
     6721 catccatcat agtttaaaac gatatttaat ggtggttcat aatgtgacat agtaacaagt
     6781 ggttcaattc cataacttat caactcatca aatacatcat cataaaactg taaaccttct
     6841 tcatttggct ctttatcatc tccatttgga aaaatacgag accaagcaat actcattcga
     6901 tatgtcttaa atcccatttc tgccattaga gcaatatctt ctttatagtg atgataaaaa
     6961 tcactaccat gacgttttgg ataatttact tcatcttcag gatgtgctaa tccttcttct
     7021 atatcttttg ttgtgatttc attattagat ttatagtctt tcacatctgc atcaaagtgt
     7081 gcctttgcta catctgatac agaccaccct ttaccaccta aattccaacc accttcaaat
     7141 tgattagcag ctgtagctcc tccccaaaga aatccttctg gaaactttct catatttata
     7201 ttcctccctt tacatcaaac aagatacttc ctttttttac attgccataa gactggttta
     7261 acactttata agcataatct gctccattat tgtttacaat cataacagtt gaatctaaat
     7321 tagcatgtga gattttgtct aaatcaaatt gaattaataa atctccttct tttacattat
     7381 cgcctgtctt aacaaatgtt ttaaatccat ctccattcat acttacagta tttattccaa
     7441 tatgaatcaa aatctcagcc ccattttttg ttcttaaacc aattgcatgt tttgtttcaa
     7501 ataacatcac aacttctgaa tcacaaggtg cgtataaatt tccattggtc ggtataattg
     7561 caactccatc tcccaaagtc ttacttgcaa atagttcatc attaacctta cttaaatcta
     7621 ttacttttcc ttctactgga gttactaaga tatttacttc tttttcttct tctagctctt
     7681 cttcaacaac atctccaatt gatgactcta ctgtatttgt atcttccttc caaataatca
     7741 gtgtaataat aaacgagcct aatattgcaa taacaaaacc aatactcgca aaaatcaaat
     7801 tgtttccatt gtttgggtca ataaacatac tcatactggc aagccctggt ccaacaatta
     7861 caaatccttt tactgcaaat aatcctaaaa atgtaccaga aattgcactt gagaatacaa
     7921 cacctaacat tgctctttta tgttgtaaag taataccata taaagctggc tctgtaattc
     7981 ccatcaaggc agaaattcct gcagataatg ctgtttgttt taatgtttca tcttttgttc
     8041 gcaaggctac tgcaaaacaa gcaccacttt cagaaatatt gtgggctaat gaagatgtca
     8101 tatataaagc ttcataaccc aactttccaa ttgtattaat tgaataaggt attagtgctt
     8161 tgtgcattcc tgttgcaacc ataaatggaa gaaaagcagc aagtaatcct acaactaaga
     8221 atcccatttt gttatataag aataaaataa tggttgtaaa aattgtacca acgttatatc
     8281 ctaaaggacc taaaatagtt agagtaattg gaacagtaat tgccaaagac atcataggaa
     8341 caaagaaagt tcgaattggt tttggtgaat acttgttaaa aattttctcc attgttgcca
     8401 aaaaagacac acataagata gccgggaaaa cttgtgcatt ataggcaatg tttggaattg
     8461 taaatccaaa tagttcgaca ccttcactta aagcagttgt tgttgctgga agcaataagt
     8521 atcctacagc ggcaatagct actaaacgat tacatttcaa tacattggca gtagttacag
     8581 ctaccataag aggtaggaaa tagaatgttg catctgaaat agcaactaat aatgtatata
     8641 tagtagaatc tgattttaaa atattaaaca ttgctaataa catcaacagt gattttaaaa
     8701 taccagctcc tgcaatagca aaaatcagtg gttgaaaaat attaataata aattctataa
     8761 atcttgttcc taagtttatt ttttcttcag tttttacagt actatcattt gtatctaaag
     8821 ggcaaacttt taaaatttca tcatacacat caacaacact atttccaatg acaacttgaa
     8881 attgtgcccc attaactata ctcataacac cttgtacttt ttttaaagca tcctgattga
     8941 ctttacttct atcaatcaac ttaaagcgta gtctagttga acagtgttct aaagctaaaa
     9001 tattttttga accaccaact tgttcaataa tgtcaatggc agttttttta taattcatct
     9061 tgtaatctcc ttctccataa ttttttatga atggccattc aagatgcaaa aaaggcagaa
     9121 cctatataaa caccttgaaa aatcaaagca atatattcga tcctgcctgc attaccagta
     9181 acatgtcttc tatttatact attttgtatt attatattaa catttgccta aaagaattgt
     9241 caatactttt ttaaattttc tgatactaac taatagcacc tttataattc attttttatg
     9301 tcaattaaag taacagtagc cacccaccaa ttagtatcaa aattgaacct ggtatttttt
     9361 cttttatatt ttctttgaat ataaaatgag caagtattaa agttattatc atagatactt
     9421 gagaaaatgg ctctgctata ctaagttcta gattatttaa agcaattaat aatgctaaat
     9481 aagaatatcc attaatagca ccacttaaaa ttgaaagccc ctttttatta ctaaaaatct
     9541 cacaaattat tttttgtttc tttttaaatg ctaaaaagat aaataaattt aaacctacga
     9601 aaaaatacag tatagttgaa tataatatag gagatacttg tacaaaaaaa gccttatcca
     9661 atactcttcc tactgactgc aaaaatatgt ataaaaacat taatctacat ggtaaattgt
     9721 taaaaattac agagacattc tttaaaaaag agcttacttt ttttaatata aacactccac
     9781 caatcataat tatgattcct gcaacttttg ctaaacttaa accttcacct aaaaatataa
     9841 ctgataaaag cagtaaaaac aatgaattta aactatttat tggagtaaca agtgaaactt
     9901 ccccagttgc taaagacgtt acatacgcta ctgaaccaat tgaatagact aaactactta
     9961 tataacatgg tattaaaaac gaccaatcac taactctatc cattatgcta aatggaagta
    10021 aacatactcc ccctataaag aaaaatagaa atgatgaagc tatattttta taaatatctc
    10081 cttcttcatc acctaataat cttacaacta ctctttcaaa agctaatagc actactctaa
    10141 ttaataatgc aaaataagat aatatcattt ttgacctcgc ttaaaaatta atccgactta
    10201 ttgttatatt aactgtataa gactaccata taggtaagtc tttgggattt tttaatattt
    10261 tatgtttaaa tacaggtaca ttaagttttt cacctagttt acggcattgc tctttatagg
    10321 taccttttaa tttatcatga aaaactatac aatcaattcc accttcaata ctatttatac
    10381 acttttctag tttctcaaat cccgattcat catcatacat tccacttaca atccattgat
    10441 ttggcaatac atgtccaatt gatatgttat attctttatt ttttataata tctgagaaag
    10501 atttctcaaa atgtacccct ccaacacata ataaagtctt caaaggttca tcacaatcaa
    10561 atactctaat taatgatttt gccaatacct gtattgctat actattatta aaactatcac
    10621 tactacttcc tatctcaata tccaacatag gtacagaata ttctgtaatt aatttagatt
    10681 cttcaccata tgttgttcca gaccagtgag ttgcttctgt taatgtagta aattcatcaa
    10741 gtagattttc attttttata tcctcaatag cattgattag atttttaaag tatcttggat
    10801 ttgaatttcc aaattctcct gttggaacat ctccaattgt atgagctgtt aggatatgtt
    10861 ctggagcatt ttctccctca tgccaattta caactcctgc aaaatcataa tctctgaaat
    10921 gtctatttaa aattggaaga taatatttat aattatgact tattacatct tttgttttta
    10981 caaaatgaaa taagttattg tttttatcct taaattctaa tacaaaatta tcatcaataa
    11041 caatatcagt ttgatttaaa tccattattt cttcacaaca ctttatcaca ttactggcta
    11101 ctgggtcttt ctttaaatca ctacaaaaga aaaaaactgc ttttttatta ttcatccata
    11161 aatcacactc ctataactta aatataatat atcttcatac ctaaaatcat ctaaatatca
    11221 ctctaattcc attaaattat ttatatataa tatactacca tactcatttt agctatacct
    11281 ttgcctgagt ttctatatat atgtgataca tcagctttaa atcttataga atttccacta
    11341 gatacaatgt aatcttctcc atttatttta atggttactt cgccactaaa aacagttata
    11401 aactcttgtg ttcctggaag ttcagcattt gaatctaaat ttccaccttc ttctatttca
    11461 atcataaata tctcaaatct tcttttggag tcaaatggaa atattggata acttatatac
    11521 tttccattat cttcaataaa atggtctata tcactttttt gaatcaattc tatgtcactt
    11581 tcctgcctct ccatcaatga tgtaaatgag atttttagtc cattagttat tttcttcaaa
    11641 gtagatatag taggatttac ctcacacttt tcaatttgag atagcatact cttacttaca
    11701 ccactaagtt gtgcaatctt gtctaaacta agatgttttt ctttccttat cttttttaaa
    11761 ttttcagcga ttatttcatt taaactttgc ataaataccc cctaatacat actaatataa
    11821 gataaattat tttgattatt aaataaactt agtaatttaa tagaaacttt ttatccatta
    11881 tactgtacat tatgtttaat atacattact ttttttagaa tgtaaatagt tttctgaaat
    11941 tttgtgaaag tgaacaaata aaaaactagt aacctatatt tttacaaggc tactagcttt
    12001 tattataata atatttcatt ttaactataa aacttagatg cacctataag ctgtctctta
    12061 gaaacaatct tagctaggtt tgctaatgaa gaaattttta tatctttaaa agaaactaaa
    12121 aatacaaact taaaatctgt aaattcttga tttatattgt atatatcaaa ataacatctc
    12181 aaattatcaa ataagtaatt atcttgtaag tctgcttcta tctcatcagt agcatctttt
    12241 aaatacgctc ttacatcttc aaataaaaca ctgaaataag actcatctat ttcttcacct
    12301 aactcttcca atatttcttc tatatctaaa tcatccatat caaaagaatt tcctgtcaac
    12361 tggcaatcaa ataccaatat attttcttga tttatagtat ttgatatatc ttgtacatat
    12421 tcgaagtctg gacgtaattc taaaatgtct tttatttcac tgctctttac atggattttt
    12481 ccttttctat tttcaatttt taaatctatc atatgctccc cctttcaatc tacaaatctt
    12541 taaatacctt gttttataat cttttcaata tctatccaat tactaactct aactatattt
    12601 tgatgtttaa catctttatt atagttagta tccagaagta ttactttaac accatcatca
    12661 gctagttgca ctgagtttaa aggattatct tctataaata tgtcacaact cagttctctt
    12721 gccttttcaa ctttataatc acttcctaat aagtaaacat ctatatctga aaatccttgt
    12781 ctcgatagcc aatttttagt tacatcagtt aaacactcat gtctagccgt tacaaaagat
    12841 aaattatgtc ctttagataa tgtgctaatt acctctgtag caccctctac aatctttgct
    12901 tcatcatatg agtgtatata cttattatta aactccgcat acacatctct tattcccgaa
    12961 ccatacaatt cttcccaatt atgtgttata cactctgctt cagttatatt tttattaaag
    13021 atatcattta ggtatggaag aaaatgataa gggcttgtta ttgttccatc tatatcaata
    13081 catatattca atttgttcat ttttacctcc atgtaacatt tattgatttg attaatcaaa
    13141 tattatagca cagctttaat tataaatcaa aactctggat atgactatac ctatataata
    13201 ccaaaaagct tgcatattta agcaagcttt tcgtaaattt tacattttta actaatatat
    13261 aagtttttct aaggttggtt ccactccaaa ttgtgatttc agttctttat attcttttaa
    13321 tgctttgtca ttattcgttt tagtttttat cgctcttatc attaaatttt ttggtgtatc
    13381 aagtggagat atgtattcta ctacagaagt atcatatcca ttgccctcca ataacaatgt
    13441 tcttaaacca tcagttatca aatctgcaaa tctagcttta aatacaccat gttttaatat
    13501 tggttccata gcttcatatc tatattgccc taataactcc ttatgacaac atggtactac
    13561 tactatagct tcagattttg ctctaattcc tagacctatt gccatatctg tagctgtatc
    13621 acaagcatgt agacttatta caaggtcaac atccctatta ggtgtataat tagtcaaatc
    13681 ctcttttata aatgacatat ttttataacc caagttttta gccatatttt ttgatgcttc
    13741 tataactaca tctgaataat ctattcctat aaaataacag tttttcttca atacttcttt
    13801 tatataaaaa tttaagacaa agcttaagta agattttcca caagcacaat ccaagatagt
    13861 tatacagtct ttatccttta tttcttttaa aatactatct atcaattcaa caaaatggtc
    13921 tatctgattg tattttctta ttttatcatt ttttatctta ccatcttttg taagtactcc
    13981 aatttctttt aaaagtgcat ttgcttgtcc tacttttaca taataatctc tgttttttat
    14041 ttgagatgcc gtaaattcat ctattttagg tacttcttct ttattatcta aatattttac
    14101 atttaccttc ttattgtctc cttcaattac aacaatagtt cctcgctcaa catatttaaa
    14161 tactaatcca tcataatttt ctgcctcttt agaaatattc ttaagcaact cattaaacgt
    14221 atataaatct gattttcctt taaaattaaa tttatattta ttatcttcct ctattgcaat
    14281 tcctttaaac tcttttcttc cagatttaaa aatcacctct aactcaataa aaaaatgcct
    14341 attatcaagg tttctttgag taatccccat aaaaaacata tttagttttc ctatactctg
    14401 tttattcatc ataatctccc ttttaactta tatctttaaa tcttattaat taatattatt
    14461 tgtttctgaa ttatttattt cttcttttaa atcgttcaat ctatttttaa aactttctat
    14521 ctgcttatct gataaatcaa gatattctgt tatcttccct atatatttag ggcttttagt
    14581 atttattaat ttcaaagcac tttccataga tttttctcta agctcatatg ctttctttac
    14641 gtcatctcta gcattactca atgtttctgt atacttctcc ttagaaagtt ctggaatctg
    14701 catattttca tattctttta caacttcttt acacttgttt ctggcatttg taacatcttt
    14761 ttttatacta gataatttag ttatattttt tgctatatcc ttagtgtaag gtatacattc
    14821 tctcattgga actaaagctg ctctttctat attataaatt tctttcttaa aaattcctat
    14881 actttccata tctatttttt ttatatcttc actatttttt tctttacttg tatatttatt
    14941 attttctttt gtattatctt cttgtttttc tgatttatta ttcactgaaa cattactatc
    15001 agaattattc ttttctttta tatcattact tccttcttca ttactttctt tattttgtat
    15061 atttctatct tgtttgttgt ttaaattttt attatttgat atttcttcat tctttttatt
    15121 atcctgtttt aaatcggtac tttctttttc cttaaaattt gatacatact tttcatcata
    15181 tgtctcatca gaaatataat tgtgataata catagactct tttattttat taagtccact
    15241 catataaata ttacttatca ttattgttgt agctaatacc acataaatac tactgtagat
    15301 acaagacctc ttaaaattct tgtaatttct gaatatcact actaaaggaa taagatataa
    15361 aaatataaaa actaatatcc acctcataag tcgtgtctcc tttctcaaaa ccgaaatccc
    15421 tcaaatattt atttttaatc atgtatctta tagtatttac caatttatgt tttttaatcg
    15481 tgaactttta ttaaattaaa ttctgtatag tcacaagatg ctaatgtata ttctactcca
    15541 tttcgaatcc ccttcaatcc cattttaaaa gattctttac catgcaagtg accataaatc
    15601 accttttcaa cattatattc ttcaaataac tttgtaaata aagattcttc caatttatca
    15661 ttcgtaggag gataatgagt aatcacaatt atttttgagt gaccactttt acgagcagat
    15721 tctaatgata gtctaagtct atgctcttct cttttataaa ctttttcatc agtctcatca
    15781 aatttaacat cattaggaca tatccagccc ctacctccac aaattgcata atctttatat
    15841 tcataaaaat ttgtctgaat aaaacgcatg tcttcatata gcttatttaa acttgtaacg
    15901 gttgtccacc agtaatcatg atttcctttt ataagtattt tctgacctgg aagtttgctt
    15961 attatatcta aatctttttt agcttccgat aaattaattc cccaagatgt atctcctagt
    16021 actaagacca tatcttcttc cttaacaact tctttccaat tatctataat tttcttttca
    16081 tgaccatccc aattacttcc aaagatattc attggcttat tgactgatgt tgaaaaatgt
    16141 aaatcaccta tagcatacaa actcataata tcattccttt cttaattaca ctaaataggc
    16201 tgtctctttt tagaaattat tttccattaa gaggcagccc cattaatatt tagcaaattt
    16261 tctacaagta gttttttact gaactacttt atcatctacc ttagtatcct tatattgctc
    16321 tagtaatata tctatttctt gttttaattc caaaatttca gttatatttt tatctgtttc
    16381 agttctatca agttttcttt ggtactgcat agctttatgt gtatcaccca agtcatataa
    16441 ctcttgtatt ctatttagag tatctctaat agctatagac ttcatttggt acatagcctg
    16501 agctttcatt atgtcctctc ttatttcact catttcttgg tctaaaagaa aggcagcatc
    16561 agatgctctt ctaagccttt cagcaagagt ctcaggtatg taatggtcat atttatattt
    16621 taatgaatgt tctattgtag cccagaaatt cattgcaaga gttcttattt gtatttcaca
    16681 tataatttcc tttgaacccg caatggagtt tatagggtat tttattataa catgatagct
    16741 tctataacca ctatctttgt aatttgtaat ataatctttt tctcctatta tagtcatatc
    16801 atttcttact tttatcaaat caactatagc atatatatcc tcaacaaact ggcacattat
    16861 tcttatacca gcaatatcat caatttcagt ttctatatct tttgcattta tcctttttaa
    16921 cttagatatt atggaagata ttttttttgt tctgcctgtg acaaactcta tgggagaata
    16981 ttcacctttt gctaaatatt cttttcttat atttttaaat tttattttca attcctctac
    17041 tgcatgttca taaggtgcta gtatctcatt ccatttttcg tattccatat tgctcaccct
    17101 ttatttgttt tttatgacct tctttatttt atcataattt attttgtttt tgtatagtaa
    17161 agcaattctc cattttccaa tatataccct atttcttcta aatcaactaa gtaacttatg
    17221 acagatacta acgaagattt aaaaaaatga tactctttat aattattgct taaattattg
    17281 ttatttataa tgttttttaa taaaatttct aaagatttcg gttctacaag caactctctt
    17341 atttgatata aatatttttt taaagcattc aaatgttttt ctattaattt ataagaatct
    17401 tctttcgata taacttgctt gccatgccct aaaactaagt actcaaaatc tatatttttt
    17461 aatttttcta atgaatttat ttgttcttgt atatcatata gaaacaagaa atcgtatttt
    17521 gatagaattt catctcctat caataaatcg ccaacaaaaa tcacattatc cttcgtcaaa
    17581 acagctatac taccaggtgt atgaccctga aactcaatta tatcaaacat ttcattgttt
    17641 aattcaattt ttccaacttc tatagattta tctatcttta tatcatccaa cattttattt
    17701 tttaagatat tatccataaa tacattgctc tttcccccaa caatatactt tgaaaacaat
    17761 tctggatttt ctatatataa tttgtcgtat tcagatgaca ttatacacac atctttataa
    17821 tattctttaa attgattaca agctccatag tggtcatcat gaccatgagt atttataata
    17881 tatctaacct gtatattatt ttcatctaat actttattta ctctcctagg tcttgaacca
    17941 cataaccctg ggtctatcat aagtgctgtg ttatcttcaa atacatatat tcctgtattc
    18001 gtgccacctt ttataaaaaa ggtatttcct tttacttttg taagattcac cttcctcacc
    18061 tcattttgac aatatttatg atacaattta ttacttacta atataaaact atacaagaac
    18121 aattcttttt attctatcta tagttctttt tcttattttg tattctttag atatccttat
    18181 tttcacatta tatgctaaat ttctataaag taatgactct ataaaatatt caactatacc
    18241 tctatcccta aagtctatat ctatatttcc acttgaataa atatttgagt ctaatatcat
    18301 aagctcatca aaaaagctta atctcttaga tttacttaaa taatctttat ttggaataaa
    18361 tctatcacaa taatctatcc tgtaatttac tacttcatca tttatttcaa atccatattg
    18421 ctttttcatt aactctctat agttattatt tttctcaata acattatcta aatttttatt
    18481 ataatcctct tttttccaat gtccaaacat cctacaattt aatggtctaa cttcatatat
    18541 tagacatcta ttattagtat ctttaaatgg acatgggttc tttcttgtat actcctcaaa
    18601 ataataatct aatattttac ttatacagct tttttttaaa tcaactctat cttcaagata
    18661 acaaaatata ttcaaaaact caattaaatt tatacctact gactccatac aacaatttcc
    18721 acatccagaa caatttccta ctggcaagga cttatataca tcatttagtt tgtcaaagag
    18781 cttattttta atggaataat ctatacaatt taaaatatct ttatttttta tactagtcat
    18841 tattattact ccttgttata attattaagt taatacaaac aattattata acatattttt
    18901 taaatttaat cgcatatgga ggtattttat gaatagatat acaaaaataa ttaatatgat
    18961 gggaagttat tacacaaaag actttgaaaa agaaaaaaag aatgtaataa aagttagaga
    19021 agttaaagaa gatacagtta gaaaattttt cttgcaaggt gactgtgaag ttcttgtagt
    19081 atttgaagac actgggaaag aaatacttat tgatgacttt tctcctgaag aagatatcaa
    19141 aaaatattta ggaacaaaat ttataaataa aaaaagatag ttatcaaaaa taattcta
//
LOCUS       DAELXX010000075        19040 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.075, whole genome shotgun sequence.
ACCESSION   DAELXX010000075 DAELXX010000000
VERSION     DAELXX010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19040
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.075"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..385
                     /locus_tag="KPH29_002507"
     CDS             <1..385
                     /locus_tag="KPH29_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468093.1"
                     /translation="SIIPYRILISMAIITTLWTILDLIVTIITLKKLDIKLNLLDDII
                     TDLNDINVKLDKFDRGEIQALFKTINNDGLEVREKINKINSKLDRIESNIILQKRVIK
                     AFPHIKHKKQQEQLEHFKKIINEKR"
     gene            677..1996
                     /locus_tag="KPH29_002508"
     CDS             677..1996
                     /locus_tag="KPH29_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG1468094.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMSIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(2071..2499)
                     /locus_tag="KPH29_002509"
     CDS             complement(2071..2499)
                     /locus_tag="KPH29_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468095.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(2590..3966)
                     /gene="hydG"
                     /locus_tag="KPH29_002510"
     CDS             complement(2590..3966)
                     /gene="hydG"
                     /locus_tag="KPH29_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HBG1468096.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIKEEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(3967..5052)
                     /gene="hydE"
                     /locus_tag="KPH29_002511"
     CDS             complement(3967..5052)
                     /gene="hydE"
                     /locus_tag="KPH29_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HBG1468097.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSPDEILE
                     CCDIGYKIGYRTFVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNKDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(5088..6296)
                     /gene="hydF"
                     /locus_tag="KPH29_002512"
     CDS             complement(5088..6296)
                     /gene="hydF"
                     /locus_tag="KPH29_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HBG1468098.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLVVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDNGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVNKDTPKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGILIAYVKGI
                     LERSLELFNY"
     gene            complement(6311..6562)
                     /locus_tag="KPH29_002513"
     CDS             complement(6311..6562)
                     /locus_tag="KPH29_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HBG1468099.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
     gene            complement(6943..7983)
                     /locus_tag="KPH29_002514"
     CDS             complement(6943..7983)
                     /locus_tag="KPH29_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBG1468100.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLEIKKDFENLGLDTI
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
     gene            8453..9613
                     /locus_tag="KPH29_002515"
     CDS             8453..9613
                     /locus_tag="KPH29_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBG1468101.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALVVTQILHMLA"
     gene            9956..10117
                     /locus_tag="KPH29_002516"
     CDS             9956..10117
                     /locus_tag="KPH29_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468102.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            10310..10939
                     /locus_tag="KPH29_002517"
     CDS             10310..10939
                     /locus_tag="KPH29_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBG1468103.1"
                     /translation="MKIVTYNIHKGMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQ
                     FLALKAVLNMDGVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            11178..12437
                     /locus_tag="KPH29_002518"
     CDS             11178..12437
                     /locus_tag="KPH29_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBG1468104.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLTYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            12696..13691
                     /locus_tag="KPH29_002519"
     CDS             12696..13691
                     /locus_tag="KPH29_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HBG1468105.1"
                     /translation="MYEEVTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRRNVLIK
                     VIKHNNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGI
                     GLDELILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDF
                     GITKANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNS
                     EEKMLQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKA
                     YIEVEDEKEEEQKQDKVIQVKKEYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            13937..15004
                     /locus_tag="KPH29_002520"
     CDS             13937..15004
                     /locus_tag="KPH29_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBG1468106.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHTGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFVEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSEDVET
                     IFSINHNFIEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKSKYSKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
     gene            15541..17451
                     /locus_tag="KPH29_002521"
     CDS             15541..17451
                     /locus_tag="KPH29_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBG1468107.1"
                     /translation="MNSNTFERLQLNEVKDLIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNAGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILSYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFISVNIKRLQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(17514..18137)
                     /gene="ytaF"
                     /locus_tag="KPH29_002522"
     CDS             complement(17514..18137)
                     /gene="ytaF"
                     /locus_tag="KPH29_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HBG1468108.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLELNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            18322..18966
                     /locus_tag="KPH29_002523"
     CDS             18322..18966
                     /locus_tag="KPH29_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1468109.1"
                     /translation="MEQHSKVGNFGDLLKVLLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQRPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
BASE COUNT         7311 a         2280 c         2892 g         6557 t
ORIGIN      
        1 aagtataatt ccatatagaa tattaatttc aatggctata attacaacac tttggactat
       61 acttgatttg attgttacta ttataacact taaaaaatta gatataaagt taaacttact
      121 agacgatata attacagacc ttaatgatat aaatgtgaaa ttggataaat ttgatagagg
      181 agaaatacaa gctttattta aaacaataaa taacgacgga ttagaagtta gagaaaaaat
      241 aaacaaaata aatagtaagt tggaccgtat agagtctaat attattttac aaaaaagagt
      301 tataaaagca tttccacata taaaacataa aaaacaacaa gagcaactag aacattttaa
      361 aaaaattatt aatgagaaaa ggtaattata caatttaatg aagacgaata gaataatggt
      421 atttataaga ttattttcag aggactctat aaaaatagaa tagagtcctt tattttttga
      481 aaaataatgt ttatttgata aaaataaaat atattgtttc taaataacaa atattttgaa
      541 aattataaaa acatatattg acaatttgtg tcaaaaatac taacattaag tatataaaga
      601 attttatatg agaatatctt tataaatcgc aataatttta gaacataaat ttatttaaag
      661 aggggatgat ttgccaatga gcgagataac tagtcaaatg ataagtacag aagaaaaaaa
      721 atatgttgct aaaactcaaa agatacctta ttatccagta gcatttaaat caggtgatgg
      781 tgcaatgcta tatgattatg aaggtaatga atatgtagat tttttagcaa gtgcaggctc
      841 tgcaaatgta ggtcatggta ataaggaaat ctcacaagca gttaaagaac aaatggatga
      901 tataacacaa tatacacttg catatttcca tagtgatcct cctgtaaaat tagctgagaa
      961 gcttgtggaa atagcccctg gagataatga taaaaaagta ctatacagtg caactggttc
     1021 tgcttgtata gatgctgcta taaaattagc aagagggtat actggaagaa caaagataat
     1081 atctatgtgt gaatcttatc atggaagtac ttatggagct atatctatat ctgctttaag
     1141 tacaaatatg agaagaaaaa tgggtccatt gttaccagaa gtttatcact tccattatcc
     1201 tgataaaaat agaacagcta aagaatgttt agatgagata gaatatgctt ttgcacacta
     1261 tctaccagca gaagaggtag ctgcaatatt tatagagcca attgcaggag atgcaggaat
     1321 aatagtgcct ccagtagagt gggtacaagg tttaagtaaa atatgtaaag aaaatggaat
     1381 attacttgta agtgatgaaa tacaacaagg tatgggaaga acaggtaagt ggtttggtat
     1441 agaaaatttt ggtgttgaag cagaccttat agttcttgga aaatctgttg gaggtggatt
     1501 acctttagga gcagttgttg gaagaacaga aataatgcaa agtcttgatg caccagcaca
     1561 tttatttact ttagctggaa atacaacagt gtgtgtcgct gctttaaaat ctatagagat
     1621 aattgaaaaa gaaaacttac ttcaaaagag tatagaaatg ggagattata ttaaagcagg
     1681 atttgaaaaa ctaaaagaaa aatacgatat aattggagaa ataagaggaa tagggttatc
     1741 tataggagtt gatatagtta aaggaaaagg ttctaatgaa aaacatccag atgctacagc
     1801 taagatatgt tatagatgta tacaaactgg attaataatg atattcttag gacaatctac
     1861 tttaagagta caaccacctc tagtaataac taaggagcaa gtagataaag caatgagcat
     1921 catagattct gcaatagatg attatttaaa tggtagaatt ggtgatgaag tatatgaagt
     1981 aactcaaggt tggtaaaata aatattaaat gaaaaaaaga gttatctcaa ttttagaaag
     2041 taatttctga aatgagataa ctcttttaag ttacccacaa ataagaaaat tgtttgattc
     2101 attaaagact atatttcgga tgtcgttttt gtgagtatca gagacagtat tacgagaata
     2161 attaccttta ttcgtactat ctgttaattt agttataatt aagatagcta aaagaataag
     2221 aattactgtg tataaagcaa taacatatgg ttttctttta tatttgtatt tgttattgaa
     2281 aaaagaatgg ccataattat ttttatttac tttagatgta tttgtattct ttttcttgtt
     2341 acctttcttt ttatcatcct tttttctatt tttagggtaa ggaatgactt tgtgtgtatt
     2401 atcaagtttg atatttttat ttttttgaat attgttttcc aggctagcct ttttttctcc
     2461 aaattgaata gttttattgg tattgttgtt atctttcaat gttaatctcc ctcccttaaa
     2521 ctttcccttt agataatatt actactaacc tatggaatat acaataaata atatataagt
     2581 aataaaattt tataggtata aatctctttc tccacgctct aaggcagtca tattgttaga
     2641 tactatgttt cttatatctt ctctttctat cttacctatt tcttctgcga taagtttttt
     2701 acccataatt ttaagttctt catctccata atcgattaaa aattcattta atgtagtaag
     2761 agcatttggc gtacatacat tatggatttg tcgactttta gctaagctca tgaatctatc
     2821 gccagttcta cctttacgat aacaagctgt acaataacta ggtatatatc catcagtaat
     2881 cagttcttta agcacctcaa cagggcttct atggtctcca acttcgaatt gttctgtatt
     2941 gtcaccatct tcatatgctt tatatcctcc aacacctgtt agagaacctg cactaatttg
     3001 tgatacacca tattttagta actcatttct catttcagca gtttctctag tggacattat
     3061 gattccagta aaaggaacag ccaatctagt tatagctact atttttttga acatatcgtc
     3121 agaaactaag tgaggaaaat cttctaagct cataccttca gcttttttca atctagggaa
     3181 agatatagta tggaaaccta caccaaattt ttcctctaag tgttcgttgt gcatcataag
     3241 tcctagaact tcaaatttag ggtcagataa accaaataaa actcctgcac caacatcatc
     3301 aacaccagct tccatagctc tatcaaaagc tgttaaatgg taataatagt cattttttat
     3361 agattgacca tgcattttta taaatgttgg tttgtgataa gtttcttgga ataatatata
     3421 agttcctatt cctttttctt ttagtaattt atactcatct acacttgtag ctgctatgtt
     3481 aacgtttacc cttcttatat ttccgttttc attatatgtt gaataaattg catctaagca
     3541 atctaagatg taatttatat cacaattttt agggtcttca ccagcttcta gagcaagtct
     3601 tttatgaccc attttttcta atattttaac ctcttccttt atctcttcca tggtaagttt
     3661 ttttctttta aatttgttgc atttactaaa accgcaataa acacattcat ttacacagta
     3721 gtttgataca taaagagggg caaataaaac cactctatta ccatatattt cattttttat
     3781 ttgcccagct atactaaata atctatctag ttgtttttta tcttctactt caagaagtgt
     3841 agctatatct ttataactaa gtttttccct tctatcagcc ttatcaagta ccttttcaat
     3901 atcatcagaa gttgaatttt ttgcatcttc taataaagaa ttaataagtt catggtttat
     3961 gaacatttac aaacgcctcc attcaatatt atcaccgcga gaatagtcaa cttcaagtcc
     4021 taatgacttg atgttttctt ctaaactttg gtgatattca caagcttctt gaccagtaaa
     4081 ggctttgtta tcgtaaagag aatattgttt tctaaattct tgaggagaaa ggtttggcat
     4141 aattacattg caaccagcta aaagacctgc atttctacca ctagggttta tagatgccaa
     4201 ggctgttgta gcgggtaata aaacttttgg aagtagaagt cttgtaattg aaagcataag
     4261 taatgtcttc tctaaatctc catgtttata atccttaaaa attgtatcat ggtgtggaat
     4321 aaaaggtcct attccaacca tatgaggatt taactctttt aggtataata aatcacgaac
     4381 aaggtctttg ttgttctgaa atggtgattc aaccataaat ccagcaccta cctgataacc
     4441 aatttcttta aggtttttta ggcattcttt tctagttcta agctcaatgt ttggcggatg
     4501 taatttttta taatgactgt ctgtagcagt ttcatgtctt aaaagatatc tatctgcacc
     4561 acataaaaag tattttttat acgaatcata actcttttct cctagtgaca gagttatggc
     4621 acaatcctca tatttatttt ttatacttga aacaatttca catattttat catcagtaaa
     4681 atatgcatct tcaccacctt gcaaaacgaa tgttctgtat cctattttat aacctatatc
     4741 acaacattca agtatttcat caggtgaaag tctgtatctt atgatatttt tattagaaga
     4801 ttgtattcca caatagtagc aattattttt acaatagttt gtaagctcta taagtcctct
     4861 caagtagact ctatttccat aatgtttatt tctcatgtcg ctagcttgtt tataaagata
     4921 gtttcttaaa ttttgttcga agttatattc ctcattgtcg atattttcta ttaaatatag
     4981 tagttcatca aaacttaatg aattaatgct gtaaagttta tctataaagt atttaatctt
     5041 atttttagtc atattcaaat atgtaattat taagtatagt taaattttta ataattgaaa
     5101 agttcaagtg atctttccaa aatacctttt acatatgcta ttaagatacc atagtttaca
     5161 ataggtacat tgaaagattt tgccttttct attctagata acattccagc tctattcatc
     5221 atacaagctc cacagtgaac tacaagggca tatttattta tatcttcagt gaaggatact
     5281 ccagaactaa attcaaaagt aatatttttg ccagttttct ttctaatcat attaggaatt
     5341 tttactgttc caatatcatc agtttgtcta tgatgggtac atccttcagc catcaagatt
     5401 ttatcaccat cttttaaatt ttctaaagca taagctccat taatcaattc tttaaggtct
     5461 cccttttgtc tagcaaacaa tatagaaaaa gatgtaagag gaatatcttt tggtgtatct
     5521 ttatttactt gtggaaatac ttgagagtca gtaataacaa gttttggttt tttgcctaaa
     5581 ttagatagag tttcttttaa actatcttct tttgtaacta tagaaattgc accattatcc
     5641 aaaatatctc ttataacttg ctgttgagga agtataagtc taccttttgg agctgcttta
     5701 tctattggaa caactaaaac aactaaatca tttggttcaa ttaaatctga taccaatttg
     5761 aactcagtgc tatccttagg caaaactttt attatttcat tttttaaatt ttcaattcca
     5821 attttatctg ttgatgagac agaaacaact ggacatttaa ctttattttt agttaagttt
     5881 aaaatttctg attgattctt aatagtatca attttgttga gaattagaat atgagggata
     5941 ttcttatcat taaacttttc aattagtgac aaatcttctt gtgatatacc aatttggcaa
     6001 tccactacta gtaaagctat atctgtcttt tctaaaacat ctagtgattt acctattcta
     6061 agttcaccaa gttcaccaac atcatcaaga ccagcagtat ctataagaac acaaggtcct
     6121 ataggaagga tttccattgg tctaaatact gggtctgtag tagttccagc aatatctgag
     6181 actattgcag cactttgatt tgttatagca tttattatgc tagatttacc tgcatttctt
     6241 ttaccaaaga gacctatatg tactcttacc gattgtggag tagaatttag actcatttgt
     6301 actcaccacc ttaaacttct ttttttgata tactagtttt tacagataca tgaggtatgt
     6361 tgcccaattt accagtcaaa ctattaatag tatccatttc gccaacaact gctatacata
     6421 cgattgagac accttcttct tcgcaaggaa tgcccattct tcctttaata atacctttaa
     6481 aacctgatac aacctcattg aatttaaatt gacattcttt tggctcttcc aaaatagcac
     6541 ttataactgc aacctttttc ataaatatcc tcctaactca gaaaatatgt cttaaacaaa
     6601 tccaaaatca aaaaactttt ctttgtaaag aaaagtttct acaaaagaaa agttattaat
     6661 tacacaagta taatgtgcta tattaaacta tttccttaca gattagataa ctcttttctg
     6721 gtagggttag cttgtaaaaa tttaatttaa caacaagtaa ctgaagaaaa tttttataca
     6781 atttatataa attataataa aaattaacta aagaaaattg aagttactta tataatagta
     6841 gtaaattgaa attaagtcta tagaaaaacc aatttttgta taaaaataaa aattaaagtt
     6901 aattatttgt taagaattga ctttggtaaa atatataatt aattattctt taagcatata
     6961 atttataagt aattttagtg tattttcaat agcttctata tgtgttcttt cataatggtg
     7021 actattgttt gttccaggac caacacatgc tatttgaaca tcttctcccc aacgaatagc
     7081 ttctgaagca tctgaaccat agaaagtaaa tacatcgaca gcgtagtcta aattattgtc
     7141 ttctgcaaca tcgacaagct tgtttcttaa attaaaatca tataatgata aattatcttt
     7201 tgcaactata gttacaccat gttcactaga tgtttgactt ggtccagtag gtgctatatc
     7261 tattgctaca aattcttttg ttttttcagg taaacattta gaaacaccat gtccaatttc
     7321 ttcataatta cttatataaa agttagtagt atgcttaggc gttaaattgt tttctttaaa
     7381 atatctacat atttcaagaa ccattgctac agctgattta ttatctatat atctagattt
     7441 tataaaacca gattctgtag cctcaaatct atggtctagg catacaaaat ccccaacact
     7501 tattcctaaa tctaatacat cttgctttgt aaatactttt tcatcaagtc taataatcat
     7561 tgtttcttca tttctttcag tggcagcttt tgtttgacca tatatatgag tagaagcata
     7621 cttaaataca acacttcctc tgattttttt accttttcta gttataacag tgcagttttc
     7681 tccctctata gctgcccaac atccaccacc aactttatgg tatttaagag taccatcaga
     7741 tgatatttct tttactatag ctccaagtgt atccatatgt gcagatattg ttatttgatt
     7801 tgaatcatct tcaccaggaa gagtagctat aagtgcacct ttttttgtaa gaatagtgtc
     7861 aagacctaaa ttttcaaaat cttttttgat ttctaaaact gcattttcag tatatccagc
     7921 tgggcttgga atgtccaaat attttttcat taaatttata gtattagtta aatttaaatt
     7981 catattgttt cctcctaaat aaatatattt atacaaatta taacataaat acaaatgttc
     8041 tgaaaactaa aattaaaaga atagaatcta aacatattta aaaaaaaagt atataaaata
     8101 aatttgattt atattatata gatttaaagt aaaaatagtt ttatttaata attaataaaa
     8161 ataaatacaa aacaaaataa ataaataata aaatatttag tttaattatc tttacaaata
     8221 aaaattttag taataaaata attgttgaaa aagattttaa attcagatta ttcatttcta
     8281 aagtaattaa tgtgcaaatc tttattaata tatgtcaata gttaggtata tatgtattta
     8341 attagcatga tatagataaa agtatttatt ttaattgtct attatctgaa aaagtcttta
     8401 cttagaaatc ataaaaatca aattaattag gaaaagaaag ggtgtgtata atgtgattga
     8461 gactggattt gtatattgta gtttcttggt tgtattcgta ggtgtaattt tttttatggt
     8521 tgataaattg aaatggaagg tatttgatgt tattaatcca atgcttttaa tatacttagg
     8581 ttcagctata cttggatcat ttggtttatt tgcacagaat gaagatgttg gaacgtatca
     8641 aaacataata acagtgaact tccttccatt aatgttagtg ttcttgttaa ttcaatgtga
     8701 tttaagaaaa atattaaaga tggggcctaa aatgttatta tcatttttct gtgctacatt
     8761 tacatttatg cttggatttg ttatagcatt cataattttt aaaaattcaa tgggtgcaga
     8821 ttcatggaaa attcttgggg cagcatctgg ttcatggata ggtggctcat caaatatgat
     8881 tgcagcagct aatgctctag gagttgcaga agaaggactt agttatgcaa tacttatggg
     8941 ttctatagga tatacagtat ggatgtctgt ttgtctaatg tctgtgaaat ttggacctaa
     9001 atttaacaaa tggacaaagt gcgatacaag ttttatagat gaggttacat caaaacttga
     9061 aaaagaaaat aaagataata cggcacctac gtttgtagaa cttatgattt tactttctgt
     9121 tgggtttgga gttgcgtctt ttgcacaaat tgtatccaag atacttccaa cttcaggtat
     9181 acttaatgaa actatgtggg tagtacttgt tgcatctaca gtatcaatat tattgggtat
     9241 gacaaaaata tcaaagctta aagggagtac actggtagga aatgtgttta tgtatttact
     9301 ccttgcagct acaggtagta aagcaaattt ctttggattg tcagaagcac ctgtttacat
     9361 aatgtttgga ttggtagtag tccttgtaca tgctatttca tttatagggc ttgctaaact
     9421 atttaaactt gatttattta cttgtgaagt aggaagtata gctaatatag gaggagtaac
     9481 tacagctcca atgattgctg gggttcacaa tcctgtatta ataccagtag gtgtacttat
     9541 ggggcttcta ggaaatatta tagggactta ttgtgctctt gtggtaactc aaatattaca
     9601 tatgttagct taataaaaat atggtataaa attaataatt taaataagaa aagtcatttc
     9661 aatttaagca gaagctttta ctgaaatggt tttttttgtt tgacaagata atctatttca
     9721 agaagatgtt ttgacatatt tagtgtataa tattgtaaaa tttactaaaa acgagatata
     9781 tttaattttt atattgaatc gaacgtctgt atgatgtata ataaatatac agaacgaatg
     9841 tttgcagaac gggtgttttt tgaatttatt ttaataattt atgaatatat atcagaatgc
     9901 ataaatatat ttaaatataa agattaaaaa gaatttaaag gagtgttgat aacttatgat
     9961 aattccatca agaagaatac aagatataac attaacaaat ttaaaaaatg gtgaagtaac
    10021 tttaattgaa ttggatgaaa tatacaagaa gatgggattt ttatttgttg ttagtgaagg
    10081 caaattgaaa aagataaaaa aggagaatca tcattagaaa tgaatgaaat aataaatatt
    10141 ataatagttt ataaaaccta acttatgtag aatataaata cttaagttag gtttttttgt
    10201 gataaagttt ataagtactc aattttataa gataaataat ggtcttaagt gaaaagtaaa
    10261 taagtttaaa ttttagtaag tattatattg cataagaggt gatattatta tgaaaatagt
    10321 aacctacaat attcataaag gtatggattc aaataataga ttaactctaa ctaaaatggg
    10381 gttatattta aaacaattgg attgtgatgt aatttgtttg caagaagtat tatatcctca
    10441 atttttagct ttaaaagctg ttttaaatat ggatggtgtc tttgctacaa atgtaaaaaa
    10501 agtaaatatg atatatggta tttgtacatt tacaacatct aaaatgttaa acagtaatca
    10561 ttttttctta actagcaaaa aagaacaacg gggagctctt tgtataacta tagatgcata
    10621 tggaagaatt ataaatgtaa taaatactca cttgggactt gataggcagg agagagcaaa
    10681 acaattagac gaaataatag actataggaa cagattagtg gggatagtta ttttatgtgg
    10741 tgattttaat gagaaaaatg tctttttaag tatgtttaat gatatggcta tttcactaaa
    10801 caagtcttac ttaccaacat ttgaaaaatc aaactctaga atagattaca tttttgttaa
    10861 taaaaatact gaattaaaag gatacacagt agaaaaaatt tacctgtcag accattatcc
    10921 tgtaataggt tacatttaaa tttcaatata aatgaaatta gttgttataa aataaacaat
    10981 tttttgttaa gaaatagtaa aagaaatata ataaaaaata tcaaaaataa aactaattat
    11041 aagacataaa tttacaataa agttacattt gattaaattc cttgtttaac aaaagctatt
    11101 caaatataat ctaaggtagt tttgaaatgg gaactctatt atgcaaattt aagctagtgt
    11161 caaaatgagg tgaagttatg ggcagaagaa caacaagagt ttcaagatta ggtaaagaaa
    11221 aaaaacgaag tgttttaatt gtattggcag ctttagttct aataatgggt ggatttactt
    11281 atttttttaa cagtaaattt ctgtacaatg ggaaaatcgc taaaaatgta tatatagaag
    11341 gagtaaatgt atctgatatg actaaagcag aggcattaaa agctataaca gataagtata
    11401 ctccagaaga cttaaatctt acttatgatg ggaaaaaata tacaatatca ccaaaagata
    11461 ttgatttaaa atatgataca gaagatgttg taaaagatgc ttatgaaagt acaaaaaaag
    11521 ggtcctattt tcaaaactta aaaaaatata ttgatataag agtgaataag gcaaatatga
    11581 agataaaagc tgaatatgat gaggcaaaac ttagttccaa agtgtcttca atagcagata
    11641 gtataaatgt taagatgaaa aatgcttcta ttagtgttgg aagtggtgga ctaagttata
    11701 cagactctgt tgtaggtaga gaatttgatt tggcagcaaa taaagagtct atatataata
    11761 tgataaaaaa taaagagcat aaaactttag aacttaaagt aaatcttcaa aaaccagata
    11821 taacaactga acaagtaaaa actgtaaatt ctgttatagg tcaatattca actacttatt
    11881 cacaagctgt agagggtagg tcatataatg taggattatc agcaagaaaa actagtgatg
    11941 tattattaat gccaggtgaa gaattttcat acaataaact tacaggacct agtaataaag
    12001 caaatggtta caaagatgca cctgttatag tttatggtaa gctagaacaa tcagcaggag
    12061 gaggtgtttg tcaaacatct tcaacagttt ataatgcggc tcttctttcg ggtatggaga
    12121 taactcaagt tacaaatcat tcatctgcat ctacttatgt gcctaaagga agagatgcta
    12181 ctgtgagcga tggaggatta aacttaaagt ttaaaaatcc atataagcat cctgtatata
    12241 ttaaaaatta tgcaggtgga ggaagtgtat catcagtaat ctatggtaat tctggtgaca
    12301 aaccaaatat atctatagag gtaaaacaaa ctggtcagaa taaatattct acatatcgaa
    12361 tatttaaaga ttcaagtgga aaggtcataa aaaaagaaca tatatcaaat agttcttata
    12421 aagaattaaa aaaatagata attttttaga gaaatatttg taatataaaa gggagtgtct
    12481 caaaatgaac tttttagttt atgagacact ctttttatgt gaaatcaaat atattttcat
    12541 catttcaaca atacaaaaga agcgtatctc tattttgaga cagccttttt tgtaaaaatt
    12601 aacactatta gttcttgttt taatattaca atcattaata tatagaaaat attcctatat
    12661 attggtataa ttgtaaatag atttagttat ataaaatgta tgaggaggtg acatggttga
    12721 attttatagg caatagatat gaggtagtaa attctgatga cgttttagaa attaacaaaa
    12781 tctataaagc tagagatgtt ttttacagga gaaatgtgtt aataaaagtt ataaagcaca
    12841 ataattatat ttgtgaagat tttgtttcaa atttaattga tgaaagtact gctttagatg
    12901 agataaactc tccttatata ctaaaaataa tagatgttgg gattcactgt acagaagaaa
    12961 caacattata ttatgttgta agtgaagact ttaatggtat cggactagat gaattaattc
    13021 taggtaatta tcttcattta gaagcaattg taaacataat gatacaagtt ttaaaagctt
    13081 tagaaatgat acataggaat cattcatatc atggtagttt aaaatcgagt agtataatag
    13141 tagatacaga atacaatata aaaatttgtg attttggaat tacaaaagca aataatgggg
    13201 ttaatacaag aagctatgga aacaggagat atttatgccc acatcaacta tgtataaatt
    13261 atactgataa agaaagtgat ttttttgcaa caggtcttat tctatttgaa tctatattta
    13321 aaaaattgcc ttttggagaa tcaaattcag aagaaaaaat gctacaatct attgataagg
    13381 gattagattg gaatagtata aaagcaatta atggaaatac agaacttata aatgttataa
    13441 aaaggcttct tggaagaaat aataagtata ctagagccaa tgatattatt attgatttaa
    13501 gtaaagtaat gtatgaaaaa gcatatatag aagttgaaga tgagaaagaa gaagaacaga
    13561 aacaagacaa agttatacaa gttaaaaaag aatataaaag taaaatgaga aaaaaattac
    13621 ataaaaaact tgttgttgcc ggtctagcta tagtcatgat ttcaatgatt attattagtt
    13681 ccataatata aaaattgtat attaagtatt ttaaaatcaa tgtctataaa tgatatacat
    13741 agaaatttta gtgtagtctt aaattatgaa aggaagtggt tattatgtta acaaaaaata
    13801 atgaggtatt aaacgtctag gagtatttta acaaatagcc acctgctttt acagtttaga
    13861 ataaaaaagc tgtggctaaa ggtcacagct ttttgctact ttttaaaggc aggagaatat
    13921 taaaaaggag caaattatga gcaatataaa aataatagca agtaataaat gttggattga
    13981 agatatagca aggaaacaac tagaagatat ttcaaagtta aatggaattt taagagtagt
    14041 aggattacct gacttacata caggtaaaat acctgtaggt ttagcagttg agactaaaaa
    14101 tattatttat ccacatataa ttggaaatga tataggatgc ggaatgactt tatttaaaac
    14161 tggggttcta aaaaagaaat ttaaaaaaga caaatggatt aagtcactta gtaagattaa
    14221 agatttgtcc gatatagaaa taaaaaacac atataaagaa gaatgtccaa ttttaaattt
    14281 aggaactata gggagtggaa atcattttgt agaaattcaa tgtataagtg aaatttataa
    14341 taaagaacaa tttgaacaat taaaattttc atctgatgat attatgatgt tagttcactg
    14401 tgggtcaaga aattatggag aagatatttt aaaaaaattt tatgataaag atggacttga
    14461 agttgaaagt gagaaagcaa tagattatat taaaaaacat gataatgctt taatgtgggc
    14521 agagcgtaat cgtgaagcta tcagtaaaaa aattatgcag tcaataggta catctgaaga
    14581 tgttgaaaca atattctcta taaatcataa ctttatagag aaaagggaag ataaatttat
    14641 acatagaaaa ggtgctgttt caagtgaaag aggagctgtg attataccag gttcaagagg
    14701 cagtctatca tatatagtta tgcctactga aaatacagag atatctttat attctctatc
    14761 acatggagca ggtagaaaat ggtctcgttc agtttgtaaa tcgcgcctta aaagtaagta
    14821 tagtaaagat actataaaac aaactaaatt taaaagtcag attatatgta atgatgtaaa
    14881 tttattattt caagaagctc cagaggcata taagaatata gagcagatta ttgaaagttt
    14941 gatagaatat gaacttattc aagtagtggc aactatgaag ccattgatta cttataaagg
    15001 ataattttta aagttaaaaa agattaaaat tatattataa atagctaatt ttatggaaca
    15061 tttaaaagtg ttttattatt attagctatt tttttatttt atagagacga taatttatag
    15121 agctaaattc tttgatatat aatgattaca aagttagatt gtattggaaa ctacacatac
    15181 atgaattgat gacaatataa gtaattccaa aatataaatg aaagtataag cttattttca
    15241 ctataaaaat tttcactgta caaaaacatt aataacatta tttttattga cataaatata
    15301 taatttatgg aataatatta gaagcactgg tataagtgtt taaataagaa tagaaaagga
    15361 gaagtgtttt atgaaaaaaa atactaacat taatgagtcc ggagttaaac ttcttaataa
    15421 ataaggagtt tgatatataa taactcctta tttattaagt tgttcgcaaa ataactatta
    15481 tcaaagtaaa tgatagggtg atttaaaaga cgatgactta taaataaagg agaaaaaata
    15541 atgaatagta atacatttga aagattgcaa ctaaatgaag ttaaagactt aataaaaata
    15601 cattgtgtaa gttcgctagg aaaaaattta atagataaat taactcctag tgggaatatt
    15661 ggagtagtaa gaaggaaatt agcagaaaat aaagaagcta gaaagataat tgaaaatagt
    15721 aatcacatac cacttgaagg attgtttaat gcaggttcta caatagataa aattgaaaaa
    15781 ggaatgataa tagaacctgt tgaacttgta aatattgaag attttttaag aggttgtagg
    15841 aagatgaaag cttttatgtt agaaaaagaa ttttattcac ctacattaag ttcttatgct
    15901 ttaaatataa cagaatgtaa aagtatagaa gatgagataa attactgtat aaaatctaat
    15961 aaagttgatt ctaatgctag taaagaatta aaaaagataa gacgaaatat agaaattaca
    16021 gaaggaaaga taaaagatag attaaataaa tttataacat caactgtaaa taaaaagtat
    16081 atacaagagt ttataataag taaaagaaat gatagatatg taataccaat taaatcttct
    16141 tataaaaatg aagttaatgg tactatatta gatacatcat caaaaggaaa tactgtattt
    16201 attgaaccta ttagtgtttc aaatttaagt acagagttaa ctatgctaaa agctgatgaa
    16261 actattgaag aatacaaaat attatcttat ttaacagaac ttatatttga gaagataagt
    16321 cagataaaat taaatataga gatactttca gaatacgata tggtatttgc aaaagctaag
    16381 tatagtcaaa aaataaaagg aattactcca aaaataaata ataatggcta tataaaaata
    16441 attaaaggta aacatccact tctaacagga gatgcagtac ctttagattt tgaaataggc
    16501 aaaaattata gaagtcttat aataacaggt cctaatgcag gaggaaaaac tgtaaccctt
    16561 aagactgttg gtttactaac tttaatggtt caatgtggat ttgatatttc tgcaaaagaa
    16621 ggtagtgaat ttagtgtatt tgaaaaagtt tttgtagata taggggataa tcaaagtatt
    16681 gaaaatgcct taagtacttt ttcatcacat ataaaaaata ttgcagagat tatgagttta
    16741 tcaaataatt ctactcttgt tctgtttgat gaaataggaa gtggaactga acctaatgaa
    16801 ggagctggtt tagcaatatc attattagaa gagttttaca aaatgggatg tataactata
    16861 gcatcaactc attatggaga gataaaaaag tttgctacat tacatccaga atttgaaaat
    16921 gctggtatga tgtttgataa agaaacgtta gaaccattgt ataagttaac tattggaaaa
    16981 tctgaagata gtaatgctct ttttatttct aaaaaaatgg gaataaaaaa tagagtttta
    17041 gatagagcta aagaatatgt aattgataga aactacaatt tagatttagt taaaagaagt
    17101 aagctacaat ctaatattga agataaaaat agttctgaag tcattttaag ctatacagat
    17161 tataatgtag gggataaggt aaaattacta gatgaagatg attttggaat tgtatataag
    17221 cctatggaca agtttaataa tgtagaagta ttgtttaatg ataattttat ttcagtaaat
    17281 ataaaaagat tacaacttag cataaaagca tcagaattgt atccagaagg gtatgattta
    17341 gatgtattat ttactagttt taaagaaaga aaattagaaa aagatattca aagaggttca
    17401 aaaaaagctc ttaaaaagat tcagaaagaa attagagaga atagaaaata aatataaaaa
    17461 gcacaaaaga aagcaatcta aagagaaaag tttatcttta gattgctttc ttattaacat
    17521 atcttcataa atccaatgac taacaagatt aaaccactta accaagataa gtttaaattt
    17581 acgtttttaa caaacatatt tcctagtaaa caaccaagta atactgcaat tatatttgat
    17641 attaaagaaa atagaataac ttgtatataa ttcacaccta caaggccact accaaaaccg
    17701 atagctaatc catctaaact taaagcaagt gcaagtgtaa atgcttcagc agatttaagc
    17761 actttagatt tacttgtatc taagacatca ggttcgatac ttacattaaa atttaattca
    17821 aggtctaaca tattaaaatt ataattcttt gagtgtatag attttttctt aaaataagat
    17881 ttaaaaaaac tttcaattag tctagcactt cctaataaaa ataaaataaa gaaacatatt
    17941 aacattgtta tattttgagg taaaaaatct ctaactattg tacctgcaaa aagcgatatt
    18001 gctaaaatag ctgaacaaac tacgctgata attatatttg aatataaggg aactttaact
    18061 ttatttactc catatccaaa gcttgtcaca aaggcatcta tacaaagaga agtaactaat
    18121 aaaattgatt caatcacagt agattaacat cctttctctt aaattttgta cttatattag
    18181 ttatagtatt gtaaaaatgg ataaattgtt acagataatc caataaattg ttgataaaat
    18241 ttcaacatca tacaacacct aaataacaaa agaatcatat catataatat aaaaccaatc
    18301 ataataaagc aggtgaaaat aatggaacaa catagtaagg ttgggaattt cggtgatttg
    18361 ttgaaagttt tattagatga aaaagaatta tcaatgggag aactaagtaa aaaaagtgga
    18421 attgataaat ctactatatc tcgtatagct aataataaac aaaggccaaa tataaatcat
    18481 ttagaaaaga tggcaataca tttaaatatt aacctagaag aactgttgaa agcttcagga
    18541 tatgaattta gaaacagcaa taatcaaata tttaatgcag atagtgattt tagtaacttt
    18601 gatgatattc ttggatttgc aaatttaatc aatgataaga actttaatgg aaatattgaa
    18661 aaagagttaa gtaagtgtaa gttatatgtc caaacagatg aaggtaaaaa attacttttt
    18721 gataatttta ataaaaaaat agattctatt gaaaaacaag gtcaatttac agatagatta
    18781 agacaaatgt atgctgattt ttgtacagat ggacttacaa taaagaaata tttattgatt
    18841 ggaagtatgc tactttactt tgttatatca acagatgtga ttccagactt cgtgttccca
    18901 ataggattta tggatgattt agtagcatta aatatagtta caaaattact taaaaatgat
    18961 aagtaagttt tagaataata tttaatatat aaataaatca aagtatataa aatttattat
    19021 tttttatatg aaacaaatta
//
LOCUS       DAELXX010000076        18808 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.076, whole genome shotgun sequence.
ACCESSION   DAELXX010000076 DAELXX010000000
VERSION     DAELXX010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18808
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.076"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            317..1534
                     /locus_tag="KPH29_002524"
     CDS             317..1534
                     /locus_tag="KPH29_002524"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HBG1468110.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1629..2009
                     /locus_tag="KPH29_002525"
     CDS             1629..2009
                     /locus_tag="KPH29_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBG1468111.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2293..2775
                     /locus_tag="KPH29_002526"
     CDS             2293..2775
                     /locus_tag="KPH29_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG1468112.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSNVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVNLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            2796..3611
                     /locus_tag="KPH29_002527"
     CDS             2796..3611
                     /locus_tag="KPH29_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG1468113.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3662..5212
                     /locus_tag="KPH29_002528"
     CDS             3662..5212
                     /locus_tag="KPH29_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG1468114.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGCIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPLLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSIIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            5309..6661
                     /locus_tag="KPH29_002529"
     CDS             5309..6661
                     /locus_tag="KPH29_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG1468115.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKERQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAESLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKVFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            6792..7550
                     /locus_tag="KPH29_002530"
     CDS             6792..7550
                     /locus_tag="KPH29_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1468116.1"
                     /translation="MNIINKIFWQEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDND
                     GLEKLRQQGTFLIYNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELLNLLRTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            7597..8316
                     /locus_tag="KPH29_002531"
     CDS             7597..8316
                     /locus_tag="KPH29_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBG1468117.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8440..9840)
                     /locus_tag="KPH29_002532"
     CDS             complement(8440..9840)
                     /locus_tag="KPH29_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBG1468118.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADIGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(9844..10752)
                     /locus_tag="KPH29_002533"
     CDS             complement(9844..10752)
                     /locus_tag="KPH29_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG1468119.1"
                     /translation="MKGDNKVTEKQRLIINIAQKIFDQKGFQNTSISDVVKECKMSKA
                     TFYKHFETKESFICEIINYYDEKFLEIIHSINENNDIPSSEKLNRKIIAVWKNIFSRT
                     TINTYIRENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDA
                     LIHQFIYIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYF
                     SLHNKSLFFKTIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAY
                     LEKEDTLRPKVLLLNSIKNQLKKETL"
     gene            11013..11660
                     /locus_tag="KPH29_002534"
     CDS             11013..11660
                     /locus_tag="KPH29_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG1468120.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            11985..14624
                     /locus_tag="KPH29_002535"
     CDS             11985..14624
                     /locus_tag="KPH29_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBG1468121.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHTEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMDTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14682..15785)
                     /locus_tag="KPH29_002536"
     CDS             complement(14682..15785)
                     /locus_tag="KPH29_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HBG1468122.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSSK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIPLSSIENIVKEAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
     gene            16146..16967
                     /locus_tag="KPH29_002537"
     CDS             16146..16967
                     /locus_tag="KPH29_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG1468123.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    17046..17257
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            17344..18660
                     /gene="argH"
                     /locus_tag="KPH29_002538"
     CDS             17344..18660
                     /gene="argH"
                     /locus_tag="KPH29_002538"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBG1468124.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVGLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELHKFSDAFKEDIYDFIDYESILNKGIKKNLK"
BASE COUNT         7206 a         2158 c         3316 g         6128 t
ORIGIN      
        1 cttaataatg ttaaataaaa tcaaaaaaat agattttggg taaataaaat aatataaaat
       61 ataaatttta ataaaaataa taaagtatat attattgctt taataagaat gtaaaaaata
      121 tttttaaatt atcatttaat gtaatttaaa tgaattgaag atatatgttt attgtatgca
      181 attgttgtat ttttaggttt tttgtgatta attaaaaaat tataaaaatc gtttacatgt
      241 tgatttttaa aaaaaaaaga tatagaatta tatagaaagt aataaacaaa aattggattt
      301 acgggagtgg aatgaaatga tgaatgttac cttaacagat gtgaaagagg ctagagagac
      361 cattaagaac atagtaaaaa gaactgactt acttgaaagt gttaaactta gcgaaaaaac
      421 aggagcaaat gtgttctata aatgtgagaa tcttcaaaaa acaggttctt ttaaattaag
      481 aggagcttgt aacaaaatag cgagtttaac agatgaagaa aaggcaaatg gagttatagc
      541 ttcaagtgca gggaatcatg ctcaaggagt tgcattggga gcaaaaatga ctggtataaa
      601 atctacaata gtaatgccag caactgcgcc tttagcaaaa gttagtgcaa ctaaaggtta
      661 tggagcagaa gtagttttaa atggtgcggt ttatgatgat gcatatgcaa aagctgttga
      721 aatacaaaaa gaaacaggag caactttctt acatccattc aatgataagt atgttatagc
      781 aggacaagga actatatctc ttgaaatttt cgaacaatta gacaataaag ttgatactat
      841 actatgccca gttggtggag gaggaatcat ctctggtgtt gctgttgcag caaaagcatt
      901 aaatcctaat gtaaaaatag tcggtgttca aactgcaaat ataccttcaa tgaaagagtc
      961 aataaaaaat ggaaaagtaa caacagcatt taatgatact acaatagctg atggtatagc
     1021 tgttaaaact ccaggagatt taacttttga aataataaat gaattggttg atgaaatagt
     1081 agtggttgaa gagacagaaa ttgcagaatc aatattattt atgatggaaa gtcaaaaaat
     1141 agtttcagaa ggtgctggtg ctgtatgtac agctgctata ttaagtggta agtatgtacc
     1201 agcaaaagat gaaaatgtag tttgtataat atctggtggt aatatagaca taaatacgtt
     1261 atatagaata ataggtgttg ctttagctaa agaaggaaga agatattcat ttagtacaat
     1321 tatggaagat aagccaggaa actttgctga gttaactaga ataataagtg agaatggtgg
     1381 aaacatttta agtgctaatc aaggtaaatt atcagcaggg gaagctctag gaaaacaaag
     1441 tgcggaattt atattagaga caatagatta tgaccatata gctagaataa agaaagctat
     1501 agaagaaaaa ggttttaaaa ttatagaatt ataataaaaa gtgtatataa tatacaagaa
     1561 aaatagtata atagtgtatg aagatacatt ttatgtgtat tagattaacg aattaggagg
     1621 attttaaaat gaaacatgaa gtaatacata ctaatgatgc accagcagct ttaggaccat
     1681 attctcaagc tataaaagct ggaaacttat tatttgtatc tggacaagtt cctcttgtac
     1741 cagagactat ggaagtagtt gaaggagatg tacaagctca aactgctcaa tctttaaaaa
     1801 atcttaaagc tatattagct gaaagtggag ctgatttttc aaatgtagtt aagactacag
     1861 tatttataaa agatatgaat gaatttgggg ctataaacga agtttatgct gaatattttg
     1921 gagaaaataa accagcaaga gcttgtgtag aagttgctag attaccaaaa gacgttaaag
     1981 ttgaaataga agtaatagct gtattataaa aatacaatat ttttaatttg aaaaggctga
     2041 aaaactatat tctagtgatt cagtcttttt ataaaaaaat aaatcacaat aaagaacatg
     2101 ataaatattg tcgaattaaa aaataaaatt aaaataaata ttgcaatatt tttgagaagg
     2161 ttttataatg agtatataat ttgaaacgtg taactattaa attaggcatg agttcttgga
     2221 tatttttatc ctgaggattc atgccttttt tgtattgaaa ttcaaaaatc tatttaataa
     2281 ggaggaagta ttgtgttaaa ttttttcaag aaaaataaaa gctataagtt acatgcagta
     2341 gtgagtggta acagcattaa tattgagaaa gtaaacgatt ctgtgttttc aaaaaagtta
     2401 atgggagatg gagttgcaat tattcctaat agtaatgttg ttgtagcccc ttgtaatggt
     2461 aaagtaactg tgcttacaga atcaaagcat gcctttggta tggtctcaga tgagggtgtt
     2521 gaaatattag tccacatagg aattgataca gttaatttac agggtgaggg gtttaaaaat
     2581 gaagtgtctc aaggagatac tgttaaaaaa ggtagtccaa taataagttt tgaaagagaa
     2641 aaaataaatt ctcagggaat tgattgtaca acaataataa ttgtattaaa tcatagcgaa
     2701 ttttcagaaa taaattgcat ggtagaaaat gaagttgttg cagggcaaga tactgttatt
     2761 gaaattatga aataatagga agggattaat tcaatatgag agtagtaaaa atctttaata
     2821 ataatgcatt atctacagta actaatgata ggaaagaagc tattttacta ggtcttggta
     2881 taggttttaa taaacgtcca ggagataaag ttaatgaaga taaaatagaa aagatttatt
     2941 atgttcaaga ccacatgcaa acaaaatttt tagaattatt aaaaaatgtt agtccagaag
     3001 tgatggatgc ttcacaacaa attataagtc ttataccagg aaatgaggga aaatttaata
     3061 ataaagggat attatcttta gtggaacata ttagttttgc aatagataga atgaaaaata
     3121 atgttttttt acctaattta atgcttttgg atataaagat gatgtattca aaagagtttg
     3181 aattaggagt aaaagcttta gaaattatat atagagtatg tcatatccat ttgcctgaag
     3241 atgaagcagg ttacatagct ttacattttg tcaatttaca atcaaatgat aatttggcat
     3301 atgatacttt aaaatttgta aaaggtagta ttgatttgat taaagaatgt tatggattag
     3361 aattagacga atcgagttta agtacattac gttttagaac tcatcttaaa tttcttgctc
     3421 aaagaatttt taagaatgaa atttgtcagg atgataaaat gattgaaatg tatgattatt
     3481 taattaacaa tcatcctaaa aataaagaat atttagaaaa gttaaatgta tatattgaaa
     3541 aggagtttag gtataaatta gataaacctg aaaaaattta tctactacta cacctaacaa
     3601 aaatcttata agaatgagat gaattagtaa tagaaatata taattttagg gggggataag
     3661 attgaaagaa aaactacttg gaggactgga aacatttgca aaggcaatgg ttcagccatt
     3721 aatgtattta tcgagtgcag gaatattaat ggtggtaggt gtactactta caaatgatat
     3781 gttattagga gtacttccat tttttaaatg ggagccaatt caaatatttg gaagcctaat
     3841 ttataactgt ataatggcta ttattaataa tcttgctgtt atatttgcta taggtattcc
     3901 tgctgcacta gcaaaaaaag agaagcacaa agcagcgtta ataggattta tgagttatat
     3961 aatatacctt acagcttcca acactatgtt aacatcacta ggtcaactag caaaagaaag
     4021 caagatgctt ggactgatag ggactggaca agctactatt ttaggtattc aagttgtaga
     4081 tacaagtgta tttggtggaa ttattttagg gtgtatagct ggatatgtat ttaatcgtac
     4141 aagcaacaaa acatttaaag gagctttaca gatatactcg ggagcaaatt ttagttttat
     4201 atgcatgttc tttgtatcca ttttaattgg agttgtgagt aactttatat ggccattagc
     4261 tcaacaagga atagcttctc ttgcaaatgt catcaaagat acaggtacat ttggtttatt
     4321 cttatatggt tttttagaga gaatattagt accaactgga cttcatcatt taatctatac
     4381 accatttcag ttttctgatt taggaggagt gcttaaagtt ggtgcagata cagtttcagg
     4441 agcctatgct atagttatga cagaaatgaa tttaccagtt gagcgtttct cagatagtat
     4501 atactacatg gcaactggat ttacaaagat gtttggttat ataggtatag gagctgcctt
     4561 tatttatact gcatataagc aaaataagaa gaaagttaag gcaacgatta ttccactaat
     4621 aattacggca gtattagcct ctgtaacaga accattagac tttatgtttg tattctgtgc
     4681 accactttta ttctttgtac atgcagttat agctggtctt tttatagctt tattaaaaat
     4741 atttgatgtt actgcattat gtggagggaa cttattagta tcaattataa tgaatactac
     4801 agtaggtgta gaaaaaacac actggccagt aatgatgata ttaggacttg cacaaattat
     4861 tgtatatttt gtagtattta gttttttaat taagaaattt aattataaaa ctccaggtcg
     4921 tgaagatgag agtacagcta caggcgaaga acaatcaaaa caacttaatt tagatgcagg
     4981 tatagaaaat ataattgatg ggttaggcgg aaaagaaaat attaatacag tagaaaattg
     5041 catcactaga ttacgtgtta atgtaaaaga tgaaagtaag attaatgaag atataattaa
     5101 tttaacgcca aatagcggga ttgtaaggaa aggaaaagat atacaaataa tttttggact
     5161 tcatgtgcat gaggtaagac gtgcagtaga agatttttta gaagcttatt aaagatatat
     5221 atttatatag caagggtaaa taacagcttt aagtgctgtt tactatttat taaagataca
     5281 tataatttat agaggaggaa taattattat gattattact attgttggtg gaggaagcac
     5341 atttactcca ggtattgtta agtcaattgc gttaagagaa aaagagttag agcttgagga
     5401 aattcgctta tttgacattg ataaagaacg ccaagataaa gtagctgtag ttgtcaactg
     5461 gatactaaaa gaagatgtta aaacagatgt taaactaact gtaactaatt gtgaaaaaga
     5521 agcgtataca gatgctacat ttatatttgc acaaatgcgt gttggtaaat atgaaatgcg
     5581 tgagcaggat gaaaaaattc cattgaaata tggttgtgta ggtcaagaaa cttgtggttg
     5641 tggagggatg gcatacggac tgcgtacaat acaaccaatg ataaaggtta ttgatgatgt
     5701 agagaaatat gctaaaaaag attattggat attaaattac tctaatcctg ctgcaatcgt
     5761 ttctgaagct tgtcgtaagt taagaccaaa tgcaaggatt ataaatatct gtgatatgcc
     5821 aatagctatt atagatatga tagcggagtc attaaatatc aatgacgttc ataatatacg
     5881 ctatgattat tttggtctaa atcattttgg ctggtttact tcaatagatt ataaacatag
     5941 agacttaatg gaagagatta aggaatacat aaaagaaaat aaaatattat taccaaaatc
     6001 ttatatagaa aacttaaata aaagtcgtag tacaggaaat cgccatgctg taagtagctg
     6061 gatacatgtt tgggaatctg tttatacaat gttagaaagc ttcccaaaat atctgccaaa
     6121 cacttattta acatactatc ttttaagtaa agaaacagtg gaacattcaa acataaatcg
     6181 tactcgtgca aatgaggtta tggaaagccg tgaaaaagac ttatttgaag gtgtgagaca
     6241 ttacttagaa actggagaaa tttctgaaaa agtattttat gcaggttcac atggagattg
     6301 gattagtgat ttggcagttt caattaaaaa tgatacacgt agtcgttttt tagttataac
     6361 tgaaaatcgt ggagctatac caaacatgcc atatgatgca atggtagaaa ttccagcata
     6421 tattggtaaa gatggtccag aagttattgc tcgaggtcca atacctctat tccaacaagg
     6481 attaatgatg caacaactta acagtgaaaa attgatagta gaagcttata tagaaaatag
     6541 ttatgaaaaa gctttacaag cattcacatt aaataaaact attccaagta tgaatgttgc
     6601 taaagaaatt ttggatgaaa tgatagaagt taataaggaa tactggccag aattaagata
     6661 gtaggataaa agaaagggtt tttatttaat aattagatat gttttcagag tcttttatca
     6721 aaatgtattc acaaacatat ggcaatatat taaaataaag agtgatattt ttagaagaat
     6781 ggggtgatgc aatgaacatt ataaataaaa ttttttggca agaagatagg attggcatga
     6841 ttacaaataa tttagaggca gatacacata gccactgcat gttacaactg ttcttaggga
     6901 tagaagacag cattgaaata acagtcaatg aaaaattagt taaatgtaat tgtatcatag
     6961 tagataaaaa tatctctcat tcattttcag caagaaaaaa agtttattac tctgctatta
     7021 ttgaacctac atctatatat gcagaacagc ttacctcaaa aatgaatgat tttggctact
     7081 ggatatgtga caatgatgga ttagagaaat tacgtcaaca aggaactttt ttgatatata
     7141 actctagtaa agaacaatat ttaagattta tggaaatgct taataactat ttgaatatac
     7201 ctataactct caaacattat gatgacagaa ttactgaatt gctcaattta ttacgtactt
     7261 gtaattgtga taatcatact atttcaagtt ttgcagataa agtgtcccta tcagcaagta
     7321 gattatcaca tctgtttaaa gaacaaatag ggataccttt aaaaagctat atcttatttc
     7381 atcaaatgga gtgtgctttt agagaattac tttctgggaa aaatgttacc gaagcttcta
     7441 tgactgctgg attcgatact ccatcacact ttgcagggac agttaagcga atgatgggaa
     7501 tgcctgtttc tttatctttg aaagatagcg aatttttgaa agtatattaa atattctcgt
     7561 tatataatga agttgtacta agaaaggggc tttgttatga ataaagaata tttagaggaa
     7621 acaaaaatgt taaattataa tgaaccacaa cttaagcttt tggtatcatc aaagaattgg
     7681 ttggaattag atgattttca taaaataaaa tctatttatg agtttgtaca gaatgatatt
     7741 ttatttggat ataatgcttc tgatatgtta agcgcaacac aggtattaaa tgatggtatg
     7801 ggacaatgca atacaaaagc tactctttta atggcattat tacgtgcagt gaatatccct
     7861 tgtaggcttc atgcttttga tgtaacaaaa gattttcaac gaggtgctac atccaaattg
     7921 atttcactat tagcaccaaa atatattctt catacttggg tagaggtatt ttatcaagat
     7981 aggtggattg ctctggaggg tgtcataact gataaaaaat atttagaagc aattcagaag
     8041 aagtttttta atcatggtgg aacgtttaaa aagtatgcaa tagctacaaa tgatttgaaa
     8101 aatacatcta ttgattggga tggtaaagat acttttatcc aaaaagaagc tatcgtgtat
     8161 gattatggta tttttccatc accagatgta tttttttcta ctcattcaca acatatgtca
     8221 aaattaaaaa atttcatata tgtacatctt atcagaaaaa taatgacaaa aaatgtatgt
     8281 aaggctagaa acaattatat tgataaaaac gaatgagtgt ttaagaggta actatatagg
     8341 aaatatatag aataattaaa actgtactgg ttaaaatgaa taaaaaggag ttatcataga
     8401 gcaaacttaa attgttcata ggataactcc ttttctgtat taacttctaa taatttgatg
     8461 tctttttata aaacatatag atacaattaa accagtaaat gcaagagtag ctgctattga
     8521 aaatgcaaca tctattccat gaatgttaga cagcatgttt gaactcatat gagctttctt
     8581 agtagcactt gtcattatag taataagtat agcagaacct atagctccag atatctgacg
     8641 gagtgtgtta ttaattgctg tggcatgaga tataagttct ctatctaatg ttttaattcc
     8701 ccatgtagtt aatggcatta aaaccattga aataccaatc atacgaatac aatacatcaa
     8761 tgacacatga attaaagatg tatctctgcc taaaaaagcg aaggataaag ttcctaaaaa
     8821 caaacatcct gttcctaata ttgatagagc atgaggtcca tatttatcta aatgacgtcc
     8881 tgcaattgga ttgagtacaa ccatcaatat agcaccaggt aacattaatg aacctgaaga
     8941 taatgcagaa aatcctctta cagattgaac gtacagagga atcattaatg tagcagacat
     9001 cattgaggca tatacaatca taattaatat tgtagaaata agaaatggtt tatttttaaa
     9061 aaccctcaat tctaaaaaag gttctttaga ttttaattga cgcaatgtaa ataaaattaa
     9121 tgacataatt cctattaata ttggtaaata tgtggcaata ttagtccacc cataattacc
     9181 ttgattagta aatccaatta ataaaccacc aaaacctaga gatgatagta ctattgaagg
     9241 tatatctaac tttagttttt gagtttcccc aacatttctt aatagaataa atgatagtat
     9301 aacatctaat atagcaatag gggaaagaaa cagaaataaa ctatgccaac caaatgaatc
     9361 tactaaccat cctgataaag ttggacctat tgctggagca aatcctactg taataccaac
     9421 taaactcata gctgcaccac gtttttcctc agggcataaa tagagtataa taacttgtaa
     9481 aagtggcatt agagaaccag aacccattgc ctgcaacaca cgagcaatca acatagttga
     9541 aaaattatta gaaaaaagtg caataataca tcctatagaa aagatagaca ttgatgtaat
     9601 aaataacttt ctagtactaa atcgatttat taaataagct gttgtgggta caataatacc
     9661 tgcaataagt agataaattg ttgttaacca ctgaccaata tcagcagtaa ttttaaaatc
     9721 tgacattata ttaggtaatg ctgttgacaa tagtgtttga ctgagagaac ttataaaagc
     9781 accagtcatt acaattgcta ttattaaatt tcctttttta ttgttacttt gggtttccat
     9841 aatttataac gtctcctttt ttaattgatt tttaatgctg ttcaataaaa gtacttttgg
     9901 tcttaaagtg tcttcttttt ctaaatatgc aatcattgca tccataatta aagaatcata
     9961 ttgttgattt tttccctcga cgtataattt ttttattgct tctagtaatt taggattttc
    10021 aaggagagaa gtatcagttt tgattagttc ttctatatct tgtatcgttt taaaaaatag
    10081 cgatttatta tgaagggaaa agtaaacttc atcttcttgt ttatgtaaaa acattgattt
    10141 ttcaattaaa ggatttagat tatccatatt ttctacaact aaatctagaa tttgtatagt
    10201 aaatttggca ataaagtata catttatttc tcttttttgt acatgaataa tgtatataaa
    10261 ctgatgaatt agagcatcca ataagaaaac taaatcaaat ataatttttt ctattttatc
    10321 accatagtta tcaaataagc ttaatttgta ttcattcaat aagttagctc gtgattttgt
    10381 ttgcaattta cttgtagcct tacgttgctc ttcagaaaaa ttttctctta tataggtatt
    10441 tatggtagtt ctagaaaaaa tatttttcca tacagcaatt atctttctgt ttaacttttc
    10501 tgatgatgga atgtcattat tttcgtttat ggaatgtata atttctaaaa acttttcatc
    10561 ataataattg attatttcgc agataaaact ttccttagtt tcaaaatgct tgtaaaaagt
    10621 agctttagac attttacact cttttacaac atccgatatt gaagtatttt gaaaaccttt
    10681 ttggtcaaaa attttttgtg caatatttat aatgagcctt tgtttttctg tcactttatt
    10741 atctcctttc acgcaaaaac gtttagtact atatagtacc tacaagtttt aataaatata
    10801 ttatacatca tttggaaaaa aaatcaatat atagactgta atatgaaatt tttgaaaaat
    10861 ttgttgttat ttttagtcgt ataaaaaaga aacttaagag tttcttaaga attgtttaaa
    10921 gaagttgcaa taaattagtg gtatgattat agtatgaaag agaatgtctt gaaatttttc
    10981 tgtatcaaat tccaattatg gaggtgtagt atatgaaaaa gaaaatttgt gtagcaataa
    11041 tttttgctct atttatagtt tttgtagaaa tgtttattga tagctctagc gaaggaagta
    11101 aaattatgac agctgaatta agtaaataca ggaaaggcgt aaatgtatta tcaataaaca
    11161 atattatagt agcgaataaa aaatatagtt tgcccaaaga ttattctcct caggaaagca
    11221 gtgaagcgag agatgctttt tataaaatga ataaagatgc tcaaaagtca ggattaaatt
    11281 taaaagcttt tagtacatat agaagttatg aatatcaaga tagattattt aaatcatatg
    11341 taaaagaaca tggagaaaaa gaagcaaata gattttcagc taaacctgga gagagtgagc
    11401 atcaaactgg acttgctttt gatattggag gagatgacca gtcttgttgg gcaaataaaa
    11461 agttcaacaa tacaaaagaa gctaagtggt tgtatgaaaa tgcatataaa tatggtttta
    11521 tacttagata tccagaaggt aaagagaata taacaggata tatgtatgaa tcttggcact
    11581 atagatatgt tggaactgaa catagcaaaa actttgcaat gaataatttg actttagaag
    11641 aatatttaca tataaattaa gagatggttt gttaaaatta ggttaaaata aaaaataacc
    11701 tagactatta gctgtgtaaa gtgatataat gtaaatacaa tttaagataa ctactcgaag
    11761 gggagtagct tacacgaaag tggtaattaa tcgtcaagac agtagaaata ctcggttaat
    11821 tagcaaggga ttccttgata gcaagacctt caaaataagc attaggtatg tttgttttgg
    11881 ggtctttttt tattcaagga atttttattt aaaaaaattt aaatttcaag tatacataag
    11941 agcccccaaa cataaaataa atttattgaa gaggagggca acaaatgtct tacaaaagta
    12001 cttatcaaga agtaattaaa aatttagact caagtttatc tggtttgagt aaagaaagag
    12061 cagaagagtt gttggaaaaa aatggagcta atgaattaaa agaagcagat aaggttccta
    12121 cttataagct tttcctagaa agttttaaag acccattagt aataattcta ttgatagctg
    12181 ctttagtaca aatattttta ggtgaaacag tggagtctat aataattttt gcagttatta
    12241 ttataaactc tgttctgggt gttgtacaaa caaaaaaagc agaaagttca ttagaaagtt
    12301 tgaaaaattt atctgcacca aacgcaaagg taataagaga caataaaaaa atgacaattc
    12361 cagcaaaaaa tctagtagtt ggagatattg tattcttaga agctggcgat tacataccag
    12421 cagatggaag gcttatagaa gcacaatctc tcaaggttgt agaagggatg ttaactggag
    12481 aatcagaacc agttttaaag catacagaaa aaataagtga agatgtaagt ttaggtgacc
    12541 agaaaaatat ggtatttagt ggtgctgtag ttgtttatgg tagaggttct tttgtagtga
    12601 ctggaacagg tatggatact gagatgggta aaatagctgg gcttttagaa agtgctgaaa
    12661 ataaacttac accattgcaa caaaaaatag aggattttag taaaaaactt ggtgtaggta
    12721 ttgtaatact tgctctttta atatttataa ttcaagtagc aagaaattac tttatggctg
    12781 gaaatggaga aatgacacaa attattttag attcattcat gttctctgta gcagtagcag
    12841 tagcagctat accagaagct ctatcatcaa tagtcactat agtattggct gttggaacaa
    12901 atagtatggc aaaaaaacaa gccataataa gaaaattacc agcagtagaa actttaggtt
    12961 caacaagtgt aatatgtact gataaaacag gtactcttac acaaaataaa atgacagtag
    13021 ttgattatta tatgtatgga actaataatg atgagttaga taaagaaaaa gtaaactatt
    13081 cttatcatgc taaattattg actatggttt ctacattgtg taatgactct catataacta
    13141 gtgatggtaa agaagttggt gacccaacag aagtggcttt aataaattat tcaagtaaaa
    13201 atgatttaga ttatgataaa ttaagaagta agtatatcag aataaatgaa ataccatttg
    13261 attcagacag aaagcttatg tctactgtaa atagtatata tggagattat ataatgttta
    13321 caaaaggtgc tccagatatt gtgtttagta gatgtaaata tgctttgaaa aatggttcaa
    13381 aagtagatat aacagatgag ataatagaag aatataaaca taaaaatgaa gagttttcaa
    13441 atagagcact tagagtatta gcttttgcaa taaaagatgt cccagaagaa gattttatac
    13501 cttcaattga tgacgaacat gacatgacat tggttggaat aatggcaatg atagacccac
    13561 caagagaaga agtaatggat gcagtaaaag aggcaaagag tgctggtata aaaactgtta
    13621 tgataactgg tgaccataaa acaacagcag cagcaattgc aaaagagatt ggtataatgg
    13681 aagaaggaga cttggctctt actggaaaag agcttgatgc tttgagtgaa gaacaactat
    13741 atgaaaaact agaaaatata agtgtatatg ccagagtctc gccagaaaat aaaattcgta
    13801 tagtaaaagc atggcaacat aagaataata taacagcaat gactggtgat ggtgttaatg
    13861 atgcacctgc attaaaacaa gcagatatag gaattggtat gggaagtggt actgatgtag
    13921 caaaagatgc ttctgctatg gtattggttg atgataactt tgcaagtata gtaaatgctg
    13981 ttgaagttgg tagaacagta tacagtaata taataaaatc aattacttat ctatttgcag
    14041 gaaacttagg agctatagta tccatagtat ttgcagtgtt tgcaggttgg tcaaatccat
    14101 ttacagcttt acaacttttg tttataaact tagtaaatga ctctttacca gcaattgcat
    14161 tagggtttga aaagtcagaa aaaaatataa tgaataaacc tcctagacac ccggatgaaa
    14221 gtatactagc aggaggaaca ttgaaggcag ttgctataag aggttgtata ataggtgtag
    14281 ttacaataat tgcacaatat ataggattac aagtatctcc agaacttgga gtagcaatgg
    14341 cattttcaac attgatatta gctagaataa tgcaaacatt acctgcacgt tctaataatc
    14401 aaacagtaat aaaattaggt tttttcagca ataaatacgt tataggagca gttttagtct
    14461 gtcttgggct ttactcttta acactaatac cagtgctaag aggaatattc tctataccaa
    14521 caacgtttgg attgtaccag ttggggattt gcctagggtt agcattaatt tcatcaatat
    14581 taatggaact taacaaatta ataaatagaa gtgaagtaaa ataatcaaaa aattaaaggg
    14641 catattgatt ttaacaatat gccttttata aatagtctaa tttattctat attatcgatt
    14701 atttggaatg cttttataac ttcaggtaat tctttttctt caggcattct aaatcttatg
    14761 aaatttttat caagtccagt aaaatcactt ccagatataa ctcttatttt taatttagca
    14821 aactcttgtt gtaaatctat gtccttgttt ttatgttcaa ctgtcataat tgatacagta
    14881 tcactagttt cagctatatt aaggtttttc catggaatta gtaattgatt ttttatatct
    14941 tttgtttttt cttttaattc ttctataaat tgaacatcat ctaataaatt tgctgcaata
    15001 ctacgagata tttcagacac ttcgtaagga gtagaaatct ttttaatagg gctaacaagt
    15061 tgttctggaa gaactgcata tcctgctctt agtcctgcta aaccaaatcc ttttgaaaat
    15121 gtttttaaag caattacatt atcataatta tttagaagct tgacagctga attttctttt
    15181 ggcatgtatt caccataagc ttcatctacc ataactgcta tatcatattt agctgcttcc
    15241 tttactatat tttcaattga tgataaaggt ataatttgtc ctgtaggatt gtttggattg
    15301 tctatgtaaa ttactttata ttctggattt attttttcaa taaattcttt ttcattaaac
    15361 ttgaaattat cctctttctt taaaagaaca taatcataag ttgcaccatg catttttata
    15421 tctgtctcat attctgagaa ctgaggtgaa tatcctaaaa ctttatctcc cttttcaata
    15481 aatagtctat ttaaaaggta aataacatgt atagaaccat ctgccaaaca aattctgtca
    15541 gtatctaatg ctataaaatc tttccaataa tcgactatat tatcttttaa agcaattgaa
    15601 tgaggatatc ctcttatcat atcaaatttt aaagcattaa aagcttctac agctttgctt
    15661 gagtaagaca cggtatttat accttcacca cagtctatct cgaatacatc ttctgaatgt
    15721 ccagattcta ttgcataact ttttttcaac tgcatttcaa tagctttgtt aatttttagt
    15781 ttcatacttt atcactcctt atttagtcaa taataaatgt tataaaagtt gctagattta
    15841 aaatattatg aaaatatata atagtaaaaa atgaatataa atattataat agctcacttt
    15901 atttaatatg attatatatc atatattgta aaatttctag cattataaga ctataaattg
    15961 aaaatattca gaaaaatact acaagaaacg atttattttg ctcttaaaat aagttaaatg
    16021 aatgttatta attttgtaaa ttgaaatagt ttaagtataa agttatgata taatctaata
    16081 agggtaattc tttagattat attaaggaaa tataggtata caataaaaag ttatggagtt
    16141 ggattatggg atataaatta attgtaacag atatggatgg aactttatta ggcaataatc
    16201 ataaagttac agatgaaaat aaaactgcac tacaaaaagt tataaaaagc ggtattaatg
    16261 taactttagc aacaggaaga gccttcgatt cagctaaatg caatgtagat tttcttaaag
    16321 aggacatgcc aataatagct tgtaatggtt cattaataag agagcaaaat ggaaatataa
    16381 tatattcaaa taagattgat acaagaacat gtttaaacat tttagatgta ttagataaat
    16441 acgatattta ctaccaatgt aatagtatag attctatgct ttcgaaaaaa atagaaggac
    16501 gtgaagatag attaagtgta tttttaggct ctgaaacaga agtaattgta aaagatgatt
    16561 tgagagaaga aatctttaaa aaagatatac taaaatttgt aatcatagaa gaaaaaaatc
    16621 catctatttt ggatgaaata agaaaagaat tgagaaaagt tcaaggaata aagataactt
    16681 cttcatggcc aaacaatatt gaagtcatga atgaaggtgt tgataaaggt aatgcagtta
    16741 aaattttagc tgaaaaaatg aatatagaca gagaagatat aattgcattt ggagacaatt
    16801 acaatgacat agaaatgata aagtttgctg gtcttggggt agctatggga aatgcagaag
    16861 aattgattaa acaagaagct gattatgtta cagatacaaa tcaagacagt ggagttgcaa
    16921 aggcaattta taaattccta gagttaaaag aatcacttag ctcttaaaac agtatataat
    16981 aggaaaaaat aggttgacta ttttgtagat agtaattata atacaaatag ataaaacata
    17041 aaaatttaga ttcagtgaaa aagagagtag atatagagaa aatctcagcg aattggggga
    17101 tggtgtgagc ctaatgataa cactttatat tgaaaagaac tttggagaat actacttgaa
    17161 attcagtatg gtagtaggta atctcgcctt aagagaatag agtgggtaaa cattgtttac
    17221 caactagagt ggtaacacgg aatatttcgt ctcttggtct ttttgaccaa gagatttttt
    17281 agtttcattg agttacaaga cttataatta aaatacaaaa aattaaaata tgaggtgaaa
    17341 gttatgaaat tatggggcgg acgctttaga aaagcggaaa atcaacttat ggaagaattt
    17401 aataaatctt ttggctatga ttgtgtactg tacaaaaaag atatcgaagg aagtgtagca
    17461 catgttcata tgcaagtaaa atgtggactt ttaacagaag aagaaggaaa gtctataaca
    17521 gaaggtttaa aagggatact tgaagatgta gaaaatggaa agttagtatt ggatggagag
    17581 tatgaagata tacatagttt tactgagata aatttaattc aaagaattgg agatgttggc
    17641 aagaagcttc atacagcacg tagtagaaat gaccaagttg cagttgatat gagattgtat
    17701 gctaaagaaa aagcaaatga tttggtgggt ttaatatctg aatttaaagc tactataaaa
    17761 gatgttgctg acaaaaaccc agtgatgatg ccaggctata ctcatcttca aagagctcaa
    17821 gtagtgacat ttaagcatca cttaatggca tattatagta tgtttgatag agatgagaaa
    17881 agaataaaaa atgctataga aatattagat gaatcacctt taggctgtgg agctttagca
    17941 ggaactacgc atgatataga tagaagtata acttgtgaac aattaggatt taaaaaagta
    18001 gtagataatt ttatggatgg agtaagtgat agagattatt tactagagct aatgagtgat
    18061 ttttcaatta taatgatgca cttaagtaga ttaagtgaag agttgatact ttggagttct
    18121 caagaatttg gatttgttga gatagatgat ttatatacaa ctggaagtag tataatgcct
    18181 cagaagaaaa atccagatgg tgcagagctt ataagaggta agactggcag agtatatgga
    18241 aacttatttg gtctttttac agtaatgaaa ggaataccat tagcatataa taaagacatg
    18301 caagaagata aagaaggttt tttcgatagt gtacatactc ttgaaatgtg tatacaaata
    18361 atggatagaa tgatagccac actaaaggta aatgaggata aaatgaaaca agcagtaaaa
    18421 aatggttttt taaatgcaac agaagttgcg gattatttgg ttaaaaataa tgttgctttt
    18481 agagatgcac atggtatagt aggaagtatc gttatatatt gtgaagataa taaaaaagca
    18541 atagaagatt taacattaga agaattacat aagttttcag atgcttttaa ggaagatatt
    18601 tatgatttta tcgactatga gagtatacta aataagggaa taaaaaagaa tttaaaataa
    18661 tgtatttatt tttaaaagct gtaaatttga atgttatcat ttaaatttac agctttttat
    18721 tgtgtaagat tttattatat aaaagtaaga aacttaaaag gagtagtatt taaaataaat
    18781 acaaataaaa aattattatt aaaatgat
//
LOCUS       DAELXX010000077        18689 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.077, whole genome shotgun sequence.
ACCESSION   DAELXX010000077 DAELXX010000000
VERSION     DAELXX010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18689
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.077"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..601)
                     /locus_tag="KPH29_002539"
     CDS             complement(<1..601)
                     /locus_tag="KPH29_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1468125.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLVNQNIY
                     DLVILDLMLPNINGFDILKKMRVNNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKK"
     gene            complement(617..1453)
                     /locus_tag="KPH29_002540"
     CDS             complement(617..1453)
                     /locus_tag="KPH29_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468126.1"
                     /translation="MNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKGVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            complement(1479..2153)
                     /locus_tag="KPH29_002541"
     CDS             complement(1479..2153)
                     /locus_tag="KPH29_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG1468127.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYILKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKRKQNVKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            complement(2167..2895)
                     /locus_tag="KPH29_002542"
     CDS             complement(2167..2895)
                     /locus_tag="KPH29_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBG1468128.1"
                     /translation="MMFDRGKWNKLDYNELLEYMMSIKDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKEFLEVAEDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSGLKSMKKYKENMYDILKKYVYSKNPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKCEEETFLYIKNNNLDDFTYNKMLQKI
                     IESNRVDLEKKNIIRSMKRKTRRC"
     gene            complement(3022..3711)
                     /locus_tag="KPH29_002543"
     CDS             complement(3022..3711)
                     /locus_tag="KPH29_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG1468129.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLRVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNDFRNNILNKNANFKTPQDVKEYFDSPCIMIGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            complement(3719..4282)
                     /locus_tag="KPH29_002544"
     CDS             complement(3719..4282)
                     /locus_tag="KPH29_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG1468130.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPL
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            complement(4284..5252)
                     /locus_tag="KPH29_002545"
     CDS             complement(4284..5252)
                     /locus_tag="KPH29_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBG1468131.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLHNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFVSMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     regulatory      complement(5353..5396)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            5761..6390
                     /locus_tag="KPH29_002546"
     CDS             5761..6390
                     /locus_tag="KPH29_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG1468132.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKAIMHSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQSY"
     gene            6365..6769
                     /locus_tag="KPH29_002547"
     CDS             6365..6769
                     /locus_tag="KPH29_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBG1468133.1"
                     /translation="MKIRNKATNKKKKYVKISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            6772..7245
                     /locus_tag="KPH29_002548"
     CDS             6772..7245
                     /locus_tag="KPH29_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBG1468134.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNKIELSK
                     FDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPSS
                     LLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS"
     gene            7792..8457
                     /locus_tag="KPH29_002549"
     CDS             7792..8457
                     /locus_tag="KPH29_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBG1468135.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLKQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            8479..9411
                     /locus_tag="KPH29_002550"
     CDS             8479..9411
                     /locus_tag="KPH29_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBG1468136.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            9432..10073
                     /gene="pcp"
                     /locus_tag="KPH29_002551"
     CDS             9432..10073
                     /gene="pcp"
                     /locus_tag="KPH29_002551"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBG1468137.1"
                     /translation="MKILLTGFDPFGGELINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIK
                     GGFIHIPYTTSQVINKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(10192..10524)
                     /locus_tag="KPH29_002552"
     CDS             complement(10192..10524)
                     /locus_tag="KPH29_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1468138.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            10790..11410
                     /locus_tag="KPH29_002553"
     CDS             10790..11410
                     /locus_tag="KPH29_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG1468139.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKEAI
                     PGCISCASCFETGKCIRNDKVNELIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
     gene            complement(11735..12559)
                     /locus_tag="KPH29_002554"
     CDS             complement(11735..12559)
                     /locus_tag="KPH29_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HBG1468140.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNI
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     TLFMILVPVPFLVEGGPIWIILGIIFYLCRGKKFSLSICYVLMCIFIFTTMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
     gene            complement(12570..13973)
                     /locus_tag="KPH29_002555"
     CDS             complement(12570..13973)
                     /locus_tag="KPH29_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1468141.1"
                     /translation="MKWKITRNFIFTIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIREYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRDIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDNGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            complement(13977..14702)
                     /locus_tag="KPH29_002556"
     CDS             complement(13977..14702)
                     /locus_tag="KPH29_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1468142.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDVQNESKNLNKNEEKIISFGPYILNESRAELIKDG
                     KSIGLSAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
     gene            complement(14814..15587)
                     /locus_tag="KPH29_002557"
     CDS             complement(14814..15587)
                     /locus_tag="KPH29_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1468143.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIFLGFLLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNEPIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVRYNVPSILIVFSIVISIILMIVLTKITYENFTSSKRF"
     gene            complement(15593..16480)
                     /locus_tag="KPH29_002558"
     CDS             complement(15593..16480)
                     /locus_tag="KPH29_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1468144.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDANYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRVGIAQVLLNDPKIIVFDEP
                     TAGIDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDIDSVVLRIVSEDIPSGDNIQPVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            complement(16494..17516)
                     /locus_tag="KPH29_002559"
     CDS             complement(16494..17516)
                     /locus_tag="KPH29_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468145.1"
                     /translation="MVNWEIKKLAKSKSMFISLGILVLILMISIFITPVLETERYYID
                     KEKGCIEDTRSGIDIGNEKFQNKINVLKQFSNQTDTGEFSKKIELMSKKKLDNLSVNE
                     YKDISFWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALK
                     SKIFVALLVPLVIYSVYLMGIFIITCMQQGVPVNGDLESFRIVDNVVALKTNLSITKY
                     VILNIGIELLMFEGWAMIAIFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLS
                     VVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGAMSLCFLVIK
                     SDYISR"
     gene            18091..18267
                     /locus_tag="KPH29_002560"
     CDS             18091..18267
                     /locus_tag="KPH29_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468146.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     TCLHQVLLLVSRYN"
BASE COUNT         6573 a         2901 c         2016 g         7199 t
ORIGIN      
        1 gcttttttct tagattactt acatatactt ccactatttc aatagttgca tctgaatcaa
       61 ttccccatat tctatcataa atctgctctt ttaataaaat tgaaccttta tttaatacaa
      121 aatactcaag caagttaaac tgtttgtttt gaagttttat ttcttctttt tcaatataca
      181 ctcttttttc tagtgtatct aaatataaag atttaaattc taatccgttt ctttctttta
      241 tctttccatt agtccttaat atagcgtata ccctagctac taactcttcc atataaaaag
      301 gttttgtcaa ataatcatta gctcctatag taaatgcttc tactttgtca tctaatgttt
      361 cttttgctgt caatataagt acaggtgtat ctatattatt aactctcatt ttctttagga
      421 tatcaaatcc atttatatta ggaagcatta agtctaaaat aactaaatca tatatatttt
      481 gatttactaa gtataaagct tcttctccat cttcacactt ttcagtttca aaatgcttgc
      541 taagttcttc tactacactt tctaaaagta tcttattatc ttcaactatt aaaaccttca
      601 taagtatccc ctacctttat ttgtctatct taactttatg agattttaag tcaatatcca
      661 cattggcttt ttcttttata tcatcatacg tatatcccac tatttcaaca ctttttgcct
      721 ttgttgaaca caatatatct atatctgtct cttctgaagg ctttaaaatc ttatatgatg
      781 gtacacgatt tactgttaaa tattctccat tttcattaac ttctgctgtc ttaagaataa
      841 tatttcctaa atcttttgat gatgaatttt ttacttttac ttgatacgta attccctcat
      901 ttgattcatt tactttttta agttctgatg tagatagcac ctcatactga ctactatttt
      961 ctagcttaat attattattt cttatattaa tagtattttc ttttaaatta acatacacaa
     1021 tcttaccctc tgcctcatat tcatatgcat aaacttctac accctttaca aaagttttat
     1081 gcccacattc tatgtaagca actttcccag gcattaaagt catatccaac aaagccttac
     1141 tatccgaaga tataattttt ttattctcat catattcatt atatatcaat tcaacattgg
     1201 atatattaaa tgttgaatta ttttctacct ttgttgatgt atctacttta tcaccttcat
     1261 ctatatttat aatttctgat tttcctatat tcaatacttt tgcatttccc tcatctattt
     1321 ttatctcatt tttatcttct gtttgaatat caactgaatc aggctgttca tctttttcac
     1381 atgctgtcaa cataaaaata aatattgtta atataatcat tagagaaatt gattttttta
     1441 ctattttatt catattaagc acctcatata gtagtatttt atagtagatt tttatacttt
     1501 ttcataaatt ctttcaatgc ctgtgtaagt atttgatgtt tttcaaaatt cgaattacta
     1561 cataatcttt caaaatcatt ccatatttct ttatctatac gtattcttgt tgtttttaat
     1621 tcatctatgt gttcatcatc tatatgtaca tcttttttat tagatacttt tttaggcttt
     1681 atattgtaat cttttacttc taaataccaa tcatatactt cacaaagctt atctaaatct
     1741 tcttgagtta tatttatttt tctatctttc ttaggcttaa cattttgttt tcgctttgca
     1801 ttttttaaac ctgtatcatt aaactcaatt tgttcaacac ttttacttaa agccttttca
     1861 ttagaaatct tttttttggt gcttactttt tttctattac ttatagtatt ctttttactt
     1921 ttttcatact ccttagcttt ctcttcttcc aagtttctat tatccaaact tttattataa
     1981 tttaattcgt cttttaatat gtatttacct ttttctgact tatatccttt tttatttaaa
     2041 aaagttctta aagtactttg actaatcttt agattttgag ctatctctgc aaatttaaat
     2101 cctttgcttt gaagttctaa aaatttgtca attctgtctt cattgttatt cattaatatt
     2161 cctccgtcaa catcttcttg tctttctttt catacttctt attatattct tcttttctaa
     2221 atctactcta tttgattcaa ttattttttg tagcatttta ttataagtga aatcatctaa
     2281 attattattt tttatataga gaaaagtttc ctcttcacat ttaataaaac aaattgatag
     2341 caaccatgcc atacccattt taacataata ttcttcactt tgtatattat tacagtattc
     2401 aaatatttct ttaatatgtt tatcatcaac ataatatatt aaaaacataa ctaatgcaaa
     2461 tctaatttcc catggattct ttgaataaac atatttttta agtatatcat acatattttc
     2521 tttatatttt ttcatagact ttaaaccaga acaaaaccca tcgcaaactg accaattatc
     2581 gattttaggt ataaatcttt ttacatagta taaagtttct tcaaaattac tatttatatt
     2641 gccaattacc ataccttgta gcctaacttc ttcatagtat gtatcctctg ctacttctaa
     2701 aaattccttc caattccctt ggcttatttc ttttgataac tttcttaaat taggaactct
     2761 aatacctatt atattttctg ttccaggtat aagcttttta ttaaaatctc tatattttat
     2821 atcttttata gacatcatat attctaaaag ttcattataa tccaatttat tccattttcc
     2881 cctatcaaac atcatttaat tgctccttaa ttcctttttt cttattgtaa cataaaactc
     2941 tacaaaaaaa gcttctaact attagaagct ttttcaaaaa cataagaaac ttttcaattt
     3001 taaatttaaa ctaaattaat gctaatctcc tacaccaaag tctgtattat tgtataaaac
     3061 ttctatattt ggattttcat ccaagaattt atacttacta acaaaccatt tcactaactc
     3121 ttcatctctt tttatactag aagaattatt tataaaaaca ttcactgtcg ctttttcaaa
     3181 agtatttagg ataatctcta tatctttatc tatcatctct tttgtttgtc cttttattcc
     3241 aatcataata caaggtgaat caaaatattc ttttacatct tgtggagtct taaaatttgc
     3301 atttttattt aaaatattat ttctaaaatc attgtcaaag gtttcaactc caattttaaa
     3361 taccacagga atttcaaaat actctctcat ttccttaagc ctatttttgt aagaccaatg
     3421 actttctaaa aataattttt ttatattctt ctcttttata atacacttta ttttttctaa
     3481 agtatcctta ggtaattcaa aacagcttcc agaatttatg acttcaagaa ctccatattt
     3541 cccagttaca ttttttaaaa cccttagatt taatttattc atctcttctt ctaaattaga
     3601 attatcatct atataatcac aaaaagaaca ctttccccat atacaaggaa atcctttaag
     3661 cagaacaatt tctctttgat ttttatttgt tatctcacta tatctatcca ttttctatct
     3721 attttcctcc agtcaatgat acctttaaag tttttggaac tgcatttaat cctaaattca
     3781 ctaaaacaac tgctaacagc ttatctccat aatcagtaaa tacttgggtt acaaacacac
     3841 taaaaacatc tcccagtcct aacactttca agatttgcac tatataggat gaacctgaag
     3901 aggtcatacc tccaaataaa aatgctgaaa ttattgaact tattatagat gttggaatag
     3961 caaataaaag tactcctagt ggagttttta aaccccttaa aaaattcttt ttaaatgcta
     4021 ttccagcaag taatccggtt gatatttgaa ctggtgcaaa atagagagaa tacacatcaa
     4081 acgtaattcc acttattaaa cttccaaaca cacctgtaac aacagcgtat ttaggtccta
     4141 aaagtgaagc tatcattata gttcctattg aatctaagta tacaggtaat cttaatccca
     4201 tagctataaa agcgcctatt atatttatag aaataccaaa agctataagt gtaatagttt
     4261 ttacacatat ttttcttttc acactatatc actccttcca cagagtctat aatgtcttca
     4321 taacctttaa aaagcctctt taaaaacatg tacataaatt tcttctcatc aacttcattt
     4381 agaacaattg cattaggatt ttttttatag aaattgaaag aatccactat agactgaccc
     4441 atagctattc catcttctac aacttctaca tatgattcaa atcctttaca tatacttctg
     4501 tctataaagt acgctactgc tagagggtca tttatcacac agccaattat tccttcctgt
     4561 tcccaatgga aatcaatata aaatctagtt atttcagtta tatactttgc catcttctta
     4621 tcaagtctat ttataaactc aataatatta ggagtaagta caatttttct agttacatct
     4681 aaacctacca tgtggatttt tttagataaa ttcttgtaaa cataatctgc tccatgtggg
     4741 tctacccaat aattaaactc tgctactgga gagcaatttc catgaatcct aaatgctcct
     4801 cccatagata caaactcatc gagattttca aatgctttct tatctttaat aagtgcttta
     4861 gctatatttg taagtggtgc aagtgctatt attgatactt tttcatgatt atgtaaagta
     4921 tttattataa aatccactgc tccatttttt gctttagctc ctacaacctt ttgataaaag
     4981 ttttctccaa taccatcttc tccatgtgta tcttgagctg ttacaagttt tctttttagt
     5041 ggtgcttctt ctcctatata tactggaata tttagtgaag aacacatttg aagtgttttt
     5101 agtgcatttt ctgcacctat atttgctgga acatttccac aacatgtggt aattccaatt
     5161 acttctagct ctggtgagtt taaagccaga agaattgcca aagaatcatc aattcctggg
     5221 tcacaatcaa ttattacttt tctcttttcc attttttatc ctccttgatt tgtggtcgag
     5281 gagaactaca catctttttt ccactttcaa aaaggtgtgt ctaagggtat taattatata
     5341 ctgccactat ataattcctt agttttttat acttggagtt ctcgaacaag tacagatgta
     5401 aaatcatatt tttaggctac ttactataga ctcttaaaaa ctatagactg ttaaaaaatt
     5461 tttaaacatc tgatttaata gctataaaat aaattttata gcataaaaga atttattata
     5521 ttttaatgta tcttattaaa gtttatttag aattatgaac caactttaat aagatgtggc
     5581 gcatttaaac cgattattat tatacaataa tatcttaaat attacaatta ataacattaa
     5641 tttattatcc ataaaaatat attaacttat attatttaat aatttattga cagtattaaa
     5701 attaaattat acaataacag tgtaaattaa cttaacaccg ttaagcaaag aggtgattat
     5761 atgagtatca aagaaagacg taaaaatgaa aaagaagaaa tgaagaaaaa gattatggat
     5821 gctagcatcg aaattataaa ccaacatgga tatgaaaatt tatcaataag aaaaattgct
     5881 acaaaaatag aatattctcc aactacaatt tatctttact acaaggacaa agcagagatt
     5941 atcagcgata tgaccaataa actttataac acagtagaaa gtaatgctat tgacattatg
     6001 aataattgct catcactacc aatagataaa caaatcatag aaattatgac tagctttata
     6061 aaaactcttt ctagtgaacc agagatggca aaagctatca tgcatagtag aatgaatatt
     6121 atatttgcat ctgaaaatac aaacaatact cctagcaata atggtataaa aatgcttgat
     6181 aaattccttt ccattggaat tgaacaagga atattcaaaa aaaatataga taaaagctca
     6241 tggatgctta taagtgcatt gttaggattc gttttatgtg ttgttgaaaa taaactatat
     6301 tctttacata atttttctca acttatagaa aatttcttag atattttagt gggaggactt
     6361 tacaatgaaa attcgcaaca aagctactaa taaaaagaaa aaatatgtaa aaatatctat
     6421 tgctctattc tttgtattta tatgcattat tattgtaatt gtaagcaact taaagccaga
     6481 aaacttggta gtaaaagata tagatattaa taatgttaaa aatggcattt atactggaag
     6541 tgctgataat aacttggtaa aagctactgt gtctgttgaa gttaataatg gtaaaatcca
     6601 aaatattaat atcttaaaac acgaccatct tttaggtaaa cctgctgaaa aaattacaac
     6661 aagtattatt aaacaacagt ctttagatgt ggatgctata acttctgcaa cttatagcag
     6721 taatactata cgaaaagctg tagaaaatgc tttaagaaag ggagaataat tatgaatact
     6781 attattatat attcaagtaa atatggttgt acaaaagact gtgcaaatat actaaaaaat
     6841 aaactatcag ataatgtcac tattgttgat attaataata ataataaaat tgagttatca
     6901 aaatttgata aaattattat tggaagttct atatatgttg gttcagtttc aaaaaaaata
     6961 caagtgctat gtaatgataa tgtagagtta ttaaacaaaa aacaagttgg cattttcctt
     7021 tgctgtggct tttctgaaca agcagataaa tatttaaaat caaattttcc atcatcctta
     7081 ttagaaagtg caaatgctat tggaattttt ggtagtgaag ctcgattaga aaaaatgaaa
     7141 tttttagata aacttattat gaaggctgtt tcaaaaggta attatgacag ttttagaata
     7201 tcacaggata atatagataa ttttttaata aatcttaata gctaaaataa aactaattat
     7261 ttctatgata aattctttta tttataagta atattgctca aaatagtctt taaactttat
     7321 caaaatttta tttttcttag tgaaatctaa tgaaactagc tcttaatata tgctttatta
     7381 atcataacaa aaagagagtg tctaatgaac taaaaagttc attagacact ctctttttgt
     7441 atgaaatcaa atatattttt atcatttcaa aagagaaaaa tacacttgtc tctatgtttg
     7501 acacattctc tttttataat ataatatcta atttaaaacc tatttcaaat attgacatat
     7561 acttaaacca tagacttaca aaatcaattc atattagctt aaaatataat ctttgttact
     7621 caaattccta tacacaacca ttatttactt tgatacttta aaacatcttc cctatttttt
     7681 attaaatttt ataataatag tcctattaac gcaaatacta taaaaagagt ttaactttat
     7741 ccattaaata aatttaaatt aaaacatata gaaatggaag gagaacaatt tatgataaaa
     7801 ctaatcggta tattgatagt tgtaattgga tttatactta aaattgacac attatttact
     7861 gtattattag caggagtggc aacaggaatt gttgcaggat tagattttaa tcaaattttt
     7921 acaattttag gagattcttt tgtctcaaac agaggtgtat cattattcat attaacactg
     7981 ccagtaatcg gtgtattaga aagatatggt ttaaaacaaa gagctgtttc tttaatagaa
     8041 aaattaaaaa gacttacaac aggaaaagta cttactatat acatgatagc tagacaaata
     8101 gctggtgctc tatcaataag aatgagtgga catcctcaat ttgtaagacc acttgttaat
     8161 cctatggcac aagctgctgg tttaagtaat agtgatgaac taaaagaatc ggatgaagaa
     8221 gccataaagg cattatctgc tgcatcagaa aactatggaa atttttatgg tcaaaactta
     8281 tttgcaggta gtagtggagt attacttata gcatctactc taactcaatt tggatacaat
     8341 gtaactggac ttaatattgt aaaggctagt actattatgg cagtaatagc acttgtagtg
     8401 gctacaatcc agtttacctt atatgataaa aaattaaaca aaagtcataa aaaatagttt
     8461 tatttaggag gaataaaaat gcaaaatttt ataaacatat cattggaaat cttttacgca
     8521 ctaatgggat ttttaatgat tgttatagca tataagtctt ttactaccat aaataacaat
     8581 aagaaatatg gtacatcatt attttggata cttatatcac tgccattcat atttggtaga
     8641 ttgattccag ccaatataat aggaataata ttaatattat ctagtttatt aacactttct
     8701 aaacaagttg tatttgcaaa gtatgaagaa ccagatgaaa attttggaaa agaacaagca
     8761 gacaaactta aaaataaaat atttatacca tcattaatat tagcatttgc tgcagtagta
     8821 gtagctatga gcctttctaa ttttgataat tcttctcaat ttgcaatagg tgtgggttca
     8881 ataattgcat taatatcagc ccttataata actaaagcaa aacctgctac atcagttcaa
     8941 gatggctcaa gattacttca acaaatggga cctgctagta tgttaccaca acttttagtt
     9001 gcattaggag ctttatttac tcaggctgga gtcggcgaag ttatttcaac catgatatct
     9061 ggtgtagtcc ctgctgatag cagattattt ggtgttatag cttatgtatt aggtatggtt
     9121 atatttacca tgataatggg aaatgcattt gccgcatttt cagtcataac ggcaggaatt
     9181 ggtattcctt ttgtattgtc tcaaggtgga aatcctgcta taattggagc attagcatta
     9241 acagcaggat attgtggaac tctacttact ccaatggctg ctaattttaa tatagttcca
     9301 gcagccttgc tagaatgtaa aaatgattat attgttataa aatatcaagc tccagttgct
     9361 ttggtattaa ttatagccca catattagta atgtactttt taggatttta atataattag
     9421 gaggtaaaat aatgaaaatt ttgttaacag gatttgaccc atttggggga gaactaataa
     9481 acccagctca agaagcagtt gaaagggtca ataataatat aaacggtgca gaaatcataa
     9541 agataactat accaacagtt atgacaaaat ctgttgaagc tatagataaa gctattcaag
     9601 agcacaatcc agatatagta atatcagttg gacaagctgg aggtagattt gacattacac
     9661 ctgagagagt tgctataaat atagatgatt ttagaataaa agataatgaa ggtaaccagg
     9721 ttatagatac tattataaaa gaagatggtg aacctgcata tttttcaaaa ttacctgtaa
     9781 aagccatggt aaaacatatg aatgaaaata aaattccagc tagtgtttca aacactgcag
     9841 gaacattcgt ttgtaatcat gtgatgtatg gaatccttta tatgatagac aagaaatatc
     9901 ctaatataaa gggtggattt atacacatcc catatacaac ttcacaggta ataaataaaa
     9961 aaaatacacc attcatgtct ttagaagaaa tagtaaaagg tttagaatta gctatagaag
    10021 cttgtattat atacaaagaa gatgtaaaag aaattggtgg tgaaatttca taataaattt
    10081 tttagtattt tataaattaa aaatactaac tattaattta aatatagact gtgagtagat
    10141 tattcaaaca attttactca cagtcttttt atggcatcta cagatacaaa attaaggtct
    10201 attcctctct ccccactcac acattgcatc taaaattgga atcagagatt ttcccctttc
    10261 tgataaacta tattctacct taggaggtat ttgaggatat tcagtacgaa taatcaagtt
    10321 atctgcttct aattctttta atgataaact caaagtttta taggaaatag tatttatata
    10381 acgttttaat gcattatatc tgacaacttc aaattctaca agacagtaaa gaattatcat
    10441 tttatattta ccactaatca gtgaaagtgt ataactaaat cctgtttcac ttaaatctgt
    10501 atttgatata cagtctatac tcatatatta cactctcctt ttggttagta ttgaacaaaa
    10561 atgtgcgtac ttgtttttag aataatttat aataaaatta tagtgtaaga ataattgaaa
    10621 gtcaatttta taataggagg tgtatttact aaaaaaagag ttattttaaa taggattcta
    10681 agattagaca tttctatata aattgataat attcatgaaa atcctatttt ataaaaaata
    10741 atatctctta gaaaatctat taaataaaat tcattggagg aaatttataa tgaaagtttt
    10801 attgataaac ggtagcccaa accaatatgg atgtacttat acagcactaa atgagattac
    10861 taaagtattg agtaaacacg atattaaaac agaaatactg tatttaggta aagaagcaat
    10921 cccaggatgt attagttgcg catcctgttt tgaaactgga aaatgtattc gaaatgataa
    10981 agttaatgaa ttgattgaag atctcgataa tattgatggt attattattg gttctcctgt
    11041 ttacttttct agtgcaactg gtcaattaac ttcttttctt gaccgtctat ttttcatagc
    11101 tggaagtcgc atggctacaa aattaggtgc ttcagttgtg tcatgtagac gtggtggtgc
    11161 aagtgcaaca tttgaccaac taaataaata tttttcaatc agcaatatgc ctattgtatc
    11221 atcacagtat tggaatcagg tacatggctt tactccagag gatgttatga aagatgaaga
    11281 aggattacag actatgcgaa ctctaggaga aaacatggca tggcttctta agtgtatatc
    11341 agcaggtaaa aaagctggaa ttaaagaacc acaatatgaa gaaacaatta tgactaattt
    11401 tataaaataa ataaaaaatg cactaatatt aattaaaaca aaacactaat ttataaataa
    11461 aataagtata aatataggat aaaaaacact aaaacagagg tattgaattt tatatcaaac
    11521 ctctatttta ttattacatt aataaccaaa ccatatataa catcatatat ttctaaataa
    11581 attataatat tatattctaa tgttattgaa attacaataa ctgaatttct gctcataaat
    11641 ttaaatattc tacaaatctc atctaaataa caattttaat agacctaaaa caatcctcat
    11701 attgttcatt ttgtattaaa aatatcactt acaatcaaaa accatttatt aagtatattc
    11761 ctaagatata caatatatat acatggattg gatagtataa ataaaataaa tacttcatac
    11821 tcttcccttt ttctccattg taaagtaaca ttaaaggtag agaagcaatc atcatccatt
    11881 ggtaattttg taaaatagaa ttttttaaac tataatctcc atttgacatt gttgtaaata
    11941 taaatataca catcaaaaca taacaaatac tcaaactgaa ctttttacct ctacacaaat
    12001 aaaaaataat cccaagtata atccaaattg gtccaccttc tactaaaaat ggtactggta
    12061 ccaaaatcat aaataaagta ataactctgt tagtgacttt aaaaagtaaa gctactgtaa
    12121 atattcctaa aattatagga atgattatca tcaataatcc tataataaaa tatttaattt
    12181 ctttctcttt tctaaatttc tttatgaact ctattgcttg taaataaatc gctattaaaa
    12241 ataatgttga aaaaatgtta tttataatta tagctccttc tggatgtgaa aatacattat
    12301 taattatttg atttcctata gccataataa cagaccaaat atacaatcta cttatatatt
    12361 tttttctgtt actagtgtga taaaatcctt caacagccat gaatataaac agtggtgctg
    12421 atattctgcc tacccaatga aaccaaattg gtatttgcat tgtttgtggc ataaatgctg
    12481 ctatatggtc aaatgtcata aatattaatg ctaaaatctt aatagtaaac ccacttatac
    12541 cttttttaaa aaaatccata tcatatctcc taattttgtt ttatctctat aataattttt
    12601 gttcctattc caatttcact acttacataa attttacctt tatgaatctc tattatttct
    12661 ttagaaatag ccataccaag acctgaacct ttatgcatct cacctgtatt agtacctcta
    12721 taatattttt taaaaatatg tttcaaatca cttttgctta ttccaattcc attatctttt
    12781 attataattt ctatattatc tttttttaca atttctacac ttatcaaagt tccttcgcta
    12841 ttatgaacta ttgaattaaa tataagattg gttatagccc ttctgaataa gattgaatct
    12901 acatatactt caattaaatc ttcattactt tcaaattcaa tattcctatt tttatatcta
    12961 ttatcattta aaatatcaat tattatatct ctgactaaac tgactaaatt tatttttttc
    13021 ttgtccagaa ctatagtatt attttttaat cgagtagata aatttaaatc atcaactaaa
    13081 tcttttatat acttagactt attatatata atttctgtat actctcgtat ttcatcttct
    13141 tcaaactcat aatctttgtc attaattatc tctgcatacc cttgtataga tgctagaggt
    13201 gtctttatat catgtgttat atttgccagc cattcctctc tcaactcttc attttctttt
    13261 ctctcaactt catttactct taaagtatca gctaacatat ttatattttt aaaaacttct
    13321 tcatatatac ctctgtcctt aagatataaa ctataatttc cattagccat agtctcaata
    13381 ctccatagta cgcttgaaat tggttttgta agttttctac taaataaata tcctacacct
    13441 aaagctacca cgctatcaat tactagtgtt ataatgataa ctttatttaa agttttttta
    13501 atgttatttt gagaataggt taaaattatt cttgatatat ctctactagg tatacctact
    13561 attatgttta gatgtttttt gtttaaatat ttttctagta caaaattaac atactttgtt
    13621 tctatatact tgtaattatt caccatttga aagggtgtat atttttttgg agtatccttt
    13681 ggcacattta ctccataaac ttcctctcct aaatcattta aaacctgtat ccaggaattt
    13741 gatttttcta atttttcagc tccaatctta gatagtttaa attctccatc tttttcatat
    13801 aaatcttttt caaaagaacg tgcaaattct tcaggattat ttccagaatc tacaacttta
    13861 aaaaagctat tagttgaaat cacatacaat atagatataa tatttataat tactactgat
    13921 atagctacaa acacaattgt aaatataaaa tttctagtaa tcttccactt catattctaa
    13981 tcttccttta ctgctaattt atatccaaga ccttttattg tcagaatata ttcaggcttt
    14041 gaaggattat cctctaattt ttccctaatt tttcttatat gaaccattat agtattgtca
    14101 aatccaacat aatcttctcc ccacacttta tcccatagtt tttctttact tataatttga
    14161 ttttgattat gtgcaaataa agataggatt tttagttcct ttgcagacag tcctatagat
    14221 tttccatctt ttataagttc tgccctactt tcatttaata tatatgggcc aaatgatata
    14281 attttttctt cattcttatt tagattttta ctttcgtttt gaacatcact aaaataactt
    14341 aatcttctca aatgcgcttt aactctaaaa gccacttcct tagggctaaa aggttttgta
    14401 acataatcat ctcctccaat cgctagtcca agtaacttat ctaattcttc ggttttagct
    14461 gataaaaata ttattggaga attggaggtt tttcttattt ctttacaaat atcataacct
    14521 tcgccatctg gtaacataat atccaataca atcaaatcag gattaatact tttaaataat
    14581 tctaatcctt cttttttagt ttttgcagta tatatattat taaggccctc tttttttagg
    14641 acagtttcta aaagcttcaa tatttctact tcatcatcta ttattaaaac acgattttcc
    14701 ataaacaacc tcctgtaata atttacatat atattagcat atattttatc taaaattcat
    14761 ttatcttatt tttaattaaa ctaaaatata aataaaatta ctattatttt tagttaaaac
    14821 cttttgcttg atgtaaaatt ttcatatgtt attttagtta acactatcat taatataatt
    14881 gagattacta tagaaaaaac tatcaaaata cttggtacat tatacctaac catttgttgg
    14941 ctcatcataa tccaatttgg agtcactgac ataaatttta tagctttatc tggtataaaa
    15001 cctacactaa agtatggaat tgccattatc aatccaccta gtataaatgg aattacagcc
    15061 gatttaaata tagatgaaag tgttaagcct atttgtgcaa atataaaaga agcaaaaata
    15121 gtagtcaata taacaattat atacatttgt attactgtaa gatttaaatt actatttcca
    15181 aagtaccata agttatgaat cggttcattt aaatttttaa aatcaaaaaa actaaaacct
    15241 ctaattccta ctaacaatat aaaagaaata ttaataatag ataaaattaa tccattagct
    15301 actagttttg ataatactac tgattttttt ccctttttag ttgtatttat taaacctgtc
    15361 atattagaat catattcttt aatgtaattt ccagaagtta ttataaacat tatcaaacct
    15421 attagaggta ttgagccttc tatcttgtaa aatatatctg cataagtatc taaaacatca
    15481 aactttctaa tcagaagaaa tcccaaaaat attaaaaaca gccaaattat ccaaacactc
    15541 tttgatttta atatttttat catttcagct ttaactaaat acatcatttc tactacacct
    15601 cccttgcatc ttccaaatca aaataaaaca tgtatacatc ttcgaatttt gcttgaactg
    15661 gttgtatatt atccccactt ggaatatcct ctgaaacaat tctaagtact actgaatcaa
    15721 tatcactttg aatacttaca accttatatt tagattgtat atcatcaact tcacattcat
    15781 ttttaacttg aacattatat actttatttt ccatgtgact taatatgtct ttgtgtgttc
    15841 ctttcattag aagtttgcca tctttaatca ttattgtttc ctttgcaact gattctatat
    15901 cggaaattat atgagtagaa agtattatta tcttatctct agacattttt gctattaaat
    15961 tcctaaatct agctctttct tgtgggtcaa tacctgctgt tggttcatca aagactatta
    16021 tcttagggtc gtttagtaaa acttgtgcta tcccaactct cctcttcata cctcctgaaa
    16081 attttcctat agacttattt ctcacatcgt aaagacctac taaatctaat aattctccta
    16141 tcttactatt taaatatttt ccccttaaac cttttaaagc tccaatatac tgtaaaaatt
    16201 cttttgctgt aaaattttta taaactccaa attcttgtgg caaatatcct aaaatatttc
    16261 tataattagc atccatttta aatatatcct caccattata aagtatctga ccttcatcag
    16321 gttttatcaa cgttgttatt tgtttcatta atgtagattt tcctgctcca tttggtccta
    16381 gaagaccata aactccattt tccagagtta ctgtaatctt gtcgtttgct acttttttac
    16441 catatatctt tgaaagattt tctatttcta atttattcat cttaactccc cttttatcta
    16501 cttatataat ctgattttat tactaaaaaa cataatgaca tagcacctat caatattgcc
    16561 atcaaaagtc ctattgctat attgacaatg tctatattca ctttaaatat cataatatca
    16621 cctagataat ttcctattat tttattaaaa ccaaatatca aatcataata atttacattt
    16681 gatacaactg aaagtaattt tttaggtaat atatggatta ccgacattat cttagttata
    16741 gatataataa atataaaaaa acttattgaa gatattgaag atttacttat aaaagaccca
    16801 aatatggcta tcatggccca tccttcaaac atcaaaagct ctatgcctat atttaatatt
    16861 acatactttg ttatacttaa attagttttc aatgcaacta cattgtctac tattctaaaa
    16921 gattctaagt ctccattaac aggtactcct tgttgcatac aagttataat aaatattccc
    16981 attaaataaa cagaataaat tactaatgga actaataaag caacaaatat tttagacttt
    17041 aatgcttttt tcttctcttt tgatgatagt atgatatctt ttaccgaagt aacgacttca
    17101 tctacgtaaa tattggatat aattatagat ataatgatta acattcctac atttataagt
    17161 gaatttgtaa ctctatagtt aaatactttc caaaaactaa tatccttata ttcattcaca
    17221 cttagattat caagtttttt ctttgacatt aattcaattt ttttagaaaa ttcacctgta
    17281 tctgtttgat ttgaaaactg ctttaaaaca ttaatcttat tttgaaattt ctcattacct
    17341 atgtcgatac cacttctcgt atcttctata catccttttt ctttatctat ataataacgt
    17401 tctgtttcta aaacaggcgt aataaagata ctaatcatta gtattaacac taatatccct
    17461 agagatataa acatactctt agatttagcc aattttttta tttcccaatt taccaatata
    17521 tttctcctcc taaatcattt taattacaca tatatattaa aacttaaatc ttatataaat
    17581 ataatactaa ccttaaataa agcttaaatt attttataat ttataggtgt gtttattatt
    17641 ggagttacat ttattatttt tctgggaaat tatacatttt caattatttt tattattttt
    17701 gatattttta ttgattggaa attttattgt tgttcaaatt gtagttattc tttaaattta
    17761 aagatggagt tttttaagaa tagaacctac tgtccttatt atggaaagat tattaaatag
    17821 ttgcatataa ctttatttat cttgaatctt atttttaaaa acaattataa ttttagaata
    17881 ttttctattc tcaaacaagc atgttgaata atgaagcatc ttctagtatt atatacttgt
    17941 agaataaaac taaattggta aaactgggaa tatttaacct taaggagtta agttatagaa
    18001 actagtacta aaaaatttta taaattatgt taattgacta atagaatgcg actttttatt
    18061 tttactaaat tttttagggg gatggataat atgtttattg gagaaataga caaatgtaca
    18121 catatactaa ctgcttacat cagtagttca tatgattatt gtaatttttt agatacacag
    18181 ttagatgatt ttatatcaga atatggagaa acggtagttg aaacctgctt acatcaagtc
    18241 ttattattag taagtagata caattaaaat taaatttttc tagaaaaacc tttttagatt
    18301 tatctactct aataaatata taattttttg tactttataa gtaactataa cttctgctaa
    18361 agtaaagatg tattgggagt acatctttac tttcccatta tagaagttaa aagcatacac
    18421 cataaaatca atcatccaat acaaattata tatttcctat gtctaactta atttacaaga
    18481 atcattctat atgcatattc tgcaatccac taaacatgct ctaaaccttt actattttat
    18541 acattctcta taaatcatac aaatcatata taaaatcccc ctaatatgtt taaaccacct
    18601 ataaaataac accaatattt agaatattca atatcaactg cataactggt caataaaaac
    18661 gtataactag acaaatttca atatttata
//
LOCUS       DAELXX010000078        18620 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.078, whole genome shotgun sequence.
ACCESSION   DAELXX010000078 DAELXX010000000
VERSION     DAELXX010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18620
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.078"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..360)
                     /locus_tag="KPH29_002561"
     CDS             complement(<1..360)
                     /locus_tag="KPH29_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG1468147.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKE"
     gene            928..1290
                     /locus_tag="KPH29_002562"
     CDS             928..1290
                     /locus_tag="KPH29_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468148.1"
                     /translation="MKIRKSGFISIECIISIAILYVAVYLVSTSLYNCYSFISRNISD
                     REMLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSRIEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            1274..1990
                     /locus_tag="KPH29_002563"
     CDS             1274..1990
                     /locus_tag="KPH29_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468149.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDVSNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKAIKVRVKIKNPWIGIDIEDYMDK
                     SNNNKIDDKTFNNYKASENNEILNQFKNESENIRNQEVVEFTEEDEEYEEELKQNIIE
                     NIKKNFDEKKLVQVYKYRGV"
     gene            2010..2429
                     /locus_tag="KPH29_002564"
     CDS             2010..2429
                     /locus_tag="KPH29_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468150.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEIYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            2419..2943
                     /locus_tag="KPH29_002565"
     CDS             2419..2943
                     /locus_tag="KPH29_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468151.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIMNVSNYEETFKKTTSIKCRYVDENNNEESISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVLITNNGQYVNIKLKLSKRSQKYE
                     TDFKIKVWNFSESI"
     gene            3255..3812
                     /gene="efp"
                     /locus_tag="KPH29_002566"
     CDS             3255..3812
                     /gene="efp"
                     /locus_tag="KPH29_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBG1468152.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            3904..4359
                     /locus_tag="KPH29_002567"
     CDS             3904..4359
                     /locus_tag="KPH29_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG1468153.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCPSCDDRE"
     gene            4522..5232
                     /locus_tag="KPH29_002568"
     CDS             4522..5232
                     /locus_tag="KPH29_002568"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBG1468154.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            5286..6356
                     /locus_tag="KPH29_002569"
     CDS             5286..6356
                     /locus_tag="KPH29_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG1468155.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            6384..9938
                     /gene="smc"
                     /locus_tag="KPH29_002570"
     CDS             6384..9938
                     /gene="smc"
                     /locus_tag="KPH29_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBG1468156.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKILEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEINDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLEASKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNIRDENINSEITELNKNIEIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSINLQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKSAINYL
                     KQNNLGRVTFLPLNIIKSNKVSLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     KISNIKNEISHLELKRESLDKDVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEI
                     ESLKGSITKLENEKNDLNSNLNYTLEKSDDVRKDMEELDDLYNKNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEAYNSIVRDIKRISGENCELEEKNKQLEESLNYEE
                     HEIIKLQDSIATEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESL
                     KREIRSLGNINIGSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            9965..11245
                     /gene="ftsY"
                     /locus_tag="KPH29_002571"
     CDS             9965..11245
                     /gene="ftsY"
                     /locus_tag="KPH29_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBG1468157.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDSLENLEETIFSG
                     LEEEVIDKVEDIEEKTEESVNNEGTEEIENFEKIEVEMDSNAKEVENISNNDSQEEII
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPAPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            11348..11716
                     /locus_tag="KPH29_002572"
     CDS             11348..11716
                     /locus_tag="KPH29_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBG1468158.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            11713..13065
                     /gene="ffh"
                     /locus_tag="KPH29_002573"
     CDS             11713..13065
                     /gene="ffh"
                     /locus_tag="KPH29_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBG1468159.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPVNIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMAELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            13103..13375
                     /gene="rpsP"
                     /locus_tag="KPH29_002574"
     CDS             13103..13375
                     /gene="rpsP"
                     /locus_tag="KPH29_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBG1468160.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            13440..13667
                     /locus_tag="KPH29_002575"
     CDS             13440..13667
                     /locus_tag="KPH29_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBG1468161.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            13775..14290
                     /gene="rimM"
                     /locus_tag="KPH29_002576"
     CDS             13775..14290
                     /gene="rimM"
                     /locus_tag="KPH29_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HBG1468162.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVSREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            14299..14994
                     /gene="trmD"
                     /locus_tag="KPH29_002577"
     CDS             14299..14994
                     /gene="trmD"
                     /locus_tag="KPH29_002577"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBG1468163.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVYIYNIRDFSNN
                     KHKKVDDYPFGGGVGMVMTPQPIYDTYKHIITTHNISNPSVIYLTPKGKVYNQSIAKQ
                     MSLKEDIILLCGHYEGVDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            15179..15529
                     /gene="rplS"
                     /locus_tag="KPH29_002578"
     CDS             15179..15529
                     /gene="rplS"
                     /locus_tag="KPH29_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBG1468164.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            15850..16746
                     /gene="ylqF"
                     /locus_tag="KPH29_002579"
     CDS             15850..16746
                     /gene="ylqF"
                     /locus_tag="KPH29_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBG1468165.1"
                     /translation="MSNEYEEYLMDDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINNIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALRLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIAATV
                     LNEFRDGKIGNITLEVPENVKR"
     gene            17135..18427
                     /gene="brnQ"
                     /locus_tag="KPH29_002580"
     CDS             17135..18427
                     /gene="brnQ"
                     /locus_tag="KPH29_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBG1468166.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFALSYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLLVSILTVISSYGVDVPVV
                     KSLPLHSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT         7878 a         1731 c         3442 g         5569 t
ORIGIN      
        1 ttctttatct gttccgatgg ggtcttgtag agatacttga gttttatttt ttttgttaga
       61 tctaatattc attaaaatct cattttcaat acatctagaa gcatatgttg ctaatcgagt
      121 tcccttatct atgttataag tttctatagc tttaattaat cctattgtac ctatggatat
      181 taaatcatcc tgatcttccg ttgaattatt atatttcttt gctatataag ctactaatct
      241 catgtttctt tcaattaatg tatcttttgc acttttatcg ttttctattt taaatttagt
      301 taaatactca atctcttctt caggggttaa gggtttttca aaagatttaa gagctgccaa
      361 taggtcaccc ccttttcaac ggtcacttta aattatatga aggagactta tccaagttga
      421 ctgttatcta ataaaataat atgcatattt ttagaattta aaacatatat ttgcaaactt
      481 ttgaatggaa tatgacataa agagtgggaa tttaaattta agtttttgtt gtatatatat
      541 gaaacaaggc gaagaaatta ttgatttata aagtataaat tggaattgag gtgtcaataa
      601 atataaaagg aaatagtatt tatatatgtt ttggtgttga ggtaaaagat taatgcaaag
      661 cattagagtc tataaaaata tgaatatagg aggtgtaatt ttacaatact taaaaaagta
      721 aatgtaatta attgtttgta taaagttgat tacaagcaaa aagtatcagt catagttaat
      781 tctgttaagg atgaaggtgg tgtgttaatt tatatatgta tgggaagaaa ttttgattac
      841 aatgaaaaaa cttacaaaag atatttatga aaaaacttat agttaaatgt ttatatataa
      901 aatattattt aggggttgtt tttattattg aaaataagaa agagtggttt tatatcaatc
      961 gaatgtataa taagtattgc tatattatat gtggctgttt atttagtttc tacatcattg
     1021 tataattgtt atagttttat cagtagaaat atatctgaca gagaaatgtt aagtacagca
     1081 aaaaaatata tagaagatga gaagtataga atacaaaata gtaagtatga gttaattgaa
     1141 gataagatag aaaaaaatta cataaatgga tatgaaatta acagtagaat agagcaaatt
     1201 ttagattatt atcaatgcta tgaaataaat atagagataa aaaatgaatt taaaaaactg
     1261 aggtttaata gctatgttac tagaaaataa aggctctata ttaataataa cattgataat
     1321 attttctatt ataagtactg tgtgcattat gtgtataggt ttaatttata cacataataa
     1381 tatgtttgag ttaggataca aggacattca actaaaagaa atttctttaa gtggaataga
     1441 aatatcagta agtaatgtac tttcttgtgt tgaggaagct attgatgtat ctaataatga
     1501 agacgaattt agagaatatt tcttaggaaa taatatgatt gaatgtataa aaaaaataaa
     1561 agatacatct tatagtagtt tagaagaact ttctttaaaa gtagaaaata atactattta
     1621 tgacagagga gatttttttg aatttgatat agaaagtaat gttagagaaa aagaattttt
     1681 aaaagcaata aaagtaagag tgaaaatcaa aaatccatgg ataggaattg acattgaaga
     1741 ttatatggat aaatctaata acaataaaat tgatgacaaa acattcaata attataaagc
     1801 atctgaaaat aatgaaattc taaatcaatt taaaaatgaa agtgaaaata taaggaatca
     1861 agaggttgtt gaatttacag aagaagatga agaatatgaa gaagaactta aacaaaacat
     1921 aattgaaaat ataaagaaga attttgatga aaaaaaacta gtacaggtat ataagtatag
     1981 gggtgtataa ctttgaaaaa agaaggaatg tgactgtaat agaattaata gtatctatca
     2041 gtataatact cttgatagtt acattatctt ttccaaaaga taatcttgaa aatcataaaa
     2101 taaatttatt tgcacgtcaa ctttgttcag atataaggta tgttaggcga attaatatgt
     2161 ttagcaacta caatacatat atatattata taaatcaagg taaacacgaa ggttatgtat
     2221 tgagaaaaga tggtaaaaac attaaatcaa taatgctacc taaaaatgca aaaatagcta
     2281 gaagtattga aataataaag tttaaatctg atgggtcgcc tcaaaaggcg tctacaatag
     2341 agatatataa taatagatta agaaaaacta taacaataac acctgtaagt ggaagagtac
     2401 ttctgaaaga gggaaaatat gaaacataaa tatggttact tgcttttgga aagtgtagta
     2461 tcattgtctt caatggtgat aattatacta gttttatatt ctatattttt gtctacaata
     2521 aacttaaagt taaaagtaga agataagata gaactacaac aacaaagtct tgaaatcata
     2581 aaatctatgg aaggaattat tagcaattct atgggaataa tgaatgtatc taattatgaa
     2641 gaaacattta aaaagacaac gtctataaaa tgtagatatg tagatgaaaa taataatgaa
     2701 gaaagcataa gtaataaaga aattatattg aatgaaagac gaaataagtt atttgtaaat
     2761 tctttaaatg gcgaaagtag tcaagcaggt ggttatgaaa ttggagatta tgtagatgag
     2821 atgtatgtat taattactaa taatggtcaa tatgttaata ttaaattgaa attatcaaaa
     2881 cgtagtcaaa aatatgagac tgatttcaaa ataaaagtat ggaattttag tgaaagtata
     2941 taaaaataca taaatttaag ataaagaaat gacatcaata agtcatataa aatataatta
     3001 ttgagaacta tataactaaa taatatcaat atgtaaagtg tatagattaa gaatatagag
     3061 gataataata aaaatataga ataagtaatt tgccaaatac ttgtttatat tgtataactt
     3121 atgatattat gatattagta aaaatttgta atcctgactt gacagaattc aagtaagaga
     3181 tataatttta aaggcatagt aatgtaaaat ttactaaaaa ataagaaatt tattttaata
     3241 tggaggaaca aaaaatggta tcagcaggtg attttagaaa aggtgttaca tttgaaaaag
     3301 atggacaacc atgtttagta gttgattttc aacacgttaa gccaggtaaa ggagctgctt
     3361 tcgttagaac taaatacaaa aacttaaaaa caggagctat aagagaagaa agtttcaatc
     3421 caagtgaaaa attccctaag gctgttatag atacaagaca aatgcaatat ctatacaatg
     3481 atggtgagtt atattatttt atggatcaag aaaattttga acaaatacca ttaaactatg
     3541 agcaagttga agatgcaatt aagttcttaa aagaaaatga agttgctaca ataagatttt
     3601 accaaggaca accattccaa gtggaagcgc caaactttgc agagctagaa gttacagata
     3661 cagagccagg tataaaaggt gatacagcaa gtaatgtaac taaagcagct acagttgaaa
     3721 ctggagcagt tgttcaagtt ccattattta taaatactgg agataaagta aagatagata
     3781 ctagaacagg tgaatattta tcaagagtat aaaaatttat gtatataaaa gcttatatgt
     3841 cttaataata taagatatat gagcttttag tttatatata aaaaagaaag cgaggggtgt
     3901 tacatgaaac atttatatga agaagttgca tacttaaaag gcctagcaga aggactagaa
     3961 ataagtgctg aaagtaaaga agggaaaatg atacataaaa tagttgatgc attagaagta
     4021 tttgcagatg ctattgtaac attggatgaa gaacaagaag aacttcaaga ttttgttgaa
     4081 tctatagatg aagatttagc agatttagaa gaagatattg ctgacatgga agaagatctt
     4141 tatgaagaag atgacgatga ggatgatgag gacttcagct atatagaaat ggaatgccca
     4201 aattgtggag agttagtaga aatagacgaa gatttgttat atgatgatga agtagatgtt
     4261 gtttgtcctg attgtaaggc tgtgatatta tcatcagaag atgattgtga tgatgattgt
     4321 acatgtggtg gttgtccaag ttgtgatgat agagagtaaa aattcataag aattaaaact
     4381 gcctatgatg cctatgagat attttgcttc ataggtagtt ttatttttat gaggaagggt
     4441 attatgtatt ttggttttgc aatacttgga caaaaatagt acactagaat ttatagaata
     4501 ggattttgga gggatgtaaa tatgaaaata agtaaaaaat tactagataa tatacaaaga
     4561 tttgaaaatg ttataaatta taaatttaaa aataaagaat atatactaga agctcttact
     4621 catagttcat attctaatga aaataaaaaa tataatttta acgaaaggtt ggaattttta
     4681 ggggattcgg ttttaggcat agtgataagt gattatttat ttaatgaaga agctaatctg
     4741 cccgaagggg aactaactaa acttagagca aatatagttt gtgaagattc gttaagtgaa
     4801 gtagctaatg atataaattt aggtattcat atgttattag gtagaggaga agaagcaaca
     4861 ggaggaagac ataggacttc aatattagca gacgcatttg aagctgtaat agctgctata
     4921 tatcttgatg gaggttttga aagtgcaaga cagtttatac ttcatcatat ggaaaatata
     4981 atttatgatt caagaaaagg aaatatattt agagactaca aaacgcatct tcaagaagtg
     5041 ttacaaggga atggagaaaa taatatctgg tacaggctta tagaagaaaa aggaccagac
     5101 cataataaaa gatttgtaat ggaagttgga attaatgacg atgtattggg tataggagaa
     5161 ggaaagagta aaaaagaagc tgaacagtta gctgctaaaa tagctttaaa gaaaaaatta
     5221 tgggaaaaat aaatataata tgtaaaaata taaaaagaaa tatttatatt agaaggatgt
     5281 tgtagatgaa aaagagaatt atacctatat ttgttcctca caaaggatgt cctcatgatt
     5341 gcatattttg taatcaaaaa aaaataacag gtgtgagtac tgatgttact agtgaagatg
     5401 caagaaatat aattgaggaa tgtttagaga ctatagataa agatgcagat gtagaaatag
     5461 cattttttgg tggtagcttt actgctatag atattgatat acaaaaaagt ttattatcag
     5521 ttgctaaaga atatgtagaa aaaggtcttg taaaagatat tagaatgtct acaagaccag
     5581 attgtattga tgaagatata ttaagtatgc taaaagaata taagacaagt ataattgaac
     5641 taggagtaca atccttagat gaaaaagtat tattagatag tataagaggt catcaatctg
     5701 aaattgtata caagagttca aagatgataa aaaatagtgg aatcaaactt ggattacaga
     5761 tgatgattgg tcttccagct gacacagaag aaaagtgtat atttacagca aaaaaatttg
     5821 tagagctaaa accagattgt gttagagtct atccaacttt ggtagtaaaa gatacaggtc
     5881 ttgaaaagtt aatggaacaa aataaatata atccatttac tcttgaagag agtgtaaata
     5941 tagtaaaaaa agttcttgta ctgttttatg ttaataatat caatgtaata agagttgggc
     6001 ttcaagctac tgatgatata caaattggaa aagctgtttt agcaggacca tatcatcctg
     6061 catttagaga gttggtagaa gcagatatga taaaagatta tttagaattt gttattttgc
     6121 aaaataaaaa tataaagcaa atgttggtca aatctaataa aaagaatatt tctaaaatca
     6181 taggaaataa aaaaacaaat gttaaatata tggaagaaaa atttggtgtg cttttaaaaa
     6241 cacaagaatc cgatttagat ataaatcaat tagaaattgt gttagatggg aaaagtttaa
     6301 ttagtgctaa tatgagagac attcatagaa aattgtatga catttatgac ctttagtttt
     6361 aataatgaag ggagagaaaa gatttgtatt taaaaagact agagttaaag ggatttaaat
     6421 cctttccagt aaaaacagat attattttta aagaaggaat aacagccata gtaggaccaa
     6481 atgggagtgg aaaaagtaat atatctgatg ctgtaagatg ggtattagga gaacagagta
     6541 ttaagagtct tagaggtgat aaactcgaag atgtaatatt tgcaggtaca gatacaaaaa
     6601 aacctatgaa ttattgtgaa gtggctctta ctattgataa ttctgaaaat caattggaat
     6661 tagattttac agaagtaact ataagaagaa gggcttatag aaatggtgaa agtgaatttt
     6721 ttctaaataa taaaagttgt agattaaaag atataaaaga agtcttttta gatactggta
     6781 ttggaaaaga tggttattca atcatagaac aaggaaaagt agatgaaata ttaagtaata
     6841 atcctttaag cagaagaaaa gtttttgatg aagcttgtgg catatcaaaa tatagatata
     6901 aaaaacagga agcagaaagg aatttaagta atacaaagga aaatttagaa agaatagatg
     6961 atgtatatat agaaattgaa aatcaattaa agcctctttt taatcaacag acaaaggcaa
     7021 aaaagtactt ggaaataagt gaaaaattaa agatacttga agtaaatagc tttataagag
     7081 aaattgaagg aatagaaaaa gaattaagtg aggttaatga acatcgtaag gttattgaaa
     7141 aggagctcaa tgaaaaagaa gaacaaaaaa atgttgtaga gaaaaagcaa gaagatatta
     7201 ataaagaggt agaggtatta caagatgtaa ttgaaaaatc tgtagattat ataaattcaa
     7261 ttaaaggagt tatatcaaaa aaagagtccc aaataaattt aattaaggaa agaataagaa
     7321 attttacgaa tgaaattagt agaaaaaatt tagaaataaa tgatataaaa gagaagctaa
     7381 atgaaaataa gcaatacata aaagaattag aaagtaataa attatcaggt agtgaagaac
     7441 tatctacctt gcaggaaaat ataaaagtat tggaggcaag taaagataaa caaaaaatta
     7501 agttagaatc cctaaataat gagatagaat tattgaaaga aagtataatt gatatattaa
     7561 ataagaaaca agagtttagt aataagctgt ccactttaaa cgcaaataaa gaaaatatga
     7621 atatcagaga tgaaaatata aattcagaga taacagagtt aaacaaaaat attgaaataa
     7681 aatcatctga attagatact ataaataaag aatttaatat gcaaaatgaa aatttaaaga
     7741 atgtaaataa tagacataaa gaattatcaa ttaatttgca ggattcaatt agtgaacaca
     7801 ataaactaga agatgaaata cagaaaagta aatataattt aaatggatat aattcaaagt
     7861 taaatgtcta tatagatatg gaaaatcatt atgaaggatt taatagagga gtaaaagaag
     7921 tactaaaaaa taaaaattta aaaggagttc atggagcatt aggacaaatt ataaatgtac
     7981 ctgaaaaata tgagaaatct atagaagcag ctcttggtgc ttatatgcaa aatataataa
     8041 ctgataatga gtttagtgct aagtctgcaa taaactatct aaaacagaat aatttaggta
     8101 gagtaacatt tcttccatta aatataatta aatcaaataa agtaagtcta ggaaatttaa
     8161 aagcaaatac taagttcata ggaatagcaa gtgatttaat tacatttgat gaaaagtata
     8221 gaaatataat tgaaaatata cttggaagaa caatacttat aaataatata gatgagggca
     8281 taaaatttgc aaaagaaact ggtcataggt ttaaaatagt aactttagat ggtgaaatct
     8341 taaatccagg aggttctttg actgggggaa gtctaaaaac taatgggaat atactatcga
     8401 gaaaaagata cattaatgaa tatacagaaa agataagtaa tatcaagaat gaaatttcac
     8461 atctagaatt aaaaagagaa tctttagaca aagatgtgaa aaatatcaaa aatgaaattg
     8521 attcacatga aagtaaaata aaagatttag aaaaaagtat aataataaaa agtacaagta
     8581 taaagaatgt tgaatctgaa attgaatcgt taaaaggtag tattactaaa ttagaaaatg
     8641 agaagaatga tttaaattct aatctaaatt atacacttga aaaaagcgat gatgttagaa
     8701 aagatatgga agagttagat gatttataca ataaaaataa agaaaaaatt gatgcattga
     8761 atgaggaaat aaaaagatac aatgatttat atgataaaga aaaatctgaa tttgatgaat
     8821 tgaacttaag tttagtgaag aaaacagaag cttataatag tatagttaga gatataaaaa
     8881 gaatatctgg tgaaaattgt gagctagaag aaaagaataa acaattagaa gagtcattaa
     8941 attatgaaga acatgaaata attaaattac aagattctat agccactgaa gaaaaagaaa
     9001 aagaaaattt aacaaaacaa ctaggggata gtaatagaaa tcttgaaact agaaaaatag
     9061 caaaagatga tttaaaaaat agttttgatg aaattaataa agaattaaaa actatagata
     9121 gacagcatat agagcttaaa gaaagtttat ttaaagttgg tggaagatta gaaagattaa
     9181 aaactagtca agacacatat ataaataagt tgtttgagca atatgatatg actttagtac
     9241 aagcattgga aattaaagat gaagatttgg atatagatag aaaattctta gaaagtctaa
     9301 agagagaaat aagaagtctt ggaaatataa atataggctc tataaaggaa tatgaggaaa
     9361 taaaagaaag atacgatttt tatagcgaac aaaagcaaga tttagaagag tctatggaag
     9421 agatagaaaa acttatacac actttagaag aaaatatgaa atctgaattt gaaattaagt
     9481 ttgaagaaat aagtaaaaat tttaaatatg tatataagag attatttggt ggaggttgtg
     9541 gagaattaac tattttagat aaagaaaatt tattagaaag tgatatacta ataacagctc
     9601 aacctccagg gaaaaaaatg aaaaatttaa atctattatc tggtggagag aaagctttaa
     9661 cagcaataag tatattattt gctatattga ttacaaagcc aacaccattt tgtatattag
     9721 atgagattga agcgccactt gatgatgcaa atatatttag atttggagaa tttttgaagg
     9781 atttatctaa agagactcaa tttatatcag taactcatag gagaggaact atggaagctg
     9841 ctgattatat ctatggagtt acaatgcaag aaaaagccat ttcaaaagtt attagcttaa
     9901 aattaaaaga agctcaggaa ataacagata ttatataact tagattgaca taggaggaat
     9961 aacagtgtta aaaaaattat ttggttttgg aaaagacaag gaaaaagaaa tagaaaaaaa
    10021 agatgctgaa gaagaaatag aagttgaaga cagtgttgat agtttagaaa acttggaaga
    10081 aaccattttt tcaggacttg aagaagaagt gatagataaa gtggaagata ttgaagaaaa
    10141 aactgaggaa agtgtcaata atgaaggcac tgaagaaatc gaaaattttg aaaaaataga
    10201 agttgagatg gatagtaatg caaaagaagt agaaaatatt tcaaataatg attctcaaga
    10261 agagataatc gaagaattag aaaattatga tgaaattgag tctaaagaga tagaagataa
    10321 agaagataag aaagttaact tgtttgaaag actaaaacaa ggattgacaa aagcaaagca
    10381 aggaataact gatagaatag atgaagtttt aaaatcatat acaaaaatag atgaagaatt
    10441 acttgaagat ttagaggaaa ttttaataac agcagatgtt ggtgtaaata caactatgga
    10501 tataattgaa agactaagag ataaaataaa gcaaaaaggt attactgaac caataaaggt
    10561 aagagaagaa cttaaatcta tagttgaaga tattcttaca aatgaaaact ctacactaga
    10621 tatagaacca gcaccatgta taatattaat ggtgggtgta aatggggtcg gtaagactac
    10681 tacaataggt aaactagcaa atagatacaa aaaagatggt aaaaaagttt tattagcagc
    10741 agcagataca tttagagcag cagcaacaga gcaattagaa atttgggcaa atagaaccaa
    10801 tgtagacata ataaaacatc aagagggagc agaccctgga gctgtagtat ttgatgctat
    10861 aaaagctgca aaagctagaa aaacagatgt attaatatgt gatacagcag gaagattaca
    10921 caataaggct aatcttatga atgaacttgg aaaagtattt aaaatagtag atagagaatt
    10981 tccagaagca aaacgtgaag tacttcttgt ggtggatgct acaacaggtc aaaatgcagt
    11041 tgttcaagca aagacattta aagaagttgc agatataaca ggtatagtac ttacaaaact
    11101 tgatggaact gcaaaaggtg gagtagtcct tgcagtaaaa tcagaagtag atgtacctgt
    11161 aaaacttatt ggagttggag aaagtgtaga agacctacaa gatttcaatg caaaatcatt
    11221 ttcagatgca ttatttggta actaatgttc ttttaaaaat aatggttgac aagagtgttg
    11281 ttatgatata aaatacaata gtgtaaagta aataaccttt acagtatact gctaggaagt
    11341 gtaagatatg aatatagaaa aaatggtcga aattggattg ttatttgaac agtataaaga
    11401 attattaact gacaagcaga aagaaatagt tgctctatac tatgaagaag attattcttt
    11461 aggtgaaatt agtgaaaatc tgaatgtatc aagacaaggt gtttatgata ccttaaagcg
    11521 ttcagaaaaa atactaagag attatgaaga aaagttacac ttagtatcaa agattcaaga
    11581 gcaggaaaaa aatattaaga ttataaaaga taaaattatt gacattaaag aagatttatt
    11641 gcacaataga gattgtgcta atttaatccc taagctagaa aatatagaag atgtatgtag
    11701 ggagatgata aaatgatatt tgaaggattg tctgataaac tacaaggggc atttagtaag
    11761 ttaaaatcta aaggaaaact tacagaagta gatgtaaaaa atgctatgag agaagttaaa
    11821 cttgctcttc ttgaggcaga tgttaacttt aaagtagtta aagatttcat aaaaaaagtt
    11881 caagagagat gtgttggaca agaagttatg caaagcttaa ctccagctca gcatgtaatc
    11941 aaaattgtaa atgaagagct tacaagttta atgggagatg tacaaagtaa agtaatgatt
    12001 tcctcaaagc ctcctacaat tttaatgatg gttggattgc aaggtgcagg taagactact
    12061 acagcaggaa aacttggagg atattttaaa aagcaaggta aaaaacctct attagtagct
    12121 tgtgatattt atagaccggc agctataaag caacttcaag ttgttggaga aaaattggat
    12181 atacctgtat tcaatatggg agataaagaa aatccagtaa atattgcgaa agcaggttta
    12241 agccatgcga ttaaaaatgc taatgattta gttattatag atacagctgg tagacttcat
    12301 atagatgaag tacttatggc tgagttaaag tctattaaat cagaagttaa gccacatgag
    12361 atacttttgg ttgtagattc tatgacaggt caagatgctg taaatgttgc agaaagcttc
    12421 aatgaggcac ttggtgttga tggagtagta ttaactaaat tggatggaga tactagaggt
    12481 ggtgctgcac tttcaataag agcagttaca caaaagccta taaaatttat ggggatggga
    12541 gaaaagctag atgatataga acctttccac ccagatagaa tggcatcaag aatattaggt
    12601 atgggagatg tcttaagttt aatagaaaaa gcacaagaaa atatagactt agagaaagct
    12661 aaagaattag agcaaaaaat taaaaaacaa gagcttgatt ttgaagattt cttacaacaa
    12721 atggaacaaa tacaaaatat gggacctctt gacaagatac ttggtatgat acctggtatg
    12781 ggaaatgtaa aagaccaact tggagatgta gatttgaatg gcaaagaaat gaagagaaca
    12841 aaagccataa ttcagtcaat gactacatat gagagaagaa acccaggagt attaaatgct
    12901 tcaagaaaga aaagaatagc taagggcagt ggaactagtg tacaagatgt aaatagactt
    12961 ataaaacaac ttaatgaaat gaaaaaaatg atgaagatgt ttgctggttc acaaaagagt
    13021 atgaagaaaa ggggcggctt aggcggttta ccttttttta aataaaatat aaaaaagtaa
    13081 aaattattcg gaggtgactg taatggcagt taaaataaga ttaaaaagaa tgggagctaa
    13141 caaaaaacct ttctacagaa tagtagtagc tgattcaaga gctccaagag atggaaaatt
    13201 tatagaagaa ataggatact ataatccaat ttctgaacct aaacaagtaa gaataaatga
    13261 tgaaaaagct ataaaatggt tagctactgg tgctcagcca acagaagtag ttaaaaaatt
    13321 acttgtaaaa aacggagtaa tagaaaaatt cgaagcttct aaacaagcaa aataattttt
    13381 tcttggcaat tagtttaagt aagggctcat tgctaaggtt actttaggag atgaaatata
    13441 tgaaagagct agttgtagat atagctaagg ctctagttga taatcctgac tcagttgttg
    13501 ttgaggaatt tgaagataat gatggtatcg tcttaaagct tacagttgct caagaggata
    13561 tgggtaaggt tattgggaag cagggaagaa tagctaaagc tataagaact gtcgtaagat
    13621 ctgttgcaaa tagagaaaat ataaaagttt ctcttgagat agtataaaat aggaggatta
    13681 ggtataatgc ctagtccttt ttatataaat gcgtagttta agattttatg atgatatgac
    13741 cacataagat taaatttttt aagaaggtga taacatgaaa gaaaaattaa ctcattttaa
    13801 ggtaggtcaa atagtaaata cacaaggttt aaaaggtgaa gtaagagttt atccacttac
    13861 tgatgatata gagaggtttg atgaattaaa agatttctat ctgggaaaag atttaaataa
    13921 taaattggct gttgaaaaag taagatacaa aggttctatg gttataatga agataaaagg
    13981 catagacagc atagagaaag cagaaaaact aaaaaataaa tttatgtatg tatctagaga
    14041 agatagcaga gagcttgaag aaggcgaatt ttttatagca gatatgattg gaatggaagt
    14101 tttaactgta gatggaaaat atgtaggaat actagaagat gttcttcaat actcagcaaa
    14161 tgatgtttat gtagtaaaag gtgaagaaga taaagaattt atgataccag ctataaaaaa
    14221 atttgtacct acaatagata ttgatgaaag aaaaatgatt atagacccta ttaagggaat
    14281 gatagattag gtggtaatat gagatttcat ataatgacac tttttccaga aatatttaat
    14341 tcgtacatgg atgaaagtat aatgaagaga gcagttgaaa aaggtataat tgaggtttat
    14401 atatataata taagggattt ttctaataat aaacataaaa aagtagatga ttatccattt
    14461 ggaggaggag tggggatggt aatgactcct caacctatat atgacacata taagcatata
    14521 ataacaactc acaatataag taatccaagt gtaatttatc ttactccaaa aggtaaggta
    14581 tacaatcaat ctatagcaaa gcagatgtct ttaaaagaag atataatact attatgtggc
    14641 cattatgaag gagtagatga gagaatcatt gatttaatag ttactgatga aatttcaatt
    14701 ggagattatg tattaactgg gggagagctc ccagcactta taatgataga ttctatatca
    14761 agattaatac caggtgtatt aaaccaagaa gaatcatttg aagaagaatc atttaaagat
    14821 aacttattgg aatatcctca ttacactaga cctagagatt ttgaaggatt aaaagtacca
    14881 gaagtgcttt tatcagggaa tcataaaaag atagatgagt ggagaagaga agaatctata
    14941 aggataacca aagaaagacg atttgattta tataaaaagt ctaatgaaaa ataaaaatat
    15001 aatgaaaaat taatatatat gtatacaata aagtacctat aaatggaatt aaaaacataa
    15061 aagtgcttgt aataactatt gaagtatagt ataatataaa atgttgaaaa tacgggcgtt
    15121 cctctgttta cactggtcgg gtagttaaga acgtctatga tatctaggag gaagaaagat
    15181 gaatgaaatg ctaagagcaa ttgagcaaga acaattaaaa aatgaagttc ctaattttgg
    15241 acctggggac acagttaaag tacacgttag aattatagaa ggaaaaagag aaagaataca
    15301 agtattcgaa ggtgtagtat taaagagaca aggtggagga gcaagagaaa ctttcacagt
    15361 tagaaaaatg tctttcaatg ttggtgtaga aagaactttc ccagttcact ctccaaaaat
    15421 agaaaaaata gaagttacta gaaaaggtaa agtaagaaga gctaaactaa actacttaag
    15481 aggtagagta ggtaaagctg ctaagataaa agaagctaga aataaataat taaaaacttt
    15541 acttataaat ggggggctga tttatcagtc ctctttctat tttaatgaaa attacagcaa
    15601 ataattatac tagaaataaa tagaaatatt tgttgaaaat atacattatt tatctatgaa
    15661 tttatagctt ttaaaatgaa aaataaaatt ggtaaatatt ggattatatt ttgtaacgat
    15721 attaaaaata agagaacagt attatgtaaa gtatgacgtt aaaaagatga caatattata
    15781 gtttgtgaag atagattttt taagtggaat tagaatataa taatataata ttaataagga
    15841 gtgatacatg tgtcaaatga atacgaagag tatcttatgg atgacaatct acatataaac
    15901 tggtatccag gtcatatgaa aaaaactaag gagttagtaa aaaataactt aaaattgatt
    15961 gatgtagtaa ttgaactttt ggatgctaga atcccgttta gtagtaaaaa tccagacata
    16021 gacaggcttg taggagataa gccaagagtt gtcgtactta ataagagtga tatggcagat
    16081 agggataaat tgaatcaatg gattgaatac tataagaaaa ttaatatcaa agctatacca
    16141 gtagatacaa taaaaggtgt tggtataaat aatataatcg aagaatgtaa aaatgtaact
    16201 agagaaaaaa tgagttcttt aaaagacaaa gggagaaaag aaagagctat acgaataatg
    16261 atagttggag ttccaaacgt tggtaaatcg tctcttataa acaaattaac aggtagaaaa
    16321 agcactcaaa ctggagataa gccaggagta acgaaaggga aacaatgggt tagattaaag
    16381 ggaaatcttg aacttttaga tacacctgga atactttggc ctaagtttga agaccaagaa
    16441 gttgcattaa atttagcatt tagtagagct ataaaggatg agattttaga tactgagact
    16501 ttggctttaa gacttataga aaaacttatg aaaatagaac ctgagaagtt gaaagccaga
    16561 tataagttag attgtctagg agaaactcca attgaaacta tggatatgat tggccataaa
    16621 agagggttta taacaggtaa aaaagaacta gattatacac gcatagccgc aactgtatta
    16681 aatgaattta gagatggaaa aataggaaat ataacattgg aagttccaga aaacgttaaa
    16741 agatagattt taaaacaaaa tcaaagattt aatgttaaaa aaataaaaaa gccttttagc
    16801 aaaggctttt ttattttttt gaaatataga caaatatctc catataaaag tataaattag
    16861 gttagaaaac ctttaaaaat attaatttaa gacaaataca cctaatattt tgaaaattaa
    16921 aatgagtgtg attattttaa attttagcat attgacttgc ttgtttcgta taatatataa
    16981 taagcacaaa taaatacaat atattaaaaa tttaatttat ttaattttat taatttaata
    17041 ataagttctt attgtttaat ttaatttatt tatgcaagcg taaacaatcg agacatttta
    17101 ttaatttaat aaggttaaaa agggggattt tataatgggt aagaccaagg atgttatagt
    17161 atttggattt gcgttatttg caatgttctt tggagcaggt aacttaatat ttccacctta
    17221 cttgggaata attacaggtc cagaatggtt aattgccttc ttaggattta cgtttgctga
    17281 tgctggattg gcacttctag cagtaatggc tacagctaaa ttcgatggaa acgtagttga
    17341 aatgtttaaa agatgtggta tgaagttagg gattcttata ggttgtgcag acattttatg
    17401 tataggtcca ttcttagcta taccaagaac aggtgcaaca acttatgaaa tgggtataat
    17461 gcctttattt ggaacttcaa ttccagtgtt attattctgt atactttttt ttgcgctttc
    17521 atatgtacta acaataagac cttctaaggt cgtagatata gttggacagt ttttaactcc
    17581 agctctttta atagctctag catttataat tataaaaggt attatatcac ctcttggaga
    17641 tatagtagat aaacctatga tacctaatgt atttgcagaa ggtataggtc aaggatatca
    17701 gacaatggat gcttttgcag caatagcact tgcatcagtg ttaatagtat ctttaaatga
    17761 taaaggatat tctactatta gtgacaagtt aaaaatgatt ggtaaggcgg gggttcttgc
    17821 ttgtggagga ttagctctag tttatggagg attatgtttc cttggagcaa ctgtatcaac
    17881 aatgtatgga acagatgcag tacaatcaca agtaatagta aatataactg aaggattact
    17941 tggaaatgtt gggaaagcga ttttagcagt agtagtatca ttagcatgtt taactacatc
    18001 tataggtctt acatctgcca caggtcaata tttctctaga cttacaaaag gtaaattgag
    18061 ttatgaaaaa atagtattag ctgtatctgt attcagtgca gtagtagcat cttttggtgt
    18121 tggaactata ataaagatag catctcctat actaagtata gtttatccac catcaattgt
    18181 tttaataatt ttagctttct tcaatgaaaa aattaagaat gataatgtgt acaaaggagc
    18241 agtatatatg tcattgcttg taagtatact aacagttata tctagttatg gagtagatgt
    18301 tccagtagta aaatctttac ctcttcattc attaggattt aactgggtgg taccagttat
    18361 aatagctgga ataattggta attttatacc atcaaagagt caatctaata cacttggaac
    18421 aaactaagtt ttgtttaatt aaaatataat caattgtaaa taaatgagtt aactaaaagt
    18481 aaaaggagtt atttgtacaa ataactcctt ttattttggg attctataat taattatttg
    18541 tatttaatat ggaaaaagta taagaaaata tcaaaaaaat atatagttta ttttttaaca
    18601 ataatagaga ttaaaaaaga
//
LOCUS       DAELXX010000079        18595 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.079, whole genome shotgun sequence.
ACCESSION   DAELXX010000079 DAELXX010000000
VERSION     DAELXX010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18595
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.079"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(157..2823)
                     /locus_tag="KPH29_002581"
     CDS             complement(157..2823)
                     /locus_tag="KPH29_002581"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBG1468167.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKAHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKVKKEKFEEMIKSVEERLTNLESKIK"
     misc_binding    complement(2899..3161)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3462..4289)
                     /locus_tag="KPH29_002582"
     CDS             complement(3462..4289)
                     /locus_tag="KPH29_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG1468168.1"
                     /translation="MKIKDNKAVFHVFIRIFLIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     gene            complement(4337..5773)
                     /locus_tag="KPH29_002583"
     CDS             complement(4337..5773)
                     /locus_tag="KPH29_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HBG1468169.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     DLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            complement(5860..6729)
                     /locus_tag="KPH29_002584"
     CDS             complement(5860..6729)
                     /locus_tag="KPH29_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBG1468170.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGNYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            complement(6887..7540)
                     /gene="phoU"
                     /locus_tag="KPH29_002585"
     CDS             complement(6887..7540)
                     /gene="phoU"
                     /locus_tag="KPH29_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBG1468171.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            complement(7568..8332)
                     /gene="pstB"
                     /locus_tag="KPH29_002586"
     CDS             complement(7568..8332)
                     /gene="pstB"
                     /locus_tag="KPH29_002586"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBG1468172.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            complement(8351..9175)
                     /gene="pstA"
                     /locus_tag="KPH29_002587"
     CDS             complement(8351..9175)
                     /gene="pstA"
                     /locus_tag="KPH29_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBG1468173.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVIIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            complement(9177..10094)
                     /gene="pstC"
                     /locus_tag="KPH29_002588"
     CDS             complement(9177..10094)
                     /gene="pstC"
                     /locus_tag="KPH29_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBG1468174.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            complement(10476..11711)
                     /locus_tag="KPH29_002589"
     CDS             complement(10476..11711)
                     /locus_tag="KPH29_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1468175.1"
                     /translation="MRIFYLEIKRVLHSRRTLILLLLALVLSALMAFLPITYEGINYL
                     DNNGKVVELSGLDAIKYKKTIYANNSEISSGVVTPKKLKHALNIWKKTEEKYGKVGSE
                     DFPLDIYSKSILPIRPLLNRLPEAFANSETGFATELSEINTNDLDSFYEQCKTHLNDI
                     MKIERQTSSAQMKAQELYRKVDTPFEIYPGYSKDAFDYIIFYIFILLIICVAITAPTF
                     SGEYQTQSDSILRCTKHGHTRLAFTKILALFSIFIVMFVVCISIHLAISDLAFGTECL
                     KTSFQMLFSVISLVNINLLQAQILIAIGGLISILAMVSLTLFLSAKCKDSLTSMLIAF
                     SICLIPMGIGVSWIGYLLPPGGIGLKISLLYQMTSFNFLHLGGMSFWTPHIILFFSVI
                     EIPIFIFLAIRTYCKHQVS"
     gene            complement(11712..12599)
                     /locus_tag="KPH29_002590"
     CDS             complement(11712..12599)
                     /locus_tag="KPH29_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1468176.1"
                     /translation="MELKIEHVTKKFKDKIAVEDVCLTLTPGVWGFLGANGAGKTTLM
                     RIITDIMKPTNGKILYNGKNIYIMGAEYRNLLGFLPQEFGFPQDFSVKDYLEYIAALK
                     DVPPREVKGKINNLLEILTLSDVKNKKIIKLSGGMKRRVGIAQAMLNDPKILIMDEPT
                     AGLDPGERVRFRNFISEFSHNRVVLISTHIVSDIEYISTQNAIMKSGKILDVGTTDKL
                     VKKVEGKVWECLISNRDISKYEMNLRIINQRSEKNNQVSIRYLAEKSQIDNSIAVEPR
                     LEDLYLWLFPEAVYSKEGK"
     gene            complement(12744..13859)
                     /locus_tag="KPH29_002591"
     CDS             complement(12744..13859)
                     /locus_tag="KPH29_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1468177.1"
                     /translation="MKKINNFTYRKLRDKYKFIKREKNTEAYLKYKLYIMLAIYTGIV
                     LVLMFMADYTFSNFSNPLVKWLKLRRDVLFFLVFIIGYITIFNFYWKKPWNYLREIIN
                     ATHTIYQQNDELVELSKPLKEIEQQMNQIKMSVLLSQQAVKEAENKKNNLVMYLAHDI
                     RTPLTSVIGYLSLLKEIPNMDNSQKSKYTEVALDKAQYLEKLINELFEITRYNSNQIK
                     INFEKVDLYYMLLQLKDEFYPLLSEKGNRAILKANESLTIKADSEKLARVFNNILKNA
                     IAYSYPNTEIIISAVHKDSKVDIAFRNYGQTIPEEQLSAIFEKFNRLDEARKSDMGGA
                     GLGLSIAKEIINLHGGDITVQSKDEVTTFTISLPTSI"
     gene            complement(13895..14593)
                     /gene="vanR"
                     /locus_tag="KPH29_002592"
     CDS             complement(13895..14593)
                     /gene="vanR"
                     /locus_tag="KPH29_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HBG1468178.1"
                     /translation="MSGKILVVDDERDIADLIEVYLTNENYIVYKFYSGLDALNCINL
                     NEFDLAILDVMLPGVNGFSLCQKIREKYTYPIIMLTAKDEETDKITGLTLGADDYVTK
                     PFRPLELVARVKAQIRRYKKYSGSLQSGEELSHIFSFLGLELNTQTYECHLNGKALSL
                     TPTEFSILHVLLKNKGSVVNSENLFHAIWKDEYYNKSNNTITVHIRHLREKLGDTVDE
                     AKYIKTIWGVGYKI"
     gene            complement(14975..15655)
                     /locus_tag="KPH29_002593"
     CDS             complement(14975..15655)
                     /locus_tag="KPH29_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1468179.1"
                     /translation="MQTKILIIDGDKDNCQKLKGFLEEKEISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILTDGDGWTLCKKIRNVTTCPIVYMTYINEEQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            complement(15705..17720)
                     /locus_tag="KPH29_002594"
     CDS             complement(15705..17720)
                     /locus_tag="KPH29_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG1468180.1"
                     /translation="MKKYNILKGTLIVLIVSLLAIAGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGN
                     QSPYGNATYRMRIKNEGEDKLLSLELPEIFSASEVWINGEIVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKDTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKLYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDSSY
                     FMVVLCIICINGSISKIEEDKVYRFLILPLGITMSIISIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFIIVILFSLMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KKFLADVAHDLKAPVSAIQSFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNDFIKEIYENNLPDTQACGVNFETYILEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKVSDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLCFL"
     gene            complement(17792..18469)
                     /locus_tag="KPH29_002595"
     CDS             complement(17792..18469)
                     /locus_tag="KPH29_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1468181.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVERI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
BASE COUNT         5943 a         3332 c         2011 g         7309 t
ORIGIN      
        1 taaattttaa gacataaaat tcatatattc acaacataaa ataaaaatat aacacatctg
       61 taaagaatta taacatcata tttgtatata tataaagaaa aacatacaaa aagtatagaa
      121 tatatacttt ttgtatgttt ttactaattc ttatatttat tttattttag attctaaatt
      181 tgttaatctt tcctcaacag atttaatcat ctcttcaaat ttttcttttt ttactttttc
      241 ttcttcaatt aaactttcag gagcttttgc taagaaacct tgatttgcaa gctttccatt
      301 aactctcttt atttcgcctt ctaacttttt cttttcttta gacaatctct caatttcttt
      361 ttcaaaatca actaattcat taagaggtat aaatatttca acaccatcaa taactactcc
      421 aactgcatct tctggaacat tagatttatc ttttgctatc tctacatttg aagcagatgc
      481 tagagttata aaatagtctt taccaagctc aatagcttct attttttctt cacttgggat
      541 tattatcaat ttagctttct tagaaggtgg tacattcatt tctgctctca catttcttat
      601 acttcttata ccttccatca ataagttcat catatcttct tcttcagcca tgttatcttc
      661 ttcattatat tttggccatt cagatactat tatacatcct tcaacagtag gaagatgagt
      721 atatatttcc tctgttataa atggcatgta tggatgtaat aattttaata ttttttcaag
      781 aacataagtt aatgtatata atgctgcaga tttagcattt gcatcttccc catataatct
      841 aggtttaacc atttcaatat accagtcaca gtattctgac caagtaaaat cgtatatctt
      901 ttgaagtgct attcctaaat caaacttatc catgttatta gttgcttctt ttacaatatt
      961 atttgctctt gatattatcc atttatcagc tagagttaag tcctctttaa cactttctct
     1021 agtcatgttt ttaattatat cttcatcaat attcataaat acaaatcttg atgcattcca
     1081 caacttattt gcaaagtttc tagcaaattc tactctttcc atatagaatc tcatatcatt
     1141 tcctggggaa tttccagttg ttaatgtaaa tcttagggca tcagctccat attgctcgat
     1201 tatctccaat gggtctatac catttcctaa agatttactc atttttctac cttgagaatc
     1261 tcttactaat ccatgaacca aaacatggtc aaatggcttt tcattcatac agaacatacc
     1321 tgcaaatgcc attctaacta cccagaagaa tattatatcg tatcctgtaa caagtacact
     1381 tgttggataa taataatcta aagcttcagt cttatttggc catcctagag tagagaaagg
     1441 ccataatgca gaactgaacc aagtatctaa ggcatcttca tcttgtttaa aatgagtgct
     1501 tccacattta ggacacttat ctggcatttt tctagcaact actatttctc cacactcttg
     1561 acaataataa gctggtattt gatgacccca ccataattgt cttgatatac accaatctct
     1621 tatattttct agccattgta aataagtttt atcaaattta tctggaacaa attctaattc
     1681 tcctttttta agaatatcta tagctggttt tgcaagttct tccatcttaa caaaccattg
     1741 ctctgataat ctaggctcaa ctactgtatg gcatctataa caagttccta cattatgatt
     1801 atgcgctttt atcttaatta ggtaaccttg ttcttttaag tctgctacga tagcttttct
     1861 acactcaaat ctatccatac cttcatattt tccacaaact tcattcatag ttccatcttc
     1921 attcatagta tttaactgct caagattatg tcttaatcct acttcaaaat cattagggtc
     1981 atgtgctgga gtcatcttaa ctgcaccagt tccaaactct aaatcaacat aactatctgc
     2041 aactattgga agttctcttc ctactaatgg aagtattgct gtttttccta ttaaatgttt
     2101 atatctatca tcttctggat ttactgctat accagtatct cctatcattg tctcaggtct
     2161 agttgtagct atttctaaaa agtcttcact atcttttagt gggtacttta tatgataaaa
     2221 gtttccgtcg tgttcttcat gttcaacttc tgcatctgat aaagtagttt tgcaatctgg
     2281 gcaccagttt attattctat ttcctctgta tatgtgacct ttttcatata ggcttacaaa
     2341 aaattctata actgcttcat tacacccttc atccatagta aatctttctt tatcccaatc
     2401 acaagaatca cctagttgtt taagctgatt agatattttt cctccaaatt catctttcca
     2461 ttcccaggct ctctttaaaa attcttctct acctatttca tactttgtct tgccttcttg
     2521 tttttttatt ctttcaacaa ctttaacttc tgttgctata gaagcatggt cagttcctgg
     2581 aagccaaaaa gcttcatatc catccatacg tttccatctg attaatatat cttgaagtgt
     2641 atggtcaaga gcgtgtccca tatgcaattg ccctgttata tttggtggtg gcatcattat
     2701 tgtaaatggt tttttatctg gatttggttt agatttaaaa tacccttcat ccatccattt
     2761 tttatatagc ctagcctcaa aatcttttgg gttataagtt tttgataaat tcgtattttc
     2821 cattgtcctg cctcctgtat tttataaata aattttttaa atatctttag aaataaaaaa
     2881 gagctcttca tcctctttaa aaaggacgaa aagctcgcgt taccacctta attccaaagc
     2941 ttaaaataca ttaacatata attaaattat tagttaatgt tattttatca actttgactc
     3001 tcaaagaatt taacggttct aaccgtctaa acttacttat agttcagttt agaaactcca
     3061 aagctacatt cagtaaatct tatacttaga aaatcttaca gccagtgaat tttcctctct
     3121 tttagtagta tttacttact cctcttcttc tcagtcttta atattacata ttattatata
     3181 caaatattat tctctttgtc aattattttt attttatttt ttctgaattt tcttaattat
     3241 gtctattctc ttttatatag ttgttatatt ttaaaatatg tttttacagt tattctgtct
     3301 tataatgtca ataattgcaa gattatttta ttttaatttt aatgctttct cattaatttt
     3361 atattatatt atccttacag tatacctctt attcgtattt tttatattat atgttttatt
     3421 ttataaataa tttactttat cttttactat atttaaattt attataagtt ttcttgccag
     3481 aaatttttag gtgttatatt ttctgtaatt ggcaatattg tatatcctct ctcttttaag
     3541 attcttataa tattatttaa agcatttaaa ctttgctctt tctcatgaat taatactact
     3601 aattcatctc tttttcttcc ataatataat atattactta ctatctgact tgtagaagac
     3661 ttccaatcct gtgtatccaa attccagtcc caaattaaat aatcatttgc cataagttta
     3721 ttatatgaac cttctggcat atatggttta cttccgtatg gtaatcttat taattttgaa
     3781 ctctgaccag ttatttcttg tagagtatta ttgcatgtat taaattcccc aactgttact
     3841 tctgggcttt tatataactt atgtatatca tgacttacac tatgaaaacc tattgagttt
     3901 tcctcttcca agactctcct tacttcttcc ttgtgatttt tcatattttg attaagcata
     3961 aaaaatgtcg ctttgacatc attttctttt aaaatatcaa gtagtgcatt tgtatatttt
     4021 gatggaccat catctattgt tatgtatgct actttttcag aagtagcccc tggacctttt
     4081 gtttcttctt gagatgcttt gtatatgatt tctcctaaag tattactttg agaaagcagt
     4141 tccagtgtat tttcaccttt tgaacttata ttttcaactg gaacattatt aattttttcc
     4201 tccaataatg ttccagttaa aattaaaaca gctataagaa atatccttat aaatacatga
     4261 aaaacagcct tattatcttt aattttcatc ccccatatct cccttttata aaagcataag
     4321 tttaagactt aaaaagttat tttttatatt tattctcttt cttattactt tttatatctc
     4381 tactaaatat ttttttaacc ttttcttctt ctttttctcc taaagattct actcttttga
     4441 ttcccaaaga agttaaaaat tgtaatacat ttaaattatc aaatggagtt tccatattcg
     4501 tactttcact atattcatca acattcttag taccttcttc cacacttaaa atacgcttcg
     4561 gacctcctac acagcctcct acacacccca ttccttctat aaatgtagct tctatttctt
     4621 tatttagaac tttctttaga ccgtccatgc actcttttgt tccttcaaat gccttttctt
     4681 caaattttat atttttgtca attcttctga ctgataactt aactgcttta cttacacctc
     4741 ctgttctagc ataaactcta cctgaaaggg acgcctctac tctattactc tcatccatta
     4801 ttgctggatt taactctaat gcatcaaata tttcttgcaa ctctttaaat gtaagcacaa
     4861 aatcaactgc atcttttata tcttctaaca ttgcttcttt cttttttgcc atacaaggac
     4921 ctatgaatac aactttagca tctggattta aaatttttac agctctccca caagcaatca
     4981 taggagaaac ggatggtgat acattctcca atatctgagg aaaactattt tgtatcaaac
     5041 taacccaaat aggacagcaa caactagtta taaagcatcc ttcttcgtcc tttttcatat
     5101 gctcataata atcatatgct tcttttgcag taagcatatc tgctgccaaa gcaacttcta
     5161 tcatatcttc aaatcctatc ttttttaatg cagttcttaa tttaccaggg cttacatctt
     5221 tcccaaactg tcctacaaaa gctggtgcaa ctattgcata tacagggctc tttatatctt
     5281 ttaacaattt tatcattggt ataaattcta ttttatctga aattgcaccc aacttacatc
     5341 ttgatacaca atcaccacaa gcactacata catcttcctc attacaatta atatgcttac
     5401 ttcttatctt acatgactca tcttccttat tattttcatc acaatatttg caagcatctg
     5461 atattacaga tattattggt cttttgactt catctaaatt tacagcttca tgtagtgact
     5521 cttttaaatt tttatctgta tcttctgtca catccaatcc catagttacc ctaattaaat
     5581 ttaccatagt atctttatca aattttccac taaaataatt atttaaatta tcctttaaat
     5641 cttcatttaa aattttatta ggtaagtttt caatatcctc tatatcatca ttccacactg
     5701 cttttacaag ttcgccaaaa agcttcattt tatatatttc tagtatttta gaattgtcat
     5761 ttctagtatt cataaaaaat agtacctcca taaattaaac ttatatatgt ttaatattta
     5821 caaagatact attttttatt tattatttaa ctactattat taatatgatt tatttagctt
     5881 tctcaacaac tctatttcaa tcattatttt cattagatga tagttgaata cggggctttt
     5941 ttcttcatct atattccagt ctagcttatc cacattatat gttttcttaa ggtatttata
     6001 tgtatgctct ttcaaataaa gtttattttc aagtaactct atagactgca tatgaaaatg
     6061 tatttctcta catatcctta aggcattctc aattttttct atattagttt cactcaaact
     6121 ataatccaac tcatcaataa gttttgaata tatttcttct aatttttcta cttcttcttc
     6181 aaatttctct acatttaaat tttcttttct aagaatttta tcttcaataa gtaacattga
     6241 aagttcttct atctttttaa attcatctat aacactattt agataatttg gtctagccac
     6301 ataataatta acaagtactc caaacaaaac acctacagat gtatctaaca ctctatgtaa
     6361 agaataatac acagggtcac tatttcctac acctagatgt atagccagaa aggttacaca
     6421 ggcaactact actgacttta ttttaaacag attgcataaa tatattgagc acattactcc
     6481 tagaccacat aaaaaagggt ctccagcttt aatgatggca aatatgaatc ctattatacc
     6541 acctaaaata gtaccttcca ctctattgat tcctgtcttt aaagaacctt ttaccgtatc
     6601 ttgaacagaa attatacatg ctactagaga ataaaacata ttctctacta gatagttacc
     6661 aagaatcata cataaagtga cactaattcc tgtttttata gtcctcatac ccatcttttg
     6721 aagtttcaaa gttccacctc ctaaaaagca ccttatataa aatacgcatc ctcatattta
     6781 atatgttcct agtatatcac aattttttaa atttacccat taatcatatt tttttatcaa
     6841 ttctttctta atttagacaa taaaaaaact gctttaaagc agttttttat cctatctcaa
     6901 ccattttacc ctcaattgca aaaattatct tctcacatac atttgttata tggtctccaa
     6961 ttctttcaag gtatcttcct ataagtagaa gttttactcc ttggtctata gtatttgaat
     7021 tttcatgcat tatttttaaa caatcaattt gtacttgttc atacaatcca tctacaatat
     7081 catctttagt tccaacatca taagctatac ttgcatcaaa taagattaaa gcttctctag
     7141 tctgagacat catagactta cattcttttg ccatcttagg aatatctatc agctctttta
     7201 tataagcctc tccacctatc ttcaacattt ctctagctat atttgctgca taatctccta
     7261 ttctttccaa ctctatcgat atattaccta atgaataaat aaatcttaag tcccttgcca
     7321 ttggttgctt taatactatt agctctatac tcttatctct tatttcttct cttaatttat
     7381 ttatttcttc atcatcttct actacttttc ttgcaccctc taaatccttc tctatcatac
     7441 aagtcacaga cttttctatt gcttcttcac atttttccat cataagtact gtttgttctt
     7501 ttaaagtatc aatacttata tctaaactat tattgaccat tgtctaactc ctttatagtt
     7561 atatatttta accaaatcta cctgttatat agtcttctgt tcttttatct acaggagtgg
     7621 taaacatagt ttttgtatcg ctaaattcta taacctctcc atttaagaaa aaagctgttt
     7681 catctgatat acgagcagct tgttgcatat tatgtgtaac tatgactatt gtgtagtctt
     7741 tttttaaatc ctctataagt tcctctactt ttaatgttga aattgggtct aaagcagatg
     7801 taggttcatc cataagaata acttctggtc tcatagcaat tgctcttgct atacatatac
     7861 gctgttgttg accaccagaa agtcctagtg cagatgattt aagcctgtct ttaacctcat
     7921 cccaaatagc tgcacctttt aagctttctt ctacaatttt atctaattgc ttcttatctc
     7981 ttagtccatg tgtacgagga ccatatgcca cattatcata tatactcata ggaaatggat
     8041 ttggtttttg aaataccatt cctactttag tacgaagatt tataacatca tctgaggtat
     8101 atatgtcctc tccatcaaca gaaatgtttc ctttaattgt tacatcttcg attaagtcat
     8161 tcattctatt taacgttctt ataaaagttg attttccgca tccagatgga cctattaaag
     8221 cagtaacctt attctctttt atatccatat ttattttctt taatgcttgc ttatcaccat
     8281 aaaataaatc caaatctttt acactcattt ttatcttatc gattaattcc attattcaca
     8341 cctcttaaat ttaataatta gctttattta atttcttagc aacaaatctt gctagcatgt
     8401 ttagtgctaa tacaattact attaaaacaa ttcctatact ggctgctgtt gctatatctc
     8461 cactctcttt agtcagtaag tatgagtgta cagttaatgt tcttgcacta tcaaatattc
     8521 cacctggcat cttggctaca gtacctgctg taagtaatat tgcagctgat tctccaatta
     8581 tacgaccaac tgataagatt actccagata agattcctgg tattgcactt ggaagtacta
     8641 ccttatataa agtttgaaat tttgtagctc ctaatgccaa tgatgattct ctataagctt
     8701 gaggaactgt ttttaaagct tcttctgttg tacgaattat tactggtaat attattattg
     8761 caactgtaag tgaacctgca actatggaat atcccatttt taatgtaact acaaagaaaa
     8821 ttcctccaaa taatccatat actatagatg gtatccctga taaactttca gttgcaaatc
     8881 ttatagctgt aactgctttt ccttttttag catattcttg taaatatata gcagccaata
     8941 ttcctatagg agttgctaca actatagaaa ctactactgt atataatgtt gtaattatca
     9001 ttggaagtat accaccatca cctgatgctg aataattgct aaataagaaa ttaaaattca
     9061 tgttgccaat tccctttata aatataaatc ccactataat gactaatgat actattgtaa
     9121 atagtgctga tagatatact aatgacttca atagattctc tttgaacttt ctcatcttaa
     9181 tttaccgcct tatttgaaag tttatttaaa actaagttta aaattaatat aaatgtaaac
     9241 aatactactc cagttgcaaa taacatttct tgatgtgtac caaatgcgta tcccatttca
     9301 agtgctatat tagttgtaag tggtcttaca ctatctgtta ttgcagttgg cataactggt
     9361 gagtttcctg caacaagtat tactgccatt gtttcaccta aagctcttcc tactcctaat
     9421 actactgcag ctaatactcc agatttcgca gcaggtagta caactttgaa tattgtttct
     9481 atttttgatg caccaagtgc taatgaacct tctttgtatg cttttggtac tgctcttatt
     9541 gctgtttctg atacagatat tattgttggt aacatcatta ctgctaatac tattactatt
     9601 gctaacaaac tttgaccttt tggaagatta aatatacttt gtatagttgg aactatcact
     9661 gcaagaccaa atacaccata taatactgaa ggtatacctg ctaaaagttc aactgctggt
     9721 gacattattt ttgctaatct tttaggtgct acttctgcta taaatacaga ggttaatata
     9781 ccaacaggta ctcctatcac aagtgctcca agtgttgcaa atattgatgc tactatcata
     9841 gatgatattc caaatttatt ggcagaagga acccaatctg caccagttaa aaagtctata
     9901 aatgaatatc cttcaaatat aaatggtctt aagcctttat aaaatacgaa tcctattatc
     9961 aaaagtaaac ttgcaactgc tactaaagca ctaagtagaa aaatattttt tgctactttt
    10021 tcaattacgt attttgtctt attgccatta ttgtcatttt tttctaaacc tactaatcta
    10081 gattttacta tcataatacc ctcccattaa aatatactta tcgattttcg ttctgtattc
    10141 aaaatttaat tttttataat tcgtcttcat caaatttcct gtacttttat tctacttttt
    10201 ttaagttaaa tctatattag gattaagtta agcaagtgta aaattatatt tactttttta
    10261 acattaattt tacttttaaa actatatttg acatttactt tacaatatac tgttgtttct
    10321 ctatattaaa atagcagata tagataataa tacagtttat gtataattta ttacctatat
    10381 ctgctattaa gatttgttaa ctaaatttaa cttttatact acatatataa ttatcgtatc
    10441 ctctagttta tatcactatt atttctatca aattgttatg atacttgatg tttgcaatag
    10501 gtacgaatag ccaaaaatat aaaaataggt atttctatca ctgagaaaaa cagtataatg
    10561 tgtggtgtcc aaaaactcat acctcctaag tgtaagaaat taaatgatgt catttgatat
    10621 aataaactaa tcttaagacc aattccccct ggtggtaata ggtatccaat ccaactaaca
    10681 cctattccca taggaattaa gcaaatagaa aaagcaatca gcatagaagt aagagaatcc
    10741 ttacattttg cagataaaaa taatgttaaa ctaaccattg caagtattga aattagacca
    10801 ccaatagcta taagaatctg tgcttgtagt aaatttatat ttactagact aatgacagaa
    10861 aacaacattt gaaatgaggt tttcaaacat tcagtaccaa aagctaaatc agaaattgcc
    10921 aaatggatag aaatacatac aacaaacatt actataaata tagaaaatag agctaatatt
    10981 ttagtaaacg ctagacgagt atgtccatgt ttcgtacaac gaaggatgct atctgattgt
    11041 gtctgatact caccagaaaa agtaggtgca gtaattgcaa cacatataat taataatata
    11101 aaaatgtaaa atattatata atcgaaagca tctttagaat aacctgggta tatctcaaat
    11161 ggagtatcta cttttctata aagttcttga gctttcattt gtgcactgga agtttgtcgt
    11221 tcaatcttca taatatcatt taaatgagtt ttgcattgtt cataaaaaga atctaaatca
    11281 tttgtattta tttctgataa ttctgttgca aatcctgttt ctgaattagc aaatgcttca
    11341 ggaagtctat tcaacaatgg gcgtattggt aaaatacttt tagaataaat atctagtggg
    11401 aaatcttcac tacctacttt accatacttt tcttctgttt ttttccatat atttaaagca
    11461 tgctttaatt tttttggtgt tacaactcca ctactaattt cactattatt agcataaatt
    11521 gtttttttat atttaatagc atctaaacca ctcaattcaa ctaccttgcc attattatct
    11581 aaataattga taccctcgta agtaattgga agaaaagcca tcaatgcaga taaaaccaat
    11641 gccaacaata ataaaattaa tgtacgacgt gagtgcaaca ctcgtttaat ttccaaataa
    11701 aaaatacgca tttatttccc ctccttacta taaactgctt ctggaaataa ccataaatat
    11761 aaatcttcta gtcgtggttc aacagcaata gagttatcaa tttgtgactt ttctgccaag
    11821 taacgaatag atacttgatt gtttttttca ctacgctgat tgattatacg taaattcatt
    11881 tcatattttg aaatatctct atttgatata agacattccc atactttgcc ctctactttc
    11941 ttaaccagtt tatcggttgt tcccacatca agaatttttc cacttttcat aatagcattt
    12001 tgagttgata tgtactcaat atcagataca atgtgagtag atattaggac aacacgatta
    12061 tgagaaaatt ctgaaataaa attacgaaat ctcactcgct caccagggtc aagccctgct
    12121 gttggttcat ccattataag gattttaggg tcatttaaca tggcttgtgc aattcccact
    12181 cgtcgtttca ttccaccaga taatttaata atctttttat tttttacatc agataatgta
    12241 agaatttcca atagattatt aatttttcct tttacttctc ttggtggtac atctttcaat
    12301 gctgctatat attctaaata atcttttaca gaaaaatcct gtgggaaacc aaattcttgt
    12361 ggtaaaaatc ccagtaaatt tctgtactca gcacccatta tataaatatt ttttccatta
    12421 tacagaattt tgccattggt aggtttcata atgtctgtaa taattctcat caaggtagtt
    12481 ttacctgctc cgtttgctcc tagaaatccc cacacacctg gtgttaaggt aaggcaaaca
    12541 tcttctacag caattttatc cttaaatttt tttgtcacat gttcaatttt taattccata
    12601 tttgaaactc ctctctttat ctacttacct gcaaaaacaa aagtgtcttg cttagtattt
    12661 attttactaa acaaagacac tcataatttt aatattaaat agataatttc ttaagaaaat
    12721 cctaatattt gatttatatt ttactaaata gatgtaggaa gcgatatagt aaatgttgtg
    12781 acttcatctt tactttgaac agtaatatca ccaccatgca aatttataat ttccttagct
    12841 atagaaagcc ctaaacctgc accacccata tcagattttc tagcttcatc taaacgattg
    12901 aacttttcaa atatagcaga aagttgttct tctggaattg tctgtccata atttctaaaa
    12961 gcaatatcta ccttgctgtc tttatgaacc gctgagataa taatttctgt attaggatag
    13021 ctgtaagcaa tagcattttt caagatatta ttaaacactc ttgcaagttt ttcagaatct
    13081 gcttttattg ttaaactttc atttgctttt agaatggctc tatttccttt ttcagacagc
    13141 aatgggtaaa attcatcctt taattgaagt aacatataat ataggtctac tttttcgaaa
    13201 ttaattttga tttgattaga attatatcgt gttatttcaa aaagttcatt gataagtttt
    13261 tctaaatatt gtgctttgtc aagtgcaact tcagtatact tagatttttg agaattatcc
    13321 atattaggta tttcttttaa taagctcaaa tacccaataa ctgaagtaag tggtgtcctt
    13381 atatcatgtg ctagatacat cacaaggtta ttctttttat tttcagcctc tttcactgct
    13441 tgttgactta acaaaacaga catcttaatt tggttcattt gttgctctat ttcctttaat
    13501 ggcttagata gttcaaccaa ttcatcattt tgttgataaa tagtatgtgt tgcattgatg
    13561 atttctctta aataattcca tggttttttc caataaaaat tgaaaatagt gatatatcca
    13621 attataaaaa ccaaaaaaaa taatacatct cttctcaact taagccattt aacaagagga
    13681 tttgaaaaat ttgaaaacgt ataatcagcc ataaacatta aaaccaatac tatacctgta
    13741 tagatagcta acattatata taatttatac tttaaatacg cctctgtatt tttttctctt
    13801 tttataaatt tatatttatc tcttagtttc ctataagtaa aattattaat ctttttcata
    13861 atactacatc ccttcttgac aagaaaaata ggtgtcaaat cttgtaacca acaccccaaa
    13921 ttgtttttat atattttgct tcatctacag tatcacctaa tttttcacga agatggcgaa
    13981 tatgaactgt tatagtattg ttacttttat tgtaatattc atctttccag atagcatgaa
    14041 aaagattttc tgaatttacc acagagcctt tatttttcaa taatacatgt agaatagaaa
    14101 actctgttgg agtaagtgaa agagctttac catttaaatg gcattcatat gtttgggtat
    14161 tcaattccag acctaaaaaa gaaaaaatat gtgaaagttc ttcaccagat tgtaaacttc
    14221 cactgtattt cttatatctt ctaatctgtg ccttcactct agcaacaagt tccaaaggtc
    14281 ggaaaggttt agtaacataa tcatctgcac ctaaagttaa gcctgtaatc ttgtctgttt
    14341 cttcatcctt agctgttaac ataatgattg gataagtata tttttctcgg attttttggc
    14401 ataaagaaaa tccatttact cctggcaaca taacatctaa aattgcaagg tcaaattcat
    14461 ttaaattaat gcagtttagt gcatcaagac cagaataaaa tttataaaca atatagtttt
    14521 catttgtaag gtagacttct attaaatcag ctatatcacg ttcgtcatct acaactaaaa
    14581 tttttccact catagacttt aaacatcctt tctatatata tttatatata ttatactaaa
    14641 tttaattttt ttatatgatt ttttacatta gaatttatta agaaattctt aatcttatta
    14701 tgtaactatt ttattattta acatatatta aagataaaaa tgaatatata tagatttaga
    14761 tatacaattt atctatcttt tcaaatataa tctataaaat aatagttctg ttacgcattt
    14821 tttcatataa taaaactgac tgtaatttaa ctggcaaagt ctagaagaat aggctttatc
    14881 aattaaatta cagtcagcta ttttataata atttagttta gatatcgtta gcatttcaca
    14941 tttataacaa ttattaatga gattaatata tttattattc tttattaaaa taatatcctg
    15001 ctcctcttat tgttataatt ttctgaggat ttttataatc tttctctatt ttctttctta
    15061 atccattcac tgcaactacc acactatagt tatcctctaa gtactcattc atccatacat
    15121 gttcatatat ctctttagta gtcagagtct tttcaggatt ctctataaaa aagtttaaca
    15181 gcttattttc tgttggagtc aattttatta ttttgtcttc taactttatt actccactag
    15241 ttctattata ttcctctaat tttgtattat tttcattatt atttacatat gagttcattc
    15301 ttcttaaaat agcttttact tttgcatata atatttctaa atttagaggc tttattaagt
    15361 aatcatctcc gccagaattt aaagcattta aaatgctttg ttcttcattt atataagtca
    15421 tataaacaat tggacaagta gttacatttc taattttttt acaaagagtc catccatcac
    15481 catcagttag aattatttct aaaaatatta aatcatattt attgctaaat atttttccaa
    15541 tagcttcttc acaattatat gctaaatcta tacttatttc tttttcctcc agaaatcctt
    15601 ttagcttctg gcaattatcc ttgtctccat ctattatcaa tatcttagtt tgcattataa
    15661 tcactcccaa atatcattat tcttttatat taaataagct attattataa aaaacataga
    15721 cttaatttta ttgtaaaaac agttcctttt cctagttctg aatctacaaa gatttctccg
    15781 ccatgctctc ttataatgga tttgacaata tataacccta gcccttgccc ttttggctca
    15841 ttattatctc tttctgtaaa gaatctgtca aatacctttg gtatattttc tggaggtata
    15901 ccttttccat tatcagaaac tttaataatt gcaaatccat cttctttatc cattgataaa
    15961 attatctttc catcaaatgg agtaaatgtc aaagcattat aaattaaatt ctcaaatgct
    16021 cttgtaagca tctctttatt tatctttgca tatatttttt tctccaatat gtaagtttca
    16081 aagtttacac cacatgcttg tgtatctggc aagttatttt catatatctc tttaataaaa
    16141 tcatttagac atattttttc actaatacca atgcttttat cttgtgatgt aaattcttgc
    16201 aaactacgta ctctattttg tacttcattt gacttttgat ttatagcata aagataggtt
    16261 cttgtctctt catctacatg aatattacca acctttatca agtctataaa tgattgtata
    16321 gctgaaacag gtgcctttaa atcatgtgcc acatctgcca agaatttttt acgttcattt
    16381 aacaatgttg taagttcatt agttctcact tcaacttcat gttcaagatt ccttgtaagt
    16441 ttttcattct cttttattat tattctattc ctgtttatca tcataagaga aaataaaata
    16501 acaattataa atccacaata ttctgtttgc caccctgtaa atataggctc aaatctatta
    16561 ctagtaactg catcaaataa tatgctaatt ccaaaaacag tgtttactga aactaaaata
    16621 taagaactta tatgctcaga gcatgtcttt ttatatattg aaaccagcga agacattaat
    16681 atatatataa atacaatata tttatatatg tctattaatt tcccatatat gttgacaaag
    16741 tacatatcat atggaagaag aataattggt atcactatag atatgatact cattgtaatt
    16801 cctaatggta aaattaaaaa tctatacact ttatcttctt ctattttgga tatacttcca
    16861 tttatacaaa taatacataa aactaccata aaataggacg aatcttcaac cgcatatgta
    16921 aagcttacca aaggcagtcc taacaaatgt acaaagggat aggatacatg gatggcaaag
    16981 aaaacacaca tacatccaaa atataagtaa agtttacttt tgtttgaaag aaaccataca
    17041 gacaacgaaa atatagctat tgcaaaagtt gtgaagcaaa ttatactata aaaaagtaat
    17101 cgataaaaca tcatattact tatgtccttt gtttctccta atgcaggcgg ataatacaaa
    17161 ccactataat aatgtgaaaa attcgataca ttgattataa tttctgtatc tttctttgca
    17221 ataaaactta ctactgaatt tttaattttg ggtctatatt tttctgttcc tacatctcct
    17281 aatttagaaa ctatttctcc atttatccat acttcacttg cggaaaaaat ctcaggaagc
    17341 tctaaactta gtagcttatc ctcaccttca ttttttattc tcatacgata agtagcattc
    17401 ccatatggtg actgattccc ttttctcata gaaaaatctg gatattgacc tataaaaaca
    17461 tattctggtt ttatatcttg ctctgtaaaa tcattcggtt taaatatttt atgattataa
    17521 aactcccagt catctatcag aaagacaagt ttacctttag tgaaagtatc attatctata
    17581 gttaaaattc cattacttgc tctaatagac ttatatgtat atttattatc atatttatac
    17641 agaagattta atcctgctat tgccaacaga gagactatca atacaatgag agttcctttt
    17701 agtatattat actttttcat tgcatcaacc acatctcttt catagtagta ttaaataaaa
    17761 caagaactat ttattaatta cagtatctca cttatggtac aaacttatat ccttttcccc
    17821 atacggtttg aatgtattga tgctccctgc ttaaagaatt aatttttttt ctcatctgtg
    17881 caatatgaac ctgcactgta tgttgatctc caatatcagg cattttccaa acttggttat
    17941 atatctctat cttcgaaaat acttgttctt tgttttcagc tagaaacaat aatatttcaa
    18001 attccatagt agttaaatct atactttctc catcaaaagt tacttttctt gaatttacat
    18061 ttattactaa cgctgaaaac tttaacacat taggaactga tgtaatattt ttgtactgcc
    18121 tcatacgagc atatattcta agttcaagtt cttttaaact ataaggtttt gttatataat
    18181 catccccccc agctaaaaat ccccgaactt tttcttcttc atgctcataa ttagataaaa
    18241 atattatagg tatgttaacc ttttctttta tatcattgca cattttatat ccatctatat
    18301 ctggcaatga aatatccaaa attatacagt ctaaaactat acgctctact atatccatag
    18361 catctttagc agtctctgat gtatatacat taaaacctat attagaaaaa taaatttgat
    18421 ttatatttaa aatatcaaca tcatcatcta caaccagtaa attatacatt acaccactcc
    18481 attcaaaggt attttaatca taatctcaag ttttataatt attacaaatt ataatgtttt
    18541 attttcatat ataactagta ttattatttt ataatatctt tacaaactag actat
//
LOCUS       DAELXX010000080        18535 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.080, whole genome shotgun sequence.
ACCESSION   DAELXX010000080 DAELXX010000000
VERSION     DAELXX010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18535
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.080"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..888
                     /locus_tag="KPH29_002596"
     CDS             <1..888
                     /locus_tag="KPH29_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468182.1"
                     /translation="YKIDCDVILVNLFELIINNCIFSTILGNEVINLEISKYEYEFLE
                     KQFKNMYNNITEDKVIGSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKK
                     ITLLLMEAIEVLIKRLSIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKN
                     EHLEDYIVDDESKLDDESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECL
                     FGEEYLMLFASLSELELAILGSVVFYEDIRMREINILEFMLNEKTETTLWKTEYIKFM
                     KRQSEDKIYSIEEHMNKIN"
     gene            972..2339
                     /locus_tag="KPH29_002597"
     CDS             972..2339
                     /locus_tag="KPH29_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG1468183.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTVMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKARDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMNTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            2374..2598
                     /locus_tag="KPH29_002598"
     CDS             2374..2598
                     /locus_tag="KPH29_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HBG1468184.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGATMTIKAIIENVLVSEVPGVTQVLGV"
     gene            complement(3048..3446)
                     /locus_tag="KPH29_002599"
     CDS             complement(3048..3446)
                     /locus_tag="KPH29_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HBG1468185.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMVG"
     gene            complement(3449..3850)
                     /locus_tag="KPH29_002600"
     CDS             complement(3449..3850)
                     /locus_tag="KPH29_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG1468186.1"
                     /translation="MLLKELYSKTMYTVEENFKENGLTHQQIIVIKLVAHNQELTISQ
                     LCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTTGFEFATNFKIKMQE
                     SFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            4209..5195
                     /locus_tag="KPH29_002601"
     CDS             4209..5195
                     /locus_tag="KPH29_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1468187.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLNEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            5179..6090
                     /locus_tag="KPH29_002602"
     CDS             5179..6090
                     /locus_tag="KPH29_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1468188.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIRNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            6203..7771
                     /locus_tag="KPH29_002603"
     CDS             6203..7771
                     /locus_tag="KPH29_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG1468189.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHVLEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGIFWNIADWTIE"
     gene            7846..8871
                     /locus_tag="KPH29_002604"
     CDS             7846..8871
                     /locus_tag="KPH29_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1468190.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDEYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            8864..9808
                     /locus_tag="KPH29_002605"
     CDS             8864..9808
                     /locus_tag="KPH29_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009527644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1468191.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKSLSWIEVSDGHFVYSSKSEINKYQQNLKVV"
     gene            10167..11825
                     /locus_tag="KPH29_002606"
     CDS             10167..11825
                     /locus_tag="KPH29_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG1468192.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERDSKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASNGKSSW"
     gene            11806..12822
                     /locus_tag="KPH29_002607"
     CDS             11806..12822
                     /locus_tag="KPH29_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG1468193.1"
                     /translation="MVKAVGDIIGKCSAISFDMKLDKSYDEVIVDFKNLINNIDNKDV
                     LLFTDMGSLNSFDEIIKKEKKCEVRVIPMVTTLTVLEAVQKANMGLPLNDVYNSITNT
                     RKYYFGTNEIQNKENLSKTIIIASHVSEGVDNKTRKILEEKMSRYLDGIDIISVPYKT
                     EKDLSLNITKLKESSNIVAVINEQRINIRGIDYISKKDIDKDENINKLKNIIKISIGY
                     DDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLVFNIEKKYDKVIGLMMHLAFMVDGLI
                     GNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDKKYNIEINEKECYQILLILEYAEIIE
                     KDYQ"
     gene            13037..13666
                     /locus_tag="KPH29_002608"
     CDS             13037..13666
                     /locus_tag="KPH29_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468194.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            13801..14127
                     /locus_tag="KPH29_002609"
     CDS             13801..14127
                     /locus_tag="KPH29_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG1468195.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            14143..15432
                     /locus_tag="KPH29_002610"
     CDS             14143..15432
                     /locus_tag="KPH29_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG1468196.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            15456..15785
                     /locus_tag="KPH29_002611"
     CDS             15456..15785
                     /locus_tag="KPH29_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG1468197.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            15933..17261
                     /locus_tag="KPH29_002612"
     CDS             15933..17261
                     /locus_tag="KPH29_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG1468198.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            17578..18438
                     /locus_tag="KPH29_002613"
     CDS             17578..18438
                     /locus_tag="KPH29_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HBG1468199.1"
                     /translation="MAMKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKE
                     VPSKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDI
                     EEVEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCD
                     KIIKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLN
                     TIKEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEE
                     EEEKYRRIFK"
BASE COUNT         7447 a         1977 c         3296 g         5815 t
ORIGIN      
        1 tataaaatag attgtgatgt tatacttgta aatttatttg aacttattat aaataattgt
       61 attttttcta ctattttagg aaatgaagtg attaatttag aaatttcaaa atatgagtat
      121 gaatttttag aaaaacaatt taaaaatatg tacaataata ttactgaaga taaagttata
      181 ggctctgaag aaagtaatat aattgaattg attgaagtag atgatagttt taacactgaa
      241 gtagatagta atttggatta taatgatttt gaagaaatta acagagaaat tagaataaat
      301 aaaaaaataa ctttactact tatggaagca attgaagtat taataaaaag attaagcata
      361 gaagatgaag atgtaataag atatattaaa aaatatgaaa agatatttat tgaatctgtg
      421 ctaagctcaa taaaaagtaa cacttttaaa agtatggtaa ctgtgagtaa aaataaaaat
      481 gaacacttag aagactatat tgttgatgat gaaagtaaat tggatgatga atcgtttaga
      541 gagatattca atgagatatt agatagtaca tctataattg aaaaaataag aataataaag
      601 gaaaatatca atgctaaaaa agactttatc gatattcttg aatctgaatg tttatttgga
      661 gaagagtatt taatgttgtt tgcatcatta agtgaattag aattggcaat attgggtagt
      721 gtagtatttt atgaagatat tagaatgaga gaaattaata tattagaatt tatgttaaat
      781 gagaaaactg aaactacttt atggaagaca gagtacatta aatttatgaa gaggcaaagt
      841 gaagataaga tatactccat agaagagcat atgaataaaa ttaattaaca aaaaattgtg
      901 aataaataga atgttagtat ataatataat ctgtaaagca tatcaaaaat acatatattt
      961 ggaggaaaat tatgaaagag tatatcataa ataatgttga agaaataaga gaagaattaa
     1021 ttgatttatc taagaaaata tgggaaaatc cagaattagc ctttgaagaa aagtatgcaa
     1081 gtagcattca aaaagaatac ctaaagtcaa aaggatttaa aattgaagaa gtagaaaatt
     1141 tacctactgg atttatagca agttttggag agggtaaacc tgtgattggc atacttggag
     1201 aatatgatgc tttaccagag ctatcacaat gtgtgagtgc agaaagaaag ccattggtag
     1261 aaggaaaagc tggacatgga tgtgggcata acctgttggg tgtagcaggt gtaggagcag
     1321 ttgtttctat aaaaaaatta atagaagaaa ataaatttaa tggaacaata aaatattttg
     1381 gttgtcctgc tgaagaagaa ggtggaggaa agacagttat gtgtataaat ggctgttttg
     1441 atgatgtaga ttgtgcattt acatggcatc cttttgatat aaatgcacct tggagaggtg
     1501 gaagtctggc aaacttatct gtaaaattta aatttaaagg gataacagcc catgcagctc
     1561 aagctcctca taatggtaga agcgcacttg atgctgttga aattatgaat gttggagcta
     1621 attatttgag agaacatgtt attgactcta taagaatgca ctatgttata actaatggtg
     1681 gtggaagacc taatgtagtt cctggatttg ctgaaagttg gtactttatt agaggtaaaa
     1741 aagcaaggga tgcagaacat gttcttgata gattaataaa agtagctcaa ggagcagcaa
     1801 tgatgacaga aactgaaatg gagtataagg ttacagatgg aatatatgac tatataccaa
     1861 accaatgttt gactgattta gtttatgaca atatggtttt tgttggttgc cctaaaaata
     1921 ctcctgaaga agaagaattt gcaaaaaaat tgtgtgatac tcttactaga gaagaaagat
     1981 taggtgtagc aacatcatta caaaatgata aatctattgt agaaagttat atacacaagg
     2041 aaatagttga cggtgacaaa gataaaggac tagctggttc tactgatgtt ggagatgtaa
     2101 gttatgtaat accagtggct caatttgcaa tggcagcttg gcctgttgga attgcaagtc
     2161 atacatggca gtcatgttca tcagcaggtt caaatatagg attttctgct atgataaatt
     2221 cagctaaagt actggcttgt agtgcttatg atgtatttat gaatactaaa attattgatg
     2281 aagcaaaaat tgaatttgat aaatcattag atggacaaaa atttaaacca ttggtataat
     2341 tttggtaata aaatatttct ggggggaata gctatgagag aaaaagttga aaaagtgctt
     2401 gaggaaaaga taaaaccagt attacaaaga gatggtggag atgtagagct tatagatgta
     2461 aatgaaaatg gtgtggtttt agttagacta caaggggcat gtagtgggtg tccaggagcg
     2521 actatgacaa tcaaagctat tattgaaaat gttttggtga gtgaagttcc aggagtaact
     2581 caagtattag gtgtttaata gttagcgttt attaaatgta tgttatacat aagtatttaa
     2641 taaagaagta aaaaaaatag tgaagcaata tatatgaaaa agtatgataa agataataaa
     2701 acattataga aataatgaaa tatttcatat gtatatgaag aaataagaaa aaatagtgaa
     2761 gcaatatata tatgaaaaag tatgataaag ataataaaac atttatagaa ataatggaat
     2821 atttcatata tacatatgaa aaaatagtaa aacaatatat atatgaaaaa gtgtgagaaa
     2881 ataataaaat actatagaaa caattaaata ttctatatag gtattaagaa acatgaaaga
     2941 aataatggag tattatagat aaatatgtaa aagtgtgaaa gaaggatgta gataaatttg
     3001 tcttatacaa atttacctac agtatttctt acacactttt tattttacta tccaaccata
     3061 ccaataaaca tacctataaa gtatggaact aaccatatag tccatacaac tatactaaac
     3121 ttatgaaaga attttttctt ttcctcatca tctttgtata ggacaaaagt agcccaactt
     3181 gcatgaaaaa gcataagcac aattgcaaga agacctgtaa tactatgaag gttttgtgat
     3241 aaagcagatt ttacttcaaa agtatggctg ttggctatgt tactcattgt aaatgttcct
     3301 aaagtatcaa atataaaccc aagccaaaat ataattacat gtttcttttt taaaattttt
     3361 gcctttcttt ctccaaatac acctatagtg tagaaaatta aagctgatgt tataaatact
     3421 attgcaagta ttaatttata attcataatt atctctcctt tacttttgcc aatatatttc
     3481 taagattttt tactaagtca ctcaattcgt tttcatcaca atttttaaaa atatcatcaa
     3541 aactttcttg cattttaatt ttaaaatttg tggcaaattc aaatcctgtt gttgtaaact
     3601 taacgtatgt atttctttta tcattagatt tcttgaattt ttctatatat ccaatctgtt
     3661 ctaatctgct gattatgcct gaaaccgtac ctttagctaa agacatttca tcacatagtt
     3721 gtgatattgt aagttcttga ttatgagcta ccagttttat aactataatc tgttggtgtg
     3781 ttaatccatt ttctttaaaa ttttcttcta ctgtgtacat agttttagaa taaagttctt
     3841 ttaagagcat ggctattcta aaacagtcat aattactttt caataaaatc acctcttaaa
     3901 tggtttgcat acaaactaat ataatatact tttcttatat agtcaaccta aaattttata
     3961 attttataga aaataatgaa gaatagaaca taaaaatttt attttaggta taaataaaat
     4021 aatttgatga aattttaaca atttttaaaa agtttgttta cacagttaat aaatgatgct
     4081 aaaattaact catagataaa tatagaaaat ttaattttgt ttaatgataa tggaaccacg
     4141 aaggttttaa cgttacttta cgtttgcttt tatgtggttt ttttattgca acaaaggggg
     4201 gttggggttt gttaaaggat atactgaaaa agttgttgca gtttatttta gtaatgtttt
     4261 tattatcttt tattgttttt tacatggcaa gacttgctcc aggagaccca ttaatctctt
     4321 actatgggga tggagtagaa agaatgagta cacaagaaaa agaaaatgct atgaaaaaac
     4381 ttggtttgaa tgaaccaata tacagtcaat atataaaatg gatttcaaat gcatcaaaag
     4441 gggaatttgg aatttcattt aaatataaac aaaatgttac ttctgttata aatgatgtct
     4501 atataaacac tataatatta ggtggttcag ggtatatact tacctttgtg ctagccttat
     4561 tgttaggaat attttgtaca ttacatgaag ataagctcat tgatagaatc atatgcaaat
     4621 taggaacaat aaccaactgt attccatcat tttgggttgc cttagtactc atacttatat
     4681 ttagtataaa cttaagtata cttccaagta gtggtgctta ttcaatggga gaagagtcca
     4741 gtatattaag taggatatca catctaatct tacctcttac agtacttata ttaagccatc
     4801 tatggtatta cacatatatg ataagaaata aattactaga ggaaataaga gaagattatg
     4861 tgcttttatg taaagcaaaa ggtttaaata atagaactat tgtttttaag cattgcctga
     4921 gaaatataat gccatcatat attagtataa tggccatttc gatacctcat atactaggag
     4981 gaacttatgt agttgaaaaa gttttttctt atcctgggtt aggaacactt tgttttgaaa
     5041 gtgcaaagta tcatgattat aatatgttat tagttttatg cttaataaca ggggctttag
     5101 tagtatttgg gaatatgtta gctcaaatta taaacaataa aatagaccct agaatgaaat
     5161 atgatagagg tgatactaat gaagccacaa tatagtgatt ttgaaattgt aggagaaaat
     5221 tatattttag tagcagaaga tgaacatata gaaacaaaga aaaccttatc taaaaaaatt
     5281 aaagaattac catatatatc aattattatt ttatcaatca tagtcattgg aagtgtattc
     5341 tcatctttta taatgacaca tgaaccaaca tacatggatt tagcaagttc taatttagct
     5401 ccaaataaaa cttttttatt tggaacagac tcaatgggta gagatatata ttctatgata
     5461 tggtatggag gaaaaatatc tttatttata ggactatttt ccactgtaat atcaactgtt
     5521 ataggaattg tttatggtag tataagtggt tcagcatctg aactcgtaga tgatgcaatg
     5581 atgaggttta cagaaatcat acttagtata ccttcaatat taattataat atttgtacaa
     5641 gctatacttg gaaattcaaa tccagtatct atgtccatag taatagggat aacaagttgg
     5701 atgaatatat ctaagatagt gagaacagaa gttagacaaa taagaaatag cgaatacata
     5761 ctagcagcta agagcatggg aggaggattc ttctatgtat taaagcaaca tttacttcca
     5821 aattttgtgg catcaataat gttcatggtt gtaacaaaca taggcgcagc tattggtact
     5881 gaatcgacac ttagcttttt aggaattggt ttaccaatag aaattgtttc ttggggaagt
     5941 atgttatctt tgtcagagga agcattactt tcaaatagat ggtggattat actgattccg
     6001 ggtatatttt tggttaccac attggtgtgt ataacaaata taggtaatta tataagaaag
     6061 aataataata aaaaatctag taatttataa atcagcattt gagtactaaa taagcaaata
     6121 ctgaggtatt tgaaatatct atttaaataa atggggtcaa agctttgaaa aatattttat
     6181 aaaaagcaaa agggggagca tcatgaaatt aaagaagtta aaagttttaa gtttagtaat
     6241 gatacttagt ctaatggcag gttgttctag tggaggagac aaagataaga aggcagatac
     6301 gcctaaagat ggaaaggtac ttgtttatgg aagtaatgac tatacaagta taaatcctgc
     6361 actatatgaa catggagaaa taaattcatt aatatttaat ggattaacag ctcatgatga
     6421 aaataataag gtggttcctt gtctagcaaa ggactggaaa tttgatgagg caacaaatac
     6481 atatactttt aatttaagag atgatgttaa gtggcatgat ggagagaaat ttacggcaaa
     6541 tgatgttaag tttacaatag aaactataat gaatccagat aatgcttctg agatagcatc
     6601 aaattatgaa gatataacaa aaattgatgt ggttaatgat aatactataa aaattacttt
     6661 aaaagctcca aatacagcaa tgcttgatta tttaacagta ggagtattac caaaacatgt
     6721 tttggaaggt aaggatatag ctactgatga atttaatcaa aaaccaatag gtactggacc
     6781 atttaaactt gaaaaatggg acaaaggtca aagtataaca cttgttaaga atagtgatta
     6841 ttttgtaaaa gaaccaggat tagataaagt agtctttaag attgttccag atgataaggc
     6901 taaggcaatg caattaaaat caggagaatt agacttagct caaataactc ctaaagatat
     6961 gtctaacttt gaaaaagacg aaaagaattt taaagtaaat ataatgaaaa cagccgacta
     7021 tagaggtata ttatacaatt ttaattctaa attctttaaa gataaaaaag ctaaaggatt
     7081 accaaatgct ttaagctatg caatagatag aaaagcaata gttgacagtg tattattagg
     7141 acatggtgta ccagcatatt cacctttaca aatgggacca tataataatc ctgatataga
     7201 aaagtttgaa tataatccag aaaaagctaa acaagaaatt gaaaagctag gttggaaatt
     7261 aggttctgat ggaatctatg aaaaagaagg tactaagtta gctttcgaga taacagcagg
     7321 tgagagtgac caagttagag ttgatatggc taaaatatgt gcacaacagt taaaagaaat
     7381 tggtgttgat gctaaagctg ttgttgtaac tgaaacagat tgggctaatc aagatgctca
     7441 tttaataggt tggggaagtc catttgaccc agatgaccat acatataaag tatttggaac
     7501 agataaaggt gcaaattaca gtgcttactc taatcctact atagataaaa tacttcaaaa
     7561 agcaagagaa acagaagata aagatgaaaa attaaagtta tataaacaat tccaagtaga
     7621 aatgactaag gatatgcctt atacatttat tgcttatata gatgcaatat atgttggaaa
     7681 accaaacata aaaggattaa cacctgatac agttttaggt catcatggtg taggtatatt
     7741 ctggaacata gctgactgga caattgaata atactgaaat taattagaaa ccttagggat
     7801 aaaaatatat ccctaaggtt tgaatagcat aggggttgat ggtttttgga acatttattg
     7861 gaggttaata atctatctgt aagttttaaa gtagaagaag gagaagttca ggctgtaaga
     7921 aatgtatctt ttaatctaaa aaaaggtgag acattggcta tagttggaga atctggttgt
     7981 gggaaatctg tactgtgtaa gagtttgatg aggatactcc catacaatgg atatattaaa
     8041 aatggagagg ttttacttaa atcaagtgat ttggttaaga agtctgaaaa agagatggaa
     8101 gatataagag gaaagaatat atcaatgata tttcaagacc ctatgacatc tctcaatcca
     8161 actatatcaa taggaaagca aattgcagaa gctgtaatca ttcatcaagg tataagtaaa
     8221 agtgaagcaa aaaagagagc catagagctt attgaattgg ttggaattga taatcctgag
     8281 aaaagattta aacaatttcc acatcatttt tcaggtggaa tgagacaacg tatagtaata
     8341 gctatagcac ttgcctgtaa tccagatgta ttgattgctg atgaacctac aactgcttta
     8401 gatgtaacta tacaagctca aataattgat ttaataaagg atttacaaca taaaataggt
     8461 ttgtcaataa tatttataac tcatgattta ggagtagttg caactatagc tgatagaata
     8521 gcagttatgt atgcaggaaa gattgtagaa ataggaactg tagaggatat attttatgac
     8581 cctagacatc catatacttg ggggttgttg ggttcattac ctactttaga ttcacaggat
     8641 gaatatttat ataatatacc tggaatgcct ccaaatttat tgaacccacc aaaaggagat
     8701 gcttttgcaa taagaaataa aaatgcactt aagatagatt atgaaaaaga gccaccaatg
     8761 tttaaaatta atgatacaca tagtgcagca acttggttat tgcacccaga tgcacctgaa
     8821 gtagatgtac cagttagagt aaattgtgga agggtgattt ctaatgaata gagaaaaaat
     8881 actggaaata aaaaacttaa agcaatattt tcatttggac aaaagtacta ctgtaaaagc
     8941 agttgatgat ataagttttg atatatataa gggtgagata tttggattgg taggagagtc
     9001 gggttcagga aaatctacaa ctggtaaaac tataataaga ctacatgaat ctactggtgg
     9061 agaagtaatt tacaaaggaa attgcatatc agataaaaaa acgtacaaat ttataaaaaa
     9121 agatgtaaac aaaagtatgc agattatttt tcaagattct acttcatcat taaatccacg
     9181 tatgacaatt gcagatataa tttcagagcc actaaaaatt caaggtatct gtaaaaataa
     9241 aacagataga atgaataaag tatatgagat gctaaagcta gtaggacttg atagaagtta
     9301 tgcaaataag tatccatctg atttttcagg tggacaaaga cagcgtattg gcatagcacg
     9361 agctctttca gtagacccag aatttataat agctgatgag cctatagctt ctttggatgt
     9421 gtctatacaa gcacaaatag taaatttatt taaaaaattg caacaagaaa agaatttaac
     9481 ttgtttattt atagctcatg atttatctat ggtgagacat attagtgacc gtataggagt
     9541 tatgtataat ggaaaattag tagaattggc agattcaaat gaattgtata ataatcctat
     9601 tcatccatat acaaaatcac tactttctgc aattcctgtt cctgacccta gatatgctaa
     9661 gtcaagaaat agaatagagt ataattctaa tggatatgat tgttcaaatg aaaagtcatt
     9721 aagttggatt gaggtttcag atggacattt tgtatattct tctaaatcag aaataaataa
     9781 atatcaacaa aatttaaaag ttgtttagta taaaaatact tgaacaggta tttatttgaa
     9841 atacaaacta aaaccctatt tttggagaaa ttttaaaact actccaaaat agggtttttt
     9901 atttctactt ttcaaatata ataaattcaa atataataaa attaaaaaca agtaaagtat
     9961 gttgttaaga aagtgctata aaattaaaaa tagtaatgta gaaaaaatga gcaataatct
    10021 tattttttct acaataattt tctataattt aaagtaagga aagtttgata aattataacc
    10081 ttagttaaag aataagagtt tattaaaaaa taaagtgtgt ataggggttt tagttaataa
    10141 aatattataa tgtaggggag cttgtaatga tatataaaaa agataaagta atgaaagttt
    10201 tagaatcatc atatagggaa aatgaaaaag ggattacagc taaccaatta tcagaaaaga
    10261 caggaattgc aagaaacaat ataagtacat atttaaacca attattcaat gagggtatgg
    10321 ttacgaagat aaaaggaaga cctgtgtatt atatccctgc aaatcttaac cttaacaaga
    10381 atgaattgct tgatacacaa agtgagttgc ttggcataaa aaaaggaaca aaagtagaaa
    10441 acaaaataaa aaaagatagt tttgaaacac taataggtaa agaacatagt ttaagaccta
    10501 taatagaaaa atgcaaggtc gcaatgctct atcctacaaa tggacttcat actattatct
    10561 atggagaaac aggagtagga aagagtatga ttgctagata tatgtacgat tattctatag
    10621 atagtggaat tagaaaaaat aaagctcctt ttgtaacatt taattgtgct gattatgcta
    10681 ataatgcaca attacttatg ggacatattt ttggagtaga acaaggagct tatacaggtg
    10741 cagaatcttc cagaaaaggt cttttggaaa ttgcaaatgg agggatacta tttttagatg
    10801 aaattcatag actaccagca gaaggtcaag aaatgttgtt tacttttata gataagggaa
    10861 catttaaaag actaggagat gcaacgaaag aaagagattc taaggtatta ataatatgtg
    10921 ctactacaga aaatccgaca tcaacactat tagatacatt caatagaaga ataccaatga
    10981 aaatagaaat accatcactg agagatagga caatgattga aagaatggaa ttagtcaaaa
    11041 attttttcaa ggaagaatca aaaagtattt ctatttcaat aaaagtacat aaagaaataa
    11101 ttaaatcact attactttat gattgtaaaa ataatgtagg tcaacttcaa aatgatataa
    11161 aattagctgt tgcaaatgga tacctaagat ataaaagaga taataaagaa ccagtttata
    11221 tagagaaaaa atattttgat aaatctataa atgaagtgag tgatgattat agagagaagt
    11281 ctattaaagc agatgaagtt ataacacatg gaatagaata ttttgtattt acgtcattag
    11341 gagaagaaga agttattaac tacaatgata taaatattat taatgcagta aatactaatc
    11401 ttaataaatc tgtagaaatg aatatagcag atgcttctct atttaataat gttaagttta
    11461 aaagcatgtg tgaatcaata agacaaatta ttaaagaaga tgaagaaata gatataagtg
    11521 aatcaatatt taagtcaatg gcaatttata taaaaagcgt attggataaa agtagctcta
    11581 caaacaacat agatttgaat caaattagaa gaaataacag aagtgaattt aaaacagcac
    11641 taaaaatagt tggaataatt gaaaatgaat ttgatttatt tttatctata gaaactgtag
    11701 catatattac attattcata gtacaagcta aacaagatga acaaaataaa atagtgaata
    11761 acattaatat tatagttgct atgcatggag aaactacagc aagtaatggt aaaagcagtt
    11821 ggtgatatta ttggaaagtg cagtgctatt tctttcgata tgaagttaga taaaagctat
    11881 gatgaagtta ttgtagattt taaaaattta attaataata tagacaataa agatgtatta
    11941 ctatttacag atatgggttc attaaactcc tttgatgaaa ttataaagaa agaaaaaaag
    12001 tgtgaagtaa gagtaattcc tatggtgacg acattaactg tgttagaagc agttcagaag
    12061 gccaatatgg gattaccttt aaatgacgta tacaactcta tcactaacac gagaaagtat
    12121 tattttggaa ctaatgaaat tcaaaataaa gaaaatctgt ctaaaacaat tattatagca
    12181 tcacatgttt cagaaggtgt agataataaa acaagaaaaa tattagaaga aaagatgagt
    12241 aggtacttag atggaataga tattatatct gtcccatata aaacagaaaa agatttaagt
    12301 ttaaatatta caaaacttaa agaaagtagt aatattgttg cagtaatcaa tgaacaaaga
    12361 attaatatta gaggaattga ctatattagc aaaaaagata tagataagga tgaaaatatc
    12421 aataaattaa aaaatattat aaagataagt ataggttatg atgatgtagt tgaaggttta
    12481 aagacatcct taaaaagctc taattataat aggatattta aagatataaa atatgtgtct
    12541 gatgaattgt ttttagtatt taatatagaa aagaaatacg ataaagtaat tggacttatg
    12601 atgcatttag ctttcatggt agatgggttg attggaaata ctagagaaat tgaaaagctt
    12661 gataaagaaa aaacacttga ttatcacaag agcctaagca aaataaaaga tatagtatct
    12721 caattagata aaaaatataa tatagaaatt aatgaaaaag agtgctatca aatattatta
    12781 atattagaat atgcagaaat aatcgaaaaa gattaccaat gagtataaaa acttatttgg
    12841 taattttttt tagtctaaaa taatattaat attagacaca tataaatata cttagacaaa
    12901 gtatctaagt tttgatgata aaaatgattg aaataacagt gttttaaagt gtctaaaaaa
    12961 actggcataa tacttgctta tattattaaa tgagtaatat aaataattaa aatttaaata
    13021 aaaaggagta aataatatgc aagataaaat attaagagat aaaaaggcaa atgacccatg
    13081 ggcaagaatg actacaagaa acggatttag tgcagacgaa ataatatcgt catttcaaaa
    13141 agctataaga agaaatatgg tggaagaagc ttgtgaattt gcttatgaac tatatatatc
    13201 atcaccagaa ttagaagata agctatggag aaggatactt acaatatcag ttgaagatat
    13261 aggaatgggg gacccatcag ctgtaataat cataaataat ttaaatcaag taagaaagga
    13321 atattcatat gcagatggag acagaccttt atttttcata catgcaataa gatatctttg
    13381 tgcatgtgag aaagatagat caagtgattt attaaaaaat ataattataa aaggatttgc
    13441 aatgggtaaa gttccagaag ttatggacgt ggcacttgac aagcatacat atagaggatt
    13501 agaaatggga agggattcat tccacttttt aaatgaagct agtatagtaa taccagaaaa
    13561 agaagtagat aatgattata aagaaagata cttaaaaata ttagaaaaat ataagcaaga
    13621 agaagttata gatacggctt tcaagtttaa tcattggcag tactaagaat tagagtaaat
    13681 attaataaga attaaaataa aataataaga attaaagtaa aaactgatag gaattagagt
    13741 aagaattggc tgataaaaaa ttagaaaata aaagaggtta gatattgtag gagggtaatt
    13801 atgaatattt tattagtatg tgcaaatgga gcttcaacag gtgtacttgt ggaaaagatg
    13861 aaaagttttt gtagtgagca tgaaaagtta aaaactaaaa ctataaatat agaggcaaca
    13921 tcattcgaaa atttaaaatc atacattgaa gctaatgata cagatgtagt acttgtagca
    13981 ccacaaatta ggttcaagga agatgatgta gttgaagctt gtaagaatta caaaatagca
    14041 gttggtttaa tagatactaa gcattatggt cgtatggatg ctccttctgt tatgaagtcg
    14101 gcaatcgaat tatataaaaa tagataattg gggggaataa aaatggaagc acttcaaaat
    14161 aatttaagaa gatttcttct accaatagca caaaagattg aaaaacaaag gcatcttcaa
    14221 gctataaaag aaggtatgat aagcattacc ccaataataa ttgttggttc tttaagtatg
    14281 ttatttatgg cattaaacaa tatgttacct gaaggaagtg caaaaacatt attatctgaa
    14341 aatatggata cattacttat ccctaataaa tttactatga gtttattatc tatatattca
    14401 gcatttttta tagctcaggc actagctaaa aaatataatc ttaatcatgt agaaataggt
    14461 atgacagcag ttgttgcaca acttgttgta tgtggacaag tagtagatgg tgtattagat
    14521 acaagttatt tagatgcaca aggtttattt gtaagtatat tagtagcact tttagtagtt
    14581 gatattacta agtttatgaa tgataaaaat ctcgtaatta gatttccaaa agaagtacct
    14641 agtgtagtaa ataaaagttt tagaaattta actccaatga tagtatgtat aatgttattt
    14701 acagcaatag cagcaataac taaaaatgtc tcaggtcaac cattaccagc tattataatg
    14761 aatttcttag caccagctat aagcagtgta gataatgttt ttgcagtaac tataatacta
    14821 tttataactc aattattatg gttctttggc cttcatggag cagcaataac ttcaagtata
    14881 tggatgccaa tagcagctac atatatggca gaaaatgcaa cactgatagc agcaggagga
    14941 gacccaaaat atgtgtttac aataggattt tactatggtt tcttgcaagt aactggttca
    15001 gggataactt tgggattagt gtaccttatg tcaagaagta agtgtaagag ttttaatagt
    15061 atgggaaaag ttgtgatttt accatcacta tttggaataa atgagccagt aatatttgga
    15121 acaccaatag ttatgaaccc atatatgttt gtaccatttg tatttggacc tgtattggtt
    15181 ggagcactta attttatggc attaaaagta ggattagtag gattacctat tgcagaacca
    15241 ccaggctttt tacctccagg ggttgcagca tttttgatga cattagattg gaaggcaatc
    15301 gtattagtat ttgcaagtat aatacttatg accttgatat attatccatt ctttaagata
    15361 atggagaagg aagaattaaa taaaaatgca gaattagcta caacattaga tgatgatagc
    15421 tttgattttt aacaagtcat tagaaggaga aggatatggt acctgaaatg gaagaaaaag
    15481 tatttagtat aatatcagaa gcaggagatg ctaaaagtga tgtaatgatg tctcttaagg
    15541 aaataaaaaa aggtgactac aataaagcaa aacacctttt aaattcagcc tcagagaaaa
    15601 tacaaacagc atcaaagcat catttagaat tactatcaaa tgctatgaat agtgaagata
    15661 gtgggactga ttttttagtt gtgcactctg aagaccatta ttcaaatgca ttatttgctc
    15721 attctttagt atctgaactt gtagatatat ttgagatgat ggacccaaga attaataaga
    15781 attaaatttc ttgtaaatag tgcttgaagt ttggctatag tttattaaat caatagattt
    15841 aataaatgaa ctccctttta ctttaaagta ctagtattta taaatttatt tataaataca
    15901 gtaaattttt aaatagaaaa ggagatatta ttatggaaaa attttcagta gtaatagctg
    15961 gaggaggaag tacatatacc ccagaaatca tattaatgtt gctagataac ttagataggt
    16021 taccattaag agcaatcaaa ttatatgata atgatgaaga aagacaaaat aaggtagcta
    16081 aagcttgtga aatactaata aaggaaaagg accctaatat agagtattta gcaacaacat
    16141 gccctaaaga ggcttataca gatgtagatt tttgtttagc tcatataaga gttggaaaac
    16201 ttgaaatgag agaactagat gaaaagatac ctctaaaaca tggagtagtt ggtcaagaaa
    16261 cttgtggacc aggtggaata gcttatggaa tgaggtctat agaaggtgtt cttgaaaaca
    16321 tagattatat ggaaaagtat tctccaaatt gttggatgtt aaattattct aatccagcat
    16381 caataatagc ggaagctgtt agaaggttga gacctaattc tagggtaata aatatttgtg
    16441 atatgccaat aggtatggaa cataatatag caaggattgc aggactcaaa tctcgtaaac
    16501 atatggatat aagatacttt ggattaaatc actttggttg gtatacttca attaaagata
    16561 aaaaaggaaa tgaactttta ccaaaattag tagaacatat aaaagaatat ggttttataa
    16621 atggtgaaga aggaatgaaa aatgacaaga aagatagttg gtttgaaact aatttattta
    16681 ctaaagaaat agtaaaaaca gatttaacta caataccaag tagttactta aagtactatc
    16741 tatttccaga ttatgttgta aatcattctg atgtaaatta tactcgtgcc aatgaagtta
    16801 ttgatggaag agaaaaagaa gtttttggtt catgtgcatt gatagaaaag caaggacatt
    16861 ctaagggcag tgggcttaag ataggaatac atgcagagtt tatagtggat ttagctacag
    16921 ctctagcttt taatacacag gagcgtatgt tattgatagt tcaaaataat ggagctatag
    16981 aaaatataca agatgatgct atggttgaaa ttccatgtat agttggaaaa gacgggtatg
    17041 aaccattatc aatagggaaa ataccaacat tccaaaaagg tttaatagaa caacaattag
    17101 cagttgaaaa attagtagtt gatgcttgga ttgagaagtc ttataataaa ttgtggcaag
    17161 cacttatgct atctaagaca gttcctagtg caacagttgc aaaagaatta ctagatgatt
    17221 taatagaagc aaataaaaat tattggccaa aacttaatta agaataatat atttttaaag
    17281 ttcctacagt ttagtacata gcctagttaa tgtatttgta gggactttat ttatttacaa
    17341 atgagataca agtggcaaac atagtgtatt ttgcagttag gaaagtaggt ttagttactt
    17401 gttagttgga ttaaatttag gtacaataat ttactatcaa tcaatagaaa taaccagaga
    17461 agttgcataa taaaaaattt acacttttaa caggatttat gtaggtgttt atagaaataa
    17521 ataaatatgt acataaatac ataattattt ttaagtctaa aatacaagaa tggagagatg
    17581 gctatgaagt ggagagatta tgctgatgat gtaaatttat ttgaggattt tgataaaact
    17641 atcaagcctc taactgacga acaaagaaga aaaaatataa acactttaat tgcatatttt
    17701 tcaaaagaag ttccttctaa aatatacaat ttatcaaatg atgaaattaa gcctagagat
    17761 atacttagag gacttttgaa tgtatatcca ccaaaagaaa ttgctccaga gatacttaat
    17821 atgttacata atttattgtt gattgaatgt gaagaaagag agttagtaga tgtaaatgat
    17881 atagaagaag tagaggaagg gattgccata tggagaggta atataacgaa tttaagagca
    17941 gatgcaatag taaatgctgc aaataacaag ttattagggt gtttacaacc attacatcta
    18001 tgcgtggata atgagataca ttcatgcgca ggtcctagac ttagagaaga ttgtgacaaa
    18061 ataataaaaa aacaagggca tttagaatat actggagatg ctaagataac tagaggctat
    18121 tgtttacctg caaaatttgt agtacatact gttggaccta ttgtatctgg tggtcagcca
    18181 agtaaagagc aagaaaagca actgcttcat tgttataaat catgtttaaa tacaataaaa
    18241 gaaattgatg aaataaaaaa tattgttttt tgtggaattt ctacaggtgt atttggttat
    18301 ccaaaaaaag aggcagctaa tcttgctgta agtagagtta gactatggct aaaagaaaat
    18361 cctgaaaaaa atttaaaagt tgtatttaat gtgtttactg aagaagaaga agagaaatat
    18421 agaagaatat ttaaataata ttatgtaaca gaatcctaaa aatacttatt atataataca
    18481 aattcattgt ataagataat aatttacaag tagtaaaaat taataaaata taatt
//
LOCUS       DAELXX010000081        18475 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.081, whole genome shotgun sequence.
ACCESSION   DAELXX010000081 DAELXX010000000
VERSION     DAELXX010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18475
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.081"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            178..546
                     /locus_tag="KPH29_002614"
     CDS             178..546
                     /locus_tag="KPH29_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG1468200.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            549..920
                     /locus_tag="KPH29_002615"
     CDS             549..920
                     /locus_tag="KPH29_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HBG1468201.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(1064..1519)
                     /locus_tag="KPH29_002616"
     CDS             complement(1064..1519)
                     /locus_tag="KPH29_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468202.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(1558..2823)
                     /locus_tag="KPH29_002617"
     CDS             complement(1558..2823)
                     /locus_tag="KPH29_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HBG1468203.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
     gene            3293..4744
                     /locus_tag="KPH29_002618"
     CDS             3293..4744
                     /locus_tag="KPH29_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBG1468204.1"
                     /translation="MNFLNGILTSINNAVVFVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLEKVSEWHRELSEEHLG"
     gene            complement(4964..5425)
                     /locus_tag="KPH29_002619"
     CDS             complement(4964..5425)
                     /locus_tag="KPH29_002619"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBG1468205.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGRYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLN"
     gene            complement(5531..6724)
                     /locus_tag="KPH29_002620"
     CDS             complement(5531..6724)
                     /locus_tag="KPH29_002620"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HBG1468206.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKMGH
                     LLDII"
     gene            complement(6747..7412)
                     /locus_tag="KPH29_002621"
     CDS             complement(6747..7412)
                     /locus_tag="KPH29_002621"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBG1468207.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSSNHKSNIDKDFLFK
                     NGF"
     gene            complement(7446..8549)
                     /gene="ribD"
                     /locus_tag="KPH29_002622"
     CDS             complement(7446..8549)
                     /gene="ribD"
                     /locus_tag="KPH29_002622"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBG1468208.1"
                     /translation="MNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKDSNIIGK
                     GYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKRVVI
                     STLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKSAVS
                     LDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQEKVS
                     HPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSKNNL
                     VDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPTFVS
                     GEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(8681..8802)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            8991..11474
                     /gene="recQ"
                     /locus_tag="KPH29_002623"
     CDS             8991..11474
                     /gene="recQ"
                     /locus_tag="KPH29_002623"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBG1468209.1"
                     /translation="MNKKLLEILSKYYGYTSFRKGQESIINSILSEKDVLAIMPTGGG
                     KSICYQIPALILDGMTIVISPLISLMKDQVDALKTMGISAAFINSSLSSKEFNEILNN
                     IRKNNYKILYIAPERLDAQEFIELINNNNVSQVAIDEAHCVSQWGHDFRLSYRRISDF
                     IKNLPKRPIVTAFTATASEEVRTDIINLLCLENPDYYITGFDRENLSINIVKSSSKNK
                     YILDYIQNHKNESGIIYVATRKEVENIYNGLSKRNISVSKYHAGLSKNERSKNQEDFI
                     NDNVDIMVATNAFGMGIDKPNIRWVIHYNMPQSIENYYQEIGRAGRDGEKSECVLLFS
                     PGDVHIQKYLIDIGVENPERKLFQHKKLQYMIDLVYSNSCYRKTILSYFGENYIDNCN
                     NCSNCLVEGEIVDKTIDAQKVISCVARMKRSYGVTTIVDVLRGSKNKKILQLGLNTLS
                     TYNIMRDYSSEDLKNFVNTLVSHGFLDLVETLGNGNRGSFPTIRLNEMSMKVLKGEVK
                     VEFKEIVMTKSLEVEDELYNALRELRHSIASEERIAPYMVFGDGTLRAMSSSYPINKE
                     EMLDISGVGEIKYQKYGRDFEAVIKEYVEKNNINKKIDEKNVDLKNVNSDFLEVNTDK
                     VLYEKLLVIREEYATKEKLPAYMILANKALKEISGRYPLDEEQLKDISGIGGVKFEKY
                     GANILAEVNKYVKENNINVIWEKKGKRKLILDGESRKNNEIALDLLNQGKDIQSISQE
                     IEVSLSTIIGYVHDYVKDGHSISFELGLENFYNEDKKKMILEVCEKVGYDNLNNIKRK
                     LPDSIKYENIRAVMLDSYLENLKNNIIAE"
     regulatory      11562..11673
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11815..13116
                     /gene="thiC"
                     /locus_tag="KPH29_002624"
     CDS             11815..13116
                     /gene="thiC"
                     /locus_tag="KPH29_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBG1468210.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            13170..13364
                     /gene="thiS"
                     /locus_tag="KPH29_002625"
     CDS             13170..13364
                     /gene="thiS"
                     /locus_tag="KPH29_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBG1468211.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            13357..14160
                     /gene="thiF"
                     /locus_tag="KPH29_002626"
     CDS             13357..14160
                     /gene="thiF"
                     /locus_tag="KPH29_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HBG1468212.1"
                     /translation="MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMPLCENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            14223..14993
                     /locus_tag="KPH29_002627"
     CDS             14223..14993
                     /locus_tag="KPH29_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBG1468213.1"
                     /translation="MDKLVLGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            15010..16113
                     /gene="thiH"
                     /locus_tag="KPH29_002628"
     CDS             15010..16113
                     /gene="thiH"
                     /locus_tag="KPH29_002628"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBG1468214.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEAISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYEFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            16114..16803
                     /locus_tag="KPH29_002629"
     CDS             16114..16803
                     /locus_tag="KPH29_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBG1468215.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKECRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIK
                     KTISNYNEKLN"
     gene            16991..18385
                     /gene="yfcC"
                     /locus_tag="KPH29_002630"
     CDS             16991..18385
                     /gene="yfcC"
                     /locus_tag="KPH29_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBG1468216.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
BASE COUNT         6867 a         2250 c         3033 g         6325 t
ORIGIN      
        1 taataaaagt attttaaaat ctatatgtaa ttacttttat taataaaatt caaagttata
       61 ttagtagaaa ttggaattat ttataatata ctccaaaatt taaaaataat ggttgaaaaa
      121 tctaattaag agtactataa tttaattaag taattactta atcaaaacga ggtacttatg
      181 gaaaacttgg ttaaaatatt taaagcattg agtgatgaaa ctagacttaa catacttata
      241 ttagtatcca agagaaatat atgtcaaaag gggatttcta aatatctagg gatttcagat
      301 tctgctgttt cacaacatat aaaaatatta aaagatgttg ggataataac aggatataaa
      361 gaaggatact atgttttata tcatattaat aaagaatcat ttaatatatg tgtagatttt
      421 ataaattcta tgttaagtaa ttctagtgaa agctttatag atgtatttga tgtaaataca
      481 atacatctag gatgcagtaa agaatgtaaa tctataaaaa aatgttgtaa gagaagggag
      541 aaataataat gaaagtatgt ataccagtag aggagaataa aggattggat agtaaaccat
      601 atggacattt tggttctgct ccaatttttg ttgtgtgtga tttagaaagt ggagaagtta
      661 aatctttaga taatggagat ttagatcatg aacatggaaa atgtcaacca ttaaaagcat
      721 tgtcagggac agctgtagat gcagtagttg ttggaggaat aggacaagga gctataatta
      781 aattaaatgg aatgggaata aaagtttaca gagcagaagg tgatactata agtgctaatt
      841 tagacttact taaaaatgga aaattagtag agtttccatc agaccatact tgctcacatg
      901 acggatgtgg acatcattaa aaaaaacagc tgatgaaata tatacttcat cagctatttt
      961 ttcttttaat aaatacgatt ttattctttt aaaaccaaaa tttagatttt tatgtttttt
     1021 gtagtatcct taataatatt tcaagtttac aagtttacta cttttagatt ttttttgtat
     1081 gtttttctat atagtcaata tcatttaata tgtcattgag ttctttatta agtgttttta
     1141 aatcatcacc atctacattg tagtttaaaa atagttcctc tacattatca atgatagaat
     1201 tgacttttct agtaacttca acaaatgtct taaagttact actattttta gcatttagaa
     1261 atttttttaa atcctttgat ataaatgata ctatttcata ttcatcatta aatagacctc
     1321 taccaatagg atatggttca attctctgta aaaaatattg gtctttagca ttaatattat
     1381 aagtataaga tatttcccaa ccgtctccta aatcaaactt gataaaatat aatgtactac
     1441 ttacttttga tattcttgtg gcaccaagct gtcttaaaag aacttcaatc gaatcacctt
     1501 gaacagtatt aatattcata tatacacatc ccctttagta ttattatact atatttttta
     1561 aaattttata attaaataaa cagtagatat aataatagat ataatcataa gtggaaaacc
     1621 tattttgaaa tactccttaa aagagatagg atgaccgtgt ttattaccta tacctgctag
     1681 tacaacatta gcagatgctc ctattagtgt tccatttcct ccaagacaag ctccaagaga
     1741 tgttgcccac catagtggca ttacatcaat accttcagct tgcatagtca aaataagagg
     1801 aattagtgtt gcaacaaatg gaatattatc taagaaagaa gagactatag ctgataacca
     1861 taatatcaac aacattgtaa atacaagatg tccttctgta agtccaatca atgcttgagc
     1921 caatttgttt attatcccaa cttctactag tccaccaact actacaaata atcccataaa
     1981 gaataaaatt gtggaccatt caatacctgc cataatttca tctacatctt gttttcctat
     2041 taaaagcatt atagatgctc ctgtcagtgc tacaacagaa gaatctatgt ggattgtact
     2101 gtgaaacata aaaccaaaaa gtatgaatgc tattaatata aggcttttta taagtagagg
     2161 tttatcttta acagattttt tttcatctag ttttaatatt gcatttttag ctctttcatt
     2221 cacaacaagt tcttttccat aaataaatct aaagcatatt atagttatag caagtattac
     2281 tataacagca ggacctaaat ttattacaaa atccataaaa ctaagattgg cagcactacc
     2341 aatcataata tttggagggt ctcctataag agtagctgtt cctcctatat ttgaagctaa
     2401 aatctgtgtt attaaaaatg gaactggatt aagtcctaat atttgtgtaa taactatagt
     2461 cattggtcca ataagtaaga cggttgtaac attatctaat acagctgata aaatagctgt
     2521 tataatagca aaacaaatca ttatcttcca tgggtcacct ttagctttct ttgcagtcca
     2581 gatagctata tattcaaata accctgagtt tttgacaaca gctacaaata gcatcatacc
     2641 aactaataca ccaatagtat tgaaatctat atggcttaat ccttcatcaa gtgtaagtat
     2701 attgaatatt atcattaata gtgcaccacc aacagctgct acggatctgt ttaccttttc
     2761 tgtaataata gctgccataa ctattaaaaa tatacatata gctattattt gttgaaattc
     2821 cataatataa gtacctccat ttgtattatt ttgtgcctaa tgctagtact atatatagta
     2881 ttactttttt attgtttttt caatggatgt atgaaagtat attatttaac aaagtttttg
     2941 agaaattgac agaatttaaa gaaaattttt gttatttaaa taaattttaa aattattcca
     3001 ttgataataa tattaaacga caaacgaaaa aatataccct ttaaactaga atttattgta
     3061 tccaataaca attataaaat attaaatata tctatattat aaaaataata aaaaaaggtt
     3121 tgatttgaat ataaaactgc aatatattaa aaaaattatt cattttaaac cttgtaattt
     3181 gattttataa atattataat taaattaatg aatttttaac aaggaattat tcattttttt
     3241 gattttaaga tttattgaga aaacaaaact aaataggggg gtaattaata taatgaattt
     3301 tttaaatggt attttaacga gtattaacaa tgcagtagta tttgtaaaca attacttatg
     3361 gtcgtatatc ttaattataa tgttagttgt aattggtatt tactttacta ttaaaacaaa
     3421 ttttgtacaa ttcagatatt ttatagaaat gtttagactt ctgggtgatg gtacagcgaa
     3481 taaaaatgca aaaaaggaag gaaaaatctc ttcatttcaa gcattttgta taagtacatc
     3541 ttcaagagtt ggaactggaa atatagctgg aatagcaatt gcagtggtag cagggggacc
     3601 tggtgctgta ttttggatgt ggcttatagc attaattggt tctgcatcaa gctttgttga
     3661 gagtacatta gctcagattt acaaagtgaa agatggtcaa gcttttagag gtggaccagc
     3721 gtattatatg gaacaaggtc taaataaaaa atggatgggt attttattct caatacttat
     3781 aactatatgt tatggttttg tatttaatgc agttcaagca aatactgttt cacttgcatt
     3841 taataatgca tttggaataa gtaaaatggc tatgggaata ggtcttggaa ttttaactgc
     3901 aatagttatt tttggtggag tgcatagagt tgcaaaagtt tctgaaataa tagttcctgt
     3961 atttgcagga ttatatatat tagttgcttt ggctatagtt gtaatgaata taacagagat
     4021 accaagtgtt atagcattga tagttgaaag tgcatttgat tttaaaggaa tggcgattgg
     4081 tacatttatg ggagttgtaa tgactggagt gaagagaggt ctattttcta atgaagcagg
     4141 tatgggtagt gctcctaatg ctgctgcaac agcacatgta actcatcctg taaaacaagg
     4201 tttaattcaa tctttaggtg tttttacaga tactataata atatgtagtt gtacagcatt
     4261 catggtatta ttatattcag gttatagtag tagtggagca actggaattg aattagctca
     4321 agaagcttta actcatcata taggaccagt tggaaatata tttatagcag tttgtatatt
     4381 cttgtttgca tttagttcta tagttggtaa ttactattat ggtgaatcaa atatggagtt
     4441 tatgagtgga agtaagacta aacttaatgt atttagagtt tttgttgtag gcatggtttt
     4501 atttggttca cttactcaag tagatgtagt ttggaatctt gcagacttat ttatggcatt
     4561 gatggcgata ataaacttaa tagcaatagc acttttagga aaatatgcat ttattgcttt
     4621 aaaagattat acaagtcaaa aaaaatcagg aataaaagac cctgtgttta ttgcagatga
     4681 aatagaaggc ctagaaaagg tatcagagtg gcatagggaa ttatctgagg aacatttagg
     4741 ataaaaaata gtataaaatt ataaaaccat tttgaaagac tagagtatgt atatgcttta
     4801 tatgaatata atattgaatt aatttatatt actggaattt ttaattccag taatattttt
     4861 ttattatata atttagcttt attaaaagtt tttattatta aaaatagctt atttatcgta
     4921 aagtgaatga tattggtttt ttaattatta tgaatgttgg tatttaattt aaagacttaa
     4981 ataaatttgc catttctagc gcattcatag cacaatcata acctttattt ccagcttttg
     5041 tacctgctct ttcaatagct tgttcaatat tttctgtagt tacgattcca aatataacag
     5101 gtaattcact gtctaaagat acttttgcta tccctttaga gacttcacta catacataat
     5161 cataatgaga agtagcccct cttataacac agcctaaaca gattatagca tcatacctac
     5221 cacttttagc cattttttga gcaacaagag gaatctcaaa agctccaggt acccaaacta
     5281 tttctatatt ttctggagaa acgtcatgtc ttaaaagaca gtcttcagct cctgataaaa
     5341 gttttgatgt tataaattca ttaaaacgag aatttattat acctattttt aaatcttttc
     5401 caataagttt tccttcatat atcattttta tttcctccaa ttttatttat gttatagatt
     5461 tatatttata aatatcttta attttaatta taaaaaacta cttagtagaa aaatatcatc
     5521 atataatcat ttatataata tctaaaagat gtcccatttt atctttttta gtttttaaat
     5581 atagcttatt aaagtcatta atttcttcat taatagggat tctattttcg atttttattc
     5641 catatttctc taattgattt attttatccg ggttattact taaaagattt atacttttta
     5701 tgtttaaatt ttttaatatt tgagcagcca tatagaaatc tctcatatct tcttcaaatc
     5761 caagcattaa atttgcttca acagtatcgt aaccttcttc ttgaagttta taagccttaa
     5821 ttttatttaa aaggccaata cctctacctt cttgcttcat ataaattaaa actccacttc
     5881 cattttccac aattttcttc atagaagatt ctagctgtaa tccacaatca cattttagag
     5941 agtggaacac atctcctgtt aaacattcag aatgaagtct aactagagta ttttctatat
     6001 ttatatttcc ataagtaagg gcaatgtgtt cttcatttga ataagtatct ctatatccta
     6061 tgatttcaaa atttccatat tttgttggaa ggaaagcact agatactttt tctatagtag
     6121 tttcatttat tttgcggtat tctataaggt cttttatagt gatgattttt aaattatgct
     6181 tttctttgaa aatcattaaa tcatcaactc ttgccattgt accatcatct ttcattattt
     6241 cacagataag accaatctct ttaaaacctg caagtctagc aaggtctact gaggcttctg
     6301 tatgtccatc cctgacaaga actccaccag tttttgctat taaaggaaat atgtgaccag
     6361 gcgttttgaa atctttactt gtagaatttt catcacagaa caattttaca gtttcagctc
     6421 tttcatatgc agatatacca gttagagtat ctgctccatc tatagtaaca gtaaaggctg
     6481 tttgaaatgt atctgtattt tctctaacca taagaggtat attaagactt ttaaattttt
     6541 tttcagtggc aggtagacaa attagacctc taccatatgt agccataaaa tttattgatt
     6601 caggagtagc catttcagct gccatcaata aatctccttc attctctcta ctttcatcat
     6661 caactacgat taccattttc ccatttttta tatcttcaat agcttcttca atagtattaa
     6721 acatgtaatt cactccttta ttatttttaa aaaccatttt taaataaaaa atctttatct
     6781 atatttgatt tatggttact actattagaa tttaattcct tgtagttatt agccataaaa
     6841 ttattaacat actttccaat tacatcgctt tctaaattaa caaaatctcc aacattcttt
     6901 gtcaagagaa tagtgttaat tttagtatga ggtataatag aaaccttaaa tatttcatca
     6961 tctaaatatg atatagttaa acttacacca tcaatagcaa cagaaccttt gagtatcatt
     7021 gaagataaaa gttttttatc aggtcgtata cttactaaaa ctgcattttc atctttttca
     7081 aatttgcaga tagttccttt accatcaaca tgacctgtta ctatatgacc accaaatctt
     7141 gaagaaagac tcatagctct ttcaagattt accttatcat ttgcatttaa taaacctaaa
     7201 tttgtggaac gaattgtctc catcataaca tcggctgtaa attcattttt tccgaggttt
     7261 gaaacagtta gacaaactcc atttacggct atactatctc caagatttgt gccatctaat
     7321 attttatttg atagtatggt tacttttcca ctcttaccat tagcagtaat ttttctaagt
     7381 attccaactt cttctactat gccagtaaac atcctaacct cctatatgaa tgtattaaca
     7441 aaaatttaat ttaaaacatc tgattctata agaatatcgc catctatcaa agttacatta
     7501 ttgattgtta actgtgttga atctgacaaa tgatttattc cttctccaga aacgaatgta
     7561 ggtgcatctt taccacctat aatcttgggt gatagaaata atttgacttt atcaactaac
     7621 ttatttctaa agagactgtc attgagagta gcaccacctt caactagaac agaatcaatg
     7681 tttaactctc ctaatttata cattacaaat tccaaatcta ctaagttgtt ttttgaagga
     7741 gaaattattg tttcaacatt attttctttt aacatagata attttatact ttctttgcaa
     7801 catgtgaaaa ctatagtttt actggtttta tctttaacca aattactatt aagtggcaac
     7861 tttaggtgtg tatctattac tattcttgta ggatgagaca ctttttcttg attgagcctg
     7921 caagttagta aaggattatc gttcaaaact gtatttatgc caaccattat actttggtat
     7981 ttatttctgt atttatgagt tagatacctt gatgattcat tagatatcca ttttgattct
     8041 agtgattttg tagcaatttt accatctaga cttactgctg attttacaat acaaagaggt
     8101 cttttgtttt ttatatgata aaaaaatgcc tcatttaaat ctcttgcttc atcttctaat
     8161 attcccacag taacatcaat attgttatct tttaattttt ttactccatt accacaaaca
     8221 agtggatttg ggtctaatgt agatattaca actctcttaa tcttattttg tataatttta
     8281 tctacacaag gaggcgtttt cccataatga gaacatggtt ctaagtttac ataaagagta
     8341 gagtctttta gactttgttt agcactgttt atagcattta cttcagcatg attagagcca
     8401 aatttctcat gatatccttt gcctattata ttactatctt taacaattac acacccaact
     8461 aatggatttg gatttacaaa gccttctcca tttttagcca attcaatagc ttttttcata
     8521 taataaatat ctttttcttt ttgattcaca atatgtacac ctcctgttta attggataat
     8581 ttttaaacaa aaaaccctga gaattaactc agggtttaga ataatctaca ataaatgttt
     8641 aataaaagta gaagaaaatt gctctacaaa aacactcatt aaatatcttc ttccatccag
     8701 actttactgt cggctttgga atttaaccaa atcaaccaag ttttaccttg gctcgcgggc
     8761 tataccgccg attgggaatt tcaccctacc ccgaagatta agtattaaat ttatatttag
     8821 aatgtatcat aaattatttt actttgcaaa ctattttttt aaaaaatata tgttaatata
     8881 taatatagaa tgtctataaa ctttgaaata ccacgattgt gaatataaaa ataaaaattt
     8941 gaacataatc aggaaagaag tctaattaat ttaaattagg agagtaatat atgaataaaa
     9001 aacttttaga aatactatca aaatactatg gatatacaag ttttagaaaa ggtcaggaat
     9061 ctataataaa ttctatctta agcgaaaaag atgtgttagc tattatgcca acaggagggg
     9121 ggaaatcaat atgttatcaa attccagctt tgattttaga tggaatgaca atagtaattt
     9181 ctccacttat atcacttatg aaagaccaag tagatgcctt aaagactatg ggaattagtg
     9241 ctgcttttat aaatagttca ttaagcagca aagaatttaa tgaaatatta aataatatta
     9301 gaaaaaataa ttataaaata ctctatattg ctccagagag attagatgca caagagttta
     9361 ttgagctaat aaataataac aatgtaagtc aagttgcgat agatgaagca cattgtgtat
     9421 ctcaatgggg gcatgatttt aggttgagtt atagaaggat ttctgatttt ataaaaaatt
     9481 tgcctaaaag acctatagtt acagcattta ctgcaacagc ttcagaagag gtaagaactg
     9541 atataataaa tttactgtgt ttagaaaatc cagattatta tataactgga tttgatagag
     9601 agaatttatc aataaatata gtaaaatcat ctagtaaaaa taagtatatt ttagattata
     9661 ttcaaaatca taaaaatgag tctggaatta tatatgttgc aactagaaag gaagttgaaa
     9721 atatatataa tggtttatct aagagaaata tatctgtatc aaaatatcat gcaggtttat
     9781 ctaaaaatga aagaagtaaa aatcaagaag attttataaa tgataatgtg gatataatgg
     9841 tagcaacaaa tgcatttgga atgggtattg ataaacctaa tataagatgg gttatacatt
     9901 acaatatgcc tcaaagtata gaaaactact atcaagaaat aggtagagct ggtagggatg
     9961 gagaaaaaag tgagtgtgta cttttatttt ctccaggaga tgtacatata caaaaatatt
    10021 taattgatat tggagttgaa aatcctgaaa gaaaattatt tcagcataag aagcttcaat
    10081 acatgattga cttagtttac agtaattcat gctatagaaa gacaatattg agttattttg
    10141 gagaaaatta tatagacaat tgcaataatt gtagcaattg tttagtagag ggtgaaatag
    10201 tagataaaac tatagatgca caaaaagtca tttcatgtgt agctagaatg aaaagaagtt
    10261 atggtgttac tactattgta gatgtactta gaggttctaa gaataaaaag attttacagc
    10321 ttggattaaa tactttgtct acatataaca ttatgagaga ttattctagt gaagacttaa
    10381 agaattttgt aaatacattg gtttctcatg gttttttaga tttagttgag actctaggca
    10441 atggaaatag aggtagtttt cctactataa gattaaatga gatgtctatg aaagttttaa
    10501 aaggtgaagt gaaagtagag tttaaagaaa ttgttatgac aaaatcattg gaagtagagg
    10561 atgagttata taatgcttta agagaactta gacattcaat tgcaagtgaa gaaaggatag
    10621 ctccatatat ggtatttgga gatggtacac ttagagccat gtctagtagt tatccaatta
    10681 ataaagaaga aatgctagat atctctggag taggagaaat taaatatcaa aaatatggta
    10741 gagattttga agcagtaatt aaagaatatg tcgaaaaaaa taatataaat aaaaaaatag
    10801 atgaaaaaaa tgtcgattta aagaacgtta atagtgattt cttagaggtt aatacagata
    10861 aagttttata tgaaaaactg ttagtcatta gagaagaata tgcaacaaag gaaaaattac
    10921 ctgcatatat gattttagcc aataaggctt tgaaagaaat aagtggaaga tatccacttg
    10981 atgaagaaca attaaaagat atttctggca taggtggagt aaaatttgaa aaatatggag
    11041 ctaatatttt ggcagaggtt aataaatatg taaaagaaaa taatattaat gttatttggg
    11101 agaaaaaggg caaaaggaag ttaatattag atggagaaag tagaaaaaat aatgaaatag
    11161 cattagattt gctaaatcaa ggaaaagata tacaaagtat cagtcaagaa atagaagttt
    11221 cattgtcaac cattattgga tatgtgcatg actatgtaaa ggatggacat agtatatcat
    11281 ttgaattagg actagaaaat ttttataatg aagataaaaa gaagatgatt ttagaagttt
    11341 gcgaaaaagt tggctatgat aatttaaata acataaaaag aaaacttcca gattcaataa
    11401 agtatgaaaa tataagagct gtgatgcttg actcttatct tgaaaactta aaaaataata
    11461 ttatagcaga gtaattttta acttaataaa aataaaattt gtattattgt gttgaactaa
    11521 taattttgct gtgttatact gaatttgtaa aaataatgaa aaattagagc taggggcgct
    11581 agaattggct agctgagaga taaagccgta acttacaaac cctaataacc tgatcaagat
    11641 aatgcttgcg aagggaagct ttttaatata attttattat ataaataggc aaattattca
    11701 atgttaggag aaatttgtct aagtgtatat acgattataa gagtgctttc caaacgagga
    11761 aaagcacttt ttttattttc aaaaactata actttaatct tggaggattg taatatgaac
    11821 tatacaacac aaatggatgc tgctagaaaa ggcataataa ctaaagaaat ggaaatcgtt
    11881 tctcaaaaag aacaggtaga tgtaaatgaa ttaagagaat taatagcaaa tggacaagtt
    11941 gttataccag ctaataaaaa tcataaatct ttaagtgcag aaggtgtagg taaaaattta
    12001 agaacaaaaa taaatgtaaa tctaggaatt tcaagagatt gtaaggatat agaaaaagaa
    12061 cttgaaaaag ttagagtagc aatagacatg aaagctgaag ctataatgga tttaagtaat
    12121 tatggcaaaa ctagagaatt tagagaaaag gttgttgaaa tgtcacctgc aatgataggt
    12181 tcagtaccta tgtatgatgc agtaggatat ttggaaaaag aattaaaaga tataacagaa
    12241 gaagagtttt taaatgtaat aagacaacat gctattgatg gagtagactt tattactata
    12301 catgcaggtc ttacaagaag tgtttgccaa aaaataaaaa atcatgagag attgactcat
    12361 atagtatctc gtggaggttc tttattgttt gcatggatgg aattaaataa taaggaaaat
    12421 cctatttata ctaattttga taaaatattg gatatatgtg aagaatatga tgttacattg
    12481 agtttaggag atgcttgtag accaggttgt ataaaagatt ctactgatgg tgttcaaatt
    12541 caagaacttg tagtattagg tgaacttact aaaagagcat gggaaagaaa tgtacaagta
    12601 atgatagaag gtcctggaca catggctatt gatgaaatag aagcaaatgt agtattagaa
    12661 aagagattat gccatggagc acctttttat gttcttggac cattggttac agatatagca
    12721 ccaggttatg accatataac aagtgcaata ggaggagctt tagcatgtgc taaaggagta
    12781 gattttttat gttatgtaac tccagcagaa catttaagat tgcctaattt agatgatatg
    12841 aaagagggta taatagctgc aaagatagct gctcatgcag gagatatagc taaaaatgtt
    12901 aaaggtgctc gtgaatggga taataagatg agtaaagcta gagctgattt agactggtgt
    12961 gagatgttta gacttgcaat agaccctgaa aaagctaaga gatacagaga tgaatcaact
    13021 cctactcatg aggatagttg tactatgtgt ggaaaaatgt gctcaatgag aacagttaag
    13081 aaaatattaa acaatgagga acttaatcta atataatttt tggctagtat gactggaata
    13141 aaaagaagct gaaaagagga ggtgatttag tgaaggttaa tgggaaggaa attgaatttg
    13201 aaaaagattt gactgttata gatttattaa ataaatataa tttaaaaagt gatagagttg
    13261 tagttgaagt gaatttagag ataattgaag aatctaacta caatacatat gtattaaaag
    13321 acgaggacat tgtagagctt attagcttta ttggaggtgg ttaaaataaa aatatttgta
    13381 aatgaattat taacagatgt tgaggaaaat acaactgctt ataaagttag agatagctat
    13441 aacaataaat gtgatgtatt ggtgttaaat ggatatccaa ttaaaacaga tatgcccctt
    13501 tgtgaaaatg ataaattaac acttatacaa aaaggagtta agccatcact tgatgaattg
    13561 gaaagcttgt tgattgctag acatactcca aatatacata ataaattaaa aaaagggaaa
    13621 gttgctatat tgggattggg gggacttggt tccaatatag ctatctcttt agctagaata
    13681 ggagtgggag aacttttact tatagattat gatgttgtag aaccatctaa tttaaataga
    13741 caacagtatt ttatagatga tataggaaaa ctaaaaactg atgccatgat agaaaatata
    13801 aaaaaaataa atccatttat aaaattaaat aaacgagata tttttttaaa caagaacaat
    13861 atggatacta ttaaggattc agatttaata attgaggcct ttgatgatgc ttcatgtaaa
    13921 gcacaggtat gtaattatgt attaataaat ttaaaagata aatatttaat agcatcatct
    13981 ggtatggcag gttactatga ttcaaatata ataactacta agaagataaa agacaggttt
    14041 tatatatgtg gggattttgt gaatgaagct aaatttggtg aagggcttat ggctccaagg
    14101 gttgccatct gcgcaaatca tatggcaaat ttggcaacgc aaatacttat agatatgtaa
    14161 atattaaaat tagatataaa aaagattgaa tattaaaaaa atttaacgtg gagggaaata
    14221 atatggataa attggtactt ggtggacatg aatttaatag cagattattg gttggaacag
    14281 gtaaatatgg ttcaaataat atattgcctg aggtaataaa agaaagtgga agtgaaataa
    14341 taacaatggc acttagaaga gtggatttag acaataaaca agaaaacata ttaacgtata
    14401 ttccaaaaga gatgacaata cttccaaata catcaggagc tactaatgca gaagaagctg
    14461 taagaatagc tagaatatct agaaaaatgg gatgtggaga ttttataaaa atagaagtaa
    14521 tatcagatac tagatattta cttcctgata atgaagaaac tataaaagct acaaagatac
    14581 ttgctgatga ggggtttata gttcttccat atatgacacc agacctttat gctggaagaa
    14641 gacttataga agctaatgca gccgcagtta tgccacttgg agctccaatt ggttcaaata
    14701 gaggtcttca aatgaaagaa atgataagaa taatgataga tgagttggat ataccaataa
    14761 tagtagatgc aggtattgga aaaccatctc aagctatgga ggctatggaa atgggagctg
    14821 atgcagtact tgtcaacact gctatagctt cagcaggaga ccctgtacaa atggctagag
    14881 catttaaatt agctgtagaa ggtggaagag aagcatatat tgcaaaaaca ggaaatgtgt
    14941 cagaatttgc aaatgcatct tctcctttaa caggtttttt aggcaattta tagaaagtgg
    15001 tgattggtga tgagttttta tgatgtaata gaaaaatata gggattttga ttttgatgga
    15061 tatttaaata atgttactga taatgatgtt ttaagaagtt tatcaaaaga caagcttgaa
    15121 gattttgata tattaaattt gctttctaaa actgcagtaa aacatttaga agatatggca
    15181 caaaaagcac ataaattaag tgttcaatac tttggtaaaa cagtatgttt atatacacca
    15241 atgtatatag caaattattg tgttaatcaa tgtgtttatt gtagctataa tataaagagt
    15301 ggaataaaaa gaaaaaaatt aactatggat gaaattagag aagagggaga agcaatttca
    15361 aaagaaggtt tcaaacatct acttgttttg actggagaaa gtagttttca ctcaagtgta
    15421 gaatatatag gtgaggctat agaaatatta agagagaaat ttccctctat aggtatagaa
    15481 gtttatccaa tggaagtaga agaatataag tacattgtag ataaaggtgt tgaaggatta
    15541 acagtatatc aagaaactta tgacgaggaa atatataaga gagtacatat aaaaggccca
    15601 aaatcaaatt atgaatttag actagatgct cctgaaagag gggcaaaagc aggtatgagg
    15661 actttatcaa taggtgcact tttaggtcta aatgatttta gaaaagaaac tttttttaca
    15721 atattgcatg ggaaatactt aaaaacgaaa tatcctcata ttgagctttc atattcaaca
    15781 ccaagaatga gaccttttaa agggtgtttt gaggaattgg tagatataag tgatactgat
    15841 ttagttcaag ctatggtatg tatgagatta tttgaccctc atgcagctat aaatatatcc
    15901 actagagaaa atttagaaat gagaagtcat ataatacctt taggagtaac taaattgagc
    15961 gcaggtgtat ctactgatgt aggaggtcat tctcaagatg aacatgatac tgctcaattt
    16021 aagataaatg atgaaagtac tgttaaagat gtggagaaaa tgctaaactc aattggttat
    16081 cagcatgtgt ttaaagattg ggaaagattt taaatgtatc tgataacaaa cagaaaatta
    16141 tgtagtgagg aacgatattt agaagttatt aaagagtcta tattgagtgg tgtagaaaat
    16201 ataataataa gagaaaagga tttggaatat caagagctaa gaaaattata tatgaaaata
    16261 aaaactaaaa tcaattgtat agattttcaa gagcaaatat cagatgaaag tttgaaaact
    16321 aatataaatc aaaaagagtg tagaaataaa ttcaaggtta attttataat aaatagtaac
    16381 attgaatttt ttgaaaaagt ggattgtcaa ggtatccatc ttccttttaa attattcttg
    16441 aatttaattg aaaataagta taattttaat gaaaataaaa tactaggatt atctttacat
    16501 aaagtagagg aagtagatta tttagaaaaa ttaataagaa atcaaaatat aaaaatagat
    16561 tacataacat tatcacatat atatgaaact aaatgtaaag aaggattaaa tccaaaagga
    16621 attgagcttt taaaagaagc taaaaagatt actgatatta aaataattgc acttggggga
    16681 attttaccat ctaatgtcaa agaaacatta aaatactgcg atgattttgc tataatgtca
    16741 actataatga ggtctaaaga tattaaaaaa accatatcaa attataatga aaagttgaat
    16801 tagtaaatat agtatattga atttttaact taaataaaaa taaaaaagtt atcttttaaa
    16861 ttttaaaagg tagctttttt attttatatc tttgctaata aaaaattgta aaaagggaaa
    16921 actttagtca gagagattaa acaatatcct agaaagattt aaaatttagt aaagtatgga
    16981 ggacattata atgactgaca gtaaaaagaa acgatttatt ataccacaca cttttacaat
    17041 aatattttta ctaatagtct tgatggcaat attgacttgg atagtaccaa gtggtgaatt
    17101 tagtagagaa gatgtagaag gtagaatggt agttgtacca gggacttatc aaagtgtaga
    17161 aagtaatcct caaggatttg cagatgtttt taaagcacca atagaaggct ttatagattc
    17221 agctgaagtt gtaggttttg ttttaatagt tggaggtgct tttggtattg taaataggac
    17281 tggagctata gaagcaggaa tagcttcggt tatctctaag tttaagggaa aagaacttct
    17341 tataattcca atatcaatga ttttatttgg acttggaggt acaacatttg ggatggcaga
    17401 agagacatta ccattttata tgatatttgt tccactcatg acatccttgg gatatgattc
    17461 tctaacagca atagcaattg tatttatagg agcagcagca ggagccgccg cttcaacaat
    17521 aaatccattt tcagttggta ttgcacaggc actagctcaa cttgctcctg gttcaggagt
    17581 aacttacaga gtagttattt ttatagcata tatactaata agtatagcat ttgttatgta
    17641 ctatgctaag aaggtaaagg aaaatcctga aagttcaata gtatatgaaa tagataaaaa
    17701 taataaatca atacttaatc aaacttcaac agaaataaaa gaatttactt ttaaagaagt
    17761 atctgttctt gctatatttg gaatagggat ggcaataatg atttggggtg ttttaagtca
    17821 aggatggtac attccagaaa tatcaatgtg ttttgctgca ataggtgtgc tttctggatt
    17881 ggttggtaga ttatctcaaa ttgaaatctc agaaagtttt atagatggag caaaagattt
    17941 agcatctgca gctcttgcta tagctttggc tcgtggaatc gtaataattg ctcaaaatgg
    18001 atttataata gatactatat taaattccgc agcagccttt ctaggtgggc ttccaaaggt
    18061 tatatttgta tatttatcat ttttcttgga agctgcattg gcatttttaa ttccatcatc
    18121 ttcaggtctt gcttcactta cagtacctgt acttgctcct cttggtgatt tggttaatat
    18181 atcggctcaa gtgatagtaa ccgcttatca atttggtgtg ggtcttacaa actttataac
    18241 tccaacttca ggagtactga tgggagcact tgcagtagcc catattccat tttcaaaatg
    18301 ggtaagattt ataatgccta tcttgttgat tttgacagct gtatctctag tctttttaac
    18361 aataggtatt tatatagggt tttaattaag tgtagtttta tttaagatat ttagtaatgg
    18421 tataaaatga taatatgttt tcaataaatt gccactaatt taattagtgg taatt
//
LOCUS       DAELXX010000082        18461 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.082, whole genome shotgun sequence.
ACCESSION   DAELXX010000082 DAELXX010000000
VERSION     DAELXX010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18461
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.082"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..790
                     /locus_tag="KPH29_002631"
     CDS             <1..790
                     /locus_tag="KPH29_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG1468217.1"
                     /translation="EIDSYLIDGQSFEENLIKDIIENLKIKNNSDIETYKQSLKELDD
                     YIAKWVHEIKIPISSLSIITDRLSSIEDSLDIKNQVAKINFLVNSILYSSRSNTMFED
                     VFINKFNLEKLVKMSIKNNSFLLIKNNVEVSLNELENDVYTDSKCMSYVLDQIINNAI
                     KYSKEVGKIEFNSKKLENGVVLSIKDFGIGINEEDISRVFDKGFTGKNGRNQLYKSTG
                     MGMYFVKKMIDSLGHEIEVCSKIGSYTIFNIYFYDISDYLSLDS"
     gene            1220..2380
                     /locus_tag="KPH29_002632"
     CDS             1220..2380
                     /locus_tag="KPH29_002632"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBG1468218.1"
                     /translation="MITMYSVGLMSGTSLDGVDAVLAEISGNGRNTKVKQIEFITLEI
                     PKDIKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHG
                     QTIFHIPRSYNNFVSSTLQIGEPAIIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEF
                     LLYSDKNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYD
                     KNGYFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDL
                     IATVTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGVHNLTLIGYIKELLLEVEVLTQD
                     EYGYSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            2382..2771
                     /locus_tag="KPH29_002633"
     CDS             2382..2771
                     /locus_tag="KPH29_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468219.1"
                     /translation="MSNNKKKKRKKNNNYKNAKSNKNTKKEMEKNQRTDYNIPTISFK
                     SGLILIVSSLVSTVVFPLLLSIFGIDNRFGVVIGNSLITSFAVAYTRYFVETKRGFSL
                     GFFRLYILFVLSFAIIGYFWIYKGIHI"
     gene            3032..3340
                     /locus_tag="KPH29_002634"
     CDS             3032..3340
                     /locus_tag="KPH29_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG1468220.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFENE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEASVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            3429..4775
                     /locus_tag="KPH29_002635"
     CDS             3429..4775
                     /locus_tag="KPH29_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG1468221.1"
                     /translation="MLDKFTEFLDSKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPDLLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            5008..6105
                     /locus_tag="KPH29_002636"
     CDS             5008..6105
                     /locus_tag="KPH29_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBG1468222.1"
                     /translation="MGRLGISIYPEKTTSKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKSINEYAKFKGFEVILDVNPRVFGELEISYKDLTFFKKTKADGIRLDMGFT
                     GSEKALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLTGCHNFYPHRYSGLNLEH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFDFKPSTK"
     gene            6204..7118
                     /gene="murQ"
                     /locus_tag="KPH29_002637"
     CDS             6204..7118
                     /gene="murQ"
                     /locus_tag="KPH29_002637"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBG1468223.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNRVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            7228..8148
                     /locus_tag="KPH29_002638"
     CDS             7228..8148
                     /locus_tag="KPH29_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HBG1468224.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDGIQGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLEHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPDLGACLLAKKKYYSK"
     gene            8165..9016
                     /locus_tag="KPH29_002639"
     CDS             8165..9016
                     /locus_tag="KPH29_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG1468225.1"
                     /translation="MSCILKLKQIFEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
     gene            9628..10659
                     /locus_tag="KPH29_002640"
     CDS             9628..10659
                     /locus_tag="KPH29_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBG1468226.1"
                     /translation="MKKIRKLATKLLITTIVLVSGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNNKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPTVGAVAQTQKPALPGKHTMLPHQDGAK
                     VVGKFGDIKDKHVMYEGVRCVVINSSVNTVTFLGSKSNLDPSNLTIAVIGFMNTPYGM
                     FALAPEGEPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIEITDEKANIGL
                     GQIMYIRYE"
     gene            complement(10775..11749)
                     /locus_tag="KPH29_002641"
     CDS             complement(10775..11749)
                     /locus_tag="KPH29_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468227.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYKLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     LSMKNHPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQMLRDNNFYK"
     gene            12205..13086
                     /locus_tag="KPH29_002642"
     CDS             12205..13086
                     /locus_tag="KPH29_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HBG1468228.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEVDQKALLDAV
                     NTGDYSNKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNAND"
     gene            13468..14271
                     /locus_tag="KPH29_002643"
     CDS             13468..14271
                     /locus_tag="KPH29_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468229.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESVLKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            14283..15413
                     /locus_tag="KPH29_002644"
     CDS             14283..15413
                     /locus_tag="KPH29_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG1468230.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISKSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLRESIDEPIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEGFVKSYFESYSLIRG"
     gene            15417..16826
                     /locus_tag="KPH29_002645"
     CDS             15417..16826
                     /locus_tag="KPH29_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468231.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDMDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYVIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDVEIDYSP
                     ERTHDIWDESNEIDKNTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPWNLYL
                     NRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKAEIAVALDVSGSISDEEFKQAI
                     KEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            17037..17546
                     /locus_tag="KPH29_002646"
     CDS             17037..17546
                     /locus_tag="KPH29_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBG1468232.1"
                     /translation="MVMFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPI
                     YSNNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRG
                     GKCEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLSEEFATILDISPNEVS
                     SYISSHIPQ"
BASE COUNT         7376 a         1892 c         3220 g         5973 t
ORIGIN      
        1 tgaaatagac agttatttaa ttgatggaca atcttttgaa gaaaatctaa taaaagatat
       61 tatagaaaac ttaaaaataa aaaataatag tgacatagag acttataaac aatcattaaa
      121 agaattagat gattacatag ctaaatgggt tcatgaaata aaaattccaa tatcttcttt
      181 aagtataatt acagataggt taagtagcat agaagatagt ttggatatta agaatcaggt
      241 tgcaaagata aattttttag taaactctat tttgtatagt agtagaagca acactatgtt
      301 tgaagatgta tttataaata aatttaattt agaaaaattg gtaaaaatgt ctataaaaaa
      361 taattcattt ttgcttataa aaaataatgt agaagttagt ttgaatgaat tagaaaatga
      421 tgtttacaca gattctaaat gcatgtcata tgttttagac caaattatta ataatgctat
      481 aaagtattca aaggaagttg gtaagattga atttaattca aaaaagttag aaaatggagt
      541 ggtattatct ataaaagatt ttggtatagg tataaatgag gaagatatat ctagggtttt
      601 tgacaaggga tttacgggga aaaatggaag aaatcaattg tataagtcta ctggaatggg
      661 gatgtatttt gtaaagaaaa tgatagatag tttagggcat gagattgagg tatgttcgaa
      721 aattggttca tatacaattt ttaatatcta cttttatgat atatcggatt atttaagcct
      781 agatagttag gttattagac tataaaatct atcatcatat acaaatcgac atatacaata
      841 agaataaatt tagttcttga aaatagtgaa gatttaatta gtgccttaaa tgatgttcta
      901 tctatattat aaaataataa ttattatata tgaaaaacat atcagatagg tacctggtat
      961 gttttttatt tgtagtaact attttaatat ggtttttgga gagagaatat gttttgtttg
     1021 tgaaaaaaaa ttacatgcat tacattgaca ctgaaaataa attaatgtaa gatttagcta
     1081 aggtaaaaaa cgtgtataag attaactcga ttaatttcgt aagagtaaat tatcgcaatt
     1141 aaagatgaaa taagattaat tctaaaatta ttaaacttaa taccttgatt tggggaggaa
     1201 taactataaa cttaaaagga tgataactat gtatagtgta ggtttaatgt caggaacttc
     1261 cttagatgga gttgatgcag tactagctga aattagtggg aatggtagaa atactaaagt
     1321 taagcaaata gaatttataa ctctagaaat tcctaaagat ataaaagacg aaataagaaa
     1381 gtgctgtata gaagaggaat cgagtgttga tttaatctgt agcttaaatt ttaagcttgg
     1441 atatttattt agtaaagctg taaaatcagt gtgtcataaa gcaaactttc atatagcaaa
     1501 tttggatttt atcgcatctc atggtcaaac aatctttcat ataccaagaa gttataataa
     1561 ttttgtatct tcaacgcttc aaataggtga accagcaatt atagcatatg aaacaaatac
     1621 aaaggtaata tctaatttta gagtaatgga tatagcagca ggtggagaag gtgctccatt
     1681 agtaccatat agtgaatttt tattatatag tgataaaaat aaaaatttag cacttcaaaa
     1741 tattggtgga ataggaaata ttactataat acctaaatca tgtaacatag atgatgtatt
     1801 tgcatttgat acaggaccag gaaatatgat aatagatgga gtatgtcaaa gattatttaa
     1861 tagaaagtat gataaaaatg gatactttgc atcaaaaggt aaaattaatg aagaaatgtt
     1921 aaaagatttg atgagtcata agtatcttag tcaagcgcca cctaaaagca ctggaagaga
     1981 ggtatttgga caagtctatt tggataacat gcttaacaaa tataaacatg tagataaata
     2041 tgatttgata gcaacagtaa ctatgtttac ggcaaaagct atatattata attataaaaa
     2101 cttcatactt ccaaaggtta atgtagatac tttattaatt ggaggtggag gagttcataa
     2161 tctaacttta ataggatata taaaagagtt attattagag gttgaagtat taactcaaga
     2221 tgaatatggg tattcttctg atgcaaagga ggctttggca tttgtaatct tgggcaatga
     2281 aacattaaat aattcttttt ctaatgtaat atctgcaaca ggggctaaaa ataaagttat
     2341 tttagggaat ataacaccta aaccttttgg aggtaaatga catgtctaat aataaaaaaa
     2401 agaaaagaaa gaaaaataat aattataaaa atgcaaaaag taataaaaat actaagaaag
     2461 aaatggagaa aaatcaaaga acagattata atattccaac tataagtttt aaaagtggat
     2521 taatactaat agtttcatct ctagtaagta ctgtagtttt tcctttgtta ttatctatat
     2581 ttggaataga taatagattt ggagttgtta taggaaattc attaattaca tcttttgcag
     2641 ttgcatatac aagatacttt gttgaaacta agagaggttt ttctttggga ttttttagac
     2701 tgtatatttt atttgtttta tcatttgcaa taataggtta cttctggata tataaaggaa
     2761 tacatattta aaaaagtatt gagactattt tatgttaata aaagatagtc ttttttcttt
     2821 tttaagaaaa gatatgagta ttttaagtag tgtgtaattt tagacaaaag aatgattaga
     2881 taaataaagc aattaaagca agatataaat gcatgaaata aaattacaat taaaaatgta
     2941 aaatatgaaa aaatatatac aaacgtattc ctgtaatcaa aaatacatgc tataatgatt
     3001 gaaaagaaat aaaaaaacag ggggaatcaa tatgagagta ttatttgttt gttcatcagg
     3061 gatgtcaagt gctatagcag ttaatgcatt acaaaaagaa ggagcaaaaa atggaataga
     3121 tatagatgtt ttggctgttg gtactcaaga gtttgagaat gaagttaaaa atggatggga
     3181 tattgctatg gtagctccac aagtcagaca tagatttgac tatttaaaag cttttgctga
     3241 tgaggctagt gtaccatgtg cattaataca agcacaagct tatagtccat taggaggacc
     3301 aaagttatta aaacaagttc aagagttgtt gagtaaatag tagttgcagt gttgttaggt
     3361 tagaagaaag ttaattttat aaaaatttta atgataataa aaataaatat taattaagga
     3421 gagaaaagat gctagataaa tttacagaat ttttagacag taaattatca acttctatgg
     3481 ccagattggc agaacaacgt cacttaagag ctgtaagaga tggaatcgta gcttcattac
     3541 cacttataat agttgggtca tttttcttga tattagcatt tcctccatta ccagattcat
     3601 ggggggtaac tcagtatcta aaggaaaatg cagttacaat attactacca tatagaatga
     3661 cattttttat tatatcagta tattctacat ttggcatagg ttatagtttg gcaaagtcat
     3721 ataagttaga tggattgaca ggaggtttat tagctacagc tacattcctt ttgacaatag
     3781 ttcctactgc aatagagggt cttacccctg atttattaaa gatagttgaa aagagtccag
     3841 aacttttagc ttggtataat tcaattcctc aaggatggca aatgccaatg gctaatcttg
     3901 gtggaggttc aatgtttgtt gctattgtaa catctataat aggtgttgag atttatagat
     3961 ttactgacaa atctaaattc aagataacca tgccagaagc agttccagca tcagtcacaa
     4021 ggtcttttga agctctgaca ccagccgcat taataataat tttaatggct attgttactt
     4081 atttcctagg gtttgattgg catggcttca tagctaaatt agtaactcca ttagtttcag
     4141 ccactgacac attaccatca gtaattatac taatattttt aataacgttt ttttggtcat
     4201 ttggaataca tggtgtatca ataataggtt cattggcaag acctttatgg atacaattat
     4261 tagatgctaa tacagcttca tatgctgcgg gtcaagcagt tccaggtatt gctgctgaac
     4321 ccttttttca atggtttata tggattggag gttctggagc tacaataggc ttagctttac
     4381 ttcttgcatt tagagcaaaa tcagcttatg gaaagtcttt aggaaaagca actgtaatac
     4441 cagcaatatt caatataaat gaaccaataa tatttggagc accaatagta ttaaacccaa
     4501 tattaataat accatttgtt gtggcaccaa tagtaatggc aacagtttca tggtttgcta
     4561 tgagtgctaa tttagtatcc agagtagtag ttactgctcc ttggacatta ccaggaccag
     4621 taggggcata tttagccact ggtggagatt ggagagcttc agttcttaac ttattatgta
     4681 tagctatatc aataataatt tactatccat ttgtcaagat gtacgataag agattgcttc
     4741 atgaagaaaa agttgaagag caagaagctt attaattaga aactatattg gaaattaaaa
     4801 actatattag attagtataa atttcaaagt ataacagtta tgaaataagt aacgaagata
     4861 ttttgttgat tgtttataaa aaaagaaaag gtaaactttt ctttttttat aaatatatat
     4921 gaaacttttt ttcaccaaat ttttaaaaaa gtttcgtgta attcgaaata aatttaaaca
     4981 catataggat ataaggagat gacacagatg ggaagattag gaatatcaat ttatccagaa
     5041 aaaacaacat ctaaggaaat atatgattat atagataaag ctgctgaaaa tggatttaca
     5101 cgtatatttt catgtttatt atcagttaaa gatactaaag aaaatataat taaaaaattt
     5161 aaaagtataa atgaatacgc taagtttaaa ggttttgagg taatacttga tgtaaatccc
     5221 agagtatttg gtgaattaga aataagctac aaagatttga ctttttttaa aaagacgaag
     5281 gcagacggaa taaggcttga tatgggattt acaggttctg aaaaggcctt aatgactttt
     5341 aatcctcaaa atcttaaaat agaaatcaat atgagtaata atactaatta tatagataca
     5401 ataatggact accaaccaaa taaagaaaat ttgacaggat gtcataactt ttatccacat
     5461 agatatagtg gattaaattt agaacacttt ataaaatgta cagaaagatt taataagtat
     5521 ggattaagaa cagcagtttt tgtaacaagt caaaatgaaa ccacatttgg accatggcct
     5581 gtaactgatg ggcttccaac aattgagatg cacagaaatc tacctataga tattcaagta
     5641 aagcatataa tagctatgaa taatataaat gatataataa tatcaaattg ttatccaaca
     5701 tatgaggagc taaaggtatt aggttctatg agaaaagata tggttacatt tgatgtagca
     5761 atagaggaaa atatacgaga agttgagaag aagatactct ttgaagaaat acattttaat
     5821 agaggggata tatctgacag tctgataagg tctactcaat ctcgtgttaa atataaagga
     5881 tacaaatttg aactatttaa tactcctaaa ataataaaaa gaggagatat agtaattgaa
     5941 agtagtgaat atggtcatta tgcaggggag ttacaaatag ccttgactga tatgaaaaac
     6001 agtggtaaat cgaatgtagt aggatatata agaaaagaag aattatttat attagattat
     6061 attaaaccat ggcaaaagtt tgattttaaa ccctcaacaa aataatttat tatgaaaata
     6121 ttaatttatt ataaaaatat aattatgaaa gttggagtta aaatgtgttt aaaaatttaa
     6181 tattaagaaa atgaggaaat gatatgttaa taaaaacttt agaaaattta gttacagaag
     6241 gtagaaataa gaatacattg caaatagata aagaagatac attaggcata atagaattaa
     6301 taaataacga agataaaact gtagcctatg ctgttgaaga acaaaaggag agtatagcaa
     6361 aagctgttaa tataatagta gatagaatga agcagggagg tagattattc tatataggag
     6421 caggtaccag tgggaggata ggaatactag atgccacaga atgtcctcca acttacggag
     6481 tggattttga gcttgttcaa gctataatag ctggtggaaa tcaagcaata tttaaagcaa
     6541 tagaaggtgc tgaagatgat aaagaacttg gaaagcaaga tataatagat agaggagtta
     6601 catctaagga tgttatatgt ggtatagctg catcaggaag gactccttat gttataggtg
     6661 caatggagta tgctaaggaa ttggggtgtg cagtactatc aataactatg aatccgaata
     6721 gtgaaatgag taaaaaagcg gatttaccaa taaacattat agtaggggca gaagtaataa
     6781 tgggttcaac tagaatgaaa agtggtacag ctcaaaaaat ggtatgtaat atgttaacaa
     6841 cagcttctat ggttaagatg ggaaaagtat actcaaactt gatggtagat gttaagacat
     6901 caaatgaaaa gttggttgaa agagcaaaaa gaataataat gatagctaca aatgtaaaat
     6961 atgacgttgc agaaaaattc ttagaagaag cagataatag agttaaattg gctatattta
     7021 tgataaaaag tggtttagat aaggatagtg caaagagtat cctagataga caagaaggtt
     7081 atataagtga agctttaaaa tcaatagaaa aattgtaaaa taaataatag tactgataac
     7141 ctttcaatat taaaaaaaca gcattggaag gttattttta gatattaagt attgaaagaa
     7201 ttattaaaga tattgtagga ggatagtatg tattttttag gtgtagatgg tggaggaacg
     7261 aaaacaacat ttacattagt agatgaagaa ttaaatatag taggaaccat aaccaaagga
     7321 acttgtcatt ataatcaaat aggatttgat aatctaacga aattattaat aactgggttg
     7381 gaagaagttt gtaaagatgc taagataaat gtagaagaaa taacgtatgc atttgttgga
     7441 cttgctggat atggaaaaat aaaagaagta ttgtatgcat tagaagtagc aacaaaaaat
     7501 gcatatagtc atataaatta tactctaggt aatgatgtag aaatagcatt agcaggttct
     7561 ttaaatgggg aaaaaggtat aaatataatt gcaggtactg gttcaatagc acaggcttta
     7621 gataaagatg ggaatcttca cagatgtgga ggatggggct atgtattagg agatgaaggt
     7681 tcagcgtatt atataggaat ggctacctta aaaatgttca caatgcagtc tgatggaagg
     7741 tgttcaaaga caaaattata tgatttaata aaaagacatc ttaatataga gaatgattat
     7801 gacataataa aatatgtaaa tgatggaata caaggagata gaatagaaat agcaaagttt
     7861 gctacgatat gtttaaaagc agttattgaa ggtgataata ctgctagtaa aatatttgat
     7921 gatgcagctt atgaattaag taggcttata ataggattag agcatcattt tgaacaaggt
     7981 acaaagataa aggtatctta ttcaggtgga gtgtttaagt caggagattt aatattagag
     8041 ccacttaaaa aatatttgaa taaaacgaga tttggtatag tgagaccagt gttgactcca
     8101 gatttaggag catgtttact tgctaagaag aaatattata gtaaataggt agaggtggta
     8161 gagtatgagt tgcattttaa aattaaaaca gatatttgaa gatttaacgg aagtagataa
     8221 aaaaatagct gattacatac ttaataatac agaggctata agtaagcttt ctgtatcaga
     8281 attggcgtca aactcaaaaa caagtacagc aagtatagtt aggttttcta gaaaaatggg
     8341 atattcgggt tttggggatt taaaaattga gatagctaaa gatttgatgg gcaaagaaaa
     8401 tgaatacaca tatgtagatg aaaactatga ttgtaatata gatagtgtta taaacaaaat
     8461 tacaaacaaa aacatagaga ctatcaacca aacaaggttg ttgaatgaaa aagatataat
     8521 aaaagaagca gttgaacaga taataaaggc taaaaatgta tatatatttg gagtaggtgg
     8581 ttcggcttta gttgcactag atttacagat gaaactttta aggataaata aacaggcatt
     8641 tacctcttta gatagtcata cacaacttat ggtatcttca aatgtagata aagaagatat
     8701 agctatagca atatcttact ctggagaatc aaaagaagta ataaagtcta tagaaaatgc
     8761 aaaattaaaa ggatgtaagg tcatttgtat aactaaatat agtgacaatc atcttagtaa
     8821 gattagtgat ttaaagctag tagtaccaaa tatagaaaaa aggcttagag aaggagctat
     8881 ttcttcaaga atagcaatgc ttacgttgat agatataata tatatttcta taattcaaga
     8941 aaacttaaat gaggcagaac agaaactaca agaaacaaaa aggatacttg attatttaaa
     9001 tattaataat gcttaaaact aggttatata tcaactaaac aaataagtta gactataagc
     9061 gtaagtcaat aagaaataga taaatatcta aaatattgac ttacttaata ataatatata
     9121 gcatttttat aatacaaatg agttgtctta aaattgattt tttttaatca aattttaagt
     9181 tcaactcctt ttttatattt ttatagatat atcctatatt ttcccagaaa acgtattgaa
     9241 tatacaaatt ataagaagta taatgttgtt gggagagaat gtatgaaatg tatacaaagt
     9301 tgattttaac tgtattattg ataattctct ttactcttac cattattaat gattgaagac
     9361 aatggtattt attataggtc aaagattccg aatatattaa tcatcatcat tttctcaaac
     9421 taacagcaaa caccctgatt tgcgagttgt attgtataca aattatctag aaaatatcta
     9481 ttagattttc catgaaattt aaatatttca cgaacgtata aatgaagaaa catacatatt
     9541 gacaatccac ctttttaaat cccaaatgaa ctttattaat ggtagagaat aggaagtaac
     9601 aatattttat aaaggagaat actaagtatg aagaaaatta ggaagttagc taccaagtta
     9661 ttgattacaa caattgtttt agtctcaggt atgagtatga cagtttatgg tatgacagcc
     9721 aaagaggtaa cagcaaaaac accaaaaagc tatgtcactg gaaccaatag cgtatatggt
     9781 ccaaagctgt cacaagccca actaaactca gtagcacaag caacagcaga ttttatgaac
     9841 aataaaatta caaagaacat gactacagac gctaagattt tagttgccta taatcacatt
     9901 aaaaataaca caacatatgt tgactggaat gcagtagaag gagcgaatac tgcctataca
     9961 ttggttacta aaaaaggtgc atgttcagga atggctagga gcatgaaagc tttgtgtgac
    10021 gctatgggaa ttgaatctta ttatgtacac agcacatcaa atgaccacca gtggaatctt
    10081 atacgttttg gtgatgggcg tttgtatcat gtagatattg atgctaataa atcagcagga
    10141 aaggatatca tatacaagag tcttagccac ccattaccat ttgataagac agcatatcca
    10201 acagttggag cagtagcaca aacgcaaaaa ccagcactac ctgggaagca cactatgtta
    10261 ccacatcaag acggggcaaa ggttgttgga aaatttggag atataaagga taaacatgta
    10321 atgtatgaag gtgttagatg tgttgtaatt aattcatcag tgaatacagt tacatttcta
    10381 ggtagtaaat caaatttaga cccatcaaat cttacaattg ctgtaatagg atttatgaac
    10441 acaccatatg ggatgtttgc attagcacca gaaggtgaac ctgttgaata tccttttaaa
    10501 tatgataagc cattttcttt agattttagt ggtaaaaatc gttctggtgc agaattagca
    10561 aagtactgta aaaaaaatga tgtgactatt aaaatagaaa tcactgatga aaaagctaat
    10621 attggacttg gtcaaataat gtatattcga tatgaataat gatttaatca tgacagtaga
    10681 ataagttata aacatcgcgc cctcttacta ggggcgcatt taatattata tctgatattt
    10741 tgtagtcagg tcaactgctg gatttctcac ctttttattt ataaaaatta ttatctctaa
    10801 gcatctgagc atgtttacga ttaactaaaa attgttttaa ctcagtcaag cataggaata
    10861 aaatagctct gttttcaaac tctgttctgt caacaggtag aggatggttt ttcatactaa
    10921 gcattaatct cttcaattct tcaagaagaa gattgcctgt ttcagcttca aattcagtat
    10981 aacctatttt atgtatgaaa gcagatagaa tgtgagcttg tggtggtgta gaatttagtt
    11041 gagatacata actatacaaa ttttctaaga tatcccgttg gctttttata atatccatat
    11101 gttctaagaa gaatctagta tcacttaata aagtattatt aatattatcg taagtttcag
    11161 aaatactgct gtcaataagt gaatttaatt tatataaatc tctactatat ccattttctt
    11221 ttttaggatt tacaataata tctgcaatgt ctataagtac aatactcact tcttcctgta
    11281 actttttttg aacttcatgg attttatgta tatttgttgg aatatacata tttattaaga
    11341 cacctattcc agttcctact gcaaatattc cagcttcatt taaaatccat ttgatagaag
    11401 tttctccttg aaggtagtaa tgggatgcta tgactacaca catagcaata acatcttgaa
    11461 tatttaacaa aaaacaaagt ggtataatta taaatataaa tattgaaaat gaagtcaaat
    11521 tatatcctaa aatattaaaa cataaatatg aaaaaatagt acataaaaga aaaccataaa
    11581 tctttccaat ggagccttta agagtttctc ttttagtgtc ctttactgta agtagtacta
    11641 taactccagc aaccattgaa tattctaatt ttaatagcca agcaatgaat atagcaagag
    11701 aacatccaag agataatttt attactttaa gtgaattaat attattcaaa tgcaaacctc
    11761 ctgtaaatca atataataaa attctaacat aaaactaata ttgcttgaaa ggtaagtaac
    11821 tatacttaat tattttattg ccctatttgt ttagatacaa tcaatataat tttattttat
    11881 gaaaattttt tgtaaaattt taaatttaga gactaacatg agaccacaaa gaggaagttt
    11941 attataagaa gaaaacttaa aattgatttt aaatagcaaa tgaaaataat caaatatgta
    12001 tatactatta ttaaaaaata aaattatata gttaatataa ttttataatt ggtttagtat
    12061 agagattttt aacttttatg ttatactatt tataaaagta ggtataaaga ttaaaagaca
    12121 taattaaata cgtatgagag gttttaaata gtattgttaa tgaacttgat tattgtttat
    12181 aattacaaat tggaggagat aaatatgaaa attactgaag gatatatgcc ttttaaaggt
    12241 tttaaaacgt attaccgtat agtaggagaa aatacggaag ggaagaagcc attagtatta
    12301 cttcatggag gtccaggttc tacacacaac tattttgagg tattggataa gatagctgaa
    12361 agtggtagac aagtaataat gtatgaccaa attggttgtg gaaattcctt tgtagaaggg
    12421 catcctgagt tatttaatgc agatacttgg atagaggagc ttattgaact aagaaaacat
    12481 ctaggacttg atgaaattca tctgttaggt caatcttggg gaggtatgca ggctatttgg
    12541 tatgctattg aatataaacc aaaaggtatt aagtcttaca tcctttcatc tactctttct
    12601 tctgcaaaac tttgggagaa agaacaaaaa agaagaatat catatatgag tgaggttgac
    12661 caaaaagcat tgcttgatgc agtaaatact ggagattact ctaataaaga atataatgat
    12721 gcattagaaa gatttatgga aatgtattgt gcaggtgaag tgacagaaga ttctccagag
    12781 tgcctgagaa gacctaaaaa atcaggttct gaagcatata ttgtaggatg gggacaaaat
    12841 gaattttctc ctacaggaac tctttctgga tatgaattta cagatagatt acatgaaata
    12901 aaagagcctt gtctagtaac tagtggagca atagatttat gttcgccata tattgcaaag
    12961 actatgtatg atagaatacc taatagtaag tgggagttat ttgagtattc aagacatatg
    13021 ccattcgttg aagaaaatga gaaatatata aaagtcttaa ctgagtggtt gaatgcgaac
    13081 gattaaaaac tatataaatt tatataaaga aatgagtgtc tcaaaatgaa ctttttagtt
    13141 cattaggcac tctttttgca tgaaatcaaa tatattttca ttatttgaac gataaaatga
    13201 ggcttatatc tattttggga aagtaatttt ttattactag cgttagattc taatctaatt
    13261 gtatacttat tgaaataata gaaatatatt ggtgtttata acttaatttt attgtttttt
    13321 agaaattttt ttatatattg gtataattat tctgtttaaa tttgattaaa aagtatatta
    13381 attataataa gaaacaatta tataaatagg aatagattat tataattaat atagatataa
    13441 aaataattat agaaagaaag gcacattatg gaaaaattga gagttattga aatgataaaa
    13501 aataatccca atattatagc tacaatagat aatccaactg atgaaatgaa attgttagct
    13561 attaaagaaa atgggcttgt actagaatat ataaataatc caaccagaga gatgcaagaa
    13621 ctagcaatag ataataatat tagagcaatt aaatttatag ataatccaac agaagatatg
    13681 atgataaaag cagtaaatga gggatggagt atactggatt atatcaaaaa cccaactgat
    13741 aaagttattg aaatggctat aaatcaagca ggatgggcaa ttaaatatgc taaaaatcca
    13801 agtgaagaat tacaactgtt agcagtaaga aaaaattatg attcgataaa gtttataaaa
    13861 caaccttatg aaagtgtaca agaggaggca gtaagaataa gttatgatgc cttgagatat
    13921 atagattcac ctagttataa tgcagaactt atagctataa aaaataatga agcagctatt
    13981 agctttataa ctgatttaga taaaaataaa atgttggaat ttttaaaagt gaatatttta
    14041 gtgattaagt acattctgaa aaaagtatct aaagatgaat tagagagtgt attaaaagaa
    14101 gtattatcca aagaagatgt tgaggaaaag tatattagag attttttgaa ttgtagtgtt
    14161 atagatagaa atagcgaaag tataccaata gataaaatta tgtttattta taaatatgga
    14221 agtaagacag caaaaaaaat agctgtagat gagaaattaa agattatgta ggagagagaa
    14281 aaatgaattt tatagacaca ttaaaaagcg ttgacttaat attatccact ggagaagtac
    14341 ctttaatagt aggtgaaagt ggaataggaa aaacagcttt agcaaataag cttgccaaag
    14401 aaaatgattg gagtttaatt gttattgatg gaaacttact taaagagggt gaaataggtg
    14461 gtcttccaac tatagaatct tatgtaggag ttaattctaa tggatataag actgaaaaga
    14521 aaacaacagt gtatgctgtt cataacaaat taagagaaat tgatgaagag atatctaaaa
    14581 gtaagacagt tcttttattt atagatgaga taaatcgttg tgagcataca gtacagcaag
    14641 aacttatgaa tttaatttta aatagagaaa ttaatggata taaactgcat gatgatgtaa
    14701 agattttagc agcaatgaat ccatcaagta aatatggttc agattttgat tatcaagtgg
    14761 tagatatgga tgcagcccaa gaaaataggt ttgtttggtt aaatatggag agtgaccata
    14821 cacaatggat aaaatgggca atagatgagg gtattgaaag aaaagttatc gagtttattt
    14881 ccacttttcc agaatatctg cataaaataa atgaagatga tgtaagagct actcctagaa
    14941 gttatgagag agtatctaaa atttataaag tttataaaga aaaaaataat tctattccta
    15001 gagcagtatt tttaaatgtt gtaaagggta atgtaggtaa agttattgca gaagaattta
    15061 taagttttat tgaatcaaac tctgaaccat taatatccta tgaagatgtt tttttaagag
    15121 aatctattga tgagcccatt gtagaaagag taaaaaatga gagtcataca agactttatc
    15181 tatcagctat gaacattcta aaagacttag aattaaatat taagaatgat aaatatgaat
    15241 caaaccacta tattaataga tttattgaat ttttaaaaat gtatcctgta gatttaatga
    15301 taggtattat gaaggatatt agaaacagtt atattgaagt atataaaaaa gccatagaaa
    15361 atgaaggttt tgtaaaatca tattttgaat cttatagctt aataagggga taatgtatgg
    15421 aaaattattt tgataaacaa gcaaaagagc tttataataa agcaagtgaa attataaata
    15481 ctcattctat gctaaaggca aatcgtgcta atgagaaatt tgatatggat ataccacaag
    15541 attttaagta tgagtttttt agtcttgtag ataaagtgaa tttaagtctt atggaagaag
    15601 aagataattt ttatggatat tttttgtttc agatgtcaag agaaataaga tttgatataa
    15661 gtagtcctac aggcgtgaat tttaagggag ctaaatatgt tatatatttt aatccaataa
    15721 tctttttaac gcttgacata aagcaaatgg agactacaat taaacatgaa attcatcata
    15781 tattatctat gcatttgatg agagcaaagg agttaaaagg aaagtatagt acattggcaa
    15841 ttaatatggc aatggatata gtagtaaata aatttctaaa caatttacca ccatatgcaa
    15901 ctaccttaga gtgggtaaat ttaaaatatt ccttaaaact tgaaccatat gaaccttttg
    15961 aatattatgt agaaaagatt cagactgaat tagatttgat ggaagtggat gaagatggtg
    16021 aggaagatga tagtgataaa tatggagatg tagaaattga ttatagcccc gaaagaactc
    16081 atgatatatg ggatgaatcc aatgagatag ataaaaatac ccttagagaa tttactgaaa
    16141 aatttattaa cagttctcaa aaaggtaaag ttcctgctta tttagaaagt atgatatcat
    16201 cacttaaaaa tagtaagggt gaattgccat ggaatttata tcttaataga ataatgggaa
    16261 cagttgaaaa taataaaaag aagactataa caagaagaaa tagaagacaa ccaaatcgtt
    16321 tagatttgag gggagaactt agaagtcata aggcagaaat agcagttgca cttgatgtaa
    16381 gtggaagtat tagtgatgaa gaatttaaac aagctattaa agaagttctt agtatagtga
    16441 aaaactataa tcatgaaatt acaattatag aatgtgataa tgaaattaga cgtgtatata
    16501 aagtgagaac tgaaaaagat ataaaagata gaattaggat aggtggaggt actagatttt
    16561 ctccagtctt tgagtatgct aataataaaa agattaattt gttagtctac tttactgatg
    16621 gtaaaggtga aaataaacta caagtaatac ctagagggta taaaatttta tgggttattt
    16681 ctggaagagg agataaaatt tcattaaaag aaccttatgg agcagttaaa aaacttacta
    16741 aggttaaaac aaatgatgaa atattagaaa taaatgatgt aagaaatgat gggtactcaa
    16801 tgaatagcca agcaccaatt ttataggtat tcacaggaga taagctgtat taatttattt
    16861 tgatatgctt gttataaaaa tctaaaaaat acattaatca atggttaatg tattttttaa
    16921 ttttaataat ataagtttta cttacattta agtcttaaaa tgtgttaaaa tataatatat
    16981 agcagatagt tttggcaaga tggatttagt tgtcaaaact atttttttta ttaaggatgg
    17041 tgatgttttt gtttaagatt gatgattata taatgtatgg tatgacagga gtctgcaaag
    17101 tcatggatat aacaaatgaa cgatttacaa atggaataaa aaaagagtat tatgtattga
    17161 gtcctattta ttctaataat acagtaataa aaattcctgt agataatgaa aaagtaccaa
    17221 tgagaaagtt actttctaaa attaatgtgc tttcattaat aaatgatatt cctaatatgg
    17281 atacatcatg gatagataat gaaaaattaa gaagtgagca atttaaaaaa atactcagag
    17341 gtggtaaatg tgaagagtta ataaaattag taagaagtat agataataac agagagtatg
    17401 taaaatctat tggtaaaaaa acacatcaag cagatgataa tataatgaaa gaagcagaaa
    17461 ggttattaag tgaagaattt gcaactattt tagatatttc tcctaatgaa gttagttcat
    17521 atatttcaag tcatatacct caataaagtg ttttagtatc ctttatttaa tctattttaa
    17581 gtattattta tgtatgctgg ttttggtaaa ataaaatata taattaaatt atataaaaaa
    17641 ttaagaatga ttgtattgaa gaattacaga atagtgcgga gagcccactc ctttaggtgt
    17701 gagatggata tcacttaaag atttaaattg ttattgtaga tgtttttaat ataaagcaag
    17761 attaataagt gtattgtaag ctttaagtga aattgatact ataatgagtt acttagattt
    17821 taaaatatat aatattataa aaaatccttt taggttttga atgaataaga tggatactat
    17881 gttgaataga atccatctta tttaagtttt atttatgttg tttatttgta agcattattt
    17941 ttgtacatga aaacacagtg taaaaatatg cttagaatta tgaagtttta ttaaaaatat
    18001 attcgacaaa ataagactaa aaatattctt aaacttataa ctaagataaa tattgtatat
    18061 ttatagtgta gcctgaaaaa ttgtggatgt acgaaattta tttagtattt tattgaaaca
    18121 aagtagaaga agtactatag tttatctttg aatagattgt tattttttag tgaaaaagtt
    18181 attaggttaa tacataaata taatagctta taaatagttt aagcatgtta aaaaagtagt
    18241 tattagagat atatagttac attgcttata agatacttaa aatgatagca ttcacttata
    18301 tttagaaaat taagataaaa aatcattttt atatagtaaa gcatacctaa atttagaaaa
    18361 taattgaatt tataaaatta attaaataca taaaaaagta ttcaattaac ttaatagatt
    18421 ttttaaaaat taatttaaat tttttcttct attaactatt a
//
LOCUS       DAELXX010000083        18215 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.083, whole genome shotgun sequence.
ACCESSION   DAELXX010000083 DAELXX010000000
VERSION     DAELXX010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18215
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.083"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(247..1344)
                     /locus_tag="KPH29_002647"
     CDS             complement(247..1344)
                     /locus_tag="KPH29_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBG1468233.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMDLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILFTYN
                     VKTYKPNFEWAEYPDLDILEKYIIENSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRN
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPEDCYKFLDILKSKLEIKKVI"
     gene            1637..3163
                     /gene="glpK"
                     /locus_tag="KPH29_002648"
     CDS             1637..3163
                     /gene="glpK"
                     /locus_tag="KPH29_002648"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBG1468234.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFESGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGVPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKEIKMLRVDGGITKNNFTMQFQSDILDIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTKQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            3204..4319
                     /locus_tag="KPH29_002649"
     CDS             3204..4319
                     /locus_tag="KPH29_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBG1468235.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGIIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLVFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(4600..5364)
                     /locus_tag="KPH29_002650"
     CDS             complement(4600..5364)
                     /locus_tag="KPH29_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBG1468236.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            5623..5713
                     /locus_tag="KPH29_002651"
     tRNA            5623..5713
                     /locus_tag="KPH29_002651"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5657..5659,aa:Ser,seq:gct)
     gene            5801..6589
                     /locus_tag="KPH29_002652"
     CDS             5801..6589
                     /locus_tag="KPH29_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HBG1468237.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHIGVLFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLAFKNKKPNNIENIIYFKEIATKARKEGLLSVDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEISE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            6610..7350
                     /locus_tag="KPH29_002653"
     CDS             6610..7350
                     /locus_tag="KPH29_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBG1468238.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDSLQNAKFKKVGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAVVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            7528..7902
                     /locus_tag="KPH29_002654"
     CDS             7528..7902
                     /locus_tag="KPH29_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468239.1"
                     /translation="MGNKNKNTKYKDKTRERYNKKVENLNKEKLNKDNKQSKKVDKID
                     DIINKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVK
                     ALFSIFVIVCFLIAFKYVVNPV"
     gene            7994..9454
                     /locus_tag="KPH29_002655"
     CDS             7994..9454
                     /locus_tag="KPH29_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBG1468240.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTIGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIEEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLNVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFETWFESFKAELENAIKK"
     gene            9507..12383
                     /locus_tag="KPH29_002656"
     CDS             9507..12383
                     /locus_tag="KPH29_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG1468241.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKILNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILNDKNINDKPIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQEGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRIEKLVIISI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKDKKLKWIRMNIFEDELKVLG
                     VVVDVTKEFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLD
                     NLKYINDTYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRD
                     IINSVKKQMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTI
                     KGEVSEFDINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPK
                     IKTFSSPIDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLS
                     KVDFEEIEDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNG
                     DAVLLYIMPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVISEGVETKGEME
                     TLINLGVDYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            12460..14526
                     /locus_tag="KPH29_002657"
     CDS             12460..14526
                     /locus_tag="KPH29_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBG1468242.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVYTANRLIDKNKNRYEKVIKCSQGDGGSVTYISPHDSEEEAL
                     YIAKEIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     misc_binding    15118..15328
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15554..16369
                     /locus_tag="KPH29_002658"
     CDS             15554..16369
                     /locus_tag="KPH29_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG1468243.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     gene            16397..17074
                     /locus_tag="KPH29_002659"
     CDS             16397..17074
                     /locus_tag="KPH29_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG1468244.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            17133..17855
                     /locus_tag="KPH29_002660"
     CDS             17133..17855
                     /locus_tag="KPH29_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBG1468245.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
BASE COUNT         7169 a         1928 c         3241 g         5877 t
ORIGIN      
        1 tgaataaaaa acgattgcgt caaaaataaa aaacaattaa atgtaagaat aattatgtca
       61 aaaatataaa ataaattaaa agaaaaagat tatactgaag gaaaaagatt tgaaataggg
      121 gtgaacttta catattatag ttcaccctta tacaaaaata taaaatataa tttattatag
      181 gaaaatttaa aaaaatcagt ctatttaatt tatactgatg tattgtagga ttttacaaac
      241 aaactgttat ataacttttt ttatctccaa ttttgatttt aagatatcta agaatttata
      301 acaatcttct ggatatattt ctcccatatt tgcaacttgg aatatgttag catctaggaa
      361 tttgcctttt cctggataga caacatagcc actttccctt tctaggccta atataaactc
      421 atttacattg atttctttag gtagaaatac agatgttact gtattagaca tatacttatc
      481 tggaagtaaa aatgttagcc ccaattcttc catgccattc cttaaaatag cagaacattc
      541 tttgtatctt tctattttac tgtcatcttc caaaaactca gttaaagctt cattaagggc
      601 aaatattaaa gtaacattag gtgtatttgg tgtttgagaa gatgatttag caactttata
      661 gtgtttataa agattaagat atacattctt acattgattt tctttaatat tttgtaaaat
      721 actctcttta gcacaaaggt atgcagaacc tggaaatgct ccaagagctt taccaccaac
      781 acctgttatt atatctatat taaactcttc tacatcaata tattcacctg ctgaggcact
      841 tattgcatct acaaaaaata ttttgttata cttcttagca agttttccta gttcagggac
      901 tggatttatc attcctgaac tagtttcatg gaacaccata gcaattactt ttatatcaga
      961 attttcaatt atatattttt ctaaaatgtc caggtctggg tattctgccc attcaaaatt
     1021 aggcttatat gtttttacat tataagtaaa taatatttct tctagccttt ctccaaaaac
     1081 accattacta actagcagaa ctgcatcttc tttgtcaaag atagaagata gtacacattc
     1141 atttacagat gttccagagc cagatacaat taatgagtag taattagaag ttgcattata
     1201 tagtttattg attttttgtt gtgtatcttt gaataaatcc ataaactcgt ttcctctatg
     1261 gcatatatca tagtgaagaa gtgaatttct gactttatct gtaaccatta ctggtccagg
     1321 actgaataga tatgtttttt tcattgtaaa gcctccttaa aaagttaaat ttaaattttc
     1381 gaatgactga ctatgtgtaa atattaaaat attgtgtgtt attttaacta gattatagaa
     1441 taaaataatg aatattgcaa taatatttca ataaaaatac ttttttaatc accataatta
     1501 aaaataacaa aaaatattgt aatgaatatt gcataaatcc acatgatgat ataaaatata
     1561 aataacttga atagattata tattcaaaaa ataaaaaaga atttattaat tctgaaaata
     1621 aagggggaca ttttaagtga aatatgtatt agcaattgac caaggaacaa caggaacaag
     1681 agcagtattg ataaacgaaa aatcagagtt tgtttttagc gattatatgg aacatactca
     1741 aatatatcca caatctggat atgttgaaca tgaccctata gaaatttgga ataatacaaa
     1801 aactgtgagt aataatgtat tacaacaggc ttttgaaagt ggaatcaatg aaagtgatat
     1861 aaaaggaatt ggtatagata atcaaggtga aacagtaatg ttatgggata aaaatacagg
     1921 taaaccactt tacaatgcta tcgtatggca gtgtaggaga acttatgatt atgtggaaaa
     1981 tttaaagaat ataccaggtt tagaaaataa gataatagat aagactgggt taattataga
     2041 cccatatttc tcaggtacaa aaattaaatg ggtaatagat aatgtaaaag gtgtaaaaga
     2101 aaaaattaaa aatggagaag tacttgcagg aacattggat agttggttaa tatggaaaat
     2161 gacaggagga aaatcttttt taactgatgt atctacagca gcaagaacaa tgctttttaa
     2221 tataagagat atggattggg atagagagtt gcttgacatt ataggagtgc ctagaagtat
     2281 tttagcagat ataatgccta ctagtggtga ttttggaact acagataagg aggcattttg
     2341 tggggtttct ataaaaatta ctgcttcaat ggtagaccaa ccagcagctt tatttggcaa
     2401 tggatgtttt gaaccaggaa tggttaaaaa tacttatggt acaggatgtt tcttatatat
     2461 gaatataggt gaggagttaa atatagcagg taatggtatt ttgacaacag ttgcttggaa
     2521 gataggagat aaagttacat atgcttatga tggtggagtg tatatagcag gtgctgcaat
     2581 acaatggctt agagatggaa ttggagttat aaaaaattat tcagaaactg atgacatggc
     2641 aaattccata tcaagtactg gaggggtata ttttgtacca gcatttgcag gtatagcagc
     2701 accttattgg gaccaatatg ctagaggcac aatggttggg ataacaggaa gtactaaaaa
     2761 agaacatata gtaagggcta ctcttgagtc tgttgcactt caagtcaagg atgttgttga
     2821 ttccatgaat ttggcttcag ggaaagaaat aaaaatgttg agagtagatg gtggaattac
     2881 taaaaataac tttacaatgc aatttcaatc tgacatatta gatataccaa ttgaaattgc
     2941 agaaaatcct gaaacgacag tacttggtgt agggtatatg gcagggcttt cctgtggtgt
     3001 ttggaacagt attgatgaaa ttagaagtga atttacaata agcaaaaaat atattccaaa
     3061 tatgactaag caaaaaagag aagaaatatt acttggatgg aatcaagcag taaaaagagc
     3121 tatgaattgg gaaaacgata ccaaaaaggt gtgtgctaat taagttacaa tagagtattt
     3181 tacatgtata ggaggaatta aaaatgccct ttgataaaat tatattatac ataatggctg
     3241 tgggagtgct tttaggagcg ttagatagaa taattggaaa taaatttgga cttggagagc
     3301 agtttgaaga agggtttaat tcaatgggtc cattggcttt aggaatggtt ggtatagttt
     3361 gtttagctcc agtaatttca gatgtactag gtcctataat aataccaata ttcaatctct
     3421 gtggagctga cccatctatg tttgcaacta ttttagcgaa tgatatggga gggtatccat
     3481 tggctatgga acttgcacac aataaagagg caggaatgct tgcaggatta gtcgtgtcat
     3541 ccatgcttgg atgtacatta gtattttcaa ttccagtagg actaggtctt atagaatatg
     3601 aagacagacc attctttgca aaagggttac tgataggctt gataacaata ccttttggag
     3661 gtattatagg aggattaata gcaggattta acttatcaat ggtaattata aatatgatac
     3721 cagtgataat attggcagta atattggcag taggtttaaa atttaattct acacttatga
     3781 taaatgggtc attagttttt ggaaaattta tatctatatt gataacgata ggtttgggag
     3841 cagcagcatt tcaagaaata acaggtatag tacttataaa aggcatgaca cctataagag
     3901 aaggtattca agtaattgga agtattgcaa tagtgttatt aggtactttt ccaatattac
     3961 atttattagt taaagttctt aataaaccac ttacaatagt gggtggaaaa ttaggtatgg
     4021 atgccactag tgcagcagga ttagttttta ctttagccaa tagtattcca gtatataaaa
     4081 tgatgaaaga tatgagtcca aaggggaaag tagtaaatac tgcttggctg gtttgtgcta
     4141 cagcagcttt gggggaccat ttaggattta cagcaggagt agttccagag atgattacac
     4201 ctgttgtaat tggaaaattg gttggaggag tattggcaat tgcacttgct atacttatga
     4261 caaaagattt aactttggaa gaagaacaaa gtattaaaat gaagaaagat gcagtgtaga
     4321 ataaacaata cttaagtcaa atttaagtta ttttaaagta ggagtttaaa taactaaaaa
     4381 taaattaaaa agacagacaa ttaaaaaata atcgaataac caaaactgat aaattaaata
     4441 aaatggcaat caaataacta aaaatgataa gctaactgaa agtagttaca taattaaaat
     4501 ataaagtata gtttagacat aagtggagct aaataaataa ttatttggct ccttaatttt
     4561 aatttattac atcaaggaac taaaaatcaa tcatacaagc taattatcag ctatgactaa
     4621 aggtattcct ttttgagaaa actctattat tatattatgg tctatactag cagaggtaat
     4681 aatagtatct atgtgttcta taggagctac tgatgcaaat gaatttatac caaacttagg
     4741 aggttcgaca actactatag attttctaga cctttcaatc attttttttc taaccagtgc
     4801 ttcttctaag tgatagtcag agattccatt ttcaagagtg atacctccag cacctaaaat
     4861 agctttatca acattaaatt gttcaatagc tgagagtgta agtggaccag aaaaagaacc
     4921 ttcagtttct ctaagatagc caccaagcac atatactttt atattagtat tcataagttc
     4981 atttgcaact aaaattgagt tggttattac agttagattc tttttatctt tcaaattttt
     5041 tgaaacttca agtacagtag ttccagtatc cataaaaata gtatcatttg tatttataaa
     5101 ctcactacat ttaatagcaa tctgtttttt atcgtttgag tttatttttt ttcttttaac
     5161 atagttaagt tctacgctac ttgctataac tggtatagca ccaccataaa ctctttttaa
     5221 aagaccgata ctttccatat gggctaaatc tcttcgtgca gtttctattg atatatcaaa
     5281 catttgaact atgtcattta ttttaattat attatctgat ttgagaatac ttaaaatctt
     5341 ctcgtatctt tcctgtggta acataaaata tcctccttgg actagtatta ttttagtttt
     5401 ggcataactt acaattaagt cagagtttag gtatatttgt taatgtatgt atatttatca
     5461 aataatatta taagtgttat gcaagataat tttaccatat ttaaaactta catttaagca
     5521 aaaaagtaaa aatagatgtt taataattca aaataactca aattttataa aacaattgac
     5581 taaaatatga ttttattata taatttacta gtatagagat atggagaagt actcaagtgg
     5641 ctcaagagga tcccctgcta agggattagg ctgggtaacc ggtgcgaggg ttcgaatccc
     5701 tccttctccg ccaattgtta aaggctattt ttattaaaga tagtcttttt ataatgtcat
     5761 aaatgattag atatgtataa ttaaagaaag gagggaatag atgacaactt taattttttt
     5821 catagtgggg atagcatctt tggtagttgc ttttgttatt gatgggggac atattggagt
     5881 attatttgta ttttcatcag cacttgttgt gtttggtgga actataggcg ctgttggagc
     5941 ttcgactcca ttccctgtgt ttaaaaagtc tataaagata atgttattag cttttaaaaa
     6001 taaaaagcct aataacatag aaaatataat ctatttcaaa gaaattgcaa caaaggctag
     6061 gaaagagggg cttcttagtg ttgacaaaga aataagtgag aatgatgaat tagacccttt
     6121 tattagaaaa gggcttgaac ttatggtaga tggggtggat gcagttacta taagaaatat
     6181 attggaatca cagatacaga taacatcaaa acgacataaa gaaggaatag caatttttga
     6241 ttcagcaggt ggatatgcac ctacactggg gataataggt acagttatgg gattagtaca
     6301 tattttaggt aatctttcaa gtaatccaga tgcattagga cctcaaattt ctgttgcatt
     6361 tttggctact ttgtatggtg ttggttctgc caatttaata tggttaccaa tagcatcaaa
     6421 acttaaggct ataaatcaac aggaaataag tgaaagactt ttggtgatag aagcaatatt
     6481 atccatactt gagggtgata attctaattt aatgacagaa aaattaaaaa tatttttaaa
     6541 ttcaaaagaa ctaattgaac ttgaagaaat tggtggtaga gctgaataat ataaaaggat
     6601 ggaatgttta tgttgtatga tgaagatgaa aaagaagaaa acaatgagcg atggctttta
     6661 acttattcag acctcataac attacttatg attttctttg taataatgta ttctatgagt
     6721 aatgttgatg cggagaaata taaacaatta tctcaatctc taaattctgc ttttggtggt
     6781 tcttctggag ttattgaagg tggtaatagc aaaatagaac cagtagttga accaggaagt
     6841 aatgatttag atagtttaca aaatgctaag tttaaaaaag ttggtgaaga aatccagaag
     6901 tatttaaatg aaaatggtat ggctaattca gtatcattaa gagttcaaga tagaggatta
     6961 gtaataagcc ttaaagatac aatactattt gatacaggaa aagcagttgt aaaggataat
     7021 tctagagata agattattca aataggtaag atgttaaatg aaatgaatag ttacatgaga
     7081 gtagaaggtc atacagacaa tatgtctata aaaaattcag agtttaagtc aaattgggat
     7141 ttatcagtta tgagagcaac caatgttgtt caacttctta ttgacaatgc aggtatagca
     7201 ccagataaat tatctgctgt aggatatggt gagtttagac caatagcaga aaattcatct
     7261 gaagaaggta ggtcaaaaaa tagaagagtt gatatagttt tagttgatag taaatatgat
     7321 aatgtagaaa atgtttcaaa ggataaatag ttaaattagc tgtctcaata cagaattaat
     7381 tctaaaggag atggcttttt tatgcgaaaa ttaaaaatag atacatagta agttaccttt
     7441 ttgtaggaat atatggtatc ttaaatatat aatatttaaa taatatataa gctcaattaa
     7501 aaaaatttta tagaggatgg gaaataaatg gggaataaaa ataaaaatac gaaatataaa
     7561 gataaaacaa gagaaagata taataaaaaa gttgagaatt taaataaaga aaaattaaat
     7621 aaagataata aacagtctaa gaaggtagat aagattgatg atattattaa taaatctagt
     7681 attgtattat gtgtacttct agtggtgttt tcattacttt acttatttaa attggttcaa
     7741 cttaatataa taatgatact tttagtaatt acagtagcta ttcaacaagg attggtgtca
     7801 tttggtgcat ttagaaataa aaattatgta aaagctttat tttctatatt tgtaatagta
     7861 tgtttcttaa ttgcttttaa atatgttgta aatcctgttt aaatatatca ataaacgttc
     7921 tataattaat ataaatagtg aagttacagt tttaatgcta ttataaattt tataatttga
     7981 aggagtagat tttatgaata ggtgtaagtt tgcaaaatta attatcacat caatgattgt
     8041 tttattactt acaggatgta gtttaaaaaa ggaagataat tataagttag acccagaaaa
     8101 tccgacatca atacaaattt ggcattatta taatggacag caaaaaatgg cttttgataa
     8161 gcttgttaag gaatttaatg acactatagg tgctgaaaaa ggaattattg ttgaagcgtt
     8221 tagtcaaggt aatgtagttg atttatcaga gaaagtttta gattctgcaa ataaaaaagt
     8281 tggctctgaa gatgtgccaa atatatttgc agcatactca gatactgcat atgaaatgga
     8341 taaattaggg ttactagtag atatggataa gtacataagt aaagaagaat taaatgaata
     8401 catagacgaa tatgtagaag aaggcagaat aaattctaag gatgagttta aacttttccc
     8461 aattgcaaaa tcaactgaaa ttttgatgtt aaataaaact gattgggata aatttgcaaa
     8521 agctacaaat tcaaatataa atgatataaa aacaattgaa ggattagtag agacatcaaa
     8581 aaaatattat gaatggacag atagtttaac gaaagagcct aatgatggta aagcattttt
     8641 tgggagagat gctttagcaa attatattat tataggaagt aaacaactag gagtagaaat
     8701 tttttctgtt aaagatggta aagttacact aaatattgaa gaaaaagtta tgaaaaagct
     8761 atgggataat ttctatgtac catatataaa tggatatttt gcttcttatg gtaagtttcg
     8821 ttctgatgat gcaaaaacag gtgatatatt agctcttgta ggctctagtt caggagcagc
     8881 atactttcca gatagagtaa tgctaaatga taatgagagt tataatattg aaacatctgt
     8941 ttttcaagca ccacgtttta aaggagaagg aaaatatgct gtacaacaag gtgcaggaat
     9001 ggctgtaaca aaatctacta aagaagaaga atatgcatca gtagaatttt taaaatggtt
     9061 tacagataaa gagcctaata taaaattctc tattgcttct ggatatttac cagtaaagaa
     9121 aaaaacgaat gatttgaatg ttataaaaga agttatgaag aaagataatt tgagtttatc
     9181 tgataaagtt gaaggttcaa tagcctcatc tattgaacaa attaacagtt gtaatttata
     9241 cactaataaa gcatttgatg gtggaactaa agctagacaa atacttgaat attcaatgct
     9301 tgataaagct aaagaagatt caaataaaat aaatgaattg gtaaaaagtg gaacttctaa
     9361 gaaggatgcg gtagcacaat ttaataatga taagaatttt gaaacttggt ttgaaagttt
     9421 taaggctgag cttgaaaatg ctataaaaaa atagatgaat tggcagttag tgtgtagaca
     9481 agcacaaagg tataggtgga ataagtatgg aaaacaatct aagaaaaaac aattcaataa
     9541 tgaaaaagat gatatttccc ctaattattg ttatgctaat tcaaacaagt ttattttgtg
     9601 ccacaatttt atggggagga actattaaga aattaaatga taattcgttt gacatcttaa
     9661 atgaaagagt aattaatcgt aaaaattata tacaaaatga aatgttacaa agatggtcaa
     9721 atacaagtga aacggaagct acaattaatt cctctgtaaa aaaaatatta aatgaaaaaa
     9781 atgccttaat tgaggatgta ggcaacaatg aagaaattaa caaagatatt ataaaaagca
     9841 tatcaaatga tttaatatat cttttgagaa aaaactctgt tacaggagct tttataattc
     9901 taaatgacaa aaatattaac gataagccaa tagatagtaa aggtgaaata agtaggactg
     9961 gattgtatat aagggattta gaccctaagt ttaatccaaa tgataattct gatttgttaa
    10021 ttgagagagg ttcatcagat atagcaagaa gttttggaat atctatggac agttattggg
    10081 aacctaaatt ttcatttaaa agtgaaaatg agcaagaggg agatgatttt ttttataaac
    10141 catttagagc tgcaattgat aatccaaacg tatcaagttc taatttggga tactggggaa
    10201 gtaagttttt attgacaaat gcaggtgata ggcttatgac atatacagtt ccccttattt
    10261 atgaagatgg aactgtatat ggagtacttg gaatagattt aagtgtaaat tatttaagag
    10321 aattacttcc atactcagag ataaatggaa ataaaaaagg taattatatt ttaggtgtag
    10381 atagaaataa tgatatggcc tttgaaaatg taatttcaaa tggatactca ttaaaagaaa
    10441 aattttggga tggtttacat tctaatttag agcagaagga atcgtataaa aatatatata
    10501 agattaaaaa taaagataat gaatgtggta atggcaacat ttatgctagt gtacaatact
    10561 tagatttata taactccaat actccatttg aaaatgaacg ttgggctctt ttgggtatta
    10621 ttgaagaaaa aagtcttttg tcattttcaa aaaggataga gaaattagtt ataatatcta
    10681 tagcaatttc tttattaata ggtctaatag gtatttacat tgcatcaaga ttatttacaa
    10741 aaccaattgt tcttttagcc aacaaagtta gaaacagtaa tccatcaaaa ccagtaaatc
    10801 ttgataagat aaatatatct gaaatagatg aattatcttc agctatagag ttactcagtg
    10861 caaatgttgc agattcttca tcaaaattat cacagattat tgaaatggta gatatgccaa
    10921 tcggggcttt tgagttatac aaaaacgcag agaatgtttt ttgtacaaaa gataaaaaac
    10981 ttaagtggat aagaatgaat atctttgaag atgagctaaa ggttcttggt gttgttgttg
    11041 atgtcacaaa agaatttatt gagaaaagga agatagaata tgaaagagat tttgatattt
    11101 taacgaatct tttaaatagg agagcttttc attcacagat acaagaaaaa tttaagaata
    11161 aagatgaact taagatagca gcatttatta tgtgggattt agataatcta aaatatatta
    11221 atgatacata tggacatgat gctggtgatg aatatattag gtgtgctgca gacatactta
    11281 agaagtttat atcatggaat ggtatagtat ctagactttc aggtgatgag ttttatgtat
    11341 ttatatatgg atatgaaagt aaagatagta taagagatat tattaattct gttaagaaac
    11401 agatgaataa taccttatta aaacttcatg atggaacaga atttaaaata agggcatcag
    11461 caggaatttc ctggtaccct gatgattcaa ccaactatga tgaattaatg agatacgcag
    11521 attttgctat gtatcaaatt aaaaatacaa tcaaggggga agttagtgaa tttgatatta
    11581 atatatataa taaggattca ttcttattga gtaatagaga agaattaaac aagttaattg
    11641 atggtcaatt agttgagtat gcatttcagc ctattgtaga ttgtaaaggt aaagtatttg
    11701 catatgaggc acttatgaga cctaaaataa aaacttttag ttcaccaata gatgtaatta
    11761 gattagctcg ttcacagtct aaattatatc aaattgagcg tattacatgg tttaaagcac
    11821 ttaaatcatt taaaaaatac aaagaaaaat ttggcgattg caaactgttc ttaaattcta
    11881 ttcctaataa taatttatca aaagtagatt ttgaggaaat tgaagattta tatggtgaat
    11941 atttaaatag agtagtatta gaaataacag agaatgataa gattaatgaa gattttattg
    12001 agaaaaagaa agcatatata gaccattgga atatagaaat tgctttagat gactttggga
    12061 caggttacaa cggagatgct gtactattat atataatgcc agactatgta aagattgata
    12121 tgtctattgt aagaggtatt gataaagatg aaaacagaca gaaaatatta aaaaatctta
    12181 tctcatattc taacgaaaga aatattaaag tgatatcaga aggtgtagaa acaaaaggtg
    12241 aaatggaaac actgattaat cttggagtag attatatgca gggttactat attagtaaac
    12301 catcatttat acctgaagat ataagtgaca aaattaaaaa agagctcaag atatgtcatg
    12361 aaaataggaa tgatgtggtt taatagagga aaaattaaat ttatttttat gaacttttag
    12421 tttataatta aaaagaaaat aatataatta taaattaagg tggtggagct aataatcaca
    12481 acaaacatag atataagaaa gttaaatgaa aatcaattag aagcagttga acatatagat
    12541 gggccttgca tgatacttgc agggcctggt tcaggtaaga ccagagtaat tacatataga
    12601 atagccaata tggttgtaaa caaaaatata gcaccaacaa gaatcttggc aataagtttt
    12661 accaaggcat cttcaataga aatgaaaaat agagctttga atttaagtga tgatataaga
    12721 cttaataagg taacctatgg tacttttcac tctgtatttt ttaaaattct aagatacttt
    12781 gaaagataca atttagacag tatatttgat gaaaagtcaa agcgaatgac aatcaaggca
    12841 atcctaaaaa gtttaaatat agagaatgct gatgatgatg agaatatagg acaagttata
    12901 aatgaaatct cctatgtaaa aaatgaactt atggataaaa atgaatttaa ttcagaagta
    12961 ttaacaaaag atgagttttt aaaagtatac aacttatacg aagaacaaaa atctaaagtt
    13021 aacaaaatag attttgatga tatgcttata agaacttatt atttactttt gaacaataag
    13081 tctgcactag aaatggttag aaatgtgtat aaatatatat tgatagatga atttcaagat
    13141 ataaataaag tacagtttga agttttaaag ttaatttgta gcccattaaa caatatattt
    13201 gcagttggag atgaagacca aagtatatat ggatttagag gtgctagacc agattttttg
    13261 cttgaatttg aacaatactt taataatact aaaaaaataa tattagatat aaattatagg
    13321 tctaaaagtg agatagtgta tactgcaaat agacttatcg acaaaaataa aaatagatat
    13381 gaaaaagtca ttaagtgtag tcagggggat ggaggaagtg ttacctacat ttctccacat
    13441 gattcagagg aagaagcctt atatattgcc aaggaaataa ttgacgaaat acaaaaagat
    13501 tatgtagagt attcagattt tgcagtaatt tatagaacaa atatacagtc aagagcatta
    13561 gtagatgtat ttatggatat gaggattcca tttgtagtaa aagactcagt aattacaata
    13621 tatgaccatt gggcaagtca agatatacta gcttatttaa gaataggtat taatccaaaa
    13681 tcaaataaag attggttgag aattataaac aagcctttta gatatatatc caaagacagt
    13741 ataaatatgg ttaaggatga aaaagacttt ataacagcat taataaataa gtgtaatctt
    13801 catcctaaac aagttaaaac aataaatgat ttggaaatag atttaagtta tttaaatact
    13861 ttaaatccta aaaatgcaat atcttatatt agaactagtc ttgattatga tagatacgtt
    13921 ttagattatt gtagcaacag aaagataaaa actaatggac ttgtagagat attaaatgaa
    13981 cttgagagtt cagcaactaa ttttaatact gttacagagt ttttagagca tattgataga
    14041 gtaaagactg agttaacgga aaataacaag aataagcaga ctgagggtgt catatttaca
    14101 actatgcata gtgcaaaagg attagaattt agaaatgttt atattatagg tgtaaatgaa
    14161 ggaacaatac ctcatgaaaa atcatatgat atttgtaagg aagagaaaaa agaagaacaa
    14221 ttagaagaag aaagaagact tatgtatgtt gctattacaa gagctgaaga aaagttatgt
    14281 ataagctcta ctttaaataa gtatggtaag aaagttgata aatcattatt tattaatgat
    14341 ataaaatctc caacgaaaaa agaaattgac agtattggtg taggggataa ggtataccat
    14401 aaaaaatttc atgaaggaga aataattaaa aaagatggaa ttatgtttac aataaagttt
    14461 aaagatagag agcgaatctt ggatttgaaa acttgtttat tgaagaatat aatttatact
    14521 atttaattta gaatctaaat ttagatagaa atataatgta ttatttatta tgtgcaagac
    14581 aaatagatat gtgtaaaatg tattatttaa cttattagaa aaagaaataa ggatttaata
    14641 tattatttat agttgtttag aaaaagcaac aatgtggaaa atattattta gtattattaa
    14701 agaaaagcaa caatgtgaaa gatattattt agtgtaattt aagaaaaagc aacaatgtga
    14761 aagatattat ttagtattat taaagaaaga gcaacagtgt gaaagatact atttagtatt
    14821 attaaagaaa aagcaacagt gtgaaagata ttatttagtg taatttaaga aaagcaacaa
    14881 tgtgaaagat attatttagt attgtttaag aaaaagcaac aatgtgaaag atattattta
    14941 gtattattta agaaatagct aaaaatatga aatatactat ttataattac aaaaataata
    15001 tcaataaata taaacagtag tatttagata atttacaagt aaaaattatt aaaaaaataa
    15061 aaaacttatt gacaaaaata ttataaggaa gtacaattgt attaacaatt taaggaaaca
    15121 aatccaatga tagagaaagt agcatatatg gatttacagc gagttagggg ttggtgtaag
    15181 cctagcaatg aagtctatgt gaagagagct ctggaggaga ttatcagaaa ctttagtatg
    15241 ataattggtt tacccgcctt aagggattga gaggataagt acttacttat caaaaagagt
    15301 ggtaacgcgg atataattcg tctcttagct gtaaagctaa gggacttttt tgatttaaaa
    15361 ataaaaatat ccatacagct taaatgctaa cagtcacaaa ttttgaaagt aatataagta
    15421 tttatcaata agagtggtaa cgcggtaaag tcgtctctta gtcattaaag actgagagac
    15481 tttttttgta actcgaaaca tttaagtatt aaaatctgag ggaaattata attaaaagag
    15541 aggggtagtt aaaatgaaag tatttaaaaa attattaagt ttaggattag tattaggatt
    15601 gacattatcg ctagtaggat gtagtggtgg aggagaaaaa acaaagttag aacaaataaa
    15661 agacaatgga aaattagttg ttggtactag tgcagaattt cctccatttg aatttcataa
    15721 ggtagtagat ggaaaagatt caataaaagg attcgatata atgttggctg aagaatttgc
    15781 aaaagagctt ggtgtaaagg ttgaaattaa ggatatgtca tttgatggat taataggtgc
    15841 tttaaatgca gaccaagttg atattgttct tgcaggtatg tctccaacac cagagagaga
    15901 aaagagtgtt gatttttcag aattatatta tttaagtaga aatgcagtta tagttaaaga
    15961 tgcagatata gacaaagtaa aaactgaaga tgatttaaag aaacttagag ttggagttca
    16021 agcaggaagt atccaagaag aatatgtagt taacacttta aaaatgacaa ctacaaaatc
    16081 tttaaaagca atacctgatt taattacaga attaaaaaat ggaaacatag atgcagttgt
    16141 tactaatgaa gcagtttctt taataaatgt taaaaaatat gatggaataa aaatggcaaa
    16201 tactgaagtt ggtaaagatg taacagaagg tatggcagca gcaatcaaaa aatctgacaa
    16261 taacaaagat tttatagaat tattaaacaa aaagataaaa gaattacaag atggtaagaa
    16321 gatagaagaa tttttaaatg aagcatctac tgaagctgct tcaaattagt aagaaagtta
    16381 tgtgggagat gaaattatga gtttagattt tagtttttta agtagatttg ggacttcttt
    16441 tttggaagga acaggtgtga cagtatcaat ttctcttgtg gcattatgct ttggatttat
    16501 aataggtata attatctgta tggcaaaaat atcaaagagc aaagtattaa gagcaatatc
    16561 atcaatctat attgaggttt taagagggac accattatta gtacagatat atatagtatg
    16621 gtttggttta cctcaattag gaataagatt ccctatgttg tttggtatac caagtgaatt
    16681 tatagctagt gcatttgcat tatcagtaaa ctctggtgca tacgtggcag agatacttag
    16741 atcaggtata caatcagttg ataatggaca aatggaagca agtagatcat taggattaaa
    16801 ttattggtca actatgagat atataataat acctcaagct ataaaaaata tattaccttc
    16861 attagcaaat gagtttatta cacttgtaaa agaatcttca ataatatctg taataggagt
    16921 tgttgagata atgcgtactg ctgatatagt aaaaaatgca gcatttagag cattagagcc
    16981 attaatagta gcagcagcaa tttactttgt tataacattt actctatcaa gattagttgg
    17041 attattagaa aagaaactat ctgtgtctaa ctagatgaga gcatgtttag catttaggga
    17101 aaacatagaa tagtaaaaag gaaagtgata atatgattac aataaaaaat ttaagtaaat
    17161 cttttggaga tttaaatgta ttaaaaaata tagacttaga aatcgctaaa ggcgaaataa
    17221 tggttatagt aggtccaagt ggttctggta aaagtacact tcttagatgt atgaatttac
    17281 tagaaatacc tacaggtgga gaaataatat ttgaaggaaa aaatttggtc gataaaaaaa
    17341 ctaatattga tgaggttaga caaaatatag gtatggtgtt tcaaaacttt aatttattcc
    17401 cacataagac tattttagat aatataacat tagcaccaat taaattaaaa aagatgacta
    17461 aggaagaagc agaaaaaaaa gcagaaattt tgttgagtag agttggtctt ttagataaaa
    17521 aagattcata tccatcacaa ttatctggag gacaaaagca aagaatagca atagctagag
    17581 cacttgctat ggaaccagat atgatgttgt ttgatgagcc aacatctgct cttgacccag
    17641 aaatggtaaa cgaagttttg gatgttataa aagaacttgc taaagaaggt atgacaatgg
    17701 caattgtaac tcatgagatg ggatttgcta aggaagttgc agatagagtt atttttatag
    17761 atggaggaag tatacttgaa gataatactc cagaagaagt tttcggaaat ccaaaacatg
    17821 aaagaacaaa agcattttta gcaaaagtgc tataatatat aaaaaggatg ttattgatat
    17881 tagaaattca atacatcctt ttttagtgat ggttaatatt aattttaaaa ttaaagttat
    17941 attaatatta actttatttt tacgattttt tgcagaaaaa aaaagagctc ggggtaagct
    18001 ctttttttcc accgaattaa tttccgttac taatccgatt tttaataaat ataaaatata
    18061 tccttatgaa agggtattgg gaatagatat attaactaac tagggttacc agggggataa
    18121 ccttaattta ttataacaaa atgcgacaca aaaatcaata ttttttttga aataaattat
    18181 aaaagttatt tttataacaa gtaagcttat aaatt
//
LOCUS       DAELXX010000084        17672 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.084, whole genome shotgun sequence.
ACCESSION   DAELXX010000084 DAELXX010000000
VERSION     DAELXX010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17672
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.084"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            228..1157
                     /gene="glsA"
                     /locus_tag="KPH29_002661"
     CDS             228..1157
                     /gene="glsA"
                     /locus_tag="KPH29_002661"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBG1468246.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            1255..1818
                     /locus_tag="KPH29_002662"
     CDS             1255..1818
                     /locus_tag="KPH29_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1468247.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            2306..3142
                     /locus_tag="KPH29_002663"
     CDS             2306..3142
                     /locus_tag="KPH29_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBG1468248.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHIGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKRELKMIKEILS
                     SK"
     gene            3394..4389
                     /locus_tag="KPH29_002664"
     CDS             3394..4389
                     /locus_tag="KPH29_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBG1468249.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRNKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKDYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(4646..5050)
                     /locus_tag="KPH29_002665"
     CDS             complement(4646..5050)
                     /locus_tag="KPH29_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBG1468250.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     FLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            6050..6964
                     /gene="queG"
                     /locus_tag="KPH29_002666"
     CDS             6050..6964
                     /gene="queG"
                     /locus_tag="KPH29_002666"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBG1468251.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKDRQQKGYLTG
                     MEEPIIENRINPRHIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFVDNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEMPTTHILEFKENIIDYLDYDEIQNISNKEFKR
                     RYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            7070..9307
                     /locus_tag="KPH29_002667"
     CDS             7070..9307
                     /locus_tag="KPH29_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG1468252.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNRLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDKSDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNESSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTEQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
     gene            9504..10556
                     /gene="nth"
                     /locus_tag="KPH29_002668"
     CDS             9504..10556
                     /gene="nth"
                     /locus_tag="KPH29_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBG1468253.1"
                     /translation="MKNVNLKEINEKDVIVEEDINLKGMDKKDVIEKEEVNKKSIDKK
                     DIIEKEEIDKKNVKKVEVQKEIDKMISKKDEIIDEKDIENFRYIEEDKKSIKVSKKST
                     KKGETEKKTKKSSSNKATQKQSKEVKSKSKIKSESANKKDVNKILDELEKLYPDAKCE
                     LNYGTAFELLIATILSAQCTDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGL
                     YKSKSQKIKDTSEQLCELYDGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDT
                     HVFRVSNRIGIVDEPNPQKTEFALMEAIPKERWSHSHHVLIFHGRRMCKARNPECASC
                     PIKEDCNYYKELNETK"
     gene            10750..11220
                     /gene="trmL"
                     /locus_tag="KPH29_002669"
     CDS             10750..11220
                     /gene="trmL"
                     /locus_tag="KPH29_002669"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBG1468254.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            11256..12104
                     /locus_tag="KPH29_002670"
     CDS             11256..12104
                     /locus_tag="KPH29_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBG1468255.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKRLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVDEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
     gene            12477..13481
                     /locus_tag="KPH29_002671"
     CDS             12477..13481
                     /locus_tag="KPH29_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG1468256.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNAALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGIKSSSRDKFESGFREGVKFSNSSIKDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSI
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            13606..14718
                     /locus_tag="KPH29_002672"
     CDS             13606..14718
                     /locus_tag="KPH29_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG1468257.1"
                     /translation="MKIKRILMACFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAKS
                     KQSESKSVPTSAEKDKLNSKSKNEEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDLIIAVGARFAEPIKEAATKYP
                     KQQFAIVDYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIKNVAKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
     gene            15141..15902
                     /locus_tag="KPH29_002673"
     CDS             15141..15902
                     /locus_tag="KPH29_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468258.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            15905..16723
                     /locus_tag="KPH29_002674"
     CDS             15905..16723
                     /locus_tag="KPH29_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HBG1468259.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKAEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            16903..17505
                     /locus_tag="KPH29_002675"
     CDS             16903..17505
                     /locus_tag="KPH29_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HBG1468260.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
BASE COUNT         7296 a         1613 c         3253 g         5510 t
ORIGIN      
        1 gttaggttag aaatttttga cgttataaga atttaaatta ctggattatt agattactaa
       61 attactagat tattaaatta ttaagctatt taaactgttt aaattgtttg aaaaatgaat
      121 tgattcttgt agaaaatagt tttaaagtat aatttttttt aacactgttt tattatagta
      181 caaacttgtg ctaaaataga atatataaca taatataaga ggtgcatatg aatacgttag
      241 atgagacatt attaaaagaa attataagtt ctaataagaa gtatactaat tatgggcaag
      301 tcgctagtta cataccagaa ttaaaaaacg ctagaagaaa tgatttagga atttgtatca
      361 tagatagcga aaataatctt tatagtgctg gaaactgtag cacaaagttt actatacaaa
      421 gtatttcaaa accaattgtg cttgctatgg cacttatgga taatgattgg gaagacgttt
      481 tttcaaacgt tggaatggag ccaagtgggg acccatttaa ttcaataatg aaacttgaaa
      541 ttaatgacac aaaaaaaccc tgtaatccaa tgataaatgc aggggctata gtaactactt
      601 cccttataaa tgggagttgt ttagaagaaa aagaagaaag aatgcttagt ttctttagaa
      661 agttagcaaa aaatgataat ataggaataa actatgatgt ctataagtct gaaaaaatga
      721 ctggagatag aaacagagct atggcttact tattgaaaag tgatggattt atcagaggga
      781 atgtagaaga tgttctagac ctttatttta aacaatgctc aattgagata gattctgtag
      841 atttagcgag aattggaata aatcttgcaa attatggagt agatatagaa aatggtgagc
      901 atttaatgag tgaaatggtt tctaggattg tcaagacatt tatgatgact tgtggaatgt
      961 atgatgcttc tggggaattt gctattaaag ttggcattcc tgcaaaatct ggagttgggg
     1021 gtggtataat ggcttctgtg ccgggacgca tggggattgg tgtttatgga cctgcgcttg
     1081 acaaaaaagg taacagcgtc gcaggagtta aagttttaga ggagttgtca aataaactca
     1141 aattaaatat tttttaaaat attaagaaag gatttttttg acaacaacat ataatttaat
     1201 atataattaa attgtaaaga aatatgaatt aaataaattt tttaggaggg gtcaatgaaa
     1261 gaggtattgg tattacgaga tttggagtgc ataaaggcta ttgcgcatcc acgtagaata
     1321 gatattctaa aagcatttga caaatcgcca ttgtcagcaa aacaattatc tcaattattg
     1381 gaagaacctc atgcaaaaat aaactaccat ataaaaatgt tatacaaagt tggtatttta
     1441 gaattagttg aggaaaaaat aaagtcgggg attgtggaaa agtactatta tccaagtgca
     1501 aaaaatgtgg ttatagggaa taggatactt aatttttctt tagacaatgg ggaagaaaaa
     1561 gaagaattgt acatatcaaa atttgaaaat atgagtgaag ttttttataa ggcagcagag
     1621 gaagatgttt tagaaaatga aaatatagtt gattatcatg atatttctct tactcatgat
     1681 gaattagttg aacttagtga cactatgaag tcaaagatag atgaaatttt aaataaaaga
     1741 caacacaatg ttgaaggttc taagtatgat atagctatgg ttatagttcc tactcttgag
     1801 gaggaatgtc ctagttagag aattaattat ggattaatta aagatataat attagagatt
     1861 gaacatcaaa atttacttaa taatcaaaaa atagtttttg gtatgggtaa aacaatgatt
     1921 tttaatctct atttttttgt tttcttatac ctattgtatt tgtaaataat atgtatagtg
     1981 taagatgtat acactatctg agaagtgctt agataatata caaagtatgt aagattataa
     2041 tatttgaaat tgattaaaat gggtataatt aaagtgtaaa tattaaaatt aaaaattaat
     2101 ataaattaaa tcaatgctag aaatatgtta aaagaggaat gagattagtt gggatagaac
     2161 atcccttaga gcttatgaat ttttagtgaa aaagtactat caaataggaa tctccatctt
     2221 catatgttgg ctaaatatag ttaaggtgga gtagttcaaa attaaaattg agtacaagtc
     2281 gtagtacaaa taaaaggaga ttaatatgat taaattagga acacatcttt caatagcaaa
     2341 aggatttgct aatgcggctg agacagctgt atatattggt gcaaatacat ttcaattttt
     2401 tagtagaaat ccaagaggag gaaatgctaa agaatttgat gaaaaagata taattaaatt
     2461 tcaagaaatt agaaaagaaa ataattttgc accactgctt gcacatgcac catatactat
     2521 gaatctgggt ggtacaaagg atgatgtata cgaatttgca acaagggtga tagatgagga
     2581 tataagaagg atggactcat taggaattga atacatgtgt tttcatcctg gaagtcatat
     2641 aggtggtggt gtagattttg gtatagatag aatagtaaaa ggattgaaca atgccataaa
     2701 gggtgatgaa aatataacta tacttcttga aactatgtcg ggaaagggaa ctgaaatagg
     2761 ttttaaattt gagcaattaa aaagtataat agacaaaatt gagcataaag aaaaaatagg
     2821 tgtgtgtctt gatacatgtc atattttttc agccggttat gatatagtga atgatttaga
     2881 tggggttttg gaagaatttg ataaggttat aggcatagaa agattgaaaa ctgtccatct
     2941 aaatgatagt atgatgccat ttggagacaa aaaagacaga catgctccta taggggaagg
     3001 aaagatagga ttaaaagcat taattgattt tatggaacat cctagtctaa aacatttacc
     3061 atttttctta gaaactcctt ttgatgatga aggtcacaag agagagctta aaatgataaa
     3121 agaaatacta tctagtaaat aaacctttat taaaaatatg tgaaatactc cgccctcaac
     3181 ataattaagg tggagataca atcatgaaat gtgataattt aaattaatgg ataggaataa
     3241 caaaaattgt atacaaatta actgggtata aagtatgatt atattatgaa tagtatgtaa
     3301 atatattgca aagattctca taatttgttg tataattaaa ggtaacatat ttatattata
     3361 taaaaaatta ttaaaagtta ggtggggaaa gatatgagaa atttaggcaa tactaacatg
     3421 aaaataaaga gagttggctt tgggggcatt ccaatacaaa gaattactca agatgatact
     3481 aacttagtta taaatgagtt agaaaaacaa ggaataaact ttattgacag tgcaagaggg
     3541 tatacaataa gtgaggaagc aatcggaatt gccattgaag gaaaaagaaa taaatttttc
     3601 ttagctacta aaagtatgtc cagagattat gattcaatga agagagatgt tgaaataagt
     3661 ttaaataatt ttaaaactga ttttatagat ttatatcaat tccataatgt taaagaagag
     3721 gagtatgata atctttttaa agataaaatg gcttatagtg cccttttaga ggcaaaggag
     3781 caaggtaaga taaaacatat tggtataact agtcacaatt taaatactat agaaaaagct
     3841 atagaagatg gaaaatttga cactatacaa ttcccttaca atatagttga aggacaagca
     3901 gatgaagtgt ttaaaaaagc tcatgaaaaa ggaattggga taatagtaat gaaaccttta
     3961 gctggaggtg ctttagacaa tgcaacactg gctattaaat atatactatc taaagattac
     4021 atagatgtag ttataccagg tatggagagt gttgaacagg ttagacaaaa tgtcgctgta
     4081 cttgaaaatc ttgttctaga tgaaaaagat aataaggaaa ttgaagaaat aagaaattct
     4141 ttaggtaaaa aattctgtag aagatgtgaa tattgtatgc cttgtgctgt aggaataaat
     4201 ataccattga gttttttatg tgaaggttat tatactagat atggattaaa agagtgggct
     4261 aaagaaaaat atgaggttat ggatgttaaa cctactgagt gtattgactg tgggctttgc
     4321 gagagtagat gtccatatga acttcctata agagaaatgc ttaagactgt tgttgaaaag
     4381 ctaggataga ttttatttat tgaaatcggt gtattaaatt tactccgatt tctttttatt
     4441 agatgtggtc ttatatacaa atattaaatt tacatagtta tagctattat ataaatttaa
     4501 tatttgttta atacttatta aatatttgct taatgctttt taaaaacatt tgtttgagta
     4561 tagacataac acaattatta ttgttgtatt agtatagttt caaatactgg agtatcaata
     4621 taatgtcgaa taacacataa aatctttatt tttcaaaaat attataggat atatcgtatt
     4681 tagatactgg ataaagttca atttcaaccc tagggttatc tttatctata aaatcaaata
     4741 aaaatactgg cttaagttgt ttatcatttt ttattattcc acttttttca ataccatcac
     4801 aaatactttt tggataatta tgtaaatccc ccattctctt atttggaaag aataatttca
     4861 agactgttat tagattttct tcaacagttt ccccttgata ctgttggttt ataaaacctg
     4921 caatcatgtt ttcgtatgca agatacttag aatattttcc ctttgaaggc atccatgctt
     4981 gaccatttat gttatgaagc ttaaaattag atttactaat aggtctacct ggaattgtta
     5041 ttttaatcaa atttttcacc atccttatac agtatttatt ataatctgta tatgatttaa
     5101 aagcaaattg ttatttaaag tgtttttaca gggcttgttt gtaaaatttt ccactttaga
     5161 tgtagtaata taagataagt attttgctta caaattttaa aatttgatat atgatggaca
     5221 tattcatatt agactattat aagaccttca taatattaat aagtataggt ttagtaaaga
     5281 gagtaaaaag aaatccatat aagccaagta aagacttaat aaatggcaat gtaaatggga
     5341 tattaattat aagaaaaaat caatcttttt gcattaacta taggaagaat gtcaaaatat
     5401 aactaatgat atttgaagtt ttagagttta tattaaaagg taagaaggtt aagtgggtaa
     5461 aaaaggttga atagattaaa aaggctaagt atctaaaaga gttaagtagg taaaaaagtt
     5521 aaataagtaa atgaaaaaag ctaagtatct aaatgagtta gtagataaaa aaattaaata
     5581 gataaaaaga ttaagtatct aaaagcgtta gtagataaaa aaattaaata gataaaaaga
     5641 ttaagtatct aaaagcgtta gtagataaaa aaattaaata aataaaaaag actaagtatt
     5701 caaaagagtt agtaggtaaa ataattaaat agataaaaag actaagtatt taaaggagtt
     5761 aagcagataa aaaagttaaa taagtaaatg aaaaagacga agtatctaaa agagttagta
     5821 aataaaaaag ttaaataagt aaataaaaaa gatgaagtat ctaaaagagt tagtaggtaa
     5881 aaaactaaat aggtaaaaag acgaagtatc taaaagagtt agtaggtaaa aaagttaaat
     5941 aaataaaaaa gacgaagtat ttaaaagagt tagtagctag aaaaggttaa aaagactaaa
     6001 aaaattaaat aaatttaaaa agtcaattag attaaaaagg aataatttta tggataataa
     6061 ggaattaaaa gcattttgta atttaatagg attaaactgt gtaggagtag caggggtaga
     6121 taagtatgat aatttagaaa agatacttaa ggacagacaa caaaaaggtt atttaactgg
     6181 tatggaagaa ccaataattg agaacagaat taacccaaga catataatga aggatgctaa
     6241 ctctataata gtctgtgcat ttccatatca tataaataga caagaaaaga ataaaaattc
     6301 aaatttatca aaatattgtc atggaaagga ttatcacatt gtagtaaagg actttttaca
     6361 acaaatttgt gattatattt ctaaaacagt aacagatttt aaatacaagt tatttgttga
     6421 taatggacca ttagttgaca ggtatttagc ttatttatca ggaataggat attttggaat
     6481 aaataataat atcataactg atgaatatgg ttcttacgta tttatagggt atatagtaaa
     6541 taattataaa tttgaagaag ataaaccact agaaaaaagt tgttttaaat gtggtaagtg
     6601 tgtaaaatat tgtccgggaa atgctatatt gggaaattat gatatgaacc ccaaaaaatg
     6661 tttatcatat ataactcaaa aaaaaggaga tttgtcagag aaagaaaaag ttgttttaaa
     6721 gaatggcaaa aaggtatttg gatgcgatat ttgtcaagaa gtatgtccac ataatgttga
     6781 aatgccaaca actcatattt tggaatttaa agaaaatata atagattatt tggattatga
     6841 tgagatacaa aatatttcaa ataaagaatt taaaaggaga tatggagata gagcttttag
     6901 ctggagaggg agaaatataa taaagagaaa tatggaaata attttagata agtatgatga
     6961 ctaataataa gagtctgttt catatacttt aatgtgtatt tactttaaca cttgttgtgt
     7021 aaaagtcata ttatatcatt aaagttaaat atggggaggt gggggaatat tgaaatataa
     7081 taatacatct gaaataaatc cactagatta tatattgggt caagaaagag ctgtagaagc
     7141 tatggaatta gggttgaaaa taaataatcc agcgtacaac atatatatag ctggtgaacc
     7201 tggaactggt aaaagtactt atgccttaaa ggttttaaat gaatatgcat ccaaaaaaaa
     7261 tacacataaa gattggtgtt atatatataa ttttgaaaac cctagagagc ctattatagt
     7321 tgaattagaa aaggggtttg gaagagaact taagaaagat atggagaaat taattgaaag
     7381 tcttttagaa gattttaaaa atgcgtttga aagtgaaaat tttgaaatag aaaaaaatag
     7441 acttctagat gaatatgaaa tagaaaaaga tatgcttttg aagcaaatta agaaatatgg
     7501 ggaagaaaaa ggatttaaat taaaacagag taaaatggga atagttttta tccctttgaa
     7561 agaagaggaa gatgaaagtg ataagtctga tgaagagttt tacaaagcta aaagagaact
     7621 agaaaatatg gcgatacagg tcgtatataa aattagaaac ttagaagaaa tagctgaaaa
     7681 agcagtgtta gagcttgaag aagagatagc taaacttgta gtagaacctc atatcaagag
     7741 attgtgtgaa aaatatgaaa attatgataa gattcaaact tatctggaaa atataaagaa
     7801 agatataata gaatatatgt atttgttcta tttagatgaa gaggagttaa aagataagta
     7861 tgataaagag cattttatta aatacaaaat taacctattt gtagacaatg aaagctcaaa
     7921 gaataaagaa tcagcacctg ttgtagtaga gataaaccca agtccagcaa atctatttgg
     7981 aaaggcagag tatgattatg caaatggaaa tataaagact gatttcacaa aactattatc
     8041 aggagcattt catagggcta atggtggtta tttagtactt tatgcagacc aattactaaa
     8101 atatacaatg tcttgggaaa ttcttaaaaa gactttgcag actaaaaaag taatattgga
     8161 aacacaaaca gcaattaaac cagaaaatat gccattggat gttaaattag tactgattgg
     8221 aagccactat atatatgata ttttatatag atatgatgaa gattttgaaa aatattttaa
     8281 agtatttgta gactttgata gtgaaatgga taaaaatgaa gataatgaag aaggaatagc
     8341 aagatttata gcataccaat gtgataagaa taatttaagg cattttacta aatcagcagt
     8401 tgaagaggtt attaaattta gcactagatt atctggtgat ttagaaaaac tatcaactaa
     8461 gtttaataaa ataatggaag ttgtaataga aggaagtgct tatgcagagt ttagaaattc
     8521 ggagtataca gagcagtgtg atgttaaaaa agcaataagt gaaaagagaa aaagaatcaa
     8581 tagagtggaa acacatatgg atgaatcaat tgaaaatgga tttactctaa tagaaacaga
     8641 gggaagaaaa gtaggagtga taaatggtct gtcagtctta agtacaggag aatactcttt
     8701 tggtagagct tcaaggatta cagcgaccac tagcccagga agtaaaggca tagtaaatat
     8761 agaaagagaa gtaaatatga gtggctctat acataacaaa ggagtgctta tattaggtgg
     8821 ctatctttct gagaattttg cacaggattt actattgtca ctaaacgcat atgtttgttt
     8881 tgaacaaaat tatggtggaa tagatggaga tagtgcatct tctgcagaat tatatgtact
     8941 attatcatct cttagtggag tatcaattaa acaaaatatt gctgttacag gttctataaa
     9001 tcaaaagggt gatatacaag tagttggtgg tataagtgaa aagatagaag gattttattc
     9061 tatatgtaaa actaaagggt taaatggtga gcaaggcgtc ataattccta gaaaaaatca
     9121 aagaaatctc gttttatcag atgaagttaa tgatgcagtg cgagatggaa aatttaagat
     9181 atatactgtt gaaagagtag aagaggctat agagatattg acagatgtta aatttgaaga
     9241 aataaagaaa ttggtaaaag aaaaattaat atctttcagc aagatacaaa ctgtgtcaaa
     9301 agaataagtt tattatagaa tttaatgggt atttatgtat aataaaaagc aagaacttta
     9361 ataggttttt gctttttttg taaatatata taaattaaat tcttattttg tatgttttat
     9421 atattgatgt ttattttttg ttgatatgac taaactattt ataaatagtg ttttataaat
     9481 tggtatttta aagggggata attatgaaaa atgtaaatct aaaagagata aatgaaaaag
     9541 atgtaattgt ggaagaagat ataaatctaa aaggaatgga taagaaagat gtaattgaga
     9601 aggaagaagt aaataaaaaa agtatagata agaaagatat aattgagaaa gaagagatag
     9661 ataaaaaaaa tgtaaagaag gttgaggttc aaaaagaaat agataaaatg attagtaaaa
     9721 aagatgagat aattgatgag aaagatatag agaattttag gtatattgaa gaagataaaa
     9781 aaagtatcaa agtaagtaaa aaaagtacta aaaaaggtga aacggagaaa aaaacaaaga
     9841 aaagtagttc taataaggct acacaaaaac aaagcaaaga agttaaaagt aagtctaaaa
     9901 ttaaaagtga atcagccaat aaaaaagatg taaataagat tttagatgag ttggaaaaat
     9961 tgtacccaga tgctaaatgt gagttaaatt atgggacagc ctttgaactt ttaatagcaa
    10021 ctattttatc agcacaatgt actgatgtaa gagtgaataa agttacttca gagttgttta
    10081 aaaagtacaa cacagctaga gactttgcta atttgagcat tgaagaaatt agtaaagaga
    10141 taaagagttg tggtctttac aaaagcaagt ctcaaaaaat aaaggataca tctgaacagc
    10201 tatgtgaatt atatgatggt gaagtacctg atagcttaga aaagcttata aaattacctg
    10261 gtgttggaag aaaaacggca ggggttgtac ttagtaacgc ttttaatcat ccagcaatag
    10321 ctgtagatac tcatgtattt agagttagca atagaatagg aatagtggac gagccaaatc
    10381 ctcaaaaaac agaatttgca cttatggaag ctataccaaa agaaagatgg tcacactcac
    10441 atcatgtatt aatttttcat ggaagaagaa tgtgtaaagc tagaaatcca gaatgtgcaa
    10501 gttgcccaat aaaagaagat tgtaattatt ataaagaact taatgaaacg aaataacttt
    10561 tagaaagtaa gttgtacaag agggattttc tgatataata atacttgtga aaatgttaga
    10621 taaaaaacta ttatatacat gaaataaaaa ttattattaa cattatttta tataatagta
    10681 taatattatt gttattctgt ttataaaata aaatttgata aataaaagcg atacttaggg
    10741 aggattaaaa tgtcattaaa tatagtatta gtagaaccag aaatacctca aaatacagga
    10801 aatataataa gaacctgtgc agcaacaggt tcaactttac atttagtaaa accactgggt
    10861 ttttcattgg atgataaaca cctaaaaaga tgtgggcttg actattggga tatagctgat
    10921 atacaatatt acgatagctt tgaagaatta caagaaaagt atccaaatag taaatacttt
    10981 ttttctacaa caaaggctaa acagtcacat tcagatgtga aatatgaaga aaattgtttt
    11041 atagtatttg ggaaagaaac aaagggatta ccagaacctt tgttgaaagc aaatatggat
    11101 acttgtatta gagtgcctat gttggatgtt gaaaaggcta ggtcattaaa tttatcaaat
    11161 tcagttgcta ttgtagtgta cgaggcttta agacaaatag gatacccaaa tatgaggtag
    11221 tttcaacaag cctaaatgag aaaggagcat acttaatgaa aaatctaaag ataatctgtg
    11281 acagtttatc ggacgtgcca aaagatttgt tagataaata tgatatagat atagtttcat
    11341 taacagttat actggaggaa agagaataca aggatagaat tgatatatct ggagatgaat
    11401 tttataaaag gcttagagaa gaaaatgtat atccaaaaac ttctcaagct acttatgccc
    11461 aatttaagga aatttttgag aaatatacaa atgaggatag agagatactt tatatagcag
    11521 ggtctgccac agctacaggg acgtatcaaa gtgctgtaat ggcaaagaat gatgtggatg
    11581 gtaaaatata tacatatgac tctaacttgc tttgtatggg aactggttta cttgtaataa
    11641 aagcaggaga acttgcaagt gaaggaaagt ctgttgatga aatattacct gttttggatg
    11701 aattaaaaga gaaggctttt gttatatttt cagtagatac attggaatat ttacaaaagg
    11761 gaggtagaat atcatctact aaggcagcaa taggaagtat attaaatata aaaccgattc
    11821 ttgaaataag agatggacta gtttcgcaaa tatctcaagt aagaggaaag aagaatgtaa
    11881 tttctaagat gatggaattg ataaaatcaa attgtgggga tgatttatca gaccaaattg
    11941 tgtatatagg atatagtgat gattttaagg aaaaagaaca attatctaag gttatgcaag
    12001 atgaattaag acctaaagaa attagatttt tccaaattgg tgtgtgtata ggtgcgcatg
    12061 ctggtcctgg tgtcactggt atcatatgct taaaaaataa ataatatatt tttggatgac
    12121 attatttatt tttttattaa attaggatag aatattaatt attgagatgt taaattggat
    12181 ttattttgga tgaaattaag tgttgatata ttagatttag tttgtaggga agtgtgtaaa
    12241 tagataagaa tctattgcac acttttttat agtaagattt attaaaaaaa tttcaaaatg
    12301 tacttgtaaa ttgtatataa aaaaattata aataggatgt aaaagtaaat aggatgtaaa
    12361 agtaaatagg atgtaaaagt aaataggatg taaaagtaaa taggatgtaa aagtaaatag
    12421 gatgtaaaag taaataggat gtaaaaatat cgtaaatgta tgaggagtga aatttaatgc
    12481 gtttcaaaag aatattgata ttgatgttaa ctgtagcaat gatagctgga atgttggttg
    12541 ggtgtgctaa taacccaagc aataatccta gctctagcaa agataataaa agtgatataa
    12601 gcgataaaag taattcaaaa aaatctgtat caatgatact agatattgaa ggaacaaata
    12661 atgaagctat gaataatgca gctttattag cattaaacaa tgctcaaaag aaacttaata
    12721 tagatacaaa caaagtggaa tcagatgatt cctcaacttt ttctaatagt atagatattt
    12781 tatgtaatga caattacgat ttaattatag ctgtaggagc tagatttgca aaaccattgg
    12841 agatggtagc caagaaatat ccaaagcaac aatttgcaat tatagactat gagtatgaca
    12901 agcaaccttc taatataact tcaatttctt atgaagataa taaatctgga tacctagctg
    12961 gattaattgc tggaaaaatg actgaaagcg ataaggttgg ttttatagga ggaataaaga
    13021 gttctagtag ggataaattt gaatcaggtt ttagagaggg tgttaaattt tctaatagta
    13081 gtataaagga tatttcagtt gagtatgcag atgtttttaa agatagcaaa tcagtagaat
    13141 ctatagctaa aaaaatgatg gataatggag tggatattat tttttctact actgaagatg
    13201 attcaaaagc tgtaatagat gcagtaaggg caaaaaataa aaaagttata gcaacgaata
    13261 aagaccaaca tgagttagct ccagaaaatg ttataagttc tattgtaaaa gattttgaga
    13321 atcctactta taatctgata caaagttttg taaaaggaaa ttataaaggc ggaaaagtta
    13381 tagaaaacac agttaagtct ggaagtactg ggcttgccca aaatagttct caaaatattc
    13441 ctccagatgt gttagaatat gttaataaga acaataaata atctactata atatccaaga
    13501 atacttatga ctttagttat gaggtgaatt ggatgttata atagacatat atagaaagac
    13561 gtatgcagag gtaggcagac ctatctaaat cagggagtga attttgtgaa aataaaaagg
    13621 atattgatgg cttgctttgt agtagttttt acaattttta tattagctat atgtaacttt
    13681 gattttggat ttgataaagt aaataagaat aattctaata tagatagtgc taaatcgaaa
    13741 cagagtgagt ctaaaagtgt accaacttca gcagaaaagg ataaattaaa tagtaaaagt
    13801 aagaatgaag aagatgacac agaagatgat tcatatgtaa gaaaaactat tggagtattg
    13861 ttagataccg aaaacttaaa taacgaatca tataataata atgtactatc agccttaaaa
    13921 agggctgatg aagattttga tgttaggata aaacttgaaa aaccaaagac ttctaaaagt
    13981 tttaaagaga gtatagaaaa actacataag tatgaaccag atttgataat agcagttgga
    14041 gctagatttg cagagccaat taaagaagct gctactaaat atcccaaaca gcaatttgca
    14101 atagttgact atacttataa agttcagcca gctaatgtaa tttctgtatc atttcaagat
    14161 aatgttactg gatatttagc agggcttatt gcaggtaaaa tgactgatac tgggaaagtc
    14221 ggttttatag gcggagttga gggttctagt agagataagt atgaatctgg atttagaaag
    14281 gggcttaaaa cttcaaataa agatgctgag ttactagtta agtattcaaa tatatttgaa
    14341 aaccctgaat cagctagaac tattgcaaag gaaatgagag attctgatag agatgttgta
    14401 ttttcagcta cagaagatga tagtaaaaga gctattcagg tagcaagaga aaatggtggt
    14461 aaagttatag ctattaataa agaccaatac gatatggctc cagataatgt aataagttca
    14521 attataaaag atgtagaaca acctgtctat gaactgataa agaacgttgc aaaagatggt
    14581 tttaagggaa gtaaagtaat ggaatttaaa ataaaagatg gagaacttgg cttgacttca
    14641 aagagcagta gaaatatacc accagatgtt ttggaataca taaaaaaaga gtataacaaa
    14701 gtgaaagata catattaatt attgatgtag aagattataa aaatatactg tttaagttta
    14761 gattagcaat aaaaagatat atttattaaa tttgtgctaa ctgtacaaaa tatattgatt
    14821 aagtttagat tggtgataaa aagatatatt tattaaattt agtctaactg tacaaaatat
    14881 attgattgag tttagattag caataaaaag atatatttat taaatttgtg ctaactgtac
    14941 ggaatatact gcttaagttt agattagtga taaaaagata tatttattaa atttgaacca
    15001 actgtacaaa gctatactaa ttaagcttag attaactata taaaattata acaataagtg
    15061 tgtgggcaga aaaattctgc tacacacttt atttatattc atataaaaaa attatcaaaa
    15121 aaaagaaaaa ggagtaaaaa atggaattta caagagaacc atttatacta gatgacgatt
    15181 tgtgtctagc tttggtggac aaaagaatta gttatctaat ggaaaatgaa ttaaaattaa
    15241 gaaatataga gataataaaa acaatagaat gtaaagaatt atatgaaagc ataaaatatc
    15301 acccggatat atgtatttgt aatttaggaa aaggagatat aattgttgcg ccgaatgtat
    15361 ataatcaata caaagagtta ttaagtagat ataaatttaa tataattaag ggagaagcta
    15421 atttattaaa tagataccct tatgatatac cttacaatat tgcgatagtt ggtgactatg
    15481 ctatacacaa ttttaagtat actgacaaag caatattaga ttatattgaa aataataaac
    15541 ttaaaaagat aaatatagag cagggctatt ctaaatgctc aatctgtata gtagatagaa
    15601 cgtctataat aacatcagat aaaggtattt ggaaaagtat gaagaataca gaaatagagt
    15661 gtttattgat agaaaaaggt cacataaatt tatttgagat gaattatgga tttataggtg
    15721 gatgtacagg gttgatttct aaggataaac tagctttttg tggagatgtt aaaaaacatc
    15781 ctgactatga acgtataaaa agttttgtag aaagtaaaaa caaggagata gttacactta
    15841 gctgtgaaaa tttactagat ataggttcta tagttcctct catgacaaga aaggagcgat
    15901 aactttggtt gagaaggata atatttcttc catgaaattt ttgaggaata ataatgtaaa
    15961 cgatagatta atatatacga atgacattgt agaagtagtg atagattctg caaagcaaga
    16021 agtgttaaat aaaaaaatac ttaatggtac taaagctgaa caaagagctt taaatattga
    16081 tgagatatca acggaaataa ctagtatgat aataagtata atgaaagaga aacttttgaa
    16141 agaatacatt ctaaaatgct atgggcgtac atatcctgaa gataaaaata atatatacat
    16201 atattcactg aacatatttg ctaaaaaaga gatttttatg agagaaacag tatttactag
    16261 ggtgagaaat tatatagaag aaaatgattt tataaatgta gaaggtttta ttagatttag
    16321 aatgagagaa tttatgaaat atatttctgc tataggtgat attgcagttg aggagtattt
    16381 aataaagaaa gaccaagatg aatttataag agttttaaaa tactttatag atactcaaga
    16441 agaaaagatt gatttgctta aagttcatat aatggaagat aatacatttg ttttatacga
    16501 taaaaatgga aataagatag acagtataga tgatgaagaa attataaata tggtaataag
    16561 agaaaattta aattatgagg actttttaat aagtacctta ttaactctat gtcccaaaaa
    16621 aatagaaata ttggatttgt taaataataa ttgctcaaag gaaattatag atactgtaga
    16681 agcaattttt gaaaataaag ttagtataat tatggaaaac tagaatctaa gcatataaaa
    16741 gctagccata taaaattaaa aaaattcttt ttaaatcgac acaaaagact tataattgta
    16801 tataaagcta tagtataact agagttatta agtataaaga atatctactt gattaatgca
    16861 atatagctgg caaatataca attgataagg agtaatttta taatggataa tttaattagg
    16921 atactgatat tagctgttat tggtggattt ataggttatg ttacgaatgt agttgctata
    16981 agactgatat ttagaccaat agagcctatt aaaataccta ttttaaatat agaaatagta
    17041 ggtcttatac caaagagaag agcagaaata gcagcaaatg taggagaaat aattcaagaa
    17101 gaatttcttt ctatggacga aatacttgca aatatcatta ctgacgaaga taaggaagaa
    17161 gttgtaagat acataaaagc aagggtaaaa attataatac atgagaaagt ttcatttatc
    17221 ccgagtggta taaaaaacat gatacaagat tatctaggag agataattga gtctgaagtt
    17281 aagcaaagta tagatgaatt gagtaaaaat ataataaata aagcaaatga acgtatagat
    17341 atacaaaaaa tggtcgaaga taagattaat gaactagatt tatatgaatt ggaagagata
    17401 ataataagaa tagctaagaa agaattaaaa catatagagt ttttggggct tgtcttaggg
    17461 tttttaatag gtatagctca agggttaatt actatgttta tttaaatata tgagaagtag
    17521 catttattta agtactaatt atatttacct atttgagtga attacttatt aatagtattt
    17581 agatattaat tgtatttacc tatttgagtg aattacttat taatagtatt tagatattaa
    17641 ttgtatttac ctatttgagt gaattactta tt
//
LOCUS       DAELXX010000085        17503 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.085, whole genome shotgun sequence.
ACCESSION   DAELXX010000085 DAELXX010000000
VERSION     DAELXX010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17503
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.085"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            228..431
                     /locus_tag="KPH29_002676"
     CDS             228..431
                     /locus_tag="KPH29_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1468261.1"
                     /translation="MISYAPLWKLLIDRKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKGI"
     gene            595..1749
                     /locus_tag="KPH29_002677"
     CDS             595..1749
                     /locus_tag="KPH29_002677"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBG1468262.1"
                     /translation="MKKISILGSTGSIGKQTLDVVREHRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELVNITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFRVEQENIDVVVHPQSIIHSMVQYADSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            1762..2766
                     /gene="rseP"
                     /locus_tag="KPH29_002678"
     CDS             1762..2766
                     /gene="rseP"
                     /locus_tag="KPH29_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBG1468263.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSNKVVNVTPKNNNGKYEIGIQPQREKDFLASIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            2949..4004
                     /gene="ispG"
                     /locus_tag="KPH29_002679"
                     /gene_synonym="gcpE"
     CDS             2949..4004
                     /gene="ispG"
                     /locus_tag="KPH29_002679"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBG1468264.1"
                     /translation="MSYERRKTREVSVGTVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSRERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKHGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
     gene            4352..5176
                     /locus_tag="KPH29_002680"
     CDS             4352..5176
                     /locus_tag="KPH29_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468265.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLASTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSIWKNSAYENWLKSFQGKEVSNSVLENYYK"
     gene            complement(5649..6772)
                     /locus_tag="KPH29_002681"
                     /pseudo
     CDS             complement(5649..6772)
                     /locus_tag="KPH29_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
     gene            6969..7193
                     /locus_tag="KPH29_002682"
     CDS             6969..7193
                     /locus_tag="KPH29_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468266.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
     gene            7246..7770
                     /locus_tag="KPH29_002683"
     CDS             7246..7770
                     /locus_tag="KPH29_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG1468267.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(7875..8021)
                     /locus_tag="KPH29_002684"
     CDS             complement(7875..8021)
                     /locus_tag="KPH29_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468268.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(8160..9086)
                     /locus_tag="KPH29_002685"
     CDS             complement(8160..9086)
                     /locus_tag="KPH29_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBG1468269.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            9277..11148
                     /locus_tag="KPH29_002686"
     CDS             9277..11148
                     /locus_tag="KPH29_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBG1468270.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNASCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYISLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            complement(11240..12175)
                     /locus_tag="KPH29_002687"
     CDS             complement(11240..12175)
                     /locus_tag="KPH29_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBG1468271.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
     gene            12695..13207
                     /locus_tag="KPH29_002688"
     CDS             12695..13207
                     /locus_tag="KPH29_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBG1468272.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKEAKMAIICKKIYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
     gene            complement(13362..14423)
                     /locus_tag="KPH29_002689"
     CDS             complement(13362..14423)
                     /locus_tag="KPH29_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HBG1468273.1"
                     /translation="MIIKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVI
                     SGVQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEA
                     VKAGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIV
                     VEGPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSS
                     SDVKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAI
                     RNKFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAY
                     KIDKLSTVEDVINELISEL"
     gene            complement(14547..15440)
                     /locus_tag="KPH29_002690"
     CDS             complement(14547..15440)
                     /locus_tag="KPH29_002690"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBG1468274.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIHSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQNYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            complement(15450..16931)
                     /locus_tag="KPH29_002691"
     CDS             complement(15450..16931)
                     /locus_tag="KPH29_002691"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBG1468275.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISSDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKKFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSEINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     misc_binding    complement(17039..17270)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         6684 a         2071 c         2718 g         6030 t
ORIGIN      
        1 agatatataa tagtaaatat tttttatata gttatgtttt agtaaaataa tgagataaat
       61 atatataaag cttaattttt gaacttatgg ttttgtttta ttttaacata tttatattta
      121 ataaactata attgaaatct atttagataa ataattattt atctaaatag atgaaaattt
      181 tccaaaatag ataaaatgtg atagaatata ttttgcgagg tgataacatg atttcatacg
      241 ctccattatg gaaattgtta attgacagaa aaataaagaa aatggaattt gtaaatatat
      301 caggtataag tatttctgtt ttaggcagac taggaaatga taaatctgtt tctatggata
      361 caatggagaa gatatgttta gcactagact gtaaaataga agatgttgtg gaaataaaaa
      421 agggcattta ataatactag aatattttat aatattccac aagatacaaa aaataataga
      481 ttaattttaa tctgtggaat aatatatgaa atttctatga atagtaaaaa taattgtttt
      541 agtagtgtta taatgttttg acagggtata tttattatta gaaaggatta ttatatgaaa
      601 aagatatcaa ttttaggttc tacaggctct ataggtaaac aaactttgga tgtggttaga
      661 gaacatagag ataagtttga gatagttgca atatcagcta acagtaatat tgaattattg
      721 ctagaacaaa tagtagagtt taaacctaaa tatgtaacag tttttgagga aaataaggca
      781 ttgaaattaa aagaaatgtt gcctaaaaat atagaaatag aagttttagc aggaatggaa
      841 ggattaaaaa taatatcatc acttgatgaa gtggatgtcc ttttaactgc tgtagtaggt
      901 atgataggat tagtcccaac tctttgtgct ataaagaagg gaatagacat agcattagcg
      961 aataaagaaa cgttggtaac tgctggtgaa cttgtaatga aagaagcaga aaaatacaat
     1021 gtaaatattc ttcctgttga tagtgaacat agtgctatat ttcaatgtct aaatggagaa
     1081 aataaaaaaa atatagaaaa gataatactt acagcatctg gtggtccatt tagaggaaag
     1141 aaaaagggag aacttgtaaa tataactaaa aatgaagctt tgaagcatcc aaattggagt
     1201 atgggaagaa aaataagtat tgattcttca acgcttatga ataaaggact tgaagtgata
     1261 gaagctagat ggttgtttag ggtagaacaa gaaaatatag atgtagtagt tcacccacaa
     1321 agcataattc attcaatggt gcagtatgca gatagctcaa taattgctca attagggtgt
     1381 ccagatatga gactaccaat acagtatgct cttacatatc cagatagaat ggaaagtagc
     1441 tttgaaagaa tgaatttttc aaaatttagt actttaactt ttgaagaacc agatttagaa
     1501 acttttccat gtttaaaatt ggcctatgag tgtttaaaaa tgggaggaac ttattcttct
     1561 gttttaaatt cagcaaatga agttttagta agtgaatttt tagaggataa aattggtttt
     1621 tatgacatac catattacat agagaagact ttagaagttc atagtagcat aagcgaacca
     1681 acattagaag aaatcttaga aacagataga tggagtagag cttatgttgc aaatctgata
     1741 aaaaaatagg aaggtgaatt tatgactata atagccgcat taatactatt tagtataatt
     1801 gtattaatac atgagttagg acattttata tttgcaaaaa gaagtggtat caaagtaaat
     1861 gagttttcta taggtatggg gcctaagata tatagtgtaa aaaaagatac agagtattct
     1921 ataagagcac ttccaattgg tggatatgtc agcatggaag gtgaagatga agaacaaata
     1981 agtccaaatt cttttggaaa caagtctata ttacaaagat ttagtactat tgtagcagga
     2041 ccaatattca atataatatt agcagcaata cttttagttc cagtattttt gtatataggt
     2101 tctccaacga ctaaattagg aaagataatg ccagatactc ctgcacaggc tgtagggctt
     2161 caagtaggag ataagataaa taaaattaat ggtaattctg ttaagacttg ggatgaagtt
     2221 gcaaatataa taaacacttc ttctggtgga gaattaaaac ttagtataac tagagatgga
     2281 agtaataaag ttgtaaatgt aacacctaaa aataacaatg gaaagtatga aatagggatt
     2341 caaccacaaa gagaaaaaga ctttttggcg tcaatagtaa atgcatgtaa aactactgta
     2401 gatatgacaa aacagatgtt aacatttcta ggtcaaatga ttacaggacg tgtaccagga
     2461 gggattggca atgctgtagc aggaccagta ggtgttattg gtatggtatc agatgctgct
     2521 cgtacaggca tcataaatgt agtgtattta gcagctgtca taagcttaaa tttaggtata
     2581 gtaaatctat taccaatacc agcacttgat ggatggagaa tacttatgct attactagaa
     2641 gctgttagag gtggtaaaaa actcgaccca aacaaagaag gcatgataaa tgttgttggt
     2701 tttggagctt taatgttatt tatgcttttt atcacatata aagatatatt aagattattt
     2761 caatgattta gttttttaga gattttaaga aaataggcta tgtagtttta aataagaata
     2821 aattaatttt tatgtatgaa atttattctt atttaactag atagcttttt aatttataat
     2881 atacatgtag gttataatat tttactattt aaagagattc aatataaaat aatatataga
     2941 ggtggaatat gagttacgag agaagaaaga cgagagaagt aagtgtagga actgtaaaaa
     3001 taggaggaga aaatcctata agtatacaat ctatgacaaa tactgataca agagatgcag
     3061 atgctacaat agctcaaata aagagactag aggaagctgg ttgtgatata gttagagttg
     3121 ctgttcctga tattaaagct gctaagaata tagcaaagat aaagtctagt gtaaatatac
     3181 caattattgc agatatacat ttcgattata agctagccct tgaagctata gaacaaggtg
     3241 tggatggaat aagaataaat cctggtaata ttggaagtag agagagagtt aagatggtag
     3301 ttgagaaatg taaggaaaga aatttaaaaa ttagaattgg agtcaatgga ggttctttag
     3361 agaaagaact tttaaaaaag catggctctg caactgctga agcattagtt gaaagtgcaa
     3421 tgggacatat taagattctt gaagacttag attttcataa tatagttata tctctaaaat
     3481 catccgatat ttataaaact gttgatgcat atgaattaat atcaaaaaaa gttgattatc
     3541 ctcttcatat tggaattaca gaatctggaa gtgttcacaa aggaacaata aagtcttcta
     3601 taggggtagg agcacttctt cttaaaggaa ttggagatac tgttagaata tctttaacag
     3661 gcgaccctgt tgaagaggtt attgttggta aacagatttt aagaagtttg ggacttttaa
     3721 atgataagat taaagttata tcatgcccta catgtggtag atgtaatata gacttaatta
     3781 gtgttgtaaa tgaggttgaa gaaaagattg gtagtatgga aaaaaatatc actgttgcaa
     3841 ttatgggctg tgctgtaaat ggacctggtg aagccagaga agcagatata ggtattgctg
     3901 ggggtaaagg tgaaggtctt ttatttaaaa agggtgaaat agttagaatg attgatggta
     3961 acaaattagt tgatgagttg ctagaagaga ttgaaaaatt atagtataca attcatatat
     4021 atattaattg actagatttt taaatacttt tgtaataagt ttttatttac agtatttaat
     4081 tatctagtca ttttttattt tataaacttc attaaatgta agaaacttat attctaattt
     4141 gactatatat gtatgagtta aatcatattg taaatttata aaattaaaaa aagatttaat
     4201 atagtgaaaa tttccatcaa tagtattgaa acttgatagt aactatgata ggatatagtt
     4261 actagaacag ttggtaaatt atgttaacat agattttaat gttttggtaa attgttttat
     4321 tataagttaa tagaaaaaga ggtgaagaac tgtgagatat aaaaaaatat ctagcttacc
     4381 tattttagtt gttgctttgc tgattactgg aacgacaaca acattttctc ttagtacgac
     4441 caattcagaa aaaacaaatg tatacttagc aagtacggta agtactgtca caaagaaagt
     4501 atccgatatt caaagtagta aaggtcttgt ttataatgag aatattccat tatttataaa
     4561 taatagcaaa atacagtatg atgatactcc atatcattgg cctagcaatg ttataagctt
     4621 aacaaatagc agtgaaaagg caattatgga ttatgagatt acatgcttag catatgataa
     4681 aaatggaaaa ccacttgagc tatattggga tgctcaaaat gtggcagcag atggcgaagt
     4741 aggaagtgtt ggatttagcc cagctggtgt agactatggt attgttacag gcatatctcc
     4801 tgtgtctcca aaatcatatt cacatactta tagaaaaatg caacaatcac caccacagga
     4861 tataataagt atgtttgaaa aacaacaagg caaggcatgg gtagaaaatt ggttgaaaga
     4921 atggaaacaa atggagaaag aatatgcaaa gcaaaatgca atagcaccag gtaagaatca
     4981 aaatgatgcc tttctattgt ttgataaatg gaaacaatca actggagagc atggtgtaaa
     5041 gtacattata tcttgtgtaa aacaagtaac atttaatgat ggtagtatat ggaaaaattc
     5101 agcatatgag aattggttga aaagttttca agggaaggaa gtttcaaata gtgtattaga
     5161 aaattactat aagtaatgtt taatgatagc aaaatgagta cgaatttaat taatgttaat
     5221 ttaattaaat aaggactatt ttgtttaaaa atatcagtgt tacaaggaaa tttttattgt
     5281 gacaaaatgg tatatattca aaataaaaaa gcatgtgtca agttttgata catgcttttt
     5341 tattttgaat atatgttttc tataaattta tgatttttta gttatgtaat tttatatgtg
     5401 aatttttaga tttttatata gatagtgaaa taatgtggaa gttttatgtt ttaaaaattt
     5461 tagttaaaac atttattaaa aaagataata gaaatgtttt aactaaaata gttagaattg
     5521 aaatgttata cagatagtat aagtactatc aaattatata agattgcttg aagttaaata
     5581 atttatttaa atagaaaaca gctgatttaa tatcagctgt atatgaataa aattttgaat
     5641 tgtagtgttt agttatatgg atataattca taataagtta gcaataaatc aaccatttta
     5701 ccataactct gagtaccttc agtaacgcca ttagctttta agtaagaatt gttaaactca
     5761 ttagaaattt cattaatttt accttcatat ttttgccaaa attcactttc attttttaaa
     5821 tctctacgga cagaatctga aatgccagca gaaatatcaa catatgcatt ataatcaacc
     5881 ttacttaaag cagaagcagt ataatttaaa gctaaaatat atccagaata gttaaaatca
     5941 atatgggggt gttttataga agtaagatac gctataaaat tagcctcatc ttcactagca
     6001 aatccccttt gatgtgctat ttcatgttca actgtacaag gaatatataa gtcaggtatt
     6061 gctatattta catttgcctc acctgtaaaa ggaaagtata taccagttat tcctgtatag
     6121 cacattagat ttgatgaaat aatatactta ggcttagaat aactaccact aacactaggt
     6181 aatatatcca agatattatc gtatcctagt tgagctctat ttatgacacc attataatct
     6241 gtattagatt ttacaatacc atttttatct tgtaatgtga gttttctagt ttcatttgct
     6301 ttaattacaa ggaatttata taatttagta agctctttaa cactatattg ttttgattgg
     6361 atagaggtat tgttttttaa attatagtta tttataagtg tggtttctag tggaatccta
     6421 ttatagttta ttccccaaag cactacaaaa agaaagtaga ctattgatac aatggataat
     6481 atatttaaaa tggaattttt taaaaatatg tttaattttt tttcttatta aaaattgtag
     6541 ataaagtaca acataaaaat aagaagatag atataactat tatgtacatt gtaatctcat
     6601 agagagaaaa tgggaaaatt ccagaaactt tactcaatat ttgtacaatt attttatcta
     6661 tgaattggga gtaatatttt tcaacaatat ttggaacttt gctggctata aaatttaaaa
     6721 gtaaagcaat aggaaagaga attaaagaaa aatattttgt ttttttgctc atatttcacc
     6781 tcacatacat tagtttatat aaacatatta tatatcaatt gaagaaagat tgtaaaataa
     6841 gtataaatat atctatttat aactttatac taaataaatg ataagttata atatattaat
     6901 atgaataagc agtatatata caactaataa taagaaaaat atcaattata taaggagtag
     6961 ataaaaatat gataataaaa cttactaaag acaattttaa ttttagattt aaacaatatg
     7021 aaggtatgtt ttttcatgct gaagtagttg aaggtatgga atccattaga tataaaataa
     7081 ctaaggataa agaaggtaca gaattaaaag aatttggaag tacacatata aaagataata
     7141 tgatatatgt acctcaggta gaagcataca tagatttgaa cagtataaaa taaagatgta
     7201 aaattttaat taaaaataaa gattatgttt gagggggaat ccaatatgag taagcctata
     7261 gatataacaa taagacctat aagaagagaa gatgcacaag cttttaatga aattagaaga
     7321 acatacagtg ttatgagaaa tactttggca ttaatgagtg acagagtcga taaaactgaa
     7381 gctatgttag cttctttggg agaaaatgat tatatgtttg tagctgaaac taataataat
     7441 ggagaaaaag atgtaatagg ttttgctggg ttacatgtaa attcttctcc acgattgaga
     7501 catagtgcag aaataggaat aagtgttgca gagaattatc agggaagagg agtaggtaag
     7561 aagcttatgg aacaactttt agatatagca gataattgga ttatgttaac tagagtagga
     7621 ttagaagtaa ttgtagataa tgaaaaggga ttaaatttat ataaaaaact aggttttgaa
     7681 atagagggaa ctaaaaaata tgctgtaatc agagatggaa aatttgaaga tgtttatatt
     7741 atgggaagat ataataaaaa tttaatttaa agaagctaat agagaagtaa aatagagtgt
     7801 ctttaataaa aaatttacat taaaaataat ctattttact tctctattgt attaaaaaac
     7861 ttatttttaa atgtttacaa aaacattcta tatacaaata aaattattag caatatcaac
     7921 gctattttaa aagctaaact agcaacagtt ttaatgattt ttacagaaac acctatagca
     7981 actatagcaa atactatttt aaagagtaaa tccatatcca tatttaagcc tcctattcta
     8041 taatacatat agatattatt gacaaatatc taggaaaaaa ttccatcaat atactacaat
     8101 aaaatgttta tatttgtaat ttattgaata tgttattata taatacataa aaaaataaat
     8161 caagataaga gtaaaatact tataaaaggt atagatataa ctgaaaatag tgttgtaaaa
     8221 aacacacatt gtgaagctag tgttatattg gagtcatatt gattagccat tatagcagta
     8281 tttgcagcag caggcattga agaaattact actggtatag ctaaaatcat cttatcattt
     8341 atccatcctt ttagaataaa atacacaatc acaggaagta ccaataatct tataaaagtg
     8401 actatatata attttttgtt gacaaaacaa tcgagagctg aagaattagc aagtaagctt
     8461 ccaatgataa tcatggatat tggtgtagtt atacttccca acatctcaag tggctcgttt
     8521 ataaattggg gtaacctaag tcctgttaca aataaaaaaa gtccaataac aatagctatt
     8581 gtagcagggc ttataagtga ttttattgaa aaacctctac ttgtactgcc tttagataaa
     8641 agataaattc ctaaagtgaa agaaaataag ttaaaaggca aattgaatat tgcagtatag
     8701 aatattgctt catgacctaa aactgcttca attacaggat aacccataaa agcaacattt
     8761 gaaaaaacaa ctatatactg atatattccc aaatcttttt tatcattaca ttttaaaatg
     8821 tatttcaata aaaatgcaat tataattgaa actatataca taatcaaaga tgtaaataat
     8881 aacaatagta ttttttgtat cattttacta tcaaagttaa tttgcatgga gctaattatc
     8941 attgatggta aaaaaactgt cattgtcaaa gttgaaagct tagatgtgca gtggtcatct
     9001 aataagttga attttctgac aaaatatcca actaaaataa ttataaataa aactgcaact
     9061 tggataaaaa tattgctgaa attcataaaa tgtccccccc tatatataca ttatatacca
     9121 attacataat aaaatctaat gattataaaa tttaaaattt aatgttacaa tatagtgaca
     9181 aaaggtaaaa attcaatatt atagcattaa ttatagatta taatgtataa taatgtttga
     9241 gagaatatat tttataaaat gggtaggtag cgaataatga aaaatacaaa attttctata
     9301 aaaaaaataa aaaatatatc taatgacccg aaagtcgtac atactaaggg tgccctcctt
     9361 gaaaagatgg gtaagatgga aagcgcaata gagttgtata agagtgctgc attggataat
     9421 tacgtaaaat ctcaatatac tttaggaaat atatatgaaa gtaaaaaaca atttaaagaa
     9481 gcagaaaagt ggtattcaat ggcatataaa agtggaagtg aagatgctgc ttttgattta
     9541 ggtaatatgt actataagtt agatggctat gaatatgcta tatattggta tgaaaagata
     9601 gcaaatttag gttatttaca agcacaaaat aatttaggtg tatgccattt taaaatgaaa
     9661 gattttgtaa ggtgtgaaaa atggttaaaa acagcagcag acaagaattt aggtaaagct
     9721 tgttttaatt taggggtatt atatactttt ttggagaaag atgatgaagc atatgaatac
     9781 tataaaaaag gttctgtgct tttagatgat gatgcaaaat ataatttagc aatattaaat
     9841 ggaaaaaaga aagataataa atcaacagta agtttatata aacagttgta caagtcaggt
     9901 catatgaaag gttgctttaa cttgggtatg attatggaaa taaatgataa tttagaagat
     9961 gcagagaagt attataaaaa gagtgctgat agagatgatg tgaagtctga gtataggctt
    10021 gcatatgtat atgatagaaa agaagagctg ggagatgcaa tttattatta tgagaaagca
    10081 atagaaaaaa atcatacact ttctaagtat agacttgcta atttatataa tagagaaaat
    10141 gagatagaga aggcaaaaac ttattataaa atggctgctg aagatgatat aacagaagca
    10201 aaaaataatc tggcaggtat atattttgaa gaaaaagatt atgaaaatgc tattaaatat
    10261 tatgaggatg ctatagctgt tggatgcaaa agttcccttg aaaatcttgg ggatttatat
    10321 tatcagaatc aagatataga aaaagcgatt tcgtattatt cgaggattcc aaataatgca
    10381 tcttgtcaaa tcaaattagg aaatatctat gaagatttaa ataatattga agaagccata
    10441 agttggtata agaaagcatc tgaaaatgga gatacgaggt ctagttatag attggggtgt
    10501 atatatgaga gcttaggaaa tacaaaaaat gctagaaagt attttgaaat ggcaagtagc
    10561 aaaaatcata tgaatgctcg tatacatcta ggtagaattt attttagaga aggtaaattg
    10621 gaagagtcta aaatgatgtt tgatacacct gccaatgaaa ataatgtgta cgctcaacat
    10681 atggtaggat taatttatga tatgttctat aaagattatg taaattctaa attttggtat
    10741 gaaaaggcta gagcacaagg ttgtgttgaa tcaatatata atttaggcca aatttattta
    10801 aaattaaatg atgacgcaga agctgagaaa tattataaag aaggtattaa atatggaagt
    10861 aagaagtgtg agtatatgct tgcaggtctt tattataaaa agagcctaga tatgtacatt
    10921 tctttagcaa atgaagagta cgataattgt gaagagatag tagaaggctt tcccaattta
    10981 aatatagact ttgatgaagt attaattcct ccatttaagt tgcaagaaga ggtaatagat
    11041 gaagaagagt atgtaccaat ttatatacta aatataaaag aaagtttaga ttcaatgcta
    11101 gaagggcttg aaactgaaat gattatagat gaagaacatg atagttaaaa agtattataa
    11161 gattatatag atttaaatat ataaaagcca ttaggataaa tgtaaatcaa ttaattctaa
    11221 tggctttttt attaatattt tataatagct tatttcttga catgaaaaat gtaatcacta
    11281 tacatattat agaggtaata agaagtatta tccaaaatcc gtttgggtta ttagaaaatg
    11341 gcatgttgat gacattcata ccaaagaatc ctgatattat tgtaggtatt gagaaaatta
    11401 gtgtaactac tgttaaaatt tgcataacat tattctgatt attacttata attgcactga
    11461 aagcatccat aattctactc aatatatctc catatatttt agccatatca agagcctgtc
    11521 tattttcaac tataacatct tctaataggt cttcatcatc tggatacttc tttataccag
    11581 cagttctaac cattttgttt aaaacaagtt cattagattt tagtgaggta gagaagtata
    11641 ccaaagattt ttctaattcc aataattgaa ccaattcttt gtttttcatt gatttgtaaa
    11701 tttctcgctc tattatgatt gtcattttat ttatttttct taagtaatgc aagtaataag
    11761 ctgcattttt gtgaagaatt tgaagcaaaa agcgagtctt tttaaacgta aaaaaatctt
    11821 taatatgacc aactataaag tcatttaaaa tcttagtctg tgcatcacaa actgttacaa
    11881 tagcttcttc agttagtata atacctaagg gtattgtagt ataatgagaa gaattagagt
    11941 cactttcgtc tactggtata tctataagta taagagtatg attgtcttca acatctattc
    12001 ttgagcgttc ttcttcatct agtgcagatt ctatgctttc tacatctata tttaataaat
    12061 tagaaatttc gttgatttca ctatgattag gttcaacaag atttatccaa cagccatctt
    12121 caaaaaaact aagtttctct aattttgaat ctatagtttt gtaatatctt atcatggcaa
    12181 cacatccttt attcagtttt atataaatct catcatttaa tagtatctat taattaacta
    12241 atttatataa ctgaatatat gtattagttt tgattttaga tattattaag tgatgaaaat
    12301 gtaaaaaatt ctctccgcaa cggactagta tccattgccc atcactctcc ttatttatta
    12361 ttttatatat agctatttat gaaatgcgta ttttaagcac tctttaatat aatacctttt
    12421 ccaggtatat aaatcaagtt ttgatttgat taaataatat ttttaccata atttacacat
    12481 aaagtatata agttatattt aatttgagta aagaatatat atattctgag tgaaattatt
    12541 aaatatatta aaaaataaga gtgtatatat atatttactt atttgcaata atatatttaa
    12601 aaaagtattt tattttcaaa actagacttc atttataatt tatcacgaaa ataaaaggaa
    12661 tataaatata agagaatgag aggttattag agatatgagt gattttaaag aaattttggc
    12721 agaagagatt aaaaataatc catttggatt gataggtaaa gattggacac ttataacagc
    12781 agaaaatgaa ggtaaagtga atacaatgac tgctagctgg ggaggacttg gagttatgtg
    12841 gggaaaagat gttgcttttg tagtaataag accacagcgt tatactaagg aatttataga
    12901 taatacagat aaattttctc ttacattttt tgatgaagat tttcgtaaag agctttcata
    12961 ttgtggaaag gtatctggta gagaagagga taagatatca cagattggat ttaatataga
    13021 acacttaaat gatacacctt attttaagga agctaaaatg gctattatat gcaaaaaaat
    13081 ttatagtcag aaacttgaac cccaatgttt tattgctgaa ggtatagatg gaagatggta
    13141 tccacaaaaa gattatcata ctttatatat agctgaaata accaatgtac ttgtaaaaga
    13201 agattagtta taagggttaa tactatctct aattaaatta aaatttcaat aaaaggaaat
    13261 tgacacaaaa aagctaagga cataaatatg tttataaaga ttaataaata acgtattaaa
    13321 actccttagc ttgataaaat tttaatttag ttttctttaa attataattc agaaatcaat
    13381 tcattgataa catcttcaac agtacttaat ttatcaattt tataggcatc atttccacaa
    13441 aataaaagtg aattttcaac atctccttta actgcattga ttagagcttg agaaatacag
    13501 taaggagttt cttttggatt acaaggaatc aaacaattat agcacttctt tatttctgga
    13561 cgatttattt taacttcttc tataaatttg tttcttatgg cgcgtccagg aagacctaca
    13621 ggacttttaa ctatatgaac atcttcttca ctagcattta tgtatgccat tttaaagttc
    13681 tcatgggcat cacattcgta agtagcaaca aatcttgtac caacttgtac accacttgca
    13741 cccatatcta tatatttttt aacatcacta ctagaagtta taccaccagc agcaactact
    13801 ggtatgtttc taccaaattt ttctccataa gtatttgcaa cctttaaaat ttcaacaaat
    13861 tccttatcat agtctataga atctatgtta tcaagttcct cgttagagta tccaaggtga
    13921 ccaccagctt ttggaccttc aacaactatt aagtcagcag tagttttctc ttttctatcc
    13981 cacattttta aaattacatt agcagctttt gcagttgaaa ctatgggtgc tatttttaca
    14041 ttacttccct ttactaatga tggtagctta ttaggtaatc cagctccaga tattataagg
    14101 tctacaccag ctttaacagc ttcctttacg tatgtttcat attctttcat agctaccata
    14161 aaattaatac caataatacc accatttgag atttctttgg ccttagaaat atgttttttt
    14221 atagctctta aatttgcatt tatagtatta gtttcaaaat cttcctcatc atatccaatt
    14281 tgaactccag aaattacacc aacaccacca agcttagcaa ctgcaccagc tagtgatgac
    14341 ctagatatac caacgcccat tccaccttga attataggaa cttttgcaac taaatctcta
    14401 attattaact cttttatgat aatccctcct caatgtaaac atgtttaaag aactagatta
    14461 taatacttaa tattattacc tagtcctaat tattatacta caaattataa taaaagagta
    14521 gttatatttt gtatagaaga cgataattat attatattta aaacagctcc attagaatcg
    14581 caacataact cttttatttc ccaatcagat tttagttttt gtaggtaatt tttcatatta
    14641 tccacaaaac tatcatcatt ttcttttatt aaagacatta tagtaggtcc agctccactt
    14701 aaaaatactc caatactatt taattgttca gatttttcaa caatatcatt gtagttctca
    14761 attagagttc ctctataatt ttgatgcaat ttgtcttgac aagctacttt aagtaggtca
    14821 aagtttttgt ttagtaaggc agatattagt aaagcaactc tactaacatt gaatacccca
    14881 tcagaatatg gtatttcctt tggtaaaaca cctctagctt tttctgttga taacttaaaa
    14941 ttaggtatca ttgcacaaaa tttaagttct tcaggaattt ttataatatc ataatgtata
    15001 ttctcattat cagtaacaga tacgatcata tttcctaaaa tagcaggagc aacattatct
    15061 gggtgacctt ctatttctga agctatattt aataattgat ttttatctaa attagcacct
    15121 gatagagcat ttgcagctat aactccagct actatacaag aggcactact gccaagacct
    15181 ctacatatag gtacttcact gtggattttt attttgatac cagcaggtat tttttctggt
    15241 ttaactctat caaaaaagta cttcatagat gtataaacta agttattttc gtttttgtaa
    15301 gcatcttcac aaccttctat ttcaagacca ctttcaattt cttcaacata aaatttattg
    15361 tatatattta acgcaattcc aagacaatca aatcctggac ctatattagc acttgttgca
    15421 ggaactataa tctctaacat aataatcacc tataccttta agaatttttt tatagctagt
    15481 tttaattcat ctttttcata aatatcttta tgtagtattt ctgcattttt aagattttta
    15541 ataggttttg gaatatctat tttagaaata cttgataatc tatcaattat agcaaagtca
    15601 tcaaggtctt taaaatcaca atctatagac tttagaacat cttcagaaaa cttgaatgga
    15661 cttgcagtac ttacaataac agtttttgtg ttatcagaag tttctttttt atatttttca
    15721 taacaattgt atgcaacagc agtatgagta tctatcaaat aattataatt tttaaacaca
    15781 ttgtttatcg tgcttttaac ttcttcttca tttgaatatt ctccatagaa tgaggctaaa
    15841 tttttttcca tatcatcact tattttgtag actcctttat cactaaggtc tgataacagc
    15901 ttatttacaa tatcagtatt tctatttgat atttcaaaca gtaatcgttc taaattactt
    15961 gaaattaata tatccataga aggagaagta gttaatttca atgttctatt tttatcatat
    16021 acaccagttt taaagaaatc ataaagaaca ttattatcat ttgatgcaca tatcaatttg
    16081 tttattggaa gacccatttg tttggcataa tatccagcta gaatattacc aaaattacca
    16141 gttgggacaa caaaatttat tttatcattt aaatttatct cactattgct aactaatttc
    16201 atatatgaat aaaaatagta tgcaacttgt ggaagaagtc ttcctatatt aattgaattt
    16261 gcagaagata atatataatt attatccaat agtttttgat taaatttttt atcagaaaaa
    16321 atcttcttta cacctgattg tgcatcatca aaatttcctt ttataccaac tacatatgta
    16381 ttttttccag tttgagtttt catttgaagt ttttgtactg ggcttacacc atcttctggg
    16441 aagaatacaa ttatctttat tttatctata tctttaaatc cctcaagtgc agctttgcct
    16501 gtatctccag atgtagcagt aagaatcaca acatctttct ctaaattatc tttctttata
    16561 gatgttttaa gaagatgagg cattatagta agtgccatat ctttaaatgc taaagttgga
    16621 ccgtgatata gttctaagaa ataagtatca tttactttat ttaatggagc tatactacta
    16681 caatcaaact tttcatcata tgcattttct atacaatttt taatttcatc ttcagtaaaa
    16741 tcacataaaa atttactaag tacaaaaaat gctatctgag aatacgttaa atttactaaa
    16801 tttataagtt cattttttac attgggaaat tcacttggaa cataaagacc accatcactt
    16861 gatattcctt ttattattgc ttgtgatgca gttacttttt cttcggtgcc tctcgtactt
    16921 gaataataca ttttttataa tcctccatat attttaaatt tttaattagc taattaaatt
    16981 agatacagaa tattttaaaa accgaattta ggtaataaaa aaagaccctc atccctaaaa
    17041 aagggacgag aatctgtttc ccgcggttcc acccaaattg attttaaaaa atccacttga
    17101 tactttaacg caagtaataa aaacgtctat tcctactaaa atcattaaga taacttcgga
    17161 tagaagctcc taggtagttt tcaatccttc taattaggga aacatttcag ctagtagttt
    17221 ccttctctgg taatcgattc agatttactc ttcctaatca atgcctttca atattcttaa
    17281 tattattaat tattctagtt tataaaacag acaaagtcaa gtattaatat aaaaatttgg
    17341 aaaaaatatt ttttaaattg taaaaaaatt gtaatcaaat tgagtattag aaaaatattt
    17401 ttttaacttt tttaaaagtt taaaaaccta gattttcata gtgatgaagc tgtttttaat
    17461 gaaagtaatc aaaagataac tattaaatat ctgtaatatt aca
//
LOCUS       DAELXX010000086        17138 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.086, whole genome shotgun sequence.
ACCESSION   DAELXX010000086 DAELXX010000000
VERSION     DAELXX010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17138
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.086"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(214..738)
                     /locus_tag="KPH29_002692"
     CDS             complement(214..738)
                     /locus_tag="KPH29_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG1468276.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQIMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(803..1555)
                     /locus_tag="KPH29_002693"
     CDS             complement(803..1555)
                     /locus_tag="KPH29_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBG1468277.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDISKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1586..2884)
                     /locus_tag="KPH29_002694"
     CDS             complement(1586..2884)
                     /locus_tag="KPH29_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HBG1468278.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEDIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMEDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQQGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREVNVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2901..5165)
                     /locus_tag="KPH29_002695"
     CDS             complement(2901..5165)
                     /locus_tag="KPH29_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HBG1468279.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5420..6238)
                     /locus_tag="KPH29_002696"
     CDS             complement(5420..6238)
                     /locus_tag="KPH29_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBG1468280.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGIDEKIIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIIL
                     LDEPTAGLDPVSTRSIVDIIKGLNKDNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFVNENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6231..6929)
                     /gene="cbiQ"
                     /locus_tag="KPH29_002697"
     CDS             complement(6231..6929)
                     /gene="cbiQ"
                     /locus_tag="KPH29_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HBG1468281.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIKNIFVLSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEIFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKMHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6932..7216)
                     /locus_tag="KPH29_002698"
     CDS             complement(6932..7216)
                     /locus_tag="KPH29_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG1468282.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPDYEPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGVQKGKRS"
     gene            complement(7206..7958)
                     /locus_tag="KPH29_002699"
     CDS             complement(7206..7958)
                     /locus_tag="KPH29_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HBG1468283.1"
                     /translation="MKQNIKLGVIAALMLIVLTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLVGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIISFLIFKALKKKDG
                     NNAMPVFLAAAIGDLATYTVTSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8147..8339)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8471..10243)
                     /locus_tag="KPH29_002700"
     CDS             complement(8471..10243)
                     /locus_tag="KPH29_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HBG1468284.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYNMDKHPALDSMTRRIVNDIRNIDTN
                     QDMMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(10257..11186)
                     /locus_tag="KPH29_002701"
     CDS             complement(10257..11186)
                     /locus_tag="KPH29_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG1468285.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            11415..11603
                     /locus_tag="KPH29_002702"
     CDS             11415..11603
                     /locus_tag="KPH29_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1468286.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRAKRRKEVQYE"
     gene            11596..12297
                     /locus_tag="KPH29_002703"
     CDS             11596..12297
                     /locus_tag="KPH29_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1468287.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLLDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSINNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            12299..13222
                     /locus_tag="KPH29_002704"
     CDS             12299..13222
                     /locus_tag="KPH29_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1468288.1"
                     /translation="MNTSVYLLLILALSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            13379..14302
                     /locus_tag="KPH29_002705"
     CDS             13379..14302
                     /locus_tag="KPH29_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1468289.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGCEKRVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDHTLRLYNTTLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            14295..15032
                     /locus_tag="KPH29_002706"
     CDS             14295..15032
                     /locus_tag="KPH29_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1468290.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPTKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFASIALGRRK"
     gene            15043..15816
                     /locus_tag="KPH29_002707"
     CDS             15043..15816
                     /locus_tag="KPH29_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1468291.1"
                     /translation="MWKLLWAECQKIRRSKIVWITFFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETKLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            15832..15984
                     /locus_tag="KPH29_002708"
     CDS             15832..15984
                     /locus_tag="KPH29_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468292.1"
                     /translation="MKKIKKLIILIVFLFLFGMLHCYTEWKFNNDPPKGVIERINEWT
                     DKIGLD"
     gene            complement(16357..16965)
                     /locus_tag="KPH29_002709"
     CDS             complement(16357..16965)
                     /locus_tag="KPH29_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBG1468293.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         5524 a         2979 c         2150 g         6485 t
ORIGIN      
        1 aaataaatat ataaacaatc ataataaaat ttttttaata aaactttatt ttttagttaa
       61 aatcatacaa tatatagctt tccacaatac aaataaatta aaaagtgaaa tgtatttgtc
      121 taaaaacata ataacatttc actttatcct catttttaag taatatatat ttttaatttt
      181 tatttataac tcaatctagt ttttatataa gttttagttt ttttctacat ctgagtaatc
      241 tattccaaaa gtttctacag taacagactt cataatttgt ggagtttgtg gcttatcagc
      301 tgaatctgtt ctaactgatg ctatttcatc aactgtatcc ataccttcta tcactctacc
      361 aaaacctgca tactgaccat ctaagtgtgg tgagttttta tgcattatga aaaattgaga
      421 accagctgaa ttaggtgcca tagttctagc cattgatatg actcctcttt catgttttaa
      481 attgttagta aatccattac cactaaattc accttttatt gagtgacctg ggccacccat
      541 tcctgtacca tttgggcatc ctccttgtat cataaatcct ggtattactc tatgaaatat
      601 tattccattg taatatcctt catttactaa tgatacaaag tttttaactg tatttggagc
      661 tatatttgga tataactcaa ttttgatttc ttttccattt tccatttcta tagttactat
      721 aggattttta ttttccataa tttattacct tccttttcat tagttaaatt acataattac
      781 atttattata ttatactcta aattaaagtc tatcaagtat attttttcct atttcgtatc
      841 cttcttgata aatagtttta caaaactcat catcaaagtt aagcgtatct ttaggagcaa
      901 attgagtatt aggtctaact actattattt tatcttcatc atatactttt ttaatatcct
      961 ctggcaactc ataattatat tttgtggata ttaaaataat tttatccaca ttatccccaa
     1021 ttaatggttc aattaatgca ctcctaatta aacctccatc taatttgaaa ccatcataaa
     1081 ttccttcttg cacatctttc acaaattgtt ctttaaatcc aagagttgcg tttggatttg
     1141 atggtaacaa cccactgtat gaaatgctga taatggcttc attacgaggc atttttgata
     1201 tgtcaacaat cttttctgat actactttat tatcaacttc aacataatta acataaaagt
     1261 tcatttcttc tatattacta tcatctagtt cccttaggtt gtctatagca ggagaattat
     1321 ccacagtatt atttactatt tttacatttc cattctggat atcttttata tttgtccaca
     1381 ttttctccaa gttatcaaca ttccctgtgt acaaataata tccatttatt gctcctattg
     1441 atgtccctga aatcgaataa aatttattta ttcctccatc atacaaggct ttaactactc
     1501 ctgcttgata agcccctttt gctcctccac cttctaaaca taatcctaat ttcatagtct
     1561 catctccaaa ttgtttatct tatatttata tttcaaaaaa cttactaaat gttttatata
     1621 tactatagtg gtcctcaaca tttcctaatt ctcttataga gtgcattgct aaaattggac
     1681 ttcccacatc cacagatggt atatctatat gagtagaaga tattggacct atagtacttc
     1741 ctcctctttc atctgaccta ttaacaaatt cttgatactc aacattaact tctctacata
     1801 tatttttata aatagctata gaatatccat cactagtata tgcctgacta gcatttatct
     1861 ttataactgg accttttccc attattggtt tattagttgg gtcatgcttt tctggaatgt
     1921 ttggatgaat agcatgagct aaatctgatg atatcataaa agatgaataa atagactcaa
     1981 agaatccctc tctatcctta cccattgata tgcaaattct ctccaatata tttaacaaca
     2041 tgtttgaatc tgcaccttgt ttagtagagc taccaacttc ttcattgtca aatacagcca
     2101 ttacatttac accttgttga cctttagtat taacaagagc attcaaacta gcatgtacca
     2161 ttgcaagatt atcttgtctc ccaatagata taaattcatc attagcccct attaaacttc
     2221 ctttttcaaa ttcatataaa aataaatcaa aatctataat atcttccata tttacatcta
     2281 agttagatgc tatttgcttt ataagaaaat tatctttttc taatgtatca tttaacatcc
     2341 caactaaagg taacatatct ttttgcttat tatatgaatg accatcattt atacttctat
     2401 ttaaatgtat tgctagattt ggtattatac aaattggctt tttaaagtct ataagccttt
     2461 catttggttt taatatacta tcacctttta aaactactct accagctatc ccaagaggtc
     2521 tatccaacca tgtacttaat attgctcctc catagcattc tgtatttaac ttcaaataag
     2581 atttttcagc ttctatttct gcatttggtt taatactaaa tgtaggagaa tctgaatgag
     2641 agcctattat tctaaatcca tctctttcta tatcttctga atttactaca aaagctacta
     2701 tagctgatag attcttagta acataatact ttccaccaac tcttagattc catctttgat
     2761 tcaaaactaa ttcttcaaat ccatttttat ctaagatttc cttagtactc tcgactgcat
     2821 gaaaagacga cggactatca tatatgaagt ctattaagtt tttagcaaag tttatactat
     2881 ccattcctca tttacctcca ttataaaata tttagtgttg tatattcttt atttattttt
     2941 ttaattccct gttggtcaaa agctatagta acatctgtac caattataga aaccactgtc
     3001 ccaaccccaa atttaggatg atgtacctta gaaccaagtt ttatatcatc tggattagtt
     3061 tctttattag aatcttttat tgtagcatta actttatctg caactttaga tttgcttata
     3121 gtactcatat actttttagt atatttatct aacatattat aacttgcttt agaatagctt
     3181 agttcttttt ttactttgta caatctttct atacattctt ctggcagttc ttccataaat
     3241 cttgaagcta ttgcaacatt tgttttacca tataatgttc tcttctctgt caatgacata
     3301 tatagctctt ctttagctct agttatacca acataacaaa gtcttctctc ttcttctatt
     3361 tgtgaatcac tcatagattt tatagctctt gatataggaa ataacccttc ttccatacct
     3421 gttaaaaaca caacagggaa ctctaaacct ttggatgtat gaattgtcat aagcgaaacc
     3481 ctatcattgt cttcttcatc atttgattca gatgtaagtg ctatacttgt taggaatgtc
     3541 tctaagtctt tattttcata agtatcatca ttactacttt caaactccaa agctatagaa
     3601 ataaattctc tcaaattatc aattctgtct tgagcttctt cacctttgcc tgttaaatct
     3661 ttttcatttt tatttcttat ctctacaagc tcgtccatat atcctgtagt atccaagact
     3721 ttttctataa gtttgctaac aggataaact tcttttatag ttctaagagt ccctataata
     3781 tcaacaaatc cacttatact tgcccttgcc ttagtagata tatctgaatt tgtttctata
     3841 tcaattaata ctgagtatat actttcctgt tttaagttag ctctatcttc aattttttct
     3901 atagttctaa gccctatacc tcttctagga acatttataa tcctttttat agatatatca
     3961 tcttgtggat tttgaataac tcttaaatac gccaacaaat cctttatttc ttttctctcg
     4021 tagaattttg taccaccata tatattgtat ggaatttgac ttctatttaa agcgtcctct
     4081 acaggtcttg cctgtgcatt tgctctataa agtactgcaa aatccttata aggtctattt
     4141 tcttttctag ctatttgtgc aattgaatca gcaatgaaat cagcctcttc tatttcgcta
     4201 ccagttaatt gtatctttat aagttctcct tctttttttt cactccaaag cttttttctt
     4261 tttctttcta tattattaga tataacttta ttagctgcat ctaatatcac ttgtgtagac
     4321 ctataatttt gttctaactt tacaacatgt acattgtcat aatctttctc aaattcaagt
     4381 atgtttctta tatctgcgcc tctccatcca taaatacttt ggtcatcatc tccaacaaca
     4441 caaatatttt gatgttctct tgctaataat tttatcaatt catattgagc tttacttgta
     4501 tcttggtact catctaccat tatgtatcta aatctacttc tgtaatatgc taatacttca
     4561 tcattagcct tgaaaagttc tacagtttta agtattaaat catcaaagtc aagtgcacta
     4621 tttcttttta aacggtcttg atataacgca taaatatctg caatcttaac cattctatta
     4681 tctgccatgt tgatatcttt aaactgcttt ggagtataaa gtttgtcttt tgctcctgaa
     4741 attgcactta taactgcttt tggttcaaac actttatcac ttaaatttaa ttcttttaga
     4801 cagtctttaa ccaatgtaac ttggtctgct gaatcataaa ttacaaaact tctattgtaa
     4861 ccaatcttat ttatatcttt tcttagtata cgaacacagc aagaatggaa tgtacttatc
     4921 cacatatcct tagtttcagg acctatattt tgttcaactc tctctctcat ttcatttgca
     4981 gctttgtttg taaatgttat agctaatata tttggtgctt gtacaccttt atcttctata
     5041 agatatgcta ttcttgttgt taatacctta gttttacctg aaccagcacc agccagtata
     5101 agtactggac cttcagtctt ttctactgct tctctttgag caggatttaa tgtatctaaa
     5161 ttcatatttc tcctcctgtt aacagccata aatatatttt aatctaaaat atgaattact
     5221 tcaaattata attatgtatt atattaatag attatttcca atatattaca attaaataac
     5281 atttttccat ataatttatt taattgttta ataaaaaaaa taaccttatg atttggggaa
     5341 tcataaggca cctttaaggg gtctaggtta agggttatta tatataaatc tattttttta
     5401 aaataggttt agggcttaat tatttatttg tattaagttc tctttccttc caatacttat
     5461 acaaatcttc ttctttctta aataagggta aattcatatt tctatgtact tttactaacc
     5521 atggggattc taggccagct tgtataatgt tattttcatt tacaaataca ttttctgcct
     5581 ttccttcatc aattaaaatc cccttatcca atacatatat ataatcgcat atttcataca
     5641 ttaaattcat atcatgactg gatattacta tttttatatt atctttattc aatcccttta
     5701 ttatatctac aatagaccta gtacttactg ggtctaaacc agctgtaggc tcatcaagca
     5761 atataatttc attttccatt gctataactg atgctatcgc tacccttttt ttctgaccat
     5821 aacttaaaaa atgtactggt ctatctataa aatctattcc attaacagct actagagctt
     5881 tgtttattct ctctttaatt attttttcat ctataccaat atttctcatt gcaaaggcta
     5941 tatcatcata tacttttgaa taaaaaattt gtttttctgg gtcttggaac acaataccta
     6001 cattttttct aaggttgtat aaacttctct tatcatattt taacttttct tctttaaaaa
     6061 gaatttctcc agatgtaggc tttaatatac ccattaaatt cataaataat gttgattttc
     6121 ctgaaccatt tgaacctatt atacctataa catttccctt tgaaaaatcc atattaatat
     6181 ttaatagtgc attggtattc ttttcatatt gataagttaa attatttatt ttaaacatca
     6241 ttatccccta ctatgtgaaa tttaccatca tacaatttta tgtctaatga tatacacata
     6301 tcatcatacc ttgtcataag tcttttgtac agcatactgc caagaattcc taatgattta
     6361 tatgaagttt ttaaatttat gtacccaaat cttagttctt gtgacttata tatttctgaa
     6421 aattcttcta aaaatatgaa tataaaccta tatattaaca ttgatagctc aacaaaagta
     6481 tcaggcaaat gcattttttt aaagaagaat attaattgat taaaaggtgt tgtaagtata
     6541 caaaagtata cacaagtcaa acaagacata gctctaaata acacatgaat agacatgtca
     6601 atagatgctt ttgatattcc tacattgaaa ctaaaaattt caaaactata taataagtct
     6661 gccttattaa aagatatatt tacaagagtt attcctatac taagaaataa aaagtacatt
     6721 ggtattctga gtaattttaa ataattcttt aaatctattc cagctacaca aacaactaga
     6781 atactcatta aaatcataat tccacttaaa acaaatatat tttttattac catagaagca
     6841 atcagaaaga ttactcctat ggctactttt ttatttggat ttactttact taatctattt
     6901 gtatatgcat atttatcaat tataagcata attaacttct ctttcctttt tgaacaccta
     6961 atatgtaccc tattacacca gcacctaacg ctgcttgtgc agagaataat aaactttcta
     7021 tttctccact tggtggctca tatgggctac taaaccatgg ttcataatct ggatttatct
     7081 tagtaatttc actctcagct tgaccatctg ctcctccata ttcagctccc gaattaacaa
     7141 gtaatgggaa tatgataaga gctactacta ataaaagtag taatatatta gttttcgtct
     7201 ttgttttagc actcattagc tttaactcct ttatcattat tttctagtat gcttttttct
     7261 ccattttctg taattaaatt atatacaatt acagtaagaa taccctctgc tattgctatt
     7321 ggtatttgag tcataaagaa aattcctaaa aacttaattg ctgaagccat cactccacct
     7381 gatgggtctg ggaaagccag tgctaactgt atcgaagtta ctgtatatgt agccaaatct
     7441 cctattgctg ctgcaagaaa tactggcatt gcattatttc cgtctttttt ctttaatgct
     7501 ttaaatatta agaatgatat aattggtcct ataatagcca ttgaaaaagc attagctcct
     7561 aaagtagtta ttcctccatg tgccaatagt aatgcttgga atattagaac tatagttcca
     7621 agaacaaaca ttacacttgg tccaaacatt attgctccaa gacctactcc agtaggatgt
     7681 gaacaacttc ctgttactga aggtatcttt agtgctgaca atacaaatac aaatgctcca
     7741 cacagcgcta ataatacttt ctttttaggg tcttgcttta ctatttttcc tattgatttt
     7801 agtcctacta aaaaaaatgg tataaatata actccccatg ctatagacca ttttactggt
     7861 aagtatcctt ccattatgtg cattgcattc gatgtaactg gagttaaaac gattaacata
     7921 agtgcagcta taacccctaa tttgatattt tgtttcattt tactatcccc tctctccctt
     7981 tctaataatt aaaatataaa taatatatgt ctgatttatt ttaaaaataa aaataaaaaa
     8041 gcctaactcg atggctaggc ttctaatttt cgataatcca aatacaaaca cataaaatga
     8101 acatatctgt ataattatta taagaaactt tctccatccc cgaagaaatt actttaatat
     8161 tttaggcagg tctcctgact tagcttcttc ctactcctct tccttcccat gagatataaa
     8221 aaatatcatc atagtggcta acaaaagttt cgtctgcatc acagtagcgg gggctgtaaa
     8281 ggaatttaac ctttttccct attaatcttg cttaaagaac ctataatatt atatatttaa
     8341 tttttaaatt attcttaaat cattttctat ataaatcatt gtataccttt tctgtgaaaa
     8401 tttctacatt ttttataaat tttattcatt tttatatttt tatgtatata aattcttttt
     8461 tgtaagttta ttaatttcta attatcttct ttaaatacat tgaaactaca tcagaaaatc
     8521 tagttatatc tcttgaatat ataaaatcat ttccatatat taatctttca gcttcaagtt
     8581 ctgattcttt tcctgtaaat actcctagta ctaatatatt ttgctttcta gcttttctca
     8641 cttcattggc tgtatctctc aaaccaacta tacccctata agccttttct cctcttaatg
     8701 ttctctcact actttttcct atctttacat catttggtct tccatcgctg agaactatca
     8761 ggattttatt ttcttcatct ctatttatta gaccactaca tatagatttt attgccaaac
     8821 catctctatt attaccagca caaaaatatt caaatatatt ttcactattt tttatactat
     8881 catcataatc tttaaatctt tttaatattg tatagtctaa aaaactagaa aatcccatca
     8941 ctctatttgg tatgccaact tctgttagag catttgcaat tatgtaagct tgaattgcca
     9001 catttccctg atttctactt tgagagccac ttgcatccaa taatatatca ataacgtatt
     9061 tccctttttc attttctata tctttataaa atattttatt attattactt ctaccaactc
     9121 tccaagctct atttgctaat atagtaccac aatcagaata tactctactt ttagcatttt
     9181 cttctattaa aattcttgac agactttctt ttaatctctt tatttgctgt ctatacattc
     9241 tagcatgttc tctaaatttt cctaaattat tttctttttg tcttgttaca tattttagtt
     9301 gaaatacatt attacactca cttctaagaa ctccatctgt aaagtgtatt ctacaacctt
     9361 catgaacatt tctacaattt tgtgtctcaa tttttttaat ttcaccttta ctcaaataag
     9421 tttttccata ataatgttct atttttgaat atatcttatt tgcagtgtct tcatcaacat
     9481 atataactcg gtctgctgaa ttgctacatt tagaatcatc aaaatcccct acaccttcta
     9541 caagcatgga tgaagatatt gaatctatat cactttcaat aatattttct tcttcatcac
     9601 tataaagttc ttcatacatg aaatcagcaa atgtatcaaa atttacttct acactttcta
     9661 cattattttc atcaataaga ctattttctt ctccatttaa tatgtattcg aaataagtta
     9721 aatatataac atctaatgct tttaaaatct ccatcatatc ttgattggta tctatatttc
     9781 ttatatcatt aactattctt ctagtcatac tatctaatgc tgggtgcttg tccatattat
     9841 aaagtacaaa tgtgtattta attttatcca taatattatc attgtgtatt ttggtaaaac
     9901 gtgaaacaat atctttataa gcatttcttc ttatgtcttc tatccctggc ctatctttaa
     9961 tgaccttttc ttcaactatt gtatttaata taacttgaat tagacccagt ataatatctt
    10021 tgttgtatcc ttttcttatt ctatttgtaa gatatttttt aactatatcc caatctacat
    10081 actttcttct agcacctgca attattgcaa aatatagacc tatttctttt gaagagtaat
    10141 ctttttctga tatatctata ctttcattgt aatcaccagc tattgtccat gataagttat
    10201 tagctctatt ttcaaattca taatcatcat atatccattt cataatctca cctatattaa
    10261 atatgactta ttggaaatac atctatactc tcccatttat taggtattct agttttaatt
    10321 acatctccaa ccatttcttt ctcatataca tcaaaagtct ttcctgtaag tcccatatta
    10381 attgcaagag ttggttttaa tcctcttctt atagtcttaa gagatgccat aagaccacgt
    10441 aaatcaatag ctttactaga tatctctcca ttttgagact tcaattgtaa atctaaaaat
    10501 attcctgcaa agtgtattaa tttttcttca tcagcatctg aaaattcatt ttttaaaatc
    10561 atcattaact tatcttcttc tataggagga atatctatta ccataaatct agataccagt
    10621 gcttcattaa gttcttttgt tcctgcatat tcataattca tagtcccaat aaatcttgtt
    10681 gctggatgta gattgactct ctcataccca ggtacatcaa ttattcttct atagtctaaa
    10741 gctgaatgta ccacaactat agcatcattt tttgccatat taatttcatc aaatactcca
    10801 aaacctccat taatagcaca ctcataaact gagcctcttc taagtttaac ttcattgtct
    10861 ataaatgtat ctgtacctat aagtgttgaa ctgtctgtat ttatatgaaa tgatgtattc
    10921 cactgaggtc tattaaatat ttcccctaga ttatcagcaa gtaaattttt tcccgttgcc
    10981 tttggtcccg ataataatat attttcttct tctaaaatag ctgctataca catagacaag
    11041 atatctttac cataaaaata agttttagac tttgttactc tgtattctaa atctttttct
    11101 aaaccataat aatttctaaa atacagtaca tcttcaataa gtttttcatc taccccttgc
    11161 acccttagat tatctattat atccatattt ttaccccttt tatataaata gtatttgttt
    11221 catttcttat caccatttta gcataaaagg atataagcta aaattgattt tttaatctcc
    11281 cccattttaa gtaaaaccat gcaccatttt atcactacaa aattaagagt actacttact
    11341 gatatttctt gattttgttc gtctatacgt atataatata tgctgataaa ttatgaggaa
    11401 aggatgattg aaatatggaa tatatgtctt gcccagaagt agcaaaaaaa tggggcattt
    11461 ctgaaagaag agtacaaaaa ctatgtgaag aaagtcgcat accaggagtt tcaaaactcg
    11521 ggtatatgtg gctgatacca aagaacgcag aaaagcctgt tgatggtaga gcgaaaagaa
    11581 gaaaggaagt acaatatgaa tagcgttttg attatagatg atgacaaaga actttgtgct
    11641 cttatgaaaa aatgtgttga gcaagaaaat ttatctgcta ttactgcaca ttgtggtgca
    11701 gaaggtctac atttgctcga taaaaataga cacacactct ctcttgtaat tttagacata
    11761 atgatgccag atatagacgg ttttcaagtt ctgaaaaaaa ttagagaaac aagtaatatt
    11821 ccagtaatta tgcttactgc taaaaatgac gaggatgata aagtttccgg actacgttta
    11881 ggtgctgacg attacctaac aaaaccgttc agcataaacg aacttatggc tcgtgtcaat
    11941 tctcttattc gtcggtatac tatgttaaac cctgctttta taacagacac agattgcata
    12001 ataataaatg gcatgattat tgacaagaca aaccgaattg tttctatcaa taatattcct
    12061 gttcaactta caagcaaaga atttgattta cttgcttttc tggcttctaa taagggacgg
    12121 gtatttacca aaaaacagat ttatacacaa gtatgggaag aagaatatgc ttttgacgat
    12181 agtaacatca tgtctttcat cagtaaattg agaaaaaaga ttgaaccaga ccccaaccat
    12241 ccattttata ttttgacagt tcgtggtgtc ggttatcgtt ttaataagga ggcttaacat
    12301 gaatacaagt gtctatctac ttttgatatt ggctcttagc ctgattatta tagcgtacct
    12361 tgtttctgtc ctgctacgtg tccgaataca acttacactt ataaaagatg ctttagagga
    12421 tataaaaaat ggaaacctta atcgccgtat tttaacagat gaaaacgaca ttacaaaaca
    12481 aatttgctat gatattaacg aaatagcaat caatagccaa tttcaactta ttcagcaaaa
    12541 acaatcagaa caagcctaca aacagctaat gacaagtctc tcccatgatg taaaaactcc
    12601 acttgcttct ttggtcggtt atttggaagc agtagaaagt aaaattgttg taggagagga
    12661 aaaagacgag tatatccatg ttgcgtctaa taaggctcat tacttaaaga attttgtaga
    12721 aaatctattt gaatgggtaa aattggattc taaggaacaa gtttttcatt ttgatatttt
    12781 tgacttgaat gaattatccc gtaatattat atctgattgg atacctgttt tagaaagcag
    12841 tcattttgaa tatgaatttg atataccaga aatagagtac ttcttacgta tagacgcaaa
    12901 tgcatatacc cgtatcatta ataacctatt acaaaatatt attacccata gtagtggcaa
    12961 caaaatgaca ttgagaattt ttgaaaacaa agagcaagct caaattataa taacagataa
    13021 tgggaaaggt atatcttcag acaatctccc acatattttt gaaaggttgt accaatgcga
    13081 ccattcacga gcttcaaagg gaaatggttt aggcttggcg attgcaaagg aacttattaa
    13141 cgctcataag ggaaatatta ccgccaacag cactcccggt atgggaacag aatttacaat
    13201 attgcttcct aaagccctgt aaaagtacga ggctttctta ctctaaaaca agataaaagc
    13261 aagattatgg caaggttttg gcaaggttca tgttttataa ttctaattgt agttgatttt
    13321 gacgttgtat caactatctt ttgtagaaac aaatttatta aaagtgagga aattatatat
    13381 gaatgatttt gtgattgaaa caaagcaact tacaaaaatt tatgggaatc aaacagtagt
    13441 tgacaaagtc aatctccatg taaaaaaagg ccgaatatat ggtctgttag gacgcaacgg
    13501 agctgggaaa accacaatta tgaaaatgat tttaggattg acttcaatca cgtatggcga
    13561 ggtagatgta tttggagaga atattaaggg atgtgaaaaa cgagtatatc cccgtatcgg
    13621 tgcaattatc gaaacgccag gattttatcc taatctgaca ggaacggaga acttggagat
    13681 ttttgcaaaa cttcgtggta cagccgcccc taattctgta aaaagagcat tggagattgt
    13741 aggcttacct tataaaaata aaaagctgtt tagtaaatat tcacttggca tgaaacagcg
    13801 acttggtatt gccaacgcta tcctgcatga cccggaactg ttagttttag atgagccaac
    13861 caatggactt gaccctattg gtattgcgga gatgagaaag tttattaggg acttgagcgt
    13921 tgagcatgga aagacaattc ttatctccag tcatattctt tcagaaatct cattgttggc
    13981 tgatgatatt ggaattatcg accacggtgt tcttttggaa gaaagtagta tgagtgaact
    14041 agaaaagaaa aacagtaaat atatcctgct acaagtttca gatattccaa agacatcact
    14101 tattttagag cgtcaattta acacgaagga ttattccgtt caagatgacc acactttacg
    14161 cctttataac actactctgg atatgggtga aatcaataaa gcacttgtaa tgcagaatgt
    14221 aactgttatt agttctcaac tttgtaatga tactttagag gactacttta aaaaaatcac
    14281 aggaggagag ggcattgctt aaacttattc aaacagaatt tcttaaactg cgtcgtagga
    14341 aacttatttg gctgatgttg ttgtctgctc tcattatgcc gtttttcgca ctactatact
    14401 tcaattattt tggtaaaaca ggtgttgagc caacaaagtt ttataaatgg tctgcatttg
    14461 gttatacact ttggattatt ttgcccgttg ttttgggtat attttgcacc atgcttatac
    14521 acgaagaaaa ccagtatgat atgctcaaac agctttggat tgtacctatt agtaaaatgg
    14581 gatacttttt tagtaaattt ttcgttgttt tgatttactc tatttgtttt atgctgatta
    14641 cagccgttgc ttctgtgtta tttagtgtac tttctggata tgtcgtattt agctgggcaa
    14701 gcattttata tttgttgaaa aaatgtttag aaattggtgt aatcactgct tttgtaatgt
    14761 tgccgatttt ggcaatcgct acatctcaaa aaggatacat tctcccaatt tgtatcacat
    14821 tggtatatgc cttttccggt tttgttataa tgacaataaa tatgtattta cacccattat
    14881 ccagtatggg agttattatc atgagaaata aagacattcc aggtcttata ttttcacagg
    14941 aaattaatgt cccattagca tttctttgta tttgtatttg gagtattgta tcagtattgt
    15001 ttgcaagcat tgcactagga agaagaaagt gaggtgtaga taatgtggaa attactttgg
    15061 gctgaatgtc aaaaaattcg tcgttcaaaa attgtttgga ttacattttt tgcatccatt
    15121 atgatagcag tcatagtttt tgcaggaggg caggatgtat ataatgaccc agatttacat
    15181 tatggcttaa agagcgttta tgacggttca cggtatatag acaatgcagg ttggtatatg
    15241 gacgcggtac aaccttggtc aacttttttt gttcttcctg ctgtcattgc cctattagga
    15301 agctatatta tttgtcgtga agaagaggag gacactatta aatccctgcg tcttattccc
    15361 ataaatgaaa caaaattaac catagcaaaa atgattatta cttttatatt cagtatactt
    15421 ctttatttgt tactctttac tataacattt ttgactgaat ccgttttaca tttttccgat
    15481 ttatcgacaa aactggtttt aagttgcatg aaagagtatt ttcttgatgg cataggtgta
    15541 ttctttgcaa tttctcccat tattgccttg gtatcgcgca tgaaaaaagg atattggctt
    15601 gcattagtat tcacagaagt ttattctatt gctggactat ttgcgggtat gtcaaatgtc
    15661 ttacaaacct tttttcctat tgcagcaata tttaatattt ctggatacta tataactact
    15721 ggagaaaaaa ttatggggag tatcataagt ttattacttt gtgcctgcct ttctgcattt
    15781 atactgaaag ggctaaaaca caatgaaaaa aattgaaata ggtatatcaa aatgaaaaag
    15841 ataaaaaaac tgattattct aattgtattc ttgtttcttt ttggcatgtt acactgttat
    15901 actgaatgga aattcaacaa tgacccacct aaaggggtaa tagaaagaat aaatgagtgg
    15961 actgataaga ttggactgga ttagtaactt atgttaatta tagtgaatgt aatattagca
    16021 agttattgtt tcgtatttta atactataac tttaaatatt tggttttaat cttccgaacg
    16081 aatataaaaa aactgtcaca gaggcaacat atttttgaat atttataaag tggtagtttt
    16141 gattttcaaa gctgccgcct tttcttctat ataagcaaac ttatcctcaa caaagtgtaa
    16201 aacatggtac attttaagaa aaaagtacca cagacacaag caataaaaga aacgccgaga
    16261 acctacaaaa acgtagtgct tatgcagatt tacgacgcct tctattttct caaccgtctt
    16321 aatgaccttg tacaaaagaa atttttctaa tattttttat aatcttatct tgtgatgttt
    16381 tttatgtata aatgacttaa taacaaatcc aataaaaatt atggatattg gtataattag
    16441 aacgaggaat ttataatcat tataatgtga cattattctt acccaactct cagaaaaaat
    16501 atatcctata ctgattaatg aagtattcca tatggttatt cccacgatag atgatattac
    16561 aaaagttata atattttgac ctgctatacc tgctataaaa gatatgtatg ttctacataa
    16621 agggattaat ctacatacac aaacagaaat agaagagtat ttattaaaat aatcttgaga
    16681 tgcaaatatt cctttctttg cctttggaca tatctctatt attttattta tgatagattt
    16741 acctccccaa tttcccactg tataacaaaa tatcgaacca agtatacctg caattatact
    16801 taaaattaaa attgtcagaa atccaaaatg atttcttgca gcaattgctc cacataaagg
    16861 taaaacaact tcgctaggta atggaaaaca agcatactca atcataatta atacaaatac
    16921 agagattaat ccatattgta aaataaaact attgattaca gacacttact tatctatctc
    16981 ctttcttatt taaaggtttt aagggtacat attctaagga ttctcaccct atatttaaag
    17041 aatcaatctc tttaaactat tatatgtatt tacacaaagc atttataaca tactttaaaa
    17101 atagtacctt aaaataagaa acttaataaa taaaaaaa
//
LOCUS       DAELXX010000087        17085 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.087, whole genome shotgun sequence.
ACCESSION   DAELXX010000087 DAELXX010000000
VERSION     DAELXX010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17085
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.087"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            230..2089
                     /locus_tag="KPH29_002710"
     CDS             230..2089
                     /locus_tag="KPH29_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG1468294.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVC
                     GASQRLTKLEMNHQPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICINKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHSQKI
                     NCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHMLKE
                     LNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEFEEILDKEFVYNS"
     gene            complement(2381..2980)
                     /locus_tag="KPH29_002711"
     CDS             complement(2381..2980)
                     /locus_tag="KPH29_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG1468295.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(3083..3880)
                     /locus_tag="KPH29_002712"
     CDS             complement(3083..3880)
                     /locus_tag="KPH29_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG1468296.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(3993..4098)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4190..5176)
                     /locus_tag="KPH29_002713"
     CDS             complement(4190..5176)
                     /locus_tag="KPH29_002713"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBG1468297.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLNEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIRHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     gene            5660..7390
                     /locus_tag="KPH29_002714"
     CDS             5660..7390
                     /locus_tag="KPH29_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HBG1468298.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLAIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            7500..7580
                     /locus_tag="KPH29_002715"
     tRNA            7500..7580
                     /locus_tag="KPH29_002715"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7534..7536,aa:Leu,seq:tag)
     regulatory      8477..8581
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            8791..11265
                     /gene="gcvPA"
                     /locus_tag="KPH29_002716"
     CDS             8791..11265
                     /gene="gcvPA"
                     /locus_tag="KPH29_002716"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HBG1468299.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGAESEKFIQNLVTNDISTLKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAMNQTRKSKIVVSKTIHHETLSVLRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYMATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            11265..12722
                     /gene="gcvPB"
                     /locus_tag="KPH29_002717"
     CDS             11265..12722
                     /gene="gcvPB"
                     /locus_tag="KPH29_002717"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HBG1468300.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIEDMIDQDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINALKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPLIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
     gene            13145..15502
                     /locus_tag="KPH29_002718"
     CDS             13145..15502
                     /locus_tag="KPH29_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBG1468301.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKDDFLYSGSFVVSGEAFVRVENIGKDNYANKIVNDAKISKKHNSQLRNSLN
                     SILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKIRIEVEKYSKKGNRVIVLAHSDSYMEDNNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDNDIYEAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(15588..15905)
                     /locus_tag="KPH29_002719"
     CDS             complement(15588..15905)
                     /locus_tag="KPH29_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBG1468302.1"
                     /translation="MSEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
     gene            16244..16999
                     /locus_tag="KPH29_002720"
     CDS             16244..16999
                     /locus_tag="KPH29_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HBG1468303.1"
                     /translation="MAHICFEDICYLLLKEAMKLKLIPHNKIVLFEDDLSIGNIFDVN
                     NYELRENIFKIRNVFENNPKLLFKPDLLEDRKLSKCRNIIIWCANNPKDLCGVYYVVS
                     KFQDKNIKIALVDEKKTEDALIRYSFTSEMSPEDFPFFLDKTLEIGFNEKLEFKNKWD
                     VLVKENSIFRALVGNEIKSVEETYFDKYILRNISDKWQPLIEVVANCMFEDEMRVKDW
                     FILWRLEKMASLKIVKIKGSKGDFYSDKEIRKV"
BASE COUNT         6512 a         1935 c         2796 g         5842 t
ORIGIN      
        1 taattaaaaa gtatataaat tatattaata gtaataatct ataagaactt atatattaga
       61 aaaaaatttt tgtatatata aaggactcta aagatgaact atataaaaca aggtttatat
      121 attctaatag tatagaaaat tttattgtaa acgcatgtat attattagaa aaaatagttt
      181 gaaggtattc aataaacata aaaaacaata taggggataa aggtgtttta tgaagagatt
      241 tttgaggaga attatcttag ttttatttat tttgttgatt tttataagtt ttatttctat
      301 taaattaatt cataatgttg gagattatgg aaaattgatt aattatgttg ggattgtttg
      361 tggagctagc caacgtctaa ctaaactaga gatgaatcat caaccaaatg atgaattaat
      421 agaatatatt gatgaaatcc tacaggagtt gattacaggg catggagatt atggtttagt
      481 aatcactgat tgtaatgaat acaatgagga cttactttta ttagaaaaaa agtgggaaga
      541 tttaaatatt gaaattaaaa aagtaagaat gaaagaacag aataaccagt tattaagtat
      601 aagtgaagag tttttttctc ttgcaaatga tacagtgttt aaaatagaaa acttttctaa
      661 agaaaaaagt aattacttaa tgactttaat aataattata tctattatag gtatattagc
      721 atgtatcata ttgattttac aatatagtaa aaaaatggtt agactggaaa aattaaatgt
      781 agatttaaaa aatattgcat ataaagatga actaacagga gtaaatacta tagaaaaatt
      841 taaattggat gcaaatcaaa atatttgtat aaataaggat aagaaatttg ctgtattcta
      901 tattgatttt gaaaacttta aatacattaa tgatatattt ggatatgatt atggtgacat
      961 gatactgaaa agatatgcta atcttatgat gaatgacata gggaaatatg aaatatttgc
     1021 aagagaaatt gcagatagat ttgttgcgtt aagatgctat gtagataagg aagatttagt
     1081 agtaagacaa cgagatgtag acaatgaact aattaatata acaaatgaaa ttaaaaataa
     1141 acatagtatt actgttgtca gtggaatttg ttgcattgaa gatgtaaatg aaaaattaag
     1201 tatagatggt ttaataaata gagcaaattt tgcacaaaaa actgttaaga ataaaccagg
     1261 tactaattat gctttttata atgacagtat tcgtaaaaaa atgattgaag aaaatactat
     1321 caagagtaga atacatgaag ctattgaaaa aagagaattt atagtttatt tacagcctaa
     1381 agttaattta catagtcaaa aaataaattg tgcagaagct ttagttcgtt ggcttactcc
     1441 ggataaaggt attatatctc cagcaatatt tattccagta cttgagaaga atttttttat
     1501 tgccttagta gataagtatg tttttgagga ggtttgtaaa tggattagaa agaggttaga
     1561 tgaaaataaa ccttttgttc agatatcagt taatgtttca agaatacaat tttataacac
     1621 taaatttgta gaaacttatt ccaatataca aaataaatac aggataccta aaaatactat
     1681 agaaattgaa tttacagaga gtgttgcttt tgagaatcaa aaacatcttt tggaaattat
     1741 acatgatttg catgaaaatg gatttacttg ttctttagat gattttggaa aaggttattc
     1801 gtctttaagt gttttaaaag atttaccatt tgatgtttta aagcttgact ctatgttttt
     1861 caaggctagt ttagataaag ataaagagaa aatagtaata aaaaatattg tacatatgtt
     1921 aaaagagctt aatatcacaa ctgttgctga gggcattgaa tgcaaggaac aagttgaatt
     1981 tcttagggat attgggtgtg atttagttca gggatttgta ttttataaac caatgccaat
     2041 attagaattt gaagaaatat tggataaaga gtttgtttat aatagctaaa atgtacttta
     2101 taaaatatga actaattaag taaaattatt ttgtactgaa taaattttta ctttatttac
     2161 agaatagtga ttatttacag aaaagggcga aaagtgtatt cctttaagta tgggatggat
     2221 agcacaaata ggggagaaaa aatattggcc atataagaat gcataataat ataaatattt
     2281 gaaaggtcag cattatataa aaattaaaaa gaaaataaat aatttttcta taaaaaagag
     2341 actatgagaa aagttactca tagtctccta aataatgtat ttattttctt cctgcaaccc
     2401 attttaagcc ccaatgaaat tcagtatcca tttctctatg acctttgaag tatttaacta
     2461 ctttttcaac actgaatgtc tcatcattta cattttctat caatgctatt gaacacatat
     2521 tatagccatt tttatgttca tcaagtttga caactaagtc tgaatcttgt ttttgtttga
     2581 ttgttacaat tccatcagct tcagaccagt tagctacacc ttcgtatata aaagcataaa
     2641 taagtattct ttttatatct tttattttat taccatttat tcttaaattt tctccttgtg
     2701 tattacttcc tgttctgtca tctccatcta attgagcaaa aggtggattg tctaaactac
     2761 caaaagcatt tcctaatgct tgaacagcac cttttacacc atttttcatc tcaaataggc
     2821 aacccaaatc taaatctata ttactgcttc ttaaagagcc aaaaaaaccc tttttttgta
     2881 ctttttgatt ccaatttaaa ttaactaaga tttcacctaa atcgctattt tccttttttg
     2941 taaggtttat tttttgtccc ttttttaatt ctatagccat agtaatcttc ctccacaaac
     3001 atagatttat ttaaatggta gcacttaata agtcaatatt caacaagatt agaataataa
     3061 tattaaaact atactaaata tactaaaaag taggaatcac aatacctttg tatttttctt
     3121 caattaagct tcttgcttta tctgatgtaa gaacatcttg tagaacttta atcttttcac
     3181 tgtctttatc ttcagttcta aatgctaaaa gatttgcata ctcgtcattt ataggagctg
     3241 tataaattcc gtttttcttg gcatcaagac cagcaggtac agcataagta gtatctataa
     3301 atgcagcagt aacatctgga agagaagatg gaatttgagc aatatctaac tctttgaatt
     3361 gtattccctt tggattttct attatgtcct taggactaac taatccatcc cctttaggta
     3421 acgttaaaag tccaatactt tcaaggtatc taaggttctt agagcaagaa gatgggttat
     3481 tgcttattgc aattgtatct ccttttttaa attcatcaac agattttatc ttataagagt
     3541 aaagtattgc tgggcataca tatagttttt taccagccat tattttgtaa cctttggcct
     3601 taacagcttc ttttagataa ggttcatgtt gaaatagatt tgcatcaatt tcaccattat
     3661 ttaaagcttc gtttggaagt atgtaatcat caaagttttt aacttctaaa gtatatcctt
     3721 tttctttaat aagtggtttt aattcttcta ataactctcc tcctggaact aaagtagcac
     3781 caacaacaat ttttttatca tctttagctt tagaacatcc aaccacagct aaagttagta
     3841 ctagacataa taacaaacat aataattttt tgaatttcat ataaattccc tcctgaatat
     3901 aattgattag ttaaaaataa aataccattc tttatcacac tgcaaaataa aaataagcct
     3961 gagtttaaac tcaggcttta aaagctaatt tagactcatc tttcagtata atactgcagg
     4021 atttagcacc aatgtatata aaaatatact ggttgctggg tttcataggg ccagtccctt
     4081 caccactctg gataagaagt atcaattttt aattatgtta taaatattat catgattttt
     4141 tacgtctgta aacataattt tataaatatt ttaaaatctt taatgtattt tatttaacac
     4201 ctaaaattcc agaaactaga atatcagtat cagcacctaa gaaatagtca tttatttcaa
     4261 tagaatccag agtatttctc aaagcatttt cttcatgcct tataccaata agtttatttt
     4321 ctacaaatgc aacatcacat tttccaaaaa aatctccaaa aaattttata ctagttatta
     4381 tgcctttatc aacatttaaa ttaaactcaa catttccacc agaatatttt aattcatttg
     4441 aaagagtata ttttggtgaa ttaccaaaat tccaatccca agtttcgtat tttgatttag
     4501 taagcttttc tatttgtttt atatcatgct cattcaaaca ataattagta ttatcagtat
     4561 ttgttttaga aagataatcc attatagcat ctttaaattc taatatgtcc atttttgaat
     4621 gtaaatgttc acttatattg gttacacgag attttacaga ttttattcct tttccttcaa
     4681 attttatagg tttaaccttt aatgcttttg agaggtcatt tatttcagat gaaaataaaa
     4741 gagtaccatg atgcattact ttgtttttgt agttgtattg agcatttcca gaaaatttct
     4801 tgccatctat aagaaggtca tttcttccag aaaattcggc attgacatct agtgttttaa
     4861 gtaaatctat tatgggctga gtgaatcttc tgaaatcact aaaattatta ttgttacaag
     4921 ctataaatgt aaaatttata tttcccaaat catgaaatac agctccacca ccagactgtc
     4981 tcctaacgac aggtatagaa tattctttta tatattctaa atttatttca gaaagtgtgt
     5041 tttggttttt tccaacaatt atagaaggtt cattcctcca taaaataaat aagtcttcat
     5101 caaaattttt tagaagatat tcttccattg ctaaattaaa ataagggttt gttttttcat
     5161 tataaattaa taacattaaa gtagttcctc catagaattg ataaaatttt tattatgaat
     5221 acaattaatt ttataattcg acaataatat aaaaactcct ttaaattaga aattttaaaa
     5281 actaccttgc aaagtttaaa taaataatat atactcaaaa tatgtttaat aaaagtgaat
     5341 agaagggtag aggcgcaata atctatcagt actaataagg agttaagcac agagagttat
     5401 taggaaagga gatattgccg aagttttata atcgatgctt taagattata ttgctggggg
     5461 tacatagaaa atatgtacaa ctgtcacagg tttatttgtg gtgagctatc atttaaggaa
     5521 ttatattata tttattttta ctattgttga tatattgtaa tgtttaagcc ttgagtgttt
     5581 accactcaag gcttttttag ttttattgta acataatacc ctgtacagtt cacaaaatta
     5641 aaaggagagg gagatttata tgaggaacaa aaaaattaat attatttttc tgacgacaat
     5701 catgttcata atgtcaacag taatggtttt tgcagaagaa gatatagaca ctattgcact
     5761 agctaatgca gaaaaatttg ggattttaac tttaatacca ccattagttg ctataatact
     5821 tgcatttata actaaaaacg taataatttc tttacttata ggtattcttt ctggtagttt
     5881 tataattaaa gctagtggta ttaatgtttt tgcgacattt atacaggcat ttttagattt
     5941 agtagataga gctttagtat ctttagcaga cccttggaat gcagggataa tactacaggt
     6001 tttggcaatt ggaggagtaa taaatcttgt tgctaaaatg ggaggagcta aagctatagc
     6061 tgaagcactt gctaaaagag caaagtcagc aaaagggaca cagcttataa catggttttt
     6121 ggggctttta gtattctttg atgattatgc caattcacta atagtaggtc ctatgatgag
     6181 acctgttgca gataaaatga aaatatcaag agaaaagcta gcatttataa tagatgccac
     6241 agcagcacca gttgctggtt tagctataat atctacatgg ataggtcttg aggttggttt
     6301 aatacatgat gcttttgaaa gtataagtat agatgtagat gcatttggta tatttttaaa
     6361 tacaatacca tttagatttt acaatatatt aatattagct ttcattgtaa tatcagcatt
     6421 attattaaaa gaatttggtc caatgagaaa agctgagata aaatctagaa gtagaaaaat
     6481 tagtatagac cttgatgaag gcgtagaaga gttggatgat ttagctccaa aaaatggagt
     6541 aaaattgagt gtatggaatg ctataatccc aataggaaca ttgataatag ttgcattagc
     6601 atcattttat tatagtggat atacatctat aatgggagga gacgataagg ctctgataca
     6661 actatttaca aattctccat attcttttga agcaataaaa gaagcattta gtgcatctga
     6721 tgcatctaga gcattatttc agtctgcact tgttgcaagt ttagttgcta ttataatggc
     6781 tgtagtaaaa aagattttta caatatcaga agcaattgat gtttggatag atggaatgaa
     6841 aagtttagta atcactggtg taatattaat acttgcatgg tcattaagtt cagtaataaa
     6901 agagttaggt acagctaagt ttttaataca cctattatca ggttcactac caccattctt
     6961 attaccaagt ttaatatttg gtttaggagc aataatatct tttgctactg gaacagctta
     7021 tggaacaatg ggtatactta tgccacttgc aattccatta gcatactcat taaatcctga
     7081 tatgtcttat gtaatagtaa gtactagtgc agttttaaca ggtgcaatat ttggagacca
     7141 ttgttctcca atttcagata caacaatact ttcttcaatg ggagctggct gtaatcatat
     7201 agaccatgtt aacactcaaa tgccatatgc tatatttaca gcagtaatca ctattgtatt
     7261 tgggtatata ccagctggac ttggattgcc aatatatata gtattaccag tggcaatagc
     7321 tgctatattt gtaggtattc aaataattgg taagaaagtt gatgaagcgg aaatagaact
     7381 tgtagaataa agatataaat aagtttcacc ttttattatc actattttgt tagtttttag
     7441 ataattttaa aaagttattg aaaataatgg cgaaatatag taaaataaaa gagtgataag
     7501 cggatgtggc ggaattggca gacgcactag acttaggatc tagcgccatt ggcgtggggg
     7561 ttcgactccc ttcatccgca ctttacagta cttaattgta ctgaaaaaaa ctggggttgc
     7621 tggctttcgg agtgggctat cccgttttta tttttttgta tatataatag attggcactt
     7681 tagtataagg tgcttttttt ataggcaaaa acagtacatt tatttctgtt ttattatcat
     7741 tcatctatta gaatatactt tatttaagaa tgtgttaata agcagtgttc acattcttga
     7801 taaaatgtgg aataatatgc ctaccaataa gcatttggtg tataagtaag agtatttata
     7861 tagttgattg tgaaatgagt acaacaattc tgtacagtat aattggcatt ataattgtag
     7921 cattgaattt aaagtttgca cagtcgctga ttggaaaatt tacaaagtgg atgtcaaaca
     7981 tataaatttt agtttattgg acagttcaag gaatcagaaa ttggaatttt atagtattaa
     8041 tattttatag tattaatata atgtataaaa ttttatagca atgtatttca taagtataaa
     8101 gtgactagaa ttttaagaaa tatacaaatt ctataaaaac taaataattt caagtatatt
     8161 ttaaaaatct tatttttcac aagtttaaaa ttgtaaacga tttcatcaag tctatgaata
     8221 tacttttaac atgttatagt aaatctcaat ataaatttac atttggttct ttgtgtgtta
     8281 ttttaataat aaattaagtg aaaatgttat tttacattaa tatataatat ctttgcataa
     8341 gagtgaataa tataaaagag aaagaattga taataaaaac ttaacaaaaa aattaataca
     8401 taaaaaaaaa taaatataat gtgttgacat ttaattgaaa tattcataca ataaaattga
     8461 aaataaatat ttccaaatga gattagcgag agagtccttt atacgaaaag gcgccgaaga
     8521 agaaatttaa gaaatgccaa tttttagaga agctttcagg caaaaatatc gctaattgat
     8581 gggactctgg agagacttaa gttaagatac tataagtcac cgaagaagca aaataatatt
     8641 aaattattaa atctttcagg tatacaaaca gggatatata ggctaggatt attttccatg
     8701 ctgatatatc ttttttttat gaaaaagtac aggaaataat atcaaagtta cttaaataca
     8761 gtaggtctaa ttttctaggg agggttatat atgaatgaat tgaaaagagt atctttgtac
     8821 aatatacaca aagagcttgg agcaaaactt gttgaatttg ctggctggga aatgccttta
     8881 gaatatgagg gtataaataa agagcatgag aaggttagaa aaagtgcagg tatatttgat
     8941 gtatctcata tgggagaggt acagataaag ggagcagagt cagaaaaatt tatacaaaac
     9001 ctagttacaa atgatataag cacattaaag ataaatgaca ttatatatac acctatgtgt
     9061 tatgaaaatg gtggagtagt tgatgattta ttaatatata aatttggtga agaagattat
     9121 ttattagtaa taaatgcagg taatatagat aaagatgtag catggataat aaaacaaagt
     9181 gaaggataca atgtagatat aaaaaatata tctagtgaag tgagtcaatt agcaattcaa
     9241 ggacctaaag cagaagaaat tcttcaaaaa atcacagata tagacttaaa ctcaataaaa
     9301 ttttataagt caattccatc aacaaaagta tgtggttgtc cttgtttagt ttcaagaaca
     9361 ggatatactg gggaagatgg gtttgagata tactgtaaaa ataaatatgt agaaataatt
     9421 tggaatgaag ttctaaaggt tggaggagaa gatatatgtc cagctggtct aggttgtagg
     9481 gatactttga gatttgaagc agctctacct ctatatggtc atgaaataaa tgaacacata
     9541 tctccaattg aaggtggttt atctatattt gtgaaaacaa ataaagaatc atttattggt
     9601 aaatcaattt tatcaaaaga aaaagaaagt ggagctaaaa gaaagttagt aggatttgaa
     9661 atgcaaggta aaggaatgcc tagaaatgga tatgatataa ggattggtga taaaacagtt
     9721 ggatttgtaa caacaggatg tgcatctcca acgacaggaa aaattttagg tatgggaatt
     9781 atagattctg aatatgcaaa agtaggaaat gaaataggta tagcaatcag aaaaaaagtt
     9841 gttcctgctg tgatagttaa gaaacctttt tataaaaaac aatataaaaa agataatatt
     9901 atattaaaca aagaaaataa attttcatat atacctgcta caagtgaaga taaatcaaaa
     9961 atgttaaaag tagttggatt aaattcggtt gatgaattat tttcagatat accagaagaa
    10021 gtaaaattaa aaagagattt aaatttggaa attggaaaat ctgaattaga ggtaagcaaa
    10081 atagtaaaaa gattgtcaga agaaaactta agtttagaag acttgacttg ttttcttggt
    10141 gctggtgcat atgaccatta tataccatca attataaaac atataacttc aagatctgaa
    10201 ttttatacag catacactcc atatcaagct gaaataagtc agggaacttt acaagtagtt
    10261 tttgaatttc aatctatgat tgcagaaatt acaggtatgg aaattgcaaa tgcatcaatg
    10321 tatgatggag caactgctgc aatagaagca tgtataatgg caatgaatca gactagaaaa
    10381 agtaaaatag tagtgtcaaa aactattcat catgaaactt tgtcagtatt aagaacttac
    10441 ttacaatata aagattgtga gatagtagaa attgactttt gtaatgaata tgggactaca
    10501 gatatagaaa aattaaaagc ttctgtagac aaggatacag catgtgttct tatacaaact
    10561 cctaatttct ttggaattat tgaagaaatg gaagaaatag aaaagataac tcatgaaaat
    10621 aaggctatgt taattatgag tgtagaccca atatcattag gagttttaaa aacaccaggt
    10681 gagataggtg cagatattgt tgttggagaa gctcaatcac ttggaaaccc tcttaatttt
    10741 ggaggtcctt atgtaggatt tttagctagc aaatctaaat atactagaaa aatgccagga
    10801 agaatagttg ggcaaagttt agatgtagaa ggtaaaattg catatgtatt aactttacaa
    10861 actagagaac agcatgtaag aagagaaaaa gcaacttcta atatatgttc aaatcaagct
    10921 ttaaatgctc ttgtagcttc aatatatatg gctactatgg gtaaagaagg atttaaagag
    10981 gttggtatgc agtctatgaa aaaagcacat tatacttaca ataagcttgt acaaactgga
    11041 aaatataagc caatatttaa aggtaagttt ttcaaagaat ttgctgtaca aggaaatctt
    11101 aatatagaaa ctataaatga taagctctta gaagaaaata tattaggtgg ttataattta
    11161 gaatacaatt atccagaatt aaaaaattca actttgcttt gtgtgactga gaaaagaagc
    11221 aaagaggaaa tagataagtt agttggaata atggagggat tataatgaaa gaatacaata
    11281 gtttgttgat agatatatct aaaaaaggta ggaaagcata ctcacttcca aagttagata
    11341 tagatgatat aaaaatagaa gatatgattg accaagatat ggcaagacaa agtgagttga
    11401 atttgccaga agttggtgaa ttagaattag ttagacatta tacattatta tcaaataaaa
    11461 actttggagt tgatacaggt ttttatcctt tgggttcttg tactatgaaa tataatccta
    11521 aaataaatga agatatggca gcacttccaa actttactgg aatgcatcca taccaatctt
    11581 cggatacagc tcaaggttct ctttctttga tgtatgattt atcaagaaga cttgcagaaa
    11641 ttacaggtat ggatgaagtt acactacagc cttcagcagg ttctcatggt gagtttacag
    11701 ggcttatgat tataaaagca tatcatgaaa atagaggtga ccataagaga actaaagtga
    11761 taattccaga ttctgcacat ggaacaaatc cagcaagtgc agctatggca aactttgatg
    11821 tgattcaaat agcatctgat aaaaatggag ctgtagacat aaatgcatta aaagaggttt
    11881 tgaatgatga agtagcagct cttatgctta ctaatccaag tactcttggt ttatttgaaa
    11941 aaaatataaa agaaatagct actcttgtac atgaagcagg aggacttttg tactatgatg
    12001 gtgcaaacat gaatgctatt atggggataa caagacctgg agatatgggg tttgatgtgg
    12061 ttcatcttaa tcttcataag actttttcaa ctcctcatgg aggtggaggt ccaggagctg
    12121 gtcctgttgg agttaaaaaa gaattagtac cttttttacc aataccatta attgaaagaa
    12181 aagaagataa gtatgtacta aattatgata gagaaaaatc tattgggaaa attaaaaatt
    12241 tctatggtaa ttttggagtt cttgtaagag cttacacata tatactaact atgggaaggg
    12301 atggacttaa agaggctagt gaaatggcag ttttaaatgc taattatata aaggaaagta
    12361 taaaagatga ttatattttg ccaatagata ccttgtgtaa gcatgagttt gtattaggtg
    12421 gattgccaag aggagaggcc aatataaaaa ctatcgatat tgcaaaaaga ctactagatt
    12481 atggatacca tcctccaaca atgtactttc ctcttattat aaatgaggca cttatgatag
    12541 aacctacaga aagtgagagt atagagacat tagatagttt tatagaagct atgaaaagtg
    12601 ttgctaaaga ggcaaaggaa gaaccagaac tattgaaaac tgctccacat aatactttgg
    12661 tcaaaagagt agatgatgca agggcagtaa agaaacctat actaacatgg tcacaaagat
    12721 aaataattaa aaagtagaga tttaaagtat aaagagaatt aaatttgtaa gaaaaagcta
    12781 tctaacttaa tccagtttag atagcttttt tgtatattag tatgtatggt attttctata
    12841 taaagtttta aattatatta gtgcttatat agaatattga tatgatgatt atgtatagat
    12901 attaataaga attgttgaat ttatcttgcc ttaactttat ttactctttt atcttaagaa
    12961 aaacttaact aaaatatagc gaaattcata aaaatttagg aaatcttaag aattataata
    13021 tttaaatctt tagatttata ttttattata taaactatag aataataaaa tataaaaatt
    13081 gagaatttta taatatattt gtagatttga gtataaaaaa gtgctaagtt gaaaggagta
    13141 aaatatggat aaggttaatt tagaaaggta tagttgtgat ataaatgttg gtttaaataa
    13201 aaatcaactt caaagtagaa tagatgataa tttaattaat gcatttgata atggaaaaac
    13261 caaaacatat aaacaaattt ttaaagacaa tatatttaca ctttttaatc tcataaatat
    13321 tgttttagtc tgtttattag tttcagttgg ttcttttaaa aataccttat ttatatttat
    13381 tgctgtaatt aatactatta taggagtaat acaagagata agagctaaaa gattattgga
    13441 caatctatct gttatagttt caaataaggt atctgtcata agagaaaaag agaaaaaaga
    13501 gattgatgta caagaattag tattagatga tattatgatt ttaaaaacag gtaatcaaat
    13561 ttgtgcagat tcaaaagttt taaatggaaa attagaggta aatgagtctt tggttacagg
    13621 tgaatctgat attattataa aaaataaaga cgatttttta tattctggaa gttttgtagt
    13681 ttctggagaa gcttttgtaa gagttgaaaa tataggaaaa gataactatg caaataagat
    13741 agttaatgat gcaaagattt ctaaaaaaca taattctcaa cttagaaatt cattaaattc
    13801 aatattaaaa attgtaggta taataataat tcctttagga attatattgt ttgcaaaaca
    13861 gcattttgga aatggagatt ctatagctac atctgtagta ggtacagtgg cagctgtaaa
    13921 tggtatgata ccagagggat taacattact tacgagcatt gctctagctg taggaactat
    13981 aaaacttgct agtcataaaa ctttagtgca agaactgtac tgtgtagaga ctcttgctag
    14041 agttgataca ctatgtcttg ataagacagg tacaattaca gaaggtaaaa tgcaagttga
    14101 tgatatagta atgttggaag aagtggatat taatgaaata atggggaata tatgtaattc
    14161 tctcaaagat gataatgcaa cactaaatgc aataagagat aagtataatg ttttagatac
    14221 gtataaagct aagaatttaa taccattttc ttcagaaaga aaatacagtg gggttacctt
    14281 tgaaaataaa ggtacatatt tattaggtgc atttgagttt atatttaagg aaaaaaataa
    14341 aaaaattaga atagaagttg aaaaatattc taaaaaagga aacagagtaa tagttttagc
    14401 tcatagtgat tcttatatgg aagataataa tattcctgaa gatataaagc cactagcttt
    14461 tatattgata ttagataaaa ttagagatga agctaaggaa actttagaat ttttttacaa
    14521 tgaaggtgta gatataaaaa ttatatctgg tgacaatcct ataacagtaa gtgaggtagc
    14581 tagaaaagca ggtcttaaaa cggctagtaa ttttattgat gcaacaacac ttaaaaatga
    14641 taacgatata tacgaagcag ttgataaata cagtgttttt ggaagggtaa gcccacaaca
    14701 gaaaaaacaa atgattttag cattaaaaaa acaaaatcat acagtagcta tgactggaga
    14761 tggtgtaaat gatgtgctag cgcttaaaga agcagattgc agcattgcca tggcatctgg
    14821 aagtgatgtt actaagaatg tttctaattt agtcttatta gattcaaact ttgcatcaat
    14881 gccaaaggtt gttatggaag gacgaaaggt aattaataac attcaaagag catcttcttt
    14941 gtttttagta aagacaattt tttcattctt cttgtcacta ttaacattat tcttcttcag
    15001 tagacagtat ccatttgttc ctatacaatt atcactaatt ggagctgtaa cagtgggtat
    15061 accatcattc ttcttagctc ttgaagcaaa caaaagtaga atttcaggaa actttttact
    15121 caatatatta aaaaatgctt taccaggagc actatgtgtg acagtaaatg taattattgt
    15181 ttacaatata gttgaatatt taggctatag ttcaagtgta ttttctacaa tgtgtgctat
    15241 attaactggt gtatgtggtc ttttaatatt aagacaacaa tgtttgccat ttaataaaga
    15301 aagattattt ttaattgtaa gtatgactat tgcatttgtt attgggattc tatttttagg
    15361 tggattattc tcatttgtag cattgagtag agaattaatt ctcataacta tggcattagc
    15421 aatattaatg ccaattatga taaaagttat gttatttgtc ttggatgaga tattagagac
    15481 aattgttcca gatttaaatt aattttaaaa tgatataaat aagagcagtt ttagttgaac
    15541 tgctcttttt agatgtgttt aattttatat aagaatatgt atttttatta atcagctata
    15601 tatgagcaac agtagtcaac aacttcatta acctttaaat caaaacttga atcactagga
    15661 acattaaatt tttgcccttc tttataagtt acaaactcat tgcttccagg cagttttaca
    15721 tccatacttc cagcaagcat ctccattatt tcttcttctc tagtagaaaa agtatattct
    15781 cctggcaaca ttaatcctaa agtttttctt tctccattag gcagtattat tactctgcta
    15841 cttaccttac catcaaaata aacgtttgcc ttctttactg cagtaacatt cttaaattca
    15901 ctcattagat aaaacctcca aaattatttg tacatatata ttatataaaa ataatgtaaa
    15961 aaatgctata tatttgcaaa aaaaaattta gtatttatta aaaaaattaa taagaataaa
    16021 aaagagatga aattgcttaa attgataaaa aatataaaat aaattgcaaa gtaagcaaga
    16081 aaagaataag ttgaagagtt atatattata aatattaaaa attttagtat taataaaaaa
    16141 gatattacat taatatgaaa atataggaga ttagaagtgt ttgataatta cagtttatat
    16201 attataataa aataaattgt taattttaaa agaggtgaat tttatggcac atatatgctt
    16261 tgaggatatt tgttatttgt tattaaaaga agcaatgaag ttaaagttaa taccacataa
    16321 taaaattgta ttatttgaag atgatttatc aataggtaat atttttgatg taaataacta
    16381 cgaattaaga gaaaatattt ttaagattag aaatgttttt gaaaataatc cgaaactatt
    16441 attcaagcca gatttgttag aagataggaa attatcaaaa tgtagaaata taataatatg
    16501 gtgtgcaaat aacccaaaag atttgtgtgg agtatattat gtagtgagta agtttcaaga
    16561 taaaaatata aagattgcat tagtagatga aaaaaagaca gaagatgctc taattagata
    16621 ttcatttacc agtgaaatgt ctccagaaga ttttccattc tttttagata agacactaga
    16681 aattggcttt aatgaaaaac tagaatttaa aaataaatgg gatgtgttag ttaaagaaaa
    16741 ttctattttt agagcattag taggtaacga aattaaaagt gttgaagaaa cttattttga
    16801 taaatatata ttaagaaata tcagtgacaa atggcaacct ttaattgaag tggttgcaaa
    16861 ctgtatgttt gaagatgaaa tgcgtgtaaa agattggttt atactttgga gattagagaa
    16921 gatggcaagt ttaaaaatag taaaaataaa aggaagtaaa ggggattttt attctgataa
    16981 ggagataaga aaagtttaaa aattgaattt ataataaatt gtattaaata atgggtataa
    17041 ctatgaagaa aaatatctta acagtattaa tattataatt agcaa
//
LOCUS       DAELXX010000088        16733 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.088, whole genome shotgun sequence.
ACCESSION   DAELXX010000088 DAELXX010000000
VERSION     DAELXX010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16733
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.088"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..759
                     /gene="tuf"
                     /locus_tag="KPH29_002721"
     CDS             <1..759
                     /gene="tuf"
                     /locus_tag="KPH29_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBG1468304.1"
                     /translation="ELLELVEMEVRDLLTEYDFPGDDTPIVRGSALMALEDPKSEWGD
                     KIVELFEQIDEYIPAPERDTDKPFLMPVEDVFSITGRGTVATGRVERGVLKVQDEVEL
                     VGLTEAPRKVVVTGVEMFRKLLDQAQAGDNIGALLRGVQRNEIERGQVLAKTGSVKAH
                     TKFTAEVYVLKKEEGGRHTPFFDGYRPQFYFRTTDVTGACKLPEGIEMVMPGDNVTME
                     VDLINSIVVEEGLRFSIREGGRTVASGVVATIIE"
     gene            1009..1158
                     /gene="rpmG"
                     /locus_tag="KPH29_002722"
     CDS             1009..1158
                     /gene="rpmG"
                     /locus_tag="KPH29_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBG1468305.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
     gene            1184..1405
                     /gene="secE"
                     /locus_tag="KPH29_002723"
     CDS             1184..1405
                     /gene="secE"
                     /locus_tag="KPH29_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBG1468306.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            1425..1967
                     /gene="nusG"
                     /locus_tag="KPH29_002724"
     CDS             1425..1967
                     /gene="nusG"
                     /locus_tag="KPH29_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HBG1468307.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDVDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            2003..2428
                     /gene="rplK"
                     /locus_tag="KPH29_002725"
     CDS             2003..2428
                     /gene="rplK"
                     /locus_tag="KPH29_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBG1468308.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            2498..3196
                     /gene="rplA"
                     /locus_tag="KPH29_002726"
     CDS             2498..3196
                     /gene="rplA"
                     /locus_tag="KPH29_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBG1468309.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     misc_feature    3248..3376
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            3419..3925
                     /locus_tag="KPH29_002727"
     CDS             3419..3925
                     /locus_tag="KPH29_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBG1468310.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     gene            3983..4348
                     /gene="rplL"
                     /locus_tag="KPH29_002728"
     CDS             3983..4348
                     /gene="rplL"
                     /locus_tag="KPH29_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBG1468311.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
     gene            4740..5528
                     /locus_tag="KPH29_002729"
     CDS             4740..5528
                     /locus_tag="KPH29_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBG1468312.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            5999..9715
                     /locus_tag="KPH29_002730"
     CDS             5999..9715
                     /locus_tag="KPH29_002730"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBG1468313.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIIKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGQSFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     VEATEDEDIENLEEFTEDDLFEEEIDFESDDFDM"
     gene            9757..13242
                     /gene="rpoC"
                     /locus_tag="KPH29_002731"
     CDS             9757..13242
                     /gene="rpoC"
                     /locus_tag="KPH29_002731"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBG1468314.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVLVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            13531..13953
                     /gene="rpsL"
                     /locus_tag="KPH29_002732"
     CDS             13531..13953
                     /gene="rpsL"
                     /locus_tag="KPH29_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBG1468315.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            14076..14546
                     /gene="rpsG"
                     /locus_tag="KPH29_002733"
     CDS             14076..14546
                     /gene="rpsG"
                     /locus_tag="KPH29_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBG1468316.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            14595..16661
                     /gene="fusA"
                     /locus_tag="KPH29_002734"
     CDS             14595..16661
                     /gene="fusA"
                     /locus_tag="KPH29_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBG1468317.1"
                     /translation="MARKFPLEKTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFINKMDILGADFYNVISMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         6564 a         2082 c         3439 g         4648 t
ORIGIN      
        1 gagttattag agttagtaga gatggaagta agagatttat taacagaata tgatttccca
       61 ggagatgaca ctccaatagt aagaggttca gcattaatgg cattagaaga tccaaagagc
      121 gagtggggag ataagatagt agaattattc gagcaaatag atgagtatat accagctcca
      181 gagagagata cagataagcc attcttaatg ccagtagagg atgtattctc aatcacagga
      241 agaggaacag ttgcaacagg aagagtggaa agaggagtac taaaagtaca agacgaagta
      301 gaattagtag gattaacaga agcaccaaga aaagtagtag taacaggagt agagatgttc
      361 agaaaattat tagaccaagc acaagcaggg gataatatag gagcattatt aagaggagta
      421 caaagaaacg agatagaaag aggacaagta ctagcaaaga ctggatcagt aaaggcacac
      481 acaaagttta cagcagaagt atatgtactt aaaaaagaag agggtggaag acatacacca
      541 ttctttgatg gatatagacc acaattctat ttcagaacaa cagacgtaac aggagcttgt
      601 aagttaccag aaggaataga gatggtaatg cctggagata acgtaacaat ggaagtagac
      661 ttaataaact caatagttgt agaagaggga ttaagattct caataagaga aggtggaaga
      721 acagtagctt caggagttgt tgctacaata atagagtaat tctttactaa actgatggat
      781 taaaaggaat aacaaaaaga ggggttaggc tcctcttttt ttaacataga aatacaaaaa
      841 cattgacaca gcataggatt ttgtgataac ttatacaagg taatgattat aggaagttaa
      901 taatatcttg aatactgtag atttttaata aatacatatt ttaaagatat tatattcaca
      961 tatattaaca atatatatta ttgtattaca aagttggagg tgtatcagat gagagttaaa
     1021 gtaactttag catgtacaga gtgtaagcaa agaaactaca acactactaa gaataagaaa
     1081 aataatccag acagaataga attacaaaaa tattgtagat tctgtaaaaa gcatacaact
     1141 cataaagaaa caaaatagtt ttttataagg atgtgagtat ggaatggctg cccaaaaaaa
     1201 tgacggtgca aaaactaaaa aaaggtttag tttatttgga tatttaaaag aaactaagca
     1261 agaattaaaa agagttacat ggccgacaaa aaaggaactg tttaaaaaca caagtatagt
     1321 tttaactgta gttatttcat gcactatatt agtatggggt atagacacta tattatctgg
     1381 tgcactagcg ttattactaa aatagacgaa aagaggtaag ttaaatgtca gaattacaag
     1441 aagcaagttg gtatgtagtt catacctatt cgggacatga aaataaagtt aaagctacaa
     1501 ttgagaaagc agttaaaaca agaggtatgg aagactgcat aagacaagta gttgttccaa
     1561 cagaagaggt agtggaaact actaaaactg gaaaagaaaa aactagacaa cgtaaagttt
     1621 atccaagtta tgtcttggtt aaaatgataa taactgatga atcttggtat gtagtaagaa
     1681 atactaaggg agttacaggt ttcgttggac ctggttcaaa accagtacca ttaagtgaag
     1741 atgaagtcaa agctatgggg atagatacaa cagaccctaa agtagtaaat agcgatgttg
     1801 attttgaaat aggagacaca gtaaaagtat ctcaaggacc atttagtgga caaataggta
     1861 acatagaaga aattgactta gaaaatagag aagtcaaagt atgcataaat gcatttggta
     1921 aaaggactct atttgtaata gagcttgaag gtatagaaaa aatctaatac tatatagaaa
     1981 aatctgagga ggtgcgctca aaatggctaa aaaagttata ggtcaaataa aattacaaat
     2041 acctgcagga aaggctactc cagcgccacc agttggacca gcattaggac aacatggtgt
     2101 taatataatg ggattcacaa aagaatttaa tgctaaaact gcagatcaag ctggaatgat
     2161 aataccagtt gttataactg tatatcaaga tagatcattc agttttataa caaaaactcc
     2221 accagctgca gttttaatta aaaaagcatt aaacttaaaa tcaggttctg gagaaccaaa
     2281 caaaaagaaa gttgctaaaa tgacttcagc tcaagtaaga gaaatagctg agttaaaaat
     2341 gcctgactta aatgctgcat cagtagaagc tgctatgagt atgatagctg gtactgctag
     2401 aagcatgggt gttgtaatcg aagactaatt aaaagataca ctttttttat aaagtgggag
     2461 gcgagaagcc gaaattaacc acaaggagga aaataaaatg gctaaaaaag gtaaaagata
     2521 tgcaggtgca ttacaaaaag tagatagaac taaattttat gatgcatcag aagcattaac
     2581 attagtttca gatatagctg gagctaaatt tgatgagaca gtagaagcac acattaaatt
     2641 aggtgttgac tcaagacatg ctgaccaaca agtaagaggt gcagttgtat tacctcatgg
     2701 aactggtaaa acaaaaagag ttttagtttt tgctaaaggt gaaaaagcta aagaagctga
     2761 acaagctggt gctgactttg taggagctga agaattagtt caaaaaatac aaggtgaaaa
     2821 ctggtttgac tttgatatag tagttgctac tccagatatg atgggtgtag taggtagatt
     2881 aggtagagta ttaggaccta aaggtttaat gccaaaccct aaatcaggaa cagttacatt
     2941 tgatgtagct aaagctatag atgaaataaa agctggtaaa gttgaataca gattagacaa
     3001 aactaacata atacacgttc cagtaggaaa agtatcattt ggtggagaaa aattaactga
     3061 aaactttact gcattaatgg atgctataat aaaagctaaa ccagctgctg ctaaaggaca
     3121 atatttaaga agtataacag tagcttcttc aatgggacct ggagttaaaa taaacccagc
     3181 aagaacagct gaataataaa actgttgaca caggttgaca aaaatgttat tatatcactt
     3241 gtcaatgtaa aaaagaatag atttgccgta gatagtgggt gcgaaagctt aatatcccgc
     3301 cgaggtttta gataaattga gattactaaa gcttttgatg tctacttttg gcaacaaaag
     3361 ctttttataa tgttgcggta aatattaagc ctatttggtg aaggaggtgc acatctatat
     3421 gagaaaagct atagaaatta aatcagaagt tgtttctgag atagttgaaa agttacaaaa
     3481 atcttctgct gctgttgttg tagattacaa aggattaaca gttgaagaag taactgagtt
     3541 aagaaaacaa atgagagaag ctggtgtaga ttacaaagta tacaaaaata ctttagttag
     3601 aagagcagct aaagaagttg gtatagagca atttaatgat gaattattag ttggaactaa
     3661 tgccatagca tttggatatg atgatccagt tgctccagct agaatattaa aaggattcat
     3721 ggattctcat ccaaaaatga aattaaagat gggtattgtg gaaggtgcat tctatgatga
     3781 atctaagatt gttgaaatgg ctaacatacc atcaagagaa gttcttatcg ctaaattact
     3841 tggtagctta aaagctccag tatcaaactt cgcatactta atagatgcaa tagcgaaaaa
     3901 ggcagaaggt caagaagaag cttaattgct taactaaaaa attataaata ttaatttgaa
     3961 ataaaattcg gaggtgctaa aaatgacaat agaacaaata ttagaagcta tagaaaatat
     4021 gaaagtttta gaattaaatg aattagttaa agctgctgaa gaaaaattcg gtgtatcagc
     4081 ttcagctcca gtaatggtag ctggtgcagc tgctggtgga ccagctgctg aagaaaaaac
     4141 tgagtttgat gtagtattaa ctgacgttgg ttcttcaaaa gttggagtta taaaagcagt
     4201 tagagaaata acaggattag gattaaaaga agctaaagaa gtagttgaca atgctcctaa
     4261 gacagttaaa gaaggagctt ctaaagaaga agctgatcaa ataaaagaga aattagaagc
     4321 tgctggagct aaagtagaag ttaagtagtt tctttaaaaa gatacccatt gggtatcttt
     4381 ttttatttat aaaaaactat aaagaaagta tttggaatta taaagaaagt gtttttataa
     4441 aataaaaaaa atttatataa tagtactaaa ttaagggtat catatatggt gtaatggtat
     4501 tttaatttaa cattattatc aaattaaaat ataaacaatt gttgtaatgt ctgtatataa
     4561 agtatataat taaactaaaa tgtaaaattt tacattttag tttaaaaaaa tacacgagtg
     4621 ttttataaat aaaatgtaat aaataattaa ttaacaaata taaattttaa aataagttag
     4681 atatttagaa aaatcgaaat ttgtaggatt taaaggacat attcaaggag gagttaataa
     4741 tggaaaaatt acaaggaaaa attgcagtag ttactgcagc aacaaaaggt attggattag
     4801 catcagcaga gatattagca aaaaatggag caactgtgta cttagcagct cgttcagaag
     4861 aattagctaa tgaagttata aataaaataa gtgcagaagg tggttgtgct aagtttgttt
     4921 actttaatgc tcgtgaagaa gagaccttta cctcaatgat agaagaggta gttaaaaaag
     4981 aaggtaagat agatatactt gtaaacaatt ttggttcaac aaacccttct cttgacaaag
     5041 accttgtaac tggggataca gataatttct ttgacacagt aaatactaat ttaaaaagtg
     5101 tgtatttacc atgtaaagca gcaattcctc atatgataaa gaatggaaaa ggaagtatag
     5161 taaatatatc aagtataggt tcagtattac ctgatttatc tagaatagct tactgtgtat
     5221 caaaagcagc aatcaactca ttaactcaaa acatagctac acaatatgct aaagacaatg
     5281 ttagatgtaa tgctgtactt ccagggctta ttgcaactaa agcagcatta gacaatatgt
     5341 ctccagagtt cataaaagaa tttttaaagc atgttccttt aaatcgtata ggggaaccag
     5401 atgatatagc aaaagctgtt ctattctatg ctagtgatga ctcatcattt ataacagggg
     5461 atttacttga agttgcagga ggatttggtt tacctactcc acaatttgca gataatatat
     5521 taggataatt aaattactaa tatgcaatga aagcttatgc attagttata gtagaaatga
     5581 tatttttata aaggccatag atataattta tattggaaag gtttgaggtt caaaattcca
     5641 agttaaattt atttatggtc tttattttgt atattttaat aaattgtata tatttgtata
     5701 aatgtaatta tttacaatca ttttaatatg gaattataat taaatagggg ggatggtact
     5761 tgtataaaat tttttataga aaattagacc tataaatgaa aaatatggat tttatattgg
     5821 aaaattgtac ataatacata atatatgctt aaaataattt acaaaaaatc aaaaataatg
     5881 catagtttac atataagata aaaaatatat tgaaaaatac ttgacattat gctaaaatta
     5941 ttagatgcgt tagaatgaac tattgtttta tttttaatta ttgagaggtg aaaagtgaat
     6001 gccacatcct gtcacgatag gtaaaagaac tagaatgagc ttttctaaaa ttaaggaaat
     6061 agctgatgtg ccaaatctta tagaaattca agtagattcc tatgagtggt ttttaaaaga
     6121 aggtttaaaa gaagtgtttg atgatatttc accaatagaa gattatactg gtaatcttat
     6181 attggaattt gtcgactatt ctctagatga taaaccaaag tacgatatag aagagtgtaa
     6241 agagagagat gctacatatt gtgcacctct aaaggttaag gtaagactta taaataaaga
     6301 aacaggtgag ataaaagaac aagaagtgtt tatgggtgac ttccctttaa tgacagaaag
     6361 aggaactttc gttataaatg gagcagaaag agttatagta agtcaattag ttagatctcc
     6421 tggtgtttac tatgcggaag aaagagataa aacaggtaag agattgattt catctactgt
     6481 aattcctaat agaggagcat ggttagaata tgaaacagat tctaatgatg taatatctgt
     6541 aagagttgat agaacaagaa aacaaccagt tacagtttta cttagagctt taggaatagg
     6601 aacagatgca gagataattg accttttagg agaagatgag agattatctg ctacattaga
     6661 aaaagataat actaagacag tagaagaagg tcttgtagag atatacaaaa aattaagacc
     6721 aggtgaacct cctactgtag aaagtgcatc atctttatta aatgctttat tctttgaccc
     6781 aaaaagatat gacctagcaa aagttggtag atataaattc aataagaaac ttgctttatg
     6841 ttatagaata atgaataaaa tttcagcaga agacataata aatccagaaa ctggagaagt
     6901 atttgtaaaa gctggagaaa aaataagcta tgatttggct aaagccatac aaaatgcagg
     6961 tataaatgta gtaaatctac taatggatga tgataaaaaa gttagagtta taggaaataa
     7021 ttttgtggat attaagtctc acattgattt tgacatagat gatttaaaca taaaagaaaa
     7081 agtacattat ccaactttaa aagaaatttt agatggatat agtgatgaag aagaaataaa
     7141 agaggctata aaatcaagaa ttaaagaact tataccaaaa catatattat tagatgatat
     7201 aatagcttca ataagttatg agttcaatat attctataat ataggaaata ttgatgatat
     7261 agatcaccta ggaaacagaa gaataagatc tgtaggtgaa ttgttacaaa atcaagttag
     7321 aattggtctt tcaagaatgg aaagagttat aaaagaaaga atgacggttc aagatatgga
     7381 agctataacg cctcaagcgt tagttaacat aagaccagtt tcagctgcaa taaaagaatt
     7441 ttttggtagt tctcagttat ctcagttcat ggatcaaaca aatcctctat ctgagttaac
     7501 gcataagaga agattatcag cccttgggcc tggaggtctt tcaagagaaa gagctggatt
     7561 cgaagtgcgt gacgttcacc attcacacta tggtagaatg tgtccaatag agactccaga
     7621 gggaccaaat atcggtctta taaactctct aggaacttat gcaaaaataa atgaatttgg
     7681 atttatagaa tcaccatata gaaaatttga taaagaaaca tcaacagtta ctgatgaaat
     7741 acattattta actgctgacg aagaagattt attcgtaaga gctcaggcaa atgagccatt
     7801 aacagaagat ggtaaattcg taaaccatag agttgtttgt agaactgtaa atggtgctgt
     7861 tgaaatggtt cctgaaagta gagtagatta catggatata tctcctaagc aggttgtatc
     7921 tgttgccact gctatgatac ctttcttaga aaatgatgat gccaaccgtg cccttatggg
     7981 agcgaacatg cagcgtcagg cagtgcctct agttagaaga gaagcaccaa ttataggaac
     8041 tggtatagaa tacagagcag caaaagactc tggagcagtt gttgttgcta gaaattctgg
     8101 tatagcagaa agagtaacag cagatgaaat aattataaaa agagaagatg gaaacagaga
     8161 tagatataat ctacttaagt ttaaacgttc aaactcaggt acttgtataa atcaaacacc
     8221 tataataaat aagggtgacc agataataaa aggtgatgtt atagcagatg gtccagcaac
     8281 tgatttagga gaagttgcac ttggaagaaa ctgtcttata gcattcatga cttgggaagg
     8341 ttataactac gaggatgcta tattaataaa tgaaagatta gttaaagaag atagattatc
     8401 aacaattcat atagaagaat atgaatgtga agctagagat acaaaactag ggccagagga
     8461 aataactaga gatataccta atgttggaga tagtgcaatc aaaaacttag atgatagagg
     8521 tataataaga ataggtgcag aagtagactc aggtgatata ttagttggta aggtaactcc
     8581 aaaaggagaa actgaactta ctgctgagga aagattactt cgtgcgatat tcggagaaaa
     8641 agccagggaa gttagagata cttcacttaa agtacctcat ggtgaatctg gtataatagt
     8701 tgatgtaaaa gtatttacta gagaaaatgg agatgattta tctccaggag taaatgaatt
     8761 agtaagatgt tatatagcta aaaagagaaa gataaaagtt ggagataaga tggctggtcg
     8821 tcatggtaat aaaggggtta tctcgagagt attacctgaa gaagatatgc cattcatgga
     8881 aaatggaacg ccattagata taatacttaa cccacaaggt ataccatcac gtatgaacat
     8941 cggtcaggtt ctagaagttc atttaggact tgctgctaag acattaggat ggtatgtagc
     9001 aacatcagta ttcgatggtg ctaatgagta tgacataatg gatgctcttg aagaagctgg
     9061 atatcctaga gatggtaaat taactttata tgatggtaga acaggtcaat cttttgacaa
     9121 tagaataact gttggatata tgtattactt gaaactacat cacttagtag atgaaaaact
     9181 acatgcaaga agtactggac catactcatt agttactcaa cagccactag gtggtaaagc
     9241 acaatttggt ggacaaagat ttggagagat ggaggtttgg gctctagaag catatggagc
     9301 ggctcacata cttcaagaga tacttactgt taagtctgat gatgttgtag gtcgtgttag
     9361 aacttatgaa gctatagtta aaggtgaaaa catacctgag ccaggaatcc cagaatcatt
     9421 taaggttctt ataaaggaac ttcaaagttt atgcttagat gtaaaagtat tgacagatga
     9481 agaccaagaa atagaagtaa gagaatctgt agatgaagat gatacgatag gtgaatttga
     9541 gctagacgtt gtaaatcaca tgggagaagt tgaagaaagt aatataatag aagaaattga
     9601 agatgatttt gtggaagcca cagaagatga ggatatagag aatttagaag aattcactga
     9661 agatgattta tttgaagaag aaatagattt tgaaagtgat gactttgata tgtaaattta
     9721 attaatgtaa atctcaaata gaagggagag aactccttgt ttgaattaaa caatttcgag
     9781 tcgataaaaa tagcattggc ttctccagaa aaaataagac aatggtctag aggagaagtt
     9841 aaaaagccag aaactataaa ttaccgtact ttaaagccag aaaaagatgg tcttttctgt
     9901 gaaaggatat ttggaccaca aaaagactgg gagtgtcatt gtggtaaata tagaagagtt
     9961 agatataaag gtgtagtttg tgatagatgt ggagtagaag taactaaatc aaaagtaaga
    10021 agagagagaa tgggacatat agagctagct gctcctatgt ctcacatctg gtacttcaaa
    10081 ggtataccaa gtagaatggg acttttactt gatatgtcac caagatcgtt agaaaaaata
    10141 ctatatttcg cctcatatgt agtagttgat ccaggtgaaa ctggattgaa tgaaaaacag
    10201 ttacttacag aaaaagaata cagaactgct cttgaaaagt atggatatac ttttactgtg
    10261 ggtatgggtg ctgaagctgt aaaaacatta ctacaaaata tagatttaga acaacaaagt
    10321 aaagacttaa gagcggaatt aaaagatagt acaggacaaa agaaagtaag aacaattaga
    10381 agattagaag ttgtagaagc atttaaaaaa tctggaaata aaccagaatg gatgatttta
    10441 gatgcaatac cagtaatacc gccagattta agaccaatgg tacaacttga tggtggaaga
    10501 tttgcaactt cagacctaaa tgatttatac agaagagtta taaatagaaa taatagactt
    10561 aaaagactat tagagcttgg agctccagat ataattgtaa gaaatgaaaa aagaatgctt
    10621 caagaagctg ttgatgcatt gatagataat ggtagaagag gtagacctgt aacaggacct
    10681 ggaaatagac cacttaaatc tttatcagat atgttaaaag gtaagcaagg tcgtttccgt
    10741 caaaacttac ttggtaagcg tgttgactac tcaggacgtt ctgttatagt tgttggacca
    10801 gaacttaaat tttatcaatg tggacttcca aagaagatgg cattagaatt attcaagcca
    10861 tttgttatgg ataaattagt taaagaaggt tatgcacata atataaaaag tgcgaaatct
    10921 atagtagaaa aagttaagcc agaagtttgg gatgttttag aagatgttat aaaaagtcat
    10981 ccagttcttc ttaaccgtgc accgactcta catagattag gtatacaagc atttgaacca
    11041 atcttagttg aaggtaaggc tataaaacta catcctcttg tatgtacagc ttataatgca
    11101 gactttgatg gtgaccaaat ggcagtccat gtacctttat cagtagaagc acaagcagaa
    11161 gcaagattct taatgctttc tgtaaataat atacttgctc ctaaagatgg ttcacctata
    11221 actactccat ctcaggatat ggttttaggt tgctattatc taacaataga agcccaagat
    11281 ggagctaaag gaacaggtat ggtatttaaa gactttaatg aactattact tgcttattac
    11341 aataaatcag ttcatctaca tgcattagtg aaactgaagg taacacttga agatggaaga
    11401 agttcattgg ttgaaagtac tgttggtaga tttatattta atgaaaatat acctcaagac
    11461 ttaggttttg tagatagaaa agaaaatcca tttgcacttg aagttgattt cttagcagat
    11521 aaaaaatctc ttggtaaaat aatagataaa tgctttagaa aacacggaaa tacagaaaca
    11581 gctgaattac tagattatat aaaagctcta ggatttaaat attctacatt aggtggtata
    11641 acagttgctg ttgatgatat gagtgtccca gaagaaaaga aagtatttat agctgaggca
    11701 gaagctaaag ttgataagta tgaaaaagca tacagaagag gtctaatctc tgatgaagaa
    11761 agatatgaaa aagttataga gacatggaca gaaacaactg ataaagttac tgatgctctt
    11821 atgggtggac tagatagatt aaataatata tatataatgg cacattcagg agccagaggt
    11881 tctaaaaacc aaattagaca gctggcaggt atgcgtggtc ttatggcaaa tgcatctggt
    11941 aaaacagtcg agataccagt taaatctaat ttccgtgaag gtttatcagt actagaatac
    12001 tttacatctt cacatggtgc cagaaaaggt cttgccgata cagctatacg tacagctgaa
    12061 tctggatatt taacaagaag acttgttgat gtaagtcaag atgttattgt aagggaaata
    12121 gattgtggaa cagaagatac tacagagatt tatgctataa aagaaggaaa tgaagttata
    12181 gaagagatat atgatagaat tgtaggaaga tatactatag accctatatt aaatcctgaa
    12241 actggagaag ttctagttga ggctgattca atgatacaag aagatgaagc agaaactata
    12301 gtagctttag ggattgaaaa aattaggata agaacagttc ttaactgtaa aactaatcat
    12361 ggagtttgtt ctaagtgtta tggtagaaac ttggcaacag gaaaagaagt taatataggt
    12421 gaagcagttg gtataatagc agctcaatct atcggtgagc cgggtactca gcttacaatg
    12481 cgtacattcc atactggagg agttgcagga gctgatataa ctcaaggtct accaagggtt
    12541 gaagaattat ttgaagctag aaaaccaaaa ggattagctg taataactga agtatctggt
    12601 agagttgaaa ttgatgaaac aggaaagaga aaagaagtaa atgtaatacc agaagaaggc
    12661 gagactcaaa catatgtaat tccatatggt tctagattaa aagttaagca aggtcaaatg
    12721 ctagaggctg gagaccctct aacacaagga tttataaatc ctcatgatat agtaagagta
    12781 aatggtgtta agggagttca agaatatata gttaaagaag ttcaaagagt gtatagactt
    12841 caaggggttg atgttaatga taagcatata gaagttatag taagacaaat gctatctaaa
    12901 gtaaaagttg aagacccagg agatacagat ttattgccag gtggatatga agatgtatta
    12961 acattcaatg aatgtaataa agatgctata gataaaggtc taagaccagc agttgctaaa
    13021 agagttttac ttggtataac taaagcatct cttgcaactg attcattctt atcagcagct
    13081 tctttccaag aaacaacaag agtattaaca gaggcagcaa taaaaggtaa agaagaccac
    13141 ttaataggac ttaaagaaaa tgttatatta ggtaagttaa taccagcagg aacaggaatg
    13201 aagaaatata gaaatatagc tgttgaaaaa attgaagatt aatagcatat agagtaaaga
    13261 aatcttgaca accattattt gtggtgttaa aatagtaaag tgtgttgttt aaaagtacac
    13321 taagggcttg aacaagttct tagtgacttt tagatggcaa taaatgagaa ctaaaaagca
    13381 tcaagctaga aaagtgctaa tatcacatat ttatgtgtta tagacattaa attgtaagta
    13441 acgaatgtta caatcagtca gtatgaatat tcatattgat gtagttggcg gtcactgata
    13501 agatcgaaaa tttggaagga ggtgcagatg atgccaacaa ttaaccaatt agttcgtaaa
    13561 agtagaaaag cgctagagaa gaaatctact gctccagcat tacaaaaagg ttacaactct
    13621 ttaaataaga aagtaactga cgctagtgcc ccacaaaaaa gaggggtttg tacttcagta
    13681 aaaacagtta ctcctaagaa gcctaactca gctttaagaa aagttgccag agttagatta
    13741 actaatggta tagaagtttc tgcttatata ccaggagaag gacacaactt acaagagcat
    13801 agtgttgttc ttataagagg aggaagagta aaagaccttc caggggttag ataccatata
    13861 ctaagaggta ctctagatac agcaggtgtt gataagagaa gacaatcaag atctaaatac
    13921 ggtgcgaaga gacctaaaga agctaaaaaa taaaacaatt aatgattgat tatagttcgg
    13981 tgctttaaat atatatatat ttacggcatc acggcattaa atgatttatg tcgagtacct
    14041 atgatttaag ttaaataatt aaggagggaa gcgaaatgcc aagaaaaggt aatattccaa
    14101 aaagagaagt tttaccagat cctatgtatg ggagtaaggt agttactaaa ttaataaata
    14161 atctaatggt agacggaaaa aaaggaaagt ctcaaagaat agtttatgat gctttcgcaa
    14221 ttgttgctga aaagacagga gaagaagctt tagaagtatt taataaagca atggataata
    14281 taatgcctgt tttagaagta aaagcaagaa gagttggtgg ggcaaattac caagtgccaa
    14341 tcgaagttag acctgaaaga agacaaacat taggtttaag atggctagta aaatatacta
    14401 gagctcgtgg agaaaaagga atggttgaaa agctagctaa agagataatg gacgcagcta
    14461 acaacactgg agcttcagtt aagaaaaaag aagatactca taaaatggca gaagctaata
    14521 aagcatttgc tcattacaga tggtaggata tatagatatc ttataaattc aaggatatca
    14581 taaggaggat aaccatggct agaaagtttc ctttggaaaa aactaggaat ataggaatca
    14641 tggctcatat agatgcagga aagactacta ctacagaaag aatcctgttt tatactgggc
    14701 aaactcataa aataggagaa actcatgaag gagcttctca aatggactgg atggagcaag
    14761 aaaaggaaag aggtataaca ataacttctg ccgctactac tgcttcatgg aaagaccata
    14821 gaataaacat aatagatact cctggacacg tggacttcac agttgaggtt gaaagatctc
    14881 taagagttct tgatggttca gttgctgtat tctgtgcaaa aggtggggtt gagcctcaat
    14941 ctgaaaatgt atggagacaa gctgaaactt atggagtacc tagaatagct tttataaaca
    15001 aaatggatat attaggtgca gatttctaca atgttataag catgatgaaa tctagattaa
    15061 attctaatgc agttcctatg caattaccaa taggtaaaga agattcattt ataggtataa
    15121 tagacttatt aaaaatggat gctgttatat acaaagacga cctaggggtt gaaatggaag
    15181 aaacagatat accagaagat atgaaagaat tagctgctga gtggagagaa aaattagttg
    15241 aatcagtagc tgaaactgat gaagaattaa tgatgaaata ccttgaaggt gaagaactta
    15301 ctatagatga attaaaagtt gctataagaa aagctactat agcttgtgaa atgaacccag
    15361 ttttctgtgg tacagcttat agaaacaaag gtgttcagtt agtaatagat gcagttttag
    15421 attacttacc agcaccaact gatataccag ctataaaagg tatattagca gatggagaag
    15481 aagctgaaag acattcttct gatgaagagc cattctctgc attagcattc aaaataatga
    15541 ctgacccatt cgttggaaaa ctagcgttct tcagagttta ttctggaaca ttagaatcag
    15601 gttcttatgt tttaaatgca actaagaata agagagaaag aattggtcgt atattacaaa
    15661 tgcatgctaa tacaagagaa gaaataacta aagtgtatgc tggagatata gcagcagcag
    15721 taggattaaa agatactact actggagaca cattatgtga ccctgctaat ccaataatac
    15781 ttgaatctat ggaattccct gagccagtta tatctgttgc tatagaacca agttctaaag
    15841 cagctcaaga aaaaatgggt atagctcttc aaaaattagc tgaagaagac ccaactttca
    15901 cagttaagac tgaccaagaa acaggacaaa ctataatatc tggtatgggt gaattacact
    15961 tagagataat agtagataga ttattaagag aatttaaagt agaagctaaa gttggtgcac
    16021 cacaagttgc atatagagag actataactc aaccagttga tgttgaatac aagtactcta
    16081 agcaatcagg tggtagagga caatacggtc acgttaagat tagagttgct cctcaagagc
    16141 caggtgaagg atacaagttc acaaacaaaa ctgttggtgg atcagtacca aaagaatatg
    16201 ttggtccagt tgacatgggt atacaaggag ctatgcaatc tggtatagtt gctggatatc
    16261 cagttgtaga cgttgctgtt gagttatatg atggttctta ccatgaggtt gactcttctg
    16321 agatggcgtt taagatggct ggttcaatgg cattcaaaga tgctatgaag aaaggtaatg
    16381 cagttctttt agagccttac ttcaaagtag aagttgttac tccagaagat tacatgggag
    16441 atgttatggg tgacttaaac tcaagaagag gattaatcca aggtatggaa gctagaagtg
    16501 gtgcacaagt tataaatgca ttcgttccac tttcagaaat gtttggatac tcaacagacc
    16561 taagatcttc aactcaaggt cgtgcaactt acacaatgat attcgatcac tatgagcaag
    16621 ttccagcttc agttgctaag aaaatagctg aaggaaaata atttaaaaat aattaataat
    16681 ataaatataa aaaataatca gctagatgag atatatctca tctagcacaa gat
//
LOCUS       DAELXX010000089        16688 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.089, whole genome shotgun sequence.
ACCESSION   DAELXX010000089 DAELXX010000000
VERSION     DAELXX010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16688
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.089"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            249..2561
                     /locus_tag="KPH29_002735"
     CDS             249..2561
                     /locus_tag="KPH29_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG1468318.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKENNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDTTSNKVNYISDELD
                     TTSNKVNYVSDELDTTSNKVNYVSDELGMALHKVNCVSDELDMTSNKVNYVLDELDIT
                     SNKTDYISNELYNLSNKSIKDNIPIKMAVITKSNMLNQLPDLVELERVFSKIKFKVCF
                     DMFMTDTATLCDMFIPCTNTLESEDIIYSSMTNPYITYNERAVKPAHKLMDEYYFFME
                     LAKKMGLNDYPFVEKRTYLEKVIEPLKRFDKNLDIEKLKNNYFTIHNLVAWEDKKFET
                     PSGKYELYSESIKNLGISPTPVYISNKYKEIEDKNISFRLLTNHHADTLFSQHFMDKK
                     SIAQAYINQRMAKKVGIEDKDIVILRSKKAKINVQINIDDGVGNYIVKMYVGWWKKHG
                     NPNSLTDTGISDFGGQVTYNESMVEIIRQN"
     gene            2649..2984
                     /gene="spoIIAA"
                     /locus_tag="KPH29_002736"
     CDS             2649..2984
                     /gene="spoIIAA"
                     /locus_tag="KPH29_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HBG1468319.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            2998..3426
                     /locus_tag="KPH29_002737"
     CDS             2998..3426
                     /locus_tag="KPH29_002737"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HBG1468320.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            3449..4213
                     /gene="sigF"
                     /locus_tag="KPH29_002738"
     CDS             3449..4213
                     /gene="sigF"
                     /locus_tag="KPH29_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HBG1468321.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAVKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
     gene            4493..4930
                     /gene="spoVAC"
                     /locus_tag="KPH29_002739"
     CDS             4493..4930
                     /gene="spoVAC"
                     /locus_tag="KPH29_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HBG1468322.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
     gene            5084..6097
                     /gene="spoVAD"
                     /locus_tag="KPH29_002740"
     CDS             5084..6097
                     /gene="spoVAD"
                     /locus_tag="KPH29_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HBG1468323.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            6111..6473
                     /gene="spoVAE"
                     /locus_tag="KPH29_002741"
     CDS             6111..6473
                     /gene="spoVAE"
                     /locus_tag="KPH29_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HBG1468324.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            6656..7672
                     /locus_tag="KPH29_002742"
     CDS             6656..7672
                     /locus_tag="KPH29_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG1468325.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFVGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDRSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
     gene            8047..8889
                     /locus_tag="KPH29_002743"
     CDS             8047..8889
                     /locus_tag="KPH29_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBG1468326.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
     gene            8904..9368
                     /locus_tag="KPH29_002744"
     CDS             8904..9368
                     /locus_tag="KPH29_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468327.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIITCFGQMIALKTQAQDLLIGSISG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            9355..10527
                     /locus_tag="KPH29_002745"
     CDS             9355..10527
                     /locus_tag="KPH29_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG1468328.1"
                     /translation="MECFKEVVNMNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPE
                     LAFEEYKSQKALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPG
                     MGHACGHNIIGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFN
                     NVDAALILHPSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSV
                     NSMRLHLKDYARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKG
                     AAISTGTNVEIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGN
                     LTHEIPAIQAYIKLKENTATHTDEFAVAAGGEEGKVALIKAIKVLAMCGVDILYSK"
     gene            10571..11506
                     /locus_tag="KPH29_002746"
     CDS             10571..11506
                     /locus_tag="KPH29_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HBG1468329.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(12305..12703)
                     /locus_tag="KPH29_002747"
     CDS             complement(12305..12703)
                     /locus_tag="KPH29_002747"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBG1468330.1"
                     /translation="MKWKELYNNARGKLNPRTISPFIDAGGVAAAILTDSGNVYTGVC
                     IDTACTLGMCAERNAIANMITNGESKITKLVCVMSNGSVGSPCGACREYLMQLDKDSA
                     CIEILVNVETEKTVKLSELLPDWWGSDRFI"
     gene            complement(13002..15695)
                     /locus_tag="KPH29_002748"
     CDS             complement(13002..15695)
                     /locus_tag="KPH29_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBG1468331.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQST
                     ALSDSRGGYKFEENNKWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANQG
                     VYVEPSIFTTITTFDGQLLVKNAPEEHKVVDPEVAYVVTDMLESVITEGTGGVATLPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDNGGNNGGG
                     STTPPDNGGNNGGGSTTPPDNGGNNGGGSTTPPDGGGTPAT"
     gene            15909..16607
                     /gene="yunB"
                     /locus_tag="KPH29_002749"
     CDS             15909..16607
                     /gene="yunB"
                     /locus_tag="KPH29_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HBG1468332.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
BASE COUNT         6160 a         2159 c         2981 g         5388 t
ORIGIN      
        1 taataacaat tcaaaataaa taacttatgt aagaaccgta ggaactacgg ggatagcctg
       61 tggagaatta gtaagacata ttttagtatg caaaattctg tgaagcagga acactgtgac
      121 tttagtcatg ggaggttcag aaaatatagc tatgttttat atactgtagt tttgaacata
      181 gaaagtttaa taaattatag tatatattaa tttttaataa ggtaagaaag tagaaagaag
      241 gatattttat ggatataata aaaaaattaa gtcatggatg tacattagat tgtcatgatt
      301 gttgtaaatt caatgtttat actaaagaaa ataatgtagt aaaaattgaa ggagataaaa
      361 atcatcctta tacaaaaggt tttatatgca aaaaagggat ggctcattta gatagattaa
      421 atcataaaga tagaataaag actcctatgc ttaaagttga tggagtatgg aaagagattt
      481 cttttgataa ggctatagaa ataatggcag aaaagcttac atactataaa gaaaagtata
      541 catcaaagtc tgtcatgcat tatgaccaat atggaagtgg gtctgtgcta aaatacatag
      601 gagatatatt ttttaatttt tatggaggtg ttagcagaca taagggtggt ccctgttgga
      661 gtgcagggat gcatgctcaa aaatatgatt ttggagtagc taagagtcat gctatagaag
      721 atatgttaaa tagtaaaagt atatttgtgt ggggaaaaaa tccagcatat actacaattc
      781 atactatgca aataattaaa aaagctaaag aaaaagggat aaagatagta gtgattgacc
      841 ccatatatac aaaaacagct caaatagcag ataagtatgt acaggtaaat cctggaacag
      901 atggagcttt agctatagct atggcaaaaa ttatagtaga agataaatta tatgatgaag
      961 aatatataaa ctcgtatgtt attggttttg aagaatataa aaaatattta tcttcattgg
     1021 aattaagttt tttaattgat gaatgtggtg ttaaagagaa tgatataaga gagttagtgg
     1081 atttatatac taacaaatat tcaagtataa atgttggata tgggcttcaa aaatataaaa
     1141 atggtggaaa cacaataaga gcgatagatg ctttaggtgc tataactggt caaataggat
     1201 ttagtggtgg aggggtaaac tatgctaata aagtttatcc aagtgttata aattctgacc
     1261 cttacaatag ccaatcatat ggtgaagata gggagtttta tgtaagtaat atcagtaaat
     1321 ttatagaaga atctttaaaa aatacatcaa ataaagtaaa ttatgcatca gatgaacttg
     1381 atacaacatc aaataaagta aattacatat cagatgaact tgatacaaca tcaaataaag
     1441 taaattatgt atcagatgaa cttgatacaa catcaaataa agtaaactat gtatcagatg
     1501 aacttggtat ggcattacat aaagtaaatt gtgtatcaga tgaacttgat atgacatcaa
     1561 ataaagtaaa ttatgtatta gatgaacttg atataacatc aaataaaaca gattatatat
     1621 caaatgaatt gtataattta tctaataagt caatcaaaga taatattcct ataaagatgg
     1681 ctgtgataac taaaagtaat atgctgaatc aactgccaga tttagtagaa ttggagaggg
     1741 tgttttcaaa aattaaattt aaagtatgtt ttgatatgtt tatgactgat acagctactt
     1801 tatgtgacat gtttataccg tgtacaaata cactagagag tgaagatata atttatagct
     1861 caatgaccaa tccatatata acctataatg aaagagctgt aaagccagca cataaattga
     1921 tggatgaata ttatttcttt atggagctag ctaaaaaaat gggattaaat gattatcctt
     1981 ttgtagaaaa gagaacttat ttagaaaaag tgattgaacc acttaagcgt tttgacaaaa
     2041 atctggatat agaaaaactt aaaaacaatt actttactat acataacctt gttgcttggg
     2101 aagataagaa gtttgagact ccatctggaa aatacgagct ttattcagaa agtataaaaa
     2161 acttgggaat aagtccaaca cctgtatata taagcaataa atataaagaa atagaagata
     2221 aaaatatttc ttttagatta ttaactaatc atcatgcaga tactttattt agtcagcatt
     2281 ttatggacaa aaaatctata gctcaagcat atataaatca gagaatggct aaaaaagtag
     2341 gtattgagga caaagatatt gtgatattga ggtcaaaaaa ggcaaaaata aatgtacaaa
     2401 taaatataga tgatggagta ggtaattata tagtaaaaat gtatgttggt tggtggaaga
     2461 aacatggaaa tcctaatagt ttaacagaca ctggtatatc agattttgga ggtcaggtaa
     2521 catataatga aagtatggta gaaataatac gacaaaacta gttaaatttc attgaaggaa
     2581 taaaaatata attatagaat tgattaaaag ttactatttt tacaaaacta ttgaaggagg
     2641 gatttttgat ggtaaattat tccttagaac ataagaatct atacatagaa tttatgtgct
     2701 cagaactaga ccaccatgta gccaatgaaa taagggaaga aatagataac ttattaagtg
     2761 taaatcaagt aaaaaatgtt gtatttaatt ttgaaaatat taattttatg gattcttcag
     2821 gtataggcgt catcataggt agatataaaa agatttctaa tgaaggggga cgagtttcag
     2881 taattaatat aagttcacga gttaagaaga tttttgattt atctgggtta aataaaatta
     2941 ttggcattta tgatacgtat gaagaagctc taagttcttt gtagggagga gtatgtgatg
     3001 aataatatta tggaagttaa attttcggca atatctgaaa atgaaagttt agcaagagtt
     3061 atagtagcat cctttgcagc taagttagac ccaacagtag atgagttagt agatataaaa
     3121 actgctgtct ctgaggctgt tacgaatgct ataatacatg gatatgaaga agacagttct
     3181 aagtttgttt tcttaagatg tgagatagaa ggaaatacta taaagattat agttgaagat
     3241 gaagggtatg gaatagaaaa tgtggagaag gcaatggaac cactttatac atcaaaacca
     3301 gaacttgata gatcaggaat gggattcaca gtaatgaaaa gctttatgga cgatgtagag
     3361 gtaagttctg taaaagataa tgggactaga attgaaatga ctaaaaaaat aaatgtgcct
     3421 aaataaagtc caaaagtgag gttttattat ggaagtaact gttgccagag aagaaaaaaa
     3481 acctcttctt agtcatgaag agactctaga acttattgaa aaagttcaaa atggagatga
     3541 agaggcaaaa gaaatattaa tatcaagcaa tttaggtcta gttaggagcg ttgtatctag
     3601 atttttaaat ataggatatg atagagaaga tttatttcag ttaggttcta taggcttaat
     3661 aaaatcaata tataaatttg acccaaaatt taatgtaaaa ttctctactt atgcagtacc
     3721 aatgatttta ggtgaaataa agagatactt aagagatgat ggtatgataa aagtatcaag
     3781 gtcgttaaag caaatagctg taaaggcaaa gatggaaagt gaagctttaa ctaaaaaatt
     3841 aggtagagag ccaagtatag aagaattagc taaagcaata gatgtagaaa aagaagattt
     3901 agtaatggct atggaagcta atttcaatgt tgagtatttg caaggagtta ttcatgaaga
     3961 ggaaggttct ccaatatgcc ttattgataa aataagcatg aaaggagaaa gtgaagaaga
     4021 aaaagtagtg gataacatat tgttaaaaga catacttgga agactggata agagagagcg
     4081 tcaaataata gttctcagat actttgaaga catgactcaa agtgaaatag gagagatgct
     4141 caatatatca caagttcaag tatctaggat agagaagaaa gtattgtcta agttaaaaga
     4201 atatatatcg tgataatact tatgatatgt agaataacaa aatataataa atatattact
     4261 aaaataaaat gtaataactt tttatgttaa gtttttaaat tattaaaaga tactaaaata
     4321 ataaaaagca ttaaaaaagt aaaaatataa aaacaaggtg tctttatgga aaaatagatt
     4381 ttttctttat taggcatctt ttttattttt tgaccaaact ttagatagtc aaaataaata
     4441 ataggtttat ttttttaaaa ttaacaaata atactttaga gaggtgttta gtatggataa
     4501 aaattataaa aaatatgtag accaaataag cccaaaacct acatatttaa aaaactatac
     4561 acttgcattt attgtaggtg gaattatttg tatgataggg caagcaataa atgatttgta
     4621 tatgaaagtt ggaggtcttg ataaactagg tgctagttct gcaacatcaa taacattaat
     4681 atttataggg gcatttttaa ctggattagg agtttacgac ctaataggaa aaagagctgg
     4741 agctggttct ataattccaa taacaggttt tgctaactca atagtatctc cagcaatgga
     4801 gtataaaaga gaaggctatg tattaggtgt aggtgcaaac ttatttaaaa ttgcaggacc
     4861 agttttagtt tatggtatag gttcttctat tttatgtgga attatttatt atatttttaa
     4921 gatgttttag atgattttta tttaatttaa attttattaa ttatgaagaa ggtataaaat
     4981 ctgaaaatag ctactaaagt aactaattga aaaataattg tgcagattta attaatatta
     5041 aaaatttgaa tagtaaaatg caaaattaaa aaggtgtgaa aatatgaaaa ataaaagaat
     5101 tggaaaaaga acagtcaagt tagaaaataa accaaccata atatcaactg gaactatagt
     5161 gggtcctaag gaaggtgaag gaccactaaa ggactatttt gatatgatta tgactgacga
     5221 tttatatggt gaaaagactt gggaattagc tgaaagtaaa atggtagaga ctgcatccca
     5281 acaagctatc caaaaggcag gtaaaaaatt gtcagatgta aattatatgc taggtggcga
     5341 tttaataaat caaatagtgc cagcatcatt tgcagcaaga gaactggcta ttccattttt
     5401 aggtatatat ggagcttgtt ctacaatggc agaaggtctt tgtataggtt ctatgcttat
     5461 agatggtggt tttgctgatt tagtactgtc aggtacatca agtcactatt gtacagcaga
     5521 aagacaattt agattcccac ttgaacttgg aaatcaaaaa ccaatgacag ctcagtggac
     5581 agttacagga gcaggaagtg tattattagc tcctaatgga gacggtccaa aagtaaagta
     5641 tgttactgtt ggtaaagtaa tagatgaggg tattgatgat ggtaataata tgggaccagc
     5701 tatggctcca gcagcaatag atactatcta ctcttacttt gaagatacta atgatgaccc
     5761 aaatagcttt gatataattg ctactggaga tttaggtact cttggaaaac agatagcaga
     5821 agactttcta aaagaaaaag gtgtagatat atcaaaagtt tatactgact gtggtataga
     5881 gatgtttaac cttaaagaac aagatgtcca ctgtggtgga agtggatgtg ggtgttcagc
     5941 tacagtgttt gcaggatata tatatgataa gttgagaaaa aaagaattta acaaagtaat
     6001 gcttgtatca acaggagcac tactttcacc gacaagtaca ctacaaaagc agacaatacc
     6061 atgtgttgca catgcagtag taatagtaaa tgagtaggaa gtgatataat atgttaatgg
     6121 actatgtaag agtatttatt gtaggtggaa ttatatgttt aatagcccaa gtaatgatgg
     6181 attatttcaa aatgcaaact ccatatgtta tggtaacgta tgtaacaact ggtgtagttt
     6241 taacttttct aggactttat gagccattag ttaagtttgc agcagcaggt gcaacagttc
     6301 ctataatagg atttggatat tcacttgcta caggagtaat aaaatcaatt caaagtgatg
     6361 gttttttagg tattttttta ggaggtacaa ctgctacagc tggtggaata gcagcagcta
     6421 tattctttgg atatattatg tcagtagtat ttactccaaa agcaaaacca taaatcttta
     6481 ttaaaagaat aataagtttt tgaaataata actttttata aagcaggtgg atatctttta
     6541 gattccacct attttttata taaagaagcg tttacataat tgaagttgac aattatttac
     6601 aacatagtgt agtattatat tatcaaaata aaatgaagtg aagtagggga taaagatggt
     6661 aactattaaa gatattgcaa ggaatctagg tatcagttat tcaactgttt ctaggtgctt
     6721 aaacaataat cctaatgtat ctgaaaaaac aaaaaagaaa gtagtagaag aggcaaatag
     6781 actagggttc cattttaatg taaatgcaag aaatttagca aaaaaagaaa ctaacaggat
     6841 aggggtaatc ttttcaaata attttaatca tcaagatact agaaagtttt ttagtgatat
     6901 aatggatagt tctataaatt ctattgagac taataaatac gattttataa tacagcctaa
     6961 taataacatt agtggtgata gtaatgtata taaaatggta aatggacaaa tggttgatgg
     7021 attagtaatt gtgtctcaat caataaaaaa agaagagtat gattttttaa aagacaataa
     7081 ttttccacat gtatttatat attttaaacc atcttttgta ggagaagtag acaatttctt
     7141 ttgggacgat aacgtgtatg gaggttatat agctaccaag catttaattg accatggtca
     7201 taaagacata ataacagtaa cttcagatga taggagttca aaaatgcatg aagatagaac
     7261 taagggatat ttaaatgcta tggaagaggc aaatttaaag accgaagtca ttaaatctaa
     7321 aatggatttt gaatctcaag ttgaattttt agaaaaatat attgataaaa taaaaaaagc
     7381 ttcagctata tttgtacaac aagatgtacc tgcgatttct ataatacaag agttaaaaac
     7441 tacttatggt ataaatgtac cagaagatat ttccataata gggtataata atattgaatt
     7501 aatatcatat tttaggtcgc atttatcaac aatagatgac ccaagagagc aagttataaa
     7561 aaatggagta gatagtttag taaatataat aaataaaaag agtacagaac acattccaag
     7621 aaaattatat ccaaggttaa taatacgaaa tagcgttaaa agaataaagt agtaagcttt
     7681 taagtatcaa aagtttatta aaaatattat agattatgtg aagtttttta acaaacaata
     7741 taaataaaaa ttaaaaggag taggtgtata taagtacatt gtactccttt atttattgag
     7801 atagttttta taatatgaat gaataatttt attcaattat ttgaaaatcc gttattttaa
     7861 caatataaat taataaatta tttgtatttt atatcatgaa tctataatat ttttttgatt
     7921 cataactaaa agtatataaa aatgatgaat aaatatatat tatacattat caatgatagg
     7981 ttatataata aaatcactaa caattatatt ataagaaaat tataaaaatt acaaggaggt
     8041 atagaaatga aagaaaaagc agggattaga aatgccataa tagcagcctt tataatcaca
     8101 atattggcag aattaattgg acctatttca tttaaagtta tgggaataaa tgtaacttta
     8161 cttagtatac tatgggctat attcatagga atggctgttt ctcctcattt attagggaga
     8221 gttataccag ccttaaaaaa gtttataggg gataatgaaa taaatgtatc tccatattta
     8281 ttatctttga cactatatcc attaggaata atgtttggaa ttaatgcagg gcctaaagtt
     8341 ggtatattac ttcaagcagg accagcactt ttattacaag aatttggtca tatgggtaca
     8401 atgttgatag cattacctgt tggagtagca ttaggtcttg gaagaagtgc attaggtggt
     8461 actttttcat tatgtcgtga cacagcacta ggcattattg gagataagta tggattaaac
     8521 tcaccagaag gtataggaac actaggaact tacataagtg gtagtatatt tggaacatta
     8581 ttattttcat ttttagcacc agtgggtgta cttttaggat ttcatccata tgctttagca
     8641 atggcatcag gaatgggtag tggttcaatg atgggagctg caacagcatc attaatgaat
     8701 acggttccac aaatgtcaga gcagatttta gcatattcag ctactagtgg attgattaca
     8761 gctgttactg gaatatatat agaattattc ttatctttac cagtagccaa ctactattat
     8821 agcaaagtag ctccagttat tgaaagattt agaggaagaa aaacaagaag aacaagtaat
     8881 aaattgtaaa ggaggatata tatatggaaa aaacttttaa gactattatg gagcagataa
     8941 aaatagtatt gtttatatat attattactt gctttggaca aatgatagca cttaagactc
     9001 aggctcaaga tttgttgatt ggaagtatat ctggattttt gttggtgata attgctatta
     9061 taataaagaa ttttgttaaa aaacctaatt taccaggttt tgcatgggct acattagttg
     9121 cattcgtttt atcgcttcca atatctccag tgggggatat catagcaaca aatgtaggga
     9181 aaatcaattt tatgtctaca gtaacaccat tattagcttt tgcaggaatc tctgtaggta
     9241 accaattaga tatattaaag actttgagtt ggaagttagt agttatatca tttatagtta
     9301 tgacatctac ttactttggt tctgctttta tatctcaaac agttttaaga ttaaatggaa
     9361 tgctttaagg aggtagtaaa tatgaatgaa ttaaaagcaa aaattaaaga ggaaataaag
     9421 ttattatctg aagagaaaaa gacatcattt gaaaaagttt ctgactattt attttcaaat
     9481 ccagaattag catttgagga gtataagtca caaaaagcat tatgtgattt gttagaagaa
     9541 aatgaattta atgtaactaa aggtgtagga ggattagaaa catcttttga agctgtgtat
     9601 tcaaatggaa ctaatgggaa aacagttgct tttctagctg aatatgatgc acttccagga
     9661 atgggacatg catgtgggca caatatcata gggacaagtt ctgttggtgc aggtatcata
     9721 ttaaaagaaa tcatgaaaaa acataatata gaagggactg taaaggtttt tggaacacct
     9781 gcagaagaaa gagtaggtgg aaaaataact atgattaaag aaggtgtatt taataatgta
     9841 gatgctgcat taatcttaca tccaagtgat gcttcaatgc ctgatgatat atcatttgct
     9901 caagttaatt taaaatttga ttttactggt aaggcatcac atgctgctgc ttttccttgg
     9961 gaagggaaaa gtgccttaag tggagtaata gcactattta acagtgtaaa tagcatgaga
    10021 ttacatctta aagattatgc aagagtacat ggaattataa ctgatggagg tagcatacat
    10081 aatataattc ctgaaaaatc tacagctata tttaatgtaa gagccttaag catagaatat
    10141 ttaaatgaga tttgtgaaat gcttaaaaat tgtgctaaag gagctgcaat ttctactgga
    10201 actaatgtag aaatagttca attagatgaa atatacaaag agattaagaa tgattctgaa
    10261 ttagtgaata tagttagaca aaattttgaa gttttgggtg aagactatgt tgaaagagat
    10321 ttaagtcaag gtataggttc aactgataca ggaaatttaa ctcatgagat acctgcaata
    10381 caagcatata taaaattaaa agaaaatact gcaactcata cagatgaatt tgctgttgca
    10441 gctggtggtg aagaaggcaa agtagcactt ataaaagcaa ttaaggtgct agcaatgtgt
    10501 ggagtagata ttttgtatag taaataatat ataattttaa aatatattaa gaaaggaaat
    10561 cggattagac atgatattaa aacaagtaat agaggtttat gacattttag ataaagcaaa
    10621 tgctaatggg gaagaagtaa aaacttactt acaaggttat ggtgaagtag atgtaactgt
    10681 aaaagaattg tcttcaagta aaggttcaac agatttagta aagcttacaa ttcctgggaa
    10741 aaatggaaaa ttaaaaggtg gagatgctcc tactcttggt attttaggta gattaggagg
    10801 gattggagca agaccagaag ttataggttt tgtctcagat ggtgatggag cattggtagc
    10861 aattgcagtg gctgctaaac ttttggatat gcaacgaaaa ggggacatat tagatggtga
    10921 tgttgtaatt tcaactcata tttgcccaga tgctccaact aaagaacact atcctactcc
    10981 ttttatggat tctccaattg atatgatgac tatgaatgaa aatgaagttg atagtagttg
    11041 tgatgcaata ctttctatag atactactaa aggaaataga gtaatcaata ctagaggatt
    11101 tgcaatatcg ccaactgtta aagaaggcta catattaaaa actagtgata accttttaga
    11161 tattatgcag actgtaacag ggaaatctcc atttgtattc cctttgtcta ttcaagatat
    11221 aacaccatat ggaaacaatc tgtatcactt aaatagtatt ttacaaccag cagtagctac
    11281 agatgcacca atagtaggag tagcaattac aacagagatg cctgttgctg gatgtgctac
    11341 aggtgcaact aattttagtg atttagattc agctgggaga tttgcaattg aagttgccaa
    11401 gctatttggt agaaataagt gtgatttcta tgataaagaa gagtgggaaa tgcttattaa
    11461 gagatatggc aaattaaata gatttcaaac atttggtata cagtaattag aaaaatatta
    11521 agtttatata atttgtgata caactattta agaagatata aacaattagg aataaaagta
    11581 agaataaagc acttagttaa aaactttaaa aaaaaatatt tatttgttta tataaaaagg
    11641 aggattctgg ttattgggat tctctttttt atgaatacaa ataatatatt gtaattataa
    11701 aaaaaggagc gtttcaaagt gattttttta atcaccttga gacaactcct tatatttttt
    11761 atttaaattc tattttttat tgactgtata gctattttca ccatcataag gtggcatgac
    11821 cttgaacagc gtggttttgt aaagttgggc accacaaaac aaaaaatcaa caagcccttg
    11881 tgtggtctgc tgacgtgttt actatactat actttcccaa aaaccgcaag cactatggga
    11941 ggctataagg cggcgaccat tcaggggcga tcccctcgaa accgggatag ggttcccatg
    12001 tgggacataa taaagagcgc cccggaggta tcaactacca tccggggcgc tctttattat
    12061 gtcccgtggg aaccatgccc acgaagcagg gcgtacaacg gacagatagg tcctaccctc
    12121 ctgacagact ggaatctcaa tcccatcgaa aacaatcata acaatcatgg caagattata
    12181 tacactcctg taaaatattt ttgtgtgtac catgaagtac tgcttgccac tattgagaac
    12241 ggataaaagg gaagtacgta ttataccaat aaggtgaacg aaacaaaata ctcatttgat
    12301 aaagttaaat aaacctgtcg gaaccccacc aatccggcaa gagctcactc aatttaacag
    12361 ttttttctgt ttcgacatta actaaaatct caatacaagc actatcctta tcaagttgca
    12421 ttaaatattc acgacaagca ccacatggtg agccaacact cccattgctc atcacacaaa
    12481 ctaatttagt aattttactt tcgccatttg ttatcatatt ggcgatagca tttctttctg
    12541 cacacattcc caatgtacag gctgtatcaa tacacacgcc agtataaaca tttccgctgt
    12601 cggtaaggat tgctgcggca accccaccag catcgataaa aggtgaaatt gtgcgtggat
    12661 ttaattttcc cctagcatta ttataaagtt ccttccattt catcaggttg atcctcctgt
    12721 tgtatccata taaataatct tttatacgcc ttttccgcct aaggcacaca acacattact
    12781 cgcttttttc attctaatat tcgtgcgtga aaatgtcaaa cggtttactt cgtttcatta
    12841 cttcatactg ctgttcataa ccctgtttgt tcacttgggc accattcgag caccaattta
    12901 cccgtttcag ggttgtagcg aacacagcag aaatgctcat ttcatgggct tttctggcta
    12961 gaaaaaaaat tttatcgctg ccagatattt actatttttt attaagttgc tggagttcca
    13021 ccaccatcag gaggagtagt agagccacca ccattatttc caccattatc aggaggagta
    13081 gtagagccac caccattgtt tccgccatta tcaggaggag tagtagagcc accaccattg
    13141 tttccgccat tatcaggagg agtagtagag ccaccaccat tgtttccgcc attatcagga
    13201 ggagtagaac cgccaccatt atcacctgtg ttattattgt tattattatt ttcttctggt
    13261 tggttattgt ctggtttttt tgtatcttca gatgatggtt gagaacttac tttagtagga
    13321 gcagtacctt caataaatgc tgctttagag ccagaagatt gctttcctcc atcctctaaa
    13381 ttaatttttg taaaatatac atttttagga acttcaaatt cagtaaccgt taaattagca
    13441 tgtatttttt ccataatttt ttcccatagt tttgcagtac tagtacttcc atgtggtact
    13501 tctctacgtt ttatagtgtt accagaatca tcttttctac cagcatcatc tccaatataa
    13561 gttgcaccaa cataataagg agtgtaaccg acaaaccaag catcatatgc actatttgta
    13621 gtacctgttt tacctgctac aggcattccc tttggaagtg tagcaacacc accagtacct
    13681 tcagtaatta cgctttctaa catatctgta acaacataag ctacttcagg gtctacaact
    13741 ttatgttctt caggagcatt tttaactaat aattgaccat caaaggtagt tatagtagta
    13801 aaaatagaag gttctacata aacaccttga tttgccaatg taccataagc agcagccatt
    13861 tgaagtggag taataccatt cgccatacca cctagtgtca atgatgggaa ttttctatca
    13921 cttgcttcag atgaacctga gtgattattc tttaaagttg ttataccaaa gttctttaag
    13981 taatccatca ttatatctac acattcatca taagagtctc caagcatttc agcagttttg
    14041 acagctatcg tgtttgaaga tttagcaagt gctttacgta aactcataga accatgacca
    14101 gcagtagtag ttcttggatt ccatttatta ttttcttcaa acttatatcc tcctcgagaa
    14161 tcacttagag cagttgattg tgttattttt agggtatcaa tagcaggtgt atatattgct
    14221 aaaggtttta tagaagaacc tggctgatga gggttagtag cacgattcaa agtttttcta
    14281 ccacttatat ttcttcctcc agctagtgct cttatttcac catttttata atctaagatt
    14341 accattgcag cttgtggttg gctaactcca tcagggccga ccatatgtcc tgggaaattg
    14401 ctatttctat caaattcttc ttctaaagca tcttgcattt tagggtctat agttgtgttt
    14461 acaattagac caccattgta gaataagttt tgagcttcat catttgtatg tccttgttcc
    14521 attaaagcat ttataacttc actttcgata aggtcttcta cagatgaaga aactttatct
    14581 gatgattgag gcaattttat ttctatttta gctttgattg cttcatcata ttcactttgt
    14641 tttatatatt tcaattctaa cattttcttt aaaacaattt cttgtctttt tttagcttca
    14701 gggttactta cagcctttct aacaactaat gtacctgctt ttaattgtct ataagtatca
    14761 ttagatataa gctcccaaga ttttattttt tcaatcatat caaagtccac ttgagtaggg
    14821 tcatctaaat catcatcagt tgtgttaaca aaaaataata atttattttc taaatcctcc
    14881 ttagtttcat ttccctctaa cttagaagtt ttataggctg aaaatcttga tggattttta
    14941 gttgaaccag caagaagtgc tgactctgct agagtgaggt cagctgcaga cttatcaaaa
    15001 taaccacgtg caccagcctc agcacctgct agacctctac caacgaagaa gttatttaaa
    15061 taagtttcta gtattttatc tttacttact gttttactca tctcatgtgc atagtaaata
    15121 tcctttattt tacgaggtat agtttgctgt tcagttgtta acagcatttt ggatacctgc
    15181 ataggaatag tactaccacc ttgcttagta cctgtaaggg tctttaatac tgacctaaat
    15241 aaacctttaa tatcgacacc cttatgctca taaaaacgct catcttctat tgcaattaca
    15301 gcatgttgaa gataagggga aatcttatct aatggaacgg gtgtcttttt atttacactt
    15361 ggagctgtag acaatgtttt tccattagcg tatttaatag tagttgtttg gttcgtctgt
    15421 ttatctaaaa cagcttctgt aactggagtt acatctctta gtgacgcaaa tactagacca
    15481 gcagtacctg ctgtaccaac aactagtaaa actaaaaata ctatcccaaa tactcttagt
    15541 tttttaaatt tatcgctttt tttagatttt ttcttgtttt ttgttgaacg agtcttgtta
    15601 gctgaagttc tattaattgg tttatttgta ttaccagatg agctaacttt ttttctcctg
    15661 attttatttc catttttatc attatcgtta ttcataagat tttaacctcc ttacttaatg
    15721 caatattata acacaaatag gagtagtttt gtaattaata gaaaaaatga atttgctata
    15781 atattctgta cattgtatat gtataaacgt aatatattaa aaagtctata tttttatttt
    15841 atttattttt gacaaatatt ataaaaatta tgccaaagct acatataaat aaataggagg
    15901 ggataatttt gaatagaata gtagatagaa aaacaaaaaa tgaatttaga aaaatcatag
    15961 ctattttttt agtaattctt ttcttgtctg tatttatagg cagttttata tatatagata
    16021 aaactttaag accgacaata acagttttag cagagactaa agctcttgaa ctagcaaata
    16081 ggtcgataaa taaagctgtt gctgagatgg tagaaggaaa aataaattat gaagatttaa
    16141 tggatataca acttgataat aatggtaaaa taactatgat acaagcaaac actataatga
    16201 tgaatgaaat tgcatctgca atagctttag aaatacaaga tgaattaaaa aaggataaga
    16261 cagcaagttc ttacatacct ataggaacag cattaggaag tcctatatta gctaaatacg
    16321 gtcctaaact tgaagtgtct attgaaccta taggaacagt atcagtaaac tttaaaacgg
    16381 aatttgagtc ttcaggtata aatcaaacta gacatagaat atatttagaa gctcaaacac
    16441 aagttaaagt agtaatacca cttataactt caacaaaaca aattaaagct caaataccta
    16501 tttgtgaaac tataatagta ggtgatgttc cagaaagtta tgtaaatatt ccagaaaaaa
    16561 atctaggaaa tgttttacct aacactggaa agaataccaa caagtaatat aaagttgtat
    16621 atttttaaat aaattcaaaa taatagttta taaataaaat ttttggaaat aatattaata
    16681 taatttta
//
LOCUS       DAELXX010000090        16672 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.090, whole genome shotgun sequence.
ACCESSION   DAELXX010000090 DAELXX010000000
VERSION     DAELXX010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16672
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.090"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..309)
                     /locus_tag="KPH29_002750"
     CDS             complement(<1..309)
                     /locus_tag="KPH29_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468333.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     E"
     gene            complement(563..2263)
                     /locus_tag="KPH29_002751"
     CDS             complement(563..2263)
                     /locus_tag="KPH29_002751"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBG1468334.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(2674..3087)
                     /locus_tag="KPH29_002752"
     CDS             complement(2674..3087)
                     /locus_tag="KPH29_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBG1468335.1"
                     /translation="MILVSACLIGINCKYSGDNNENEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKIKSEKDL"
     gene            complement(3155..5533)
                     /locus_tag="KPH29_002753"
     CDS             complement(3155..5533)
                     /locus_tag="KPH29_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBG1468336.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKSEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSEISAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKPGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRNLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            5850..7301
                     /locus_tag="KPH29_002754"
     CDS             5850..7301
                     /locus_tag="KPH29_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HBG1468337.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKEVKGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(7505..10018)
                     /locus_tag="KPH29_002755"
     CDS             complement(7505..10018)
                     /locus_tag="KPH29_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG1468338.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGEINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNSYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTSDEQLEVLKSGKADVLLNVSMNPIEGVRTV
                     AKFSSKPFYFITPKGSTDLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETNYIKDTEKLKVGVLLNKPPFQNADKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLEDMAKKHEIDIVAGMIYDYEYSREHNIAMSRPFLSTQYTMMINNKLNENSLNGK
                     RIALAKGIDYYGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYEAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKSDKESENGNIENERDKIRREYNKKFFDVIKSQE
                     NGTTEMYSIMLDDTFHWIRITSKIIYNDRKIPMYVIGKIANIDSEKEEKNRLLDKVQR
                     DSLTNIYNAAYIRKLTTQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLVGDKVLIDV
                     AKVLDESFAKDDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNGESLS
                     ISMGVVITKRGQEYDELYQIADNALYDSKRQGRNRFKIV"
     gene            complement(10241..11062)
                     /locus_tag="KPH29_002756"
     CDS             complement(10241..11062)
                     /locus_tag="KPH29_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG1468339.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSIYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMIVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEMR"
     gene            complement(11581..12057)
                     /locus_tag="KPH29_002757"
     CDS             complement(11581..12057)
                     /locus_tag="KPH29_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HBG1468340.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVGNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            complement(12091..12669)
                     /locus_tag="KPH29_002758"
     CDS             complement(12091..12669)
                     /locus_tag="KPH29_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBG1468341.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGDEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(12773..15391)
                     /locus_tag="KPH29_002759"
     CDS             complement(12773..15391)
                     /locus_tag="KPH29_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBG1468342.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIETGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MNRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTVPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMASSISIDSSISIDSSISIDSSISMDLDTSSSLGIPKELMGSNTNNIDTLSNTSK
                     IDTLNKNHYNTAVTDKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFK
                     KKDLNIDCYLNAFNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGY
                     APMMVTEYCPMGVVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSK
                     VTCMLDNLYELEEIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKKATDLYKK
                     LDEIGTVAGHYYKGVE"
     gene            complement(15883..16566)
                     /locus_tag="KPH29_002760"
     CDS             complement(15883..16566)
                     /locus_tag="KPH29_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBG1468343.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAIIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
BASE COUNT         5204 a         2986 c         1803 g         6679 t
ORIGIN      
        1 ttcttctttt ttaaaataaa taacatactt ttttattagt ttaataacct catcttcgct
       61 tttttgaata ttagtatttg ataaaattag tggtagtaaa atatttaaaa aattgatatt
      121 tcctattttt tctccataaa atctatagta aataagttca tctctcagtc tattttcgtc
      181 tatatacaca catctttgcg ttttatcatc tattaaggat tttgtattta ttatagttaa
      241 atctttaaga gccctacttg cactactaaa agaagatgtg tagacttttt ttagtaattc
      301 gtcattcata aatatatcct ttctgaaaaa ttgaatgtaa ataaattgtt attaaaaaaa
      361 aagagacagc ccgtttaaga agctgtcata tataggggga tagtagtgta caaaaatcat
      421 tgtaatctac tatctatata ttattatacg atactacttt tttatatgta aagcatcagt
      481 tttcgacatc cctatatttt acagttatac aaattgcgta ctaagttcaa ttttattctt
      541 tttatttcaa cattttatca tgctacattc tttccggagc gtgcattccc aacaatttca
      601 gagaattttc taaagtttgt tttgttgcta aaactaatgc aagtcttgct tttcttattt
      661 ccaaattttc tactaaaatt ggattgtcat gataaaattt attaaacgct tgagctaaat
      721 ctaacataaa tctagtcact atatgtggct catttttctt tagagcaagt aaaatattct
      781 tattgaatga ttctataact ctaagaactt ctgcactatc ttcatcagct aatagactat
      841 aatctatatc agaagtcact tctacttctg cttttcttaa aactgcacag catctagcat
      901 gtgtatactg aacatatggt ccagtctcac catcaaaact aagcgttctt tcccaagaga
      961 aggtatagtc tttaattcta ctgttagata actcttggaa tactactgcc ccaacaccta
     1021 cttgcttaga tatttcatcc acatttttag catttggatt tttagctagt ataatttcct
     1081 tagttttatc tatagcttga tttaaagcat cttctaaaaa tactactctt ccttttctag
     1141 tcgacatagt accttcttca agtgcaacca taccaaatcc aacatgaatt aaatcttttg
     1201 cccaatcata tcccataagc tcaattacct taaaccattg ttggaaatgt aagttttgtt
     1261 gagaaccaac tacatatata cacttgtcaa aatcataagt ctcttttcta tatattgcag
     1321 cagctaaatc tcttgtcata tataacgttg aaccatcatt cttagtaata agtgctggtg
     1381 gcattttata ctcttctaaa tcaacaattc ttgctccttt agactctttt aaaagatttt
     1441 tctcttctaa tagttcgata actctgttca ttttatcaga ataaaaactc tctcctgcta
     1501 aagaatcaaa ttctatatct aaaagtttat acactctgtt aaactctttt aaactctcat
     1561 ttctaaacca ttgccataat tcttgtgctt cttcgtcacc attttctaat ttattaaacc
     1621 atgctcttgc ttcatcttcc atttctgggt gttgttcagc ttcatcatgg aatcttacgt
     1681 ataattttag taattctgga attggattag actctactac ttctttttct ccccactttt
     1741 taaaggccac tattaactta ccaaactgag ttccatagtc acctaaatga tttattctta
     1801 tagtcttgta tccttgcgaa tcatatatct tatataaagc attacctata actgtagttc
     1861 ttatgtgccc tatatggaaa ggttttgcta tatttggaga agaatattca actattacag
     1921 ttttatcttt tccaaaatca ctatgtccgt aattttcttt ttcatctaaa acttttttaa
     1981 ttacagtttc tgctaattga gatttattta caaagaaatt tacatatccg cctaattgta
     2041 ttacttttgt tatctctcca cttggttcta tactttccgc caattcactt gctatcatgt
     2101 ttggagcttt tctaaacact ttggctaatt taaagcatgg aaaagcatag tcccccatat
     2161 ccttatttgg tggaacttct ataagtgcct ctatctcttc tttcgataaa tcttctattt
     2221 tttctttaag gcaatttgat attgccacct taaaatcttg cattctgttg ctcctctcta
     2281 cattgttcct ggtttatatt gtgcttatct ctttatgtta aatcaaattt cgtgtttttt
     2341 caagtatttc attacagtgt aaaacacttt aataattatg ttttctgtat atattaaaaa
     2401 tactataatt gcagttttta atcacactat tattgatttt ttataagact tttttctatt
     2461 ttattgtttc ttgtactatc cagttgactt tatatacttt ttttaatctt ttatcaattt
     2521 tcatacatca atattaattt tatatacatt tattttgctc ttttatctat ttttatacat
     2581 caatattagc tttatatact ttttttactc ttttatctat ttttatacat caatattagc
     2641 tttatatact ttttttactc ttttatctat tttctataaa tctttttcac tcttaatttt
     2701 tattccttct tcttctaaca gccttgctgt tatacctttc cctggtatct ttttacctga
     2761 aaaactacca tcatatatta gattacatcc acaagagggg ctaccctctt ttagtattgc
     2821 ttcgctacat acatataaat ttgctatttt tagagtttca ttagcccctc ttacaaagtt
     2881 ttctgttaca tccttattat ctttagtaat tacttttcca tcaactatct cagcaggtgg
     2941 tcttggtgta gatagaccac ctaactgctc tgggcataca agtgtaaatt ctttgccttt
     3001 taaatattcc ttgacttttt cattttcatt attatcgcca ctatatttac aatttattcc
     3061 tattaaacat gcagatacca atatcataaa aatgtgctcc tatacaaatt cttaatatat
     3121 tcttatacaa attactaatg tattcttata caaattattt taatttaaca attgttactc
     3181 ctgctccacc ttctccatat tgaccacctc gttgtgattt tacatgtcta tttcttctta
     3241 aaatgtcttg tagccctgct tttaatactc ctgtgcctat tccatgtatt actgttacag
     3301 actctaaacc tgccacatat gcatcatcaa gatacttttc tacttccatt atagcttctt
     3361 ctaggtttaa ccctctaagg tcaacttcat tttttacact accagatttt gaccttataa
     3421 tgtttctcgt tgatttagtt acatttgttg aaacatcttt gcgtgttttt tgtaaagatt
     3481 taaaaggtag agtcatcttc attataccaa tttgcactac tgcttctttt ctctttttat
     3541 ctactgaaac tacactacca ttttgattta aagttattac cttaacttct tcacctggct
     3601 ttaagtcttt tatctcctta tttgatacct ttggaactat catacttttt actgttggct
     3661 gaagacttcc catagagctg gtcaattctt ttctaagttc ttctatctta cgattctttt
     3721 cttttgaagc tctttcttgt tcaagatttc taagttcttt tattataata tctacttctt
     3781 ctttagcttg tcttataatt gaaaatgctt ctgatttagc tttttcaatc atcttatctc
     3841 tttgactgac caatttttca agtttttcgt catactctac tttcagtttt tcaatctctt
     3901 cttttaatct ttcagcttct tctctatctt ctactgcttt gattctattc ttttcaacat
     3961 tctgtaacac atcttctaaa gctatgtttt ctgtatttat atattcttta gcacgtgaaa
     4021 tcacataatc acttagccca agttttctag aaatctcaaa cgcatttgat tttccgggaa
     4081 cacctataag taacttatat gttgggctta aagtttctat atcaaattca actgctgcat
     4141 tttctacacc tggtttagta agagcataat ttttaagctc actatagtga gtggtagcta
     4201 tacatttagc tcctacagaa tttatatctt ctaatactgc tattgcaagg gcagctcctt
     4261 caacagggtc tgttcctgct cctaactcgt caaatataac taaagagtct gctgttacgt
     4321 tttgtaatat agatactata ttcgtcatat gagatgaaaa tgttgataga ctctgttcaa
     4381 tactctgttc atctcctata tctgcaaata cattatcata tacacacata ctacttccat
     4441 aatcagcagg tatatgaagt ccactttgtg tcataagtgc aaatagacct acagttttta
     4501 ttgtaacagt cttaccacct gtgtttggac ctgttatcac caatgtatgg aagtctcttc
     4561 ctaaataaat tgtatttgct actaccttct ttttatccag cagtggatgt cttccatttt
     4621 ttatgtttaa atatttatcc tcatttaaag ttggttctat tcctctcatc tgtatagaaa
     4681 gttttccctt tgaaaatgca aagtccaatc ttccaagtat ctcttgatta gagattaaat
     4741 cttcgctcac ttctccaacc atagctgaaa tctctgataa aattctttct atttcctctt
     4801 gctctcctag tttcaactgc ctcaattcat tgttcatctc tactatagtc attggctcta
     4861 taaaaagtgt cgcaccagaa gatgactggt catgcactat accagctact tgcgacctat
     4921 attcagattt tacaggtact acaaatctat cacctcttag agaaattatt gcatcttgta
     4981 agtacttttg ataagtagtt gaacttatta ttgaatttaa ttttgaccta atggattgat
     5041 ttttttgggc tattcttctt ctgatatctc taagtgtact tgaagcattg tcagatattt
     5101 ctacttcact aattatagca ttgtatatct ggtcttctat gtccttgtac acatatagag
     5161 aatttgatag agattgtatt attggataat taaaatcttc ttcatcacta ctagataagc
     5221 tatttcttaa tcttctagcc actcttaagg tatctgctat cataatcaga cttcctgggt
     5281 caagagaagc acctatataa gctctcttta ccttatcttc tatatcatga ataccttcca
     5341 accctactga acctctcttt ataataattg attgagcttc actagtttct tctagcatag
     5401 atttcacttc tacaaaatca gtatttggta ctaaattttc tatatatttt agacctaaag
     5461 aagatgaggc tttcttcttt aacaaatcta ttattttatt gtattctaga actcttaacg
     5521 atttctcatt cattttctat attcaccatc ctcgatattt taactttttt atttaatttt
     5581 aaaaatgtat gttttcaagc atttccaata aaaaatgcaa gttttatttc tttttcattt
     5641 ctaaaattat tatatttttt ctcttttaga tatattttac acatattttg tattatacta
     5701 tgtatgtagc tatctatata ttatacataa acatgcttta tttgtaatat aatctgttaa
     5761 aaaatcttct ttaatatttt taaaggagtt tattttagct tataaaaaaa tagctactta
     5821 tatagataaa ttaaggagga acatgagtca tgggaaacgt attagaagga ttaaaaccag
     5881 aaagtgtttt taaaaacttt gagaaaatat ctcaaattcc tagaggttct ggaaatgaaa
     5941 aaggcataag tgatttttta cttagctttg gtaaaaattt aggtcttgaa actattcagg
     6001 atgaaagctt aaacataata ataagaaagc ctgctacaaa aggatatgaa aattgtccag
     6061 gtgtagttct tcaaggacat atggatatgg tttgcgaaaa agaaaagaat gttgaacatg
     6121 attttcttaa agaccctata aaattaagaa ttgatggtga catgatatat gctactggaa
     6181 ctactctagg agctgacaat ggtatagctg ttgctatggg tatggctata ttagaagata
     6241 atactttaga gcatcctgca ctagaagttt tagttactgt taatgaagaa gatggtatga
     6301 atggtgcaga tgcactagac cctagcttaa ttaaaggtca atatatccta aatatggact
     6361 ccgaggagga aggatactta ttagttagct gtgctggtgg taaaacttgt gttgttagtt
     6421 tacctgttga atacaaggaa gttaaaggtg acaaacaagg attattagtt gaggttactg
     6481 gactattagg tggacactct ggtatggaaa ttgttttaca aagagcaaat gctaacaaag
     6541 caatagctag agtattaagt gtattgaatg ttgattatga attagcttct gttgatggtg
     6601 gtactaagca caatgctata cctcgtgaag ctaaatgtgt aatagctgtt aataaagctg
     6661 atgttgaatc tgctaaaaaa caaataaatg acattttaac tgcatttaaa catgagttta
     6721 ctacttctga ccctggcatg acttactcag tagctgaaac ttctgtagac aaagttctta
     6781 ctaaagattg taaagaaaaa gttgttcaaa tgtcttgctt aactcctcat ggagttcaat
     6841 cagtaagtct tgatatagaa ggattagttg aaagttctac taacttcgct ataattgaaa
     6901 ctaaagaatc tactattgaa tttttaactt ctgttagaag ttctgttatg tctattagag
     6961 atgaaatcgc tgatagaata agacttttgg ctcaagcttt aggtgctaat tacgacctta
     7021 tagctcaata cccagcttgg gaatttaaaa aaggctcaaa attagaaaaa atatgcagtg
     7081 aaacttatga gaaattaact ggtaaagttc ctactgttat ggctctacat gctggtcttg
     7141 agtgtggatt attacttgat aaacttcctc atgctgaagc tatctctata ggaccagata
     7201 tgtttgatgt tcatactcca aatgaacatg ttagtatccc ttctgtagct aatgtttggg
     7261 attatgttat tgaaatatta aaatctatga accaatatta aatttaagtt ttgattctaa
     7321 atagatttta attttaagta atctatataa aattaactct gtaaattaat actactgtta
     7381 gtataatatt tactttcaat attaacttat agaaataaag cctaatgata gataataaca
     7441 tgtatcatta ggctttattg taatttaaat attattaaac tttattctgt tttaaaatta
     7501 attcttatac tattttaaat ctatttcttc cttgtctctt agaatcataa agagcattat
     7561 ctgctatttg atacagttca tcatactctt gaccacgctt tgttataact acccccatac
     7621 taatagataa actttctcca tttggcaact taatgagatg aatatgattg tatacatcat
     7681 tacatttatt gataagttct tccttattta ttatatcatt tatataaaca ctaaattcat
     7741 ctccacctaa acgacctact atatcatctt ttgcaaaact ttcatcaagt acctttgcaa
     7801 catcaatcag tactttatct cctaccaggt gaccataagt atcattaatt gacttaaaat
     7861 aatcaatatc tataatcaat aaagctccat ttttttcagt tgataaattt aataaatttt
     7921 gtgtagttaa ttttctaata tatgctgcat tataaatatt tgttaaacta tccctttgaa
     7981 ctttatccaa caatctattc ttttcctctt tttcagaatc aatattagct attttaccaa
     8041 ttacatacat tggtattttt ctatcattat aaattatttt tgaagtaatt ctaatccaat
     8101 gaaaagtatc atcaagcatg atagaataca tttcagtagt cccattttct tgtgatttta
     8161 ttacatcaaa aaacttttta ttatattctc ttcttatttt atctctttca ttttctatat
     8221 tgccattttc actttcctta tcacttttat ccaaaccata ataagtctca ataccctttt
     8281 ctggcataga aattatcaat ttatcttgct tataatcata ctcaaaaaag tattcacttg
     8341 tcatttcata caactgagta tgttttttga gttccaaaga tatcttttcg ttcatacttg
     8401 tacgagtatg taaaccaaat gccataaatc caataataca aattaataaa cttgctacaa
     8461 taaaaatagt ctcaattgga ttctgttgca taatataaag caacgagaaa tcttgtctat
     8521 atgtagtatt ttggtagaca attgattgca ttttttctga tgaaatactt ataatagctt
     8581 tattaaatat attcaataat tcttgtttat cttttttaat tatgccaaaa caaatactct
     8641 ttgcttcata agtttgagga actaggcgca catttttata cttaggttga ttcatatagt
     8701 actgcataga ataaccttct ccataagtgt aatctgcata gcctttatta acagcttcta
     8761 tacactcttc taaattatca taccaaacta tattttcttc aaactgacca taataatcaa
     8821 tacctttggc tagagctata cgctttccat ttagactatt ctcatttaat ttattattaa
     8881 tcatcatagt atattgagta gaaagaaaag gccgagacat agctatatta tgttcacgag
     8941 agtactcata atcataaatc attcctgcta caatatcaat ttcatgtttt ttagccatat
     9001 cttccaaatc ttcttgagat tttacaggca caagttcaaa ttccaaacct gttttatcac
     9061 atatatgatt taacaaatca attgatatac ctcttaattc tcctgtatct ttatctgcat
     9121 tttgaaacgg tggtttgttt aaaagtactc ctacttttaa tttttctgta tctttaatgt
     9181 aattagtttc ttcatcagat aaaaacattt gattattctc tggcgtaaag tatttctcat
     9241 ataaagtagt tgaaaaatat gggtccttct gttcaatatt attaattgct gaattaagct
     9301 tttgagttaa gtctgtactg ccttttggag taatgaaata aaatggcttt gatgagaatt
     9361 ttgctacagt acgcacacct tctattgggt tcatactaac attaagaagt acatctgctt
     9421 taccactctt tagtacttct aattgctcgt cacttgtttt acaatatacc aattcaggtg
     9481 acataagatt cattttacaa tactcatcta attctttaag cctagtatca gaattttcta
     9541 ttacagcaat acgaattttt ttcacatctt gcgaactcat tatgttatca atatctgtat
     9601 tttcatataa tgcttgtagt actgtatttg ctactccata actgtttccc gcataattat
     9661 atattttttg cattggctca ttgtataaca tacctcccat tatatcaatt tcaccatttt
     9721 ctaacatctt cattaatttt gtaatagatt catttatctc tccaggaact tgaacaaatt
     9781 cataattcca tccagtatac tgtgcaattt ctagcaaata ttcatatgta tacccaaaat
     9841 agttaccttg ctcatctatt tcagttaaac ctttttgaat aggataagca acacgaatgg
     9901 tacgattatc attacctata gcattgatac caaaagaaaa tatgcaagaa gtgaatatag
     9961 atgtaattac aataattaat aaaagtaatt tttttaaatt cactacacaa tcctccatat
    10021 agttaagatt tacagtataa attactgcat aaacttgtag acatgaaaaa atagattagt
    10081 atataaaagg atatataaat ctacttagaa atttaaatat ccttaatata tacaatatat
    10141 ttaaattata actcatttat atatctatta caacaaattt accctgctaa atacctactc
    10201 tatatcccat aattttacaa gttgtaatga agtttaagtt ctatctcatt tcaaatttag
    10261 cagtgtttct atttacagtt cttttatatt tcacatctgg ataacccaat ataaatgaag
    10321 tgataactgc ttcgttctcg ctcattccta acagttcttt tatctttggc tctacatttg
    10381 atgccataac aaaaaatcca ttataacaaa ttcctaatcc ttgagaatag gccataagtt
    10441 ccatattaga tgcagccaat cctccatcta cataagcact tccacctaga gacatatcac
    10501 ctacaactat cataagtagt ggggcatcat aaaataaaga atcatttccg ttctctttat
    10561 atcttttgta cattttctta aatgtatttt tatagataga acgtacaggg tcattgtcat
    10621 cagtatctaa tgccaaatta taaagacctt gtattgccag ttctttcact tcttttagct
    10681 tttcttttac taatatgtat cttataggct gtctattacc acctgttggt gtgtatctac
    10741 cagcttccac tatgtttttt attttttctt cttctacctc tatatcttta tattgtctta
    10801 tacttcttct aaatttaata aaattcaata acttttcggg ttctaattca aaactttcac
    10861 gattgtattc tattacttca tttttatcaa atccttgcat ttctattgca tctgttgggc
    10921 aaattgccat acagtgacca cagttaatac agagaatatt tttaaccttt gctttatcat
    10981 ccttaacttt tattgcattt acaagacagt ctgattcaca catcccacag cctacacatt
    11041 tttctaaatt tatgtctatc attccaaatt attcctcctt ttaaaaaatt tactttagta
    11101 taaaacaaat agttgtctaa gtcaattttt atttttttaa caatatacta gccttaacta
    11161 cactttaatg gagaaaaata tacataaaat tagaacttaa attagaattt attcaaaata
    11221 aaagataaaa gaagtaaatg tctaaaagtg aactttttag ttaatgagac attctttatt
    11281 gtatgaaatc aagtggatat ttatcatttc aacaataaaa aaggcttacc tctattttgg
    11341 atatgtcctt gttaagtaga cagactaaaa aaggctatct ttatgcactg agatagcccc
    11401 ttttcccaac tactattgtt tattttaata ttatatcaat aaatttaatt tgctataacc
    11461 tgtaagttac attttaattt gatgtaactt taaattacat ttttaagtaa tatctttaaa
    11521 ttatattctt aaaatatatc attaaactac attttaatta atctaaaaac tatatcttaa
    11581 ttaatctaag aaacttacat tataatcttt tagtttccca ttatcatata caatctttgc
    11641 actaaagata tttccagtct tagcccaatt taagaacaac ctgtcacctt cccacatatt
    11701 taaatcgaat attttatcat taccaaccca aattaaattt ccttctttac aatcatttaa
    11761 atctttttca gacaattcac cttcaaactc atctgagaaa tacaaaaaca tataccaatc
    11821 ttcttcatct ttaaattttg gaaaagttat caacccttta tatgacaatg atttaagagt
    11881 aagaccagtc tcttccttta cttccctaat tacacattct tctggagtct ctccttgctc
    11941 aaactttcct ccaactccta catacttacc ttcatgtata tcatctttct ttttatttct
    12001 gtacaacatc aaagtcttgt catctttttc tatataacaa atagttgtaa gtttcatgta
    12061 tctcgcctta ctttctcttt atattctcaa ttaattattt tcagcacttt caagcacatc
    12121 acttatattc ttcattgcat taagagaaag ttctgcaaat tcatcaaatg gaatccctat
    12181 atcttctata cttctcattg catctctatt tacatttgaa gcaaatctcg tttctttcat
    12241 tcttttaata acagacttag tctttacact agtaagtttt ttatctggat aaactaaagc
    12301 tatagcagtt ataaacccag ttatagggtc tgctgcatat attgcttttt ccattaagct
    12361 agttcttgta actcccattc catcatgatg accaagtatt gctctataca tttcttcgtc
    12421 accgaagttt tcttttttaa gcagttctac tgttgttgta gcatgtcctt cacatatttc
    12481 tcctggcttt cttccaacta tatctgaatc caaatcatgt aaaagacctg ccactgccca
    12541 ctcttcttct ttttctggct ctagtctttt ggctaattct ctcataacag cttcaacagc
    12601 ataacaatgt tttctcattc tatcagtttc taaatactta aataaaattt ctaaagcttc
    12661 ttctctcatt ttcaccaatc cccctgtttt ttcacatata tgatattttt atatattaat
    12721 acacataaat tcaagtcttt atatatattc atgtatattt ttcatgttat atctattcta
    12781 cacctttata gtagtgtcct gcaactgttc caatttcatc taatttctta tacaaatctg
    12841 tagccttttt tccaagttta taattattac tcaatacctc aatgtgagct tcaataacct
    12901 ttttaaccat ctcactatct tcaactgtaa aatctatcct aaatacatct attccaattt
    12961 cctctaactc atatagattg tccaacatac aagtaacctt cgaattgtaa attgtacttc
    13021 tacagaatat atcttgtgtt atcctataac ttgcatccgt aaagtctctt aaagcataac
    13081 tactttcctt acatttagca actctcttgt cctttttaca atctctaaca actactccca
    13141 taggacagta ttctgtaacc atcataggag catatccata tattactgct tcaatttcta
    13201 catctgtata ttttaaagtt tgtttaattt catttaagtt tagttcttgt gacaaacaaa
    13261 ctgtacttgc tccctcttct ttgaagtggt ttatactttc actattgaat gcatttaagt
    13321 agcaatctat atttaagtct tttttcttaa agtattccat agagccccag ttaccaattt
    13381 gaaccttatt aattcctttt atattatcca atatctcaaa ttgcttgtac tctttatttc
    13441 taacaatcct tggtgctgaa tagattaatt ttttgtcagt gactgctgta ttatagtggt
    13501 tcttattcaa agtatctatt ttacttgtat ttgacaaagt atctatgtta tttgtatttg
    13561 aacccattag ttctttagga atacctaaag acgaagaagt atctaaatcc atagaaatac
    13621 ttgaatctat agaaatactt gaatctatag aaatacttga atctatagaa atacttgagg
    13681 ccatttgtaa agcttttgat aatgtattta tatcttcata atagattaaa tctacattat
    13741 aatttaatac tgcttctaat tgctccaagt tcttaacttt aactctaatc ttaggaactg
    13801 tatcaagttt tttctgttta tttactctat ttggttggta ttttaatcta ttcttcttaa
    13861 attttctatc ttttatcttt attctttctt catctaaaag ctcaatacaa tcccttctca
    13921 tttgatttaa cacacttata ggcatactta caccttcatc caggtcaatt tctatctctt
    13981 ttaacttata tggtgtattt ccaagctttt gaatttgtgt ttctattttt tctttactta
    14041 aagccacctt tatagctttt tcaactattt tgtctccttc taccaccgat ttatttccac
    14101 aaaaatcact tataaaaagc tctggctttt tacctagttt aatactcact tttgcacgta
    14161 tattagtttt tatgaactct ttatcttgct gaaatgtagc ttgtactcta ttcattaatt
    14221 cgctgtcaga agtcttatat ataagctgat ttctctttgc ttctccaaca aaatctaatt
    14281 ctatcaaatc ccctttatgt gctattgtag ttatctctcc atcctttatt attcttccaa
    14341 tagtacctcc acctaagtta attccatcac ctttcttaag cgtattttct agcattactc
    14401 taagtctttt gttctttcta ttataatcta aaacttttcc aatatatagc ccttgattat
    14461 taggtctatt agagttcatt atatccttac caacttcacc taatatatat ccttttgtaa
    14521 actttctatt aaatatggta tacaaattat ttattatttc ctcagaaata cttatttttt
    14581 tcttatctac aaattcatta atcgcttctc tatatcctga aaccacagta gctacatact
    14641 ctggtttttt cattctccct tcaattttta aagataatac acctgtttca attattttat
    14701 ccaaatcttc tatagtactt aaatctctag gactcaacaa ataatctcca ttacttttta
    14761 ctacttctcc tgtatttata tctatcaact catacttttg tctacatggc tgtgcacacc
    14821 tacctctatt tccagaccta ttgcctatca tactactcat aaggcattga cccgagtaac
    14881 atacacataa agctccatgt acaaaaactt ctatatctac agttgtattt tcacaaatcc
    14941 attttatctc atcaacattt aattctcttg ctaaaacaac tctatcaaac cctacactct
    15001 gaaggtattt tacatcttct aaggaatgtg ctaccatttg agtgcttgca tgtagttcaa
    15061 agtcaggtaa tagttttttt ataagtcttg ccattcctat atcctgtact attaaagcat
    15121 caatatctat atcgtataaa aattttacat actctacaaa atcttctatt tcatactgtt
    15181 ttatcaatgt atttacagta acaaagactt gcactcccct tatatgagag tatttaacag
    15241 cctcaatcag ctcatttcta tcaaagttat ttgcagatgc tctagcacta aagtctttac
    15301 cacctaaata cacagcattt gctccgtttt gaactgctgc ttttaatgaa tcaaaagagc
    15361 ctactggtgc aagtaattct atttttttca tatttttctc ctctatttat acttgttagg
    15421 ttaatataat tatatctata acttaaatat ttgtattaaa tttatatatt tcttactata
    15481 ttttaaattc ttcaattaat ttatatatta gttatttata tttggtccat ttgagttaac
    15541 atatacatta tttactatat ttagtatatt tctattaact tatatattga ttactgtatt
    15601 ttaaattctt caattaattt atatactgct tactaaattt aaaacatttt aattaatttg
    15661 tacactgttt actatacttg aaatacttct attaatctat atattgccta ctatacttaa
    15721 gacattttaa ttaatttata cactgcttac tatacttaaa atacttctat taacttatat
    15781 attgcttact atattttaaa ttctttaatt aatctataca atatttgcta tatataaagt
    15841 atatcaaata acagcttcat ttttttgtaa atttacagaa agttaatttt cattaaatga
    15901 atcatcttta ttgataaaca taattccaaa gaccactgcc aaaataaaaa aacatattag
    15961 tattgatttt tccacgaaaa tttttgtaaa aaaagttcct aaaatagctc cagatataaa
    16021 gaatataata atagcataat actgtaaact cttttttatc ataaaaatat tcttagtatg
    16081 tatataatga aataaagttt ctgtagcact tcttaaattt ccagtacaca ttgtcgttgc
    16141 aaaactattc ccatttactt ttctaaaact ctctacttgc attgaacaaa taaaagaaat
    16201 agcaacattt acaaacatat taaatcttcc tctaggaata aatgaaacaa taagtattgt
    16261 aatcatttca attataatga taacctgacg ccaatgaatc tttgtcttat tatttgtaaa
    16321 atactttctt atcttctcgg ctataaatac acctaacata aatgccaaaa taggaatcaa
    16381 ataataaaat gattccttta cattaccttc tgctatatta agcccaaata aaactatatt
    16441 tccagtttgt gcattagcaa agactttacc tcttaccaca taagtataaa tatccaaaaa
    16501 tcctcctgtt attgccaaca aagaacataa aaagaatgat tcagacatct gactcttgct
    16561 tgtcatatat aaacctctcg ctttctaatg atagatacaa aatcaaaatc attttagatt
    16621 ctatttttca ttgatataaa aaaattattt atatttaaat attatctata tt
//
LOCUS       DAELXX010000091        15957 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.091, whole genome shotgun sequence.
ACCESSION   DAELXX010000091 DAELXX010000000
VERSION     DAELXX010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15957
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.091"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            156..698
                     /locus_tag="KPH29_002761"
     CDS             156..698
                     /locus_tag="KPH29_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG1468344.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGT
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
     gene            1023..2516
                     /locus_tag="KPH29_002762"
     CDS             1023..2516
                     /locus_tag="KPH29_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG1468345.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLGYNIPSRVQREVIPKLLKGQNLV
                     VRSKTGSGKTVSFAIPLCENINVDCNNIQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQSIKDQIAELKQRVHIVVATPGRILDHINKGSIKLENVKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVSLFSATIDEEIKYICEKYMLDYSVINIEENESDTNQKTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNILVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIGYKVNELKDIEKSRITHGKILFEEYSKG
                     ILKKVSKRKKVDKNSYKSKDIKLNIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
     gene            2753..3517
                     /locus_tag="KPH29_002763"
     CDS             2753..3517
                     /locus_tag="KPH29_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG1468346.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIIDFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(3802..6981)
                     /locus_tag="KPH29_002764"
     CDS             complement(3802..6981)
                     /locus_tag="KPH29_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG1468347.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTNQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKRDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGIDKMYILKNITEFAYSKLNGYELDFGKKFTYNPNIHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQILSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DGNICCDVKYIYDDEDKKFVLPNIQKETTIENKLTSSQFTKENNHYVFKGTDYDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPKEYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMDKSKNNKIYKNKALYIN
                     DVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPANLNANLRDYQINGLNWFKVLDY
                     YKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIKVLL
                     IHGNKRDREKCFMELENFDVILTTYGTLRNDLEKYSEIKFDYCILDEAQNIKNPVALV
                     TESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDNVKN
                     LKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKMKDKNLK
                     KDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSINENHKILLFSQFT
                     SVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNNSMDKKVFLISLKAGGTGLNL
                     TSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQESKKE
                     LINQFINGELSNEGVLKSLSDEEIIDLFN"
     gene            7262..9439
                     /locus_tag="KPH29_002765"
     CDS             7262..9439
                     /locus_tag="KPH29_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG1468348.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYRQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKDLIDREYIE
                     KHVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFPNSGRLFKALHSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANVMNIDDDLLKAGYEASIRHMFKKTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDNEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEASCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYRESDINSIIDDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            9453..9875
                     /locus_tag="KPH29_002766"
     CDS             9453..9875
                     /locus_tag="KPH29_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG1468349.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGACPKNCFKKEEGFVVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            10282..11085
                     /locus_tag="KPH29_002767"
     CDS             10282..11085
                     /locus_tag="KPH29_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG1468350.1"
                     /translation="MKKLKLIIMLALVLALLITGCSSGNNKNKEEQKTIRVATSGTYY
                     PFAFKDKDKLKGFEVDFWDEFAKRTNCKVEWVLTDFSGLFGLLEADKADVVSAQLTAT
                     PEREKKYAFSDAYNYSGTNIIVREDDTSIKSIDDLVGKKVGVGTGAVANEILKEKYPN
                     DEIKIVNYSSATLKGNYQDLEMGRLDAVVAQDVEALIAIKEQKLNLKMVEPPIQFGAC
                     SFAIQKNEKGEKWTKEINEVIEEMHKDGTLTKISEAWLGKDITKEPINE"
     gene            11078..11746
                     /locus_tag="KPH29_002768"
     CDS             11078..11746
                     /locus_tag="KPH29_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG1468351.1"
                     /translation="MSNFNFEFCLSIFPFLIKALGITILISIVAFFLSILLGVILAFF
                     TQTKSKVLQYITRVYVSFFRGTPLLIQIFFFYFGFPMIFSFMKDCSPYLAIVICLGVN
                     SSAYMTEIIRASIESVDKGQQEACYAFGLSKYQSMVKIILPQAATSAVPPIVSCTLDI
                     IKGSSLGLTIGIQDLMGVAQVEAASSFKYFEIYMITILIYWAVSIILSNIQSKVEKRI
                     GKAY"
     gene            11982..12644
                     /locus_tag="KPH29_002769"
     CDS             11982..12644
                     /locus_tag="KPH29_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG1468352.1"
                     /translation="MIFNMKYFLELFPIIMKSFNVTVILAISSLVFSLIIGTIVALSS
                     YYKVKVLNQICKVYVSIFRGTPLMPQLFFLYFGLAYMNDFVKNMDPVFATSIVLSLNM
                     GAYMSETIRASILSIDKGQIEAAYVMGLTNLQTMKRIVIPQAVRVALPSLFNNFIDLI
                     KGSSVAFVVGVNDIMGAAKSQGALSFKFFEVYGAVMVIYWCIITPLGWGQKKLEKKIS
                     NY"
     gene            12704..13501
                     /locus_tag="KPH29_002770"
     CDS             12704..13501
                     /locus_tag="KPH29_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG1468353.1"
                     /translation="MVKRNIIKCLVAVLSLTFVVAGCSKKEEVKDEKVITVASSNSAD
                     PYSYVDNGEHKGFEVDMWKEIGKRSGYKIKMEPTGFSSIFGMLDSGKVDVAANFFGMS
                     KERLEKYDASIPYGSDSVCIAVKDGNNEINKLEDLKGKVVAVSEGSQGQQVAASVNKD
                     NLFEEKVYGDGTNGLQDLDLGRVSAWIDAELTVVGDAKKANMKVRVLDEKLSVTNIAY
                     FFKKNDEKSKTIKEDVNKAIEEMLSDGTVKKISEKWFGMDVSADIQK"
     gene            13634..14809
                     /locus_tag="KPH29_002771"
     CDS             13634..14809
                     /locus_tag="KPH29_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG1468354.1"
                     /translation="MIETKIKSIALKNREKIIKIRRQIHSNPELAFKEFKTSKLIKEE
                     LNKLKIEYIDVAGTGVLATIKGKNNGGKTILLRADMDALPIKEENDLEFKSINDNMHA
                     CGHDAHVSWLLGTAMILNDIKEELNGNVKLLFQPGEEKGGSDIVIKENVLEGVDALAT
                     GHCWPTIESGKIGIARNCAMAATNTFEITIIGKGGHGAEPHNCIDPIAVGNAVYSSIQ
                     QIVSRKIDPVVPVVVSVCSFNSGVSKNIIPDVCTLQGTIRAISKEQVIEVSKILENIV
                     RNVCRAYGADCKFEKSTGGDAVINDKNMIELGRESACKILGVENVEIIDFPAMTGEDF
                     AIYMKEKPGLFMYIGVGNKEKNINYRLHSNKFNIDEKCLSIASSLFSQLAVDYLCQL"
     gene            14918..15667
                     /locus_tag="KPH29_002772"
     CDS             14918..15667
                     /locus_tag="KPH29_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBG1468355.1"
                     /translation="MIKVENLHKSFKGTEVLKGINLEINQSEVVAILGPSGTGKSTLL
                     RCLNYLVEPTKGKIMIGDVKVDVEKATKKDIANLRKHTSMVFQNYNLFKNKTALENVM
                     EALIVVHKKSNKEAREISLKLIDKVGMLDRKDFYPSKLSGGQQQRIGIARAMAVNPNV
                     ILFDEPTSALDPELVGEVLSVIKSLAKEGITMLIVTHEINFAKEVADKIIFMDNGVIA
                     EEGTPDEIFNNPKNERTAKFLNIVRKEEVFS"
BASE COUNT         6228 a         1679 c         2612 g         5438 t
ORIGIN      
        1 atatagactt atgataaaat aagacaaata tgttaaaaaa atgataatca cggagaactt
       61 ttaccttctt caattaatat attattatta gtaataactt taaaattata ttactattat
      121 ataaaaattg gcttaaaaga aggtgaaaaa agtaaatgaa aatatgcata acagtaggtc
      181 atagtatact aaaaggtggt aaaagtacag gtgtaaatgg tatagttgat gaatacaatt
      241 ataataaaaa attagctcct atgttagctg agatgcttat aagtcaaggt aatacagtag
      301 atgtaataat ttgtccagaa aggcacttta tgtcagaaag agaagaattt ttctatagag
      361 ttccaaaagt aaacagtggt aaatacgatt tattagtaga gctacattta aataaagctg
      421 atggaacaca atgtggtaca gaagtgcttt actatggcac tgaaggatta gaatatgcta
      481 aaagagtatc taatagactt ggagaattat ttgaaaatag aggagctaaa aaaagagaaa
      541 atttatatat attgagaaat acaaatccag ttgcaataca gattgaaagt tttttctgtg
      601 ataatgtgaa tgattgtaac aaagcaaatg aatcaggtta tgattatata gcacgattga
      661 ttacagaagg tatattgaat aaagatataa atatgtgaga ttatggtgat ttaggtattg
      721 attaatacct tttaatatta gatataaata aaaatacaat gtattagtat aatttaaact
      781 ttcaaaatat agattagttt gattattgac aagagatacc tcctaaatat tgatttatca
      841 gtatatttaa ggaggtattt aaatataaaa atttaattga tttgctacta aactataggt
      901 ttatagagta tatattttta tcttcagtct aataaataat ataaaaatag tagaaccttg
      961 tattataata atataatgta gaaaaatata tagataagat aaatataata aggtggtaat
     1021 atatgaaata tacatttgaa aaatttaagt taaatgaaaa gatacttaag tcattgaaga
     1081 gtttaggata taatatacct tctagagtac aaagagaagt tattccaaaa ttattaaaag
     1141 gtcaaaactt agtagttagg tcaaaaacag gaagtggaaa gacagtaagt tttgcaatac
     1201 ctttatgcga gaatataaat gtagattgca ataatattca agctcttata gtggttccaa
     1261 caagagaatt agccttgcaa gtaaaagatg agatatcaga tatagggaga ctaaagaagg
     1321 tgagatgtag tgctatattt ggcaaacaat ctataaaaga ccaaatagct gaattgaaac
     1381 aaagggttca tattgttgta gctactccag gcagaatact tgaccatata aataaaggtt
     1441 ctattaagtt ggaaaatgta aagtatttag taatagatga ggcagataaa atgtttaata
     1501 aaggttttgt tgaacaaatg gaaaaaatat tattaaattt acctaaggag aaaatagtaa
     1561 gtcttttttc agctacaata gatgaagaga taaaatatat atgtgaaaaa tatatgctag
     1621 attacagtgt tataaatata gaagaaaatg agagcgatac taatcaaaaa acaagacaaa
     1681 tagatgacaa aataatcaag gcaaatggaa gagagaagta catcctttta aaagaattaa
     1741 tatacagcga aaatccaaag agtgtgataa tattttgtaa taccaaagag aaagtatcta
     1801 aattatataa taagatgagt aaagagggtt ttttaataag agagttacat gcagatttat
     1861 cacaagaaag aagaattttt gtaattaagg attttaaaaa ccaaaaattt aatatattag
     1921 ttagtagtga tgtagcgtcc agaggaatac atattgatga tatttcttta gtaataaatt
     1981 atgatgtgcc acaagataaa gaaaactata tacatagaat aggtagaact gggagaaaag
     2041 gaaatagtgg aaaggcaatt actatagtaa ctgaaaagga tgaaaaatat atagagaata
     2101 tagaaacgta tataggatat aaagtaaatg aattaaaaga tatagaaaaa agtagaatta
     2161 cacatggcaa aattttgttt gaagaatatt caaaaggaat cctaaaaaaa gtatcaaaga
     2221 gaaaaaaagt tgataaaaat tcttacaaaa gtaaagatat aaaactaaat atagaaagtg
     2281 aagttgttaa attatattta aatgctggta agaagaagaa aataagagtt ttagatatag
     2341 ttggtgcatt tagtaatata aaaggtataa ctaatgatga tataggtgta attgaagttc
     2401 aagatttatg ttcatatgta gatatattaa attataaggg agatttgata ttaaaaaaat
     2461 ataaggagat acctataaag aaaaaaatgg ttaaagtgaa aagagatata cgataattta
     2521 gttattactg ctctctctga tgatgaaatc atatatttaa gtaagagtat caatatttat
     2581 gaattattct gttatcgaaa tgaagtttca aaaaacaata gaagttgtta aggaaaggaa
     2641 tgttgacttt atctacattc taagctatac tatttttaaa tgtttatgac aatagtcatt
     2701 ttaaatataa gtaataaata tatacctata gggatattaa aggaggtaat atatggcata
     2761 ttttaactat aacgacaaac agtgctacta taatgaaatt ggggatggta caccattact
     2821 ttttttacat ggaaatacag cctcttccaa aatgtttaat gatataatcg atttttataa
     2881 agatgaatat aaagtaatat taattgattt tgtaggtcat ggaaaatctc aaatggtcga
     2941 taaattttct gctgacttat ggtttgatga ggctatgcaa gtgatatgtt ttttagaagc
     3001 aatgaactat aaaaaagtaa acataattgg tagtagtgga ggagctcttg ttgcattaaa
     3061 tgttgcactt gagagacctg atttagtgaa taaggtaatt gcagatagtt ttgaaggaga
     3121 agttccttta gagtcatttg tacaaaatgt taaaatagag cgtgaggcat caaagcaaga
     3181 tgatggtgca aaagcttttt atatttataa tcaaggagaa aactgggaaa aagttgtaga
     3241 taatgataca gaagctattt ttgaacatta taaaactata ggaaaatttt ttcataagcc
     3301 tctagaaact atgcaaccag aggtgctttt aacaggaagt agagaagatg agtttgtatc
     3361 tctaatatct agtgattttt ttgaaaatac tttttcatta cttcttgaaa agattaaaaa
     3421 tggtaaaatg tatttgtttg ataaaggtgg tcatcctgca attttatcga atggattaga
     3481 tttttcaaat gtagcaaaga aatttttaga ggaataattt aaccaaaatt tacaataatg
     3541 aaaactgtaa aatggattgc aaaacttaca aaagtgagtg tacatatact tcattattat
     3601 gatgaaatag gattgttaaa acaaaatatt ctaatagtca agttattaca tttgtatatg
     3661 caatatgtat agaaagagat atcattagat tgtaaagttg ggattaaata acaatatgaa
     3721 attttattta ttcagatatt gtagcttaac tatataaatg ttagaataaa tttatatagt
     3781 taagctgtag gattaaataa tttagttaaa taagtctatt atctcttcat cagatagact
     3841 ttttaataca ccttcattag aaagttctcc atttataaat tgatttataa gctctttttt
     3901 actttcttgt aattttatta ttttttcttc tatagtccct ttagcaatca attttataac
     3961 ttgtactgaa tttttttgac caaatctatg agctctatca ctagcttgat tttctactga
     4021 tggattccac catgggtcaa agtgaattac catgtcagca gatgtaagat ttaaaccagt
     4081 tccgcctgct tttaaagata ttaaaaaaac ttttttatcc atactattat tgaactcatc
     4141 tacaagttca agtctttctt tggcattagt tttaccatct atataatggt atttaatttt
     4201 atacttatca agttccttac ttatgttctt aagcacagaa gtaaattgtg aaaacaataa
     4261 tattttgtgg ttttcgttta tactatccct aagtatttct aaacatgttt caatttttga
     4321 acttttttta ttgtaatctt ttactactat acttgggtct aaacaaagtt gtcttagttt
     4381 agttaaataa gaaaatataa ctattttatc tttctttaga tttttatctt tcattttatc
     4441 ttgtatatct tttgaatata cactatatat ctttttctgc tctttattca attcaacaaa
     4501 aaagtttttt tcaatcttat ctggaagttc tttcataact tgttttttac ttcttctaag
     4561 aataaaaggt tttattaatt ttttcaggtt tttaacatta tcctctttat ttataaacaa
     4621 ttcctgaaac tttgctttac tatatagata gccaggcatt ataaaatcaa atatagacca
     4681 taattctagt agattatttt ccataggtgt accagtgagt gcaaatttat tttctgcatt
     4741 tatacttttt acagactcag ttactaaagc tactggattt tttatatttt gagcttcatc
     4801 taatatacaa taatcaaact ttatttcact atatttttct aaatcatttc taagagtacc
     4861 atatgttgta agtattacat caaaattttc taattccata aaacattttt ctctatctct
     4921 tttattccca tgtatcaata aaacttttat atctggagca aacttttcaa attcattttt
     4981 ccaattataa atgagtgatg tcggtgtaac aataagactt tttttattag ataatgataa
     5041 taaaaatgca atagtctgta ttgttttacc aagacccatt tcatcagcta atataccacc
     5101 aaatttataa taatctaaaa ctttaaacca attgaggcca tttatttgat aatcacgaag
     5161 gtttgcattt aaatttgcgg gaatttctaa atttatgcta tcaaaatttc taaatttttc
     5221 acatatactg tctagttctt ttttaccatc tataaacttt aaatttttac tacgtattac
     5281 atcatttata tatagagctt tatttttata aattttatta ttttttgatt tatccattat
     5341 atctagattt tcaactaatt caaaaaaatc ttttgtttct ctttctgata aatctataaa
     5401 gtttccatct ttaagtttat aaaaagtacg tttttcttga aaagctttca aaatatcctt
     5461 gtactctttt ggatttatat cagatatatt aaaattaaat tctaggtaat tctggttagt
     5521 ctgattgatt gaagcttgaa ttgatgaagc gttatatatt tttttcaatt taaacttctc
     5581 agaataatat atatctccaa attcctttag agattctaat tgtaaatcta aaaatgtaaa
     5641 taggtcatag tcagttcctt taaacacata atgattattt tcctttgtaa attgtgaaga
     5701 agtaagttta ttttcaatag tagtttcctt ttgtatgttg gggagcacaa attttttatc
     5761 ctcatcatca tatatatact ttacatcaca acatatgttg ccatcaatca tatcaaagta
     5821 gaactcagat ttaaaatctt ttgttatatt attttttatg ttatcatcta aatgaacttc
     5881 ttgagatata gattttaatc taggaactag caatcctaaa acatccttag aatcctcttt
     5941 atcaaatttt aactctttag tctttgataa tatttgatat atttttgaat aatcatgagc
     6001 ttttttatgt gataataaat agatattgcc atcatataaa agtacatcat tttttttagt
     6061 aagtggaaca ggtagtgaat ctttagaata taagactatt ttatcttcta tatttttaaa
     6121 atcaaacctt acaggtagag tatcatttaa tatctttgga tgatattcct ctttagaaaa
     6181 aataagggta aaatccttgt acttaatagc atttaaaaat tgttttaaag agcttgcatt
     6241 tatctccata aatttttttg aatgtgatga agaaaagttg tatgaaagat ttgtcccata
     6301 ttcttctata atatctacta acttctcatc ttcatcagca aaataatgaa tgttggggtt
     6361 gtaagtaaat ttcttcccaa aatctaattc atatccatta agttttgaat atgcaaattc
     6421 agtaatgttt tttaaaatgt acattttatc aattcctatt ttaaattcag caattgcttt
     6481 atttgaatcc acatttatca atacatctaa attaaccctt tctcttttag tattgtctaa
     6541 caaagaaagt aattgcttgc ttaaatctaa gcttgtttta gaatttgtat tagaaagctt
     6601 atccttatct gcacctaata tatctattag aataggagga acatctcttt tattttttat
     6661 ttgttttaag ctttttatca caatagcaac tatatgctta catataaaaa tacttttgcc
     6721 ctttgattga cagtcagcgc aactacaatc attttcaatt atcttaaagt gctttatatc
     6781 tatagttatt gaattaaaat aaaagctata attatcttca gaattgactc caccacgaaa
     6841 tgtcaacagg ttattttgta ggtcatcaat atctatgtct atataatcaa ctaattgatt
     6901 agtataatat tttacacctc ttgaccatct tgatggttct gtatttcttc ttattagttt
     6961 atatatcata tctattttca tattattcac cttacctcac tgtatatatt ataatctaaa
     7021 tgcattcaat ttacactcta aatttaagac taaaattaag gcagatatga atagctttta
     7081 tctattacaa tatatgtgta aataacaatt tactattaga tttttattga attatcttat
     7141 aaactatgaa tgaaaaataa ttatataaat agattattaa taaatataaa atcttattat
     7201 taaaaatatt cttagaaatt gcactatata aaatataaag tatgatattt gaataaaaag
     7261 aatgtataat aagtataata atttgtattt attattggga aaggaaaatg ttaatatgga
     7321 tagttataga caacttaaag ctaaaatacc ctgtaaggaa acaggtatag aaataaagag
     7381 gactttatgt gatatttgtt ctccaggagt acattgtgga attgatgcat atgttaaaga
     7441 tggtaaagta ataaaaatag aaggtacaaa agaacatcca atgaataaag ggttgttatg
     7501 taccaaaggt ctttgcaata gagaatatat atatagagaa gatagaataa aaacacctct
     7561 aaaaagaatt ggaaaaagag gtgaaggaaa atttaaacca atatcatggg atgaagctta
     7621 tagagaaata gcagaaaaat taaatagagt aaaatctgag tatggagcaa agcatgtagc
     7681 tttttttacg ggttatggaa agtggtatag accttttttg caaaggtttg catcttcatt
     7741 tggttcacct aattatggta cagaatctag ttcatgttca acatcagcac agatggctgg
     7801 aaatgttgct actggaagaa actttacaat gcaagatatg gagaatgcaa aaacttattt
     7861 agggtgggga tttaatccat actactctaa atttttaaat agtgtgaatc taataaaatt
     7921 aaaagaaaaa ggtctaaaat ttataattgt agaccctaga ataacaccga caactcaaaa
     7981 actagcagat ttacatttaa gaattaaacc tggaactgat ggagctttag ctcttggaat
     8041 ggcgaatcta tttatacaaa aggatttaat agatagagaa tacatagaaa agcatgtaca
     8101 tggatttaat gaatataaag aatatgtaaa attatttgat ttagatactg taagtagaat
     8161 tacaggagtt tctaaagata atatagtaaa agctgttgag atgttgtcac aaggtccatt
     8221 ttgtataaat gaaagtgcag ctccaatagt tcatcatcaa aatggatttc aaaactatag
     8281 agcaatgatg tctttatctg ctataacagg taattatgat agaataggtg gaaacatacc
     8341 aaaacaagat tactttgctg gtaagatagg tggattccaa acactagaaa aagagtttgt
     8401 aaaagaagtt ctacctaaaa atataaatga aagaattggt ggagaaagat ttcctctatg
     8461 gaatgaaatg gtaactcaat ttcaagctat ggatttacct aggcaaatag aagaaaagac
     8521 accatatgaa ataagagctt tgttttgtca tgggataaat gctagaatgt ttcctaattc
     8581 agggagactt tttaaagcgt tgcatagtgt agattttttt gtgaatatag atttatttat
     8641 gacagataca tcaaaatatg cagatatact tttacctgcg tgtacatcat ttgagagaga
     8701 agaatgtaag agttacccag gagggtatat tacatatacc aaaaaagtta ttgataaatt
     8761 atacgattca aaatcagatg tagaaatact ttcagattta gcaaatgtta tgaatataga
     8821 tgatgattta ttaaaagcag gttatgaagc aagtataaga catatgttta aaaagacgtg
     8881 tgtagatgta gataaactta aagaaagtga tttaccatta aaatgtaaag attttaagcc
     8941 atatatagta ggctcatata caagagaggg gtatgatact agcacttcta ggtttgaatt
     9001 aaaatctaca attattgaaa aatataaaga ttttggactg gatgcactac caacatatag
     9061 aggatttaat gataatatag ataatgaata tactcattta ttgacatcag gagcaagaat
     9121 ttcaaatgca ctacattcaa gacttcatga catcaaactt tcaagaatat taaaaaatga
     9181 agctagttgt gatatttcca gcttggatgc aaaagaattg ggtgttatca gtggagatta
     9241 tataaaaata actactaaga taggaagttt ggatgtaaaa gtcaatgtta ctgatagagt
     9301 cttatataag aatatacata tgtatcatgg atatagagaa tctgatataa atagtattat
     9361 tgatgataat tttgaccctt atagtggatt ccctgcgtat agaagttgca agtgtaagat
     9421 tgaaaaaatg aatagataaa tggagagata taatgaaaag tttatttaaa tttaataaag
     9481 aattatgtac agcatgtgga gcatgtttgc ttgcatgtat agaccaaaat gataataata
     9541 tagacaatgg tgaacttttt agacatgtat ttacaataga ggaaggtgaa aatatagaat
     9601 atttttcatt agcatgtatc cattgtaatg agccaaaatg tttaggtgca tgtcctaaaa
     9661 attgttttaa aaaagaagaa ggattcgttg tattagacaa tcaaaactgt atagggtgta
     9721 aactttgtga aaaagcttgt gaatatggag ccttaattta tggaaatgat ggtaaggcaa
     9781 ataagtgtaa tggttgtatt gagagatata agtgtggtat ggattcacct tgtgtaatgg
     9841 tatgtcctac aggagcatta aaatttaata aataaattaa atatagagag tgacttatac
     9901 ttggtataag atatttttac aaaaagctat attgagtttg ttataataaa aactcaatat
     9961 agctttttta tggatatagt tggatactat aatgtgattt ttagtaatag ttttttgcta
    10021 ttggacaaag actgtaaaac atattaaact tatgttgttc aaagtcgaag tataatataa
    10081 taactagaaa attgatttat agtgttataa aagcaaattt agtaaggtat aaaataagag
    10141 gcaatctttt actacagtat caatatttgg gagttatgtt atggcagagc cattaaacaa
    10201 ttataatggt acaagtgatt caacctttga aaatgtatta tataaaaaat taaaataaga
    10261 ttaaaaagga gagaataaat tatgaaaaaa cttaaattaa ttattatgct tgccttggta
    10321 ttagcacttt taattacagg atgttcatca ggaaataata aaaataaaga agaacaaaaa
    10381 actataagag ttgcaacatc aggaacatat tacccttttg cttttaaaga taaagataaa
    10441 cttaaaggat ttgaagtaga tttttgggat gaatttgcta aacgaactaa ttgtaaagtt
    10501 gaatgggtat taactgattt tagtggatta tttggattat tagaagctga taaagcagat
    10561 gttgtatctg ctcaacttac tgcaacacct gaaagagaga aaaaatatgc tttttcagat
    10621 gcttataatt atagtggaac aaacattatt gttagagaag atgatactag tataaaatca
    10681 atagatgatt tagttggcaa gaaagtaggt gtgggtactg gagcagtagc aaatgaaata
    10741 ctaaaagaaa agtacccaaa tgatgaaata aaaatagtaa attattcaag tgcaactctt
    10801 aaagggaatt atcaagatct agaaatggga agattagatg cagtagttgc gcaggatgtt
    10861 gaggctttaa tagctataaa agaacaaaaa ttaaatctta aaatggtaga acctccaatt
    10921 caattcggag cctgttcttt tgcgattcaa aaaaatgaaa aaggtgaaaa atggacaaaa
    10981 gaaattaacg aagtaattga agagatgcat aaagatggga ctttgactaa aatatctgaa
    11041 gcctggctag gaaaagacat tactaaggag cctattaatg agtaatttta attttgaatt
    11101 ttgtctaagt atatttccat ttttgattaa agcattggga ataacaatac taatatctat
    11161 tgttgcattt tttttatcaa tattattagg tgtaatactt gcctttttca cacagacaaa
    11221 atcaaaagtt ttgcaataca taacaagagt gtatgtttca ttctttagag gaacaccact
    11281 tttaattcaa atatttttct tttactttgg atttccaatg atattttctt ttatgaaaga
    11341 ttgttcacct tatttagcaa tagtaatttg tttaggtgta aattcatctg catatatgac
    11401 tgaaattata cgagcatcta ttgagagtgt tgataaagga caacaggaag catgttatgc
    11461 ttttgggtta agtaaatatc agtcaatggt taaaataatt ctacctcaag cagctacatc
    11521 tgcagtacct cctattgtaa gctgtacact ggacataatt aaaggttctt ctttaggtct
    11581 aactatagga atacaagatt taatgggagt agctcaagta gaggctgctt caagttttaa
    11641 atattttgag atatatatga taacgatatt aatttactgg gcagtatcta taatattgag
    11701 taatatacaa agcaaagttg aaaaaagaat aggaaaggca tattagattt caatatttaa
    11761 aaatgggatg atgtagatat attattactt gtaaataaat ttttaatttt agaaaaataa
    11821 acttagtaat ctaaattatt gagaaaatat agtatcaaaa tggactgtat aatcaagtta
    11881 aaaaatagtt tttctattga aaaaataaat aaaattctat tgtaatttaa tattcgacaa
    11941 agttttataa aaaacaacat taagctaggg ggacttattt tatgattttt aatatgaaat
    12001 attttttaga attatttcca ataataatga aatcgttcaa tgtaactgta attttggcaa
    12061 taagttcatt agtattttca ttaattatag gtacaatagt agcattatca tcttattaca
    12121 aagtgaaagt attgaatcaa atatgtaaag tatatgtatc tatctttaga ggtacgcctc
    12181 ttatgccaca gttattcttt ttatattttg gactagcgta tatgaacgat tttgttaaaa
    12241 atatggaccc agtatttgca acatctatag tacttagttt aaatatggga gcttatatgt
    12301 ccgaaacgat aagggcatct atactttcaa ttgataaagg tcagatagaa gcagcatatg
    12361 taatgggatt aacaaattta caaacaatga aaaggatagt gataccacag gccgtaagag
    12421 ttgcactgcc atcattattc aataatttta tagatttaat taaagggtct tctgtagctt
    12481 ttgttgttgg agtaaatgat ataatgggtg ctgccaaatc acaaggagcg ttgagtttta
    12541 agttctttga agtgtatgga gcagttatgg taatttactg gtgcataata actcctttag
    12601 ggtgggggca aaaaaaatta gaaaagaaaa taagcaatta ttaagttatt aagaaaatac
    12661 ataaaagtat ttttatataa aaaatggtgt gaggagaaga gatatggtaa aaagaaatat
    12721 tataaaatgt ttagtggcag ttttatcgtt aacatttgta gttgctggtt gttcaaaaaa
    12781 agaggaagta aaggatgaaa aagttataac tgtagcttca tctaattcag ctgacccata
    12841 ttcttatgtt gataatggtg agcataaggg ttttgaagtt gatatgtgga aagagattgg
    12901 taaaagaagt ggatataaga taaaaatgga accaacaggt tttagttcta tatttgggat
    12961 gttagattct ggtaaagttg atgtggcagc caatttcttt ggaatgtcta aagaaagatt
    13021 agaaaaatac gatgcttcta taccttatgg ttctgattct gtatgtatag ctgtaaaaga
    13081 tggaaacaac gaaattaata aacttgaaga tttaaaagga aaagttgtag cagtttcaga
    13141 aggttctcaa gggcaacagg ttgcagctag tgttaataaa gataatttgt ttgaagaaaa
    13201 ggtttatggt gatggaacta atggtcttca agatttagat ttaggaagag ttagcgcatg
    13261 gattgatgca gaacttactg ttgtaggaga cgctaaaaaa gctaatatga aggtaagagt
    13321 attagatgaa aaattatcag ttactaatat agcatacttc tttaagaaaa atgatgaaaa
    13381 atctaaaact ataaaagaag atgtaaataa agcaatagaa gaaatgcttt cagatggaac
    13441 agtgaaaaaa atatctgaaa aatggtttgg aatggatgta agtgctgata tacaaaaata
    13501 aatactttaa aattaaaata taaaactgtg ataactatag ataagtatac taaatttatc
    13561 tatagttatc tgtgtattac aatgtattta gattagtctt tataaattaa aatgagttat
    13621 ggaggtcttg caaatgattg aaactaaaat aaaatccatt gcactaaaaa atagagaaaa
    13681 aatcataaaa ataagacgac agatacacag taatccagag ttagcattta aagaatttaa
    13741 aacatccaaa cttataaaag aagagttaaa taaacttaaa atagaataca tagatgttgc
    13801 tggtacagga gtattagcta ctataaaagg aaaaaataat ggtgggaaaa ctatattgct
    13861 tagagcggat atggatgccc ttccaatcaa agaggaaaat gatttagaat ttaaatcaat
    13921 aaatgataat atgcatgctt gtgggcatga tgcccatgtt tcatggttgt taggaacagc
    13981 aatgatttta aatgacataa aagaagaatt gaatggaaat gtaaaattac tatttcaacc
    14041 tggcgaagaa aaaggtggtt ctgatattgt aattaaagaa aatgtacttg aaggtgtgga
    14101 tgcattagca actggtcatt gttggccaac tattgaatca ggaaaaattg gaatcgctag
    14161 aaattgtgct atggcagcga ctaatacttt tgaaataaca ataattggca agggcgggca
    14221 tggagcagag cctcataatt gtatagaccc aattgcagtt ggaaatgcag tatattcatc
    14281 cattcaacag atagtttcaa gaaaaattga cccagttgta cctgtagtag tgtctgtttg
    14341 ttcttttaat tctggtgttt ctaaaaatat aataccagat gtatgcacac ttcaagggac
    14401 tattagagcg ataagcaagg aacaagtaat tgaagtaagt aaaattctag aaaatatagt
    14461 aagaaatgta tgtagagcat acggagcaga ttgcaaattt gaaaagagta caggaggaga
    14521 tgctgttata aatgacaaaa acatgataga gctaggaaga gaaagtgctt gcaaaatact
    14581 tggagttgaa aatgttgaaa taattgattt tcctgcaatg acaggtgagg attttgctat
    14641 ttatatgaaa gaaaaacctg gtttatttat gtatataggt gtaggaaata aagaaaaaaa
    14701 tataaactac agattgcata gtaacaagtt taatatagat gaaaaatgtc ttagtatagc
    14761 atcttcatta ttctcacaac ttgcagtaga ttatttatgt caactataga gtacattcat
    14821 aaaaatatat ttagcctatt tttaaattta atgctagtat attaattttg atgtagaaca
    14881 tattatttat tatatttatt taaaaaggtg gataacaatg ataaaagtag aaaatttaca
    14941 taaaagtttt aaagggacag aagttcttaa aggtataaat ttagagataa atcaaagtga
    15001 agtagtagct atacttggac catctggaac aggtaaatct acgttactta gatgtttaaa
    15061 ttatttagtt gagcctacaa aaggaaagat aatgattggt gatgtaaaag tagatgtaga
    15121 aaaagcaaca aaaaaagata tagccaattt aagaaaacat acatctatgg tatttcaaaa
    15181 ttataactta tttaaaaata aaacagcatt agaaaatgta atggaggcat tgatagtagt
    15241 acataaaaag agtaataaag aagctagaga aatatcttta aaattaatag ataaagtagg
    15301 tatgttggat aggaaagatt tttatccatc aaaattgtct ggggggcagc aacaaaggat
    15361 aggtatagca agagcgatgg cagtaaatcc aaatgtaata ttatttgatg agccaacatc
    15421 tgcacttgac ccagaacttg ttggagaagt tttaagcgtt ataaaatcgc ttgcgaaaga
    15481 aggaataaca atgcttattg taactcacga aataaatttt gcaaaagaag ttgctgataa
    15541 aattatattt atggataatg gtgtaatagc tgaagaagga actcctgatg aaatatttaa
    15601 taatcctaaa aatgaaagaa ctgcaaaatt tttaaatatc gttaggaaag aagaagtatt
    15661 tagttaaaag tgtataaatt aagtattaga agatatgtaa gatactccca ttaaatttat
    15721 aagttagata ataaatttaa tggaagtatc tttttattat tagatttatt atttaattta
    15781 aaattagaaa tgtaactatt tgaaaattct ttttaaaagt atatcaaaaa tatcaagtac
    15841 atgaaaaaat aatatttgac aaaatatcat atatgtttac aaaattaact tgatggaatg
    15901 ataaagcata ggaattacaa tgagtatact aaaactttat attaaagtac aaattat
//
LOCUS       DAELXX010000092        15894 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.092, whole genome shotgun sequence.
ACCESSION   DAELXX010000092 DAELXX010000000
VERSION     DAELXX010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15894
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.092"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            495..1145
                     /locus_tag="KPH29_002773"
     CDS             495..1145
                     /locus_tag="KPH29_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG1468356.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEEV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
     gene            1423..3489
                     /locus_tag="KPH29_002774"
     CDS             1423..3489
                     /locus_tag="KPH29_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBG1468357.1"
                     /translation="MKEVVESLKKITKERIENTMKKLFTSETFLEYQFISDCQISPDG
                     GYTAFIVKKADIKENGYTSQVYILNNKTGELKQITSINSVGAYVWEDENTILFPALRN
                     EKVKEAVKNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVIDSYDNDRP
                     IVEGLPEEEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYIYDSNINKLKAI
                     TAPMFNVVGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNICILEKDKYIIKGF
                     ELYQNQVILNLTDALSYGNGENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGA
                     GQTTKVDGEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCL
                     GNGLPEVYTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYE
                     VGKKYPAVLAIHGGPRLTYGPYFMHEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQ
                     FGDIDYIDFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRS
                     IANWSSMEGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYADKCVTPTLFLHGEKDY
                     RCYMQEAFQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIK
                     KDNIGQ"
     gene            3506..5461
                     /locus_tag="KPH29_002775"
     CDS             3506..5461
                     /locus_tag="KPH29_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBG1468358.1"
                     /translation="MEKIKIESSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKAGTTDFYTISIEGGE
                     AKEAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDR
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGIDVSSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLERSITQNHDIFILDLST
                     SEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSKPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAVSQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            5477..6667
                     /locus_tag="KPH29_002776"
     CDS             5477..6667
                     /locus_tag="KPH29_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBG1468359.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVIVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
     gene            complement(7515..7698)
                     /locus_tag="KPH29_002777"
                     /pseudo
     CDS             complement(7515..7698)
                     /locus_tag="KPH29_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427897.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7801..8175
                     /locus_tag="KPH29_002778"
     CDS             7801..8175
                     /locus_tag="KPH29_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG1468360.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFVSTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMGILYEED"
     gene            8177..9034
                     /locus_tag="KPH29_002779"
     CDS             8177..9034
                     /locus_tag="KPH29_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1468361.1"
                     /translation="MENILEVYNLNKTYTNFSLSNISFVLPEDCITGFIGVNGAGKTT
                     TIRSILDVVKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMSEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTEYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDEIKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            9037..9675
                     /locus_tag="KPH29_002780"
     CDS             9037..9675
                     /locus_tag="KPH29_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBG1468362.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFILWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVMIIMASSFV
                     LPLTIAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
     gene            10649..11383
                     /locus_tag="KPH29_002781"
     CDS             10649..11383
                     /locus_tag="KPH29_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG1468363.1"
                     /translation="MNLAKGLDVLKISSNVFGEDKVMYIPVIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDRLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEVSYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMVLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELTPVDVMHSNKQELKDAIKNISNYD
                     IETIVFSHGGLYQRDIIGTLKNLIIE"
     gene            12525..13781
                     /locus_tag="KPH29_002782"
     CDS             12525..13781
                     /locus_tag="KPH29_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBG1468364.1"
                     /translation="MIEKFKTLIWIKMIIRIKSVYKTYGGLFLLGLIGIPVGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIISGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNASSIFLV
                     TGIAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASAVSKTLGLEKFS
                     FALSSKVVFDLPIFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
     gene            13844..15037
                     /locus_tag="KPH29_002783"
     CDS             13844..15037
                     /locus_tag="KPH29_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBG1468365.1"
                     /translation="MLTSLALIFLLGMASGGIFEKIKLPSLLGMLLTGIILGSYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIIGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIVGAVIGICLAVFFKKVHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMNIGIFLQIKYRVVARRLSVKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGIASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     EGKSK"
     gene            15244..15624
                     /locus_tag="KPH29_002784"
     CDS             15244..15624
                     /locus_tag="KPH29_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG1468366.1"
                     /translation="MYLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKILSKLSKKRFLYVQETGKCMYYTVAIKEKTYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYI"
BASE COUNT         5873 a         1760 c         2787 g         5474 t
ORIGIN      
        1 tactttttgt aacatattat tcaaattatt aagagatagt aaatatttat agtttaattt
       61 gtagtacact agcatgattt gcagaacaat gaattaatct atagtataat agtttaattt
      121 gaagtacaac gatttaatct atagtacact agcatgattt gaagtataac gatttaatct
      181 atagtacaat aacttgattt gcaatacaat aatttaatct atagtataat agcatgattt
      241 gaagtataac gatttaatct atagtacaat aacttgattt gaagtacaac gatttaatct
      301 atagtacaat aacttgattt gcaatacaat gatttaatct atagtataat agcatgattt
      361 acagtacaat aatttaaatt gtagtacaaa agtactatca aataagaatt tctactttaa
      421 aaatattact aaaagtaatt aaaatccaat aattcaatat aatatggcaa aagaatagaa
      481 ataaggtgag agtagtgaaa gtaaagtcaa atttagatga aattttatat cataagatta
      541 ttgaaagtct tattcgtggt gaatactctg taggtcaaaa aatattacta aatgaccttt
      601 gtgaaaaatt tgaagtaagc agaacaccag ttgtacaagc agtaaaaatg ctaaataaag
      661 atggagtgct tactattatg acaaatggta aagtgtacgt accagagtat gaatatgata
      721 tggtaaaaca agtttgtgaa acgagaacac tgattgaaac atatgcttta gaaaaaatga
      781 tgcaagaaga ggaagaggtt tttcaacaaa agctagatgt aataaaaaga tactctgata
      841 aatgcgagga gtactatcgt caagaaaaat ctgtagaact tgcactagca gatttggaat
      901 tacataaagc cattgtagaa ggggcaaata atgagatttt aaatgatgta tatgttggta
      961 tacaaggaag atttatagta gtgaactatt taattcgacc attaaaaaat cgtaattatg
     1021 aaggaacagt acaagaccat tttgaaatat taaaattcat tgaagacaga gatacaaaaa
     1081 aagcagtaga taaactaaga aatcatattc aaggaactat taaaagattc tgtgaagatg
     1141 aataataagt tttgaagtta ctatatactt atctattaag ttgctaaatg tagtctaagt
     1201 ataatctaat tatagtttaa acataatcta aatataatct aaatatagtg taaataagtc
     1261 taagtatgaa ttaaatatag attaagtatg aattaagcat ggattaaata tactttaaat
     1321 tacaaatatt acaaggaagc cagaaggctt cctaaaaaaa taacctttat aaaatttttt
     1381 attttaaatt taaaattttt tattttaaat ttaaaataaa aaatgaaaga ggtggtagag
     1441 agtttgaaaa aaattactaa agaaaggata gaaaatacta tgaaaaagct atttacgagc
     1501 gaaacatttt tggaatatca atttataagt gattgccaaa tatcaccaga tggaggatat
     1561 acagcattta ttgtaaaaaa agctgatatc aaagagaatg gttacacaag ccaagtttat
     1621 atattaaaca ataaaactgg cgaattaaaa caaataacgt ctataaactc tgttggagca
     1681 tatgtctggg aagatgaaaa tactatcttg ttccctgctt tgcgaaatga aaaagtaaaa
     1741 gaagctgtca aaaacggtaa gcaatgcatg agttactatg ctttatcttt aaatggtgga
     1801 gaagcagaag aactttttag actccctatc aaaggtggta aattgaatcc tataggaaaa
     1861 ggtttgtatg cagttattga ttcttatgac aatgaccgac ctattgtaga gggattgcca
     1921 gaagaagaac aacagaaaaa aataaatgct tataacaaaa gacattatga acatttcaaa
     1981 gaaattcctt atgctgtcaa tggggaggga tatattagca gaaaaagaaa acgcctatat
     2041 atatacgaca gtaatataaa taagttgaag gcaattacag cacctatgtt taatgttgta
     2101 ggtatgaaaa taagtgatgg aaaaatctta tatatagggc aagagtttaa agatgtaaaa
     2161 ggtttgaaaa atggggtcta tgtatttgat acaaacacaa acacaaatat ctgtatatta
     2221 gaaaaagata agtatattat aaaaggtttt gaattatatc aaaatcaagt gatactaaac
     2281 ctaactgatg ctttatctta tggaaatgga gaaaatggag atttttatac tattgatatt
     2341 gatacgaaag aaatgaaact tctttcaggg catcaacatc attgtattgg aaatacagta
     2401 acatctgatg ttaaaatggg tgctggtcag acaacaaagg tagatggaga atatatttat
     2461 tacacttcta cagtagatat ggactgcatt attgaaagaa tacatattcc aacaggaaaa
     2521 caggaaaaag taacacagac aggttcagtt gattttatag atgtaaaaga tggcaatatt
     2581 gtatgtgtgg gttgtttagg aaatggatta ccagaagttt atacagtaga gaatggaaca
     2641 ttgtgtaaga aaacacattt aaatgaccat attttagagg aatataaaat ttctgtgcca
     2701 gaatacatag agtcaaaagg aagttctaaa tgggaaatac aaggatacgt atataaacca
     2761 gtagattatg aggtggggaa aaaatatcca gctgtattgg cgattcatgg aggaccaaga
     2821 ttgacatatg gaccatattt tatgcatgag atacaggtat ttacaagtgc aggttacttt
     2881 gtattcttct gtaacccaag ggggtctgaa ggtcgtggca atgcttttgc agatattagg
     2941 aaacagtttg gagatattga ttatatagat tttatggaat ttacagatac tgtacttgaa
     3001 aaatatacag acattgataa aacaaaattg gctgttgaag gtggaagtta tggtggattt
     3061 atgacgaatt ggataattgg tcatacaaac cgttttgctg ttgcttgtgc tcagcgttct
     3121 attgcaaact ggagtagcat ggagggtaca acagacattg gttattattt ctgcaaagga
     3181 caaacaggtg cttctcatat ggaaaaccac gagcttcaat ggaaacagtc acctcttgct
     3241 tatgctgata aatgtgttac accgacttta tttttacatg gtgaaaaaga ctatagatgc
     3301 tatatgcaag aagcattcca gatgttttca gcattgaaaa ttcatgggtg tcctacaaaa
     3361 ctttgtctat ttgaagggga aaaccatgaa ctgtctcgtt ctggtagacc aaaacaaaag
     3421 ctacaaagat tagttgaaat gctagattgg ttttctgtgt acataaaaaa agataacata
     3481 ggacagtaga aaggagaata taaaaatgga aaaaataaag attgaaagtt cagatatttt
     3541 tgaatatata ttccctcatg atatttcttg ttcacctgat ggaaagcata ttgcttatat
     3601 tatatcaaat atcaacgagg aaaaagactg ctatgaacat gacttatatg tgatggacat
     3661 caaaacagaa aaacaaatac atatgactca aacaaaagat gtaacatcat ttagttggat
     3721 ttctaataca gaactacttt ttacatctaa aagaaataag ccaaaagcag gtacaacaga
     3781 tttttacact atttctatag aaggaggaga agcaaaagaa gcattttcta ttccaaaagc
     3841 ttgtagcgta cctgtatcac ttggaaataa attgtggtta ttgacaacaa aaaatccaac
     3901 ggattctaaa aaatcagaac cagatagggc agtagagggt gtagattatt ggacatttac
     3961 agataaacct tttataagag atagagaaaa tttttctcaa agaagacgtg taacattaga
     4021 attatatcaa gaaggtgaaa atataacaaa agccattaca ccaaaatttt gtgaagtagc
     4081 aggtatagac gtttcttctg ataaaaacag aattttatat actggtcaga tttatgaaga
     4141 ttgtgcaact cctttttctg gactttggga gtatcatata gacagtggtg aaaccaaaga
     4201 acttgtacca caaggaaagt accaaataag tttggcaaaa tatattggca aagataaagt
     4261 aatgttgcaa gcttcaacat tagaacgttc tatcacacag aatcatgata tttttatact
     4321 tgatttgagt actagtgaaa taaatatgat tgctagtcca gatggtatgt atgctacatt
     4381 actggatgta gatgcagtat atggtggtgg acgtagtaat aaagttatag gagataaatt
     4441 tattggtgca agaatttgca gaactatgac agaatttaat gagtttgata caaagacagg
     4501 taatatacgt atcatcacaa aagtagatgc ttttacatct tttgatattt acgacaacac
     4561 catgtacaca gtaatgttga aagattatga gttagcagaa atatatagca ttgatatgac
     4621 tacaggtaca atgaaaaaaa tgactgcttt ttctaaacca tatttagata ctcataaggt
     4681 ttcattacct gaaaaattga cgtttgtagc aaaaaataaa gaagaagttg atggttttgt
     4741 tataccacct atagatgcta aagaaggcga aaaatatcct gctgtattgt tcattcatgg
     4801 tggacctaaa tgggcatatg gctatatgtt tacacacttg aagcagtgtg taacatcaaa
     4861 gggaatgtat gtgatttatt gtaaccctca tggtggtgac ggttatggtg aaaaattctt
     4921 agaaatggta gaaagatggg ggtatgttga ctatgagcat cttatggaat ttgtagatac
     4981 ttgtattgaa aaatatcctg ggattgatgc agaccgctta ggggttgcag ggggaagtta
     5041 cggtggttat atgacaaact ggataattgg ccatacagac cgtttcaaag cagcagtttc
     5101 tcaaagaggt attagtaact tgattacagc ttctttaatt attgactttg gggatagaat
     5161 tatgaagcag acttgtggag ataagacgcc ttggaatcat gaggaggttc tttggaatca
     5221 ttcacctatt aaatatgtga aaaatgttaa gacaccaaca ttgttcttac atagtgatag
     5281 ggattataga tgttttatgg gggatacttt ccaaatgttt acagcattaa aacagcttgg
     5341 tgtagataca gaaatgtatt tgttccatgg agatacacat ggattgtcta gaaatggaag
     5401 accatctaat agaattgcac gagcaaatgc tattgtagat tggtttgaaa gatatttata
     5461 ataagggggt tattctatga gtgatatgtt attatggttt agtgtcgtat tagttttatt
     5521 tgctattggg gatttaattg caagcaaaac aaaagcaaaa gtatcagctg tatttgttac
     5581 actactttta tttttgattc tatttgtcac aaaagcaata ccagctgata ttattgaaaa
     5641 agcaggtatg accgcagcag ctagttggag tgttccaatg attatgttta gtatgggaac
     5701 aatgttgaat gttaaacaat ttatagacga atggagaaca gtattgacag catggcttgg
     5761 aattgtagca gttatagtct gtgtctcatt atgtattcct ttgtttggaa aatcaacagt
     5821 attgacaagc ataccagtta taaatggagc tttacctgct actactatta tgacacaagc
     5881 agcactagaa aaaggactga cacttgcagc agctacagca acagttgtat ttgcaattca
     5941 gaagtttatt ggtacaccaa tagcttcaag agcagcgtta caagaggcaa accgtctttt
     6001 agtagaatat catgaagcaa aatcaaaagg cattgattta gcaaatgttg atactgataa
     6061 gaaagaagca gagaatacag gcacaaaagt aaaacaagct ttctgtgaaa aatacgataa
     6121 atattattct acaaatgttt gtatattttt tatagcacta ttctcttatc tagggtatga
     6181 actttcagag attattcatg taaattattc tattgtatgt cttgttgttg gtgttattgt
     6241 aacacgtatt ggtattgtac caaaagacat tttggaaaaa ggaaaaatca agggatttat
     6301 taacatggtt gtgtttgcag ctgttattcc ttctttagca aaagtttctt tgacagattt
     6361 aattagtctt tttgtaccaa ttgtgggtat gtttgcagct tctatcattg gtatattcct
     6421 tatgatgaag gtgttaccag gttggaaaat tatcggttct aaacctcttg cttttggagt
     6481 agggttttgt cagatgttgg gattcccaac tacatattta atttcaaatg aagtgtgtaa
     6541 tgctgttgga gaaacggaag aagaaagagc atatttgatg tctaagatta tgcctaagct
     6601 ggtagttggt ggtatggctt gtatgatttc tattgtagta gctggtctta tggtaccaat
     6661 gttgtagata ggtttcatag ttaaaaagtg gtgttaattt ataggagaag ctaaaaagta
     6721 gtattaattt ataaaataat tttatattag ttaatttgtt ataagaatat tttcttttta
     6781 tgattataat tttgatttta taagagagca gattttttat ttgctatgct aatagttata
     6841 atttgttttt atttgctaaa atgcaacttt aatttgattt atgtataata tagggtattt
     6901 aataatattt ttgaagaatt tttataaaaa attaaaaaga ggagaaaaaa gggagtgcca
     6961 caaagtgaac tttttagttc taaggcactc ttttttgtac gaaatcgaat atattttcaa
     7021 cgttttaaaa gtgagaaaga gacttatctc tagtttggga tagccaaatt taattattaa
     7081 aattattttt tgttttattt atcaaagagt attcgtgttt tagaagttag tattttttat
     7141 tttatatatc caaggttttc cgtttctaat gttttctttt ttaaaataat tagggtattc
     7201 aattatagta aaaggtggat ggtattttgg aatgatatta tttaaagcac agtattgaat
     7261 gttgaaatcc tcacatttta gagtattatc ttttgtaatc aaaaaaatct tctatactta
     7321 tagcaaggtt acttctgcaa atattccatg catcataata tccaaattta aattttttta
     7381 atatagagtt tacctctagt ctacactctt gaataactct ttcctctatc caaattctta
     7441 taaactcatc ccatttatta ctatctattt attactatct aaatttttta gtatgtttgt
     7501 aattggaaat ggatttattc ctttatgttt tttataaata gtttataact taatttatta
     7561 ttaaacaaat cattcataca gaattacttt ttcttttaga tattcaattc ttacttttaa
     7621 taaatattta atacattcta tcttatgtgg ttttaaaaag tgtttatggc tatcaataat
     7681 ctctaatatt tcattcatat ttattttgtc atagtaatat tttacatctt ttatattgac
     7741 aaaaaagtgc tctaagtgta tagtgtgtat ataataacat cacactggag gtgataaaaa
     7801 atgaaagtgc tcatttctaa cacatcagat aagccattat accaacaaat taaagagcaa
     7861 attaaagaag caatatttac tgaagaactg aaagaagggg atgcaatgcc ctcaataaga
     7921 aattttgcta atgatttaaa agttagtatt ttgactatta gacgagtgta tgaagaactt
     7981 gaaaatgaag gttttcttac acgccaagtt ggaataggaa catttgtttc aactggaaat
     8041 ttagaaatac taagagactc aaagaggcgt atagttgaac aaaaaatggc agatatgatt
     8101 aaggatgcaa aagcactagg aattacacag atagagttac aagaaatgat gggtattcta
     8161 tatgaggagg attaatatgg aaaatatttt agaagtttat aacttaaata agacttatac
     8221 caatttttca ttatctaata taagctttgt attaccagaa gattgtataa ctggatttat
     8281 aggtgttaat ggagcaggaa aaacaacaac aattcgttcg atattggatg tcgtaaagcg
     8341 tgatagtgga aaaataaaaa tctttggtaa agatttcgat ggaaatgaga aggaattaaa
     8401 aaatcgaatt ggagttgtat ttgatgattc atatttctat gaggagttgt caatgtcaga
     8461 aatgaaaagt attattgcac cttcatatga taattggtgt gaagaagatt atcaacaaaa
     8521 tatgaagcgt tttaatctta atccaaaaca aaagattgat acactttcaa aaggaatgaa
     8581 aatgaaattt gctttagcat tagcattatc gcataaagct gagtttttaa ttatggatga
     8641 gcctacaagt ggtttagacc cactaatccg tagtcagata cttgacatac taacagaata
     8701 tatgaagaaa tgtggtcgtg gtgttttctt ttctactcat ataacatctg atttagacaa
     8761 aatagcagat atgattgtta tgattgacaa tggacgtatt atattagagc gtgaaaaaga
     8821 tactctattg gatgaacata gaaaagtaaa aggggatatt caatttctaa atgatgaaat
     8881 caaaaaatta tttttaaatt tagaaattac aggttatggt tttataggga ttacctcaaa
     8941 aattaatgaa gtgcaaaaaa atatgccaga tattttaata gagcgtccta caattgaaga
     9001 tattatgctt gcatatattg aaaggagaga gtaattatgt tgattcattt agttaaaaaa
     9061 gatattctga ttgtaaaaaa atatgtattg ttgatattgt ttattgcaat aggaatccca
     9121 ttatttatat tatggcgttt acctccattt gcacaaatat tgggatttgt actatcaatt
     9181 atattttcag aatttatgct gtgccagtat ctattattaa aggagagtca atattcaaaa
     9241 gcatctgtat tactttgttc aactccttat cctagacgtg gcttagttca atctaagtat
     9301 gttttatttg caatggtatt tatctattgt acattagttt actggattga aaatttactt
     9361 attccacaaa taggaacttt tgacttaaca tatattttga ttgttttatt aataagttct
     9421 attatttatg gagtttatat gcctattcaa tacaaattag gatatgaaac aacaaaattc
     9481 tttttcgtga tgataatcat ggcttcatca tttgtattac cattaactat agctcacagc
     9541 aattttgaaa ttccatttat atatcagtat ccatccatga tagtgaatat tgtattgttc
     9601 cttttaagtt taattatact gttagcttca atgtttattt ctattaaaat ttttagtaat
     9661 aaggagcttg tataaggtaa gggggaattt tatatgccac atttgccaga ttgggctgtt
     9721 ttactttgta tcttacttcc aatagtatat aaagcttgtc agatatttaa aagtaaaaaa
     9781 tagagtacat atttatattg ccatttttat tgagtgaagt tagatggtat gaatttaaaa
     9841 ggttacttga aaataaagtt acttggtgtg ggagagtaac atcataatat tatagattat
     9901 atgtttcttg tgaatcaatt tgtatttttt aatagataat atccagtaat tttattttcc
     9961 attgattatt ttcaattatg agtatgattg tgtgtattgt ataccatctt ttaaggtgtt
    10021 tttaaaaaat tatatcgtca aaatataaat ctaaggaatc ctataaataa agcttttcaa
    10081 ttttagaaaa ctaataaaaa gtaggaagtt aataaaaaga agctgtctca aaatagagat
    10141 aagcttcttt ttcattgctg aaatgacgaa aatataattg atttaatata aaaaagagtg
    10201 ctttatgaac tatttatttt gaacagtaga tacacttgca ctcaatttag ttactcctaa
    10261 gctttatgaa ctaaaagttt attttgaagc ctatttttct aaataatcat ctatatacag
    10321 tattgcgttt cttttattta ttcataattt attttcttat taaagttttt ctatattact
    10381 ttacttttaa ttttatacga attatcaaaa ataaaatagt tattagaaaa aaataaaagt
    10441 aattgcaaag attgcaatta cttacttcta tattaattac tataccataa aataacaaaa
    10501 aaatcaagtc gagtaatcct tcaacttaag tgatatacta ggtattaata atttttaatt
    10561 aataaaatat aactagagga aaatctacat tttctcaatt tctttaatta aattgacctc
    10621 taattaagta attataagga gaaaaattat gaatttagca aagggattag atgttttaaa
    10681 aatttcatca aatgtattcg gtgaagataa agttatgtat attcctgtaa tctacacaga
    10741 agatgatgca acacttattg acacaggtct tccgggtcaa ggtgatttaa taattgatgc
    10801 tcttaataaa agtaacacga gctttgatag gttaaaaaat ataataatta ctcatcatga
    10861 tatagaccat ataggaaaca taaattatct aagagaaaaa tctaagaata atatcaaagt
    10921 atatgcatac aaaagtgaag tcagttatat aacaggagaa gaaactcctt ttaaacttta
    10981 tatgttggaa caaatggtag ataaaattga tgataagatg cttagtatgc taaatgttat
    11041 ggtacttgga tttaaatcat catatactaa agtagatgtg tcattagata accatgaaaa
    11101 attgaatcta ggtgaggaaa tagaagttat acatacagga ggtcacacta gaggtcacat
    11161 atgtctttat ttaaaagagt ctaaagtact tattgctgga gatttgttac aggtagaaaa
    11221 tggagaactt acgcctgttg atgtaatgca cagcaacaaa caagagttaa aagatgctat
    11281 taaaaatata agtaattatg atatagaaac gatagttttt agtcatggtg gattatatca
    11341 aagagacata attggaacat tgaaaaacct aattattgag tagtactata tgatttacaa
    11401 taatttctta aattacttaa aattatatct aaagatgtag aatcaaaatt aaatataagt
    11461 aataagaatt ttgctttaaa attatactta agtgttaagt atgatacgta gtattacaag
    11521 tattttagaa tataattttt tatagtaatt ttaaggttct acaatacttc agttgaaaaa
    11581 tatttaacag catagtataa taggaaagaa aatattttaa ataaacttta gtatacaaag
    11641 tgaagaaaaa tatttaaatt atatataata ttgtagagtg aggtaaaata tttaagttat
    11701 atatcataat acagagtgaa gaaaaatatt tagataatgt accataatac aaggtgagta
    11761 aaaatattta aagcatgtat aataatgcaa agcgaagaaa aatatttaaa taatgtacca
    11821 taatgcagag tgagtaaaaa tatttaaata atgtgttata atacaaagcg aataagatta
    11881 tttaaagcat gtattataat ataaagtgag taaaaatatt taaatcatgt atcataatat
    11941 aagcagagtg aagaaaaata tttaaataat atatcataat gtagagtgag gtaaaatatt
    12001 taagttatgt atcataatac aaagtaaaga aaatcattta aattatatat cataaaaata
    12061 atatgaaata ttgaacactc ataatattgg aggctttaat attttaattg ataggttaca
    12121 ttatttataa cattaaaaat acaatttaca taaaatagac atatgaaaaa taaaaaatta
    12181 tataataatt atgggaatga ggttctccct tggatgataa tatcctaaac tgccaatgag
    12241 ctgatgactt ctatgatatc atagatgcca ccagcttttt ttgttgttta aatcgataaa
    12301 atggaggtga attacttact aaatttttat tttttaaatt tgaagaaata tttaattaat
    12361 agaaaaaaca gtagattata tgttaattat tttattaaga atgggttaag agtataccta
    12421 tatcctatca atttaaaaaa caattatgat agtcattaca ttgactacag ttgatgcaat
    12481 aaaactttca aaaaatctta tgatgcaaat agaaaggaat taaaatgata gaaaaattta
    12541 aaactttaat atggattaaa atgataataa gaattaaaag cgtttataaa acatatggtg
    12601 gactattttt gttaggactc ataggtatac cagttggtgc aattataggt cttatagaca
    12661 ccatatttgg aacggttctt ctaaaagtta cagatattcg agaaacatat ccaatgtatc
    12721 tgataccatt tttagcagta gtaggagttg ttattgctta ttgctatttt aaatttggag
    12781 gaaaaagtag taaaggaatg aatctaatat ttgaggtagg gcatggagaa gaagaaatta
    12841 ttcctttaag attggttcca tttataatat caggtacatg gttaacccat ttatttggtg
    12901 gtagtgcagg acgagaagga gttgctgttc agattggtgc aactttttca cattgggtag
    12961 gtaaaaggtt gcctataaaa aatgcttcta gtatttttct agtaactggt atagcagctg
    13021 gttttgcagg attatttgag acaccaatag cagcaatact atttgctatg gaggttttgg
    13081 tagctggttc actggagtat caatcgcttt ttcctgcgtt tactgcttct tttacagcaa
    13141 gtgcagtatc aaaaacttta gggttagaga aattttcttt tgcactttct tctaaagttg
    13201 tatttgactt accaatattt tggaagctta ttgttttagg aattattttt ggaatggttg
    13261 gtggagcatt tgcatggtgc ctgaaattgt caaaaagaaa aataggtaat agattaaaaa
    13321 atcctatgat aaggattgct attattggag tatgtcttag tgtattattc cttttatttt
    13381 ataaaggaag atattcaggt ttagggacaa atttaattca aaatagtttt tatggtggag
    13441 aaatttattc atttgactgg ctgttaaagt ttatacttac aatactgact ttgtcagcag
    13501 gttttcaagg aggagaggtt acacctttgt tttccattgg agctagtctt ggtgtattgt
    13561 tagcaggatt ttttaatttg ccaattgaat tagttgcagc gcttggctat gctagcgtct
    13621 ttggcagtgc tacaaatact ttttttgctc ctgtatttat aggggcagaa gtttttggat
    13681 acagctattt accttatttc tttgtagtat gcgctatttc atatatattt aatatggata
    13741 aatctattta ttcactacaa aaaatttcaa cgaaacaata aataaaacaa cataatgtat
    13801 gattataaga gaaagaatat tagagaaagg atagggtagg aatatgttga caagtttagc
    13861 tttgattttt ttattaggaa tggcctcggg aggtattttt gagaagataa aattacctag
    13921 cttattggga atgttactta caggaatcat attgggttca tatgttttaa atttgattga
    13981 taattcaata ctagatattt cttctgacct tagaaagatt gcattgatta ttatattaac
    14041 aagggctgga ttatctttag atataaacga tttgaagaaa gtgggaagac ctgctgtact
    14101 tatgtgcttt attccagcta cttttgaaat tatcggaatg atagttttag caccaaaatt
    14161 actaggcgtt tctatattgg aagcagcagt catgggtgca gtagtaggag cagtttctcc
    14221 cgcaattatt gtacctaaaa tgcttaaatt aatggaagaa ggttatggta cagaaaaaag
    14281 tattccacag atgcttttag caggaacttc tatagacgat attttcgtaa ttgtgatgtt
    14341 tacagtattc acaggattgg cacaaggaaa tagtatatca gctattagtt ttttacaaat
    14401 tccagtatca attatattag gtgtaatagt tggagcagtg attggaatct gtttagcagt
    14461 attttttaag aaggttcata tgagagattc tgctaaaggg gtgttgcttt taagtatctc
    14521 atttttaatg ataagtttgg agactgcttt agagggtatt gtgccatttt ctggattatt
    14581 agcagttatg aatattggga ttttccttca gataaaatat cgtgtagttg caagaagatt
    14641 gtctgtaaag tattcaaaac tatgggttgg tgctgaaatt ctcttgtttg ttttggtagg
    14701 ggcaacagta gatataagtt atgcatttaa agcaggaatt ggagctgtaa ttttaatatt
    14761 tggagttctt ttattccgta tggtaggagt attcttctgc cttattaaga caaatttgac
    14821 aataaaagaa agattgttct gtatgattgg ctatattcca aaggctacag tacaggcagc
    14881 aataggaggt gtaccacttg caatggggat agcatctgga caacttattt tgacattagc
    14941 ggttctcgct atacttataa ctgcacctct tggagctttt ggaattgatg taacttataa
    15001 aaaattatta acttctgtag aaggtaagag taagtaagaa taaaaataaa gattatttta
    15061 agtgataaat tatattttgg aaaaaactat attattgagt acaatgttag aaaatcattt
    15121 gagtgattac agaaggtaga agtttatatc ggattttaag gcatgtgatg attattagaa
    15181 ttgattttgt gaaattgtta ttgtaatatt taattatata gataaagaag cggagtgata
    15241 actatgtatt taaaaaaatt atcaaaatta gagttagtta ttatgaaatt tatttggaat
    15301 ttagatataa aaaccaattc atatgagatt attgattata tgaaagaaga gcataattta
    15361 ccagaaaaag tggctttaaa aattttatca aaattatcaa agaaaagatt cttatatgtt
    15421 caagaaacag gcaagtgtat gtattataca gttgcaataa aagaaaagac atatttagag
    15481 tttatatcaa gaaatgtgca aaatcttttg aagaataatt ttataaggaa tttattagta
    15541 tcatttcacg aagaggagct aacagaggaa aagataaagt cgttagaaaa ctgggtagta
    15601 aattgggaag aagcttatat ataaatgtta ttatttttat tacaatttag ataattttgg
    15661 ttttgtaagc tctgcaacta tttttagatg gttgcagagc ttatttttat tttttattcc
    15721 cttaagcaat acttaaaagt aaacgcattt atatttacta acttcaatca cttctaatac
    15781 ttagattact ttttaaaaat attttgatat gtcatctcta tagtaatcta gaacttgttt
    15841 ctattcttta tataaagttt ttatgaatga tatatataat attcgttcat ttga
//
LOCUS       DAELXX010000093        15552 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.093, whole genome shotgun sequence.
ACCESSION   DAELXX010000093 DAELXX010000000
VERSION     DAELXX010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15552
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.093"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            177..1568
                     /locus_tag="KPH29_002785"
     CDS             177..1568
                     /locus_tag="KPH29_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBG1468367.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
     gene            1821..3614
                     /locus_tag="KPH29_002786"
     CDS             1821..3614
                     /locus_tag="KPH29_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG1468368.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDGIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILDELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIVPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(3695..5077)
                     /locus_tag="KPH29_002787"
     CDS             complement(3695..5077)
                     /locus_tag="KPH29_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBG1468369.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGLSISMYGYRYVFLFSSITVLIGLFFFIFGNRKFYKPLN
                     NDPSNGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISFLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            5335..5682
                     /locus_tag="KPH29_002788"
     CDS             5335..5682
                     /locus_tag="KPH29_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBG1468370.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            5700..6734
                     /locus_tag="KPH29_002789"
     CDS             5700..6734
                     /locus_tag="KPH29_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468371.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVVWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(6783..7832)
                     /locus_tag="KPH29_002790"
     CDS             complement(6783..7832)
                     /locus_tag="KPH29_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG1468372.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENRVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            8107..9642
                     /locus_tag="KPH29_002791"
     CDS             8107..9642
                     /locus_tag="KPH29_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468373.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFNLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            complement(9828..10514)
                     /locus_tag="KPH29_002792"
     CDS             complement(9828..10514)
                     /locus_tag="KPH29_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HBG1468374.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEGVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
     gene            10639..12021
                     /locus_tag="KPH29_002793"
     CDS             10639..12021
                     /locus_tag="KPH29_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG1468375.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIEEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
     gene            12423..13691
                     /gene="eam"
                     /locus_tag="KPH29_002794"
     CDS             12423..13691
                     /gene="eam"
                     /locus_tag="KPH29_002794"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HBG1468376.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDICDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPVYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(13784..14140)
                     /locus_tag="KPH29_002795"
     CDS             complement(13784..14140)
                     /locus_tag="KPH29_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468377.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(14291..15385)
                     /locus_tag="KPH29_002796"
     CDS             complement(14291..15385)
                     /locus_tag="KPH29_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG1468378.1"
                     /translation="MQDKKIYLNIVLTVVVSYILIKVIDNYKYFFGVISLLMSLLTPF
                     IIAFVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADL
                     VAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFST
                     TFSVGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLN
                     IGKYFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTLMGVMNMVPYFGPVIGMAPVVI
                     INLFSNPTIALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIP
                     GMILSAPVMGVIKIYFCEFVNRRHDKIQME"
BASE COUNT         6034 a         1918 c         2497 g         5103 t
ORIGIN      
        1 aagatactaa aaaaataata tttttcataa ttttaaaaat aataataaaa aaatgaaaat
       61 acaggcgata atctattgag taaacataga aaagatgata ttataagtat gtgctaaaat
      121 ggttaaagca atattaatat taaattcaac tataaaacaa aagggggcgt ttttttatgt
      181 caagtacaga aagcgtatct aaaaaaagtg gtcatccgaa gagtttttgg cttatttgca
      241 tgacaatttt gtgggaaaga tttagttatt atggtttaac agctttattg gtattatttt
      301 ttacagcaag tgtagctcaa ggtggtttag gtctttctac tcaagaagca acatccatgt
      361 ttggcttatt tacaggtctt atatatctta caccattagt cggtggctgg ttagcagata
      421 aagttttagg tcaacaaaaa tgtatttcta taggtgcact tttaatagca ataggagatt
      481 tcgtactatt tgcttcatct acatctattg ccatggtttg ggtaggtcta tcaataatta
      541 ttttaggaaa tggttttttt aaatcaagtt gttcaaatat agttggggat atatatccta
      601 aaactgatgt gtcaagaaaa gatgcagcat acagtttatt ctatatggca gtaaatgtag
      661 gagcatttat agctccaata atatgtggat tgctttctga taatgtattt gcagtaagag
      721 gagcaagtgg agatatagta tcttatggat ataagatggc atttttaatt tgtggtatag
      781 gcatgatgtt aggtatgatt atatttacac ttcttgcacc aaaattatta aatgaagtcg
      841 gaaaatatcc tgtgactaaa ggtaataaag gtgaaaaaat tgagtatgga ccacttacta
      901 agttggagaa aaataggata atagcaatgg ttattttatt tttatttgtg gtcttattct
      961 ggacagcata ctatcaaaca caaagttctt ttatgattta tgtaagggat aatgtaaata
     1021 gaactctatt tggatttgaa atgccagtag catggcttac atcactaaat gctatattat
     1081 gtgtggcttt atgcccagta ttagctaccc tatgggttaa attatctcat accaaaagag
     1141 gtgatttctc tattccagtt aaaatgggat taggaatgat aataatgggt gttgggttta
     1201 tagttatggt atttgctaca ttagctaatg gtccagatgg aatggtaaag gctagtatat
     1261 tgtttatagt attgacttat atatttacaa cagtaggtga attattctta tctcctatag
     1321 gacttgcaat gttcaataaa ttagctccag ctaagtatgc tacattatct atgggagcct
     1381 ggtatctaac aagttttttt gcaagtttat tatcaggaaa gattgctggt tttacagcca
     1441 cacttggttt tttacagata tttggaggaa tagcaacagt tttaataata tttggtttaa
     1501 tattattggg aataagaaat gtattaacaa gattaatggc tatggattta ctaacaaaag
     1561 aaaaataatt catgacgaat tattgtaaat agatgaatta cttcaaataa aggaagttac
     1621 atttaaatat aaaattaaat gtaatttcct ctttgattat gaaaaaaagc tttacacaaa
     1681 tagatattat aattaaaatt ataattgtga taattagagt gtataagatt tatataaatt
     1741 atacaaaaaa tatttatgta ataaacttta catacttata tatttagtta ttcaaactat
     1801 tatttcgagg aggtcttgtt gtgaatataa aagatagatt aagtggatta agaaaattta
     1861 tggaagaaaa aaatatagat gcctatatga ttccatcttc tgataatcac caaagtgaat
     1921 atgtaggaga ttacttcaaa tcaagagaat ttatatcagg gtttaatggt tcagcaggga
     1981 cggttattgt gactaaagac gaagctgggt tatggaccga tggaagatat tttatacagg
     2041 cagaatctca attagaagga agtacaataa aattattcaa aatgggacaa gaaggttgcc
     2101 caactacaga tgaatattta tataaaaata ttcctgaagg aggaacttta ggatttgatg
     2161 gtagagttat atctgctaga gaaggagcta cattagctga aaaattatct aaaaaaggta
     2221 taaagataga atatcagtat gatttaatag atggtatatg gccagataga cctgctttat
     2281 cagatagtaa agcattttta ttagatgtta aatattgtgg agaaagtttt agctcaaaat
     2341 tagctagatt aagagaaaaa atgagtgaaa aaggtacttc tactcatgta ataactactc
     2401 ttgatgatat agcatggtta ttcaatataa gaggtggaga tgttaaatat aatccagttg
     2461 tactttctta tgcagtaata actttaaaag aagtttattt atttgttgat gaaagtaagt
     2521 taaatgaaga aatattagat gaactagcta aggaaaatgt tcaaataaag ccatataatg
     2581 atgtgtatga atttgtaaaa aatatagaca aaactgaaaa agttttatta gatggaacta
     2641 agcttagtta tacaatttac aataatatac cttgtgaagt tgagaaagtt gatgagttca
     2701 atccagtaat gttttttaaa gctcaaaaaa atgaggtaga attagaaaat ataagaaatt
     2761 cacatgtaaa agatggtgtg gctttcacta aatttatgta ctggttaaag aaaaatgtag
     2821 gtaagatgga aatcactgaa ataagtgcta ctcaaaaatt agaggactta agaagagagc
     2881 aagaaggttt ctttgagcca agtttcaata caattgctgc atataaagag catgctgcaa
     2941 tgatgcacta tagtgcaact ccagagtcaa attataaatt agaggcagaa ggattatttt
     3001 tagtagattc aggtggacaa tattatgatg gaactactga tataactagg actacagttt
     3061 taggacctat aagtgatgaa ttaaaactac actttacttc tgtagcaaga ggaatgataa
     3121 atctttctaa ggctaagttc ttgcatggtt gtagaggata taacttagac atactatcta
     3181 gaagttgcat gtggaatatg ggaattgatt atcaatgtgg aactggacat ggtataggat
     3241 ttgtgctaaa tgttcacgaa gctccaaatg gatttagatg gagagtagta cctgaaagat
     3301 ttgatagtgc agttcttgaa gaaggaatgg taactactaa tgaaccaggt atatatatag
     3361 aaggttctca tggtataaga acagagaatg aaattgtagt tagaaaagct gaaaagaatt
     3421 tctatggtca atttatggaa tttgaagttg ttacgttagc tcctattgac ttagatggta
     3481 tagtaccaga attaatgaat aaggatgaaa aagattattt aaactggtat cataagttag
     3541 tttatgataa aatatcacct ttcttaactg atgaagaaag agaatggtta aaagtttaca
     3601 ctagagctat atagtttgta ctagatacaa taaaaatcga ggtattataa tgcctcgatt
     3661 ttttattgta tattttatta actttaaaaa atttttaatt tgataattct actaattttt
     3721 tattcattat aagtagtata attccaaata tcaataaaaa tgatggtatc actatgaaga
     3781 cttgtaacat accaagttgg tctataatag cttgcgtata gccagataat ttatttgctg
     3841 caaatttgcc taaaaaccaa acacccatta ggaatgaaat atacttttca ggcgaaagtt
     3901 tatttaccat accatacccg actggagaaa agcaaatttc tcctatactt tggaatgtta
     3961 aaaatccaac tacccaaaat acacttgctt tgtgaattga attagggtct gttcctgttt
     4021 gtataactgc tactatcata aacaaaaatg ctatagctaa tagtataaaa cctaagctca
     4081 ttttttttgg cacacttaaa tcgccttttt tagtttgaga taattttacc catactaaag
     4141 cagagatagg tccaagaata acacaaagga gaccattgta tgaatctatc catgaagtag
     4201 gaatttgaaa attgccaaca gtaagattta tgtgttcctt tgtatagagt gcaatagaca
     4261 tagaggcttg gttatatgct atccaaaaaa atactgtgaa aaaagctaac accaatatta
     4321 ctattattct ttttttctca atcaaagtta aaggtcttga agcgagcatt ttcttatatt
     4381 ctagtgattt tcttatagac tctctcccgt tactagggtc attattcaaa ggtttataaa
     4441 actttctgtt tccaaatata aagaagaata aacctattag aaccgtaatt gatgaaaata
     4501 aaaatacata tctatatcca tacatactta tagataatcc gcttttcata gcaaaccatt
     4561 cattagcaac taaaccgcat attaaagggc caaataaagt acctagatta gtagccatat
     4621 acgtaataga gaaagctcca tcttttcttg ggtcatcatt ggtatataag tttccaacca
     4681 tagtatgtaa gtttccttta taaaaaccta ttcctaatcc agataaaaat atcatgatgt
     4741 ttatatccat cttactattt gctatgtatc ctagaaaata accaattgaa accattagca
     4801 ttccaagtat aatacagttt ttgggaccta aaaatctatc actgacccat ccaccaataa
     4861 ctggagagaa ataagtccat gctaaaaaag atgcaagcat tgatgcggat tctgctggtg
     4921 ttaaccctaa accacctttg gagacttgtt cagctaaaaa caataataat accaatttca
     4981 taccatagta tgcaagtcct acaaaggtaa atattgaaca gcagaaatat aaatctctag
     5041 gttgtttaag tttttcttgc gtttttaaaa ctgacatatg tcgagaacct ccagataaat
     5101 agtttaacaa aattttacca tactatatga gtatatgttt ttttcatata gacgtcaact
     5161 tgtaagataa tttaattaag tcgaaatata tgtaaaaata gtatcatttt tgtgtattca
     5221 gtaatatttt tttatgataa tatatgttat tattatatta aaatacaaac atatttatgg
     5281 aaaggatatg ccaatttagt agttagattg attgtttggc aggtgggatg agctatgata
     5341 agaaagaaat ctgaattaca agtagaagta aaagaaaatt taaatggtgg tataggaaag
     5401 gttaagcttg aaaacataat acaaaatgaa gagcttaaag gaaaaggaag actatttaaa
     5461 agagtagctt taccagtagg aagttcaatt ggtgtgcatg accatacaac agattttgaa
     5521 gtatattata tactaaaggg aaaaggtaaa gtatttgata atggggaatt tgtagaagta
     5581 aatgagggtg atgtagtgta tacagctgat ggcgaaaaac attctataga aaatatagga
     5641 gaagaagatc ttgagtttgt agcactagtt ttatatatgt aaaatatgga ggaggagtca
     5701 tgacaggcaa gatagaagaa attaaggaca agataaatag attttgcaac tccaatttaa
     5761 atcaagaata caaagatata agctttaaaa ttttgcaaaa ccttttagat aataataaag
     5821 agataattca ttcttctaga gctgatatat ggtcttcagc tatactaaat atagttttag
     5881 aacaaaatct tttatacaat aagaagcatc cactacatat aacaaaaaaa gagttttcaa
     5941 aacaaatagg tgtttcttta aatactataa ataataaatc gtctttaata aaagatgttt
     6001 gtagtattga ctttttaaat caagatacta tagctatttc taatgtagaa ttttgggtaa
     6061 aagagaccaa ttccaaatca aagtctgtag atttagattt agaaaagaaa aaaatactat
     6121 ataaaagata tattaagcta tcacaagatg cgaatagtga tattgaatca attagatata
     6181 tggaggaagc tgtaaatata ggtaaaagta tgattactaa agaagataaa gaacttggat
     6241 tttggaaagg tttatctacc agagcctata tgatagcact tgaatccctt gctagaaagt
     6301 tagaaagtat taataatcta aaggagtcca aaaaagtatt agaatattta atagaaatta
     6361 atcctagtga tgagcaggga ataagataca aattattaaa tgtattaatt agattaaatg
     6421 atagaacttc aataaataaa ttatttgaac tttataaaga agaaaaatct gctacatgga
     6481 tgtacagtaa agctctttat tactttaaaa ataaaagtat gtttttggca aatgattcta
     6541 ttaaggttgc tagaagtaaa aataagtacg ttggattata tttgttagat tggcgaaatg
     6601 cttttggtag agaatttgtt acagaggaag aaaaggcaga agctgtctat tactatgatg
     6661 aaaacatagt ggtttggaat gaagttaaag gttctatgga ttggctatta aaaaaaatgt
     6721 tggaattttc ataaaaaaaa ctactcctta cttttatata agtaaaagga gtagttttta
     6781 atttatacat ctagttttaa atcttcaggt tttattagac ctttttttct cataaattct
     6841 gaaatcaaca aagttacaat agcaggaagt ataaaattaa gtaaaagtat accaacccac
     6901 atagatatac ctccttttcc agcactttgc atagcagtta ctgttccaaa ttgtccaacc
     6961 aatccacaag tacccatacc agaaccaata ggaatattct ccattttgaa tattgttgta
     7021 gataatggtc caagtaatat tgaagctaca gtaggtggta tccaaatttt ccaatttttt
     7081 attatattag gtacttgcaa catagatgtc ccaagacctt gtgccacaag gccaccaact
     7141 ctattttctc taaagctcat aacagcaaag cctaccattt ggcaacaaca acctacagtt
     7201 gctgcaccac cagcaagacc acctaaactt aacatcatac aaatagctgc actacttata
     7261 ggtaatgtaa gagctattcc aacaagtgca gagactaaag ctcccattat tataggttgc
     7321 atagttgtag cttgcattat tatatttcca aatgaagtca taaaagcaga aacagctgga
     7381 ccaataagtt cagctacaag tactccaatt agtattgtca cagcaggtgt aaccaatata
     7441 tctatttttg tttcttttga aactaatttt ccaacctcta cagcaaaaag tgtagcgaca
     7501 aatgctccaa caggtcctcc taattcatta ccacaagctc caacaagtgc acaagaaaat
     7561 agtacaagtg gaggtgcttg aagtccatac gcaatagcca ctgctatagc tggaccagta
     7621 acctgtttag caagagggct tataacttca gtaaaaagag aaatattgaa attatctcct
     7681 aatgtattga aaattgttcc aattaaaagg gacgaaaaaa gaccaagtgc catataactt
     7741 agtgcgtcaa taagatacct ttgaacagaa atttcaatat tttttctttt agcaaatgat
     7801 ttgaaatttc tttttttata attgatttcc aatttaaaat cctcctcata taatataagt
     7861 ataatttctt tttaattaat tatataactt ttcagctgac aagacaaatg ttatttcact
     7921 aaaattagac tattgtaata gtaagaaatt aacattaatt ttagttttaa attttaaatt
     7981 tatggtttat aattagtata gggatattct gaaatataat aattttccag tttatcccaa
     8041 tatttaactt aggtagattg cttacaaaac aaatgattat atattaggct aaaaggaagt
     8101 ggatagatga tatttgtaaa aaataagaaa tacagaaaaa ttgccataat tatatctatt
     8161 cctatattgc ttgttgcgca gtactacctt tttaaatttg gaatacttag accaatggta
     8221 aagggagtag aaattgaaat tgtagatggg gaatatgtta aagatataga taaatatgta
     8281 gtaaaattaa atgaaacagt tactttgtct acaggtgaat atattaaggt tccttcgtat
     8341 gctaaaaatc caaatatttg gtttaatgta ttggataact cagggacttt aaaaatagat
     8401 ggaaataaaa tgacagcatt aaaggttggg tattcttctg tgggtatcat gaaaaattca
     8461 agagtattaa aaaaggctac tataaaagta gtagacccag aggtagaatc actagatatg
     8521 gagattaatg gggatttaaa acatgttggt gatagtgcta ctatagaaag tacaatagaa
     8581 gttgattatg ataagtttaa aaaatcatat aagccagttt ataagagtag caatgaaaat
     8641 attttaaaaa tcaaaggaaa taaagtaagt gcagttggag ttggtaaagc tacaatatat
     8701 gctaagtgtg gaaataaaga aattgagaga acatttagga ttcaagctag agttgctgaa
     8761 ataaatatgg atgatataaa aatagaagtt gaccaaaatg aaaaattgaa accagatata
     8821 ataacttctc cacctggttt agaaccacca aaaatatatt atgagttagt agattcaaaa
     8881 ttacctgtag aaagagcaat aagtttaagt agtaatggaa atgttgtagg tcttagagaa
     8941 ggtaaagata gggtgagaat aacttgtgga gataaatcta aagtaataac agtaacagta
     9001 gtcaaagaaa ctataacgaa tgactatata caaaacttgg ttggttcaca taaaatagta
     9061 aacaataaag ttattattaa acttgaatgg gattatatga aagatgtctt tgattatgat
     9121 atatatctta aagataagtt gtcaggagaa tcatttaaga aaattaaatc tataactata
     9181 aatgaaagtg atttaggtaa gtctaacaag gtatatgcaa ctatagaagt agatatgaag
     9241 ggtaatcaaa atatagattt tgatatttat gtcataggga aaaaggattc agatacaact
     9301 aagccaagta atgtaataaa tattactaat ggaaatggaa attcaaataa tgataataat
     9361 attaaagata tgagggtaga aaatatatca gctaatgttg atagagataa taatgtcatt
     9421 agagttaact ggagtagtat atctcataag gactgtactt acagtatata tgtaaaaaat
     9481 aatactaatg gagatggaga atttaatcta tatcaaagtg atatacaggg aaatgagcat
     9541 actataagta ttcctgaagg agatttaaat ctagatattt atgttgttgc aaattataat
     9601 ggaaatagct ctcaaaatag tgatgttata aatgtaagat agatttgtat acaaattgaa
     9661 agtgttgagt ttatttaaat attaaattaa gaaaaaggat agtatagggt aattatacta
     9721 tccttttttt gttacaatat ataggtttaa aatctctagt aatataaatt ataaaaagtc
     9781 ttatttacac aaatctatag atattttttt tattaatcaa tattgtctta aattgaactt
     9841 agtgcggctt ctattgcatg tattactgtc gtatcaaata gtggaataga agtgtcattt
     9901 tgtttaatta aaagacctat ttctgtacat cctaatataa caccctctgc accttgtttg
     9961 ccaagcttat ctataataga taagaaagct ttttttgaag attcagatat aattcctaag
    10021 caaagttcgt taaatattgt gtcatttact attatacggt cttcttcatt tggaattaag
    10081 acttcaattc cattattaat aattacattt ttataaaaat cttgttccat tgtatattta
    10141 gtacctaata aggccacttt ttttatttct ttttccctta atactgtagc agttacatct
    10201 gctatgtgaa gtaatggaat atgtatactt tcttgtattt tatctgatac cttatgcata
    10261 gtattagtac agataacaat aaaatctgcc cctgcctctt gtaatttaat tgcagcatca
    10321 gctaaaattt tggcgctttt ttcccaattt ccactagatt ggcattcttc aatttcttga
    10381 aaatctacac tgtaaagtat acatttagaa gagtgtagtc cacctaatct ttcttttatt
    10441 actgtattaa taacttgata atatgtaatg gtactttccc aactcatacc accaattaag
    10501 cctatagtct tcatattata cctctcttat ttatttattt tgaaatagct atttacaaat
    10561 acaagtatag catctaaaat agaagcatac aataataaaa ttgtatggag tacaattata
    10621 attaagagga gtaattatat gccaataaat tcttttgaaa actatcctat gacatggaaa
    10681 ccaaaattag ataaccgaaa accaccaata tataaaactt tagcaatgtt attagaagaa
    10741 gatattaaaa gaggaaattt aaatcctgga gataaactgc ctccacaacg ggaattagca
    10801 gattttctag atttaaactt aagtacaata acacgagcat ttaaactttg cgaagaaaag
    10861 ggactaatat gtgcaaaagt gggaaaggga acattcattt cttctgatgt aaatgtaagt
    10921 aatacattat tatatcaaac ggaatcaaaa gacataattg aattagggac agttcatcct
    10981 ccatatgagc aaaatacata tattattgac tttattaaaa atgtactaaa acaaccagaa
    11041 atggataggt ttttacaata catgtctcca tctggcacat atatgcaaaa aaagtcaatt
    11101 gcaaaatgga tagaaagaaa taatgtatac accagtgaag aaaatatatt attatctaca
    11161 ggagggcaaa atgctatatg tgcaacttta ctaggattat ttaaagctgg tgatagaatt
    11221 gctacagatt ctttaagttt ttctggaatt aagtcaatag ctaaaatgat aggtattcaa
    11281 ttagtcccaa ttcctcaaga aaataatgaa atatcgattg aatatcttga aagttattgt
    11341 aaaaatgaaa atataaaagg catatatctt ataccagatt atcataatcc aacgacacat
    11401 actatgtctg atttatctcg taaaaagata gccaagatag caaagcaata taatataata
    11461 attattgaag atgctataaa tagcattttt agagacggta tacaaactcc catattttct
    11521 ttagctagtg ataatactat atatattttt agtacatcta aatttttatg tgctggtctt
    11581 agagtagcat ttgtagttgc tccaaaacga tatatagaaa atttagaaaa tgcactatat
    11641 aatatgaatc taatggtttc tccatttaca gcagaaatag tacatcgttt attatattct
    11701 cctattatag ataaaattat agaagagaaa agagatgcaa ttatagaaag aaatgaagtt
    11761 gctgataaag ttttgtcaga ttataatttg attggtgata agaattgcag ttttcgttgg
    11821 ttacttcttc ctgatgaatt ggatggaaaa tcatttgaaa tttctgcaaa aaatctagga
    11881 gttcaagtat attgtgctga acgattttca gtaggaaact ctacagttcc aaaagcagtt
    11941 cgtatttgtg taacagctcc aaaagatgta gaagaattag aaaaagggct taatataata
    12001 aagtcacttc ttaagagtta aatttatttt actattattt aattaaataa ataaaaaggc
    12061 ggagaaattt attataataa acctccgctt ttattattta atgaaatact ttacttttta
    12121 ttaaaaaatt taattttaat aatcttgaaa atataatcgt tttatggtaa aataattctt
    12181 aaaaatagaa aaaattaatc atttgttaaa aaaaatgcat aatttattgt aatttattga
    12241 cgaaggttga aaaatattca taaatgtgcg aaataacaga aaaaatgcaa aaattaattc
    12301 aatttcattt gttataaaat ctatatggca aaattaaatc tgcctatcag aatgatataa
    12361 acacatcaga aaggaggacg ctatcaaata atacaagtag ttaactttga tagcaaaaaa
    12421 ttatgaatga acaaactaga atatccttag agagagctgc tgaattaaaa tcaaaaattg
    12481 atgattatat tcaggctaga aaaacgatta acagaggtct tgaaaaagaa gaagagataa
    12541 ataaacgaaa acagaaaata ttaagtatct taaatggaac tgaagaggat tggaataact
    12601 acaaatggca attatcaaat agaataacag atgtagatac tttatcaaaa attataactc
    12661 taactaaaaa agaaaaagaa tatataaaag aggttggtac tcaatttaga tgggcaatat
    12721 ctccatatta tttgagtctt atagacccag aagatatatg tgacccaata aaattactgt
    12781 ctataccaac acatatagag ttggaagatg agcaagaaga tttggaccca atgggagaag
    12841 agtatacaaa cccagcagga tgtataacta gaagataccc agatagatta ataataaatg
    12901 taacaaatga gtgtgctatg tattgtagac actgtcagag aagaagaaat attggacaac
    12961 aagattctca taagtcaaaa gctattatcc aagaatctat agactatatc agagaaaatg
    13021 aagaaataag agatgtacta gtaactggtg gagatgctct tacattaaaa gatgattatt
    13081 tagagtggat tcttagccaa cttaaagaga taccacatgt tgattatgtt agattaggta
    13141 ctaggactct tgttacaatg ccacaaagaa ttacagatga attttgcaat atgctaaaaa
    13201 aatatcaccc tgtatatata aatactcatt ttaatcatcc aatggaaata actaaggaat
    13261 ctaaagaagc ttgtgaaaag ttagcaaatg caggagttcc attaggaaat caggcagtat
    13321 tattaaatgg aataaataat gataaatttg taatgagatg tttaaatcaa gaattactaa
    13381 aaataagagt aaaaccttat tatatattcc aaagtaaaca tgtaaaggga acaaaacatt
    13441 tcaatacatc agtagatgat ggtcttgaaa tcatggagta tttaagagga tatacatcag
    13501 gaatggctat accaacatat atagtaaatg cgccaaaagg aggaggaaag actcctttgc
    13561 ttccgcaata ccttgtatca aaaggaacag attacgttat gcttagaaca tgggaaggaa
    13621 aagttataaa aatggaagat gaacctgctg tagatataaa gaaacttata aaagagcaag
    13681 cacaggatta aaaaagtatt taaagtttat aaaaagaaac aactttaaaa taaaaagaaa
    13741 atggatgtca aatttatatc aatttgcatc cattttttat tagttattta ttattttgtt
    13801 gttgattaac taattcaaga tatccacttg ggtggaagta atatatctca gaagtatttt
    13861 catttacaaa gtaaccaata tcaccatctg catctggtaa aaatacataa ccattaagat
    13921 ttttagcttt taatgcttca aaatcactag catttccttg atatatgtaa tcaacttcat
    13981 tgttgtattt tactaaaagt tctttagctt gttcttgagt caatttagta ttttggctta
    14041 caggaacatt cttttgagaa tctgcataag aagattcatt tactggtgca gttaataaca
    14101 aacaagataa aactaataaa gatataagtg cttttttcat actaaaaccc tctttcatgt
    14161 ttaattatat aagattcatt acttaataat attgtatgtt tcctataatt aaatattacc
    14221 attaaagaga ataatattac agttacacag ctgtaacttt tgattacaaa atcgtaatta
    14281 aattgtattt ttattccatt tgtattttat catgtctacg atttacaaac tcacagaagt
    14341 atatctttat aacgcccatc acaggagcag atagaatcat accaggtata ccaaagaatc
    14401 ctccaccaac agtaactgct aggattgtaa agaatgggct aagacctagt tgtccaccta
    14461 cgatttttgg ctctataaat gtaacttcaa cttgttgaat tattattaag taaattaaag
    14521 aagttaaagc aattgttgga ttggagaata aattaattat tacaactggt gccataccta
    14581 ttactggacc aaaatatgga accatattca tgacacccat aagtgtacca aataatagtg
    14641 catattcaga ttttaaaaag taaagaccaa ttccagaaag tactccaact ataaaagaat
    14701 ctaaaatctt acctgtaaag tatttaccaa tatttaaatt taagctttga catagttcta
    14761 ttataaagtc tccatatttt ttacctaggg aaatataaac tacttttttt gtaaacagta
    14821 agaatttttc tttttctaat aatatataga aacaaattat aaaacctaaa agaaattgta
    14881 ctataaattt tccaactgaa aatgtagtgc taaatatccc accaagtgaa ccaataagca
    14941 agttactaaa tttaggcatt acactcataa ttttgtcacc atactctttt aatgtattag
    15001 ggtcaactga ttgtaaactc ttacctaagt cgaataagaa ctgctctgtt ttatctatgt
    15061 atgtaggaat ttgagcaact aaatcagcta aactatttac tatggatgga actgtaaata
    15121 atataaagga aattagtata actatgagta caccataagt aagaagtaac gaatatattc
    15181 tcttaaaatt taatttactt tctaagaact ttactagtgg gttgaatata tatgctaata
    15241 caaatgctat tataaatggt gttagtagtg acattaataa acttatcact ccaaaaaaat
    15301 atttgtaatt atcaataact ttaatcaaga tatatgaaac aactacagtg agtacaatat
    15361 ttagataaat ttttttatct tgcaaaataa cacaaccttt cttatattta ttaaatttag
    15421 atatacttaa atctaagtta aagttaatat ttatagctta ttatacaata aaaagcagta
    15481 ttaatatagt atgattaata tgaaattata aatacgttaa aactttatgt ttatagctaa
    15541 aataataaaa at
//
LOCUS       DAELXX010000094        15508 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.094, whole genome shotgun sequence.
ACCESSION   DAELXX010000094 DAELXX010000000
VERSION     DAELXX010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15508
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.094"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(71..1147)
                     /locus_tag="KPH29_002797"
     CDS             complement(71..1147)
                     /locus_tag="KPH29_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBG1468379.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
     gene            complement(1520..2035)
                     /locus_tag="KPH29_002798"
     CDS             complement(1520..2035)
                     /locus_tag="KPH29_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HBG1468380.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(2398..3468)
                     /gene="leuB"
                     /locus_tag="KPH29_002799"
     CDS             complement(2398..3468)
                     /gene="leuB"
                     /locus_tag="KPH29_002799"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBG1468381.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDEIGEPLPNKTVETCRKNEAVLLGAVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTRAMGELIVNEI"
     gene            complement(3475..3966)
                     /gene="leuD"
                     /locus_tag="KPH29_002800"
     CDS             complement(3475..3966)
                     /gene="leuD"
                     /locus_tag="KPH29_002800"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBG1468382.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKEKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(3981..5258)
                     /gene="leuC"
                     /locus_tag="KPH29_002801"
     CDS             complement(3981..5258)
                     /gene="leuC"
                     /locus_tag="KPH29_002801"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBG1468383.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTITKDVNIYEADEDAVYDEVYEIDLAELKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            complement(5215..6957)
                     /gene="leuA"
                     /locus_tag="KPH29_002802"
     CDS             complement(5215..6957)
                     /gene="leuA"
                     /locus_tag="KPH29_002802"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBG1468384.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIKGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTPQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     misc_binding    complement(7081..7327)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7475..7921)
                     /locus_tag="KPH29_002803"
     CDS             complement(7475..7921)
                     /locus_tag="KPH29_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468385.1"
                     /translation="MSKNKFIYMNYYDNGYFKKGMTIYMVLALIILATIFIYSTTILY
                     ILCIITAVFLFKQGRDFSNKYEGKVYITLDNHSILINNQCIFSIKSKQSQKFNYKIIE
                     KIEVVKNILNIYTDESNYKIRLRALSLEDEKKLLNIIDEKMKKFKA"
     gene            complement(7942..8661)
                     /locus_tag="KPH29_002804"
     CDS             complement(7942..8661)
                     /locus_tag="KPH29_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBG1468386.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYSGNLDVQEDMLQHLDYIIASL
                     HRPCIASGTKEENTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGTRVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            complement(8779..10053)
                     /locus_tag="KPH29_002805"
     CDS             complement(8779..10053)
                     /locus_tag="KPH29_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBG1468387.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAELQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNAKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
     gene            complement(10253..11620)
                     /locus_tag="KPH29_002806"
     CDS             complement(10253..11620)
                     /locus_tag="KPH29_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG1468388.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIRYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIKE"
     gene            complement(11870..12334)
                     /locus_tag="KPH29_002807"
     CDS             complement(11870..12334)
                     /locus_tag="KPH29_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HBG1468389.1"
                     /translation="MSLRIGGNSLGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVY
                     VFSVIGSTLVSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGII
                     LFVGIPLPTTGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            complement(12339..12968)
                     /locus_tag="KPH29_002808"
     CDS             complement(12339..12968)
                     /locus_tag="KPH29_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBG1468390.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRMIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADKVIGTEFFYENSRYKNQFIGENCKGIEKVKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            complement(13002..13889)
                     /locus_tag="KPH29_002809"
     CDS             complement(13002..13889)
                     /locus_tag="KPH29_002809"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HBG1468391.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYSLYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            complement(13918..15156)
                     /locus_tag="KPH29_002810"
     CDS             complement(13918..15156)
                     /locus_tag="KPH29_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468392.1"
                     /translation="MDGDILMSKILKYKNEMFLFILVVITYLIITKIFFSKTTIFYNL
                     NNTYDVLLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTD
                     FNSAYGFGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNV
                     ERFIYSQFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKI
                     GKMLKHLITFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLL
                     YSVFYFPGASIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIII
                     FNLTLHGIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIV
                     ISNVNGFIEILNIGIKSYPV"
BASE COUNT         5059 a         2796 c         1626 g         6027 t
ORIGIN      
        1 tgtaattttt ataaaatatt atttattata tattctaaat ttatcttaaa acatacatat
       61 ttttacaata ttatttatta aaacctaata tatcatcaat attatctaat aattcttgta
      121 catcttcgac agtatagtca cccatatgtg aaattctaaa agttttttct tttaaatctc
      181 catagccatt tgctatttgg aatcctcgtt cttgtaattt tgaatttaaa tttgatacac
      241 taataccttg agtattttct ataacagtaa gtgtattgga taagtgattt tcattggtaa
      301 aaatttggaa gtgtttttta gcccagtttc taactaagtt tgccatgtct tcatgtctgt
      361 tgaatctgtt atctaggcct tcatctaata tattatctaa ttggaaatct aaagcgaaca
      421 tgtgagaaag agatggtgtt gaagggtatt ggtagttttt cttgattaag tattcataca
      481 ttgctaacaa atcaagatat actcctctaa aaggtacttg cttagctcta tcgattgctt
      541 tttgagaaaa tgtgcaaata gacattcctg gtggtaatcc cagtgctttt tgtactgatg
      601 ttatacaaat atctattcca atcttatcaa cctcgacttt tattccaccg gctgaactta
      661 cagtatctac acagtagatt acatcttcat actttttaac aacttctcca atttcttcta
      721 ttggatttct tattccagtt gaagtttcat tgtgagttac agttattaaa tcgtattctc
      781 cggtagaaag caccttatca accatttctg gcgttgttgc ctctcctaat tcaactttaa
      841 atatatccgc tgggacatta ttagccactg ccattttgta ccatctatca ccaaaggatc
      901 cacaggaaaa tactgctgcc tttttggatg tacaggaacg aatggagcct tccattaaac
      961 cagttccgga tgacgtggat aaaagtatcg tgttgtttgt atagaatagt ttttgcatct
     1021 tttcacttat actcttttgg agcatagatg catcttttcc tctatgtcct atcattggtg
     1081 tagccatctt ttgcaagact tcttctttaa catcaatagg accaggaata aataatttct
     1141 tactcattat aattcccccc tttttagttt gtaatatgga tagcgttttt actgtcctta
     1201 tttttaataa ctataatatc aagctgaatt aactatgtca acataaaatt tattttttat
     1261 atataattta ttttatatat tttatgaaaa cattcatttt ctgccttttc ataagtctga
     1321 attttctgaa ttattctctc aaaaaaaata tactttttat ctatataaat taattttttt
     1381 agtatgtaaa tttgacgcta ttacgataaa tctttttagt ataactaatt tatttttatc
     1441 ttgtattttt attgtatttt tgaattttat tttttctaaa tataaaattt atgttttaaa
     1501 atataaaatt tttatttaat caaagttcaa tatattccac tttttctttc gattcttcaa
     1561 aaaaccattc ttcattagaa aaatcacctt ctgcaatttg tccaataggc attttcatat
     1621 actttccagt tgctgtcact gctatatctc catttggtag aattatttct cctgtacctt
     1681 caaataattt tctgttttct ttagttattc ttccaacaac ttttatagta gcatctgttg
     1741 gtactggttt cttatatttc aactctaaag atactgttac tccccatatt tggtcatcat
     1801 ttatacttac agctcttcca atagtctcat ctaatattgc agcagacata cctccatgaa
     1861 ctctcccagg ataactttga tgccaatcct ttgtattaca aattgataca agctctccat
     1921 tttcaagttc ataaaaatct gctttaagtc ctagctcatt ttgtgttcca caaactaaac
     1981 aagatttact actattttgc ttcttcaaaa ctttatactt tgccaaaaaa tccatctcct
     2041 aaccgacatt agtagtcgct taatattaaa taattctaaa atatattata taatattaat
     2101 aaaatccctg caaacttcta ataataaaaa taggctactt caatatgata agtttaattt
     2161 aaagtattat tttccaaaaa ataactttat caaaatagaa actcaaagta gatataagcc
     2221 tctttttcgc tattaaaata gtaaaaatat atttgactta atataaaaaa gagtgtctca
     2281 ttaactaata aagtttattt tgagacaccc ctcataaaac aaattatact acaatagatt
     2341 tttactaatt ctacaagtta tatatttaaa ttatattatt caaattaaac tataattcta
     2401 aatttcattt actattaatt cgcccattgc tcttgttcct acaacatttc ccacaccatt
     2461 atagatatca atagttctat aacctttatt gagtacactt tgtacagcat cttcaataca
     2521 cttagcttct tcttctaagt taaaactatg tcttaacatc atgcttacag atagaatagt
     2581 tgcaattgga tttacaatat cttttccagc tatatcaggc gcacttccat gtattggttc
     2641 atacatacca aaactatctt ctctaagtga tgctgatggt aacataccta tagaacccgt
     2701 aatcatagat gcttcatcag atattatatc tccaaatata ttattagtca ctattacatc
     2761 aaattggcta gggtctttta caacttgcat agcagcatta tcaacataca tatgtgacag
     2821 ttctacatcc tcaaactctt tagctaagtc ttccattata cttctccaaa gtcttgaact
     2881 ctcaagcaca tttgccttat ctacacttat aagtttttta tttctatttc tggctatttc
     2941 aaaagctctc ttaccaattc ttcttatttc attttcgtta tatatttcta catcataagc
     3001 attttcgata ccatcaatga tttttcttcc acgttctcca aaataaatgc cccctgttaa
     3061 ttctcttaca acaacaaaat caatgccttt ctcaactata tccgttctaa gtggtgaagc
     3121 ttcttttata ctttcataca ttgtagctgg tcttaaattt gcaaataaac ccaatgactc
     3181 tcttaatttt aaaagaccag actctggtct tttatctccc ttacatttat cccactttgg
     3241 tcctccaaca gcaccaagca atacagcttc gttttttcga caagtttcaa cagttttatt
     3301 aggtagaggt tctccaattt catcaatcgc gcatcctcca gctaatacat attcacaatc
     3361 gaatctatga ttaaatttac aacaaatttt atttagaacc tttatacctt catctataat
     3421 ttccggtcca acaccatcac cttttatgac tgctatatta caattcatac tccactaccc
     3481 cctatttgct tttattgagt taattaagcc ttcatttaat ataatatttt gcataaactc
     3541 aggaaatggt tctgctttat attctttttc ttttgttata ttttttatca cacctgtaga
     3601 aaaatctact tctatattat ccccttcatc tatgttattg gcagcttctt cacattcaag
     3661 tataggaagc cctatattta tagagtttct aaaaaatatt ctagcaaaag tagaagcaat
     3721 cacacaggat actccacttt cttttattac aattggtgca tgttctcttg atgaaccaca
     3781 accaaagttt ttagttgcaa ctataatatc tccttttttt accttagaaa taaatttatc
     3841 atcaatatcc tccatacaat gcgttgctag ttctttataa tcagctatat ttaaatatct
     3901 agctggtatt attacatctg tatctatatt gtcaccgaat ttaaaaacac ttccattggc
     3961 aatcataaat tttacctccc ttaaattatt tctgatggtt ttgatatttt tccagttata
     4021 gctgatgctg ctgctacagc tgggcttgca agatacacct ctgaatcaac atgccccatt
     4081 cttcctacga aattcctatt tgttgttgaa atggattttt ctcctgctgc aagtataccc
     4141 atatgtcccc ctagacatgg accacaagtt ggtgtagaaa ctgctgctcc tgcctctata
     4201 aaagtggtaa tatacccttt ttctatagct tcaagatata ctttttgagt tccaggaaat
     4261 atcataactc taactccatc tgctactttt ttgcctttca ttatctcagc tactacttca
     4321 aggtctgata ttcttccatt tgtacaagaa cctattacaa cttggtctat ctttatatcc
     4381 ttatctatct catctactgt tctagtattt tcaggaagat gaggaaaggc tactgtttct
     4441 tttagttcgg ctaaatctat ttcgtatact tcatcataga ctgcatcttc atctgcttca
     4501 tatatattta catcttttgt tatagttgaa cacttatgtg aatttatata ttccatagtc
     4561 ttatcgtcta ctggaaagat tccatttttt gctcctgctt ctattgccat attacaaata
     4621 gtaaatctat catccataga taagtattct actccatctc cacaaaattc catcgacttg
     4681 taaagtgctc catcaacacc tatctcccct attatatgaa gtattatatc tttaccactt
     4741 atccatttat ttggtttatt cttcaaaaca aattttattg ctccaggaac cttaaaccaa
     4801 cattttcctg tagccatacc tgctcccata tcagttgaac ctacaccagt agaaaatgct
     4861 cctaatgctc catatgtgca agtatgagag tcagcaccta taattacatc tccagcagtt
     4921 actatacctt tttcaggaag aagaacatgc tctattccca tatctcctac atcaaaaaaa
     4981 tttacaactc cttttgattt tgcgaaactt cttgtaaatt tacattgttc tgcactcttt
     5041 atatctttat taggtgtaaa gtggtctaaa accatcacta ctttagtctt atcataaaca
     5101 tcttcaattc ctaatttttc aaattctcta atggctactg gtgatgttat atcattacct
     5161 aaaactacat ctaaatctgc ttcgattaat tgccctttct taacttcatt taatttagca
     5221 tgtttggcta atattttctg cgtcatagtc atccccattt tttatttcct cctcaatata
     5281 acttttattt attgcattca taagtgcatt aatagctgct gtatttatgt tttccgatat
     5341 acttacacca aaatagactt cattattgtg ctctattcct acataagcta cccctcttga
     5401 atgtgaccct tcctctaatg catgttccca gtaatatcta aatttataat ctttcatgcc
     5461 acattgcttt agtgcattat taaatgaatc tacaggtcca tttcctatac caacaatcct
     5521 ttgttcattt cccatataac ttattcttgc agtcatctca atcatgttag tatcattaga
     5581 atcatcattt tcatagttta attcatccat tgattttatt ttatacttct taactttata
     5641 agggctctca acttcaatat attctttctt aaataaatta aatattgctt cactactaag
     5701 ttcttcccca attctatcag attccatttt gactacattt ccaaaatcag aatgcatatt
     5761 cttaggtatc ataaatccat aatctgtttc cattatataa acagctcctc cttttccaga
     5821 ttggctattt atccttataa tttctttgta ttctcttcca atatcatgag ggtctattgg
     5881 taaatatgga acttcccagt aatcattatc tctgtttttc attgctatca tgccttttct
     5941 aatagcatct tgatgtgaac ctgaaaaagc acaatgtaca agattacctg catatggatg
     6001 tctatcatgt accataagtt ttgtacagtc ttcatatata ccaattatct tatctatatt
     6061 gctaaaatct agctctgggt ctattccttg tgtataaaga ttaagtccca catttactat
     6121 atccatattt ccagttcttt caccatttcc aaatagtgtt ccttcaagtc tatcggcacc
     6181 agccaataat ccaagttcac ttgcagccgt acaagttcct ctatcattgt gagtatgcaa
     6241 acttagtata atagaatccc tacaacttat atttttacaa aaccattcta tttggtctgc
     6301 atatacgttt ggtgttgcca tttccactgt tgatggaaga tttattatta cttttttttg
     6361 aggagttggt ttccaaacat ctatcactgc ctcacatatt tcaagtgcat aatccatctc
     6421 tgttcctgta aaactctctg gtgaatattc aaaagtaaat tctgtttctg gatatttttt
     6481 tacttcttca ttaaataatt ttgctccttc aactgctata tctattattt cttgtttact
     6541 catattaaaa accacatctc tttgaagaac agatgtagag ttatataaat gtataattgc
     6601 ttttttacat ccctttattg attcaaatgt tttttctata agatgagccc tagattgtgt
     6661 aagtacttgt atagttacat catctggaat catattctca tctattaatt ttcttaaaaa
     6721 tgtatactct gtatttgatg ctgatggaaa tcctacctct atttctttaa atcctgtatc
     6781 aataagcatt ttgaacattc tgactttttc atttacactc ataggcgttg gcagtgactg
     6841 atttccgtct cttaaatcga cactacacca aattggagcc ttaaatattt cattatctgg
     6901 ccatttacga tttactattt gcattttatc atatttttta tactttccac acttcatgtt
     6961 atttccccct ttttctgttt tttgtattca gtatacctta tatcgacgca atcctgcaca
     7021 cttcataagg ataaatgtca attttatcat tagaataaaa aaaacctcat ctctaataaa
     7081 attagagacg aggttttgct cgcggtacca ctctatttag ctttctaaaa agctccctta
     7141 gtaatagcca atacaataat tataatgact atatttccta taacgtggaa ttactccgat
     7201 tattctaaga tttaaatctt agaaaacagc tcctagacaa gttcagaact atcaactact
     7261 aaatcacacc atcctttagc tctctgaaag tttcattgtt cttactactt ctattcatca
     7321 cttttttaat catttaaatt ttctaaatat tttgatttaa tagttattat atacacatta
     7381 aaatgtattg tcaacaaaaa aaataattat ctcaattttt tatttttgat aagcaattct
     7441 ataaaacatg ctaactattg aaaacagtat gatattatgc cttaaacttt ttcatctttt
     7501 catctatgat attcaataat tttttttcat cttccaaact caatgccctt aatctaattt
     7561 tataattaga ttcgtcagtg tatatattta atatattttt tactacttct attttttcta
     7621 ttatcttata attaaacttt tgactttgtt tagattttat actgaatata cattgattat
     7681 ttattaatat tgaatgatta tcgagagtaa tatatacttt tccctcatat ttatttgaaa
     7741 aatcacgacc ttgtttaaat agaaaaactg ctgttattat acataaaata tataatattg
     7801 tcgttgaata tataaaaatt gtagctaaaa ttataagtgc taaaaccata tatatagtca
     7861 ttcctttttt aaaataacca ttgtcataat aattcatata gataaactta tttttactca
     7921 aattttttca ctcccaagat tctaaaaatt tatatcaaaa aattctataa tttcatcttc
     7981 atgataattt atgactaatt catctggaaa atctactgct tctaaaactt tttctgcatt
     8041 ttcaaattct cctatagaat aacatatatg agagtcagta ccaagaatta ctcttgtacc
     8101 atatgtttta cacaatgtca acatatgact tacattttcc caagtacccg ttctatgaga
     8161 gtttgaactt aatgatgaat tatttatttc taaaaggata tttttatctt tagctttttt
     8221 tactataggt tcataatcta aaggatacct actgtcatct ggatgtccaa ttatttttac
     8281 cttaggttta tccataactt ttaaaatagc atttgtattt tcttcttttg taccagaagc
     8341 tatacaaggt ctatgcaaac ttgcgattat ataatccaaa tgttgcaaca tatcttcttg
     8401 aacatctaaa ttaccactat aatccattat atttccttct attcccctaa gaatctttac
     8461 accttgcact tcacgtggaa gcaaatgcaa attataaaaa tagaatggat gtggtcctcc
     8521 tggcatatta ggtccgtggt ctgatattcc taagtatttt atattcttct tactcgcctc
     8581 ttcgacattt tccttgaaag tactatgtgc atgtccactt attagagtgt gacaatgtac
     8641 atccataata gactttttca ttaaatttcc tcctaatatt ttacataaat tcgtgaaaat
     8701 agctaaaggg ctatttcaaa actaaagctg tcataataga cagtgtaacg ttttaaaaca
     8761 gccccatttt caattatttt acacttctaa atctttaaat ttatctttaa ataattctcc
     8821 aatacttcct aaagattcct ctacttctag gtattctgtt atatcctctt ctggctctct
     8881 agttgcttct tttattgaaa ccaacattct tttagtcttt ggtttaaaat caagaatctt
     8941 aactttaacc tcatctccaa tattaacaac attagtaact ttagccactc tagtatcact
     9001 caattcagat attggtaaat acgcatctac accttcttta atttgaacaa aagccccttt
     9061 ttctattatt ctaagaactt tagcattgac aacatcatca atttgtactt cttttgctat
     9121 taaatcccaa gggtcattat ttatgtcttt taatgccaag gcaatttttt tagtctcttt
     9181 atttacatcc aacacataaa cttctacttc ttgtccctct gaaacaacat cttcaacact
     9241 ttcaactctt gtccaagcta aattatttat atgtgctaat ccttcaatcc caccaatatc
     9301 cacaaatgca ccataaggca ttatctttgt caccttacca tctctttttt gtccaacttc
     9361 aagagaatta aataagtctt ctttagctgc tttgatacga gctttttctt cttctctttc
     9421 ttgcttaatt cttgctcttt cagcttcttt ttctgcctta atttgagctc ttctttcttc
     9481 tctctcttta tttatacgct cttgtaatac atctctatga gatgctacaa gtctattttt
     9541 tctaacatcc aattctttta tataaacttc taagttaaga ccaacaaatt tagatgtatc
     9601 tgtaataaat tttgtatcaa gttgagaaat tggtataaaa aatgtatatg tattgtaatt
     9661 agcaaataca cctttatcta tattcttttc aacatttaca gttattattc tatgttcatc
     9721 ataagccttt tgtaactcgg caaaatcatt tcttttgtca agttgtaatt tagataaagt
     9781 tattgtacca tctttttgag atacttttgt tattacagca gttatttcat cacctatgtg
     9841 gtatatatct tctatatatt gatttttttc tatgttcaat tcacttatag gtattattcc
     9901 atcaaaccca tagtctaatc ctaattgtac ttcatcattt ctcactgcag ttatttttcc
     9961 agttgtcaat tctccaactt tcactttgct gaatacttgt tcttgctggt caagtaattc
    10021 ttgcattgtt aaatcatttt ccattataaa catcctttcc tcttattgat tatcatatct
    10081 gtaaatatta taccattaat attgcaaata cttacatttt tcttactata atttttaaaa
    10141 taaattcaac ttcatatatt tatacattat agaaaatatc ttattaaatt aaaatacaaa
    10201 ttctaatttt tatctaataa aatattttca taaaaaattt atattgtata tcttattctt
    10261 ttattgcttt aggttttgat agaaaacatt tataaaatat aaagaaagaa attgctgtta
    10321 tagtagcagc tgttatatct gcaactggct ctgctaaaat tactgcaaaa agtttttcat
    10381 taaagaatgt tgggagtata aatattaaag gtattaaaag tacaatcttt ctaagtagag
    10441 ctaaaaccaa agatattgtt gcttttccaa gtgctaaaaa tgtctgttga caagctattt
    10501 gaatcccaaa cataaaagca ccagcaaaat atatttttat actccaacta gtcatactaa
    10561 gtaactgcgg attcttatta aatatactaa caaaaaatgc aggaaatatc attaataaaa
    10621 gccataaaat agtagaatat gtaaaacatg acaataaaca caacttaaat gtctttttta
    10681 ctctatcaag ttgacctgaa ccataattat aacttattat tggttgtgct ccttgtgtaa
    10741 cccccatatt aggaagcata agaatttgca ttatactgct cattatggcc attgaaccta
    10801 ctgctaaatc tcctccatac atttgtaatt tactatttaa ggctataagt actaaacttt
    10861 ctgtagattg cattataaat ggagagattc ccaagccaag cactggtaag atgatacttg
    10921 cttttggtat catatacttc ttttttattt taagtatact tttctttcca aagagaaaca
    10981 taagtaccca aatagcagaa acgccttgtg caattatagt agctaatgca gctcctttta
    11041 cacccatatt aaagccaaat ataaaaatcg ggtcaagtat tatgtttatt aaagcaccta
    11101 tagcaactgt catcattcca gttgtagcaa acccttgggt atttataaaa gtattcatac
    11161 ccattgaaat ctgaacaaac actgtaccta ttaaataaat atttaaatag tccattgaat
    11221 atctaattgt agattcactt gctccaaaca tccaaagtaa aggctctttt ccaatcttaa
    11281 aacctatagt caaaagtatt gctaaaatca ccagtacaga aaaactgtta ctcattattt
    11341 tctctgcctc atcattgttt tgctcaccca tttttatagc agctaatgga cctcctccca
    11401 taccgattaa ataactacat gcagttatta taattattat tggaaaggcc actccaacac
    11461 cagccatagc aacttctcca ttttccattc taccaataaa aatcctatcg actatgttgt
    11521 ataaaacatt tacaatttgt gcaataatag caggtgtagc aagtttaaat aataatttac
    11581 ccacactttc actgcctaag ttagtgtttg aattttccat tttaaaacct cctcaatcca
    11641 ataattaaaa tgtatcacta aaattttatt ctatataaaa atttcatttt atcagcagac
    11701 aaatttagag tactttccct acaagtaata ctatactata tttcttttgc catagtaaac
    11761 attttagaac taactgaatt taaatacttt atctcatttt ttatttatta ttaaaaattt
    11821 ttcacatgta ctcataaaaa aactatccaa gtatattact tagatagttt tatattatat
    11881 gtaaagaaag agctgaaact ataacccctg ataacaaatt gccaagaaat actcccataa
    11941 aagcatcttt tattctcatt tttaatatgg aagctatagc agatgcagtc catgttcctg
    12001 tagttggcag tggtatacct acaaatagta ttataccaat tatcgaaaca ctttttaatt
    12061 tcttcattct tgagtttatt tttctatcaa ttacatcagc aatcccttta aatagtttct
    12121 tccctcttaa aaattgtaat acatgtctga atgtcaatat caagggaatc gataccaatg
    12181 tagaacctat tacactaaat acataaaccc atataggatt taagcccatt gcaataccta
    12241 taggaatggc tcccctcaat tctgttacag gaaccataga taaaattaat atgtgtaaat
    12301 atcccaaact attccctcct attctaagtg acattaagtt atgaatttat ttgctctata
    12361 acctcaccat cctttttact aactaaaaat gcatttttag tcaatgaaag cattggtaaa
    12421 tcactcttag agtctgaata tgcatatgaa ttttcataat caatctctat cccaagttta
    12481 cctaatacag cctttattct ttttactttt tcaattccct tacagttttc tccaatgaat
    12541 tgatttttat atcttgaatt ttcataaaaa aactccgtgc ctataacctt gtctgcaaga
    12601 ccataatact taaaatattt catatatgca tatggagatg cagttatcat aattataaca
    12661 ttatcttctt ctttattttt gtaaatgacc tttttcatag attcgttaat ttttttcatt
    12721 atgcaatcat caaaaaactt ttttaaatct tcttcactaa aatctactat tggataaaag
    12781 attgcctctt ttgccttctt caagggtatc attctaatta aatacataaa tagaataaaa
    12841 ataatgtatg gcaactttat aatagtttta aaggttttat tttttaaaga gtaaataaaa
    12901 aaatctatca tgctatcagt atttataaca gtataatcta catcaagtaa taatacactt
    12961 tttttcataa gtatttcccc atcctatcac taaaaatatc actaaaaaat caatattatt
    13021 aaacatgtaa ttatccataa gattacgttt atttttaaag cattatcttt taggacaata
    13081 tcagtaggat tattttcatt tgtagtgtta attatataat tatatctaag tacaccataa
    13141 actacaaata ttgttgtaaa tatcatatgt ggcttttctg gattacttgt agaatacaat
    13201 gaataacata caattgtaga acttaatata actatcatca tttgatttag attttctatg
    13261 tcataatctt ccaatatttt tctatgttca actgcatcat ctcttagagt ttctatttct
    13321 tttttacgtt ttccaagagc taagtacaga gataagaaaa atgtacataa aattatccaa
    13381 tttgataaac taacacttat ggctacacat cctgcataaa ccctaagtat aaaaccaaga
    13441 gatattgaaa acacatctaa tagtattata ttctttagtt taagagaata taatatattg
    13501 tttatcaaat agagactaag aattatagaa agattaaaac taacaactgt tgataaacag
    13561 actgaaatta aagctaatac tacaaatagt atattagcac tttttttgct tattcttcca
    13621 cttgccactg gtctattaca ttttttagga tgttttttat ctttatccat gtctatagca
    13681 tcattcagtg catatactga tgatgacatt aggcaaaaac acacaaacgc aataaacgta
    13741 tttaaaaata aatttatatt tagtatgttg tttgaaaaaa ttatagctga aaatacaaag
    13801 aaattcttta tatattgctt tattctcatt aactttatat aatctgaaat tttagtaaga
    13861 agccttatac ttgtattggt ctgtttcata aacttactac cctttcaata ttatttttta
    13921 aacaggatat gattttatcc ctatgtttaa aatttctata aatccattga cattactgat
    13981 tactatagta accaataaca gcgatagaaa tatattatac atattctttt cattttttct
    14041 aagcgctttc gtaaagtaag ccaataaaat tataactgca aaactaaaat ttatagccat
    14101 aatacttgaa ttgactagat tatatcctac tataccatgt aaagttagat tgaatataat
    14161 tattcccata catagcttag gaactctttt ttctgaattt tcaataactg ataaaacaca
    14221 aaaacataaa gctaataatg taagaattac tggtattgta gcaacttctc cattttgcaa
    14281 aaataaccct tttttaatac ttgctccagg aaaataaaaa accgaataca ataatctctc
    14341 tataatcatt gcaatctttt ccaaaattgg ttctccactt ataaatttcg tatcatactg
    14401 tattacattt ccaaggttca taaaactctc ataagacttc gttgaaacta cacaaatata
    14461 tgccattagg ataaatgtaa ttaaatgttt taacattttt cctattttta atttaaactc
    14521 aaatatcata ttaaaaaaat acaaatatat attactaata gttattccaa atagaggaat
    14581 agctgctaat tctactaaat aagaattttt tcctttcatc ttatttgcaa taactatgaa
    14641 aaatattaat gaaaattggc tatatataaa tctctctaca tttaaagtca taaataactg
    14701 tggaaaagaa aatatcatta tcatagtaag taaaattaaa gttaatctct gcatttttat
    14761 ctttttaata tgattaaaga ctaaagttat tgacatagca ctagttatta tttgtagaca
    14821 aattagtcca aacccatagg cactattaaa atctgttgtc ccaggatttg ctattgaagt
    14881 acaaaatagg tatattggat atgcaaaaat tgatataatt gctgaaaaca atatgtgctt
    14941 actattgtct tgacttatag caaatacatt taaattgaat aaaacccctg tatctgtatc
    15001 taacaataca tcataagtat tgtttaaatt ataaaatata gtagtcttag aaaaaaatat
    15061 cttagttatt attaagtaag ttattacaac taatataaac aaaaacatct cgtttttata
    15121 ttttaatatt ttactcatca atatatcacc atccattcat tatataggat ttgctgacac
    15181 ctacaattta ttccttatat ttaatagcca tcttcataat gttcttactt gttttattgt
    15241 aattgaataa ctctttattt acaaccacaa tatttacttt attgatgtta taatcagtca
    15301 agtattgttt atataacata gattagtgtt gtataaaatg agaatttgta agcaatatta
    15361 atgtaaaata tttgaaaaac tttatataaa aatatacaat gttgcttatg gaattaaact
    15421 ataacaaatt aaagcataat ttaaggagac aaaattataa tctgtctcct taaatattaa
    15481 aactatattt taaaacatta ctaaatac
//
LOCUS       DAELXX010000095        15500 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.095, whole genome shotgun sequence.
ACCESSION   DAELXX010000095 DAELXX010000000
VERSION     DAELXX010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15500
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.095"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(101..835)
                     /locus_tag="KPH29_002811"
     CDS             complement(101..835)
                     /locus_tag="KPH29_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis protein TtcA"
                     /protein_id="HBG1468393.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMIYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
     gene            961..1677
                     /locus_tag="KPH29_002812"
     CDS             961..1677
                     /locus_tag="KPH29_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG1468394.1"
                     /translation="MKKFKLIGAIILVVFISVFISKDRLLKKDKDPIVAAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            1811..2503
                     /locus_tag="KPH29_002813"
     CDS             1811..2503
                     /locus_tag="KPH29_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1468395.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            2493..3674
                     /locus_tag="KPH29_002814"
     CDS             2493..3674
                     /locus_tag="KPH29_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBG1468396.1"
                     /translation="MKNKSLLNHMFRPILDLIISCALTSMLILTSMYIIKLLYFNSEI
                     SVINQLVYQLRDFRSLIGESLFYPFLFTITSLAIYSMISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKMEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            3956..4411
                     /locus_tag="KPH29_002815"
     CDS             3956..4411
                     /locus_tag="KPH29_002815"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBG1468397.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            4401..5126
                     /locus_tag="KPH29_002816"
     CDS             4401..5126
                     /locus_tag="KPH29_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HBG1468398.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIRINKDNVVELEGTNLTLESKDVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLNQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            5098..7479
                     /locus_tag="KPH29_002817"
     CDS             5098..7479
                     /locus_tag="KPH29_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HBG1468399.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDNAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSCGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(7684..8913)
                     /locus_tag="KPH29_002818"
     CDS             complement(7684..8913)
                     /locus_tag="KPH29_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBG1468400.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWSIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
     gene            9513..10427
                     /locus_tag="KPH29_002819"
     CDS             9513..10427
                     /locus_tag="KPH29_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468401.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGVKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
     gene            10451..11086
                     /locus_tag="KPH29_002820"
     CDS             10451..11086
                     /locus_tag="KPH29_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HBG1468402.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTAQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            11462..12178
                     /gene="pyrF"
                     /locus_tag="KPH29_002821"
     CDS             11462..12178
                     /gene="pyrF"
                     /locus_tag="KPH29_002821"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBG1468403.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVRAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGADFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            12204..13247
                     /gene="carA"
                     /locus_tag="KPH29_002822"
     CDS             12204..13247
                     /gene="carA"
                     /locus_tag="KPH29_002822"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBG1468404.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGEILTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIAPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            13300..>15500
                     /gene="carB"
                     /locus_tag="KPH29_002823"
     CDS             13300..>15500
                     /gene="carB"
                     /locus_tag="KPH29_002823"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HBG1468405.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYL"
BASE COUNT         6237 a         1714 c         2791 g         4758 t
ORIGIN      
        1 ataataacat ataataaata aaaaagtaag attgtgtaga ttatataaag attatttata
       61 attaatacaa tcttgctttt ttatttaaaa attttaatta ttatttaaaa ttttttatct
      121 tctctttatc ccatataaac aatttctcag aattatttaa agcgccaaat acatttcttt
      181 taaaatcagg catctcttta tttaaattag caataagatt ttttatgtac tctctcctag
      241 tatgaccatt tgcagggcag ggatttgtga taataggata gttagaatct tttgaaattt
      301 tcttagtcat atactcttct atgtaaatca taggtcttat aactgttaaa tcctgtctat
      361 ctaggtatgt ctttggggaa aaacaattta ctctaccctc ataaaacata gacattaaaa
      421 atgtctcaat tgcatcatct ttatgatgac caagagctac tttattacat ccaagttttt
      481 tagcattatc gttcaaagca cctcttctta aattagcaca taatgcacaa ggattttttt
      541 cttgtcttat atcaaataca atttctttta tgtcagtttg aaattcataa aaaggaacat
      601 ttatttcttt acacaattta tctagtggag aattatcaac acctccagta tttaatgtta
      661 tggctatcaa ttcaaagttt tgtggagaaa atctttgata acttttaagt atatgaagta
      721 gagttaaact atcttttcca ccagaaagtc caaccgcaat tttatcattt tcttgtatca
      781 tatcaaaatc ttgtatagcc ttacgagctt tacttagaag tttttgtata gacattttat
      841 caccatcctt taaaaatatt aaatttaaga tttatttaag atttacttat aaaatactta
      901 agtcttatac tctaaaatta acattataga caaacataac aaaaaggaga agatttaatt
      961 atgaaaaaat ttaaattaat aggagccatt attttagtag tatttataag tgtatttata
     1021 tctaaagata gattacttaa gaaagataaa gatccaatag tagctgctca acatgattca
     1081 gaacaagatg ttgaggcaaa ttctcaaaat aaaaatcact caaatagtat atcaaatata
     1141 ttattggtaa acaagacaaa tggaattagc aaaaactata ctcctgaaaa tattactaag
     1201 gtaaacatac cttttgttga agaagcaaca gaggaagaaa aacaaatggc aggagaacct
     1261 gcaaaggcag ttgaagattt agttaagcaa gctaattcag aaggaataca gtttttaggg
     1321 tcttcagcat acagatctta tgatactcaa ctggatactt atacaagaag ggtaaaatct
     1381 cagggacgag aaaaggcaga tgcatatgta gcaaagcctg gttacagtga gcaccaaact
     1441 ggactttgta ttgatttaac aaatccagaa agatggtttg ttggaagtac aaaagaagct
     1501 aaatggttgg cagaaaatgc tcacaagttt ggatttataa taagatatcc tgaaggtaaa
     1561 gaagatataa caggtacagc atatgaacca tggcatatta gatatgttgg taaggatgct
     1621 gccgaggaaa tttattcaaa aggattaact ttagaagaat atttacaaaa taaataatat
     1681 aaatgtgtaa ataatttgta ttatattgga aatttagaaa tgaataaatt tatagattac
     1741 actaactaaa atatagtata attttatatg aataaagcta tatacaaaga ttaagttagg
     1801 atgtgtaaaa atgaataata cagttcttgt tgtggatgat gaaaaggaaa taagagacct
     1861 tatagagata tatcttttaa atagtggata taatgtaatt aaagctgaag atggaaaaga
     1921 ggctctagat attttaaaaa aggaaaatat tcatttaatg atattggata taatgatgcc
     1981 taaaatggat gggatagaag tttgtagaaa agttagagaa agtttgaata taccaatact
     2041 tatgttaagt gcaaaatcag aagatatgga taaaatacaa gggataatga ctggtgcaga
     2101 tgattatttg acaaaaccat ttaacccact tgaacttact gtgagagtga aatcactgat
     2161 tagaagagca tattatttta gtggtgctaa taatgaagat aaagatgtca taaaggttgg
     2221 agtagtaatt atagataagg aaaaacatag tgtaacagtg gctggaaatg aaataatttt
     2281 gacatcaaga gaatttgata tattatattt actagcaaat aataaaggca gagtatatag
     2341 tacagaagaa atatttgaaa aggtatggaa agagaaatat taccaatcta ataatactgt
     2401 aatggttcat atgagcagaa taagggataa aattgaaaaa tatacagatg gagaaaaaat
     2461 aattcatact gtatggggag taggatataa aattgaaaaa taaaagttta ttaaatcata
     2521 tgtttcgacc catattagat cttataataa gctgtgcttt aacttcaatg cttattttaa
     2581 caagtatgta tataataaaa ttattatatt tcaatagtga gatatctgta attaatcaac
     2641 ttgtatatca gttaagagat ttcagaagtc ttataggaga gagtttattt tatccatttt
     2701 tatttacaat cacatcatta gcaatttatt ccatgatatc gtacaagaga aataagcaaa
     2761 ttgtagattt tataagaaag actaatagaa aaaatcaaaa attagaaatc attaaaagtg
     2821 ctgtaaattt aatggatgaa ggtaacttag aaaaaagtat agatattgag tatgatttag
     2881 atatattaaa ggataaagaa aaggatgata ttgatgaact agcacataaa atcaataata
     2941 tagtccatca acttagaaat ataactattg aagagagaca agctcagcag acaaaaacag
     3001 atttaataac aaatgtgtca catgacttaa gaacaccttt gacatccata atgggttatt
     3061 taggagtgat agaagaaggt aagtataaag atgaagttca gatgatgtat tatgttgata
     3121 ttgcatatga aaaatcgaaa aatttaaatg ttcttataaa tgatttgttc gaattgacta
     3181 aaatgcaaaa taatacaata aaactaaaca aaatagaaat aaatttagta gagttattaa
     3241 gtcaagtagt atctcaattt gaaatttatt ttaaacaaga atttatggtt agtagaatta
     3301 attttaatga agaaaaatta attgtgaaag ctgatccaaa taaattggta agagcatttg
     3361 aaaatcttat aactaatgct gtgaaatatg gaaaagatgg gcattatgta gatatagtca
     3421 cagagaaaat ggaagatatg gcggttgtaa aagtgataaa ttatggagaa ccaattccag
     3481 tgttagattt accaaatata tttgatagat tttatagagt tgaaaaatcg agaaatagaa
     3541 atgttggagg ttctggattg ggtcttgcga ttacaaaaaa tattgtaaat ttacatgatg
     3601 gagaaattac tgtttcaagc gataaatatc aaactgtgtt tgaggtacaa ctgccaataa
     3661 tacaatcagc ttagttataa aataaaagta cgattatgtg atataaatat tataacaata
     3721 aagtgtttac aaaaataaaa aagtgatata taatttaagt cagtttaata agatacttat
     3781 caagaattag gggagagagt tagctcagtg atcctatacc aaccttttat caggtggtcc
     3841 tgctaacaac gatgagatga cttgaaataa tatactcttc ttatcaataa tagataggaa
     3901 gagttttttt tagaattaat gtaacaaact ttaaagtata atggaggatt aaataatgga
     3961 aaaagtagaa agttttaagc tagaccatac aaaagtaaaa gctccttttg taagaaagtg
     4021 tagtgtactt gatggagtaa agggagataa agtaactaaa tttgacttga gatttttaca
     4081 gccaaatgta gaaagttttg gaactgctgc tatgcacggt ttagagcatc ttttagctac
     4141 ttatttaaga gatacattag atggtgtaat agacttatct ccaatgggat gtagaacagg
     4201 attttattta atactatggg gagatgtaga tgccaaaaca gttaagattg gtttagaaga
     4261 agcattaaaa aaagttcttg aatcagataa aatgccagca gctacagcca tagaatgtgg
     4321 aaactacaga gacttatctt tatttggggc aaaggaatat gcaaaagatg ttttagataa
     4381 aggattttct ttaaatatat atggagaata atttaattgg aagagaagat ataagcataa
     4441 atcaaagtga agttttaaga tatttacaat acagaggtca agagatagat aaagacctta
     4501 aatatactat tgatgaatgt gtagctataa ctaaaaataa aataaatcct agatatttat
     4561 ctaggattta tcctattaga ataaataaag ataatgttgt agaattagag ggaacaaatt
     4621 taacattaga gagtaaagat gtatatgaac ttttaaaaga atgtaatgag tgtgtaatta
     4681 tggcagctac tatagggata gatatagaaa aagaaataag acgacactct tattcaaatt
     4741 taacaaaagg actaataatt gattcatgtg gaacaacagc aatagaagaa gtgtgtgaca
     4801 ttgttcaaaa taatttagaa aaggtttttt taaaccaaga taaatttata actatgcgat
     4861 atagcccagg atatggagat ttaccaatag aaaaaaatat agatatatta aacgttttga
     4921 atgctcaaaa acaaataggt ctaacaatta cttctaatgg aattatgata ccgagaaagt
     4981 cagtagtggc tattataggt atttctaata caagaataaa taaatctaag aaagaaaagt
     5041 cgtgtgaaaa ttgtaaaaac tatagaaact gtacatatag aaaaggagcg aactcttgtg
     5101 gaaataagac aatatctgaa aaataatata ttaatatttg atggtgcaat gggaacaatg
     5161 cttcaacaaa aagggttaaa gttaggtgaa aatccagaag tatttggtct tcaaaatcca
     5221 gataaactta tagaaataca tacagcatac ttagaggcag gttcgaatgt aattcttaca
     5281 aatacttttg gatgtaatga actaaaatta gatagtaagt atactgtaga agaggtgatt
     5341 gacaatgctg ttttggtggc tagaaaagct atagaaaatg tagataatac taaaccaaga
     5401 tatgtagctt tagatattgg tcctatagga gaaatgctag agcctatggg tactttaagt
     5461 tttgataatg catatgagat atttaaaaga caagtactac aaggcgttaa atctggagtt
     5521 gatgtaatag taatagagac tatgatggat ttatatgaag ctaaagtagc tgtattagca
     5581 gcaaaagaaa actctgattt acctattttc tgtactatga catttgatga aggtggaaga
     5641 agttttactg gatgtatgcc agaatgtatg gtggctacta tagaaggtct aggtgtagat
     5701 gcaataggag ttaattgttc attaggacct aaacaacttt taccaatagt tgaaaaaata
     5761 gcatcaagag ctacagtccc tgttatggtt caagcaaatg caggtcttcc aaatatagtt
     5821 gatggagagg ctatatatga tgttgatgca aaagaatttt ttgaaggggt taaaaagttc
     5881 gttgaagttg gtgctactat aataggtgga tgttgtggta cgaatccaag ttttataaaa
     5941 gaaatatctg agaatataaa ttctgttaca aagggatgta tcgagaaaat tgataaatgt
     6001 gtagtctgtt caccatcaaa atttgtggaa gtacaatcgc ctacagttgt tggagaaaga
     6061 ttgaatccca caggtagaaa atctctacaa gaagcattaa aaaatgaaaa tgttgattat
     6121 gctataaatt taggcttaga acaagtaaat gctggagcac aaatacttgg agtaaatgtt
     6181 ggtcttccag agattgatga aaaaaaacta atgccaaaat taataagaga gattcaagca
     6241 gtagtagata cacctttaca agttgattct tccaatgtag aggccttaga gcaaggactt
     6301 agatattata atggtagaac aatagtaaac tctgtaaatg gcaaagagga gtctttagaa
     6361 agtatattac caatagttaa aaagtatggt tcatgtgtgg ttggacttac acttgatgaa
     6421 aaggggatac ctaaaaaggc tgaagaaaga tttgaaatag caaaaaggat agtcaataga
     6481 gcagtttctt gtgggataaa accaaaagat atatttatag actgtttatc attaactgtt
     6541 tcagcacaac aagaagaagc aatagaaact ataaaagcta taacaatggt aaagacacta
     6601 ggagtaaaaa ctatattagg tgtttcaaat atttcatttg gtcttccaaa taggaaagca
     6661 cttaatgctt cttttctaac attagcttta ggggcagggc ttgatttagc aattataaat
     6721 ccaaatgaat atagtatgat ggaagctatc aattctttta aaatattaaa taacacagat
     6781 aaaggatgta taaactatat aaatcaatat agtaatataa ataattcaaa aaaatcggat
     6841 tcatctacaa agattgacaa agatttgcca ttggatattt tagttgaaag aggattaaaa
     6901 gatgaagcaa aaaatgtaac actgaatctt ttaaaagaga aggatgaaaa ttacatatta
     6961 gatgagattt taattcctgc tcttgataaa gtaggaaaaa gatatgatag cggagatata
     7021 tttttaccac aattgattca atcagcagaa acggtaaaag tttcattaaa tacaataaag
     7081 gaaactttat taagtaaaag tagcaataat gtatccaaag gaaaaataat agttgctaca
     7141 gtaaaaggtg atatccatga tattgggaaa aatatagtaa aaataatgct agaaaattat
     7201 ggatatgagg ttatagattt aggtaaagat gtccctattg aagaagtagt ggaagttgct
     7261 aaaaagagaa atataaaatt agtaggatta agtgcactta tgactacgac tgttcaaagt
     7321 atgaaagata caattcaggc actgagagat aatgagataa atgcaaaggt atttgtagga
     7381 ggtgcagttt tgacagaaga atacgctgaa gaaatgggcg ctgattatta ttcaaaggat
     7441 gcaaagtctg ctgttgaaat agcaaagtta aatttttagc atataaatta aaataaaggt
     7501 tttactaaat aggatatttt atatataata taaatataag ttttagatac ttatgtttaa
     7561 aacaatagga attaatctat aataatgaga ttaattaata taaaaaatat ttatcatcaa
     7621 cattaaaaag ggctgggaag tctggtataa cttccattag ccctttttag ttaaaatcat
     7681 atgttaaaaa gcccaattac catttttaaa aatctgtatt tgttttccat cataagtctc
     7741 accaataata tccatatcag cagaaccaat cataaaatct acatgtgtta aactgtcatt
     7801 tacaccatgt tcatctaatt cttcatcttt taaattgttt ccaccttcca gacaagtctt
     7861 ataagcagaa ccaattgcaa agtggcaaga agcattttca tcatatagag tattataaaa
     7921 aattatatcc gtattggata ttggtgagtt gtatggaaca agagcaactt ctcctaaata
     7981 atgagagcct tcatccgttt caataagttt ttctagtgta tctaatccct tttcagcaga
     8041 gaaatctatt attttaccat ctttaaatgt taagaagaaa ttgtcaatta tgtttccacc
     8101 ataaacaaga ggttttgtac tataaacagt cccatttact ttaaacttat gtggcattcc
     8161 aaaaatctct tcagttggaa tattcggatt aaaacttatt ccattagggt ctttggatgc
     8221 tccacttagc caaacatgac cctttggcaa gtctagagtc aaatcagttt ttgaggattt
     8281 atattttaaa gttttaaagt tatttttatt tagaaagtcc attttagatt taagattttc
     8341 attatgttct tcccatgctt ttatagggtc ctgaccatct actcttgaac atttaaatat
     8401 agcatcccac agtttttcta ttgctttatc ttctgataaa tttggaaaca ctttttttgc
     8461 ccaatctttt gttggcacag agactatact ccatttacat ttatcagtta gagtgtaaga
     8521 acgccattct tttaaagctt ttccagatgc cttctgaaaa tttgcaactc tttcagggtc
     8581 aacatctttt aataaatctg gattttgggc atagactgat aaaaatgccc caccttcttt
     8641 tgctatatct acatattgtt ctgctgtcca tttggggaac tcatgaaatg cttcatctgg
     8701 agcatacaag tatttaataa gagtactttc ttcatcagac cactctatat aaacattttt
     8761 agcaccacat ttatatgctt ctttaagtgc atttcttgca aaatcagcac attctattgg
     8821 tgactttata agtagaattt gacctggctg tatgttaact ccaactttta ttgctaattc
     8881 agcgtatttt tctaaatttc tatttaaatt catatttatc ctcctatatt tttaatattg
     8941 aataacttat aaagtattat aacattactt atgtttttaa tattgaacaa cttataaaat
     9001 attataactt tacttagatt ttgggtcata ttcaaaatat ttatacttta tagattgaga
     9061 aataaaaaaa atattttaat taaaacagat tatgaggata acaaacttat ttaagcagta
     9121 tgttttttgt tgataataat attaaagtag aaataaaaat acttttttag tgaaaagcta
     9181 tacaagaata ccgaaaaaaa cttataatat aataatgaga tagatattta taaatgaaaa
     9241 aaatttttct ggcaaagctt aattaaaaag gtaatagcta gtgttgttat ttaaattagc
     9301 atagaaaatc aaataaaaaa attatgctaa tgtatagaca ttgataaggt agaatttaag
     9361 ccttttgaat gctatattaa actagagtag atattcttat gaatttcaaa atagggcagg
     9421 aggaaaaagg aaataaaact ataaaaaata taaagtattt atataaattt atagagttta
     9481 tacgtcagaa gatttcagtt aggagggcat aaatggagct ttatgaagcc attttttata
     9541 gaaaatctgt aagaaatttt tccagtaaga agataaaaaa gccattgatg gaagaaataa
     9601 aaagaagttg taaaaattta gaatacttaa atgaagattt aaatattaaa gcacatgttg
     9661 ttgatagagg tcatataata cattttttga tgggtaagga atgtaaagta aaggcacctc
     9721 attatatagt agttacatca gacaaaggtg aggactattt acagaatata ggatttgcta
     9781 cagaaggtgt agttttgcgc cttacaacat tgggaatagc tacttgctgg ttggaaggaa
     9841 acttaaagag agaagatatc ttggaatttg tagatattgg gaataagaat tttgaagaag
     9901 atgaggaaga tgatttagat tatctagctg gagtaaaaac agaagaagat gatgaaaaca
     9961 aaatagaaaa tccttatata ataatagcat ttggatatcc agcaaaaaat gaagagttgt
    10021 ttaggacgag atatgctaga cctgatagaa agccagtaaa agatatgata aataagataa
    10081 ataggaatag aaagtggaaa gatatcttag aattgacaag aagagctcca tctgtaaaaa
    10141 attctcaacc gtggtatttc cataaggatg aacgaggtct tcatttattt gaaaagagac
    10201 ctaagaaaca ctgtgaagat atgaataagg ttagcttagg tgtagcctta agacattttg
    10261 atatagcttg tataaaaaat aaaatagatg tttcatatga aaaacttcct ataaggaata
    10321 aaataggaaa atcatatttt ataactgtag tcgagcatgt gaaaccagaa gaagaaaccc
    10381 aagaagaaaa tgtaactcta gaaaaagaag aatcacaaga tgaataaaat tttggaaggg
    10441 gatattggat atgtataagt gtggaaaatg taatcttaga ggctgttcta atggaaatat
    10501 ggataaatta atgaagatat gtccaactag agaaattgaa ctacaacaaa aagcaaagca
    10561 actttatgaa gaagaagaga atagaaaaat agcttataat gctggattag ttgaagcaga
    10621 ggggtattgt caatatacta gactaaaaga gattatagag tttgctaaaa agtgtggtta
    10681 taaaaagatt ggcatagcat tttgtattgg actaaaaaga gaaatgcaaa cagcacaaaa
    10741 aatatttgaa catcatgggt ttgaagtatt ttctgttata tgtaaaaatg gggcaatgcc
    10801 taagagtaca attggactag aagcatctca aacaatatca aagtgttcag atgaggtaat
    10861 gtgcaatcca ataggacaag ctttattttt aaatgaagct aaggttgacc ttaatgtaat
    10921 tcttggatta tgtgttggtc acgatacttt agccatgaaa tatatggaag ctcctataac
    10981 aactttagtt gtcaaagata gagtaactgg gcataatcca gttgctgcaa tatataatgc
    11041 agaatcatat tttgagaaaa aattgtttga taaagatgaa gaataattca aagacttatt
    11101 gacataaaaa gcaattagtg gtttaattat aaaaaagata aaactttaaa ttatcctata
    11161 gatacataaa gttatataaa taaagaaaaa tattacagct aaattcctta aagtagtcca
    11221 gagagactaa agggttatag ataatgttag tatataacct ctagtaaatc ttctagaggt
    11281 ttttgatgag tataaaataa aaatttaaat ataataaagt cttttaaact agtccagaga
    11341 gactgggaaa gatatctcat agctaatgaa tggagttatg tatagataaa atccttatgc
    11401 ctcttttggt gtgaggattt ttttatgatt atttaggctt tattaaaaag gagatgtaaa
    11461 aatgataaat ggtaaagaaa aattgatagt tgcaattgat actgacgaat ttgataaagc
    11521 taaagaacta atagacagat tagaagatag tgtagacata tttaaggtag gtctagagca
    11581 atacgttgcc acaaaaggta aaacaataga ttatcttaaa gaaaaaggga aaaaaatatt
    11641 tttagacctt aaattccatg atataccaaa tactatgaaa agtgcagtga gagctgcagt
    11701 gagagataat gtttggttaa tgacaataca tgtatcagat atggaaggaa tgagacaatg
    11761 tgcactaatt gctagggaag aagcagaaag attaaatata gaaaagccat tagtagttgg
    11821 agtaacagtt ttaacttcat taagtaacca agatttacag gacataggat gtaatatgac
    11881 aacagaagag ttagctataa agagagctaa actggcaaaa gaatcaggca tagatggggt
    11941 tgtttgttca gcgcaagaag tagataaaat agtagaagca tgtggagctg attttataac
    12001 agtttgccca ggaataagac ctaaatcagc agaggttgga gaccaaaaaa gagttgtaac
    12061 tccatcagat gcaataaaaa aaggtgctca ttatttagtt gttggaagac ctataacaaa
    12121 agcagaaaat ccaagagaat cagctattaa tattgtaaga gaaatagaaa atgcttagat
    12181 aaaatatttg ggaggtagct tatatgaagg ggaaattaat tttagaagat ggaacagtat
    12241 ttgaaggaaa agcatttgga tatctaaagg aagctattgg agaagttgta tttaatactt
    12301 caatggctgg gtatggagaa atattgacag acgcaactta ttatggacaa atagttacta
    12361 tgacttatcc acttatagga aactatggaa taaatttaga agatgttgaa tcaaataagg
    12421 tgcaagtaaa aggacttatt gtaagagaaa aaagtgataa cccaaataat tttagatgtg
    12481 aaatggatat agacacatat ctaaagcaaa ataaggtaat aggacttgaa ggaatagata
    12541 caagagcatt aactaaaatt attagaaaca atggaactaa gagaggaatt ataactttag
    12601 aaaatgtatc attacaagat gttcagtcta aattagaaga gcattgtaac aaagattcag
    12661 taaaaaatgt tacaagaaaa gaaattgaaa atataaaagg tactggagct aaagttgtag
    12721 ttatagactt tggtgttaaa aatagtgtac tagagtctct taaagcttgt aactgtgata
    12781 taactatagc accagcaatg actacagtag aagaaatttt aaatataaat cctgacttaa
    12841 tcttattatc taatggacca ggaaatccag aggatataaa tgatatagtt gaaaatataa
    12901 aaaaattaat aggcaaaaag ccattgacag gaataggttt aggacatcaa attttagcat
    12961 tggcattagg aggaaagact tcaaagcttg tatttggaca tagaggaggt aatcacccag
    13021 ttaaatgttt agaaactgga aaggtattta taacttctca aaatcatgga tataatgttt
    13081 cagaaatgcc agaaaataca gaggtaactc acataaattt aaatgacaat actatagaag
    13141 gaatgagaca taaggaatta ccaatatata gtgttcagta cattccagaa tacacaacaa
    13201 aagaagattt agattgtata tttaataaat tcttagcatt aatatagatt tagtattatt
    13261 tacagatagt aaaaaccatt tagataagga gaaaaattta tgcctaaatt agatagtata
    13321 aagaaaactt tagtattagg gtcaggacca ataataatag gtcaagctgc agagtttgac
    13381 tattcaggaa cacaagcttg ccagtctttg aaagaggaag ggattgaagt tgtattaata
    13441 aatagtaatc cagccactat aatgactgat aaagaagtgg cagataaaat atatatagaa
    13501 ccattaacaa tagaatttat agaaaaaata atagaaaaag aaagaccaga cagtttactt
    13561 gcaggtatgg gtggacaaac agggctaaac ctagcagttg aactttatga aaaaggcata
    13621 ctagataaat atggagtaaa gataataggt acttcagtag aatctatcaa aaaaggtgaa
    13681 gatagagata tatttagaga agtaatgaaa gaaataaatc agcctgtaat agttagtgat
    13741 atagttacag atttacaagc aggtcttgat tatgcactta caattggtta tccagtagtt
    13801 gtaagaccag catacacact aggtggaact ggtggtggta tagctgataa tgaggaagaa
    13861 ttaagagaaa tactttcaca tggtctacaa ttaagtcctg ttggacaagt attaattgaa
    13921 aagagtataa aaggttggaa agaaatagaa tatgaggtaa taagagattc aaagggaaat
    13981 tgtatagttg tatgtaacat ggaaaatata gacccagtcg gagttcatac aggagacagt
    14041 atagttgttg ctccaactca aactttaagt aacaaagaat gtgcaatgct taaaaaagca
    14101 tctttagata tattaaatgc agtagaagtt caaggtggtt gtaacgttca atttgcactt
    14161 aatccacata gttttgaata tgcagttata gagataaatc caagagttag tagatcatca
    14221 gctttagcat caaaagctac aggatatcca atagctaaag tttcatcaaa aatagcttta
    14281 ggttatactt tagatgagat agaaaatgca gtaactaaaa aaacttatgc ttgttttgaa
    14341 ccaactatag attatgtggt ggcaaaaata ccaaaatggc cttttgataa atttaaaaaa
    14401 gcaaatagaa aattaggaac taagatgatg gcaactggtg aaattatgag tataggaagt
    14461 aactttgagg ctgctattct taaagggata agatcacttg aaacaggaaa atattcatta
    14521 gttcacacac catctgagga gagaagcatt gaggaattaa agaaaagagt tgtagtacct
    14581 gatgatgaga gactatttga cttagctgaa atgataagaa gaggctataa agtagagatg
    14641 atagaacaaa ttacaggtat agataaatgg tttataaata aatttaaatg gatagtagag
    14701 caagaagaaa aattaaaagt tatgaaaatt gaagatttaa ctaaagacta tcttcttgaa
    14761 ttaaagaaaa agggattctc tgataaaggt atatctgact taatgaagat aagtccagaa
    14821 aaattatatg aattgagaag tctatacaat atcaaaccag catataaaat ggttgatact
    14881 tgtgcaggtg agatagatgc tatatctcct tactattatt caacttatga gcaatatgat
    14941 gaggtagttg ttagtgataa gaaaaaggtg atagtattag gttctggtcc aataagaata
    15001 ggtcaaggta tagaatttga ctattgttca gttcattgtg taaaatcttt aagaaaaatg
    15061 gacatagaaa ctataatcat aaacaataac cctgaaactg taagtacaga ctttgataca
    15121 tcagataagc tgtattttga accattaaca gaagaagaag ttttaagtat aatagaaaaa
    15181 gaaaagccag aaggtgtaat actacagttt ggtgggcaaa cagctataaa attggctaag
    15241 tttttacatg aaaaaaatat acctatactg ggaacagatt ttgatgatat agatgcagca
    15301 gaagatagag aaaaatttga tgatttacta gagagactag atataaatag accaaaagga
    15361 aagggtgtat ggtcacttaa cgaaggtatt gaagttgcta atgaattagg ttatccagta
    15421 ttagttagac catcttatgt acttggtggt caaggtatgg aaataactta taacgaagaa
    15481 aaattatctc aatatctaca
//
LOCUS       DAELXX010000096        15144 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.096, whole genome shotgun sequence.
ACCESSION   DAELXX010000096 DAELXX010000000
VERSION     DAELXX010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15144
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.096"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(556..1149)
                     /locus_tag="KPH29_002824"
     CDS             complement(556..1149)
                     /locus_tag="KPH29_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG1468406.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
     gene            1710..1940
                     /locus_tag="KPH29_002825"
     CDS             1710..1940
                     /locus_tag="KPH29_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468407.1"
                     /translation="MRLDMDSMLYVLIYMTLLVSIIGSIFYFCKDLLNHKIKYVLIRD
                     IFVNRITYSEERLPKQYIVFQKYDIWRYCSLF"
     gene            1997..2647
                     /locus_tag="KPH29_002826"
     CDS             1997..2647
                     /locus_tag="KPH29_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBG1468408.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEHIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRMYFNRKNRIVTK"
     gene            2861..3532
                     /locus_tag="KPH29_002827"
     CDS             2861..3532
                     /locus_tag="KPH29_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1468409.1"
                     /translation="MAKILVVEDEKRMQSIIVEYMQKGGYTCITANDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            3529..4659
                     /locus_tag="KPH29_002828"
     CDS             3529..4659
                     /locus_tag="KPH29_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1468410.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKFEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDAISHEMKTPLGIIRAYTEGLMDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLIINAKKHVVYNGNLDLSITCENGLLLFEIYNDGRIIEQDEISKIWSKFYRSVQSQ
                     RKGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            5064..5909
                     /locus_tag="KPH29_002829"
     CDS             5064..5909
                     /locus_tag="KPH29_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1468411.1"
                     /translation="MIKVGELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSLFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMVIDKPKNL
                     KMKNSNNKVEVEFVYNGNRDIEIVDIQELESGITFKYDEILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            5909..6937
                     /locus_tag="KPH29_002830"
     CDS             5909..6937
                     /locus_tag="KPH29_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1468412.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDNYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFLICDLCFTVLLVV
                     FNIGFFEGVKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            6940..7953
                     /locus_tag="KPH29_002831"
     CDS             6940..7953
                     /locus_tag="KPH29_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1468413.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTML
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGAPLLIYLLGVN
                     TSGIGNLCYIMPSVATFNGIMEIINGNVIIIKDIVILVVHCVVWFMIYIILDKKRKLS
                     FSR"
     gene            complement(8435..9685)
                     /gene="cme"
                     /locus_tag="KPH29_002832"
     CDS             complement(8435..9685)
                     /gene="cme"
                     /locus_tag="KPH29_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HBG1468414.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHNIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLVMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVISINNLIIVAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(9771..10517)
                     /locus_tag="KPH29_002833"
     CDS             complement(9771..10517)
                     /locus_tag="KPH29_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG1468415.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNQYLQQLEIEKSN
                     AEEALTIVAQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSNPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            complement(10632..11654)
                     /locus_tag="KPH29_002834"
     CDS             complement(10632..11654)
                     /locus_tag="KPH29_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBG1468416.1"
                     /translation="MNKNNCLDLIFEKVDPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKVVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTCSPSEICKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSIGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWICKKDGVLWYSAMGTGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            complement(11654..14074)
                     /locus_tag="KPH29_002835"
     CDS             complement(11654..14074)
                     /locus_tag="KPH29_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG1468417.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLALLYSVEGQIMCTMCDTPVSDNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEIRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKILINQGNLNTFGTNLVNDILSKTRHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSIKKLKDLGNTVI
                     VVEHDKNTIEMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSDKSLTGQYLSGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQINSSDSEIGDEDDYAVIETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLERGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECQGKRFNDESLSIKYKNKNIHDILNMSVSESVHFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTSK
                     LIQLLDELVENGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKTLKENP
                     KSITGRYL"
     gene            complement(14160..15080)
                     /locus_tag="KPH29_002836"
     CDS             complement(14160..15080)
                     /locus_tag="KPH29_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG1468418.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPHNHPQNRHFVDIYLPIEPF"
BASE COUNT         5330 a         1986 c         2362 g         5466 t
ORIGIN      
        1 taatttaaga aatatagaaa attaatttaa agaaatacaa tacattaatt tttaagtaac
       61 attaaattaa tgtaagtaaa ataatgttca tatgaagatt taaaggtgca ttttctgaag
      121 ctaagtatat gttatgtctc tatatccggt gaagtttaat agctcaagaa aatgcactat
      181 ttatgatgat tattttgttt tatttccaat tttggaaata actattctaa ttaaatctaa
      241 aattatacca taaataagaa aagaaagtat agatgctatg taccaataaa tcgaaattga
      301 ccctgtaatt gttctacatg ggatttgcat gcctgaatcc ataaaaacaa aaaaactatc
      361 aatggtagaa ataatagaca aaataggatt taatcctatt agaataatac tttttgagtc
      421 ttgaccaatt tggtggatat aaatctcaaa taatgcaatt aaaaaaaaca aaatgtaaaa
      481 gatttaataa tttttttcat caaaatacct cacctaatat aaattctgtt tgcactattt
      541 gcttactata actatttatc tccattcagt cacatcactt aattgtaatc taggtcttct
      601 tgatggttct tcgtcaggat aacctaaaga tattgctgcc actaattgac tatctgtatt
      661 taaccattca cataattcac gatatgcaaa gtatacatca caaatccaaa gactacctag
      721 acctaattcc acagctgtaa gcgacatatt ctgaattgca gctccaattg attgcatgtt
      781 tgccatttca tagaatcgtt cttcagggga tagcttttct aaaggtgatt tacctaatat
      841 atttaaaata aaaattgtta ctggagcttg tttcatgatt tcaactgtgt aatttgcacc
      901 agcgatatgt tgacgacttc ctggaagaag cccattatca ttaatttcat tttgaatacc
      961 tttgctcatg gctttaagca tagattcttt ttccttttca gtgatgacaa caaaacgcca
     1021 tggttgcctg tttttagatg aaggtgcatt tattccagct tcaattattt tctctatagt
     1081 ttcatgtgaa atgctttgat ttttatattt tctgatactt cgtctttttg atatagaatc
     1141 acttatcatt ttaagtaacc tccgtttatt ttttggaaaa atctatttat tttctatttt
     1201 taattatttt agcattaatc agaaaaaaag tcattgattt tagtatacaa ttattaacga
     1261 ttatattaag gattgtatca aaaagtatgt gattatacta agggattata ttagacatta
     1321 tatttaaaat tgtatgaaag gttgtattta atattatatt aagagagata tatattagga
     1381 tttatatata ttaagattta ttatattgag cccttatatt aaattaagga ttataattta
     1441 aaaataagct ataaaaaccc aatattgttt tgttcagaga taaaaaagaa tactttaggt
     1501 gatttttatt aaaaattaag agtgataaga ttatatactt tataaatgta aatatgacga
     1561 ataaaaatga taaatataat aatctatata tttacattag gagttttcta tactataatt
     1621 atttatattt aaaactaaat ataaatgttg gaattattta ggatttgaaa ttgtctaaag
     1681 ataaatataa gtgatgaaga ggtgatgtga tgagattaga tatggatagt atgttatatg
     1741 tactaattta tatgacatta ttggtatcta tcattggaag tatattttat ttttgtaaag
     1801 acctcttaaa tcataaaatt aagtatgttt taataagaga catatttgta aatagaatta
     1861 catattctga agaacggttg cctaaacagt atatagtttt tcagaaatat gatatttgga
     1921 ggtattgtag tttgttttaa tttgaaatga atctattata aattctaaag tttaaaaata
     1981 aaaaggagag aataaaatgg atttcatttc aagtatattg caaggattat taagtatatt
     2041 atttaatttt acaggtgatt ttggaatttc tattgtactt ataactttaa tagttaaatt
     2101 agtacttatg ccactatcat taaaacaaag attttctgta aaaaaacagc aagaattagc
     2161 tgaaaaaatg gaacatataa aagaaaagta taagaatgat actaaagaat tggaaaagca
     2221 aatacaagta agttctgtag aaagtatgaa gagtatgctg ggatgtctta tagttttggt
     2281 tcaaatgcca gtagtatatg ctttatatca tgtatgttta gatatgacaa aggaatttac
     2341 aacaattata attccatgga ttgctaattt aggtacattt gataatttgt ttatattacc
     2401 ttgtatatat acattaatta tgttagcttc aaatttagtg aattatatac catatttaaa
     2461 aataaattct caagtaaagt ttaacaaaca aatggctgtg gtgactacca taacaagtct
     2521 tatactaact gttagaactc cagtagccat aggtctgtac tttataacaa gtgcaattta
     2581 ttcattaatt gaagatattt gttttagaat gtattttaat cgaaaaaata gaatagtgac
     2641 aaaataatat aattattatt agaaatattt tagcctgcta acatagaaaa agttagtagg
     2701 ctatttttat ctatgatatc aattccatta agaatagtct aaaaaatact atcaaaatgc
     2761 tataaaaaat actataaaaa tactataaaa aaatagttga aaaataaata aattaatata
     2821 aactaaaata aagcctacaa aaaagaggtg ataaatagaa atggctaaaa tactagttgt
     2881 agaagatgaa aaaaggatgc aaagtattat tgtagaatat atgcaaaagg gaggatatac
     2941 ttgtataact gctaatgatg gagtcgaggc acttacaata ttaaagagca acaatataga
     3001 tttaatgata ttggatatta tgatgccgta tttagatgga ttttcagtgt gtagagtttc
     3061 acgtgaaatg actaatatac ctattatcat attgacagct aaaggtgaag aggaggacaa
     3121 attaaagggt tatgaatatg gcgcagatga ttatataaca aaaccattta gcccaaaagt
     3181 acttttagca aaagttaatg ccttgttacg tagatacact actgatattc ccaaaaattc
     3241 attgagttta ggaaagattt ttattatggt agcatcaagg caagtgtatg tagaagataa
     3301 attgattgat ttaacatata aagaatttga attattgcgt ttatttatgg aaaaccctaa
     3361 tcaagttttt tccagagaga agcttttaaa ttgtatatgg ggatatgatt ttgaaggaaa
     3421 cacacgaact gtagatacac atataaaaac cttacgcaaa aagcttggta gtgaaggaca
     3481 ccatatagtt acattaattc gttcaggata taagtttgag gttaaggaat gaatagttta
     3541 agaaagatta tagaaaaact gaaaaatgta gatatttcaa attggattaa acaaaatttt
     3601 ggactatatt cagttagaaa aaaagtgttt ttactttcta aattatcagg agcgttaatt
     3661 atattatttt atctggttgt tgaagaattt tcgataaact ctaaatttga attttggatt
     3721 tggtttataa tactggttat atttattatg agtattgatt ttttattggg aaaatttatt
     3781 tcagaaccaa tcacatctat aaataaatct gcaaaaagta tgtcccaact tgatttttct
     3841 aatccatgta ctgtaaatac taatgatgaa tttggagaac tttctaggag cctgaataca
     3901 atgtctacaa atctacaaca ggcattatca gacctagaaa gtgcaaatat tcaacttgaa
     3961 aaggatgtaa ataaggaaag aatgctattg gaacaaagaa aggagttggt agatgcaatt
     4021 tcacatgaaa tgaagactcc acttggcatt atacgtgcgt atacagaggg gcttatggat
     4081 gaagttgatg aagaaaaaaa gaaaaattat atgaatgtta taatagaaga aactgataga
     4141 atgaacaata tgattgtatc attattagac ttatctgcat tggaagcagg agtttctaaa
     4201 cttaacccag agcgttttga ttttatagag tttgttgaaa ctgtggcagg tcgtctttta
     4261 attgatatac cagatgccaa tttttatttt acatatgatt tgccagagta tgaagttttt
     4321 gtagtggcag ataaaatgag aatggaacaa gtagtagaga atctaattat taatgcaaag
     4381 aagcatgttg tatataatgg taatttagat ttatcgatta catgtgaaaa tggcttgctt
     4441 ttatttgaaa tttataatga tggtaggatt atagagcaag atgagatttc aaagatttgg
     4501 tctaagtttt atcgtagtgt tcaatctcag cgtaaaggag gttcaggtct agggcttgca
     4561 attgtttcac aaatacttac aatgcaaggg ttagaatatg gagtgaataa tcgtgataga
     4621 ggagtagagt tttattttat gattccaatt gatgaataat ttagtattaa tttataaatt
     4681 ataagtatct gatgtaaaat atttttcttt gtatacgtaa gtatttaaag agaataaaat
     4741 aaaaaactta atgaaaaagt ctaataaaaa gtttaatgaa aagtcttgtt ttcctgatga
     4801 aaacaagatt ttttattaag ctttttatta tttcacttcg atttcacaag agagacactc
     4861 tgatttcacc aaatagaaat aaaataggtt aaataaatta aaaaatagaa gcgtaattaa
     4921 ataaggttac aagggggagt acttatgaaa tggtatacat ggttgactat tatattgata
     4981 atattgcttt ctattccgct taaaattaaa tttataaaat ggtgggatag acgacaaaaa
     5041 aatgataaag gaggttatga tagatgataa aagtaggaga gttaaaattt tcttatggaa
     5101 aagataaaca gatattgcat ggacttaatt ttgaagtaaa agaaggagaa atctttggat
     5161 tccttggacc taatggttca ggaaaatcca caacacagaa aatcttaaat ggcgttttaa
     5221 aaggttacaa tgggcacgta tccctttttg ggaaggaagt taaagcatat acagagtctc
     5281 tatatcaaaa gattggtgtt ttatttgaat ttccttattt atataccaat ttaagtgcaa
     5341 ttgataattt agaatatttt tcgtcttttt atcctaaaaa tcaaaggaga gacatatatg
     5401 aactcttaga tttgttagag tttaagaaag agtttataaa taagcctgta tcatcctact
     5461 caaaaggtat gaagcaacgt attagcatgg caagagcatt ggtaagtaat ccaaagttgt
     5521 tatttttaga tgagcctact agtggacttg acccatctgg agctgtacta tttcgtaaga
     5581 ttattgaaga agaacgaaaa aaaggaacaa ctatcttttt gactactcat aatatgctag
     5641 atgcagattt aatgtgtaac aatgtggctt ttattgcaga tggcaagatt atggtaatag
     5701 ataaacccaa aaatttaaaa atgaaaaata gcaataataa agttgaagtg gaatttgtat
     5761 ataatggaaa tcgagatatt gaaattgtag atatacaaga gttagaatct ggaataactt
     5821 ttaaatatga tgaaatctta agtgtacatt caaaagaacc tacactagaa gaagttttta
     5881 ttaaatacac aggaaggatg ttagtgtaat gtattctaaa ttttttacct tagtaaaaaa
     5941 agatttttgg atgatgattt atggtaaatt ttttgtgctg acacttggct ctctcatact
     6001 ttattcgtgt tatattaatt ttggttatgt aaagtttatg gatgaagata gttatcacaa
     6061 taatgtatat ctatataatc cagagaatat aagcatgagt gcatctccat taattaatca
     6121 agtttcttct ataggtgaat taaaagcaaa acttgatgaa gatacaagtg cagtttgtat
     6181 tgatatgagt tccagtaagc cgaaagtaat tttacatgaa agtattaaag gagttgacaa
     6241 ttatagagct gactatgcat tgtcattatt atcatcagaa aaagaccata atagcaaaat
     6301 atcagacaac aatgcaataa ttcaaattaa tgataaggat acatctgaga taaaacaaag
     6361 aaaagacatg acttgtgaga ttttattctt tgaaattgta gctattggat ttttagggat
     6421 tgcctcttta ttatttaaag aaaagcagat gggagtaatg cgtattactg gtattttacc
     6481 tgtaaaaaga agtcttttta tattatcaaa gatatttgct tttttgatat gtgacctatg
     6541 ttttacagtt cttttagtag tatttaatat tggatttttt gaaggagtaa aaatacttcc
     6601 acaagtatta gttcaagcta gtattttatc aattattatg tctttgattg gaataggatg
     6661 ctcaatgtta ttgagagatt ttaagcaatt ttctcttata tatcttgtgg ttgctgtatt
     6721 cataactaca cctatatttc tatctgcaaa tacatctttt gaaatagatt ggattaaata
     6781 tcatcccttc tatcatgttt atagtcagct taaaaatgcg tttttcatga atttttcaaa
     6841 agaattagtt tattttgtag tgtgttcttt gacaataata gttctatttt tacttgtaaa
     6901 atatattttt aataaagagt tggttaggga gggataatct tgataggatt acgttaccag
     6961 ttaaagagta taaaaaaaga taaaatgtgt ataatttcct tctttttacc agttgttatg
     7021 gcgattttaa taaattttat gggaacaatt gatatatctt cattaggaga gatacgtttt
     7081 ggtatagtga aggatacact tcctgtacaa acagagcagt ggttgaagaa gtatggtatg
     7141 gtaagtgtat atgacacaaa aatgcattta atacgtgaaa ttaaggatat taaaacggac
     7201 acaattggag ttgttagaga tggtaaagga ataaaaacta tgctttatgg aaatgagatt
     7261 aatgctataa aaaatgttgg aaagacattg ccagagatgt ttagactaaa gggtgattta
     7321 aaagatgttg aggttacaat attgcctaaa gatagtattt taaaagagtt tcagaatgtt
     7381 tttattgcta tgattttaat tactgctatg tttatgggat gtacctttaa tgcaatgaat
     7441 attatatctg aaaaagaaga tggaatatca tttataaatg aagttttgcc acagagtaga
     7501 agtcagtata ttatacaaaa agtatttttg ggtcttttat gtgggtgttt atcttcaatg
     7561 ataactgtat ttatatgttt ggatatttca atattagata tgataatgat gttgttactc
     7621 gttgtatttt cagcatttat ttcatcactt ataggtttat ttattggaaa gttgtcagaa
     7681 ggtcttatgg taggaattgt ttatattaaa atagtaatga ttttatttat aggtgctcct
     7741 ttgttaatat atcttctagg ggttaatacc agcggtatag ggaatttatg ctatataatg
     7801 ccatcagttg ctacatttaa tggtattatg gagattatta atggtaatgt tattattata
     7861 aaagatattg taattttggt tgtacattgt gtagtatggt ttatgatata tattatttta
     7921 gataaaaaaa gaaagttatc ttttagtaga taactcagta taattataat tgggacaatt
     7981 taataggtga tttaatatac tttgtatagt gtaatttatg tagtgcaatt tgtatacttt
     8041 aatttatgta gtgtaatagt tagttttatt ttataattat tcaatataat ttagtaagaa
     8101 attcagtaca attagagcta cttagtggaa aacttaatag gtacttaaac tatataaata
     8161 ctttattttc ctccatttgt actataaatt taatttatga tataaatgga ggtattatta
     8221 tatgaggtat tattatataa agttaataaa gatagtatca aaatattatt tgctataaag
     8281 agagggactc aaaataaaga taaacctatt tttcaatgtt gaaatgataa aatatatttg
     8341 atttcatata aaaaattttc ttcataaatt aaaaagtcca ttttaagccc cctcattttt
     8401 aaccagataa atcgagttaa actatttaaa tatattactc ggattcattt gacttagata
     8461 ttccttgctt gtaaaattgt tttgaatagg ggatagccaa agcaaaaaat aaaagtataa
     8521 tccctgctac gattattaaa ttattaatag aaattacatc tgccattggt ccgaaaatca
     8581 tcataccaag aggaataaaa ccagtatata taataatagg taagctaaga actcgaccta
     8641 gcatttgtgg tgctacatgc tcttgcaaca tagtcatagc tgatgcttga ataattggaa
     8701 ttgaaaaatt cgtaaaaaac ataaatataa aaaattgcca agcttgtgtt acaagaccta
     8761 gaataattga aaaaatagca taggataata ttgctagaaa aaaggtctta tttctatttt
     8821 taaaaccacc aaaaactcct ataatcaatc cacctaaaac cataactaaa aatccaatca
     8881 tttcaataat agacaggtat atataaccct tgccaaatgt tcgttcaata agaagtccta
     8941 taagaaatcc tgatggaaca caaagaagca tgaatgctcc ataagtgaaa agcacttttc
     9001 taatgaactt atctgaaaaa cagtacttta tccctgatac catatctgca aaaaatgaga
     9061 ctttttcagt ttgttttggt atattatgtt taggaatttt gatgaatatc aataatacaa
     9121 tagcaataat tgcagtaaat acatcaatta aaagaacttg atttatttta ttaccaaatc
     9181 caagtattgc agcagctaca ataggcacta taaattgtac tatagactgt agactactat
     9241 ttataccatt aaaacgcatt aaatgttctt ctggtacaag ttgaggaatc attgcgttta
     9301 cagcaggtgt ttgtacacca cttcctagtg aacgtataac agatattact gatatggcta
     9361 ttaattcact tgttgtgcca tttgatgaca tcataaatag aattagaact aatgtagata
     9421 tagcaattgt tgaatctgat acaattatga gtacctttcg attgtagtag tctgcccata
     9481 caccagcaaa aaaagaaatt atcatttgag gtataaagga gcatacagtt aaaactgtta
     9541 cccatacacc tgatgatgtc tgaagtgtga cataccatat cattgccatt tgtacaagtg
     9601 atgagccaaa taaggaaata tattgacttg ctaaaaaaag aatgactttt ttcttccatg
     9661 tagaattagt atttttgttt tccatcctat tcctcctttg atttgtgtgt tgaatttttc
     9721 ttgctcttaa ggataacaca caaatcaaag tctggagtta gagtggaggg ctaggaaaat
     9781 tgttttttca taatttgaag atgagatttc atggtttttg cattatgttc agcttctttc
     9841 aatgaagtta tcagtttgtc acacactgag ataatttcgt catcatcttt tggagtgtca
     9901 aggacagttt taatattagt attaggatta gaagataaag catttaacat tcgtaaaata
     9961 gctgaaagag aatagtttgc acaacgaagt gcgcttataa ttttaagtcg ttttatatct
    10021 tcatcagtat atacacgata accattttgt tttcttttta cttttaacaa accattcatt
    10081 tcccaatttc ttaaagtatc tattgtaaca tttaagtgtt ctgcagtttc ttttctagtt
    10141 aaaaagagtt tatttgtttc tggagtattt ccatttaaaa tctgtgcaac aatagtaagt
    10201 gcttcctcgg cattagattt ttctatttct aattgttgca agtattgatt tacaagacta
    10261 atagccttat caaagtccct attcgctgat gttcttacaa tctcaagggc atcttttctt
    10321 aatccccttt gtaccacttc aattttcaag gctgtacgag caaatctaaa ttgttctata
    10381 tgaaaatcat taaagatacg ataaccattg gtattacgct taggtttagg tattaagttt
    10441 agttcttcat ataaacgtac tgtattagga tgtacaccaa ttaaatctgc tacctctgat
    10501 gtcgtgtaag tgtccattag tattcaactc cttttagata tatagtatta gtcataataa
    10561 caccaaacaa aagtctggtg ttatagtgga gggtttggtt atctaagagt aattagttta
    10621 tctaagagtg attaatctat tatagaagta agtatatatt tttcaataaa ctcccatcta
    10681 tcacgaggtt taaacactat tttagatggt atggctatta ctaagtcata ttgtggaata
    10741 caacaaatag catttcctcc agttcccatt gctgaatacc acaaaacacc atctttttta
    10801 catatccacc acagataacc gtattcatta ctattcattt taaccgattc ttcaatccac
    10861 gatttagata taatacgttt attattccaa ataccatcat ttaaatacag aaaaccaaat
    10921 cgcaacatat ctctagcact caatgtaagt ccccagccac ctatagaata accctgtcta
    10981 tcttttgccc aacccttaag attttttcca aagaaatctt caaaaccaaa atctttcata
    11041 tcataatctg gtataacttt cataccaata ggtttaaata agtactcatt agcaaattca
    11101 cgagcagatt ttccagtagt ttttgttata atagctgaaa gtaggtgcgt tccagctgaa
    11161 gaatatttga aagtaccaat ttcgcctttt tgaccaagtt gattcaaggt atattttatc
    11221 caatcttttt gtttgcaaag tctttctaaa gactcagtca tatttttaaa agggtatggt
    11281 gcagtcattg taagtaggtg acgtatagtt actgcttttt tacatatttc agaaggacta
    11341 caagtatatt cagggaaaaa atcaacaacc ttttggtcaa catcttttat atatccttta
    11401 tctattgcaa tacctattaa ggctgatata atactttttg taactgatgc aacatgaaaa
    11461 gtatcatcag cattatatcc attatagtac ttttcaaaga caacttttcc ttttcttgca
    11521 ataattattc catttatatt actatattgt gagattatta aaggttccag ttgtgagagc
    11581 ttttctgaac tcatgccaac acttgaaggg tctacttttt caaatattaa atctaagcaa
    11641 ttgtttttat tcattaaaga tacctccctg ttatagattt tggattttct tttagagttt
    11701 ttggagaacc ttctgcaatt atataacctc ctttgtcacc accttctgga ccaagctcaa
    11761 caatccagtc acaaacattt aatacatcta tatcatgttc aacaacaatt acagaatttc
    11821 cattttccac caattcatct aataattgaa tcaacttaga agtatcatat agacttagac
    11881 cagtagttgg ctcatccaat acataaagga tattaccatt tcgtttttta ccaatttctt
    11941 ttgcaagttt gattctttgg gattcaccac cacttaaagt cggtgtaggt tggcctaatt
    12001 ttatataccc catacctatt tgttctaata catttaagtg tgaaacaata tttggaatat
    12061 ccttgaagaa atgtacagat tcagaaacac tcatatttaa aatatcatgt atatttttat
    12121 ttttatattt aatagataaa gattcatcat taaatctttt tccctgacat tcactgcata
    12181 tatggtctat acttagatta tttcctaacc agaccttttc atatccacta ccactacatt
    12241 taggacaagc acctttggag ttaaatgaga aatgaccagc tgtaaatcct cgttctaacg
    12301 attctggctg acctgcaaat agtgtacgta tcttatccca tataccaata taagaagcag
    12361 gagttgagtt catatttttg ccaataggag attgagatat ctctgaatat ccagaaatat
    12421 gtttagtacc aatcagtcta tctgcaatag tttcaataac tgcataatcg tcttcatcac
    12481 ctatttcgct atctgaagaa tttatttgat tattgatagt atggttatta aattggcttc
    12541 taagaagagg aagtagggtg tctgatataa gtgaactctt gccactacct gattttccag
    12601 atattccaac catagcaccc aaaggtaatg atacagttac atttttaaga ttatttgtat
    12661 ttgcattttg aatagttata caaggtattt tatcagtatg tgtaatattt ttattgcttg
    12721 ttctattagg cattggatat tttccagata gatactgccc agtaagtgat ttgtcggatt
    12781 gaagtagacc ttctaaatct ccttgatata ctaattgacc accttcaaca ccagcttttg
    12841 gaccaatatc aataatatgt tctgccattt caattgtgtt tttatcgtgc tcaactacta
    12901 tgaccgtatt gcctaaatct ttaagtttct ttattgattt taagatgtct attttttctg
    12961 attcatgcaa tccagctgtt ggttcatcaa gcacataaat taatgagtcc atctctgaat
    13021 cgagatgtga atttaaaaat agtctttgca tttccccacc actcaaagtt gacatttctc
    13081 ggtataagga tagatgacct aatcgtgatt ctattaaatg tcgagttttg gataaaatat
    13141 catttacaag atttgtacca aatgtgttta aattaccttg gtttattaag attttaagga
    13201 aatcatcaat ttctaaaatc gtcatttttc caagttctcc aatatgtttt ccgtttaaaa
    13261 gtactctacg tgcttcttca ccaattttaa aaccattgca atctgaacag gcagtcatag
    13321 tataaactcc atttaaatcc tcaccccttt tgtaacgatt gtgaaatacc tttgataaag
    13381 aggaactttt tttaccattt tcataatgac catagagtac ttcatttctt atttcttcag
    13441 gtagttgtaa gaattgtatg tctaaataat cgttgaattt tttagaaaat actctcaaat
    13501 atcctggtgt aatctttgct ctatctaaga cttgttttag ggtatcatgc ttatcaagga
    13561 ctaatttttc aatattaatc tcaaaatatg caccatgtcc agagcacttc atacacattc
    13621 catctgaaga gttgtatgag aaataacttg gtcttaatcg ttcttcctca tacccacaat
    13681 tattgcaaat tagattatcg cttacaggag tatcacacat agtacacatt atttgacctt
    13741 ctactgaata caatagtgca agcatgttga gaatattggt tcttgtgcct actgttgaac
    13801 gaggattgct ctggcgaata atattttgtt gaacagctat agtgggagct aaaccttgaa
    13861 tattatcaaa cttatcctca ctatcaatac ctgcaagtat acctaaagat tgcagatatt
    13921 gtttacgacc ttcctcaaat acaatgtcaa acataaggct agacttacct gagccactga
    13981 cacctgttgc aacaacaagc ttatctttgg ggatagaaac atctatattt ttaagattgt
    14041 gtatctttgc tccattaatt tttatttctt tcattggttc acctcatttt atcaatttta
    14101 tcaaaccgta aaatagggat atattaatcc ttaatacaag aatagattag gtgaggttat
    14161 taaaaaggtt ctataggtaa atatatatcg acaaagtgtc tattttgagg gtgattgtgt
    14221 gggttgttaa gatatacttc aaaagggaag gaggcacgta gcttataatt accatttgat
    14281 agccattcat tatacataaa atcccacgca tcactatatt cagattttga aatatcaaaa
    14341 tgtcccacaa gatattttcc agaagggatt atcatattgc caagattgtc attctctttg
    14401 acaacaatgt tatctggaat ggtcatgcaa atactagtcc ttaattgatg ctcctgtgta
    14461 aattgtggat tatcatgata aatggctaat atttgtgtgt tttcaagtcc aagtagattc
    14521 tgctcacttg catatgtgaa taaccgttct aaaagttttg ggaaagtact ggttagatta
    14581 ccatatgaac cagtatatct agtgtaaata gtattcatat tatcaagttc tagaatttca
    14641 atttcacctt taacctctct agtattctta catttaggtt ttgatatgct tctattgtac
    14701 tgagaaattt tatatggttc tttgcaattc ttgctataat tgtttctata ttttataggg
    14761 cttattttat agtaattttt aaatgctctc gagaaaactg ctgaatcagt aaagccgaag
    14821 tagtgtgcaa tatcagtaac agtcatgtca gttcgatgaa taagaaaagc agttgcattt
    14881 tctagtttta gcctatttac atattgtgcc aaagtttctt tttctattgc tttgaagatt
    14941 ctatgaaaat gaaattttga aaaacctgct atattagaaa gttcatcaag agaaagaggt
    15001 tcatgtaaat gttcttctat atagtcttgc actttgcaaa tacgatttag atattctttt
    15061 ttacttttag catttgtcat atatagccct ccttattgtg taaaatgtgt attaattaat
    15121 ttatagcaat atagtaataa aatt
//
LOCUS       DAELXX010000097        15099 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.097, whole genome shotgun sequence.
ACCESSION   DAELXX010000097 DAELXX010000000
VERSION     DAELXX010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15099
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.097"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(238..774)
                     /locus_tag="KPH29_002837"
     CDS             complement(238..774)
                     /locus_tag="KPH29_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HBG1468419.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENKDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEEEN"
     gene            complement(792..2105)
                     /locus_tag="KPH29_002838"
     CDS             complement(792..2105)
                     /locus_tag="KPH29_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HBG1468420.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEENIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            2441..2881
                     /locus_tag="KPH29_002839"
     CDS             2441..2881
                     /locus_tag="KPH29_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBG1468421.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLETQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVSLTPKA
                     KELWVTRYTCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            complement(3066..3254)
                     /locus_tag="KPH29_002840"
     CDS             complement(3066..3254)
                     /locus_tag="KPH29_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468422.1"
                     /translation="MNKNNNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTVIKNSNKKE"
     gene            4315..4710
                     /locus_tag="KPH29_002841"
     CDS             4315..4710
                     /locus_tag="KPH29_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468423.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNNIKCEEIKDNVIEEKNNTCTTE"
     gene            4753..6852
                     /locus_tag="KPH29_002842"
     CDS             4753..6852
                     /locus_tag="KPH29_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBG1468424.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYTGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEISVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLKKDATEILVPISEIKEGD
                     LVSVTMGNMIPLDGVIVSGEAMVNQASLTGESLAVNKKEGSYIYAGTGIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNHSIAVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKKDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLREYGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIVLKQISNKLMKRVDLSSKFIIGFNLGLILLGVSGFVRPSTSAFLHNASTVGIS
                     LNSMTNLLENTNTYNYY"
     gene            complement(7077..12287)
                     /locus_tag="KPH29_002843"
     CDS             complement(7077..12287)
                     /locus_tag="KPH29_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HBG1468425.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRNFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKTLIGNVTEEKIRNILEKAKEDLDSQEKDYDGCKELVYL
                     LNVALSDGVSIKLCNPLDRQPEKLLDEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKTQLDKMYTDLCSRTNNMKELM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLCIPEGIYSINVSGK
                     DAEKLVGEGSIYDGMNGRNGNNAGTSGDILLSFKEIEKRGELKLYVDGGRGGNGQDGK
                     RGDTGITGEAGENAKIGSKKESKEFAKVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKASLLKGAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSAPIDDSDRNYYYFDRYVFNHASNENQVADDGEKLNALYEKHKDWTISKREDSGLV
                     GAEGKDGRSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILELSHIYEIFHAVEKIEMQLNREILQKYYSFEKKNKAVLSLDDMNIKKEFYKEA
                     KEIYEYLINKLMQSDRIESFIGEKKNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTQIESGNQQIEVLKDELENK
                     MLSVNAKMNLLIQEIIKMKEEEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSAKEEEYQWTNRMVDFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYAEKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSTKFGSLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSGSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYIDI
                     THGLDVKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNKKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSESIDILKSLGYIDLKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVDLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIC"
     gene            complement(12302..14425)
                     /locus_tag="KPH29_002844"
     CDS             complement(12302..14425)
                     /locus_tag="KPH29_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG1468426.1"
                     /translation="MQNSVKQEPCDFSHGRFRPEADILDSRLTKVLDLFQQLNMCLHK
                     DNFRGSITFFISKSFLGRTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFEELLKDNGK
                     NIHLCKPLESDEREGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIKEIQNNIVS
                     SLEDYVKAYTEDFISIIGSITMIQEIAKYDEVVKVFKNWLESTTTLTIERFINVLAKQ
                     SGLFLQDSCMELQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEKASKKIMEQ
                     NFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSILTNVTVTQ
                     NEELEDSITRLSAYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIRALIPNLVI
                     SSVLEYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMDFASEKNIILACNNLDVGSNVKI
                     NVSGKSGELVDSSMYGKNGEDGRDAGNIYLYIKEEIRNYRLTLIANGGNGSKGIDGKN
                     GETGDSGSNGADAGYTSSFHGQMFKGYFAKGTSGKKGDKGGAGGNAGRGGNAGNKGNI
                     YFISDSEEQKNRIINHCDITVKDGEAGPNGRPGSGGYGGSGGYHGYNTLSFSPSGIDK
                     TVYYTGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPAREYAEYGDRGDDGGFNAPNT
                     KLSTAVSKVEENLIKEKYQGYCSEI"
     gene            complement(14490..>15099)
                     /locus_tag="KPH29_002845"
     CDS             complement(14490..>15099)
                     /locus_tag="KPH29_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG1468427.1"
                     /translation="DIEAFENTNNQIGLDLGIKEFCISSCGDFIENPKYLKKSLNKLA
                     KLQRELSRKTIGSLNRNKARLKVARLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQ
                     VKNMIKNRKLSRLISDVSWSEFIRQLKYKANWHGRQIVKVGKFFASSQICNKCGYKNE
                     EVKDLSIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
BASE COUNT         4967 a         2729 c         1675 g         5728 t
ORIGIN      
        1 ttaaaaacga tataaatata caatttaata ctatttttaa caaataaatt tatattaaaa
       61 tacattaatt tatatagttt tatttattct aaaagtataa ttataaaaat aaaaaaagaa
      121 taggatattt ttcctactct tttttctgtg cttatatata atattaaagc tctttatttt
      181 caatatatat tttaaatttt ctctttttaa tatatctttt tcttttaata aatctcttta
      241 attttcttct tctatatcag agccaaaggg aatccatgca ttaccaaact ctttatcttt
      301 aaataaataa gctaatccag ttatttgctt caatctaaga atttcatcaa cttccatacc
      361 caggtgcttt gatatccatt catcagattt tccaatttca caaagttcag ttataatttt
      421 actcattaaa tccacattat gagaacctct tgcacgattg tgtcgaatag tacttgccat
      481 tctttcatca agagatttat caattactga aacaggaaga agtccgtttt ctctatcata
      541 tatatcctta ttttcaagca ttactctata tctatgaaaa ccatctacaa taatatactt
      601 atccttttcc tcattataat aacagacaat tggcattgta tacccatctt cttttatact
      661 tttatacaac aactgcattt ctggtggggc tactgcattt ggattatagt cgttagattc
      721 tatcttttcg attggcactg cgatgattcc atatacagga cttttatatt ccatagcatc
      781 ttaactccta tctaatgaat tttttcatat ttcttcttaa tatattccgt tctaattctc
      841 tgttcttttg taggaccaaa tcccataaat ctacacatat ggtcattttt taaaatacaa
      901 taacacattc ttttccaact tggtatatct ttggtaaatt taatatcatc cgtatcatca
      961 ggtatatcat ccaaaaatat gactctactc tttttatctt ttgtatgatt tgaaactcca
     1021 tttaattcta ttttataacc ttttctcaac aattcacgta taactatttc tgacaaacca
     1081 ccacctgtat tctgccaaaa ctcaatagat gttgaaaatt ttttaatata gctattcctt
     1141 atattttcag gtaaggtatc aagcaaaaaa tatgtgaaac ttttccatgt gtgtccatat
     1201 ggcaatttaa cactacgata tcccatagct tttgtttttc cataaataga agtaaaatta
     1261 actccttgta ctctagcaac caatttcacc catacatctg ggtccaatat tctgtataaa
     1321 tttaaactct cttttgctga atcattaaaa ggtgatgcca ctctcatttt tcttggagct
     1381 actccagctt tatagaataa gtcataaatt ttattatagt cataatcaaa tttatagtgg
     1441 gctaaccata tatcttctat ttcccaatca tatataggag atgccgtcca gacattttta
     1501 aattccttag ttatccagca ctttccttta tatccatgct gtttattcac aatagaacaa
     1561 taacgttgta gagattctgc tgctcgtgtt cctaataagc aaacaacttt tgcaccttca
     1621 tgtatatctc taatccatct accaaattgc tttgccaaac tttcttgtct cattttatac
     1681 ttataataag taaaaggatt atttttttga tttattatgt attctttgac tggcatttct
     1741 cttacccata attcttcttt ctcatcatcc catggatacc aatacatttc ataatttcct
     1801 acagcagttc ttgtagccat tggtaaacac acccaatatg gttcaatatc ttcaatattc
     1861 tcttcaaata ctttttcaac atattcagta gtcaaactat attgagcctc aaaatcttga
     1921 tgaaatactc ctattttgga acttatatta ttctttctct tataatccaa aactaaattt
     1981 agcagtaagc cactatcttt tcctccacta aaagatacat agatataatc aaattcatta
     2041 aaaagataag aaagtctttc ttgaaaagct tcaaatacat cggattctaa atatctctta
     2101 tccatttgaa ttatcccctt tcaattagaa gtttataaaa ccttacaata caatattatt
     2161 atgctttttt agcagtaatc agcataaatt atacattatt taaatacaca aatccaacta
     2221 aacacatata attataatgt aattatactt ttgtattata aactatattt tatcattatt
     2281 ttacattttt ttccaatata tataaatatc atatttttct attagctaaa aataattact
     2341 ttatattttt acatgttagt ataataagac ataaaaactt gacatactgt tgtcataatt
     2401 actttgctat aattaaaagt aaaaatggag gtaaattata atgtatgaac gtatgcttga
     2461 taaaagcaat aaaccctgtt tgtctcaaat aatagaatat ataggtaaag atggatatgc
     2521 acgattgtgt aagttggaaa cttacttgga aactcaatat aatttatcaa gagaacttcg
     2581 atttcctttt ggaaacaatt atggttgggg ttacaaatat agccataaat cttttcattt
     2641 attttatgct ttttttgaaa aaggtgcatt tactgttctg attcagatag gtgacaagca
     2701 agttccagca gtagaaaaaa cgttggtatc tttgacacca aaagctaaag aactctgggt
     2761 cactcgttat acttgtggca ataatggagg ttggattaag tatcgtattt tagaagatgc
     2821 agacttactt gatattattc aatatataag agcaaaaaaa tctccaccta aacaaaatta
     2881 acttttattt tttgtgattt aaatatagtg tatataaatt ccattctttc catattaaaa
     2941 aaataatata aaaaatatct attagtatat cttatttagt atgtcttatt ctacttttta
     3001 cagataatta aaaagtttta tcataaataa atcataacta aacatcctag taaatcaaaa
     3061 ttcatttatt cttttttatt agaatttttg ataacagtcc ctatacatat gccaattatc
     3121 attccaaaaa ttagaccata acacattgtg atacccacat ttcctttaga aatacctata
     3181 gcacaaccta tagccattcc aagtatcatg cctataaaca tacatataat aactaaattg
     3241 ttgtttttat tcatagtttt tctccattca atatatttta aattgcctag ctcgccttct
     3301 tagcttaaaa caaagaaata taaattaact ttctttcatt ctaatatctg cttgtataaa
     3361 tgtcaaacaa cacatttctt gtcataacct cctctttttg aagtaagtac aatctcccag
     3421 acacaataat atcaatttcc taaaaccatt taagtaactt ttatatttac ataatattta
     3481 caatcataaa ctatattgtt attcatttat acatcataaa cactccctcc taatcattat
     3541 tatacttaaa ataatgttca tttataataa tatatctaac gcactttaat tgaaaaaact
     3601 aaaactttgc ctttcattcc catatataaa taagtatact ttttgatgct ctattacaaa
     3661 tcaactaaat taattgatat aaattatcat tttctgttga aaataccatt tacacatgct
     3721 atacttaatt gcataaatgg taataaatgt catatgattt tacaaatcta ttaataacaa
     3781 ttattacttt tactgtattt taaagttttt ggactaaata tcaagaactt tctaaaatac
     3841 aaagtacaaa aaatttatat aagttataaa tttatatgaa ctagataagc tcaaacaata
     3901 tgttatgtat aaactgtgta gtattcggtt ataaatttat ataaattata taagctatat
     3961 aaagtattac ctcaactata taagctagat atataaatta catacataag ctagatatat
     4021 aaattgcata tataagctat ataagaatta tttcaactat ataaactaca tatataaatt
     4081 acatacataa gctagatata taaactagat atataaactg catatataag ctatataaaa
     4141 attatttcaa ctatataaac tacacatgta aaattcatgt aaatttacta atataaatca
     4201 aataaaatta ctaaactctc taaactaatt ttgtaaaaca tactctattt ttaacatttg
     4261 ttaccttata cataagtata aacaaaatct aactaaatga aaggataatg taaaatgaat
     4321 attttaaaaa tgaaaaaaac aggaatattt ttaggaggag tactttttgg tactgtaggt
     4381 gttaagatac tttcaagtga ctgtgtaaaa aaaacttgca ctcgtgcaac agcaggagca
     4441 ttgaaagtta aagattctgt tcttgaaaca actacgaaaa ttcaagaaaa tgtagaagat
     4501 attttagctg aagcaaaaga aatcaacgag caagaagact tatctacatc taaggtaaac
     4561 gttgtacaag atactcttaa atgcgaacaa gaagttctta aaactgaaac tataaaaact
     4621 gtagaagata ctaattctaa taacattaaa tgtgaagaaa tcaaagataa cgtaattgaa
     4681 gaaaaaaata atacttgtac aacagaataa atttaatgat tattatgatt aataatatca
     4741 ggaggaagta aattgaattt ttatataaaa cacgaaataa gaggtcgtat acgctttgac
     4801 ttacaacttg gcaaattaaa caacaagcag tccgatactc tcttatatta tttaacttct
     4861 ataaatggta ttaccaaagc aaaagtttac gatagaactg gtgatgcagt catttattac
     4921 actggtgaaa gagaatacat aataagagaa atcactaaat tcagttttaa caatagcaaa
     4981 atagaaaacc tagttcctga acataccagc agggaattaa acaattatta tagagacaaa
     5041 ttagcaaata aactgctagt caaaagtatc tccaaaatat tttttccaaa gtccttaaga
     5101 ttttttttaa ttggacttca ttctataaaa tattttaaaa aaggtctctc ttccctacta
     5161 aataaaaaaa tagaaatttc tgttttagat gccactgcta taggtatttc tatttttaga
     5221 aaagatatca atacggctgg ttcagttatg tttcttcttg gtatcggtga gttattagaa
     5281 gaatggacta gaaaaaaatc tatagacgac ttggctcaaa gtatgtcact aaacattgaa
     5341 aaagtatggt taaaaaaaga tgccactgaa atactagttc caatttcaga gataaaggaa
     5401 ggagatttag tcagtgtaac tatgggaaat atgattccat tggatggagt tatagtatca
     5461 ggagaagcaa tggtcaatca ggcatcactt acaggtgaat cccttgcagt taacaaaaaa
     5521 gaaggctctt acatatatgc tggaactgga atagagcaag gcaatattgt tatgtgcgtc
     5581 aaggaaaaag ctggtactac tagattccag aaaatagtaa ctatgattga agaatctgaa
     5641 aaactaaaat cttctgtaga gagtaaattt gaacatctag cagatacatt agttccatac
     5701 agctttttag gaagtatatt aacctatgca attactagaa accctataaa atcactctct
     5761 atattgatgg tagatttctc ttgtgcatta aaactctcta tccctatttc agtactatca
     5821 gccatgagag aatgtaacaa tcacagcatt gctgtaaaag gtggaaaatt ccttgaaggt
     5881 gttgcatgtg cagatactat tgtatttgat aagacaggca cattaacaaa agctcaacca
     5941 actgtttcag atataattac attccaaaat tataataaga aggatatgtt aagacttgca
     6001 gcttgcttag aagaacactt cccacactcc atagcaaatg cagtagttta tgaagctgaa
     6061 aaacaagggc ttagtcacaa agaaatgcat acagaggtag aatatattat tgctcatggc
     6121 atttcaacaa aggtcgatga taaaaaggtt gtaataggta gcaaacattt tgtatttgaa
     6181 gatgagaagt gtactatccc agacggtgag gaggataagt acaacaatct atcagatgag
     6241 tattctcacc tatttatggc aatatcgggt aaattatcag cagttatttg cataaatgac
     6301 cctttacgaa aagaagcaaa atatgtaatt tcaaatttaa gagaatatgg aataaaaaag
     6361 attgtgatga tgactggaga tagcgaaaaa acagctaaat ctatagcaag taaagttggt
     6421 gttgatgaat attattcaga agtcttacca gaagacaaag ccaattttgt taaaagtgaa
     6481 aaactaaaag gtcgcaaagt aattatgatt ggtgatggaa tcaatgattc tcctgctatt
     6541 tcagaatcag atgtcggaat tgcaatgagt gaaggtgctg aaatagcaag agagatttca
     6601 gatattacca tatcagcaga taatttgaat aatttaatcg ttttaaagca gattagtaat
     6661 aagcttatga aaagagttga tttaagttct aagtttatta tcggctttaa tttaggtctg
     6721 attttacttg gcgtaagtgg atttgttaga ccaagtacat ctgccttttt acacaacgca
     6781 tctactgttg gaataagttt aaacagtatg acaaatttat tggaaaacac caatacttat
     6841 aactactatt aatccccatt atataaaaca tatgtatata aaaaatttat aattcatctt
     6901 ttaaaaagaa actgtcttaa aaggagcttt tttctaatca aaattatata aaacatattt
     6961 tacaaatttt aaactcaact tatttctatt ttttacaaaa aggagatgat tcaaattttc
     7021 attttgaaac atcccctttt gtaaatttct ttaaaactta tatattaatt atattttcaa
     7081 caaataattg tattttcaac caatctttct tcatagcatt ttaaatcatt ttgctttaga
     7141 taattaaatt cagactctac ataaatacca cagcctacca attcaattgt caaattaggt
     7201 atattttcct ttagtatact agtctgctca taaataattc tttccaataa atcaacttgt
     7261 aatatagaaa aatgcttcat aatctcccca tcactatcta tatctttctt gatttcatac
     7321 caactctttg acaaaattcc cttcaaatca atatacccta gactcttcaa tatatctata
     7381 ctctcgctcc atgcatactt actaggttta tcaaagtatg attctagttt cttcttctgc
     7441 tcatttgtaa gtgaatcata agaaagcttc ttattaaaat gtgccttcaa agaaatctta
     7501 gccatagaat atggacttct accagagttt aatcctgcat ttttgaactt ttcaattcct
     7561 ttatcttcta ctgaagacca tattgagttt attgaatctg aatcattgta tttaacatca
     7621 agaccatgtg taatatctat atagttttgt ggaaatacat attgataata gacatctttt
     7681 ttatcatcat atagggagaa gtatccacta tcatataaat gaatatgcac ctttactttt
     7741 aaactatcct ctaaaatatt tcgtctaagg ttcgggatat ttgcatacat ggttacatat
     7801 tttatggcat caaagttttt atttaatgga acttcaaaac taatttcatc tcctgtcaaa
     7861 agctttttca cttcttcttc attacaatca tatagagaat atgtattttc aaatcccaca
     7921 cgtgatttcc catctaaact gcctgttaat gctaccttca aaatatggtt atccgttttt
     7981 tgccattggt aagcatcttg gtcaagaaac tctctaatct gtctattttg ctctttcgcc
     8041 tgatttataa cctcgttttc atcttctgta atacctacat ggcatctatt taagatattt
     8101 actatattgc ttccaaatgg aaacgtccat agttgaaaag ctgctgcctc aacctgacac
     8161 aaatatctaa cctgatttaa ttcaatttga tggataagtt ttttctgata ttcattcatc
     8221 tgattaccac tacctgacat ttgataaatc aattctccca ttgcaagatt atcttttttc
     8281 ttatcaatct tatcagtaat gataatttga gtttccatca tatcctgtac ttttttaaat
     8341 attatagaaa tctcctctga attttccatt tttaagcttc caaactttgt gcttaattca
     8401 gttaaaactt ccttcatctt taatttactt atctcatttt caaaaatatc ttgattttta
     8461 actgtactat cgaatttttc tacaaatggt ataacagatt ctttcaaagt tttatcttta
     8521 aattccacca tttgagtatt caattcatct aatttcttaa tattattttc tttatcttta
     8581 attgcctctt taactacctc tattttactc tcaaaactag attttacatt aagacaatta
     8641 ttaacaccac taatcgcagt atttataaga gaacaagcag aacttccaat actcaatgca
     8701 tttttaattt tagatgtctt ttgctgtctt tcaatagctt gtttactttt ctctagtgca
     8761 gctaacttat ctttttgctg tttattattg taatttgcct ccaacatacc tatttctttt
     8821 ttctggattt ttgtgtaaat ttccttatac tcgctttcac ttaacccctc ttttgctctt
     8881 tgaatatcgt cagctttttc tgcatagtta tcatcatcta tagacttaac tataatctcc
     8941 ttaatttggt caaatttagt tcttctttct tcatcaacac ctgccaaatt ataagtcatt
     9001 ttatctaaag cattttttct atcttcactt agctgttctc caagtacaaa tctcatacca
     9061 tcagaatcca atttaaaatc tgaaaaatca accatcctgt tagtccattg gtattcctct
     9121 tctttagctg aaagattttc cttcaaagat tttgcactgt caaatgcccc tagacaagct
     9181 tgaccaacac cactaaatgc agtcagtaaa ctcaatgcgc tttgtattcc agaaagaatt
     9241 aactgtgttt tcatttgttc ctcaagtttt gcctgtttct tttttagttc ctctatgtct
     9301 tcttgctcct cttctttcat ctttataatt tcttgaatca acaggttcat ctttgcatta
     9361 acagaaagca ttttattctc aagttcatct tttaaaactt ctatttgttg atttccagat
     9421 tcaatttgag ttaggatact ttccttataa gtattgaaat atccttcttt taactcatct
     9481 ttctttcctt gttccaattc tcttaaggtc ttgtccagat aatctgtcaa agaacgaata
     9541 tctgtaacac gatttcctcc aatttcactt tcttcaatca tctgcacata gaatgctgtt
     9601 ttttgttcaa gtgcaaataa aatcatactc cattcgctaa aattcttctt ttctcctata
     9661 aaagattcta ttctatcaga ctgcatcaat ttatttataa ggtattcata gatttccttt
     9721 gcctccttat aaaattcttt ctttatattc atatcatcca gagaaagcac tgctttattt
     9781 ttcttctcaa aactatagta cttctgaagt atttctctat ttaattgcat ctctattttc
     9841 tcaactgcat gaaaaatttc ataaatatgt gaaagttcta aaatatcagc ttctaaaatt
     9901 cccactgcac acaacagtct aagatttcta taacctttaa ttccagaaaa accaaatctc
     9961 tcgaaaaatt catagaagaa ggtatctatt gacaaagaat actcctgctt taacaattcc
    10021 ttttctttta atgcatctcc agagcgaccg tcttttccct ctgctccgac taaacctgaa
    10081 tcttcacgtt tgctaattgt ccagtcctta tgtttttcat aaagagcatt taatttctct
    10141 ccatcatctg ctacttggtt ctcatttgaa gcatgattaa acacatatct atcaaaataa
    10201 tagtaatttc tatcactgtc atctattgga gcagaggtac ctatatatct tattgtttct
    10261 tcatatatac ctctaattcc accaagacct cctgcaccac cagctcctcc agagcctcct
    10321 tttcctgcta gaccatgatt tccatctttt ttacttatag caccttttaa taaagaagct
    10381 tttccaagga gataaacctg tccagacttg ctcccaagac ctgcatttcc accattacct
    10441 cctgtgcctc cagttccacc ttgttttcct ttttctgctt ttaccacata gtattttgta
    10501 cctttaacct tagcaaattc ttttgactct tttttagaac ctattttagc attttcccca
    10561 gcttctccag ttatccctgt atctcctctt tttccatctt gtccatttcc accacgtcca
    10621 ccatctacgt aaagtttaag ttctcctctt ttttctattt ctttaaaaga taataaaata
    10681 tcacctgaag tacctgcatt attgccattt cttccattca ttccgtcgta aatagaacct
    10741 tctccaacca atttttctgc atccttacca gatacgttga ttgaataaat cccttctggt
    10801 atacataatt tttctgtaag tagtacaata ttcttacctg agaagacttc tttctccata
    10861 ttacagtcaa ttttcaactc acaatctcca tacatatata tagatgacaa aatattaagt
    10921 tgataaccag atattatttt agtcaaatcc gataaattca ctttaggtat aggaatatat
    10981 atagaaagta cattttcttt ctcatatgat tttatatatg tacaactttg aatcttctct
    11041 atcctatact ctgcatcttc aagctttttg gcttcttctt ccctatattc ttcttctgct
    11101 atccaatatt tgatatcttt taatgtatct tctaaagtat tttttagcaa tgattttaac
    11161 aactcactta gaccaacaat atgagtctca ctacagtagc tatttaactc taaaagaaga
    11221 cgtgtttctt ctaatatatt cactttctgt acgccatcac catatgaaag ttcttgtaca
    11281 aactccataa gctctttcat attattagtc ctactacata aatctgtata catcttatct
    11341 aattgagtct ttttatcctt tagactattt atagaagata gaatggaaat agaatcttcc
    11401 ataccttttt tgacactttc tatataagga actaaaagtt tttctaggtc tttataggtt
    11461 acatcaacgg ctttttgtat ttctatttta caagaagcag taagcatcaa tcccaaatct
    11521 gttttttcaa atgtttcagg tagatttttc atttcatcca acaacttttc tggctgtcta
    11581 tctagtggat tacataattt aatagataca ccatctgata atgctacatt taataagtac
    11641 actaattcct tacaaccgtc gtaatctttc tcttgactat ctaggtcttc ttttgctttt
    11701 tccaatatat tccttatttt ttcttctgtt acattaccga ttagtgtttt aggtatttta
    11761 ttgataagaa agaagaaatt ttctttatac tttgcataat caccaagcat atctttcaaa
    11821 tttttgcctg tttctatcaa taatgtatgt ctagagtcca gtaaggattg atagtctaaa
    11881 ataactacca tccctttcac ctttgaagat gtagaaatag tttcccttac aagcatagca
    11941 ccagcaatca tatactctgc tccacggtta tcaaaaaaac ctgggcaatc acataatgaa
    12001 aaatttctat ttgcaatatc tacaacttct ggacataaag tcttagaata tacaccattt
    12061 ccaatttcga cagctgaatc ttttgcagtt atatatattt gtccagtctc ttcatccgtt
    12121 ttttcttcca tcacgcaacc ttttaaataa tttatacaag tactctttcc aacgcctgaa
    12181 gcacctaaga ataacaaaat tgacttttct tttaagtcat attttctaag ctcgtctctt
    12241 ccttcttcca aaaggtttac gatttcacta taaagtttat ttgccataca tccatccctt
    12301 tctatatttc ggaacaatag ccttgatatt tttctttaat aagattctct tctacctttg
    12361 aaacagctgt tgataactta gtatttggag cattaaatcc tccgtcgtct cctctatctc
    12421 catattcagc gtattctctt gctggtcctg ttttgccttc attccaactt ccgccactaa
    12481 attctatatg atatttaatt atactatcta acttataatc ccaacttgta aaatatcctg
    12541 tatagtaaac agtcttatct ataccacttg gagaaaatga aagtgtatta tatccgtgat
    12601 agccaccact tcctccatat ccacctgaac ctggtctacc atttggacct gcttctccat
    12661 ctttcactgt tatatcacaa tgattaatta ttctattttt ttgttcctct gagtctgaaa
    12721 tgaagtaaat attaccttta ttaccagcat ttccacctct tccggcattt ccaccagctc
    12781 cacctttgtc acctttttta ccacttgtac cttttgcaaa atacccttta aacatctgtc
    12841 catggaaact acttgtatat cctgcatcag ctccattact tccagaatct cctgtttcac
    12901 catttttacc atctattcct ttagaaccat ttcctccatt tgcaatcagt gttaaacgat
    12961 aatttctaat ttcttcttta atatataagt aaatattacc tgcatctcta ccatcttcac
    13021 catttttccc atacatagaa ctatctacaa gttctccgct ctttccactt acattaattt
    13081 ttacattact acctacatca agattgttac aagcaagaat aatatttttc tctgatgcaa
    13141 agtccattcc aaggttatca tccatataaa tagtatcttt ggcaaagaac ataaatgtag
    13201 aaacattgtc atctactttt ctatttttga aatattccaa tacactacta attactaagt
    13261 ttggaattaa tgctcttatt tcaatacctt tcttatcatt aatattaatt gtttttacta
    13321 taaatggatt taaaatgtat ttaccaataa attctctaat tatctcatta ccttcttctc
    13381 catatgcact taatcttgtt atactgtctt ctaactcttc attttgtgta actgtaacat
    13441 ttgtcaaaat tgaattaaaa tcacttacac tatatacata atccttttta tttgcaactt
    13501 ccattaattt ttctctattt ggtaatgtaa atacctgttt ttgtacttca taagaaacta
    13561 aaactttttc taaattcttt cttattataa tgaagttttg ttccattatc ttcttacttg
    13621 ccttttctat aataaattcc atatgtacag aaggtaataa aatttcccca gttccatgtt
    13681 ctgtatattt acttaaagca tcaattatat ctaagtttcc ttttatctta ttttgtaatt
    13741 ccatgcaact atcttgtaag aataagccac tttgtttggc taatacgttg ataaatctct
    13801 ctatagtaag agttgttgta ctttccaacc agtttttaaa tacttttaca acctcatcat
    13861 attttgctat ttcttgaatc attgttatac ttccaataat actgataaaa tcttctgtat
    13921 acgccttaac ataatcttct aggctagata cgatattgtt ttgaatttct tttatcaacg
    13981 tatctaaaac taatcttgca tctgcactaa ttggatattg gaatgctgat gtcttatctt
    14041 taacttcatc taattctagt acttgcttta ataagccttc cctctcgtct gattcaagtg
    14101 gcttacaaag atgaatattt ttaccattat cttttagcaa ttcttcaaat aaccaagctt
    14161 tttcctctat attactatct aaattttcac ataattgttc atattttctt tttataacat
    14221 tatatatttg tttttctgtt cttcctaaga aactttttga aatgaaaaaa gttatactgc
    14281 ccctaaaatt gtctttatga agacacatat ttaactgttg aaacaggtct agtacttttg
    14341 taagtctaga atctaatata tctgcttctg gtctgaacct cccatgacta aagtcacagg
    14401 gttcctgctt cacagaattt tgcatactaa aatatgtctt actaattctc cacaggccat
    14461 ccccgtagtt cctacggttc ttacataagt tatttatttt gaattgttat tagtcttaat
    14521 ccttctttta atatatttat acttgcattt atatctctat cgtgagtttc attacagcta
    14581 ggacaaatcc attctcttat acttaagtcc ttcacttcct cgtttttata cccacattta
    14641 ttgcatattt gtgaacttgc aaagaattta cctaccttta caatttgtct accatgccaa
    14701 ttagccttgt attttaattg acgaataaat tctgaccatg atacatcaga aattaagcga
    14761 gatagtttac gatttttaat catattcttt acttgtaaat cttctatgca aataatatca
    14821 ttctctttta ttaatttagt agatagtttt tgtagaaaat catttctttg attggctata
    14881 tgttcttgaa gtcttgcaac ttttaatctt gctttatttc tatttaaact accgattgtt
    14941 tttctcgata attctctttg taatttagca agtttgttta atgacttctt aagatactta
    15001 ggattttcta taaagtctcc acaacttgag atacaaaatt ctttaattcc taaatctaaa
    15061 cctatctgat tattagtatt ttcaaatgct tcaatatct
//
LOCUS       DAELXX010000098        14908 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.098, whole genome shotgun sequence.
ACCESSION   DAELXX010000098 DAELXX010000000
VERSION     DAELXX010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14908
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.098"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            219..866
                     /locus_tag="KPH29_002846"
     CDS             219..866
                     /locus_tag="KPH29_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBG1468428.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTAFKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(1033..1602)
                     /locus_tag="KPH29_002847"
     CDS             complement(1033..1602)
                     /locus_tag="KPH29_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468429.1"
                     /translation="MKKFILCIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(1607..2506)
                     /locus_tag="KPH29_002848"
     CDS             complement(1607..2506)
                     /locus_tag="KPH29_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG1468430.1"
                     /translation="MKLLSKVNNNIRLIIQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIMFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGIPLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(2517..2666)
                     /locus_tag="KPH29_002849"
     CDS             complement(2517..2666)
                     /locus_tag="KPH29_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468431.1"
                     /translation="MSNFIKDNKKGISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(2659..3243)
                     /locus_tag="KPH29_002850"
     CDS             complement(2659..3243)
                     /locus_tag="KPH29_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBG1468432.1"
                     /translation="MKKFFMVVLGCIFVLGVISGCSKKESKPVEKNKSNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKNIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEEWKKIIDERLKMVKANE"
     gene            3664..4989
                     /gene="cdeA"
                     /locus_tag="KPH29_002851"
     CDS             3664..4989
                     /gene="cdeA"
                     /locus_tag="KPH29_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HBG1468433.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GISFTVISYFFIKEISILLGATDKLLPYCITYGKVMILCTPFYILKFIFNYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFVSSLLVFIIALVFA
                     PFIVRVFVSPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKKYKGRYKY"
     gene            complement(5084..5878)
                     /locus_tag="KPH29_002852"
     CDS             complement(5084..5878)
                     /locus_tag="KPH29_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1468434.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMVIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(5879..6754)
                     /locus_tag="KPH29_002853"
     CDS             complement(5879..6754)
                     /locus_tag="KPH29_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1468435.1"
                     /translation="MRSSIKIENLSHSFKKQNIFNNMSLNFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEEELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSENDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(6758..7126)
                     /locus_tag="KPH29_002854"
     CDS             complement(6758..7126)
                     /locus_tag="KPH29_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG1468436.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(7309..7968)
                     /gene="deoC"
                     /locus_tag="KPH29_002855"
     CDS             complement(7309..7968)
                     /gene="deoC"
                     /locus_tag="KPH29_002855"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBG1468437.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(8004..9548)
                     /gene="murJ"
                     /locus_tag="KPH29_002856"
     CDS             complement(8004..9548)
                     /gene="murJ"
                     /locus_tag="KPH29_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBG1468438.1"
                     /translation="MSKVAKATFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTKILITGIIFIGITSVMSAFLQIKENFIVVGF
                     GSIPYNIVIIISIMLSTVFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKD
                     DSLIKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYSDRLNGFVTGTFTA
                     SIVSVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFER
                     GAFDARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVL
                     DLVLIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIM
                     GVLSYFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMTIFKVEEVDMILNIAKR
                     KLHLKK"
     gene            complement(10022..10906)
                     /locus_tag="KPH29_002857"
     CDS             complement(10022..10906)
                     /locus_tag="KPH29_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG1468439.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
     gene            complement(11197..11673)
                     /locus_tag="KPH29_002858"
     CDS             complement(11197..11673)
                     /locus_tag="KPH29_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HBG1468440.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGVLDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQAKDLEEHHHHDKNCGCGCNHHHEN"
     gene            11940..13115
                     /locus_tag="KPH29_002859"
     CDS             11940..13115
                     /locus_tag="KPH29_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HBG1468441.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(13196..13999)
                     /gene="proC"
                     /locus_tag="KPH29_002860"
     CDS             complement(13196..13999)
                     /gene="proC"
                     /locus_tag="KPH29_002860"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBG1468442.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINNEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(14039..14623)
                     /locus_tag="KPH29_002861"
     CDS             complement(14039..14623)
                     /locus_tag="KPH29_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1468443.1"
                     /translation="MNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLTSV
                     ATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLKEK
                     TMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYNSE
                     KNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
BASE COUNT         5050 a         2442 c         1657 g         5759 t
ORIGIN      
        1 attatgtttt ataagtattt ctttattcag ttatttgtta aaaactaata gtaaatctta
       61 tactaatcgc atatttatgt tataactata ttgtaactat tttcgtaatc ttaggaaaag
      121 attacttata aaattaacct tatacaataa tatatcgtat aatcttaatt agcataatag
      181 ttacaatatt aaaatttaaa tatgggagtg attttagtat gataagacaa acaccattag
      241 agaaacgata tgacaagcta ggtcctcata ttgtaacagc attcaaaaaa cgttattttg
      301 atgcttatta ctgtaaaact agaaatgaag ctttgcaaca aattttagat ttaattcctc
      361 aaaatcatgt agtatcatgg ggaggatctg aaacacttaa agaaatgggc gtacaaactg
      421 ctgttagaga gaaaggattt aaagttattg atagagattt agcaaaaact ccagaagaaa
      481 gaactgaaat tatgcgtcaa gcacttctat gcgatacttt tttgatgagt agcaatgcaa
      541 ttagtgaaga tggacaactc tttaatattg atggaaatgg caatcgtgta gctgcaatga
      601 tatttggtcc aaaaaatgtt atagttgtag ctggtatgaa taaaattgta aaaaacattg
      661 atgatgcaat acaaagaact agaacaattg catctccagc agttgttcag cgttttcaag
      721 atgtcaatac accatgctat attaatggaa gttgtataga ttgcacaagt cctgaaagca
      781 tttgtgcata catggttact acaagaatca gtcgtccagc aaaaaaaata aaggtaatat
      841 tggtaggaga aaatcttggt ctataaaata tacatacatg taaatagctc tataaataat
      901 aaaatcatat gaaataactt atttctaaaa gtggtagaat aaaagcttct acaagacttt
      961 cattctacca cttttatatt cttttaattt gtgtttctat attattaatt gctccaattt
     1021 tcatatatat tactaactat ttttattttc ataatttttt tgttcttcat caagctgttt
     1081 ataataatct ttattatagt cagctagata atctatacca cctcctgtaa tcacatttaa
     1141 taatttcatt gcagattctt gattaacttt acaaacataa ttatcaccag atttaacaag
     1201 ctctaatgta atattaaatt cttttttctt tgcagaatca atatctttca tagttggatt
     1261 tggcatcatt tctcctagtt taggaggatt cttttttaaa atagaaacca tatcagtatt
     1321 gttaattttt aaatctacag tcactttatc cttattattc tttatatctg aaatttccca
     1381 agtcatattt ttagaataca ttttaaatcc ttctttatct tcgtatataa gattacctaa
     1441 ccctgatttt gggaaatact tatcaacagt atctgcatct tgtgttttta ttgaattaaa
     1501 catactatcc acttcatttt ttatcttatt atcattagta caaccagcta acaccagtac
     1561 tgatattgca aacattataa tacatagaat aaatttcttc attaaattat atccccctat
     1621 acttttttgc acaaatatta ttctcttttt tatcttttga aaaagttgta gtaattgcat
     1681 tttgtggaca aacttttata cattcaccac acctaataca gtctggacta tttacatctt
     1741 tatatattgg tatatcaagc ttacattttg tttgacatgc tgtacaattt gtacatttat
     1801 ctttatcaat tttaaatcta tatattgaaa taggattaaa aaaaccatag attgctccta
     1861 aagggcatat atacttgcaa aatggcctat aagtaatgat tgacatcaat aatatcaata
     1921 gcaaaatact taacttccag ctaaataaaa atcctattga ttctcttaaa ttctcatttc
     1981 ctaaaataag tggtattcct cccaaaagtg ttcctgaagg gcaaatccat ttacaaaacc
     2041 aaggatttcc accccctgct acatttacaa tagcaactgg caaaataatt acaaacatta
     2101 ttaagactat atactttaga tattttagat atttatcacc ataaactttt tttatcttta
     2161 ctggaaaggg tactttatgt agtaaatctt gtacaagtcc aaagggacaa agccaaccac
     2221 aaacaaatct gccaaaaaaa gcaccaaatg ccattaaaaa accaacaaca taaaatgaga
     2281 atttatactc tctgcttgat aaaactgctt gtaatgaacc tattggacat gctccaagtg
     2341 cacctggaca tgaataacaa ttcaatccag gtacacataa ttttttactt tctcctccat
     2401 atatagttcc ttctaaaaat ccatttacat atccatttgt taatgctgta aatgcaactt
     2461 gaataataag tcttatatta ttatttacct tagataataa cttcattcct cttctcctaa
     2521 ccaataccaa tacactcaag acaaatcata attgctttag ttaatactgt atttgtttct
     2581 ccacgatatg tacctaatgc aataaatata atactaattg caataataga aatgcctttt
     2641 ttattatctt taataaagtt actcattggc ttttaccatt tttagtcttt catctataat
     2701 ttttttccac tcttccttac tatttgcacc tacaaccttc tcacccacaa agtttccttt
     2761 cttgtctaca aaataagttg ttggaaactc cataactctt ttaaaatcag tttttttaag
     2821 ttcttcagat acagtcaatt gttggtaagt tgcatctgac tttttcataa tgtttttaac
     2881 taaatttttc tcttcatttg aaagaccagt tctcatatca gttttatcaa cttctacaac
     2941 taatcccttt atagctacat tggaattatt actctcatat tcttttgata atgcttcaag
     3001 ctctggcatt tcacccacac atggtccaca ccaagtagtc cagacattaa taagagtaag
     3061 ttctttattt gaaaatatct cttttgttac cttatttcca tcaacatcct ccgtatctaa
     3121 attactaaac cattcactat tactcttgtt tttttctact ggcttagatt cttttttgct
     3181 acatccagaa atcacaccta aaacaaaaat acatcctaaa actaccataa aaaacttttt
     3241 catatctcac actcctagaa tttatatagt atataaaatg gctgcttgaa ataaaaataa
     3301 cttctcttct atattaaaac attatattta ataaataaga actaatttaa aataagttgt
     3361 ataaaagata tatagttaaa atctatttct tttcagacaa aatcttatat atatataata
     3421 acttataaag cttaagatta tatacatata tttctaaaaa ttttcttaag aaacagttat
     3481 tccttactaa tgcaatatac ctatttttac taatataagt ttattaatta gcattatact
     3541 aatttatctt aatatgattt tttgttgatt ttaagctgtt tatcgtataa ataaaatttt
     3601 tcttgacata tagttactat atagcttatt atagatagag agtaataaaa ggaggtaaaa
     3661 tttatggaaa atttatttac aagaaaattc actacttttg aatttctaaa atttgtttct
     3721 cctgcaatta tatccatgat atttatatct ttgtacacaa taatagatgg catctttgta
     3781 tcaacattag ttggttccga tgctcttgct agtataaata ttgtactacc tataattaac
     3841 cttgtttgtg gatttggaat aatgatggca acgggtggag gtgctatcgt ttctatacgt
     3901 atgggtgaaa atagacagga tgaagccaac tctacatttt cttttatagt tttgttttca
     3961 ttgattgttg ggatttcatt cacagtaatc tcatatttct ttatcaaaga aatatctatt
     4021 ttgcttggtg caacagataa gttattacca tattgtataa cttatggtaa agttatgatt
     4081 ttatgtacac cattttatat tttaaaattt atatttaact actttgcaag aactgatgga
     4141 aattctaaat ttagtttatt tctatcagtc attggtggtg taacaaatat aatattggat
     4201 tatgtattta ttaaatattt tggaatgggt cttttaggag ctgcagttgc aactgctata
     4261 ggtattattt taacttgtgt tttaggtatt atttacttct tatctaataa atctacactc
     4321 aaattaagaa aaccaaaaac cgattttaga cttataagag atactatgat aaacggttct
     4381 tctgaaatgg ttacagaatt atctacagga attacaacat tcttatttaa tgtagtagct
     4441 ttaaaattag caggagaaaa tggacttgct gctcttacta tagtattgta tgctcatttt
     4501 ttaatgacat cagtctatct aggattcgct gctggagtgt ctccattaat aagctataat
     4561 tttggtgctg aaaacagtga taaattaaaa gaaacattta aacattctct aaaatttata
     4621 tttgtttctt ctcttttagt gtttattatt gctttagtat ttgcaccatt tattgttagg
     4681 gtttttgtaa gtccagataa cacagtattt aaactagcct tacaaggatt aaaaatattt
     4741 gcatttgctt ttttgtttgt tggtataaat atatttgcat caggattttt tacagcattt
     4801 cacaatggaa aaatttcagc tattatatct tttagtcgtg cctttgtttt tataatcata
     4861 ggaatcataa ttcttcctcc tatgttaaac atgactggat tatggcttac agttccattt
     4921 gctgaggtta taaccatatt tatatctatt ctatttataa aaaaatataa aggtagatat
     4981 aagtattaaa aattaatgct taaaatcaac atttattaaa actaataaac ctgctttaaa
     5041 atacaaattt acaaatttga tttttaagca ggttttattt tagttataat aagtcatttg
     5101 catactcttt aattggtgct tttctaagta ataaaattat aaatattatg caaataacta
     5161 taattcctac ctttgttcca aataaaataa aaggattctg aaatgaataa ataatttttt
     5221 ctaaatacaa tagcatttta tgcatatttt tagttgcaaa attaactata taaattacca
     5281 ttaatatcgg taatcctatc aatatagtaa tactagtaac ttttctaact ctatatgtaa
     5341 aagctccaag taatactcct ataaaaccta tacatatttc atttatacat atttgtacaa
     5401 aataatttaa ataagttagt ggcatacttg atgttgaagg atctatcagt gctgatatag
     5461 atgtccaaat catatctctc tttagtaaaa ctgatagttc tatactgtaa cctgttataa
     5521 tttctataaa taacttcgat aaaaaaatca aaactatacc taaaatagaa atcacaaagc
     5581 ttaaaataat taaatctatg acaatagctt ttatacaact ttttcgatct gctcttatac
     5641 ttaaagctcc tgaaaagtct ttatttgcta tatttactcc atacacaaat agcagtatag
     5701 ttatatatgt taaaatagga ttcacaatcg tggataaatt agttttagat gcttctccaa
     5761 aaatcttgag gataaacact ggcaaaatat tcataacaat tcccacaatc ataaatataa
     5821 ttatatacaa tttagtattt ttattaaaaa attttatata tggctttaat tcattcaatt
     5881 acaattcctc cttcttagtc atgttaacaa acatatcttg tagccctatg tatcctacat
     5941 caatatttga atttttaagt attttttcat cattttcact aaaatcacca tagtaaaaat
     6001 aagcaactgc atttccaaat gcctttttag gttttatatt ttttatacat tctaattttt
     6061 ctaactcttc tttatttcca gtaatataat gagctttttg ttttatgaca tcaatttctt
     6121 catctataat aatttttccc tcatttaata ttattacatg ctccagtaaa tcatctattt
     6181 catcaatcaa atgagttgat attattattg tccttggatt ttttatataa ctatctaaaa
     6241 ttatgttata aaactcttgt ctatttactg catccaaacc aattgttggc tcatcaaata
     6301 tggtgatagc tgaattagaa caaataccta ttatatttga aagtatagtt ttcatacctc
     6361 ttgacagttg tttatacttt tttttagtat ttaaatcaaa atatttgcat aattcttctt
     6421 ctaagccttt atcataactt ttataaaaac tagaagaaaa ttcaaatatc tgacctactt
     6481 taaaatctgg actataaaat tctttttccc tgacgataca aacttcttcc aaaactttta
     6541 tatcttcttt tgggttcttt cctaatattt ctatctcacc ttcattttga attaactgat
     6601 ttaccattat atttaataat gttgtttttc cagctccatt cttacctaat aaaccatata
     6661 tcttattttt ttcaaagttt agagacatat tattaaaaat attttgcttc ttaaaactgt
     6721 gagataaatt ttctatctta atagaactcc tcatatatta atcctccttg aaattaatta
     6781 taatatcaac caattcttct ctatttattt ctaatttgtt agcttcttga agtaggtttt
     6841 ttacaaaatt atgtttaaaa ttttcttttc tagcttcttt tataatagtt tttgcaccat
     6901 ctgatacaaa catccctatc cccctcttct tatataaaat atttttatca accaatatat
     6961 ttataccttt taatactgta gctggattaa ttttataaag ctttgaaagc tctgttgttg
     7021 atggaacctg ttcttcttct tttattccat ttgatagtat ctcatcttca attgctcgtg
     7081 ctatctgaat aaatataggt tcttcattat ttaatactaa tttcataggt tcctcctttc
     7141 tccattagtt agttactcaa ctaattaact atataactta taatatcttg aaaaatattt
     7201 tctgtcaata ctaaataaaa aacagaggga aaattccctc tgttttttgt tctttatatt
     7261 tataattaaa ttttgatttt tgtaatccta tcccataagt taaaaacttt aataatctgt
     7321 tgtatcttca ttttttaata ttttacatat tgagctagtt cctaaccttt ctgctcctgc
     7381 ctctataaat ttttctgcat cagaaattga tttaactccc cctgcagctt ttatttttac
     7441 attttttcca acatgctctt tcataagctt tatgtcttct aaagtagctc cacctgttcc
     7501 cattccagta gatgttttta taaaatctga acctgacatt gttactattt cacacatttt
     7561 tattttttca tcttcatcta aataacatgt ctctataatg acttttaata ctttatctcc
     7621 tatagctttt tttatttctt ttatttcttt tcctatatta tcataatctt tattttttat
     7681 atctgaaatg tttacaacca tatcaacttc atctgctcca ttttcaatag catcttttgc
     7741 ttcaaatact tttgtagcag tagtttgata tcccaaaggg aaccctatca ctgtacatat
     7801 ctttatttta ccttttagat attctgatgc tcttttcaca tatgatggtg gtatgcaaac
     7861 tgatgctaca ctcatatcta cagcttcatc acataaaatt ttaatatctt cccatgtagt
     7921 tgtagctttt aaaatagtat ggtctactgt ttttaatata tgtttcataa aatcctccta
     7981 aatttataat taatataatt agattacttt tttaaatgca attttctctt tgcaatattc
     8041 agtatcatat caacctcttc aactttaaaa atagtcatta gtagtgtata aattccacct
     8101 ccaactataa ctgaaatagc tagtgataca aactcattaa atgttcctac tcccaaaatt
     8161 ccaaatataa atttatacgt aaagtatgat aaaacaccca taattaaaga tgcaactacc
     8221 gatttcaaag ttgcttttat tattttatct tgtccaaaat atccaacttt tctctttagg
     8281 ttcaagaaga gtaacaatat acatgcaatt gatgatgaac tagttgcaag agcaagacca
     8341 ccatgagcca ttggttttat taaaactaag tccaatacta tatttacccc tacagatata
     8401 atcccattta ccataggtgt ttttgtgtct tgtaatgaat aaaaaacctt tcccaaaata
     8461 tctcttaatc caaatgcagt cataccaact gcataaaaaa ttaaggcagt tgctgtcatt
     8521 tgggttgccc tcgcatcaaa agcacctctt tcaaaaatga ttctaacaac aggagttgca
     8581 aaaaaaatag aagcaactga aataggaatc atagaaatta ttatcatatt tatactagat
     8641 ttaactgaac tagtaaactt cttttgatta ttttctgcac ttaacttaga taacatagga
     8701 tacataacag atactattga agctgtaaat gtccctgtaa caaaaccatt taatctatct
     8761 gaataagtaa gtgctggaat gcttccttta actaatgttg aggctaaagt tgtatctacc
     8821 aatgagttca cttgattaac agcaacacct ataaatactg gtgaaagtaa tgctagtaat
     8881 ttaataaggc tatcatcttt aaagtttaag tagtataagt atttgtagtt cgttttttta
     8941 acaaaaaaca tgtaaaagag cagttgaact accatagcaa ccacagcacc aattggaagt
     9001 atgtatggtc caaatacggt acttaacatt attgaaataa ttattactat attatagggt
     9061 atactcccaa atccaactac aataaagttc tctttaattt gcagaaatgc tgacataaca
     9121 ctcgtaatac ctatgaatat tattcctgtt atcaatattt tagtaaattt tacggtaagt
     9181 aaaaacctct ctccttcaaa tcctatagca aatatactta caagttgctt tgagaaaata
     9241 actcctaata tagctacaac tatgcatata ccaactatta tatttaaaac attgtttaaa
     9301 aactttagtg cttgcttttc accttgttta ctacttatat cttgatacat tggaataaaa
     9361 gtagtaacta tagcagtacc tattgctgca aatataatat ttggtatatt catagctgtt
     9421 aaataactct ctgtatataa gcctgtacca taaattgatg ataatactaa ttctctaccc
     9481 attccaagaa tcttagagat tatagtaact atcatcaaat aaaaagttgc tttagctacc
     9541 ttgctcattt tatcactcca cttctaacat acctcttttt aatcaagtaa aatttggctc
     9601 attagatata attctatcaa ctaatttgcc ttgcttagcc ctatcaaaat ataattaaca
     9661 cctatttctt aatatatcac aaactacatt attcttcata tttttttatt tctccaataa
     9721 gttttacatc tttcctatat aaattgtata gcttcaatta tattcaaaga aatggagttg
     9781 ctacccgata actttaaaag taaaaaacag attttattat ctttttaaac tatattctaa
     9841 catattttgt ccatattgag aaattttact aaactattat gtactttatt aaataaataa
     9901 aataacagca cacctataaa atacaagtgt gctgttcata aacagatttt ataaaaaaat
     9961 aattcctaag tagagattaa catgctttct catactctca tatataatgt gtctaaaaat
    10021 attaataaaa atgctttaat tgttttaact tacttggatg tcttaatttt cttattgctt
    10081 tagcttctat ctgtcttatt cgttctctgg taactccaaa tacctttcca atctgttcta
    10141 gtgttttagg gtcatcatca tcaattccaa atctcattct taaaacttgc tgttcttgct
    10201 caccaagacc tgtaagtagt tcttctatct tttcttttaa attatgctgt tcaacttcgt
    10261 gtattacaac atctttaaaa tcagcatctg atggtataaa ttcaactaaa ctactatcct
    10321 catcttcttt taatggagta tccaaagata caacattttt atctcttctt aataattcca
    10381 acactttgtc aacttctaag ccacatgcat ctgcaatctc atctatagtt ggctctctta
    10441 ataatacatt taaaagttct tgtttctttt tctttacaaa atttattctc tgatgaagat
    10501 gtataggtat cctaatagta ttttcgcaat cgtcgatata tcttgttata ctttgtttta
    10561 tccaccaagt tgcataagtg ctaaatttgt atcctttttt ataatcgtac ttttctacag
    10621 cttttataag tcctatattt cctgcttgaa ttaaatccaa catatcaata ctatctcttt
    10681 tatatctctt tgctatgctc actaccaacc tataatttga atttataaat ttttcttttg
    10741 ctactgaaga tgagttgatt atttccttag ccagctctac ctcttctcct gctgacaaca
    10801 ttttgtattc ctctatttcc tttaaataca ttttcatcgc atttgataca tttgatgaac
    10861 ccacacaaat ataattttca taattatttt cttttttagt atccataatc aaaccccttc
    10921 tattcataga atttgctaaa acaaaaataa ataatttaaa cctacataat ctacaattac
    10981 ataatatccc attctttaaa tgaattgttt tgtatattat gtatacaaat ttataaaaac
    11041 tatacctata atattattct acttctaacc gcaatatcct ccttatatat ataatttatt
    11101 ttattatatg ttgttatatt cttagttttt tgaatgatat atgtctaaaa aaagactgtt
    11161 tactaaacag tcttttttta tatatatgct caatatttaa ttttcatgat gatgattaca
    11221 tccacatcca caatttttat catggtgatg atgttcttct aaatcttttg cctgttgttc
    11281 catttcatga aactctttta ccattatatc ataagttgga agagctgcct cagtatcgta
    11341 ttcttcccct ctccaatcag cgtacatcat agcatcccca ttagttaaaa ccaatctacc
    11401 tgacatagtt atattatcca cttcttctaa aaattctatt tgtttttgta aaacttctcc
    11461 ttctaactct acatcttcat ctaaacattg cactatcatt acaggtgcat aataatcttc
    11521 ttcatcttta acatttacaa caatatctaa aactccttct gcaccttctt caaattctgc
    11581 caactcaaca tttgtatcaa tcatatcttg agggactaat aaatcttcta ttaaatattt
    11641 tattactcta tcttttacta attcttttga catttatttt tctccttccg attctataac
    11701 attatcataa tcatttctat attatagtat actacattaa ttgttttttc aatctcttac
    11761 aaaaattatt tttttattat cttccttata ttcccttttt tattacaaag ttgtaatcgc
    11821 attgatttaa aaaatagttt atatttagta taatagaaat gtagcacata tttttattca
    11881 atatattaat aaaaaaatag attgaaaatg taatgtatgt taaatctagg aggaatatta
    11941 tgaagtttaa aaaacaaatt agttctattg ttgtgtctac tatgcttgta cttgggagta
    12001 tgaacctttc ttatgcagat ggtacaaatg tagttacact tggagctaac ttatcagagt
    12061 ctcaaaagca gcaaatgctt aattactttg gagtaaaaaa agaccaagtc ttagttttag
    12121 atgtaacaaa tgcagaagaa agacagtatt tacaaggtgt tgcaacagag ggtcaacttg
    12181 gaaaagttac tatctcttgc tcatacgttg aacctactaa aaaaggaaat ggaattaatg
    12241 ttaaaacagc aaacttaact tgggtaacta gttcaatgat tgcttcaacc cttacaacag
    12301 ctggtcttga agatgcaaat gttattgctg ctgctccata tcctgtaagt ggtactggag
    12361 cattgacagg aatcatgaaa gcatttgagg atgcttcagg aaagaaactt gatgaaacta
    12421 agaaagaaat tgcttctgaa gaattggtag ttacaggtga cttaggagat gatatagggc
    12481 aagaaaaagc aacaggtgta atgaatgata taaaaacaga aattgtaaag aatggtacaa
    12541 aagataccaa tcaaatagca aatacaataa ataatgttgt aaataattac aatattacta
    12601 ttacaccaga acaaaaagaa caaattaaag gtgtaatgaa aaaaatagct gaccaaaatt
    12661 atgattacaa tagcatgaaa aatacactag ataatgtatc agataatgta aatgaacaat
    12721 taaagaaatt aggtgaaagt gttaagggtt ctggtatact agatactatt ggtggatggt
    12781 ttagtggaat aggagactgg ttctctggtt tattctcagg cggagataaa aaagatttgg
    12841 gaatcttaaa taacactaat gatgagtctc ttggctctga tgctgtaata aattctacag
    12901 aaggtgttca aataaatcca caaaataata ctaattctaa caatgatagt gatacaaata
    12961 gctcaaaaaa tgatacttca agtaataatg aaaataacga tagttctcaa acaaatgaaa
    13021 acaacacaac aaataattca gaaaatcaat caaataataa tgactcagtt ggtttctttg
    13081 aaaaaataaa aaattggctt tcaggcttat tttagaaatt aaacaaataa aaagaattgc
    13141 ctaaaatgga aattttctat aaaaggcaat tctctttatt tacaataaat ttttattact
    13201 cactcatttt tttagatttt tctatacatg cacatattgc ttttatcaca gaagctctaa
    13261 aaccttcttc ttcaagtact ttaactgctt ctatagtagt tccaccaggt gaacatacca
    13321 tatcttttaa ttcaccagga tgttttcctg tctcaagtac catttttgca gaacccataa
    13381 cagcctgtga tgcaaactta tatgcttgtg gtcttggcat tcctgcaatt actgctgcat
    13441 ctgctatagc ttctataaat aaaaatacat atgctggtgc agaaccactt gcaccaatta
    13501 cagcttctat aagatactct ggtactacct cagtatttcc aaaagaatta aatacttctg
    13561 taactgcttc taagtcttca ttgtttatat ttttatttat agaaagagaa gacatggctt
    13621 catttacaag agctggagta tttggcatag ttctaactat ttttttatct tctcctataa
    13681 ttgactctat atcctttata gattttcctg ctgctattgt aactataatt ttattgtcat
    13741 ctataaagtc ttttatctct tccaaaatat catcatatat atctggttta acagctacaa
    13801 ttactatatc agaggttcta gttacttcct tcgagtcttt tgtagtattt attccaaaat
    13861 ttgcatctat tctatcgaga gttccttgac tatatgctga tgctataacc atattaggtt
    13921 caactaattt tgaatttact attcccccta tcatagcgct tgccatattt cctgcaccta
    13981 tgaatccaat ttttttcact ttagtacctc cttattgact aatagtaatc gttaaaattt
    14041 aagcttcttt aactgcatca gacttttcaa atggatttgg tatacttact accaaaattt
    14101 tcactacctt attagtatag ttgacccaat tatgattgtt cttttcacta ttatagtgta
    14161 ttgtgtctcc tggatacatt tctatttgtt catctcccaa aaacactgta agagtaccct
    14221 ctaatacata cacaaattcc tcaccttcat gtacgtaaca acaaatttcc tcactactct
    14281 tacttggaag aatttcaatt aatcttggta gcattgtttt ttcctttaaa ctagatgata
    14341 aatgataatg tataaatgtt gagttttcta catcaaatac ttctttttca taacttctta
    14401 atacagctct tttatgctct tttggcttca taaaaaagta agttaattct acatctagta
    14461 cacttgctat ttttcctaag gaatctgttg ccacacttgt taatcctctt tcaagttgtg
    14521 ataaaaaacc aattgacaag tttgtttttt cactcatatc cttcagcgtc atttgctttt
    14581 gagttctgag ttgctttatt tttgctccta tgtccttatt caaaaccatc acccctaaat
    14641 aattttaatt ttttcataat ataaatacaa aaatataaat ataggtactg aacctatctt
    14701 tatttcattt aaaaaaattt ttataagtat tactataatt tatcaatctt tttttgtcta
    14761 catttttacg attatttacg atttattttt cacatatgta tatttaatct tgccaactaa
    14821 agatacattt tttataagca tttactcttt atataataaa acttaagttg ttcatgtagt
    14881 cttcttctgg tttaattatt aaaatttc
//
LOCUS       DAELXX010000099        14807 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.099, whole genome shotgun sequence.
ACCESSION   DAELXX010000099 DAELXX010000000
VERSION     DAELXX010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14807
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.099"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            846..2516
                     /locus_tag="KPH29_002862"
     CDS             846..2516
                     /locus_tag="KPH29_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HBG1468444.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYEEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHRMNYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESNKYYCTVTAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
     gene            complement(2638..3372)
                     /gene="pflA"
                     /locus_tag="KPH29_002863"
     CDS             complement(2638..3372)
                     /gene="pflA"
                     /locus_tag="KPH29_002863"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBG1468445.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNTDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(3532..5763)
                     /gene="pflB"
                     /locus_tag="KPH29_002864"
     CDS             complement(3532..5763)
                     /gene="pflB"
                     /locus_tag="KPH29_002864"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBG1468446.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPAALGKDMTERINNLSAMMDGYFS
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            6056..7117
                     /locus_tag="KPH29_002865"
     CDS             6056..7117
                     /locus_tag="KPH29_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBG1468447.1"
                     /translation="MEKRKSIHLHKLNIYLIGGGDRLFITVVLFLVGFLLITKGADIF
                     INCTVEIGRKTNISEIILGATIVSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICN
                     TGLALGLVVFISPFNVDKKMFFSKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIF
                     YMVNNYRSVVGKSDTKNRNIKNNTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMM
                     IIGSQILIESGVIIASFLNIPQGIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNI
                     LGANILNIVSVIAVSAIPNNIPILSQNRQLDIPFMILLLLIVIIPTLKSNRLSRIQGI
                     LMLFTYFLYISILYFMYII"
     gene            7208..8821
                     /locus_tag="KPH29_002866"
     CDS             7208..8821
                     /locus_tag="KPH29_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG1468448.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            9096..11081
                     /locus_tag="KPH29_002867"
     CDS             9096..11081
                     /locus_tag="KPH29_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBG1468449.1"
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSNVLGVSTRTIRNYIN
                     QINRELKGGIAKIVKNEKGTYSIRIEDESKLIEIVNFNRGKLSNFINLNSPDERINYV
                     LDILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGE
                     EMDIRLLILDRLYENYIDVLENVNQINLVKDYDIECLRDELKKLFKKEELYITEQVLR
                     DVERYIIMSVLRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKALLEKIFNIVLNEKET
                     IFLTMPLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLE
                     YHLYFALNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYI
                     ALHFGSYFEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKIGKILGESVEVNTFSDIEAK
                     ANLDLLKYYDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIENAILLKNSINLKSIK
                     KIEKNIPFIGKLIEEDKFFILNKSTFMENLEDMLEKLVHKGIIDIEFKKKVLKREKKS
                     STALDNYIALPHSVNTNGENLYLSFGILEKPVVWDTKEIRIIILMIIPDKNVDSTDLI
                     IKSYEEVLEVGRDKKMIEELAKVKSFDEFYKIITKRR"
     gene            11086..11520
                     /locus_tag="KPH29_002868"
     CDS             11086..11520
                     /locus_tag="KPH29_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HBG1468450.1"
                     /translation="MDPVIFIIASLITVYLLNIFLGYLQLKDFNKNYIELKRKGRVAI
                     GRKKGRISSGTIVLILINDDGVIVETRKMQGVTVLARVKSFEGLVGKSLEYIEKSDLT
                     VYNKLLKKAILDAVKQYITFEKNKCEDTNNLSTDEECRMEVV"
     gene            11549..12109
                     /gene="srlA"
                     /locus_tag="KPH29_002869"
     CDS             11549..12109
                     /gene="srlA"
                     /locus_tag="KPH29_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418408.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBG1468451.1"
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTM
                     NAIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSY
                     FAAASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIK
                     GVVTDYTTKFVEKQQGIALNSDIKIG"
     gene            12173..12541
                     /locus_tag="KPH29_002870"
     CDS             12173..12541
                     /locus_tag="KPH29_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIB"
                     /protein_id="HBG1468452.1"
                     /translation="MAFNKVKIVKGSGGWGGPLIIEPTEKKNKVVYVTGGAKPETAVK
                     IAELTGCELIDGFSQGVKDDEIACVIINCGGTLRCGIYPQKKIPTINIMKTGKSGPLA
                     MFIKEDIYVSGVKPTNVEQL"
     gene            12566..13132
                     /locus_tag="KPH29_002871"
     CDS             12566..13132
                     /locus_tag="KPH29_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBG1468453.1"
                     /translation="MAYDTKKKISEQSQGIVAKIGIALGNVTSTLFQAGRDTIDTLIH
                     TILPFMAFVAVLIGIINQSGIGNWVANLLTPLIGNVGGLVVLSIICSFPLLSPFLGPG
                     AVIAQIVGVLVGTEIGKGNIPPSLALPALFAINSQAACDFIPVGLGLAEAEPDTVQVG
                     VPSVLYSRFLTGAPTVLLAWLASFGLYK"
     gene            13173..13538
                     /locus_tag="KPH29_002872"
     CDS             13173..13538
                     /locus_tag="KPH29_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBG1468454.1"
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHS
                     ENNLVKEILTNDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYL
                     EEKEIAPIGVGTILKIIRK"
     gene            13639..14412
                     /gene="srlD"
                     /locus_tag="KPH29_002873"
     CDS             13639..14412
                     /gene="srlD"
                     /locus_tag="KPH29_002873"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HBG1468455.1"
                     /translation="MRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNAKKVAE
                     EIETEFGCKSVGYGCNAIDERQVISTVKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAG
                     VGLTQSLALDLAEHKITVNALMLGNLLDSDMFESLIPQYAVKLGIPESEVKQVYIDKV
                     PLKRGCRFIDVANMLTFYASKEAEYCTGQSINITGGQVM"
BASE COUNT         5686 a         1754 c         2483 g         4884 t
ORIGIN      
        1 aatggaaact atgtacgtaa taattaaaat aaaaaatatt attttatatt tataaacatt
       61 gacattctaa ttctgtagtg taatcgtatt ttttattaat tcttataaaa tcaatgaaaa
      121 taaggatatc cattttaaag tacataaatt taaaaatcca gaaaaatata cttaaattat
      181 gtaagcaaaa gaggggttga atcggctgaa aatgtaatac tagtaaatag tacatcaatc
      241 ctagataccc tgtaagcaac tctattttca tgtactaaaa atgtacctat tttattaact
      301 gaaaagaata aattaaatgt aagtacataa taagagataa aaaattaaaa gcaaaaaata
      361 tttatataaa taaatataaa aaatgatata atccctatta aaaaatttat agatgatgaa
      421 gaaatatcaa aatcttatat tataggaaga ggcacttctt tgccaaaata tatagaaaag
      481 caataccaaa tccaaagaga ataggctgag aaaaaataaa acaaattcaa gagtaataga
      541 aaagctttat agaataaaga actgagaact ctttatataa taaaaaatgg acttttaaga
      601 gaaaatgatt taataaatga tttaacatgg tgttttagca ggtgaaaata aatcaccagt
      661 aatgattgta gaaaaattac tttatgaatc tcaaaaaaac ttagttaaga gtaagagatt
      721 tgataagata actaaagttg gggaaatgtg tttgatgaga taaaaaaatt agtaaaatag
      781 tataatctaa ataagaatat tttgttgatt aaactacact aatagtaata ttgtaggggg
      841 aaaagatgag agaagaatcg aactctttaa gtttagacca tatagaaatt ttctttgaac
      901 ttttatctca aagtacagaa gattatatat ttttttggga tataaatagg aataaattta
      961 aaatttcttc agcaattttt gatgaattta acttaagtaa agagatagaa tctgatttag
     1021 tcaattgttg gagtaaaatt gtttatccgg atgatgttca aatatggaaa gatgatatac
     1081 aagaattact acatggaaaa aaaggtgagc acaatttaga atataggttg attaataaat
     1141 atgaagaaat agtatggatt tcttgtagag gtaaagttta tgtgtcggat gacccaaaaa
     1201 ctatatttct agtcgggaga ataaagaata ttggtgaaaa aaataaattt gattctatca
     1261 caggaacgtg gaatagagaa caatttgaac atagaatgaa ttatcttata aaagagaaaa
     1321 tttataaaaa tggtgctatg tttatcatgg atattgataa ttttaaaaat ataaatgaaa
     1381 aatatggaca ttcttatggt gacaaagtat tgcgtgcaat tgctactgaa gtattagaat
     1441 atcttccaaa agatgtaaga ttgtataggt tagatggaga tgaatttgca tttttttacc
     1501 ctatgtgtac taaagaaact atagagaaaa tatatgaaaa gatacagatg tatacaaata
     1561 ctcagcatga aattgaatca aataagtact attgcacagt gactgcagga gttgctatgt
     1621 atccagaaga tggagataat tatttagatt tatttaaaca tgctgatatt gcattggata
     1681 tagcaaaaat tagtggtaaa aatagaatta agtttttttc acaagagctc tatgaaaata
     1741 aactaaaggt aatttcaatg caacaaaaac taagagagtg cgttgaaaat aattttaatg
     1801 actttgagct gttttttcaa ccccaggtaa atgctgttac aaaagaggta ataggggcag
     1861 aggtactttt aagatggcat tcgagtactt atggagaagt atctcctgtt gaatttatac
     1921 caatactaga acagagtaat ttaattatac cagttgggaa gtggattata aaagaagctg
     1981 taaaacagtg taaagaatgg cataaaataa atccagactt taaaatatct gtaaatgtat
     2041 cgtatattca gttaaaagaa gattttttta gagattttat agtagaatgt ttagtagagt
     2101 atcaattaag acctgaattt ttaattttgg aattaacaga gaattgttgg ataccagata
     2161 taaatttact aaatgataaa tttattagtt taaagggtat aggagtatat attgcaatag
     2221 atgactttgg tacagggtat tcatctttaa attaccttaa agaactgtct gttaatataa
     2281 taaaaattga aagaagtttt gttaagaata ttacatataa tagttatgaa tatacttttc
     2341 ttgaatatat tattaaatta gctcatatta taaatttaaa agtttgtgtt gagggaatag
     2401 agtcttatga agagtatgat atagtaaaaa gcttaggtgt agatattatt cagggttttt
     2461 tatttggaag acctgtaagt gcctctgaat tttataaatt tcaactatca aagtgatgta
     2521 cataaaatga gatatatagt atataaaaat agggcaaagt ggaagttttg aagtttatta
     2581 aaaatttaca ctttacccta ttttgttatg tcttctataa aggattaata tactacacta
     2641 actattatat actttcactc caaatttaga gattatttca ctagcttttt ggacatcttc
     2701 cttagtggca tcaggaacat cttttagttc ataatcgata cctaaacttt cccatttgtg
     2761 tattcccata gaatggtatg gtagcaattc aaatctttct acatttttca atgtagctac
     2821 aaattgacct agtttttcta aattatctat atcatcagta atgccaggaa ctaaaacatg
     2881 tcttatccat acaggaatat ttcttgattc taagtgtcta gcaagtttta atgccttatc
     2941 cataccaaca ccagtcaaat caatagattt ttcctcaacc atatgtttta aatcaagtaa
     3001 aaccaaatca gtattatcta aaactgggtc tatagtttct atatctacga atccagatgt
     3061 atccaagcaa gtgtgtatat cattttcttt tgctttttta aataattcac ttaaaaattc
     3121 tggttgaaga gtggattcac caccagaggc agttatacct ccaccagaaa acttcataaa
     3181 agaagtatac ttaagagcat ccgttattat ttcatcaact gtgtattctt ttcctgattt
     3241 aatatcccat gtatccctgt tatgacagta tttgcatcta agaggacacc cctgaaaaaa
     3301 taatatatat cttattcctg gtccatctac agtaccaaac gtttctatag aatgtatctt
     3361 tcccttaatc atagagttca cctcacaata tattttaata accttatcaa tagttataat
     3421 atttgtctaa aaaaggccat cttataccag aaataattca aatataagat agccttttat
     3481 ttttattttt tacacagtag taatcttagt taaagtataa aaatttaaag actaagccat
     3541 tttaccgtgg aatgttctgt ttataacatc taattgttgc tctttagtta attttatgaa
     3601 gttaactgca tatccagata ctcttatagt taattgtgga tattcttctg gatgttccat
     3661 agcatcttct aaagtagctc tatcaaatac atttacattt aagtgatgag cattttgaga
     3721 gaagtatcca tccatcattg cagaaagatt gtttattctt tcagtcatat cttttcctaa
     3781 agcagcaggt actatagaga aagtattaga tataccatct tgagcatgtt catatggtaa
     3841 tttagcaact gaagataatg aagctaaagc accattagta tctctaccat gcattgggtt
     3901 agcaccagga gcaaatggag caccagctct tctaccatct ggtgtattac cagttttctt
     3961 accataaaca acatttgaag ttattgtaag tatagattga gttggataag aatttctata
     4021 agttttattt tttcttattt tattcataaa gctttcaact aactctacag ctatactatc
     4081 aactctatca tcattgtttc cgtattttgg ataatctcct tctatttcaa aatcaacagc
     4141 tattccttgt tcatttctaa tagttttaac cttagcatgt tttatagcag ataatgagtc
     4201 tgcacaaact gataacccag ccataccaca agccatagtt ctaaatatat ctctatcatg
     4261 taatgccatt tctaatgctt cataagaata tttatcatgc atataatgta ttacatttaa
     4321 agtatttaca tataatgttg ctaaccaatc agtgaatggt tcaaatttac tcataacttc
     4381 atcataatct aagtactcag aagttatagg ttcaaatctt ggtccaactt gaacaccaga
     4441 tttctcatct acaccaccat ttatagcgta taataaagtt ttagctaagt taactctagc
     4501 tccaaagaac tgcatttgtt taccaattct cattgcagat acacaacaag ctataccata
     4561 gtcatctccc cagtatgctc tcattaaatc atcattttca tactgtactg aacttgtatc
     4621 tatagatact ttagaacaga aatctttgaa tccttgaggt aatttagttg accatagaac
     4681 tgttaagttt ggctctggag aaggtcctaa tgtatataga gtatttaaca ttctaaatga
     4741 gtttttagta actaatgttc taccatctat tcccatacct cctatacact cagttaccca
     4801 agttgggtct ccagagaata ggttattgta atcaggagtt cttaggaact taaccattct
     4861 taatttcata acaaaatggt ccatatactc ttgtaattct tcttctgtta cgattccagc
     4921 ttttaaatct ctttcaaagt atatatctaa gaaagtagat gttctaccta aagacattgc
     4981 agcaccattt tggtctttaa cagcacctaa ataaccaaag tataaccatt gtacagcttc
     5041 ttttgagtta gttgctggtt ttgatatatc aaatccataa gattcagcca tttttttaag
     5101 ttcatttaaa gctgatattt gctcagttat ttcttctctt aatctgataa cttcctcatc
     5161 catgcaagaa acttctaatg atttcttttg ctcattttta tcttctatta aagcatcaac
     5221 accgtataga gcaactcttc tgtagtcacc aattattcta cctctaccat aagcatctgg
     5281 aagacctgtt attataccag attttctagc agctctcatt tctggagtat aaacatcaaa
     5341 aactccttgg ttgtgtgttt ttctgtattt agtaaatata tcttttattt ctgggtctaa
     5401 ttcatatcca aaagcttcgc atgaattttc aaccatcttt ataccaccat atggcatgac
     5461 agctcttttt aaaggggcat ccgtttgtaa cccaactatt gtttctaatt ctttatctaa
     5521 atatcctgga gcatatgcag ctattccaga aacagttttt gtatcaacat ctaaagtacc
     5581 accattttct ctttcttttt taaataggac cattgcttcg tcccataatt ttttagtatt
     5641 ttcagtggca ccagctaaga aagagtcatt tccctcatat ggagaatagt ttaattgtat
     5701 aaactctcta acatttattt ctttagtcca tcttcctgtt ttaaatcctt gccatgcatt
     5761 cattaagtat ttcctcctta gatttaaatg ataaataata ctattaaaat tattttttat
     5821 atacttaatt tattttatat atttttttaa aaatatattt tctaatataa taatatcata
     5881 ttattcgtat cctgtataca taaaaaataa aaaattgtcg ctttaaagta taaaacagat
     5941 tatatgttaa taatgtggtg aaattatacc catatataaa taaaataaaa caaataaata
     6001 gctaattaag gagaaaatat tttttatttt ttgtttagga taaataaatt ttattatgga
     6061 aaaaagaaaa agcatacatc ttcacaaatt gaatatttat ttaatagggg gaggtgatag
     6121 attgtttatc acagtggtat tatttttagt aggtttccta ttaattacta aaggcgcaga
     6181 tatctttata aattgcactg tagagatagg tagaaaaacg aatatatcag aaattatact
     6241 tggagctact atagttagtt ttgcaacaac attacctgaa tttacagtgt ctctacttgc
     6301 ttctattgat ggtcatacta ctatgagttt aggaaatgca gttggttcta taatatgtaa
     6361 tacaggatta gcattagggt tggttgtttt tataagtcca tttaatgtag ataaaaaaat
     6421 gtttttttct aagtctttac tattaatagt atccgttata gttttaattt tattgagttt
     6481 agatggagtt ataacaagag gtgattcatt attacttata ataatattaa tcttttatat
     6541 ggttaataat tatagaagtg tcgttggaaa atctgatact aaaaatagaa atattaaaaa
     6601 taacactatt aaagacatat ctactaaaaa agggaaaaat tatagaggtt tttccgtatt
     6661 agaaattgta aaaatactat tactttttgc tacaggattg ataatgatga taataggttc
     6721 acaaatcctt atagagagtg gagtaataat agctagtttt ttaaatatac ctcagggtat
     6781 agtaagttta actataatag cacttggaac ttctctgcca gagatagttt cgtcgattac
     6841 tgctataaga aaaaatcatc atgaaatatc agtaggaaat attttgggtg caaacatatt
     6901 gaatatagta tcagtaatag ctgtatcagc aataccaaat aacataccaa tactatctca
     6961 aaatagacaa ttggatatac cattcatgat attattatta ttaatagtaa taataccaac
     7021 acttaaatct aatagactta gtagaataca aggtatattg atgttattca catattttct
     7081 gtacatatca attttgtatt tcatgtatat tatttaacta tactgtttaa aatattaatt
     7141 gcagaatatc ctaaaaaagt atataatgga taggttaaga ttaattgata acggagagga
     7201 ataatatatg aatataacaa agtttgaaga tttaccaata agtgagggaa taaaaaaagc
     7261 tatagctgaa atggggtttg aagaaccatc tcctatacaa gcacagtcta tacctgcgat
     7321 tttgtcaggt aaagacgtta taggacaagc tcaaactggt acgggaaaaa cagctgcttt
     7381 tagtatccct atactagaaa caatagaccc aaataataga agtttacaag ctgtagtgct
     7441 ttgtccaaca agagaactgg caatacaagt atcaacagaa ataagaaagt tagctaagta
     7501 ttcgcacgga ataaagacat tacctatata tggtggccag ccaatagata gacaaataaa
     7561 atctctaaag agtggtgtgc aagttgtaat aggtacacca ggacgtacaa tagaccatat
     7621 caatcgtaaa acattaaaga tggataatgt aaaaatgatt atattagatg aagcagatga
     7681 aatgctagat atgggattta gagaagatat agaaatgatt ttaagtaaga ttccagaaga
     7741 aagacaaaca actttctttt cagctactat gccaagagga attttggaat taactaagag
     7801 atatcaaaaa gacccagagc atataaaagt tgttagaaaa gaacttacag tttcaaatac
     7861 aaagcaatac tatatagaaa ctaggtcatc aaataaatta gaagtattat gtagattagt
     7921 agatgtatat gaccctaaac tatctgttgt tttctgtaat actaagagaa aagcagatga
     7981 attggtagga gatttacaag caagaggata ttttgcagat gcattacatg gtgacttaaa
     8041 acagactcaa agagatatag taatggataa gtttagaaat ggtacaatag atatacttgt
     8101 tgctactgat gttgcagcta gaggtataga tgttgatgat gttgaatgtg tattcaacta
     8161 tgacttacca caagatgaag agtattacgt tcatagaatt ggtagaactg gtcgtgctgg
     8221 tagagaaggt atgtcattta cttttgtatt tggaaaagaa atgagaaaga tgaaagatat
     8281 agaaagatat actaaatcta aactaataaa gcataatata ccaacaataa ctgatgttga
     8341 agagaaaaaa gtaggtactt tctttgcaca agttaaacaa actatagaag aaggtcactt
     8401 aactaaacaa ttacaatggt tagaaggttt ctgtaatgat gaagattatg caatggttga
     8461 tatagctgct gcacttgtaa aactatctct aggtgaagaa atgaaagaag aaataataga
     8521 agaaaaacct agaagagagc gtggagatag aaaaggtggt actggagcta aagatggtat
     8581 gattagatta tttatcaata ttggtagaaa tcaaagagta caagcaaaag atatagttgg
     8641 tgctatagct ggagaagttg gaataccagg taaagtagta ggtactatag atatatatga
     8701 taagtatact tttgttgaaa tacctaaaaa agatgcaaaa acagtaattg aaaagatgaa
     8761 agatattaag ataaaaggta ataagattaa tatagaaaaa gcaaataaga agaaaaaata
     8821 acgtatagta tacaaaacta taattagaaa atatataaaa taatatattg gatatttaaa
     8881 aagcctgatg tgttttcaaa acagatagta gataatctgt aagagttaac agcataaggc
     8941 ttttcttgtt ttatatgata ttatgatttt ccagatcgtt gtgaaaaatg tatattagtt
     9001 cctttttttc aaatctgatt tgaaaaagag aatattaatt atctatatag tttagattat
     9061 aataaaaaat gtaagataac aaaggggaga aaaagatgtc aatgacttat atggacccta
     9121 gggttttttt gattttagaa atggtgttag aagaagacac aaatactcta gaacaatttt
     9181 caaatgtttt gggtgttagt actaggacta tcagaaacta tataaaccag attaatcgag
     9241 aattaaaagg tggtattgct aaaatagtta aaaatgaaaa aggtacatat agtataagaa
     9301 ttgaggatga aagtaaatta attgagattg taaattttaa tagaggaaag ctatcaaatt
     9361 ttataaattt aaattcacct gatgaaagaa taaattatgt tcttgatatt ttaactatga
     9421 ccaacaatcc aattaccata gatgatttgg ctgaagagat atgtatagga agaacaacat
     9481 taataaagga tttaaaaaaa gcagacagaa ttttagctga atatgattta gaattaaaaa
     9541 ggaagccaaa tataggtatg attttagttg gtgaagaaat ggatataaga ttattaatcc
     9601 ttgatagatt gtatgaaaat tatattgatg tcttggaaaa cgttaatcag ataaatttag
     9661 ttaaagatta tgatattgaa tgtcttagag atgaattaaa aaagttgttt aaaaaagaag
     9721 aactctacat aacagaacag gtcttgagag atgttgaaag atatataatc atgtctgtct
     9781 taagaaatct aaatgggtac aaatttgaaa agatagataa gagatttgaa gtgataagat
     9841 gttcagatga atatacttta ggattaaagc tcaaagcttt attggaaaag atatttaata
     9901 tagttttaaa tgaaaaagaa accatatttt tgacaatgcc attaataggt agaaaagctc
     9961 ctagtacaaa attagcactt agcagtataa agataaatga tagcgtaaag gaagttgtag
    10021 atgaagtaac tagtttttta ttagagacct cagggattga ttttaaagaa gataaaaagc
    10081 ttatagaaaa tttggaatat cacttgtatt ttgcacttaa tagaatgaga ttcaacataa
    10141 gggtaaaaaa tcctctttta caagaaataa aggaaagata tacatttcca tatagtgttg
    10201 caaagatagc tgcaatgata attgaggaaa agtttgactt gaaaatatgt gaccacgaaa
    10261 taggatatat agcattacat tttggaagtt attttgaaaa aaattcaaga aaagttctaa
    10321 atgtaaatag ggtagctgtt gtttgtggaa caggtcttgg gactgcacaa cttattagaa
    10381 taaagatagg aaaaatactt ggagaaagtg tagaagtaaa tactttttct gatatagaag
    10441 ctaaagctaa tttagattta ttaaagtatt atgatttagt atttacaaca gtagatttca
    10501 aaacagactt agatgttcca atttttagag taaatgcgtt atttaatgaa gaaagtataa
    10561 agaaagagat agaaaatgca atacttttga aaaatagtat aaatcttaag tctataaaaa
    10621 agattgaaaa aaatatccct tttataggta aattaattga agaggataag ttctttatat
    10681 tgaataagag tacgtttatg gaaaatttag aagatatgtt agaaaaatta gttcataaag
    10741 gaattattga tatagagttc aaaaaaaagg tattaaaaag agaaaagaaa tcttcaacag
    10801 cattagataa ttatatagca ttgcctcact cagttaatac taatggggaa aatctttatc
    10861 tgtcatttgg aatattagaa aaaccagtgg tttgggatac aaaggaaatt agaataataa
    10921 tattgatgat aattccagat aagaatgtag atagtacaga cctgataata aaaagttatg
    10981 aagaagtact tgaagtaggg agagataaaa aaatgataga agaacttgct aaagtaaaat
    11041 catttgatga attttataaa ataattacca agaggaggta ataatatgga cccagtaatt
    11101 tttattatag catcacttat tacagtgtat ttattaaata tttttcttgg atacttacag
    11161 cttaaagatt ttaataaaaa ttatattgaa ctaaaaagaa aaggaagagt ggcaataggc
    11221 agaaaaaaag gaagaatttc atcaggtaca attgtgttga tacttataaa tgatgatggt
    11281 gtgattgtcg aaactagaaa aatgcaaggt gttacagtat tggcaagagt aaagtcattt
    11341 gaaggtttgg taggaaagag cttagagtac atagaaaaat cagatttaac tgtttacaat
    11401 aaactactta aaaaagcaat actagatgca gtcaaacagt acataacatt tgagaaaaat
    11461 aaatgtgaag atacaaataa tctgagtact gatgaagagt gtaggatgga agtagtttaa
    11521 aaaaatatat ttataaggag gagttaatat gacgcaattt tttcaagtat tagcaagtgg
    11581 tgcagatagt tttatgaatt tatttagggc aggtgggcaa caatttgtag gttttgtaac
    11641 agatatagta ccacttttag tagcattatt agttacaatg aatgcaatta tcaactttat
    11701 tggtacagat agagtcgaga gacttgcaaa aaaatgttct gcaaatatat ttactagata
    11761 tttgatatta ccagtccttg gtacatttgt atttgcaaac ccaatgacac tatcactagg
    11821 aagattcttg ccagaaaagt ttaaacctag ttactttgca gcagcaagct tttcatgcca
    11881 tacaatgaat ggattattcc cacatatcaa tccaggtgaa ctatttatat atctaggaat
    11941 tgcaaatggt ataacaactt tgggattctc aacagcagat ttagcagtta gatatttgtt
    12001 agtgggtata gttgcaaact ttattaaagg tgtagttact gactatacaa caaaatttgt
    12061 agagaaacaa caaggaatag cattaaattc agatataaag attggataat tactttaagg
    12121 tataaataaa tttttcaaat aaaaagatag tgtatagagg aggataaatt atatggcatt
    12181 taacaaagtt aaaatagtaa aaggttctgg aggatggggt ggaccattga taatagaacc
    12241 aacagaaaag aagaataagg tagtatatgt gactggggga gcaaagccag aaactgcagt
    12301 aaaaatagct gaacttacag gatgtgagtt aatagatgga ttttcacagg gtgttaaaga
    12361 tgatgaaatc gcatgtgtaa taataaattg tggtggaaca ttgagatgtg gtatatatcc
    12421 tcaaaagaaa ataccgacta taaatataat gaaaacagga aaaagtggac cattagcaat
    12481 gtttataaaa gaagatatat atgtttcagg ggtaaaacca acaaatgtag agcagttata
    12541 attgaggtag taggagggaa gaaatatggc ttatgatact aaaaagaaga taagtgagca
    12601 gagtcaagga attgttgcaa aaataggtat agcactagga aatgtgacaa gtactctttt
    12661 tcaagctggt agggatacta tagatacatt aatacatact atactgcctt ttatggcttt
    12721 cgttgcagtc ctaataggta taataaatca atcaggtata ggaaattggg tagccaattt
    12781 acttactcca ttaatcggaa atgttggagg tttagttgtc ttatcaataa tatgttcatt
    12841 tccgttgttg tctccgtttc ttggaccagg agctgttata gctcagatag tgggggtatt
    12901 ggttggtaca gaaataggaa aaggaaatat tccaccaagt ttagcattac cagcattgtt
    12961 tgcaataaac tcacaagcag cttgtgactt tataccagta ggtcttggtc ttgcagaagc
    13021 tgaacctgat acagttcaag taggagttcc gtcagtttta tattctagat ttttaacagg
    13081 tgcaccaact gttctattag catggttagc aagctttggg ttatataaat agaagaatac
    13141 tttataaata attagagaga ggttcagttg atatggaatt aatatacagt actcaaataa
    13201 ataaaatagg agataatgca aatgaatttt tacaacataa tatgtttgta actttcaaag
    13261 ataatgctcc tcaagagtta gaaaattact gttatataca ttctgaaaat aatttagtaa
    13321 aagaaatact tacgaatgat gtgttatcaa taaataatgt tgattacaaa attacttctg
    13381 ttggtgagtt agtaaaccaa aatctttcag aattaggtca tataacattt aagtttacag
    13441 gtgaaaaaga tggagcaatt ggagggacct tatatcttga agaaaaagaa atagcaccta
    13501 ttggcgtagg tactatacta aaaataatta gaaaataaaa tttaaaacga atctaatcat
    13561 attataagta aattatagat aaaagtcaat ttaactaatc aagttaactt ttaatgtgat
    13621 aaaaaagggg aaatgaaaat gagaaaagtg gctttagttg ttggaggagg aagaagttta
    13681 ggtgaatatt tatctaaaca tttagctaaa gtaggttatg atgttgctat tgcagattta
    13741 aatgaagaca acgcaaaaaa agtagctgaa gaaatagaaa ctgaatttgg ttgtaaatct
    13801 gtaggatatg gttgtaatgc catagatgaa agacaggtta taagcactgt taaagctatt
    13861 gaaaatgatt ttgatagaat agatttatta gtgtacaatg caggagtggc tgtaagtaaa
    13921 aaaataactg attttggata caaagatttt aaatttattg tagacgtaaa tttaaatggg
    13981 tactttctat ttgcaagaga agtatctaaa gtcatgatag caaaaggtat tgagggtaga
    14041 atcatacaga taaattcaaa atcaggtaga gtgggaagca agcataattc aggatattca
    14101 gcaggtaaat ttgctggagt aggacttact caaagtttag cgctagattt ggcagaacat
    14161 aagataactg taaatgcact gatgctcggt aatttacttg attctgatat gtttgaaagt
    14221 ttaattcctc aatatgcagt aaaattggga atacctgaat cagaagtcaa gcaagtttat
    14281 atagataaag tacctttaaa aagagggtgt agatttatag atgttgcaaa tatgcttaca
    14341 ttttatgctt caaaggaagc tgaatactgt actggtcaat ctataaatat tactggtgga
    14401 caagttatgt aaggttttta tggagtgtct caagatgaac tctttagttc atgaggcact
    14461 ttttttgtat aaaattaaat atattttcat catttaaaca atgaaaaaga ggtttatctt
    14521 tattttgaga tagcctcatt tttgtaatta agaaacatag tgtttaagta aacatataag
    14581 attgttagtt ttttaaaaga gaatttttta ataatattat atttatgaag tatttttaaa
    14641 cttaatttga ttaaaaaata gctacgcaca cttgtgactt tagtcatgag ttagtagcta
    14701 aattagtcag catataggga aacttatatg tagagatagg atagaaccta tccaatacta
    14761 cttgaattgc taaaaacccc taaagctaac caaactaaaa cataagg
//
LOCUS       DAELXX010000100        14464 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.100, whole genome shotgun sequence.
ACCESSION   DAELXX010000100 DAELXX010000000
VERSION     DAELXX010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14464
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.100"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            270..1283
                     /gene="aroF"
                     /locus_tag="KPH29_002874"
     CDS             270..1283
                     /gene="aroF"
                     /locus_tag="KPH29_002874"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBG1468456.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKIVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            1305..2369
                     /locus_tag="KPH29_002875"
     CDS             1305..2369
                     /locus_tag="KPH29_002875"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBG1468457.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFNKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            2369..3682
                     /gene="aroA"
                     /locus_tag="KPH29_002876"
     CDS             2369..3682
                     /gene="aroA"
                     /locus_tag="KPH29_002876"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBG1468458.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLAFDGVKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNSTLIDASQCPDIIPVLSVVASLSIGRTTIIN
                     AGRLRIKECDRLHAINVELSKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            3660..4739
                     /gene="aroC"
                     /locus_tag="KPH29_002877"
     CDS             3660..4739
                     /gene="aroC"
                     /locus_tag="KPH29_002877"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBG1468459.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDKIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQRDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            4742..5938
                     /gene="pheA"
                     /locus_tag="KPH29_002878"
     CDS             4742..5938
                     /gene="pheA"
                     /locus_tag="KPH29_002878"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBG1468460.1"
                     /translation="MEDLTRYRNEIDNIDKEIIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAIDNLDNKAYSQETVQFFNSIMEISRGLQKRLIDNDGEQKIEFSLDS
                     SNKKFDLSNVNKYKSLKDELNKKNILVGFPGKSGSFTEEALNKFFNKTISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSEFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYELEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAENNINMTKIESRPMKNASWRYFFYIDFECSIYNSKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            5985..6794
                     /gene="aroE"
                     /locus_tag="KPH29_002879"
     CDS             5985..6794
                     /gene="aroE"
                     /locus_tag="KPH29_002879"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBG1468461.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRIFEILNIESAYKNFEIPKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            6806..7339
                     /locus_tag="KPH29_002880"
     CDS             6806..7339
                     /locus_tag="KPH29_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBG1468462.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDVNMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAVY
                     NITNAVSENFKFDFKEK"
     gene            7360..8199
                     /locus_tag="KPH29_002881"
     CDS             7360..8199
                     /locus_tag="KPH29_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBG1468463.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNNDKNKLIEYFKKSSIRREA
                     LEK"
     gene            8467..9948
                     /locus_tag="KPH29_002882"
     CDS             8467..9948
                     /locus_tag="KPH29_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG1468464.1"
                     /translation="MKKNRLIIKNISIAFIVLFFFSVFTFFYIGNISRVLEYETNDIM
                     TVTITGWIILSFLFLCIIIYILYSKAKSQKTIEKVAYTDFVTGYSNWRKFELDVTNLL
                     KKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLNELTNINETFARVSAD
                     NFNILLTYNKKEDIINIIKKIIANNELVNLSFGIYEIKDKDLSVSVYSDRASLAKSSI
                     KNNSDVNFAFFNDKLREKLLLEDKIEKEMEYALESGQFVMYLQPKYNIKLDKFCGSEA
                     LVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIRSLFDKGISPLPISVN
                     FSRVDFLKKDFIENIVNICDRYKIPYSLIEIEITESSMFGDTDTLFNVSRNLQDIGFI
                     VAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVDDKDVDKSQIVIKSIVSLIKQLGIRV
                     VAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            9981..13418
                     /locus_tag="KPH29_002883"
     CDS             9981..13418
                     /locus_tag="KPH29_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBG1468465.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSKYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPIFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIMLIIYIIYKNNKSQKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEISKQL
                     YKNKTIELEYRIITKNGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENILGGIIITEFNDDFTIKYTNSGYLSMIGYTREQLENELKYKACNLVFY
                     EDRKKALESIQHQLNTKGIAVYQHRIRRRDGNIIWVSIKGRLIIDDDGIEKGIWILND
                     ITDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITKNV
                     PYSLVESGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDE
                     DGNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNESW
                     QKPFGIKPDNNYSEMVKTISNSIIHKDNKEVFLNTFLCKNLLSAYNKNITEIKLEYRR
                     MNQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIKLKLKAERDTLTGLYN
                     KGTTENLINDFLNSDEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKHLKPI
                     FRKDDIVGRIGGDEFIVFLKNIQSFENTKKKAEKICDIFRNSYTENKKTYKISGTVGI
                     SFVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNNCIYQNELENNISDNNIKLANK
                     CEKSKPVLKEILDSIDNYIYIVNPNTYELLFVNNKVSRLDLEIKLGETCYKSLLKSER
                     PCNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            13436..14182
                     /locus_tag="KPH29_002884"
     CDS             13436..14182
                     /locus_tag="KPH29_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBG1468466.1"
                     /translation="MLNSITLILGIIFTIYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIIISIFLIVFVVTESLIIFYPKNSLESKSDYLLILGASVKK
                     TTPSTTLKGRLDTALKYLKINDNCYVVVSGGKGSGEKITEAKAMKDYLIKNSIDKNRI
                     IEEDKSTNTYENFKYSKLKIEEHSQRKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIIFDR"
     gene            complement(14131..14451)
                     /locus_tag="KPH29_002885"
     CDS             complement(14131..14451)
                     /locus_tag="KPH29_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBG1468467.1"
                     /translation="MEKIMNFLKENSTFYFATVEDNKPRVRSFGFIMDYEGKLYFGIV
                     KYKTSYKQLLVNPNVEISTTSKVGKWIRIKGIEKTRYEFISSLFIYYLSKMIVFHNAK
                     NSLV"
BASE COUNT         5984 a         1445 c         2408 g         4627 t
ORIGIN      
        1 aagaactaac agcaaaactt tatagctttt tttactttat gttctggggc ttttatttta
       61 gcgctggcta tttcttttac aaaaatatat gtacttcctt tctggtgagg ccttatttta
      121 gtgattttga gcataagtaa attttataaa aatgattatt ttatatattt atataagatt
      181 tagtgcttga tagattattt tagagaactc attagtgagt tctctttttt attttaaata
      241 taaaattata atattgggag gtagtaaaaa tgatagtagt attaaaaatg ggagcagata
      301 aaaatgaagt taaaaaatta attgaggcta ttgggagaga aggagtagaa gttaatccca
      361 tagatggaac agaattaact gttttaggtt tagttggaga tactagtaaa atagatgcca
      421 aaagaataga agcaaataag atagttgaaa aagttatgca tgtagtagag ccttttaaaa
      481 aggccaatag aaaatttcat ccagaacctt caatcataaa tgttaatgga atggaaattg
      541 gttcaaagaa aattgctatg atagctgggc cttgttctgt tgaaacagaa gaccaaatag
      601 tatctatagc taaagatgtg aaaaaatcag gtgcaggatt tttgagaggt ggagcattca
      661 aaccaagaac atcaccatac gctttccaag gattgaaata tgatggattg gatttattaa
      721 aaaaagcaaa agaaaaaaca gggcttccta tagttacaga aataatgtca acacaagata
      781 tagatatatt tgaagaaaat gtagatgtaa ttcaagttgg agctagaaac atgcagaact
      841 ttgatttatt aaaagaatta ggtaaaacaa ataaaacaat acttttaaaa agaggattat
      901 ctgctacaat tgaagagtgg ctaatgtcag cagagtatat aatggctgga ggaaatgaaa
      961 atgtagttct ttgtgaaaga ggtataagaa cttttgaaac atatactaga aatactttgg
     1021 atttaagtgc aattttagct gtgaagaaac ttagtcattt accagttata gttgacccaa
     1081 gtcatgcagc aggtaaatca tggatggttg actctttatc taaggcagca attgcagttg
     1141 gagcagatgg tctaataata gaagttcata atgacccagc acatgcactt tgtgatggaa
     1201 aacaatcgat taagccaaat gaatatgatg aattgataag tgaattaaag actatcgcat
     1261 cagcagttgg tagagaaata taatctataa aaggatggtt aaacgtggaa gaaaaactat
     1321 tagtaaattt aaaagaaaat agctacaata tatttattaa aaaaaatata ctaaagaata
     1381 taggagaaga aataaaaaaa atatattttg gaaataagat ttttattgta actgatgaaa
     1441 atatagcaaa attatacttg gataaagtaa aaaataattt aagtgaatct ggatttgaaa
     1501 ttaaatcttt aatattaaaa tctggtgaag aaacaaaatc atttaatact cttcctaaaa
     1561 tatacgatag tttacttgac tttaagttaa ctagaaaaga tttgattatt actttaggag
     1621 gaggggtaat aggtgattta ggtggatttg cagcagcaac ttttttaaga ggtataagtt
     1681 ttattcaaat tccaacatct ttattagcac aagttgatag ttctgtaggt ggaaaagttg
     1741 gagttgacct tgagagaggc aaaaatttag tgggaagctt ttaccaacca aaagctgtgt
     1801 ttatcgaccc aaatgtatta ttatcacttc cagaaaaatt ctatagagat ggaatggcag
     1861 aagttataaa atatggatgt ataaaagaca aaactttttt caataaactt ttaagtttaa
     1921 aaaatagaga agaagtaatg caacatatag aatacattat acataaatgt tgctttatta
     1981 aaaaaactgt agttgaagct gatgaaaaag actttggaga tagaatgctt ctaaactttg
     2041 gccatacatt aggtcatgct attgagaaat actacaattt tacgggttat acccatggag
     2101 aagctgttgc cataggaatg tataagataa ctttagaaag tgaaaagata ggaattacaa
     2161 aaaaaggtac atcagaactt ataaaatcaa ttttaatgaa ttataatttg ccttatgaga
     2221 ttgaattaag agataataaa tctatagtag atgctatatc tcttgataaa aaaaatctag
     2281 gtgatacatt aaaaataata cttttaaaag atattggaga gagtattata tataatacta
     2341 cttctaaatt ttttcaggag gtaagataat gggaagtcta aaaatatatc caagtaaatt
     2401 atctggagat gtaaaaatac caccatcaaa aagtatggca catagggcag ttatttgttc
     2461 atctctaagc aatggaaaaa gtagaatttc taatatagat ttttctgatg atataatagc
     2521 tactataaga gcaatgacat cattaggagc tattatagaa aagaaagaag atattttaga
     2581 aatttcggga attttttcta aagaaggtat tttaaataga gagaatcagt tgaatcaacc
     2641 taaattgaca atagattgta atgaatcagg ctctacatta agatttttag tacctatatc
     2701 tttagcattt gatggtgtaa aaaggtttat aggtagaggt aatcttggaa aaagaccact
     2761 tgatacttat tatgagatat ttgacagaca gaatataaaa tatagttaca aagaaaatca
     2821 gttagatttg attatatcag gtaagcttaa accagatgaa tttagagtaa aaggaaatat
     2881 aagttctcag tttatcacag gtctattgtt tatattgcct acactagaga gtgattcaaa
     2941 gataataatt actactgagc tagaatctaa aggatattta gatttaactc tttcaacgat
     3001 aaaagatttt ggggtagaaa ttataaacaa taactataaa gaatttatta taaaaggaaa
     3061 tcaaacgtat aaagcgagag attataaagt agaaggagat tattctcaag gagcatttta
     3121 tttatcagca gatgctatag gagaagatat tagcatatta gaccttaaag aagattctct
     3181 tcaaggggat agtgaagtag ttgaaatttt atctaggatg ggaatggaaa tattaagaga
     3241 aggcaacaaa ataaaaggaa taacaaatgg tttaaatagt actttgatag atgcatctca
     3301 atgcccagat ataatacctg tattatctgt agtagcatcc ttaagtatag ggaggactac
     3361 aataattaat gctggtaggc ttagaattaa agaatgtgat agacttcatg caataaatgt
     3421 tgagttaagt aaacttggag caaatataga ggagaaagag gatagtttga taatcgaagg
     3481 agtaagcaag ttaaatggtg gagtagaggt gtggagtcac aaagaccata gaattgctat
     3541 gacacttgca attgcttctt gtagatgtga taaaccaata atattaaaag attttgagtg
     3601 tgtatcaaaa tcttatccac atttttttaa agattttaag atgttaggag gtcgaattga
     3661 tgagtggaat atggggaaat aatttaaaag tatcaatttt tggagaatct catggaaatg
     3721 ctataggtat aaatatagat ggtcttcctt cggggattga acttgacctt gataagattg
     3781 ataaagaaat gaagagaaga gcacctggaa aaaattctat atctacttca agaaatgaaa
     3841 gtgatatacc agaaattcta agtggatatt ttaatggaag aactacagga acgccattat
     3901 gtgcaatcat aagaaatagt gatactcgtt caaaagatta tggagagctt aaaaatttaa
     3961 tgagaccagg acatgcagat ttcacaggta atgtgagata ttctggattt aatgactata
     4021 gagggggagg tcatttttca ggtaggataa cagctccatt agtattttgt ggggctatat
     4081 gtaaacaaat tttatcacaa aaaggaatag agataggagc acacataaaa aaaattaaaa
     4141 atatagaaga tatgagtttt gattatgtaa atatttcaaa acaacagtta tcaaatttgc
     4201 aaactttgga actaccttta cttgatttat caaaggaaga agccatgaaa aatacgatta
     4261 tagatgcaaa aaatcaaaga gattcagttg gtggtataat tgagtgtgca gtagtaggta
     4321 ttaatgtagg gcttggaaat ccattttttg actctgttga atctacttta tcacatttat
     4381 tgttttctgt tcctgccgta aaaggtgtag agtttggttt aggttttgaa cttgcagata
     4441 tgtatggctc tcaaagtaat gatgaaatgt attatgaagg aaatcaagtt aagagcaaaa
     4501 caaataacaa tggagggatt ataggaggga ttactactgg aatgccaata attttcaaag
     4561 tggcaattaa gccaactcct tcaattagca gacaacaaaa cacagttaat ataaaagata
     4621 aaaaggacga tattttatat attaaaggca gacatgaccc ttgtatagtt caaagggcta
     4681 tacctgtaat agaagcagta acagccattg gaatttttga tttgatgaaa ggacgataat
     4741 aatggaggat ttaacgagat atagaaatga gattgacaat atagataaag agattattca
     4801 attatttgaa aagagaatga atacagttct tgaaattgcc agacataaaa tgaaaaataa
     4861 cactactata cttcaaaaag atagagaaga aaaagtacta agtagagcca tagataattt
     4921 agataataaa gcatactcac aagaaacggt acagtttttt aattctataa tggaaattag
     4981 tagaggatta caaaaaagat tgatagataa tgatggtgag cagaaaatcg agtttagttt
     5041 agattcatca aataagaaat ttgatttgtc aaatgtaaat aaatataagt ctttaaaaga
     5101 tgaattaaat aaaaaaaata tcctagtagg atttccagga aaatcaggtt catttacaga
     5161 agaagcacta aataaattct tcaacaaaac aatatcaaaa aaacaattta aagaatttga
     5221 agatgttttc atagcactta aaaataaaga aataaactat ggaataatcc ccatagaaaa
     5281 ttcatctaca ggagctatct ctgaaactta tgaccttttg agaaagtacg gtttttatat
     5341 agttggagaa gaatgtataa aaatagacca aaatttaata gggataaaag acacaaaact
     5401 tgaagatata aaagaaatat attctcatcc acaaggtata ggacaatgta gtgaattttt
     5461 aaaacaaaat agtacatgga aattgatacc atttaataac acagcaacaa gtgcagaact
     5521 tgtaagaaaa cttcaagata aaacaaaagc agcaattgca agcaaaaaag ctgcaagtat
     5581 ttatgaatta gaaataataa gtccttgtat aaatgatatt gcaaacaact atacaagatt
     5641 tgtagtaatc agtaatcaaa tacacattga agaagaatca aacaaaatga gtgtagtatt
     5701 ttctgttgaa catgaagcag gaaaacttta taaagtactt ggatactttg ctgaaaataa
     5761 tataaatatg acaaaaatag agtcaagacc tatgaaaaat gcctcatgga gatatttctt
     5821 ttatatagat tttgagtgtt ctatatacaa ttctaaagtg tacgacttat tagaattgat
     5881 agaacataat acagcatatt ttaaatttat gggagtatat aaaaataaaa ctgaatagat
     5941 aatataaatt tttatttaat ttataattat taggaggtgt aagtatgaat tttttcggtc
     6001 tagtaggtga aaaactctct cacagtgttt cacctcaaat ccacaagaga atattcgaga
     6061 tattgaatat tgaaagtgct tataaaaact ttgaaattcc aaaagaagat atatctaagc
     6121 tagacggtgc tataaaatta ttaggaatac aaggagtaaa tgttactgta ccatataagg
     6181 agcgtattat gaaatatctt gattttatat caccagaggc aaaacgtata ggagcagtta
     6241 atacaatttt actcagagaa aatatgttat atggttacaa tacagattac tttggtcttg
     6301 atagtatgtt taaaatggct aatattgatg ttcaaggaaa agtagcagtt atattaggaa
     6361 ctggaggtgc ttctaaagct gctcttacat attttattga ttctggtata gaaaagcttt
     6421 atgtttcaac tagaaaaaag gatgataaaa aattattaaa tagtaaagct atattaatag
     6481 attatgaaga acttaaacat ataaaaggag atataatatt aaatgctaca ccagtaggta
     6541 tgtaccctaa tgtaggaatt tcaccagtgt caaaatctat tatacaaaat tttgatatat
     6601 taatagattt aatttacaat cctggagaaa cagagttttt aagaattggt aatagtatgg
     6661 gtaaaaaaac ttgtgatgga ttatatatgc tggtagggca agcaataaaa tctcaggaaa
     6721 tttggcaaga tactaaaata gataatagta ttttagatgt tatatataat gaactaaaat
     6781 tagaattttt gtaagggaga ttgtcatgat aaataaaaca aaagaaaaat tgatattaat
     6841 aggtatgcca ggtagtggaa aaactacaat tggtaaactt ttggccaaag aatataattg
     6901 tagtttctgt gatatggatg attatataat tcaaatatct caaaaaagta tagcagagtt
     6961 gttttcagaa ggagaagata tctttaggaa ctatgagact caagcatgta gagaactttc
     7021 tataagtgat aaaacagtga tatctacagg tggaggtgta ataaaaaagg atgttaatat
     7081 ggaaatacta aaagaaacag gtataataat ttttattgat agacctatac aaaagatttt
     7141 ggaagatata aatattaatt caagaccatt attaaaaaac ggaaaagata gattgtataa
     7201 tctttataat gagagaataa atctttataa aaaattttca gatatagaga ttttaaatga
     7261 taaatcatta aataatgctg tatataatat tacaaatgca gtcagtgaaa attttaaatt
     7321 tgactttaaa gaaaaataat ctagggggaa ataaaaataa tgaatattgt tattgtagga
     7381 ctaggagtta taggaggctc ttttgctaaa gcattaaaaa aagctggata tgaaaatgta
     7441 tttggagtag atgtagattt agaaacttta aaaaaagcag aaaaagcaaa aatcataaaa
     7501 aaaggatgta ctacaggtaa agagttattt aaaaaagctg atttaattat actatctata
     7561 tatcctagat tagttgtaga ttttctaaat aataataaaa acttttttaa aaaagggact
     7621 ataattactg atactacagg aataaaagaa acacttatca atgatgtgtt gcaaattatt
     7681 ccagatgata tagattttat atttggacat ccaatggcag gtagggaaaa aaagggtatt
     7741 gattttgcaa gtgaacaagt ttttaatggc gcaaattata taattactcc aactggcaga
     7801 aacaacataa aaaatttaga gttagttgaa aatttaattt tagagattgg atttaaaaga
     7861 gtaaaaaagt taacttcaca aaaacatgat gagattatag cttttacttc tcagttacct
     7921 catgttatgg cagttgctct tataaatagt gatgaagaag gtagagatac aggtaaattt
     7981 attggagata gttatagaga tttaactaga attgcaaata tgaatgaaga cttatggagt
     8041 gaactttttt taggaaatag agataattta ctaaaagtta tagaaaactt tgaatcagaa
     8101 gtaaatttag taaaagaagc tatatttaac aatgataaaa ataaattgat agagtatttt
     8161 aaaaaatcat caataagaag agaagcttta gaaaaataaa tattaatatt taagctatat
     8221 atagatgggt ataaatgtaa gcgatagtgt atttaattgt attaataaat atggattcaa
     8281 tataattaaa tatgctattg atatatttgt acccttaact attgttatct tttaaatgat
     8341 aatgatattt atttaaatgc tctttattaa caatttataa aacttaattg taaaaaatac
     8401 ttattaatag taaaataaag tgaatcaatt atattataat tgctaaagag agtgaggtgt
     8461 agctatttga aaaaaaatag attaataata aagaatatat caatagcatt tatagtacta
     8521 ttctttttta gtgtatttac attcttttat ataggtaata ttagtagagt attagaatat
     8581 gaaacaaatg acataatgac agttacaatt actggatgga ttatactttc atttttattt
     8641 ttatgtatta taatttacat attatattca aaagcaaaga gtcaaaaaac tatagaaaaa
     8701 gtagcatata cggattttgt aacaggttat tccaactgga gaaagtttga attagatgtt
     8761 acaaatttat taaagaaaac atcacaaaat aataaatatg caatggtcat attcgatatt
     8821 gataaattta aagcaattaa tgatatatat ggacataaaa agggcaattt aatattaaaa
     8881 gatattgcag atactttaaa tgaattgact aatatcaatg aaacttttgc tcgtgtaagt
     8941 gctgataatt ttaatatatt attaacatat aacaaaaaag aggatataat aaatattatt
     9001 aaaaaaatta tagcaaataa tgaacttgta aacttatctt ttggaattta tgaaatcaaa
     9061 gataaggatt tatctgtaag tgtatatagt gatagagctt cacttgcaaa atcatcaata
     9121 aaaaataata gtgatgtaaa ttttgcattt tttaatgata aattaagaga gaaattatta
     9181 cttgaagata agatagaaaa agaaatggaa tatgctcttg aaagtgggca attcgtaatg
     9241 tatttacaac caaaatataa tattaaatta gataaatttt gtggaagtga agccctagtc
     9301 agatggcaat atacagaaaa agaagtcata tatccaggtg attttattcc tatatttgaa
     9361 aaaaatggat ttattagaaa aattgatatg tacatattag aacaagcttg taaagaaatc
     9421 cggtctttat ttgataaagg aatttcacct ttacctatat ctgtaaactt ctcaagagtt
     9481 gattttttaa aaaaagattt tattgaaaac atagtaaata tatgtgatag atataaaata
     9541 ccatatagtt taatagaaat tgaaattact gaatctagta tgtttggaga tactgatact
     9601 ctatttaatg ttagtagaaa cctacaggat attggtttta tagttgcaat ggatgatttt
     9661 ggctcgggat attcttctgt aaatatgctt aaaaatatac cattaaatgt aataaaatta
     9721 gatagaggat tttttgttga tgataaagat gttgataaga gtcaaattgt tataaaaagc
     9781 atagtttctc tcattaagca attaggaata agggttgttg ctgaaggaat tgaaacaaga
     9841 agtcaaatag aaatgttaaa aaaagcaaat tgtgatattg ttcaaggata ttatttttca
     9901 aaaccattac caattaaaga atttgaaaaa ttagtctata aaatatagtc agcgagtctt
     9961 aaatgggagg tggtgattaa gtgataagag caaaatcgtt aaaaaatgta ctgagtattg
    10021 ttttaatatt tttatttaca ataatttctt tttctgattt tgcatttaaa actaatgtcc
    10081 aggaagaaga aaatagtaaa attaatttaa gggaaatatc cctacaaagt tccaatgcta
    10141 taaaaataaa attagatagt tctctatcct ttattaaaag tgtggcaaat atggtttctt
    10201 attttaatac tatagataac tcaaaaatag ttccattatt agctaacata tcaaaagaat
    10261 ctgactttca aagaatctct attgtatctc ctgatggaat aagtcatacc agcgatggca
    10321 aaattatcaa tgtgtcaaaa tatcagtatt ttaagaaagc tattaaagga gaaagcaata
    10381 tatctgaagt tgtgaaatct ccaattgatg gaaaagatac ttttgttata gctactccta
    10441 tatttaataa gaataacaaa ataattggtg ttttatacgg ttcttactac aatgataaat
    10501 tagctgaatt tatagatata acaagtttta atagtgaagg gtacatagat atatttgaat
    10561 ctagtggtca atttgtttta aaatcttcac atacaaacaa actgattaag tcaagtagta
    10621 acatatatga tatatttaaa aaagcttctt ttgatgatgg ttatagctat aaaaaaatat
    10681 atgaagatac aaaaaataac aagagtggct ttacctcata tgaatacaaa ggagaaaatc
    10741 gttatgctac ttatacaccc attggtgtaa atgactggta tgtaatttct gtgataccac
    10801 aatatactgt aagaagaaac attgataaaa taaatcaatt agcattttct cttattttca
    10861 aagtattctt attgtttatt atgcttataa tatatattat ttataaaaac aataaatctc
    10921 agaaagatat actaaaagct aacaaagaaa tctcatctat tacaaatagc attcctggag
    10981 gagtacaaaa gtgtaccctg ggtgaaacat gcaatattaa ttttttaagt gatggattta
    11041 tagagttgtt tggttataca agagaagaaa taaaaacatt gtttaataat gatttttata
    11101 gtttaataca taaagatgat gttgaaagaa taaaaaaaga aatttctaaa caactttaca
    11161 aaaataaaac tattgaatta gaatatagga ttataacaaa aaatggtaat acagtatggg
    11221 ttttagataa aggagaactt ataacagaac ctgatggaaa agaatatttt tgttgtgtaa
    11281 ctgtagatat tactagaaca aaacaattag aacaagagat ggaacaaaaa aatctagaac
    11341 ttgaaatgat atcagaaaat attttaggtg gtataattat aacagaattt aatgatgatt
    11401 ttactataaa atacactaac tctggttatc tttcaatgat tggatataca cgtgaacaat
    11461 tagaaaatga acttaaatac aaagcttgta atcttgtttt ttatgaagat agaaagaagg
    11521 ctcttgaaag tatacaacat caattaaaca caaaaggaat agctgtatat caacatagaa
    11581 taagaagaag agatggtaat attatctggg tttctataaa aggaaggctt attattgatg
    11641 atgatggtat tgaaaaaggt atttggattc taaatgacat aacagattta aaaaatgcag
    11701 aacaaaaact taaaataaat gaagaaagat ttagaattgc attaaagcaa acatcaaata
    11761 ctatatttga gtatgatata aaaacaaagt cacttttaaa tactagtaaa gagcttgata
    11821 aatataatat ttataaaatt actaaaaacg ttccatattc tctagtagaa tcaggagtta
    11881 ttcataaaga ttttagtgat gattttataa acatgtatga aaatgtaata aatggagcaa
    11941 aaaaagcaag ttgtgtagtt aagatgaaac ttccagatga aaattatata tggaataaag
    12001 ttaccttgac tactattttt gatgaagatg gtaatcctgt aagagcaatt ggtatacagg
    12061 aagatataag caaacaaaag gaagcagaga taagatataa tcaagaaggt aagtatcatt
    12121 cagctatatt gtcggaagca atgataacct atgaaataaa tgtatcaaaa gatactttta
    12181 tcaaaggaaa tgaaagttgg caaaaaccat ttggaattaa gcctgacaat aattattctg
    12241 aaatggtaaa aactatatca aacagtatta ttcacaaaga caataaagag gtgtttttaa
    12301 atacattttt gtgtaaaaat ttattatctg catataacaa aaatataact gaaataaaat
    12361 tagaatatcg aagaatgaat caagaaaata aaatgatttg ggtcatttgc acaatgcact
    12421 taataaaaga ttctattacg aatgatataa aagcacttgc ttatgttaaa gatattgaca
    12481 agcaaaaaaa tgaagaaatt aaattaaaat taaaagctga aagagatact cttacaggat
    12541 tatataataa aggtactaca gaaaatttga ttaatgattt cttgaactct gacgaagcaa
    12601 gcttaggaat gcatgcattt ttaattgttg atatagataa ttttaaatct gtaaatgata
    12661 atttaggaca tgtatttgga gataaagtat tatttgaagt ttctaaacac ctaaaaccta
    12721 tatttagaaa agatgatata gtaggtcgaa ttggtggaga tgaatttata gtattcttaa
    12781 aaaatattca gtcttttgag aataccaaaa agaaagcaga aaaaatatgc gatatattta
    12841 gaaattctta tacggaaaat aaaaagacat ataaaatatc tggaacagtt ggaatttcat
    12901 ttgtaccaga acatggaact gtatttgaag aactttataa aaaagcagac atagcacttt
    12961 attatgcaaa gagcaaagga aaagataagt atgcacttta taataattgt atctaccaaa
    13021 atgaacttga aaataatata agtgataata atataaaatt agcaaataaa tgtgagaaat
    13081 ccaaaccagt acttaaagaa attcttgatt ctattgataa ttatatttat atagtgaatc
    13141 caaatactta tgaacttctt tttgtcaata ataaggtgtc tcgtttggat ttagagataa
    13201 aattaggaga aacctgttat aaatcactat taaaatctga gagaccatgt aataattgcc
    13261 ctcttaacgg attagaagaa aataagcatt caaaattcaa aatagagaca aatacatttg
    13321 gtaaaaatat cagtatagca agttggatta attgcataga tggagaatac aactgtctta
    13381 tagagtgtat agattcttct ttatacaaaa ataaataaaa aaggactatg aagatatgtt
    13441 aaatagtata actttgatac ttggtataat atttacaatt tactattttt atctcaaaat
    13501 agtttttgga ggaatttcat ttagtgagat tttcttagtt gtaggaattg tactaattat
    13561 ctatcaaata tttaagaaaa aaattaaaga aaagaaggtt ttttataggg tgttaaaaat
    13621 aataatcagc atttttttaa tagtatttgt agttactgag agtttaatta ttttttatcc
    13681 taaaaatagt ttagaatcaa aaagtgatta tctcttaatt ttaggagctt ctgtaaaaaa
    13741 gactacacca agtacaaccc tgaagggtag gttagacact gcattgaaat acctaaaaat
    13801 taatgataat tgttatgtgg tagtatctgg tggtaaagga agtggagaaa aaataactga
    13861 ggcaaaagct atgaaagatt atcttataaa aaatagtata gataaaaata ggataataga
    13921 agaggataaa tccacgaata cttatgagaa ttttaaatat tcaaaattga agatagaaga
    13981 acatagtcaa agaaaactaa gtgatttaaa agtaaaaata gtaacaacag attttcatgt
    14041 tctcagaagt aaaatattag catatagaaa tggatataaa aatacgtctt tttatgctag
    14101 taaatcaaaa ctttcatttg taccaactta ctatacaaga gaattttttg cattatggaa
    14161 aacaatcatt tttgatagat aataaataaa aagactagat ataaactcat atctagtctt
    14221 ttctattccc ttaattctaa tccattttcc aactttactt gttgtagaaa tttcaacatt
    14281 gggatttaca agtaattgct tataagaagt tttatatttt acgattccaa aatataattt
    14341 tccttcataa tccattataa atccgaatga tcttactctt ggtttattat cttcaactgt
    14401 agcaaaatag aatgttgagt tttcttttaa aaaattcatt attttttcca tgattaaatc
    14461 ctct
//
LOCUS       DAELXX010000101        14200 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.101, whole genome shotgun sequence.
ACCESSION   DAELXX010000101 DAELXX010000000
VERSION     DAELXX010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14200
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.101"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(30..986)
                     /locus_tag="KPH29_002886"
     CDS             complement(30..986)
                     /locus_tag="KPH29_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HBG1468468.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYNFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYGYPATLFFMLCIIFIGIIIFKIKKLL"
     gene            complement(1325..2023)
                     /locus_tag="KPH29_002887"
     CDS             complement(1325..2023)
                     /locus_tag="KPH29_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1468469.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKHHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
     gene            complement(2057..4759)
                     /locus_tag="KPH29_002888"
     CDS             complement(2057..4759)
                     /locus_tag="KPH29_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HBG1468470.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKEIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEVITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSSKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            complement(4937..6754)
                     /gene="ftsH"
                     /locus_tag="KPH29_002889"
     CDS             complement(4937..6754)
                     /gene="ftsH"
                     /locus_tag="KPH29_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBG1468471.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            complement(6785..7228)
                     /locus_tag="KPH29_002890"
     CDS             complement(6785..7228)
                     /locus_tag="KPH29_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG1468472.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            complement(7719..8609)
                     /gene="metA"
                     /locus_tag="KPH29_002891"
     CDS             complement(7719..8609)
                     /gene="metA"
                     /locus_tag="KPH29_002891"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBG1468473.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            complement(8639..9904)
                     /locus_tag="KPH29_002892"
     CDS             complement(8639..9904)
                     /locus_tag="KPH29_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HBG1468474.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     regulatory      complement(10014..10116)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(10170..12827)
                     /locus_tag="KPH29_002893"
     CDS             complement(10170..12827)
                     /locus_tag="KPH29_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBG1468475.1"
                     /translation="MENHKQMPWHSRATHEILESLDSSEEGLTDTEAEKRLKENGLNE
                     LRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIVTVNAVIGIVQ
                     EKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAMVPADLRLIET
                     SSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGRGVGVVVATGM
                     DTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGAFYQRPLLPMF
                     MIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGGATVICCDKTG
                     TLTLNKMTVTHIAVNGDFESGKLTSIDTASEKHPLVYKEIVYAGALCNDACINPDKTD
                     EILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVHNIDEKIIAYT
                     KGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKDALRVLGFAKREISSIPK
                     EDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHKVTATTIAHEL
                     GIWSEENTVISGDELDNFSDDELDEAVKNTTVFARVSPFDKLRIIQSLKRIGEVPAMT
                     GDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIKEGRRVYRNIQ
                     KVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALALGVDPPSRNI
                     MKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQTMAFCVLAFS
                     QMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFVPVFRNAFNLTVLNNL
                     EWLITIVLAIMSVIQVEVGKIIKRKKRKK"
     gene            complement(12955..13698)
                     /gene="yaaA"
                     /locus_tag="KPH29_002894"
     CDS             complement(12955..13698)
                     /gene="yaaA"
                     /locus_tag="KPH29_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBG1468476.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFGIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLRVGEFKNLYEYWGNRIYKELIKEKTAIIN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMTKYIVKNRIRD
                     IEELKKFNLEGYKYKENLSNNSEYIFVKE"
     gene            complement(13708..14172)
                     /gene="bcp"
                     /locus_tag="KPH29_002895"
     CDS             complement(13708..14172)
                     /gene="bcp"
                     /locus_tag="KPH29_002895"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBG1468477.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
BASE COUNT         4448 a         2554 c         1601 g         5597 t
ORIGIN      
        1 tataaaatca attaaacatc atctgtaatt tacaataatt tttttatttt aaatattata
       61 attccaataa aaattataca tagcataaaa aataaagttg ctggatatcc ataaatccag
      121 tttaattctg gcatatatct aaagttcatt ccatatatac cagttataat tgtaagtggt
      181 gctgatatgg ctgttaacaa ggttaatttt gtaattaagt tatttgtttt ctcacctact
      241 ctggaagaat aaatatctaa taatttatct gctaactctc gtgttgacaa tgcgaaattg
      301 tataatttgt caattccaaa atctatacct tgaaaatttt ctaaattata tttttttaca
      361 tttgaatatt ttaaatatct tatattttcc ttaactattc tatccccaat atacaaaaga
      421 ggtctagtat tcttaactat aattcttgtt aatcctctta catcacttat tctagataca
      481 tgattcttat taatgttgtc catcatttca tcttctattt gaagaattaa ctcttcaact
      541 ttttccaaag actcaaaacc attttttata acttctctaa gaatcaagta gtttatctta
      601 aatagattaa taactggatt tttttcaagt tgagagttct tcaaaataat attttttaca
      661 aattcaaata aaaagtgttc ttcattcact acaaccaata taaaattatc tgataaatac
      721 atatttactt cttctataac agcttctcca tctcttagtt caaatgtatt taggcttaaa
      781 aaatcgtaat tatcaaataa atcaagtttg gttatttcat caaatttaag acaatcatta
      841 aaagttattt catctatatc caaagtttcc ttaagcaatt ttaaattttt tggagcactt
      901 aatataagat aagaattttc tgctacataa aatttatctc taaaatcttc aatagtctct
      961 cttgtattta aattcagtat atacatacat ctactcctaa atttcaattt acattctaaa
     1021 aattttttat aatccttact gtaattattt tacactattt taacaattat tatttaatat
     1081 ttacattaaa tttattataa ggtaagtgtt aaatacattt attatttaac caaagttttt
     1141 attacagatt acactatata cttattttaa aacatatttc atattattga tactaaaaag
     1201 ctaaaaaata aaatgcatgt gctaaactga caacaagcac atacacttca ttcttatttt
     1261 aactaaacta tataatttta atatatatta ataagcacat aatctaataa ttaatatatt
     1321 acaactattc atcatttaat ctgtatccta caccaacttc tgtaattata tatcttgggt
     1381 ttgcaggttg tttttctatt tttcgtctta atgttgccat aaagaccctt aaagacttag
     1441 tctcattccc tataacacta ccccatattt cttttattat aaaattatga gtaagcactt
     1501 ttccatgatg ctttgcaagt aatgaaagaa tattatattc aataggtgtc aaatggactt
     1561 caacatcatc aacaataact tttcttttat caaaatcaat caataattta tctacttcaa
     1621 acgtagattt aacatcatct ttttgttgag cttctacttg tgaatgtcta aatgcgactc
     1681 ttactcttgc taataactct actatggaga atggttttgt taagtaatca tctgcccctg
     1741 catcaaatac ttctacctta tctctatctt gctctctagc tgaaactaca attattggta
     1801 tatttgaaaa ttgtctgata gcttttatta cctctattcc atccatatct tctaagccta
     1861 aatctaaaag aacaacatct ggagaatatg agacagcaat agatatacct tcttttccag
     1921 ttatagcctc cttgacatca taatcctgtg ttaaaagtgc agtagatata aattttctta
     1981 ttgtcttatc atcctctact aacaatataa gttttttact catttcttcc tccaaaacta
     2041 attttaaaat ttaaaactat tcattttcct taggcaaact aaacttaaaa gttgaaccct
     2101 tatctttatt gttttctacc ataatttcac ctttatgagc ttgaattata gatttacaaa
     2161 ttgataatcc aagtccaaca ccctttctgg aatctttagc tccttcttct ccagtaaaaa
     2221 atctgtcaaa gatatgcttt ttaagttcct ttgatatacc aggaccatta tcagatactt
     2281 caaaccaaac ataatcatct ttctcgtata catttatctc tatcttacaa tcttcctttg
     2341 aatatttcaa agaattatct attaaattaa ttaaaacttg ttctattaat tttgcatcca
     2401 taggaacaaa taatatctgt tctggtatat taatatctat aagtgcattt tcaattcttt
     2461 tttttatttt ttgtaacgct tctgatataa tttcctcaac cagttcagaa tgttttttaa
     2521 cttccaattt cccttcatct atcttggtca tactaagtaa attttcaaca agtcttatta
     2581 accactgagt atcttcatat acaccactaa gtaattcatc tattatatct tgacctatag
     2641 tacccttgtt ttttataatt gtacttactg caccagatat tcctgctagt ggagttctaa
     2701 ggtcatgcga aactgctctt aaaagattac ttcttaatct ttccctttca atctcaagat
     2761 tggtattttc tttagcttct gacaagattt ctctatcaag cgctattgcc atttgagcaa
     2821 ttattgtttc aattaagctt atatcttctg tatccaattt tgaatcaata catgatattc
     2881 ctactactcc taaagttaaa ttcattccta caattggtat atagtatccc tttgaaccag
     2941 gaagtgtatc cgtgtcattt cctgccactg ttgaattgga taaaacccaa cttgcaacag
     3001 ctttttcatc ttcactatcc aaatcatttt tatccttatt attagtattt tttttgtaaa
     3061 gttttgattt attatgctta tctacattta aataacaaat tacatctcta cttaagctaa
     3121 gagacaaaag ttctatacct ttgtttacta tatcatcttt acttgataat cttaaaaatc
     3181 ccttaattat ttgatatatc atttttgtat tttcttctct tttagcagca gtttctgctt
     3241 catgctgtat cttagacgta agagtgctag ttattatacc tactattgag aatacaacca
     3301 gtgttactat atagtttgaa tctgctatag ataatgtata taatggtgct gtaaaaaaat
     3361 aatttaaaag aactatatta aatacagaac ttattatacc tgtactatac cctttagtcc
     3421 acatagaaac taaaactaca cccaacatat atattaacaa tatattttct cttataaatc
     3481 ccatgctttg tactgcatat gctaaaatac ttatcatcaa agtaataaat ataagtttta
     3541 taaaatcaac tttgctaatt ttaaatttag attttattga tgatttatcc tttttaggtt
     3601 tgtatttaac atctgaggca aatttatatg gaattatatg tatatctata taatcaactt
     3661 catcaattaa tttgtcaact atatcttttt taaatctttt actaaatctt ttattacttg
     3721 aatgatttct acctattaca atttttgtta catttctcag ttttgcaatt ctaagtattt
     3781 gctctattat attttcacca tgtaaaacaa ttatttctcc acctaatttc ttagctaatt
     3841 ttaggttatt ttgtagttgt ttttgttcat caaatggaag tcttgctgtg tttggagttt
     3901 caacatataa agctatccat tttgcaaatg tactgtctga tattctggat gcagttctaa
     3961 taacttttgc tgaagatgga gatggactta tacatgcaag aagtgtatct gatgtaggta
     4021 ttacagttac atcacccttt gatagtctgg tcatttgaac ttctttatta actctctctg
     4081 ctgtttttct aagtgcgatt tctctaagtg caaccaggtt atcttttata aaaaaattat
     4141 tttttgctct agtagcttgt tctttcttat atatcttacc ttcattgaac ctttcaagaa
     4201 gtacatcagg ttcaacatct ataagttcta actgtgtatt tgtatccaaa aacttatctg
     4261 gtatagtctc tttaactgat acgtttgtaa ttacctcaac aatgtcattt agactttcta
     4321 aatgttgtac atttacagta gtgtagacat ttatgcctgc taacagcaat tcttctatat
     4381 cttgccatct ttttgtatgc cttaacccag caacattaga atgggcaaat tcatctacta
     4441 atataacttc tggttttctt gctaaagcag catctaaatc aaattctttt agttttatag
     4501 atttatattc tatctccttt acaggaagaa tttccaagcc atcaagtaga gccaatgttt
     4561 ctggtctagt gtgtggttca atatatccaa ctactacatc tacacccact ttttttaaat
     4621 tttgtgcaga ttccaacata gagtaggttt ttcctactcc agctgcataa ccaaagaata
     4681 tttttaattg acctctactt agtttttctt cttcattttt aattttttct aatagaatat
     4741 caggattagg tctatccatt aaaaaacctc cttacaccat tcattactat tataatatca
     4801 attccgatat aaaattgtag ttaagatgta tttttaatga ttaagattat gtaaagattt
     4861 aaatatgaat ttaaaaaatt tagttgtaaa ataaaaatgc ctaacaatag gcatttttat
     4921 cttgtgtata atcattttag tttaagttaa gtatttccat aaactcatca cctgtaatag
     4981 tttctttttc taataaataa ttagcaagtt catgcaattt atcaatatta ttttctaaga
     5041 tttcaattgc atttttgtga caagccttta ctattttaaa tacctcttca tctattttag
     5101 cagcagtgtc ggaagaacaa gcaagagaag tatctcctcc taaatattga ttgcttacag
     5161 tttcaagagc gaccatatca aactcttcac tcataccaag gcgagtaacc atagctcttg
     5221 caattttagt agcttgttct atatcatttg atgctcctga tgtacaagtg ttaaagataa
     5281 cctcttcagc agcacgacca cctgtaaaag tagttatttt atcaattgct tcttctttag
     5341 acataagtac actctcatct tcagcaactt gcatagtata tcccaatgct cctgaagttc
     5401 taggtattat agtaatctta tggacaggtg cagaatgttt ttgcatagca gcaacaagtg
     5461 catgacctat ttcatgatat gctataattt tcttttcctt atctgaaatt acagctcctt
     5521 tacgttgata cccagcaatt acagtttcaa cagattcatc aaaatcctgt tgttctacta
     5581 tatctctgcc agacttaact gctaaaagtg ctgcttcgtt tacaatattt gcaagttctg
     5641 cacctgatgc tcctgatgta gaccttgcaa ttgcattata atctatatct gagcttacat
     5701 ttacattttg agcatgtacc tttaatatag cttcacgtcc tccaaggtct ggaagttcaa
     5761 ctggtatacg tctatcaaat cttcctggac gaagtaatgc tttgtcaaga ctgtcaggtc
     5821 tatttgtagc agcaagtatt actactccaa ttccaccatc aaaaccatcc atctcactta
     5881 acaactgatt taaagtttgt tcacgttcat catttccacc taatccactt ccattatcac
     5941 gctttttacc aatagtatca atctcatcta taaacactat acatggagct ttttcttttg
     6001 cttgtttaaa taaatcacga atcctagaag ctcccattcc tacaaacatc tcaacaaact
     6061 cagaaccaga tattgagaaa aatggtacat ttgcttcacc tgctacagct tttgcaagga
     6121 gtgtttttcc tgtacctgga ggtcctacta aaagtgctcc tttaggcatt tgtgctccta
     6181 tctttttata cttttctgga ttgtgtaaga aatctactat ttcagttaaa gcttcttttg
     6241 cctcatcttg accagctaca tcattaaatc tttttccact ttgagcagat acatacattt
     6301 tggcgttgct ttttccaaat tgcatagcat taccacccat tcgcttttgc atagacttca
     6361 tcattaagtt accaaatagc ataagaatac ctataggtat tatccatgta agtaaaatac
     6421 ttaaaagagg tgaattttcc ttaggaataa ccttagtaaa ttctactttt gcatttttta
     6481 gtctatctac caattctggg tcatccattc taccagttat gtatatattc ttatccttgc
     6541 tactagatgg atttattgct attctattat tttctatctc aacagattta acctttcctg
     6601 tatccaacat tgtgagaaac tttccatagt caatctcaac cattttttta tccattaaac
     6661 ttggaaatat taaagcattt aaaaacatta ctactattat tataactgta taataataga
     6721 gtgctgacct tttttggggt ttatgttcat ccataatttt attctccttt aaattatttt
     6781 tatgttattt gcaatcaatg taagttaaaa gtttatttaa tttctctaaa ccttctatta
     6841 tatcttccat atcttgcaat ttttcactgg tcatattttc aaaataacta tcaaaaatac
     6901 tacttgaaat atgttcttgt atatccaatg atttatttgt aacttttact tttacaattc
     6961 gttggtcttc tatatctctt atgcgattta aaaagccatt tttttcaagc ctttgacaga
     7021 ttactgatac attactattt gtcatgttta agttcttccc taaatcgctt atttttttct
     7081 ctccatgttc acataactca gataaaatca aagcttgcac tgctgtaata ccaagtggct
     7141 gataaaaatc acttaataaa gttgtaaatt tcatatttat atctctagtc aactctagta
     7201 gctctttctt aaaactcatc tcatccatac aaacgctccc tacgaaaata ctttatttca
     7261 aatttaggct gaagtgcata ctgtaaataa atacttaata ctataatgtt taatgtatta
     7321 aatattatag tattaagtat ttattgtcaa ttacaatatt gtaacttcaa ttggaatttt
     7381 gttatcttat tgatatttaa ttttatacag gatattttta ataaaattta ataaaaaatc
     7441 gatatatata ttttacactg atttctccct ttttaatctc ttttaaaagt taaaatacaa
     7501 atttattgat actataaagg tttagtctcc cattatcgca ccatatataa aattaaaacc
     7561 acctctatct acatacacct aaagtgattt tcttaaaata taaaactact attataaggt
     7621 taaatttaaa aaaaataagg tataaagtat cattaagatt aactttatac ctataaattt
     7681 ctatattttt ttagttaaca tttatatact cttaaaattt attgtatcaa atagtttttt
     7741 atccaatttc taaacaacaa ttctgaatgt tttttccatt ttacagttgg ctcttctgaa
     7801 gggtcatcat taatatagta attttgaggt atagatattt tatctccttt atttatatcc
     7861 ctcttatatt ctttatcaag agtgtatttg tcatactcgc tatgacccat tacgaacaca
     7921 tctcttgaat ttgtaattat atagggacca gcttctttag aatgactcac aatgctcaac
     7981 tctttattct ctttcaaagc ttcaatattt acagtagtat gtcttgaatg tggagcaaaa
     8041 aattcatttt caaatccatc tactatttta ctatctttat ctaccttatg cttaaatact
     8101 ccaaaacatt tttgacttag tggaagtttt tttacattgt agtatttgta aagacttgct
     8161 tgagctgccc aacatatata taatgtagat ttagtttttc tcttagaata atccataatt
     8221 tcttctagtt cattccaata atcaacttct tcaaactcca tttgttcaac tggagctcct
     8281 gttattataa agccatcaaa atactctcct ttggcttcat caaatgtttt ataaaattct
     8341 ttaacatatt cattacaact tcttttactt tctcttgttg aaattttaat aaattcaaca
     8401 gacacgttga atcctgtttt atctatcctt cttagtaaat ctaattctgt atctatcttt
     8461 ataggcatta gatttagtat agctatttta gtgtctatgt tttcttcata ctttaattcc
     8521 tttttaaaat cttctttata aattacatct attccttcat ctaataattt attaataaca
     8581 ggtaatccct ttggtagtat taaagccata tcttttcact tctctttctc ttagaatatt
     8641 agaaatttaa agcttgattt aaatcagcta ttaaatcctc tacattttct attccaacag
     8701 ataatcttat tagtgaaggc aatactccag atgcaattaa ttgttcttct gttaattgtc
     8761 tatgagtagt tgaagctgga tgtataacac aagttcttac atctgaaaca tgtgtaacca
     8821 aagatgctat ctgtaatttt tccacaaatt ttttggcatt ttctaaccca cctcttactc
     8881 caaatgataa aacaccacta catcctcttg ataaatactt tttggcattc tcataatact
     8941 tgttatcttc aagtcctggg taattaatcc aatctacatt ttcatgtttt tctaaaaatc
     9001 tagctatttt aagtgcattt tcactatgtc tctccattct aagatgtagt gtttcaacat
     9061 ttaaattagt aagatacgca ttgaatgggc ttaaacaatt tccatagtct ctaagcaact
     9121 gaactcttgc tttagttgca tatgcggcat ttccaaattt ttgagtatag cttattccat
     9181 gatatgttgg gtctggctca actaattctg gaaattttcc attatcccag ttaaagtttc
     9241 caccatccac tataattcct ccaacacttg aagcatgccc atctaaatat tttgtggtag
     9301 aatgagtaac tatatttgct ccatacttta aagggttaca aagcactgga gatgctaatg
     9361 aattatcaac aataaatgga acattaattc tcttagctac atttgctatt ttttcaaaat
     9421 ctatgacttc aagagtcgga tttgcaagtg tttctgcaaa tacaacctta gtattttcct
     9481 ttgcaagttc tactatctca tcttcacttg aatctaagtc aaacgatatt aaatctatac
     9541 caaatttttt aagactaggt cctagtaaat tgaatgttcc tccatatact tttgaagaac
     9601 aaagtatact atctcctgat ttacatatat ttaaaactgc caacatattt gcagattgcc
     9661 ctgatgatac agctacagaa gatactccac cctctagtaa acttattttt tcttcaaaag
     9721 cttgaatagt aggattgctt attcttgaat atatatgtcc atcaactttt aaatcaaata
     9781 attcagcaag ttggtctata ctgctatatt taaaagttgt actttgatat aaaggtaata
     9841 ctcttggttc tccattacct ggtttataat ttccttgcac acatattgtt tctttattat
     9901 acattcttgt atccccctta gattttagta aaaaaaatta aactatttat cagatgagag
     9961 ttaaaataaa taaagcctga gtatataact caggctctgt ataagcttaa ttatactcat
    10021 ctctcagctt tcgccgcagg atttagcacc aatgtatata aatatactgg ttgccgggtt
    10081 tcatagggcc agtccctcca cctctctgga taagatttat ttaatttttc tatgttttat
    10141 atcttatata tttttcctaa ttttgtcaac tatttttttc ttttttttct tttaataatt
    10201 ttcccaacct ctacttgaat tacagacatt atggctaaaa caatcgttat aagccactct
    10261 aagttattta aaactgttaa gttaaaagca tttctaaaaa caggtacaaa cagtattact
    10321 gctattaaaa atgcagaagt taaaaatgag attaaaagaa atggattttt accattattt
    10381 ctattaaaca ttgaatctgt atttgaacgc tgactaaatg ctcgtaacat ctgtgaaaat
    10441 gctaatacac aaaatgccat tgtttgacct acagaatgat tttctatatt aagaccaatt
    10501 ttatatgcta aaatagtcat tattgccaca aatatacctt gggtaacaac tctttgtata
    10561 agattttttt caaacaaagt ggttgatttc actggctggt gcttcattat atttctgctt
    10621 ggtgggtcaa ctccaagagc aagggctgga agcgtagcag ttgcaaggtt tacccataga
    10681 atatgaacag caagaagcgg agtatcccaa tttaataata ttgctacaaa caacgtaagt
    10741 atctctgcta tattaccaac aagcaaaaat tgtataactt tttgtatgtt tctatataca
    10801 cgtctacctt cttttattgc ataagcaatt gttgtaaaac tatcgtccat taaaatcatg
    10861 tcagaagaat cttttgcaac atctgtacca cttataccca tggctatacc aatatcagca
    10921 gactttaagg caggagagtc atttacacca tccccagtca ttgctggtac ttctccaatt
    10981 ctttttagag attgtataat tctaagttta tcaaacgggg acacacgagc aaatactgta
    11041 gtatttttaa cagcttcatc tagttcatca tcactaaaat tatcaagttc atcaccagat
    11101 atcacagtat tttcttcact ccatattcct aactcatgag cgattgttgt ggcagtaact
    11161 ttatggtcac ctgttatcat tatggttctt atacctgctt ctttacaagt acttactgca
    11221 tcaattacct cagttcttgg tgggtcaatc ataccaacca tacctataaa tgtcaaatca
    11281 tattctatat tttcactatc ttcttttggt atagatgaaa tctcacgctt agcaaaacca
    11341 agtacacgca atgcatcttt agacatgtta agacataatt cgtgtatatt ctttttatcc
    11401 acttcggtaa tgtttctttc accttttgaa gttaatattt tagtacatag gtcgagaatt
    11461 tcgtctactg ctcctttagt ataagcaata atcttttcat ctatattatg aacagttgtc
    11521 atacattttc tatctgaatc aaaaggctgt tcaaatacac gtggatattc ttcttcaaga
    11581 gcttcttgat ctattccaaa ttttttggcc ataaaaatta atgcgccctc tgttgggtca
    11641 cctaatattt catctgtttt atctggatta atacatgcat cattacaaag tgcaccagca
    11701 taaacaatct ctttatacac taatggatgt ttttcagatg cagtatctat tgatgtcaat
    11761 tttccagatt caaaatctcc attgacagca atatgtgtaa cagtcatttt atttaaggtt
    11821 aatgtaccag ttttatcaca acatattact gtagctcctc ctaatgtttc aacagcaggt
    11881 aattttctaa ttaaagcatt tcttttagcc atacgctgta cgcctaaagc cattacaata
    11941 gtagctgttg caggtaatcc ctctggaata attgatatgg caagggaaat tgcaatcata
    12001 aacattggca agagaggtct ctgataaaat gcacctattg caaatataac aacgcataca
    12061 ataataccta tgacacttaa tgtctttcca acagtattaa gtttacgttt tataggagta
    12121 tcaaaatcat cttgtgaatc taaaagatgt gctatgttac ccacttctgt atccatacca
    12181 gttgctacta caacacctac acctctacca tatgtgacta ttgaagatgt atacgccata
    12241 ttgctacggt ctcctaatac acattcttta ggtaagattg tatctgcatc tttttctgat
    12301 ggaacagatt ctcctgttaa agaagcctct tgaattttta aacttgatgt ttctattaaa
    12361 cgaaggtctg ctggaaccat agctccatct tctaaaaaaa caatatctcc tactacaaga
    12421 ttacttgctg gaataacatt ttcttcacca tcacgcataa cacgagctgt aggtgagttc
    12481 atatttttta atgcttctaa tgatgattct gcttttttct cttgaacaat tccaataact
    12541 gcatttactg ttacaattat tagtattaca aaagcttctg taaattcgcc aaatatcaaa
    12601 gataatattg ctgctcctat tagtatcata accatagcat ctgtaatttg actccaaagc
    12661 atttgtaaag ttgttctagg tggttttttt ctaagttcat ttaagccatt ctctttaagc
    12721 cgtttttccg cctcggtatc ggtgagcccc tcctctgatg aatctagact ttctaaaatt
    12781 tcatgcgttg ctcttgaatg ccaaggcatc tgtttgtgat tttccatttt ggaagaccac
    12841 tcctttcttt ttttaattta agaataatta tattttaaaa tcaagttcaa ttcaatacac
    12901 actaagtata ttttacctaa atttaacaat gccatttaat atttaacaaa acttctactc
    12961 ttttacaaat atgtattcac tattgttaga taagttttct ttatacttat aaccttctaa
    13021 attaaacttt ttcagttctt caatatctct tatcctattt tttacaatgt attttgtcat
    13081 catacctcta gctttctttg cttgtgtaga ttccactttc aatatgccat ttttatttac
    13141 tttaaaagta catgtaatat atgtatcact atctttaata aatttttcta tggacttgct
    13201 gtattcattt gatgaaagat taatgatagc tgttttttct ttgattagtt ctttatatat
    13261 acgatttccc caatattcgt ataaattctt aaactcacct actcgtaatt ttgtctgcat
    13321 ttctaatcta tattcatata tactatcata tggttttaca acaccataca atccacttat
    13381 gattcgaata cagctatttg caaactcttc atctttcctt gtaaaatttt ctgcttctat
    13441 atttttatat tgtattccat cataagttaa gattgcaggg tttcctaatc tatcaaattt
    13501 aatgctttca aatctatttt tatttaatac agctaatttt tcattaattt tcattttatt
    13561 tttaaaatct tctataccaa atgtctttat attttccact atttcttttg tgttatctat
    13621 aaaacaaggc aaactcaaat ctctatcaaa tacttctata ttcttcatat ttttagctgg
    13681 tgataacatt ataatcataa attcttatta ctcctgtttt tccaaatatt caagaatctc
    13741 ttgtgcattt gtgtctggtt ttgctttttc aaagactttt tcaataattc cattttcatc
    13801 tataatatat gtcgcacgta caactcccaa tgcagttttt ccatacattt ttttctcttt
    13861 ccatacatca aaagctttta tagcaactaa gtcagggtca gaaagtaaaa taaaaggaag
    13921 ttcatgcttt tctgcaaatt tttgatgtga ttttatacta tctttactta tcccaataac
    13981 ttgtatatct tcctttttaa atccatcata tgcatttcta aaagcacaag cttgacgtgt
    14041 acatcctggt gtattatctt taggataaaa atatactact acttttttgc ctttaaaatc
    14101 tgacatactt accttatttc catctttatc ttctagtgta aattctggtg cttttgttcc
    14161 tatactcaac ataaatatct ctccttttgt ttttattaat
//
LOCUS       DAELXX010000102        14047 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.102, whole genome shotgun sequence.
ACCESSION   DAELXX010000102 DAELXX010000000
VERSION     DAELXX010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14047
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.102"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..351)
                     /locus_tag="KPH29_002896"
     CDS             complement(<1..351)
                     /locus_tag="KPH29_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HBG1468478.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDRKFN
                     IKGRVCLKNAIYFGV"
     gene            complement(466..1425)
                     /locus_tag="KPH29_002897"
     CDS             complement(466..1425)
                     /locus_tag="KPH29_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG1468479.1"
                     /translation="MIESKKSEKLLLSIIYIIFLVYILMIIRIILFRDVPIYAIFKGT
                     FRNVNLIPFYTIYQFIVDSNLDFMKATVNIIENIGIFIPMGIFLPIVCKNLNKKTIII
                     VIILVSLAFELTQYIFALGSSDIDDVILNSLGGIIGINIYINMNKLFPNDIKRFKAII
                     ATSLVLGVLGLGVISKNYHNLLTFKFRPDRKISKILIEENREIIKDINKDTVDIVGTF
                     ESFKKGIITIKAGSNNKVKRPENLIDSDGNIRIYLNENTKLVSYIITKESKMDIVKYE
                     EFDVKNLDLLRRYDTINVWIDKENQPKDKHGIMASKLLIGLYE"
     gene            complement(1583..1900)
                     /locus_tag="KPH29_002898"
     CDS             complement(1583..1900)
                     /locus_tag="KPH29_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468480.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTLRLF
                     VIQ"
     gene            complement(2007..2510)
                     /locus_tag="KPH29_002899"
     CDS             complement(2007..2510)
                     /locus_tag="KPH29_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG1468481.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVIHS"
     gene            complement(2562..3761)
                     /locus_tag="KPH29_002900"
     CDS             complement(2562..3761)
                     /locus_tag="KPH29_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaX-L"
                     /protein_id="HBG1468482.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIENDDVINVYEDGKNIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEENKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAISLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            complement(4021..4209)
                     /locus_tag="KPH29_002901"
     CDS             complement(4021..4209)
                     /locus_tag="KPH29_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468483.1"
                     /translation="MNYHTKEELMEVLRVASSRIINCEKVQKKFSEETSHHTRFKNII
                     EAMYISKSLIMDEISKRD"
     gene            4544..5512
                     /locus_tag="KPH29_002902"
     CDS             4544..5512
                     /locus_tag="KPH29_002902"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBG1468484.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTKL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPNTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLRECIALSKTL"
     gene            complement(5629..6759)
                     /locus_tag="KPH29_002903"
     CDS             complement(5629..6759)
                     /locus_tag="KPH29_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1468485.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPNRKVMSDINISDIESLAYKTREFWDLNSTPIVNMVSLLES
                     KGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLKDLTHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     SLEEFGINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDGKK"
     gene            complement(6912..7865)
                     /gene="msrA"
                     /locus_tag="KPH29_002904"
     CDS             complement(6912..7865)
                     /gene="msrA"
                     /locus_tag="KPH29_002904"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBG1468486.1"
                     /translation="MIKLATFAGGCFWCMVKPFDKYDGVIKVVSGYTGGHVENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTPIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRIEVRSAKGNSHLGHV
                     FDDGPKELGGLRYCINSASLKFIPRENMEVLGYGEYLKLFE"
     gene            complement(7989..8894)
                     /locus_tag="KPH29_002905"
     CDS             complement(7989..8894)
                     /locus_tag="KPH29_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBG1468487.1"
                     /translation="MKSSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFKLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
     gene            complement(9258..10850)
                     /gene="hcp"
                     /locus_tag="KPH29_002906"
                     /gene_synonym="priS"
     CDS             complement(9258..10850)
                     /gene="hcp"
                     /locus_tag="KPH29_002906"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBG1468488.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKLG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDIGGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            complement(10910..11635)
                     /locus_tag="KPH29_002907"
     CDS             complement(10910..11635)
                     /locus_tag="KPH29_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG1468489.1"
                     /translation="MMRKIIKIDEGKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSTSISNDIVEIKGSQLNQWPVQIKLVPTNASYLKNASLLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            11776..12504
                     /locus_tag="KPH29_002908"
     CDS             11776..12504
                     /locus_tag="KPH29_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG1468490.1"
                     /translation="MKTNYRLHMQQGSDYLKKFLEKHIDLLKKIPLFEGIKSDELEEM
                     LECLGVVDKTYPKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLF
                     GEVFACTRLHKSNVTVTTTSSCEVLFIKFKSVTGICSSACVFHNRLVENMLQLIAEKN
                     ILLHNKIELLSRKTTREKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRE
                     LGKMRDEGLLSFNKNKFEIFYFQK"
     gene            complement(12668..13939)
                     /locus_tag="KPH29_002909"
                     /pseudo
     CDS             complement(12668..13939)
                     /locus_tag="KPH29_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416360.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
BASE COUNT         4709 a         2410 c         1443 g         5485 t
ORIGIN      
        1 tacaccaaag tatattgcat tttttaaaca tactctaccc tttatattga attttctgtc
       61 agaataatcc caccatttag catgaaataa ggtttctaaa ataaatcctg taaaatattc
      121 tagtatcgta gttaaaatca ttcctaatag aaatactatt actacattat taaatcttcc
      181 taatagtgat atcacaatta ctgctccaaa accataaaca gggcatattg gaccatttaa
      241 aaaacctctg ttgattattc ttttgcttgc tatagaacat cctatacttt ctactgtcca
      301 tcctaaaaat gaatatataa aaaagtacaa gatatatttt attatctcca taatttatct
      361 ctactccttc actaatgttc ttattttatg cacaacgtaa aaataattat agaaatagtt
      421 ttttcgtttt ttatatttgt tattctttta ttcttgttta atattttatt catacaatcc
      481 aattaataat ttactagcca taataccatg tttatccttt ggctgatttt ctttatcaat
      541 ccaaacatta atagtatcat atctccttaa taaatctaaa ttcttcacat caaattcctc
      601 atatttaact atatccattt ttgattcttt ggtaattata taagatacta attttgtatt
      661 ttcatttaaa taaattctta tatttccatc agaatcaatt aaattctctg gtctctttac
      721 tttgttatta gaacctgctt taatagttat tattcctttt ttaaagctct caaatgtccc
      781 aactatatct actgtatctt tatttatatc tttaattatt tccctatttt cttcaattaa
      841 tatttttgat atttttctat caggtctaaa tttaaaagtt agtaagttat gataattttt
      901 agaaataact cctaatccta aaactcctaa aactaaacta gtagctatta tagccttaaa
      961 tcttttaata tcattaggaa ataatttatt catgtttatg taaatattaa ttccaattat
     1021 tccacctaat gaatttaaaa taacatcatc tatatcacta cttccaagag caaaaatata
     1081 ttgtgttaat tcaaatgcta ggctaacaag tataattact ataattatag tttttttatt
     1141 tagattttta catactatag gtaaaaaaat tcccatagga ataaatatac ctatattttc
     1201 tataatattt acagtagctt tcataaagtc aagattacta tctactataa attgatatat
     1261 tgtataaaat ggaattaaat ttacattcct aaacgttccc ttgaaaatag cgtaaattgg
     1321 tacatctcta aataaaatta ttcttataat cattaaaata tataccaaaa atattatgta
     1381 tataattgac agtaacaatt tttctgactt tttactttct atcactgata agtctccttt
     1441 cgctattaat attttaagta tatcaataaa tatttaagac gattttaaaa tttccttaaa
     1501 atttccttaa ttttcaaaca aaaaatcaaa aaagcctaca cttaaatgtg tagactttaa
     1561 taaattttca tacaaataca ttttattgaa taacgaacaa tcttaatgta gaaaaaacta
     1621 taactacagt taaaagaagt ctagcatata aaaaattttt ttctttcaac ataactcctg
     1681 caactccaaa tataaaaaat agagaagcaa taaattttcc aataagatta ttattaggat
     1741 aataactaaa aagcataaaa caaagtacaa atgtaagtac tcctattgca ttaaacacaa
     1801 ttttattaga tgctattttt cctttattga aaaagaaaca gaatattgac actaaatcta
     1861 aaaataaaaa tacataaaac attcctttta aaacgcccat ttttataccc cctaaaaatt
     1921 ctaattataa tatccagttt atttcatata atcgtcaata taatcaatac tttataacat
     1981 cagtataaca gtattacaaa tttatattac gaatgtatta cttcgtattc atacatatta
     2041 taatcacact catacatatt taaagactca tatgttgctt ttgcatttat gttttctttt
     2101 tctacatata atctcattcc aactatattt tcatctttat cacatatatt ttttatataa
     2161 ttaaataaat aattaaagat accttttctt ctatattcct tatctacata tacactctga
     2221 atccaaagga aatttccatt tctccaatca ctccactcat atgtatacat aatctgtgca
     2281 acaactttat caaatactgt atatacatga tattttcctt ttctctcatc taataacaat
     2341 gcttttacac ctttagttaa aacatccata tcaagttctt ttccttcagt ttctttagcc
     2401 aaattataat taaattttgc tattgtatcc aaatcatcca tacgtgcttc tcttatctct
     2461 attagttctt cattttcata taactctatg tagtcctttt gttgctccat acttattcct
     2521 ccatatctat tttatctact aaggtttttt atataatata tttatagagt tttttctatc
     2581 ttcataaatg cataatcaag tgtagcctct aaatttccat tattcttacc tcctccttga
     2641 gctaatactt tacttccacc acccttgcca tctataagac ttattgcatc ctttaataaa
     2701 actcccatat taatatcttt taaattttta gaacatgcaa aaactaaatt tattttatca
     2761 ttaaatttaa cagctaataa tgcaatacta ttttcatatt ctgcaatttt atttgctatc
     2821 ttatttacat atttcaaatc ttcatcatca taaatttttt taatgaccga aatattgcca
     2881 accttatttg ctttttcaat catttctttg acctgatatg tagatactac ttcttcaagc
     2941 cttctttttt taattaaagc ctcttccact ttactattta gatttacaat accatttata
     3001 gcatcttcct cactacagct taaatatcta catattttag tcaaatatat atccctcttc
     3061 aatacttcat ccactgccct tttacctgct agaaactcta ttcttgtact tgacttatgt
     3121 ttttcccatt tctttatttt tatcattctc aattcaccag ttgattttgg atgcactcca
     3181 caacatgcat tcgtatctaa atcacctatt tttattactc ttatctcttc atcagtattt
     3241 ggcaaatcac gtcttaacca aatctttttt agttcttttt tcgttggaag taatgtctct
     3301 acttgtatat tttggcgaat aatttcattg gcaaacaact ctatctctct aattttgttc
     3361 tcttctaaat taccttctat atctactgta ctatactcat ttcctaaatg aaaacctgtc
     3421 gtatttgcat taaaagtttt aaaaaaacat ccagataata catgttgtcc aaaatgttga
     3481 tgcattccat cttctcttct atcccaatca atactacact ttaccttatg tatctttata
     3541 ggttttttat ctaatacatg gtatatattt tttccatctt catacacatt tattacatca
     3601 tcattctcta tttttcccag gtctccaaac tgacctcctc caccaggaaa aaaagctgtc
     3661 ttatctaata aaacatgata cttattttct acttctttta tttcaattat ttctgctgta
     3721 aaatctctta tatactgatt tgtataatat aacttttcca ttataattct atctccattt
     3781 tcttaattat agactacttt actttttaaa tacaaatttc aagctaagct taatttaatt
     3841 gtattaaaat agaactcatc ctattattta ctaacatctt ctactatatt ttaacatata
     3901 gatattagtg ttaacaaatt tataatttta tattatttca atattttata acaagtatgc
     3961 ttacttattt tcttatataa taaaatgtat attaaaacat attaaattgc aaaataagct
     4021 tcaatctctt ttactaattt catccattat taatgatttt gaaatataca ttgcttctat
     4081 aatattctta aaacgtgtat gatgagatgt ctcctcagaa aatttcttct gtactttttc
     4141 acaattaata atccttgaag atgctactct cagtacttcc atcaactctt cttttgtatg
     4201 ataattcata acacatttac tcctttgttt tttaatttag agaaacagta tacctcaatt
     4261 taatatcatt atcaactgat atcattatta tttatatata tcttttaaca cttaaatatt
     4321 ctaactatca gtgacattac atattttaat aattattaat atacatataa tactttaaca
     4381 ttcattaata gagagtatat taacattgct tttttaaaaa ttttagtata aaatcatatt
     4441 aaataaattt tctgaaataa taaaatatta aaatatccaa aaacagcttt tgataacatc
     4501 tataattttc aatgtatcgt tattttaggg ggagtgattt aaaatggcaa taaagccaag
     4561 aaaaatatct attgtaggtt cagggcatgt tggttctcat tgtggattta gtttaataac
     4621 tcaaggagtt tgtgatgaac tctttatgat tgatatagat gaatctaaat caaaagctca
     4681 agctttagat ttagctgatg cagtttcata tctaccacat aaagtacata tagaaaaagg
     4741 aactttttct gactgtaaag attctgacat agtagtgata agtgttgcag actcttcaga
     4801 agggccttta cgtagacaaa acacaactag attagattta ctaagaccaa ctattggaat
     4861 gataaaatct atagtaaaac ccatagttga ttctggcttt gatggtatat ttgtagtcat
     4921 atctaatcct gttgatgtag ttacaaatta tatttgggaa aaatctggct ttcctaaaaa
     4981 taaagtcatt ggaactggaa cagctttaga ttcaacaaaa cttagaagga ttttatctga
     5041 agaaactgga atcgctcaac aatcaattca agcttattct atgggtgaac atggagattc
     5101 tcaaatggtt ccttggtctc atgtttcaat tggtggtaaa ccaatcttgg acatgataaa
     5161 agacaaccca aatacttata gcaatttaga cttaccatca atagttgaaa aaaataaaaa
     5221 aacagggata agtataataa atggtaaaga ttgtacagaa tttggtatag gtactgcttt
     5281 agtagaaatt gtaaaagcta tactacacaa tgagaaaaaa gttcttccag tttctacatt
     5341 actagaaggt caatataacg aaagaaatgt atttgcaggt gttccatgtg ttataggaaa
     5401 agatggtatt gaagaaatta ttgaaatcaa tatgactgaa tatgagcaaa acgaatttaa
     5461 taaatcttgc tctgttttaa gagaatgtat tgcactttca aaaacacttt agattcaata
     5521 ttctttctta cactaaaagc aggtaaatga cttattgaaa agacttttaa ctaagttaat
     5581 gtttaaatca tttatacctg cttttaattt taatactaaa atataaacct attttttacc
     5641 atcaaatttt ataactttac tttttttatt tacctcttga gataacaatc cttttttcaa
     5701 tcccatcaat aattctaagt cttctggata aagatttatt ccaaattcct ctaaacttga
     5761 tactatacta tttacactca ttattttatt ttctatcaat aattcaactg ctctttttaa
     5821 atacgttggg cttgaacctt tcattttgtc tagtggttct tctatcaacc agcctttttt
     5881 atccatttca ttcataagat aattgtattt tttgtaattt atcttttcta gattatatgc
     5941 tctaaaaatc atagcagata taggtacaat ccattttttc tttagttcta cataataatc
     6001 taaatcttct ggatgagtca aatcttttaa aaaactctct tttggcaata aaaatgcaca
     6061 ggcatactca tcttctgaaa attgcttact tggtattctt aattcacttg atattatata
     6121 tccaagctca catgccaaag tataatttct aattgatgct gactttttgt cattacccag
     6181 agatattaga tatttagatt ctttactaat tctctgtttt tgagtaaaga tagttgcccc
     6241 ttttctatct acattcatac ctgatattat aatcccttta gattctaaca aacttaccat
     6301 atttactatt ggagtactat ttaaatccca aaactctcta gtcttatatg caagagattc
     6361 tatatcactt atattaatat ctgacattac ttttctattt ggcaagtttt cttttggaaa
     6421 cttaatataa ttctccataa atgaatatat tttatgtatt ataattattt tttctctata
     6481 agaaatctct tctactctag gtagtttaga ttgagggtta aaatggctat cctctacaac
     6541 gatatttata ttttcatttt tataaaaata ctcttttgga aaatttaata tattagataa
     6601 ttttaatgta ttttctatat ttggtacata cttattttcc tcaaaagctt ttaaatcttt
     6661 tttatttatt ttagtttctt tagacaactg ttctatcgtc tttgctctaa aaattctagc
     6721 tgattttaat ctctttccat taaagtcttt taaattcatt tgttttctct ctttcaaaat
     6781 taaattctta atctattggt ataaataaga ttacaattaa taatttatca tattattgac
     6841 aaaaaaacaa aaggtgaaaa tattttttta tattttcacc ttttagataa gtttataata
     6901 aatattccta attactcaaa aagttttaaa tattctccat atcctaaaac ttccatattt
     6961 tctcttggta taaatttaag agaagcagaa tttatacaat atcttaaacc tccaagttct
     7021 tttggaccat catcaaatac atgacctaaa tgagaatttc ctttagcact tcttacttca
     7081 attctgtaca taccttgtga gaaatcactt ctctcaatga tagatttatc tataactggc
     7141 tttgaaaaac ttggccaccc acatccagaa tcaaatttat cttttgaaga aaataacact
     7201 tctccactta caatatcaac ataaattccc tctttgttat tatcataata ttcattttca
     7261 aaaggtactt ctgtcatatc attttgagtg acttcaaatt gtataggagt taaagtcttt
     7321 tttaactctt ccctatttat attgtttcca tcccaatgac tttttacaaa attatatctt
     7381 ccagaccctt tataatacat attgtagttt aatggtcttt ttttataata atcctgatga
     7441 ttttcttctg cttcgtagaa tttagttgct ggaattatat cagtcactat tttactatca
     7501 aatattttac tattttccag atgttctctt gactctaaag ctatattttt ttgttcttca
     7561 tcatgataaa atatagcagt cttatattga gttcccctat caccaaattg tccaccatca
     7621 tctgtaggat taatctgttt ccagaagata tttagaagtt catcataact tattatatca
     7681 tcatcatatg ttatttgaat tgcttctaaa tgaccagtag ttcctgaaca tacctcttga
     7741 tatgtagggt tttcaacatg accacctgta tatccagaaa ctactttaat aactccatca
     7801 tacttatcga aaggttttac catacaccaa aaacatccac ctgcaaaagt agcaagtttt
     7861 atcattttca tcactcctta aataaattat actttattta ttccctaaga agtaacttaa
     7921 taatctgcat tttattattt ttacctactc aatgaaatca tgtgcaaaaa tacaattgca
     7981 aattgaatct aatttctata aaagctcttt ttctttatcc atccaaaagc atcttttata
     8041 tcacaatttt ctcctaaaga cattagatat tctaatgcat cacaatcatc catagctaca
     8101 tctttttcca tcttaggatt taatctatag taattatctc caagaagttt ttgagaaaat
     8161 aactgactca tttgagaatt tccttctaag gtaatagata taattggtaa atcgggttct
     8221 ttattaatta tccagtcaat agctccccat tcagtagtat ctgcctttat actattatat
     8281 acatatccag tccctattga aaaaagtctt gtattcttaa gtttaaatcc actgtcaatg
     8341 gcatgaatta tacacgctaa acttgggtca gttgctatta ttcctccatc aacatgacga
     8401 ttatatgttg gaaaaaatac tggagcagca ctacttgcca ttgctacatc tacaactttg
     8461 ctagtctttg taaaagaatt tggcatattg ttataaaaca ctggtttcca tgcattttcc
     8521 tcatttccta tataaaaact tggtatcatt actaatttat ttaaatcttt taattctagt
     8581 tcctctggaa atatacttaa taaaacttct tttaagtttt tgttttcata ctttggtctg
     8641 agtatttctg cataactttt attaaaaata tacttagaat tttcaattga gtaaagttct
     8701 tttatctctt tagggcttac accatacgca agtcctagtg caattagact ccctgtagat
     8761 gtccctccaa acatatttat attgtttaat agatttggat actcttttac tatcctctca
     8821 agtatggata tactcaatgc cccttttaat ccaccaccat caaatgccaa tatattaaaa
     8881 ttagaacttt tcatatacat atacccccac ataaaacacc ttatatttta atcaaaataa
     8941 tttcactata gtatatatat tttatgcaag ttacatatta caatgttcct cttttaatat
     9001 ttaagttaca ttcaaacctt tagtacttaa aataaaatat ataagatatt atatatctaa
     9061 gtattataaa tttaatacaa aaattcttaa aagaatatac tgaaagcagg tttatttttc
     9121 aattattaca aaaaataaat aaagggtcgt ctcaaaattg attttttaat caaattttaa
     9181 gtttaacccc ttatcttaat tgtgacatac ttaaaaatat tttaactata aatatcttta
     9241 agtatagata tatttattta tcctaaaatc ttcttcatat cttcttcagg agtagatata
     9301 ggtgtcaatg caaatttatc aaccaatata tctaatacat tcttagataa gaatgctggc
     9361 aaagaaggcc ctatatagat atttttgata cctaaatgaa gtagtgtaag aagtatacaa
     9421 acagctttct gctcatacca tgacagtacc atgctaagag gcaaatcatt tactccacat
     9481 tcaaaagcat ttgccaatga aactgccact ttaattgcac tataagcatc attacattgt
     9541 cccatatcca ttaaacgagg taagccacct atatccccaa ggtctaaatc attaaatcta
     9601 tattttccac atgcaagtgt caatataaca gtatctttag gagcttgttt tacaaattca
     9661 gtgtaataat ttcttccaag ttttgcacca tcacaacctc caaccaataa aaagtgtctt
     9721 atagctccac tctttacagc atctactact ttatcttgta cagatgaaac tgtacctctt
     9781 gcaaaaccag tagttaatgt atctcctcca ttaataccag tcatatgaac atcttctcta
     9841 tatcctccaa gttcaagagc tttttctatt acactagaaa aatctttatc ttcatcaata
     9901 tgaaccatac ctggataagc aactacttct gtagtaaata ctctatctct ataactatct
     9961 ttaacaggca ttaaacagtt tgttgtaaat aatattggag caggaatatt atcaaattct
    10021 ttttgttggt tttgccatgc tgtaccaaaa ttacctttta gatgtttata ttcttttagt
    10081 tttggatatg catgtgctgg taataactca ccatgagtat atatatttat acccttatct
    10141 ttcgtttgtt ctaataattt gtgtaaatca taaaggtcat gtcctgtaac tacaataaat
    10201 ggccctttct cgattttcat agatacctga gtcggttctg gtgttccata agtttcagta
    10261 tttgctctat caagcaattc catacatttt aagtttattt ctccagtttt cataactaga
    10321 cctaaaagtt cgtcaataga caagtctcct ccaactgcta ttaatccttc atagaaaaat
    10381 tcatttacgt ctttgtctgt ataaccaagt accattgcat gatatgcata ggcagccatg
    10441 cctcttaatc caaataagat taatgatttt aatgaacgta tatcctcatt atcattccat
    10501 aaatccttca tatcatagtt atcatttcta ttacaagttg ttgaacaatc tgaacatcta
    10561 ggtacaagtt tacttttttc tacctcagtt ttttcaattt gtctttttat agcttcatca
    10621 tcaaaactta cattcgttat agtagtgaat agcccctcta tcataatttt atctgtttca
    10681 cttgttggtc tattcttatt tccaatagtt gctcttgaaa gaccaatcaa cactcctgta
    10741 agctcgtctt gcaagttagc tgtacccccc tttttaccac aaacaccatt tatagtacaa
    10801 cctttacctc ctgctgtttg ttcacattga aaacaaaaca ttttttccat tttttattcc
    10861 tcccttttta tttattaaaa tttattatat ttttctatga ttcacttaat taaatttctc
    10921 catctacaat ttttccatct acagatatag taactatttg ccatggtatc atttttccac
    10981 tattttgtag agctgtctta acagcattta ctataccacc acagcaagga acttccatac
    11041 gtgttactac tatattttta atatcatttt cttttaaaat ctctgtcagt ttttcagcat
    11101 agtcaacttc atcaagttta gggcatccga ttaatgtaac cttatttttc ataaatttat
    11161 tatgaaaatt tccatatgca tacgctgtac agtctgctgc tattaaaaga cttgcattct
    11221 ttaaatatga tgcattagtt ggtactaatt ttatttgtac tggccattga ttaagttgag
    11281 aaccttttat ctctactata tcattgctta tagatgtact atttgaaacc tctctattta
    11341 ttgacttaga ctgagaacct ggacatccac atgctaaagt tttcttttgt gctttctttg
    11401 cttccatatt tgcttttact gcagcttcat catattctgc tgcttctcta tactcaaatg
    11461 aaatcgcatt tgttggacaa gttggcagac aatctcccag cccatcacaa tagtcatctc
    11521 ttaaaagttt tgcttttcca tttaccattc ctatagcttc ttcatgacaa gcttcaacac
    11581 atagtccaca accattacac tttccctcat caattttaat tatttttctc atcatttata
    11641 tcacctcata aaattttttt aatttaaagt attgatttct tatatattta tttttaatac
    11701 ctatatttct ttatttcaaa tttcttgtag taggattttt atatcccctt gcttttataa
    11761 acttagtata gtacaatgaa aacaaattat cggttgcata tgcaacaagg aagtgattat
    11821 ttgaaaaaat ttttagaaaa gcatatagac ctacttaaaa aaattcctct atttgaagga
    11881 attaaaagtg atgagcttga agaaatgttg gaatgtttag gtgttgtaga taagacttac
    11941 ccaaaagata gtataatatt ctctgttgga agtgaagtta cttctattgg aattatgctt
    12001 aaaggaagtg ctcatataat aaaggaagat atcgaaggga ataggaatat tgtagctgaa
    12061 ctcttgcctg gagatttatt tggagaggta ttcgcatgta ctagactaca taaaagtaat
    12121 gttacagtaa ccacaacatc ttcttgcgaa gttttattta taaagtttaa aagtgtaact
    12181 ggtatttgtt catctgcatg tgtatttcat aataggcttg ttgaaaatat gctacaatta
    12241 atagctgaaa aaaacatttt acttcacaat aaaatagaat tactttctcg taaaacaact
    12301 cgcgagaaat tgcttatgta tttttcaaaa cagatagaac aaacaggtag ccaccagttt
    12361 acaattccct tttcaagaaa tgaaatggct gattttttat gtgttgacag aagttctatg
    12421 tcaagagagc ttggcaagat gcgagatgaa ggcttgttat cttttaataa aaataaattt
    12481 gaaatctttt actttcaaaa ataaactaga tatttaaaca tatttatttc aatatatact
    12541 atattagtta attcaaatta taaatatcat atttgaatct taaatttata tttttactta
    12601 aaaagttaat agatatctaa tttattaaaa ttaagatacc tattaacttt attaattaag
    12661 tgtacaatta gcttattttc tttgtagtaa ttgctgtttt atctataagc caatcttttc
    12721 cttctactaa tcttgaaact aacattgatg atactgtatt tccagttgta tttaatatag
    12781 tattaggaat atcagttata gtagttataa ctgttaacaa tggaattgcc tccactggaa
    12841 aaccgaagat gctaaccacg agcatctcac caataagtca gccacctgct acaggagcca
    12901 taaatactcc aacaacaaat acagttataa ataattgacc tatcgttgct ggatttgcca
    12961 ttccactgcc taaaatacct gataaaaatg ccacttttat tattactgct aaaactgacc
    13021 catctttatg tatatttatt ccaagaggta taactgtttc tgatatatca tcaggaactc
    13081 ctatattttt actagcttct aaatttgatg gtatacatgc tgaacttgaa gaagtagcta
    13141 gtgccgttgc tattggtttt atagcatttc tccagaattg tctaaaccct tgttttcctg
    13201 cagataaaaa agcatacaga ctgtaaaata ataagaaata catggctgct gttagtgaat
    13261 aaattacaaa cacccttaca tatccttgaa ttattttgct tccaagttcc ccaattattg
    13321 tagctgtata acagcctagc cctataggag cataaagcat gactatactt actactttta
    13381 atattgcttc cgttccttct gataaaaact ttcttattgt atttccccta tctcccatca
    13441 tagctattgc tgttccaact aaaactgcaa atataactaa ttgtagtaaa ttgctactgg
    13501 aaaataaatc tccaaaattt ttaactgtaa tacttgatac tacttggtct aataacgttc
    13561 cttttgattc taaagtttct gcatcttgag ccaccattaa actttttaca cttgatacat
    13621 ctaatccttt agacggatta aataataatg cagatgtaat acctaaaaat gcaattacaa
    13681 gtgttgttcc cataaatact atagctgtac tcttcattat ttttccaaaa cgttccatat
    13741 tttttatact agcaacacct gaagaaacac taagaaaaac taatggtact actatcatta
    13801 atagagcatt tatgaataaa tctccaaatg gttttagtat tgaagctttt ggaccaagta
    13861 ctaatcctac tatcacccct attatagtac atgctaaaag tataattgat gacttataat
    13921 tttttaaaaa gtccctcatt tatattttcc tttctaatgt tttatattat aaattaccct
    13981 aaatcctaaa aaaattaaag taaattatct aatgcgcatt aataattttt gtaattttcc
    14041 ccaacag
//
LOCUS       DAELXX010000103        13990 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.103, whole genome shotgun sequence.
ACCESSION   DAELXX010000103 DAELXX010000000
VERSION     DAELXX010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13990
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.103"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            78..578
                     /locus_tag="KPH29_002910"
     CDS             78..578
                     /locus_tag="KPH29_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG1468491.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNTINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSSLL
                     KQIIDLSKEYGYEKIELDVFKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
     gene            complement(828..1607)
                     /locus_tag="KPH29_002911"
     CDS             complement(828..1607)
                     /locus_tag="KPH29_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468492.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKALDKENNVLGKSKALSFEYKNGNDEISLDTANNVLEKS
                     SDNLKVVDTYKDINISYEAFAQKFVPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNEFKFLTWKQDGVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
     gene            complement(1604..2083)
                     /locus_tag="KPH29_002912"
     CDS             complement(1604..2083)
                     /locus_tag="KPH29_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG1468493.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRTLLKTKLENTEVII"
     gene            2889..3590
                     /locus_tag="KPH29_002913"
     CDS             2889..3590
                     /locus_tag="KPH29_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBG1468494.1"
                     /translation="MKFQEMINSPGLWIVSSFLVIISVVQAIVFMKSALKEASNLGIE
                     RKGIKAAIRSASVTAIGPSLSPVITLLSLVAVIGAPTTWMRLCDVGAARTELGVISLT
                     SNLSGVEVGSAAFGAEAFSYALWGMALNNLGWLLVVFILGHRMRGIVEKMNVKYNPTW
                     VKKLLAGATVGLFAYLLSNQIKTFEIPKLTPAVISAIVMLVLTTLFKKNQRLQELSLG
                     IAMLIGMFGTQIVLS"
     gene            3616..4272
                     /locus_tag="KPH29_002914"
     CDS             3616..4272
                     /locus_tag="KPH29_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468495.1"
                     /translation="MYDKKMIKIGRISIGLAIIANFIPAIYVGLRFGQMPMLSVIFQI
                     WGLVATTYGVSWIVQPIAYYPTLGAPGSYIGWLAGSVGDIRMPAASMAQKIAGVEAGT
                     HEGEVVGTIGTCCSIFVSASMITIFTIVGYKVIPLLPEFITDSFNYILPALFGAIYVD
                     IARKDIRAGGCTIAVALAIMYFGEKIGIPGGLLTLFIVLGGILITRVFFVIDSNKTKE
                     "
     gene            4479..6119
                     /locus_tag="KPH29_002915"
     CDS             4479..6119
                     /locus_tag="KPH29_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBG1468496.1"
                     /translation="MFDVKIANGIIIDGTGNSRYKGDVGIVGDKIMAIGDLSQKEAKE
                     TIDATGKFVSPGFIDFHTHSDLSLVYDKYTRSRIHTGVTTDVIANCGIGVAPIREEKK
                     QELIDYLGTRIIGTIPIKLNLHWNTMQEYFDYLTENSPAVNVVAYVAQGPVRINEMGF
                     SKEPATLEQLKNMKLEVRKAMEAGCVALSSGLVYLPGAYTKKEEMAELCKELIPYNGY
                     YISHIRDEGDEEMEALDEAIYIAKTAGVPLHISHLKVMGHKNFGTIDKVFKKLDEAEA
                     DGLEVTFDCYPYTAGMTSLGALLPPWAFEGGVENMVKRLEVQENRDRIIKELEEGIPG
                     WQCFYQLAGGWDGVVLASVMTEANKYVEGKTLMEVAKINGENPFDTFFRLLIEEKSKI
                     QVVVHTQGQEDTDKVVCHPKSCIGSDSMDLSTEGLLSLGKPHPRAFGTFGRIFSYYVR
                     EKGMLTFEEAVKKITYLSAKRLGIYKERGLLKENYFADIVVFDPDTIEDKATYSNPKQ
                     YTVGVEYVLVNGKIALAGGKQTDVCAGRVIKNPLSIAK"
     gene            6478..7800
                     /locus_tag="KPH29_002916"
     CDS             6478..7800
                     /locus_tag="KPH29_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468497.1"
                     /translation="MKILYVGPGMVIDKILAVSKKNFPNIEADFLRYDRYIEVPDLLE
                     KYRGRVDAVLFTGKSPFKFFEKRQKDFVLSDYIPRHETTLYRVLLEITYLLKFDISRL
                     SIDTYDKAMLKRLYKEIGVEFKDEHMFFAEQKYLEEDYIKFVLEFHKYNYQNNDICCC
                     ITGLEEAYHAMKKENIPVVLALPSEDVMVQSIKNIQMRYIAKKNNENQIVVLAIKLNM
                     PSEYSLMKEDEYAYLSQRIKILDKLYHFNSRIDGVLVEQSRSEFMIFTTKKILELETK
                     KYRDIYLLDMIREVSVINTYIGIGYGKTANEAKFNAYESIKMAQSQKNDAAYVVFENG
                     EIMGPLKPNPAVKEKNSFDERFYQIATETGLSANTVYKVFNKIINSGKTEFTSKELAI
                     ICGVSIRTMDRIILRLCDSGYCEVVSEQLMSKYGRPSRILRFKPFLLF"
     gene            7997..9004
                     /locus_tag="KPH29_002917"
     CDS             7997..9004
                     /locus_tag="KPH29_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBG1468498.1"
                     /translation="MGKYQDSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDK
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGTMGCPPEILKKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            9042..10439
                     /locus_tag="KPH29_002918"
     CDS             9042..10439
                     /locus_tag="KPH29_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HBG1468499.1"
                     /translation="MASTNKNNVTSKEEEKEVSTSGLESCTVKENKAKMNQTNFSNIN
                     ENQTNENKTNTYKGNIDKINTNKSDINKVNIDTVDTDKLNTNTLTEKNKKVIFKGEDN
                     MSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDYIMKITHNIWE
                     EKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIGQNVIWSNFGA
                     DGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEWLVRDNLWIVT
                     QLGLNPQEVAKSMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDDSVGEMMLEMT
                     SRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLLASIPNGSYEVERVT
                     CNQREKNEGYDVSIRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQGKVKEEWITF
                     DGMDVLKQMYMGVEE"
     gene            10584..11849
                     /locus_tag="KPH29_002919"
     CDS             10584..11849
                     /locus_tag="KPH29_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG1468500.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTDIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTGIGMSAAVAG
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            11891..12930
                     /locus_tag="KPH29_002920"
                     /pseudo
     CDS             11891..12930
                     /locus_tag="KPH29_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
     gene            12965..13987
                     /locus_tag="KPH29_002921"
     CDS             12965..13987
                     /locus_tag="KPH29_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG1468501.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMVAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
BASE COUNT         5019 a         1612 c         2635 g         4724 t
ORIGIN      
        1 atttatatag cattataaac tagaatcata taaaataagt taactacaat cataaattaa
       61 aaaggagaaa tgttaagttg atagaaggaa taaaatttat aaaggcagaa gaaaagtata
      121 tttattcgta ttggcaaact tttgataaaa tagcaaaaga gaggaaatat ctagctatgg
      181 atgaagcctt tccttttgaa gaaacagttg aatttataaa aaatactata aataaaaact
      241 taccacaact gtttataatt gatttagaat cagataactg tattggttgg tgtgacgttt
      301 tacctaaaac tgaaaaagtt ggttatttag gcatgggtat tcttaaagag tatagggaaa
      361 aaggtatcgg aagtagtctt ttaaaacaaa ttattgattt atcaaaagaa tatgggtatg
      421 aaaaaattga gcttgatgta tttaaaagta atagtagagc tattcatgtt tataaatctt
      481 tagggtttgt agaggtaaat acaatatcta gtggttttac atggaatgat agacctgtaa
      541 aagaagaagt aattcaaatg gagcttacac ttatataaaa taaagttaaa gtctttaaaa
      601 ttccatatac acttgtataa aataaagtta aagcccttaa aattccatat aaaagaaata
      661 tttttaaggg ctttttaata atggttcttt tgaaggtagc taaatatcta aaattttata
      721 tattaaattt aaagaaaata taaagatgga agatacaacc atgtttacta ataaaaaaat
      781 taaagtcata aataataaac tcataaacaa cttattatta cttatgttta ttttgcatct
      841 attacttcat gagccatttt aactaagtca ttcatagata aatttgagtc ttgaactgta
      901 aaagaatagt aaactccatc ttgtttccat gtcaaaaact taaattcatt aacttcaact
      961 ttgtctgatt cattatttgg ccctacacta aatgtgtatt tgcctgattt ttcatcaatt
     1021 ttatcctgtt ctgtcatttt ataaccttct ggtacaaact tttgtgcgaa tgcttcataa
     1081 gaaatattaa tatctttgta agtatctaca acttttagat tgtcagaact cttctcaagt
     1141 acattatttg ctgtgtcaag agatatttca tcattgccat ttttatattc aaaactaagt
     1201 gcttttgatt ttcctaaaac attattttct ttgtcaagtg cttttttatt tacagtatat
     1261 gctcctttaa atttatatcc attactaaat tcagaaacta tattaggggt aaagttaaag
     1321 tcttttttta cttgttctgt agttgggata gatgtatagg tagccttagt acttgatttt
     1381 ccaactaaac tatatatatg tcctactgca agggctgttg tgctcaatat caaagtaatg
     1441 gctaaagttg caactaagcc ttttctaaat ggtaatttgt gtgttttatt attaatcatt
     1501 ttataatttt cctctctttc tggtaaacta tttagtgttt tacttaaact attatgaaaa
     1561 ctttctggaa cttctggaaa cgaattattt aatatatttt tattcatatg attacctccg
     1621 tgttttctaa tttagttttt aataatgttc ttcctcttga taatctactt tttacagttc
     1681 cttgaggtat atctaatatt tccttaacct catctactga ataaccttca atatagtaaa
     1741 ggactattgg aattttaatt tttttaggta gactttgaat agcagtatac aaatcgctat
     1801 agtcatcttt ttcatcaatt tttttgtttt tgaagacatc ttcaaatgat agagatttta
     1861 atcgtttgcg tcttaatgaa ttacattcat ttatgactat tctaatcagc catgttttaa
     1921 agtattgttc ttccttaaga gaatccaact tttcatatgc ttttaaaata gcattgttga
     1981 cggcatcttc acagtcttgg tcgtttccta gaatagactt agatactcga tacaaagtct
     2041 gttctgattc aagtatattg tttgtaaatg tagttttatc caactgagtt ccttcccttc
     2101 tatgcaaatc gtagcgctac ttttttgcct ttcacttatt agataaaatt ttactataga
     2161 tggttcactt tttttatgat tatttgataa atttaagttt gaaagttttg cctattctat
     2221 acaaatattc tataatatat ttttttctag tattttcccc tcatatataa agccatgtag
     2281 atatattaaa ctttagaagc catttatata tttagtttta tagtaagtgt ttatattaag
     2341 taattataaa atagtaagta tttgatgttg agtagttaga aaataatgct tttggattat
     2401 ttacttcatg tatagattta aaataagttt tttaattaat gactaaagat aacggtattt
     2461 tgactaaaag taggctttta agaaaaaaca atgaaactta aatgagaatc aagagttaga
     2521 aatgtcatta ttttagatag atatgaacat aaataaactt aaaaaatata aattataaaa
     2581 ataataattt ttttaaaata acaaagtttt ttgattaaaa gttttatatt gaaatgtcaa
     2641 agtaataaaa taccttataa gttaattaca ggttaaaaaa ataatatgtg aattttctga
     2701 tatttactaa ttatcaataa agtatttaag tattaatgtt tttagtgaga ctttaagaca
     2761 gataatctcc tattaaagat actatatctt agataaaata aattaaaaca atataaaaac
     2821 ataacaagat tacagaaaga aagctcataa taaaacacta aaaaatttat tttaaggagg
     2881 aataaagtat gaagtttcaa gaaatgatta atagcccagg tctttggatt gtttctagtt
     2941 ttttagtaat tatttcagtt gttcaagcaa tcgtatttat gaaatcagca cttaaagagg
     3001 cttcaaatct tggtattgaa agaaaaggaa ttaaagcagc aattcgctca gctagtgtta
     3061 cagcaattgg accctctcta tctcctgtta ttacattatt atcactggta gctgtgattg
     3121 gtgctcctac gacttggatg aggctttgtg atgtaggagc tgccagaaca gaacttggag
     3181 taatatctct tacatcaaat ttatctggag tagaagtagg tagtgctgca tttggagcag
     3241 aagctttttc gtatgcacta tggggaatgg ctttaaataa cttaggttgg ttgcttgttg
     3301 tatttatttt gggtcataga atgcgtggaa ttgttgaaaa aatgaatgtg aaatataatc
     3361 ctacttgggt taaaaaacta cttgcagggg ctacagttgg attatttgct taccttcttt
     3421 caaatcaaat aaaaacattc gaaataccaa aacttacacc agcagttatt tcagctatag
     3481 ttatgctagt attgacgact ttatttaaaa agaatcaacg attacaagaa ttatccttag
     3541 gtattgccat gttgattggt atgtttggca cacagattgt actatcatga ttaaaacaga
     3601 taagaggtga taattatgta tgataaaaag atgataaaaa tagggagaat ttctataggt
     3661 cttgcaatta ttgcaaattt tattcctgca atctatgtag gtcttcgctt tggtcagatg
     3721 ccaatgcttt ctgttatatt ccaaatttgg ggattagttg ctacaactta tggagtgagt
     3781 tggattgttc aacctatagc ttattatcca acactaggag ctccaggttc ttatataggt
     3841 tggcttgcag gttctgttgg agatatacgt atgcctgcag catcaatggc acagaaaatt
     3901 gcaggtgtag aagcaggaac tcatgagggt gaagttgtag gaacaattgg tacatgttgt
     3961 tcaatctttg tatcagcttc tatgattact atttttacca ttgtaggcta taaagtaatt
     4021 cctttgttac ctgaatttat tactgattca tttaattata tacttccagc attgtttggg
     4081 gcaatttacg ttgatattgc gcgtaaagat attcgagctg gtggatgtac gattgctgta
     4141 gcattagcta ttatgtactt tggtgaaaaa attggtatac caggtggatt gttgacattg
     4201 tttattgtgt taggtgggat acttattaca agggttttct ttgtaattga tagcaataaa
     4261 acgaaagaat aggattgttt actaggaaga aagactagaa ttatttgctg agaaaaagaa
     4321 taggattgtt tactaggagg aaagactaga attatttgct gagaaaaaga ataggattgt
     4381 ttactaggag gaaagactag aattatttgt tgagaaaaaa gaataggatt gtttattaag
     4441 aaatatgaat ataaataagt cttgaaagga gaagaagaat gtttgatgtt aaaattgcca
     4501 atggaataat cattgatggt acaggaaatt caagatataa aggtgatgta gggattgtag
     4561 gagacaaaat catggctatt ggagatctaa gtcaaaaaga ggctaaagag actatagacg
     4621 caactggaaa atttgtatca cctggtttta tagatttcca cactcattct gatttatctc
     4681 ttgtgtatga caaatacaca agaagccgta tccatacagg tgtaacaaca gacgttattg
     4741 caaactgtgg tataggagtt gctccgataa gagaagagaa gaagcaggag ttaattgact
     4801 atcttggaac gaggataatt ggtacaattc caataaaatt gaaccttcac tggaatacaa
     4861 tgcaggaata ctttgattac ttaactgaaa attctccagc agtaaatgtt gtagcttatg
     4921 tagcacaagg acctgtacgt atcaatgaaa tgggattttc aaaggagcca gctactttag
     4981 agcaacttaa aaatatgaag ttggaagtta gaaaggctat ggaagctgga tgtgtagctc
     5041 tttcgtcagg attagtttat ttaccaggag catatacaaa gaaagaagaa atggctgaat
     5101 tatgtaagga attgatacca tacaatggat actatatatc tcatattaga gatgagggtg
     5161 atgaagaaat ggaagctctt gatgaagcaa tttatatagc taagacggca ggagttcctc
     5221 tacatatttc tcatttaaaa gtcatgggtc ataagaattt tggaactatt gataaggtat
     5281 ttaaaaagct agatgaagct gaggcagatg gcttggaagt tacttttgat tgttacccat
     5341 atacagctgg aatgacatct ttgggtgcac ttttacctcc ttgggctttt gaaggtggtg
     5401 tagaaaacat ggtaaaacga ttagaagtgc aagaaaatag ggacagaatt ataaaagagc
     5461 tagaggaagg gattcctgga tggcaatgtt tctaccagtt agcaggtggt tgggacggtg
     5521 ttgtactagc ctctgtaatg actgaagcaa ataaatatgt agagggtaaa actttaatgg
     5581 aggtagctaa aatcaatggt gaaaatccat ttgatacgtt ctttaggctt ttgattgaag
     5641 aaaaaagtaa aattcaagta gttgttcata cacaaggtca agaggataca gataaggtcg
     5701 tttgccatcc aaagtcatgt ataggttctg atagtatgga cctttctaca gaaggcttac
     5761 tttctcttgg aaagcctcat ccccgtgcat ttggtacttt tggacgaatt ttctcttact
     5821 atgttagaga aaaaggtatg ttgacttttg aggaagcagt taaaaagata acttatcttt
     5881 cagcaaaacg cttaggaatt tataaagaaa ggggtctact taaggaaaat tattttgctg
     5941 acattgtggt atttgaccca gatacaatag aagataaagc gacttatagt aatcctaaac
     6001 agtatacagt gggagtggaa tatgtattgg taaatggtaa aattgcattg gcaggaggaa
     6061 agcaaactga tgtatgtgct ggacgtgtaa ttaaaaatcc tcttagcata gcaaaataga
     6121 atacattaag aacttttata aaaatagtct ttctttgaat attaaggaag gctatttttg
     6181 ttaaattaat aaagattact ctacttgtgt tttctagaga atttaacttt aagtagaaaa
     6241 aacaatatat taattgtaaa atgtaattaa tattaatgga taagatttaa atacaaaaac
     6301 ttaaatacaa aaatgtttta acagatagtc ataattaaaa aacttaaagt aactttttat
     6361 ataagatata attgattatg caaaatttta tgaagcagca accttgttac tttaagtcaa
     6421 gggaggttca gaaagtgtct taagagataa tagtatcttt aggaaaggag aaacgaaatg
     6481 aaaatacttt atgtaggacc aggcatggta atcgacaaaa tactagcagt aagtaaaaag
     6541 aattttccca atattgaagc agattttctt agatatgata gatatattga agtacctgat
     6601 ttattggaaa aatatagagg tagagtagat gcagttcttt ttacaggtaa atcgccattt
     6661 aaattttttg aaaaaagaca aaaagatttt gtgttgtctg attatattcc tagacatgaa
     6721 acaacattat acagagtatt gcttgaaatt acatatttgc ttaaattcga tattagtaga
     6781 cttagtatag atacttatga taaagctatg ctgaagagat tgtataaaga aattggagtt
     6841 gaatttaagg atgaacatat gttttttgca gagcaaaagt atttagaaga ggactacata
     6901 aagtttgtgc ttgaatttca taaatataat tatcaaaata atgacatatg ttgttgtatt
     6961 acaggtcttg aagaagcata tcatgcaatg aaaaaagaga atattccagt agttttagcc
     7021 ttgccttctg aggatgtaat ggtacaaagt ataaaaaaca ttcagatgcg ttatattgca
     7081 aagaaaaaca atgagaatca gattgttgtt cttgctatca aacttaatat gccttcagaa
     7141 tattcactga tgaaggaaga tgagtacgct tatctttcac aaaggattaa gattcttgat
     7201 aaactatacc atttcaatag ccgtatagat ggtgttttgg tagagcagag tagaagcgaa
     7261 tttatgattt ttacaactaa aaaaatactt gagttagaaa caaagaagta cagagatatt
     7321 tatttgttgg atatgattcg tgaagtttca gttataaaca cttatatagg aattggttat
     7381 ggaaaaacag caaatgaagc taaatttaac gcttatgaga gtattaagat ggctcaaagt
     7441 caaaaaaatg atgcagcata tgtagttttt gaaaatggag aaattatggg accactaaag
     7501 ccaaatcctg ctgtcaaaga gaaaaatagc tttgatgaac gattttatca aattgctaca
     7561 gagacagggc taagtgctaa tactgtatat aaagtgttca ataaaataat aaatagtgga
     7621 aaaactgaat ttacatcaaa ggaattagct attatatgtg gtgtaagtat tagaactatg
     7681 gacaggatta tacttagatt gtgtgattct ggttattgtg aggtagtgag tgaacagctt
     7741 atgagcaagt atggtagacc cagtagaata ttacgtttta aaccttttct gttgttttaa
     7801 atagatataa gttaaaaatt aaatttttat gataacattt tctttacaat tttttagata
     7861 tatgttaata tttagaaaaa tgcatatgta ttcataaaat aaactcaaaa tagctaaaaa
     7921 atcattaaaa aggaaatcgt atgcataaaa tgagaaagtt aatttataac ttaaataaat
     7981 ctgggaggag ataattatgg gaaaatatca ggattcaaaa aaagttgtaa gaaattattt
     8041 tgaagcatta gaaaatgcta ctggggatga ggttgaaaat gtcttaaagc aacatatgaa
     8101 atcagattat aactggaaag gtgtgtatcc atttcgtgaa caagaaggca ctgaaaatgt
     8161 agcagatgta ttttggaagc ctttaaaaaa atcattgagt aatatgcaaa gacgacaaga
     8221 tattttcatt gcaggaacaa atgagattga caatagtgaa tgggtaatga gtatggggca
     8281 ttttatggga ctttttgata aggattggct tggcattaaa catactcgta aaatgattag
     8341 tttaagatat gcagaattta actgtgttca agatggtaaa attgctaaaa ctggattatt
     8401 tgtagatata attgggttta tgattcaagc aggagttaat ccattgccac cacaaacagg
     8461 ttcatatttt gtatatccag ggccaattga ccataatgga ttactttttg aagatgcaga
     8521 tgaagttgaa ggagtaaaaa ctttagcatt agtaaataaa atggtagatg atttatctga
     8581 attaaatgaa agtggtacta tgggatgccc accagaaata cttaaaaaat cttgggctaa
     8641 agatatgatt tggtatggtc caggtggaat tggtgcttct tatactatac ctagatatca
     8701 agagcaacac cagctaccat ttagaaataa tttaaaagga aagactttca atgggcatgt
     8761 atgtagattt gcagaaggta attttgcttg tttctttgga tggcctaatc tgactaatgt
     8821 accatgtgga ggttttttag gattaccagg tggagaggtt aaagcagata tgcaagttgt
     8881 tgatgtttat tatcgtaagg gcgataaact tcaagaaaac tgggtgttaa ttgatattcc
     8941 ttattggttg aaacaacaag ggcttgatat tcttgagaga actaaaagta tttttaatgc
     9001 ttagttatgc atacgtattc taaaacaaag ggggatataa agtggcatct accaacaaaa
     9061 ataatgtaac aagtaaagag gaagaaaaag aagtttctac aagtgggctt gaatcatgta
     9121 cggttaaaga aaacaaagct aaaatgaatc aaactaattt tagtaatata aatgaaaatc
     9181 aaacaaatga gaataaaacg aacacatata aaggaaatat agataaaata aacacaaata
     9241 aatccgacat aaataaagtt aacatagata cagtggatac agataaatta aatacaaata
     9301 ctcttactga aaaaaataaa aaagttatat ttaaggggga agataatatg tcatctacaa
     9361 aaatttacta tggagattca ttagaagtaa cgcctgttgg agtaatggat tacaatgatt
     9421 tttcaaaaca gacaaaaagg gaacaggaaa tacctggatt tgattctaag tatagagatt
     9481 ttgtagatta tattatgaag attacacata atatctggga agaaaaaggt ataggtgtta
     9541 tttatgatac atatcacaat aatgttacaa tgcattgtgg ctcttcaaat ttagttggta
     9601 ttaaagatgt tatttccaat actcttcaaa cattacatgc gtttcctgat agaagattaa
     9661 ttggtcaaaa tgtaatatgg tctaactttg gtgctgatgg ttttttatct tctcaccgtg
     9721 ttttgtctac agcaactaac ttaggtgata gtaattttgg accagcaaca ggtagaaaaa
     9781 taaactttag aactgtaatt gattgtgcca caactaacaa tagaatccac gaagaatggt
     9841 tggttagaga taatttatgg attgttactc aattaggatt aaaccctcag gaagtagcaa
     9901 agagtatggc aaaggcaagt gaatctaaag tgcttagctt acaatcaact tatggtatct
     9961 gtgaatctat ggatggtcaa tttatgccaa ctaagtatca agcaaaagat gattctgttg
    10021 gcgaaatgat gcttgaaatg acaagccgta tttataatta taaatatata aatgaagtta
    10081 aaaaatacta tcatgataat gcagtagtac attttatatg tgataaagat ttaaatggtt
    10141 atgatgagat tcaaggaatg attgtaagtt tacttgcatc aattccaaat ggaagttatg
    10201 aagtggaaag agtaacttgt aatcaaagag aaaaaaatga aggttatgat gtatctataa
    10261 gatggagatt acgtggaata aatgaaggta taggattttt tggacagcct tctggaaagc
    10321 atatagaagt aatgggaatc aatcattatc atatacttca aggtaaagta aaggaagagt
    10381 ggataacttt tgatggtatg gatgtattaa aacaaatgta tatgggagta gaagaataaa
    10441 gctgtttaga gtataaataa ttttgaaatg tatataggga agtattgctt tataaaattt
    10501 aagtggggaa tgtatatgaa aaatagatgt ttaaactttt agcaaaaatt atgactaaat
    10561 ttaaaagggg gaattataaa aatatgaaaa caacggttga aatagaaaat gatatcatat
    10621 taggaaaaga ctattcaaac ctaaaaagag gaattgcatt tttttcaatt gctttaatat
    10681 acttttttta ttgctacaat tttatggtgg gtacttttat taaaccaaca atgatttatg
    10741 ctttagcaga tgggggattt ggcttttcat taaaacaaac agaagaaata tttgcagtta
    10801 tgtcttttgg tacaattcca ggtacattta tttttggggt gatttctaca aaaattggga
    10861 agaagaggac tttaattagc gttgctttac tgattggatt gacaacattt ataccaatgt
    10921 tatctcctac agatattatg ttatggaaaa tagcacgttt gtgtacagga gtagtgttgg
    10981 gaggcgtatt tggaacagct atgcctttag ttgcagatat gtttccaagt aaatatcgtg
    11041 gtaagttagc tgctatatta actgctttat tttcattagc aatgattttt ggtgggaaag
    11101 tatatggcgt tttaggtgat gcaaattggc aaatattgat gtatactgcg attataccac
    11161 ctatagttgg agctatctta gcaatcttct ttgtgccaga tgatttggag tatacaaagg
    11221 aattagttaa aaagggaaaa gaaactggtg aaaagattag ctatataagt atgtataagg
    11281 gaaaatactt atggattgga ttgggaacga ttcttttatc aggtgcaaat tttacagcat
    11341 attcagcatt ttcaaataat gcaactacat acctagtgac aggaatagga atgtcagcag
    11401 cagtggctgg gtctatatat agtttacaag gaattggtca attagtagga tatttattct
    11461 ggggttcaat tgcagataaa tttggaagaa aaataccttt tataggtatg gcattagcag
    11521 cagtatttgt ttttatatat acaaaattag gtggagataa tacaacaaca ttctatatga
    11581 tatcagtatt actaggtgtt gctgttggat attctggagc atggggagca tactacacag
    11641 agttgtttcc aagtaaatat cgttcgttgt catctggtat gtcatttaat ggaggaagga
    11701 ttatatctac gtttgcaata cctgctatag ctggtacagc ttctggtact ttaggtatga
    11761 tgactatctt ttacatatca atcgcagtct ttgtagcagg tgctattgta tggttattct
    11821 taccagagac gttaaataat aaaaaataaa atactaggaa tttattttgt tacttagaaa
    11881 ggggtggctt atgatttcat caattgatgt atgtattata ataggatatt tgctattaat
    11941 gttagttatt ggttattatt caggaaaaga taataaaaat caagaagatt atttcttggc
    12001 aggtaggtca atgccatgga ttccaattgc tttatcagtt gcagctacta tgattagtgc
    12061 aaatggcttt ataggtggtc ctggatgggc atatgtagat ggaatgtatc cagtaatgta
    12121 aaatgtgcaa aaaattcttt tgtagttagt gcatttgtta tgaatttagt ttactacttt
    12181 atgctattgg taggagtaat tttatttgtt ttttatgggg gaaggacatt tgaaacttca
    12241 aatgaaataa tgattacttt tattttaaat gaacttccag taggagccat tggaattatt
    12301 attgcaggtg tgtttgcagc agcaatgtct agtatcgact ctatattaaa cagtatgaca
    12361 actgttttta cgaaggatat ttatgagttt tatttcaaaa aagacaacaa agaatcatca
    12421 ttaaaagtta caatgattat aactgtagtt ataggagttg ttatgacagg ttttattatt
    12481 atgggatttg gaggaagcat caaatcaatt cttgaccttg ttggtaacta tatctcatac
    12541 tttgctggac cagctacagg tgcatttgta ctagcaatgt ttacatataa agctaatgat
    12601 aaaggagtat ctattggttt tattgtaggg ttagttttag gattttttat ttctaataaa
    12661 tataatgtga gttggttgtg gaatccagca ataggtgcta caataacatt gatatttgga
    12721 tatattttta gtgtcatttt aaagaaaaat cataaagtag atgatattaa aaaatacact
    12781 gcatttggta tgagaaatca ccttatttct caaggaatgg ataaagtaga tggagtatct
    12841 atagtgccat tttctttagg taaacaagaa ctaattgtat tagctttttt tattatccaa
    12901 tatattatat tatttttgat acaattttaa gaagaaatac tagatattta aggagaaata
    12961 ctaaatgaat caaaagaaag ttcattataa taaatcggct acatcaaaag atgtcgcaag
    13021 attggcagga gtatctcaat catctgtttc aagagcattt ggtagtgcaa atggaaaggg
    13081 cgtaaaacct gaagtaagag aaagaatttt tcgtgtagca aacgaaatag ggtatgttcc
    13141 taatttggta gcacggggaa tgattagtgg aaaaactaat gttataggtt tgatagttgg
    13201 tgatagtcta ggtccatttt acaatagaat tatcaattta tttgtagaaa aaattcaaga
    13261 gataggtaaa cagtgtctcg tttttaaagt tcctagacaa caaagtatag atagtataat
    13321 ttcaaaagta attcagtttc aagtagaagc tgtgattatt acagcttcgg ccatgaataa
    13381 ggtgatggct gagacttgtg aagaaaacaa tattcctgtg attcttttta atcgttttat
    13441 acctggtata aaaataagta cagtctatgt agacccaatt gaaggtgggg gcatggttgc
    13501 tgaatatctg cttaagaaag gtcataagaa tataggatat attcaattta cgagagaaac
    13561 aagtgaagaa atggaaaaga aaattggatt ttattctaag ttaagacaaa gtgggattca
    13621 taaccttaaa gaagaatctg caaactatga ttatgattct ggatatgaag caggtaagag
    13681 aatgttagca ttatcaaatc ctccaacagc aattttttgc acaagtgact tgatagctat
    13741 tggtgtaatg gatgctgcaa ggtttgagta caagttgagg gtgccagagg atttatcagt
    13801 gataggatat gatgatattc aaatggcttc atggaagagt tataatttaa cgacgattag
    13861 acaaccacta gatttgttga ttgaaaaaac aattaacata cttaagtcct tattaaatga
    13921 agaaaatcca gagtcagtcg ttgaaatgtt aaaacctgaa ttgattgaaa gaggttctac
    13981 actttaagtg
//
LOCUS       DAELXX010000104        13862 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.104, whole genome shotgun sequence.
ACCESSION   DAELXX010000104 DAELXX010000000
VERSION     DAELXX010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13862
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.104"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..557)
                     /locus_tag="KPH29_002922"
     CDS             complement(<1..557)
                     /locus_tag="KPH29_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HBG1468502.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIA"
     gene            complement(965..1342)
                     /locus_tag="KPH29_002923"
     CDS             complement(965..1342)
                     /locus_tag="KPH29_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBG1468503.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            2193..2678
                     /locus_tag="KPH29_002924"
     CDS             2193..2678
                     /locus_tag="KPH29_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468504.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFDLDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            complement(2878..3417)
                     /locus_tag="KPH29_002925"
     CDS             complement(2878..3417)
                     /locus_tag="KPH29_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG1468505.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEASCSQTDDMGEILEKMIGADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVAAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(3410..3823)
                     /locus_tag="KPH29_002926"
     CDS             complement(3410..3823)
                     /locus_tag="KPH29_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG1468506.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEAVSYE"
     gene            complement(3859..4980)
                     /locus_tag="KPH29_002927"
     CDS             complement(3859..4980)
                     /locus_tag="KPH29_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBG1468507.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILVPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
     gene            5579..7231
                     /locus_tag="KPH29_002928"
     CDS             5579..7231
                     /locus_tag="KPH29_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HBG1468508.1"
                     /translation="MNNKNLAILISGMMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYKMSTNVLKEIDKIKKVENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSDILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAVPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKIEYEITINAITGKILEFEIDD"
     gene            7528..8277
                     /locus_tag="KPH29_002929"
     CDS             7528..8277
                     /locus_tag="KPH29_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1468509.1"
                     /translation="MNTILKVNNITKTYNKGQQPSLDKVSFNVSEGEFIGIMGASGSG
                     KTTLLNVLSTIDKPTSGDIFINNINLKSLNDTCAADFRKDNLGFIFQEYFLLDSLTIF
                     ENISVPLTLQKLNPDKIENMVKSLAERFDIASQLNKYPNELSGGQRQRASAVRAIVKH
                     PSILFADEPTGALDSNSATELLHSLQEANEELKTTILMVTHDAYAASFANRILIFKDG
                     HIIKELNKQPNNRHTFYELIIQEIAKLDTKR"
     gene            8290..10317
                     /locus_tag="KPH29_002930"
     CDS             8290..10317
                     /locus_tag="KPH29_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG1468510.1"
                     /translation="MNIFYMAIKNLKKNFSFYSLYLLSVSFVITVFFAFTSFSVNKIM
                     LEKISTDGRVESMCNVISIFLMAFVIFYMAYSNRFFLRRRTKELGIYALLGYRKFTIL
                     SLLTFENVLICSGAFITGLVLGALSHKGIVFGITALLNLSIDNSQIPFFNINAINKTI
                     IFIALVVLVLAISNGKFLYKTSLVKLIRHEKSAEKKLKFRKIPAFMGFIMILSGYCLS
                     LDILRGAKSVWVSIGFYQMGLITMIIIVIGTVLFINSFLPYIMQKSKKNKRTFYTDMK
                     IIAIPNFIYRIRSNSKTLIMLTLLSATTLTVSSVMALSLYYPIASVSRIAPSEIEFRV
                     EKNKQIEDVKQIVSQYTSNTDDIIFTQTNIYKVKSSSKDLPFEYEIGTSKNNLSNSSS
                     SKEVISRESGFECISFSDYYTLLKAQNRTKLLKQLTPLRDNECILMKYQPNNDGYSEN
                     GTIYPLLINGKETPVAVRETSLNNAISFANSVGTLIVNDSIYEQIAQNKLPMTSILSI
                     NGKSIKNNENLYSNISDYLDGSPYLQGYSHRINDLLSINSSTFLLIGFLVVLFFIAVG
                     SILYFNNISAISDSTSDYKILQKMGYDNIQIKKIIKKQVLPFFNIPFLLGILDCIFAT
                     LVYKTALMQNLLGNSFSQYIPVIIAIFLTSVVYLIYYILTIKSCCKIVLKN"
     gene            10461..12044
                     /locus_tag="KPH29_002931"
     CDS             10461..12044
                     /locus_tag="KPH29_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG1468511.1"
                     /translation="MTNFNLKILFLGNYILSLLFMGISTSTETYMNIQSIIPLIISTL
                     LLIDITYITSCNVRENNIISLFCGLLALDSWYLLLSFVTTPIGTIIFKILSTVIVYVS
                     IKFIFLFLFQGSGYNFRKITDILLLITCIGAIVGAFISNRTYACMYGIQFVVSIISFL
                     YIIFYHLKRVVFVIKSEKKPISISVFITIIGFLTYYFFTLRISNHIGNFGIYLTVLVF
                     FMSIHGIALKENDSFPISTVFNIKQRAIFLFISILVISLVVLFANGNFSIILIAINVL
                     FMLIFLCNIILEFNLRDEKNKVVKNSKYTFALEKLQQEEQLKTEFSNFLHDDILQNLL
                     SIKNMMSKSYRPEVQDIIYETLNNLNTCIRNQMQDYHPVILKNLTVKENYQNLIESVS
                     SSFPQKNICVSFKCSDTLFLVEPYDILIYRLIKELLTNVYKHSNGSHAWIALALEKNI
                     IKLNINDNGVKNTSFKTPLDITKITFDIIKHKGIISIKEQVENLGGTISISDNIPHGV
                     CVQITIPMKGDGSYKYFVS"
     gene            12016..12636
                     /locus_tag="KPH29_002932"
     CDS             12016..12636
                     /locus_tag="KPH29_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1468512.1"
                     /translation="MVLINILLVDDHMLFAKSLSIALEEYEEIEQFYSTQDIHNLSEM
                     IQDKKIDIVLMDINLRGLSECDGLKVAYNLLETNSTIKIIILTGYDLPVYKHEAKKIG
                     IYGFLNKNISPDSLIDSLLKVYQGVTCFPSENTEVIIEDLTNMEKRILQLICSGKKRK
                     IIADELYISERTLSNHLQHIYDKLDVSSSVEAITKAIKMGYISPVY"
     gene            complement(12824..13750)
                     /locus_tag="KPH29_002933"
     CDS             complement(12824..13750)
                     /locus_tag="KPH29_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBG1468513.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSENS
                     NAEIVTKNSDIFKNIEKYIDATINNNQKTMKYKHRDELFKNSNTNTYSRINQESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENLDEDLTEDSTYKNPESIHEII
                     IRKLNSESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLNEESSLSACNYGDMCYEDMY"
BASE COUNT         4994 a         1970 c         1744 g         5154 t
ORIGIN      
        1 gctatattgt ttatataaga tattttgcta tttatgtcta gtattataac gcctttttca
       61 acattttcaa tgatttctat catcatatcc atttttctac tgctattttt tgacgcttca
      121 tgttcttcta ttttcataga aattaaatcg catatatgat tgacaaattt taaataatta
      181 tctatttttg cacttatttc tactttttta gttttatctg tactaatagc accaattact
      241 cctatagttc gattattata ctttattgga actgctatta ttactttatt taggcatttc
      301 atgtaatggg aacattctat acaaagctga ttatcacata tatccaatac gactaattcc
      361 tgacctgtct gtataacatt gtcgtaaata aatccttctg taacaacgct ttcatttatc
      421 tttgatttat acagacctgt accactaatt cttattaatc tatcatcaac tatttcaata
      481 tctacatcta acacactagc tactgtttca gtgtatttta tcatttcttg ttgaaattct
      541 tttaatttac ttaacatagt ttcccccatt agaatttaat ttaccaaatt atcagtttag
      601 ctatatttta acatatagtc actctttaaa atatatataa aacattactt atttatatat
      661 ttacattaaa acacacaata ttaatttata aaaagtttgt tttatatgat gaccttaatt
      721 acttaattat aagatacaga caataaagta aatcacaata caataataaa ataaaaaaag
      781 ctttcctcat ataacaaata tatttttgac ataaaaatat acattatatg agaaaagctc
      841 aatagttaaa tatttagata gatttttata tagacttttt taatataaac ttttttaatt
      901 gtagactttt tttattagtt tttaattttt attagttttg ttattggttt tattgttata
      961 atacttataa tactgccatt gcttcaattt ctaaaactgc atcttttggt aattgagcaa
     1021 cttgtacgca tgaccttgct ggtggatttt ctttaaaata tgtagcatat acttcattaa
     1081 tatcattaaa atcctttaaa tcttttacga atactgtagt cttaaatatt ttatccatac
     1141 ttgtaccagc ttgttctaat attgctttta cattatctaa agaagctttt gttgcttctt
     1201 ttgggtcaga tactaactcg ccagtcttca tatctattgg aagttgacct gatgtagaaa
     1261 ctatgtttcc aacttgaatt gcttgagaat atggtccaat tgctcctgga gctttctctg
     1321 tagctattac tacttttgac atgacttcac ctcctattat taagttatat ttacaggaaa
     1381 ataaaatttc ctaaacacca atattaatac gaacctttag attaaatttc aaatctaaaa
     1441 cttcaaataa tcaatactta gatgattatg tttatttgtg tcttgttaac ttttattaaa
     1501 gcaattaacg tgccaaactt tatttatata taaaattttg tgaagtcatt caaaatagtt
     1561 acttctgaat tttttcgtaa aaaaatgctt taaaattact tttttatgat gtcaattttt
     1621 ttctcatatc tagaaattta ccaacatgag aattcttgtt ttgagaattt acagattaaa
     1681 gttatttttc aatcttaaat tatcaagtta ttacatatga aagaactata gttgacaatc
     1741 ccactattat aaataataaa acctttaccc ataccttatt taccctagtt atcatagagc
     1801 ctattgaaaa tataacagct aaatataata ataaccaaaa aattatcaat atctaccact
     1861 gttgctgaaa atccaaaaaa ctaccttttg atagtattac ttaacaatct aattaatctc
     1921 ataataaatt tacaacttat aaataaattg ttatattggc cgtatctcaa caactttctc
     1981 ctttcctagt tttaaatctt ttatagaatt gtaccacgtc taagtaaatt ttcatactat
     2041 taagtcttat ttctaataaa tcattagctc tcattctatg cccaataatg taagattgta
     2101 gtaattttat tgattttaat agttttattt aatatactta tattaattat tttcatgtga
     2161 aattattact tatatagggg gattttttta atatgagcaa aagtgaatta acggcagaaa
     2221 caacagaaga aatgttagaa gtactaagtg gtaaagatta tgatattgca tgtcatttac
     2281 atgaacttgg taaatcatta gattgtaaaa ttgaaccaaa aacaggtgct cgttcttaca
     2341 aaatagtata ttcaactaag aaaccaaaac gtagcttatt tactattgaa tgtaatgaaa
     2401 aaaaatggag agttaaagca aatctttttc atctaaatac atataaagat gctgtggagg
     2461 aatgctctaa aactattaaa gatagtataa ctaaaactcg tacttgtaag aaatgtaatt
     2521 caaagtgtat tggaggttct tcgtttgatt tagatggaaa gtcttatctg acttgcatag
     2581 gaagtggtca ttattttgca aatatggaag aaatggattg gaaaaaccta gaaaaattaa
     2641 ttactaaaga aaataatatt atgcaggaat ctgtatagtt ttttaagggt aattattaaa
     2701 atatatgagt aacaacagat acttaaatag gcataaaaaa ataaagggta taataaggat
     2761 atagaataaa aataaatgat atagcacaaa gaggctgtct caaaatatag ataagcctct
     2821 ttttattgtt gaaatgataa gaatatattc gattttatac aattttatac aaacatacta
     2881 agccaactta cccatttcat atgcctgttt cattgcctta cttcccttaa tatcacctac
     2941 attccaagca cctacaccat atactatacc tttttcttta gaaccattta gacatgatgt
     3001 aaatcctcta aatccttcta ttgttctttc catatcagcc acattttcat ctgctgctgc
     3061 aacgataaaa tagaaatctt tatcacaaat ttcagtatat ctagagcaag ttctatcaat
     3121 catagttttc atttgcgcat tcatagtata aaaataaacg ggagtcgcta aaacaatcac
     3181 atctgcccca atcatttttt caagaatttc tcccatgtca tctgtctgtg aacaacttgc
     3241 ttctttttca taacaagcac cacatccagt acaatagtta atatctttat cttttaaaaa
     3301 tatcttctcc acttgatttt tagattctaa taccccttgt ataaactggt cacacaataa
     3361 gtcagaattt ccaccttttc taggactagc tgatactatt aatacatttt tactcataag
     3421 atactgcctc cattttctta tttctactgc tctttaatac tttttctttc tcaataatac
     3481 cttcttcata tctatcaatc ttaaaattta atcgttccaa agttttttgc atttcttcta
     3541 ttctttttgc tagttggttt ctctgctcta ttaaaagttc ttttctagct ttgatagtac
     3601 tgctcccttg ttgaaacata gctacatact caattaatgt ctcaatagaa agacctgcac
     3661 ctctcataca ctttatgaaa ttaacccact cacaatcttc ttctgtaaaa gacctaattc
     3721 cacttttatt tctatttaca ggtggtatta aaccaattcg ttcataatat cgtaaagtat
     3781 ccgctgaaag ctcgtatttt ttactaactt ctgaaatcgt catacatatc ttctcctctt
     3841 gatattgtat agattgaatt atttctcaaa ggtttctgct acattcttta aagcatctat
     3901 aattttaatg tgttgaggac agctttcttc acattgttta cattcaatac aatctgaggc
     3961 ctttccataa gttttagtat aattattata gtaaaccatc tgtgttgaaa atccagtatt
     4021 aagagcttgt ttttctgcat tgtacagcgc aaaatagttt ggaatagcga tattttttgg
     4081 acagccttct acacaatact gacaggcagt acaaggaact aaaattgatt cgttgataat
     4141 ctctactgct tcatctataa tattatattc ctcttgtacg aatggtttga aattttgcat
     4201 atatcctgta ttgtctaata attgttccat atttgacatc ccacttaaga ccatcataac
     4261 attatcttta cttgctgcaa aacgtattgc ccaagatgaa attgacatat ctggattgta
     4321 gtcattcaat aatttctctg ctttttctgg tatctttgca agtgtacctc cttttactgg
     4381 ctccataaca attacaggct tattatgctt ctctgccacc tcgtagcatt ttctagattg
     4441 tatgctttca ttatcccaat ctaaataatt aatctgtaat tgcacgaagt ctacttctgg
     4501 atgctcactc aagactttat ccaatagttc tgcactatca tgaaaagaaa aaccaatatt
     4561 ctttattttt ccttcttttt tcttttcttc aataaactta aagctatcaa attttttagc
     4621 tacttcataa tgtgacacgc ctatattgtg taaaagatag tagtcaaaat agtctacacc
     4681 acatttttct aattgctcat taaaaatggt ttcttgctct tcttttttct ttaaagccat
     4741 taatggaagt tttgtagcta tagtaaaact ctctcttttg tgtcttttta ctagagattc
     4801 tctaagtgct atctcacttt ttcccatgtg atacacatag gctgtatcaa aatatgtaaa
     4861 tcccctttct aaaaaagtgt ctaccatttt atttaaatgt tccatatcaa tagttgtaac
     4921 atcattttca tcttttattg gtaaacgcat taatccaaat cctaattttt ttgcctccat
     4981 tgttttactt ccccctttaa aacttgtttg ttttttctct ctactttaac tattcttaca
     5041 agtatataat tcttgtataa ttatacttta ctacttggag ttaacttcaa gtcaatagat
     5101 ttttttattt ttaagacaaa tattataaaa atacagttct ttaatattaa tccattgcat
     5161 gtttctttca ccctccaatt ctcaaacttc acaaactcca acatgcaaag aaatatatac
     5221 tttaagaaat tcagtccatt aatattacta caaatacttt ttagatatgt ttaaaaagaa
     5281 atttcaccta aaatatttat aaaagattta gaaataggtc ttgtgtctat tttaaaatat
     5341 ataaaacagt ggtaaagact tatttataag ataaaaaatg aatagtgtat acccttttta
     5401 aaaagttttt tcctttttat atttgtgata caatgttaaa tataaaaagg aaaattttgt
     5461 attaaagaaa tcttatttgt tgattttgta acttcaaact cagtatcaat ttgattttaa
     5521 gattatattt tattaagtat gctaaataac aaatttttaa agattgtgag gtaaaaatat
     5581 gaataataaa aatttagcta tattaatatc aggtatgatg tttttttcta gttcttcagc
     5641 aatttttgca gacaatacta caaagcaaga aagattaata ggtaaaacaa gatatgaaac
     5701 agcagtagaa gtaagtaaat tgggatgggt tcaatcaaaa acagccatta tagttaatgg
     5761 taattctatt caaagtgcgc tttgtgcaaa tccatttgct aagctaaaga atgcacctat
     5821 acttttagta aacaacaaca gtattgaaaa ttcaactaag gctgagctta aaaggcttgg
     5881 tgtagataat gtgtacattg tagatagtgg aaatagcatt tcttctaaag tagaaaatga
     5941 aataaaatca cttaacataa aaataaataa aatagtagga aacaatatat ataaaatgtc
     6001 tacaaatgta cttaaagaaa tagataagat aaaaaaggta gaaaatgttg cagttgtaaa
     6061 tggactaaag ggattatctg atgcagttag tatagcttct ccagcagcaa tacataatat
     6121 gcctataata ttagtatcag atgaaaacag caatgatagt agtataaatt tcttaaacaa
     6181 taggtctata aaaaaatctt acatagttgg aggaaataag attttatcag acaatacagt
     6241 aaagaaatat ccaaacagag aaagaatcag tggttcagat agaaacaata ctaatgcaaa
     6301 aattattgat aaattttata aagataaaaa aataaataat gtatttgtag ctaaagatgg
     6361 tattaaccaa gaaaatgcat tagtagattc attatcaata ggagtattag ctgctaaaga
     6421 aaattctcct ataatgatat ctaatggtag ccttgtaaat agtcaaaagg aatttattaa
     6481 aaataaagat atagatgaaa taacacaagt aggaggagga tataatcaaa aagcatttga
     6541 agatatttta aaactaaaaa atgtgaacat aagaaataaa actaaaaaaa tatctttaat
     6601 acaggctaaa gaaatattaa aacaagctct tccaggaagc gatattttag tttgttatta
     6661 tgatgaagat gataatgaat atgactgcaa agttataaag gataatatcc tatatgagat
     6721 agaaattgat gcctactctg gtaaaataat agaaattgaa aaagatgatg acttaaatga
     6781 taataattta ggtacacaaa atatatcatt ggataaagct aagtctatag cattagatgc
     6841 tgttcctaat ggtaatttaa tctattgtaa atatgatgct gaagataacg aatatgaggc
     6901 taaagtaata aaaaataaga aaacatatga attagagata agtgcatttg atggaagaat
     6961 tgtagatatt gacatggaca atgattctga tgatgacttt gatgataatg ataagaataa
     7021 ctctaatact aatgacaaca gttctattat tagtgctgaa aaagctaaat caataatgct
     7081 agataaagtg cctggtggga aaatagtaaa atttaagtat gatgaagatg acaaagaata
     7141 tgaaggtgaa ataataaaag gaaaaattga atatgagata acaattaatg caattactgg
     7201 taaaatatta gaatttgaaa tagatgatta ataaaaaata tatattctat taattattac
     7261 aagcaataaa gccataaata ttgaattttt caatatttat ggctttattt tatttttttt
     7321 aatttaaatc tacattatgc cattatatat ctatgcactc ttttaaatta aaatcttgtc
     7381 catcatccca aacctaataa agttagattt taaacaatat attgtgatat aagtaatttg
     7441 tgtagaatta ttgcgtgatt tactcatgaa gaaacaataa ggttaatata taatttgact
     7501 atattaagac gaaaggagac atatacaatg aatacaatat taaaagtaaa taacataact
     7561 aaaacttata ataaagggca acagccgagt ttagataagg tatcctttaa tgtatcagaa
     7621 ggtgaattta taggtataat gggagcttct ggctctggga aaacaactct gcttaatgta
     7681 ttatctacaa ttgataaacc aacaagtgga gatattttca taaataatat aaatttgaaa
     7741 tcgcttaatg acacttgtgc tgctgatttt cgtaaggata atttaggctt tatctttcaa
     7801 gagtattttt tactagatag tcttacaatt ttcgagaata tttcagtacc tcttacactt
     7861 cagaaactca atccagataa aatagaaaac atggtaaaga gtctagctga acgatttgac
     7921 atagcttctc aattaaataa atacccaaat gaattatctg gtgggcaaag gcagagagct
     7981 tcagcagtta gagcaatagt aaaacatcct agtattctct ttgcagatga accaacagga
     8041 gctttggatt ctaattcagc tactgaacta ctccactcac tacaggaagc aaatgaggag
     8101 ttgaaaacaa caattttaat ggtaacacat gatgcctatg cagcaagttt tgcaaataga
     8161 attttgattt ttaaagatgg tcatatcatt aaagaattaa ataaacaacc gaataataga
     8221 catacatttt atgagttgat tattcaagaa attgcaaaat tggatactaa aagataggag
     8281 gataatttaa tgaacatttt ttatatggca ataaagaatc ttaaaaaaaa cttttcattc
     8341 tattctctat atctactttc agtctctttt gttattactg tatttttcgc atttacttca
     8401 ttctctgtta ataagataat gctagaaaaa atttcaactg atggtagagt tgaatctatg
     8461 tgtaatgtta tttcgatatt tttgatggca tttgtcatct tctatatggc atactcaaat
     8521 cgattcttct tgagacgtcg tacaaaagaa ttaggcatat atgctttact tggctatcgt
     8581 aagtttacaa tattatcgct acttaccttt gaaaatgtat taatttgtag tggagctttt
     8641 attactggat tggtattagg agcattgtca cataagggta ttgtctttgg aattacagca
     8701 ctacttaatt tatcaattga taattcacag ataccatttt ttaatataaa tgcaataaac
     8761 aaaacaatta tctttatagc attagttgtt ttagtcttag ctatatcaaa tggtaaattt
     8821 ctttataaga cttcacttgt aaaattgata agacatgaaa aaagtgcaga aaaaaaatta
     8881 aagttcagaa aaattccagc ctttatggga tttattatga ttttaagtgg gtattgttta
     8941 tcactagata ttcttcgtgg agctaaatca gtatgggtaa gtattggatt ttatcaaatg
     9001 ggcttgataa ctatgattat cattgtaatt ggtacagttc tctttatcaa ttcctttctt
     9061 ccatatatta tgcaaaagag taaaaaaaat aaacgtactt tctacacaga tatgaagata
     9121 atagctattc caaactttat ctatcgtata cgttcaaact ctaaaactct aattatgctt
     9181 acattactct ctgctactac tttgacagtt tcaagtgtta tggcactgtc cttatactac
     9241 cctattgcat ctgtttcgcg tattgcacct tcagagatag aatttagagt agaaaaaaat
     9301 aaacaaatag aagatgtaaa acaaattgtc agtcaatata cgtctaatac agatgatatt
     9361 atttttacac agacaaatat ttataaggta aaatcttcat caaaagacct tccatttgaa
     9421 tatgaaattg gtacatctaa gaataattta tctaatagta gttcttctaa agaagttatt
     9481 tcaagagaat caggatttga atgtatatct ttttctgatt attatacttt attaaaggca
     9541 caaaatcgaa caaagctact aaaacaactt acccctttaa gggataatga atgtatttta
     9601 atgaaatatc aaccaaataa tgatggatat agtgaaaatg gaacgattta tccattactt
     9661 attaatggta aagaaacacc tgtagctgta agggaaacat cattaaacaa tgctatttct
     9721 tttgccaata gcgttggaac acttatcgtt aatgatagta tatatgagca aatagcacaa
     9781 aataaattac caatgacaag cattttaagt attaatggta aatcaattaa aaataatgaa
     9841 aatttatata gtaatatttc agattattta gatggaagcc cttatttaca aggatattct
     9901 cacaggataa atgatttatt atcaattaat agttctacat ttctgttaat cggatttctt
     9961 gtggtattat tctttattgc tgttggaagt attttatact ttaacaatat atcagcaatt
    10021 tcagattcaa catctgatta taagatttta cagaaaatgg gttatgataa tatacaaatt
    10081 aaaaaaatta ttaaaaaaca agtattgcca ttttttaata ttccgttttt acttggtata
    10141 ttggactgta tatttgctac cttggtttat aagactgctt tgatgcagaa tttacttgga
    10201 aattcatttt cacaatacat acctgtgatt attgcaattt tcttaacttc tgtagtgtat
    10261 ttgatatatt atatacttac tattaaatcc tgttgtaaaa ttgtattaaa aaattaatat
    10321 ttaccaaatc gtcaagctta gaggtgtgac gatttggtat acttcattta taagtatttg
    10381 atataattta aaattataga ttcttaggtt tgatttagtc ataaaattaa tataattaac
    10441 tcataaggag gtgtataaga ttgacaaatt ttaatttaaa aatactattt ttaggaaact
    10501 acattctatc acttctcttc atgggaatat ctacaagcac tgaaacttat atgaatatcc
    10561 aaagcattat tcccttaata atttcaacat tattactcat agacatcaca tatataacct
    10621 cttgtaatgt ccgagaaaat aacattattt cattattttg tggattacta gcactagaca
    10681 gttggtattt actcttatca tttgtaacca caccaatagg cactattatt tttaaaatat
    10741 taagcacagt tatagtgtat gtctctatta aatttatatt tttatttttg tttcaaggta
    10801 gtggatacaa tttccgtaag ataacagata ttttattgct tatcacctgc attggtgcaa
    10861 ttgtaggagc atttatttct aacagaacgt atgcttgtat gtacggaata cagtttgttg
    10921 ttagtataat aagcttttta tatattattt tttatcattt gaaaagggtt gtttttgtca
    10981 taaaaagcga aaaaaagcct atatcgattt cagtatttat aacaattata ggattcctta
    11041 cttattattt ttttacacta agaatatcaa atcatattgg taattttgga atctatctaa
    11101 ctgtattggt attttttatg agtattcatg gtattgcact taaagaaaat gatagttttc
    11161 ctatttcaac agtttttaac ataaaacaaa gggctatatt tctttttata agtattttag
    11221 taattagctt ggtcgtacta tttgccaatg ggaatttttc tataatatta atagctataa
    11281 atgttttatt tatgcttatt ttcctctgta acattatttt ggagtttaac cttagagatg
    11341 aaaaaaataa agtagttaaa aatagcaagt atacttttgc attagaaaaa ttacaacaag
    11401 aagaacaact aaaaacagag ttttctaatt ttttacatga tgacatatta cagaacctgc
    11461 tttcaataaa aaacatgatg agtaagtcat atcgcccaga agttcaagat attatatatg
    11521 aaacacttaa caatctaaat acatgtataa gaaatcaaat gcaagactat catcctgtta
    11581 ttttaaaaaa tttaacagta aaagaaaatt atcagaattt aattgaaagt gtttcttcat
    11641 cctttccaca aaaaaacatt tgtgtttcat ttaagtgttc tgatacttta ttcttagttg
    11701 aaccatatga tatattgata tatcgactga ttaaagaatt attgacaaac gtgtataaac
    11761 attcaaatgg tagccatgca tggatagctt tagcactaga aaaaaatata ataaaattaa
    11821 atattaatga taatggtgtg aaaaatacat cttttaaaac acctttagat ataactaaaa
    11881 ttacttttga tataataaaa cacaaaggga ttatatcaat taaagaacaa gtagaaaatc
    11941 taggaggaac gataagtatt tctgataata tacctcatgg agtttgtgta cagattacaa
    12001 tccctatgaa aggagatggt tcttataaat attttgttag ttgatgacca tatgcttttt
    12061 gctaaaagct tgtcaattgc actagaagaa tacgaagaaa tcgaacagtt ttattccaca
    12121 caggacatcc ataatctttc agaaatgatt caagataaaa aaatagacat tgtcttaatg
    12181 gatataaacc ttagaggctt aagtgaatgt gatggcttaa aagtagctta taatttatta
    12241 gagactaatt caactattaa aataataatc ttgactggat acgacctccc agtttataaa
    12301 catgaggcta aaaaaatagg tatctatgga ttcctaaata agaatattag ccctgatagt
    12361 ttaattgatt cattattaaa agtctatcaa ggggtaacct gttttccatc tgaaaatact
    12421 gaagtaatta ttgaagattt aactaatatg gagaaaagaa ttttacagct tatctgtagt
    12481 ggtaaaaaaa gaaaaataat tgctgatgaa ttgtatataa gtgaaagaac attatcaaat
    12541 catttacaac acatatatga caagctagat gtttcatcat cagttgaggc tattacaaaa
    12601 gccattaaaa tgggttatat ttctccagta tattaaaaaa gtgagtgtct caaaatgaac
    12661 tttctagctc ataaggcact ctcctttgta ttaaatcaaa tatattttca tcatttaaac
    12721 aattaagaag aggcttctct ctattttgag acaactccat tttaatacac tatttatatt
    12781 tatatataaa catattttaa acaactaaaa agcaactaat atctcaatac atatcttcat
    12841 aacacatatc accataatta caagcagata aagaactctc ctcatttaaa tacgactcaa
    12901 attcataccc aaacagaatt gttggttcta tatatatttc catatctgta tatctccatc
    12961 ttcttacctt tgcctctata accttgtaaa aatcttctaa actataccct tcttttaatc
    13021 ttttatctat aagtgcctta gttatactca aatcagcttt aaaatttttt cctgattcac
    13081 tatttaattt tcttataata atttcatgta tgctttctgg atttttataa gtagaatctt
    13141 cagtcaaatc ttcatccaaa ttttcaaagg tatttttatt taaattcttt ttatttaaat
    13201 tattttttaa taattctttt ttctttgtct cattttctgt ttcataccca gtatgaatta
    13261 tcaaatcata atctttgtct atactctctt gatttatacg gctgtatgtg ttggtattac
    13321 tgttcttgaa caactcatct ctatgtttat atttcattgt tttttggtta ttgttaatag
    13381 ttgcatcaat gtatttctct atatttttga aaatatcact attctttgtt acaatttctg
    13441 cattactatt ttcagaacaa acataactat atacagaaca acaatttccc tttactcccc
    13501 tacttattaa tctcaatata cctaaatcta taaattcatt tacaagtcta cttattgtag
    13561 atatagatac atcaaggtca gatgcagctt tccttactgt catcataaat tgtcctcttg
    13621 gcagagttcc ttttaatctt tgtatatttt cttttctcat tacatagtga ttaaacctta
    13681 ttctatttac atctcttttt tcattaaaat ataatactgc tgaatttaat tgataaaaac
    13741 tattactcat attcccactc ctccaattta aatagataat ttgttgtaaa gtttttagat
    13801 ttttaaataa cttattaaat gattattttt attaaaaagt attctttgat attttcattt
    13861 ta
//
LOCUS       DAELXX010000105        13414 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.105, whole genome shotgun sequence.
ACCESSION   DAELXX010000105 DAELXX010000000
VERSION     DAELXX010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13414
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.105"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1409)
                     /locus_tag="KPH29_002934"
     CDS             complement(<1..1409)
                     /locus_tag="KPH29_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HBG1468514.1"
                     /translation="MKFYKRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGK
                     IADKNAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETSARLKNVSKV
                     YIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVNTVMLTNAY
                     KGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASMSTTLVNNTKSTR
                     LGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGPIVLVNDGSNKSI
                     LKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTIKDGMYKVGKDIS
                     AGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYIKIEDSTMT
                     LAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRNDSLGNAEGIVTN
                     DTFSGRRYITVEEGQYLIL"
     gene            complement(2236..2787)
                     /locus_tag="KPH29_002935"
     CDS             complement(2236..2787)
                     /locus_tag="KPH29_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HBG1468515.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
     misc_binding    complement(2938..3201)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3637..4035)
                     /locus_tag="KPH29_002936"
     CDS             complement(3637..4035)
                     /locus_tag="KPH29_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468516.1"
                     /translation="MNKKKKLIGIAISFLLIISIINPLQVEAEAIIESSVYISSIKEN
                     YTYDNTKFKTIKSKSRHKSRKYNKSKVKLKKRSIRTKKYGYQTINKYKRRNARRHNIL
                     TKLVLSIIVIFLVIVLVVLIFIKRKKKKFY"
     gene            complement(4391..4876)
                     /locus_tag="KPH29_002937"
     CDS             complement(4391..4876)
                     /locus_tag="KPH29_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG1468517.1"
                     /translation="MISELMLKNFGKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
     regulatory      complement(4959..5052)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(5408..5791)
                     /locus_tag="KPH29_002938"
                     /pseudo
     CDS             complement(5408..5791)
                     /locus_tag="KPH29_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      6018..6115
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            6170..6346
                     /locus_tag="KPH29_002939"
     CDS             6170..6346
                     /locus_tag="KPH29_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468518.1"
                     /translation="MFGNKMDKCTHVLTAYISGSYDYCNFLDTQLDDFILEYGESVVE
                     SCLHQVMVLVSKYN"
     gene            6572..6772
                     /locus_tag="KPH29_002940"
     CDS             6572..6772
                     /locus_tag="KPH29_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG1468519.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     gene            complement(6936..7235)
                     /locus_tag="KPH29_002941"
     CDS             complement(6936..7235)
                     /locus_tag="KPH29_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468520.1"
                     /translation="MGFYINYVVCKVKILFSSFSKIILFYINYVVCKLFITLKAILLK
                     TQIIVTTHSPHILQIDSKEEMIVLDMAESGNVYKKELELGEYGVLGWTNEGLYLH"
     gene            7457..7639
                     /locus_tag="KPH29_002942"
     CDS             7457..7639
                     /locus_tag="KPH29_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBG1468521.1"
                     /translation="MFKFYNNATLMFLSGTNIKTISERLGHADIKITMNSYSQFLEEM
                     DREAFENLSKILFIVF"
     gene            7710..8555
                     /locus_tag="KPH29_002943"
     CDS             7710..8555
                     /locus_tag="KPH29_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468522.1"
                     /translation="MNIIKSITLLLTLLIVILMTGCSPRESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISIENLEEFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVV
                     KLEETFNIKNLLYKKDDSLKVNRYFVVENNKYKLLLYESFKKEFGINSMDLAQLKLND
                     FGLKYFKEVDLILKKAIAINPTDSKLYYAQACNYMNFDGFPGYSDTTYNALDSINNSL
                     KYLDKNDSDYKKNASNIYNLKGVILMFHKRFDEAQQYLNKALYFNKDNEDPKYNLNTI
                     KKHLN"
     gene            complement(8779..8943)
                     /locus_tag="KPH29_002944"
     CDS             complement(8779..8943)
                     /locus_tag="KPH29_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468523.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
     gene            complement(9485..9817)
                     /locus_tag="KPH29_002945"
     CDS             complement(9485..9817)
                     /locus_tag="KPH29_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBG1468524.1"
                     /translation="MNTREMKEYRGFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
     gene            complement(10061..10702)
                     /locus_tag="KPH29_002946"
     CDS             complement(10061..10702)
                     /locus_tag="KPH29_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBG1468525.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVILQI
                     LMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKQNYSVGIILPWTLDDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFIYSYLMYKISGKNN"
     gene            complement(10683..10976)
                     /locus_tag="KPH29_002947"
     CDS             complement(10683..10976)
                     /locus_tag="KPH29_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG1468526.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            11370..11429
                     /locus_tag="KPH29_002948"
     CDS             11370..11429
                     /locus_tag="KPH29_002948"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468527.1"
                     /translation="MIEYIYYYNNKRIKKINLA"
     gene            complement(11945..12613)
                     /locus_tag="KPH29_002949"
     CDS             complement(11945..12613)
                     /locus_tag="KPH29_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBG1468528.1"
                     /translation="MPVLMAIGVPVHLAIGTNKFAASAGCISSAYRYAKSGKINNDLL
                     KKLVPFTIIGSVLGVRCVLSISEEILNVLVVVMILIVAIYTFISKNLGQEDNFEAVNK
                     KNLRLGMLMALIMGFYDGFFGPGTGTFLTFGFIKIYGYDFLHASANTKILNLTSNITS
                     LLLFMINGQVDYKIAIVFALVMIMGAYVGAKVAIKKGSKMIKPIFLVMALFMVVKLVY
                     QTLV"
     gene            complement(12558..12698)
                     /locus_tag="KPH29_002950"
     CDS             complement(12558..12698)
                     /locus_tag="KPH29_002950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468529.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIAGGGGTYKHACSYGNWSASTSCHW
                     YK"
     gene            complement(12757..13026)
                     /locus_tag="KPH29_002951"
     CDS             complement(12757..13026)
                     /locus_tag="KPH29_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1468530.1"
                     /translation="MNSISDVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLL
                     DRSMSISDICKKVGFNNSSYFSQIFKKFNSITPNEYRKNMLDNKY"
BASE COUNT         4831 a         1911 c         1292 g         5380 t
ORIGIN      
        1 tttaatatta aatattgacc ttcctcaacc gttatatatc ttcttcctga aaaagtatca
       61 ttagtaacta ttccctctgc attacctaag ctatcatttc taacttcata atatgcatca
      121 gaagaatttg acattataat atattctcca gcaggaattt ctaatccaat tttatacata
      181 ccatttccaa acttaccatt cttggccttc tgggctttag catactttgc taatgtcatt
      241 gttgaatcct caattttaat atattgacca ttctttaagg tgatatatct agtcccacta
      301 aaaatatcat ttgaaagtat actatcagca ttacctgtac tatcacttgt aacttcataa
      361 taagaagcat aacttcctga gtttgaagta attaagtatt cacctgctga tatatctttt
      421 ccaaccttat acattccatc ttttatagtc tttgtagtag gtttaacatt ttttacaacc
      481 ccagtattaa tatctccaat tccatttgtt atatttaaac actgttgtac tatatttgaa
      541 tctatatatc ctatagaagt tatcttctta gcatttttta aaatgctttt attgctacca
      601 tcattaacta aaactattgg accatgtttt gataaagaag atcctacaag tgcatttgtt
      661 aattcataag ctccagctat ataaaactct ctagcatcct tataaaactt atttattatt
      721 gctttattag tttcaaaacg agtactccct cctagtctag tagatttagt attgtttact
      781 aaagtggtac tcattgaagc tgttcctcct attacataag actttaaacc actagttgaa
      841 aaaggtatac tttgtccatt agtaagaatt attggtgctt tatctcttgc tgctacagat
      901 gcaatactta tagcatctgc ttctccttta tatgcattgg ttagcataac agtattgact
      961 ttttttattg aatttatttc ttttgcaaca ttataacttg tttttattct gtcatttccc
     1021 ttaattctta caactttcat tttcttagat tttaatgaat tttcgacaga tgtactaata
     1081 gaatatgttc ctccaattat ataaactttt gatacatttt ttaatcttgc agatgtctca
     1141 gttggtatag tatttttttc agttaatagt ataggggcat ttaaagctcc tgctagtcca
     1201 cttgcactta atccatctgc aatagagtta ctagtattaa ttaatatagc agttttatat
     1261 gcatttttat ctgctatttt gcctgctatt tcatacttat tttttccttg tattttttct
     1321 acagatgata ttgcatctac taattgaata tttgcaaata caaaagaaat acttaaagtc
     1381 aatgttaata ttcttttata gaatttcata ataaaattgt ccccctcata ataaattttt
     1441 aatatataaa tacatattct taaattagtt atgtacttaa caaacttatg taaaattgca
     1501 aattatatta agcatattac aaataaatat taatacaatt attcatttag ctataattgt
     1561 atttacattt atattaaaaa tcaactagat ttgtataaat acttataatt atatttttat
     1621 tcttaatatt ttttttatta tgttatttta atagatttat ttttcttttt taagcataga
     1681 gatgtatttt tccatatatt ctatttctat tatagtttcg cttatttaat tcacccaaaa
     1741 tttcacttaa tattagaatt ttaaatcttt tactaaaaca gacttttata gttctattaa
     1801 aattttttac taaactttat tttatactat gaattatcta ttttttttgc taaatttaag
     1861 caagttttcc acaaaaaata tatacattac tatattttta aaactctata aatatataat
     1921 tgatttaatt ggaaatttta gtgtattcaa aaaatgagaa atcagaggag attaatataa
     1981 aaatttatct tgaaaagtca attaaatata tcttatattt aataactaca aatgagatac
     2041 atataagaat attatatagg tatgataatt tatctgttaa attaagtgta attggtacta
     2101 tataaatatt ttattaaaaa taaatatata ataccaaatc tttaaacaac ataagaataa
     2161 aatagttata atctacttta ttcttatgtt atttagttat tataatgctt tataaagtaa
     2221 aatattattt cgtagttaag cttgataact aactcttctt attgatagac taacaatctt
     2281 gaataaaaat attcctgcaa ctagatttat taaaacagct ggtaatacta ctgctaagaa
     2341 taaagctgta aatgaagctc ctggaagacc aactatagct tgagctgcta ataaaaagat
     2401 agttccactt acaagagttc ctattggcat tattattgca actactacag tatcttgtgt
     2461 tttcttagct aattttttta taaaaggcat attataacta atattcatta ataaaaatac
     2521 tatatttgct gtaagagttt tatctattac attaggaatt tgacctcccg gaaaacttga
     2581 tgtaagtgct gtaaatattc ctgttactat acccgctatc aaacaagttt tataatcatc
     2641 tctatttaaa accaatatta caaataacat tgctagtggt acatctggct tcattggtaa
     2701 tcctatagct ggtgtgattt gatgtattaa taatcccatc gctaaaagta ttgcatttaa
     2761 agttaatttt tttgtatttg ttttcatttt ttgtacttcc tcctattttt ttaattttca
     2821 atttattttt ttgccaacct actatatcat catctttagc ttttgaaatt cataacatct
     2881 ttctcaccac cttctatctt ttaaattttc agtaaaataa aaaactccgt cctaaactaa
     2941 taggacggag attatccgcg ttgccaccta aattatataa aaattaatat aaaattcaat
     3001 tttcatacat ctcattatca ggtacttaca taccttattc gctataacgt gcgactgacg
     3061 ataaagtcta ctctcaaaac tatgatttca atttattcct ctaagggcca ttcatcaaat
     3121 tttttcttgc taagattcca ccatcctcag ctctctatac tgaacctaaa tttaactact
     3181 cttccttgtc ataggattta atttattgta ttgaattttg ttgttaaaat aatgatattc
     3241 ttttttctaa aaaatgtcaa taccttttta taaaaaatat attttgtaat ctatttttta
     3301 taaaaaaagt aaattatact atacatttct tttcatatac aaaattctat gaataattac
     3361 tttaatggtc taatagtctg ctctttggct ctaattcttc ttatattaat tttgtgaata
     3421 actgttgatg taattttata ttttgattca tgaggaatta aattcttcaa agttaattaa
     3481 gtacatttat caataaattt acttaattaa ccatcattta atactcattc ctgtatttat
     3541 atgcctcctt tgaaacagaa tattactcca ttttaattac atcatattat taaatagata
     3601 ccaatgatta gaaaaatgat attcttttaa ttatttttag taaaattttt tcttctttct
     3661 tttaataaat attagcacta ctaaaactat aaccaaaaat atgactatta tggacaatac
     3721 taattttgtt aggatattat gtcttcttgc atttcgtctt ttatatttat ttatagtttg
     3781 atatccatac tttttagttc ttatacttcg ttttttcaac tttactttac ttttattata
     3841 tttcctactt ttatgtctac ttttagattt aatagtttta aatttagtat tatcatatgt
     3901 ataattctct tttattgaag aaatgtatac actagactca ataatagctt ctgcttctac
     3961 ttgtaatgga tttataatac tgattataag tagaaaagat atagctatcc caatcaattt
     4021 tttcttctta ttcatatttt ccccctcata aaaattttta actagttcat tataatatat
     4081 atataactaa aaattacaaa ttttttcaag tttcttatat atcttattat ctttaatatt
     4141 atttttatta tcataacttt tacttttttt aaaatttttc aaagtagtat taatttatga
     4201 tacttggtgt ataaagtata agttaaatct actttcaatt aaatttacca tttataatta
     4261 ctttataata aaaataaatt agttaattta ttctctttag ttctgttata atgtttctac
     4321 aaaaaagaat cccattaatg ggattctttt ttgtgattta ttaacatatt taaagctcac
     4381 tagtatcgta ttataaactt aaatgctctt ctatgtattg attatataca tatccagaat
     4441 atcgtttttc atttttattg atattaactc ttgcccattt tccaacgcta taacatactt
     4501 caactttaaa accatcaggg attgtagtta taactttaga ttctaatgtt ctatcatttc
     4561 taagttctaa acttaatcca tttgttctta tatttcctat gtaattcttt tttctatttt
     4621 ttattgtatc attactattg tttttagcat tacttaatct ttctaaatat ttataaagta
     4681 accagccttt gtcataatta ctctcaactt ctgcccatat tccatctaca cattttaact
     4741 taacaatttt accttctggt atacttttta atttcaaact atttatgtct ggtttttctc
     4801 ttaaattcaa tttaatttta gttttaaaat tgatgctaga tttgccaaaa ttttttaaca
     4861 ttaattcaga tatcatctta agccctcctc ttgccaaata ttaagttatt aaaatatata
     4921 actctaaaaa tacttttaac aaaaagaatt attatctttt tggcaactgg ctgacatgac
     4981 ctttattaca taaagatttt agtccctata gttttgcgtc attagattta tctaatattg
     5041 cctatatctg aataataaca taatttgaac aattaaaaaa taatattacc tcaaatttta
     5101 tattatttaa atataaatta aatttttaat taataataat caaaaaatat ctttatattt
     5161 attcaattac tgttctttat agtcaatata aaagacagta atttatctat agtgttactt
     5221 atattttata tatatggcta tttttcaaca ttttacaata tatataacat ttattttttt
     5281 aacttttgtc agtaaaatgt cagcaagaca ctttatccac aatttttata taagttttcc
     5341 acaacttatc cccagtatta tccacataaa aaaggtagct cttatattat tagctacctt
     5401 ttatttatta atcttcatat ttccagcttt ttatccaact tcctatttct attatttttt
     5461 cttcagttat ttcttctcca tcatgtaatt ttgaaattaa ttctgataaa ggattgttat
     5521 gtaaacctct aaaaactctt tttccctcta ttttgagata ttctctctct tttattaata
     5581 ctgtatagtg tacatgcctc ccaattctct atgatattaa aaaacctttt ttggttaatc
     5641 tagctaaaag tataattgta gttgtatgct tccaaccata tgttttttct atttcatctg
     5701 caacatcttt tgatattaca gttttataac cagtattcca tatgcatttc ataattttta
     5761 attctgtatc tggtaatttt ttatttagca taatatattc cttctttcaa tttatgtcac
     5821 ttcttcaaat ttatctagct taaattataa taggagtttt ttagaacaaa aactatagtt
     5881 aatttttacc aggtactcta cgtgtttagt ctgtttttta agtgtaaaat aatgttcata
     5941 tctataaatt tatttaactt tcaaacaatc ctgttgaata ataactaatt ctattgtatt
     6001 attttagttg tagaataaaa ctaaatcggc aaaactagag aaatttagtg acgcaaagct
     6061 atagggacta agacttatat aaatatctta tgagttatgt cagccagttg ccaaagagat
     6121 agtgttcttt ttgtttttat gaaagttttt tagggggata ataatttata tgtttggaaa
     6181 taaaatggat aaatgtacac atgtattgac tgcttatatt agcggttcgt atgattattg
     6241 taatttttta gatacacaat tagatgattt tatattagag tacggagaaa gtgtagtaga
     6301 gtcttgtttg caccaagtga tggtattggt aagtaagtat aattagatag acattgattt
     6361 ctagagaacc ctttttagtt ttatctacat ttatattaat atatagcaat gtaaaacgct
     6421 taaatatatt tttaatatta agaataacta aaatgttgac taaaaaatat aattaagtag
     6481 acatttctat taatctatga tacaatagta tttgtggtaa tacacaatta aatcaagtat
     6541 atcttgatat aggttaatag gagtattttt aatgaataat ggaatagtga aatggtttaa
     6601 taatgaaaaa ggattcggtt ttatatcaat ggaaggtaga gatgatgtgt ttgctcattt
     6661 ctcagctata caaacttcag gatttaaatc attagaagaa ggtcaacaag taagctttga
     6721 tatagttaaa ggtgctagag gtcctcaagc agaaaatata actatattat aatatataga
     6781 tttttcggga aagactcttt ttagagtctt tttttattgt tagtatatgt aagataatca
     6841 ttggtgaagt aaataaggta tattgagtaa tgttcttgat agtttatatg ctaatgaatt
     6901 tcaaaaaaat aagcactcct ataaaaagag tgctcttaat gtaaatataa gccttcattg
     6961 gtccagccta ataccccgta ttctccaagt tctaactctt ttttatatac attaccactt
     7021 tcagccatat ctaacacaat catttcttct ttagaatcaa tttgtaaaat atgaggactg
     7081 tgtgttgtga caattatttg tgtttttagg agaattgctt ttaatgtaat aaaaagttta
     7141 catacaacat agttaatata aaacaaaatt atttttgaga atgagctaaa taaaatctta
     7201 actttacata ccacatagtt gatataaaac cccaaaataa acttagcatc tccaatgttt
     7261 acacatatac aatgctctta aaattgcagt gaacatgcaa tagtgcaatt gataatactt
     7321 atcatatatt ctcaatacct tatatttcaa ctgctaaacc tcaatttgtc acaaatatcg
     7381 ctcactgcaa aatttctata tttttattat atcatagcat ataattaata ttttcaatta
     7441 atttttacat ttaattatgt ttaaattcta taataatgca actttaatgt ttctttctgg
     7501 aactaatata aaaactattt cagaaagatt aggtcatgca gatataaaaa taactatgaa
     7561 tagctactct cagtttttag aagagatgga cagagaagca tttgaaaatt tgagcaaaat
     7621 actatttatt gtattttaaa gaagatgact tattcaatat ttgatgctag aatataatta
     7681 ataaaaattt aattataggt gattgtatta tgaacattat taaaagcata actttactct
     7741 taacattatt aattgtgata ttaatgacag gatgttcccc aagagaaagt gctagtgaag
     7801 ttgcaaaaaa atatattgat aagatgaact ctggtgacta tgaaacagca tatagtttat
     7861 taagtaaaga tagtaaaaaa aaaattagta tagaaaatct tgaagaattt caaaatattt
     7921 tagttagtac aaaaagaata attagttata aaataggtaa agaaaaaatc ataaaaaaat
     7981 ataaacatga tggaaaagaa tataaaaatg ttgttaaatt agaagaaact tttaatataa
     8041 aaaaccttct ttataaaaaa gatgattctc taaaagtaaa tagatatttt gtagttgaaa
     8101 ataacaaata caagctatta ttgtatgaaa gtttcaaaaa agaatttggt attaatagta
     8161 tggaccttgc tcaacttaag cttaatgatt ttggactaaa atactttaaa gaagttgacc
     8221 ttatactaaa aaaagctatt gctattaatc ctactgattc aaagctttat tatgcccaag
     8281 catgtaatta tatgaatttt gatggatttc ctggttattc agatacaaca tataatgctt
     8341 tagattcaat taataattct ttaaagtatt tagataaaaa tgattctgac tacaaaaaaa
     8401 atgcaagtaa tatatataac ttaaaaggag ttattcttat gtttcacaaa agatttgatg
     8461 aagcacaaca atatttaaat aaagccttat actttaacaa agataatgaa gacccaaaat
     8521 ataatctaaa tacaataaaa aaacatctaa actagatatt taatttttga atttataaaa
     8581 tattttttta tgtataattc agcactgaat cataatctct actaaactaa ttacaagttt
     8641 aaattataaa gacttatttc tatataaata ttactctata acaataagtt tcttatttaa
     8701 attttaaaat attatctaaa aatcataaaa aagaaggtat aaagagctac cctcaagctc
     8761 aatatacctt ataaatatct aaacttcatt ttttactaat ttttctatat ctttgataat
     8821 atgttcttta gatttttttc cagctatggc atcaaaaatt gcaacagcct ttttctctcc
     8881 aaacttttct gctacatcat taaagtctaa aaatctaaca ctttcttttg gtttaccaaa
     8941 catataacac ctctcataca tttttatata tttattataa cttctataga cttatttttc
     9001 accaataaac gttagacaaa ttagtccctt tattttttta aactagcttt accttcaaaa
     9061 ttcttaacaa aaacagttta ttctactcta taaataaaaa tagacaattt aatttgtagg
     9121 aaactgataa tagaatacag taaataaaac tttgtttaat ctatctgaaa aagttatcta
     9181 ttaaaactgc tatgaattta taaagaatat tctagttcat aaaaatacaa atatgtaaat
     9241 ataatcacct taatattcaa tttatttaaa ataaaaaatg tataaaagat ttaataatga
     9301 caatatacta ttctcaatac aatctatgat gtaaataatt taaaactact atattattta
     9361 aaaatttata tttatatatg caattctaaa aacttaataa gaagtgaact tagaaaataa
     9421 agcttaattt caaagactta aatagaatat tattattagt ctaaataaac tacgtgtatc
     9481 aaaattatat attaaattct tctaaaattt ttattgcaaa tcttgcgttt ggatatgtaa
     9541 tagacccgcc accaattaca cctattttta tagcttctat aatttcatct ttacttgctc
     9601 cagaattaat acaaccctca atatgataac aaatacactc atcacatcgg cttaaaattg
     9661 aaatagctag tcccattaat tccttatttt ttttacttat agccccttct gaataagtct
     9721 tatcatcaag acttccaaat actctaaaaa actcatctgt ttcacttaat tttttattgt
     9781 aaaatcccct atattctttc atttctctag tattcatctt ctacctccta tgtttttctt
     9841 ataagagtat cacactgtac ttatattttc aatattaaat acaaattttc attttatgtt
     9901 cattagccta atttatatta ttttttgaaa tctcaataag ttatcatata agtaagattt
     9961 tttcagaact aaaatagaaa tcttagtaat ttctttatta tctatctcag ttctgaaata
    10021 tattattctt atccaaacat tattatagtg attaattata ttagttattt ttacctgaaa
    10081 ttttatacat caaataagaa tatataaatg gtagtattac aattacaaag attacaaaga
    10141 tattataaaa aggaatatta ataaatgcat taataattaa taatattcca ccaataagcc
    10201 aaatccatcc agctaaatgg tgtgtcttat tccaattatc ttcatcatca agagtccatg
    10261 gcaaaattat tcctactgag taattttgtt tacatttagg aagatagttt ccaaataaaa
    10321 taactattat tcctaaaatt aatcctgtat aaaaattaga ctccaaacta actcctgttg
    10381 aatataaaac catcagtatt tgtagaatta ctgaaagtat agcaactccc cattttgata
    10441 aattttgtat aacatttgaa aaattagctt ttttaggctc attctcaagt ctaaaccatg
    10501 tatacaaatg gatacatagc attaacaatg gtacaccaaa agctgctata acctttggag
    10561 cataattatc tggatttcca taattatcaa aatgaattgc aacttcattt ggtaatctat
    10621 tataaaaata tattgataca ataaatggta taaaacatat taaagtagat aaaataaatt
    10681 gtttatttat cgcttttttc attatctttt tctcccttct ccataaattg tgaaaaccaa
    10741 agcataacct cttcgaaaac tgaaatattt atttcgtaga aaataaagtt cttatacttt
    10801 gtttcaaata tgagtcctgc cttttttagt tgagatagat gataagaaat tgtagcattc
    10861 gtcatattaa atttttcact aatttctcct gctgacattt tattctccct caacataacc
    10921 aatatttctc ttcttatagg gtctgaaaga gctttgaatg tttctggaaa ccccaacacc
    10981 atcacctcgc tatttagaat ttcatctaaa tagaatataa cataacatcc attttatttc
    11041 aataactatt tagaaaaatt tctaaatagt tatttccaaa tatttattga aaattttcat
    11101 attaagattc tcaatttact taagtcgctt ttatataatc ttattttatc taattttttt
    11161 aatttaacaa aagtgtattg caccacaatt gttaaagcaa aaacctaatg actgatatac
    11221 ttatatatat tacatatact tttaatttca aattaaaagt agctaaatcc aatttaaaaa
    11281 gtagttaagt cctatttaaa tagaaaagaa tgaaataaga aatatactgt ataaaaatgc
    11341 ataaaaacct ctactaatta gatacaacta taatagaata tatttattat tataataaca
    11401 aaagaattaa aaaaattaat ttagcataac cttgttaaat atagatttga atatgaattt
    11461 aaagttatgt aaaattaaaa atacaggaat gaaaattcca ttcctgtata aaatctaact
    11521 tttgagtctc aatacaatct tattttgaga caatcttttt tgttttatat tttcttatat
    11581 ttaaaatata acgcatatct tatgcaacat attcatattt ttctttatat tctttcatcg
    11641 catcattcca agcgtcttgt actgttacac ctcttttatt agcaattttt tgtgccagtt
    11701 caattatctt catagctctt cttgatatgt tcataaacat tcctcctaca aattaccata
    11761 tatttcatat atataaatca aggttatatt tttatgatat ataatccttc tattgtaata
    11821 ttacaattta tatgtgtatt atatatgaac tatattagaa gataattaga atttttataa
    11881 agctatatga ttagattaaa tataaaagct ttacaattac ttataaattt aaaaagtcta
    11941 taaactaaac taatgtttga tataccaatt ttacgaccat aaataatgcc attactaaaa
    12001 atattggttt tatcatcttt gaacctttct ttatagcaac tttagcacct acataagctc
    12061 ccattatcat cactaatgca aacactatag caattttata atctacttga ccatttatca
    12121 tgaataataa caatgaagtt atattacttg ttaagtttaa tatttttgta tttgcagaag
    12181 catgtaaaaa atcatatcca tatattttta taaatccaaa agtcaaaaaa gttcctgtac
    12241 ctggaccaaa aaaaccatca taaaaaccca ttataagagc cataagcatc cctagtctta
    12301 aatttttctt attaacagct tcaaaattat cttcttgacc taaattttta gaaataaagg
    12361 tgtaaatagc cactattaaa atcataacca caaccaaaac atttaatatc tcttcactta
    12421 ttgataatac acatctcaca cctagaacac taccaataat agtaaatggt actaattttt
    12481 ttaaaagatc gttattaatt tttccagact ttgcatatct ataggcacta cttatacatc
    12541 ctgctgatgc tgcaaattta tttgtaccaa tggcaagatg tactggcact ccaattgcca
    12601 taagaacagg catgcttata agtccctcct cctcctgcta tagaatctac aaaagcagct
    12661 aaaaaccccc ctatacataa aaaaataact gaaaacataa aattcctcca tcattctctt
    12721 tcaatttttc ttacaatata tattatataa tcttatttaa tacttattat ccagcatatt
    12781 ctttctatat tcatttggag ttatactatt aaacttctta aatatttgtg aaaaataact
    12841 actgttatta aaacctactt tcttacaaat atcactaata ctcatgcttc tatctaacag
    12901 atatttttta gattcttcta tcctaagaag atttatatac tcagttattg aaactttatt
    12961 tctttcatta aaaatagaac tcaaataatt tggacttata aaaaaaacat ctgatatact
    13021 attcaaattt atattttctt tataatattt ttttatatat tcgtttatat tttcaattag
    13081 atttccttta tcttttctcc aaaatgctct attcttatca atacactctt tggaaaattc
    13141 aaataataca ttccataaat cttcatcact gtctacaagt gatatttttt tattatattt
    13201 atttcttaaa taagatataa gttttaaatc ctctaaatct ctaagcactg ttctagacaa
    13261 tacaaatatc atttcctgaa tagtatctct aacatcatta tatcttaatt tgtttgtatc
    13321 tattagatta taaacacctt ctaaattcat atttttaatt tctttttcaa tataaagttc
    13381 taattcaaag ggatattctt ttgaagaatt attg
//
LOCUS       DAELXX010000106        13285 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.106, whole genome shotgun sequence.
ACCESSION   DAELXX010000106 DAELXX010000000
VERSION     DAELXX010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13285
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.106"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            36..494
                     /locus_tag="KPH29_002952"
     CDS             36..494
                     /locus_tag="KPH29_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1468531.1"
                     /translation="MEIIDYIEENLTENLNLDKIAYIANYSKYHLHRMFTNVVGCTIH
                     QYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQVYRNKDEF
                     YPIQQRFVLNLNHQIQSEKCISYANMNYKQRRDEINSYLNFNINCEVKVA"
     gene            491..1075
                     /gene="clpP"
                     /locus_tag="KPH29_002953"
     CDS             491..1075
                     /gene="clpP"
                     /locus_tag="KPH29_002953"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBG1468532.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            1397..2473
                     /locus_tag="KPH29_002954"
     CDS             1397..2473
                     /locus_tag="KPH29_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG1468533.1"
                     /translation="MIAISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTNGGFKSNDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            2540..4579
                     /gene="bclA3"
                     /locus_tag="KPH29_002955"
     CDS             2540..4579
                     /gene="bclA3"
                     /locus_tag="KPH29_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HBG1468534.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGGTGPTGPTGPTGPTGNTGNTGATGLR
                     GPTGATGGTGPTGATGAIGFGVTGPTGPTGATGATGADGVTGPTGPTGATGADGITGP
                     TGATGATGFGVTGPTGPTGATGVGVTGATGLIGPTGATGTPGATGPTGAIGATGIGIT
                     GPTGATGATGADGATGVTGPTGPTGATGADGVTGPTGATGATGIGITGPTGATGATGI
                     GITGATGLIGPTGATGATGATGPTGVTGATGAAGLIGPTGATGVTGADGATGATGATG
                     ATGPTGADGLVGPTGATGATGADGLVGPTGPTGATGVGITGATGATGATGPTGADGLV
                     GPTGATGATGADGVAGPTGATGATGNTGADGATGPTGATGPTGADGLVGPTGATGATG
                     LAGATGATGPIGATGPTGADGATGATGATGPTGADGLVGPTGATGATGATGPTGPTGA
                     SAIIPFASGIPLSLTTIAGGLVGTPGFVGFGSSAPGLSIVGGVIDLTNAAGTLTNFAF
                     SMPRDGTITSISAYFSTTAALSLVGSTITITATLYQSTAPNNSFTAVPGATVTLAPPL
                     TGILSVGSISSGIVTGLNIAATAETRFLLVFTATASGLSLVNTVAGYASAGIAIN"
     gene            4748..5509
                     /locus_tag="KPH29_002956"
     CDS             4748..5509
                     /locus_tag="KPH29_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HBG1468535.1"
                     /translation="MKNKIETSMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLENGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKMGANSYAIPGSYNFDSEKLVSDWSQRHVAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            5818..6096
                     /locus_tag="KPH29_002957"
     CDS             5818..6096
                     /locus_tag="KPH29_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468536.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGEYIYIPRKSNNKKEWGSSTS
                     TREDLSIRNMQIYEDYQVGYDLKYLSEKYYLSLKSIQRIVLQEKRKDS"
     gene            6389..6964
                     /locus_tag="KPH29_002958"
     CDS             6389..6964
                     /locus_tag="KPH29_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HBG1468537.1"
                     /translation="MKKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPKSLNPETLEQFHTNDYNLAKQYAMNTELLHRNG
                     AEVGIPEYKIVGQEININDLTQVHIISKYPCQLKVSTLLREKLGLSRKVFDEMLDCGI
                     IRSNSGVDLKKGKLNYEIMLDIGKEKQKSSN"
     gene            7402..8112
                     /locus_tag="KPH29_002959"
     CDS             7402..8112
                     /locus_tag="KPH29_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1468538.1"
                     /translation="MISIGICDDELHYRIKIKDILSEILSSYPINYNIYEFSSGEELL
                     NNYPKDLDILIMDIQMKTINGMDTARKIREFDHKLEIIFVTSFVEFMQEGYEVKAYRY
                     ILKPINKEKISKSVLPCINEMMKKRNNYLTINVKNYVDRIKIDSITYIETDRPNILIY
                     THDDMYITKMSISKIEKILNEYGFFRCHNSYIVNLKLVESMSGSTVIVDGKSIPISKY
                     RVKGLKLAITNILGDIVC"
     gene            8106..9422
                     /locus_tag="KPH29_002960"
     CDS             8106..9422
                     /locus_tag="KPH29_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG1468539.1"
                     /translation="MLTNLSIGFWNIINLVSGTIDWIIFFLVIHLVGKRKISNIRYIF
                     TTIIIFIFMAYINVVKVFPNIKILLCMVIGISFYITSYEDKIYKSIIVSLLFWLGLMI
                     AEGSAVGLVVFINKLNNINITQHENLFRIQAIIMSKVFLFIGFILFKYFKLSLDFKPK
                     DMILIGIPILSNIVSLLLIFEYNLRVNTITTENLFIFIFAILLILSSSIILLIVIGKI
                     VQDDKLNLEYELINERIKTNYRNYENINEVHDRLKYVYHDLKNHMICIKNYDTKEEII
                     SYIDNLEFQISEFEHFKNTGNKTLDLILAEKISICKKYDIQIEDNINISKLNFIENND
                     ICSIFANSLDNAIEACMDINNELEKRIEVKATYINKFAIIKFINTKVNDIKLIDKRIQ
                     TSKDDDKIHGIGLASIKYIVNKYGGETIVNYSDNEFILKIMIPIKS"
     gene            9434..9574
                     /locus_tag="KPH29_002961"
     CDS             9434..9574
                     /locus_tag="KPH29_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBG1468540.1"
                     /translation="MKKIALNLLKNISALSFGIAVLSANSASSWVAHQAKEPQALQKL
                     KK"
     gene            9633..10214
                     /locus_tag="KPH29_002962"
     CDS             9633..10214
                     /locus_tag="KPH29_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBG1468541.1"
                     /translation="MFKRLSYKFANILVNNEIVESEDFEIYRYGFETLIYFIVNISVA
                     LFIGIIFDRFIHTVIFLSCYCTLRQFTGGYHARNYKECTLTFAVIYLITIFSANNIDI
                     NKYKYLLVLLMIISILTIYKLAPLEHRNKPLSESEKKHYRKTVQKILFVIICLIILCK
                     ILNIFQQYVIYAIISIYWIAILIYIGMKVNNDQ"
     gene            10291..12069
                     /locus_tag="KPH29_002963"
     CDS             10291..12069
                     /locus_tag="KPH29_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468542.1"
                     /translation="MNLLNIVESIVSCIKGLARRGINMSSIKVISDFITKIIRKNILF
                     AITVIIINYHLIQRWTHISNRGISFNDYLSLSVYTCMTISILTMLNVIFYMRKSYDTV
                     KILCRNNFDYFLGISVAVTRNNFIFCCIPIIYAIAYSSLIRTLSIVTIKSTIFILVFE
                     WFLPLTILSIVISFISVIINNLILKITLSSVFLYLTSTKMLDRVIDIKEPFFKALFKS
                     LNIFEDQSYAYYSDFVGKVNNSAYVIDKIIPTLFAITLIIVAYLILNDIRKKQIKSLI
                     VIVVYSILFIGLNYISIDMSTYRDEERSYGEYLKKSNISDIKIKSHDMKIDFKSKSNI
                     KDRIVLLNTSNEELTNINMILDEIFDIKYIKVNSENIKFNKKKDLIEVNLKESLEPND
                     EVTLDIEYEGYINVLTSWNQNKYIATKDDIMLPPGSLAWYPKVNQSSCMAYKINLSSK
                     NNVYSNLPLLNKTSNFLKKDYIFEGKSNDIAIYAGEFKEEVKDDIKIIYPSDSNVNMD
                     IEEKIKFLIETRNEILGDSKASVDAKKYVDEVDNVMESIKRKKINMILVADLPSNEFL
                     YEDSKGEKISITIYHWLSGNILIIDK"
     gene            12084..12956
                     /locus_tag="KPH29_002964"
     CDS             12084..12956
                     /locus_tag="KPH29_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG1468543.1"
                     /translation="MINKLEIRNLNFNYKNKKALDNINLTLTDGVVALLGPNGAGKST
                     LMRLLVTLYETSIGEIELNNIKYSKNNEKIKANVGYIPQDFDMYNNINGQEYLEFVAK
                     MRGVSKNDLKKHIQKVVSKVNLDKFINNKIGTYSGGVKRRLGIAQALIGDSKLIVMDE
                     PTVGLDPEQRNEFRRLLPEISKNSIVLISTHIVEDIQFNCDKLIILNQGKILYDGTIN
                     KFIDMVEVYSVVVSNDEFEYLERNIDIIDYRRVKDGVQVKYIGKNTEKLKDSLRIDTT
                     LQDAYINFLHKELK"
     gene            12957..>13285
                     /locus_tag="KPH29_002965"
     CDS             12957..>13285
                     /locus_tag="KPH29_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468544.1"
                     /translation="MCILELIKFELKKIELKHVLIFVIISILCIFITGILDYNELIFY
                     NDVPIMDTYSIAGWLGIIKISDVFIPIMSAITIIYVFSKDYLGNINEILTLYNKKKYN
                     QFIVFRW"
BASE COUNT         5329 a         1485 c         2352 g         4119 t
ORIGIN      
        1 agaggataga gttgaaaaca aatacagatg ttattatgga gataattgat tatatagaag
       61 aaaatcttac agaaaatcta aatttagata aaatagctta tattgctaat tattcaaaat
      121 accatttaca cagaatgttt acaaatgttg taggatgtac tatccatcaa tacatacaga
      181 ggaggagact tactgaagca gctagacaac ttatatttac agacaaatat attatagata
      241 tagctattac agtaggatat tctacacatc aatcttttac actagcattt agaaatttat
      301 ataaagaatc tccacaagta tatagaaata aagatgaatt ttatcctatt caacagagat
      361 ttgttttaaa tctaaatcat caaattcaaa gcgaaaaatg tatttcttat gcaaatatga
      421 attataaaca aagaagagat gaaatcaatt cttatcttaa ctttaatatt aattgtgagg
      481 tgaaagtagc atgagtttag tgccacatgt aattgaacaa acaggtcaag gggaaaggtc
      541 ttatgatata tattcaaaat tattaaaaga tagaattata tttataggag aagaaattaa
      601 tgatgctatt gcaagtttag tagtagctca attactgttt ttagaatcag aagacccaga
      661 ttcagatata attatatata taaatagccc tggtggctct gctacatcag gatttgctat
      721 atatgatact atgaattatg tcagatgtga tgtatcgact atctgtattg gtatggctgc
      781 aagtatgagt gcttttttac ttgcaggagg aacacatgga aaaagatgtg ctttacccaa
      841 ttcagagatt atgattcatc agccaatggg aggagctaaa ggtcaagcaa cagatgtaaa
      901 aatagctgta gataatatat tgaaaataaa agaacgatta gataaaattc tttctgaaaa
      961 tacaggtaaa tcaatagaag atattagaag agatacagat agagataatt ttatgactgc
     1021 actagaggct aaagagtacg ggctgattga ttatataatg aataggaatg aataggaatg
     1081 aataaagtat caaagtatca tattaaaatg tctgttttac aaatatatat ataatattaa
     1141 ataagagtgt ctcaaaatga actttttagt ttatgggaca ctcttttttg tatgaaatta
     1201 aatatggttt tatcatttca acaattatta ccaaatttca agtttaataa attctactat
     1261 aaaaaataca taaaagataa ggttctttag taactttctg tcgtcaaaag tttttataaa
     1321 tttaataaca aaacaagaga aaataccata tattacttat gtctgcaata tatagaagac
     1381 aaaggagttg tacattatga ttgcaataag tttgtgcatg attgttaaga atgaggaaga
     1441 agttataggc agatgcttag aatgtgtaaa ggatatagta gacgaaatta taattgtaga
     1501 taccggttct acagattcca caaaagaaat agtctcaaaa tatacagaca tggtgtacga
     1561 tttcgaatgg atagatgatt tctctgctgc aagaaatttt tcattttcta aagcttctaa
     1621 agactatact atgtggcttg atgcagatga cataatttta gaagctgata gagaaaaatt
     1681 attaaagatt aaagaaagct tggatacttc cattgatatt gttatggcaa aatacaatgt
     1741 atcctttgat gaaaatggta acccaactct ctcatattat agagaacgat tgtttaaaag
     1801 aagttctaat cataaatggg ttgaccctat tcatgaagta gtacctcttt ttggagaggt
     1861 attttattct gacattgcaa tatctcataa aaagcttcat agacaggacc ctctaagaaa
     1921 tcttagaatt tttgaaaaaa tgatttcaga aggaaaaaca ttggagccaa gacaccaatt
     1981 ctattactct agagagttgt actataatgc taggtataaa gaagcaatag aaggttttac
     2041 taagttttta gactcatcaa gaggatggat tgaggattgt atcagtgcct gcagagattt
     2101 agccacatgt tattacttaa ttaatgatga aaaaagtgcc ttatattcat tgtttagaag
     2161 tttcgaattt gatgagccaa gagcagaaat ttgttgtgac ataggaaagc atatgtttga
     2221 tagacaaaaa tataaagaag ccattttttg gtataaggta gcattaacaa gagataagaa
     2281 tgatactaat ggaggtttta agtcaaatga ttgttatgga tatataccat ctatacaatt
     2341 aagtgtctgt tatgacaggt taggagaatc agataaagct atttattatc atgaaaaaac
     2401 aaaagaaatc aaacctaacg atagtgctgt tttgcacaat gaaaattatt ttagtaaatt
     2461 taaaaatagt tgataggtca caatcatact aaatttgaca tattatatat agagcttaaa
     2521 agctcaaata tatcaggagg tgcttttaat aatgagtaga aataaatatt ttggaccatt
     2581 tgatgataat gattacaaca atggctatga taaatatgat gattgtaata atggtcgtga
     2641 tgattataat agctgtgatt gccatcattg ctgtccacca tcatgtgtag gtccaacagg
     2701 cccaatgggt ccaagaggta gaaccggccc aacaggacca acgggtccaa caggtccagg
     2761 agtaggggga acaggcccaa caggaccaac cggtccgact ggcccaacag gaaatacagg
     2821 gaatacagga gcaacaggat taagaggtcc aacaggagca acagggggaa caggcccaac
     2881 aggagcgaca ggagctatag ggtttggagt aacaggccca acaggcccaa caggagcgac
     2941 aggagcaaca ggagcagatg gagtaacagg tccaacaggt ccaacgggag caacaggagc
     3001 agatggaata acaggtccaa caggagcaac aggggcaaca ggatttggag taacaggtcc
     3061 aacaggccca acaggagcaa caggagtagg agtaacagga gcaacaggat taataggtcc
     3121 aacaggagcg acaggaacac ctggagcaac aggtccaaca ggggcaatag gagcaacagg
     3181 aataggaata acaggtccaa caggagcaac aggagcaaca ggggcagatg gagcaacagg
     3241 agtaacaggc ccaacaggcc caacaggggc aacaggagca gatggagtaa caggcccaac
     3301 aggagcaaca ggagcaacag gaataggaat aacaggccca acaggggcaa caggagcaac
     3361 aggaatagga ataacaggag caacagggtt aataggtcca accggagcaa ccggagcaac
     3421 cggagcaaca ggcccaacag gagtaacagg ggcaacagga gcagcaggac taataggacc
     3481 aaccggggca acaggagtaa ccggagcaga tggagcaaca ggagcgacag gggcaaccgg
     3541 agcaacaggt ccaacaggag cagatggatt agtaggtcca acaggagcaa caggggcaac
     3601 aggagcagat ggattagtag gcccaacagg tccaacaggg gcaaccggag taggaataac
     3661 tggagcaacc ggagcaacag gagcgacagg tccaacagga gcagatggat tagtaggtcc
     3721 aaccggagcg acgggagcaa caggagcaga tggagtagca ggtccaaccg gagcaacagg
     3781 ggcaacagga aatacaggag cagatggagc aacaggtcca acaggggcaa caggtccaac
     3841 aggagcagac ggattagtag gtccaacagg agcaaccgga gcaacaggat tagcaggagc
     3901 aaccggagca acaggcccaa taggagcaac aggtccaaca ggagcagatg gagcaacagg
     3961 ggcaaccgga gcaacaggtc caacaggggc agatggatta gtaggtccaa ccggagcaac
     4021 gggagcaaca ggggcaacag gtccaacagg cccaacaggt gctagtgcaa taataccttt
     4081 tgcatcaggt ataccactat cacttacaac tatagctgga ggattagtag gtacaccagg
     4141 ttttgttggc tttggtagtt cagctccagg attaagtata gttggtggag taatagacct
     4201 tacaaacgca gcagggacat tgactaactt tgcattttca atgccaagag atggaacaat
     4261 aacatctatt tcagcatact tcagtacaac agcagcactt tcacttgttg gttcaacaat
     4321 tacaattaca gcaacacttt accaatctac tgcaccaaat aactcattta cagctgtacc
     4381 aggagcgaca gttacactag ctccaccact tacaggtata ttatcagttg gttcaatttc
     4441 tagtggaatt gtaacaggat taaatatagc agcaacagca gaaactcgat tcttactagt
     4501 atttactgca acagcttcag gtctttcatt agttaatact gtagcaggat atgcaagtgc
     4561 aggaattgca ataaattaga ttagataaga aaaggcttct aatatcgtat tgatattagg
     4621 agctttttta agaatgattg atgaacatag ttttaatatt aattagtgcg catattataa
     4681 aatactatta tataatataa aataataaca ttaaattgga aatatagaat tatctagggg
     4741 tgatactatg aaaaataaga ttgaaacatc aatgaacaac tttgtaaaaa attatcataa
     4801 atcaaataat ataactacaa agtggcaaga acttttgttt gcttatgcat ctgctgaaga
     4861 tgatatgttt cagaagctaa aaagtgttgt aggaacatct catgctatgc caaaagattt
     4921 tctggaaaat ggaaaaacta taattacata ttttatacct tttgatgaaa gtgtaaataa
     4981 tagtaatatt ggagggtttg aatgttctga aatatgggca agagcgtaca tagaaactaa
     5041 tatgttgatt tcaaatttaa atgatttctt atgtgaagaa ctagagaaga tgggggctaa
     5101 ttcatatgct attcctgggt cgtataattt tgacagtgaa aaattggtaa gtgactggtc
     5161 acaaagacat gtagcattca tagcaggtct tggaacattt ggtttaaata atatgcttat
     5221 tacagaaaaa gggtgttgtg gaagaatagg aagcataatc acaagtttag aattagagcc
     5281 aactaaaaga ccagaaaatg aatactgctt gtataaacat aataatagtt gcaaaaaatg
     5341 tgtcaacaaa tgcgtgaaag aagctttaaa agaagatgga tttaatagga ataagtgtta
     5401 tgaaatgtgt atgataaatg aaaaaaaata cacttatata ggtggttgtg atatttgtgg
     5461 aaagtgtata gttggggttc cttgttcggt aataaatccc tcaagataaa ggacttatgt
     5521 tgattaataa ctttaaatat atctaataag aagaaaaaag ttagtctcta aaatagatga
     5581 attatgaaaa taaataaaga taatataaag gttagatgaa gaagttatgt taattgacaa
     5641 ctatttatac tgctgataga cttatatgta tcgttgcaat atataaggag gggtgagatt
     5701 tatgtttgat ttaatcttta tttttgcata gcaagggcta ttgcaaatat aaaatttaat
     5761 atataaatag ccctttgcta ttcctaataa aaaattatta tttaatagga gtgcagaatg
     5821 agctataaaa aagcaataca tatattacca gaagaattac ttgaattgat acaggaatat
     5881 gtagatggag aatatatcta tattcctaga aagtctaata ataaaaaaga atggggaagt
     5941 agtacgtcta ctcgtgaaga tttatcaatt agaaatatgc aaatttatga agattatcaa
     6001 gttggatatg atttgaaata tttatcagaa aaatattact tgtctttgaa aagtattcaa
     6061 agaattgtat tacaagaaaa aagaaaagat agttaagaat gagccagtat agttttataa
     6121 ttatactggc tcattttctt ttgataaaag tgtttgaggt atagtttctt ataagtggat
     6181 gctataattt aaataatttc aataacttgt acagcagata acgaatagag ttgtaaagta
     6241 tctaataaga agtagtagct ttttatatgt ttgaaattaa aatttattaa aattaggagg
     6301 tcttgttttg aaaaaaatac ttgattatat tccacctttt cataactttc tattaattta
     6361 gtaatcttat agaaaggaag ttgcatttat gaaaaaaatt acttgggagg tggaatatat
     6421 ctcccagtta cctgtaatta ggtattgtaa gaaatgtgga aaaaagactg aatatatttg
     6481 ttcaggttta tttagggtta atgctcaaca taaatatttg gatatatggt taatatacaa
     6541 gtgttcaaat tgtgattcaa catggaactc aactatatat tctcgtgtca atccaaaaag
     6601 cttaaatcca gaaacactag aacaattcca tacaaacgat tataatcttg ctaagcaata
     6661 tgctatgaat acggaacttt tacatagaaa tggagcagaa gttggtattc cagagtataa
     6721 aatagtagga caagaaatta atattaatga ccttacacaa gttcatatta ttagtaaata
     6781 tccatgtcag ttaaaggtat caacattatt acgtgagaaa ctaggtttat ctaggaaggt
     6841 atttgacgaa atgttggatt gtgggattat tagaagtaac tcaggagttg atttaaagaa
     6901 aggtaaatta aattatgaaa ttatgttaga tataggcaag gagaagcaaa agtcttcaaa
     6961 ttgattagaa taactaactt aatatatttt caggaacatc atttaaaaaa atagtggctt
     7021 taaaaaattt aaagccacta ttttttaata tgttttatat gaatatttaa aatatagtgt
     7081 gtattcttca aaattatagc gataattaaa atattactat aaaaaggaag gtgcatactt
     7141 tttgtttaac aaaaactaga tactaaaaac ttctcgaaga atatgttatt aaaatcgcta
     7201 aatatattca atatatatta aaaaattgta ttataatgaa aattaaaata gattgtaaat
     7261 ccaaaaagta atacttaatg tccaaaaagc taaaattaaa atctaaatag ctatttgcta
     7321 cagaagtatg ttaaaatatg taaaatgaat agaaatttta tatatttatt aaaaatatat
     7381 aagaaaggat gtttaagaat tgtgattagt ataggaatat gtgatgatga actacattat
     7441 cgaataaaaa taaaagatat attaagtgaa atattaagtt catatcctat aaattataac
     7501 atatatgaat tttcatcagg agaagagtta ctaaataact atcctaaaga tttagacata
     7561 ttaattatgg acatacaaat gaaaacaata aatggtatgg atactgctag gaagataaga
     7621 gagtttgatc ataagttaga aataatattt gttacttcat ttgtagagtt tatgcaagaa
     7681 ggttatgagg taaaagcata cagatatata ctaaagccaa taaataaaga aaaaatttct
     7741 aaaagtgtat taccttgtat aaatgagatg atgaaaaaga gaaataatta cttaactata
     7801 aatgtaaaaa attatgtaga ccgaattaaa atagattcta ttacttatat agaaacagat
     7861 agaccgaata tattgattta tacacatgat gatatgtata ttactaaaat gagtatatct
     7921 aaaatagaaa agatattaaa tgaatatgga ttttttagat gtcataatag ctatatagtt
     7981 aatttaaaat tagtagaatc aatgagtgga agtactgtta tagtagatgg caaaagtatt
     8041 ccaataagta aatatagagt taaaggcttg aaacttgcta taactaatat attaggggat
     8101 atagtatgct aacaaatcta agtataggat tttggaatat tattaattta gtttcaggta
     8161 ctatagattg gattatattt tttttagtaa tccacttagt tggaaagagg aaaatatcta
     8221 atataagata tatatttaca acaataatta tatttatatt tatggcatac ataaatgtag
     8281 ttaaagtgtt tccaaatatt aagattcttc tatgtatggt aataggaata tcattttata
     8341 taacatcata tgaagataaa atatataaaa gtataatagt tagtttattg ttttggctag
     8401 gattaatgat tgctgaaggt tctgcagtag gtctagtagt atttataaat aaattaaata
     8461 atattaacat aacacaacat gaaaacttat ttagaataca agctattatt atgtcaaagg
     8521 tatttttatt tataggtttt atattgttta agtattttaa attatcttta gattttaaac
     8581 ctaaagatat gatactaata ggaataccta tattatctaa tatagttagc ttattactta
     8641 tatttgaata taatttaaga gtcaatacaa ttactacaga aaacttattt atatttatat
     8701 ttgcaatttt attgatttta tcatcaagta taatactttt aatagtaata gggaaaattg
     8761 tacaagacga taaattaaat ctagagtatg aacttataaa tgaaagaata aaaacaaact
     8821 acaggaatta tgaaaatata aacgaagttc atgatagatt aaaatatgtt tatcatgatt
     8881 tgaaaaacca tatgatttgt ataaaaaatt atgacactaa agaagaaatt atatcatata
     8941 tagataactt agagtttcag ataagtgaat ttgaacattt taaaaatact ggaaataaaa
     9001 cattagattt aattttagca gaaaaaataa gtatttgtaa aaagtatgat attcaaattg
     9061 aagataacat aaatatatct aaattaaact ttattgaaaa taatgatata tgttcaatat
     9121 ttgcaaattc actagataat gcaattgaag catgtatgga tataaataat gagctagaaa
     9181 aaagaataga agttaaagct acatatataa ataaatttgc aataataaaa tttataaata
     9241 ctaaggtaaa tgatattaaa cttattgata aacgtattca aactagtaag gatgatgata
     9301 agatacatgg aataggactt gctagtatta aatatatagt aaataaatat ggtggtgaaa
     9361 caatagttaa ttactcagat aatgagttta tattaaaaat aatgatacca ataaaaagtt
     9421 aggagagtac aaaatgaaaa aaatagcgtt aaacttactt aaaaacatct cagcattaag
     9481 ttttgggata gctgtattat cagcaaacag tgcaagttct tgggtagcac atcaggcaaa
     9541 agagccacaa gctttacaaa aactaaaaaa ataacattaa taaaaggacc tcttcttatt
     9601 aaaggagagg ttttacatgt tgaggaggaa atatgtttaa aagattatca tataaatttg
     9661 ccaatatatt agttaataat gagattgtag aaagtgaaga ttttgaaata tatagatatg
     9721 gatttgaaac tttgatttat ttcatagtta atatttcggt agcattattt ataggaatta
     9781 tttttgatag atttatacat acagttatat ttttaagttg ttattgcact ttaagacagt
     9841 tcacaggagg ttatcatgct aggaattata aagagtgtac attaacattt gcagttattt
     9901 atttgataac tattttttca gctaataata tagatataaa taaatataag tatttattag
     9961 tattattaat gattattagt atactaacaa tttacaagtt agctccttta gaacatagaa
    10021 acaaacctct tagtgaaagt gagaaaaagc attatagaaa gactgtgcaa aaaatactat
    10081 tcgtaataat atgtttaatt attttatgta aaatacttaa tatttttcag caatatgtga
    10141 tttatgcaat aatttctata tattggattg ctatattaat atatattgga atgaaggtta
    10201 ataatgatca gtaagattat aaaacatttt ctaaattaat taatttcact aattaatatt
    10261 aattttaaaa aataatgtca aggtaaatat ttgaacttat taaatatagt tgaatctatt
    10321 gtaagttgta tcaaaggatt ggctaggagg ggcataaata tgagtagtat taaggttata
    10381 tcagacttta taaccaaaat aattagaaaa aatattttat ttgcaataac agttattatt
    10441 ataaattacc atctcataca acgatggaca catatatcca atagaggaat ttcatttaat
    10501 gattacttat ccttgagtgt atatacttgt atgacaatta gtattttaac catgttaaat
    10561 gtaatttttt atatgagaaa aagttatgat actgtaaaaa tattatgtag aaataatttt
    10621 gattattttt taggtatttc tgttgctgta actagaaaca actttatatt ttgttgcata
    10681 ccaattatat atgctatagc atactcaagt ttaataagaa cattatcaat tgtaacgatt
    10741 aaatcaacta tatttatatt ggtatttgaa tggtttttac cacttactat tttatctata
    10801 gtgatatcat ttatttcagt tattataaat aatttaatac taaagattac cttatcatca
    10861 gtatttttat atttaacatc aactaagatg ttggatagag ttattgatat taaagaacca
    10921 ttttttaaag cattatttaa atcacttaat atatttgaag atcaatcata tgcttactat
    10981 tctgattttg tagggaaagt taataattca gcatatgtta tagataagat tatacctaca
    11041 ctatttgcaa ttactttaat aatagtagca tatctgatat taaatgatat tagaaagaaa
    11101 cagattaaat cattaatagt tatagtagtg tacagtattt tatttattgg attaaattat
    11161 atatcaatag atatgtcaac atatagagat gaggaacgta gttatggaga gtatttaaag
    11221 aaatctaata ttagtgatat taaaataaaa tcgcatgata tgaagattga ctttaaaagc
    11281 aagtcaaata taaaagatag aattgtttta ttaaacacat caaatgaaga gttaacaaat
    11341 attaatatga ttttagatga aatatttgat ataaaatata taaaagtaaa ttcggaaaat
    11401 ataaaattta acaaaaaaaa ggatttaatt gaagttaatt taaaggaaag tttagagcct
    11461 aatgacgagg tgacattaga tatagagtat gaaggctata ttaatgtgtt gacatcttgg
    11521 aatcaaaata aatatatagc tactaaagat gatattatgc ttccaccagg ttctttagcg
    11581 tggtacccaa aagtaaatca atcaagttgt atggcttata aaataaattt aagttctaaa
    11641 aataatgtct attcaaattt acctttactg aataagacta gtaatttttt aaaaaaagat
    11701 tatatatttg aaggaaagtc aaatgatatt gcaatatatg caggtgagtt taaagaagag
    11761 gtaaaagatg atataaaaat aatctatcca tcagatagta atgtaaatat ggatatagag
    11821 gaaaaaatta aatttttaat tgaaacaaga aatgaaatat taggtgatag caaagcatct
    11881 gtagatgcta aaaaatatgt agatgaagta gataatgtta tggaatcaat aaaaagaaaa
    11941 aaaattaata tgatattagt tgctgactta cctagcaatg aatttttata tgaagactca
    12001 aagggagaga aaattagcat aactatttat cattggcttt caggaaatat ccttataata
    12061 gataaataat aaaggaggtt aatatgataa acaagttaga aataagaaat ctaaatttta
    12121 attacaaaaa taaaaaagct ctggataata taaatttaac cctaacagat ggagtagttg
    12181 cattattagg tcctaatggt gctggaaaat caacattaat gaggttatta gtgactcttt
    12241 atgaaacttc aattggagag atagaattaa ataatataaa gtatagcaaa aacaatgaga
    12301 aaatcaaggc aaatgtagga tatatacctc aagattttga tatgtataat aatataaatg
    12361 ggcaagaata cttagagttt gtagctaaaa tgagaggtgt atctaagaat gatttaaaga
    12421 aacatataca aaaagtagta tcaaaagtta atctggacaa gtttataaat aataaaatag
    12481 gtacatactc aggtggtgta aagagacgac taggtatagc acaggcttta ataggtgatt
    12541 ctaagttaat agttatggat gaacctacag ttggtcttga tccagagcaa aggaatgaat
    12601 ttagaagatt gttaccagaa ataagtaaaa attctatagt attaatttca actcacatag
    12661 tagaagacat acaatttaac tgtgacaaat taattatatt aaatcaaggc aaaatattat
    12721 atgatggtac aattaataaa tttattgata tggttgaggt gtattcagta gtagtttcta
    12781 atgatgaatt tgaatattta gaacgtaata ttgatattat tgattataga agagttaaag
    12841 atggtgtgca agtaaagtat ataggtaaaa atacagagaa acttaaggat agcttaagga
    12901 tagatacaac tttacaagat gcttatataa actttttaca taaggagtta aaataaatgt
    12961 gtatattgga gttaataaaa tttgagctta aaaaaataga gcttaaacat gtgttaatat
    13021 ttgtaattat ttctatatta tgcatattta ttacaggcat attagattat aatgagttaa
    13081 tattttataa tgatgtacct ataatggata catattctat agcaggatgg ttaggaatta
    13141 taaagatatc agatgttttt ataccaataa tgtcagcaat cacaataatt tatgtgtttt
    13201 caaaagatta tttgggtaat ataaatgaga tacttacttt atataataaa aaaaaatata
    13261 atcaatttat agtatttaga tggtg
//
LOCUS       DAELXX010000107        12964 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.107, whole genome shotgun sequence.
ACCESSION   DAELXX010000107 DAELXX010000000
VERSION     DAELXX010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12964
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.107"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(63..1241)
                     /locus_tag="KPH29_002966"
     CDS             complement(63..1241)
                     /locus_tag="KPH29_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG1468545.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMFTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIVSIMIKIWLSRFNKFVGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKDMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
     gene            1613..1798
                     /locus_tag="KPH29_002967"
     CDS             1613..1798
                     /locus_tag="KPH29_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBG1468546.1"
                     /translation="MFFISDIYTKSPIKFDTPLQKEVYKILQKLDIDFERVDTDEAIT
                     MEDCVQINKKLNMKMVI"
     gene            complement(1863..4637)
                     /locus_tag="KPH29_002968"
     CDS             complement(1863..4637)
                     /locus_tag="KPH29_002968"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBG1468547.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAQALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGVIATLLCMFMIIQM
                     QRKGMLILDTSSILSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAMTSEGELNLIGNSRSYMKEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRSMNSNSKN"
     gene            complement(4809..6065)
                     /locus_tag="KPH29_002969"
     CDS             complement(4809..6065)
                     /locus_tag="KPH29_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBG1468548.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSAVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            complement(6137..7639)
                     /locus_tag="KPH29_002970"
     CDS             complement(6137..7639)
                     /locus_tag="KPH29_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBG1468549.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            complement(7730..8920)
                     /locus_tag="KPH29_002971"
     CDS             complement(7730..8920)
                     /locus_tag="KPH29_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG1468550.1"
                     /translation="MHELILISEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIEYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEN
                     IVCEIARGYQATAKLDYLFDLPAVINSKEMYTIACKSVCDLYSEDAIVDPIPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KNKI"
     gene            complement(9159..10247)
                     /locus_tag="KPH29_002972"
     CDS             complement(9159..10247)
                     /locus_tag="KPH29_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468551.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCRKKSTQSQE"
     gene            complement(10532..11230)
                     /locus_tag="KPH29_002973"
     CDS             complement(10532..11230)
                     /locus_tag="KPH29_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBG1468552.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIE
                     KGMTPKVYKLNTKN"
     gene            complement(11344..12849)
                     /locus_tag="KPH29_002974"
     CDS             complement(11344..12849)
                     /locus_tag="KPH29_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG1468553.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
BASE COUNT         4240 a         2305 c         1457 g         4962 t
ORIGIN      
        1 atgcctctat actattgtct atctttttta cattttttac cttttatgag attaattaat
       61 aattaaataa aatccttatc tacaacaatt acacaattat attcaggatt tatctcctta
      121 actaattttg ttatatcttc ttttaagtca tcctctgaca tgtctttgga aaattcggaa
      181 ggattaacta ctatatcaaa tattaaattc ttcttgcttc cttttccaac tattctaaaa
      241 tcatgcatag attttacaag actatttttc ttaagtattt cttcaagttc ttttttggct
      301 gacataactt ctttatcttc aagacatatt gggtccatgt gaattactag atatatatct
      361 aatttttcag atatttctct ctctgcagta tctataattt catgtatttc cattatatct
      421 atgttggatg gaatttctgc atgtatagat gcaatacatc taccaactcc atagttatga
      481 ataattaaat catgtgtacc tattatatat ttataagata acaatagctc tataatagaa
      541 tttaccattt cttcatccgg tgcttctcct aaaagaggat ttatagtatc ttttacaaga
      601 gagaaaccag cataaagtat cactaatgat actacgatac caacatatcc atctatagga
      661 aaattagtaa atctagctac aataaatgat ataactacac aacttgaagt aaatacatct
      721 cctaaagcat caagtgaaac tgcttttaag gctgaagaat caattttatt tcccacaaat
      781 ttattaaaac gactcaacca gattttaatc attatagata caattaaaag tataagtggt
      841 attacttcaa attttacagg aattggattt actatccttt ctatagatga ctttacaaat
      901 tgaagtccaa ccaacatcac cataaatgct actattaatg ctgataaata ctctattctt
      961 ccatgaccaa aaggatgttc tttgtcagct ggcttacttg ctaatttaaa acctatcata
     1021 gttattgctg aagatgccat atcagaaaga ttattaaatg catcagccat tatagctata
     1081 ctagatgtga acattcctat gaataccttt atcacaaata aaagtaaatt ggacaatata
     1141 cctactacac cagctacata accatactta tttctaacat ctgtattttg aacgttttcg
     1201 ctatctctga taaatgtttt gactaggatt ttggaaaaca tgaaacttac ctccctgccc
     1261 tttattttat taacaaattt tataatctat atctttattt atgataacat aatttatata
     1321 taaaattctt aaaaatcact ctacttcagt aaaatattat tctatatatc tatttagata
     1381 caaattttat atttcgtaaa gtttttatgg cttagtgtct gtttaataga ttctatataa
     1441 gtttttacaa agattattta ttatgataat atcatttttc ccaagtatta cttagaatat
     1501 gatttttttg ttttgtcttt acatatttta aatttatact atatttatgt ttttataacc
     1561 ttatgataca ctataactat aagattttaa acggaaagga atgaaataaa tcatgttttt
     1621 tatcagtgat atttacacaa aatcacctat taaatttgat actccattgc aaaaagaggt
     1681 atacaaaata ttacaaaagt tagatattga ctttgaacgt gttgacacag atgaagcaat
     1741 tacaatggaa gattgtgttc agattaataa aaaattaaat atgaaaatgg taatataaaa
     1801 caacattaaa caaacaaaaa ccgcaaagtt acaatatata gtactttgcg gtttcataag
     1861 aattaatttt tactattaga gttcatactt ctactatttt tcattgttct aagtattagc
     1921 tttacaaatt catttgacaa taatatagtg gatgcaaata gtattatttt tatccacata
     1981 tcaaagtcca aagaaacaga attaaagaaa ctctggaaca cttgtgtaaa tattatttga
     2041 attatccctg taactactat tatttgcaat gccagtttat ttttaaagaa atttggtatt
     2101 gtactattta aaccaaattc tctacaattt aatgcattaa aaagggcact gaacgcaaat
     2161 aaagaaaata taacagtacc ttgttcttct gatgtagctc ccaatatatt aaaagctgat
     2221 tgagtaaata caattgtaat tataagaata gcatttataa taatatttac aaacatagac
     2281 ctagctatta ttccagaatg tctatttatt ggttttcttt tcaatacata atctctaaca
     2341 ggttctaatc ctaatgctag ggctggagga ccatccatta ttatatttac ccataataat
     2401 tgtattgtag taaatggcat atccttacca gtaagttgag atattatagc aattataaat
     2461 gctactatat ttacagtaag ctgaaactgt ataaatcttt gaaagttctc atatatacct
     2521 cttccccatt ttattccctc tactatagta ctaaagctat catcagtcaa tattatatct
     2581 gctgcatttt tagatacttc tgttcctgaa atacccattg caattccaac atctgcttga
     2641 ctaagagctg gggcatcatt tataccatca cctgttacag ctacaacttc gccacttttt
     2701 tgaagcgctg aaactattct catttttgta tctggtttac ttcttgcaac aatagatatt
     2761 cctttaattt cttctctcag ttctttatct gtcaatacat ctatatatgt tgcttcaact
     2821 gcctttttcc catcatttaa taatcctaat tcatttccaa tagccgttgc tgtatttata
     2881 ttatctcctg taagcatttt tacatctact cctgcattaa atgctttatc aatggaatct
     2941 ttgacccctt ctctcaatgg gtccactatt cctacaaatc cactaaaaac taaattatct
     3001 tccttcatat aacttcttga atttcctatt aaattcaatt caccttctga agtcattgca
     3061 acttctgttt tagaattact cattttttta taagcaaatc ctaatgttct catagattta
     3121 atttgtaact tcttaatctc atctaaaata cttttttcta cttttggagt tattggaaca
     3181 atattttttc cttgttgtac ataagaacat ttttttaata atacttctgg agcacctttt
     3241 gaaagtaata tatctgaatc ctcttgacga attaatgttg acatcttctt ttcttctgaa
     3301 ctaaatggta tttgtgatat caaatacgtt tgttttctca tttcattata gttcttatca
     3361 ttatgatata aaagtaatgc acactctgtt gcacttccta tatatttaaa tgaattatct
     3421 tctttttcta tatctgcagt tgaatttact atacagtttt gttcaaaata gctgttactt
     3481 tgatactctc ctccacttat atatttacca tcaacatatg ctacctctac catcattttg
     3541 ttttgtgtta aagtccctgt tttgtcggaa catattacag aaacagaacc tatagtttca
     3601 caggcttctt tctttgttac taatgcattt atctttgcca tcttttgcat tgttatagct
     3661 aaggtaatat ttatcatagt tggcaaacct tctggaacag ttgcaactat taatgcaata
     3721 caaaccatat atgcattttt agcaggttct aatgattgta agaaagataa tatactgctt
     3781 gtatctaaaa ttaacatacc ttttctctgc atctgtataa tcataaacat acacaataaa
     3841 gtagctatta cgcctgatat tcctgctatt ttagcaccca acttccctaa tttaatttga
     3901 agtggagttt gctggtcttc atttgataaa ttctgtgcta tcttacccat ctctgtctta
     3961 tcaccagtat gagtgacaac cattatgccc ctaccatatg ctacaagagt ccctccaaat
     4021 accatattga cttgctttgc aggtattgca tcctgttcga ttatttctgt tttagaataa
     4081 actacttcca tatctaatac agcatcagca ttcttagcta catcatcaga ttctcctgtt
     4141 agcatatctt ctctaagttt caaatttata gactgtataa gtcttccatc tgctggtatc
     4201 atatctccag tttctatata tacaatatct cctggtacta aatcattttt tgatatttta
     4261 attatctttc catttctaag tacttttact tctatatttt ctgttaattt tgacagggcc
     4321 tgagctgctt ttttagattt tccttcagtt attatgccta tagaaattcc aataaaaata
     4381 gcccctaaaa tacctaatgc atcatgtaat tctcccacaa aagaacttat tactgctgct
     4441 cctatgagaa ttaatatcat aggttctgtc aaactttctc ccaactcgtc ccaaaatgtt
     4501 ctaccttctt ttatagtaaa ttcatttaat ccatatctaa gcttcctttc ttctacttca
     4561 ttatcatcaa gtccaatttc tggatttgtt tttaagtatt ttaatacttc tttggtaggt
     4621 ttattataat acctcataac tttatccccc ttgttaatta tctataagga attatatgct
     4681 tatgatagct tgtatattac ttttcaaaaa aaataaggtg ttgattaatc aacaccttat
     4741 agtggttata tattattaaa ttttatgtta caggctttat aaattgtgag ctatacaaat
     4801 caaaatattt aatcttcaaa atacccttct ggatataatg ggaacttaga tataagttct
     4861 tgtgcctctg ctttacattc atctaatact tctttattat caatattttc tgctacctta
     4921 ttcattattc ctgctatctg tataacttct ttttctttca gacctctttg agatattgat
     4981 gtaagaccta tacgaacacc actagttaca aatggtgatt gtgggtcgtc aggtataaca
     5041 tttttattaa ctgttattcc tacatattca agagcttttt caaattgttt tcctgtaaga
     5101 ttcttacttc ttaagtcaac cattactata tgattatcag tacctccact tacaatacta
     5161 aaaccatgtg aaatcagttc agaagctaga gtctgagcat ttactacaac ttgctccatt
     5221 atctttttaa atttatctgt acttaaatat aatagtgaaa aggcttttgc tgcaactgta
     5281 ttaagatgta tagaccctaa tgttcctgga aaaacacctt tatcgagttt ctttgcatgt
     5341 tctgctttac aaagtacaat accacttctt ggtccacaga aagtctttgt cgtagatgaa
     5401 gaaacaaaat ctgcataagg tacaggacta ggtattactt tcgctgcgac taatcctgca
     5461 acatgtgcca tatctaccat aaagtaagct cctacttctt ttgcaacctt tgatattctt
     5521 tcataatcta ttaatcttgg atatgaactt gcccctgcaa ttataagttt tggtctacat
     5581 tcttttgcct ttgcttcaag agcatcataa tctatacgtc cagtatttgg gtctacccca
     5641 taaaattcat atttataaat gtcatgtaaa aaattaactg cgctaccatg tgtcaagtgt
     5701 ccaccttggt caagacgcat acttaaaaca gtgtcatttg gttttaatat tgatgaatat
     5761 acacaatagt tagctgtaga acctgaatac ggttgtacgt ttacatgttc tgctccaaat
     5821 acttcttttg cacgttttat tgctagggtt tcaagtttat ctgcttcttc agaacctgct
     5881 tgaaaacgag cccctggata gccttcttct gttttatttg taaatacaca tccacttaat
     5941 tcaagtactt ctgttggtgc tgtactttca gaagctatca tctcaatatt gtgttcttgt
     6001 ctaaccaact catcagctac aatcttgtat aactctgggt ctgaaatttt tgttgttttt
     6061 aacataataa tcctcctctt aattaaagaa tatattttat tagtttaatc ttcattgata
     6121 aatttattat aatggtctat aaaacgtaag atattcctcc accaggtcct aatggtatgc
     6181 caatacccat gaatagtaac aatattatta tttctattaa tgtaaatacc actgagtatg
     6241 gaatcatatt cgatatgaca gtacctatac ctatatcttt atcatatctt ctggcaaagg
     6301 ccaatataac tggaaaatac ggaaaaagtg gacttattgg atttgttgaa gcatctccaa
     6361 tacgataagc tatttgagtt aatgctggat taaagtctag tagcataaac atgggaacaa
     6421 atataggtgc taaaatcgcc cattttgcag aagcacttcc tacaaataaa ttgataaatc
     6481 cagatagcaa tataaatcct attatcaatc catagctagg tatccccgac tctgctagta
     6541 attttgctcc tgttattgat aaaattgttc ctaagttact aagattaaat aaattaataa
     6601 actgggatgc tataaatgct aggactatat atccacccat atcactcata ctactaccca
     6661 tcatagatac taaatctttg tcacttttta tcttcttact tacttttcca tacactaatc
     6721 ctggagttaa aaatataata gtgattatag gaactatacc tttcattaaa ggtgcattac
     6781 tagatagtat agaaccagtc tctggatctt tcataaatgc attgtcacct atacataagg
     6841 caactatcac tactactaca actaacaatg ataataaagc gtattttata cccttatttt
     6901 caagttcact caattcctgt atatccttat catttgttga atcattatat tttgtaaaac
     6961 gaggagctat aaatttttca gttacaaatg ctcctgcaaa agttaaaact atcgctgata
     7021 taaaagcaaa ataaagattc attgctggat tgccttggta agatgggtcc atcatttgag
     7081 ctgctggtat tgtaaatcca gctaacaata catctgtagt actaagcata acatttgctg
     7141 caaatccccc tgcaacacct gcaaaagctg caaacatacc tacaagagga tgtcttccaa
     7201 ttcctaaaaa cacaagtgct gcaagtggtg gtaatattat aaatcctaca tctgctgctg
     7261 tatgtgataa catacctgca aatacgattg ctacagttac aagtttttgt ggaactttac
     7321 ttataccctt tttcattaac acttccataa atccagattt ctcagcaaca cctgctccaa
     7381 gcattgtaac cagtactaat cctaaaggag caaatgactg gaagtttgta actatgctac
     7441 ctaaatatga ttgaatctgc tctttctcta gtagatttac tacctttaca acttcttttg
     7501 tagaaggatg tatttcttct actcctaaat tagatataac tgctgacaat attacaacaa
     7561 ttatacatag taataaaaat atggttactg ggtctggaag tttatttcct gctctctcta
     7621 caacatttag tgccctcatc aataatccac tatctttgtt tttttcctgt gaattaattt
     7681 tcctcaaact aataccccct cataaacttt atctccagaa tgctctatct taaattttat
     7741 tttttaattt atccatataa tttataacag actgacatag tacatttgta cctaccaata
     7801 tagaattttt atcagcttta aatttaggat tatgccactg atatatacaa tcttgttctt
     7861 tattgcctac ccctaaccag taaaaaaatc ctggggcctt ttccataaat atagaaaaat
     7921 cttctcctcc tgtagaaggt attggatcaa ctatagcatc ttctgaatac aaatcacaca
     7981 cagacttaca agctattgtg tacatctctt tactatttat aacagctggt aaatcaaata
     8041 aataatctag ctttgcagtt gcttgatatc cacgagctat ctcacaaact atattttcta
     8101 aattttctga tattttcttt cgtacttcat tactaaagct tctacatgta cctgtcatat
     8161 ctaccttttc tgaaattaca ttatttgcag tccctccatt tatagtacct attgatataa
     8221 cagctgattc taatggactt atgtttctgc ttactatggt ttgaataccc attataattg
     8281 cacttgatgc tactataggg tctattgttc tgtttggtat acctccgtga cctccaaatc
     8341 catttacttc tatcttaata gtgtctactg ctgccattaa cccacctaac ttaacaccta
     8401 tttttccaca aggtatatct ggatgattat gaagtccaaa tatagcatca acttttggat
     8461 tttccattac tcctcgttcc actagcatct tcgcaccttt attgacttct tcggctggtt
     8521 gaaatataaa ctttacattt ccatgaagac tatctctaat atgagacaat acatatgcac
     8581 atcctacaag gcaagatgtg tgtatatcat gcccacatgc atgcattata ccatcatatc
     8641 tggatttata ctcaatatca acctcttcat atattggcaa tgcatcaata tctcctctca
     8701 gtgctactgt gggaccatca tacttccctt ttaataatcc aacaactcct gtttccattc
     8761 ctaaatctaa tatttctatg tctaaactta ctaataaatc ttttatagct tttgtagtcc
     8821 taaattcttt aaaacttaat tcggggtgca tatgaatatc tgtaaaaaaa tctaaaatat
     8881 cttctttata cttttctgag attaaaataa gttcatgcat actaacacct ctccaaaatt
     8941 ttattttttt attttttata atttaactac aaattatata agttaataat ctttataaag
     9001 atatttttta taaatttaat tttagtttat atttatattt tactatatta ttttaattaa
     9061 atatattatc atgttttgca atttatttaa ctattttgac tttttaaatt cggattatat
     9121 aaaaaatttc tttaaataaa aactattaat aggtgactct attcctgact ttgtgttgat
     9181 ttttttctac atgtaattgc tttatacacc atacagacta aaattaaaag ccccatataa
     9241 cctgtatatg gatataaaat gtttactaat ttaccaaacg gtaaaaaacc acctatacaa
     9301 gccaaaatag ctattactgt tgttaccaat ttaaatttag gatgttcatc ttcaacaatt
     9361 ctattggtta cagaccataa aagtggaaca gctgttgtat agatacctaa tattaatgca
     9421 actgaaaaac atacacctac aataggagat atattatctg ctaaataaag tgatggtatt
     9481 tctttcatat acaaatttcc tatatctgaa atcatagcca aatacataac tataccagca
     9541 gtcataaaca ctattccacc taataaacct ccccaaaaag catccttttt actttcaata
     9601 gaagctccca ttcctgttaa aaaaacaatc attacaataa tatttaatgt attatatatt
     9661 acaccagaaa cgattggact ttctgatgct tttgtaactt caatattttt catagcttct
     9721 ccaaccatag ataaaccatc aaaatgctta aacaagctta ttaatccaac agcaattgta
     9781 aatactgcta tcactggtcc aatacgtcca agtatgctta ctaatttttc taaacccata
     9841 gacactgtaa ttaaagtagc aacagccatc aatactcttc ctaagtaagg atttatacca
     9901 tagtattcag ttaaagttga gcctgcacct gaaatcatta ctataagaat cccaaataaa
     9961 aatattggac taaaccattc gtaaaatttt cctaaaatct tcccacaata aaaagtgtaa
    10021 attcttgttg gttctttaag ttttaattct ctacctctag ttataacttc agcaccaaga
    10081 aatgaaaaca ataccattga tattaatgct ccaattaaac ccatataccc ttgtgaggca
    10141 aaaaattgta gaatttcttg accagtagca aacccagaac caattgctgt tgctagataa
    10201 gcgcctgcaa tacagataat atttttcata ttcactttgt ctgacatatt aaattacctc
    10261 cattataaag ttaccttcct ttaagaaata taaactctca gtgaattaaa tgaaaattct
    10321 gtctttttga aacagtattt aattcacttt ttttattttg tatatctttt tctttttatt
    10381 tatatatatt tttttatata aataggtaca gcttttataa ggtttaatat tattaaatat
    10441 gtttaaagcg ctttaatttt tatcataatt aaagtatact tattcagaca cactcaaaca
    10501 attatctata ttgacttttt atttatcaat ttcaattttt agtgttcagt ttataaactt
    10561 ttggagtcat tcctttttct atcttaaagt ttttataaaa gttatttagg ctattatatc
    10621 caactatttc tgcaatctca tttatacttt ttgtagtatt ttcaagtaat tcaatagatt
    10681 tttctattct tatatgattt agatatgata taaaagttgt atttaaatta cttttaaaaa
    10741 tcctagatat attagatgga ttggttaaaa ataatctagc acaatcattt aactttaagt
    10801 cattgctata gtttagattt atatattcca gtatctcatt taataaatca ttattggtat
    10861 attgatatcc aataattttt tctacagact tagtaaattc tttcatatcc aatggtttaa
    10921 ggagtatatc ttttacattt aatctcaaag ctttttgagc atattcaaaa tagttaaatg
    10981 cagttattac tacaaagttt atttttttag ataaatgaga tgttttttct attacttcta
    11041 acccattgta tattggcatt tgaatatcta taaaaactat gtctggctct aaaccaatga
    11101 ttttatctac tgcttcatca ccagatgttg ctttaccaac aatttttatt ggcatatctt
    11161 tcatttctat aaaatgtgtt attattttta gaacagcttc ttcatcatct attattagtg
    11221 ctcttctcat gagaatacct cccttttagc ttaataatat aaaaaagtaa gatatataaa
    11281 acaacataaa aacatctaat atatttctta acatttataa aacttattaa tttttcacaa
    11341 gttctataca tactccttat aattttcaat aggaatttta atcattatgt aagttccttc
    11401 attatcactc gactttatat ccatatcaaa attatttcca taagcagatg tgaattttgt
    11461 atatattaaa gacaaaccat gaccattatt actacgaata cctgccttta ttttgtctat
    11521 attatttcca tctaagatag ttccattatt atgaatctct ataatagcat actgagaatc
    11581 taatcttgca attatttcaa ttcgcttttc tgtgtcaata tctctaaatc catgtgtaaa
    11641 tgcattctct acgataggtt gaataaagtt aaaaggcaca gaaaggttat ataacttctc
    11701 tggaataaga tattttactt taagtgcatc tccataacgt aatttctgta aattcaaata
    11761 atttttaatg tataatattt cagattctaa ttctacaaat cttaaatcag acctcattgt
    11821 atatctaaac attcttgata aatctattat tgcactatac aaatcataac taccatcatc
    11881 tagtgctatg cttgccattg aatttaatgt attaaataaa aagtgatgat ttattcttaa
    11941 attagtaacc atatcttgag ctacttttaa attcatttca agttgctctc cataatgata
    12001 tgttcttgct atagtcttct ctttttcttg taaatccttt cttatatcat taaaagagca
    12061 aaaatttatt atatttttag atatttgttt caagaggcgt tttatacttc tagcagctcc
    12121 ctctggaata tcatataaat tattatgctc tgtatctaaa tttaaatcag ataatagtac
    12181 ataccctcct cttataacac ctatgctatt gcttttgtac agtattggta tattatatat
    12241 agttatccca tattcacata caaattgatt gctacttttt tgattcatac aatcacaatt
    12301 ttgaggaaat ttcataggat tacatttttc aaaacaaaaa agtggaaaat aagggttttt
    12361 taaaactata ttccatgaat catcatatat aattaatgga attttaaacg ggtctataaa
    12421 ttgaccacga attgattcta ctgctgcata caaatttaat gtactttcac tataatttat
    12481 caaggtcttc tttaaataat tattaggatt aatatctata acttcactct ctcctacatc
    12541 atctacatta tttgaaacaa taagttcttt tatttctcta tcgcctgtat ttatagtttc
    12601 atgtgtgcta ccagattcta tataaaaaat cataccttga taaaatggct tccaaactcc
    12661 atttataata tacaaaccct ctccctctaa aatatatatc atctgctctt gaccataatg
    12721 aacatgatta aaatgtttct taccaggcat agttactgca atgtatatat tcatagcttg
    12781 ttttggatta ttttcatcat aagtatgttt ccattcaata tatccccact cagtattttg
    12841 aattttcata aaagattcct ttcctgtaaa ttatttatgt tcatattgtg aaaaattagt
    12901 aaaaaaaatg tgtacatact ctttcatatg atagcttact atatcaatat tcattagttt
    12961 gtac
//
LOCUS       DAELXX010000108        12564 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.108, whole genome shotgun sequence.
ACCESSION   DAELXX010000108 DAELXX010000000
VERSION     DAELXX010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12564
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.108"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            89..2434
                     /gene="secA"
                     /locus_tag="KPH29_002975"
     CDS             89..2434
                     /gene="secA"
                     /locus_tag="KPH29_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBG1468554.1"
                     /translation="MSVLDAILDKADEQEIKKLNLIVDKIESLEKDMEIKENEQLKEM
                     TNTFRLRLDKGESLDDILPEAFAVVREVSKRVLGMRQYKVQLIGGIVIHQGKIAEMKT
                     GEGKTLVEVAPVYLNALTGKGVHVITVNDYLAERDKELMRPVYEFLGLTVGVILSNQD
                     PSIRKEQYKCDITYGTNSEFGFDYLRDNMVPDLSHKVQRELNFAIVDEIDSILIDEAR
                     TPLIIAGDGDEDLKLYELANNFVKTVKEEDFEMDRKDKTIALTASGISKAESFFGITN
                     LTDIKNIELYHHINQALRGHKLMEKDVDYVISNGEVMIVDEFTGRVMDGRRYTDGLHQ
                     AIEAKEGVEIKNESKTMATVTYQNFFRLYEKLSGMTGTAKTEEGEFESIYKLNVVQIP
                     TNRPVIRADLHDKVFKTEEEKYSAVVEEIIRIHKTRQPILVGTVSVEKSEKLSKMLKK
                     QGIKHQVLNAKQHDKEAEVISKAGKLDAITIATNMAGRGTDISLGAGDKEEEEKVKNL
                     GGLYVIGTERHESRRIDNQLRGRSGRQGDPGTSRFFVSLEDDVIKLYGGKTIEKLMKR
                     TSSKENTAIESKALTRAIERAQKGVEGKNFEIRKNVLKYDDTINEQRKVIYNERNKVL
                     NDEDIKEDIQKMVKDIIQEAGETYLIGRKRDYYGYFKHLYSTFMPADTLLIPGVDKKS
                     VQEIIDSTYEISKRVYDLKKMMIGIDKVAELEKTVLLKVVDQYWIDHIDAMEQLRQYI
                     GLKSYAQKDPFKEYALEGYDMFEALNKNIREATVQYLYKFN"
     gene            2849..4720
                     /locus_tag="KPH29_002976"
     CDS             2849..4720
                     /locus_tag="KPH29_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp2"
                     /protein_id="HBG1468555.1"
                     /translation="MNKKNLSVIMAAAMISTSVAPVFAAETTQVKKETITKKEATELV
                     SKVRDLMSQKYTGGSQVGQPIYEIKVGETLSKLKIITNIDELEKLVNALGENKELIVT
                     ITDKGHITNSANEVVAEATEKYENSADLSAEANSITEKAKTETNGIYKVADVKASYDS
                     AKDKLVITLRDKTDTVTSKTIEIGIGDEKIDLTANPVDSTGTNLDPSTEGFRVNKIVK
                     LGVAGAKNIDDVQLAEITIKNSDLNTVSPQDLYDGYRLTVKGNMVANGTSKSISDISS
                     KDSETGKYKFTIKYTDASGKAIELTVESTNEKDLKDAKAALEGNSKVKLIAGDDRYAT
                     AVAIAKQTKYTDNIVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKDI
                     IKKSPSAKIYIVGGESAVSNTAKKQLESVTKNVERLAGDDRHMTSVAVAKAMGSFKDA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLSADAIKLMDGKEIGIVGGSNNVSSQ
                     IENQLADVDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKADITDSQRNALSKKLNLGAEVTQIGNGVELTVIQKIAKILGW"
     gene            4827..5534
                     /locus_tag="KPH29_002977"
     CDS             4827..5534
                     /locus_tag="KPH29_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBG1468556.1"
                     /translation="MVDATLFFTPHQDDETLSMGSAIIEHVEKSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDVHKYSLSESEFSKDRDEEFKDSCEAMGVKESNIHIEDNRAH
                     DGELSKEKAREIILKYLEEYPDAKVKTVTPFKASGIHEDHRALGEAALELYKEGKIKD
                     LRFYVEPYDYKDFKKVNPNVEVWKVLPSQEEKLLSAMNAYKKWNPESGHYAIGYHSVK
                     SHFDELAINKIQYVHAP"
     gene            5567..7402
                     /locus_tag="KPH29_002978"
     CDS             5567..7402
                     /locus_tag="KPH29_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG1468557.1"
                     /translation="MKISKKIVSLLTMTFLTVTLYGNTSNASTKDTLTGSGRWETAIK
                     ISQAGWKKSENAVLVNDNSIADALSATPFAKAKDAPILLTQSNKLDSRTKAELKRLGV
                     KNVYLIGGSIALSSEIEKQLNAENISFERISGNSRYDTSLKLAEKLDREKSISKIVVV
                     NGEKGLADAVSVGAIAAQENMPIILSDSENGTEVADNFIDSKDIEKSYVIGGTYSISN
                     SVERSLPNATRIAGSSRSETNAKIIEEFYKDTDIKNIYVTKDGTRSKHDLIDSLAVGV
                     LASKNGSPILLAGNKLDSSQKDVLNTKIIDKVIQIGGLGNENVVEDVIDMQEETKYTV
                     ETIDELNVAIKKSDANDIIRFKPEKDKNITNSFKIETNKAITIEFDGIYKQSITIDMP
                     NGDIKNFGDIDNDLVINNIKNETLVNEGSIQGIDVYDENGCKIENEDSGEIWLITILK
                     NSKDVYILNRGEITKISNNSSSVTIKNSGNINTISGSEEPSISGKKPITNDTDKDEDK
                     KARGLYPKVESCIPALKDYVRITISTKPESSKYKIYYRVVDEKPSAKYVGEKISTKGW
                     SMVTISNQSFIERAKDGSYIEAVEIDTSNNEVSRWGKSSITDDGI"
     gene            complement(7450..7845)
                     /locus_tag="KPH29_002979"
     CDS             complement(7450..7845)
                     /locus_tag="KPH29_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBG1468558.1"
                     /translation="MILKKHRETILYLFFGAFTTLVNIVSYLFFTRVILFNFMVANAL
                     AWILAVLFAYVTNKFFVFESKRIEIRFVFKEFLSFVSFRLLSGVVEMLIMYVMIDLLF
                     VNDVIVKIFTNIVVIVLNYLFSKMIIFKK"
     gene            8072..10483
                     /locus_tag="KPH29_002980"
     CDS             8072..10483
                     /locus_tag="KPH29_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp84"
                     /protein_id="HBG1468559.1"
                     /translation="MRKYKSKKLSKLLALLTVCFLIVSTIPVSAENHKTLDGVETAEY
                     SESYLQYLEDVKNGDTAKYNGVIPFPHEMEGTTLRNKGRSSLPSAYKSSVAYNPMDLG
                     LTTPAKNQGSLNTCWSFSGMSTLEAYLKLKGYGTYDLSEEHLRWWATGGKYGWNLDDM
                     SGSSNVTAIGYLTAWAGPKLEKDIPYNLKSEAQGATKPSNMDTAPTQFNVTDVVRLNK
                     DKETVKNAIMQYGSVTSGYAHYSTYFNKDETAYNCTNKRAPLNHAVAIVGWDDNYSKD
                     NFASDVKPESNGAWLVKSSWGEFNSMKGFFWISYEDKTLLTDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETD
                     IDGYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISIGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNESAFNELN
                     TLLGK"
     gene            10780..12357
                     /locus_tag="KPH29_002981"
     CDS             10780..12357
                     /locus_tag="KPH29_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HBG1468560.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSSNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKGNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTNSVSR
                     AVEKSLPNAERVSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSKTLTVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVENLEAKVDSIEKAIDSISQKVN
                     KIQDILDKIGFLKKFLS"
BASE COUNT         5232 a         1407 c         2282 g         3643 t
ORIGIN      
        1 attatactat acaataaaat aattgtctac tattctataa ttttaaggta taataataat
       61 tgtatgataa aagaggaggt caaaaaatat gtcagtttta gatgcaatct tagataaagc
      121 ggatgaacaa gaaattaaaa aattaaattt aatagtagat aaaatagaat ctttagaaaa
      181 agatatggaa atcaaagaga atgaacaatt aaaagaaatg acaaatactt ttagacttag
      241 attagacaaa ggtgaaagtt tggatgatat acttccagaa gcatttgctg ttgttagaga
      301 ggtctcaaaa agagttttag gaatgcgtca atataaagtt cagttaattg gaggtatagt
      361 aattcatcaa ggaaagatag ctgagatgaa gactggtgag gggaaaactt tagttgaggt
      421 agctccagta tatctaaatg ctttaacagg aaaaggtgta catgtaatta cagtaaatga
      481 ttatttggca gaacgtgata aagaacttat gcgtccagta tatgaatttt taggattgac
      541 tgtaggtgta attttatcta accaagaccc aagtataaga aaagaacaat ataaatgtga
      601 tataacttat ggaacaaata gtgaatttgg atttgactat ttaagagata atatggtgcc
      661 agatttgtct cataaagtac aaagggaact aaactttgcc atagtagatg agatagactc
      721 aatattaata gatgaagcta gaactccact tattattgca ggagatggtg atgaagattt
      781 aaaactttat gagttagcaa ataactttgt aaaaactgtt aaagaagaag actttgagat
      841 ggatagaaaa gataaaacta tagcattaac agcaagtggt ataagcaaag ctgagtcatt
      901 ttttggtata acaaacctta ctgatataaa gaatatagaa ttatatcatc atataaatca
      961 agctttaaga ggtcataagc ttatggaaaa agatgttgac tatgttatct caaatggaga
     1021 agtaatgata gttgacgaat ttacaggaag agtaatggat ggtagaagat atacagatgg
     1081 acttcaccaa gctatagaag ccaaagaagg tgttgagata aagaatgaat ctaaaactat
     1141 ggctactgtg acttatcaaa atttcttcag actatatgaa aaactttctg gtatgactgg
     1201 tactgcaaag acagaagaag gggaatttga gtcaatctat aaattaaatg ttgtccaaat
     1261 accaactaat agaccagtta ttagagctga tttgcatgat aaggtattta aaacagaaga
     1321 agaaaagtat agtgctgttg tagaagaaat aataaggata cataagacta gacagccaat
     1381 actcgtggga acagtttctg ttgaaaaatc tgagaagtta tctaaaatgc ttaagaagca
     1441 aggtattaag catcaagtct taaatgctaa acaacatgat aaagaggcag aggtaatttc
     1501 taaagctggt aaattagatg ctataacgat tgctacaaat atggcaggta gaggtacgga
     1561 tatttctcta ggtgcaggag ataaagaaga agaagaaaaa gttaaaaatt taggtggtct
     1621 ttatgttata ggaacagaaa gacacgaatc aagaagaatt gacaatcagc ttagagggcg
     1681 ttctggtcgt caaggagacc caggtacatc aagatttttt gtaagtcttg aagatgatgt
     1741 aataaagctt tatggtggaa aaactataga gaaacttatg aagagaacaa gttcaaagga
     1801 aaatacggct attgaaagta aagcacttac aagggctata gaaagagcac aaaaaggtgt
     1861 agaaggtaag aattttgaaa taagaaaaaa tgttcttaaa tatgatgata ctattaatga
     1921 gcaaagaaag gttatatata atgaaagaaa taaagtgtta aatgatgaag atataaaaga
     1981 agatattcaa aaaatggtta aagacatcat acaagaagca ggagaaactt atttaattgg
     2041 aagaaaaaga gattattatg gatattttaa acatttatat agtacattta tgcctgcaga
     2101 tacattacta atacccggtg tagataaaaa aagtgtccaa gagataattg atagcacata
     2161 tgaaatttca aaaagggttt atgacttgaa aaagatgatg attggtattg ataaagttgc
     2221 ggagttagaa aaaacagtac ttttaaaagt ggttgatcaa tactggatag atcatataga
     2281 tgctatggaa cagttaagac agtatatagg tcttaaatct tatgctcaaa aagatccgtt
     2341 taaagaatat gctttagaag gatatgatat gtttgaagct ttaaataaaa atataaggga
     2401 agcaacagta caatacttat ataaatttaa ctaaaaaagt tcatgtaatt tttattaaat
     2461 gaaaaataaa atattttgat tctttatatt gtaattacaa ttaaataatt aaggaataca
     2521 aatgaaaaag aagctgaaaa tcagcttctt tttctgtgtg agaaatacgg aaaaaagaga
     2581 tgcttgaaat ttctttcttc catttaatat tatactataa agaagatgaa aagtctatta
     2641 gttataaact ggattattaa aaaaaattac aattttgtta cttttttgta tacataaata
     2701 ttaaaaaaat aaaatataag aaaaagttta tatcttttgg ttaattatta caataagtct
     2761 catttattga aataatatca aatatatatt ataattggta aataaggaaa aaataataaa
     2821 aatttgaatt ttttaggggg aaaataccat gaataaaaaa aatctttctg taattatggc
     2881 tgctgcaatg ataagtacat cagtagctcc agtttttgct gcagaaacta cacaggtaaa
     2941 aaaagaaaca ataactaaga aagaagctac agaactagtt tcgaaagtta gagatttaat
     3001 gtctcaaaag tatactggtg gttctcaagt tggacaacca atatatgaaa taaaagttgg
     3061 cgagacttta tcaaaattaa aaataataac taatatagat gaattagaga aattagtaaa
     3121 tgctttggga gaaaataaag aacttattgt aactataaca gataaagggc atataacaaa
     3181 tagtgcaaat gaagtagttg cagaagcaac tgaaaaatat gaaaattcag cagacctttc
     3241 cgctgaggct aattctataa cagaaaaagc taaaactgaa actaatggaa tttataaagt
     3301 tgcagatgta aaagcttcat atgatagtgc taaagataag ttagttataa ctttaagaga
     3361 taaaacagac acagtaactt ctaaaactat agagataggt attggtgatg aaaaaattga
     3421 tttaacagca aatccagttg attcaacggg aacaaactta gacccttcta cagaaggatt
     3481 tagagtaaat aaaatcgtta aactaggtgt agcaggagct aaaaatattg atgatgtcca
     3541 attagctgaa ataactataa aaaatagtga cctaaataca gtttcaccac aagatttata
     3601 tgatggatat agattaactg ttaaaggtaa tatggtagca aatggaacat caaagtcaat
     3661 tagtgatatt tcatcaaaag attcagaaac aggaaagtat aaatttacta ttaagtatac
     3721 tgatgcatct ggaaaagcaa tagagcttac tgtagaaagt actaatgaaa aagatttaaa
     3781 agatgccaaa gctgcattag aaggtaattc aaaggttaaa ttgatagctg gagatgatag
     3841 atatgcaact gcagtggcta tagcaaaaca aacaaaatat actgacaata tagttatagt
     3901 taattcaaat aaactagttg atggattagc agctacacca cttgctcaat ctaaaaaagc
     3961 acctatatta ttagcatccg ataatgaaat accaaaagta actttagatt atataaaaga
     4021 tataattaag aaaagcccat cagctaaaat atatatagta ggtggagaat cagcagtatc
     4081 aaatacagct aaaaagcaat tagaatcagt aactaagaat gttgaaagac tagctggaga
     4141 tgatagacat atgacttctg tagcagtagc aaaagctatg gggtctttta aagatgcatt
     4201 tgtagtaggt gcgaaagggg aggctgatgc tatgagtata gctgccaaag ctgctgaact
     4261 taaggctcct ataatagtaa atggctggaa tgatctttca gcagacgcta tcaaattgat
     4321 ggatggaaaa gagattggta tagttggtgg ttctaacaat gtatctagtc aaattgaaaa
     4381 tcaacttgct gatgttgata aagatagaaa agttcaaaga gttgaaggag aaacaagaca
     4441 cgatactaat gctaaggtta tagaaacata ttatggaaaa ttagataaac tatatatagc
     4501 aaaagatgga tatggaaata atggtatgct agtagatgca ttggcagcag gacctctagc
     4561 agcaggtaaa ggtccaatac ttctagctaa agctgatata acagactcac aaaggaatgc
     4621 acttagtaaa aaattaaatc ttggtgcaga agtaactcaa ataggtaatg gagttgaatt
     4681 gacagtaata caaaagatag ctaaaatact aggttggtaa taacttatac tgtggagaag
     4741 attccttaat taatacagga atctttttct ataattatgc tatttattgt aaattataac
     4801 attttataaa gtaggaggat gtagtagtgg tagatgcaac tttatttttt acaccacatc
     4861 aagatgatga aaccttaagt atgggaagtg ctataattga acatgttgaa aaaagtgata
     4921 cacatgtcat attatgcaca gatggtagta aatctataat aagaaaagtt ttggatgatg
     4981 gagggtcatg ttcttatcat attaaagatg ttcataaata ctctttatca gaaagtgaat
     5041 tttcaaaaga tagagatgaa gaatttaagg atagctgtga agcaatgggt gtaaaagaaa
     5101 gtaatataca tatagaagat aatagagcac atgatggtga gttaagtaaa gaaaaggcaa
     5161 gggaaataat acttaagtac ttagaagaat atccagatgc aaaagtaaaa actgttactc
     5221 cgtttaaagc tagtggaata catgaagacc atagggcatt aggagaagct gcactagagt
     5281 tatataaaga aggcaaaata aaagatttaa gattttatgt agaaccatac gactataagg
     5341 attttaaaaa agttaatcca aatgtagagg tatggaaggt attacctagt caagaggaga
     5401 agttattaag tgcaatgaat gcatataaaa aatggaatcc tgaaagtgga cattatgcta
     5461 taggatatca ttcagtaaaa tctcattttg atgagctagc tataaataaa atacagtatg
     5521 tacatgctcc ataaaatatt atgaaagaat taggaggtaa gaattaatga aaatatcaaa
     5581 aaagatagtg tctttgttaa ctatgacatt tttaactgtt acattatatg gaaatacatc
     5641 taatgcatct acaaaagata cattaacagg ttctggaaga tgggaaacag caataaaaat
     5701 aagtcaagca ggatggaaaa agtctgaaaa cgctgtgcta gtaaatgata attcaatagc
     5761 agatgcttta tcagctactc catttgctaa agcaaaagat gcaccaatat tattaactca
     5821 aagtaataaa ttggatagta gaacaaaagc agaattaaaa agacttggtg tgaaaaatgt
     5881 atatttaata ggtggttcaa ttgcattaag ttcagagatt gaaaagcagt taaatgcaga
     5941 aaatataagt tttgaaagaa tatctggaaa tagtagatat gatacttctt taaaattagc
     6001 tgaaaagcta gatagggaaa aatctatatc taaaatagta gtagtaaatg gagaaaaggg
     6061 tcttgctgat gcagtgagtg ttggagctat agctgctcaa gaaaacatgc caataatact
     6121 ttctgattca gagaatggaa ctgaagtagc tgataatttt atagatagta aagatataga
     6181 aaaatcgtat gtaataggtg gtacatattc tatttctaat tctgtagaaa gaagtttacc
     6241 aaatgcaaca agaatagcag gtagtagtag aagtgagaca aatgcaaaaa ttatagaaga
     6301 attttacaaa gatactgata taaaaaatat ttatgtaaca aaggatggta caaggagtaa
     6361 gcatgattta atagattctt tagctgtggg tgtattagct tctaaaaatg gttctccaat
     6421 acttttagca ggaaataagc tagatagttc tcaaaaagat gtgttaaaca ctaaaattat
     6481 agataaagtt attcaaattg gcggattggg taatgaaaat gttgtagaag atgtaataga
     6541 tatgcaagaa gaaactaagt atactgttga aactattgat gaacttaatg ttgctataaa
     6601 aaaatcagat gcaaatgaca tcataagatt taagccagaa aaagataaaa atataactaa
     6661 ttcttttaaa atagaaacaa ataaagctat aactattgaa tttgatggta tatataaaca
     6721 atcaataact atagatatgc caaatggaga tataaaaaac tttggggaca tagataatga
     6781 tctagtaata aataatataa aaaatgagac tttagttaac gaaggaagta tacaagggat
     6841 agatgtttat gatgagaatg gatgtaaaat agagaatgag gattctggag aaatatggct
     6901 tataacgata cttaaaaatt caaaagatgt atatattttg aatcgtggag aaataactaa
     6961 aatatctaat aattcttcta gtgtaacaat aaaaaattca ggtaatatta atactataag
     7021 tggaagtgaa gaaccatcaa taagtggtaa aaaacctata actaatgata cagataaaga
     7081 tgaagataaa aaggcacgtg gtttatatcc taaagtagaa tcttgtatac ctgctttaaa
     7141 agattatgta agaataacta tatcaacaaa accagagagt tcaaagtata aaatttatta
     7201 tagagtagtt gatgaaaaac catctgcgaa atatgttgga gaaaaaatta gtactaaagg
     7261 ttggagtatg gttacaatat caaatcaatc ttttattgaa agagccaaag atggatcata
     7321 tatagaagca gtagaaatag atacctcaaa taatgaagtt tcacgatggg gaaaatctag
     7381 cattacagat gatggaattt agacattaaa cttattcatt ttgcagatta ctatttagta
     7441 atctgctatt tattttttaa aaattatcat cttgctaaac aagtaattta aaactataac
     7501 aactatatta gtaaatattt ttacaataac atcatttaca aaaagcaaat caatcattac
     7561 atacataatt aacatttcaa cgacaccaga aagtagccta aaactaacaa aagacaaaaa
     7621 ttctttaaat acgaacctta tttcaattct tttactttca aatacaaaaa acttattagt
     7681 aacataagca aataaaacag caagtatcca tgccaaagca tttgcaacca taaaattaaa
     7741 taaaataact ctagtaaaaa ataaatacga aactatattt acaagagtag taaatgctcc
     7801 aaaaaataaa tataatattg tctctctatg cttttttaaa atcaaatata atcctcctaa
     7861 tctaatttaa tataaatata cttaaagata ctaacaaaca ttagtatagc acatctatta
     7921 ataaatcaat atatcttaaa atattataaa aataaaacta taatattcta taattaatat
     7981 gtactcataa tccatatttt atcaaaataa aatatgacct taatttagaa atctgatata
     8041 ataaaaatta aatacatata agggggtaaa catgagaaag tacaaatcaa aaaaattgtc
     8101 aaagctactg gcattgttga cagtttgctt tttaatagtg tcaacaatac ctgtctcagc
     8161 agaaaaccat aaaactctag atggagtaga aactgcagag tattctgaaa gttatcttca
     8221 atacctagaa gatgtcaaaa atggagacac agcaaaatat aatggagtaa taccttttcc
     8281 acatgaaatg gaaggtacaa cacttcgaaa taagggcaga agtagtcttc catcagcata
     8341 taaatcaagt gtagcataca acccaatgga tcttggtctt actacaccag caaaaaatca
     8401 aggaagtctt aatacatgct ggtctttttc aggtatgtca actttagaag catatcttaa
     8461 attaaaggga tatggaacgt atgacttatc agaagaacat ttaagatggt gggcaactgg
     8521 tggaaaatat ggatggaact tagatgatat gtcaggtagt tcaaatgtaa cagccatagg
     8581 atatctgaca gcatgggcag gtcctaagtt agaaaaagat ataccatata atcttaaatc
     8641 tgaggcacaa ggtgcaacta aaccttcaaa tatggatact gcacctactc aatttaatgt
     8701 aacagatgtt gttcgtctta ataaagataa ggaaactgta aaaaatgcta taatgcaata
     8761 tggttcagtg acatcaggtt atgcacatta ttcgacatat tttaataaag atgaaacagc
     8821 atataactgt acaaataaga gggctccatt aaatcacgct gtagcgatag taggatggga
     8881 tgataattat tcaaaagata actttgcatc tgatgtaaaa ccagaatcaa atggagcatg
     8941 gttagtaaaa agcagttggg gagaatttaa ttctatgaaa ggattcttct ggatttctta
     9001 tgaagataaa actctactaa cagatacaga taactatgca atgaaatcag tatcgaaacc
     9061 agatagtgat aaaaaaatgt accaacttga atatgctggt cttagtaaga taatgtcaaa
     9121 taaagtaaca gcagcaaatg tatttgattt tagcagagat tctgaaaaac ttgactctgt
     9181 tatgtttgaa acagactctg taggagcaaa atatgaagta tattatgcac cagtagtaaa
     9241 tggagttcct caaaacaact caatgacaaa acttgcaagt ggaacagtat catattctgg
     9301 atacataaat gtacctacta attcttacag cttaccaaaa ggtaaaggag caatagtagt
     9361 agttatagac aacacagcaa atcctaatag agaaaaatca actttagcat atgaaactga
     9421 tatagatgga tactatctat atgaggctaa agcaaactta ggtgaaagct acatacttca
     9481 aaacaataag tttgaagata taaatacata tagtgaattt tctccttgta actttgttat
     9541 aaaagctata acaaaaacat cttctggaca agctacttca ggagaatctt taactggagc
     9601 agatagatat gaaacggcag ttaaagttag tcaaaaagga tggacttctt cacaaaatgc
     9661 agtattagta aatggagacg caatagtgga tgctttaaca gctacaccat ttacagcagc
     9721 aatcgactct ccaattcttt taacaggaaa agataactta gattcaaaaa ctaaggcaga
     9781 gttacaaaga ttaggaacta aaaaagttta tctaataggt ggagaaaatt ctttaagcaa
     9841 gaatgtacaa actcaactta gtaatatggg tatatcagta gaaagaattt caggtagtga
     9901 tagatacaag actagtatat ctctagctca aaagttaaat agtataaaat ctgtttcaca
     9961 agttgcagtg gcaaatggtg taaatggact tgcagatgca ataagtattg gtgcagcagc
    10021 tgctgataac aatatgccaa taatacttac taatgaaaag agtgagttgc aaggtgctga
    10081 tgagttttta aattcatcaa aaataactaa gtcttatata attggtggta cagctacttt
    10141 atcatcaaat ttagaaagta agctttcaaa tccaacaaga cttgcaggaa gtaatagaaa
    10201 tgaaactaat gctaagataa tagataaatt ctaccctagc tcagatttga aatatgcttt
    10261 tgttgttaaa gatggttcaa agagtcaagg agacttgata gatggactag cagtaggagc
    10321 attaggtgct aaaactgatt caccagtagt tctagttgga aataagttag atgaaagtca
    10381 aaaaaatgta cttaagtcta agaaaataga aactcctatt agagttggtg gaaatggaaa
    10441 tgaaagtgct tttaatgaac taaatactct tttaggaaaa tagtgaatgg aaagatagta
    10501 aggatataca ttttattatt aaaaaatatt caatagaagt ttataaatat aaaataacaa
    10561 aaaggggtgt ctcaaaatga actttttagt tcatgaggca ctcttttttg ttgttttata
    10621 tttttatcat ttaaataatg aaaaataggc ttgtctatat ttttagatag ttttcttatt
    10681 attgatgaaa aatgtaataa ttttaaactg taaaatagtg taaagttatt ttataatata
    10741 aatatagtaa gattttaaac aaaataaggg ggaataataa tgaaagcacc aaaaactatt
    10801 ttaacaatac tgacaatagc gcttacttta agtagcattt ctataatacc atcatatgca
    10861 cttacagagg aaaaattaat aggtaatggt agatatgaaa ctgctgtaaa gataagtcaa
    10921 aaggcataca gttctagttc taatgtagtg ttagttaatg acaattcatt agctgatgca
    10981 ctttcagcta caccatttgc aaaggctaaa ggagcaccaa tacttttgac agaaagtgat
    11041 aagctagatg ataggactga aaaagagata aaacgtctag gagctaaaga tatttatttg
    11101 ataggaggta ctgctgtact taataaagat atagagaaca agttaaaagg taatggactt
    11161 aatgtagaaa gaattaatgg taaggataga tatgaaactt cactgatttt ggctaataag
    11221 cttaaggata taaaagatat taaggaagtt gctgtagtaa atggtgaaaa aggattaagt
    11281 gatgctgtta gtgttggagc accagcagct caaaataaga tgcctataat tttgtctagt
    11341 cctaaggacg gagtagaagc atttgataaa tttataagag atgagaaagt tataaaagca
    11401 tacgtaattg gaggaacaaa ttctgtttca agagctgtag aaaagagcct tcctaatgca
    11461 gaaagagtga gtggaaaaga tagaaatgag actaatgcca aagttataga aaagttttat
    11521 actgatacaa atttaagtaa tttatatgta actaaggatg gtagtaaaaa tgagaatcaa
    11581 ctaatagact cgttagctgt tggagtattg gctgcaaaaa atgagtctcc tgttgttttg
    11641 gttggtaata aattaaacac gaaacaaagg gatatactta gtactaaaaa attaaatact
    11701 ataacacagg taggtggaaa tggaaatgaa gaagcttttg atgaaattaa gagtttacaa
    11761 gaaaaaactg tgtttgaagc aaaaactgtt gaggaattaa ctgatatgat aaatatagca
    11821 agtcctaatg atattataaa ctttaaacca aaagagaata ctgtaaatga agcttttaga
    11881 atggtaacaa ataaaccaat aactgtgaat ataaagggtg attgttcaaa gactcttaca
    11941 gtagatatgc ctaatggtga agtgaataac tatgctactt tggtaaatgt tatagttaga
    12001 aatattggag aaggtggatt taataaccat gatacaatca ctatattgtc agttcgtgac
    12061 aaaaatggta gagttataga aaatactaga aattcagata tagatacgtt gatgatatta
    12121 gcttctgcta atgatacaaa attgattaat gatggatata taggaaagct tatagataat
    12181 tcatctaata gtgatataac taataatggt actatagata aaaaagttaa tcaagttgag
    12241 aatttagagg ctaaagtaga ttcaattgaa aaagctatag attctataag tcaaaaagtc
    12301 aataaaatac aagatatact agataagatt ggatttctaa agaaatttct tagttaagaa
    12361 tatgtatatt taagtgaagt gtgtgttgta attgttctaa agttatatag ttaaacaggt
    12421 ttatatacaa acagacttat agataaatat gtagtttagt atatatttga ataagctttg
    12481 gggaggtaac atggggattg gaattgaatg taacaaaaaa ataaataaaa gtattgacaa
    12541 aactagaata aagaaaaata atct
//
LOCUS       DAELXX010000109        12339 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.109, whole genome shotgun sequence.
ACCESSION   DAELXX010000109 DAELXX010000000
VERSION     DAELXX010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12339
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.109"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            316..774
                     /locus_tag="KPH29_002982"
     CDS             316..774
                     /locus_tag="KPH29_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG1468561.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFILGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLRTLKSL"
     gene            835..1110
                     /locus_tag="KPH29_002983"
     CDS             835..1110
                     /locus_tag="KPH29_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG1468562.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            1169..2443
                     /locus_tag="KPH29_002984"
     CDS             1169..2443
                     /locus_tag="KPH29_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012951111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBG1468563.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPE
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            2573..3625
                     /locus_tag="KPH29_002985"
     CDS             2573..3625
                     /locus_tag="KPH29_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG1468564.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYVNIKATNVLPIGDMDFEDAAMIEPLAVAMHGVLNIGVQVGD
                     AVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDIDPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            3625..4656
                     /locus_tag="KPH29_002986"
     CDS             3625..4656
                     /locus_tag="KPH29_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBG1468565.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKDIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMASGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            4748..5560
                     /locus_tag="KPH29_002987"
     CDS             4748..5560
                     /locus_tag="KPH29_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBG1468566.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVNPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            5563..6483
                     /locus_tag="KPH29_002988"
     CDS             5563..6483
                     /locus_tag="KPH29_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBG1468567.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILASIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            6658..7104
                     /gene="rpiB"
                     /locus_tag="KPH29_002989"
     CDS             6658..7104
                     /gene="rpiB"
                     /locus_tag="KPH29_002989"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBG1468568.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            7143..7808
                     /locus_tag="KPH29_002990"
     CDS             7143..7808
                     /locus_tag="KPH29_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBG1468569.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            7918..9420
                     /locus_tag="KPH29_002991"
     CDS             7918..9420
                     /locus_tag="KPH29_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBG1468570.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTNIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNNSNLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRPYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
     gene            complement(9732..11324)
                     /locus_tag="KPH29_002992"
     CDS             complement(9732..11324)
                     /locus_tag="KPH29_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG1468571.1"
                     /translation="MVTKLKNLIINDSKAKFILLLLLFLLVSILFEAVYDLNNNFSYN
                     INCYYKEFALPYFYFFGTPVHLSKVELEFVCIGIILSSFLLTILCFTLYKKNKQTKVK
                     FLENVISLFSKVKIELTLILTLILLTLRFHAFTSNKMFYNTNLIIYNFIIISIVYIIY
                     ENIKYNNYNLKDMSITYVFFSNLLSMYNKKTINKKLAIVFLISIVVQAIIQYLVLIFF
                     MYIYYSEAIAIVILFTLVNTVFHFYIYNFLSVKFNYINNISKNIKNIENGNLKYKLEV
                     IGDDEISTLAKSINNITGGLETAVDANIKNERMKAELITNVSHDLKTPLTSILSYVDM
                     LKNNDFDRETINDYINILDKKSQRLKLLIDDIFEASKLSSGDVEFNITKTDIRELLIQ
                     SIVEFEDKIQNSSLDFIIETPEYAVFTMIDGKKTWRVFENLICNILKYSMPNTRVYID
                     MFIKEENIILTFKNISNDKLNLKPEELIERFRRGDISRKTDGSGLGLSIAQNIIELEN
                     AHMEIIIDADLFKVMLTFRRVN"
     gene            complement(11293..12009)
                     /locus_tag="KPH29_002993"
     CDS             complement(11293..12009)
                     /locus_tag="KPH29_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1468572.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSREIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
BASE COUNT         4883 a         1433 c         2144 g         3879 t
ORIGIN      
        1 ataaataatt ttataaaata gttgttaaac ataaaaaacc atttgtaaaa aacaaaaaaa
       61 tcatttctct aacaaaaaaa accacaaaaa atataaaaaa gtatggctag ttaagtgatt
      121 aacaagtaaa aattaaaata aaaatcaaaa aaaatttatc aatgaaagtc agctaaaatc
      181 ctggaagaga gcattatttt cgggaaaaca ttttcttttg tttcacaaaa aaagccactt
      241 tttggcacgc aacatgcttt atataaagtc aagaaaaact aaggtataag acaaaggcaa
      301 ttaggaggtg taaatatgga gtttataaat tttgtaagtg ataaagttat agaccttaaa
      361 atggattgta aaacgaaaga agatttcttt aaagaaatac ataataaagt ttttgattta
      421 ggctttgtaa aagaagaatt tggagaaaaa atattagcaa gagaaaatgt atttccaaca
      481 ggtttaaatt taggtgatta tggagtagca ataccacata cagatgcaga atatattaaa
      541 gagcaattta tatcagtctg tacttttaat gaaccagtag tatttagttc tatggaagac
      601 caagatgaaa aagtacctgt aaatttggct ttcatattag gacttaatca accacacagt
      661 caattatctg tattaacaga attaatggga attatgcaaa ataaagagtt agttgataag
      721 ttgatgagtt ctactgataa agaagaagtt ttaaggacac taaagtcttt ataaaaataa
      781 aaaagtacta aaaataaaaa atataaaaat aaaaattatt aggaggaaat aaaaatgaaa
      841 aaaatattag tagcatgtgg agcaggaata gcaacatcaa cagtagtttg tgacaaggta
      901 gagagattag ttaaggaaaa taatatacaa gcagaagtta tacaatgcaa aatagctgaa
      961 tgtgcaacaa agcaagaagg agcagattta atagtatcaa caactatact accaacaact
     1021 tataatatac cagcaataaa agcaacagca tatataactg gagtaaatac agcagcatta
     1081 gataaaaaaa tattagatgc tttaaaataa tcctatcata gatacattga aataatattt
     1141 tataaaacag aaaaaggagg atttataaat ggataaatta ttagcagtag tacaatacat
     1201 tttaaatatg ggaccgacag taatactacc aataacaatt gtagtactag gaatgatatt
     1261 tagaattaag tttacaaaag ctttaaagtc aggtcttaca attggtatag gatttgtggg
     1321 tataaactta gttgtaaacc tattaacatc aacactaggg ccagcggctc aaggaatggt
     1381 tgaaagattc ggacttaatt taacagtaat agatgttggt tggccaacaa cagcatcagc
     1441 aacatgggct tcaccagttg caccaatatt aataccagtt tgtttattag taaacttact
     1501 attattatac ttcaataaaa caaagatatt aaacgttgat atatggaatt attggcactt
     1561 tatagtagca ggtgcagttg gatatatagt aacaggaagt tatgtttggg ctatattctg
     1621 tgcaatacta atggagttat tagtactgtt tattgcagaa aagacagcaa aaaatgttca
     1681 ggagttttat gatttagaag gtatagcatt accaactggt tcaacagctt catttgctcc
     1741 acttggatat ttagtaggta aatttgttga aaaaatacca ggaataaata aaatacaagc
     1801 agacccagac tctatacaaa aaagatttgg tatatttgga gaaccaatga tgatggggat
     1861 tatactaggt ataatacttg gagcacttgc gggatatgat gtagcaggaa tattcaatgt
     1921 aggtatatca atgggtgcag taatgttact tatgcctaga atggttagaa tattaatgga
     1981 aggtttaatt ccaatttcag aatcaataag agatttctta caagcaaaat atggagacag
     2041 agaactttat ataggtctgg atgcagcagt agcaacggga catccagcag ttatatcaac
     2101 agctcttata cttgttccta taacgttgtt cttagcagtt atactaccag gaaacaaagt
     2161 tttaccattt ggagacttag cgacaatacc attttatatg gcatttatag tgtgctttag
     2221 aaaaggtaat atagttcaat cagttataac aggaacaata gtaatagcta tatcacttta
     2281 tatggctaca aactttgcag atgttcatac acaattatta caacaagcaa atatgatgcc
     2341 agaaggaaca acaatggtta caagtataga tacaggtgga aacttgctaa aatggggaat
     2401 attgaaatta tctcaattag tttcttcatt tggaatattc taatatgtag ttaatagaga
     2461 ttactctttt ttagtaaagt atgcgaaaat ttaacattta gcagctacta aaaaagagta
     2521 atatttttta taaattttta aaaatacaaa atacaagagg aggagattta tcgtgaaagc
     2581 agcagtatta catggcacaa atgatatgag atttgaagat atagaaataa aaccatgtga
     2641 aagtgatgaa gtaaaaataa aagttatggc agcaggaata tgtgggtcag atcctccaag
     2701 agtattaaaa cattggaaat atccagttcc agctatacca ggacatgagt tttctggagt
     2761 aattgctgaa gttggtaagg atgtaaaaaa tgtaaaagtt ggagatagag tagttgcaat
     2821 tccttttata ccttgtaatg aatgtgaata ttgtaaaaga ggattatttt cattgtgtga
     2881 tgaccatgga atgttaggag ctaaaagttt tggagctttt gcagagtatg taaatataaa
     2941 agctacaaat gtacttccaa taggagatat ggactttgaa gatgctgcaa tgatagagcc
     3001 actagcagtt gctatgcatg gagtacttaa tataggtgtt caagttggag acgctgtagc
     3061 cgtaatgggt agtggaacaa tggggcagtt ggtaatacaa ggacttaaaa tagcaggagc
     3121 aggaacaatt atagcagttg atatatctga taataaatta agagaatcaa aggaattagg
     3181 ggctgatata ataatcaatg ctaaagatat tgacccagta gaaaaaataa aagaacttac
     3241 tggtggaaaa ggcgttgaca tagctcttga atgtgcaggt tcaaaaataa ctcaagaaca
     3301 atgtttactt ataactaaaa agaaaagtaa gataggattt ttagggatag catattcaga
     3361 tataacttta tcagaagaag catttgaaaa tatatttaga aaagaactag aattaaaagg
     3421 tttttggaac tcttattctg caccatttcc aggacaagaa tggactaagg gaatcaatct
     3481 agttaatgaa ggaaagataa aattaaaaga aatggtatct catagattct cacttgaaga
     3541 tacttacaaa gcatttgaaa tgataagaga tagaaaagaa gaatttaaca aaatattaat
     3601 tttaccacaa ggggttgaaa aataatgaaa gcagtagtta aaactaaacc aggctatgat
     3661 aatgtagaag ttttagaagt agaagaacca aaagctacag gagacaaggt taaaataaaa
     3721 gtagaatata gtggtatatg tggttcagat atacattcat ttaaaggtga atatgcaaat
     3781 ataaaagccc cagtaacatt aggtcatgaa ttttctggaa ttgttgtaga agtaggagaa
     3841 gatgttaaag atataaaagt aggagataga gttacatcag aaactacttt tgcaacttgt
     3901 gagaaatgta tatattgtga aactaaagat tataacttat gcccaactag aaaaggtatt
     3961 ggtactcaag taaatggaag ttttgctgag tatgtactat caagagaaga aagcatacat
     4021 gtacttcctg aaaatgtatc tttattatca gcagccctta cagagccact agcttgttgt
     4081 gttcatgcag ctcttgaaaa gactactata gaaagttctg atacagtttt aataatagga
     4141 ccagggccaa ttggtttatt attagctcaa gttgttaaat ctcaaggtgc tacagttata
     4201 atgtctggtg ttacaaaaga taaggaaaga ttagaaatag ctaaaaatct tggagtagat
     4261 agaactgtaa atgtaatgga agaaagtttg gcagatgtag tgaatgaaat gactgatgga
     4321 aatggagtaa acaaaggatt tgattgttct gggtttatgc cagcagttaa cgaagcctta
     4381 agactgctta gaaaaaaagg tactttcgta caagttggaa tctttgctaa aaaattaaat
     4441 gaaatggacc aagaagcaat aatccaaaga gagttacaat atataggaag tagatctcaa
     4501 aagccaagtt cttggattac agcattagat ttaatggcaa gtgggaaagt aaatacagaa
     4561 gctttagtta ctagaactgt tggtttagac gaatttaaag aaggtattac agctttaatg
     4621 aacggggaag aaataaaagt agcagtaaaa tcttaaaaaa acatttgtat tattttaaaa
     4681 aaaattgtaa aatctactgt aggataaaaa aatgtaaggt cttaaaaaat aacaggagga
     4741 agaaagtatg agcactctta gagatcattc taataatatt agaaaaaaca taataaaaat
     4801 ggttgcagaa gctaaaagcg gacatccagg tggttcatta tcaatagctg atatgttgac
     4861 agtattatat tttgataaga tgaacgtatg tgcagaaaat ccaaaaatgg ctgatagaga
     4921 tagatttgtt ctatcaaaag gtcatgcagc accagcttta tatgctacat tagcagaaaa
     4981 aggatttttc ccagaagaag aattaataac actaagaaaa tttggttcta agttacaagg
     5041 tcacccagac atgaaaaaag tagcaggaat cgatatgtct actggttctc ttggtcaagg
     5101 gctatctgca gcaaatggta tggcacttgc aggaaaatta gataacaaag actatacagt
     5161 atatactata ttaggtgatg gagaaatcca agaaggtcaa atatgggaag ctgcaatgac
     5221 agcatcacat tacaaattag ataatttaat tgcatttgtt gacttaaatg gattacaaat
     5281 agatggttct aatgaagaag taatgaatgt aaatccagta gatgacaaat tcaaatcttt
     5341 tggatggaat gtaatagtaa taaatggaca ttcttttgaa gaaataggaa atgcagttga
     5401 tgaagctaaa aaagttacag gaaaaccaac tgtaataata gctaaaacag taaaaggtaa
     5461 aggtgtttca tttatggaaa ataatgctgc atggcatgga acagctccaa atgcagaaca
     5521 agcagagcaa gcattgaaag atttagaagg aggtctatag gaatgggaat agcaactaga
     5581 gaagcttacg gacaagtgtt aaaagaactt gcagaaaata aagatatagt agtattagat
     5641 gcagatttag gaaaagctac aaaaagtata tcatttaaag aagttgcccc agatagattt
     5701 tttgacatgg ggatagcaga aggtgacatg attggtacag cagcaggtct tgctacttgt
     5761 ggaaaaatac cttttgcgag tacttttgca atatttgcag caggaagagg ctatgagcaa
     5821 ataagaaact ctgtagcata tccaaattta aatgtaaaaa tagcagctac tcatgcaggt
     5881 gtaacagtag gtgaagatgg tggtagccat caagctatag aggatatatc tctaatgaga
     5941 ggtataccaa atatggttgt attaaatcca gcagatgctt tagaagcaag acaagctatt
     6001 cttgcatcta ttgattataa tggacctgtt tatataagat taggtagagc tgcaactcct
     6061 gatgtaaaca gtgaaaatta taaatttgaa ataggaaaag gaactgtttt aagagaaggt
     6121 tctgacataa cagtaatagc tacaggaata atggtagcaa aagccttaga agcagcagaa
     6181 gaacttgcta aagaaggtat aaatgtagaa gttgttaata tatctacaat aaaaccatta
     6241 gatgaagcat taataaaaga aactgctaag aaaactggaa aagtagttac agcagaggag
     6301 cacagtataa taggtggttt aggttcagct gtatgtgaag ctttagcaga aactaaagat
     6361 gtagtagtta gaagaattgg tgttaaagat gtatttggtc aatcaggtac accagctgat
     6421 ttattaaaac actatggttt aactacagaa gatatagtaa aaaatataaa agagttgtta
     6481 tagatataaa tatatctaaa taacaaattg atttgacata agtcaattaa agctgtctta
     6541 aaaatagaaa gaaatttcta caatgggaca gctttatttt tacaatactt tatttttatg
     6601 ataattataa aatagtctga aagtaatcaa aaaaattata aaaagaggtg ctatttaatg
     6661 aaaatatcta ttggatgtga ccatggtggt tatgaattaa aggaatatat aaaggaaaat
     6721 ttaataaata aaggtataga agttgaagat gtgggtacaa attcaaaaga aagtgtagat
     6781 tttccaatat atgcaaaaaa ggtaagtcaa aatgttcaaa gtaaaaaatc tgatttaggc
     6841 atactttgtt gtggaacagg aataggcatg tctattgcag caaataagtt taaaggaatc
     6901 agagcagctg ttgtttctga ctgtttttca gctaaggcaa ctagagaaca caataataca
     6961 aatgtacttt gtttaggaga aagagttatt gggaaaggat tagcaatgag aattgttgaa
     7021 gaatggttaa attcaagtta taatggagaa agacatgcaa gaagattaca aatgattgat
     7081 gatatagaaa aagaaaattt ataaatatat ataatatata atttataaca agggggagcg
     7141 ttatgtctat tatttgtgct tcaataatgt gtgcagacca aatgaagtta aaagaggaat
     7201 tagaagctct tgaagaagca ggtgtaaaat tacttcattg tgatgttatg gatggaatat
     7261 ttgttaaaaa cttggctatg ggaccagagt tattaaaatc aataagtgaa aatacaacaa
     7321 tacctttaga catacactta gccacagaaa ctccagataa atatattgat atgatgtctt
     7381 atataaagcc aaaatatatt tcttttcatg tagaatcaag tacaaatgta aaagctgata
     7441 tacaaaagtt aagaaattat ggtatagggc cagtgttagc aataagccct caaacaagtg
     7501 tggataaaat agaagaatat ataagtttag ttgagggtat cttagtaatg acagtaaatc
     7561 cagggtttgc aggtcaaaag tttaatcttt ctgtattgga taagttggat aaacttacag
     7621 agatactaaa agattatgat aatcctccat ttatagaggt tgatggaaat ataaataagg
     7681 atactattaa acttatgaat ggtaaaaaag tagatatata tgttgttggt acttcagctt
     7741 tatttaatga taagcctcca atttcatata aagataaaat agaagaacta aaagaaagta
     7801 taaagtaacc atgtaataaa attataatat gtttctgttt aatagtaaaa atttaggtgg
     7861 aaacaagaaa ttttataata tttaataaaa attttggttg agtaaggaga attttttatg
     7921 ggagacctat ataaactaca aaatggtact gatattagag gaatagcgta tgaaaatgat
     7981 tctaaagaag ttaacctaac tgtagaagaa gtaaaaaaaa tagctaaagc gtttcatatt
     8041 tggctaaaag aaaagactaa gaaagataaa gtaactgttg ccataggaac tgattctagg
     8101 ataacaggaa gccaatttag aagtactgtt attgaaactt taactaatga tgattgtaat
     8161 gtaattgatt gtgagattgc tacaactcca gctatgttta tgacaactat tatggatgga
     8221 tacaattctg atggggcgat tatgattaca gcaagtcact taccttatta ctataatggt
     8281 ttaaagtttt tcacagaaag tggaggttta gaaaaaacta atataaaaga gatgttggat
     8341 atagctgtta aaaatgatag tgtagactat gaggaagtaa ataaaaaagg tgaagttata
     8401 gtaaaaaatt taatagaaga ttattctaat ttattgatag ataaaattag aaaaggtgta
     8461 aattcaagta aaaattatga aaagcctttt tctgggctta agatattggt tgatgcagga
     8521 aatggagctg gtggattctt tgctgaaaaa gttttacaca tactgggagc agatacgaca
     8581 ggcagtcagt ttttgaaccc agatggtatg ttcccaaatc atatacctaa tcccgaaaac
     8641 aaagaagcaa tggaaagcat atgtaaagct gtgttggata ataatagcaa cctaggaata
     8701 atctttgata cagatgtaga cagagctgct attgtaggta aaaatggcaa accaataaat
     8761 aaaaatgctc ttatagctgt tatatcatca atagtattag aagaacatcc aaaaacagct
     8821 atagtaacag attcgattac ttctgaagga cttgctaaat ttataaatga attacaagga
     8881 agacatcata gatttaaaag aggatataaa aatgtaataa atgaagctat aagattgaat
     8941 agtgaaggtg aagagtgcca tttagctata gaaacgtctg gacatgctgc tttaaaagaa
     9001 aattatttct tagatgatgg ggcatattta atagctaaaa ttttaataaa agtagcaaag
     9061 ctatccttgg aaggaaagac tatagaagaa ttgatagaaa accttgaaga agctcaagaa
     9121 gagaaagaag ttagaatagg cattaacaaa gaagatttta gaccatatgc agaggatata
     9181 ttaaaagatt tagattctca tgttaagagt attgatggat ggtctgtagc accaaataat
     9241 tttgaaggaa taagagttaa ttgtgatgaa tcaaatggag atggatggtt tttacttaga
     9301 atatcactac atgaaccatt actagcttta aatattgaat caaataaaat tggtggagca
     9361 gaacaaattt attctaagtt aaaacttttc ttagaaaaat atgatttaaa aggtctttag
     9421 ggtaaataga aagaatatat ttatattaaa gaacaaatgt aaatttgtac ctaagattac
     9481 aaatattaca aaattgtaat cttaggtaac agagatgtaa ctgattatgg aaacactatg
     9541 tgatattata gatacagaga gatgaaatat gtgatgaatt atagagaaat ataatttaaa
     9601 atgtattaat atcacaccaa aaaggtataa tcaaaaaata tgtaaatata aaccccaaca
     9661 tttagaaaaa gacctctaag gaggtttttt tcttgtttaa ataacttaaa atatattttt
     9721 atattaatat atcaattaac tcttctaaaa gtaagcatta ccttaaataa atcagcatct
     9781 ataattattt ccatgtgtgc attttctaat tcaattatat tttgagcaat tgataaacca
     9841 agaccagagc catctgtttt tctagatata tcaccccttc taaatctttc tattaactct
     9901 tctggcttta gatttagttt atcatttgaa atatttttaa acgttaatat tatattttct
     9961 tcctttataa acatatctat atagacccta gtgttaggca tagagtattt tagtatattg
    10021 caaattagat tctcaaaaac tctccaagtt tttttaccat ctatcatggt aaaaacagca
    10081 tattcaggtg tttctattat aaaatctaaa ctgctatttt gaattttgtc ttcaaactct
    10141 acaatacttt gaattaaaag ttctcttata tcggtttttg ttatattaaa ttctacatct
    10201 ccactactta gcttagatgc ttcaaatatg tcatctatta ataattttaa tctttgtgat
    10261 tttttatcta aaatatttat atagtcattt atagtctctc tgtcaaagtc attatttttt
    10321 aacatatcaa catagcttaa tatagatgta agtggagttt ttaagtcatg agatacgttt
    10381 gtgataagct ctgccttcat cctttcattt tttatattag catctacagc agtttcaaga
    10441 ccacctgtta tattatttat acttttggct aaagtagata tttcatcatc tcctataact
    10501 tctaatttat actttaaatt gccattttct atatttttta tattttttga tatgttattt
    10561 atgtaattaa acttaacaga taaaaagttg tatatataaa aatgaaaaac tgtgtttaca
    10621 agtgtaaata aaattactat agctattgcc tcagaataat atatatacat aaagaatatc
    10681 aaaacaagat attggataat agcttgtaca actatagata ttaaaaatac tatagctaat
    10741 tttttattta tagttttttt attatacata ctaagtaagt ttgaaaagaa tacatatgtt
    10801 atagacatat cttttagatt atagttatta tatttaatat tttcatatat tatatagact
    10861 atagatataa ttataaaatt gtagattatt aaattagtgt tgtaaaacat tttattactt
    10921 gtaaatgcat gaaaccttaa ggttaatagt atcaaagtta atataagtgt aagttctatt
    10981 tttaccttag aaaaaaggct tatgacattc tctaagaatt tgactttagt ctgtttattt
    11041 tttttataca atgtaaaaca tagtatagtc aataaaaaac ttgataatat aatacctatg
    11101 cagacaaact ctaattctac ttttgacaaa tgtacaggag taccgaaaaa ataaaaataa
    11161 gggagagcaa actctttata ataacaatta atattataag aaaagttatt attcaaatca
    11221 taaactgctt caaataatat agaaactaat agaaacagta ataataataa aataaatttt
    11281 gctttagaat cattaataat taagtttttc aattttgtaa ccaatgcccc acaccacctt
    11341 taaatatttt ggttcttttg gattaatttc aattttttct ctaattcttc taatatgaac
    11401 agcaatagtt ctctcacaac tataagcatc ttcattccag acattttcat aaatttcacg
    11461 ggaagagaaa attttacctt ttcgtgacat aagaagtgta agtattttat attcaagtgg
    11521 tgtcatttta atttcctcac catctaaaga aactattttt gtatctgtat ttagctctag
    11581 gccaccactt tttagaatat aattagtatc atttgagtta tttgtattac taaaatcacc
    11641 taaatttata taccttctaa gttgagattt tactcgagca gtaagttcta gtggattaaa
    11701 tggtttagtt atatagtcat cagcacccat attaagacct ataattttgt ccatatcttc
    11761 tgattttgca gataagagaa ttattggtat attccttttt tctcttattt taagtgtagt
    11821 tttaagccca tctaaattgg gcatcattac atccattatt attaacttaa tatcattttc
    11881 gattagtatg tccaatgctt caagtccatc atatgccttt aaaacattaa agttatcagg
    11941 tctcaaatag aactctatag catcaactat ctctctatca tcatctacaa ccaatatatt
    12001 ttgattcaat ttattaaccc ccttgaaatt ttatttttaa ccaattttta ttttatacca
    12061 aaaagaggta ttagaaaaca gtaagcccgt tcattatgaa ataaaaacta gtacttttaa
    12121 tatattgtca atgcatatag taaatttaaa agtatagacg cccatattaa tctgaaggta
    12181 aaagtttatg aaaatatcta gggtaatcta tcacactcct tatgttattt tgggtttatt
    12241 aattttttag gtttaaacat atattaacaa atataaataa caaatgagtt tcataattat
    12301 taagaattta taacaaatat ttattttaat gtagtttaa
//
LOCUS       DAELXX010000110        12309 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.110, whole genome shotgun sequence.
ACCESSION   DAELXX010000110 DAELXX010000000
VERSION     DAELXX010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12309
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.110"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            480..1679
                     /gene="hadA"
                     /locus_tag="KPH29_002994"
     CDS             480..1679
                     /gene="hadA"
                     /locus_tag="KPH29_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HBG1468573.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            1718..2518
                     /gene="hadI"
                     /locus_tag="KPH29_002995"
     CDS             1718..2518
                     /gene="hadI"
                     /locus_tag="KPH29_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HBG1468574.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            2533..3759
                     /gene="hadB"
                     /locus_tag="KPH29_002996"
     CDS             2533..3759
                     /gene="hadB"
                     /locus_tag="KPH29_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HBG1468575.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            3759..4886
                     /gene="hadC"
                     /locus_tag="KPH29_002997"
     CDS             3759..4886
                     /gene="hadC"
                     /locus_tag="KPH29_002997"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HBG1468576.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKNETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            4934..6067
                     /locus_tag="KPH29_002998"
     CDS             4934..6067
                     /locus_tag="KPH29_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBG1468577.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            6100..6885
                     /locus_tag="KPH29_002999"
     CDS             6100..6885
                     /locus_tag="KPH29_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG1468578.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            6904..7941
                     /locus_tag="KPH29_003000"
     CDS             6904..7941
                     /locus_tag="KPH29_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBG1468579.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            8424..10055
                     /locus_tag="KPH29_003001"
     CDS             8424..10055
                     /locus_tag="KPH29_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG1468580.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(10190..11116)
                     /gene="fba"
                     /locus_tag="KPH29_003002"
     CDS             complement(10190..11116)
                     /gene="fba"
                     /locus_tag="KPH29_003002"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HBG1468581.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            11446..11775
                     /locus_tag="KPH29_003003"
     CDS             11446..11775
                     /locus_tag="KPH29_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HBG1468582.1"
                     /translation="MPQIKIRGINENDICKISKKMINDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESMDLAFTVFEKEKY
                     YENGEHF"
     gene            11775..11978
                     /locus_tag="KPH29_003004"
                     /pseudo
     CDS             11775..11978
                     /locus_tag="KPH29_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
BASE COUNT         4918 a         1491 c         2314 g         3586 t
ORIGIN      
        1 ttgttatttt ttagattaaa tattattaaa aaattatcta agtttgaaat tagttaacta
       61 acttgctgat taattacaat gataacatgt gtttttgagt ttagcaatga aaactttttt
      121 tgaaatgtca agctttttca taaaaaaata tataaaaaaa acttttataa gcatagaaag
      181 aatagcaaaa tgcaatggat agtggcaaga tgccacgata tgaaaaagat tattcaattg
      241 catattgtta aaaaacattc aaatcaagcg atattttgat taaatataaa gttggcacga
      301 taaatgctta acaatatagg tgtaagacaa taaaacaata aaaaataatt ttaataattt
      361 ttaagttatt ttagataaga ttaggaactt tgacatcaga cagttaatat cagaaaaaag
      421 tattttatga gtgatttaat cgaaatttta taattgatac tattaaggag gaaaacgtta
      481 tgcttttaga aggagttaaa gtagtagaac tttcaagttt catcgcagca ccatgttgtg
      541 caaaaatgtt aggtgactgg ggtgcagagg ttattaagat tgaacctata gaaggtgatg
      601 gaataagagt tatgggtgga acatttaaat ctccagcatc agatgatgaa aaccctatgt
      661 ttgaattaga aaatggaaat aaaaagggtg taagtattaa tgtaaaatca aaagaaggag
      721 tagaaatatt acataaatta ttatcagaag cagacatatt tgtaactaat gttagagttc
      781 aagcattaga aaaaatgggt atagcttatg accaaataaa agataagtat ccaggattaa
      841 tattctctca aatattagga tatggtgaaa aaggaccttt aaaagataaa ccaggatttg
      901 actatactgc atacttcgca agaggaggag ttagccaatc tgttatggaa aaaggaacat
      961 ctccagcaaa tacagcagca ggatttggtg accactatgc aggtctagca ctagcagcag
     1021 gaagtttagc agcattacat aaaaaagctc aaactggtaa aggtgagaga gtaacagtaa
     1081 gtcttttcca tacagctata tatggaatgg gaacaatgat aacaacagca caatacggaa
     1141 atgaaatgcc tttatcaaga gaaaatccaa acagcccatt aatgactaca tataaatgta
     1201 aagatggaag atggattcaa ttagctttaa tacaatacaa caagtggtta ggcaaattct
     1261 gtaaggttat aaatagagaa tatatattag aagatgatag atataataac atagattcaa
     1321 tggttaatca tgttgaagat ttagttaaga tagttggaga agctatgtta gaaaaaacat
     1381 tagacgagtg gtcagcttta ttagaagaag cagacttacc atttgaaaaa attcaaagct
     1441 gtgaagattt attagatgac gaacaagctt gggcaaatga cttcttattt aagaaaacat
     1501 acgatagcgg aaatacaggt gtcttagtta atactccagt tatgtttaga aatgaaggaa
     1561 ttaaagaata tacaccagca ccaaaagtag gtcaacatac tgtagaagta ttaaaatctt
     1621 taggctacga tgaagagaaa ataaataact ttaaagatag taaagttgta agatattaat
     1681 tagtaattct agatttttta aaaggagaat gctaaagatg tacacaatgg gattagatat
     1741 aggttcaact gcatcaaagg gagtaatctt aaagaatggg gaagatattg tagcttctga
     1801 aacaatatcc tctggtactg ggactactgg accatcaaga gttttagaaa aattatatgg
     1861 caagacaggt cttgcaagag aagatattaa aaaagttgta gttacaggat atggaagaat
     1921 gaactattca gatgctgata agcaaataag tgaattaagc tgtcatgcta gaggggtaaa
     1981 tttcataatt ccagagacaa gaaccattat tgacataggt ggtcaagatg caaaggtatt
     2041 aaaattagat aataatggaa gactattaaa ctttcttatg aatgacaaat gtgctgcagg
     2101 tacaggaaga tttttagatg taatggcaaa aataatagag gttgatgtat ctgaactcgg
     2161 aagtatatct atgaattctc aaaatgaagt atcaataagc agtacatgta cagtatttgc
     2221 agagtctgag gttatatcac atttatctga aaatgcaaaa attgaagata tagtggcagg
     2281 tattcatact tcagtagcaa agagagtttc tagcctagta aaaagaatag gagtacaaag
     2341 aaatgtagtt atggttggtg gagttgctag aaatagtggt attgtaagag ctatggcaag
     2401 agaaatcaac acagaaatta ttgtacctga tatacctcaa ttaactggtg ctttaggagc
     2461 agcgttatat gcttttgatg aagcaaaaga atcacaaaaa gaagtgaaaa atatataaaa
     2521 agagggatga aaatgtctga aaaaaaagaa gctagagtag taattaatga tttattagct
     2581 gaacaatatg caaatgcatt taaagctaaa gaagaaggaa gacctgtagg ttggtcaaca
     2641 tcagtatttc ctcaagagtt agcagaagta tttgacttaa acgtattata tccagaaaac
     2701 caagcagctg gagtagcagc taaaaaaggt tctttagaat tatgtgaaat agctgaatct
     2761 aaaggatatt ctattgacct atgtgcatat gcaagaacaa attttggtct tttagaaaat
     2821 ggtggatgtg aagctttgga tatgccagct ccagatttcc tactttgctg taacaatata
     2881 tgtaatcaag ttataaaatg gtatgaaaat atttcaagag aattagatat acctttaata
     2941 atgattgata caactttcaa taatgaagac gaagttactc aatcaagaat agattatatt
     3001 aaagctcaat ttgaagaagc tataaaacaa ctagaaatta tatcaggaaa gaaatttgac
     3061 cctaagaagt ttgaagaagt aatgaaaata tcagctgaaa acggaagact atggaagtat
     3121 tctatgagtt taccagcaga ttcttctcct tctccaatga atggatttga cttatttact
     3181 tacatggctg taatagtttg tgctagaggt aaaaaagaaa ctacagaagc atttaagtta
     3241 cttatagaag aattagaaga caacatgaaa actggtaaat cttctttcag aggggaagaa
     3301 aaatacagaa taatgatgga aggtatacct tgttggccat atataggata caagatgaaa
     3361 acattagcta aatttggagt taacatgaca ggtagtgttt acccacatgc ttgggcatta
     3421 caatatgaag ttaatgattt agatggaatg gcagtagcat atagtactat gtttaacaat
     3481 gtaaacctag accgtatgac aaaatataga gttgattctt tagtagaggg taaatgtgat
     3541 ggagcattct atcatatgaa cagaagctgt aaacttatga gtttaataca atatgaaatg
     3601 caaagaagag cagctgaaga aactggatta ccatatgctg gatttgatgg tgaccaagca
     3661 gaccctagag ctttcactaa tgctcaattt gaaacaagaa ttcaaggttt agttgaagta
     3721 atggaagaaa gaaaaaaact taatagaggt gagatataat ggaagctatt ttatctaaaa
     3781 tgaaagaagt agttgaaaat ccaaatgcgg ctgtaaaaaa atataaaaat gaaactggta
     3841 aaaaagctat aggttgtttc ccagtttatt gcccagaaga aattatacat gcagctggaa
     3901 tgcttccagt tggtatatgg ggaggacaaa cagaattaga tttagctaaa caatatttcc
     3961 ctgcatttgc atgttcaata atgcaatcat gtttagaata tggattaaaa ggtgcttatg
     4021 atgaattatc tggagttatt ataccaggta tgtgtgatac actaatttgt ttaggacaaa
     4081 actggaaatc agcagtacct catataaaat atatatcatt agtacaccca caaaatagaa
     4141 aacttgaagc tggtgtaaaa tacttaatca gtgagtacaa aggtgtaaaa agagaacttg
     4201 aagaaatttg tggatatgaa atagaagaag caaaaattca tgaaagtata gaagtttaca
     4261 atgaacatag aaaaactatg agagactttg ttgaagtagc ttataaacat tctaatacta
     4321 taaaaccatc aataagaagc ttagtaatta aaagtgggtt ctttatgaga aaagaagaac
     4381 atactgagct agtgaaagat ttaatagcaa aattaaatgc tatgccagaa gaagtctgtt
     4441 ctggaaaaaa agttttatta acaggtatat tagctgattc taaagatata ttagacattt
     4501 tagaagacaa caatatatca gttgtagctg acgacttagc acaagaaaca agacaattca
     4561 gaacagatgt accagcaggt gatgatgcgt tagagagatt agcaagacaa tggtcaaaca
     4621 tagaaggatg ttcattagct tatgacccta agaaaaaacg tgggtcactt atagtagatg
     4681 aagttaaaaa gaaagatata gatggtgtta tcttctgtat gatgaaattc tgtgacccag
     4741 aagaatacga ttatccttta gttagaaaag atatagaaga tagtggaata cctactttat
     4801 atgttgaaat cgaccaacaa actcagaata atgaacaagc cagaactcgt attcaaactt
     4861 ttgctgagat gatgagttta gcgtaagttc attaaaataa atttactata aaatatgaaa
     4921 attggaggaa gctatgttat ataacaaaga acaagaactt ttaagaaaag cagtaagaga
     4981 ttttgttagt aaagaattag atactttacc agcagaaatg gataaaacag gtgtaatgcc
     5041 taaggaatta atcaaaaaat tagctgatgc aaaatttata agcagtaata tcccagaaga
     5101 atatggtggt gggggagcag gatatgtttc ttacgctata gtaatggaag aaatagctag
     5161 aagatgtgct tcaacagcta cttttgttac agctggttct tctcttgctt cattaccaat
     5221 actttacaat ggtactgaag agcaaaaaca aaaatactta aaaggtatag caacaggtga
     5281 attaatagga gcatttgggt taactgagcc aggagctggt tctgatgcag gtggacaaca
     5341 aacaacagca gaattagttg gagaccacta tatattaaat ggtagaaaaa ctttcataac
     5401 taatggacca ttctgtgatg tagctatagt aatagcagtt acagatagaa gtaaaggact
     5461 tagaggaaca tcagcgttta tagtagaaag taaatgggat ggtttctcaa caggagctca
     5521 tgaagataag atgggtataa gaggaactga aacttctgac ttaatatttg aaaacgttaa
     5581 agttccaaaa gaaaacttac ttggaaaaga aggtcaagga tttaagatag caatgggtac
     5641 tctagaagtt ggtagaatag gtgttgctgc cttagctcta ggaatagctc aaggtgcttt
     5701 agatgaagct gttaaatata caaaacaaag agttcaattt ggtaagccta tagctaaatt
     5761 ccaaaatact caatttacta tagctgacat ggaaacaaaa gtttgtgcag ctagaggatt
     5821 agtttatgat gcagcacaaa agagagatgc aggaatgaga gttgctcaag aatctgctat
     5881 ggctaaatac tatgcatcag aaattgcaaa tgaagttgct tataaagcat tacaacttca
     5941 cggtggatat ggatttataa aagattatga aatcgaaaga atgtacagag atgctagaat
     6001 cgtatcaata tacgaaggaa catcagaagt tcaaaaaatg gtaatttcat caaacgtatt
     6061 aaaataataa ataatctaat gaaattggag gtatagactt tgaaaatatt agtttgcgta
     6121 aaacaagttc cagatactaa cgaagtaaga ataaataaag aaacaggtac tcttataaga
     6181 gatggagttc caagtatatt aaacccagat gatgcaaatg cattagagga agctttaaaa
     6241 attaaagatg agcatgatga tgtaactata acagttataa caatgggacc tcctcaagca
     6301 gactttatgc taagagagtg tttagctatg ggtgctgatg atgcaatatt attaagtgat
     6361 agagcatttg gaggagctga tacttgggct acatcaaata ctattgcagc gggaatatct
     6421 aaagtaggtg actatgacat aatatttgca ggaagacaag caatagatgg agatactgca
     6481 caagttggac cacaaattgc agagaaatta gacattcctc aagttacata tgtacaagat
     6541 ttcaaaatag aaggaaaaga tataatagtt caaagacaat tagaagatgg atacgaatta
     6601 ataaaagtaa gtacacctgt acttttaaca gcagtaaaag agttaaatac acctagatac
     6661 atgtctgttg ataaaatagt aaaagcatat aaacatgatg taaaagtttg gacaattgat
     6721 gatttagatg taaataaaga agaagttggt ttaaaagcat caccaactaa agtatttaga
     6781 tcatttactc ctgaaccaag aggaaaagga gaaatcttag aaggtaaagc tgatgaaata
     6841 gctagtaaaa taataatcgg tctaaaacaa aagcacatta tataagttgg aggagtacaa
     6901 actatgaatg atataaaaga tttaagttct tataaaaacg tatggatatt tgcagaacaa
     6961 agagaaggaa aaatagctcc agtagttata gaattattag gagaaggaag aaaattagct
     7021 aaagaagtag atgcagaact ttgtgcaata ttattaggaa aagatgttga tggattagct
     7081 aaagaattaa tcacttttgg agctgacaaa gtttatgttg cagatgatgc tcttttagaa
     7141 aaatatacaa ctgatgcata tacaaaagta ataaaagatg caatagatga aataaaacca
     7201 gaaataatgc ttttcggagc aactcatata ggtagagact tagcacctag aatagcttca
     7261 agagttggaa ctggattaac agctgactgt actaagttag aaatagaccc agaagataag
     7321 aaaataaaac aaactcgtcc agcatttggt ggaaacataa tggctacaat catttgtcca
     7381 aaccatagac ctcaaatgtc tacagttaga cctggtgtta tggataaagc tgaaaaagac
     7441 gaaacaagaa ctggtgaagt tatagcatta gactacaaaa taactcaaga tgatataaga
     7501 actactgttt tagaaacagt taaaactaag aaagatttag tatctcttac agatgcaaat
     7561 gttatagtat caggtggttt aggattaggt ggaccagaag gatttgaaat gcttaagaaa
     7621 ttagctgaca aattaggtgg agtagttggt tcttctcgtg cggctgttga tgctggatgg
     7681 atagaccatt ctcaccaagt aggtcaaaca ggaactactg ttaaaccaaa cctatatata
     7741 gcttgtggta tatcaggagc aatacaacat ttagcaggta tgcaatcatc agatttcata
     7801 attgctataa acaaaaaccc agcagctcca attttagaaa tcgctgacta tggagtagtt
     7861 ggagacttac atgaaatagt tccaatgctt atagaaaaat tagatagcgt tgatgattta
     7921 ttagaagcta taaaagctta atagatacaa actatataaa tattactgat tgaataagtc
     7981 ttattagtta gtaacataat tattaaccct ttgataagtt attgctagtg tgtatttgtc
     8041 agttattaaa aagtctgttt gaataaaatt ttataataca agataatctg gataatcctt
     8101 atatgtatat tgcttacaaa ttgtgagtta tattctgtaa ggattatctt tgttttttta
     8161 aataaatctt aaatttaatt aaattgtatt aaatagacaa ataattacta aatatgtgat
     8221 aaaatacaat taaaagcaaa atgaaatttt taacaaacta taatcattaa aattatatct
     8281 tagttacgtt ttaatgaaat aaactacttg aaaagacgaa tattataagg agttgattat
     8341 atgtgctgat ggtatttatt gtaagcaaac aataaatttt gtatgtagtt caataaatta
     8401 cttaatgaaa ggtgcgaata ttaatggata ataatttaat agggcttata gaacatgtag
     8461 aaaatccagc cattttatgt aaagaatcag gcgaaataat atactgtaat catctaatag
     8521 atagtatttt tagttttcta gatataaaaa agcctagaaa tataaatgaa ttagattcaa
     8581 attttgacaa aacagaaata ctgacagata gtaaaaagaa aatagctttc agagagttaa
     8641 ggatgactgc acatatatac aatatgaaag ataataataa tgaaaataat atagtttatt
     8701 tatttgagaa gtctctaata tctgataaag taattgaaga tataatagag catatagatg
     8761 aagttgttgt tgtgtttaac aaagatgggg taattgaaaa gatgaacact gtcagtgatg
     8821 agatattacc ttttaaaaga actgaggtac ttggtagaaa tataacagac cttgtgagac
     8881 aaggattagt tgaagaacca ataatattaa acatgcttaa agtgaagaaa aagatttata
     8941 gaaatatcgt ttacccagat gggaagctta ttgcatatac ggcagttcca agatgggatt
     9001 ctaagggaaa gcttacagga ggagtactaa cagggagaga tatttcaagg gttattaagc
     9061 ttgaatctca gataaaatat agtgatatat cagaagacac agagtatata agtcaaagta
     9121 agattatgga caatataaaa aaagttgtta agagagctgc agcctcagat tcttctatat
     9181 ttataaatgg agaatcaggt gtaggaaaag aaataatagc aagaacaata tataagtata
     9241 gttcaaggag agacaaacct tttatagcta taaactgtgg agctatacca aatgaattat
     9301 tagagtcaga gttttttgga tatgaagaag gttcttttac tggagctaag aaaaaaggta
     9361 aaaagggact ttttgaagag gcgaatggtg gaactatttt cttggatgag ataggtgagt
     9421 taccaatgca gatgcaaaaa aaattactta gggttataca agaaaatact attactagaa
     9481 taggtggtag taagcctata aaaatagatg ttagatatat aagtgctact aatatttctc
     9541 atgaagacct tagaaataac cttaaattta gacaagattt gtactataga ttaagtgtta
     9601 ttccagtaaa aatacctcct cttagagaaa gaaaagagga tatagtacca ttagtaaatt
     9661 atttcttaaa actttacaat gaaaagtaca atagagaagt agaagtttca cctaaagtta
     9721 ttgagttatt agaagaatat tcttggccag gaaacataag agaattaaaa aacatcattg
     9781 aaagatttgt agtattatca gctaaaaatg taattggaga agatgaattt aacatgctga
     9841 taaatttaga tatgattgat aatgaaacag atgatttatc accaatagtg gtaaatggaa
     9901 ttatgaatct taatgatgca tataaaatag tggaccaaat aatgataagt aaggctataa
     9961 ataagtatgg ttcaataaca aaggctgctg aagttatagg tatatatcct tctaccatcc
    10021 atagaaagat aaagagtgga catattcacg tttagctgtc agtttaaaaa gacaatataa
    10081 ttaaaatatt ataagataca taattgatac aacttaaaag agatggctta aaaataaatt
    10141 taattacttt aagtcatctc ttttctttta taaatattta aaagttatat tagtttatag
    10201 agttagcaga acctaaaaca tcatctatct tgctttcaac tacagcttgt atagcatctc
    10261 taccagcacc aatgtatttt cttgggtcaa attcttttgg attttcagct aagaattttc
    10321 tgatagcagc agtcatagct agtcttaagt cagtatccat gtttatttta caaacagcca
    10381 tagaagatgc ttttcttaac atatctacag gtacaccttt agcaccagct atatttccac
    10441 caaattcatt acatgtagca acagcgtttt ggtcaacagc agatgcacca tgaagaacta
    10501 ttgggaaacc tggtaatttg ctttggattt cttctagtat atcaaatctt aatttagctt
    10561 ctcctttgaa tttgaaagca ccatgagaag ttccaatagc tattgctaat gaatcaacac
    10621 ctgttctttc aacgaactca actgcctcag ctggttgagt atacttgtga acatcagaag
    10681 taacatcatc ttctgttcca gctagaacac caagttctgc ttcaacaaca acacctttag
    10741 agtgtgcata ttcaacagct tctttagtta ttctaacatt ttcttcaaag tcaaaatgag
    10801 aaccatctat cataactgaa gagaaaccag catcaataca agttttaata gcatccatat
    10861 ttggaccatg gtctaagtga agagctacat ctacacctat ttcatcagaa gcagccttaa
    10921 ccatttcaac taatgtatga ggtccagcat atttaacagc tgacatagaa gcttgtatca
    10981 taacataaga atttttagct ttagcagctt ttaaaacacc ttgtaattgt tctaagtcac
    11041 ttatgttaaa tgcacctata gcaaatccac cttcgtaagc ttttttaaac atttcttttg
    11101 tagtaattaa tgccatttta aactcctcct attaactttt aaaatttacc catcacttat
    11161 attataacgt taaatcaaaa aaagtatata gtaaattgta ataactataa attacaagta
    11221 taatgttaaa tagaagacaa taggattaaa taaaataata aagaatataa ataatttagg
    11281 aaaattagct atataatgct aactataagg gtaggaacta ccttattagt ttggagatac
    11341 tgataacaat agttgtcttg accaagaagt taccacttca aacgttatcg aagggaatta
    11401 agtggatgat aattcactaa aaatttaaaa taaaggagat aaactatgcc acagataaaa
    11461 ataagaggaa ttaatgaaaa tgatatatgt aaaataagta aaaaaatgat aaatgattta
    11521 gttgaagctg taaaatgtcc aagagactac tttgaaatag aatgtataaa gtcagttgca
    11581 ataagagatg gtaaaatagc agatgtatat ccttttgttg aagtagcttg gtttgataga
    11641 ggacaagaag tacaagatat agtagcaaga ataataactg atagtataag aaataattta
    11701 gatgtagaaa gtatggattt agcatttaca gtatttgaaa aagaaaaata ttatgagaat
    11761 ggagagcatt tttaatggct aaaaagcaac gtatagataa aattctttca aatttaggtt
    11821 atggaagtag aagtgaaata aagaagtatt gtaaacaagg gtctgtagta gtaaatggaa
    11881 gtgaagtttc aaatccagga actcaagtag acactgaaaa tgatgagata ttatttaatg
    11941 gagaagaagt tatatataga gaatatatat atttgattaa aaaatagcta cgtacactcg
    12001 tgactttagt catgagttag tagctaaaat agtcagcata tagggaaact tatatgtaga
    12061 gataggatag agcctatcca atactacttg aattgctgga aacccctaaa gctaaccaaa
    12121 ctaaaacata aggatgaaat aaacctaagt gtgaaagtta cgaaagtaga aaaaattggt
    12181 tagatggtat aaggttaaac cctaagtact ttaaaatggg caatcagcag gtaagctccg
    12241 aataggagaa acttcaacga ctattcctct tgagggaagt acactacaag ctatcggtag
    12301 tggaagtgg
//
LOCUS       DAELXX010000111        12013 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.112, whole genome shotgun sequence.
ACCESSION   DAELXX010000111 DAELXX010000000
VERSION     DAELXX010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12013
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.112"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(434..2014)
                     /locus_tag="KPH29_003005"
     CDS             complement(434..2014)
                     /locus_tag="KPH29_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HBG1468583.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGDENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSTLIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     NKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTVN
                     ATDLISNISLTMKELLDI"
     gene            2812..3713
                     /locus_tag="KPH29_003006"
                     /pseudo
     CDS             2812..3713
                     /locus_tag="KPH29_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            3700..4455
                     /locus_tag="KPH29_003007"
     CDS             3700..4455
                     /locus_tag="KPH29_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG1468584.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQVLLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQSLSLIM
                     SCLTSPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITTLMVTHNLKHALHYGDRL
                     IMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            4586..5608
                     /locus_tag="KPH29_003008"
     CDS             4586..5608
                     /locus_tag="KPH29_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG1468585.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKGKEV
                     KKIGITQLVEHPALDATRIGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
     gene            complement(5772..6329)
                     /locus_tag="KPH29_003009"
     CDS             complement(5772..6329)
                     /locus_tag="KPH29_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBG1468586.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPVKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(6335..7411)
                     /locus_tag="KPH29_003010"
     CDS             complement(6335..7411)
                     /locus_tag="KPH29_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HBG1468587.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            complement(7412..8095)
                     /gene="modB"
                     /locus_tag="KPH29_003011"
     CDS             complement(7412..8095)
                     /gene="modB"
                     /locus_tag="KPH29_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBG1468588.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(8244..9056)
                     /gene="modA"
                     /locus_tag="KPH29_003012"
     CDS             complement(8244..9056)
                     /gene="modA"
                     /locus_tag="KPH29_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG1468589.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKTDDGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMKDLTTDKVKKIAVGEVESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            9249..10178
                     /locus_tag="KPH29_003013"
     CDS             9249..10178
                     /locus_tag="KPH29_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1468590.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
     gene            complement(10339..10788)
                     /locus_tag="KPH29_003014"
     CDS             complement(10339..10788)
                     /locus_tag="KPH29_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468591.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPHIYKKARVLMFKTYHMLIYIKEKYYYVNIY"
     gene            complement(10991..11971)
                     /locus_tag="KPH29_003015"
     CDS             complement(10991..11971)
                     /locus_tag="KPH29_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG1468592.1"
                     /translation="MKNSFKNCISLRNKFIIISIVFLLILLIVSYIFGKLVYNSSVGL
                     EQLIKNEDIVKIFSERKDKPLEKLKNYKTSELMIQSSNNYKLESLFITSNIKTRDTMI
                     LVHGIGSSYYEMLKVAYRYLDKGYNVLVYNQRNTGNSGGDNYTFGLYERYDLDSLVKF
                     VKNKFPEGRLGVHGFSMGAGTAAMHSEINSKDDKVDFYILDSPYSEMKDAIRMGVLEK
                     RIPDILINYVVICGDLYNKFKSGFWYSDVKPYESVEKSNVPILFIHGTKDTVCNYQNS
                     KKMYDLVKHDKKDLWLIEGIGHVDGFEHDSTVYFNKIFKFIDSNVLSDKS"
BASE COUNT         4080 a         1997 c         1418 g         4518 t
ORIGIN      
        1 ttcgctacta cctatgcagt tctcttatga acttcttaca ctttcatgta agcacagact
       61 atatcttatc cttcggcatt atccgctaag gtctatccac ttccactacc gataacttgt
      121 agtgtacttc cctcaagagg aatagtcgtt gaagtttctc ctattcggag cttacctgct
      181 gattgcccat tttaaagtac ttaggattta accttatacc atctaaccaa ttttttctac
      241 tttcgtaact ttcacactta ggtttatttc atccttatgt tttagtttag ttagctttag
      301 gggattttag caattcaagt agtattggat aggttctatc ctatctctac atataggttt
      361 ccctatatgc tgactatttt agctactaac tcatgactaa agtcacaagt gtgcgtagct
      421 attttttaat caattatata tccaacaact ctttcattgt taatgaaatg ttacttatta
      481 aatccgtagc atttaccgta tacattgctt tgctcaattt atctcctata tagccatgta
      541 tatatgctcc actaactgta gcttccaata tatccatacc ttgacctata aatgaagtaa
      601 tcattccaag caaacaatca cccattccac cagttgccat acagctattt cctgtaggat
      661 ttatatatgt ttttttacca tctgttatga cagtttgata accttttaaa agcaccacca
      721 gattatattt ttgagcaaat tcttttgcaa catctactct atgttctctt atatacccaa
      781 ttgaatctcc tgtcaacctg gccatttctc ccaaatgagg tgtaattaca aatctatttt
      841 tccattctaa aaacttataa catctgtcct tcataacgtt caatccatct gcatctaata
      901 ctattgggca attagaattt tctactatct taagtaattt atcaaatgtt tgactattat
      961 ctcccatacc tggaccaaaa ccaattgcat cactagaatt aagtaattta tttaatcttt
     1021 cttcttcaaa atttaaagtc attgcttctg taagtctaat agataccttt tcttgcacat
     1081 cttcactact aattaatgtt acaagtccac tcccactctt aacagcagat tcagttgcta
     1141 tgaaacttgc accataaaat ccttttgaac ctgcaactat agatactttt ccaaaatcac
     1201 ttttaaaacc atgcttatct cttcttctaa tattattttt tacaaaagtc ttatcagtta
     1261 tgaactcttc atcatggtat tttcttgtaa taaaatctgg aacacctatc ttttctacaa
     1321 ctacatcacc tataagtgtg ctagtgtcat agtttagaaa acctctttta taaaactcaa
     1381 agctaatcgt tttgtttgct ctaatacaag ttcctaatac atttccatta tcgctatcta
     1441 tacctgaagg aatatctata gaatatgtat ttttgctatt atcgtttact atgtcaataa
     1501 cttctttaaa aataccagtt atatcacgtt ttaatccagt cccaaatata gcatctacta
     1561 caacatcact tttagatact ctatctttaa aaattgttaa atttttattt tttataatat
     1621 tttgtttctc aactttaata attttatcta cttcattttc atctccataa tttttttcac
     1681 ttaaatcatt tttattaata tttataatat taattcccat atttaaaagt acattgtaat
     1741 ttattttaga gcattcactt agtttttcaa gattacctac taaaaatacg tttacttctt
     1801 ttccatatgt atttagatgt cttgctattc caagaccatc tcctccatta tttccaacac
     1861 cacatacaac tgtataacta ttatacatgt ctatatccat atttttcatt gcagatataa
     1921 ccgcattttc catcaataca atcattggaa tccctacttc ttctacacat gcagtatcaa
     1981 catttctgct taatttagat gtacctatta gcataaatta tcactcctat aaagttaaag
     2041 acttcttaaa tacttaccaa aaatatctat tgaaatcttt taatcccata aatatcataa
     2101 ataaaatcac taaaactaag agttttgaaa taatttaaaa taacaaaaaa ttttgaatta
     2161 aaacaattat ttaatttatt ataccatata atttaagtta ttatgttgat ttaaaaccta
     2221 tttacactaa ttatctaaat atctctttat ttttataatc ttttatgact tatttatatt
     2281 tttcattttt ataaaaaaac aataattaag aatataacta tattctacat gtaaaaattt
     2341 ttttatttaa actcaatttt ctgataactt gataattttt caaaatttat agttgacttt
     2401 tattttttac tgtattaaac taattactat acaaaacaga aaacaaaatt ttcaaaatta
     2461 ttaaattaaa aggagaggta aatatgaaga ctttattaaa caaacaagta cgatttttta
     2521 gtagccaatt tagttagggt ttgtacctaa gttaagacaa attgtcataa catagataca
     2581 agccccttag gattatgatt tttaaggatt gtatctatgt tggctatagt aattgttgtt
     2641 aaagttttta ttttatcatc ataatataga gccacatgga ttatactgtg tggcttattc
     2701 gaatttttgc catacagtta tatatactgt gtggtttttt tatatcaaaa tcatttttta
     2761 aaaccaaatt taaaaaacca tttaaaatat tttcaaaggg gtgttttatt tatgtcaggt
     2821 attatatctg taatgacgca aagtttaatt ttatcgatta tggcactggg agtttatata
     2881 acttacaaaa tcctagattt tccagacatg tctgctgatg gtagctatac aatgggtgct
     2941 tctattgtag cattttctct tactaatggt atttctcctg tagtagcaac tttaatggct
     3001 atcttatgtg gttgtactgc tggtcttgtt acagggattc ttcacattaa gtttaagata
     3061 tctaacttac tttctggtat tttagttatg ggaatgctat actctatcaa tttaagaata
     3121 atgggaaaat caaatattcc tctatttagc tttaaacact tattcaatgg ggaaatatct
     3181 cctatagttt tagccttagc ttttgtattc atctgtaaaa tccttttaga tttattccta
     3241 aaaacaggtc ttggatacac tttaaaaggt gttggtgata actctcaaat gattaaatct
     3301 ctgggtataa atatcggttc tataaaaata ctaggactta tgatttcaaa cggacttata
     3361 gctttatctg gtagtcttat ggctcagttc ttgggattct cagatgtaaa tatgggtata
     3421 ggaactttag ttcttggaat tgcctctatc ataattggta ttacactatt taagaaattt
     3481 acttttatca aagatactac tgcaataatt gttggctctt ttatatacca attcactata
     3541 tactttgcaa tgagtttagg aatgttatca actgacttaa aactaataac tgccatagtt
     3601 ataatagctt tcctagctac tggtaactta aatatttcac taaaaaaaac aaatgcaaag
     3661 ctagtaccaa aaataaatca gaaaaaagag gtgttgtaga tgttacaaat taaaaatctt
     3721 tcaaagagtt tccctaatcc atatggtgaa cctaatacta tctttgaaaa tttatcaatc
     3781 gatatagaag atggtgaatt tgtaagtatc ataggtagta atggaactgg taaatctact
     3841 ttattaaata taatatccgg gcttataaaa gaatcttctg gtcaagtatt attaaataaa
     3901 cataacttgt ctaatttagc tgaacacaga agaactcaaa tagtaagtag agtttttcaa
     3961 gacccaagtc ttggaacttg cccttctatg actgttagag aaaacttgtc tttagctctt
     4021 aacaaaggaa aactattaaa tctaagaaaa tgtcttcgtt acaaaagaga tttcttagaa
     4081 aatttattgg aaggtgtatc tcttgattta aaaaaatacc ttgatgttca agtaaaattt
     4141 ttatcaggag gtcaaagaca atcattatca ctaattatgt cgtgcttaac aagtccatca
     4201 gttttattac ttgatgaaca tacagcagca cttgacccaa aaacatcaaa tgaagtaatt
     4261 gaattgacta ataagattgt aagggaaaaa aatataacta cacttatggt tactcacaac
     4321 ttaaaacacg ctcttcatta tggtgataga ttgataatgc ttcataaggg tgaagttgta
     4381 ttagatgtaa aaggtaaaga aaaagaacaa ttaacagttg aagaaatctt agaaaaattt
     4441 gaatatgcag tttaaaataa tatatctatt gtattaaact cggctttaaa ataaagttaa
     4501 aattacaata attatggtta ctttgtaata attcatatga atatatgaac atatataata
     4561 ataaattttt gagggggata taaaaatgat taataaaaaa agattggcaa gtttgatttt
     4621 agctggtgct cttagtatat ctatgcttac agggtgttca caaggaggag actctggtaa
     4681 ttcaaaacaa gaaagtaatt ctaaaggtaa agaagttaaa aaaattggta taactcaact
     4741 ggttgaacat ccagcattag atgccactag gataggtttt gtaaaagcat tagaaaagaa
     4801 cggatttaaa gatggagaaa atattgatat agacttccaa aatgctcaaa atgacatgcc
     4861 tactacacaa agtattgcga gtaaatttgc atctgataag aaggacttaa tatttgctat
     4921 atctactcca tcagctcagg cagcatttaa tgctactaaa gatataccta tacttattac
     4981 tgctgtgagt gaccctgttg ctgctggatt agttaaaact cttgaaaagc caggcacaaa
     5041 tgtatctgga acatcagatt ttgtttctgt agacaaaggt ttagaacttc ttaaaatatt
     5101 tgctcctaag gcaaaaacta taggagttat gtacaatact agtgaagtaa attccaaagt
     5161 tcaagttgat gctttaaaag aatatgcatc taaaaatgga tttaaagttg ttgaaaaagg
     5221 tataactact tcaaatgaag ttaaccaagg tatttctagt ttagttggca aaatagacgt
     5281 attatatgtt cctacagata acttagtggc ttcttctatg cctatagtct ctaaaatagc
     5341 tactgagaat aaaatacctg ttatagcagc tgaatctggg ccagttgaaa aaggtgctct
     5401 tgcttgtcaa ggtatcaact acgaaaaact aggttacaaa acaggtgaaa tggcagttaa
     5461 aattttaaat ggcgaatcag tatctgatat gcctgtggct acatcagatg atacagatat
     5521 cattgttaat gaagatatat taaaagcttt aggtatggaa aaaccttcta atgaaaatat
     5581 ttcttatgta aagactaaac aagaatagaa agatattttc tatatacgat taatatgcct
     5641 gtttctataa attaatttat agaaacaggc atatattttt attttaatgt attatctatt
     5701 taaagattat atgtaaatgc tcttaatatg attgtataaa ttttacttgt ataaacttta
     5761 tacaatcata tttattcttc tattttcttt attactctac atggatttcc aacagctaca
     5821 gtatttggtg gtatatcttt tgtgactaca cttcctgcac ctattactac attatcacct
     5881 atagtaattc ctggtgttat gattactccc ccacctatcc atacattatc acctattttt
     5941 acaggagaac catactctat gcctgaattt ctaagttgtg catcaattgg atgataggct
     6001 gtatagattt gaacgtttgg agctaacatt acattgtcac ctatttcaat tttacataca
     6061 tctaaaaaaa tacaatcata atttgcaaag aaattttcgc ctacatgtat gttatatcca
     6121 taatcacacc taatgttttg ttctacattt atttgcttgc caactgaacc aaatagttgt
     6181 cttaaaatat cttctctttt ttcatattca tcctctaaag tattattaaa caatctagtt
     6241 aattttttac aatactctct ttcttttact aacaattcat catttgcata gtatcctttt
     6301 ccagacaaca ttttttcttt ttcagtcata tagtttactc ctttttgtta taattgtatc
     6361 taaagcaaaa taaatattcc tgtggaaaat ctaaatatat tctgtcacct ttcttaaatc
     6421 tcattttact tttttccaat tctatttgaa taggtacaca ttcgttagat aaatcagttt
     6481 ttctaacata tatagtatat gtaaaaggat tttctacaat gttaatcaat tcgaaacata
     6541 acttttcatt taaatcttca ttagaatcca agaccttaat atgatgctcc ctgattccca
     6601 catattctat attatcacct atttctctat ttaatgtcaa ctcgcatccc cagtctgtag
     6661 cataaatagt atttttatcc agtctattta atttggatat atttttacac cctgttatct
     6721 tagcttctat aaggtacttt ggagattcaa agagctcttt tttaggtctc tttggaagac
     6781 cttctccttt gttgtacact ataatatcat cacaaactcg gtaagcttct tcaatatcat
     6841 gagtaacaaa tagtatatct cccttgtaat cttttaacat attcataagt tctttttcca
     6901 tattacttct taaatgatgg tccagtgctg agaaaggttc atctaaaagt aatatatctg
     6961 gagatgttgc caatgctcta gctagtgcta ccctttgttg ttgaccacca gataattgcc
     7021 aaggatatct cccttcaaac ccatttaatt taagtatatc taagtacctt gctactattt
     7081 cctttttttc actcttactt agttttaaaa gcccaagttc tatattttga cttacagtca
     7141 tatgtggaaa caaagcatag ttttgaaata aaaatcctac ctttctatct tgtgttgata
     7201 aatttatctt cttttcagaa tcaaatagaa ctctatcatt taatacaatt ttacctcttg
     7261 ttggtttctc aagccctgct atacatttta atgtcatact ttttccagaa ccagattccc
     7321 ccaaaaaccc cagagtacct ttttcttgtt taatttctac ctttagttta aaagaggata
     7381 aatctttttc aatatccacg tataagctca actatccaca cctctttcct ataatttttt
     7441 gctgaaattc cgaccaataa tttagtaata aaatcattgc tatagatatt gctacaataa
     7501 tcataaccca catcatagct ttattcatat ctccgccttc aacagcaaaa aatatagcta
     7561 ttggcatagt ttgagttttt ccaggtatat ttccagctat cataagtgta gcaccaaatt
     7621 cgccaagagc tctggcaaac gacagcacca atccaccaat tattccaggc catgctaatg
     7681 gaactgcaat cttaaaaaat actttccatt cattaagacc taatgttcta gcagctgaca
     7741 gcatattatt atcaatctgt tcaaaagctg accttgaagt tctatacatc ataggaaaag
     7801 acaccactac tgctgaaatt acagttgctg tccaactaaa tattaaagtc ctatcgaaat
     7861 ttaataaaaa ctttcctata aatccattct tcccacatag taataataaa aagaaaccta
     7921 caactgttgg tggcaaaatt agtggaagtg taaatagtcc atctataaga cccttccact
     7981 tacctctata gttagccata atatatgaca caaatattcc tattacaaaa gttattattg
     8041 ttgacaaaat agatgtctta agtgatatcc ataagggtga ccaatctgtt cccatctttc
     8101 cctccttaat tttaaatgga aatagtgaaa ctaataactc tattaatttc actatttcca
     8161 ttttattata atacacaatt attttcaatt ttcatttatt tatatataat ttatacttaa
     8221 ttacacataa tttatttata agtttattct acttttttat atccaaattc ttcaaaaatc
     8281 ttttgtcctg actcacttaa taaaaattct tcaaatttct tagcagcatc tacgttttta
     8341 ctagctttaa ttactgaaac tggatatgta ataggtgaat gagttttttc gtctgttttt
     8401 tcaactactt ttatcttatc attattgaca gtatcactga agtaaacgaa tccaacatca
     8461 gcatttcctg attgcaccca tgctaaaact tcttttacat cttttgcaaa tactagctta
     8521 tcttttaact tatcttttaa atttaaatta gttaacacct catctgcata cttaccagct
     8581 ggaacacttt caacttcacc aacagcaatt ttcttcactt tatctgtagt caaatctttc
     8641 atacctgata cactactgtc agcagaagaa ataagaacta aatcattttt tactaaatct
     8701 ttcatagtat cagaaactaa taacttctct tcatccaaag ctttcatttg tttttgacct
     8761 gctgaaataa ataaatcaca aggtgctcct tgttctattt gttgttgtaa tgagcctgat
     8821 gctccatagt taactgtaag ttttacattt gaatcaactt ttttatattc ttcttcaatc
     8881 tttgccattg cttcttttaa acttgcagca gccgatatat ttaactctac tgaatcacta
     8941 cttttagttg ttttttcttg ttttccatca tctgttttgt cttcattact attgcaccca
     9001 actgtcccca aagttaaaca tgcaactaat cctaatattc ccaatatttt tttcataaca
     9061 atctcccttt ttgtaaattt gatacacatt ttaatatgtt ttattatgtt ttatcatgtt
     9121 ttgttatata tagtttaatt atatatacat gataataaat tttcaagtaa tttatttaca
     9181 ctacttttaa atattttgct acattttcct ttttttaatg ttaaaataac cttatatgga
     9241 ggtggattat ggactcatta tcatctttga cacctgtcga ggtagctgaa ttattaaaga
     9301 taacaaaaaa tactgtatat gaattaatca aaagaggtga acttccttct tataaagtag
     9361 ggaaaaaaat aagagttgat actaaagatg ttgaagaata tatcaacagc caaaaaactg
     9421 gtaaagtaaa ggatacacat ttcaacaata aaaatataga tataaataat aatttcaaaa
     9481 ttattatttc tggtcaagat gtaatattag acattttagc taggtctgtt gaaaaaaaat
     9541 tggatggaat aagtacattt cgctcttata taggtagtta taatgctttg tatgagttat
     9601 ataatggcag agtttctata gcatcatccc atctatggga tttcgaaacg gatgaatata
     9661 atagcacttt tgttaaaaag ctattgccag gaataccatg tgtacttata aatctagctt
     9721 atagaatgca aggtttctat gttgctaaag gaaatcctaa agagataaca acctgggagg
     9781 atttaacaaa accaagtata accattgtaa atagggaaaa aggttctgga actagggttt
     9841 tactagatgg taaacttaga cttctaaact ttaatggtaa acatataaaa ggttatgata
     9901 atgaagaatt atcacatctt ggagttgcaa gtactgtttc tcgtggaata gctgatgttg
     9961 gtttgggaaa tgaaaaagct gctttacaag taaataatat cgactttatt cctcttcaca
    10021 aagagcgcta tgatttagtt attaaaaaag aagacttaca aaatccagtt taccaaacta
    10081 taattaacat aataaactct cctgagttta aagctgaact tcaaggtctt ggcggatatg
    10141 atttaactga tactggaaaa actatagcaa aagtttaaga ttaaaatcgc aatctatttt
    10201 ataagattta taatttttca caaaatttat aacttaaaca cgaaataaat tgcgatttta
    10261 gtgtctcaaa aaatttatgt ctttcatttt atcatccaat acaaaatata atataattaa
    10321 ttgtgcaatc atattatatt aatatatatt tacataatag tatttttctt taatgtagat
    10381 taacatatga tatgttttaa acatcaacac ccttgctttc ttatatatat gtggcaacac
    10441 atatgagttt aataaattat atacaatgtt gctttcttct tgtatttttt caaccaacaa
    10501 gttatttata aaattacctt tctcattttc aataatctca ctccaattta tcttaacttt
    10561 taatatatat cttcccacta ggtatacaca ctctacaata aataataaaa atacaataca
    10621 gtcagtacct gttgtcttat ataaattacc cgatactaat ggctttgcga agaaattata
    10681 aaaattcaaa gtatcacccg tataatcaaa aagtattaat agcactccta gaactctcaa
    10741 tactttaata ttcaacatgt ttttatcttt attatttttg tttttcatat atactacctc
    10801 tcttttcttt agatttgatt gtaatctaat actaactctt ttatttgagg tataaatgaa
    10861 tttaattggt agttattttc atttttacta gaaattaatt cacattacta aacaaaactt
    10921 atatttaaac ataaaaatag cctatctatt ttttaataaa tacaaaaata aataagctac
    10981 taatattcaa ttaactttta tcacttaaaa cattactatc tataaattta aatattttat
    11041 tgaagtatac tgtactatca tgttcaaatc catcaacatg accaatacct tctataagcc
    11101 ataggtcttt tttatcatgt ttaactaaat catacatttt cttgctattt tgataattac
    11161 aaacagtatc ttttgttcca tgtataaaca gtatgggtac attagatttc tcaactgact
    11221 catatggttt tacatcactg taccaaaatc cagatttaaa cttattatat aaatctccac
    11281 aaatcaccac ataattgatt aatatatctg gtattcgttt ttctagtact cccattctaa
    11341 ttgcatcttt catttcagaa taaggagaat ccagtatata aaaatcgacc ttatcatctt
    11401 ttgaatttat ctctgaatgc atagcagctg tccctgctcc catggaaaat ccatgcactc
    11461 ctaatcttcc ttcaggaaat ttatttttta caaatttaac tagactatct aaatcatacc
    11521 tttcataaag accaaatgtg taattatctc caccactatt accagtattt ctttgattat
    11581 aaactaatac attataacct ttatctaaat atctatatgc tacctttaac atttcatagt
    11641 aactacttcc aattccatga acaagtatca tagtatctct tgtctttata tttgaagtta
    11701 taaaaagact ttcaagcttg taattattag aactttgtat cattaattca gatgtcttgt
    11761 agttttttaa tttctccagt ggtttatctt ttctctcaga aaatattttt actatatctt
    11821 cgttttttat taactgctct aaaccaaccg aactattata cactaatttg ccaaatatat
    11881 aagatacaat taataggatt aatagaaaca caattgatat aattataaat ttatttctta
    11941 aactaataca atttttaaaa ctatttttca tatttattta ccatacaatt ttcctatatt
    12001 atattttcta agt
//
LOCUS       DAELXX010000112        11963 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.113, whole genome shotgun sequence.
ACCESSION   DAELXX010000112 DAELXX010000000
VERSION     DAELXX010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11963
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.113"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            103..816
                     /locus_tag="KPH29_003016"
     CDS             103..816
                     /locus_tag="KPH29_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017208807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HBG1468593.1"
                     /translation="MNKISKRGFDDSDKRWFSSKELTRLIKAKEEIEWLLNRDYKIDP
                     VVTFVGDRYQFSIRQRDALKRAVCTDEKNIIRQSKRLFLDKIKEDTVNIDGFNLIISI
                     EVALSGGTLVIGSDGNIRDLAGLRGTYKIIDKTEEALNLIGKFFNKYNAQNLKFYLDA
                     PVSNSGNLKYRILEHAKTWGIETEVELVKNADVVLEKLDRVVSSDAVIVDKCISYFNV
                     ARGIIEEYIKECNIINLNK"
     gene            934..1803
                     /locus_tag="KPH29_003017"
     CDS             934..1803
                     /locus_tag="KPH29_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG1468594.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANVNLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKDLVSVSEYAWSNLNLREDKSITSNIITV
                     IPEKSRVEVLQVDGDWSKIVYDDKIGYVFNYFLSTDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(1958..3103)
                     /locus_tag="KPH29_003018"
     CDS             complement(1958..3103)
                     /locus_tag="KPH29_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG1468595.1"
                     /translation="MSTYYVPPINLLGKGCLNEAKDSIKSLGTKKAFVVSDKFLVSNG
                     TVKKVTDILSQIDVDFVVYDEVKQNPTVTNVDKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIVAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAVEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKEKIIELFMKIS"
     gene            complement(3436..4212)
                     /locus_tag="KPH29_003019"
     CDS             complement(3436..4212)
                     /locus_tag="KPH29_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBG1468596.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADVINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
     gene            4794..5768
                     /locus_tag="KPH29_003020"
     CDS             4794..5768
                     /locus_tag="KPH29_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBG1468597.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
     gene            5866..6171
                     /locus_tag="KPH29_003021"
     CDS             5866..6171
                     /locus_tag="KPH29_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG1468598.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGNEISFKLKDKTVKTITALDNI
                     TIYHKLAIKDMAQGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            6327..7487
                     /locus_tag="KPH29_003022"
     CDS             6327..7487
                     /locus_tag="KPH29_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG1468599.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNMD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            7537..8439
                     /locus_tag="KPH29_003023"
     CDS             7537..8439
                     /locus_tag="KPH29_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBG1468600.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESIFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            8519..9412
                     /locus_tag="KPH29_003024"
     CDS             8519..9412
                     /locus_tag="KPH29_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HBG1468601.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNKLNIC"
     regulatory      9465..9646
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            9874..11004
                     /locus_tag="KPH29_003025"
     CDS             9874..11004
                     /locus_tag="KPH29_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG1468602.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTEEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     gene            11076..11921
                     /locus_tag="KPH29_003026"
     CDS             11076..11921
                     /locus_tag="KPH29_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBG1468603.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     KLIKK"
BASE COUNT         4505 a         1379 c         2131 g         3948 t
ORIGIN      
        1 ctaaatggat aagataatgg tattaaaaat tatcttaaaa ggtgaaataa tctgtattta
       61 atgtagtaca gttatatctt taaagcaagg tggtgaaatg atataaataa gatatctaaa
      121 agagggtttg atgatagtga taaaagatgg ttttcaagta aagaattaac aagacttata
      181 aaggctaaag aagaaataga atggttatta aatagggatt acaaaataga cccagtcgtt
      241 acttttgttg gtgatagata tcagttttct ataagacaaa gagatgcatt aaaaagagca
      301 gtctgtactg atgaaaaaaa tattataaga caatcaaaaa ggctttttct agataaaatc
      361 aaagaagata ccgtaaatat agatggcttt aatttgataa tatccataga ggtagcatta
      421 tctggtggta ctctagtaat aggaagtgat ggaaatataa gagatttagc aggtcttagg
      481 ggaacctata agattataga taaaacagaa gaagctttga atctgatagg taaatttttt
      541 aataaataca atgcacagaa tcttaaattt tatttagatg ctccagtttc taattcagga
      601 aatttaaagt ataggatatt agagcatgcc aaaacttggg gaattgaaac tgaagtagaa
      661 ttagtaaaaa atgcagatgt tgtattggaa aaattagata gagtagttag tagtgatgca
      721 gtcatagttg acaagtgtat aagttatttt aatgtggcta gaggtataat tgaagagtat
      781 attaaagaat gtaatatcat aaatttaaat aaatagataa atagaggtga gaatatataa
      841 gtatatgtag caatattatt agtaatacaa gtaaagtttg aaaataggag aacgaataca
      901 tatcgaattt cataaaatgt tgtaaaaaga catatgagag gatggatatg tgtgaaatta
      961 actaaactaa atattaaaaa atatagagca cctatatata aatatgcttt agcaaatgta
     1021 aatttgaggt ctgctaaatc tactaattca agtatcataa cagtaatacc tcagggagct
     1081 aaaatggaag tattggatga agaagatgac tggattaagg tgatgtacaa ttctcaagaa
     1141 ggatatgtgt ataaggattt ggtatcagta agcgaatatg cttggagtaa cttaaactta
     1201 agagaagata aatctataac ttccaatata attacagtaa ttcctgaaaa atctagggtt
     1261 gaagtccttc aagtagatgg agattggagt aagattgtat atgatgataa aataggatat
     1321 gtttttaatt attttttatc aactgatggg aataaaccaa atgaattaga ttataagtat
     1381 ttttatactg acatgattaa atttgttaac gaaaataata ttaaaagtac tagcgattat
     1441 ctaattgtaa cagaccttag aaataaatat acttatattt ttaagaaaga taatggaggc
     1501 tgggggcaac tttacaagtg gcaatgtact ataggcaagc ctgagactcc aactataaca
     1561 ggtatatttt atataagtgg aagaaaacct tcttttggaa cggatgagta ttcagttaag
     1621 tatgctacta gaattaaagg tggatattac tatcattctg tgctatatga ttcaacagga
     1681 agttatataa tagatggcag acttggtgaa gctcttagtc atggatgtat aagattaagt
     1741 acggaaaatg caaagtggat atatgataat ataccagata caacaacagt tataattcat
     1801 taagaaaaat tttgagtttt tattgggata atgattctca aattaatagg attatatatt
     1861 aacttcttca taaagagtac tatttaaaaa gtgtaataag agagtgctat taacaaaaca
     1921 aagatatttt gaggcactct cttataaata tacataatta agatattttc ataaacaatt
     1981 ctataatctt ctccttatca aagaaaacag gattagcacc agcacaagca tctttcatag
     2041 agttttctgc aagcaattca aaattaggat tatcaacacc tacatctttt aaagattttg
     2101 ggattccaac ctcttcagaa agagctttta tcttatctat tacaaagtca acacactctt
     2161 tatctgattt attatttaca tccattccaa taacttcagc tattggtcgg aatttggcag
     2221 gggctgattt agcattttcc tcctcaacta ttggaagtag catggcatta catactccat
     2281 gaggtaaatc atataaacct cctaattgat gagccatagc atgtacattt ccaagacctg
     2341 cattactaaa ggcaattcca tttaaaaaac aagcataaca catttgttct ctagcttcta
     2401 tgtcattacc atttttaaca gctcttggaa ggtatttaaa tatctgcttt atagcatata
     2461 aggcagttga atctgtaaca tcataagcac cattagctac tacagcttct acagcatgag
     2521 ttagtgcatc cataccagtt gctgctgtta aattagatgg tttgctaatc attaattctg
     2581 ggtcatttac tgtcattgta gctagtgagt tggtatcaac cattatcatc tttacatgtc
     2641 tgtcttcatc agttataaca tagtttattg taacttcagc agatgtacca gcagtagtag
     2701 ttatagctac gataggaaga cattttttag aagttttgtt gataccttca tagtctctaa
     2761 tatctcctcc attagtagct aatatagaga tagctttacc acagtcttgt ggagaacctc
     2821 ctcctatagt tataacgaag tcacaatttt ctgattttaa tattttaagt cctttatcca
     2881 cattagttac ggttggattt tgtttaactt catcataaac aacaaagtct acatctatct
     2941 gagataaaat atctgttact ttttttacag taccatttga aactagaaat ttatcactaa
     3001 caacaaatgc ttttttagtt cctaatgatt taattgaatc tttagcttca ttaagacatc
     3061 ctttcccaag taaatttatt ggtggtacat aataagtgct cataactaaa atctcctttt
     3121 attttcattg atttttatgt aaaaatatat atctaacaat tttcactaat ttactcataa
     3181 tatatcgtat cttagattga gatagggtta ctggttctta tataagaact taattcgtaa
     3241 ttacgattat gattagaatt taacatagta tttttatcat gtcaataaaa atataaaaaa
     3301 tattataaaa aaaacaaagt tactgtagtt aaatcagcaa ctttgttttt atagtataaa
     3361 tcaaataaat acattagttt aaaacaaaat ttttaattat tatgttttga tatcttccat
     3421 atacagaata ttaatctaat ttattacaat gtcttcatta cgttcttcca tcattgattg
     3481 aatatttttc tgagcattta agagtaattt ttttatcagt tctatttttt cgtcattagc
     3541 tctaaataaa ggcagtgtta cactaacagc agctcttata catccatttt ttcttattgg
     3601 tacggcaaga cattttaggt gttccattga ttcttcacac tctgaagcta tttgtgttct
     3661 ttgaacttca agaagctggt cgtataaaac atcaaaatct gtaattgatt tttctgtaaa
     3721 accttttaga ccatctggat ataagcttat tagtttttcc ttgtcaaaat cacataaaag
     3781 agcttttcca agacctgtac aattagcagg tatttttttt ccaacataag aaataagtct
     3841 aattggttct ggagaatcta cttttccaat ataaagaact tcattattgt caagtatacc
     3901 aagctgacaa gtttcagaac aggaattaac tatattagtc atttcgtcaa ttatatagtt
     3961 aaaaatagtt cttttttcaa aaaatgaaga ccctaatgtg taagaacgta tccctataga
     4021 atatttggat gtgtttttat caatagtaat gtattttcta gcattaagtg tatgaactat
     4081 aggaaagatg ctactttttg gagcattgat aacatctgct atttctgtaa gagtatatcc
     4141 attttcggat tctgctaatg cttctaaaat atctaatatc ctagctgttg ggcgatgatt
     4201 atctgaagtc ataaatttcc ccctcttttt attaatttaa agtattagaa tatgttgatt
     4261 ttagcacact atacgatatt ttacaatgta gaaaataaat atgtaaattt atatataaat
     4321 aagctcttga tttagataca gatagatatt tttttagact gtacattaag gaataactaa
     4381 tttccaaaat atacctaaag aaagtaagtg atatttagtt atataattta agtaaatata
     4441 tgcaatagtt atttttatat gtaaatatca aaatattatg aatttatatt ttatctattt
     4501 tttacactgt ataaaaactt tatagttttg aatactatat aattttaaat acgctgtatt
     4561 tttgcatata aggaagtcat ttgataatat acaaggtatt atcagaaagt ttgaaaaaaa
     4621 tttgacaaaa atttattttt tttattgact cataaatagg aaaatgctat cattaattca
     4681 aagattcgta attaaaaact tagttcttaa atacgaaccg aaacggccgt tagaattaaa
     4741 gctaaaaact taataagagt aattaaatat catgttttag gaggattaac tttatgataa
     4801 tggattttat taaaaaaatt cctgcaggta tgatgatagt gccaatgttt atagcagctt
     4861 tcattaatac cttttgccca gatattgtac aaataggttc gtttacgaca gcagtatttt
     4921 caagtgttgg gtctgcaact ataattgggg ttcaactggt atgcttggga actcagcttc
     4981 aatttagaga aatgccaaaa gttttaaaga gaggtggtat acttcttggt tctaaatttg
     5041 ctataggtgc tttgataggt atacttatag gaaagctatt tggaatggat ggtgtccttg
     5101 gtcttacaac attggctgtt ataagtgcag ttacaaatag taatggtagt atatatcttt
     5161 cattgatgac ttcatatgga gatgaaacag attgtgcaac tatgagttta cttactttaa
     5221 atgatggacc attttttaca ttaatagctc ttgggacttc aggtcttgca aatgtgccat
     5281 tgttgtcatt attagctgca atagtgccaa tacttgttgg tatgataata ggaaattttg
     5341 acaaaaaaat gaaagagttt ttagagccag ggtgtaattt actcgtacct tttgtagggt
     5401 ttgcacttgg tagtagcata aatcttgcaa gtatactaaa aggaggttta tcaggagtat
     5461 tacttggatt aatatctgta tttgtaggag gtatatttat agtattctgt gataaattta
     5521 taggaaaacg tccaggatat gcaggatggg ctgtagctac aaccgctgga aatgcaattg
     5581 ctgtacctgc tgctgttgca ttagttgacc catcatggca accatatgtt gcaacagcta
     5641 caacacaagt ggcagccgca gtagttgtca ctgcaatatt agtacctctt ataactagtt
     5701 ggtgggctaa gaagtatgga tgtcctaagt tcccaaaaca aaatgaggat ggtacaataa
     5761 cagcttaatt tatattacag tgagttgttg gtaaaataat aaattcttta attagatttt
     5821 aggatattaa taaaattata ttatttataa aggagagcaa gaataatgat aaatgcagtg
     5881 ataattgatg aaaaagataa tgttgcagta gctattgaag caatagctaa aggtaatgaa
     5941 attagtttta aattaaaaga caaaacagta aaaacgatta cagcactaga taatataact
     6001 atttatcata aattggctat aaaagatatg gcccaaggtg ataaagttac taaatatggt
     6061 gaacatatag gagaagcaaa tcaggaaata aaaagtggtc aacatgtaca tattcataat
     6121 gttagaagtg ttagagagga tttggataaa caagttatga ctaatatata aaataatatt
     6181 tatagagtta taaaagttgt attcattgct tttagtgtca aatagttttt aaataggatt
     6241 ttagctgttt gattagtgag tgtaataaaa attataatat ataaaaatta taaaaagtat
     6301 atagactatt ttaggaggaa atcagtatga aattttatgg atataaaaga ccagatggaa
     6361 gagttggtat aagaaaccac atattaatat tgcctacgag tgtgtgtgca tcagatacaa
     6421 ctagaataat agcatcacaa gttcaagggg cagttacctt taataatcag aatggatgtt
     6481 cacaagtaca ttcagaccaa caattaacta tggatgttat ggctggttat gcagcaaatc
     6541 caaatgtata tggtgtaata gttgtatctt tgggatgcga aaattgccaa aatgatttgg
     6601 tagttgatgc aataaaagag agaacaaata agccaataaa aactcttgta atacaagaag
     6661 aacatggaac tttaaaaact atagaaaaag ctgttcgtta tgctagagaa atggcacaag
     6721 aagcttcttt gcttagaaaa gaagaatttc caatatctga acttatacta ggaactgaat
     6781 gtggtgggtc tgacccaaca agtggattag cagcaaatcc attaataggt gaattaagtg
     6841 ataaacttgt cgatttagga gcaacatcaa ttttaagtga aactactgaa tttataggtg
     6901 cagagcatat actagctagg agagcagtaa atgaagaagt aaaagaaaaa atattacata
     6961 tagtacatag atatgaaaat tcattaaaat tggttggaga agaagttaga gaaggtaatc
     7021 catctcctgg aaatatagct ggtggtttaa caagtttaga agaaaaatca cttggttgta
     7081 tacataaagg aggtcataga caaataagtg aagtatatga ttatgccaaa caaattgata
     7141 agaaaggtct tgttataatg gatacccctg gaaatgatgc ttcatctgtt gctggtatgg
     7201 tagcaggagg agcacaaata gtggtattta gtacaggaag aggaacacca tcaggaaatc
     7261 caatagcacc agtaataaaa ataactggaa ataaaataac ttttgcaaat atggaagata
     7321 atatggattt tgatgctagt ccagttatct atggtccaca aacaatggaa gaattaacag
     7381 atgatttgtt aaatatggtt gtagatgtag caaatggaaa acaatcaaaa gcagagtccc
     7441 ttggatatac tgaaatggca atagctagag tatgcaacta tgtataatct ttaagtaaga
     7501 aaataaatac atagaatttt aataaagggt gagattatga agtttgtaag ttttactgaa
     7561 ggagaaaatg aatgtaaaag aactggagtt ttttctaaag atgaattatt tattatagat
     7621 gttaactctt taaatctcag tagaaatttt aaggatttaa atgaattaat tcaaaatgta
     7681 tctagtgaag atattattaa gttgaaaact attataaatg aaagtatatt ctataattat
     7741 gatgttttta aaagagagag atgtactgta catgttccta tagaaaaacc aatacatgat
     7801 atcttatgtg tgggagtgaa ctataaagac catcttgaag aaactcaaac tcactttgat
     7861 gatagttttg aagagccaca aaaaacggtt tattttacaa aacgtgtgac aaaggcaata
     7921 ggtccagaag atgagataga tggtcatttt gagataaata atcaactaga ctatgaagtt
     7981 gaacttggag tagtaatagg taaaacaggt gttaatatta gaaaagaaga ggtagaagat
     8041 tatatctttg gttacactgt tataaatgat atttctgcta gaaagttgca aaaagaacat
     8101 gttcaatggt ttagaggaaa aggattagat acatttacct ctttaggtcc atgtatttta
     8161 cataaaagtg cagttccttt tcccataaaa cttaaagtat gtagtagggt aaatggtgaa
     8221 gaaagacaaa gttctaatac aggattattc atatcagatg tttcagacat agtttcagaa
     8281 ctttcaaaag gtatgacttt agaggctgga gacattatag ctactggaac accttctgga
     8341 gtagggatgg gattttcacc tcctcgatat atgagtagtg gagatgtagt tgaatgtgag
     8401 attgagagta ttggaatttt aaaaaattac attaagtaaa tgttttaata taattaatat
     8461 taaagctaaa tctgatgtat taatgaacat attaaaataa attttaggag tttaaattat
     8521 gaaagcagaa tttttgacac cagttgttac aatatttgat gaagaaggaa atttagataa
     8581 agaaggtaat aaaaaagtgt acgaccatct tatagatgga ggtgtagatg gactagtcat
     8641 aatgggaagt actggagagt tctttaatat gtctatggat atgcaaaaag aacttataga
     8701 tttagtaact agtcatgtaa ataagagagt aaaagtgttt ataggtacaa gtcgtatgtg
     8761 tataaatgaa tctatagaac tttcaaacta tgcacataat agtggagcag atggcgttat
     8821 gataataagc ccttattatt tctctcttga tgatgatagt attgagttat tttatagtga
     8881 aatagctaag aatacagagg ctgaaatata tttatacaat tatccagata gaactggtta
     8941 tgatttaagt ccagaactta cattaagact tgcaagaaaa cataaaaaca tagttggata
     9001 taaggataca gtaactttga tgggacatac cagagattta ataaatacta tgaaaataga
     9061 gttcccagaa tttaaggttt atagtggtta tgatgaaaac tttatacata atctaatgtc
     9121 tgggggagca ggttgtatag ggggattatc aaatttaatt ccagaagttt gctccaaatg
     9181 ggctgaagct tacaataata gagatatgga gaaggttata gaaattcaaa aatatattaa
     9241 taaagcaatg gatttttact taataaccaa tccatttata ccagcaatga aaaaagctat
     9301 gaatatcaga gggttacaaa taagtgatta tatgactgct ccatttgtaa aaccaaatga
     9361 aaaacaagta tgtgaaatta aaaaattgat gaataaatta aatatatgtt aaatcaacct
     9421 tgcaaagagg tcaacaataa attacaatta tcttaataaa aattaaatta aaataatact
     9481 agggtactat attagtttaa tagggaaagt gatgaaaatt cactacagcc cccgctactg
     9541 tatacggata cgaaatcaaa atccactgaa atttataaaa attttgggaa gggtgatgga
     9601 ggaaaagccg tgagtcagga gacctgccca gtattatata agttcaattt agggtggaga
     9661 ttgtaattta taataaaatg aagtatcaat tcgtttttgt aaagttgata tatgaacttc
     9721 tccttattaa attaggagaa gttttttatt ttattaaaag gttatactta ttaaagatga
     9781 ctttaggata actttaataa gtatacttta aaaagcatat attattaaag ttttttagcc
     9841 actcataaaa aataagatat tggagtgaaa gagatgaaat ttaaatcaaa tgttttaaag
     9901 cagttttttt taattgctat ggtaattttt atggtaggag gtcttatagg gtgtagtaaa
     9961 agtgacaaag agaataaaca agctagtact ggtaagaaat tagaattaaa atatgctagt
    10021 ggatttagtg ttgaagattt agaagatgga attaaaaaag taactgatgg agataatcga
    10081 gaactgattt taatcccaaa gaagattaaa attcctgaaa aatataaaaa tgctaatata
    10141 gttagaacgc ctgtggacaa tgtattaata gcatctacaa cacaggcttg ttctctgaga
    10201 acaatagatg aactagatag tataaaagcc gtaacaacag aagagcagta ctggactatt
    10261 aaagaaatta aagatttaat gaaagatgga aaaatacatt acgttggttc aaatttagca
    10321 ccagattatg aaaaaatagt agacttaaat cctgacttaa ctatagtgag tagtggttta
    10381 tcagaggctg atacaaaatt tattgacaaa ttaaaagaat tagggcttaa ttatgttgta
    10441 gacaatgagt ataaagagca aaatccattt ggaagaatgg agtggacaag tttaatagct
    10501 gcattttatg ataaagaaga tatggctact agtgaactaa ataaaactgt acaaaaggta
    10561 gaagaagtaa gtaaaaaaat aaaaaacaaa gctaaaccta aagttgtatg ggcatctgta
    10621 tatgaaggaa gtgcttatat agctcctaaa gattcatatg tggataatat gataaaaatg
    10681 gctggtggaa taaatgcatg tgcttcaata gattcaaatg aagggcgagt aagtattgaa
    10741 caattgcatg aagttgcaaa agatgcagat atatttgtat actcatcaag ttcagattat
    10801 gcaccaaatt tagattctat aaaatctagt gcacctgtat tggctgattt ggatgttgta
    10861 aaaaacggaa acctatgggt gtttgctcca gattattatc aatcagtaga taagacggac
    10921 gaattaatag tagatttagt tgagatgttc cacccaggta ctacaggtga aaaaatcaaa
    10981 cattatataa attatgataa atagtacaga gaggacacaa aaagataggt tttatagatt
    11041 ttagttaata taactttgct aagaggagta tacaaatgga ttttatcaat aaaatgagaa
    11101 taaggaatac gacaatattt atagtattaa ttataagtat gatacttact tttttagtgt
    11161 gtgtagcatt tggctctgta aaaattgaat ttaaagaagt aatagatgtt gtattaagag
    11221 gtatggataa tacagaacat tataaaataa tatacaatat aagaatccct agagtacttg
    11281 caaccatggt aggtggagca tgcctagcag tatcaggtat tttattacag atatttttta
    11341 aaaacccaat agttgaaccg tatatattag gagtatcttc aggttctact ttatcagtag
    11401 ctctcatgat tttgggagga tacacattgg gatttgagag tgtaaattca atgagtatat
    11461 ttgtagcagc ttttatagga gctttaattg taatgtctat agtcatttta tttgcaaata
    11521 aggtaaagaa tataacaact cttttaataa taggaattat gaccggatat gtatgtagtg
    11581 gtataacaaa tgtgttacaa tcatttgcaa atagtgataa attaaaagaa tttactatgt
    11641 ggtctatggg aagtttttct ggatttacat gggaaaaggt agaaatactt ataatagttg
    11701 gtatattagg tcttttgatg actgtattca taataaaacc tttaaatgct tttttattag
    11761 gagaagagta tgcaaaaagt attggcataa atataagatt ttttagagtt ttgatagtgt
    11821 ttatatctag tatattagca gcaatagtat cagcatttgc aggacctata gggtttattg
    11881 gacttgcagt gccacaaatt tcaaaattga ttaaaaaata gctacgcaca cttgtgactt
    11941 tagtcatgag ttagtagcta aaa
//
LOCUS       DAELXX010000113        11536 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.114, whole genome shotgun sequence.
ACCESSION   DAELXX010000113 DAELXX010000000
VERSION     DAELXX010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11536
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.114"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(72..497)
                     /locus_tag="KPH29_003027"
                     /pseudo
     CDS             complement(72..497)
                     /locus_tag="KPH29_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
     gene            complement(970..1257)
                     /locus_tag="KPH29_003028"
     CDS             complement(970..1257)
                     /locus_tag="KPH29_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468604.1"
                     /translation="MRLNVTKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNVFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(1277..1552)
                     /locus_tag="KPH29_003029"
     CDS             complement(1277..1552)
                     /locus_tag="KPH29_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468605.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAIGMLKGVDYEPLIVATQVV
                     AGTNFKFICNATSVTNPPHDYLAEIIVFKPLPCDEHNATITSINIIK"
     gene            1740..2559
                     /locus_tag="KPH29_003030"
                     /pseudo
     CDS             1740..2559
                     /locus_tag="KPH29_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418025.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(2818..5385)
                     /locus_tag="KPH29_003031"
     CDS             complement(2818..5385)
                     /locus_tag="KPH29_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBG1468606.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTNQLEDVSKKIGELFHNGEMPQEVSDAIKTAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDAVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSKP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMDGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIALQSEKE
                     LWGLSEWVKQNNSINLYLQNSPAIKIAEDFMSSVMPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAYSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYVSPSKLPEKNKKTISQ
                     APQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRTIKSGQMITVDGLAGTVTFDE"
     gene            complement(5388..5993)
                     /locus_tag="KPH29_003032"
     CDS             complement(5388..5993)
                     /locus_tag="KPH29_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG1468607.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRPSDSSEKMLDETMNIFLHGIMNDGGE"
     gene            complement(6367..7827)
                     /locus_tag="KPH29_003033"
     CDS             complement(6367..7827)
                     /locus_tag="KPH29_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HBG1468608.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRANIFHKAADLLLENIDEIANILVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDRAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
     gene            8158..8736
                     /locus_tag="KPH29_003034"
     CDS             8158..8736
                     /locus_tag="KPH29_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG1468609.1"
                     /translation="MAKSFTEIEKDNIRKRLISECEINWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNGIQNRLISSVDEILSNMPPKEGFAKSLKMLYRLYDKN
                     PFLYSPNNPDFISLINKLPQDMFLQLESNNIQSFYDLIKRHNLKLKIEKEKAFGVCNS
                     LLSSLLVKENIEYNYIEVFDFMVDNLVEHILE"
     gene            9028..10278
                     /locus_tag="KPH29_003035"
     CDS             9028..10278
                     /locus_tag="KPH29_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG1468610.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQNSNIIKTIFTDIKDGIVHIC
                     KENRFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKHLKITNLHISFIICAVMFIPMAVSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYVITVTQLKTPNELLGKVMSILFAGTAIAMPLGQVMYGKLFDLLSDRVYII
                     ILGVGFITLLIAFVSKITLRNENL"
     gene            10439..11008
                     /locus_tag="KPH29_003036"
     CDS             10439..11008
                     /locus_tag="KPH29_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBG1468611.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVDFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAVAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
BASE COUNT         3815 a         2000 c         1589 g         4132 t
ORIGIN      
        1 gtttccctat atgctgacta ttttagctac taactcatga ctaaattcac gagtgtgcgt
       61 agctattttt taatcaatac aattactgca catatagatg cagaattttt aaatacttca
      121 cctaatgaaa ctgctgcact aatatcatgg gctactcccc tattgtcgct aaaacctgca
      181 gtatcacata attttgcatc cattccttct attggttttc tcatatttac aatgtttggg
      241 tataaagttt gagaaacagc tcctgaacca atctctgcca cagggtctaa gcaatcaata
      301 tatgatatac cagtatcttc atcttctact tccatcatta cacatcctgc aagatagtta
      361 attgcagtac ttttgccaca tcctgtccca ccaagaatta gaattaaatt ttgattttca
      421 gccttattta ttgctacttt tccttcattt aataagagtg ttatttcttc taaaagttga
      481 ttgttatttt ctggcattta ttttccccct tggttatata ttttatttaa ataaagttta
      541 atcccttaag tagattacaa agtttttaga ctatatttaa atttgttttt tatttttaat
      601 aaatttgcat tttcactcaa taattattct tctttgttta ttaaaataac tgtgttgtat
      661 aatataattt ttaccaacac agttatttta tttaatgcgc ttattttatt gtattttatg
      721 taatacagtt gtttttttat attatatgtc cactattttc ctatggttat tacatataga
      781 tttttaatta tatttgtacc attaatttaa acattcctat actactgctt aagttttaca
      841 cctataccag cttgaatatt ttatttttat ttgcttaaat agttcaacta tactggttta
      901 ataggtttcc tttagtttaa atagttcacc tatacctact tgaatagttc acttttattt
      961 gcttaaattc tataccaata ctggcttaaa cattatacta ccatgattgt attgtctatc
     1021 ttcaactaca ataaaaacag gttctaaatc tttagtagat gttattttta attctaaaaa
     1081 aacattatca gttggtaata tttttacatc ataattattt aaattattat tcgtgatact
     1141 attaaataca tcaccttgac gttcaaaaaa aacattatca actattacaa aataaacatc
     1201 ttcttttcta ctattttcaa cctctatagt attttgttta gttacattta gtctcatctt
     1261 ccacctccta ttaatattac ttaattatat taatacttgt aattgttgca ttatgttcat
     1321 cacaaggaag aggtttaaaa acaattatct ctgctaaata atcatgtgga ggatttgtaa
     1381 ctgaagtagc attgcaaata aatttaaaat ttgttcctgc tacaacttga gtagcaacaa
     1441 taagtggttc ataatcaacc ccttttagca ttcctatagc ttcattaaat acttttaaat
     1501 cttcttcttc gacagcccta aattttgacc atccaccaac aaaaagactc atataaaaac
     1561 ctcctttata tatatattta tttatatgat agtaccaatt ttcttaatat tcaatatatt
     1621 acgtctaact ggtcaataaa aatgcataac tggataaaaa gacttataaa atagcttaac
     1681 tttacatata ataagattat tctaaaatat gagacatatc ttgctttgaa tatattctat
     1741 acaacaattt tccaggcctc attgccaagt ttaaggtaac tgactttgga ttccaacttc
     1801 ttgatgctaa taaaaaatat tttgatatgt tcaaagataa tgtaaatttt ctattagatg
     1861 atataaattt agaaggttat cttaacaatg tgtataaata tcataatgat tttaaatctg
     1921 ggagatgcgt ttgtttttct atttctccac aaactattaa tggagatatt tttcacatgc
     1981 aagttgtagc tgaatgtata gattgggaag atgataagcc tatatatctt ttaatatatt
     2041 cagatgtaac gacattggta aaacaatatg tctcattatc atttttcatc caagaatttt
     2101 atcaagaaat atgataaaat agctaaaaaa tatgatattc cttctcacct tatagaaatt
     2161 gagattacag aaacaattgt atttgaagat gtcaaagtat ttagtgaaat aataaatgaa
     2221 attcataaaa atgactttaa atgctctatt gatgattttg gaagtggcta ttcttcaatc
     2281 aatatgctta agggtattta tgttgataca ttaaaattag atagagcatt ttttcaatca
     2341 gaagatttag attctcaacg tgaaagaaat ataattgtat ctataatcga actagctaaa
     2401 aagttaaaaa tgaccactgt agctgagaga atagagacca gttttcaaaa agatttcttg
     2461 caagaaataa actgtgatat gttacaagga tacgtgttct ctagaccttt gcaaataaaa
     2521 gactttgaaa aacttatgtt tcaaaactcc tccaattaac aatataaaat tgcttattat
     2581 aagtgtatta ttgtatcaac aaataaagga attgtctcaa aattgatttt ttctaatcaa
     2641 atttttattt tgagacattc tcattttttt gcataagaaa atacccacca agctacggtt
     2701 tttcttaccg ctgttcggtg ggtattagta gtattatgaa atctgaaaac tttaagtatt
     2761 actacttccg ctaacgcaat cacaaaggtc gaaagcttca aagcgaaaat aggcttttta
     2821 ctcatcaaac gtcaccgtac ctgccaaccc gtccaccgtt atcatttgtc cgctcttaat
     2881 agttcgtgtg gcaatgtgtg tagccataac ggctgggatg ccatattcac gtgcgacaat
     2941 gcttgaatgg ctaagaggac caccaatgtc cgttacaatc gcacttgcag atgaaaataa
     3001 tggtgtccat gctggagttg tggtaactgc aaccaaaaca cttccaggtt gaaagctttc
     3061 aaaatctgct ggactattca gtacacaagc tggagccgtc acaacaccag tactggtacc
     3121 aatgcccgtc aacacaattt tcccatcttt ctgcttctgt ggtgcttgtg atatagtttt
     3181 tttgtttttc tctggcaact tggacggtga cacataaccc atgtattttt taagttcagc
     3241 ttttcgaaca ggtattatcc cgttcatatc agatagagac ttgtttttat ccaactgctc
     3301 tataagtacc tccaattccg attttgtgag ccaataaata tcatctaaat gtgaaatagc
     3361 tcctcctcgt atgaatcgtt ctgagatttc ctgaaacata caacgaatca gaggatgtcc
     3421 cattcccatc aaataaatag cattttcacg cataggagag gtttcctgtg cccaatggag
     3481 caacttaaaa aatagttttt tacgcaatcc acctatgtgt tgtaaaatct cttcttctgc
     3541 atgttttcta cgcttttcaa acgtggtctg gcgtaaaaaa ggactttccc cttttccttc
     3601 cacaaatgtt tttacggatt caaaagttgg tgtaagtgtt tcctgcggtg tggaatacgc
     3661 aaaatcaaac tcataagcgg tacgaccaaa ctctttaaag tacttattga ttcgattttt
     3721 ccattctatc catacttcct gtgaaactcc aacaggcata actgaagaca tgaaatcttc
     3781 tgctatcttt attgcaggac tgttttgcag ataaaggttg atgctgttat tttgctttac
     3841 ccattctgaa agaccccaca actccttttc cgattgcaga gcaatcgtat caaacccaag
     3901 caggaaaacg gaagtatccg ttataccagc acgtcgaact gctccttgaa ataattttgt
     3961 aaataatgtt tcactgatag aagcagcagg caatgtaagc tgaatccttg tgaaataaat
     4021 acatgcggca taaaatattg tctgaatacc atccataatt tgatgggcgt tcatcgtatg
     4081 caggggcttt tcttcccatt gtttaatagc atcctcatat ccttttcgtg ccgtttccca
     4141 gcgtgcaacg ctgttcataa gtgtcctacg taaagagcgt ggggaaaggg atttgatagc
     4201 aatcaaaagt ggctttgatt tagcagaaag ataaacatat ccattaataa ccgtatatgc
     4261 gccgtttttt ggtaataatt ttttcgcact tttaccaaac atatctatcc ataaaagctc
     4321 cgatgcacgg tttacgattt caagaccaag cgtggcgaac aaaggtgtaa caggatttgg
     4381 caagtgttct gccaaacttc cctttgtgta tacaacatcc tgttctggca atacccattc
     4441 cggtggcaga acagtaattg gacgagcttg aacaatgtac aatttatctt tttccagtgc
     4501 ccactctaca tccataggca tttgataata cttctcaatt ttctttccaa gctgtatcag
     4561 ttgcattacc tgattacgag taagagcata tttttttctc aacctttcag gaaccatggt
     4621 ttcttctgtt ccttctgaag tacgaaccgt cataatctct ttatttgcca cttcatacga
     4681 tataattcgt tcggagtcct tatccactac gattgtatcg ggtgtaacta agctagaaac
     4741 caccgcttca ccaagccccc atgcagcgtt aataatcatt tcgctacgtc taccattgat
     4801 tgggtttaac gtaaacataa taccagatga atcggaaaat gcaagctttt gtactacaac
     4861 agcaagtgcg acaatctcgt gctttatatt attcttcaca cgataagcaa tagcacgagc
     4921 tgtccataag gaagcccagc atctctttac agcgtcaata actttatctt caccttgtat
     4981 gttaagataa gtttcctgct gacctgcaaa agaagcgtca ggtaaatctt cagcagtcgc
     5041 agacgaacga acagccaccg atatgttccc taatccggca taagctgttt taattgcgtc
     5101 cgacacttcc tgtggcattt ctccattatg gaaaagctcc cctattttct tagatacatc
     5161 ttcaagttgg ttggtattat tggaatcaat accatctagc agtttattaa ttcgagactg
     5221 aatatggttc gtctcaacaa aaatctggta tgaagcagtc gtaacatgaa aaccgtctgg
     5281 cacagggatg cctgctgtca atagttttga taaagacatc ccttttccac caacaatttc
     5341 aatggttgct tgtttatgtt ccagtggcaa gacataataa atcataatca ttcacctcca
     5401 tcattcataa ttccgtgcag aaatatattc attgtttcat ccaacatttt ttccgaacta
     5461 tccgatggtc gtgacgtgta gctgattgat aacatggagt taattaacaa acgtgtcaca
     5521 agagcggtat ccgttttgcg aaagatgcct gtggtcacac cattatcaat taccgatttt
     5581 ataatatctt gatatgcata acgagcatct ttaagtctct ttcgatactc ttcttctaaa
     5641 taatcagatt ctgtatttaa ccacatcagt acagttctat actgctctgg aaatctaaga
     5701 tgattaagca tgatttttct aaggcactgt tgtggtgaag gttcaccatc aataatccta
     5761 tgaacttgtt ggatagtttt ttctatttct ttctcaactg catatacaac aatctcgtct
     5821 ttcgttttga aaaaatcgta tagacttgac tttcccatgt tggctaaaac tgcaatttct
     5881 ctcattgatg ttttttggaa tccattttca gccatgagac tcaaagcaac agcaactatt
     5941 ttttctcggc gttctgcttg ttgttcaggt gtaagcacaa ttccttttgg catagggcga
     6001 tacctccttt tagcgaccaa tggtcgtttc ttttttattt atcatacagc gaccatcggt
     6061 cacttgtcaa ggttaatttt tttcattgta tatggtactc gagataaaaa tgttcatttt
     6121 agaaattgct ctatttaagt agaaataggc ttcatcatta gaaaacaaga ctataaattt
     6181 gagatattta tcatgttcaa atttcatcaa tatcaaagtt taagatatta ataaaaaaag
     6241 aattactaag aaataacata taaagattca aaaaaggagt tgaacttaaa attcaattac
     6301 aaaaaatcaa atttaagaca actcctttat tctttatctc tactattctt tatctctatt
     6361 tatttattac aactccctaa catttaccac tactgcctta ggtctagtca tagcctcgat
     6421 tgaatactta acaccttgag ttcccatacc agatgccttt acacctaaaa atgggaagtg
     6481 gtctggacct ctttcagtct tattatttat ttgaacagta ccaacttcaa gtttatcagc
     6541 tatataaaat gccctatcaa tatcacttgt aaatactgat gattgaagcc catactctga
     6601 ttgattagca atttcaattg cttgatttat atctttaact cttataattg gaagtactgg
     6661 accaaatggt tcttcccaag caattttcat atcaacattt acattatcta acaatgttgg
     6721 atagaataaa ttattttttc taacattacc tgttattaac tttgctcctt tatgtaatgc
     6781 atcatcaacc aacccttgaa caaaatcagt tgctttatta tctataagag gagtgataac
     6841 tacttcatcc attggatttc caattgtcaa cttctctacc aatggcttta tttttccgac
     6901 taatttatcc gccacactct cttgtactaa aattctcttt actgccgtac atctttgtcc
     6961 tgaatatgaa tacgctccag atactatatt cttagctgca aaatctaagt ctgcatcttc
     7021 taatactata gccgcatctt ttccaccaag ctccaataat agtggtacca ttccagatat
     7081 tttagatata tgctgaccaa cttcagtact accagtaaag ttaataaaat taattccttt
     7141 atgcgttaca acataatccc ctatctcact acccctacct gtaatagtat taagtactcc
     7201 atctggtatc ccagcttttc tcatgatttc taccacatga agtgcgctaa tagccccttg
     7261 tgtagcaggt tttaatacaa ctgcatttcc accaataagc gctggagcaa tctttgacat
     7321 tgataaattt acaggataat taaaaggaga tatagctaaa acagtaccta atggaaccct
     7381 tgaaacataa gacatcttat ttcttgttcc accagggaaa ttatctccac taatggcttc
     7441 accctccata ttttttccca catcagctgt gtatcttaaa aaatctgctg ttctctcaac
     7501 ttctgccctt gcagatttta tgtctttggc tatttcaagt accagtatat tagctatctc
     7561 atctatattt tctaacaata aatcagcagc cttatgaaat atattggctc tcttgtatac
     7621 aggcatttca gcccatactt ttatactctc ttttgtgttt tttataacct catctacctc
     7681 atgttttgtc atagattgta ctttcccaac aagagaatta tctattggtg agtaaatttc
     7741 tattggcttt ctcgaattag attctaccca ttttccattt attaaatttt tatatacatt
     7801 ttcttttgtt ttaagttcat taaacatatt tttattcctc tctctgatat aactttgtgt
     7861 atttaaatta tagccattta ttcgttatgt aaacatttct ttttttcata aacgtttacc
     7921 tataaaaaaa attttaagtt aacgtttttc tagattcttc tattttaata acatactcgc
     7981 tccaaatcta tataggtcac actttaatat caatatacca atataccaat ataccaatat
     8041 aaatattata aacataaatc acttgacaaa tttataagta gacaatatta tatatctatg
     8101 acttaaaata tttttaaagt catagatagc atatgcagat agaaaggatt gataaaaatg
     8161 gctaaaagtt ttacagaaat agaaaaagac aatataagaa aaagactaat ttcagaatgt
     8221 gaaatcaact ggtctaaata tggatacaaa aaaactaaca ttgatgaact ttgttcaaaa
     8281 tcaggaatcg caaagggttc tttctataca ttttttgatt caaaagaaaa gttatttttt
     8341 gaagtactaa atggaatcca aaacagactt atatcatcag tagatgaaat tttaagtaac
     8401 atgcctccaa aagaaggctt tgcaaaatcc ttaaaaatgt tatatcgatt atatgataaa
     8461 aatccatttt tatattcacc aaataatcct gatttcatca gcctaataaa taaactacct
     8521 caagatatgt ttttacaact tgagtccaat aacatacaaa gtttttacga cttaattaag
     8581 agacacaatc tcaagttgaa gatagaaaaa gaaaaagcct ttggtgtttg taattctcta
     8641 ctatcaagtc tattggtaaa agaaaatatt gaatataact atattgaagt atttgatttt
     8701 atggtagata atcttgtaga acacatactt gaataacaat ttaaaataat ttttctaaat
     8761 atttctttat actaaagagg attaatatga ttgaattaat gaaaaaaata aacaatatca
     8821 tttacaaaat aaatgataaa ctaaatatac acacaaaact tatagacccc agagttaact
     8881 cattccactc tactctaagc catctataat agttcgtata tttctaataa tattagatat
     8941 taaagtaaga aaaaaattcc ttataataat tgctattata tcaattatta taaatatttt
     9001 aataatctta gataggtagg tatatatatg gtaaaatcct caaacacttt taataaaaac
     9061 ttcaacttga tgatattagg tcaaataatc tcattgtttg gtgcatcaat acttaaattt
     9121 gctttatctt tatacatact tgatataact ggtaaagcag aaatatttgc tactatacta
     9181 gcagtatctt caatcccaat tatagtattc tcccctattg gtggagctat tgcggatata
     9241 ttcaacagaa gaaacttaat ggttattttt gatttttcaa gcagtcttac agtactaatc
     9301 ttagcagtat tcttattcaa caataatggt tcaatattac ttgtaggaat tattatgacc
     9361 atattatcag ttataagcac aatgtatcaa ccaactgttc agtcaagtac acccctttta
     9421 gttgataatg agcatttaat gaatgcaaat ggtattgtag ctggagttgc atctcttaca
     9481 aatattgcag gacctgtatt aggaggtgtc ttgtatggtg tgattggaat taatgcaatt
     9541 atagttataa gttgtgtaag tttctttcta tctgcaatta tggagatatt tatacagatt
     9601 ccttttatca aacaagagca aaacagcaac attataaaaa ctatatttac tgatataaag
     9661 gatggtattg ttcatatatg taaagaaaat agatttatac taaaaatact ttttttagct
     9721 atagcaataa acttattttt aagctctatg ttaatagttg gtatccccta tatagtaaaa
     9781 atcactatgg gagctagtaa cttcttatat ggtctagcag aaggagcaat ttcttttagt
     9841 tcactggtag ctgctgttag tattggtatt ttctcaaaac atctaaaaat cactaatttg
     9901 cacatttcct ttattatatg tgcagttatg ttcataccaa tggcggtttc agtatatcca
     9961 tttatattaa acacaggatt tccagttcca tttttaatct ttatagtatc atctatgcta
    10021 atatttttta caacgactct tatctcaata tatgttataa cagtcacaca attaaaaact
    10081 ccaaatgaac tcttaggaaa agtaatgtca attctttttg caggaacagc aattgcaatg
    10141 cctcttggtc aggttatgta tggtaaactt tttgatttat tatcagatag agtgtacatc
    10201 attatactag gagttggatt tattacacta cttatagcat ttgtatcaaa gataacattg
    10261 aggaacgaaa atctttagat acaaagaatt tagatataaa gatttaattt tcactaattt
    10321 ctgaatttaa atgcaaaatt gcgtatcagt atgttgacgt tttagaaatt gattgatata
    10381 atcaatggaa aataaacttt ataaaaattc aaaatattca gctagaggag tgataaaaat
    10441 gagttacatg ccaactttag aacaagcatg ggaaatatta agaaaataca ataaggaaga
    10501 atttcatata agacatgctc aaattgtatc tggagttatg agatactatg caaaggagta
    10561 cgaccctgag agagtagact tttgggaaat agttggactt ttacatgatt tagattttga
    10621 aatgtatcct gatgaacatt gtgttaaaca aagagaatta atgacagagc ttgatttaga
    10681 taaatcaata atcgattcta caataagtca tggatatgga ttaactggtt cagatgttaa
    10741 accagagcaa tttatggaga aagtattatt tgctgtggat gaacttactg gtttaattgg
    10801 tgctgttgca ataatgcgtc cttcaaagag cgtttctgat ttagaattaa aatctgttaa
    10861 gaagaaattt aaagataaat catttgctgc tggctgttct cgagacgtta taagaagtgg
    10921 tgcagaaatg cttggatggg aacttgataa gcttatccaa acaactatag aagctatgag
    10981 aagcttgatt ccagagatgg caatatagct cctcatacat ataccataga agttacatct
    11041 ttaaagagca tttctctttt tgatgcaact tctttttata ttttgtagaa taaaaagttt
    11101 aatattttaa actcactata taatctataa taaatactat aaataatata atgtataata
    11161 gtcataaact ttctaacctt aaaagtttct aatactcaat aaacaattct aatatacaag
    11221 tggcattata tgatttaatt atatatcttc aatttatata ttaaaataaa tatatatatt
    11281 aaattcttat acaccaaatt tttacattaa acttctatgc accacattta tacatattaa
    11341 attcttatat attaaatttc tatgcatcac ttttatacat attaaattcc tatatattaa
    11401 acttttatgc gtcactttta tacatattaa attcctatat attaaacttt tatgcatcac
    11461 ttttatacat attaaattct tatatattaa acttttatgc atcactttta tacattaatt
    11521 cttatatatt aaactt
//
LOCUS       DAELXX010000114        11452 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.115, whole genome shotgun sequence.
ACCESSION   DAELXX010000114 DAELXX010000000
VERSION     DAELXX010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11452
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.115"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            87..872
                     /locus_tag="KPH29_003037"
     CDS             87..872
                     /locus_tag="KPH29_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBG1468612.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKQSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPMSIDTMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKEYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            1151..3061
                     /gene="phnW"
                     /locus_tag="KPH29_003038"
     CDS             1151..3061
                     /gene="phnW"
                     /locus_tag="KPH29_003038"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBG1468613.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNLWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     EYTTPIPHVVETIEKLRKNGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLNIEFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEKLSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            3418..4239
                     /locus_tag="KPH29_003039"
     CDS             3418..4239
                     /locus_tag="KPH29_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468614.1"
                     /translation="MNEELSSLRFDGVGKCKGGIFDNVNISGTGKIEGSVKCSKFDSS
                     GSAKVFGTTECNEFFTSGLSKVEGNILASKVEISGLLRCTGNINSVEIDTSGIINVEG
                     SIKSDIIHGEGYLKANENIDCENINLSGILECNGFLNCEEVNISLNGTSNFNEVGASS
                     INIKKGHGDYCKKFRMLEKKHINFEKVSSNLISLGLLSIRKYTESKLFANIIEADEIS
                     LENSEVKVVRGKNIKIGKGCYVESLEYSESIEIDEDSIVGEIKNISEKIKLKKDK"
     gene            4280..5047
                     /locus_tag="KPH29_003040"
     CDS             4280..5047
                     /locus_tag="KPH29_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HBG1468615.1"
                     /translation="MEKGSIKILGEGFISSGEYENIKIVGSSKSKGNVKSNEVKVLGD
                     AKFEGDINIKNFVVNGKTIIRGNLKAERVRVNGQLNIEGDAEIDDLVITAQQKVDGKL
                     KCNNVTIRGQLSVNENMYAQDIKIYGEMRNKSNIECEDIKVYGVIKCDGLLNGENIYV
                     YSGGGSYCKEIGATNIKIGRSKDAFSLIKIINIFSGKFKCNLIEGDTIELEDASIKDI
                     RGKSISLVDNCDVGNIEYSDNLSVSDNSTVKNSIKVG"
     gene            5055..5675
                     /locus_tag="KPH29_003041"
     CDS             5055..5675
                     /locus_tag="KPH29_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbD family protein"
                     /protein_id="HBG1468616.1"
                     /translation="MEELISKKDLLSKTNISYGQLYRWKRKNIIPEEWFIKKSAFTGQ
                     ETFFPRDKILERIELILSMKEETSLDDIANMFMKKDINKEFDIDFILSKEVICNYTKE
                     IFQNLYKKEQSIGKKELLILSIIEKFLVKSIITFEELKLIVNIIEENFTSIYNESGKI
                     YLFRKFGVPFVVGCLDYKKVKFEKDVVKIIEIDLTKEINDISIKLS"
     gene            complement(5975..6562)
                     /locus_tag="KPH29_003042"
     CDS             complement(5975..6562)
                     /locus_tag="KPH29_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBG1468617.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(6648..7085)
                     /locus_tag="KPH29_003043"
     CDS             complement(6648..7085)
                     /locus_tag="KPH29_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG1468618.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            7189..8187
                     /locus_tag="KPH29_003044"
     CDS             7189..8187
                     /locus_tag="KPH29_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HBG1468619.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMTGKHVGRTVVDLEKI"
     gene            complement(8307..9233)
                     /locus_tag="KPH29_003045"
     CDS             complement(8307..9233)
                     /locus_tag="KPH29_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBG1468620.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTVLYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNMYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            9619..11223
                     /locus_tag="KPH29_003046"
     CDS             9619..11223
                     /locus_tag="KPH29_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG1468621.1"
                     /translation="MKEKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGAANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVLDNNREFIKKYINR
                     FKVGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENNIRPVMIHAQTVRADQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
BASE COUNT         4515 a         1227 c         1955 g         3755 t
ORIGIN      
        1 tttaatacat aaatatgatt tcataaatct taggtatcat tgtaatatta taggagaaaa
       61 atatattata tttaggagga aaatgaatgt ctttaaattc gatagataga aaaaaagaaa
      121 tacttaaatt attagattca aaagggaagg taaatactaa agaattagta aaaagattag
      181 aagtatcatc agaaacaata agaagatatt tggaagaatt agaaaatgaa aataaattaa
      241 aaaaggtata tggtggtgct ataaaaataa attatggagt gattgaacca gaacatataa
      301 agagaaacaa tactaaccta gataaaaaac aaagtattgc taaatacgca gctaagtttg
      361 taaatgacaa tgagtcaatt atcatagatg aaggtactac caatttgcag attgtagatt
      421 atataatatc tgtaaacaat ttaaaaatta ttacaaactc ttatccagtt ctatcaaaat
      481 tgatttctta tgagaatcaa ggtttgtttg atggagaagt tatatttatt ggtggaaaag
      541 taaatttaaa acaccaaagg acagcatgtc caatgtcaat agacactatg aaaaacttat
      601 acgtagataa agcttttata tctagtgaag gtatacttga tagttttgga attagtagta
      661 tagaccaaaa taaggcattg ttgtcaaaag aatatataaa aaattcaaag gaaagttttg
      721 tattatgtga tagttctaaa ataggaattg ctagtatgta taagatagca gatttagagg
      781 aattagattt tgttatttgt gatgtagaac ctccgaatga atggaaggat aagttaaatg
      841 agataaatat aacatggata gaagcaaaat aaacccttag ttttatataa tacaactatg
      901 cttaatacaa ctaaattcat aaaatatagt cagttcatag attaagatta gaaagtaaat
      961 tttagattgt aacttaggat tttatggaaa tatgatataa aataaaaaca attaaataca
     1021 actaaaaaca gaaatttaat atatatttaa cctaaatatg atgtataatt aacatatgaa
     1081 agttatactt taaaagtaaa aacaattaaa aacacaaaaa ttctaaaatg aatctatgga
     1141 ggctataaat atgaaaaaga tatatggaga aaaaataaaa gcagtagtat tcgattgggc
     1201 tggaacaaca gtagattatg gatgttttgc acctcttaat gtatttatag agatatttaa
     1261 aagaagaggt atagatgtaa caatggaaga agcaagaaaa cctatgggaa aattaaaaat
     1321 agaccatata agagaaatgt gtgaaatgga tagaataaaa aatctttggt cagataagtt
     1381 tggtaaagtg ccaactgaag atgatgtaaa tgaactttat gctgaatttg agccaatgtt
     1441 atttgagaca ttagaagagt atacgactcc aattcctcat gttgttgaaa ctattgaaaa
     1501 gttgagaaaa aatggattaa aaataggttc tacaacgggt tatacaagag agatgatgaa
     1561 catagttgaa ccaaatgcag caaagaaagg atactcaccc gattttcttg taacaccatc
     1621 agaagtatct caaggaagac cgtatccttg gatgtgctat aaaaatgctg aagctttagg
     1681 ggtgtctcct atgagttcta tggtaaaagt aggagataca ataagtgatg taaaagaagg
     1741 cgtaaatgca ggaatgtgga gtgttgctgt aataaaaggt tcaagtgagt tagggcttac
     1801 acaagaagaa gtagagaaca tggataagga agaattaaaa gctaagatga gtatagtttc
     1861 aaagaaattt aaagaggcag gtgcacattt tgtaatagaa actatggctg agttagaaga
     1921 tatactaata aaaatagaga atgaaactat taaatcagat tttgtaccag aaaatgatta
     1981 catattactt acaccaggac cactatctac tactaaatca gtaagagcat ctatgttaaa
     2041 agattggtgt acttgggatg ttgaatataa taacctagta caagatgtta gaagaagatt
     2101 agtatctcta gcaacccaaa atacagataa atacacaagt gtattaatgc aaggaagtgg
     2161 gactttttca gtagaagcta taataggaag tactatttca aaagatggta aacttcttgt
     2221 aattgcaaat ggagcatatg gaaagagaat gaaagatata tgtaattatc ttaacataga
     2281 atttgttgac tgcacattta aagatataga agcagtggat ttaaatgtag tagaaaattt
     2341 attaaaagaa aacaaggata taacacatat atcaatggtt cactgtgaaa ctactacagg
     2401 tagattaaat cctatacaag aggtagggaa acttgcaaaa gaatataaca aaatttatat
     2461 agttgatgct atgagtagtt ttgggggaat tgagattgat gttgaagatt ttaatataga
     2521 ttttttagtt agtagttcta acaaatgtat acaaggagta cctggatttg gttttataat
     2581 agctaataaa gagaaattat ctaaatgtaa aggtatagct aaatcattat ctcttgatgt
     2641 gtatgcgcaa tgggagacta tggagaaaaa taatggtaaa tggagattta cttcacctac
     2701 acatgttgta agagcttttt atcaagcttt attagaattg gaagaagaag gttctgttga
     2761 aaaaagatat gctagatata aagaaaatca gtttacaata gcttcaagat taaaatcttt
     2821 aggatttgat actttggtga atgataatgc tcaatcacct gtaataacaa ctttcttata
     2881 tcctaaaaat gcaaagtttg aatttatgga attttatact tatttaaaag ataatggatt
     2941 tgttatctat ccaggtaaat tgactgatat agatacattt agaataggta gcataggtga
     3001 agtttatcca actgatatgg agagactagc tgatgtaata gaaaaattta tcaatagata
     3061 gaagaagata gtgtctttta ggttacagaa tagtgcggag agcctactcc tttaggtgtg
     3121 aaatgatagc acaaacagtg tataaaaagt attgacagta gaatttaaag acttatattt
     3181 tagaagttta tacaatgaat atataaagat tgagtaatag ggatatattt tatttaaacc
     3241 acaatctaag aaattagatt tctagtttaa ataaagcatt tccctattat ttttatgcct
     3301 atttatagaa aataaatgat agttgcttta ttaagtatta ttatcagact tgacaacgta
     3361 acaatgtttt gttatattag gtttaaagat aaaatacggt aaagaaagga tatcattatg
     3421 aatgaagaat taagttcact aagatttgat ggtgttggaa aatgtaaagg tggaattttt
     3481 gataatgtta atatatcagg aacaggcaaa atagaaggaa gtgtaaaatg tagtaaattt
     3541 gattcttctg gttcagcaaa agtttttggg acaacagaat gtaatgaatt ttttacatct
     3601 ggtttaagta aagtagaagg aaacatactg gcatcaaaag ttgagatatc gggacttctt
     3661 agatgtacag gaaatattaa ttctgttgaa attgatacta gtggaattat taatgtagaa
     3721 ggaagtataa aatcggatat tattcatgga gaaggatatt taaaagcaaa tgagaatata
     3781 gattgtgaaa atatcaattt aagtggaatc ttggagtgta atggattttt gaattgtgaa
     3841 gaagttaata ttagtttaaa tggaacttct aattttaatg aagttggagc aagtagtata
     3901 aatataaaaa aggggcatgg agattattgc aaaaaatttc gtatgctaga aaaaaagcat
     3961 atcaactttg aaaaagtgtc ttcaaattta ataagtttag gtctattatc aattagaaag
     4021 tatacagaaa gtaaattgtt tgcaaatatt atagaagcag atgaaataag cttagaaaat
     4081 agtgaagtca aagttgtaag aggaaaaaat attaaaatag gcaagggatg ttatgtagaa
     4141 agtttggagt acagtgaaag tatagagatt gatgaagatt caatagttgg tgaaataaag
     4201 aatataagtg aaaaaataaa attaaagaag gataagtaat aaataggttt aaaaataaaa
     4261 tttaaaatga ggtaataata tggaaaaagg gtctataaaa atacttggag aaggctttat
     4321 aagtagtggt gagtatgaaa atataaaaat cgtgggaagt tctaaatcaa aaggaaatgt
     4381 taaaagtaac gaagtaaaag tccttggaga tgcaaaattt gaaggtgaca tcaatataaa
     4441 aaattttgtt gttaacggaa aaactattat aagaggcaat cttaaagcag agcgtgtaag
     4501 agtgaatggt caacttaata tagagggaga tgcagaaata gatgacttag ttataactgc
     4561 tcaacagaaa gtggatggca aacttaaatg taataatgtt acaataagag gacaattaag
     4621 tgtaaatgaa aatatgtatg cacaagatat aaaaatctat ggcgaaatga gaaataaatc
     4681 aaatatagaa tgtgaagata taaaggtata tggagtaata aaatgtgatg gtcttttaaa
     4741 tggagaaaat atatatgttt attcaggagg aggttcttat tgtaaagaga taggggcaac
     4801 taatataaaa ataggaagaa gcaaagatgc tttttcatta attaaaatca ttaatatttt
     4861 ttcaggaaag tttaaatgta atttgataga gggagatact attgaattag aagatgcatc
     4921 aataaaagat atacgaggca aaagcatatc tctagtagat aattgtgacg ttggaaatat
     4981 tgaatacagt gataatttat cagtttcaga taattctact gtaaaaaaca gtataaaggt
     5041 gggctagaaa agttatggaa gaattaatat ctaagaaaga tttattgagc aaaactaata
     5101 tatcttatgg tcaattatat agatggaaaa gaaaaaacat aataccagag gaatggttta
     5161 taaaaaaatc agcttttaca ggtcaagaga ctttctttcc aagagataaa atactagaaa
     5221 gaatagagtt aatattgtct atgaaagaag agacttctct tgatgatatt gcaaatatgt
     5281 ttatgaaaaa ggatattaat aaggaatttg acattgattt tattctcagt aaagaagtta
     5341 tatgtaatta taccaaagaa atatttcaaa atttatataa aaaagagcaa agcataggta
     5401 aaaaagaact gcttatattg agcataatag agaagttttt agttaaatct ataataactt
     5461 ttgaagagtt aaaattgata gtgaatataa tagaagaaaa ttttacatca atatacaatg
     5521 aaagtggaaa gatttatttg tttagaaagt ttggagttcc attcgtagta ggatgtctgg
     5581 attataaaaa agtaaagttt gaaaaagatg tagttaagat tattgagatt gacttgacaa
     5641 aagaaataaa tgatataagt ataaagttat catagtgtat tatcttatat aatgtattat
     5701 aataatctac ttaaaataca ctttatttaa atatttagtt taaagataag attttatgtg
     5761 agtatgtaaa agttaaagat agatttaagt aggttatagg gattagtaaa taataaaatg
     5821 aaccatgatg aattactata aattgtattt aaactacatt ttctacttat agtaatatca
     5881 atggttcatt ttattgttat taagataata cagtatttat aaaaatactt gatataaata
     5941 tagtaaattg tttaaataga attattgcct tagtttattc aaataatgag tttttaaaat
     6001 ctaagaaata tccttgtgga tgaagacata caggacaagc ttttggagct tctttaccta
     6061 tatgtatatg gccacaatgt gtacatatcc attgtatatc ttcatttctt ctgaataaag
     6121 taccttcttc taaatcagta gcatatctac caaatctatc accatgaact ttttctattt
     6181 cagctatatt ttcaaataac ttagctatag attcaaatcc ttcttcacgt gctgtttttc
     6241 caaagttttt atatacatca tcccactctt gaagctcatt atgttgtgca gctttaaggt
     6301 gttgtgctgt cttgtcatat aaatctacag ggtatgctcc tgatatactt atattttcac
     6361 cagcaaattc ttttaattta gaaaagaatt gttttgcatg tgatttttct tggtcagcag
     6421 tgtaattaaa taaagactct attatatata atccttcttt ttttgctaca gatgcagaaa
     6481 tattgtatct atttctagct tgactctcac ctgcaaaagc tcttaataaa ttttctttag
     6541 ttttactatc ttttaaattc atattctacc tctctatcat aataaatttt tacaatattt
     6601 gattcttttt atttattatt ccaataggag atataattat gctcaattta gtcagcattt
     6661 ttaaaattat cattaatttt ttttaaaatt ctaagaaatt cttgtttttc attttctgta
     6721 atattatggt atgctgtctc tttaacttgt gttgagatgc tattaaaagt atcttctata
     6781 agcagtgcct tttttgtaag ctttatatac gtgatacgtt tgtcatttgt acatttttgt
     6841 ctttctaaat atcctaggtt tataagttta ttaaccaaca ctgtaacagt ggatttatct
     6901 tttcctattt tagctgcaat ttctttcatt gtaagtattc cattattttc ataaagtgca
     6961 gttaaaatat ttccatgagt aggtattaac tcacctaaac catttttact gagtttattt
     7021 tcaatgaatt taatcatact agcctttgtt ttacttatga aatgtacaat atatttatct
     7081 tccataaatt attctccaat ctttaatcta tttaaattag tttgacataa aactatttat
     7141 aaactataat tagtttaaca taaaactaaa tgtattggga ggaaatttat gaataaaaaa
     7201 tttaaatcac tatatattac agaagaagaa ggaaatttta gaagaaatat agttgaaaga
     7261 gaagtagata cattgccaga aggcgaagtt ctaataaaag taaaatattc ttctctaaat
     7321 tacaaggatg cactttctgc aaatggtaat aagggtgtta cgagaaagta tcctcataca
     7381 ccaggaattg atgcatctgg tatagtagaa tctagtagtt gtgatgattt taaaattgga
     7441 gaagaagttt tagtaacagg ttatgacttg ggaatgaata cttctggtgg tttttcagaa
     7501 tacataagag ttccttctag ttgggtagtt aaacttccta aaaatctttc actaaaagaa
     7561 gcaatggatt atggtacagc agggtttaca gcagctttat ctgtgtataa atttatagat
     7621 tctgtagaca agaatatggg agatgtatta gtcactggtg gtactggagg agttgctgta
     7681 attgcaacta aaatattgat taaattaggt tattcagttg tagtttcaac aggaaaatta
     7741 gaagaacaaa aagaagtatt actaaactta ggtgttaaag atgttataca taggtcagaa
     7801 gtggatgata actcaggaag acctctgctt agaccaagat gggctggagt aatagataca
     7861 gtaggaggaa atactctagc aacagcaata aagacaacta attattgtgg agcagtaaca
     7921 acttgtggta atgcaggagg agttgatttt acaagttcag tatatccatt tatcttaaag
     7981 ggaattactt tatatggtat agattctgtg gattgcccaa tgaacttaag attaaaaata
     8041 tgggatttat tatcaaatga atggaaatta gatgatttag atgatatggt aaatgtagta
     8101 tcattggaag agttaaatga tagtatagat tcgataatga caggaaaaca tgttggaaga
     8161 acagtagttg atttagaaaa aatataggcg aataaattta taattaggat tataggtttt
     8221 aagcaaaaat gagatacttg aaatttactt atgagtatct cattttttta tgtaaaaaaa
     8281 tttatgtaga tgtttgtgta aaaagattat tcatgaacaa ttaattcaaa tacatctggt
     8341 cttgaatagt gaccttctga atcaaaatct agtctactaa gtactatttt ttctaggtct
     8401 aaatcagcta ttagcatttc ttctttattg aaaataggac ctgctacata ttttccaaaa
     8461 gggtcaacta tacaacttcc tcctggacac ataatttctg gttctgcatc tagctcctta
     8521 tagtagttaa ggtcagttgg atacatattt ttttcaacat attgattgca tccaattaca
     8581 aaacatcttc cttctaaagc aatatgcttc atagttgctt gccactcttc tcttgaatct
     8641 gcagtagggg ctatgtaaag ttttacaccc ttagcataaa gaactgttct agctaatggc
     8701 atataatttt cccaacaaat caacgaaccc atttttccat atggagtatc aactactgta
     8761 agagtacttc catctccttc tccccaaata catctttctg ttcctgtagg tttaagtttt
     8821 ctgtgtttac caattagctt accttctgga gagaaaaata agtttgtgca ataaagggta
     8881 caatttatat cattaccatc tctttctgtt atacccattg atagatatac tcctgcttct
     8941 tgtgctgctt ttcctaataa atctgttgta gcactaggaa ctggtacaga attatcatag
     9001 tatcttttcc aatcttctct accttccatt gttctacttc caacaacaaa tccaaatgaa
     9061 agacctcttg gatatgcagg tatgaatgct tcagggaaaa ctacgatatt tgcccctttt
     9121 ctaccagctt ctttaattaa tcctaatgct ttttcaacag ttttttctaa atccattatg
     9181 actggtgacg cctggacaac agcaattctt acatttttat tttccttgct cataaaaaac
     9241 ctcctaaaaa atatttaaat tttttgcatt gaatagatta catgagatgt tatttgagaa
     9301 ctaagatact tatattgtga ataataatgt aactgtatat ttaaaataat acatcatgat
     9361 ttaaaaaatt acaattgtac agatataatt gagtttagat atttaaattt aacaacttat
     9421 aatatttttt caattagtat aaagcctatt aatttgtctt atttacaatg aatagtattt
     9481 acttcttggc aaacaataat taaaaataaa acttttatat gattaaactc taaagaaaaa
     9541 gtttataaag aagataatat aattaagcag aaaataatga aaaaaattta ttaaagtaga
     9601 caacgtaagg agaattttat gaaagaaaaa atttattaca atggaaatat catcacaatg
     9661 gaagattcaa tatgtggaga tgctattctt ataaaagata aaataataaa aaagatagga
     9721 acaaaagaag aagtatttgc tttgaaaaat aaagatacag aaattataga tttacaaggt
     9781 aagacattga tgccatcttt tatagattca catagtcact tgatagcttt tgctactact
     9841 ctaaagctag ttcctttaga agatgcaacc agttttaaag acatagtaaa aaaaatacag
     9901 gatttcaaag agtcgaataa tataaaaaaa ggagactgga taattggatt ttcatatgat
     9961 aataattttt tagaagaaaa taagcatcca gataagagtg tactcgatag tgcatcttca
    10021 gaaaatccaa tactaatttc acatgcttct ggtcatatgg gagctgcaaa tacattgggg
    10081 ttaaaacagc ttggtgttac caatgaaaca cgtgacccag aaggtggaca tataggtaga
    10141 gtagaaggca gtgaagaacc aaatggatat ttagaagaaa atgcattttt taatgtagca
    10201 tctaaaataa aacaaccatc atcaaatgag atttttaatt caattgaaaa agcacagaac
    10261 atatatctaa gctatggtat tacaacagct caagaaggtt taatggaaga aaatcaattt
    10321 aacattttaa aagcaatggc aaatcaaaat aaattaaaaa tggatgttgt aggatatgta
    10381 aatttgaaaa aaagtaagtc tgtattagat aataataggg agtttataaa aaaatatata
    10441 aatagattta aagtaggagg ttataagatt ttcttagatg gttctccaca aggtaagaca
    10501 gcatggttga gtagaccata tgaaaattca gatgatggtt attgtggata tcctatatac
    10561 aaagatgaag aagtagaaaa gtttatagat atttcactaa aagaaaagat gcaactctta
    10621 actcattgta atggagatgc agcagcagac cagttgatag atgcgtttga gaaagtttta
    10681 aacttaaaag agcaaagtag tgaaaataat ataagaccag ttatgataca tgctcaaaca
    10741 gtaagagcag accagattga tgatatgaaa gtaataaata tgataccttc atactttgta
    10801 gctcacacat attattgggg tgatattcat atcaaaaacc ttggagaaga tagagcgttt
    10861 aagattagcc cacttaaaac aactatagaa aagggattaa tttacactct tcatcaggat
    10921 acaccagtaa tagcacctaa tatgctggaa acagtatggt gtgctgtaaa tagaattaca
    10981 aaaaaaggta tacaaatagg agagaatgaa aagataagcc cattagatgc tttaaagggt
    11041 gttactataa atgcagcata tcaatatttt gaagaagata aaaaaggttc aattaaagaa
    11101 ggtaaattag ctaatttgat aatattagat gaaaacccac ttacaatcga ccctatgaaa
    11161 ataaaagata ttaaagtatt gcagactata agagaaggag aagtattgta ttctttgaag
    11221 tagatgaaag ttagatattt tataatctat aaaaaatagt tttatataat aagttaggta
    11281 gaattaaata agattacagt taaatcaaca attatatttt ttattaaatt ttaattattg
    11341 tattgaagat ttataagtat aattttattg attctacctt tttaaataac tgtcgattta
    11401 ttaaagttat ctatgtacta tattaaacaa ttaagtagtt attaattgca ct
//
LOCUS       DAELXX010000115        11354 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.116, whole genome shotgun sequence.
ACCESSION   DAELXX010000115 DAELXX010000000
VERSION     DAELXX010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11354
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.116"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            124..543
                     /locus_tag="KPH29_003047"
     CDS             124..543
                     /locus_tag="KPH29_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468622.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSPINLKSGDIFTFIRLTLLAGRMF"
     gene            568..5625
                     /locus_tag="KPH29_003048"
     CDS             568..5625
                     /locus_tag="KPH29_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HBG1468623.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISNYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVTSALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFDARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNSDRQELYNKLILLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSNSSSKLNKIISNISLSTSEDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKFKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSHLANNIQYFEGIDYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDESGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQLVQPFKQVFREYYRINSDEISEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNINFEGSHV
                     LITGNLGEYSVHMGSGIVHKKGMGALNILPVHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDNKIKDPSILSNL"
     gene            complement(5785..6522)
                     /locus_tag="KPH29_003049"
     CDS             complement(5785..6522)
                     /locus_tag="KPH29_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1468624.1"
                     /translation="MIKVLICDDDKIVRDDIANIVKESGYVDYVKKVKDGIEAIEFIK
                     SEKIDLLIIDIDMPYKNGIDSAKEILSIDKDIHIVFVTGFADYSLESFKVHPIDFIVK
                     PFKKEKILDSINIAIDHINSHKIAKNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLYTRDEDTIKFYENFDDLKKRLPPNFFMTHRQYIVNLRLIHKVIPINKSSLEIKF
                     INFQESATLNKSLEKDFLYRFYRVKRF"
     gene            complement(6541..7476)
                     /locus_tag="KPH29_003050"
     CDS             complement(6541..7476)
                     /locus_tag="KPH29_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG1468625.1"
                     /translation="MENNFIFPIVILTLFIFSNLIMNKYRFYKELDILKRGILTFFVS
                     CFVWILFMEGIEYIQLSLGYNFLLEGVLNIILFLILIILSFYIGFKFIRLLDNNNKQE
                     YKLQKLDSKINHKDNIIVKLRSQKHDYLKHLQVVYSLLNTGLAEDAKEYITNISNSFK
                     SKNSRYGKVSYLDAIASLKYEECLEKNISFDVYIQDFMDNLEIEPNDMSSVLLNIIDN
                     AIESLSAIQKEKKYIRLHAYEDEFQYIIAIKNNGPKIHNVESIFLDGFSTKEGQDRGF
                     GLYLVKNILDKYDYSILVNSDDFLTEFVILIPKYY"
     gene            7684..10269
                     /locus_tag="KPH29_003051"
     CDS             7684..10269
                     /locus_tag="KPH29_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG1468626.1"
                     /translation="MKDNLKIALRYIISYKARSLAIALSIILATSLIVGIGTLSRSAQ
                     QAEVDKLKRETGSDHVYFKDINKNQLEYIKSRKDIKNLGITSHYGYTDPNERLAINLE
                     YANKNYLTNQSKLIKGHLPKASNEVVVEKWILNSLGLKPEINQNITFKLYQKEKPETF
                     KVVGILEDRPIEKNKGTCEIFLNLNESKLDKFSYAYIEFNNGIDINTSIDNIVKNTML
                     DENSVGKNKMLIESTRENGTLDNSSKYTAITMSLFSGIVIYSIYVISIYQRIQEYGIL
                     RAIGSTNFKIFKFMFYELFILALIAIPIGICTGIGGAQIFNRTAGNIQFEGNVTVTPF
                     VIPDKIILLSIGSIILTILIISFFTYLKIRRISPIDSIRKTFGTDKNINKVNSLISKL
                     TLNISVTKSISAKNIFRNKKGFIIIILSMSLGGIMIIKENYKYSFSDIQNKNGQEETY
                     MNADFILTNFFLKLNQSNKADSFKDIKGLNDNQIDKIKNINGIDKVKTASILDTRIEV
                     DKLNGLDYYNIINSTPYYKDFPLFIKNKNTGKYTMKQKLRGYNDEMINSLEKYLVSGS
                     INLERMKKENLAILYVPQVSKTNKYKLSYTPGTETPAVDIKVGDTIKVKYPKGEIDQD
                     LYIKLKDNYEYLEYEFKVGAIVSYPFADNYLYSGDQGIDVITSNDYLKKLTGVDKYNV
                     VYVDINKNANVKKINTLLGEIGSESPGTTTANMLMEKENFDKMTARALTYAYGIVAVM
                     FIISVFNIINNISYNLTSRTSEFGMLRAIGISERGFKNMILYEGILYGILSSVITIVV
                     GLIIQFRMYYTYNFVSYGLGFSIDYKIYILVVLANIIVGILATYIPLRKINKISIVEA
                     INITE"
     gene            10281..10952
                     /locus_tag="KPH29_003052"
     CDS             10281..10952
                     /locus_tag="KPH29_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1468627.1"
                     /translation="MPILEILNLGKIYGKKETAVHALKDANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSSGTIKVDGKDICCLSDKKLAKYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKVDEEYIEDIINLLGLSERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            10979..11320
                     /locus_tag="KPH29_003053"
     CDS             10979..11320
                     /locus_tag="KPH29_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468628.1"
                     /translation="MLLILDNLNLNLDFDYRIIREENDDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRINKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIINIKNQN
                     EKVILNKSIKK"
BASE COUNT         4419 a         1470 c         1665 g         3800 t
ORIGIN      
        1 tttttctatt ttttgtaaaa taattccata attcacatta ataattcaca aaatgatata
       61 atataacaaa tagataaatt ttaaatttct aaatacataa ttaatatttt gcgaggtgat
      121 tttatgcaaa tttattttag agttaaggct gttggcaaac gcaaacctat gttagaactt
      181 accccttttg atgtatctga caacatcaat tgcctaaaag aagctatctc tgaaattgta
      241 tctaaaaatg ttcttgaata taatgagaaa atcccagaaa aatcaataat aaacttctta
      301 actaatgaag aaattgaatc tcaagctgat attggaaaag ttggatttgg ctctatttac
      361 aatgagaata aacaatctgt agaaaaggca atagaagtag cgttacaggc atttgaagat
      421 ggaatctaca aagtgcttat aaatgaagat gttattgaaa aactcgattc ccctataaat
      481 ctgaaaagtg gagatatatt tacttttata agacttacct tgcttgctgg aagaatgttt
      541 taaaataaat aatttggagg tacataaatg ttagaacgta ctagagtaga aaaaaatcct
      601 aaaataattg aaaattttaa aaataatttt actaaatctt cttcaaaaca gtactacgac
      661 ttagcaaaat ctctttggga ctgcatagaa aatagttcat actattatag agaacctgag
      721 gacaaattct taaatatgat aaagaaatac tacaattgcc actactacga aattaaaaat
      781 atggacgagc tcctttctgg acctttaaaa aatgcattag aattaatatt tacttctgaa
      841 caagtagaag aactttgcca attaattaaa agaaagatgg attatacata ttcaggagga
      901 tattatcgta aagggtatcg ctcaaataaa cttgtggatt attttagagg tattataagg
      961 tttatttcaa actatgttga tttaatttat tatgattttt cagttataga ttatcttact
     1021 cttccaaata aggtcttgta ctatgactct ataatgagtg ataaaatagc actagaaata
     1081 gacaaaggaa atcaaaaagt aacaagtgcc ttagaagaag taatatttgg agaaaatcaa
     1141 actgctctac ttacaagaca aatgataatt ggtatcatga agagtcatag tgataaatgc
     1201 tatgaaatgc tttctaaact tcttttggca gccaaacaac aagaaggact tagacagcaa
     1261 attgtagaga gtttagatga gggtacaatt tctgcatcaa tatacatgtt aaaaactatt
     1321 ttggacaaca atttagagcg atttagttct gttataagag ccttggatac atggacagga
     1381 cttggttttt cagaccaaaa acctggaata atacgtaaat gtttagaact ttcatataaa
     1441 tcattgactg atgaaaagtt ccgtatagaa tcaattaata gcaatgataa tcttgaaatt
     1501 tattttgcct tatgggctac tgcaacttac aacatatatg actcttactc tcttattgaa
     1561 aacttacttt cttgtcagga aaaatataaa aaacttgtag ctctttactt tttatcgcat
     1621 acagaaagta catattttaa acattctata gcatctaaaa acattaaggg tgatgattta
     1681 gagatactag catgggtaat tacaaatttc tatgtagaag gttctgttgg ctactactat
     1741 agttggcaaa acagagaaac taattttgac ttgtcaaaca ctcctattga caaagacttt
     1801 gatgctagaa aagaacaatt tgaaagttta aaacatactt tagaaatttt aaattcaaag
     1861 acaaaatcat tttcaggaag tgtatttcca tggacttgga ttgaaatttc ttctcatgaa
     1921 atacttaaat gtatgatgac tttaacagca tacgatatga atgctcaatt tatagatgtg
     1981 cttatggaaa attgtcatct tatgagttca gatttaagag cttcacttat agtcaatttt
     2041 gtaaatgaac caaagacaga tagacaacgt gaatttttat tggaatgttt aagtgataag
     2101 agtgtttcca atagagaaac ggtattgagt ttacttagaa agcttgagct aacagatgta
     2161 gaattagata agataaccaa tctattgtct ttaaagacaa gctctattag acaaacagta
     2221 ataaaaattt tacttgaaca agatgattca aatttggaaa atattgcttc ttcactgata
     2281 aaatcttcaa attctcaaaa atgccttgga ggtcttgaaa ttgtaaactt attaaaagac
     2341 aattctgata gacaagaact atataataag cttattttac ttatagagga tattgataaa
     2401 tctaaaaaga atacatctca agttcaaata ttaattgata atttgttagg taaagcaaaa
     2461 aatgattact gcaaagaaaa tggttttgga atatataacc ctgaaaacaa agttcaaatt
     2521 ccagaacaat ttgattttaa agatgattct aatagctcat caaagcttaa taaaattatt
     2581 tcaaatataa gtttatctac tagtgaggat tctgtttctg tagcaacaaa aaaagaagca
     2641 aaaaaaattc tcgatacgga tataaataaa ataataaaag tttatgaaaa atttgatgag
     2701 cttattactg aaaatgcaga ttatgaatat gaagttgaaa gatggtcagg taatgtagac
     2761 aaagtgcttt taggttattc tacttataac ttgactccta ttaaatataa agtttctaat
     2821 gatactcttg ataattatcc attggcagaa ctttggagag aaactagtaa aaagcttgac
     2881 ttagattaca aattaatact tgatatctta ttttatagta taagtacatg tggatataat
     2941 gaaacagaaa aaagttggtt ttcatctata tattcaaatg tccttgactc taaaaaacaa
     3001 aataaaatat atacaaaact tgaaaaattt aaatttaatg gacttacaag aaaactttta
     3061 agagttttac taaatgagtt tgaccataaa gaaatattta actattgttt agaaatatac
     3121 tataaactta gaaacttatt gactatagaa caatgtaaaa aacaaagagt tgaatttact
     3181 aaaaactatt acagacaaaa tgaatactgc cctgctttaa atgcccctat tttaaactat
     3241 tggcttgata aagcacattc gtttatttat gatgatattt cttttactaa gtttttctac
     3301 actacgtttt caaattatca gttgctagaa tataagtttc ctttctatac tagcataaat
     3361 gtgtttgctc gtgcacatga attaggttta atgactgaag agcaagttaa tagagaaatg
     3421 ataagtggat acaacgcatc aaatataata agaacattaa caaatcctag atgggctggt
     3481 gacacattag aaaagtaccc ttggcttaaa gataatttaa aaactgttac aaaccgaatt
     3541 atagaaattg aatcacgtag aggtgatatg ccaacagaag tgtctcatct agctaataat
     3601 atacaatact ttgaaggtat agattatttt gtaaatattt tggtcgctct tggtaaagaa
     3661 actttccaga gaggatattc atatggtagc aattattcaa aaaaagatgt tttaagttct
     3721 ctacttaaaa attgctatcc tactaaagaa gatgattcaa aaaaattaaa aagtttactt
     3781 aaaaataccg acattactga gttaagatta atagaatctg ctatgtatgc ccctcaatgg
     3841 gtaaacataa ttgaaaaata tttaaactgg aagggtctga aaagtacaat ttggtacttc
     3901 catgcacata taaatgaagg cttctctgca caaaaagaat cagaggttgc tctattctcc
     3961 cctatttcac cacgtagttt cattgatggt gcatttgatg tagattggtt cttagattca
     4021 tataaaacta ttggggaaga aagattcaat atactttaca agtcagctaa atatataact
     4081 agtggtggta actctcatag acgtgcacaa ctctatgctg atgctacact tggtaagctt
     4141 gataaagaca tgttgatgaa agaaattgaa acaaatagaa atcaagataa attgagaagt
     4201 ttctctttac ttcctatgaa aaaaggagac ctaaaagaag caactttccg ttatgaattt
     4261 attcaaaagt ttgctaaaga aagcaagcaa tttggtgcac aacgtcgtga aagtgaatca
     4321 aaagcttgta ctatagcgct agaaaatcta gctagaagtg ctggatttgg agatgtaaat
     4381 cgtatgatgt gggctcttga gactgaaaaa ctaaatgaag ttgctcatct atttaaacca
     4441 aaagatattg atggtacttc aatttggata gatgttgatg aaagcggaaa agcatctact
     4501 aaagttcaaa aaggcaacaa gtctttaaag agtataccaa aagctattgc taaacatcca
     4561 tatgtacttg aacttaaatc tgtccaaaaa gatttaaaga gtcagtattc tcgtgcaaaa
     4621 cagagtttag aacaagctat gaaaaatgaa atagtattta cagttaaaga aattgccaat
     4681 atctataaaa atccagttat ctgccctttg attaaaaatc tagtatgggt atgtgatgat
     4741 aatattggat atgtttataa tgataagtct aagttcttta tttgcaatat agaaggtgaa
     4801 acttataagt tgacagcaaa aaataaatta aaacttgcac atccagttca tttgtttgaa
     4861 tctggtaagt ggagtgagtt ccaacatgat ttatttagta agcaacttgt tcaaccattt
     4921 aagcaagtat tccgtgagta ttatagaata aattctgatg aaatatctga aaaaactata
     4981 tctcgcagat atgctggtca ccaaattcag ccaaagaaaa ctgtagcact tcttagaaat
     5041 cagctttgga ctgtcgatta tgaagaggga ttacagaaag tattttataa agaaaatata
     5101 gtggcaaaga tgtttgcact tgcagattgg ttttcaccag cagatataga gcctcctact
     5161 attgaaacag tagaattttt tgatagaaaa acttataaaa gtttagaatt agcttctata
     5221 cctccaattc tattctcaga agttatgcgt gatatagacc ttgttgtaag tgtcgctcat
     5281 gctggtggtg ttgaccctga aactagccat tctactgtag agatgcgtat agcaattgct
     5341 aaagaacttt tattattatt aaaaattaac aatatcaatt ttgaaggctc tcatgttctt
     5401 atcacaggta atttaggtga gtattctgtg catatgggtt ctggtattgt acataaaaaa
     5461 gggatgggtg ctttgaatat acttcctgta cattctcaaa gtagaggtaa gatattcctt
     5521 ccattcgctg atgaagaccc taagactgct gagattatgt ctaaaatctt attacttgct
     5581 gaagataata agattaaaga ccctagtatc ttaagcaact tataaatata agatttatct
     5641 ctaaaacata ttgggctttc taattttcca tgaaagtcca atatgtttta tattatattt
     5701 tatacgaatt aatttataac tatagcaaat ttatatgata tcttcatcat acatattgta
     5761 tatcactgaa aatttttttc ttcattaaaa ccttttaacc ctataaaatc tgtatagaaa
     5821 atctttttct aaagatttat ttaaagtagc actttcttga aaatttataa attttatctc
     5881 taaagaagac ttatttatag gtataacctt atgtataagt cttaaattta ctatgtattg
     5941 tctatgagtc ataaagaaat taggaggtag gcgttttttt aaatcatcaa agttctcata
     6001 aaattttatc gtatcttcat ctctagtata taaattaata gctcttgaat ttttctcaaa
     6061 catgactata tcatcaaaat taatcatatg tatctgtttc cttattttat atacaaaaag
     6121 agtatcttct ataaaattat tttttgcaat tttatgagag ttaatgtggt ctattgctat
     6181 attaattgaa tcaagtattt tttctttttt aaacggctta actataaaat ctataggatg
     6241 tactttaaag ctctcaagac tatagtcagc aaaacctgtt acaaaaacta tatgtatatc
     6301 tttatctata gaaagaattt ctttggcaga atcaatccca tttttatatg gcatatcaat
     6361 gtcaataatc aataagtcta ttttctcact ttttataaat tcaattgctt ctataccatc
     6421 cttgactttc ttaacataat ctacatatcc ggactcttta actatatttg ctatatcatc
     6481 tcttacaatt ttatcatcat cacaaatcaa aacttttatc acagacttct ctcctcctaa
     6541 ctaataatat tttggtatta aaattacaaa ttcagttaaa aaatcgtcac tatttacaag
     6601 tatactataa tcatacttat ctagtatatt tttaactaga tataacccaa aacctctgtc
     6661 ttgcccttct ttagtgctaa atccatccaa aaatatactt tcgacattat gtattttagg
     6721 accattattt tttatggcta taatatactg aaattcatct tcatatgcat gtaatcttat
     6781 atacttcttt tccttttgta tagcagacaa agattctatt gcattgtcta ttatgtttaa
     6841 tagcactgaa ctcatatcat taggttcaat ctctaaatta tccataaaat cttggatata
     6901 tacatcaaaa gatatgttct tttcaagaca ttcctcatat tttaaggaag ctatagcatc
     6961 taaataagat actttcccat atcttgaatt ttttgattta aagctatttg atatattcgt
     7021 tatatattct tttgcatctt cagctaaccc agtatttaat agagaatata ctacttgtaa
     7081 atgttttaaa tagtcatgtt tttgacttct taattttacg attatattgt ccttatgatt
     7141 tattttactg tctaattttt gtagcttata ctcttgttta ttgttattgt ctagtaatct
     7201 tataaattta aatcctatat aaaagcttaa aataattaaa attaaaaaaa gtataatatt
     7261 taatactcct tcaagtaaaa aattatatcc taaacttaat tgtatatact caataccctc
     7321 catgaataaa atccatacaa aacacgatac aaaaaacgtt aatattcctc tttttaatat
     7381 gtctaattct ttataaaacc tgtatttatt cattataaga ttactaaata taaacaaagt
     7441 tagtattact ataggaaata taaaattatt ctccataatt cccctcccat ctacacttta
     7501 tatgaggatt atatcatttt agaaaaatta tgtaaaaagt caaaatttac atgtacatta
     7561 attaaacttg caaatacaca tataaatttg tacaattaag tactcaaaac ttgaagatac
     7621 ttactttgat atatatttta aataaacata taattgattt agctaagaaa ggaagtggta
     7681 aatatgaaag ataatttaaa aatagcacta agatacataa taagttataa agcaaggtct
     7741 ttagcaattg ctctaagtat aattcttgct acatcactta ttgttggtat tggtaccctc
     7801 tcaagaagtg ctcaacaagc agaggtagat aaattaaaaa gagaaactgg aagtgatcat
     7861 gtatacttta aagatataaa taaaaatcaa ttagaataca taaagtctag aaaagatatt
     7921 aaaaatttag gtataacatc tcactatggc tatacagatc ctaatgaaag attagctata
     7981 aacctagaat atgcaaataa aaattacttg actaatcaat ccaaacttat aaaaggtcac
     8041 cttccaaaag ctagtaatga ggtggtcgta gagaaatgga ttttaaatag tctgggttta
     8101 aaaccagaaa taaatcagaa cattactttt aaattatatc aaaaagaaaa accagagaca
     8161 tttaaagttg taggaatact tgaagataga cctattgaga aaaataaagg gacatgtgaa
     8221 atttttttaa atctcaatga atcaaaattg gataaatttt cttatgctta tattgaattt
     8281 aataatggca ttgatataaa tacaagcata gataatatag taaaaaatac tatgttggat
     8341 gagaatagtg ttggaaaaaa caaaatgctt atagaatcta ctagggaaaa tggtacttta
     8401 gataactcaa gtaaatacac tgcaattact atgagcctat tttctggtat tgtaatatat
     8461 agtatctatg ttatatcgat atatcaaaga atacaggaat atggtatctt aagagctatt
     8521 ggatccacta acttcaagat tttcaagttt atgttttatg aacttttcat acttgctttg
     8581 atagcaatac ctatcggtat atgcacaggt attggtggtg cacaaatatt taataggact
     8641 gcaggaaata ttcaatttga aggtaatgta acagttaccc cttttgttat tccagataaa
     8701 ataatacttt tatcaattgg aagtataata ctgacaatac taattataag tttctttaca
     8761 tatttaaaga taagaagaat ttctcctatt gactctataa gaaaaacctt tggaactgat
     8821 aaaaatataa ataaggtcaa ttctttgata tcaaaattaa ctttaaatat atcagtaaca
     8881 aaatctatat ctgctaaaaa tatctttagg aataaaaagg gcttcattat aattatatta
     8941 tccatgagtc taggaggtat aatgatcata aaggagaatt ataaatattc tttttcagat
     9001 atacaaaata aaaatggtca agaagaaaca tatatgaatg cagattttat tctaacaaat
     9061 tttttcttaa aactaaatca atctaataaa gctgattctt tcaaggatat aaaaggactt
     9121 aatgataatc agatagataa aataaaaaat ataaatggaa ttgacaaagt caaaactgcc
     9181 agcatcttag atacaagaat agaagtagat aaattaaatg gattagatta ctacaatatt
     9241 attaattcaa cgccttatta taaggatttt cctctattca taaaaaataa gaatactgga
     9301 aaatatacaa tgaaacaaaa gttaagaggt tacaatgatg agatgataaa ttcattagaa
     9361 aaatacttag tgagtgggag tataaatctg gaaagaatga aaaaagaaaa cctcgctata
     9421 ttatacgtac cacaagtttc taaaacaaat aaatacaagc taagttatac tcctggtaca
     9481 gaaacgcctg ctgtagatat aaaagtagga gatactataa aggtcaaata tccaaaaggt
     9541 gagatagatc aagatcttta tataaaacta aaagataatt atgaatatct agaatatgag
     9601 tttaaagttg gagctattgt aagttatcct tttgcagata attacctata ttctggagat
     9661 cagggtatag atgtaataac aagtaacgac tatctaaaaa aactaactgg tgtagataaa
     9721 tataatgtag tttatgtaga tattaataaa aatgcaaatg taaaaaagat aaatacactc
     9781 ttaggtgaaa taggaagtga atctcctgga actacaacag ctaatatgct aatggaaaaa
     9841 gaaaactttg ataagatgac agctagggct ctgacttatg cttatggaat agtagcagta
     9901 atgtttataa ttagtgtgtt taatataata aataacataa gttataactt aacttcaaga
     9961 actagcgaat ttggaatgtt aagagctatt ggtatctctg agagaggatt taaaaatatg
    10021 atactctatg agggaatatt atatggaata ctctcaagtg taattaccat tgtggtaggg
    10081 cttataatac agtttagaat gtattacacg tataattttg tatcatatgg attgggattt
    10141 tcaatagatt acaaaatata tatcctagtt gttttagcaa acataattgt aggtattcta
    10201 gctacatata ttccattaag gaaaataaat aaaattagta tagttgaagc tataaacatt
    10261 acagaatagg aggattaatt atgcctatat tagagattct taacttaggt aagatttatg
    10321 gaaaaaagga aacagctgta catgcactaa aggatgcaaa tttaaaaata aataagggtg
    10381 aatttgttgc gataattggc ccaagtggaa gtggaaaaag cacatttcta catttagtag
    10441 gaggtcttga gagaccaagt agtggaacta taaaagtcga tggtaaagat atatgttgcc
    10501 tttcagataa aaaattagca aaatatagaa gacaaaaagt aggctttgtt tttcaacagt
    10561 ataacctaat tccagtatta aatgtaaaag aaaatataga attaccacta aagttagaca
    10621 ataaaaaagt tgatgaagaa tacatcgaag acattataaa tttattgggt cttagtgaga
    10681 ggaaaaatca tcttccaaat caattatctg gtggtcaaca acaaagggtt gctattgcac
    10741 gcgctttatc tgcaaaacca agtataatcc tagcagatga accaacagga aatttggata
    10801 gtaaaactac ggaagaagtc atggatttac taaaaagttc tataaaaaaa tataaccaaa
    10861 cacttataat aataacacat aatgaaaata ttgctagaaa agcagataga attatatcta
    10921 taatagatgg agagcttaaa ttaacactct aaaactattt atagaaaaga ggatttaaat
    10981 gttattgatt ttagataatt taaatttaaa cttagatttt gactacagaa taataagaga
    11041 agaaaatgat gatgtagata tatttataga tattaattat cgtagcttag atatagatac
    11101 tgatggttct aacttattta attctagaat acaatttcca tttgtaagag cattgattct
    11161 aaggataaat aagaacaatc aatgtatgac aattcatttg ttaagagata tagatttatt
    11221 ctctgctttt gccaactttg aagtggatta tactgactct ataataaata taaaaaatca
    11281 aaatgagaaa gtaattttaa ataaatctat caaaaaatag taagggggcc ttaataggcc
    11341 ccaatttttt tctt
//
LOCUS       DAELXX010000116        11329 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.117, whole genome shotgun sequence.
ACCESSION   DAELXX010000116 DAELXX010000000
VERSION     DAELXX010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11329
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.117"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            465..1076
                     /locus_tag="KPH29_003054"
     CDS             465..1076
                     /locus_tag="KPH29_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468629.1"
                     /translation="MNIKRINLLLSTVMAAVIISLPLAVTANAMEKTESDNNVKIELS
                     DKEVEELHNYWPEDKIFSDEKIDEILIKTGQFTQKELDKINQEVDDSAKKVNQNSRVK
                     YINGYNKYRTFTKNGKNYLYIYVSGKTLKKIKAGTDLASTFGGFLPKIWVELDVVAIG
                     KIITINMDGINTNYGIVLSYIEDKWNFQGATYTYRYDHWFYQT"
     gene            1104..1286
                     /locus_tag="KPH29_003055"
     CDS             1104..1286
                     /locus_tag="KPH29_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468630.1"
                     /translation="MKKRLYKVFLKTIPNLIIVILSLALSFYTNTFDIDLKILLLIPL
                     AYVLLFIYYYIKTKKD"
     gene            1526..2068
                     /locus_tag="KPH29_003056"
     CDS             1526..2068
                     /locus_tag="KPH29_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG1468631.1"
                     /translation="MLIYLAILELEEDKIKFEKVYEKYRQIMFYVANKILKDDHLSED
                     AVHNAFLRIIKNIDKIDEVDSPRTKAFIVIIVERIAIDFYRKRKREKVSDIEEEYKNR
                     EINFSIEDKVCESNLAIALAKLNESYFQVLSLKFQYGFSNKEIANALNLGEENVYKRI
                     QRARKKLKEILEEMEVGNIE"
     gene            2100..2762
                     /locus_tag="KPH29_003057"
     CDS             2100..2762
                     /locus_tag="KPH29_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4367 domain-containing protein"
                     /protein_id="HBG1468632.1"
                     /translation="MLRESIPIATKIIIDDLLSDDELNHVFSKGFERKMKKLIKQQKR
                     SSLTNKIIFYSKQTAIVFLIVLASLLTITMSSEALRSRFFRLVIRVYEDLTSFVFSTN
                     GEDNLSLKNKEPEYIPKGFKEVDRVDDFVITYRNDENIEIQYVTDKISSNSIILDTEN
                     AKVKDIFVNGHKAKYIVKGTFLQLFWNDNNFIYLLNVDYQNSGESEFYEDILIKIAKS
                     IK"
     gene            2952..3269
                     /locus_tag="KPH29_003058"
     CDS             2952..3269
                     /locus_tag="KPH29_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468633.1"
                     /translation="MKSDLYLCKSNKLYRDNEVLEKFMEDLSFLIDDYKFSLETISIL
                     CKINIEKLTNFYAGKGYILYEEMSLIESVLAPFLSAIDIAKHNYDVVIKSRESDKPLI
                     FEN"
     gene            3291..3734
                     /locus_tag="KPH29_003059"
     CDS             3291..3734
                     /locus_tag="KPH29_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468634.1"
                     /translation="MKRLFALLLTLSLVILPSTLVFANEKAENDSIYAEAPLVFQSNE
                     KAYTHGSGGTAYIGYMSSKHLNWRITSSKGIVILFSGTVEIRNSKNMLVASYPISGGG
                     SSTISGQIDVKYLKKGDYKAILKGTSTTGGGVLVVKSGLVQMFTI"
     gene            3952..4395
                     /locus_tag="KPH29_003060"
     CDS             3952..4395
                     /locus_tag="KPH29_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468635.1"
                     /translation="MNLRKTVISGMCALVCSSAVAIPAFADEYQDKNIEQYSAVNYID
                     DTMVKMSYIKSGTCNLSISGSTAKVTCFVRGASNVTSTSIIARLQKSNDNGKTWSTIQ
                     TWNASGNISCSLSKSKTIGKGYYRVHSTIKANSESKNIISSTKKY"
     gene            complement(4526..4946)
                     /locus_tag="KPH29_003061"
                     /pseudo
     CDS             complement(4526..4946)
                     /locus_tag="KPH29_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894307.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            complement(4924..6201)
                     /locus_tag="KPH29_003062"
                     /pseudo
     CDS             complement(4924..6201)
                     /locus_tag="KPH29_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899679.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(6224..6766)
                     /locus_tag="KPH29_003063"
     CDS             complement(6224..6766)
                     /locus_tag="KPH29_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468636.1"
                     /translation="MLLCDPASAGGSKHDYSAFLVGSESENGLLCGRLAELAKINART
                     DFDKYILHMIYLLKVYPDITHVYIEKNTFNGTAANQLELKIKNDDVLYYRDIEIINEH
                     QKKNKDDKISTLIPVLNKGQMIFAEEDKAFIQQILNFTGQKYSLHDDAPDISAEFINR
                     IFNIKVNESITLLDRRNLGL"
     gene            complement(6806..7024)
                     /locus_tag="KPH29_003064"
     CDS             complement(6806..7024)
                     /locus_tag="KPH29_003064"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468637.1"
                     /translation="MLFDFKNTNHLEDAKEFYWENEKEIKFPILWPSFWNCLDMALSY
                     YENPTSFKQEVQNDVNSIGEKWFKTIRT"
     gene            complement(7220..7963)
                     /locus_tag="KPH29_003065"
     CDS             complement(7220..7963)
                     /locus_tag="KPH29_003065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468638.1"
                     /translation="MDNLERNIQLIFSYMAKTFIRNGATIREAEKEANEIIKTNSSNL
                     WGVNGLAYQLGKINLEFFCMYFIQDTYLPKEDNVAAPIAKVHHELWEDIQESIIGDGS
                     QQLGRIYPRGTGKSAFGDLATTVWSHCYKHKTYTLICSDIGSTAEKFVKDIKNALLEN
                     EYIKKAFGVLLNDNDRKYICNSTQLELTNKTFIEAISSSSPMRGKKYNNNRPDLIILD
                     DYQSEENVRTEDAREKKFKKFSDDVKYAA"
     gene            complement(8263..8820)
                     /locus_tag="KPH29_003066"
     CDS             complement(8263..8820)
                     /locus_tag="KPH29_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG1468639.1"
                     /translation="MVVGYMRVSTAEQNEDRQLVTMKKHNVEKMYQEKISAKDTKRSK
                     LNAMLDFVREGDTIIVHDFSRLARSTKDLLEIVELLEKKHVKLISDKENLDTSTPTGK
                     LMLTMLGAIYEFERTNMLERQREGISIAKEQGKYKGRKEVKIDNFGSYYDRYKTRELN
                     KTQLAKELGVSRPTLDRLIKSHESN"
     gene            complement(9015..9203)
                     /locus_tag="KPH29_003067"
     CDS             complement(9015..9203)
                     /locus_tag="KPH29_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468640.1"
                     /translation="MNNKKKSFLISGTINVILLIIVFALFSYNIIENSTRNMIITVIL
                     IFIFEIIKIKILEKYYNL"
     gene            complement(9214..9789)
                     /locus_tag="KPH29_003068"
     CDS             complement(9214..9789)
                     /locus_tag="KPH29_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468641.1"
                     /translation="MLKKKIISFLMAGIFVLSSGYNLVYASTFEENEYKQELVNENEF
                     TIINSNENEMIVDKKGNNTYVSTKVTPGSWKNVKYLGQKSRKLSSPYTRERIIQTIQW
                     GLGYLSGGSSFLLGLAESIYREKVYDITKYGGVYTKTYYRRQITDIRNSHANVPVPYR
                     YEYVTYFYSSSNFKKVVYTERVGDGIKLANN"
     gene            complement(10122..11261)
                     /locus_tag="KPH29_003069"
     CDS             complement(10122..11261)
                     /locus_tag="KPH29_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG1468642.1"
                     /translation="MVIKLTIEEYKNKYSTGDKIILSLKSFDNKELYYHETDNILNSK
                     DEIDRLTSSKIFKIIEDKNDLALESYYSSVIEGAFSTRKIAKSIIRGKMKPSNKSEYM
                     IYNNHRALEYGLDNLDKLYSHKFIYNLHHILGENCLDSEEYEYRTEKVYVCDSKGEII
                     HTGLDPLKIYNFMDELIIFMENSKVNDLVKSTIIHFYFVYVHPFSDGNGRTSRALSYL
                     YLINKGYDTFKEFSISYIISKNRTKYYKAILDVENKGYNLTVFIEFMLKSIIQSINEM
                     RDMHDRKSLESILKEELFENDITLSATEEHILKYICSKDNYSMTLENYIKKNKGRYLK
                     AGIKEIELVDQLMEVFNNLEEIEILSREKDVYKVNEKYLNMLEIS"
BASE COUNT         4014 a         1538 c         1374 g         4403 t
ORIGIN      
        1 tcaaaaaaat taagaaattt tcttaaaggg ataataattt ttacaaaaat ggaaaaaata
       61 tcaaaataag gctatgattt ttaaagccat agccttacca ataaatgttt aattatcact
      121 aaaaaaataa atttttattc aaagaataca cataaatatt tccagactca cttattgtat
      181 aagttatatt ctattttgta tgaagtcaat taaaaactga actatattaa aattaatttt
      241 ataaataaca aatataaata attacaaaac cttaactgcg ttaagtttga aatataataa
      301 tattaaaaac acttctctca aatcaagtat cttttgttat attttcataa gttttaaaaa
      361 tataaatttt ttaaaacttg tgtccaaaaa acaaaattaa atatgttata tatgtaaagg
      421 gttaatttta aaaccttaaa tttattatag ttagggggat ttaaatgaat atcaaaagaa
      481 ttaatctcct tttatcaaca gtgatggcag ctgtgataat atctttacca cttgctgtta
      541 cagcaaatgc aatggaaaaa acagaatcag ataataatgt gaaaatagag ttgtctgaca
      601 aagaagtaga ggaattacat aattactggc ctgaggataa aattttttca gatgaaaaga
      661 ttgatgagat cttaattaaa acaggtcaat ttactcaaaa ggaattggat aaaatcaatc
      721 aagaagtgga cgattctgct aagaaagtaa atcaaaattc tcgtgtgaaa tatataaatg
      781 ggtataacaa atacagaact tttactaaaa atggtaaaaa ctatttatat atttatgtaa
      841 gtggaaagac attaaagaaa ataaaagctg gaacagatct tgcatctact tttggaggtt
      901 ttttaccaaa aatttgggta gagcttgatg tagtggcaat tggaaagata ataacaatta
      961 acatggatgg tataaatact aattatggga ttgttttatc atatatagag gataagtgga
     1021 attttcaggg tgcaacatat acttatagat atgatcattg gttctatcaa acttaaaaga
     1081 catgcttttt taggaggaat aatatgaaaa aaaggcttta taaggttttt ttaaaaacaa
     1141 ttccaaacct gataattgtg atattatcac ttgcattatc attttatact aatacatttg
     1201 atattgattt aaaaatattg cttttaatac ctttagcata tgtacttttg tttatatact
     1261 attacatcaa aacaaaaaaa gattaatttt tatggaatta gatatatacg aaaataatta
     1321 atttaaaaat ttaactttga gaatcatttg tagagttaag gcccttataa tcccgaaaat
     1381 ggatttgaag ggccttttta ctagatatct ttaaaaagct tgttaagcat agttatattc
     1441 tatttaagta gagttaatta tatatttatg ttataattga actttaaaat attaattcag
     1501 aatttttata ttggaggaat tctaaatgtt aatttatcta gctatacttg aactcgaaga
     1561 agataaaata aagtttgaaa aggtatatga aaagtataga caaataatgt tttatgtagc
     1621 caataaaata ttaaaggatg atcatttatc agaagatgca gtacataatg cttttttaag
     1681 aataattaaa aatatagata aaattgatga ggtagacagt ccaagaacga aggcttttat
     1741 cgttataata gtagaaagga ttgctataga cttttacaga aagagaaaaa gagaaaaggt
     1801 atctgatatt gaagaagagt ataaaaacag ggagataaat ttttctatag aagataaagt
     1861 atgtgaaagt aatttagcta tagcacttgc aaaacttaat gaaagttact ttcaagtgct
     1921 ttccttaaag ttccagtatg gtttttcaaa taaagaaata gcaaatgcat taaatttagg
     1981 ggaggaaaat gtatataaga gaattcagcg tgctagaaaa aaattaaaag aaatattaga
     2041 agaaatggag gttggaaata ttgagtaata aaaaagatga ttttgatatt acggatgata
     2101 tgcttagaga aagtatccca atagcgacaa aaattattat tgatgacctt ctttcagatg
     2161 atgaattaaa tcatgttttt tcaaaaggtt ttgagcgaaa aatgaaaaaa ctcattaagc
     2221 aacaaaaaag aagtagtctt acaaataaaa tcatttttta ttcaaaacaa acagctattg
     2281 tatttttaat agttttagct agtttactta ctattactat gagtagtgag gcattgcgtt
     2341 cacgtttctt tagattagta ataagagtat atgaagactt aacttcattt gttttttcaa
     2401 ctaatggaga agataatctt agtttaaaaa ataaagaacc tgagtatata ccaaaaggat
     2461 ttaaagaagt tgatagagtt gatgattttg ttattacata tagaaatgat gaaaatattg
     2521 agatacaata tgtaacagat aaaattagtt caaattctat tatattagat acagaaaatg
     2581 caaaagttaa agatatattt gtaaatgggc ataaagcaaa atatatagta aaaggaactt
     2641 tcttacagtt attttggaat gacaacaact ttatatattt attaaatgta gattatcaaa
     2701 atagtggaga atctgaattt tatgaagata ttttgataaa aatagcgaaa agtataaaat
     2761 aattttttta agtgtccaag ggtagcctct tcattcgtta tacaagtata tatgaaaatg
     2821 tgaggtgatt tttatggttt taaaatctac atttacctga gcgttaaaaa tattgttata
     2881 ggaaaaatta atacattata gttataacat gcttgcaata ggaataaaac tttaaaattg
     2941 aggtgtaaaa aatgaaatca gatttatatt tatgtaaaag taataaattg tatagagaca
     3001 acgaagttct agaaaaattt atggaagatt taagttttct tatagacgat tataaattct
     3061 cgttagaaac aatatctatt ctatgcaaaa taaatattga gaaattaact aatttctatg
     3121 caggaaaagg atatatactg tatgaagaaa tgtctcttat tgaaagtgta cttgcacctt
     3181 ttttaagtgc aattgatatt gctaaacata attatgatgt tgtcattaaa tcgagggaaa
     3241 gcgataaacc tttgatattc gaaaattaat ttaaaactgg gggaatacac atgaaaagat
     3301 tatttgcgtt attacttact ttaagtttag ttatattacc atcaacttta gtatttgcta
     3361 atgaaaaagc tgaaaatgat tcaatttatg ctgaggcgcc attagtgttt caaagtaatg
     3421 aaaaagcata tacacatggt agtggaggaa ctgcatatat aggttatatg tcaagtaaac
     3481 atcttaattg gaggataaca tcttcaaaag gaatagttat actattttca ggaactgttg
     3541 agattagaaa ttcaaaaaat atgcttgtag catcatatcc aataagtggt ggaggatctt
     3601 caactatatc tggacaaata gatgttaaat atcttaaaaa aggggattat aaagctatat
     3661 tgaaaggtac aagtacaaca ggaggcggag tcttagtagt aaaatctggt ctagttcaga
     3721 tgtttacaat ttaatattat aagtaaaata aaaatcatga atttattggg ggatagtcac
     3781 aatacatatt atttacagat attcataact attatagtta aagacattct attataaaga
     3841 aaatgtataa tttaaaatta taataaaaaa aattatcatt agtgtccaaa aatcacttct
     3901 tcattcgtta tataagttat ataaatagaa aacaaatgaa gaggtgattt tatgaatctt
     3961 agaaaaacag ttataagtgg tatgtgtgct ttagtatgtt catcagcagt tgcaatacct
     4021 gcatttgctg atgaatatca agataagaat attgaacaat attcagcagt aaactatata
     4081 gatgatacta tggtgaaaat gtcttatatt aagtctggta cttgcaattt aagtatttca
     4141 ggaagtacag ctaaagtaac atgctttgtt agaggagcat caaatgttac aagcacatca
     4201 attattgcta gattacagaa aagcaatgat aatgggaaaa catggtcaac aatccaaaca
     4261 tggaatgctt caggaaatat ttcatgttct ctttctaagt caaaaactat aggaaaagga
     4321 tattacagag ttcattccac aataaaagct aactctgaaa gtaaaaatat aataagtagt
     4381 acaaagaaat attaaaaata gatattgtat ttatgagttg tagtaaacaa gaataatagt
     4441 tttttaaaag caaatataat taattttaaa tagagtgcca atataaaata ggtgtataat
     4501 atttatactt ttttatattg gcactctatt ttataaaata agttattata gaaaatgaaa
     4561 cataatttat atataaattt tctgtttgac ctggtatata atcttttata tcacatatca
     4621 gtattttccc atcattataa ccccaaccaa tcacagttgc aggtatcata tcagcttctc
     4681 catcataaat gattgtatgt ttatacatga tttttccctc gctattatct atagttttat
     4741 tcaaaatacc ttctgctatt aatttagcaa ctatgtcttt aatagtctgt atctgcttta
     4801 ctgtctacaa aacatacttc aattaatatc gctggagctt ttgtatgact aagccaatac
     4861 aattccctta cgtccgattt tgcacctcta ttttcagcag atttatatat atcttcagtc
     4921 aatttagtta taatcttttg attcatctaa taaaatctct ccctctgaaa ctatttcctg
     4981 ttctttttta gccttttcca tttctgcatg aggattatca ataaaacttg gttgagataa
     5041 tccggtttca gtagataatt tatctccaag ttgacttaat atatttgcta caactaaatc
     5101 attatgtggt atcttagggg taaatttagc cttaatatct ttataatcat agtcaaaacc
     5161 atatttttta ttaagccaaa tgcacaataa tttgtaccta ttaaataaag tatctgcaat
     5221 actatcatta ttctggctac aaatccattc tgtactgatt aatcttgcta caactgctaa
     5281 acttgatgta ttactttgca ttttttcatt gtgatttata tgagatgtaa ttttatatat
     5341 gttttctttt attgaagata gtgtattttg aacaaatgta tcattcatgt ctttaataag
     5401 ccattctatt tttccatctc cattaacttg aagtattcca agttctttca tccttggcaa
     5461 atcatcttct ttaatattac attcagagaa ggttaaataa gcatttctaa aatcactaat
     5521 ttcattactt atatcactta aattagtttc ataagcatct tgtaatcctt ttatatcatt
     5581 gaatattgta ttttttccta gttcttcact tcttttacaa attgatattg gaacattacc
     5641 attaaatata ttatttactg taggtttttc tatctcttta aaatttgaat caaaatgata
     5701 tatgtattct ttatcatata catctatata aatatcatct ttaaaatctt ttttaaattc
     5761 tctaaaaaaa tagatggtct ctccatcttc attttcataa tggcagccat ctgttggttt
     5821 tattattctt actttcattt cattgttttt tacatagtaa agttcatagc aaaatccaaa
     5881 taatagtgta tatctcaata aatctgaatc gtgcttttta ctccaatggc aagtatagta
     5941 ttctaaatca tttataatct tctcatcacc taatctactt gtataggtga ttttatttgc
     6001 aagagaataa gcgacctctt cattaataaa cttttttaag aaattagtgt ttatcttatt
     6061 attagacctc ttggtaacca ttttatagtt actaatagca tctgtttcac ctttataata
     6121 cttatacata gtgttgtatt cttgcttttt taaactctag tcttcatata tttctcttaa
     6181 taaacttaaa tctatttcca tttaatgcat cacctccata caatcaaaga cctaaatttc
     6241 ttctatctaa tagtgtaata ctttcattta ctttaatatt aaaaatccta tttataaatt
     6301 ctgctgatat atctggtgca tcatcatgta gagagtactt ttgtcctgta aaattaagga
     6361 tttgctgtat aaatgcctta tcctcttctg caaaaatcat ttgaccttta tttaaaacag
     6421 gtattaaagt agagatttta tcatctttat ttttcttttg gtgctcatta atgatttcta
     6481 tatctctgta gtaaagaaca tcatcatttt ttattttaag ttctaattga ttagcagctg
     6541 taccattaaa tgtatttttt tctatataaa catgtgttat atctggatac acttttaata
     6601 aataaatcat atgcaatata tatttatcaa aatcagttct agcatttatt ttggctagtt
     6661 ctgccaatct gccacatagt aaaccatttt ctgattcact tccaacaaga aaagcactat
     6721 agtcatgttt agaaccacct gcagatgctg ggtcacataa taacattgtc tttttgaatg
     6781 tatgagtttc tatacattct ctagattaag ttctgattgt tttaaaccat ttctcaccta
     6841 ttgaatttac atcattttgt acctcttgtt taaaagatgt tgggttttcg taataactta
     6901 aagccatgtc taaacaattc caaaaacttg gccataatat aggaaattta atttcttttt
     6961 cattttccca gtaaaattct ttagcatctt caagatgatt tgtgtttttg aaatcaaata
     7021 acaactcttt gaaattaagc cataaaccag aattaaataa ttcatctaca ttatcaacta
     7081 aaactccttt ttcacattta aatttccaag ttggtaaatt ctttagccta ctatagaaac
     7141 attctttatg ttgtaaggtt cctaatgcta taaaagttgt tcctcttttt ataatcttac
     7201 catttcttat tactggtttt taagcagcat attttacatc atcagaaaat tttttaaatt
     7261 ttttctctct agcatcttct gttctaacat tttcttctga ctgataatca tcaagtatga
     7321 taagatctgg acgattatta ttgtattttt ttcctctcat tggtgatgaa gatgatattg
     7381 cttcaataaa ggttttatta gttaattcta attgagtact attgcaaata tattttctat
     7441 cattatcatt taagagaact ccaaatgctt ttttaatata ctcattttca agtaatgcat
     7501 tttttatatc ttttacgaat ttttctgctg tagagcctat atcagagcaa attaaagtat
     7561 atgtcttatg tttataacaa tgtgaccata cagttgttgc taaatctcca aatgcacttt
     7621 tacctgttcc tcttggataa attcttccta attgctgtga accatctcca attatagact
     7681 cttgtatatc ttcccataac tcatgatgaa ctttagctat aggagcagca acattatctt
     7741 cttttggtaa atatgtgtct tgtataaaat acatacagaa aaattctaaa tttatttttc
     7801 caagctgata agctaggcca ttaactcccc aaagattgct tgaattagtt tttataatct
     7861 catttgcttc tttttcagct tctcttatag ttgctccatt tcttataaag gtctttgcca
     7921 tataactaaa tattaattgt atatttcttt ctaaattatc cactcgtatt tacttcctct
     7981 taattcttct atttttttag gttcagtgag taatacaatt aataaaataa acaatgcaaa
     8041 tttttttcta ataaaatcac cctctttgaa atatgtctct ggtttttaat ttttgctaga
     8101 aaatttctag aactcatttt ctataaataa tacttttttg aaatagaagg atacccccta
     8161 tactgaaaaa cagttattta taattatttt aaacataatt aaagggcata attctatgaa
     8221 tttgccccac taaaattaac ttaagcatat atattgctct tcttagttac tctcatggct
     8281 ctttattaat ctatctaatg ttggtctact tactcccaat tctttagcca attgagtctt
     8341 attaagttct ctagttttat atctatcata ataactacca aagttgtcta tcttaacttc
     8401 tttacgtcct ttatatttac cctgttcttt agctatagag ataccttctc gttgtctttc
     8461 taacatgttt gttctctcaa attcatatat tgcgccaagc attgtaagca taagttttcc
     8521 tgtaggtgta cttgtatcta aattttcttt atcactaatt aactttacat gtttcttttc
     8581 taagagctca actatttcta ataagtcttt agtacttcta gcaagtctac taaagtcatg
     8641 tacaattatt gtatctcctt ctcttacaaa atctaacatt gcgtttagtt ttgatctttt
     8701 agtatctttg gcacttattt tctcctgata cattttttct acattatgct ttttcattgt
     8761 aactaattgt ctatcttcat tttgttctgc tgttgaaact ctcatataac ctactaccat
     8821 ctttgatacc tccacctatg tttactctat aaataatatt atagcataaa tgtaaaacta
     8881 gagttaaaga tttgccatgc tttttgtaaa ttaattggag ttttgatttt atttttacat
     8941 cagaatagat agtttgtaat gtaaatttgt accagcatac tttacaatat gctcttagga
     9001 aagatatttc ttgtttatag attataatat ttctctagta tctttatttt tataatttca
     9061 aaaataaaaa ttaaaattac agttattatc atatttcgtg tactattttc tataatatta
     9121 taactaaaca atgcaaaaac aatgattaat aaaattacgt ttatagtgcc agaaatcaag
     9181 aatgattttt tcttattatt catatatttc ctcctagtta tttgctagtt ttataccatc
     9241 tcctactctt tcagtatata caactttttt aaagttagat gagctataaa aatatgtaac
     9301 atattcatat ctgtatggaa caggaacatt agcatgactg tttctaatat cagttatttg
     9361 tctcctatag tatgtttttg tatatacgcc tccatattta gtaatgtcat atactttttc
     9421 tctataaata ctctcagcta aacctaataa aaaagatgac cctccagaaa gataacctaa
     9481 tccccattga atagtttgta taattctttc tcttgtataa ggagaactta atttgcgtga
     9541 tttttgacct agatatttaa catttttcca actaccaggt gtaacttttg ttgaaacata
     9601 agtgttattg ccttttttat ccacaatcat ttcattttca ttgctattaa taatagtaaa
     9661 ttcgttttca ttaacaagtt cttgtttata ttcattttct tcaaaagtag atgcataaac
     9721 caaattatat ccactagata aaacaaatat tcctgccatt aaaaaactta ttattttttt
     9781 ctttaacata atattttact acctttactt aaatatttat tctataaaca ctgtcatata
     9841 acctgttagt tatattaaaa ataatctttt tatataactt cacaatcaaa ccttgtctta
     9901 tttcataata gttttattta atatcatata atttagacct ccttataaga ttatttatat
     9961 atataacgaa taataaaagt aattttggac aaaataaaaa tctttttatt ttaagaatga
    10021 aaaatttaac cctctaagaa aactcaatta agagcgattc atagagggct tattattata
    10081 ttatataact ttatatactt tttattttta tatgcttcaa tttaacttat ttcaagcata
    10141 ttcaagtact tctcattaac tttatataca tccttttctc tagataaaat ctctatttct
    10201 tcaagattat taaacacttc catcaactgg tcaactaatt cgatttcttt tattccagcc
    10261 tttaagtatc tacctttatt ctttttgata tagttctcta atgtcataga ataattatct
    10321 ttactacaga tatattttaa tatatgttct tctgttgctg atagagttat atcattttca
    10381 aataattcct cttttaatat actctctaaa gacttcctat catgcatatc acgcatttca
    10441 ttgatagatt gtattataga ctttagcatg aactctataa atacagttaa attatatcct
    10501 ttattctcta catctagtat agccttataa tacttagttc tgtttttaga tattatatat
    10561 gatattgaaa actctttaaa agtatcatat cctttattaa ttaaatatag atatgataag
    10621 gctcttgatg ttcttccatt accatcacta aaaggatgaa catatacaaa gtagaaatga
    10681 attattgtac tctttactaa atcatttact ttactatttt ccataaaaat tattaattca
    10741 tccataaaat tatatatttt caaaggatca agtcctgtat gtataatctc accttttgaa
    10801 tcacatacat atactttttc agttctatat tcatattctt ctgaatctaa acagttttca
    10861 cccaatatat gatgtaaatt atatataaat ttgtgactat ataatttatc taaattatct
    10921 aatccatatt ctaaggctct atgattattg taaatcatat actcactttt attacttggt
    10981 ttcatctttc ctctaattat agattttgca atctttctag ttgaaaaggc accttctatt
    11041 acactagaat aataagactc taaggctaaa tcatttttat cttctattat tttaaatatc
    11101 ttgcttgatg ttaatctatc tatttcatct tttgaattta atatgttatc agtttcatga
    11161 tagtatagtt ctttattatc aaaactcttt aaggaaagta taattttatc tccagtagag
    11221 tatttatttt tgtattcttc tattgtcagt ttaatcacca ccttagttta tctttatcta
    11281 ttaatattaa caaaaagtat tagtttttac actatttttg attgtttat
//
LOCUS       DAELXX010000117        11175 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.118, whole genome shotgun sequence.
ACCESSION   DAELXX010000117 DAELXX010000000
VERSION     DAELXX010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11175
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.118"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            121..771
                     /locus_tag="KPH29_003070"
     CDS             121..771
                     /locus_tag="KPH29_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HBG1468643.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKEIVKKMGNTYEILNYDELHKLAFEVKEFLEDE"
     gene            complement(911..2287)
                     /locus_tag="KPH29_003071"
     CDS             complement(911..2287)
                     /locus_tag="KPH29_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG1468644.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(2303..4216)
                     /locus_tag="KPH29_003072"
     CDS             complement(2303..4216)
                     /locus_tag="KPH29_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG1468645.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            4352..4984
                     /locus_tag="KPH29_003073"
     CDS             4352..4984
                     /locus_tag="KPH29_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBG1468646.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            5123..5662
                     /locus_tag="KPH29_003074"
     CDS             5123..5662
                     /locus_tag="KPH29_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBG1468647.1"
                     /translation="MVFVEELWKKAIEFHGHECPGLAIGFKAALAARKYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FQMDREGYKKYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFNKYERFYEEY"
     gene            complement(5838..6008)
                     /locus_tag="KPH29_003075"
     CDS             complement(5838..6008)
                     /locus_tag="KPH29_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG1468648.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
     gene            6293..7360
                     /locus_tag="KPH29_003076"
     CDS             6293..7360
                     /locus_tag="KPH29_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HBG1468649.1"
                     /translation="MKNKLVYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     KREPVKKDITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSIEAVSIANNHSEDYFEEGMKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSSSRNNYQPKVL
                     DGSEKDRVLNKLEKISKDLNK"
     gene            7530..9431
                     /gene="cooS"
                     /locus_tag="KPH29_003077"
     CDS             7530..9431
                     /gene="cooS"
                     /locus_tag="KPH29_003077"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBG1468650.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAEYIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            9424..9897
                     /locus_tag="KPH29_003078"
     CDS             9424..9897
                     /locus_tag="KPH29_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG1468651.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSKHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            9872..11098
                     /locus_tag="KPH29_003079"
     CDS             9872..11098
                     /locus_tag="KPH29_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG1468652.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRTLKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDVCY
                     ADFFNIKENGEFCYSV"
BASE COUNT         4278 a         1372 c         1840 g         3685 t
ORIGIN      
        1 gagtagaatg aatttgtcaa gtttaattta gttgtaattg tatcttttat taaaaataat
       61 ataaattaaa acatataaat aaaatatttt atacaatata ttgtaaagga ggtaactttg
      121 atgaaagcag aaaaaacaag tatatatcca attattaaaa ataatttaaa caaaatatca
      181 attgttgaat ataaaaaggg tcaaagattt gtagcatcag ataaagactt acaagaagtg
      241 ttctttataa tagagggagt tgcattagtg gagtgtacta ctagaggagg aaataaattt
      301 cttgttgaca ttgtacctga aaatgaattt ataggcaaaa tcagttacat atatgaacat
      361 aatttgaaat gtgatatatt tgcaaagacc aatataaaat tatttcgatt tgaaaaaaat
      421 gtatttgaag agttttatct caaaccagat tttattgcac tatttcacag aaaatgtaca
      481 agaagaatat atgaattata taaaaccaga atggtaagag aactattttc ttgcacagaa
      541 gttattgcct attgtatatc aaataaacaa gaaaataatg tctgcaatat aaaagctatt
      601 cgtaatttca gtgaaatcta ttcaattagt agaaaaagta gataccattc tctgctaaag
      661 ttaactgaaa aggagatagt aaaaaaaatg ggtaatacat atgaaatttt aaactatgat
      721 gaattacata aattggcatt tgaagtaaaa gagtttttgg aagatgagta aaaattgtta
      781 cttaatcaaa ataaagtata ataatttctg taaaaaatag aaatagtctc actgaaaatc
      841 aaaaatactt tagttatttt caatgagact gtttagaaat attgtatact tataatgtat
      901 agtataatta ttaatcaatt tcctttacta tattaatagc ccatttttta gataatgctc
      961 taggaacacc aaatataaac ttaaccactt cttcactata agtgagtaaa acgagtacaa
     1021 atataggtgc acctttaaat gcagctataa aagttagagg aatagaagct aaccacatac
     1081 atcctaattc tagaaataga gcatatttag tatctcctcc acttcttaaa ataccaatta
     1141 taaccaatgt attaaaagat ttaagagcca ttagtattcc catgataaag aatatcttag
     1201 ttatatcaga agctaagcta tcagaaacac taaacatttt taataataat ggtatattta
     1261 aaattaaaat aattccaagt attaaaccag ctgaaaatac aagtatagaa aactttttag
     1321 catattcaat ggctctttta atatggtcag ctccaagttc atttccaagc ataatagatg
     1381 caccagatgc aatacataca gctgtcatta tgaagaaatt acctgtactg gttgctatct
     1441 ggctagctgc tatagcagaa gtaccagctc ttgcatatgc aacagaatac aggacagttc
     1501 caattgccca tacagagtca tttacaaata taggaaagct ttttgatgaa aaagatttta
     1561 taaaaatttt atcaatagct tttagatttt ttagaccaaa ctttaatata tagtcttttt
     1621 tataaaggta aacataagta atcattaaaa ttaattcaca tattctagca ataacagtag
     1681 ctagagcagc accttttaca cccaatgcgg gaagaccgaa gttaccaaat ataaatccat
     1741 aatttaaaat aacatttgtt acaagtgcaa atgcactaca aatcatacct aacttaggat
     1801 ttctaacacc tcttgaacca gtactaaata cagtacttac agcaattaat ggataactaa
     1861 atactgcaat actaaagtaa tctatacaaa gttttaccac ctcagaatca tacgaaaata
     1921 tgtgtataat tggctttggt gttagtaaag caggaataac aaatacaaaa cttaaaacaa
     1981 ttgccaatac acaagtaaga cctgtgactt ttcttatatt actcacatcc ttctttccat
     2041 aaaactgtga aataaaaact ccagcacctc ctgtgatacc agataaggac atattaaaaa
     2101 gaaagaaaaa ttggtttgca actccaactg aagctacaga tgtctccccc aaactactta
     2161 tcataattgt atctattaca tttacagaag tagaaattaa gttctgtatg ataataggta
     2221 tacataaaga tagaagtatt ttataaaatc ttttattttc gtcttttaaa gaaaatagtt
     2281 cttgcaaata atcctctcct ttttacataa attcttccca ctcatcatat agagaggcaa
     2341 ggttattttc taaattaatt ttttcttggc taacttcttt agctttatct ggattagagt
     2401 atacttcttc ttgacataaa agtatatcta atttttcaat ttcaatctct aacttttcaa
     2461 tttctttttc tatattttgt ctttttattc tatttttctt ttctatttct ctttgttctc
     2521 tctcttttct tttttcatct tttatttgag ttctagtttt ttcttctttt tcttctataa
     2581 tactcatctc ttggatttgc ctttttttat ctatataata atcataattt cccagatatt
     2641 cagtaattcc attttcatct aaaactaaaa ttttatcaac aacagtattt agaaagtatc
     2701 tatcatgtga tattgtaaat atagtcccat cataattagt taatgcctct tcaaggactt
     2761 ccttagaatc aatatctagg tgattagtag gctcatctaa taacaataag tttgcatttg
     2821 atagtataag tttaagtatg gcaactctag ctctttctcc accactcaat gtagaaatct
     2881 tcttaaaaac ttcttcatct acaaataaaa atgcccctaa catatttctc aaagtagttt
     2941 gagtaagatg agtattattt tcccaaatct catcaattat tgtgttatct aaattaagag
     3001 ttttttgttc ctgatggaag taggaaacat gtacattagt tccaaattta atattgccac
     3061 taagaggttg tagttcatta gttattattt taaataatgt agatttgcca acaccatttg
     3121 cacctataag tgcaactttt tcccctctat atatatctaa atccaagtct ttaaaaagta
     3181 ttctttctcc ataacccata gatacatctc taatttgaag aacatcatta ccactctgga
     3241 cagaaggcgt aaattgtata cgagcttttt tcctataagc ttctggttta tctaaaacgt
     3301 ctaccttgtc taatgctttt tctttacttc ttgcacgctt taggtgtttt tctctaccat
     3361 aagcttttag tctttcaatt gattcttctt gcttttttat ttctttttgt tggtcttcaa
     3421 atttcttaag ctctagttct ttttctattt tagataactc aacaaattta gagtagttac
     3481 cattatatac tttaagtttt ttgttgtgaa cttcaaaaat tctatttacg gattggtcta
     3541 agaaatatct atcatgagat attaacatga cagttccttt atattgtttt aaaaatactt
     3601 ctaaccattc tattgcttca gagtctaagt ggttagtagg ttcatcaagt aacaatagag
     3661 ttggtttctt aagaagtaat tttcctaaaa gaactctagt tttttcacca ccagaaagta
     3721 tgcttattgg cttatccata tcattgtcac taaatcctaa tccttttaga actcctttgg
     3781 cttcagattt atatccatat ccatttaaat ctgaaaataa ttctagttta tgtgaatatt
     3841 catccatgat tttttctata attggagagt ttgtttttgt actttcttca gatattttaa
     3901 cctccaattc tcttaaatag gattccattt ctatcaaatt tttaaaaact tctagcactt
     3961 cttccaaaat agtattatcc gaataaaaat tggtattttg ttctaaatat ccgatttcac
     4021 aatctttaga agtataaata tctccactat catatccata aattcctgaa attattttaa
     4081 ataaagtagt ttttccagta ccattaacac cgattatacc aattttatcg ccttcattta
     4141 ctgtaaaact aatattttct aatatagaat ctataccaaa acttttattt aagttattac
     4201 atgacaaaac aatcataaat ttctacctcc ttgaactatc taaaattata atgttttgat
     4261 ataatataat caaggttata tgtattagtt ttgaaaaaga aatattttat tgtgaatcaa
     4321 acaaaaaaga ggacactttt aggaggtgaa gatgttggga aataaaaata tatcaatggc
     4381 agttataaga aggctcccaa aatatcatag atatcttgga gacttattag atagggatat
     4441 acaaagaata tcttctaaag aattgagtga tataataggg tttaccgctt ctcaaataag
     4501 acaagattta aacaactttg gtggatttgg acaacaagga tatggttata atgtagaagc
     4561 tcttcatact gagataggta aaattcttgg gttggatcga ccatacaacg cagttcttgt
     4621 aggagcaggt aacttaggac aagctatagc caattatgca ggatttagaa aagctggatt
     4681 cgagataaaa gctttatttg atgcaaatcc tagaatgata ggtttaaaga taagagagtt
     4741 tgaagtatta gattcagata ctttagaaga ctttataaaa aacaataata tagatattgc
     4801 tgtattatgt atacctaaaa atggagcaca agaagttatt aatagagttg taaaagctgg
     4861 aatcaaaggt gtatggaatt ttgcaccttt agatttagaa gttccgaaag gtgttatagt
     4921 tgaaaatgta aacttaacag aaagtttatt taccttatcg tatttaatga aagaaggaaa
     4981 gtagacaact taaacatatt aataatgagc agttgtgaag agtattgaga aagtcagaag
     5041 atttaatatt aaatcctctg gctttttgaa atattaggat aaaaatttaa ataatgtatg
     5101 gaaataaaca atattaagga gagtggtttt tgtggaggaa ttatggaaaa aggcaattga
     5161 atttcatggt catgaatgtc cagggttagc tataggattt aaagcagcac ttgcagcaag
     5221 aaaatattta ggatgtaaac agtcaggaga tgaggaaatt gtatgtattt ctgaaaatga
     5281 tgcttgtgga atagatggta tacaagtcgt gctaagttgc acaatgggta aaggaaattt
     5341 actacttagg atgactggga aatcagtata tcatttcttt aatagggaaa atggaaaatc
     5401 tataagaata tatgtaaagt caaagccatt tcaaatggat agagaaggat acaaaaagta
     5461 cttattagaa agtgattttg aagaaatatt tgagattaca gatactaagt taagacttcc
     5521 tgaaaaagca agaatattta aatctcttaa atgtgaagtg tgtggtgaat ttgcatctga
     5581 gcaatatata aggattgaag aaggaaagaa agtatgtctt gattgcttta ataaatatga
     5641 aagattttat gaggagtatt aaaattataa attagattaa agttaaaagt attagatatg
     5701 tactaacagt attttaagat gaattaggta gcaacataat aaaatatata aataatatta
     5761 cttgaaccat acataattaa aaagaggctg attagtattt agaaaataaa atcagtctct
     5821 ttatgaataa cgaattatta ttctggttga ggtgcatcta caggacaagc tcctgcacaa
     5881 gaaccacagt ctatacaaga accagcatct ataacatatg cgtcgtctcc agctgatata
     5941 cagctaacag ggcattcagc ctcacaagca ccacagctta tacaagcgtc agtaatttta
     6001 tatgccattt tgaaatcctc ccttttatat gtattgtaat tcaataacat tataacaaat
     6061 attattgaat ataacaatta aattaattct acaacaaaaa ctttattatg atattcttgt
     6121 tttaaagttt tacaataaag tttaatctgc ttatattttt atttatttta aagaagttta
     6181 ttttaaaaat aaaattaaat ttaaaaagtt atgtattgat tatatttaaa aatgttatac
     6241 tctaaaaaag tatattttat tttaaaattt attaatacaa aggagattta ctatgaaaaa
     6301 taaattagtt tacatatgta ttatgagtct attgctattt gtagtaggat gttctaataa
     6361 tccaaataag gaaagtacca ttaaaaaaga ctctaagaat aaccaaagcc aagttaaaga
     6421 agaaaaaaga gaacctgtga aaaaagatat aactataagt ttcgctggtg atgtaactat
     6481 ggggaactat aaaggttcat catattatgg cagttttgac catgaattta aaagacaagg
     6541 agaagattac aattattttc ttaaaaatgt aaaacctgta tttgaaaaag atgacttaac
     6601 agtggttaat ttagaaggtc cacttactac agcgtcaaat gcaaaagaaa aaaaatttgc
     6661 atttaaagga aagcctggat atgtaaatat tttaaaatct ggaagtatag aggcagtaag
     6721 tattgcaaat aatcattcag aagattattt tgaagaagga atgaaagata cacagtttat
     6781 attggatgag aataaaataa attattttgg attagggaaa gatgctgttg tagatgtcaa
     6841 aggaattaaa gtgggtctat tagggtataa tggattatca actgaatata atgaaaaaaa
     6901 tttaaaacaa atggaagatg atattaagtc cttaaaaaag aaatcggatg ttgttgtagt
     6961 gtactttcat tgggggatag aattagatta ttatccagat aaaaaacaaa aagattttgc
     7021 tcattatgct atagacaagg gagcagattt agttatgggg tctcacccac atgttattca
     7081 aggaatagaa aagtataaaa ataagtatat agcttatagt ctagggaatt tttgctttgg
     7141 aggaaataag aatccttctg atacagatag ttatatatat caacaaacct ttactttttc
     7201 agataataaa ttaacttcaa ttaaagagcc aaatataata ccaacctcaa taacatctag
     7261 tagctccaga aataattatc aaccaaaagt attggatgga agtgagaagg atagagtttt
     7321 aaataaactt gaaaaaataa gcaaagattt aaataaataa aatgtaaaaa aaaataaata
     7381 tatatattta ttttaataat ttatattttg tttagggtat ggtaaatcgt ggtattaaat
     7441 aagtagattt taatagttaa attatagtca attataataa agctatttaa tctacttttt
     7501 taatataaaa atataagggg gaatttgtaa tggcttgtaa cacttgtaaa atgtgcgaaa
     7561 gtgcagacaa gaaattagaa agttttgtag cttcaaagga tgttgaaaca gcatttcata
     7621 gaacagaaga tcagaaagtt aagtgtggtt ttggacttca gggggtttgt tgtagactct
     7681 gttcaaatgg accatgtaga gtaactccta aatcaccaag aggtatatgt ggagcagatg
     7741 cagacactat agtagcaaga aactttttaa gagcagtagc atcaggagca gcttgttatt
     7801 tacatgtggt agaaaataca gctaagaatt taaaaaatgt tggaataact aaaggtgttg
     7861 ttaaaggaga aaaaacatta aatcaattag cagagatgtt tggaatagaa agtgatgaaa
     7921 agtatgataa atgtataaag gttgcagaca aagttatcaa cgatttgtat aaatcaagag
     7981 atgataaaat ggaattagta gaaaaaatag cttatgcacc tagagtgaaa aagtggaaag
     8041 agttaggtat aatgcctggt ggtgcaaagt cagaagtttt tgatgcaata gtaaaatcat
     8101 caacaaattt aaatagtgat ccagtagaca tgctagttaa ttgtttgaac ttaggtatat
     8161 caacaggatt atatggtctt acactaacta atcttttaaa tgatgttatg ttgggagaac
     8221 cagttataag aatggctcct gtaggattta atgtaataga cccagactac ataaatataa
     8281 tgataacagg ccatcaacat tcaacatttg caaatttcca agatagatta aaagatgaag
     8341 atgttataaa attagctcaa tctgttggtg caaaaggctt taagctagta ggatgtacat
     8401 gtgttggtca agatttacaa ttaagaggag aacattatca agaagtattt gctggacatg
     8461 caggtaacaa ttttacaagt gaggctgttt tatcaactgg agcaatagat atagttcttt
     8521 cagaatttaa ctgtactata ccagggcttg aaccaatagc agataaatat aaagtaaaaa
     8581 tggtttgttt agatgatgtt gctaaaaaag cgaatgcaga atacataggt ttagacagaa
     8641 gtaaattaga tgaacttagt aacactttaa tagaaaaagc attggagtct tataaggaga
     8701 gaagaggaag tattgagata gatataccaa aagaccatgg atttgaacaa tctttaactg
     8761 gtgtaagtga aaagaacctg aaagcgtttt tgggagattc atggaaacct cttattaatt
     8821 taattgcaga aggaaaaatt aaaggtgttg cagctgttgt aggatgttca aatatgacag
     8881 caggaggaca tgatgtaaat actgttgaac ttactaaaga attgattaaa aaagatataa
     8941 tagtactatc tgcaggatgt tctactggag gattagaaaa tgttggtctt atgtcacctg
     9001 gagcagaaga attagcagga gaaaacttaa aagaagtatg taagacttta ggaataccac
     9061 cagtattaaa ctttggacca tgtctagcta taggaagact agaaatagta gcaacagaat
     9121 tagcagctga acttggaatt gacctaccac aattaccatt agtgctttca gctccacaat
     9181 ggttggaaga acaagcatta gctgatggag cgtttggtct tgcccttggc ttacctcttc
     9241 atttagcttt acctccattt ataacaggag gaaaattggt gacagaagta ttaacagaaa
     9301 aattaaaaga cttaacagga ggacatgtta tagtgaatcc agaccctaaa tcttcagcta
     9361 atcaattaga agaaataata atagatagaa gaaaaaattt agggttaaag gatgtggaat
     9421 gcaatgcata gaatttttat aaataaagat ttatgtacag gatgtaagag ctgtgtacta
     9481 gcatgtatgc taaaacacaa taaagattat gacatgtaca ctcttgattt agaaaatata
     9541 gataatgata gtagagggca tatagaatta gatagtaaac ataataatcc agttccaatt
     9601 ctttgtagac actgtgatga acctgaatgt gtattagcat gtatgagtgg tgcaatgcat
     9661 aaagatagtg aaagtggaat cgtttcatat gatgaagaga aatgtggttc atgttatatg
     9721 tgtgtaatgt cttgtccata tggattatta aagccagatg atagaagcaa gcaaaacata
     9781 cttaaatgtg atttatgtaa agatgaggag tatcctagat gtgtagctaa ttgcccaagt
     9841 ggagcaattg aattacagaa ggaggaaaat gatgaattat gtagtgttag gggctagtgc
     9901 agctggtata aatgcagtaa aaacattaag agaattagat aaagattcaa atatagtagt
     9961 aatttctaaa gatgaaaacg tatattcaag atgtatgttg catcacgtta tatctgaaca
    10021 cagaacttta aaacaaataa attttgttga tgaagatttt atggagcaaa ataatgtaaa
    10081 atggattgca ggtaagactg ttaaaggtat agacataaat aaaaaggtag ttcaaacaga
    10141 agacattact gtaaattatg ataaactttt aatagctaca ggtgcatctt ctgctattcc
    10201 tcctataaaa aatttaagag atggaaattt tgtatattct gtgagaaaca tagatgatat
    10261 ttataaaata aaagaaaaag cacaaaattc taaaaatgta gttataatag gtgctggttt
    10321 agttggtata gatgcattag tgggattatt taaatatgag cagttaaata tttctgtagc
    10381 ttttatggaa aagtacattt tagatagaca attggatgaa tatacagcat cagtgtatga
    10441 aaataagttc aaagaaaaag gtgtaaaatt ttacccaagt gcatcaatac aagaaattgt
    10501 attggataac agtaaaaatg ttacaggtgt tgcattttca aatggtgaag ttttagatgc
    10561 agacatggta attgtagcta caggggttaa accaaatgct gactttttag atgggacagg
    10621 aatagaatat gatagaggta taataataga tgatatgtgc caaacaacac aaaaagacat
    10681 atatgcagca ggagatgttg taggtaaaaa tgctatttgg cctctggcag taaagcaagg
    10741 aattgtagct gcctataata tggttggaaa agataagaag atagaagatg aatttgcttt
    10801 taaaaatagt atgaatttca tggatatacc aactatttct ataggaatga atacacctgt
    10861 agatgatagc tataaagtat taaccagaca tggaattaat gattataaga agtttgtttt
    10921 taaagataat gtaatctacg gagcagttat acaaggagat atttcatatg taggtgtatt
    10981 aacttattta ataaaaaata aagttgaaat atatgattta gaaaatagga tatttgatgt
    11041 atgttatgca gactttttta atataaaaga aaatggagaa ttttgttata gtgtatagta
    11101 tatacgtgga gtaaggttta taaatacagt aaatcaataa atagagttat tctaaaataa
    11161 gaaaaatgac aaatc
//
LOCUS       DAELXX010000118        11113 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.119, whole genome shotgun sequence.
ACCESSION   DAELXX010000118 DAELXX010000000
VERSION     DAELXX010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11113
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.119"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            161..925
                     /locus_tag="KPH29_003080"
     CDS             161..925
                     /locus_tag="KPH29_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaP domain-containing protein"
                     /protein_id="HBG1468653.1"
                     /translation="MQIIDININNIDSRHICCSISEKKGENCVFSKKAWMKNNFDKGL
                     AFKRFDVRGKAFIEYIPAENAWCPITADNYIFINCFWISGKFKGQGYSNLLLQECVDD
                     AKSKGKYGLVVLSSSKKMPFLSDAKYLKYKGFCLADTSEPNYELLYFPFTDNAPIPCF
                     KNCVKKRKLNENGIVLYYSNQCPHTEKYAPILKKIANSRGIDVNLIKFETAKEAQNAP
                     APFTTYSLFFNGEFVTNEILSEKKIEKFLDTNIKKI"
     misc_binding    1148..1361
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1615..2649
                     /locus_tag="KPH29_003081"
     CDS             1615..2649
                     /locus_tag="KPH29_003081"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBG1468654.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLMNHDKVEVTAIGSNSYVGKNI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEVL
                     PLDKVASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGAAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     gene            2691..3908
                     /gene="argJ"
                     /locus_tag="KPH29_003082"
     CDS             2691..3908
                     /gene="argJ"
                     /locus_tag="KPH29_003082"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HBG1468655.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKENDVLVASTGVIGKPLNINLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEEHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            3923..4783
                     /gene="argB"
                     /locus_tag="KPH29_003083"
     CDS             3923..4783
                     /gene="argB"
                     /locus_tag="KPH29_003083"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBG1468656.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGMDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKENE"
     gene            4813..6000
                     /locus_tag="KPH29_003084"
     CDS             4813..6000
                     /locus_tag="KPH29_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HBG1468657.1"
                     /translation="MNNNNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLYSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYIERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLEIVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVVLKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            6109..7131
                     /gene="argF"
                     /locus_tag="KPH29_003085"
     CDS             6109..7131
                     /gene="argF"
                     /locus_tag="KPH29_003085"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBG1468658.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAYVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQKIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLI"
     gene            7258..7875
                     /locus_tag="KPH29_003086"
     CDS             7258..7875
                     /locus_tag="KPH29_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HBG1468659.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIDSFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSVKETYVTK
                     TVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
     gene            complement(8095..8793)
                     /locus_tag="KPH29_003087"
     CDS             complement(8095..8793)
                     /locus_tag="KPH29_003087"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBG1468660.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHELMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNNL
                     IQNIKMDKENPHNIDIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
     gene            complement(8955..10172)
                     /locus_tag="KPH29_003088"
     CDS             complement(8955..10172)
                     /locus_tag="KPH29_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HBG1468661.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWME
                     GLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNRSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTADLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            complement(10363..10776)
                     /locus_tag="KPH29_003089"
     CDS             complement(10363..10776)
                     /locus_tag="KPH29_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG1468662.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKTEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYYRKIL"
BASE COUNT         4370 a         1206 c         1827 g         3710 t
ORIGIN      
        1 tatttaatta tatatgcaat ttgataattc aaataatcta aaaataatat tatgtaaaat
       61 aaaaatatat atgacatgat gttgttgtat tcaaaatatt aagataaaaa tatatagaat
      121 atatttaaaa attattatgt aattagaaaa ggagataagt atgcagatta tagatataaa
      181 tataaataat attgacagta gacatatatg ctgttcaatt tctgaaaaaa aaggagaaaa
      241 ttgtgttttt tctaaaaaag cttggatgaa aaataatttt gataaaggtc tagcctttaa
      301 acgatttgat gtaagaggta aagcttttat agaatatatt ccagcagaaa atgcatggtg
      361 tcctattact gcggacaatt atatatttat taactgtttt tggatatctg gaaaatttaa
      421 gggtcaagga tattctaatc tattgctaca agagtgtgtt gatgatgcaa aatcaaaagg
      481 taaatatgga ttagttgtgc tttcgtctag taaaaaaatg ccttttttat ctgatgctaa
      541 atatctaaaa tataagggct tttgtttagc agatacatca gaaccaaatt atgagctatt
      601 atattttccc tttactgata atgctcctat tccatgtttt aagaattgtg taaaaaaaag
      661 aaaattaaat gagaacggta tagttttata ttacagtaat cagtgtccac atacagaaaa
      721 atatgctcct attttgaaaa aaattgcaaa ttctagaggt attgatgtaa atcttataaa
      781 attcgaaaca gcaaaagaag cacaaaatgc tcctgcacca tttactacat acagtctttt
      841 ttttaatggt gaatttgtaa ccaacgagat actttctgag aaaaagattg agaaattttt
      901 agataccaac attaaaaaaa tttaactaaa aatagatgta aatattgtat tttgagatta
      961 cattatttta taatccaata tattttggaa atttcttaag ttgatgaatg ataatataaa
     1021 gattataact caatttagat acaaaatgct ggaaattaac catatagaaa atatttccaa
     1081 aaatttaaaa attatattga caaagttaaa aaaataaact atcatataaa acaataagaa
     1141 aataaattga aatcctgtga aaaagagagt agcataaagc tagttgatag agagctggga
     1201 atggtggaag cctagtaacg aattttattg tgaatagagc tttggaggag attaccagaa
     1261 gtaatagtat ggtaattggt gaatctcgcc ttaagagatt agagtgggta agattatctt
     1321 atcaattaga gtggtaacgc gggtgtatat tcgtctcttg gtctttgacc aagaggcttt
     1381 tttaatttta aatgtacatt ttgtagatac tttttaattt gagttttaat gattattgta
     1441 attttaccaa tgagagtggt accgcggaaa atcgcctctt agtatttata atgctaaggg
     1501 gttttttgtt ttaattagaa tattttaatc tacatattat caaatgatta tcatatagta
     1561 aagtggaaag atagaatttt aatactattt gaatataaaa atggagggaa caaaatgaat
     1621 aaaataaaag ttggtattat tggcgcaact ggatatgtcg gggcagagtt gattagatta
     1681 ttaatgaatc atgataaagt cgaagtgaca gcaataggtt caaattcgta tgttggaaaa
     1741 aatatagttg atatatatcc aagtatagga tataaaaata acatgatatg tatagaaaat
     1801 gaaaaagtta tagatatgtg tgatgttgtg tttacagccc ttccacatgg agttagtgaa
     1861 aagtttgtaa taaaagcaat taaatcgaaa aagaaagtta tagatttagg tgctgacttt
     1921 agaataaaag atgaagaagt atatagtaaa tggtatggag tatcttttat agataaaatc
     1981 ttacataaaa aagctgtata tggattaagt gaaatataca aagaagatat aaaagatgca
     2041 gatataattg caaatcctgg atgttatcca actagtattt ctttaccatt gatgccatta
     2101 ctaagttcta aattgataaa aaataataat ataataattg attctaaatc aggtcttaca
     2161 ggtgctggta gagaattatc gattagtagt cactttacgg aggttaatga aaatatcact
     2221 gcatataaaa tagggaaaca cagacatact ccagaaatag aacaaaattt aagtgaatcc
     2281 tgcaaagaaa aagtaagtgt agtttttaca ccaaacttaa taccagtaaa taggggaatc
     2341 ttatcaacta tatattgtac aaaagaagat aatatttcta taaatgatat acatagaaaa
     2401 ttaactgatt attatgaatt taaagaattt atagaagttc tcccattaga taaggtagct
     2461 tctttaaaga atgttaggtt ctcaaataaa tgtgtcatat cattacatga aaatggagat
     2521 acattgataa tctgttcagc tatagataat atgattaagg gagcagcagg tcaagctata
     2581 caaaacatga atataatatt tggtattgaa gaaaatacag gtttaaaaaa tattgcacca
     2641 tcattttagt aatgaaaaaa tactaaactc atataattag gaggataaat atggagatta
     2701 taaagggtgg agttacagtt tcagaaggat tttttgcttc agggattcac tgtggcttga
     2761 gaaaaaataa agaaaaaaga gatttagctt tggtttattc agatgtttta tgtgatgcag
     2821 cagcagtagt aactcaaaat aaagtaaagg gcaatcctgt ttatgttaca caggaacaca
     2881 ttaaaaatgg aaaagcacaa gctatcatag taaatagtgc taatgctaat acctttaatg
     2941 gaaaagaagg acttataaat gcttataaaa tggctaaatt cactagtgat aaattaaata
     3001 ttaaagaaaa tgatgttttg gttgcatcta caggtgtcat aggaaagcca ttaaatataa
     3061 atttaataga agaaaatatg gatgaattag tgaataatct aagtaaacaa ggtcatattg
     3121 gagcaagaga agctattatg acaactgata ttataaaaaa agaaatagca gtagcaataa
     3181 tgtatggaga taaaaaaata actattggag gtatggcaaa aggttctggc atgatacatc
     3241 ctaatatggc aacaacgtta ggatttataa ctacagatgc aaatattgat ggagttttgc
     3301 taaaagaagc tttgaaaatt gctgttgata aaagttttaa tagagttagt gtagatggag
     3361 atacaagtac taatgatatg gtgatgattc tagcaaatgg aaaagcaaaa aatgatagaa
     3421 taaacaaaaa agatgaacat tatcaagtat ttttgagtgc acttacatat gtctgtatag
     3481 aacttgctaa acttgttgca aaagatggag aaggatgtac aaaactaatt gaatgttata
     3541 taaatggagc attaagtgaa gaacatgcag taaaattagc aaaaacagtt atatcttctt
     3601 cattagtaaa gacagctgta tttggagctg acgcaaattg gggtagaata ctgtgtgctt
     3661 taggttatgc aggtgaagaa atagatatgg aaaaagttga tattattttt gagagtatga
     3721 aaggatatat tgaagtatgt aaaaatggaa atggattaga ttttaatgag gaaaaagcaa
     3781 aaaaaatcct tgaagatgac gaaataagta tattagtaga tttaaatatg ggcaatgcca
     3841 gaggaaatgc atggggatgt gatttaagct atgattatgt aagaataaat ggaagttata
     3901 gaaattagga ggatatactt taatgattaa tatagaaaaa gcaaatacac ttatagaagc
     3961 tttaccatat atagaaaaac atcagggaaa gactattgta gttaagtatg gtggaagtgc
     4021 aatgaaaaaa gatggtctaa aagaatctgt tatggaagac cttgtattaa tgagttatgt
     4081 tggaatcaat atagttttag tacatggtgg aggggcagaa atcaataaga tgctagcaaa
     4141 agtggatata gaaagtaaat ttgtaaatgg tcttagatat acagatgaag agaccatgga
     4201 aattgtaaaa atggtacttg ctggaaaggt taataaagac ctagtaaata aaatacatac
     4261 aaaaggtgga aaagcagtag ggctttgtgg aatggataat aatatgattt tatgtgaccc
     4321 atataaaaat tatgaacttg gatttgtagg agaaatcaaa aaagtaaatg ttgagcttat
     4381 agaatcttgt ctaaagtcag gttatatttc agtaattgca actataggtg ttggagatga
     4441 tggagagact tataatataa atggtgacac agcagcttct gctatagcta aagagttaaa
     4501 tgcagataaa ttgatacttc ttacagatgt ccctggtttg ttaagagagc cagatgaaga
     4561 aaaatcatta ataacagaag ttatattaga ggatgttgat aaattatttg aagaaggtat
     4621 aattactgga ggtatgatac ctaaaataga gggatgcgta gatgccttaa ataacggagt
     4681 aaatagggta catattttgg atggaagagt tccacattct ataataacag agctatttac
     4741 tgatagtgga ataggtacat taataagaaa agaaaatgaa taaaattata agtttaagag
     4801 gggagaataa gaatgaataa caacaataca atttcaaaat ggaatgaata ttttatagat
     4861 acatataacc aacccaattt tgtcatagat tatggtgaag gaagttgttt ttttgatact
     4921 aatggcaata aatacataga ttttacaagt ggatatggag tatcttctct tgggtatagt
     4981 aatagtaatt taaagaatgc tctaaaagag caagtagata aattattaca tacttcaaat
     5041 ttatatttta atgagccagt gttgtattca ggagagaaaa taattaattc aagtggaatg
     5101 gctaaagtat atttttgtaa ttctggaaca gaagcaaatg aaacagcatt caaaatagca
     5161 agaaagtata gtagtgacaa atatggtaat ggtagaggaa ctatcataag tttaaaagac
     5221 tcttttcatg gtagaacaat gatgtcttta atggcaactg gtatggacaa ataccataag
     5281 tatttttatc ctcttcccga aggttttaaa tatatagaaa gaaataatat tgaagattta
     5341 aaaaataatc ttgattccac agtttgtgca ataattcttg aagcgattca aggcgaaggt
     5401 ggagttaatg ttcttgaaaa agattatgtt ttggaaatag taaaaatatg tcaagaaaag
     5461 gatattgtag tgatttttga tgaagtccaa tgcggtatag gaagaactgg taaacttttt
     5521 ggctatgaat attttgatgt aaaaccagat atagttaccg tagcaaaagg gttgggagca
     5581 ggaataccag taggaggagt attggtaaat aaaaaacttt caaaagtact aggaaaagga
     5641 gaccaaggta caacttttgg aggtaattta ttagctatgg tggcagcttc agttgtatta
     5701 gatgagatat ctaaagatgg tttttataat gaagtattag aaaaaggaaa ttatataaga
     5761 aaatctattg aaagttttaa taataaagtt gtattaaaga caaaaggtat tggtctaatg
     5821 ataggaattg aaactaatat tgaatctagt attatagaag agaaagctag aaaaaaagga
     5881 ttgcttattt tgactgctgg aaaaaatgta ttgagatttt taccaccatt aacaatcagt
     5941 tataaggaaa tagatgaggc tcttgaaatc ctaaaagata tattactaga gattaactaa
     6001 atattcaaaa aatagttaat taaagataaa aattagaagt aaataaaaaa gtaaagtgct
     6061 aacaagttta attgaattta agaagtttat aagaaggggc gaattaatat gaataatata
     6121 agtttaaaag gtaagagttt tttgacatta aaggatttta caaaagaaga gattagatat
     6181 ttattaactt tatcaagtta tttaaaaact aagaaaaaag ttggcataaa aggcgatttg
     6241 ctacaaggaa aaaatatagc tttattattt gagaaaacat caacaagaac tagatgttca
     6301 tttgaagtag cagctcatga tgaaggagca tatgtaactt atctaggtcc aaatgattgt
     6361 catatgggta aaaaagagtc tgtatcagat acagcaaaag ttctaggaag attctatgat
     6421 ggaatagaat ttagaggctt taaacaagaa acagttgaaa gtctagcaaa atattctggt
     6481 gtacctgttt ggaatggact tacagatgaa tatcatccaa cacaaatact ggcagatttt
     6541 ttaacagtaa ttgaaaatgt agataaagat ttgaataaag ttaaatttgt atatgttggt
     6601 gatgctagaa ataatatggg aaactcactg atgataggat gtgctaaaat gggaatacat
     6661 ttcgtagcac tagcacctaa ggaactttgg ccaaatgaag aattggttaa ggaaatgaag
     6721 gaaatagcca agaaatccaa aggagatata atgttgactg aaaatattga tgatgttaaa
     6781 ggtgcagatg ttatatatac agatgtatgg gtttctatgg gagaagagga gcaatatgaa
     6841 gcaagaataa atcaacttaa agattatcaa gtaaatatgg atatgataaa gaaaactgaa
     6901 aatgaaaatg ttatattttt acactgtctt ccagcatttc atgatttaga aactaaggtt
     6961 gctcagaaga tttatgaaaa gttcggatta tcagaattag aggttacaaa cgaggtattt
     7021 aattcaaaat attcaaaggt atttgaagaa gctgaaaata gaatgcatac aataaaagct
     7081 gttatggttt ctactatgac tgatgtaaat atgaacaata atttaatata gatatgtttt
     7141 aatatagact ttactttaat gaaaatttat aaaatatttt aaatatatta ttcaaaaact
     7201 ctcattatat gttaaaataa cttatgtatc aacaaattaa gagggaggat ttgagatatg
     7261 aaaaaaatca tattagcttt aacaactttt ttattagcaa tatctttaac agcatgttca
     7321 agtgcaaaac cagaagatac aattgatagt ttttttaaca gtgcgaaaaa gtttgacttt
     7381 gaaggcatga ataaggtaat ggaaaataat gacgaaaaat ataaggatat attaaaggaa
     7441 ttagatacaa aagacccaaa tgcccaatat gttttagatt atctaaaaca gaatgcatct
     7501 aaaatcacgt atacaataaa agacagcgag gttaaaggtg ataaggctac aatcaaagta
     7561 gaatgtaaat ttatagattc aacaccatta cttcaagaaa tagtagctga ggcatttaca
     7621 aagatgattg gtatgagttt ttcaggacaa gacttaactg atgagaaaac aactgaaatg
     7681 ttagtatcaa ttatgaaaga aaaacaaaaa agcgttaaag agacttatgt tacaaaaact
     7741 gtagagtttg aatgttctaa aaaggataat aaatggataa taagttcagt aaatgatgca
     7801 gtagctgatg tattgttatc taatcttgtt acagcaggac aagaattttc taactctatg
     7861 aatacacttg aataatattg tttaggtgta gtaaagtaat aaatatatga ttatttttat
     7921 aaataaacat ataaatattt atgaaggata ccatttttgg tatccttttt attaatataa
     7981 tgaaatacat ggttaatttc tatattgtca agaaactatg atatatgtta taagtctata
     8041 ctatttaaaa taaacttata acatatattt tttataatat agtgcatgaa aaatttattt
     8101 aactaatttt agatatttat tgacaactga ttcagccaag cattttgatg aatcaacaat
     8161 atttattgaa ggatatgatt tttctaaaac tacatttaaa tcagtacaag caattactat
     8221 attttctata gattcatttt taacatcttc gataagttta ttccaaatat caatattgtg
     8281 tggattttct ttatccattt ttatattttg aataagattg tttaatttta tttgccattc
     8341 atctttaaat ataaattcat gtccattatg tattatacca ttctggtaaa ttgtagactc
     8401 aaaagttgaa ctagtcgaaa aaagagttac tttttgtgag atagtaggta attttttaac
     8461 tgtttcttct acaatattta atattggtat attaatagac tcttttaatt ctttaaagta
     8521 tatatgtgct gaattgcatg gcattgcaat aaaatttaca ccagttgatt caagcttttg
     8581 taatccatca attatagttt ctttcataag ttcatgattt attggacggt caatataaaa
     8641 tggggtaggt aatgaatata tcatcatctt tggaaattca tcatcatatt ttgctccata
     8701 ttgagattga cactcatcaa taattaaatc aacaaaaggg gctgttgacc ttggacccat
     8761 tccagccaaa ataccaatta atttttcatt cataaaaatt atctccttta tatataaata
     8821 tatttatgta taaaataaaa atctataaaa gcataaaatt atattttata atagattata
     8881 ttgtacactt gtataatata aatataaagt gattttttag attatatatt ttaatatact
     8941 ttcttatata aaaattattt taaatcaaga ttatctagta tttttaaaaa tatttttaca
     9001 gcagtatcca agtcatctac agaaacactt tcattaggat tatgacttac gcccccagca
     9061 catcttataa ataccatacc aatatcagtt atattatcca tttcctgagc atcatggcca
     9121 gcaccactat aaatataaaa aggattaaga tttaaatcta taaaactttt ttctatgatt
     9181 tttgttattt tattagaaca tggtacagga acattttcaa aagcaagttc acttgtataa
     9241 ttaagttttc tattttcaca tatatgtgat atttcattaa atatcttttc tacagaatta
     9301 tcaagaattt cttgtgaaca agagcgtaca tcgagtgtaa attcagctct ttcaggtacc
     9361 acatttgatg aagatggata gaaattcatc tttcctacag tagcaaccaa atcagcagtc
     9421 gttttagcta ctttttcgat agctaataca cattcacagg cacaacatcc agcatcaagt
     9481 ctcatattca ttggaagtgt accagcatgt ccagacttac catttactga tatcttaaaa
     9541 cttttaaatc ctgtaatact tgatacaatt ccagcaggaa gagattcatt ttctaaaaca
     9601 ggaccctgct cgatatgaaa ttctaaatat gcatctacat cttttctagg atgcaaattt
     9661 gttaagtttt ttgtattaaa tccaaacttt gttacagctt cttcaagtgt aattccattt
     9721 tcatcaacag atttaagaag ttcctcttta aaagtaccag caaaaacttt acttcccatt
     9781 aaagaagttt caaatctagt tccttcctct tcagcgaaag caattagctt aatattgaaa
     9841 ggataagacc tattattgtg ttttgcttct tcaagaccaa ctagaggcac tattatacct
     9901 aacataccat caaatatacc tccacacttt acactatctt gatgagatgc aactactaat
     9961 gtgggagcat ttggaaagct tgatttatat gtacctataa cgtttccaat gtcatcaatt
    10021 tcaatatcaa gatttaaacc ttccatccat gaactaatta aatcaataga ttttttatgt
    10081 tcttgagtca agataagtct tgtaagacca tttgttgttg aacttatttt tcctaatgtt
    10141 tgtagataat ccatacattt ttttcctaac atatttacct cctaaaaata taatttttat
    10201 ttaagctaag attagctttt atacttataa tttttgaact ttcaaataat tttgtatgta
    10261 tagtaaaata ctattttagt attaataatg atataaaaac ataaagtctg tagacgaaga
    10321 gcagaagttt taagttaaaa atataaaata tgatttttat tattatagta tttttctata
    10381 ataatatctc tttccagtta gaggataatt ttcaagtgaa aatactttat ggtatccaaa
    10441 tttctcataa aatttaggtg cttgaaaact gaatgtatct aaaaaaacat atttacaacc
    10501 tcgttcttta gcaatttttt cagtcttata taaaagttga cttccagtac ctttatatct
    10561 aagcttctca ctaacccata agtattttat agaaagccaa ttaccatgag taacacctat
    10621 aaaacctgca acaattttac ctgctttatc tcgaatatat attcctaaat ccactgggtt
    10681 tttatcttca agtctagcaa gattatattt taataatcct tgaaaaatag tatcttcatc
    10741 ttgtttttct attttttctg taatttctaa ttccatattt gaactttctc cttgtataat
    10801 cttttgtata ttggtatttt ttaataaaat cagtagaaag aatatagttt aacattattc
    10861 taataggcat ttatgctaat gtcaaactaa taatacttat tcttaatttt atattgttga
    10921 tattaaattt tttaaaataa aaatgtagtt taagttatat aaaaagaaat catcaataaa
    10981 tttagtaaac ctaaattgtt gaatattaaa ataagttaat ttaatacata aaaactattt
    11041 tagatgttat tctattgcta tgttcaataa tatcattagt agttcaattt ataaaatgaa
    11101 taaattatat aaa
//
LOCUS       DAELXX010000119        11050 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.120, whole genome shotgun sequence.
ACCESSION   DAELXX010000119 DAELXX010000000
VERSION     DAELXX010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11050
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.120"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(57..908)
                     /gene="gatY"
                     /locus_tag="KPH29_003090"
     CDS             complement(57..908)
                     /gene="gatY"
                     /locus_tag="KPH29_003090"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBG1468663.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVKIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            complement(957..2126)
                     /locus_tag="KPH29_003091"
     CDS             complement(957..2126)
                     /locus_tag="KPH29_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG1468664.1"
                     /translation="MKMFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKL
                     NRFIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNP
                     YDYLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPQGELAKMTKND
                     ENNYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIIEMGRNVID
                     RKDEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPK
                     SFIDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFK
                     EKYNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            2588..3505
                     /locus_tag="KPH29_003092"
     CDS             2588..3505
                     /locus_tag="KPH29_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBG1468665.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     ICSDYRIMPFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVNQ
                     GEELIELSKKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVRFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKHFSVNTSMHRDSG
                     TKLEQVEILSKGKIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAINGEECIKAQRLLEKIINSVK"
     gene            complement(3634..4563)
                     /gene="pfkB"
                     /locus_tag="KPH29_003093"
     CDS             complement(3634..4563)
                     /gene="pfkB"
                     /locus_tag="KPH29_003093"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBG1468666.1"
                     /translation="MITVITFNPSIDRMYKVNNINIGEVQRVVSKNATAGGKGLNVAK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGINIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEYNVYDVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLGEIDKYQDLVEVKKLS"
     gene            4832..5557
                     /locus_tag="KPH29_003094"
     CDS             4832..5557
                     /locus_tag="KPH29_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG1468667.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRIQGKKPGTK
                     VLSFEVVESTKYFSEKLGITPGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            5640..6794
                     /gene="nagA"
                     /locus_tag="KPH29_003095"
     CDS             5640..6794
                     /gene="nagA"
                     /locus_tag="KPH29_003095"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBG1468668.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQERPNITDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKDPDINILKLWQRKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFSVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNIATLEDAIKM
                     ATYVPALSCNIDNVCGSIHQGMDADFIVTDKNFNIYETYISGICMYKVNK"
     gene            complement(7154..8368)
                     /locus_tag="KPH29_003096"
                     /pseudo
     CDS             complement(7154..8368)
                     /locus_tag="KPH29_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(8610..9341)
                     /locus_tag="KPH29_003097"
     CDS             complement(8610..9341)
                     /locus_tag="KPH29_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468669.1"
                     /translation="MLNKKNVSLIVLVIVLSLFTAIFVFNNRNTNDKSKEIAKNNEEI
                     VPKNLEALSIEQLTKIRDKAIDKNLEGLREFGEDSVKQRKDSILNFEFGLVAFVNKNY
                     SNLTEKEKLIRQINIVNYENSINDIMDPSGSFFELANQSYEINEFMLKNKKNYKEWSY
                     EYIEFLIDHLDELCKFVDFDIKDVIDIIDPTIKTDLSEEQKKSLNDKLKKVSSSDTLS
                     EDIKKEIKNWENNLNSLNMNKNNSN"
     gene            complement(9675..10928)
                     /locus_tag="KPH29_003098"
     CDS             complement(9675..10928)
                     /locus_tag="KPH29_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HBG1468670.1"
                     /translation="MLNKKNISSIIMTMILSLSTTVFVFADENTNVKGQDVTKYNEEM
                     VSKNLEDLSIEQLIDIRDKAIDKNLEGLRKFGEDSVKERKDSILNFEFGLVAFVNKNY
                     SNLTEKEKLIRQINIVEYENSINDIMDPSGDFFKVTNQSYQINEFVLKNGKNYKEWSY
                     EYVEFLIEHLEDLCNFVDFDAKYVIEMVDPSIKPDLSDNQKKSLNNELIKMYSSNTLS
                     TNIKDEIENWENNLNINEKNSNEPLPRVSPSSYRQQALAYAKKHGYTNGYYGSTNRKN
                     NAPSPYYNFQKDGYGDCANFVSQCLHEAGVTFWTNNNPWYYYSTSNRSPSWAGAREFK
                     IHWMNRIAYQTLTVSNSLSFLRPATPVSILNSSGVATHTLISTDKHSNGYNFSYAAHS
                     DEGARTNLLKKLEGKKISYYKVYWE"
BASE COUNT         3765 a         1672 c         1316 g         4297 t
ORIGIN      
        1 ttttcatata acaaatatcc taaacttatc taaaataaat ttaggatatt ttatttttat
       61 actctattta cagacccaca cactttaatc ttttctttta caaaatcctt cactgattga
      121 agccctgcta atccatactt tttagggtct ataacctcag aatcttcatc taaaactttt
      181 ttaacccctt tactatatgc aattcttaat tcagttgcat agttaacttt tgcaatacct
      241 ctttttatag cttcttttat aatatcatca tgtattcctg aacctccatg caatactaaa
      301 ggtattgaaa ctacattttt aatttctgat aacctatcta aatctatttt aggttcaccc
      361 ttatacaatc catgtgcagt acctatagca atagccaatg aagatattcc tgttctatcg
      421 acaaactctt ttgcctcttt tggatttgta taatcattac tattcccgcc atctaaatca
      481 tcttcttttc caccaacttt cccaagttct gcttccactg gtataagaga aggactacat
      541 gcttttacta ctgatttaga tatagatata ttattttcaa agctttcatg agaaccatct
      601 atcattattg atgtatatcc acttcttaaa gctttcattg caaggtcaaa acttgaacca
      661 tggtccagat gcattgctac aggtatcttt actctttctg cagcaacctt tgcatttgct
      721 agataataat caagacctgc atattttaca gtagatggag tagtttgtaa tattactgga
      781 gagtttaact cctcagctgc ttctataaca gcaattacca tttccatatt ttctacatta
      841 aatgctccta ctgcataccc ctcatcttgt gcctttttaa gcattgatgt tgttgttact
      901 aatgccacat tgataccctc ccaaattatt tagttataat attttaatct tatcttttaa
      961 tattcatata ttgttacacc ttttactact ctatttactg ttcctgtagc tgatggcgta
     1021 tctggcttat ttccaacctt taccgataca aacaatgcta tagtttgtgc aaacaatatg
     1081 tatggcattg caagatacac atctggtaat ttattatact tttctttaaa ctcaaacttt
     1141 gttcctgaga agttattttc attttctact gacactgcac aagtgaattt agcaatttta
     1201 tcccttttta tctcctctaa aacatctaaa tcatattttc ttgtatataa acagttagat
     1261 acaaattcaa atacaagtgt attttcatca ataaatgatt ttggtccgtg tctaaacccc
     1321 ataggagaat catacaccgt agatattttt cctgctgtaa gttctaaaag ttttagttgt
     1381 gcttcttgtg tcaaaccacc taatcctcct gaacctaaat atacaactct atcgaaatct
     1441 ttatttatta actcatgtat ttcatctttt ctatctataa catttcttcc catttcaatt
     1501 attgcattta tatatgactt ttctacatca tcttctaaac tatcgaatat taacatagca
     1561 gataacatca tacacgaaaa acttcctgtc atagcaaagc cttcatcatt tgattttgat
     1621 ggcattaata acaagtaatt attttcgtcg tttttagtca ttttagctag ttctccttgc
     1681 ggattacaag ttatagctaa atgataaaaa ttatctacaa tcttattccc taaatttaaa
     1741 gcagccaaac tttcaggact atttccactt cttgcaaatg aaattaataa tgttggtata
     1801 tcttttttta agtaatcata tgggtttgaa actatatccg tagtaggtat tgcttcaaat
     1861 atgtattttc ttatgtcatt tttattctta aggtaaggaa gtattgaatt tcctatgtaa
     1921 gctgaagttc cagcacctgt aaatataact ctaaactgcc catgattatt acttattgta
     1981 tctatgaatc tatttaattt ttctttgtta gatttatata tttcataagt ctctaaccat
     2041 aactcaggtt gttgttttat ttcattagtt gttattattg cacctaaatc ttttagtttt
     2101 tgttcttcta atttaaacat tttcattctc ctttcatact tgtaacgttt acatttggta
     2161 atgaggttat aaccacttta aactataaaa aaatatactc ttttataatt ttttgcttag
     2221 attttttagt actacttaaa ctcactataa aatatacgct atagatttta attaatttta
     2281 tagtttatct ttaaaaattt aataaaatca tactttagta tttttatttt acgataaaaa
     2341 cttgttcaga ccaaagtggt aattaccact aatccaaata taaattaatt tgaatttttt
     2401 gtcaatacat aaaatattta tatcaatcaa ttttatagat atcctacaaa attactttgt
     2461 caatatttct tatttcgtta atttacttat ataagcttta aatttatttt ttgatacaaa
     2521 gtttacaaat ttatgtgtct gaattataat caattctgta aatataaaaa ccttaggaga
     2581 aagtattatg aaaaatataa aaatgggaat gataggtctt ggctccatag ctcaaaaggc
     2641 atatttacct atattaacta agtctgagag atttgagttt gtaggagctt ttactccaaa
     2701 taaagttaaa agggaaaaaa tctgtagtga ttatagaatt atgccattcg attctattga
     2761 atccctagct aaaaaatgtg attgtatttt tctacatagc tctacagaaa cacactacga
     2821 aattataaaa atattattaa atttaggtgt tcatgtatat gtagacaaac ctcttgcttc
     2881 aacagtaaat caaggagaag aacttatcga gttaagtaag aaaaaaaact taaatttaat
     2941 ggttggattc aaccgtagat tctgtcctat gtacaaagaa attaaaaata atgctacaga
     3001 aatcgtatct atcaatatat gtaaacatgg attaaattcc ttaagaaatg ttagattcga
     3061 cagtaccctt atagatgact atatacatgt tattgacact gctttatggt tagcaaatga
     3121 agatgttgaa attagcggag aagatttatt tttaacagac aataagaatc ttatatttgt
     3181 aagtcataaa cttaaaggta aacatttctc tgtaaacacc tctatgcata gagattctgg
     3241 aacaaagtta gaacaagttg aaattctttc aaaaggtaag attcaaagag ttaagaatct
     3301 taatgtactg gaaatagaag aaggtggaaa cctaacttta aaacaaagtg gtgcatgggt
     3361 aaatatatta aaacaaaagg gttttgagga tatatcaaat cattttattg attgtataga
     3421 aaataatata aaaccagcta ttaatggaga agagtgtatc aaggctcaaa gattactaga
     3481 gaaaattata aattcagtaa aatagatatt tattataaaa ataggttaaa aatgaatttt
     3541 tctaacctat ttttatataa gactatatct tttatattaa aattaaaatc tattacaaca
     3601 acattaaatt tattatattc ccattaaatt tatttaactt aactttttta cttcaactaa
     3661 atcttgatat ttatcaattt ctcccaaatc tacaaatcca gtttccatat ttaaagcatt
     3721 acttataccg caagcatttg caaatttaag taaattttct attttataac cccttaaaat
     3781 acctacacta aatccagcca ctgtgctatc tccactacct acagtattta tacactttac
     3841 aactggtact tttacatcat atacattata ttcgtttaaa tacaccatac catcttttcc
     3901 taaagatata catacattct ctgcacccat ttcaataagt ttttttcctg acaagataat
     3961 ttcctctctt gattctatat ttattcctaa taaatgtctt atttcatcta tatttggctt
     4021 tattaaatat ggttttgatt ttaaagctat ttttaatgct tctcctgatg tatctaatat
     4081 aaattttata ttatttgctc tacatatatt tcctatcttt tcataataat ctatcggcat
     4141 attctcacaa taactaccag aagctactag tattttagta ttttttagta cttcctctat
     4201 atcattttca aatctctcta aatcgctttt ttctactatt ggtccttttt ctaaaaattc
     4261 tgtactaact ttattgtcct caattatgtt taagcaattt ctagtttcct gctctatttt
     4321 tgtaaattta ttttgtatat ctagttttct aagttcattt tttataaatt ctccattaaa
     4381 tccacctaga aatcccatag ctaaaggatt ttcttttaaa attttacata ccttagctac
     4441 gtttaatcct ttacctccag cagttgcatt cttacttact accctttgca cctcacctat
     4501 gtttatatta ttcactttat acattctatc tatagatggg ttgaatgtta ttactgtaat
     4561 catactagat tctcctcatt attaattttt acttttctaa acaataaatt aatctgtaaa
     4621 ataaaaaata cctcatctat aatataccca aagtggttat taccaatttg aatataaatt
     4681 attttacttg ttttgtcaat ataccataat tttataataa attaatagcc ttacttattg
     4741 aattttgatt aaactttatt cattgacata aataattcta tattctataa tcaaattgtg
     4801 gtaataacca cttagaaagg actatgtttt tatgataaaa agaaatgata aaagaccaat
     4861 atatgaccag cttgttgaga tattaagaac taagattgaa aatgagatgg aaccaaacga
     4921 tagaatgctt tctgaaagaa aaatttgtga tgaatatgga gtaagtagaa ccacagttag
     4981 actagctatg gctgagttgg aacatatggg atatatatac aaaagacatg gaaaaggaac
     5041 ttttgttgct gcactaagtc aaaattctca aaacttaatg gaaagttata gctttacaga
     5101 ttatatgaga atacaaggaa aaaagccagg tacaaaggta ttatcatttg aggtagtaga
     5161 aagtacaaag tatttctctg aaaaattagg tataactcct ggtgaaaaaa tgataaaaat
     5221 aatgcgttta agacttgctg atgaccttcc tatgatgctt gaaagaacat atcttcctat
     5281 gaaagaattt ataggattga caaaagaaaa aatcgaacaa aaacctttat atgaaatatt
     5341 tagagaagat tatggcgaga ttattaaagt tgctgatgaa gaattttcag ctggaataat
     5401 ctctgataag gaagctaagt tattggaagt accaggagat tcaccttgtc tgaaattact
     5461 tcgtaccaca tataatgatg acaatatagt tattgaattt actttgagtg ttgctagaag
     5521 cgataaattt gtatatagag taagacatgt aagatagtga attaaatagc tttcaagaaa
     5581 atagaatgat attataaacg tcaatgccac aatgatagta taattataag gaggttattt
     5641 tgaaaagttt tatttatgca gataaatttt ttatgaaaca tggaatcaaa gaaaaaggtt
     5701 accttagtat aattaatggc aaattcggtg aatttcaaca agaaagacct aatattactg
     5761 ataatgtcat agatttttca ggtaaatata ttgctcctgg cttagttgat actcacattc
     5821 acggtctttt aggtgcagat gtcatggaca atacatttga agcaataaac acaatttcaa
     5881 aaggcttatt aaaatatgga gtaacatctt tcctacctac aactttaact gattctgtag
     5941 atacactaag tgattctgta gaaaatataa gcaatagctt caatcgagtt gagggtgcaa
     6001 aaatacaagg gatttattta gaaggtccat tttttacaga aaaatataag ggcgctcaaa
     6061 atgaaaaata ttttaaagac cctgacatca atatattaaa attatggcaa agaaaatcga
     6121 aaaatttaat aagaaaaata gcaattgctc cagaacgtaa aggttcaata gaattaacaa
     6181 gatatgcaac taaaaataat atctctgttt ctcttggcca tagttcagct acatttgaac
     6241 aagttaaaca agtcgttaat tcaggtgcta aagtgtttgt acatacatat aatgggatga
     6301 gccctttaaa tcatagagaa cctggaatgg ttggggctgc tatggtacta aaagatactt
     6361 atgctgaatt aatttgtgat ggtcatcatg taagcccaat agctgttaaa ataatgatgg
     6421 atgcgaagtc tagtaataac atagctttaa ttacagattg tatgagggct ggtgctatgg
     6481 aggatggaaa atatactctt ggtaagttta gtgtaaatgt aaaaaatagt gttgcaagat
     6541 taaatagtgg gagtttagct ggaagtgtat taactatgga caaggctgtg agaaatatag
     6601 ttgattggaa catagccact ttagaagatg caataaaaat ggctacatat gttcctgctt
     6661 taagttgtaa tattgataat gtctgtggct ccatacatca aggtatggat gcagacttta
     6721 tagtaacaga taagaacttt aatatatacg aaacttatat aagtggtata tgtatgtaca
     6781 aagttaataa atagattcaa tataaagtta tggaggcttc ctaaaaatag agataagtct
     6841 ctttttcatt gttaaaatga tgaaaataca tttgatttca tacaaaaaga gtgcctcata
     6901 aactaaaaag ttaattttga gacactcctt ttattaaggt tctttatttt acaatataag
     6961 tttataaaat taagaatctg gaaatattta tgtgtattct ttgaataaaa atttattttt
     7021 tagtgataat taaacattta ttggtaaggc tatggcttta aaaatcatag ccttattttg
     7081 atattttttc catttttgta aaaattatta cccctttaag aaaattcctt aatttttttg
     7141 aaatatttct ttattaattt agagttaaaa ttctttcttc aaaataaata cttaaatgag
     7201 ctaagatttg agtccaattt ctaatctttt gagtccatct ttcagaaata tccatagttg
     7261 ctaagtataa tgatttgtgc aaagaatcat ctaaaggaaa aatagacttc gatttagtaa
     7321 cttttcttaa taatctattg taactttcca ttgaatttgt agtatatatc aatgttctta
     7381 tctcttgagg atatttaaag tatgttgcta gctcatttca attgcttcgc caacttctta
     7441 atgctatcgc atattgatta ccccattttt cctctaagag cgatagctca cttaatgcta
     7501 cttcctctgt aattgcagca tatactttct tttaatcttt tgcacattct tttctatttt
     7561 tatatgatac ataatttaaa gtatttctaa tttgatgtac aacacatctt tgtatttcag
     7621 tatcaggaaa tactgcttta attgcatcag aaaatccaac taatccatct acagaagtta
     7681 ttaatatatc cttaactcct ctgtttttga catctgtaag cacctttagc caatactttg
     7741 aactttcatt ttctccaatc catattccta agacctcctt tataccttct aagttaacac
     7801 ctatggctat atatgcagct ttgtttatta ttctaccttc acttctaact ttataatgta
     7861 tagcatccat aaatacaatt ggataaacca tatcaagagg tctgttttgc cattcatgag
     7921 ctaaaggtag aactctatca gtaattttag ctactagaga atgagatata tcaattccgt
     7981 aaatactctt aaggtggctt gatatatctc gagtagacat gccttttgag tacattccaa
     8041 tcacttggtt ctctattcct gatatatcat ttttataatt actaattacc tttggttcaa
     8101 aatctccatt tcggtctctt ggaatattta aagttatttc tccaaaatct gatttaacat
     8161 ttttatttct atatccattt ctagagtttt tagtattttt attcttattg ccatatctat
     8221 cataacctaa atggtcgtca agctccgctt ctaacatttg atgaattgta ctcgcaaata
     8281 aatcttttag catatcttga atatctgtag ttgttttaac atcatattct tcaataagtt
     8341 tttttaatat ttcgtttttg ttaaacataa aaagtgtgca ccttccttta tatagtgata
     8401 tttaatattt taattatcac cactttttag aagaatacac actttatttt aaagacccat
     8461 ttattgtaaa gataaagcat ggtctaaaat ttcaaatttt agaccataat cttatgtttt
     8521 tagataggcc taaaatattt agattagaaa ataatcaatt ttaagacatc cctattttat
     8581 ttataattat gtacatttta cactatcagt taattagaat tatttttatt catatttaaa
     8641 ctattcaaat tgttttccca attttttatc tccttcttaa tgtcttcact taaagtatca
     8701 cttgaagaca ctttctttaa tttatcattc aaagatttct tttgttcttc tgaaagatct
     8761 gtttttattg taggatcaat aatatctatt acatctttta tatcaaaatc aacaaatttg
     8821 cataattcat ctaaatgatc aattaagaat tctatatatt catatgacca ttctttataa
     8881 ttttttttgt tttttaacat gaactcatta atttcataac tttgatttgc taattcaaag
     8941 aatgaacctg aaggatccat tatgtcattt atagagtttt catagttaac aatattaatt
     9001 tgtcttatta atttctcttt ttctgttaag ttactataat ttttgttaac aaaagcaact
     9061 aatccaaatt caaaatttaa aattgaatct ttacgctgtt ttactgaatc ttctccaaat
     9121 tctctaagac cttctaaatt tttatcaata gccttatctc taatttttgt taattgttct
     9181 atacttaaag cttctaagtt tttaggcact atttcttcat tatttttagc aatttctttt
     9241 gatttatcat ttgtatttct attattgaaa acaaatatag cagtgaataa acttaaaact
     9301 attactaaaa caattaaact tacatttttt ttgtttaaca tatcaaatct cctttttaat
     9361 aagtatttta tttgtgaaaa aataactcag tttattagaa taacctttat tactgattat
     9421 tctaaagaat acaatagaac acttttttga tatagtttat ataattataa cgaatgataa
     9481 actaattttt agacaaattt atataattct tttattaaat ttttaaatca taaaaaagag
     9541 actaaattcc actagtctct tttttatgat tatgtaataa aatttaatag ctgcttcaac
     9601 taaatactta ttggatcttg aaatagatat ttattctttt caattttcat ttaattaaaa
     9661 gtataaattt ttatttattc ccaataaact ttgtaataag aaattttctt tccttcaagt
     9721 ttttttaaca aatttgttct tgcaccttca tctgaatgtg cagcatatga aaaattatat
     9781 ccatttgaat gcttatctgt agatattagt gtatgagttg ctactccact actatttaat
     9841 atacttacag gtgttgctgg tcttaaaaaa cttaaagagt ttgatacagt aagtgtttga
     9901 tatgcaattc gattcatcca atgtatttta aattctcttg ctccagccca agaaggtgat
     9961 ctattactag tagaataata ataccatgga ttattattcg tccagaatgt tactccagct
    10021 tcatgtaagc attgtgaaac aaagttagca caatctccat atccatcttt ttgaaaatta
    10081 taatatggag aaggtgcatt attttttcta tttgtactac cataataacc attagtatag
    10141 ccatgttttt tagcataagc taatgcttgt tgtctataac tagaagggct tactcttggt
    10201 aaaggttcat ttgaattttt ctcattaata tttaaattat tttcccagtt ttctatttca
    10261 tcttttatat ttgtacttag agtattactt gaatacattt ttattaattc attatttaga
    10321 gatttctttt gattatctga aagatctggt tttattgaag gatcaaccat ttctattaca
    10381 tattttgcat caaaatctac aaaattacat aaatcctcta aatgctcaat caaaaactct
    10441 acatattcat acgaccactc tttatagttt tttccatttt ttaatacaaa ttcattaatc
    10501 tgataacttt ggtttgttac tttgaaaaaa tcgcctgaag gatccattat atcatttata
    10561 gaattttcat actcaacaat attgatttgt cttattaatt tctctttttc tgttaagtta
    10621 ctataatttt tgttaacaaa agctactaat ccaaattcaa agtttaaaat tgaatcttta
    10681 cgttctttca ctgaatcttc tccaaatttt ctaagacctt ctaaattttt atcaatagct
    10741 ttatctctaa tatctattaa ttgttctata cttaaatctt ctaagttttt agagaccatt
    10801 tcttcattat atttggttac atcttgtcct ttcacattag tattttcatc agcgaaaaca
    10861 aatacagtag ttgataagct taaaatcatt gtcataataa ttgaacttat atttttttta
    10921 tttaacatat taaacctccc tctttaatat atgtattaat acttgccaaa acaaaaatca
    10981 tattctacta aaatttaaaa caagtttatc accgtttttt tctaaaagag cgtttactaa
    11041 atatcataat
//
LOCUS       DAELXX010000120        11043 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.121, whole genome shotgun sequence.
ACCESSION   DAELXX010000120 DAELXX010000000
VERSION     DAELXX010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11043
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.121"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            447..1340
                     /locus_tag="KPH29_003099"
     CDS             447..1340
                     /locus_tag="KPH29_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HBG1468671.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     ILKLEEGDSHHHSNCGCGGNK"
     gene            1340..2734
                     /gene="gltA"
                     /locus_tag="KPH29_003100"
     CDS             1340..2734
                     /gene="gltA"
                     /locus_tag="KPH29_003100"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HBG1468672.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLNINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKTKVNA"
     gene            3100..5319
                     /locus_tag="KPH29_003101"
     CDS             3100..5319
                     /locus_tag="KPH29_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG1468673.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLRPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIVKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNIKWTNWTLVSKCNIFKNFK
                     NLIVNFMIKLYFYIVIFMILWKLSNAKLIEQLKKLAYYDSLSGIKNKEKFRKDSMYIL
                     KNYYQDNFYLVQLDVNKFKYINEMFGYAEGNKILIHISQVLNNNTNKYEICARMDNDH
                     FILLIACNTEDELLNRLSKINKEICNLNTTNSSKYKIVMSSGIYKITKKDDIKKIDLL
                     IDRANIAAKSKKEKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYS
                     LDDVNEIEGAEALIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEVCKTLNKWI
                     NKGYTPVPISVNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILI
                     DIMKKIKEIGFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISK
                     IVEMAKAIDIKVICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
     gene            5466..6212
                     /locus_tag="KPH29_003102"
     CDS             5466..6212
                     /locus_tag="KPH29_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1468674.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGASIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PTILLADEPTGNLDSKSTDDVLKLFTRINKEQNVTTLMVTHEVYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            6212..8092
                     /locus_tag="KPH29_003103"
     CDS             6212..8092
                     /locus_tag="KPH29_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1468675.1"
                     /translation="MDFRKFAYNNIVRNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELTILGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEIYFPVKAILLTIVS
                     FMILFLITGPMSLKLLRKKSILELLVGTKKPKKEIKSSRLFGIIGVICLILGYAMILF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGEASKNNFPMVYSYFSEHNNEKEYEE
                     LQQIREAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKELGLDKISSKLEKYEGIII
                     PSSNSQEHINSLKQLKNYSIKNLNIEVKKSKNKIIFPTGLFSKVVVISDDLYDEVKGE
                     FSKINFHGFDYKNWQSSAKITENLKEKHFNLDREYNRSYFLTLPDMYLVELQQSKILQ
                     FMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNKIVSIEIGTLF
                     FIPYFIAIINSIISLLFLNQVLNNSFRLENLGILLLISSIYSLYFYLLKKIYTRKVWF
                     FD"
     gene            8211..9602
                     /locus_tag="KPH29_003104"
     CDS             8211..9602
                     /locus_tag="KPH29_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG1468676.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            9709..10749
                     /locus_tag="KPH29_003105"
     CDS             9709..10749
                     /locus_tag="KPH29_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG1468677.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISIDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
BASE COUNT         4373 a         1072 c         1935 g         3663 t
ORIGIN      
        1 agagtatatt ttatataata tgaaagctgg tattgcaata gattaaagcc ttatacacgt
       61 ttagttttta aagaaattag tgttattatg aaaacctaag ttaatttatt aaataaataa
      121 ttgaataaaa aaattttatt atgtatttat aaaatttttg ttacaaattt ggcataatat
      181 agtatacttt aagtgtgtat taggttaaat ctataacaaa ttagacgtat gatatagact
      241 tagtgtttat tggcatttac aagaaatttg ttaatatcaa ctatttgtag tttgggcagt
      301 tataattact atagggggga tgtccaatac aaaacaatca accaagaact aatattatat
      361 tataaagagt tatagttttg tgtgtttatg tttttaatta aatttaaagt gggaaaaata
      421 tatttgttag aatggagaat agcgagatga gtaataaaat agttagtaag agacaattaa
      481 cagatagtat ttatttaatg gaaatagagg cacctagagt tgcaaaatct tctcaaccag
      541 gtcaatttat aataattaaa aatgatgaaa agggagagag gattcctctt acaatagctg
      601 actatgatag agaaaaagga acagtaacta ttgtattcca aacagttggt gcttcaacta
      661 aaaagttagc aatgtttgaa gaaaacgatt ttgtaatgga ttttgtaggg ccattaggac
      721 aagcaagtga atttatacat gaagatatag aagaattaag aaataaaaag atattatttg
      781 tagctggtgg agttggttct gctccagttt atccacaagt taaatggttt aaagagcatg
      841 gattagatgt agatgtaata attggtgcaa gaactaaaga attaattata ttagaagatg
      901 atatgaaaaa agtagctaaa aatgtatatg tatcaacaga tgatggtaca tatggattta
      961 atggaagagt tacagaccta ttaaaagatt tagttgataa tcaaggtaaa aaatatgacc
     1021 aagcaatagt aattggacca atgataatga tgaaatttat gtgccaatta actaaagaac
     1081 taaatatacc tactatagta agtttaaata caataatgat agatggaaca ggtatgtgtg
     1141 gtggttgtag agttagtgtt ggaaatgaaa ctaagtttgc atgtgtagat ggtcctgaat
     1201 ttgatggtca tttagttgat tttgatcaag caatgagaag acagtctatg tacaagactc
     1261 aagagggaag agctattcta aaacttgaag aaggcgatag tcatcatcat agtaattgtg
     1321 gatgtggagg taacaaataa tggatgctaa aaaagtaaaa gtaccagtta gagaacaaga
     1381 acctgctgtg agagcaacaa attttgatga agtatgctta gggtataata aagaagaagc
     1441 tatggcagaa gctaatagat gtttagcttg taaaaaacca aaatgtgtgg gtggatgtcc
     1501 agtaggaatc gacataccag ggtttataac aaaaattaaa gaagatgata tagaaggtgc
     1561 tgctaaagta atagctaaga gcagttcatt accagctgtt tgtggtagag tatgcccaca
     1621 agaaagtcaa tgtgaaggtg tatgtatact tggaataaaa tcagatgctg tatcaatagg
     1681 taaattagaa agatttgtag cagattggtc aaaagaaaat gacataaatt tatctgatac
     1741 agagcctaag aaaaatcaaa aagtagcagt aataggaagt ggtccagcag gtctagcttg
     1801 tgctggtgat ttagctaaaa aaggctatga tgtaacaatc tttgaagcaa tgcatgagcc
     1861 aggtggagtt ttaacttacg gaatacctga gtttagactt ccaaaacaag ctgttgttca
     1921 acctgaaata gacaatataa gaaaattagg tgtaaaaata gaaactaatg taattgttgg
     1981 taaaacaatt acagtagatg aacttataga agatgaagga tttgaagcta tattcatagg
     2041 ttcaggagct ggtcttccaa tgtttatgaa tataccaggg gaaaatgcaa atggagtgtt
     2101 ttctgcaaat gagtttttaa ctagagtaaa cttaatgaag gcttatagag atgactatga
     2161 tacacctatt agttctggta aaaaagttgc tgtagtaggt ggtggaaatg ttgctatgga
     2221 cgctgctaga actgcattaa gattaggttc agaatcatat atagtttata gaagaagtga
     2281 aaaagagctt ccagcaagag ctgaagaagt tcatcatgca aaagaagaag gcattatatt
     2341 taatacatta acaaatccta aggaaatttt agtagatgaa aatggatatg ttaaaggtat
     2401 ggtttgtata agaatggaat taggagagcc agatgattct ggaagaagaa gacctataga
     2461 aatagaaggt tctgaatttg tacttgatgt agacacagtt ataatgtcac ttggaacaag
     2521 cccaaatcca ctaatatctt ctacaacaaa gagtcttaat ataaataaga agagatgctt
     2581 aataacagat gaaaatggtc agacttctaa agagggtgta tttgcaggtg gagatgctgt
     2641 tactggagca gcaacagtaa tatctgcaat gggtgctggt aaaacagcag cagcatcaat
     2701 agatgaatat ttaaaaacaa aagttaatgc ttaatttgta aattaaagag agggtgtctc
     2761 aaaatgaact ttttagttca tgaggcactt tttttgtaca aaatcaaata tgttttaatc
     2821 atttcaacaa tgaaaatttc attcctgtag aaagtctaac ttttaagtct tagtacaatc
     2881 tctattttga tgtaggcact tttttagatg gtttaataat ttatcagtta ttattgtaat
     2941 atttgatact aagtaacata actttgtcta actatgataa aatagttata caataaaggt
     3001 cttgtaagga gtgaataatg tgtggctaat aaaatcagat ttgtcaaatc taacattaaa
     3061 taatagaaaa ataaattgtg taattaatgg tattttaata tgaaaaaaag agaaaaagta
     3121 tttgatgggc tagtttttaa gcatatatta atcttattaa tactattagt tttgattgat
     3181 ttaacaagta ccatcatata tgcaagtaac tctatggaaa ttacttctag aaatatgatt
     3241 gaggcatcaa aaagagaact tgaaaactat ttagaagtaa acatatcttt attaaaagct
     3301 cttagtcaag atgatagatt ttctaatggt gaaacatcac taattgaaaa gggaaaatta
     3361 ttaaggccat atcaaaagga atataatctt tttatgattg gtataacaga tacaaaagga
     3421 aatgcttcaa gtacatatag ggaaaatgtt aactcaataa aagataagcc tagttttgag
     3481 aaagcaatta aaacaaagca agtagtagtt tcagatattg aagtcagtaa tgtaacaggt
     3541 gacaaagtat ttatcatata tgtaccaatt gtaaaaaata atgagatgat tgggaccatt
     3601 tttgcttctt tttattttca agatgtaaat aatttaataa gtagaagcaa ttttgatgat
     3661 tctattaaat ttctaatgat agataaagat tatactatta tttctcatcc aaacaaaaaa
     3721 tatgtaaatg ataaaagtaa gatgcttgat ttagaaggga atattatagg aaccactaaa
     3781 agtgaaatct taaaaaatat aaataaaaaa tgtcaaggtg attttttatc ttgggataat
     3841 tggagattat ataatgtaaa atatacgaac ataaagtgga ctaattggac tttagtatca
     3901 aaatgtaata tatttaagaa ctttaaaaat ctaatagtta attttatgat aaagttgtat
     3961 ttttatatag taatatttat gatattgtgg aaattaagta atgctaaatt aatagaacaa
     4021 cttaaaaaat tggcttatta tgattcttta agtggaataa aaaataaaga aaagtttaga
     4081 aaagattcta tgtatatttt aaagaattat tatcaagata atttttactt agtacaattg
     4141 gatgtcaata aatttaaata tataaatgag atgtttggat atgctgaagg gaataagata
     4201 ttaattcata tatcacaggt tttaaataat aatacaaata aatatgaaat ttgtgctagg
     4261 atggataatg accattttat attgttgatt gcttgtaata ctgaagatga acttttaaat
     4321 agattatcta aaataaataa agaaatttgt aacttaaata ctactaactc atctaagtat
     4381 aaaattgtta tgtcaagcgg aatatataag attactaaaa aggatgatat taaaaagatt
     4441 gacctgttaa ttgatagagc aaatattgca gcaaaaagta aaaaggaaaa atatgaacat
     4501 tcatattcgt tttttaatga agatacaaga aatcgattgt acaaggaaaa acgattggaa
     4561 gataatatga ataaagcttt agaaaaaggt gaatttatag tatattatca acctaagtat
     4621 agtttagatg atgtaaatga aattgaagga gcagaagcct tgataagatg gaatagtcca
     4681 gaatttgggt ttatttcacc tatagatttt gtacctttat ttgaaaagaa tggttttatt
     4741 gtaaatattg atatgttcgt gtttgaagaa gtatgcaaaa cattaaacaa gtggataaat
     4801 aaagggtata ctcctgtacc aatctctgta aatatgtcta gagttcattt atatagggat
     4861 aattttatag aaaatataac tgatttgata agtaaataca atatatctcc tgagtttatt
     4921 gaacttgaac taacagaaag tgttgtgttt gataatttaa atatattgat tgatataatg
     4981 aaaaagatta aagagatagg attccttata tcaatggatg attttggttc gggatattca
     5041 tctttaaatc tgttaaaaga cctttctttt gatattttaa aattagatag aggattttta
     5101 atcgaaacaa cagatacaaa acgtggaaaa attattattt ctaagattgt ggaaatggca
     5161 aaagcgattg atattaaagt aatctgtgag ggcgtagaaa cttatgaaca agtagagttc
     5221 ttgaaagaga taggttgtga taaggttcaa ggatacttat ttgctaaacc tatggtatta
     5281 gatgaatttg aaaaacactt aaactttaaa tttgattaat cattaataat tattagacta
     5341 taatataccc taacaactta cttacaaata tgtaagttaa ttgttagggt atttttatgg
     5401 ttaaagtagt atattcacga tattatggta ttgtagtaaa aataaaaaat tatggagaat
     5461 ataaaatgaa catattgagt attagaaata tatcaaaaac atattgcgga aatattccat
     5521 ttaaagcatt agataaggtt agtctaaata tagaaaaggg agaatttgtg tctgttatgg
     5581 gaccatctgg aagtggtaaa tctactcttt taaatataat atctacagtt gatagacaga
     5641 gtgaaggtga ggttgtatta gatggatatg atgtaagtaa attgaaagga gaaaaacttg
     5701 ctgaatttag aaggaaacaa cttggttttg tatttcagga ttttaatttg attgacactt
     5761 taactgttgg cgaaaatatt atgttgccac ttacactaga gggggcaagt atcaaagata
     5821 tgaatataca aactaaaagt atatctaaat ttttaggaat agataaaatt ttagatagaa
     5881 aaacctatga aatttcagga ggtcaagctc aaaggtgtgc tattgctaga gctattataa
     5941 ataaacctac catacttctt gcagatgagc caactggaaa tcttgattca aaatctactg
     6001 atgatgtttt gaaattattt actaggataa acaaagaaca aaatgtaaca acacttatgg
     6061 taactcatga agtatatagt gcaagttatt cagatagagt aatttttata aaagatggtt
     6121 gtatatatac agaaataaaa aaatctgaaa gcagtaattc tttctattct gatatactag
     6181 ctgttttgtc tcaaatagga ggtgtaagat agtggatttt agaaagtttg cttataataa
     6241 tatagttaga aatttcaggg catatttagc atatttttta agttctgttt tttctataat
     6301 gatatttttt gtttttgcag tgtctatgtt ccatccgatt ataacaaaat ctggaatgca
     6361 gagtggttct actgcattta tggctcttat gacttcagaa ttaacaatat taggttttag
     6421 tctacttttt atattgtact ctttaggaaa tttcttgaaa tatagattga aagattttgg
     6481 agtattgatg ataattggaa tgagcaacag acaactgaaa aaattgatat taattgaaaa
     6541 tttgataata ggatttttag cagtgatgtt aggaatatta cttggtttat caatttcaaa
     6601 attgttttta ttgtatttaa gcaaactgtt ttatatgaat ttaactgaaa tttattttcc
     6661 agtaaaagca atactattga ctattgtatc atttatgatt ttatttttga taacaggacc
     6721 aatgagctta aaattattga gaaaaaagag tattttagag cttttagtag gtacaaaaaa
     6781 gcctaaaaaa gagataaaat cttcaaggct atttggaata attggtgtaa tatgtcttat
     6841 attaggatat gctatgatac tatttggcaa taagttaaaa ataaatggag gtatgtatac
     6901 aacagcacta ttaattactt tggggatatt tttatttttt tctcaattta ctattttgat
     6961 attaaagtat ttaaaaaata aagaaaaatt ttataagaac aaagtaaata tgttacttat
     7021 aagtaatttg atatataaat taaaggataa tacacgattg ttatttttaa taacaatact
     7081 tttatcggct actttagtag catttacaac aacttcaaca cttgtaagtt ctcaaggaga
     7141 agcttctaaa aataatttcc caatggttta ctcatatttt tcagagcata ataatgaaaa
     7201 agaatatgaa gaactacaac aaattagaga agcaatcaaa ggatatgatt ataaggagtt
     7261 aagttttcct gtacttaaat ataataggga agtcttacta agtgtaaaga attataacaa
     7321 attatcaaag gaattgggat tagataaaat tagttcaaag ttagaaaaat atgaagggat
     7381 tataattcct agtagcaatt cacaagaaca tataaattct ttaaaacaat taaaaaatta
     7441 tagtataaaa aatttaaata tagaagtaaa aaaatcaaaa aataaaataa tttttcctac
     7501 aggactgttt agtaaagttg tagttataag tgatgactta tacgatgaag taaaaggtga
     7561 attttcaaaa attaattttc atggatttga ttataaaaac tggcaaagca gtgcaaagat
     7621 aactgaaaat ttaaaagaaa aacattttaa tttggataga gaatataaca ggtcatattt
     7681 tttaacatta ccagatatgt atttagtaga gttacaacaa agtaaaattc ttcaatttat
     7741 gggtgttttt ataggagtga tattatttat agcagttttt agttttttat acttcagatt
     7801 atatacagac gagatagaag ataatgtaaa gtacaaaaaa ctatcaaaaa taggtctatc
     7861 ttttaaaaat atgaataaga ttgttagtat agagataggt acattgtttt ttataccgta
     7921 ttttatagca attataaata gcataatttc tttactgttt ttaaatcaag tattaaataa
     7981 tagttttagg ttagaaaatt tgggtatatt actactgata tcatctatat attcacttta
     8041 tttttactta ttaaaaaaaa tttatactag aaaagtttgg ttttttgatt aaagtggtat
     8101 ataatagata tataaattaa tatttaaaaa gtagttaata ttaggggagg gcatttgcac
     8161 ctcccttgta atatgtggaa aattgaatat tagaagaagg aggagacttg atggaaaatc
     8221 aacaattatt aggaacagaa cgaattagta aattactact aaaatattct ataccagcta
     8281 taataggtat gttggtaaat agtttatata atgtagtaga tagaattttt attggaaata
     8341 taccaggggt tggaccactt gcaataacag gtcttggtgt aacaatgcca ataatgacaa
     8401 taatacttgc atttgggatg ttgataggta taggaactac tactaccata tcaataaaac
     8461 ttgggcaagg taaagtagaa gaggcaagaa aacttatagg aaatgcaatg acgttatctg
     8521 taataacagg aattatcata atgatacttg gaatattatt tgcaaataaa atacttacat
     8581 tgtttggagc aagtgaaaat actttaatat atgcaaaatc atatataaat attatattat
     8641 taggtacagt agtaaatctt ctttcctttt ctttaaacca ctcaataaga gctgatggta
     8701 gtcctaaaat ttcagcagga ataatgatag taggatgttt aactaatata gttttagact
     8761 ggatattaat atttggattc aatttgggaa tacaaggagc tgcaattgct acagtaactt
     8821 ctcaggctct tacagctatt ttaactatag ggtattatat aagtggaaaa tcaaacttac
     8881 gattcagtaa atctaatcta aaattagata aaaagctaat aaaagcagtg tttgcaatag
     8941 gtatgtcgcc atttgcgatg caacttgcag caagtttagt tcaagtaata tcaaatattg
     9001 cacttaaaac tcatggtgga gatttagcta taggggctat ggctactatt tcttccattg
     9061 caatggtatt tttgatgcct atctttggaa taaatcaagg agcgcaacct attataggtt
     9121 tcaattatgg agctgaaaaa tatgataggg ttaaaaaagc atatttaggt tcattagtag
     9181 tagctactat aattttatgt atgggtatgg tagtagttat gcttttccca gaagctatta
     9241 taggtatatt taataaagac cctgaactta tgaatatatc tgttaatgga ttaagaatat
     9301 acttacttat gttgccaatt gtaggcttat cagttacagg aacaaacttt atacaatcta
     9361 taggaaaagc aaaaatggct atgttattga gccttttaag acaagtaatt ttattgatac
     9421 cagcagtatt aattcttcct acattcttag gtttacaggg tgtatggaca gctcaaccag
     9481 tgtctgattt tatagctaca gtaattacag gtatagttgt atttagagaa ttaaaaagat
     9541 atactcctaa aactgaaaaa ttaaatgaaa atgaaagact aaatgagata actactgagt
     9601 aaaaatataa attcagataa ttgagcaaga aattgcaaaa aatacgatta tatgataaaa
     9661 taaaaccgct gtcaaggata agagaaattc taattatgga gtgattttat ggaatttaca
     9721 aagaaaagca aattaaaaaa tatgtatgta aacatgaatg tagctagaat gttgtctaag
     9781 ataaatgagt ataagggtag gcaactgctt tacaaaaaac aaccaaaaga aattttagaa
     9841 aacctagaaa aaaaatcttt agtagattgt tctgaatcta catacattgg aaatcaaaga
     9901 gatagtagta attttaatct agaaaagttg ataagtaatg aagttacacc tagaagtaga
     9961 gaggaattga gtattgttga atacagagat gtggtaaaaa ctattaacag tgcttatgag
    10021 agtattccca tcagttcaca aacgattctt gagcttcatg ggtacttata taaattttca
    10081 tctactagag gaggaagtta taagtcagat aatgatttta ttgaacacaa tttaaaacct
    10141 agcgaattta taagtattga ttcaagtgtc aataaagttg aaaaagctgt agaagaaata
    10201 tgtgaagctt ataatacatt gatagaggaa gatgaaattg atatattaat attaatttct
    10261 gcatttgtgt tagatttcat attgatacat ccattcaaag aaggtaatat aaaaatggcg
    10321 agagtactta tcctattgtt actaaataaa aatgggtatg aagttggaag atatataagt
    10381 ttaggaaaga tatttgatga tagttcatat gaatattata gtaatttaaa ttatttgaaa
    10441 gcctcaatag gtagtgaaaa agcagatatg aatgcatgga ttgagtattt tttagagacg
    10501 atattaactg catatgagaa gctggatgat agtttaaata tatctgataa gaaaagacaa
    10561 acaaaaacta gtagaataga aaaaataata aattctaccc ttggatattt tacaaaagaa
    10621 gatataagag atttatgtcc tgacattcca gaacctacta taaatagagt ttttaataat
    10681 ctaagaaaac aagataagat agaagttgtt gcaagaggaa gaagtgcaaa atggaagaaa
    10741 aaatattaaa aaatgtttaa ataactaaaa tggtatagtg ttttacacta taccattttt
    10801 aatgtcatta gcatgagcaa taatttaaca acttaagcaa tatcaatttt atttattgat
    10861 tatttttgaa ctgaaaaatg gggttatatg gtatactaca ataataatta attttggatg
    10921 agaatatata acgttacaga atatcttagg agtatatgta taaaaaatat aataaaagtg
    10981 tgaggtttag taattaacaa attatgtggc ttcacacttt tttataaaat aaaaatactt
    11041 agc
//
LOCUS       DAELXX010000121        10896 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.122, whole genome shotgun sequence.
ACCESSION   DAELXX010000121 DAELXX010000000
VERSION     DAELXX010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10896
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.122"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(34..699)
                     /locus_tag="KPH29_003106"
     CDS             complement(34..699)
                     /locus_tag="KPH29_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBG1468678.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            complement(850..1011)
                     /locus_tag="KPH29_003107"
     CDS             complement(850..1011)
                     /locus_tag="KPH29_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HBG1468679.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            complement(1089..1598)
                     /locus_tag="KPH29_003108"
     CDS             complement(1089..1598)
                     /locus_tag="KPH29_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBG1468680.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            1804..2022
                     /locus_tag="KPH29_003109"
     CDS             1804..2022
                     /locus_tag="KPH29_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG1468681.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            2040..3986
                     /gene="feoB"
                     /locus_tag="KPH29_003110"
     CDS             2040..3986
                     /gene="feoB"
                     /locus_tag="KPH29_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBG1468682.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIMEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKSNTH"
     gene            complement(4146..5201)
                     /locus_tag="KPH29_003111"
     CDS             complement(4146..5201)
                     /locus_tag="KPH29_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG1468683.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRANLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGTISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(5249..6049)
                     /locus_tag="KPH29_003112"
     CDS             complement(5249..6049)
                     /locus_tag="KPH29_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG1468684.1"
                     /translation="MNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     KVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFLIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            complement(6042..6833)
                     /locus_tag="KPH29_003113"
     CDS             complement(6042..6833)
                     /locus_tag="KPH29_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG1468685.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMFDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTTLINAIPKVILDGLGVAGTLLPAVGFALLMDMLISKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAMNTLTEGEIDFE"
     gene            complement(6873..7295)
                     /locus_tag="KPH29_003114"
     CDS             complement(6873..7295)
                     /locus_tag="KPH29_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBG1468686.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLANIEYFCMTKDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSIFL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENRNKIKNVM"
     gene            complement(7311..7790)
                     /locus_tag="KPH29_003115"
     CDS             complement(7311..7790)
                     /locus_tag="KPH29_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG1468687.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTRDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKEIGDLGIDIELRMLPRDKKKQLSDLIK"
     gene            complement(7922..10678)
                     /locus_tag="KPH29_003116"
     CDS             complement(7922..10678)
                     /locus_tag="KPH29_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG1468688.1"
                     /translation="MRDEILSFVNECVLFNNFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYDNSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSKFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNTILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEIFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALESKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDTIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKNKINGPMLFIDQISTISALEVGNLLMQGKNIEQIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGTAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLPNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
BASE COUNT         3590 a         1874 c         1265 g         4167 t
ORIGIN      
        1 tttattcact ttacaaaata tattgtatat attttagtcc aaataatcta catttattac
       61 tccttctatc atatttattt ctttcataac gtcgtgtaag tgtattctct tagcataaga
      121 tacaaaaaaa ttaaccttcc agtattcttc attacgacat tctatttcca tatttcttac
      181 gataagttga tttttttcaa atttatcata taaaaaaggt atagagtcca aattgcttaa
      241 ttttaatatc aatacattct tttcttggct aaataaaaac gtattgatta tcctaagcac
      301 aaataatgct atcattacaa ataatccacc tagaattgcc aattcataat aaccatatcc
      361 aactgctata ccaatacatg cagttgccca catcccagca gctgtagtaa gcccttttac
      421 tccaccttta ttttttaaaa ttgctcctgc acctaaaaaa ccaattccag ataatacttg
      481 agcaggcatt cttgctgggt cagcatttgt atacaaatta taatcaaaga ataattgaat
      541 cgaagttatt gaaacaatac aagaacctat tgcaattagt atatgtgtcc taaatcctgc
      601 aaactgtctt attttttctc tctctaagcc tattattcct cctattatta gtgctaatag
      661 taaccttatt gctatctcct tacttcctat atcaatcata atataaaacc ctcctgactt
      721 aattacaact tatgtcctac agataattta cacatcagaa atactatttt atatcttaat
      781 attattatta atatatgtaa aatatagcca tctgttaatt tttgatacaa aaaaacccac
      841 aactgtgggc tatttgctta ggtacatgta tttatttatt aatatatcta ttttcttact
      901 tgattctact aactcttctt gaaatatttc tggatattct atgtatgtat ttatctcttc
      961 cctaactgct tcaatctcat tttttaacgc ttctaattca ttgttcgcca taataatcac
     1021 cactattaca aatttgtata tttttatata cattttatca aacaatatta tattttgtca
     1081 atattatgtc aataaaactt actataaaaa tgctttaatt taggtattgt atacctggat
     1141 actaagccta caaacatacc agttactaat gatgccatta acataaatgg taagtaccct
     1201 atagcaagta tattttctat gattaaactt gcaactatta attgacctat attatgccct
     1261 attgcaccta taatgcttat ccctattagt gataaatctt ttattttact cgctaaatac
     1321 ataaataaaa gactcagata agctcctcct atactaaata gtagatatga taaagggcct
     1381 gcaaatattg atgacaaaat taccctgata gtaacaatta taacagtatc tttgacacct
     1441 aacaacatta atgaaattaa agttataata ttgctcaaac ccaattttgc ccctggaaat
     1501 ataactataa gaggatttgg tatatatgtc tctagtacat ataaagctaa actataccct
     1561 atcataagac ctaaaaatgt catttttcta gttttcatta agttaactcc tccaaatttt
     1621 atctatcatc ttacattata atacaaaata tataaaaaca cctaatttag actaaaatat
     1681 ttttaatcta tttagaataa atattagtta tttcaatgtg caattacctc tataaatctt
     1741 gactttttgt atcatacttg ttacaatttt aatgataatg aatttcatta ggaggtgtta
     1801 ataatgactg tttacgattt aaaaatagga gaaaaaggat ttattaatag tatagatgga
     1861 gatattaaat tatctaaaag attgcttgca cttggatgta taaatagtac tgaaatagaa
     1921 gttaaaaagg ttgccccact tggagaccca attataatta gatttagagg ctttgacctt
     1981 gcgattagaa aatctgatgc taaaaatatt tctttacaat aatagaggag gagctacccg
     2041 tgattaatgt agcattatta ggaaacccaa atgtcggtaa aactactgtt tttaatctac
     2101 ttacaggttc aaatcaatat gtaggtaact ggcctggtgt tactatagaa aaaaaagaag
     2161 gatttcttgg aaaagagata aaggttgtcg atttacctgg tatatatgca atggatactt
     2221 tctcaaatga agaaaaggtt tctaaatcat atttagaaaa tgaagatgtt gatgtaatag
     2281 taaatgtagt tgatgcttct aatttatcaa gaaaccttta cttaacaact caacttatgc
     2341 aatttaacaa gcctatagtt atattgctta atatgttaga cattgcagaa tctaaaggag
     2401 ttaatataga tgccaaaaaa ttaagtgaag aacttggagt aattgttgtt ccaattatag
     2461 caaaaaagaa aaatggtgtt gataggatag aagaagagat taagggagct acgcaaaaag
     2521 cttttatata tcagaaaaac tttggaagtg aaacagaaac atataaaaaa ttagaatcca
     2581 ttttatcgag atgtacaaaa actacatata cacaaaagaa atctatcagt gataagatag
     2641 acaatatagt cttgaatcct atattagcat atccgatatt tattggtgta ttgttcctct
     2701 tatttaaatt tacctttgac tgggtcggag gaccactaca agagggattt gctagtctta
     2761 tagaggctta tatttctgca cctgttagtg atatgttatc taactctagc ccatggttta
     2821 aatcacttat agttgatgga ataattggag gtgttggtgg tactttacca ttcttccctt
     2881 taatatttac attattcttt ggtatatcgt tgtttgaaga tagtggatac atgtcaagaa
     2941 cagcattttt aatggataaa gttatgagaa aggttggtct atctggtaaa gcttttatac
     3001 ctatagttat gggtatggga tgttcttctc cagctataat ggcaactaga acattggaaa
     3061 gcgaaaaaga tagaagagtt acagctctta ttgcaccact tatgacttgt ggagcaaagc
     3121 tacctatata tgcactattt gtagctatat tctttccaca taatgctgct ttagtaacta
     3181 cgtcactgta tttggtgggt atagttatgg caattgtagt tgcattattc ttaaataaaa
     3241 cagcctttaa aacagaggct gaaccattta tattagaatt accagaatat agaatcccta
     3301 ctatagatgc acttattaaa aatacttgga ataaatcaaa aggtttcttg ataagagttg
     3361 ttactgtcat gtttgcaatg tcagtcgtaa tttggggact ttcttcattc aatgtttttg
     3421 gatatacaga agatataaat gtaagcttct tggctactct aggtcatatt atatctccta
     3481 tattcaaacc tcttggtttt gatgattgga gaacttcagt tgctatttta agtggtcttg
     3541 gagcaaaaga aatcgtagta aactctttaa atatactata tggtaatcta actgttgtct
     3601 taccaacaat atttaatgga gttactgcat atacattctt gatatttact gctttatata
     3661 ctccatgtat agctgcactt gcaactatga aaaaagaata tggcaataaa atgatgttta
     3721 catcatttgc atatcaattt atactagcat ggataatggc ttttatagtt aaaagcatag
     3781 gtggagtact atttatgggt aattcaataa tggaagcttt gataggtgga ataattatag
     3841 tccttgcact ctttattctg tttaataaat tcaagtctac aaaaaacgga aatgtctgta
     3901 gtggatgttc tatttgctct ggatgtccaa gtgctaattc ttgctcaagt aaggaagaca
     3961 gcaataaaaa aagtaataca cactaatata taaatatttt agtttaaaat aagataaaag
     4021 tgggtgtctc aaaattaact ttttagttca tgaggcactc ttttttatat aaaatcaaat
     4081 atattttcat catttcaaca gtcaaaaaga ggcttatctc tattttgaga caacctcttt
     4141 ttttgttact tattcagttt cttgctacct aacttcatgt ggaacctagt atcttttgga
     4201 acacttgggt ctacgcctct taatgtaggt atttccccac atattagttg tataggtact
     4261 ataaaattaa atgtttgata gtaaggatta tctgtgatat caatctttaa atttttatca
     4321 ttttctgtaa catctctacc tatagcaaat acattttccg tccaaccaga aagtacatca
     4381 atcatcttag ttactctagg ttcttttcct gtatctagta taaatatagt agaatcacta
     4441 tttatagcat tatatatacc gtgtataaac tcttcaaact catacccagt tacaggtatt
     4501 ctcattgttt ccaataattt taatgctgct tctaatgtat ctccatatat atcagagtga
     4561 cctattattc ttatttcttt agaattaact aacttttctt tgtttctttc aatccattgt
     4621 ttagagagct tatacactgc ttcaaatcta ttaattgcat ctaatatctt atttatttcc
     4681 tcattgtact tttcactaga tattgtacct ttttctctag ctatttgaag acctaataac
     4741 attaagttta actttgtaca ataatatcct tttgttttag ctccagattt ttcttcacca
     4801 caatttactg ttaatatata atcagaaatc tcatctatta aagcattctt acaacctgcc
     4861 atagatgcta ttttacaacc cttatcttca gctaacttca tagcgttgta tgtagaataa
     4921 ctactcccac cttgagatac acctactact agggtatttt cattatcaaa cttgaatgta
     4981 tcttcagtta tcataaatgg atacattttc acaacatcta tatctaataa attttgtaaa
     5041 tatggttgca cttgcactcc tgagtgatag ctagtacctg aacctgttat tataatcttt
     5101 tttagatttg ctctcttaac ttcattaaat aagctatctg cattagatat aatttctctc
     5161 attctaactg gtgtttctaa catataatct tgtattgtca ttttatacac cttccttatc
     5221 tatatattca ataaattata attaatttct ataacaaacc tatagcatgt aaagcgattc
     5281 ctgttactat agtgcctata gttaattttg ttacacttac ctttctcttc aacatgtaat
     5341 atattaaaaa tgcaaaggtt aaaggtaaca tattaggtat tattttatct agtatatctt
     5401 gtaaaattac ttctgcacca tttaagttaa atttcaaagg agtagttata ctcaacatag
     5461 ttgcaaccat tcctcctaca acgaataaac cgacagttgt agccatagac attatcttct
     5521 ccataagtcc ctctctttgt attctttcaa gagaatttac ccctacttta taaccatact
     5581 taagtccaaa tattctaagg gcaaatgctg gtatattgta taataataag aataatagtg
     5641 gtccaaagat attgccttct tttgcaagtg atactcctac tcctgctgct ataatcctaa
     5701 atgttcccca gaataatgaa tcaccaattc ctgcaagagg tcccataaga gctgttttaa
     5761 cagcactaat tgaactttca tcaaattctg gattattagc attttcttct tccatagctg
     5821 ctgttatacc taatatagca ggaactacca ctgtcgttgt attaaaaatc tctaaatgcc
     5881 tttctattgc ttttgcttga ttttctttct tattatatag cttctttatt gcaggtaaca
     5941 ttgaataaca ataacctaag ttttgcattc tttcgtaatt aaatgcacct tgaagagcaa
     6001 atgaacgcca aaacatactt cttaattctt tttttgttaa tttattcaaa gtcaatctct
     6061 ccttccgtta aagtattcat tgctaattga ggattttgat tattttcttt gttattgata
     6121 taaaagttta gtacaaatgc tacacatact ccaaataaag ctatagcagt tatatctaat
     6181 ccaccataag atgctaacaa aaaccctata aagaaaaata cagacatctt ttttgaaatc
     6241 aacatatcca taagcaacgc aaaacctact gctggcaaca atgttcctgc aacacccaat
     6301 ccgtctaata taactttagg tattgcattt atcaatgttg taactttatc agcaccaatc
     6361 ataattgcta aaaaggttgg aactgatgta cttaaaaaga ataatattac tggcaaccac
     6421 atcattatgg taactttctt aaaatcagcc tgtttagcat agaaaactgc cttttgagaa
     6481 aagtacgaat ttattattct aactaatact ccaagagatt gtgctaatac tgctactggc
     6541 actgccactg ctaaagcaac ttctgcacca ttacctgata atatagcaaa agctgtaccc
     6601 aatacacctc ctgttactac gtctggagga gtagctgcac ctatccctgc aattcccatc
     6661 caaattaatt caagctgtgc accaatcata attcctgatt ttatgtctcc taaaactaat
     6721 ccaactaaca tacctgtaac tattggccta tcaaacatag attgaccaaa tatacgccca
     6781 tctagtatac caactcctgc tataatagct aacatagtag cttgtactaa catattgaac
     6841 ctccatttta acttagttta atttacatta aattacatta catttttgat tttatttcga
     6901 ttttctaata actcatttac ataaacaatt ccattttttg cttcctctaa gcctgattta
     6961 actattagtt ttgtatcctc ctcaactaat aagaatatag ataaaagcat tggtaaattt
     7021 acgcctgtca acaattcaaa tttgaaccct tgcaaaagta agtttgtaca tatatttgcc
     7081 acacttccac caaacacatc tgttaaaaca acaaattctt cactatcttt attatttgat
     7141 aatatacttc taatttcttt ctcagcatca tctgctgatt tatcttttgt catacaaaaa
     7201 tactcaatat tagctaatga accaattatt aatttacttg tgtcaacaag ttctctagac
     7261 aattctccat gtgttgcaat tagaaatttt ttcattctct ttctcctaaa ttattttatt
     7321 aaatctgata gttgtttctt tttgtctcta ggtaacattc taagttctat atcaattcct
     7381 aaatcaccta tttctttaaa gttatttata tcatcatcat caacagcaac taaatctgta
     7441 agtttctttt ttccttcttc atatcttaac cctcctacat ttatttcttt taatactcct
     7501 cctgaatttt tagctacaac taaagcatct ctggtatttt ttactaacac taatacattg
     7561 cttttctttg aagaagcaaa tttttctact atttctccag attcctctac cccccttata
     7621 tataaagtta cattgctagg tttcgctaaa ttcagtgaca tagctttcat ttggtcattt
     7681 tttgcttcat catttgcaac tagtattaca tttataccta gtgatgttgc ccatccatat
     7741 gcaacctgac catgtatcaa tctgtcatca attctcatca aagataccat tttccccaaa
     7801 tcctccatca tacattaaaa tttttattgt ttatactctc atttatctat aaaagcaaaa
     7861 agtatgccaa atactggcat actattttaa atttcattta cttattttgt aatcttgtat
     7921 tttactgtgg tagaccatag ataatattat aaatatatcc tatctctgaa attggtattt
     7981 gcacactata actcttttct agcacactaa atgcattttt tattagtttt acttcactat
     8041 gatggttttt gattaacaaa tccatatcag tatactcttc aatttcagtt tgtctaacta
     8101 atctttcaat catacaactt aaatgaacat ataaagaaat ctttttatca tttggaagtt
     8161 ttttcttttg aatattttct atagcattga ttccttcttc tattctctct attatctttt
     8221 ttgaatctaa aatagttaga ctgtctatta ctctaattaa tgaaaaattc tttacaatag
     8281 aatcatttat gttctttatt ttttcatttt ctactactct actaaatatt ctaaaaacat
     8341 catcttctcc ttttcctgat attatatcct ctaacaatat aaaatccacc ccattcacat
     8401 ttggattagc agttcctact atagccatca catcatacat ttcaaaaact ggttctttat
     8461 ctcccataga aacaagacta tcataatcat aaggtattat tttgacatca actatcccaa
     8521 ttaaactttc ggaaagtaga tgttggattt ttatagcagt acctatccct gtaaaacaag
     8581 atgatattat agcatattct ttattttttt gaggataata caattgtata ttaggtttag
     8641 tttcttcatc cataatttct gcaatctgtt caatgttttt accttgcatt aataaatttc
     8701 ctacttctag tgcagaaata gtggatattt ggtctataaa taacattggc ccatttatct
     8761 tattttttaa atcttcatag ataagattaa gagagcccat atcaaataac acaattaaac
     8821 cattctttat cctatctcct tctaggtatc tagacatttg ttttgataca tcagcaatgg
     8881 tagcatctat aggcatatca atagctgtaa atatattttt acccaacatc cgattacata
     8941 cattcgccat actactggca gtagcatatc catgtgctat aatagtcgca ttaacttgat
     9001 taaatttcat tcttataatt gttgatttaa taaatataat cattatgatt gtatctacat
     9061 tattaagata catatccaat ctattcgtta ttaatgttga tattttactt attatagagt
     9121 attctttgta actctctttt tttaaatata ttagtaattt actttctaaa gctttatact
     9181 catcagataa ttgataagat tcgtccttca aataaagata agacgaaaat gccaatacat
     9241 tattcccatc gtattttata ttgttattaa cttctaaatt tctaaatata tcctgcatta
     9301 tcgatattat aagctccata agtacgttat tattttcatc attatttgaa aagactaaat
     9361 tgtccattag tctaatcatt agattagaac tctttttact aaatatttct tttgttattt
     9421 catttttttc tagtctatca aagtagtcta aacattttat gaaactttga tagattatat
     9481 ttcttttatt tcttccattt gacatttcaa tactaaattg tttaggagaa aagataatgc
     9541 ttttgctttt accactttgg aattgaaata tatctacatt ttctatataa agatttctag
     9601 ggaggtcttg aagagtaaca aaaatatttc catttcttct ttgatttttt gcataagctc
     9661 gtccacatgc atattttatt acatttttac attcacctat attccccttg aattttatct
     9721 ttaaaatcgt atttaatgta atttgagtta tctctatagg caagccaagt accttacttt
     9781 cttcataaat aaaatgttct acaatctttc ttttttcaat agtccctctt tcttctatat
     9841 tgggaacata aatatttatg ggtattcttc ttaaaaaagt ttgcaaaaat ttggatagac
     9901 tttctgttgt tgcaaatatt aatcttaccc tagcttttct aattactcca ttctctccaa
     9961 tcctagaaaa cgtacctgta tccataaatg taaataactt ttcctgacct tcactattta
    10021 atctatgtac ttcatctaaa aataaaacac caccatctga ttgttctatt agacctgctc
    10081 tagattcatt tgctccagta tatgctcctt ttacatgccc aaataataga ctagacaata
    10141 actccaaatt attataatac tgcgcacagt ttaacacatt gtatggagaa tttttttcta
    10201 atacattact gcttacacta tatttgtata ttatcttggc taaaaaagtt tttcctactc
    10261 cactttcacc tgtcagcatt attgtaagtc cgccatttgg ataaagtaca gcggttttta
    10321 tttgttcgat tacttctttt aagctattat catatcctat gatactatta aacacagtct
    10381 cttgctttaa taatttacac tcatttttaa gttcatctaa attttcataa atactttttt
    10441 ttgtaggtcc tactacatca tttattttat ttttaggtaa aaaaataaca ggtctactgt
    10501 ttatctttat aagattatca gattttacta aatcatttaa taatctactt gattgagttc
    10561 tacttaaatt aaactttttt actatatctt ctgttattat gccactttct tttttaagtt
    10621 catcaaagtt gttaaataat acacattcat ttacaaaact taaaatttca tccctcatgt
    10681 tcaacctcca atacaaatat caataatact ataatattag tatatcattt atatatttat
    10741 gaaaaagata attatattcc tatcttgatt ctcttttgta aggttgtaaa atcaaacttt
    10801 ttataagtat aaaatctaga ttttttaaca taatctcaag gttcttatct ttccatatat
    10861 ctgacataga atttatataa gaatatattt attgta
//
LOCUS       DAELXX010000122        10460 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.123, whole genome shotgun sequence.
ACCESSION   DAELXX010000122 DAELXX010000000
VERSION     DAELXX010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10460
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.123"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            254..1678
                     /locus_tag="KPH29_003117"
     CDS             254..1678
                     /locus_tag="KPH29_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HBG1468689.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANQSEEDSTSLEEAKAKMSDMKSASKNSEKNIEEKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLNIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDALFKRLESK"
     gene            1713..3842
                     /locus_tag="KPH29_003118"
     CDS             1713..3842
                     /locus_tag="KPH29_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBG1468690.1"
                     /translation="MKKKKITSRQKKIILMIVENSKKNIPITISEIAGTLELSSRTVL
                     RDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEERNLI
                     ILSKLLVSNEPVKSYYFAKILKVSEGVLNNDFALASKWLERFDIELVRKPGLGVYLKG
                     QEKNFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCIIRK
                     VEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITEEFK
                     LAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITNKMI
                     DLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYDGVE
                     KISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSRFLS
                     TKIENKFPNLNILETISAINIDEEYLKEKDVDLIVSTVELNTSLNYICVGPFMSLDDE
                     QIIKEKIKSIAQNKLINLNAKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEKFES
                     KDLSELIEDSSKIFAKSSEDIISIKESLKERLKISIPYIEESKILLLHCMSERIDIMK
                     LSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKINKF
                     SIEEMTLEVKDIVFNFYTNRLKAFIDK"
     gene            3872..4294
                     /locus_tag="KPH29_003119"
     CDS             3872..4294
                     /locus_tag="KPH29_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG1468691.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAN"
     gene            4313..5464
                     /locus_tag="KPH29_003120"
     CDS             4313..5464
                     /locus_tag="KPH29_003120"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG1468692.1"
                     /translation="MKKAIQFGAGNIGRGFIGSLLVKSGYHVVFADVNEEILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEMYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEVHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSIGVKKAVAKIANISNDEEILNNIEKSYIFMKNL"
     regulatory      5570..5671
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            5854..6426
                     /locus_tag="KPH29_003121"
     CDS             5854..6426
                     /locus_tag="KPH29_003121"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBG1468693.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKGEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            6490..7140
                     /gene="fsa"
                     /locus_tag="KPH29_003122"
     CDS             6490..7140
                     /gene="fsa"
                     /locus_tag="KPH29_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBG1468694.1"
                     /translation="MRFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHKGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGAFLKL"
     gene            7292..10216
                     /locus_tag="KPH29_003123"
     CDS             7292..10216
                     /locus_tag="KPH29_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG1468695.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKDPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYGTLIEVKPVAFLNNNLEEELNDIEAEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPALNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVIKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
BASE COUNT         4291 a         1014 c         1952 g         3203 t
ORIGIN      
        1 atttaactga attattattt tcatttctat taaatttaaa tattttataa aaatagtatt
       61 atttacaaaa tcatatactc caaaatacta cataaataaa attaaacaaa aactaaaatg
      121 acaaatgtca ttatctaaag tgacatagaa acggtttctt aaagttgtta ctataaaata
      181 taaactctat aataaaagta taacaaaagg gaatcatatc gatatagaat ataattcaag
      241 aggaggtaaa attatgagta gttctaatca atcgacaata aagacatcta tacagcgagt
      301 agggaaattt ttaagtggta tggtaatgcc aaatatagga gcttttatag catggggtct
      361 tataacagca ttatttatac caacaggatg gatgcctaat gaaaaattga gtactatagg
      421 cgacccaatg ataaaatacc ttttaccact attgatagct tatactggtg gtaaagcaat
      481 agcaggacaa agaggtggcg taataggtgc agcagcagct atgggagtaa ttgttggtgc
      541 agatatacca atgtttatcg gagcaatgat aatgggacca tttgcaggat gggtaattaa
      601 gaagtttgat aaatttgtag atggaaagat accaacagga tttgaaatgt tagtaaataa
      661 cttttcaata ggtataatag gtatgcttct tgcaatacta ggattttatg caattggacc
      721 agcaatagta gcagggacag ctcttataga atctggagtt caatttatag ttagcaaaag
      781 tttattgcct ctagtttcag tatttataga acctggaaaa gtattattct taaacaatgc
      841 aataaatcat ggtatattag ggcctatagg tatagctgag gcaaaagaag caggtaaatc
      901 tataatgttc ttactagaaa gtaaccctgg accaggttta ggagttctat tagcatattg
      961 gatgtttagt agaggaagtg taaaacaatc agcaccagga gctgtaataa tacatttctt
     1021 tggtggtatc catgaaattt acttcccata tattttaatg aatccagttt taatattggc
     1081 tacaatggca ggtggagcag caggaatatt gactttttca atattaggag ctgggcttgt
     1141 agcagcacca tcaccaggaa gtatatttgc acttatggca ctagctccaa agggaggatt
     1201 acttccagta ttagcaggag ttgcagtggc aacagttgta tcattcttag tggcagcacc
     1261 atttgttaag agagcttcag caaatcaaag tgaagaagac agtacttctt tagaagaagc
     1321 taaagcaaaa atgagtgata tgaaatctgc aagtaagaac tctgagaaaa atattgagga
     1381 aaaacagctt gaagtaaatg agattaagaa gattgtattt gcatgtgatg caggaatggg
     1441 ttcgagtgca atgggagctt ccagatttaa aaatagaata aaaaacttag acttaaatat
     1501 agaaattaca aattcatctg ttgataatct tccagatgat acacaaatag tagtaactca
     1561 caatacatta gttgaaagag ttgctaagaa taattcaagt gttgagatag tttcaataaa
     1621 caatttttta aatgacccaa atttagatgc attatttaaa agattagaat ctaaataaaa
     1681 ataaaaagta ccactaatta ggagtggata atttgaaaaa aaagaagatt acatctaggc
     1741 aaaaaaagat aatactaatg atagtagaaa attcaaagaa aaacattcca ataactattt
     1801 ctgagattgc agggacttta gagctaagtt caagaactgt acttagagat atgtctggaa
     1861 ttgaaaaatg gtttgatgaa aatgatttta actttgtaaa aaaaccagga gtgggattga
     1921 ttttagagga aaatatagaa aatcaaaact ttataattga gttgttagaa gaagaaaaaa
     1981 tagaaaaaga atacagcaag gaagaaagaa atttaattat attatcaaag ctactagttt
     2041 ctaacgagcc agtaaaatcc tattattttg caaaaatatt aaaggtatct gaaggggtac
     2101 tgaataatga ttttgcctta gcaagtaaat ggttggaaag atttgatatt gagcttgtta
     2161 gaaaaccagg gcttggtgtc tacttaaaag gtcaagaaaa aaactttaga gaggcatatg
     2221 taaatcttat atacgattca ttcaatgaaa aagaaatact agatatggtc agaaatataa
     2281 gtgaaaatat acaaacagat aaagcaatag aaattttaag tgaaaataga cttttaaatt
     2341 taatggacag gtgcataata agaaaagttg aagctacatt aacaaagaag ctttcagatt
     2401 tagatgtaaa tttagctgat agtgcatata tagggcttgt agttcatatt tcattagcct
     2461 tacagaggat aaaaaatggt gaaaatataa ctatggataa agagtttcta aaagaattat
     2521 ctataacaga agagtttaaa ttagctaaag aaatcgtaaa aggtatggaa acagatttta
     2581 acatggatat accagtagat gaggttggat atataactat gcatataaga ggtgcaaagc
     2641 aaaggtcatc ttcaaaccat aaggctttaa acttagatga tatagaaata atggaaatta
     2701 caaataagat gatagatttg gcggaagatg aatttaaaat aagtttaaaa aatgacgaga
     2761 ggttatttaa agacctagca aatcacttag ggccttctat aaatagattg aatatgggat
     2821 tagaaataag aaatcctctt ttagatgaaa taaaaagcaa gtattcatat gcctatgatg
     2881 gagttgaaaa gatttctaga attataaaag ataaactaaa tataaattca atacctgaat
     2941 cagaaatagg atatattgca atgcactttg catcagccat agaaaaaaat cttatgatga
     3001 atactaatat aaatattgtt gtggcatgtc ccactggaat aggtacttca agattcttat
     3061 caactaagat tgaaaataaa tttcctaatt taaatatact tgaaaccata tcagctataa
     3121 acatagatga agagtattta aaagaaaaag atgttgattt aatagtgtct acagttgagt
     3181 taaatacaag tttaaattat atatgtgtag gtccatttat gagtttagat gatgaacaga
     3241 taatcaagga aaaaataaaa tctatagctc aaaataaatt aatcaattta aatgcgaaaa
     3301 atgatactaa aagtaagaat aaagtatatg aacagataac agaaagtatg aatataggaa
     3361 aagatattct tcaattttta gaggaaatta gatttgaaaa atttgaaagt aaagatttaa
     3421 gtgagcttat tgaagactca tcaaaaatat ttgcaaaatc tagtgaagat ataatctcta
     3481 taaaagaatc gttaaaagaa agattaaaaa tctcaattcc atatatagaa gaatcaaaaa
     3541 ttctattgtt acattgtatg agtgaaagaa tagatattat gaagttatca ataataaaac
     3601 tagagaatag aatcgtgcta gattcaaatg aagaaatcga taatgtagtt tttatgttac
     3661 ttcctaaaaa ttcaccaagt tatcaaagac aaataatgag tgaaataagt ggttcattaa
     3721 tagacaatat aatttttact aataagataa ataaattctc tatagaagag atgacattag
     3781 aggtaaaaga tatagttttt aatttttata caaacagatt aaaagcattt atagataaat
     3841 aaaattaata ttaaaaagga aggttgatac tatgagtaaa gtattgaatg agaataatat
     3901 atttttaggg ttagatagtg tttcaaagga agaagctata actttagcag gaagaaaatt
     3961 agttgaaaat ggatatgtaa aagaagaata tataccagca atgttagaaa gagaaaaagt
     4021 aatgactaca tatatgggaa tgggagttgc tattccacat ggtgtaaatg aagcaaaaaa
     4081 agaaatatta tcttctggaa tagttatatt acaattccct aatggtatag attttgatgg
     4141 agaaaaagca tatttattaa taggaatagc tggtgttgga gatgaacatt tagaaatact
     4201 atctaatata gcagtagttt tagatgatga cttaacagaa agactaaaaa attcaaatga
     4261 caaacaagct tttatggaag cttttgccaa ctaatcttag gagggattta aaatgaaaaa
     4321 ggcgattcag tttggagcag gaaatattgg aagaggtttt attggaagct tgttagttaa
     4381 gtcgggttat catgtagtat ttgctgatgt aaatgaggag atattaaact caataaacaa
     4441 agataaaaag tatactatac acataagaga tgtagaatgt atagatgaag taattgataa
     4501 tataagtgcg gtttcatcta taaaagaaga aataatagat gagattgtac aggctgaaat
     4561 aataactaca gcagttggac ctttagtttt gactaaaata gcctctacaa ttgcaaaagg
     4621 tataaaagct agaaaagaaa agggattaac aagtaatcta aatataatag catgtgaaaa
     4681 tgcaatatac gcaagttctt ctttaaaaga agaggtactt aagtatttaa ataaggaaga
     4741 agtagaatat ttagagatgt atgttggttt tccaaactgt tcagttgata gaatagttcc
     4801 tcctggtaaa aacgaaaatc ctcttgatgt aacagttgaa aatttttatg agtggaatgt
     4861 tgaaaaacaa ggatttaaag gggaaatacc aactatagtt ggaatgaatt tagctgataa
     4921 ccttatggct tatatagaac gtaaattgtt tacactgaat acaggtcatg ctataacagc
     4981 ttatatagga tatttaaaag gatataagac tatagaagag agtataaaag ataaatttat
     5041 ctgtgatata gtcaaaagtg caatggtaga aagtggagaa ggattgatta aaaaatacaa
     5101 ttttgactca gaagtgcact ataaatatat tgataaaatt ttaaatagat ttaaaaatcc
     5161 atatcttaat gatgatgtat taagagtcgg aagggaacca ttaagaaaac taagtgataa
     5221 agatagattg attaaacctt taatgacagc aaaatcatat ggattatcag ttgacaattt
     5281 gattcttgga ataggtgcag cgcttcatta taataatagt gaagatactc aaagtgtaga
     5341 attacaagag ttgataaagt ctattggggt aaaaaaagcc gttgctaaaa tagctaatat
     5401 aagcaatgac gaagaaattt taaataatat cgaaaaaagt tatatattta tgaaaaattt
     5461 ataaatttac ttgaaaaaaa gtagttctga taaagtaact ataatatatt attctacaca
     5521 gtttgaatta tttgttgaat aatagagaat tataatatat aattaaaagg aataaattaa
     5581 tatatttacc tgtatatgtt tgataatatg gttcaaatgt ttctactaag ctaccattaa
     5641 tagcttgact acaggagtga agaagatata gtttatactt attttaaatt atatattaag
     5701 ttaattactt tgaattttaa gctcctgtct atgatataga caggagcttt tttagtattt
     5761 taatataaaa tacttacaag aatagatagc aaaattagaa caaatataat ttttttatta
     5821 atataatttt agaaagggct aggtggaagt tcaatgaagg ctttacaaga aaagatattg
     5881 agagaaggca gtgtttcagg aaatgatata ctaaaagttg atagtttttt aaaccatcaa
     5941 attgatgtag cttttttaaa tgaaatagga agagaattta aagagagatt taaaggagaa
     6001 aaggtggata agatatttac aatagaggct tcaggaatag ctatagcatc aattgtttca
     6061 cagtattttg ataatgcacc tgtagtattt gcaaaaaaat cagaatctaa aaatttggat
     6121 aaagatgtat atgaaactaa tgtatattca tttactaagg caagagagta ttcagttaaa
     6181 gtttctaaaa agtacataaa caaaggcgaa aatatattga tagtggatga ttttttggca
     6241 aatggaagag ctgctcttgg acttaaagat ttaattgagc aagcagaagc taacttagtt
     6301 ggagtaggaa ttgtcattga aaaaggattt caagctggag gagcactttt aaaggctaat
     6361 gatgtaagat tagagtcttt ggcagtagtt gaatctatag ataatggaac tgtaaaattt
     6421 aggtaattta tgatattata taattgtgtt tagcacatat tttaactatg taattatgga
     6481 ggatttttta tgaggttttt tatagacaca gccaatattg aagaaataaa agaagcaaat
     6541 gatttaggtg taatatgtgg tgtaactact aatccgtctc taatagctaa agaaggtaga
     6601 gattttatag aggtagttaa agaaataagt gaaatagtag atgggccaat aagcgcagaa
     6661 gtaataagtt tagaacataa aggtatgata gaggaagcag aaaaattatc taagatacat
     6721 aaaaatatag ttataaaaat acctatgaca gcagaaggtc taaaagcggt gaaagtacta
     6781 tcaagtaaag gtataaagac taatgtaacg cttatatttt ctgctggaca agctctttta
     6841 gctgctagag ctggagctac atatgtaagt ccttttgttg gtagattgga tgatatatca
     6901 caaaatggtc ttgatttaat tgaagaaatc gttgatatat ttagtgtaaa tagtattgag
     6961 gctcaaataa tagttgctag tgttagaaat ccaatacatg ttttacaagc tgctagaatg
     7021 ggagcagata tagcaacagt tcctcttaaa gttataaacc aaatgattaa gcatcctttg
     7081 acagacaaag gaatagatag ctttatgaag gattgggaag gtgctttttt aaaattataa
     7141 ttataaacta cgctttataa gtatattaaa gaggttatta tggaaattct aagtaataag
     7201 gctaggctat tttgcctagt cttattttgg tgtataatat attgaaacat attattgaaa
     7261 tagattaatt ttttaagtga ggaggcgaaa aatggataaa atattaagag agatacaaag
     7321 agaagataaa aaaaatccat atactgacca agaacttgct gaaatgttaa atgtagctag
     7381 gtctgaagtg atatcagtta ggaaaaaaaa taatatttta gattcgagag aaagaagaaa
     7441 aaaaatatta ataaaagata tatcaaaaat tgtaagtgaa aatcctcaaa tgtctgaaag
     7501 aaaaataact gaaatattaa tagaaaatgg atataatata tcgagaagtg cagtttcaaa
     7561 attattaaaa gaggaaaatt taaatcagtc aaagattggg attaaggata aagacccagc
     7621 atcatcagga aagtatgtga aggtgagtaa agttgtagaa gaagatggat ttgaagaatt
     7681 gataggtata aagggaagtt tgaaagaaaa ggtaaatcta gctaagtcag caataatgta
     7741 tcctcctaat ggccttcata ctataatata tggagaaaca ggagtaggta aaagtgaatt
     7801 agctacatgt atgtataaat atgctataaa aaacaacatt aaagaagaga acagcccatt
     7861 tatagtgttt aactgtgcag actatgcaga aaatcctaat ctattgatag ctcaactctt
     7921 tggggtagta aaaggtgcat atacaggagc agatgcaaat agagaaggtc ttgtagaaca
     7981 agctaataat ggaatattgt ttttagatga aattcataga ttgcctgctg ctgggcaaga
     8041 aatcttattt tcattaatag atagaggaga atttagaagg ttaggagaaa gtagttctaa
     8101 tagaaaggca aatgttctca taataagtgc tacaacagaa aatccagatt ctaatttact
     8161 tcaaacattt agaagacgta taccaatggt aataaattta cctaatatga gtgaaagacc
     8221 aaaatctgaa agatatgaaa taataataaa gttttttgaa agagaagcaa agcgagtaaa
     8281 caagaatttt ataataacta aagaagttat gtgtgcactt atgaattata agtgtactgg
     8341 aaatataggt cagttaaaga gtgatataca agtaacttgt gctagagctt ttagtaaatc
     8401 gatattttct agcgataagg taattataga tttagatagt ttaagggatt atataaaaag
     8461 tggatattat gattgtaatt gtaaaaatga aaagtattct gagttttcag ttgaggatat
     8521 atatatagat attagtgaat tggtgagtgg aaaaaataga attttatctg ataatgagat
     8581 aagcgagata tataattatg cagaaaaaga gcttaaggtt ttggaaagta agtgcttttc
     8641 agaagaagag ttaaaaaatg catttataga aaagttagat aagaagttta atgctattaa
     8701 aaacaataaa aatttatctg atagaagaag tcgaatagac tggggaggac aattaaaaga
     8761 gtctacactg gagataatgg ataaggtaat tgtatttatt aaggagcagt ttaagcatgt
     8821 aaatcaaggt ctgtacttgg ctctagcaat tcatattgag catgctatag gtaggataaa
     8881 agatggaaaa actataataa atccgagttt agataaaata aaagcatcaa tgccaatgga
     8941 atatgagttg tcaagatata taatgggtat agtagaagat ttgacacaag taacatttcc
     9001 agaagatgag cttggatatt tggcttatta tataaataaa ttttgttata atgaggaaag
     9061 tataaaagat aaagttaaag tagtaatagt tacgcatggt aaagttggaa ttgaaatgtc
     9121 taaagttgta aatcatattt taggtataga atgtacacta ggtatagaaa tagcacttac
     9181 agattcacca tcagagggga tagagcatgt attagaagag cttcaaaaga tagaagctag
     9241 aaaaggtatt ctagttctaa ttgatatggg ttctttggta attcttggtg atgaggttga
     9301 aaaaaggctt ggaattagat gtaaaactgt aaatagagta gatacattat tggcaatgga
     9361 agcaggaaaa cttgcaacca ttgaaggaaa aagtttagat ggtataatag ctgatttaaa
     9421 gaaaaataag aattatgcta tgataaatac aaataaattt agttacagaa aaaacgagta
     9481 tggaaagaaa aatgtaatca ttactttatg tttgagtggt gtaggtacag ctttaaattt
     9541 aaaagagcat atagaaaaac aaattaagga atatggcact ttaattgaag taaagccagt
     9601 tgctttttta aataataatt tagaagaaga gttaaatgat attgaagctg aatataatat
     9661 tattgctatc tgtggaacta ttgatataga ttataaaaac gtgcctttta tttcttataa
     9721 tgaggtactt ggtaaaaatg gtattaataa agtattagag catctaaata gtaaacaacc
     9781 agctttaaat attgaaaacc agttgaataa tttaatacat gaagatttga ttttatacaa
     9841 ttttgaagga atttcaaagg agtatataat tgatactctt gtttcaaaac ttgaagaagg
     9901 tggatatgtt gattcaaagt atatattaag tgtatataaa agagaagcaa tgggaagtgt
     9961 tgtaatggcc tctaaggtag ccgtgccaca tggacttcca gaaaacgtta taaagcctgc
    10021 aatagcaatc gcaaggttga ataaacctat agtatgggac aataaattta tggtggattt
    10081 ggtggtacta ctggcactta aagaaaataa taagaaagaa atacgaagct tattttcaaa
    10141 gattaatgac cagcatactt tagaagtttt attaaatact gaagataaaa atgaaataaa
    10201 agaactttta agctaattat tagtaccgag aagaaagaca aaaaaatttg ctttagtata
    10261 aaatttaaaa ttactcaatt gctaagtagt taattactct atataaggct agttctatga
    10321 aatttattta tatgatttta tagaattagc ttttttcttg gtaaataaaa tatatgaatt
    10381 tatttatgta aaaagtaaat tttattggac aattctatga aaatttttcc tatatttctt
    10441 ataaaatata gaatatataa
//
LOCUS       DAELXX010000123        10368 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.124, whole genome shotgun sequence.
ACCESSION   DAELXX010000123 DAELXX010000000
VERSION     DAELXX010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10368
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.124"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            296..508
                     /locus_tag="KPH29_003124"
     CDS             296..508
                     /locus_tag="KPH29_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG1468696.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            521..742
                     /locus_tag="KPH29_003125"
     CDS             521..742
                     /locus_tag="KPH29_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG1468697.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            842..2998
                     /gene="feoB"
                     /locus_tag="KPH29_003126"
     CDS             842..2998
                     /gene="feoB"
                     /locus_tag="KPH29_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBG1468698.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTIIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHVPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            3024..3176
                     /locus_tag="KPH29_003127"
     CDS             3024..3176
                     /locus_tag="KPH29_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBG1468699.1"
                     /translation="MATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            3356..4066
                     /locus_tag="KPH29_003128"
     CDS             3356..4066
                     /locus_tag="KPH29_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBG1468700.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            4151..4921
                     /locus_tag="KPH29_003129"
     CDS             4151..4921
                     /locus_tag="KPH29_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1468701.1"
                     /translation="MFEKIHKRFKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVVFGVNKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            4914..5645
                     /locus_tag="KPH29_003130"
     CDS             4914..5645
                     /locus_tag="KPH29_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1468702.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNIYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            5679..6638
                     /locus_tag="KPH29_003131"
     CDS             5679..6638
                     /locus_tag="KPH29_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG1468703.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(6736..7332)
                     /locus_tag="KPH29_003132"
     CDS             complement(6736..7332)
                     /locus_tag="KPH29_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HBG1468704.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVNKLKSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
     gene            7578..8177
                     /locus_tag="KPH29_003133"
     CDS             7578..8177
                     /locus_tag="KPH29_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468705.1"
                     /translation="MKLKRTLFVGLALVLAIGITACARDNEKPNENAGKNPNESVNNN
                     EGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRD
                     KIQSFVTSLKKDGKTEDTELKRMNDDLIAEGERLVKDIDERIEKLDKVSDEDMKKGQN
                     DFMKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
     gene            complement(8345..8812)
                     /locus_tag="KPH29_003134"
     CDS             complement(8345..8812)
                     /locus_tag="KPH29_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468706.1"
                     /translation="MENDTIKADDILNYCLSNLDDVVLMDSWGERAIYYNPNGVLKRG
                     VYVLTIKEKDSNHDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICVLSPTEKTFENFKTLIEESYNFAKQKFKKRKNK"
     gene            8923..9834
                     /locus_tag="KPH29_003135"
     CDS             8923..9834
                     /locus_tag="KPH29_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBG1468707.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SVGFPLYSKVGAAGGYHVLKERILPPITFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENMRRKLVFWTPDRHCSTPLLKELFNIVMNEFTIKIEYSSK
                     QKNSIRTIVPIGLYAMNGLWYCPAYCIKSESVKEFRADRIVKILSIENLSNKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKRCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHLIKK"
BASE COUNT         3937 a         1147 c         1876 g         3408 t
ORIGIN      
        1 tgcagaatat ggttatgagt atctaagaga aatctcaaaa tatataggat agcagtatga
       61 atttttatag attttattaa tacaataaat caaatgagaa aaaaaatcaa aagaatacaa
      121 taatcacttg caaaaaatat gctattgtga tagacttaaa tcaaaattag gaattgtaag
      181 aataaggaaa aagataagta attattttaa cgagtcaaaa tttaagaaat tgtgatttat
      241 atataagata gaggttgttt gtaaatttaa attggttgaa gaggaggaat attatatgat
      301 gccattgata ttatcaaata caggtgaaga agtagttata aagaaaattt cgggaagtga
      361 taagacgagg acttttttaa atagtatagg ttttgtagta ggtacaaatg ttaaaatagt
      421 ttctaagata gatggtaact taataataga cgtaaaagat actagagttg cattagataa
      481 aaaaatggca agtagaataa taatataggg aggtaatgac atgaacagac tgaaggatat
      541 taagtgtgga gagactgtaa aagtcaaaaa gttagaagga gaaggagcta cacgaagacg
      601 tataatggat atggggatta caagaggtgt taatatattt ataagaaaag tagctcctct
      661 tggtgatcca attgaggtaa cagtcagaga ttatgaacta tcaattcgta agtcagatgc
      721 agaaaaaatt attgtggaat aataaagatt aattagagta gggttaatgt aattatataa
      781 tgagaatgat tttctgtatc acaaacagtg atgtatttaa ataataatgg agggaatgga
      841 aatgtcaata aaaataggtc tcataggaaa tcctaactgt ggtaagacaa caatgttcaa
      901 tgggcttaca ggctcgtcgc aatatgttgg taactggcca ggagtaacag ttgaaaaaaa
      961 aggtggaaaa ttaaaaggaa ataaagatgt ggaaatagta gatttaccag gtatttattc
     1021 tttatctcca tatacattag aagaagtagt tacacgtaat tttatgctag atgataagcc
     1081 ggatgcagta ataaatattg tagacgcatc aaatatagag agaaatttat atcttacaac
     1141 tcaagtttta gagctaggta taccgaccat tatagctctt aatatgatgg atattgtaaa
     1201 taagaatggt gataagataa atattaaaga actatctgaa gttataggtt gcccagtggt
     1261 agaagtaaca gcagttaaag gtcaaggaat tatggaggct gctgaaaagg cagttgaact
     1321 tgcaagtagt aacaataaat taaatttcaa attacctttt gttgatgaaa gtaaggacgc
     1381 aatagaaaaa attgaaaaaa taatagaaga aaagactcca tatatagatg tagaaacacg
     1441 ctggttagct ataaagctat ttgagagaga tgaaaatgtt atacaaaaac ttgatatttc
     1501 taaaacaata ttgaatagta ttgaagaaat aactagaaat tgtgaagatg aattggatga
     1561 tgatagtgaa agtataataa cagccaatcg ttatgaattt attagttcaa taatttcaag
     1621 tataataaag aagaatcgaa aaggtaagga aactgtatca gataagatag ataagattgt
     1681 aacaaatcgt atactagcat tacctatttt tgcacttata atgtggggag tttactatat
     1741 agcagtaagt tcattgggta ctatagcaac tgattggaca aatgatgtac tgtttggaga
     1801 aattattcaa ggcaatgtaa gtaattttct agcttcttta aatgtagcag agtggttaca
     1861 aggacttgta gttgatggtc taattggtgg agttggagca gtactaggat ttgtaccaca
     1921 aatcatgctt ttattcttat tattgtcaat attagaagat tgtgggtata tgtcacgtgt
     1981 agcttttata atggatagaa ttttccgtaa gtttggtctt tcaggaaaat catttatacc
     2041 aatgcttata agttcaggtt gtggagttcc aggagttatg tcaactcgta ctatagagaa
     2101 tgatagagat agaaaaatga ctattatgtt aactacattc attccatgtg gagctaagat
     2161 tccaataata gcattatttg caggggcatt atttggaggt gcttcatggg tagctccatc
     2221 aatgtatttc ttaggtattg caatgataat catttgtgga attatattaa agaaaactag
     2281 tttatttgca ggagaaccat ctccatttgt tatggaatta cctcaatatc atgttccaag
     2341 tgcaaaaggt gttttaatac atatgtggga tagaggaaaa gcatttataa tcaaagcagg
     2401 aacaattata tttgttgctt gtggagtaat ctggttctta caatcattta attggtcatt
     2461 acaaatggta gatgctggag atagtatatt agctagctta ggaaatatag ttgcaccaat
     2521 atttgctcct cttggatttg gaaattggca atcatcagta gcaacagtta caggtctagt
     2581 agctaaggaa aatgtagttg gaacatttgg agttttattt ggtatatcag atgcaacaga
     2641 gcaagaccca acacttcttg cttcagttgc aagtatgttt acagtagcta gtgcgtttgc
     2701 atttatggca tttaatatgc tttgtgcacc ttgttttgca gctattggag ccataaaaag
     2761 agaaatgggt tcatggaaat ggacatggat tacattagga ttccaaactt taacagcgta
     2821 tataattgct cttcttataa atcaagttgg aagtttagtt ttaggtactg gaggaagtat
     2881 tgctggggca ataatatcta tatttatagc agtagcagta gtctttgtag ttttaactta
     2941 ttcaaataaa aatatgaaaa aagaaaaaat gggtaaatta agttatatga aaaattaatt
     3001 catataaata aggagtgatg aatatggcta catttattat agcagctatt gtagtagtat
     3061 taatggcact agctgtaatg tatatggtta aaaattcaaa aaagaatggt agcagttgtg
     3121 gatgtggttg ttctggatgc tcaagtagta agagttgtca tagcgctaag agataaatag
     3181 gataagagcc tgataaaaag ttaaattgct ttattatcag gttcttattt tttatataaa
     3241 taacaaattt catatttgaa gtaggttaga ctatgctgta attttactgt ataattatat
     3301 ataaatatat gattaggagg gattattgct aaattaagca atataagtga atattatgtt
     3361 taaatttgat gttacaaacg agcttatgtg tatagcaagg ggaagtggta aattttttgc
     3421 aaaaaaaggt gctatggtag cctttaaagg aaattttaat tttgaaaaat tgctgttagg
     3481 tccaagtaat ggcggtggat tgggaagagc attattaggt catgttcaga ggtcattgac
     3541 aggagaacaa atgccaataa tggtggttga aggtgaaggt gaaatttacc tagctcaaaa
     3601 tgcttaccat gtagatgtaa tatcaattga cccaggtgat tccatttatg ttgagagtga
     3661 aaacttattg gcattttctg aacaattaaa ttacagtgta aaattgattg gttcaggggt
     3721 aatatctcaa aaaggtcttt ttactacaca tttagtaaat aaaacaggtc aaaatcaaga
     3781 tgttgctata ataacagatg gtaatcctct aattcttgaa ggaccttgtt gtgttgaccc
     3841 agatgcatta gtagcttgga ctggaagaga accatatcct aaagtatcaa aactatcatg
     3901 gaagactttt attggtcaga cttcaggaga atcttatcat atggaatttg ttgaacctgg
     3961 acaaatagtc attatacaac catctgaaag attgtctggc ttaaaaacag attctacacc
     4021 ttctgataga atgagttcag gtggattaag acttggcata gattaaatct tatgatatag
     4081 cttaattatt atttaagata ttaaaaaata aaaagggtag cagggataga tatgttaaaa
     4141 ggagttttat atgtttgaga aaattcataa aaggtttaaa ggatattata tttttttaat
     4201 aattttaatt ttatggaaga tagtatgtgt tttgggtata tggagtagct atattctgcc
     4261 tcctcctgaa atagtcttag atacttttat aaaaatgata tatgacggtt ctattttctt
     4321 aaatgtttat gccagtgtaa gaaggatatt aataggtttt ttaataagtt cattgatggc
     4381 tataccactt ggagttgtat ttggagtaaa taaaaaaatg tatgaatatt tcaaaccact
     4441 atttgaattt atgcgaagta cacctccact agctttggtg ccaatgctta ttttatggtt
     4501 tggtataggt gaagaatcta aaattataat tattattttg gcatcctttt ttccaatatt
     4561 tttaaataca ttgaaaggta taaaaaattg tgacaataaa ttaatagaag taggtaaatc
     4621 ttttaattta tctaaaaaga gaatttttta taaaataata atccctaatt ctatgctgga
     4681 tattgttgtt ggacttagtt tagggcttgg gtatagtttt agggctataa ttggagcaga
     4741 gcttattgca gcttcttcag gactaggata tttgatttct gatggaaaag atatgtctag
     4801 aactgatgtt gtattggtag gaatttttgt tattggattt ttaggtatac tttcagatta
     4861 tctattttca aagataataa caaaatttag caaagggaag caggttgatt taaatggtta
     4921 gaagtggatt tttgctagag aatatatata agaaatattt agttgataat aaagagcatt
     4981 tagttcttga taatatatct ttaaatatat ctagtgaaga gataactgtt atattaggtg
     5041 aaagtggatg tgggaaaaca acgctactta gaattttagc agggcttgaa aatgctacta
     5101 gtggaaacat atattttttt aataatgata aaaagtgtac tccaaaagta ggaatggtat
     5161 ttcaagaaag tagacttatg ccatggctga atgtaagtga aaatatactt ttacatactg
     5221 aaaaagataa tagaaataaa gttgatttag acaagtattt aaaaatgatg aagcttgaaa
     5281 agtttaaaaa ctcatatcca aatgaattat caggaggaat ggctcacaga gtaagtatag
     5341 caagagcact ttccttcaat cccgatattt tgctgatgga cgaaccattt gcagcgctag
     5401 attacttcac aagaagaaaa atgcaaaaag aagttgtaaa tatccataaa aatactaaaa
     5461 aaggtgtagt atttgtaact cacaatattg aagaagctat ggaaatagca aagaaaataa
     5521 ttgtttttag taaaaataaa aggattaaac aatttagtgt cgaagatgaa tataatagag
     5581 atttaactaa aaactattat ataaacttga aaaaagaaat attaagggaa ttgggggagt
     5641 tttaattgat taaaagaaac aaaaagattt tagcagtaat gacatcttta ataatggtgt
     5701 tggttgggac tgtaggatgt tcaacaacaa atgataagaa agatgaacct gcaaaaaaag
     5761 aggctagttt acctaaggaa ataaatttaa cttatgtaaa atcaccactt aatgttccta
     5821 gtatattaga aaaaaaggat gatttatttg gaaaggaatt taaaaaggac aatataaaag
     5881 tcaacttcca tgaattgaca acaggaccag agcaaactca agcattagca gcaggtgagc
     5941 tagacttttt acatgcatta ggaggaacgt ctgcaataat agctgcatca aatggtgttg
     6001 gtcttaaaat tacgaatatt tatagccgtt caccaaaagg atatatgctt ataactaata
     6061 atgataatat taaatctata aaagacttaa aaggtaaaaa aattgtaggt ccaaaaggga
     6121 ctatacttca tcaactgttg attgctgcac ttgctaaaga aggctatact atagatgatg
     6181 ttgaatatgt aaatatggat attcctagtg ctgcttctgc attagaaagt aaaagtgctg
     6241 atgtagcaat gcttgcaggt ccagtggcat tgaaaacgat aaattcaggt gccaaaatga
     6301 ttgtaaatgg agaaggatta gtaagtggaa taatagttac tgctgtaagt gatgactttg
     6361 ctgaaaaata tcctgaatta gtaaaaagat ttatgaaggt tcatgaagaa actttaaaat
     6421 atatgaatga aaacaaggat gaagtgatgg atgttgtatc taaagaagtt ggtttatctt
     6481 tagatgaaac aaaagaaatg tattcttggt atgactttag ttctaaaata acggacaagg
     6541 atataaaaga attagaagat acacaggaat ttttgatgtc taatggaatg caacaaaaga
     6601 aaataaatat taaagatatg ttgtacaatc aaaattagaa tactttaaaa tattaaagtg
     6661 gaaaatacac ccttatttat tatggaaact gtatggtaaa agagggtgta tttttattat
     6721 tatacaaaaa tacaattaaa aacaacttac tattttatct acacttatgt agtctagtaa
     6781 ttttctcatt ttcttacggt gaagtaaaaa ctttgaatat gtgtcctcgg attgttttgt
     6841 gtcaccataa actttccaat atttaatcat agaaacttca taattactat atcccataaa
     6901 tccaatcaca tctttaagag gatatcctaa aaaaatacct atttcatcag gaaaaacatc
     6961 actcttaagt ttatttacta aatgctctaa ataagcatta acattcgtat tttgtttgta
     7021 tcctaaaaac tttaaaaagt ttactgtttt tttacatttt aatttttctg ataatgcttt
     7081 tttatttaca aataagattt ttaatccctt tttttcttta tcaataacta taaaatctat
     7141 tttcttacat ttatatagat atttataaat atcattaatt ttttcctctc tatttacatc
     7201 agtaaacgaa atatttatta tttcagcagg tttagagcct aaaaagactg gacctaaaac
     7261 ctctaaaagc cattttatat atgatgattc aattttattt ttacaacaaa attgtgaatt
     7321 acaacaagac ataaaagata acctccttca aatgaaaatt aatatcaagt aattagatta
     7381 tactatgaat gataatcaat atcaataata aaattataat ttttttattt aattatttat
     7441 tcattatgat ttccaaaaaa ataaattata acttgtttaa ttttagtaaa taaaaaataa
     7501 aaatatagaa atactaaatt aagaaagaaa attattaaaa ttttttgtag tacaaaacat
     7561 ctaggagggt aataaaaatg aaattaaaaa gaactttatt tgtaggatta gcattagtat
     7621 tagctatagg aataactgca tgtgctaggg ataatgaaaa accaaatgaa aatgcaggaa
     7681 aaaatcctaa tgaatctgta aataataatg aaggatatat gaattattat tcaacaagtt
     7741 ataatgatta tatagcaggt ctaaatagat attctattta tgatactcca gaaagtataa
     7801 acaatcagtt taaagataaa gaatatccag gaaatgaaaa atatctaagt gatgtaaagg
     7861 ctgcatataa agatagtaga gataaaatac aatcatttgt aacatctctt aaaaaagacg
     7921 gtaaaacaga agatacagag ttaaagagaa tgaatgatga cctaatagct gaaggggaaa
     7981 gattagtaaa agatatagat gagagaatag aaaaattaga taaggtatct gatgaagata
     8041 tgaaaaaagg tcaaaatgat tttatgaaac tagttcatgg aactgaagat gttggaaacg
     8101 atattggtag tggttttaga aaaatgataa aagatatgaa tgatagattg ggaataacag
     8161 gaaataataa aaaataatag agtaaatagt tttaaagtta gaaataaata tttgaaatat
     8221 agcgaagcct tgtaattaat atattttttg gcttcgcttt tttgtatgtt atcaagatac
     8281 taatttttat cttgtaacct catattttca ttatagttta tttagaggtt atttttttat
     8341 gtttttactt gttttttctc tttttaaatt tttgttttgc aaaattgtaa gattcttcta
     8401 ttaaagtttt aaagttctca aatgtctttt cagtagggct taggacgcat atccatccca
     8461 tccatgagta gataggatgt ggcatgattg tgtccaattt tgtaaaatca aaatccatat
     8521 caactatttg ccctacacct ggacgctttg gaatatatcc aaacatttca ataaaagttt
     8581 ctttttttaa tcctatattc acacggtata catttggacg actaaccaac gaacctttat
     8641 catgattact gtctttttct ttaatggtaa gaacatatac ccctcgcttt aaaacaccat
     8701 taggattgta gtaaattgct cgttcccccc aactatccat tagtacaaca tcatctaagt
     8761 ttgatagaca ataattgaga atatcatcag ccttaatagt atcattttcc ataaaatatc
     8821 aatcccttct ttctttaata ttattattgc tttagatgca agaatatctt atcatataat
     8881 ggtgacaatt attgtcacta tatttaaatt aggagtatga atatgtcaag agctgagcgt
     8941 ttaattgaac tgatgataac aataaatgca aaaagaagtt ttacagcggg agagctagca
     9001 gaagaatttt ctgtatcaaa acgtactata ctaagagatt tacaggtatt ggaaagtgtt
     9061 ggatttccac tatattcaaa agttggagcg gctggaggat atcatgtact taaagaacgt
     9121 atacttccac caattacatt ttcagagagt gaggtaaaat ctatattctt tgcatatcag
     9181 tcactagagt attataatga tttaccattt gagcaagaaa ctatttctgt tttaaagaaa
     9241 tttttaaatt gtataccaaa tgatattcaa tataatattg agaatatgag acgtaaactt
     9301 gttttttgga caccagatag gcattgctca acaccattat tgaaggaatt gtttaatata
     9361 gtaatgaatg aatttacaat taaaattgaa tattcatcaa agcaaaaaaa tagtatacgt
     9421 accattgttc ctattggttt gtatgctatg aatggacttt ggtattgtcc agcttactgt
     9481 ataaaatcag aatctgttaa ggaatttcgt gcagacagaa ttgtgaagat tttgagtata
     9541 gaaaacttat caaataagaa atataaggtt ttatcttcta tacatgatta tcttaagaat
     9601 atggaagtag gtacagatta tcatattaaa attaatctga cagatgaggg agtaaaacgt
     9661 tgtgaaacag aatttttgtt ggcaagagga ttgaaaattt tgtctaaggg tggatacata
     9721 gatatgtata taccaaaatc tacacttaat tgggtggctg aatatttttt aacttttgga
     9781 aaaaatgcta caataataga acctatagaa cttaaacatt tgattaaaaa atagctacgc
     9841 acacttgtga ctttagtcat gagttagtag ctaaattagt cagcatatag ggaaacttat
     9901 atgtagagat aggataacac ctatccaata ctacttgaat tgctaaaaac ccctaaagct
     9961 aaccaaacta aaacataagg atgaaataaa cctaagtgtg aaagttacga aagtagaaaa
    10021 aattggttag atggtataag gttaaatcct aagtacttta aaatgggcaa tcagcaggta
    10081 agctccgaat aggagaaact tcaacgacta ttcctcttga gggaagtaca ctacaagtta
    10141 tcggtagtgg aagtgggtag accttagcgg ataatgccga aggataagat atagtctgtg
    10201 cttgcatgaa agtgtaagaa gttcataaga gaactgcata ggtactagcg aacttatgtg
    10261 aacgacaacc tcaaaaatga taaaagttaa gacttacggt tcttatatat gttatttaaa
    10321 aataaatata aaaaaccttc aaaatacttg ttttaagtaa ttgaatat
//
LOCUS       DAELXX010000124        10360 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.125, whole genome shotgun sequence.
ACCESSION   DAELXX010000124 DAELXX010000000
VERSION     DAELXX010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10360
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.125"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            127..861
                     /locus_tag="KPH29_003136"
     CDS             127..861
                     /locus_tag="KPH29_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG1468708.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGNFVFSVEHPIFTAYGSQEWYCDK
                     DGNNLHWPVDRYFEEGVRKSNFLGEEVIKYHKTLTTYLNTLMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            complement(1003..1494)
                     /locus_tag="KPH29_003137"
     CDS             complement(1003..1494)
                     /locus_tag="KPH29_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBG1468709.1"
                     /translation="MNNQQQEVYKKLDELSIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
     gene            1843..2226
                     /locus_tag="KPH29_003138"
     CDS             1843..2226
                     /locus_tag="KPH29_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468710.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKVVLKSPEYIGSHPKKPGKSVEFIKKIDNYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNNGRVKQYDG"
     gene            2732..3115
                     /locus_tag="KPH29_003139"
     CDS             2732..3115
                     /locus_tag="KPH29_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG1468711.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            3117..3467
                     /locus_tag="KPH29_003140"
     CDS             3117..3467
                     /locus_tag="KPH29_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468712.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFLDKK"
     gene            complement(3561..4019)
                     /locus_tag="KPH29_003141"
     CDS             complement(3561..4019)
                     /locus_tag="KPH29_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468713.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLNEVYINQSRASYISGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(4064..4852)
                     /locus_tag="KPH29_003142"
     CDS             complement(4064..4852)
                     /locus_tag="KPH29_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG1468714.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQGGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            complement(4991..5428)
                     /locus_tag="KPH29_003143"
     CDS             complement(4991..5428)
                     /locus_tag="KPH29_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HBG1468715.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKDCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            6250..6759
                     /locus_tag="KPH29_003144"
     CDS             6250..6759
                     /locus_tag="KPH29_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1468716.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVESEVDSLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVIVIVFVVLGGLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLVPIVLIGAII
                     GIFLTRKKI"
     gene            complement(7670..8470)
                     /locus_tag="KPH29_003145"
     CDS             complement(7670..8470)
                     /locus_tag="KPH29_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1468717.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(8665..10263)
                     /locus_tag="KPH29_003146"
     CDS             complement(8665..10263)
                     /locus_tag="KPH29_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG1468718.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGVKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFSYLVCYRLDRISRNVSDFSTLIEKLNELNISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNDEQMEIVKLIYNKYLELRSLSKLYKYVYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILDYLKNSGMDVVGTPDRKCGILTYAKNTSNSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRISTGKTALLSGLIKCGKCGSNMRITYKGK
                     KTDKDLKYYYICGTKKTLGSDSCNCKNLNGPLVESLVIDKIKNCKEESIISAFNSNNI
                     KTDNVFNIFKSETNLLKNQILDKERLIKNLVIELAKNTGSTASDYIVSQIESLNNEIS
                     ILKDKINILESSNINISSDSINIDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIIW
                     DSSLGNLNIVYMGAEVDNSVSLSN"
BASE COUNT         3770 a         1489 c         1335 g         3766 t
ORIGIN      
        1 tcatttatat tattaaaaaa tttgtttata gaaactttta ttattttata taaaaatgat
       61 aaaaacttat attttgtatt taatagagtg ttgctaagtg aatatattta aaatggagga
      121 aactttatga aacaaaataa atatgatgat gatagatttt ttaataaata cagtaaaatg
      181 gaacgttcta caaatggatt agcaggtgct ggagaatggc atgttttaaa aaaaatgtta
      241 cctgatttca aaggaaaaag agttttagat ttaggatgtg gatttggatg gcattgtcaa
      301 tatgcagttg agaatggagc aatttctgct gttggagttg atatttcaga aaaaatgtta
      361 aaagaagcta gaaacaaaac gcaacttgat aatataaatt acatatgtat gcctattgaa
      421 gatattaatt ttccaaaaga tagttttgat gtagttatta gttccttggc atttcattat
      481 attcaatcat ttgaagatat atgtaagaat ataagtaatt gtctttcaaa taaaggtaat
      541 tttgtatttt ctgttgaaca tcctatattt actgcatatg gaagtcaaga gtggtactgt
      601 gataaagatg gcaataatct acactggcca gtagatagat attttgaaga aggagtacgt
      661 aagtcaaatt ttctaggaga agaggttata aaatatcata aaacattaac aacttattta
      721 aatacgttaa tgaaaacagg atttgaaata ttagaaatta ttgaacctca accagaagaa
      781 aaactgttag atacaattcc tgatatgaaa gatgagctac gtagacctat gatgttactt
      841 gtatctgcaa aaaaaagata agtaaatata aataatcatt attgtagaaa aaaattgtag
      901 aaaataatta cactaatgga gatgattata ccagtggtat taattacacc agtagtaagg
      961 aggcaaaagc attcgttgct tttgccattt tttaagttgc aattatactt ctacgtagta
     1021 tatttcgttt ccattttctt ctataagctt tttcatatct tcaaatttta aaaatacagt
     1081 agcagtatta tcatttggat gtatacctat tttatccatt tttaataaat ccttatcaaa
     1141 tacaactttt acaacagcct ctttatcatt taatatacca aaaggagtca cagctccttt
     1201 agtaagtttc aagtattttt gaagtctttc ttctgatgca aaactaagag gtttactatc
     1261 aatttgttga cgaatatctt ttaaatctgc ttttttgtct ttacctaata caactaaaaa
     1321 atgttctttt cctttagcat cacgaagaaa taggtttttt acaacaaatt ccatatgtaa
     1381 tgtttttaat tcttccattt cttcaattgt ataaactggt ggatgattta cagcctcata
     1441 aggtatgctt aactcatcta attttttata tacttcctgt tgttgattgt tcatgatttc
     1501 ctcccattag aaatttattt tatatataaa taatattatc acagaaaact ttaaaagaaa
     1561 acgttttata taatatagac aatagattat atttttataa agaagcaaga atactcaata
     1621 aaacttaaag aaattaaaca atttctaaaa ataccttgaa aatagaacct tatttgccat
     1681 ttataaatta tataaaaagg gattatataa agtcatatat gaaaaaacta tattatacta
     1741 tcttttcttt ttggaataaa ctatacttta aatatagata tttaatttaa ataaatttta
     1801 cagtattaat agtaaattat cttagaaagg aatgtgcttt atatgtctac agacaaagtt
     1861 gttggaatca tagatgaaga aacggcagaa cttgcaggaa ttgaatatac tggtaaaatt
     1921 tatgcttctt ctggtgttat aaaacatata aagaaaaaac atagatgtca actgagtaag
     1981 gatattttta atgatataat agacactata aaagtggtat taaagtcacc tgaatatata
     2041 ggaagtcatc ctaaaaaacc tggaaaaagt gtggagttta taaaaaaaat tgataattat
     2101 attttagtag ctacagaatt agatacaaaa aaaggatatt tatatgtatc ttcaatgtat
     2161 gctataaaag aagcaaaatt agaaagtaga gtaaacaatg gaagagtaaa acaatatgat
     2221 ggttaacata atattattaa ttttccccaa aatattgaaa aatattatta tataagttat
     2281 aatattaata tacacgtaga tatttattag ttagatttga ggttggaaaa ggcacccaat
     2341 acgctactat agagtagtta atagagatgc aggatacgcc gccctgccaa atctaattaa
     2401 attaaaaaga gaggattaat tcctctcttt ttgttttttt gagatactaa tttaatctat
     2461 atagaaagat tgaaggtcat ttatactaaa agttttcagt tatatacatt aaactaatta
     2521 aataagatat ttataatcta tatatgatac atattatata ttaataaaag aaaaatatat
     2581 agaaaatcct tataaaaatt taaattgtaa tgaatagtaa gtaattaaaa agaattttta
     2641 ttgattttta aaaatttagt tagaaactat atcattatgg taaagaatgt attataatgt
     2701 ttagaaatat tataataagg agcgattaaa catgaatcta tcgaagaaaa tagaagataa
     2761 aagaaaagct aaacagagag ctgaaaaagt taagaaagct aaaattgcta cagctggtgt
     2821 ggtattagga gctgttactg gtgcagtgag tggagtactt ttggctccta aatctggaaa
     2881 agaaacaaga gaggatataa aagatgcttc acaacaaatt gctgaaaaga taaatatgaa
     2941 aactgtagat gtaaaaggca aagtatctga gaagctagag gataaaaaag gaaactttat
     3001 agaatcaaag aaaaaaataa aaaaatatat agataacaaa aagagtgttg aaaattctag
     3061 tgaggaagat aaaataatag atatagttga accagttagt gttactgagg aataatatgg
     3121 acaatacaat aacgattaac ataagtataa tatatgtatt attagctata cttgcttgta
     3181 ttgctttagt ttatttaatt attgtacttg ttaaactaca taagatttta aataatgttg
     3241 gaactatgtt aaatgaaaat actcaaaata taaatacaac tttaaataat cttcctaatg
     3301 ttgtagaaac ttttggagaa gtaggagaaa atatgaaaga tgtaacagaa gtagttactg
     3361 aagttacagc agattttctt gttactaaag acagtgtcaa atcaaatatg gatgttatag
     3421 tcgatatatt gactataatt aaaaatattt ttctagataa aaagtaataa atttaatata
     3481 aaataagata taatttaaac gaccttaaaa taacatttat aaggtcgttt tttattagct
     3541 tgatttgaca ctatgtgttt ttaataattg gtaatattcc tctttatcat ttgaacatat
     3601 atatctatgc ttgttgtttc aattacatcc ataagaacat ctttacattc aagctcaaaa
     3661 tcttccattc cttctaaata ttcatcatct tcatcaaaaa agatataaga attatatagc
     3721 cattcttcaa atttttcttc aagtatatcc caatacgaac tccaaaataa tccacaacct
     3781 gatatataag atgctctact ttgatttata taaacctcat ttagaagctt ttgtaatcta
     3841 ctgctataaa gaaaatctgc tcttccactg cttgatatta attcatttaa atcttctatt
     3901 gacattaatt caactttacc acttttatca taaggagtat aattttcatc aacccattta
     3961 tgtggtttgt tttctctgca tactgttgct atcaactcta caacttttgt tcttatcatt
     4021 tttttatcct aacttattta ttttttataa aataatcatg attttataat ttatctattt
     4081 cttgtaatgc taatttaaca gtttcatatc tatcagcacc atttattact acatcacctt
     4141 ttacaagttt attagcttct ccacctacaa cataaacctt tttacctaaa ccttgcttat
     4201 aactagacgc ctcaattatt aaagaatctt tcaattgcca atgtaaaatc tgagcagcca
     4261 ctttatcagc atcacctgta taaacgatta cattttcata cactttattt tcccctcccc
     4321 cttgagaatt tgatatagat ttgtttagta taccttctgc tatcaattta gcaactttat
     4381 ctttattatt aacataataa tctgtatctg ctttactatc tacaaaacat acttctatta
     4441 atattgctgg tgcaatagta tgattaagcc aataaagacc tctaacatca gactttgcac
     4501 ccctatcttt gaatactgta gctaacctag tattcactct ttctgcatag gttttgccat
     4561 tattagtctt atatattgtt tctgtcccta ctggagttga agttgtagca tttgaattaa
     4621 aatgaatctg aacagccaaa tcagtttcct gtttatttgc tatagcacat tggtctgcta
     4681 aatgattact tgattcatca actcttcctg tataaacagt agctccacct attttaagcc
     4741 atttaacaat taagtcattc agtattcttg tttctttgct ttcgtttata tacccaacag
     4801 ctcctgtacc ttttccaatt aaagtgtgcc ctggtattat tactactttc aaactaacat
     4861 ctcctttaca attaaattta acttctattt aatatatgca taacctagaa ttttgtttct
     4921 ttttatatct tatactttta ttataaccca ttttgtaaaa atatagaata ttaagtaaat
     4981 aatgttaaaa ttataatttt gtatataaga cattacttga ttgcaataat tttatagcat
     5041 attcatctat tctatatcca gttctataat atatatgttt aattccagct tgaattatag
     5101 ccttagtaca atttaagcaa ggaaagtgag ttacataaat agagcaatct ttaactgaaa
     5161 taccttcttt tgcacaataa taaagggcat tttgttcagc gtgaatggtt ctaatacaat
     5221 gtccatctct catttcatga ccaacttcac tacaatgctt atctccagaa atactaccat
     5281 tatatccagt agacactatt ctgttttctg aattgacaat tatagctcct acataagctc
     5341 tatcacaagt tcctctttca gctactgttt cgcacagtct cataaaatat tcttgccatg
     5401 agcatctatt ttcaagtttt ttattcaaat catatacctc caaaatttat atctaatata
     5461 taagatttat accttaaaaa taatattaaa aacaaattta tacgataaat aaaatttatt
     5521 aatactgttt ttagtctaaa ttatttcatt ttaaattaaa gttacaagga atctaaaatt
     5581 atatatatct ttcttcgtaa tatgaatgtt acaatataaa aaactgtctc aataaaatag
     5641 agacagtttt acagtcaaag tttataatac aagttcatat ttttctttaa attcttttac
     5701 agcttcgggc catgcctttt gttctgtaat acctttttct ttagctattt taattgcaat
     5761 ctctcttatc attgcagcgc cttctaatat tcccattttt aatctccctt caaatatctt
     5821 tttgtataga atataaatag tgctaagatt tattacatgc tgcatataca atatctatac
     5881 aacacataac ttgtaaattc cttttatttt catacgtctt taaccgacat ataattatat
     5941 tcaataagtt ttaaaattat gaactcaatt ataattattt ttattttttt agaatctaaa
     6001 cactaaaaat tgtaggtaaa tttgtcttat gcctttataa atatttctac agttttaatt
     6061 actggacata tctattttta ttataaaaag cgcttaaaaa tcgagaatat ccgcgtaaaa
     6121 gctgtatata agcaaaacga ttaaaggaaa aagaagaaat ttacttgaat gttaaaatgc
     6181 gttgcttgaa gcaacgggca gttttgccta taatatcggt aacaactgaa agaaaggtgg
     6241 ttagccctaa tgttaagcga aaacattaaa acaatcagga agtcaaaagg actttcacaa
     6301 caagaacttg ctatcaaact gaatgtagtg cgacaaacaa tatctaaatg ggaacaagga
     6361 ctgtcggttc ccgattctga tatgttgatt tccatatcag aggtgcttga aacgcctgta
     6421 agcactttac ttggagaaac tattgttgag tcggaagttg atagcttaaa agtgatttct
     6481 gaaaaattag agattataaa cttgcaactt gcacaaagaa aaaacatgag aagaaaaatg
     6541 tttcattggt tatttatttt attatgtgca gtcatagtaa tagtttttgt ggtcttaggg
     6601 ggactaaata gtccttactt ggattggaat tataccgacc ctgaaaccgc tgtttttgga
     6661 gtagcttttc atgcttttga gtggctattt gtcagattag taccgattgt ccttatagga
     6721 gcaattattg gaatcttttt gacacgtaag aaaatatgaa gtaactctgc tttttataat
     6781 atagcaagcg ttccttaaaa gtgtcattct acatggcaat cgaggacaac tatgtaagga
     6841 agaacctttt tgatttccaa ctaagcgaag ttattgagga cgccagtagg cccaaaaccg
     6901 cattaagtaa agtacaggaa gcaagactgc tggacttata ctctcatgcg tcagatgcag
     6961 aaaatgcgag gtctgtagca tgatttacaa cgatttgtac aacgactacg agtagaaata
     7021 tatggagata agtattatct acgaaatcag aaaagaaatg acttgataat ttactcatgt
     7081 tataattatt gatgtgaaat taataaatcg aaattccttt tttagattta acaatactga
     7141 gtaaggaaaa agtgaatgac ttttgcaagg aggtgttaat atcaggcaaa taaagtcttt
     7201 tgtttttcca gttatatcct taatattctt agatcaaatt agcaaagttc ttataggatt
     7261 attcttaatg gactttgaaa ttgatataat tgggaaattt ttaagattca atcctgttca
     7321 aaatacaaaa ttaagtctct gctatctaac aaacggagca caaaaatgaa aattaaatat
     7381 taaatataaa gcacagttaa ttttgattaa aaagttgact gtcttttctt ttttataaat
     7441 ttaagcatgg gtaataaaga aatgactgca tttaagcatt atgtatataa ttggaggcaa
     7501 taaaaagcca gcaaagccca taaaatagag ctgtgctagc aacatgatga gttataagaa
     7561 gataatcaaa agcttacgct ttattttaat ttaaaaaaat gattactgta aagaaatcat
     7621 tttttattat aatcactgat acatcaaata ttctaaataa aatttttatt tatctggaat
     7681 tgtatatttt ttctataata ttaactacag cgtacattgc ccaagcacaa atagccaata
     7741 caaatactcc catcattact aaatctaatt taaatacttg agaaccatat actatcaagt
     7801 agcctattcc atatcttgaa actaaaaatt ctccaactat tacacctacc catgccatac
     7861 caatattaat tttagttaaa tttattaaat ttcctgtgtt tgcaggtaat atgagtttaa
     7921 ataatatttg agatttagta gctccaaagc tttttaacat cttaattttt tcttcatcta
     7981 tattcataaa ataattataa gctgaaagta tagtaacaac aacagaaatc gttacagctg
     8041 ttacgacgat tccttctatt ccagcaccta cccaaactat aattatagga gctaaagcag
     8101 tcttaggaag agcatttaaa actaccaaaa atgggtctaa tatttttgat aatttttctg
     8161 accaccaaag agcaattgct accaaaatac ctaatacagt acctataaca agaccaagaa
     8221 cagtttcata tacagatatt ccaatatgtt taaataattg accactaaat gcatattgaa
     8281 taaataaatt gtaaatatca cttggtttgc taaatagaaa tgtatttata acatttaagt
     8341 cagctaaaag ttcccagatt acaataaaac caattaaaat taatagctgg tagaataata
     8401 catttctttt ttctcttttt acacctttaa catagtttat atatccttca gatcttttat
     8461 taagcctcat gtgtatctag ctccttccat aatatgtcga aatacttttg aaaatcagga
     8521 actttacgtg ttttgagagg cgtttttgtt ccatccatgt taagttcaat atcgtaaata
     8581 ttttttatgg tagctggtct tttagatagt attgcaacct tatctgatat gcttattgct
     8641 tctgctatag ctaaaaaagt gcaactaatt gcttaaagaa acagaattat ccacttcagc
     8701 acccatataa acaatattaa gatttcctaa actgctatcc caaatgatac tattaataat
     8761 agtagataat aacaatctct tatcatctaa actggcatta tcaacttcat cattaaatct
     8821 cttcaaatta ctaacaataa tatcaatatt aatactgtca ctactaatat tgatattact
     8881 agattccaaa atattaatct tatcctttaa aatactaatc tcattattta ggctctcaat
     8941 ctgggaaact atataatcag aagcagtact accagtattt tttgcaagct ctataactaa
     9001 atttttaata agtctttcct tatctaaaat ttgattttta agtaaattag tctcagattt
     9061 aaaaatatta aaaacattat cagtcttaat gttattacta ttgaatgcac ttataataga
     9121 ctcttcttta caattcttaa ttttatctat aactaaactt tcaactaatg gaccattaag
     9181 atttttacag ttgcaactat cagacccaag tgttttttta gtaccacaaa tataataata
     9241 ctttaaatcc ttgtcagttt ttttgccttt ataggtaatt ctcatattag aaccacattt
     9301 accacatttt ataagaccag ataaaagagc agtttttcca gttgatatcc ttggagtttt
     9361 atcacgattt ttatctaatt gattttgtac ttcaagccat atttcaggtt ctatgactcc
     9421 atggtgtttt gcaattgctg ctattgagtt agatgtattt ttagcatagg taaggattcc
     9481 acattttcta tctggagtac ctacaacatc cataccacta tttttaaggt aatctaaaat
     9541 ttcatcatta gcttttacat atgctggatt tctaagtatt ctagataaag aactagggtc
     9601 aaatttacca ccacgaggac cccttattcc attattatag acatatttat ataacttaga
     9661 taaggaccta agttctaaat atttattgta tattagcttt acaatctcca tttgttcatc
     9721 atttacttga agcttataca tacttctttc ttttagattt tcatctaaga atgtgatttt
     9781 ttcactgttg aatccatgag gtgtattgcc tccaagccat ctaccactac gggctaattc
     9841 atacatgttg tcttttatac gttctgctat tgtttctctt tctaattgag caaatacact
     9901 tgatataaac atcatagctc ttcccattgg agttgaagta tcaaattgtt ctttgataga
     9961 tataaatgat atgtttagtt catttaattt ttctattaaa gtagagaagt cagatacatt
    10021 tctcgatatc ctatctaatc tataacaaac taggtaacta aatttcttat ctttagcatc
    10081 ctgcatcatt ttcttaaatt gtggtctatc tatattgcta ccactaaatc cctcatcttc
    10141 atatatcaaa aaatctttta caccaataga ctttgcataa tcaatacata attgtatttg
    10201 attttctata gaatcacctt ttccagtaaa tcttgatttt cttgaataaa ttgcagcttt
    10261 caaaattaaa acctcctata aattaggata tatatttgaa attatttata tacaaccttt
    10321 taagtaaact tattagaata aatttattaa ttaggtaaat
//
LOCUS       DAELXX010000125        10359 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.126, whole genome shotgun sequence.
ACCESSION   DAELXX010000125 DAELXX010000000
VERSION     DAELXX010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10359
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.126"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            145..924
                     /locus_tag="KPH29_003147"
     CDS             145..924
                     /locus_tag="KPH29_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HBG1468719.1"
                     /translation="MNYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKVFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVVLGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDVVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
     gene            complement(1234..2055)
                     /locus_tag="KPH29_003148"
     CDS             complement(1234..2055)
                     /locus_tag="KPH29_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallohydrolase"
                     /protein_id="HBG1468720.1"
                     /translation="MKLKIVGSGGMSIIPSSFCKCKICEEARQKGGRYERLGPSLYID
                     DIKMLIDTPEDITVACNRQKISEVKHISISHHDPDHVKGIRIVEKIGCDFITGENNPI
                     GFYALPEVIEDINRTNLDCLKYYGDVINCISINETSHVKINNFDIDLINNNPDRNITF
                     YVIKENEKKVIYACCNPKPFTHSDMYFDADVLIISLVSDDGILNDGTKLEDTPFKDEI
                     FTLDEIVEIKNYYRIKKIIITHIDEMWGKSYGYYSELEKKLDNIYFAYDGMEIIV"
     gene            complement(2780..3598)
                     /locus_tag="KPH29_003149"
     CDS             complement(2780..3598)
                     /locus_tag="KPH29_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG1468721.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNNTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQAFLETKDQNV
                     QIEPPKYSKAVFYDTGYKEDSVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
     gene            3766..4347
                     /locus_tag="KPH29_003150"
     CDS             3766..4347
                     /locus_tag="KPH29_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG1468722.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            4344..4538
                     /locus_tag="KPH29_003151"
     CDS             4344..4538
                     /locus_tag="KPH29_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468723.1"
                     /translation="MKIITIGSSLITVLLFLSTMVCGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYIKK"
     gene            complement(4702..6444)
                     /locus_tag="KPH29_003152"
     CDS             complement(4702..6444)
                     /locus_tag="KPH29_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HBG1468724.1"
                     /translation="MVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGKDTFNYLKNIG
                     KLNSKEVEDLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQDGNLEEISSTLYD
                     YFGIDNPLELKNAS"
     gene            6873..8249
                     /locus_tag="KPH29_003153"
     CDS             6873..8249
                     /locus_tag="KPH29_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBG1468725.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            8560..9231
                     /locus_tag="KPH29_003154"
     CDS             8560..9231
                     /locus_tag="KPH29_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBG1468726.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            9422..10219
                     /locus_tag="KPH29_003155"
     CDS             9422..10219
                     /locus_tag="KPH29_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBG1468727.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDMNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
BASE COUNT         3839 a         1243 c         1579 g         3698 t
ORIGIN      
        1 tttatgctat ataacaatta ttgaaaatga tataaatata atatctcaaa tgatatgaat
       61 ataacatctg aatagagggt gttttatttg taaaaaataa gtttacagtt tgagcgtata
      121 aacttatggt aaaggagaat aattatgaat tataagaaga ttgcaattat aacgggtgct
      181 aatagtggtt ttggtaaaga gtttctaaag ttattaataa atgaagaaga aataacagaa
      241 atatgggcag ttgcaagaaa caaggcgaga ttaaatcaat tagtagatga atttggaagt
      301 aaggtaaaag ttttttcaaa agatttatca aaaatagaag aagtaaaaga aattggtaca
      361 tttttaagta aagaaaatgt atgtataaaa taccttataa acaatgctgg ttttgcaaaa
      421 ttctgttcat acaatgattt aagtatagat gaatctatta atatgattga tttaaatatt
      481 agtgctgttg tagtattggg acttatatgt attccatata tgcaaaaggg aagccatatt
      541 atgaatattt cttcacaagc atcattccag ccattgccat atcaaaatat ttatagttca
      601 actaaagcgt ttataagaaa ttatacaaga gcattaaatg ttgaacttaa gagtaaaggg
      661 ataaatgcaa ttgctgtatg tccaggttgg atgagtacaa atctatttaa gagaggtata
      721 gttgtagctg aaaaagggac aaaaaatttc ccaggcatag tgtctccaga cgttgttgcc
      781 aaaaaagcat tgaaggatgc aaagaaaaat aaggatattt ctgtgtatgg tataaataca
      841 aaatccagtc atttattagc aaaactattg ccacaaaaaa tgatgatgaa ggtttggtta
      901 atgcaacaac atattaagga ataagagttt cggtaattaa gtatcagttg gagattcaat
      961 ctattatata ttataagaag ttttgttgag caaataataa ttgtgacaaa acggcttatc
     1021 tagtgccgat aagccgtcta taatgtataa aaaatatatt tttgatttta ctttatttag
     1081 atattgaagt attaaaatat gtaggacttg aaattttata aagataaata ctatgatatt
     1141 ttgtaaaaac tgagatatgt tgatattaca tagtttaact atactgttaa attgaagttt
     1201 tcttattttg cattatgctt attacttaat gatttagact ataatttcca taccatcata
     1261 tgcaaaataa atgttatcaa gtttcttttc taattcactg taataaccat atgattttcc
     1321 ccacatttcg tcaatatgtg taatgataat ctttttaatt ctataatagt ttttaatttc
     1381 aacaatctca tctaaagtaa atatttcatc tttaaatggt gtgtcttcaa gttttgtacc
     1441 atcatttaat ataccatcat cagaaactag acttattatt aaaacatcag catcaaagta
     1501 catatcacta tgagtaaatg gcttgggatt acagcaggca tatatgactt ttttttcatt
     1561 ctctttgata acatagaagg ttatattcct gtctgggtta ttgttaatca aatcaatatc
     1621 aaaattattt atctttacat gcgaagtttc attaatagaa atgcaattta taacatcacc
     1681 ataatattta agacaatcga gattcgttcg gtttatatct tcaattactt caggaagagc
     1741 ataaaagcca attggattat tttctcctgt gataaagtca catcctattt tttcgacaat
     1801 tctaataccc ttaacatggt ctgggtcatg gtgagaaatt gaaatatgtt ttacttccga
     1861 tattttttgt ctgttacaag ctactgtaat atcttcaggt gtgtctatca gcatttttat
     1921 atcatcaata tataaacttg gtcctaatcg ttcatatctt ccgccttttt gcctggcttc
     1981 ttcacatatt ttgcatttac aaaatgaact tggaataatt gacataccgc cactaccaac
     2041 aatttttaat ttcaaagtta ttacaccacc ttctaaaaat attagattat ataaataatc
     2101 tacaatcaca actgattaac aaaattatga gttaataaat tatatcacat gtaaatatga
     2161 aagaatagta tgaaattttt ataagattat attaaatatt tctcaatgaa attgactttg
     2221 tatatgagcc gaatgacttt attcaatata gataagacat ttttcttgta ataagatatg
     2281 attattctat aaggttcatg taattttgat ataaaatatt gattaataac atatatgtta
     2341 ttggtgaaca tatttctata gaatggaata tataattttt tatatttatc attgtattat
     2401 tatacaaaga aagttaaaaa gagtagagta caaaggaatt atttgtactt tactctttgt
     2461 tttgttaaaa actaatttat attagtgatt caaatattta actagagtat aagggttgta
     2521 tataatctta taagtaattt atctgacaaa ttaagataat atcctaatca aattctagtc
     2581 attcgaacat aacatcacaa gtgatttatc tgacaaatta agataatatc ctaattaaat
     2641 tgtagatatt tatacataac actaaaagta atttgtttaa caaattaaga taatatccta
     2701 attaaattgt aggtatttat acataatact aaaagtaatt tgtttaacaa attaaaatat
     2761 tatccaatta atataaaaat tacttctttt taactgggaa gcatacctct gttacccaat
     2821 tatcaggatt actatcgttg ccaggactta catgataaat attaaacata ggaccatcca
     2881 catcatagtt attatctgat atccaattac caatagcctc acaagcgtct gcaacttgat
     2941 tgaaatttcc attaacaatt gcagttgcag cagtcacact tggaacagtc ttaaatttaa
     3001 catgttcagt atctttatac tttccagata cagcaacttg tacttcaaca tctacagaat
     3061 cttctttata tccagtatca taaaagactg ctttagaata ctttgggggt tcaatttgaa
     3121 cattctggtc ttttgtctct aaaaatgctt gatgccataa cattccttcg tcttgataag
     3181 ttggtataac ccttctaagt gtcatcatat attttggtgc aaaattcttt atagttacat
     3241 cataattttt cataatatca tttcctccaa ttcttttaat agtggtttct atttttaata
     3301 gttttttctg tgtatcttct aattgttctt ttacttgaga atggtggata tttagatatt
     3361 ttataagact ttctttatcg ttatattctg tcaaaatttc ttttatagaa tacaagccaa
     3421 agcccatatc ttttagtgca tggattctat ttgtagtaga aagttggtct gcactatagt
     3481 atctatatcc attagtgtta ttcgtatgag aaggagttaa aagtcctatt tcatcataat
     3541 gacgtaacat acgaatacta attttactta gctttgaaaa atcgcctatc ttaaacatgt
     3601 ttttgtctcc tttataaagg tacttttagc ttaatattaa tataaaccat atcataatgt
     3661 tagagtcaac taaaattata attaaattat attgacatat gatgaaaaaa atattatact
     3721 gaacatgttc agaacctaaa atgttaatta ttttggaggt ctattatgaa aagtaatgat
     3781 aaagatgcta gagaacgtat aattgaagtt acattgaatc ttttaaatga agtagatgat
     3841 attgaggaga ttacagttag aaaaattgct gagcgtgcaa atgttggagt tggtttaata
     3901 aattatcatt ttaaaacaaa ggataatttg ttgagtactg ccataggaga tgtgatgtca
     3961 aatataatag cagagctata tgacgatagt gtatatactc taagacctat tgaagatttg
     4021 aaaaatttat taaaaaaatt gtgtgatact ggattacatt atgaaaaagt gcttcctttt
     4081 gtgttgaatc aatgtataac caatggagat atgcaagcag aacttgatat tgtccctatg
     4141 cttcgtaaaa tttttggaaa taaaaaagat gaaatgagtc taagaatcat tgccctgcaa
     4201 attattttac caatacagat ttctgcttta tctacagaat catttcagtt atacagtggt
     4261 ataaatataa agaataaata tgagcgagat aaattcattg atattttaat agaaaatatt
     4321 attggagagg atgttgatgt aagatgaaaa ttataactat tggttcaagt cttatcactg
     4381 ttttactttt tctttcaact atggtttgtg ggttctggat taagaataat aaggtaacag
     4441 atgcaagttc tattaagttc catatgaata gtgctatttt tacagggata tttttattga
     4501 tttccacaat ccttttaatt atatatataa aaaaataata atattattaa taaataaagt
     4561 atcagaaatt ataaagacaa gagtcttaaa aatttctgat actttaccac ataattttat
     4621 taaacattaa ttgtgtgtga aaaaatatca cttatttcaa tctaatacta aatcttaaga
     4681 gtgagtataa agtattagaa attaagaagc attttttagt tcaagtggat tatctatacc
     4741 aaaataatca tataaagtag agcttatttc ctctaaattt ccatcttgga aaggtaattg
     4801 taaatttacc ttttctacca catctttaaa tccagcatca gtattttgat ttaaaaaatc
     4861 catatataca tcaacagctt ttctgtaatc ttcttgtgat aattgccata tctgaagtga
     4921 tacatcagat gcaagaccat aactcaagta ataaaaagga acttggaagt tatgagatac
     4981 aagtatccaa aaaggagcat ttttttctct aagtatatta ttggaaactc catattcttc
     5041 ctctaaatca aaaaacaatt tattgatatc atttagactc atatatggat tcttgtatat
     5101 aatttcttga aattcatcat aaagacaagc atcaataata gtatttaaaa caatcgataa
     5161 gtgttctttt tttatagctt cagattgttt accaaaaaaa tcatcaaaat atttataaaa
     5221 tagaatttct aaagatgttg aatgaacttc acatacatct atacttggtt gcatcttatt
     5281 atttaagtta atactgttgt agtaattata aaaatgacca aactcatgag caaaactagt
     5341 aacacctaaa aaagaattgt cccatgtatt aaacaagaaa ggtgctttat atttatttat
     5401 ataagtagta tatcctccag gcgatttgtt ttttccagaa gaataatcgt acaagtcata
     5461 ttttttcata tagtcgaagc tctcttttag ttttggagat atatcttcaa gtactttatc
     5521 aaacttccta aatgagcgat ttttatatac tttaatataa atagaagaat cagatggttt
     5581 agatgaaatc tctctatata atggaactat gtaatctttt acatcttgtc taaatttttt
     5641 agcttcttct tgagaatagt ctttgtttag gttcatatag gcataatcta tataattctt
     5701 aaatccttgc ttacttgcta tttcagttct tgtctgtata agttctaaga atatgttacc
     5761 aaatatagga ttgtattttt ttaagtaatc tgaataaatt ttcacatatt cttcagggct
     5821 aagatttggt cttgacaagg cttcttcaaa gtctacctct ataccatcta ttgatactgt
     5881 agatttagct aataagtctt catattttac aactaggtct tgctcttttt taagtaaatc
     5941 ttcaacttct ttagaattaa gttttccaat gttctttaga taattaaatg tatcctttcc
     6001 aagttctttt tctaattctt tagagaattt agaagttaca aatctatctt gaaagtcttt
     6061 gtatgacatg tcaacatcta ctgatttact cataagatac ttatattcct tagagtaaaa
     6121 ttcgtctgat gaatctatgt tatttctaat ttttgctata gtaagtgtac tgtaaaaatc
     6181 ctgataaatt ttatcaactt cattaaataa atttaattgt tccttagctg ttttagctgt
     6241 taaaagtttg tcgttacact tgttaatgtt ttcgcaaatt gactgtatat taggacgctt
     6301 atattccatt ttttcaaaag gaatagttcc tctaacagaa tctttttttg tatcattagt
     6361 ataatttctt atgtatgatg caaatataat aaaaactgca attaaaatta gaaaaacaaa
     6421 tattttcttc ttggatttaa ccataaaaat cctccaatca tggatatagt tattaattat
     6481 tttactatat taaatataaa aaatgcatgt taagaaaatt taacataaag atatagataa
     6541 gattataaat aatataaaaa atttaacttc aaaaaagtat atgaaattta tactggtaaa
     6601 atgataaata aattttaagt acaaaatagt aaaagctatg atagttttag acccattata
     6661 tgtaaaaaat atagatgttt ttggtgcaat ttaaattaaa agatatatat aaaaatttaa
     6721 aatattccaa acagaaatta taaaaattac tatcattgtc tttatgcaat atttatacta
     6781 taatagaata ataatttaga ggaaagctct aaaaataaac aaattaaggg tcttaacagt
     6841 agtgatttta tgttaaggag gtataaagaa agttgaaaac aatagttaga agacttattc
     6901 tctacgcaga tggaatgcgt cctactcaaa taatggtatc tgggtttgct gcgattattg
     6961 taatcggagc attgttgtta acactcccga tagcgtcgca gagtggagag agtataggct
     7021 tattaaatgc tctatttacg gctacttcag cggtttgtgt tactggtcta gttatggtag
     7081 atacagcaac ttattggagt ttatttggac agatagttat aataacattg atacaaattg
     7141 gtggtcttgg atttatgacc gtagccacaa tgttttcact tatggcacga aaaaaaatac
     7201 aactaagaga aagattatta atacaagagt cattaaatca agctgattta tcgggacttg
     7261 taagacttac aagatttgta ttaataataa ccataacaat agagggaata ggtgcactgg
     7321 ttttatcaac tgtctttatt ccacaatttg gtttgtctaa agggatatgg tatagtgtat
     7381 ttcacgcaat atcagcgttt tgtaatgccg gatttgactt gatgggcagt gtaagtggcc
     7441 catttacatc gttaaattct tatgtaaaca actttacagt atcaatgact gtttgcgcac
     7501 ttatagtgct tggagggcta ggattccctg ttgtacttga tattgtaaga aaaagaagat
     7561 tttcaaaatt aaatgtgcat tctaaggttg tattgttttc aactgctaca cttatttttg
     7621 tgggtgcatt atttattttt cttatagaat ttaatcaaaa agctactatg gcggacttgc
     7681 ctttaaaggg aaaagtacta tcagcaatat ttcaatcagt aacagcaaga actgctggtt
     7741 ttaatacact tgatttagct actttacgag aaagtagtgt atttgtaatg ataattttga
     7801 tgtttattgg agcatcacct gcatcaactg gtgggggtat aaaaacaaca acactagctg
     7861 ttttaatcat tacagttaga agttttctat caggaaaatc agatatagaa gcttttgaaa
     7921 gaagattagc tccatcaact ataaaaaaat ctcttggtat atttgtaatc agtatatcag
     7981 cagttatttt tggaacattg attatttcaa taactcagcc taattttaca ttagttcaat
     8041 cagcttttga ggttacatca gcacttgcta cagttggttc aagtttagct ggaactccaa
     8101 atttaaatgc tttaggtaag attataataa taatctttat gtttatgggt agagttggtt
     8161 ctcttacttt atttatggct atactttctg gtggtagaag aaagagtcaa ccaatcagat
     8221 atgcagaagg taaaattatg gttggttaag ttaccaagga gtaatataaa tttataatgg
     8281 atttagagta gcactgtaaa tctgttgata aaataaatga gcctagatgt aattatatga
     8341 agaaataaaa tcaactaata aacaaaatta attaatgaaa taaaaaaaca gggaaataaa
     8401 atacaaataa aaaggtaaat caaaattaaa ttaatctgtg tatataaaat tatatattgt
     8461 gtataagttt tgtatgtgta aagtatataa gttttgtatg tgtaaacaat taatttaaga
     8521 gatattatat aaattgtgta ttagggagga agtttcgaga tgaagcaata tatagtcatt
     8581 gggtgtggga gatttggaag ttcagttgcg tctactatgc atcttttagg acatcaagta
     8641 atggcaatag acaaaaatga agattcagtt caaagtatat ctgacaaggt aacccattca
     8701 cttatagtgg atgttactga tgagcaagcg ttaaggtcat taggtttagg taactttgat
     8761 gtagcagtag ttgcaatagg ttctgatata agggcatcta taatggcgac tcttatagcc
     8821 aaagaaatgg gtgtagagtt gataatatgt aaggcaaagg atgaattaca agctaaagtg
     8881 ctttataaaa ttggcgcaga tagagttgta tttccagaaa gagatatggg agtaagagtt
     8941 gcacacaatt tagtttcgga taatatatta gaccatattg aacttgaccc agagtattca
     9001 attgttgaaa tcgtaactcc aaatagttgg gttggcaaga cacttataga gcttgaatta
     9061 agagctagat atgagataac tgtacttgct ataaaaacag gtaaaaatat aaatgttaca
     9121 ccttctccag atgaggaact tacagccgga agtatcctag ttataatcgg tcaaaatact
     9181 agtataacag cgataacatc tggaaataag gggataatta gaagaagata atttactatt
     9241 taatatatat ttaattgcaa tgaaagtaaa gagtatcata taattatgaa tagttatatg
     9301 atactatttt tatttaatcg aaggtagtat ttatttgtaa gattagataa agagaagtta
     9361 aattaaaagt aaggaggctg tgctaatata aaaatttata agttattagc atttagataa
     9421 aatgattaca aatataaaca gtaaggataa tgaaaagtta aagtatacaa gagcactatt
     9481 aaaatcaaag aataggaata aagagtcaaa gttcataata gaaggataca gaatagtaat
     9541 gcttgcactt gaatgtatgg caaaccttga ttatgtattt atcaatgaag aatttgaaaa
     9601 taagaaagaa catgtaaaac tattagaaga tttggataaa aaaaacacaa agatatacaa
     9661 gactactaat aaaaacttta aagaattagt ggatacagaa aatactcaag gaataatagg
     9721 tgtagtttca tttaagaaaa aaaaattaag tgaaagtata aataaaaaag ataaatttgt
     9781 attgatttta gatagaatac aagacccagg aaatatgggg actataataa ggactgctga
     9841 ttctgctgga gtagatgcca taatagcact aaagggatgt gtcgatatat acaacccaaa
     9901 agtaattagg tctactatgg gttctatttt tgatatgaat ataattgatg cttcacaaga
     9961 tgaaactgtg gacatgctta aatcattgga ttttaatata gtttcaagtt acttaaatac
    10021 agaaaatttt tatgacaaaa tagattatgg ttcaaaagta gcattggtga taggaaacga
    10081 agcaaatgga ataaatgaag aacttgtatc aaagtctgat attttggtta agatacctat
    10141 atatggtaaa gccgagtcgt taaatgctgc gataagctct gctatactga tgtatgaaat
    10201 aaaaaaatac ttaatttaat gtattgtaat aaatataatg tcatgttata atcttgaatt
    10261 aaatagtaga gtatcaaaaa tagtcaatat atattaaaaa aataattaaa ttaattatat
    10321 attagatgta tatcatataa aaaatgattg agttgatta
//
LOCUS       DAELXX010000126        10278 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.127, whole genome shotgun sequence.
ACCESSION   DAELXX010000126 DAELXX010000000
VERSION     DAELXX010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10278
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.127"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            205..2394
                     /locus_tag="KPH29_003156"
     CDS             205..2394
                     /locus_tag="KPH29_003156"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HBG1468728.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYDISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKRFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEEFIDIKTDLEKVTKANISIRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEEDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
     gene            2462..3229
                     /locus_tag="KPH29_003157"
     CDS             2462..3229
                     /locus_tag="KPH29_003157"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBG1468729.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            3287..4303
                     /locus_tag="KPH29_003158"
     CDS             3287..4303
                     /locus_tag="KPH29_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBG1468730.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCANSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVYAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            4369..4617
                     /locus_tag="KPH29_003159"
     CDS             4369..4617
                     /locus_tag="KPH29_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468731.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELGVLLEEKKELEEE"
     gene            complement(4794..5291)
                     /locus_tag="KPH29_003160"
     CDS             complement(4794..5291)
                     /locus_tag="KPH29_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBG1468732.1"
                     /translation="MYKIGESVMYPKEGACYISGLVTKDINHHIQKYYELTVIYNSNL
                     KISIPVLNADKIGVRPIMDENEVENFIQSLDKVDCLWVFDRKKRLKLYHDKFHSGDVF
                     EIVKLIKMLMIQDNSKQLCSTDKDFLNKAQRFALSELAAAQCKSYTIVLEEMKNHILN
                     SKNNN"
     gene            complement(5532..6596)
                     /locus_tag="KPH29_003161"
     CDS             complement(5532..6596)
                     /locus_tag="KPH29_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1468733.1"
                     /translation="MIRLTIANIKRYIKNHALLVNMVLFPVILMFYLNFFTGNSENQS
                     MYFSPVAIVSNSSGKYEHKLIDSSKLKENSFGLNEQDKAMDLLKNNKVSAVFILDKNF
                     SSNIDNLKRPEVKCFKTENGGGSLWAESQIESFITNSLKLKIDRNIDDKLTKTNIIDN
                     SSGKNKNSFLIVFLICYFMYINAAHLASDLFSLKKSNTLKRLVSTKHNDIKIIFSIFL
                     GLFFTQAIVYTLTLVCFTSISNFKLTLNTLMIVLANSFVATGFVFWIARVFKNEGSIS
                     LMSTFYSLIGLAISISNMIPSLDKLSFMANLSKLTPFYWTIDAINNNGNILINIIVLI
                     LIGMVFVTAGSLNLRDFARN"
     gene            complement(6599..7696)
                     /locus_tag="KPH29_003162"
     CDS             complement(6599..7696)
                     /locus_tag="KPH29_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1468734.1"
                     /translation="MKLYISVKTMLKVLKSSFMLNLIYFLALPLVLAGFLGMVTESIF
                     QNPIKTDLTSIVIYDKDNTKLSSNLTGYLKNDLSSILTVQKDDSKADLKLTIPKGYEN
                     NLLNEKSNNLSIEKLKSNDNIAILLQDILDTYHEKLYLNNSQKLSSEDFSKLFNKNSI
                     DTSIIKNNVKQNSYEYFALVSLGFFAIIFIMNSILSNYVSESKGLSKRLYSIPVTRVQ
                     FLIYDFIGFWIYSFIFLSLYVLFFRTLGVTFKGNFVILALLCALASYFMTSISTFITT
                     FFSKKYGTVIVYALLFLQTVFGGIFSIVNDVFTKFTSLSPTYLIGTLFSNYETFKTID
                     SIESLIFTCLIASTVLIALSIVKEKYKWREV"
     gene            complement(7710..8645)
                     /locus_tag="KPH29_003163"
     CDS             complement(7710..8645)
                     /locus_tag="KPH29_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1468735.1"
                     /translation="MDIVRVNNITKRFNDKLALDNISFSVKEGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGDIQINGLDLSKDCIKAKSIIGLVPQELALIETLTPFDNLEYFGT
                     YYGLKGKLLQERITEALEVTGLTDVKKKKIKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKNISKKQKTTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGD
                     NDYLKSLVSTNTKLIMEIKNINAQLIFDLKNTKGVVSILENNSLLELGVDKKLQLTDV
                     LSVVDKNNSKITRISYEEPSLEDVFLNLTGKNLRD"
     gene            complement(8833..9477)
                     /locus_tag="KPH29_003164"
     CDS             complement(8833..9477)
                     /locus_tag="KPH29_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1468736.1"
                     /translation="MNKIKVLIVDDEKLIRKGLNIILSSYDDLEIVGDASNGYEALEF
                     CKTNDVDIVLMDIRMKVCDGVLGTRLIKEYDSSIALLILTTFNDDEYIEDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMYPQKTIEQKHISDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILGKLELRDRTQLVIFAFKNKIVI"
     gene            complement(9470..>10278)
                     /locus_tag="KPH29_003165"
     CDS             complement(9470..>10278)
                     /locus_tag="KPH29_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG1468737.1"
                     /translation="KNLYLALESSSTLLIITLFSVYLENENLSKLYNQSLYDKLRVSK
                     DELKKANADLEIYANSIEELAILKERNRISREIHDSVGHSLSTTIIQLNAIEKLLKDK
                     PLISDLVHELREFVTESFQDVRRAISELKPIEYENYQNLLKIRELVNSFIKLTNINVR
                     LTISKNTWNLSTNQSIALYRLVQESLSNASRHGKATEIRIFITFNPSNLIITISDNGI
                     GCNNIKRGNGLSSISERIYELNGKVEFDNSNTGFTIRASFPKFSGGDFFE"
BASE COUNT         3897 a         1215 c         1632 g         3534 t
ORIGIN      
        1 cttttttata ttatttttga gttttttagg aagatatttt acaagttaag aaaactataa
       61 ggagtgttat attgaatata aaatagtata atatatatta tgttagataa ttaatatgaa
      121 aatatacaaa tatataaaaa agtttgttta gagttatagt taatgtgtgt attaatttaa
      181 tactaattta ttggaggttt aaaaatgaaa tacataataa aaagagatgg tacaaaagaa
      241 ctttttagta aaagtaagat tgaagatgct gtctataaag ctagtataaa tagtgaaggt
      301 ggagtagata gagagttggc atatgatata tcaagaaaaa tagctaatga ctataattct
      361 atggaaaaat gcttatccgt agaagaaata caagatatag ttgaagtact acttatggaa
      421 tcaaatagaa aagatatagc aaaacactac atattatata gagaaagacg ctctgaaata
      481 aggactaaag cttgggagtt tgatgaatta caaaaatcaa tatggaagaa caagtataga
      541 cataatggtg agagttttga tgaatggata aagagaattt caaataatag ttcaagaata
      601 tcaaaactaa taagacaaaa aagatttcta tttgctggaa gaattttagc aaatagagga
      661 ttacagaaag aaggaaaaaa agtaacatat tctaattgtt atgtaataga accaccaaaa
      721 gatagcatag aagatatatt tgatactgca aaaaaacttg ctagaacttt tagttatggt
      781 ggaggagttg gaattgatat ttcaaagcta agaccaaaag gagcgaaagt taacaatgct
      841 gcaaaacata cgactggagc agtctctttt atggaacttt acagtatgac aactggctta
      901 attggtcaaa gagggagaag aggagctctt atgatttcta tggatgtaaa tcatccggag
      961 atagaagagt ttatagatat aaaaacagat ttggagaagg ttactaaagc aaatatttcc
     1021 ataagggtaa cagatgaatt tatgcaagct gtaagggaaa aaaagttata taattgtact
     1081 tttgaagttg aaactactgg agaaaaaata gttaagatag taaatgctta tgatttattt
     1141 aataaatttg tgaaaaataa ctgggattat gcagaaccag gtatattgtt ttgggataaa
     1201 ataaaaacat ataattttat gagtgaagat aagaactttt gcttttcagg agtaaatcct
     1261 tgtgcagaag aaccacttcc aagtggagga agttgtctac ttggctcaat aaacttgtca
     1321 gagtttataa tacaaccatt tggtgaacat gctacttttg atattaataa attaaaatct
     1381 tgcgttagag aagctgtagt agctttaaat gatgtcttag atgaggggtt gaacttgcat
     1441 ccattacaag aacaaagaga tagtgtacat aagtatagac agataggttt aggtgtaatg
     1501 ggtatagctg atatgcttat aaaaatggga ataagatatg gttctgatga atctttagaa
     1561 ctatgtgatt taattggtaa gactatgtta aatgaagctg tgaagcaatc agcattaatt
     1621 gctaaagaat atggaacatt tagtgagtat aaatatgaaa atatttctaa atctaagttt
     1681 tttatagaaa atctaaatga agacactaaa gaaatggtta aaaagtatgg gcttagaaat
     1741 tcacaacttt taaccatacc tcctacaggt tctatatcta caatgatagg tatcagtggt
     1801 ggaatagaac ctatatttaa tctttcatat ataagaaaaa ctgaaagctt gcatgatgag
     1861 gatgtgtatt ataaagtata tactccaatt gttaaagagt atatggacaa gaaaggtatt
     1921 acagatgaag aatgtttacc agattttttt gttactgcta tgaatatagg atatgaagaa
     1981 agaataaaca tgcaaaaagt atggcaaaag tatattgatg cttctatatc atctacaata
     2041 aatatacctt ttgaaactac agttgaagat gttttcaata tatatattaa ggcatgggag
     2101 gctggtctta aaggagtaac aatatttaga gatggatgta aaaggagtgg aattttaata
     2161 aatgaaaaaa aggaagaaaa taataaagaa gataaaaaag aaaatagtaa aaaagaatat
     2221 tataaaaata ataaaaaaga aaaagataat aaagatgaaa aagccaaaga aataataaat
     2281 aaaaattttg aagaagatga aaaatttgtg tgccctgaat gtggaagtga aggaattgca
     2341 catacaggtg gatgtagtat atgtttaaaa tgtggctata gtggatgcaa ttaaactcta
     2401 attaaaaaat tttaatatat ttaaaaatta aatttttaaa aacgaaagat gagggaatgg
     2461 tatgcaggat aaaagtgtga gggagattaa agaaataata gagactttag aagtagaaaa
     2521 atatatggaa tatatagaac tgttaagagt tgatgaaaga aaatctgtac aaggtctagc
     2581 tataaaacta gcaaaaaagc tggataatat tagaaaagaa gaagaaagat tggaaactat
     2641 aaatatattt gagaacgaag gctatgataa aggttatttg tatattggag gaattgatga
     2701 ggcgggaaga gggccacttg ctggacctgt tgtagcttct gttgtagtat ttaaaaaaga
     2761 tacaaaaata gaaggtgtaa atgactctaa gaaactgagt gaggcaaaaa gagatgaact
     2821 ttttgaagta ataaaagaag aagcactaga ttatggaata ggtatagtaa ataatgaaga
     2881 aatagatgaa tttaatatat taaatgctac atatatggca atgaaaaagg ctataaattg
     2941 tttaaaaaaa gcaccggatt atttattagt tgatgcagca acaataccag gtattgacat
     3001 atctcaaaat ccaattgtga aaggagattc aaaatctata tcaatagcag cagcaagtat
     3061 acttgctaaa gttactaggg atagtataat gtaccaatat gatagagtat acccagaata
     3121 tgggtttaaa tctcacaaag gatatggaac taaagaacac tatgaagcta ttgaaaaata
     3181 tggcataact cctatccaca gaaaaagttt tttgaaaaat atactgtaaa tataatatga
     3241 tataatgtat ataatataaa ttactatagt taatggggga ttaaagatgg aatataatga
     3301 attattaaaa agtgctagag aaaatttaaa tggaagttgt aaagtttgta aggtttgtaa
     3361 tggggttgct tgtgcaggtg aagtgcctgg catgggaggt aaaggaagtg gttcttcttt
     3421 tatagaaaat agcaagagtt tagaaaaagt aaaagtaaat atgagagtaa tacatgatgt
     3481 atcaaatcct gatacatcta tagagatgtt tggaaagaaa atgagtgcac ctatatttgc
     3541 agctccagta acaggaacta ctttaaatat gggtggaaaa attaatgaaa gagattacat
     3601 agaaccagta gttgcaggat gtgctaatag tggtatttat gcaatggttg gtgatacggc
     3661 agtagatgct tttttaatgg aaaatttaga tgtagtcaaa aagtataatg gagctggaat
     3721 tgtatttatt aaaccttggg ataatgaaaa tattataaaa aagataagat tagctgaaga
     3781 agctggtgca tttgcagttg gagttgatat agatgcctgt ggtctagtaa ctttatcatt
     3841 acatggaaaa ccagtacttc ctaaaaatgt taaacaaata aaggagttag taaagtctac
     3901 taaactacca tttatattga aaggtataat gactgttgaa gatgctttaa tggcagtaga
     3961 agcaggtgtg tatgcaatag ttgtatcaaa tcatggagga agagtgcttg actgtacacc
     4021 aggagcatgt gaagttttac caaaaattgc tgatgctgtt aaaggtaaag ttacaatact
     4081 tgcagatggt ggagtgagaa ctggtttaga cgtactaaaa atgataggtt taggtgcaga
     4141 tgcagtatta ataggaagac cttttgtgac tgcttcattt ggtggtgcaa ctgatggtgt
     4201 agaaacttat gtaaataaac ttcaatcaga gctaagtagt tctatgatat taactggatg
     4261 tcaaactata aaagatattg atggaaaagt tatatacaaa taaaatttta tacaaaaaag
     4321 aggaaaaatc attttaatgt agaataattg tacaaagggg gaatttatat gagtagaaaa
     4381 gcttatgaag aagccttagt agaattagaa aaatttattg atgaaagaaa agaaataatt
     4441 aaaagtgcgg aggactgtat tgataagtac atagttgaca gaacgctacc ttttgattac
     4501 aaagataaat gtgtggaatg gcaacaagag ttacttgata ttgcagaggc acaagtttta
     4561 gaagcaaacg aattaggtgt tttactggaa gagaaaaaag aacttgagga agagtaagtt
     4621 ttattactaa atctagggaa aatcaagaat atgatttacc cctagatttt tttatttaaa
     4681 taaagatgtc tatataatta atctatcttt atcaatgtta gattaaaagg tttgaagaaa
     4741 aatagagtaa ttgtatgaat agttaaacaa ttaaactagt ctataagacc aatttaattg
     4801 ttattttttg aatttagtat atggtttttc atttcttcta atacaattgt gtaggatttg
     4861 cattgtgctg cagccaattc tgacaaagca aacctttgag ccttatttag aaagtcctta
     4921 tctgtgctac atagttgttt tgaattatct tgtatcataa gcatcttaat taattttaca
     4981 atctcaaaaa catctcctga atgaaattta tcatggtata gtttaagtcg tttttttcta
     5041 tcaaaaaccc ataaacaatc aaccttatca agggattgga taaagttttc aacttcattt
     5101 tcatccataa ttggacgtac accaatctta tcagcattta aaactggaat cgaaatttta
     5161 agattactat tgtaaataac agtcaattca tagtatttct gtatatgatg atttatatcc
     5221 tttgttacaa gaccacttat ataacaggct ccttcttttg gatacattac actttcaccg
     5281 attttataca tatggttata cctccaaact gaattttaga tattgcaatt tgggtacaat
     5341 gctttaatta tctaataatt aatattatat cacgaaatgc ttgacaagtc aaaaagatta
     5401 atatataata aaaagtttag aataatagat atatataatt ggctataaaa atataagata
     5461 agaggattta atatattaaa atatctagtt agtattgtat aaaataacat actaactaga
     5521 tttaataatt attaatttct agcaaaatct cttaagttaa gacttccggc tgttacaaat
     5581 accattccaa taagaattaa aacaattata tttattaata tattaccatt attattaata
     5641 gcatctatag tccaataaaa tggtgtaagc tttgaaagat tagccataaa ggataattta
     5701 tcaagtgatg gtatcatatt agatatggat atagctaaac ctattaaaga ataaaacgta
     5761 gacataagac ttatagaacc ttcgttttta aacaccctag ctatccaaaa tacaaatcca
     5821 gtagcaacaa aactatttgc tagtacaatc atcaatgtat ttagagtcaa tttaaaatta
     5881 cttatagatg tgaaacatac taaagttaag gtgtatacaa ttgcttgagt aaaaaataac
     5941 cctaaaaata tgctgaaaat tatttttata tcattatgct ttgtagaaac aagtcttttt
     6001 aaggtattag atttctttaa tgaaaataaa tcagaagcta gatgtgctgc attaatgtac
     6061 atgaaataac atatcaagaa aactattaaa aatgaatttt tattcttacc tgaactgtta
     6121 tcaattatat ttgtcttggt caatttatca tctatatttc tatctatttt taattttaat
     6181 gaattagtaa taaaagattc tatttgtgat tctgcccaaa gactacctcc accattttca
     6241 gttttaaaac atttaacctc aggtcgtttt aagttatcta tattgcttga aaagttttta
     6301 tctaaaatga agacagcaga aactttatta ttttttaata gatccatagc cttgtcttgt
     6361 tcgtttaatc caaatgagtt ttcttttaat ttagaactat ctattaactt atgttcgtat
     6421 tttccagaag aattggatac aatcgctact ggagaaaagt acattgattg attttctgag
     6481 ttgccagtaa aaaagttaag ataaaacatt aatataacag gaaacaaaac catattaact
     6541 aaaagagcat gattttttat atatcttttt atgtttgcaa tagtcaacct aatcattatt
     6601 aaacctccct ccatttatat ttttctttta caatagaaag tgcaatcaat actgtagatg
     6661 caattaggca tgtaaaaatt aaactttcta ttgaatctat agttttaaac gtctcataat
     6721 tactgaacaa tgttcctatt aaataagtag gagataaact tgtaaactta gtaaacacat
     6781 catttactat gctgaaaata cctccaaaaa ctgtttgcaa gaagagtaat gcataaacta
     6841 taactgtacc atatttcttg ctaaaaaagg tagttataaa agtagatatt gaagtcataa
     6901 aataagaagc taaagcacaa agtaaagcta atataacaaa gttacctttg aatgttacac
     6961 cgagtgttct aaaaaataaa acgtataatg ataaaaatat aaatgagtaa atccagaaac
     7021 ctataaaatc ataaattaaa aattgtactc tagttacagg tatagagtag agtctttttg
     7081 aaagtccttt tgattcactt acataatttg ataaaatgct attcattata aaaattattg
     7141 cgaagaatcc aagggaaact aaagcaaagt actcataaga gttttgttta acattatttt
     7201 ttattataga ggtatcaata gaattcttat taaataattt tgagaaatct tctgaactta
     7261 atttttgaga attattcaga tataattttt catgataagt atctaatata tcttgaagta
     7321 atattgcaat attatcatta gatttaagtt tttctatact tagattattt gatttttcat
     7381 ttaataaatt attctcatat cctttaggta tagtaagttt taagtctgcc ttagaatcat
     7441 ctttttgaac tgttaatata gaagataaat cgttttttag atatcctgtc aaattacttg
     7501 atagctttgt attatcttta tcatatataa caattgatgt caaatctgtt tttataggat
     7561 tttgaaatat actttctgtc accatcccaa gaaatccagc taaaactaga ggtaaggcta
     7621 agaaatatat taaattaagc ataaaggagc tttttaaaac ttttagcata gtttttacac
     7681 taatgtacaa tttcatgaaa tcacctccat tagtccctta aatttttacc tgttagattt
     7741 aaaaatacgt cttctaaact aggttcttca taagatattc gtgttatttt tgaattattt
     7801 ttgtcaacaa cagataatac atctgtaagt tgtaattttt tgtctacacc taattctaat
     7861 aatgaattat tttctaaaat agatacaaca cctttagtat tttttaaatc aaatataagt
     7921 tgagcattta tattttttat ttccataatt aattttgtat ttgtagatac taatgatttt
     7981 aaataatcat tatctccaaa agcaatctct ttaccttcat ccataataaa aatcttagaa
     8041 caaagatgct caacttcttc catataatga gaagtatata ttactgtggt tttttgcttt
     8101 ttagatatat tttttataaa attgaaaata tggtttcttg attgcgggtc aacacctaca
     8161 gttggttcat ccaatattaa tattttagga tgatttaaaa gtgctattcc aatgttcaat
     8221 ctcctttgca taccaccaga taattttttg atttttttct ttttaacatc agtgagtcct
     8281 gtgacttcta aggcttcagt tattctctct tgaagcaatt ttccctttag tccatagtaa
     8341 gtgccaaagt actctaagtt atcaaaagga gtaagagtct ctataagtgc taattcttgt
     8401 ggaactaatc ctattataga ctttgctttt atacaatctt ttgacaaatc taaaccattg
     8461 atttggatgt cgccactatt tggtaacata agatttgtaa ttatatttat aagtgtagat
     8521 ttaccagcac cattcggtcc aataagtcca aatatctctc cttctttaac agaaaagctg
     8581 atattatcaa gtgctaattt atcattaaat ctttttgtta tattatttac tcttactata
     8641 tccataataa ttcctcccaa aataattttg tataagttaa aaatctataa tactataata
     8701 aagaattatg aatcataatt ctattactat aagtcactaa aaaaagtgac ttaagtcact
     8761 ttatattttc atttattata aatgagtttg tgagtaagaa atttctaggg tatagatttc
     8821 ttacctatat atttatatta caattttatt tttgaaggca aatataacta gttgtgtcct
     8881 atcacgaagc tctagtttcc ctaagatatt ggtgatatta ttctttattg taccttcaga
     8941 taagaataat tcttggctga tttctttgtt gttaagtcca tttgcgatta atctaattat
     9001 ggatatttct ttttctgtta aattatacat atcagaaata tgtttttgtt ctatagtttt
     9061 ttgagggtac ataatttttt cagccacact tttatcaagt acaatatttc caaaaaacga
     9121 ggaacgaatc ccttcatgta atacttcatc cgaactatcc tttaaaagat atcctgaagc
     9181 cccaaatttc atagcatctt ctatgtactc atcatcatta aaggttgtca aaattaataa
     9241 tgctatagag ctatcatatt cttttataag tcttgtgcct agaactccat cacaaacttt
     9301 catccgaata tccataagaa ctatgtctac atcatttgtt ttacaaaatt ctaatgcttc
     9361 atatccattg gaagcatctc ctacaatttc taaatcgtca taagatgata aaataatgtt
     9421 aagacctttt ctaatcaatt tttcatcatc tactataaga actttgattt tattcaaaaa
     9481 aatcacctcc tgaaaattta ggaaaagacg ctctaattgt gaatccagta ttagaattat
     9541 cgaattcaac tttaccattt aattcatata ttctttcgct aatactgcta agtccatttc
     9601 ctctttttat attgttacac cctataccat tatcgctgat agtaattata agatttgaag
     9661 gattaaatgt tataaaaatt ctgatttcag ttgcttttcc atgtcttgat gcgttggaaa
     9721 gagattcctg aacaagtcta tataaagcga tagattgatt tgtagatagg ttccaggtat
     9781 tttttgatat agttaatcta acgttgatat ttgtaagttt aataaagcta ttaaccagtt
     9841 ctctgatttt aagtaaattt tggtaatttt catattcaat gggttttagt tcagatatag
     9901 ctcttctcac atcttgaaaa ctttcagtta caaactctct aagttcatga actaaatcag
     9961 aaatgagagg tttgtctttt aagagttttt ctatagcatt taattggatt atcgtcgtag
    10021 aaagagaatg tcccacacta tcgtgtattt ccctcgatat acggtttctt tctttcaaaa
    10081 ttgctaactc ttctatagaa tttgcataaa tctctaaatc ggcattagct ttttttaatt
    10141 catctttaga aaccctgagt ttgtcataaa gactttgatt gtataattta gataaattct
    10201 cgttttctag atacacagaa aagagggtta taattagtaa tgtagaactt gattctagtg
    10261 ctaaatataa atttttat
//
LOCUS       DAELXX010000127         9870 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.128, whole genome shotgun sequence.
ACCESSION   DAELXX010000127 DAELXX010000000
VERSION     DAELXX010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9870
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.128"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(419..2554)
                     /gene="rnr"
                     /locus_tag="KPH29_003166"
     CDS             complement(419..2554)
                     /gene="rnr"
                     /locus_tag="KPH29_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBG1468738.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILAKILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
     gene            3150..3764
                     /locus_tag="KPH29_003167"
     CDS             3150..3764
                     /locus_tag="KPH29_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBG1468739.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTSRDYFKSNPDVAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            3782..4144
                     /locus_tag="KPH29_003168"
     CDS             3782..4144
                     /locus_tag="KPH29_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1468740.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYKGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            4250..4747
                     /locus_tag="KPH29_003169"
     CDS             4250..4747
                     /locus_tag="KPH29_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HBG1468741.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
     gene            5171..5569
                     /locus_tag="KPH29_003170"
     CDS             5171..5569
                     /locus_tag="KPH29_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG1468742.1"
                     /translation="MIKKIEHVVITNNNIENYINFYKKFGFSSKEMSEKYKLKARNVK
                     INLNIKGYKFLPYVKNLKASRDNLCFKINTNIDEPKKYLEQNEIQIKRGIVECTGVFG
                     NMKSIYLKKTNGNLIELSNHRITESKPWKI"
     gene            5557..6495
                     /locus_tag="KPH29_003171"
     CDS             5557..6495
                     /locus_tag="KPH29_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBG1468743.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFKLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIRH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHIKTIILGVCGKLILVPSIFLPISIFLGFRNVELTSLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSIITVFLIIFILKQNNLI"
     gene            complement(6879..7103)
                     /gene="secG"
                     /locus_tag="KPH29_003172"
     CDS             complement(6879..7103)
                     /gene="secG"
                     /locus_tag="KPH29_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBG1468744.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
     gene            complement(7221..7727)
                     /locus_tag="KPH29_003173"
     CDS             complement(7221..7727)
                     /locus_tag="KPH29_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG1468745.1"
                     /translation="MNLEIIRATSENAIEMGFIHSCSWRKAYKGIIPDKIIDEFTSEK
                     RAEVFQSVIPKAEEEYYLFKVNGIPAGFASLNRSQEDNAPKCVGEIYSIYFHPDFWGT
                     SVTKKGLQFCIDRLSNLGYSYITIWVLKDNTRAINFYKKNGFTCDDFEKEIHIGKKLL
                     EVRYSKKI"
     gene            7812..8240
                     /locus_tag="KPH29_003174"
     CDS             7812..8240
                     /locus_tag="KPH29_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG1468746.1"
                     /translation="MNPVKELNNLWHIVSMQMKTEMCSGKYPTIHGISLVELSILEIV
                     EQYPNSMMKEISEILELPKSTLTSAVKRLELKKFIKKNPCIKDKRRYNLVLTELGLKA
                     QQEHRDIEIIVFRNLLHQLDKDEIETFIRIFSKAVNKSKK"
     gene            complement(8360..9652)
                     /gene="eno"
                     /locus_tag="KPH29_003175"
     CDS             complement(8360..9652)
                     /gene="eno"
                     /locus_tag="KPH29_003175"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBG1468747.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTERLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
BASE COUNT         3324 a         1519 c         1182 g         3845 t
ORIGIN      
        1 tattaaacat ttcttaatta cttatttttc aattagccac ttctattaaa catttcttaa
       61 ttacttattt ttcaattagc cacttctatt aagcatttct taattactta tttttcaatt
      121 agtcacttcc attaaacatt tcttaattat ttatttttca gttggctttt tctattaaat
      181 gtttcttaat tacttatctt tcagttagct ctttctatta aatatttctc aattacttat
      241 ttttcaatta gctactttca ttaaatattt cttaattatt tatctttaag ttaactattt
      301 ccattagata tttcttaatt acctattttt atcatttatt ttatagagta ttgtgcacta
      361 tatatttata catatcataa atactacaac ttgttatctc ttattttagc tgttttatct
      421 actcctctat tttttcaact atctcaaagt caatttcttt aaaatctaca ttaacacttt
      481 caacctttat cttaacaagg tctcctatct tatatgactt cttagttctt tctcctaaaa
      541 cactataaga actctcatca taaatatagt aatcatcatt catatttgca agtcttatca
      601 atccttctat agtattatct aactcaacaa atattccaaa tgaagttacg ctagatacaa
      661 caccctcaaa ttcttgacca actttatcct tcatataaag agccttatag aaatcatgta
      721 catctctttc agctaactct gcctttcttt ctttctcgga tgattgaaca gatgcataat
      781 ccactatagt agtaagctgt tcttgtcttt ttccacttat tttattattt aaagattctt
      841 ttattattct atgaatttga aggtcaggat atcttcttat tggtgaagta aagtgacagt
      901 agaatttagc tgctaatcca aaatgtccac tacattctgg tgagtactta gcttgtttta
      961 aagaacgaag cattatagta ctgatagctc cttcttcttt tttgcccttt attttctcta
     1021 ttataccttg taatgctttt ggatgtactt cctctttatc accttttatt atatatccaa
     1081 atgtagatat aaacttactc aattcttcca ttttttccaa agatggtgtc tcatgtattc
     1141 tatacacaaa cggtattccc atccagtaaa aatgttcggc aattgtctca ttacttataa
     1201 gcataaattc ttctattatc ttattagata ttcttctttc atatggcttt atatctacaa
     1261 cttcgccttt tccatttaga attatctttg cctctgggaa attgaagtct atagctcctc
     1321 ttttttctct tcttgacata agaatcttag ctaaaatttc agcattttta aaatcttcaa
     1381 caacatgttc aaaagtcttt ttaagttttt catcatcctt ttctaatata tcagatactt
     1441 ctgtataagt cattctagct tttgaattta ttacagattc acatatttca tgtttaacca
     1501 catttccttt atggtctatt tccataaata tagaaagagt taacttatct tcaaatggat
     1561 ttaagctaca tactccattt gaaagagttt ttggcaacat tggaattact ttatctacta
     1621 aataaactga tgtagctctt tttaaggctt ccttatcaag tttatttttc tctcttacat
     1681 aatgtgtaac atcagctatg tgaactccta gtttgaagtt tccattaggt aatacttcta
     1741 ttgatatggc atcatctaag tcttttgcgt catctccatc tatagtaaat atgtttaaat
     1801 ctcttaaatc tcttcttctt tttatctctt cttgaggaat ctcaacagct acagcttctg
     1861 cttcttgcaa tactttcttt ggaaattctt ctggaagacc atgttctctt attatagaat
     1921 ctatttctac gcctctttca cctttttgac ctagtatctc tataattttg ccttctggtt
     1981 ttctatcctc ttgtggccat actgtaattt cacatactac cttatcatta tctactgctc
     2041 cactaaaatg ttttttaggt atatatatat ctttagtaaa tttcttgttg tctggtgtta
     2101 caaatccaaa agttttactt gattggaaag tacctactat tctagttatt tctctcttta
     2161 ttacttttac tattcttcct tctgctctct tttcttcagt tgcaggtact actatttctg
     2221 ccataactct atcgttatgc atagcaccat ttatattgtc acttgagata aataaatcct
     2281 gtgtatattc aacatcagat tctacaaatc caaaaccctt tttgtgtgat acaaattttc
     2341 caacaaagta tcccatttga ttgggagata ctatttttcc ttttttagta aagcagatat
     2401 aaccatcttc ttctaattca tttaaaaaat tataaaacat tggcatttct gatgaatgta
     2461 tatcaaatat ttctgctaat tcttcttttt ttagaggatt atatgcactt tcatttatta
     2521 atcctagcaa actttcttta agtcctggta tcatttttcc ctcctgtttt acttttcatt
     2581 atattattat ttatattgtc ttttatattt cttcagataa aacatttttt acttatcaca
     2641 taattttcta tattttctta ttattattac aagttaaaat tacttattta tatttttgat
     2701 ttaatgtaag ctttgtcata ctaacttcaa acaaattatg atgttttata aattatttgt
     2761 aaatatcatt ataattactt aagttattct ttatcataat tgcttaaatt actctttatc
     2821 tcataattat acaccatcaa ataggattgg taaaatattc atctcatata agtattacta
     2881 tataataatt tattctatta gtatactcaa atcatgtaaa atttatataa ctcaattaat
     2941 tttttaaata caactttata atataaaact tataataaaa gtaaatttat atgttaaatg
     3001 aggttgattt tttattattt tttatttatg ctagtactaa agacttgcaa acgttttttg
     3061 acaagtcttt attttgtcta aattaaacaa acttttttac aaataaatac ataaaattta
     3121 aactaatcta tttattggag gagataaaat tggaagaagt aattgcaaaa attattacta
     3181 tagaaaatgg attcaaagaa attgaaaaca ttgctaaaaa aattgtaaaa aataatgact
     3241 caaaaaagtg ttattatcta tcaatctact tatataggtc agaatattat caagtaagag
     3301 aattggctgt gtttatactt ggttttatat cagtaactat ttctgaagct ttgttatttt
     3361 taaaagacaa tgtgagtaaa gatgaaaact ggagagtaca agaaatacta gctaaatcat
     3421 ttgattatta ttgcagtgaa gtaggatatg aaaaggcact tcctgttata aaagaatggc
     3481 ttaattctga tagtccaaac ataaaaaggg ctgtgacaga aggtcttaga atttggacta
     3541 gtagagacta ttttaaatct aatcctgacg ttgctatatc actactttct agtttaaaag
     3601 aagatgatag cgaatacttg agaaagtctg taggtaatgc tcttagagat ataagtaaaa
     3661 aatatccaga tttagtaaaa aaagaattag attcatggga tatatcaaat aaaaaagttg
     3721 agtttgtata taagcttgct tcaaaatgta tcttggcaaa gtaaaatgga ggtgaactta
     3781 tttgaaactt cgtgatgagt atacttgccc attagaacta acacatgata tgataaaagg
     3841 caaatggaaa tctattatat tatggagatt acgcatagga ccaacctcct tatcaaaact
     3901 tgaaaaggaa atcaatggta ttaatcaaaa gatgttactt gaacaactta aagaattaat
     3961 tgaatttggt cttgtagata aaaagacata taaaggttac ccacttaaag ttgaatactc
     4021 acttacacct attcgaggga aacaaatatt ggatgctttg gctataatgc aagatatagg
     4081 aattagcttt atgttggata atggtatgga agatattctt aaggaaaaag gtgttttaaa
     4141 ataaatagat acccacaaaa aagtaagtaa tttaccatta ttttcaactg gtatataatt
     4201 aaattataga tatattctat taatttatta taataggagg taaattatta tgaaagtaac
     4261 tagtacaggt atagtaaatg gtgttataga agataaatac ggtaaaagag gaactcagtt
     4321 caatgaaggt ggtatgccta catattcact acctctaaag tttgaagatg ctccaaagaa
     4381 tacagtatcc tttgctgtat ttttagaaga taaagactct gttcctgtat gtgggtttgc
     4441 atggatacat tggttagctg taaatataac aaaagatgag ttaaatgaaa atgaaagtgt
     4501 aacagctact gattttgtac aagggactac tagttggcat ggcaaactag gaggtcttga
     4561 cagattagag gccagtgtat atggtggaat ggctcctcca gacagacctc atgtctatga
     4621 aatacatgta tttgcacttg atactatcct tgaccttgaa aaaggattct atatgaatga
     4681 atttttcaaa gctattgaag ggcatgtttt agatacattt acactaaaag gaagctattc
     4741 taattaatta ggtttactat agctaatcat taattaaatg ctacatttaa ttgatgatta
     4801 gcttaattgt tattagaata ttttctgcac tttatcttaa tatatatttt agttgactgg
     4861 tattttaatt tctattagat tattatcaat ttatataaat tgattttagc ttaaaactta
     4921 cttatctatt tcagtttcta ttagaattat tattagactc attaaaataa aagcattaat
     4981 ataactaata atatatgtta aattcattaa ctctacataa agttttataa tctaaatgaa
     5041 atacaataat ctgcataaaa ttcaatagct tatataaaat ttagtaattt atataaatat
     5101 tatcaatcta tataaaattt aataacttat ataaatttaa ctaatttata taaaatctta
     5161 aggagttgcc atgataaaaa aaatagagca tgtggtcatt actaataata atatagaaaa
     5221 ttatatcaat ttttataaaa aatttggttt ttcatctaaa gaaatgagcg aaaagtataa
     5281 acttaaggct agaaatgtta agataaatct taacataaaa ggttataaat ttttacctta
     5341 tgttaaaaat ttaaaagcaa gtagagataa cttatgtttt aaaataaaca caaatattga
     5401 tgagccaaaa aaatacttag aacaaaatga gattcaaatt aagcgaggaa ttgtagaatg
     5461 tacaggtgtc tttggtaata tgaaatctat ttatttgaaa aaaactaatg gaaatttaat
     5521 tgaattatca aatcatagaa tcacggagag taaaccttgg aaaatttaat cctatcaata
     5581 aatgttgttc ttcctttgtt tctcacaatg tcacttggat atgttctaaa aaaattaggt
     5641 ctatttgata ctcatacttt aaataaaatg aataatgtct gcttcaagtc tttcttgcca
     5701 ttattattat tttttaatat atacaataca gatttagatg gttctcttaa ttttaagctt
     5761 atggctgtag ctgttataat tattttttca atcttcttaa tcttagtctt tattattcct
     5821 aaaattgaaa aagataataa gacaaggggt gcattaatac aaggtgtatt tcgtagcaat
     5881 ttcgttatat ttggtattcc tgtgacaatt tcactatttg gcgataagag tattggagtc
     5941 acatctttat taattgcagt tattgtacct atgtttaatg tattatctgt tgtaattctt
     6001 gaaattttta gaggtggcaa agtaaatata agacatatga ttaaaagcgt cttaacaaat
     6061 ccactcataa tcgcctcttt tataggattg acaatgctaa ttttaaatat taatatacct
     6121 acatttttac acaaaactat tggtgatatt tctaaaatag ctactccttt atcacttata
     6181 attctaggcg gatcttttag tttttctgct ataaaggggc atataaaaac cattatattg
     6241 ggcgtatgtg gaaaacttat acttgtacct tctatatttt taccaattag catttttctt
     6301 ggatttagaa atgtagaatt aacttcactt atgataatgt tatctgctcc aactgcagtt
     6361 tcttcattta caatggcaca acagatggat tctgacagtg aattagctgg acaaattgta
     6421 gtatttactt ctggattttc tataattaca gtatttttaa taatatttat tttaaagcag
     6481 aataatctta tttaatgata tttgataaca caaattggta ttatgagaaa catatataaa
     6541 attgatttaa ttgcgtaaaa acatattatt atactatttt tactatataa tataactttg
     6601 tgtatattat ctcattaaac atggtgaaag tagtgttgct atatattttt tagtttgaaa
     6661 aaaatatata aatataaaca atatataaat acaaataata aatgccaaca aatagtcaat
     6721 ctataaaata aaaactggac taatcatata aaatcattag tccagctttt attttacgta
     6781 ctttttatgt tgatgtactt ttattaaaaa ctaaatttaa tttttaaata tctagcaaat
     6841 gcactaatta aataatggcc agttattcaa caatatcact acttcttaac tataagtaat
     6901 gccaatgcat ttataaaaaa taatactgct gttatctttg taactctagc aagaaatgct
     6961 tgttttcctt ttggtttaaa gtaagcttga cttccttctc caccaaattc tgaaggtaaa
     7021 gcattttttg aatcttgagg cattatgcta attactagta aaatccctag cactacttgc
     7081 acacccatta aaatgttgct cattattgcc cctcctatct atttattact taaatttcat
     7141 tactctttag tgtatcacaa taatatagtc atttcaagac tattgaagta taaataacaa
     7201 acaaaaataa tatgcaatta ctatatcttt tttgaataac gtacttctaa taattttttt
     7261 ccaatatgta tctctttctc aaaatcatca caagtaaaac catttttttt atagaaattt
     7321 atagctctag tattgtcttt tagaacccat atcgttatat atgaatatcc taaattgctt
     7381 aatctatcaa tgcaaaattg taatcctttt tttgtcacag atgttcccca aaaatcggga
     7441 tggaaatata tagaatatat ttctccaaca cattttggag cattatcctc ttgacttcta
     7501 tttaatgaag caaaaccagc aggtatacca ttcactttaa aaagataata ttcctcttca
     7561 gcttttggaa taacactttg aaaaacttct gctcttttct cagaagtgaa ttcatctatt
     7621 atcttatctg gaataattcc tttatacgct tttctccacg aacaagaatg tataaaaccc
     7681 atttctatag cattttcact tgtagctcgt ataatctcta aattcataaa aacaccatcc
     7741 tattgacaaa ttaatacgat tatcgtacta tattagtgcg ataattgtat catatatgga
     7801 ggtaaaagtc aatgaatcct gtaaaagagc taaacaacct gtggcatatt gttagtatgc
     7861 agatgaaaac agaaatgtgt agtggtaagt atcctactat tcatggtatt tctttagtag
     7921 aattaagtat tttagaaata gttgaacagt atcccaatag tatgatgaaa gaaattagtg
     7981 aaattttaga attgccaaaa agcacattaa caagtgctgt aaaacgtctt gaattaaaaa
     8041 aatttattaa aaagaatccc tgtattaaag acaaaaggag gtataactta gtcttaactg
     8101 aattaggttt aaaggctcaa caagaacata gagacataga aataattgta tttagaaatc
     8161 tcttacatca attagataaa gatgaaatag aaacttttat tcgtattttt tctaaagctg
     8221 tgaataaaag taagaaatga aatataaaaa aaggataagt gaattgaaaa atcaactatc
     8281 acttatccct tataagataa atttttatct tttatgttat ctttttaatc tttaatgcca
     8341 tatttttaat cttcaataac tattttttta aattatagaa agattttaat ccacagtatc
     8401 tagcttgttc tccaaccatt tcttctattc ttaataattg gttgtactta gcaactctat
     8461 cagttcttga tggagcacca gtttttattt gaccagcatt tactgcaaca gctaaatctg
     8521 ctatagtaga atcttcagtt tctcctgatc tgtgagatat aacagcagta tatcctgctc
     8581 tcttagccat ttctatagca tctaaagttt cagttattgt acctatttga ttaactttaa
     8641 ctaatataga gttagctact ccattttcaa ttcctttttc taatctctct gtgttagtaa
     8701 caaataagtc gtcaccaact aattgtaatt tatttcctaa tctttcagtt aataacttcc
     8761 atccgtccca atcttcttcg tctaaaccat cttctatagt tattattggg aagttgtttg
     8821 accaatcttc atataaagca accatttcag cagcagttaa ctcttttcct tctcctgcta
     8881 aaacgtattt tttagtttct ttgttgtaca tttctgtagc agcaacgtca agtcctaaca
     8941 taacatcttc acctggctta tatccagctt ttgttatagc ttcaactatt aattctaaag
     9001 cttctctgtt tgaacctaag tttggagcaa atccaccttc gtcaccaaca ccacaagcta
     9061 atcctttttc tcctaaaact ttctttaatg aatggaatac ttctgctccc attcttaaac
     9121 cttctttaaa gcaacaagct cctactggta atatcatgaa ctcttgaaca tcaacgttat
     9181 tatcagcatg ctctccaccg tttaatatgt tcatcattgg aacaggtaat tgtttagcat
     9241 ttactccacc taaatattgg aataatggta aacctatttc atcagcagct gctctagcta
     9301 ctgccattga aacacctaat atagcattag ctcctaattt acctttgttt ggagttccat
     9361 ctaattctat catgattgca tctatagctg gttggtcaaa tgcatccatt ccttctattt
     9421 caggtgctat tatttcattt acgttagcaa cagctgtttc tacacctttt cctaagtatc
     9481 ttcccttatc tccatctctt aattcaacag cttcaaaagc tcctgtagat gctcctgatg
     9541 gaactattgc tcttcccatt gctccatctt ctaatactac ttcaacttct acagttggat
     9601 ttcctcttga gtctaatact tctctagcat atactaattc aataactgac atttgtatat
     9661 ctcccttcga ttttctatta gttaaatttt catttactgt aaacatccgt ctactatttt
     9721 aatctacttt ctatattata gtacataaag ttgcttttga aaattaggta caaatttatt
     9781 tgatgggtaa atttgtaaaa gttcctattt ttattttatt ttttataata ttttaattta
     9841 tataatataa aaaaacttgt ctaaaatcca
//
LOCUS       DAELXX010000128         9868 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.129, whole genome shotgun sequence.
ACCESSION   DAELXX010000128 DAELXX010000000
VERSION     DAELXX010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9868
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.129"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(413..1066)
                     /locus_tag="KPH29_003176"
     CDS             complement(413..1066)
                     /locus_tag="KPH29_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG1468748.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKFMRENDLENVERVLREHWKTIPIAEMKKEKLL"
     gene            complement(1311..3176)
                     /locus_tag="KPH29_003177"
     CDS             complement(1311..3176)
                     /locus_tag="KPH29_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HBG1468749.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDIPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIKIRNKNKLLSSGNTEFIYTNNDSVFSFIRVDENNDRMLILVN
                     RSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN"
     gene            complement(3181..5601)
                     /locus_tag="KPH29_003178"
     CDS             complement(3181..5601)
                     /locus_tag="KPH29_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HBG1468750.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     AEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNIDNLFDKVAIHINDTHPTLCIPELMRILL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEEINRRYVE
                     ELNNKGYEQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            complement(5615..7057)
                     /gene="glgA"
                     /locus_tag="KPH29_003179"
     CDS             complement(5615..7057)
                     /gene="glgA"
                     /locus_tag="KPH29_003179"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBG1468751.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNRDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            complement(7086..8216)
                     /gene="glgD"
                     /locus_tag="KPH29_003180"
     CDS             complement(7086..8216)
                     /gene="glgD"
                     /locus_tag="KPH29_003180"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HBG1468752.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGS
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            complement(8218..9378)
                     /locus_tag="KPH29_003181"
     CDS             complement(8218..9378)
                     /locus_tag="KPH29_003181"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBG1468753.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKRSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
BASE COUNT         3134 a         1758 c          947 g         4029 t
ORIGIN      
        1 tagtatttat aaactttctt attttttatt attttgcttt ttttactttt ttgagtttta
       61 gtaagctttt gagtttacta tttttaattt ataagtattt tattactaaa cttttatatt
      121 attcttattt catatatcaa aattcttggt ttagttggac taaataattt tcctattttt
      181 gtattttaat tttaatataa aatttattaa attttttaat tgataaaata aattctatat
      241 ttatgatatt aaaattctat aagttcattg atttatatat gttaatttaa atatattatt
      301 ctatcgtagt gccttagagt ttttatttgt atttaaagtt aaatttattc ttgtttttcc
      361 tttaatattt aatagtaaaa taacaaaatt ataaaaaaaa taaagagaaa ctttataaaa
      421 gcttctcttt tttcatctct gctataggta tcgttttcca atgttctctc aaaactcttt
      481 caacattttc taaatcattc tccctcataa attttactac ttcaatatgt tgattattgc
      541 tgtatgtcaa cattttatat aactcatctt cattatcact agtatatgac tttgacataa
      601 aattatatct taaagagttc aatagattta ctaacacctg attatctgat ttagttatgt
      661 atatatcgtg aaattcatta cttaatttta aatagctatt gtaattttta tactttatag
      721 agaccgccat catttctgtt aattcttcca tttttactag gtctgattca gttaaatttt
      781 gtaatgctga agaaccggct aacgcatcca atactccaat tacggaatag gtgtctaatt
      841 tctccttttc ctgaaattct tttactacaa atcccttatt aggtattttt tctagtaaat
      901 tttcacctgc taacctagtt aatgcttctc tagctggtgt tctactaact ccgatagatt
      961 tacaaatctc agattcagta attctctgat tagcttcata ctctccattt tggattttct
     1021 ttgatatata gtcatatact tgagttgtaa gtggatctac cttcataaga cgttcctcca
     1081 tattaagatt ataaaagtta aatctcccta caactttggt agagagttca gttttatctt
     1141 actaatttat ataatatcac aattatgata ttttttatac ttctttgtaa tattttgaaa
     1201 cactaaaatt cttatgataa tttatacaaa aaaagtagaa aaagttttga taaaacaaaa
     1261 ctctttctac attcacctta ttttctattt atttttctaa aaaaagatat ttaatttact
     1321 ctaaaaattc taaatgattt agagtctaaa tctactttca actcattatt ttcaatttgt
     1381 atagtactat ttgcattttt aagactatac tttataggta tttcctcaat aaaactactc
     1441 tctatacata atgatatact ttccacattt tcacttctat ttactaaaat taacattcta
     1501 tcattatttt catcaactct aataaaagaa aatacagaat cattattagt atatataaat
     1561 tctgtatttc cagaacttaa aagtttattc ttatttctaa tttttataac atctctataa
     1621 aaattaagca tatcttcgtc ttcatttttc catggataag ttcttctatt atctgggtca
     1681 acacctccac aaagacctgc ttcatctcca taatatatat atggtactcc ttcaaatgtc
     1741 atctgtatag ctacagcaag cttcactaaa tctacatcat catttaattc cgttttagct
     1801 cttgtaacat catgtgttcc aattaaattt aaattagacc taaatgattc ttttggataa
     1861 ttttctttaa tctgcatata tctattacaa agttcatatg aattaatttc tccttttaag
     1921 aaagaaaaca tgttatttct aaaaggataa cccataacag aatctaattc ttcacctaat
     1981 aaataacttc ttctctgacc atagctaatc ttatttgatg catcttccca tacttcacct
     2041 ataagaattg agtcatcatc tgcctttttt aattcctttt ttaactctct tataaactta
     2101 gttggtaatt cgtctgcaac atctagtctc cagcccttaa tacccatatt tacccattta
     2161 tttataacgc tatcattgtc atatattata taatccatat agctatgttc taattcattt
     2221 atatttggca atgtcttaac atcccaccaa cttttatact tttgaggaaa ttcttcaaac
     2281 atataccatg aataatatgg agattcttta gattgatatg cccctaaact attatagttt
     2341 ccatacatat taaaatattt actatctgct ccagtatgac taaaaactcc atctaatact
     2401 attgatatac ctttttcttt tgctttatca ataagttctt taaatgtatc ttcatcccca
     2461 aacattgggt caattttttt atagtcacct gtgtcatatt tatgattact agaagcttca
     2521 aatatagggc ttagatatag tatacttaca cctaattttt ttaaatatcc aagtttattg
     2581 attatccctc ttaaatttcc accatggaaa tcccatctta taacatcccc ttgactatcc
     2641 tttatgtaca taggtatatc ttcccaattt ccatatatga aactattttt tttaggatta
     2701 tcaaccttac cactacgatt tccattattg aatctatcta caaatatatg ataaagtatt
     2761 ccttctttgt accatgttgg tactttaaaa tctttgtaaa cagtgatttg atatttatta
     2821 atatcactat aattatattc acaagaatat ccattatctc tgttttttcc ataaaataaa
     2881 gtctttatct gaccatccat ttcaacatca actttaaagt aataaaaata aacacctgta
     2941 ccatcaaact tttcaatctt accacaaaag cactttccaa gtttatcatt atctaattct
     3001 tcatttaaag ttattgttct catttcttct tctctattta tttctaggga aatagacttg
     3061 atattgtatc cctcatttac tttaacacaa atggtcaatt cctcatcaaa ttttaaagct
     3121 ccaaacggag ccttaaaatt tttatcccaa gagttatatt ctattacact ttgcatttaa
     3181 tcacaccctt ttaaaccaaa tgtcttttgc atactcagaa attgttctat cagaagaaaa
     3241 aaatccagag ttagctatat taactaaaga cattttattc catttttctt tatctctata
     3301 taacctgttt atatctgctt gagcttgacc ataattttca aaatctctca atacaaaata
     3361 ctcatcattg taagtagtga gtgaattata aatacttctt ccttcttcgc caagattagg
     3421 aataaatcca tttattaaat catctaccac cctttttata tccatattag aatgatataa
     3481 gtcaagtgaa gaatatccac catatttatt ataatttagt acttgttctg cactaagtcc
     3541 aaataaaaac atattttctt taccaacctg ttcacatatc tctacatttg cgccgtcaag
     3601 tgtagccaaa gttatagcac cattcatcat aaacttcata ttacttgttc cagatgcttc
     3661 cttagttgta gttgaaattt gctcactaac atttgctgct ggtattatta tttctgctaa
     3721 tgatactcca taattctcta aaaatactac ttttaacttg tcctttactc taacatcatt
     3781 atttatagta cttgcaacag aatttataaa tttaattata catttagcca aataatatcc
     3841 tggtgctgct tttgcaccaa atataaaagt tcttgggtca agattcaaat taggattatc
     3901 aagtaattca tgatacatat gaagtacatt aaatatattc agtaactgtc ttttatacgc
     3961 atgcaatctc ttaacttgaa catcaaatat cgaacttggg tctacattta aatcgtattt
     4021 atcttttata aactttgcta gatttgcttt attattgtat tttatatcat caagtctttg
     4081 taaaacagag gaatcatttt taaatttttc aatatttttt aattgtaagg tatccttctt
     4141 ccatgaatca ccaattaaat ctgtaattaa attgctaagt tgaggatttg aacttataag
     4201 ccatcgccta tgtgcaattc cattagtctt attattaaat ttatttggtt catcttgata
     4261 gaaatctttt aatacctctg tttcaagaat atgagtatga agcttagcaa ctccatttac
     4321 actatgactt gtgacaatac ataagtttgc catattgata ttgtcacagt ctattatact
     4381 cattcttttt attctatcct gctcatatcc tttattattt aattcctcta catatcttct
     4441 atttatttct tctattatca tataaattct tggtaatagt tctttcatca tacttacagg
     4501 ccatttttcc attgcttctg acattatagt atgattagta taggacactg tttttttagt
     4561 tatttcccat gcttcatccc aagataaatt ttcttcatca agcaaaattc tcataagttc
     4621 tggtatgcat aaggtaggat gtgtatcatt tatatgaatt gctaccttat cgaataaatt
     4681 atcaatattt aatttgttct ttttatattt tcttattata tcttgtatcc ctgcacttac
     4741 aaaaaagtat tcttgtttca acctcaacaa cttacctgca tagtttgaat catcaggata
     4801 taatacctgt gatatttcct cagtataata cttatgtttt aaagcctctt catagctacc
     4861 agaataattt agagcatttg aggtaagttc accaaaatct ctctttggta tttcactctt
     4921 aaacaatctt aaagtattta tacattggtt ttggtatccg attattggta tatcataagg
     4981 cattgccatt acaggaaggt agtttttatg aattactttt aaatgactac cttccttgat
     5041 taattctacc tcaccaccaa acttcaccat tgttgcttca tttggtctta cagtttccca
     5101 tgcatatcgt ccttctgtaa gccagttatc aggcacttct acctgataac catttacaaa
     5161 tttttgttca aacagaccat atttatacct tattccatat ccttgaccac ttatgttcaa
     5221 agatgccata gaatctaaaa agcaagctgc taatctacca agtccaccat ttcctaaggc
     5281 tgggtctact tcagcttcaa ttaaatcatc caagttgatt cctaattcag ataaaccttc
     5341 tctaacttct tcttcaatat ttaaatttaa taaattagat tttaactgtc tccctattaa
     5401 aaattctaaa ctaaaataat aaacttcttt tttcttatct aactttgtag caacccattt
     5461 ttttgctatc tcatctttca atagactaca taatacattt aacaattgtt catcagtagc
     5521 ttccttgata gattgtgcat ataagctata catttttact tcaaattttt gcttgaattt
     5581 ctttttgctt attgttacca aaaaaaccac tcctttaatc acatatgtct ctatatgttt
     5641 ctatatactc cttagctgat tttttccaac tattatctgt tttcattgca ttttcaacta
     5701 atttaatcca tttttctttg tccttaaata ctttgattgc atttttcaaa cagaaaaaca
     5761 tctcatgagc attgtaattt ttaaaactaa atccattgcc ttctccagta aatttattat
     5821 aaggtgttac tgtatctcta agccctccag tctctctaac tattggtaaa gaaccatatc
     5881 tcatagctaa catttgacct ataccacaag gctcaaataa tgatggcatt aagaacatat
     5941 cacttgcagc atatatctgt tgagccaatg atgcattaaa agtaattcta gctgatactt
     6001 tatctgaaaa attagaatcg taatagttga acattgattg atattgatgt tctccagttc
     6061 ctaaaacgac aatctgtaaa tcttctctca taagttctgg catcatgtat gctattaaat
     6121 caaatccttt ttgagaaact aatctcgaaa ctatacctat caacggaata gatgaatctt
     6181 ttttaagtcc caatatatct tgtaatctca atttattttc tacttttttg tcaattgagt
     6241 ctacatcata atgaacaaat atatctttat cttttgcagg gtcgtttaaa tcataatcta
     6301 taccatttaa tatacctttt aattttcctg actcttttct aagtaatcca tctaattgtt
     6361 caccataaaa agatgtttgt atttcatttg cataagtagg actaacagtt ataattttat
     6421 ctgcaaaatt tataccagct ttcataaaat ttattgagcc ataatactct atatctctat
     6481 tatcaaagta tttttgtggt aagtccaata tatcatataa aacatgctta tcaaatacac
     6541 cttgatattg gagattatgt atagtataca tagtttttat atttttatac ccctcaaggg
     6601 ttgcatatct ttcttttaga attaatggta acatccctgt atgccaatca tttatatgta
     6661 ttacatctgg ataaaaatca attctactta tagcttctaa aactgcatct gtaaagaaag
     6721 tatatctttc tgcatcatct ccatatccat agatactctt tctttcatct tctcttctaa
     6781 aataaaattc attatctata aagtaaaatt ttacaccatc caattccatt tctaaaagac
     6841 cacagtattg atttctccat ccaacacgta cactaaacac agctatttct tttagttgat
     6901 cttttaaata acttggtata gttgaatatt tgggcattat tacccttaca tctacacctt
     6961 ctttttttaa ttctttaggc aatgcataag aaacatctcc taatcctcca gtttttgcaa
     7021 agggccaaca ctctgctgtt acataaaaaa ccttcattag tcattccccc ttctaaaaaa
     7081 tttatttata tatttgtatt cttttccact acaagaggat atttttcatc tcctcttaat
     7141 tctttcccat ttgatacact cgtattttta tctaatattg cattatatag ttttgcattt
     7201 gactttatta cacaattttg cattactatt gagttctcta taactgttcc tttttcaaca
     7261 tttacctttc taaatatgat actatctttt acagtacctt ctattaaaca accagatgct
     7321 ataaagctat tttctacttt agcactatct gtataaattg taggtgattc attcttttct
     7381 tttgtaaata tctttctttc agagtataat aattcatctg caacttctaa atttaataaa
     7441 tctttgctca tttcaaaata tgattcaaca gaatttacac attttagata tcctgtatat
     7501 tcataagctc ctattttcat attttctgct tcttcaataa taaattcttc taaataacaa
     7561 taactgcctt tatttacaat gttatatata cattcaacaa aatcttcacg cttcataata
     7621 tatgtctcca tacatatgtt tgctctaggg aatttgccta aattatttcc tacatttatt
     7681 accctattgt tactatctaa atttagtgtt aagttaccag caaaatcttt atttgcatta
     7741 tttacatttt tatatattat agttatatca ttatgagatt ttttatggta ttccaatgct
     7801 tgagaataat ttaaattgca tagcatatag cttggagcta ttaaaatata ttcttcttca
     7861 ctcttttcta tataatctag attagctaag atagtatata tatcaccttc tctatgtgag
     7921 taagtatttc tatacttagt gttttctgga ctaaaaattg ataggccacc tttttttcta
     7981 cttaaatccc aatctttacc actacctata tggtctgtca atgatctgta tttttctttt
     8041 gcgaatattc caactttagt gattcctgaa tttatcatat tagataaagc aaagtctatt
     8101 attctatatc ttccagctat tggtgtagat gctattggtc ttccatagtt aagtttgttt
     8161 attgctgggt ctcctttttt gtttaaattt attatcccta aacattcatt tctcatctta
     8221 aagacctcct tctattaata cgcatctagg ttcgatattt tcatactcag aaactacatt
     8281 tatttcatta tttacattct ttatctgaac attatcattt atagttgcac actcacctat
     8341 catagctttt tctatagtta cattttcacc tattacaaca tttggcatta ttactgaatc
     8401 aattacttta cttcctttac caacaacaac tccatgtgat aatacagagt tgcctacttc
     8461 acctaaaatt ctacaaccat cagctatcat tgatctatga acatttgcat ttttacctat
     8521 atattgtgga ggcattgcca tagtatttgt atatatcttc catttagggt ctgctaaatc
     8581 aagtgtttct ggagatttta ttatatccat attagcatcc catagacttt gtatagttcc
     8641 tacatctctc cagtatcctt taaatggata agcatacatt ccaacattat cttctaacat
     8701 tttaggtatt aaattttttc caaaatcatc ataatttatt tctggatttt tttctgcttc
     8761 cttaaaataa tttctcaaca ttttccaatc aaatatgtat actcccatag atgccaaatt
     8821 gctcttaggt tcacttggtt tttcttcaaa ttcatagata cttgaatctt catttgtatt
     8881 cattatccca aatcttgaag cttcatccca aggaacttca atcactgcta ttgtagcttt
     8941 agagcgtttt tccttatgaa attttaacat tttgctataa tccatcttgt atatatggtc
     9001 accagataat atcaatacat attctggatt ataggtatct acaaagtcca tattttgata
     9061 tatcgcatgt gctgtaccat tataccaatt tccttctttt tcattcataa atggttgtaa
     9121 aacatagaca ccaccattta ttctgtccaa atcccaatga cttcccatgc ctatatgtga
     9181 atttaatatc aacggtctat attgagttaa aacacctaca gtatcaatac cagagtttga
     9241 acaattactt aacacaaaat ctataattct atacttacct ccaaatgata cagctggttt
     9301 ggcaattctt tttgtaaaaa cacctaatct tgaaccttgc cctcctgcca aaatcatagc
     9361 taacatctct tttttcattt aatgacactc ccctcatcta gtaattaata tttataattg
     9421 catttttact tttataaact ttatcagtca ataaatgccg tgtataaatg ttgcttaaat
     9481 aataccttaa taaaaataaa ataatatcaa ctatacaatt gatataatgt ataccccata
     9541 atgaacttac cgacgtatac attattttcc ataagcacta tagtatttat taatattcaa
     9601 ttatcggtta aaaaataata caaattttat tttataaata taaaaacatt ttttataaat
     9661 cccttgtgtc atataaacat gcttattttt taatttgtta aattcgtaat agcaaaatat
     9721 aatctaatat ttgttaaata attatccaaa gaaaatcaag taattattaa tcaactttat
     9781 tttttaattg atacttcttt gtctataatt taaacaaaag gatataaact tttaaattat
     9841 ttattagttt tcttagtatt aatgttta
//
LOCUS       DAELXX010000129         9654 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.131, whole genome shotgun sequence.
ACCESSION   DAELXX010000129 DAELXX010000000
VERSION     DAELXX010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9654
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.131"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            940..2298
                     /locus_tag="KPH29_003182"
     CDS             940..2298
                     /locus_tag="KPH29_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBG1468754.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     ASGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
     gene            2404..3198
                     /locus_tag="KPH29_003183"
     CDS             2404..3198
                     /locus_tag="KPH29_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBG1468755.1"
                     /translation="MVTIKEYSIPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDEKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            3191..5497
                     /locus_tag="KPH29_003184"
     CDS             3191..5497
                     /locus_tag="KPH29_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG1468756.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVAESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRIIRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKLLSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPIFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            5636..6913
                     /locus_tag="KPH29_003185"
     CDS             5636..6913
                     /locus_tag="KPH29_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG1468757.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIESLKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASLNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            7096..7818
                     /gene="yqeC"
                     /locus_tag="KPH29_003186"
     CDS             7096..7818
                     /gene="yqeC"
                     /locus_tag="KPH29_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HBG1468758.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSFINYFANFYRDKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHKTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSSTVSIENLKNIVLNPNGLFKNYSGKRVLFINKVENEKYKNLAIKLIENI
                     KEYESEIEIFYGSVKQKFCVRY"
     gene            7925..8494
                     /gene="mocA"
                     /locus_tag="KPH29_003187"
     CDS             7925..8494
                     /gene="mocA"
                     /locus_tag="KPH29_003187"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HBG1468759.1"
                     /translation="MINAVIMASGFARRMGANKLLLNYKGCSIIEHVFREVSKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKMDYERIGGKL"
     gene            8491..9510
                     /locus_tag="KPH29_003188"
     CDS             8491..9510
                     /locus_tag="KPH29_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HBG1468760.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
BASE COUNT         3811 a         1008 c         1813 g         3022 t
ORIGIN      
        1 tgttgcagat acaaatatag taaaggagag tacattggta caactgagat acatagctta
       61 ttaagttttt ctgtcttatg agaattatat tataaaatat tcataaatat tttgagttta
      121 aattaaataa ttattttatg cttagattaa ttgatattta attaatttaa taacaaaaac
      181 agtgaaagag taaataatat tatttacagg gggtaaagca aagtatgtta aataaattgt
      241 acttttggcc ccgtacatag agaaatctat gtaggacaca actttaaaac cagtaaatcc
      301 taaagccttg caactacaat aaaagataga aagaaacttt tatgaatgtg gtgaaaacca
      361 gaaacaatag tgaggatggt atatggtgag agcctaagta cttaaaaatg gacatttggt
      421 cgccaagcct agaaatagga aggtcaaacg actatctcga gagagagtac actataagcg
      481 attgatagtg gaaacgggtt gctcttagca ggtaatgctg aagatgaaga aatagtctac
      541 gctcatatga aagtatgagg tcactagatt tgaagtgaca tctataaagt agcgtttata
      601 ggtaaatgtt taaaacttga aacatgtaaa tacttatgaa aacttataac ttataatgat
      661 taagattgaa agatttaaat tgaattaaat ttactataaa aaattaaaaa ttcatagcta
      721 taatactctt atatttgtaa agcatggtat ataatgtata caaaattaaa gaatacgtgt
      781 atttgttaat aattaaattt attgcatctg ttttttaaga ctaaaaactt aaatatatgt
      841 tggagtatag ttgatattta tgaacatcat taatttaata tattagattt ttaagtattt
      901 atataattca ataagtttta tgtaacggga gtttttatta tgaaagcaaa aagtacatca
      961 aaatttgaat tagacggaat accaccacta aaggaagcaa tacctttagg gttacaacac
     1021 ttaatggcaa tggtaatagg tagttgttta ccagcgatat tgattgcaaa tgcatctgga
     1081 cttaatcata agatgtcaac attattgatt caagccgcat tagtttttgc aggattatca
     1141 actttattac aattatatcc aatacctttg tttaaaggtt ttaaattagg aggaggaata
     1201 ccagttataa tgggagctac agctatgttt ataggagctg gggtatctgt tgcaagtaag
     1261 tatggactac cagtattatt tggctctcaa ataatagctg gtatagtaat aattataatt
     1321 ggttttggtc ttaaaaaaat acgatttata tttactccag ctgtatcagg aacaatagtt
     1381 gcatgtatgg gtcttggact ttttccaata gctataaaaa acttagctgg gggtatgggc
     1441 aatgagactt ttgggaatcc aattaatttt atagttggaa tatctgtatc tcttatgata
     1501 ttaggattaa ataagtatgg aaaaggatta tttaaagatg catcaatatt agttagcata
     1561 atttttggat acattttatc attaattttg ggtatagtaa atttttcttc aattcaggag
     1621 tttactttgg ttgcgcttcc aaaaccactt gcttttgggc tagatataag acttgaagtt
     1681 gttgtaatgt tttcaataat atatttagtt gagatagctg atataatggg tgcttgtaca
     1741 ctttctgcgg taggagggct taatagacaa gtaacagatg aagaactttc ttctgctgtt
     1801 ttaggaaatg gagtactctc tacaataggt gcattatttt ctgcaactcc aatggggatg
     1861 tttggacaaa attctgcaat cgtttccaat acaaaggttg taagtaaatt tgtactagct
     1921 attggaggaa ttggattatt tttggcagga atatcgcctt tattagctaa tttaataaga
     1981 acaattcctc cctgtgtggt tggaggagca acactcgtta tattttcaac tcttacaaca
     2041 tctggtttaa gacttgttag tatggatgga tttaatcaag aaaatagcat gatattaggt
     2101 ctttctatgg cttctggtat tggattcatg gtagctcctc aagttcttga aaaatttcca
     2161 aaatttatag agacattact tgcagattca agtgtagttt caggagcaat ggtagccata
     2221 ataattcaag ctttatatat ggtaaaattt cctggaaata aaagtgataa ggttgaggat
     2281 aaaaataaaa gtttataatg taaaggttta atatatgtga attaaatata ctacaattat
     2341 aaatataaat aaaagattta ttaaaaactt ttgtgtttta ataagttaat gatgggggat
     2401 aagatggtta ctataaagga atattcaatt ccacagagtt tagaagaagc atataaaatt
     2461 ttaatttcta aaaaaaataa tgttatttta ggtggatgtg gatttataaa attaagtaat
     2521 aaaaatatag gaacagcgat agatttaaaa gatataaatc taaattatat aaaagaggat
     2581 gaaaaaaata ttttgatagg tgcagatact tcacttagaa gtttagaaat tgataaaact
     2641 ataaaaaatt actgtagtgg aatactttca agcgctgttt ctaatatagt tggtgttcaa
     2701 tttagacaag gggctagaat tggtgcaagt gtattttcaa agtatggctt ttctgactta
     2761 ataccagcgc ttttggttgt tggagcgaag gtgagattat acaataaagg aatactagag
     2821 ttaagtgagt ttttggatag tgaattaagt agggatatat tggtcgaagt aatactacca
     2881 aaaaaagatg cgatagctgt ttttgactca ataagaaaat gcacaggaga tttttctgta
     2941 ttaaattcag caatgctaaa agaaaatgat acttacaaaa tagcaattgg ggcaagacct
     3001 caaaaagcta gattagcact tgaagcgagt aatatattaa ataaagaaaa agatatagat
     3061 aaggcatcta ttgttgctag taaggaactt acttatggaa gtaatatgag agctagtaaa
     3121 gagtacagaa aggatatggc aagtgcatta gttaaaagga tgtataatgc tattgaggag
     3181 ggaatgtata atgattaata aggggataag aaagatagat agtacagcaa ttgtcacagg
     3241 aaaacctcta tatactgaag acctcattat gcataaagat gttttgacat taaaactact
     3301 tagaagtcca catgctcatg ctaagataaa gaatatagat acatcaaaag ccgaaaaaat
     3361 tccaggggtt gtgggtattt atacgtataa agatgtacca caggtcagat attgtgtgcc
     3421 aggtcaaaca gaaccagaag catcacctta tgatagagtt ttacttgaag atacagttag
     3481 atttattgga gatgaagtag ctttagtggc tgctgaagat gagaaaacag cacttaaagc
     3541 cttaaaaatg ataaaggtag attatgaggt tttaaagcct gtgtttgatg caagagaagc
     3601 agaaggaaat gaagttgtta tacatccaga aaaagacata gtagagccat acccttttgg
     3661 attggatgca aaaaataata tagtatcaaa agaatttttt gaatttggag atgttgaagc
     3721 taagcttaaa gaaagtgatt ttgtggctga atcttcatac aaaacccaaa gtcaatcgca
     3781 ttgcatgatg gaaactcata ggtcatattc atataaagat ataaatgata gaattgttgt
     3841 agtttcatca ttacaatcac cacacaatgt aaagagaata attagaaaag tattgggtat
     3901 accatcttca aaaataagag taattaaacc tagaataggt ggtggatttg gaggaaagaa
     3961 tatagcagca acagatatat ttactgcatt tgttactatg aaaactggta gaccagcaaa
     4021 atttgtattt acaagaagtg aaacttttgt aggttctaat agtagacatg aaatgttttt
     4081 tgatgttaaa gttggtgcag ataaagaagg aaatttaaaa gcaatagagc taaagtcatt
     4141 aaacaataca ggtgcttatg gtgggaatgg tgtctctgtt tcttccgaat ctggacacaa
     4201 taccttacca atatataatg atattgatgc tgttagattt aatgctagaa cagtatatac
     4261 taataggtta ccagcaggag cacttagagg gtatggtgct acacagggaa tatttgcaat
     4321 agatagtgct attgatgaat tggcacataa gattggaatg gacccacttg aatttagaat
     4381 taaaaatata atagataggt ataccaatgg aaaagcagct gaagacgaaa tattaagttg
     4441 tgagctgaga caatgtatag aaaagggcaa gaaattaatc aattgggatg ataaatatcc
     4501 atgtaaagat ataggaaaca acaaagtaag agcagtaggt atggcagtag caaatcatgg
     4561 ttctggtata ccgcgtgtag atatggcaac agtcactata agaatggatg aagatggcac
     4621 atataaacta ttatctggtc ttgctgactt aggtcaaggg gctgatacaa ttcaagctca
     4681 aattgctgcg gaagctttaa atactactgt agatagaatt tctatttata caggtgattc
     4741 agatttatgt ccctatgata caggagcatt tgcttcttgt acaacaacgg ttacaggaaa
     4801 tgcaacaata aagacagcaa ataaattaaa agatatatta ctttcagttg cagagtcaaa
     4861 gctaggattt aaaaaagaag atttagagtt gggaaaagac agggtttttg ttaaaaacga
     4921 taatagcaag tttgttctat tagaggaatt gggtagagaa tctgttggag gaataggatt
     4981 tgctagtgat gatgtaccag tagttttgga agcaacagaa tcttttggca tgttgaaaaa
     5041 aataaaacca tttatagcag gatttgcaga gatagaatta gataaaaata caggcgaata
     5101 taaggtcata aattttgcat gtgtacctga ttgtggaaca gttataaatc caactttagc
     5161 tagaatacaa gtagaaggtg gtgtgatgat gggtataggg tttgcaatgt atgaagaccc
     5221 tatatttgat aaagatggga gattattaac agatagtttc ctacagtata aagttcctac
     5281 aaaaaaagaa gttggaaata tattggtaga ttttgctgag aatttcgacc caggaggtcc
     5341 ttatggtgct aaatcattag gagaaatagt tatacataca ccagcgccag ctatagcaaa
     5401 tgcaatattt aatgctacag gagttcgttt aagagagctt cctatgactt ttgaaaaagt
     5461 ttatatgggt atgaaaaata ataataaaca attataataa ataataaggt gtataaaagc
     5521 aagtaaattt aaattatttt gtaaaataaa gtaattaaat ggacattata tatggtgttt
     5581 tgtaaaaata ccgtatataa tgtctagtag agtatattct aaaggaggtg agttcatgtc
     5641 aaacagaata aaaaatatac actatggaac tcagagacaa caattaaaaa acttaactaa
     5701 aaaagaatat caagctctac gagagatgtg cttcttagct aagaacttat ataatgttgg
     5761 tacatataat gtaagacagc attatttcaa tcaaggagaa catcttaact atgaagctaa
     5821 ttatcatgta tgcaaagaaa atgagaacta taagcttctc aatagtaata tagcgcaaca
     5881 aatattaaaa gaagtagaca gttcgtttag gtcatttttt gggttaatta aattagccaa
     5941 agaagggaaa tatgacttta gagggataaa atcacctaaa tatttagata aagaaggatt
     6001 ttttagcata ataataggtc aaataagaat caaagaagat gggattttaa atgtacctat
     6061 gtctccagca tttaaaaaac tatatgggaa agtaagtata aaagttccac aaaatatact
     6121 agagaaaaaa attaaagaaa taagaataat acctaaacat aaagctaggt tctttgaaat
     6181 tcagtattgt tatgaaatac cgaaatcaga ggaagtttca aatcaaaaaa atgcactagc
     6241 tatagacttg ggtttagaaa atttatgtac atgtgttacc aatttaggtg atagttttat
     6301 catagatgga aaaaggctaa aatctgtgaa tcagtgggca aacaaacaga atgataaaat
     6361 agaatcctta aaattaaaaa agaatataac tgcaataaca agaaaacaat ttaaagtttg
     6421 gaataagaga aataatcaag ttaaagacta tgtaaataaa acctgttttt atataattga
     6481 ttattgtgta aaaaataata ttggaaattt aattgtagga tataatgcta agttagaaaa
     6541 aagtgataat atggagagaa aaatatatag aaattttata agaatcccat ttggagagat
     6601 aaaaggaaaa ttagaatatc tatgtaaaat aaaaaatatc aattttatta ggcaagaaga
     6661 aagctatact agtaagtctg atttttttgc tgatgatttt ctacctgata tcaatgtgga
     6721 caatgttaaa aaatatgact ttaaagggga aagaataaca cgtggacagt acaaatccag
     6781 tgttggaatc attataaatg cagatgtaaa tgcgagtctt aatatactaa aaaaatcaca
     6841 agtagttaat ttatctaatt tacagaataa tcctcaaata ttaaagcaac cacagagagt
     6901 aagaatatct taattatatt taaaagataa actgatagcg taagcttaca gcttagcggc
     6961 gaattggatc cgccttgttg tttacaacag aatctattta ctttcgagtt tatggaattt
     7021 caaaaatcac ttttactaaa aaggtctaac tataataatt aagagattag aaaactggtt
     7081 ataagaggtg aaagtgtgga actatcaaat ataatagaaa agaatgaaat aataactgta
     7141 gttggagcag gaggaaaaac ttcgtttata aactattttg caaattttta tagagataaa
     7201 ttgaaagttc ttcttacaac aacaactaaa atatatgtac caaacgatta tgacaatata
     7261 attattacaa tagatggaac tgttattcca tctatttgtc atggaataac agtctgtggc
     7321 agttatataa ataatgaaaa taaattagta agtattgatt cctctatttt ggatgaaatt
     7381 gtagaccaat ttgatttagt tttaatagag ggagatggct caaagagaaa aaaacttaaa
     7441 ggttggaatg ctaaagaacc agtagtatat cataaaacta caaaaaccat agggatatta
     7501 gatataacat catttggaat gaatattaat gaagagaata tacatagagt agaaatattt
     7561 aaaaaaatag caaatttaga tacaagtagt attaatagta gttctacagt aagtattgaa
     7621 aacttaaaaa atatagtatt aaatccaaat ggattattta aaaattattc tggaaaaaga
     7681 gtattattta ttaataaagt tgaaaatgaa aaatataaaa atttagctat aaaattaata
     7741 gaaaatatta aagaatatga aagtgaaata gagatatttt atggaagcgt aaaacaaaaa
     7801 ttttgtgtaa gatattgaag tattaaaata gtttatatga tattatatat atagataata
     7861 tcaatagaga tattataaaa atcatagaac tatcaatata ttataaatac caaggatgat
     7921 atatatgata aatgcagtga taatggcatc aggctttgcc agaagaatgg gagcaaataa
     7981 acttttatta aattataaag gctgttctat aatagaacat gtatttagag aagttagcaa
     8041 gattgatttt catcaagttg tagtagtaag tcaatatgaa gaggttttgt tattgtgtaa
     8101 taaatatggt tttgaatata tagataataa aaatgcaaat ataggtcaaa gtgaatctat
     8161 aaaattagga gttttaaata gctcaaaatg tgatggttat atgttctttg taggagacca
     8221 gccatttatt gataacttat atatagataa aattataaat acttttaaat tagatagaga
     8281 ttttatagta attccaagat acaaagatat gtgtggaaat ccagtcatat tcccatatag
     8341 taagaaagaa gaattgttgg cacttaaaga agatgaaaaa ggtaaaacca ttttaaaaaa
     8401 ttcatcaaaa ataaagtatg tagaagttcc taaagaaatg ttatttgata tcgatacaaa
     8461 aatggactat gagagaatcg ggggtaaatt atgaagctaa taaaaactat agatgctgtt
     8521 ggacaagttt tatgtcacga cataactcaa ataatacctg gagaatttaa aggaagaaaa
     8581 tttaaaaagg gtcacatagt aaaagaagaa gatattccag tgttattatc tcttggtaaa
     8641 gataatttgt atgtatggga aaagtcagaa ggaatggttc atgaaaatga aggcgcattg
     8701 tttttaaaag aactaactgc tggagaaaat ttagatttta gtgaaataaa agaaggaaaa
     8761 atagatttta tagcagcatg tgatggtcta ttaaagatag atgttgatgc actttttgat
     8821 ttgaattgtg ttggagaaat aatgatggct actcttcata ataattttat agtaaataaa
     8881 ggtttaaaag tagctggaac aagagtgata cctttggtta tagatgagaa aaaattagaa
     8941 gatgcaaaaa aagtagttgg taatagaaaa atagtaaatg tagtaccttt taaacctaag
     9001 aaagtaggaa ttgttactac aggaaatgaa gtgttttata gcagaattgt tgataaattt
     9061 ggaccagtaa tagaagaaaa agtaaaaggc tttggttgtg aagttatagg acagactata
     9121 tgcccagatg ataaagaaat tataaagagt gcaataaaag aatttataag tcaaggagca
     9181 gaacttatct gttgtactgg cgggatgagt gtagacccag atgatgttac gccaacagct
     9241 ataaaagaaa ctggagctga tttagtgaca tatggttcac caatattacc aggagctatg
     9301 tttttacttg cttattatgg agaagttcca attatgggta taccaggatg cgcaatgtat
     9361 cataaaacta cagtatttga tatagtttta tctagggttt taattgatga aaagcttgat
     9421 aagtatgata tagctagata tggtcatggt ggcctttgta tgaactgtga tgtatgtact
     9481 tatcctgcat gtaattttgc taaaatataa agttattaaa aaagagtatt tacaaattaa
     9541 gtactctttt taaatatgaa acaaaacagc tagaatgatg ttttaagaaa agcatattta
     9601 tttagaaatt ataataactt attttcaatg tgtaaaagaa tgaataaagt tgca
//
LOCUS       DAELXX010000130         9649 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.132, whole genome shotgun sequence.
ACCESSION   DAELXX010000130 DAELXX010000000
VERSION     DAELXX010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9649
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.132"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(296..880)
                     /locus_tag="KPH29_003189"
     CDS             complement(296..880)
                     /locus_tag="KPH29_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBG1468761.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKDLVRDYNQTDSSDLEK
                     KEKILNELLGGKDKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNVKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
     gene            complement(1202..3046)
                     /locus_tag="KPH29_003190"
     CDS             complement(1202..3046)
                     /locus_tag="KPH29_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBG1468762.1"
                     /translation="MQKRILRNFIFTGKNILSKLVFIIKYNFSTLILFELFHKGLALF
                     FIWPSIRYIFDILIKSLHIVYLNMDNFIKVLTNPMSIVLIILSVIILAFYAFFEFTSV
                     IICFNKSIKYEKIGLFELFKISFKNSIKLLYPKNILLFIFVILIIPLVNTVLISGFIG
                     KIQIPEYILDYIKSDIILNIIYMIFLLIIYISVIRWIFSIHEITLNTENFKRARKESV
                     NLIKRKIIRTIIYSLILSLVVAIIGYAIYHSGIIFIGIWTKYCTDMEFLKNVFINRCI
                     AFEEYFRLILSIFIFILDIGFISATYYQYKGVNISKNNIKKEKKSISKIVFNLVLILL
                     ISYIESVAFSSHINGMFNTSFSFYTTAIAHRAGAEMAPENTLAAIEEASNAKADYAEI
                     DVQETKDGELIVLHDSNFKRTTGVDKNVWDVNYNEVKTYDAGSFYYYGKGYYDEKFVG
                     EKIPTLKEMIKYSKGKVKLLIEIKLNGHEKGDIVKKVVQLIKENNFENQCIVASMDKT
                     ILQKVKKLDENLLTCYFMAIAYGDFSKLNYVDIYGIESTFVNKTVVEKIHKMNKKIFV
                     WTVNSDSLIEKMLDLNVDSIITDNPYLIESAIYWKKNGFISQVSDYLF"
     gene            complement(3341..4303)
                     /locus_tag="KPH29_003191"
     CDS             complement(3341..4303)
                     /locus_tag="KPH29_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBG1468763.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLEA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIVLTLNDSGLKYLSTDLYEEL"
     gene            4494..5045
                     /locus_tag="KPH29_003192"
     CDS             4494..5045
                     /locus_tag="KPH29_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1468764.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIINYYPV"
     gene            complement(5583..5870)
                     /locus_tag="KPH29_003193"
     CDS             complement(5583..5870)
                     /locus_tag="KPH29_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468765.1"
                     /translation="MKKILYALYSFIVIIANFRLKEKNYNFILLAIFFILLLIQNGVL
                     KTGYQNRATFYQDNYFNGEDKNDKIKFITIKIFIFLSLPLCFNILFNYLTS"
     repeat_region   6279..7233
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=6478..6506
                     /rpt_unit_seq="atttacataccacatagttaatataaaac"
     gene            complement(7429..7668)
                     /locus_tag="KPH29_003194"
     CDS             complement(7429..7668)
                     /locus_tag="KPH29_003194"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468766.1"
                     /translation="MECYLKRTLIIFSKAFLSISFKTRKYLFIVIFILVCPNLSEMIF
                     ILVPARRRIIRIDNIVSIYKYVGVLKLINYSILIL"
     gene            7880..9151
                     /locus_tag="KPH29_003195"
     CDS             7880..9151
                     /locus_tag="KPH29_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG1468767.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIILEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSIADTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPM
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSEAFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKLIEEPNI"
BASE COUNT         3571 a         1396 c         1028 g         3654 t
ORIGIN      
        1 tattatagta ttttattctt acttaatcat catactatag caaaatacta acctaaaagt
       61 tagtatttta cttaaaaata agtgcttatt caaacatcaa atagaatata tactattata
      121 aataaatcta taaaggaatg acttttataa aaaatggtgg aaatgtaaga tagaagtgat
      181 aaaaactaca ttccacaaaa atttaacaaa tgaatatgga tgtgaaagta ttggaaaata
      241 ccctatgtat aaagtaagaa aggtgttttt tgagattttg caagaaaatt taactttatt
      301 tgttttctcg aataatacgg catggatttc cgtatgcaat catattactt ggaatatctt
      361 ttgtaacaat acttcctgca cctataacta cattatctcc aatagtaaca cctggcatga
      421 taatagcacc cccgccaatc caaacattat ctccaatagt aactggagct gtttgagttt
      481 tacaaaattc aaatgaacca tcttctttga cattaccaaa tctatcacca gcattagttg
      541 gatgaaatgc agtatatatt tgtacgtttg gtgcgattag tgcattatcc ccaatgataa
      601 ttttattatc atctaaaaac gtacaattca tattgacttc acaattattt ccaaaataaa
      661 tattatttcc ataatctaca taatacggtg cagtaatcca taagttttta tccttaccac
      721 caagtaattc atttaatatt ttttcttttt tttccaaatc cgaggaatct gtttggttat
      781 aatctcttac taggtctttt gctttatgcc attgagttaa caactcaata tcaccacaat
      841 cgtaaagttc tcctgccaac attttttctc gctctgtcat gtttttaatc ctccaaattt
      901 atattgtttt tcatatatat tctagcataa gatttattaa tttatatatt ttacttatat
      961 aaaatttaat aaataacaag atttattaaa tatacagttg acaatctatc tctctgtcat
     1021 cttaaaactc ttttaactgt aataatctca gagcaatttt ttcactttta ttccttgttt
     1081 atagctgata agcacaattt ttataagaat aaccttaatt taacaacctt atttatgtta
     1141 aaaagagata ttattgattt taataatatc tcttttactg gataatacaa ttatagaata
     1201 tttaaaataa ataatctgac acttgactaa taaatccatt tttcttccaa taaattgcac
     1261 tctctataag gtatggatta tctgtaataa tactatctac atttaggtca agcattttct
     1321 caattaaact gtcactatta acagtccaaa cgaagatttt tttgttcatt ttatgaatct
     1381 tttctactac tgttttatta acaaaagttg attcaattcc atatatatca acataattta
     1441 actttgaaaa atcaccatat gcaattgcca taaaatagca tgtcagtaaa ttctcatcca
     1501 gtttttttac tttttgtaat atagttttat ccatagatgc aactatacat tgattttcaa
     1561 aattattttc tttaattaac tgaacaactt tttttactat gtctcccttt tcatgtccat
     1621 taagttttat ttctattaat aatttcacct tacctttaga atattttatc atttctttga
     1681 gtgttggtat cttttcccca acaaactttt catcataata accttttcca taataataaa
     1741 agcttcccgc atcatatgtc tttacttcat tataatttac atcccaaaca tttttatcta
     1801 ctcctgtagt tctcttaaag tttgaatcat gaagtacaat aagttctcca tctttagttt
     1861 cttgaacatc tatttctgca taatcagctt tagcatttga agcttcttct attgctgcca
     1921 aagtattctc tggagccatt tcagctcctg ctctatgagc aattgctgtt gtataaaatg
     1981 aaaatgacgt attaaacatg ccatttatat gtgaagaaaa tgcaactgac tctatataag
     2041 aaatcaataa tattaatact aaattaaaaa caatttttga tatagatttt ttctcttttt
     2101 taatattatt tttagaaata tttactcctt tatattgata ataagttgct gagataaatc
     2161 caatgtccaa tataaaaatg aatattgata aaatcaatct aaaatattct tcaaatgcaa
     2221 tacatctatt tataaataca ttcttcaaaa attccatatc agtacaatat ttagtccata
     2281 tgcctataaa tataattcca ctatgataaa tcgcatatcc aataatagct actactaaag
     2341 ataatattaa tgaataaata attgttctta taatctttct ctttataaga ttaacacttt
     2401 cttttcttgc tcttttaaaa ttttctgtat ttaaagttat ctcatgaatg ctaaaaatcc
     2461 atctaataac cgaaatatag attattaaaa gaaaaatcat atagattata tttaaaatta
     2521 tatcagactt tatataatct aatatgtact ctggtatttg tatttttcct ataaaccctg
     2581 aaattaaaac tgtatttact aatggaatta ttaatatcac aaatataaat aatagtatat
     2641 ttttaggata taacaatttt atagagttct taaatgatat tttaaagagt tcaaataacc
     2701 ctattttttc atattttatc gatttattaa aacaaataat tacagaggta aattcaaaaa
     2761 atgcataaaa agctagtatt ataactgata gtattatcag aactattgac atgggattgg
     2821 tcaaaacttt tataaaatta tccatattta ggtagactat atgtaaactt tttattaaaa
     2881 tatcaaatat atatctaata gatggccata taaaaaataa ggctaatcct ttatgaaata
     2941 attcaaataa aattaatgta ctaaaattat attttattat aaaaacaagt ttacttaaaa
     3001 tatttttacc cgtaaatata aaatttctta aaattctctt ttgcatgttt atctccttgt
     3061 atctgtttgt tctaatttta tcatatgttt taaatacatt tagaacatta atatgtatta
     3121 ttccaagtta tgatgtataa atgcaaaaac acatactctt ttttattgca tagctaatca
     3181 tcaatatcta tatagcttaa tacttataag taggatactc catatttaca aaataaaaaa
     3241 gtatctcttt tataactttc aaaaatcaca ttgagatact ttttataatt taaatctata
     3301 tacttaattt aaaaaacatt gcataactat tgtgctatta ttacaattct tcatataaat
     3361 cagtacttaa atattttaaa ccactatcat taagagttaa aactatattt tttccttttt
     3421 cactgctctt taacaactta attgctgcac acacatttgc ccctgaagaa aatcctgcaa
     3481 atacaccttc taatttagct aattttcttg ctacatcagt tgcctcctcg tctgtaactt
     3541 gaatataacc atcaataagt ttcttgtcta ctaatggcaa atccatagaa tatcctccac
     3601 cttgtatttt atgcccttgg tcagttattt ctttacctga ataaattgct gctgtttcag
     3661 gttcaacaac ataacatttg atagaagaat tatgtttttt aagtgttttt gaacatcctg
     3721 caaaagtact accacttcca agaaaatcta caaatacatc aatatttcca tccaattgtt
     3781 cccacatttc ttctgctgta tacttttcat gtgcatgatt acaagcttct aaattaaatt
     3841 ggtctgctct aaatgcattt ctttcttttg ctattttttg agcctcaatt tctacaagtg
     3901 ccaaatcttc accagatacc tgtccgtgaa ctgaattagg catttggtct acaagaacta
     3961 cttcagcacc taaagctttc atcattcttg ctctttccat agaatttcct tttgacatac
     4021 acgctataaa tttatatcct ttacatgcac aagctattgc tagtcctgtt ccagtattac
     4081 cacttgttaa ttccacaact gcttgacctg gaactaataa acctgcattt tctgcttcgg
     4141 taatcatttg taatgctaca cggtcctttt tactaaatcc cggatttaag tattccatct
     4201 ttgcataaat attaccttga actccaaatt catctgctat tttggataaa tgtaacatat
     4261 gagtattgcc tatagcctct gtaacattat taagtacacg cattacaatt cccccttaat
     4321 ctctcaacta tcatccgttg aaaaacaact ctttatgcag tataatttat attacggatg
     4381 tacagtgtat ttaacgttca ttatattata catttgtaaa ttataaataa tgttaaataa
     4441 attatacatt cgtaaattat atttgtcaat aatttacatt ttaaggagtt actatgagta
     4501 ttaatactgt tattgctaaa aatttaaata gattacgtaa tgaacgtaac ttaagtctag
     4561 gtcaacttgc tgaattatct ggcgtaagta aagtaatgct ttcgcaaatt gaaaagggag
     4621 actcaaaccc aacagtaaac acaatttgga aaattgcaag tggactaaat gttccatata
     4681 ctgcaatact ggaacaacct caaaatgaaa cttttattgt atcaaaaact gatatagatg
     4741 tacaagtttc agaaaataaa gattatcgtc tttattgtta ttatcccaat acaccaacta
     4801 gaaacttcga attatttcaa atggaattag aagaaggtca tagttataca tctgttggtc
     4861 attctgaaaa atcacaggaa tatattatga ttatagaagg tcaattaaaa ttagaagtaa
     4921 atgactctat ttatcaactt agagaaaatg actctatttg tttttcagct gaatcaattc
     4981 atacatacca taatcaagga gaaaaaacat tgaaagcagt aataataaat tattatccag
     5041 tttagccatg ccatttaata atagaataat tattttctat acaaaaagat aaatcctttg
     5101 attaggattt atctttttta ttttattttt ctttataaat tcattatata gctttacact
     5161 tttatcaatt atgttataat aagcttatat aaaagtttta tatttttgac aaatggagta
     5221 caaaatactc ctttttcttt tttattattg tgataattat ttagttagta ttaaaatatt
     5281 atcacttaaa acaaatacaa aacgtctctt atatgtacat ttttcattgt tgtaataatg
     5341 atttgtgtat gtatttctca caattggagt gcttatgcac tctttttaaa tacctttatt
     5401 accataacct aaaagctatt ttattatccc ttttagttta tttttaagta gttaaataaa
     5461 ttattttata ttttttcttc atttatatga aacttttgtt ttatcactat atgcacttta
     5521 tatttaatat aaacaacaca taatcttact tttatcataa cactcctata tctaaatata
     5581 atttaagaag ttaaataatt gaatagtata ttaaaacata atggtaatga caaaaatata
     5641 aaaatcttta tagttataaa ttttatctta tcatttttat cctctccatt aaaataatta
     5701 tcttgataaa atgtagctct attttgataa cctgttttta aaactccatt ttgaattaac
     5761 agcagtataa aaaagattgc taataaaata aagttataat ttttttcttt taatctaaaa
     5821 ttagctataa taacaatgaa actatataaa gcatataaaa tttttttcat aaaaaacacc
     5881 taaattctat atttgtattt tatattgagc caaatctaat ttctgtatat ttattaagtg
     5941 cagttctagt tcctattgta gtagcattaa atatttttta atttttattt ttaaaggaca
     6001 tctctttata tttaaattat agtatgaata ttcataaatt tcaatctatt tataagataa
     6061 ttatttttta tatctaatat cttgtcatta agttttatta tatatattta taaacttttc
     6121 aatacttttt ataattattc tacttttata ttttaatcat taaaactaaa gtcaatgtta
     6181 tctggtaatt atttactaca aatgataaaa aataattata ttttaaagta gaaaatgctg
     6241 tataaattat tggggtattt tacctatact tgttgcaaat ttacatacca cgtagttaat
     6301 ataaaacaaa tttcaagcga ttggacaacg aataaaaatt gcaatttata tatcatgtag
     6361 ttaatataaa gcatatttgt gtcgatatat agttctaact aagtataaga tttacatacc
     6421 tcatagttaa tataaaacaa tgtgtccgaa ttggacacat agaaaggaaa agtaaaaatt
     6481 tacataccac atagttaata taaaactatg cttatatatt atgtttattc tgttgtttct
     6541 aaaatttaca taccacatag ttaatataaa acgatttttt catgcggagg aaaattgatg
     6601 tggggacata tttacatacc acatagttaa tataaaacag ataatctagg cacacataca
     6661 atgcgcaaaa ctttatttac ataccacata gttaatataa aacattcaga cgatataggc
     6721 gcaggagcta agtattctga aatttacata ccacatagtt aatataaaac cagcacgcca
     6781 agtataagta aaacttccac tatcaccatt tacataccac atagttaata taaaactaaa
     6841 tgttgctccg taacctaaaa acataatgct ccaatttaca taccacatag ttaatataaa
     6901 acgtgaaaga aaaattaata tgctatttac attttacaaa tttacatacc acatagttaa
     6961 tataaaacca ttccacaatt tatacaattt tttctgttct aaaacattta cataccacat
     7021 agttaatata aaactcttct tatcttccag ttgcaaaatc tatgcttgac ctatttacat
     7081 accacatagt taatataaaa ctagtttgag tggtaaaagt acgaagtacc cctctttaat
     7141 ttacatacca catagttaat ataaaacata tgtaattcac cgttttaaat tatagataaa
     7201 ttaaatttac ataccacata gttaatataa aaccccaaaa taaactaagt attttcagta
     7261 cttacacaca tacaactctc tcaaatttgc agtgaaccat gagtagtgca attgataaca
     7321 tttatcatac accctcaatc ccttacattg caactgttaa accctacttt atcaaaaata
     7381 tcgctcactg caaaatttat atatttttat tatatcataa aattcaaact ataaaattaa
     7441 aatggaataa tttatcaatt taagaacacc tacatatttg taaatactta ctatattatc
     7501 tattctaata attcttcttc tagctggaac taatataaaa atcatctctg aaagattagg
     7561 acatactaat ataaaaataa ctatgaatag atattttctt gttttaaacg atatggataa
     7621 aaaagctttt gaaaatataa tcaaagttct ctttaaataa cattccattt tatgtaagta
     7681 agcattatat ttttcttaaa tgtcacttaa gtgtcagtta tatataataa tagtaaacaa
     7741 ctgtatttca ctaatatttg ctattatcta atagtattta tagtttagat ttgatataat
     7801 acatgtggta aaattttgat ttgtaaaaca aaattttacc tacatatatt aaaacaaatt
     7861 taaaactaag gagacctata tgttaaaagt attaaaagga aatataattt ttacaaaaac
     7921 ttcagaaact ttcacagtat ttgaaaatag ttatattata ctggaaaaag gtaaagtaaa
     7981 gggaatattt aaagagatac ctaaggaata caaaaaccta aaagtagtag attacactga
     8041 taagcttata atacctggta tgaatgacct ccatgcccat gcctctcaat ttaaaaatct
     8101 tggaatggct atggataaag agcttttgcc atggcttgaa acttatacat ttccagaaga
     8161 gtcaaattat aaagatatag aatatgcaac aaaaatgtat aaaaaatttg taaaagaact
     8221 ttggaaatct ggaactacaa gaatagcagt gtttgcaaca gtacataaag aagcctctat
     8281 gaaacttatg gatatattca aagaagttgg tcttggagca tacgttggta aagtaaatat
     8341 gaatatgaat tgccctgata ctttattaga aaacactcag gattctattg ctgatacaga
     8401 agaaattata aataagtata gtgatacaga ttctattgta aaaccaataa taacacctag
     8461 atttatacca agctgtacaa gtgaactttt aaaaggttta ggagatttat gtttaaaata
     8521 taatattcct atgcaatcac acttatctga aaattatgat gagattgaat gggttagaag
     8581 tctagaacct gaatctaaat tctatggaga tgcatataat agatataatt tatttggtca
     8641 aactcctact ttaatggctc attgtgttca ttgttctcag gatgaaatag atttaatgag
     8701 agaaaacaat gttatggcag ttcactgtcc agcttctaac tttaatgttg gtagtggtgc
     8761 tatgcctatc agaaaattaa tcgataataa tattagatta gcacttggat cagatataag
     8821 cggtggacat acattgtcaa tatttaaagc aatggtatct gcaatacaat tatctaaatt
     8881 atattgggtt aattctggta agaagtataa tttcttatct ttatctgagg ctttttatat
     8941 tgcaaccaaa agtggtggaa gtttctttgg aaaagttggt agctttgaag aaggttatga
     9001 ttttgatgca ttgattattg atgattcaaa tttaaatcat gacaattatt ctatacttga
     9061 aagacttgaa agatttatct atgttggtga tgatagaaat atcattcata gatatgtatg
     9121 tggaaaacta attgaagaac ctaatatata attataataa aaattttaat tatatattat
     9181 ggttaattta tgcaactata attaataaaa gagtatattt taaatctaaa agctataggt
     9241 tgaaacatac tctttttatt ttgtttattt atagctttag ttattaatca tatataaatc
     9301 tacaaaacgt attcctagta acttatattt tttgatatct acaatcataa gactatatta
     9361 ttggattcat acctcaaaaa tattgccttc tgaacattat tatattcaaa gttatgttaa
     9421 tttacaatga tatatctaac atgtattgat taaaaaaaat taaaacttgc ttttcatctc
     9481 ctatatctaa agaagtggca tttagaataa actattgtaa tattaaagtt gaaattttct
     9541 agtttattta ctctatagca ccatatttta atactattaa gtaaaaatgt agttaaaata
     9601 tatactaata tccctgaaat aagtatttct tttttattta gcacactta
//
LOCUS       DAELXX010000131         9514 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.133, whole genome shotgun sequence.
ACCESSION   DAELXX010000131 DAELXX010000000
VERSION     DAELXX010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9514
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.133"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(605..3712)
                     /locus_tag="KPH29_003196"
     CDS             complement(605..3712)
                     /locus_tag="KPH29_003196"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBG1468768.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     misc_binding    complement(3805..4037)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4212..4739)
                     /locus_tag="KPH29_003197"
     CDS             complement(4212..4739)
                     /locus_tag="KPH29_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBG1468769.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(4774..5559)
                     /locus_tag="KPH29_003198"
     CDS             complement(4774..5559)
                     /locus_tag="KPH29_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBG1468770.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            complement(5569..5826)
                     /locus_tag="KPH29_003199"
     CDS             complement(5569..5826)
                     /locus_tag="KPH29_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBG1468771.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFNILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            complement(5845..6303)
                     /locus_tag="KPH29_003200"
     CDS             complement(5845..6303)
                     /locus_tag="KPH29_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBG1468772.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(6343..7047)
                     /locus_tag="KPH29_003201"
     CDS             complement(6343..7047)
                     /locus_tag="KPH29_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBG1468773.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            complement(7057..7692)
                     /locus_tag="KPH29_003202"
     CDS             complement(7057..7692)
                     /locus_tag="KPH29_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468774.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVDTLVLKNEKVDAK
                     IPVKGTIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            8073..9113
                     /locus_tag="KPH29_003203"
     CDS             8073..9113
                     /locus_tag="KPH29_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG1468775.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGIITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
BASE COUNT         2871 a         1664 c         1079 g         3900 t
ORIGIN      
        1 agtttctcct attcggagtt tacctgctga ttgcccattt taaagtactt agggtttaac
       61 cttataccat ctaaccaatt ttttctactt tcgtaacttt cacacttagg tttatttcat
      121 ccttatgttt tagtttggtt agctttaggg gtttccagca attcaagtag tattggatag
      181 gttctatcct atctctacat ataggtttcc ctatatgctg actattttag ctactaactc
      241 atgactaaat tcacgagtgt gcgtagctat tttttaatca atacttaact ttttgttttc
      301 accatacatc aaaacgcacc cctttgcata ataaataaac caccctgcag aacacacgat
      361 aaatttataa ataaatttat cagaagtagc ttactaaaaa tattattcat cctcacttaa
      421 acgtttattc attaatattc tatcacatga cataaataat ttatatgatt tatatagatt
      481 tatttattgt aaattttgga atattaaaac attttattga atagataatt atattacata
      541 aaaaatccct atagaaatct ataaactaat ttctataggg attttggtta actaactatt
      601 ctttttattt ttttataact tttatacctg tcatatggtc atttaagttt tgttcctcta
      661 aagatttatc atctactctg tttatttcta aagctaaagt ttcagacttg atatattcat
      721 taaaatggtc aacagcctta gcaatttcat catcaccaca gtattctatt actatattat
      781 ctaatacttc aaagttatta gattttctta attgctgtac tttagatata aattctctag
      841 cataaccttc attaatcaac tcttcagtta gcttagtatc tagtataaca aaaagattgt
      901 tttcaacaga tacattaaat ccttccttag cagttatacc tattaatact aaatccttat
      961 taaattcaaa agcttcgcca tctaaatcaa ctgttaaagt ctcacctttt tcaagtttat
     1021 tagcagtttc atgagcattt aatttactta atgctccaac gaataaattc atcttagaac
     1081 ctaataatgg ccctgcttcc ttaaagtttg gttttaaact aaagttcata tactcatcta
     1141 aatccttagc aaatattacc tctttaacat taagctcttc ttttattaaa tcaactacat
     1201 cacttattgt atcttcaaac ttaccatcta ctaacacttt ttgtaatggt tgacgaactt
     1261 ttattctttc tacttctctt gaagctctac ctaaagtaac taagttttta gctaaatcca
     1321 ttttttcttc taatttagta tctactaact cttcattaca tttaggatat tcagctaaat
     1381 gaacagatac ttctcctgtt aagtttctat acatttcttc tgacatatat ggtgcaaatg
     1441 gagctatagc tctacaaaga ccatgtaaca attcataagt tgtattgtac actgcttttt
     1501 tatcttctgt taattcagtt gcccagaatc ttcttcttga acgtcttata taccagttag
     1561 ataaatcatc atttataaaa tcttgtatca tccttaaagt tttatttaac tcaaatatag
     1621 ctaaattttc ttctacatct ttcattaaat tattgaactt agataatatc catctatcaa
     1681 gttctggtct atctttatat tctacaaaaa attcagttgg atttatatca tctgtgtttg
     1741 catataaagt aaagaagtta tatacattct tcatagtacc tataaattta ctttggattt
     1801 cttttaatcc atccatatca aatttagttg gagtccatgg tggagaaaca tatagtaaat
     1861 accatcttaa tgcatctgct ccatattggt caaacaattc cattggattt actgtatttc
     1921 ctcttgattt actcattttc ttaccatctt tatcaagtac aaggtctgga actaaaacac
     1981 tcttatatgg tgcttttcct aatacaaatg ttgatactgc aagtaatgag tagaaccaac
     2041 ctctagtctg gtctatacct tctgatatat agtcagctgg gaacaattgg tcaaaatttt
     2101 ccttattttc aaatggatag tggtgttgag caaatggcat agccccacta tcaaaccaac
     2161 agtctataac ctctgttact cttgtcattg gttttccaca tttttcacat tttaagtgta
     2221 tatcatctac atatggtcta tgaagttcaa ctgtttctgg atttacatct tctatagctt
     2281 tttcagctaa ttcagctctt gaaccaactg aatctgtatg accacattca catctccata
     2341 taggaagtgg agttccccaa tatctagttc ttgatattgc ccagtcattt agattttcta
     2401 accagtttcc aaatctcttc tctcctacaa aacttgggaa ccattttaca ccattattat
     2461 tttctattaa tttatcttta agtttagtca tctctatata ccagcttggt tttgcatagt
     2521 atactaatgg agtttgacat ctccagcagt gtgggtaatt atgaactact ttttctttgc
     2581 tatataattt attttcagaa gctagccatt ttattatttc aacatctagc ccatcttcca
     2641 ttacaaatct accttcccaa ggagtagatg taaatttacc agtttcatct actggttgaa
     2701 taactggtaa atcatatctt ttacctgtgt tgtagtcatc ttcaccaaat gctggtgctg
     2761 tatgaactat accagttcca tcttcagtag ttacataatc tcctactgta ataaaaaatg
     2821 cttttttgtc aacttctaca aaaggcatta attgttcata ttctaaacct tctaggtctt
     2881 tacctttcat ttcttctaaa acttcataat cttcacctag tactttatca gctaaaggtt
     2941 tagatacata atatacctca tcaccttttt taacttttaa ataatctaca tcagcattta
     3001 ctgttaatga aacatttgat ggtaaagtcc atggagttgt tgtccatgct aagaagtatt
     3061 catcagcatc ttttttctta aatttagcta taactgtatt agtttttact tctttatatc
     3121 cttgtgcaac ctcatgacta gcaagaccag ttccacatct tgggcaataa ggaagtattt
     3181 tatgcccttc atatatatat ccttctttgt tgaatttgtt tagtatccac catacagact
     3241 ctatatagtt attatctaaa gttatatatg gattatctaa atcaacttca taagccattc
     3301 tctcagtcat tattctccat tgtttttcaa atgagaaaac tgactctcta catttttggt
     3361 taaattcagc tattccatat tcttctattt gttgcttgct agtaagacct aactctttct
     3421 ctacttgtat ttcaactggt aatccatgtg tatcccaacc agcttttctc tttacttgat
     3481 aaccagacat tgttttatat ctacaaactg aatctttaag cgttctagat aaaacatggt
     3541 gtactccagg atttccatta gctgttggag gtccttcata gaatacgaaa cttgggtcat
     3601 ctttcccttt ttctaaagtt ttttcaagta tatttatgtc tttccaataa tcaaaaactt
     3661 gtgcttctgc ttgttttaca gaactgtcta ccaatggctt aaatttcgcc atgtaaatca
     3721 ccctttccta ctaaatatta taattccaaa attttttatt ctttcacata aatttaaata
     3781 ctaaaaaact cgcccccttt aaaccaaagg gacgagttat aaatcgcgtt accaccctaa
     3841 ttctatatgt aaactaaata ctagctacat ataacactct aatagtataa cggtactaac
     3901 cggcacagct tagtagaatt taatcgttca gcctgcagct caagagtgat ttttatcttt
     3961 ataaattcat tggttctcag catacccaat tctctgtaaa attatctaaa gataaatgtc
     4021 tctatcaacg cttttaaaaa tttatctgtg tttatatgaa attttataac aaataatttg
     4081 aatagtcaat atatttttga aaaatataat aatttctatt tttcttacat ttttatccta
     4141 tttttaatct ttgttataga tttctcaaac tcacacagtt attctacata aaaaataaac
     4201 aaattacaag cttattctaa agttgtagca gcttcatcat tactataatt atacactgtt
     4261 gttccttcaa atgcatttac acatttttca tctacatcat aaaaaatctc atcaacactt
     4321 cttatttcat cttctaacag tgatttaaat ttatttctaa atatcttaaa ttctttgact
     4381 aaagcatcat actcttttct tatctctatg acacggttat tagcttgttc tataatttgt
     4441 ctagctttta aatcagcttc ttcaacaatt atcttagcct ttttattagc cgcgctacaa
     4501 gtgtcttctg ctgcactttg agcagttatt aaagtagctt ttagtgtttc ttcaatattt
     4561 tcatacttgt ttacttggtc ttgatataac cctaattttt ctttaagagc tgtattttcc
     4621 ctacatagag attcataatc ttccttgatg atatctaaaa attcatcaac ctcttcatct
     4681 ctataccctc ttaaaccttt tttaaactct ttattttcta tctcaattgg agttagcatt
     4741 acatttcctc ccagctataa tttaaattag tccttataca attaatcttg cttgaacttt
     4801 tatttttcct tttttggtaa tatctcctat ttgtgttacc ttagctcttc ctttgcctct
     4861 aatagaaatc aaatcatcat tttttactat tttagaagtt gaagttatct tttcataatt
     4921 gacatggact ttttctccat ttatgtattt tgcactatct tgtctagata tattgtaaat
     4981 accacttatc acacaatcta acctatctga agatactgta aatgatactt ctttatattt
     5041 tgaagaacta ttaacaatat tttctctgga aatttccttt acaattacat tatttcttga
     5101 aaccttttct aagtttacaa ttataaaatc gcaaatatca ctacttacaa ctacttgaca
     5161 aaagctatca tgtataatta tatctccaat tttttctctt tttataccta gacccagtat
     5221 tgaccctaag taatctttat gagatatatt tttaaacttg aaattccctt ctatttgtaa
     5281 aaatctaaga gtatcttcta tatcttcata ttccatgtaa aatggataaa taaatacagt
     5341 gcttctttca gcttgttcat aacctccatc cacactgtat tttatttcat ttgtagaatt
     5401 taatattgct acagcatttt tcacttcata tggattaaga aactccgtag acttaacatc
     5461 atagtttttt atacaactat tagccttgtc tatcacttta aacattttat ttttcaagtc
     5521 tatatccttt atgtgatttg ttagttttaa tttatccatc ctatcacctt agaatataac
     5581 caatactaat tgtcttagga acatcaataa tactattgca attaaaggag aaaaatctat
     5641 aggtccattt gtatatttat acattataga ccttattggc ccttctattg gctctgtaag
     5701 attttccaaa atattaaaaa ttttactttg tcttccattt ggaaaccaag tcatcaagct
     5761 ttttataact atcatccatg ctattatatc taaaagataa taaagtgcaa ttcttattgt
     5821 tcccatatat taaccgcctt tttactattt attttgccaa gggaagaatg ctttactttc
     5881 taattctttt ttcatatttg catctatatc cacattatta ggagctagta taaatattga
     5941 tttagaaacc ttttgtatac ttccatctaa tacataaact gccccattca taaagttaaa
     6001 tatagattta cgaacttcag aattagtcaa tccttccaaa ttcactataa ctgcccttct
     6061 ttgttttaga tggtctgcta tagttgcagc ttcatcataa accttaggtt caactattac
     6121 aactttcatt tggctagttg tatgtaaatt tacaatttta tttgcttttg cagtactaaa
     6181 gccactgttc atttcttctt cttgaactat atcgtccatg ccactttcat attcatcttc
     6241 aatataatct tcttcttcat caactatcca attcttaaat ttagatatta ttccatttga
     6301 cattattaac ttcctcctta aaatttattc aaatttgagt aattattttt tattgtagtt
     6361 tctttctcca aagattgatg ttccaactct aacaatggta gccccttcct ctatagcaat
     6421 ttcataatcg tgactcattc ccattgacaa aacatccatc cctacattag ggatgtttag
     6481 ttcttttatt tgttttgata aatctttaag acctttaaat acacctctta tctcatcttc
     6541 atcttctata aaaggtgcca ttgtcattag accctttatt tttatatttt tatatttttc
     6601 tgacacttct ttgacaaaat ctacaacttc ttctctatca agtccatgct tagactcttc
     6661 tttagatata ttcacttgta ctaagcaatc tatttctctg tttatttgtt ctgcacgttt
     6721 ttgaatttct tcgcataatg caattctatc taaagagtgt atcatacaaa ctttatcgat
     6781 tatgtactta actttattgg tttgcaaagt tcctatcagg tgccagttta atttgtcacc
     6841 tataatatca tactttctag ctaattcttg gggtttattt tcaccaacag aagtgattcc
     6901 ttccctcatt gcctcattaa ctaagtctac atcaacagtc tttgtgactg ccaaaagggt
     6961 aatctctttg ctgtctctat ctactttttt tgcagcttta tcaattttgt ttcttatctc
     7021 agacaaatta tctttaatag aacccattat tatcacctac tttatcctta tatttgtgtt
     7081 tatactgtta ggtcttaaaa ttattttgtc atatagatta acacttgtaa ctccatttat
     7141 gtcattttta taataatcca caaatacaga agattcattc tcataagtta ttccttctaa
     7201 tatgacaaat tttacacttt gtgattgttc atcaattaca tatacaccac gtttattgtc
     7261 ttttgttttt atagaagttt taggtatttt taaacattcc atctgattat atattatatc
     7321 aaattcttcg acacttgtat cctgatttgc aacaatttgg ttacttattt ttaattttac
     7381 cattatttta ttattatttt catatatttt gtaaactttt gcatctaatt tttgctcatg
     7441 atattcaatt attacatttt gatttttttc aaagttatta gaatcttctt tactgatgta
     7501 tgtagataga taattacttt ggtcattggc atctttttct atagttcctt ttacaggaat
     7561 ctttgcatca actttttcat tttttaacac caacgtatct acatttttgc taattatcat
     7621 tactaatata ttaaatacta aatatataag aataccaagt atcactaatc tggtatattt
     7681 tacctttttc aaagactctc cccctataaa attactttca ttataagtac ttcttctaaa
     7741 aaatgtttat tcctttttta taagctaata ttttacgttc taataattat tttttccata
     7801 ttaaatttat tttttatcaa tcttcctata caagactgca ttatttatat ataattgtat
     7861 tataaagtat aagtttaatt tacattttat acattaagct tactttatat gtttttatag
     7921 ttatttttat ttttagatgc aatataaaac caattaagat ttaaaataac ttttgttatg
     7981 ttgacattta tttaggtaat catttacgaa tcgggataac gttatcataa caaaagttta
     8041 tgtcctaaaa aaaatacaag gaggattttc ttatgtcaga aattttatta ggaacaggtt
     8101 tacttttatt ggttctatgt tgtttcacaa tttttagtta caaggcccca tatggtatga
     8161 aggctatggg agctctagca aacgctgcat gtgctacttt cttagtagag gcattcaacc
     8221 tttctttact tggtgatgtt ttaggtatgg aattttttag aagtgttgga gctgctaatg
     8281 gtagtcttgg tggagttgct gctgctatac ttgtgccttt agccttaggt gtatctccag
     8341 tttatgcagt tttaatagga ttatcttgtt tagatttttc tatattatct ggattcgttg
     8401 ctggatactt tgtttctttc ttaattaagt ttatagagaa aaaagtgcca cctggagtgg
     8461 atttagtggt tgtaattctt ctatgtgcac cgctatctcg tcttatagca tcaaatgtag
     8521 acccattagt taatagtaca ctactacaaa ttggacaagt tttgatagaa gctaccaaca
     8581 ttagccctat aataatggga attatactag gaggcataat aacagtagtt ggaacgtctc
     8641 cacttagttc aatggctctt acatctataa ttggtcttac tggtcttcct atggcaatag
     8701 gagcattagg tgcttttggt tcagctttta tgaactcaac actatttgca aaattgaaat
     8761 taggttctaa aaaagatatt atatctgttg ctattgaacc attaacccaa tcagacataa
     8821 tttcagctaa tccaatacct atatattcta ctaatttctt tggaggagcc ttttctggta
     8881 tcattgtagc cttgatgggt cttgtaaaca tgacaccagg tacagctaca cctgtggctg
     8941 gttttgctat aatgtttgct tataatccag ttcaacaagt tcttataaca gctggtttat
     9001 gtgctttatt caatgtaatt gcaggatttt taggttcaaa aatattcagt acctttaaag
     9061 ttcttactgc tggtgaaata cgtggtactg atgtagaaga agaaattgct taaaaaacaa
     9121 taagatttat aaatttatat ctcaagtagt agagttttat attgtaggca attttattaa
     9181 ttgcaaacgt tttatttttt atttatgttt atgattatta tgtgtgttga atatttagat
     9241 aaatttttat ttattatttt tatttagatt tttgtttagt tttttatttg gttgatatat
     9301 taggaataaa aattagtata gtttattatt agttagatag cataactttt tagatacata
     9361 aaaaatttta tattaattag atattattaa aataaaagcg atacatgaat ttttaaatgt
     9421 ccatgtatcg ctttattttt gccataatct taacaactga tttttaaagt ttattatctt
     9481 aacgaagttc attatcttat ataatattta aatg
//
LOCUS       DAELXX010000132         9414 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.134, whole genome shotgun sequence.
ACCESSION   DAELXX010000132 DAELXX010000000
VERSION     DAELXX010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9414
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.134"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            221..568
                     /locus_tag="KPH29_003204"
     CDS             221..568
                     /locus_tag="KPH29_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468776.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIAIFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            580..2559
                     /locus_tag="KPH29_003205"
     CDS             580..2559
                     /locus_tag="KPH29_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HBG1468777.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            2726..4099
                     /locus_tag="KPH29_003206"
     CDS             2726..4099
                     /locus_tag="KPH29_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBG1468778.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREASAQNAFVA
                     IVGENLDGHTFMKPAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHVYKLKTFGFNKNNDIYCESYTMEEDSLTFICVINGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            4140..5108
                     /locus_tag="KPH29_003207"
     CDS             4140..5108
                     /locus_tag="KPH29_003207"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBG1468779.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            5127..6482
                     /locus_tag="KPH29_003208"
     CDS             5127..6482
                     /locus_tag="KPH29_003208"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBG1468780.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASIIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSNNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEVVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            6534..7664
                     /gene="spoVE"
                     /locus_tag="KPH29_003209"
     CDS             6534..7664
                     /gene="spoVE"
                     /locus_tag="KPH29_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HBG1468781.1"
                     /translation="MPKENLKKQIDIRMKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            7682..8908
                     /gene="murG"
                     /locus_tag="KPH29_003210"
     CDS             7682..8908
                     /gene="murG"
                     /locus_tag="KPH29_003210"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBG1468782.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSTSKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
     gene            9178..>9414
                     /locus_tag="KPH29_003211"
     CDS             9178..>9414
                     /locus_tag="KPH29_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HBG1468783.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNINLNENIFAYNFKDMKNKLIK"
BASE COUNT         3740 a         1027 c         1807 g         2840 t
ORIGIN      
        1 atactagaag tgataaaaat aaatagaaat taatatctaa cctatagatt catataaaag
       61 tataagtgaa tttataggtt tttgttgtgt atagaaaaaa atacaattag attgtgtaat
      121 agtgtattaa tttagcgatg aaattttaga ggaagaaata tgattaataa caaatatagt
      181 atagaggaaa atttaaacta tatactatcg gggtgagaat ttgaaaaaaa attataaaat
      241 acataatcta aatgaatata aaaataaaaa aaagaataat tataaaaaga gaaataagaa
      301 aaaaatagga gctaaatgca ttttactgat tgcaattttt tcagtaataa tattgaatct
      361 atgtggttat tcaaaaatct cggaaatgaa gtacgatatc tattatttac aaaaagattt
      421 aagaaaaaaa gagatagtat tagaggaact aaaggcaaaa gaaactgaaa aaacatcaat
      481 aaaagaaatt gagctaaagg ctaaggaatt gctcaatatg gactatccaa aagatggaca
      541 aataaagtat atagatgtgg ataactaggg ggaatagatt tgagaaaagt aaagaggata
      601 agtaagaaaa ggctggtact cgttcttatt ctagcatgtg cattgttttt ttgccttgtt
      661 attaggactg ggtacctaca acttatgaag ggaaattggt tgagtacaaa agcactagaa
      721 caacaaacaa gagatatacc aatagaaccc aaaagaggaa ctatatatga tagaaatatg
      781 aaagaattag cagtaagtgt aactaagtat actgtttggt gtaaacctgt ggaagtagaa
      841 gataaaaaag aagcagctga aaaggtagct gagattttgg atgaagatta taaagatatt
      901 tacgctttaa taagcaaaaa aaatatggct cttgtaaaag taaaaagatg gatagatgat
      961 gataaggcaa gtcaaataag agatgctaaa ttaagtggaa tatgggtagc agaagataat
     1021 caaagatatt acccatatgg gaattttgcg ccatatgtgt taggacatac ttcttcagat
     1081 gcaacaggta tatctggtgt agaaatgcaa tatgataaaa agttaaaagg aaagcctgga
     1141 aaacttatag taagtactga tgcctcagga agagaaatac cacaaggaat ggaaaaatat
     1201 tatgagcctg tacaaggtaa tggtcttgtg ttgtctatag atgaagtaat tcaacactat
     1261 actgaaaaag cagtacagaa agcttatgag ttaaataatg caaaaaaggt tactgcaata
     1321 gctatgaacc ctaaaacagg tgatatactt gctttagcat caaaaccaga ttatgaccct
     1381 aatgactcaa gaacgccaat atatccatat tatcaagaag aacttgaaaa atataatgat
     1441 aaagataaaa taaaaggcta ttatcaaatg tggagaaatc cagcagtaag tgatacatat
     1501 gaaccaggtt ctacatttaa gcttattaca tcttccagtg ctcttgaaga gggagtaatt
     1561 aaagatggag aaaaattcac atgtacaggt agtgtaactg ttggaggaag aaagattaaa
     1621 tgttggagac attacagacc tcatggaaca caagaattta aacaagcagt acaaaactct
     1681 tgcaacccag tatttgttga attaggaagc agattaggtg ttggaaaaat gtatgattat
     1741 atagaatcat ttggccttat ggataagaca ggtatagatt taccaggaga agctaaggga
     1801 atactgtata acgaaaaaaa tgtaggacca gttgaattgg ctacaatatc atttggtcaa
     1861 tctatatcag taactccaat acaacttata acagctatat catcaatagc taatggtgga
     1921 gatttaatgc aaccaagagt ggtaaaatct tatactgaca ataaaggaaa tataactgaa
     1981 acagtaaaac caaaaaaagt aagaagtgtt atatctaaag aaacatctaa aaaaatgtta
     2041 gaaattgcag aatcagttgt aactgaaggt ggaggaaaga tagcatatat accagggtat
     2101 agacttggtg gtaaaacagg tacagctcaa aaagttatag atggtaagta tgctccagga
     2161 aagtatatat gttcatttgt tggaatagca ccttgtgatg acccgcaaat agtagttctt
     2221 gctattgttg acgaaccaac aggagtaagt gcatttggta gtactacagc aggaccaata
     2281 gtgaaagaaa taatgaatga ctctttaaaa tatttgggag ttaaaccagt atataaagaa
     2341 gaagaaaaag ctgagtatga aaagaagcaa gttaaagtgc cagatgtaag aaatctaaaa
     2401 ataggagatg ctgtaaaggc attggaggat gcgaagttaa aaccagactt agatgcagat
     2461 atagaacttc cagaagatac aaaggtaaag gatatattcc caaaacctgg agtaaaagtt
     2521 aatgaagact caagtataac cctatatttt gaaaactaaa aagtaacaga taattaacat
     2581 atacccataa acatcttcaa aaaaacagaa atatagtata taattataaa gttaagagtt
     2641 atcgtaggca atgtatatat aacggtatac attgcctttt gttgctaaga gaatatagct
     2701 aaggttttaa aggaggagtt taataatgga gtgcttaaca ataaaagagt tagtacttgc
     2761 aactaatggg aagttaatat atggagatta taatgactgt gtaagtgata tagttataga
     2821 tagcagggag gcaagtgccc aaaatgcatt tgtagccata gttggagaaa atttagatgg
     2881 gcatacattt atgaagcctg catatgatag tggatgtaaa acttttataa aaaatgaaag
     2941 taatggaata aaattagaaa gttcggatat aaatttaata gaggtaaaag atacatcttt
     3001 ggctctagga gatatttcaa aatactataa agaaaaattt gatattccat ttataggtgt
     3061 tacaggtagt gtgggaaaaa ctactactag agatatgatt tactctgcca tatctgcaaa
     3121 gttaaatata ctaaaaaatg aaaaaaactt aaataatcat tttggagttc cattgacttt
     3181 atttaatcta aataaagaac atgagtgtgc tgtaatagag atgggtatgt ctggattcaa
     3241 tgaaataaaa tatttagtgg atatagtaaa tcctaaaatt gcagtcatat cgaatatagg
     3301 attatcacat gtagaaaaac tgggttcaca agagggtatt ttgaaagcaa agatggagat
     3361 tacttcaaac tttgatgaaa caaatacttt aattgtaaat ggagatgata agtttttatc
     3421 tacattaaaa gaaaaagaac atgtatataa gttaaaaaca tttggcttta ataaaaacaa
     3481 tgatatttat tgtgaaagtt atactatgga agaggatagt ttaacattta tatgtgtaat
     3541 aaatgggaaa aaagaggaaa tatttatacc aacaatagga gaacataata tatataatgc
     3601 aatgtctgct atattagttg gtttgtgtct aaatatttct ttagattata taaaaaaagg
     3661 tttaaaaaat tttaaaggga caaaaatgag actagacata ataaaaaatg aaaaattaac
     3721 aataattaat gattcatata atgctagtcc agattctatg gatgctgctt taaagatact
     3781 tggaagatat aaaggtagaa gggtagcgat acttggagat atactagaaa tgggtgaaat
     3841 ttcagagtat ggtcatagat tagttggaaa atcatctatg aataatactg atattataat
     3901 aactataggt gaaaattcag tgtttattgg agaagaagca aaacaattag gattcaattc
     3961 agccaatata tatcactttg agaatagaga agatgtgttt aacaagttaa atgaattagt
     4021 aaaaacagga gacactattt tagtaaaagg ctcaagaggc atgagattag aaaaaatagt
     4081 cgaatatcta aataaataag cttaaactaa tgagttaatt gacaagggag aggagtttta
     4141 tgatgttagg aataacagag cttacgtaca ccgcattgat tgcgttttta atagtaatta
     4201 taataggacc aatatttata ccaatgctta gaaaatttaa atttggtcag acagttagag
     4261 atgatggacc ccaaacacat ctagctaaaa acggaacacc tactatgggt ggaattataa
     4321 tgattgttgc aattctaata actggactta caagagtaaa agttagtcat gatatggcag
     4381 taggtcttat atgtattgca ggttttggtt ttattggctt tttagatgat tttataaaaa
     4441 taaagctaaa aagatcactt ggactaaaag catatcaaaa aataatcctt caagttgcat
     4501 tatcttttta tgttgctttt tatcagtata catcatcatc aagtgcttca caacttatga
     4561 taccatttac agattttgtg ataaatgttg gaatattata tataccaata atgatgttta
     4621 taatcgtagc aatagtaaat gctgtaaact tgactgatgg attagatgga ttagcatcag
     4681 gagtaacatt aatagtttct gtattcttta tgctatttgc aagttctatt gctggaaata
     4741 cagaagtcgc agttttagca gcagcaactg ttggagcatg tttaggtttc ttaggattta
     4801 attcatatcc ggctagagtg tttatgggcg acacaggttc aatggcatta ggaggtgcag
     4861 ttgtagcatt ttcagttcta actaactcag tgcttataat acctataatt ggaggaattt
     4921 attttgcgga ggctctatca gttttgattc aagttggata ttttaaggca acaagaaaga
     4981 gattctttaa gatggctcca atacaccacc actttgaaca gtgtggttgg ccagaaacaa
     5041 gagttgtatt tattttttgg attataacag ttgttttagc ttggataagt ataatagcag
     5101 tattttaggg ctagggggaa tgaaaaatgg acttaaaagg aaaaaaagtt ttattagtag
     5161 gacttgcaaa aactggtata tctacaatta aacatttaga taaattgggt gcaagtataa
     5221 tcgtaaatga tataaaagat gaaaataaat tgagaaatat acttgatgaa ttgaaatcta
     5281 taaatgacat aaaatacata ttaggacatc acccagaaga tgtagatgat atagatatgg
     5341 ttgtagtgtc tccaggtgta ccactagacc taccattcat attgaaatta aaaaattcag
     5401 gcaagtatat aattggtgag gtagaattag cttttaaact atcgaataat ccaattttta
     5461 tagggattac aggtacaaat ggaaagacaa ctacaactag tttggtagga gaaatttttt
     5521 caagagcaaa acgagataca tatgtagttg gtaatatagg aaatcctgta atagatacta
     5581 tagaaacttc aagtgaagag tctgtgttag taactgaact aagcagtttt cagcttgaga
     5641 gtatagatga atttagacct aaagtaagcg caatattaaa tattacagag gaccacttaa
     5701 atagacacca tactatggaa aaatacatag aagcaaaggc aaatatattt atgaaccaaa
     5761 ctgttgagga tttctgtgtg ttaaactatg atgatgaggt agtaaaatct ttagctgata
     5821 aatgcaatgc caaagtaatt tacttttcaa gaactaaaaa agtaaatgga ggagtatatt
     5881 tagaaaataa tgacataata atagatatag atgataaaat caaatttcta aataaagatg
     5941 atgtaagttt acctggaggt cataacttag aaaattgtat ggcagctata gcaatagcat
     6001 atgtctgcaa aattgattta gaagttataa aggatgtact tatgacattt aaaggagtgg
     6061 aacacagaca agagtttgta agaaatttag acaatgtaat atatgtaaat gattcaaagg
     6121 gaactaatcc agattccact ataaaagcta tacagtctta tgatagacct ataatactta
     6181 tagcaggggg gatggataag ggaagtaatt ttgatgaact tcttgaaact gcaaagtcat
     6241 atgttaaatc attggtctta ttaggagaaa ctgcatcaaa tatagaaaat tgtgctaaaa
     6301 ataaaggatt taatgacata catatagtaa aagatatgga agaagcagtt aagacttctt
     6361 atgaaatttc aaaaagtgga gatatagtgc ttctttcacc agcttgtgca agttgggata
     6421 tgtatgaaag ttttgaagta agaggaaaag acttcaaaga taatgtaaac aatttaaaat
     6481 aatctcttac ctctaaactt acttaaatgg aagaatggag gtagattagt tggatgccta
     6541 aagaaaattt gaaaaaacaa attgacataa ggatgaagac tgaatttgat ggagtagtgt
     6601 tttacactac tatgctttta gtatttgtag gaatagttat ggtgtttagt gcaagtttta
     6661 tacaatcttc atttaaacac aatgatgcgt actattttct aaaaaagaat gtaatatatg
     6721 ctgtacttgg atttatagtt atgattatta catcaagaat agattatagt ttttggaaga
     6781 aaaatgctac agcaattgga gctatagcag tagtattatt attgttagtt ttgacccctt
     6841 tagggataga agcaaacgga gcaaaaagat ggttgggaat aggagcttta acatttcaac
     6901 ctgcagaaat cgcaaagttt gctacaataa tattaactgc aaaattaatt gaaaaaaatt
     6961 atgataaaat aaaatctttg acaaaaggtg ttgtaccact tctcgtagtt ccagggatat
     7021 tttttgcgtt gataatactt cagccaaatt tgtcaacagc aggtactgta attctagtta
     7081 cttttgtaat gatatttgtt gctggaatgg atatgaaaat tgtatttgct atgataggga
     7141 gtggggctgc tttatttgct gcattagtaa ttgctgaacc ctatagactt agtcgtgtta
     7201 catcattttt agatcctttt caagaccctc ttggtaaggg gtatcaggta atacaaggtc
     7261 tatatgcact aggttcaggt ggattgtttg gtctaggctt aggaaagagt aaacaaaagt
     7321 atttttatat accagagcca caaaatgact ttatatttgc tataatagga gaagagttag
     7381 gattgattgg ttgtataata gttattatgc tatttgtagt tttagtctac agatgtgtaa
     7441 gaatagcttt gaagacttct aatgtatttg cttgtatggt tgttataggt ataggtgcac
     7501 aaattggaat acaagcagct ttaaatatag cagttgcgac atcatcaatg ccagctacag
     7561 gtgtagcact accatttata agttatggag gaacttcact caccatcttt atgggagctg
     7621 taggaatagt gttaaacatc tcaaagcatg tcaaaattaa ttaacaggag atgatgattt
     7681 gatgaaagtt ttattatcag gcggtggaac tggagggcac gtgtatccag ctatagctat
     7741 tgcaaacaaa ataagagatg agcatccaga cgctgagata atttttgttg ggacggaaaa
     7801 gggaatagag tcagaaatag ttcctaagta tggatttgaa ttaaagactg taacagttca
     7861 aggttttaag cgaaaaatag atttcgataa tgtaaaaaga gtttttaagc tttttaaagg
     7921 tttggagcaa tcaagaaaga ttgttaagaa atttaaacca gatatagtta taggaactgg
     7981 tggatatgtt agtggacctg ttttatttaa tgcatctatg gggaaaatac cagccataat
     8041 acatgagcaa aattcttttc ctggtgtaac taataagatt ttatctaaaa cagttacaaa
     8101 ggtacttaca agttttgaag attcgcataa aagatttcca gaggcagcag aagataaatt
     8161 agtttttaca ggaaatccag ttagaaaaga aattttatta tcaagaaaaa atattgcaag
     8221 aaagaatcta tcaataagtg acgaaaaaag aatggtactt tgctatggtg gtagtggagg
     8281 ttcaagaaaa ataaatgatg caatgagact tgtaataaag aatatggtaa atgaagatat
     8341 agcttttata tttgctacag gcaaaagcta ttatgatgaa tttatgggaa gtattagcga
     8401 tataaatctt aaaccatacc aaaaagttgt gccttactta gaagatatgg caaatgcact
     8461 tgcagcaagt gatttggtta taggtagcgc aggagcaatt tcattagctg agataacggc
     8521 tcttggaaag ccatctataa taatacctaa agcttatact gctgaaaatc atcaagagta
     8581 taatgccaag agtatagaaa aacaaggggc aggaattgct attttagaaa aaaatctaac
     8641 tccagagagc ctaaatacag cagtatttaa acttttagga gatagagaac ttttagttga
     8701 tatggcaaat gcaagtaaaa ctataggtaa gcctgaagcc atagacctta tatatgatga
     8761 gataatgaaa gtatacaatt caacacaaaa gtcaacttct aaaaaaacaa aaaaggaaaa
     8821 agttattaag gaagtaaaag aaataaaaaa agaaacgact ccaagtatag aaggtcaagc
     8881 taaggtaata ggaataaaga aaagataagt aaatatttac ccctaattta taatatatta
     8941 gattttctaa attattataa attaggggtt tatttgtatg tatacagatt taaatatata
     9001 aatagtatgt agtatactat gtttattttg atgaaatatt gatttattta taaaatttaa
     9061 taccttattt ggatgttatg taataaaatg caaaaatttg actttaaata ttataatttt
     9121 tttgtaggaa aaacaagctc taaaggtgta tttattaacc aaaggggttg attatatttg
     9181 aaaaaaagaa gaaagttaaa tacgaataat gtcatgatag ttttagtttt tatacttatg
     9241 ttatcttttt gtatatgtat aattgtaaaa agtgatttat ttgatgtaaa gaaaatagat
     9301 gtaattggaa ataagagggt gaccaaatca aatataatga aagaactaaa tataaatcta
     9361 aatgaaaata tttttgcata caattttaaa gatatgaaaa ataaacttat aaaa
//
LOCUS       DAELXX010000133         9395 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.135, whole genome shotgun sequence.
ACCESSION   DAELXX010000133 DAELXX010000000
VERSION     DAELXX010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9395
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.135"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            245..1873
                     /gene="aspD"
                     /locus_tag="KPH29_003212"
     CDS             245..1873
                     /gene="aspD"
                     /locus_tag="KPH29_003212"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HBG1468784.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDEI
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEDLFKFISKTASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
     gene            1994..2989
                     /locus_tag="KPH29_003213"
     CDS             1994..2989
                     /locus_tag="KPH29_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBG1468785.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGIGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(3147..3815)
                     /locus_tag="KPH29_003214"
     CDS             complement(3147..3815)
                     /locus_tag="KPH29_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG1468786.1"
                     /translation="METVSNNQSSVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFSEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            complement(3914..4226)
                     /locus_tag="KPH29_003215"
                     /pseudo
     CDS             complement(3914..4226)
                     /locus_tag="KPH29_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431045.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4653..7073)
                     /locus_tag="KPH29_003216"
     CDS             complement(4653..7073)
                     /locus_tag="KPH29_003216"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBG1468787.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDTLDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
     gene            complement(7427..7777)
                     /gene="rsfS"
                     /locus_tag="KPH29_003217"
     CDS             complement(7427..7777)
                     /gene="rsfS"
                     /locus_tag="KPH29_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBG1468788.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            complement(7894..8466)
                     /gene="yqeK"
                     /locus_tag="KPH29_003218"
     CDS             complement(7894..8466)
                     /gene="yqeK"
                     /locus_tag="KPH29_003218"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBG1468789.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSRNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            complement(8467..9156)
                     /locus_tag="KPH29_003219"
     CDS             complement(8467..9156)
                     /locus_tag="KPH29_003219"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBG1468790.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
BASE COUNT         3111 a         1560 c         1230 g         3494 t
ORIGIN      
        1 aataaaatac aataaaaaaa ctaaggtgat aattaaattg aatatttatt ttatataaca
       61 atcatttttt attaaaaggt attttttatt ttaacatata tttaataagg ctaaatagag
      121 ttattatttt aatttcatac tttacttttt ctataaacct attataatta aagtataatc
      181 atttttgcaa cttgtttatt ttattataaa aatgtgaaac ttattacata aggaggaatt
      241 taacatgaaa aatcatactt ataaagaaat caaaaacata tatggaaaaa taagcccttt
      301 tgaatttaag gacaaattaa tagatattgc aaaatacact gcaaaagaaa ataacagaga
      361 attacttgat gcaggtcgtg gaaatccaaa ctggacttgt tcaactgcta gagaagcctt
      421 ttttactttt ggtcatttcg ctattactga aactcgttct aattgggatt taggtcattt
      481 ggctggaatg cctcaaaaaa agggtattaa agaaagattt tttaaattta taaatgaaaa
      541 tatagatatg cctggagcat atttagctag agatattata aactttggta taaatgaact
      601 tggatttgat ggagatgaat ttgttcatga actagctgat ggaattattg gtgataacta
      661 tccacttcca gatagaatgt tacctcatat ggaaaaaata gtacacgatt acctagttca
      721 agaaatgaaa tatgatatat caggtaaata tggtgatgta gaaatttttg ctgttgaagg
      781 tgctacagct gctatgtgct atatatttga ttcattaatg gcaaatgaac ttctaaaaaa
      841 aggagataca atagccttaa tgactccaat atttacacct tatcttgaaa tacctaacct
      901 tcctcgttac gactttaaag tggtaaatat aaatgctaat gaagttgatg aaaaaggtgc
      961 tcatacatgg caatatacaa aaaaagaatt agagaaactt cgtgacaaat ctattaaagc
     1021 tttattcgtt gttaatccta ataatcctgc ttctatagct atggatgaaa tatcttgtaa
     1081 taatctaata gatgtaattg aaaattataa taaagattta atgataatat cagatgatgt
     1141 atatggaact tttgtagaag atttttcatc cttaatgtca aaactacctt acaacactgt
     1201 tggagtatat tcttattcaa aatattttgg agttacagga tggagacttg gtacatttgc
     1261 ccttcataag aaaaatgttt ttgataagaa aataaatgat ttaactggag aattaaaaaa
     1321 atcagtagat aagcgttaca gtgatatgag tcttaaccct tctagtcttt catttatgga
     1381 aagagttgtt gctgatagca gacttgttgc tctcaaccat actgctggtc tttctactcc
     1441 acagcaagta caaatggcgt ttttctctgc ttttgcattg attgacaaag tagatgccta
     1501 caaaaaactc aatatgagta tctgccatag acgtcaaaaa ctattatttg aagctttaga
     1561 gcttccaatt aatgaaaaca aaaacaatgc tgcatattac actcaatttg atattgaaga
     1621 atgggctaaa ttaaactacg gagaagactt atttaaattt ataagtaaaa ctgcatctcc
     1681 agtagatgtt ctttataaat tagctaatga ctattcagta gttttactta gtggaaatgg
     1741 attctatggc cctgaatggt caattagaat atctctagca aatctatatg atgaagctta
     1801 tactaagata ggaaaagcca taagagagat attaaatggt tatatcgttg aatggcaaaa
     1861 aattaagaaa taattatatt acactgaaat atctaaatta tatgaataaa agagtttatg
     1921 tagaccttaa ctactttttt tgatataatc ctatcggaat tataaatttt atttatgaaa
     1981 ggcagagaat cgtatgagta ttaagaaaaa agttgccata gtttttactg gtggaactat
     2041 atctatgaca gtagatgata aagtcggagc agctatacca actttatcag gtgaacaaat
     2101 actatcaatg gttaccaaca ttgataaagt tgcagatgtt gaagttttta attttgatga
     2161 aattccagga cctcacataa caccttatag aatgatggaa ttgaaaaatt atgtaaatga
     2221 tttattagca agaaaagata ttacaggtgt tgtaataaca catggtacag atagtttaga
     2281 ggaaacagct tattttctag accttacaat tgacaacatc aaacctgtaa tagtcacagg
     2341 tgctatgaga agtagttctg aacttggata tgatggttca agtaatttat ctgcatcagt
     2401 ttgtactgca gtatccaaag atgctatggg aaaaggagtc cttgtagttt taaacaatga
     2461 agtattatta gcttctgaag ctactaaaac aaatactctt tctctaaata catttaaatc
     2521 tctaactagt ggtccattag gaataattga ctgcaacgaa cttgtcctta caagagatat
     2581 tgtaaataga acaattatag atactgataa ggtagaatct aaagttgcct tatttaaagc
     2641 atatgtagga gaaaatgctg actttataag atttgctgtt gatagtggtt ataaaggtat
     2701 agttattgag gcaatgggta taggtaatat tcctcctcaa atgctttctg gagttgaata
     2761 tgctagagaa aaaggacttc ctgtagttat agtttctaga tgtcattcag gtagagtatt
     2821 cgatagttat ggttactttg gttctggtag agatttaaaa aatcttggtt gtatatttgg
     2881 tggggacttg ccaggtcaaa aagctagaat aaaacttatc ttagcacttg gaaaaactga
     2941 taaccttgat gaaatcaaag atttctttga aaaaggaatc tattgctaat tctaataact
     3001 tctatttaat tatttagttc agtttacaag aaaatacaca caaaccataa aatgagatgt
     3061 tgacaacata acagacagta tgctcctgta aaaggtaaca tactatctgt tatattgtct
     3121 ggattcccaa attagctatt tggaacttaa ctaaaaaatc ctaatataag ggcaactatt
     3181 ataatggcaa tcggaataca tactgtgtaa tcctgttcca tagagtcatc atctgaatgt
     3241 agttttacaa ttcgcaattt agcaatcagt ttgaagcaga aatagcctat tattgttcca
     3301 attacattcg tgagtaagtc gtctatatcg gttgctctgt gtaacgtaaa aagctgaata
     3361 gtttcgataa agagagataa tccaaaacca atcaagaatg tatttctcaa ccgttcaaag
     3421 gttttgctga taagcggaca taaaaaacct aatggaacaa aaagtaaaat attcaaaata
     3481 aaactcaaac taaatccatc actaaaagga ataagattta tgtttggatt aaagaacgtt
     3541 tcccctaact gtgacagtct tatatattca ctgaatgtag ggataccaac aatatgcgat
     3601 agtgttaaac ataagtagta atacattgcc agtgataaaa tagatgtttt tacctttgga
     3661 atatcactat ttttcttgaa tattacaact aagaaaatac caatcaagat aattcccaac
     3721 aagaaaatag gattaattaa caataaatct ttgatagctt caaacagact gagttgaatt
     3781 tctacacttg attgattatt tgatacagtc tccatttgta cacaccccct cataacattt
     3841 caattatttc taaaaaacaa aatagaagat aaatcttaaa atcatcctat caaaattttt
     3901 aaatttgcct ttatcagttt ataccaccag ttaattcctg tcttacttct gtatggtatt
     3961 cttgggattc tttcatcaaa ttagacaaaa aaatggttac catatcaata aactggttac
     4021 gctccatatc tcctgaagca aacatcccct gagaataaat ccgtatcagg aatctccaca
     4081 tctactgcac ctatcacaag tcatttgata acatcctcaa acaatcccaa cttggatata
     4141 tcagcaacac ccattagaat actactgctg accatccttg ttttcctggt gataatttta
     4201 atcaactggg aagttgaaac gcctatcttt tttgcaagtt cctttttaga aacatggtag
     4261 ttattatatg aatctgaatc tcgttgtctg ggtgtttaag ataaagcata gatacgcacc
     4321 ttttttgcct gttttcatta tataaaaaaa attttaaaaa tacaataatt atattttttt
     4381 cgtttaagct tagagcagtt ccttgatgaa caacacttta gaaactgaat aatttacata
     4441 ccacttaata cattaacacg atttaacatt aattataata atatttaaaa caacaattag
     4501 agtgtataag aatgaacttt ttagttcttt aatcattttt ttgtataaaa tcaaatctat
     4561 ttttgtaatt tcaacaatga aaaaaggctt atctctattt tgagacagcc tcatttattt
     4621 tataaattta tatttttgtt catatttttg agttatttaa caactatatt tactaatttt
     4681 ttaggtacag ctacaacttt aactatagtt ttaccttcaa ctaatccttt tattttttca
     4741 tcttctaaag ctaccttttc catctcatct ttagatatat ttgagttaac agttaatttt
     4801 cctctaactt ttccattaac ttgtacaaca acttctattt catcttttac taaagctttt
     4861 tcgtcatatt taggccagtc tatatcaaat acacttcctt ctttacctat catagtccat
     4921 agttcttcac ctaaatgagg ggcaaatggt gcaattatag ttactatagt ttgtacacct
     4981 tcttttataa ctgcttcgtt tatattatct aactctttat atttatacat atcatttatt
     5041 aattccataa gagctgatat agcagtatta aatccgaatt tttcacttaa atcagcagta
     5101 acttttttaa gtgtagagtg tatagtatat ctcatatcct tatcttggga atttaattct
     5161 ccaaactcta catccttttt aaccacatct gctaactcgt caaccaatct ataaacccta
     5221 tttaagaatc taaagcatcc atcaacacct tgctctgacc agtctaaatc tctttctggt
     5281 ggtgctgcaa ataatacgaa aagtcttgca gtatctgctc catactcatc tattatatcc
     5341 ataggagata ctgtattccc tttagactta ctcattttag aaccatccat aagtaccatt
     5401 ccttgagtaa gtaagttttt aaatggctca ttgaagtcta ccattcccat actcttaaat
     5461 gcctttacaa accatcttgc ataaagcaaa tgcattatag catgttctac tccacctata
     5521 tattggtcta ctggatgcca tctattaact aattccttac taaatggttc attttcatta
     5581 ttgctatcca catatcttaa gaaataccaa gaagaatcaa cgaatgtatc catagtatca
     5641 acttcccttc tagctggttt tccacagtga ggacaagttg ttgacataaa ttgctttgaa
     5701 gtcgtaagtg gagattctcc ctttccagta aattcaacat ctgttggtaa aagaactggt
     5761 aaatcttcct ttttaactgg aactattcca cattcatcac aataaaccac aggtatagga
     5821 caaccccagt atctttgtct tgaaactaac cagtctctaa gtctataatt aattgttctc
     5881 tttcctctac cttctttttc taacttatcg attattcctt caaatccttc tgaagcatct
     5941 aatccattaa attcttctga atttatcatt ttgttatctt ctgttattac atcaattatg
     6001 tctaaattat atttttcagc aaaatctcta tctctttcat catgtgctgg aactgccatt
     6061 atagcacccg ttccatactc aactaataca tagttagcta tccatagagg aactttcttg
     6121 ccatttaaag gatttataac atatctacct ataaacattc cttctttttc tacatcagct
     6181 gctgttcttt ctatttctgt catagtatgc attttttgaa caaaagcttc aacatctgct
     6241 tcatattcag tccctgcaac caattcttta actaattcat gctctggagc aagtaccata
     6301 taagtaactc cataagttgt gtctggtcta gttgtaaata ctgtcataga tttatcagtt
     6361 ccatctatat caaaaactaa atctgctcct gtacttttac ctatccaatt tttttgcatt
     6421 gttttaactt tttctggcca tccatctaat gtatctaagt cattaagtaa ttcttctgca
     6481 aattcagtag tcttaaaata ccattgttct aaatctttct ttaatactgt tgctttacat
     6541 ctctcacatg ctccttgaac aacttgctca tttgcaagaa cagtctcaca agaaggacac
     6601 cagttaacat atgatttttt cttatatgca agcccatgct ctaaaaactt caaaaatatt
     6661 tcttgtgtaa atctatagta ttctggagtt gaagttgcaa cttctttatc ccaatcataa
     6721 ctaataccta ataaatttaa ttgctctttc atttcttcta tattttccat tgtccattta
     6781 tgcggatgta ttccatgttt tatagctgca ttttcagctg gtaatccaaa agaatcccat
     6841 cccattggat gaagtacatt atatccttcc atttttttga atcttgcgac aacatcacct
     6901 atagaataat ttctaacatg tcccatgtgt atttttccag atggataagg gaacatttct
     6961 aatgtgtaat aattaggttt ttcagtttgt gcagtatcca ttttatactg attattatct
     7021 ttccaaattt tttgccattt cgattctact tctttaaaat tatatacgct catattttgc
     7081 ctcctatttt taattgtgta taactacctt atttagtatt atttacttat aatttccaaa
     7141 taattctttg aaaataaaaa atcctcgtca ctaaacttac gtttagggac gaggataaat
     7201 cttcgcggta ccaccctaat tagcctacta catatataga ctctcttaga tagcttttaa
     7261 tccagccatg gagaatagat tttaatctag taagttcaaa gttatataat attagttctc
     7321 accttccact aactctctga aaaaatataa tcttttactg ctctagtcta ttcaatttaa
     7381 tataattttt gaaattttct aattataata acatatatta tcatgtttat gctaatgtgt
     7441 caatatctat ataaggtgca tctttccata atttttctaa attataaaac cctctatttt
     7501 cctcattaaa tacatgaacc attatgtctc cataatcaag gagtacccaa gtttgagttc
     7561 cttgtccttc tttacctctt ggctctaatc ctagttttgc taattcatct tccacattgt
     7621 cagcaatagc ttttacctgt ctctgagaag atgctgtagt aataataaaa taatcacaca
     7681 aactagaaac ttttcctata tttattataa ctgtatcttg acccagttta tcttctatgg
     7741 catcatatgc tatttttgtc atttgttcca ctgtcatata aaaattcctc actttcctaa
     7801 ttaaaataca cttagttatt gtaacatatt aatacgattt tgtaacatag tcaaatttaa
     7861 atttattaat atgtttgcct ttatatagct tttttataat aacttttctt taattagata
     7921 gtttcttgcc ataactgttc ttggatgtat ctcttctttt ttattgatga caaacattaa
     7981 agtattatta aaagaagtta aaagtgcttc atcaagcttc cctccatatg ctaattctct
     8041 taatgtatct acaactggaa attttcttcc ttcttcaatt aaatcagcta tgtaaattat
     8101 tttttctaaa agagacatgt cctctctccc tgttgtatga tatttgattg catttataat
     8161 atcttcatct tctacttcaa attcatactc gcatatgtac gccccaatta cactatgaga
     8221 caaggaacga tttccatcct ctattgggtc taaatatatt tcatacttat ttacataatc
     8281 ctctatttcc ttgtcactta aatactttgc acaatcatga atcattcctg ctaagtaagc
     8341 tttttctttg tcacatccat aaatctcaca taatttttca gcacattttg caacatttct
     8401 tgaatggcta agcctcccca ctggtagcat ttgatttagt ctttggttaa tattttctaa
     8461 gttcatttaa tcatctccat attgatatag tttattttca tataaataat tttctacatt
     8521 ttcgggtact aagtaacgta ttgacttgcc ttcatttagc tgttccctta tatatgtgga
     8581 cgatatatcc agtgatggaa cataaactgt tataatgtca gcattatact tcttagttag
     8641 tttttctata gtctcttgtg accttaacaa actaattcct ggtctagttg cggctataaa
     8701 agtagctaac tcaaagtttt tctttacatc tttccactct tcaacactac aaattgcatc
     8761 tgcacctgtt ataaaataaa tgtctgcatt tttgtatttt ttctttaagt attttaatgt
     8821 atctattgta taagtccttt tctttctttc gatttctact tttgaaacta aaaaatcttc
     8881 gttgctttca gttgctagaa gtgtcatatt atatctatca tacttatctg tagtgataca
     8941 taatttatgt ggtggatttc ctgatggtat aaagattatt ttgtcaatgt catacttgtc
     9001 tctaataaat tctgctgttg ctaaatgagc atagtgtata gggtcaaaag tacctcccaa
     9061 tatacctatt ttgattttgc ctttatggcg attaaatttc tctagttttt cagtattttt
     9121 caactctgcc atcttaacta gattttcact tctcatattt tgtctccttt taatgttata
     9181 atactattgt accattattt tttatttctt aaagcttata atttgttttt ttgggtatat
     9241 aattatattt atatgctaaa aaacacttta aaaaataatt atttaacatg attctgcttt
     9301 actttttaat aaaaataact ttttactatt ttgtcttaat tttgataaaa tataacatac
     9361 aaatataagt tttttgttaa attttattag ttgct
//
LOCUS       DAELXX010000134         9382 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.136, whole genome shotgun sequence.
ACCESSION   DAELXX010000134 DAELXX010000000
VERSION     DAELXX010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9382
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.136"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(384..1787)
                     /locus_tag="KPH29_003220"
     CDS             complement(384..1787)
                     /locus_tag="KPH29_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG1468791.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
     gene            2664..3173
                     /locus_tag="KPH29_003221"
     CDS             2664..3173
                     /locus_tag="KPH29_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBG1468792.1"
                     /translation="MSKELAINEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLD
                     LVQISPNANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTK
                     ANNAIKFLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNN
                     MVVIIDPKK"
     gene            3201..3395
                     /gene="rpmI"
                     /locus_tag="KPH29_003222"
     CDS             3201..3395
                     /gene="rpmI"
                     /locus_tag="KPH29_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBG1468793.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            3451..3807
                     /gene="rplT"
                     /locus_tag="KPH29_003223"
     CDS             3451..3807
                     /gene="rplT"
                     /locus_tag="KPH29_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBG1468794.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGIEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            4117..4662
                     /locus_tag="KPH29_003224"
     CDS             4117..4662
                     /locus_tag="KPH29_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468795.1"
                     /translation="MNEKLMDCFVNPIKCKLLLEVFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKEGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGVIITPPKEDEGK"
     gene            4649..4822
                     /locus_tag="KPH29_003225"
     CDS             4649..4822
                     /locus_tag="KPH29_003225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468796.1"
                     /translation="MKGNKSDLHEVEPIYTENRFMIYICIQSKVSLHGLYFIVPFHPM
                     TWSGTMKFFNFSY"
     gene            4822..4998
                     /locus_tag="KPH29_003226"
     CDS             4822..4998
                     /locus_tag="KPH29_003226"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468797.1"
                     /translation="MPKEMNFRPHMQLGYIIQLDQIYRIKISIFIADKLTVILLKNHL
                     LVIVSILKEIIVIH"
     gene            5098..7023
                     /locus_tag="KPH29_003227"
     CDS             5098..7023
                     /locus_tag="KPH29_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG1468798.1"
                     /translation="MKIKFVIMPLVIFLIVLFVGCQSTKDTEVKAKDTEIKILGTGDL
                     HSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFISNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWIINDKFSKTIEVE"
     gene            7424..9328
                     /locus_tag="KPH29_003228"
     CDS             7424..9328
                     /locus_tag="KPH29_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG1468799.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFKQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVKVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKV
                     FGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEGKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSSSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
BASE COUNT         3751 a         1041 c         1640 g         2950 t
ORIGIN      
        1 aagtgataga tacctaatta agttgtagat atctgataag aaatttttaa gttttcaatc
       61 gttctaaatt gtgagtataa aaatctacta aatttagtta tgtaaaatat aaattataag
      121 tgtagaaact aggctactag cttttatacc acttcaaaca ttactggaag tagttaagtt
      181 tacagcagtt tagttttatg tagatataat attctgtaaa tagacaaaat taaaatatgt
      241 aattaaaata tgtaagtgtc tcaaaatgaa atttttaatt tatgaggcac ttttttgtat
      301 aaaatcaaat atatttttat aactttaaca ataaaaagag gcttatctct attttgagac
      361 agcctcaaat ttttaaaact atattagttt acagcttctt ttcctatcaa atcttttaac
      421 tcttcatgag tcatagtttc tttttcaaat aagaatttag aaacagaatg taaatcttca
      481 agattatcct ttaacatctc taaagtctct ttataacaaa tttcaactat acgatttgct
      541 tccttttgaa ccttatcaca taagaaagtc ttatcatttc catcaatagt cataaatcct
      601 aagttactca taccatattc acaaaccatt tggttagcta tttcagtgac tttctttaag
      661 tcgtcctttg caccagtaga tttatgatta aatattaatt cttctgaagc ctttccagca
      721 agtaatattt ttatttttcc aatcaactca tccttagtat aaatatatct atcttcatca
      781 ggtaattgaa gaacataacc taatgcttgt cctctaggaa ctatagatat tttttgtata
      841 ggacaaattc ctactatatc acttacaaga gcatgtccag cttcatgata tgctactatt
      901 tttttctctt tttcaaccaa tgcagagttt ttagattcta gccctgcaat aactctttca
      961 agtgccttgt caaaatgttc tgatgtaata cactcactgt catctctaac tgcaaatata
     1021 gctgcttcat ttgctatatt tgaaagatga gccccattga aaccatgagt ttttttagct
     1081 aaaagttcta gatttacaga tttgtctata ggtttgtcat ctgtatgtac ctttagaatt
     1141 tcaaatctag tgtgatagtt tggtgcacct atatgaatgt gtctgtcaaa tcttcctggt
     1201 ctaagtaaag cttcatctaa taagtcaagt ctattggtag cacctattat taatacattg
     1261 gaatctttat taaatccatc catttcaact aaaagttggt ttagtgtttg gtctttttcg
     1321 ttgttacttt caagatgtct ctttgctcct acagcatcta tttcgtctat aaatattata
     1381 cttggagctt cttttctagc tttttcaaat agagttctaa ctctttttgc tcccacacct
     1441 acatattttt caacgaactc tgaaccagta acattgaaaa atgatgagtt tgtttcacca
     1501 gctacagcag atgcaagtag ggttttacct gttcctggag gaccataaaa taatactcct
     1561 ttaggtattt ttgcacccat tttttgatat ttttgtggtg acttcataaa atcgactatt
     1621 tcaaatagtt cttcttttac ttcgtctaat ccagcaacgt ctctaaatgt agtctttggt
     1681 tttgtagaaa gtgaatcatc tgtttgatta tctctttcca tttttgtctg tgcaaagaca
     1741 ggagctggat tgttgatttt cttaaaaaag ttgtttagca ttttcatatt cacacctcag
     1801 gtaattaatt attgttaatc aattttatat tttccatatt tactattttt tattatttat
     1861 ttttcatgga aaaaatatac atgttatcta aataaaaata aatttgtaag atgtatgaaa
     1921 aaatgaattt atgtatatag agatttctac ttatgtattg aataaagtat tacaaattat
     1981 aaattatgta ttgattatat attgagtaaa gtattataaa gcatgtattg attaagtatt
     2041 tattaagtat tgaataaaat attacaaatt tagttgaaat gaaaatgata aaaaatgttg
     2101 tgctatattt tacaaatcat ataaatagag aaattgttgt agcacaaagt aataggtagt
     2161 atattagaat ataatatagg atttgttgtg tagtatagta gcatattaga atataatata
     2221 ggatttgtta tgtagtatag tagcatatta gaatataata taggatttgt tatgtatata
     2281 gtagcatatt agaatataat gtaggatttg ttatgtagaa tatatagagt catgtagaat
     2341 ataaatatat aaaaggattt tgggtataaa tttaaaaatt aatagcaaaa tgagtagtat
     2401 agacatataa ttagtagtag gaagtaatta aatttttcct aaacaaaaaa aatagttgca
     2461 aataatttga ttaagtgata tagtaaataa ggtaagaata aaacaaagaa gaagtccgct
     2521 tctcacctta cgccggaggt tagtaaggta aatataagtt aaagcttaat tttaatttaa
     2581 gtttgtttaa atttggtaac ttatattgcg gatgattacg tcatccgctt tttattttat
     2641 aaaaattttg gaggtgtcct acaattagca aagaattagc aatcaatgaa caaataaaag
     2701 ataaggaaat aagagtcctt tcaccaacag gtgaacaact tggagtaatg cctactaaag
     2761 aagctcaagc aatggctaat agcaaaaact tagatttggt tcaaatctcg ccgaatgcga
     2821 atccaccagt atgtaagata atggattatg gaaagtttag atatgagcaa gcgagaaaag
     2881 agaaagaagc taagaaaaaa caaaaaacta tagttgtaaa agaagtaaga cttagaccag
     2941 gtattgaaca aaatgattta aatacgaaag ctaataatgc tataaagttc cttaaaaaag
     3001 gggataaagt caaagttgaa cttagattta gaggaagaga attaggacat aaagacatag
     3061 gtaaagaagt tatgcttaag tttttagata taataaaaga gtttggagaa ccaacaaaag
     3121 cccctgcatt tgagggtaat aatatggttg taattataga tccaaaaaaa tagatgatta
     3181 actgagagga ggaatttgtc atgcctaaaa tgaaaactca tagaggtgca gcaaagagat
     3241 taaagaaaac aggaactgga aagttaaaga gagctaaagc ttttaaaaaa catatattaa
     3301 ctaaaaaatc tgcaaaaact aaaatgaact taagaaaatc aactttagta agtgatggag
     3361 atgccaagag aatagcacaa ttattaccat actaagatac agattgtaaa taataactat
     3421 agaagaggga tataaaagga ggtcataacg atggcaagag ttaaaaaagc tatgaacgct
     3481 cgtaaaaagc ataagaaaat attaaaactt gcaaaaggat tcagaggatc tagaagcaaa
     3541 ttatatagac cagcaaatac attcgtaatg aaagcattaa aaaatgctta tataggaaga
     3601 aagttaaaga agagagactt cagaaaactt tggatacaaa gaataaatgc agctgctaga
     3661 atgaatggaa tatcttattc aagattaatg aatggattaa aattatctgg tattgaagtt
     3721 aacagaaaaa tgttatctga aatggctata caagacccag aaggatttgc aaaattagca
     3781 gaagttgcta aagcaaaatt agcttagtat tgaatcatga gatgtaaagt ctttgagtta
     3841 tataaataat tcaaggactt ttttattttt tgaaaaatac ttattagtgt catgtacaac
     3901 caattttata atgataaata aaataagcaa tacaatagaa ttttatgaga tttattataa
     3961 gaattgttac aattatttat catagctata aaaagtcata gtaaaaatga tattgatgta
     4021 tgtttatata tgtataaagc tttcaatcaa tcttgaatta atattatcaa catgataata
     4081 ttatcttatt aataatataa ataaggggga gataatatga atgagaaact aatggattgt
     4141 tttgtaaacc caattaaatg caagttattg cttgaagttt tttcaaataa tcgaactaca
     4201 gctaaacaat taggagaaaa atttcaagat atatcaaagg caacacttta tcgttacctt
     4261 aataaaatgt tgaaagatga tattataaaa gtggttgaag aaaataaaat aaggggaact
     4321 gttgaaaaag tatatgctat gaagttagat atagctgatg atgtgaacaa aattatagat
     4381 gagaactctg gagaagaata tgctcaactg tttactcaat atatgatggg atttttaaga
     4441 gaattttatg aatacgctga aagaaaggaa aaggatatcc taaaagaggg ttcaggattt
     4501 tctgtctgtc cagtatatgc tagtattgat gaaatagttg aagtgagtaa aaaaattgct
     4561 agtatactag aacctctaag aaataactcg cacgaagaag gcagaagaat gcattctata
     4621 ggagtgataa tcactccacc taaagaggat gaagggaaat aaatctgatt tacatgaggt
     4681 agagccaatt tacacggaaa atcgttttat gatttacata tgtatccaga gcaaagtatc
     4741 tctgcatgga ttgtacttca ttgtaccatt tcatccaatg acttggagtg gaacaatgaa
     4801 gttttttaat ttttcatatt aatgcctaaa gaaatgaact ttagaccaca tatgcaatta
     4861 ggctacatta tacaattaga ccagatatac agaataaaaa ttagtatttt tatagcagat
     4921 aaattgactg ttattcttct taaaaatcat ctattagtta ttgttagcat attgaaagaa
     4981 attatagtaa tccattgaat aaatgaatat tgcccataat catataaact gctatagttt
     5041 aaaataaagc aggacgttaa attttatagt aatcttaaaa tttaagaggg ggattttatg
     5101 aaaataaaat ttgttattat gccattggtg atatttttaa tagtattatt tgtggggtgt
     5161 caaagtacaa aagatacaga agttaaagca aaagatacag aaattaaaat attaggtaca
     5221 ggtgatttgc attcaatttt gacagactct atggtttcat atgtaaatga ggaaagggaa
     5281 aagaataaaa atctgcttat ggtagatgcg ggagatttct atggtacaca aagtagagaa
     5341 atgtgggaat ggtcttctgg aaaaaagtta attaatatta aaagaaatgg tagagcagag
     5401 tatgatgaca taattaaaag tagtaaagat gaagtaccta tagttaaaga tatggcaaag
     5461 ttgaaatatg atgctgtagt tttgggagat aatgaattta tttcaaatga taagcaaagt
     5521 cttgataaac tagtttctga ttttaaaaat aataatatgc cattggtatc agcaaatata
     5581 tatgaacaat ctggagaaaa ttatgtacaa ccatatgtaa tgaaaaatat taagacagat
     5641 gagggcaatg taaaagttgg aatactaggt cttactataa aagaagtagg ggaaaccctt
     5701 ggtcttgagg attttgaaaa ggataaaaaa gctagagagc ttggagaaca agctgattac
     5761 aaaggaaaat tatatgcaaa tgatttagta gaagatgcaa agaaatggat aaaagttatg
     5821 gaaaaagaaa gtccagacat aatagttgca gttgtacact caggggaaga acctaaaaca
     5881 gatagaaatc caggaaatag aataaaagag ttagcaacta cagtggatgg aatagatgca
     5941 attgtagctg gtcatactca tgaaaaaata gatgaacaca aatataaaaa taaatctgga
     6001 gatgaagtaa ttgttacaca accaggttgg catggagata gagtatcaga gattaatttc
     6061 aaattaaata aagagaatgg aaagtgggac ataaaggaaa agtctagtaa attaaaaata
     6121 attgataaag aagtaagtat agttgctact gcagatttac attcacattt ctctgataaa
     6181 ttaacagttg accttgttaa tgaacgtagt aacccaattg aacctgttgt agtagatgca
     6241 ggcgacttct tagaccctca aacagatgaa atgactcaat ggtataaaga gtggaaaagt
     6301 ataagagata ataatagtga taaaacgatt agtagatgcc caatggtcat ggatatgaat
     6361 cagggaatgt atgatgcagt agtacttgga aatcatgaat ttgtatctga aaaagatgaa
     6421 ttttggtcag atgaacgcct tttagatgct gttatagagg attttgaaca ggtggcaatt
     6481 tctgtgttat cagcaaatat atataggcaa tctggtaaaa attatgttaa accatatatt
     6541 ataaaagatg tggagacgag tgaaggtaat gtaaaagtag gaatattggg acttactata
     6601 aaagaagtag gtaaaggtct taaaaatgta aacttacaag agcaatttca atataaggat
     6661 aaattgtatg caaatgattt agtggaagat gcaaagaaat gggtaaaagt tatgaaaaag
     6721 aaaaaaccag atataatagt tgcagttgta cattcaggag aagaaccaaa aaatccaaaa
     6781 cacccaggaa atagaataaa agagctagca actacagtag atggtattga tgcaattgtt
     6841 gctagtcata ctcatgaaaa aatagatgag catgaatata aaaataagtc tggagaaaaa
     6901 gttatagtga cacaaccagg tgagcatggt aaatattatt ctaaaattac ctttaaagta
     6961 aataaagaaa agggttcgtg gattattaat gataaattca gtaaaacaat tgaggttgaa
     7021 taactttata aaatttatag ataaagtaaa ttatctgata ttgacatgat atataagata
     7081 aagtttctaa tattagtata atgcataatg tgaaatattt taataataag agaataaaca
     7141 agacaaataa aaatatttta acatgtaaga tatagtatga actattatac taatgcttac
     7201 tttataattt gaacatgtta gaataagtct tatttgaaaa ttttagtgta ttagagtaag
     7261 tattatttta ccagtaaaaa atattgagaa ctatatgtag atttgcttaa aaaggaattt
     7321 acatatagtt cttttcgtat aataaatagt acaatcttat atggtaaaga taaagtgtga
     7381 tattaaattt tattatcatc caaaatctaa ggaggagtct cttatgaaaa taaaatttat
     7441 tatagtagca tcaataatat ttttaatagt attacttgtg gggtgtcaaa gtactaaaga
     7501 cacagaaatc aatgtactcg ccacaagtga tttacattca attattccag ataatttaat
     7561 ttcctatgta gaagaggaga gaaaatatga tgagaatcta cttatggtag atgcaggaga
     7621 tttttttgac atgcaaagtg gtgagatgaa taagtggttt actggtcaaa aattagtaag
     7681 atttaaagat gggataccag agtacaaaaa aatagctgaa ccacgtgaag gagaagttcc
     7741 attggctaaa aaaatggcaa agctgaagta tgatgcagtt acatttggga atcatgaatt
     7801 tgtatctaat gacaagcaga gccttgataa attggtttct gattttaaaa ataacaatat
     7861 accattagta tcagcaaacg tatatgaaca atctggagaa aactacgtaa agccatatgt
     7921 aatgaaaaat ataaatactg acaagggaac tgtaaaggtt ggtatacttg gtctgactat
     7981 aaaagaagta ggggaacgtt caaggtttaa aagttttaaa caagataaaa aggctagaga
     8041 acttggggaa caacctcaat acaaagggaa attatatgca aatgatttag tagaagatgc
     8101 aaataagtgg gtaaaggtta tggaaaaaaa agaaaagcca gatataatag ttgccattgt
     8161 acattcaggt gaagagccaa aaaatcctaa aaatccagga aatagaataa aagaactagc
     8221 aacaactgta gagggaatag atgctattgt agcaggtcat acacatgaaa aaatagaaca
     8281 gcatacatat aaaaataatt ctggtgaaga agtaatagta acacagcctg gagctcatgg
     8341 taatagtata tcaaagatta attttaagtt ggataagaag aatgataagt ggtttataaa
     8401 agacaaatat agtgaattaa aggtttttgg aaaggaagta aatataatta caacttctgg
     8461 tctgaatcca aaattttcag atgaaatggc agttaatttg tttaatgaaa gagatagaga
     8521 tgttcaacct atctttatag atactggaga ttttttaaat actaatacaa aagaaatgac
     8581 taagtgggct aaagagtggc aatacaatga aaataaaggc atctataaaa aaataaatga
     8641 atggccaatt gtatatcttg gttatgatgc tgtagtactt ggtagccatg aatttacaga
     8701 ggataaagag acttttgagg gaaaaaaatc tacattggat tatattatag aaaactttga
     8761 agaaatgaag ataccagtat tatcagctaa tatatatgat gaatctggag aaaattatgt
     8821 aaaaccttat ataattcata atgtagaaac tagggaagga aatataaagg ttggagtatt
     8881 aggtcttact actactgata aggatgaaga agacttaagt agctcaagct taagcaaaga
     8941 ctcaaagact tcgaaaattc ctgaagaaac aggaaagtta tatactaata atctagtaaa
     9001 agatgcaagc gaatgggtaa aagttatgca aaaggaaagt cctgatgtga ttgttgcagt
     9061 tgtacattca gataataata gtactctaaa aaatccaagt agtgaagtca aaaaacttgc
     9121 aatgtcagta gatggaatag atgctattgt tgtaggtcat acgaaaaata aggtagaaga
     9181 acatatttac aaaaataagt ctggagatga ggttatagta acacaatctg gagaagattg
     9241 ttattcaaaa ataagtcttg aactaagaaa agaaaatggc aagtggaata tcataaacaa
     9301 atatagtaaa gcaataaaaa tgaagtgaat ttattgatat ataaaccatt attaacactg
     9361 atttttataa tataataaat ac
//
LOCUS       DAELXX010000135         9213 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.137, whole genome shotgun sequence.
ACCESSION   DAELXX010000135 DAELXX010000000
VERSION     DAELXX010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9213
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.137"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            184..1389
                     /locus_tag="KPH29_003229"
     CDS             184..1389
                     /locus_tag="KPH29_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBG1468800.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVGPSKIRTFGIPVEKSFLSHRDKD
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNVKSIRKSNSAQNIA
                     NLILNIISG"
     gene            complement(1460..1681)
                     /locus_tag="KPH29_003230"
     CDS             complement(1460..1681)
                     /locus_tag="KPH29_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468801.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
     gene            complement(1822..2421)
                     /gene="recR"
                     /locus_tag="KPH29_003231"
     CDS             complement(1822..2421)
                     /gene="recR"
                     /locus_tag="KPH29_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBG1468802.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            complement(2434..2784)
                     /locus_tag="KPH29_003232"
     CDS             complement(2434..2784)
                     /locus_tag="KPH29_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBG1468803.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            complement(2843..4480)
                     /gene="dnaX"
                     /locus_tag="KPH29_003233"
     CDS             complement(2843..4480)
                     /gene="dnaX"
                     /locus_tag="KPH29_003233"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBG1468804.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKYSIDENESK"
     gene            complement(4557..4821)
                     /gene="ffs"
                     /locus_tag="KPH29_003234"
     ncRNA           complement(4557..4821)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="KPH29_003234"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(4859..4950)
                     /locus_tag="KPH29_003235"
     tRNA            complement(4859..4950)
                     /locus_tag="KPH29_003235"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4914..4916),aa:Ser,seq:gga)
     gene            complement(4951..5406)
                     /locus_tag="KPH29_003236"
     CDS             complement(4951..5406)
                     /locus_tag="KPH29_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HBG1468805.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
     gene            complement(5726..6997)
                     /gene="serS"
                     /locus_tag="KPH29_003237"
     CDS             complement(5726..6997)
                     /gene="serS"
                     /locus_tag="KPH29_003237"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBG1468806.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKTRLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEYKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
     misc_binding    complement(7122..7328)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7455..8336)
                     /locus_tag="KPH29_003238"
     CDS             complement(7455..8336)
                     /locus_tag="KPH29_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBG1468807.1"
                     /translation="MNFLSLAKAASTNSNITKRTLENMLNNFSDNIPNIIYAIIVFVI
                     GIYISKIVRKMVSRFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGVIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFINEKR
                     ILDDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENNIKVPFVP
                     SSLLFNNMSNSNTMDKR"
     gene            complement(8348..8857)
                     /locus_tag="KPH29_003239"
     CDS             complement(8348..8857)
                     /locus_tag="KPH29_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBG1468808.1"
                     /translation="MNSDERRSKLIDILKESKCPVKGGMLAEILNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHRNSKEIYDELKIIVDLGGIVKDVIVNH
                     PTYGQITAELNIHSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIETLKEETIEVITK
                     ELEDRGILS"
     gene            complement(8984..9100)
                     /gene="rrf"
                     /locus_tag="KPH29_003240"
     rRNA            complement(8984..9100)
                     /gene="rrf"
                     /locus_tag="KPH29_003240"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         2944 a         1628 c         1006 g         3635 t
ORIGIN      
        1 atatttttaa tataacatat ttgataaaat taatttacat ttctttcact tgtaattttt
       61 gcaatcttgt attagattaa tattaaaaat aatttatcaa taaccttatt cacaaagaca
      121 gtttttatat atcaaaatat aatacaatat aaatactatg ctacatttta ggaggatttt
      181 attatgagca aaaaagtatt aataatgtct gcttccactg gtggtgggca taatagggct
      241 gctctagcta ttaaagaaga acttacttcc aaaacacttg atggagagcc aattgaatgt
      301 gaaataatag atagtttgaa attagttaat aatacaatgg ataaaataat ttcaagagga
      361 tacgaaaaat ctgctatata tactcccaaa gcctatggaa gtgtatatag attatctgaa
      421 acaaatcttt tgtcaaaaaa tgaatttaaa gataatctac taataacatt catggctaaa
      481 aaatttaaaa agcttattag aagcgaaaaa ccagacttga ttataggaac acatcctttt
      541 ccaatgattg ctctaagcac attgaaaaaa aactttaatt tacataataa tgaatcaaat
      601 gcttacacag agcattttta taaacattat acaaatacca ttaatgttcc tccgcttatt
      661 tctgtattaa ccgattatac tactcattct acatggatac aaaatgaaat tgattactat
      721 attgttggtc atgaatatgt aaaagaatta ttggtgtttg atggagttgg accgagtaaa
      781 attagaacat ttggaatccc tgttgaaaaa tcttttctct ctcacagaga taaagatatt
      841 gttctttctg agttgaattt atctcctgat aaattaactg ttttacttat ggggggtagc
      901 tttggggctg gaaatataaa agagacactt gatgaattac tggatactga tagagatttt
      961 caaatacttg taattactgg taagaatgaa agcctaaaag aaaaaatcga aaaaaaactt
     1021 atgtctcgtt atcatgataa aaatgtatgt gttcttggat acactgataa gatgaatgat
     1081 attttagctt ctatagatgt attagtaagc aaacctggtg gacttactac aactgaggct
     1141 cttttaaaag atgtacctat gatagttcct tattatatac caggtcagga agaagagaat
     1201 cttgatttct tatctaattg tggtgcagca cttagaacta caaaaaaata taacttgtca
     1261 atattactaa aagttctaat agatgaccct agcagattag aaatgctaaa aagaaatgtt
     1321 aaatcaataa gaaagtcaaa ttcagcccaa aatatagcta atttaatttt aaatataatt
     1381 tctggttgat aaaataaata aaatccctat agtatatata ttatctatta gggatttttt
     1441 attttctaat tatttaatat taaattggtt cataaaagtc ttcttcataa tcatactgat
     1501 aatttttata attatttcta aattcagcat ttttatttcg tacatttttt ttagtttttt
     1561 tcttattaga aaaacttata tcctcaaaat catcatattt aggtttatct tttgatttca
     1621 tttttttaac tcttttttct ttcattgcat tatgttcttc taagttcata attggtttca
     1681 tgttgcaccc ctatcaaaat ttttaaatga tttatacaac aaattataca atacagaaga
     1741 aatttattca ataaatctaa atcaaaatta atttgaattt aactcaaaaa actacaaaaa
     1801 tcgaggtatt aacctcgatt tttatatttc tcttctgcct tctagtgctt ttgatattgt
     1861 gacctcatct gcatactcta aatcgcctcc aacaggaaga ccatgagcta tccgagtgac
     1921 tttgattccc attggtttta ctaacctagc aatatacatg gctgtcgctt caccttcaat
     1981 agtaggattt gttgccatta ttatttcttt gacttcctga ttgccaagtc tttggataag
     2041 ttcctttatt tttaacatat ctggtccaat tccatccatt ggagaaatta ctccatttaa
     2101 tacatgatac tgtcctttaa actctctcgt tctctccata gctgctacat ctcttggatc
     2161 ttccacaaca catataacac ttgaatctct acttttattt gaacacatgc tacatgggtc
     2221 tgtatctgtt atattacaac aaattgagca atatcttatt tctctttttg catctataat
     2281 cgctttagat aaagcttcca catcattagt attcatatta ataatatgaa aagccaatct
     2341 ttgagcagtc tttcttccta ctccaggaag ctttgaaaat tcctctataa gtctcgttat
     2401 gggacctgta taaacttgca taaataccac ctactagaat aacccaggta tattcatacc
     2461 tccagttacc ttactcattt gacttgcttg tatatcatca atatttctca atgcttgatt
     2521 tactgcactt aaaactaaat cttgtagcat ttctatatca tctggatcaa ctacttctgg
     2581 cttgatagtt atatctataa cttctttttt accattaact tttacagtaa ctgctcctcc
     2641 accaactgaa gcttctactt ctttactttc taactcttgt tgagcttttt gcatattttc
     2701 ttgcattttt tgagcctgct ttaacaagtt gttcatattt ccaccaccag gcatcattcc
     2761 gcctccaaaa ccttttttag ccataaacac tattcctcct aaacttattt tcattttatt
     2821 attatatcat aatagtattt tattatttac tctcattttc atcaatacta tattttattt
     2881 ctaatatctc ttcactcact atgttcttta atatctcctc accaatgtcc tttttctctt
     2941 taacttcaag atttatatct ttaacttctg atgtcagtac tatttttaca ttaaaacttc
     3001 tatttaaaac atctcttatt gttttttcta aataatttat tgtatcttga gagctcagtc
     3061 tagtttttgc aaaatcaaat ttatcatcaa atataacata caacatatta gaatatacat
     3121 taaaactttt tacttcactt aagagtgcgt atattggcat ttttttatct tttttaattt
     3181 gtttaagtat attcttccaa gatgactcta taagtataac gtcctcattt tttacatctt
     3241 cataaactac attttcttgt tttacatcta tttctctatc gacctcatac tctttttctt
     3301 tattattccc aattttttca gacttaaagt tccccagttc aatcattttt tctaagttct
     3361 caactctttt tataagagct tctttacttt catcaaacat tggttgagca attttcatta
     3421 tggttatttc cactaaaacc cttggatttg aagatgactt tatatcatcc tgagttattg
     3481 ataatatatt taaaatcctt attaaatcat ttatatctat atttttagac tgaattttaa
     3541 gttgttctat agtttcttct ggtaatgaaa ttatttcatc caattctgat gaaaccttac
     3601 ataccattaa atttctaaaa tgatcaatta agtcatttat aagatttctt atatctttgc
     3661 cccataagac aaattcattt aatatctcaa gagacttttt agtgtcctgc tctacaatac
     3721 attgagacaa ttcaaataat tgttctatat ttacgcttcc cattaattca acaacatctt
     3781 tataatctat cttactttct ccaaaagaca tacattggtc taatatacta agtgcatctc
     3841 taagagctcc ttgagaattt ctagctatta aattaagagc tttatcatct atatcaattc
     3901 cttcatcttc acaaatcttt ttcattctaa gggtcatatc tttaactgta actcttttaa
     3961 aatcgtatct ttggcatctt gataaaatgg ttgcaggtat tttgtgaggc tctgtagttg
     4021 ctaatataaa aattacatat gatggaggtt cctctaaggt ctttaataat gcattaaaag
     4081 ctccttgaga caacatatga acttcatcta ttatatatac tttatattta gcattggctg
     4141 gagaatattt cacactttct ctaagctctc ttatatcatc tacactatta tttgaagctg
     4201 catctatttc tacaacatcc attgtattat cttttaatac agattcacat atctcacact
     4261 cattacacgg ttcttcattt attttattca aacagtttac tgctcttgaa aaaatctttg
     4321 cagtagatgt tttaccagta cctcttgttc cacaaaacaa ataagcatgt cctatattat
     4381 cactatatat ttgatttttt aaagtcttaa taatatgatc ttgaccaata acatcttcaa
     4441 atttttgtgg tctatatgct ctatataatg ctttatgcat ctttttacct ctctttagca
     4501 aaattaataa aaaaatccct cattatctaa gggattagtc ttttatatat taaatgctgt
     4561 acaccatcta tcgacttaat tccataggcg ttaccaaaac agttagctca acttaggcac
     4621 ccccacggca cacaaaagtg atcacttacc gctgcttcct tccggatctg acggggttca
     4681 tgagctttca ttgcgcagga cctaagcatc aacaccactt atttagggcg gccctacaga
     4741 aaataagcct ttagatggaa ttcaatcctg ctatagcgga ttgcaggtta cagggcaccg
     4801 ctacctcccc atctagtaca gcaaaattac aaccatattt aattactttc attgtttctg
     4861 gcggagagag tgggatttga acccacgata cagttttacc catatactcg ctttccaggc
     4921 gagcgccttc gaccactcga ccatctctcc ttaatctctt tttctcaatg ccttaaaaaa
     4981 ttcttgcaat atgatcgagc attcttcttg caaaacatca aaagctactt ttacattatt
     5041 attattaaaa taatctaatt taaactcatg ctgtttttct atatatgaat tttttacatg
     5101 tcttgtccct attataagct tttttattct agaattaact attgcgccac tacacattat
     5161 gcaaggttcc attgtaacat ataaatcaca atcagtcaac ctccaaccac ctaatttttc
     5221 tgatgcttgt tttatcgcta aaatttctgc atgtgccgta gagtctttta acgtttctgt
     5281 aaggttatga gctctagcta ttatttgatt atctttaact ataatagcac ctattggtgt
     5341 ttctttttta ttatatgctt tatatgcttc ttttaatgct tctttcatat aaaatgaact
     5401 ttccataatt cctcccaaaa cttataacaa acttatctta tcataattca ttttataatt
     5461 caacttaatt ttattttgtt tatttaatta ttactatata tagcgatatt ttttttaatt
     5521 agttctaaaa taatttttag atattcatac aagagtgttt tataaaaata tttagtgttt
     5581 tatgatgaaa attctgctgt gtaaattaac ataaaaaaac tatgaaatta atatttataa
     5641 taatttcata gtttttttat ttattgttta ctcataatat attctcttca tcaaagcata
     5701 ctctacatat acaattaata taaatttatt ttataacatc aacacccata taaggtctta
     5761 attcttctgg aactactaca gaaccatctg cctgctgata attttctaat atagcagcca
     5821 aacatcttcc tatcgctaat ccagaaccat ttaatgtatg aacatattct gcttttgatt
     5881 ttttatctct tttaaatctt attcctgctc ttcttgcttg gaaatcttca aaatttgaac
     5941 aagaagatat ttctacatat ctattgtaac ttggcatcca aacttctaaa tcatatttaa
     6001 atgcagctgt aaatcccaaa tctcctgtac atatttttac aactctatat ggaagaccta
     6061 acatttgtaa catagtttca gcatcatgag ttaatgattc taattcatta taagaatctt
     6121 ctggtttaac aaatttaact aattcaactt tattaaattg atgttgtcta actaaacctc
     6181 ttgtatctct tcctgctgaa cctgcttcag atctaaaaca aggagtatat gcacagtatt
     6241 taattggaag ctctgcaaca tctaatattt cattagcatg tatattagta acaggaactt
     6301 cagctgttgg tattaagaaa tactctaatc cttctatttt aaacatatct tcttcaaatt
     6361 taggaagttg accagtccct ataaaactat ttctattggc cataaatgga ggtaagactt
     6421 ctgtatagcc atgcttagct gtatgagtat ttaaaaagaa attcattaaa gatctttcta
     6481 atctagctcc taatccccta tataaagtaa accttgaccc agttattttc cctgctcttt
     6541 caaaatctaa aatacctaga cctgtcccta tatcccaatg cgctttatac tcaaaatcaa
     6601 atttagtagg ttctccccaa gttcttattt gaacattgtc ttcatctgtt tcaccttgtg
     6661 gtacctctgg atgaggaaca tttggtattc tcattaatgt atattccatt tttgcttcta
     6721 cttcttttac tttctcatca atacctttta tcttatctga taactctttc aatcttgtct
     6781 tttcttctgt tacatctttt ccttctttta ttaattgagg tattttttta gaatttacat
     6841 ttaactcatt tttcataact tcaacttctt gaagtatttc tcttctttta ttatctagtt
     6901 ccactacagc atcaaggtca aattctcttt cacctcttct ttccatagct tttttgatat
     6961 catctagatt ttctcttatt cttttaatat ctaacatatt aattcctcct aataattttg
     7021 ataatataaa ttataattcc aattaattta caagtattct agctgtaaac ttttaattta
     7081 aacaaatttg aaaaataaaa aaagacctcc gtttacccta taaaagggac gaaaatcttt
     7141 tatccgcgtt gccaccctaa ttgattatac taaaacgtat aatcctctca gacctataac
     7201 gtaggtaaac cgtctagctt taactagaag ctctagagta gattcaaaaa ccattgtaat
     7261 gagttctcac catccactca ttctctgaat acaatctgtt ttttactatt ttccttcacc
     7321 gctttttaac taattgtttt atttgataat cattatatta tatatattaa catctttcaa
     7381 tactattatt caaatttttt tagaattttc tttaattttt tatattaaat tcactttttt
     7441 acttaattga aattctatct tttatccatt gtatttgaat tgctcatatt attaaataat
     7501 aacgaggaag gaacaaatgg aacttttata ttattttcgt caaatttatt tttgactttt
     7561 tccattacat caaagtaaac atcccagtaa tcatcagttt taacccatgg tctagcaact
     7621 atttgcatag tgttgtttcc tatagagttt aaacctataa ttggttttgg gtcatccaat
     7681 atcctttttt catttataaa aatttcattc aatatggact taactacatc aatatcacta
     7741 tcatatgaaa tattatatga aaaatctatc cttcttttat tttgatgtgt gtaattaatt
     7801 atattgttac ttgtcaattg aaagtttggt ataattatgg ttttattatc tggggtttta
     7861 agaacagtac taaatatttg tatatcagaa actgtgcctt ctacacctga gccttgaata
     7921 taatcaccta tattaaatgg cttaaaaaat aaaataatca tacctgaacc taaatttgat
     7981 aatatttctt taaatgctaa acctatacta aatccagcag ctcctaatac agctacaact
     8041 gatgtagttt gtatccctat aacacccaag cttatcaatg atataataga taacatagat
     8101 atgtatattc catatacaat gaaattattt acacctttac tcattccata tttgattaaa
     8161 aaccttgaaa ccatctttct tactatctta gatatataga tacctattac aaatacgatt
     8221 attgcatata taatatttgg tatattatca ctaaagttat taagcatatt ttctaaagtt
     8281 cttttagtta tattactatt tgttgaagca gccttagcta atgataaaaa attcatttaa
     8341 ctcctcctta tgataatatt cctctgtcct ctaattcttt tgttataact tctatagtct
     8401 cttcttttaa tgtctcaatt gtatgaatat gactacattc agttagaact gataactgtt
     8461 taaattcatt tgtctcaacc ttcttcataa aattatctat atccatttta gagtgaatat
     8521 taagctctgc agttatctga ccatatgttg gatgatttac gatgacatcc ttaacaattc
     8581 cacctaaatc tacaataatc tttaattcat cataaatttc tttagaattc ctgtgattct
     8641 tacattttat ttgctttgta aaatatggtt gattatatat tatatatcct tgaggtgtag
     8701 ctattatttc aaatcctcta gctcttataa gggctatatc ttgtactatt acttgcctac
     8761 ttacatttaa tatctcagct agcattccac cttttacagg gcacttagat tcttttaaaa
     8821 tatctattaa tttgcttctt ctttcatcag aattcatatt acaccttctt tctaaatcaa
     8881 aaactttacc tatactatat aattctatat tttatcttat tataaatcaa tattgatttt
     8941 atattctatt taatattaaa ctttattgca aataaaaaag attacgtggc tacgtcctac
     9001 tctcccaggc agtttcccac caagtaccat cagcgctaga gagcttaact tctgtgttcg
     9061 gaatgggaac aggtgtatcc tctctgctat tgtaaccaca taatctttat gcttaatatt
     9121 tctattaagt ttttagaatc tagtaatact agtttcttta gagttaacac tctaaaaact
     9181 acatatatat tttattgaat ggacatcaaa taa
//
LOCUS       DAELXX010000136         9073 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.138, whole genome shotgun sequence.
ACCESSION   DAELXX010000136 DAELXX010000000
VERSION     DAELXX010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9073
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.138"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            258..914
                     /locus_tag="KPH29_003241"
     CDS             258..914
                     /locus_tag="KPH29_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468809.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGARILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(1384..2655)
                     /gene="sleC"
                     /locus_tag="KPH29_003242"
     CDS             complement(1384..2655)
                     /gene="sleC"
                     /locus_tag="KPH29_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic protein SleC"
                     /protein_id="HBG1468810.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIVAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGADTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            2900..3616
                     /locus_tag="KPH29_003243"
     CDS             2900..3616
                     /locus_tag="KPH29_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HBG1468811.1"
                     /translation="MKLDLFLLAIVPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            3695..5005
                     /locus_tag="KPH29_003244"
     CDS             3695..5005
                     /locus_tag="KPH29_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBG1468812.1"
                     /translation="MTELPKEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            5105..5641
                     /gene="lepB"
                     /locus_tag="KPH29_003245"
     CDS             5105..5641
                     /gene="lepB"
                     /locus_tag="KPH29_003245"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBG1468813.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            5807..6343
                     /gene="lepB"
                     /locus_tag="KPH29_003246"
     CDS             5807..6343
                     /gene="lepB"
                     /locus_tag="KPH29_003246"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBG1468814.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            6353..7108
                     /locus_tag="KPH29_003247"
     CDS             6353..7108
                     /locus_tag="KPH29_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HBG1468815.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELKGFDIRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EVLTLDAEYLVLEINYEDIEDTISKLNNIVREG"
     gene            7182..8849
                     /locus_tag="KPH29_003248"
     CDS             7182..8849
                     /locus_tag="KPH29_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HBG1468816.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVILIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
BASE COUNT         3394 a          943 c         1694 g         3042 t
ORIGIN      
        1 gatgatggta gataagaata tcagaaaaaa atagataatt aaagggttag ggtgaaagag
       61 taatcgtaaa ataaatatat agataatggt ggatagagta gtaagaaaat aaatagataa
      121 agggatagaa taaatttaaa tatggaaaat gaatatctaa taataagtga ttaataaata
      181 attgtagata gattgtaaat taaataaatc tttaatagaa aggagaacta tttatatatt
      241 aaatagtatt tataaatatg aaaaaaggcg attttatatg gagtggagtt cttatttgta
      301 ttattgctgt cttagcaata ccaagtagta gagaagtatt tatggaagca acaggtgcac
      361 atccatatat tgggggtttt ttaaagtttg ctgtattagc tacaatggga gatttacttg
      421 gtgctagaat tttgtataag gattggaaaa taccagtagg agtattttat agagcaatag
      481 tttggggaat aataggtgta gttaccacat atggaatgtc aatctactca tcaggtgtag
      541 gtcaagcaca agtggatggt atgcttccat ttgagggaat agcttttgca aatgctctat
      601 ttaagagtgc aactatgaat atactaatgt caccaactgt atttttattc cataaggtta
      661 tggactgttt tatagataaa aaatatgagg ttggaaaggg gattaaggta aaacttaaag
      721 atgtggtaaa tattgtggac tggaatgctt accttggttt tagtgtttta aaaactatac
      781 catttttctg gataccatgt catacaattg tattcttatt ccctgctgag tatagagtaa
      841 ttgcttctgc atttgcatct atagctttag gtctaatatt ggctttagct aataagtcta
      901 aagttgcagt ttaggttcac tgatgaagat aatagtatta tttaaattat gaataacttt
      961 ttaaattaat attatttata tttataataa ataataattt agataagaag gaatattcta
     1021 agtgatagat agtgttttaa ataataagga gtaatattct aagtgagaag tagcatttta
     1081 aataataagt gagattttaa ataaaaagta agcttttcgt tagaatagca ttttattaaa
     1141 cttaaaaata aatataatat tttaaagaaa tctaagttgc taagcactgt ttttaagaag
     1201 cttaaaatcc cccatttaaa gttttaagat tctaaaaaaa caagttataa ctaaaaaatg
     1261 gctgttaaga ctacaatact tataagtaaa aatacttatg gtatatatta gttcataaca
     1321 gccattttta ttttaaatta ataattttat tcaataattt gagtttatgt tctaaatcaa
     1381 gctttaaatt aaaggattta aagaagctat tctagttgta gctacataaa ctctggatat
     1441 ttcataccat gttttaaaat ctactactcc gctttgaggt aaaccaaaga ttccttggaa
     1501 tatcttaaca gcgttttctg tatctgctcc atagatacca tcttctataa cttttggtac
     1561 agctggataa gaattagata tagcatttaa ctgattttgt atggttctaa catactgccc
     1621 agaagaacca acttgtagtg tagtaccagg gaatgacact ggtacaccac ttactatagg
     1681 tgctctttca aatactatct catctccata gaaatatctt aggatactct catagtcata
     1741 tccttggtca cctaagtctt tactgcccca ttgagtcatt tggtcaggac attgagattt
     1801 ttgtccatca caatattgtg ctaatagtgg ctgtcttgat gtaggaggtc tttttataaa
     1861 agtattaaaa atagcatcta caactacgtt aattggttca aataagtttc tattatttat
     1921 aaacttatgg tcgtatgctg ttgttgaagt tattgtaaag ttatatcctt tatttctata
     1981 ccattcagta aagactctat ttaaagtaaa tgatattata gctattacat ttgcatagat
     2041 tgtctgttca ggccaagttg aatatatttc agatgacgct atgtttttaa tgtactcttt
     2101 aaatggaatc caatagtttg gagctgatga atcttctggc aaaccatcat gaactactat
     2161 aaattcaggc actacagggt tatctaatac aacgaaacca gtaggaggtg gtaatggttt
     2221 taagttactt tctggaatct taggaggata agtaccatat agagtatgtt ctcctatgtc
     2281 aaaaatcaac tcactttgtc ttgagaaaga acgcttactt cttgttctag gagacatggg
     2341 aactccttgt cttgctacaa ttgtagctaa caactgcgtt ccttctatga caactgtctc
     2401 atatccatct gctatggctt ccactatata ctggctataa ggtctaacat cagatggctg
     2461 tagagagtaa tctacatctg gtgctggtaa tactaaacca gtcacttgac cagattcatt
     2521 acttcttagg ttttggtata aagttgatga gctttggcta ccattagaca ttgaatatat
     2581 gtttactact gcattttgaa taggtctatt gttagtagca tcaattatgc ttactgttaa
     2641 gaaaccatct tgcatcaaat caccctttct ttaaatgaat ttagttccct tatatttcaa
     2701 gatatgaata tagcttgaaa tttgttaaat aatatagata taaatttaaa tttttttgag
     2761 tatttgagta tatttgtatt taaataattt aaaataattt aatgtatatt ttagtatgta
     2821 tttaaaacac aaataagcta aaaataagta gatgatataa atcataaata aatcaaaaat
     2881 tttgaaagga aaataatcta tgaagctaga tttattttta ttagcaatag ttcctatatt
     2941 gatagggatg ttttggataa ggtctaaaga tagatattgt agagaacccc taatccatct
     3001 aattaaattt tttttaatag gtgcttttct aagtgttata attatacttt tagaaaattt
     3061 actgatgaag tttaatgtat ttgaagggta ttcagaacta atatatgtat catttgtagt
     3121 tgcaggactt gtggaagaag gtgtaaaagc tctgatactg attcctgccc taattaaaga
     3181 aaaacacttt actgaaaagt tagatggtat tatttactct gtatttttag ctttaggatt
     3241 tgcaacaatt gaaaatatgg tttatatttt ttctgaaagt agaaatctag cactgcaagt
     3301 tggtattaat agggcagtta tctctatacc agctcatgta atgtttgcaa ttactatggg
     3361 ctactatatt tctaagtata aatttgaggg aaataaaaat aaaagacgtg agtatttgtt
     3421 tatggcagtg ttaattccaa ttttattaca tggagttttt gatttcatat tgatgataga
     3481 atatagatgg gctattatat tacttatagt ttatgtaata attttgtgga agataaatct
     3541 tgataaactt gagaaatata tgaatcactc taaaaaagtg tttttcggta atcttaggaa
     3601 aaagaagaag aaataaaatt taaaggaaag acataaataa ttaaagtata ataaatgaca
     3661 cacaagtaaa gcagattgaa gggggattat agatttgaca gaattaccaa aggaattttt
     3721 agaagatatt gaagaaatac taaaagatga atatgatgat tttataaaaa gttatgagga
     3781 aagtaagact actgggctta gaataaatac tttaaaaatt gacaagaaaa aattatttga
     3841 tttggagttg tttgatttga atcaaatacc atgggcagaa gaaggttttt attatgatga
     3901 taaaattgac agacctggta gaaatccact gcatgaggct ggagcatact atttgcaaga
     3961 gccttctgca atgagtgtaa taccaaaggc agatattaaa gagggtgaaa aagttctcga
     4021 tatgtgtgct gctcctggtg gaaaatctac atatatacta tctaagctaa acgatacagg
     4081 tttattggtt tctaatgaaa ttaatgctag tagaattaag gctctagggg aaaatttaga
     4141 gcgatttgga gcgagaaatt gtattataac aaacaccgat tctaatagct taagaaagac
     4201 gtttaatggg tattttgata aggttattat agatgcacct tgttctgggc aaggtatgtt
     4261 taggaaggat gaggttgcca tatcagattg gagttatgca aaggtagtag aatgtcaatc
     4321 catccaaaaa gagattatca gagatggata taaaatgtta aagaaggatg gcattttaat
     4381 ttactcaact tgtacttttt caagggagga aaatgagcat gttataaatg agtttatcag
     4441 tgaatatcca catgcagaac ttattgaaat ggaaagatta tggcctcata agataaaggg
     4501 agaaggtcat tttgttgcca aaataaaaaa attagaagaa gaagaatgta atgtcaaaga
     4561 attaaaaata aaaaatttag ataaagaaat aaaggattat agggagtttg aaaagaaatt
     4621 tttaaaggtt gatttttata gtaataaaaa aaataaattt tatttaagag gagaaaattt
     4681 gtacctactt cctgaaatat atccagatac gaaaaaacta aaagtattga gatacggttt
     4741 gcatttaggg gttttaaaga agaatagatt tgagccttct catgcactat ctcattactt
     4801 aaaagttgaa gatgtaaaaa atgttgaaaa cttcagtgta cagagtgaat caattcttaa
     4861 atatttaaaa ggggatgttg tgaattctaa tgatagtaga ggatgggttt tggtttcggt
     4921 tgaaggaata cctttaggtt ggggaaaaga atcttctggt gtaattaaga atcattatcc
     4981 taaaggtctt agaaaagtat tttaaactat tagaaaaaag aagtataata tttatattgt
     5041 aggtttatgg tagattattt tgctgtagat tgacttaata tttaacacag aggagacgat
     5101 tttagtgggt gaagcagtta aaaaagaagt tgtagaatgg ataaaagtga ttgtcatagc
     5161 tcttgttttg gcatttgcaa taactcgttt tatagtgcca acaatagtca aaggagaatc
     5221 aatgtatcct acattagttg aacgtgatta tttgatagtt aacagaattg cgtacaaggt
     5281 aggagagcca aaatacaaag atataatagt atttaaaacc gacttaacag aggaaaatgg
     5341 aaagaaaaaa gatttagtaa aaagagttat cggggttcct ggtgaccatg taaaaataca
     5401 agactccaag gtatatgtaa atgataagtt gttagatgag acttcctata tacataataa
     5461 tcgtactgat ggagatattg atatcgtagt tccagaagga aaattatttg caatgggaga
     5521 taatagagaa aaaagtttag atagtagata cgatgaggtt ggattggtcg acgagcatac
     5581 cattttagga aaggttctag tcagattgta tccattttct aagataggaa ctattgacta
     5641 aacatataaa atttggagca atcgctgatt agctgaaatc accagaccat acacaatagt
     5701 tgtctatata aaaaagacaa caccttattt agtagactta tctaaaagat agtgtttaag
     5761 agacattatc aataaagata atataactat aaaggagaat ttgtgaatga atgaaactat
     5821 taaagaagag attgtagagt ggataaaaat aattattact gcactttttt ttgcatttat
     5881 tataactcgt tttataaaac caacactagt aaatggagaa tcaatgtacc cgacacttaa
     5941 atcacatgat tatttggtag caaataggat gacatataag ttatcagaac caaaatgtgg
     6001 agatataatg atatttaaga ctgatttatt acaagagaat ggaaggaaaa aagagcttgt
     6061 aaaaagggtt ataggtgttc ctggtgacca tctaaaaatt aaggattcta aggtttatat
     6121 aaatggtaag ttattaaatg aagtttcata tatacatgat aattatactg aaggcgatat
     6181 tgatatggtg attcctaagg gaaaagtatt tgcgatggga gacaatagag aagttagttt
     6241 agacagtaga tataaagaag tgggattggt agatgaagaa aatattaaag gaaaagttat
     6301 tttaagagta tttcctttta cagatatagg tatttttgag taggtgattt gtatggcagt
     6361 tggtatagcg tccttattgt ttaatattga ggaggcttta aacatttgtg agagtataaa
     6421 gcaaataact catttagaga ttggaattga caatatatct gaatgtagcg agttatgtaa
     6481 atataaagag agaatttcta agttaggatt atctataggg atacacttgc ctatggaatt
     6541 aaatacttgc gaaaacatag agtatatacg aaattcatgg ataaatttta ttgagaagat
     6601 agaatttgaa ctaaaaggat ttgatataag gtattttaac cttcatttgg gatatgttat
     6661 gacaaataga gttagaaaga atagagacaa atatttagga aatagtgtgg attttttaga
     6721 taaattaaat acaaattcat atgtatgtat agagaatact tattcaaaag gtggggattt
     6781 ttcaaatatt ggaaatatat cttatgattt tgaatatata tttaagagga ttaaaaattc
     6841 taagatttgt ttttgttacg atactggaca ctgtttaatt gatgaggatg cttatgtaaa
     6901 gaacttaaag gataaaataa gactgataca cttaagtgat aatgatggta tcaatgatac
     6961 acatgttggt ataggaagag gaattttatc tgaagagggg attaaagagg tcttaacatt
     7021 agatgcagaa tatttagtat tagaaataaa ttacgaagac attgaagata ctataagtaa
     7081 attgaataat attgtaagag agggttaaat acctctcttt atcttgttga tatgcctcat
     7141 taactaaaat ttatattcat caaaaggaag gagatgttga agtgaaaaaa ataaacttaa
     7201 aaatagaagg aaaagataag gaatattcat tagaagaaaa ttcccctgga atcagattgg
     7261 gagatatagc gaaagaattt tgtgatgagc ataagggtta tataacactt gcagttgtag
     7321 acaataaact aaaagagcta aattgtagag ttaagaagga ttgtgagata aattttttag
     7381 acactactaa tgaagatgga gagagagttt actttagagt catgtcattt atatttgtaa
     7441 tggcttgtag agaaatcttt tgggatagca gagttacaat agaacattca ctatcagatg
     7501 ggctatattg tgaagttcat atagatagaa agcttaaaga agcagatgtt gaaaatataa
     7561 aaaacaagat gaaagagata gtaaacaacg attatgttat agaaaaaatt gaagttacaa
     7621 gaaatgaagc tatttctata tttgaaaata atgaaatgta tgaaaaggca gagcttttga
     7681 aatacaaaga gtatgatagt gctaaagtat ataagtgtag aaactatata gaccattttt
     7741 atggttatat gttgccatca actggttata taaaatcatt tgatattaag ctttataatg
     7801 gaggcgttat aattttaggg cctagtgaag aagataagac tcttcctatg aagtttgtac
     7861 ctcaacctaa gctatcaagt gtatattatg aagcagaaga atggtcaaga cttatgggaa
     7921 ttgacaaggt agtatctctt aacaaaataa tagaaaacaa tgaatatgga gatataataa
     7981 ggacattcga agcattgcat gaaaagaagt tatcccaaat agcggatatg ataaaggatg
     8041 gcaataagag agtaatttta atagcagctc catcttcatc tggaaaaaca agttttgctc
     8101 acagattatc aatacatctt agagtaaata atttaaatcc tatttcaatt tctttagatg
     8161 attattttat agatagaaaa catactcctt tagatgaata tggaaattat gattttgaat
     8221 caatttatgc aatagattta gagaagttta acttagactt aaagaaactt ttagctggtg
     8281 aagaaattga tgatataaga ttcaatttta aagaaggtaa gagagaatac actggtaaaa
     8341 aaataaagat agattcaaat cagccaatta tattagaagg tatacatggg ctaaatccta
     8401 ttttaacatc atctatacca gatgaagata aatttaagat atacataagt gcccttactc
     8461 aaataaattt ggatgaccat aatcgaatcc ctactgctga tttgagaatg ataagaagaa
     8521 ttgttagaga ctataatttt agaggatata gtgctgataa tactatttta caatgggcat
     8581 cagttagaag aggagaaaag aagaatatat tcccgtacca agaggaggca gatgctatat
     8641 ttaactctgc ttgtgtatat gaattagcag ttttaaaacg atttgcgaaa cctttgttgg
     8701 aagagataaa ggaagataat cctgcacata ttgaagctac tagattacta aagtttttgc
     8761 agtattttgt tgaattggac gatacttctg acataccagg tatttcaatt cttagggaat
     8821 ttattggagg aagtaaaata gttgattaat atgtgtatta tagattgttt tatgaagatt
     8881 aaatggcata gcaagtagtg tgattggttt atgttaagta tattaagtgt aaagtggaga
     8941 attaaattga tacagatttt aaagtttgag ttgatatata aaaaggcttg ttttatactt
     9001 ttgtatgaag caagcttttt attttaaatt ttactttttt gtttaatatt ttgttagttt
     9061 atgtgataat ttt
//
LOCUS       DAELXX010000137         8968 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.139, whole genome shotgun sequence.
ACCESSION   DAELXX010000137 DAELXX010000000
VERSION     DAELXX010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8968
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.139"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(325..1221)
                     /locus_tag="KPH29_003249"
     CDS             complement(325..1221)
                     /locus_tag="KPH29_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG1468817.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDFLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASRVNLSAHHFCR
                     TFKQITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKRNYNI
                     SPTKYRNEHREVSNVILKEDMK"
     gene            1363..2790
                     /locus_tag="KPH29_003250"
     CDS             1363..2790
                     /locus_tag="KPH29_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG1468818.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIGKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFDWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFGFFLMSRLKESSALILFVIYISIISIGQAIFQPANNALIMSSCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVTLVCLIGAILIGFYLFKYNKNEQ"
     gene            complement(2987..5581)
                     /gene="clpB"
                     /locus_tag="KPH29_003251"
     CDS             complement(2987..5581)
                     /gene="clpB"
                     /locus_tag="KPH29_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBG1468819.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNDFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSDDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
     gene            complement(5618..6214)
                     /locus_tag="KPH29_003252"
     CDS             complement(5618..6214)
                     /locus_tag="KPH29_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468820.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDF
                     FNSDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            complement(6231..6635)
                     /locus_tag="KPH29_003253"
     CDS             complement(6231..6635)
                     /locus_tag="KPH29_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HBG1468821.1"
                     /translation="MDKTLKKDLNQEYTESKSLVMNKSKYMNICFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
     gene            7002..7376
                     /locus_tag="KPH29_003254"
     CDS             7002..7376
                     /locus_tag="KPH29_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG1468822.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            7373..8236
                     /locus_tag="KPH29_003255"
     CDS             7373..8236
                     /locus_tag="KPH29_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1468823.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRNPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            8236..8874
                     /locus_tag="KPH29_003256"
     CDS             8236..8874
                     /locus_tag="KPH29_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBG1468824.1"
                     /translation="MKGLILKDLLNLKGNVKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLLMILCFLVPTLA
                     LLVFKSILENISSPISIEIILNTLVYSLPFVAILLFVISYFISSKIYSKKEV"
BASE COUNT         3004 a         1319 c         1034 g         3611 t
ORIGIN      
        1 ttttctataa aaatcatact attttagctg tttacttgct tatttgatag ttttatttct
       61 ttgattaaat atctttcaga atctaagcca gatgtctcaa aaaaactgat ttataatata
      121 atcatttttt ctgctattct tatttttcca attttagttt catattaaaa tatacttttc
      181 atagacacta cattctttca tactcatttt cacatttaac tatacaagta gtcttactca
      241 tttatgtact atactttatt aatatttatt aagaaaaact caaagattta aaaatatata
      301 ttttctataa agtatattta tgaattattt catatcttct tttaaaataa cattactaac
      361 ttctctatgt tcatttctat acttagttgg agatatatta taattccttt taaataagcg
      421 actaaaataa tttatgctat caaagccaca tctcatagca atttctgtca tatttaaatc
      481 tgtttccttt aaataatata tagctttatc aagcctgatt ccatttataa aatcgattgt
      541 agtcttacct gttatttgct taaatgttct acaaaagtga tgtgcactca agttcacacg
      601 actagcaagt tcttttaatg taattttatc catatatttt tcttctatca agtcaaagat
      661 acattcaaat ttttctaaag aatactttct ttttataaat tcatcttcag aatatatttt
      721 atcaatatga cttcttaata agagtactat taattgatag atattagctt ttatagataa
      781 ctcatatcca acttctttat tgtgatattc atcaagtata gttgtaatac attttaaaat
      841 cagaaaatca ttttgaatta aattattaaa tacaatatga tttaaagcca atggtgctaa
      901 gtacttagtt tgtaacaaat caacttgatt actaaaaagg aatgtgggct caatacgaat
      961 actatagaat tttaaattat ctgataagct ttctaaataa tgtaattcat tactgtttgc
     1021 tattacaata tttccaggca ctaccttaaa attatccaat ccacattcaa gatatgctat
     1081 accatcaata aaataatata tttgtagatg ctcatgccaa tgtcttttaa agtttgctcc
     1141 ttttttaatg tcatccttta aaaaaatatc tataaagttt tcttgacctt tcctattaaa
     1201 atatgtattt ttatccttca ttattccccc ctcatcaaaa tagtgctaat tataagcaat
     1261 atagttattg tattcattat ttcatatcgc tattatataa aatgtaacta tcattgtaaa
     1321 cattaacata atgccagtca ttataataag gaggtaataa aaatgacaaa ctcttttaaa
     1381 aaagatgata atagaaaaaa aatcacagtt ttatttgtag tcattgccat gacttttatg
     1441 gcaacattag attcgagtat tataaatgtt gctctcccag ttcttgcaag caaactaaat
     1501 gtatctctag catctattga atgggtaata gcaagttact caattattat ttgttcaaca
     1561 ctattatttt ttggaagact tggagatatt attggtaaat caagagtatt ccaagtaggt
     1621 actattctct ttacatcagc ttcattacta tgtggattaa gtaattcttt aactttactt
     1681 atagtttgcc gctttattca aggaataggc gcttctgcct acatggcaaa caatcatgga
     1741 attatcacag aactatttcc aaaagaaagc agaggaaaag ccctagggat tctagttact
     1801 gcagttgcta ttggaaatat ggttggacca tctgtgggag gatttatttt gtcaatattt
     1861 gattggaatg ttatattttt tataaatatt ccaatcggtc ttattgtaat ctttttaaat
     1921 acaaaatttc ttcctaatag taaaaaatct tcagaaaata tggataaaac tggtgctata
     1981 cttcaatttt taggaacaac attatttttt agtgctctaa ttagtgctca acaaacagga
     2041 ttactaaatc catatatatt aattgcacta ttattatcaa ttatttttat tatattattt
     2101 ttgatattag aaaaaaaaca tcctcaaccg cttcttgatc ttgaaatttt tagaaatttt
     2161 aaattttcat tgaatttaat ttgtgctctt acatcattca tttgtattgc atcatcaagt
     2221 attttaattc cattttactt acaaagtact atgaaacttc cccctattca agcaggttta
     2281 tttatgatac tatcaccttt gattttagcc attttttcac ctatatttgg gaatatatca
     2341 gataaaatca agtcagaaaa aattatttta attggtcttc tcgttatgag ttttggattc
     2401 tttctaatgt caagattgaa agaaagttct gcccttattt tatttgtaat ttatatatcg
     2461 ataatatcaa taggacaagc tatatttcaa cctgctaaca atgctttgat aatgtcaagc
     2521 tgttctagaa gtaagctagg agttgtagga agcataaatt cccttgtaag aaatttagga
     2581 caagttatag gtattactat ttctacaaca cttttatata attttatgag tattaaagct
     2641 ggatataggg tcaatgatta tgtgattaat aatgataaaa tatttgtatt tggaatgaga
     2701 aatgtttata ttattgttac acttgtttgc ttaataggtg caatattaat tggcttttat
     2761 ttatttaaat ataataaaaa tgaacagtag ttttttataa ttatattcaa attatgatta
     2821 tattataaga aacttactaa aatacttcct ttgagttaaa tttaatttgg acatattgac
     2881 ttaaaaatgt tttattaatt tgataaaata tttcttttcc tatagtttat aaataaaaat
     2941 cagctgagca aaatatttag tatattatgc tcaactgatt cttttattat ttaactaaaa
     3001 cttcaatatt ttcatctttt ccatctataa ctatagtaca tccattatac acatctccag
     3061 caataagttt ctttgcaatt atagtttcta atgtatttcc tatatatctc ttaagaggtc
     3121 ttgctccata tacagggtca tatccttctc tcaccataac atcttttgct gaatttgtaa
     3181 cttcaatagt aatatctttt tcttttaatc tattttttaa atctttcata aatatatcta
     3241 taattttctt gatattttct tggtctaaag gcttaaacat tataatatca tctactctgt
     3301 ttaaaaactc aggtttaaat ctatgcttca tttcattcat tactaaattg ttagtagtct
     3361 ctgttatatt tcctcctgct tctaatagat aattacttcc tatattagaa gtcattatta
     3421 taagagtatt tttaaagtct actgtcttac ctttattatc agtaagtcta ccatcatcta
     3481 atatctgtaa gaacatgtta aatacatcct catgtgcttt ttctatctca tcaaatagta
     3541 ttacagaata aggagcacgt ctaactgctt ctgtaagttg accaccctct tcatatccca
     3601 cgtaccctgg aggaggtcca actagtcttg atacagcatg tttttccatg tattcagaca
     3661 tatctattct tattatatta tcgtcactat cgaataaatt tctagcaaga gtttttgcaa
     3721 gttcagtttt accaacacct gtagggccta agaaaataaa tgaaccaatt ggttttcttt
     3781 catcttttaa tcctgctctt gcacgtatta ctgcatttga aactgctgta actgcttcat
     3841 cttgtccaat aactctttta tgaagttcat cttctagttt aagtaatttt tctctttctc
     3901 cttcaactag tttagttact ggtatacctg tccactttga tacaatgcta gatatttctt
     3961 cttcagtaac ttcttctttt aaaagagaat tttcagaacc atcattcata ttttcttcgt
     4021 actgctttat ttgttcttct aacttaggta tttcaccata tttaacctct gctgctttgt
     4081 tgaaatcata ttctctttca tacttttcag ctttaccttt agcttcgtct aattgagcct
     4141 ttaagttttt aatatctaat atttgactct tttctttttc atattttgct gtcatttcat
     4201 catttttaga ttttaactct gctatttctt tttgaatatc atccaatctt gatttgcttt
     4261 tatcatcatt ttctttaagt aatgcttctc tttcagtttc taatgtaaat aactttcttc
     4321 taacaacatc taattctgtt ggcaatgaat ctatttcact acgaatcata gctccagctt
     4381 catcaattaa atcaattgct ttatctggta aaaatctatc ttgtatatat ctatgagaaa
     4441 gttttgctgc tgctacaata gcattatcat gtattcttac tccatgatgt atctcaaatc
     4501 tttcttttaa acctctaagt atagatattg tgtcttcaac agtaggttct tctgcaatta
     4561 caggctgaaa acgtctttca agagccttat ctttttctat atactttcta tattcatcaa
     4621 aagtagttgc accaatacag ttcaattcac ctcttgcaag cataggttta ataagattac
     4681 ctgcatccat tgaaccttct gttttaccag ctccaacgat agtatgaatc tcatctataa
     4741 acagtataat ctttccatca gaactttgaa cttcttttaa aactgccttt aatctttctt
     4801 caaattcacc tctatatttt gcaccagcaa ttaaagctcc catatcaagt gagaatacaa
     4861 tcttatcttt taaaccttct gggacatctc ctcttactat tctctctgct aacccttcta
     4921 caattgcagt tttacctaca cctggctcac caatcaatac aggattattt tttgttcttc
     4981 ttgataaaat tcttataatc cttcttattt cttcatctct cccaattaca gggtctaatt
     5041 tatttttctt ggctaaatct actaagtttg ttccatatct agcaagtgca tcatatgtgc
     5101 cttctgggtc ttgtgtttct actctttgac tacctctcac ttgtgataaa atatttaaaa
     5161 agtcattctt atttatatta tattgtttta atattttacc aacagctgat tttgattctg
     5221 tttccatcat tgcaagcata acatgctcta cacttatata tgaatcttta aattctttag
     5281 aaatactctc tgcctttata agcacctcat ttatttttct agtagctgat actccttgtg
     5341 agtcagcacc ttctccatat acttttggaa gcttatcaat ttcaaagttt acagtatttc
     5401 tcaaagaatc aattgatata ttcatttttt cgattatatt tggtatcaat ccatcctctt
     5461 gattaataag tgctgagaaa agatgaatta catctacttg ttggttatga ttttttactg
     5521 cttcgttata agcctcattt aaactttttt gaactcttaa agtcattttt tctacgtcca
     5581 tataaatccc tccattttaa tattatatcc aaataaatta aattctctca tttataatta
     5641 ttgattttct aagctctaaa agactatcaa gtaaattctc gtttttttgt atatcgaaac
     5701 tcaatgttaa attaaatgtt ccatctgaat taaaaaaatc gaatacttct tttgattttg
     5761 ctattaaata caagtcttca tctataagtc ttttttcacc tattaataaa tactgacatt
     5821 tatctataaa actcttttta aaatcaatag tgtatagatt ttctaaaata ttatattgat
     5881 aaatagcaat attattaaac acttttaata tgtttgaatt tacaaaattg taaatttgat
     5941 tattgttata cagtgatatt tgttcgtata tatattccct aaataaagga tgaatgtttt
     6001 gcttaagttt taaagtaact tcagctggta tttcaaaata aagcttccag tacttagagt
     6061 tatttttttc taaaataaaa gctactttgt tcaaactggt agaactaatc tcctttagtg
     6121 tacaattaaa attatagtgt ctcaaactag ttttttcatt ctcaaataca tagtcaaacc
     6181 actgtagaac atttctatca agttttaaac tcatataaaa accttcttcc ttatttacta
     6241 accttattag gtctttttat ctgattatat tctgctaata aactttcttt atcagctcta
     6301 ctacctttat atgctatatg tggatttatc atatactcac catttttcac tctttgtaaa
     6361 aaatccattt ttttcaactc cgtaaacaat gaagataaag aagtttttgg atattcaaat
     6421 tcacaagata attcatataa tgtttttcta atcttattat ttttatccat agagtttatt
     6481 aattttaatg gaattaaatc cctcttagta tacattcctt ctactacttt aatataattc
     6541 ttaaaacaaa tattcatata cttactttta ttcataacta aacttttgct ctctgtatat
     6601 tcctgattta aatccttttt taatgtctta tccaaattaa atatctcttc tcactctcct
     6661 tatctctaat tgataatata aatatattac aaaaaaatat gtatgtcaaa actgaaaatg
     6721 caaaaagttt accttataag taaacttttg tttagctata agctaaactt tattattttt
     6781 ttaatttagt atttccaatt tattggcagg atttttgaat aatatttata aattttttaa
     6841 aaaaatttaa atctatttaa tatggcagcc tttcataaaa tacattacat ttattaaatg
     6901 tataatattg gtaattagtt ttttctaaaa tcagttgaca tattatatat atatttatat
     6961 aatgtgtata tactatatat acaatttaag gaggtgactt attgaatata gttattagta
     7021 attcgagtgg gaagccaata tatgaacaga ttactacaca aattaaaagt atgattataa
     7081 gcggagagtt accagaaggc tcagctcttc caagtatgag actattggca aaggaactta
     7141 gaataagtgt aattactaca aaacgagcat atagcgattt agaacgagac ggatttattg
     7201 agacagttac tggtaaaggc agttttgtat ctagtaaaaa catggatttt gtcaaagaag
     7261 aacaatttcg tttaattgaa gaatacctac aaaaagcagt tgatacagca aaaagtagtg
     7321 atattacata tgacgaatta aaagatattt tatttttatt atataaagga gaatgataag
     7381 tatggatgca ataactataa aaaatttaaa taaaacttat aaagatttta gcctacaaga
     7441 tatatctttt tcagtaccaa aagggagtgt aatgggattt gtaggtgaaa atggtgcagg
     7501 aaaaacaact acattaaaag caattttaaa tttaatatct tatgatagtg gaaatataga
     7561 aatatttggt cttgatagta aaaaaaatga aaaagaaatt aaagagcaaa ttggggttgt
     7621 atttgaggga agcaactttc atgaaaattt aaacactgac catgtttcaa aaatcatgag
     7681 taaaatctat aaaaattgga atgacacatt atttaaagac tacttaaaaa aattgagagt
     7741 accagacaat aaattaatta aggagttttc aaaggggaat aagatgaaac ttagtattgc
     7801 tgtagcctta tctcataaac caaaattatt aatactagat gaggcaacta gttcacttga
     7861 ccctatcgtt cgtgaagaaa tactagatat atttttagat tttatacaag atgaagaaca
     7921 ctctattatc ttatcttcac atattacaag tgatttagat aagatagcag actatattac
     7981 atttatacat aaaggaaaaa ttgtatttag tgaaaataag gatgaattaa ttgatactat
     8041 gggagtttta aaatgtaaac ctagtgattt tgataacctt tcaagagagg attattcata
     8101 ttatcgtaaa agccaatttg gatatgaagt acttcttaaa gataaacata gatttatatc
     8161 aagaaatcca aattgtattg ttgataatac gtctatagaa gaaattatgt tattttatgt
     8221 gagaggtgat aaataatgaa aggtttaatt ttaaaagatt tacttaattt gaaaggaaat
     8281 gtaaagttta tacttttatt catcattatg tttgggttta tgtcttcttt aggagatgga
     8341 aatgttaata actttatagg tgttattata gttttatgta ctacaatgat tgtttcaaca
     8401 ttttcatatg atgatttaaa taaatgggat agttatgtac ttacaatgcc tattaatcgc
     8461 aatgatattg tattaagtaa atatttaact atgttaatat ttagctttat aggtgttctt
     8521 gtttcactta ttgttagtgt aactattggt tattttaaaa atacacttat attaaatgaa
     8581 acactactta ttaatgcact aatcttatca atttcagtat gttttggtag ccttattcta
     8641 ccacttattt ataaatttgg tacagaaaga gctcgtttat taatgatttt atgcttttta
     8701 gttccaacac tagctttatt agtttttaaa agtattttag aaaatattag ttcacctatt
     8761 tctatagaaa ttatacttaa tacactagta tattctcttc catttgttgc tattttacta
     8821 tttgtgattt catattttat atcatcaaaa atctatagta aaaaagaagt ataaaatata
     8881 ctatacatgt ctctgtattt aagtatttta aataaaatac tatcaagttt attttggtag
     8941 tattttattt ttttattatt aatatcta
//
LOCUS       DAELXX010000138         8785 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.140, whole genome shotgun sequence.
ACCESSION   DAELXX010000138 DAELXX010000000
VERSION     DAELXX010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8785
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.140"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(157..273)
                     /locus_tag="KPH29_003257"
                     /pseudo
     CDS             complement(157..273)
                     /locus_tag="KPH29_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
     gene            complement(361..1455)
                     /locus_tag="KPH29_003258"
     CDS             complement(361..1455)
                     /locus_tag="KPH29_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HBG1468825.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVIKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMKMGITSHDEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGGTCDNRSRFASEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKDEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADSNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            complement(1457..1903)
                     /locus_tag="KPH29_003259"
     CDS             complement(1457..1903)
                     /locus_tag="KPH29_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG1468826.1"
                     /translation="MDFKNLQYESLSYIICNLQKNFKLYCEKCLKPYKLTNGLYFYLI
                     YINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSRAFQLRVTDKGE
                     EVLGDIKNIFSKWDNHIKKEFSDTEYKELVKNLHVVKDIKTAVEEG"
     gene            2209..3399
                     /locus_tag="KPH29_003260"
     CDS             2209..3399
                     /locus_tag="KPH29_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG1468827.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
     gene            3569..4531
                     /locus_tag="KPH29_003261"
     CDS             3569..4531
                     /locus_tag="KPH29_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBG1468828.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHIFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVLSPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(4649..5311)
                     /gene="zmp1"
                     /locus_tag="KPH29_003262"
     CDS             complement(4649..5311)
                     /gene="zmp1"
                     /locus_tag="KPH29_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HBG1468829.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHAYSTTIQQNKDTLSQIVVF
                     PTGNYNKNEANAMVNRLANIDGKYLNVLKQDNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDAINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYPEEFFAEAFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     regulatory      complement(5411..5496)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(5682..8636)
                     /locus_tag="KPH29_003263"
     CDS             complement(5682..8636)
                     /locus_tag="KPH29_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBG1468830.1"
                     /translation="MKKGNKKALLISLIMILSMVVSTIYPTVSYASELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEKDKATDNKKPEQTPEEDNSTDNKKPEQTSEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQTPEGEKSIDNKKPEQIPEEDKSTDNKKSEQALEDEKPL
                     DNKNTEKTPEEDNLLEDENLLKVLEEELNEDYGFVVKINNNTIETESMKKISFNLTYT
                     PTSKGIQAGDSITFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGK
                     IGLEAYVKKVDEDTNAKIHIETTGKIESGSGDIDVEIKPGDKTDVPDAKGTLKKLVEG
                     RKSTTVFMPVKNKDINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEV
                     SDFNIEQSKNSISVNFGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQED
                     DATVSIFDRGDNYLLTKGHSGATNITQVGQVINYQISINDDKSPISNVVITDNIPEGM
                     RLTTSGEDGHDFRVVEVPMNGSWTPWSKEKIANNISYKVEEKRNESGQVDKVITGFTI
                     NLSKEEVESKFFIAYTLKVISIEDSYINRAVLDANNSEIDKNDEINFKKNSGLISAKK
                     EVDKKVLNSSDNQIVKYKINMSTYGVYDAGQVNLLDEVNPSLKISNIKYSDNLELKKE
                     AGDGKNTIRLVNKYEFKQIKEGEPVQSWVTFDADFTNVKVGETIQNVAQINGSSPPGV
                     ETTKQGYAFEAKKVDALDKNVLSGAKFNLEDAFGNIVVKDLVSDEDGVIQSSVKKPGI
                     YYLVEVMAPSGYEKLKDKIKVEIGNKDIGRIVDIGNIENIRQENPPVNPPVPPNTDEP
                     IVNPPVPPSTDKPRKPSNPSDTEDTIVINPPVPPSEDIINPPIPEILNPPVPPSEEVI
                     ETPVKQIVPIPEVVKPSVSEEKNNKAKDDTLVNPPVPPKTGDSTTIIGEILLVIGAIV
                     GLVVLRRNKNTN"
BASE COUNT         2705 a         1419 c         1065 g         3596 t
ORIGIN      
        1 ccttatgttt tagtttgatt agctttaggg gtttccagca attcaagtag tattggatag
       61 gttctatcct atctctacat ataggtttcc ctatatgctg actattttag ctactaactc
      121 atgactaaag tcacaagtgt gcgtagctat tttttaatca actaattcat ttacatcttt
      181 aatgtctttt tcttcttgaa tttcatcata tatagcataa gcaatctttt tagtatttcc
      241 agttaaactt gaatataaaa ctaaactttt catgatgtct cctcctcttt atctaaatac
      301 ttatctaaat caccaaaaat atattaacct taacaaatta tttattttta taaagatatt
      361 ctaatctagt atacatccaa actgagagcg tgtttgtaaa gcatctgcac acttcttatt
      421 acaataacag cactttttaa tctcatctac tcttccatct tttactttct gtaaccaatt
      481 actatctgct agtaattgtc ttgacataga aaccatatct actctattat ttgaaataat
      541 actttctata aaatctgggc tagacaattt tccaacccca catactggaa gatttgtgtg
      601 tttctttact tcatcagcta aatataaaaa acagccttca tctttaaagt aaggatgatt
      661 attggttggt atagtatctt ctaattttga atgatttgca agtgttacat gaaaactgtt
      721 tacacctagg gattcaaatt ttttaacaaa atactcaact tccgaaagta aaactcctgc
      781 atttccataa tgaggatttt cttgtcttat tgcaaattta tagtctattg gaatgtcctt
      841 aacttcatct cggatactac taattatttc gcttgcaaat ctacttctat tatcacacgt
      901 tccaccatat tcatcatttc tattattaaa tatacttgat gaaaaacttc ctacaagcct
      961 atcaccatga atttgaatca tgtcaaaacc tgcttttttt gcattcaatg ctgttacttt
     1021 aaataaattt ataatatttt taatttcttc tttgggcata tttgttatgt aatcttttac
     1081 tccgtcattc attatttttt ttatctcatc atgtgaagta attcccatct tcatagtatc
     1141 tttaattaaa ttaacatcat aatctgaaca aaatagttgt gctgatacct ttgcaccaga
     1201 attgtgtatt tcatctacaa ttttcttgta tttaatgact ccatcttcat ccgataggct
     1261 tatagctacc ttatgaaaac taggtctaat agatacatct cctagagtaa tcagagctac
     1321 tcctgactta gcaatatcag aaaacttttt aattttttct tccaatttaa gacccatgct
     1381 tgtaggtgca aaagatactc tattttttaa ttccaaattt ccaattttaa ttggttttaa
     1441 taaattacaa tacatcctat ccttcctcca cagctgtttt tatatccttt accacatgta
     1501 aattctttac cagttcttta tattctgtat ctgagaactc tttttttatg tggttatccc
     1561 actttgaaaa tatgtttttt atatccccta atacttcctc acccttatct gttactctca
     1621 actgaaaagc tctactatca ttagggtttt ggattttttc tatgtatcca tcttgttcta
     1681 acctagatat agttcttgtt gtatgagctt tatcaacttc aaattctgta gaaacatcat
     1741 tcaaagaaca attcctattt ttattaatat aaattaaata gaaatataga ccattcgtaa
     1801 gtttatatgg ttttaaacat ttttcacaat aaagtttaaa gtttttttgc aagttacaaa
     1861 taatatatga caaactttca tactgtaaat ttttaaaatc catcttaaat ttcccttcta
     1921 taattataat atgtttgact atatcaacta tattatatta aacaattaat atagtcaaga
     1981 atattttatt ctctaaatct atgttttatt aattctttaa aaaactatta tataaaataa
     2041 tcatgataaa attagaaatt ggaattttta atatgcactg tacataaata tattttatat
     2101 aaaagtttat attttcaaac aagtaatttt tcgaaaattg tctaaattca gtaaaaatat
     2161 ggtaatatat acataagcaa tataaatcta taaaaaggag tgttacatat gatatctaat
     2221 aaaatgcaaa ctttagttgc aaatagttct gttataagag ctatgtttga agaaggaaag
     2281 aaactttctg atatttatgg tgaggaaaat gtttttgatt ttagtatagg aaatccaagt
     2341 gttgaacctc cagagactat taaagcagtt ataaatgata ttttaaatga agaatctcca
     2401 aatttagttc atggatatat gaacaactca ggatatgagg atgtaagaga tgctattgct
     2461 gaacatataa ataaaaaaga tggattaaat cttacaagag aaaatttaat tatgacttgt
     2521 ggtgctgctg gtggtctaaa tattatttta aaaactcttt taaatcctgg tgatgaagtt
     2581 attgcttttg caccgtattt tggtgagtat aaaaattata cagaaaacta tgatggaaaa
     2641 ttaatagaag ttcctacaaa tatagagact tttgaaccag atttagatgc tctaaaaaat
     2701 gctattactc caaaaacaag agctttaata ataaacactc caaataatcc tactggtgtc
     2761 atatattctg aagaattatt aaaaaatctt ggagaattat tggattcaaa acaaaaagaa
     2821 tttaatacaa gcatatatct aatttctgat gagccatata gagaaataat ttatgatggt
     2881 gccaaagttc cttgtgtact aaaatactat agaaattcat ttatagggta ctcttatagt
     2941 aaatcattat ctctacctgg tgaacgtata ggatatattg ttgctaatgg gcaaatggat
     3001 gattttgatg atgttatgtc ttcattaaat gttgcaaata gaatacttgg atttgtaaat
     3061 gctccttctc tttttcaaag ggttattgca agaagtttag atgcagaagt tgatgtaaat
     3121 atttataaaa agaattttga ccttctatat aatagtttaa tcgatatggg atattcttgt
     3181 gttaaaccta atggtacatt ctatttgttc ccaaaagctc caattgaaga tgataaaaaa
     3241 ttctgcaatg atgcaaaaca atttaattta ttattagtac caggttcttc ttttggttgt
     3301 ccaggtcatt ttagagtatc atattgtgta tcttatgata aagttaaaag ttctttacca
     3361 gcatttgaaa aattagccaa attatataat ttaaaataac taaatcaaag taattcaaat
     3421 aattaactac cttagtatag aatacttaca ttctatatta aggtagttta taattttatg
     3481 tactatattt tatacatacc atattattcc aatatgcttg aatattctta tctatagtac
     3541 taatataaat tacaaggagg tgtttgactt gaaaaaaggt tatgtttata taatactgac
     3601 taccttattt tttagttcaa tggaaattag tctaaaaact gtcactaatg actttaatcc
     3661 cttacaaata actttatcaa gattttttgt tggcggttta gtcttactac cttttgctgt
     3721 aaaaagatta agagctctta gtctttctat tacaaaatca gacttaaaat actttgcatt
     3781 tttaggtttt atgtgtgtag tagtaagtat gtcattgtat caactggctg ttctaaacac
     3841 aaaggcatct gtagttgctg ttttgttcag ttgtaatcca gtctttgtaa tgcttttagc
     3901 ttacttaatt ttgaaggaaa aaatatatag gcacaatgta atttcactta tattagaagt
     3961 tcttggaatt atagttataa taaatccatt gcatacaaaa ctaactctta gtggaatatt
     4021 cttaacatta tcttctgcta ttattttttc aatgtatact gtatttggta aaagaaaaac
     4081 cttaaaattt ggtggaatag ttgtaacttg ctttagcttt atatttggta gtttggaaat
     4141 gttgatattg gttttactca ctaaaattaa ttttatcgca gatatattaa ataaaaataa
     4201 acttcatata tttgcaaata tccctatttt tagtggatat accttacaca atatgcccat
     4261 tatgatttat gtattcgtat ttgttacagg tgttggttat gcactttact ttatggcaat
     4321 ggaagctaca tctactagtc ttacatcttt agtattcttc tttaaaccag tactatctcc
     4381 aatactagca ttattaatac ttaaagaaat tatccctata aacatggtta taggaatttt
     4441 attgatagta attggttcaa taatttctat tatacctacg ataattactc aaatgcataa
     4501 taaacacgtt gaagccttgt ataaggagta gtaattgata atctatcaag ttatacataa
     4561 gacaaaattt attagattta aacgcaatat aaaagagatg ctattataat agcatctctt
     4621 ttatatttat tttaaattta ttaaagctct atttagctag gttttgcaaa aagctataag
     4681 tttgtggaca agctgatttt aacttactat ttgtatcttg atttaaatag taatatgcaa
     4741 atgcttctgc aaaaaattct tctggatata cacctaagta gtttacatta cctaaagaac
     4801 gaccttcttt agcaaatatt tgtttgaatt gtgctgattt agatatatca tttaatacta
     4861 tatggtctat tgcatgtgct gtttcatgta attctaaatt tattgcatca tgccctttac
     4921 ctttgttact aaatcctatt ctaagagcta ctgtacttcc acctaagcct ggaacatcat
     4981 cccaagtttt tccagtacct tcccatccct taggaactac accttttaaa taagcatatt
     5041 ctttttcatc tgttaacttt ccacttaata attttatttt taagttatct tgctttaata
     5101 catttaaata ctttccatct atattagcta atctatttac catagcatta gcttcgtttt
     5161 tattgtaatt tccagttgga aaaactacta tttgactaag tgtgtcttta ttttgttgta
     5221 tagtagtact atatgcatgt gcacttactg gaactgaact tattaagaat attgatatta
     5281 tagctattaa taatttttta cttggtctca tattattccc tcctttgagt taggccggga
     5341 gtaataaatt tcaaaataaa aaagataccc aagagtatct ttgtatatac aaaaagaaat
     5401 ttttactctt ttcggcaact ggctggcatg gtttactaaa ctttagcccc tatagctttg
     5461 cgtgactagg tttccctagt tttgcctcat ttaattttat atctatatta tagcttatat
     5521 ctttttaatt gtctatatcc ctttggaact tttttctaaa aagttattat ttttttacat
     5581 aaatcttcaa aaaaaaatat cttctaacaa tattttcatt attaaatatt gttttagaag
     5641 atatttttta atataactta tattttttat aatgttaggg tctaatttgt atttttattt
     5701 cttcttaata cgacaagtcc tacaatagct cctataacta aaagtatttc tccaataatt
     5761 gtagtactat cccctgtttt tggtggtact ggtggattta ccaaagtatc atctttagct
     5821 ttattatttt tttcttctga cactgatggc tttaccactt ctggtattgg aacaatctgt
     5881 ttaacaggag tttctatcac ctcttctgat ggtggtactg gtggattcaa tatctctggt
     5941 attggaggat ttattatatc ttctgacggt ggtactggtg gatttataac tatagtatcc
     6001 tctgtatcag atggatttga tggtttcctt ggtttatctg tgcttggtgg cactggtgga
     6061 tttactattg gttcatctgt atttggcggt acaggtggat ttactggtgg attttcttgt
     6121 cttatgtttt ctatatttcc gatatctact attctaccaa tatctttatt accaatttca
     6181 accttaattt tatccttcag tttttcatat ccacttggag ccatcacttc tactaaataa
     6241 tatattcctg gtttttttac actagattga attacaccat cttcatcact aactaaatcc
     6301 ttaactacta tatttccaaa tgcatcttct aaattgaact tagcacctga aagtacattt
     6361 ttatctaaag catccacttt tttagcttca aaagcataac cttgcttagt agtctctact
     6421 cccggaggac ttgaaccatt aatctgagcc acattttgta tagtctcccc aacttttaca
     6481 tttgtgaagt ctgcatcaaa tgtaacccag ctttgcacag gttccccttc ttttatctgt
     6541 ttaaattcgt atttatttac taatcttatt gtattttttc catctccagc ttctttttta
     6601 agttcaagat tatcagaata ttttatatta gatattttta gagatgggtt tacttcatcc
     6661 aaaagattta cttgtcctgc atcataaacc ccataagttg acatattgat tttgtattta
     6721 acaatctggt tatcactact atttaaaacc tttttatcaa cttctttttt tgcgcttatt
     6781 aatccagaat ttttcttaaa gtttatctca tcatttttat caatttcact attatttgca
     6841 tcaaggacag ctctatttat ataactatct tcaatactaa ttacctttaa tgtataagct
     6901 ataaaaaact ttgattctac ttcttcttta ctgagattaa ttgtaaatcc agttataact
     6961 ttatctactt gcccactttc atttcttttt tcttctactt tatatgaaat attattggca
     7021 attttttctt tactccaagg agtccagctc ccattcattg gaacttctac aactctaaaa
     7081 tcatgcccat cctctcctga agtagtcaat ctcatacctt ctggtatatt atctgttata
     7141 acaacattac ttattggtga tttatcatca tttattgaaa tctgatagtt tattacttga
     7201 ccaacttgag taatatttgt tgctcctgaa tgtcctttag taagaagata attatctcct
     7261 ctatcaaata tacttacagt agcatcatct tcttgtatct tcttaccatc agattcaatc
     7321 ctagctacat tcttatattt attaccatgt tttgcatttt tgtcactgat tctagcctta
     7381 tacttaacag tatacgattt atctatattt ccaaaattaa ctgaaataga atttttactc
     7441 tgttcaatat taaaatcact aacttctttt ccatcacttg ttactacact tacagaacca
     7501 tttatcaatg tcaatccttc aggaagctca tcatacaaaa taatatcttt taactcttcc
     7561 tgcttttcat ttacttgtat actgtaattt atatctttat ttttaactgg cataaataca
     7621 gtagttgatt ttcttccttc aacaagcttt ttaagtgttc ctttagcatc tggaacatcc
     7681 gttttatctc caggttttat ttctacatct atatctccac ttccactttc tatttttcca
     7741 gtggtttcaa tatgtatttt tgcattagta tcttcatcaa cctttttaac atatgcttcc
     7801 aaacctattt taccttgcat tacactttgg ccattaggta attctctaaa tgtaagtgta
     7861 tactctccat tactttctgt tttatcaaag cattcacttg tataatctaa attaacttta
     7921 ttgaatacat ctggcacctt aaaagttatt gaatctcctg cttgtattcc ttttgatgtt
     7981 ggagtataag ttaaattaaa tgatattttt ttcattgatt ccgtttctat tgtattgtta
     8041 ttaattttta caacaaatcc atagtcttca ttaagttctt cttctaaaac tttcaataaa
     8101 ttttcatctt ctaatagatt atcttcctct ggagtttttt ctgtattttt attgtctagt
     8161 ggtttttcat cttctaaagc ttgttctgac tttttattat ctgttgactt atcttcttct
     8221 ggtatttgct ctggcttttt attatctatt gacttttctc cttctggagt ttgctctggc
     8281 tttttattat ctattggctt ttctccttct ggagtttgct ctggcttttt attatctgtt
     8341 gacttatctt cttctgaagt ctgttctggt tttttattat ctgttgaatt atcttcctct
     8401 ggggtctgtt ctggtttttt attatctgtt gccttatctt tctctggagt ctgttctggt
     8461 tttttattgt cgctttcctt ctcctctcca gttgatgaat tagttgaacc actttgaatc
     8521 tgactattct ctcctaattc tgaagcataa gatacagttg gatatattgt agacaccacc
     8581 atacttaaaa tcataattaa actaattaat aatgcctttt tatttccttt cttcatataa
     8641 attttccccc tctaggttat ctatagttta taatcttcca ttaatatcgt atataaatta
     8701 atttttacta aaaaattata taaaaaaagc tatgaaaata aataaattta tattcatagc
     8761 cggttgcacc actgactcag tatat
//
LOCUS       DAELXX010000139         8652 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.141, whole genome shotgun sequence.
ACCESSION   DAELXX010000139 DAELXX010000000
VERSION     DAELXX010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8652
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.141"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     repeat_region   70..296
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=70..98
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(957..3050)
                     /locus_tag="KPH29_003264"
     CDS             complement(957..3050)
                     /locus_tag="KPH29_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG1468831.1"
                     /translation="MNKRNFEVILNQLQINIYVTNIHTNEIIFMNKKMKEEYNILDPE
                     GKVCWQVLYPEQNSTCSFCKVLELLKNDKKGVLIKWYEKCNKLNRVFENYDSLITWQD
                     GTVVHMHQSIDIANSTILNKPIKINEFNEISNTKEGKGVFNFSRDNFDYNSTLLYDAL
                     IRGTDEYIYICNMKTGVFRYSPSQVELFDLPGEIVENPLIYWKKIVHPEDWNRFYKSN
                     TEIGKNQMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKID
                     SLTQLLNYHEFMSVFEDKISNPMIEKLCIVLLNIDDFKNVNEMYDRDFGDNIIKTLAQ
                     SIQSILPDNAELYKLDGDEMGILVDNVEENEILTLYNQIQNMIIHLQLWRKYGLNITI
                     SAGCVIYPKHGDTVKELYKCASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLK
                     ASINDDFRGFSLRFQPQVDTQSHKIIGVEVLLRWTNDKCKAISPLEFIPILEENDMIN
                     IVGAWVLRMALRTFRKWIDYYPFFKVSVNVSAVQILEDTFIEDIVKIIDDENFPYQNL
                     VLELTESHTVQNMSILQFKFKALQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRV
                     FVKDILKSKFDATFIHFIVAICHDVGIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGK
                     PQTATEIFDLLKLDN"
     gene            3703..4626
                     /locus_tag="KPH29_003265"
     CDS             3703..4626
                     /locus_tag="KPH29_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HBG1468832.1"
                     /translation="MGSGKVGTSLANYFLSKGYCVSGFYGRNQLSLLESINLTKTKIY
                     SNLRDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSKKAG
                     ALIYSIHPIFAFSNKNTNIKKMKDICFSIEGDNEQDDLVIQNFIDGLGNQFFIRDKDT
                     SSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNGFTK
                     ALTGPVSRGDITPIRKHLSVLNKKDEDIYKILSLNLLKIIALGNDNSLKGTKNITIEN
                     AIENLISKSEKYKEIYKLLGGKNDEKYHTDF"
     gene            4601..5428
                     /gene="panB"
                     /locus_tag="KPH29_003266"
     CDS             4601..5428
                     /gene="panB"
                     /locus_tag="KPH29_003266"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBG1468833.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGAKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIADMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGVFGDFVPKFVKQYANIGDIMKDSIKNYILEVNTGAFPQEKHSFSINESELEKLY"
     gene            5447..6295
                     /locus_tag="KPH29_003267"
     CDS             5447..6295
                     /locus_tag="KPH29_003267"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBG1468834.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAV
                     KENDKVVVSVFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNINTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PKNIKELITAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFELNI"
     gene            6706..6888
                     /locus_tag="KPH29_003268"
     CDS             6706..6888
                     /locus_tag="KPH29_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468835.1"
                     /translation="MFKDKIDECVHIMTAYIASLKEYYSFIETQIGDFIKKYGEDVVE
                     LCLHRVMILLCECGLA"
     gene            7720..8634
                     /locus_tag="KPH29_003269"
     CDS             7720..8634
                     /locus_tag="KPH29_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HBG1468836.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKDITT
                     RELNLQSGVNPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRSYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
BASE COUNT         3283 a         1169 c         1062 g         3138 t
ORIGIN      
        1 aaatttatat tccgtatagt tagtataaaa cgtaagtaat ataaatttat gtaaaacaac
       61 aaaagatgga tttacattcc atatagttaa tataaaacgt tttaatgaat taacagaaga
      121 tgaaagtttt atatgtattt acattccata tagttaatat aaaaccattg aaaatagtga
      181 cttttacgaa aaatttgcta aaatttacat tccatatagt taatataaaa cacttcctat
      241 taaatccaca ttaggaaatt ctccgagatt tacattccat atagttaata taaaacccca
      301 aaataaactt agtattttca acacttacac gtatacaact ctcctaaatt tgcagtgaac
      361 gagcaatagt gcaattgata acgcttatca tgcactctca acaccttata tttcaactgt
      421 tacgccaaat tttatcacaa atatagctca ctgcaaaatt tctataattt tattatatca
      481 taaatatatt acttttgaat attgatacca atttatacca taataaaagc aagaaaaaac
      541 gtaatctatc tacattggat ttaaattcta taacttaaag ttttttacat aatatttatg
      601 cttgtttaat caatctaata tatcattctt gtatattgct tttaatagtc ttttttctta
      661 aataaatatc tatctgaaca agcatagttt aattaaaaac ttttgtattc ccttaaaact
      721 gtatctacct cttattattt tatttatgat atattatttt taatctaaaa atagatatct
      781 atttttatta tagttatccc tttttaaaat actttaatca tatactactc ctaatttaac
      841 atcccttgaa atgctatttt cttatattgc aatttataat tgatatatct tcaatataat
      901 aatttaatgt atttttgatt ttaaagcttt tcagtgatat tattgataaa ttagaattaa
      961 ttatctagct ttaaaaggtc aaagatttct gttgctgtct gcggttttcc aaataaatat
     1021 ccttgtatgt aatctggctt tatttgttta actaaatcat actcttcttg tgtttctaca
     1081 ccttctaaac atacttttat tcctacatca tgacaaattg caacaataaa atgtataaat
     1141 gtagcatcaa acttactctt taatatatct ttaacaaata ctctatcaat tttcactata
     1201 tctattggag aaaattttaa aacttctaga gacgagtatc cagtaccaaa atcatccata
     1261 gcaatataaa ttcctaaatc ttgtaaagcc ttaaatttaa actgtaaaat actcatattt
     1321 tgtactgtat gactttctgt caattctaaa acaagatttt gataaggaaa attttcatca
     1381 tctataattt ttactatatc ttcaataaat gtgtcttcta atatttgaac agctgaaacg
     1441 tttacactca ctttaaaaaa aggatagtaa tcaatccatt tcctaaaagt acgtaatgcc
     1501 attcgaagca cccaagcccc tacaatattt atcatatcat tttcttctaa tattggaata
     1561 aattccaaag gagaaattgc tttgcatttg tcattagtcc accttaaaag aacttctaca
     1621 cctattattt tatgactttg tgtatctact tgtggctgga atcgaagact aaatccccta
     1681 aaatcatcat taattgatgc ctttaaatcc cgcatcattt ctaatgaata cattttattt
     1741 tttaatattt cttgggagaa aaaaacaagc ctgtttttac catgctcttt cgcatattgt
     1801 aaagagtaac tagcgcattt ataaagttct tttactgtat ctccatgttt tggatatatt
     1861 acacaaccag cagaaattgt tatatttaat ccatattttc tccatagttg aaggtgtata
     1921 atcatattct gtatttgatt atatagagtt agaatttcat tttcttcaac attatcaaca
     1981 agaataccca tttcgtcccc atcaagttta taaagttcag cattatctgg taaaatagat
     2041 tgtatagact gtgctagtgt ttttattata ttatctccaa aatccctatc atacatctca
     2101 ttaacatttt taaaatcgtc aatatttaat aatactatgc ataatttttc aatcatagga
     2161 tttgaaattt tatcttcaaa tacactcata aattcatgat aattcagtaa ctgtgtcaat
     2221 gaatcaatct tattttgttt acctaattgc gtcattattc ctgcaaaaat acttggttct
     2281 ccaaattcat cacgcattag ttgtcctcta catcttaacc atatatattc tccactacga
     2341 tttttagccc taaattccac tgtatgataa tccatctggt ttttacctat ttcagtgttt
     2401 gacttataaa aacgattcca gtcttctgga tgtacaattt ttttccagta aataagcgga
     2461 ttttcaacaa tttctcctgg taagtcaaat aattcaactt gtgatggtga atatcgaaat
     2521 acgcctgttt tcatattaca aatgtatata tattcatctg tacctctaat caaagcatca
     2581 taaagcaatg tagaattata atcaaaattg tctcttgaaa aattaaaaac tccttttcct
     2641 tctttagtat ttgatatttc attaaattca ttaatcttaa ttggtttatt taagattgtt
     2701 gagtttgcaa tatctattga ttgatgcata tgaacaactg tgccatcttg ccatgtaatt
     2761 aaactatcat aattttcaaa aacacgattt aacttattac atttttcata ccactttatt
     2821 aatactcctt ttttatcatt ctttaatagt tctaaaactt tacaaaaact acaagtagaa
     2881 ttttgctcag gatataatac ttgccagcaa acttttcctt ctggatctaa aatattatat
     2941 tcttctttca ttttcttgtt cataaaaata atttcattag tatgaatatt tgtaacatat
     3001 atattaatct gtagttgatt taatataact tcaaaattac gtttgttcat atacttatct
     3061 cctccatagt tactgctctt tataacaaca ctgttaaaat tattcttttt agaagattta
     3121 cttataactt atacatacac actaaatacc ttaatttaat tagaaagttt attatttcat
     3181 attttaaatt ataattgata aaaaaaaata cagcaatata gtattaccaa tttaaaaaaa
     3241 attaatatta cataatacat ctctctctta tattaaattt taacttggta ctaatcttga
     3301 caaattgtcc acaaaaataa attatatatt ttattattgt tttaaatttt tataaaaaat
     3361 cagtattatt gatactattg aagttaaaaa gcattaattt ctaaaaacat cataattttt
     3421 ctaatagact taatcacttt taatatatat agtaaaacaa aaaacttata ttttataaat
     3481 atcataattt ttttactgat attttattat ataaatttta tagaattaat tgtcaatttt
     3541 ttatgaaaat ggtaagatat ttatggatgg tatcttgtta taagaactat actttaaatg
     3601 tttttaatta ttttgtctta tgtaactcat atcctcagga atgagattag aaattaagat
     3661 gttgtatcac aaagtggagg tgatactatt aaaataggct ttattggttc tggaaaagtt
     3721 ggaacatctt tggcaaatta ttttttatca aaaggttatt gtgtttcagg cttttatgga
     3781 aggaatcaat tatctttatt agaatcaata aatttaacaa aaacaaagat ttacagcaat
     3841 ttgagagata taatttatga aaacgatata ttatttatta caacatcaga tgattctatt
     3901 gaaattatag acaaaaaact ttccaaattt aatttaaaac ataaatacat ttgccacaca
     3961 agtggctctt taaaatctag tatattgttc tgttcaaaaa aagcaggtgc attaatttat
     4021 tctatccatc ctatatttgc attttccaat aaaaatacca acataaaaaa aatgaaagac
     4081 atttgttttt ccatagaagg tgataatgaa caagatgatt tagttattca aaatttcata
     4141 gatggtttgg gaaaccaatt ttttataaga gataaggata cttcatccac ttatcatcta
     4201 gctaatgttt tagtttcaaa tttagtatta tctttactag atataggagt aagttacttt
     4261 attaaattag gtttatctga agaggaatct ttaaaagcta tcagtccttt agtaaaaaaa
     4321 aatatagaaa atatacttga taatggcttt actaaagctc ttactggtcc tgtttctcgt
     4381 ggagatataa caccaataag gaaacatcta tctgtattga ataaaaaaga tgaggatatc
     4441 tacaaaatac tatccttaaa cctactaaaa ataattgcct taggaaatga caattcatta
     4501 aaaggtacaa aaaatattac tatagaaaat gcaatcgaaa atttaataag taaatctgaa
     4561 aaatacaaag aaatttacaa acttttagga ggaaaaaacg atgaaaaata ccatacagac
     4621 ttttaagaac gctaaatatg aaggaaaaaa actatctatg cttactgctt atgactactc
     4681 aattgcaaaa ataatggatg aatgtgatat aaatggtatt ttaataggcg attcattagg
     4741 aatggtcata aaaggtgaag aaaacactct atctgttact atagacgaaa ttatatatca
     4801 tacaaaagct gtaaaaaatg gtgcaaaaaa tgccttaatc gtaagtgata tgccttttct
     4861 atcttaccat gtttctatag aagatgcagt taaaaacgct ggtagactta taaaagaagg
     4921 aggagctcat gctgtaaagt tagaaggtgg ctcaaacgtt ataaagcaaa tagaaagcat
     4981 tgtaaatgct cagatacctg taatggggca tttaggatta actcctcaat cagtaaattc
     5041 atttggtggg tttaaggttc aaggaaatac cagtgaaaca gcaagacaac ttattgaaga
     5101 tgctaaatta atagaaaaag caggtgcatt ctctattgtc cttgaaggag ttcctacgaa
     5161 aatagctgat atggtaacta attctatttc aattcctaca ataggtatag gggcaggaat
     5221 taactgcgat ggacaaattt tagtttatca agatatgttg ggagtgtttg gagattttgt
     5281 tcctaagttt gtaaagcagt atgcaaatat aggagatata atgaaagatt caattaaaaa
     5341 ttacatccta gaagttaata caggtgcatt tccacaagaa aaacacagct tttcaataaa
     5401 tgaatctgag ttagaaaaat tatactaata ttaaggatgg tacaaaatgc ttgtaaaaga
     5461 aattaaatta ctaagaaata taatcaaaga ttggagaaaa catggctatt ctatagggct
     5521 tgttacaaca atgggatttt tacatgaagg acaccaaagt cttataaaaa aagctgtaaa
     5581 agaaaatgat aaagtagtag tgagtgtatt tgtaaatcca actcaatttg gaccaaatga
     5641 agattttaat agctatccaa gagatataga taaagatttt aaatattgta tggatagtgg
     5701 agcaacagta gtctttaacc catcaccaga agaaatgtat ttaaaaggta attgtactac
     5761 cattaatgtt tctggactta cagattttct ttgtggagct aaaagacctg ttcattttgg
     5821 aggtgtctgc ctagttgttt caaagtttct aaatatagta acacctgata aagcttattt
     5881 tggagagaag gatgcccaac aattggctgt aattaaaaga atggtcaagg atttaaacat
     5941 aaatacagaa ataataggct gtcctataat tcgtgaaaat gatggattag caaaaagttc
     6001 gagaaataca tatctctctg aggaagaacg aaaatctgct ctaatattaa ataagagctt
     6061 aagtttagca aaagaagaat tagtaaaagg caatctaaat ccaaagaata taaaagaatt
     6121 aataacagct aaaataaatt ctgaacactt agcaaaaata gactatgttg aaattgtaga
     6181 tagtgagaca ttacaacctg ttaagcaaat agaacattct atccttgtgg caattgctgt
     6241 gtttatagga aaaaccagac taattgataa ttttacattt gagttaaata tatagtatat
     6301 aattttatat aatatatcta aaaaataata gaatacctaa aaagtaataa attacctaaa
     6361 aaataataaa ttgcttagaa aataatagat tacaataact aaaattttaa aattaaattc
     6421 cttaaaagta tgattaatta taattatata agtatattct tattacactt atattaattt
     6481 atattaaata tctactaact caaatattca taattaaact ttaaactgga taaattttct
     6541 tttaagaatg aaagtttatc cagttttgcg attataataa ccagttttac atatccactt
     6601 gtaaaatata tctaagcaat atatcatact atatagattt atgagtgaaa taatatctgc
     6661 tatttactga atatttttat ttttcgtttt ttagggggat tagttgtgtt taaagataag
     6721 attgatgaat gtgttcatat aatgactgca tatattgcta gtttaaaaga atactattca
     6781 ttcatagaaa ctcaaattgg tgattttatt aaaaaatatg gagaagatgt tgtagagtta
     6841 tgtctccata gagtgatgat tctactatgc gaatgtggtt tggcataaaa ttaaatttat
     6901 taaaatgggt aagaagaatc ctatataata ttcttcttac ccattttaat atatcaattt
     6961 catagcttat cgtttaaaat attttacctc cattactaga caactttaca ttctcttttt
     7021 aattctagta tatttaaatc attttagttg ctccttattt attcttatta cttgttttat
     7081 tttataaata aaataattct ttttaataat gttttttgtt gatattccta tcacacatcc
     7141 aattatttat cattgtcttg aaattgtttg catacttaat ttaatccaat tgaaacaccc
     7201 cagcatagaa tcccctgtaa aatagaagtt ggattaaatc ctaacatcca tacagaaaat
     7261 actactccaa gcacaagcaa tataatggaa tatatttatc acttaatttt ttatactttt
     7321 taaatcccat acctaaaaca ttaagtgcag ctattagaat taataattgt tgtggtataa
     7381 aattatgatt gcttcatatt tattcatttt ttgctataaa agttcttgtt tcacaaaaaa
     7441 tatttgtttt gtatcaaaat aatacttata aattacataa ttctcataaa aatttctagt
     7501 attacttgtg ctcttaataa ataatagcca acaagtacct taaatcatta tatctatatc
     7561 taataagaaa cacagtaaga taattttttc gcccataact taataaaaac tgttgatatt
     7621 agtagcaatt gaatttagtt aatttattta taatatacat aaatttaaat attacgaata
     7681 aagtattata aataggatac tttctaaagg gaggaaatta tgatagataa tcaaaaatat
     7741 gttattttat cactagaatt acatttattt ttttcaagaa ttatgaaaga gcatgctctt
     7801 tttttagaag caggattcac aaataaaaat tataatcttg ctatggaagc tgaccactat
     7861 aaaaagcaat ttgaagattt attatcatac actgttagtg ctagtaatgg tataattaga
     7921 cctgatatat tatattcaga agagcttgta actactctca catcagttgc agaacaaaaa
     7981 acagaagagt ttacagggat agaaataaac aaagacatca ctacaagaga attaaatcta
     8041 caaagtggtg taaacccaca agttggtcaa gacttagtga actatgtagc tcaacttaac
     8101 tctgatgcaa taagattact tgatgggctt attaatttta aagaaagagt cttagatggt
     8161 gtactatcat gtactatatt tacatcaaac taccctctac ttcttgaaca tataatacat
     8221 gaagcaaatt tatatcgttc ttatgtagtt gaccttgaaa ataaaataga tattgagtca
     8281 aaaaacgcta aagaaataga attattctgg gaccatatta tgatggaaca tgctctgttt
     8341 atgagaggat tactagaccc ctcagaaggt gaactaataa atacttcaaa tgattttgct
     8401 ataaaattta atgaattaat tgaaaaaaca aacgaaatga ctgattctaa tatcaagaac
     8461 attacagaag aaactctaaa tgaaactgtt gagtttaaag attttaaaga agcaggagca
     8521 tcaggaatag aacagtgtaa gataaaatct ataatattac cacttttagc agaccatgtt
     8581 ttaagagagg caaatcatta tattagaata ttggagagtt ataaaaacat gtaattaaaa
     8641 attactttta aa
//
LOCUS       DAELXX010000140         8628 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.142, whole genome shotgun sequence.
ACCESSION   DAELXX010000140 DAELXX010000000
VERSION     DAELXX010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8628
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.142"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            46..930
                     /locus_tag="KPH29_003270"
     CDS             46..930
                     /locus_tag="KPH29_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBG1468837.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPCIVHKEEFEHPLKLDISSV
                     VNGEVRQNSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
     gene            1029..2204
                     /locus_tag="KPH29_003271"
     CDS             1029..2204
                     /locus_tag="KPH29_003271"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HBG1468838.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEELLKDIPTANKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            2332..3186
                     /locus_tag="KPH29_003272"
     CDS             2332..3186
                     /locus_tag="KPH29_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468839.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSNESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKIKDDSSC
                     LYLDIYND"
     gene            3230..4465
                     /locus_tag="KPH29_003273"
     CDS             3230..4465
                     /locus_tag="KPH29_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBG1468840.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREIVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSESKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKSISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYISLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNEKTSVDMSKKTW
                     DGLAYRINEVILMDELCLV"
     gene            4566..5507
                     /locus_tag="KPH29_003274"
     CDS             4566..5507
                     /locus_tag="KPH29_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HBG1468841.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(5736..6089)
                     /locus_tag="KPH29_003275"
     CDS             complement(5736..6089)
                     /locus_tag="KPH29_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBG1468842.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMIW
                     LEKLKEDNTYEIYSI"
     gene            6385..7296
                     /locus_tag="KPH29_003276"
     CDS             6385..7296
                     /locus_tag="KPH29_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HBG1468843.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
     gene            7556..7990
                     /locus_tag="KPH29_003277"
     CDS             7556..7990
                     /locus_tag="KPH29_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468844.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            8013..>8628
                     /locus_tag="KPH29_003278"
     CDS             8013..>8628
                     /locus_tag="KPH29_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468845.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EISLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKD"
BASE COUNT         3430 a          810 c         1543 g         2845 t
ORIGIN      
        1 aaaaattaaa taatattaat caacttattg aatggaggaa taaaaatgaa atttgtgact
       61 ttttgtagta gcaatgaaga aaaaataggt gtatttaatt cagaaacaaa tagtatatat
      121 gaaattaata gtttaggatt aagtaaatta tatactgata tgaatgattt tattgaaaac
      181 gtatctacag gagatttaga aaaaataaaa aacaattcat ttgaaaacgc aaagtgttat
      241 aagttagaag aagttaaatt atgttcccca attgtaagac ctaaaaaaga tattatatgt
      301 ttaggtctaa actataaaga ccatgtcaat gaaatcccag atggagttat aaaaaacgta
      361 gttatgccag attatccaat ttatttttct aagagagcag acaaaataat tggagtggat
      421 gataagataa gcttacatgg agatttggta gagaagttgg actatgaatc agaacttgca
      481 gtaatcatag gtaaagaagg tataaatata tcaaaagaag atgcttatga atatatattt
      541 ggatatacaa tagtaaatga tataagtgaa agagcacttc aagataaaca tgttcaatgg
      601 tttagaggaa aaagcttaga cacacataca tctatgggac cttgtatagt acataaagaa
      661 gaatttgaac atcctttaaa attagatata agtagtgtag taaatggaga agtaagacaa
      721 aattctaata cagagtactt tatatttgat atacctacta taataaatga tttatcaaga
      781 ggaatgactc taaagccagg agatataatt tcaactggaa ctccagcagg ggttgctatg
      841 gggatgaatc ctcaagtata tttaaagcat ggcgatatag ttgaatgtaa agtcgaagga
      901 ataggagttt taaaaaatat agttgattaa tggataaata tgccatataa ttttattagt
      961 acaaataatt taattaatat aggattgatc aaacttatga ttaatttatt atagagaggg
     1021 atgagtatat ggcaagagaa ctagaattaa agtatggctg taatccaaat caaaaaccat
     1081 caaagattta tatgaagaat ggagaacttc ctatagaagt attaaatgga aaacctggat
     1141 atataaattt tttagatgca tttaatagtt ggcaacttgt gaaggaatta aaagaagcta
     1201 caggtcttcc agcagcaact tcatttaaac atgtaagtcc agcaggagca gcagtagggg
     1261 tacctttatc agatacttta aagcaaatat attttgttga tgatttagag ctttctccat
     1321 tagcttgtgc ttatgcaatg gcaagaggag cagatagaat gtcttcttat ggtgatttta
     1381 tagcactttc agatgtatgt gataaggaga ctgcaactat tatagcaaga gaggtttctg
     1441 atggtataat agctccagga tatacagatg aagcccttga aatattaaaa ggaaaacgta
     1501 aaggcaacta taatattgta aaaatagatg aaaattatac tcctgagcct attgaaacaa
     1561 aggatgttta tggaattact tttgaacaag gtagaaatga aatattaata aatgaagaac
     1621 tattaaaaga tattccaact gcgaataaaa tttttacaga tagtgcaaaa cgtgatttaa
     1681 ttatagctct tattacatta aaatatacac aatcaaattc tgtttgttat gctaaagatg
     1741 gacaagtaat aggggtaggt gcaggtcaac aatcacgtat tcattgtact agacttgcag
     1801 gtaacaaagc tgatacttgg tatcttcgtc aacatcctaa agtattaaac ttaaaattta
     1861 aaaaggatat aggtcgtcca gatagagata acactattga tgtatatctt tctgatgatt
     1921 atatggatgt gcttgctgat ggcatatggc aaaacttttt tgaagaaaaa ccagagcctc
     1981 taactggaga aggaaaaaga gcatggctta aaactttaac aggagtagca cttggttcag
     2041 atgcattttt cccatttgga gataatatag aacgtgcaaa aagaagtggg gtaagtttta
     2101 tagcacaacc aggtggttct attcgtgatg ataatgttat attaacttgt aataagtata
     2161 acatagtcat ggcatttact aaaaatagat tattccatca ttaaaatact gaaagtataa
     2221 agataatata gattaagtaa aaatctacaa caagttttgt tgtagatttt tttatttgct
     2281 gtataatttt aaatagaact ttatttttag atattcaatt tggaggttga tatggcttta
     2341 aatataagaa actatagcct attatgtaat caaggaacag ctaaatttaa tgaagatgta
     2401 gtcggtataa atccatttgg ggcatgggtt ttagatggtg ctacaggact taatggaaaa
     2461 aatttagtgt caaatgaaag tgacgcaagg tggtatgttc agtggtggaa taggtattta
     2521 tataaaaata taaataaaga agaatcatta aagagtatta ttaatactgg aataatagaa
     2581 gtaaaaaaag agtatgaact aagattaaat gggtttaagg ttgaaaaatt agatttacca
     2641 tctagttcta tagttgttgt aaaatttcat gaaaataaag tagagtattt tttattagga
     2701 gattgtgctt tgtttattaa aaatggagat tccaaagtta taaaagatag gagtatttgt
     2761 aaatttgata atactgtatt tgatgaaatg agtaaacttt ctaacttgga agagatggca
     2821 tttgatgaga ttagaactag tgttatggat acaatagtaa gcaatagatt gaagaaaaat
     2881 acgaaagagg gctattggat attggatttt gatgaggaag ctgtggaaaa ttctatatat
     2941 ggatacattg aaataaaaaa agattttcaa ttgatgctaa cgagtgatgg ttttacaagt
     3001 gcatgtgata gatataatct aataaaagaa gaagaattga tacgtatagc agaaaaattt
     3061 ggtataggat atatacataa taaagtaagg gattttgaag ataatgacta taaagcggtt
     3121 aaagtgccaa gatttaaaat aaaagatgat tcatcttgtt tatatttaga tatatataat
     3181 gattagactg tataaaacta tttaatttta gaaaggaggc aagtgaaaag tgaaaattct
     3241 tcatataata acacaaaaac caaatagtac aggtagtggt atatatttgt ctggtatgat
     3301 aaaagggttc gaaaaaatcg gtcataagca agctgtaatt gcaggtatag atgtaaatga
     3361 tgatgtaaat tgttttccaa gtgaagtaag tttttatcca gtaaagtata attgtgggga
     3421 gttaaatttt cctgttgtag gtatgagtga ttctatgcca tatgaaagta caagatataa
     3481 agatttaaat atagacatga taaatagatt gaaatatcaa tttaaagtaa atatagataa
     3541 ggctatgaat gattttaaac cagaacttat aatatgtcat catttgtacc tcttaactgc
     3601 atttgttagg gaaatagtaa aagatataaa ggttatgtct atttgtcatg gaacttgttt
     3661 aagacagtta aataccattg atttagagaa agaatacata atagcaaata taaggaaatt
     3721 agatttaata tttgctcttc atgaaaatca gaaatatgat attataaaga cttttggagt
     3781 aagtgaaagt aaagtagttg taattggtag tggttataat gatgatatat tttataataa
     3841 aaattataaa ataaaggatg ataaaataaa aatagtattt gcaggtaaga tatgtaaatc
     3901 aaagggattg attccattta taaaatctat atctaaatta aaatatagta aagatttaat
     3961 agaagtcaat tttgcaggta ctggaagcga tatcgaatca tataatgaga tagttaaact
     4021 ggcaagtaag tctccattta aaatgaattt cctaggaaag ttagaacaga gagatttagc
     4081 tgagctattc aacaggtcac aaatatttgt gttaccatca ttttatgagg gattaccagt
     4141 tgtagtttta gaagctttat cttgtggcac agatgtaatc actactgata ttcttggtgt
     4201 taaagagtgg attggtagtg agattaataa ttctggaaag atagaatata tttctctacc
     4261 ttttatggag aaagaaggta ttccaaaaga tgaagagtta tatgattttg aaaataattt
     4321 gtataatgca atagattcta agatacaatc tttattgaat aactcaaatg agaaaacatc
     4381 tgtagatatg tcaaaaaaaa cgtgggatgg cttagcatat agaataaatg aagttatttt
     4441 aatggatgaa ttgtgtttag tctaaaatta tttaaattat aaaaaagtgt aactattttt
     4501 aaactttttt taaatgattt tatattatac taatactaaa tatatttgtt ttagggaggt
     4561 aatttatgag gattagtaaa aaggtattgg cgttaggtat ttcagcatta ctgttaagtg
     4621 ttagttttcc aacatcaata aacgcactag ataagattga aaatattcaa ggagtagata
     4681 aatatgaaac agctgggctt atttcagata agcaagattt tacaacagca gttcttatca
     4741 atgcagattc tacaatggta gatgggattg ctgcaagtgg tcttgcagga gtaaataatg
     4801 cagctatttt attaactaat aaggatgata taccagaagc aacacttcaa agactgaata
     4861 gagttactaa gatatatgta ataggtggag aaaattctat aagtaaagat gtagaaaaaa
     4921 tgttacttat gagaagaatg caagttataa gaattgatgg tgtagataga gtagatacaa
     4981 gttataaaat tgcaggtgaa atagaaaaaa ttaaaaacag tgataagatg tttttagtaa
     5041 atggatttaa ggatgaagct gatgcagtaa gtgttgcatc tgttgcttat agagatggag
     5101 ctcctataat attgacaaaa gatatacctt cagcagaaga agacacagac ttagacccat
     5161 ggtttggagt ttctcctgtt ccagtatatg ctattggagg agagtctaca ttaagtgatt
     5221 acatagtgag cagatataga gcaactagaa ttggcggtgt tgatagatat caaactaata
     5281 aaaatgttat tgaaaagttc tataatggag caaaagagtt ctatataaca agtggagacg
     5341 atttagttta tgctttagtt gcatcaccac ttgcaaaaaa tgctcctgtt gttttagttt
     5401 caaataaaag tgataagagt atattaagtg gagctagtaa agtaacagca ataggtataa
     5461 gtgataagag tattatagag caatgtttgg atgctgtaaa gaagtaatgg agtgttatta
     5521 gtaaaatgaa tatatacatt gaaaattagt agattgttca ttttataatg gtataaaata
     5581 tatgggaaga ggctatcaaa tggagataag cctctttttt attgataaaa ttatgaaaat
     5641 atactttatt ttatataaaa aaagtatctc atgaactaaa tgtctatttt gatatactat
     5701 ctccttttct tatattatat gtattatatg attttttata tggaataaat ttcataagta
     5761 ttatcttcct ttaatttttc taaccaaatc atctctaaaa tatctagttc ttctaaatag
     5821 tctttctctg ttttgttatc atcatgtttt ataagttcta aaacttcaaa tgtaaatgct
     5881 ttttcaccat attttttcca ttcattattt aattctttta tcatacatga gccagcacca
     5941 agctgaaatt tgagagagtt aaatttgctt tttatgtcat tactttttcc aaggtaaatc
     6001 tttttagtaa tatcattttt tattatgaat acacctgtgt caaatttcat ttctttgtat
     6061 aattgtttta attcacgttt tctatccatg attttagata cctctcttta taattgatat
     6121 gtaatatgta agtttaaaaa tatattacat atcaattata aagagattaa ttttatatgt
     6181 aaatagtttt tataaattta attatgaatt agtcatcaat aaacaagaaa tttagctgta
     6241 gaaatccatt ataaaggcta ttataaattt taatatgcta aaaatataaa aagaatagtt
     6301 gacagtatag aaaatatgga ataaactaaa agtgtaatag taatacgaaa ttatatatta
     6361 cattgaaagg tgtgaatgat tattatgaat aaaaaaatag aattaatagg gaattttgca
     6421 agtgattgga ttaggtatag tgattatgaa tataaaatta ctgaagaaga tgaattatat
     6481 atagttccaa cagaagatgc tgtgtttacc atgtataacc catttgatgt agcagatgat
     6541 ataatagtag atataattcg cataggacaa gaggcattac aagatatatc aaaagagaca
     6601 aaagaaatga aactaaagaa agaagtactt gaatttgtaa aaaagtatgg attattgggt
     6661 ttaatatatt caagtgtata taatagagat atagttggag aagaaagagt actgatgatt
     6721 gaaaagaatt atattacaaa agagaatata ttgagtacag atgaatatgt aaacatgttt
     6781 ataccatttg caagtgatga agatgtgtat tttaaaaaat atagaagggg tatagatgta
     6841 tcaaagagag aagaatctcc aaagtttttt ggaaagagac cattgatttt agatttagta
     6901 ttttcaaagt tctacactga acaactagat tggattgtaa gttttgctaa agatatgtgt
     6961 aagcatttta atcaaatttg tgtatataaa aatacatcta atctattaac tgaaagtgtt
     7021 actattatgt ctggagggtt taatcctcaa aagataggat tttctataag ccaattagat
     7081 aaaccaatta ttgcatggga gtttgattct ttgaaaacag ctattgagac tatctatgcc
     7141 tttgcaatta caagtgaacc ttcttcttta aataaatgca agtactgtgg taaaatttat
     7201 attcctacaa attctaaatc tcaatactgt tcaccttctt gtagaaattg ctcaaatgta
     7261 aagaaaagta gaagtagagc caaaaaagat aaataaaatt aataaatata tcattgttta
     7321 gattttagta aataactact gaattaaaag ttgttataga aaattatata tctaaatgat
     7381 ggtatttttt tgttgagaaa attgaaaagt ttaattgaga tagtaatttt gagttgacaa
     7441 aaaccacttt taacactact attataagtg gttggtaaaa taatatacac taatttaaat
     7501 atagcataaa aaacaaaaaa agtcaatgaa aaaaataaaa aaggagaatt taaaaatgat
     7561 atcacctatt attttgagtt ctataaatca gaatttaaag gaaatagaaa gaaatgaact
     7621 gcttgaaact aatatagaat ctggagatta tggtctagct ctttctgaat ctgatgtaaa
     7681 agatataata aattcgagag ataatacttt aaaggggtat ggaagaatag aattagatat
     7741 aaaagttaca aaacagttaa tagaaaatat atatacatca caatatacaa atgtggacaa
     7801 ttatttagag gctattaatg atatgcaaga aattttttat tatttaaaaa atgaaactga
     7861 tgacaagata tgtgatgatg agattataga aatactgggt gaattttatg aaaaattttc
     7921 aggcaatatg gacaatgtaa gaggagaagc agacgaattt gcaaagaaat ttaaatttgg
     7981 agaagtttaa tatatgatag gagagatttt gtatgttaaa taatttaagt aatattaata
     8041 taaacgaaaa taatttaata aagaaccaat attccatatc tctacttaag gaatgtctaa
     8101 attgtaaagt gatagatgaa agagaagtat ataatataca acaagaaata tctctaatac
     8161 ttatggattt gattaaaaaa tatacaaatg gtcaaagcac atcagtaaaa acagaagttg
     8221 ccgaaaaact gttgatttca atatggtatg ctattgatgc atatataaat aagcttggag
     8281 atatagaaaa aagagttgtg gagttgactg ctaagaatgt taaaaaagtg taccttggag
     8341 gcattgaaat attagaggaa gagttagtta ggctgaaaga tttttataaa ctaatgatga
     8401 gtagaaaatt ggatactaat tcaatagcat ataatgatac cttgatggag ttatcaagtt
     8461 tctttaaaag ttatattgta aaatttgaag ctcaggacgt tccagtaagt atagattatc
     8521 cattggcact agatgatatg gatgttaagg gtatttatta tgtgaagaat tatattgaga
     8581 gtatagatat agaaaataga ttttgtaacc tatttaagaa aaaagata
//
LOCUS       DAELXX010000141         8561 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.143, whole genome shotgun sequence.
ACCESSION   DAELXX010000141 DAELXX010000000
VERSION     DAELXX010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8561
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.143"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            152..1372
                     /locus_tag="KPH29_003279"
     CDS             152..1372
                     /locus_tag="KPH29_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG1468846.1"
                     /translation="MQQALKLKYQTKELGKDFVIESCNSIKPWLINIRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKESADKTICIRADMDALPIEEEN
                     NIPYKSIHSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKF
                     LVEDGCLENPKADYIFGLHVMPHIETGLIETKYDTLNASVDTIKISIKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAAVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKIAKIVGDTASAFGCVGTLHVSDENYPAVINEKELVDTVISSTKELL
                     GEEKFILRPNPSLGGEDFSFYTEHCKGAFFHLGCKNEEKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
     gene            1409..2758
                     /locus_tag="KPH29_003280"
     CDS             1409..2758
                     /locus_tag="KPH29_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG1468847.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYSLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKNGGAYLYSKKAFGDFIGFEVGTMSWFIRIISWSTL
                     AVGFATALGSFWPESATEYKGCIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFVIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     IALITTIFICAIIYILIQIVCMGILGDRLFENSIPIADASSVFLGNYGKVFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPMVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISREKIQ"
     gene            complement(2890..3183)
                     /locus_tag="KPH29_003281"
     CDS             complement(2890..3183)
                     /locus_tag="KPH29_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468848.1"
                     /translation="MISMIMMLIGVLFIVFGIILTAVEIKGKMPGFYGVGQNAVRKNA
                     IGIIAAGIALIFFRNGLDSIDSILTYGVFLVIELVVIGLYFLIRIKYDKDGNL"
     gene            complement(3667..3870)
                     /locus_tag="KPH29_003282"
     CDS             complement(3667..3870)
                     /locus_tag="KPH29_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468849.1"
                     /translation="MSFGKRIEMNNIGVETRGVCTIMCPYTCKMMASSSCNCKNNDYN
                     ARGGSYNTYYNKLIHGQRTPDGM"
     gene            complement(3921..4172)
                     /locus_tag="KPH29_003283"
     CDS             complement(3921..4172)
                     /locus_tag="KPH29_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide maturation system acyl carrier-related
                     protein"
                     /protein_id="HBG1468850.1"
                     /translation="MNYTEKELLLENIFFDVTGINFNKNNNLKKELFFSPSLHIQPRE
                     LLLVFCEVIENFNIKIPEEKLLNRQFTSFDNVLKIINMV"
     gene            complement(4188..5258)
                     /locus_tag="KPH29_003284"
     CDS             complement(4188..5258)
                     /locus_tag="KPH29_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04066 family peptide maturation system
                     protein"
                     /protein_id="HBG1468851.1"
                     /translation="MEKVLIYPFNYNFFTILNHCNKISDIEINSLVSPSGWGLCNKEY
                     NVNNIITKVTNDFQSELKKNSLVWFVDSKENLDLEKFIIPKLKVALKEGKKILYTRDD
                     LNKISRYISHYEFKKINEWNLEKYEIRDIEGISQINTPIIFICGLYGGLDTFDVNIRI
                     GEELRKRGFSILQFGPKKESYLFKMIPIPEFMFDDSISSKTKIINFNHFIKDKEVEAK
                     PDLIIIEVPGELFPFSRKMVGNFGIRAFEISNAVINDCGILCIPSSIYDREYCEELKY
                     FIKGRHGINIDYFSIEKKVIDTYETEVKQKFSYMSLDENFFVEDIEDYEKTFFIHDNK
                     QLLELVDNIVNQLKEYAKVISI"
     gene            complement(5287..6708)
                     /gene="ccpM"
                     /locus_tag="KPH29_003285"
     CDS             complement(5287..6708)
                     /gene="ccpM"
                     /locus_tag="KPH29_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-rich peptide radical SAM maturase CcpM"
                     /protein_id="HBG1468852.1"
                     /translation="MNQKPFIHTFKAGKSYFIYDVNTDKILKVNAAVYNYLNKIEHNL
                     EKESDWNIEIEDEINTLINYGFLKNKRVNKTMHPETKYIKYHIENNLSSIILQVTQNC
                     NLRCDYCIYSGSYSNRVHTNKRMNIDVAKRSIDYLIKNSSQLNEVSVGFYGGEPLLEF
                     DLIKQIVEYVKVVGEGKEIYFNLTTNATLFNEEIIEFFVKNNITTMISLDGGKEIHDK
                     SRKFANTGEGSHDVVLKNVRYIKNKYPEYYKKNITFNTVINSENDYISIDDFISKNET
                     LKDSNFLASTLNNNYSDKEIVSSDEYILGKSYDRFLTLLNKLKFIKDESMSPLSRIER
                     ALLDDFRYEKNIEGRLELPDVWHHGGPCIPGAFRIFVTTDGEFFPCERVSESSKMGMI
                     GNLDSGVDIEKAEKLLNIGKLYEEQCSDCWAYSYCDICIAKIDDTKCNENEIKPHDCD
                     RIRHSTENNMKDYCVLKSLGYQY"
     gene            complement(6730..8502)
                     /locus_tag="KPH29_003286"
     CDS             complement(6730..8502)
                     /locus_tag="KPH29_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1468853.1"
                     /translation="MENGKFQFFKGVRNCFNMIYLHDKRYLFISMFMAIFQGIVPSIS
                     LLVSQNIINNMQTFSKSVKEILVLLIMYLIINIIPSIFQSFYGYYRIKFNKNFEKHIE
                     TMMLEKSLTLSLKDFEDDNTYDMINRAQSQKGFNITILSENLISIIRESVSTLSLIVV
                     LSQFQLWLIIVVIVIPVLKSLYSIKLGKEQYDVSVNRTWKERKAWYIDYLLSKGYAYK
                     EIMVFRIGSKLINHYKKLKEDIISQDMNIAKKNFIATLLCTVLDSIVSAGIIGFIIIQ
                     GILKKILIGDATTYIQAVQSIKDNVESIFSLFSSVIQDLFYTDLLFKFLDYNCKEDSI
                     EHIKIFEKIGDINKIELINISYKYNHSSEYSLKNINLTLNKNDPIAILGKNGSGKTTL
                     LKILLGFYDDYEGDILINGKNLKDIEKDEYIRKVSCMFQDYIKYEATLKENVAMGDVY
                     KSNLEEKVKSKLKETKLKPSIYEEDGLETILGTWFGKKQLSMGEWQKVAISRTLMKDA
                     DMYIFDEPDSSLDSEAGNELLSLYKKTFENKIGIFITHHIQHAKKITNNIVVLENGKL
                     LESGRHDELMDKKGVYFNLFCNCK"
BASE COUNT         3089 a         1274 c          941 g         3257 t
ORIGIN      
        1 actatgcaat cattttttat actatacata tagataaatg attatattta ttcaatattc
       61 ttataaaaaa caaaattctc catcgaaaat tatggcataa taattgctta ttatattaat
      121 aaaacaataa aaagcaattg gaggaataaa aatgcaacag gctttgaaat taaaatatca
      181 aaccaaagaa ttaggaaaag attttgtaat agaaagttgt aattctataa aaccatggct
      241 tataaacata agaagagaat tacataaaat tccagaatta gctcttgaag aaaatcttac
      301 taaacagaag gtaatttctt atttaaaaga aatcggaatt gattatatgg aatttacaaa
      361 acacaatgga attatggcat atattttaaa agaaagtgca gataaaacga tttgtataag
      421 agcagatatg gatgctcttc ctattgaaga agaaaacaac ataccttata aatcaataca
      481 ttctggaaaa atgcatgctt gtggtcacga tgcacataca accatgctac ttggagcatg
      541 taaagtttta cattcaataa aagataaact taatgtaaat gtaaaattct tatttcaacc
      601 agcagaagaa ggttttggag gagcaaaatt tttagttgag gatgggtgtt tagaaaaccc
      661 caaggccgat tatatattcg gacttcatgt tatgccacat atagaaacag gccttatcga
      721 aacaaaatat gatacattaa atgctagtgt agacactata aaaataagta tcaaaggtaa
      781 aagagctcat ggtgcatatc ctgaaaatgg tattgatgca atagttacag cttcacaaat
      841 tgttacatca ttacaaacta taataagtag aaacttagaa ccaaataatg ctgccgtttt
      901 gactataggt aaaatatatg gtggtgatgc acacaatgta atatgtgaag atgtaaaact
      961 agaaggcact ttaagaacct taaatagcaa aacaagaaac tttatgattg ataaaatagc
     1021 taaaattgtt ggagacactg catctgcatt tggttgtgtt gggactttac atgtaagtga
     1081 tgaaaactat cctgctgtta taaatgaaaa agaattagta gatacagtta tatcaagtac
     1141 taaagaatta cttggagagg aaaaatttat attgagacca aacccgtctt taggtggtga
     1201 agacttttct ttttacacag aacattgtaa aggagcattc ttccatttag gatgtaaaaa
     1261 tgaggaaaag ggcttaattt cacctcttca tacctctagc ttcaatatag atgaagattg
     1321 tttaccaata ggtgttatga tgcatgtaat gaatactttg tattttaatt agtacagtaa
     1381 ttaataaaag aataggagat aaattaatat ggaaaaagct aagaaattag gtctatttag
     1441 tatgatatta ttagggatta atagcataat aggttcaggt atattcttac taccaggtaa
     1501 agtttatagt ctagcaggtc aaaacagtat gtttatatac atttttgcta cactcttagt
     1561 tctatcaata cttttatgtt ttgcagaagt tggaagcatg tttgataaga atggaggagc
     1621 atatctatac tccaaaaaag cttttggaga ttttatagga tttgaagttg gtacaatgtc
     1681 ttggtttatt agaattatat catggtctac tctagcagta ggatttgcaa cagctttagg
     1741 ttcattttgg ccagaatctg ccactgaata caaaggttgt atagctgcaa tactcgtaac
     1801 actactttca ataaatagct tatttggaat taaaagcaca aagataatga ataatgtaat
     1861 aactatagct aaattagtac cattaatagt ttttgtgata gttggaatat tctttataaa
     1921 atttgtaaat attgttcctt caggaaatgt agttaactct agtatgggtc cagccattat
     1981 attagtattt tacgcattta ccggctttga atcatttatt gtggcaagtg gagaaatgga
     2041 aaatcctaaa aaaaatcttc caatcgcatt aataaccaca atatttatat gtgcaataat
     2101 ttatatatta attcaaattg tctgtatggg tatacttgga gatagactat ttgaaaacag
     2161 tattccaatt gcagatgctt caagtgtatt tttaggcaat tatggtaaag ttttcatatc
     2221 tgtagcaaca ttaatatcta tatttggtat aaatataggt tcatcaatag taactcctaa
     2281 atgtggttct tctctagcag aagaaggttc tcttcctgca tttataggaa aaacaaataa
     2341 atatggtgct ccatatgtag caataataat atctttaatt tgttgtatac cacttgtgct
     2401 aactggaagt tttgaacaac ttgcagtaat gagtgtaatt gcaagatttg ctcagtacat
     2461 accaacttgt ttatcagtaa tagttttaag aaaaagaaca gatgttaagg cctcttttaa
     2521 gattccattt ggtcctgtta taccaatggt agctatttta ggtagcttat ggttgttaca
     2581 acaagcttgg gcagaagata tttcaaaacc tataactcaa aatagagtct taataggatt
     2641 aggggcaatg ttgttaatag ctcctctata tatatttatg aaaaaaaata aggaaatcga
     2701 gcataaaaat aaatcagaag attctaaatt atatatttcg agagaaaaaa ttcaataata
     2761 ttttatacta ttacttctca taaaatataa taaatataaa aaaattgtag ataaaactat
     2821 tttataaaat ttatctacaa tttttttata taaacatata tcaaaatcaa aatattatta
     2881 atttatactt taaagattcc catctttatc atattttatt cttataagaa aatataatcc
     2941 tattactact aactctatca ctaaaaatac tccataggta agaattgaat caatactatc
     3001 taacccattt ctaaaaaaaa ttaatgctat acctgctgct ataatcccta tagcattttt
     3061 tcttactgca ttttgcccaa ctccataaaa acctggcatt tttcctttta tttcgacagc
     3121 agttaaaatt atcccaaaca ctataaataa tactccaata agcatcataa tcatactaat
     3181 catctaatta cctccttcac ttaaggaaat ttcaatttct aaaatctaaa ataaataata
     3241 tttatttttt ataataatat tgtatatcca attaactatt ttggaaatta gtaccaataa
     3301 ataatgttta aatattgaaa taatatatta attctaatca atattattaa tatatataca
     3361 tttttacaaa tatatttaaa aactttactt ttaattctta ctatttaaat aagtataatt
     3421 tattataata acttttaatt aatttatact tttaaattgt aaaaatcacc cctaatcact
     3481 atatttacca ctataataat tgaagaggtg atttatataa cagatactat ttatgcagtt
     3541 attataaaat cacactctta attataatca actgcatatt tttataataa aaggaaataa
     3601 agtaagaaag ataaatttaa tcaaatatgc taaatcaatt taatagtata cttattactg
     3661 aaatatttac attccatcag gagttctttg accatgtatc aatttattat aatatgtatt
     3721 atatgatcct cctcttgcat tataatcatt attcttgcag ttacaagatg aagatgccat
     3781 catcttacaa gtataaggac acataatagt acaaacacct cttgtctcaa cacctatatt
     3841 attcatttct attctttttc caaaactcat tcatatcctc cccttttcta taagatcact
     3901 tactttatac ctttattaac ctataccata ttgattattt ttagaacatt atcaaaactc
     3961 gtaaattgcc tattcaaaag tttttcctct ggaattttta tattaaagtt ttctattacc
     4021 tcgcaaaata ctaaaagtaa ttctcttggc tgtatatgta aagacggtga aaaaaataat
     4081 tcttttttta aattgttatt tttattaaaa ttaattccag tcacatcaaa aaatatattc
     4141 tctagtagta attccttttc agtgtaattc ataattaacc tccaattcta aatagatatt
     4201 actttagcat actcttttaa ttgattcact atattgtcca ctaattctag taattgttta
     4261 ttatcatgta taaaaaatgt tttttcataa tcttctatat cttcaacaaa aaagttttca
     4321 tctaatgaca tatatgaaaa tttttgcttc acctctgtct catatgtatc tataactttt
     4381 ttctctatac taaaataatc tatatttata ccatgtcttc ctttaataaa atatttaagt
     4441 tcttcacaat attctctatc atatatactt gatggaatac ataatatacc acaatcattt
     4501 attactgcat tagaaatttc aaaagctcta attccaaaat taccaaccat ttttctagag
     4561 aaagggaata attctcctgg aacttctata attattaaat ctggctttgc ctctacctct
     4621 ttatctttta taaaatggtt aaaattaata atctttgtct tactacttat cgaatcatca
     4681 aacataaact ctggtatagg aatcatttta aataaatagc tctctttttt aggaccaaat
     4741 tgtaatattg aaaaccccct ttttcttagt tcttctccta tccttatatt tacatcaaaa
     4801 gtatctaacc ctccataaag accacaaata aatattattg gtgtgtttat ctgtgatata
     4861 ccctctatat ctcttatttc atatttttct aaattccatt cattaatctt tttgaattca
     4921 taatgagata tatacctaga tattttatta agatcatcac gtgtatataa tatcttttta
     4981 ccttctttta gagctacttt taatttagga attataaatt tttctaagtc tagattttct
     5041 tttgaatcaa caaaccatac caaagaattc tttttaagtt cactttgaaa atcatttgta
     5101 actttagtaa taatattatt aacattatac tccttattgc ataaccccca gccagaagga
     5161 ctaaccagtg aattaatttc tatatctgaa attttattgc aatgatttaa aatagtaaaa
     5221 aaattataat taaatggata tataagtact ttttccataa tcccatcttt cctttcatta
     5281 gaaaatttaa tattgatatc ctagagattt caaaacgcag taatctttca tattattttc
     5341 tgtagaatgt ctaattctgt cacaatcatg tggttttatt tcattttcat tacatttagt
     5401 atcatcaatc ttagctatac atatatcaca ataactatag gcccaacaat cagaacattg
     5461 ctcttcatat aattttccta tatttaaaag tttttcagcc ttttctatat ctactccact
     5521 atctaaattt ccaatcatac ccattttaga actttcactt actctctcac atggaaaaaa
     5581 ttctccatct gtagttacaa atattctaaa agcccctggt atacatggtc caccatgatg
     5641 ccacacatct ggtaactcta atctaccttc aatatttttc tcatatctaa aatcatctaa
     5701 taaggctctt tctattcttg ataaaggtga catactctca tcttttataa attttaattt
     5761 gttgagtaaa gtcaagaatc tatcataact cttacctaat atatattcat ctgatgaaac
     5821 tatttcttta tcagaatagt tattattaag agtggaagct aaaaagtttg aatcttttaa
     5881 agtctcattt ttagatataa aatcatctat agaaatgtaa tcattttctg aatttatcac
     5941 tgtattaaat gtaatattct ttttataata ttctggatat ttatttttta tgtaacgtac
     6001 attttttaat acaacatcat gagatccttc tcctgtattt gcaaattttc tacttttatc
     6061 atgaatttct tttcctccat ctaaactaat catagttgtt atattatttt ttacaaagaa
     6121 ttctattatt tcctcattaa ataaagttgc atttgtagtt agattaaagt atatttcctt
     6181 tccttctcca actactttta catattctac tatctgctta atcaaatcaa actcaagtaa
     6241 gggttctcct ccataaaagc caacactaac ttcatttaat tgactagaat tcttaatcaa
     6301 atagtcaata cttctttttg ctacatctat attcattctt ttatttgtat gaactctgtt
     6361 gctgtatgaa ccagaataaa tgcaatagtc acaacgtaaa ttacaatttt gggttacttg
     6421 taaaataata gaggatagat tattttctat atgatatttt atatacttag tttcagggtg
     6481 cattgtttta tttacccttt tattttttag aaatccataa ttaatcaagg tattaatttc
     6541 gtcttctatt tctatgttcc agtcactttc tttttctaaa ttatgctcaa ttttatttag
     6601 ataattataa acagcagcat ttacctttaa aatcttatcc gtattaacat cataaataaa
     6661 atatgatttt ccagctttaa atgtgtgtat aaatggtttt tgattcatta attaacctcc
     6721 ctttaatatt tatttgcagt tacaaaataa attaaaataa acaccctttt tgtccataag
     6781 ttcatcatgt cttccacttt ctaacaactt tccattttca agaactacaa tattgttagt
     6841 tatctttttt gcatgttgga tatggtgcgt tataaatatt ccaatcttat tttcaaatgt
     6901 ttttttatat aaagaaagga gttcatttcc cgcttcagaa tctaaagatg aatctggttc
     6961 atcaaaaatg tacatatcag catctttcat aagagttcta gatattgcta ctttttgcca
     7021 ttcacccata gaaagttgct tcttaccaaa ccaggttcct aaaatagttt ctaatccatc
     7081 ttcctcataa atacttggtt tcaatttagt ttctttaagt ttacttttaa ctttttcttc
     7141 caagttactc ttatatacat ctcccatggc gacattttct tttaatgtag cctcatattt
     7201 aatataatct tgaaacatac aacttacttt tctaatgtat tcatcttttt caatatcctt
     7261 taaatttttt ccatttatca agatgtcacc ttcataatca tcataaaaac ctaataatat
     7321 ttttaaaagt gtcgttttac ctgaaccatt ttttcctagt atagcaatag gatcattttt
     7381 atttaaagtt aaattaatat tttttaaaga atactctgat gaatgattgt atttatatga
     7441 aatatttatt aactctattt tatttatatc ccctattttt tcaaatattt ttatatgttc
     7501 aattgagtct tctttacagt tataatctag aaatttaaac agtaaatctg tataaaaaag
     7561 atcttgtatt actgaactaa ataacgaaaa tattgattca acattatcct ttatagattg
     7621 aactgcttgt atatatgtag tcgcatctcc tattaaaatc ttctttaaaa taccttgaat
     7681 tattataaat cctataattc ctgcagatac aattgaatct aaaactgtac ataatagcgt
     7741 agcaataaaa ttctttttag ctatattcat atcttgagat ataatatcct cttttaattt
     7801 tttataatga ttaataagtt tagaaccaat tctaaatacc ataatctctt tgtaagcata
     7861 accttttgac aacaaataat ctatgtacca ggcctttctt tctttccaag ttctgtttac
     7921 actaacatca tattgttcct ttcctaattt aatggaatat aatgatttca atacaggaat
     7981 aacaataaca actattatta gccaaagttg aaattgtgat aatacaacaa taagagaaag
     8041 tgtagatact gattctctta ttattgaaat aaggttttca gatagtatag taatattaaa
     8101 tcctttttga gattgagctc tatttatcat gtcataagta ttatcatcct caaaatcttt
     8161 taaactcaaa gttaaagatt tctctaacat cattgtttca atatgttttt caaaattttt
     8221 attaaacttt attctataat atccataaaa actttgaaaa attgaaggaa ttatatttat
     8281 gattaaatac ataattaaca aaactaatat ttcttttaca cttttcgaaa atgtttgcat
     8341 attattaatg atattttgac ttactaacaa agaaatagat ggaacaatcc cctgaaaaat
     8401 tgccataaac atagagataa ataaatatct tttatcatgt agataaatca tattaaaaca
     8461 atttcttaca cctttaaaaa attgaaattt accattttcc acaatacacc tccatactta
     8521 ataaaattga aataaaatat tttattaatt aatataatat a
//
LOCUS       DAELXX010000142         8535 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.144, whole genome shotgun sequence.
ACCESSION   DAELXX010000142 DAELXX010000000
VERSION     DAELXX010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8535
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.144"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            129..1634
                     /locus_tag="KPH29_003287"
     CDS             129..1634
                     /locus_tag="KPH29_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBG1468854.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
     gene            2024..3121
                     /gene="dinB"
                     /locus_tag="KPH29_003288"
     CDS             2024..3121
                     /gene="dinB"
                     /locus_tag="KPH29_003288"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBG1468855.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGKPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKSSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVEVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
     gene            3377..4513
                     /locus_tag="KPH29_003289"
     CDS             3377..4513
                     /locus_tag="KPH29_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1468856.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            4506..6053
                     /locus_tag="KPH29_003290"
     CDS             4506..6053
                     /locus_tag="KPH29_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG1468857.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVVVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMKHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKINEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            6387..7253
                     /locus_tag="KPH29_003291"
     CDS             6387..7253
                     /locus_tag="KPH29_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG1468858.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            7394..>8535
                     /locus_tag="KPH29_003292"
     CDS             7394..>8535
                     /locus_tag="KPH29_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBG1468859.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
BASE COUNT         3285 a          913 c         1544 g         2793 t
ORIGIN      
        1 ttaatcttaa ttgtataata ttactttgag tgaaaaagta ttaacatttt tttataagat
       61 gttttactaa aaaaatatat ataaatatac tgttatcaac aagaaattta atttagtgag
      121 gtgttaagat gaaagtagga tttgaccaca aaaaatattt agaagagcag tctaaataca
      181 tcttagaaag agtaaataat tttgacaaac tttatttaga gtttggagga aaattgatgg
      241 gagaccttca tgcaaaaaga gtacttccag gatttgatga aaatgcaaag ataaaagtat
      301 tacaacatat gaaagaaaaa gttgaagttg taatttgtgt ctatgcagga gatatagaaa
      361 gaaacaaaat aagaggagac tttggtatca cttatgatat ggaagtttta agattaatag
      421 atgatttaag aggttatgaa ctagatgtaa atagtgtagt aattactaga tttgaaggac
      481 aacctgctac aactgtattt ataaataaat tagaacgtag aggaattaaa gtttataagc
      541 attatccaac aaagggatat ccgtcagatg ttgagactat agttagtgat gagggatatg
      601 gagcaaatga atatatagaa actacaaagc caatagtagt agtcacagca ccaggaccta
      661 atagtggaaa acttggtact tgtctaagtc agttgtatca tgaaaataaa agaggtaatg
      721 tagttggata ttctaagttt gagacattcc cagtttggaa tgtacctgta aaacatcctt
      781 tgaatatagc ttatgaagca gctacagttg atttaaaaga tgtgaatatg atagattcat
      841 tccaccttga aacgtatggt gaaatgtcag taaactacaa tagagatttg gaattatttc
      901 cagtgcttaa aaaaataata gaaaagataa ctggaaaaga atcaattttc caatctccaa
      961 ctgatatggg tgttaataga gttggctttg gaataataga tgatgaagta ataagaaaag
     1021 cttctataga agaaataata agaagatact ttaaaactgc ctgtgagtat aaaaagggac
     1081 aagttgataa aggcgcttat gatagaatga aaatgattat ggaagagctt aacttaaagc
     1141 cagaagatag aaatgttgta ttacctgcta gaaattacag taataaatta aaagaaagtg
     1201 ccgacaagaa tgatacttgt cctgtagttg cattagaatt agaagatggt acaatattaa
     1261 ctggtaaggg ttctgattta atgaatggaa cagcagcagt agtattaaat gccattaaac
     1321 atttagccaa tatatctgat gatatgcact taatatcacc tgtaatatta gaacctatca
     1381 taaacttaaa aacaaagact ttagctagta gaaatgtttc acttagttgc caagaggtgt
     1441 taactgcact tagtatttgt gctgtaacta atccaactgc acagtttgct atggaaaagt
     1501 tagcattatt aaaagggtgt caagctcatt ctactactat attaaataga gatgatgaac
     1561 aaacatttag aaagcttggt atagatgtaa cttgtgaccc agaatatcct tcacaaaatc
     1621 tttactatag ttaaaattat ataaaaaaat atgggctgtt ttaaagtaat agtttgagca
     1681 gtccattaca attataataa aatcagtagg ctgtcttgta taagacagtc ttttgttttt
     1741 aaaacatccc ataaattaaa tatatatact gttatccata aggaatttta aatttagttc
     1801 cttggaatac ctattgtaaa gcttttatat agtttttgca agtgttttta aggatttttt
     1861 ataaattata ttgaagttaa tggattatag atggttttat ttatggaaaa tatgaatata
     1921 taaagaaaat aatgaaaaaa taccaaaaag aaacataagt ttggtataat taaaaagtaa
     1981 tataaataaa attaatctat ttatacttta aaaggtgata ataatgaaac attatagaaa
     2041 aataattcat atagatatgg acgcttttta tgcatctata gaacaaagag ataataaaaa
     2101 attaaaagga aaacctgtta ttgtcggtgg aaatccacaa agccgaggtg tggttgcaac
     2161 gtgttcttat gaagctagaa aatttggtat ccattcagct atgccatcag ctgtagctta
     2221 caataggtgt ccatatgcag tttttgtaag acctagattt gatgtttata agagtgtgtc
     2281 agagcaaata agagatatat tttatagata cacggattta gttgaaccat tatctttaga
     2341 tgaagcttac ttagatgtaa caaaaaataa aaaaaatatt gattcatcaa ttgagatagc
     2401 aaagcaaata aaaaaagata tatttagaga agttggtctt acttcttcag ctggtgtgtc
     2461 atacaataag tttcttgcta agattgcatc agacttaaga aagccaaatg ggttaactgt
     2521 aataaccgaa gaaaatgccc aggacttttt agataaatta cctgtgaata aattttttgg
     2581 tgttggaaaa gttacttcaa atactcttaa aaatttaggt ataaaaactg gatatgattt
     2641 aaggtgttta aatttatttg aacttgaaaa tatattcaaa aaaagaggtt atgaattata
     2701 taaatttgct agaggaatag acgatagacc agtggaacca aatagggtta gaaaatctgt
     2761 tggggctgag actacattaa gtcataattt ggatattgat gaagaagaaa cacgcaacat
     2821 tcttgatgaa ctttgtgaag aagtatgtca tagattaaaa agctctgaga agtttggaaa
     2881 aacattaaca ttaaaaatta aatatgagga ctttacaaag ataactagaa gccttagttt
     2941 agaacactat attgacgaat ataatgatat aagaagtggt gtagataatt tgttgagaaa
     3001 tgttgaggtt aatggaaaac aaattaggct attgggagtt acaatatcaa acttaagtga
     3061 taaaaaagaa acttataaag atattacatt atttgaatac atggatagta ttcaaatgtg
     3121 agttttgttt aaatgaaata acactagctt taatactata taattgaggc tagtgttatt
     3181 ttatgaatat ttttatactt tttgtttttt gacattatat actatatata aatactttat
     3241 atttgtagca atattcagac aaatagtgac ctaaaaatcg tactttgttt acataaataa
     3301 aatagtatga tataattata aaaacaactt ataatattgg agaaggttgc ttgttcgaaa
     3361 aaggggatga taaattatga tagagataag aaatgtaaca aaaaaaatag gaaataatgt
     3421 tatattagat gatatttctc tagttgtaga aacaggtaca ttggtagttt tgattggttc
     3481 aagtggttgt ggaaagacta ctactttgaa gcttataaac aaattaataa aacctacatc
     3541 tggtgagata tatattaatg gcaaaccaat ttctcaggaa aatgaaatag agcttagaag
     3601 aaaaatagga tatgttatac agaacacagg cctattccct catcttacaa taaaagaaaa
     3661 tatagagcta attccacgcc taaaaaagga aaaatccgtt gaagaaattg aaaaaagaac
     3721 gttgcaacta cttgagatgg taggacttga tagtgatgaa tttttgaata agtatccttc
     3781 agaacttagt ggtggacagc aacaaagaat tggtgttgca agagccattg ctactgatgc
     3841 tgaaataata ttgatggatg agccttttag tgctttagac ccaataacga gaacatcttt
     3901 acaagaacaa ttattttctc ttcaagatga attgaaaaaa actataatat ttgtaacgca
     3961 tgatatggat gaggctttaa agatagctga taaaatttgt attatgaagg atggaagaat
     4021 agcacaatat gatactcccg aaaatatact tagaaagcct gcaaatgact ttgtaaaaga
     4081 ttttatcgga gaagatagag tttgggataa tccagaatat ataaaagcaa gagatattat
     4141 gataaaaaat cctattgctg ttaattccac tagaactgta actcaaggaa tagaaattat
     4201 gagaacgagt aaggttgata gcttactaat tatagataga gcaaaaacat taaaaggtat
     4261 tgttactgta aaagatatga aagatattga tgataagtca atattacttg ctgatattat
     4321 gtcgagtgag cctttgcatg taaatgaagg tgataattta gtagaaatac taaatgtaat
     4381 gaatcgtaat tcggttggat atattccggt tatttctgat gaaaataaac tggtagggct
     4441 tattactaga agtagtttac tttctgtttt aagtgaacaa ttcttagaaa tggaggtgag
     4501 tgttcttggc taattttttt aactttatac tattacaaaa agataagatt atagagcttt
     4561 taatacaaca tatgagtcta actgttacat ctattttaat agctataata gttggtgttc
     4621 ctcttggaat aattatttca agaatctcat ctcttagaaa gtttgtactt ggatttgtaa
     4681 acttagtcca agcagttccg tctatggcac ttttgggtct tcttgtacct atacttggca
     4741 taggaagtaa accagccata tttatggtag tggtttattc attactacca attgtaaaaa
     4801 atacgtatat aggtataaca agtattgacc cagtagtatt agaatcagct aaaggtattg
     4861 gtcttacaag aaatcagaca ttatttaaaa tacaattccc tttagcactt cctataataa
     4921 tgggtggagt tagaatatct gcagttacag ctgttggttt gatgactctt gcagcattta
     4981 taggtgctgg tggtttagga tatttagtat tttcaggggt tcaaactgta aataataata
     5041 tgattttagc aggggcaatc ccatcatgta taattgcact gttagttgat tatttattta
     5101 gtaaaattga agttgcagtt actccaaaag gattaaatcc aaaagcacct aagaaaaatt
     5161 atatagcact taaagtaata tctgtagtgg ttgttgtttc aatgttgttc gttgttttta
     5221 gttctagttt ttcaagtaaa aaagatacaa taactattgg ttctaaagat tatacagaac
     5281 aacttatact tggtaatgtt tatgctgaat tagtagaaaa aaatacgaat cttaaagtta
     5341 agaagaattt aaatctaggt ggttcatctg ttgcgtttaa tgcaattaaa tctggtgagt
     5401 tagacatgta tgttgactat acaggtacac ttttggtaaa tgtaatgaaa catgctccga
     5461 ttaaagatgc tgatgaagca tataatgttg taaaagatac tatggaaaaa gaaaatcagt
     5521 taactttact tgaccctctt ggatttaata atacttacac attagctatg atgcctgaaa
     5581 ctgctgaaaa gtatggaata aatactattt ctgatttgac taaatatggt aaagaattta
     5641 cattctcacc aactttggag tttgaaaata gagaagatgg tcttgtaggg ctttctagag
     5701 attatggttt aaaattcaaa gatgtaaaag caatgaatgg aagtttaaga tatacagctt
     5761 tagataataa tgaatctcag gttatagatg catttttaac tgatggtctt attaaaaagt
     5821 ttaatcttaa gatattagag gacgataaaa atttctttgt accatattat gcagcaccat
     5881 tagtaagaga agatactttg aagaaatatc ctgagttaga aaaagttatg aatatgcttt
     5941 caggaaaaat aaatgaagaa actatgagag aacttaacta tcaagtggat gaacttggta
     6001 aatcaccaga agaagttgct cattcattct tagttaaaga aggcttagta taaaaaatta
     6061 atagtttata tcaaaaattt aagggagtgt ctcaaaatga actttttagt tcatgaggca
     6121 ctctttttta tatgaaatca aatatatttt atcatttcaa caataaaaaa gaagcttatc
     6181 tttattttga gacagtctct tatttttatg tataccaaaa ccatacctac gatttttatt
     6241 tataattaag tttcaaatag aatgaacaag tacaaccaat tttgaatata aatatagtaa
     6301 atgtgcttat atacgatttt attatctggg tgattgatat tgatagatag ctttctttat
     6361 tgaatcgaag ggagggggaa tttgttatgg ataactataa gaaagtgaag caagtattgt
     6421 ggataatttt atttgctaat tttgcagtag cattattaaa aatcattatt ggtaatcaaa
     6481 taaaaagcta tagtatgaca gcagacggat ttcattcttt gtcagatgga gcatctaata
     6541 tagtggggct tataggtatt ttctttgcat ctaaaccaaa agataaaaat catccttatg
     6601 gacataaaaa gtttgaaatt ataaccagtt tatttataag tggtatgttg tttgttatag
     6661 caattaagat aattcttagt gcagtgctaa gaatagctaa tccagttgta cctgctataa
     6721 ctatagaaag tttaattgct ttaattatta cactttttat aaatattttt gtatgcatgt
     6781 atgaatatag aattgggact aaactaaata gttatgtttt aatttcggat tcattgcata
     6841 caagaagtga tatatttgtg tctctaggtg tattggttac tttagttggt gttaagttag
     6901 gatttccagt aataatagaa tctatagttc ctattattat ttctgcattc ataatttatt
     6961 cggcttatgg aatatttagg ccatctattg gaattttggt cgatagagtg gctgtagatg
     7021 aggattatat aaaagaaatt gtgtttgaat ttaatgaggt aagggatgtt cacaatatca
     7081 gaagtagagg tagcaaaagt tctatttata ttgacatgca tgtaatggta gacccattta
     7141 taagcgtaga gcaatctcat gatttaacac ataaaattga gaaacaaata caagaagaaa
     7201 ttaatgaaaa tgctcaagta atagtccata ttgaaccttt ttatagtttt taacttagct
     7261 ataaaataca agttataaaa aaatataatt tataaaaaaa atattatacc ataaataatt
     7321 tataaaaatt atttatggta taatattttc tataaagaaa ttaagtctta atatatctaa
     7381 ggaggaattt acaatgagat acgttgaata tggaaagaca gggaaaatgg tttcagtagt
     7441 tggctatgga ggattaaggt ttgatttaga aaaaagtaat caagaaaatg ctgatttagt
     7501 caaatatgcg tatgaaaaag gtattaatta ctttgataca gcgccagggt attgtgatga
     7561 cagaagtgaa gatatatttg gactagcctt tagacaaatg ataaaggaag gtaagaccga
     7621 tttttatgta tcaactaaag aaaagccaaa agcatgtgat acatcagaaa aagctataga
     7681 ggctgtaaaa aaatcaatag aacgattagg cgtatctaaa ataaattttt atcatgtttg
     7741 gaatattaga aagatggaac attacgaact agcaatgaga cctggaggtc aatatgaagg
     7801 attattgaaa tgtaaagaag aaggattgat agaccatata gtattttcat ctcatcaacc
     7861 aggagatgag gttatagata ttctaagtga aaataagttt gaaggtgtaa ctatgggaat
     7921 aaatatatta aactttccat ataggctaaa aggtgccaaa tatgctgttg ataatggata
     7981 tggtgttgtg gcaatgaatc cattaagtgg tggaactatt ccaaagtata ataaagaact
     8041 ttcttttcta tcaaaagatg gcgaaagtac tatagaatca gctttaagat tcaacatagg
     8101 aatttcacaa ataagtatat ctttgatagg gtttaataaa aaacaagata tagatgatgc
     8161 atgtaaaatt gcagacgaaa atagaattta ttcagatgag gatatacatg atattgagac
     8221 tagattaaat aagaatatga acgaaatatg tacaggttgt ggttattgta aagtatgtcc
     8281 aaaaggtatt aataccccag cctatatgct tttttataat gaaaagcaga tgtttaaaaa
     8341 gagtgatgaa gaaatgacaa agttagttta tggtttagga cattggaatt acacaatgaa
     8401 tagtaaggca aaagctaaag aatgtatatc ttgtggaaaa tgtgaagttg aatgtactca
     8461 gcatttacct ataatagata gattaaaaga aattaaaaaa tgggaagaag atggagctaa
     8521 tagtgttaaa gtcta
//
LOCUS       DAELXX010000143         8476 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.145, whole genome shotgun sequence.
ACCESSION   DAELXX010000143 DAELXX010000000
VERSION     DAELXX010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8476
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.145"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            230..1309
                     /locus_tag="KPH29_003293"
     CDS             230..1309
                     /locus_tag="KPH29_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBG1468860.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKSTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDEINWDL
                     SISALERNTEYWSSEFAVRAFIIKDEERMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIAKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDTTSINLQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYMKSNGKRNRKIFKISEVSLKENEKKSYMKKHSFADVSVRRH
                     YPGIHSITIIINGVEKGKLDFELGV"
     gene            1633..2001
                     /locus_tag="KPH29_003294"
     CDS             1633..2001
                     /locus_tag="KPH29_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBG1468861.1"
                     /translation="MDVYSFWNDTLAQDAVKIRKYFHEDAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVERVLEFGNLVITVTHVYAINKPLSFHVVSFISLKDDKIISIDEYW
                     GEDDDVPQWRLDKHIGKPIK"
     gene            2118..2921
                     /locus_tag="KPH29_003295"
     CDS             2118..2921
                     /locus_tag="KPH29_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG1468862.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDKQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKLLRNIISK
                     MCGATSEGRRSMMKMLEGYEKKELCHLMYLGFAGFMPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPSELNQIMDIFIKQLNI"
     gene            3306..4196
                     /locus_tag="KPH29_003296"
     CDS             3306..4196
                     /locus_tag="KPH29_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG1468863.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSARFDAVKELNDSGIFCGILLTPMLPFLTDNKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVLKYQKTYGLNYVCTIQNKDSCEKLLREECARYGILTD
                     MKEIIKAYKRSESYIQIKFF"
     gene            4659..5102
                     /locus_tag="KPH29_003297"
     CDS             4659..5102
                     /locus_tag="KPH29_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG1468864.1"
                     /translation="MIEIKTIEINKKSQIDLLMLADPCEEMIDKYLNKGTMYALYDNK
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
     gene            5274..5444
                     /locus_tag="KPH29_003298"
     CDS             5274..5444
                     /locus_tag="KPH29_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468865.1"
                     /translation="MDSNNNTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            5995..6903
                     /locus_tag="KPH29_003299"
     CDS             5995..6903
                     /locus_tag="KPH29_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBG1468866.1"
                     /translation="MTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFINDI
                     LKDAPYANRNIKPFTHIKRIIKAETDPTINYIGEKRPRLYILAEDEESNHINIEMQRA
                     LEDDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLTM
                     DGHPNIYSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIKR
                     ALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGVEKGKREEKFNIIIKLKNK
                     GYNLSEICDIIDDLNKSEVEKIYNQN"
     gene            7083..7571
                     /locus_tag="KPH29_003300"
     CDS             7083..7571
                     /locus_tag="KPH29_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HBG1468867.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     ITVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
     gene            7657..8064
                     /locus_tag="KPH29_003301"
     CDS             7657..8064
                     /locus_tag="KPH29_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468868.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            8065..8283
                     /locus_tag="KPH29_003302"
     CDS             8065..8283
                     /locus_tag="KPH29_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1468869.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
BASE COUNT         3313 a          848 c         1499 g         2816 t
ORIGIN      
        1 agtaaagtat aaaattctat tactataata aaagatagaa ctagaagtct ttatgaaagt
       61 gggaaaagta tttatataaa taaatttata tggtttatag tttaacagta ggaaatatcg
      121 tatgagtgag aatatcgtat tttacataaa gatttagcaa tatgacacac tgttgtcgtg
      181 ttctgtagga tatgattaat aaatcagata tgaaaggaga gtgagtaata tgccagaatt
      241 actaaaaaat atgtataatc gtgagtcact ttatgaagtt gcagtagcta tccaatcagt
      301 ttacaactca tttaaggtag acgagttcat aaaatctaca atggatgaga catggaataa
      361 cttagaatta aaagccagat gtcgaaagat aagtatgagt ttaggaatgt atttaccaga
      421 agactataag gaagctcttt ccattcttga aaaatcagtt acaggttttt attttgcttt
      481 tttctttcca gattttgttg aagtttatgg gcaggacgaa ataaattggg atttatctat
      541 aagtgcatta gaaagaaata ctgagtattg gtcttcagaa tttgctgtaa gagcatttat
      601 catcaaggat gaagagcgta tgatggcaca gatgcgtaaa tggtctaagc ataagagcga
      661 acatgtacga agacttgcaa gtgaaggttg tcgtccccag ttgccttggg gacaagcaat
      721 atccaaattt aaaaaagacc ctacacctgt actgcctata ttggagcaac ttaaaacaga
      781 tacatcaacg tatgtacaaa agagtgttgc taataatcta aatgatatct caaaaacaca
      841 tccagattta gtcataagca ttgcaaagga ttggtacggt aaaaataaat ctactaactg
      901 gattgtaaag catggttgta gaactctact aaaaaaaggc aatcgtgatg tattagccct
      961 ttttggatat gatgatacta cttctataaa tcttcaggac tttactttag aaacaacgtc
     1021 tatttctatt ggagaggatt taactttttc atttaatatt ttagcaaaaa aagcgacaaa
     1081 aacaaggctc gagtacggaa ttgattatat gaaatctaat ggaaaaagga ataggaagat
     1141 atttaaaata tcggaggttt cattaaaaga aaatgagaaa aagtcatata tgaaaaaaca
     1201 ttcatttgca gatgtaagtg taaggagaca ttatccaggt atccactcga ttactattat
     1261 tattaatggg gtagaaaaag gcaaacttga ttttgaatta ggtgtataat tttactaaca
     1321 gcataaccta tatcagatac tttgctaaac ctatatcgta tactctaata aatctatatt
     1381 aggtagttta ctacttgttt actgaattta aatacaaaga aatacaggtg ttaatccctg
     1441 cccattaaag gaatatgagc ataacacaaa agtagaactt atcaattctg ttacgggtat
     1501 agtagtaaat ccatagaatc acaattagaa gatgaagtgt aaaaaataag ttagtgttga
     1561 ttttagttgc tagtgatatt gaatagttat acaatagcca tataaattag aatttgtaga
     1621 ggagaaatgt aaatggatgt atatagtttt tggaatgaca ctttggcaca agatgctgtt
     1681 aagataagaa aatattttca tgaagatgct tatattaatt ggcatgatac aaatgagcat
     1741 tttactttag aagaatttat tattgcaaat tgtgagtatc ctgataaatg gaatggtaaa
     1801 gtagaaaggg tgctagaatt tggaaattta gtaattacag tgacacatgt ttatgcaata
     1861 aataaaccac tatcatttca tgtcgtttca tttatcagcc ttaaagatga taagattatt
     1921 tctattgatg aatattgggg agaggatgat gatgtgccac agtggagatt agataaacat
     1981 attggaaagc caataaaata gtgtttgttc tgttttataa ctgaaatttt gataacattt
     2041 gatattttga tttgtcattg gtcaacatag tcgttagtgt aataatttaa aaaagtagaa
     2101 tttggagagt gagcactatg gaagaaaaat acatcttatc tgaccgaggt aaagtatatt
     2161 attggataaa agaaagtaat agtgtgtttg caaagaactt aatttttttg ccaggactaa
     2221 cagcaaatca tcatcttttt gatttccaga tacagtactt ttcaaaaaat cataatgtat
     2281 tggtatggga cgctcctgct catggaaaat caagacctta cttagatttc tcatattcta
     2341 acttggtaga agaattgaaa gtaattttag ataaacaaaa tatgaaaaag gttgtactta
     2401 tagggcaatc ggcaggggga tttgtggcac agtcctttat tgtaaaatat ccaaatatgg
     2461 tagaaggatt aatgatgatt ggaacttgtc catatggaac tgactattat agtaagtcag
     2521 atattttctg gttaaaacag actgaatgga tgttaaagtt attcccagat aaattgttgc
     2581 gaaacataat atctaagatg tgtggagcta cgtctgaagg cagaaggagt atgatgaaaa
     2641 tgttagaagg ctacgaaaaa aaagaactgt gtcatctcat gtatctggga tttgctgggt
     2701 tcatgccaga aattcaagac ttaaatatac attgtccagt ttgtttggtg gttggagata
     2761 aagacaagac gggtaaagtg agaaaatata atgagcagtg gcacaataag actgggtatc
     2821 cattgcacat tataaaaaat gcttcacata atgtaaatgt cgataaacct agtgaactaa
     2881 atcagattat ggatatattt attaagcaat taaatatata aaaactacat actcattagg
     2941 taaattatgt agtatatgtg tttacatatg gttatagaaa taaatatgta ataaactact
     3001 taacacttaa aaagtggatt tgtccagagc ataatacaaa gcatgatata taaattttta
     3061 agcgaaatac atcaaaagaa gtattagaat tagtggagat aaaacagcta ttagagatta
     3121 taaataaata gttttaaagg tactacgaat atagaggaag gtaaatcata cgttattcaa
     3181 ggattataaa tgagtagttt aaaagtacta tcggataggt ctctgcatat ttaaacctta
     3241 gtaaaggtag ttaaagtagt agtttaaaaa ctaaaaaatt atataaagga attaggtata
     3301 agaaaatgat tgagagtata ccagcaaaac aaattttaca aaaagtgaaa tttgacaata
     3361 caagatggtt tggaattgac tataatatga atctctatcg tggttgttct catggatgta
     3421 tatattgtga tagccgtagc acaatataca acatagagaa ttttgacaaa gttagatata
     3481 aagaaaatgt gatagagatt ttaagtaaag aattgcgttc aaaaagaaaa aaaggggtag
     3541 taggcattgg agctatgtct gacacatata atccttttga aaagcaactt tgtattacaa
     3601 agcaagcact agatttgatt tcggaaaatc attttggagt gtctattgat actaaaagta
     3661 gtttggtggt tagagatata ccgattttgc agaaaattaa aaaaaataat tctgctattg
     3721 taaagttgac tataaccact gcaaatgatg aattatcaaa gaaaatagaa ccttatgtaa
     3781 atccaagttc tgcacgattt gacgcagtga aagaacttaa tgattctgga attttttgtg
     3841 gcatattatt gactcctatg cttccatttt tgactgataa taaagatgaa atacgagcaa
     3901 ttgtagaaaa agcacacaag gctaatgcaa agtttattta ttgcatgtat ggtgtaacaa
     3961 tgcgtagtgg tcagagagag tttttttatg agcatttaag agatatatca ccaaaattag
     4021 tacttaaata tcagaaaaca tatggtttaa actatgtatg tactatacaa aataaagatt
     4081 cttgtgagaa gttgttaaga gaagaatgtg cacgttatgg tattttgaca gatatgaaag
     4141 agattataaa agcgtacaag aggtcggagt catatattca aataaaattt ttttaagata
     4201 ggctttggat gagatataat agtggtgatt gatgtgttga tattatagtt gattgttaga
     4261 gaggatatta tagatgaata ttaatattga tattctaggt aattgctaga actaatatta
     4321 tagatgaata ttagtattga tattctaggt aattgctaga gttagtatta tagatgaata
     4381 ttagtattga tattctaggt aactgctaga gctaatatta tagatgaata ttagtattga
     4441 tattctaggt aattgctaga gctgatatta tagatgaata ttagtattga tattctaggt
     4501 aattgctaga gctgatatta taatttatta ttaatattaa tattctaggg aattatcact
     4561 atattttaaa aagtaaaaat atatatcaaa atgaactaaa taagtactgt aagaagtttg
     4621 ttaaattaag gtttgaataa agtgagggtg gagaacttat gatagaaata aaaactatag
     4681 aaataaataa aaaaagtcaa attgaccttt taatgttagc cgacccatgt gaagaaatga
     4741 ttgataaata tctcaataaa ggaactatgt acgcattgta tgataataaa gaacttactt
     4801 gtatagcagt tgtaaatgaa atttccaaag aaatatgtga attaaaaaac attgctacat
     4861 atgagcattt tcaaaatatg ggttatgcct caaagatgat ttatcatctt ttagatgttt
     4921 atagtaaaaa atattcatca atggttgttg gtacatctga aagcggagtt cctttttatg
     4981 agaagtttgg atttgtttat tcacataaaa ttaaaaactt ttttgtagat aattatccag
     5041 aaccaatctt tgaaggagaa ttacaatgtg ttgacatgtt gtatctatca tataactttt
     5101 agataggata atatttttag gtagatataa ttaattaatt catataatta tcggaattat
     5161 aatttataaa ttatgtataa taagagtata atttatttag aagctgttat attgtattat
     5221 attcaaataa gatatataat aatttgttta ttttgaattt tggaggatta attatggatt
     5281 caaataataa tactataaaa tcaactgtta aaaagggtat ttcttttggt tcttgtttag
     5341 caatgattat ttcttatact gcatggaaat ctattccatg ggctattttt catggcttaa
     5401 tgagttggat atatgtactt tattattggg ttaagtatgc atagattaaa ttaataaata
     5461 agtatgtaat attgtaaagt ttaagaaaca aaatgtaagg gagtgtctcg aagtgaactt
     5521 tttagtttat aaggcactct tttttgtatt aaataaaata tattttcatt acttaaacaa
     5581 tgaaaaagat gagtatctct attttaatac atacccttat attcattcat ttatcaacaa
     5641 tagttttaaa aagaaagcta tctaaaatct attgtaatct aagtgcttct ctttaaagtc
     5701 ttaactttag taatgatata taagtttata aggttcttat ttcaataatg caaattttac
     5761 ttagaagttc agactttggt atgaagaaat gggtaataat aaaaaagagg tgttgatatg
     5821 aaagaaaaat tagaagaaaa atctaagatt attaaaacat caaaagagta taaacgaact
     5881 ataattgaag aaaaggacag agaatgaacc aaaaaattaa atgacatact tagtagaggt
     5941 gatttttcta atgaaaaatt agataaaatc aaggatttaa ttccaaaaga aatattgact
     6001 agtgaaaatg ttggagaagt tgtaactgaa gatgggtatg ctaagcctga gtatgatgag
     6061 gtatttaaat cattgtttac tttaaataat gattacgatt tacttgcaaa ttttataaat
     6121 gatatcctca aagatgctcc atatgccaat aggaatatca aaccgttcac tcatataaaa
     6181 agaataatta aggctgaaac cgacccaaca attaattaca ttggtgagaa aagacctaga
     6241 ctttatattt tggctgaaga tgaagaaagt aatcatataa atattgagat gcaaagagct
     6301 cttgaagatg attatttgga aagagctgaa tattatctta gtcgtgtaca tggtagaaag
     6361 ttagaagaag gtaaagagta taaagaaatt ggaaaaactg ttggaataca tatactaaat
     6421 catgtaaaat ataatcacat agaagattat gtaaattgcc tgagattaac aatggacggg
     6481 catccaaata tttattctag taaaactgca ttatatttta tagaacttcc aaaaattcgt
     6541 aaatccagtt gtatagcaaa cagagttcta atatggggta aacttattga taatccttct
     6601 catattgata ttagaatact atctaaaaca gattatgtta ttaaaagagc acttgataga
     6661 ttaaaagaat taggcagtaa tgaggactat ttattaaatt taaagagagg tgcgtatatt
     6721 atgaataaaa gtagaaattt taaagaagaa atttttcaag aaggtgttga aaaagggaaa
     6781 agagaagaga aatttaatat catcataaaa ttaaaaaata aaggatataa tttgagtgaa
     6841 atatgtgata ttatagatga tttgaataaa tcagaggttg aaaaaatcta taatcaaaac
     6901 taatactata attgagatgg cactagataa atataaagct tcctctttta aatttaaatt
     6961 aatataaaac cttgttattt aactatagta gatacaaaga gatatataag ctaaggagat
     7021 gattcaaaat aaaagtttga actatctcct tcttatagaa aaacaaaggg gttgatttat
     7081 atatgaaatt ttatactata cagactttag aattttggaa caacaataaa aacaataaat
     7141 atctatcaaa tgattattct tttatattag aagactttga tattccatac aaatggatgt
     7201 atagtcaaat gacaaataga atatatgaat ttgatgatag tatgatatgg gtttggacga
     7261 agagacctga tttaagatgt agtggttatt tagaaaaagg aaaaaaaggt gttttacttg
     7321 aaatagaaat aagtgaagac aatgtgttat tatcagaatt tgatttatgg catttacctt
     7381 taatggatat tactgtagag ctttatgaag atgaacaaat tgaaaagaag gtaagttggg
     7441 aaagaatatt tgacttagaa ctgtgttcaa aattaaatga agttaatata gaagatttga
     7501 cattgcaagg agtgacatct aaagtgcctc tatctaatgt aaaattaata aaagaatttg
     7561 ttgcaaaata atatttttat tgaaatttat tcaaaaaagt tatatattta taacaagagg
     7621 tgttataaat acataacttt ttaaggggga ttttatatgt ctaaacaaca aactggtatt
     7681 aaatcaacac ttatttcaga aatattggga atttcttatt gtattggagc tacattagta
     7741 aataagtatt tttttaaagg aagtagtatt gaatatcttc ctataattat ttgggtatta
     7801 gctatgggag ctcagtgggt ctataataaa aagtatgatg atttaattga tgaggcatca
     7861 aaatgtatac tatcaagagt taatgatatt gcaataaaag tattgttttt ttctgtagct
     7921 atagtatcta tatttcttgt aactttggac ataaatgtgt caaatttaaa tataggaatg
     7981 tctttattag tcattttgtt tattcaatcc ttgttgaagt taattctatt tatatatttt
     8041 gacagaaagg ggatatacaa ttaggtgtca gtattactaa agacaaaaat taaatcctat
     8101 agagaaaaga atcttatgac acaaggtgaa cttgcaaagt cagtaggagt aagacgtgaa
     8161 actattgtac atttagaaaa tggtcgctac aatccttctt taaaactggc aatggatatt
     8221 gctaaggtat ttgatacaac tgtagaagag ttgtttgaat ttattgaaga aaataatatt
     8281 taaatttcat gtattactta tgcattagag aatgaattaa tagataaaaa attcaagttt
     8341 atgtattaga gaatgaatta attataggta aaaaaccaag cttatagatt agagaataaa
     8401 tgacttatag atgaaaaacc aagctcatag attaaagaat gaatgattta tagatgaaaa
     8461 agaaagctta tagatt
//
LOCUS       DAELXX010000144         8453 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.146, whole genome shotgun sequence.
ACCESSION   DAELXX010000144 DAELXX010000000
VERSION     DAELXX010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8453
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.146"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(18..248)
                     /gene="dltC"
                     /locus_tag="KPH29_003303"
     CDS             complement(18..248)
                     /gene="dltC"
                     /locus_tag="KPH29_003303"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBG1468870.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            complement(267..1415)
                     /gene="dltB"
                     /locus_tag="KPH29_003304"
     CDS             complement(267..1415)
                     /gene="dltB"
                     /locus_tag="KPH29_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBG1468871.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKHISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            complement(1415..2929)
                     /locus_tag="KPH29_003305"
     CDS             complement(1415..2929)
                     /locus_tag="KPH29_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HBG1468872.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVIFN
                     FSDERNFGDINVIDMDKLNDIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYKELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            complement(3050..4240)
                     /gene="dltD"
                     /locus_tag="KPH29_003306"
     CDS             complement(3050..4240)
                     /gene="dltD"
                     /locus_tag="KPH29_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBG1468873.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTFPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGINPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
     gene            complement(4528..5547)
                     /locus_tag="KPH29_003307"
     CDS             complement(4528..5547)
                     /locus_tag="KPH29_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBG1468874.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLHGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQAITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            complement(5723..6262)
                     /locus_tag="KPH29_003308"
     CDS             complement(5723..6262)
                     /locus_tag="KPH29_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HBG1468875.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPILVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            complement(6363..7043)
                     /locus_tag="KPH29_003309"
     CDS             complement(6363..7043)
                     /locus_tag="KPH29_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBG1468876.1"
                     /translation="MQKVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTDIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PQNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            complement(7072..8361)
                     /locus_tag="KPH29_003310"
     CDS             complement(7072..8361)
                     /locus_tag="KPH29_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBG1468877.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
BASE COUNT         2784 a         1508 c          822 g         3339 t
ORIGIN      
        1 aaagtctaac tactaattta ttttcttgtc tccaagaatt ttactaaatt atttacagta
       61 gacatatctt ttctctctaa atcagttggt tgaagctcta tccctaatct attttcaatt
      121 tcaagtaaaa cttcaattat agctaatgag tctaaaagtt ccgtctcaaa taaattaagg
      181 tctaaatctt ctcttatctc atcagtccct aatacatctt caaatatttc tataactgtt
      241 tcttgcataa attttcctcc ttaaaattaa gctaaataac ctgagaatat cagcaatcca
      301 aaacacacaa tatggaatgt aatgaaaatc tgcactctct cgaaccattt atctttttta
      361 tgttttttat aaaatttaga ttttcgctga tatatatcag ttaacacaag tgccaatcct
      421 tggtatacac cataagcaac ataataccaa gttagaccat gccaaaatcc cattgttatc
      481 atggttatca tctgagccac atgagcagca gtagtccttt tcttaaatct tttctttctc
      541 attgatgaca taacaaatct agaaaaaata taatctccaa accatcttga aagacttata
      601 tgccatcttg tccagaactc tttcatatct ttactgataa atggcttgtc aaagtttata
      661 ggtacttgta ttccaaatat gtaccctgta cctatagcaa ataaactata tcctgcaaaa
      721 tcaaagaaca ggtataaact atatgcgtac atataactta aaatatttat aaaactcata
      781 tctttaggta ttcttgatac ccaatatgta tttatcaaaa atgctataac aaacttataa
      841 cctactccca tgacaatatt ttttattcct ggcagtaagt attcttctat atactctttt
      901 ctagagatgt gtttttccaa gtcctgttca aatcttcttg acctatcaat aggtccagaa
      961 cttaaagttg gaaaaaataa cataaaataa agcatctttg aaattttaac ttcttttata
     1021 gctccatcat aaatttcaat aaccatttgt atagttctga aatttaagta tgatattcca
     1081 ataaatccaa ttattccaaa agaagtaact ggagatattt tatttattat tattgggagc
     1141 attgatgcaa acaagaatcc ccaatatatg tatttatttt tagtttttct tcttacatat
     1201 tcatatcctt taactattat tacttcaaga ataataaata ttaccaaata ctttagctgg
     1261 acatcaattc ctactattaa gaatatcata aacaaacttg ccaacatacc ataatacttt
     1321 atattcttgc ccattagacc taaaattact gctggtattg atgttaataa taatatataa
     1381 agataaaaat aatctccata ctgtgaaaaa atcattaaat ttcctccata agtttctttc
     1441 tgtctatttt accattgata tttgttggaa actcagagat tattgaaata tttcttggaa
     1501 tcatatatga aggtatatac tttccaagtt ccttttttat tatcatccca tttttaatat
     1561 tacttaaatc attcttttca tttaactcaa caataccttt taagtaagca attttttcgt
     1621 ccttatatac tggtaaaact actgcatttt ttacattatg tacttttctc aaattatttt
     1681 ctatatcttc tatttcaatt ctaaatccat ttaatttaat ctgaaaatct tttcttccac
     1741 aataatatat atttccatct aaaagatatc ccatatctcc agttttataa gctctccact
     1801 ttacaccatc tatttcatca tagaaaaata cttcatcagt cttttcttta ttattgaagt
     1861 aacctttaga aactgatggt ccaattatta taatttcacc cttttcattt tctttaagtt
     1921 catttccatc ttcatcaagt atttttattt tacaatttga catagggtat ccaactggaa
     1981 gacttttttc atcatctata gctttttgag tcatatcatt tacacttaca ccaacagtag
     2041 cctcggttgg tccatatcca ttgataattc ttgtgtttgg aaatctactc ataagttctt
     2101 tagtaagatt ttttgaaagt gcttctccta tgaatatcat tgactcaagg ttaggaagca
     2161 ttttactatt aaactctttt tcagttatac atactcctgc aaaagaagga gttgacaccc
     2221 atacagctat atctgagata ctaaattgtc taaatagttc tttatagtct gctaatacat
     2281 cttttgatat tgaaaataat gtagctccat ggataagacc agggtatatt gttgtaactg
     2341 aaaggtcaaa agaatatgct ggttggttca ttattacttt ttcacttcca tctatgttta
     2401 gatatgggct tatccaatct gaaaagctat ctaaattgtt tgaacttatt tgaacccctt
     2461 ttggctttcc tgtacttcct gaagtaaata gtatatatgc attttcatca tcttttaccc
     2521 agttttcttt atctaatgat tttccctgat attcatttat tatatcattt aatttatcca
     2581 tatcaattac attgatatct ccaaaatttc tttcatcact aaaattaaat attacttttg
     2641 gttttatctc tttagtgact tcaaaaactc tatcaattgg aaaactaata tcaagaggca
     2701 catacgctct acctgatttt aaagctccta tcatacatgc cattatcatg ttttctttat
     2761 ttccataaat tactatagga gtatcctctt ctttgtatac atcctttaga aatactgata
     2821 tagcatctga atattcattt aaatctttat aagaaagttt atctccatta cacattagtg
     2881 ctgttctatc tgtatttgaa tattttttta ttccttcaat aattttcata taagaatgct
     2941 cctttaaaat ccgttataaa taaatggcaa ttctttaaat ggagtaaatg ttagaactcc
     3001 tatcgccaat attataacca tagttaaaca aatactagtt aacaaatctt tattgttctt
     3061 taaatatttt aaataatctc tcacagacat ctacccatcc tttcgtacca agatgcatta
     3121 catcccttaa ataatactta tctgaccctt tatctttaag gtttagaact tcaaagcctt
     3181 tgctttctgc tattttttgt gctttatcgt agtactgatt tctttctttt tcagaaatac
     3241 cagtcaaatt atagaattta tccatacttg ggattaatac aacaactgga ttaataccta
     3301 agtcagtaca tacatttagc atcagtttat aattttcaaa ctccttagat gttaataaat
     3361 ttacatcttt gtctcttcct ttgtattgct ctataccatc tttaaaatgc tctttataat
     3421 agtacttatc aatatttagt ggatttttac caactctttt tttagcatct tcaatggctt
     3481 tctttctctc atatgaccaa tttattggtc tttttctttt tgttgctctc tttttatcta
     3541 gcttaattaa tcgtttataa agaataccct tttctttaag agctatacag tactttcttc
     3601 cttgaaaata aggctctaaa agcacctttt cagctttttc aggaaatgtc ttaggttcat
     3661 atagcttagc atataatgct tctgctttat actcatcact tccccataat agcttacttg
     3721 atttttttgc atattcaatc ttattttgtt tagatatttt tggattatct aaaaatcgat
     3781 aaaattgtat tggtgaaaat ctagactgat aatggtgact tgtgactccg tctttttcca
     3841 taaaccattg catagaaatt aacaagacaa ctttcttatt atcaatatta ggattcatac
     3901 ttcctaatat tgcagcatct tgtaatgtct gagtataggc tctcccaatt gcaaacacct
     3961 tatttttact cctatttgta ttaaaatagt actttggatg ttgttttgtt gaattactaa
     4021 gttctgatga ccccaaaatc ataatatcct tttctcttaa aaaatgatta tttgctgtaa
     4081 cccctttatc ttttatatca cttaaagtat catccataat tggaagtaga tttttctccc
     4141 tcaaaagttc atcagtttta gaatcaagaa atttgtctaa cccaaataaa aatactacgc
     4201 ctattataaa aggtgtaata aagtatatca attttctcat attttctctc ctctaaaaat
     4261 attcaaattt atgttattat taatcatatt agttttgata tgtatattca actacaaaat
     4321 tgtaataaaa tcgtcaaaac tttttttgat actgttacaa aattgtaaaa aaaaagaaaa
     4381 aaacaccaaa aataaatttc tttttagcac tttttcatac ttataatttg acattttaac
     4441 acttggctaa gtatacacct taccttaact taaaattaaa tttatttaag aatatatatt
     4501 ataaattctt atattttcta atataagcta tatactatca gctggataaa caatactact
     4561 ttgtcttctt atctcatcca taatttccat tactattatt gaattttcat gagaatttat
     4621 atttgattct attttattgt tatttataag acttataaac tctacaagtt cataatacat
     4681 tccttctatt tctctaggct tttcctctgc tttttctttt ggaatagata tatcttcttc
     4741 tcttccatct ctgtatttaa taatagcttt ttcaaataga ttaagctttt caattattat
     4801 acttccctct tctccttgta tttcagatgg tatataagaa tcagctactt tagaatactg
     4861 aattattgca tccatatcat catattgaaa tacagctgaa ccttctccat ccactccagt
     4921 ttgaagtata tgagaaactg cctttacagt ttttggagca ccaaataaat tcaccatagg
     4981 atgtatacaa tacactccta tatccattaa agctccattt gacaactctt ttttaaaggc
     5041 attttctata attccatttt tatatttatc atatcttgaa gaatactgac aatagcttcc
     5101 aaaaaatctt cttattttcc ctattttata caagttttct ttaactgctt taaagtttgg
     5161 aacacatgta attctcatag cttccataag caatacatta ttatctttgg ctgtttttat
     5221 cattgatttg acttctttta aattagatgc taacgacttt tcacaaagca catgtttttt
     5281 gtgttttaaa caagtaatgg cttgctttga atgtaatgca tttggagaag ctatatatac
     5341 agcatctata tccttcgatt tagccatttg ttctaaatca tcaaaaaaaa tacttgctcc
     5401 atgtagagtt ccaaattctc tagctttttc taaatttcta gaatatacag caaccaattc
     5461 aaaatcttta accctagatg cagctcttag aaatatattt gctatattgc ttgtcccaac
     5521 aataccaaat cttattttac ccatcataaa tcactcccca ttttctacac tttattttaa
     5581 ttttaataaa aaactttaca acttatagat atattataaa ataattttat caaataaaaa
     5641 agaaagctaa ttttaaatga tttcaccatt ttaacatagc tttcttttaa ctacaatcta
     5701 aattcaactt atatttttaa agttatcttt ctactatatc tttatttccc ctcaaaaatt
     5761 ttatagtaag aatttcatcc acaaacaatg ttactaaaag tatagttaca agaattggcc
     5821 aataaggtat tataagacta tataaactat caactatata cacttgaaaa tatctaactc
     5881 caataaatgt caatatagtc caatatacag aaaactttag acatactatt cctctactat
     5941 tgtacttata atctgaataa tcccaataga ccttattaaa atatactttc ataagtattc
     6001 ctgtaatata ttctactact gtcggcacta ctagacataa taaaagcaat gcaaatatat
     6061 tattggggtc aataaatctt gagacctcta ttataatcga cattgcaatt gcatacattg
     6121 gtttaaatgg accatttaaa aatccatctt cttgaaaatg cccttgttta taataactat
     6181 ataagttttc aattatccaa cctataaaac catacaagaa aaaattaaat aaaaaatata
     6241 atagagaacc tacattatcc atgaatattc ctcctagctg tttatactta ttttttctca
     6301 tattcatgga tttattctaa atcaaagaaa ttatatttaa tttatttaat taatacttta
     6361 tattatagat tgtgacattc tttagagttc aaacttttta ggtattctct aacttccagt
     6421 aaattttcaa gtatcttatg tgattgtgat ttaatttgct catctggctc ttttaaatct
     6481 ggtacattta tacatcttat cccaccattg tatgcagctt caactcccat aggcgaatcc
     6541 tctatgacaa tacaattttg gggattaaca tttatctttt tagctgcttt taagaatatc
     6601 tcagggtttg gtttagagtt tacaacatca tctccacaaa ctatagcatc aaagtaatct
     6661 tttaaatttg ccttagctaa tctctttact gctctttctc tctttgttga tgttgcaaca
     6721 gccattttgt atccattttc ctttaaaaaa gagattagtt cattgactcc taatttgata
     6781 ggagctccct tcctctccat aaattcaatc atatttttag ttttttcgtc atataaatct
     6841 attattggta cagaactatc gtatatatct gtaagacctt ctattattcc tttacgattt
     6901 cttcccataa ctgatgtata aatttcctta gtcatagtat atccatactt ttcaaacgtc
     6961 tccatccaaa attcaagaga aattctctct gagtcaaaca aaactccatc catgtcaaaa
     7021 ataattcctt ctactttttg catacaattc ctccaatatt ttttgatata attatattac
     7081 actttttatg attaatttta caaccctcaa tttatccaat tctaatattg taaaattgta
     7141 attttcaaaa gttacgcttt ggtttacttt aatatttgaa ccaagcatat aggatatcca
     7201 cccacctata ctgtcaaatc catcatgctc tatatcaagt ccaaaatact tattaatata
     7261 gttgatagat tcagttccat caactaagta agaacctgct cctattttct taatatgagt
     7321 ctcttctttg tcaaattcat cttgaatttc tccaactatc tcttctagta tatcttctat
     7381 tgttaccaca ccagatgtac caccatattc atccacaact atagcaagtt gaagtttttc
     7441 ctttcttatt ctctcaagag ctttatctat agttaaattt tcagatatat atataatatc
     7501 tctcaatatt tcttctagtt caattttatt ttcatttatc ttttgattgt ataaatctct
     7561 tatatgtaca aaacctaata tatcatcttt attttttctg catactggat atcttgtaac
     7621 accttcctct tttaaaatag ctaatatctt ttcttcagaa tcactttcat atatacatac
     7681 catatcagtt ctaggaacca ttatctctct aattttcttt tcttcaaatt caaaaatatt
     7741 atcaactaat ctttgctctg attcatctat aagtccactt ttataacttt cttctactaa
     7801 tagtcttatt tcgtcacctg tatggggttc atcaacctca tcagcttgtg aatatccaaa
     7861 tggtttaaga aataaatcag tacttaagtt gaaaaaatat attattggat atgttaactt
     7921 ataaaatcct actaataaaa aagatgtact caacattatt ttttctgtat tataaagtgc
     7981 taaagcttta ggaaccaact ctcctattac aacctccatc attgttataa ttaaaaaaga
     8041 aatggaaatt gatatagtat atataatact ttcactaaga ttaaatacac ttactattgg
     8101 taaaattaac tctttaactg tagattctcc catccaacct aatgcaagtg aacaaagtgt
     8161 tataccaagt tgacatgcag aaagatagga atttaagttg tcttttatta ttttacatct
     8221 ttttgcatta ttattacctt cagttatcat ggtatcaatc ctagaactac gaagttttac
     8281 catagcaaat tcacaagcta caaaaaaagc atttatcaaa acaagcatta ctataagtgt
     8341 tacttttaat aactcccgca taaagtattg gttcttataa tgtaagaaat ctataagaac
     8401 tcaaagttcc ccccttatat ttataaattt aatttataat atatattcta tca
//
LOCUS       DAELXX010000145         8277 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.147, whole genome shotgun sequence.
ACCESSION   DAELXX010000145 DAELXX010000000
VERSION     DAELXX010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8277
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.147"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..345
                     /locus_tag="KPH29_003311"
     CDS             <1..345
                     /locus_tag="KPH29_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG1468878.1"
                     /translation="LIKENDIICIEDLQVKNMIRNRKLSRLISDVSWSEFIRQLEYKA
                     NWHGRQIVKVGKFFASSQICNKCGYKNEEVKDLNIREWICPSCNEIHDRDINASINIL
                     KEGLRLITIQNK"
     gene            410..871
                     /locus_tag="KPH29_003312"
     CDS             410..871
                     /locus_tag="KPH29_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG1468879.1"
                     /translation="MQNSVKQEPCDFSHGRFRFGGKIIRRNEKTKFDWLSRDKEQVEK
                     YINDPFCGVVCSCGFFYDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGV
                     LRLRDRYIKLGVKDVTCKLYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
     gene            1336..2550
                     /locus_tag="KPH29_003313"
     CDS             1336..2550
                     /locus_tag="KPH29_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1468880.1"
                     /translation="MQIKIGKVIQRLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEKVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETTNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNIFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLWYANLSLEIKKMLSEDKGAFFSMYVDYMELSLIFLFFNMKEEAKKALYSLKEIL
                     EKYPIHESLDVSQMRFFDKVESKNLYTFNIYTNLLIVLNDDSYNELREEPIFKDVIEK
                     ILDMEKMLKNKE"
     gene            complement(2970..3491)
                     /locus_tag="KPH29_003314"
     CDS             complement(2970..3491)
                     /locus_tag="KPH29_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG1468881.1"
                     /translation="MKFERLRTSENKLYYKAMELYKISFPFHEQRKSYLQTEILKNKE
                     YQFNLIYDKNQFVGIILCWETDNFIYVEHFCIFPEMRNNRYGQRALELLNKKGKTIIL
                     EIDLPIDEISIYRKAFYERVKYKTNNYEHIHPPYHEKFNGHNLIVMSYPEKLSKMEYD
                     KFNHYLRNNVMSF"
     gene            3847..4644
                     /locus_tag="KPH29_003315"
     CDS             3847..4644
                     /locus_tag="KPH29_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBG1468882.1"
                     /translation="MEKREKRIIISSLLSVSILMGLVSIYSILNKEDIILTVKGQEQK
                     VSSFKKTVEELLDEQGVKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            4754..5344
                     /locus_tag="KPH29_003316"
     CDS             4754..5344
                     /locus_tag="KPH29_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBG1468883.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNEETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVREDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFADFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            5410..7107
                     /locus_tag="KPH29_003317"
     CDS             5410..7107
                     /locus_tag="KPH29_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBG1468884.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGISLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     NVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            7321..8160
                     /locus_tag="KPH29_003318"
     CDS             7321..8160
                     /locus_tag="KPH29_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBG1468885.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLVGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
BASE COUNT         3306 a          798 c         1401 g         2772 t
ORIGIN      
        1 ttaataaaag agaacgatat tatttgcata gaagatttac aagtaaagaa tatgattaga
       61 aatcgtaaac tatctcgctt aatttctgat gtgtcatggt cagagtttat tcgtcaatta
      121 gaatacaagg ctaattggca tggtagacaa attgtaaagg taggtaaatt ctttgcaagt
      181 tcacaaatat gcaataaatg tgggtataaa aacgaggaag tgaaggactt aaatataaga
      241 gaatggattt gtcctagttg taatgaaatt cacgatagag atataaatgc aagtataaat
      301 atattaaaag aaggattaag actaataaca attcaaaata aataacttat gtaagaaccg
      361 taggaactac ggggatagcc tgtggagaat tagtaagaca tattttagta tgcaaaattc
      421 tgtgaagcag gaaccctgtg actttagtca tgggaggttc agatttggtg gcaaaataat
      481 aagaagaaat gaaaagacta agtttgactg gttaagtaga gataaagaac aagttgaaaa
      541 atatataaat gacccatttt gtggagtagt gtgtagttgt ggcttttttt atgatttagt
      601 tcaagggtta aaagaaattg aggataaaga aaacttaaaa aaagtgccac ttgatatacc
      661 tatttacata atatctggag ataaagaccc cataggaaaa aatggtaaag gagtcttaag
      721 actaagggat agatatataa aattaggagt aaaagatgta acttgcaaac tttacaaaga
      781 tggaaggcat gaattgttga atgaaataaa tagagaagaa gtttttgagg atataatttg
      841 ttggttaaac aataaaatag aaatattgta ataaaaaaat ataatgatat atttgcttta
      901 aatttattta tatataatat ttgaaaagtg gattatgtat atgcaaagta gaaaagagag
      961 agcctagaag tgattttttg ctataatgcc atttattgaa taggtgggtg ttattataaa
     1021 taaattatta acactcagta ttattatcat tgtatatgga atttatttta tttaaaattc
     1081 cttgaaagtt taaatatagt aagttgtagt aaagagaaaa tctaaatatt taaatgctat
     1141 ttaatgtgaa tctattaatt aggttatact taaacttaat taatagattt tatatttcaa
     1201 gccaatgaga tagggtttta gattaaaata taaccaatgg ttaaattaca gacattttgt
     1261 taactttccc tcgatttact agtaaatatt ttttttatat acttaaatta caacagatgt
     1321 actagggagg tatatatgca aattaaaata ggtaaagtca tacagcgtct tagaaaagaa
     1381 cgaaatttaa ctcaagaaca attagcaaag tttataggtg tgtcaacacc agcagtatct
     1441 aagtgggaga gtggaaattc ttacccagat atagaattat tgcctttatt agcagacttt
     1501 tttaatgtgt ctattgataa gcttttaaat tacaagattg atttaagtga agaaaaagta
     1561 atgaaaattt ataaagaatt agaatcaggc tttgctagaa ttgaaattga tttatcaaca
     1621 gaagaaccaa aagaagaatt tagacaagac ttagaatctg taaaaaagct ttctaatatg
     1681 tatatagaga agtatcctaa aagctattta ttgaagctaa ggatatgttc gctgtatcag
     1741 atgtattcat ataaatttgg taagagtgaa cttaatgaca gagtaaaaga aacaacgaat
     1801 attcttgaag atatagtgag aaatacagat gatatccaaa taaaagaaac agctcttatt
     1861 atattatcaa acgcatattg tatgttggaa gattatgaaa aggcagagtt gtatttaaat
     1921 atgatacata agtcaatagg ggatacaagt gttaatttag ctatgatata tttaaagcaa
     1981 aatagacttg aagaagcaga aatattgctt caaaataaat tgtttagtaa tatatttaat
     2041 ataagcatgg attgtaaggg aataattaat gtatacaaaa atcaatacaa ggaattaaag
     2101 aaaaaattgg agaatagaaa ttttaataaa aatgcaattg agagagaaat ggagtatata
     2161 aaaaataagt tgctgtggta tgcaaatctt tctttagaaa taaaaaaaat gctcagtgaa
     2221 gataaaggtg cattttttag tatgtatgtg gattatatgg aattatctct aattttttta
     2281 ttttttaaca tgaaagaaga agcaaaaaaa gccttatata gtctaaaaga aatattagaa
     2341 aaatatccta tacatgaaag tttggatgta agtcaaatga gattttttga taaggtagag
     2401 tctaaaaatt tatatacttt taatatatat acaaacttgt taattgtact taatgatgac
     2461 agttataatg agcttagaga agaaccaatt tttaaggatg taattgaaaa gattttagat
     2521 atggaaaaaa tgttgaaaaa taaagaataa gtaaaaattt aatataggac ttaaagatta
     2581 atcaaattaa aagaaagtgt ttcaaaataa aaatttgaat cacctttttt gttggtctta
     2641 aaccgttatt tagaaaaata gaggtttcaa aatagaaatc tgaattatct gtattattac
     2701 acaaaaatag aaacctaaat ttataaaaat agaataaagt ttgttatata aaatgtaatt
     2761 ttgtctataa aatctcacat gttttcttaa ctttaagcgt gaaaaagagg atgtatcatg
     2821 aattttttag ttcatgatgc atccttattt gtatgaaatc caatatatct ttattattta
     2881 aacaatggaa aagagtctta tttctatttt gagacatctc ttttatttaa ttatgaagtt
     2941 ttatacagtt caataaatag gaattatatt tagaaactca tgacattatt tcttagatag
     3001 tgattaaatt tatcatattc cattttagat aacttttctg ggtaagacat tacaatcagg
     3061 ttatgcccat tgaatttttc atggtatggt ggatgaatgt gttcataatt atttgtctta
     3121 tattttactc tttcatagaa cgccttgcga taaattgaaa tctcatcaat tggaaggtca
     3181 atttctaaaa taatagtttt tcctttctta tttaaaagct ctaacgctct ttgtccatat
     3241 ctattattgc gcatttctgg aaaaatgcag aaatgttcta catagataaa gttatctgtt
     3301 tcccaacata aaattatgcc gacaaactgg tttttatcat aaattaaatt gaattgatat
     3361 tctttatttt tcaaaatttc tgtttgcaaa taagattttc tttgttcatg aaatgggaaa
     3421 ctaattttat ataattccat agccttgtaa tataatttat tttcactggt tcttaaccgt
     3481 tcaaatttca ctttactcct cctaatcacg aaaattccaa tttatttatg agttcatttt
     3541 accatagtca gtatttgcat acaatgtgtt ttaagttgag tgtcatttct ttcttttatt
     3601 tatgcttata tctcatagac taagagataa ttgcttaaga cataactaaa aactattata
     3661 ttaagctttg ataatctaaa aacagtatat ttatggactt ttctaggtct aaaagtaatc
     3721 ttaataattt tagaagttac aaaaaagtta cataaatttg gaaagctata atatacatat
     3781 ataatataag gttaaaactg taatgaataa tattaattta aaatttgaac atggaaggag
     3841 attattatgg agaaaagaga aaagaggata ataatatcat ctttattaag tgtatcaatt
     3901 ttaatgggtt tggtaagtat atattctata ttaaataaag aagacataat tttaactgtt
     3961 aagggtcaag agcaaaaagt atcttccttt aagaaaacag ttgaggaact tttggatgaa
     4021 cagggtgtaa agtataattc tgaagataag attaatccaa gtttagatac agaactaaaa
     4081 gatgatatga aaataaaagt tgttaaggta actaaaagta aaaaagaaga gattgaaaaa
     4141 attccatttg atacaaagca tgtaaatgat agtaatttgt taaaaggaaa atctaaagtt
     4201 tatcaagaag gtcaagaagg agaaaaaaaa ctagtctata atttaactta ccatgatgga
     4261 aaattagtca aaaaagtctt atcaaaagaa gtaatatcta aggagccaac tacaaaaatt
     4321 ataaaatatg gaactaaaga aaaagtacta atagcatcaa gaggagcaaa tataagagga
     4381 ggcaaacata tgaaagtggt tgccactgca tatgcaggag atacaataac atctactggt
     4441 acaactccaa gatggggtgt tattgcagtt gacccacgtg taataccata tggaacaaaa
     4501 gtatatatac ctaaactggg tatgactttt gtagcagaag attgtggagg tgcaattaaa
     4561 ggtaatagaa tagacatttt tatgaacagt gaaggaaaag cttcaaattg gggaaggaaa
     4621 agtatagata tatatctaca ttaaattagt gaatagaata actttacttg aaggacaaaa
     4681 aagtataaaa tatgaattaa ctaaactgtt agattattct taaaacataa aaatatatct
     4741 aaggggggat aagatgaaaa tagtctgctt aggagacagt ttaacgtatg gatttggagt
     4801 atcacgaagt aattcatgga caaacattgt aaatgaagag actcgattag aaatagtaaa
     4861 taagggtata aatggtgata caacaagtgg tatgttagct aggtttaatg aagatgttgt
     4921 aaaaaactct cctgatatag tttttataat gggtggcact aatgatttta ttgctggtgc
     4981 gggaaatgaa attattaatt ctaatataat ggctatggtg catcagtcat attcaaaaaa
     5041 tattatacct ataataggta taccattaaa accagatatt ccaaatgtta gggaagattg
     5101 gagttgtttt actgatttta atatagtatc tcaaaactta gaatcatata gttattggat
     5161 aaaaaaattt tgtatgacat ttaatactaa tttcgcagat ttttattcag aatataataa
     5221 aaatatggaa ctagaaggat ataaaaaatt atattttgat gggttacatc caaccaaaga
     5281 gggtcataga ataatggcag acatttttat taattcaatt aataagtata tagaagagtt
     5341 atagattaca ttaaaatgct tgtttatgcg tcagttgcaa taaatacata cagtagagag
     5401 gggtttatta tggattatac taaaacttat gaagagtgga taaagggttc atattttgat
     5461 gaagatacaa agctagagtt ggagaatata aaaaataatg aaaaagaaat agaggataga
     5521 ttctataagg atttagaatt tgggactgcg ggtcttagag gaataattga agcaggaacg
     5581 aatagaataa ataagtacac agttagaaga gctacttttg gattggcaaa ctatatatta
     5641 gaaaacacaa caaaagaaga aacaagtaga ggagtagtaa tagctcatga taatagacac
     5701 aagtctagac aattctgtat agaatctgca aatactttgg cagcatgtgg tataaaagca
     5761 tatatatttg atagtttgag aactactcca gaattatctt ttgctgttag aagcttaaat
     5821 gcaatcgctg gtatagtcat aacagcaagc cataatcctc cagaatataa tggatataag
     5881 gtatactggg aagatggagc acaagttatg ccagagattg caaatgcaat aacagagaaa
     5941 gtaaatagta tacatgatta tagtacaata ccaaccttaa cggaagctaa taaaaatttg
     6001 gtagttttgt tggacgaatc acaagatact aaatttatag aagcagtaaa aagccaaatt
     6061 ataagaaaag atttagttaa aaatgtaggt aaaagcttta agatagttta tacacctctt
     6121 tgtggaacag ggaatgttcc aataagaaga gcattgaaag aagttggttt tgaaaatata
     6181 attgtagttc cagaagaaga aaatccagac ccaaattttg cagggcttga ttatccaaat
     6241 ccagaagaga aaaaagcttt aaatagaggt atattactcg caaaagaaaa aggagcagac
     6301 cttgtaatag caactgaccc agattgtgat agagtaggtg tagcagtaaa aactactaca
     6361 ggtgagtatg ctttacttac aggaaatcaa attggtggta tgctgacaca ttatataata
     6421 gaaggattaa aagaaaataa caaattaaaa gaaaatccta caataataaa aaccatagtt
     6481 acatcagaat ttggagcaga tatagctaaa gcaaataatg ttgatgtatt aaatgttctt
     6541 acaggtttta aatttattgg tgaaaaaata aaattatttg aacaaaataa aaatagaagt
     6601 tatgtatttg gatatgaaga aagttatggt tacttagttg ggactcatgc tagagataaa
     6661 gatggtgtag tttcttcatt gttaatatct gaaatggcag cattttacta ttcaaaaggt
     6721 ataagtttat atgatggttt aatagaactt tataaaaagt atggattttt caaagaacag
     6781 actatatctt tgactttaaa aggtatagaa ggtgtagaaa agataaaaga aataatatct
     6841 tattttagag aaaatcaaat tgattatata aatagtatta aagtagttga taaaaaagat
     6901 tataaaaatg gtatagataa tcttcctaaa tctaatgttt taaaatactt tttggaagat
     6961 gaatcttggg tagctataag accatctgga acagaaccaa aacttaaatt ttacatagct
     7021 gtaaaaggag ctagtgatat tgaagcagat aaaaagattc aaggtttgaa aaagtatata
     7081 gatgacatgg tcgaaaaatt aaaataaaat attattattt tataagataa ttttgtaata
     7141 atatttattt agctgaaaat tataatttac tttcattgag tagaatatgg aaatgttagt
     7201 agaattgtta ttttcagctt ttatttgtgt tatttttact taaataatta tattatagaa
     7261 tgaatctgag tataattaat attgtataat gaaattaatt tgatagaaaa ggggatattt
     7321 atgtttggta tagataagaa attgattgaa tatataattg caaaatccaa ttacagtgaa
     7381 ttaagttcaa agtcagcaga agtatctttc ttcttaatgt tgtccatatt tcctttttta
     7441 atatttacaa taagttctat agcttatata ccgattcttc atttaaataa gtatatagct
     7501 ttgtttagaa atatgatgcc agaaggtgct tttgctgtgt tatcttcaat catagtttct
     7561 gcaatagaca atagaaacct gaaattttta gcagtcagtt ttgtacttac tatgtggaca
     7621 ttttcaagag cagtaaaagc tcttattaaa ggaatgaata gagcctataa agttaaagaa
     7681 actaggtctt tttttaaaat tttatctata tcatttttat ttacaataat gcttttggtc
     7741 cttatttttt tatctatgat atttttagtt tacggggaaa aaataggtta ttttattttt
     7801 aatcttgtag gattggatga gatatttata aaaatatggg atattctaag atacactgta
     7861 ggtataataa caataatagt gatatttaca cttttatata aatacactcc aaataaaaag
     7921 ttaactatta aagaatctgc gccaggtgct atttttgcta cttttgcttg gtttttagta
     7981 tcatttttat attcttatta tacaaattac tatgctaact atgaagtaat ctatggaagt
     8041 atagctgaaa taattgtact tatgacctgg atgtatttta gtagttggtc tatcgttatt
     8101 ggctatgagg taaattcaag attgtatttt agaaagataa gacatgaaat gttaaagtaa
     8161 agtatttaaa ataaaagaga gtatttagta ttataaatgt aattgaatta ataaagaaat
     8221 attgacattt aagttaaaaa gtctagaatt tttctgggct ttttttattt ttagtat
//
LOCUS       DAELXX010000146         8099 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.148, whole genome shotgun sequence.
ACCESSION   DAELXX010000146 DAELXX010000000
VERSION     DAELXX010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8099
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.148"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..996
                     /locus_tag="KPH29_003319"
     CDS             <1..996
                     /locus_tag="KPH29_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HBG1468886.1"
                     /translation="SGYCIFHKENKSDEEIQLMMDTIHKEEISEFNGFVFENEFNAEE
                     ILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTEFRDKVLFNGCIFLENCDFNRT
                     IFSKNYINDRIFEKVKFKGPDLVVNKVENFPRMDGIIFSMCTKFVLKNVEYGKSEYEH
                     GKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMKRSNYPTFSDYLVEKFFDQIAR
                     YTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNSTLVALDINNIGDYSLSEIFKM
                     YMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFFGVTIGAIWASVIIKRMIR"
     gene            1100..2284
                     /locus_tag="KPH29_003320"
     CDS             1100..2284
                     /locus_tag="KPH29_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG1468887.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADGKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHICKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMVVNKNEKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGMKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGVIKYYTSSGDLLGSVDIVSNNSIDNVSLKTKLKMKF
                     AN"
     gene            2491..3204
                     /gene="rpsB"
                     /locus_tag="KPH29_003321"
     CDS             2491..3204
                     /gene="rpsB"
                     /locus_tag="KPH29_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBG1468888.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            3283..4194
                     /locus_tag="KPH29_003322"
     CDS             3283..4194
                     /locus_tag="KPH29_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBG1468889.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            4279..4983
                     /locus_tag="KPH29_003323"
     CDS             4279..4983
                     /locus_tag="KPH29_003323"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBG1468890.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            5009..5566
                     /gene="frr"
                     /locus_tag="KPH29_003324"
     CDS             5009..5566
                     /gene="frr"
                     /locus_tag="KPH29_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBG1468891.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDTLLSKKEKDIMEV"
     gene            5569..5772
                     /locus_tag="KPH29_003325"
     CDS             5569..5772
                     /locus_tag="KPH29_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468892.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            5801..6535
                     /locus_tag="KPH29_003326"
     CDS             5801..6535
                     /locus_tag="KPH29_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBG1468893.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            6548..7327
                     /locus_tag="KPH29_003327"
     CDS             6548..7327
                     /locus_tag="KPH29_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBG1468894.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
BASE COUNT         3342 a          807 c         1507 g         2443 t
ORIGIN      
        1 tctggatatt gtatctttca taaagaaaat aagtctgatg aagagataca gttgatgatg
       61 gatactattc ataaagaaga gataagtgaa tttaatggat ttgtttttga aaatgaattt
      121 aatgcggaag aaatattaac ttacaattat aaaatactgg atttttctga atccatattt
      181 aaacaaaaag caaattttaa aaaatatatt tttaaaaaga atattatttt taactataca
      241 gaatttagag ataaagtttt atttaatggt tgtatattct tagaaaactg tgattttaat
      301 agaaccatat ttagtaaaaa ctatataaat gaccgtatat ttgaaaaagt gaaatttaaa
      361 ggacctgatt tggtagttaa taaagtagaa aattttccac gaatggatgg aataattttt
      421 agcatgtgta caaaatttgt gttaaagaat gttgaatatg gaaaaagtga gtatgagcat
      481 ggcaagataa attatagaat agctagaaat caagcaacaa aaattggaga atatgagatg
      541 ataggatttt actattataa ggaaagaatt tatagtagta aaataatgaa acgttctaat
      601 tatcctactt tcagtgatta tttagttgaa aaattttttg accaaatagc acgttacaca
      661 acaggttatg gagaaaagcc atggaatata ttattagtga ttatagcaat aataagtgtt
      721 tttgctttat tatacttatt tgtaggcata gaaagttcaa atagcacact tgtagcttta
      781 gatataaaca atataggtga ttactcacta agtgagattt tcaaaatgta tatggattta
      841 tggtatttta gcatggctac atttagtaca gtaggatatg gagatatggt tgctacaagt
      901 ttgattggga aagcattggc aggtatagaa gtattttttg gagttacaat aggagcaatt
      961 tgggcatctg tgattattaa aagaatgata aggtaatatt tgtgtaaata tggataggta
     1021 ttaattacta gatataaatt ggaccaaata gggaaaattt aaatataaat atacttaata
     1081 atccaagaag gtgaaaaatt tgaaaaatgc actaaaaaaa attatatcat tagccatgat
     1141 tctagttttt ataataccaa attattcata tgcagatggt aaatctgtag acaagtattc
     1201 aaaaacatct atacttatag accaagaaac aggtagagtt ttatatagta aagaacctga
     1261 tatgaaggta ccattagcta gtttaagtaa gatgatgact tttttacttg caatagaagc
     1321 tattgaaaat aaagaagtaa aagaaactga taaggtaaaa atagacaaat ctattgcatc
     1381 agtaaaaggg tcaagttata aacttaaaga tggtgaagaa gtgccactta tagagcttat
     1441 gagaggtctt atgattgtat caggaaatga tgcggcaatt gctatagcaa aacatatttg
     1501 taaaacgaaa gaagcatttg tagatagaat gaataaaaaa gcaaaagaaa taggaatgag
     1561 taatacacat tttctaaatc caaatggtct tccaatatac gatttaagca accccaaaaa
     1621 accagcaaaa gaaaatatct ctactgcaaa agatatagct attcttggaa aatacatgtt
     1681 tgaccattat gaaaaacaag taacagctat aactgatatg gagacatata caaattcaga
     1741 tagaagtttt gaaaaaagca atacaaatgc attattaaga attataccag aagtagatgg
     1801 tataaaaact ggatatactg gcaatgctgg atattgctta tcattttcta tggttgtaaa
     1861 taaaaatgag aaaaatgaga aaaaacgtag agtaataggg gttgtattag gtactaacca
     1921 taaaaataaa agaacctctg cttcattagc tctactgaaa tatggaaaag aaaatttcaa
     1981 tatgaaaaaa gtaattgata aagatagctt tattggtaag aaatatataa aaggaatgaa
     2041 agagttagag gttacattaa aagcaaaaga tgaactttat acaatattaa aagatgatga
     2101 aagtataaaa tcagaagtta agattaaaaa tatagagtac cctgttaaaa aaggtgatac
     2161 tatgggtgtt ataaaatatt atactagttc aggtgactta cttggaagtg tagatatagt
     2221 aagtaataat agtatagata acgtatcatt aaaaacaaaa ttaaaaatga aatttgcaaa
     2281 ttaaattcaa aaaatggtat aaaatacaca aaaaaatata aaaatagaaa atatggaaaa
     2341 aatattgtta tttcttcgcc tctgtgatat actactagag gtgaaaaaat aaaataaatc
     2401 acacacagca ttgggatcct taaaaggtgc ctattacatg gttttttagg gagaagatca
     2461 agctggaggt ataaaaccag gaggtaagat atgtcagtaa tatcaatgaa acaattatta
     2521 gaagcaggtg ttcactttgg tcaccaaaca agaagatgga accctaaaat ggctaagtat
     2581 atattcacag agagaaatgg aatatatata atcgacttac aaaaaacagt taaaaaagtt
     2641 gaagaagctt acaagttcac taaagaagta gctgaaacag gaaagccaat cttattcgta
     2701 ggaactaaaa aacaagctca agatgcgata aaagatgaag ctgaaagatg tggaatgtac
     2761 tttgttaatg aaagatggtt aggtggaatg ttaacaaacc acaaaactat aaaaacaaga
     2821 ataaataaat taagagaatt agaaaaaatg gaagaagaag gcgtatttaa tgttcttcct
     2881 aaaaaagaag ttataaaatt aagagctgaa aaagaaaaac tagaaaaata cttaggtgga
     2941 ataaaagaca tgcctgaatt accaggtgca atgttcgtag ttgacccaag aaaagaaaac
     3001 atagctattc aagaagctca tagattaggt ataccagtag ttggtatagt tgatactaac
     3061 tgtgacccag aacaattaga ctttgctata ccaggaaatg atgacgctat aagagccgta
     3121 aaattaataa ctggtgctat ggcaacagct gtaattgaag gtagacaagg tgctgaggaa
     3181 gaagtagctg aagaccaaga ataataattt gacaattaaa tggtaagggt cgcccttacc
     3241 atttataata tggaatagaa taagtaggag gaaacttaaa aaatggctaa tataactgct
     3301 caaatggtaa aagagttaag agaaagtaca ggcgcaggaa tgatggactg caagaaagct
     3361 ttacaagaag ctgaaggaaa catggaaaaa gcagtagatt tattaagaga aaaaggatta
     3421 tcaaaagctg ctaaaaaagc tggtagagtt gctgctgaag gtctagttgc aatagaaatg
     3481 aatgatgata atacagttgc ttctatggta gaagttaact cagagacaga tttcgttgct
     3541 aaaaacgaag acttcaaagt gttcgttaaa gatgctgctt gtatggcatt agctactgat
     3601 aaagaagata tagcttcttt attaggtgaa actcataaag agggaataac tttacaagaa
     3661 gttttaaata atagagttgc aaaaataggt gagaaattag actttagaag atttgctaaa
     3721 gttgtaacta atggacaagt tgctggatac attcacggtg gtggaaaaat aggtgttctt
     3781 gttgaaatgg aaacagaagc tagagatgct aaagttttag aattaggtaa agatgtagct
     3841 atgcaagttg ctgctatgaa tcctaagtat gtttcaagag atgaagtaga tgctgaatac
     3901 atagcacatg aaacagaagt attaactcaa caagctttaa atgaaggaaa accagctaat
     3961 atagttgaaa aaatggttaa aggaagatta gaaaaagaat taaaagaggt ttgtttatta
     4021 gagcaaactt tcgttaaaaa tccagatata actgttaaac aattagttgc tgatgttgct
     4081 aaggcagtag gttctgatat aaaagttgtt aaagttgtga gattcgaagt tggtgaaggt
     4141 atacaaaaga gagaagaaaa ctttgcagaa gaagttgcta agcaacttaa gtaataacca
     4201 aagagagaac acgcaggtgt tctctttttt aaaaaataga aaaaattgga tatgaaggat
     4261 tgaaatagga ggtttctgat ggataaacca atgtacaaaa gggtattgtt aaaattaagt
     4321 ggagaagcat tagcaggaga aagaggtttt ggtataaata atgatgtagt taatgatatt
     4381 gctattgcta taaaaaaaat acaagaaata ggtgttgaag tagctgtagt agtaggtgga
     4441 ggaaacttct ggagaggcag aaccagtgaa ggaatggata gaactactgc tgactatata
     4501 ggaatgttag ctacagttat gaatgcaatg gcgctacagg atgcactaga aaatatagat
     4561 gttgctacaa gagttcaaac tgctatagac atgagacaaa ttgcagaacc atatataaga
     4621 agaagagcag ttagacactt agaaaaggaa agagttgtta tatttggtgc aggaacagga
     4681 aacccttact ttacaacaga tacaactgct gctttacgtg ctgctgagat ggaagcagaa
     4741 gttatattgc tagctaaaaa tgttgatgca gtatatgaca aggatccaaa agtacatgca
     4801 gatgctaaga aatttacaga attaagttac atggaagtta tacagaaaga attaaaagta
     4861 atggattcta cagctacatc tttatgtatg gataataaaa ttccaataaa ggtatttgag
     4921 cttacaacag agaatataat aagagcagta aaaggcgaaa atataggaac tactgtaaaa
     4981 taatgaagaa tgaaggagga gtaaaattat gaaactggaa atacataagc aattagaaga
     5041 gaaaatgaat gggacaattg atgctttaaa gtttgaattt ggaactataa gagcaggaag
     5101 agctaatgca caaatgctag acaaaataag agttgattat tatggaactc caactccaat
     5161 aaatcaaata ggtgctatat ctgtaccaga accaagaata ttaatgatat ctccttggga
     5221 taaatctgct atgcatgaaa tagaaaaagc gatagctaac tcagacttag gattaaatcc
     5281 atcaaacgat ggtgaggtta taagactttc tgtacctgct ttaacagaag aaagaagaaa
     5341 agaattagct aaaaaagcta gtaaagctgc agaagaattt aaagttagaa taagaaatga
     5401 aagaagagac gccaatgaga aaattaaaaa gatggaaaaa ggcggagaat taactgaaga
     5461 tgaattaaaa aaagcacaag atgaagttca aaaaatgact gataaattta taaaagaaat
     5521 agatacatta ttatctaaaa aagaaaagga cataatggag gtataatatt gaaaaaatat
     5581 tataaccttt taggtcttca tgtagatgag gtaaaacaat attttgatga gcaaaatata
     5641 aattataccg taaaatctat tgaaggtaaa aaggataaag aaaaacttgt agttcctaga
     5701 gttataaaaa tatctgagat aggtgatagt gtagaactga taatcacata cttttctgac
     5761 tccttagttt aaggagtcag aacaatattg gaggcaaaat atgaataaca acattatata
     5821 tgatatagac ttaaataaca tacctactca tattgctatt ataatggatg gaaatggaag
     5881 atgggcaaaa gctagatttc ttccaagaac tgcaggacat aaggctggag tagaaactat
     5941 aagagatata gtaaaagaat gctcaaaatt aggtgttaag cacttaacat tatatgcatt
     6001 ttcaacagaa aactggaaaa gaccgaaact tgaagttgac acacttatga acttattatc
     6061 tacatactta agaaatgaaa tagctgaact tcaccaaaac aatgtaaaag taactgctat
     6121 aggagatata agtgctttac caaaaacttg tataagagaa ttaaactcag ctaaggaact
     6181 gacaaaaaac aatacaggag ttaacttaaa tttagcatta aactacggaa gtagagcaga
     6241 tataaaaaat gctcttatag atatagtaaa aaattgtgaa agtggtaaaa tagatataaa
     6301 caatattgat gaagatatta taaaaaacta tttaagcaca aagtctatac cagatccaga
     6361 cttggtaata agaacaagtg gtgaacaaag attgagtaac tttttacttt gggaagttgc
     6421 ttattctgag ttttacttta ctgatataca ttggcctgat tttaaaaaag aagaactaca
     6481 gaaggctata tacgtatatc aaagaaggga tagaagattt ggaggactta agtaaggaga
     6541 ttattttatg cttacaagaa ttattgcttc actagctctg gttccattat ttctatttgt
     6601 tgtatatgga ggtattcctc tatatatagc tgagacagca atagtttata ttgcattgca
     6661 tgagttttat aaggcattta aaataaaaga tgtacatcca atattcatta taggatattt
     6721 attttctatt tatttagctg taaaaaatat ttttaattta ccattagaat atacttatgc
     6781 agtaattttt attttatttt tagcaagtat aatttatatg ttgatgggca aaaacaatgt
     6841 tatagatgtt tcgattacgt ttttaggagt tttttatata ggagtatttt tagattttat
     6901 aattataact ataaatggat ttgaaaaagg tagtatatat gtatggctta tatttgtgat
     6961 atcttttatg acagatatat ttgcatattt tagtggttat ttacttggaa aacataagct
     7021 aattcctaaa gttagtccta aaaagactat agaaggagct attggaggaa taataggaag
     7081 tacactttgt tgtattttat ttggatattt atttggcatt gatttgcttc aattagcgat
     7141 aataggaagt ataggaagtg taatagctca attaggagat ttatttgcat cttctataaa
     7201 gagatatgta gggataaagg attatggaaa gataatacct ggtcatgggg gtatattaga
     7261 tagatttgat agtgttatat tagttgcacc ttttgtatat agtgcgatta aattttttat
     7321 tagataaaga ttggttggat ttaaaagagc aatctataat tgctagtaat gaaaatagct
     7381 atgtaacttt taaatgcaga gatattctgc agttattagt tacatggcta ttttcattgt
     7441 tcaaaaattt agaagacctt ggggaatttt ataagaataa gtattacttt ataatagtat
     7501 actatatttg tagtgagtat attttttata ctataactat cattaaaaaa tatttttttg
     7561 tttaataatt tcgatatttt tagctttatt atatatttat aatatatatg tataaatgta
     7621 actaatttat tatccattta taaattttat ttttaaatat aattaataag aaataagtaa
     7681 attattggtc ttattgcaca aaaatagata taatttaagt aatataccaa aaaaaaggaa
     7741 tatatttaca taatatgata aaataaattc aaagagttaa attgtaggta aatagtattt
     7801 taattattta ttaaaatata gattatgtat atgccaaatt taaaaagaga cataaagaca
     7861 ttatacaaat atattattgg ggtgatattt tataagaaaa gaagtgatgc agtaaattaa
     7921 ttgagcaatt taaaaatgat ttaatgatag tgttgatgaa tgtttaaaaa gaaattttag
     7981 aaaaacgtat attgctgttt gtgttggagg tgatgaattt gtataattta tagggtataa
     8041 atgattaaac tacttaagat aaatacatat cactgtaaaa tattgataat catttttta
//
LOCUS       DAELXX010000147         7944 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.149, whole genome shotgun sequence.
ACCESSION   DAELXX010000147 DAELXX010000000
VERSION     DAELXX010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7944
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.149"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            167..808
                     /locus_tag="KPH29_003328"
     CDS             167..808
                     /locus_tag="KPH29_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBG1468895.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
     gene            832..2085
                     /locus_tag="KPH29_003329"
     CDS             832..2085
                     /locus_tag="KPH29_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG1468896.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEKENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLREIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            2131..2781
                     /locus_tag="KPH29_003330"
     CDS             2131..2781
                     /locus_tag="KPH29_003330"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBG1468897.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGVDVNNEKEVIQIAENSDIDFKDNNIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKSSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            2832..3428
                     /locus_tag="KPH29_003331"
     CDS             2832..3428
                     /locus_tag="KPH29_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBG1468898.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            3441..4280
                     /locus_tag="KPH29_003332"
     CDS             3441..4280
                     /locus_tag="KPH29_003332"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBG1468899.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFNNSKKYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            4525..5679
                     /locus_tag="KPH29_003333"
     CDS             4525..5679
                     /locus_tag="KPH29_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic efflux protein"
                     /protein_id="HBG1468900.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNPKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKFYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            5985..7682
                     /gene="ade"
                     /locus_tag="KPH29_003334"
     CDS             5985..7682
                     /gene="ade"
                     /locus_tag="KPH29_003334"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBG1468901.1"
                     /translation="MGNLKRQCDVSSGREKADIVLKNGTIINVFTEELITGDVAIVGD
                     TIVGIGDYKGNVEIDCSNKYISPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADP
                     HELVNVKGASAIDFLLESTKDIPLNVYIMVPSSVPATSFETNGVGKFSAKDMESYVNN
                     PRILGLGEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDH
                     ECITFDEVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDI
                     EKQGHIRWNIKCAIDLGMEPVRAIKVATYNSAKAYGLRKIGAIGAGYKADIVVLNDLD
                     KMEVDSVYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEI
                     VPYQILTNKVYESLPCADGYFVPNKEYSKLCVVERHRMTGNVAIASLKGFGIKNGAIA
                     TTVAHDSHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFT
                     AEEVQEITDNMLEIARKMGVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
BASE COUNT         3217 a          795 c         1419 g         2513 t
ORIGIN      
        1 ctttaacagt acaataattt gcagaaatac aaatgagatt tattgtaaat aatatgttag
       61 attagattat aaaatagaaa caaagtaaag tattatatga tataattaag ttttgagaag
      121 tgataattta tatttaataa aaaataggca tatattagga ggacatatgg gaaatacttt
      181 ttatatagta agacatggac aaacggattg gaatatattg ggaaaaacac aaggtcatgg
      241 taattctgac cttacgcctc aaggtataga acaggcaaaa gaactttctg aagatattgg
      301 caagtattca attgattata tttttagtag cgaccttggg agagcaatgc aaacagctca
      361 aattttagga gataagctca atattgaagt tcaaaaaact gaggcactta gagaaatggg
      421 attcggagtt tgggaaggcc ttttaataaa agaaattcaa aaagattatt cagatatata
      481 tgcaacatgg agaaatgaac cacacttagt aaatatacca gaaggcgaaa ctcttaagat
      541 tataaaggaa agagtagatg cttttataaa ggaactaaat gaaaaatatg ataataaaaa
      601 tataattctt gttacacatt caattacatt gagggttatg ttgttgtcat ttttagaatc
      661 aggtatggaa aatatatata gaattaagca agataatact gctttaaata tagtagaatt
      721 taaagattat ggacctgtta tagtaaaaat gaatgataca agtcatataa aaaatcatgt
      781 aaagataaat aattctgcac tggagtagga atcaattata aggagaaatt tatgagtaaa
      841 gttatagtag ttggtggagg agcttctgga atgatggcag ctttgtcagc ttcaaaaaac
      901 aataatgaag taatattagt tgagagaaat ggagaattag gaagaaaact tagggcaact
      961 ggtggcggta gatgcaattt taccaataat agagagatag aagatttttt tgataaagtt
     1021 gtcagtaata aaaaatttct ctatagtagt ttctatacat ttacaaataa agaccttatt
     1081 tcgtattttg aaagtagaaa tttggaatat aagattgaaa aagaaaatga ccacaaagta
     1141 tacactaaaa atgataaatc tatagaagta atagaagtat taaataaaga tttactcaat
     1201 cacaatgtaa agataatgta taataagaaa gttatagata ttataactga agaaattgct
     1261 ctaaaagatg attctaataa ggacaagtca aagtatctaa taaaagggat tattttagat
     1321 aatggagata agatactagg agacaaggtt attatatcaa ctggaggagt gagttattca
     1381 aagacgggtt cagatggttc aatgtataag attcttaaaa agcatggaca tacattaaac
     1441 aaactatatc ctgctttagt tccacttaca atagaggaaa aatggattaa agatttacaa
     1501 ggaatatcca tgaagaatgt ggaaatatct tgtaagatta agaaaaggaa aatatcaaaa
     1561 tctggagaca tgctatttgc ccattttggg ataacaggac cttgtgttct tataatgtct
     1621 tcttatatta acaagattat tgaaaaggaa aaggtagaat taaatataga tttcttacca
     1681 aatttaagta ctgatgaaat ctctagtata atcagagcat ttccaaataa aaatgttcta
     1741 aataatctta agcaaatact acctcaaaat ttcttaagag agattttttc tctattatct
     1801 ttagttgata aaaaggcaag tgatttgtct aaggcagatg aaattagaat tattgagtat
     1861 ataaaaaata tgaagctaac ttgtaatggg actacaggta taaacactgg tatggttact
     1921 tctggtggta tttcagttaa agaaattaat tcttctacta tggaatctaa attagttaag
     1981 aacttatttt ttacaggtga agtaattgat atagatgcag aaactggtgg atataattta
     2041 caaatagcat tttcaactgg atatcttgcg ggtataagtg tataaatata attttttaat
     2101 aattaaatat gaataaatag aggtgtatat atgggtaatt tagttatagc tgtagatggt
     2161 ccagcaggag ctggaaaaag tacaatagcc aagatagtag caaaaaagct aaatataaat
     2221 tatatagata caggtgctat gtacagagca gttacttata agtgtttaaa aagtggtgtt
     2281 gatgtcaata atgaaaaaga agttattcaa atagctgaaa attcggatat agattttaaa
     2341 gataacaata tatatttgga caaagaagtt ataaatgaag aaataagaac aatagaagta
     2401 agcaataatg tttctaatgt agcaaaaata aaagaagtaa gacagttgat ggttgaagtt
     2461 caacgaaaaa taggaatgaa aagttcagta atactagatg gtagagatat aggttcatat
     2521 gttttcccag atgcagatta caaattcttt ttagtagcta ctccagaaga aagaggaaat
     2581 agacgctata aagaactttg taataaagga tataatacta ctttagaaga agtaattgag
     2641 gatataataa gacgagatga aattgattca aatagagaat ttgcaccatt ggtaaaagct
     2701 aatgatgcac tagaaataga tactacagga aaaactatag aagaagttgt agaagaagtt
     2761 gtatctaaaa taaatcttta gttttttatg catcataatg aaaataaatg tagtgttgaa
     2821 gggagataaa cgtgaatttt tatagatttg taataaatat ttttaagggt ttttctaaaa
     2881 tattttttaa atatgaagtt ataggtgcag agaatattcc agatagagga aatatagtaa
     2941 tagcatcaaa tcataaatcg aatcttgacc ctatattctt ggctgctgcc atagaaaata
     3001 gggaaatagc tgcaattgca aagaaagaat tgtttaaagt aaagccattg ggatttatat
     3061 taaaaaaatt gcatgttatg cctataaata gagaaaaacc agatgtatct acaataaaaa
     3121 ctattttaag gtcagttaga gatggttatg tattaggaat atttccagag ggtactagaa
     3181 taaaaggaga ttcttttggt aaggctaagg caggtctttc tgtatttaca ataaaaagta
     3241 aatctaaagt tgttccagta tcaataatat ctaaatataa actttttagt aaagtaattg
     3301 tatatattgg agaaccaata tcatttgaag aacattttaa ggaaaaattg agtaatgatg
     3361 accatgaaag gatatcacaa gagattttag aagtgataaa acaaaattac tttaaatatt
     3421 caaaataagg gggtaaagtt atgaacataa aaatagcaaa aaatgctggc ttttgttttg
     3481 gagtaaaaag agcaatgaaa atggcatggg atgaagttga aaaaaatgat tctggcatat
     3541 atgctttagg tcctcttatt cataacaagc aagcagtagc aaaatatgaa gaaaaaggtc
     3601 ttaagactgt gaatgaaatt gatacaatac caaatcatga aaatatgata ataaggtctc
     3661 atggagttcc tgaaaatata tataaggaag ccaaagataa aaaattaaaa attgtggata
     3721 ctacatgtcc atttgtaaag aaaatacata cagtagtaag tgaatatcat aataaaggct
     3781 atgaaataat agttattgga gatatgaagc atccagaagt tataggtata aatggatggt
     3841 gtgaaaattc agctataata ataaagacct tagaacaaat ggaaaatatg gaatttaata
     3901 actccaaaaa atattgtctt gttgcacaga ctacaataaa tccagagttg tatataagta
     3961 tagttaataa actatcagat aaattagaag aaatagtatt taatgataca atttgttcag
     4021 ctacaaaaac aagacaagaa tcagctaaag agttagctaa agaagttgat tgtatgatag
     4081 ttataggagg taaacacagt tcaaatactc aaaagttagt aaaggtttgt gaagacttag
     4141 taccaacttt tgcaatagag actaaagatg aattggatgt aaatacgctt aaaaaatata
     4201 aaaatttggg tattacagca ggggcatcta cacctaattg gataatagaa gaagttgtaa
     4261 cattccttga aaatttataa tttgataggt aataaaaata ggtggacatt gttataattt
     4321 atcataatat ccacctttga tttcatatta catgtggtaa atagataacc taatatcaaa
     4381 aagtgtgtat cttaagatga aaaactaaga gtataagatg cgcacttttt aatattaaaa
     4441 attcaaaaga aatattgata ttgattatca atagaaaata gaatataata taattgttgc
     4501 aaaattagtc taggaggtaa attaatggaa atgttgatat ccgcttctga agaggcgttt
     4561 ttacatgtag gttctatgat tggatttttt attttgttat ttggatatat taattataaa
     4621 actagtggaa attttactaa tatcatatca aaaaacagaa aatttcaacc cttaataggt
     4681 gctttaattg gagctatacc aggttgtggc ggaagtctgg caataatgcc attgtatata
     4741 aatggaaaat taagctttgg agcaataatt gcaagtttaa tagctagtat gggagatgca
     4801 gcatttgtac ttatttcatc aaatattaaa atgtattttt ttgtcactat agttagtaca
     4861 attacaggaa taataacggg acaattagtg gattacttta aactagaaga aaaattaggg
     4921 ttaaaaaacc gtagaaaatc agataaatat gatataagta aaaataaccc taaagaaaat
     4981 aaagaacaac ataatcatga tgaaataatt ttagacactc tagctaaatc tcatggaaat
     5041 acaaatagac ttgcatttat aataacacat ggaaagggat ataaatttta tataggaatt
     5101 atagtaatcg gttttatatt tatgtcactt gctcactctg gacttaatct tcctataatt
     5161 gagaagttac attcattgga agaagtaata gcagtgattg gaatattatt ttcaataatt
     5221 tatatgtggt gttttaaaaa ggtatttaaa aattctaatc aaaaagagga agaaaataaa
     5281 aagatttctt tgcgagaaat gcttatccat tctgttggag aaatatcatt tgtaattaca
     5341 tggattttta ttgcatatct tatttacgat ttgataatat taattttagg tggagatgaa
     5401 tacttagtta aactagtatt atcaacaggt gtaataagtg tatttatagg tgcaggtctt
     5461 ggacttattc caggttgtgg gatacaaatt gtgcttatgt cattttattt gaagggaact
     5521 ataccacttg gtgcagtgat agccaactct atttctcaag atggagatgc acttttccca
     5581 cttttagcaa tggataaaaa atcttcttta tgggctatga ttattactac tataccagca
     5641 atacttgtag gtatgatagt ttatgcattt ttaggttaaa tataattacg aataaagaag
     5701 tattaaaaaa ataataaagc acataaattt atttataaga atagtgaaga ttaaatataa
     5761 aaataaaaaa ttcaatatga attaaattat tgcttaaata ttaatgagaa catgggagat
     5821 aatattattc ctcatgttct ctctatttaa ttaaaggaat tatggaacaa atttcaagtg
     5881 atactttcta tcaaaactca tatataatat atattaataa attaaactat aatacgtaat
     5941 acagccagta tattgatata aaaaacttag gaggagttgt aaaaatgggt aatttaaaaa
     6001 gacagtgtga tgtttcaagt ggaagagaaa aagctgacat tgtactaaaa aatgggacaa
     6061 tcataaatgt atttacagaa gagttaataa caggagatgt agcaatagta ggagacacta
     6121 tcgttggcat tggcgattac aagggcaatg tagaaattga ttgtagcaat aaatatatat
     6181 cacctggctt tattgatgca catatgcata ttgaatctac aatggtaatg ccaattgaat
     6241 tatcgaaaaa attattaaaa tcaggtacga caactatcat agccgaccca catgagttgg
     6301 tcaatgtaaa aggagctagt gcaatagatt ttttattaga atcgacaaaa gatataccac
     6361 ttaatgtata tattatggtg ccttcatccg tacctgcaac aagttttgaa actaatggag
     6421 ttggtaaatt tagtgcaaaa gacatggaga gttatgtaaa taatcctaga attttaggtt
     6481 taggagaggt aatgtgtttt aatgatgtta taaattctga aaatgaaatc ttggataaac
     6541 tagagctttt taaaaataaa gttgtagatg gacatgcacc taatataaat ggtaaatcac
     6601 ttcagacata tgtatgtgca ggaatagaaa atgaccatga atgtataact tttgatgaag
     6661 tatatgaaaa attgagagca ggattaaaaa ttttaataag agaaggaagc gcagcaaaaa
     6721 atttaaagtc tatagtaagt ggtatgctaa agcataactt gccaattgaa gaatttatgt
     6781 tttgcacaga tgataagcac ttagatgata tagaaaaaca aggtcacata agatggaata
     6841 taaaatgtgc gattgattta ggtatggaac ctgttagggc tataaaagtt gcaacttata
     6901 attctgcaaa agcatatggg ttaaggaaaa ttggagcaat aggtgcgggt tataaggcag
     6961 atattgttgt attgaatgat ttagacaaaa tggaagtgga tagtgtatat aaagatggta
     7021 atttggtaaa cgaagagatg ttttctaatt acaattatga gattaaagat aaagaattgc
     7081 ttaatacagt caagtttaag tatattaata aagaaaaaat acagttaaaa gtaagtgaaa
     7141 aaaattatgt aatggagatt gtaccttatc aaattttgac aaataaggta tatgaatctt
     7201 taccatgtgc agatggatat tttgttccaa ataaagagta ttcaaaactt tgtgttgttg
     7261 agagacatag aatgactgga aatgtagcaa ttgcttcact aaaaggattt ggcataaaaa
     7321 atggagcaat tgctactact gtggcccatg attctcataa tattatagtt gcaggggata
     7381 atgatgatga tattctagta gctataaatt acttaaagga aatacaaggt ggttatgtaa
     7441 ttgtatcaaa tggaaaagtt cttgcacacc taagtcttca agtagctgga ttaattagta
     7501 cttttacagc tgaagaagta caagaaatta ctgataatat gttggagata gcaagaaaaa
     7561 tgggagttcc agagtatgta gaccctttta ttacactttc ttttatggca cttccagttg
     7621 ttccacaaat aagacttaca gatttaggtc tatttgatgt tgaggaattt aaatttatat
     7681 agataatata aaagtaataa taaatgaggc tttctcaaaa tagggataag tctcttttta
     7741 ttgttgaaat gatgaaaata tatttgattt catacaaaaa agagcatcta atgaactaaa
     7801 aattttattt ttagatatcc ttattttaat taatctatca tttaaccttg ttttaagcat
     7861 agatatctta ataaaactat tttcagtcta ttttagaata taaatacatg aaaattatat
     7921 atttatgaaa ctttatacat aaaa
//
LOCUS       DAELXX010000148         7833 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.150, whole genome shotgun sequence.
ACCESSION   DAELXX010000148 DAELXX010000000
VERSION     DAELXX010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7833
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.150"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(325..2604)
                     /locus_tag="KPH29_003335"
     CDS             complement(325..2604)
                     /locus_tag="KPH29_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG1468902.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITEKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYIARSNGNIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSSINNMKSNIKNFKSGSLSYSMFGKGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRESIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFAQVCKNFKRWENENFPTFPISINLSRVHLENPGFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
     gene            complement(2875..4329)
                     /locus_tag="KPH29_003336"
     CDS             complement(2875..4329)
                     /locus_tag="KPH29_003336"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBG1468903.1"
                     /translation="MKLNDIIQGLDIINVKGELNIDINNVQYNSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVEIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            4493..5362
                     /locus_tag="KPH29_003337"
     CDS             4493..5362
                     /locus_tag="KPH29_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG1468904.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDATSDLEK
                     CFKIATENKCNRLRLNIHDIESIKSFIEQINISESNTEFGNEILRNALFIQLMIFLNR
                     IFLKNENMESTEDVFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGYLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
     gene            complement(5576..7648)
                     /locus_tag="KPH29_003338"
     CDS             complement(5576..7648)
                     /locus_tag="KPH29_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG1468905.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLSHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMVDNSMYASIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKLLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENALVEKIDSLTNGEILDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSKKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNCLSTISPVVFTNLEQNQKLSIKKGITTAKEENRVSIL
                     RYDIEKIG"
BASE COUNT         2669 a         1294 c          822 g         3048 t
ORIGIN      
        1 ttttgatata ataaaattac ttacatatat aacatttaaa tttataaatt atctttttat
       61 tttttaaaat atgtataaca cacctataga aattactatt attactatct aaagtatttt
      121 gctataatcc catatactct tacctcaaaa acattcaaat ggaaaaaata ctacatatta
      181 ataaactttg cccaataatt cctaaaataa tatcctaaca atgatttatg tttttataat
      241 agtatatttt tacataaaaa agtgaacatt gattaacaaa gttcactttt ttataaattt
      301 ttatgttata attcacaatt ttatttaatt tgtttctttt ctatttaaac tatatctatc
      361 taatattaat ttttcatatt cttctattgg cattggtcta gcaaataaaa atccttgggc
      421 catatcacaa cctataaacc ttaaaaactc tgcttgctct acagtttcaa caccttctga
      481 aacaactttt atatcaagtt gctttgccat ttgaacaata cttgaaacta caatttgact
      541 cttttttgca tttcccactg ttataaagaa ttgcctatct aatttcaata catcaattgg
      601 catatcttta agcatattta aagaagaata accagaacca aaatcatcca tagaaatttt
      661 aaatccaaca gatttcaaag tttgcattat tttaaataat atttttgtat tgtcaaatat
      721 tgcactttct gtcaattcaa tctcaattaa atttggttca atttcatatt cttttgttat
      781 tctttcaagc tcagaaataa aacctggatt ctcaagatgt actcttgata agtttattga
      841 tataggaaat gtggggaaat tttcattttc ccaacgttta aaatttttac aaacttgggc
      901 aaatacaaac atatccaatt ctgttataaa accatttttc tcaaaaatag gaataaatac
      961 acctggactt attagacctt ttttgggatg ttgccaacgt acaagcgctt cagcacctac
     1021 aattttagta gttgataaat caaccttagg ctgtaaataa actttaaatt gttcattttt
     1081 taaagcatca tgcatttctt gttcaatatc acgttcttct atgagagttt cttttaaatt
     1141 ttcagcataa atctcataaa cttttaaata ttttcttttt actgtagttt ttgcaatcat
     1201 agctttgtca agacaggtag aaatatttgt ttctccctct tcaacaaaat atacacctat
     1261 ggatggaatt aaattgagtt ctactcccaa acttgttttt atcattgaca attttttacg
     1321 tataagttca catatttcta taattgactc tctattattt ttcttttcaa atattatagc
     1381 aaaattgtca tttgaaattc ttgaaaatac agattgctct ttaaatatat tcttaattat
     1441 ctctgctatt tcctttaata ctctatctcc ttcttcatac ccaaacctat catttactag
     1501 cttaaactta tctatatcaa aataaattaa aactagattc tttttctcat gagaggataa
     1561 aaattttcct ccttcaagat taaatttatt tgaattgcca atacctgtaa cgttatcttt
     1621 aaaagcaaat tcttccaatt tttccttact ctttttatgt ataaatagta tataaattgt
     1681 taatgccgta aagattataa tcaacactac acatgctgat aatgtaagtt ttataatagc
     1741 gtatgatttc tcagatatag cagttttagg aacttcagaa aaaagatacc aatcattgat
     1801 tcctattggg gcataactta aataatatcc ttttccaaac atactataac ttaatgaacc
     1861 tgatttaaag ttttttatat ttgacttcat attattgata ctagattttg atattgaatc
     1921 acttctgcca atgtaagaaa ataaattttc tttagtagag ttggcgtttt tgctgcttgg
     1981 atttaaaaca atattaccat tacttctagc tatataagaa tatccttttc cattaaatgt
     2041 aggtacagaa agtatttttt catacaactt cgtactatgt gtggcaaata taactccttt
     2101 aacctcatta tctttataaa ttggcacaga atatacatta atattactat tggtattttt
     2161 atcatcttct attacatctt ttaatttttc tgttataacc acttctcctt gcattgattt
     2221 taagaaataa tctcttttag agaagtcttc tatataacca tctgtagtgt atgctgtacc
     2281 atttggtaaa ataactccca ttcttttaaa tgaactatta atagcttgtc tttttagtat
     2341 agaaaggata ttatctactt caaaagactc ttctccctca ataaatattg aaattgactc
     2401 tagtaacttt atatctccat taatttgttt ttttaacaca ttgacgcttt gatttgctat
     2461 ttctgaaaga tattgattcg tttcttcacc taatgtctta ttcaaccttg aaccataaat
     2521 caaacaggaa attgtaatta ctaagatact tatgacagca attatggaca aaccatacct
     2581 attatttttt gcatcactta acattatgct tcctgccttt ctcgaatcta tgtatgtata
     2641 atcattatct tatgacaatt attgtaaaca ttttataata atacttattt ttccatattt
     2701 actgtaatta tacaaaatta taaactttat tacaacaata aattaattaa tatagatatt
     2761 agggtttata ttattgcatt atctatttta caatcattta catataaaaa aatgtaatat
     2821 caaagtgaaa cttgatatta catttttgtt cttgtatttt gtttaatttt tatgctacat
     2881 tttacttaaa cactcatttg caacttcttt atcatcaaaa tgatgcttaa ctttacctat
     2941 aatttgataa gtttcatgtc ccttacctgt tataattaca acatcatcct tttttgccat
     3001 acttatagct tctgctatag cttgttctct atcaacaaca acatggtaat tttctttttt
     3061 cttgtcaatt ccttcaagta tatcttttat tattgcctct ggttcttcag ttcttggatt
     3121 atcagaagtt ataatacata aatcagaata tttttgtcca attgctccca tcaaaggtct
     3181 cttttcagta tctctatctc caccacatcc aaacacagaa attatttttc cttctgcaaa
     3241 ttgttgagtt gtctttagta cattttcaag tgcatcagga gtatgagcat agtctacaat
     3301 tacacttata cctttatcat ttggaactgt ttcaaacctt cctgatactc cattagaaag
     3361 tctaagacct tctttatata ttggtttagg tatgccaagt acatagcaag cagctataac
     3421 tgctaaagta ttatatactg taaacattcc tggaactggt ataaatatta cttcttcata
     3481 tgaaggtgtt attaatctat aagatactcc atccgaatct gattttatat ctctagccat
     3541 aaaatcagcc ttagtatcaa caccataagt gtaacaaggt acatttatac cttttatatt
     3601 ttcatatatc ttttttccac cttcatcatc tatgtttatt atatttgcca tagtagtctt
     3661 aaaaaataat ttttcttttg cttttctata atcctctaaa tctttatgaa aatctaaatg
     3721 gtctggtgtt aaatttgtaa atatgcctag tttaaagtct gtttcatcaa ctctatttaa
     3781 tgccaatgaa tgtgatgaaa cttccatcgc acagtagtca catccattat ccaacatatt
     3841 attaaaatga tattgtaaat ctatactttc tggagttgtt gaattggaaa caatgtcttt
     3901 ttctccgtca aaaattttta tagttcctat aagtccaact ttattcttat ttaaagttaa
     3961 tatctcattt agtatagtag ttatactagt ttttccatta gtacctgtaa ctcctataac
     4021 gttaaatttc tgagatggat gattataaaa attatcagct acttttgcca tatcttttct
     4081 tgtatctttt acctttataa atgtacatcc ttttatttct acatcttctt caacaagaaa
     4141 tgcacttgct cccttttcta tggcatcttt tatatactta tgaccatctg acacaaatcc
     4201 ttttatacat ataaataatg taccttttgt aacctttctt gaattatatt gtacattatt
     4261 aatatctata tttaattcac ccttgacatt gattatatcc aacccctgta taatatcatt
     4321 taatttcata aatgctttcc tccctttaaa ctctttctgc taaaattaca ctacatataa
     4381 tattatatta agaatttttt actatgtgaa tggttaaaaa tgcttgaaat attgcaaaaa
     4441 atgctctata attttctata atataactga ggaaaggtct tctataaaaa ctatggataa
     4501 taaaatcggg tacttaaacg ataactttaa aatatttaat attagagata aaaaagatat
     4561 acaatttgaa taccataatc atgattttaa taagataatt atatttataa atggaaatgt
     4621 aacttatttt gttgatggta ttccctataa actaaagcct tgggatatac tctttatcag
     4681 caataatgaa atccataaac cagttataga ttcaaatgtc ttttatgaga gaattgcaat
     4741 atggattagc ccagaattta tgaaaaaaaa tagtgatgct actagtgact tagaaaaatg
     4801 ttttaaaata gctacggaga ataaatgcaa tagattgcgt ttaaatatac atgatattga
     4861 atctattaag agttttattg agcaaataaa tatttccgaa tccaatactg aatttggaaa
     4921 tgaaatattg agaaatgcat tatttataca acttatgata ttcttaaaca gaattttcct
     4981 aaaaaatgaa aatatggaat caacagaaga cgttttttat gatgaaacta ttaagaaagt
     5041 attaaactat ataaattcaa atatagatga taagctttca atagatagca tatcgtctga
     5101 attttttata agtaagtatt atcttatgag aaaatttaaa tctcatactg gaacgtctat
     5161 acacaattat atagttcaaa aaagattaat tttagcaaag tcactaataa ccaatggtta
     5221 ccttatgagt gaagtttgta ataaatgtgg attcaacgat tattctagtt ttgtaagggc
     5281 atttaaaaaa aactacggtg tatctcctaa aaattatata aacaataaaa atcctttaga
     5341 tattactctt ttagatgaat agataattta tgtaaatttt attttaataa aaacttgaag
     5401 tattagtgta tttatcttac taatacctca agttttttaa tgttttaaaa tatatcaaaa
     5461 tataatgata tatatttcta ttacttaaaa tatatattaa ataaattgca tgttaactga
     5521 acctatttat atatttatta aatctcacat aacttttagt tatactatca tatagctatc
     5581 ctattttttc aatatcatat cttaatatac ttactctgtt ctcctctttt gctgtagtta
     5641 ttcctttttt aatgcttaat ttctgatttt gttctaaatt cgtaaatact acaggactta
     5701 ttgttgatag acaatttttt tcaatatatt ctaagtccat tttaactaat ttttctcctg
     5761 cttttacttc atctcctaca ttcacaaata cttcaaatcc atttccttct aaatttacag
     5821 tttctatccc taaatgtata agtatttcta ctccactttt tttggatttt attattattg
     5881 catgcttagt tggaaatata actccaatag ttccatcaac tggtgaataa atcaatccat
     5941 cactagattt aattgcaaat ccatccccca tcagtttact tgaaaataca gaatctggca
     6001 cttcttctat atctaatatc tctccatttg ttaatgaatc aatcttttct actaaagcat
     6061 tttccatatt ggcaacttcc atattttttt tcactttatt tttatcaaca tttttagtaa
     6121 cagtactttc aacctttgtt tttccctcta ttatatcttt tatatcagac tttattgaat
     6181 ccgattttgg tccaaatata gcttgaacat tgttgccaat aaccataaca cctgctgctc
     6241 caagtaattt caatttttct ttatctactt tatttatatc atttacaacc aatctaagtc
     6301 ttgttataca cgcatctagg ctctttaagt tgtctttatt tcctaaagca attaaaatct
     6361 ctgatgctaa ttcattctta gacatttcaa tgacttcagc gttagtttct gactcatctt
     6421 ctcttcctgg tgtctttaaa ttaaattttc ttatcataaa tctaaataca ctatagtata
     6481 atactgcaaa accaatacct accaagatag ccaagtacca tggagttcta ttaggaagaa
     6541 caccaaataa taagaaatct attaatcctc ctgaaaatgt gaatccaatt ttaactccta
     6601 ataattgcat aattgcgtat gataaacctg aaaataaaca atgtattgca aataatactg
     6661 gtgcaacaaa taaaaacgca aactctattg gctcggttat tcctgttaaa aaagaggtta
     6721 atgctgctga tgccattata cctgctacaa attttttctt ttctggcttt gcttcatgca
     6781 ccatagcaag agctgctgct ggaagaccaa acatcataaa tggaaatgca cctgtcataa
     6841 aagtacctgc tgtgaaattt actccatctt taagttgagc aaagaatatc ttttggtcac
     6901 ccattacaag ctcaccagct ttatttacat attctccaaa ttgataccaa aatggattat
     6961 ggaaaacatg atgcaaacca aatggtatta atagtctttc caaagaacct tccataaatg
     7021 ctgctagtgt ttgatttgct tctataacat tttttgagaa actaagtaat ccctcttgta
     7081 ttggaggcca aataaaagac aatataattc ctattgctat agcaactaat gaagtaacga
     7141 ttggtacaaa tcttttacct gcaaaaaaac ctagatactg aggtaattca attttataaa
     7201 actttttata aagataagca gccactatcc ctataataat accaccaaat acccctgttt
     7261 gaattgttgg tattccaaca atacttgcat acatagaatt gtctaccata tcttctgtaa
     7321 cacccgccat aacacccata gatgtattga gaattaaaaa acctactatg gctgccaatg
     7381 ctgcaacacc ttctccttca gttaaaccaa cagctacccc aactgcaaat ataagtggta
     7441 agttattaaa tataatttga cctgaacttt ccattatact tattataagt tgaaatgtag
     7501 gattcgccaa agctggaaaa tgagacaaca atgcgtctcc tgctaaagca ttactcacgc
     7561 caagtaatat acctgctgca ggaagtaatg caactggtag cattagagat tttcctactt
     7621 tttgtaaaac accaaatact tttttcattt acttttctct ccttattatt ttatattttt
     7681 ttaaataaaa aagacttaag tttataaaaa gttacattag tatacttaat tctaaaatta
     7741 aacttatgtt aatataaata aacctatatt aacctaattt cagaattaaa aataaactat
     7801 tataattttt ttataaacat aagtcttgcc tgc
//
LOCUS       DAELXX010000149         7509 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.151, whole genome shotgun sequence.
ACCESSION   DAELXX010000149 DAELXX010000000
VERSION     DAELXX010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7509
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.151"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            129..1106
                     /locus_tag="KPH29_003339"
     CDS             129..1106
                     /locus_tag="KPH29_003339"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBG1468906.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLDTIDSTNEHAKRIAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKTHEGIWMSIILKPDMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            1099..2067
                     /locus_tag="KPH29_003340"
     CDS             1099..2067
                     /locus_tag="KPH29_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HBG1468907.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLIVSALIAVNALGDVYENGKVIAGALNDKKTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            2251..2841
                     /locus_tag="KPH29_003341"
     CDS             2251..2841
                     /locus_tag="KPH29_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG1468908.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTVTSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            2860..3630
                     /locus_tag="KPH29_003342"
     CDS             2860..3630
                     /locus_tag="KPH29_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HBG1468909.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            3767..4735
                     /gene="dusB"
                     /locus_tag="KPH29_003343"
     CDS             3767..4735
                     /gene="dusB"
                     /locus_tag="KPH29_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBG1468910.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRAAEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            4844..5323
                     /gene="greA"
                     /locus_tag="KPH29_003344"
     CDS             4844..5323
                     /gene="greA"
                     /locus_tag="KPH29_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBG1468911.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            5338..6867
                     /gene="lysS"
                     /locus_tag="KPH29_003345"
     CDS             5338..6867
                     /gene="lysS"
                     /locus_tag="KPH29_003345"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBG1468912.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            7176..7370
                     /locus_tag="KPH29_003346"
     CDS             7176..7370
                     /locus_tag="KPH29_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468913.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NIWADRVRANSYSYENKIAK"
BASE COUNT         3003 a          756 c         1512 g         2238 t
ORIGIN      
        1 tataaaacaa ccattataat aattaaaaag gaaatttaaa attaatagta ttaaaagtat
       61 attaataatg tataattatt tattagaaga aaataagtca tgaaattatg tcatattaga
      121 ggtgaaatat gcgagataaa ataatagaga ttatattaaa taataaatct gaatttattt
      181 caggagaaga attatcaaaa caattagggg tgtcacgtgc agctatatgg aaacatatga
      241 aagccttaaa agaagaggga tataatatag aatctgtaaa taaaaaaggg tatcgattag
      301 cggaaaatcc aactgattta ttaagcccac agaatattta ctataaatta gatacagagt
      361 ttattggaaa aaatattgaa catcttgaca ctatagattc aacaaatgaa catgctaaaa
      421 ggatagctat aaatagtgta gatgggactg tgattttaag tgaacatcaa gatatgggga
      481 aaggacgatt gggaagaaga tgggattcaa aaactcatga aggaatctgg atgagtatta
      541 tattaaaacc agatatggaa ccattcaaag ctccatttat aactttaata gcaggagcaa
      601 gtgtaacgaa agctttaaac aatctaggag caaatacatt aataaaatgg ccaaatgatg
      661 ttattttaaa tggaaaaaaa atatgtggta tattaacaga gttatctgct gaaattgaaa
      721 gaacaaatta tgttgttctt ggaattggaa taaatgttaa aataatggat tttgaacaag
      781 aaatagctga caaagccaca tcattgtata aagaagggta tatgttatcc agagtagata
      841 ttgttaggaa tgtattgact gaatttgaaa aattatattt agattatact gtaaataatt
      901 ccaaagaaaa aaccttaaaa atatgtagag attattctgc aattatagat aaaaatatat
      961 atgtattaaa aggagataat agagaactag tgaaatgttt agacataaat gaagagggaa
     1021 atttattagt tcaaagaagt gatggaagta taaaagaaat tatttcaggt gaagtttcta
     1081 taagaggaga aaaaggatat gtataataat attttggata tagaaggctt aaaggttggg
     1141 caggttgagg atagaaatgg tcttactggg tgcacagtag taatatgtga agaaggagct
     1201 acttgcggtg ttgatgtaag gggttctgct ccagggacta gagaaaccga tttattagac
     1261 cctgtaaata tggttcaaaa agttcatgca gtagttttgg ctggaggttc tgcatatggt
     1321 cttgaatcca cttgtggtgt aagtagatac ttagaagaaa aaggaattgg ctttgatgta
     1381 ggtgtaggta aagtgcctat agttgtagga gcagttttat ttgatttagg agtagggagc
     1441 tttaaatgta gaccagattt aaagatgggt tatagagctt gtgaagttgc aaatactgat
     1501 attttaaatc aaggaaatta tggagcaggt tgtggagcca ctgttggaaa aataagaggt
     1561 caggaatatg ctatgaaagg cggaataggt agctattcta taaaattaga taatggtcta
     1621 attgtatctg cattaattgc tgtaaatgct cttggagatg tttatgaaaa tggtaaggtt
     1681 atagctggtg ctttaaatga taaaaagaca gaaacaattg atacatataa attaatgaaa
     1741 caaggtgaaa gtaaaggtgg atttagtgta gataatacta caattgggat aattgcaaca
     1801 aatgctaagt taaccaaagc tggatgtaaa aagatatctc aaatgggaca tgatggatat
     1861 gctaagtcta tttttcctat ccatacacct catgatggag atacaatatt cacaatatca
     1921 acaggagaaa tagaagcaga tataacatta gtaggttcat tggctgtaga ggttgtggaa
     1981 aaaagtataa taaatgcagt aaaaaaagcg gatagtgtag aaaatataat ggcatataaa
     2041 gatatagtaa aaaaacgaga aatatgatga aattagcata tttttcttgt caaattgttg
     2101 acaatggcaa ctcatcttga taaaattatt cttgttagag cgggcatcct acaatggtag
     2161 cttgactcat agttataaga aaaaaacgaa aaaaattaaa aaattaagta gtccggcatc
     2221 ttaatgagct ggaacggagg tgtatttttt atgatgaaca caagtgcatc agtaagaaaa
     2281 gtaaatgtaa gaaagatgac tataatagga gtactatcag ctatttctat tatgctatct
     2341 atgactccat tgggatttat tccagttgga cctacaaaag caactataat gcacatacca
     2401 gttattattg gagctattat ggaaggacca atagttgggg cagcaatagg atttatattt
     2461 gggatatcaa gtctcttaaa tgctataaca aacccaacag taacatcttt tgtttttata
     2521 aatccattag tttcaatatt accaagagtt atgataggtg tattagctta ttatgtatat
     2581 cagttaataa ttaaggctac aaataaggta tacatatcag gtttaattac aggggcaata
     2641 ggttctctgt taaatacagc tggagtttta ggaatgatat atgtcttata tgctgacaag
     2701 tatcttcatg caatgggtaa aagtggttca gcaggaaaag taataatggc tcttgccgca
     2761 gcaaatgggg tgccagaagc aatagtaggg gctttagtag tagctgctgt tgccactgta
     2821 cttaagaaaa gcaaaaaata gaaagaaggt taatgacata tgcttctagt atttgatgtt
     2881 ggaaatacta atatggtttt aggtatatat aaaggtgaca aattagttaa ttactggaga
     2941 attaaaacag atagggaaaa aacgtctgat gaatatggaa tcctgataag taacctattt
     3001 gattatgata atgtgaatat aagtgatatt gatgatgtta taatatcatc tgtagttcca
     3061 aatgtcatgc attctcttga aaacttttgt ataaagtact gtaaaaaaca gccattaata
     3121 gtaggtccag gcataaaaac aggtctaaat ataaaatatg ataatccaaa acaagttggg
     3181 gcagatagaa tagttaatgc tgtagcaggg atagaaaagt atggagcacc aagtatactt
     3241 gttgattttg gaacagcaac tacattttgt gctatctctg aaaaaggtga atatttgggt
     3301 ggaacaatag caccaggaat aaaaatatct agtgaggcgt tatttcaaag tgcgtctaaa
     3361 ttacctagag tagaattagc taagccaggt atgactattt gtaagagtac tgtatcagcc
     3421 atgcaatctg gaataattta tggatatgtt ggtttagttg acaaaataat aagtatgatg
     3481 aagaaagaat tgaattgtga tgatgttaag gttatagcta caggtggatt agctaaactg
     3541 attgcttcag agacgaaaag tatagattat gtagatggtt ttttaacact agaaggattg
     3601 agaataatat atgaaaaaaa ccaagaataa ttaatataca gggaattggg ttatatatcc
     3661 aattcccttt ttatgataga tttctagcta tatgtggtat attataatga ggaaataaga
     3721 atttaaagtt aaatcacaaa ataaaggcaa aaataggaga aaaacaatga aaataggaaa
     3781 tttagaattg aaaaataaag tttttctatc acctatggca ggtgtaacag accttccatt
     3841 tagattaatt tgtaaagaac agggatgtgg gcttttatat acagaaatga taaatggtaa
     3901 ggcactttgc tatgatgatg aaaatactaa aaaaatgctt aagatagaag aggaagaaca
     3961 tcctgtagca gttcagatat tcggttcaga accagagttt atgggaagag cagcagagat
     4021 aatgaatgat tattcaaatg agattctcga tataaatatg gggtgtccag ctccaaaagt
     4081 agttaaaaat ggagatggtt cagcacttat gaaaaatcct aagcttgcag aagaggtatt
     4141 gagggcagta gtgaagaact caaaaaagcc agtgacttta aagattcgaa aaggctggga
     4201 tgataatagt gtaaatgctg ttgaaatagc aaagatagca gaggattgtg gaataagcgc
     4261 tttagcaata catggaagaa ctagagaaca gttttataca ggcaaagcag actgggatat
     4321 aattgcagag attaagaaaa atttgagtat acctgttata ggaaatggtg atgtatttac
     4381 aatagaagat tcaataaata tgttagacaa aactggttgt gatgctataa tgataggtag
     4441 gggtgctcaa ggtaaccctt ggatatttaa aagaataaac cattatatga atacaggcga
     4501 gattttgcca gagccaactc ttaacgaaaa aataagtact gccattaagc atttaaaact
     4561 tgcagtagaa gaacatggag agtatgtagc tgtaagggaa atgagaaaac acatcgcttg
     4621 gtatttaaaa ggacttagaa actctgcaaa attaagggat gaaattaaca aaatagaaga
     4681 ctatcaagaa gttgtgtcga aattggagta ctatatggaa gactccttga cataaaaata
     4741 atagtgactt ataatatagc gcataaatta aaaaaagatt ataaaatttt taaaatataa
     4801 ttaggaaaat tcctaagaaa tttgtgaagg agttgtattt ataatggaag aaaataaaga
     4861 gtttttattg acacaagagg gatatgataa attagaagaa gaattagaaa acttaaaggt
     4921 tgtaaagaga aaagaagttg cagagagaat aaaagttgct atatcttttg gtgatttatc
     4981 tgaaaatgct gagtatgatg aagctaaaaa ggaacaagca caagttgaag aaagaatttt
     5041 aaaattagaa aatatggtta gaaaagctgt tatcatagat gaaagcaaaa tagaccttaa
     5101 tgttgttaca ataggatcaa tagttaaagt taaggattta gaatttgatg aagaggttga
     5161 atatactata gttggttcga cagaagcaga cccatatgat ggtaaaatat caaacgaatc
     5221 tcctgtaggg aaagcacttt taggtagagc tgcgaaagaa gttgtggaag tgcaagttcc
     5281 tgatggagtt gctaagtttg aaatattaga aataagaaga taatttggag gaaaaaaatg
     5341 aaaaataatc aacaaagcaa tgaagaagcg caaattcaag aagaccttag tgaagtactt
     5401 caagtaagaa gagataaact aaaaaaatta caagagtcag gtagagatcc ttttaaagaa
     5461 agtagatatg atagaactca ttattctatg gatataaaag ataactttga ttctcttgaa
     5521 ggtaaaacta caaaaatagc aggacgtata atgagcaaga gaatacaagg gaaagctggc
     5581 tttatagata ttcaagacca agagggaaga atccaatctt atgtaagact agatgctata
     5641 ggagaggaag aatatagtgt attttcaaca tatgatattg gagatatagt tggaattgaa
     5701 ggagaaatct ttaaaactaa aaaaggtgaa atatctgtaa aggctaaatc tgtagtatta
     5761 ctatgtaaat ctttacaagt tttaccagaa aaatatcatg gattaaaaga ccaagaacta
     5821 agatatagac aaagatatgt tgacttaata gtaaatccag aagtaaaaaa tgctttttta
     5881 attagaacaa aagcattaaa agcattaaga gcatacttag atgatagagg atttttagaa
     5941 gttgagactc caatattaaa tactatagca ggtggggcaa atgctagacc atttataact
     6001 aatcataata ctttacatat accaatgtat ttaagaatag caaatgaatt gtatttaaaa
     6061 agacttattg ttggtggatt tgataaagtt tatgaaatgg gaagaatgtt tagaaatgaa
     6121 ggtatggatt taaaacataa tcctgaatat actgcaatag aattatatca agcatatgca
     6181 gactatactg atatgatgga aataacagaa aatgttattg cacatatggc tgaagttgct
     6241 acaggaagta tgatagttaa ctatcaagga acagaaataa actttacacc accttggaaa
     6301 agaatgagta tggaagattg tgttaaagaa tattctggtg tagatttttc tactataaat
     6361 acagatgaag aggcactaga agttgctaga gaaaaaggta tagagataaa acctggaatg
     6421 agaagaggag aagtaataaa tgctttcttt gaagaatttg gagaagataa attaatccaa
     6481 ccaacattta taactcatca tccagttgaa gtatctccat tatctaagag aaatgttgaa
     6541 gacccaagaa gaactgatag atttgaggca tttgctaata agtgggagtt agctaatgca
     6601 ttttctgaac ttaatgaccc tattgaccaa aaaggaagat ttatagacca attgagaaaa
     6661 agagaattag gtgatgatga agcttttgaa atggatgaag atttcttaaa agcattagag
     6721 gtaggtctac ctcctacagg aggactagga ataggtatag atagagtaat tatgttatta
     6781 actaactctc catctataag agatgtatta ttattcccga ctatgaaacc tattgataac
     6841 aatccaaata aagaagaaga aaactaagaa atattagcat tctactaagt tttaaataaa
     6901 gtttagctat taaattagca ataaatagct aaattttgtt taaaatgtca gtgtagaatg
     6961 ctttttatat gcttcaaaga ttcttttaga tatttaattt ataaattacc aacaaacgtt
     7021 agaaattgat attagttttt gttgattgag actttatatt tagaaaatat gattgaaagt
     7081 aatttattat gaaaatattt agaataattt atgagaaggt aaaaagaata atttatgaga
     7141 atactatata taaaaaatta ttgggggaat gtctgatgtt tcaagtaatt aattttttat
     7201 gtttattttg tgttggattt ttggttcttg atgttatgat atatttttgt aaggatgcaa
     7261 tggatatttt tgagggtaca actaatgtta ataaagttaa gagtataaat atttgggcag
     7321 atagagtgag agctaattca tattcttatg aaaataaaat tgcaaaatag gtaaaattat
     7381 atatgcatgt attgtttgta atatcaaggg ttatatctat tagttttaat tatatttatt
     7441 aaaaaaacca aaagttataa ccttttttaa aataaaaaac aaaaaaagta ttgacgaaat
     7501 atttttaag
//
LOCUS       DAELXX010000150         7493 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.152, whole genome shotgun sequence.
ACCESSION   DAELXX010000150 DAELXX010000000
VERSION     DAELXX010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7493
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.152"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            88..981
                     /locus_tag="KPH29_003347"
     CDS             88..981
                     /locus_tag="KPH29_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG1468914.1"
                     /translation="MQLYNTPFNTNKHLIYFFTSTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEIRIKWILLTILFFALTFIT
                     SLIIGNYFNVKTSDIDWIHIAVFRPSKIAYMLLASFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGLVCGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMMYYFFNIQLLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDW"
     gene            1159..1983
                     /locus_tag="KPH29_003348"
     CDS             1159..1983
                     /locus_tag="KPH29_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG1468915.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAEIDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEIKHEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREVVPDYQSEGILWEKLSKFIKEEHIE
                     VSRQPNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            2082..2690
                     /locus_tag="KPH29_003349"
     CDS             2082..2690
                     /locus_tag="KPH29_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG1468916.1"
                     /translation="MEKLLRGKIMKADSTKEQLINATIQLLSSHRDVSSITAREIVAK
                     ANVNLAMINYYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFD
                     FIGEHSQFIKISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSF
                     IQLLFLRSNAFFKYSGIDILNVEQRNGLIDFQLDLLLVDITK"
     gene            2794..2970
                     /locus_tag="KPH29_003350"
     CDS             2794..2970
                     /locus_tag="KPH29_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468917.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
     gene            4266..5018
                     /locus_tag="KPH29_003351"
     CDS             4266..5018
                     /locus_tag="KPH29_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1468918.1"
                     /translation="MLKVNQLCKSYKTGNKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            5008..7119
                     /locus_tag="KPH29_003352"
     CDS             5008..7119
                     /locus_tag="KPH29_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1468919.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCAVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISIVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYI
                     VPSLTINKLNYRLPSVWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVAGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIERVYMKKSSYDMFFNSK
                     DFSKLIDKPITIKNGEYLAVVNKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNDEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIGIL"
BASE COUNT         2775 a          971 c          988 g         2759 t
ORIGIN      
        1 atcaaacgat aacttaatac atataaaaat aaataaacaa ttaataaaaa ttaaaacttc
       61 attataaaac ggaaggatag tgaaaaaatg caactatata acaccccctt taatacaaac
      121 aaacacttaa tctatttctt tacatctacg ctagtattga actggatatt cagtcttaca
      181 cctgcgttgc tcggaattac aaatacaata cttggagaaa tcatttttta tcttgcatat
      241 acatcaccag ctattgtagg tctatttttt atttctacaa tctatccaca aagtgcaaaa
      301 caagatttta tttatagata cctaagcttt aaagaaatta gaataaaatg gatactatta
      361 acaatattat tttttgcttt aacttttatt acttcgttaa taatcggaaa ttactttaat
      421 gtaaaaactt ctgatataga ttggatacac atagcagtgt ttagaccttc taaaatagct
      481 tatatgctat tagcaagttt tatattgagt atacttagtc aagaagctgg atggagagga
      541 tacgccattg acaaattatt agttagattt ggatttatag gctcttcaat tattttaggg
      601 cttgtttgtg gaatctggta tctagggtcg tattttacac ctaatcaaat accatataat
      661 ttggcacaat actccctatt tgatgctttt ttatttatac caaacattgt acttttaaac
      721 tttataatta attttgtata tatcaataca agtagaagca ttttatcagc tgggttagtt
      781 catatgatgt actatttttt caacattcaa ttacttctac actaccctat taaattatgt
      841 gtaatagctc aatatgtaca aataatattt ggtttaatat ttcttgtata tatgatttct
      901 tctaataaat ttaaacagaa ggtaaattct gaaattgaac agataaagtc ggatgatttt
      961 gaatttgagt tagactggta attttaatat gaattataaa ttaagataat ctatgatttg
     1021 atacgattac tacttaaata taatttactc tctataattt attgcaacat ctggaaaata
     1081 attgtatttc cacaatcgct tgaagtatat aaaaacaaaa tatatactaa ttataagtga
     1141 aaaggaggtc atattttcat gtttaaaata ggagaggttt caaaactaac acaaatttct
     1201 attcgtatgt tgcgttatta tgatgaactt ggtattttaa agcctgcaga aatagacaaa
     1261 tatacaggtc atagattata ctcagttgaa cagatttcaa tattacaaag aattgtatta
     1321 ttgagagatt caaaatttag cgttgcagaa atagcaaata tcgtacataa ttggaatgat
     1381 gaatttgtaa ttaaagaatt gaatagaaaa aagaatgaaa tacaaaagga aataaagcac
     1441 gaacaacaaa gaataaataa aattgataaa tttatagaag ctataaactg tgataaagat
     1501 gaaattcatt acaatgttgt ctttaaaaag attccaagtt ataaaatcat atcacttaga
     1561 gaggtagtcc cagactacca atctgaaggt atactctggg aaaagttatc taaatttatt
     1621 aaagaagaac atatagaagt atcacgtcaa cccaacaata atatagcttt ttaccatgat
     1681 gaagaagtca aagataatgg tgtagatatt gaagttggaa tggttgtaaa gaagattgga
     1741 aagaacaaat ctggatttat atatcgtgag acagaagaaa ttgatatgat ggcatgtact
     1801 atggtttatg gtccatatga aaatatagct ggtgcatatg aatcgttctg ttattggtta
     1861 gataaaaata gtgattatca aatatcaggt ataaatagac aaatcggtca taagggtgag
     1921 cataatgaaa taaatcctga aaattatctt acagaaattc aaatccccct tataaaagtc
     1981 taatcgatta gtacttaaat acttaagtac taatcaccca ttgacatccc aaaattttca
     2041 tgatagtatc taaacgtgta ttaaacatgt ttaatttttt tatggagaaa cttttaaggg
     2101 gaaaaattat gaaagcagat tcaacaaagg aacaattaat taatgccaca atccagttac
     2161 tatcttcaca tagagatgtt tcaagcatta cggctagaga aattgtagct aaagcaaatg
     2221 tcaatttagc tatgattaat tattatttta aatctaaaga ggagttaatt aatatttcta
     2281 ttaaaaaatt tttaaagcgt catatggatg aatataaggc tacacatgat actaaaaatt
     2341 tagcacctaa acagcaatta agggaaatac taattgcatt ttttgatttt ataggtgaac
     2401 atagccaatt tataaagatt tctgtaccct atatattgat acaagaagaa attgtttatc
     2461 catttgagat actaccttta attaaggctc actatgaaaa tcagaataac aaaaaaactg
     2521 aagctgaata cagaactatt gcgtatcaaa taacttcatt tattcagttg ctttttcttc
     2581 gttctaatgc ttttttcaag tatagtggta ttgatattct taatgttgaa cagagaaatg
     2641 gtttaataga ttttcaatta gatttattat tggtagatat tacaaaataa tactatatta
     2701 attttttatg tacatttttt tattttatgg taacgtttga aaatattctc attgactaaa
     2761 aatacttgca ttaaataagg gggaggaatt ttaatgtttt ttacatttat aggagtaata
     2821 tcagcaattt tattgttgtg tacaattata tgtggtttat ggataaaact taataatcgt
     2881 aataatgact ctaattctca ctttactttt tcaatacttt caattgcttt ttcactgtta
     2941 attgtggtgg cctttttagt aagaaattaa tgtctttgtt atgctggggt ttattattat
     3001 agatttttat tattgcatga tttttactat attatagtat ttttattata ttacagggtc
     3061 ttatttatta tagtgttttg ttattacagc attttattat attacagagt cctatttatt
     3121 ataggatttt tattatattg taaaatctta tttattatag gattttgtta tattacagac
     3181 ttttattata ttacaatctc ttatttattg cagtgttttg ttattacagt atttctatta
     3241 tattgcaaac tcttatttat tatggtgttt tgttattgca acatttctat tatattataa
     3301 agtcttattt attacagttc tttgttatac tacagacttt tattattaca gcatttctat
     3361 tacaatctct tatttattac agttctttgc tatactacag acctttatta ttgcaacatt
     3421 tctattacat tgcaaagtct tatttattac agttctttat tatactacag acttttatta
     3481 ttacaacatt tctattatat tacaatctct tatttattac agttctttat tatactacag
     3541 acttttatta ttacagcatt tctattacat tgcaaagtct tatttattac agttctttat
     3601 tatactacaa acctttatta ttacaacatt ttattatttt aacgtcttta ctataatata
     3661 gtaatatttt aatatctcaa aatcatatca atcacttttc agcatagtct aaaatacttc
     3721 cactttctaa aattatctat catacctata ctaataatac tttttaacct ttttattata
     3781 catttcaatt gtttttaacg acctatttat ttccaaaact ccaatatatt ttctcaatta
     3841 cattgtgtta ataaccccta aatcctttca aattatttac aatacattcc attaaataat
     3901 cttactgctc tataattctt tttttctttt taaccctact atacattatt tacatcattt
     3961 ttttcaatac aacattcgtt attctataac ctttttactt ttactatata ccttttttct
     4021 aactacacct tcaatgttcc acatcatatt actttagcac aacaattaat acctaaaaat
     4081 aaccctctaa atcctccaat aatataatca atttaactat aataataacc tctcacacta
     4141 tccaaacaat atattttcaa acattacaaa aaaacatcca actattacaa tattgctcat
     4201 atccctcctc ccctttcaaa tgctatcatt gacttacaag ataaatgaaa gtgaggatga
     4261 tatatatgct taaagtaaac caattatgta aatcatataa aacaggcaat aaaacatatc
     4321 ctgtattaaa aaatatttct tttgaaatca acaaaggaga gtttgtcgca atcatgggtt
     4381 catcaggaag tggaaaaact actcttttaa attgtatttc atgttttatc ccaattgata
     4441 atggggaaat aattcttgga aataataata taagaaattt aaaagaaaag gaactttcag
     4501 aagttagaaa ccaacaacta ggatttgtat tccaagattt catgctattt gatggtctta
     4561 gtgtctttga aaatatatgt gttccacaaa ttatcaaagg tagtcaagtc ttacaaatgg
     4621 aaaaccgtgc aattaatcta tgcgagctat ttggtattga acatatcaga aaaaaatacc
     4681 ctgccgaaat atctggtgga gaaaaacagc gtacagcagt agcaagagca ctcatgaaca
     4741 acccagattt aatattagca gatgaaccaa ctggtaacct tgatagtaag tcttgtcgta
     4801 cagtcattga atcattttta aaagcaaagc aagaattaga tgctactata ctcatggtta
     4861 cacacgatag ctttgcagca agcttctgcg accgagttat tgtgcttaaa gatggaataa
     4921 tttataaaga agttgtaaaa tcaaattcaa gacataaatt cctagatgaa ttactagaag
     4981 ttttaaaaga aatgggaggc gctcaagatg acaattaata aattatacag caaacttcga
     5041 gcctataatt ggaagaacta tacaacttta ttcttttgta taatactttc tgtggtatta
     5101 gtaacttcat atgctctaat ttattttagc ccaacaatac aaaacatact tccaactggt
     5161 ggagatagca gaaaacaggc tacaatgata ttctcagttg ctatattagg atgcgctgtc
     5221 tttactacat acgcttcttc cctcttttta aaatttaaaa gcaaggaaat tggtattttc
     5281 atggctctag gaacagataa gaagaaatta aaaaaacttc tattttcaga gatattgtta
     5341 atttcaatag tatgctgttt tttaggtctt atattaagta ttcctgtatc ttttggtata
     5401 tggaaaatat ttcaattagt gattattgat accagagaaa tgacatatca ttttggaact
     5461 caaggattca tatatggatt tatatttagt gtatttgtga cattatgcat atttatactt
     5521 tctgtaaaat ttattaatcg tacaaatata attgatatat taaatgacca gagaatatct
     5581 gaaccaatac aggatgtaaa ggactggtat ggtatattag gtataatttt tattatacta
     5641 ggtatttttc taggatatat cgtaccttca ctaactataa acaaattaaa ttatagacta
     5701 ccatctgtat ggagctttac ttatctactc tctcttattg gaatatatat gattatgaca
     5761 tacatagttg tacatgctaa aagaggaaaa catccagaaa aatactataa aaacatcata
     5821 tcaactagca tgatgagatt tatgggaaga caaacagtaa aaaacatgtg tgttatttca
     5881 tttttgattg caggagcatt atttgctgta ttctatgttc ctaatatggt tgcaggtatc
     5941 tatgatactc ttaaaaataa ccctatagat tatactttta cctacaatca aagagataaa
     6001 caggtatcaa aaaaagatat tgaacaatta gcagataaac acaatataaa aattcaaaac
     6061 tatatggaaa ttccttctat agaattaatt gttgatggtg tagaatcaat atatcataaa
     6121 gatggaacta tcgaaagggt atatatgaaa aaaagctcct atgatatgtt ctttaattct
     6181 aaagattttt ccaagctaat tgacaaacca ataactataa aaaatggtga atatcttgct
     6241 gttgtaaata aaggaaatga aaatcaagat tataaatata ccaaatttat atcaagccca
     6301 gtcacagaaa aagaaatccc ctttagatat gctggaaaaa taatatttaa tggttctttt
     6361 tttcaaaata gaagtatgaa cgcctatgtc cttaatgatg aggattataa atcatttact
     6421 aaagatttat ccataaaaaa ttacaataat tctatactat ttaatgtaga taaagatacc
     6481 tatgcttttg ccaatgactt aaagaaagaa attataaaaa gaacatctaa ggaaatgggc
     6541 atgttcagtg gttatgatga atacgtaaaa aaacgttatg aatcaactgg aagaaaatat
     6601 tttatggatg aagaatatcc aagtggtgaa ggtcatttaa aactagaccc aaacaacaat
     6661 cagttgtatc ttaattggaa atactatcct aactttaaaa ttttacaaag ccaagatatg
     6721 ataaaaaaca tggcagtctt cttaatgtta ttcatatatg tggcaatcat atgttttaca
     6781 gcagtaggaa tcattgcata tacaaggggt gttactatag ctataaatca caagcaggtc
     6841 tttatggatt taaatcgatt aggagcaaac aatgattata taaagttctg tattagaaat
     6901 caattaaaga aagtattttc ataccctctt attgcaggaa gttttttaat ttatctgttt
     6961 aattttatga ttatgttaaa taatgatggt agaatcgtca atagtgaggt caatgctctt
     7021 ttgataaact taggttttat agggatttgt ggagtttata tgtaccttat atatctatta
     7081 acttataaga aattcaaaaa aattattggt attttataat aaggaaggtt gttagatttt
     7141 taaagtaata aaatcattac tttttataat aagaaaagca ttagcatttt aaattaaaaa
     7201 aggttattag atttttatga tatgaaaagt cattgtattt ttatagtaag ataattattt
     7261 gctttttata atatgaaaat ccattatatt tttatagtaa gataattatt tgctttttat
     7321 aatatgaaaa gtcattgtat ttttatagta agataattat ttgcttttta tagtattaaa
     7381 agtcattgca tttttatagt aagataatta tttgcttttt atagtattaa aagtcattgt
     7441 atttttatag taagataatc atttgctttt tataatatga aaagtcatta cat
//
LOCUS       DAELXX010000151         7350 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.153, whole genome shotgun sequence.
ACCESSION   DAELXX010000151 DAELXX010000000
VERSION     DAELXX010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7350
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.153"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(127..654)
                     /locus_tag="KPH29_003353"
                     /pseudo
     CDS             complement(127..654)
                     /locus_tag="KPH29_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
     gene            938..1504
                     /locus_tag="KPH29_003354"
     CDS             938..1504
                     /locus_tag="KPH29_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG1468920.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRNRVNKND"
     gene            complement(1695..3731)
                     /locus_tag="KPH29_003355"
     CDS             complement(1695..3731)
                     /locus_tag="KPH29_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HBG1468921.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLKNVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDEKYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGTFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LKLYVDNVKSSPTGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIIGFLNNYRLKDDKTSYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(3905..4582)
                     /locus_tag="KPH29_003356"
     CDS             complement(3905..4582)
                     /locus_tag="KPH29_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG1468922.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNAVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSRLYFDLSTPGPNHNTKIDINPPS
                     FEKPLKK"
     gene            5277..5462
                     /locus_tag="KPH29_003357"
     CDS             5277..5462
                     /locus_tag="KPH29_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HBG1468923.1"
                     /translation="MILLEQRKLRIGFNKSGKGSFTPRLILPMSWIKEMNISPDERDV
                     LVTFEDGKIIIEKTENE"
     gene            complement(5503..>7350)
                     /locus_tag="KPH29_003358"
     CDS             complement(5503..>7350)
                     /locus_tag="KPH29_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG1468924.1"
                     /translation="LKNNKNLVSLFFTSNELTEIDLSNNVNLENLQFLNNNLKSIDLS
                     HNKKLKSLNCTNNKLKSLDLSNNVFLKNLSCSGNNLSKLNIKGLTSLEYLGCAKNKLQ
                     DLDVSTNIKLDSLLCGKNSLTKIDISKNKELNSLECSGQYIEKSIIKNGENIVVDIGI
                     ENSKLKELYPQNYNISTGFLTWENLSDFPNSISYSLEYEYGPVNDTETMDVNISIDKL
                     VKCRIRYIDYDGSIINEKEISDNSSINEIQINPQRENYEFSGWRNNKDEKIYTSEQVS
                     KLVFDDSTDFIATYKRKSTGSSGSSGGTSSKEDFNIAPSKEKITGADRNETSVKISQK
                     GWNKADNIVLINDSSISDALSATPFAKSKDAPILLTKNNNLNKLTEKEINRLEAKNVY
                     IVGGLKSVDEKVVSDLKKKGLNVIRISGNDRYETSIKLAKELDKNSNLSKVVVVNGEK
                     GLADAVSMGAISAKEEMPILLTNQNDDMKDIKDLIANKNISKSYVIGGESLFNNKEVN
                     NTLPSVTKIAGSDRTETNSKVISHFYSKDTLNDLYVAKNGMNKQDDLVDALSVGVLAG
                     KTESPVVLVGNGLDDSQKELIKNKKFKNITQIGGNGNEKAFNEVENLVK"
BASE COUNT         2424 a         1162 c          813 g         2951 t
ORIGIN      
        1 ggtttccagc aattcaagta gtattggata ggttctatcc tatctctaca tataggtttc
       61 cctatatgct gactatttta gctactaact catgactaaa gtcacaagtg tgcatagcta
      121 ttttttaatc aaattattta ttttattact tttagaacgt cttccatact ttttaatctc
      181 acgattgatt atcagatgag caagatttag aatgattcct tgctttccat ttgaaccaca
      241 tagtataaga ccagataaat tattactgta atccattata tatctttgac ttgcaaatga
      301 acccatgctg tgtccaaata gataaattgg caaatcttca ttttctttct ttataatatt
      361 tgttaaagta tacatgtcct ctacaagaca tctaaatcct tctttttcag ctaaatgtcc
      421 aacattttct attatctttg cagtttttcc atgacctcta tggtcattga tatatactat
      481 ataaccattt ttagtaagta ccttggcaaa agtctcatac ctttgtgcag tttcagccat
      541 tccatgtgca atttggataa ctgcctttgg tttttttata ttttcatctt cccatttata
      601 tgtatatata tctaaacctt cctctccttt gaaagtaaag tttgtacact tcataattat
      661 ccctccagat tgattttatt cagtatatta atttatatat tatattacaa gtattttcaa
      721 gtcaaaatct ttctaaaaaa tttaagaatt attaattcct catagaaatt atcattggta
      781 catagaagtt tttacataca attatacaat aatttaatta atagttacat aaaaaagtaa
      841 tactttaata tattttgaca ccatattgac attagaatta attcatgata ttctgttttc
      901 gacaaaacaa cttctaaaaa taaaggagat atatttcatg gattattcaa atgaactaaa
      961 agagctcttt ttaatgaatc aaacatatgc aactcttttt acccttacaa acaaaataca
     1021 aatagaagga gataaatatt ttggtatact tacttcaaga cagtatatga ctattttatc
     1081 aattcttcat ttaccagagg aagaaacaac attaaataat attgcaagaa aaatgggtac
     1141 aagtaaacaa aatataaata gactagttgc taatcttgaa aaaaatggat atgttgatgt
     1201 aattccaagt cctcatgata agagagcaat taatgttaaa gttacagact taggaaaaaa
     1261 ggttatggta acatgtagta ggactggaat aaactttatg gcagatgtat ttcacgaatt
     1321 taccaaagat gaattagaga ccttatggag tttactaaaa aaaatgtatc gttttaatgg
     1381 tgaagaacaa gatggctttg aagaagatgc taattttatg gaatatgaag aaatagataa
     1441 aattaaatct gaagcacttg aagaatttgc aaaaagaaga aatagggtaa ataaaaacga
     1501 ttaatactat cgcatctata ataccacttt tttatagtac aatattttat ataactataa
     1561 tgtcattttt attttttaac aaaacaatca ttacatagat taggtgttct taggaactct
     1621 ttttctgttt tttgtacaaa aaaaggagat gattagactt ttattttaaa gcatctcctt
     1681 tttcgctatt atgattattc tactcctaaa atcatatctt ttaatttttg taaatcatta
     1741 attggttgac tacaactatt actttgacac acgtaataac tagtcttatc atccttcaat
     1801 ctatagttat ttaaaaaacc tataatagtg ttttcttcat tgtatttctt tgctaaaaaa
     1861 gttgcatttg gtataaaatt ttcacttatc aattctttaa atgcaattaa atcagaatct
     1921 tccttaaaaa tacatataat ttccttagta gaataaagtt caaacatcaa tgaaagcata
     1981 taaaagctgt agccagttgg agaactcttt acattatcca catacagttt taattgttta
     2041 tatgacattt cttcaagtct attatctcca gtaattttcg caagtcttat aagattgtat
     2101 aattgaacag aatttcctga tggaatagct ccatcataca aatcttttgg tcttgctatt
     2161 aaattttcac tatctttacc atatatataa aatccacttt tttcataatc ccaaaaaagg
     2221 ttgatacagc tttcattcaa atttaaagcc ttttctaaat atttcatatt aaatgttgac
     2281 tcataaagct ctatatacgc ccagattaaa aaagcataat catctaaata tgctaaatag
     2341 tcggaacttc catctctata tctagctaat aatctacctg actcatttac aagattatta
     2401 tttataaagt taagacactt atttgaatac tctaaatata tgtcattttt taaagtacta
     2461 tatgcctttg ttaaagcaac tatcatcaat gcattccatg atgtaagtat cttgtcatct
     2521 ttatgcaaag atgttctttc ttttctatat tcaaatacct ttttactaag gtcagctatc
     2581 ttttcattat gtctttcata ttctttattt tttataagat taggaatact tttaccttca
     2641 aaatttccac tactagttat atcaaaataa ttattaaaga aagttccatc ttcttcaccc
     2701 aaaacttcaa ttatctcaag aggattaaat gtataaaatt taccttcttc accttcacta
     2761 tcagcatcct gtgctgaata aaacccacct tctttatcct tcatctctct aactacataa
     2821 tctatagttt ttatagcaat ttctttatat aactctttct ttgttatttt atatgcatct
     2881 aaaaaagcta tagtaagcat agcattatca tatagcattt tttcaaaatg aggagccaac
     2941 cactttttat cagtagagta tcttgaaaac ccaaatccaa tatgatcaaa caagcctcct
     3001 ctatacattc catctaaagt tttttccacc atttttaaca catctttatc tttttctatg
     3061 ctataatatt tcattaaaaa cataagattc tgaggacttg gaaatttagg agcattacca
     3121 aaaccaccat acttttcatc ataaatggcc ttaaatactc taactgatga actcaacatc
     3181 tcttttgata aatcaccctc agtatttttt acaccaaaat catccttaag agcttctatt
     3241 atttcatctc cagattttat taatatatcc ctacttgtat tccatttttc agatacattc
     3301 tttaataaat ctataactcc aggtctatta taccttgaat attttgggaa atatgttccc
     3361 gcaaaaaaag gttttttatc tggtgtcatt attatggtca ttggccatcc accactacct
     3421 gtcatagctt ggcaaactgt catatacacg ctatctacat caggtctttc ctctttatct
     3481 acttttattg ccacaaagtt tctattcatt atttcagcaa cttcttcatc ttcaaaagat
     3541 tctttttcca taacatgaca ccaatgacat gttgaatatc ctacacttaa aaatattggt
     3601 ttatcctctt cttttgcttt tttaaaagcc tcatcattcc aactatacca gtttatagga
     3661 ttataagcat gctgtaaaag atatggagat ttttcattta ttagattatt aggttttcta
     3721 ttactgtcca aaataccacc tcatttgtta ttttattata tgttttgcaa taaacaaata
     3781 gatatacact tataataagt gtatacccat ttttaactat acatatttaa tttttcatat
     3841 aattaattct tctagtaaat aatttttata actactaaaa caaaacaaca taaaaaatat
     3901 caaattattt ttttaaaggt ttttcaaaag aaggaggatt aatatctatc ttagtattgt
     3961 gatttggccc tggagtagac aaatcgaaat ataatctaga atcttctgta actccaaaat
     4021 ctctatttga acctgtatct ttaactttat ttaaagcatc tctaaataga gcattatgtg
     4081 cttcttctct gttaagtaag aaatctattg tttcttttac atatttatca tctatttgtc
     4141 tatacaagta ctcataaact accttagctc tttgttcaga agctatattt gaaagtaagt
     4201 cagctactaa atcccctgta actgtaacat aattagcagt ccaaggagct cctgatgagt
     4261 ttattaagac tggtgataga ccagttaata catgtgtttc tatttcgcct gtattaactg
     4321 cattataatc aacatcatgt ccatttaata aatttatagt ttgtgctacc atttccatat
     4381 ggctaagctc ttctgctgct atatcaagaa ataaatcttt tatttgaggg tcttttattc
     4441 taaaactttg ggacaagtat tgcatagctg cttttaactc tccatttgca ccacccaact
     4501 gttcttgcat taaaacagca tattgaggat ttggtctttc tacttttact tcttttaata
     4561 atgctttatc gtgtttaaac atatgaatac ctccgtaaat tattattcaa aattattatt
     4621 tacttaagtt gtaatattat tctaggttat ttgtttattg ttgttatttt ttacaagtgt
     4681 tggtatttca atgttttatt aaggtcagtt ttgttgttta tgttttatta tgtttaattt
     4741 aaatttattc tattatttat tttcggtggc aaaatggtgg caagtttggt tttgtcacca
     4801 tttattttta tttgtaaaaa atatagttta tcaatataac tttattgaaa aatatgtaat
     4861 atttattgac tttcacgtca cgtgatgata taatataaat atagaaagga ggtaagaaag
     4921 tggggagatt agaaagaaga aaaaataaaa gagaaaacaa atttaataaa attaaaaatg
     4981 ctttctcttt cacattagtt ttaattaatc tagtactcgc aatactaaga ttgattaaag
     5041 aattataagt ttcaatccta ggaggaaaat accttccttc taggtagctt ctttctaata
     5101 ttataacaca ttttctaatt taatatgaat aataaatttt atagaatttg tatgattatt
     5161 cttggtatca ctatagtttt aaatattatt aatataataa taagttttac ttttaaagct
     5221 cttattgctt tggtatttac tttattattg ctggcattta ttaatagtag aaaggaatga
     5281 tattattgga gcaaagaaaa cttagaattg gatttaataa aagtggcaaa ggctctttta
     5341 cacccagatt gatacttcca atgtcatgga ttaaagagat gaatatctct cctgatgaaa
     5401 gggatgttct tgttactttt gaggatggaa aaataattat tgaaaaaact gaaaatgaat
     5461 aaaaaaggag gtagtaacta tttccagtta ctacctttca atctatttaa ctaagttttc
     5521 tacttcatta aatgcttttt catttccatt tccaccaatt tgagttatat ttttaaactt
     5581 tttattcttg attaactctt tttggctatc atctaatcca tttccaacta atactactgg
     5641 agattcagtt ttacctgcaa gcactcctac tgataaggca tctactaaat cgtcttgttt
     5701 attcattcca ttcttagcta catataagtc attaagtgta tctttgctat agaaatgact
     5761 tattactttt gagttagttt ctgttctatc agaacctgct attttagtta cagatggtaa
     5821 tgtattattc acttctttat tattaaataa agattctcca cctattacat atgattttga
     5881 tatgttttta ttagctatta agtcttttat atctttcata tcatcatttt gattagtaag
     5941 aagaattggc atttcctctt ttgctgatat agctcccata cttactgcat cagctagtcc
     6001 cttttctcca tttaccacta ctacttttga caggttagaa tttttgtcta attcttttgc
     6061 aagttttatt gatgtttcat atctatcatt tccagatatt ctaattacat ttaatccttt
     6121 tttctttaaa tcagacacta ctttttcatc aacagacttt aatccaccca ctatgtacac
     6181 attttttgct tctaatctat ttatttcttt ttctgttaat ttatttaaat tattattttt
     6241 agtaagtaat attggtgcat ctttagactt agcaaatggt gttgctgata aagcatccga
     6301 tatgctagag tcatttatta acactatatt atcagcttta ttccatcctt tttgacttat
     6361 tttcactgat gtttcatttc tgtctgcacc tgttattttt tccttagatg gtgctatatt
     6421 aaaatcttct ttacttgaag ttccaccaga agaaccactt gatcctgttg attttctttt
     6481 ataagttgct ataaaatcag tactatcatc aaaaactaat ttacttacct gctctgatgt
     6541 atatattttt tcatctttat tgttcctcca accactaaac tcatagtttt ctctttgagg
     6601 attaatttga atttcattta tactagaatt atctgatatt tctttttcat taattatact
     6661 tccatcataa tctatatatc tgattctaca tttcactaat ttatctattg atatatttac
     6721 atccatagtc tctgtatcat ttactggacc atattcatat tccaaactat aacttatgct
     6781 atttggaaag tcagaaagat tttcccatgt tagaaaacct gttgaaatat tataattttg
     6841 tggatataat tcctttaatt tcgaattttc aattcctata tctacaacta tattttcacc
     6901 attttttata atgctttttt ctatatattg accactacat tccagtgaat ttaattcttt
     6961 atttttactt atatctattt ttgttaaact atttttccca caaagtaaag aatctaattt
     7021 tatatttgta gatacatcta aatcttgtaa tttattctta gcacatccca aatactcaag
     7081 acttgtaagt ccttttatat ttaatttact caggttatta cctgaacaac ttaaattttt
     7141 caaaaaaaca ttattactta aatcaaggga tttaagttta ttgttagtac aatttagaga
     7201 tttcaacttt ttattatgac ttaaatctat actttttaaa ttattattaa gaaattgtaa
     7261 attttctaag ttcacattat tacttaaatc tatctctgtt aattcattac tagtaaaaaa
     7321 taaacttacc aaatttttat tatttttaag
//
LOCUS       DAELXX010000152         7338 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.154, whole genome shotgun sequence.
ACCESSION   DAELXX010000152 DAELXX010000000
VERSION     DAELXX010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7338
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.154"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(208..2136)
                     /locus_tag="KPH29_003359"
     CDS             complement(208..2136)
                     /locus_tag="KPH29_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG1468925.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKYNLILVSKPYYGVKVEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNVDLIELQRVVLSEINKFNINFLDFNLK
                     NFIIHLAITISRILDGYCLDNVLDVVLTDFQSNTTVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRVDTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKS
                     ILNSKSYNLNKVNPLINTIKSNYPLAFEITLNAIEKVFKNSIYSLTEDEIGYVSLHIG
                     AGIERFFQNNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASEL
                     SDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENISKSITNNSHIYSNLLDNFFDKNLF
                     IVNPKIKDKDELIKLMCNKLTQSEIVFPSFAESVFYRESLSSTNIDDFLAIPHPMELS
                     SIRTKICISILNEPIYWSEDSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDS
                     ISNCNDFDNFLSIIKSIV"
     gene            2370..2822
                     /locus_tag="KPH29_003360"
     CDS             2370..2822
                     /locus_tag="KPH29_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG1468926.1"
                     /translation="MVLQEKYIFIDKDLKSKEEVLNFIADSAYILGIATNKEALLKDL
                     LKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HKNEGNIHLKTLSKLATCLMEDDFTSEVKNASDASHLVKYITNKMKEE"
     gene            2825..3133
                     /locus_tag="KPH29_003361"
     CDS             2825..3133
                     /locus_tag="KPH29_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBG1468927.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            3247..4353
                     /locus_tag="KPH29_003362"
     CDS             3247..4353
                     /locus_tag="KPH29_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBG1468928.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAM
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENTEFDEIELDIM"
     gene            4428..7106
                     /gene="mngB"
                     /locus_tag="KPH29_003363"
     CDS             4428..7106
                     /gene="mngB"
                     /locus_tag="KPH29_003363"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBG1468929.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDEYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIGKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISNIENYIDSVKNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTANEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGEILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESSKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MILKYSLTIPASLEERSIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLLFAFNDEKQ
                     IYENEFSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNNSKLKVNTKTVEVNSLSHCKVQTIVIK"
BASE COUNT         2920 a          919 c         1068 g         2431 t
ORIGIN      
        1 ctcttttttg tatgaaatca aatatatttt tatcatttta tcaatgaaaa acaagtttct
       61 ctctctattt tgagacagaa tctttattgt ataataatta taatcaatac aaaataataa
      121 caaattatac aaaacttact tatactattt atgctaaaaa caaaaaatat tttattaaag
      181 tttttaaaac ttatgtaata taaaaatcta tactatagac ttgattatgg aaagaaaatt
      241 atcaaaatca ttacaattag atatactatc tctaatatca ttgtcatgaa ttatctttaa
      301 aaggatatca taaatggagt ttatttttat ataatcatct ttatttatag ctaacatcat
      361 gattaattta acagtagaat cttcactcca atatattggt tcattcaata tggaaataca
      421 aatcttagtt cttatagagc ttaattccat aggatgaggt atagctaaaa aatcatctat
      481 attggtagaa gataaacttt ctctgtaaaa tacagattca gcaaatgatg gaaatacaat
      541 ttcactttga gttaatttgt tgcacatcaa ctttataagc tcatctttat ctttgatttt
      601 aggattaaca ataaataaat ttttatcaaa gaaattgtcc aaaagattac tataaatatg
      661 ggaattatta gtgattgact tagatatatt ctctatatct ttttttaata gttttaaatc
      721 cactaataca ataggaatac tatcatgatt taaaggaatt gtcgatatta ttatatctac
      781 atctgacaat tcactagcta aaaattgatt aaatgataga cattgaagta tatttatttt
      841 atcatgaaaa actttgttta attgaacctc taataaccta cttgaaccat accctgaacc
      901 acatactaat actacgtttt tacacttaat attattttga aaaaatctct ctattccagc
      961 acctatatgc agggatacat atccaatttc atcttctgtt aaagagtaaa tactattttt
     1021 aaaaactttt tctatagcat ttaaagttat ttcaaatgct aaaggataat tactctttat
     1081 tgtatttata aggggattca ccttatttaa attataagat ttagaattga gaattgattt
     1141 aaagtgcagt actaaatcat caaataatac actatcattt ctaaggtcaa aagtatattg
     1201 attgttgatt acttctaaaa tttcttcaac atactctata attttagtat ccaccctatt
     1261 ggaaacgtta tccaaaagag aacttttagt tataaaatga ttatatagat aaactctgtc
     1321 tgcctttgaa ataattatag tatatttact ctcaatataa ttaaatatat tttcaacagt
     1381 tgtattcgat tgaaaatctg ttaaaacaac atctaaaaca ttatctaagc agtaaccatc
     1441 aagtattcta cttatagtaa tagctaaatg gataataaag ttttttagat taaaatctag
     1501 aaaatttata ttaaatttat taatttcact taacactacc ctttgaagct ctattaaatc
     1561 tacattatta aataattcta tttctctatc tgtaattcca attatatagt tttcaaaatt
     1621 cctatttatc atattattag aaatacatct tcgaatatct atttccttcc cttcaacttt
     1681 aactccataa tatggcttag aaactaatat taaattatac tttgacaaaa taggctttat
     1741 tctttttatg tcatttagta ttgtaaattt actcacatac aagctattag ctaaatcttc
     1801 tagttttata tatgtattgt tatagagaat atattttaaa atatattgat ttctttctat
     1861 aggagaatct ggtatttcac ttgaatccat aatatcatca gatattaatg ctagatactg
     1921 ttggtataat gctaaattaa gaatatctat ataatatcct tctccttttt ttattttaat
     1981 aatagcacca tttttctcta aagttccatt tatagaagta atatcatttc ttatagttct
     2041 ctcactcact tttaaatgat tacttaaagt tttacaagta attttatcat cactttcatt
     2101 taataattta aacaacgtga gcaatctctt gtgcataaat atcccccctt atagaatttc
     2161 ttctaaatta tactatttac actcatataa tacaattact ctaataaaat tgtattataa
     2221 taatttcttt ccacttctat atggaaatta gttattatca taatataaat ttccatgtcg
     2281 aagtggaaat tagttatatt taatttaatt aggataatga ttacaatata aatataagtt
     2341 aagacattac gaatggagtg gaaaaatata tggtactaca agaaaaatat atatttatag
     2401 ataaagactt aaaaagcaaa gaagaagtat taaattttat tgcagatagt gcttatatac
     2461 taggtattgc aactaataaa gaggctcttt taaaagacct tctaaaaaga gaaaatgaat
     2521 ttccaacagg aataaaagat ggatttgcga tacctcatac aaaaagtgta aatgtaaagg
     2581 aagcttccat actctatatt aaaacaacta acgagttaga gtggaaaact ttagatgata
     2641 gtaaggttag atatgtattt agtttgttag ccccacataa aaatgaagga aatatacatt
     2701 taaaaactct atctaaatta gccacatgtt taatggagga tgattttaca agtgaagtta
     2761 aaaatgctag tgatgcaagt catttagtaa aatatataac taataaaatg aaggaggaat
     2821 aattatgaaa atagtagcaa taacatcatg tatagcagga ttagcacaca cacctatggc
     2881 agcaaaagct ttagaacaag ctgcatcaaa aatgggtcat gaaatcaaag tagaacaaca
     2941 aggtgctatg ggacgagtaa atactataac aaaagaagaa gcacaagatg cggactttat
     3001 acttatagca tctgaccaaa ctatatctgg aatggaaaga tttgatggta aaaaagtgat
     3061 aaaagtcaaa ataggtatag cgttaaaaaa accagaagct gtcttaacaa agtgtataga
     3121 agctatttct taataaaaaa atctcactct aatatagaat gagaaaaata aaaaattaaa
     3181 aaattgtcta atgtaattat aacatattat caaaaaatat aacaaactta tataggagga
     3241 ttaaaaatga gtaaaaaaaa taaatctctt ttaagtgaac ttaagggaca cttaatgaca
     3301 ggtatctcat atatgttgcc acttgtaata ggctcatctt tagttgtagc tatcccaaag
     3361 cttacagctt tagcaatggg tataacaagt ttagatgctt ataaaaatgt atctggtttt
     3421 tatcatattc tttacttaat ggaacaagtt ggttggactg gcataggtct attaaatacg
     3481 gtattagcag gatttatagc attttcaata ggtgagaaac cagctatggg tgcaggtttt
     3541 attggtgggc tcatagcaag taatactaat gcaggattct taggagcagt aatagcaggt
     3601 ttctttgctg ggtatgtttg taagttttta aagaaaaaaa ttaaaatatc tgggtcagca
     3661 tcaggaatga tgccacttat aataatgcca cttataacag ttggattaac tggtttttta
     3721 atgagtgttg tattagcagg acctttagga aacataaata cttcattaaa tgcttgggta
     3781 gctgaaatgt gtcaaaatgg aacaaattcg gtggtattgg ctttaatttt aggcgcaatg
     3841 ataggattcg atttaggtgg accagtaaat aaagcagctt ggatggcagg aaatgcatta
     3901 ttgttagagg gaatctatct accagcgata ttggtaaact gcgcaatagt tatacctcca
     3961 ctaggatatg gtttagctac attcataaga aaatcaaaat tttccaatgc attagctgaa
     4021 acaggaaaag gcaatatagt tatgggtata attggaataa cagaaggtgc aattcctttc
     4081 actttaacaa atccattaaa attagttcct gttaatatga taggatgcgc tttgggtgta
     4141 ggattatcag ctttgttagg tgtacatgct ataatgcctc cagttggtgg attatatgga
     4201 tttatatcaa taggttctgg atgggcttac ttagtaggtg caatatttgg agctttagta
     4261 atagcaatat tatcaacaac tttagtagac ttcaatgaag atgaaactac ggaaaataca
     4321 gaatttgatg aaatagagtt agatataatg taaattaaaa acttaatttt agataattga
     4381 gaattgagtg tactctcaat tctcaattaa attttgggag gatttttatg aaaaagacaa
     4441 aagtacatgt agttccacac agtcattggg atagagaatg gtattttaca acatcaaggt
     4501 caaaaatata tttaatgaaa gattttcaag atatattaga catattggaa gaaaaagagg
     4561 actttaaata ctttactatg gatgcacaag cttctctctt agatgattat ttaaaatgga
     4621 gacctcagga tgaatataga ataagagatt tagttcaaag aagaagatta gttattggac
     4681 cttggtatac tcaaacagac caattggtta tatcaggaga atctataatt agaaaccttt
     4741 actatggaat agatagatgc aatgaatttg gtgaacctat gagggttgga tatgtaccag
     4801 attcttttgg gcaatcagct caaatgccac aaatatacag aggttttgga atagatagtt
     4861 cattattttg gcgtggagtt tctgatgata tggtagatac aacagagttt atatggaaag
     4921 gttctgatgg ttcaaaagta ttagcagttc aaataccatt tggttattac tatggaggta
     4981 atataccaga agatgattca gatttaaaag aatatttaaa agatataatt ggaaaactta
     5041 aggttaaagc ttctactaat aacgtatatt ttccaaatgg atttgaccaa gcaccaataa
     5101 gaaaaaacct tcctgatata ttaaaaaaag ctaatgaaat agataatgaa aatgaatacg
     5161 aaataagtaa tatagaaaac tatatagatt cagtaaaaaa tgaaagaaat gaatttacag
     5221 agcttaaagg tgaattttta aatgctaaac atatgagaat acacaaatcc atattctcct
     5281 caaggtcaga ccttaaaata atgaataaca aattagaaaa ttatatcaca aatgtacttg
     5341 aaccattatt aacattaagt tattcacttg gaaatgaata ccctcatcta gttataaaag
     5401 atatatggaa acttatgttt gaaaatgcag ctcatgacag cataggttct tgtaatagtg
     5461 acacagcaaa tgaggatgta tacacaagat acaaacaagc cagagatttg agtataaatt
     5521 tattagattt gcatatgaga cttataagta ctcaaatcaa aaataataat gaagaaataa
     5581 cattaacagt attcaatgga ttcccagaag tcagaaatga agttgtagaa tttgacactt
     5641 atattccagg tcaagatttt gtgataaaga ataaaaatgg agaaattctt aattatataa
     5701 taaaagaaaa agaagatatt acaaattata tattaactca aacaataaga ttaaacccaa
     5761 gtaaaaaaat atatataccc aaaactgtat atagagcaaa aattgcactt tcagtaaaag
     5821 atataccacc aatgggatat actcaactag tttttgaatt ggatactaaa aataaatgtg
     5881 aaatagaaac aagtaattgc aaagaaatag aaaacaaata ttacaaaata acagccaata
     5941 aaaatggttc attagaaata ttagataaag aatctagtaa gacttataaa aaccaagctg
     6001 taattgaaga caatggagat gatggagatt catacaacta ttctcctcca agaaaagata
     6061 tttatgtttc atctttagat tctgtatctt cagtagaagt gctaaaatca aatattcaag
     6121 aagaaatgat acttaaatat tcattgacca taccagcttc actggaagaa aggtctatag
     6181 gtaaagtttc tgtgaaaatg ccagtaacaa tgaaaattac cttagaacct aaagaacaaa
     6241 taattaagtt taatgttaat ataaaaaata accaagcact atctcataga gtaagagttt
     6301 tatttaatac agaaatagca tctaagtttt ctatagcaga tcaacaattt ggaataatac
     6361 aaagaccaac agtattagaa aaagaccttg cattatggaa aagagacaat tactcatggc
     6421 aagaaaaacc tataactatc gaaccaatgc aaagttttgt tactttagag gatggacaaa
     6481 gaggaatagc attaataact ggttgtgtaa gagaatatca aatagttggt gataatttag
     6541 atacaatagc attaacacta ttcaggtcat ttggatatat gggaagagaa aatttacttt
     6601 atagaccagg aagagcttct ggagaaaaaa taatagcaac accagatgca caattactta
     6661 aagagttaaa ctttgacttt ggtctttaca tatataacaa taaatttgat gaagcaaatg
     6721 tagctaatac agctaagaga ttcttaacac ctatacaaat atatgaatat gctgattttt
     6781 taaatggtag acttctattt gcattcaatg acgaaaaaca aatatatgaa aatgaattta
     6841 gtttgatgaa tgtaaataat tcaaatttca cagtaagtgc aataaagaaa gaagaaaaag
     6901 gagatggaat tgtagttaga atcttcaatg gtatgaaaaa tgaggatgct aagggaagtc
     6961 ttaacatcaa taaaaatgtc aatgatgctt atagtgcaat gctcgacgaa atctatgata
     7021 ataatagcaa gcttaaagta aatactaaaa ctgtagaggt aaattcttta tcacattgta
     7081 aagtacaaac aatagtaatt aaataatata aaatagctta tacaagattt caaaataaat
     7141 tgctagtact atgttaatag taaacccaga tattatgcta tttataagct ctgtaacact
     7201 aaataacatt ttgtaaaata aaaaggtgaa ccttgtatat taggattcac cttttttaaa
     7261 taaatcaaca ttctgtttta aaataacatt tataagctgt tttgaaataa tacttacaaa
     7321 ttattttaaa acaatacc
//
LOCUS       DAELXX010000153         6908 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.155, whole genome shotgun sequence.
ACCESSION   DAELXX010000153 DAELXX010000000
VERSION     DAELXX010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6908
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.155"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(293..955)
                     /locus_tag="KPH29_003364"
                     /pseudo
     CDS             complement(293..955)
                     /locus_tag="KPH29_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440163.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            complement(1142..1651)
                     /locus_tag="KPH29_003365"
     CDS             complement(1142..1651)
                     /locus_tag="KPH29_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBG1468930.1"
                     /translation="MIGLKCGMVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEEAKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNSVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLE"
     gene            complement(1726..2139)
                     /locus_tag="KPH29_003366"
     CDS             complement(1726..2139)
                     /locus_tag="KPH29_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG1468931.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKPCAGCLNSDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCGGIISIHDRECSECQEKVK"
     gene            2576..3256
                     /locus_tag="KPH29_003367"
     CDS             2576..3256
                     /locus_tag="KPH29_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HBG1468932.1"
                     /translation="MDEKILELLQEIQGNISNMQGSISGIEGTVNGMQGNINYMQGNI
                     NYMQGTISDMQGTISDMQGTISDMQGSINGIEGTISDMQGSINGIEGTISDMQSNIND
                     IEGTISDMQGSISSMDGTINSMQDSITDMQGEIKEVNSRLDNMEPDIKDLKTGQVETS
                     SRLDTIQSDVTEIKSDVKINRNTLDTVIKHTSSLVEDTSDIKNDLNNIEIVTSKNWND
                     IAKLKSVK"
     gene            complement(3501..3656)
                     /locus_tag="KPH29_003368"
     CDS             complement(3501..3656)
                     /locus_tag="KPH29_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468933.1"
                     /translation="MVKFNQTLKVSKEKIITLKQEDINGKMKTRFYPFISDKGICFNQ
                     RWTFILG"
     gene            complement(3863..4042)
                     /locus_tag="KPH29_003369"
     CDS             complement(3863..4042)
                     /locus_tag="KPH29_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG1468934.1"
                     /translation="MLQTGKQGADKKYLDNMFFVKGYVEELLFLDNSYDIVFSCIVFH
                     HCTNVQNIFCIFWKW"
     gene            complement(4267..4800)
                     /locus_tag="KPH29_003370"
     CDS             complement(4267..4800)
                     /locus_tag="KPH29_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBG1468935.1"
                     /translation="MQYISYYHSPIGNILLASDNVGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGEEPNFTPSLHMVGTPFQTTVWKILQQIPYGKTITYGEIA
                     HKVAQQKGISKMSAQAIGGAVGHNGISIIVPCHRVVGTNGSLTGYAGGIDRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            complement(5618..6190)
                     /locus_tag="KPH29_003371"
     CDS             complement(5618..6190)
                     /locus_tag="KPH29_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG1468936.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGSCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            complement(6252..6515)
                     /locus_tag="KPH29_003372"
     CDS             complement(6252..6515)
                     /locus_tag="KPH29_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBG1468937.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDLYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            complement(6515..6736)
                     /locus_tag="KPH29_003373"
     CDS             complement(6515..6736)
                     /locus_tag="KPH29_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBG1468938.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYSNPKNRVRAKKGGNMY"
BASE COUNT         2389 a         1192 c          864 g         2463 t
ORIGIN      
        1 gaggaatagt cgttgaagtt tctcctattc ggagtttacc tgctgattgc ccattttaaa
       61 gtacttagga tttaacctta taccatctaa ccaatttttt ctactttcgt aactttcaca
      121 cttaggttta tttcatcctt atgttttagt ttggttagct ttaggggttt ccagcaattc
      181 aagtagtatt ggataggttc tatcctatct ctacatatag gtttccctat atgctgacta
      241 ttttagctac taactcatga ctaaagtcac aagtgtgcgt agctattttt taatcaaact
      301 ggaatacaga ctccattcct aggaaattgg ctgaaatata gtctgctgtc aaagatgctg
      361 tcatcacaga aattaaaaca gatactgaaa agtttttatg gacttcttca agtgaaaaca
      421 taacacctgc cagtggtgca tgaaaggctg ctgataatcc agcacttgcc ccacaggtta
      481 acaggaattt ttcctctgtc ttccctcggt caagagctct agaaatacct ttacctgcca
      541 tagcaccaag ctgaatggaa ggaccttccc ttcccaaagc aagaccaccc ataagtgata
      601 agaatcctcc aataaatttt gtgggcaaca ctcgccacca tacttgtttt attttccctg
      661 tcatttcccc ctcaagctga gggataccac ttccagaaat cattggctcc cagtccacca
      721 actttgatac aatccacgca agtactgcaa gtactgcaaa ccaagctatt atttttattg
      781 tagaaccttc tacaaatttt aaaatttggc taagccaagt accagcctgc cctaaaagta
      841 tcctgtataa aagcacaatc aaaccagcca cagccccaac cataagtcct tcaccaatca
      901 aaattacctg aaagtgctct gcccgtttga taatatgaga tgtatccttt ttcatactgt
      961 tcctccttca tccaataatt ctacacaatc tgaacctcca atgactaaag tcatgagtgt
     1021 gtacagatat tttttaatca acaaaaatag atttttattt actaaatata agaaaataat
     1081 tgttcaattt ctcaataaat gtaaatttat tcttttaggt atttattctc cacagtcgtg
     1141 cttattccag caaaatattt attagttctt gctttctctg tgtatagatt ttacgattgt
     1201 cctcaaactg taatgctaat ttttgtttat gactatcata gagttttgct ttgtctgtaa
     1261 aagtatttag ataatctcga aaattgatat aattattcca tgcgacacta ttccattcaa
     1321 caacatgaat atggtgagta cgagtatttt tctcaaaatc tcccatagca aaaagtaatt
     1381 gttttggtac atcctgacct cgaaaaacga ttccacattg ctctaacctt tcgaggtata
     1441 atttagcttc ctctaaagaa gcaacaccta caacaatatc aataataggc tttgcgctaa
     1501 tactgggaat agaagtgctt ccaatgtgtt gaatatcaat ggcagtctta tcccatatac
     1561 ttttcaataa tataatgaca tcttttgcac ttttatccca tatcatctga tgttccaata
     1621 atttaaccat accacatttt aaacctatca ctatatacca cctcaatata atccaccttt
     1681 atatatatat tacaattttt tctatcttga taaacagtga tctagctatt tcactttttc
     1741 ttgacactcg ctacattccc tatcatgaat agatataatt cctccacatt tagaacaagt
     1801 atatctctcc ttctgctttt gcataaactt ctctaaaccg tactgctgaa caaatttact
     1861 attttccata aggcttgctt gatatctctt gttataactt ttttcaagat tttttatgag
     1921 tttacaagga aaattgaaac attcaaaaca ataaaacaac cctttttgac agacacaatc
     1981 ctttatttta cacttacggc agtgttctgg ctttcctgta tcgctgttca aacaaccagc
     2041 acatggcttt ttgtgataac aatgcttata gcaaaccata caattcattc cacaaggagc
     2101 aaacataatc gtatcaatac tctcttttgg cattttcata acattttact cctttcaaaa
     2161 atccaatttg tcgaattgtt atatattcat attgtaacat aaaaaatagc taaaaaattc
     2221 atgcactttc attgccaata aattttcaag gaaatatgaa agtttaaaac taagaataaa
     2281 aagaaataaa aaggaagcta ctaatcaaaa tattcaaaca caagtaatca attatgatat
     2341 taccagcagt tttaaataca aatataacca catatgcctc aaaaacactt attatagtat
     2401 cttagacact tagacaatac acaagattca ttaataaaaa agttctcaat atagatatat
     2461 ttaaccatgt tttgaatagc agataattat atgattaaaa attaaatatg acatagcgtt
     2521 tgtggaatat aacacctaaa ttatgttata ataataaaac aggagtgtga tagtgatgga
     2581 tgaaaaaata ttagaattat tacaggaaat acaaggtaat attagtaata tgcaaggtag
     2641 tatcagtggt atcgaaggta ctgttaatgg catgcagggt aatattaact atatgcaggg
     2701 taatattaac tatatgcaag gtactattag cgatatgcaa ggtactatta gcgatatgca
     2761 aggtactatt agcgatatgc aaggcagtat caatggtatc gaaggcacta ttagcgatat
     2821 gcaaggcagt atcaatggta tcgaaggcac tattagcgat atgcaaagca atatcaatga
     2881 tatcgaaggt actattagcg atatgcaagg cagtatcagt agtatggatg gcactattaa
     2941 tagtatgcaa gacagtatta ctgatatgca aggtgaaatt aaagaagtca atagtagatt
     3001 agacaatatg gaacctgata ttaaagactt aaaaactgga caagttgaaa ctagtagtag
     3061 acttgataca attcagagtg atgttacaga aattaagtca gatgtaaaaa ttaacagaaa
     3121 tacattagat acagtgatta aacatacctc ttcattagta gaagatacat cagatattaa
     3181 aaatgattta aacaatatag agatagtcac atctaagaat tggaatgata ttgctaaatt
     3241 aaaatcagtt aaataatata ccatagtaaa aaatacctga aaatttcaca tacttcttta
     3301 aagttatacc aaattttata cagaaatact gattatccga caacacactt tacttataaa
     3361 taatttatta aaaaatactc attttatcct aaaattaaca cgactaaatg agtcttgata
     3421 taataataaa aaataaggga gattgaacac taaaaagttg tgtatctccc ttatattcta
     3481 taatatagtt atataatttt tcagcctaaa ataaaagtcc atctttgatt aaaacatatc
     3541 cccttatcac ttataaaagg ataaaatctt gttttcattt ttccatttat atcttcttgc
     3601 ttaagagtaa taattttttc ttttgatact ttaagtgttt ggttaaattt aaccaatcga
     3661 ctaagttttg ttcaacatct atttttcata ttttccaata cttaatctat tttttataaa
     3721 taattctttt atttctaact cacttacatt ttttacataa aacgaagcat ataaatttca
     3781 accttatctt gtatggttct taatacttaa tgagtagctt aagtatcaat cattataaat
     3841 tttcccctta tatttaacac tcttaccatt tccaaaatat acaaaatata ttttgaacat
     3901 ttgtacaatg atgaaataca atacatgaaa atacaatatc ataactgtta tctaaaaata
     3961 gaagttcttc aacataacct ttcacaaaga acatattatc taagtatttt ttgtctgctc
     4021 cttgttttcc tgtttgtagc atataaatta ctgcatctaa acatataact actcacacga
     4081 aaaaagaaaa taaacttcca aaatacaagc tctaaaacta cctctaaagt tatataatgt
     4141 ttactaggaa taaggttgta taacatatag tttaaagaat agttttttaa aattgcaagt
     4201 tattgctttc aaaattagat gtttatatat caaataaatg attaagaatt tctaaattta
     4261 atggctttat aatgcactcc ctttctttgg gataaaataa tgttgcacag atactttttc
     4321 taaagataac aattttattt ttctatcaat tccaccagca taccctgtca aactcccatt
     4381 tgttcctaca acacgatgac atggaacaat aatagagatt ccattatgcc ctactgcacc
     4441 accgattgct tgtgctgaca ttttagatat tcctttttgt tgagcaactt tatgtgcaat
     4501 ttctccataa gtgattgttt taccatatgg tatttgttgt aaaattttcc acactgtagt
     4561 ctggaaagga gtacctacca tatggagtga tggtgtaaaa ttgggctctt ctcccgaaaa
     4621 ataaatatct agccatttct ttgtctgctc gaaaattggc aattcttttt cttgatgttc
     4681 ttggtcaaga ttataagcat aatacttttg attctcaaac caaagaccag ttaacccaac
     4741 attatctgat gctaaaagaa tatttccaat tggactatgg taatagctaa tatattgcat
     4801 acaattcctc cttatattct gaaaacttca ctcaaatcat tagacacttc ttttctatct
     4861 gccattatgt aaaaacatac taatataatt tccttctgac tacactttcg ttataatatc
     4921 tatcctcata tcatattagt tttatattta cctgaatctg ttcatccttt ttttcctttc
     4981 cctttgatgg atataaaatt catacattta agcttcctac tgtattaact tttatagaag
     5041 tgtatattat aattttatca ctaaatagac aaaaaaatat cgatataaca tcaagaaata
     5101 ttttactgag tttcgcttat taaaagcaac tgaatttcta aagggtacag atgagctaat
     5161 aagtgatatt gatataaaag ttgtttttca ttaagtaatc tattttagta aatgttttaa
     5221 agaaaaaacg ggatattctc caaaagtgta cagaaatata aaaaaagaga tatgattttt
     5281 tatttctttt gattatccaa agagtaaata aaaaatattg taaaaaactt actaacaacg
     5341 gcaatgcttt gaactatggt aatactttga acttttgatt ggactttttt tgctaaaata
     5401 atatatttaa atagcaaagt aatctgttta atcaataaaa aataagagat aaaagaaatc
     5461 aactaaaacc tttacctctt attttgatag tttatcagag ctaatattta cactcttttt
     5521 atttttgata cttcattaaa aatgatgctt attctctttt catttttaat acttcattaa
     5581 aattgatgct tactctctat tcacttttaa tacttaatta gaactgatgc ttacactctg
     5641 taaactcata aacatccatt aatgcttcac caaatctttg atagtgaact acttccctct
     5701 cacccaaaaa tctcaatata tctttcaaat cagggtcatc agttaaattt attaattgat
     5761 agtacgtagc caatgctttt tgttcagcag ccatatcctc atgtaaatca gttaccgaat
     5821 cgccaaagac attaatataa cttgtagtaa agtttactcc attagaatct actggttgaa
     5881 gaccatgtcc aaaattagca taattactac caagtccagc agccttaagc tcttccggag
     5941 tacaactgcc tattaattga tataacactg aagaaattat ctcaacatgg gccatttcct
     6001 ctgtacctat atcagttaaa agtgcgcgag attttccagt tggcatagta tatctttgtt
     6061 gaagatatct agttgcagct gccaactcac cattaggtcc tcccaactga gtcatgatat
     6121 attttgccat tctagggtct gcttgtctta tgttaactgg atgttccagt gttttttgat
     6181 aaatccacat attatcttac tcctccttaa tatatattta ataataattc taattttaaa
     6241 acttaataat tttatttata aaatgatttg tcccatggcc aaggttcaga tatccatgac
     6301 caaggatatt cacttgattc atatccaaaa ttagttaatg ctccatattt agattcataa
     6361 tcacacacag cttgtgtaaa tttatcgcag tatctattgt ataaatcaag agctttctta
     6421 tcccttggat tgttatctaa atataaattt aattctatac aggcaaattg atattctgaa
     6481 attctctcta aaagttctct tctatttaat gacattagta catgttaccc ccttttttag
     6541 ctctaactct atttttagga tttgaatata gccaatcatt atagttattt gactctatta
     6601 aatctaattc aggaaataaa gtacctcttt ttaatgctgt ttttggattg tatgtgtcat
     6661 cataaatttg acgattgatg tatggtctag caaattcaca acaatagtct tgtttatcct
     6721 cttttaaata tcccattaat tatcctcctt aatttttaga attttaaaat agcattattt
     6781 ttaaaaattg tatacttgtt ctatattata gtcagtagtt ttaataggtc tttaataaga
     6841 ttaaataatt aaataagtat ctttttttgt tcttatttta cctataaaaa ttttcctaat
     6901 taatcttt
//
LOCUS       DAELXX010000154         6693 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.156, whole genome shotgun sequence.
ACCESSION   DAELXX010000154 DAELXX010000000
VERSION     DAELXX010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6693
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.156"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1658)
                     /locus_tag="KPH29_003374"
     CDS             complement(<1..1658)
                     /locus_tag="KPH29_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1468939.1"
                     /translation="MKYLIEIAKKERLLVFIYLALGIGIELLNSFSANYYQSLIDRFN
                     NSTINFHIIFIYGTVLVTLCLLRYIDEYPGRRLEHSFFLDLKLKALEKMSKIDYLEYQ
                     MLGTGKLIQKIENGANAGRNIFFNFLLAVVRKIIPSILFSMVFIYRIDKKVMFTILIG
                     YFIVFIITNLLIKALYQIKEHILINEEKMNHFLVRGFVEMVVFRVNKRFEYEICQSKF
                     AKKEIIDSRVKMTLIHEAFFTIFSLIVTFIKMLIIVYSWKTKTISIGSIIALITLLDN
                     AYTPIAIFNVLYIQFKLDKAAYKRYTDILELNNDGQLLAGKEVNLMYGNIEFNEISFL
                     YNNKREIFNKLSLSIKSGEKVAIVGESGSGKSTLIKLLIGLLKPSEGTIFIDDYNLLE
                     MNLNSYYNYVTYVSQDSPIFDGTLRENIVFGKTIKSNDIIEVLEKVGLKNLYLKLEAG
                     LDTKLGEKGTTLSGGEKQRLALARLWFSDAKIIILDEATSALDNITEEKVMLNVMEFL
                     KDKTTIMIAHRFNSIKNVENIIVLKEGSIIGSGSFKKLLDGNLY"
     gene            complement(2581..3162)
                     /locus_tag="KPH29_003375"
     CDS             complement(2581..3162)
                     /locus_tag="KPH29_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG1468940.1"
                     /translation="MNNKKHWYDYLWIFSSIYLVLGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSV"
     gene            complement(3155..3385)
                     /locus_tag="KPH29_003376"
     CDS             complement(3155..3385)
                     /locus_tag="KPH29_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG1468941.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            complement(4710..4985)
                     /locus_tag="KPH29_003377"
     CDS             complement(4710..4985)
                     /locus_tag="KPH29_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG1468942.1"
                     /translation="MLELTKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
     gene            complement(5061..6362)
                     /locus_tag="KPH29_003378"
     CDS             complement(5061..6362)
                     /locus_tag="KPH29_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG1468943.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
BASE COUNT         2295 a         1229 c          546 g         2623 t
ORIGIN      
        1 aaatataaat taccatctaa tagtttttta aagctaccac tacctattat actcccctct
       61 ttaagaacaa ttatattttc aacattctta attgagttaa atctgtgagc aatcataata
      121 gttgttttat ctttcaaaaa ttccataaca tttagcataa ctttttcctc tgtaatattg
      181 tctaaagctg atgtagcttc atctaatata attatcttag catctgaaaa ccaaagcctt
      241 gctaatgcta gcctctgctt ttctccacct gataatgtag ttcctttttc acctaattta
      301 gtatctaaac cagcttctaa ttttaagtat aaatttttta aacctacttt ttctaagact
      361 tcaataatat cattactttt tatagttttt ccaaatacaa tattctcacg taaagttcca
      421 tcaaatatag gtgaatcctg tgacacatat gtaacataat tatagtaact atttagattc
      481 atctcaagta gattataatc atctataaat atagttcctt cgctgggctt taataatcca
      541 attaatagtt taattaaagt tgatttacca gaaccactct ctccaacaat agctactttt
      601 tctccacttt taatacttaa actaagttta ttaaatatct ctcgcttatt attatacaag
      661 aagctaatct cattaaattc aatatttcca tacattaaat taacttcttt acctgctaac
      721 agttgaccat cattattaag ttccaaaata tctgtatagc gtttatatgc tgctttatct
      781 agtttaaatt gaatatacaa tacattaaaa atagctatag gagtatatgc attatctagt
      841 aaagttatca atgcaattat agaacctata cttattgttt tagttttcca actataaact
      901 attattaaca tttttatgaa cgtcactatt aatgaaaaaa tcgtgaagaa agcttcatga
      961 atcagtgtca ttttaaccct agaatcaata atttcttttt tcgcaaattt tgactgacaa
     1021 atttcatact caaatctttt gtttacccga aatacaacca tttctacaaa tccacgaact
     1081 aaaaaatgat tcatcttctc ttcattaatt aatatatgct ctttgatttg atatagagct
     1141 tttattagta aattagtaat aataaaaact ataaaatatc caattaatat ggtaaacata
     1201 acttttttat caattctata aataaaaacc atactaaata aaattgatgg aattattttt
     1261 ctaacaacgg ctaataaaaa attaaaaaat atatttctgc cagcattagc tccattttca
     1321 attttttgaa ttaattttcc agtgcctaac atctgatatt ctaagtaatc tattttactc
     1381 attttttcta aagcctttaa tttcaaatct aaaaaaaaac tatgttctag tcttctacct
     1441 ggatattcat caatatatct taaaagacat aaagtaacta atacagttcc atagataaaa
     1501 attatgtgaa aatttatagt actgttatta aatctatcta ttaggctctg ataataatta
     1561 gcactaaaac tatttaaaag ttctattcct atacctagtg ctaaatatat aaaaactaat
     1621 aacctctctt tttttgctat ttctattaaa tatttcataa ctgtacacct atccttattt
     1681 attttacttt attaacttaa aataatttta tagataaaat gtttctttat ttgtttaaat
     1741 ttagtgtgat tattaatcat ttcaagttaa caaaacctaa agtttttagc agtacagttc
     1801 tatttgttag caaattatta ttttactcag tacattatgc taacatataa tctaactgta
     1861 ctgagtaaat atctcgtttc atcatataaa atctcctttt tatagtttaa tatatatagt
     1921 taaatttact atataatctg aatatttatt tttctatcac ctttaccaga aataaaatca
     1981 attatttatt gtataagcaa aacttcataa tattttcaac atataattat tatacagaac
     2041 tcctaagtta atttaaagtt agaatttatt tttataagaa atgtcattga cctaccttta
     2101 ataaaacagc caccattttt ttatgtatta acatataata tatagtaatg aaaacatatt
     2161 aattaaaaaa tattgtataa attagtttat ctgtaacaaa tccagtttat gtttcaacaa
     2221 gcacgtaaga cttcttgaaa tttaatctgc ttcctttaaa tatctattac ttgttttaag
     2281 cataagttta caatcttcta taacatttat catttcttca gatttctctg acgtacttaa
     2341 aatacaagtt gttacattgt ttttctattt aattagtaat gtatcatgta aaaaagaagc
     2401 ataaatgagt ttactttata caaacttcat ttatgctcca ctcatatacc tatcatagct
     2461 aatagaaact attactcatc ttgaatctat ttcatattta gatatttata tctttcttat
     2521 tttcttaaat caactctagc tttaaacatt aaacccctta aatcttcaca aaatctcttc
     2581 ctatacagac ttactctttg ccttacatat catctgagtc atagtaccca tagggcaata
     2641 aacacaccat gaacgaggtt taaacaatat catagtaatc aaaccaagaa cagttgatgt
     2701 aagcataaca ctataaaaac caaatgcaaa ctgtaccacc caaggtgaaa ctaaagttcc
     2761 atgatacgcc cattgccaag gcatttttat agtccacaac aaagtcacta cttctttaaa
     2821 atttctactt ccttcaaaca ctaaataagt tgaaaaaagc atattggcaa acattaccat
     2881 aaaaaatgtc aaaaacccat atctaaacca cttacttcta ataaatttag gaatatctct
     2941 atttcttgac agcttcaatt tatttccaag caaattaaac agttgccctc ttccacaata
     3001 cttattacag taagctttat ttcccttaac aatagaaata gctagtggaa caaagaaaca
     3061 aattagccca atccaagcga aaataatatt tacaaatccc aataccaaat atattgaaga
     3121 aaaaatccat aaatagtcat accaatgttt tttattattc atgacactta ccttcttcct
     3181 cttttaatat tgttataatc tcaattacag atgctggaca tatcttttca caaatcccac
     3241 atccaacaca taaatctcta ttaatttttg cactaatgcc acatggaact gagatagcac
     3301 ttcttggaca tgcttttata cagctaccac aagcaacgca ctcatttgct aaaatatttg
     3361 catacttctt ttttttatta gacataataa cctccctata ttattaatct ataagaatta
     3421 tatcaagata cttacttttt atctgtgact taatcacata aaacctcaat attaaaaatc
     3481 acactctaaa tacaaaggag tttcaaataa aaaacgatat gaagtcatta agtttagctt
     3541 catatcgcct tttcttttgt gtatctatat aaatatttat tatctaatat ttaatatatc
     3601 tataatattt tcttatacag ctctataatt ttacacctaa tcatattcta ttagtaacag
     3661 gttaataccc atctaaaact ataactttat tcacaatctt attctattag ccacaagtta
     3721 aactcatatt gaagtttatc tttagttaca ctcttactct attaatcaca agttaagccc
     3781 atattgaagt ttatctctat ttacactctt actctattaa tcacaagtta agctcatatt
     3841 gaagtttatc tctatttaca ctcttactct attaaccaca agttaaactc atattgaagt
     3901 ttatctctat ttacactctt actctattaa tcacaagtta agctcatatt gaagtttatc
     3961 tctatttaca ttcttactct attaaccaca agttaaaccc atattgaagt ttatctctat
     4021 ttacactctt actctattaa ccacaagtta agcccatatt gaagtttatc tctatttaca
     4081 ttcttactct atcaatcata ggttaagctc atattgaagt ttatctttag ttgcacctta
     4141 ctctattaat cacaagttaa acccatattg aagtttatct ttagttacac cttactctat
     4201 taaccacaag ttaaacccac attgaagttt atctctattt acactcttac tctattaatc
     4261 acaggttaaa cctattataa tcttaacttt attcacaatt ttattctatt agccacaggt
     4321 taaatctatt ataatcttaa ctttattcac atattacatc attaatcgca tatcaagatt
     4381 ttataataag ctttaactaa acacacgatt ttaccttatt cgtatagttt aatctattaa
     4441 attattatat aatcttaagc aaacatacaa ctctacacta ttaattatag cccctccttt
     4501 taaatactca taatcttata cacaacctta ccttattaat cacacattta actcatatat
     4561 atcatcaatt ttattcacaa aatcatgctg ttaatcacac ttatactcct ctataatctt
     4621 aaacttatac acaaattagc cacaatttta tttcaactat aataacttct atttcagaag
     4681 tatatcacac ttgtattctc caaaaatatc tatttttttc taacaatatg cctgacatta
     4741 ataacactca tttcaaacat cctcgctaat tccatataat ttttcccatt aaacaattta
     4801 atcataagct catttctagc ctcttttaat gccccttcca gtgttttaat ataaaaatat
     4861 gttcccccac tttgttgaca caattcaagg tattccaata aactatactt atgtaactcc
     4921 tttggaatac ttacttctat cttgtgttct tcaagtaatt ctataatttt cgttaattcc
     4981 aacatatttt cacctcatat tttataatct atttgctatt tcctgacttt taataagctt
     5041 gttattacta ctttattgac ctaataattt agttcttgaa aatgtaactt atccccaaag
     5101 taacaaaaat gcttatgcct agtctgtcca tctctacact tcgcaactat taaatcaacc
     5161 atcttaattc ccctgtattc tgatgctcta actgcctcct cactttctcc aatatctctc
     5221 actgcttctt cataatcact cccctttaat tgatgtatat aaaccacatt gttgctatcg
     5281 tggtagatgg ctttgctatc cctcataggt ctatctccat aaggtcttga atctttcatt
     5341 tcatcattta actgacttaa ttggattaca ggtatattaa aatctaaagt catattttta
     5401 agttctctcg aaaaagtagc tacctccctt tctctcttat ccatgctttt ctgagatgta
     5461 aggagttgca cataatccac tattaataaa tctggcttta cctgccttat cctctttcta
     5521 attgcactaa ttgtatctat cttatcatta atataaatta agttattttc gcttaaatca
     5581 cctatagcat ttacaataag tttccaatca ttttcatcta tttctttaga tttcatattc
     5641 cttgtactta caccagttac tctgcatata tttctcataa atacttgctc actggtcatt
     5701 tctctactta taaataaagt ttttttacct tgcttaaatg aatttaacat tatttgaagt
     5761 gccaaagcag ttttcccaac ccccgattta gctgctattg tggttagctc tcctttaaaa
     5821 agacctccta tagtagtatc aagtaatttt ataccaaatt taaatccaat atcttttttg
     5881 ttttctaaaa aatcaagcac cttccctgct atactattta catcatcatt ttcataagta
     5941 tccttgtcca aaatctcctt tatattactt tcaaatttgt aaatacaagt atcgatattt
     6001 tctccaaggt ttaggctgtg taaaaggttt tggcagtttt gatttatgct tcttctagct
     6061 aaattttctt ttaaaatttt aatatgacca tcaagaccaa aattctgacc ccaagtaatt
     6121 agattagtca catcactcgt ttttattgct agagattttc tatctatctt tgatttcaca
     6181 gtggccacat caatcactat gttttctttt ttaagttctt tcataatttt aaatatctca
     6241 agacatatat cattcataaa catgttttca tttaattcat ctagcttata gcttaattta
     6301 ttgtctaaaa gaattgtgcc taaaatagac ttctctattt caacatcttt tgatttaccc
     6361 atataaatta atctctcttt ccttataagt ttttatattt tgatttttaa atacattatc
     6421 aattataaat acattatttg ttatgactca ctatttgtta tgactatatt attcgtcatg
     6481 atatactaca aattattaat atactattta ttctgataca tataatttac tacataatag
     6541 caatatctta tttgatataa cttactacca attattaata cattgttcaa tataattcat
     6601 tactcgatag taatatctta tttgatataa cttactgcca attattgata tatcgttcaa
     6661 tataattcac tacctgatag taatatctta ctt
//
LOCUS       DAELXX010000155         6643 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.157, whole genome shotgun sequence.
ACCESSION   DAELXX010000155 DAELXX010000000
VERSION     DAELXX010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.157"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            297..1241
                     /locus_tag="KPH29_003379"
     CDS             297..1241
                     /locus_tag="KPH29_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HBG1468944.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            1263..1889
                     /locus_tag="KPH29_003380"
     CDS             1263..1889
                     /locus_tag="KPH29_003380"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBG1468945.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            1923..2972
                     /gene="hisC"
                     /locus_tag="KPH29_003381"
     CDS             1923..2972
                     /gene="hisC"
                     /locus_tag="KPH29_003381"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBG1468946.1"
                     /translation="MREKESIRELRGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYE
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            2975..3553
                     /gene="hisB"
                     /locus_tag="KPH29_003382"
     CDS             2975..3553
                     /gene="hisB"
                     /locus_tag="KPH29_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HBG1468947.1"
                     /translation="MRIWKVERNTFETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            3555..4160
                     /gene="hisH"
                     /locus_tag="KPH29_003383"
     CDS             3555..4160
                     /gene="hisH"
                     /locus_tag="KPH29_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBG1468948.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLGLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDITLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELVAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            4157..4879
                     /gene="hisA"
                     /locus_tag="KPH29_003384"
     CDS             4157..4879
                     /gene="hisA"
                     /locus_tag="KPH29_003384"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBG1468949.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGINTKIIEDIANNIDIPIQVGGGVRDKGKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSDVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            4861..5619
                     /gene="hisF"
                     /locus_tag="KPH29_003385"
     CDS             4861..5619
                     /gene="hisF"
                     /locus_tag="KPH29_003385"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBG1468950.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKNMGVEVRL"
     gene            5647..6315
                     /locus_tag="KPH29_003386"
     CDS             5647..6315
                     /locus_tag="KPH29_003386"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBG1468951.1"
                     /translation="MDNKCNNVYSDEVEEFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDAIKKTLKDKVACYFSRSRQKLWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMEPEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
BASE COUNT         2727 a          551 c         1282 g         2083 t
ORIGIN      
        1 aactgtaaat ttagaatatt aataaaaatt atttgggagg aaattatcat ggtgagatta
       61 aatatagtaa aattaaatac aaaattaaat catcaccatc atcatagatt aaagggattg
      121 taatatgaaa ttaattcata gacacaagcc ctgtttttag tgtaattggt cttgtatcta
      181 tgatgggaag taattttagg agaaaattat agccatatag gtataaagcc tatatggctt
      241 ttttattgtt ttaaatttgc aaatataaga gtaaatttaa tgaagagggt gagaatgtga
      301 aatttttgag gattgaagat gaagttattt attctaaaaa aagatataaa ttaaaaaggg
      361 aaatagataa actttttttg gatgaagaat attttcaaat agaaccacaa ttatttgaag
      421 aatatgatga atttacaact ataaataata aaattccaga agaatctatg gtaaaggttg
      481 taaatggaaa agtaatggta cttagagctg atataactac aagtataata aaaagccttg
      541 taccaagatg ggaagatggt ttaaagctta agctttttta taattcatca atatataaaa
      601 ataaaaatac agttggaata aaagagataa gacaaatagg ttgcgagtat ctaggagaag
      661 cctctgtaga agcagataga gaagtagtga aattagctct taagatactt gaaaaataca
      721 ataataattt tattttagag gttgggagta gcaagtatat tcatggtttg ttagaagaat
      781 taaatttaaa taagaattgt gaaaatcaaa ttaaaaattt actatatacg aagaacactc
      841 atgaattaaa agtatatatt gaagaactga aaattaagcg tgaagtaaaa gagctattat
      901 caaatatttt aagcttgcaa gggaatttat acaacgttat tgaaaaatca aacaagttct
      961 attgtaataa taagatgaag caagctcttg aagaattaaa gcaagtaaat aatttaattg
     1021 aggaatgtaa ttttttagat aaggctagat ttgatttatc aatgattact atgcttgact
     1081 attatgaagg aattatgttt agaggatact acccaaactc atataaagaa attcttagtg
     1141 gtggaagata tgattcttta actaaagagt atggaaaaga aattccagcc attggattta
     1201 cactaagtgt tgatgagtta atgaagtatg tatataagta aagtttagaa ggagggattg
     1261 ctttggatta tttgactata gcactagcaa aaggacgaat tgaaggggaa tcatttaaaa
     1321 aatttaaaaa aatgggtcta ggagatagta tagacacaga tacgagaaaa ctaattttta
     1381 aagatgaaga aaataaaatt atatatatac atgtaaagcc atcagatgta gttacatatg
     1441 ttgaaaaagg agtcgctgac cttggtatag caggtaaaga tactatttta gagaatgaga
     1501 cagatgtata tgaaatatat gatttaggtt ttggaaaatg taaatttgct gtagcaggat
     1561 taaagggaga tagcatatat agagaagatg aatatctaaa agtggcaaca aagtatccca
     1621 atatagcaaa aaagtatttt aaagagaaag gtcaaaaaat tgaaataata aaattaaatg
     1681 gttctgtaga actagctcca atagtagggt tgtcagatgt aattgtagat atagttgaga
     1741 ctggcaatac acttaaagca aatggtcttg aaatattaga agacatatgt aatataagtg
     1801 ctagaattat atcaaataga gctagctata gatttaaata tgaacaaatt cagaatataa
     1861 ttcgtttatt tgaagaactt gataattaaa atataattga actttataag gagggattta
     1921 agttgagaga aaaagaaagt ataagagagt taaggggata tgaaccaaat cacgtaaact
     1981 gtaaagtaaa acttgatgca aatgaaggaa gtaagaggtt gtttaaatac cttattaaag
     2041 aaatttcaga tagtgatata gatttaaatt tatatcctga agattcttat agtgatttga
     2101 aagaatctat aatagattac ataaatattt ctggagtaaa taagaaaaat cttctagtag
     2161 gaaacggttc aagtgaaata attgatttaa taatacatac ttttgtagat aaagatgaag
     2221 taatattgag tttctctcca agttttagta tgtattctat atatagtcag ataaatgggt
     2281 ctaaatttat aggtgttgaa agtgatgaaa atttagttat aaatatagat aatgtgattg
     2341 aaaaagtaaa ggagaataat cctaaaatag taattgtatg taatcctaat aatccaactg
     2401 gaacaatatt aaagagggaa gagataataa agcttttaga ttcaactaat agtctagtgg
     2461 ttttagatga agcatatatg gattttggag aagaaagcat gttaagtgat gtttttaagt
     2521 atgataatct aatagttctt agaactttat caaaggcatt tggattggct ggaataagaa
     2581 caggatatat gttatctaat agtagtttaa taaattctgt tgagaaagta agacctccat
     2641 ataatttaaa ttcattatca gactttattg ctacaagagc acttagaaat aaagatgtgg
     2701 taaaggctta tataaaggaa gtaaaagagg aaagagaagt tttatatgaa gaaatgattg
     2761 gtatgggaat taaagcatac aaatcacaag caaattttat attattttat tctgaaatcg
     2821 aaaatttatc acaaaaactt attgatagag gagttttaat aagaaaattt ggtggaaagt
     2881 tagaaaatta ttacagagtt acaattggtg ataaagaaga aaattctatg tttgttggag
     2941 ctataagaga tattttgaaa aaggagaaat aggtatgaga atttggaagg tagaaagaaa
     3001 tacttttgaa acacagattt tagtagaatt aaatattgat ggttctggta aagctgaaat
     3061 agatactgga ataggatttt tagaccatat gcttacctta atgtcatttc atggcaagtt
     3121 cgatttaaaa gtgatttgta agggtgatac atatgtagat gaccaccata gtgtagaaga
     3181 tataggtata gctataggtg aagcatttaa aaatgcacta ggtgacaaaa aaggaatacg
     3241 aagatattct aatatctata tacctatgga tgaatctcta tcaatggtag caatagatat
     3301 aagtaataga ccttatcttg tatttaatgc taaatttgat acccaaatga ttggtagtat
     3361 gagtactcaa tgctttaaag aattttttag agcttttgta aatgaatcga gagtaacatt
     3421 acatatcaat ctcttatatg gggaaaatga ccatcataag atagaaagta tttttaaagc
     3481 atttgcaaga gctttaaaag agggtagtga aatagtttct aatgagatag catcttcaaa
     3541 gggggttttg tagaatgaat ataatagtag actatggtct tggaaatata gattctgtgt
     3601 ctaggggttt tagaaaagct ggaatagaaa ctaaaatttc tagtgatata gatgaaataa
     3661 aacaggcaga ttcacttata cttccaggtg ttggagcttt tagagattct ataagtgcct
     3721 tagataaact aggattgata ccaataataa aggagcatgt ttctaagggt aaatttatga
     3781 taggtatatg tctaggaatg caattacttt atgaaaaaag ttatgagtat ggagaatatg
     3841 aaggattagg acttataaaa ggaagtattg ataaactgga tataacttta aaagtgccac
     3901 atatgggatg gaataatcta aaatttaata aggcaaatga tgatatactt aaatatataa
     3961 acgaagatga ttatgtatac ttcgttcact catattatgc aaattcatca aatgaagaac
     4021 tagttgcatt ttcagaatat gaaaagaaaa tccctgctat tgttagaaaa ggcaatgtat
     4081 atggtataca atttcatcct gaaaaaagtg gagaagtagg gttaaatata ttaagagcat
     4141 atggggagat gataaaatga taatatttcc agcaatagat ataaaagata ataagtgtgt
     4201 aagactcaca caaggagaat ttgacaaagt aaatgtatat tatgataatc cattagaagt
     4261 agcatataaa tggaaaaatg aaggagcaga atatatacat atagtagatt taaatggtgc
     4321 tagaagtgaa tttggtataa atacaaaaat aattgaggat atagcaaaca atatagatat
     4381 acctattcaa gttggtggtg gagttagaga taagggaaaa gtcaaaagtt taataaatgc
     4441 tggtgttact agagtaatat taggtagtat agcgattgaa aatttgaatt tggttgaaga
     4501 attggtcaat gaatataaag agaaaattgt agtttctata gacgctaaag atggaaaagt
     4561 agcagttagg ggatgggaag ttgtaagcga tgtagactca ttaacattat gtaaacaact
     4621 tgaaaagata ggtgtacaaa ctattgttta tacagatata tcaaaagatg ggatgttgca
     4681 agggccaaat tttgacatat atgaaagaat agcaaaggaa acgtcattaa atgttattgc
     4741 ttctggaggc gtaacttcta tagaagacgt aaaaagacta aaagctatga acttatatgg
     4801 tgcaataata ggtaaagcgc tttatgataa aaagatagac tttaaggagg cacaacaatt
     4861 atgcttacta ggagaataat accttgtcta gatgtgagaa atggaagagt tgtaaaggga
     4921 aaaaaattta aagatatagt agatgttgat agtccagaag tacttggaaa attttatagt
     4981 gattgtggtg cagatgaact tgtattctat gatataactg catcaaatga agagagaaag
     5041 acatctttag aatttgtgac aaaagttgca gaaaatataa atataccatt ttgtgttggt
     5101 ggaggagtaa ataaattaga agatttcact gatattttaa gaaaaggtgc tgataaggtt
     5161 tctataaatt catcagcagt caaaaatcct gaattaataa gggaagcttc attaaaattt
     5221 ggagcacaat gtgttgtttt atcgatagat gcaaagaaaa atgaagaggg ttcatggagc
     5281 gtatacgtaa aaggtggaag agaaaaaact aatctggatg caattgaatg ggctgttaaa
     5341 ggtgtagaac ttggagcagg agaaattgtt gtaaatagta tggacgaaga tggaatgaaa
     5401 aatggatatg atatagaatt attatcaaag ataacttctt tagtaaatgt acctgtcata
     5461 gcatctggag gcgctggaaa aaaggaagat ttttatgagg cagttaataa atctaatgta
     5521 gatggaattt tagcagcatc tgtttttcat tttggcgaga taaaaataaa cgatttaaag
     5581 aaatatttaa aaaacatggg tgtagaagta agattataag ttaaatacta aaaggagatt
     5641 agaaatatgg ataataagtg taacaatgta tacagtgatg aagtagagga atttataaga
     5701 agtataaagt ttgatgataa agggttagtc cctgttgttg ttcaagaggt agttagtaaa
     5761 gatgtactga tgttagcata tatgaataaa gatgctataa agaaaacctt aaaagataaa
     5821 gtagcttgct attttagtag aagtagacaa aaactttggg ttaaaggtga gacatctggt
     5881 aatactcaaa aagtagttaa gatgtcttat gattgtgatg tagatactat actacttttt
     5941 gttgaacaaa ctggtgtagc ttgtcataca ggcaattatt cttgttttta cagagacttg
     6001 tttgatgata gttcaaaaat ggagcctgaa gtacaaacaa atatattgaa agaattatat
     6061 gatttaatca atgaaagaaa aaataatcca gttgaaggtt cttatactaa ttatcttttt
     6121 gaaaaaggaa tagataagat tcttaaaaaa gtaggtgaag agagtagtga ggtaattata
     6181 gcatcaaaaa ataccgataa aagtgaactt atttacgaaa ttagcgattt agtgtatcat
     6241 acattggttt taatgataga aaaaggtgtt gaaattgatg aaataaaaaa agaattactt
     6301 aaaagaagaa aatagaatag gatagattaa tggtaataag aaaatatacc gcttttaata
     6361 aaaagtggta tattttttat tttatatttt cgatttatag aataatattt aaattacttg
     6421 ataaaattaa ctatatatat aatttattga taaaataatt gtagtacatt atttatttta
     6481 gaaaaaagat aattatctaa ttatatttaa ggataataat atgaaaataa tttttatatt
     6541 aaaaaatatg aatgaaaata gtaggtttta taaaaaacat atttagattt taacatctaa
     6601 aaggaatttt caaacctaat atttcatttt taaataaaaa aat
//
LOCUS       DAELXX010000156         6615 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.158, whole genome shotgun sequence.
ACCESSION   DAELXX010000156 DAELXX010000000
VERSION     DAELXX010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6615
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.158"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(29..637)
                     /locus_tag="KPH29_003387"
     CDS             complement(29..637)
                     /locus_tag="KPH29_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBG1468952.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(746..3484)
                     /locus_tag="KPH29_003388"
     CDS             complement(746..3484)
                     /locus_tag="KPH29_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HBG1468953.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNINLYTVSINMYRIILLW
                     IFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYFIRNIFSDINWNMIIILINICISMFNLLY
                     LLNKLDSNIEHYKLIKDLTYFVFFTTIICFVAISKINIVDSDRLIGVIYLLLCNHTYY
                     VYVYSLNNRVVYPYYELDSINKKLSKKSEQLGKINLAIEKDMIIQRTLKNYIDQRKEL
                     LRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMNNYYKILTFIKEDEKVAEKLKK
                     FDNDISIIDKLVELNDKIYLLSLSNNKDESNYLISLNDITNEIKIDEEIRNINKDYEN
                     IILNIPSPILVRSAEGGIKKNKVISINKKYEKSFKCVSSDLVDMTLEQYFETFNIDFF
                     DNRKFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEHVEEVRVGDYWSDNKLFK
                     LLTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLSTSRVLYTNRAFRELFGI
                     SSDVLGVTTQKYRNILVKKYINNLNIGEREKSIHIVNENNHIKEVKMISRTLYFGQKR
                     SRVRIIKDLSVQRESERLKKALIKQRQYDQMKMEFYANISHELKTPLNNIYSSVQLIE
                     NLYKKGKIIDFQDILKEHIKITKQNMFRLLRLIDNIINISQVKSDIYKIKAVNFDIID
                     ITERIVTSISSYAKSKGIDLIFDTDEETVMVGLDPESIERIILNILSNAIKFTLEGGE
                     ILVGIYKKDETVEIIIKDTGVGIDKEKLNDIFNRFKQIENSGISNEFGSGIGLCLTKS
                     LIEIQNGKIYIDSKVGEGTNVKVIFPIKEVVEEVYDNSNYNDNIEKFEIEFFDIYK"
     gene            complement(3632..4423)
                     /locus_tag="KPH29_003389"
     CDS             complement(3632..4423)
                     /locus_tag="KPH29_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG1468954.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(4459..5115)
                     /locus_tag="KPH29_003390"
     CDS             complement(4459..5115)
                     /locus_tag="KPH29_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1468955.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(5108..6073)
                     /locus_tag="KPH29_003391"
     CDS             complement(5108..6073)
                     /locus_tag="KPH29_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HBG1468956.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKKLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEHVKQYLTSKGIRWEEMINE"
     regulatory      complement(6179..6285)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
BASE COUNT         2104 a         1109 c          603 g         2799 t
ORIGIN      
        1 tagatacata tcttacctat ataaaaactt attctatata aaaacgccat aaaaaatctt
       61 ttgcttcttc tgcataatct atatttatac gtttactagt ttttatatct aactcatctt
      121 tactaaatac tttttttcct ttatcatcat aataaaaatc acttatatat aattcttcac
      181 ccattatact cttactattt agacttctat ctatttttaa agctttgcaa agttttcctg
      241 gtccgttagt aatatttttt acttgatatt tactcaactc agtgtaagct ttgttgtatc
      301 tattcataga tatctcatct aaagaagtta taggctctac ccctcttatt aatacacact
      361 ctggcacatt ttctatattt gcagataaat ttaagcaatt atacatacca taaattaaat
      421 aaacatatat atgtccacca tctaaatata atggcttagt tctatctgtc tttttattgc
      481 catatacatg tgctccttta tcatttacac ccatgtatgc ttctgtctct atgattttgg
      541 taacaattac tttattttca tatttcctta ttagatattt acctaatatt gatttggcta
      601 aatcaattcc attttttcta aaaaaatcct tttccaaaat ttcacctcat aaaatttatt
      661 taaaacgcat caaatacaac taaagtaata aataaaatta actcttatta atgtgatata
      721 tataaacaat aaatttagcc atttattatt tatatatatc aaaaaattct atctcaaact
      781 tttcaatatt atcattgtaa tttgaattat catatacttc ttcaacaacc tctttaattg
      841 ggaatataac ttttacatta gttccttctc ctaccttact atctatgtaa atcttcccat
      901 tttgaatttc aatcaatgat tttgtaaggc ataaacctat accacttcca aattcatttg
      961 atataccact gttttcaatt tgtttaaatc gattaaatat atcatttaat ttttctttgt
     1021 ctatgcccac tccagtatct tttataatta tctctactgt ttcgtctttt ttatatatac
     1081 ctacaaggat ttctcctcct tctaaagtaa attttatagc atttgataat atatttagta
     1141 ttattctttc tatagattct gggtcaagcc ctaccataac agtttcttca tccgtatcaa
     1201 aaattaaatc tattccttta gacttagcat aactactaat agatgtaact attctttctg
     1261 ttatgtcaat aatatcaaaa ttaactgctt taatcttata tatgtctgac ttaacttgag
     1321 aaatatttat tatattatct attaatctca ataatctaaa catattttgt ttagttattt
     1381 ttatatgttc ttttagtata tcctgaaaat caattatttt tcctttttta taaaggtttt
     1441 caataagttg aactgaacta tatatattat ttaaaggtgt cttaagttca tgagatatat
     1501 tagcataaaa ctccatcttc atttggtcat actgtctttg ttttattaat gcttttttaa
     1561 gtctttcaga ctctctttgc acacttaaat ctttaataat tctcactcta cttcttttct
     1621 gtccaaagta taatgttctt gatatcattt ttacttcttt aatgtgatta ttttcattta
     1681 ctatatggat actcttctct ctttctccta tatttaaatt gtttatgtat ttttttacta
     1741 atatatttct atatttttgt gttgtaactc ctaatacatc actagaaatt ccgaacaact
     1801 ctctaaaggc tctatttgta tatagtactc ttgatgtact taaatcctca agaaaaatag
     1861 cctctggtat aacatcaagt aatttttcat atattatttt ttgttcattt actgttctaa
     1921 gaatatttat ctctttagtt atatctctaa atgtaagcaa tttaaagagt ttattatctg
     1981 accaatagtc tccaactcta acttcttcaa catgttcttc accttctgag tcagacatca
     2041 caaaattaac tatacagttt gctgtgtcat tgtaacttac tatttcagct ttcttttctt
     2101 gtgttagatt taatctttta aatttcctat tgtcaaagaa atctatgtta aaagtttcaa
     2161 aatattgttc taatgtcata tcgactaaat cacttgaaac acatttaaaa ctcttttcat
     2221 attttttatt tatacttatt accttattct tctttatgcc accttcagca cttcttacaa
     2281 gtataggaga tggaatattt agtataatgt tttcatagtc tttatttata tttcttattt
     2341 cttcatctat tttgatttca tttgtaatat catttaaact tattaagtaa ttgctttcat
     2401 ctttattatt acttaaactt aacaaataaa ttttatcatt taactctacc agtttatcaa
     2461 ttatacttat atcgttatca aatttcttca atttttcagc aactttttca tcttccttta
     2521 taaaagttaa tattttataa taattattca tatcctttga aaattcatct ttaaatttat
     2581 tatttgtgta acttatatta aaatcatagt ctgttactat ccatacattt ggtattgtat
     2641 ccagagcttg tctaagtaat tcttttctct ggtctatgta gttttttaat gttctttgta
     2701 taatcatatc cttttctatt gctaaattta tctttcctaa ttgctctgac tttttactta
     2761 attttttatt gattgaatca agttcataat atggatacac tactctatta ttaagactgt
     2821 atacatatac ataataagta tggttacata atagtaaata tattactcct attaatctat
     2881 ctgaatctac tatattaatc ttagaaattg ctacaaaaca tataatagtt gtaaaaaaaa
     2941 caaaatatgt taaatctttt attaacttat agtgctcaat gttactatca agtttattca
     3001 acaaatatag taaattaaac atagatatac aaatattaat taatataata atcatattcc
     3061 agttaatatc actaaaaata tttcttataa agtaatctaa tatcacaaaa gcactataaa
     3121 aaattagtat tgggcttatt cttagttttt ttactttaga aatggttaag tatataaaag
     3181 tagacatatt aattatgctt aagttaagca atacaatttg gaatttttga atttttaaat
     3241 ttactactcc taaaatactt acaaaaacta atacaagaca ccctactttc aatatattta
     3301 taaattcatc tgttttaaag ctacttatat aaactataga atatccaaat atccaaagta
     3361 aaattattct atacatattt atagaaactg tatacaaatt tatatttaaa agtgcaataa
     3421 ccaaaaataa cccattagct agtatataga ccttattctt acttaaagtt cctttagatt
     3481 ccatattccc caccacttct tcatttttat atagttttac ctataaatat gttaccacaa
     3541 ttcttccaaa aaatttgtca ctttctagtt ataaaaaaaa atgagtaaat atttttaatt
     3601 ttactcattt tgtagatttt gtatattcag tttaaaaagc tggaactata cttcctttat
     3661 attcatcttg tataaatttt ttaacttctt tactattcat agcctctgat aaagccttaa
     3721 tcttatcact atctttatta ttttctctac aagctattat atttacatat ggagaatcac
     3781 ttgattctat tactattgca tccttagtag gatttaagtt agctgttaaa gcataattag
     3841 aatttattac tgctccgtca acatctttta atactgttgg taattgctct gcattcattt
     3901 cttttattac tatattttta ggatttttag ttatatcttt tttagttata agctctccat
     3961 cttttacttc tatcaattta ttttttgcta ataatttaag tgctctagct ccattagttg
     4021 catcatttgg cacagctata acagctccat cttttaattc atctaatgac ttaacttttt
     4081 ctgaatagaa tcccattggc tctatatgaa ctttagatgt ataagtaagt ttatatcctt
     4141 tctccttcac agtttcctct aaaaatggaa tatgttgaaa gaagttagca tccaaagacc
     4201 cttcatctaa agctgtattt ggaagtacat aatcatcaaa tatttttact tctaaatcat
     4261 atcctttttc ttttaacaaa ggttttgcaa cttccaatat ctttgcatgt ggatttgatg
     4321 aagctcctac tagtattttt ttatcctctt tgtttgaaca acctactgct gatattgcta
     4381 ttgctgacac caatgctact gataataatt tctttaattt catttttact ccccctcttt
     4441 aaaatgttta taattaattt attttagttt cttataagcc agatttccta ctccctggat
     4501 tatctgaact aatattataa gtgctattac agtatatacc attactgctg tatcaaatct
     4561 ttgatatcca taaataagtg ctatattacc taatcctcca gctcctactg cacctgccat
     4621 agctgtataa ccaagtatag atattattga taatgtaatt ccagatacta ttgaaggcat
     4681 agcttccttt atcataactt taaatactat ttgtcttttt gtagctccaa aagattttgc
     4741 tgcttctatc aatcctttat caacttcatt taatgagctt tctataattc tagcgataaa
     4801 cggtgctgcc ccaatagtta ttggcactat tgcagcagtt tcccctatac ttgttccaac
     4861 gattaatcta gttattggaa tcattgcaac tataagtatc ataaatggga aacttctaaa
     4921 tatatttact ataaatccta atgcactatt tattgtgcta tttggtttta atccatctgg
     4981 ttttgtaact actaatatga ttgctaatat aaatcctaat attgttgcca ctatagttgg
     5041 tactataacc atatacaaag tttgtaacaa tgcatttgga aatagtgttg ttaaaaagtc
     5101 aattaaacta ttcatttatc atttcctccc atcttatgcc cttactagtt agatattgtt
     5161 ttacgtgctc tccactttta tctgaaatgt ttataataag tgaacctaaa atatcatctt
     5221 taaattgttc taatttccca aatattatag acacatctat atttagttct cttgccatag
     5281 tcgtaataat agcttcatta gaaatatctt tagggaatag tatctttata tttgttcctt
     5341 ttggcaatat tatactttct tcacctatta attttctaag tcctttagta tttcttaaga
     5401 atatttcttc tgtatctcct acctctaata cttctccatt ttccattatt gctactcttc
     5461 cacaaatctg ctttataact tccatttgat gagttacaac tattattgtt attcccaatt
     5521 ttttatttat atcttcaagc aatgaaagaa ttgactttgt agtatttggg tctaatgctg
     5581 aagttgcttc atcacaaaga agtacttgtg ggtttagtgc taaagctcta gcaattgcaa
     5641 ccctttgttt ttgtccgcca cttagatttc tgggtttatc atttttcttt tcagatattc
     5701 caactacttc taataactct aaaactcttt ttttaatttc tgcctttgag tatccaaaac
     5761 attctaatgg caatgctaca ttatcaaata cagtttttct ttcaagcaat gaaaagtgtt
     5821 gaaaaatcat tcctaactct tttcttaaat ctctcatttg cttttcattc aatgacttta
     5881 cttctttatc agaaacaagt acactgcctt cttggtattc ttctaatccg tttatacatc
     5941 tcagtagtgt tgatttccct gcaccactat gacctattat tccaaaaatt tctccagatt
     6001 ctatttcaat acttacatct ttaagcactt gaatctttcc ataatactta ttaacatttt
     6061 ttatgctaat cataaaattt cctcccatat attctgctca gtttttatgt ttatcctctc
     6121 ttatgtatta caacaaaata aaagcccgag tataaactca ggcttagaaa gctatttata
     6181 ctcatctatc aactatatta gttgcaggat ttagcaccaa tgtatataaa atatactggt
     6241 tgccgggttt caaagggcca gtccctccac cactcttgat aagacgttat ttaattcaat
     6301 tctattatca tttgttgcta tgttatatat gatatattca ttaatagtat ttgtcaataa
     6361 atttatttaa attttttatt ttttattatc gttaattaac tatttttaat tagttttcta
     6421 gctcctaaca ctttatattt taatgtctat ataactatag aacatcatta taagaaataa
     6481 attaaataac atcaatctat ctaaatcatc tgtaaagtaa attaagaaaa ttaacatttt
     6541 atccaaactc tatcctacta ttaaataaaa taatttaaat acaatgatga ttatatagct
     6601 ctaatacttt agact
//
LOCUS       DAELXX010000157         6610 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.159, whole genome shotgun sequence.
ACCESSION   DAELXX010000157 DAELXX010000000
VERSION     DAELXX010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6610
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.159"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            192..500
                     /locus_tag="KPH29_003392"
     CDS             192..500
                     /locus_tag="KPH29_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468957.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(662..2452)
                     /locus_tag="KPH29_003393"
     CDS             complement(662..2452)
                     /locus_tag="KPH29_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1468958.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(2452..4179)
                     /locus_tag="KPH29_003394"
     CDS             complement(2452..4179)
                     /locus_tag="KPH29_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1468959.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSARVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            complement(4424..4988)
                     /locus_tag="KPH29_003395"
                     /pseudo
     CDS             complement(4424..4988)
                     /locus_tag="KPH29_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
     gene            5277..6389
                     /locus_tag="KPH29_003396"
     CDS             5277..6389
                     /locus_tag="KPH29_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBG1468960.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     AEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
BASE COUNT         2270 a         1045 c          846 g         2449 t
ORIGIN      
        1 tattttactt ttgtttaacc taaagataat ttacttttaa tctatatgaa ttatcatata
       61 aataatcaga aagttatctt tataaattat agttttagat ttataattta taaaatattt
      121 atgttctttg taaaattttt tagaagcttc taatgttatg ctataaaact atcttgagaa
      181 gggctgattg aatgtttaat aaaatatatg aaaagaaaga atttatagtt tttcaagtta
      241 aaaaaggtta tgtagtttat aatactagaa agagttttga agaaggtcat acacatttaa
      301 agcattttga agcagcaaag acagctattg atttagcaat aaataaaaag attcctagaa
      361 gcaaagatgg atattatctg actagtttaa ttcgcataag tgatgatggt tattacattg
      421 ataagttaag cgaattgtta tatgttagag aacaaaaagg taaaaaagaa aagtactgta
      481 attctggtta tcagatgtaa caagactagt tgtgaattat aagtaccaat cataaatacc
      541 tttagtctta aaatcaaaga gtatcagtaa atctataaaa aaacaactta ctgatactct
      601 ttttaattat atgattaata tttgaatttg atacaaaatt ttatacaaaa gtttatgtta
      661 tctattctga ttctttgtct gcaaattggc tattataaag cttctcataa aatccaccct
      721 tttccattaa ttcatcatga ttcccctgct ctataatact tccattattc ataactaaaa
      781 tcaagtcagc attgcgaatt gtagaaagtc tatgtgctat aacaaaacta gttctaccat
      841 tcataagatt tctcattgct ttttgaagca taagttccag cctagtatca acagaactag
      901 tagcttcatc aagtatcaat attgaagggt ctgatataaa agctctagct atggttaaaa
      961 gctgtttttc ccctagagat atattagatg cttcttcatt taagaacata ttgtatccat
     1021 ctggaagagt ggttataaag tgatgaacat ttgctacttt tgctgcttgt ataatttctt
     1081 ctttagtagc tccaaatcta ccatattcta tattttcaaa aatggttcca ttaaataacc
     1141 atgtatcttg aagcaccata ccaaacattg aacgcaaatc tttacgtttc atatctctta
     1201 tatctactcc atcaatttta atagcacctt tctttacatc ataaaacctc atcaataagt
     1261 ttattaatgt tgttttacct gctccagtag gtccaacaat agctaccatc tgaccacttt
     1321 ttacttctgc atttaaatct tctattaaag ttgaattttc tccatatcca aaattaacat
     1381 gctcaaatgt aacatttcct tttacatttt ctatcttaac tggattttca acatcaggta
     1441 tttcctcttc ttcgtctaaa atttcaaata cacgttctat agctgcaaaa gaagattgta
     1501 ttactgtaga taactgagtc atttgtgaaa gtggctgatt tatctgccat atataacgca
     1561 caaatgcttg taaatttcct actgttatcc ctccacttac tgcatatata gaaccgacaa
     1621 ctgccacagc tgcaattcca aagtaagtta ctaatgaaac cataggattc atcatacttg
     1681 atataaattg tgccttaaat ccattttcac aaagttcttg atttactttt ttaaattctt
     1741 ttatagaatc ttcttcttta ccatatagtt ttatttcatt aaatccagta tacatttctt
     1801 gtacttttcc atttaatttt ccaagagcat tttgttgcaa tgaaaatcta ctttgagatt
     1861 tttttactac aaatttagat acaaaataac taattggaat tataaaaaga gctataagag
     1921 ccatatatat attaattgta aacatcattg aaagtgccaa aattatgact aagattgagt
     1981 ttacaatttg cataaaactc tgttgcaatg catttgatat tgtatctaca tcatttgata
     2041 tacgacttaa tacatctcca aaactattac tatcaaaata actaataggc aatcttctta
     2101 ttttattttc gacttcattt cttaaatctc tcattgtgtt ttgaatagca ttcgtaagta
     2161 gaaaacttga agtatacgta aaaattgcat tagcaaaata tatacatgct aaaatcacca
     2221 atatttttgt tacataatca aaatttacgg aagcccctgg tactcctttt gcgatactaa
     2281 ttacatcttt agtcaattgt gttattatca atccttcagt tttaggtgca agtgcattca
     2341 tagtagctgc tgaaattata catataatag ctataataaa ggaaaattta tatttcttta
     2401 caaatggaag aagtctacca attgttttct taaaaccttt agactttttc attattctaa
     2461 ttcctccttt tttaactgag atgttgcaat ttcatagtat atttcacagt tttttaataa
     2521 ttctttatga gttcccattc cgacaacttg tccttcattg agtactataa tcttatcagc
     2581 atcaataata gaactaattc tctgggcaac aatcaataca attgcatctt ttgtttcctt
     2641 cttcaattta gcacgaagta ttgcatccgt tttaaagtca agtgcagaaa aactatcatc
     2701 aaaaatgtat atttcaggtc ttcttataat agcccttgca atagaaagtc tttgtttttg
     2761 acctcctgat acatttgctc caccttcact aatcaattca tcaaactttc taggtttttt
     2821 tgatataaac tcatatgctt gtgcaactct tgcggaatat tctaattctg actctcctgc
     2881 cttatgtttc ccaaatctaa tattatttgc tatgctacca gtaaaaagca gtgatttttg
     2941 aggtatgaat cctatctttt ttcttaaagc ttttaaatca tattctctta tatctactcc
     3001 atttatctta atgctaccct ctgttacatc gtaaaatcta ggtatcaagt taattagagt
     3061 acttttccca ctaccagtac ttcctataaa agcgactgtt tcacctgttt ttgcagtaaa
     3121 tgaaatatcc ttaagtacag aagcttctcc atctggatag gtaaaagtta cattatcaaa
     3181 ttctatgata cccttatttt cagtatcctt aattccattt tcaggatttt ttatcaatgg
     3241 ttcttcattt aaaagttctt ttattctatt tgcagatacc tctgctcttg gatacataat
     3301 aaataccatt gaaaaaagca taattgaaaa catggcatgg aaaagatatt ccaaaaatgc
     3361 aaccaactgt ccaacttgaa gctctccaat atttatcttc tcacttgaaa tccaaaatac
     3421 agccaaaaca gctatattta atagtaaaaa gaatgctggt tgagtaattg acataagctt
     3481 aaatagtttc ttagaaacat tagcataata tgtatttgtt ttttcaaatc gctctgtttc
     3541 ataatcatca tttccaaaag ctctaattac tcttattcca gttaaatttt ctctcgatat
     3601 aaggtttaat ttatctagtc ctttttgttg ttttgaagat attgcctgag atacttttgc
     3661 tattattgct actccaataa ttataaaagg tactgatata gccaatacta tagaaagttc
     3721 cacacttgtt ctaattgtca ttattatact tatgatgaac ataactggag ttaataaggc
     3781 agtccttagt agtatattaa caaattgttg aatttgaaat gcatcattcg ttgttcttgt
     3841 aatcattgaa gaaattccaa atttattgta ttcagtatgt gaaaattctt gtgatttctc
     3901 aaatatgtca tttcttatat ctctggttac acttgttgat accttagcag aacaataacc
     3961 caacaatata ctacctacaa cccctataat agaaataata acaatcacta ttcccatttg
     4021 ttttatgtaa gctatattct gatttgcaat accattgtca attattttag ctattatagt
     4081 tggaatcccc aactcgacta atgcaaaact aaatatagcc agtatgttta atactattaa
     4141 taacttataa ttctttaaat aacttaaaat taatttcata tttttctcct ttcttaattt
     4201 ttgagatagt gtaatcatgt aataatacta aatttataaa tgcttaattt tataaaaaaa
     4261 ggagaaaaga gttaacgcac tctcttctcc agcatattct ttttaataca ccctccgaca
     4321 agtcgcttat atgttaacat atttgacaag taatgtcaat tcatatagct aaaagtcttt
     4381 taaatgcttc atctcataac attgtaacaa attggcatgt aatttatttt tgttttttac
     4441 tgtattccaa ttttacaagt tctccaatat tactaacttt cttaaaattc aaattagtct
     4501 tatctatacc agacttaaat agtgaaatcc ctctccccaa tattattggc aatatatata
     4561 tataataatc atcaattaag ttatctttca taaataattt tactatatcg cctccaccaa
     4621 ctatccaaat atctttttta tccttctttt ttccaatttg ctcaattaat tttttaggtt
     4681 ctaaattagt atactttaca ttctccagtt gtcccattaa ttgtatttga gtatatatag
     4741 cattccttac ccttatagta ccattcattt gggcttaatt catttgcaat ctgtacatat
     4801 gtggaatttc ccataatgat agtatcaatt gtatcgtaaa aagtattata atcatcttcc
     4861 tgctcattct taaattctga agatatctcg tctaaaaaac ttacactgcc attctcttca
     4921 gctatataac catctaaact tgtggcaata taaagtacta cttttctcat gtttactacc
     4981 tccaacattt atagtgtgat tattctatgt aaacatcact tttttactca ttcaataata
     5041 ccctattaat aaatcctcaa actaatttaa ataaactttg cataaaacta aaaatatatt
     5101 atataaataa tttaaaatca cactaactta ttgaaaattc taattattac aaataaatat
     5161 tgctctctgt taacgctagt tttataatat aattataagc tattaatatt ataaagccga
     5221 tattactaat ataataatta tagttaattt acttttgttg aaaggtggta aataacatga
     5281 aaatttcaga agtcgctact ccaaattttt tgctagattt agaccagtta gaaaagaata
     5341 tagataaaat tcaacaaatt tgtacaaata ataataaaca actttggcct atgcttaaga
     5401 cacataaaag tacttacata gcgagacttc aaaaaaatgc aggtgctagt ggttttctag
     5461 ttggaacact tgatgaaggt gaagctctga taaaagcagg ttttgaagat attatgttgg
     5521 cttatcctta catgggagaa gtaaatataa atagaattgt gactatggca tcaaaaataa
     5581 atttaatctg tgctacagat aatatagaat gtgctaaggc ttatagtgaa gctttttcta
     5641 aatcaaaaat agagtgcaag ctacttatta ttgtagattg tggcttacac cgttttgggg
     5701 ttgaaccaga aaaagtggtt gaacttgcta ctgaaatttc taagctagaa aacatcaaga
     5761 ttgttggtat aagttcgcat ccaggtcaag tgtacggttg tagttctcca gaaggtgttc
     5821 cagatgtttg tgttcaagaa gattcttcta tggagaaagc atacaaagct ctaactgata
     5881 atggatttaa tatagaaatt gtcgcttctg gtagtactcc aacagttgaa gctgaagctg
     5941 cttctaaaac tataactgct gtaagacctg gaaactatgt atactatgat gctatacaga
     6001 taggtttagg ttgtgctact gaagatatgt gtgctcttac tgttgtgtgt actgtaatct
     6061 caaaaaatag cagtggatat tatttaattg attgtggaag taagtgcctt ggacttgata
     6121 aaggtgcaca tggaaatagt agtattgttg gttatggtag ggtatgtgga catccagaac
     6181 ttactattga ttcactttca gaagaagttg gaaaaattaa agtaaatggt gaaactgata
     6241 taaaaattgg tgatagaata agaattatac ctaatcattc ttgttccact gcaaacttga
     6301 cgagtaattt attaggtttt agaggagata caatagaaaa agtcatagaa gtagatattc
     6361 gtgaaaattc aaaacaaatc atcctttaaa aatatattta ttttgatatt aacatcatga
     6421 aagtatattt ttcttaaaat tattgttaat aactaatgtt aatgttggat aaataaaaaa
     6481 cataaattct agaaatataa atttaaaaaa agaggctgtc tcaaaattgg atatgtccct
     6541 gtcaagtaga cagacttaaa aaaggctatc tttatgcgga atgagttcga tattcaatcg
     6601 gactcattcc
//
LOCUS       DAELXX010000158         6560 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.160, whole genome shotgun sequence.
ACCESSION   DAELXX010000158 DAELXX010000000
VERSION     DAELXX010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6560
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.160"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(214..723)
                     /locus_tag="KPH29_003397"
     CDS             complement(214..723)
                     /locus_tag="KPH29_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBG1468961.1"
                     /translation="MSQQNTGNAIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVVVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(725..1372)
                     /locus_tag="KPH29_003398"
     CDS             complement(725..1372)
                     /locus_tag="KPH29_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468962.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            complement(1386..2558)
                     /locus_tag="KPH29_003399"
     CDS             complement(1386..2558)
                     /locus_tag="KPH29_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG1468963.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNIESYTHCKESVNKLAE
                     RLKLEFEKEGLDCELVDVGENGNTLIGTLGSNIDKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYVIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKGALCAFNMETGLVDNSLCIGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSRENGFGELNAKRLGG
                     SSDASYLTIANVPTICSFGVRGEWNHTSREYAVVDSMFERVKLISTVILNLDKFEV"
     gene            2826..4202
                     /locus_tag="KPH29_003400"
     CDS             2826..4202
                     /locus_tag="KPH29_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468964.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDERDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYDCIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEILNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILADYMNITLRSANRILSILYKAGIATIVNTKLDNQRGRPKKIYKVDFLSFL
                     NKMQKLNS"
     gene            complement(4453..5286)
                     /locus_tag="KPH29_003401"
     CDS             complement(4453..5286)
                     /locus_tag="KPH29_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG1468965.1"
                     /translation="MTIKEVEEKTGLTRSNIRFYENENLIKPLRNERNGYREYSDENV
                     EDIKKIAYLRTLGITIDDIRNIILQKVSLVEVVEKQEKVLETKISDLENAKTMCNKML
                     ESDELSYLNLKVEQYITELPEYWNKNKPVFKLDSVSFLYIWGGKITWGIITIACLLIA
                     ITVFPKLPPQIPIQWNNGVANYVDKKFIYAYPVICVVFRILLRPFIWRWLQIHAFYSD
                     TIADYLTNFMCFVALSIEIFSILFIYNIARHVTIIIFIDILVFIVLLIIGVYKLFKKY
                     N"
     gene            complement(5615..6081)
                     /locus_tag="KPH29_003402"
                     /pseudo
     CDS             complement(5615..6081)
                     /locus_tag="KPH29_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816343.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
     gene            complement(6146..>6560)
                     /locus_tag="KPH29_003403"
     CDS             complement(6146..>6560)
                     /locus_tag="KPH29_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG1468966.1"
                     /translation="KVARLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNMIRN
                     RKLSRLISDVSWSEFIRQLEYKANWYGRQIVKVGKFFASSQICNKCGYKNEEVKDLNI
                     REWICPSCNETHDRDINASINILKEGLRLITIQNK"
BASE COUNT         2257 a         1128 c          704 g         2471 t
ORIGIN      
        1 tttcattgtt gaaattatga aaatatactt gatttaatac caaaagagta tctcatgaac
       61 taaaaagttc attttgagat actctcttct tttttaatta atatacatat taattactat
      121 attagtttat tactatatat caattactat tcattaatta ctattcatca attcttatat
      181 atcatttata attaggttat aattttatgt tatttacatt aatcccataa cagtgagtaa
      241 aactcttact atcaacatcc ccactattgc atcaaatata ggaactgcca acagtaacat
      301 tctatactta gtactggcat ttgctacaag cactattctg gcataatgac caactagagt
      361 acccattaac atacatggca taattaatat ggttgcttgt ggtgcagtga ttagaccttg
      421 tgcaaataag tttgcagctg tagctgctcc tgctgcctta gcaaagaatg ctgctatcaa
      481 aactacaaca gattccccag gtagtccaaa aatacccatt actggaccaa aaatatttcc
      541 taaaatatca actaaaccag ttaattgtaa aaactgcact attacataac ccattatcat
      601 ggctggtaat atctgttcaa cacctatgta aaaacctttt tttgctcccc ccataaatat
      661 ctcaacagca gttttcttag gtttactatt tacaattgca tttccagtat tttgttgact
      721 catactacac agcctcctta gccttattct ttttatttct tacacctatg attaatctaa
      781 ctagattagc accaagtatt ttacagaata tctctataag gattattgga cctaatgcca
      841 ataatgctat tggtaaaagt ggagcttgag tatttattgt gttcagtata acagctgaac
      901 cagcatattg atatgctaca aatatagttc tttcatcatc tgttatttcc ccactttcaa
      961 ataattcttt cgtcattatt gaggctacat ctgaactagt aaatgaagat acaaatgcta
     1021 ttcctgctat tccaggtata ccaagcagag gtctaagtaa tggattaaat atctttgatg
     1081 ctgctttcat tcctcccata gattcaacta catctataag acctacagca aaacagacag
     1141 ttggaatcaa agtcagagca aataagaaac catcttttgc tcccgttcct cctttaccca
     1201 taaagtctac accatctgca atagtaccaa atgaacctgt cagattagca aaatctaatg
     1261 ctttaaggaa gccttgacta tctttaaata ctcctgaaaa taatactatc aatacaaata
     1321 aagataaata ccctttccat gttaccttat tatctccagc tgttacatcc atataaaatc
     1381 cccctttata cctcaaattt atctaagttt aaaataactg tagaaattaa tttcaccctc
     1441 tcaaacatag aatctactac tgcatattct cttgatgtgt ggttccattc tcctcttaca
     1501 ccaaatgaac agattgtagg aacgtttgca atagttaaat aacttgcatc tgaactacct
     1561 ccaagtctct tagcatttaa ttctccaaat ccattttctc ttgacacttc atttacaaat
     1621 gtatgaaatc tcataacatc ttctgtagtt tcaaatggca tcatttcact tataaaatta
     1681 acttctgtag aagttccttc tatgtaagtt tctttacata tctctctaac ttgttcttta
     1741 acattctcca tttcttctac tttttcaaat ctaaggtcta cttttatact acaatcttct
     1801 ggaatagaat tttccactct tccaccctta atcacactta cacttactgt agttccaaca
     1861 tctaaattag tcaatttttg tatgcgtagt atcttattag ccatttcttc tatagcattt
     1921 cttccacctt caaaatcatt acctgcatgt gtttctacac cttttacatg tatattacat
     1981 cctattcttc cttttctacc tatacataaa ctattatcta ctaatccagt ttccatatta
     2041 aaagcacaaa gagcaccttt tgcttccctc aatataacat ctgcaccagt tgaatcttta
     2101 tgacctatct cttcatctcc agaaaatact atttttattg gtctttcttt gtatcctatt
     2161 ttatttaaag ctttaataac gtataaagag attattatac cccccttcat atctaaaaca
     2221 cctgggccat atgcttttcc ttctattatt ttaaatggat tctctccaaa tgtacctgtt
     2281 tcaaagacag tatccatgtg tcctgaaaaa attatcggct ttttatctat attagaacca
     2341 agtgtaccta tcaatgtatt accattttcc ccaacatcta ctagttcaca atccaaacct
     2401 tctttttcaa attcaagctt taatctctca gctaacttgt ttacactttc tttacaatgt
     2461 gtataacttt ctatattaac aatctctttc cataatgaaa ctatttcttc tctattctca
     2521 tcaacaaact tagaaatttc atttaaatat gtattcataa tcttctcctt tattttcaaa
     2581 taaaatgtat ttctttccta aaatctaagt ttattttagg aaatcttaaa agactagtcc
     2641 ttaattacaa aatagcacaa ttattcttgc cagtcaacat atttttcagt cttttttata
     2701 ttttctgatt tttctttcaa ttatcctttt gtaaaataaa ttgacgcgtt ttatctacta
     2761 ttataaaatg aggttataaa ttgattgatt agttacaata ctctcgaaat tttaaaggag
     2821 gttttatgaa atataaaatt ggttttttga caacatctta ttttatgtca gataagttta
     2881 aacaagctct taaagaatta aatgatactt gtgaaattac atttattgag cttaaagggg
     2941 agaatccttt taataattta cctaatatat actatgagca agtagataat tttgatggat
     3001 ttgtatgcag tggtatagtt ccatatagtg aacttataat aaatataaaa gacataaaag
     3061 ttccattaaa ttttttaaag cttgatgaaa gagattttta taaatactta ttcaaattat
     3121 taaatagcca aaaagatatt gactttacaa aatcatttat ggatttttta agagaggaca
     3181 acaattatta tgatatatat tcattaatag actctaataa atgtccatat acaattaaag
     3241 attttaatat ccctcaacct gtatatgact ttaaaaaatt aaatgataaa ttattggaaa
     3301 tacatttaga cttattgcga gataataaga ttaacctttc ttttactaga aattacatca
     3361 ttagttgtga acttaataag cttggatatg attgtattta catggttcca tctacagaat
     3421 caatcctgaa tacatttaaa tctctggtga atgaaataac attacaaaac ttagacaaaa
     3481 ataaatctgc aacttgtata gttacagtaa atagttcaga gtacataaat gaagatttga
     3541 tatttaaaaa tgatgagata caaaatcaaa tatataacac tatattaaat tctctatcaa
     3601 gacatggatt ctatggagtt caggtcaaaa aaaatgatat gaaaatagag atacacacta
     3661 ctaaagaaat attaaacaat atgaccaata actatacaga tttttttatt tcagaagatt
     3721 taaaagaaat taaatatagc ttaaatatag gttggggcat tggaaataca aatattcatg
     3781 cagagcaaaa tgctcgtcag gctaatggta aatctgcttc tttgaatgga aactgtactt
     3841 ttatagtaaa tgatactaat gatacaatag gccctttata ttcaaataaa aattctaata
     3901 atattgaaga tagctcaata gccaataaag ttgcttcttt tatacctcta tctaatacaa
     3961 atgtatctaa aatcatgtgt atgattaatg atagaaattc aaataatgtg tcagcagaaa
     4021 tattagcaga ctatatgaac ataactctta gaagtgctaa tagaatattg agtatcttat
     4081 ataaagcagg aattgcaact attgtaaata caaaactcga taatcaaaga ggaaggccaa
     4141 aaaaaatata taaagttgat ttcttatctt ttctaaataa gatgcagaaa ttaaattctt
     4201 aatatttaat tttttaataa aagcaatgta ttttctctaa ttttaaagaa tcaaaatgtt
     4261 taaattttat tagaatatac aactttttac tttaaaatac ctaaaccttt tatcagtgaa
     4321 ataaaccata atatatcgta gcttattaga aaaacaaaat aatttcatat acaaataata
     4381 aaatagccac tacaatccat aatttgcagt ggctactttt tacactatca cttattactt
     4441 aatttttaaa aattaattgt attttttaaa taatttatac acacctatta tcagtaaaac
     4501 aataaataca agtatatcta taaaaataat tattgtaaca tgtctggcaa tattgtaaat
     4561 aaaaagtata gagaatattt ctatagacaa cgctacaaaa cacataaaat tcgttaaata
     4621 atctgcaata gtatcactat aaaaagcatg tatttgtaac catctccata taaatggacg
     4681 taaaaggatt ctgaatacaa cacaaatgac agggtaggca taaataaatt ttttatctac
     4741 ataattagct acaccattat tccattggat aggaatttga ggtggcaact ttggaaatac
     4801 tgttattgca atcaacaaac aagcaatagt aataattccc caggtaattt tccctcccca
     4861 tatatataag aaacttacag agtcaagttt aaaaacaggt ttgtttttat tccagtattc
     4921 tggcaactca gttatgtatt gctctacttt caaattcaaa taacttagct catcagattc
     4981 taacatttta ttacacatag tttttgcatt ttctaagtct gatattttag tttcgagaac
     5041 tttctcctgt ttctctacaa cttcaactaa agatactttc tgtaatataa tattacggat
     5101 atcatcaata gtaattccta gtgtacgtaa atatgcaatt tttttgatgt cctctacatt
     5161 ttcatctgaa tattctctat agccattacg ctcattacga aggggtttaa ttaaattctc
     5221 gttctcataa aaacgaatat tagagcgtgt caatcctgtt ttttcttcaa cttctttaat
     5281 tgtcatttta aacactacct ctctattcta ttaatttaca actataatat agtcttaaat
     5341 attttcactc tataaaattg tatatttagt atatcttaaa atcaagttaa ttatctttta
     5401 taaattttca tacaataata tctcctttca ataatatctc tttttctata tttaaaacta
     5461 tcttattttc tataaaataa tgtataaaca aggtttacag tcaagtattt ttattatttt
     5521 tttgatttca taaaaaaaag catagcaaat catttgctat acttttttac taaattattt
     5581 attctatatc ttatatgaac ttattgttta actactacac aatactagct ttaatcatca
     5641 tattttttgc aaacatactg gcttcatata tttcttgttc atctggatga ctttgagatt
     5701 cctcatggtt atttaattgt tggcgaatat gttcgttgtc aggagaaagt tttaacatct
     5761 ctttatattt tgctttcaat ccttctgcta tcttaccttg acatatgaat gaacctaata
     5821 tttcattatc ttttgggata attgattcta tcttttctat tatactagct ccataacttc
     5881 cttttttact tgctcccatt gttccaaata gtgctatctt tttattgtga atattttcaa
     5941 gcacttgttt tgaattttta tcacaaattc ccttgtccac ccaatagcct aagaatacta
     6001 tatcataatt ttcatagtca ataccatact gaacctccca tgactaaagt cacagggttc
     6061 ctgcttcata gaattttgca tactaaaata tgtcttacta attctccaca ggctatcccc
     6121 gtagttccta cggttcttac ataagttatt tattttgaat tgttattagt cttaatcctt
     6181 cttttaatat atttatactt gcatttatat ctctatcgtg agtttcatta caactaggac
     6241 aaatccattc tcttatattt aaatccttaa cttcctcgtt tttataccca catttattgc
     6301 atatttgtga acttgcaaag aatttaccta cctttacaat ttgtctacca taccaattag
     6361 ccttgtattc taattgacga ataaattctg accatgatac atctgaaatt aagcgagata
     6421 gtttacgatt tctaatcata ttctttactt gtaaatcttc tatgcaaata atatcgttct
     6481 cttttattaa tttagtagat aatttttgca gaaaatcatt tctttgatta gctatatgtt
     6541 cttgaagtct tgcaactttt
//
LOCUS       DAELXX010000159         6558 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.161, whole genome shotgun sequence.
ACCESSION   DAELXX010000159 DAELXX010000000
VERSION     DAELXX010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6558
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.161"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            121..1023
                     /locus_tag="KPH29_003404"
     CDS             121..1023
                     /locus_tag="KPH29_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBG1468967.1"
                     /translation="MLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIATF
                     GKLLIPFIMAGILGIVMPDVLGSGHNLVEELTSHEYIFGMVVLIFVIRFIFSAVSFGS
                     GAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTG
                     IILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKERGE
                     KILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMT
                     DERDSGAVYDKMENLCKEHTSHIF"
     gene            1632..2645
                     /locus_tag="KPH29_003405"
     CDS             1632..2645
                     /locus_tag="KPH29_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG1468968.1"
                     /translation="MVSSFLNDIFRTILVSCIFIVILLVFRITLFKTFSKKFNYYIWL
                     IVIIKLSLPFMNYTFIFNESKYNGNINKISLGNFNSISIVYSTVLVSVWIAITIFYLV
                     YTLLKYIKLKNMINDLSYDVEDDKIKNLYENLLKELKINKKIRLKYSYEVESPVFFNS
                     CVILPPCDYTLKELNWIFRHELMHFKSKDLYIKYLVIFLKCVYWFNPFVYIMEKFIDL
                     DCEFYCDERVLKNCTIEEKQDYALTIISAMRKGSNSSNKFVAGLHKESDIKKRVFSMF
                     NEKYRNGILIAVILCLLSSITYFEIDLITTKSIFYPLSKQTPIENRTNSKPTYQINVY
                     IDN"
     gene            3001..3570
                     /locus_tag="KPH29_003406"
     CDS             3001..3570
                     /locus_tag="KPH29_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBG1468969.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKSIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVDGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYSNRYN"
     gene            complement(3907..5244)
                     /locus_tag="KPH29_003407"
     CDS             complement(3907..5244)
                     /locus_tag="KPH29_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG1468970.1"
                     /translation="MDTYSLISTSINFILILINIFIAFFLYKLTSYFIDFRNHWIFKI
                     ILILGYSIISQMIIWLSDPVNILFTLFGYFILLIICSKSKLIPIISVVMILYPIIIGL
                     NFLFINNPIYEELIHSKEYILILSIVQIISALIKLLFWFLIHIFLKNRLVNIKQYLSN
                     KIWIFIDMVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAILANI
                     GVILLLQPLIENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIESYLE
                     IEDIAGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNIDIDN
                     HLGIEPIDLCTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKVNPI
                     NRKQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYTENEFSLFLYIRLF"
     gene            complement(5684..6394)
                     /locus_tag="KPH29_003408"
     CDS             complement(5684..6394)
                     /locus_tag="KPH29_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1468971.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELEKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVHIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLEP"
BASE COUNT         2414 a          691 c         1021 g         2432 t
ORIGIN      
        1 taagaaccgt aggaactacg gggatagcct gtggagaatt agtaagacat attttagtat
       61 gcaaaattct atgaagcagg aaccctgtga ctttagtcat gggaggttca gattggaaat
      121 gtgttgccac aagattatta tggattaata gtgattttag gtgttattct tggagttatg
      181 ggggcatttt acaactggtt tactctatat gtgcagtctt tatacaagaa gattcctaaa
      241 attgctacct ttggaaaact gttgattcca tttataatgg ctggtattct tgggattgtt
      301 atgcctgatg ttctggggag tggacacaat ttagttgaag aacttacaag tcatgaatat
      361 atttttggaa tggttgtttt gatttttgtt atacgtttta tattttcggc tgttagtttt
      421 gggtcaggtg caccaggggg aatatttttt ccattgcttg ttttaggagc atttataggt
      481 ggaatatttg gtatggtagg tgttaaagtt tttggaatga atcccgatta tgtaaataac
      541 tttgtgcttt tggctatggc aggatatttt acagctattg tgcgtgcccc tttgactggt
      601 atcatattaa tttttgaaat gacaggttct gttagtcaaa tgctttcact atcagtaatt
      661 tcaattgtgg catatattgt ggctacactt atgaagtcta aacctattta tgaaagtctt
      721 cttgagaggt tacttgaaaa aaatggagaa cctgtacata aagagagggg cgaaaagata
      781 ctagaccagt tctctgttat gtttggctct cctgttgcga acaaggcaat tcaggatgta
      841 cagtggcctg aaaattgtct tttggtagca attcaaagag gtggagaaga aattattcct
      901 agaggtaaga caattcttag accaagtgat gtaattgtga ctatgacaga tgagagagat
      961 agtggtgctg tgtatgataa gatggaaaat ttgtgtaaag aacatacgtc acatattttt
     1021 tagggataat gatgaatgtt taataatgat agaatattta atgaagattt gattaaaaaa
     1081 ttggtaattt gaaagagagt taaggaatta atagaaaatg aaaatgtgat atcttagatg
     1141 taaaagagtt tgaagaaaat aattgtatat atttgttaat gagattatat ggactaatgg
     1201 tatatttata ctgtgctaaa agttgcacta ttgtatgagg aatgggaata aagggtttga
     1261 aaaaataggt agaagatttt tcagatattc ttattgggat attttaatta atatatttta
     1321 attgcgatat tttgggaaga atagaattta tttataaatg aaaataatta aaaggggtta
     1381 aggttgtatt agcccctttt gcttatagag tttggaaatg gtatcttgat agcagttgtt
     1441 ttatgcttat tatattttat tacatatttt aaaattaacc ttatcactcc taaatcatta
     1501 ttctatcaac ttttaaaaca ctttcaagac aatcacctat tgaaaacaaa gttaattaaa
     1561 aacctacata tcagtttagt attcgtgtag atgattatat aaatataaaa gtataagaaa
     1621 ggtgagtatc tgtggttagt tcatttttaa atgatatctt tagaacaatt ttagtaagtt
     1681 gtatttttat agttatttta ttagtcttta gaataacttt atttaagact ttcagtaaaa
     1741 aatttaatta ctatatatgg cttattgtga ttattaagct atcattacca tttatgaatt
     1801 atacttttat atttaatgag tcaaaatata atggtaatat taataagata agtttaggca
     1861 attttaatag tatttctatt gtatatagta ctgttttagt ctctgtttgg attgctatta
     1921 cgatatttta tttagtttat actttattga aatatattaa gcttaaaaat atgataaatg
     1981 atttgtctta tgatgttgag gatgataaga taaaaaatct ttatgaaaat ttattaaaag
     2041 agcttaagat aaataaaaaa atacggctta aatattcata tgaagttgaa agtccagtat
     2101 tttttaattc gtgtgtcatt ttaccaccat gtgattatac attaaaagag ttaaactgga
     2161 tatttaggca tgagttgatg cattttaaga gtaaagacct ttacattaag tatttggtga
     2221 tatttttaaa atgtgtttat tggtttaatc cttttgtgta tataatggag aagtttatag
     2281 atttggattg tgaattttat tgtgatgaaa gagttttgaa gaattgtacg atagaagaga
     2341 agcaggatta tgcattgaca ataattagtg caatgagaaa aggttcaaat agttcaaata
     2401 aatttgtggc tggattacat aaggagtcag atattaaaaa aagagtgttt agtatgttta
     2461 atgaaaagta cagaaatggt attttgatag cagttatttt atgtttatta tcttctatta
     2521 catattttga aattgattta attactacta aatcaatatt ttatccactt tcaaaacaaa
     2581 cacctattga aaatagaact aattcaaaac ctacatatca gattaatgtt tatatagata
     2641 attaaaaaat acaaagttta acaagagatt aatataaata attttaataa gtatctttca
     2701 catatgttaa atttacttta attatattaa agccaatgaa ttattcttta gaagctccaa
     2761 tgccagtaaa tacaggtaaa gaaaaatctt ttactacaat tgtaaattaa aaaatattct
     2821 aatatttaaa gaaatgcaaa agtgtaagaa gggtaaaaat ctgttataat acattaaaaa
     2881 ctaaatttag aaaaatttac ataattagaa aaatgatttg ataaaaaata aagttaaata
     2941 ttaactaagt aaatttgaaa aagaaaaaat ataaagtaca aatttaaaag gaggtaacta
     3001 atgaaaaaga agttggaaga tatgacttta gaggagttat ggaacttgtt tcctatattt
     3061 ttgactactc acaaagaatg ttggaaaaat tggtttaaag aagaacaata tagactaact
     3121 ggaatattgc caatgcaaaa aattgttaga ataagtcata ttggaagtac ttctgtgagt
     3181 acgatttatg ccaagtccat tgttgacata ctgattgaga taattcctga ttataatatg
     3241 aaagatatta aagatttgat tgagtcgaat ggatatattt gtatgtctga gagcagtagt
     3301 aggctttcac ttaataaagg gtatacagta gatgggtttg aagaacgcgt ttttcatttg
     3361 catataaggt atgtaggtga taatgatgag ttgtttttta gagattactt aattgaccac
     3421 ccagatgttg caaaggagta tgaaaaactt aagttggacc tttggaagaa gtatgagcat
     3481 aatcgtgatg cttatacaga tagtaaacta gattttgtta gggagtttac tgaaaaggca
     3541 aaggtgttgt attctaatag atataattag aaataggtac aattagatat ttatcttata
     3601 tatcgtatta tattttttaa gaaagtaatt aatagaaaaa ggggttgttt caaaatggag
     3661 agataaatcc ctttttcatt gttgaaatga taaaaatata tttgatttca tgtaaaaaag
     3721 agtgcctcat caactaaaaa gtttattttg agacacacac atttttatta aggtaaataa
     3781 aaatatcaca tttgattaaa ttttaagtta atgaatatat gtgtaaatat ttaattataa
     3841 gttattataa ttttacatag tataaaacaa tctaatttgt attatataag ataatctaat
     3901 tttagactaa aataatctaa tataaagaaa tagagaaaat tcattctcag tataagagat
     3961 atccaatttt ccattgtact tattaacaat ttctctaata ttctcaatac catatccgtg
     4021 agaattacta tcttttttat ctgaaataaa tagttcttgt tttctattga taggatttac
     4081 cttgttgttt gtcaataaat agctaaaata ctccttctca caacgtgcct ttaataccac
     4141 ttttctattt gacaaatcag gaattttcaa agatgcttct atagcgttat ctaaagaatt
     4201 tgaaaaaata gtacataaat ctataggttc tatacccaag tgattgtcta tatctatatt
     4261 aaagtgcaca tcaacaccat tttcttccaa acttgtatga cgaagattta aaattgagtt
     4321 aagagcttgg tttttgcaaa attgtttgcc actaagagta cctataccta tttttaactg
     4381 attaaaatac tttttagctc cagcaatatc ttcaatttct aaatacgatt caatcatttg
     4441 aaagtgatta ttcatatcat gtcgcatttt gcgtatttga atttgttgat tttctagtaa
     4501 gttatagtat ttttctttta ggtgatttgc ttgtttttct atcttcatct ttacattttc
     4561 aatcaatggc tgtaaaagaa gtatcacacc gatatttgcc agaattgctg acaagatgat
     4621 tagataagtg ccataaggta agttgcctaa gataatattc ttattactac ccatagggat
     4681 taaattagta ggtggcagaa taatagcaat taatattgat aaaaatgaag atatacatac
     4741 catatcaata aaaatccata ttttatttga taagtattgt tttatattga ccaatctatt
     4801 ctttaggaaa atatgtatta aaaaccaaaa taataattta attagagcag aaattatctg
     4861 gactattgaa agtattaaaa tatattcttt tgagtgtata agttcctcat atatagggtt
     4921 gtttataaaa agaaaattaa gcccaattat aataggataa agtatcatga caactgatat
     4981 tataggaatt aatttgcttt tgctacatat tataagtagt ataaaatatc cgaatagtgt
     5041 aaacaaaata tttacggggt cggaaagcca tattatcatt tgactaataa tagagtagcc
     5101 caaaatcaat atgattttga aaatccaatg attcctaaag tctataaaat aggaagtcaa
     5161 cttataaaga aaaaaggcta tgaaaatatt gattaatatt agaataaaat ttatgcttgt
     5221 agagattaaa gaataagtgt ccatttaata tacctttcta ttaattaaga ttctggaaac
     5281 taagttttaa aaaattaaat cttaaaaatt aaaattctgg aaattagatt tcaaagaatt
     5341 gattttgaaa aataaaattc tagaaattaa gcttcaaaaa attgagtttt gaaagattga
     5401 aattttaaaa attaagtttc aaaaaattaa atttgaaaca aaactctaaa aactaagctt
     5461 caaaaaatta aattttgaaa gattgaaatt ctagaaacta agctttaaaa aattaagttt
     5521 caaaaaaact ctaaaaacta agcttcaaaa aattaaattt tgaaagattg aaattctaga
     5581 aattaagctt caaaaaatta aatttgaaaa aacaaaactc taaaaactga gctttaaaaa
     5641 attaagtttc aaaaataaaa ttctagagat tgaacttcaa aaattaaggt tctaacatat
     5701 accttgtaaa ttttcctaca acctcttgat attttttacg gcttatagga attttttcat
     5761 tctgaacttt ggcataagtt tgagttatac tatctacatg gtttagattg acaatatacc
     5821 tttgatgcgt acgtataaaa ttagagggaa gaacagtttc catatcattt aattttccgt
     5881 agtattctaa tgttttatca aaggtaacta cagttaattt tcttagctcg cttttaaagt
     5941 agaggatatc ttttgtatta attttacaaa gattagttcc aatatgaact gtaataaagt
     6001 tttctttcca ttgttctaat ttttttagtg cttcttttaa tactttacat attttttcag
     6061 ttttataagg ttttaagatg tagttcaaag ctcctacatc atacccatga aatacatagt
     6121 cagaaaaacc tgtgacaaag atgattatag tatcttttga atgttttcgt atagtttttg
     6181 ctatgtcaac gccattactt ttttcaagtt caatatctaa aaaaataata tctatgtaat
     6241 aattttcgtg ttctagattg tatactagac tttctccatt tgaatattct tttatttggt
     6301 aggggtagcc ccacaaatcc atttctcttg taatgatttt attcagcatg tttctttgtt
     6361 gtttttcatc atcacaaata caaatcgaag tcaatttttt atctcctttc aagttttata
     6421 tttatatagt taagtatata atatattttg ctacctagat atataaaatg aaataacaat
     6481 tatatccaga tagcaaaata aaatttttta tattagtata cttacactta aaactattct
     6541 attaaactta ttctgaag
//
LOCUS       DAELXX010000160         6556 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.162, whole genome shotgun sequence.
ACCESSION   DAELXX010000160 DAELXX010000000
VERSION     DAELXX010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6556
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.162"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     misc_binding    151..362
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            525..1814
                     /locus_tag="KPH29_003409"
     CDS             525..1814
                     /locus_tag="KPH29_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBG1468972.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     gene            1868..2947
                     /locus_tag="KPH29_003410"
     CDS             1868..2947
                     /locus_tag="KPH29_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HBG1468973.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELCGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            2968..4512
                     /locus_tag="KPH29_003411"
     CDS             2968..4512
                     /locus_tag="KPH29_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBG1468974.1"
                     /translation="MYRVGIDVGGTNTDACILDGELKVIHSVKVATTKDVETGVYNAL
                     KKVIDESKIDHSLIKYAMLGTTHCTNAIVERKRLNNVAMIRIGKPATTAILPFIDWPE
                     DLREKIELDSILVSGGYEFDGRKINELCKDEVVEFCNRIKGKVESVAISGVFAPVNKQ
                     QEEEVAKWVREILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALK
                     DLGIDAQIYFSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGG
                     TTSDLGVLYNGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPES
                     VGYRITEDAIIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLED
                     AVDQMKTSQDDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVE
                     KVYSLDKMSREDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVK
                     VVGDLI"
     gene            complement(4675..5217)
                     /locus_tag="KPH29_003412"
     CDS             complement(4675..5217)
                     /locus_tag="KPH29_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HBG1468975.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDNKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(5365..5649)
                     /locus_tag="KPH29_003413"
     CDS             complement(5365..5649)
                     /locus_tag="KPH29_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG1468976.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(5712..6476)
                     /locus_tag="KPH29_003414"
     CDS             complement(5712..6476)
                     /locus_tag="KPH29_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBG1468977.1"
                     /translation="MNNYLKFATYQLIGFTSSIPSLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAQASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
BASE COUNT         2290 a          807 c         1134 g         2325 t
ORIGIN      
        1 tgaaatttaa aaaaatacat tatataaata tagtaatagt atgaaaattt acatataaaa
       61 ctaaaaaatg taagtaattg actttttaag ttgacaaaat attgcatata tattatgata
      121 taaatcataa attaactaca aatcaatatt atatattcgt agaagaagaa agtagcataa
      181 aggaatttta cagagagtta gatttggctg agaactaata ttggaattta tgtgaaaagg
      241 actttggaga atactattgt aattatgtta gaatagtggt ttaattcgcc ttaagaaaat
      301 gagtgagtaa aattaatttt tactaataag agtggtaaca cggatttata ttcgtctctt
      361 ggatttttcc aagaggcgtt ttttattata aacttatttc agaaaattta aaactatata
      421 ttaaatgaaa attataaaaa tatgatagtt aataaattta taaattagaa aattgtaaaa
      481 tatgctaagg tcagtagttt tacgatttaa atggagggat gaaaatggaa gaagaaaaaa
      541 ttaactatgc tctggaacag attccagaga atcaaaaacg tggttgggta gctatgtttt
      601 cagttttagt agcaataggt gtggatttat catctgttat tttaggcgct gagttggcac
      661 agagtatgcc aatgaaacaa gcaatattgt cagtaattgt tggttcattt ttttctgcaa
      721 ttttgtatac tacatgctct ttggttggtt catctacaag tttatctaca tctatgataa
      781 caaagtatgt atttggagaa gctggagcaa aaatattttc tctagttata ggggtttctc
      841 tattaggttg gtttggagtt caagtaggtt tctttgcaca aaatgcacag ataataataa
      901 aagatatatt taatttagat gtcagtatgc agattttaag cctaataggt ggtctcttga
      961 tgatgagtac tgcaatttat ggatataagg ccatggagaa gttgagtgtg tattcagtac
     1021 catttttatt agttcttatg atgctaacta tattcttagc ttttagagca aatggaattt
     1081 cagttgatga taatatgaaa tctactatga catttgcagg tggagtttct ctgtcaatga
     1141 gtataataat agttggtgca attgtatctc ctgatataag tcgttgggct aaaagtagaa
     1201 gagactgtgc tctttcaagc tttttgggaa tacaatttgg aaatgctttt atgataatag
     1261 tttcgatagt attagtaaaa tgtatgggaa cttcagatat tatgagaatt tttattacat
     1321 taggaattgc tataccaggt ataatagtac ttacattagc acaatggact acaaatacaa
     1381 gtaatgtata ttcagcatca ttaagtatag ctcttgtact taagaaggct cctgagaaag
     1441 tattaactat agtccttggt attatagcaa cattattggc tgtatttgga atatatgaag
     1501 gttttatagg atttttaaat ttacttggaa ttgtaattgc acctgttggt ggtgtatata
     1561 cagcagaata ttacatagta aagcaagaac ttaaaggctt tgataaagga gtattatata
     1621 aaccaatagt taaacgttca atagtgtctt ggataattgg tatattaata acttaccttt
     1681 caacatatgg atttataact cttactacta tagcaccttt agatggtttt atagcagggt
     1741 ttgtagtaca atctattatt ggaaaagtgc tttgttcaac aaaaaataag caagaaatag
     1801 acaaagcagt ataaagaaat ttgtttataa agtataaaat aaattagaaa tatgaggaga
     1861 aagtaacatg agatttttaa cagaagaaag tgtagataaa atagcagttg gtgcagcagt
     1921 gcttggaact ggaggtggag gtgacccata tgtaggtaaa ttggttgcaa aacaagccat
     1981 taaaaagtat ggtcctgtaa aagtaataag tttagatgaa cttgatgatg atgctttggt
     2041 agttccagta tctggtatgg gttctcctgt aattacaata gaaaaattac tttcagaagt
     2101 agagttaact acccctttag aaataatgga gaaattatta aatagaaaag tggatgttat
     2161 aattcctata gaaataggag ggataaactc acttatgcct atagcagttg ctgctaaaaa
     2221 aggacttcct atcttagatg cagattctat gggtagagct ttccctgaag ctcaaatggt
     2281 aactttttat cttgaaggat atgaggcatc tcctgccgta atgtctgatg aaaaaggtaa
     2341 ttctgccatc ctttatcctg tggatggaat ttggtcagaa agattagcta gaactttaac
     2401 tgtagaaatg ggaggaagtt cttctattag tgactataat ttaagtggag ctcaagttaa
     2461 aaaagctgct ataggtaata ctcttacaat agctgaaact ataggagggt ttttacttga
     2521 aaataaatct gattcaaaag gtgctgttga gaatatacta aaagaattgt gtggatacaa
     2581 gttatttgaa ggaaaagtaa ttgatattaa gagagagttg aaaggtggat ttactagagg
     2641 acatgccttt tttgctggga ttaatgagta tgatggagag tattctatat tattccaaaa
     2701 tgagaattta attgccaaaa aaggtgatac accactttgt attacaccag atttaatagc
     2761 agtattagat ttagaaactg gatttccaat aactacagag agaataaaat atggctctcg
     2821 tgttatggta gtagcttttc cttgtaatga aaagtggaga acagaaaaag gaattgagac
     2881 agttggacct ggatactttg gatatgatgt ggaatataaa acagtagaag aattacaagg
     2941 aaaataaaag tttaggagga aattttgatg tatcgtgtag gaattgacgt tggaggaaca
     3001 aatacagatg cttgtatact ggatggggaa ttaaaagtaa ttcattcagt taaggtggca
     3061 actacaaaag atgttgaaac tggtgtttac aatgccttaa aaaaagttat agatgaaagt
     3121 aagattgacc attcattaat aaaatatgct atgcttggta caactcattg tacaaatgct
     3181 attgtagaaa gaaaaagact taataatgta gctatgataa gaataggaaa gccggccact
     3241 actgccattc ttccatttat agattggcca gaagacttaa gagaaaaaat agaattagat
     3301 tcaatattgg tatctggtgg atatgagttt gatggtagaa agataaatga actttgtaaa
     3361 gatgaagttg tagagttttg caatagaata aaaggcaaag tggaaagtgt tgcaataagt
     3421 ggagtatttg ctccagtaaa taaacaacaa gaagaagaag ttgcaaaatg ggttagagaa
     3481 attttagggg atattcctat atctttatca aatgaaatag gtaatatagg tatattagaa
     3541 agagaaaatg gagctatttt aaattctgca ttgtctcaag taggtaaagc tgtatcttta
     3601 ggatttgaaa aagcacttaa agacttaggt atagatgctc aaatatattt cagtcaaaat
     3661 gatggaacat taatgaattt agagtataca atgaaatacc caatttttac aattggatgt
     3721 ggaataacaa attctataag aggagcttcc ttcttatcaa aagtaaaaaa tgcaatagta
     3781 ttagatgttg gtggaactac aagcgacttg ggtgtactat ataatggatt ccctagagaa
     3841 tcatctattc cagttacaat tggaggagta catacaaact ttagaatgcc agatgtttta
     3901 actattggtc ttgctggtgg tacatgtgta aaaggcgaaa aagataatat aaagataggt
     3961 cctgaaagtg tgggatatag aataactgaa gatgcaataa tatttggtgg agacactcta
     4021 actttatcag atgttgttac aaaattagat atggcgtttg aagatagaaa aagcaatgtt
     4081 gaaaacttag ataaagagta ttgtaatgag gtatataaga aggttatttt aaaattagaa
     4141 gatgctgtag accaaatgaa gactagtcaa gatgatgttg aattggtttt aactggtgga
     4201 ggaagtataa ttgttcctga aaaactaaaa ggcataagca atgttataag acctcctcat
     4261 tatactgctg caaatgctat tggtgcagct ttaggacaaa taagtggaga tgtagaaaaa
     4321 gtatacagtt tagataaaat gtcgagagaa gatgccatag aagatgcaaa aaaagaggct
     4381 gtaaataaag ctctaaaagc tggtgctaaa gaagatacta tagaagtatt aaatatagaa
     4441 gatgttccac ttgcatacct tcctggtaat gcacttctta taaaagtaaa agttgttggt
     4501 gatttaattt aaagaataaa tataagtatt tccagaatta tataaatatt tctggactca
     4561 tttttaaact tattttatca ataaaaacag cataactttt gactatattc ttataagttt
     4621 ccgaacagcc gatatatacc tattttttga ggtttatcgg ctgtttgtta tcttttatcg
     4681 cattccaact atttttctta ccgtatttgc tgtacgaatt gtaatcttat cacttacatt
     4741 tgaactggca gtctttgacc accaatttgt ctttttataa tctttacgac taaaagacca
     4801 aaagatagca tttttataat gatatacttt ttcatactct tctttaggat ttccaacctc
     4861 atcataaaac tcgtcataag ataagtcagg aaacataaaa ataagattat catatatttc
     4921 tttgttgtta tctccccacc aattaggagc attatttagt atatctttta actcctcttg
     4981 tgaaataata aaaactggta tttccaattc aaactgtttt tttatcatta atcttattgt
     5041 atttgagata accttgttgt catctacatc acttaaaaag acaacattac cactattaag
     5101 gtatgtagaa acttctgaat aaccaagttc tacaaagctt gcttttaatt cagacataat
     5161 tatcttgttc ttaccactta tattgatacc tcttaataat gcgatataac ttttcatata
     5221 ataagttcac ctctaaacat tcacattttt ctatgtagta caaactggaa tttgcatctt
     5281 ttataattct ttcaaaactt cttcatatgt aattttttta atataaaaaa atcggtgctt
     5341 aatttgtacc gatttttatt tttcttagta aagaactcgt ttttttgctt ctgctatagc
     5401 tctagcagca ctttttgctt gcattctttt ttcttcttct tttttaaatt catttattgt
     5461 ttcatttaga ttcttatcat gttcattttt ttctttatct tcgttttctc tgtatactaa
     5521 tatataatct attattgatt cgtttattat ctgagatata cttttatttt cattaattcc
     5581 tattctctta acttccttaa gtagagtctc atcaatatga accgttgttt gctttgtatt
     5641 ttttgccacc ttaatcacct cttaaaaaaa attctaacat taaaattttt tttagtcaat
     5701 aaattttaat attaaaattc tttaaatgtt ttatttgatt ttctaaaatt ttctattgta
     5761 tttatcatct tattttttat attttcacct agtacagaag actttagtct aggattttct
     5821 atatggtcat aaattggttc taaaaattcg acttcgacag gaacagagtt aattttacca
     5881 ataggttggt atccttcata agtatctttt gaatttttta ttacgagtgg aacaataggg
     5941 cattttgctt tatatgctat ctttaaagct ccacttctaa actccgaaac caatgaattt
     6001 ggttctttaa tccatgttaa atctccttct ggaaacactg ccatactttg acctgtaagt
     6061 atattttgag aggcttgggc tatactcttt ataccctcac gattgttttt tctgtttaca
     6121 tatacacatc ttagtaattt tgcccattct ttaaatatag gtatatttct ccaaactggt
     6181 tcatctgcaa ttacacatcc aattggtctt tctacacttg ctgttagtat gaagctgtca
     6241 agcatactag aatgatttac aacaaataga agtggttctt ttggcagtgt ttccttacca
     6301 attatattaa gctctatgtt cactatatct aaagattttt tagcttgttt ttgcataaat
     6361 tcaaattttt cctttaagct aaatttatca ggattctttt taattttaat aaggctaggt
     6421 atagaagatg taaaacctat aagttgatat gtagcaaatt ttaagtaatt attcataaaa
     6481 ttatctcctt aaaataaaat tatttattac tgtaaagatt ttaacataaa ataaaattta
     6541 agtctatttt aattca
//
LOCUS       DAELXX010000161         6350 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.163, whole genome shotgun sequence.
ACCESSION   DAELXX010000161 DAELXX010000000
VERSION     DAELXX010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6350
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.163"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(52..1149)
                     /gene="ychF"
                     /locus_tag="KPH29_003415"
     CDS             complement(52..1149)
                     /gene="ychF"
                     /locus_tag="KPH29_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBG1468978.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
     gene            1465..2730
                     /locus_tag="KPH29_003416"
     CDS             1465..2730
                     /locus_tag="KPH29_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBG1468979.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSAKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
     gene            2745..4031
                     /locus_tag="KPH29_003417"
     CDS             2745..4031
                     /locus_tag="KPH29_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBG1468980.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDKLSKSIERFYPEEPESVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LDYLDVIKDITFEEVEERLKEHFKEEYCVISIVEPK"
     gene            4428..5174
                     /locus_tag="KPH29_003418"
     CDS             4428..5174
                     /locus_tag="KPH29_003418"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBG1468981.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKDDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNKHNISEE"
     gene            5204..6139
                     /gene="rsmH"
                     /locus_tag="KPH29_003419"
     CDS             5204..6139
                     /gene="rsmH"
                     /locus_tag="KPH29_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBG1468982.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGKLVDIIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNKGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
BASE COUNT         2474 a          664 c         1076 g         2136 t
ORIGIN      
        1 tagtttttta gttattatat ataatagtca aagtgtatat gattaatcat tttaaacatt
       61 aaatctaaat aatattacat ctccatcttt aactatgtat tctttacctt ctaatttaac
      121 caaccctttt tctttagcag cagccatagt accatgctca accaaatcat taaaggctat
      181 tgtttctgct ctgataaatc ctctttctat atcagaatgt atttttcctc ctgcctgagg
      241 agctttactt ccaactttta tagtccatgc tctaacttct tttggaccag cagttaagaa
      301 tgaaattaaa ccaagtaaag cataagaaga ttttataagc ttatcaagcc ctgattgttc
      361 aagacctaat gtttctaaaa actctttctt ttcttcagca gaatcaagtt cagaaatttc
      421 tgcttctatt tgtgcacata ctacaacaac ttcggcatct tctgtttctg caaatgcttt
      481 aacttgttga acatatttat tattttctcc attatcagct aaatcatctt ctgatacatt
      541 tgaagcatat attacaggct tagatgtaag taaatccaat gaatttacga atgtttgttc
      601 atcttctgta aattccatag ttcttacaca cttactttct tctaaagtag acattattga
      661 ttttaataaa tcaagttcag aacctaaagt tttatcagcc ttagcagctt tttgagtttt
      721 attaattctt ctttcaagaa tctctatatc tgagaaaatt aattctaagt ttatagtttc
      781 tatatcgcga agtgggtcta cagagccatc tacatgtaca acattttcat cctcaaaaca
      841 tctaacaaca tgaactattg catcaacttc tcttatatgt gataagaatt tatttcctaa
      901 accttctcct ttagaagcac ctctaactaa acctgctata tcacaaaatt ctatagcagt
      961 aggaactatt ttttcagaat tatataattc ttgtagtttg tttaatcttt catctggaac
     1021 agatacaact cccacatttg ggtctattgt acaaaaaggg taatttgcag attctgcacc
     1081 tgcttgagtt attgcgttaa atagtgtgct tttacctaca tttggcaaac caactatacc
     1141 taatttcata ttaatcgtcc tctctcttat tcttttttat attctttata taactttaaa
     1201 caatcaatct ataggattgc aaaattgttt taattataga catacatatt gattatacat
     1261 catatatatc aagcttgtaa actagacttt aaagcatatt gatttaaata atatttgcat
     1321 ttaatttaaa taaaaatatt gcttttagaa aataataaaa aggatataat taaatagaag
     1381 gtttttatat gtgagtatat ataattaaaa aataaataaa tttcaatttt aaaattgatg
     1441 tttaaataaa aataggaggt acatatggtt aatactaaaa aaataataaa tttaggtaat
     1501 aatattaatc ttactttgat aaaaacagaa aagtttaaat cgaatcttat aagtttatat
     1561 gttcagagac tattggatga aaaagaaact acaaaaaatg ctctaatacc atcaataata
     1621 gcaagtggaa gcgctaaata tcctagtgct agagcaatat ccaataaatt agatgattta
     1681 tatggttcat ctatgggagc tgatgctgta aaacgtggag aacgtcaagt attgagtttt
     1741 aaagtaatta acataagtga aaagtattta gatgagagta tatttgaaga agtagtagaa
     1801 ttttttaatg aagtaataaa taatacgctt gttgtagatg gtgggtttaa agaagaatat
     1861 ttaaatattg aaaaagagaa tttaagagaa aagatacaat caataataaa tgataaaaag
     1921 gaatatgcgc aagataaatg tattgaggct atgtgtaaag gagagagata tagtgtaagt
     1981 gaatttggtt atgaagatga aatagatagt ataacttcaa gagagttata tgaacattat
     2041 aaaaacatac ttaaaacatc acctatagat atagttgtag aaggtaattt tgatgaagat
     2101 aaagttgtag atataataag taaaaatcta aaatttgaaa gagaagaaat tataaatata
     2161 ccaagagcag attttataaa aaatgtggat gaggtaaaag ttatagatga acaaatggaa
     2221 ataactcaag gtaagttagt tatgggttat agagcaaatg tggattatgc agacatagat
     2281 aaatattatg ctttagtagt tgggagcaat gtcttaggtg gtggtccaca ctctaaatta
     2341 tttgttaatg taagagaaaa agaaagttta tgttactata tattctcttc aatagagaag
     2401 tataaaagta tcatgtttat atcatctggt atagaaacta aagattatga taaagctgta
     2461 gaattgataa aaaaacaagt agaaagtgta aaagcaggtg atatatctga tgaagaatta
     2521 gaaaacagta aacttgcttt agttaattct atgaaatcta taactgataa tataggtggt
     2581 atgtctgatt ttgttttctc tcagtctatg gcaaagacaa attctgaagt acaagacata
     2641 atatctagta tagaaaaggt tacaaaaaaa gatatagttg aagctataaa aaatatagag
     2701 cttgatactg tatacttctt gagaaattaa tggaggagat gtctatggaa aaaatagtta
     2761 atgatatatt gaaagaagaa gtttattatg agaaattgca aaatggatta gatgtatatt
     2821 ttatgcctaa aaggggtttt atgaaaaagt atgctatttt ggctactaat tatggttcaa
     2881 atgacttaga atttgtacca attggagaag ataagaaaat tagagtgaat gaaggtattg
     2941 cacattttct agagcataag atgtttgagc aacctgatgg aggagatgca tttgataaat
     3001 tttcaaaatt aggagtaaat gcaaatgcat ttactaattt tacaatgaca gcctacttat
     3061 tttctgctac tgaaaatttt tatgagagtt tagaacatct aattgactat gttcaaacac
     3121 cttattttac agatgaaaat gttgaaaagg aaaaaggtat aatagcacaa gagataaaaa
     3181 tgtacaatga tgaccctgat tggaatgtat actttaattg cttgaaggca atgtatgtaa
     3241 attatcctgc tagaattgat atagcaggta ctgttgatag tatatataag ataactaagg
     3301 aagagctata taaatgttat aatactttct ataatccagg aaatatggct ttatttgttg
     3361 ttggtgactt agatgtagaa aaagttatag atgtaactaa aaagtcaaac aattacaaag
     3421 tagacaaact ctctaagagt atagaaagat tttatccaga agaaccagaa agtgtaaaag
     3481 aaaaagaggt tatagaaaaa ttccctatat ctatgcctat gttcaacata ggttttaaag
     3541 atagtaatgt tggtttaaaa gggaaagaac ttttacgtaa agagattgta actgatatac
     3601 ttgtagggat gttatttaaa aaaggaagta agctctatga agatttgtat atgcaaggtt
     3661 taataaatga aaattttgga gctggatttt cgtctcaagt ggattatgct tttagcataa
     3721 tagctggaga ttctaaagag cctaaaaaag taaaagaaat aattcttgat tatatagaaa
     3781 aatcaaaaaa agaaggatta tcaaaagaag agtttgagag aactaaaaag aaaaaaatag
     3841 ggtcatttat taagtgcttt gattctataa actttatagg taatagtttt atatcttatg
     3901 tttttaagga tataaatcta ttggattatt tagatgttat taaagatatt acttttgagg
     3961 aagttgaaga aagattaaaa gagcatttta aagaagagta ttgtgttatt tctattgtag
     4021 aacctaaata gaaacttaat cataataaga ataactaatt aagtattatt tatgttttta
     4081 aatgtttatt ttggtaaaac aaatttataa aatatattta tataaattga ggtaaaaatt
     4141 taagagctat agataaggaa tagaagaggt tgatgtatat atgcatcaat ctttttgatt
     4201 aaaaaatatc tatgtacact tgtaacttta gtcatgagag attcagttta aaaaatttta
     4261 tagtaattat attagtataa tatagtgtag ggatttgtag aataaatttt aatatttttt
     4321 gtagaattaa cattaatgtg ttatcataat ttagtataat tacaattaat tgtaattata
     4381 gcatattgct ttggaaatat tttcaattaa tacaaagagg tgacgttatg gatagagtag
     4441 catttacact gtttggcata gacatcatgt ggtatggaat acttatggca tgtggaatga
     4501 ttttaggaac tttaatagca ataaaagaag caaaaagagt gggaattaaa gatgatgatg
     4561 tattaaatat agctataata gcaattccag ttggactaat ctgtgcaaga atctattatg
     4621 ttgtatttaa ttggagttac tatgctcaaa atatgtctca aatttttaat tttagaggtg
     4681 gtggacttgc tatacatgga gggttaattg gtggaatatt agctggttat atatacacta
     4741 agattaaaaa tattaacttc ttaaaaatgg ctgatacagt tatacttgga atgcctttag
     4801 cacaagctat tggaagatgg ggaaatttta ttaatggaga agcacatgga ggtgctacca
     4861 atttaccttg gggaataatg gttgatggag taaaggtaca tccaactttt ttatatgagt
     4921 ccatatggga ttttggaata tttatagttt tattactatt tagaaaaaat aaaaaatatg
     4981 aaggtcaagt tatagttact tatataactt tatattcaat aggaagattt tttatagagg
     5041 gattaagaac agatagttta atgttaggac cacttagaat ggcacaagtt ataagtctaa
     5101 ttggagttat tggtggcata attgcacatg tttatttatc aaagaaaaat aaacataata
     5161 ttagtgaaga atagtttaaa aagtgaattt aagggaggta actatggaat ttcatcatgt
     5221 atcagttctt ttaaatgagt gtatagagaa tttaaatata aagccagatg gcgtgtatgt
     5281 tgactgtact atgggaggag caggacattc taaagaaata gtaaaaaaac tgtcagataa
     5341 gggattattt atagggtttg accaagataa aaatgctata agcacagcaa aagagagatt
     5401 gagtgaatat gaaagtagag tgaagtttgt tcacagcaat tttgaaaata taaaagagga
     5461 attagaaaaa ataggagttt acaaaattga tggtgtatta gctgatttag gagtttcatc
     5521 tcatcaactt gatgaagctg atagaggatt ttcttatatg caagatgcac cacttgatat
     5581 gagaatggat gttaggtgtg agttttctgc atatgatgtt gtaaatactt atacagaaga
     5641 tgaattaact aagataataa aagattatgg agaagataac tgggctaagc gtatagctaa
     5701 atttatagtt gaagaaagag caaacaaacc aatagaaact acaggaaaat tagtagatat
     5761 aattaaaaaa gctatcccta agaaagctag aatagatgga cctcacccag ctaaaagaac
     5821 ttttcaggca atacgaatag aagtaaataa tgaacttgga gtaattacaa agatgataaa
     5881 tgatgcttct tctataatga ataaaggtgg aagaatatgt ataataacat tccattcatt
     5941 agaagataga atagtgaaaa atgcatttaa gcatttagca tcagattgta tatgtccaca
     6001 acatttacct atatgccaat gtgataaaga atctgaggtg aaaataataa ctagaaaacc
     6061 tatattacct agtgaggaag aaatagaagt aaatccacgt tcaagaagtg ctaagctaag
     6121 agtagcagaa aaaatataag tagttataag gctaaagaat aatcttatat ataaaataag
     6181 attactataa ttaattaaaa agtattatat attttgttga actggtaaag caattaaaaa
     6241 acaattaaca ctataagaaa tgttctttgt tattgagata tataatgaaa atttagatat
     6301 aaaaatatag atatagatgg tattaagtaa tataatggag aacttaggaa
//
LOCUS       DAELXX010000162         6292 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.164, whole genome shotgun sequence.
ACCESSION   DAELXX010000162 DAELXX010000000
VERSION     DAELXX010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6292
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.164"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            395..1762
                     /locus_tag="KPH29_003420"
     CDS             395..1762
                     /locus_tag="KPH29_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBG1468983.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILNYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            1787..2395
                     /locus_tag="KPH29_003421"
     CDS             1787..2395
                     /locus_tag="KPH29_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HBG1468984.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGKNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMNDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYEKLTKV"
     gene            2443..2814
                     /locus_tag="KPH29_003422"
     CDS             2443..2814
                     /locus_tag="KPH29_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBG1468985.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            2993..3331
                     /locus_tag="KPH29_003423"
     CDS             2993..3331
                     /locus_tag="KPH29_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBG1468986.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
     gene            3798..4301
                     /locus_tag="KPH29_003424"
     CDS             3798..4301
                     /locus_tag="KPH29_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HBG1468987.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGAYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            4348..5307
                     /gene="moaA"
                     /locus_tag="KPH29_003425"
     CDS             4348..5307
                     /gene="moaA"
                     /locus_tag="KPH29_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBG1468988.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSVTRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            5311..5781
                     /gene="moaC"
                     /locus_tag="KPH29_003426"
     CDS             5311..5781
                     /gene="moaC"
                     /locus_tag="KPH29_003426"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBG1468989.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDYMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            5817..6248
                     /locus_tag="KPH29_003427"
     CDS             5817..6248
                     /locus_tag="KPH29_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HBG1468990.1"
                     /translation="MAKVVSINISEKKGVIKVPVPCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
BASE COUNT         2647 a          511 c         1134 g         2000 t
ORIGIN      
        1 ttgtattttg aattaatatc tacttagaca ttgttttaat tgttatattt tcatttaatt
       61 aagcattgat ttatcgtttt ttatatttca tataacaatg caaaaaataa tttttatata
      121 gtaaatgaca aaagtttaca ataaatgtat attttcttac tcattcctta aaattactca
      181 tttagtttaa taactctcat ttcatatgta aagttggtaa aaaaattaaa attaaaagta
      241 aaaaactaaa taaattaatg ttaatttgaa atatatatga aggtttttgg agaatactta
      301 cttatgatag gaaaaaatgt taaaataata gtgtaatcaa atataaagga ctgaaataat
      361 taacagttat taaatattag gagtgtaaaa atttatggga ttaaaagaat ttgattttat
      421 tgaagagtct attgatatgt tgaggacaat gtcaccaaca ttagaaacta tatcagatga
      481 aatagaagag tattttgaaa atatcttgga tgaaaagaat caagaatata taaatgtaac
      541 ctctagaata aaatcagaat caagtttaag agaaaagata attagaaata gatatttaaa
      601 aaaatatgga gaagcaagta atcttattca taatgtttct gacttaatag gcctaagaat
      661 tgagtgtaga tttattgagg atgaaaacaa aatatataga cttttgagaa gatattttaa
      721 taaaacagat gacaaaataa actattataa taaagagaat aagaatatta aattaaaact
      781 atctgagagg caacccaata agcaaaaaaa tggatttgaa atatataaaa tagatggagt
      841 ttttgcgtat ttagatagag aagtaaaatt tgaacttcaa attaaatctc ttgtaaatgt
      901 gttttggagt gaaatagaac ataaaataat ttataaaaat aatacatatt tacttgctga
      961 taaatttata aaggatatga tggactctat aaagaataat cttactatga tagataatca
     1021 gcttttaagt atttataaaa actttcattc agggaagtct tttaatatga aagtaagtaa
     1081 aaaagaaatt gaaaaattat ttgcgaaact agtctatgac gctttttctg aaaagatgaa
     1141 taaaagtata ggttttgttg tagattttaa aaaaccatgt gaaactatat tgaactattc
     1201 ttttaataaa caagatataa gtgatgaagt tttaggtaat tttatgcttg atgagtttgc
     1261 aagattaaat gaaattgtaa acaaggatat agattttaat gagcaaatag aatttgaaag
     1321 agaaccaaca tttgatgata agttttgtaa agacctagga aatcatttta gaagtagatt
     1381 aaacacagaa tttccatgga acctattttt tagaatatta tttgaaatag agccatataa
     1441 caataccaaa gactttgaaa actttgtaga gtttataaaa gaaaatgtgc tactagaaga
     1501 aaatagggag caattattat gccaatttga agaagatagc aagcttataa ttgaagatat
     1561 atatgattgt atatttaata gtataagtga gattgatagt gttgaaattt tatataacta
     1621 taatcttgaa aaaataaact ttacatcaag tgaaatcata aattgtatat gtagagaata
     1681 tgagtgttat ggtgaatact tagaagaaaa agaaaagctt atgaatacat ttaaagaaaa
     1741 gattattcaa atctttgagt aataggtcaa tgctgaagtg gcgctaatgc gccatttttc
     1801 aaatatatat gaattaagta aaattatttt ggaggaggcg atttgtatag agaaaacagg
     1861 tgtaattttg gcagggggaa aaaattctag aatgggtaga gataaagcct ttttagagtt
     1921 acatgataag ctttttattg aaattgcaat agaagcattt aaaaactttg atgagttgat
     1981 aataatatct aataatgaag aattatattc taaatacgat attaaagttt ataatgatat
     2041 agtaaaagat gttggtccaa ttggaggaat atatactgct cttgaatatg caaagtatga
     2101 tatagttaca attgcatgtg atatgccata tctaaataat caaatagtcg aaagaatagc
     2161 taataaaatg aatgataaaa gtgtgatttc agtgacaaat ggaaagttac aaccattgtg
     2221 ttctggatat aaaaagagta taataaataa agtatcttta tgtataaggg aaaatgattt
     2281 aaagctcaga agctttatag ataaaataga taaaagttat atatactttg atgaagaaga
     2341 tttattttta aatgtaaaca ctgttgatga gtatgaaaaa ttaactaaag tttaattagc
     2401 tatggttgta aaatagagta ggatttatat ggaggagagt ttatgaattt acttaaatgc
     2461 gattcttggg cagtaatatt aaataatagc gataaagaaa gtaaagcata taaaatatta
     2521 gatgaactta aaaggaatat gtacaaagtt gttgctatag atgaagagaa aaaacctatt
     2581 gaaggaatag atgtgtatga atgtttaaaa gatgttccac acaatattga tgttgtagct
     2641 attattgata aacaatctaa gatggatgtg attttagaag aagtagagct tttggatata
     2701 caaaatatgt ggtttgaaaa aggaagcttt agtgaaatga tgattaaaaa gactaaagat
     2761 ttaaaattaa atatagaata taatttaagt ttatatgatg agttgactag ataaaattat
     2821 atttcttaat aataaaaata taattagtta ataaaacata ttattttata gtatagattg
     2881 gttaataaaa gtacagattg gttaatgaaa cattaaatta caatatttgt taaaagaatt
     2941 gtgagttaca tgatataatg tattaaatag gatactaagg agttgatata ttatgttaat
     3001 agtaactaca gaaaaagtag aaggtaaaaa gatatcaaag gttttaggat tagtgagagg
     3061 aagtacaata agagcaaaac atgttggaaa agatatagga gcaagtttta agaatcttgt
     3121 tggaggagaa cttactggat ataatgaaat gctcactgaa gcaagacaaa ttgccatagg
     3181 tagaatggtt gaagatgcag aagctaaagg tgcaaacgca gtaatagcat ttagactgtc
     3241 ttcagcttca gttatgcaag gggcagcaga aatgcttgct tatggaacag cagttgtttt
     3301 agaagatgat aatagtattc ttgaaaaata aaatataaat agttaaaaag aggcatcttt
     3361 taaaaatttt atggatgcct ttttttattt gaaaaaacta atttgagaag aaattttaac
     3421 tattatgaca ataaaattag ggtgatatta tataaataag taaaattata ttatataatg
     3481 tatggtatag actttaaaaa ttttatttgt agagaaaaat atggacaatg ttataataat
     3541 tatagaaatt aaaagtactt acattcatta tgtatgtgac atgtataatt agttttatat
     3601 ttaatacagt tttgaagtaa tatcttcatt ttgagaacgt tggaataata aaaaatgtat
     3661 gtattatagg tgatataaat aaaaaataaa tataatatgt tatattgctg atagaaaaga
     3721 gaataaaaga aaataggttt tattaaaaga gatttttatt tataggactt aatcaatgta
     3781 tagaggggtg agaacatatg tttaatgttg caattataac acttagtgat aaaggatatg
     3841 aaggaaaaag agaagatata acaggaaaaa agttaactga atttgttgaa aatacaggag
     3901 cttataaagt tactgagtat gtacttataa aagatgataa ggaaatgcta aaggaaaata
     3961 ttataagact ttgtaatagt aataaaatag atttaattct tacaaatgga ggtactggtt
     4021 tttcaaaaag agatataacc ccagaagcaa ctaaggaagt gttggaaaaa gaaataccag
     4081 gtctgagcga atatatgaga atgaaatcca cagaaattac taaaaaggcc atactaagta
     4141 ggggtgtgag tggaataaga aataattcta taataataaa tttacctgga agtcctaaag
     4201 gtgcagttga aaatctaagt tttataattg atgttttaga ccatggcata gaagttttaa
     4261 gaggagaagc tacagaatgt gccactaaaa aaagtgaata gtatataatc tagatagata
     4321 aaaaatagac aaagaggtag ataaagtatg attgataagt atggaagaaa aatagactac
     4381 cttcgtattt cacttacaga taagtgtaat ttaaggtgtg tctattgtat gccaccagat
     4441 gtaaaatttg ataaaaatta tataaatgaa aatctaagct ttgaagacta caagtttatt
     4501 ataaaggcta tggctgaaca aggtataaca aaaattagat ttactggagg agaacctctt
     4561 ttatatccta aattaaatga gcttataaaa tttacaaaag aagaatgtgg aataaataac
     4621 ataggaatga ctactaatgc tattgggctt tctgatagaa tttttgagct tgaaaaatgt
     4681 ggcttaaata aagttaatat aagtttagat tctttgaagg aatataagta taaatccgta
     4741 acaagaggag gaaacttaaa agatgtctta aaatcgattg aatcttgttt aaacttaggt
     4801 ataaaagtaa agttaaattg cgttgctata agtgggttta atgatgatga gcttaaagat
     4861 tttatgtttt taacagttaa atcaccaatt gatgtaagat ttatagagct tatgccatta
     4921 ggtgaggcta atagagttta taagaagggt tattttaatg taaaagaaat gattgaaaaa
     4981 atatatggtt tatataaagt caaaaatgat gaaaaaagta cagcagaata ttatatgttt
     5041 aaaggagcta aaggaagaat aggaattata actccattaa gctgttcatt ttgtaacact
     5101 tgtaatcgta ttagacttac ttctagtggt gcaataaaat tatgtctaca ttcaaaagaa
     5161 gaaatagata taaaaccatt tttacataaa ccattgttat ttagagaatc tatgaaagat
     5221 ataataaaac aaaaaccaga aaaacatcat ttaatagaaa ataaatgtag tgatacaaat
     5281 aggcgtatgt atgaaattgg aggataagta atgttaacac atataaatga aaaagggtat
     5341 gccaagatgg tggacataag tgaaaaagat gatactaaaa gaacggccgt agctacagct
     5401 acaattactc ttagtaagga agcattagaa aaagtaaaaa atggtcagat aaaaaaagga
     5461 gacgtattgt ctgtagctca agttgcaggt ataatgggag ctaaaaatac atcttcaaac
     5521 ataccaatgt gccatcagat taaattagtt ggatgtgatt tagaatttga tgtagatgat
     5581 tatatgtctg ctattaagat atacgcaacc tgtaaatctc ttgggaaaac tggagtagaa
     5641 atggaagctt taaatgcatg ttctttagca gcacttacaa tttatgatat gtgtaaagca
     5701 gttgataaaa aaatggttat aagtaatatc catcttgtgg agaagactgg tggaaaatca
     5761 ggagatttta agttcgagta aattaataat atattgggtt aaaatttgga ggaaatatgg
     5821 cgaaggtagt gtcaataaat ataagtgaga aaaagggtgt aattaaagtt cctgtaccat
     5881 gtgcagagct aaaaataaat catgggatag ttggagatgc acatgcagga aactggcatc
     5941 gtcagataag cttacttgga aaagaaagca tagataaaat gaaaatgaaa ggctttgacc
     6001 aattaaaata tggagatttt gctgaaaatg taactacaga agggatagaa gttttttccc
     6061 tgcctgttgg tacaaagtta agaatagggc aatgtgaagt tgaagtaact caaataggga
     6121 aaaaatgtca caatggatgt gaaattaaga aattgacagg tgactgtgtg atgccacgag
     6181 aaggaatttt tgtaaaggtt ctaaaagaag gaattataaa tgtgaatgat tcaatagatg
     6241 taatctaatg aagtcactat taaaatataa ttataagtaa atactattaa ga
//
LOCUS       DAELXX010000163         6281 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.165, whole genome shotgun sequence.
ACCESSION   DAELXX010000163 DAELXX010000000
VERSION     DAELXX010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6281
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.165"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(148..363)
                     /locus_tag="KPH29_003428"
     CDS             complement(148..363)
                     /locus_tag="KPH29_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468991.1"
                     /translation="MHINQTGFISNGLGIKQQQKKEPTSNSFSYNAPSSWKPVGRDSV
                     QYNCGFELIATLVREGSSWAFNFIVQP"
     gene            complement(546..890)
                     /locus_tag="KPH29_003429"
     CDS             complement(546..890)
                     /locus_tag="KPH29_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468992.1"
                     /translation="MNYRLKKSVTTILSLLIMTILLPTNASASKVEDSSMKVMYSEKQ
                     VTDMDKLYDMAKNGITDVKSSNEKGIIKNARTGESIYVDSISTTQLLEVKQSKNLTSK
                     IYSKTVFLILIQ"
     gene            1017..1400
                     /locus_tag="KPH29_003430"
     CDS             1017..1400
                     /locus_tag="KPH29_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468993.1"
                     /translation="MRKKFFIGILIVLIISVIAILNIKNDKNVFIGESENWKAKFVTT
                     EGHSKLEIKYKNSKDVVEQKFNYSYQFIDGEDAVVVVKNEILEAGQEKAIKIQRPGLY
                     SNRESSYKLIISWNGNEETINLKKK"
     gene            complement(2609..2860)
                     /locus_tag="KPH29_003431"
     CDS             complement(2609..2860)
                     /locus_tag="KPH29_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468994.1"
                     /translation="MSSYFITRGDVMKILQQFKPYKYDKEKNIEYDEKIIANQNTEVY
                     IVYPRISDEENRRRWEEFNEVAKEVAINIAMKKVETVES"
     gene            complement(3106..3261)
                     /locus_tag="KPH29_003432"
     CDS             complement(3106..3261)
                     /locus_tag="KPH29_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HBG1468995.1"
                     /translation="MALDVKIEVKIIKDVVILNIIHWKKETSLLTVKIARGQGDLSYV
                     KYFTNES"
     repeat_region   4478..5435
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=4478..4506
                     /rpt_unit_seq="ctttacataccacttagttaatataaaac"
BASE COUNT         2242 a          882 c          639 g         2518 t
ORIGIN      
        1 aaattcatat attaagaaat attaatagta aataagcaat tgttactata acgtaggaat
       61 tatctttaaa aacacaaatt aatttacatt ctctaatttt aaagtatatg tatatctttg
      121 attttaataa aattcactta aaatatttta aggttgaaca atgaaattga atgcccaaga
      181 acttccttct cttactaatg ttgcaattaa ttcaaaccca caattgtatt gaactgaatc
      241 tctacctact ggtttccaac tagaaggtgc attatatgag aaagaatttg atgttggctc
      301 tttcttttgt tgctgcttaa ttcctagccc attagaaata aatccagttt gatttatatg
      361 cacccttcta tttgttacac gaatctggcc gtctgcaata gaccagtttc cagaaacttt
      421 actaacggaa atatattgaa atgctccatc tccttttgat attgtatatg taactgtacc
      481 agatgctttt acaccaccag ttttatccca tccatcttct gatatacttg cttttctaag
      541 aggctttatt gtattagtat caaaaaaact gtcttagaat atatctttga tgttaaattt
      601 ttagattgtt taacttctaa taattgagtt gttgatatag aatcaacgta tatggactct
      661 cctgttcttg catttttgat tatacctttt tcattggaac ttttaacatc agtaattcca
      721 tttttagcca tatcgtataa tttgtccatg tctgttactt gtttctctga atacattact
      781 ttcatagatg aatcttctac cttactagca cttgcatttg ttggtaatag tattgtcatt
      841 atgagtaaag ataaaatagt tgttacagat ttttttaacc tataattcat attaatcctt
      901 actttttatt tgttataaca gacattctat tgtaatagaa tataaatcca caatattttt
      961 aaaattagta ttgaataact attctcatgt tataattatt caaaggaggg attgatatga
     1021 ggaaaaaatt ttttataggt attcttatag ttttaataat ctctgtgata gctatactta
     1081 atataaaaaa tgataaaaat gtctttattg gagaatcaga aaattggaaa gctaaatttg
     1141 taactactga aggacactca aaattagaaa taaaatataa aaatagtaaa gatgtagttg
     1201 aacaaaaatt taattattcc tatcaattta tagatggtga agatgcagtt gtagttgtaa
     1261 aaaatgaaat tttagaagca ggtcaagaaa aagcaataaa aatacaaaga ccaggtcttt
     1321 attcaaatag agaatctagt tacaagctaa ttatatcttg gaatggaaat gaggaaacta
     1381 taaacttgaa aaagaaataa ccatattatc aatttttaaa aaattattat tcaaattctt
     1441 ataaaaagag tgtttagaaa aatgaaacac tcttttttgt ataagttttt gaaaactata
     1501 aatgaaatat atcaaaatcg ttcgaaaaat tactgaaata aactcttaaa tatattataa
     1561 attaaggtat attatacatg aatttattgg gagtttatga gttttaaaat tggaaaccaa
     1621 acaataaggg ctaaaaatac tttaaggaaa atatataaag ttctataaat tatcaaaaat
     1681 atttcgcaaa aattgataat tattacaaat aaattgataa tactaataat attagaaata
     1741 tcgaaattga acaaactagg attaaagctc aattagaaaa atccactaat cctgagaatg
     1801 ctttgttatt ctcatttatt actgttgttt tttcattatt tgctaattta tttttcagtt
     1861 cattaaatgt taaatataac tctataaatt taattttaca gttaatatgt attatagttc
     1921 ttatatttat atatattaat acatttttat atcgcctctt caatttgagc cccatcatta
     1981 tataaaatta acctacttga tatcttaatt taatatatgt acactattca tatcttaata
     2041 gtcaatatta ttcattttta atagctcttt ttagatgtca gaattattat attttgaaat
     2101 ttattaaatt tttgcattaa aaaaactaga aattaattta agtcttgaat tacttataac
     2161 tttctacgta tctaccttta tattcttact ctgattttat atctaatatt aactcttcat
     2221 caaagctatt aaatatacct ctaattgagt caattatttc acttacttca cctttaattt
     2281 ttatattaac ttcaaccatt tcaaatctca tctttttatc ttataatttc tataagtttt
     2341 tttatttatt gtgttgctta ctttgaccag cactttttta tttaatagtc taaatgatat
     2401 tttttaccag ttttaatatc cttaaatact atatttacta aaaacttata atttctttta
     2461 atacattctt attactaata acaatactca acaattttat cgcctttttt caaatatgat
     2521 ttaacttttt tattcctaat tatttaaaaa tagttgtcca attttcttct atgagaaagt
     2581 gctaagtatt agcaacttct ctattctctt aactttctac tgtctcaact ttcttcatag
     2641 ctatattaat agctacttct ttagctactt cattgaactc ttcccatctt ctcctatttt
     2701 cttcatcaga tatacgtgga tatactatat aaacttctgt gttttgatta gctataattt
     2761 tttcatcata ttctatattt ttttctttat catatttgta tggtttaaat tgttgtagta
     2821 ttttcataac atcacccctt gttataaaat atgaactcat aaacttgtct tatacttatt
     2881 taattgttac aatttttact taaaatcact ttatattcta tattttaaag ctaaatcctt
     2941 tacaaaagta acatattctt ctataagttt cttatcctct tatatagtat ctagtttgtt
     3001 taacttatct cttttaaact tacatacgcc ttcttctgcc attcgtcttt tattaattgt
     3061 taacattgtt tcaagagaca caccaaaact cttgttaaga agctcttagc tctcattagt
     3121 aaaatatttt acataactta aatcaccttg acctctagca attttaactg ttaatagact
     3181 tgtttctttt ttccaatgta ttatatttaa tataacaaca tcttttatta ttttaacttc
     3241 tatttttaca tctaatgcca tattattgct ttattaattt gtatccttaa atcttaaatt
     3301 ttctattaat acatcttcta tacagcttag tttctctcgc ttaatcttga aatatatttt
     3361 ctctaaattg tcaaattgtt cccaagtttt atcaatatct aatattttgc agtgtctatt
     3421 tgcacatctt tcgatccaaa ggtataattg actagaatgt tttgaaatca actgactatt
     3481 agtaggttag ttcttaaatt ctttcaaata ctcgaattgt aataaaacat aatttacatt
     3541 ctctttattt ctattgaagt tagtttatat attgtttaca tcattttcat atatatctgc
     3601 tagttgctat gcatttaaaa ttctcttatt atttctttat attacttgta aattattcat
     3661 cctcatcatc ctcttaatta aatttctatc ttagaaattt aatttgtaaa aaaattttct
     3721 atatcataat caagtatctt agatagttta taggtaacat atcaactcta attgaatatt
     3781 accccttcct gtacttaaaa taagttgatg catttttaaa acctaactct tttgcaacac
     3841 tttgtaagta taaattttat gtatgtcttc ttttttataa aatcatagtg tatctttttc
     3901 atatattcac cttaattgct atatttcaat aacaatattt ccaaaacaga aatttttcga
     3961 ggtaattatg tatagatgta aatcaaagaa ttaaatatac tagggaaaat atgaatagtt
     4021 ctcaaaaaga attgcttgaa aaaacaaata taaatacaag tgttatgaat agaatctggt
     4081 gaaagaccaa caagagacga tgggttaata atatttgcta aaatttttgg tttatatact
     4141 gattatgttc ttggtttatc agatacagaa aaactaaata ttggcaatta aaactgaatt
     4201 gattgtatta tatgtgtgat gaatataaat atagaatttt ttgagaagct tgaaatagct
     4261 tagaaaaaat aagagattca ctcaagaata agttactaaa gattaaaaaa taacaaaagt
     4321 atctactaaa ggatatgaaa atggtttaag agagttaagt ctggaaatat ttatgtgtat
     4381 tctttaaata aaaatttatt ttttaaatga taattaaatt tttactgata tggctatgct
     4441 tttaaaagtc ataaccttat tttgatatta gatctgtctt tacataccac ttagttaata
     4501 taaaactaaa tatagcttgt aactcctttg atgcacttaa aagctttaca taccacttag
     4561 ttaatataaa acgaatttag ctatagaagc atcaaattgt ggcacagcac tttacatacc
     4621 acttagttaa tataaaacct ttaaagacaa aacgtcgtat aattaaatca tagcaacttt
     4681 acataccact tagttaatat aaaaccttat aattctttaa gtactagact gagcaattta
     4741 atctttacat accacttagt taatataaaa ccatcccatt cagcaacaac agtcgctaaa
     4801 tcttctgcct ttacatacca cttagttaat ataaaacttt tagtatattt tttaggtctg
     4861 ccctctttaa aactctttac ataccactta gttaatataa aacacgaggt tatagttaat
     4921 tttgtcgtgg gagtttataa ctttacatac cacttagtta atataaaaca atttatatat
     4981 atatatatat atattaaagt cagacccctt tacataccac ttagttaata taaaacttgt
     5041 tgttcagcat tagcaatcat atcatctatt cctttcttta cataccactt agttaatata
     5101 aaacctgtac catcagagac agtcattaaa agatttataa actttacata ccacttagtt
     5161 aatataaaac ttataaattg tctctgtacc cattttattc aatgtcgtac tttacatacc
     5221 acttagttaa tataaaacca tgaaaaaatg ggtaattcaa actcttttat tttctttaca
     5281 taccacttag ttaatataaa acgttggaga aaataatcct tcttatgatt tgccaagcgg
     5341 ctttacatac cacttagtta atataaaacc agcggatgaa ttatttatgt taattggaga
     5401 tggagacttt acataccact tagttaatat aaaaccccaa aataaattga gtatttccaa
     5461 cacttacatc tatacaattc tctcaaattt gcagtgaacc atgagtagtg caaatgataa
     5521 catttatcac ataccctcaa tgccttatat ttcaactgtt aggccctatt ttatcaaaaa
     5581 tatcgctcac tgcaaaacta tttataattt tataatatta tgaatatatt atttttaaat
     5641 atctgtacca atttgtagta taataaaatc aagaaaataa tttactccta taaagagtga
     5701 ttatttttaa gtgaaaatat tattttttta aatcatcctt attggcgttt gggagatttt
     5761 tattttatca taaatataaa ctgatataac accagctgac atgcttgctg ctttcgatta
     5821 ttattcttgc tacaattatt ataacatata aaacttactt atttttacca aatattttat
     5881 acaaacaata aatattaact tatgaaatat ctactcattt acagatattt cataagttaa
     5941 tagctctata ataaaatttt tatcattgtt tataaaaaat tttactttgc atttttttat
     6001 aaacaacagt tttatttata atgttttgtg gtataataaa agcaagaaga actacaatct
     6061 attttgcgtt agagtggagt tcatcataaa cagagtttat tttttggatt taaatataaa
     6121 tttaaattca actcgtaagt cactctttgt gctgaactat acccactgtt ttatacagtt
     6181 aaaacatgta aaagtggtta taaagtacta gtgtcaatca ttagtacttt ttttattata
     6241 ttcatataat taatagctat attttaaagt ttactatcta a
//
LOCUS       DAELXX010000164         6157 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.166, whole genome shotgun sequence.
ACCESSION   DAELXX010000164 DAELXX010000000
VERSION     DAELXX010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6157
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.166"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            692..2101
                     /locus_tag="KPH29_003433"
     CDS             692..2101
                     /locus_tag="KPH29_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HBG1468996.1"
                     /translation="MIFSSLIFLFLFFPVTLICYYLANPKYKNVMLLLASIAFYAWGE
                     PKYIFLMILSIIVNYIFGIKVSGSKNKKMWVVLSVIYNISILAVFKYSNFFIDNINFL
                     FNTDITIPEVKLPLGISFFTFQIMSYVIDVYRNDAKVQRKLANLALYISLFPQLVAGP
                     IVRYQTVADSIEYREHNWDKFTQGVNRFVVGLGKKVILANQLGVIADGVFNKSAGDLS
                     RLEVWLGIVCYTLQIFYDFSGYSDMAIGLGKMFGFEFLENFNYPYISQTVSEFWRRWH
                     ISLGTWFKDYVYFPLGGSRVSKAKLYRNLFVVWFLTGMWHGASWTFIIWGLYYGILIS
                     VEKAFLEKFLYKMPRIVRHIYLLLIVMIGWVFFRVDDLPAAIKFIGIMFGAGNYPVIS
                     DAFNVFIIEYWYIIVGAILLSTPIVKVVKSLLIKKNKNTLNSTLYQVTNVVFIVSCMF
                     IVIIMLSSSTYNPFLYFRF"
     gene            2117..3319
                     /locus_tag="KPH29_003434"
     CDS             2117..3319
                     /locus_tag="KPH29_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468997.1"
                     /translation="MSKKKPRIVAISFLIIVFGFFILNFTLPKKKLSITENRNLAKFP
                     TVENLKKCDFIAKFEEYFNDHFAFRDEMISINTRTQANLNKTKVGNYYLGKDNWILGM
                     FPEILDQKQLSRYEKSINYLSNLSKDMGKNVYFTMMPHKTNMLKHLYPKYVDNMDNID
                     INLKNFKNRLNPDLIKYIDMDEYFLSNYSDKERENLYFKTDHHWTGYGAFEGFKMMAS
                     NMDLGLSKQDLDKHFSSYKKSIITDKKFIGSYNQNIDMLIKENEYVVYMTKDNQDYSF
                     DINGEEKEEKEEKEVYATERDNKEWDYGGAYIRGAKTNILTIKNPKALVDKKILIFRD
                     SYQAPTTLLFADMFKEVEIADPRNINSIYKTYKEIIEESNADIVMFMYNSSGFDSMID
                     SMIDKGIK"
     gene            3520..4503
                     /locus_tag="KPH29_003435"
     CDS             3520..4503
                     /locus_tag="KPH29_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468998.1"
                     /translation="MKEVFNVHIMQSQNKKIKQKYYLTKDGWLLGNFAKLLSDYHIKS
                     YSKAINNLSKLNRKKDFYFVSLPHKTNMLKHLYPSGIEGKNNIDINKENLEKQLTDIN
                     FLDIDDYFYRSFTEKEREKFFLKTDHHGNSLGAFEEYKFILDKLDLGLSKSEIDSHLK
                     RYSTRYVNDKVFIGSYNKKMGYPLKKKESISYVEISDANYKYFISNGTKNTEINENII
                     KAKLKDKREWDYGGAYMRGCDCHILKIRNSSSLTNKKALIFRDSYQAPMTWMLADTFK
                     ELEILDPRYIKNIDMTYDEIIKNSNANVVLFMYNSFGFAGMIQEMIDKGIA"
     gene            complement(4953..5396)
                     /locus_tag="KPH29_003436"
     CDS             complement(4953..5396)
                     /locus_tag="KPH29_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1468999.1"
                     /translation="MKKNMLYLGIVYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GICMIFQYMYWSKPERAVDYEEKIKNQRIEMNDERRVMLRDKAGRITNQIMSYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKKL"
     gene            complement(5555..>6157)
                     /locus_tag="KPH29_003437"
     CDS             complement(5555..>6157)
                     /locus_tag="KPH29_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG1469000.1"
                     /translation="EAFENTNNQIGLDLGIKEFCISSCGEFIENPKYFKKSLNKLAKL
                     QRELSRKTIGSLNRNKARLKVAGLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVK
                     NMIKNRKLSRLISDVSWSEFIHQLKYKANWYGRQIVKVGKFFASSQICNKCGYKNEEV
                     KDLNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
BASE COUNT         2392 a          765 c          826 g         2174 t
ORIGIN      
        1 agaaaaaata aagaaaaaaa gtaaccctta gttgtaaaag ggttattttt ttgtgataaa
       61 atatccataa aaatattcat accaaagtaa attaagatgt atttcatttc tcaaaaatat
      121 cctaatataa aaaataaaga aatatttata aatttaatta ttagacatag ataatatcta
      181 aaaaatatac caatttcaac aaatagtaac ttttttacaa caaatggaaa ctatggaagc
      241 aataattaaa ataaaattta taatcaatca taattattta gtataatgat tcgatattat
      301 tttaaagtat ttgtcattta aaacaaactt gcgataaaag catatctgaa agtattctga
      361 ttctaatact taataaagag ttctttttac agttatagat ataaacaaca tctatcttaa
      421 tagactgtat agaggcacaa tcaaaggtaa acacatatag ttaagtaata tgtccttgat
      481 ttttctttat tataatagct ttattaattc attttaatat tacatcattt taaaaaaatt
      541 atatgaaaag tcaaaattta catatagatt aattaaactt gtaaaaacac atataaattc
      601 atacaattaa gtacttaaaa cttgaaaata cttactttgc tatatatttt agataaagat
      661 ataattgatt tggttaagaa aggagtctaa tatgatattc agtagtttaa tatttttatt
      721 tttattcttt ccagtcacac tcatttgcta ttacttggca aatcctaagt ataaaaatgt
      781 tatgttactt ctagcaagta ttgcattcta cgcatgggga gaaccaaaat atattttttt
      841 gatgatacta tcaataatag taaactatat atttggtatt aaagtttctg gcagtaaaaa
      901 taaaaaaatg tgggttgtgc tttctgttat ttacaatata tctattcttg ctgtatttaa
      961 gtatagcaat ttttttatag ataatattaa ttttttattc aatacagata taactatacc
     1021 tgaggttaaa ttacctttag gtatctcatt tttcacattc caaataatga gctatgtaat
     1081 agatgtatat agaaatgatg ctaaagttca aagaaaatta gctaatttag cattatacat
     1141 aagtttattc cctcagcttg tagcaggtcc tattgttaga tatcagactg ttgcagatag
     1201 cattgagtat agagaacaca attgggacaa atttactcaa ggtgtaaaca gatttgttgt
     1261 tggtcttgga aaaaaagtaa ttctagcaaa tcaattaggt gtaatcgcag atggtgtatt
     1321 taataagtca gctggagatt tatcaagact tgaggtatgg ctaggtatag tttgctatac
     1381 acttcaaata ttctatgact ttagtggcta tagtgatatg gccattggtt taggtaagat
     1441 gtttggattt gagtttttag aaaatttcaa ctatccatat atctctcaaa cagtatctga
     1501 gttttggaga agatggcata tatctttagg tacttggttt aaagactatg tgtacttccc
     1561 attaggtgga agtagagtct ctaaggcaaa actatataga aatctatttg tagtatggtt
     1621 tttaaccggg atgtggcatg gagctagttg gacttttatt atatggggtc tatattatgg
     1681 aatattaata tcagtagaaa aagcattctt agaaaaattt ctatataaga tgccaagaat
     1741 tgtaagacac atatatctac ttcttatagt catgattgga tgggtatttt ttagagttga
     1801 tgatttacca gcagctatta aatttatagg tattatgttt ggtgctggaa attatccggt
     1861 tatcagtgat gcattcaatg tatttataat tgaatattgg tatataattg ttggggcaat
     1921 ccttctatca actcctattg taaaagttgt aaaatcatta ttaattaaga aaaataaaaa
     1981 tacattaaat agcacattat atcaagttac taatgtggtt ttcattgttt cttgtatgtt
     2041 tattgtaata ataatgttaa gtagctcaac ctataatcca ttcttatatt ttagattcta
     2101 attgggaggt ataaacatga gtaaaaaaaa accaaggatt gttgccattt catttttaat
     2161 tatagtattt ggatttttca tactaaactt cacacttcca aagaaaaaat taagtataac
     2221 tgaaaataga aatcttgcaa aatttccgac tgtagaaaat ttaaaaaaat gtgactttat
     2281 agccaagttt gaagaatatt ttaatgacca ttttgcattt agagatgaaa tgatatcaat
     2341 aaatacaagg actcaggcta atttaaataa aactaaggta ggtaactatt atctaggaaa
     2401 agataactgg atattaggta tgtttccaga aattttagac caaaaacaat taagtcgata
     2461 cgaaaaatcc attaattatt tatctaatct aagtaaagat atgggaaaaa atgtgtattt
     2521 tacaatgatg ccacataaaa ctaatatgtt aaaacatttg tatcctaaat atgttgataa
     2581 tatggataat atagacataa atttaaagaa ttttaaaaat agattaaatc cagatttaat
     2641 taaatatata gatatggatg agtatttttt aagtaactat tcagataaag aaagagaaaa
     2701 tttatatttt aaaactgacc atcattggac tggatatgga gcatttgaag gctttaaaat
     2761 gatggcatca aatatggact taggtctatc taagcaagat ttagacaagc attttagtag
     2821 ctataaaaag tctataatta ctgataagaa atttatagga agttataatc aaaatataga
     2881 tatgctaatt aaagaaaatg aatatgtagt ttatatgaca aaagataacc aagattatag
     2941 ctttgatata aatggagaag aaaaagaaga aaaagaagaa aaagaagttt atgcaactga
     3001 aagagataat aaagagtggg attatggtgg tgcatacatt agaggagcta aaactaatat
     3061 attaactata aaaaacccta aagcattagt agataaaaaa atactaatat ttagagattc
     3121 atatcaggct cctacaacac ttttatttgc tgatatgttt aaagaagtag agatagcaga
     3181 ccctagaaat ataaactcaa tatataaaac atacaaagag ataatcgagg aaagtaatgc
     3241 agatattgta atgtttatgt ataatagttc tgggtttgat tcaatgatag attcaatgat
     3301 agataaagga ataaagtagt aaataaaaag cctctattaa attcaaaatt taatagaggc
     3361 tttttattgt ttatttttta ttgctttggt aaaaataaac taatcaaaat aaaagtaagg
     3421 agatgtagca tattgaacaa aagacatagt ctacctatag tatcagtgat attattgatg
     3481 gtcttaatta tctttaataa taaccaaaat tatattacaa tgaaagaagt ttttaatgtc
     3541 catattatgc agagtcaaaa taaaaagatt aaacaaaagt attatctaac taaagatgga
     3601 tggctattag gcaactttgc taaattatta tctgattatc atataaaatc ttactccaaa
     3661 gccattaata acttgagtaa gttaaataga aaaaaagatt tttactttgt ttcacttcct
     3721 cataagacaa atatgcttaa gcacttgtat ccaagtggta tagaaggtaa aaataatata
     3781 gatattaaca aagaaaactt agaaaagcag ttaacagaca taaatttctt agatatagat
     3841 gattactttt atagaagttt taccgaaaaa gagagagaaa aattcttttt aaaaacagac
     3901 catcacggaa attcattagg agcatttgaa gaatataagt ttattcttga taaattagac
     3961 cttggcttat ctaaatcaga aatagatagt catttaaaaa gatacagtac tcgatatgta
     4021 aatgacaagg tctttatagg tagttataat aaaaagatgg gatatccact taagaaaaaa
     4081 gagtctataa gctatgttga aataagtgat gcaaattaca aatattttat tagtaatggt
     4141 actaaaaata ctgagattaa tgaaaatatc ataaaagcaa aattaaaaga taaaagagaa
     4201 tgggactatg gtggagctta tatgagaggt tgtgattgtc acatacttaa gattagaaat
     4261 tcaagctctt taactaataa aaaggccctt atatttagag attcgtacca agcacctatg
     4321 acttggatgt tggctgatac ttttaaggaa ctcgaaatac tagatcctag atatataaaa
     4381 aatatagata tgacctatga tgagataatt aaaaacagta atgcgaatgt agttttattt
     4441 atgtataata gctttggatt tgctggaatg atacaagaaa tgattgacaa gggaatagca
     4501 taacaatttg aatcagctcc tttttataca aaaaatagtt ctcaataatt cctaatctat
     4561 atgataattt agtacatctc tttaacaata aaaataaaat cacaaaaatt cttataccat
     4621 cacttatctt tatgataaat tggttgagaa aaaattttat aatttatatg atatgcaatc
     4681 ttaattaatt ctttatctac tgcaaccttt aataaaagaa accatccaag actattaata
     4741 tgaatttata taaaaattac tttcaaataa agtactgcag tatttttatt taaagtatct
     4801 ataatcttta accacaaaaa gccacaaaat acaatgtata cttatggctt tttataaatt
     4861 tatataattg tatataaaga tatattctaa aactgtctta agaatctaaa gtttaaaaat
     4921 ccaatatttt tattgtgaat ttaaactttt tattacaact ttttatctaa tttattgtat
     4981 acaacaacac tacaaataaa ttgaaataaa ataagcaatc caagaacaac taaaacccac
     5041 ttgcttatta ccattacaga aaatacactc aatatagaaa caataaatat aagaattaca
     5101 agaacatagg acatgatttg attagtgata cgtccagctt tatcacgtaa cataactctt
     5161 ctttcatcat tcatctctat cctttggttt tttatctttt cttcataatc tactgctctt
     5221 tcaggcttac tccaatacat atattgaaaa atcatacata tacctggacc taaagcagct
     5281 ccagataatc cccaaataaa agcctcaaat gaaaactcag taaacgtagc caataaaaca
     5341 agtaaaattc cacaaataaa atatactatt ccaagatata acatattctt tttcattttt
     5401 actcctcctc ataaataaat atttcttcaa tactcatctg aacctcccat gactaaagtc
     5461 acagggttcc tgcttcacag aattttgcat actaaaatat gtcttactaa ttctccacag
     5521 gccatccccg tagttcctac ggttcttaca tacgttattt attttgaatt gttattaatc
     5581 ttaatccttc ttttaatata tttatacttg catttatatc tctatcatga gtttcattac
     5641 aactaggaca aatccattct cttatattta agtccttaac ttcctcgttt ttatacccac
     5701 atttattgca tatttgtgaa cttgcaaaga atttgcctac ttttacaatt tgtctaccat
     5761 accaattagc cttgtatttt aattgatgaa taaattctga ccatgataca tcagaaatta
     5821 agcgagatag tttacgattt ttaatcatat tctttacttg taaatcttct atgcaaataa
     5881 tatcattctc ttttattaat ttagtagata gtttttgtag aaaatcattt ctttgattag
     5941 ctatatgttc ttgaagtcct gcaactttta atcttgcttt atttctattt aaactaccga
     6001 ttgtttttct cgataattct ctttgtaatt tagcaagttt gtttaatgac ttcttaaaat
     6061 acttaggatt ttctataaat tctccacaac ttgagataca aaattcttta attcccaaat
     6121 ctaaacctat ttgattgtta gtattttcaa atgcttc
//
LOCUS       DAELXX010000165         6100 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.167, whole genome shotgun sequence.
ACCESSION   DAELXX010000165 DAELXX010000000
VERSION     DAELXX010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6100
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.167"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(19..1206)
                     /locus_tag="KPH29_003438"
     CDS             complement(19..1206)
                     /locus_tag="KPH29_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG1469001.1"
                     /translation="MNITKNFLKDYLEKFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNARIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRVVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKSFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
     gene            1479..2303
                     /locus_tag="KPH29_003439"
     CDS             1479..2303
                     /locus_tag="KPH29_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBG1469002.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHRQEVREAIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVCKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GNNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            2432..3697
                     /gene="gluD"
                     /locus_tag="KPH29_003440"
     CDS             2432..3697
                     /gene="gluD"
                     /locus_tag="KPH29_003440"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HBG1469003.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            3890..4546
                     /locus_tag="KPH29_003441"
     CDS             3890..4546
                     /locus_tag="KPH29_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG1469004.1"
                     /translation="MYFLPPHDYKLEELDWIFGHELMYFKSKDLHLKYLVLFLKIVYW
                     FNPFIYIMEKYMDLDCELYCDERVLKNCTVQQKQEYALTIVNSMKRGINFSNKFVTGL
                     NKQSDIRKRVMNMFSEKHKNGILMALIISLFSSITFFKFDSTSLIEENDFLERSRYAF
                     EGRQEDMFVTIDSTYADAPIKLRKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMDLKK
                     "
     gene            4877..6010
                     /locus_tag="KPH29_003442"
     CDS             4877..6010
                     /locus_tag="KPH29_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HBG1469005.1"
                     /translation="MSNFILNGIFRTVIVSSLSILLILIFKKNVFKRFSKKFNYYIWM
                     IVVIKLFLPFTYYTFTINILRSKKHININNINLEGFNNVSTISNNIILYTWIITVVVY
                     LIYTIFKYIKLKNLINDLSYDVDDREIVNLYKNILEEFNITKDIKLKYSYEVETPAFF
                     NSCVLLPPHEYKLKELDWIFRHELMHFKSRDLYLKYVILFLKTVYWFNPFIYIMDKHI
                     DLDCELYCDERVLKNRNSEEKKDYALTILNAMRKGSNTSNKFIAGLHKQSDIKKRVMN
                     MFNEKYKNGILMALTLCLLSSITFLKVNSTSIVNLNVLPKGFEDLRTVSSIRFSVIGF
                     TYADAPKNMREEYKINCESLGLIPKDSDKIEVGVEYYDSLLND"
BASE COUNT         2346 a          668 c          981 g         2105 t
ORIGIN      
        1 tattcaattt gtaaaatatt attttgaaca attatacata tactctctag ttagaggtaa
       61 atcatttttt atacctttag tgaaaagtat ttgatgtata tctataatac cagtgttgaa
      121 agcagcagca caagaacaaa ggtataaatc ccacattctt ataaacttat catcaaacat
      181 ttctttaatc tttggaagat tctcagagaa acttttttgc cagtatagta aggtttttac
      241 ataatgtaat cttaaacttt ctacgtctat agtatgatat ttgtaatcac tacaaatatg
      301 tatcatttct cttaggctag gaataactcc tcctgggaaa atatattttt ttatccaagg
      361 gtctccataa gattcctgca atccacttat atagtgaagt aaaaacaatc caccatcttt
      421 taaaacagca tttacgtttt taatgaaaag ttcatagttt tctctaccaa catgttcaag
      481 cattccaacg cttacaactc tatcaaacat aaggtctgat ttttttaatt ctctatagtc
      541 cataagtctt acctcaagat agtcttgaag gttctcttct ttaattcttg cgttaaattc
      601 cttaaattgc tcttcgctaa gtgtaattcc aacgccgtga attttatatt tttttgcagc
      661 ttctattagt aaaaatcccc aaccacaacc aatatcgaga aggctcatac cttcctctaa
      721 atttagttta gttaatatgt ggtgaacttt attgacttga gcatcataaa gagtatcatt
      781 atcatttcta aagtatgcac aagaataact aagtgtctta tctaaccata agctataaaa
      841 atcgttgcca atgtcatagt gggcatgtat ttctttcttc tgatgtctca ttgaatttga
      901 tgagtgcaat agatgtttta cagctgaatt atcaatattg aatttgtcta tttgttttaa
      961 aagtagattt aaagttaaga ataaatctcc ctcaacttca atgtctctat ttatgtaggc
     1021 ttctccaagt gctaaagatg tactttttaa tagttcagac ttgctaatat cattatttac
     1081 tataactttg aactgagggg agccctctcc aattagaaat tcttcctcat ctttaagcct
     1141 aactagaaat ggtgtttcac caaatttctc taaatagtct tttaaaaaat tttttgtaat
     1201 attcatatta ccagctcctt ctaatgtaaa tccagtaagt ttccctgctt aaaatatatt
     1261 atagtccttt ttttaaaaaa gtaaaatttt ttcaataaat tttttattta aaggaaattt
     1321 attttttata aagaaatttc atgttgtagt atattttttg cttataaatt ttggtactgg
     1381 atataatatt aaactgttaa tgatataata aatttaatct ataaagtaaa taaaattttg
     1441 taataaatag ttaaagctat tttataagga gatatatgat ggaagtatat gctttggttg
     1501 ggtcaagtgg cacagggaag agctttaagg ctttagagtt cgcatatgaa aatgatatag
     1561 aatatataat cgatgatggg attttaatat acaaaaacaa ggttttagct gggatatcgg
     1621 caaaacaagc aaatactact atagaagcag ttaaaagggc tattttttat aatctggagc
     1681 atagacaaga agttagagaa gcaattaaaa aagaaaatat aagaaaaatt cttattattg
     1741 ggacatcaaa aaaaatggtt aatcaaattg tagagagatt gtcaattgga agggtctgta
     1801 agtttataaa tatacaagat ataagtacta aatatgaaat agaaatagca aaacaagcta
     1861 gaaaagaagg aaatcatgta attcctgttc cagccgttga gataaaaagt atggcaagtg
     1921 gtctcagtat aaattctttg aagagacttt ttagaaaagg aaataatagg aatatggtag
     1981 ttttagaaaa aacaattata agaccaactt ttagttatat aggaaagttt tatattagtg
     2041 ctgatgtaat taaacagatt attgaatatg aagtatacaa ctttggaagc attgatagaa
     2101 taaataaaat caatatagag aattgcaata attttatgaa tatttttgta agtataaata
     2161 tcaatgattt agaaactata aaaaaaattg aagacataca acagacaata aaaaagtcta
     2221 ttgagaaaat gactttagtt aatgtacaaa aaatagatat taacattcat aaaattaaaa
     2281 aaataaactt cctccaatgt taaaaaataa actgagaaaa tgatatacta atttgcaata
     2341 aagtgtgcta taattaaact gtaaatttag gaaatggttt tttctaaatg gagtatacat
     2401 aattataacg tatttataag ggggctttca aatgtcagga aaagatgtaa atgtcttcga
     2461 gatggcgcaa tctcaagtaa aaaatgcatg tgataaatta ggtatggaac cagcagttta
     2521 tgaattatta aaagaaccta tgagagttat agaggtttca attccagtaa aaatggatga
     2581 tggttctata aaaactttta aaggatttag atcacaacat aatgatgcag tagggccaac
     2641 aaaaggtgga ataagattcc atcaaaatgt ttcaagagac gaagtaaaag ctttatctat
     2701 atggatgact ttcaagtgtt ctgtaacagg tataccatat ggtggaggta aaggtggaat
     2761 aatagtagat ccatcaactt tatctcaagg tgaattagaa agattaagta gaggatacat
     2821 agatggaata tataaattaa taggtgaaaa agttgacgtt cctgcaccag acgtaaatac
     2881 taatggtcaa ataatgtctt ggatggttga tgagtacaat aaattaactg gacaaagttc
     2941 tataggtgtt ataactggta agccagttga atttggtggt tctttaggaa gaacagctgc
     3001 tactggattt ggtgttgctg ttactgcaag agaagctgct gctaaattag gaatagacat
     3061 gaaaaaagct aaaatagctg ttcaaggtat aggaaacgtt ggttcttata cagttcttaa
     3121 ctgtgaaaaa cttggtggta ctgttgtagc tatggctgaa tggtgtaaat cagaaggttc
     3181 ttatgctata tacaatgaaa atggtttaga tggtcaagct atgttagatt atatgaaaga
     3241 acatggtaac ttattaaact tcccaggagc taagagaata tctttagaag agttctgggc
     3301 ttcagatgtt gatatagtaa taccagctgc attagaaaac tctataacta aagaagttgc
     3361 tgaatctata aaagctaaat tagtttgtga ggctgctaat ggaccaacta ctccagaggc
     3421 tgacgaagta tttgctgaaa gaggaatagt tcttactcca gatatattaa ctaatgctgg
     3481 tggagttaca gtttcttact tcgagtgggt acaaaactta tatggatact actggtcaga
     3541 agaagaagta gagcaaaaag aagaaatagc tatggttaaa gcatttgaat ctatttggaa
     3601 aatcaaagaa gagtacaatg ttacaatgag agaagctgct tacatgcatt caattaagaa
     3661 agttgctgaa gctatgaaat taagaggatg gtactaagat ttactcttaa taaataatag
     3721 gatataatta tttaagatat aaaggggctg tacatagtac agcccttaaa ttatgtggct
     3781 tacaatgaag taagctattt ttgttttaac ttataaataa cttgataaat tatgtaattt
     3841 agaatgcata aataagatgg gtgaaaatta atggttgatt taattttgaa tgtatttttt
     3901 accaccacat gattacaaat tagaggagtt agattggatt tttggacatg aacttatgta
     3961 ttttaaaagc aaagaccttc atttaaagta tctagtatta tttttaaaaa tagtatattg
     4021 gtttaatcca tttatatata taatggaaaa atatatggat ttggattgtg aactttattg
     4081 tgatgagaga gttttaaaaa attgtactgt acaacaaaag caagaatatg ctctaactat
     4141 tgtaaattca atgaaaagag gtataaattt ttcaaataaa tttgtaactg gattaaataa
     4201 acaatctgat attagaaaaa gagtaatgaa catgttcagt gaaaaacata aaaatggtat
     4261 cttaatggct ttgattataa gcttgttttc ttctattaca ttttttaaat tcgattcaac
     4321 tagtcttatt gaagagaatg atttcttaga gaggtcaaga tatgcgtttg aaggaaggca
     4381 agaagacatg tttgtaacaa tagattctac atatgcagat gcacctataa aacttagaaa
     4441 agagcatgaa gaaaactgta gagtcaatgg aatagtacca aaagattcag attcaataga
     4501 aattattcct agattttata aagattgtat ggatttaaaa aaataagtta gacttaatat
     4561 tgatgttgat attatgaatt ttttatattt aagcaattga aaattttatt atagattcaa
     4621 ttttatatgt gtttaagtat gaaatttata gatagttgaa ataattaaat atgattgatg
     4681 cataggaatc attactaagc tataattaat tataggggag tttaaataaa ttatgagtta
     4741 cacaattata aaatgataaa aaatagttat ctgttacaaa aaatgtatct ttttaattaa
     4801 taaagataat atgaaaactt aaaaggcaaa taaacttaaa cataaaatac tcttaaataa
     4861 gataggagaa aaatgtatgt ctaactttat tctaaatggg atttttagaa cagttatagt
     4921 aagtagtcta agcattcttc tgatattgat tttcaagaaa aatgtattta aaagatttag
     4981 caaaaaattt aattattata tatggatgat agttgttatt aaattatttt taccatttac
     5041 atattatact tttacaataa atatcttgag aagtaaaaaa cacattaata ttaataacat
     5101 aaatttagag ggatttaata atgtttcaac tataagtaat aatattatat tgtacacttg
     5161 gattataact gtagtagtgt atttaattta cactatattt aaatatataa aacttaaaaa
     5221 tttaataaat gacttatctt atgatgttga tgatagagag attgtaaatc tttataaaaa
     5281 tatattagaa gaatttaata taacaaaaga tataaaactc aaatactctt atgaagttga
     5341 aacaccagca ttttttaatt catgtgtact tttgccacca catgagtata aattgaaaga
     5401 gttagactgg atttttagac atgagcttat gcattttaag agtagggacc tttatttaaa
     5461 atatgtaatc ctgtttttaa aaacagtata ttggtttaat ccatttatat atataatgga
     5521 taaacatata gatttagatt gtgaacttta ttgtgatgaa agagttttaa aaaatcgaaa
     5581 ttcagaggaa aaaaaggatt atgctttaac aattctaaat gcaatgagaa aaggttcaaa
     5641 tacttcaaat aaatttatag ctggactcca taagcaatct gatattaaaa agagagttat
     5701 gaatatgttt aatgaaaagt acaagaatgg tatattaatg gcattaactt tatgtttatt
     5761 atcttctatt acttttttga aggttaattc aacgagtatt gttaatctta atgtattacc
     5821 taaaggattt gaagacttac gaactgttag cagtatacgt ttttcagtta taggatttac
     5881 atatgcagat gctcctaaaa atatgaggga agaatataaa ataaattgtg aatcattagg
     5941 tttgatacct aaagactctg ataaaataga agttggtgta gaatattatg acagtctatt
     6001 gaatgattaa ataaatatta ttttaacaga tattaattat aaagttttga atagtaattt
     6061 gttgagaaat aaatatatcc ttataaaaat agatacttat
//
LOCUS       DAELXX010000166         6086 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.168, whole genome shotgun sequence.
ACCESSION   DAELXX010000166 DAELXX010000000
VERSION     DAELXX010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6086
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.168"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(86..2362)
                     /gene="slpA"
                     /locus_tag="KPH29_003443"
     CDS             complement(86..2362)
                     /gene="slpA"
                     /locus_tag="KPH29_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HBG1469006.1"
                     /translation="MNKKNIAIAMSGLTVLASAAPVFAAEDMSKVETGDQGYTVVQSK
                     YKKAVEQLQKGLLDGSITEIKIFFEGTLASTIKVGAELSAEDASKLLFTQVDNKLDNL
                     GDGDYVDFLISSPAEGDKVTTSKLVALKNLTGGTSAIKVATSSIIGEVENAGTTGAKN
                     TAPSSAAVMSMSDVFDTAFTDSTETAVKLTIKDAMKTKKFGLVDGTTYSTGLQFADGK
                     TEKIVKLGDSDTINLAKELIITPASANDQAATIEFAKPTTQSGSPVITKLRILNAKEE
                     TIDIDASSSKTAQDLAKKYVFNKTDLNTLYRVLNGDEADTNRLVEEVSGKYQVVLYPE
                     GKRVTTKSAAKASIADENSPVKLTLKSDKKKDLKDYVDDLRTYNNGYSNAIEVAGEDR
                     IETAIALSQKYYNSDDENAIFRDSVDNVVLVGGNAIVDGLVASPLASEKKAPLLLTSK
                     DKLDSSVKAEIKRVMNIKSTTGINTSKKVYLAGGVNSISKEVENELKDMGLKVTRLAG
                     DDRYETSLKIADEVGLDNDKAFVVGGTGLADAMSIAPVASQLRNANGKMDLADGDATP
                     IVVVDGKAKTINDDVKDFLDDSQVDIIGGENSVSKDVENAIDDATGKSPDRYSGDDRQ
                     ATNAKVIKESSYYQDNLNNDKKVVNFFVAKDGSTKEDQLVDALAAAPVAANFGVTLNS
                     DGKPVDKDGKVLTGSDNDKNKLVSPAPIVLATDSLSSDQSVSISKVLDKDNGENLVQV
                     GKGIATSVINKLKDLLSM"
     gene            complement(2588..4177)
                     /locus_tag="KPH29_003444"
     CDS             complement(2588..4177)
                     /locus_tag="KPH29_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp12"
                     /protein_id="HBG1469007.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETAAK
                     IAQKQTYENVVLVNTDNTLADGLSASGLAGTVKAPILLSQRNSIPSDTEKMLKDVKKV
                     YIIGTEDSIGKSVENELKQKGIEVKRIGGNDRIETSYLIAKEIASIKPIDKVFITNGY
                     TGEADAMSASSVASRDGSPIILTNGKNVPFEKKEGVQCYALGSEEIISNDLVKKTNAV
                     RLAGEDRFETNKKVIKHFYSSAKEFYLSKGYQLVDAVAGSSIAKNAPIVLVDGNSDKS
                     VLRSADKITALGGIDEKTLEQCLSASSLDASAPTITVGNLNIYQGDKFDISKLNIVAK
                     DSNGNDLTPELIGNIDTDKVGKYKVTIKATDIGGKTTSISVEVNVLEYKTNDMNSSEF
                     KRMVSSEMYSLVNSYRKEKGKEPLQVSKNLEGLSNSWSKYMADKGQFSHVINGKKSVE
                     VFTGYGLRSEENIAFVPLVTKSTYTTKDAREVANVIFTVWKKSDKYNENMLNSDFVYT
                     GFGLYILPNGEVYATQEFLNK"
     gene            complement(4396..5997)
                     /locus_tag="KPH29_003445"
     CDS             complement(4396..5997)
                     /locus_tag="KPH29_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HBG1469008.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADRQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNPLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSDKTV
                     LEGAKNITSVGEINEKVIQQCINASKSNGQPPTITVGSTEIYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSTGKRVEIKVLDDKSYDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLVVSSRLEGMANAWSKYMMDKKVFAHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKNVQTTQDAKNLAKAIFEVWKKSPEYNANMLSDEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGSL"
BASE COUNT         1752 a         1179 c          660 g         2495 t
ORIGIN      
        1 aaatttagtc tctttttttg tattcgaaaa cataatataa aaagacttct catataagaa
       61 gcctttttat attattatat ttatattaca tacttaataa atcttttaat ttatttataa
      121 ctgaagtagc tataccttta ccaacttgaa ctaagttttc tccattatct ttatcaagaa
      181 ctttacttat agatacactt tgatctgaag ataaagaatc agtagctaat actataggtg
      241 ctggagatac taatttattt ttatcattat cagaaccagt taatacttta ccatctttat
      301 ctactggctt accatcagaa ttaagagtta caccaaagtt tgctgcaact ggagctgctg
      361 ctaaagcatc aactaattga tcttctttag tagaaccatc tttagctaca aagaaattaa
      421 ctactttttt atcattattt aagttatctt gataataaga agattctttt ataacttttg
      481 cattagttgc ttgtctatca tctccactat atctatctgg agatttacct gtagcatcat
      541 ctattgcatt ttcaacatct ttagatacac tgttttctcc acctattata tcaacttgtg
      601 aatcatctaa gaaatctttt acatcatcat ttatagtttt agcttttcca tctacaacta
      661 ctattggtgt agcatcacca tcagctaaat ccattttacc attagcattt cttaattgag
      721 atgcaactgg agctatactc atggcatctg ctaatcctgt tcctccaact acaaatgctt
      781 tatcattatc aagacctact tcatcagcta tttttagaga agtttcatat ctatcatctc
      841 ctgctaatct tgtaacttta agtcccatat cttttaattc attttctact tctttagata
      901 tagaattaac tccaccagct aaataaactt tctttgaagt atttatacct gttgtactct
      961 ttatattcat aactctcttt atttcagctt ttacgcttga atctaattta tcttttgaag
     1021 ttaataataa aggagctttc ttttcagaag ctaaaggaga agctacaagt ccatcaacta
     1081 ttgcatttcc tccaaccaat actacattat caactgaatc tctaaatata gcattttcat
     1141 catcagagtt ataatatttt tgacttaatg ctattgcagt ttctattcta tcttctcctg
     1201 ctacttctat agcatttgaa tatccattat tatatgttct taaatcatcc acataatctt
     1261 ttaagtcttt cttcttatct gacttaagag ttaatttaac tggtgaattt tcatcagcaa
     1321 ttgaagcctt tgcagcactc ttagttgtaa ctctttttcc ttctggataa agaaccactt
     1381 gatattttcc actaacttct tctactaatc tattagtatc tgcttcatcc ccatttaata
     1441 ctctgtaaag agtatttaaa tctgttttat taaatacata ttttttagct aaatcttgtg
     1501 ctgttttact agaactagca tcaatatcta ttgtctcttc ttttgcattc aatattctaa
     1561 gtttagttat tactgggctt ccagattgtg ttgttggttt agcaaactca atagtcgcag
     1621 cttgatcatt tgcacttgca ggtgttatta ttaattcttt ggctaaattt atagtatcac
     1681 tatctcctaa tttaacaatt ttttctgttt ttccatctgc aaattgaaga cctgttgaat
     1741 aagtagttcc atcaactaaa ccaaactttt tagttttcat agcatctttt atagtaagtt
     1801 tcacagcagt ttcagttgaa tctgtaaaag ctgtatcaaa tacatctgac atagacataa
     1861 ctgcagcact acttggagct gtattttttg ctcccgtagt accagcattt tcgacttcac
     1921 caataatact acttgtagct acttttattg cactagttcc acctgttaaa ttttttaatg
     1981 caacaagttt acttgtagtt actttatctc cctctgctgg agagcttatt aagaaatcta
     2041 cataatcccc atcacctaaa ttgtctaatt tattatctac ttgtgtaaac aataatttac
     2101 ttgcatcttc tgcactaagc tcagctccta cttttatagt agatgctaaa gttccctcaa
     2161 agaaaatttt aatctctgtt atacttccat ctaataaccc tttttgtaat tgttcaactg
     2221 ctttcttata cttgctctgt actactgtat atccttgatc accagtctca actttcgaca
     2281 tatcttctgc tgcaaacaca ggtgctgcag aagctaatac tgttaatcct gacatagcta
     2341 ttgctatatt cttcttattc atttcttaaa ttcctcccaa cattatatta aaattacatc
     2401 tcttcattat gtacatagaa taatcatcca tatattccta atatttctca catctctata
     2461 ataatccatt tttattcttt ttgcaagtca tttttttgaa attattattt ttcccaagca
     2521 tatgagtttt tataccctta aactccaata tttaataact taaaaaattt gattttactt
     2581 atttaaatta cttatttaaa aattcttgtg tagcatacac ttctccatta ggcaatatat
     2641 ataatccaaa cccagtatat acaaaatcac tatttaacat attttcatta tatttatctg
     2701 actttttcca aactgtaaat attacatttg ctacttctct agcatccttt gttgtataag
     2761 tactttttgt aacaagtggt acaaaagcta tattttcttc acttctcaag ccatatccag
     2821 taaatacttc aacagatttc ttaccattta ttacatgtga aaactgacct ttatctgcca
     2881 tgtacttaga ccaggaatta gaaagtcctt ctaagttttt agaaacttgt aatggctctt
     2941 tacctttttc ttttctatat gaatttacca aactatacat ttcactagaa accattcttt
     3001 taaactcact actattcata tcattagtct tatattcaag tacatttact tctacactta
     3061 ttgatgtagt ctttccacca atatcagtag cttttatagt aactttgtat ttacccacct
     3121 tatcagtatc aatatttcct ataagctctg gtgtcaaatc gttaccattg ctatcctttg
     3181 ctacgatatt cagtttactt atatcaaact tatcaccttg ataaatattt aaatttccaa
     3241 cagttattgt tggtgcactt gcatccagtg atgatgcact taaacattgt tctagtgtct
     3301 tttcatctat tcctcctagt gctgtaatct tatcagcact tctaagtaca gacttatcac
     3361 tatttccgtc aactaataca ataggtgcat tttttgctat agaagaacca gcaactgcat
     3421 ctactaattg ataaccttta gaaagataaa attcttttgc cgaactataa aaatgtttta
     3481 taactttttt attagtctca aatctatctt ctccagctaa tctaactgca ttagtttttt
     3541 taactaaatc attacttatt atctcctctg aaccaagagc ataacattgc acaccttctt
     3601 ttttctcaaa tggaacattt ttaccattag ttaaaattat aggtgatcca tctcttgatg
     3661 ctactgatga cgcactcata gcatctgctt cacctgtata accattagtt ataaaaacct
     3721 tatctatagg ttttattgaa gctatttctt ttgctataag ataacttgtt tcaattctat
     3781 cattaccacc tattctttta acttctattc ctttttgctt taattcattt tcaactgatt
     3841 ttcctattga atcttcagtt cctattatat aaaccttctt tacatctttt aacatttttt
     3901 ctgtgtctga tggtatactg tttctttgtg ataataatat aggtgcttta actgttcctg
     3961 ctaatccact tgcacttaat ccatcagcta gtgtattatc tgtatttact aatactacat
     4021 tttcatatgt ttgtttctgt gcaattttgg cagctgtctc atatctatct ttcccttgta
     4081 tcttttctat gctcgaaagt gcattgatat ttggtgctcc tgccattatt aatgatattg
     4141 tcaatcctat tgataatact ctcttattta ccttcatcga actcctccta ctaatttttt
     4201 tatataattc agctaatcat aaattacatc ctcagtagtg attccctcaa aatctataaa
     4261 ctactaaaac aaaaaacacc taaaatacca tagcctgtaa agcaataaca aaacagatta
     4321 caaaaagtac tttagatgtg attttcattt tataatatca gtaaattaac aaaaaaacaa
     4381 gatttatgga attttttaca aactaccttc attgccattt aaaaactcct gagtagcatg
     4441 cacttgacca tcagataata tataaagccc aaatccagta ctatagaact catcactaag
     4501 catgtttgca ttgtactctg gagacttctt ccacacttca aatatagcct ttgccaagtt
     4561 ttttgcatct tgtgtcgttt gaacgttctt agaatctata taaatatatg ctatattttc
     4621 ctcacttctc attccaaact cagaaaatac ttgtggagca ttttttccat ctatataatg
     4681 tgcaaatact tttttatcca tcatatactt agaccatgca tttgccatac cttctagcct
     4741 actagatact accaaaggct ctttgccttt ttcttttcta taggaattga ttaagttata
     4801 catttcagta gataccattt ttttaaattc tgggctgttg taatcatagg atttatcatc
     4861 caacactttt atttctactc tttttcctgt actttttccc cattcgtctg tagcttttaa
     4921 tgtcaatatg tatgtaccta ctctatttgt atctataaac ccatcaactt ctattggtaa
     4981 taccttccca gtgttatctt tagccactat gtttaacttg ccagtgtcaa acttttcacc
     5041 cttatatatc tctgtacttc caactgtaat tgttggtggt tgtccatttg actttgaagc
     5101 atttatacac tgttgtatta ccttctcatt tatttcacct acagaagtta tattcttagc
     5161 tccttctaat acagttttat cgcttccatc atttactagt accattggag agtttttagt
     5221 aagtggtgca gctgctattg catctactaa ttggtatcca tctgatacat agaatccatc
     5281 tgcactatta aaaaagtagt ctattacatt tttgttagtt tcaaatcggt cagttccttc
     5341 tattcttact gagtttgtat ttttaactag agggttactc attatctcct ctgaacctat
     5401 acaatatgat tgaatatttt ttacatcaaa aggcacactc tttccatctg taagtatgat
     5461 tggagctcca tctctagtag ctactgatga tacactcatt gcgtctgctt ctccactata
     5521 tgcgttagtt aatagtactt tatccacttt ttttatagtt gctatttctt tagctattaa
     5581 atagcttgtt tttagtctat cttctccacc aattcttttg acttctatat ttttagaatc
     5641 tagttctttt tctactgatt tacttatagt atcttctgta cctattatat atgctttttt
     5701 gatatttttt agacatctat ttgtgtctgc tggtatctta ttttgttgtg taaatagtat
     5761 tggagctttt gtagctcccg ataatccact tgcacttaat ccatctgcaa gtgatttttc
     5821 tgtatttact aaaattactg tttcataagt ctgtctatca gctatctttg ctgctgtttc
     5881 atatctatct ttaccgtata ttttttctac tactgatgat gcatttacac ttttgaagtt
     5941 tactaagatt aatgatactg ctagacctaa tgataatatt tttttgttca ttttcatatc
     6001 cccctgatga tttttatatt ttatatttat attgtgatta ttttctattt attttctctt
     6061 tattttccat atattataga gtatat
//
LOCUS       DAELXX010000167         6048 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.169, whole genome shotgun sequence.
ACCESSION   DAELXX010000167 DAELXX010000000
VERSION     DAELXX010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6048
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.169"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            116..1315
                     /locus_tag="KPH29_003446"
     CDS             116..1315
                     /locus_tag="KPH29_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1469009.1"
                     /translation="MKLKSKISILIIFLTLAIYFSIGLLNNEDNLKIKTIKGDSNELD
                     HININFGYALNGYINSYYTINGSNKTKKTELIKLQKDSKNTNNLIDVSSGSDLIAHVG
                     FEMNEESKDINFVIDEKKEDLEKGVFPSEGSDKEIEYEKLDMNNFTRTRYKVDKSTDD
                     YYANILASKRYKKYLYVSVLYATSDSSENNKEYSSTIDIIKINPENKKIKTIKSINLS
                     NYFSEKYQDIENIGVSYASKYEDKMYILVNVLNTNIGDNSNSKFHLLEYNLEENKESI
                     MELKTDSKMALAKAKLEANKLYMVMSNDYDNSSITELNLLTYNILDKSLDEDKYVLDN
                     REKRGIRSIEIDSNKIVAYTSRLNSILSWDNKFMLYIINKNSKEIVYEGELQFNSSNS
                     MLEFEIK"
     gene            1324..2136
                     /locus_tag="KPH29_003447"
     CDS             1324..2136
                     /locus_tag="KPH29_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1469010.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVNVTGFSSFRNIMGSTESWFIFGIIACICYAFFIWYRDF
                     NGRSKSIYTLIMLPKNRINIYISKLLNILFLVYSYTVVLTISLFIASKLLPRHMLGNV
                     TNYGFVQETIYELKMLLPYSFEILFVEYIFLLIGFVSVVFTSILINKSIYKVSTLISF
                     LFLVLEILVFIISIEFIIPYEYSDVFTVLYSSINIFVCSLISNKLLKQKIDF"
     gene            2147..3415
                     /locus_tag="KPH29_003448"
     CDS             2147..3415
                     /locus_tag="KPH29_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1469011.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPNISKFSYEEVIISKNGVRNNKNPKSRIRNDYLNIDKKYSDFVNDKG
                     KELIYKDDLNIGEAYTSVKSDLVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFK
                     ENSWKTLFNKIYKGNLYMVISETNISGSKVNNICILKIDIKNKSYKLLNRFNLGLQNG
                     YQQNINEIKFSIGSKLYLEIVNNKSKNGDIEGHDSYSNLIIYDIEDNTFNISNKFMDN
                     LNKEKNANPKEIGLLSKTKLDYMIKNNKLNLIVTDMSNSIINLVYDINSNEIKLEDYE
                     ILDLKVDYNEASSGVRKVKLLDDKIYSISGMKNSISGELKGFKGIVDVVGTKPVKFQV
                     FDMKLKKNIYEAELINGDISLQEKLFFVAN"
     gene            3781..4962
                     /locus_tag="KPH29_003449"
     CDS             3781..4962
                     /locus_tag="KPH29_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG1469012.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQVDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
     gene            complement(5104..6021)
                     /locus_tag="KPH29_003450"
     CDS             complement(5104..6021)
                     /locus_tag="KPH29_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1469013.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILAAIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
BASE COUNT         2457 a          583 c          949 g         2059 t
ORIGIN      
        1 ttcaatttaa caatttagta gcagggttat taagtttttt aattataatg tttattctaa
       61 atggaatatc ttatttgcta attaaaaaga aagtatctct ttaaggaggg gtggtatgaa
      121 attaaaatct aaaatatcta tacttattat atttttgact ttagcaatat attttagtat
      181 aggtttatta aataatgaag ataatttaaa gataaaaact ataaaaggtg actctaatga
      241 attagaccat ataaacataa attttggata tgctttgaat ggatatataa attcatatta
      301 tacaataaat ggaagtaaca aaactaaaaa gactgaatta ataaaactac aaaaggatag
      361 taagaacaca aataatttaa ttgatgtatc tagtggttca gatttaattg ctcatgttgg
      421 atttgaaatg aatgaggagt caaaagatat aaactttgtg atagatgaga aaaaagaaga
      481 tttagaaaaa ggtgtatttc catcagaagg tagtgataaa gaaattgaat atgaaaaatt
      541 agatatgaat aattttacaa ggacaagata taaagtagat aaaagtacag atgattatta
      601 tgcaaatata ctagcatcaa aaagatataa aaaatactta tatgtatctg tactttatgc
      661 tacaagtgat tcatcagaaa ataacaaaga atattcaagt actatagata taataaaaat
      721 taatccagaa aataaaaaaa tcaagactat aaaaagtata aatttaagta attatttttc
      781 cgagaaatac caagacatag agaacatagg tgtaagttat gctagcaaat atgaggataa
      841 gatgtatatt ttagtaaatg tactaaatac aaacataggt gataatagta attctaaatt
      901 tcatttactt gagtataatc ttgaagaaaa taaggaaagc ataatggaat taaaaactga
      961 tagcaaaatg gctttagcaa aagcaaagtt agaagctaat aaattatata tggtaatgtc
     1021 gaatgattat gataatagtt caattacaga gttaaattta cttacctata atatattaga
     1081 taaaagctta gatgaagata aatatgtttt agataataga gaaaaaagag gaattagaag
     1141 tattgaaatt gattcaaata aaatcgttgc atatacatca aggctaaact ctatattatc
     1201 ttgggataat aaatttatgc tatatataat aaataaaaat tctaaggaaa ttgtatatga
     1261 aggagaatta caatttaaca gttcaaatag tatgttagaa tttgaaataa agtaggtgag
     1321 ttgatgaaag atttaataag attattcgaa tttgaattaa aaagaaatat aaagaattat
     1381 atatttataa taatttgttg ttgttctttt gtaattttaa acatagtaaa aaatttaaat
     1441 gactataatt acataataga aaatgcagtt aaaagtgaac agttaacaaa aatcggaaat
     1501 acgatagaaa cggtaaatgt tacaggtttc tcaagcttta gaaatataat gggttctaca
     1561 gaatcgtggt ttatatttgg tataattgca tgtatttgct atgctttttt tatttggtat
     1621 agagacttta atggtaggag taaatctata tatacactta taatgctacc taaaaataga
     1681 atcaatattt atatatctaa attattaaat atattatttc tagtgtatag ctatacagta
     1741 gtacttacaa tatctttgtt tattgcaagt aagttgttgc ctagacatat gcttggtaat
     1801 gtcacaaact atggttttgt acaagaaaca atatatgagt taaaaatgtt acttccatat
     1861 agctttgaaa tactatttgt agagtatata tttttattga taggatttgt atctgtagta
     1921 tttacatcaa ttcttataaa taaatctatt tataaagtaa gcactttaat aagttttcta
     1981 tttttagttt tggaaatttt agtgtttata atttctatag aatttattat accttatgaa
     2041 tacagtgatg tatttactgt tctatattca agtataaaca tatttgtatg tagtcttata
     2101 tctaataaac ttttgaagca aaagattgac ttttaggagg gatattatga aagcaaaaaa
     2161 tataggaaaa tatacaatat gttttataat agtatgtttt ttaatgatta ctatgacctt
     2221 aatattgagt tattttaata aggataaact atctgtaaaa actttaaaag gcaatgtaaa
     2281 agatataggg agagtaagtg tagtatatac acctaatata agcaaatttt cttatgagga
     2341 ggtaattata agtaaaaatg gagtaagaaa taataaaaac cccaaatcaa gaattagaaa
     2401 tgattattta aatattgaca aaaaatacag tgattttgtt aatgataaag gaaaagaact
     2461 tatatataaa gatgatttga atataggaga agcgtatacc tctgttaagt ctgatttagt
     2521 acaaggaatg aatgctttaa atgattgtat aaaggtttca aataaaaatt taaaaagtgg
     2581 ggtagaagaa aattttgata ttaatttaaa taagtctgat tttaaagaaa acagttggaa
     2641 aaccttgttt aataaaatat ataaaggcaa tttatatatg gtaattagtg aaactaatat
     2701 ttcaggttct aaggtcaata atatttgtat attaaagata gatataaaaa ataagtctta
     2761 taaattatta aatagattta atttaggatt acagaatgga tatcaacaaa atatcaatga
     2821 aattaaattt tctattggaa gtaaattata cttagaaata gtaaataata aaagtaaaaa
     2881 tggagatatt gaaggacatg atagttactc taaccttatt atatatgata tagaagataa
     2941 cacttttaat ataagtaata aatttatgga taatctaaac aaagaaaaga atgcaaatcc
     3001 aaaggagatt ggcttattaa gtaaaactaa gctagattat atgataaaaa ataataaatt
     3061 gaatcttata gttacggata tgagtaattc cataatcaat cttgtttatg atattaattc
     3121 taatgagatt aaattagaag attatgagat acttgattta aaagtagatt ataatgaagc
     3181 atcatctgga gttaggaaag ttaagttact ggatgataag atttattcta tttcaggtat
     3241 gaagaacagt atatcaggag aattaaaagg atttaaaggg attgttgatg tagtcggaac
     3301 taagccagta aaatttcaag tatttgatat gaaacttaaa aagaatattt atgaagctga
     3361 gctaataaat ggagatataa gtttacaaga aaagttattt tttgttgcaa attaagtatt
     3421 agaattaatt ttagaacttt aaacttttat atgtatcaca gataagtata agtaagggag
     3481 tgtctcaaaa taaacttttt agttcatgag acactttttt tatatgaaat caaatatatt
     3541 ttcattgttt agatttattt tgataatgaa aaaaagatta tctttatttt aagaaagaca
     3601 tttatttata attatataca aaaatatttt ttaatgtttt tgtgtataat tataacaatt
     3661 taatttatat taatgcactt aaatttgaaa tagaatgctt atagtggtaa aattaattat
     3721 tagaatatta gaaataataa ataaataata cattgtactt aaaaggggga gaaatttaag
     3781 atgaacaaaa aatatattaa agcagggaat ttaattgatg gaacaggaaa tagtattatg
     3841 caaaataagg gtataattat tgatggagat actatagttg aaattgaaga tataagagat
     3901 gatttaaatg gatatgatgt gtacgattac ttagataaaa ctgttatgcc aggcattatg
     3961 aattgtcatg ttcacttgac aatggagcca gttggaaatc caaagatata ttatgataat
     4021 gtatcagatg tagagcttgt agtaaatacg ataaaacaag ttgatgctta tttggattca
     4081 ggagtaacat atattagaag cttaggttgt cccaaatatg tggatgtaca acttaaaaat
     4141 cttatagaca aagggtctat agatggtcct ggtatagtta cttcaggtcc agttatatgt
     4201 atgacaggag ggcatggata ttattttggt attgaaagtg atggagtgga tgaatgtaga
     4261 aaatcagcca gaacagtttt aaaaaatgga gtagattgtg taaagataat ggcaactgga
     4321 ggtgtaacaa ctgatggagt tgagccaggg tcaccacaat taactttgga ggaaatgaag
     4381 gctgctgtag atgaagctat aaaagcaggt aaaataactg ccacacatgc acaaggaaga
     4441 actggtataa aaaatgcagt tcttgcagga attacttcta tagaacatgg tgtttattta
     4501 gatgatgaaa taattgattt gatgcttgaa agaggaactt acttagttcc aactgtggct
     4561 gcaccatatt ttattctaaa tgcaggaaaa gaaagtggaa taactgaagc tactcttaga
     4621 aagtgtgaga ttgttgccat tccacataaa gaatcattcc taaaagcata taaaaaagga
     4681 attaaaattg cagttggttc tgatgcagga acttcttata atgaacatgg aaaaacatat
     4741 tatgaaatga agctaatgtc agattatggt atggataata tggatataat agttgctgct
     4801 acaaaaacag catctgaact tttaagaatt gataaaaatt atggaactct agaaaaagga
     4861 aagaaagcag atgttatagt tgtaaatgga aatccagtag agaatataga cgtacttgca
     4921 gatgtgttag cagtatttaa acttggtaaa aaagttagat aaatagtata aaaataaaaa
     4981 agagtgtcta aagaaatttt taaataatta tagacactct tttttattag taataattaa
     5041 atcttttata ctttagaaaa tagattctat tttatattta gatataactc tttaaaatca
     5101 ttcttatgca atataatcta tagaatctac acatacaaag tatattcttg aatcattaga
     5161 atttccaagt actataacat catttttctt acctagaact tcaacaccag ttagaaccaa
     5221 actaccagct actacagtaa cttgattatt tatatttgac atataaagat tatctaggat
     5281 acctttatta caacagcaat catcacaatc gcagttacaa ttacattctt tgtaatgttt
     5341 tttatcaaga tagtaaccta aagatgctat gaaatcttca taatctgcag gtgtaatatc
     5401 aaatgcaaca gctttaagat tacacaatga tgctaaatct acgtttgcta atggagtaga
     5461 tgcagcaagt ataaaatcaa ttatagcagc aagtattgca tctaatatag ccccaaggtc
     5521 tatcgtagga ggaataactg catcaataag tgcaattaat tcagctactc ctggtatagt
     5581 tcctatagta ttaattaatc cctcaagagt taaagggact ggaataggat aagatactct
     5641 accagctata tctattaaat cacagttaca agcagaaaat ctttcaaaag taccatcaag
     5701 tccagataaa ttatcttttg gaggagctga aagatctata cctaccaaat tagcacctac
     5761 tataaagaaa tctgagataa aagcaaattg attaaagttt acaaaaggtc ttagagcatc
     5821 atatcttaga agttctaagg cctctctcat acttttttta caacaacatt gttttatttt
     5881 aggttcttta tatttatttg gataattata gtatctatca gctacttgat aattttcata
     5941 gtatctttca tttgtatttg gataatcttc ctcatattct tgtgtaaatc taaagtcctc
     6001 tctacattta ttattttcca ctaaatacac tccttttata ttttaatt
//
LOCUS       DAELXX010000168         6038 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.170, whole genome shotgun sequence.
ACCESSION   DAELXX010000168 DAELXX010000000
VERSION     DAELXX010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6038
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.170"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..120
                     /locus_tag="KPH29_003451"
     CDS             <1..120
                     /locus_tag="KPH29_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG1469014.1"
                     /translation="DLSIREWICPSCNETHDRDINASINILKEGLRLITTQNK"
     gene            239..757
                     /locus_tag="KPH29_003452"
                     /pseudo
     CDS             239..757
                     /locus_tag="KPH29_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
     gene            784..2145
                     /gene="rlmD"
                     /locus_tag="KPH29_003453"
     CDS             784..2145
                     /gene="rlmD"
                     /locus_tag="KPH29_003453"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBG1469015.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTS
                     SQEDFDMNEFKDMLLGIDLKAELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHCETVVKL
                     IRE"
     gene            complement(2227..2442)
                     /locus_tag="KPH29_003454"
     CDS             complement(2227..2442)
                     /locus_tag="KPH29_003454"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG1469016.1"
                     /translation="MIFDILIITTLEEYTLYFKNPDTMLLENGTNIKDIQHILGHAKT
                     STTMDTYLHVTNNMRNQMVNIFEIIVE"
     gene            2469..2624
                     /locus_tag="KPH29_003455"
     CDS             2469..2624
                     /locus_tag="KPH29_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1469017.1"
                     /translation="MFSKNEILKKLIEEYDVKTTTDIQDMLKDLFTNTIHQIFPLSNF
                     NGLNIIY"
     gene            2668..3702
                     /locus_tag="KPH29_003456"
     CDS             2668..3702
                     /locus_tag="KPH29_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HBG1469018.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTTTSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKVAKETGYNLGLTGAKSNNTYE
                     KEVLKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSEFIDAYNN
                     AYDEIQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPYEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKNKKRKKI"
     gene            3789..3992
                     /locus_tag="KPH29_003457"
     CDS             3789..3992
                     /locus_tag="KPH29_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HBG1469019.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            4163..5323
                     /locus_tag="KPH29_003458"
     CDS             4163..5323
                     /locus_tag="KPH29_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HBG1469020.1"
                     /translation="MKLSKTLLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGRVSTVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFATDGLETYAIGGKVSMSDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVIIKQCLNITNNVGDSNTGIVKDTNSNNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKIDPCNVCVK"
     gene            complement(5854..>6038)
                     /locus_tag="KPH29_003459"
     CDS             complement(5854..>6038)
                     /locus_tag="KPH29_003459"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBG1469021.1"
                     /translation="EGFWGILKVEMYYLRKFDTYEQLVKAIDDYIYYYNNFRYQKRLN
                     SMSPLEFRKYLSTEIA"
BASE COUNT         2385 a          614 c         1143 g         1896 t
ORIGIN      
        1 gacttaagta taagagaatg gatttgtcct agttgtaatg aaactcacga tagagatata
       61 aatgcaagta taaatatatt aaaagaagga ttaagactaa taacaactca aaataaataa
      121 cttatgtaag aaccgtagga actacgggga tagcctgtgg agaattagta agacatattt
      181 tagtatgcaa aattctgtga agcaggaacc ctgtgacttt agtcatggga ggttcaggat
      241 gaataaacct gatggatata tatcagcaac tacagataaa tatgacccaa cagtattaga
      301 tttaattgat ttatcatact tagcttttga accatttcct gttggaaggt tagataaaga
      361 cacagaggga ttattagttt taactaatga tggaaagctt tctcatagag ttttatctcc
      421 taagaagcat gtgcctaaga cttattatgc aaaaattgat ggagtagtta ctgaagaaga
      481 tgtagaagct tttttagaag gtgtagtgct tgatgacgga tataaaacta tgccatcaca
      541 gttaaatata ttaaaaagtg atgatgaatc agaaatagag cttacaatac atgaaggtaa
      601 gtttcaccaa gtaaaaagaa tgtttgaaag cgtaggcaaa aaagtagtat acttaaaaag
      661 attgtctatg ggcaatttaa aattggatga aagtttagag ttaggtgagt atagagagtt
      721 aacagatgaa gaagtaaaat tgattgaaga aagataataa agatactata gtaaattgga
      781 ggaatgtctg tgaagaaaaa agatgttata gaatttgaag tagataaaat ggaatttggt
      841 ggtacatctt taagtcaagt tggagataga gttatccata tgaagggtgg aataagtggt
      901 caaaaggtga gagctggagt taaaaaagtt agaagtaaga aagctgaagt taagatgata
      961 gaattattag agaactcacc acttgaaact gaagaaactt gcaagcactt tagagagtgt
     1021 ggaggatgta cactattgtc tgtgccatat gaaaaacaat tagaaataaa agaaaaacaa
     1081 gttatggatt tatttttaaa gcaagattta tttggatttc agtttttagg gatagaagga
     1141 agtccagaaa acaaatatta tagaaataag atggaatata cttttggaga tgaagtaaaa
     1201 aatggacctt tgacattagg tcttcataaa aaaggaaagc atatagatat acaaactgtt
     1261 gaggagtgta tgttggttga tgaagacttc tcaaagatat tagtttctag tgtagagttt
     1321 ttcaatgaaa aaaagcttcc ttattataga acaatgaatc acaaaggata tttgagacat
     1381 cttgtagtta gaaaagggat tcatactaat gagattatgg ttaatatagt aacttcttct
     1441 caagaagatt ttgatatgaa tgagtttaaa gatatgcttt tagggattga tttaaaggct
     1501 gagttagtag gtgtacttca tactattaat gatggtttag cagatgcagt ccaatgtgat
     1561 gagttaagag tattgtatgg aagagattat atacaagaag aaattttagg tcttaagttt
     1621 aagatttcac cattttcgtt tttccaaacc aatactaagg gagctgaggt tctttatagt
     1681 attgctaggg attttattgg agattacaat gataaggttg tatttgactt gtatagtggt
     1741 actgggtcta ttggacaggt tatggctggt gctgctaaga aagtgtatgg tattgagata
     1801 gttgaggaag ctgttgttgc tgctaatgag aatgctaagt tgaatggact tactaattgt
     1861 gagtttattg ctggggatgt ggctaaggtt gttaaggatt taaaggacaa gcctgatttg
     1921 attattgttg accctcctag accaggcatt cataaggatg ctattaggga tatttgtgga
     1981 tttggtgcta aggagattgt ttatatttct tgtaatccta agtctttggt tgttgatttg
     2041 gttgatttta aggggtatgg ttatgagatt aagatggtta agtgtatgga tatgttccca
     2101 aatacgccac attgtgagac tgtagttaaa cttattagag aataacaggg tttcagactt
     2161 ttatattgtg tcttaaaagt agatttgcca cctatttgcc accataatta tttttgggtg
     2221 gcaaatttat tctacaatga tttcaaaaat atttaccatc tggtttctca tgttattagt
     2281 tacatgtaaa tatgtatcca tagttgttga agttttagca tgtccgagta tatgctggat
     2341 atcttttata ttagtaccat tttctaataa catagtatca gggtttttaa aataaagtgt
     2401 gtattcttct agagtagtga taattaaaat atcaaatatc actatacaaa ggaaagtgca
     2461 ccctttttat gtttagcaaa aacgaaatat taaaaaaact tattgaagaa tatgatgtca
     2521 aaacaactac agatattcaa gatatgctaa aagatttatt tacaaataca attcatcaaa
     2581 tatttccatt gtcgaatttt aacggtttaa acataatata ttgactgata taataaaatc
     2641 atagaatatt ttagggggaa atagattatg tttaagaaaa agttaatagc tgttgcaatg
     2701 agtataacta tgattagtgt agggagcttt tcgtatgctc attcaggaag aactgattca
     2761 tcaggaggtc atcgtgataa taaaaataaa tctggattag gaagttatca ctatcattgt
     2821 ggtggttatc cagcccattt acataataat ggtggttgtc cttatactgg aggaggaagc
     2881 agttctggaa caacaacttc ggttaataat gaagaaaagc aaaagaggtc tgttggagaa
     2941 aaaggatata atcaagggta tgaagatggg tataaaggaa attatagttc atcaaattat
     3001 agtggagatt atagtgatac atatgaatca aagtactcag aaggatatga aaaaggtaag
     3061 gctaaattag aagaggaaga aaaggtggct aaagagactg gatataattt agggcttaca
     3121 ggtgctaaat ctaataatac atatgaaaaa gaagttttaa aaaatgctta tgataccgga
     3181 tattcaactg gatacaatga atataagact aaaaagatag aagaatataa agctaaaggg
     3241 atagaggata gcaataaaga taaagaaaaa atgacatttg aagaaaacat agattctgaa
     3301 tttatagatg cttataataa tgcttatgac gaaatacaag aacaattaaa aaatgattac
     3361 actactcaag gatttgagag tgctatcaaa ggagaaagat ttgacacatc aactataggt
     3421 aatgttaaat atgctaattg gtttaaagaa ggatatgata atggtaaggt taagttacct
     3481 aaagcaaaag aaagtgtata taatcaaggt tacaatgaag aagattttag tataccatat
     3541 gaaatgaagt ctattgaaac taagttaaaa ggtgtatatg atgaaggttt agaaaaaaga
     3601 gaagaagaga aaagtagaaa tgctacttat ggggttggag ctggaacagc agctgtagtt
     3661 gctggagtag tatataaaaa taaaaaacgt aaaaagatat agatgctaga tataccaatg
     3721 atgacaagtg tataacaaat ataattttaa gtataaatat ttaaaatata gaagggaagg
     3781 ttatatctat gggatttaac tttaggaaaa gcataaatct aggaggagga ctcaaattaa
     3841 atttaggtaa gaagagtgta ggtataagtg ctggtgttaa aggtgctaga gtgtcagtta
     3901 attcaaaagg aagaaaatca gctacattga gtatacctgg aactggaata tcatatacaa
     3961 aaactagtac aaagaaacgt aaacctaaat aaatatttga tatactaaga aatagactgt
     4021 tacaaatagt ctatttttta gtatatagat ttaaaaagtg acaaaatttt gaaatataaa
     4081 ctaatttcat aaaggaaaat attcttttat gtagaattat aatatttaag taatattata
     4141 aaatttatag gaggatttca ttatgaaatt aagcaaaaca ctattggcaa tagggatgtc
     4201 tgtttcatta ttgattgcta gttgtccaac atcaaacgct cttagttcta ttaattcaat
     4261 aaaaggtaca aataagtatg aaacagcagg tttaatagca gataaacaaa attatgatat
     4321 tgcaatactt attaattctg aaaatggatt atctgatgga ttgtcagcaa gtggattgtc
     4381 aggagctaaa aatgccccaa tattgttaac taagaaagat acaatccctt cagaaacatt
     4441 aaaaagatta gaaaaggtaa aagaagtata tctaattgga ggaaaaaatt cgataggagc
     4501 atcagttcag aaaatattaa gtgataaaaa aataagcata aatagaataa gtggaactga
     4561 tagaatagat acaagtcata aagttgcaaa agaaatagga agagtaagta cggttaaaaa
     4621 agtaatatta actaatgctt ttaaaggcga accagatgct atgagtgttg cttctatagc
     4681 agttagagat aaatctccaa tagtattaac tgatggaaat actacaagtt ttgctacaga
     4741 tggattagaa acttatgcaa ttggtggaaa agtcagtatg agtgataagt taataaatga
     4801 aactaaagca acaagaatag gtggagtaga tagatacgat acaaataaac aaataataaa
     4861 taaattttat aatggctcaa aagaatttta catagcaagt gggactgatt tagtttattc
     4921 tttagttggt tctacaattg cacaaaaaac acctatagtg ttagttggaa atggaagtaa
     4981 taagagtata ttaaaaaatg caagtaaaat aactgctatt ggaaatattg atgatgttat
     5041 tataaaacaa tgtttaaata taactaataa tgttggggat tcaaatacag gtattgtaaa
     5101 agatactaat tcaaataata caaacaaacc aagtaatgag cctagctcta ataatcctgg
     5161 attaactgat aaaagtacag tatatgtaac tcctaatgga aaatcatatc atctaacaaa
     5221 aaattgttct acactaaaaa gaagtaaaac aataaataaa gtaacattaa aacaagctaa
     5281 gtcacaaggt aaaatagacc cttgtaatgt ttgtgtaaaa taatgtatta gccaaatata
     5341 tattaaaaaa gacaattaat taagattgtc ttttttattg aagaaatata aatttatatt
     5401 atgtattatt aaaaagaaaa ttattaatta taaaaaataa taataaagac gataagtatt
     5461 aaaattttat gtttccaata tttatgatat aattaatatg gcaaggaata aatatttgaa
     5521 aaagctatga gtggtgtttc catggaagtt tcttcacttt cttatgagag gaggtgaaaa
     5581 ctatggaaaa tctaatgatg agtattataa ctggtgttat agccagttac atttacgata
     5641 aattaaagag ccactctgac caatgaacta tacccactgt tttatacagt taaaacatgt
     5701 aaaagtggtt ataaagtact agtgtcaatc attagtactt tttttattat attcatataa
     5761 ttaatagcta tattttaaag tttactatct aaaaaatagc acgtcaaata aaaccctaga
     5821 tatttaaatt tttttagagc tacttaaaat aaattatgct atctctgtac ttaaatattt
     5881 cctaaattca agtggagaca tagaatttaa tctcttttga tatctaaaat tattatagta
     5941 atatatataa tcatctattg ctttgactaa ttgttcgtat gtatcaaatt tccttaaata
     6001 gtacatttcc acttttaaaa ttccccaaaa tccttcca
//
LOCUS       DAELXX010000169         5988 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.171, whole genome shotgun sequence.
ACCESSION   DAELXX010000169 DAELXX010000000
VERSION     DAELXX010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5988
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.171"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..96
                     /locus_tag="KPH29_003460"
     CDS             <1..96
                     /locus_tag="KPH29_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045009167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG1469022.1"
                     /translation="LWNPSYFVATVSENTEEQIRKYINNQKKQVK"
     gene            98..1216
                     /locus_tag="KPH29_003461"
     CDS             98..1216
                     /locus_tag="KPH29_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG1469023.1"
                     /translation="MVVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIEVY
                     KNAKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNALRDLDNAYKKFFKEKAGFPKF
                     KSKKINRFSYKTNFTNGNIMYCGQHIKLPKLGMVKIRDKQVPKGRILNATISKEPSGR
                     YYVSLCCTDVDIEAFENTNNHIGLDLGIKEFCISSCGDFIENPKYLKKSLNKLAKLQR
                     ELSRKTIGSLNRNKARLKVARLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNM
                     IRNRKLSRLISDVSWSEFIRQLEYKANWYGRQIVKVGKFFASSQICNKCGYKNEEVKN
                     LNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            1334..1995
                     /locus_tag="KPH29_003462"
                     /pseudo
     CDS             1334..1995
                     /locus_tag="KPH29_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            complement(2214..3251)
                     /locus_tag="KPH29_003463"
     CDS             complement(2214..3251)
                     /locus_tag="KPH29_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBG1469024.1"
                     /translation="MNKKLIKAKIFIWSILAISLSIVFISLLIGTIISHSFNFPFNTL
                     NPKYNYDNYIVAKNESFSDKINDINVDWISGEVRIKRSDSNKIKLIQKIPSNFSKKKL
                     AKIMVDGNSLSIYDNSTKKFFIGINLPKPTILELYLPNKLYDKITLYTTSADIYSTYI
                     RSNTVEANTISGAIGLSGNINKINLDTTSGHIDITKLNSKNATLKSTSGEIQLSGKID
                     VLNLETTNGNINLKDIYNKNLTCSSVSGKMNLSGVFSDIKAESTSSNIKINSSKMLSS
                     LYCETTSANVDLSIPDNPGFTLNFDKVSGVLDSDFELNRHSDLYTYKNGITNLYLSTT
                     NGNLTIIKNNH"
     gene            complement(3244..3930)
                     /locus_tag="KPH29_003464"
     CDS             complement(3244..3930)
                     /locus_tag="KPH29_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1469025.1"
                     /translation="MLDKIRTYIDSSFTGVNETKKVKELKDELFENLKEKYNDQIQDG
                     KTEQEAYNSVISGIGDLSELIESVKEPYSLPSELIEEKKKRALRASIAVALFIISPFL
                     FVVSVVSFGIEPTIAILLMLILIALGVGLLVYNQMTRTNYALYNDNLSNKFKEFEDFE
                     ENYNHINPVYKSFMNAYWIIVVSIYLITSILFNIWSFSWILFIIGAAGVNIVKGIIQL
                     KGYKEEDKYE"
     gene            complement(3942..4262)
                     /locus_tag="KPH29_003465"
     CDS             complement(3942..4262)
                     /locus_tag="KPH29_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1469026.1"
                     /translation="MIIASDMIRGHTETIILKHLLEKDSYGYEINKSILEKTKGLYEL
                     KEATLYTAFRRLEQSGAITSYWGDEVTGARRKYYSITNIGKEKYIKSREEWNNTKALI
                     DILI"
     gene            4623..5963
                     /locus_tag="KPH29_003466"
     CDS             4623..5963
                     /locus_tag="KPH29_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG1469027.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCM
                     VGIIVFADIIYFFDVPPNIRVIISMIITIAFYKISYDVGFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGTGDIYRIESIILGKILLIVSLFLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPGSGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDKNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDVMQNFYLIRVENTKMNKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKAVNHVI"
BASE COUNT         2282 a          657 c          911 g         2138 t
ORIGIN      
        1 ctttggaatc ctagttattt cgttgctact gtatcagaaa atacagagga gcagataaga
       61 aaatatatta ataatcagaa aaaacaagta aagtgaggtg gtagtagtgg aaaaagctta
      121 taagtttaga atgtatccaa ataaaaaaca gcaagagtta attaacaaaa cttttggatg
      181 ttgtagattt gtatataata agtatcttgc taaaagaata gaagtttata aaaatgctaa
      241 agaaactttt acttataaac agtgtagttc tgatttaacc aatttaaaaa aagaattaaa
      301 atggcttaag gaacctgaca aattttcact acaaaacgcc ttaagagatt tagataatgc
      361 atataaaaaa ttctttaaag aaaaagcagg atttcctaaa tttaaatcaa agaaaattaa
      421 tagattttca tataaaacta actttacaaa tggaaacatt atgtattgtg gtcaacatat
      481 aaaactacct aaacttggta tggttaagat aagggataaa caagtaccta aagggagaat
      541 acttaatgct actatatcta aagaaccaag tggtagatat tatgtatcat tatgctgtac
      601 tgatgtagat attgaagcat ttgaaaatac taataatcat ataggtttag atttaggaat
      661 taaagaattt tgtatctcaa gttgtggaga ctttatagaa aatcctaagt atcttaagaa
      721 gtcattaaac aaacttgcta aattacaaag agaattatcg agaaaaacaa tcggtagttt
      781 aaacagaaat aaagcaagat taaaagttgc aagacttcaa gaacatatag ccaatcaaag
      841 aaatgatttt ctacaaaaac tatctactaa attaataaaa gagaacgata ttatttgcat
      901 agaagattta caagtaaaga atatgattag aaatcgtaaa ctatctcgtt taatttcaga
      961 tgtatcatgg tcagagttta ttcgtcaatt agaatacaag gctaattggt atggtagaca
     1021 aattgtaaag gtaggtaaat tctttgcaag ttcacaaata tgcaataaat gtgggtataa
     1081 aaacgaggaa gtgaagaact taaatataag agaatggatt tgtcctagtt gtaatgaaac
     1141 tcacgataga gatataaatg caagtataaa tatattaaaa gaaggattaa gattaataac
     1201 aattcaaaat aaataactta tgtaagaacc gtaggaacta cggggatagc ctgtggagaa
     1261 ttagtaagac atatgttagt atgcaaaatt ctatgaagca ggaaccctgt gactttagtc
     1321 atgggaggtt cagattttgg ctgaagatga agaaaataat catataaata ttgagatgca
     1381 aagagctttt gagaacgatt atttggaaag agctgaatat tatcttagtc gtgtacatgg
     1441 tagaaagtta gaagaaggta aagagtataa agaaattgga aaaactattg gaatacatat
     1501 actaaatcat gtaaaatata atcacataga agattatgta aattgcctga gattaacaat
     1561 ggacgagcat ccagatattt attctagtaa aactgcatta tattttatag agcttccaaa
     1621 aattcataaa tctgactata tagtaaatag aattatgcta tggggaaaac ttattgataa
     1681 tccttctagc tcagatgtaa gagttatagc taagaatgat tctattatta aaaaagcact
     1741 tgatagatta aaagaattag gtagcaatga agaatattta ttaaatctga aaagaggtgc
     1801 ttatatcatg aataaaagta gaaattttaa agaagagatt gaaagagaga cagcaattag
     1861 agttgcaaaa gaaaaagatt ataaaataat tataatgttg aaaaaaatgg ttttaaatta
     1921 gatgacatat tgaataaatt taatgatttt gacttaagtg aagatgagat aaaagaagta
     1981 tatgaaaata attgatgata aataaaaagt gaacttatat aagtttgctg aatctgagcc
     2041 tggaaatatt tatgtgtatt ttttaatgaa taaaaattta ttttaaaaac aataattaga
     2101 ggtacagtta attatatttt tatgatactg tacttctagt tttttctatt gtagatttat
     2161 tatttactaa taaatctaca atagaagtgc ttttgttgta agaatcattt tatctaatga
     2221 ttgtttttta taatagttaa atttccattt gtagtgctta aatataaatt tgtaatacca
     2281 tttttataag tgtataaatc actatgacga ttcaactcaa agtcactgtc caaaacacca
     2341 gaaaccttat caaaatttaa agtgaaacct ggattatcag gaatagacaa atcgacattg
     2401 gcagaagtag tttcacagta cagagaagac aacattttgc tagaattgat ttttatatta
     2461 ctgctagttg actcagcttt aatgtcagaa aaaactccag ataagttcat ttttccagaa
     2521 actgaactac aagtaagatt tttattgtat atatctttta aatttatatt gccattagtt
     2581 gtttctaagt ttaaaacatc aattttccca gaaagctgta tttcaccaga tgttgatttg
     2641 agtgtagcat ttttactatt tagttttgtt atatcaatgt gaccactagt tgtgtctaaa
     2701 tttattttat taatattacc agagagacct attgcacctg aaattgtgtt agcttcaact
     2761 gtattacttc ttatgtaagt gctatatata tctgctgaag tagtatataa ggttatttta
     2821 tcataaagct tattgggcaa atatagttca agtattgttg gttttggaag attaattcca
     2881 atgaagaatt ttttagttga attatcatat atagaaagag aatttccgtc aaccattatt
     2941 tttgctagtt ttttctttga gaaattacta gggatttttt gaattaattt gattttatta
     3001 gaatcacttc tcttaattct aacttcacct gaaatccaat caacattaat gtcatttatt
     3061 ttatctgaaa aactttcatt tttagccact atatagttat cataattgta tttaggatta
     3121 agagtattaa aagggaaatt aaaagaatgt gaaattatag ttcctataag gagtgagata
     3181 aatacaatag aaagtgatat tgccaggata ctccagatga aaatctttgc ctttataagc
     3241 tttttattca tatttatcct cctccttgta gccttttagt tgtataattc ctttgacaat
     3301 gttgacacca gcagcaccta ttataaatag tatccacgaa aaggaccaaa tattaaatag
     3361 tatgcttgtg attagatata tagaaactac tataatccaa tatgcattca taaatgattt
     3421 ataaactgga tttatatgat tataattttc ctcaaaatcc tcaaattctt tgaatttatt
     3481 tgataaatta tcattgtata gagcatagtt tgtccttgtc atttgattat agactaataa
     3541 acctactcca agtgctatta atatgagcat taaaagaata gctatagttg gttctatgcc
     3601 aaaacttaca actgagacta caaataggaa agggcttatt ataaaaagag caactgctat
     3661 tgatgctctt aaggctcttt tctttttttc ttcaattaat tcagaaggta gagaatatgg
     3721 ttccttaaca ctttcaatta actcagataa atcaccaatt cctgagatta ctgagttata
     3781 tgcttcttgt tctgttttac catcttgtat ttggtcgtta tacttttctt ttagattttc
     3841 aaataattca tctttaagtt ctttgacttt ttttgtctca ttaactcctg taaagctact
     3901 gtcaatataa gtacgtattt tgtctaacat attttcacct cctaaattaa tatatctatc
     3961 aatgcttttg tattattcca ttcttcacgg ctttttatat atttctcttt acctatgttt
     4021 gttattgaat aatactttct tctagctcca gtaacttcat caccccaata agaggttatt
     4081 gcaccagatt gttcaagtct acgaaatgct gtatacaaag tagcttcttt caactcatac
     4141 aatcctttgg ttttttcaag tatagattta ttaatctcat aaccataact gtctttttca
     4201 agtaaatgtt ttaagataat tgtttctgta tgacctctta tcatgtcaga tgcgattatc
     4261 atgaaaatca cctccaatta aatatataat actttgatat acttcgcctg tcaaggtata
     4321 tcatgagaaa agacatttta ttgtaatatt tataaggttt tatatagaaa taatgtaaaa
     4381 actttataaa taatatattt tatacctagt tatagataac tttatatagt tattattaaa
     4441 aaaagcaata aaaagtttaa ttcaatttag caaatttagt gatataatta aaaaagaaaa
     4501 gaaatatgga cttatttgga ataattttga caatctattt actacacgtg tagagtcgta
     4561 tataattaaa ttatacataa aatataaaaa aataatttag taatacgtta ggagaaagta
     4621 ttatgataaa gtcaagctta ttttggaata ttttcattgc gatatccttt atgatagaat
     4681 ggtttctttg taagaaaatg ctagattata caagtgagaa aaggatttct aacaaaaaaa
     4741 taaacttttg tatggtaggt attattgttt ttgctgacat aatatatttt tttgatgtac
     4801 ctccaaatat aagagtaatt atatctatga taattactat tgcattttat aaaatctcat
     4861 atgatgtggg atttataaag tctgtattaa tatccttaat ttattggatg ttactaatcg
     4921 ccacagatgc tttgagtata tctttaacag tttggcttaa ttctataaat gacatgagta
     4981 gtatggggac gggagatata tacagaattg agtctataat attgggaaag atacttttga
     5041 ttgtgtcttt gtttttatat agagccttaa agataaaact taatatagaa ataaataaaa
     5101 ggacattatt ttatatagtt attcctatag tcgctaatat agcatctttt tttgtaattt
     5161 ttgaatacat atttaatcca gggtctggta gcttaataca taatttccaa ttcgttatga
     5221 tttctgttgt attgcttata tcaagtatca ctttagtttt aattgctaaa aaattacagc
     5281 aatataatac tttagttgta gaaaacaata tgattaaaaa tactttgaag tatgaaaatg
     5341 agtattatga agatatagaa aagaaatatt taaaaactag attactaagt catgatatga
     5401 aaaatcacat actgtgtata aatgctatga ttaaaaaagg tattgatgtt acttcttatt
     5461 taaataattt gcaggtagaa attcagagta atgacttgtt atttaatact ggtaatttta
     5521 tattagatgc tatattaagt gaaaagaaag aagtttgtga taagaatgat atagaactaa
     5581 aaataggaat aaattttagt tgttgtgaag ctgttgaatt agtagatata tgtcatatat
     5641 tttcaaatat tttagacaat gcaattgagg catgtaataa aattgatagt ggaaatagag
     5701 ttatttgtgt taaaggtgat gttatgcaaa atttttattt aataagagtt gaaaatacta
     5761 agatgaataa gattattact aaaaatggag atattttgac agacaaaaaa ggttctcttc
     5821 atggacttgg aataaaaagt gtaaaaaact ctgtaaataa gtatggtggt aatgttgtaa
     5881 ttgaatatga tgataaaaaa tttgtagtta aaattgctat tccacttaga aatgtgaaga
     5941 aagcagttaa tcatgtgatt taaatgtata taaatttgat gtgtagaa
//
LOCUS       DAELXX010000170         5964 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.172, whole genome shotgun sequence.
ACCESSION   DAELXX010000170 DAELXX010000000
VERSION     DAELXX010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5964
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.172"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            439..744
                     /locus_tag="KPH29_003467"
     CDS             439..744
                     /locus_tag="KPH29_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1469028.1"
                     /translation="MPSMIMEVVGVLFIAIGLILTFIEIKGNFPSFYGFGQNSIGKNA
                     VGIIAAGIALIFFRNGIDSIDSIATYGVFLAIELVVIVAYFLIRKKFTKEDKHLRTN"
     gene            1152..1841
                     /locus_tag="KPH29_003468"
     CDS             1152..1841
                     /locus_tag="KPH29_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1469029.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYFVKTAVDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSEDMVIQYKDLIINISKYVVSKNGIDLNLTHTEF
                     EIVKLLATNQGRAFSKEQLYNTIWKEPYYGNENVLNTHINRLRNKLSDGIEKNNYIKT
                     LWGIGYKMEEE"
     gene            1841..2758
                     /locus_tag="KPH29_003469"
     CDS             1841..2758
                     /locus_tag="KPH29_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG1469030.1"
                     /translation="MELFLVVISIVLFAFYLKERNKSKKMESDYKYINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLLEKFYSRQINYNRSEKAILQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRATLEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHVFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLANSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            2831..3757
                     /locus_tag="KPH29_003470"
     CDS             2831..3757
                     /locus_tag="KPH29_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1469031.1"
                     /translation="MNYIIETHQLKKIYKDKAVVNDVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVKPNSGEIVMFSKKVAETDFEILKKIGTIIENPYFYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     KNQIGINSIGRKQSSLEDYFFQTTGTGGIETL"
     gene            3754..4434
                     /locus_tag="KPH29_003471"
     CDS             3754..4434
                     /locus_tag="KPH29_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1469032.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSILGILLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLVFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIVFCSLGI
                     WTFVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
     gene            4879..5580
                     /locus_tag="KPH29_003472"
     CDS             4879..5580
                     /locus_tag="KPH29_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG1469033.1"
                     /translation="MNNSFLDKLIETKELKNSLYNVLKHNFLYHSNKIEGSTFTTEAL
                     ALLLDKNVVTGRHTLDDVQETVNSSYVFDTVIDSLKEKITHNFLRNLHSSLIFNTTLH
                     SRGMAGIYKTIPNMILGTDVSIAQPFEVEPKLDELIEWYYSQSEVSIKVIAEFHYRFE
                     LIHPFQDGNGRIGRFVMLKQMLENNLPIKIVSWDSEDLYRNSLNSCSLGNYVPLIEYL
                     SSLEDFREVYKMLWK"
BASE COUNT         2342 a          613 c          906 g         2103 t
ORIGIN      
        1 ataaatgact tataaataaa aagaaagctt atagattaga gaatgaatta attataggta
       61 aaaaacaagc ttatagatta gagaataaat gacttataaa taaaaaccaa gcttatagat
      121 tagagaatga attaattata aataaaaacc aagcttatag ataagaagtc catgcttata
      181 agccagaaaa tgaatacttt aatagattaa aaaatacata tttgtgaatt atagaatgaa
      241 taactccata aactagaaaa tttataataa tatctatatg aaaattagaa ttattttatc
      301 acaaaaaaat taagttaaat aaatatgaaa ttacttccaa atatacttcc aaaaacatgt
      361 aatcgcatat ataatataat tataaaaata ataaatattt ttatagtagt agttaattat
      421 taataaggag gggtcaaaat gcctagcatg attatggaag ttgtaggagt actatttata
      481 gctattggat taatcttgac attcattgaa ataaaaggaa atttcccttc tttttatggt
      541 tttggtcaaa attctatagg gaaaaatgct gtaggtatta tagcagcagg aattgcttta
      601 attttcttta gaaatggcat agatagcata gattctatag ctacttatgg tgtattttta
      661 gcaatagaac ttgttgtaat agttgcatat ttcttaataa gaaaaaaatt tactaaagaa
      721 gataaacatt taagaactaa ttaactaaat tatctactat aaaattggaa aattaaaaac
      781 aaataaaagt ttgtttttaa ttatttaaaa tcccaatttg attattttta aagaaactat
      841 attctgtata aaactagaat gtggtttctt ttttatgtat aaaatatttt ttagcaaaag
      901 ttaaaaggtg ttttataata taaaatagtt aattgtagat gttaataaaa gaaaattatg
      961 tatatttcag ataaagataa gagagtatac tatattttaa ttattttttc aataaaactt
     1021 attgaaaaga actttaactt agaagagtat caccaaattg cagatacaat atcataagca
     1081 gttatttatt gacaaccctt tttaataaat atatcatttg aagtatactg acgaaaagga
     1141 gttgaataat aatggacatc ttattggttg aagatgataa gaatattcga gaaatggtat
     1201 acgattttct gattagtgag aattattttg ttaaaactgc cgttgacgga aatacagcta
     1261 ttgaaatatt taaaaattat tcatttgata ttattttact ggacttgatg ttaccaaagt
     1321 ctagtggttt tgaagtatta aaatatatac gaagtgtgag tgttgttcct gttattatta
     1381 ctactgcgaa agatagtgac acagataaaa tgctagggct taatcttggt gcagatgatt
     1441 atattacaaa gcctttttca atcgtagaat tgcttgcaag aattaaagct aatattagac
     1501 gtgcaacggt gtatagcagt tcaagaaact ctgaagatat ggttattcag tataaagatt
     1561 tgattataaa catatcaaaa tatgtagttt caaagaatgg aattgactta aacttaacac
     1621 atacagaatt tgaaattgtt aaattactag cgactaatca aggacgtgct ttttcaaagg
     1681 aacaattata caacactatt tggaaagaac cttattatgg aaatgaaaat gtgctaaata
     1741 ctcatattaa caggttaaga aataaattgt ctgatggtat agaaaaaaat aattatatta
     1801 aaactctttg gggaattggc tacaaaatgg aggaagaata atggagttat ttttagttgt
     1861 aatttcgatt gtactatttg ctttctactt aaaagaacga aataagtcaa agaaaatgga
     1921 aagtgattat aagtatatca attcaagact tcgtgatata acaaatgatg atgaaattaa
     1981 ttatatttta gtaccatctg atatttcgat tgtaaaagaa acagcagtag atataaataa
     2041 acttcttgaa aagttttaca gtaggcaaat taattataat agaagtgaaa aagcgatatt
     2101 gcaaatattt acaaacatat cacacgattt acgaactccc atcacagtat taaagggtta
     2161 tattgaaatg ctatatcttc aaagtcaaaa agaagaatta tcttcttcaa tgagagcaac
     2221 tttggaaaaa atgcaaacaa attcaaatga gcttgttaat tcaataaata acctgtttaa
     2281 tatggcaaaa atccaatctg gagatatgat attaaatctt caaaaatcca atctaacaca
     2341 attatgtcat gagattattt tagagtttta tgatttacta gataaagaaa actttaatgt
     2401 tgaagttaat attgaagata gacctatata tgcaaatgtt gacacagaag cattacaacg
     2461 tatactcaaa aatttaattg ataatgctat aaaatatggt tcagatggta gatttttagg
     2521 gatttcacta tatgaaaagg aaaaacatgt ttttatagat attgaggata aaggttcagg
     2581 tatttctgaa actgaaaaag agcatatttt cacaagagct tatactgctg acagaaaaaa
     2641 aggcaatggt ttaggtttgg ctatttctca agggcttgca aactctatgg gaggttcaat
     2701 ttatgtaaat agtagaccta atgtaaaaac agtttttacg gtaaaattga aaagttagaa
     2761 aaaagttaga tttaaaagag agaaaagata attttatttt gtatactgtt tttagaaagg
     2821 agggagtatg atgaactata tcatagagac ccatcaatta aagaaaatat ataaagataa
     2881 agctgttgtt aatgatgtta atattcatgt gaaaaaggga gaaatttatg gatttgttgg
     2941 accaaatgga gcaggtaaaa gtacagtaat gaaaatgttg ctaaatctcg ttaagccaaa
     3001 ttctggagaa atagttatgt tcagtaagaa agtggcagaa actgattttg aaatattaaa
     3061 aaagattggt actattattg aaaatcctta tttctatgag aatttgactg ctaaacagaa
     3121 tcttgattta cactgtgaat atatgggtta ttataacaaa gaacatatat cagaggtatt
     3181 ggaatgtgtc ggattgtcaa agcaatcaaa caagaaagtt tcaaaatatt ctttagggat
     3241 gaaacaacgt cttgcaatag ctagagcaat tcttactaaa ccagaattat tgattcttga
     3301 tgaacctata aatgcactcg acccagaggg gataagagaa atgcgagaat tattccgtaa
     3361 gttaaataca gattatggaa taactatatt tatttctagt catattcttt cagaggtaga
     3421 acaaattgcg gacactattg gaattattca agatggtaaa cttattaaag aaatttctat
     3481 gtcagatata cacaaatatc aaacagatta tgttgaagta gatgtagata atgtggaact
     3541 tgctggatac ttgttagaaa aagaatttgg aataaaagac tttaaaatta cttcggaaag
     3601 ttgtattgaa atatatgatt tacgaaaaga tgtaaaagaa atatccaaaa tatttatcaa
     3661 aaatcaaatt ggtataaata gcattggacg taaacaaagt tctttagaag attacttctt
     3721 ccaaactaca ggtacaggag gtatagaaac actatgatac atttaatgaa actagaatta
     3781 aaaaaagttg ggctaaaaag atatttttta ttttcaatac tggggatttt gttatgtatg
     3841 ttttttgtct ttactggatt gaatgataaa tctacttcaa tatatgatta tgatgttaca
     3901 tttaggacaa tcagtttagt tttctgtttt tactatatta tattatttgc tgtgttgaat
     3961 gtggcatata taataaatga atatacaaat aaaacaatct tgattttatt ctcttatcca
     4021 cttgaccgta aaaaacttat tttatcaaag ttacttttga ttacgactct tatgatatca
     4081 tcaatgatag ttggatatat ctgttgtttt acattcgttg ttgtgttgga taagtatttt
     4141 aatttagtta ttggagattt tcatatttca attttatctt attggatacc aggagcaatt
     4201 aaaagtatta ttgtattttg ctcattaggt atatggacat tcgttgttgg aatgataaga
     4261 aaatcaatac ctgttaccct tgtcagttca attattttta tcaatttaag acaatttatt
     4321 ttagctggta caaatacaac tcaagacagt ctgatttctg tttttattat agttggaata
     4381 actgtgattg gtatatatta tatactaaat cataaagtta ctgaaattga ctagatactg
     4441 tggataggaa ggtagttatt aattgtatct tcttgggata ataatgattt gctattatat
     4501 cagctattat taatattatt agagttttaa taatattaat aatagctata tattttttct
     4561 tcttatttat aaatttaatg atttatagtc ataaaatgaa ctctaagaag agataaaaat
     4621 gcgttttata tgtttttaga ttaatatgaa atatcacaat gaaatgttgt acaaagtgat
     4681 atataataat tatatgaact ataaaattga ctaaacttat tgactaataa attagatgaa
     4741 aagttgagac ttactgaaaa taaatacaat gaaatatata ctacaagtat taaattaaat
     4801 ctgaacttta catactatta gaataagtat tataagatta taatgattta aaaatattaa
     4861 aataattgag gtgataacat gaacaattcc tttttagata aattgattga gacaaaagaa
     4921 cttaaaaatt cactttataa tgtgttaaaa cacaattttc tttatcattc taataagata
     4981 gagggcagta cttttactac tgaagcatta gcacttttac tcgataagaa tgttgttaca
     5041 ggaagacata ctttagatga tgtacaagaa actgttaatt ctagttatgt atttgataca
     5101 gtaattgact cactgaaaga gaaaataaca cataatttct taaggaatct acattcaagt
     5161 ttaatcttca atacaaccct acattccaga gggatggctg gtatatataa aactataccc
     5221 aatatgatac taggaactga tgtaagcata gcacaaccat ttgaagttga gccaaagctt
     5281 gatgaattaa ttgaatggta ctatagtcag agtgaagtgt ctattaaagt tatagctgaa
     5341 tttcattata gatttgaatt gatacatcca tttcaagatg gtaatggtcg cataggtaga
     5401 tttgttatgc taaaacagat gttagaaaat aatcttccta taaaaattgt atcttgggat
     5461 agtgaggact tgtatagaaa ctccttaaat tcttgtagtt taggtaatta tgtcccttta
     5521 attgaatatt tatcaagttt agaagatttt agagaagtat ataaaatgtt atggaaataa
     5581 ttgtttgtag caaaaaaagt ttttatataa atatattttt aactatgcta tttaacgttg
     5641 atttcttagt tattcttagc ttggactata cccactattt taactatata aaatagtgga
     5701 tatagtttaa ctatctcttt ataactattt ttatgtatct tagttgtttc ttaatattgg
     5761 gacagttcat aaaactaaaa gtgattttta attttatcat ttataattta ccattacatc
     5821 agaaaaatgc ttatcattag aattttcata aaaggtacct attttcttca attaactaaa
     5881 tcaattctat tagtcttaat ctttcgtatt tcaaattgat aatacaagaa aataaaaaat
     5941 aaaaaatttt aaatttttta ttta
//
LOCUS       DAELXX010000171         5802 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.173, whole genome shotgun sequence.
ACCESSION   DAELXX010000171 DAELXX010000000
VERSION     DAELXX010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5802
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.173"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(286..2118)
                     /locus_tag="KPH29_003473"
     CDS             complement(286..2118)
                     /locus_tag="KPH29_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1469034.1"
                     /translation="MSRMGRGPMGKSMEAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVK
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            complement(2111..4357)
                     /locus_tag="KPH29_003474"
     CDS             complement(2111..4357)
                     /locus_tag="KPH29_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1469035.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            complement(4425..4886)
                     /locus_tag="KPH29_003475"
     CDS             complement(4425..4886)
                     /locus_tag="KPH29_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG1469036.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            complement(5191..5757)
                     /locus_tag="KPH29_003476"
     CDS             complement(5191..5757)
                     /locus_tag="KPH29_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HBG1469037.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
BASE COUNT         1853 a         1078 c          626 g         2245 t
ORIGIN      
        1 attctattta taataaaatt taaagaataa aattaaatat acattttgat aatcacctcc
       61 aacattttcc ttactacata ttataataaa ataaacactt tgtaaagaca taaaattatg
      121 tggctatctc aaaatataga taatcctctt tttcattgtt aaaatgataa aaatacactt
      181 tatttcatac acaaaagagt gccttatgaa ttaaaaagtt cattttaagg caccctcatt
      241 tattttatct tattaattac atgtctatgc taagatagtt ttttgttaca ttgcttcatc
      301 ttcttcaaat tgagaattgt aaagattggc atagaatccg cctttcttta gaagttcctc
      361 atgatttcct tgttcaacta tatctccttc attcattaca agaatcatat cagcatcacg
      421 tatagttgat aatctgtggg ctataacaaa acttgttcta ccttccataa gattatccat
      481 agctttttga atcaatactt ctgttctagt atcgacagaa cttgtagctt catctaatat
      541 taaaatttta ggatcagcta aaatagcacg agctatagtc aataattgtt tttgaccttg
      601 agaaacatta cttgcttctt catttaattt catcttatat ccatctggta acgtcttaat
      661 aaatctatga acatgtgcag atttagcagc ctctataact tcttcatctg tagcattcaa
      721 cttaccatat cttatatttt ccataattga accactaaat aaccatgtat cttgaagaac
      781 cataccaaac atttctctaa gctcactacg attaaaatct ttaatattat gaccatcaat
      841 cagtattgaa ccgctattaa catcataaaa tctcattaac agtttaacta ttgtagtttt
      901 acctgctcct gtaggtccaa caatagcaac tttttgacca ggtttaacat ttactgagaa
      961 atcatttatt atagtcttgt ttgggttata tccaaagttg acattttcaa aatcaatttt
     1021 accttctaaa ccatctattg aaactgcatt ttcaacaatt tggacttctt cttcctcttc
     1081 taaaaactca aatactcttt cagaagctgc tgctgttgat tgcaataaat tggcaacctg
     1141 tgcaatctga gtcattggct gtgtaaagtt tcttacatat tgtataaatg actgtatgtc
     1201 accaacctca attgttttct taattgcaag ccaacctcct aagattgata ccattacata
     1261 tcccaagttt ccaataaaca tcataagtgg ttgcattata cctgataaaa attgagattt
     1321 ccacgctgaa ttatacaggc tatcatttag tttatcaaac tcttcaatta cttcatcttc
     1381 acgattaaat gctttcacta ttgtttggcc actataagtt tcctcaactt gaccatttac
     1441 atttcctaaa tattcttgtt gatatctaaa gtatttttgt gatttcttta ttattcttga
     1501 aattacaaac attgatattg gtaatattaa aactgccact aatgtcataa ttccacttat
     1561 agataacatc attatcaaaa cacctatcat agttgttact gatgttataa gctgtgtcat
     1621 actttgattt aaactttgat ttaaagtatc tatatcattc gtaattctcg ataatatttc
     1681 accatgcgtc ttagtatcaa aatacttcat tggtaatcta tccaattttg ctgaaatctc
     1741 ttttcttaaa ttataggata ctttttgaga aattccactc attataaatc cttgtataaa
     1801 agagaacagt gcacttatca aataaaggaa aagtaaaaag gttaagattt ttccaattgc
     1861 attaaaatct atacctacgt ttcctccaga aactttactc actaaccctt caaatatctt
     1921 tgtagttgcc tttcctaaaa tttttggacc tactatagaa aacgatgcac taccaatagc
     1981 aaatacaatt actaaaatta ttgatatttt aaatttagat aaatatgcaa ttagttttcg
     2041 catagtccct ttaaagtcat ttgctttttg tcctgcttcc atagattttc ccataggacc
     2101 tcttcccatt ctactcatta gctagctcct cctttgaaag ttgtgataaa gctatttgct
     2161 cataaacttc gcaatttttt agaagctctt tatgtgttcc tattccaact atttctccct
     2221 catttaaaac aattatctgt tctgcattta agatagtact aattctttgt gcaactatta
     2281 gaactgtact ttcagcagtt tttgatttta aagccttacg aagagctgca tcagttttta
     2341 agtcaagtgc tgaaaaacta tcatcaaata tataaacttc tggatttttt gctattgctc
     2401 ttgcaataga aagtctttgt ttttgacctc cagatacatt tgttccacct tgagaaattt
     2461 cagttttaaa agcttctggt ttagaatcta taaattctgt agcttgagct atttcagctg
     2521 ctgttctcat ttcttcatct gttgcgtctt ttctaccata ttttaagttg gattctatag
     2581 taccagaaaa caatactccc ttttgaggta catatccaat tttttccctt aaatcatgtt
     2641 gagaaacatt ttttatatta actccaccaa gtaatatttc tccttcagta acatcaaaga
     2701 aacgaggaat taaatttata agagttgact taccactacc agtacttcct ataaaggcag
     2761 ttgtttctcc tggctttgct gtaaaagata tatttgttaa tataggttct tctgcgtctg
     2821 gatacatgaa cgaaacattt ttaaattcaa caagaccctt tttatcttcc ttaaactctt
     2881 gtgcctctga tttttctggg tcttttatgc ttatatctga cactaatact tcatctatac
     2941 gtgtagctga aacggatgct cttggaagca taatagatac cattgatatc attaagaatg
     3001 acattattat ttgcattgta tattgtataa atgccatcat atctccaact tgcattgctc
     3061 cagaatcaac actgtatgca cctctccaaa ctataagaac tgtgataagg ttcattataa
     3121 gcatcatagc aggcatcata catgtcataa ctctatttac aaaaagattt gttttagtta
     3181 aatttttatt tgctttatca aatctttctt cttcatgttt ttcagtacta aaagctctga
     3241 taactggaat accagtaatt atttcacggc ttactaagtt taacttatca acaagctttt
     3301 gaaccagttt aaacttaggc ataaccactg agaataacac tacaattaat gataagatag
     3361 caactactgc tactgctata atccaggcca ttgaagtatt tgtgtttaaa acctttatca
     3421 ctccacctat agccattata ggtgcataga atactattct aagcaacata accatcaaca
     3481 tttgtatctg ttgaatatca tttgtactac gagtaattaa tgaagctgta gaaaattcat
     3541 tcatttcagt atttgaaaat gacataactt ttctaaatac tccacttctt aaatttctac
     3601 ctacacttgc tgctactctt gctgctaaaa atcctactgt tattgtagct accatactaa
     3661 tcattgctat accaagcatt ttagcaccag taaataatat ataattattt tgagtttttt
     3721 ctgtatcaat acctattttc tcatattctg accttacata tgaaacagag ctttgtgtta
     3781 tcatactctc tggcatactt tcaaagcctt tgctcatctc tttagtcaat gtatctaact
     3841 gttcctttgg cattgctgat aacaatttaa atatatcaac atctccactt tgagtagctt
     3901 gtggtggaag tttactcatt aactgagctt tcatagcttt tacctcttta ctatcgccct
     3961 caagccctga tactattaaa atagctttac caaaaataac atttaaatca tctatagtgt
     4021 ctttgtcttt agtatttaac tcataaagag attcatcttt tagacctggg tacttatctt
     4081 tatacttaac ataatccttt ttattcagca aagtataatt atctagtact ttatccttac
     4141 tacttttatc cataaataaa gtaattttat ttaattcact ctctctaatc acagatggaa
     4201 cagagttttc tactccacct tgttgtatac ctacatttac tatattggat gtgtattctg
     4261 gtaaagaaag gtcacatacc gcttgaatta ccaatagaca gactattatt actattgata
     4321 atgccgattt ctttaaatat ttaattaatt taaacataca tttcttccct tctgtaatat
     4381 taattagtct aataacatta aattgaaaaa cattatttta aactttaaaa atatttttca
     4441 tcttcatgct tatgacatcc tgcttttaaa ttatctcgta cttgcattag aagtcttcta
     4501 agtactattt tttcttcttc actaaatcct aaaaaaaact ctctttccaa ttccttgtta
     4561 aattcttcaa ctttattaca tatttctaaa cctttgtctg tcaaaaaaat cctagatact
     4621 ctttggtcct tttcatcttg ttttctttcc acaagtccag ctttttccat cctctttatc
     4681 ataactgtag ttgtagatgc ttttactttc cttttatctg ctaattcttt ttgactttgc
     4741 ccatctttca tctttaaaga aaaaagtatt tggggttgac catgatataa gcctaattta
     4801 tcaaaataaa aatgagttct tataaaatat attcgcatta attgcaagaa aacatcattt
     4861 aatttttctt cagaattgct attcatactt gccctcctta ttttttatat tacttagtcg
     4921 gctaagcatt attttactat actgcatgca ttttttcaat atatttataa aaaattcata
     4981 taaatttcat ttattaaata tttttatatc atatagttat aattttaata aataatagta
     5041 agatttttaa ttagtcagct acttatattt tttattgtaa agcttaaaat aaaccttttc
     5101 atatattttc aaataaaaaa gttgtcccaa aataaatttt gaaacaacat catattcaac
     5161 agtatcttga ttatataaca ttacaatact ctacttatct tcatatccat ttacaacaac
     5221 gtctaacttc ccagtttcag gtgatataat tagaccatgt acaataatac catctggaat
     5281 taaaggatgc ccttttatta aatcaatact atcttttaca gattcttctg cagaatcgaa
     5341 tccatgtaac cattgttttg tatcaattcc agcactacga agtgtaagta ttacatcatt
     5401 agatattcct ctatctaaaa ttttaccaag taacttatct gtatcaacat tacacatacc
     5461 acatccatga tgccctacaa ccataacctc atctacatca aattcgtaaa ttgcaactaa
     5521 aacacttctc ataatacttc caaaaggatg cattatagta gctcctgcat tttttattag
     5581 tttcacatct ccatttttca aattcatagc cttaggcaaa agctcagtta atcttgtatc
     5641 catacaagat aagactacta tttttttatt tggatgtttt gatgtcacat attgttcata
     5701 ttctttgttc tctacaaatg atttattata ttctaatatc tcttcaagtt ttctcatatg
     5761 taatatcacc ctctttaaaa atttttttat tattatatca ct
//
LOCUS       DAELXX010000172         5772 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.174, whole genome shotgun sequence.
ACCESSION   DAELXX010000172 DAELXX010000000
VERSION     DAELXX010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5772
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.174"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            135..635
                     /locus_tag="KPH29_003477"
     CDS             135..635
                     /locus_tag="KPH29_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBG1469038.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIISGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            692..1678
                     /locus_tag="KPH29_003478"
     CDS             692..1678
                     /locus_tag="KPH29_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBG1469039.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSDKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            1728..2450
                     /locus_tag="KPH29_003479"
     CDS             1728..2450
                     /locus_tag="KPH29_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HBG1469040.1"
                     /translation="MENFNKNAKIIGIGVEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            2479..4413
                     /locus_tag="KPH29_003480"
     CDS             2479..4413
                     /locus_tag="KPH29_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG1469041.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFIEESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALDGGYFFPVRWIEYGKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNKYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKPEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            4406..5674
                     /locus_tag="KPH29_003481"
     CDS             4406..5674
                     /locus_tag="KPH29_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HBG1469042.1"
                     /translation="MSKNIYVKETYEWIRVGNGENELTEIEYEKLLKYLENNNDVLKS
                     NIIDIKYKKIRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPIT
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKKGIYFEYINKIENLNVMRGKILLSTYA
                     KEKGISPMKIRCEYDECSENNFLNQVLKKACISILCRINDNSIQGKIKKILSYFQNVD
                     LIYIDRKKLLGYKFYKNNDRFKDCYLLARLLLLNLSMDNSQNNQEAFSILFEINTLYE
                     EYIGILIKSIWDNSFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKNEYEIIID
                     TKWKAIEVDSNVFYRSSDIYQMYAYITAYENAKRCILLYPCIQKDKNYSSWKLSESFK
                     GKFIEAKTVRLDDIKNTKNDLKKIIFNYKF"
BASE COUNT         2432 a          432 c          978 g         1930 t
ORIGIN      
        1 taaaatgaat ttggaaaata gttcgctaaa ataatttgtt tttataaaat tactgtttaa
       61 gtttatagat tttgtataaa ataaagttat gttattaatt tttttagaaa taaaactttt
      121 actaggagac agatatgaga aaaaatagta ttaatatagc actaactgca gcaattacag
      181 caatctatgc agttttaaca ttatcattgg gatttataag ttatggacca atacaattta
      241 gggtagcaga gattatgatg cttttggcat ttttagataa aggatatata gtaggtctta
      301 cattaggttg ttttttagca aatgtaatag gaccatatgg tgtacctgat ataatttctg
      361 gaacattagc cacatttata agtgcaagta tgatttatat tactagaaag ttaggtaaac
      421 aaaataaaag tactttagtt atagcttctt tatggccaac tgttataaat tcgattgtgg
      481 ttggtctaat gttaaatgta ttctttgggc ttccattaat tctatctatg atacaggtag
      541 gttttggaga atttgtagta gtaacaattg taggagtacc atttttctgc tttttaatga
      601 aaaaatataa aaatgtcatt aatattaaat tttaaatagt gtatagtaat ttaattaatt
      661 gagagtatta ttagtgaaaa gaggtgttta tttgtcgaag gtaaaaagat tgactgatgt
      721 ttggatatat attaatgata agcaaaagtt tacaataaaa gagttgtcag aggaatttaa
      781 tctttcagct aaaacaatac aacgatattt aaaagaatta aataaaatgg gacttccaat
      841 tcaagcagaa caaggtagaa atggaggtta tagggtgcta aataatagct atatacctcc
      901 tgttatattt acagaaaaag aagttatgat tatactattt gcacttaagt cacttcagat
      961 ttataattat aagttaatgg aaatagaaat taattctata atgagaaaag taagttatga
     1021 acgtaaaagc agtataaaag taggtataga aaatattaaa agatacattg gctttgtagc
     1081 taatgatact aatgatggag attttaagag tagtaatgta actgagtttt ttagagcttc
     1141 ttcagaatct ctaattttaa atattaagta taagtgtggt aatcagattt tggaaaaaaa
     1201 cgtatgccct ataggtatat atttaaataa gggagcatgg ttttcaccag catatgattg
     1261 taaaagtgat aaaataatat tagttcatat agataatata attgatataa aaagaacagg
     1321 agaaagtaaa aaaatacata taaacttgga agaatggtta catgaagtat ataacaattt
     1381 atatggaaat agttcaacta ctaaaaaatt tggtgaaatt atttatctaa atgtattatt
     1441 aactaaagaa ggtgtttcta aaataaagga cagttcttgg aatttagata tggagctaaa
     1501 tgaagatggt agtggaataa ttagtacatt cataaaggtt gaagatatag attgttatgc
     1561 ttcgatttta ttttttattg gaaataatgc taaggttata gagccaaaat ttttgaataa
     1621 gctactttat gataaggcca atgagttaag atatttttat gaagaaaata tggtataata
     1681 tttagaaatg ttgaaattaa ataattatta aggagatata cgttataatg gaaaatttta
     1741 ataagaatgc taaaataatt ggtattggag ttgaaggtat taatataata aatgaagtag
     1801 aagaaaaaat aaaagcaaat atggatatag aaaaaataaa cataaatcaa gagattgaaa
     1861 aagaatatgt tagaagtctt ttagatggtg tagatatatt atttttgata tattcatctg
     1921 aagataaaca tattagagat attataaaag ctgtttcata tatgtcaaac gaaagaagag
     1981 ttctatctat aggaatggat tgttcagaga aagaaaataa agaagattta gagcttggga
     2041 gagagtttaa aatcaataat gatagtatat ttaaatttgt tgatttaatg aatatcatgg
     2101 tagagtctat ttctgattct tgcatgataa atatagatat tactgattta aaagaagcta
     2161 ttgtaggaga taaaggaatt aagtattctt ttgaagagtt tgaagataca aagtcatata
     2221 gcgagatagc tgacatttta tttgacagga tggaatatat tggtgaggaa tttataagta
     2281 agaaaggaat tgtatttgtt gaaggaagtc cagaattttc tataatggaa ttgaatgatt
     2341 taataagtaa tattcagagc aaggtagaag aaagctatga agtaatattt tctttatata
     2401 taaaggaaaa tttaaatgga aatattagag taggcttatt gtataattag tatattttag
     2461 atattaagga gagtgtatat gtctgaaaat ataagaggta cagagtattg gttaatggaa
     2521 gaattttttt atcaggaaac agacacacca cagattttta tagaagaaag cataattgca
     2581 cttggatttg atataaaaat aaatttaaaa aaagtattca gtatggggaa aaaatcagaa
     2641 aagtatatta atgagaattg taatgataaa gattttataa aaaagtttat gtcgattaaa
     2701 aaaggagatt attttttata taaagcatat caaaatatag aagaaaaagt taaatgtaat
     2761 gtgtctattg gcatagttga agaagatttt gaaaatggat atgaattaag tcatatttta
     2821 gggcatacac taccagtaaa atggatgcat aattttgata cagtttcaaa ttgtaaagta
     2881 tatacaagtg aattttataa attaaataat aatgatatta gttcatattt agaaattatt
     2941 aagtcacaag ataagaaatt attgagaaaa agaatagaag aaagtgaaaa taataaatgg
     3001 tatttaaaaa actattatta taaaaatgta gataagtgtg tagatgatga agaattattt
     3061 attcagccca ttaatctaga acaattagaa agcctaaaaa atataaagac agatgattat
     3121 attttattgt ataatagcga gaaagtaatt tgtgaaaatg accatacaat gaagagtcta
     3181 tttggcgtat gtcaagttat ggaagggttc gataaagaga aagtaagtgc tttggatggt
     3241 ggatattttt ttcctgttag atggatagag tatggaaagg agaatataga cggaaatgca
     3301 tgggttatat ttgaaaataa tagtatagtt gaaaagtatg taaaaagatt tactaataaa
     3361 atgccattaa atactatact ttatggacca ccaggaactg gtaaaacata caatacaaaa
     3421 cagatggttt acaatctagt aagtgaaacc agtctaagaa acaaatacta tacaggttac
     3481 ttagattata aaaacaatgt agaattttgt acatttcatc aatcatatgg atatgaggaa
     3541 tttatagagg gtcttaagtc tgatggtgaa ggcaatttta agccagaaga tgggatatta
     3601 aaggatatat ctatagaagc atgttataat gcattgaaat ttgaaaaaaa gatgagcttt
     3661 gagttggaaa aagatagctt gactttagaa actataaaag acaggaaaaa gaaattagta
     3721 ttagaaaata tagaatatag tgagagtttt aattttgaag aatcagaaaa ctatgtttta
     3781 ataatagatg aaattaatag agggaatgta tctaaaatat ttggtgagct aataacatta
     3841 ttggaagatg ataaaagact tactaaatca aatcaaacta ttgtcaaact accatattca
     3901 aaggaaaaat tttctctacc tccaaatttg tatatagttg gaactatgaa cacatcagat
     3961 aaatcaatag cacttttaga tatagcttta agaagaaggt ttaattttga agaaattatg
     4021 ccagattatg atttattgtc tattgtagat ggtatagata ttagaaaaat gctatataca
     4081 atcaataaaa ggatagagtt tttatatgat agagaccatg ttattggaca agcttatcta
     4141 cttgctgtag aaaatattaa tgatatagta gtagtattta gaagtaaaat aataccactt
     4201 cttcaagagt atttttatta tgattcagaa aaagtaggtt tgattttagg aggaattgga
     4261 agaaatgaaa aagataaata cattgtttat aaagaaaaac ttatagcttc tgagttattt
     4321 aaaaataata atgcctcaat aatggagagt aaagttaact attgtataaa acaaaacata
     4381 tctagtgagg agctaaaaaa tatatatgag taaaaatata tatgttaagg aaacctatga
     4441 atggataagg gtaggtaatg gtgaaaatga gcttacagaa attgaatatg aaaaattgct
     4501 taaatatcta gagaacaata atgatgtatt aaaaagtaat ataatagata ttaaatacaa
     4561 aaaaattaga tttataaact atgtaggcat catatgtttt gaaaatgtaa tacttgaaat
     4621 acttcctaaa ttgtcattaa gtgataatct tgtaaaagat agagagatac tattacagat
     4681 gctttctata tgtaataaaa ttccaattac aatgaatgaa aaaataagat tgagtttgaa
     4741 aaattataat ctcttaaatt tttttgttat gtatttcatt gagagtatgc aaacacagat
     4801 gaaaaaaggt atttattttg aatatataaa taaaatagag aacttaaatg ttatgagggg
     4861 taaaatatta ttaagtacat atgcaaaaga aaaaggcatt tctccaatga aaataagatg
     4921 tgaatatgat gagtgtagtg aaaataattt cttgaatcaa gttcttaaaa aagcatgtat
     4981 atctatttta tgtagaataa atgacaattc aattcaaggc aagattaaaa aaattttaag
     5041 ctattttcaa aatgtagatt taatttatat agatagaaaa aaactgttgg gttataaatt
     5101 ttataaaaac aatgatagat ttaaagattg ttatttacta gctagacttc tacttttaaa
     5161 tctatctatg gataatagtc aaaataatca agaggcattt tctatattat ttgaaataaa
     5221 tactttgtat gaagaatata taggaatttt aatcaaaagt atatgggaca attcatttag
     5281 agaaacatat atacaagata agagtaaatt tcttttgaaa aatgaacaaa caggtaagaa
     5341 aaactttaat ttaagaccgg atatagtttt atatgactta aaaaatgaat atgaaattat
     5401 aattgacact aaatggaaag ctattgaagt agattcaaat gttttttata ggtcaagtga
     5461 tatataccag atgtatgcat atataacagc atatgaaaat gcaaaaagat gtatattatt
     5521 atatccttgt attcaaaaag ataagaatta tagttcctgg aaattatcag aatcatttaa
     5581 gggtaagttt atagaagcaa aaactgtgag gttagatgat ataaaaaaca caaaaaatga
     5641 tttaaaaaag ataattttta attataaatt ttaaaaataa gaattttaaa atttataaaa
     5701 gtaattttat ataggtataa aaattacgag tagtataaaa aatttaataa gaacttaaat
     5761 taaaaatttt aa
//
LOCUS       DAELXX010000173         5744 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.175, whole genome shotgun sequence.
ACCESSION   DAELXX010000173 DAELXX010000000
VERSION     DAELXX010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.175"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            244..1512
                     /locus_tag="KPH29_003482"
     CDS             244..1512
                     /locus_tag="KPH29_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBG1469043.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            1707..2495
                     /locus_tag="KPH29_003483"
     CDS             1707..2495
                     /locus_tag="KPH29_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBG1469044.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSLRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            2495..2944
                     /locus_tag="KPH29_003484"
     CDS             2495..2944
                     /locus_tag="KPH29_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBG1469045.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            2948..5212
                     /locus_tag="KPH29_003485"
     CDS             2948..5212
                     /locus_tag="KPH29_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG1469046.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKNALVVKILRSPHAY
                     AKIKDIDTSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVE
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGSMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            5352..5663
                     /locus_tag="KPH29_003486"
     CDS             5352..5663
                     /locus_tag="KPH29_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1469047.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
BASE COUNT         2199 a          615 c         1094 g         1836 t
ORIGIN      
        1 tatctaaaaa gatatggata taaaaaatat taatatatat atactataaa cttgacaaaa
       61 tcaacctttt aataactagc aaaaaatagc aaaattatat aagcgttatt taattatcaa
      121 aaatgaaagc taaattgcat gtcctaaaaa aaatgattga taacatttat ttgtgtgcta
      181 taattatata gtaaaacaat aagtatattg cataatgtat gcaaatcggg aggagagacg
      241 gacatggaag catctataca cacaattgcc agcaataatt tattgataat ttttgctatt
      301 gtaagtataa ctggaataat ttgtagtaag cttagtgaaa taataaaggt tccagatgta
      361 gtgttatatt tacttgtagg tattttaata ggaccatcat ttttaaaatt tatagatatt
      421 cgtggatttc aaatagaaaa tcaactaata cttacatttg gttctgcatt tattttatat
      481 ttaggtggaa aagaaataag tttaaaagta ttgagaaatg tgaagatatc tgtattctta
      541 ttagctactt taggagttgt gatatctgct tttataatgc aacaagtaat aggatttact
      601 tttgggataa gtgcaatgac tgctttacta gcaggaacta taatagcatc aacagaccca
      661 gccactcttg tacctatatt taatcaggta aagataaagg atagggtaaa acaaactgta
      721 ataagtgaat cagcttttaa tgatgcaaca ggagctatac taacatcagc agtgttggct
      781 gtaattttgt caaataaatt ttcattaggt gaaaatattt atgaactggg gacaatggtt
      841 attgtcggag ttttagttgg tttaattaca ggaatactgc ttttgaaatt agttaatgat
      901 aaaccttatg gaatatttaa agattttgca ccaataatat ctgttatttc agttgtagtt
      961 gcatatgaat tatctacaaa attaggtgga agtggatata tgtcttgctt tattgtaggt
     1021 ataataactg gtaacaagaa aaactttaaa atttggttaa gtcaaaaatc ttatgatgca
     1081 gatttttatg ttgctgaaac tttaggtact atttgtcgta tggcaatatt tataatattg
     1141 ggtagccaag ttaatttggc tcagttaagt aaatatttta tgccatctgt aattacagta
     1201 ttagtattaa tgtttatagc aagaccactt tgcgttttag tttgtacact tgtagacagg
     1261 gaagcaaaat ggagtaaaag tgaaatatta tttatgatgt gggtaaggga aacaggcgta
     1321 ataccagcag cactttgtgg tattatttca gcaatgaaag ttcctggata cgaagtgatt
     1381 tcatctgtag tatttatgac tatattaatt acattagtaa tacaaggaag tactacaaaa
     1441 ttagtagcta aaaaacttgg attattagaa gaagaagtga taaatataag tgaaaaagta
     1501 tcaacttttt aaagttttgc tttaaaatag tacctgttaa tggctatttt aatattgtct
     1561 aatgatgatt attgaaatag tcattttttt atgtataagt aatttacaga atttacatat
     1621 tagacatatt tattatcaaa atttgttgaa gctatgatat aattagttgt atttaaaata
     1681 agaatagcaa ataagggagg aaatttatgt ttacggttat ggatattgta cagccagata
     1741 cagtagaaga agcgtactca attttaaata aaagaaaaac taaccaagta attggtggaa
     1801 gtgctttttt aagaatgggg aaaaaaagaa taggaacagg aatagaatta tctaaattaa
     1861 atttagatta tataaaagag tatgaagatt acgttgagat tggttctatg actactttta
     1921 gaacattaga aacaagttcc ataataaaaa ataattttgg aagaataata gaagattcag
     1981 ttaaaaatat aataggagtt cagtttagaa atgtagtaac agttggtgca acagtatttt
     2041 ctaaatatgg tttttctgac ttgatagtag cattactttc attagatacg gaagtagaat
     2101 tatataatat tgggagaatt agtcttgaag agtttctaaa tagagattat gagaaagatt
     2161 tacttattaa gatatacata aagaagacaa ataagaatgc ttcttataaa tcacttagaa
     2221 atgcaaagag tgattatcca attctaaatg tatctgtatc aaaacatatg gaacaattta
     2281 agttatgtgt aggagcaaga ccgcagaaag ctacgattgc aaaacaagct agtgaatttc
     2341 tatctaacaa tgaaatcaat gaaattaata tagataaagc agtagaaata gcatcagaag
     2401 aactaacttt tggttctaac atgagagcat ctagggagta tagaaaagct atgagtaaag
     2461 tacttttaaa aagagctata atggaggtta tttaatgtta gtaaagatta atataaatgg
     2521 gaagaaaaaa agtgttgata tagaaccaga agaatatctg gttgatactt taagaaaagt
     2581 tggtaattta agtgtgaaaa gaggttgtga cacaggctgt tgtggtttat gtacagtttt
     2641 aattgacaag aaacctacat tatcctgtgc aacactgaca ttgagagctt tagataaaga
     2701 aataactaca attgaaggtt tagaaaatga agttaaagaa tttagtgaag ttttggtaaa
     2761 agaaggagct gagcaatgtg gtttctgttc tcctggcttt atacttactg tacttgcaat
     2821 gaaggatgag cttgaaaatc ctacagatga tgaaataaag cattatctta caggaaatct
     2881 atgtagatgt acagggtata tgggacagtt aagagctatc aaaacttatc tggaggtaaa
     2941 atagaacatg aatatagttg gaaaaggaat caagaaaata gatggaatga gtatcacaac
     3001 aggaaagcct gtatatacag atgatttatc agcaaaaaat gctttggtgg taaagatact
     3061 tagaagtcct cacgcttatg caaaaataaa agatatagat acatcaaagg cagaaatggt
     3121 tgatggtgtt gaatgtgttt taacatataa agatgtacca aaatctcgat ttacacttgc
     3181 tggtcaaaca tatccagagc catctccata tgatagatta attcttgaag atgttgtaag
     3241 atatgtaggt gacgaagtag ctatagtggc agctatagat gaaaaaactg caattaaagc
     3301 aatgaatctt atcaaagtaa agtatgaagt acttacacct gttttggatt ttgaagaggc
     3361 aataggaaat aaaactatgg tacatgatga agatagacat tgtaactttg atataggtat
     3421 gcaaagagaa aaaaatatag tatcccatca tgattataca aaaggtgatg tagaaaaagt
     3481 attaagtaat tgtgatgtag tgattgaaga aacttattat actcaagctc aagcacatgc
     3541 aatgatggag acgtataggg catacaatta tttagaccat atgggaagat tggttgtagt
     3601 aagttcaact caaataccat ttcatgtaaa aagacattta tctcgtgcac tagaaatacc
     3661 agctagcaaa attagggtta taaaacctag aattggtggc ggatttggtg gtaaacaaac
     3721 tgcgtgtgtt gagatttttt ctgcaattgt tactttaaaa acagggaaac cagctaaaat
     3781 aatttatgat agaaaagaaa cttttaattg ttcaacaagt agacatgcaa tgagattaaa
     3841 agttagacta ggtgctacaa atgatggcat aataaaggca atagatataa aagcactttc
     3901 tgatacagga gcatatggag agcatgcttc tacaactatt ggtttggttg gagaaaaaac
     3961 tttaccaatg tataataaag ttgaagcaac aagatttatt gcagatgtgg tatatactaa
     4021 taagatgcca gcaggagcac ttagagggta tggagcatca caaggatgtt ttgctgttga
     4081 atcaactata aatatgcttg ctaataaatt aaaaatggac ccaacagatt taaggttaaa
     4141 aaatgtggta acagaaggtg aaactacatt tgcatatggt aaaactttaa atagtgtcga
     4201 tttaataaaa tgtataaata aaggcaaaga acttattgga tggaatgaaa aatatccttg
     4261 taaacatctt gataatggaa aaattagaag tgtgggtatg gcaattacta tgcaaggttc
     4321 tggtatacaa ggtcttgata agtcttcagc tgaaataaaa ttgaatgact gtggagatta
     4381 tactcttatg ataggttcta ctgatatggg tacaggaagt gatacgattt tagctcaaat
     4441 ggctgctgag gttcttgata caagcattga aaatataaca gttatatcag ctgatactga
     4501 cttagttcca tatgacccag gttcatatgc ttcaagtact acttatgtaa caggtatggc
     4561 agtagttaag gcagctcaag acttaagaga aaaaataatt tctgctggag caactaaact
     4621 aggtgttgag aaggatgaag tagaatttga tggtataaaa ttaactagta aaaaaggtag
     4681 cactttatta ttcgatatag cagttgattc tacaattggt ccagatacta atcaattaat
     4741 aggtcttgct tctcatggta gcccagtatc tcctccacct tttatagcag gttttgcaga
     4801 aactgaaatt gacacagaaa ctggaaaagt tgatttagta aattatgtag ctgtagtaga
     4861 ttgtggaacg gttataaata aaaatctagc taagatacaa gttgaaggtg gaattgttca
     4921 agggattgga atggcattat ttgaagaagt tagatacaca gaaaaaggaa gcatggacac
     4981 aaatacattt atgcaataca agattccagc tagatgtgat attggaaatg ttaaagtaga
     5041 ttttgttgaa acttatgaac caactggacc atttggtgct aaatctgttg gagaagttgt
     5101 tataaataca cctttaccag caatacaaga agctgtgttt aatggaattg gtgtaagaat
     5161 aaatacactc cctattacac ctgaaaaagt ttttatggaa atgaataaat aattatttat
     5221 gaaaatttag actagtttta catgttaaac aaccagttat gtcatacctg ttgaaagata
     5281 tttaataagt agttattata atattatgaa ttttacttat aaaatacaaa attaaataag
     5341 gggatgatgt tttggctggt aatctaaata atatgagagc agtaaataat tttagaggag
     5401 ataagaacat tttagaatgt ttagtcagct ttgagggtcg ttcaataagt cagagaaaag
     5461 taagggtatt ttttaaagaa aaacaaaatc aaatagaaat tgattttgca gaagaggaaa
     5521 tttctaaatt ggttgaaaat gttgttttaa atacatcata tcaagaaatg ttatatgatg
     5581 aaatagagaa acaactggaa attgattgta taggtacttg gatgatatta tctaaattaa
     5641 aagatggtag tagagttcac tgattttaaa ataaattatt agagcctaat tttaaggctc
     5701 ttttttgctg tttttaatta acaatagaaa atttatcttt agtt
//
LOCUS       DAELXX010000174         5488 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.176, whole genome shotgun sequence.
ACCESSION   DAELXX010000174 DAELXX010000000
VERSION     DAELXX010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5488
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.176"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..717)
                     /locus_tag="KPH29_003487"
     CDS             complement(<1..717)
                     /locus_tag="KPH29_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1469048.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLIL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRKLNEDNLENIIKLKKE"
     gene            complement(686..1699)
                     /locus_tag="KPH29_003488"
     CDS             complement(686..1699)
                     /locus_tag="KPH29_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG1469049.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADLGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKDNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            complement(1721..2491)
                     /locus_tag="KPH29_003489"
     CDS             complement(1721..2491)
                     /locus_tag="KPH29_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1469050.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSMHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            complement(2466..2756)
                     /locus_tag="KPH29_003490"
     CDS             complement(2466..2756)
                     /locus_tag="KPH29_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HBG1469051.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(2887..2986)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     regulatory      3606..3695
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            3748..3924
                     /locus_tag="KPH29_003491"
     CDS             3748..3924
                     /locus_tag="KPH29_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1469052.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     gene            complement(4039..4395)
                     /locus_tag="KPH29_003492"
     CDS             complement(4039..4395)
                     /locus_tag="KPH29_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1469053.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKDVVHISHKILSQELKYLERDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
     gene            5126..>5488
                     /locus_tag="KPH29_003493"
     CDS             5126..>5488
                     /locus_tag="KPH29_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1469054.1"
                     /translation="MIAVKKAYKFRIYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KNDKETFTYKQCSSDLTNLKKELNWLKEPDKFSLQNALKDLENAYNKFFKEKAGFPKF
                     KSKKTNRFSYKTNFTNGNI"
BASE COUNT         1924 a          867 c          663 g         2034 t
ORIGIN      
        1 ttctttttta agtttaatga tattttcaag gttatcttca tttaactttc tactatctat
       61 aacaaattct ttctttacta ttgatggttt ttttgaaata acataaattc tgtttgatag
      121 cattatagct tcgtcaatat catgagttat aagaagtatt gttgaattta cttttttctt
      181 aacatctaaa agccattttt gcatggatgt tttagttaat gaatcaagtg ccccaaaagg
      241 ctcatctaaa agcattatgt cattagaatt tataaaagtt ctcaagaagt tagctctctg
      301 tctcattcct ccagaaagtt ctgaaggata tttatcttca tatccgctta atccaaacac
      361 ctcaaaataa ggttttacca ttgacctagc tttactcttt ttttcttttt ttaaaattag
      421 tggtagtgac acattatcta ttatagtttt atatggcaag agtaaatctt tttgatacat
      481 ataactatat ttaccattta tattcacttt gccactatca taatctgtaa gtttagttat
      541 aatattaaaa atagtacttt ttccacatcc acttggccct aatatagaaa caaactcacc
      601 ttcataaaca tcaatactta tatcttttaa aacttcaata tctttataag tttttgatac
      661 attttctata gatactttta cttttttatt gtggtaaaaa ttcatttgtg taagcatcac
      721 ttgccttcat atccttatct attaatttat attcttttaa gaatgatgta tagttgttcc
      781 atacactatc tttcatttct ccccatcgtg gtgcatcatc tttgtacttg cttgctaaat
      841 attcttgact ctttaaagct agtttttcat ctacttcagg tgcatgtttt actaaaattt
      901 ttgctgattc ttctggattt tttattgcat attcataacc ttttgttgta gcttttaaga
      961 attttttagc aagctctgga ttatctttaa gcacagtttc attagaaatt ataagtggtg
     1021 tataataatc caatctctca tctaaatctt ttactggtat aaagtttaag tcatatccaa
     1081 ttaaatcagc tttaactgca tcccaacctt caaatatcca tgcaaaatct acagttttca
     1141 tagctgcaaa gaaatcatct tgaccagtat taactatctt taacttatta aaatcagctc
     1201 catttttctt cataactgct ttaaaaacag cttcttctga tggagagccc caaccaccat
     1261 aagtttttcc ttcaaaatct ttagctgttg ttatattctt ttcttttggt gatgcaaacc
     1321 cagaagtatt atgttgtata acagttgcta cagcttttat tggaagtgga tcttcactag
     1381 tctttgcata agtaacttgt tcttgatagc ttatacctaa gtcagctttt cctgttgcta
     1441 ctaaagtttc tgctccactt tcaggtggtt gtactatttc aacatccaaa ccttcttctt
     1501 tatagtaacc cttatcaagt gctacaaata atccagtatg atttgtattt ggtgtccaat
     1561 caagcaccat agttactttt ttagaagaag tatctttatc attgtttttg tctttattgt
     1621 tatttgaaga acatccagtt attgttgcca atgtaaaaac acttactaaa cctaatattc
     1681 ctaacttttt aaaattcata attgcctccc tcaaatacct ttaatcaatt aatctatgtc
     1741 ttataacaac tttttttaca gcctgtacaa ttcctacaaa cattaaacta aatataacta
     1801 ctaatatagt agatgcaaat actttatcaa gtgcatatgc actttttgac ctaagcatat
     1861 aaacaccaag tcctttcgtt ccacctagcc attctgcaac agttgctgcc attacagcat
     1921 acgttgcaga tattttaagt ccagaaaaaa atttatccat tgccatagga aatttaaggt
     1981 gaatgaatgt attaacttta tttgagtcca tagtcttaaa taaatttaaa taatctttat
     2041 caatattctc aatgccatct acaaaactta ttaatatagg aaaaaaacaa gtaagcatta
     2101 ccatcaatac ttttggcatt gttccaaatc caaaccatat cataaaaagt ggtgctataa
     2161 ttattgtagg tatcatctgt gtaaccaaca taataggata aagacatttt tttattattg
     2221 atacaaaatc catcaatatt ccaattacca atgatattat aattgcgact ataaaaccaa
     2281 gtattgcttc tcctatagta accacagcat gcatggataa attttgataa tctttaaaaa
     2341 acacattaat tatatctact ggtgtgggta gtatatactg aggaacttct aaaactccta
     2401 ccatagattg ccaaagaata agtataaaca aaaatgttgc tattggatat atactatctt
     2461 tgactttatc tatgatttct aagtttttca tctatagtca gacctccagc tttttcatct
     2521 actctagcta ctgttatgac tctttcacat ccaagattaa aagggatttt atgtaccttt
     2581 tttaataatt caaaaacttc atcaaaatca ccttcaacag tagtgctcat tgctcctatt
     2641 tcacatttaa gaccactttt ttcaatttca gcaatacaag catctactac tttatacatt
     2701 tcttcttcac ctttgtaagg tcttagtggc attactgcta tgtctgcgat taccatttaa
     2761 atcactcctt taaaaataat taatacctac taggtatatt ttgtttttta ttaaaaaatt
     2821 ttattactaa aaagatatta tatttaaata aaagaaaagt cttatccaaa ataggataag
     2881 acttattttt atcttaactg gtctccctac actggcatta tccaaacagg ttcaaagggt
     2941 cgagataaaa tctcctctca gcacattagc tccccgaaat atattatatc ttttctactt
     3001 gattatacta cacttatcat attttaacaa attttttatt tatattagat attttatccg
     3061 ttcaaaaata tctaatatac attgtacatt attacaccaa ataaattatc ttaaaatggt
     3121 aatttatcaa catcttccaa aactagatta actgaatttt ctagttttta ttttgctatt
     3181 tcaagcatat ttggatgtat atcaattcca tacaaatatt gttctaaatt tctgttttca
     3241 ctaatctaat ttgcaatcgt tacagattac agattagaaa tagcttttag actctactta
     3301 tctttatcta caaaaaatcc ttcaataaat tcagtaataa tattcagtaa tctaaaaata
     3361 aacctctatc tggattttta agatacaagc cttttaggaa gtatcttttg tgataatata
     3421 aaaattactc gtcattatca tcaaaatcct cagcaaccaa cattttagat aagaatcaaa
     3481 ctctataata taattatttt atccaattta tttttgagaa ataaaatatt tttttaattt
     3541 ttaaattttt taatatgcac cttgaaaaaa gaaacgtttt cctatattat ggtaatagta
     3601 gaataaaact aaataggcaa aactagagaa atttagtgac gcaaagctat agggactaag
     3661 acttatgaaa tgagttatgt cagccagttg ccaaagagat attttctttt tgtttttatg
     3721 aatgtttttt agggggatat atgatatatg tttatggata aaatggatag atgtacacat
     3781 atactaactg catatatatg cagttcacgc gattactgca attttataga tacacaatta
     3841 aatgatttta tactagaata tggagagaat gtggtagaat cttgtttgca tcaagtgatg
     3901 gtattggtaa gcagatataa ttaaagaaat tttattttct agaaaaccct ttttagtttt
     3961 atctacgttt tataattcat taaagaacta atcaaaataa gcttcattat atatttttca
     4021 tgaagtaaac agttgagttt atctcatata ctgtttaccc caattacata attctaataa
     4081 tatagattcc aatgatttac cacgtttagt agacaaatat tcaacttttg gaggaatagt
     4141 cggataagaa attctcttaa taagtccatc tctttcaaga tactttaatt cttgtgacag
     4201 tatcttatga cttatatgaa caacatcttt ttttaattga ctgtaacgta ttggctcttg
     4261 ttttaaaata tgccataaaa taattgccct ccatttactt cctatcacag tcatggtaaa
     4321 accaattgaa caattaaaat ctttttgtct ttcttcaata tttggaaatt cttcaaaatt
     4381 attatctata tccatctcaa tcctctcttt caaaatagta agtatctaac taaaaagtgc
     4441 attcttgttt agcgtactaa ttcgtaatat aatcttgatt aaaaaatagc tacgcacact
     4501 cgtgacttta gtcatgagtt agtagctaaa atagtcaaca tatagggaaa cctatatgta
     4561 gagataggat agaacctatc caatactact tgaattgcta aaaaccccta aagctaacca
     4621 aactaaaaca taaggatgaa ataaacctaa gtgtgaaagt tacgaaagta gaaaaaattg
     4681 gttagatggt ataaggttaa accctaagta ctttaaaatg ggtaatcagc aggtaaactc
     4741 cgaataggag aaacttcaac gactattcct cttgagggaa gtacactaca agttatcggt
     4801 agtggaagtg ggtagacctt aacggataat gccgaaggat aagatatagt ctgtgcttat
     4861 gtgaaagtgt aagaagttca taagagaact gcataggtag tagcgaactt atgtgaacga
     4921 cagcctcaaa aatgataaaa gttaagactt acggttctta tatatgttat ttaaaattaa
     4981 atataaaacc ttctaaataa ttgttttaaa taattacatt tgttaatatt atgataggtg
     5041 attaataaat tgttcaccac agtattatat aatatattaa atattaaaat cattaaaaag
     5101 tgtatcaaaa aacaattaaa gtgaggtgat agcagtgaaa aaagcttata agtttagaat
     5161 atatccaaat aaaaaacaac aagagttaat taacaaaact tttggatgtt gtagatttgt
     5221 atataataaa tatcttgcta aaagaataga tgtttataaa aacgataaag aaacttttac
     5281 ttataaacag tgtagttctg atttgaccaa tttaaaaaaa gaattaaatt ggcttaaaga
     5341 acctgataaa ttttcactac aaaacgcctt aaaagattta gaaaacgcat ataataaatt
     5401 ctttaaagaa aaagcaggat ttcctaaatt taaatcaaag aaaactaata gattttcata
     5461 taaaactaac tttacaaatg gaaatatc
//
LOCUS       DAELXX010000175         5450 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.177, whole genome shotgun sequence.
ACCESSION   DAELXX010000175 DAELXX010000000
VERSION     DAELXX010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5450
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.177"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            555..1415
                     /locus_tag="KPH29_003494"
     CDS             555..1415
                     /locus_tag="KPH29_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG1469055.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKALNIKSSDYLSLTLLDHLEF
                     LLKRCEKNQFIKSPLVWNVKRFYPKHFEIGMHALKLIGDSKGINLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSD
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
     gene            1405..3276
                     /locus_tag="KPH29_003495"
     CDS             1405..3276
                     /locus_tag="KPH29_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG1469056.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRLTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASILALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQATTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSVTLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEASNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            3296..4750
                     /locus_tag="KPH29_003496"
     CDS             3296..4750
                     /locus_tag="KPH29_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBG1469057.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYETPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLMDLYLKYSETVI
                     RRFDDKVKYWITFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKVGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLSKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
BASE COUNT         2115 a          551 c          965 g         1819 t
ORIGIN      
        1 aattaaattt aagtatttat atattagtat atcttactag tgtttaaata aaactaaatt
       61 taagtattta tatattagta tatcttaata gcatttaaat aaaactaaat ttgaagcttt
      121 acctattaat atatcttaat agtgattaaa taaaactaaa cttaagaatt tatttattaa
      181 catacagttc attatattta aaatcattac tatgataatt cataatagta aaatagataa
      241 tggttcagac aatattgtaa atattagtga cagtaaaata gtatcaattt agtatataaa
      301 ctttgccaga taatcgcata acagagcttg tagttataga ataaaaaaat atctaggaaa
      361 ccgttgacag atttatatgg atagtataac atatagaata tgaaattaaa caaaaaagaa
      421 ttttaattat ggcgacgtag tcataatttt gagtatgtta ctgattagca ggcagaactc
      481 aagtatatat gtgaaaaata gagggatact ctatacatat atgcttgagt tctttttatt
      541 gaaaggagga atatatgaaa ataattcaga ttttaaataa taatgtagcc ttagtaaaaa
      601 gaggtaaaaa tgaaattatt gtatttgtta aaggaattgg ctttaaaaaa agagtgggac
      661 aagtaataaa tgaaaatgaa atagagagaa catatatact agattcatac gatatgctag
      721 aacactttag ttatctacta caaaactcag actccaataa tatagttctt attaatgaaa
      781 taattgaata tggtgagaaa gctttaaata taaagtcaag tgattatcta agcttaacct
      841 tactagacca tttagagttt ttacttaaaa gatgtgaaaa gaatcaattt ataaaaagtc
      901 ctttggtatg gaatgttaaa agattttatc caaagcattt tgaaattgga atgcatgcac
      961 taaaattaat tggtgatagt aagggaatta atcttcctga tgatgaggca gtatcaatag
     1021 cacttcattt tgtaaatatg gaggtaaata aagagtctca tgatagtaca attgtagagt
     1081 tgagaacttt agcagatata gtttctataa taaaatatca ttttaatgta gagctagatg
     1141 aaacttcaac aaattatatg agatttacta ctcatctaca gtactttatc caaagaatta
     1201 tgtcagatga agcttcaata gatgaagata attctataga tttatataaa caagtaagtc
     1261 aactttatcc aaagtcattt ggaagtgttc aaaagataag aaaatatata aaatcacaat
     1321 ttaatgtcga tatatctata agtgaagaaa cttatttaat gttgcatata aatagggtga
     1381 ctcaaagact ggaggcaaaa tcagatgaaa tataaagatt taaatgaaaa tataataaaa
     1441 cttatagggg gaaaagaaaa tataaagtcg gtagcgcatt gtgttactcg tttaagactt
     1501 actttaaatg atagaagtat cgctcaaaca gaagaaatta aaaaattaaa aggagtaatt
     1561 gatgtagttt ctaatgatgt tgcttatcaa attataatag gtactcatgt tgtagatgta
     1621 tataacgagt ttatgtctat gataggttta agttcaagtg aatcaagtat aaaaagtgaa
     1681 agaaaaaagg gaattagagg tatcaaaggt atatttaatt ccatctttgt ggtagtctca
     1741 gagactatga cttcagtaat tgaagtacta cttgcagctg gtatactcgc aagtatatta
     1801 gcacttttaa atatgtctgg catacttcag agtgatagcc ctacatatca aatattagat
     1861 acattaagaa gtgctacatt tcacttctta ccagtattaa ttgcaatagc atcagcaaaa
     1921 agacttaatg taaatcccta tatagcagta gtgttagcag ttacgttatt atcgtcatca
     1981 atagatggtg tagaaggatt aagtttattt ggtataaatt tacaggctac aacttatgca
     2041 aacagtttta ttccaatact tttaggagta tgggcaatgg gaattataat tgaaggtttg
     2101 aaaaaaatat tacctaaagc actacactat tttttagttc cagtgttatc tttaataata
     2161 accctatcag ttacattaac tgtatttgga cctattggta tgtggattgg tgatggatta
     2221 aactatgcat gtatgttttt aattgataca ttgggtaatt ggagtgttgt tgcactgtat
     2281 gcagctttac aaccactctt aatagttttt ggtgcagcaa actttactat gcctatatct
     2341 ttaaattctg tgacaacatt ggggtatgac ccaattttta tgggcgctgc aacaatatct
     2401 gatattgcag tttgtggtgc aatgtttgga tatttcttaa aaactaggaa taaagaacaa
     2461 aaacaattat ttgggacagt aagttttagt gcacttatgg gagttacaga gccttcagtg
     2521 tttggtgtgt ttatgaaata tagaagacca tttgtagcag taatgatagg tggaggtata
     2581 ggtggtttaa tagcaggttt agctcaagtt aaaacatatg gaatggtttg gggattagct
     2641 gcattaccaa catatcttgt tggaggagag gcatctaact ttacatttat gataataagt
     2701 gtagtagtat catttgtagc agcaacagca gcttcctatt tactaggaat accttcagat
     2761 gaaaaagaag aagttaaggg tgataatgaa aaagaattag aactaataat caacgagaac
     2821 aattcaagaa ctattagtat tggaaatgtt gcaaaaggtc aggtaattgc acttggggaa
     2881 gttaatgaca aagcattttc ctctggagca ttaggtaaag gggttggagt tatacctagt
     2941 gaatcaaaat caactgttat atctcctgta gatggaacta taactatagt atttccaact
     3001 aaacatgcat atggtatagt gactgatgaa ggattagaaa tattaattca tattggtata
     3061 gatactgtaa acttagatgg aaaattcttt gaaagtcttg taacacaaaa tcaaagggtt
     3121 aaaaaaggag atgctttagc agtatttgaa tctgataaaa ttatagagga aggctttgat
     3181 tctactataa taactgtagt tacaaatact agcgattatt tagatgtaat ctcatcaaat
     3241 gatgaatcag aagaattatt aactgtaata gtataaattt taggagggaa aattcatgaa
     3301 tattaaagat acatttttct ggggaggaag tattgcagcc catcaatgtg aaggttcatg
     3361 ggatagtgat aacaaaggac ctgcaattat ggattttgta actaagggct cttatgaaac
     3421 gccaagagta attactgata aaatagaaga aaaattggat tatccatctc ataatggaat
     3481 agatttttat aatagatata aagaagatat agctttattt aaagaaatgg gatttagtgc
     3541 attaagaata tctatagatt ggtcaagaat ttttccaaat ggagatgatg aaaatcctaa
     3601 tgaattaggg attaaatact atgaaggtct gatagataca ttaatagaaa ataatataga
     3661 acctattgta acactatatc attttgaatt acctatgaat ttagtacata aatatggttc
     3721 atggaataac agaaaactta tggatttgta tttaaagtac agtgagactg taataagaag
     3781 gtttgatgat aaagtaaagt actggattac atttaatgag atgaaccata ttgaccctca
     3841 aacagagcac tcggatattt ttacttatat gatagcaggt attaagtata gtgaaataga
     3901 aaataaggtt caaacattgg caaatgtagg gtataatatg actttggcgg gtgtaaaggt
     3961 tgcaaagttg gctagagaaa tcaatcccaa caataaagtt ggatgtgtat ttggactaaa
     4021 tccaatttat ccatacaatt gtaatcctag taatgtgtta aatgcatttt tagataatga
     4081 tcgtgattat tatcaaattg atgcaatgtg caatgggaag tttccccaat ataaattaaa
     4141 agaatatgaa aaaaataata taactattga agtgactgat gaagacaaaa aagcatttaa
     4201 agaaggtaag ttagatttta taggtcttaa ttattatttt tcaagtgtat ctactccaaa
     4261 agaaaattta tcaggtgata aatctttatt tggtgggatt caaaatccat atttggaaca
     4321 aagtaagtgg ggatgggcaa tagaccctat aggtttaaga tttaccttga actatattta
     4381 cagaaagtat caattaccta tcttaattac agaaaatggt ttaggggcat atgataaaat
     4441 cgaagaagat ggaactataa atgatgatta tagaattgaa tatttaagta agcatctatc
     4501 tcaaatgaag tctgcaattg aagaggataa tgttgaatgt tttggatatt tgatgtgggg
     4561 accaattgat ttagtcagtg caactacagg tcagatgagt aagagatatg gatttatata
     4621 tgttgactta gatgatgaag gcaaaggaag ttttaagaga tataagaaaa aatcatttga
     4681 ttggtacaaa gaagtaataa aatctaatgg agaagtttta gatttttcta cggatatagt
     4741 agtagggtag taataagata aatagaagtt aggaactaat gttaattagt gtctagtaaa
     4801 gcatttaaaa tagagtatag ggtctttaaa gtaaagtgtg tattcttatt aaaagtaatg
     4861 gcaactaaaa ttactataaa aaggagaata cacatttttt atgtttatac ttaagtaaat
     4921 atttagacat attcatagta taatttactc attatataaa aatagtatga tttactcact
     4981 atataacttt ataaattttc tagaaaaatg tcagaatata ttgacaaaaa aaagatttga
     5041 aattataatc tatattgaga atagtttgac aatataaaaa caagtatttt tacaaatagt
     5101 gaaattattt ttattgtcaa tttaagaata atttaggact actataaaga tgaaggtata
     5161 gatgtcatta tatgataaat catacttgta tagaaagaat tttaaagtat gcttatcatt
     5221 gatttaatat aataaataat ttttgtttct agtaatagtt cataggaata agatatctaa
     5281 attcgcttag aaatagtcta atttacaaag gaggggatgc actagtagat aaaattttta
     5341 tataaagaag acttaaagtg gagtatattc tgataatatt ggtttaatgg ttatattatt
     5401 atttcgtttt agatatttta agaataattt aagacgtttg taaaaatgta
//
LOCUS       DAELXX010000176         5359 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.178, whole genome shotgun sequence.
ACCESSION   DAELXX010000176 DAELXX010000000
VERSION     DAELXX010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5359
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.178"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            91..564
                     /locus_tag="KPH29_003497"
     CDS             91..564
                     /locus_tag="KPH29_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG1469058.1"
                     /translation="MFKEYLIRELKEDDIKNALDLIWNVFEEFESPDYCDEGITTFHQ
                     FIEYNSIIEKIKEKTLFFWGCFDSKKLIGVLAIRNLNHICMLFVDKNYHKHGVAKKLM
                     TTGIQYCICNKFNLTEITVNSSPYAVEVYHKLGFEDIAVEQIVDGIRFTPMKLLI"
     gene            complement(933..1490)
                     /locus_tag="KPH29_003498"
     CDS             complement(933..1490)
                     /locus_tag="KPH29_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG1469059.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKATIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSLL
                     SKEEAIKGLADSIDTVLLGIHKKYD"
     gene            1748..2446
                     /locus_tag="KPH29_003499"
     CDS             1748..2446
                     /locus_tag="KPH29_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG1469060.1"
                     /translation="MEKKYFEIHGVPSILWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPHEYQVLSVDLPEHGERKREKNSFNPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNVPTKILYGKHDELTEIGVVEKFVEKFNCNLTILENGKHWFNTPQ
                     ELNSLYEWLNTLVK"
     gene            3468..3935
                     /locus_tag="KPH29_003500"
     CDS             3468..3935
                     /locus_tag="KPH29_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBG1469061.1"
                     /translation="MITKFFSNILSVFLYFLLSLLGVAGIIFLCMIPMFLYDFSSDKV
                     IALIARILYFSDYFIVILFLIFIINSTKYSPFILENVKRFKVMGCCLLINTIIECVIG
                     YNSNTSLIMIFGDNSGGISPLMVISFIFALMCFVIAETFDKAIKIKEDNDLTI"
     gene            3946..4161
                     /locus_tag="KPH29_003501"
     CDS             3946..4161
                     /locus_tag="KPH29_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1469062.1"
                     /translation="MAIVVNLDVMMAKRKISLKDLAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
     gene            complement(4931..5215)
                     /locus_tag="KPH29_003502"
     CDS             complement(4931..5215)
                     /locus_tag="KPH29_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1469063.1"
                     /translation="MLVYNKSFYPNDIFPRLDFSKIKKQLKLIDNDLSDFGRICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
BASE COUNT         2008 a          655 c          579 g         2117 t
ORIGIN      
        1 aaatttataa aacaaattgt attgaattaa atttatttaa gatatataat tataattata
       61 attaaaataa taataaaggt gttgtttata atgttcaaag aatacttaat aagagaatta
      121 aaagaagatg atattaaaaa tgcattagac ttaatctgga atgtatttga agaatttgaa
      181 tctccagatt attgtgatga aggaattacg acatttcatc aatttataga atataattct
      241 attattgaaa aaatcaaaga aaaaacactc ttcttttggg gttgttttga ctctaaaaaa
      301 cttattggag tcttagctat tagaaattta aatcatatat gtatgttatt tgtagataaa
      361 aactatcata aacatggtgt tgcaaaaaaa ttaatgacta caggcataca atactgtatt
      421 tgtaataaat ttaatttaac tgaaataact gtaaattcat caccttatgc tgttgaagtt
      481 tatcataaat taggatttga agatattgct gttgagcaaa ttgttgacgg tatacgtttt
      541 acacctatga aattgttaat ataataaatt aaataaaaag caaagtctct taaatatttt
      601 atagttactt tgcttttaaa ccaagattta aaataatact atcttttttg ttttttaata
      661 atatacttta aaagccttac taatatgaac tataatttat tttttgatat cctttttgat
      721 taacatataa attaattcaa ttattaaaca taaactatgt tgtttctctt tatatctctt
      781 ctttgtaaac aatattgggc atttgaaaat aaatattctt aaaatatact gttctcctgt
      841 tgattttcct ttggtacaaa tgataaaatt ttaatttaca tgctccgtaa tgaagtgttt
      901 aataaataag ttaaaataat ttatttacct ccttaatcat attttttatg aatacctaat
      961 aacacagtat caatagaatc ggctaaacct tttatagctt cttctttact caaaagtgat
     1021 ttcccaagtt gcaaagtcga ataaccatga agtacactca ttattaagtt taatacttca
     1081 cctgtattct ttgtatttag attacaagaa agaataagtt tttctaataa ggatttatat
     1141 tcatcaaaga tttctaatgt ttcattattt ccatgccaag tagcccactg aattgtttca
     1201 taaacaccag gattagaaat tataaaatta aagtatgcta tacttataca ctttatagct
     1261 gtatctccaa aatttccaat agtcgccttt atcatttgac tattcatctc tctcattcct
     1321 ttatgagcta cttcaagtaa taaatcatct agactttcta tatgattgta caaagatggt
     1381 gtacgtattc ctagcttttc tgcaataact tttaaagaaa ctttgttaag cccatcttta
     1441 ttaactatat ctgatgtagc ttgtataact atttctctac taacacgcat gattttccct
     1501 ctttcattta aaattaatta ctttattagt ttagctaatc taattagttt cgtcaattat
     1561 tgtaatttat aaaattcttt atactcttat aaaatcttga cagtttaatt ataatttaat
     1621 ataatactaa ttgaattagt tttaatacta atataattag tattatatta aattatatat
     1681 tacaacatta ttttatagtt tgattaattc aacaatcact aatatatcaa actgaaagga
     1741 gatttttatg gaaaaaaaat atttcgaaat tcatggagtt ccttcaatcc tttggggaaa
     1801 tccttcaagt aaattatata tttacataca tggacaatgc ggcaataaag aggaggctga
     1861 agcttttgct aatattgcag ttccacatga gtatcaagtt ttaagtgttg atttgcctga
     1921 acatggagaa cgtaaaagag aaaaaaattc atttaaccca tggcatgttg ttccagaatt
     1981 agtatctatt atggaatatg caaagtgcca ctggaatcaa atttcgttat atgcaaatag
     2041 cattggagca tggtttagta tgttaagctt tgaaaaagag aacttaagag agtgtatttt
     2101 tgtatctcca attctagata tgcaacagct catttcaaat atgatgcttt gggcaaatgt
     2161 atcagaagaa cagcttaaac atgaactaat tatcccaact tcatttgggc atactctttc
     2221 atgggaatat ctacaatatg taaaacaaca tcctatcact caatggaatg taccaacaaa
     2281 aatattatat ggtaaacatg atgaacttac tgaaatcggt gttgtagaga aatttgtaga
     2341 aaagtttaat tgcaacctta ctattctgga aaatggcaaa cattggttta acacaccaca
     2401 agaattaaat tcattatatg aatggttaaa tactttagtt aaataaaaaa taattatatt
     2461 attaatatga ttaaattatt tataaatgtt atttaattaa ttttatttta gcattattaa
     2521 aataactaca taaagaataa attagttatt attaaagaaa ttaaaagtat atagagtatt
     2581 aataattttt taataattag ttattataga aaaaagaggg atataatatt gtctccctct
     2641 ctttcttctg taatattata taatttttat ttatcactct caataaatta tagttatcta
     2701 caaaattcaa tctttaatac attattaata tattgtgata ttaatattac agtattaaat
     2761 aaaatctaat aaaattaact ataaaatatc tattatataa atatccactc ttatgtttga
     2821 aatctgtcat atagttttat ccttcttatc ttttatatca ataacttttt ttaataaaat
     2881 tgacaaaaca aagtaaaaaa ccttattgat aatgaaacaa atgatactat aagttctgat
     2941 attatagtaa ttgcatataa atagacatca taacaataat ataatattgt cactataaaa
     3001 ttaaatgtaa aaatctccaa tctaactaaa tacacatata ggtatttttt aagtttttag
     3061 gtatttcttc tgaaaatgat ttattgattg ataccaattt acataatctt tttaatataa
     3121 acaatgatac aatatatggg acagcacata tgtatatagc tacttatatt acatattaca
     3181 gttggcatat tcgtatttgt aatgcttata ccaattttaa aatacaccat cagtactaat
     3241 ggcattaaaa taatctataa taaagttaaa attattccaa ttagtacaat attatttaat
     3301 attttacaac tcatattctt ttttatataa atttaccacc ttatacattc ttatttttag
     3361 tgtatattat aatggtttta tatattatat caacaattta ttattgaaat tcaataaatt
     3421 atatattata atcaataata tactttatat aaaggagatg atattttatg attacaaaat
     3481 tttttagtaa tatattatca gtatttctat attttctatt atcactttta ggagttgctg
     3541 gtataatatt cttatgtatg atacctatgt ttttatatga ttttagttct gacaaagtaa
     3601 tcgctttaat tgcaagaata ctatattttt ctgactactt tatagtcatc ttattcttga
     3661 tatttataat taatagtact aagtattccc cattcatact tgaaaatgta aaaaggttta
     3721 aagtaatggg atgttgttta ttgataaaca ctataattga atgtgtaatt ggttataact
     3781 caaatactag tcttataatg atatttggag ataatagtgg cggtatctca ccacttatgg
     3841 ttatttcttt tatatttgca ctaatgtgct ttgtaattgc agaaacattt gataaagcta
     3901 taaaaataaa agaagataat gatttaacga tatagaggtg atgttatggc aatagtagtt
     3961 aacctagatg taatgatggc aaaaaggaaa atatcactta aagatttggc agaaaaaata
     4021 gatattacaa atgcaaattt atcaatttta aaaactggaa aagcaaaagc tattagattt
     4081 accactctaa acgaaatttg caaagcttta gactgtcagc caggtgatat acttgaatat
     4141 gtagaagatg aaaatgatta aaaaagagtg tttagaaaca ctctttttta ttatactata
     4201 tgatttttat tcttttacta tattattgac attaatatta actttgttta aagtcataac
     4261 aaaatacgtt ctaagaaaga aactgttcct ttttctattt gttatccatt acctataaga
     4321 actgtatcac actaaatcat acttttaaat tgataatttt gccacagatt cctatataca
     4381 ttcttttcta cctaatattt atttaaactg ttagcaaaac atggaacttt ttcatttact
     4441 tatacctcca tttatattat tgcatagttt atagctacgt gttataaatt acattattga
     4501 tgatttatct aacatttgta atcttttaga tactctttga agttacaaca ctgttatact
     4561 ctaattaagt aaactaaatc tgatacaagt tggtgattat atgaaaagaa aactttcaac
     4621 ctattttaaa ggatatattt taggtttatg tatagcattt ttattattta cttttatatt
     4681 aaaaaaatta attatagaaa atatcattgg aataataatt ggaactggaa ttgcattttt
     4741 actgatttac ctatttaata aaaaaagaaa ataatattct ataaaaaggt cttttagtga
     4801 atttacaaaa aattaataag aataatatat acttagtatt taaaaagggt aacaactctc
     4861 tctagctgtt accttatata tttatctata tctttagtat taagtttttt aatttctact
     4921 tttttaatta tcaatttctg tatgtagata tactaaacct tccaacttga gatttaaata
     4981 acttttcaat ctcataaact attctataca ccttattttc gtactctaaa tcataataca
     5041 catttatttc acctatactg tttacactta tcgtataatg ttctttttct attatacata
     5101 ttcttccaaa atctgacagg tcattatcta tcaattttaa ctgttttttt atttttgaaa
     5161 aatctaatct tggaaatatg tcattaggat aaaaactttt attatataca agcatttcat
     5221 ccacccctta tttattctat ttatatcatt ttatacccta attaataatt tattaatcat
     5281 ctttttccat attattcaaa ttctcttata taaaaacaaa atcaatttag aatatgtatt
     5341 attaaatata aagtgactc
//
LOCUS       DAELXX010000177         5347 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.179, whole genome shotgun sequence.
ACCESSION   DAELXX010000177 DAELXX010000000
VERSION     DAELXX010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5347
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.179"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(204..1388)
                     /locus_tag="KPH29_003503"
     CDS             complement(204..1388)
                     /locus_tag="KPH29_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG1469064.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMIKLVNAVPVFIDTKKENGFKLTKEELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1761..4112
                     /locus_tag="KPH29_003504"
     CDS             1761..4112
                     /locus_tag="KPH29_003504"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HBG1469065.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERFEVQGNKKMKNFPFLMGQGVWKGSDDLVPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4134..4673
                     /gene="nrdG"
                     /locus_tag="KPH29_003505"
     CDS             4134..4673
                     /gene="nrdG"
                     /locus_tag="KPH29_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBG1469066.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAREAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKKVFLVEQYMKDDLSIAE"
BASE COUNT         2089 a          593 c          850 g         1815 t
ORIGIN      
        1 ttataataaa tttagaattt aacttatata gtttaatttg ataatttgat gaaaaaaata
       61 aaagacttac tgatttttaa atctataggt cttttatttt ttatctaaca tttaaattaa
      121 gttgttgaat atatcttata ttacttactt tatatattaa ttaccattaa tacattatat
      181 ctaattacta ttcatctaaa ttattatgcc attattttat atacaaattc ttttaatttg
      241 tctaatccag ataaaaaagt attttcactg caagcatagg atatacgaat atatttatcc
      301 ataccaaatg ctatgccagg aacaactgca acattatatt cttctaaaaa ttgattacaa
      361 aattctatag aaaaactatc tttatattca aatttctcag atactttaga aagatctata
      421 aatatataga atgcgccatt tggattcaca tatcctacat tatcaataga gtctagtttt
      481 gatttaatta aatctcttct tgatttatat gttttaacca tattgtctat gtcaattgag
      541 caatctttca aggctccata ggctatgtat tgtgctgtta gacttgggtg agatataaga
      601 tgtccttgta tagaactcat tgctttagca atcgttttat tagatgcagt atatcctaat
      661 cttaatccag tcattgcagc tgacttagaa aaaccattta tagttattgt tatatcttta
      721 gcttcttcgc ttaatgatgc aattgatgta aattcaccag tgtagcatat tttttcatat
      781 atttcatctg ctaaaatata tattttattt tgtatgcata catctactat ttctattaat
      841 tcatcttttg tataaacact accagtagga tttgaaggat tgtttattac aagaattttt
      901 gttttatctg ttatagattt ttcaagttct tcttttgtta acttaaatcc attttctttt
      961 ttagtatcaa taaaaactgg aacagcatta actaacttaa tcatttcagg ataactaacc
     1021 caataaggct ttggtaataa gacttcatct ccttcgtctg ttaaagctag taaagtattt
     1081 gttattgagt tttttgctcc acttgataca actatttcgt ctattgaata gttacagtta
     1141 ttttcttcaa taagtttttt acagatttcc tctctaagta tttttaatcc tggaactaaa
     1201 tcatatttag tgtaatcttt atttaaagaa tcaataccat aagattttgc gttattaggt
     1261 acattaaaat cgggttctcc tatactaaga tttattactt taactccatt actttccatt
     1321 tctttaactt tagaactaat acctattgtg taggatggtg ttataaagtt tagtcttttt
     1381 gataacataa aatgcctcct tttaaattat tatttatatt atacgacaaa tttaaagaat
     1441 ttacataagg aaatttgtag atatatacat taatatatat aatagcctac actttttgcc
     1501 taaaattagt gtaaaaaaaa gagaaaaaaa ttttaacttt caaagttgtt agaaacgcta
     1561 ctgttcaaaa tatccacata gtatttgcaa tgatttgcgc aagtttgact attatgtaca
     1621 aatttggtaa tcttaatata gcaattatta agataatccc tttaacttag ttgaagggga
     1681 tttttttaga cttaacatac tacatatagt ggtattggga ttaataaaat acaatatata
     1741 gtaacaggag gtttttttaa ttggttgaat tcgtaaaaaa aagagatgga agagtaattc
     1801 catttaatga agatagaata acaagagcta tatttttagc agcaacgaat gttgcagaaa
     1861 gagaaggaat agtacctgat tataaattat ctgagcaatt aactcaagaa gtaataaagt
     1921 ttttaaatca taaatactca gaatcagttc caagtgttga agatatacaa gattctgtag
     1981 ttaaggttct tatagaaact ggccatgcaa aaactagtga agaatatata atatacagaa
     2041 ctgaaagaag tagaataaga aattcaaaaa caagattgat gaaggcaata gaggaaatta
     2101 cctttgaaga tgcagaagat gctgatataa aaagagaaaa tgcaaatatt aatggtaata
     2161 cagcaatggg aactatgtta caatatggaa gcactgtatc taaagaattt tgtaaaacac
     2221 atatattaaa accagaacat tcttttgctc atgataatgg agatatacac atacatgata
     2281 tggatttttt aaatatggga acattaactt gttgtcaaat agatgttaaa aaactattca
     2341 atggtggatt ttcaacagga catggctttt taagagagcc acaagatata ataagttatg
     2401 gtgcattagc tgcaattgct atacaaagca atcaaaacga tcaacatgga ggtcaaagta
     2461 taccattttt cgattatgga ttggctgaag gtgtatataa gacatttaag aaattctaca
     2521 taggaaattt agctaaagca ttgaagttat ttaaaggaat tgaaaataat gatgtgataa
     2581 agaacatagt atataataca gagaaagaaa caaatcaaaa agttggtctt aagagagatg
     2641 aattgtatct aaacttagaa aaagagaaat taatacagac ttttgatata gatgatgaac
     2701 ttgtaaataa gatgcaaaac tttgcatttg aagaatcgta tagagaaact gataaaaaaa
     2761 catatcaatc tatggaagca tttatacaca atctaaatac aatgcactct agagcaggtg
     2821 cacaggtacc attttcaagt gtaaacttcg gaacagatac ttctgaagaa ggaagaatgg
     2881 taactaagaa tcttctatta tctcaagaaa gaggtttagg taatggagag acaccaattt
     2941 ttccaatttt aatatttaag gtcaaagaag gcataaattt aaatcctgaa gaccctaatt
     3001 atgatttatt taaattatca tgcagagtat cagctaaaag actatttcct aattttagtt
     3061 ttttagatgc gccttttaat gcaaaatatt ataaaaaagg tgaaccagat acagaggcta
     3121 catatatggg ttgtagaaca agagtactta gtaatgtgtg tggttcagaa acagttagtg
     3181 gtagaggaaa catatctttt actacagtaa atttaccaag attgggtata aaacatggta
     3241 ttataaataa tgaaaaggct aatttagatg gtttctttga agaattagat gagaagataa
     3301 acttaattat agaacaatta ttggagcgct ttgaggttca aggaaataaa aagatgaaga
     3361 attttccatt cttaatgggg caaggtgtat ggaaaggttc tgatgattta gtaccagaag
     3421 atacactaaa agaagtaata aaacaaggaa cattgacaat tggatttata ggtttagcag
     3481 agtgccttat tgctcttata ggtaaacatc atggagaaag taaagaagct caagaattag
     3541 gattaaaaat agtgtcacat atgagacata aaatggatga agcaacggac aaatataaat
     3601 taaatttctc cttaatgggt actccagcag aaggtctttc aggtaggttt actaaaatag
     3661 ataaaaaagt ttatggagaa attaaaggta tcacagataa agaatattat actaattcat
     3721 tccatgtacc agtttattac aatataagtg catatgataa aattgagata gaagctccat
     3781 accatgaatt gactaatgca ggtcatataa catatgtaga gttagatgga gatccatctg
     3841 ataatttaga ggcttttgaa actgttataa aagctatgaa agatttgggt ataggatatg
     3901 gaagtataaa tcatcctgta gatagagacc caatatgcgg tttttcaggt gtaataacaa
     3961 gtaatatatg ccctgtatgt ggaagaaatg aagatgaaag tgacatcaaa ttcgaaagaa
     4021 taagaagaat aacaggatac ttagttggta cagttgatag atttaataat gctaaaaaag
     4081 cagaagtaag agatagagta aaacatagat agttttattt gggggaataa gttatgaaaa
     4141 taagaatgtc atctactata agttatgaca gcatagttga tggaccagga ttaagaatgg
     4201 taatatggac tcaaggatgt attcataatt gtaaagagtg ccataatcct cagacacatg
     4261 atttatgtgg aggattttat atggatacag aagaaattat aaataaagtc aaatcattga
     4321 aattacaaaa aggcattacg ctatcaggag gagaaccatt tttacaacca gaaccattag
     4381 aagaaatagc aagagaagct aaaataaatg ggttagatgt atggtcatat actggattta
     4441 cgtttgaaca actgttagat aaaaaaaata gagcgtattt taaaaattta aatttactaa
     4501 agcaaattga tattttagtg gatggaaaat ttatagctga aaaaaaagat ataagcttaa
     4561 agtttagagg gtcatcaaat caaaggataa ttgatgttca aaaaagtttg aaatataaaa
     4621 aagtgttttt ggttgagcaa tatatgaagg atgatttatc tatagcagaa taaaatataa
     4681 ataaacagga ttaaatatat gcatatattt aatcctgttt attttcatat aatatttcaa
     4741 gtttgtataa tgtaacaatt aaaccaaaag actcattgta tatttgaaat taagtttttt
     4801 atagtttata ggtgttttat gtggattacg aatgatatag tatcgataca ttatttaatc
     4861 gattcttatt ccaagaaaca attaatttat agaaattctt aattgttaca atataaatat
     4921 ctagataagt tataagatac tgataaataa tttataaagt ttattaatct aatattgtgt
     4981 gtgaatgtta agtggtcaag tagtctaaaa aatgaaaaag atttaaaaaa gtgttttaac
     5041 taaacacata agtgttaaat tcatgtataa gtataggtta gatacataag aaataattat
     5101 gtaatatcta tctttgtaaa aaaatattaa attttgtaat ttgtggataa atatgtttat
     5161 aatgtggata atatttaatg taataagtta aagcaatatg aatcaactgt ttataatgtg
     5221 gataatatta taaatgtatg aataattgca gtatttaaaa acaaaaaaag ttaaaaaagt
     5281 tgttgactct aaaagaaaaa gttggtatag taatacttgt cctcaagaaa agggcaaaca
     5341 aataaag
//
LOCUS       DAELXX010000178         5262 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.180, whole genome shotgun sequence.
ACCESSION   DAELXX010000178 DAELXX010000000
VERSION     DAELXX010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5262
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.180"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            546..1853
                     /locus_tag="KPH29_003506"
     CDS             546..1853
                     /locus_tag="KPH29_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1469067.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLVLDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYHNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQSKILTDEAFDCNLASKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    1939..2201
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2327..3130
                     /locus_tag="KPH29_003507"
     CDS             2327..3130
                     /locus_tag="KPH29_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG1469068.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINKTLKEMKKDGTFDKIYDKWFK"
     gene            3168..3779
                     /locus_tag="KPH29_003508"
     CDS             3168..3779
                     /locus_tag="KPH29_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG1469069.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            3811..4482
                     /locus_tag="KPH29_003509"
     CDS             3811..4482
                     /locus_tag="KPH29_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBG1469070.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
     gene            4781..5143
                     /locus_tag="KPH29_003510"
     CDS             4781..5143
                     /locus_tag="KPH29_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBG1469071.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
BASE COUNT         2134 a          459 c          927 g         1742 t
ORIGIN      
        1 taaattaatt atgaaaaatt tatataagta aaatatatta acataaaggt aaaattttgt
       61 atactaaaag ggaatattta cagtttaatg gcagttttaa aataataaat taaagctgtc
      121 atttttattt aaaaattttt agaaaaagta caatagtgtt aatgtgtgaa ttagataagg
      181 tgtatataat aatttagatg atatgatata aaatatgttg taaattaaat gttttctttg
      241 acaatatata cacaaggatg ataatatcta tatgaataaa agatagtgtg taatttgata
      301 atatgtaatt ataacattat gatataatta caataaagta tgtaataatc tgtagaaatt
      361 tcaagctagt tatctaagtt ttcggataac taaatctaaa cggaatacat aatgtattaa
      421 ataaatgatg atactaaaat gatactattt ttatagaaat ttattgaggt atataattga
      481 tgaaaataat atattaaatt atataatata ttgagtttta cataaaagcg aaggtgggaa
      541 taaaaatgaa agaaattgct gatgctataa catcagagta tttaagctat ggtatcaatg
      601 aagatattag tatctatgaa ggtagatttt gcatatattt agataaaaaa tatagatgta
      661 atgggaaaat ttattataaa atgacacctc caatatctat aagttttaaa gcagacatag
      721 gttgtgttga agagatagac aatgaagatg acaatttagt tttggattat gataatgcta
      781 ttcttgaagt tcatggatac aaaattattt ctataacaat aaatacttta agtgaattta
      841 gtgtagaagg ttatatcaat gatgactgca taaaatctaa gaactcctat gttgaatatg
      901 tagattttaa tataataaac ttagataaaa tacctggaaa actaattaaa tacaatgata
      961 aggtatacgc aggtagaatt gaatttgata taaatgatta tgttgtaact atagacaagc
     1021 gatatgatta tagaaaagaa ttaaaatcag aattaaagtc taaatctgga gcaataataa
     1081 ctcatatagg taggattaga agaaaagatg gtagaatttt tagaacaaat aatacaataa
     1141 atcttttaga tagaatatca acagctttga gttttatgtg tggaagatat gtagggtttt
     1201 gtttggccaa agggtataga agtggtaatg aagtttatag aatatggaat gaaaatcaaa
     1261 tatctccttt tagatatgta ccaacatggt cagatacact ttcaaattat cataatatgg
     1321 aaaaatatat aagccttatg tgtaaaaagc tagaagattt ttattatggt tctgctataa
     1381 agagtgttgt tgattggtac atagaatctc ttggaagtgc taccatggaa aacaatataa
     1441 tatctgtaca aattgcactt gaaactttgt catatgtaat tctagtggaa caaagtaaga
     1501 tacttacaga tgaagcattt gactgtaatt tggcatctaa aaatataaga ctcttacttg
     1561 atacatgtaa gataccatat ggtaaacatg aattaaatat atttgataat ataataaaaa
     1621 ataaatttga tgatggagtg gatttagtta tttatttaag aaatagcata gttcatccaa
     1681 gtagaaagac tcatagagct gtgttagagg ttgaagatat ttggaatata atttcaatag
     1741 gaacgagata tattgaactt gttttactct ttattttagg ttatagagga gaatattcaa
     1801 acagattagt agaaaggtgt tatggtgaag tggaagttgt accttggaac tagattttta
     1861 tatttatttt aaaatatata ttgacatata ttgccttatg ctataaaatc aatatcataa
     1921 taataattca gaataacatt aaatgctaag aaaagaaata gtaaaggtag ttaatcttaa
     1981 acagagagtt tccgattggt gagaggagca atagatttta ctttgaacac atctttgagc
     2041 aggactctga aagagtagaa atactttatt aggagatttc cggaatcctt gcacccgtta
     2101 tagtgctaga gtataataat tttattgtac ttaaagaggt tagtgtcgtg aggcattaat
     2161 gaacagaggt ggaaccacga gagttaactc ccgtcctcta gcagaagcta gaggcgggag
     2221 ttttttaata taatttttta agcgcgcaga gaattatatt gcaatagata cactttttaa
     2281 aaaataatca aaatatttta aaaattaaaa ggcgagggag aatattatga aaaatatatt
     2341 aaaaaaagta ggaatattta caattatgtt aggtttatta ggtggagttg taggttgttc
     2401 aaaaccagat aatgaaaaag ataaggatgc ttcaaaggaa tctaaaaaag aagtagttgt
     2461 agggtttgat aatacgtttg taccaatggg atttttagat gaaaagggag atacagtagg
     2521 atttgacgta gacttagcta aagaaacatt taaaagactt ggaatggaag ttaagtttca
     2581 gcctatagat tggtcaatga aggaaactga attaaatgat tcaaaaactg tagatgtatt
     2641 atggaatgga tattctataa cagacgaaag aaagaaaata gtatcatata cagaaccata
     2701 tttacaaaat aaacagataa tagttactct aagtgattca aaaataaatt caaaagctga
     2761 tttaaaagat aaagaagttg gaacacaaca aggttcaaca gcacttgatg cagtggaaaa
     2821 ggataaagat tttatgaata gcttaaaagg tggagcacca gttctttatg atacttatga
     2881 taaagcactt agagatttag agataggtag aactagtgca gttgttggtg atgaagtttt
     2941 aattagatat tatatgggac aaaagggtga agacaaatac aaggtattaa aagacgattt
     3001 tggattagaa gattatgttg tagcaacttc taaagaaaat ccagaacttt gtgagaaaat
     3061 aaataaaaca ttaaaagaaa tgaaaaaaga tggaactttt gacaaaattt atgataagtg
     3121 gtttaaatag aagataagtt agctgttaag aagggggcac taaaggcatg ttacaaggtt
     3181 tagagatagt tatagcaatg ttttgtataa cattgatagt atcaattcca ttggggattg
     3241 gagttgcatt tttaagatta tcaaagaata aattagtaag tggtattaca caatgttata
     3301 tattgataat gagaggtaca cctttacttt tgcaaatgat agtcatattt tacggattac
     3361 ctcttttagg aattgtattt gacagattta cagctggagt agtagctttc ttcttaaatt
     3421 atgcagcata ttttgcagaa atatttagag gtggaataca gtcaatcgat agaggacaat
     3481 atgaagcttc aaaagtacta ggatttgata aatttactat gtataagaga gtaatctttc
     3541 cacaagtttt taaaagaata ctagcaccta tttcaaatga agtaatcaca ttagtaaaag
     3601 atacatcttt agtatatata ttaggattaa atgatatttt aagaatatct caaatagcaa
     3661 tgaacaggga agcaagtctg ttaccattat ttgaggcagg agcgatatat cttatatttg
     3721 tagctatact tactaaggga tttgaattac ttgaaaaaaa atattcttat tatagataaa
     3781 gttacaatat tataggagga ggttttttat atgttgaaaa tcaaaaactt aaataaatca
     3841 tttaaaaaaa atagagtact taaagatatt tcttttgagt tagaagaagg tcagattgga
     3901 gttttacttg gtaagtctgg tgcaggaaag accacaatat taagatgtat aaatggtctt
     3961 gaagaatttg atagtggaga gattataata gataatgagg taataaaaaa taagagagat
     4021 atggcaaaaa tacgaggaaa aataggtatg gtttttcaaa atttcaactt atttcctcat
     4081 atgacagtcc ttgaaaatat aattgaatcc ccagttaatg tttttaaagt tcctagaaaa
     4141 gaggctgaag agagggctag agaactttta agattagttg atttagaaga taaattaaat
     4201 tcttatccat ttgaattgtc aggaggacag caacagagag ttgctatagc taggtcatgt
     4261 gcattgatgc caaaagtcct ttgttttgat gaacctactt ctgcacttga tattgatact
     4321 attcaaagag tagtcaatat aatgaataga cttaaagaca aaggtatgac aatattaata
     4381 attacacatg atgttgtctt ctcaaataat gttgcagata aaattataag tataaaagat
     4441 ggtatagtag aaaatgttca aataaaagaa aaaattgttt agagttttat aaatataaac
     4501 tagggtagaa ttttataata taaataaagg gcaagtaaaa tcaaatatac tatgcccttt
     4561 tgttatctca aaatttaatc ttatatttta tgtttaagtt acattataat ttataataaa
     4621 aataaaagga ctatttaaat atataatgtg aataaatgtt taataatttg aaatatgata
     4681 attttttatg ttgaaagctt ataatgatta tgatataaat tggttatagt atacaattta
     4741 tgggtataac aataaaaaat tagaaaggaa tttttataaa atgattaaat tatatggata
     4801 tacaaaatgc tctactgtta aaaaagctaa aaattggctt aaggaaaata atttagaatt
     4861 tgaagatata gatatggttc aaaaccctcc tagtaaagag gagttaaaat caatttataa
     4921 gactagtgga tatgatataa agaaattttt taatacaagt ggtatgaaat atagagaatt
     4981 gggtcttaaa gatattgtaa aaactgaatc tgatgataaa cttttagaaa tattggtaag
     5041 tgatggtatg cttattaaaa gaccattact tttggatggt aaaaatgtat tattgggctt
     5101 taaagaagat gtatggaaaa gtactttgct aaaagaagat taggaagaga taggtgtgtt
     5161 gattaaaatt attaattaat acacctataa atttatgtat taaatctaat ctttataaat
     5221 attaattagc aaattcaaat caaaaaaatt catatcaaca aa
//
LOCUS       DAELXX010000179         5223 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.181, whole genome shotgun sequence.
ACCESSION   DAELXX010000179 DAELXX010000000
VERSION     DAELXX010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5223
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.181"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            606..5117
                     /locus_tag="KPH29_003511"
     CDS             606..5117
                     /locus_tag="KPH29_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HBG1469072.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANIPVVLQDIATGVRLVVL
                     TDASGNYVFTNVPNGNYRIVEAFGTTGGVPTPGNFATAIVGPVPIAEMPPISSVTNPP
                     IGSTNLDGTTPNTLLITVAGSDIINQNILNGPVIYTPIENILDSCVSVSNINLITDAD
                     NGTFGFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSN
                     VIGAWWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQVTPNTNYLFSTWILNLFKVT
                     GYPPPQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEVGTVINSQNNNQLTVEFFS
                     EGESAIGNDYAIDDVSLREIAVPTFTPIKTSNTETANVGDIITYTVTLDNTCDSPLTN
                     VFFRDLIPNGLEFIPGTVTVDGVSVPGVDPNTGFPLPDIGGGIGVEVTFDVVVESIPN
                     PNPTNNIATIDYSYTPVEGGIPNDFSVDSNPVPVEVISADVEVTKLSEPTIVNPGEEL
                     IYTIKVVNNGPFPSENVVLTDDVPASIINPEYSLDGGVTFQPWTGSLNIGTLEVGETR
                     VIIIRGIVNPSTVGIITNTAVVSSTTADPNLNNNTSTIETEVNLLADILVMKTAEPNS
                     AVPGTLLRYTIQVENLGPANAENVILNDDIPASIINPEYSLDGGASFQPWNGSLNIGT
                     LNSGISLTVLIQGTVSLNSSEYIVNTATVSSTTPDPDLSNNISTIITPVNPQAGISII
                     KVADEDVAVPGEEFVYTIEIFNEGPSNATNVVLTDDIPDVILNPEYSLDGGATFQPWN
                     GSLNIGTVAPGQLIRIIIKGLVSSTATGDITNTAEVSADVPEPVTDSSTVTTPIVPSA
                     DIEVIKTSNMDTAVPGETFSYTITVINLGPSAAQSIVLTDDIPDVILNPEYSLDGGVT
                     FQPWNGNLSIGTLDAGEIRSIIIRGTVSQTAVGTIINTATISSPTPDPNPDNNTSTDE
                     TDISPLADISVIKGNEPVAIPGGRFIYGIEIANAGPSFAENVTLTDNIPASILNPEYS
                     IDNGVTFQPWNGSLNIGTLDAGEIRNIIIRGTVSQTAIGTIINTATVSSTTPDPNLNN
                     NTSTSEAEVSSSADISVVKRSNQTVVVPGDVLDYTIEVRNAGPSTAQNVTLTDNIPAS
                     ILSPEYSIDNGVTFQPWNGSLSIGTLDAGEIRNIIIRGIVSQTAIGTIINTATVSSTT
                     PDPNLNNNTSTSEAEVSSSADISVVKRSNQTVVVPGDVLDYTIEVINAGPSTAQNVTL
                     TDNIPASILSPEYSIDNGVTFQPWNGSLSIGTLGAGEIRNIIIRGIVSQTAIGTIINT
                     ATVSSTTPDPNLNNNTSTSEIDISSSADISVIKLANKTEACVEEQIDFVIVVSNAGPE
                     NAQNVTLIDNVSDKLKKAVFSLDRGVSFQPWTGSLNIGTLPAGTLRVILLRGIIKSTC
                     LDRLTNIAEVTSTTPDSNLNNNISRVQVEIKQCCCNDCCCNNCSNCCCNDCGCKDCCC
                     NCCKNDFWC"
BASE COUNT         2008 a          779 c          895 g         1541 t
ORIGIN      
        1 attaaagtgc cagattaagt atttaagtag attttaagct tgatttgatt agaaaggtac
       61 aaactatcac cgaaaaaaat taaacccatt taaaaagcaa tataaaagct tgttattttc
      121 gagaaaaata gaccctctaa actcaattag ataaaaataa aatacaaaaa attatatctc
      181 tgaatatctc aagtaattaa gtttaaattt tacgatattt tgtttgatga tttataatat
      241 aaaagcatat tcttaatctt ctcaaattaa aaagagtaaa aaataacaaa aaaatcactg
      301 acatttcact gacaaattgt aattttgtta ttataaatac attgaaaata cttgattatt
      361 gaaaaatggc ataaaataat accttttatg ccatttacta ttgtttgata gtttttaact
      421 tatgacataa gatatttttt aacatataca ataaaatata aaaaaattga tattgcaaat
      481 aaaaaaaata catattagat gttatagaaa aaaagcgcac ttataggctt aaaatgtcac
      541 ataaaagagt ttcaaagaat aatatattat gaacacaaat tttgtaatca caaaggagtg
      601 ataatatggc aacagtatca ggacaaatag tttttgatag aaatagaagt gctaccattg
      661 attctgggga ctcaggaata gccaatatac cagttgtatt acaagatata gcaactggag
      721 ttagacttgt ggttctcaca gatgcatcag gaaattatgt ttttactaat gttccaaatg
      781 gtaactatag aattgtagaa gcttttggaa ctacaggagg agtaccaaca ccagggaatt
      841 ttgcaacagc gatagtaggt ccagttccaa ttgcagaaat gccacctatt agttctgtga
      901 caaatcctcc tattggttca acaaacttag atggtacaac acctaacaca ctattaataa
      961 cagtagcagg ttcagatata ataaatcaaa atatattaaa tgggcctgtg atttatacac
     1021 ctatagagaa tatattggat tcttgcgtgt ctgtatctaa tataaacctt attacagatg
     1081 cagataacgg aacattcggt ttttttccac caggtacacc aagtaataca ggacctgaaa
     1141 tagaacctta cccaaatgtg acgccagatt ttacgtatgt tgtaccagac ccaactaagt
     1201 ttacaccttt agatggagaa tatacagtac aaaatattat gacaaatgca atgagtaatg
     1261 ttataggagc ttggtggcgt attgctgacc atactacagg aaatgaaaca ggaagaatga
     1321 tggtggtaaa tggttttaat cctggagctg tattttttaa atcaacagta caagttacac
     1381 ctaacactaa ttatttattt agcacttgga ttttaaacct ttttaaggta acaggatatc
     1441 caccaccaca gctaggtgta agaatatttg accaaaataa taatatacta tatcaagcaa
     1501 cattaggagc tcaaattcca gttaatacaa acgcaccaga atggaaagaa gtaggtactg
     1561 tgattaattc tcaaaataat aatcaactta cagtagaatt ttttagtgaa ggagaaagcg
     1621 ccattggaaa tgactatgct atagatgatg tttctttaag agaaatagct gttccaacct
     1681 ttactccaat aaaaacaagt aacacagaaa ctgctaatgt aggtgatata atcacatata
     1741 cagttacatt agataatact tgtgatagcc ctttaactaa tgtatttttt agggatttaa
     1801 ttccaaatgg tttagagttt atacctggaa ctgtaacggt tgatggagta tctgtaccag
     1861 gagtagaccc taatacaggt tttccacttc cagatattgg aggaggaata ggggttgaag
     1921 taacttttga tgttgtggtt gaaagtattc ctaacccaaa tcctactaat aatatagcta
     1981 ctatagatta ctcctataca cctgtagaag gcggtatacc aaatgatttt tcagtagatt
     2041 ctaatccagt tcctgttgaa gtaatctcag cagatgttga agttacaaaa ttatctgaac
     2101 ctactatagt gaatccagga gaggaattaa tatacactat aaaagtagta aataatggac
     2161 cattcccatc tgagaatgtg gttttaacag atgatgtacc agcttctatt ataaatccag
     2221 agtattcatt agatggagga gtaacttttc aaccatggac tggaagttta aatataggga
     2281 cattagaagt aggagagaca agagttatta taattagggg tattgtaaat ccatcaacag
     2341 taggaattat aacaaataca gctgtagtat cttcaactac agcagaccca aaccttaata
     2401 ataatacatc tacaatagag acagaagtga atttattagc agatatttta gtaatgaaaa
     2461 cagcagaacc taattcagct gttccaggta ctttattgag atatactatt caagtagaaa
     2521 acttaggacc agcaaatgct gaaaatgtaa tattaaatga tgatattcca gcctctatta
     2581 taaatccaga atattcattg gatggaggag caagttttca accatggaat ggaagtctta
     2641 atataggaac tttaaactct ggaatttcat taacagtttt aattcaagga acagttagtt
     2701 taaattcttc agaatatata gtaaatacag caacagtttc ttcaactaca cctgacccag
     2761 atttaagcaa caatatatct accattatta caccagttaa cccacaagca ggtatttcta
     2821 ttataaaagt agctgacgag gatgtagctg ttccaggaga agaatttgta tatacaattg
     2881 aaatttttaa tgaaggacca tctaatgcta cgaatgtagt tttaacagat gatataccag
     2941 atgttatttt aaatccagaa tattcattgg atggaggagc aactttccaa ccatggaatg
     3001 gaagtcttaa tataggtaca gttgcaccag ggcaattaat aagaattatc ataaagggat
     3061 tagtaagttc aactgctaca ggagatatta ctaatacagc agaagtaagt gcagatgtac
     3121 cagaaccagt tacggattca tcaacagtga caactcctat agttccatca gcagatatag
     3181 aagtaataaa aactagcaat atggatacag cagttccagg agaaactttt agttatacta
     3241 taacagttat aaatttagga ccatctgctg cacaaagtat cgttttgaca gatgatatac
     3301 cagatgttat tttaaatcca gagtattcat tggatggagg agtaactttc caaccatgga
     3361 atggaaatct tagtataggc actttggatg ctggagaaat aagaagtatt ataataagag
     3421 gaacagtgag ccagacagca gtaggtacaa ttataaatac agcaacaata agttcaccta
     3481 cacctgaccc aaatccagat aataatactt caacagatga aacagatata agtccattag
     3541 cagatatttc tgtgataaaa ggcaacgaac cagttgctat accaggggga agatttattt
     3601 atggaattga aattgcaaat gcaggaccat cttttgcaga gaacgtaact ttaacagata
     3661 atataccagc ttctatttta aatccagagt actcaataga taatggagtg actttccaac
     3721 catggaatgg aagccttaat ataggtactt tagatgctgg agagataaga aatatcataa
     3781 taaggggaac agtaagtcaa acagcaatag gtacaattat aaatacagca acagtaagtt
     3841 caactacacc tgacccaaat ctaaataaca atacttcaac atcagaagca gaggtaagtt
     3901 catcagcaga tatttctgta gtaaaacgaa gcaatcagac tgttgttgta ccaggagatg
     3961 tactagacta tacaattgag gtgagaaatg caggaccgtc tactgctcag aacgtaactt
     4021 taacagataa tataccagct tctattttaa gtccagaata ttcaatagat aatggagtga
     4081 cttttcaacc atggaatgga agtcttagta taggcacttt agatgctgga gagataagaa
     4141 atattataat aaggggaata gtaagtcaaa cagcaatagg tacaattata aatacagcaa
     4201 cagtaagttc aactacacct gacccaaatc taaataacaa tacttcaaca tcagaagcag
     4261 aggtaagttc atcagcagat atttctgtag taaaacgaag caatcagact gttgtcgtac
     4321 caggcgatgt actagactat acaattgagg tgataaatgc aggaccgtct actgctcaga
     4381 acgtaacttt aacagataat ataccagctt ctattttaag tccagaatat tcaatagata
     4441 atggagtgac ttttcaacca tggaatggaa gtcttagtat aggcacttta ggtgctggag
     4501 agataagaaa tatcataata aggggaatag taagtcaaac agcaataggt acaattataa
     4561 atacagcaac agtaagttca actacacctg acccaaatct aaataacaat acttcaacat
     4621 cagaaataga tataagttca tcagcagata tttctgtaat aaaattagca aataaaacag
     4681 aagcatgtgt agaagagcaa atagattttg taatagtagt atcaaatgca ggaccagaaa
     4741 atgctcaaaa tgttacttta atagacaatg tttcagataa gcttaagaaa gcagtatttt
     4801 cacttgatag aggtgtaagt ttccaaccat ggactggaag tcttaatata ggtacactac
     4861 cagctggaac tttaagagtt attctattaa gaggaattat aaaatcaaca tgtcttgata
     4921 gattaacaaa tattgcagaa gttacttcaa caacaccaga ttcaaacctt aacaataata
     4981 tatcaagagt gcaagtagaa ataaaacaat gttgttgtaa tgattgctgt tgtaacaact
     5041 gtagtaattg ttgttgcaat gattgtggtt gtaaagattg ttgctgtaat tgttgtaaaa
     5101 atgacttttg gtgttgattt tataaaataa ttttgtatac cttaaattaa actgaaataa
     5161 aaaataaatt cacatatgag taaaatattt tataaatatt aataaatttt ctatctttga
     5221 tat
//
LOCUS       DAELXX010000180         4914 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.182, whole genome shotgun sequence.
ACCESSION   DAELXX010000180 DAELXX010000000
VERSION     DAELXX010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4914
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.182"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(545..1186)
                     /locus_tag="KPH29_003512"
     CDS             complement(545..1186)
                     /locus_tag="KPH29_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HBG1469073.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
     gene            complement(1429..2232)
                     /locus_tag="KPH29_003513"
     CDS             complement(1429..2232)
                     /locus_tag="KPH29_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG1469074.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKDKNEVIKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGIEMARDIHQSMKDLIASFAQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYNPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            complement(2388..3563)
                     /gene="thiI"
                     /locus_tag="KPH29_003514"
     CDS             complement(2388..3563)
                     /gene="thiI"
                     /locus_tag="KPH29_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBG1469075.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEEATILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSIVPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            complement(3609..4760)
                     /locus_tag="KPH29_003515"
     CDS             complement(3609..4760)
                     /locus_tag="KPH29_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBG1469076.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRPSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDVVISKVNNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
BASE COUNT         1527 a          929 c          466 g         1992 t
ORIGIN      
        1 ttgaaaattt agatactttt ctaaaaatta tctatgttaa tagttaaatt tatgaacatt
       61 ttttgttttt atgttaggat atttcttaga aatgaaatac ttcttaattt gatttgatat
      121 gtatattttt atagctattt tattataaaa aaaaagaacc cttttacaac taagggttac
      181 tttttttctc tatttttttt attatatcac tattttttcc ctatttcaat attttgacat
      241 tttttccatt atactgaaaa gatatcttaa atttttaact tggataaatt ttaaagcata
      301 tacttaaaat taacatactt gttttctatt ttattacact taatccttat ttttatcatt
      361 ctacaaaaca aaaataaaaa agaataagat aataaataaa atacttatca ccttattctt
      421 tatttaatta ttttacaatg aaaaatctaa actacttttt aaatagcttc ctcttccatc
      481 aatttatttg tatattactg gtttaattct tcaagtaact gattggttct tgcaatactt
      541 tatcctaaac tctggaaact ttgtaaacta cctctctagc atttggtaaa tttccatctg
      601 gaatacctac agcaatacca aatggtacag aatacccttc tggtatatca agtagttctc
      661 tgtatttttt atttgtatca aacttcatct ttattgactc tgattccttt acatcttctt
      721 tatccaatac actcacaaga ccttcaatat ccccttgttc aacaggtgta aataaacctc
      781 taaccattcc tattatacaa cttccaagcc ctagtgcttg tgcagctgtt gttatattcc
      841 ctgtaagtat tcctatatca tatggagccc atgaactact ttcatcataa gaaataaata
      901 aaacggttgg agcaccataa aataacttaa agtttttatt tatctcaaca ttactcactt
      961 catgaatcct agataaagta tcatcactaa tctcttttaa aagtttctgg tcttgcacaa
     1021 cagttatgtg ccaaggctgt ttattacatc ctgaaggtgc tgcaaatgca cagtgtaata
     1081 atgtgtttac ttgctcatca ctaatttgct ctgttgtaaa ttttcgtata cttctacgac
     1141 tttgtattaa atttattgtt tggttgtctt gaaaattatt attcatatct attccccttt
     1201 ttatgtattt cttagtacaa atactaaatc ttcagaaaat taatcttata attaaatttc
     1261 tttacaatat tattataatt atctttagct tgtatgtgaa gacttaaaaa tataaatact
     1321 tttaatatat cttaattata catataaatg aaaatctggt attcgtcatc tatagaaact
     1381 gctcttttct atagtttaat caataccaga ttaataaaac atgatatatt aatttaaagg
     1441 accccatccc tctttagaat gaatctctgt ttttacagac ttaacactat ctgtgtcaac
     1501 atcccatgac ctacctgcaa cagaaatacc atttttacct ttataccatc taccttcatc
     1561 ataatcgtaa actagggaag tataccaatt acctccagtc ttattatttg caaatacaac
     1621 tacagtagca tcagatttag taggattata tgcatatggt tgtatatata gtgtatcctt
     1681 acttaatcca tattttgcct taaattcatc tggaattgta ggccacccat ttttatattt
     1741 attgtctaat aaatattttc taaaatcatc attgcccttt gaagctcctg gtattataga
     1801 agggtcttgt gcaaatgatg caatcaaatc tttcatactt tgatgtatat ccctagccat
     1861 ttcaatacca tctggatgtt ttgtacaagt aacatacact tttgcaatgc tttctattcc
     1921 agttattcca tcataaccat catcaaacgt tgctgaatat attccacctg acttacactt
     1981 tggttctgtt tcaaagtact taccatcttt gttttctaat acctctttta tgacttcatt
     2041 cttgtcttta cttgattcct cagccattgc aataacaatt tgagtcttta tattttctct
     2101 attagaaaga catgtaactg ccttactttt ttctatattt ctaaataacg ctggaacagc
     2161 tactataact aaaattccta ttatagatat aactaccaac aattcaatta atgtaaaacc
     2221 ctttttattc ataataatca ttcccattaa ataaaattat agtacttatt tttaaatact
     2281 atatattaat aaattgtcta cataaatcct ttcaacagaa ctactaatat acttacagaa
     2341 ctactaatat actcaatatt caatttagta attaaataac ttgtatgcta aaactcaata
     2401 tcctctacct ctattttgtc aatagcatcc tgtactaatt tttcaatatc aagtgctgtt
     2461 tcagattttt ctattttctc taatctaggt ttagtgacag gctttctagg agaaaataca
     2521 ctacaacaat cttcttcagg cactatagat gtttcaaaag tccctatttt tttggctata
     2581 tcaactatat ctgatttatc cattgctata agtggtctaa acactggtaa gtctacaacg
     2641 gcattagtac aagtaagccc ttgtatagtt tgagatgcaa cctgcccaat actttcacct
     2701 gtaattaaag catcacaatg tcttttttca gaaagtctct gtgctatcct catcataaat
     2761 cttcttgaaa gaatagtagc ttcttcttca ttacaattct caccaattgc tttttgtatt
     2821 tctaatatgt taactttatg aagtcttact ctaccacagt attttgccag tattttagct
     2881 aaatccttaa ctttttcctg tgacctttca cttgtaaatg gataactatg gaagtgaact
     2941 gcctctacct ccatacctct ttttgcaacc atccatgtag ctactggact atctatacct
     3001 cctgataaaa gagacatagc tctaccattc gttccaagtg gcaatccacc atatccaggt
     3061 atagtatcac tatagaccat agtatgaaat tctctgtatt cgcactttat tttaacttct
     3121 ggatttctta catcaacatt aatcctatct cctacctttg ataataagta tccacctata
     3181 tccatactca tttcttgaga agtcaacccg aaagacttat ctcctcttct tgactctacc
     3241 ttaaaagttt tatatccagc ctctattttt tcttctaaca tttttaaagc tatttctttt
     3301 aatgtatcat aatctttttt agctcttact ccagggcaca cacccactat cccaaatact
     3361 tttctaactt cttcaattac ttcttcataa tcatagtctt ctaaatcaac atatattctt
     3421 ccatactctt tatatacatt aaatcttcca attggtttta acatatttct tatatttctt
     3481 ataagcctat tctcaaatat atatctattc tttcctttta ctccaatttc tccatacttt
     3541 actattaata tattatacac catttcacct cttaaactaa tttattatca ctatctatac
     3601 agtattcttt attttctttt cattattgac cttaagtcac aaattgattc tttcaaaact
     3661 ttcacagctt ctaacatttc ttcttctgta ttcatatcag ataaactaaa tcttattgtt
     3721 ccatttattt cttcatttgt aagacctata gcatttaata cgtaactacc tttcttttta
     3781 gaagaacatg cagagccagt tgatacataa ataccttttt gctctaggta atgaagtaaa
     3841 acttctcctt ttgtccctct aaatgataca tttaatatat gacatactcc atcttctgga
     3901 gaattgattt ttatatcttc tatattatca attatttcat tttttaatat attttttaag
     3961 ttatttactt tcgaaataac aacatctaaa tctttgttta gtattctaac agcttctcca
     4021 agaccatata ttccaggtac attttctgtt cccgacctaa ttccaatttc ctgaccacca
     4081 cctgttaaca taggcttaag gcgattattt tcttttatat acataaatcc aattcccttt
     4141 ggaccatgaa atttatgtgc acttacactc ataaaatcta tattgtattt agatggtcta
     4201 aaattaattt ttccatatga ctgaatagca tcaacatgga aataaatttt ttcttttaaa
     4261 cccttcaaat actttccaac ttcacctatt ggctgtatag ttcctatttc attgttaaca
     4321 tgcatcatgc ttactaagca tgtagttgac tttatcgcat tttttaaatc ctctatattt
     4381 attttcccat cttttccgac ctctaaataa gttacttcaa aaccatcttc ttctaaatct
     4441 tttaatgtat ttaaaacaga aggatgttct atgttagtag atattatgtg attttttctt
     4501 tttttattta aactagtaat acctcttatt atagtattat tacactctgt accacctgat
     4561 gtaaaataaa tctccttttc tttcgctcct agagatttag ctatatcttg tcttacttct
     4621 tttatagctt tttcaacttc tacacctttt ctatggacag aagaaggatt tccatattcc
     4681 gtattaagtg cataaaccat tttatctata acttcttgat aaggttttgt agttgcgcta
     4741 ttgtctaagt atatttccaa cttaattaca cctaccttta tttaaaatct tcaactctaa
     4801 ctattatacc actaattata gtattttttt tgttaatctt tattaaaaag acaatcttaa
     4861 attataaaat attatttcat gaaaagctag ttttttcact ttattacaaa tttt
//
LOCUS       DAELXX010000181         4814 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.183, whole genome shotgun sequence.
ACCESSION   DAELXX010000181 DAELXX010000000
VERSION     DAELXX010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4814
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.183"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..500
                     /locus_tag="KPH29_003516"
     CDS             <1..500
                     /locus_tag="KPH29_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1469077.1"
                     /translation="NFTACYIKESIENSTNIKVCVGYGIGNDIKQAMSSAISANKESK
                     INPLNYSFLVNENYETIGPLLCDKLLTVSNKVTPHINTIAKSSKLSTLTIQKIISVTE
                     ILQRDEVTPKELSSCLNVTVRTANRFLSSLLKSGNATILYEKQNSTKGRPERVYKLFI
                     SENKY"
     gene            complement(628..1818)
                     /locus_tag="KPH29_003517"
     CDS             complement(628..1818)
                     /locus_tag="KPH29_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG1469078.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNLPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSENGGKTLLLRADMDALPIMEETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEESTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGNVPNAIPNKASMSGTLRTFDQFVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEIIGNENVIDFPSAMGSE
                     DFSEILLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(1924..3303)
                     /locus_tag="KPH29_003518"
     CDS             complement(1924..3303)
                     /locus_tag="KPH29_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG1469079.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELSAY
                     GHGCGHNLLCTAHVGAVVGLKREMLENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            complement(3334..4542)
                     /locus_tag="KPH29_003519"
     CDS             complement(3334..4542)
                     /locus_tag="KPH29_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1469080.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLL
                     GFWTNVLPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFRLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIVPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
BASE COUNT         1572 a          928 c          578 g         1736 t
ORIGIN      
        1 aaaattttac tgcatgttat ataaaagaat ctattgaaaa tagtacaaat attaaagtat
       61 gtgttggata tggaattggt aatgatataa agcaagctat gtctagtgcc ataagtgcta
      121 ataaggaatc taaaataaat cctttaaatt acagtttctt agtcaacgaa aattatgaga
      181 caataggacc tttactgtgt gataagttac ttactgtttc aaataaagta actccacata
      241 taaatacaat tgccaaaagt tctaaattgt ctactcttac tattcaaaag attatatctg
      301 ttactgaaat tctacaaaga gatgaagtta ctcctaagga attatcttca tgtttaaatg
      361 taacagttag aactgctaat agatttctaa gtagtctctt aaagtctgga aacgctacta
      421 tattatatga aaaacaaaac tcaacaaaag gtagacctga aagggtatac aaattattta
      481 tatcagaaaa taaatattaa aatccaaaat gcataaaaat attttatcat ctaatattaa
      541 gtaaaattat atatgacttt ctaaaaggta tatctttgca tctttttaag atataccttt
      601 tatgatattt aataatattt tatgttatta tctactaaaa ttattttcta accattttac
      661 tgcacaatga gcaaataaag ctgaaccata aactaatccc tcttcattaa aaattgcttt
      721 aggattatgc ataccaaaat tatatccctg actaatactt cccattccaa tccataaaaa
      781 tactcctgga acccttagta aaatctctga aaaatcttct gaacccattg cagatggaaa
      841 gtctattaca ttctcatttc ctataatttc tttaacataa gatacaatct catctcctac
      901 atccaaatca tttataactg cgggagtccc cattttaaat tccactttag catttcctcc
      961 aaatgattta gctattcctg tagatatttc aataattcta ttttttaatt tttctctaac
     1021 aaattggtca aatgttctta aagttcctga catagatgct ttatttggta tagcatttgg
     1081 tacgtttcct gcattaaaca taccaatagt taaaactaca ggctctttct gaggtttttc
     1141 tcttgaaagt atagtgttta atgctatttg tatatgcgac attatgttta ttgggtccac
     1201 tccaaattca gggcttgctc catgagttgc ttttccttct actgttattt caaatatatc
     1261 cgcagatgcc atagctggac cacgtgaata agctacttga cctgtgttaa gaaatttacc
     1321 caccatactg tgaaatgcca tagctgcatc gacctgagga ttttctaata taccatcatc
     1381 aatcattgat tttgcacctc tggtactctc ttcatctggt tcaaatacaa actttactct
     1441 acctttaagt aagtgcttat tatccataag tattttagct gctcctaaga gcattgctgt
     1501 gtgattatcg tgtccacaac tatgagatgc atctttattc ttacttttaa aaggtaaatc
     1561 cgtctcctcc ataataggaa gtgcatccat atctgccctt aataataagg tctttccacc
     1621 attttcactg tctaaaacag ctactattcc attattagac gtcaatttcg gagaatatcc
     1681 cattttttct aaagtactgg taacaaaggt tactgttttc ggtagattgc aaccaatttc
     1741 gggattttca tgtaaatatc ttctccatct aataatttcc ccttctattg actgcgcttc
     1801 tttaaaaaaa tttttcatat tccttcctcc taatcagata gtcagcaaaa tctcactttc
     1861 ttttagtaat gaaatgatgc tgatataaaa attataattt tatatattaa acttcatgtc
     1921 tttttaagga gttggaatat catctggaat aggacattta tatgttttac cattcataga
     1981 tttatcaaac tcttcttttg cctttttagc tatctctgga ttatttaata ttttggctcc
     2041 aaacatagcc atgatttttg atgcatatat catacctttt tctcctatag aacttcctgc
     2101 acaagatgta aactgccagc tatgacctgg tgcacctaga ttcgtacacc ctgtaaaaaa
     2161 gtatgctgtc ggaactatat gcattacatc tccaatatca gtagaaccat aactattaaa
     2221 gtttgtaaca tttcctccac cataatatag atgagttcct tctggcaaat tatatttact
     2281 tatcatagcc ttatattgtt gtgggctttg tctatctaat tcagctgcaa aatccaattc
     2341 ctctcgagtc caaggctctt gtttaatctc attcatacat tctgatacaa gatttgctaa
     2401 aacatgatta tttaaagtat tataacaacc tcctaaaaac tctacttcaa cttcagtttc
     2461 agtcatcatt gcagctcctt ttgctacctt tacaagtctt tcatatgtac tttctacagc
     2521 ttctctactt aaagctcttg tataatacca tactgatgct ttatcaggaa ctatgttggg
     2581 tgctactcct ccatctgtaa ttgtataatg aatccttaca tcagaagtaa catgttctct
     2641 caaataatta gctcccacat tagtaagttc cacagcatct agtgcacttc ttccattttg
     2701 agggtctgag cctgcatgtg cagtctttcc cttaaagtga aattttacag agttaacagc
     2761 tgttgaagta ccaattgtag cttcactaat tttatttgga tgaaaattta tagctaagtc
     2821 taacccttca aatgctccac ctctagccat aaatccttta cctgttaatt gttcttctcc
     2881 tggacatgca taaaatacta tagtcccact taagttattt tctagcatct ctcttttaag
     2941 tcctactact gcccctacat gtgctgtaca taataaatta tgcccacatc catgaccata
     3001 tgcactcaat tcaaatgctt cttgctttgt tgaaactttt tgatttaaac caggtaatgc
     3061 atcaaattct cccataaaac ctataactgg tttacctgaa ccaaaactag ctctaatagc
     3121 cgtggctaaa cctcctacac ctacttccac atcaaatcct tcacttctta gaaagtttgc
     3181 tgtattttca catgctttaa attctttaaa tgctttttct gggttttccc atattttctt
     3241 acttaaatca tcaagtttga atcttttttt atctatctca tttatagata tacttcctat
     3301 catttcttat tcctcctata agtatattta ctatcaacca aatataattg gacctattat
     3361 actagcaaat gccactgatg caatggttac agtaacaaat cctcctataa tcatctttgg
     3421 taataaataa tctgttgccc tttgtttttc ttcttcagtt ccttccatag catcaatacc
     3481 ttcgttggaa attatctgtg ttgcaggata agctagcata caagttacac caattgcagc
     3541 tgacattctt ggagaatatc ccaatagttt tcctacaatt gcagtaaata atattataaa
     3601 tacagaacct acaacaagta ttcctatagt tggaacaatc atcccaaaca atgctgttgg
     3661 agttacctta gcaagtcctc caggtaacat caacatacaa ccaaacatta taattccatt
     3721 agattgagat ttatctaaaa ttcctttttc taaaaaacct attcgtgtaa aaatcaaccc
     3781 tactaacaaa catgctatat taggatttaa ataatagttt gcaggggtaa atcctggtat
     3841 taaagtagcc aatcctacaa aattaccaat catagcaaca aatgaaagct ttgcaagata
     3901 cactagagta gatgattttt tacctaaatc ttctttaaaa attctcatac ttggaagtct
     3961 aaaattactt tttgactcat ctttcaaaaa atccccgtta tctcttttaa attttaattc
     4021 tttatttata caatatgtag ttatcggaat gccaaagaac ttttgaaatg ataatactag
     4081 aattacaaat gcagcaagtt caggtctatt tgcagcttct gcaacagttg ttacgataat
     4141 accggcaaca gtagaacctg ctattggagg tgctgctatt aaagcatact ctctaccaaa
     4201 taacatacta cccactgtta aacagccaac ccctattcct acaataccta ctaatgcaat
     4261 tacaaccgtt ttccattcat gacataagtc ttcaagattt ataagtgttc ctaagttagt
     4321 tattaataaa gctataccaa agttgctcat tgcagctaat atccctgatg ctcctgtaat
     4381 gtcttttggt aaaacatttg tccaaaatcc tagtaaaaat ataatacata caaataacaa
     4441 tgttgaaatt gcaccttttg ttttcttaga taaaaactca ctaaaagcat aaatcattac
     4501 acatacagta aagcaagtaa gtggattcca aaaattttcc attgttgttt cctcctaaat
     4561 ataataattt tttatcaata aaacaataat tataaaatgt atattatgca gttattctta
     4621 actacatatg gtatagcttg atattttttg atgatattat atacgtctta aattattcct
     4681 aaaatatcta aataaatata actagagtta actctctaaa tattaactat aattatcaac
     4741 ttactagatt tctttttgat ttttactttc tacataattt tacaagttag aattgtactt
     4801 caactacttt ctat
//
LOCUS       DAELXX010000182         4779 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.184, whole genome shotgun sequence.
ACCESSION   DAELXX010000182 DAELXX010000000
VERSION     DAELXX010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4779
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.184"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(46..483)
                     /locus_tag="KPH29_003520"
     CDS             complement(46..483)
                     /locus_tag="KPH29_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HBG1469081.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSTPLLDIKGK
                     KSGEIYKLNVCDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKEKLNI"
     gene            complement(740..889)
                     /locus_tag="KPH29_003521"
     CDS             complement(740..889)
                     /locus_tag="KPH29_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HBG1469082.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            complement(902..1132)
                     /locus_tag="KPH29_003522"
     CDS             complement(902..1132)
                     /locus_tag="KPH29_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HBG1469083.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
     gene            complement(1824..2045)
                     /locus_tag="KPH29_003523"
     CDS             complement(1824..2045)
                     /locus_tag="KPH29_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HBG1469084.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
     gene            2625..2882
                     /locus_tag="KPH29_003524"
     CDS             2625..2882
                     /locus_tag="KPH29_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG1469085.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENNV"
     gene            3331..4569
                     /locus_tag="KPH29_003525"
     CDS             3331..4569
                     /locus_tag="KPH29_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBG1469086.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIEFDCDSKDNSFNGDINVETVHCINEYFSDDDVE
                     HNYNNFHNKDTIKDKAISNISTIKKYIDESFDTNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISVLSDKGMTNKKNYFNHNHGIQKRNKSATKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYTTYDG
                     SVRERKKYENVRYWNPICEI"
BASE COUNT         1845 a          477 c          672 g         1785 t
ORIGIN      
        1 ataaatttgc aattagtgat attaataaat tactttagat ttttattaaa tgtttagctt
       61 ttctttaata ttttttatat aacgacgatt tataataact ttttcgttgt tttttaaagt
      121 agcttgcatg cgattatgtg atattacttt tatacttttt atgtagtcca tatttaaaat
      181 catggattta gatattcgtg agaaggatgt attagctaat aattttgata atttatatat
      241 ttttaaatca atttcaaata catcatcttc ataataagca aatgttttat ttccaactgt
      301 ttcaaagtat aaaatatcac agacattgag tttataaatc tctcctgatt ttttaccttt
      361 tatatcaagt aatggtgtac tcaataatga aattattttt tcaatgttat tatctagagc
      421 agaacatttt atggttattt caatatcatc acaattaata ccttgttcga catgtatttt
      481 cattaatcat cacctcattt tattactgtc attagaatag cattatatct tattgagtcc
      541 aactagatgc gttctaaatt tcaaattaag catagtgaaa ttcaaaaata aaatagtgaa
      601 attcaaattt gaggtggtaa aattcaaaaa aatactatgg atatttggat aaattatcca
      661 aatatcctag tatttagcat agaatatgtc taattatttt acaaatagac ctattttatg
      721 acttacatat attgtcaaat tacttcttaa atatcaaaat atttttggat aatgtatcaa
      781 tactgtttga taaagtattt aatttaccct caattctaac caacaagtac atggatagag
      841 ctataggaaa accaacattg gttattaaca gttgtaaact atcatccata aaattagccc
      901 cctattctat aactaagtca taaggagtgg tatttgtttc cacaaccttg gcttcaacaa
      961 cagaagctag gtcaaatcct ccaaccgaaa aaatattttt agatactatc aaatccatga
     1021 cagattttat attttcttca gttaaatcat cctttggatt ttctatagaa aaagaaactt
     1081 gtttatcctt tacattttta aagctcatga ctaatttttt cttaatttcc atataattac
     1141 ctccgatata ataatttata ttttaattgt ttgcttgtta atattggtat tttaattgtc
     1201 aatattagtg ttttgcttgt tagtattaat atttcaattg tcaatattaa tgttttactt
     1261 gttagtatta gtatttcaat tgtcaatatt agtgttttgc ttgttagtat taatatttca
     1321 attgtcaata ttagtgtttt gcttgttagt attaatattt caattgtcaa tattagtgtt
     1381 ttgcttgtta gtattagtat ttcaattgtc aatattagtg ttttgcttgt tagtattagt
     1441 atttcaattg tcaatattag tgttttgttt gttagtatca gtgtttcaat tgtcaatatt
     1501 agtgttttgt ttgttagtat cagtgtttca attgtcaata ttagtatttt aattgttgac
     1561 tctagttatt gctgatatag aaaatattta atttatttca attaattaga ttaaagtagg
     1621 gtagcattta aaatagctaa catgtttaag ttgaaatacg tttaatatat taatttttca
     1681 aagcattaat atattaaagt tataaccaaa attgaagtta taatctgcca aatctatagc
     1741 gtattaagtg cgttatataa cttaatcttt tatataaaga gttaattttt ttaatttgta
     1801 aagcagagta cgtttaaaag atactattta gataatgatg tactatctaa ttttaaaata
     1861 tctacaatag ggttctcttg aagacttgct attgcttctg caacagcata aacatcttca
     1921 tttaatgcct ctgcttttac atttgcaaat gatttacttt taacaactgc cttaccctta
     1981 tcatcaaaac cagcatccaa ttttagtttt atactagata aattttttgt tgaaacaact
     2041 tccataaaat tacctcctaa aaatttaatt tcaaagattt gtcgactttc tttgttagct
     2101 ataatactat catctaagat aatatcggag ttaaacagta atttctaaag attttttgtg
     2161 taaattttta taggaaaaat tttacttttc tgatgatgaa aaagtttaca agttattttt
     2221 gaagatatgt tcttagtgtt ttgaaatagt atttgtatat attcatgctt agttgagata
     2281 gttttgtatg gattttcata tgtgtagagt taaatgttag gtaccaaagt ttatgttaaa
     2341 ttaaatgtta aagcttatgt taaaaatttt attaaattta atctgtaaat aaaaaagtta
     2401 aaagtccaat attaacattt tacttttaaa ccattaaaat ctttattaaa gctaacaaag
     2461 ttataagtta tgagaaattt tattaaagtt aatacaaaaa ttaattttaa agctaagaaa
     2521 ttttttatta gattcaataa aactaataaa tatagtttag tcaataatgt attaaataaa
     2581 aactttgtta agtctataaa aatatcttaa aggaggtgta acttatgaag ataaaagtgg
     2641 aggatatacc agttcaattt catgcaatgg tagaaataat cggaattgat aagttcatag
     2701 aggttgccaa gttgtatgga ggaacaaata cttatatacc tacgtataag gggcttttta
     2761 gatatgctag aaatagggat atagtaaaac aatttaatgg agtcaaccat aatgaattgg
     2821 ctattagata taatatgtgt gtaagcaata ttaagcgaat actcaatgaa aataatgttt
     2881 aaacataatg aaaatagcct tgttaaatgt aagagtaaag acaaagaaga tgttttataa
     2941 agttttttta gacaaattaa ctaaatttac tacttattaa tttaaataca ttgtatattc
     3001 tataaaaaaa tttgttaaat actggagata tattgagaat acaaagaaaa tggtcgaaaa
     3061 tatttgttgg ctaaaaaata gataaaatga tacaattgct tgtataaagg ggaaaaataa
     3121 ataaataaac tatataaata aagaaatagc aattaatatg acataatata tttataaaat
     3181 taagaatata aatagatatt ttaagggatt agttgttaaa ttaggaaata ttaaaaaaat
     3241 atatatattt atatttaaac tattttgtta tttatataaa aatacttatc tagttactag
     3301 caattattag tagaaatggg ggaaatagag atgaataagg gattttacaa attaaattca
     3361 gtggtattga actttgatat taagaatgat gttaacaaga taagatttaa tcattatata
     3421 atgagaaaag aaaacataca aagacttcaa ggaactctgc caaacggaca attttatatg
     3481 acagtgagaa gagctgctac agatttacaa ctttctattt ctactataag taggcttgta
     3541 aaagagtttt tggatttagg gataataaga ttagtgtcta agggtgtaaa aggtaatcgt
     3601 tctgtatata gttacgtaag tatagagttt aatggaagtt tatttgatga tagcaaatat
     3661 atggaacttg actgtgtatg taatgatgaa tttattgata atagtggcat agagtttgat
     3721 tgtgatagca aagataattc ttttaatgga gatataaatg tagaaactgt gcattgcatt
     3781 aatgagtatt tctctgatga tgatgtagag cataattata acaatttcca taataaagat
     3841 actataaagg ataaagccat ttctaatatt agcactatca aaaaatatat tgatgaatct
     3901 tttgatacaa ataaatcaga aaattctaac ttacaacagg agtcaataaa tttcaacttt
     3961 aatgatacat gcaatgaaat agataaaaga acgaataata gtatagaaaa taatcaaaat
     4021 aaaaggccta tgagattgag tgacatcagt gttttaagtg ataaaggtat gacaaataaa
     4081 aagaattact ttaatcataa tcatggtata caaaaaagga ataaatctgc aacaaagaaa
     4141 aaagaatcct taaataaatt taataaaaaa aatatacagc aaagcattat aaatatgcta
     4201 aataaaaaat caggtaagaa ttttaaaatc gattctccaa taagcataaa acttatcaat
     4261 gaaaggctaa aggagggcta tgtcttagaa gatttctata aagttattga ggtaaaagtg
     4321 gctaagtgga aaaataccat aatggaaatg tatataagac cagaaacctt gtttagctat
     4381 aagtttgaat cctatgtaaa tgaaaacata tctatgaaaa gtcaaagtaa tgttcatcca
     4441 tgtaaagaag gcaatagtaa tgatagcagt aattatatac attctgatta tacaacatat
     4501 gatggcagtg tgcgtgagag aaaaaaatat gagaatgtaa gatactggaa tcctatatgt
     4561 gaaatttaat tagtttaaga atgtatttat gataaataag atattaatag agaatagtga
     4621 gctatagcaa atgatatatc aatagttggt agtaagttat attaagcaag atattactat
     4681 caggtagtga attatagtga acgatatatc aataattggt agtaagttat atcaaataag
     4741 atattactat cgagtaatga attatagtga acgatatat
//
LOCUS       DAELXX010000183         4691 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.185, whole genome shotgun sequence.
ACCESSION   DAELXX010000183 DAELXX010000000
VERSION     DAELXX010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4691
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.185"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(292..759)
                     /locus_tag="KPH29_003526"
     CDS             complement(292..759)
                     /locus_tag="KPH29_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HBG1469087.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKSEKWVDVCKNW"
     gene            complement(838..1380)
                     /locus_tag="KPH29_003527"
     CDS             complement(838..1380)
                     /locus_tag="KPH29_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1469088.1"
                     /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDIGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF
                     NEKNSITESTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
     gene            complement(1432..2118)
                     /locus_tag="KPH29_003528"
     CDS             complement(1432..2118)
                     /locus_tag="KPH29_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBG1469089.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTDIANTKYINLYSMTFLTIIIFIISMYFSI
                     KISKKNKFNY"
     gene            complement(2106..2978)
                     /locus_tag="KPH29_003529"
     CDS             complement(2106..2978)
                     /locus_tag="KPH29_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1469090.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDREKFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVRGCSI"
     gene            3113..3913
                     /locus_tag="KPH29_003530"
     CDS             3113..3913
                     /locus_tag="KPH29_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HBG1469091.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            3938..4507
                     /gene="yfcE"
                     /locus_tag="KPH29_003531"
     CDS             3938..4507
                     /gene="yfcE"
                     /locus_tag="KPH29_003531"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBG1469092.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
BASE COUNT         1686 a          725 c          504 g         1776 t
ORIGIN      
        1 actatttttt atttttaaat atcttacatc tataatagta acactcaaag gataattttt
       61 caataatttc taattatttt tttaacaata tttattacat tttttccact tttttgtcat
      121 ttttttattg tttcgaaaaa aaatcagaat tttttaactt tattttggat tattagttta
      181 aattagattt tcctcttaga tttataaata tttacacaaa aaactacctc tgtatagatt
      241 gtatacagag gtagtttttt actaattcct actatagttt actatatttt tttaccaatt
      301 cttacaaaca tctacccatt tttcactctt agaaaacttc tccatttctt cacatgtaag
      361 ttctattgca gagttactac tgccacatgc aggaaatact gtatcaaatc tcttcattga
      421 atcatctaaa tagactttta tagaattttt taaaccaaat ggacagactc caccaatagc
      481 atgtccagta aactccaaga cttcttctgg tgtcaacatt ttagccttac aattaaattc
      541 agccttatac ttcttattat caatcttagc atcaccagct gtcacaatta aaattgcatc
      601 atctcctatt ttaaatgaga gtgtcttagc tattcttgct ggaataaccc cagctgcttc
      661 agctgcaagt tccacagtag cactagattg ttcaaattct aatatactgt cttccttgcc
      721 aaattgttta aaatactctc ttacattact tattgacatt aaatacccct cctagtctga
      781 atttttataa atttcatatt attcttaatt atatctataa ttgatttatt tttcaaatta
      841 ttccataata ttagcattcc atgtatcatt attttttcca actactagat aaagtccttt
      901 tgccattgga atattgacaa tatattctcc tttatattct acactcatac cagattcatt
      961 aaagtaccta taatcaaaat ttagtccttt tggaattaca aaatcaacat aaccttgatt
     1021 atttggtttt attccatatt tcagagtact ttcagttata gaattttttt cattaaataa
     1081 atctacattt acatatgcag aacacttatt cattcccttt ttattttcca cacaattttc
     1141 aaacttcatt ttagcactgt ttaatttata actatattta ctattattgc ttattttaaa
     1201 tcttacaata gtaccgatat ctctacttct cctgctatca aaatcaaaaa caatttcctc
     1261 taaatgattt atttcataat ctttgacact taccatatct ctatatacta atttatttat
     1321 agctatgaat agtgtaaaaa tccccaaaat aaaaaatgaa acttttataa acttcttcat
     1381 ttttagtctc cttatatttt gtgctattgt gctataatat taattaaaat tttaataatt
     1441 aaacttattc ttttttgaaa tcttaataga aaaatacatt gatattataa aaattattat
     1501 tgttaaaaat gtcatactat ataaatttat atacttagta ttagctatat cagttacata
     1561 atttgaaaga tatactcctc caactttact ataattaaaa tgctttactt gatatacaat
     1621 catactaatc atcttcaaaa gacttccata tacaaagtaa attaaaatac ttactataaa
     1681 accacaaact ttcatttttc catatccaaa cttaaaaatt aaaggcagta aaatcgtcaa
     1741 aattgctaaa aatatattta cagaaaaaaa tacatcattt aaaaccatat ttctaaagaa
     1801 cactagtgat gtcaatttac ttagtaagga cacaattatt gttacagaaa ttattagtcc
     1861 tacacctaat aaatattttg agtaaactat atcttctata ctaactggca tactccttat
     1921 aaaaatcctt gaatcatttt cataatcaca ctcaaatgta tttataaata tcaaataact
     1981 aacaacaata cttactgtat aatatgaaat tgaattaagg aaaaaataaa acacaaaaaa
     2041 tagcaatatg tactttaaat tacggatacc atctgaacta attcctatta ttaaatcttt
     2101 tcttattaaa ttgaacatcc tctcacctca tttacataat aatacattag ttcttctata
     2161 gtaggatttt tgaccttatg cttatcaatt tctatcaata ggctttttac tttcaagcta
     2221 tctccaactt taacaagtgc ctcattataa tatttatact ctttaacacc aactatactt
     2281 tctttaattg cttctaactc tgctttattt gccctaatta tcttatattc ttgttctatt
     2341 aattctttct taacagtaaa aatctgattt cctctattta taaaaacaag ataatcagca
     2401 attttaatta aattatcagt attatgagta gaaattatta cactactact tccatttttc
     2461 acaaaatctt gtaagtactg cattatttca gttcttacaa tcgggtctat accagcagtt
     2521 ggttcatcca aaatcaataa cttagcatta tgactaagtg cgcttgataa cataagcttc
     2581 tcagattgac ctttagataa attttcaata tgcatatttt cttctatgtc aaacttatta
     2641 aggtattcat caaatttttc tctatcaaaa ttcttataaa ataaagaaac tatttttcta
     2701 aattccttaa cctttaaatg tggataaaat tgtaaagaat catatacaaa accaatattg
     2761 tttttaaagc ttattggaga attttcaata gtttcattac aaagttcaat ttcaccttca
     2821 tctgcatcaa taatccccat tataattttt attaaagtcg tttttcccga accatttggt
     2881 cctataatcc caacaatatc cccttccttt aactctaagt ttatattatt taacttgaat
     2941 tttcctaatt tttttgtgat attccttatg ctaaacacat cttcacctct tatgcatttt
     3001 tttataccca cctatacata ttttaacaca ttcactacaa aggtcattta taaatttata
     3061 aattaagaat tataaaacta tgttataata caaagtatga agaggtgaat atatgaaaag
     3121 aattataact ataaatacaa accaattaaa taagtccctt gagactaaag aactcttaac
     3181 tagaaaactt atcaatgctg gctttgaagt gtattcagat atttatccag acacagagct
     3241 tattatatca attggtggtg atggctcttt tttaagaaca gttagagatt tcgattttcc
     3301 tgagattccc ataatgggaa taaatacagg tcatctgggg ttttttcctg atattttacc
     3361 agataagatt gatagcttta ttgaagctta tacaaaaaaa gattatatta ttcaagaaat
     3421 gtcacttctt aatgcggaag tatatacaac tactagtggt tctaatatgc ttgctgtaaa
     3481 tgaggttgtt ataagaggag acaagtctag gactatacac ctaaatttaa gccttgataa
     3541 taaacatata caaaatttta gtggagatgg tatgattatt tctacttcca caggttcaac
     3601 agcctataac tattctgctg gaggtagtat tgttgatata aatcttgaac ttatgcagat
     3661 tacaccttta catccgataa ataccaatgc atataggtgc tttacatcaa gtataatatg
     3721 ttcaaatgaa tctgttataa agattgcacc agaatataga tttgaagatt ctgtattgat
     3781 tgtcgttgat ggagttgagc acagatttag acaaatcgaa aatataaaag ttagtatatc
     3841 tgatgcaaaa ataaaattac ttagaatgtc aaactatgag ttttggcata gagtatctga
     3901 aaaattttta tagactgtac ttaggaggat ttttaatatg aaaataggaa taatgagtga
     3961 tactcatggt agcttacttt actttgaaaa agcgttaaat gtattatctg attgtgatgt
     4021 tttattacat ggtggagatg tcttatatca tggacctaga aatgatattc cagaaggata
     4081 taatcctaaa aaatttattg agacattaaa taaattagaa aacattgtga ttgtaaaagg
     4141 gaactgtgat gcagatgttg accagatggt tatagaacat cctattcaga gtccttatgt
     4201 catgagccaa tttggtgaaa ttagaattat attgaatcat ggttatatag aatcagaaga
     4261 agaaattatt gacaaagcaa agaaaatggg tggtgacata ttagttttag gacatactca
     4321 tgtaaaaaaa ctatacatgg atgataattt aatagtaata aatccaggaa gtacttctat
     4381 cccaaaagat ggaagccatt ctgtcgcaat tatagatatc ataaaaactg atgatgagga
     4441 tgagcttgaa cttgatataa acttaattga catcaatact ggaaatataa taaatatcaa
     4501 tgattagtaa atttttaata aaaataaaat aagctttaat ttattaaact cataataaaa
     4561 acaagtaaac cattttattt ctatctcaat tagaagttta agtacaatac cttaaacttc
     4621 taattaaata gaatctggtt tacttgtttt attatatgct atattgaaat ataacttagt
     4681 ttacttgttt c
//
LOCUS       DAELXX010000184         4684 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.186, whole genome shotgun sequence.
ACCESSION   DAELXX010000184 DAELXX010000000
VERSION     DAELXX010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.186"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..239
                     /locus_tag="KPH29_003532"
     CDS             <1..239
                     /locus_tag="KPH29_003532"
                     /inference="COORDINATES: protein motif:HMM:NF014225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1469093.1"
                     /translation="VEESRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKIRRTAIEF
                     STEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
     gene            497..1546
                     /gene="carA"
                     /locus_tag="KPH29_003533"
     CDS             497..1546
                     /gene="carA"
                     /locus_tag="KPH29_003533"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBG1469094.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            1739..>4684
                     /gene="carB"
                     /locus_tag="KPH29_003534"
     CDS             1739..>4684
                     /gene="carB"
                     /locus_tag="KPH29_003534"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBG1469095.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYLDDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTAKSLRENG"
BASE COUNT         1929 a          478 c          936 g         1341 t
ORIGIN      
        1 gagtagaaga atctagacca aacatattag atgctataag aaataaacaa gttgatatag
       61 taataaatac accaactaaa ggaaatgact ctacaagaga tggatttaag ataagaagaa
      121 cagctattga attttctact gaaataatga catctttgga tacactaaaa gctttagtag
      181 aagttaagaa gaaacattta aataaagatg agttaaaagt ttacaatata gctgaataga
      241 aattaagttg attgtatagt ttaagaattt aattattaaa taaaaaaaaa gaggttattt
      301 tttctaggag tttttacttt tgagataact tctttttttt aagaaatttg tagtataata
      361 gaaaaagata aaaaataaat gtaaataagt ataaaactag aatgtaaaag tgaaaaatag
      421 atatagatat ttaaaagctt atatttagtt aatgttcttt agtttgtcta aagagaatta
      481 taggaggtac tgtagtatga aagcaaggtt aatattagaa gatggaacag tttttatagg
      541 aaaggcattt ggatatttag aagaaagtgt aggagaagta gtatttaata cttcgatgat
      601 aggatatggt gaagttttaa cagacccttc ttactatggt caaatagtta ctatgactta
      661 tcctcttgta ggaaattatg gtataaattt atcatcagca gagtctgaaa aagttcaagt
      721 aaagggattc atagtaaggg agaaaagtga ttctccaagt aattttagat gtgaaattga
      781 tatagaccaa tatttaaagc aaaataaagt tatcggactt gaaggaatag atacaagagc
      841 tttgactaag atacttagaa ataatggaac aatgaaaggt ataattacat tagaagacag
      901 taaattagaa gatgttaaac ataaattgga taagttttca aatacagaag cagttagaac
      961 tgttacaaga aaagaagtag agcatataaa aggaaatggt ccaaaagtgg ctgttatgga
     1021 ttttggagta aaaagaaata tattaagatc atttatagct cgtggatgtg atataacaat
     1081 atttccagct acaacatctc cagaagatgt attgagtata aaccctgact taatattctt
     1141 gtcaaatgga cctggagacc cagaagacct agaagatgtt atagaaaata taaaagctct
     1201 tattggtaaa aaacctatag ttggaatatg cttaggacat caacttttag ctttagctct
     1261 tggaggaaaa actgctaaac ttaaatttgg gcatagaggt ggaaatcatc cagttaaaga
     1321 tttagaagaa ggaaaagtat ttataacttc tcaaaatcat ggttattatg tttcagaagt
     1381 tcctgaacaa atgaaagtga cacatataaa tttaaatgat aatactgttg aaggaatgag
     1441 acatgagaag ctagatgtat acagtgttca ataccatcct gaagcttgcc caggaccaaa
     1501 agataatgac tatatttttg ataaattttt agaattagta aaataaataa cattagaata
     1561 gctaactaaa aaacaagaat aacttaaatc aaaaagtttg gtttaattaa catagatgta
     1621 ttaaatagat gtattgatga gtaaatttga acttaagata aaataaagta attaaagttg
     1681 attgtataga tgatgaagat aagaactgtg aaaattgttt agataaggag aaagagttat
     1741 gcctaaatta gatagtataa agaaaacttt agtattaggg tcagggccaa taataatagg
     1801 tcaagctgca gagtttgact attcaggaac acaggcctgc caagcattaa aagaagaggg
     1861 aattgaagtt gtattagtaa atagtaaccc agctactata atgactgata aagaaattgc
     1921 agataaaata tatatagaac cattaacaat agaatttata gaaaaaataa tagaaaaaga
     1981 aagaccagac agtttacttg caggtatggg tggtcagaca ggattaaact tagctgtaga
     2041 attacatgat gctggtatat tagataaata taatgtaaaa gttataggaa catctataga
     2101 atctataaaa aagggcgaag atagagattt atttagagaa gtaatgaaag aaataaatca
     2161 accagtaata gttagtgata tagttacaaa tttagaagca ggtcttgagt ttgccaataa
     2221 gataggatat ccagtagttg taagaccagc atatacgcta ggtggaactg gtggaggtat
     2281 agctgatact gaggaagaat taagagaaat actttcacat ggactacagc taagtcctgt
     2341 tggacaagtt cttcttgaaa agagtataaa aggttggaaa gaaatagaat atgaagtaat
     2401 gagagatgga aatggaaact gtataactgt atgtaatatg gaaaatgtag acccagttgg
     2461 agttcataca ggagacagta tagttgttgc tccaagtcaa actctaagtg atgaagagta
     2521 tcaactactt agaaaggcat caatagacat aataaatgca atagaggttc aaggtggatg
     2581 taacgttcaa atagctttaa acccacatag tttagaatat gcgataatag aaataaaccc
     2641 aagagttagt aggtcatcag ccctagcatc aaaagctaca ggatacccaa tagcaaaagt
     2701 agcagctaag atagctttag gttatacttt ggatgagata gaaaatgcag taactaaaaa
     2761 aacttatgct tgttttgagc caacattaga ttatgtggta gtaaaaatac caaagtggcc
     2821 atttgataaa tttagacatg caaatagaaa attaggaact aagatgatgg caactggtga
     2881 aatcatgagt ataggaagta actttgaggc tgctattctt aaagggataa gatcacttga
     2941 aacaggaaaa tattcattgg ttcatgctcc atcagaaggt agaacattag aagaattaaa
     3001 ggctagagta gtagtacctg atgatgagag actatttgac ttagctgaaa tgataagaag
     3061 aggctataaa gtagaaatga tatcagaaat aactggtgtt gataagtggt ttataaataa
     3121 atttaaatgg atagtagaac aagaagaaaa gttaaaagtt ctaaaaatag aagacttaga
     3181 taaagaatat ttacatgaat taaagaaaaa gggattttct gataaaggta tatctgactt
     3241 aatgaagata agcccagaaa aattatatga attgagaagt ttatataata tacaaccagt
     3301 ttataaaatg gttgatacat gtggaggaga atttgaagct ctatcacctt attactattc
     3361 aacttatgaa caatatgatg aggtagtagt tagtgataag agaaaagttg tagtattggg
     3421 ttctggtcca ataagaatag gtcaaggtat agaatttgac tattgttcag ttcactgtgt
     3481 aaaatcttta agaaaaatgg gaatagaaac tataattgta aacaataacc ctgaaactgt
     3541 aagtacggat tttgatacat ctgataagtt gtattttgaa ccattaacag aagaagaagt
     3601 tttaaatata atagaaaaag aaaaaccaga aggtgtaata ctacagtttg gtggacaaac
     3661 agctataaaa ttggctaagt ttttacatga aaaaaatata cctatactgg gaacagactt
     3721 tagagatata gatgcagcag aagatagaga aaaatttgat gatttactag agagactaga
     3781 tataaataga ccaaaaggaa aaggtgtatg gacaactaat gaaggtgttg aaattgctaa
     3841 ggagttaggt tatccagtat tagttagacc atcttatgta cttggtggtc aaggtatgga
     3901 aataacttat aacgaagaaa aactaattca gtatttggat gatgcatttg atagagacca
     3961 taaaaatcca gtacttatag ataaatatct aactggtaga gaaatagaag tagatgctat
     4021 atgtgataag gaagatatat tgatacctgg tataatggaa catttagaaa gagctggagt
     4081 tcactctgga gatagtacaa caatgtatcc aagtcaaaat atatctgatg aaataaaaga
     4141 aaagatagta gaatacacta aaaaaatagc tttagacctt aatgtgcttg gtatggttaa
     4201 tatacaattt atagaatttc aaaatgaatt atatataatt gaggttaacc ctagagctag
     4261 tagaacagtt ccatatataa gtaaagtaag tggagtacca atagttgact tggctactaa
     4321 gtgtatgtta ggagctaaat taaaggattt aggatatggt acaggagttt acaaagagcc
     4381 aaaattagta tcagtaaaag taccagtatt ctcaatgtct aaattatcta aagttgaagt
     4441 aagtctagga cctgaaatga agtctacagg agaagtccta ggtgtaggag aaaatttaga
     4501 agaagcctta tataaagggt tcttggcagc aggtagacat atgtctgatg aaagaggagt
     4561 agtacttgct actgtaaata atcatgataa agatgagttt atagagatag caaaagatat
     4621 gaaagaatta ggatatactt ttgttgctac agaaggaaca gcgaagagtt taagagaaaa
     4681 tgga
//
LOCUS       DAELXX010000185         4654 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.187, whole genome shotgun sequence.
ACCESSION   DAELXX010000185 DAELXX010000000
VERSION     DAELXX010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.187"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            154..1092
                     /locus_tag="KPH29_003535"
     CDS             154..1092
                     /locus_tag="KPH29_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBG1469096.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKNLK
                     NAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNSKR"
     gene            1568..1996
                     /locus_tag="KPH29_003536"
     CDS             1568..1996
                     /locus_tag="KPH29_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBG1469097.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKTLAE"
     gene            complement(2149..2943)
                     /locus_tag="KPH29_003537"
     CDS             complement(2149..2943)
                     /locus_tag="KPH29_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase"
                     /protein_id="HBG1469098.1"
                     /translation="MEKYATMIKEKLNEGKVLCGVAVSLSDSVVSELIGISGYDFIWI
                     EGEHSALDRKDIQQHMIAAHAGGAASLVRLVNSDPDLVKPIMDMGTDIICFPFINTVE
                     DAQKAVAACTYPPNGIRGYNPQRASYYGNMNYFDYVKNANNETLKMMLIEQKAGYDNL
                     EEIVKVEGIDIIALGPGDLSLDMGFSGYMNRIEIKDMISHAAEICKKYKMPFIIFPTI
                     EKKSIEEWVNIGANMLCFAQDTTFITYVINQLLSFYNEIVPIQKQH"
     gene            3092..3460
                     /locus_tag="KPH29_003538"
     CDS             3092..3460
                     /locus_tag="KPH29_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG1469099.1"
                     /translation="MKYSITDVSKITKISSSAIRFYEEKGLLLPINRKPSGVRYFSEE
                     DVERLIFIKELKNTGLSIKDIHECFHFCDMGDGTIDKRIQLFQQHKQNIKNKIHELES
                     CMDKIDEKIYYLKEKKMCKK"
     gene            3689..>4654
                     /locus_tag="KPH29_003539"
     CDS             3689..>4654
                     /locus_tag="KPH29_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="HBG1469100.1"
                     /translation="MVDFIESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     CQEKHFHLSEEQLLYVIQGEGIQMIDGKKVNIKETSIVYCPPYSEHEIINTGKIDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDLKYKGI
                     FETKKENKFCDICKNIKPCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     IECGNFIVYKSIDIDKNLSELSSSSGIDIKYINKIYNEFPLNPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKSRKENIRLEEALKKANSRLLE"
BASE COUNT         1862 a          498 c          774 g         1520 t
ORIGIN      
        1 attattttat atacaaaaaa atatgactcc taaaaataaa aaatatatca taaatataaa
       61 taagtagata taggttttaa tttgatttat aaacctatta taaaataaaa ttcatgattt
      121 ttataagcaa taaaattttt aggaggatat atgatgaata aaagaatgaa actaattccg
      181 tatgaaataa atgaaaatct aagaggtgca aaaaataaat tcccatatgg aataaaacaa
      241 atgaatgcta ggggaatgtg ggatgaaggt tatactggta aaaatattgt agttggtata
      301 atagatacag gttgtgatat atctcatcct cttttaaaag gaaaaataat tggtggtgca
      361 aattttagtg atgacagtaa tggaaataaa aatatatatg aggattttaa tggccatgga
      421 actcatgtgg cgggtattat agctgcatct aactataata atgaagttat gggagtagct
      481 ccagattgta aattattaat agcaaaagca ttaaataaag atggtaccgg aacatatcaa
      541 agtataatta atgctattaa ctttgctgta aataacaagg ttgatattat atctatgtct
      601 cttgggggaa acaaagatga taagaattta aaaaatgctg tcatgcaagc agtaaaaaat
      661 aatatttctg tagtgtgtgc agcaggtaat aatggagatg gtgattctag tacaagtgag
      721 tatagttatc cagccagtta tgctgaggta atagaagtag gtgcaataaa tgaaaactat
      781 ttggttgaaa agtttagtaa ttcaaatact acaatagatt tggtggctcc aggaagaaat
      841 attatatcga cttatatgga taataaactt gctattatga gtggtactag tatgagtgca
      901 ccatacgtat caggctcatt agcactaatt aaagaatggg caagagagga gtttgaaaga
      961 gatttagatg aagctgaact gtatgcacaa ttaataaaat gtacgagagc gcttggaata
     1021 cctagaacgg aacaaggaaa tggatactta tatttaaatc tttataaata caagaataat
     1081 agcaaaagat aatttttgat ttgatgataa aatagctata ttatatagag tcgagacaaa
     1141 taataaaaat tacttaggtg taagatttta catcaatgta taaaggtatt aagtgataaa
     1201 atttataaac atattagcta gttagaattg aaaaataaat atacgatatt atagatagca
     1261 catctggaaa aggtgtgttt ttctatgtac ttatacatat aaaagattta taagagatgc
     1321 aaaagtacta taagttagaa atttttctat gaaagattag aaatacgata cagtgttttg
     1381 atattataaa atgaatataa aatataaatg ataaatatta tcaataaata ttgaatttca
     1441 aaatcaattg tgatattcta tatttagaaa aataaagaga tttcagataa agaaatcttt
     1501 tgttttttag atgttagtta gctataacta actattgatt aattaaaaaa tactacagga
     1561 ggtaaaaatg agtaaaatat atattgttta ttggagtgga acaggaaata ctgaaaaaat
     1621 ggcaaatttt gtggctgaag gtgtgaagtt aaaaggtaag acaccagaag ttttagatgt
     1681 gagcttactg aaaccaagtg atttaaaaga agaagataaa tttgcattag gttgcccatc
     1741 tatgggagca gagcaactag aagaggggta tatggagcca tttgtttcag aattagaatc
     1801 tatggtatca ggtaaacaga ttggattatt tggttcatat ggatggggaa attgtgaatg
     1861 gatgagagac tgggaagaac gtatgcaaaa tgctggtgct acaattattg gtggagaagg
     1921 aattacagca atggaagacc caaatgaaga agcaaaagat gagtgtatag aattaggcaa
     1981 aacgttagct gaataaattt gtatattata aaaatagtat aaatagcaac taatgatgat
     2041 agcagtatat aataataaga gagactaata ttatgataca taagaaaata tccttaatag
     2101 agatagataa aatattagtc tcttttaata taaaattaga tttataattt aatgttgttt
     2161 ttgaattggt acaatctcat tataaaaaga taataattga ttgataacat aagtgataaa
     2221 ggtagtatct tgtgcaaaac aaagcatatt tgctccaata ttaacccatt cttcaatact
     2281 tttcttttct attgtaggaa aaataataaa gggcatctta tattttttgc atatttcagc
     2341 agcatgagaa atcatatctt ttatttcaat tctattcata tatccggaaa aacccatgtc
     2401 taaagataag tcaccagggc ctaatgctat aatatcaata ccctcgactt ttactatttc
     2461 ttcaagatta tcataacctg ctttctgttc aattaacatc atttttaatg tttcattgtt
     2521 tgcgtttttg acatagtcaa aataattcat attcccataa taagaggctc tttgaggatt
     2581 atatcctctt ataccgtttg gaggataagt acaagctgct acggcttttt gtgcatcttc
     2641 tactgtatta ataaatggga aacaaataat atctgttccc atatccatta tgggcttaac
     2701 aaggtcaggg tcactgttta ctaatcgtac caaagaagca gcaccaccag catgggcagc
     2761 tatcatatgt tgctgaatgt ctttacggtc taaagcacta tgttctcctt caatccaaat
     2821 aaaatcatat ccagatatac cgattaactc acttacaact gaatcagaca gtgaaacagc
     2881 tactccacat aagacctttc cttcatttag cttttcttta atcatagttg catatttttc
     2941 catttatatc atcctttctt ttgtaagttt tcttttacac ttcaaagagt acaacttcaa
     3001 cctaacttga agtcaatact tttttgttga ctactgtata aggtattttt ataatattct
     3061 tattcatata aaatttaggg aggtagagat tatgaaatat tctataactg atgtatcaaa
     3121 aataacaaaa atttcttcat ctgctattcg attttatgaa gaaaaaggtt tattacttcc
     3181 tatcaataga aaaccaagtg gtgttcgcta tttctctgaa gaagatgtag agcgtctaat
     3241 atttatcaaa gaattaaaaa atactggttt atctattaaa gatatccatg aatgctttca
     3301 tttttgtgat atgggagatg gtacaataga taaaagaatc caattatttc aacaacataa
     3361 gcaaaacatt aaaaataaaa tacatgaact agaaagttgt atggataaaa tagatgagaa
     3421 aatatattac ttaaaagaaa aaaagatgtg taaaaaataa tccttataga ataaagaaat
     3481 aattttttga tttttctaat ataaagagta aattcaattg gaaacatttg aggaaagatt
     3541 ttttacacta tatttttagg aagaattgga caatgttcta tataactatt tatctataca
     3601 ataaaaaaga aaattatgat aatgtatatt ataaggagat atttaaagga ataaaaaaat
     3661 atttagataa aaactacgag gtgagtttat ggtggatttt attgagtcaa ataatgaagt
     3721 acaaaaattt gattggggag aggttatgtg gatacacgaa ccatcaaaaa cacaattcaa
     3781 tagacttagt gctggaattg taagattttt tccgggaaac tgccaagaaa agcattttca
     3841 tttaagtgaa gaacagcttt tatatgtgat tcagggagaa ggaatacaaa tgattgatgg
     3901 taaaaaggtc aatataaagg aaacatctat agtttattgc ccaccatatt cagaacatga
     3961 aattataaac actggtaaaa ttgatttagt aatattaatt acttatgttc ctcataaatt
     4021 ttcgagtttg agacaaatac ctatagtatt ttctgaaaac aatatacaag agttagttaa
     4081 tgtaaacata attgaaaatc ttgcaaatca aatatctaat atattgaagc ttagaatatc
     4141 tgtatatgac ttaaagtata aaggaatatt tgagactaag aaagaaaata aattttgcga
     4201 tatttgtaaa aatataaaac cgtgtactaa aaaacttgtt aaaagcataa ataatgataa
     4261 ctttgaaaaa gtgtaccatt gtgagtatgg tgtttccagt ttagagatac ctatagtatt
     4321 agaagaaaat atagttggat atatagaatg tggtaatttt atagtatata agtctattga
     4381 tatagataaa aatttatctg aattatcaag tagttctggt attgatataa aatacataaa
     4441 caaaatttac aatgaatttc cattaaatcc taaaagtaga ttatatgtat taaaagaaaa
     4501 tttattaatg atgtctgaat ttattcagga aatagctaag aggaattttt ttgaaaatca
     4561 acttagtata aaagatgagg agatattaaa gagtagaaaa gaaaatataa gacttgaaga
     4621 ggcacttaaa aaagcaaata gtagattatt agaa
//
LOCUS       DAELXX010000186         4641 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.188, whole genome shotgun sequence.
ACCESSION   DAELXX010000186 DAELXX010000000
VERSION     DAELXX010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4641
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.188"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            230..373
                     /locus_tag="KPH29_003540"
     CDS             230..373
                     /locus_tag="KPH29_003540"
                     /inference="COORDINATES: protein motif:HMM:NF014609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBG1469101.1"
                     /translation="MNKKFRLYQVDSFTKERFTGNPAGVITNADGLSESQMQKITREL
                     NNS"
     gene            395..1150
                     /locus_tag="KPH29_003541"
     CDS             395..1150
                     /locus_tag="KPH29_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HBG1469102.1"
                     /translation="MAKNHQENYDVHIRFFTPTNEVPICGHATIAAHYVRAVENQFNN
                     KTVFQKTGAGILPVDIIKKDNDYEIIMTQGKIEVSPPLQQTYQKRLLNAFGILNEDIR
                     TDCPIAISSTGHSKVMIGIKELSTLNSLSPNLYELSKISQEIKCNGYYIFTLHNKETL
                     QVHGRMFAPAIGISEDPVTGNANGPLGAYLVHYKVIPIPNTEFIFEAVQGEAIKRKGK
                     MLVKVIISDNKPVEVKIIGKAVIAFSTNIEIEL"
     gene            1375..1986
                     /locus_tag="KPH29_003542"
     CDS             1375..1986
                     /locus_tag="KPH29_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBG1469103.1"
                     /translation="MINDIKNKFKNYKPYINGYKNMKRASVLIPIIEINNTHYILFEV
                     RSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVK
                     FIIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGS
                     IPNQKDYKFESGSYPILFYKYKDYVIWGMTAKILENFLNFLSE"
     gene            2284..2622
                     /locus_tag="KPH29_003543"
     CDS             2284..2622
                     /locus_tag="KPH29_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HBG1469104.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
     gene            complement(3029..4465)
                     /gene="miaB"
                     /locus_tag="KPH29_003544"
     CDS             complement(3029..4465)
                     /gene="miaB"
                     /locus_tag="KPH29_003544"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBG1469105.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
BASE COUNT         1690 a          644 c          543 g         1764 t
ORIGIN      
        1 ttagtatgca aaattctatg aagcaggaac cctgtgactt tagtcatggg aggttcagtg
       61 tagcatagag ccttacataa tactactttt ttagatgtat atttttaaat tttaatatag
      121 acatatgtta ttttattaat tttatttaaa cagataataa taaataataa tttatctcaa
      181 gttttctatg tagataaact aatctaatgt ttagaaagga ttatataata tgaataaaaa
      241 atttcgtctt tatcaagttg actcatttac aaaagaaaga ttcacaggta atccagctgg
      301 tgtaattaca aatgctgatg gcttatctga atcacaaatg caaaaaataa caagagaatt
      361 aaataattca taaactgctt ttattttcaa taaattggct aaaaatcatc aagaaaatta
      421 tgatgtacat atcagatttt ttacaccaac taatgaagtt cctatatgtg gtcatgctac
      481 tattgctgcc cattatgtta gagcagtaga aaaccaattc aataacaaaa cagtatttca
      541 aaaaactggt gctggaatat taccagttga tattataaaa aaagataatg attatgaaat
      601 aattatgaca caaggaaaaa ttgaagtatc tccaccatta caacaaacct atcaaaaaag
      661 attacttaat gcatttggta ttttaaatga agatattcgt acagattgtc ctattgccat
      721 atcatctaca ggacattcaa aagttatgat tggaataaag gaattatcaa cattaaatag
      781 tttatctcca aatttatatg aattatcgaa aataagtcaa gaaataaaat gtaatggata
      841 ttatattttt acattacata ataaagaaac tctacaagtt catggaagaa tgtttgctcc
      901 tgcaattggt atttcagaag accctgttac aggaaatgct aatggacctt taggtgcgta
      961 ccttgtacat tataaagtaa tacctatacc caacacagag tttatatttg aagcagttca
     1021 aggagaagca ataaaacgca aaggcaagat gttagtaaaa gtaattattt cagataataa
     1081 gccagttgaa gtaaaaataa ttggaaaagc tgtaattgca ttctcaacaa acatagaaat
     1141 cgaattatag attctttcta agtaaatttt tgaacttttt aatactaatt taataacaaa
     1201 ctaatttata tacaatcaaa ttaatttttt tattatttgt taatatatat ttatcattat
     1261 attatataat aatatagata gttactatat cttataatta gataaatcat aattatatat
     1321 tattgttaaa actcaattat ataattttta ataatattat tttaggaggt aaaaatgata
     1381 aacgatatta aaaataaatt caaaaattac aaaccatata taaatggata caagaatatg
     1441 aagagagcat ctgttttaat tccaataata gaaataaata acacacacta tatattattt
     1501 gaagttcgct caaaaaattt gaagcatcaa cctagcgaaa tttcttttcc aggtggtaaa
     1561 atagaatcag gtgagactcc atatgaagct gttattagag aaacttgtga ggaacttgga
     1621 acttttagtg acaatataga agtaatatca agtcttgacc tccttataac accagtaaag
     1681 tttatcatac atccatactt aggatatata aaaaatatta ataatttaaa tataaacaag
     1741 gatgaagttg accatacttt tttggttcct ataaaatatc ttttagagaa tcctccaaat
     1801 acttatagta atactgtaaa agtttttcca gatgaaaatc taccatatgg ttcaattcca
     1861 aaccaaaaag attataaatt tgaaagtgga agttatccta ttctgtttta taaatataaa
     1921 gattatgtaa tttggggaat gacagcaaaa atactagaaa actttttaaa ttttttatca
     1981 gaataacact cataataaat catcaaaact tttacataag aaatttataa aagtttctaa
     2041 atttaacaat tctaaaatat caataactat attttgtagc acttttttat tttattattg
     2101 tttgaatatt tgatggtttt caatgcaatt tcaacataaa tactatgtat tagtgtatac
     2161 tgttgatgaa ttaaagtatg ataatctatt taataaagta tgaagtaaat gattttaatg
     2221 tgtctttatg gtaaaattac taataagtaa taaaattaat taaaatatat ggaggtgctt
     2281 ataatgtttg ctgtaggatt tgttgttata attgcacttg cctttttcat tttaagtgta
     2341 ctattattta atggaaaagc cagtatgcta ctagcaggtt ataactctat gagtaagaaa
     2401 gaaaaagcac gatacgataa aaaaaaatta tgtagaagtt ctagcattgt tacttttatt
     2461 gtctctataa tgctatttat tatggcattt ttgggatata gaatagagag tggtcaaatg
     2521 gacgaaaaaa aaatggttcc cttttctata atatttataa tagtcatact ttccagtgta
     2581 ttttttaata tcctctactc taacaaaaaa tgtaaaaaat aaatagttgt aagttgagca
     2641 gttaattata aaagattgac tgctttttca tgtatattga ctactcttat taaatatttt
     2701 aacatttaaa aatctatgta aaaataatga atatagtaca aacttctaat aatgatttta
     2761 cctttgtggt tacagtccac tatatataat ttaatatgat aacatggaat tactaatgac
     2821 aactaagtaa gcttttataa aatataataa aacaaaaata gatgtacaat gaaattatat
     2881 cttatatcaa atttattttt attaatagca aaaacttaca ttagcataac atatgacaaa
     2941 ttcgcttaaa ataaaaattt ttaatataat ttgttctata gtacatctat ttcgattagt
     3001 ttacataatt ttattatttt gtacaatatt aattatttac taatatccca tttaatgaaa
     3061 aagttctagg ttcagtaatt ttgaccataa ctaatttacc aattaaatca tcatttccac
     3121 cttcgaaatt tacgagttta ttttgtctag ttctaccagc aaacttattt tcattatttt
     3181 tacttcttcc ttcaactaga acttcaacta ttctatcttt atatccatca tttatttctg
     3241 cagaaatcct atttacagct tctaacaccc tattaaatct atcatgcttt atatcttctg
     3301 gtatttgatt ttccatttta gcagcaggag ttccctgtct tttagaataa ataaatgtaa
     3361 aagctgaatc atatctaact ttttcaacca catctaaagt gtccaataaa tcttcttctg
     3421 tttctcctgg gaaaccaatc attaagtctg tagaaaaagc aatacctgga acttcctttt
     3481 tagctttttc tattatttct aaataatatt cctttgtata atgtctattc atttttttca
     3541 aaaggctact gcttccacat tgtattggta gatgtaaaaa ctcacacacc ttatcacaat
     3601 ctctaattgc gtatattact tcatcagata tatcttttgg atgagatgtc ataaatcgaa
     3661 ccctttcaat tccttctata tcattcactt ttcttaaaag ttctgagaat gaaactggat
     3721 tttcaagtgt cttaccataa gaatctacat tttgtccaag taaagtgatt tctttcgttc
     3781 catttgcaac aagctctttt atctcgttaa ttatatcttc aggagttcta cttctttctc
     3841 ttcctcttgt gtatgggact atacaatatg tgcagaaatt attgcatcca tacattatat
     3901 ttacaaatgc ttttaattca aattttctat tacttctaag accttctatt acttctccat
     3961 ccacatccca tacatccact aacattttgt cagaatttat tgattctgta agtaattgcg
     4021 gaaatttata tagattatgt gttccaaata taaggtctac atgtttatat ttttttctta
     4081 attcctctac aacatgtggt tgttgcatca tacatccaca tacagctatt ttcatatctg
     4141 gattttttcc ttttagatgt tttaattgtc ctaagttacc atacactttt agctctgcat
     4201 tttctctaac tgcacaggtg ttatatatta ttaaatcact ttcttcaacc atcattgaca
     4261 tttggtatcc catctcttct aacatcgaac ataatttttc tgaatcatgt tcattcattt
     4321 gacacccaaa ggtttgagta aacacgagtt ttctctttcc agttctgttg aagtattctt
     4381 cattttgtct gtgtatttgt tctatatatt tatcttgttc ttgtattttc tctataggta
     4441 ttgttacttg ttttcttttg ctcacgtcta ttcctcctag ttaatttctt atattacaca
     4501 taaccttaat tatatcaaat aagaaaaatc ttttaaagat attattattt ttctagttgt
     4561 gttttaatag taaatctaat gatttatata tactatattt ctaaaatata aaatcatata
     4621 attattataa taataaaaag a
//
LOCUS       DAELXX010000187         4610 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.189, whole genome shotgun sequence.
ACCESSION   DAELXX010000187 DAELXX010000000
VERSION     DAELXX010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.189"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     misc_binding    179..413
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            463..1482
                     /gene="pheS"
                     /locus_tag="KPH29_003545"
     CDS             463..1482
                     /gene="pheS"
                     /locus_tag="KPH29_003545"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBG1469106.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     gene            1499..3892
                     /locus_tag="KPH29_003546"
     CDS             1499..3892
                     /locus_tag="KPH29_003546"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBG1469107.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            3917..4507
                     /gene="zapA"
                     /locus_tag="KPH29_003547"
     CDS             3917..4507
                     /gene="zapA"
                     /locus_tag="KPH29_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBG1469108.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDVYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
BASE COUNT         1827 a          525 c          932 g         1326 t
ORIGIN      
        1 aataattgag ttaattatgc attagatgta tattatataa aaaataattg agttaattat
       61 gcattagata tatattatat aaaaaataat taaattaatt atgtattgga tatatattaa
      121 aaatagtcac ataatttgaa tggaaatgat ataatactaa aataaacaat ataatattgt
      181 aaatgcaatg aaagaggaaa gtattttgat taaacttagt aaagagataa acacctaggc
      241 tgggagtgtt ttctaagagg tcatgagaag ttccctctgg agtaacagag ctgaaatttt
      301 acagtaggct ttgacgtcaa aaacgcgtta agtttgttag aggtggtttg atgattttta
      361 aattgttaaa ctactagggt ggtaccgcga aactatagcc tttcgtccct agacagggat
      421 tgaggggctt tttttataca aaaaaacgaa agggtgatat gtgtgcaaga aaaattactt
      481 gctttacgtg aagcagcttt ggctgaaata aaagaagcac aaagcataga aagtgtagaa
      541 agtttaagag ttaagtactt aggaaaaaaa ggtgagataa ctgccatact taaagaaatg
      601 ggtaaattat ctgctgaaga aagaccagta gttggtaagg ttgccaatga ggtaagagaa
      661 aacattgaac ttagcataaa ttctaaaaaa gaagaaataa atgctattga aaaagaaaga
      721 aaattaaaag aggaagtgat agatgttact caaccaggaa aagttttaaa ggttgggaag
      781 aagcatccaa taactcaaat tatagatgaa gtaacagata tatttatcgg aatgggattc
      841 tctatagcag aagggccaga agttgagact gttgaaaaca actttgacgc attaaacgct
      901 cctaaagacc atccatcaag agatatgagc gatacattct atatcaatga tggggtatta
      961 cttagaactc aaacatctcc agttcaagta agaactatga gaagtcaaga gttaccaata
     1021 aaagtaattg caccaggtag atgttttagg tcagactcgc cagatgctac acactcacca
     1081 atgttccatc agatagaagg gcttgttgtt ggaaaagatg ttactatggc agaatttaaa
     1141 ggaactatgg atatcttcgt tgaaaaattg tttggttctg atatcaaaac taagtttaga
     1201 cctcacaact tcccatttac agaaccaagt gcagaggttg atgttacttg tttcaaatgt
     1261 ggtggtaaag gttgcccaat gtgtaaatat gaaggttgga tagaaatatt aggttcaggt
     1321 atggttcatc caaatgtgct tagaaattgt ggaatagacc cagaagttta cagtggattt
     1381 gcatttggag ttggggttga aagacttgca atgcttaaat acgaaataga tgatattaga
     1441 ttattattcg aaaatgatat gagattctta aatcaatttt aattaggagg gtatgtagat
     1501 gttagtatct ttaaaatggc ttagagacta tgttgatata gacatggatg taaaagagtt
     1561 cgctgataaa atgacaatga caggaactaa agttgaaaca atagattatt atggtgaaga
     1621 aatagaaaat atattggttg gaaagatttt agaaataaaa caacatccaa atgctgataa
     1681 gttggttgta actaaagtag atattggaga taaagttgtt caaatagtta caggagctac
     1741 aaatatatca gaaggagatt atattccagt agctgtaaat ggttctaagt tacctggagg
     1801 agttgaaatc aaacagactg atttcagagg tgaattatca gatggtatga tgtgttcagc
     1861 agctgaacta ggtatagatg aacattacat tgaggagtat aaaagaggtg gtatatatat
     1921 tttagaccac gaagattctt atgaattagg aaaagatata aaagatgttt taggattaaa
     1981 agatgcttta atagattttg aattaacttc aaacagacct gattgtaaat gcatgatggg
     2041 tatagctaga gaagcagctg caactatagg aacaaaagta aaatatcctg aaatcgaagt
     2101 aaaagaaagt gacgaagaga tagatttcaa agttgagata gataatccag atttatgtag
     2161 aagatatgtt gctagaatgg ttacagatgt aaaaatagaa ccttctccat attggatgca
     2221 aagaagactt acagaagcag gagtaagacc tataagtaac atagtcgata taacaaactt
     2281 cgtaatgtta gagcttggtc aaccacttca tgcttttgat ataaatcaag tagagactgg
     2341 aagaatagta gtaagaaatg ctaaagatgg agagaaactt gtaacattag atgatgttga
     2401 gagaacatta gataaagata tgctagttat aacaaatgga gaaaaatcac ttggtttagc
     2461 tggtgtaatg ggtggtgcta actcagaaat aacttctaat acgaagactg tactttttga
     2521 aagtgccaat ttcaaaccag aaaacataag aatgacagct aaaaaagttg gtattaggtc
     2581 agaagcatct tcaagaaatg aaaaagactt agaccctaat cttgcagaga tagcagcaaa
     2641 tagagctgca caacttgttg aaatgttagg agcaggaaaa gttttaaaag gtgttgtaga
     2701 tgtatatcca aataaaccag aacctaaaaa attggtagta aatcctcaaa gaattaacca
     2761 cctattaggt gtagatgtac caatggagca gtttgtagga attttagaat cattagagtt
     2821 taaatgtaat ttggtagcta atgataaatt agaaatagat gtaccaagct ttagaacaga
     2881 tatggaacaa gaagctgatg tatgggaaga aatagctaga atttatggat ttgagaatat
     2941 accttctgta caattagaag gaaatacgac agcaggaatt aaaacttcaa aacaaaaatt
     3001 catggatgct ttaaaagata attcaactgc tgtaggatta aatgaaatat taacatattc
     3061 atttgtaagc cctaagggag tcgataagat aagagtacca gaaggtaatg ctaagagaaa
     3121 ctttgttaaa ttacttaacc cattaggaga agaaacttct gtaatgagaa ctactttaat
     3181 accaaatatg ttagatgttt tatcaactaa tgtatctcat aaaatagaag aagtatctgc
     3241 atttgagtgt ggacatatat tcatcccaca agattcagaa ttacctaaag aagaaaatag
     3301 aatgtgtgta ggtatgtatg gtaaagatgt tgacttcttt gcactaaaag gaactataga
     3361 aactatactt gtaaatgtcg gatttaaagg ttatgaaatt gaacctcaag ataataatac
     3421 taccttccac cctggtagat gtgctaaaat agtgtataat aataaatatg taggtacatt
     3481 aggtgaattg catccagatg ttatagaaaa ctacaatcta ggtcaaagag tttatgtcgc
     3541 tgaaatagat attgactttg tatttgataa ttctgataga acaaagaatt atgtgccatt
     3601 accaaaatat ccatctacat caagagatat agctcttatt gttaaagatg atgtatttgt
     3661 aaaacaaata gaagatataa taaaggaaaa tggtcaagga ttagtagaaa gctacaagtt
     3721 atttgatgtt tacaaaggtt ctcaaataga agctggatac aagtcaattg cgtattcaat
     3781 aacttataga agtaaagata aaactctaac agatgaagat gtagctaagg tgcatgataa
     3841 gatattaagt gaattaagcg aaaaattaaa tgctaactta agatcaaatt aacaactaat
     3901 gtcgggggag gttaggatga acaaagtaat ggttaaaatc catggcgctg aatatccaat
     3961 ggttggagac aaatcagaga agtttatgat tagtattgct gattttgtag acaaagaaat
     4021 ggataaaata acacgtcaaa acccgaaatt gagcttatca gttgctgcga tactaactgc
     4081 attaaatata tcagaccttt tatttgaatg ttcagatgag aatgagaagt tgattaaagc
     4141 taatgaagaa ttgagtaaga aggttggagc atctaatgaa gagcttcaat tagaaatcaa
     4201 gtctttaaag cttacgatag cagaaaaaga agcagaaaat cgtgaagctg agactaagat
     4261 gaaagagtta attgagatta tagaaaataa aaagcaagaa atattcgaac ttagtaatac
     4321 gacagaaggt tctagagcag agttagatgt ttataaaaac aagattgagg agttaagtac
     4381 tcaacttgaa gaagcaaatg aaagagctac tatagctgaa aacttagctt ctgagtttca
     4441 aaacaaagct tatgatttac agttaaaatg tacagggtta aataatgatg caaaaaatgt
     4501 ggaataaact aaaagatttg ctgtctcttg ttttttgaga cagcttcttt taatgtataa
     4561 atataaatgc tatataaata tagctaatgt ttaaatgtaa ataaatataa
//
LOCUS       DAELXX010000188         4522 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.190, whole genome shotgun sequence.
ACCESSION   DAELXX010000188 DAELXX010000000
VERSION     DAELXX010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.190"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            177..1652
                     /locus_tag="KPH29_003548"
     CDS             177..1652
                     /locus_tag="KPH29_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBG1469109.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            1739..2152
                     /gene="speD"
                     /locus_tag="KPH29_003549"
     CDS             1739..2152
                     /gene="speD"
                     /locus_tag="KPH29_003549"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBG1469110.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            2182..3033
                     /gene="speE"
                     /locus_tag="KPH29_003550"
     CDS             2182..3033
                     /gene="speE"
                     /locus_tag="KPH29_003550"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBG1469111.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            3023..3901
                     /gene="speB"
                     /locus_tag="KPH29_003551"
     CDS             3023..3901
                     /gene="speB"
                     /locus_tag="KPH29_003551"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBG1469112.1"
                     /translation="MKNNFYHMNTFMSMDKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNMLNGKNIYLTIDLDV
                     LDASVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDKIK"
     gene            complement(4081..4281)
                     /locus_tag="KPH29_003552"
     CDS             complement(4081..4281)
                     /locus_tag="KPH29_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG1469113.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
BASE COUNT         1794 a          503 c          774 g         1451 t
ORIGIN      
        1 tattgatata gctaatgtat atgtttttga gtaacttcaa aaaaacactt gtaaaaatgc
       61 tggaatgtta atataataaa tatcatgagt ttaataaaag taaaaataaa aaatagaaat
      121 aataaataat aaatattaaa aatatttgaa tatagagagg agatgaattt gaaatcatgg
      181 atagcgagtt tgcaaatgca gttgaattaa gctatgagtg tgctccatta ttagagtcgt
      241 taaaggaata ctcagagaaa gatatagctt gttttgatgt tcctggacat gtaaaaaata
      301 gaggtgtagc tatattaaat aaatatctag gtgaaagtct catgaaaatg gatataaatt
      361 catcaccaac tatggataat gtatctgcgc caaatgggat tataaaaaat gcacaagatt
      421 tgctagcaca agcatatatg gctgatgagg cattctttat aacgaatggc acaactcaag
      481 caatacatgc aatgattttg agtgttataa aaccaggtga aaaagtatta cttccaagaa
      541 atatacataa atcagtaata aatgcgttaa tactttgtgg aggaatacca atatttatac
      601 aaccagaatt tgatgaaaaa ctaggtataa gtttaaatat tactcttgaa aaagttagaa
      661 cagagataga aaaagattgt aatataaaag cattattttt ccttaatcca acttactatg
      721 gagtttgtgc agaccttgag agtattatag aactatgcca taaaaataat gttttagtgt
      781 tagttgatga agctcatggt gcacattttc catttcattt agatttacct ccttctgcaa
      841 taagtttagg agctgacatg gtagctgtaa gtatacataa aactggagga gcattgacac
      901 aatcttcagc actcttattg aatagagata atgtaagttt tgaaaaggtt cttcaatcaa
      961 tcaatatgct acaatcaaca tcagcatctt atttgcttat ggcaagtata gatggtgcta
     1021 gagttaatct agttgagaat ggtgaaaagc aactgtcaaa agcattaaat ctatctaggt
     1081 atgcaaaatc taaattgaac aaaattgatg gaataaaagt tttatctaca gaaatattga
     1141 aacaaaaagg tgttaaattt atagatgaaa caaaattgtg tataaatgta aaagaattaa
     1201 atttgactgg atttgaagta tacgatttat tatataaaaa tttttcaatt caagtagaat
     1261 tgggagattc atataatata ctggcacttg tgtccattgg aacaaataaa tcagatatag
     1321 atagattagt aaaagcactt tctataattg caaagattta tagaaaagaa tcaacattaa
     1381 atgaatttaa tatggttcaa ataaatccaa ttattaaact taatccaaga gaagcttttt
     1441 atgcaccaaa ggaaagtgta gaaatcaatt catgtataga tagaatttgt ggagaatcaa
     1501 taatggcata tccaccaggt ataccgatta ttgctccagg agaattgata acagaagaaa
     1561 ttatggagta tattattttc ttaaaaaata gcaatgcgta tcttacagat gtacaagata
     1621 aaaacttaga tagaatacta gtaataaaat aaattacttt attttgcaag ggcaagtgtt
     1681 tgttaagtag agtttatcaa atagaactta ttaaaagtat aaaggagttg gggttgaaat
     1741 gaaatttgaa acactaggaa gacatatact agtagaatac tataattgtg atgaaaacgt
     1801 acttaaagac ccacgtctta tagaaaattt tatgaatgat tcagcattaa atgcaaaagc
     1861 gacaattgtg gattctgtat tccaccattt taatccttgg ggggtttcag gagctgttat
     1921 aattgctgaa tctcatttga caatacatac atggcctgaa tatggttatg cagcagcaga
     1981 tttttttact tgtggtgata tagacccatg gaaaagtttt gaattattag agcaactttt
     2041 aaagtctgaa agaagcgagt ctacagaaat tccaagaggc ttaactacaa aaattaaaaa
     2101 atattcaaaa aaagatttag gtaagattac tcataaacca gaagcagtat aaaacataca
     2161 agagaaagtg aggattatta tatggaactt tggtatacag aagaatggac agaaaatgta
     2221 agattttcta taaaagtaaa taaacatcta tttgaaggaa agtctcaatt tcaaaggata
     2281 gatgtatttg atagtgatga atttggaaaa tttctaacaa tagatggatt aatgatggta
     2341 acatataaag atgaatttat ataccatgag atgataactc atgttcctat ggctacaaat
     2401 ttaaacataa agaaagtatt agtcataggt ggaggtgatg gaggtacagt aagagaatta
     2461 agccgttatc ctcaaataga aaagattgac atggttgaaa tagataaaat ggttgtagat
     2521 gtatctaagg agtatatgga tatatgctca tgtaaattag atgataaaag agtgagttta
     2581 tattttgaag atggtgttaa ttttgttaag tgtgcacatg ataaatcata tgacctgata
     2641 atagtagatt caactgaccc aataggacca ggcgaagggc ttttctcaac tgatttttat
     2701 aaggattgtt atagaatact gactgatgat ggaattttag taaatcaaag tgaaagtccg
     2761 tattttgact ttaatgctaa agagatgaaa agagctaata aaaaattaaa acagatattt
     2821 ccaatatcag aagtttatca agcacatata ccaacatatc catctggtca ctggttgttt
     2881 ggatttgctt caaaaaaact taatccagta aaaaatcaag atagaaatgg atgggagaaa
     2941 ttaagtttaa aaactaaata ttataatagt gatatacatt taggttcgtt tatgttacct
     3001 caatacgtga aggagatgct agatgaagaa taatttctat catatgaaca cttttatgag
     3061 tatggacaaa aattatgaag aatctaatct tatagtattt ggtgttggat ttgatggaac
     3121 tacttctaac agacctgggg ctagatttgc aagtagctct atgagaaaag aattttacgg
     3181 tcttgagaca tatagtcctt ttttagattt ggacttagag gattataata tatgtgatta
     3241 tggagattta gaaattagtg ttggaagtac agaacaagtc ttaaaagaaa tctatcaaga
     3301 gacatataag attgttagag attcaaaggt accctttatg attggaggag agcatttagt
     3361 tacattacca gcctttaaag cagtacatga aaagtacaat gatatatatg taattcattt
     3421 tgatgcccat actgatttga gggaagaata taataatagt aaaaattctc atgcaacagt
     3481 aattaaaaga atatgggata ttgtaggtga taataaaata tttcaatttg gtataaggtc
     3541 tgggacaaaa gaagaattta aatttgctac agaagaaaaa cacacataca tggaaatagg
     3601 aggaatagat acgtttgaaa atatagttaa tatgctaaat ggaaagaata tttatctaac
     3661 tatagattta gatgtattgg atgcatctgt gtttccggga acaggtacac cagaacctgg
     3721 tggtgtaaat tatagagagt ttcaagagat ttttaagatt ataaaaaact ctaatataaa
     3781 tatagttggt tgtgatattg tagagttaag ccctgattac gatacaacag gtgtatcgac
     3841 agttatagct tgtaaaatct taagagagtt atgcttaata atatctgata aaattaaata
     3901 gagtatttta tataaatagt atatattata atataagaag ttattttaaa aaagttagtt
     3961 taagtttgac gcttttctaa agtaacttct tatttttgta attaaaaata aatataataa
     4021 acaacaaaaa agagccctcg atagctcctt ttctaaaata tatatattta aattaaataa
     4081 ttatattcta tttacgtttt cagcttgagg tcctctatta ccttgagtta tatcaaagct
     4141 aaccttttcg ccttcttcta aagttttgta tccatcattt tgtatagctg agaaatgtac
     4201 gaaaacatca tcttctccct ctacagatat gaacccaaat cctttttcat tgttaaacca
     4261 ttttactatt ccgtttttca ttataagaaa acctccgcat tattaaaaaa tacaaactaa
     4321 aaaaataagt ctgttctatt aatataacat aaattttaaa taaaagcaaa taaaattttt
     4381 aaaaaaatta attactttta gaatttaata aaaattgtta aattatacag tgagaagtat
     4441 tgtaaaattt gacgaaaatg taaatgtaga taataaattt aatgtcaaaa aatgcaacaa
     4501 aaaggtatgt aagtaaaaag ta
//
LOCUS       DAELXX010000189         4517 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.191, whole genome shotgun sequence.
ACCESSION   DAELXX010000189 DAELXX010000000
VERSION     DAELXX010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.191"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            166..1032
                     /locus_tag="KPH29_003553"
     CDS             166..1032
                     /locus_tag="KPH29_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBG1469114.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPIDK
                     ESGKPCINLQPDFKCSVHNSLMKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSPELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMSPEQIINLD
                     IISHRTKVNLLLSQASESVMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRSTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
     gene            1145..1549
                     /locus_tag="KPH29_003554"
     CDS             1145..1549
                     /locus_tag="KPH29_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HBG1469115.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEAIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            1727..2176
                     /locus_tag="KPH29_003555"
     CDS             1727..2176
                     /locus_tag="KPH29_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG1469116.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(2332..2709)
                     /locus_tag="KPH29_003556"
     CDS             complement(2332..2709)
                     /locus_tag="KPH29_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG1469117.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLDSNPNNPIMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            2971..3888
                     /locus_tag="KPH29_003557"
     CDS             2971..3888
                     /locus_tag="KPH29_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HBG1469118.1"
                     /translation="MVNMNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKC
                     MNVPIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWGTCKETIENSI
                     NAFIKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINR
                     LPVLIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGEEMLGPWVSRTDI
                     ETLNTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKY
                     YLEKTNKSIIEATLLPYSEIIETVKEFWE"
     gene            4371..>4517
                     /locus_tag="KPH29_003558"
     CDS             4371..>4517
                     /locus_tag="KPH29_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBG1469119.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGIY"
BASE COUNT         1793 a          477 c          740 g         1507 t
ORIGIN      
        1 tagtataaag gtattgttac aagtataaag aaacttatgt atgctattgg aataaataat
       61 accttaaatt ataattaaga ataagaactg ttaaataagg gacttgatta tcatttagtt
      121 atagataata taatattaaa aataaagctt aagaggagaa aaaatatgat agatatacat
      181 aaaaatatat atgataataa gttatttgag gagctaaaga ttgattgtaa gaagtgtttt
      241 ggactttgtt gtgtagctct ttatttttct gcttcagatg gatttcctat cgataaggaa
      301 tcaggaaaac cctgtattaa tttacaacca gattttaaat gttctgtaca taatagtctt
      361 atgaagcgag gttttaaagg gtgtactgca tatgattgtt ttggttcagg tcaaaaagta
      421 gcacaagtaa catataaggg gatagactgg atgcaatcac cagaattaac aaatcaaatg
      481 tctgaagtgt ttttaattat gagacaatta catgaaatgt tatggtatct taaagaagca
      541 tctgtattaa atatatcaga tacaatcaaa tcaaaaattg atttgataat tgaagaaact
      601 gaaaagataa ctaatatgag cccagaacag ataattaatt tggatatcat atcacataga
      661 acaaaggtta atttattatt atcacaagcg agtgagtctg tgatgggaaa ggttaaatca
      721 tttatcaaga cttctactct taaaaatatg aaaaaattaa gtaaaaatat agatttaata
      781 ggagcagacc ttagaaaaat aaatctaata ggagcagatt taagaggtag atttctaata
      841 gcagcaaatt taagaagtac agatttgagt ggggcaaatt taataggagc agacttaaga
      901 gattgtgata ttagaggtgc taatcttgaa aatagtatat ttttaacaca attgcaagta
      961 aatactgcaa aaggagattc tagtacgaag ttaccagctt cactaatacg ccctaaatac
     1021 tgggagaaat aattaaatta atacataatt ttacaaaata tgaacttttt tgactttaat
     1081 tcatattata cattagagat tctttacaag aatttcttaa tataatatag ggagagatat
     1141 aaaaatgagt tatcaaaatt ataaatgtga aagagattat gtgtatgatg attataaata
     1201 caataagtgc aacaagtgct ataatgatta tgaggaaatg acacatgttc atgaatattc
     1261 agaaagtgtt aagttagcag aagaatgtga ggatagacat aaccaccgtg cagcaggggt
     1321 tactggtgaa gctataccaa taaatggagg tacaaatcat gttcataaaa taaatgataa
     1381 tgtagatttc cttgaccatt tccataagat atgtgttaca acagggccag caattagaat
     1441 acctggaact gataaacaca ttcacttaat atgtggagaa actactgtta atgatggtca
     1501 ttgtcataaa ttccttttta ctactcaaat agaagcacct ttagtataaa ctgatatgga
     1561 ttattgtagt aaatttgcta gattatagta tataataaat gttaaaaata tataatagat
     1621 aatatttaaa cttaattagg actatgatac ttaaccttcg tagtcctttc taagttttgt
     1681 ataaaaaagt gttatacaat taaaagtgat atagggagga aattttatga aatctggaat
     1741 aattcttgaa atgaaacaaa taaattcaac agaatatgac gaaatgttgc aagtctggga
     1801 atcatcagta agagcaacgc atgatttttt aacagaaaaa gatattgaat ctttgatacc
     1861 attagttgaa attggactta aagaagtaga aaatatagtt tgtataaaag ataatgatat
     1921 aataaagggc tttataggaa tagataaaga taaaattgag atgctcttta tagaagataa
     1981 atatagagga aatggtattg gtaaaaaatt aataaaatat gctatagata aatacaatgt
     2041 aaaatatgtt gatgtaaatg agcagaataa aaaagctgta ggattttata ttcacttagg
     2101 atttaaagtg tttgataggt ctgaaataga tggacaaggt aacccatttc caatactaca
     2161 tatgaaatta atttaaagtt atacttctca cttattcgca aaaatattta cttaaatttt
     2221 tatacaaata aaagtcgtga cagtcttagg acacgacttt tttactacac tattaaattg
     2281 aaatttatct gttctatatt atgattatta gatgtagttt ttttattttt actatgtact
     2341 taaataccaa gtatacccat acctatcaag tacttcagca gcgccatcat tttgatgtgg
     2401 tggtgggtgt ggtaaaaatt cttgtacaag tgtaccaccc tcggcaagtt ttacagatat
     2461 ttcttctatc gcttctcgag atggtagaac aacacaaatc gccatgccag aaattacaac
     2521 aggcttgtct gtatccgcac ccattataca accttcgtat tttgtgaaag taacacttgc
     2581 gtgcataata ggattgtttg gattgctgtc taatcgcgca aatatagttt ttcctcctcc
     2641 gaaagcctca gcataaaaat taaatgcttt ttcacaagta ccattaaata ttaaatatgg
     2701 agttagcatg accaatatat cctccttgat atagaataat ttatagttgc acctgataat
     2761 ctaaattatg atttagtaaa ttgtatcata gggaaacctt gaagtatata attttttatt
     2821 attatattat acaaatttat taatttaaaa aaatgttata tgtacggaag gtttacagga
     2881 taaaatataa taaaaagatt aaggtgacat tataaaaaat gtatattgac attgacgcta
     2941 cgtaattgta tatactttgt cttaaaggag atggtaaata tgaatattat agaaattcgc
     3001 tcagataaaa tatacaagaa gataatggat gcaccaataa acaaaaaaga agatatatac
     3061 agatatgaat tgatgaagcc ttttgaattt aagtggaagt gtatgaatgt tccaatagtt
     3121 gctagacaga aaggtggata tgatgtaatt atagcaagtg aaatgttagg ggttttatcg
     3181 cctaaggata ttgatgaaaa gcaaaaaaag aatataaatg tgttatctgc tgataaaatt
     3241 tggggcactt gcaaagaaac catagaaaac tctataaatg cttttataaa agaagggtat
     3301 gatttaaaca ttaaggacta taaatattca atattattgg cgaatccaaa tagtccttat
     3361 acaatattaa gtgatggata ctggggtgat ggtgggattc ctggatatat atttctatca
     3421 ttggttccta atgaatatac tatcaataga ttaccagtat taatagcaca tgaatgtaat
     3481 cacaatatta gatttcagtt tatagagtgg aataataata taacattaga agaaatgatg
     3541 ataaatgaag gtcttgcaga aaattttgca acatggatgt ttggagagga aatgttagga
     3601 ccttgggtca gtagaacaga tatcgaaaca ttaaatactt atataaagcc aataataaaa
     3661 agtgctttaa aagaaactgg atttcaaaat ataacatctt atctttatgg tgatgatata
     3721 gctaaaatgc aaggatattt tccagtaggg ttgccttatt gtgcaggata tgcttgtgga
     3781 tattatatga ttaagtatta tttagaaaag acaaataaat caataatcga agcgacttta
     3841 ttgccttata gtgagataat cgaaacagta aaagagtttt gggaataaat tttataaatt
     3901 gcataatttg aaataaatta tgcaattatt ttatttttaa gaaaaaataa attgacaata
     3961 atttataaga aatatacaat aatatatata aagaaatatt tcaatattta aaagttttaa
     4021 gatttcatat ctagagtacc taacattaca tgttagtttc ttgttactta tctaattact
     4081 ttatatttcg tagttatata taaaattact ataaaaaatg aaatatatta aacaatcaac
     4141 taaaaaatga aatattaata aaaaaaattc aaattataaa tattaaatga atataattaa
     4201 aacaaatata taaatttaag tataacactc acattttaat gtattgtgaa atttttgttg
     4261 aactttatgt accataactt ataaaattta tgtcatgtta tatttgaata agtaataaaa
     4321 ttgtagatgc aaattaatct acaaaattta tatgaaatgg agttgatgct atgacagaat
     4381 taatgattat aagtgggtta gtactattga tttgtataac ttctagcaag gtattgtata
     4441 aatttggagt accaatatta ttgattttta tcttactagg gatgttattt gggtctgatg
     4501 gaatggtagg aatctat
//
LOCUS       DAELXX010000190         4515 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.192, whole genome shotgun sequence.
ACCESSION   DAELXX010000190 DAELXX010000000
VERSION     DAELXX010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4515
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.192"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(153..953)
                     /locus_tag="KPH29_003559"
     CDS             complement(153..953)
                     /locus_tag="KPH29_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBG1469120.1"
                     /translation="MTFKNKKFTINKYEILSIILVLCLILTGFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNVGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPAILHTPNL"
     gene            complement(1268..1999)
                     /locus_tag="KPH29_003560"
     CDS             complement(1268..1999)
                     /locus_tag="KPH29_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1469121.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNPHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEVCAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
     gene            complement(1986..2681)
                     /locus_tag="KPH29_003561"
     CDS             complement(1986..2681)
                     /locus_tag="KPH29_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG1469122.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMNVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            complement(2683..3474)
                     /locus_tag="KPH29_003562"
     CDS             complement(2683..3474)
                     /locus_tag="KPH29_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1469123.1"
                     /translation="MKNKFFTTLLTGFFAIVFFFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAITLSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQVFVNLPLTIKILHTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFISLTCLMLFEIFFNREVDKN"
     gene            complement(3484..4296)
                     /gene="modA"
                     /locus_tag="KPH29_003563"
     CDS             complement(3484..4296)
                     /gene="modA"
                     /locus_tag="KPH29_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG1469124.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
BASE COUNT         1547 a          643 c          555 g         1770 t
ORIGIN      
        1 taatgatgat atctttatat attttaagag atgatgaaat tagatatggg taatagttga
       61 atgagaaaaa gtagtatagc acaaaaagtg ctatatcaca aaatctcaaa taatttcctt
      121 cttataactt aaattaaaag aataaatatt tcttataaat taggtgtatg aagtatagca
      181 ggaacatctt tattatttaa taacttatca aatgatttat ggtcagactt atagccaata
      241 ccttctctat attctaattc atcaacaatt ttgtattgtt gacaggcatt tttaaccttt
      301 tcatagtctt cagtagaatg agctttataa actatcttat tattaaaagt tattttatca
      361 gcaaatttag gttttacaaa atatttagca tcttttattt taccaacatt atataaattt
      421 tggataacag aaccattttt taaatgttgt aaatggtcat ctaacttata agtatgtgct
      481 atatgagtta catttttaaa tttttcttca aatgtaagag caattttttc catgtaattg
      541 aagtcagtat taccatttgt atttgtttct ggtaataata ttatattttc ccttattaca
      601 ccaaggctat taagagaagc taaaaattct ctatttctat attctgattt ttgtatattt
      661 gttaggtttt catatttttt atatttatca aagacggata taccacatcc agtagatact
      721 aaaactacaa atacatgatt ttttcctctt tcttttatag cattaacaat agcactccca
      781 gcccacaaca cttcatcatc ttgatgctga acatagaaga caacataatc tttaaatttt
      841 ttatgactat aattcacata gctactaaaa ccggttaaaa tcaagcataa aactagaata
      901 atagatagaa tttcatattt atttatagta aattttttat tcttaaatgt catttaatca
      961 tctcctgaaa agttgtctta gttatattat caaacaaagt actaaaaatt atttcttaaa
     1021 tgctgaaaaa tatagagtag attaatggaa taaaattttt tttattagtt taaaataaag
     1081 aaaacattta tgtataagac taatcaaaat attaataatt gataggaatt gagatttaaa
     1141 gggattgtac atttattcgg gattcatttt aatagaattt tactttagta cttagaataa
     1201 aagtaataac tttttaaatt taaagagtac acatatatat ttgtgtactc cttgatgata
     1261 tgattgctta tttactacaa aaacagagtc tttttaaacc taaaatattt gatgcaccag
     1321 ctatcgtagt tccataatag taaactggtt tgttaagtga tagaaaattt ataattgagt
     1381 tattacataa tgtacttcct gtgcaaatca ctaaatcagc ccaagatatt acatcttcat
     1441 aatctcgtat accatcttca atcttaacat tatattgaat agtatccaca aattcaggat
     1501 ttaaatctaa tactcttgtt tcgaaaaagt ccttaatatt atcaattatg gctggttgat
     1561 atcctataat tgctactttt ggattaccaa attccatttt tataaactca gggaattttt
     1621 ttgcacatac ctctggctcg ttatttttac aatgaatggt tctatccgtc ttgcctaagt
     1681 atttcataac tgcatttaat gatgctataa aaagacttct atcatgagga ttgtcatgta
     1741 aatctaatga taatatgtct tttaaagaac cttcaaatgt agatggagta gaagtaaatg
     1801 cttgtccttt agctccttta aagtcagctt caagcattat ttctttacca actataattg
     1861 ggaaatcttt acgcttagta tttccaattg cttcttttga agatagagat ttggacacaa
     1921 tacttatcat atccatattt aaattatatt tatcgacttg tactaataga taatctctca
     1981 aaatatcaaa tatattcata gaaaaaagat tctcctttat tttttatttc ttcatttaaa
     2041 tcaaaaatat gatttatttt tccattgttc attattccta tcttatgagc aaaaaacatt
     2101 gcttcagaaa tatcatgagt aacatgaatc actgttggtt tgtattttat gtagacatcc
     2161 ttcaaaattt tgcttatatg tttttttgta atataatcca atgaactcat aggttcatct
     2221 aaaagtaata tatcaggttc agctattaag gcacgtgcaa aagcaactcg ttgcttttcg
     2281 cctccactta aatttttagg atacctattt tttatatctt ctatctgcat taatttagac
     2341 aagtggttag ccttaagaat ctgttcatct ataggtattt ttcttaattt aagtggaaac
     2401 cttatattat cttcaacatt catatttgga aataattcaa aattttgata tacaaatcct
     2461 atatttctat tttctggagg taagtttgat attaaatttt ctttgtatga tacatttcct
     2521 ttagcagatg gatatcttcc tgctaatgtt tctaggaata gagttttacc tgacccacta
     2581 gctcctaata aaacaaagta ttcatgatta ttaatattta aactattttc ttgtaattta
     2641 aaattaggta aatcaagaga taaatttttt atttttaaca tattaattct tatcaacctc
     2701 tctattaaaa aatatctcaa aaagcatcaa gcatgtaagt gagataaata tcaaaattac
     2761 agctattccg attgcaatgt ctatatcacc agtagacata tttaaatata tagatgtagg
     2821 aataatttca gttttcatac gtgtagaacc tgctatcata gctactgcac caaattcacc
     2881 aagtgctctt gaccaagtca ttacagttgc agatatgatt ccagttttta gatttggaag
     2941 aattataaat ctaaatgctt cccaactatt acatccaagg gtacgtgcaa caaatatcat
     3001 cttttcatta ctttcattta gtgaagtgtg aagtatttta atagtaagtg gaagatttac
     3061 aaatacttgt gctaaaacta taccttgctt tgtaaatata aaatcaagct taaaaatctt
     3121 ataaatggaa tctccaatcc ccattcttcc aaacaaaagt aaaagagcaa ttcctgatac
     3181 taagtgtgga agagacatag ggagatttat tatctgtata atcagttttt taaatggtaa
     3241 tttagtaata tgtaaaaaat atgctactgg aatagctaat aaaaggcata ctatagtaga
     3301 tattaaagat gttttaatac taagtgttat tgcatactgt acttccacgg attttaagca
     3361 tataggaaca taacttatcc ctttaatgat taacataaga agtggtgata caataaaaaa
     3421 aaagactata gcaaaaaatc cagtaagtag tgtagtaaaa aatttatttt tcaagtttaa
     3481 tcactactta acaggttcaa aaccatattt tttaaatatc tgtttagctt catctgactc
     3541 tatataattt ataaatttat tagctaaatc ttttttatca ctatagttta cgattcctgc
     3601 tgatatgagt tggtctatat ttttatcttg ggcaatttct ataacttcta catcttcatt
     3661 gccaaagacc gaatcttttg tggctattgt agcatcagct ttacttgatg ttaactgtac
     3721 aatcatttca tttacagttg cagctgtaga gacaacatta tcatttatag attttaaatt
     3781 atttttttca agtatttttt gtgtagtctt acctatagca ttagactctt tgtctcccaa
     3841 aataagttta actccatctt tatttaagtc ttccaatttt tgaatattag ctggatttcc
     3901 tttaggtaca actattgcag gtgtatgatg tgcaaccgtt ttatgttcac ctacaagttc
     3961 tttttcaaag gcctttgaat aaattggttc agaaccaaca ataaatacgt ctcctttgtg
     4021 acttgtctca atttgagcta ttagctgtgc agaacctgca tagctatatt caactttgac
     4081 attattttct ttctcaaatt tttttgctat ttcatccatt ggtttcttta aacctgcccc
     4141 tgaatagaca aaaagagtct ctttttcttt tgaatcatca gctgttgtca ctttagtatt
     4201 agattcatca ttagctttgt tttctttaga attacatgca gtaatactta atgaaataat
     4261 catagctaaa gctagtactg aaagtttttt aaacataaga tattccccct tagtaaatta
     4321 gtgtaaattc ttttttagta tatcatacta agaaaacgat gtctaagttt ttgttatgtt
     4381 ttataatgtt ttgttatgtt ttattatgta tagttaagaa aaaatctatt tataatgtca
     4441 tttccatagg tagttatttt atttttaatt tttattaata ctttaaagta ttttaaattt
     4501 aaaaacttaa atcaa
//
LOCUS       DAELXX010000191         4475 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.193, whole genome shotgun sequence.
ACCESSION   DAELXX010000191 DAELXX010000000
VERSION     DAELXX010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.193"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..410
                     /locus_tag="KPH29_003564"
     CDS             <1..410
                     /locus_tag="KPH29_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HBG1469125.1"
                     /translation="FNLLSNCLDSKGAKVIHSVEELLEFKNNRLKDIVEFYAVYIDDI
                     MICGSMVFNFQERVYHTQYLASNSTYNKYYPMIFLYYNLIKDALERNFKYLSFGISTL
                     NRGKYLDEGLATFKEGFGSSAICNRTYYKNINK"
     gene            502..1734
                     /locus_tag="KPH29_003565"
     CDS             502..1734
                     /locus_tag="KPH29_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HBG1469126.1"
                     /translation="MKRLMILGSIKYFENIVMSAKKEGIYTIVCDNRIDTPAKKICDE
                     AIDVDVFDFDKIKSIAIDKKIDGILTGFTDSLMKPYVYIANELNLPCVISCNQLESVT
                     NKAIMKKYFKNNAIPTTDYCIIESLKDLEKIQGLNFPLVIKPVDGYGSRGVYFVSNIE
                     ELIEKFEYSKEASQDGQVIIEEFYESEEIQGLAWVHNGESHVFYIGDRELVNIHTGRA
                     GKPDRLLYPSKYCFQYEEEIKSIYQNITQAFDIKNGPLYVQMLVGAYGIKVSEVMPRL
                     PGGCDYMAISQITDLDIGSLFANFSVNNEVNFDEIKKYNMHLSKCVYALPIYIRPGTI
                     KKINNIDKIENLDYVTQFLLLVKEGDTIGLYEDERQDCGRVYGIANNIFDANIKKKCI
                     HEMIEIFDENNENMIENF"
     gene            1754..2698
                     /locus_tag="KPH29_003566"
     CDS             1754..2698
                     /locus_tag="KPH29_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HBG1469127.1"
                     /translation="MKLITHEQIMSLNISQKQGYEWIKESFLTKKDCQLPPKISMVEE
                     GHVFYNVMPSLNHNHMIGGIKMVTRLPGRNPSLNSDIFLYNMKDAKLKAILDGNYITA
                     FRTAAVAGLAIDTFAKKDFNTISIMGLGVTASAIMNMLSYTLKDKNINIKLLKYKDQA
                     ESFIKKYEKNEQFSFEICDTHEELIVDSDVVISTVTYTDEYLSEFKWFKKGALLLPVH
                     VMGFGNVDYLFDKVYLDDYNHLKHLENIDRYKYSCEFYNVLSKQAKGRENDDERIISY
                     NIGLSIHDILFANKIYELCETLNCEEIELKEPKEKYWI"
     gene            2792..3199
                     /locus_tag="KPH29_003567"
     CDS             2792..3199
                     /locus_tag="KPH29_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HBG1469128.1"
                     /translation="MDKYNLIKFLSIGNEYIGNLVALESNKAIPFDIKRVYYIYDVPN
                     NVKRGFHAHKNLEQVLVCLNGSVKIKCYDGVKESIFNLHKNDVGLYIGTGVWHEMFDF
                     DEKTVLISIASEHYNEEDYIRDYKEFLKYLNNM"
     gene            3286..4377
                     /locus_tag="KPH29_003568"
     CDS             3286..4377
                     /locus_tag="KPH29_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HBG1469129.1"
                     /translation="MLVKFNNLLKQFQTHKEEYEQKIKDVMESGYYILGNEVRHFEEE
                     FASYVGSKYCVGLASGLDALIIAFRALGISEGDEVIVQSNTYIASVMGISINGATPVF
                     VEPNEYYNIDSSKIEEKINSKTKAILVVHLYGQASKMKDIQELCDKYNLYLIEDCAQS
                     HGAKYDGKMTGTFGDIGCFSFYPSKNLGAFGDAGAIVTSNEEIADKVKILRNYGSEKR
                     YYNKFVGYNSRLDEIQAGLLRVKLKYLNEIEIEKSKIVDRYLNEINNPKVILPKIENN
                     CTHVWHLFVLQVEKRDTFMEYLKKNGIESIIHYPIPPHLSEAYAYLGYKKGSFNIAEN
                     YSDKVVSIPLFVGMEDEEVSYIIKIINEY"
BASE COUNT         1821 a          395 c          761 g         1498 t
ORIGIN      
        1 tttttaattt actttctaat tgtttagata gtaaaggagc taaagtaata cattcagtag
       61 aagaactttt ggagtttaaa aataatagat taaaagatat tgtagaattt tatgcagtat
      121 atattgatga cattatgatt tgtgggagca tggtatttaa ctttcaagaa agagtttatc
      181 atactcaata tctagcatca aattcgacat ataataaata ttatccaatg atatttttat
      241 actataatct aataaaagat gctttagaga gaaattttaa gtatctttct tttggaatta
      301 gcactctaaa tagaggcaaa tacttagacg agggtttagc tacatttaaa gaaggttttg
      361 gttcaagtgc aatttgcaac agaacatact ataaaaatat aaataagtga taatcatgtt
      421 ttatagagtt tttagagtat agtaaatatc tagtactcta atattacata aaaataatta
      481 gtttataaag aaggtttgta tatgaaaaga ttaatgattt taggctcaat taagtatttt
      541 gagaatattg ttatgtcagc taaaaaagag ggaatataca caattgtttg tgataataga
      601 attgatactc ctgcgaaaaa gatttgtgat gaagcaatag atgttgatgt atttgatttt
      661 gataaaataa aaagtatagc tatagataaa aagatagatg gaattttaac tggatttaca
      721 gattctttaa tgaaaccata tgtttatatt gcaaatgaac taaatttacc atgtgtgatt
      781 tcttgcaatc aacttgaatc tgtaactaat aaggctatta tgaaaaaata ttttaaaaat
      841 aatgcaatac ctacaactga ttattgtatt attgaaagtc taaaagattt agaaaaaata
      901 caaggattaa attttccact tgtaataaaa cctgttgatg gttatggttc aagaggtgta
      961 tattttgtat caaatataga agaattaata gaaaaatttg aatattctaa ggaagcctca
     1021 caagatggac aagtaataat tgaggaattt tatgaaagtg aggaaataca aggactagct
     1081 tgggttcata atggagagtc acatgtattt tatattggag atagagagct tgtaaatata
     1141 catacaggaa gagctggaaa accagataga ttactttatc catcaaagta ttgttttcaa
     1201 tatgaggaag aaataaaatc tatatatcaa aatataacac aggcttttga tataaaaaat
     1261 ggacctcttt atgtacaaat gttagtggga gcatatggaa taaaggtatc tgaagtaatg
     1321 ccaagattac ctggaggttg tgactatatg gctatttctc aaataacaga tttagacata
     1381 ggaagtttat ttgcaaactt tagtgtgaat aatgaagtaa attttgatga aataaagaaa
     1441 tataatatgc atttatcaaa atgtgtatat gcattaccaa tatacataag acctggaaca
     1501 ataaaaaaga taaataatat agataaaata gaaaacttag attatgtcac tcaatttcta
     1561 ttattagtaa aagaaggaga tacaatagga ttatatgagg atgagagaca ggattgtgga
     1621 agagtttatg gaatagcaaa taatatattt gatgcaaata taaagaagaa atgtattcat
     1681 gaaatgatag aaatatttga tgaaaataat gaaaatatga tagaaaattt ttaaataagg
     1741 gagtatgata gatatgaagc taataactca tgaacaaatt atgtcattaa acatttctca
     1801 gaaacaagga tatgaatgga taaaagaatc ttttttaaca aaaaaagatt gtcaattacc
     1861 accaaaaata agcatggtag aagaaggtca tgtattttac aatgtaatgc cgtccttaaa
     1921 tcataaccat atgattggtg gaataaaaat ggttacaaga ttgccaggga gaaatccaag
     1981 tttaaattca gatatatttc tatacaatat gaaagatgct aaattaaagg ctattttgga
     2041 tggtaattat attacagctt ttagaactgc tgctgttgct ggtttggcta ttgatacttt
     2101 tgctaaaaaa gattttaata ctatttctat tatgggttta ggtgtaactg cttctgctat
     2161 aatgaatatg ctttcgtata ctcttaaaga taaaaatata aatataaagt tacttaaata
     2221 taaagaccaa gcagagtcat ttataaaaaa gtatgaaaaa aatgaacaat ttagttttga
     2281 aatatgtgat acacatgaag aacttattgt agattcagat gtagttattt ctactgtgac
     2341 atatacagat gaatatttat cagaatttaa atggtttaaa aaaggtgctt tattattacc
     2401 tgtacatgtt atgggatttg gaaatgttga ttatctattt gataaagtat atttagatga
     2461 ttataatcat ttgaaacatc tagaaaatat agatagatat aaatattctt gtgaatttta
     2521 taatgtttta agtaaacaag ctaaaggaag agaaaatgat gatgagagaa taatatctta
     2581 caatataggt ttgtcaatac atgatatatt atttgcaaac aaaatttacg aattgtgtga
     2641 aacactaaat tgtgaagaga tagaattaaa agaaccaaaa gaaaaatatt ggatatagtt
     2701 tatttcattc atatttacaa taatttattg taaatatgaa tatgaagtaa tatattgtta
     2761 gaaaattagc atgaatagga tagataaatt tatggataaa tataatttaa ttaagttctt
     2821 atctattgga aacgaatata taggaaattt agttgcatta gaatcaaata aagcaatacc
     2881 ttttgatata aagagagttt attacatata tgatgtacca aacaatgtaa agagaggatt
     2941 tcatgcacac aaaaacttgg aacaagtact agtttgccta aatggaagtg tgaagataaa
     3001 atgttatgat ggtgtaaaag agagtatatt taatttacat aaaaatgatg ttggattgta
     3061 tataggaacg ggagtgtggc atgagatgtt tgactttgat gaaaaaactg ttttaatttc
     3121 tatagcatct gaacattaca atgaagaaga ttatattaga gactataaag aatttttaaa
     3181 gtatttaaat aatatgtagt tattatacag ttagtgatgt attttcttaa ttaaataaat
     3241 ttaagatagc catatagaga ataaatagat taggatgtga atttattgtt ggttaaattt
     3301 aataatttat taaaacagtt ccaaactcat aaagaagaat atgaacagaa gattaaggat
     3361 gtaatggaaa gtggatatta tatccttgga aatgaagtaa ggcattttga agaagaattt
     3421 gctagctatg taggtagcaa atattgtgtt ggtttagcca gtggtttaga tgcacttata
     3481 attgctttta gagcattagg aataagtgaa ggtgatgaag taatcgtgca atcaaataca
     3541 tatattgcta gtgttatggg aatcagcata aatggagcaa caccagtatt tgtagaacca
     3601 aatgaatatt ataatataga ttcatctaag atagaagaaa agataaatag caaaacaaaa
     3661 gctatattag tagttcattt atatggtcaa gcaagtaaaa tgaaagatat acaagaatta
     3721 tgtgataagt ataacttgta tttaattgaa gattgtgctc aatctcatgg agctaaatat
     3781 gatggaaaaa tgacaggaac atttggtgat ataggttgtt ttagttttta tccaagtaaa
     3841 aatttgggtg catttggaga tgcaggagct atagtaacta gtaatgagga aatagctgat
     3901 aaagtgaaaa ttcttagaaa ctatggttca gaaaagcgat attataataa atttgtcgga
     3961 tataattcaa gattggacga aattcaggca ggtttattaa gagtaaaact taaatatcta
     4021 aatgaaattg aaattgaaaa atcaaaaatc gtagatagat atcttaatga aattaataat
     4081 cccaaagtca tacttccaaa gatagaaaat aattgtactc atgtatggca cttatttgtt
     4141 ttacaagttg aaaaacgaga tacatttatg gaatacttaa agaaaaatgg tatagagtct
     4201 ataatacatt atccgatacc accacatcta tctgaagcat atgcttattt gggatataaa
     4261 aaggggagtt ttaatatagc tgagaactat tcagataaag ttgttagtat acctttattt
     4321 gtcggtatgg aagatgaaga agttagttat ataattaaaa ttataaatga atattaaact
     4381 aatcataata atatcaaaaa taaattttct atatatcatt tatttaaatt tcgttaattg
     4441 acatttttaa atattgatat attgtacaaa taaaa
//
LOCUS       DAELXX010000192         4471 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.194, whole genome shotgun sequence.
ACCESSION   DAELXX010000192 DAELXX010000000
VERSION     DAELXX010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.194"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(89..634)
                     /locus_tag="KPH29_003569"
     CDS             complement(89..634)
                     /locus_tag="KPH29_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBG1469130.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKKAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            complement(752..2575)
                     /locus_tag="KPH29_003570"
     CDS             complement(752..2575)
                     /locus_tag="KPH29_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1469131.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVKEMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRLLNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGVLASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KAGTSNAIIGETGSGKTTIINLITNFYHIDEGSIFLDGKDIYSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANAHKFIVKMREGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(2588..4363)
                     /locus_tag="KPH29_003571"
     CDS             complement(2588..4363)
                     /locus_tag="KPH29_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1469132.1"
                     /translation="MKYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLDYILSTGAIMIGITFLGAIFAIIRSVISARVSQHFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSF
                     EELNDELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIEREESLDVGEKDLKFV
                     NCDIIFENVDFSYNKNTDEKILKNVSFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGRSIYSISEESLREKIGVVFQNSSVFGKSILENIVMGNEGISREDVIKSC
                     EIANANQFITKLENSYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFALQNLVSEDVN"
BASE COUNT         1456 a          798 c          395 g         1822 t
ORIGIN      
        1 aatacaacta ttaaaaagga gcatatcatt ataattttaa aaatcataaa tgatatactc
       61 ctaaaaatct acttaactta tattaaattt attttccaaa atatctattt aataatccta
      121 agaatgcttt accatgtcta gcttcatctt tacacatttc atgtactgta tcgtgtatag
      181 catctagtcc taattcttta gcttttttag ctaattttaa ttttccatca gttgcaccat
      241 actcagcatc aactctaact cttaagtttt cttttgtatc tgcaactaca acttctccaa
      301 gaagttctgc aaatttagca gcatgttcag cttcttcaag agctattctc ttataagctt
      361 ctcctacttc tggataacct tctctatcag cttgtctact cattgctaaa tacattccta
      421 cttctgtaca ctcaccaaca aagttagctc ttaatccttc gattatttct tcatctacac
      481 cttgagctac tcctattctg tgctcatcag cccaattttt ttcgcctttc atttcttcaa
      541 atttatcagc tccaacttta catactggac attgtgctgg tgcagcatct ccttcatgta
      601 tatatccaca tactgtacaa acaaatttct tcataattac tttcctccta aattttatat
      661 atcaaattaa attttttatt tattaagtta gtctatatat acccgcacca tataccttta
      721 aacaattttt ttaaaagttt tttctaaata cttattttcc ctgtgtcaat aatatgtttc
      781 tataataatg tccctttaat tttatcaatt ctttatgatt tcctgattcc acaattctac
      841 cattttctaa aactattata ttatcacaat ttgatatggt acttaatcta tgagctataa
      901 ttatacttgt ctttccataa gttaaattat ttattgcttc ttgaatttta gcttctgttg
      961 ctatatctat gttacttgta gcctcatcca aaataagtat tggagcattt tttaatactg
     1021 ctctagcaat acttattaat tgtttttgac cttcacttaa atcttcaccc ctatccgata
     1081 atacagtact atatccttca cgcattttaa ctataaattt atgtgcattt gctattttac
     1141 tagcatatat aacttcttca tcagtagcct ccagatttcc ataccttata ttttccataa
     1201 ctgtatcatt gaatatataa ccttcttgtg gaactactcc aaaacaagtt ctcaaatagt
     1261 ttctgttaat agagtatata tccttaccat ctaaaaatat acttccttca tctatatgat
     1321 aaaaatttgt aattagattt ataatcgttg tttttccact tcctgtctct cctatgatgg
     1381 catttgatgt gccagctttg atttttaggt ttatattatc taaaattttc ttttttgaat
     1441 cataagaaaa acttacatct ttaagctcaa tttctccctt tacaaaatga gagtttaaat
     1501 taccatcttt attatcgtta atctcacttt taaacatgtt atgcacttct tgctcttctt
     1561 gttcatctat aatttcaaaa actctttcac atccagctat ggctgaaagt aatgtattaa
     1621 atagatttgc taattcattt aaaggtcttg tgaattgtct tacataactt aaaaaactag
     1681 ctataacccc tatggttata atatctttag atgcaagaac acctcctata atacatatag
     1741 ttgcaaatcc tatattacta atcacattca taataggcat taaaagagaa gtgtaaattt
     1801 gcgatttaag ggaaatattt agaagtctat cattgacttc tttaaaatca tttacagatt
     1861 ttctttcata attaaaagat tttactacat ttatgtttga tatcatttct tcaatatgtg
     1921 catttagttt tcccaactca acttgttgtt gcttaaaaaa aactctagtt ctctttgcaa
     1981 taaaattact cacaaacatt actatcggta ttaatattac actgcaaaat gttaaaattg
     2041 ggcttaatac aaacatcata ataaaagtca tcaatatgtt taagattcct gtaattaact
     2101 gtactaatga atttgatatt cctgtactga tattatcaac atcatttgtc actctactca
     2161 taatatctcc agtagaactt ttatcaaaaa aggttattgg tagattttta aattttaaaa
     2221 ataaattact tctcatttct ttaactatac tttgagataa tctagctgta catatcccct
     2281 tagacagttt aactgaaaac tcaattatat aaaatgaaag taaaataatt attcctttta
     2341 agaaaatgga ttttgaataa gtatcgatat ctattatgtc aactaccttt ccaatagtat
     2401 atggtattac agttgatata attgaatcaa ttattactag caaaaaaata aatctagtaa
     2461 tcttcttatc ttttttaaag tacataaaaa ttcttttaaa cgttttttta aaatctttag
     2521 gcttttcttt tttctttaat ctactgacct tttgatttcc agtacttaag ttcaatatac
     2581 ttcctcctta atttacatct tcacttacta aattctgcaa cgcaaaaatt tttttataaa
     2641 ttggagaact aaccagtaaa tcttcatgtg tagcaaatcc aaccacctca ccctcatcca
     2701 agactaaaat cttatcacag tcaattatag aactaatctt ttgtgaaata gttattacag
     2761 tacatttctt actttccttt tctcttaaat tacttatata ctcttttata ttctttctta
     2821 taattgtctc tgtattaata tccattgcac taaaacaatc atcaagaatt aatatttgtg
     2881 gttttttggc tatagctcta gctatagtaa ttctttgtct ctgtcctcct gataaatttt
     2941 tacccctttg aaataaaacc tcatcataag aattttcgag tttagttata aattgatttg
     3001 catttgctat ctcacatgat tttataacat cttctctact tataccctca tttcccataa
     3061 ctatattttc tagtatactt tttccaaaca cactgctatt ttgaaatact acacctattt
     3121 tttcccttaa actttcttca gaaatactgt atatactacg tcctccaatc ttaatatcac
     3181 ctttatcaat tatatataat ttttgaatta aatttactaa agaagtcttt cctgaaccag
     3241 tttgaccaat cacaccaata ttttgacctt cttctatttt aaaggatacg ttttttaata
     3301 ttttttcatc tgtatttttg ttatacgaaa aatctacatt ttcaaaaatt atatcacaat
     3361 ttacaaactt taaatctttt tctccaacat ctaaactttc ttctctctca attacttctt
     3421 cattctcatc aaccaaaacc tcacttattc tctcataaga tgcttttgcc ctaacaaaaa
     3481 cattaaaaac atgtgaaatc attataagag atgttaatat tctagtcata tagtttacat
     3541 aagcaactat aatcccaatt ttaatttctc ctgaataaat taaatgcctt ccaatcagta
     3601 atattattat tatggaaaca ttaatgatta ttgtaattgt cggtctaaaa aatgacatca
     3661 tagttgtagc ttttctacta tttttaaaaa gttcatcatt taactcttca aagctttggt
     3721 tctcataatc gtatctacaa aatagtttaa ctacttttat tccacttaaa aactgtctta
     3781 attttgaatt tactttatct gtactttctt gtacattttt aaataatcta taccctatct
     3841 tcatattaag caatattata ataaatataa ttaaaactgc aattagaata attattgaca
     3901 tctttaaatt taattttata gctataatta aacttcctat acacattatt ggtgacttta
     3961 taaaaactct catcattcca ttaacgaatt gttgcatttg attgatatca ttagtaattc
     4021 ttgttattaa cgaagctcta ccaaatttat ctatttgttt taatgagtat gtatttatct
     4081 tttcaaatac atcctgtctt aaatcataag aaaaatgctg agaaactctt gccgagatta
     4141 cacttcttat tattgcaaat attgcaccta aaaaagttat acctatcatt atagctcctg
     4201 ttgatagtat gtagtctagg ttcttatttg aaacccctat atctattatt tttgccatta
     4261 tagttggttg agctaaatca caaaaagctt ccaatgttaa aaaaaatact gctgttagaa
     4321 ataatactat atattttttt atgtattttt taaaatactt cattcaatca tcccttcata
     4381 gaattttctc attaccaaaa gtatatcata atggctaatt taattgatta tatcaattat
     4441 aaatatttga tttattgtta atttttatac c
//
LOCUS       DAELXX010000193         4428 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.195, whole genome shotgun sequence.
ACCESSION   DAELXX010000193 DAELXX010000000
VERSION     DAELXX010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.195"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            211..549
                     /locus_tag="KPH29_003572"
     CDS             211..549
                     /locus_tag="KPH29_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1469133.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
     gene            571..888
                     /locus_tag="KPH29_003573"
     CDS             571..888
                     /locus_tag="KPH29_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1469134.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            1038..1715
                     /locus_tag="KPH29_003574"
     CDS             1038..1715
                     /locus_tag="KPH29_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1469135.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(2043..2810)
                     /locus_tag="KPH29_003575"
     CDS             complement(2043..2810)
                     /locus_tag="KPH29_003575"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBG1469136.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKVAVMPQNERDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            3616..4248
                     /locus_tag="KPH29_003576"
     CDS             3616..4248
                     /locus_tag="KPH29_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBG1469137.1"
                     /translation="MTIPNSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVSPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
BASE COUNT         1791 a          543 c          537 g         1557 t
ORIGIN      
        1 gtagtatggc tatgaaatta tgaaaatatt tctctaatta aaaatatgat ttatccatat
       61 ttgtttattg tagatacaga ctatacaaaa ataaatagca cttatttcag tataaaaaca
      121 tatatagtct atatttagac taaaaaagta taaatagtct atataaaaca acttaaacta
      181 tgaatataat ctaaagtgga gggataataa ttggcaaata taggaaaaat aataggagat
      241 agaataaaag tctatagaac aaaattggga tatagtcaag agtttctagc agaaaaggca
      301 ggtttacatc caacatatat tggtcagatt gaaagagctg agacaaatac atctatcaat
      361 attataatga aaattgctac tgctttagac ataccacttg aattgttatt tgcaaatatt
      421 attacaacag aaggcgttga taactctgta cctttagaat gttatgaaat gattaataaa
      481 ttaactgaaa aagaacaaat tgcaatgcta gagttaataa agtgcatcat taaatacaaa
      541 gaaatataag tgagggaaat agtaacaata atgaattata taggtaaaag acttaaagaa
      601 gaacgaaaaa aagcaaattt aacttcaaaa gaatttgcaa atatggttgg agtatcgcct
      661 tggtatataa ctcaaatcga aagtggtaaa aagaatccaa gtcttaaaac attcataaaa
      721 tttgtaaata tattaaatat ttctgcagat gttcttataa aagatattac ttccactgga
      781 aaaacatatt tagaaaatga tataaatgaa gagttaaaag acttaaattc aagagaacta
      841 aatctgatat cacagattat agacatcata aaaaagaata aaatataatt ctttatattt
      901 tatataaaaa aaaaggttat ttgaaataaa taaccttttt ttgtcttttt gtgttaacat
      961 tgtcttatag tgctaatgag tactattaga ccatgttcta tggccttatt ttaatataaa
     1021 acgattggag ataggctatg attgtgattg aaggtagtga taaatttaag attgcaaaag
     1081 aatatattga tgtagaatat actcttttta gcaaagtaac ttttaggtat gaaaagttga
     1141 aatttaaaga taatgctgaa ttggaaaaaa ttaagatgtt taaatataaa aatggctaca
     1201 tccctaataa attaaacctt tcttttggat atggattctc ttcttataaa aagcaaataa
     1261 ttagagaaac tgtagatact ttaagattga cagaaatttt ttcaagcgag aacatagaag
     1321 atattaaatt tataaaagat ggtacaaaaa aattagaaat tagcatagag aaagttgtga
     1381 aatttaaacg tcgaaaaaaa agaaattatg tttgttgcta ttgccctgat atgtatagag
     1441 atataaaact cgacaaagaa tctatcaata agatatacaa cagaaaaata aaaatagaaa
     1501 gagaagttaa tatttttgaa gatgaggatg ttataataaa caagaaagta ttgaagtttc
     1561 caaagtcttg gacaaagaat atgcaaaaat attggttaag tgaaaataag tatcccatac
     1621 attctactgt aattgatgat gatagatata aatgttgtaa tgtacaatat acaaaaaata
     1681 gagtgataat attatattac atatataacc attaatatac ataattacaa aaaaataatg
     1741 acaaatatac ataatacaaa tatatcttct actttagata aaacagttat tatgaaaact
     1801 tactatctat aaataataga aatgctagta taccatctat aatctaatgt ctagtaggtt
     1861 ttaatggcat attttaaata tgccatagtt gatttataag attttctata tcactttcaa
     1921 aatacacact aaatagttgc ttttcagata taattgccca atttaaattt tactatctga
     1981 aaagtttttt atatttaatt tatatcttca aaaataccgt ctaatatttc tatataatta
     2041 atttaattaa tactcttatg taatagatta agtactgaat ttaactcttt aaatgatatt
     2101 tgccctggtg ctgaaacact ttttgcagct ccaaaagtca atgcagaacc aaatatttca
     2161 ccacacaatc tacttataac acccattcct gacattgaca ttgttattat tggtctatct
     2221 gcataaattt taaacatttc atttgtagct tcaagtagca ctaacacatc tctttcattt
     2281 tgcggcataa cagctacttt gggtaaatct gcgcccaatt cctgcattct acataaacga
     2341 gatacaatct cttctttttt tggagtttta ttaaaatcat gattagatat aattactttc
     2401 acttcttttt tatgagcaaa attaactact tcatctatga cctcatctcc cataaataac
     2461 tctacatcta ttaaatctac taaacctgta ttagaaattt ctttattcaa agtagtataa
     2521 taatctcttg atattaattt ttctccccct tctactacac ttctaaatgt gaataataaa
     2581 gggatatcat gtatataact tcttaattca tagagaactt ccttaacttc tttaatattt
     2641 tctacattct caaaaaaatc aactcgccac tctataatat ccaaacatgc atcttttaac
     2701 tctttagcct cttttataat atctttttta tttttcccaa taattggtac acaaatttta
     2761 ggtctacctt ctccaatagt aatatttttt acttgaactt ttcttttcat attctcctcc
     2821 ataatcagaa attctatagg actttaaatt ctattttctg cataatttaa ttatacatta
     2881 aaaatactat acattataaa aatactaatt taaaaataat ttttattcta attcttaaaa
     2941 caatcccaca tattttagaa aaggtactct ataatataca aacacttctt ttttacctaa
     3001 atttatttta acattctata attttcacaa tattctttaa attttactca tataaacaat
     3061 attattctgg atgctaatag aattaatcta taaatcatag tataattatg cagaaataac
     3121 tcgaatttca ctacttataa agagattgaa tattaatgaa tctaaatcat ttgcattact
     3181 tcagagtttt gactaaagtt gaacaccata caaaagtagc tactcaattg tttatacccc
     3241 aaccaagctt aagtaatgtt atatagattc ttgaaaaaga atttggtatc tttttatttg
     3301 aaaaacaagg tagaaatgtt tccctcacta aatacggaag tttttttcta acctatgttg
     3361 ataaagcatt aggttaatta gaaaaagttt acatataaaa tatattaaat tgttgcaagt
     3421 tttattgttt tgatatacaa tcaactaaac atataatctt tataaaatac tatacagtta
     3481 aaagtctgtt ttaaaaaaaa atgtataaat tataaatcaa aatatagata ctatcaagga
     3541 gtgattctgt gaagagatgt attgtagtag tggaatagtc aaaaacataa tatatattaa
     3601 aggagagatt taaatatgac tattccaaat tcaaataagg tatatccaag aagtaatgat
     3661 tatcaaactg tatatcttaa aaatgtaata acaagggaaa atatagaagt tggagattac
     3721 acaatatata atgactttta taatgaccca agagattttg agaaaaacaa tgtattatat
     3781 cattacccta taaacaatga taaattaata attggtaagt tttgctcaat agcatgtaat
     3841 gcaaaatttc taatgactag tggaaatcac tcaatgaaat caaaatccac atatactttt
     3901 cccatctttt atgaagaatg gaaactcgat atcaaccata taacggacgc ttgggacaat
     3961 aagggtaaca ttataattgg aaatgatgtg tggataggtt atgattctat aattatgtca
     4021 ggtgtaaata ttggtgatgg tgcaattatt ggaacaagag cagtagttac taatgacgtc
     4081 tcaccgtatt ctattgttgg aggagtacct gcaaaaatta taaaaaagag atttaataat
     4141 gatacaattt caaaattatt agaaattaaa tggtgggatt ggccatataa aaaaattcaa
     4201 tcaaacatta agcatattca atcaggaaat attgataaat tagtttaata ttttataatt
     4261 tatttatatt aaactttact ttaataatcc ttttataaga gctttttcaa aatttgtata
     4321 ttattttaat atatataaat ttaatacaac attaaataaa ataaaaagta aagttactta
     4381 ataaaaagtt actttacttt ttattattta aattcctgtt tttattat
//
LOCUS       DAELXX010000194         4396 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.196, whole genome shotgun sequence.
ACCESSION   DAELXX010000194 DAELXX010000000
VERSION     DAELXX010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.196"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..147)
                     /locus_tag="KPH29_003577"
     CDS             complement(<1..147)
                     /locus_tag="KPH29_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1469138.1"
                     /translation="MDNFLFNVLASLTASVVVYLISKLFKKAKSHSRMNYTPLRRQLK
                     YVKLS"
     gene            complement(290..1717)
                     /locus_tag="KPH29_003578"
     CDS             complement(290..1717)
                     /locus_tag="KPH29_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp26"
                     /protein_id="HBG1469139.1"
                     /translation="MKIKKLLTIGLSLSIFIASCPISANALDKIESIKGADKYETAGI
                     IADKQNYTTAILINADSTMADGLSASGLAGAINAPILLTKKNNIPNATLKRLEKAKKV
                     YIIGGENSINKYTETVLKGKGIEIKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAF
                     KGEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMNDKLVKDTNSTR
                     LGGTDRYDTNKKIINKFYSGVKEFYIASGTDLVYALVGSTIAKNNAIVLVGNDSNKSV
                     LKSTIKLTAIGNLSDSILQQCLNVTKNIGDSNTGREYTVEDAANAVKKLLGVKKFYYE
                     PNYSEDEDLIFLLDKNFDFSGKEYYVFAYIKQGAEGDVRYCVAKNDMSKVYTYDVNGN
                     MKVCNPDDYKNKGSITEEQAKQIALKECAKRHKVSINNLVVDMIDFRDDIYSGNVYAV
                     KINSKNSNGTFGWFFIDIETGKIYN"
     gene            1908..2729
                     /locus_tag="KPH29_003579"
     CDS             1908..2729
                     /locus_tag="KPH29_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG1469140.1"
                     /translation="MIISKKITAILVSFLIMISMVGCAPKESATEVAKKYLDSIEIKD
                     YEVAYNLLSKDSKESVSLEKFEEYQDTLFKLQTLKDYKLGEENKVEKYKYSNKEYTNL
                     VKIKETDTIKYITENKEDTANFDRYFIIEDNEYKLLWHKDFKRALSSNYVKIARSETK
                     KDEDKDYNKAAIDIKKAIEIDDKNPLAYYTQSYIYIELQRYDEALKSIDKSLEYLDKN
                     NEAYKIDASDAYNLRGVIFMSKSEFNKARESYNKALELNPDNEYAKTNLNDIKGL"
     gene            2854..4371
                     /locus_tag="KPH29_003580"
     CDS             2854..4371
                     /locus_tag="KPH29_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG1469141.1"
                     /translation="MSVAIYLRKSRADEEAEKQGEFETLSRHKSTLLKLAKEQNLDVI
                     EIKEELVSGESIIHRPKMLELLKEVEENKYDAVLVMDLDRLGRGDMKDQGIILETFKE
                     SKTKIITPRKTYDLTDEFDEEYSEFEAFMARKELKLISRRMQRGRIKSVEEGNFIGTS
                     APFGYDAVTTGRKERILVPNKDADVVRTIFDLYINEDMGCSKISKYLNNLGIKTATGA
                     NWYNSAITNIIKNKVYCGYIQWQKKDYKKSKNPNKIKTVKLRPKDEWIEAKGKHEPLI
                     SEITWKKAQNILKKNGHVSYGNQIKNPLAGIVICKNCGRPLVYRPYADHDYIICYHPG
                     CNKSSRFEFIEAAILKSLEDTVKKYQLKACDLDLDKNNKDSNIEFQKRVLKGLETELK
                     ELGKQKNKLYDLLERGIYDEDTFIERSNNISSRTEEIKDSIKTVKNRLSTVKKDNSKI
                     IEDIKTVLSLYHDSDSLGKNKLLKSVIDKAIYYKSKEQKLDSFELMVHLKLHEDQ"
BASE COUNT         1710 a          611 c          604 g         1471 t
ORIGIN      
        1 agacagtttt acatatttca actgtctcct aagaggggta tagttcatgc gagagtggct
       61 ttttgctttt ttgaatagtt tactgattaa gtaaactacc acactagctg ttaaactagc
      121 taaaacatta aacaaaaagt tatccatgaa ctcacctccc ttcgaatcgt tgggaggata
      181 atcttttgta catgaactcc actctataga ttgtaagtta aatcttcttg ctaaaaatat
      241 tataacatat tatggtaaat tatataatta ttgtttaaaa gttctaattt taattataaa
      301 ttttcccagt ttctatatct ataaaaaacc aaccaaaagt accattacta ttttttgaat
      361 tgattttaac agcatagaca ttaccactat atatatcatc tctaaaatct atcatatcaa
      421 ctactaagtt attgatactg actttatgtc tcttagcaca ttcttttaat gctatctgtt
      481 ttgcttgttc ctctgttata cttcctttat ttttataatc atctggatta catactttca
      541 tattaccatt aacatcatat gtataaactt tgctcatatc atttttagct acacagtatc
      601 taacatcacc ctctgcaccc tgtttaatgt aagcaaatac atagtactct tttcctgaaa
      661 aatcaaaatt tttatctagt agaaatatta aatcttcatc ttctgaataa ttaggttcgt
      721 aataaaattt cttcacacct aatagttttt tcactgcatt agcagcatct tctactgtat
      781 attctcttcc agtattagaa tcgcctatat tttttgttac atttaaacac tgctgtaata
      841 tactatcact caaatttcct atagcagtta atttaattgt acttttcaat acacttttat
      901 tactatcatt accaactaac acaatagcat tatttttagc aatggtagaa ccaacaagag
      961 catacactaa atcagtacca cttgctatat aaaattcttt tactccacta taaaatttat
     1021 ttattatctt tttgttagta tcatatctat ctgtcccacc taatctagtt gaattagtat
     1081 ccttaactag tttgtcattc attgatgaag taccacctat tacataactt tctacaccag
     1141 ttgtattaaa tggaacactt tttccatcag ttaatactat agctgcttta tctctaactg
     1201 ctacaggtgc agcactcatt gcatcaggct ctcctttaaa agcattagtc aatatgactt
     1261 tatttacttt gttaattgaa tttatttctt ttgcaacatt atagcttgtc ttaattctat
     1321 cactaccttg aagtctttta atctctattc ctttgccttt tagaacagtt tcagtatatt
     1381 tattaataga gttttctcct ccaattatat atactttttt tgccttttct aatcttttaa
     1441 gtgtagcatt tggtatgtta tttttcttag tcaaaagaat tggagcgttt atagctcctg
     1501 caagtccact tgcacttaat ccatcagcca ttgtactgtc tgcattaatt aatatagctg
     1561 tagtatagtt ttgtttgtct gcaattatac cagcagtttc gtatttatct gcacctttaa
     1621 ttgattcaat tttatcaaga gcatttgcag atatagggca actagcaata aatatagata
     1681 aagataaccc tattgttaat aattttttaa ttttcataat atctccttct cttaaatatt
     1741 ttcacctaaa ttatataata cttttcagga aaatgttacc ataaatttta aaaattatta
     1801 ttacttaata taatattaat gaaaaatatc attcttaaat taatgacttt ggtagaatat
     1861 ggtgataaaa taaaattagt gagagacaat ataaggggga ttatattatg ataatcagta
     1921 aaaaaataac agcaatatta gtatcatttt taattatgat atcaatggtt ggatgtgctc
     1981 caaaagaaag tgctacagaa gtagctaaaa agtaccttga tagtatagaa atcaaagact
     2041 acgaagtagc atataattta ttaagtaaag acagtaagga aagtgtcagt ctagaaaaat
     2101 ttgaagaata tcaagatact ttatttaaac ttcaaaccct aaaagattat aaacttggtg
     2161 aagaaaataa agtagagaaa tacaaatatt ctaataaaga gtacacaaat cttgttaaaa
     2221 taaaagaaac tgatactata aagtatatca ctgaaaataa agaagatact gcaaattttg
     2281 atagatattt tataatcgaa gataatgaat ataaattatt atggcataaa gactttaaaa
     2341 gagctcttag ctctaattat gtcaaaatcg ctaggtcaga aacaaaaaaa gatgaagata
     2401 aggattataa taaagctgct attgatatca aaaaagctat agaaattgat gataaaaatc
     2461 cacttgcata ttatacacaa tcatatattt atatagagtt acaacgatat gatgaggctt
     2521 taaagtcaat tgataaatcc ttagaatatc tagataaaaa taatgaagct tataaaatag
     2581 acgctagtga tgcttacaat ttaagaggtg ttatttttat gagtaaatca gaatttaata
     2641 aagctagaga atcttacaat aaggcgcttg aacttaaccc ggataatgaa tatgcaaaaa
     2701 ctaatctaaa tgatattaaa ggattataag gcattaatat tatagaaaat aagtatgttg
     2761 aaagttaaat taacttttga catgcttatt ttctatattt taaactctat tgatttacta
     2821 tataatagtg cataaggagt gtgattactt attatgagtg ttgccatata cttaagaaaa
     2881 tcccgtgctg atgaagaggc agaaaaacaa ggcgaatttg aaactttaag tagacataaa
     2941 tcaaccctac taaaactagc aaaagaacaa aacttagatg taattgagat aaaagaagaa
     3001 ttagtttctg gtgaaagtat aatacataga cccaaaatgc ttgaacttct aaaagaagtt
     3061 gaagaaaata aatatgatgc agtcttagta atggacttag acagacttgg tcgtggagac
     3121 atgaaagacc aaggtatcat attagaaaca ttcaaagaaa gtaaaaccaa aatcatcaca
     3181 cctagaaaaa catacgacct aacagatgaa tttgatgagg agtattcaga atttgaagca
     3241 tttatggcaa gaaaagaatt aaaacttata tcaagaagaa tgcaaagagg tagaataaaa
     3301 tcagttgaag aagggaattt cataggaact tctgctccat ttggatatga tgctgtaaca
     3361 acaggtagaa aagaaagaat acttgtacca aataaggatg ctgatgtagt taggactatc
     3421 tttgacttat atattaatga agatatgggt tgtagtaaaa tatcaaaata cctaaataac
     3481 ttaggcatta aaactgctac aggtgctaat tggtacaatt ctgcaattac aaatataata
     3541 aaaaataagg tatattgtgg ctacattcag tggcaaaaaa aggactataa aaaatctaaa
     3601 aatccaaata aaataaaaac tgttaagcta agacctaaag atgaatggat tgaagcaaaa
     3661 ggaaagcatg aacctttaat aagtgaaata acatggaaaa aagctcaaaa tatacttaaa
     3721 aaaaacggtc atgtcagcta tggtaatcaa ataaaaaatc cacttgcagg aatagtgatt
     3781 tgcaaaaatt gtggtagacc attagtatat agaccatatg cagaccacga ctatataatt
     3841 tgttaccatc ctggttgcaa taaaagttct cgatttgaat ttattgaagc tgccatttta
     3901 aaatctttgg aagacacggt aaaaaaatat caattaaaag catgtgattt agacttagat
     3961 aaaaataata aagatagtaa tatagaattt caaaaacgag tcttaaaggg cttagaaact
     4021 gaattaaaag aattaggtaa acaaaaaaat aagttatatg atttattaga acgtggaata
     4081 tatgatgaag atacatttat tgaaaggtcc aacaatataa gttcaagaac tgaagaaatt
     4141 aaagattcaa ttaagacagt aaaaaataga ttgagtactg ttaaaaaaga caattctaaa
     4201 ataatagaag atattaaaac tgtgttatca ctttatcatg attcagattc ccttggaaaa
     4261 aataaactcc taaaatctgt aattgataaa gcaatttatt ataagtctaa agaacaaaaa
     4321 ctagattctt ttgaattaat ggttcatttg aaattacatg aagaccagta ataccaataa
     4381 catataaaga gtagtg
//
LOCUS       DAELXX010000195         4217 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.197, whole genome shotgun sequence.
ACCESSION   DAELXX010000195 DAELXX010000000
VERSION     DAELXX010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.197"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            287..1159
                     /locus_tag="KPH29_003581"
     CDS             287..1159
                     /locus_tag="KPH29_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1469142.1"
                     /translation="MEMKIAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTT
                     FMRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVL
                     SDIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEP
                     TAGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILDSGKVIYNGSTEA
                     LAKLAEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPT
                     IEDGYMLIMGGDNI"
     gene            1156..2445
                     /locus_tag="KPH29_003582"
     CDS             1156..2445
                     /locus_tag="KPH29_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1469143.1"
                     /translation="MNLFIKECKNILKSMVYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIDEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKSISEINLIWKTDESIKIDYSKVSLERFEE
                     IMQRVDDILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFIMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWLYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLGALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            2375..3019
                     /locus_tag="KPH29_003583"
     CDS             2375..3019
                     /locus_tag="KPH29_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1469144.1"
                     /translation="MFGQSFQNCLVLIKENFKLSTLTNLICSIILLCMIPLIQGVANL
                     DRISTAICLENYVAIIGIVMLVPVFSLEQSKEIDEIVSSKSLSQVIVQLIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYIIGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPIKRKIR"
     gene            complement(3286..3750)
                     /locus_tag="KPH29_003584"
     CDS             complement(3286..3750)
                     /locus_tag="KPH29_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG1469145.1"
                     /translation="MNLNKVKFTEEHAIEVTNWKYEGKYSIYNLPPWEEIKKKNFSLS
                     KEDKRKNFISFINDNKELIGFINLLDEGSYVFFGIGIKPDYCGMGIGKEIISLGLQEC
                     RNKYSTKPIVLNVRTWNMRAVKCYKSQGFKIVETQVQKTHLGDGEFFVMRYQ"
BASE COUNT         1606 a          491 c          619 g         1501 t
ORIGIN      
        1 tcttattata tccagtaatt cttttggata cttatcacat ttttcattat atataaaaat
       61 agactcacaa ataaaataaa tatatattaa attatagctt cataaactta aaaaatttca
      121 gaaacaaaga tactttaatt taattaaaaa cagaaaaatt aaaatcttta gaaaaaattt
      181 agaaatttat aaataatatc ttaagtttta gaatttatta tgaaaataca gtaaaaattt
      241 ataaaaataa aattctataa aattatttct gggggtgtat ttatatatgg aaatgaaaat
      301 agcagtatcc aatcttagta aaagatttgg gaaaaaacaa gctcttgata atataaatct
      361 tgaaatcgac agtggtatgt ttggtctttt aggaagaaat ggagcaggaa aaaccacatt
      421 tatgagaatt ttagccactt tattatcaaa aacagaaggt tcaatacata tgtgtggagt
      481 tcctattgag aatactaagg aaattagaaa tatggttggt tatttgccac aagatttttc
      541 aatgtatcct aacatgacag tatataaagc aatggattat ttagcagttt tatcggatat
      601 accactaaga aaaagaagaa aaattatagc aaagttatta agcaaagtaa accttactaa
      661 ttatcaaaat gtgaaagtaa aatctttatc tggaggtatg aatagaaggc ttgggatagc
      721 acaagcatta attcataatc ccagagtttt aatagtggat gagccgacag caggtcttga
      781 cccagaagaa cgtataagat ttagaaattt actatgcgaa attgcaaatg atagaatcgt
      841 tattttatct acacatattg ttggagatat agaagctaca tgtgaaaacg tagctatatt
      901 ggatagtgga aaagtcatat acaatggctc aacagaagca ttagcaaaac tagctgaagg
      961 gaaagtatat agtattgaag tagataaaaa agacattgaa aatataaaaa gtagatttat
     1021 agttatagga atgttgactc atggtggaaa ggctatactt aggattatat cagatgataa
     1081 accttttgag acagcagtaa attgtaatcc aactattgaa gatggctata tgcttataat
     1141 gggaggagat aatatatgaa tctatttata aaagaatgta aaaatatatt aaagagtatg
     1201 gtatattata tttttattgt agttttagtt tttttctata tttctcaact tggaagttca
     1261 gtttcaaatg atgtaagtaa aaatattcct aatgatggaa atccattagt tgctccacaa
     1321 aaagtagaga atattgacga atattactct gtacacaatc aatatccata tggtcaaaaa
     1381 gaatctatga aaacagaaaa ggtcattcca aatgcaacta taaacctaat tagagaatat
     1441 caaaaaaaca catatacaac gtatccaaca ggggtagtta agtatataaa attagaccaa
     1501 aataaaacga agaccataga aaaatatata gaagaaataa ctggtaaaag tattagcgaa
     1561 ataaatttaa tttggaaaac tgatgaaagt ataaaaatag attattctaa agtatctctt
     1621 gaaagatttg aagaaataat gcaaagagta gacgacattt taggaggagg gtctgcatat
     1681 gcttttgaca agttacaaag atttggagta gttccagtta cctatgatga agctatgaag
     1741 aattacaact atacaataca aaaagataaa gttagtaatg ggtatgcaag aattttttgc
     1801 gattatatag gtatagtttt aagtatattt ccagtatttg tggcagtttt tatgtcactt
     1861 aaagataagc gttcaagaat gagagagatt atttattcaa aacaagtatc ttcatgcaaa
     1921 atagttttat caagatattt tgcacttgtc tttataatga tattgcctgt attaatttta
     1981 ggtatgaaag atttacttcc tttgattttc tttgctaaag ataaaggtat atctattgat
     2041 aacttagcat ttataaaata tacattatgg tggatactac caactcttat gatagttaca
     2101 gctgtaggaa tgagtattac tatattaaca gatacaccag tagcaattat tgtgcaatta
     2161 atttggtggc tttacagtat aaattctaca ggactcaaag gtgattattc aacaatgggg
     2221 ctaatgataa gacataatag tgtattaaat ggggatatta ttatacaaaa cttcaatcaa
     2281 attatgatta atagaattgt gataagcttg ggagcattgg tgcttgtttt gttagctata
     2341 ttcttatatg agcaaaaaag aagggggaat cttgatgttt gggcaaagtt ttcaaaactg
     2401 tttagttcta ataaaagaaa acttcaaatt aagcacttta actaatttaa tatgttcaat
     2461 tatattgcta tgtatgattc ctctaataca aggagtagct aatttagaca gaatttcaac
     2521 agctatatgt ttagaaaatt atgttgcaat aatagggata gtaatgttgg tacctgtatt
     2581 ttcgctagag caatcaaaag aaattgatga gatagtatct tcaaaatcat tatctcaagt
     2641 tattgtacaa ttaattagga tactgatgtc aattataggt atagtggtat taatcatagg
     2701 ttttgtaaat gttttaatat atcttggatg taattttcca gtagttgagt atattattgg
     2761 aagtttcgca tcagctatgt ttataggaag tttaggaatg tttttctcaa atttatttaa
     2821 taatacaatc ataggttaca tgatatctat aggatattta attttaaata tgatgactaa
     2881 aggcaagtat gttggaaaat tttttataat gtctatgagt caaggtagtt ttgatgagaa
     2941 atattggctg atacttggaa gtgttgtgtt gataattgca tctctcttaa taaaaccaat
     3001 aaaaaggaag attaggtaat aaaatgagag cttagataaa ataaaggtaa tacaaataaa
     3061 aagtacattt atgaaatata aatttatttt tatatacaca aacctactta caatctcttt
     3121 atgtatgtag atagatttat atatctgtag tataaaatat atatgtatac cgaattttat
     3181 caaaggctct caaatttata tttcaaagac acttattgct atgacataca cacatgtatt
     3241 ttttcatttt aattgtcata ttagcaatat gactagtgtt ataaactatt gatacctcat
     3301 aacaaaaaat tctccatccc ctaaatgcgt tttctgtacc tgagtctcaa ctattttaaa
     3361 tccttgagac ttataacatt ttacagctct catgttccag gttcttacat ttaatacaat
     3421 tggtttagta ctatatttat ttctacattc ttgtaatcct aggctaatta tttctttgcc
     3481 aattcccatt ccacaataat ctggttttat accaatacca aagaatacat aacttccttc
     3541 atccaataag tttataaaac ctatcaattc tttattgtca tttataaaac ttataaaatt
     3601 ttttctttta tcttcttttg atagtgaaaa attcttcttt ttaatttcct cccatggagg
     3661 taggttgtat attgaatatt taccttcata tttccaatta gtaacttcaa tagcatgttc
     3721 ttcagtaaat ttaactttat ttaaattcat atgtcctcct attaaaatat ttaaggtaat
     3781 taatggtatt ttaaatttta caaaataagt aaaactattt atatctatcc ataaaatctt
     3841 taaaaatttt actaacaatt cttgtaccct gtacttttta ttggattcac agaagactat
     3901 aaactaattt tgaattacct tctaattcaa aaacatcacg atatatgtaa tttcttagct
     3961 tgattaaatt ctgttaaatc tgtttcttat agttgtataa tcaacatcaa tatctttatg
     4021 tgtattagtt aatttattgg ttttaggaac ttattattaa tatattgtat ttgctccaat
     4081 actccaagta gtaggtaaat tataggcaaa ttatcaaatg tatatctatc catgcttaaa
     4141 ctttagagat acaataacta ctaaattagt attctagtag ttataaaaaa gaatctttaa
     4201 aaatattaat ttaactt
//
LOCUS       DAELXX010000196         4177 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.198, whole genome shotgun sequence.
ACCESSION   DAELXX010000196 DAELXX010000000
VERSION     DAELXX010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.198"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            421..1695
                     /locus_tag="KPH29_003585"
     CDS             421..1695
                     /locus_tag="KPH29_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBG1469146.1"
                     /translation="MQFMDVSKYIEGANLKVKTKNGETVKRIYFNNSATPLVLKNVVD
                     NLNCEIPWLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSAIYVKNATEGINF
                     LAKFFKDENPDKFIITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYANVPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYAKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGISPKEAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
     gene            complement(1902..2219)
                     /gene="trxA"
                     /locus_tag="KPH29_003586"
     CDS             complement(1902..2219)
                     /gene="trxA"
                     /locus_tag="KPH29_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBG1469147.1"
                     /translation="MVKIINNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     NEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKNKIE
                     SYM"
     gene            2486..3238
                     /locus_tag="KPH29_003587"
     CDS             2486..3238
                     /locus_tag="KPH29_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HBG1469148.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLQILNKN
                     KSVEYVLNIKGDKNILKKALEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLKGLDNIHTHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            3225..4106
                     /locus_tag="KPH29_003588"
     CDS             3225..4106
                     /locus_tag="KPH29_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBG1469149.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDASNISDEIKQNVAKEMN
                     LSETAFVSESNVADFKVQFFTPGLEVDLCGHATIGTFHALLEEGRLNTNKSIFYQETK
                     AGVLSVELKEINNENIFMMEQNTPEFENLDNYRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNDITS
                     VKIGGSAYTVLSGEIMY"
BASE COUNT         1634 a          547 c          568 g         1428 t
ORIGIN      
        1 gtacatcttt tataacatat aaaatctttt tttctaatta tatacaatat attttccatt
       61 atgttctaat caatttcttt actattttta caactttatt agtcatattc ataattattt
      121 ctaattagtg tttaacattt tatgtaaaat ttataaaaaa atgtatataa attaaaataa
      181 gtgactatac taaaaatatg atacggataa acaataagtt caacctatct cccccaaaat
      241 caagtgagct acagtttccc ccaactgtag ctcattttcg tgtaatttaa taaacatctc
      301 aacaagaatt aattaacaaa accccttata aaaacataac atatattaaa caagctcttg
      361 attaaaactt gagctttttt aataaaaaac agaatagttt attagataag gggttttttt
      421 atgcaattca tggatgtatc taaatacatt gaaggtgcaa accttaaagt taaaactaaa
      481 aatggtgaaa ctgttaaaag aatatatttt aacaactctg ccactccatt agttttaaaa
      541 aacgtagtgg ataacttaaa ctgtgagatt ccttggctta cttatattaa tgctccagga
      601 ataatctcag aaaaaaatac tttaaaatat gaaaatgtac gctctactat cctcaaactt
      661 attgatggag acgaagaaaa ggattctgct atttatgtca aaaatgctac agaaggaata
      721 aacttcttag caaaattttt taaagatgaa aatccagata aatttattat taccacagca
      781 atggagcata tggctaatta cctacctttt aaagttaatt ttcctacaaa agttgttgga
      841 attacagaaa aaggagaact tgatttatgt caattagaga atatacttaa aaattatgct
      901 ggtaatgtat ctctcgttac agttacagga gctagtaatg tcacaggtat aaccactcct
      961 atatatgaaa tagcaagaat agctcataag tatggtgcaa agatattggt ggatattgtg
     1021 caagtaattc agcataaacc tttttcaatg atgccacatg aatgtgatga gcatatagat
     1081 tttgttgttt ttagtgctca taaatgttat tctccactaa atggaggagc tctggtagga
     1141 cctaaaaaat tctttgaaaa atttaatcct gtactatatg gctcaggttc taccaaattt
     1201 gtaaatgata acaaaatact atatgcaaac gtaccccaac gttttgaagc aggataccca
     1261 gagtattttg gaactcttgc aatgggaaaa gcactgaaca ctttaaataa aattggtcta
     1321 aacagaattt caaaatacga aagagaatta tttttatatg ctaaagaaga aatgagtaaa
     1381 atacctaata taataatata tggagataaa tcaaataatg taattatccc aaatatatct
     1441 tttaatataa gtaatatgta ttacaaagat gtagcaaaat atttagttga taactttgga
     1501 atagaaactg gagctggagt agtaggtgca gatatttatg tacaaaaatt attaggtata
     1561 agtccaaaag aagcatacgt cagatttatg agggaaaatc ctgtaggtct tgttaggata
     1621 agcttaggaa tgtacaatac ttttgatgag gtggatagct ttatacatgc tattaaaact
     1681 ttggctttta aataagcttt ttctattctt ttttatcaat aaatagagag tagttttgtt
     1741 ttcacaaaaa aacaaattat atcaacagtc aatttcatta gcaattagac actcatttgt
     1801 aactatacaa actattttac actatacaaa taaaaaactc attacaatga gtttttttgt
     1861 ttatatatga tatttatttt aattacttca atttacttta attacatata tgattcaatt
     1921 ttgtttttta aattctcctt aggcataaaa cctattaatc tatctacagg ttttccatcc
     1981 ttaaatatta gcatagttgg tactgtagat acttcaaatt tctgagctaa ttctatactt
     2041 tggtctatat ccaccttcaa gaagtttgct ttttcaacca tctcatttcc taaagcttca
     2101 tatattggtg atagcatctt acaaggtcca caccaagtcg caaagaaatc tacaactact
     2161 aaaccatctt tattttctac ttcatttata aattcattat tatttattat ttttaccata
     2221 atatacatct cctttattat cttcttgata ctaatatact acgccctata taacagttca
     2281 attaggcact tttttgtaac cgcctatttt taatagattt aatattatga taaatagatt
     2341 tataagatta aatatttata agactaaata tttctgatat attttatttt tttcttaata
     2401 tagttgacat tttctccctt aaattataaa atcaaattaa taactacata ggtagttact
     2461 aatcttttat caaaggagtt tctatatgga taatataatt aatgaattac aaaatatagg
     2521 atttactaaa tacgaatctc aaatatacat atcattatta aaggaaagcc ctttaacagg
     2581 atatgaaata agtaaattat caggtgtacc acaatcaaaa gtctatgaaa atataacaaa
     2641 gctattaaac aataatataa ttatcaatgt tggaactgac cctattaagt acatacccat
     2701 aaaccctaat gaattattaa aaaataagaa aaaagaattt gataattcta tggataatct
     2761 aaagaatagc ttacagatat taaataaaaa taaaagtgta gaatacgttt taaacataaa
     2821 aggagataaa aatatattaa aaaaagcatt agaaatgata aaaaatgcaa aacaagaaat
     2881 tataatttct tgcccttatg aagaaattct tgaattaaaa aaagaactcc taattgctta
     2941 caaaaaaaat attaatattg aaatactact tttagaaaac aaaagtctga aaggtcttga
     3001 taatattcat actcatggag gtaataaaac tacacttgaa aataaaatgc tttacaaagg
     3061 aattattttg attgtagata atgatgaaat attgacagga aacttatcag aaggcagtga
     3121 ggccatttca atttggacaa aaaactctaa cattttatat attggagttc aatacataaa
     3181 acatgaaatt tatatttcag aacaaataaa aggaggttat aaaaatgaac aaaattaaga
     3241 ttaaacaagt tgatgctttt acaactatac cttttggtgg aaatcctgct ggtgtcgtta
     3301 ctgatgcctc taacatttct gatgaaatta agcagaatgt ggctaaagaa atgaatttat
     3361 cagaaactgc atttgtttca gagtcaaatg ttgcagattt taaggtacaa tttttcacac
     3421 caggtttaga agttgattta tgtggtcatg caacaatagg tactttccat gcacttttgg
     3481 aagaaggaag gcttaatact aataagagta tattttatca agaaacaaaa gctggtgtat
     3541 tatctgtaga gcttaaggaa ataaataatg aaaatatatt tatgatggaa cagaatacac
     3601 ctgaatttga aaatttagat aattacagaa aagaacttgc acaaatgata ggtgtgcatg
     3661 aagatagact tcttgaatat cctattataa aggtaaatac tggactttgg tggcttgtat
     3721 ttggtctaaa atctttagat gatttaaaaa atatctctcc agatttaaat aaaatcaaaa
     3781 aatttagtgg tgataataat ataattggta taactccatt ttgtatagaa actatagata
     3841 aaaagtgtga ctaccattta agatctatag caccatatgt tggagttaca gaagaccctg
     3901 tatgtggaac tgggaacgct tgcgtatcat cttatatagt acatcacaat attttagata
     3961 aaactagttt tattggtgag caaggtaact ttataaacag acctggaaag gtatatgtaa
     4021 atataactag agaaaacaat gacataactt ctgtaaaaat tggaggaagt gcttatactg
     4081 ttttaagtgg tgaaattatg tattaatccc atacctaata aaaacaaata aaaatactag
     4141 ctaactaaag ctagtatttt tatttgttat ttttatt
//
LOCUS       DAELXX010000197         4082 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.199, whole genome shotgun sequence.
ACCESSION   DAELXX010000197 DAELXX010000000
VERSION     DAELXX010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.199"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            164..2263
                     /gene="nrdE"
                     /locus_tag="KPH29_003589"
     CDS             164..2263
                     /gene="nrdE"
                     /locus_tag="KPH29_003589"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBG1469150.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLRKFN
                     NLEERLKYLIDEGYYLKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGDKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDEWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            2276..3247
                     /gene="nrdF"
                     /locus_tag="KPH29_003590"
     CDS             2276..3247
                     /gene="nrdF"
                     /locus_tag="KPH29_003590"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBG1469151.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKKMLEEKMYSILYSLMENEIEYTNVIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            3323..3949
                     /locus_tag="KPH29_003591"
     CDS             3323..3949
                     /locus_tag="KPH29_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG1469152.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIIEIPEQKFIMLKGSGDPNSKEFI
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLIRKSYTHREIYISDARKVSPESLKTVLRFEVEREV"
BASE COUNT         1630 a          445 c          763 g         1244 t
ORIGIN      
        1 ataaagcact gactatatgt agtgcttttg tttttgaaaa atacttattg catttctata
       61 attatagatt aattttggga tttcaactga taagtaccaa aagaaataat aagcaaatta
      121 agtgaaatta tggcacaaga ttatgttaaa aggacggtaa ataatgaatt ggatagagct
      181 taacaatcaa gtgataatta aaaatgaaca aggtaagtac caattggaaa aggataagga
      241 agccttaagt agttacatag aagaatttat acaaccaaag ttgagaaaat ttaataacct
      301 agaagaaaga ttgaagtatt taatagatga aggttattat ttaaaagaag taataaatca
      361 gtatagcata gatgttatta aaaatatata tgatatgatt gataaacata agtttgaatt
      421 tcaatcatat atgagtgcaa ataagtttta tcaaaattat gcattaaaga gcaatgatgg
      481 aaaggaaata ttagaaacat ataatgataa agtgttaatt gtagctttga cactaggaaa
      541 tggcgataaa gatttagctt taaacttggc agataaaata gtaaaacaag aatttcaacc
      601 agctacacct acttttttaa atgctggaag aaagagggct ggagaaatgg tatcatgttt
      661 tttactatct gtagaagata gtacagaagg tatatcatat gctatatctt catcaaatca
      721 cttatcaaag attggtggag gagttgcact aaatttatct aaacttagag catctggcga
      781 atctatcaaa gatatagaag gtgcagctgg aggtgttgtt ggagtagcta aaatgttaga
      841 acaatcattt agttacttta atcaaatggg agctagacaa ggttctggtg cggtgtacct
      901 tactgtattc catcctgatt ttgaattgtt gatggatact aaaaagataa atgcagatga
      961 aaaaataaga cttgctacat tatcattagg agctataata ccagataaat ttatggagtt
     1021 ggcagaaaaa aatgaagtag catatgcatt ttatcctcat actgtgtata agaaatatgg
     1081 agtttctctt gatgagatag aaatggatga gtggtatgat aaattggtta atgatagtga
     1141 tataagaaaa aaagaaataa atcctaggca gatgttgaca aaaatagcac aaatgcaaca
     1201 agaaagtgga tatccatatg tagtgtacat agatacagct aatagagaac atacattaaa
     1261 agatgttgga attattaaaa tgtctaactt atgttgtgag atatttcaat atcaaacacc
     1321 ttcagaaata gaggggtatg gaggaaaaaa tgaatgggga caagatataa gttgtaacct
     1381 aggttcttta aacatagcca atgtaatgga taataaaact atagaaagta cagttgaaac
     1441 agcaataaga gcactgtcat ttgtagcaga tagcacagat ataaaaccag tgccaacggt
     1501 aagcaatagt aacagcaagt ctcattctat aggattagga gctatgaatt tacatggata
     1561 cttagtaaga gaaaatattt tatatacttc agatgatgct atagattttt ctaatgtatt
     1621 ttttgcaatg gttagatact attcaattaa agcttctatg aaaatagcca tagaaagaaa
     1681 tcaaactttt gaaggatttg ataaatcaga atatgttaag ggaagaaata gcaaggtttt
     1741 atcaaaatac tatgaacaat cttatctacc taagtcagaa aaggtaagag cattatttga
     1801 agggatttat ataccaacaa aagaagattg gacaaaatta ttggatgaag taaaagaaaa
     1861 agggatatat aatgcatatt taatggcagt agcaccaact caaagcatta gttatgttca
     1921 aaatgctaca tcaagtataa tgccaattac tgagccagta gaagtaagaa catatggaga
     1981 ctctactaca atatatccaa tgccattttt aacaaatgat aatatgttgt attatcaatc
     2041 agcatacagg atggatatga gaaaggtcat agatttagtg gcaacagttc aaaaccatgt
     2101 tgaccaaggt atatcaacaa cactgtttgt tacagatgaa aagacaacta gagatatagc
     2161 tagacattac atatatgcct ataaaaaagg tttgaaaagt ttatattaca caagaacaaa
     2221 gatgacaaga gatactcatg agtgtctagt gtgttcagta tagggagaga aatatatgac
     2281 atttaattta cataaaatac ataatgcagt taactggaat agagaagaag atggatttac
     2341 acaagctttt tgggaacaaa atgtaaaaca attttggctt cctgaagaaa tatctgtttc
     2401 taaagacata aaggtatgga atgaactaag taataaagaa aaggaattat ataaaaaagt
     2461 attgggtggt ttaacattac tggatacaaa acaggggaat aatggaatac catctatgat
     2521 gagtttaaca gaaaacctac aaagaaaagc tgtcttgtct tttatgggga ctatggaaga
     2581 aatacatgca aaaagttatt cttctatatt tatgactcta ctttctaatt tagaaataga
     2641 tgaattattt gaatggatag aaacagaacc aacattacaa agaaaggctg atttagtttt
     2701 agctcaatac gaaaatacta caaatcaaga gggattatac ctatcaatgg ttacaagtgt
     2761 atttttagag agtttcttgt tttattcagg attcttttat ccattgtatc tatctggtca
     2821 aggaaagatg gtagctagtg gagaaataat atcgcttatt cttagagatg agtctttaca
     2881 tggtaagtat ataggattgc tagcacaaga aatatacgat tcatttgata aaatggataa
     2941 gaagatgtta gaagagaaga tgtattctat actttatagc cttatggaga atgagataga
     3001 gtatacaaat gttatataca gagaaagtgg gcttgaaaaa gaggttgtga actttttaaa
     3061 atacaatgcg aatagagctt tagaaaatct agggtttgag aagctatata ctgtagatgc
     3121 tataaatcca attgtattaa atggtcttag cactgaaacc aaaacacatg atttcttttc
     3181 aactaagggt aatggatacc aaaaaggagt gtatgaggaa ttagaggatg aagattttat
     3241 aatataattt ataattatga cacactgttg tcacttttag cgtgataata tcaatgtata
     3301 aaatatatat ggaggtattt ttatgaaata tgaatggaga aaaagagata aagagttata
     3361 tttacctaaa aagaatcctg taataattga aataccagaa cagaaattta tcatgttaaa
     3421 agggagtgga gacccaaatt ctaaagaatt tattgaagca ataggagtac tttattcttt
     3481 ggcatatgca ataaaaacca tgcctaaaaa aggaattgta ccagaagggt attttgacta
     3541 tacggttttt cctttagaag gtatatggga taagggagaa aaaagtaaag gcttagatgt
     3601 acttattaaa gaagatttaa tttacactat aatgatacgt cagccagatt ttgttacaca
     3661 agacctgttg gataaagcta ctgatatagt aaagaagaag aaacctcata atcttcttga
     3721 aaaagttagg tttgggtcta ttaatgaagg gctatgtgtt caaatgcttc atataggttc
     3781 atatgataat gaaccagaaa gttttgacat tatgaacgaa ttttgcaaaa aaaataaact
     3841 tataagaaaa tcttacacac atcgtgaaat ttatataagt gatgctagaa aagtatcgcc
     3901 tgaatcattg aagacggttt tacgttttga agtagaaagg gaagtataaa tattttgaat
     3961 aatttataag agttataaaa agtgaagtct gttgagactt cattttttga tgcaatttta
     4021 aaattaaatt tgatgtttca taatatttta aaaactttct acttttatta gtgtgaataa
     4081 ct
//
LOCUS       DAELXX010000198         3910 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.200, whole genome shotgun sequence.
ACCESSION   DAELXX010000198 DAELXX010000000
VERSION     DAELXX010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.200"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            166..1656
                     /locus_tag="KPH29_003592"
     CDS             166..1656
                     /locus_tag="KPH29_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG1469153.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
     gene            1914..3410
                     /locus_tag="KPH29_003593"
     CDS             1914..3410
                     /locus_tag="KPH29_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG1469154.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACISACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFEFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            3544..3894
                     /locus_tag="KPH29_003594"
     CDS             3544..3894
                     /locus_tag="KPH29_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG1469155.1"
                     /translation="MKKIVLKDILFLVTTLSIIVILLFVTNITTNSKIKSTSESYLKS
                     DKNSVRQNGIQRSTKNTAFKTTILSTGKSDCILIEIGDKFIMIDTGEDKNGEQIVDKL
                     KEKEINALDYLIKK"
BASE COUNT         1548 a          400 c          765 g         1197 t
ORIGIN      
        1 tgaaatttca gaaatatcaa taatattata taataaaata aaaaaacact atatatattc
       61 gataaaataa aatttaaaca tataatattt tatgtatata ttaataaaag gacatattgt
      121 gttataatgg tatcgtataa aatcatttac gggggtggta ttaaaatgtt tcaatttgaa
      181 acacaaatac acaaattaag acatgatgtt ttaactgcaa tagctagact tggaaaagaa
      241 gataacctta ccagagaagc aatggataat attcagtatg aaataataaa aggtgagaaa
      301 cctacataca gatgttgtgt ttataaagaa agagctataa tatctgagag agtaaatgta
      361 agtatggggt tacaaccagg tcaagataag atagatttag atacaatgga tatagaagaa
      421 gatgaacaaa ttatatatat tttggaagct gcatgtgaca gttgtccaat aaatgaattt
      481 acagtaacag atatttgtag aggatgtcta gcacatagat gtaaagaagc ttgtaaattt
      541 ggagctataa cacatgtcgg aggaatggcc tatataaatc atgaattatg taaggcttgt
      601 ggaatgtgta aaaaagcttg tcaatatgat gccatctcag aggttgttag accatgtaag
      661 agtgtttgtc caactaatgc cttaggattt gatagagaaa atatgaaagc tatgatacat
      721 gaggataaat gtttaaactg tggagcatgt atgtcagcat gtccatttgg agcaatatct
      781 gataagagtt tgatagctcc tgtagctaga aagttggttc aaaaagagaa aatgtatgcg
      841 gtagtagctc cagctataac aggtcaagtt gaagcaaatg ttacatatgg tcaaataaaa
      901 aatgctataa aatcactagg atttgttgat gtattagaag cagcttgtgg agcagatgca
      961 gttacagttc atgaaagctt agaatttgct gaaagaatgg aaaagggaga ggcttttatg
     1021 acaaattctt gttgtccagg atttgttggt tatatagaaa aaacataccc aaatcatgta
     1081 ggaaaaatat cttctacagt gtctcctatg atagctagtg gtagatttat aaaaagtcat
     1141 gaaccagatg ctaaagttgt attcatagga ccatgtacag ccaaaaagag tgaggcagct
     1201 aagaaagaat taaaaggagc tgttgattat gtacttactt ttgaagaaat aatggcatta
     1261 tttgaggcat ttgatataga cttggctaag tgttcaaatg aagatataga tgatgcatca
     1321 atttttggaa gaggttttgg agctgctggt ggtcttacaa aagctataga aaattatctt
     1381 gctgaaaaag gtattggtgt taattttgaa cctgtaaaga tttctggttc aagagaaatc
     1441 aagaaaacta tgactatggc taatttagga aagctagaag gtaattttat agaaggaatg
     1501 atgtgtgaag gtggttgtat aaatggttct ggtaaattct tatcaggtgc aaaggttaaa
     1561 gccacttttg atagaaaaaa ttctcaatca actaagaaaa gtgtcttagc aaatacaaag
     1621 ataaaagatt ttagtaatat aaatttagaa aaataaagat aatacaaagc aacaaaaata
     1681 catattgtta atagagttgt tctaagttga ggtttaactt ttggacaact ctatttttat
     1741 ttatatattt aattaatgaa caactaaaat aaaaattaca tatataatat tttgtatata
     1801 acttaacaaa gtatatgcct atgttataat aatttataga aataaataaa aaatacaata
     1861 tacataatag tagaaatata gtaaaaaggt gtgatttata gggaggatta cttatgtttg
     1921 aatttgaaac gcaactaaaa aggttgaaac atgaagtttt aacagcagta gcaagactat
     1981 ctattgaaga aaatttaaca aaagaaaata tagaaaaaat tccatatgaa ataattaaag
     2041 gagaaaaggc gagatataga tgttgtgttt ataaagagag agaagtagtt cttgaaaggg
     2101 cgcaacttgc attaagttta actccaaata gtaaatatgg agatataaat ccagattcag
     2161 ttagtttgga cacagatgag caaattatat atatagttga agcagcatgt gatagatgtc
     2221 caataaatga atttacagtt acagagcttt gtagagggtg tttggctcat agatgtaagg
     2281 aagcatgtaa atttggggca atttcttata taaatgggag agcatatata aatcatgatt
     2341 tatgtaaggc ttgtggaatg tgcaaaagtt catgtcaata tgatgctata tcagaagttg
     2401 taagaccttg taaaagcgtt tgtccaacag gagctttgga ttttaataga aacactatga
     2461 aagcaatgat acatgaagat aattgtataa attgtggagc atgtatatca gcatgtcctt
     2521 ttggagcaat atctgataag agtttgatag cacctgttgc aaaaaaactt gcaaagaaag
     2581 aaaatatgta tgcaatagta gctccagcta taacaggtca aatagatagt aatattacat
     2641 atggtcagtt aaaaaatgct ataaaatcac ttggatttgt agatatgata gaagcttctt
     2701 gtggagcaga tgcagttaca gttcatgaaa gcaatgagct tattgaaaga ataaaatcag
     2761 gagataatta catgacaaat tcatgttgtc cgggattttt taagtatata gagaagatgt
     2821 tcccagatga aaaagataga atatcatcta cagtgtctcc tatggtagca acaggaagat
     2881 tagtaaaaaa aatagattct gatgctaaag ttgtatttat agggccatgt acagctaaaa
     2941 agagtgaggc aacagagaaa agtttaaaag attcagtaga ctatgtactt acttttgaag
     3001 aactaatggc attatttgaa gcatttaata tagatttatc aaaatgttca aatgaagata
     3061 taaatgatgg ttcgattttt ggaaggggat ttggagcgtc tggtggactt gccaaatcag
     3121 tacagaacta tattgctgaa aagggactta gttttgagtt tgaacctata aaagtatctg
     3181 gtccaagaca aattaaaaag acaatgacaa tggctaaatt gggaagatta gaaggtaatt
     3241 ttatagaagg aatgatgtgt gaagaaggtt gtattaatgg agcaggtaag tttttatcag
     3301 gtgtaaaagc taaaaatact tttgatagaa aaaatggaca atctactaaa aagagtgttt
     3361 tatctaatga taagattaaa gaatttgaag atgtaaattt agaaagataa ttgttttggt
     3421 acagtcattt ttttatcatg ccacaaatat gcctaaaatt ttcccttttt atatgtgtat
     3481 tttacagagt taaggttgct atactataaa tatgtaataa ataaaattta taggagcttt
     3541 tggatgaaga aaatagtatt aaaagatatc ctgtttttag ttactacatt atctataata
     3601 gtaatattat tgtttgtaac aaatataact actaatagta agataaaatc aacaagtgaa
     3661 tcttatttaa aaagtgacaa aaatagtgtg aggcaaaatg gtatccagag gagcacaaaa
     3721 aatactgcat ttaagactac tatattaagt actggaaagt ctgactgtat attaatcgag
     3781 ataggtgata aattcataat gattgataca ggagaagata aaaatggaga acaaatagtt
     3841 gataaattaa aagaaaaaga aataaatgca cttgactatt tgattaaaaa atagctacgc
     3901 acactcgtgc
//
LOCUS       DAELXX010000199         3830 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.201, whole genome shotgun sequence.
ACCESSION   DAELXX010000199 DAELXX010000000
VERSION     DAELXX010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.201"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     misc_binding    331..569
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            633..2552
                     /gene="thrS"
                     /locus_tag="KPH29_003595"
     CDS             633..2552
                     /gene="thrS"
                     /locus_tag="KPH29_003595"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBG1469156.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     gene            3320..3748
                     /locus_tag="KPH29_003596"
                     /pseudo
     CDS             3320..3748
                     /locus_tag="KPH29_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1491 a          406 c          703 g         1230 t
ORIGIN      
        1 gtatttactg tactacttat taactatatt aagtattgat tgtatttaag tatacaaaac
       61 ctaaaaagag tacaaaaatt agaagttagt tttaataaaa attagtttta gtatttatct
      121 agtcatatct atcatgcatg tggatacgtt ttaaaaattt taaatttatg aaaatattat
      181 aatatcacta taaatcaaga gattaattta atataaaaat ataagaatat tggtttacag
      241 ctgattgaga tgaaaaataa tgggtaatta atatattaca ttaatgttga ttgatgtata
      301 actatctgtt ataatgaaaa tatattaaaa atcatagtgt tgaagaggaa gagtagagta
      361 gaaaccattc tcagagaatt tgggtctatg gctgagagcc catgtaatag gtctatttga
      421 aaactacctt ggagctgagc ttattagcag tatttgtatt tgtactatac gtgaaagtgc
      481 tcgggtgacg accttatagt catcaagagg gttgtatata cttgtatacg ccaacttggg
      541 tggaaccgcg aattaatatc gtcccaaaca tatttggggc gattttttat tacaaaaaat
      601 ctaaaaatta tttcaataag gaggaaaaaa caatgattaa agttgcttta aaagatggct
      661 caataaaaga atttgaaaat gctatttctg ttatggatgt agctaaatcc attagtgaag
      721 gattagctag aaatgtggta gcagcgtctg tgaacggaga ggttgtaggt ctggaccaca
      781 ttatagatac tgattgtgac ttgaatttat ttaagtttga agataaagaa ggtaaggaag
      841 tttttagaca tacatcagca catatattag ctcaagctat aaaaagactt tacccagaag
      901 caaagcttgc tataggacca agtatagaaa atggattcta ctatgatata gatttagacc
      961 atagattagt tccagaggac ctagagaaaa tagaagctga aatgaaaaaa atagctaaag
     1021 aagatttaaa aatagaaaga tttgaacttc ctagaaatga agctttagag cttatgaaag
     1081 aacaaggaga agactataag gtagaactta tatctgattt acctgaaagt gaaataatat
     1141 ctttctacaa gcaaggtgat tttactgatt tatgtagagg accacattta ccatctacta
     1201 aaaaggtaaa agctgttaaa ttacaaagtg tagcaggtgc atattggcgt ggtgacgaaa
     1261 ataataagat gcttcaaaga atatatggaa cttcttttga gaaaaacaaa gacttagaag
     1321 agtacttaca tttactagaa gaagctaaaa agagagacca cagaaaatta ggtaaagaat
     1381 taggattatt tatgatacct gaagaaggtc caggattccc tatgttttta ccaaaaggta
     1441 tggaacttaa aaatgaatta ttaaaattct ggagagaaat acacagaaaa gctggatata
     1501 ttgagataga atctccaatc atattaaata gaaaattatg ggagacttca ggacactggt
     1561 atcattacaa agaaaatatg tatacagtta aaatagatga tgaggattat gctataaaac
     1621 ctatgaactg tcctggtgga ttaatttact ataactctca actacattca tatagagatt
     1681 tcccaatgag agttgcagag cttggtagag ttcatagaca tgagttatct ggtgcattac
     1741 aaggattaat gagagttaga gcatttactc aagacgattc acatatattc atgttaccag
     1801 agcaaattaa ggacgaaata aaaggagtag ctaacttaat agatggtatc tacaaaactt
     1861 ttggatttga atataattta gaattatcta caagaccaga gaactctatg ggaagcgacg
     1921 aagagtggga agcagcagaa aatggattaa gagaagcttt agaagagtta ggtctaccat
     1981 atactattaa tgagggtgat ggagcattct atggtcctaa gatagacttc catctaaaag
     2041 attgtttagg aagaacttgg caatgtggta ctatccaatt agatatgcaa cttccaagac
     2101 aatttgataa tacttatatt ggtcaagatg gtgaaaagca tagaccagtt atgattcata
     2161 gggttgcttt tggtagtata gaaagattta taggtatatt aatagagcat tatgctggta
     2221 aattcccagt ttggttatct ccaactcaag ttaagatact tcctatatct gataaattta
     2281 tggattatgc aaatgaagtt aagaaagagt tatttgataa gggtataaga gttgaattag
     2341 atgatagagc tgaaaaaatt ggatttaaga taagagaagc tcagcttgaa aaagtgccat
     2401 acatgttaat agtaggtgaa aaagaagtag ctgataataa tgtttctgtt agatcaagag
     2461 ataaaggtga aataggtagt attaaattag atgaatttat agctagtatt tcaaaagaaa
     2521 ttgaatcaag agaaagtatt attcaagact aatatataat atattagtag ctaagatatt
     2581 gttatataag ataagtttaa tcaatatgtt gatatgttga tacatagtga ttaaatttat
     2641 gaaaagatga gtataattag aggtacagtt aatcatattt ttatgattaa tgtacctcta
     2701 gttttttgta tggtagattt attaataaat atatcataga agtgttttga acatggaaat
     2761 atttatgtgc attctttaaa taaaagttta ttttttgaat gataattaag tgcttgttgg
     2821 taaggttatg gctgtaaaaa tcataacttt atttttatat ttgttccatt ttcataaaaa
     2881 ttattatccc tttaagaaaa tttcttaatc ctcttgaaat atttcctttt tagtctaaag
     2941 ttaaatttct ttcttcaaaa tgaatattta aatgaactaa aatttaagta cattttctaa
     3001 tcttatgagt ctatttttca tagataattg ccctataata gaagaatcta tgttggggct
     3061 atgtggtgac aataacagca ttgtcagtga ttaattaatg ctttataaat gattaattag
     3121 tgttttatgt tatttttgga cttaccctct atacatagat taatttattt aactttagat
     3181 atagagagaa tgtcaagtac atacacagac attagagttt aagaaagtgt ttatggagca
     3241 agaactttta aaagaagtag tgacttagtt taatttcaag aaatgataaa tacaaaagag
     3301 gaaaaagaga ggtgttgaaa tgatagaaaa agtagaagaa aaatctaaga ttattaaaac
     3361 atcaaaagaa tataagcgaa ctataattga agaaaaagat aaagaatgga ccaaaaaatt
     3421 aaatgatata cttagtagaa gtagtttttg taatgatgaa ttagataaaa tcaaggcgtt
     3481 aattccaaaa gagattttga ctaatgaaaa tgttggagaa gttgtgactg aagatggata
     3541 tgctaagccc gagtatgatg aggtatttaa atcattgttc actttaaata atgaatatga
     3601 tttacttgta aattttataa atgatattct aaaagatgct ccatatgcta acaggaatat
     3661 caaaccgttc actcaaataa agagaataat tagagttgaa actgatccaa caattaatta
     3721 cattggagag aaaagaccta gacttgatta aaaaatagct acgcacactt gtgactttag
     3781 tcatgagtta gtagctaaaa tagtcagcat atagggaaac ttatatgtag
//
LOCUS       DAELXX010000200         3821 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.202, whole genome shotgun sequence.
ACCESSION   DAELXX010000200 DAELXX010000000
VERSION     DAELXX010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.202"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..556
                     /locus_tag="KPH29_003597"
     CDS             <1..556
                     /locus_tag="KPH29_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HBG1469157.1"
                     /translation="ILISHINILIIADINNKDICLKLNIKYMFNLININRQLYPAENS
                     KNNDKKEGMKNNIDSSILLADDLLSIYRLLKKIKIHELYSNINFGTGNIGLTSSVYVL
                     INTLYGNLFNMIDAEKMYLNVNPDFTKDYVLGNIRIHIRPRIKALFNIIIMINKIMNK
                     NKGNKEGDSNESNRFDTKSYGNNS"
     gene            510..938
                     /gene="ytfJ"
                     /locus_tag="KPH29_003598"
     CDS             510..938
                     /gene="ytfJ"
                     /locus_tag="KPH29_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HBG1469158.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            1186..1626
                     /locus_tag="KPH29_003599"
     CDS             1186..1626
                     /locus_tag="KPH29_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG1469159.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDIDNKDKLFTSLEESKNIIEQLF"
     gene            1642..3093
                     /locus_tag="KPH29_003600"
     CDS             1642..3093
                     /locus_tag="KPH29_003600"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBG1469160.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVSTTFDKINMISRNFNS
                     KENSIDTVTLVENNNLVNVCLVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNVLNLNLNSDEIGSFVEGILLGNYKFDKYKTKSKDFERKEVDTITIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAIETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALTLLGNVATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(3184..>3821)
                     /locus_tag="KPH29_003601"
     CDS             complement(3184..>3821)
                     /locus_tag="KPH29_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HBG1469161.1"
                     /translation="FVSLFIFGLFLYYCPKLTKNILPYSYTVEKIICTLLVIIMALEQ
                     LLLISSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSLVCSVGELIFSK
                     NLGYEFPSLIYFIFIFSKCLIIYADIYMVDVRKFRVNRYALRDNLAICFIYFSFIFLL
                     NTFTNSQYYYGFLSHSTTAIFTFIFVTSIMYIPALLFNRDTFILEKKKKSK"
BASE COUNT         1597 a          352 c          657 g         1215 t
ORIGIN      
        1 gattttaata tctcatataa acatcttaat aatagctgat ataaacaaca aagatatttg
       61 tttaaagctt aatataaaat acatgtttaa tttaataaat ataaatagac aactttatcc
      121 agctgaaaat agtaaaaata atgataaaaa agaggggatg aagaataata tagattcaag
      181 tatattatta gctgatgacc ttttgagcat ttatagatta ttaaaaaaga taaaaataca
      241 tgaactttat tcaaatatta attttggaac tggaaatatt ggcttaacat cttctgtata
      301 tgtattgata aatactttgt atggaaattt atttaatatg attgatgcag aaaaaatgta
      361 tttaaatgta aatccagatt ttacaaaaga ttatgtttta ggaaatataa ggatacatat
      421 aaggccaaga ataaaagctt tatttaatat tataataatg attaataaaa ttatgaataa
      481 aaataaagga aataaggaag gtgatagtaa tgagagcaac aggttcgata caaagtctta
      541 tggaaacaac tcttgaaaca attaaaggtt ctatagatgc aaatactatt ataggagacc
      601 ctataaaaac agatactaca gtggttgtac ctatatcaaa ggttacaatt ggctttggta
      661 taggaggagg agaatactca aaaggatatg atgataaaga tagggaaatt gaattaaaaa
      721 atgaaaaatc agatacgaat tttgctggag gtagcgcagg ggctatatct gtacaaccag
      781 tagcatttgt agtagttgaa agtggagaga ctagaataat gagtttagac agtaatatca
      841 atctagtgga taatatatta tctataactc ctagagtatt ggagaaaatt caaaatatat
      901 cacaaagtaa taagaatcaa gataaaaaca atatgtaaga ataaaatatt tttaaatatg
      961 tatatagttt taagtaaagt agtggggtta gtataaatcc cactattttg ttgtgtactt
     1021 taaacattta tatctaataa gattttagtt taaaacaatt taaatagata ataaagttga
     1081 tatatagtat aattataaaa agaaaagaag tattatatta tagatatata aacgcatttg
     1141 agatataaga gggagttata aatcattagt tttgaggtga atgttatgag cgataaagaa
     1201 ttatctattt tgttgttaga aagtatgaat aattttcata atttaataaa gattattaat
     1261 aacgaaagat ataaaaaaga taaggttctt acagaaagac agttctttgc cttagtaaaa
     1321 ataagaaaac atgataaaat agagcttaaa aatctaagta gagatttaca tgtatctact
     1381 tctagtctgt gtatactact taataaattg gttgagcaag agtatgtata tagggaagaa
     1441 gatagtagag atagacgaaa tactttttat ggaataacta aaaatggaga aaagatattg
     1501 gataatgaaa tactaaagtt tgtatctata ataagtgata aaatggattg cttagatata
     1561 gataataaag ataaattatt tacttctttg gaagagtcta aaaatataat agaacaatta
     1621 ttttaaaaag gagaatttat tatgaatata aagtattcta aagagatatt aaaaagttta
     1681 gaaaatgcta taacagtagt agctctattt gagggagaag atgtttctac gacatttgat
     1741 aaaataaata tgataagcag aaattttaat tctaaagaaa atagtataga tactgtaaca
     1801 ttggttgaaa ataacaactt ggtaaatgtt tgtcttgttg gttttggtaa gagagagtta
     1861 ttgaatagag aaaaaattag aatcataggt ggtaatttgt gtaaaaaatt aaaaaatata
     1921 ttgaaaaatg aagcatataa gagtagtgat gttaatgtac ttaatcttaa tttaaatagt
     1981 gatgaaatag gttcttttgt agaagggata ttacttggaa attataagtt tgataagtat
     2041 aagacaaaaa gtaaggattt tgaaagaaaa gaagttgata ctataactat atttactgaa
     2101 aaagaagttg agtgtcaaat agaaaaggct aaaatattag ctaattcaac tataatagct
     2161 agaaatctag ttaatgaacc atcaaacgta atatatccaa aaacattagc aatagaaaca
     2221 gttaaacttg gtgcagagtt cgggtttaat gttgatgttt atgaagaaga aaaaataaag
     2281 gcattgggaa tggatgcttt tttcgccgtt tcaagaggtt caatcaataa accaagattc
     2341 atagtaatga gatactttgg agataaagaa agtacagata tattaggtct agtaggtaaa
     2401 ggattgactt atgatactgg tggatattct ttaaaatcaa atgcttctat gctagatatg
     2461 aagacagata tggctggtgc tgcgagtgtt ataggtgcta tgtgtgctat atctcaaagt
     2521 aaattaaaga aaaatgttat agcagtagtg gctgcatgtg agaatgcttt atcaggaggt
     2581 tcatacaaac ctggagatat aattagttct atggcaaaga aaactattga agttttaaat
     2641 acggatgccg aaggtagact tacattagct gatgcaatat actacattat aaacaatgaa
     2701 aaagtaacaa aggtagttga tgtagctaca ttaactggtg ctgctttaac attgttagga
     2761 aatgttgcaa ctcctatagt aacaaataat gatgattttt attgtgaatt agaaaaagct
     2821 gccactttat caggagaaag ggtatggaag atgccaattt atgatgaatt taaagatatg
     2881 ataaaaggag aagaagcaga ccttaaaaac actggtggta aaaatgctgg atgtataaca
     2941 gcaggagctt ttattggtga atttgttggg aatactcctt ggatacatat ggatatagcg
     3001 gggacaagta catcatcaaa atcaattgga tacaaagcaa agggggctac aggagaacct
     3061 gttagaacat tatactattt ggcagaaatc taaaaaacaa ttataaaata taatttataa
     3121 agccacatgg atatttttgt aatttaagat tatctatgtg gctttatttt ttatttaagt
     3181 aaattattta ctctttttct ttttctcaag tataaaagta tctctattaa ataaaagagc
     3241 aggtatgtac ataatactag taacaaaaat aaacgtaaaa atagcagttg tgctatgact
     3301 taaaaaacca taatagtatt gtgagttggt aaatgtgttt agtaaaaata tgaatgaaaa
     3361 ataaataaaa catatagcta agttatctct aagtgcatat ctgttaactc taaattttct
     3421 aacatcaacc atatatatat ctgcataaat tatcaagcac ttagagaaga taaatataaa
     3481 atatattaag ctaggaaatt catagcccaa gtttttagaa aaaatgagtt ctccaactga
     3541 acaaactaaa ctccaagaaa aaaatatatt aaaaagatga tactgcttaa aaattagtat
     3601 ggctatacac agatatatac agatataatt gattccaatt ggtaaagagt ttagagtact
     3661 ataattacct gaagaaataa gtaataattg ctctagagcc ataattatta caagtaatgt
     3721 acaaattatc ttctcaactg tatagctata aggtaaaata tttttggtta gttttggaca
     3781 atagtataaa aataatccaa atataaagag acttacaaat t
//
LOCUS       DAELXX010000201         3653 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.203, whole genome shotgun sequence.
ACCESSION   DAELXX010000201 DAELXX010000000
VERSION     DAELXX010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.203"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            120..749
                     /locus_tag="KPH29_003602"
     CDS             120..749
                     /locus_tag="KPH29_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1469162.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIIAECEPQLEYVK
                     KQYKEYTKKTENTMLDCRCPESISLLKRNNLINNFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
     gene            750..1532
                     /locus_tag="KPH29_003603"
     CDS             750..1532
                     /locus_tag="KPH29_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG1469163.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAINTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            1525..2403
                     /locus_tag="KPH29_003604"
     CDS             1525..2403
                     /locus_tag="KPH29_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1469164.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNEEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRIIGKIINDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIKIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKIKEFNIEDGYNRDLTSEYYI
                     KLKKDILMTMKSNKDI"
     gene            2436..3395
                     /locus_tag="KPH29_003605"
     CDS             2436..3395
                     /locus_tag="KPH29_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG1469165.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDVALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
BASE COUNT         1458 a          347 c          660 g         1188 t
ORIGIN      
        1 ttaaaaaatg tagataaaaa ttgttttatt aaggaaaaat taatatattg acaaaattta
       61 agtgctcatt taaactatta attgataata atattcaata ttaaataata ggaggcgtta
      121 tgaataaata tcttctaata aatccagtgg cagaaagaat gtatggagaa aagtttcacc
      181 ttattaaagc aaatttgata aaaaaggggt acataatagc agaatgtgaa ccacagttag
      241 aatatgtaaa aaagcagtac aaagagtata caaaaaagac tgaaaataca atgttggatt
      301 gtagatgtcc agaaagtata agtcttttaa aaagaaataa tttgataaat aattttgaag
      361 tacctatgat agaaccaata ttagtaagga cttgtagggt tttatacgat aaatatataa
      421 aaagtaaaga tgatatgttg gtagtaacct gtccatgtac acaattaaga gactttgcaa
      481 gtgaaaagtt taaggataag ggtaatgcta tgttttatac atggaaagag tttgctgaac
      541 aagaaggtgt aaaatcactt ggaaaaatag atgaatcacc aattccatta ggatatttca
      601 aagatacttt tgaaaaagtt cttgaagtat caagtgaaga cgaaatatta agtgaaatac
      661 aaaaaataag aaatggcttt gaagataagt atgacatagt agaaatgttt tattgtaaag
      721 atggttgtaa taatggagat ggtttgtaaa tggtcaataa atttaataat aagtttttaa
      781 ggctagtaaa gagtgttctg atatttttta taattctttt attatggaaa attacaaatt
      841 atcttggtat ttggagtgat tatatattac catcacccga aaaggtatat agtacatttt
      901 taaatatgat aagtgatggt tcaatattta ttaatgtata tgcaagtatg aaaagagttt
      961 tgataggttt tgcaataaat actgctatag gtgtgccttt agggatattc tttggaattt
     1021 atagtggagt atacgaatac tttaaatctc taataaattt tttaaggaat acacctcccc
     1081 ttgctttaat accaatgctt atattatggt ttggtatagg agaggaatca aaaattataa
     1141 ttatagtttt agcatccttt ttcccaatct ttacaagcac attgaaaggg attaaaaatt
     1201 gtgactcaaa actgattgaa gtgggtagag tatttgagtt ttcaaagctt cagataatat
     1261 ttaaaataat aataccaaat gccattcttg atattgctgt tgggttaaag ttagctcttg
     1321 gatatagctt tagagcaatt attggggcag aacttgtagc agcttcatct ggtcttggat
     1381 acttgatatc tgatggaaaa gaaatgtcta gaacagatgt tgttattgta ggtataatcg
     1441 taattggact tttaggaata attacagatt acatattttc gattattgtg aagaaggtaa
     1501 gtaagggaaa gatggtggaa gcatatgaat aatttagtat gtgactctgg atatataatt
     1561 agaaatttat ataaaagtta taaagttgac aatgaagaac atgtagttct tagtgatatc
     1621 tctttaaaca tagatagaaa ccatataaca gtaattttag gtgaaagtgg ttgtggaaag
     1681 actaccttgc ttagagcaat tgcatcactt gaaggtataa atagtggtga gattaggttt
     1741 attaatgata ataaagagta tagacctaat gttggttttg tttttcagga gagcagatta
     1801 atgccttggt taaacgtaag tgaaaatata gcttttcata atcaaagaaa aaatactatt
     1861 ttaaacaagg tgcttagaaa atttaaacac aataaaagaa ttattggtaa aattataaat
     1921 gataatttta gaagcttaga aacaaatgat aatttgaaca aatcaaataa atataatagg
     1981 aaaagtaata ttgatgtaga aaaatatctt aagatgatga acttgcagaa atttaaaaac
     2041 tcttatccaa atgaattgtc aggaggtatg gcacagagag taagcatagc tagagctctt
     2101 tctttcaatc cagatatgtt gcttatggac gagccttttt cggcattaga ttattttaca
     2161 aggattgata tgcaaaatga agttataaaa atacataagt caactaataa aggcgtaata
     2221 tttgtaactc atgatattga tgaagcgtta aagataggca aaaaaatagt tgttttcaca
     2281 gacgaaagaa aaataaaaga atttaatata gaagatggtt ataatagaga tttaacttca
     2341 gagtactata ttaaattaaa aaaagatatt ttaatgacaa tgaaatcaaa taaagacatt
     2401 taaagaaatt aataatattt taaggagtag agtaaatgaa attaaaaaag ctattgatag
     2461 gtataacgat attaacactt gcaacagcat ctttagctgg atgtgctaaa aaaaataatg
     2521 gagaaaaact ttcagaaata aatttaactt atgtaaaatc accattaaat gttccttcta
     2581 taattcagaa acaggatgat ttatttggaa aggaatttaa aaaggataat ataaaagtta
     2641 actttcacga aattactaca ggtccagagc aaacacaggc tttagctgct ggagaaatag
     2701 acttcttaca tgcactaggt ggtacgtcag ctttaatagc tgcatctaat ggagtagagt
     2761 taaagatatt aaacacttat agcaggtcac caaaaggatt tatgattttg actaataata
     2821 attcaataaa atcagcagca gatttagtag gtaagaaggt ggcaggtcca aaagggacta
     2881 tattacatca ggtattgatt tcagcacttg ataaagaagg attaagtatg gatgatgtgg
     2941 aatttgttaa tatgggtatt ccagaagcat cagcagctct ttctgatggt agcgtagatg
     3001 ttgcattgat tgctggtcca gcagctctta aagctatgaa gtcaggtagt aaacttgtag
     3061 caaatggtga aggtttagta gatggtataa tagtaactgc tgttagcaca gactttgcag
     3121 agaaacaccc agaaatagtt gaaagattta tgaaggttga aaaggaaact ttagaatatg
     3181 taaataataa ttttgatgaa gctatggaaa aagtagctaa agaagttgat ttaagtcttg
     3241 aagaaactaa agaattatat gcttggtatg attttagttt agatataaca gataaagata
     3301 tatcttcgct tgaagataca caagacttct taataaaaaa taagttacaa gagaaaaaag
     3361 tgaatataaa agaattaatc tataatgcta agtagaaact atgctaaaat aatactaagt
     3421 aagaaatata ataaaagttt aagttacctt attaaggtaa ctttttctat tttgaattag
     3481 taatttcaat ttattttaga atgattagtt gataataatg attaattaat cataaaaaga
     3541 tactttttta tagagtttta ttaagatgtc taataatatg gttatactta tgaagtcagt
     3601 aattgtgaag ttaataatat gattaaattt atatattgta aaaagattat aat
//
LOCUS       DAELXX010000202         3625 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.204, whole genome shotgun sequence.
ACCESSION   DAELXX010000202 DAELXX010000000
VERSION     DAELXX010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.204"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..873
                     /locus_tag="KPH29_003606"
     CDS             <1..873
                     /locus_tag="KPH29_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG1469166.1"
                     /translation="LFLFNYTIVYEVKEPKENAVGNNITQIDISTDNNLMLYVAYLWL
                     VVTIVIAVYTFIKYTRFKNLVVDVSYDIEDNDVNCIYKNLLKELNIKKKIELRGSDEL
                     ISPAGMGLFKSYIFLPDYPYSKDELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNP
                     LVYVMSNKVNLDCELCCDESVLTDCSLKDKKEYALALIKSIKLSKNYNSGILTTEFNK
                     TSLEKRLDSIVKKKGKNGILVLLILITLFSVTYFNFESISFDNSNSLRNMGILQKSGV
                     DLKEEDDISMRVIN"
     gene            939..1526
                     /locus_tag="KPH29_003607"
     CDS             939..1526
                     /locus_tag="KPH29_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HBG1469167.1"
                     /translation="MKNLKKILIITGCLTLISTSLVFANTTTDVDTSKDDDSIISSCT
                     ITGYEFTYENAPQSVKFDYEKKCEALNIKPSKNDIIFVNKDSIDANDVENYVEPKAYI
                     IRYKKGYVFEVTGPKSYTLNVSNTVVGYNRVESGNPVHLVQLLCRTLDGSSIDDDSEF
                     GPATYNAVKSLQGRLGLTRDGIVGRSTWDAAGRRL"
     gene            complement(1904..2665)
                     /locus_tag="KPH29_003608"
                     /pseudo
     CDS             complement(1904..2665)
                     /locus_tag="KPH29_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612258.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3273..>3625
                     /locus_tag="KPH29_003609"
     CDS             3273..>3625
                     /locus_tag="KPH29_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG1469168.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDATVTSKDVIEVMEQKYGWKQTTT
                     LTLLSRLVKRGFLNAQKIDRYTHYTVIVKHKDYLSFETKDFLSNIHDDSLKSLISALH
                     DDENIGKDTLDFLES"
BASE COUNT         1367 a          396 c          507 g         1355 t
ORIGIN      
        1 ctttttctat ttaattatac aatagtgtat gaagtaaaag aacccaaaga aaatgcagta
       61 ggaaataata ttacacaaat tgatatttct acagataata atcttatgtt gtatgtagct
      121 tatttatggt tagttgtaac tatagtcatt gcagtttata catttataaa gtatacaaga
      181 tttaaaaatc tagttgttga tgtatcttat gatattgaag ataatgatgt aaactgtatc
      241 tacaaaaact tgttgaaaga attaaatata aaaaagaaaa ttgaattaag aggttctgat
      301 gaattgataa gcccagcagg catgggatta tttaaatctt acatattctt accagactat
      361 ccttatagta aagatgaact aacttggatt ttgaaacatg aattaatgca ttttaaaaat
      421 aaagatattt taataaagtt tttagtgtta tctgttagaa taatatattg gtttaatcca
      481 cttgtttatg taatgagcaa taaagtaaat ctagattgtg aattatgttg tgatgaaagt
      541 gttttgactg attgttcttt aaaggataag aaggagtatg cattagcact tataaagtca
      601 attaaactta gtaagaatta taatagtggt attttaacaa cagagtttaa taagacaagt
      661 ctggaaaaga gacttgacag tattgttaag aaaaaaggta aaaatggaat attggtgtta
      721 ttgattttga ttacattatt ttcagttaca tattttaatt ttgaaagtat tagttttgat
      781 aattcaaatt ctttaaggaa tatggggatt ttacaaaaat caggagtaga tcttaaggaa
      841 gaagatgata ttagcatgag agttataaat taatagatta atgtatgtaa tgaaaaggta
      901 tattgtcgta ttattaataa attaggagga attatgaaat gaaaaattta aaaaaaattt
      961 taataattac aggttgtcta actctaatat ctactagttt ggtatttgca aatactacta
     1021 ctgatgtaga tacatcaaag gatgatgatt caataataag ttcatgtact ataactggtt
     1081 atgaatttac atatgaaaac gcaccacaat ctgtaaaatt tgattatgaa aaaaaatgtg
     1141 aagcgctaaa tataaaacca tctaaaaatg atataatttt tgttaacaaa gattctatag
     1201 atgcaaatga tgtagaaaac tatgttgagc caaaagcata tattattaga tataaaaaag
     1261 gttatgtttt tgaagttact ggacctaaga gttatacatt aaatgtaagt aatactgtag
     1321 tcggatataa tcgtgttgaa tcaggtaatc ctgtgcattt agttcaactt ttatgtagaa
     1381 ccttagatgg ttcaagtatt gatgatgata gtgaatttgg acctgctaca tataatgcag
     1441 ttaagtcact tcaaggaaga cttggattaa caagagatgg aattgttgga agaagtactt
     1501 gggatgcagc tggaagacgt ctttaaaaag aaagctattg cacagataat tttaaattgc
     1561 tgaatctgta aacatttaca tgtattcttt aaataaaaat ttatttttta agtgataatt
     1621 aaatttttac taatagggct atggctttaa aaatcatagc cttattttga tatcttttcc
     1681 atttttgcaa aagctattaa ccctttaaga aaatttcttg tttttttgaa aaatttcttt
     1741 cttcaaaata aatacttaaa tgagctaaaa tttgagcaca atttctaatt ttctgagtcc
     1801 atttttaaga aatatatatg gttgttaagt atagtgattt gtgcaaataa ttatccaaag
     1861 taaaaataga tttcaattta gtaacttttc ttaataatct attctaactt cccattgaat
     1921 ttgtagtata tatcgatgta tttatttctt gagagtactt aaaatatgtt actaattcat
     1981 tccaattgtt tcaccaacct cttaatacta ttgtatattg attgtactat tttttctcta
     2041 gtgatagctc acttaatgct acctcttctg taattgtagc atatctttct ttaaatcttt
     2101 tacagattct tttctatttt tatatgatac ataatttaaa gtatttttaa tttgatgcac
     2161 aacatatctt tgtacttaag tatcaggaaa tatcgcttta attgcattag aaaattcaac
     2221 tagtctatct atagaagctg ctattaatac atctttaatt cccatgtttc tagcatctat
     2281 aagcactttt agccaatact ttgaaatttc attttctcca atccatattc ttaaaacctc
     2341 ttttatgcta catatagcta tatagactgt tttgtttatt attcgacctc acttctaact
     2401 ttataatgta tagcatctat aaatacaatt gaataaatta tattaagagg tctaagttac
     2461 catttatgag ttaaaggaat aactttatca gtagttttag ttattagtga atgagataat
     2521 atcaattcca taagtacttt taagatgact taatatattt tgagtagaca tgccttttga
     2581 gtatattcca atcacttgat tttttattcc taatatatca ttttctatca taacctaaaa
     2641 gttcattaag ctccgcttct aacatttaat ggattgtact ttcaaataaa tcttttagta
     2701 tatcttgaat atctgtaatt gttttggcat catattcttc aataaatttt tttaatattt
     2761 tagtttttgt taaacataaa aagtgaacat cttcctttgt atagtagtga tattttaatt
     2821 atcacaactt tttagaagaa tacaaacttt attttaaaga cactgaaaat ggtgttttta
     2881 tacctttatt tgctatgtat aattttactt ataacataaa cttatataaa acagatattt
     2941 taataacaca aaggtacaat taggagattg ttaaaattta ttagatatta ctctagttta
     3001 tttaaaaaaa agtattataa tattccttgt gtgaaaatat aggaattttt attaaataat
     3061 tttaatgtgg aaataacatt ctgcactatt ataattaaat atgtaaaaag caaaatactt
     3121 gaacaactat attttaaaat ctaactttta gtattttaaa aaattagaaa attaataaaa
     3181 agtacaaaaa tccacatttt ttcataaagc gtttgactaa aaaattcaca tattgtagac
     3241 ttaaagtagc aagagttttg agggaggtgt aaatgatgat aagaaaaata ccacaagcag
     3301 aactaaaagt aatgaaattt atttggaaag tagatgctac agtgacatca aaagatgtta
     3361 ttgaagttat ggaacaaaaa tatggttgga aacaaaccac tacactaaca cttttatcga
     3421 ggttagtaaa aagaggattc ttaaatgctc aaaaaataga cagatataca cattacacag
     3481 taatagtaaa acacaaagat tatttaagtt ttgagacaaa ggatttccta agtaatatac
     3541 atgatgattc tttgaaaagc ttaatttcag cattacatga tgatgaaaat ataggtaaag
     3601 acacgctaga ttttttagaa agtta
//
LOCUS       DAELXX010000203         3519 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.205, whole genome shotgun sequence.
ACCESSION   DAELXX010000203 DAELXX010000000
VERSION     DAELXX010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.205"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            128..463
                     /locus_tag="KPH29_003610"
     CDS             128..463
                     /locus_tag="KPH29_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1469169.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            600..1877
                     /locus_tag="KPH29_003611"
     CDS             600..1877
                     /locus_tag="KPH29_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HBG1469170.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEENKRENSTDKEENSDGNNSQKDSNNDNNDNG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKDQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            1998..3386
                     /locus_tag="KPH29_003612"
     CDS             1998..3386
                     /locus_tag="KPH29_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG1469171.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRVLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSESAIVEYEVDGKSKELTSNMILVATGRKANTEGL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIVINQLFGD
                     KTRTTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPNMIKEV"
BASE COUNT         1450 a          339 c          610 g         1120 t
ORIGIN      
        1 agtttgtaat aggagtatat ataataaaaa ttttaattaa ataaagctag gtatatttat
       61 aatattaata aatatattgc tataaaagtt tgtttaataa attaaataaa gaaaagagga
      121 gaataaaatg tacaacatag atgatatgat atataattgt ccagtagaag ctttatcaaa
      181 tattttaggt aaaaaatggg tggctataat tatatggaaa atacaagaag ataagaaacg
      241 atttggagag ctacaaagag ctataccaga ctgtagtaag aaaatgttag ttcaacaact
      301 agatttttta attgaacagg ggattatagt caaccaaaaa aagttggtta ataatgttgt
      361 agagtcaaca tattttttaa gtgaatcagg attacgattg cttcctgtca tggaaaaaat
      421 gattatgtgg agcaataaaa atttaaattg tgataaatac taaaatttac tctgtaagta
      481 atgaaaattt aattaagcta tgagtagaaa aatgtattaa aattacataa ttttttagtt
      541 agattttaat ttagaaatgg gattgtaata ttggaaataa aataaggagg aattttaatt
      601 tgcaaaatgt aaatttattt ttagtattta tagaaggtat agtatcattc ttttcaccat
      661 gtatactgcc aattttgcct atttatttga gtatattgtc aaatagtagt gtagaaaatc
      721 taaaagaagg aaagacgagt tttataggaa gttctttatt taaaaatact atattttttg
      781 ctcttggaat ctcaacaaca ttctttatat tgggttcatc agtaaaagta ttgagtatgt
      841 tttttaatga aaataaagat ttaataatgt ttattggtgg gattataatt attatcatgg
      901 gattatttta catgggaata attaaatctt caatattgaa tagagaaaaa agatttaatg
      961 ttaagtttaa agaaatgaaa gcaataacag cttttatatt aggttttacg tttagttttg
     1021 gatggacacc ttgtattggt ccaatattgg catctgtatt agtcatggtt tcaagttcaa
     1081 gtaatcatct tagtgccaat cttttaatag cagtatatac tattggtttt attttgccat
     1141 ttataattac agctatgttt tacagtaaac tatttaaaac tatagataag ataaaatcta
     1201 acatggaaat tattaaaaaa ataggtggga ttatacttat agtatcagga atattaatga
     1261 tggtaaatgg atttggaagt ataagcaaac actttaatac gtcacaaaat agcaaaattg
     1321 aaagcaagca agaagaaaat aagcgagaaa attctactga taaagaagaa aattctgatg
     1381 gtaataatag tcaaaaagac tctaataatg acaataatga taacgggtct aatgatgaag
     1441 atagaataaa gtctattgat tttactttaa cagaccagta tggtaaaaca cataaattaa
     1501 gtgattatga aggcaaagtt gtattcttaa atttctgggc tacatggtgt cctccatgta
     1561 aagaagaaat gccttatata gaacaactgt ataaagacta taataaaaac aatgatgatg
     1621 tagtaatttt aggagtagct tcacctaatt taggtagaga aggttcaagg gaacatgtag
     1681 ttaacttctt aaaagaccaa ggttatacat tccctgttgt acttgatgaa gatggagcat
     1741 tagcatatca atatggaatt aatgcttttc caactacatt tataattgac aaggaaggat
     1801 atgtaactca gtatattcca ggagctatgg ataaggcaac tatggcatca tttattgaaa
     1861 atcagagaaa taaataaaag tatttaagta tatgtttatg aatcaattta aggttacaaa
     1921 aaagtgccta attgtaccac ctaaataatc ttgttatagt aaaagtataa acaatacaag
     1981 aaagggttga attttatatg aaaaaaacat ttgatgctat aataatagga tttggaaaag
     2041 gtggaaaaac attagcagga gatttggcaa acaggggatt aaaagtagct ttaatagaaa
     2101 agtcaaacaa gatgtatggt gggacatgtg taaatgtggc atgtatacca acaaaaagct
     2161 tagaaaattc agcaaacagc gtaaaaacaa agaatataaa ttcttgggat gaagtacaag
     2221 ctgaatatga gaaagctata gataaaaaag aaactttaat tacaaaactc agagaagcta
     2281 actataataa attaaattca aatgagaatg taactatatt cacaggaatg ggaactttca
     2341 ttgatgaaaa aacagttcaa gttaaaactg aaaatgaaat ttatgagtta gtagcagata
     2401 atatatttat taatactggt tcaagaccat ttatacctaa tattaaggga attgaaaata
     2461 agaatatagt atatgatagt gagtctctta tgaatctaag agttttacct aaaaaaatga
     2521 caattatagg agctggattt ataggtttag agtttgctgg tatttatagt tcatttggag
     2581 cagaagttac aatattaaac tctaataatg gcattttacc aaatgaagat gttgaggatt
     2641 cagaagaaat aataaaatta cttgcaaaga gaaatgtaaa aattgttaat aatgcaaata
     2701 taaaagagat taaagaagtt tctgaatcag caatagtaga atatgaagta gatggtaaat
     2761 ctaaagaatt aacaagtaat atgatacttg tagctacagg aagaaaagca aacactgaag
     2821 ggctagggct agaaaatgca ggtatagaat taaatgaaag aggatttatt aaagtctcag
     2881 aaactcttaa gactaataaa gagcatatat gggctatagg agatataaat ggaggtccac
     2941 aatttactta tatatcattg gatgattatc gcatagttat taatcagtta tttggagata
     3001 agactagaac aactaatgat agaaagaata tcccaaattc tatatttata agtccagcat
     3061 tttcaagagt tggtcttaat gttaaacaag ctaaagaaaa gggatatgaa gtattagtgg
     3121 caaagatgcc agtagaagca attccaagag ctaaacagat tggcaaggca gatgggttta
     3181 taaaaatagt gatagataaa aaatccaata agatacttgg agctagtatg atatgtgaaa
     3241 attcaagtga gattatccat cttattcaat tagctgtaga tttagaagta gagtatacat
     3301 atttaagaga ccgtgtttat gcacatccaa ctatgacaga agctcttaat gatattctat
     3361 ctcctaatat gataaaagaa gtataacata agtttaatat ataaacctaa aattatcatt
     3421 ataagtagat tttagaaaga ggctatctca aaaaatagag ataagtctct tttttacttt
     3481 ttaaatgata aaaatatatt tgattttata aaagaagat
//
LOCUS       DAELXX010000204         3455 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.206, whole genome shotgun sequence.
ACCESSION   DAELXX010000204 DAELXX010000000
VERSION     DAELXX010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.206"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            226..1824
                     /locus_tag="KPH29_003613"
     CDS             226..1824
                     /locus_tag="KPH29_003613"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HBG1469172.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDMDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILAVTDIIKEGSYLIYKAEDSLIANAFDVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
     gene            complement(2059..2250)
                     /locus_tag="KPH29_003614"
     CDS             complement(2059..2250)
                     /locus_tag="KPH29_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium protein"
                     /protein_id="HBG1469173.1"
                     /translation="MAGLTTEPGGGGVLSGYFAGFLFGGTTFTINNFSSTTVGIRNGQ
                     SAGTAATLTIFRIADTVMT"
     gene            complement(2419..3012)
                     /locus_tag="KPH29_003615"
     CDS             complement(2419..3012)
                     /locus_tag="KPH29_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HBG1469174.1"
                     /translation="MRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYY
                     NLRTITWNDRVSMENIRGYLPIGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNY
                     PYQIFEFTSSRDCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSIGPMG
                     ATGPTGATGNSSQPIANFLVNAPSPQTLNNGNAITGW"
     gene            complement(3013..3159)
                     /locus_tag="KPH29_003616"
     CDS             complement(3013..3159)
                     /locus_tag="KPH29_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium protein"
                     /protein_id="HBG1469175.1"
                     /translation="MRKIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFF"
BASE COUNT         1247 a          473 c          467 g         1268 t
ORIGIN      
        1 aatcaattta ttttaaataa aagtaaattt gattctatgt ttggttgggt aaaaatatat
       61 ataataataa tttttatatt aaatttgtag tatttatgta agaaataaat tttatataat
      121 attttttcac taaatttttt tctttttaca acaaatactc actacctttt gatataatat
      181 ttttatcaat tattattata ttcaagtttt gaaaggagta ttaaaatgag tttattagtt
      241 ttcggacata aaaatccaga tacagattca atatgttctg caatttcatt agcacattta
      301 aagaacaaac taggaataaa agcaacagct tatgctttag gagatatcag aaaagaagct
      361 aaatatgcct tggattactt taaggtagat gcacctgaaa ttttagataa tgttagaata
      421 caagttagag atttaaatta tgataaagta gtacctttaa caccaacttc atctatatta
      481 gaggcttata accttatgga tgaaaaaaat gttaaaacac tacctgtaac ttatgatgat
      541 ggaacttttg ctggtatcat tactatgaaa gaaatagcta aaaatctact tcatcaacac
      601 tttacaacta tacacacatc actatcaaac atttgtaaaa accttaatgg tactattctt
      661 gtggactgtg gtgaagatat aaaaggtaga gtagtcactt tatcatttgg aatggagact
      721 ctaatagaga ctttaaaaga aggagacata gctattgttg gagatagata tgatagtatt
      781 gaatatgcta ttgatacaaa agttaaactt ttaatactta ctaatcatac agagatttca
      841 aaagatttgc tagctcttgc taagataaat ggagtgtctg ttgtttctgt tgaaagtgac
      901 aattataaaa cttctaatgt aataaatcaa tgtagtttct tagactctat agaagctaca
      961 gaaaatttaa ttacattcaa agaagatgat tatatggatg aaataaaaga gattatgcta
     1021 gaaacaaact tcagatctta tccaatatta gatgatgata ataagttttt aggtttagtt
     1081 tcaaaaggtc atctattaaa cccatctaaa aagaatgttg ttttagttga ccataacgaa
     1141 tatgctcaaa gtgctgatgg aattgaacaa gcaaacatag ttgaaataat tgaccatcat
     1201 aaacttggtg gtatttctac tgatgttcct atgtcattta gagttatgcc tgttggatgc
     1261 aatagtacta taatttatca aatgtacaaa gaaaacaatg ttgagatacc ttatgaaata
     1321 gctgggcttc ttttatctgc tatattatca gatacattat tatttaagtc tccaacaaca
     1381 actgatatgg ataagaaagc ttgtgaagaa ttaagtaaaa tagcaaaagt agacatggaa
     1441 aaatacgcta tggatatgtt taaatgcggt acttctttag atgaatatac tatagaagaa
     1501 atagtaaata tggattttaa agaatttaat atgagtggac atagagttgg tattggacaa
     1561 gtatttacct tagacataga ctctatattt gctaaaaaag atgagttttt atcttatata
     1621 aattcgactg attatgacaa actaattctt gcggttacag atataatcaa agaaggttct
     1681 tatttaatct ataaagcaga agatagctta attgctaacg catttgatgt tgaaggtgtt
     1741 caaggtacat ttgcaccagg tgttgtatca agaaaaaaac aattagtccc taatttaact
     1801 actgcaatca aaaattttaa ataatataaa atttttaaat aacgaaaact gtctcaaatc
     1861 tatatttgag acagtttttt attatacata tattgataat aaagttaaat aaaaatccat
     1921 aaatttaaaa tttttatgaa tatattttat tattattgtt attttattca ttctaaaata
     1981 ggtaaaactc aatatataaa aatacttttg tcatttaaat aaaacctaaa tagtttttaa
     2041 gattatttta gacacgtttt aagtcataac agtatcagct attctaaata tcgtcaaagt
     2101 agccgcagtt cctgctgatt gcccatttcg tatccctact gttgtagatg aaaaattatt
     2161 tattgtaaaa gtagtccccc caaataaaaa accagcaaaa tatccagaaa gtactcctcc
     2221 gcctcctggc tcagtagtaa gccctgccaa atcttttcct cctaaaattg ggaataggat
     2281 agcaaaagaa tattgattta tacttgatga acctggttgt aatgctactg aaactgatat
     2341 ataatacaca ccattttctt gtactgtaaa cgtaccattt gcatctactg ttatacttga
     2401 actatttcct attattgttt accaacctgt tatagcattt ccattattta gtgtttgtgg
     2461 agatggtgca tttacgagga agttagcaat tggctgagag gaattgcctg ttgctcctgt
     2521 tggacctgtt gctcccattg gtcctatact tcctgtcgct cctcttggtc ccattggacc
     2581 tgtaattcta attggaagac actcacattt aggataacaa ttatctataa ttctatcttc
     2641 aaatgttaca agtatatacg gtttgttaca atccctacta gatgtaaatt caaaaatctg
     2701 ataagggtaa tttaaagata aagctaaccc ataattagga tatttattca ttgcccatgc
     2761 ttttatagtt cccgtaatat ttaaacaaat atagttggat gtatcactta tcccaattgg
     2821 taaatatccc ctgatatttt ccatagacac tctatcattc catgttatag ttcgtaaatt
     2881 ataatattca cttattcttt taatttcaat atctactttt tctttaaaaa tatgattctt
     2941 atttgaatca atatgaataa ataactctgc ttttaatata cttttgtctt ttaatatatc
     3001 tggtattctc acttaaaaaa aagtaatcaa cttttctttt acaaaactat ttgaacattt
     3061 acttcctatt aagcaataat ctaaattact aaagttttta tctggatatt ttttagatat
     3121 aaaagtatca tcatttaaat aaagtataat ttttctcatt tataatccct ccattttggg
     3181 tctatatcat actatgttta aaatacatgc ttagttacaa tacaattaaa atttatagta
     3241 ttaaattttt atataataat gctgatttct acaaaaaaag aacatgtcat tagaaacttc
     3301 taatagcatg ttctttttca ttattatata ttacacttta aattattcct tgactggctt
     3361 agctggtgta aactaaacca gtctttctaa aatattaagc acttttttct tattatgttt
     3421 tttaggattt attgtctcta ttattgtaaa ccaaa
//
LOCUS       DAELXX010000205         3396 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.207, whole genome shotgun sequence.
ACCESSION   DAELXX010000205 DAELXX010000000
VERSION     DAELXX010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.207"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1123
                     /locus_tag="KPH29_003617"
     CDS             <1..1123
                     /locus_tag="KPH29_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1469176.1"
                     /translation="NQDLLNAFWKKGLSLNKFWIEENSLNNIDKKSINKIYNQGKTKY
                     SLLVKFIKKDNYLFAISIPIEHSEETIGIVNRFNIIMGIFSVVIITILTFILSNRVIK
                     PMERLKFLSKDISELNFRTEDIKTNDEIEELAYSINIMSEKLEKAHNELNKRNENLKT
                     FISDASHEMKTPIALIKAYAIGMKDGLDDGTYTDTIIEQAENMTNTINSLLYWAKYEK
                     KERSLCLVDLKERLYKSLKNYELLIERGSISVKCSVDDKELIISSDEDSIDMVFNNLI
                     SNAIKYTNNNEIEINLFEENERIILSIKNGIDYNMGDDIENMWKPFYVLEKSRSKELS
                     GTGLGLTIVRTILEENNFRYRVEICDGIIEFYIIFIKVI"
     gene            1548..>3396
                     /locus_tag="KPH29_003618"
     CDS             1548..>3396
                     /locus_tag="KPH29_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG1469177.1"
                     /translation="MKIPKKIAAMLTVTMIAGSSTAGIASAQTVATNLTGQERYETAV
                     KISQDGWKNADEVVIVNDSSIADALSATPFAKAKNAPILLTSKDKLNDKTKAEIQRLK
                     AKKVYLIGGTSVLSTNIEKEIKDLKISFERISGAERYQTSLELAKKLDAISDVKKIAV
                     VNGEKGLADAVSVGAPAAQNNMPVILADSKNGTAVADKFIKDAGITQSYVVGGESSIS
                     EAVKNKLPNSTRLGGTDRNDTNAKVIKEFYKKTDLKNAYVTKDGMNKQDQLIDALAVG
                     VLGAKNQSPVVLVGKNLSASQKSLVNSKSFDKITKVGGNGNETAFNEMKSLQEVKTVE
                     AKTISELKSAIDKATANDVINFKPTSEVKEAFTIQTDKAITVNLNGTYTKTVTINMPN
                     GDVNNYAKVDDVVIDDVKDGTFVNYGKITNLKVNDKNGAKIENNSKGEIGSLTVASGA
                     SQVKVTNGGKITTVTNNSKGTTIDNKGTISSVKGDNSPTISGNSPSSNSSGGSSSSGG
                     SSHGGGSSSSKVDKVVLKNTITAANKLYNEAIEGTNVGEYKVGSKAIYKTAIDKAQAI
                     LDKSGVTQKEVNDAVTALNTATDTFKAGKVVAVDKTALQDAVTAATALHA"
BASE COUNT         1470 a          364 c          598 g          964 t
ORIGIN      
        1 aaatcaagat ttattaaatg cattttggaa aaaaggttta agtttaaata agttttggat
       61 tgaagaaaat agtttaaata atattgataa gaaaagtata aataagatat acaatcaagg
      121 taaaacaaaa tatagtttgc ttgtaaaatt tatcaagaag gataattatt tgtttgctat
      181 atctatacct atagaacatt ctgaagaaac tattggcatt gtaaatagat ttaatataat
      241 aatgggaatt ttttcagtag ttataataac tatacttaca tttatattat caaacagagt
      301 tataaaacct atggaaaggt taaagttttt atctaaagat atatctgaat taaattttag
      361 gactgaagat ataaaaacga acgatgaaat agaagagcta gcttacagca taaacataat
      421 gagcgaaaaa cttgaaaaag cacataatga attgaataaa agaaatgaga atctaaaaac
      481 ttttatatca gatgcatctc atgaaatgaa aactccaata gcacttataa aagcatatgc
      541 aataggtatg aaagatggtt tggatgatgg gacttataca gatacaataa ttgaacaagc
      601 agaaaatatg accaatacaa taaattctct tttatattgg gcaaaatatg agaagaagga
      661 gagaagtctg tgtctggtag atttaaagga aaggttatat aaaagtttaa aaaactatga
      721 gctgttgata gaaagaggta gtataagtgt taaatgcagt gtagatgata aagaacttat
      781 aataagttcg gatgaagata gtatagatat ggtatttaat aatttgatta gtaatgctat
      841 aaagtatact aataataatg aaatagagat taatcttttt gaagagaatg agagaattat
      901 attatccata aaaaatggta tagattacaa tatgggagat gatatagaaa atatgtggaa
      961 gcctttttat gttttagaga agtcgagaag taaggagctt tctggtactg gtcttggtct
     1021 taccatagtt agaactatac ttgaagaaaa taattttaga tacagggttg aaatttgcga
     1081 tggaattata gaattttata ttatctttat aaaagtaatt tgaattaaaa tgtaaatata
     1141 aagaataatt aaaaaaatat cgcaattatt tgtttttcat atggataaaa ttggttattt
     1201 aatatataat tacaagtata aataataaaa tttatttgta aaaagagtag cacttttaat
     1261 tctaaaggct acttttttta tacaaaacca tgttttttat aacaattcat taacataaaa
     1321 atcaaactta ccttaaaata taaatttaca aatttttcaa tcaataagaa aatttctcta
     1381 ttccttacat aatgactgta atgtaacaaa ataattacaa attaattatg aaatcattat
     1441 agttagaaac ctctttaaaa ttagatatta agtatccttt agaattagaa cgggaacaaa
     1501 gttaaaaaat taagtaatat caaatggaca agggggaaga aaataaaatg aaaataccaa
     1561 agaagattgc tgcaatgctt acagttacaa tgatagctgg aagttcaaca gcaggcatag
     1621 ctagtgcaca aactgtggca acaaatttaa cagggcaaga aagatatgaa actgctgtta
     1681 aaataagtca agatggatgg aaaaatgctg atgaggtagt aatcgtaaac gactcatcta
     1741 tagcagatgc tctatctgca acaccatttg caaaagcaaa aaatgctcca atacttttaa
     1801 caagtaaaga taaattaaat gataagacga aagcagaaat acaaagatta aaagctaaaa
     1861 aagtatactt aataggtgga acttctgtat taagtacaaa tatagaaaaa gaaataaaag
     1921 atttaaaaat atcttttgag agaatctctg gagctgagag atatcaaaca tccttagaat
     1981 tagcaaagaa attagatgct ataagtgatg ttaagaaaat agctgtagtt aatggtgaaa
     2041 aaggacttgc agatgctgta agtgtaggag caccagctgc acaaaacaat atgccagtaa
     2101 tacttgctga ttctaaaaat ggaacagcag ttgcagacaa atttataaaa gatgctggaa
     2161 taactcaatc ttatgtagta ggtggagaga gttcaatttc tgaagctgtt aaaaataaac
     2221 taccaaatag tacaagacta ggtggaacag atagaaatga tacaaatgca aaagtaataa
     2281 aagaatttta taaaaagaca gatttaaaaa atgcttatgt aactaaagat ggtatgaata
     2341 aacaagacca acttatagat gcactagcag taggtgtatt aggtgctaaa aatcaatcac
     2401 cagtagtttt agttggaaag aacttatcag cttcacaaaa atctctagtt aactctaaga
     2461 gttttgataa gataactaaa gttggtggga atggtaatga gacagcattt aatgaaatga
     2521 agtcattaca agaagtaaaa acagttgaag ctaaaacaat ttctgaatta aaatcagcta
     2581 tagataaagc tactgctaat gatgttataa actttaagcc aactagtgag gttaaagaag
     2641 catttacaat acaaactgat aaggcgataa cagtaaactt aaatggtaca tatacaaaga
     2701 ctgtgactat caacatgcct aatggtgatg taaataacta tgcaaaagtg gatgatgtag
     2761 ttatagatga tgtaaaagat ggaacttttg ttaactacgg aaaaataact aacttaaagg
     2821 ttaatgataa aaatggagcc aagatagaaa ataattctaa aggtgagatt ggtagtttaa
     2881 cagtagcatc tggagcaagt caagtaaaag tgactaatgg tggtaagata actacagtta
     2941 caaataattc taaaggaact actatagata ataaaggtac aataagtagt gttaagggag
     3001 ataattctcc aacaataagt ggaaatagtc caagttctaa ctcttcagga ggaagttcat
     3061 caagtggagg ttcttcacat ggaggaggaa gttcttcatc aaaagtagat aaagtagttt
     3121 tgaaaaatac aataacagca gcaaataaat tgtataatga agcaatagag ggaacaaatg
     3181 taggtgaata taaagtaggg tcaaaagcaa tatataaaac agcaatagac aaagcacagg
     3241 caatattaga caaatctggt gtaacacaaa aagaagtaaa tgatgcagta acagcattaa
     3301 acacagcaac agacacattt aaagcaggaa aagtagtagc agtagataaa acagcattac
     3361 aagatgcagt aacagcagca acagcattac atgcta
//
LOCUS       DAELXX010000206         3370 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.208, whole genome shotgun sequence.
ACCESSION   DAELXX010000206 DAELXX010000000
VERSION     DAELXX010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.208"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            183..977
                     /locus_tag="KPH29_003619"
     CDS             183..977
                     /locus_tag="KPH29_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1469178.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEDI"
     gene            978..3038
                     /locus_tag="KPH29_003620"
     CDS             978..3038
                     /locus_tag="KPH29_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG1469179.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLPLLFACYIKADFKEVFSLTVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPAICEELFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILIMLGLRVLDRK
                     SLRN"
BASE COUNT         1311 a          334 c          535 g         1190 t
ORIGIN      
        1 tttataatgt aaaaaaatgt tacaaaagat attataaacg aataaaaaat aataatttgt
       61 cagataaact tgtaaatttt tatgaataat ccattctaaa acaaaaaact gtgtataaaa
      121 aaggtttata tgttatattt atataaatat aacatatgaa cgagaaagga gtggtttgat
      181 aaatgattga ggtagataaa ttgtgtaagt cttttacaag ggttgtaaaa gatgataaga
      241 ataaaagttc aataagaaag ctaaagaaag taaaaactaa aaaagaagaa ttttttgctg
      301 taaataatgt ttcatttaaa gttatggaag gtgaaattgt tggaatatta ggtccaaatg
      361 gtgcaggaaa gactacatta cttagaatgt taggtggcat attaacacca acatcaggaa
      421 gtataaatat ttctggatat gattattcaa tagatagaaa ttcagcaaaa aaagaaatag
      481 gttatctctc tggaaataca aaactatatg gaaggctttc tccgagagaa cttctaacaa
      541 cttttgcaag tctatatgag atgtcaaaag aagatataga agaatcaatt gaaaatattg
      601 taaaaataat ggatatgagt gaatttatag ataatagaat tgaaaatcta tcaactggtc
      661 agactcaaag aacatctata gcccgttgtt taatacattc tccaaaagta tatatttttg
      721 atgagccaac actaggtctg gatgttttaa gtagtgcatc tattatagat tttatgaaga
      781 atgaaaaact aagaggaaaa actgttcttt actcaactca ttatatggaa gaagcagaaa
      841 ctttatgtga caagattttt atgattcaca atgggaaaat aattgcatca ggaacaccac
      901 agagtttaaa agaggaatct ggagtaaata atttgagaga tgtattcata aaacttgcca
      961 gaaaggagga catttaaatg agaagtaaga ttgtaaaata catatttaaa aaagaaatgt
     1021 tagacatatt gagagataaa aagactttat ttatgatgat tgttgtgcca cttcttttat
     1081 atcctttact tatgttatta atgattcaag taatgaatat gtctgcaagt agtatgaata
     1141 gtaaggacat taatatagct tttaataata aacctaataa ggtgttagta tccatgattg
     1201 aagatgataa tttagaaaaa gatactaaaa atacttcaaa tgataaaggc aaaataagag
     1261 tgttagatgt agataactat aaaaagtcat tagaagaaaa taaaattgat gcttatataa
     1321 agatggaaga aaaaaaatca tctataaatt atcaaatata tataaattct tctcaagaaa
     1381 attcttctaa tgcactaaaa agaattgaaa ctgttttgga taaatataaa agatttacca
     1441 tagaaaaaac tttatctgaa aaaggattag atgtacaagc tactttagaa ccaataacat
     1501 atagcactgt agatgttgca aagacggaag aagtggcagg atacttttta ggtcagatac
     1561 ttccatttat attaataata ggagttttac taggttcaat atatccagca atagatgtta
     1621 tggctggaga aaaggaaaga ggtacattag aaacattatt tacactccca atatcaaact
     1681 tagaactggt tatgggaaaa tatatggcag tttctcttag tgcaatagta acagcaattt
     1741 taaacatttt atctatggga ctgactatgg catttatttt agttagtgga ggtattagta
     1801 gccaatttgg ctttggaaac tttaattatg gagacttagt cttaccaatt ataactactt
     1861 taatttgcat atgtcttttt tcaatggtag tttcagcaat aagtatgtgt gtatgttcat
     1921 tagcaaaaag ttttaaagat gctcaaaact acataacacc tcttatgtta attattatga
     1981 taccatcata tgtttctatg ataccaaata tagtattgga tagagtgaca gctgtaatac
     2041 ctgtagtaaa tatatcttta cttataaaaa gtgtattatc ttttaaaagc gatttaaatt
     2101 taatagcctt agttttagca tcaaatgtag cctttgttat attggcaatc atactactgt
     2161 ctaagatgtt caattctgag gaaatacttt ttggtaatag taagagtttt tctttcttag
     2221 agaaaagaag taatataaaa aaagggtcta taccaactgt aagtgatggt gttattttat
     2281 atgcagtagg attagttcta ttgatttatg ttggttcttt agttcaaatt aaatttggta
     2341 tggcaggtat tataatgaca caattcatga taatttcttt accattatta tttgcatgct
     2401 atattaaagc tgattttaaa gaggtattta gcttgacagt tcctaaatta aagcatgtgt
     2461 ttgggagcat tgttctttgg attggtggat acatacttat tatgattata acacagctaa
     2521 ttttattctt atttcctcaa aatatggaag ttgctgaaag tttaaataag gctcttttta
     2581 tcaaagatag tgttttactg aatttactag caatagcttt attaccagct atatgtgaag
     2641 aactgttttt taggggattt atattttctt catttagtaa atcaaaagat aaaaataagt
     2701 ctatcaaact cgctataata tgtagtgggg tactatttgg tattatgcat atggatttta
     2761 ttagaataat tccaacatca atattaggta ttatatttgc atacagtgtg tataaatctg
     2821 gttcaatatt tgtatcaatg ttattgcatt ttttaaataa tagtgtagca gttgtgttaa
     2881 atcattatac aactggaaat ataaccaact tatataaatt tgttgaagtt gatttttcga
     2941 acttaaatgt acttaaattt gccttaattc ttctaataag tcttatatta attatgttag
     3001 gattaagagt tttagataga aagtctttaa gaaattaagt ataaagttat tatatagatt
     3061 aaaatattaa taagcactaa agtatatgga tatatcttgt atataaaaat actttagtgc
     3121 tattttatat attttaatta tatgaattat ttatcgtttc atttatgtaa tgaaaatgtt
     3181 atataaatga aacaataaat aatttttatt ttagaaattt atatttaaat aatcaagttg
     3241 aaagtgagat actaaaaaaa ttaatttagg aattatttga tttattcatt tataaaaatt
     3301 aattactgat ttgtcgaaac cattgtaata agaaaaaaat gaaaaaataa agtgtgaata
     3361 tgttgcaaat
//
LOCUS       DAELXX010000207         3360 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.209, whole genome shotgun sequence.
ACCESSION   DAELXX010000207 DAELXX010000000
VERSION     DAELXX010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.209"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            567..3209
                     /gene="adhE"
                     /locus_tag="KPH29_003621"
                     /gene_synonym="adhC"
     CDS             567..3209
                     /gene="adhE"
                     /locus_tag="KPH29_003621"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBG1469180.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPDRVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGNTDQEKVDNLIKAIDELKARVGLPKTIKEAGVEESKFLETLDVMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
BASE COUNT         1302 a          425 c          634 g          999 t
ORIGIN      
        1 ttatcattaa aaaattaacg gtcttaatat aaaaattatt tatctatatg aaaagcataa
       61 ttattacata ttgacttaaa aataacttat agagattaga ataattttag tttgtatatt
      121 ttttaaaacc atacagttgt tataaaaaga aaaattgcac acaagtcaaa aataagtgtt
      181 attttataaa aatacgacaa aaattttatt tactacttat acaaataaca tgcaaataac
      241 atataaaatt taataagtaa aaaaaatgaa agatataaat tatcatttta gtattttatt
      301 tagttgaagt aaattattca aatgaaatat atctcattta gataaaaaat tttagtaaaa
      361 gtaaaaaaaa taatatattt gttaaaaaat aaattgacaa aatattcaga aaaaaacttc
      421 ttgacctaag agagtaaaat aaataaaatc taaggtatag agatgaaatc tataaaatta
      481 ttttaagtat aagttaaaaa tatagatggt gcatttaaga tttataaaaa ttagagatgc
      541 ataattattt tagggaggtt tttagtatgg agaaaaaaga aaaggtagta gaaaaatcaa
      601 atgttgaagt atgtagtcct gaaattgtca acagtgttga aacattaaga atgagattag
      661 aagaaattag attggcacaa aaagaatttg caacatttac gcaagaacag gtagataaga
      721 ttttcttagc tgcttcaact gcagctaatc aacaaagaat acctctagca aaaatggcag
      781 tggaagagac tggtatgggt attgttgaag ataaggttat aaaaaaccat tttgcctcag
      841 aatatatcta taatgcttat aaagatacta aaacttgtgg tgtcatagaa aaagatgaag
      901 catttggttt tacaagaatt gctgagccag taggtgtttt atcagcagtt ataccaacaa
      961 ctaatcctac atcaactgca atattcaaat ccctaatagc actaaaaact agaaatggta
     1021 taattttctc tcctcatcca agagctaaaa actgtactat agaagcagct agagttgtac
     1081 atgatgcggc agttaaggca ggagcaccaa aaggacttat aggttgggta gatgttcctt
     1141 cgattgaatt aactaatgtt gtcatggcag aggcagacct tatattagct actggtggac
     1201 ctggaatggt taaatctgca tactcttcag gtaaaccagc tgttggagtt gggcctggaa
     1261 atgtacctgc aataatagat gagagtgcag atataaaaat ggctgtaagt tcaatcttag
     1321 tatcaaaatc attcgataat ggtatgatat gtgcttccga gcaagctgta atagtaccag
     1381 aaaaaatata tgaagaagtt aaaaaagaat ttaaataccg tggagcacat ttcctaaata
     1441 aagaagaaac tgaaaaagta ggaaaagtag taataattga tggttcatta aatgccagaa
     1501 tagttggaca accagcacat gtaatagcca aaatggcgga tgtagaagtt ccaaaaacag
     1561 ctagaataat aattggtgaa gtagaatctg ttgaattaaa tgaacctttt gcacatgaaa
     1621 aactttctcc tgtacttgca atgtacaaat ctaaaagttt tgaagatgca gttgctaaag
     1681 ctgaaaaatt agttgcagat ggtggatatg gccatacatc ttcgttatat gcagactcta
     1741 taaatcatcc agatagagta gagaagtttg taaatgctat gaaaacttgt agagtattag
     1801 ttaatactcc atcgtcacaa ggtggtatag gtgacttata taactttaaa ttagcaccat
     1861 ctcttactct tggttgtggt tcttggggtg gaaactcagt atctgaaaac gtgggagtga
     1921 aacatttact taatatcaag acagtagctg aaaggagaga gaatatgctt tggtttagag
     1981 caccagaaaa ggtttatttc aaaaaaggtt gtttaggtgt agcagcaaga gaatttaaag
     2041 atgttatgga taaaaagaaa gcctttatag taacagattc tttcctttac aataatggat
     2101 acactaagaa gctaactgat ttgttggatg agatgggaat taagcatact acattctttg
     2161 atgttgctcc agacccaaca ttagcttgtg caagagaagg agctaaggct atggctgatt
     2221 tccaaccaga tttaataata gctgtaggtg gaggtagcgc aatggatgct ggtaagataa
     2281 tgtgggtaat gtatgagcat ccagaggtag acttccaaga tttggctatg agatttatgg
     2341 atataagaaa aagagtatat gtatttccta agatgggtga aaaagcgtat tttgctgcta
     2401 tcccaacttc agcaggaact ggttcagaag taactccatt tgcagttata actgaccaag
     2461 attcaggtgt aaagtaccca ttagcagatt atgaattaat gccaaacatg gctataattg
     2521 atgctgatat gatgatggag atgccaccaa gattaacagc agcatcaggt gttgatgctt
     2581 taacacatgc attagaagca tatgtatcta tgttaagaac tgaaccagct gacggtctag
     2641 cactacaagc aggtaaaata atatttgaat atctacctcg tgcatacaaa aatggaaaga
     2701 atgataaaga agcaagagaa aaaatggcaa tggcatctac tatggcagga atgtcttttg
     2761 caaatgcctt cttaggaata tgtcactctt tagcacataa attaggtgca ttccaccatg
     2821 ttcaacacgg tgttgcaaat gcattattaa taaatgaagt aattaagttc aattgtgcag
     2881 aagctccaaa taagatgggt gcattctcac aatacagata tcctgattgt atacaaagat
     2941 atgctgaatt tgcttctttt gcaggaatca agggaaatac agaccaagaa aaagtagata
     3001 accttataaa agcaatagat gaattaaagg caagagtagg acttcctaaa actataaaag
     3061 aggctggagt agaagaatct aaattcttag aaacattgga tgttatggtt gaacaagctt
     3121 ttgatgacca atgtacaggt gctaatccta gatacccatt gatgagtgaa ttaaaagaga
     3181 tatatctaaa agtttattat ggtaaataat attcattgta caacataggt tctattgggt
     3241 ttgtatataa attattagat ttgaatataa atagagtttt attagagaaa cgtagaagta
     3301 actataaata atagattata acttaaattc tgaatggaaa tgataaaaag tcagtaattt
//
LOCUS       DAELXX010000208         3321 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.210, whole genome shotgun sequence.
ACCESSION   DAELXX010000208 DAELXX010000000
VERSION     DAELXX010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.210"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            13..1797
                     /gene="pepF"
                     /locus_tag="KPH29_003622"
     CDS             13..1797
                     /gene="pepF"
                     /locus_tag="KPH29_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBG1469181.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYTKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEKYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLIHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            2049..2891
                     /locus_tag="KPH29_003623"
     CDS             2049..2891
                     /locus_tag="KPH29_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG1469182.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCSSNSGGSGDSGDSSKVSVSGS
                     TSIGPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSA
                     GLKETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREE
                     GSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSL
                     KINDVEATDANVMANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFI
                     KVNQ"
     regulatory      3124..3207
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
BASE COUNT         1353 a          350 c          600 g         1018 t
ORIGIN      
        1 gaggtgtatg atatgggaac tgatagaagt acaatagaag aaaagtacaa atggaagata
       61 gataagatgt actccagtaa agaggaaatt gaaaaagaca taagtaaagt aaaaaatctt
      121 atccaagaag taaaagaata taaagggaag ctttctgaga gtaaggagaa cttatataaa
      181 gtattaaata tctctgaaaa tgcatctagg attgttcaaa atttatatgt atatactcat
      241 atgaaacaac atgaagatac tagaattaat gataatcaag gaagagccac taagacagaa
      301 atgttatcta ctgatttagg tgtggctact tcttatatag ttccagaaat actggcgata
      361 gaagagaata agttaagtga atacttaaaa gatgagaagc tatcctttta tacaaaatat
      421 ataaaggata tattgagaga taaaccacat actttgagtg aacgagagga agaaatactt
      481 gcagctacat cagaattatc gacaattcca gaaaatgtat acgatatgtt agcatatgca
      541 gatatggaat ttcctgagat agaagatgag gagggtaata aggttaagct ttctcattca
      601 aactactctt tatttataaa aagtaagaat agtagagtta gaaaagacgc ttttgaaggt
      661 gaattttcaa catatgagaa atataaaaat acatttgcct caacgcttta tggtggaata
      721 aaatcagaga tattttacgc aaaaactaga aagtataatt cagcaataga agcgtcttta
      781 ttttcagatg atgtaagttt agatgtttac aataatctta tatcagctat agataaaagt
      841 attcctaatc taaataaata tgttgagctt aaaaagaaat ttttaggatt gaatgaaata
      901 cacatgtatg atttgtatgt tcctttgaca gataaatttg acatggatat accatatgat
      961 aaagcacagg atatcatatt agaggcactt aaacctttag gagaaaaata cttgaaggtc
     1021 ataaaaagtg cttttgatga agggtggatt gatgtatatg ataatgaagg taaaaaagga
     1081 ggagcttatt cttggggaag ttatgattca catccttata tattaatgag ctataaaaat
     1141 gaccttaatt ctctattcac tttaattcat gaattagggc actctgttca cagtcattat
     1201 tcaagaacaa gtcaaccatt tttatattcg gactataaga tattcgttgc agaagttgca
     1261 tctacattaa atgaattact tcttataaat tatttattag aaaattctaa gtctaaggat
     1321 gaaactatat acttattaaa ttattattta gaacaattta gaactacagt tcatagacaa
     1381 actatgtttg ctgaatttga aaaaattgta catcaaaggg tagagagtgg agaaccactt
     1441 actgctgatg aatttactaa tatatactac aaattaaatg aaaagtatta cggaaaatct
     1501 gcaatagtag ataaacaaat aggaatagaa tgggcgagaa tacctcattt ttattcaaac
     1561 ttctatgtat ataagtatgc cacagggttt tctgctgcaa gtgctttaag tcaacagata
     1621 ctttcagaag gaagtactgc agtagataga tatataaact tcttaaaaag tggagggtca
     1681 gaatacccac tagaacaatt aaaatctgct ggtgtagata tgactaaaaa agaatctata
     1741 gaggaagcct taaatgtatt tgctgaatta gttaataaac tagaaaaaga attataggat
     1801 gtgataggtt gatagcttca atgaagactt ctgataatat gagttgtatt gtgtttatat
     1861 ctgcctaaag taactttaaa aagttaatct aattaagaat aaatcatata aaattaagtt
     1921 tttacatgta tttaacaata tttacattta cttaaccctg ctctaatatt catttaacaa
     1981 agaatctgta ttattaaaat atagaaaaat gacaacaaaa aattaaaata taagcggggg
     2041 taagtaaaat gtttaaaaag aaaataggcg ttcttttagc tagtacaatg ttagttggag
     2101 ctttagcagt aggatgtagt tctaactcag gaggttcagg tgattcagga gattcaagca
     2161 aagtttctgt ttcaggttca acttcaatag gtcctcttat ggaaactatc ggagaaaagt
     2221 ttcaagaaaa aaatcaaggt gttagtgtag aagtacaaca gattggttct tccggaggta
     2281 taaaaaatgc catagaagga acatctgaaa taggaatgag ttcaagagat ttaaaagatg
     2341 aagaaaaaag tgctggatta aaggaaacta aaatagcaat agatggtata gcattgataa
     2401 ctaatactgc aaactcagta aaaaacctga caatggacca agttaaaggt atatatacag
     2461 gaaagattac taactggaaa gaagtaggag gaaatgatgc accaatagta atagtatcaa
     2521 gagaagaagg ttcaggaact agagatggat tccaagaaat tgtagggttt gattcagaag
     2581 aactaactaa agatgcacaa ataagtgatg gttcaggaaa tataaaaact acagttgaag
     2641 gaaatgaaaa tgctatagga tatatatcat ttggatatgt tgatgagaaa gtaaatagtt
     2701 taaaaataaa tgatgttgaa gcaactgatg caaatgtaat ggctaataaa tatccaatat
     2761 caagaccatt tatattagtt aataaagaag atggtttgtc agaaaaaggg aaagctttaa
     2821 tagattatat attaagtgat gaaggacaag cacttgtttc agaaaaagga tttattaaag
     2881 ttaatcaata acatgaatta taatataaag taaacatata ataccctgtg agataagctc
     2941 ataaaaatga tgctttaact tgcagggtat tgtctattat tgaatgtaga aatataaata
     3001 tctttagata aaataggtat tttagtatct tatatctaaa aatatggaaa tattgacaaa
     3061 ataatattac aatgttagaa taaaaaagaa atttatgaaa atatataaaa gatttttaat
     3121 aaaaatattt tagaaactga gaagtatatc ttattattgg gcatctggag atatatggag
     3181 ttagtggtgc aaccggctat gaatataatt tttatattgc atagtctttt tttgctttaa
     3241 aattaacaaa aatgttgcaa aatttgaaaa taagagtatt agtcgttaag atttttattg
     3301 ataggtgaaa ttttggcttt t
//
LOCUS       DAELXX010000209         3205 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.211, whole genome shotgun sequence.
ACCESSION   DAELXX010000209 DAELXX010000000
VERSION     DAELXX010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.211"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(145..666)
                     /locus_tag="KPH29_003624"
     CDS             complement(145..666)
                     /locus_tag="KPH29_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG1469183.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENIIIKTEVPETAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
     gene            794..2158
                     /locus_tag="KPH29_003625"
     CDS             794..2158
                     /locus_tag="KPH29_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG1469184.1"
                     /translation="MKESNKKIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYIARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLVVFGFIKGFQPIAGYNFGSKNYDKLHIAIKTSILWTTIFCVVLGL
                     ILVLFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKEGG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            2347..3024
                     /locus_tag="KPH29_003626"
     CDS             2347..3024
                     /locus_tag="KPH29_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG1469185.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSDYKKGNPVH
                     CGDSDCIGILIIKSGTIRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQINSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
BASE COUNT         1008 a          447 c          504 g         1246 t
ORIGIN      
        1 aaaagtttaa cattctccct caattaagtg agctttattt taaatatact tttcagttta
       61 tatcatttgt ttcatttttt ctaatatctc attattaaat atgctggttt ttgtttaatg
      121 attattaagt tagcttctct aattctaatt ccccatatta tcttttaaag aatttatcca
      181 ggtttccaat gcttgtgcta gcaccaactg ttgctccaac actgcttttg ctgtctctgg
      241 tacttctgtc tttataatta tattttcctc aatagttttc tttaactctt gaacattctc
      301 ttcaatatta ttgagacata atttctgttt ttctggtgta aggctattta aatttactat
      361 aacagcatta aaatctaaaa gaatatttat tggtttgcta gatgtatcaa acattaaatc
      421 taaaaaatgt gtctcaccta attcagttaa tgaataaact gctttttctg gcatttttcc
      481 attcttcaca gttttacttt caatatatcc cttttgttct aattgaatta cttttttata
      541 aattgatggt gtacttattt taacccaacg tgatatattc ctgtattcca ctgccttttg
      601 aatatcataa gcactcttgg gttgttgctt tactattccc aatacaatta aatctattgt
      661 agccataaaa atcatctcct ttagttttct tactattgac aaatactata aattatagta
      721 ctattttcca tacttactat aatttatagt attataattt agacttaatg tcaatagaag
      781 aggagtgtta agtatgaaag aaagtaacaa aaaaattgaa ttattagggt ctgccccaat
      841 ctcaaaagca ttgttatcta tgggattacc taccatgata ggtatgatga taactgcatt
      901 atacaatttg gttgatgcat actttgtggg tggacttgga acaagtcaaa tgggagctat
      961 atccgttgca tttccattag gtcagcttgt tgtgggatta ggattattgt ttggaaatgg
     1021 agctgcttca tatattgcca gacttttagg ccatggtgat aatgaaaaag caggaaaagt
     1081 ggtaagtaca tctatttaca gtagtattat agtgggtaca ttagttatta ttgtgattat
     1141 gatttttctc aatccaattt taaaattgtt aggagctaca aaaagtatta tgccttatgc
     1201 aatatcttat gctagcattt atgtcgtgtc atctattttt aatatattca atgtcactat
     1261 gactaatatt gtcacaagtg aaggagctgc taaaacaact atgtgtgcta tgttattagg
     1321 tgctttgctt aatatgattt tagacccgat attcatttat acttttggtc tgggggtaag
     1381 gggtgcttct attgcaacag ctatctcaca gattgcttca acacttgttt atgtaggata
     1441 tattttaagc aagaaaagta tatttagttt tagaattaac gaatgctgtt tttcaaaaga
     1501 aattttttca gaaatcctaa aaattggcat acctacatta atatttcaat tattgactag
     1561 tcttgccatt acattaacta atatggaggc tacaaagtat ggtgattctg tgatagctgg
     1621 aatgggtgct gtaacacgaa taacatcttt aggtagtctt gttgtatttg gttttataaa
     1681 aggtttccaa ccaattgctg gttataattt tggttcgaag aactacgata aattacatat
     1741 agcaataaaa acttccatat tatggactac aatattttgt gtagtgcttg gtcttatatt
     1801 ggtgcttttc ccaaaatcta taatttcaca atttacaagt ggtgacattc tattaataga
     1861 aaatggtagt aaagctctta gggcaaatgg gttttcattt atgctatttg gattctatac
     1921 agtgtattct acattattcc tagcactagg aaaagcaaag gaaggtggat ttttaggtat
     1981 gtgtagacaa ggcgtctgtt ttatccctat aattttgatt attcctgtat tctatgggtt
     2041 aaatggtatc ttatatgcac aaccaatagc tgatttgatg gctgctataa ttgctggttt
     2101 tatgtctata aggttacata aggaacttaa tcttttaaaa ataaacggta tgcattagtt
     2161 atacaaacct actatcatca aaacatatta ctttctcatt aatagcaaga atatatatta
     2221 tcatattttt tctacataaa tcatactatt actatctgac taaataatcc tcttaacaaa
     2281 ctacttgtca atttatctat tcacttgtat catgggatta taacaattta agaaaggaga
     2341 tattgtatga tttcacaaaa tgaaattgat atacttacac atagcttgcc tttttgggat
     2401 aaacttactg acttacaaaa agaattatta atttcttcag caaatacctc ggattacaaa
     2461 aaagggaatc cagtacattg tggtgatagt gactgtattg gtattttgat tataaagtct
     2521 ggaacaatac gtacatacat actttcagat gaaggtcgag aggttacact gttccgactt
     2581 gatgatggag atgtatgtat tttatcagct tcctgtattc taaaaacaat aacttttgat
     2641 gtctatgtag atgctgaaac ggactgcgat attatacaga ttaattcctc tgtttttgca
     2701 aaattatcta cagaaaatat tcatgcagag ttattttcat ataaattagc taccgaacgt
     2761 ttttctgatg ttatgtgggc tatgcaacaa attcttttta tgagttttga taagcgtttg
     2821 gcatcttttt taattgatga gatagctaaa aatggcagta gcactataaa tatgactcat
     2881 gagcaaattg ctaagtatat gggtagtgcc agagaagttg tatcgagaat gttgaaatat
     2941 tttgctagag aaggtattgt gtctttatct cgtggtggta taaaagtatt ggataaggat
     3001 aaacttcgtt ctttgacact gtaaaagtgt gtctcaaaat gaacttttta gttcatgagg
     3061 cactcttttt tgtgtgaaat caaatatatt ttcataattt caataataaa aaagatactt
     3121 atatctattt ttatatagta tcttatttga tatttacatt tattttttat gtatattttt
     3181 atattttaat aatttagttt aacta
//
LOCUS       DAELXX010000210         3131 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.212, whole genome shotgun sequence.
ACCESSION   DAELXX010000210 DAELXX010000000
VERSION     DAELXX010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.212"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            142..795
                     /locus_tag="KPH29_003627"
     CDS             142..795
                     /locus_tag="KPH29_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBG1469186.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVSASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKASEKIHGIIDGMKF"
     gene            871..1080
                     /locus_tag="KPH29_003628"
     CDS             871..1080
                     /locus_tag="KPH29_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1469187.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
     gene            complement(1220..1786)
                     /locus_tag="KPH29_003629"
     CDS             complement(1220..1786)
                     /locus_tag="KPH29_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBG1469188.1"
                     /translation="MMNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSP
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEKLVDRYFPDITKK"
     gene            1947..2600
                     /locus_tag="KPH29_003630"
     CDS             1947..2600
                     /locus_tag="KPH29_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBG1469189.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGLSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            complement(2758..2934)
                     /locus_tag="KPH29_003631"
     CDS             complement(2758..2934)
                     /locus_tag="KPH29_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1469190.1"
                     /translation="MYKYDDYVNLTNDLDLPVEIRNKCVRIVSVSENLEDIIIVHKFK
                     LYVVNEKYIKGIME"
BASE COUNT         1189 a          329 c          430 g         1183 t
ORIGIN      
        1 tataaatagt aaaaaagtag aagtaatgtg tataaaaaca taagttacat tctgtaataa
       61 tagttatttt ataattcaac aaaagaatag aatgggtata tttgtgaaat aataatataa
      121 aataatctta ggagtgatct tatgaagaaa aaattattat atataaatgt aaattcaaaa
      181 cctgaagact tatcttcaag taaaactgta gcaagaaaat ttataaataa atttatggaa
      241 aaaaacaaag attttgaggt tgaagagata gacctttata aagaacatat accgagactt
      301 gagtatcagt actttgaaaa aagaaatagc atagttagtg aagaaaatgc taaaaattta
      361 gacgataagg atagaaaaga gcttacaaag attagaaatc tttgtgacca atttgtgagt
      421 gcaagtgttt atgtaatagc agcacctatg tggagtttat cttttccagc accacttaaa
      481 gagtatatag attgtataat tcaagatgga aaaactatat cttttgaagg caatgataaa
      541 ccacaaggaa ttttaaatga tattgataga agtatggtat atatacaatc ttctggtgga
      601 cacataccat gggtcttgaa gccagttatg aataaagggt taaattatgt ggaaagtatt
      661 atgaaattta taggaattaa aaaatttgat gaattgttag tagatggaac tggaactagt
      721 gaagaagaaa gacaagctgc aatagaaaaa gcatctgaaa aaatacatgg tataatagat
      781 ggtatgaaat tttaattata atattttatt aaaaatataa aggaaaaacc tcttttaatg
      841 tagaaactta tattgaagga ggcgagaatc atgcgagaag aaaaaagtaa tgaaaagtat
      901 gattgttatt ggtgtaatca agaaaataat ttctgtgtag aaataaaaga taatatagta
      961 atgatagatg atggcactgg tacgttaaaa caagccgttt tcatagggta taaacaaatc
     1021 caaattaatt taaattgttc acattgtcaa aacttaaata gaataaaatt aaatttgtag
     1081 aattttaaaa tggaaattat tgaaggaatt aaaaatagaa agtgttaata tttagactga
     1141 aaattatatt ttatacaata aaagtttatt taaataacat tgtattttaa tatttatttt
     1201 cagtcttact ttattaaaat tattttttgg ttatgtctgg aaaatatctg tctactaatt
     1261 tttctgaaat attttttaat tggtcagcca ttgaagtcag acttaataag gctatcaata
     1321 aatcatcttt attttctgga tttatactaa tattttcaaa attagactct ataaagtccg
     1381 gtatattctt tctttgattt tcttttatta acatgaattt ttcgtaacat gaaactaaat
     1441 ctttttcact gaaattatcc ttttcataca caccatgtaa gattctttta atttcttgta
     1501 ttgtaagtgt atttttcata gaataaataa ttatcatctg taaaatttgt tctttagtat
     1561 acttttttcc cttaactggt ttgattaatc cctctttgct gtaattattt atcatggttt
     1621 tagtaagaat tttatcactt tcaaatcttt tattaggaga aagtttattg tcaattagag
     1681 aaataatttg atccatatat aagtcaatag agggaatatc gtttgaagtt atatctgcat
     1741 tatttagtgt ttctagtatt atagtattaa gttcattatt catcatatgt tacctccata
     1801 atatatataa tacactaaat tagaaagtaa aacaatatta agacttgtaa aaaaaacata
     1861 aagtggtata attaaatata taatatagta atgaaaacta tatttatgaa tctgattttt
     1921 aaaatacata attgtaggag gacgaaatgt atcaattttt tttaaagggt agagacccaa
     1981 taagcagttt aactcatttt ataggtgctt gtttatctct tttagcaact ataattttag
     2041 tttttcaatc tgttacatta caggaaactt ctctcttgat gatagtatct gtatcggtat
     2101 ttggactttc actaattgct ttatatagtg caagttcgta ttatcatttt ttaaagggga
     2161 caccagaaca agaattgttt tttagaaaag tagaccatgc aatgatttat gtattgattg
     2221 ctggctctta tacaccaata tgtctgaatt ttatggaaaa aaaagaaggc ataatatttg
     2281 ttacagcaat atggatagtt gcatttattg gcataataat aaaaatattt tggatggatg
     2341 cacctagatg gttatctacc agtatatatt tacttatggg gtgggcaatt gtttttgata
     2401 taaatgcatt caattcaatt cctaaggatt gccttagact attaattatg gaaggtgtat
     2461 cttactctat aggtgcaatt atttatataa ttaaaaagcc taatataagt cctgagtttg
     2521 gatttcatga aatatttcat atttttatta tgataggttc tttatttcac tttttagcag
     2581 ttttattgta tgttttatag ttttaaaaga tttgattaat tgatactaaa attctacaat
     2641 gaataaaatg gaactgcctt atatagatta tatcttcgac aataagacag ttccttatta
     2701 taaatattaa attctactta taataaaatt ttgataaaaa tagtccattc aaaatgccta
     2761 ttccataatt cctttaatgt acttttcatt tacgacatat agtttgaatt tatgtactat
     2821 aattatatcc tctaaattct ctgaaacact aacaattctg acacatttgt ttctaatttc
     2881 tacaggtaaa tctaaatcat ttgttaaatt cacatagtca tcatatttat acataaaatc
     2941 atccctttac attaagatat acttattata ctatatttta ataagttttt ttaaattatt
     3001 acaatttttc ccgaaatttc attttttata cataaaattt ccaaattagg taaagattaa
     3061 attttttatt gtataaacaa aatttacata taaaatattt tattcaaatt tttaaataac
     3121 tattgccaat t
//
LOCUS       DAELXX010000211         3010 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.213, whole genome shotgun sequence.
ACCESSION   DAELXX010000211 DAELXX010000000
VERSION     DAELXX010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.213"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            340..1617
                     /gene="hflX"
                     /locus_tag="KPH29_003632"
     CDS             340..1617
                     /gene="hflX"
                     /locus_tag="KPH29_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBG1469191.1"
                     /translation="MDMTKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSIT
                     QNAKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRT
                     ALILDIFAQRAKTREAKLQVEVASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKL
                     ELDRRRIEEKITSLNRELYDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLV
                     DIFKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRS
                     TLEEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVEVL
                     DRILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDCVRCKFLIPYDKGHVVSYFNEN
                     TSIINTEYREDGAILDVECSNIEYNKYKKYALE"
     gene            1850..2347
                     /locus_tag="KPH29_003633"
     CDS             1850..2347
                     /locus_tag="KPH29_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG1469192.1"
                     /translation="MDAESLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIQIEQNEKDSRAI
                     CLVTKDRVQEYSKKVRGMQEKVLQLLFEDFTEKEVEQLFFGIMKLSIGIENVKNYVEN
                     NDVGE"
     gene            2397..2921
                     /locus_tag="KPH29_003634"
     CDS             2397..2921
                     /locus_tag="KPH29_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBG1469193.1"
                     /translation="MKSVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKDLQKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKVPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KENLTPILNYIRKL"
BASE COUNT         1250 a          268 c          526 g          966 t
ORIGIN      
        1 atataaaatg ataattacat ataagttaat taaactaatg tatttttaat atatatttag
       61 aattttaatc cttcttatga tataagtagc ttgttttgta ttacttagat ttgtaaggag
      121 tttttttatt gaaaaaactg cataatatat ttgggaggag gtgtaagaaa gtgatttttt
      181 atataagatt gagaaaataa tgcacgatta tggttattat aatagtaaat aagaagtgtc
      241 atttaaggat aagataaaaa gcaaaattct tgatgaattt aattatagag ccataatctc
      301 gtgctataga ttaaataaaa aatagcagat aaggagaata tggatatgac gaaaaaagga
      361 ataacagtag gattaaacat aaataataaa tctgaggact ttaatgaact aatgattgaa
      421 cttgaaaact tatgttcagc atgtgatatt gatgttgtag ggagtataac tcaaaatgca
      481 aagcaggtta atagagcatt ttacattggg acaggtaaag ttgaggaaat attaaactta
      541 ataaagaaag aaaacataga gatagttata ttttacaatg aactttctac atcacaacta
      601 aaaaatcttg aagaaaagct taattgtgaa attatagata gaacggcatt gatacttgat
      661 atatttgcac aaagggcaaa aactagagaa gctaaacttc aagtagaagt agcaagttta
      721 aaatatatgt taccaagact tataggttca aatgaaaatt taggacgtca aagtggtgga
      781 gttggtacta aaaatagagg ttctggtgag aaaaaattag aacttgaccg tagaagaata
      841 gaagaaaaga taacatcttt aaatagggag ttgtatgatt taaaattcca aagagaaact
      901 caaagaagta tgagaagaaa atcaaatctt ccaaatgtag ctctagtcgg gtatacaaat
      961 gctggtaaat cttcaataat gaataaacta gtagatatat ttaaaaactc tgaagaaaag
     1021 aaagtgtttg agaaaaacat gttatttgct acacttgaaa cttctgtcag aaatatagtt
     1081 cttgcaaata acaaagaatt tttattatct gatactgttg gttttgtaag taatctacca
     1141 catgacttag taaaagcatt taggtcaaca ttagaagaag catgtgaggc agacgtactg
     1201 ttacatgtta ttgatatttc aaatccaagc tataaaagtc atattaaagt gacagaagat
     1261 actttaaaac aaataggtgc agatggtata cctatgatac atgtgtataa taaaattgat
     1321 ttaatagatg tagaagtatt ggatagaatt ttagatagta tcgataaaga aggtatattt
     1381 gtttcagtta aaaaagatat aaatatagat aaaatgataa aatgtatttg tgatagcata
     1441 tttaaagatt gtgtaagatg taagttctta attccatatg ataaaggtca tgtagtttct
     1501 tattttaacg aaaatacgag cattataaat acagaataca gagaagatgg agctatttta
     1561 gatgtagaat gtagcaatat agaatataat aagtataaaa agtatgcttt agagtaaatt
     1621 ttgaaaaact tattatagcc taaaatatat ttaatttata atttattata aattgattgt
     1681 aaatatgatt ttatgaaacg gtatagccat aatgctatgc cgttttgcca tttataataa
     1741 aaataaattt attgacacaa tagtaactaa ttactataat ataaagtaac tagttactat
     1801 ttaaaattat caaaaaatta aaatctaaga taaagagaag tgtataataa tggatgcaga
     1861 aagtttaatg aaaaaatata tggtagatga tgatagtaga aaacaagcta tatttgcaag
     1921 tatatttgta atgcaaaata aacttcaaac tacatgtgat aaattagacc cagaattaac
     1981 aatgaaacaa tggttacttt tagctattgc ggactctgta gatgaaactc ttacattaac
     2041 caaacttgga gaattaatgg ggtgctcaag acaaaatgta aaaaaactag ctattgcttt
     2101 agaaaagaaa ggttttatac aaattgagca aaatgaaaaa gattcaagag ctatctgtct
     2161 tgtgacaaaa gatagagtgc aagagtattc taaaaaagta agaggtatgc aggaaaaagt
     2221 attacagctt ttatttgaag attttacaga gaaagaagtg gaacagttat tttttggaat
     2281 tatgaaatta agtataggaa ttgaaaatgt gaaaaattat gtagaaaata atgacgttgg
     2341 agaataagca agtatcaaat caactttgta tgacatcaga gaggggggta atttatatga
     2401 aaagtgtagt agtgatttat aagagtaagt atggttcaac aaaaaaatat gctaaatggc
     2461 tttcagaatt attgtcttgt gatatttttg aaactaagaa tattaatata gaaaaggtta
     2521 tgaagtatga tacgattatt ttaggaggag gtctttatgc ttgtaatata gcgggaattt
     2581 ctttcttaaa aaaaaattat aaagatttgc agaaaaaaaa tttagtagta tttggcgtag
     2641 gagctgctcc atatgatgat gaagtaataa agaaattaag agaagtaaac tttaaggatg
     2701 aattaaacaa agttccttgc ttttattgtc gtggggcatg ggatgagaca aaaatgtctt
     2761 ttatagatta tactctttgt agttttttta aaaaatctat agctaaaaaa gaacgtcaag
     2821 gtcttaaacc agcagaagca cctttattgt ctggagaagc ctttgattgg tgtaataaag
     2881 aaaatttaac tccaatactt aattatataa gaaaacttta agaggtatat atttataatt
     2941 atttattatc taaagttaga tttcatagaa acttactaga cattatttat aaaataatat
     3001 tattaaagat
//
LOCUS       DAELXX010000212         2945 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.214, whole genome shotgun sequence.
ACCESSION   DAELXX010000212 DAELXX010000000
VERSION     DAELXX010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.214"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            12..881
                     /locus_tag="KPH29_003635"
                     /pseudo
     CDS             12..881
                     /locus_tag="KPH29_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
     gene            1787..2494
                     /locus_tag="KPH29_003636"
     CDS             1787..2494
                     /locus_tag="KPH29_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG1469194.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
BASE COUNT         1193 a          256 c          469 g         1027 t
ORIGIN      
        1 attataaagg agtggttatt attaaggcaa ttgaacttta taaaaaatta gatttagaat
       61 ttaatattaa aaatattaat gatgattgga gttttatgaa ttttgaaaat gattttatca
      121 ctccagaatt tagaaaaaag tacatatgat tagtattaga taatgctcaa aatattaata
      181 aagtatacac aactactttt cctgataaag aaataattaa gcaagttata gataaagatg
      241 aaaaagatat tttaattttt tcacatcacg caatgggata catagcatca gatgaaggtt
      301 ttccatttca cgacatacca ctatcttata tggaagagat gaaaaatcgt agaatttctt
      361 tttatgtatt acattctcca ctagacaact atagtgacta ttcaacttct gttagttttg
      421 caaaagctat gggtttagaa atagtcaaac cattttgtaa atatgacgat aaaattgagg
      481 taggagttat atgtaaaaca tcactaaaat caatagaaga gattaaggaa ttaattaaaa
      541 aatctgttgg tcatgatgtg aaattgtatg attatggaga ttctgtatta aaagatggtt
      601 tagttgctat tgcagcaggg ggaggaagtt atccatttgt agcaagagaa gttgctgaac
      661 ttggtataaa tctatatatt actgggttta ctaagccttt aaaacatttt gaaccagtat
      721 tagagttcca tcaaatagca aaagataatt taattaatgt tataggtgct actcattatt
      781 ctacagaaaa atttgcgtgt atgggcatgg ttgattattt taaaaatcta ggtttagaat
      841 cagagttttt gcaaggaaaa tattacttag aggatttata ggacatatta aacaataaaa
      901 ataaacagtt agtaaaaatt tattttacta actgtttatt tttttaccta tttatccagt
      961 gttttgaaaa aatctatctt tattttttaa actaagaaaa tagactttta attgttgatt
     1021 agaagattta gttttgatgt cttttaaatt atttttcgat attttttagt ttattttttg
     1081 atgtatttta gattattatt taggattgat tagaatttat aaatatttat tttagattat
     1141 tgtaaatatt tatttaaaaa aaatagaatt attatattta tgataataaa tggtataata
     1201 aaaggtaaag tgtcaaatat gcactaaatt aaataagtta attagtatat aggttaaagt
     1261 ggaagtaatt aaaacttatt aagtattaga aaatgacttc ttattagagt aagtatctaa
     1321 aatgatataa aaattatgat tactcattaa agtgactatt atagagtaga taaaattgcc
     1381 cataaaaata gctataatag agtggataag attactcatt aaagcaatta ctatagagta
     1441 gataaaatta ctcactaaaa taactataat agagtggata agattattca ttaaagcgat
     1501 tactgtggag tagataaaat tactcactaa aataactata atagagtaga taagatactc
     1561 attaaggtga ttattgtaga gtagataaaa ttacccatca aagttaatta tcacatatta
     1621 gataagatta ttcattaaag ttactatcac agagtggata aaattacaca ttaaaattgt
     1681 ctgttaaaga atgaataaaa aagagattgc tcaatataag aagcatataa aaaatcaaac
     1741 atataaaatt taataaataa taactgatag ggaaaggaaa gatagaatga tatttacaaa
     1801 aagcaataaa tacgataagg actttctcat gaaaaatatg atgggaccta attgtataaa
     1861 aatattggaa gaattaacaa gtaagataaa actagaaaag ggaatgcgta ttttagactt
     1921 aggttgtggt aagggtatat cttcgatatt tttagcaaag gaatttgatg ctactgtatt
     1981 tgcaacagat ttatggatag aaccaactga aaactataaa agatttaaag agtttaaatt
     2041 ggatgataaa atatttccaa tacaggcaga ggcacatgaa ctaccttatg cagaaggttt
     2101 ctttgatgcg gtgataagta tcgattctta tcactatttt ggaaacaaag aaggattttt
     2161 agacaatcat atatcacctc ttgtaaaaga ggggggaata cttgctatgg caatgcctgg
     2221 attaaaagaa gattttgtgg attgtatacc agatgaactc attccatttt ggcaagataa
     2281 tatgaacttc cattcaatta catggtggaa taaactttgg tctgaatcgg agtctgtgat
     2341 tgtagagaaa tgtgaagctt taaattgtca tgatgaagct tggaaggatt ggataaattg
     2401 tgataattca tatgctataa atgacaaaaa aatgatggaa gtagagaatg gaaagtattt
     2461 taatacaatt tctttgatag cgagaactaa ataattgtga tgaatttata aaagtataag
     2521 ataagtagaa atataagttg aatgagagta taagaagcat agaaatataa gttgaattaa
     2581 agtagaaggt aaataaaaat gtaagttaaa taaagagttt atacaaaaat gattttttag
     2641 attatttata cccaatcgag ttgcttaggt cagttgggta tttttatttt tttaaagtta
     2701 aattgcaaat actattgtgt gatatatatt ttaatactaa atgtttgttt tataagaaat
     2761 aaactaatag aaaaaatagt tttaacaatg aaagcaaaaa aggtattgat gaagattaaa
     2821 aattttatag taccatttta tatatttttt tatatatttt attatacttt aaatcaaatt
     2881 tcaaaatgat taagtatata tacccaaaag attaaagaac tatatttatt atactattaa
     2941 aaatt
//
LOCUS       DAELXX010000213         2928 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.215, whole genome shotgun sequence.
ACCESSION   DAELXX010000213 DAELXX010000000
VERSION     DAELXX010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.215"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(148..414)
                     /locus_tag="KPH29_003637"
     CDS             complement(148..414)
                     /locus_tag="KPH29_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG1469195.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTDIEKPFWGEQCPVKSCCED
                     KNLIHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
     gene            complement(457..1266)
                     /locus_tag="KPH29_003638"
     CDS             complement(457..1266)
                     /locus_tag="KPH29_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1469196.1"
                     /translation="MNKIINNILNTCKQYTYILPHKLLRQYIAHYTISIPDTSIKEKL
                     TLIPDASGCMIFKFDKKGIESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTG
                     LSQRESTDLKISLEDFNTLFSLEVNSIFERTSTIKELVEQLDMLFLSYLLKSNIVDMT
                     IPILENARKQNSIMSVKNISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLVLQEV
                     QNNKRPFATLAQDIGYYDQSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
     gene            1579..1860
                     /locus_tag="KPH29_003639"
     CDS             1579..1860
                     /locus_tag="KPH29_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1469197.1"
                     /translation="MASKQTPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
     gene            2124..2378
                     /locus_tag="KPH29_003640"
     CDS             2124..2378
                     /locus_tag="KPH29_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1469198.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
     gene            2423..2746
                     /locus_tag="KPH29_003641"
     CDS             2423..2746
                     /locus_tag="KPH29_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1469199.1"
                     /translation="MATEIFTVKITPLEAQKMIKENIDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTEKTVIKATSTGSAIGVLKIDWGAGGNLLKRVKNIL
                     AEYII"
BASE COUNT         1148 a          310 c          393 g         1077 t
ORIGIN      
        1 ttagtaatca gattttatca ataaattact attctattaa tttaattaaa taacctttaa
       61 atttaattat aaaaactgtt gcagaaattt ctacaacagt ttaaaaaatt taccttacaa
      121 aaacttattt tataacaaga gagtatatta taaggctagt acagcccatt ttttgcactg
      181 agaaattctt ttgccatcat caccttgttc tttgtcatat gcaaattgat taagcatttc
      241 acatggaaat gtatcacata gaccacaatg tataaggttt ttatcttcac aacaagattt
      301 tacaggacat tgttcacccc aaaatggttt ctcaatatct gtacaacctt tacaattgac
      361 tttttccttg tatccacatt cataacataa aattccacat cttgattcta tcattttaag
      421 tcactcctta aatataaatt ttattatgat tgtatatcaa aatttatact tttcattgta
      481 aaaatcggac aaatttttta tataggttgt tggattaaca ccacaaatag atttaaaatc
      541 atttataaaa tgagattggt cgtaataacc aatatcttgt gctaaagttg caaatggtct
      601 tttattgttt tgtacttctt gtaatacaag gtttatacga agtaatctaa ggtatgattt
      661 aacactcatc ccaagagaat tattaaaaat tctatttaaa tgtcgttcac tataacaact
      721 aatttgtgaa atgtttttaa cagacataat agagttctgt tttctagcat tttcaagaat
      781 tggtatagtc atatctacta tatttgattt caataagtat gataagaata acatatctaa
      841 ttgttctact aattccttta tagtacttgt tctttcaaaa atggaattaa cctctaagct
      901 aaataatgtg ttaaaatctt caagtgaaat ttttaaatct gtagattctc tttgagaaag
      961 acctgtcaaa taatatactc cacctgggcg aaactcaacg aatactctaa aaagtacatt
     1021 ttctatatca ttttttacaa tagtagtttt tgttgtggct ccccagaatg cgctttctat
     1081 accttttttg tcaaatttaa atatcataca tccactagca tcaggtatta atgtaagctt
     1141 ttctttaata cttgtatctg gaatagatat tgtatagtgt gctatatact gtcttaacaa
     1201 tttatgtggc aaaatatatg tatactgttt gcaggtattt aaaatgttat tgattatttt
     1261 attcataagt atatcctcct gatatataaa aaataagtat actaatttat tttttatata
     1321 ataaaccagt tttgttttaa ttgaaatatt ctaataagat attttgttaa atttagatac
     1381 aataattgta aagtaaaatc tcaaaatata caattattat aaatcataga tatttattaa
     1441 ataaatatat taaagaagaa ggaatataaa acaagattag aaaataatag tgttactttc
     1501 ataataaata catataaaga tatttttaag aataaaattt attattaaat aaaaaataaa
     1561 ggagagtgat ttaatcgtat ggcatcaaaa caaacaccaa gaaacaattt agaaaagttt
     1621 ttaagaataa gagtcttatc acatacaggc gataaaatac atataaagtt acctgtaaat
     1681 tttgcaaaaa gaatgattga gaataatgct ttggatttat ttaatggaaa agatgatgta
     1741 gtagatggta aaaaggttac tgaaatatta gtcaaagcat ttgactatga tttaactgga
     1801 gaagtagttc acttggagag aaaaaatgga gatattatta aagtttcaat tgtacaatag
     1861 aattatttta tatgaatttt atttttgaat agttataaat aattagaaat ttgtaaaaaa
     1921 gtataatata ttattagtaa tggtgcagaa tacacattta taatttatag atgtgtattt
     1981 tttgtttaaa aaatttaaat atataaaact ataaaggtta caattttgga aatagatgta
     2041 aatatatgtt ctattgtata ataagagtaa tataaaatct agtaaaaata atcaaataaa
     2101 tttaacttat gagagggata actatggaaa ataaggattt taaattaaca atatgtatat
     2161 ttgcttttgt aattggagta gtagtattat tctgctgtat aggtttgggt gaatttagtg
     2221 cagagcatat tattcaacaa aatggtggaa tcatggatac tgaccaatat aatatttatc
     2281 tagaccaatc tatttctcaa tatagaaatt ttggttctat attagctctg ttaggaggaa
     2341 tagggatatt gtttgataaa tatatcagaa aatactagag attatgtttt gtctaaaata
     2401 aattcttatg aggagataaa aaatggcaac agaaatattt actgtaaaaa ttacaccctt
     2461 agaagcacaa aaaatgataa aagaaaatat tgatgcagat ttagttttta gtgattctta
     2521 taatttagga gatgataaat ttatactaat tacaactttt gagaagtatt acgcaagaaa
     2581 taatagtgat gcaggattaa tcgtagttta tgaaaatacc acagaaaaaa cagttattaa
     2641 agctacttca acaggcagtg caattggagt tttaaaaatt gattggggtg caggtggaaa
     2701 tttacttaaa agagtaaaaa acatacttgc tgaatatatt atttaattat tttatctaac
     2761 tgataatatg tcaagaaaat ctcgtaaaag aggttaagaa tatttttata atatatagat
     2821 aaataaggat ttgaaggtaa agtttttaat gtataaagaa aaataatata ataaaacgaa
     2881 ttggctttat aattctttat aaggcaaaga gtacatttct ttatataa
//
LOCUS       DAELXX010000214         2887 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.216, whole genome shotgun sequence.
ACCESSION   DAELXX010000214 DAELXX010000000
VERSION     DAELXX010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.216"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            474..1517
                     /locus_tag="KPH29_003642"
     CDS             474..1517
                     /locus_tag="KPH29_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1469200.1"
                     /translation="MLKKWFGIVKKRQKSESVKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNEILTKVEVVNDDNEIEEKIIEEDWLISENTIKLDDKEAIINDKNIELCKKEVQ
                     VESEKIGLNKFEGLDQNENYNLEKNVIEEKKVSECLTEEDLEYIKEIKIKRGKSIKAI
                     NLYTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKG
                     EWSYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYD
                     EYFKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTK
                     VGRNFIRYSLRSI"
     gene            1628..2770
                     /locus_tag="KPH29_003643"
     CDS             1628..2770
                     /locus_tag="KPH29_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG1469201.1"
                     /translation="MSEEKNPKENTLDEPKNSDNVNSKKDDVSDEKNTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
BASE COUNT         1293 a          230 c          500 g          864 t
ORIGIN      
        1 gtttcaaggt ctatatgatt aattaaatcc atttttgtta tttataatta ttattgaatg
       61 tatttttaca tataagatat aaggtgatag atagaaattg ttggattatg taaataatta
      121 atatataata ccattatggg caataaatac aaatcaaatg ataatatagt ctatttatgt
      181 aaatgttata ttgtatccta agtataatag taagtttttt gatagtggtg tagaaagtaa
      241 aaatctacta gcctaaattg atgtatggat tagtttagga acagtaattg catatcttaa
      301 gtttgatgtc atgttgggct actagaaata ggtcatcttt ttcacttgaa tatcttttct
      361 ttaaacatga ggagtatcat atcaaaaaca tatgaaaatt aagtgattta taagaacagt
      421 aatattttat atgatagtat agaatagtaa taaagtatta ggaggaacat tgagtgttaa
      481 aaaagtggtt tggtattgtg aaaaaaagac aaaaaagtga gtcagtaaaa gaagaaggtg
      541 aagtaatatt aaaaaacgaa aaaatattat ctgaagaaaa gttgatagat gaagaaggag
      601 ttgtagttaa tattgataat gaaatattaa ctaaagtaga agtagtaaat gatgataatg
      661 aaatagaaga aaaaataata gaagaagatt ggttaattag tgaaaatacc ataaaattag
      721 atgataaaga agcaattatt aatgataaaa acatagaatt atgtaaaaaa gaagttcaag
      781 ttgaaagtga aaaaataggt ttaaataagt ttgaaggact tgaccaaaat gaaaattata
      841 atctagaaaa aaatgttatt gaagaaaaaa aagtaagtga atgtttgaca gaagaagatt
      901 tagagtacat aaaagaaatt aaaataaaaa gaggaaaaag tataaaagct ataaacttgt
      961 atactaaaga agagtgggtt tttgacactc atatacagtg tagtaaaaaa ctcaaagttc
     1021 cattaggata cataagagaa aatttaaaat atggatatat ggattacttt ggagatgcaa
     1081 taaattattt aagtgaagta ttaaatatag atgaatactg caaaggtgaa tggagctatc
     1141 tcgataatag caaatctcca tctgagatat ttaatattct aaacaataaa atatttagta
     1201 taaggctttc taatgaaaaa agaaatgaaa ttttgacaaa tgataaaatt gaagcattaa
     1261 aaatgaatta tagatttgaa tgtattgatg aagaatatga tgaatatttt aaaaaatata
     1321 agtctataat caaaaggggt ggaaagaaaa aagttgaatt agtaaataaa aaaggtgaca
     1381 ttttagaaat atttaagtct ttagaagaat gtgccattta tttgcaaaag gaaaagaatg
     1441 aagttataca aatgttaaaa tatggagata caaaagtagg aagaaacttt ataaggtata
     1501 gtttgagaag tatttaattt cattttaaat tcataagagt ttcatttctg attcacacat
     1561 ttaatattat tttaatgtaa aataataaag gtgaagtatt gcacaattaa aattaaggag
     1621 ggtattaatg tctgaagaaa aaaatccaaa agaaaataca ttagatgaac ctaaaaattc
     1681 agataatgta aattctaaaa aagatgatgt gtcagatgaa aaaaatactg ataacaaagt
     1741 taaaaaaagg gaaataaagc taaacaacaa aacacttaaa gctattgtag caattgttgc
     1801 agtaggagct atatgtttag gtggaggcta tgtttttggt aaagaagctg gaagaaaatt
     1861 gcctgctact tcaaaacact ataatagcag tgaggtagta gctactattg gggataaaaa
     1921 aataacaggt gaaatgttaa gaaagagaat ggagccatta ttttatttaa atgcaaaaac
     1981 tactatgact gatgacgaaa tacgttcata tgaatcaaat atgattgaaa ctatgattac
     2041 atctgatata ttatcctctg aagcagcaaa agagaaaata agtataacta atgaccaact
     2101 agatgctcag tataataatt taatgagtag tatagaatca gcaatgggaa tgactaaaga
     2161 agagttttta aaacagtttg atttgactga aaaatatata aaagatgata tgaaaaaaga
     2221 gctagtagca tcaaaatatt taaatgaaaa atcaaaagtt actgataaag aagcagagaa
     2281 ctattacaat aaaaacaaga gcaattattt acaagttaga gcttcacata ttttaataaa
     2341 aactgttgat gataaaggta agcaagttag tagtagcaaa aaagctgaac ttaagaaaga
     2401 agctgaagaa atacttaaaa aagcccaagc tggggaagat tttgctactc tagctaaaaa
     2461 atattctgaa gatagtagtg cagaatcagg tggagattta ggtttctttg gtaaaggtca
     2521 gatggtggaa tcttttgaaa aagcagcctt tgcattgaag aagggagagg tatctaacaa
     2581 acttgtagaa agtgattatg gatatcatat aattaagaaa actgatgaaa aatatcaacc
     2641 atttgaagag gtaaaatcag atttagtaag cagtttaaca tcagaaaaac aaaacctact
     2701 tatacaaaat ttaaaagaaa aatataatgt taaaattaca gataaatatc aaatgaaagc
     2761 taaagaatag ataaagtcaa tttattaaaa aaaagaaaaa ttaactgtat ggataaaagc
     2821 tatttttact ctcaatatag agagtctaaa gtagctttta ttattttgta ataaaatcta
     2881 tttatat
//
LOCUS       DAELXX010000215         2840 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.218, whole genome shotgun sequence.
ACCESSION   DAELXX010000215 DAELXX010000000
VERSION     DAELXX010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.218"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            331..1950
                     /locus_tag="KPH29_003644"
     CDS             331..1950
                     /locus_tag="KPH29_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBG1469202.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDERFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGNCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
     gene            2324..2518
                     /locus_tag="KPH29_003645"
     CDS             2324..2518
                     /locus_tag="KPH29_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG1469203.1"
                     /translation="MINYMVEIIYSEKISEVENMLKIENIQYEFTSRDGFIFFGFYEM
                     TDAYRFETLCSDEKIRFKRI"
BASE COUNT         1064 a          331 c          386 g         1059 t
ORIGIN      
        1 atataaaaat atttataact aaaaaatatc attctaaaag gatgctattt ttttacatat
       61 tttttacaat tatagttaat tctttgttaa attgattgta cataaaatac taaaaatcta
      121 agttcaagta tttaaattgc tatatatcat aaattttcta aaattaacaa caacttaaaa
      181 atttaacatt ctcttaacgt atacttaaca aaacattaat atttctattg acaaaatttg
      241 ttattctata ttatggaaac aataaatttt agttaataaa gtttataatt cagataaaaa
      301 aactattaat ttcttaaggg aggaaatatc gtgaatatag taattagcct tatcggtgga
      361 cttggacttt ttctttacgg aatgagttta atgggagaag gtttacaaaa atcagcagga
      421 gacaaattaa aaaaaattat tgaactctta acaagtaatg ttgttatggg tgtactggta
      481 ggtacagtcg ttacaggtat tatacaaagt tcaagtgcta ctactgttat ggttgtagga
      541 tttgtaaatg caggtataat gaatctaagt caagctattg gtgtaataat gggggcaaat
      601 ataggtacta cagttactgc tcaattagtt tcatttaatc ttgaaggaat tgcaccaatt
      661 gctttaggta taggtatttt attctattta tttacatcaa atcaaaaaac aaagcattta
      721 tctgagatac ttataggttt tggtatatta tttacaggaa tggagtttat gaaagatgca
      781 gtagctccac ttgctgaata taaagcattc actgatgcac ttttatattt tagtaaaaat
      841 cctgtattag gtatattggc aggatttgcc ataactggta ttgttcaaag ttcaagtgca
      901 tctatgggta tgttaatagc actagcttct caaggaatac ttcctctatc atctgcttta
      961 cctattcttt atggagataa tataggtact tgtgttactt cacttttatc aagtgttggt
     1021 gcaagtagaa atgctcgacg tgcagctgta atgcatttaa gttttaatgt cattggtaca
     1081 ataatattta tgcttgtttt aaataaacct atatcagcca tagttactca ttttgaccca
     1141 actgatacag ctagacaaat agcaaatgca catactttat ttaatcttac aaatgttata
     1201 atattattac cattttctaa atacatagtt aaattagcaa atagacttat acctattaaa
     1261 gaaactgaat cagaaatagt aaataatact aagtacttag atgaaaggat gtttagcact
     1321 ccatctatag cacttggtaa tactgtacaa gaagttgtaa gaatgggtca taaagctaca
     1381 aactctttgg aacattcaat tgcaggattt ttaaataaga gcaatgaaga tattaataaa
     1441 gcttttgagt cagaaaaagt tgtcaataag ttacagaaag atatattgaa ctatttgtta
     1501 aaactttcta aagaacctct tagagacgat gaacgcttta gaacagattt gctttttaat
     1561 actgtaaatg acattgaaag agtctctgac catgcagaaa atatagctga attggccatg
     1621 agtgttaagg aaatgaacat tagtttttct gatagtgcga taagagaaat atacgaaata
     1681 tacaataaga caattactaa ttttaaggat gccttaatag ttttagatgt taaagatttt
     1741 gagttagcaa ataaagtttt agaagttgaa aatgaagtaa attatttaga aaaaactttt
     1801 agaaattcac atatgattag actaaataat ggaaattgta caattgatgc tggtgtactt
     1861 tatcttgact tacttactaa tctagaaaga atttctgacc actcaacaaa tatagtaaaa
     1921 caagtattaa aacttaaaca aaaaatatag tgattatttt tatatataat cttaatcaat
     1981 gagaagtaag aagctataga taatgcttat atcatttatg gcttcttatt ttttatgtaa
     2041 tactcaaatt tttatagtat tctcttatta ttaaacagct tctgcctaga atattccata
     2101 tatatatgac ttctttcata aaacttgaac tgcccataac taaagtcaca agtatacata
     2161 actatttttt aatcaattat aaccaatatt tattttattt atcttcattt taattcttca
     2221 ttcaataatg taataagatt attttttatt tggatactat tatatcatct aaatatgtta
     2281 ttatatattt ggatattttc atattaatag gagggattct acaatgataa attatatggt
     2341 agaaattata tatagcgaaa aaataagcga agtagaaaac atgttgaaaa ttgaaaatat
     2401 acaatatgaa tttacttctc gtgatggctt tatatttttt ggtttttatg agatgacaga
     2461 tgcatataga tttgagactt tatgttcaga tgaaaaaatt cgatttaaaa gaatataata
     2521 aataaaaaaa gtacatattt ctaaactatt tgtttttcaa atttaggttg tgttttaatg
     2581 tagtgttgat atcataaaca attttaataa taaaaaagta ggaatattat attaatatgc
     2641 tcctactttt tactcatatt tattaatatt atttatcaaa actaccctac aaactggaat
     2701 ttatcattct ttcataattt taaactatca tatttaaaat atataaaatt ctacttgatt
     2761 tttgtataaa cgtaatatca atcattatcc aatttaaaag tattttttag taatttccat
     2821 ttcattttct tttaacatat
//
LOCUS       DAELXX010000216         2803 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.219, whole genome shotgun sequence.
ACCESSION   DAELXX010000216 DAELXX010000000
VERSION     DAELXX010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.219"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(119..280)
                     /locus_tag="KPH29_003646"
     CDS             complement(119..280)
                     /locus_tag="KPH29_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003489153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1469204.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARYFD"
     gene            complement(454..1320)
                     /locus_tag="KPH29_003647"
     CDS             complement(454..1320)
                     /locus_tag="KPH29_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG1469205.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNVEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTREYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPS"
     gene            complement(1546..2415)
                     /locus_tag="KPH29_003648"
     CDS             complement(1546..2415)
                     /locus_tag="KPH29_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG1469206.1"
                     /translation="MDYKTIILNRKSVREYKNIDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNKSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
BASE COUNT          925 a          494 c          295 g         1089 t
ORIGIN      
        1 tagcaattca agtagtattg gataggttct atcctatctc tacatataag tttccctata
       61 tgctgactat tttagctact aactcatgac taaagtcaca agtgtgcgta gctatttttt
      121 aatcaaagta tctagctatc ttaaaggcta agatgataga aggattatac ctaccatttt
      181 ctaatgaacc tatagtttgt cttgatactt gcaaaataga agcaagttct tcttgtttta
      241 tgcctctttc ttttcttatt tcttctaatc tatttttcac atctatcccc ctttaatatc
      301 tcaactacgg aaagtctact ttccataatt atacaactat atttcattta tgtaaagctt
      361 gctttccata tttacaaaaa aataacagac tattttcagc ctgtaatttt aaaatttcat
      421 gttgtttatt aaatttaatg ttattcatta actttaactt ggtttctgaa ttggaatcca
      481 aacttctgta gtataatcag gagcattgat atcgccattg ggataaagtt caatatctgg
      541 taagttagca tacacatacc cagaacttgg taaccactca gtaataattc ttttttgcaa
      601 ttcctgtatt gccagagcat ttggcattgg agaaacacat tcaaatactg cccatgtacc
      661 tgatggtatt aagtattcac gagtatcttc taatacaggc ttatctgttg gtgcagcaat
      721 ataataatct aaacccttac tatttgtaca cacactaact ccaagcaatc cacaaggttc
      781 gttatttatt aactctgaaa gtttatcaat tgttccattt tttatgcatt tagcccataa
      841 cttaggaact ttctcaaagc actcttctac aatcatgttc atactttctt ttataccaac
      901 aatacgaaac cctttctttt ctacaatacg atattgcatt tctacatttc cttttacata
      961 aatgcttaat gtcatcttag gatatgcttt taaaacaaca ccctttgctc tagctgcaga
     1021 tggagaaatg ttatgtatat tttgaaaagc tctattaaaa gatgttggtg aatcgtaacc
     1081 atacttctca gatagttcta aaattttgat gttactgttc tgaagttcaa atgcagcctt
     1141 agtcatacgt cttcgtctaa tatattcaga taatggtact tctgcaatat atgaaaacat
     1201 tctttgaaaa tgaaaggttg aacaacatgc aatttttgct gcttctgcat aatccactcc
     1261 actttcgaga ttttcttcaa tataagtaat agcttgattt aatttattta tccattccat
     1321 ttgatattac ctccaagcaa attatacagt ttaataaaat aatatacctc tctattcatg
     1381 cacaaaattg taagcattta aattcataag tatttttcaa tcaatacaat aaatataaaa
     1441 attgtattat acaataaaaa agttcaataa cattttatta taaaatatta ttgaactttt
     1501 tataaaaatt aatttaagtg tagttaaatt aagtattaaa ctatcttata tattacaata
     1561 tcctactatc ttatagtctt ctggcacttt ataatctttg tctggagtac ctaaattcca
     1621 ttttaaatca aacatagttg cacttattat tgttgcaaaa taaagcataa ctataccaat
     1681 gtctattttt tcttcatata aattagtatg accatctttt ctaacagcta aaactacagt
     1741 gcctccatca actataaatc tccatggttg tctatttaat gtagatggag ctaatcttgc
     1801 atagtgaaat gcatcaagaa gtgctctttc ttctaagatt tctatacttg caacatttcc
     1861 ccattcgttc atataaacaa catcttctac acctagtcta gtagacccct tatctgttac
     1921 aacttgcata tcagattttg aataattttg cccagttttt gtagcattta atactttagc
     1981 attacttaca ttttctccat atccaatagc tataattcct gttacctctt tatctgaatt
     2041 aattccaagt ttttctttta ccttttttcc atcttcaaaa gtaacccaac aagaaccaat
     2101 tccaagttct gttactttaa agattatact ttctcctatg taccctgaat tttcaatgta
     2161 attatctttt atgtcagata atattataat ataacttgga gaatctatca tatttccttt
     2221 gtaccctgct actttatcta aattattaaa aacttcactt ttattcataa ttcttacatc
     2281 aacatctatt tctggaacta gtttcttaca agattctgag tatttcttaa tttcactaag
     2341 atattcttct ttaatatcta tgttcttata ttctctaaca gactttctat ttaatatgat
     2401 tgttttataa tccataattt tatcctcccc aaactagtat tgtcttaatt atatacccat
     2461 taatatatta ttttactcat ttatttaata aataaagatt acaattttgt aattattatt
     2521 tattaataat attccacttt tacattaata atgaccagtt gtgcaaaatc cattgccaac
     2581 caccaaataa aaacatatta taaaagtaat ataaaataac aaacaaatct taacaaaaaa
     2641 ttaaactttt tttacacgaa agaaggcttc ccgttgcctt ctttattttt ataaaaaaat
     2701 aagctgtttc taaaatgatt tcacattttt gaaacagcca agttattcta taatgacaac
     2761 tataatttga ggatgtgagg gagagttgtc attataaatt tag
//
LOCUS       DAELXX010000217         2780 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.220, whole genome shotgun sequence.
ACCESSION   DAELXX010000217 DAELXX010000000
VERSION     DAELXX010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.220"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            1005..1394
                     /locus_tag="KPH29_003649"
     CDS             1005..1394
                     /locus_tag="KPH29_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG1469207.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
     gene            2139..2609
                     /locus_tag="KPH29_003650"
     CDS             2139..2609
                     /locus_tag="KPH29_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBG1469208.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIEFVKQCENSKE"
BASE COUNT         1270 a          169 c          482 g          859 t
ORIGIN      
        1 ataatataat taatgaaaaa atttaaattt tgtaatatat aaataaaaaa actctaaaat
       61 agaaggatat aattatatag aatgtttcaa ttaaacaagg caaaaactat aatcttaaaa
      121 ttaaaattaa cattattctt aagaagtaag aaatttaaga agtaaaaagt ttagtgataa
      181 gtggtagtaa gcaagaaatt tagaaagtag gaagtttagt gataagtggt agtaagcgag
      241 aaatttagaa agtgggaagt ttagtaataa tttgtagcaa gtaagaaatt taggaagtag
      301 gaagtttagt aataatttgt agcaagtaag aaatttaggg agtaggaagt ttagtagtaa
      361 taatttgtag caagtaagaa atttagggag taggaagttt agtaataatt tgtagtaagt
      421 aagaaattta gaaagtagga aatttagtaa taatttatag taagtaagaa atttagaaag
      481 taggaagttt agtaataatt tatagcaagt aagaaattta gaaagtagga agtttagtag
      541 taatttatag taagtaagaa atttagtaag tgagaagttt agttataatt tatagtaagt
      601 aagaaattta gtaagtgaga agttcagtaa taagttgtag taaatgaaaa gtttaggaag
      661 taggaagttc agtaataagt tgtagtaaat aaaaaattta ggaagtagga tttactgaaa
      721 tacaaatatt taaaatcaaa gagtttaaaa gaaaggagag ctactggaca agtatccaaa
      781 atagatagct gagtacaaaa tatctcataa aattaagcaa tataatacaa gagaattttg
      841 acattattta tatatattta ccgtgtacaa atagctaaat ttaggattaa gattgaaaaa
      901 tatcttataa gtctgagctt atataaatcc gtataagatg tatttaatcg aaaaatttta
      961 ttatggatat tgcttgtcta tattctgagt agcaaggttt tatcatgact ataaaaaagt
     1021 tgccacaatc agaattaaag ataatgaaat ttatatggaa atcagattcc aaagtaacat
     1081 ctagagatat tgttttagga atggagcaaa aatatcaatg gaaacaaacg acaactttga
     1141 ctcttttatc tagattagta gtaaaaagat ttctaaattc tcaaaagata gataaatata
     1201 cacattatga agttttaatt aaagaaaagg aatacatagg agttgagaca agagattttt
     1261 ttagaaatat tcatgattct tctataaaaa gtttattgtt gtcattacat gaaaatatta
     1321 atttgagcaa agatgatatc ttacttattg aagagtggat aaaaaatcta aaagaagagg
     1381 agaaagacgt ataagcaaac gtatttctta tagcattaat ataaagtgta tgtaagtgta
     1441 tttatgtaat atattagtct acttaagtaa tatgacaaat gattataagt ggattatgat
     1501 actataatgt gaatctaaat aaattaaata taaattgata gtggatattc tatttaatta
     1561 aatttaaaaa gctaccttta cttataagta taattaaggt agctttttaa atagaggtag
     1621 tgtgattaaa gagaattttt aaatataaat tgtgaagctt taatatagat gacaatatat
     1681 attttgcaaa tatgacacag tgttttcgta tttaagatga taaaataaat aggaattaat
     1741 gaagatattt aaaaaataaa aataaagaat taatagaaga tattaaagag atagagataa
     1801 aagtgttaat agaagatatt gaaaaagtaa aaataaagga ttaatagaag atactgaaga
     1861 ggtagaaaca aaagtgttaa tagaagatat tgaaaaaata aaaacaaaag attaataaaa
     1921 ggtactgaag agatagagat aaaagtgtta atagaagata ttgaaaaata aaaataaaag
     1981 attgatataa tatactgaag aggtagaaac aaaagtgtta atagaagata ctaaaaaagt
     2041 aaaaataaag gactaataaa aggtactgaa gagatagaga aaaaaatgtt aatagaagat
     2101 actgaaaaaa tagaaataaa aaattaggag gaaaatttat gaaatatcag gtttcattat
     2161 tagcagtgaa ggatattgaa gtatcaaaaa aattttacaa agaattattt gagcaagaaa
     2221 ttgaattgga cttaggcaaa aatgtaacat ttaaaggagg atttgctata caagaagact
     2281 tcgcatggct tacagggatt aatccagata caattataca aaaatcaaat aatatggaac
     2341 tttattttga ggttgatgat tttgacgaat ttattgaaaa actcaattat tataaagatg
     2401 tagagtttgt ccataagcca ctaaaacatg aatggcaaca aagagtagtg cgtatttatg
     2461 acccagataa gcatattatt gagataggag agtctatgga agttatagct agaagatatt
     2521 tatctgaggg atactctgta gaagaaactt ctaaaataat tcaacatcca attgaatttg
     2581 taaagcagtg tgaaaatagt aaggaatgat tgtttaaatt ataattattt agtagaaatt
     2641 aattgtagtg gaactaattc aagataatat ataggactag tatttgacaa atgtcataaa
     2701 ttaaattaaa ataaagaaaa tattaataaa gaaaaataag gggaatatta ataaagaaaa
     2761 aataaatata atattaaaat
//
LOCUS       DAELXX010000218         2743 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.221, whole genome shotgun sequence.
ACCESSION   DAELXX010000218 DAELXX010000000
VERSION     DAELXX010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.221"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..320
                     /locus_tag="KPH29_003651"
     CDS             <1..320
                     /locus_tag="KPH29_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG1469209.1"
                     /translation="IEDLQVKNMIKNRRLSRLISDVSWSEFVRQLEYKANWYGRKIVK
                     VGKFFASSQICNKCGYKNEEVKDLNIREWICPSCNETHNRDINASINILKEGLRLITI
                     QNK"
     gene            385..627
                     /locus_tag="KPH29_003652"
     CDS             385..627
                     /locus_tag="KPH29_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBG1469210.1"
                     /translation="MQNSMKQEPCDFSHGRFRFKTSDNRVLIPVVIFLGSIVTALCDL
                     ISRVILSPVELPISTITSLLGAPIVVMLLVKRKDRA"
     gene            630..1826
                     /locus_tag="KPH29_003653"
     CDS             630..1826
                     /locus_tag="KPH29_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG1469211.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFDLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDSKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(1946..2671)
                     /locus_tag="KPH29_003654"
     CDS             complement(1946..2671)
                     /locus_tag="KPH29_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBG1469212.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENMDLRGY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGANFRFCDLS
                     NSDIRGANLENSNLEHATLNGVISDESTPFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSIDGKESFKSARAYADENFIYRVGETIVAEDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
BASE COUNT         1051 a          315 c          452 g          925 t
ORIGIN      
        1 gcatagaaga tttacaagta aagaatatga ttaaaaatcg tagactatct cgcttaattt
       61 ctgatgtatc atggtcagaa tttgttcgtc aattagaata caaggctaat tggtatggta
      121 gaaaaattgt aaaggtaggt aaattctttg caagttcaca aatatgcaat aaatgtgggt
      181 ataaaaacga ggaagtgaag gacttaaata taagagaatg gatttgtcct agttgtaatg
      241 aaactcacaa tagagatata aatgcaagta taaatatatt aaaagaagga ttaagactaa
      301 taacaattca aaataaataa cttatgtaag aaccgtagga actatgggga tggcctgtgg
      361 agaattagta agacatattt tagtatgcaa aattctatga agcaggaacc ctgtgacttt
      421 agtcatggga ggttcagatt taaaacatca gataatagag ttttgatacc agtagtaata
      481 tttttaggca gtatagttac agctctttgt gatttaatat caagagtaat tttatcacca
      541 gtagaattac caataagcac gataacatct ttattaggag ctccaattgt agtaatgtta
      601 ttggtgaaaa ggaaggatag ggcttagaca tgttgaaaac taataattta agtgtaggat
      661 acaataacaa ggtagttata agtaatataa atgtagaagt aaaaaatgga gaaatactat
      721 gtcttttggg aagtaatgga gctggaaaaa ctaccctatt aaggtcctta tctaaactta
      781 tatcaccaat aaaaggtgaa atatatttga atggtgtaaa cataaattgt atatcaagaa
      841 aagcattatc aaaaaaaatg gctttagtac ttacaaatag attattaggt gatttaatga
      901 ctgtacaaga tatagtcaat ataggaaggt atccatatac aggatttttt gggagtctat
      961 ctaagaaaga tttaataatg gtagatgaag cattggagtc tgtagatgcc ttacatttaa
     1021 agaaaagata ttttgatgaa ctaagtgatg gagaaaaaca aaaggtgttg gttgcaagag
     1081 ctcttgttca agaaccagaa ataattatat tagatgagcc tacaactcac ttggatataa
     1141 aacatagatt ggagctcata aacatactga aaaaattaag taaagaaaag tcaatatcag
     1201 taatactttc attgcatgaa atagacatag cattaaaaag ttgtgataaa gttgctctta
     1261 taaaaaataa taaagtcata gcttatggtc agcctgaaga tgtagtagat gaggatatta
     1321 taaattcgct ttatgaattg gatgataaaa actttaatag tcttttaggt tcagtagaaa
     1381 ttagtaataa atctaaaaat gaagttttta taattggtgg tgggggtaaa gcaacaccaa
     1441 tttatagagc ttttactaaa aaaggtatag gtctgtattc tggaataata catgaaaatg
     1501 atattgatta tgaaattggt agaactatgg ggataaagat gtttacagaa aatccatttg
     1561 agccgataag tgatgagagt ttcgatttgg ctattagaaa tttaaatgat tccaagatta
     1621 taatagatac aggatttagt gtaggagaga caaataagag gaatatagat attataaaag
     1681 aggctttaaa actggataaa aaagtttatt cttttagaaa tagagatgag agtaagaagt
     1741 attatgacag tttggatagt aagatagaac atatagataa agtttctcaa ataatcaata
     1801 gtgcagatat aaataattta ttataattta tgaaataaaa tgtacttatg tgctatagaa
     1861 aaaagagtat agattataat ttttttgaaa ttacaatcta tactcttttg ttattttgtt
     1921 tatatattta taaaacttta gcattttata gataaccaat agcttcctct ctagttaaaa
     1981 aaaagtgaat ccctgtagtc gattctttcc atctattttc attaaaatct tcagctacaa
     2041 tcgtttctcc tactctatat ataaaatttt catcagcata tgcacgagca cttttaaaag
     2101 attctttacc atctatacta gtaattgaca gtacttttgc cttatcacat ctacaagcat
     2161 ttgatgtagc agacactctt tttgcgtcgc tgggtattaa taattgtacc attctgaaat
     2221 taaaacattt tttatagcct ataaatgctc ctgtttcagg acaatacaat ttaaaaaaag
     2281 gggtactttc atctgatatt actccattta aagtagcatg ttctaaattg gaattttcta
     2341 aattagctcc tctaatatct gagttagata agtcacaaaa tctaaaattt gcaccactta
     2401 agtctgcatt ttttaatata gaatttttta atgaacaatc cgaaagataa gaattattaa
     2461 aaatagagtt ttctaaatct gctttttcta aatttaaatt gataaggtca gaccacgaaa
     2521 agtcaatatt acttaagcag tatcctctta agtccatatt ttctaatctt aattctttta
     2581 aatctgcctt ttctccagca ttattgtttt taagccataa ataatgtttc tcaagtgttt
     2641 tttctaattc ttgtttagaa atagttttca ttaaaatcac cttcataaat ttatttttat
     2701 atatgtagta gcaaaatagt catatattta attatataac aat
//
LOCUS       DAELXX010000219         2685 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.222, whole genome shotgun sequence.
ACCESSION   DAELXX010000219 DAELXX010000000
VERSION     DAELXX010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.222"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            37..1269
                     /locus_tag="KPH29_003655"
     CDS             37..1269
                     /locus_tag="KPH29_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1469213.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKLGDEIALELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            1419..1736
                     /gene="trxA"
                     /locus_tag="KPH29_003656"
     CDS             1419..1736
                     /gene="trxA"
                     /locus_tag="KPH29_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBG1469214.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            1753..2631
                     /locus_tag="KPH29_003657"
     CDS             1753..2631
                     /locus_tag="KPH29_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG1469215.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
BASE COUNT         1086 a          266 c          472 g          861 t
ORIGIN      
        1 atattaaaac taaacgtaag gtaggatata tattcgatga taaataagaa tgtatttacc
       61 agtactaaaa atcatttaat aaaaatgtat attattgtag taggtagttt tttaataatc
      121 ttttctatat ttatatactc atattttagg ggacttacat atagtggaat tgatagtgag
      181 ataaatgatg aacttgaata tatagtctct caatttaaaa gaacttcatt tttgaaccca
      241 ataagactga aagatcctaa agatatggtg tatgtatatg aagatggaag gataagttac
      301 tatactcaaa atgaatattt tgatgagtta cttccagata gaagactaga taaaaaaaat
      361 tcgtttttta aatacacaga aaatggatat acttttagag aattaaatgt agatgtaggt
      421 agataccaaa tacagataat tagaaatata gattctgaga tgaattcttt aagacagctt
      481 acatctgttc ttataattgg aatattaatt tctgtaataa ttacttactt tgtggctgta
      541 tatctcacta gaaaagcttt aattccaata gaaacagcat ggaaaaatca agctaagttt
      601 atacaagatg cttctcatga attaagaaca ccaataacaa tagtttcatc aaaattagaa
      661 agtatgttaa aatccccaga aagtacagta aatgatgaag ttgaaacaat agctactgca
      721 atgaaggaaa ctagaagact taaaaaaatg ataactgatt tattgtctct tactaaagag
      781 gactctattg ttaaagttaa tttagaagaa atagacttag aaaaattatt ggaagaaata
      841 tcagaagact atattgacat tgctgagttt caggaaaaaa gattcgtgtt taattctaaa
      901 ctgaagaata aagttataat aacagataaa aataaattaa gacagcttat attaattttc
      961 attgataatg cctttaaata tacaaaattg ggtgatgaga tagcattaga attaaaagaa
     1021 gatatagagg atgaggtaac tttacttata agtgacactg gtataggcat aaaaaaagaa
     1081 gagatacctc ttatatttga tagatttttt agaagtgaaa atgtaagaaa taaagattta
     1141 gaaggttctg gaataggact ttcaatagct cgcatgatta gtttaaactt atctattgat
     1201 ataaatgtaa cttctgatgt agatattgga actacatttg aattgtcaat accaaaaaag
     1261 ttaaaataat aaaaatataa aaaattgttt ttagaaatga agaatgggta tactaattag
     1321 tataaaatat aaaaagacct tataaaagta aatttatatt tataaatttc atagtataat
     1381 tatataggga taataaagtt taataggagg atttaaaaat ggcaaaagtt ataaatacat
     1441 ctgaatttag aagtagtgtt gaaggtagta agggagtagt tgtagttgat ttctttgcaa
     1501 cttggtgtgg accttgtaac atgttgggac ctgtatttgc agaattaggt gaagagatga
     1561 aggataaagc tagatttgta aaagttgata tagatgagag cttagaaata gctcaacaat
     1621 tcaatgtatc tacagtacca actatgatta tatttaaaga tggaaaacca gtagaaactt
     1681 taattggatt catgccaaag aacaaaattg aaatgcaagt aaaatcatat ttataaaaaa
     1741 tgggtgagta ttatgagata tgatatagct ataatcggca gtggtccagc tggattgtct
     1801 gctgctataa atgcaaaaat aagaaataaa acaattatca tgtttggtaa tgataattta
     1861 agtaataagc ttgttaaagc tccttcaata gataattatt taggttttta tgatataagt
     1921 ggtgatgaat taaaagacaa gtttaaatca catatagata gtatggatat ttcaattgaa
     1981 aataaaagaa taaataatat ttatgctatg ggagaatact ttacaataat gtctggaaat
     2041 gatatgtatg aagctactac tgtaatcctt gcaactgggg tagagtatac tagaccaatc
     2101 aagggtgaag aagaatttct aggaagaggt gtaggctact gtgctacatg tgatgcacca
     2161 ctttatagaa ataaaaaggt agcagttata ggatataatg aagaatctaa agaagaagca
     2221 aattttttaa gtgaactcac ttcaaagact tattttatac ctatgtacaa aaaagataat
     2281 cttatgagaa gttcagataa cctagatgat tctattgaag ttatacatga cagaccagta
     2341 cagatagatg gagataaact tgtaaacaaa gtatctttta aagaaaacca cattgaagtt
     2401 gatggagtat tcgttataaa agatagtacg gctccaagtg cattggttcc tggaatagag
     2461 attgatggca ttcatattaa agttgacaat aatatgaaga ctagtataga tggatgcttt
     2521 gctgctggag attgtgttgg aaaaccctat tcttatataa aagcagctgg tcaagggcaa
     2581 atagcagctt taaatgctgt ttactattta gataaattaa agagggctta atatttatat
     2641 tgattaactt tttaaagata tttataaaaa actgtcttga aataa
//
LOCUS       DAELXX010000220         2566 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.223, whole genome shotgun sequence.
ACCESSION   DAELXX010000220 DAELXX010000000
VERSION     DAELXX010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.223"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..679
                     /locus_tag="KPH29_003658"
     CDS             <1..679
                     /locus_tag="KPH29_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBG1469216.1"
                     /translation="IKDKYKDEIDKSEYKYANYKSDLLRIYNITKSRNDKQKNHQEIC
                     IIFSILENGLQENIRDGVTASKKFLEYKSIQYKNNESEKLELLKDKFDEFNRMILKIS
                     EKIEYPFSTSLISDDKQRQSKYLIGVSIVLASICYYYDIDLENNYLIQDIKKIISCSP
                     IADIEYKASSTNMKNMILYLFIKNKIQDNKFESLNLKRDKIKEINTLVMNNDGYLNEQ
                     TRLLEIY"
     gene            complement(906..1091)
                     /locus_tag="KPH29_003659"
     CDS             complement(906..1091)
                     /locus_tag="KPH29_003659"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBG1469217.1"
                     /translation="MEGFWRILKVEMYYLRKLDTYEQLVKTIEDYIYYYNNFRYQKRL
                     NSMSPLEFRKYLNTEIA"
     gene            complement(1121..1510)
                     /locus_tag="KPH29_003660"
     CDS             complement(1121..1510)
                     /locus_tag="KPH29_003660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HBG1469218.1"
                     /translation="MRAVGLSSVCRKKKFSYVKCTPEVIAENVLSRKFSADKTSQKWL
                     TGVTEFKLTNGMKAYLSAILDLDDRSIVSYVIGKSNNNNLVLETFDKAIELYPNAKPI
                     FHNDKSFQYTSKVFKSKLLTQGMIQSM"
     gene            complement(1522..1982)
                     /locus_tag="KPH29_003661"
                     /pseudo
     CDS             complement(1522..1982)
                     /locus_tag="KPH29_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888992.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          980 a          339 c          305 g          942 t
ORIGIN      
        1 aataaaagat aaatataagg atgaaataga taaatcagaa tataaatatg ctaattacaa
       61 atcagattta cttagaatat ataacataac taaatctcgt aatgataaac aaaaaaatca
      121 tcaagaaata tgtataatat ttagtatttt agaaaatggg ctacaagaaa atatcagaga
      181 tggagttaca gcctctaaga aatttttaga gtataagtca attcagtata aaaataatga
      241 aagtgaaaaa ttagagttat taaaagataa gtttgatgag tttaatagga tgatactgaa
      301 aatatctgag aagatagaat atccattctc aacaagttta atatctgatg ataagcaaag
      361 acaaagtaaa tacttgatag gagtatctat tgtgttggct tctatttgtt attattatga
      421 tatagacttg gaaaataatt atttaataca agatattaaa aaaataatat cttgttcacc
      481 catagcagat atagaatata aggcgtcttc aactaatatg aaaaatatga ttctatactt
      541 atttataaag aataaaatac aagataataa atttgaatca ttaaatttaa aaagagataa
      601 aattaaagaa ataaatactt tagtaatgaa taatgatggt tatttaaatg aacagacaag
      661 gctattagaa atatattaac aagttataaa ataaatcatg gtaatgtaaa ataatttgtg
      721 cttttaaggt taataaaaat cttctttatg gcaattattt aaatataatc aaatgtcgta
      781 aaggagattt tttatgaacg ctttaagaaa acaattagcc aaagaaatta tatcagatat
      841 ctaaaaaaga gcatgtcaaa taaaacccta ggtatttaaa tttttttaga gctacttaaa
      901 ataaattatg ctatctctgt atttaaatat ttcctaaatt caagtggaga catagaattt
      961 aatctctttt gatatctaaa attattatag taatatatat aatcctctat tgttttgact
     1021 aattgttcgt atgtatcaag ttttcttaaa tagtacattt ccacttttaa aattctccaa
     1081 aatccttcca taggagcatt atcaatacaa cgagcaactc ttacatactt tgtatcatgc
     1141 cttgtgttaa aagttttgat ttaaatacct tactagtata ctggaaactt ttatcattat
     1201 gaaaaatagg ttttgcattt ggatatagct ctattgcttt atcaaaagtt tctaacacca
     1261 agttattgtt atttgacttt ccaataacat atgaaactat acttctatcg tctaaatcta
     1321 gaattgcgct taaataggcc ttcatcccat tggttaattt aaattcagtt acacctgtta
     1381 accatttttg agaggtttta tctgcactaa attttctact taatacattt tccgcgatca
     1441 cttctggcgt acatttaaca taactaaatt tcttttttct gcaaacagaa cttaagccta
     1501 cggctctcat tagtctgtac actctctaca atctttaaca atttcaactc tttcatctaa
     1561 tgctgttttt ctagtctcca taaagatttc tcccttcgag gtttctttta tctacttatg
     1621 aggattatac tctttacccc aagcttttag tgaataaact gatctcattt tatatttttt
     1681 tgagattgtg tcataagatc cttcaccatt tagataagat tttatagcat tcaatttaaa
     1741 ttcttcacaa tagcataatt tacaactttg ttttattaaa ccatcatttc caaaagcttc
     1801 ataatttcgc atccactctc tgaatgtacg tttatccaat atttaattct tccattattt
     1861 ttattactgt attctcacct tttaaatatg attcaattgc ttcgattttt ttgctctgaa
     1921 gatatcttag ttttagatag tcttacatat tttaactgtc tcctaagatg agtataattc
     1981 attcatcatc agttctatag aatacaccta gttcctactt actgtaggaa ctaggactat
     2041 atgaaaccca ttcatagatt gtagtttctt ctgttgcatt atagcgcaga atagtaaatt
     2101 catgtgaaat tttattttga atttactatt tatatataaa tgaagagtgg tcttattttc
     2161 aattaaaatt tggaacaatt ttgggttgac atctacaatt aaggatttaa gtttttgtta
     2221 tactaataaa taaaaacaga gattcaaaat atataaataa tacttaaact agtgctgata
     2281 gattttcttt aagttctagt ttttttattg tcattatcta atatgtacta ttttatgtac
     2341 agtcaatata atctgaaatt gtaaaatggt gtataatata gttataagta aagaagggat
     2401 ttaaacatgt atgattacaa aaataaaaaa gacttatata taccttaagg aaatacaaaa
     2461 aatatacaaa atcaatgagc ataaagaact agtacacata ataaaaaaac taataaatga
     2521 taataaaata aaaccaataa aaacctcaga tctaacacct cagcat
//
LOCUS       DAELXX010000221         2539 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.224, whole genome shotgun sequence.
ACCESSION   DAELXX010000221 DAELXX010000000
VERSION     DAELXX010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.224"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(205..1455)
                     /locus_tag="KPH29_003662"
     CDS             complement(205..1455)
                     /locus_tag="KPH29_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HBG1469219.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLYEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDSDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1487..2428)
                     /locus_tag="KPH29_003663"
     CDS             complement(1487..2428)
                     /locus_tag="KPH29_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HBG1469220.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
BASE COUNT          759 a          492 c          240 g         1048 t
ORIGIN      
        1 taaatataca aatacaatta tatgaatttt catcctcaat tatagcatag aatatgacta
       61 tctatatcct aaaattatcc atttctaaaa aaaggtaact attattaaga atatcaactt
      121 atagaattta tattccacat acgattttca atcaaaaaaa caagggctaa aaagtgaatt
      181 gtacactttt tagcccttta ttaattattc atttatttca tgtaagaata gcccacatct
      241 tactttaggt tcaaaccatg ttgacttagc tggcattatc ttatttgcat ctgctactct
      301 tattaaatct tcaatactag taggatacat tgcaaatgct actttcatat cttcattaac
      361 tcttttttct aattcttcaa gacctctaat tccaccgata aaatcaattc ttttatctac
      421 tctaggattg tcaattccta aaatagcatc aataacatag tcttgtaata tagaaatgtc
      481 taatgagtca acagggtctt ctatttctaa taaattttta tttgctttca ttttatacca
      541 cttatcacct aggtacattc cgaaagttcc ttttttgtca ggtttatatt tttcttttcc
      601 ttctatttct tctagttcaa aattttcttt tattttattt ataaattctt cctcgcttaa
      661 accatttaag tctttgataa ccctattata atccaaaacc attaaattct catctggtgc
      721 aatcattgct atgaagtaat taaattcttc tttgccagta taatttggat ttttagctct
      781 catttctttg gctacatttt ctgctgaagc tgtcctatga tgaccatctg caatgtatag
      841 ataatctaat ttttcaaatt tatctactag gttatctatt atatcatcac tatcaatctt
      901 ccatactgta tgctctatct tgtcatctgt tatgaaatca tataaaggtt cattattatc
      961 cataaaatca ttgattatat catctatcac cctttgatgt ttgtaagtta caagtatagt
     1021 tcccgtatta gcatcacagt attttatgtg tttagttcta tcaatttcct tttctggtcg
     1081 agtgtactca tgttttttta ttgttccatt caagctttca tcaactgata tacatgctac
     1141 tataccaact tgagctctac catccatgat ttgtttatat atatatatac actctttgtc
     1201 atcttttact agaatttctt gttttcgaaa ctcatataaa ttttctcgcg cttttaaata
     1261 aactttttca tcatataaat ctatattttc atctaaatca atttctgatt tatctatatg
     1321 caaaaatgaa tatggattcc cttctgcaat tcttctagct tctttactat tcataacatc
     1381 gtaaggaagt gctgctactt tatctactat atatttattt ggtctaatcg ccttaaatgg
     1441 tttaatcgtt gccattacta atttcctcct aattatacaa aatattttat aatgctaccc
     1501 ctattagatt gttgaagtca agaaagaaat cttgaatcac atttactatt tcgtctccta
     1561 ttctttcttg agcttctact gtagatgccc caatatgagg tgtaggagaa actcttggat
     1621 gatttactaa gtctaaattt aatgtaggtt cttgttcata aacatctata gcagctgctg
     1681 ataatttacc ttcatctaat gcttttagta aatctttttc atcaactaaa ccgcctcttg
     1741 cacagtttat taaataagcc cctttcttca tcttatttat ttctttttca gttattactg
     1801 cccccttatt cttatcaaat ggtatgtgta aagataagaa atctgcattt tctaaaactt
     1861 cttccatatc acagaattta taattgttaa aaccttgcgc ctcacccatt atgtcagtat
     1921 atataacatt catacctaat aattctgctc tctttgcaac ttcttttgca attcttccaa
     1981 aacctataag tccaagtgtc tttccattaa tttctgtacc tttatacttc ttcttctccc
     2041 attttccatc tctcatagtt acatttgcag tatttatgaa tcttgcaaga acaaataatt
     2101 gacctattgt aagctctgca acagatatac tgcttgcatt tggagtattc ataactttta
     2161 tgccattagc ttgagcatat ttaacatcta tattatccag acctactcca cctcttacta
     2221 tcaacttcaa ctttttaccc tctaatgctt tatctattac atctttagta actttagtag
     2281 ctgaacgtac tacaagtaca tcaacatctt taagtttatc tcctaaaaca tctttttcat
     2341 aaaactgctc tatcacatta aaatctaact ctctcaattt tctcgctgct tctttctcta
     2401 ttccatcagt aactaatata ttatacataa caaactcctc caatttttat tcacttaatt
     2461 tttccaattt ttatttttta aagtattttc atcaataaaa aactttactt aatttaactt
     2521 tctgtttata taattttca
//
LOCUS       DAELXX010000222         2504 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.225, whole genome shotgun sequence.
ACCESSION   DAELXX010000222 DAELXX010000000
VERSION     DAELXX010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.225"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            261..401
                     /locus_tag="KPH29_003664"
     CDS             261..401
                     /locus_tag="KPH29_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1469221.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(398..769)
                     /locus_tag="KPH29_003665"
     CDS             complement(398..769)
                     /locus_tag="KPH29_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1469222.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
     gene            1318..1575
                     /locus_tag="KPH29_003666"
     CDS             1318..1575
                     /locus_tag="KPH29_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1469223.1"
                     /translation="MIIIRSQDKTNLMQINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
     gene            complement(1685..2365)
                     /locus_tag="KPH29_003667"
     CDS             complement(1685..2365)
                     /locus_tag="KPH29_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG1469224.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVMIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSGLFRIDEINLIPFDSVGILTYILNIIMFMPLGFLL
                     PLIWKNFRNIINVSLTGLGFSLAIELCQLFNLRATDIDDLMMNTLGAVLGYFIWVGVN
                     NLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISKKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
BASE COUNT         1018 a          272 c          282 g          932 t
ORIGIN      
        1 aatcatcctg aaaaatatat aaaaatagaa aaaacataca agattctaca ataatagatt
       61 tttttgttat tattacgagt tttttattgc aactgtagaa taaaagtatt attatataat
      121 taagataatt atctagggaa attttggata atcaaaatat attttataaa atcaaaatac
      181 aattacataa actttattaa aaatttgttg ggaatttctg actagcatta agaacgtaag
      241 tttgttgggg gatggtatat gtggaaaagt caaacactac tgttacaaaa aaagaaaaaa
      301 ttgaagatat aggaatgaaa ttagaacatt tattgagtga agatattact ttttatgaat
      361 tattacttga gattttgtat aaagaaaaag aagagaatta attctcttct ttaaggaatc
      421 tttttaaata ggcattatca atcatttttt ctatagcttc tctatcttct ttttcaagag
      481 aatatatttt ttctaagagc ttttttgttt tttcacttac tgataagttc tctatgatta
      541 atttatcttc tttgcttatt tttgtattat catttacgcc taataaataa tctgttgatg
      601 tttctaatgc ttttgctaat ctaactatta tttcactttt aggttctctt aaattatttt
      661 cataacgtga caagctagcc tcagttatac cgactttagt agcaagttct ttttgattta
      721 tatctaattc ttttcgtaga tttgctattc tatctcctaa actttccata tgtacatcac
      781 tccttaccga aattataagt tattttgata aaaagtaaaa taaaattacc aaaaatataa
      841 aaaaaaaggg ttaaaataat aaaatatatt gaaaattacc atttgtatat gtataatatg
      901 aattatacaa atggtaattt gatgagaggt ggtatgttga aataagaaac tgaattaatt
      961 aataaatata tagaaagttt tgaaaaccct taattgagat ataaacaata tgtacaaaat
     1021 tttttgcaag taaaattacc aaatgtataa aataaatcag tagcatagat aattttatgc
     1081 tttaatacat tagattattt aatttagtta taatggtcaa aaatgcaatc tacaaataaa
     1141 acaaagtatt atcaaaaaag tattctttaa gagaattttt tattagagat gtaatttata
     1201 taaataatcg tattaaaaaa taaaatacta agtttaaaaa tttaaataat ctataaatca
     1261 attataaaat attatttctt agaaattaaa atgaattaag taaatggagg aattgatatg
     1321 ataataatta gaagtcaaga taaaactaat ttaatgcaga taaaccaaat taaaatagat
     1381 ggtagtcaag tttatgcagt atttgaaagt aaaattgata ctgtaaaaat aggagattat
     1441 gaaaataata caagagctat acaagtgttg gacaatatac aaaacttcat tgaaaatgga
     1501 acaaaatatg attatataac atcaaataaa gtcagataca atgttaataa aatatttcag
     1561 atgccagcta aatagtgagt gtgcatttta gtttaataac taaagcccac tttcttttgt
     1621 agtatgaaag agggctttac atatttgtaa atatcgaagt acacttgttt tatcaataaa
     1681 ttatttaata aggtataatg gaccaattaa acaataaaaa ttctcctaaa acagcaagta
     1741 ttaaatatat aatagcttca tttttgtaaa gagaggtaat ttttttgctt atgtcatcat
     1801 caattgtaat agcaatttca tcttgaataa tatttttata tatagatggg aaaatatctt
     1861 ttttctttat aagattaaat aaattattta cacctaccca tataaaatat cctaaaacag
     1921 cacctaatgt gttcatcatt aaatcatcaa tatctgtagc tcttaagtta aacaattgac
     1981 aaagttcaat tgctaatgaa aaacctaatc cagttaatga aacattgata atatttctaa
     2041 aatttttcca aattaaaggt agtaaaaatc ctaaaggcat aaacataatt atatttaaaa
     2101 tatatgttaa gatgccaact gagtcaaaag gtattagatt tatttcatca attctaaaaa
     2161 gcccactata ccttcctata tcccatatgc taccaattcc agctttacta agtgctagat
     2221 agatatataa taaaaaaata tatacccata ctaaatgtat cattacattc ttttcaactt
     2281 tcaaattttt tcgttttata acgactattt ggtatattaa gcatggtaat ataacgcaaa
     2341 taaatgaata aaatgtccat atcattttat tttgtcccct ttcaaattta tgtttttagt
     2401 tttataagta ctatctttat attaaactgt aagttttaaa aatcttttaa taaaatctta
     2461 aaaatttatt aatttataaa aatatattat taagattaaa aaat
//
LOCUS       DAELXX010000223         2469 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.226, whole genome shotgun sequence.
ACCESSION   DAELXX010000223 DAELXX010000000
VERSION     DAELXX010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.226"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            65..364
                     /locus_tag="KPH29_003668"
     CDS             65..364
                     /locus_tag="KPH29_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1469225.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSRKINWDKVSNIRFD"
     gene            688..2343
                     /gene="ilvD"
                     /locus_tag="KPH29_003669"
     CDS             688..2343
                     /gene="ilvD"
                     /locus_tag="KPH29_003669"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBG1469226.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELEELAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRIAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIEFNLDFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIRNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
BASE COUNT          957 a          271 c          475 g          766 t
ORIGIN      
        1 tgtataaata ttataaatat atggaaataa taatagtata ttttggtaaa gtaggaggaa
       61 ttttatgaaa tggattaaat ggtatagtat tacatgcata tgtattttta tagtagtagc
      121 tttttatatg tttatatttc ctaataaaat agaaactatt gatacttcat ctgcatatag
      181 ctttgttgaa aagaaagtac caaatagtgc tgtatatcaa ggatacaaaa agaatccagt
      241 tgatggtact actacaattt attacagcta tgataattca actcatatag ttagattatc
      301 acatccagaa gactattcaa ggaagattaa ttgggataaa gtttcaaaca tcagatttga
      361 ttaatcttta atatttataa aagtctatat atctaaggca aatatattat taatcttatt
      421 catagttgag cactttctat tatagaagtg cttcttctga tttatatcat aaaatattac
      481 tcaacaattt gtaaaatata ttagtttata aaatatattg aaaagataat attatataat
      541 acgattgtgt aagatattga taaaaaatcg taataaattt tcattagtat atgctaatat
      601 ttttttacaa ttttcgtaat taattaatag aaaaatatat tataataata ttatgaaaat
      661 ttaaaaaagt aagaaagagg taatattatg agaagtgata taaaaaaagg aatagaagga
      721 gctccaaaga gagcgttaat gtatggaatg ggtcttacta aagaagaaat tgaaagacca
      781 ttaataggta tagtaaatgc acaaaatgag gtgattcctg gtcatctgca tcttgatgaa
      841 atagcagaag ctgcaaaaaa tggagtaaga atgagtggtg gtttgccatt agaatttcct
      901 gctatagggg tatgtgatgg tatagctatg gggcatgtcg gaatgaatta ttccctggct
      961 tcaagagaat taatagcaga ttcaatagaa gctatggcta tggcacatgg ttttgatgct
     1021 ttggtactta tacctaattg tgacaagata gtgccaggta tgttaatggc tgctgcaaga
     1081 cttaatatac caagtattgt tgtgagtgga gggcctatgc ttccaggtaa aaaaaatgga
     1141 aaagtatatg attttaactc tgcaatggag ggtgttggag catgtaaaga tggaacagta
     1201 agtgaagaag aattagaaga gttagccatg aactcttgtc ctggatgtgg ttcatgctct
     1261 ggacttttca cagcaaatag catgaattgt ttaacagaag ctttaggcat gggtatacca
     1321 tataatggta ctgctgcgtc acattctgga gaaagaaaaa gaatagcaaa atatgcaggt
     1381 atgtatgtta tggagttact taagaacgac ataaaaccta gagatatttt aacaatagat
     1441 gcttttaaaa atgctatagc tgttgatatg gcaatggctg gttctacaaa tacagtactt
     1501 cacttacctg caatagctta tgaatcagga atagagttta acttagattt ttttgatgaa
     1561 ataagtgaaa aaactccttg tttaacaaaa ttaagtccaa gtggaaaaca tcatattgaa
     1621 gatttacata tggcaggagg aataccagct ataatgaacg aactttcaaa gataaatgga
     1681 ataaatttag attgcaaaac cgtaacaggc aagactatag gggaaaatat aagaaattgt
     1741 gaaatagaaa atgaagaagt aatacataca ttaaagaatc catatagtaa ccaaggtggg
     1801 cttgcaatat tgaaaggaaa tcttgctcta aatggagctg ttgtaaaaaa gtcagcagtt
     1861 gcagaagaaa tgttagttca tgaaggacca gcaagagttt ttaattcaga agaagaagct
     1921 gtaaatgcta tttttggtaa aaaaataaac aaaggtgatg ttatagttat aagatatgaa
     1981 ggtccaaagg gtggtccagg aatgaaagaa atgctatctc ctacatcagc agttgcagga
     2041 atgggacttg ataagcatgt agcacttctt actgatggtc gtttttcagg agcaactaga
     2101 ggagcatcta taggccatat ttctccagaa gctatggaag gtggtttaat cggactagtt
     2161 gaagaaggag atataatttc tataaacata ccagacaaaa aattagagtt aaaagtagat
     2221 gaagttgaaa tagaaaatag aaaattaaaa tttaaacctt tagaaccgaa aataaagcat
     2281 ggatacttaa gtagatatgc taaattggta acatcagcaa atacaggagc agttttaaaa
     2341 tagtgaacta tatttacgaa aattagaaaa aattaagaaa atattaattg gatatcttag
     2401 gtgttatagc actaatgagg tatccttttt attttgtatg aaaaatatac tgtttgtata
     2461 caaaaatat
//
LOCUS       DAELXX010000224         2435 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.227, whole genome shotgun sequence.
ACCESSION   DAELXX010000224 DAELXX010000000
VERSION     DAELXX010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.227"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1790
                     /locus_tag="KPH29_003670"
     CDS             <1..1790
                     /locus_tag="KPH29_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG1469227.1"
                     /translation="IYSAIWLVIIIFFIFSLYHSIEHIKIISHTGTIRSETQKVVKQE
                     LNNERNDDLIKRLDNILIKLRTGNGENGFQRCDNKEFQQKLNQMDSMWESMKKEIIKV
                     RNGASGDKLYKLSEEYSVLSNQIVFISEKHSNAKLYSFATALFIYLIFSTISLLIWEY
                     YNKKRFKRIFYTDNLTKIKNQVAFENRAIEILYNASNKEYVLLNIDIDNFKYINDTHG
                     YEYGDKVLIIVAAALSKTFNIKETCARIGSDNFVILAKYRDSLLEDIREMLTNAIISE
                     LDMNVTQTISYCIGAYLVEIDNLGYKSINSIMDKANIAHKVSKTRGISSTVWYNENLL
                     KQLQMENSIYNYMYKAIENEEFHMYLQPKFQISSLNVVSAEALVRWFSPELGFLSPDE
                     FIPLFEKSGFIIELDFYMLKKACSFVKKTFMKKNQYTYPIAVNFSRVTIYQNSFYQRF
                     LDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFGSGYSSLSL
                     LCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIETKKDLEFLN
                     TVKCDVGQGFYFSKPIEEKEFFNKYVTKK"
     gene            complement(1878..>2435)
                     /locus_tag="KPH29_003671"
     CDS             complement(1878..>2435)
                     /locus_tag="KPH29_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1469228.1"
                     /translation="QFERSYDIGIFKNEQSIYKFIEKIPFVKKDNSNFNYTNYYMEFE
                     DIPEYYEVNFNNYLYIITRFSFIPDGTEIFFTWEEIHYWDEKVATEKTFINGETLVDS
                     YNFPNNEVKDYIQKRESLYIETKKYYEEKGLKIERHALGSEDGEYVELTNDSILYLLD
                     IEAVEIREQSKNIEEFLYKYKKRFK"
BASE COUNT          894 a          272 c          348 g          921 t
ORIGIN      
        1 taatttattc tgctatttgg ctagttatta ttattttttt tatattttct ctatatcata
       61 gtatagagca tattaaaatt ataagtcaca ctggtactat tcgcagtgaa actcaaaagg
      121 ttgttaagca agaattgaat aatgaaagaa atgatgactt gataaaacga ctggataata
      181 ttctgataaa gctaagaaca ggtaatggag aaaatggatt tcaaagatgt gataataaag
      241 aatttcaaca aaaactaaat caaatggatt ctatgtggga atctatgaag aaagaaatta
      301 taaaagttag aaatggtgct tcaggtgata aattatataa attgagtgaa gagtattctg
      361 tgttgtctaa tcaaattgtt tttatatctg aaaaacactc taatgcaaaa ttgtattctt
      421 ttgctactgc actatttatt tatcttatat tctcaacaat aagtctttta atttgggaat
      481 attataataa aaaaaggttt aaaagaatat tttatactga taatttaaca aaaataaaaa
      541 atcaggttgc atttgaaaac agagcgattg aaattttgta caatgcatct aacaaagagt
      601 atgtactgct aaatattgat attgacaatt ttaaatatat taatgataca catggatatg
      661 aatatggaga taaagttcta ataatagttg cagctgcact ttctaaaact tttaatatca
      721 aagaaacttg tgcaagaatt ggttcagata attttgtaat acttgcaaaa tatagagatt
      781 ctcttttaga ggatattcgg gaaatgctaa ctaatgctat aatctctgaa ttagatatga
      841 atgtaactca aactatatct tattgtatag gagcgtactt agttgaaatt gataatttag
      901 gttataaatc catcaattct ataatggata aggcaaatat tgcacacaag gtaagtaaaa
      961 caagaggaat ttcctccaca gtatggtata atgaaaatct gttaaaacag ttacaaatgg
     1021 aaaatagtat ttataattat atgtataaag cgatagaaaa tgaagaattt catatgtatt
     1081 tgcaacctaa atttcaaata tcttctttaa atgttgtaag cgcagaagct cttgtaagat
     1141 ggttttctcc agaacttggt tttttatcac cagatgaatt tattccattg tttgaaaaaa
     1201 gtggatttat tattgaattg gacttttata tgttaaaaaa agcgtgttca tttgtaaaaa
     1261 aaacgtttat gaaaaaaaat caatatacat atccgattgc agttaatttt tcaagggtta
     1321 ctatttacca aaatagcttt tatcaaaggt ttttagatac tgttaaagaa tatgaaattc
     1381 cttttaagta tatagagata gaggtaactg aaagtgcttt taatgaaata tctcaaccag
     1441 ttatttcgat tttagaggag cttaaaaagt taggattttt aatttcgatg gatgattttg
     1501 gatcaggata ctcttcatta agtcttttat gttctctatc tattaatgga ttgaagttag
     1561 ataaaagctt acttaaagaa acatttaacc gggagaaagt atatagtatt attcagtgta
     1621 ttattgaaat gtcacatcgt ataggcatgt ctgttgtatg tgaaggtatt gaaactaaaa
     1681 aagacttgga atttttaaat acagttaaat gtgatgtagg acaagggttt tatttctcta
     1741 aaccgataga agaaaaagaa ttttttaaca aatatgtaac taaaaaataa tataattatt
     1801 tttatcagtc tatacattga gtcattttat atttcgttaa atgactcaat gtattgttat
     1861 gtattttaaa atataaatta tttaaaacgt tttttgtact tatatagaaa ttcttctata
     1921 ttttttgatt gttctcttat ttcaactgct tctatatcta gtaaataaag tatagaatca
     1981 tttgtaagtt caacatattc accatcttct gaaccgagtg catgtctctc aatttttaat
     2041 cctttttctt cataatattt ttttgtttct atatataatg attctcgctt ttgtatgtag
     2101 tctttcactt cattatttgg aaagttatat gaatccacta atgtttcgcc atttataaaa
     2161 gttttctcgg tagcaacttt ttcatcccaa taatggattt cttcccaagt aaaaaatatc
     2221 tcagttccat ctggaatgaa tgaaaacctg gtaataatat ataaataatt attgaaattt
     2281 acttcataat attcaggaat atcttcaaat tccatatagt aatttgtata attaaaatta
     2341 ctattatctt tttttacaaa gggtattttt tcaataaatt tatatattga ttgttcattt
     2401 ttaaaaattc ctatatcata gcttctttca aattg
//
LOCUS       DAELXX010000225         2430 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.228, whole genome shotgun sequence.
ACCESSION   DAELXX010000225 DAELXX010000000
VERSION     DAELXX010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.228"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            361..1887
                     /locus_tag="KPH29_003672"
     CDS             361..1887
                     /locus_tag="KPH29_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG1469229.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKNIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NNGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            1915..>2430
                     /locus_tag="KPH29_003673"
     CDS             1915..>2430
                     /locus_tag="KPH29_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1469230.1"
                     /translation="MNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGLED
                     TMIDMVDALIGT"
BASE COUNT          955 a          208 c          441 g          826 t
ORIGIN      
        1 taaaataaat ttttaagtaa atatttttat agagagattg ttatattaca tatcagaaaa
       61 atattaaacg aactaaatca ataaaaaagt tggtggctaa caatttataa ttgttagcac
      121 aatataatct tatgtattga aatctagaat atcctcattt ttacttagca taaatactat
      181 tgattaagaa aataatatag aaattataaa tatatataag tttagtctgg tgtataaaac
      241 aagaaaacta agtgattttt catttagctt tttattaagc ttatttttta tactatttat
      301 aataaataaa ctccatgatg ggaaaactat tactatttaa tatgtaaagg agtattttaa
      361 atgaatgaat catattggtt tttaaactca agtccaaaag aatataacaa attaggtgaa
      421 aatataaaaa cagattgtct tatagtaggt ggtggaataa ctggattgac aactgcatat
      481 ttattagcaa aagaaggtaa aaaagtagtg cttgttgagg ctgataaaat aggatatgga
      541 acttctggaa ggaatactgg taaggtaact tgtcagcatg atatatttta ctctaagata
      601 gagaaaaaat atggattaga taaagcaaaa tcatattata atgcaaataa tgaagcttta
      661 aatttagttg aacaaataat agaagaaaac aatataaaat gtgattttaa aagggaaact
      721 tcttttgtat ttacagaaaa agaggatact ataaagaata ttaaagatga atatcgtaca
      781 tgtaaaaaaa taggaataaa ttgtgagtat catgaaacta tagaaaatat tccattagat
      841 ataaaggggg ctattagttt tactaatcaa ggtcaattta acccaaagaa gtatattgat
      901 ggattggcaa aagcagcagt aaatttaggg ctaaaaatat atgaaaatac accagtagta
      961 gatcttgaaa aaggaaaaat atgtagggtt aaaacaagag aagataatat aatagaagct
     1021 gagaatgtta taatttcatc tcatagtcca tggtatgatg gattgaattt ttattttgca
     1081 aaagaatatg cagagcgtgc ttatttaatg gttgcagttt tggaaaataa attagcagat
     1141 ggtatgttta taagtataga tgacccatca ataactttta gacaatataa taatggaagt
     1201 gaaaatttac tgatttttgg aggaggagac cataaagttg gccagggagg aactgagaaa
     1261 gaaatatttg atgatttaga gcattatggt aaagaagtct ttaaggtaaa agattttaag
     1321 gggaaatggt ctgctcaaga taatatgagt tttgacaatg ttccttatat tggttatatc
     1381 aacaaaagag aagacaatat ttatgtagct acaggattct ctaaatgggg aattactaat
     1441 ggaactgctg ctggaataat aataaaagat ttgataataa ataataattc tgattataaa
     1501 gatactttta atccatccag attaggtagc tacttttcaa aagattttat aaaggaaaat
     1561 gcaaacgtag caataaatta tgtaagtgga aaattaaaaa taggtagtgg agatatgcct
     1621 aaaaataatg gagaaggaaa gattgttaat attgatggaa aaagatatgg tgtatataag
     1681 gatgataatg gagattttta tattgtagac acgacatgta ctcatttagg atgtgaactt
     1741 aattttaaca gtgaagaaaa aacatgggac tgtccatgtc atggttctag atttgactat
     1801 aagggaaata tacttgaagg accagctctt aagccattaa aactttatgg acatggtgat
     1861 aatgatgtta atcctaagtt attataaaat tatttttagg aaggtaaagt gtatatgaat
     1921 aaacttttaa agccaataac tatactattg tatatagttt atattttatc tgccgtattt
     1981 tttgtagcaa acaaagaata tggaagtcta ggtctagtag caattagttt agttggtact
     2041 ttcattttga gttttataaa taagcaaact gaaagattat tgagtaaaga attatatata
     2101 atgcttgctt tatttataat gttttcatca ttgttaggta cttgttttaa tttttatgat
     2161 ataaattatt atgatgattt tttgcattta tggtcaggta taatatcttg tattgtggct
     2221 ttttctataa ttaaatactt ttatacacaa agagagataa gtaaaatgag taaagtgttt
     2281 ttagttatat ttttatttat gtttagtatg ggagtagcga gtttatggga aataggtgaa
     2341 tttttaatgg atacttttgt tgggactact acacaggccg gggggttaga agatactatg
     2401 attgatatgg tagatgcttt aataggcaca
//
LOCUS       DAELXX010000226         2375 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.229, whole genome shotgun sequence.
ACCESSION   DAELXX010000226 DAELXX010000000
VERSION     DAELXX010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.229"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            940..2295
                     /locus_tag="KPH29_003674"
     CDS             940..2295
                     /locus_tag="KPH29_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBG1469231.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKVEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
BASE COUNT          841 a          257 c          399 g          878 t
ORIGIN      
        1 tactgcatac aaaaatatat ttgagcaaat aagtaaagta ataaaaaaat caagatttca
       61 tatctaaaaa ctattctgaa cattaattta atttatattt tcataggaaa tattttatat
      121 aatacactta acaaatataa tttaaatttt tctaattatt tactgtcgat taatagattt
      181 ttttcttgca tataagtgca tatttatggt acgatttaca tggtaaaata aaaaagggga
      241 ggtgaaaaat aaatattcgt gaaaaagttt tcagtttgag gtattttatt aataatatat
      301 tttgaggtat tttataaata atatattata aataaagagt ataacctcaa taaatagtat
      361 caaagcgaat atatgggtaa ttactttaaa ataacagttt ttatgtttag attcacagtg
      421 tttattataa actaatgtgt aagaaatagg tctaatctgt gaatagatag tagtttattt
      481 atttaagttg agaattggtt gagtgaagta atatataaat ttaaaaagta aataaaaaca
      541 ttatttgaac aaaatatatg ttgctttatt cgagactcct ttaaaaaagc actaaaatgt
      601 aattacagaa gttgctacaa aagggggatt ttagtttgta aaatactatc acgtaaaaaa
      661 taagaattgc atatggcgaa ggttttttca tggtattaga tgagaatata ttaatttata
      721 actatatctg aaaatagtga atttatataa tttaaattta tgattaagaa gtgtattaat
      781 tttattccat gagaatatgt actctacaaa taaatgcgca attatcttaa taataattac
      841 aaattgtttt acaaaataat aatattatat ctttcataaa agaaagaatt tggacaaaac
      901 gtaaaaaata aaaatacaat taagaaagga tttaaaatta tgagctcaac agtaaaaaag
      961 aagggtaaat ttcctatggg attttatatc tgctcaacaa ctttctcatt tgaaagagca
     1021 gcatattatg cttcaaagta cttaatatat atattcctta caacagctat agttcatggt
     1081 ggtcttggaa tagataaagg acaagctgct ataatgcaag ccaaccttgt tgcattcaca
     1141 taccttgcac ctataattgg gggatatata tcagatagat ggataggagc tagatatact
     1201 atacctatag gtatgcttat catgggggta ggttactatc ttggttctat tgcaactaca
     1261 gtttctatgg taaatgcaat gataatctta gtttcgattg gtactgcatt ctttaaaggt
     1321 aacgtatctg cggtaaatgg acagcttttt gatagtcaag aagaacttga tacagcattt
     1381 tcagtacaat attcttttgt taatataggt tcttttattg gtactatagc agttggtatt
     1441 ctttacctta aaacatttgc aaaaaatggt gtacttggat tttctcaatg tttctttata
     1501 gcagctgtac tttgtgtaat aggtgcaata tggttcatat atggatggag attccttgga
     1561 aatgcaggaa agagaccttt taaagaaggt gtagttgctg aaaaagttga agaaaaagat
     1621 aagtctcctc ttacaagcat ggataagaag agaatttggg ctattatact tatatcattc
     1681 ttctctgtaa ttttctgggt attctggtat ctaacttatc ttgcagttta tgattatggt
     1741 gcagcatttg taaacatgaa tgttggagga tttgatgttc ctcttgcttg gtttgactca
     1801 ttaaattcac ttgtatgtat agtattagga ccagttcttg gagcattatg gtttaaactt
     1861 gctagtagac cacaaggaga tatgagttta tttaaaaaga caggtcttgg attaatattc
     1921 ttaggtcttg cattcttaat gcttgttggt gcagaatttt caagaggagt tggagctcct
     1981 gaaactgcta aagctagtat actatggata atcatgtttg gtattctttt aagtcttggt
     2041 gaaatgttat tctcaccact tggtaactct tttgtaagta agtatgcacc taagaagctt
     2101 ctaggtgttc ttatgggagt atggacattt gctactttta tagctggtaa aggatatgga
     2161 tatatatatg catttactct taaatttgat atgattaaag tatatactat aattccagta
     2221 atactatttg ttgcagcgat attattattc ttatctgata agacattaag tagacttgtt
     2281 gaggatgata aataatcagt aagtaattat tatgttttat agattaatct actgtaatta
     2341 tgttgtatta gattgattta taactaatta ataat
//
LOCUS       DAELXX010000227         2293 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.230, whole genome shotgun sequence.
ACCESSION   DAELXX010000227 DAELXX010000000
VERSION     DAELXX010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.230"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            142..2154
                     /locus_tag="KPH29_003675"
     CDS             142..2154
                     /locus_tag="KPH29_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG1469232.1"
                     /translation="MKKSVALVNDSRKDLIDFLENNLKLVFGDSININRYFINEINDN
                     DIINDDVILVMSVERLDKIINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNALIVND
                     SDETTLETISLFYKIGVTNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDIFDLGH
                     RYIDISTFIEIINLLQIDSKEIQSNLVKYSEEIISLDTGIKDKYKELFLKIEELDTIL
                     NLSKDGILFTSKDGEINTYNSKVKDILDINEDIYGKYIDDIFVDSLKVLLSEKEILDK
                     VVVFNKKYINVNKKNIYNRDEKMGTYYSLQEITYIKKLEQNLTKKLREKGQIAKYTFK
                     DIKTNSPKMFECIDLAKKVSKSDLSILIRGESGTGKELIAQSIHNNSNRKNQPFIAVN
                     CAAVPENLLESQLFGYDKGTFTGGLKDGKQGLFELANNGTIFLDEIGDMPLELQTKLL
                     RVLQEKQIMPVGSHNVINIDVRIISATNKNLEQMIDNSQFREDLYYRLNTIPINIPPL
                     RERKEDILIIMEDLINKKLVITPEAKKLIQNYMWKGNIRELQNVTSYLNIMCEDIVLE
                     KDLPPNLRSSDNKNTSLKLKYSKNDILNILEILILNKESDVGIGRGLILKALLDKNLQ
                     ITEGKIKKIFEYLKKEELIICSSGRYGSKITQKGEDFYNKLKYKGL"
BASE COUNT         1022 a          222 c          329 g          720 t
ORIGIN      
        1 aagaaagaaa gtgtcttaat gcaactttta taaagtcatt atagacactt tcttttttat
       61 ttgataaatt taaattatca aaattacatt gctttataca actattttgt tggaaactat
      121 ttattatata aggagaaata aatgaaaaaa tcagtagctc tggtaaatga tagtagaaaa
      181 gatttaatag actttttaga aaataatttg aaattagttt ttggagattc tataaatatt
      241 aatagatact tcataaatga aataaatgat aatgatatta taaatgatga tgtgatatta
      301 gtaatgtcag tagaaagatt agacaaaatt ataaataata tactagataa gaaaaaagtg
      361 attgtggtaa gaagaacttt tagggaagat aaaatttata atctactatc tttaccacag
      421 ggtacaaatg ctctaatcgt aaatgattct gatgaaacta ctctagaaac tataagttta
      481 ttttataaaa ttggagtgac taatattaga ccaattccat atatgaatga taataactat
      541 aaaaatataa aaatagctat aactccagga gttccagaaa aagtacctag ctttatctca
      601 gatatatttg atttaggtca cagatatata gatatttcta catttataga aataataaat
      661 ttattacaaa ttgattcaaa ggaaatacaa tctaatctag ttaaatactc agaagaaata
      721 ataagtttag atactggtat aaaagataaa tataaggaat tatttttaaa aatagaagaa
      781 ttagatacaa tattgaattt atcaaaagat ggtatattat ttacttcaaa agatggtgaa
      841 ataaatacat ataatagtaa agtaaaagat attttagaca taaatgagga tatatatgga
      901 aaatacattg atgatatatt tgtagatagt ttaaaagttt tattgagtga gaaagagata
      961 ctagataaag ttgtcgtatt caataagaaa tatataaatg taaataaaaa aaacatatac
     1021 aatagggatg aaaaaatggg cacatattat agtttacaag aaataacata cataaaaaaa
     1081 ttagaacaaa atctcactaa aaaattaaga gaaaaaggac aaatagcaaa atatacattt
     1141 aaggatataa aaacaaatag tcctaaaatg tttgaatgta tagatttagc taagaaggtt
     1201 tctaaatctg acttaagcat acttatcaga ggtgaaagtg gaactggtaa agaactcata
     1261 gctcagtcta tacataacaa ctcaaataga aaaaatcaac cttttatagc agtaaattgt
     1321 gcagccgttc ctgaaaattt attagaaagc cagctattcg gatatgacaa gggaactttt
     1381 actggaggtc taaaagatgg aaaacaagga ttatttgaac ttgctaataa tggaacaata
     1441 ttcttagatg aaataggcga tatgccttta gaattacaaa caaaactact tagagttctt
     1501 caagagaagc agataatgcc tgttgggtct cacaatgtaa ttaatataga tgttagaatc
     1561 atatctgcta caaataagaa tttagaacaa atgattgata atagccaatt tagagaggat
     1621 ttatattatc gattaaatac aattccaata aatatacctc ctctaagaga aagaaaagaa
     1681 gacatattaa ttattatgga agatttaatt aataagaaat tagtaatcac accagaagca
     1741 aaaaaactta tacaaaacta tatgtggaaa ggtaatataa gagaactcca aaatgttaca
     1801 tcctatttaa acattatgtg tgaagatatt gtattagaga aagatttacc tccaaactta
     1861 aggtcttcag ataacaaaaa tacatcatta aaactaaaat atagtaagaa tgatatattg
     1921 aatatattag agatactaat tttaaataag gaatcagatg ttggtattgg taggggatta
     1981 attttaaaag cactactcga taagaacctt caaattacag aaggaaaaat taaaaaaata
     2041 tttgaatatc taaaaaaaga agaacttata atttgtagtt caggaagata tggaagtaaa
     2101 ataactcaaa aaggagaaga tttttacaat aaattaaaat ataaaggtct ataaaattat
     2161 aatttatcaa acaaagaaat ctattcagga ttaaagtact ccttcaatag gtttttttat
     2221 ttttcaagta ccctaaaatt ataatttatt ttgtacataa ataataggaa ttaataggaa
     2281 taggataatt ttt
//
LOCUS       DAELXX010000228         2163 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.231, whole genome shotgun sequence.
ACCESSION   DAELXX010000228 DAELXX010000000
VERSION     DAELXX010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.231"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(94..444)
                     /locus_tag="KPH29_003676"
     CDS             complement(94..444)
                     /locus_tag="KPH29_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1469233.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKTVTSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
     gene            complement(667..858)
                     /locus_tag="KPH29_003677"
     CDS             complement(667..858)
                     /locus_tag="KPH29_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1469234.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            complement(977..1525)
                     /locus_tag="KPH29_003678"
     CDS             complement(977..1525)
                     /locus_tag="KPH29_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG1469235.1"
                     /translation="MGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDSLKKADIEPVL
                     LKDNMNIVINSAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGDAEEERLSEFI
                     NGNTLKYDFVKMPHHGRYDKLTETFLESTSPQYAVITCSEKKEPEEGVLKILERLNIK
                     TFLTSSGEVVINSDGKTLSVNQ"
     gene            complement(1668..>2163)
                     /locus_tag="KPH29_003679"
     CDS             complement(1668..>2163)
                     /locus_tag="KPH29_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG1469236.1"
                     /translation="SLNKLAKLQRELSRKTIGSLNRNKARLKVAGLQEHIANQRNDFL
                     QKLSTKLIKENDIICIEDLQVKNMIKNRKLSRLISDVSWSEFVRQLEYKANWHGRQIV
                     KVGKFFASSQICNKCGYKNEEVKNLNIREWICPSCNETHDRDINASINILKEGLRLIT
                     IQNK"
BASE COUNT          687 a          383 c          215 g          878 t
ORIGIN      
        1 atataactaa taaaataaga atattatatt tttatatata agtataatat tctcacctta
       61 acattaattt atttcaatat tttcatatat cttttattct aaattcaata cttttctaat
      121 atcttctata ccttgataaa cttcttgttt agaaaaattc atagcttcta attcttcatc
      181 agtcttttcc ataagcatag tataaaattc tattgctata tactgcatat tttcactagt
      241 tacagtctta gctttatcaa ataaaatttc ttctgcttcg tttacatttc cctgaaaaac
      301 taatcttttt aaagttataa gtataacttc ttcttctgta ttatacacta aatctttttc
      361 ttcatattca ctgtattttt ttcccaataa aagtcttgca accataagtt ttatctcatt
      421 gaaaatctta tccactatag ccatttttac atctcctata aataaatttt attttctttt
      481 taattatatc agactttatt gtaaaaatta ttatatgtaa atgaaataat tcaaaaataa
      541 attctaacaa taaaaaaaag aaggtagcgg gggatttccg actaccttca aacactagat
      601 tgtgattggt ttggtatttt atttataccc accataagct ttattaaatc atctaaattg
      661 atttttttag catccacatc ctccatcaaa aacgtcacca cattcacaaa gtattaataa
      721 taatattaag aaaggtatcc atgcacataa gtccatttca gcccaacaat cttggaatgc
      781 taagaataag aagaataata ttattatcca ccaagcacat ccaccaaatc cgccttcacc
      841 aaaaaatcta tccaacataa tatgttacct cctagtcatt ttgtatgtgg agtttcagct
      901 cctaaaatat actatgttta aggggtaata tttgctaatg atttttttaa atttttttta
      961 gatattttaa aaaaaattat tgattcacac tcaatgtctt accatcacta ttgataacta
     1021 cctctccact agaagtcaaa aatgttttta tattcaatct ttccaatatt tttaaaacac
     1081 cttcctctgg ttcttttttc tctgaacatg taataacagc atattggggt gaagtagact
     1141 ctaaaaatgt ttcagttaat ttgtcatatc ttccatgatg aggcatttta acaaaatcat
     1201 attttaaagt atttccattt ataaactctg acaatctttc ttcctctgca tctcctgcaa
     1261 ataaaaactt atgtgctcca tagcttatat tagtgattat tgaataatca tttgaactct
     1321 cgtacttgct ttttgaagcc ggatgtatgt ttatttctgc actattgatt acaatattca
     1381 tattatcttt tagtagcact ggttctatat cagctttttt aagtgagtca atatattcat
     1441 catattgctt actatctttc ttataatttg cttgtataat attctttact ttaacactag
     1501 ataacacact atctactcca cctatatggt ccttatctaa atgggttaaa ctgaacctcc
     1561 tatgactaaa gtcacagggt tcctgcttca tagaattttg catactaaca tatgtcttac
     1621 taattctcca caggctatcc ccgtagttcc tacggttctt acatacgtta tttattttga
     1681 attgttatta gtcttaatcc ttcttttaat atatttatac ttgcatttat atctctatcg
     1741 tgagtttcat tacaactagg acaaatccat tctcttatat ttaagttctt aacttcctca
     1801 tttttatacc cacatttatt gcatatttgt gaacttgcaa agaatttgcc tacttttaca
     1861 atttgtctac catgccaatt agccttgtat tctaattgac gaacaaattc tgaccatgac
     1921 acatcagaaa ttaagcgaga tagtttacga tttttaatca tattctttac ttgtaaatct
     1981 tctatgcaaa taatatcatt ctcttttatt aatttagtag atagtttttg tagaaaatca
     2041 tttctttgat tggctatatg ttcttgaagt cctgcaactt ttaatcttgc tttattccta
     2101 tttaaactac cgattgtctt tctcgataat tctctttgta atttagcaag tttgtttaat
     2161 gac
//
LOCUS       DAELXX010000229         2159 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.232, whole genome shotgun sequence.
ACCESSION   DAELXX010000229 DAELXX010000000
VERSION     DAELXX010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.232"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            83..>2159
                     /locus_tag="KPH29_003680"
     rRNA            83..>2159
                     /locus_tag="KPH29_003680"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          645 a          428 c          610 g          476 t
ORIGIN      
        1 atctaatttg taatatataa agctgataac tttttaaaat tatcgaagtt gatagcttct
       61 aatctatcaa acctttttaa ctggtcaagt tattaagggt gcagggcgga tgccttggca
      121 ctaggagccg atgaaggacg tgataagctg cgataagctt cggggagttg cacgtaaact
      181 ttgatccgaa gatttccgaa tgaggaaact cacttagagt aatgtctaag tatcattaag
      241 tgaatacata gcttaatgag gggaactcag ggaactgaaa catctaagta cctgaaggaa
      301 gagaaagaaa ttcgattccg taagtagcgg cgagcgaacg cggattagcc caaaccaata
      361 aagttttctt tattggggtt gcggacatat cataacgaag aggtatcgta attgaagagg
      421 tttggaaaga cccaccacag aaggtaatag tcctgtatgt taaacgagaa gacttcagat
      481 atgatccaga gtaccacggg acacgtgaaa ccctgtggga agcaggaggg accaccctcc
      541 aaggctaaat actacctagt gaccgatagc gtatagtacc gtgagggaaa ggtgaaaaga
      601 accccgggag gggagtgaaa tagaacctga aaccctgcac ttacaagctg tggaagcaca
      661 tttcttgtgt gaccgcgtac tttttgtaga acgggccaac gagttacgtt aagtagcaag
      721 gttaagcact taaggtgcgg agccgtagcg aaagcgagtt ttaactgagc gttcagttac
      781 ttgacgtaga cccgaaaccg ggcgacctac ccatgagcag gatgaagcga aagtaaaatt
      841 tcgtggaggt ccgaacccac gagcgttgaa aagctcgggg atgacttgtg ggtagcggtg
      901 aaattccaat cgagcccgga gatagctggt tctccccgaa atagctttag ggctagcctc
      961 aaggtgagag atacggaggt agagcactga atgtcctagg gggtattgca cctaccgaag
     1021 actatcaaac tccgaatgcc gtcatcttat acttgggagt cagactgtgg gtgataagat
     1081 tcatagtcga aagggcaaca gcccagatcg tcagctaagg tccctaaatg taagttaagt
     1141 ggtaaaggat gtgggattgc acagacaacc aggatgttgg cttagaagca gccactcatt
     1201 caaagagtgc gtaatagctc actggtcgag tgatcctgcg ccgaagattt ccggggctaa
     1261 aacttactac cgaagctacg gcatcagtaa tgatgggtag gggagcttcc catacgggtt
     1321 gaagcatgac cgtaaggaca tgtggacagt atgggagtga gaatgttggc atgagtagcg
     1381 agatgtgggt gagaatccca caggccgtaa acccaaggtt tccaggggaa ggttcgtccg
     1441 ccctgggtta gtcgggacct aagctgaggc cgaaaggcgt aggtgatgga caacaggttg
     1501 atattcctgt actaccgata accgtttgag agaagggatg acacagtagg ataagctaag
     1561 cacactgttg gttatgtgtg cccaagcatt gaggcagtca aagtaggcaa atccgctttg
     1621 ataatgctgg gatgtgatgg ggagcgaaat ttagtagcga agtagctgat ttcacactgt
     1681 caagaaaagt ctctatcgag gttaaaggta cccgtaccgc aaaccgacac aggtgggtga
     1741 ggagagtatc ctaaggccag cgagagaact gttgttaagg aactcggcaa aatgaccccg
     1801 taacttaggg ataaggggtg ccaccatcag gtggccgcag agaataggcc caagcgactg
     1861 tttaccaaaa acataggttt ctgctaagtc gcaagacgat gtataggagc tgacgcctgc
     1921 ccggtgctgg aaggttaagg ggatctgtta gagcaatcga agcagtgaac ttaagcccca
     1981 gtaaacggcg gccgtaacta taacggtcct aaggtagcga aattccttgt cgggtaagtt
     2041 ccgacccgca cgaaaggcgt aacgatttgg gcactgtctc aacaacagac tcggtgaaat
     2101 tgtaattccg gtgaagatgc cggatacctg cgacaggacg gaaagacccc atggagctt
//
LOCUS       DAELXX010000230         2050 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.233, whole genome shotgun sequence.
ACCESSION   DAELXX010000230 DAELXX010000000
VERSION     DAELXX010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.233"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            317..763
                     /locus_tag="KPH29_003681"
     CDS             317..763
                     /locus_tag="KPH29_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBG1469237.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
     gene            784..1827
                     /locus_tag="KPH29_003682"
     CDS             784..1827
                     /locus_tag="KPH29_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBG1469238.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
BASE COUNT          839 a          217 c          383 g          611 t
ORIGIN      
        1 ggattaagac taataacgat tcaaaataaa taacttatgt aagaaccgta ggaactacgg
       61 ggatagcctg tggagaatta gtaagacata tgttagtatg caaaattcta tgaagcagga
      121 accctgtgac tttagtcatg ggaggttcag actactttgt aacaatttta acaaagtatg
      181 ttgatataat actattaaat taatcttaga ttagtttatt ctattgattt gtagtttaaa
      241 tttatattca aaaacattgt ataaacatat aatttatata gggttttata attcatatga
      301 aaagagggta gatagtatga agttaatatg gttgatagta gcagtcttat ttggaatagc
      361 agaaatgtta acaccaagct taacattgat atggtttagt gtaggagctg taatacttat
      421 atttttaagt agctttatag agagtatttt cttacaaatt ttaatatttg cagtaatttc
      481 tatagcaatg ttagtcgtag ctactaaaaa aatagtaaaa aaagataaag gctataaatc
      541 taacactaat ttacaagcaa tgatgtctaa aaaaggtatt gttacagaag agatttctcc
      601 aaataacaca ggtctagttg tagttgaaca tgaaacatgg acggctattt ctatagatgg
      661 tgaaaagata gaaaaaggtt ctacagtaga agtattaaaa attgaagggg taaaattagt
      721 tgttaaaaaa gtagacgcca cagttaatgt gacaaaccaa tagaataaag gaggatttat
      781 agtatgggta ctaaaattgt tttaagtatt gtattaatag tagtagtcgt agcaataagt
      841 ttaacgtgta taagagttat aaaacaatct aaagtaggta taataatgag acttggtaag
      901 tttcaaaaag tggctgaaac aggtgtacat ttcttaatac catttttaga taaaatggca
      961 tatgtaattg accttagaga aattgtaata gattttccac ctcaaccagt tataactaaa
     1021 gataatgtaa ctatgcagat tgatacagtt gtatattata aagttacaga tccagttaga
     1081 tatgtgtttg agatagctaa tccaatagct gctattgaaa atttaacagc tacaacacta
     1141 agaaatataa ttggtgaact tgatttagat gaaacattaa catcaagaga tataataaat
     1201 gtaaaaatga gaactatcct tgatgaagca acagataaat ggggtataaa agtaaataga
     1261 gtagagttaa aaaatataat gcctcctcag gatattcaag ttgcgatgga aaaacaaatg
     1321 agagcagaaa gagaaagaag agaggcaata cttcaagcag aaggtaataa gtcagctgca
     1381 atacttcaag cagaaggtga aaaacaatct gcgatattaa cagcagaagc caaaaaagaa
     1441 gctatggtac gtgtagcaga aggtgaaaaa gaatctgcta tattagtggc agaaggtgaa
     1501 gctgaagcta taagacagac cgctattgct aaggcacaag gtgaggctga aatgataaaa
     1561 agaactcaaa tggcaacagc agaaggttta aaattagttt tttcagcaat gaaagaggct
     1621 gatatagaca ataatattct agcattaaaa tctatggaag ctcttgaaaa aatggctgaa
     1681 ggtaagtcaa caaaactggt tttaccttca gaagcagtta atttcctagg aacattcaaa
     1741 ggaataaagg aagttatgag tgatgataac aaagaagtac ttgatataaa agaagtttta
     1801 aatgataatg aatcattaaa aaaataaaag agttttgtta tcataggaag gatatggaaa
     1861 ttaaaggtcg aattaattaa atatagcaca aaaatgcacg ttgaaaagaa gaggacttat
     1921 aaatttttaa gtcttcttct ttgtttttat tagaaagatg aaattataaa aatgaattaa
     1981 cagaatcttt atacaattta tcctttacta atgtaaaaag tgttatataa tattatataa
     2041 aacatatagc
//
LOCUS       DAELXX010000231         2048 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.234, whole genome shotgun sequence.
ACCESSION   DAELXX010000231 DAELXX010000000
VERSION     DAELXX010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.234"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(314..1642)
                     /locus_tag="KPH29_003683"
     CDS             complement(314..1642)
                     /locus_tag="KPH29_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG1469239.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGDKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIVIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGIKR"
BASE COUNT          739 a          351 c          225 g          733 t
ORIGIN      
        1 ttttatataa cagaaagatt taattttcat aaaacctatg tataatattt gttatataga
       61 tatcacttag tttgaaaaaa aaacacttta aaataaagta tcttaaagca aataattttt
      121 tagatgttta attttataat tcaaaaacta aaaataaagt gaatacctta aaataaactt
      181 tttagttcat agaacactct ttttttataa aataaaacat ctctttaccc ttttaacaat
      241 aaaaaagaga cttatctcta ttttgagaca gtctctttta ttttaattat aaagcatttg
      301 taaactactt ggtttatctt tttattcctc tagtcatata agcatacact ggtaagccta
      361 tagctgttat tacaagacct cctatagata tcatagtagt tttcatccct gcaaaacaca
      421 attgattaac tacaacaaat ataccacttg ctatagctat tataggaaca ataggatata
      481 atggaacttt atactctctt ggtatatttg gatgagtctt tctaagtttc ataaccccta
      541 taaaagttaa tacatagaat atccaagtag caaatacagc taagtcagtt aataaattaa
      601 attgacctga caaagcatat atagatgcaa taacagatac taatgctatt gcatttgctg
      661 gaacttgagc agaatttaat ttagaaaata taccatactt tggtaaagtc ttatctgtag
      721 ctaaagtata tgccaccctt gaaccagtta gtaaaaatcc atttaatgct ccaaacactg
      781 aaattaatat acctacagat attatttttc cacccattga accaaatata acttcagcaa
      841 ctgctgatgc aggagaagca taatttgcta attcattagc tggtaatacc caaagatacg
      901 ctaaatttat tataaaataa acagccataa caactgacaa tccacctatt atagcttttg
      961 gaaggtcttt tccaggattc ttcatttctc cagctaaagt accaacattc atccatccat
     1021 caaaagcaaa taatatagca attaaaactt gacctagtac actacccagg cttaatcctt
     1081 caccaaccat aggtgtaagt attggattat ttccaccacc tttaataaat ccaacaacca
     1141 ttattaaaat aagtggtatt aacttacaaa ttgtagagac agtttgtatg actcctccag
     1201 tttttgaacc taacatatta agccctgcaa ctattactat aattccaatt gctattggaa
     1261 gtaaaagtga tgggctacca attagtgctg atgcttgttc tccaaatata acacctaatg
     1321 ctgccatcat ccctggataa aataatacaa tttgcatcca tccagttaaa aaacctaatt
     1381 tgtctccata aatttctttt atatatacca tcattccacc agtttttggt atcgcaactg
     1441 atacttctgc agctgtaaga ccagctgtta tagttatgat tccagctatt aaccatgcca
     1501 atataccaag acctggagct cctccagtta atgtatagac tgcttgtggt ttaaaaaata
     1561 cgcccgctcc tataacagaa ccaacaacag ttgataatgc tgctgatacg ccaagagtct
     1621 ttttcagttc attacttccc attcttttcc tcctcattaa tcctatttaa ttttaatttc
     1681 atagttaaat aagcatatat tatgccaata tatagaaaaa tagctttttt atttattaaa
     1741 ataaaataaa ctctacttag ctttcgtaat cttttacttt tagttatgtt taaatataaa
     1801 aaaattataa aaaccaaaaa tccaaatata ataatattta taaataaacg tattttttat
     1861 attgtataat atacattgta tatttgtaac actaaaaata gattataact attaagttaa
     1921 attgattttg atttttttgc aataaaattt catttttttc aaaaacattt tggcttcttt
     1981 ctgtaatttt ctatctttaa ggtcttttat ataatagtat gatttttgct tgttttatag
     2041 tctatttt
//
LOCUS       DAELXX010000232         1988 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.235, whole genome shotgun sequence.
ACCESSION   DAELXX010000232 DAELXX010000000
VERSION     DAELXX010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.235"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            178..294
                     /gene="rrf"
                     /locus_tag="KPH29_003684"
     rRNA            178..294
                     /gene="rrf"
                     /locus_tag="KPH29_003684"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            301..375
                     /locus_tag="KPH29_003685"
     tRNA            301..375
                     /locus_tag="KPH29_003685"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:333..335,aa:Asn,seq:gtt)
     gene            380..454
                     /locus_tag="KPH29_003686"
     tRNA            380..454
                     /locus_tag="KPH29_003686"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:413..415,aa:Glu,seq:ttc)
     gene            460..535
                     /locus_tag="KPH29_003687"
     tRNA            460..535
                     /locus_tag="KPH29_003687"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:493..495,aa:Val,seq:tac)
     gene            541..617
                     /locus_tag="KPH29_003688"
     tRNA            541..617
                     /locus_tag="KPH29_003688"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:575..577,aa:Asp,seq:gtc)
     gene            628..702
                     /locus_tag="KPH29_003689"
     tRNA            628..702
                     /locus_tag="KPH29_003689"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:660..662,aa:Thr,seq:tgt)
     gene            717..801
                     /locus_tag="KPH29_003690"
     tRNA            717..801
                     /locus_tag="KPH29_003690"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:751..753,aa:Tyr,seq:gta)
     gene            811..884
                     /locus_tag="KPH29_003691"
     tRNA            811..884
                     /locus_tag="KPH29_003691"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:843..845,aa:Gly,seq:tcc)
     gene            895..971
                     /locus_tag="KPH29_003692"
     tRNA            895..971
                     /locus_tag="KPH29_003692"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:929..931,aa:Arg,seq:tct)
     gene            981..1056
                     /locus_tag="KPH29_003693"
     tRNA            981..1056
                     /locus_tag="KPH29_003693"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1014..1016,aa:Gln,seq:ttg)
     gene            1068..1143
                     /locus_tag="KPH29_003694"
     tRNA            1068..1143
                     /locus_tag="KPH29_003694"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1101..1103,aa:Lys,seq:ttt)
     gene            1146..1234
                     /locus_tag="KPH29_003695"
     tRNA            1146..1234
                     /locus_tag="KPH29_003695"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1180..1182,aa:Ser,seq:tga)
     gene            1252..1342
                     /locus_tag="KPH29_003696"
     tRNA            1252..1342
                     /locus_tag="KPH29_003696"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1286..1288,aa:Ser,seq:gct)
     gene            1351..1427
                     /locus_tag="KPH29_003697"
     tRNA            1351..1427
                     /locus_tag="KPH29_003697"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1385..1387,aa:Pro,seq:tgg)
     gene            1513..1588
                     /locus_tag="KPH29_003698"
     tRNA            1513..1588
                     /locus_tag="KPH29_003698"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1546..1548,aa:Trp,seq:cca)
     gene            1649..1725
                     /locus_tag="KPH29_003699"
     tRNA            1649..1725
                     /locus_tag="KPH29_003699"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1683..1685,aa:Pro,seq:tgg)
     gene            1732..1808
                     /locus_tag="KPH29_003700"
     tRNA            1732..1808
                     /locus_tag="KPH29_003700"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1766..1768,aa:Ile,seq:gat)
     gene            1812..1887
                     /locus_tag="KPH29_003701"
     tRNA            1812..1887
                     /locus_tag="KPH29_003701"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1845..1847,aa:Phe,seq:gaa)
     gene            1895..1971
                     /locus_tag="KPH29_003702"
     tRNA            1895..1971
                     /locus_tag="KPH29_003702"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1929..1931,aa:Met,seq:cat)
BASE COUNT          505 a          449 c          535 g          499 t
ORIGIN      
        1 aatgtattta gcttatcgat actaataggt cgaggacttg accaatatta tttgatgtta
       61 ttcattaaga tatatgtgca gtttttagag tgttaactct aaaaaaaaat aaaaaaagta
      121 ttgacaaaaa tagtaaaaaa tgttaatatt atacttgtcc tagcaacaag aaaaaaatgt
      181 ggttataata gcagagaaga tacacctgtt tccattccga acacagaagt taagttctct
      241 agcgctgatg gtacttggtg ggaaactgcc tgggagagta gggcatagcc acgtgaatgt
      301 tccaaggtag ctcaacggtg gagcaaccgg ctgttaaccg gtaggctgtg ggttcgagcc
      361 ccacccttgg agccattatg gctcgttggt caagaggtta agacaccgcc ctttcacggc
      421 ggtaacaggg gttcaattcc cctacgagtc accatttgtg ggatcatagc tcagctggga
      481 gagcacctgc cttacaagca gggggtcaca ggttcgagcc ctgttggtcc caccatttgc
      541 ggcctggtag ttcagttggt tagaatgcca gcctgtcacg ctggaggtcg agggttcgag
      601 ccccttccag gtcgccaaaa taaaaatgct ggtgtggctc aacggtagag cagctgactt
      661 gtaatcagca ggttgtaggt tcgattccta tcaccagctc catgtaaaac taatacggag
      721 gatttcccga gcggccaaag ggggcagact gtaaatctgt tgtcatcgac ttcggtggtt
      781 cgaatccacc atcctccacc aattaaatat gcgggtgtag ctcaatggta gagttctggc
      841 cttccaagcc agctgtgagg gttcgatccc cttcacccgc tccaaagaaa gcgtgggtgc
      901 atagctcagc tggatagagc aacgcccttc taaggcgtgt gtccggggtt cgaatccctg
      961 tgcgctcacc acatatatat tggggattag ccaagtcggt aaggcacatg actttgactc
     1021 atgtatgcgt aggttcgagt cctgcatccc cagccaaata aaaatatggt ctattagctc
     1081 agtcggtaga gcacctgact tttaatcagg gtgtcccgcg ttcgagtcgc ggatagatca
     1141 ccaatggaga ggtgtccgag tggtttaagg agctggtctt gaaaaccagt gacttcgaaa
     1201 ggggccgtgg gttcgaatcc caccctctcc gccaaaaaaa tgactgaatt tggagaagta
     1261 ctcaagtggc tcaagaggat cccctgctaa gggattaggc tgggtaaccg gtgcgagggt
     1321 tcgaatccct ccttctccgc cattcaaaaa cgaggtgtag cgcagtttgg tagcgcacat
     1381 ggtttgggac catggggccg ggggttcgag tcccttcacc tcgaccaact taagctttta
     1441 gttcagttgg ttagagtgcc caactcatgg ctagtaattc tagggtttga gtccatgaag
     1501 gttattcttc ataggggtgt agctcagttg gtagagcgtc ggtctccaaa accgtgcgcc
     1561 gggggttcga gtccctccac ccctgccaaa taaaaaggac ttgaaaaaag ttggatttta
     1621 gagtagtata agatagtaaa taagatttcg aggtgtagcg cagtttggta gcgcacatgg
     1681 tttgggacca tggggccggg ggttcgagtc ccttcacctc gaccaagaaa agggcctata
     1741 gctcaggtgg ttagagcgca cgcctgataa gcgtgaggtc gctggttcga gtccagctag
     1801 gcccaccatt tgcccagata gctcagtcgg tagagcaggg gactgaaaat ccccgtgtcg
     1861 gtggttcgat tccgcctctg ggcaccagga atacggcggt atagcttagt tggctagagc
     1921 gttcggttca tacccgaaag gtcataggtt cgactcctat taccgctacc atctaactat
     1981 atcaataa
//
LOCUS       DAELXX010000233         1944 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.236, whole genome shotgun sequence.
ACCESSION   DAELXX010000233 DAELXX010000000
VERSION     DAELXX010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.236"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            138..1187
                     /locus_tag="KPH29_003703"
     CDS             138..1187
                     /locus_tag="KPH29_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG1469240.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKDESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDVQFGVMAGAFAGGEGDFTTAFEPTATAMEKEGS
                     GYIVTSIGKDSGEIPFTAYSTTRSYMKNNKDLIQRFTNAVYKGQLWVQQASSKEVAKA
                     MQPFFEDISLEDLTTVVDRYKSIDAWSQNPVLKEEGLKKLMTVMKEAKELDKEAPYKD
                     IVNTEFANNAIKNIK"
     gene            complement(1337..1684)
                     /locus_tag="KPH29_003704"
     CDS             complement(1337..1684)
                     /locus_tag="KPH29_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBG1469241.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
BASE COUNT          742 a          249 c          290 g          663 t
ORIGIN      
        1 aaaaaaacta aacataaaac tgaaaattat tattaagttt ttgtagaaat attataagca
       61 tagatacatt tttgtaacaa ctacaataaa tttttaatac tatatactat tcgtatgtct
      121 tatgggaggt atttattatg tttaaaaaaa tagtcgcaat agcaagctct atcattgtaa
      181 gtacaatatg tctaactgga tgtgatttta actctaatga caaaaaagat gaatcttctt
      241 cgtctaaaaa tctggataaa gtaactatag cagaagttac tcactctgtt ttttatgctc
      301 cacagtatgc tgctattacg aaaggattct ttgaagatga aggtataaag attgacctta
      361 taaataccca aggtgcagat aaaactatgg cagctttgat ttcaggtgaa gcaaacgtag
      421 gtttaatggg tcctgaagca tctatatatg tatataatca aaacaaaaac gattatgcag
      481 tgaactttgc tcaattgaca aaaagagatg gtagttttct agttgctagg aaaaaaatgc
      541 ctaatttctc ttacaatgat ttaaaaggaa cagaaatatt aggtggtaga aaaggtggag
      601 ttccactaat gacttttgaa tatgttctaa aagaaaaagg tcttactatt ggagaaaatc
      661 taaaagcagg caatgttaat gtaagaactg atgtacaatt tggtgtaatg gctggagctt
      721 ttgcaggtgg agagggtgac tttacaacag catttgaacc tacagcaaca gcaatggaaa
      781 aagaaggtag tggatatata gttacatcta taggaaaaga ttctggagaa atacctttta
      841 cagcatactc tactacgaga tcatacatga aaaacaataa agatttaatt caaagattta
      901 caaatgctgt ttataaaggt caattatggg ttcaacaagc atctagcaaa gaagtagcta
      961 aggctatgca acctttcttt gaagatatat ctttagaaga tttaacaaca gttgtagata
     1021 gatataaatc aatagatgca tggtctcaaa atcctgtatt aaaggaggaa ggtttgaaga
     1081 aattgatgac tgttatgaaa gaagctaaag aactagataa ggaagcacct tataaagata
     1141 tcgtaaatac tgagtttgca aacaatgcta taaaaaatat taaataaaat atattagttt
     1201 tagctagata ttttcttgtt tttataatga aaattaacaa aaatataaaa agagcctttt
     1261 aaaagactct ttttatattt gagattaaag tttatataat aaaatagata ttaaagtact
     1321 aactgtactt atatatctat cctctgtatt ctgctccagc attaattttt gtaatttgaa
     1381 cggctaccat ttcttcattg cttccattag taatttcatg ccactctcct gttggtacaa
     1441 atacagtatc gccgtttttt acatcacatt catgctcacc aagtttcatt ttaccttctc
     1501 cttttaggaa cacaaatatt tgttcaccat tagggtgtct atgagcatta agaacttgat
     1561 taggtttaaa ataataaact tctgaagtta tatcgttacc agcaaatact ggtatttttg
     1621 gaactgctgt atcattatat tgagctaagc ttccgtctaa tagattcttt ttattttcat
     1681 gcatactaga aatacctctt ttctatatta tagatttact attattatac caatataaga
     1741 aaatagtatt ctcactttac acaataggaa taaaattctt ttatttttaa atataagtaa
     1801 atactatttt tatagttaga aaaatataat cattataaga tataaaaaaa ttaacataat
     1861 ataataaatt ctaaaagtat ttttagatat ctaaacttta atataaagaa tatatgaata
     1921 ttttttataa aaaattaaaa ttat
//
LOCUS       DAELXX010000234         1941 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.237, whole genome shotgun sequence.
ACCESSION   DAELXX010000234 DAELXX010000000
VERSION     DAELXX010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.237"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            204..398
                     /locus_tag="KPH29_003705"
     CDS             204..398
                     /locus_tag="KPH29_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HBG1469242.1"
                     /translation="MSVIVMCSRCGKEIDKYRNKYITYYEKNTNAEIKVCINCALDLL
                     EKTRENQELNIREFQDTIIL"
     gene            complement(500..1009)
                     /gene="vanZ1"
                     /locus_tag="KPH29_003706"
     CDS             complement(500..1009)
                     /gene="vanZ1"
                     /locus_tag="KPH29_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ1"
                     /protein_id="HBG1469243.1"
                     /translation="MKSRKHNITKGLFIVYIIILTWIILFKLQFDISSLETMNLRSIN
                     LVPFAGSLIINNRVDISEIILNVAIFVPFGIYVCMLKEEWSFIKKVIPIFITSLAFET
                     LQYIFALGASDITDLIGNTLGGIIGIAVFMLLSKIFKNNTIKIINVLALIVTIIVVLF
                     LGLVIFANL"
     gene            complement(1329..1719)
                     /locus_tag="KPH29_003707"
                     /pseudo
     CDS             complement(1329..1719)
                     /locus_tag="KPH29_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902377.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT          720 a          262 c          194 g          765 t
ORIGIN      
        1 tgatgaaaaa taataaaatt ttataatcta ttcaataata tacttttttc tataaatatt
       61 ttacccataa cctttatttt tttattaaaa tgcattataa taaaaagtaa atctaaattt
      121 gatacagttt taacaatcat tattttcaga aaagtgatac tattgaatat attaagaata
      181 attagagatg gaggaactaa gatatgagcg taattgtaat gtgtagtcgt tgtggaaaag
      241 aaatagataa atatagaaat aaatatataa cttattatga gaaaaatacc aatgcagaaa
      301 ttaaagtttg tataaattgt gcattagatt tattagaaaa aacaagagaa aatcaagaat
      361 tgaacatacg agagtttcaa gatacaataa ttctttaata atttaggaaa gcacctatat
      421 aggtgctttt tatgtgtaaa taattttata tgtaactgta tcttacgttc tattttaagc
      481 taaacctata tacttatttc tataagtttg caaaaataac taatcctaaa aataatacaa
      541 caataattgt cacaatcaat gccaatacat tgataatttt tatggtatta ttcttaaata
      601 ttttcgataa tagcataaaa acagctattc ctatgattcc tcctagtgtg tttcctatca
      661 aatcggttat atcacttgca cctagtgcaa atatatactg aagtgtttca aatgctaaac
      721 tagttataaa aataggtata acttttttta taaagctcca ttcttctttt agcatacaca
      781 catatatccc aaatggtaca aatatagcca catttaatat aatctctgat atatctactc
      841 tattattaat tataagtgac ccagcaaaag gtactaagtt tatgcttcta agattcatgg
      901 tttcaagaga tgagatatca aattgcaact taaatagtat tatccatgtt aaaataatta
      961 tgtaaactat aaataatcct tttgtaatat tatgttttct agatttcatt tattacctcc
     1021 ttactatatt attgtattat tttattaata caataatata gtaaggtaat ttatctgtca
     1081 actatttctt ctaaaattat attcctttta taatttcaat atctttatca tatttacatg
     1141 atttaagcta ttgtagtatt tcatatatct ttaaatttta attggtatta ttctctatat
     1201 tttttactat caaacactaa ggaaataatc acttttcttt atataatgtt ttaccatcac
     1261 ataaaactaa cattaaatcc aaacgctact ctcaattgtc tcaaactaaa aagatactga
     1321 tagctttatt agtcatcaat atcttcttct ttcaaatttt taaagtattc ttctaatttg
     1381 tccaagctct tactatctaa aacttcatca tcatgtagtg ctgaaataaa actcttaaat
     1441 gaattgttat gcataaaact aaaaaaactc tttgtctcaa cttttaagta atctttctct
     1501 tttatagcta tagtataatg cgtacatcta tctaccttaa taagtagtta aaaatccctt
     1561 atctactaaa gtttttaaaa atgtcaatgt tgtgctcttt ttccaatcgt atttcaactt
     1621 cattgcttct atgatatctc ttgaggctaa tacatcatct gctccccaaa tatacttcat
     1681 tagttttagt tctgcatgtg gtaatttatt aatcttcatt agtttacacc tcttaccaat
     1741 ttatgtataa ttaaatttta tactaataat agtatgtaat caatcagatt ggaacaaata
     1801 taatattcca atttattcca tataaaaata attaataaaa aagaagtact ctactttaga
     1861 gagtactttt ggattatata ttcaaaaatt tatctaacat gattagagta tgttttatgc
     1921 tttagtgtga taattttagt t
//
LOCUS       DAELXX010000235         1936 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.238, whole genome shotgun sequence.
ACCESSION   DAELXX010000235 DAELXX010000000
VERSION     DAELXX010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.238"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            351..1643
                     /gene="brnQ"
                     /locus_tag="KPH29_003708"
     CDS             351..1643
                     /gene="brnQ"
                     /locus_tag="KPH29_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBG1469244.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLI
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIKFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGVK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT          726 a          220 c          325 g          665 t
ORIGIN      
        1 atgtaaaaag gagagtgata tatacagtat ataaagcaat acaacttaat aataaaacga
       61 ataatctaaa aagtcttaat attttaattg ttctgaatag ttaattttat caaggaaaat
      121 tagagaaaaa acaaaaaacc aactatgttt aaagtataca ggttgatttt gtttaattaa
      181 gaaagttgta ttaaatattg tgaagatttt ataaattatg aaacataatt gacaaattta
      241 agtcaaaaat gtataattaa ataaaatctc gtcaaaaggt tttcatttaa tgaagagatt
      301 ggacttattt tttagtaaaa ggtagtaaaa ttcaaatggg ggtgctttaa atgggtgaga
      361 aacaaatcaa ttttaaagat gtaattgtaa ttggatttgc actatttgca atgttttttg
      421 gtgcagggaa cttaatcttc cctccatatc taggagtttt atctggaagt agttggctaa
      481 tagctttcat aggattcctt tttgcagatg gtggtttggc attattagca gttatagcag
      541 caactaagtt caatggagac acatcgaaga tgttctcaag agcaggcaaa ggattaagta
      601 ttgcattagg atgtgcaatg gtaatctgta tagggccact tttagcaata cctagaacag
      661 cagcaacaac ttatgaaatg ggtatattgc ctacaatagg ttctggaata agtccagtaa
      721 tattctctat agtattcttt gctctagtat tggttttaac aataagacca tcaaaagttg
      781 tagatatagt tgggtcaata ttaactccag ctttacttat agctttagca gtattaatag
      841 ttaaaggtat cgtatctcca ttaggagaaa taagagacag ttctttaatt caaaatgtat
      901 ttgcagaagg tataactcaa ggttatcaaa caatggatgc attggcagct agtgtatttg
      961 catcaattat aatcatgtca gtaatagcta aaggttatac aggtgagaaa gaaaagatga
     1021 aagccacaat aagtgcaggt gtaattgcag taataggaat ggcattagtt tatggtggat
     1081 tatgttatct tggagcaact gtgtctcaac aatatggaca agatgttcaa caaacagctt
     1141 taatagttgc aataacagca gctttacttg gaactacagg taaaatttta ttggctatta
     1201 tagttgcatt agcatgttta acaacagcta taggtctttc ttcagcagct ggtcaatatt
     1261 tctcaacact tacagatgga aaattaaagt atgagcatat agttatagta gtttgtgtat
     1321 ttagtgcaat catatcaaac tttggagtaa gtacaataat aaaattctct tctcctatac
     1381 taagtatggt atatccagca actataactc tagttatact agcattattt agtaataaaa
     1441 ttaaaaatga caacgtgttc aaatgtgctg catacatggc attattagta agtgtattaa
     1501 cagttgcaac aagttttggt gtaaaaattc ctctagttaa tagcttacca tttgcttcat
     1561 taggatttaa ctggatagtt ccagttgtaa tagcaggaat aataggaaac tttattccat
     1621 ctaaaagcag taaaactgca taataataaa actaattgat aattgtggaa ttgcctttaa
     1681 attatatggc aattcctttt tatatttaaa agaattatta aattattgtg gagaaaagaa
     1741 cattattaat ctcatttgat aaatttattt ttggaggagt atgatttata aagacaataa
     1801 aataaaaaag atttatttat aattataaat aaaattaaat tatatattat gaatgtttaa
     1861 tattaatata aaaacatcaa tatatatttt tgtaaagaaa aaataaagaa tttattttct
     1921 gaatacttat taatta
//
LOCUS       DAELXX010000236         1882 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.239, whole genome shotgun sequence.
ACCESSION   DAELXX010000236 DAELXX010000000
VERSION     DAELXX010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.239"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            527..1798
                     /gene="serS"
                     /locus_tag="KPH29_003709"
     CDS             527..1798
                     /gene="serS"
                     /locus_tag="KPH29_003709"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBG1469245.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKDFNLDEVVKLDDERRKILQE
                     VEVMKHKLNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGLDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVVVPEVLRPYMGVSVIK"
BASE COUNT          798 a          195 c          314 g          575 t
ORIGIN      
        1 aatagcaaat ttaaatttat taaatagatt accttataac atttcatata aaatatattg
       61 tgaatctgag aaaattacta tttttctttg agtatttttt ataagcagtt ctaaaagatt
      121 caatttcata cctattactt gaagcattca ttaaaataat tctaatttaa aaaataattc
      181 tatccaaata atagatatat aggactatgt tataatataa aaaaatttat ataaaagtta
      241 ataactatac aagattgata aagaaaaagt caaaatagat aattgattag caaaatagat
      301 ataattataa tgaagttaaa tctagcaata ttctcctaaa attaaatttc atttatttaa
      361 ttaaatataa tttgacaatg gacaaaaaag tgagtaaaat ataaataaaa taaaaataac
      421 aggttcggat aaagaaaaag gtttcttaac ctcaagcata aagtatatta tctaagttat
      481 ttgagattaa gagataaatc caatcttagg tactgaggag gaaaaaatgc ttgatataaa
      541 aagaataaga gaaaatttag attgtataaa aaaagcaatg gaaagaaggg gcgaaaaaga
      601 ctttaacctt gatgaagtag ttaagttaga tgatgaaaga agaaagattc ttcaagaagt
      661 tgaagttatg aaacataaat taaatacagc atctaaaaat ataccaaatc ttataaaaga
      721 aggtaaagat gtagaaaatg aaaagattaa actaaaagag ttatctgata aaattaaggt
      781 aatagaccaa aatcttaaag aagtagaaga taagatggaa tacctattaa tgagaatacc
      841 aaatgtacca catccagaag taccacaagg tgaaactgat gaagataatg ttgaggtaag
      901 aacttgggga aagactacta cctttgattt tgaatctaag gctcattggg aaattggaac
      961 agaattaggt atacttgatt ttgaaacagc gagtaaaata actggttcaa gattcactct
     1021 ttacaaagga ttgggtgcta gattagagag agcattatta aatttttact tagacacgaa
     1081 tacaaaagta aatggatata cagaagtcat tccaccattt atggcaaata gaaatagttt
     1141 tttaggtaca ggacagttac ctaagtttga agaagatatg tttaaaatag aaggtctgga
     1201 ctattttatg attccaacat cagaagttcc tttaactaat atacatgcta atgaaatact
     1261 taaatttgaa caattaccaa taaattatac agcttatact ccatgtttta ggtcagaagc
     1321 tggttctgct ggaagagata caagaggttt agtaagacag catcagttta acaaagtaga
     1381 aatggttaaa atagttgcac cagaagaatc ttacaatgag ttagaaaagc ttactaacaa
     1441 tgcggaaact atgctacaat tattgaatct gccttataga gtagtaaaga tatgtacagg
     1501 tgatttagga tttacggctt cttttaaata tgatgtagaa gtttggatgc caagttacaa
     1561 taggtatgtt gagatttcat cttgttcaaa ctgcgaagat ttccaagcta gaagagcagg
     1621 aataagattt aaaagagata aagattctaa agctgaatat gtacacacat taaatggctc
     1681 tgggttagct attggaagaa gtgttgcagc tatattagaa aattatcaac aagaagatgg
     1741 ttctgtggtt gttcctgaag ttttaagacc atatatggga gtttctgtta ttaaataaga
     1801 gttaaattat gtatttttaa gcaatacaat aatatagaag taataaaaaa taattaatat
     1861 aaaagtgata caataatata ga
//
LOCUS       DAELXX010000237         1771 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.241, whole genome shotgun sequence.
ACCESSION   DAELXX010000237 DAELXX010000000
VERSION     DAELXX010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.241"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            283..738
                     /gene="smpB"
                     /locus_tag="KPH29_003710"
     CDS             283..738
                     /gene="smpB"
                     /locus_tag="KPH29_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBG1469246.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
     gene            896..1245
                     /gene="ssrA"
                     /locus_tag="KPH29_003711"
     tmRNA           896..1245
                     /gene="ssrA"
                     /locus_tag="KPH29_003711"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          674 a          205 c          312 g          580 t
ORIGIN      
        1 atttataata tgtaatattt ttgttttgaa atttagaaat cagtgttact ggttaaagtt
       61 agtttagtat taagtatatt cgaagtagtt ttttagctat tttcatatct ttatattact
      121 taattatata tagcctaact aacttaatat atcagtgaaa tattgactaa aatgcctaaa
      181 tatgttatca tacaagttag tcaaataatt tggcaacatt aaatatttag taaatttcta
      241 acatttatgg aataaatttc taacaaagaa ggtgagattt atatggctgg taaaaaaata
      301 ttagcaacaa acaaaagagc aagacatgaa tattttatag aagaaactta tgaatgtgga
      361 attgaattaa aaggaacaga agtaaaatca ataagacaag gcaaagttaa cctaagtgat
      421 ggatttgcat ctgtagataa tagtgaagta tttttaaaac aagttcatat aagtccatat
      481 gagcaaggaa atatattcaa tgttgattct ttaagagtta gaaaactatt acttcataag
      541 catgaaataa gaaaacttat tggagcaact actataaagg ggtattcact tataccactt
      601 agtttgtatc taaaaaacgg taaagttaaa gtagagattg ccttagcaaa aggtaaaaaa
      661 ctatatgata aacgtcaaga tatggctaag aaagatgcac aaagacgtat agaaagagaa
      721 atgacaggta agtattaaac tagataaaac tgtatttttt agaataaaat tctttattat
      781 ctagaacata acattgattt ttattcttaa aagtattata ttatatatac attaaaaatt
      841 taataaaaat ggtgataaag tttgcgaaaa gtctactgga ctataaacta ctcaaggggg
      901 cgtaaaggtt tcgacgtggg tttggaactt gaggctgcat gtcgtgttac tctgggtcac
      961 gtaaaaactg gggaacttta aaataaacgc agacgataat ttcgcgatag cagcctagtt
     1021 gctgctcgtc cctcttagtc ctcctgccga ctaagactgg acgtcattat gcagggaact
     1081 actttagggg tgtctcgacc tgaagtagat taattgggac tggctaagca tagagcctgc
     1141 cacttggcgc tatgtgaggt gagatattag tgaagttggc taagcatgta gatgcagcaa
     1201 ggaaaagatt tgcggacagg ggttcgactc ccctcgtctc caccaatttt taaaaaagaa
     1261 tgatgaaata cttggaaata ctagtcttta ttgactagtg ttttttgttt tggagatgaa
     1321 atccaccaac cgtgccccaa ccacagaaaa agagattttt ttggggtata ttggggtgca
     1381 tttgattaat ccatggaaca tataaagaat acttttagag atatagtgaa tttgatgata
     1441 atacaataaa tattttatac tgatgaattg tttttatatt atgcttttta tttcaatcta
     1501 ggaaatatat aacttgttta aacttatgat taaaaggata aagaagttta aagcatataa
     1561 gtgctactta atctattaga gtaattacta gttataatga tatacagaat cctataagat
     1621 tatgtataag tatagaacaa atttattatc tatgtgggat gctaatttaa attttttaat
     1681 tgtatttaaa aaaataagat gtaaaaaagg aattgaaaaa tgttaagata ataaacacat
     1741 agaattatta agtttaatac taacaagact a
//
LOCUS       DAELXX010000238         1582 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.242, whole genome shotgun sequence.
ACCESSION   DAELXX010000238 DAELXX010000000
VERSION     DAELXX010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.242"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            256..684
                     /locus_tag="KPH29_003712"
     CDS             256..684
                     /locus_tag="KPH29_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG1469247.1"
                     /translation="MYGVIGATVLVIFVILTAGIMQGKKVDFQLPTVFMIVTFIVSAI
                     SEEIVFRGYIQTRLTGLIKNSVLSSCINAFLFLSTHYPVKWISSGDFSFAILSGFYVI
                     CLILLNFSCDLVYRKTNCLWGAWLLHILYNIGTGVLIFTT"
     gene            804..1412
                     /locus_tag="KPH29_003713"
     CDS             804..1412
                     /locus_tag="KPH29_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBG1469248.1"
                     /translation="MVDNLIKYIHKSNIAIVVLHEIYGVNNFIKDVCQKYYKGGYDVF
                     CPELLGEKRVFSYLKAQEAYDFFINNVKFDIYKKVEKLINGLKSEYKHVFVVGYSIGA
                     TIAWRCSENSLCDGIICCYGSRIRDYMDVIPTCPILLVFAKYDSFDVDYVSSQLSRRQ
                     NIQIEILDANHGFIDTYSKNYSNAQTEVFNIKEKNFLSKCLG"
BASE COUNT          553 a          162 c          255 g          612 t
ORIGIN      
        1 aaagaatatg ttttattatg taagaattat tctaaaagtg atggagtaaa agcaatattt
       61 atttatttga tatctatgtt actattgttt ttccaaggtt atttgtatac aacaaattta
      121 agttcaacaa ttttaactaa ctcacaaatg atattgtcaa tcttgatttt acttataggg
      181 ctgggatttg tactactttc gaaggaaaaa ctaaatacga ttgggattac aatgtgaaat
      241 ttaaataaaa gtcttttgta tggagttatt ggtgctactg tactcgtaat tttcgttatt
      301 ttaacagctg gaattatgca aggaaaaaaa gttgattttc aattacctac tgtttttatg
      361 attgttacat ttattgtttc tgctatttca gaagaaattg tttttagagg gtatattcaa
      421 acaaggttaa ctggattaat aaaaaatagt gtactttcat cttgtattaa tgcttttctt
      481 tttctatcta cacattaccc tgtaaaatgg atttcaagtg gagatttttc atttgcaatt
      541 ctatctggat tttatgtgat atgccttata ttacttaact tttcatgtga tttagtatat
      601 cgaaaaacta actgtctgtg gggggcatgg ctactgcata tcttgtataa cattggcaca
      661 ggagtgctga tttttactac ttagtcaatt ttactttgca gaaagtttat cgtaagaaac
      721 ggattatttt ataagtcaaa ttttagagct agtaaattga catacttaaa ataatttaaa
      781 ctagatgtaa aaaatatcga caggtggtgg acaatctgat taagtatata cataaaagta
      841 atatagctat tgttgtactg catgaaattt atggagttaa taattttata aaagatgttt
      901 gtcaaaagta ttataaaggt gggtatgatg tgttttgtcc agaactctta ggagaaaaaa
      961 gagttttttc atacttaaaa gctcaagaag catatgattt ttttataaat aatgttaagt
     1021 ttgatattta taaaaaagta gaaaaactta taaatggttt aaaatcggag tataaacatg
     1081 tatttgtagt tgggtatagt ataggtgcaa ctattgcatg gagatgtagt gaaaactctt
     1141 tgtgtgatgg tataatttgt tgttatggct cccgaattag agattatatg gatgtaattc
     1201 caacatgtcc tattttgcta gtgtttgcaa aatacgattc attcgatgtt gattatgttt
     1261 cttctcaact ttctagaagg caaaatattc aaattgaaat tttagacgca aatcatggtt
     1321 ttattgatac atactcaaaa aactattcta atgctcaaac agaagttttt aatattaaag
     1381 aaaaaaactt tctatctaaa tgtttaggct aatatgtttt attataaaat atagaaaatt
     1441 taatcaattt gaaaaacata atgacactac taatgttaaa aaatattagt agtgtcatta
     1501 tattttttga gatagagata gtcagtagaa tcatagaatt aattatgttt ttatgattgt
     1561 attagattaa tatttaaatt ac
//
LOCUS       DAELXX010000239         1536 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.243, whole genome shotgun sequence.
ACCESSION   DAELXX010000239 DAELXX010000000
VERSION     DAELXX010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.243"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            240..1067
                     /locus_tag="KPH29_003714"
     CDS             240..1067
                     /locus_tag="KPH29_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBG1469249.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGNGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
BASE COUNT          581 a          166 c          222 g          567 t
ORIGIN      
        1 ataattttaa catgcccata aaatataata cccacaaatg ataaataata ctcaaaatat
       61 ctattgaaat aactatacat ccttaaataa cttaacttaa atctaaacat ttcaaaaact
      121 tcttaaaaat atgtaaaaat ttgatgataa agtagcgatt ttttcaaaaa aaatgtaata
      181 tagagttata tcttataata ttttagagat aaattttatc atagttggga gaaatttgta
      241 tggatttaag caaaaaagta aaaattttag aaaatgcatc agatggcaaa agcgaatttg
      301 aaatattaga atttgaaaaa cttgagggag cttctagccc attaatggca atgggattat
      361 attttgctag agaagctggt ttgaaagcta gacaaattaa gataaaatta aataattcat
      421 caataaaaac agaagctggt gcattatact attataaagg aaatatatct tctgaaacta
      481 agattggtgg tgttggaggt ctttttaaaa aaggaatatc aggagcactt actaatgaat
      541 caatgatgaa acctgtatac aaaggtactg gagaagttta tttggaacct tcttttagac
      601 attatttatt tatgagtctt gataatgatt ctataattgt tgataaagga ttattctatt
      661 gttgctctga tacaatagaa gtaaaagcat ttacacaaaa gaacttctca tctgctttac
      721 ttggtggtga aggcttcttc caaatagaat taagtggtac tggtgtaatc gttctggaat
      781 gtgttgtacc tcaaagtgaa attgtagagt atgaattgaa aaatggagaa gaattaaagg
      841 tagatggtaa ctttgcaata gcaagaacat ctggggttaa tttttctgtt acaaaatcag
      901 ataaatcttt aattggttct gctttaaatg gtgaaggttt cttaaataca tttactggta
      961 atggtacagt ttggctagct ccaactcaag taatgtatga aaaactaagt tttggagcaa
     1021 tgcctactaa tgataatatg aataataaag tttctaagca aaaatagttg ttttataaat
     1081 atttattaaa tcattattga ttttataaag tcactataaa ttttattaga ttattattat
     1141 aaaatcagag tgtggaaatg tttatgtgta ttctttaaat aaaaatttcc acattctgta
     1201 aggttattat acttgatata taaaattcaa tttaggaaca tagtctaaca tttataaaca
     1261 tattaaggta atgatttctc ttggtaatta ttctcatatc ttattctttg attatattta
     1321 tcttattcct agcccttatg tcatattata tttcttatct tattatttat aattagcatt
     1381 aacttatcta aaatcttgta tttttttaat agtaaagttt acatctatat taaaaagttt
     1441 acatctatat taatttcata aaacaatcaa ggtagtcaag agattcaact ttatttgatt
     1501 ttaaaaattt aagtgaattt gttcttatac acatct
//
LOCUS       DAELXX010000240         1455 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.244, whole genome shotgun sequence.
ACCESSION   DAELXX010000240 DAELXX010000000
VERSION     DAELXX010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.244"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(308..820)
                     /locus_tag="KPH29_003715"
     CDS             complement(308..820)
                     /locus_tag="KPH29_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1469250.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KRDFQEDYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKAKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
BASE COUNT          482 a          231 c          110 g          632 t
ORIGIN      
        1 tttttgacgc aatcgttttt attcagtcat tttatattct tgatacaatc attcttacat
       61 tctattgttg tttgttttta acacaatcgt tttttgttga atcattttat attcttgata
      121 caatcattct tacattctat tattgtttgt ttttgacgca actatttttt attcagtcat
      181 ttaatatttt cgacacaatc attcttacat tctattattg tttgtttttg atacaactgt
      241 ttttattcag tcatttaata ttctcaacac aattaccttt tcattcaatc aaagtaatct
      301 ttaagcatta tttttcaagc tcatcaaagt aattatatac gataacctca cctgtctctt
      361 tatctgtagt ctttataatg cccttagctc catttacctc ttcttttgta aattcatcat
      421 ctaacacaac tttgaattta tcttcctttc cataaccttg ttgaaattca tctctcaaaa
      481 ttgcattttt ttctgctttt gccttcaact caaattcaat cccagatttc atttcatcaa
      541 aataatttaa tactacaacc tcattattca ttttattagt tgtctttata atccctttag
      601 ctccatttac ttcttctttt gtaaattcat catccaatgt tatattaaat ttgtcttctt
      661 ttccataatc ctcttgaaaa tccctcttca tttcattgta tttagtttca aactctttac
      721 tcttgtttaa ttccatagct gatacatttg tcaatgatgc tccaattgcc attactgtta
      781 atgccattac tataccactt ttaaattttt ttactttcat accttacctc ctaaattaaa
      841 tgttctcaat gaaattatat aaagaaaatc ctaagtttta cagtttcatt cttgtaacta
      901 aaattactat tatgtaacca aactttttgt ttcaactatt ccttaaaagt tgctttttta
      961 aggaatagtt gaagttaaat aataagattt tatctatatt gatagattta aaatcaaatt
     1021 tatccatata aataaatact tatttttgca aaataaaaat ttttaattac tccaatcctt
     1081 cttatttact taaaatcctg tgataaaaaa tttaaaagtc tttacattct ttaacaataa
     1141 tttatttact tttaacatac aaaatatata gagtatatca aaattatttt actgaatatt
     1201 ctaaaaataa gtacccccta ttagtatata tatttatata ttaaaaaaaa gttttaaata
     1261 aagttatatt ttttacaaca gttttgtaat taaaaacata ttgaaggtaa atttaaaggt
     1321 actatatact ataaatatgt cgacacaata aatttaaaaa ggagaactaa atatgaaaaa
     1381 aagattaata ataatgatat tgtctgttgt cttagtacta tcaagtatac ttacgatatt
     1441 tgcatattca aatat
//
LOCUS       DAELXX010000241         1410 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.245, whole genome shotgun sequence.
ACCESSION   DAELXX010000241 DAELXX010000000
VERSION     DAELXX010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.245"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..829
                     /locus_tag="KPH29_003716"
     CDS             <1..829
                     /locus_tag="KPH29_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HBG1469251.1"
                     /translation="KAVNKIYEENEKWKEYNIQNTMSKYIGIPESEVIFISNLSQYVS
                     FLSELDNAGDYVSRGQKDCMYELLPSLHRKYKKDYGIHSDEYEEAFKQKIIYYDKEIK
                     NRSSEELRAEGQHFGLPTEYLDFTEAHLISLLFAIEDYKYTKQHSIVYFIDSISYNTD
                     TIKRSEKLVNYSDTSAIDNCSKFSSRSFFIKVGNSNERIHFQKGCFLKVSSEDKGEFK
                     EKLKKYCKVVVINKDAKKEILKELFNLGITFENIYPDKDNVVKSIKFYYDEIVGGNL"
     gene            829..>1410
                     /locus_tag="KPH29_003717"
     CDS             829..>1410
                     /locus_tag="KPH29_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBG1469252.1"
                     /translation="MREQFKNSIKNIKSNRPLVSVQDIVNFIDKGEKYLKLSIDEIDE
                     MEILQGIVMSPDYQRTYKSSNKEESSIIESLLIGIPIPEIFLVKIGVNDMQIRHVMDG
                     QHRLNAIYRYVKNKYPLKNLDILGNDPLYDNKRFSELEKKDKIKILGSHLSILEFDGF
                     DNPEIEIELFKRYNRNTKPLEMQEIEMATYFSET"
BASE COUNT          622 a          136 c          216 g          436 t
ORIGIN      
        1 taaagctgta aataaaatat atgaagaaaa tgaaaagtgg aaagagtata atatacaaaa
       61 tactatgtct aaatatatag gtatacctga atcagaagtt atatttatat caaatttgag
      121 tcagtatgtt tcatttttat ctgaactaga taacgcaggc gactatgtat ctagagggca
      181 aaaagattgt atgtatgaat tattaccatc attacataga aaatataaaa aagactatgg
      241 tatacattca gatgaatacg aggaagcatt caaacaaaaa ataatttatt atgataaaga
      301 aataaaaaat agatcgagtg aagaattaag agctgaaggg caacattttg gactaccaac
      361 agaatattta gatttcacag aagctcattt aatatcatta ttatttgcaa tagaagacta
      421 taaatataca aaacagcatt caattgtgta ctttatagac tctatttctt ataatactga
      481 cactataaaa agatctgaga agttagtaaa ttattcagat acatctgcaa tagataattg
      541 tagtaaattt tcatcaagat cattttttat aaaggtaggg aattcaaatg aaagaataca
      601 ttttcaaaaa ggatgtttcc taaaagtatc atcagaagat aaaggcgaat ttaaggaaaa
      661 attaaaaaag tattgtaaag tagtagttat taataaagat gctaaaaaag aaatattaaa
      721 agaattattt aatttaggta ttacatttga aaatatttat ccagataaag ataatgtagt
      781 taaatctata aaattctatt atgatgagat agtaggggga aatttataat gagagaacaa
      841 tttaaaaatt ctataaaaaa tattaaatca aacagacctc ttgtatcagt ccaagatatt
      901 gtgaatttta ttgataaagg agaaaagtac ttgaaattat caatagatga aatagatgaa
      961 atggaaattt tacaaggaat agttatgagt cctgattatc aacgtacata taaatcatca
     1021 aacaaggaag aaagttctat aattgaatct cttttaattg gaataccaat acctgaaata
     1081 tttcttgtaa aaataggtgt aaatgatatg caaataagac atgtaatgga tgggcagcat
     1141 cgtttgaatg ctatatatag gtatgttaag aataaatatc cacttaaaaa tttagatata
     1201 ttaggcaatg acccactata tgataataaa aggttttcag aattagagaa aaaagataaa
     1261 attaaaatat taggaagtca tttatcaatt ctagaatttg atggttttga taatccagaa
     1321 atagaaatag aattatttaa aagatataac agaaatacaa aaccattaga gatgcaagaa
     1381 atcgagatgg caacatattt ttcagaaact
//
LOCUS       DAELXX010000242         1336 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.246, whole genome shotgun sequence.
ACCESSION   DAELXX010000242 DAELXX010000000
VERSION     DAELXX010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.246"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            27..101
                     /locus_tag="KPH29_003718"
     tRNA            27..101
                     /locus_tag="KPH29_003718"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="KPH29_003719"
     tRNA            116..200
                     /locus_tag="KPH29_003719"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            209..292
                     /locus_tag="KPH29_003720"
     tRNA            209..292
                     /locus_tag="KPH29_003720"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:243..245,aa:Leu,seq:tag)
     gene            322..398
                     /locus_tag="KPH29_003721"
     tRNA            322..398
                     /locus_tag="KPH29_003721"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Arg,seq:tct)
     gene            406..481
                     /locus_tag="KPH29_003722"
     tRNA            406..481
                     /locus_tag="KPH29_003722"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:439..441,aa:Gln,seq:ttg)
     gene            570..658
                     /locus_tag="KPH29_003723"
     tRNA            570..658
                     /locus_tag="KPH29_003723"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:604..606,aa:Ser,seq:tga)
     gene            662..737
                     /locus_tag="KPH29_003724"
     tRNA            662..737
                     /locus_tag="KPH29_003724"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:695..697,aa:Phe,seq:gaa)
     gene            744..817
                     /locus_tag="KPH29_003725"
     tRNA            744..817
                     /locus_tag="KPH29_003725"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:778..780,aa:Met,seq:cat)
     gene            832..908
                     /locus_tag="KPH29_003726"
     tRNA            832..908
                     /locus_tag="KPH29_003726"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:866..868,aa:Ile,seq:cat)
     gene            932..1008
                     /locus_tag="KPH29_003727"
     tRNA            932..1008
                     /locus_tag="KPH29_003727"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:966..968,aa:Pro,seq:tgg)
     gene            1016..1092
                     /locus_tag="KPH29_003728"
     tRNA            1016..1092
                     /locus_tag="KPH29_003728"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1050..1052,aa:His,seq:gtg)
     gene            1101..1176
                     /locus_tag="KPH29_003729"
     tRNA            1101..1176
                     /locus_tag="KPH29_003729"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1134..1136,aa:Lys,seq:ttt)
     gene            1184..1257
                     /locus_tag="KPH29_003730"
     tRNA            1184..1257
                     /locus_tag="KPH29_003730"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1216..1218,aa:Cys,seq:gca)
     gene            1264..1335
                     /locus_tag="KPH29_003731"
     tRNA            1264..1335
                     /locus_tag="KPH29_003731"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1296..1298,aa:Asn,seq:gtt)
BASE COUNT          320 a          310 c          355 g          351 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atgtaaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaatgcgc ggatgtggcg gaattggcag acgcactaga
      241 cttaggatct agcgccattg gcgtgggggt tcgactccct tcatccgcac catttttgtt
      301 tgatatacat atataaattg tgggtgcata gctcagctgg atagagcaac gcccttctaa
      361 ggcgtgtgtc cggggttcga atccctgtgc gctcaccaca tatattgggg attagccaag
      421 tcggtaaggc acatgacttt gactcatgta tgcgtaggtt cgagtcctgc atccccagcc
      481 aaatgatgta ttactaagct atatttgaac tatttttatt ttaaatatag ttaggttcaa
      541 ttttagaata agtttaagta tccgaatgtg gagaggtgtc cgagtggttt aaggagctgg
      601 tcttgaaaac cagtgacttc gaaaggggcc gtgggttcga atcccaccct ctccgccaaa
      661 tgcccagata gctcagtcgg tagagcaggg gactgaaaat ccccgtgtcg gtggttcgat
      721 tccgcctctg ggcaccaaaa tatggcggta tagcttagtt ggctagagcg ttcggttcat
      781 acccgaaagg tcataggttc gactcctatt accgctacta aaatttaatg tgggccatta
      841 gctcagttgg ttagagcgcc cggctcataa ccggtaggtc tggggttcga gtccctgatg
      901 gcccaccaca ttaaattaat taatataaat tcgaggtgta gcgcagtttg gtagcgcaca
      961 tggtttggga ccatggggcc gggggttcga gtcccttcac ctcgaccaat ataaggtggg
     1021 tatagctcag ttggttagag cgccagattg tggctctgga ggtcgtgagt tcgactctca
     1081 ttatccaccc caattcatat ggtctattag ctcagtcggt agagcacctg acttttaatc
     1141 agggtgtccc gcgttcgagt cgcggataga tcaccaaaaa ggtggcgaca tagccaagtg
     1201 gtaaggcagt ggactgcaac tccttgatcc ccagttcgaa tctgggtgtc gcctccaaaa
     1261 tattccaagg tagctcaacg gtggagcaac cggctgttaa ccggtaggct gtgggttcga
     1321 gccccaccct tggagc
//
LOCUS       DAELXX010000243         1316 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.247, whole genome shotgun sequence.
ACCESSION   DAELXX010000243 DAELXX010000000
VERSION     DAELXX010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.247"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            218..928
                     /gene="rgaR"
                     /locus_tag="KPH29_003732"
     CDS             218..928
                     /gene="rgaR"
                     /locus_tag="KPH29_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HBG1469253.1"
                     /translation="MFRVVICDDEKIQRSILKEFTKKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNMITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
BASE COUNT          525 a          108 c          198 g          485 t
ORIGIN      
        1 catattatat ttattttact atattttttt acataatata tctagaaaag tataggaatt
       61 tatcgtaatt taagtttaaa tattaaagag aatttctata agtagtttaa tcgacaactt
      121 aaatttataa agaaatttaa atttgaaagc tagtgagtac agtttggaaa ttgaaataaa
      181 gtaaactaaa tgatatttta gatttggagg tgatattatg ttcagggtag ttatatgtga
      241 tgacgaaaaa atacaaagaa gcatactaaa agagtttaca aaaaaacttt tgtctgaaag
      301 atgtctaaat tatcaaatac tggaattttc ctgtggagaa gatttaatat ccaaataccc
      361 agaaaaaatt gatattatat ttttagatat ccaaatgaaa gatatcaatg gaattgaggt
      421 ggctaaaaaa ataagaaagt ttgatgaaaa agtagaaatt atatttacaa cagctttttc
      481 agagtatgcc ccccaaggtt atgaggttcg tgcttataga tatttagtaa agcctataga
      541 gtatagtaac tttgcaatgg gtgtaaatct ttgtattgat aaccttcaaa gaaagaagga
      601 gcgttatata gttttaaata gtaaaaaagg atttaataga atactgatag attctatttt
      661 atatgttgaa actgtaaaga gagatttagt tgtacataca ataggtagag attataaagc
      721 taatatgagc atgaaacaag cagaaaaatt actgtgtgaa aatggctttt ttagatgtca
      781 tacaagtttt attgttaatt taagaagaat agaagatctt agggataata tgattactat
      841 taatgaacaa tttataccta taagtaagta tagacttaaa gatttaaaag tagcattaac
      901 aagcttgtta tgtgatataa tgtattaatt ttaaaaaggt aattgaaaat atcgtaaaaa
      961 agatttaatg tcatcacagg gctattgatt aaaactgtct taatatataa aatgttattt
     1021 atattaagac agttttatta tgcaaaatat cagtttatag acttttaata ttgtttaata
     1081 tgtatatgta gagtttccat tacagtagca tgattattaa ttagtagcct gattattaat
     1141 atactattat tgatttttat atgcattata tgtattttta gaatatgatt acctttttaa
     1201 aataatgact atttataaaa ctctatagat atttgtttca tttagctatg catactttaa
     1261 ttaattatat taatatttaa tggagtataa tataaatatt aaagcgacat cttata
//
LOCUS       DAELXX010000244         1313 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.248, whole genome shotgun sequence.
ACCESSION   DAELXX010000244 DAELXX010000000
VERSION     DAELXX010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.248"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            419..>1313
                     /locus_tag="KPH29_003733"
     CDS             419..>1313
                     /locus_tag="KPH29_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HBG1469254.1"
                     /translation="MMNRLKPRILGISILFIIIIILTCGAVFKFKFKLDNKLKETTHT
                     YLSEFTEHNIINLKTRLSGQFDMLESIANFIGNLNNINNEMVIALMDSDVKRSSLIRM
                     TISTLDGECYSNDGITHNVQDRDYFKKAKNGEKNISEPLKSIVNNKEIIVLSTPVYKS
                     GKIVGVLSGIYDSKKLNELLGLSAFDEKADVFVAKKNGDIVAQKTNKNRKTISCIFQL
                     FDKDNCDEDDCLKTIKENMKNDKSGYFNYNLDNEKFMLNYQPLGVNDWYVFSVVPNKI
                     VSIQSENISYDVYIFALEILLV"
BASE COUNT          546 a          109 c          198 g          460 t
ORIGIN      
        1 attaatctaa aatatatgtt tttatagggt ttaaatttta tgtatacaaa attaatttgc
       61 taactgaatc atataaaaaa aacaaaatat tttttagagg gaatgattag atactatttt
      121 ctttgttatg aaattaattg aagagtaatt aattagaaaa ggaataaata ataatgaata
      181 tattacatta ttactttttg ataaatcaaa aataaagtta tagtttttaa tattaattaa
      241 gaatgatgaa atatgaataa agattttaca aataaatctt aagctatctt atttaattct
      301 aatgcaggaa gaaggtaaat gtatattgaa aaaatcatag aaatagaagt aaacaactta
      361 aacaaataca aaggtttgca aaagaagtaa tataattata tacaatgagg atgtgagtat
      421 gatgaacaga ctaaagccaa gaattttagg tattagtatc ttgtttatta ttataattat
      481 tttaacatgt ggagctgttt tcaagtttaa atttaaatta gataataaat taaaagaaac
      541 cacacataca tatttgtctg agtttacaga acacaatata attaatctca agacaagact
      601 tagtggtcaa tttgacatgc ttgaatctat tgctaacttc ataggaaact tgaataatat
      661 aaataatgaa atggtgattg ctttaatgga ttctgatgta aagcgaagtt ctttaattag
      721 aatgactatc tctacattag atggggaatg ttattctaat gatggaatca cccataatgt
      781 tcaagataga gattatttta aaaaagctaa aaatggagaa aagaatattt cagagccttt
      841 aaaatctatt gttaataata aagaaataat agttttatct accccagttt ataaaagtgg
      901 gaaaatagtt ggtgttttaa gtggaattta tgattcaaaa aagttaaatg agttattagg
      961 attatctgca tttgatgaaa aagcagatgt ttttgttgca aagaaaaatg gagatatagt
     1021 tgctcaaaaa acaaataaaa atagaaagac gatatcatgt atatttcagc tttttgacaa
     1081 agataattgt gatgaagatg attgtctaaa aactataaaa gaaaatatga aaaatgataa
     1141 gtctggatat tttaattata acttagataa tgaaaaattt atgttaaact atcagccact
     1201 tggagttaat gattggtatg ttttttctgt tgtgccaaat aaaatagtgt ctattcagtc
     1261 agagaatata tcttatgatg tatatatttt tgcattagaa atactactag tta
//
LOCUS       DAELXX010000245         1255 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.249, whole genome shotgun sequence.
ACCESSION   DAELXX010000245 DAELXX010000000
VERSION     DAELXX010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.249"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            183..752
                     /locus_tag="KPH29_003734"
     CDS             183..752
                     /locus_tag="KPH29_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1469255.1"
                     /translation="MKLRNPKIKKLLSVALLAALIGIPIGQTANAMEISNNVPSYIEN
                     NPTTIEDLEINDSQLPKPGTVTYIDINKLIKNLKENGIDTTLIENELYYRKAGTNKIV
                     WTKTGFDLYLSKGVALTLAGAGTGVAGLLAGAIPGIGQYLAAAIVGAITMHLGSNITS
                     GKIFKFKKMRYNKGGAYVTKYYLQSVRNQ"
     gene            1044..>1255
                     /locus_tag="KPH29_003735"
     CDS             1044..>1255
                     /locus_tag="KPH29_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1469256.1"
                     /translation="MDFGNINLILIGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIY
                     GNIDKKKTVKLEVLYRYVTGLEYISIG"
BASE COUNT          474 a          148 c          177 g          456 t
ORIGIN      
        1 ctataagctt aaatacagga tatttatttc atatacttaa tttatattaa gttttattat
       61 atgtattctt aagttttact atatttttta ttaatattta atgtagttaa ttcttaagaa
      121 ttatattcta caataataat atatatcttt gatatataac taatttaaaa tggaggtatt
      181 ttatgaaact aagaaatcca aagattaaga aattgttatc agtagcatta ttagcagctc
      241 ttataggtat acctattgga caaacagcca atgcaatgga aatttcaaat aacgtcccaa
      301 gttatataga aaacaatcca acaacaatag aagacttgga aatcaatgac tctcaattac
      361 ctaaaccagg tacagttaca tacatagata ttaataagct aattaaaaac cttaaggaaa
      421 atggtatcga cactactctt attgaaaatg aattatatta tagaaaagct ggaacaaata
      481 aaatagtttg gactaagaca ggctttgatt tatatctttc taaaggagtt gcattaactt
      541 tagctggtgc tggtacaggg gttgcagggc ttttggctgg tgccattcct ggtataggac
      601 agtatcttgc agctgctata gttggagcaa ttacaatgca tcttggttca aatataacat
      661 ctggaaaaat ctttaaattt aaaaaaatgc gttataataa aggtggggct tatgtaacca
      721 aatattactt acaatctgta agaaatcaat aggtgataaa atgattataa aatatctact
      781 ttttgcaatt atctcagcac tagttttatt cttttgtaat aaagtatttc aactaagtaa
      841 attacacaag ttcattatat tgtttgtttc attttttata ctaacttttg tagcatcatt
      901 tattaataga taaaaaataa gatggtgggg ttaacctacc atctttttct catttaatat
      961 ttgtaatatt ttagatactc tttatacttg tgttactgat atactttgat taagaaaagt
     1021 aaatttaaca ggaggtatct gctatggatt ttggaaatat caatcttata cttattggaa
     1081 taatagttat aataggaact acaataatat atttaattaa acctaaaact gctttttgta
     1141 gtaaaaaata ttttaataaa ttagaatcaa tatatggaaa tatagataag aaaaaaactg
     1201 ttaaactaga agtactttat cgctatgtaa caggtcttga gtatatatca atagg
//
LOCUS       DAELXX010000246         1183 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.250, whole genome shotgun sequence.
ACCESSION   DAELXX010000246 DAELXX010000000
VERSION     DAELXX010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.250"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(323..523)
                     /locus_tag="KPH29_003736"
     CDS             complement(323..523)
                     /locus_tag="KPH29_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG1469257.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
     gene            complement(748..924)
                     /locus_tag="KPH29_003737"
     CDS             complement(748..924)
                     /locus_tag="KPH29_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1469258.1"
                     /translation="MFRDKMDKCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(978..1067)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          442 a          167 c          115 g          459 t
ORIGIN      
        1 taattgcttt ttattaacaa attactaaaa gtgttatatc agaattgaat acaaaagaaa
       61 gagtatgtat ttatgagata gttgaaaatt ttgatgtgcc acaactaaaa ttattatatt
      121 actatatatt ctaattaaaa gagtacttgt aggtttaagt acatacttta taaatatatt
      181 tttgtcagat ttattttata aaaagtattt agactaagta ttatttttaa gtattttctt
      241 gttgaagtta ttttttcctt ttttacaaaa tgcgtaaata aaaaaaagac tcttaaaaga
      301 gtctttccga aaaatctata tattataata tagttatatt ttctgcttga ggacctctag
      361 cgcctttaac tatatcaaag cttacttttt gaccttcttc taatgattta aatcctgaag
      421 tttgtatagc tgagaaatga gcaaatacgt catctccact ttccattgat ataaatccaa
      481 atcctttttc attattaaac cattttacta ttccattatt cattaaaaat actcctatta
      541 acctatatca agatatactt gatttacttg tgtattacca caaatactat tatatcatag
      601 attaacataa atgtctattt aattataatt tttagttaac atttttattt attcttaata
      661 tgaaaataaa tttaaacatt ttatattacc aatatattaa tgtaaatgta gataaaacta
      721 aaaagggttt tctagaagaa aatctgtcta attatactta cttaccaata ccatcacttg
      781 atgcaagcaa gattccacaa ctttttctcc gtactctaat ataaaatcat ctaactgtgt
      841 atctataaaa tcacaataat catatgaact accaatataa gcagttagca tatgtgtaca
      901 tttatccatt ttatctctaa acataatgta tttatccccc taaaaaactt tcatgaaaaa
      961 caaaaagaaa atatcttttt ggcaactggc tgacataact tattttataa gcgttagtcc
     1021 ctatagcttt gcgtcactaa atttctctag ttttgccgat ttagttttat tctacagatt
     1081 aataatacaa tagaattagc tattattcaa catatctagc tgaaaaatta ataaatttat
     1141 atatgaacgt taaattattt gaaaaaataa aaagcccctc tgt
//
LOCUS       DAELXX010000247         1159 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.251, whole genome shotgun sequence.
ACCESSION   DAELXX010000247 DAELXX010000000
VERSION     DAELXX010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.251"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            124..615
                     /locus_tag="KPH29_003738"
     CDS             124..615
                     /locus_tag="KPH29_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HBG1469259.1"
                     /translation="MYENNKVIYNFERCEKLENALYLSQFNTYKNFKLTSKKTVINSL
                     DTSTYNMNIHSISLVDTMECISSEDEQMTGKKLILVGDIDTDVTLDYAGNKRNRNSSK
                     KNTFKLKIPFSTFIQMPRKIENKDKINLKYLIQDITSSILDDNLFISVTAIISYENSS
                     KIE"
     gene            643..>1159
                     /locus_tag="KPH29_003739"
     CDS             643..>1159
                     /locus_tag="KPH29_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1469260.1"
                     /translation="MRITKTTFNDVFSNDANGSIIKVEKYVDKKCASVFEILTYTIIV
                     TNTSRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLDPQQGFYIGRIDASCKKIIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCNLDKFPTRISIESNRVYTNVNKIVFKQLNISN
                     TLKAPKNLRDIL"
BASE COUNT          499 a          105 c          164 g          391 t
ORIGIN      
        1 actattattt atattctttc ctaaaagtaa gtagtaactg aatcgtgaat aatcaaaaat
       61 ggtaatttga aatagttttt atgaatagta ttaatatata gatataggga gattgatgtg
      121 attatgtatg aaaataataa ggtaatatat aattttgaaa gatgtgaaaa gctagaaaat
      181 gctctttatt tatctcaatt taatacatat aaaaacttta agttaacttc taaaaaaact
      241 gtaataaact cattagatac atctacttat aatatgaata ttcatagcat aagtctcgtt
      301 gatactatgg aatgtatatc gtcagaagat gaacaaatga caggaaagaa gttgatttta
      361 gttggcgata tagatactga tgtaacatta gattatgcag gcaataaaag aaatagaaat
      421 tctagtaaaa aaaatacatt taaattaaaa atacccttta gtacatttat acaaatgcct
      481 agaaagatag aaaataaaga taaaattaat ttaaagtatc ttatacaaga tattacttca
      541 tctattttag atgataattt atttattagt gttactgcaa ttataagtta tgaaaatagt
      601 tccaagatag agtaatcttt aaattataga aaggtggatg caatgagaat aacgaaaact
      661 acttttaatg atgtattttc aaatgatgca aatggaagta ttataaaagt agaaaaatat
      721 gttgataaaa aatgtgcatc tgtatttgag attttgacct acactattat tgttacaaat
      781 acaagtaggt acaaaactgg aaatatattt ttcaaagatt atatttctaa atatatagag
      841 tttataaata atactgtaaa agttaatggc attataaaac gtggattaga cccacagcaa
      901 ggtttttaca tagggagaat agatgcaagt tgtaaaaaaa taatatcttt taagagtgta
      961 gttttaccaa attctgcaca taggattata aagaatagtg ctaatatata ctattattat
     1021 aagtgtaatc tagataaatt cccaacaaga atatcaatag aaagtaatag agtttatact
     1081 aatgtaaata aaattgtatt caaacaattg aatataagta atactttaaa agctcctaaa
     1141 aatctaagag atatattaa
//
LOCUS       DAELXX010000248         1155 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.252, whole genome shotgun sequence.
ACCESSION   DAELXX010000248 DAELXX010000000
VERSION     DAELXX010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.252"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(136..627)
                     /locus_tag="KPH29_003740"
     CDS             complement(136..627)
                     /locus_tag="KPH29_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HBG1469261.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDENIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASDDGLAVIF
                     FIM"
BASE COUNT          442 a          163 c           98 g          452 t
ORIGIN      
        1 aaatttacct acagatttaa atttaactta tatattttaa aaggctaata taatatgtat
       61 tttcttctat acaatcaaaa ggcttcatat ctaagatatg ttatgacact aatattcaca
      121 attttttagt aattatcaca ttataaaaaa tattacagct agtccatcat cagatgcttc
      181 ttgtataaac tttttaagct caattagatt tgcccataca tattcaaaaa aatcatcttc
      241 actctcatca ttcataacag gataaatatt attttctaac atctcattaa tattaaatct
      301 ttttctaaat gcttgttctt ccaaactgtt cattgctttt gatagttttt ttacatcatt
      361 tgctgtaatc aacatgacag gaaattctcc atcaatttcc ataagtatgt ttccacttag
      421 tactaatttg ctaaatatat tctcatcatc tccaccaaaa ggacaacctg tcaaagtaaa
      481 gtgaatggca tgccatgctt tatcaatatt gatagtacta ttttcgttgg catcttcaaa
      541 aacaaaatct tgtaaatttt cttctgcttg ctgtaattta aaaacattct cttcacttat
      601 tttagtataa catcctatca tccccataat tacattcctc ctaagtagcg tttttttaat
      661 tgtaacttat aattaaactt agttacaata aaatatattg agaaaagtat tatttcatct
      721 taaatttaaa tacctatatt atcaactaaa attctgtgtt tatagcgtaa gtattattat
      781 tttttcctaa aaattaaatg aatcagaagt tctataggca atattgacta taaataagaa
      841 ctattacttt taaaaaaaat ttaagaaatt atactgtaga atcatgctct acttattagc
      901 ttcaaataaa aaataaatca tgttctcaaa ttaaataata aataacaata aatttaaatt
      961 ttaaactata agagtttctg aagtgttaat aagcatacca ataattatat gtgtaagaat
     1021 ttgatttaat agatatgaat aatttttaca ttaattataa atatattaac ctattattat
     1081 aaattctata aaaagaaaag ttatcttaaa taaaaaataa gtgtatctta aattttaaat
     1141 gatacactta ttttt
//
LOCUS       DAELXX010000249         1085 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.253, whole genome shotgun sequence.
ACCESSION   DAELXX010000249 DAELXX010000000
VERSION     DAELXX010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.253"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..405
                     /locus_tag="KPH29_003741"
     CDS             <1..405
                     /locus_tag="KPH29_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG1469262.1"
                     /translation="RLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNMIKNRRL
                     SRLISDVSWSEFIRQLEYKANWHGRKIVKVGKFFASSQICNKCGYKNEEVKDLNIREW
                     ICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            553..>1085
                     /locus_tag="KPH29_003742"
     CDS             553..>1085
                     /locus_tag="KPH29_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1469263.1"
                     /translation="MFLKLIFKYNTLDFSRAYFDLTDWADNPKDISYYLSNGTFGDLM
                     DSIDENASKMSLSDIAKLEKKIAKKHINLWNEGKIDFSTTRFSSIVPMLQNAGVNFHF
                     IYPDIDLLKNTFNNLIKDIHLHRMKGNQPSVIYITVKDTNYDDSTLDQVYKILNSIKK
                     DKLTDFIIEKNSNSFKI"
BASE COUNT          448 a          121 c          170 g          346 t
ORIGIN      
        1 agacttcaag aacatatagc taatcaaaga aatgattttc tacaaaaatt atctactaaa
       61 ttaataaaag agaatgatat tatttgcata gaagatttac aagtaaagaa tatgattaaa
      121 aatcgtagac tatctcgctt aatttctgat gtatcatggt cagagtttat tcgtcaatta
      181 gaatacaagg ctaattggca tggtagaaaa attgtaaaag taggtaaatt ctttgcaagt
      241 tcacaaatat gcaataaatg tgggtataaa aacgaggaag tgaaggactt aaatataaga
      301 gaatggattt gtcctagttg taatgaaact cacgatagag atataaatgc aagtataaat
      361 atattaaaag aaggattaag actaataaca attcaaaata aataacttat gtaagaaccg
      421 taggaactac ggggatggcc tgtgaagaat tagtaagaca tattttagta tgcaaaattc
      481 tatgaagcag gaaccctgtg actttagtca tgggaggttc agtttggtac tgacttacaa
      541 agttactatg atatgtttct aaagcttatc tttaaatata atacattaga tttttctaga
      601 gcatactttg acttaacaga ctgggcagat aatccaaaag atatatcata ttatttatca
      661 aatggcacat ttggagactt aatggatagt attgatgaaa atgcatctaa aatgagttta
      721 tctgacatag caaaacttga aaaaaagatt gcaaaaaaac atattaatct atggaatgaa
      781 gggaaaatcg atttttctac tactcgtttt agtagtattg ttcctatgtt acaaaatgca
      841 ggagtaaact ttcatttcat ataccctgat atagatttat taaaaaatac ttttaataac
      901 ttaataaaag atattcattt acatagaatg aagggaaatc aaccaagtgt aatatatata
      961 acagttaaag atacaaatta tgatgactct actctagacc aagtttataa aatattaaat
     1021 agtattaaaa aagataaatt aaccgatttt ataattgaaa aaaatagtaa ttcttttaaa
     1081 atttt
//
LOCUS       DAELXX010000250         1008 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.254, whole genome shotgun sequence.
ACCESSION   DAELXX010000250 DAELXX010000000
VERSION     DAELXX010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.254"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(377..844)
                     /locus_tag="KPH29_003743"
     CDS             complement(377..844)
                     /locus_tag="KPH29_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG1469264.1"
                     /translation="MKFLNVLIVVEDIEKSKKFYYDILGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDNFDTFVERLSTMKGIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEIGETMSSVCRRFLDSGLSIDEVAKRMDVTVEYIESVLE"
BASE COUNT          399 a          167 c           61 g          381 t
ORIGIN      
        1 cattatttta tttgaaatat ttattaaata aataaactaa aactgcaatt ttcactatat
       61 ttttaatttg ttcaattaaa ttttaacata attacataaa atagtatata tgattctaca
      121 gcttgatagt aagtataatt atcttttata agctaatact tatatttttc taaatttaaa
      181 tcatccaata aatattaaat tctattataa tatgtaagga aaatcatgca ctttttaaaa
      241 atccaaataa aattataaca aatattatat tctgtatctt acaaaaacaa aaactgtaag
      301 taaatttgtt tccatacaag tttacttaca gcccttactt ctatacaaaa tatagtttta
      361 ctttaatata atccatctat tctaaaactg attcaatata ttcgacagtt acatccattc
      421 ttttagctac ctcatctatt gaaagaccac tatctaaaaa acgtctacac acagatgaca
      481 tagtttctcc aatctcaata atatgcccat ctaaatcata aaatctaatt gctctttgac
      541 cccacctatg ctctatagct agatgaacat aatctatacc tttcattgta ctcaatcttt
      601 caacaaaagt atcaaaatta tcttcctcaa aatataactc agcatcattt ccattgaact
      661 taacttcact gtcactctta ttaataaatt ctaaccaaag ctttttttct tgtaatgata
      721 tattaccttc taatacaaca ttttctccaa aatcacagat tacctttaac cccaaaatat
      781 cataataaaa cttttttgat ttctcaatat cttcaactac tattaaaaca tttaaaaact
      841 tcatattacc tccttaatat acacatactt tatttataat atataattag aattaaacta
      901 taaaggtttc aataaggtct agtataaaga aaaaaattat aagtcttatt gaaatagtta
      961 agttaatttt atcatataat ataaatttta atcttgtaaa aaacagac
//
LOCUS       DAELXX010000251          967 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.255, whole genome shotgun sequence.
ACCESSION   DAELXX010000251 DAELXX010000000
VERSION     DAELXX010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.255"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            161..502
                     /locus_tag="KPH29_003744"
     CDS             161..502
                     /locus_tag="KPH29_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HBG1469265.1"
                     /translation="MKEENFINVEYEDKRFSINSKEVLYIEKQEYEVKIYTESGNYEV
                     GENAPCIRKLLKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLEKLYDYED"
     gene            653..>967
                     /locus_tag="KPH29_003745"
     CDS             653..>967
                     /locus_tag="KPH29_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HBG1469266.1"
                     /translation="MIELKNNLGISERKLKYPCIYKHFKGKYYATMGLSKAIDDIENI
                     CEIYGKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNLYHKKEEDTNDLVLYKTLYD
                     DTG"
BASE COUNT          431 a           66 c          148 g          322 t
ORIGIN      
        1 tttaacttta aaaattaatg ttaaaaaata aacttataca ttaatattaa taataattat
       61 ttaaatatct taagtaaatc agtcttacat atatttccga tagattatat tgataaaaaa
      121 cactaaaatt aaattaataa aagtacttgg agggatggaa atgaaagagg aaaattttat
      181 taatgttgaa tacgaggata aacgtttttc aattaatagt aaagaagttt tgtacataga
      241 aaagcaagag tatgaagtaa agatttatac tgaaagtggt aattatgaag ttggagaaaa
      301 tgctccttgt ataagaaaac tattgaaaga agaaaatttt ttcaaatgca gtaatagtta
      361 tattgtaaat ctagacaagt gtgaatttta tctcagcaat aattgtattt ttgtgaataa
      421 taattttata agaattttag gttcagaaag aaaaaagaaa ttaaaaaaga aattagaaaa
      481 attatatgat tatgaagatt aatattggga ggctgtattt tatacagcct ctttatttat
      541 tgattgtatt attaataaat tttaaattat gataaaatat aaaacaagta acttatttca
      601 gaataaaata ctttttaaaa gaaagggacg agatatggga gattataata aaatgattga
      661 attaaagaat aacttaggta tcagtgaaag aaagttaaaa tatccttgta tctataaaca
      721 ttttaaaggg aaatattatg ctacaatggg tcttagcaag gctattgatg atatagaaaa
      781 catatgtgaa atatatggta aagaaaattt aatccaaaac agaaataaat ataaattagt
      841 tattagacat actgaaagag aagaagatat ctatgtttat agggatttag atggaaacct
      901 ttatcataaa aaagaagaag atactaatga tttagtttta tacaagacct tatatgatga
      961 tacagga
//
LOCUS       DAELXX010000252          914 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.256, whole genome shotgun sequence.
ACCESSION   DAELXX010000252 DAELXX010000000
VERSION     DAELXX010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.256"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            64..729
                     /gene="cwlD"
                     /locus_tag="KPH29_003746"
     CDS             64..729
                     /gene="cwlD"
                     /locus_tag="KPH29_003746"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HBG1469267.1"
                     /translation="MICLVVVSIFEIKNISEDVIKYMPVTNKTIILDAGHGGIDPGAL
                     NKDKSTSEKDINLAITLKLRELIESSGGLVILTREDDSSLYKEENNKTTRQKYNENLK
                     NRKEIISNSNANMFVSIHLNAFEQSKYYGAQTFYPKDKQDSKELSKCIQEELKRVVDK
                     TNNREVKPRDDIYLLKENNIPSVLIECGFLSNEKECKLLTDETYQEKIAWAIYIGIQK
                     YLS"
BASE COUNT          403 a          100 c          147 g          264 t
ORIGIN      
        1 agtatacata tacagggtga tgttgtgaga aagtacataa aacatataat ttttagtttt
       61 gcaatgatat gtctagtagt agtatccata tttgaaatta aaaatatttc tgaagatgtt
      121 atcaagtata tgccagtaac aaacaaaact ataattttag atgctggtca tggaggcatt
      181 gatccaggtg cattaaataa ggataagagt acatctgaaa aggatattaa cctagcaata
      241 acacttaagc ttagagagct tatagaatca agtggtggcc ttgtaatatt aactcgagaa
      301 gatgacagta gcctttataa agaagaaaat aataaaacta caagacaaaa atataatgag
      361 aatttgaaaa atagaaaaga aataatatct aattctaatg ccaatatgtt tgtctctata
      421 catcttaatg catttgaaca atcaaaatac tatggagctc aaacttttta tcctaaagac
      481 aaacaagaca gtaaagaatt atcgaagtgt attcaagaag aacttaaaag agtagtagat
      541 aagacaaaca acagagaagt caaaccaagg gatgatatat atcttttgaa agaaaataat
      601 attccatcag tactgataga atgtggtttt ttatcaaatg aaaaagagtg taaactctta
      661 actgatgaaa catatcaaga aaaaatagca tgggcaatct acataggaat acaaaaatat
      721 ttaagttgaa ataagtataa aaataggtct aaatatgtaa atagcttgta ttaaagcagt
      781 taaaatgcat taatataggc tatttttatt ttgacaaaaa aatatttaaa ataaaagtta
      841 aaaagttgtt gacttagaat aatatagatg atattatata tgagtgccca aaaggagcac
      901 caaaataaga caaa
//
LOCUS       DAELXX010000253          889 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.257, whole genome shotgun sequence.
ACCESSION   DAELXX010000253 DAELXX010000000
VERSION     DAELXX010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.257"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(168..881)
                     /locus_tag="KPH29_003747"
     CDS             complement(168..881)
                     /locus_tag="KPH29_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1469268.1"
                     /translation="MINIIICEGKKSERVILNDYINTFFYNYNFEYNIKEFENGGDLL
                     KKYPNNVDIIFMDIDLNDENGFNVSKEIKKFDESVRIIFVSSSNKYIKKGYEIGVYRY
                     LEKPLKYSLFKEAISSYLKECYKNEAILSIKCKCKIYFIKVDDILYLEVLKNDLTIHT
                     EKGIYTFKNSMKEIESNLEEHNFFRCHKSYLINLMKVNMLDIENNIVFINQNSIIVSR
                     YKIKLLREKLNSCLVDVII"
BASE COUNT          320 a          115 c           65 g          389 t
ORIGIN      
        1 ctttaaaata aaattgttaa tagaattgta aaataaaatc ttaatatatt caatctgtta
       61 ttgatgaaag gtatattttt aactataatt agtttttatt ataaattttt tcatatttta
      121 caatagatat attctattag aatagaatat aagggcaaaa atttacctca aattattaca
      181 tctaccaaac aactatttaa cttttctctc aataatttta tcttataacg acttacaatt
      241 attgaatttt gatttataaa tacaatatta ttttctatat ctagcatatt taccttcatt
      301 aaatttataa ggtagctttt atgacatcta aaaaaattat gttcttctag atttgattct
      361 atttctttca tactattctt aaaagtatat attccttttt cagtatgtat agttaaatca
      421 ttttttaaaa cttctaaata taaaatatca tctaccttta tgaaatatat cttgcattta
      481 cattttatag ataaaatagc ttcatttttg tagcattctt ttaaatatga tgatattgct
      541 tctttaaata gagagtattt taagggcttc tctaaatatc tataaactcc tatctcataa
      601 cctttcttta tgtatttatt tgatgaggat acaaatataa ttcttacact ttcatcaaat
      661 tttttgattt cttttgaaac attaaatcca ttttcatcat ttaagtctat atccataaat
      721 atgatatcaa cattattagg atactttttt aataaatcgc ctccattttc aaactctttg
      781 atattgtatt caaaattgta attataaaaa aaggtattta tataatcatt tagaattacc
      841 ctctctgatt ttttaccttc gcaaataata atattaatca tattacacc
//
LOCUS       DAELXX010000254          859 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.258, whole genome shotgun sequence.
ACCESSION   DAELXX010000254 DAELXX010000000
VERSION     DAELXX010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.258"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT          291 a          100 c           86 g          382 t
ORIGIN      
        1 aaattatata tattataaca attatagcaa atttttaggg tataattttt aaaattatat
       61 tctactatca aaagatatga ataaatgatt gtatatatta tatcaatttt tatatttcat
      121 agtccatttt ttcatcacat tttcgcttgt caagaataat atctttaaga gtgaagcgtt
      181 tgatgtaaat ctggactttg tttttgagac atttttgaaa tatttattaa atccttcgat
      241 tatacttgta atatatatta tagatattac ttattttagt aaaactaaag gctgatgcta
      301 caacattcca gttatttcca aacgttttaa tttctgaagt atactgttta cattattttt
      361 aatctatttt atctaaagtc gataaggctt tttattcatt aactgaccta tatacagatt
      421 taaaatcact tattaattct tttgagtcct tgtatgaaac atatttaaag tatttcttat
      481 ctaatgtatt aaacattttt gtattacaga atgaggataa cttgtagcta ttgtctcttt
      541 gaaataagtc atctgtattt actcctaaaa ctacttatat ctgtagattt tataagttct
      601 ccatcttata taatttcttt gattaattgt tttcttaaat tattcataaa aaaatctccg
      661 ttacgatatt tgattatatt taaatcattg ccataaagaa gacttttatt aactttgaaa
      721 aacaaattat tttacattac tggtgattag ttgctacttt cttttgatat taattctatt
      781 agattttata tagttatata tctctttcta actaagtaat ataatattac tgttatagta
      841 gctactaata ttattgcta
//
LOCUS       DAELXX010000255          850 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.259, whole genome shotgun sequence.
ACCESSION   DAELXX010000255 DAELXX010000000
VERSION     DAELXX010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..850
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.259"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            155..802
                     /locus_tag="KPH29_003748"
     CDS             155..802
                     /locus_tag="KPH29_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1469269.1"
                     /translation="MNILIADDEISMLKILCAYFKKENFNVFTAKNGEEALDIFYENK
                     IDLAILDWMMPIIDGIEVCKEIKENSNTRVLMLTAKSQSEDEIEALNIGADEYIKKPF
                     DPRVLIIRAKKLINYKNTINIKDLKIDFGSSKVFKGDKELLLTKKELELIRCLINNKG
                     MVLSREKLLDLVWGLDYEGDFRTVDTHIRRLRVKIGEDIIKTYRGLGYSLEDFND"
BASE COUNT          359 a           58 c          157 g          276 t
ORIGIN      
        1 tataatcgag atattgttaa gacattgtcg agatattata atatataact aatgtattat
       61 aaatagttgt aaacttcctg taacatatat tttatataat taaagtaatg gatataatat
      121 gaaatataat aatgaatttt agggatgtga ataaatgaac atattaattg ctgatgatga
      181 aattagtatg cttaagatac tttgtgcata ttttaaaaaa gaaaacttca atgtgtttac
      241 tgcaaaaaat ggggaagagg ctttagatat tttttatgaa aataagatag acttagctat
      301 attagactgg atgatgccaa taattgatgg aatagaggtt tgtaaagaga taaaggaaaa
      361 tagtaataca agggtactta tgttgactgc caaaagtcaa agtgaagatg aaatagaagc
      421 actcaacatt ggagcagatg aatatataaa aaaaccattt gatcctagag ttttaataat
      481 aagagctaag aaactaataa attataaaaa tacaattaat attaaagatt taaaaataga
      541 ttttgggtca agtaaagttt ttaaaggtga taaggaatta ttattgacta agaaagaatt
      601 agaattaatt cgttgtttaa tcaataataa aggaatggtt ttatctaggg aaaaactttt
      661 agatttagta tggggattgg attatgaggg agattttaga acagtagata ctcatataag
      721 aagattaagg gtaaaaatag gagaagatat aataaaaact tatagaggat tagggtatag
      781 tctggaggat tttaatgact aaactaaaca aaaaattgtc cataagtata tctatagtag
      841 ttgtagttgt
//
LOCUS       DAELXX010000256          776 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.260, whole genome shotgun sequence.
ACCESSION   DAELXX010000256 DAELXX010000000
VERSION     DAELXX010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.260"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            131..>776
                     /locus_tag="KPH29_003749"
     CDS             131..>776
                     /locus_tag="KPH29_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBG1469270.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILY"
BASE COUNT          353 a           66 c          125 g          232 t
ORIGIN      
        1 atattaaatt ttggaatcaa aatatactag gatataaaat tcttaaataa aaagaaaagg
       61 ttgtttttat aaaaaatatt ttttatacca ctttaaaaaa cacaataata acactgaggg
      121 aggtggggat atgagagaac gcattattct tgaaacatta aaaaatagta gtggttatct
      181 aaacatagat tattttgcag ataagttggg ggtatctact cgaactatca gaaatgaaat
      241 aaaaaagata gaaactataa aagaatgtaa tggttttaaa ttagaatata agtctaaatt
      301 aggatatatt ctaaatatta aagaccaaga taaattcgag cattatctaa gaaacttacc
      361 ctcagattta gttgaaaatc cagaacaacg gttggagtct ataatagtag agcttttagt
      421 aaatgaaggt tataaaacta tagaacagtt aagtaaaaaa tttctagtaa gttcatccca
      481 aataaaaaat gatttaaaaa agatagatga aaagataaag gatacagaat taaaactaga
      541 gcgaaaggct cattatggaa ttaaaataga aggttctatt aaagaaattc aaagtatatt
      601 ggtggatagt tattttagag ggaatagaaa tataacagag tacaggaata agtttataga
      661 taatttaaaa ttagctaata ttagaagtac aataaaaaat gtacttaatg aacatgattt
      721 agaagcaaat ttaacagaac ttgaggaaat attagcacaa attattatat tgtata
//
LOCUS       DAELXX010000257          760 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.261, whole genome shotgun sequence.
ACCESSION   DAELXX010000257 DAELXX010000000
VERSION     DAELXX010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.261"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>760
                     /locus_tag="KPH29_003750"
     rRNA            <1..>760
                     /locus_tag="KPH29_003750"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          204 a          168 c          239 g          149 t
ORIGIN      
        1 caggatggac ccgcgtctga ttagctagtt ggtaaggtaa cggcttacca aggcgacgat
       61 cagtagccga cctgagaggg tgatcggcca cattggaact gagacacggt ccaaactcct
      121 acgggaggca gcagtgggga atattgcaca atgggcgaaa gcctgatgca gcaacgccgc
      181 gtgagtgatg aaggccttcg ggtcgtaaaa ctctgtcctc aaggaagata atgacggtac
      241 ttgaggagga agccccggct aactacgtgc cagcagccgc ggtaatacgt agggggctag
      301 cgttatccgg atttactggg cgtaaagggt gcgtaggcgg tctttcaagt caggagtgaa
      361 aggctacggc tcaaccgtag taagctcttg aaactgggag acttgagtgc aggagaggag
      421 agtggaattc ctagtgtagc ggtgaaatgc gtagatatta ggaggaacac cagttgcgaa
      481 ggcggctctc tggactgtaa ctgacgctga ggcacgaaag cgtggggagc aaacaggatt
      541 agataccctg gtagtccacg ctgtaaacga tgagtactag gtgtcggggg ttaccccctt
      601 cggtgccgca gctaacgcat taagtactcc gcctgggaag tacgctcgca agagtgaaac
      661 tcaaaggaat tgacggggac ccgcacaagt agcggagcat gtggtttaat tcgaagcaac
      721 gcgaagaacc ttacctaagc ttgacatccc aatgacatct
//
LOCUS       DAELXX010000258          700 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.262, whole genome shotgun sequence.
ACCESSION   DAELXX010000258 DAELXX010000000
VERSION     DAELXX010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.262"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..423
                     /locus_tag="KPH29_003751"
     CDS             <1..423
                     /locus_tag="KPH29_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1469271.1"
                     /translation="TISKKYKMRSVNPLKAWVKEYNLHKEIKETPKGGLFMETRKTTL
                     DERVEIVKDCIEHDFNYNLIAKKYFVSYSQVRRWTLKYKENGIVGLEDRRGKAKKEDE
                     LTQLDEIKLELKIVKAEKHKLQLENAFLKKLKNIERGW"
     gene            417..>700
                     /locus_tag="KPH29_003752"
     CDS             417..>700
                     /locus_tag="KPH29_003752"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG1469272.1"
                     /translation="MVITLSKNENKYKAIQSLFNEKKASLMQLCEIAKVNRSGYYKWL
                     NRDKSNLELENIKLATLITNIYEEKKGVFCSLRMTLQLNREYKLNVNHKRV"
BASE COUNT          307 a           78 c          126 g          189 t
ORIGIN      
        1 actatctcaa aaaaatataa aatgaggtct gttaatccac taaaagcttg ggttaaggag
       61 tataatcttc ataaggagat aaaagaaacc ccgaagggag gactctttat ggagactaga
      121 aaaacaacat tagatgaaag ggttgaaatt gttaaagatt gtattgagca tgattttaat
      181 tacaatttaa tagcaaaaaa atactttgtt tcatacagcc aagttagaag atggactctt
      241 aaatataaag aaaatggtat agttggttta gaagaccgaa gaggtaaagc aaaaaaagaa
      301 gatgagttaa ctcaattaga tgaaataaag ttggaattga aaattgtaaa agcagaaaaa
      361 cacaaattac aactggagaa tgcattctta aaaaagttaa aaaacataga aagagggtgg
      421 taattacact atccaagaat gaaaataaat ataaagctat tcaatcctta ttcaatgaaa
      481 aaaaagcttc gcttatgcaa ttatgtgaaa tcgctaaagt aaatcgttct ggatactata
      541 aatggctcaa tcgagataaa agtaatttag aacttgaaaa cattaaatta gcaactttga
      601 taactaatat atatgaagaa aagaagggag ttttttgctc tttaaggatg accttgcagt
      661 taaacagaga gtataaactt aatgtcaatc acaagagagt
//
LOCUS       DAELXX010000259          682 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.263, whole genome shotgun sequence.
ACCESSION   DAELXX010000259 DAELXX010000000
VERSION     DAELXX010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.263"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            275..652
                     /locus_tag="KPH29_003753"
     CDS             275..652
                     /locus_tag="KPH29_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG1469273.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
BASE COUNT          294 a           63 c          102 g          223 t
ORIGIN      
        1 tttaaaaaaa agtattataa tattccttgt gtgaaaatat aggaattttt attaaatagt
       61 tttaatgtgt aaataacatt ctacactatt gtaattaaat atgtaaaaag taaaatattt
      121 aaatagatat attttaaaat ctaactttta atattgtaaa aaaattagaa atttaacaaa
      181 aagtacaaaa attcacattt ttttcataaa gtgtttgact aaaaaattca catgttgtag
      241 aattaaggta gcaggagttt tgagggaggt gtaaataatg ataagaaaaa taccacaagc
      301 agaactaaaa gtaatgaaat tcatttggaa agtagatgtt acagtgacat caaaagatgt
      361 tattgaagct atggaacaaa aatatggctg gaaacaaact actacactga cacttttatc
      421 taggctggta aaaagaggat tcttagatgc tcaaaaaata gacagatata cacattacac
      481 aataagagta aaacacaaag attatttaag ttttgagaca aaggatttcc taagtaatat
      541 acatgatgac tctttgagaa gcttaatttc agcattacat gatgatgaaa atataggtaa
      601 agataagcta gattttctag aaagttattt tgataactta aaggatgaat aattatatgc
      661 tatatgtttt tgaaaatctt at
//
LOCUS       DAELXX010000260          673 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.264, whole genome shotgun sequence.
ACCESSION   DAELXX010000260 DAELXX010000000
VERSION     DAELXX010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.264"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            318..425
                     /locus_tag="KPH29_003754"
     CDS             318..425
                     /locus_tag="KPH29_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG1469274.1"
                     /translation="MGRHLWNPSYFVATVSENTEEQIRKYINNQKKQVK"
     gene            427..>673
                     /locus_tag="KPH29_003755"
     CDS             427..>673
                     /locus_tag="KPH29_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG1469275.1"
                     /translation="MVVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KNNKETFTYKQCSSDLNNLKKELKWLKEPDKFSLQNAL"
BASE COUNT          275 a           70 c          126 g          202 t
ORIGIN      
        1 tattggtagt ggaagtgggt agaccttaac ggataatgcc gaaggataag atatagtctg
       61 tgcttgcatg aaagtgtaag aagttcataa gagaactgca taggtagtag cgaacttatg
      121 tgaacgacaa cctcaaaaat gataaaagtt aagacttatg gttcttatat atgttattta
      181 aaattaaata taaaaaacct tcaaaatact tgttttaagt aattgcatat tttaatatta
      241 tgataggtga tgaaaaatgg aagttactca tggtagagga tatgcatata gtatgtcaaa
      301 tatagacagc cagcattatg gggagacatc tttggaatcc tagttatttc gttgctactg
      361 tatcagaaaa tacagaggag cagataagaa aatatattaa taatcagaaa aaacaagtaa
      421 agtgaggtgg tagtagtgga aaaagcttat aagtttagaa tgtatccaaa taaaaagcaa
      481 caagagttaa ttaacaaaac ttttggatgt tgtagatttg tatataataa gtatcttgct
      541 aaaagaatag atgtttataa aaacaataaa gaaactttta cttataaaca gtgtagttct
      601 gatttgaaca atttaaaaaa agaattaaaa tggcttaagg aacctgacaa attttcacta
      661 caaaacgcct taa
//
LOCUS       DAELXX010000261          586 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.265, whole genome shotgun sequence.
ACCESSION   DAELXX010000261 DAELXX010000000
VERSION     DAELXX010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.265"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(97..576)
                     /locus_tag="KPH29_003756"
     CDS             complement(97..576)
                     /locus_tag="KPH29_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBG1469276.1"
                     /translation="MPNKLIKNLLSGILQILFFLLGLVIVVGGFKSFMYLCFSGEATL
                     QGTISGILMFILGVSYFIIIKSLIEVLSSSEHSLFVKDNVKRFRIIGYLLLLNSIMEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
BASE COUNT          232 a          100 c           48 g          206 t
ORIGIN      
        1 atagtttatg tattataccc taatatattt tattttaaat tactagttaa tcactctttt
       61 ctggttattt atctcctata tattaaagtg tcacacctaa atagttaaat cattatcctc
      121 ttttatcttt attgccttta caaatccatc tgcaattaca aaactcataa gagaagttat
      181 aaaatataca aatactggaa cagtaaaata aaatccaaat cctaaatcta ggaatctcat
      241 tccttttcca gtagtaccaa aagttgaaat aaactccatt atagagttta aaagcaaaag
      301 atatccaata atcctaaatc tttttacatt atcttttaca aataagctat gttcagaact
      361 acttaatact tctatcaatg attttataat gataaaatat gatacaccta aaataaacat
      421 taatattcca gaaatagtgc cttgtagagt tgcttctcca ctaaaacaga ggtacataaa
      481 acttttaaat cctcccacaa caattactaa ccctaaaaga aaaaataaaa tttgtaatat
      541 accacttagt aaatttttta taagtttatt aggcatcata ccaact
//
LOCUS       DAELXX010000262          556 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.266, whole genome shotgun sequence.
ACCESSION   DAELXX010000262 DAELXX010000000
VERSION     DAELXX010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.266"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            294..>556
                     /locus_tag="KPH29_003757"
     CDS             294..>556
                     /locus_tag="KPH29_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG1469277.1"
                     /translation="MNLQSKWVNFLKPTYVSDEIKRKKLQVIIFFSITYGLSYSLGLL
                     AICFNHLIDSEILAQFMMILPLSSVSIAKFYTEGRNNDKYNFYS"
BASE COUNT          228 a           43 c           69 g          216 t
ORIGIN      
        1 atttgttgta tttttttgta tataaaaata tattgtaata ttaaaaaaat aatagtaaac
       61 tgattgcata aaaatgattt ttatataaat tttaaacaga agaataaatt tctaacgaaa
      121 ttggatattt cttctgtaaa tacatattca aaaaaagaat tataagagtt tagtcagcag
      181 tcgtgtaagt aataaggact ttataaatat atagaaatat aatgatatga gtataagtag
      241 aaaaatttta tgatttttac aattattttt tataaaaagg gaggttatgt ttgttgaatt
      301 tacaatcaaa atgggtaaac tttttaaagc caacatatgt cagtgatgaa ataaaaagaa
      361 aaaaactaca agttataata tttttttcta taacttatgg tttatcttat tcactaggtt
      421 tattagcaat atgttttaat catttaatag attcagaaat tttagctcaa tttatgatga
      481 tattgccatt atcttcagtt tctattgcaa aattttacac agaaggaaga aataatgata
      541 aatataattt ctattc
//
LOCUS       DAELXX010000263          522 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.267, whole genome shotgun sequence.
ACCESSION   DAELXX010000263 DAELXX010000000
VERSION     DAELXX010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.267"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT          229 a           50 c           57 g          186 t
ORIGIN      
        1 agacagtttt acatatttca actgtctcct aagaggggta tagttcacaa ctaagagtgg
       61 ctgggaactt aatataaagt tccagaaaag taaatcttca aaataattta ttgaaggaaa
      121 catcactcaa agttaagtaa aatatgtttc cttgctttta ttatatcaca aattaaaaat
      181 attaacacaa aattttgtga atatatactt attattgttc taaaaaaata tgtaaataat
      241 tgatttgatt tcatagaatt attgttgtaa tatttagtta aatatacaca atctcaatta
      301 aagaagataa gtattataaa ttcatatcaa aaaaatacat aaacttttaa gttataattt
      361 tataaaaagt cttttagtat cattatttca agaggaatta acatatgaaa aaataacttc
      421 attagaaaag tggataaaaa actggtaaga agatgactaa agattacata tactattact
      481 taaattaaaa atttaatata atttataatg tatacaattt at
//
LOCUS       DAELXX010000264          514 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.268, whole genome shotgun sequence.
ACCESSION   DAELXX010000264 DAELXX010000000
VERSION     DAELXX010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.268"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>514
                     /locus_tag="KPH29_003758"
     CDS             <1..>514
                     /locus_tag="KPH29_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG1469278.1"
                     /translation="TSLYIFKNFKIGILLVFYFLFSQIIFGLIISDNQFDYKNFLNYI
                     LLPIISLVYIYPFLCEEYGWRSFLQSILFDRFGKKIGIVMVGTCWSLWHLPLQFTLYS
                     PQTPIIGAIAHLIYGIGLSIFLGYVYMKTKNIWFCSIMHVLINSLGLIFNDSEIIINY
                     HSIIQRLIFIL"
BASE COUNT          168 a           51 c           69 g          226 t
ORIGIN      
        1 aacaagttta tatattttta aaaattttaa gataggtatt ttgctagttt tttacttttt
       61 attttctcaa attatttttg gattaataat aagtgataat caatttgatt ataagaattt
      121 cttgaattat attcttcttc ctataatatc attagtatat atttatcctt ttttatgtga
      181 agagtatggt tggagatctt ttttacaaag tattttattt gatagatttg gcaaaaaaat
      241 aggtattgta atggtaggca cttgttggag tttatggcac ttacctttac aatttacact
      301 atatagtcct caaactccaa taattggagc tatagcccat ttgatatatg gtattggact
      361 ttctatattt ttaggttatg tttatatgaa aactaagaac atatggtttt gctctataat
      421 gcatgttcta attaatagct taggattaat tttcaatgat tctgaaataa ttattaatta
      481 ccatagtatt atacaaagat tgatatttat acta
//
LOCUS       DAELXX010000265          420 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.270, whole genome shotgun sequence.
ACCESSION   DAELXX010000265 DAELXX010000000
VERSION     DAELXX010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.270"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>420
                     /locus_tag="KPH29_003759"
     rRNA            <1..>420
                     /locus_tag="KPH29_003759"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          106 a           97 c          130 g           87 t
ORIGIN      
        1 cgcctcccaa aaggtaacgg aggcgctcaa aggttctctc agtacggtcg gaaatcgtac
       61 gtagagtgta aaggcaaaag agagcttgat tgcaagacat acaggtcgag caaggatgaa
      121 aatcggactt agtgatccgg tggttctgcg tggaagggcc atcgctcaac ggataaaagc
      181 taccctgggg ataacaggct tatctccccc aagagtccac atcgacgggg aggtttggca
      241 cctcgatgtc ggctcatcac atcctggggc tgtagtaggt cccaagggtt gggctgttcg
      301 cccattaaag tggtacgcga gctgggttca gaacgtcgtg agacagttcg gtccctatcc
      361 gtcgcaggcg taggaaattt gagaagacct gtccttagta cgagaggacc gggatggacg
//
LOCUS       DAELXX010000266          407 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.271, whole genome shotgun sequence.
ACCESSION   DAELXX010000266 DAELXX010000000
VERSION     DAELXX010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.271"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT          130 a           59 c           28 g          190 t
ORIGIN      
        1 taattcaatt taaattttaa atattttgtg tttattatat aatctgaaaa aaaagcagtc
       61 aatagcattt ttaatttttt taattttcaa aattatgatt cttaaaaaat cgttttcctt
      121 ttatcataac acctattaac gttaattctt gtagtatttt gtatttattt tgctaaattg
      181 acagttaact ttattcaatt tttattctac tcacgtagat ttacctcttg tcttttagtc
      241 ttaaaaccat tttttctgaa aattccttat tatgatttat caacttattt ttcttcatag
      301 caattacttt ctaacttcca acttacttaa actttgcagt tgctagtatt cacgtttaca
      361 tacttatatg tcataattaa ctaaaaattc ttaatatatc tctcaat
//
LOCUS       DAELXX010000267          389 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.272, whole genome shotgun sequence.
ACCESSION   DAELXX010000267 DAELXX010000000
VERSION     DAELXX010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.272"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     regulatory      142..232
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          162 a           39 c           56 g          132 t
ORIGIN      
        1 tcaaagagtt tattataaaa ttgtagacta tagtgtttat aatctttgta attattggta
       61 actaaattta aaaaataaaa tatttaatta tacacaaaaa agatatttaa aaaatttgtt
      121 atttattgta tacttaagtt aaaactaaat aggcaaatct agagaaatct agtgacgcaa
      181 agctataggg gctaaggttt gtatacacaa actatgtcag ccagttgcca aaaagagttc
      241 taggtatatt gtatacgtag gaagagtcta ggatgactgc ctttttggtg gtcattttgt
      301 tatggaaaaa atcacattat gtagtaaaaa acacctataa taacatatta taccaaatat
      361 aatttaaaat aaagataaat tatctataa
//
LOCUS       DAELXX010000268          373 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.273, whole genome shotgun sequence.
ACCESSION   DAELXX010000268 DAELXX010000000
VERSION     DAELXX010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.273"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            177..293
                     /gene="rrf"
                     /locus_tag="KPH29_003760"
     rRNA            177..293
                     /gene="rrf"
                     /locus_tag="KPH29_003760"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            300..371
                     /locus_tag="KPH29_003761"
     tRNA            300..371
                     /locus_tag="KPH29_003761"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:332..334,aa:Asn,seq:gtt)
BASE COUNT          118 a           61 c           80 g          114 t
ORIGIN      
        1 tttgatgtta ttcattaaga tatatgtgca gtttttagag tgttaactct atgtaaagta
       61 ctaacttcaa ttgatgaagt atatatcgta aaaacaatta ctatttagta cttcaaaact
      121 attgacattt aaaaataaag atgttaatat aatacttgtc ttataaatag gcaatatgtg
      181 gttataatag caaagaggat acacctgttc ccattccgaa cacagaagtt aagctcttta
      241 gcgctgatgg tacttggtgg gaaactgcct gggagagtag gacgtagcca cgtgaatatt
      301 ccaaggtagc tcaatggtgg agcaaccggc tgttaaccgg taggctgtgg gttcgagccc
      361 cacccttgga gcc
//
LOCUS       DAELXX010000269          356 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.275, whole genome shotgun sequence.
ACCESSION   DAELXX010000269 DAELXX010000000
VERSION     DAELXX010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.275"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT          158 a           16 c           48 g          134 t
ORIGIN      
        1 ttatgatagt atagataaag tttattcttt ttattaatag aaattgttgt ataaaaataa
       61 taaaagtgag gagagtgata tttaaataaa aaaattttaa taagtattgg taatatagtt
      121 tttagggatg attattttat gtaaaaagtt gttatttgta aagataaata tagaaaatat
      181 tataagtatt gatttagtga taatctaaaa ggtgagaatt gtaataaaaa gagaacatat
      241 ccttcaaatg gatgtaaact tataaataac attattacaa gagatttatt atacatatac
      301 tgcaactatt aattagaatt tatactgtat ttgtatttaa ataaaaacac aaataa
//
LOCUS       DAELXX010000270          343 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.277, whole genome shotgun sequence.
ACCESSION   DAELXX010000270 DAELXX010000000
VERSION     DAELXX010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.277"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>343
                     /locus_tag="KPH29_003762"
     rRNA            <1..>343
                     /locus_tag="KPH29_003762"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           81 a           76 c           98 g           88 t
ORIGIN      
        1 tgcatggttg tcgtcagctc gtgtcgtgag atgttgggtt aagtcccgca acgagcgcaa
       61 cccttgtctt tagttgccat cattaagttg ggcactctag agagactgcc agggataacc
      121 tggaggaagg tggggatgac gtcaaatcat catgcccctt atgcttaggg ctacacacgt
      181 gctacaatgg gtagtacaga gggttgccaa gccgtaaggt ggagctaatc ccttaaagct
      241 actctcagtt cggattgtag gctgaaactc gcctacatga agctggagtt actagtaatc
      301 gcagatcaga atgctgcggt gaatgcgttc ccgggtcttg tac
//
LOCUS       DAELXX010000271          305 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.279, whole genome shotgun sequence.
ACCESSION   DAELXX010000271 DAELXX010000000
VERSION     DAELXX010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.279"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT          135 a           21 c           36 g          113 t
ORIGIN      
        1 ataaaagata taagatgcct tatatttatt tttgcatata agtatagtct tcgtaataat
       61 tagtatacca tagtgattta tttttaattg tgtatttata taaatacaca acttatgata
      121 tggacgaatt attatgcaaa taagctgtaa ttaaataaat tgcaatataa accagttgaa
      181 acataagtta gggaaatatt atcaaagtta ttatttagca taagagtata gagggaataa
      241 aataagatta aaaaattaca tactaaattt aataaaatta ttatattaaa aataatatta
      301 agttt
//
LOCUS       DAELXX010000272          295 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.denovo.280, whole genome shotgun sequence.
ACCESSION   DAELXX010000272 DAELXX010000000
VERSION     DAELXX010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.denovo.280"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT          127 a           28 c           32 g          108 t
ORIGIN      
        1 ttgaattgtc attaaatatg ccaatagttt tatatattat tgattttatt aaaaaaagag
       61 atatacaaac taataagttg taatctctca aatataaaaa aacaataaat taactcattg
      121 gattttttag tccataatat aattatagat tattcaacaa acaatctata gaaattgtgg
      181 tattaatgtt taaataacca actttttcta aaataacctt tataggaata tatgagttca
      241 taagtaaaga tagcgaaata agaaaccagt ttggtatgta aaatctagat tttta
//
LOCUS       DAELXX010000273        12107 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.guided.111, whole genome shotgun sequence.
ACCESSION   DAELXX010000273 DAELXX010000000
VERSION     DAELXX010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12107
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.guided.111"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            34..735
                     /gene="vanR"
                     /locus_tag="KPH29_003763"
     CDS             34..735
                     /gene="vanR"
                     /locus_tag="KPH29_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HBG1469279.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KDLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            785..1867
                     /locus_tag="KPH29_003764"
     CDS             785..1867
                     /locus_tag="KPH29_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG1469280.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            2128..3228
                     /gene="vanG"
                     /locus_tag="KPH29_003765"
     CDS             2128..3228
                     /gene="vanG"
                     /locus_tag="KPH29_003765"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HBG1469281.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            3225..4031
                     /locus_tag="KPH29_003766"
     CDS             3225..4031
                     /locus_tag="KPH29_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG1469282.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            4049..6187
                     /gene="vanT"
                     /locus_tag="KPH29_003767"
     CDS             4049..6187
                     /gene="vanT"
                     /locus_tag="KPH29_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBG1469283.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            6501..7121
                     /locus_tag="KPH29_003768"
     CDS             6501..7121
                     /locus_tag="KPH29_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBG1469284.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(7287..7823)
                     /locus_tag="KPH29_003769"
     CDS             complement(7287..7823)
                     /locus_tag="KPH29_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBG1469285.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(7936..8610)
                     /locus_tag="KPH29_003770"
     CDS             complement(7936..8610)
                     /locus_tag="KPH29_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBG1469286.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            8813..10687
                     /gene="asnB"
                     /locus_tag="KPH29_003771"
     CDS             8813..10687
                     /gene="asnB"
                     /locus_tag="KPH29_003771"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBG1469287.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEYVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            complement(10779..>12107)
                     /locus_tag="KPH29_003772"
     CDS             complement(10779..>12107)
                     /locus_tag="KPH29_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBG1469288.1"
                     /translation="KEVHFTRYIGVDDSIDKYIEKVKNIDTYFFNNPTIPYIRLNNLK
                     INFDKEQTDKMLKIFNIYDSSKFNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFS
                     TSMPNCTSSIIKNFAVKLLCWMDYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYF
                     LSQLGCDILYINPNKDILDLYPECSKFSTLFELSRKTPNILEIPFERHITNINYSENT
                     NKTSPTTNKPNSKNTLNNTNLTSKKIAQDEEVELSYEDLAKLSASVVMIVVCNNENKP
                     FKSGSGVVINNEGYILTNLHVVNDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKV
                     DRKCKPIPVKVSKKPVRGQKIVAIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPIS
                     SGSSGGALLDMFGNLLGLISAGYDDGQNLNLAVESNLVKIFANNFIEIVN"
BASE COUNT         4543 a         1392 c         1954 g         4218 t
ORIGIN      
        1 gacctcgata ataaggagag tgaaaaataa tctatgaacg aaaatatttt gattgtggat
       61 gatgaaaaag aaatagcaga tttaattgaa gtatatttaa aaaatgaagg ttttacagta
      121 tacaagtttt atacaggtaa ggaagctcta gaatgtattg aatctaaaga tttggatatg
      181 gctattttag atattatgct tcctgatatt gatggatttc atatttgcca gaaaattcga
      241 gaaaaatact attatcctat cattatgtta acagctaaaa ttgaagatat agacaagata
      301 atgggtttaa ccataggtgc tgatgattat ataacaaagc catttaaccc tttggaagtt
      361 gttgcaagag taaagacaca attaagaaga tatatgcgat ataataattc ttatgaacaa
      421 cagtcgataa ttgttaatga gtatgatatt aaaggattga ttatcaataa agaaacacat
      481 aaatgtagtt tatttgggaa agaagtagca ttaacaccga ttgaattttc tattctttgg
      541 tatttatgtg aacatcaagg aaaagtggtt ccttctgaag aattgtttga agctgtatgg
      601 ggagaaaaat atcttgataa taataacaca gttatggctc atatagggcg attacgtgaa
      661 aaaattcatg aaccttctaa aaacccgaaa tttataaaaa ctgtatgggg agtaggttat
      721 acaattgaaa agtaaaaata ttaaagtcaa tcctgacttt actcaagtga gaaataaaat
      781 ttttatgaga acagctttaa tggcatttat agccattatt ttcatatgga ttttatattc
      841 ttttatattg tatggtaagt tttctatctg gatagtatca ttctttgaaa atgcttttcg
      901 tatggaatat aaagctgcat tgaatttata tcagcagatt ttccgtaata gcatggagct
      961 cttcatgata ctagctatgg tgattgtgtt ttttataata ttccgtgttt atttaaattg
     1021 gtttacaaaa tattttagtg aaataaatca aggtattgac tctcttatca aagaagatgt
     1081 tggagaagtg gctttatctc cagagttatt ggcaatagaa aagaaaataa actcaattaa
     1141 acatatactt gaacaacgta aatttgagac acaaatggca gaacaacgta agaatgaact
     1201 tattgtttat cttgcacatg atttaaaaac accacttaca tcagttatag ggtatctgac
     1261 actacttcga gatgaaagtc agatttctga agaactgcga aaaaaatatt tatctatttc
     1321 gttggataag gcagagcgtc ttgaagattt aattaatgaa ttctttgaaa ttacaaggtt
     1381 taatttatca aatatatcac ttgaatacag tacagtaagc ttaactcgta tgcttgaaca
     1441 actaacatat gagtttaaac ctactttgat gagtaagaat ttaaaatata cactgaatgt
     1501 tacaccaaat atgatgataa ggtgtgatgt taataaaatg caacgtgtat ttgataactt
     1561 attacgaaat gctgtttatt atagttttga agatactact attgaaataa cagctgtaca
     1621 ggaggatgat tatataaagc ttaaatttat aaatcatagc aatacgattc cagaggaaaa
     1681 acttgagcgt atttttgaac aattttacag gttggataca tctcgtaatt caaacagtgg
     1741 tagagctggg ttgggacttg ctatagctaa agaaatagtg gagcttcata acgggacaat
     1801 aacagctcat agcgagaaca atataattac ctttaaggtt attattccga ttgtaggaaa
     1861 tttgtaagaa tttaagtata aaaaaatagg aaattcttta ggataaataa ataaagagca
     1921 ctcttgttat tgatataatt actgtatcaa aacaagagtg cttttatttt gatacagtaa
     1981 ttatatcaat agaaaaatgt gaaaatatat tatgtggaac agatgcaatt gtaagtgaag
     2041 gttgattgga tttgaaatag aaaaagtatt ttaacatgtt tctgtagatt gtataaaaat
     2101 aaataaatca acaaaggaga atcaaagatg aataataaaa aaattgcagt tatatttggt
     2161 ggaaactcaa cagaatatga tgtatcatta caatcagctt ttgcagtact tgagaatttg
     2221 aatacggaaa aatatgatat tataccaatt ggaattacaa gggagggtaa ttggtatcat
     2281 tatattggag attataataa tatccagaat aacacttggt ttgaaaggga tactgatata
     2341 attccagtta ttgtttcgca gaaccgtgtc aaaaatggta ttattgaatt tttaattcct
     2401 gatgctgttt ctcagaatca tattccaaat agtgctattg aatcatgtga aaatgatatt
     2461 aaatacacta aacttgattt tgtatttcct gtattgcatg gaaaaaatgg agaagatgga
     2521 acagtgcagg ggttatttga attggcaggt atacaggtta ttggatgtgg tactctttct
     2581 tcagcattat gtatggacaa agacaaatca cataaacttg ttcattcaga aggtattaaa
     2641 gtaccaaaat caatattact aaaatactca gataaattta atgtggctga actgtcaaaa
     2701 gacttaactt atccattatt tataaaacct gtgcgtgcag gttcatcatt tggtattagt
     2761 aaaatttata aaaaagaaga gttacaagta gcagtagact tggcttttga acatgataat
     2821 gaagtaataa ttgaggaaaa tattgatggt tttgaggtag ggtgtgcagt tttaggaaac
     2881 gatgacttac ttgttggaag agtagatgaa atagaattgt caaaaggatt ttttgattat
     2941 acagaaaaat atacattgaa aagttcaaaa attcatatgc cagctagaat tgatttggat
     3001 tgtgagaaaa gaatacaaga gacagcaaaa attatttata gaactcttgg atgttctgga
     3061 tttgctagag tagatatgtt tttaactcca tctggtgaaa ttatttttaa tgaagtcaat
     3121 acaattcctg gctttacttc tcatagtcgt tatccgaata tgatgaaagg tattggactg
     3181 tcttttgaag aaatattgga taagataatt gaggtgtaca taaaatgatg aataatattt
     3241 tattagataa aaataaagaa gataaaatga caaatactat cttattgact aaagataaag
     3301 tttatgatgg gaatctaatt ctagtaaatg cttttttacc agttaaaaca tctgaagata
     3361 tagatttaat tcctgtagat acaaggtttc caagtattct tatgaaacgt gaagctacaa
     3421 atattttaca aaatatttta aaaagtatat gtggaataaa tgaaatagta cctgtaagtg
     3481 gatatcgtac tgcagaggaa caacaagata tatattcaag ctctctgcgt gacaatggaa
     3541 aagactttac taaaaagttt gtagcacttc caaatcacag tgaacatcag acagggcttg
     3601 ctattgattt agggttgaag aaagaggtta tagattttat ctgccctgaa tttccttata
     3661 atggtatttg taatgagttc cgtaaggcag cactacatta tggttttata gagcgttatc
     3721 agtgtaaaaa agaagaaata acaggaatat ctcaagaacc atggcatttt cgttatgtcg
     3781 gatatccaca ttctgagatt atcaataaaa tgaatctcgc ccttgaagaa tatatagaat
     3841 atattaagag attttcgtat acaaattgtt taaaaataga agataaagaa aaaattatag
     3901 aaatctttta tgtgcctatt catactaaag aagaagtgag aatctctata cctgaacaat
     3961 gtgtatatca aatatcgggt aacaataagg atggttttat tgtgacattt tggagaaata
     4021 gaaaggacta agaaatggag gaacaagaat gattaggaat aaatcttatg cagggcttga
     4081 ttattttagg attatagctg cattacttat aattgcaatt catacatctc cattattatc
     4141 atatagtaaa atggctgact ttgtgttaac tagtataatt gctcgtattg ctgtgccatt
     4201 cttttttatg ctatcaggct tttttctaat ttcaacatat aattataatt gtaataaact
     4261 gaaatctttt ctaaaaaatg tattatttat ttacgggata gctattgtaa tttatattcc
     4321 cttaaatatt tacactggat atttttccat gaataattta gtgcccaata ttattaagga
     4381 cattgtattt gatgggactt tatatcattt atggtatcta cctgcatcaa ttattggtgc
     4441 aagtattgca tactttcttg taaaaaaact aggtttaaag tactcattag taatcactat
     4501 tgttatttat gtaattggta tgtttggtga tagttattat ggttttttag aaaaaatacc
     4561 actattaaag aatttatatg ggtatatttt tgaagtatca gattatacac gaaatggtat
     4621 attttttgca cctgtatttt tcatacttgg tggaataata tctaataaat ctaattatat
     4681 gtcaactaaa aatagcttga ttggatttct tatatcattc ttacttatgt tatgtgaggg
     4741 aatgttttta cataaactag gggtacagcg tcatgatagc atgtatatta tgttattgcc
     4801 atgtatgtat ttcttattta cagccttaac atttttaaaa ggttctcgag ctaggttatt
     4861 gcgaacatca tcattaatta tttatattat acatcctatg atgattgttg ttattcgtat
     4921 gttttctaaa atattcggaa tacaaactgt cttggttgac aatagcctta ttcattatat
     4981 agtggtaagt atagcatctt ttatagcttc tataattatt atattgattc ttaaacctct
     5041 aaaaaaagac ttgaaaggag aacataaggt aaataccaat agagcttgga ttgaaattga
     5101 tttaaataat ttggaacata atgtaaagat gatacaaaaa gctatgccag aagattgtga
     5161 attgatggca gttgtaaaag ctaatgccta tggtcatagt gcttttgaag tagcgacttg
     5221 tgcaaaccaa ataggtgtaa aagcatttgc agtagcaaca atagatgagg ggattgattt
     5281 acgtcgatat ggaatactag gagaaatatt gattcttgga tatactaacc caataagggc
     5341 aaaggaactt cataaataca atcttacaca gagtgttatt gaccttaatc atgctatctc
     5401 tttaaacaaa cagaaataca acataaaatc acacattaaa attgatacag gtatgcacag
     5461 actgggtttt gatgttaatg atgtgaggtc aattttaaag gcatttaaat taaaatactt
     5521 agatatatgt ggcatattta cacacttgtg tgtttcagat agtctgaaag atgaagatgt
     5581 agactttaca aataaacaaa ttgaatgttt ttataagtta attgattttc tgttaaaaaa
     5641 aggtataaaa attcctaaag tacatattca aagtagttat ggttttttta attatcccaa
     5701 tcttaagtgt aattatgtaa gaattggaat tgcattatac gggatattaa gttctccaaa
     5761 tgatacgact aaattgcaac ttgatttacg acctgtttta tccttaaaat cacaagtaat
     5821 tcttattaga aaaatacaaa aagatgatag ttttggatat ggaagagtct ttattgcaag
     5881 cagagatagc gtaattgcta tattatctat tggctatgcc gatggtatac ctagaaattt
     5941 atcctgtgga aaaagttacg taataataaa tggttgtaga gcagcaatag tgggtcgaat
     6001 ttgcatggac caacttgctg ttgatattac agatatacct aatgtagaag ttggtaatac
     6061 ggcaataata ataggaagag ataatttatc tgaattatct gcttctgaag ttgcaaataa
     6121 ctcatgtagt atatcgaatg aattattgag cagagtagga agaaggttaa atgttataaa
     6181 gaagtaactc aattaaatag taatatttgt tgtactgtgt aatttatttt actctgacta
     6241 gacattttta gtaatattat gtctaatata tattttttat aacttacatg acaatttaag
     6301 ttataaaaag gtgatgtttc agatttttat tttgaaacat cacctttttt gttataatat
     6361 ataataaaat aattatacct taactgtaaa cttatataga ttatttactt tttaatatta
     6421 gattcatttt ttgagattgg tatataataa acatatagag tatataataa tagttaaagt
     6481 taaaaaaggg gaaaagcaat atgcagtata tacaagaatt acttgatttg actcaaaatt
     6541 attgggtgtt agccacaata atcggcttat taagtgcatt tatagaaagc tttataccag
     6601 ccttaccact tgttgctata gttacagcaa atgctgcaat acaaggatta tttattggat
     6661 gtttgttatc atggattggc tcaggacttg ggacaacttc attattctta ttaataagca
     6721 gatttaatga tagtaagctt tttaataagt tgagaaatag taaaactgaa aaagcaataa
     6781 gttggatgga taaacaggga tttaagttat tatttatagc gtatgcctgt ccatttatgc
     6841 ctggttgttt agttactatc gcatcagctt ttggtaaaaa agatttaaaa gattttgtgc
     6901 cagcaatgtt ggcaggaaaa tttgtaatgt ttatagttat aagttacgtg gcaagtgata
     6961 tagaaggttt tataacaagt cctttaaaga tagcatcatt tatattattg gtgtttttgt
     7021 catggaagat tggaaataga gtaaataaaa atcttgaaaa tcataattat gatttccatc
     7081 ataaaaaaca tcacaatgat aaagatgata aaatgattta gcttgtacga aataaaacgt
     7141 atcttaaaat gatttagtac taaattattt taagatacgt tttatttttt gtattaaggt
     7201 acaaaatttt tagttctttt gtaaactaaa atatataaaa cttatttata cttagttcaa
     7261 aatattgtta tttatttttt ctacaactat aataaaatat aaaattgata tccaatcaaa
     7321 aaaataacta tgaaatttaa tagcttagat atgaattttt ctgtttttga gttagttttt
     7381 ataggtagta aagaaatgtt tatggtaatg ggataaaaca attcaatacc ttctttagta
     7441 ataaggtcaa gacacaagtg agatagatat cctattataa acccactgga caaacatgag
     7501 aaaactatat tattgttagt gaagattgtc agagatttaa aaaaactata aataagtaat
     7561 aaggcaatta gactgtgagt aaaccctcta tgtctaaacc tacttccaaa taatttgtat
     7621 attaacataa attttttact tatgtaagaa cctctcatat ctatatctgg aaataaagaa
     7681 cctaaatatg caaaataaat ataaattata attaatacta ttttatatgc aatatcgtat
     7741 ttaactaggt acagattgtt tataaagggg agtatcaaaa gtgcgaatat ataacctccc
     7801 ctttcatgtg tttcttttat catataaaaa tcctcctgaa tcgtcactat aaacattata
     7861 ttggtaaatt tagactatct tttattatat tatatagcaa acataagttt ttttcaaaag
     7921 gattttaata tttaattaat cttgagattt taaatttttg taattttcta aagcaccatt
     7981 ccaatttact acattaaacc agttgtcaat gtattcattt cttctatttt gatattttaa
     8041 atagtaagca tgctcccaaa catcaagtcc tattatagga gttaggtttt tacttacagg
     8101 gctatcctga tttggagtag tcataataga taatttccca tctttagtag ctacaagcca
     8161 agcccaacca gaaccaaaga catctaaagc agatttttgg aactcttgct taaatttttc
     8221 aaaagaacca aagtctctat caatagcttc ttttaaagat tcagaaggta tggttttttc
     8281 tggcgtcatt atatcaaaaa agaatttatg attataagct ccacctgcat tatttcttac
     8341 agttgtagca atatctttag gtaaagaatc taaattttgc aataattcac ataaagaata
     8401 attataaagc tcaggatatt tttcaagagc agcatttaat ttatcaacat aagcttgata
     8461 atgcttatca tgatgcagtt tcattgtttc tttatctata taaggttcaa gtgcatcata
     8521 tgcataaggt aatggtttaa ccttaaattt gttattttca ggtgtaaaag caaatgaagt
     8581 tatgcactgt gagattataa ataaagacat aataactggt attaatattt ttttcttcat
     8641 aaaaatcacc tcctaatata gtatttctag aaataaaaaa aatatgaaat attaacagat
     8701 aatttataag aatttaatag caatttcatt ttaaattgaa taagttttaa agccaaagac
     8761 aaaactatga aatatagaat aagactaaag gacagaaagg aattttgatt atatggacgg
     8821 caatatttca atttattata aaggtgatat atctaaaaaa gaagatatta aatttaaaat
     8881 aaaagaatct gtaaataata caagactttc tagtagaata gagtataagg atgaggaaaa
     8941 aacttttgat tttgaaagat ttaatgtaat tgatgctatt tatggaaata aaccatttga
     9001 aaaagatgga aaagttattg tttttaatgg aaatatatat aactatcatg aaataaaaga
     9061 agaattaatt aaaaaaggac acaattttac tacaaatgat gatattgaag tcttattggc
     9121 atcttatata gaatttggta agaattgtgt acataagatt aaaggaatgt tcaactttat
     9181 aatctatgat agagaaaatg aaagtatatt tggagcaaga gatttatttg ggataaaacc
     9241 actttattat ataaataagg aaaatgcaat aattttttcc tcagagtata agtttttact
     9301 tgaatatatg aaaaatttaa atataaatga aagaagctta caaagttatt tttcatttca
     9361 atatgtactt cctgaagata ctatgataca aggaataagg ctgataccag caggtcacta
     9421 ttttagggta gaaaatggta tactatcttt aaagagatat aataaacttg agtttagaag
     9481 tagtacaaaa tttttttata ctaaaaatca cttaggcaat agagatgtga atgaggatga
     9541 tataagaaat atagtagtag attcaatagt aactcatatg gaagaggaga aagaaattgg
     9601 aacatttttg tctggtggaa tagattcatc tattataact actgtggctt cacaaattaa
     9661 tccaaatata aaatcttttt ctgcaggctt tagtgtcaaa ggatatagtg aattagaagt
     9721 agcaaagaaa acagctgata agttaggtat agaaaatatc cagattaata taacacaaga
     9781 tgaatatata aaatcattac cgaatgtaat ttattctttg gatgacccta tagcagaccc
     9841 ttcagaagta ggtctttatt tcttaattaa agaggcaggt aaacatgtta aagtagcatt
     9901 gtctggagaa ggagcagatg aattatttgg aggatataat atatataaag agtatagtac
     9961 tatgaaatca gttgtcaatt cacctactta tattaaaagt atattaggaa gagtttcaga
    10021 acttatgcca aacataaaag gaagaaatta tctttataga gctacaacac cattagaaaa
    10081 aagatatata ggaaatgcaa aagtttttga aaacagcgaa gtaaaaagat tcttttttaa
    10141 gtataaggaa aagaatatat atgaatacct gttgtctaat ttgtatagag atgcacaaaa
    10201 aaataattac gattatataa gtaaaatgca acatatagat gtgaatacat ggcttcaagg
    10261 cgatatactt caaaaaatta gcaaactttc tacagctgag caggttgaac ttagagtacc
    10321 atttttatat aaagatgtat ttgatgtagc aaaaaattta agaatggagc aaaaaataaa
    10381 taagaataat acaaaagttt tacttagaga ggcgtttaga gaaatagtcc cagaatatgt
    10441 tgttcaaagg aaaaaattag gatttccaac tccaataaga gtttggttaa aagattctct
    10501 aggggatatt gtaaaggaaa ctatttataa ttcaaatgta gatgaattta tagataaaaa
    10561 atatgcaata aaacttttag atacgcatct taaaggtcat agagataatt cgagaaaaat
    10621 atggactata tttacatttt gtctatggca tcaattattt attgagcata aaaatgtcga
    10681 gtattaatac ttttttcaat attaatcaga gggcaaaagc atgcaatgtt ttcaccctct
    10741 tgattattat ggaataggat aagtagaatt ttgattgatt agtttactat ctcaataaaa
    10801 ttattggcaa atattttgac aagattactt tcaacagcta aatttaagtt ttgtccatca
    10861 tcatatccag cggaaatcaa tcctagtaag tttccaaaca tatctaaaag agctcctcct
    10921 gaactaccac tagagattgg agctgtaaat tgtatcattt gaaccgtttc aaaatcacga
    10981 aaagctgaga taattccatc tgaaactgta ttaaaaagac ctaaagggct tccaattgca
    11041 actatttttt gacctctcac aggtttttta ctgactttaa caggtattgg tttgcatttt
    11101 ctatcaacct ttatcacagc taaatcataa tctgaatggt attttataat ttggtaagat
    11161 gtataaactt tatcatcatt ttcaaatcta actaaaaatg aatacccatc attgactaca
    11221 tgaagattcg taagtatata accttcatta tttattacta ctccagaacc actcttaaac
    11281 ggtttatttt cgttattgca cacaacaatc ataacaacag aagctgataa ctttgcaagg
    11341 tcttcataag aaagttctac ttcctcatct tgtgcaattt ttttagatgt tagatttgtg
    11401 ttattaagtg tatttttaga gttaggttta ttcgtagtag gagaagtctt attggtattt
    11461 tcactgtaat ttatatttgt aatatgcctt tcaaaaggta tttctaatat atttggagtt
    11521 ttcctagaaa gttcaaatag agtagaaaat tttgaacatt caggatataa atcaagaata
    11581 tctttatttg gatttatata taaaatatca cacccaagtt gtgataaaaa atataagaaa
    11641 aaaagttctt gccttttaat atttccaaag tatactatct ttggtgaaat agtagtcttc
    11701 accttgttat aaaataattt aggtagaaaa tagtccatcc aacacaaaag tttaactgca
    11761 aagtttttga tgatagagga tgtacaattt ggcatacttg tggaaaataa atctaaagta
    11821 tttttaaatg caatcttttt agtccaattt aaagtgtcat tttctatttt acatggaaag
    11881 tcgatattat ataaatccca actgttaaac ttagaactat cataaatatt aaaaatttta
    11941 agcatcttat ctgtttgttc tttgtcaaaa tttattttta gattgtttaa tctaatatag
    12001 ggaattgtag gattattaaa aaaataagta tctatatttt taactttttc tatgtattta
    12061 tcaattgaat catcaacacc tatatatctt gtaaagtgga cttcttt
//
LOCUS       DAELXX010000274         9754 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.guided.130, whole genome shotgun sequence.
ACCESSION   DAELXX010000274 DAELXX010000000
VERSION     DAELXX010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9754
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.guided.130"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            177..641
                     /locus_tag="KPH29_003773"
     CDS             177..641
                     /locus_tag="KPH29_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG1469289.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLAKLDVEVGFPVATYIDGKNEILQRIVPAQKIITAIDL
                     GPYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            939..1301
                     /locus_tag="KPH29_003774"
     CDS             939..1301
                     /locus_tag="KPH29_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG1469290.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
     gene            1896..2756
                     /locus_tag="KPH29_003775"
     CDS             1896..2756
                     /locus_tag="KPH29_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG1469291.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIIANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     CVEERDNRQN"
     gene            3014..5128
                     /locus_tag="KPH29_003776"
     CDS             3014..5128
                     /locus_tag="KPH29_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG1469292.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEHDVELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMAEKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHIVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REEYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYVGTLTQLISM"
     gene            complement(5390..7633)
                     /locus_tag="KPH29_003777"
     CDS             complement(5390..7633)
                     /locus_tag="KPH29_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG1469293.1"
                     /translation="MSNIIIKGLTQNNLKNISFKIPKNKIVVFTGVSGSGKSSIVFDT
                     IAAESQRQMNETYSSFIRNRLPKYRKPQVELIDNLTASVIVDQTPLGGNARSTVGTIS
                     DLYSSLRLLFSRIGEPYIGTTSYFSFNDPNGMCKNCSGIGRVKVINIESIINPENSWN
                     TGCINDSLFKPNSWYWKQYAESGLFDLDKPIKDYSFEEYNLLLYGSKNKDGKQENPKI
                     EGIFNKYNQTYLNRDISNMSKHTKEKSERLITDKECPVCHGKRLNKETLNCNILGYSI
                     ADMCNMELTDLYDLLLQIKDKRTETVIKTLTDGLKRMIDIGLPYLHLNRESSSLSGGE
                     AQRLKLVRYMGSSLTGMTYIFDEPSAGMHPRDVHRMNQLLKKLRDRGNTVLVVEHDKD
                     VISIADEVIDIGPSAGTQGGHVVFQGSYDKLLLANTLTSNAMNLSLPIKTDSRHPKGT
                     LPIRDACINNLKHIDVDLPVGIMTVVTGVAGSGKSTLISRVFAQKYEKDVIKIDQSPV
                     TATNRSMPATFLGFFDEIRKVFAKENNVEEGLFSFNSKGACPVCDGKGVLVTELVFMD
                     PIINTCEACKGTRYSEEVLSKLYKGKCIVDVLSMTVDESLEFFTEKKLCKILQTMKDV
                     GLPYMTLGQPLSTLSGGERQRIKLAKNLNKKGTIYILDEPTTGLHGSDIEKLMLIFEH
                     IVDKGNTVIIIEHNLDVLKQADWVIDIGPDGGKNGGQVVFEGTPLEMFERGCTITSDF
                     LRKSINI"
     gene            7965..8681
                     /locus_tag="KPH29_003778"
     CDS             7965..8681
                     /locus_tag="KPH29_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG1469294.1"
                     /translation="MILIVILSIFIIASINIIILGKENIRDIVIPLTLFIVSDFLFLC
                     GNIICRGGVMLLFIGLFAIPLMLIAFIWICVHMIKLYYKKDLNKRKMIGCIFSIFISI
                     GTIFIPSPSESFRFKLYEKDYSVVAKAILKVYDEKKLSSESVFYIQSDKNELEKIFSK
                     EVIKKMKKLNRNLDVVKISGNRNLVYFFFGAELQSIDGIVIFKDLKHVKPKLDFINRS
                     IQAPRLEYITNNVYYFKGEL"
     gene            8709..9647
                     /gene="blaCDD"
                     /locus_tag="KPH29_003779"
     CDS             8709..9647
                     /gene="blaCDD"
                     /locus_tag="KPH29_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBG1469295.1"
                     /translation="MKRKNNFIWIVILLVVVAMGVYYVGRKHNNTKQKHDKNHINSEL
                     NKSKVENNDKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETRRSPCS
                     TFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYYKKLID
                     KVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQTMAKI
                     FEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGMLEREGD
                     TYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
BASE COUNT         3735 a         1163 c         1534 g         3322 t
ORIGIN      
        1 gattataaga gcactgtgtg acaagaatga tgagattatt tttgctatac tttcgatata
       61 agaaatggaa ggaggttatg ttttatattt atatgaaata attagttaca cagtgaattt
      121 tcgtataatt tggtataaaa agattagaaa tgaaaataaa gtaatggagg aataaaatga
      181 ctagaatatc caatataaca atagctgaac aatctgagta ctgttttttg gcaatacgaa
      241 aaacaattga ctttatggta gagttttcag agttttcaaa acaaagtttt gaaaaaattt
      301 ctaaatatct ggaggagaga ggaattttat cttcaggagc accgattgta tgttttcata
      361 acatggattt agcaaaattg gatgttgaag tagggtttcc tgtagccact tatatagatg
      421 ggaaaaatga gattctacaa aggattgttc ctgcacagaa aataataact gcaattgatt
      481 taggacctta tgaattacaa gaccctactt tagaagactt attttcatgg gcaaatagca
      541 atggatataa acttcatggc gatatatatt accagtattt aaatgatatc aatcgtccag
      601 ctagtgaata tcttacgaaa atgatgttac ccattatata gaaagttaaa gattatcgtt
      661 tgttagttga taattagaag aacatttaat gctactgcat aaattttatc ctaaataata
      721 tcaaggtgat ttaatttatg tattctaatg taactgtaga taatcaattt ataactaact
      781 tagtgtaatt tagatttaat ttattattct aatgtaaccc caatatattg ttaaggtcta
      841 cttaagatgc tcaaaaggct atgcttaatt tattgttata taatttgtag agtggtataa
      901 tttagaagtg atactatata gatatatagg gggaaaatat gaaaatatat gatatattaa
      961 cacgttttta tattcaagat atagaaaaag ccataccttt ttatgagaat cttttaaaag
     1021 aaaaatgttc tttgcgtttt tcttataaag aagttgggtt ggaattagcg caaataggaa
     1081 atgttttact attatctggt tctgatgatg ctcttaaacc atttatagaa acaaaatcta
     1141 cttttatggt tgattctgtt gatgaatgga gaagttatct attggataat ggagcagttg
     1201 tagtaagaga taagaaaaaa gttcctacag gatataatat gacattgaga catccagatg
     1261 gtacaattat agaatatgtt caacatacaa agcaggatta gttaaaaata gattttacta
     1321 ctgagcaact aattagaatc tattcttaga cgtacaattt attatttttg gtattaatag
     1381 attataagtc aaattataat atttcaaaat agaatagaaa tttatttaca taaaagagag
     1441 tgtttaaaaa tgaacttttt agttcacgaa gcactccttt ttgtatgaaa tcaaatacat
     1501 caatatataa aacaaactga tgaagagctt aaattaaaat aaaaagattc ataacaggac
     1561 cccattaaac tttaagatag ggtaagtttg taatcatatt aaataattgt aaaaagctgt
     1621 ttttagtcgt ataatataaa tatttatcat ttaattaagg aatctaaaag gaatctaata
     1681 agaagaaaat aataaataac tttttagatt tactaaatga aaaattattg aaaattaaag
     1741 gattagagtc tttaatcaga aagtaaaaag taatatatta aatttatgaa ttgaaaactt
     1801 aaacaacaaa tttgtttata aaatagcaag attcattaaa tataactaag taataattga
     1861 tataatgttg catggataaa aggagggaag ctgcaatgtc ttacaaagta attaatgtca
     1921 taactgctat tgactatata gaggagcatt tatcagaaaa gctggattta gatataattg
     1981 ctaatgctgt tcattactca aaatatcacc tgcatagaac tttcacaact tctgttggtc
     2041 taacaatgca tgattacata aagcggagaa agttgacaga agcagcaaaa ttattggtat
     2101 tttcaaagaa accaattatt gaaattgcat tgatagctgg atatgaaagt caacaggcat
     2161 tcacttctat atttaaagct atgtataaaa aatctccaaa taaatatagg aaagaacaag
     2221 aattttatcc tttgcagcta agatttgttt tgaaaaaaga taacctattt tcaaagaatg
     2281 tactagagtt ggaaaaagaa attaaacttg caagtatgtc tgacatacca ttatggatga
     2341 atttagtgag attagtcata gatggatttc caaatttgca agaggaagaa tatatatatc
     2401 aattagaaca gtatatttta gaagaaagag ctttaatatt aaaacttaat aatgtcgcta
     2461 ttgcaaatat ggtgtttaat agagaaacta gaagtattga tttttttgga atacatccac
     2521 agtatcgtaa ttcagacatt gcacaagtgt ttttgaagaa agttattgaa gatttcttga
     2581 tagatacaga tatctcaatt actacctttc gagagggtga caaagctgat acaggacata
     2641 gagatatgat aaagaagttg gggtttgcag aagcagagtt gcttgtggaa tttggatatc
     2701 ctactcaaaa atttattctt ccttgtgtag aagaaagaga taatagacaa aattaattgt
     2761 agattaaatg caaagtttac cacatatttt ttgtttgaat tattttatat ataagagtat
     2821 ttctgctttt aatgtatttt gcttgtaagt gtacttatgg taataaatat agaaagattt
     2881 agttttttaa aaaaagaact ttaaagctaa aaagctaaca tttaagttaa atagcaagaa
     2941 tgattaaaaa ctccaaaaca caattaagta taatgattca gtgtaagtac ttaattaaca
     3001 aaggaggatt taaatgtctg aaaataaaca agtagtaaat tgcgatatta ttgcatccaa
     3061 ttctaaacat aatatgtgtg aacatgatgt agagttatta actttttttc cagatgagat
     3121 tgcacatcta atagagataa ataacaagct agacaatgct tttaagaaag ctgaaaatct
     3181 tgttgacaag cttgataagg attatatgga cgctaaaatg tatatggtaa aaaatcgtgg
     3241 tgaaatagac ccacatgaaa tgtttcaaaa tgaacaagga ctaaaacaaa tagataacta
     3301 tggggctttt atggtaaaag ttcgagataa gattaataaa atcaaagatt ctccatactt
     3361 tgctagaata gattttaggc tgaaagatat ggatgatgaa tctaagtatt atattggtag
     3421 atttgcattt gattatgaag atgagcttat aatacttgac tggcgttctc caattgccag
     3481 tatgttttat gattatgaaa tcggaaaagc tggatatgat gctccaatag gatgggtgga
     3541 tggagaaata acaagaaaac gacaatttaa aattaaaaat ggaaaattgg aatatgcact
     3601 agaaagttcc attaatatac aagatgatat tcttcaaaaa gaacttagtc acacttcaga
     3661 tgaaaaaatg aagtcaatca tttcaacaat acaaaaggaa caaaatcaaa ttattagaaa
     3721 tgataaagct gatacactga ttattcaagg ggtagctggt tctgggaaaa catctatagc
     3781 attacacaga attgcttttc ttctttatcg atttaaagat aaaatatcag caaataatgt
     3841 aattatccta tcacctaata aagtctttgg agactatatt tccaatgtcc ttcctgagct
     3901 tggagaagaa ccattatgtg aattaagttt tgaaaatata gcagaggtac aattagatag
     3961 agttattaat tttgaaagtg agaaagaccc attagaaata aatgatgcta aatggtctga
     4021 aagagtgcgt tttaaatcta cacttgattt tgtaaaactt attgatgatt atataaaaca
     4081 aatgccaaat aagattttta ttccaaaaga ctacactttt ggaagtttta cagctaaaag
     4141 tgactggata cagagtagat ttgaggcata taacagatat ccagttaaga agagattaga
     4201 aaaggtagct gaagacattc attataaatt tgaatcagat aatattatgg aagaagattt
     4261 accaaaggta aaaagtatac ttaaaagttt gaatggaatg ttgacaataa aaaatactct
     4321 tacactatat aaggatttct ttaaacaaat gaatatatca aacatgtttg tgatggcaga
     4381 aaagaaaaca cttgaatggt cagatgtata cccatttata tatattcatg ctgcatatga
     4441 aggtatacaa gaagataaga ttataaggca tattgttatt gatgaaatgc aggactatac
     4501 acctattcaa tatgctgtaa taaacttact ttttaagtgt aaaaagacta ttcttggaga
     4561 ttttggacaa ttggttaatc ctaatcatac tcatacatta gatgatatga aacagctata
     4621 taatgatggg gaactagtta tgttgaacaa gagttaccgt tctacttttg agattattaa
     4681 tttcgccaaa aagattcaag atatatcttc attagagcct attgaacgac atggtgaaga
     4741 accggcttta ttaaaatgta ataacgagca agatgaaata aataagataa aaatagaaat
     4801 tgaagaattt aaaaagagtg ataatgcgac tcttggtatt atattaaaga cagataatga
     4861 cgctaaagct gtatatgatg cgttaagaga agaatatagt gttcacttaa tatcttctga
     4921 aagttctagc tttacaaaag gagtatccat aacttctatt aaaatgtcaa aaggtttaga
     4981 gtttgatgaa gttattgtac cttcagtaaa taataaaaca tattatagtg attatgaccg
     5041 cagtttgtta tatattgctt gtacacgagc tatgcacaaa cttaagttaa catatgttgg
     5101 tacattaact caacttataa gcatgtagat tatatcttat atgcatttat tatcgtaaat
     5161 ataaattggg acaaagattc atcaataaat caacaaaaaa taatacttat aaatttataa
     5221 aaaatgaaag ctataaatta catttgaaat tttataaaat tttgtgattt atagctttta
     5281 taatattaat ttcttttaaa ataaagttaa actttaaatt tatgaaataa aacaaagtag
     5341 taatatatat gtaatagttc ttaatttttt aatattagta ttatattatc tatatattta
     5401 ttgattttct aaggaaatct gatgttatag tgcaacctct ttcaaacatt tctagtggtg
     5461 taccttcaaa tacgacctga ccaccgtttt tgccaccatc aggaccaata tcaattaccc
     5521 agtctgcttg tttcaataca tccaaattat gttcaattat aattacagta ttacctttat
     5581 caactatatg ctcaaaaatc aacataagct tttcaatatc agaaccatga agccctgtag
     5641 ttggctcatc taaaatatat atagtacctt ttttgttgag gtttttagct aacttaattc
     5701 gttgacgttc tcctccagat agtgtgctaa gtggttgacc taaggtcata taaggtaaac
     5761 caacatcttt cattgtttgt aagattttac atagcttctt ttctgtaaaa aattcaagag
     5821 attcatctac tgtcatagat agtacatcaa ctatacattt acctttatat aattttgaca
     5881 aaacttcttc actatatcta gtacctttgc aagcttcaca tgtattaata atagggtcca
     5941 tgaatacaag ctctgtaaca agtactcctt taccatcaca aacaggacaa gctccctttg
     6001 aattaaagct gaataaacct tcttcaacat tattttcttt agcaaatact tttcttatct
     6061 catcaaagaa accaagaaat gtagcaggca tagaacgatt tgtagctgta actgggcttt
     6121 ggtcgatttt aataacatct ttttcgtatt tttgtgcaaa aacacgagag attaaagtac
     6181 tcttgccaga acctgctact ccagtaacga cagtcataat tccaactggt aaatctacat
     6241 caatatgttt tagattattt atgcaagcat ctctaattgg aagggtacct tttggatgac
     6301 gagagtcagt ttttataggt aatgatagat tcattgcatt tgaagtaaga gtgtttgcta
     6361 aaagtaattt atcataagag ccttggaata caacatgtcc accctgagta ccagcacttg
     6421 ggccaatatc tataacttca tctgctatgg agataacatc tttatcatgc tctactacaa
     6481 ggacagtatt tcctctatct ctaagttttt ttaatagctg attcatacga tgtacatctc
     6541 gtggatgcat accagcacta ggttcatcga aaatataagt catacctgtg agactgctcc
     6601 ccatatagcg tacaagtttt aatctttgag cttctcctcc agatagagat gatgattctc
     6661 tatttagatg taggtaaggg agaccaatat caatcattct ttttaaacca tcagtaagag
     6721 tctttattac agtttcagtt cttttatctt ttatttgtaa caataaatca taaaggtcag
     6781 taagttccat attgcacata tctgcaatcg aatatcctaa aatattacag ttaagagttt
     6841 ctttatttaa tctttttcca tgacatacag gacattcctt atctgttata agtctttctg
     6901 atttttcttt tgtgtgtttg ctcatattgc taatatctcg atttagatat gtttgattgt
     6961 atttgttaaa aataccctct atttttggat tttcttgttt tccatcttta ttttttgaac
     7021 cataaagcaa taaattatat tcttcaaaag aataatcttt tataggttta tctaaatcaa
     7081 aaagtccaga ctctgcatac tgcttccagt accatgaatt aggtttaaat aaagagtcat
     7141 ttatacatcc tgtattccat gaattttcag gatttataat agattcaata ttgattactt
     7201 taactctacc aatcccagaa caatttttac acataccatt tgggtcatta aaagaaaaat
     7261 atgaagttgt tcctatgtag ggttctccaa ttctagaaaa taataatctt aatgatgaat
     7321 acaaatcact tatagtgcca actgtagaac gtgcattgcc accaagagga gtttggtcta
     7381 cgattacaga agcagttaaa ttatcaatca gctccacctg aggttttcta tatttaggta
     7441 gcctatttct gataaatgaa gaatatgttt cattcatctg cctttgtgat tctgctgcaa
     7501 ttgtatcaaa tacgatactg gattttccag aaccagaaac tccagtaaaa acaacaattt
     7561 tatttttagg aattttaaat gatatatttt ttaggttatt ttgtgtaagt ccttttatta
     7621 taatatttga catgaatgtt aaatcctttc tgagtacatt ttagtaaagc aattatatta
     7681 tgtaaccata ttataataaa attaaaatct actttcttga tatattctgc tatgttatag
     7741 aaggtatttc taaatttaac caatcccata caatctgaat aaatgatagt tgataataga
     7801 taaaaaagta tataatagat actcgtaatt tgataagtac aaagataatt atacaattaa
     7861 aacgacttga catttaaaac ttactattgc aatatttaaa tattacaata gtaagtttta
     7921 aatagagaat aaataattac ctcacaaact gaaaggagga cataatgata ctaatagtta
     7981 tattgagtat attcatcata gcctcaatca acataattat attaggtaaa gaaaatataa
     8041 gagatatagt tatcccttta actctattta ttgtaagtga ttttttattt ttgtgtggaa
     8101 atataatatg taggggagga gtaatgctac tatttatagg tttatttgca ataccattga
     8161 tgctaattgc atttatatgg atttgtgtac atatgattaa gctttactat aaaaaagatt
     8221 taaataaaag aaaaatgatt ggatgtatat ttagtatctt tataagtata ggtactatat
     8281 ttattcctag tccaagtgaa agttttagat ttaaacttta tgaaaaagat tatagtgtag
     8341 tagctaaagc tattttgaaa gtttatgatg aaaaaaagtt aagctctgaa tctgtctttt
     8401 atattcaatc tgataaaaat gaattggaga agatattttc taaagaagta ataaaaaaaa
     8461 tgaaaaaact aaacagaaat ttagatgtag ttaaaatttc tgggaataga aatttagtct
     8521 attttttctt tggagctgaa ttacaatcta ttgatggtat tgtgattttt aaagatttaa
     8581 aacatgttaa accaaagtta gattttataa atagaagtat tcaagctcct agattggagt
     8641 atattacaaa taatgtatat tattttaaag gtgaactata aacttatgta gaggagatgg
     8701 tataaagtat gaaaagaaaa aataatttta tttggatagt tattcttcta gttgttgtag
     8761 ctatgggagt atattatgta ggaagaaagc ataacaacac aaaacaaaaa catgataaga
     8821 atcatataaa ctctgaatta aataaatcca aagttgaaaa caatgataaa aacaaaaaag
     8881 aaaaaattaa tatggttgac tatagtgact gttttgaagg tattagtgga ggagctattt
     8941 tttataatac taaaaataaa gaatataata tatataacaa agaattaatt gaaacaagaa
     9001 ggtcaccatg ttctactttt aaaatagttt caactttaat tggtttagaa aaaggggtaa
     9061 taaactcaaa agaatcagtt atgggatatg atggtacaga gtatccaaat aaaaattgga
     9121 ataaaaatct tagtttagaa gaggcattta aggagtcttg tgtttggtac tacaaaaaat
     9181 tgatagataa agttgatgct aaatctgtcc aaaacatttt agatgattta aagtatggga
     9241 attgcgatat tagcgaatgg gaaggagatt taaaaaatgg aaagggacat ttaaatggat
     9301 tttggttaga atcttcatta caaatatctc caaaagaaca agtgcaaaca atggcaaaaa
     9361 tatttgaggg agatacaaac tttaaaaaag agcatattaa tattttaaga gatattatga
     9421 aaatagatgt aaatgataaa aacataaatg tatatggaaa aactggaaca ggatttgatg
     9481 aaaaaaataa acgtgtggat gcatggtttg ttggtatgct agagcgtgaa ggtgacacct
     9541 attattttgc tattaaatca gatgattcca ataaagaaat tacagggcca aaggtaaagg
     9601 aaattgcaat taatataatt aagaaatatt atagtgtaag ggaataaact taattttatt
     9661 tgtaaatatt atttacctat agtctgtaat atttattaaa taattttact aaattgagtc
     9721 tatatagagc aaaactcctt tagtacataa aata
//
LOCUS       DAELXX010000275         2848 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.guided.217, whole genome shotgun sequence.
ACCESSION   DAELXX010000275 DAELXX010000000
VERSION     DAELXX010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.guided.217"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            53..733
                     /locus_tag="KPH29_003780"
     CDS             53..733
                     /locus_tag="KPH29_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG1469296.1"
                     /translation="MQTKILIIDGDKDNCQKLKGFLEEKEISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILTDGDGWTLCKKIRNVTTCPIVYMTYINEEQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            1115..1813
                     /gene="vanR"
                     /locus_tag="KPH29_003781"
     CDS             1115..1813
                     /gene="vanR"
                     /locus_tag="KPH29_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HBG1469297.1"
                     /translation="MSGKILVVDDERDIADLIEVYLTNENYIVYKFYSGLDALNCINL
                     NEFDLAILDVMLPGVNGFSLCQKIREKYTYPIIMLTAKDEETDKITGLTLGADDYVTK
                     PFRPLELVARVKAQIRRYKKYSGSLQSGEELSHIFSFLGLELNTQTYECHLNGKALSL
                     TPTEFSILHVLLKNKGSVVNSENLFHAIWKDEYYNKSNNTITVHIRHLREKLGDTVDE
                     AKYIKTIWGVGYKI"
     gene            1849..>2848
                     /locus_tag="KPH29_003782"
     CDS             1849..>2848
                     /locus_tag="KPH29_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG1469298.1"
                     /translation="MKKINNFTYRKLRDKYKFIKREKNTEAYLKYKLYIMLAIYTGIV
                     LVLMFMADYTFSNFSNPLVKWLKLRRDVLFFLVFIIGYITIFNFYWKKPWNYLREIIN
                     ATHTIYQQNDELVELSKPLKEIEQQMNQIKMSVLLSQQAVKEAENKKNNLVMYLAHDI
                     RTPLTSVIGYLSLLKEIPNMDNSQKSKYTEVALDKAQYLEKLINELFEITRYNSNQIK
                     INFEKVDLYYMLLQLKDEFYPLLSEKGNRAILKANESLTIKADSEKLARVFNNILKNA
                     IAYSYPNTEIIISAVHKDSKVDIAFRNYGQTIPEEQLSAIFEKFNRLDEARKSDMGG"
BASE COUNT         1161 a          302 c          445 g          940 t
ORIGIN      
        1 taataatagc ttatttaata taaaagaata atgatatttg ggagtgatta taatgcaaac
       61 taagatattg ataatagatg gagacaagga taattgccag aagctaaaag gatttctgga
      121 ggaaaaagaa ataagtatag atttagcata taattgtgaa gaagctattg gaaaaatatt
      181 tagcaataaa tatgatttaa tatttttaga aataattcta actgatggtg atggatggac
      241 tctttgtaaa aaaattagaa atgtaactac ttgtccaatt gtttatatga cttatataaa
      301 tgaagaacaa agcattttaa atgctttaaa ttctggcgga gatgattact taataaagcc
      361 tctaaattta gaaatattat atgcaaaagt aaaagctatt ttaagaagaa tgaactcata
      421 tgtaaataat aatgaaaata atacaaaatt agaggaatat aatagaacta gtggagtaat
      481 aaagttagaa gacaaaataa taaaattgac tccaacagaa aataagctgt taaacttttt
      541 tatagagaat cctgaaaaga ctctgactac taaagagata tatgaacatg tatggatgaa
      601 tgagtactta gaggataact atagtgtggt agttgcagtg aatggattaa gaaagaaaat
      661 agagaaagat tataaaaatc ctcagaaaat tataacaata agaggagcag gatattattt
      721 taataaagaa taataaatat attaatctca ttaataattg ttataaatgt gaaatgctaa
      781 cgatatctaa actaaattat tataaaatag ctgactgtaa tttaattgat aaagcctatt
      841 cttctagact ttgccagtta aattacagtc agttttatta tatgaaaaaa tgcgtaacag
      901 aactattatt ttatagatta tatttgaaaa gatagataaa ttgtatatct aaatctatat
      961 atattcattt ttatctttaa tatatgttaa ataataaaat agttacataa taagattaag
     1021 aatttcttaa taaattctaa tgtaaaaaat catataaaaa aattaaattt agtataatat
     1081 atataaatat atatagaaag gatgtttaaa gtctatgagt ggaaaaattt tagttgtaga
     1141 tgacgaacgt gatatagctg atttaataga agtctacctt acaaatgaaa actatattgt
     1201 ttataaattt tattctggtc ttgatgcact aaactgcatt aatttaaatg aatttgacct
     1261 tgcaatttta gatgttatgt tgccaggagt aaatggattt tctttatgcc aaaaaatccg
     1321 agaaaaatat acttatccaa tcattatgtt aacagctaag gatgaagaaa cagacaagat
     1381 tacaggctta actttaggtg cagatgatta tgttactaaa cctttccgac ctttggaact
     1441 tgttgctaga gtgaaggcac agattagaag atataagaaa tacagtggaa gtttacaatc
     1501 tggtgaagaa ctttcacata ttttttcttt tttaggtctg gaattgaata cccaaacata
     1561 tgaatgccat ttaaatggta aagctctttc acttactcca acagagtttt ctattctaca
     1621 tgtattattg aaaaataaag gctctgtggt aaattcagaa aatctttttc atgctatctg
     1681 gaaagatgaa tattacaata aaagtaacaa tactataaca gttcatattc gccatcttcg
     1741 tgaaaaatta ggtgatactg tagatgaagc aaaatatata aaaacaattt ggggtgttgg
     1801 ttacaagatt tgacacctat ttttcttgtc aagaagggat gtagtattat gaaaaagatt
     1861 aataatttta cttataggaa actaagagat aaatataaat ttataaaaag agaaaaaaat
     1921 acagaggcgt atttaaagta taaattatat ataatgttag ctatctatac aggtatagta
     1981 ttggttttaa tgtttatggc tgattatacg ttttcaaatt tttcaaatcc tcttgttaaa
     2041 tggcttaagt tgagaagaga tgtattattt tttttggttt ttataattgg atatatcact
     2101 attttcaatt tttattggaa aaaaccatgg aattatttaa gagaaatcat caatgcaaca
     2161 catactattt atcaacaaaa tgatgaattg gttgaactat ctaagccatt aaaggaaata
     2221 gagcaacaaa tgaaccaaat taagatgtct gttttgttaa gtcaacaagc agtgaaagag
     2281 gctgaaaata aaaagaataa ccttgtgatg tatctagcac atgatataag gacaccactt
     2341 acttcagtta ttgggtattt gagcttatta aaagaaatac ctaatatgga taattctcaa
     2401 aaatctaagt atactgaagt tgcacttgac aaagcacaat atttagaaaa acttatcaat
     2461 gaactttttg aaataacacg atataattct aatcaaatca aaattaattt cgaaaaagta
     2521 gacctatatt atatgttact tcaattaaag gatgaatttt acccattgct gtctgaaaaa
     2581 ggaaatagag ccattctaaa agcaaatgaa agtttaacaa taaaagcaga ttctgaaaaa
     2641 cttgcaagag tgtttaataa tatcttgaaa aatgctattg cttacagcta tcctaataca
     2701 gaaattatta tctcagcggt tcataaagac agcaaggtag atattgcttt tagaaattat
     2761 ggacagacaa ttccagaaga acaactttct gctatatttg aaaagttcaa tcgtttagat
     2821 gaagctagaa aatctgatat gggtggtg
//
LOCUS       DAELXX010000276         1829 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019870
            SAMN15907332-rid12312393.guided.240, whole genome shotgun sequence.
ACCESSION   DAELXX010000276 DAELXX010000000
VERSION     DAELXX010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907332
            Sequence Read Archive: SRR12520603
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050455.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:06:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,784
            CDSs (total)                      :: 3,700
            Genes (coding)                    :: 3,651
            CDSs (with protein)               :: 3,651
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907332-rid12312393.guided.240"
                     /isolate="M2016019870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-05"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..164)
                     /locus_tag="KPH29_003783"
     CDS             complement(<1..164)
                     /locus_tag="KPH29_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBG1469299.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFE"
     gene            970..1602
                     /locus_tag="KPH29_003784"
     CDS             970..1602
                     /locus_tag="KPH29_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBG1469300.1"
                     /translation="MTIPNSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVSPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
BASE COUNT          726 a          225 c          202 g          676 t
ORIGIN      
        1 ttctcaaaaa aatcaactcg ccactctata atatccaaac atgcatcttt taactcttta
       61 gcctctttta taatatcttt tttatttttc ccaataattg gtacacaaat tttaggtcta
      121 ccttctccaa tagtaatatt ttttacttga acttttcttt tcatattctc ctccataatc
      181 agaaattcta taggacttta aattctattt tctgcataat ttaattatac attaaaaata
      241 ctatacatta taaaaatact aatttaaaaa taatttttat tctaattctt aaaacaatcc
      301 cacatatttt agaaaaggta ctctataata tacaaacact tcttttttac ctaaatttat
      361 tttaacattc tataattttc acaatattct ttaaatttta ctcatataaa caatattatt
      421 ctggatgcta atagaattaa tctataaatc atagtataat tatgcagaaa taactcgaat
      481 ttcactactt ataaagagat tgaatattaa tgaatctaaa tcatttgcat tacttcagag
      541 ttttgactaa agttgaacac catacaaaag tagctactca attgtttata ccccaaccaa
      601 gcttaagtaa tgttatatag attcttgaaa aagaatttgg tatcttttta tttgaaaaac
      661 aaggtagaaa tgtttccctc actaaatacg gaagtttttt tctaacctat gttgataaag
      721 cattaggtta attagaaaaa gtttacatat aaaatatatt aaattgttgc aagttttatt
      781 gttttgatat acaatcaact aaacatataa tctttataaa atactataca gttaaaagtc
      841 tgttttaaaa aaaaatgtat aaattataaa tcaaaatata gatactatca aggagtgatt
      901 ctgtgaagag atgtattgta gtagtggaat agtcaaaaac ataatatata ttaaaggaga
      961 gatttaaata tgactattcc aaattcaaat aaggtatatc caagaagtaa tgattatcaa
     1021 actgtatatc ttaaaaatgt aataacaagg gaaaatatag aagttggaga ttacacaata
     1081 tataatgact tttataatga cccaagagat tttgagaaaa acaatgtatt atatcattac
     1141 cctataaaca atgataaatt aataattggt aagttttgct caatagcatg taatgcaaaa
     1201 tttctaatga ctagtggaaa tcactcaatg aaatcaaaat ccacatatac ttttcccatc
     1261 ttttatgaag aatggaaact cgatatcaac catataacgg acgcttggga caataagggt
     1321 aacattataa ttggaaatga tgtgtggata ggttatgatt ctataattat gtcaggtgta
     1381 aatattggtg atggtgcaat tattggaaca agagcagtag ttactaatga cgtctcaccg
     1441 tattctattg ttggaggagt acctgcaaaa attataaaaa agagatttaa taatgataca
     1501 atttcaaaat tattagaaat taaatggtgg gattggccat ataaaaaaat tcaatcaaac
     1561 attaagcata ttcaatcagg aaatattgat aaattagttt aatattttat aatttattta
     1621 tattaaactt tactttaata atccttttat aagagctttt tcaaaatttg tatattattt
     1681 taatatatat aaatttaata caacattaaa taaaataaaa agtaaagtta cttaataaaa
     1741 agttacttta ctttttatta tttaaattcc tgtttttatt ataataggta aaattctatt
     1801 aaactagttc acattttcat tattattga
//
